Review for 10.1186/s12876-016-0477-5 (2016)

Reviewer ’s report  
 
Title: Development of a novel mouse model of hepatocellular carcinoma with nonalcoholic 
steatohepatitis using a high -fat, choline -deficient diet and intraperitoneal injection of 
diethylnitrosamine  
 
 
Version: 1 Date: 11 Dec 2015  
 
 
Reviewer: Atsushi  Nakajima  
 
 
Reviewer's report:  
 
The manuscript is carefully revised and worth publication. However, there are some simple 
problem. 
 
1. The authors should mention how they obtained HFCD. In page 7 line 116, is references 
25 right?  
  
2. The authors used Osirix softwear to measure tumors. They should mention how they 
define the nodules as tumors. For example, about the size of nodules or about the views 
of CT image.  
 
3. In line 347, there is not fig 8.  
 
Are the methods appropriate and well described?  
If not, please specify what is required in your comments to the authors.  
 
Yes 
 
Does the work include the necessary controls?  
If not, please specify which controls are required in your comments to the authors.  
 
Yes 
 
Are the conclusions drawn adequately supported  by the data shown?  
If not, please explain in your comments to the authors.  
 
Yes 
 
Are you able to assess any statistics in the manuscript or would you recommend an  
additional statistical review?  
If an additional statistical review is recommended, please spec ify what aspects require further  
assessment in your comments to the editors.  
 
I am able to assess the statistics  
 
Quality of written English  
Please indicate the quality of language in the manuscript:  
 
Acceptable  
 
Declaration of competing interests  
Please complete  a declaration of competing interests, considering the following questions:  
 
1. Have you in the past five years received reimbursements, fees, funding, or salary from 
an organisation that may in any way gain or lose financially from the publication of this  
manuscript, either now or in the future?  
 
2. Do you hold any stocks or shares in an organisation that may in any way gain or lose  
financially from the publication of this manuscript, either now or in the future?  
 
3. Do you hold or are you currently applying for any patents relating to the content of the  
manuscript?  
 
4. Have you received reimbursements, fees, funding, or salary from an organization that 
holds  or has applied for patents relating to the content of the manuscript?  
 
5. Do you have any other financial competing interests?  
 
6. Do you have any non -financial competing interests in relation to this paper?  
 
If you can answer no to all of the above, write 'I declare that I have no competing interests' 
below.  If you r reply is yes to any, please give details below.  
 
I declare that I have no competing  interests  
 
I agree to the open peer review policy of the journal. I understand that my name will be included  
on my report to the authors and, if the manuscript is accepted for publication, my n amed report  
including any attachments I upload will be posted on the website along with the authors'  
responses. I agree for my report to be made available under an Open Access Creative Commons  
CC-BY license ( http://creativecommons.org/licenses/by/4.0/ ). I understand that any comments  
which I do not wish to be included in my named report can be i ncluded as confidential comments  
to the editors, which will not be published.  
 
I agree to the open peer review policy of the journal