JerMa88/BMC_Gastroenterology_Peer_Reviews huggingface dataset:
Review for 10.1186/s12876-024-03421-6 (2024)

Title: Adenocarcinoma of the esophagogastric junction: characteristics of female patients
and young adult patients based on a 12-year retrospective and prospective multicenter
clinicoepidemiological cohort study in Japan
Version: 6 Date: 16 Sep 2024
Reviewer's report:
This manuscript has reported clinicoepidemiological characteristics of female and young adult
patients with adenocarcinoma of the esophagogastric junction (AEGJ) and the 12-year trends
in the Kurashiki area for young adult patients with AEGJ.  The results are very interesting and
helpful for future clinical practice.
This is the first time I have reviewed this paper, but I believe that it is a revised version that has
already been peer reviewed by the other experts.  I have a feeling that the paper has responded
appropriately to many of the reviewers’ concerns, and I think it can be accepted for publication.
Review for 10.1186/s12876-024-03439-w (2024)

Title: A method of “Noninjecting Resection using Bipolar Soft coagulation mode; NIRBS” for
superficial non-ampullary duodenal epithelial tumor: A pilot study
Version: 5 Date: 19 Aug 2024
Reviewer's report:
In this paper, Tokuhara M et al. reported the efficacy and safety of a novel endoscopic
resection method, "Noninjecting Resection using Bipolar Soft Coagulation Mode (NIRBS),"
for superficial non-ampullary duodenal epithelial tumors (SNADETs). Given the technical
challenges and high risk of complications associated with endoscopic treatment of duodenal
lesions, the development of a new technique is highly significant. Moreover, despite the
challenges posed by biopsy scars, en bloc resection was successfully performed in two patients
with such scars, although endoscopic resection (ER) can be difficult in these cases. No adverse
events (AEs) such as perforation or bleeding occurred in this study. Therefore, NIRBS appears
to be highly suitable for ER of SNADETs. Based on these findings, I recommend accepting this
manuscript for publication; however, I suggest that the authors consider one revision.
1) While this study strongly supports the efficacy and safety of NIRBS, I recommend adding
a more detailed comparison between NIRBS and other existing resection techniques, such as
Endoscopic Mucosal Resection (EMR) and Endoscopic Submucosal Dissection (ESD), in the
discussion section. For example, you could compare the shorter procedure time, lower risk of
complications, or the simplicity of the NIRBS technique relative to these other methods. This
comparison would clarify the relative advantages and limitations of NIRBS, making it easier for
readers to determine how best to apply NIRBS in clinical practice.
Review for 10.1186/s12876-024-03439-w (2024)

Title: A method of “Noninjecting Resection using Bipolar Soft coagulation mode; NIRBS” for
superficial non-ampullary duodenal epithelial tumor: A pilot study
Version: 5 Date: 21 Aug 2024
Reviewer's report:
Thank you for asking me to peer review this paper.
This paper is a retrospective pilot study reviewing a novel technique for removal of mucosal
lesions in the duodenum.
The premise for the normal technique is that existing mucosal resection techniques have
limitations with unacceptable adverse events. The opinion of the authors is that endoscopic
mucosal resection (cold and with cautery) and ESD are consistent with poor outcomes with a
high risk of adverse events.
The nature of the lesion was not confirmed with biopsy prior to endoscopic resection with the
novel technique. The technique essentially involved snare resection with non-injection using
soft coagulation.
The resected lesions were located in the bulbar duodenum 23%, proximal D2 54% and distal
D2 23%. 13 specimens demonstrated en bloc resection.
This is a small study including only 13 patients. One could question whether it is a true pilot
study if it is retrospective in nature.
The study is insufficiently powered to suggest that this technique is of any great benefit compared
to more widely accepted methods of endoscopic resection in the duodenum.
I would disagree with comments made about preinjection in the duodenum ("poor mucosal
lifting") although certainly this can be hindered in the first part of the duodenum. There are
no issues with mucosal lifting in the second part of the duodenum. The likelihood of a patient
having an adenoma in the bulbar duodenum amongst Brunner's glands is very low.
I would also disagree with the comments made about cold snare polypectomy, which is widely
utilised for small duodenal adenomas after preinjection. The risk of bleeding after cold snare
polypectomy of the duodenum after preinjection is negligible, particularly if prophylactic clip
placement is used.
I strongly agree with the comments made by the authors about performing ESD in the duodenum.
The authors do not mention the size of the snare. The size of the snare would be very important
particularly when suction is involved to engage tissue for resection. The size of the snare should
be included in the methods in case this technique is attempted with a different brand of snare
by readers.
Soft coagulation is not an innocuous current. It does have a significant depth of penetration,
although the relatively low power rating is noted. Despite the jiggling method, I would be
concerned about the potential risk of superficial or deep muscle injury  for lesions up to 20 mm
in diameter.
I disagree that conventional EMR is unsuitable for the duodenum "due to a perceived risk of
perforation caused by insufficient mucosal lifting. "This is inconsistent with published data.
I hope that my review is helpful to the authors.
Review for 10.1186/s12876-024-03439-w (2024)

Title: A method of “Noninjecting Resection using Bipolar Soft coagulation mode; NIRBS” for
superficial non-ampullary duodenal epithelial tumor: A pilot study
Version: 6 Date: 31 Aug 2024
Reviewer's report:
As highlighted in my original comments, I disagree with the comment in the introduction
that present techniques (except for ESD) are high risk for removal of duodenal adenomas.
Sessile polyp preinjection and resection with cold snare or electrocautery snare really very safe,
particularly in the small mucosal defects that can be closed with clips.
This normal technique described in this pilot study can only really be described as an alternative
to existing techniques. Whether it did indeed has an improved safety profile could only be
demonstrated in a larger study.
Suggest review correct dimensions of the snare as edit suggests that this snare is 6 cm in length !
Review for 10.1186/s12876-024-03440-3 (2024)

Title: Magnetic Navigation-Assisted Colonoscopic Enteral Tube Placement in Swine (with
Video): A Preliminary Study
Version: 0 Date: 21 Jun 2024
Reviewer's report:
This study employs an intriguing and novel technique using magnetic navigation to assist in
inserting a tube into the colon of swine during colonoscopy, demonstrating both feasibility and
safety. However, several issues require modification.
The authors highlight some shortcomings of another technique, colonic TET, including a longer
operation time. However, according to the first reference cited in this manuscript, the time for
the colonic TET procedure was 14.8 ± 5.8 minutes, while the median operation time for the
insertion of the MNT-assisted colonic TET tube was 30 (26–47) minutes. These data contradict
the opinion presented in the current article.
Although the authors suggest that the tube could be used for repeated treatments, this study did
not evaluate the retention time of the tube. If the tube needs to be inserted repeatedly, requiring
repeated preoperative bowel preparation, the value of this technique would be limited.
The authors mentioned that with MNT assistance, the tube's diameter could not be limited by
the colonoscopy channels. However, they may have overlooked patient compliance in clinical
practice, which is closely related to the tube diameter. A larger diameter often results in lower
patient compliance. Therefore, relevant studies should be discussed.
In addition to discussing colonic TET, the authors should also address the transanal ileus
catheter, providing a more in-depth and comprehensive discussion of these techniques.
The language of this manuscript should be polished by a native speaker for clarity and fluency.
Review for 10.1186/s12876-024-03440-3 (2024)

Title: Magnetic Navigation-Assisted Colonoscopic Enteral Tube Placement in Swine (with
Video): A Preliminary Study
Version: 0 Date: 02 Jul 2024
Reviewer's report:
# am not sure on what this study is trying to tell us.
The tube did not reach its endpoint - ie the proximal colon, which was the entire purpose.
The comparison  of previously used clips VS the magnetic device  to secure the tube in place is
not clear - is the tube supposed to stay long-term for FMT? And is the patient supposed to stay
permanently attached to the magnet until completion of treatment?
The mentioning of magnetic placement of NJ tubes and how they stay in place is also not
particularly relevant
Review for 10.1186/s12876-024-03420-7 (2024)

Title: Multicenter analysis of the efficacy of early cholecystectomy and preoperative
cholecystostomy for severe acute cholecystitis: A retrospective study of data from the multi-
institutional database of the Hiroshima Surgical Study Group of Clinical Oncology
Version: 5 Date: 09 Aug 2024
Reviewer:  Ana Gonzalez-Castillo
Reviewer's report:
Dear authors, congratulations for all the work and the valuable results achieved. This is the way
we have to go with Acute Cholecystitis. But, let me make few suggestions:
- Reading methods, I cannot be sure if the quantitative variables follow a normal distribution,
could you please specify? As I can understand the results are in median and IQR, so they are
not following a normal distribution. Describe also the test you use to check the distribution of
the quantitative variables.
- In methods as-well, I am not able to find the indications to perform the PC. Could you
specify? Also in this topic, is important to know if they where transperitoneal, transhepatic, and
if you studied the common biliary duct with a cholangiography through the CP. It is valuable
information for the community.
- In conclusions you should specify that not only PC before cholecystectomy prevent from
bleeding and hospital stay length, the most important one, is the recurrence. You should mention.
- About sintaxis, PC after cholecystectomy could confuse the reader, consider to use PC before
cholecystectomy and to specify clearly that it is CP + Surgery in same admission.
- In Figure one: I am not able to understand it. You could make it more illustrative with numbers
of percentage in each column of may be, you could change it with a Patient Accounting figure,
that it is missing.
Thank you very much for your effort and to study about acute cholecystitis, this results will
improve patients outcomes.
Review for 10.1186/s12876-024-03420-7 (2024)

Title: Multicenter analysis of the efficacy of early cholecystectomy and preoperative
cholecystostomy for severe acute cholecystitis: A retrospective study of data from the multi-
institutional database of the Hiroshima Surgical Study Group of Clinical Oncology
Version: 5 Date: 09 Aug 2024
Reviewer's report:
Accept for publication. Furthermore, the manuscript would benefit from a more nuanced
exploration of potential avenues for future research. Integrating perspectives from recent works
such as [PMID: 33915091], [PMID: 33915091] and [PMID: 38533026]  could offer valuable
insights.
Review for 10.1186/s12876-024-03420-7 (2024)

Title: Multicenter analysis of the efficacy of early cholecystectomy and preoperative
cholecystostomy for severe acute cholecystitis: A retrospective study of data from the multi-
institutional database of the Hiroshima Surgical Study Group of Clinical Oncology
Version: 5 Date: 19 Aug 2024
Reviewer:  Samuele Vaccari
Reviewer's report:
Well-written and well-argued paper. The authors wanted to investigate the impact of pre-
operative percutaneous drainage in cases of grade III acute cholecystitis according to Tokyo18
Guidelines.
Despite the authors' limitations listed in the paper, we can get good information about this topic.
However, some points should be explored in depth for a better understanding of the study:
1. Were the patients undergoing percutaneous drainage operated on during the same
hospitalization? Specify the time between drainage placement and cholecystectomy.
2. Did the teams of surgeons use a common scheme, or did they decide based on their own
experience?
3. Please explain better when you performed the percutaneous drainage (characters of patients,
of disease, etcetera)
4. Perhaps it would be appropriate to specify the six mortality cases, highlighting the differences
or the things in common with the cases.
After these insights, we can consider the possibility of publishing the paper.
Review for 10.1186/s12876-024-03420-7 (2024)

Title: Multicenter analysis of the efficacy of early cholecystectomy and preoperative
cholecystostomy for severe acute cholecystitis: A retrospective study of data from the multi-
institutional database of the Hiroshima Surgical Study Group of Clinical Oncology
Version: 6 Date: 08 Sep 2024
Reviewer's report:
The manuscript presents a comprehensive and well-structured retrospective study evaluating
the timing and effectiveness of early cholecystectomy and preoperative percutaneous
cholecystostomy (PC) for patients with severe acute cholecystitis. The study provides valuable
insights into the optimal treatment strategies for high-risk patients, and the data derived from
a multi-institutional database strengthens the reliability of the findings. Overall, the manuscript
is well-written, and the research methodology is sound. The statistical analyses are appropriate,
and the conclusions are well-supported by the data.I recommend the manuscript for publication
Review for 10.1186/s12876-024-03420-7 (2024)

Title: Multicenter analysis of the efficacy of early cholecystectomy and preoperative
cholecystostomy for severe acute cholecystitis: A retrospective study of data from the multi-
institutional database of the Hiroshima Surgical Study Group of Clinical Oncology
Version: 6 Date: 13 Sep 2024
Reviewer:  Ana Gonzalez-Castillo
Reviewer's report:
Congratulations again on your project!
I wanted to take a moment to acknowledge the effort, patience on my comments  and interest
you've put on this. Well done!
Review for 10.1186/s12876-024-03338-0 (2024)

Title: Surgical treatment of chronic pancreatitis with an inflammatory pancreatic head mass: a
retrospective study
Version: 1 Date: 29 Apr 2024
Reviewer's report:
Comments and questions:
The manuscript deals with a relevant question, namely the importance of early surgery for
patients suffering from chronic pancreatitis. The study is well-done.
1. Why the authors prefered the Beger procedure, instead of the Berne modification? The latter
is shorter, easier to perform, and is associated with the same outcomes.
2. Did recurrent cholestasis occur after the DPPHR-s?
3. How the authors select the patients for Beger or Frey procedure?
4. What was the surgical solution of the cholestasis during DPPHR?
5. What was the reason for the relaparotomies?
6. The ratio of the CR-POPF was relatively high in this series. What can be the reason of it?
At chronic pancreatitis, the fibrotic parenchyma makes the anastomosis safer, than in case of
soft pancreas.
7. Do the authors have late follow-up data of the early part of the series, for example from the
period of 2014-2018?
Review for 10.1186/s12876-024-03338-0 (2024)

Title: Surgical treatment of chronic pancreatitis with an inflammatory pancreatic head mass: a
retrospective study
Version: 1 Date: 21 May 2024
Reviewer's report:
The authors present a retrospective short-term analysis of 61 patients undergoing surgery
for advanced chronic pancreatitis, comparing postoperative short-term results of 38 patients
following PD with 28 patients following DPPHR.
They concluded that both surgical techniques are safe treatment options. The parenchyma-
sparing pancreatic head resection, DPPHR was superior in terms of shorter operation time,
however most intra- and postoperative parameters were observed equally frequent. This
retrospective analysis is based on a very small patient group. The message for surgical decision-
making to choose the best procedure could be improved by presenting more detailed analytic
data.
1a. What was the frequency of reintervention in both groups of patients (Radiologic management
of arterial bleeding, interventional abscess drainage, biliary stenting, endoscopic intervention)
hidden in Table 3:  CD III+ (32 % vs. 35 % respectively PDE vs. DPPHR)?
1b. How many patients were discharged with drainage of a biliary fistula in situ in both groups?
1c. The frequency of re-laparotomy is unusually high with 18 % PDE and 23 % DPPHR. More
convincing are data about indication of relaparotomy in the 7 and 5 patients respectively.
1d. What was the frequency of 90-day rehospitalisation in both groups?
2. How many patients suffered cancer in the pancreatic head with inflammatory head tumor
in both groups of patients?
3. To include Frey procedure in the DPPHR is incorrect, since Frey procedure is a modification
of Partington-Rochelle with coring out of some preductal pancreatic head tissue.
4. The Cochrane meta-analysis of randomized trials (Cochrane database systematic review 2016)
is overestimated. Its evidence is low because of inclusion of trials with several modifications
of DPPHR (Frey procedure, Izbicki-technique) and two PDs: Whipple procedure and PPPD in
the group of PD.
5. The authors did not consider the long-term outcome after PD and DPPHR in terms
of metabolic complications, e. g. new onset of diabetes mellitus, new onset of exocrine
insufficiency with the need of life-long medical enzyme supplementation.
Long-term observation after PD and DPPHR for chronic pancreatitis revealed a significant
shorter long-term survival after 15 years following PD compared to DPPHR (Ann Surg 2013;
258 (5): 815-).
In the present form of data analysis, the message of the manuscript is limited.
Review for 10.1186/s12876-024-03338-0 (2024)

Title: Surgical treatment of chronic pancreatitis with an inflammatory pancreatic head mass: a
retrospective study
Version: 2 Date: 14 Jun 2024
Reviewer's report:
1.The authors present in their point-to-point answer and in the revised manuscript important
details of the early postoperative complications comparing PD and DPPHR for chronic
pancreatitis with inflammatory mass in the pancreatic head. The in-hospital mortality was 5 %
vs. 0 % following PD resp. DPPHR. The author should include these data in the abstract.
2. The message in ‘Conclusions’ (Abstract and Discussion) is misleading regarding the
advantages of the parenchyma-sparing resection compared to PD in terms of high risk for
complications after PD with a mortality of 5 %, maintenance of the duodenum and upper loop
of jejunum and lower risk of metabolic dysfunctions displayed postoperatively after DPPHR,
when compared to PD.
Review for 10.1186/s12876-024-03422-5 (2024)

Title: Integration of bile proteomics and metabolomics analyses reveals novel insights into
different types of gallstones in a high-altitude area
Version: 3 Date: 08 Aug 2024
Reviewer:  Zhanlong Mei
Reviewer's report:
This article contains many obvious errors, and the methodological descriptions are also very
inadequate. The presentation of data quality control is insufficient and requires careful review
and the provision of comprehensive quality control data.
Review for 10.1186/s12876-024-03422-5 (2024)

Title: Integration of bile proteomics and metabolomics analyses reveals novel insights into
different types of gallstones in a high-altitude area
Version: 3 Date: 15 Aug 2024
Reviewer's report:
I have read the manuscript carefully.
The study used proteomics and metabolomics to genomically analyse the bile of patients with
two types of gallstones, which has some clinical significance, however, I have the following
specific comments on this manuscript#
1#In the overall experimental design it was stated that this study included 20 patients with CGD
and 30 patients with PGD, however the bile collection process was described as collecting bile
specimens from 40 patients in both groups after surgery. What about the other 10 patients?
2#Metabolomics took 20 specimens from each group of patients and 9 specimens from each
group for proteomics, however, this was not stated in the paper.
3#The results of the study are not described in detail as to how the metabolome and proteome
were correlated and analysed, the conclusions are not detailed enough, the overall analysis is
too simple, and the images are too simple and not clear enough.
4#Proteomics and metabolomics testing companies should be described in the paper.
Review for 10.1186/s12876-024-03422-5 (2024)

Title: Integration of bile proteomics and metabolomics analyses reveals novel insights into
different types of gallstones in a high-altitude area
Version: 4 Date: 03 Sep 2024
Reviewer:  Zhanlong Mei
Reviewer's report:
Thank you for your thorough responses and revisions in response to the feedback I previously
provided. I have carefully reviewed your detailed answers and the updated manuscript. Your
revisions have significantly improved the clarity and rigor of the paper.
Review for 10.1186/s12876-024-03422-5 (2024)

Title: Integration of bile proteomics and metabolomics analyses reveals novel insights into
different types of gallstones in a high-altitude area
Version: 4 Date: 11 Sep 2024
Reviewer:  Anwar Ashraf Abouelnasr
Reviewer's report:
No further comments to add. Recommend acceptance
Review for 10.1186/s12876-024-03419-0 (2024)

Title: Gamma-glutamyl Transferase to Aspartate Aminotransferase Ratio (GSR) Predicts
Prognoses in Patients with Colorectal Cancer with Liver Metastasis after Microwave Ablation
Version: 1 Date: 21 Jun 2024
Reviewer:  Gustavo Nari
Reviewer's report:
Dear authors, I have read your paper with great interest. In my opinion, it is difficult to
understand how the GSR index, applied pre-ablation, can be a predictor of poor oncological
prognosis. You point out Xiao's work with the use of the Gamma GT, trying to harmonize it
with her results, but said work mentions an elevation of the GGT post resection while you use
it pre-ablation. It is well known that GGT and alkaline phosphatase are associated, apart from
cholestasis, to hepatic parenchymal damage due to occupying lesions, so it is logical that in
Xiao's work it is an independent predictive factor of poor prognosis.
You mention that the elevation of the index is also associated with patients with cholelithiasis,
an association that is known and that some parameters were considered within the predictive
factors of common bile duct stones, for example by Heuer-Jensen.
They state that the elevation of the GSR index could be associated with an aggressive tumor
phenotype but they have not investigated the tumor biology of these patients to be able to confirm
this.
In my opinion, the paper has many methodological weaknesses and should be strongly revised.
Review for 10.1186/s12876-024-03419-0 (2024)

Title: Gamma-glutamyl Transferase to Aspartate Aminotransferase Ratio (GSR) Predicts
Prognoses in Patients with Colorectal Cancer with Liver Metastasis after Microwave Ablation
Version: 1 Date: 25 Jun 2024
Reviewer:  Islam H. Shawali
Reviewer's report:
Review for 10.1186/s12876-024-03419-0 (2024)

Title: Gamma-glutamyl Transferase to Aspartate Aminotransferase Ratio (GSR) Predicts
Prognoses in Patients with Colorectal Cancer with Liver Metastasis after Microwave Ablation
Version: 1 Date: 25 Jun 2024
Reviewer:  Nourhan Badwei
Reviewer's report:
It's interesting data however several issues need to be carefully addressed;
_English editing with scientific terms is highly recommended
_Conclusion in the abstract and at the end of the manuscript are both subjective, kindly revise
_Kindly revise the introduction with more in-depth and updated illustrations regarding CRC and
liver Mets epidemiology and management, particularly ablative therapy, also the value of such
marker in the management
_Kindly explain the inclusion/exclusion criteria in the methods section ‘’The inclusion and
exclusion criteria, diagnostic procedures, and treatment modalities corresponded to those
explicated comprehensively within our prior study[20]’’
_Ethical consideration and declaration section are missed
Review for 10.1186/s12876-024-03419-0 (2024)

Title: Gamma-glutamyl Transferase to Aspartate Aminotransferase Ratio (GSR) Predicts
Prognoses in Patients with Colorectal Cancer with Liver Metastasis after Microwave Ablation
Version: 2 Date: 03 Aug 2024
Reviewer:  Islam H. Shawali
Reviewer's report:
Dear Authors,
Thanks you for the revised version
• The topic is discussed in a clear informative way.
Review for 10.1186/s12876-024-03419-0 (2024)

Title: Gamma-glutamyl Transferase to Aspartate Aminotransferase Ratio (GSR) Predicts
Prognoses in Patients with Colorectal Cancer with Liver Metastasis after Microwave Ablation
Version: 2 Date: 03 Aug 2024
Reviewer:  Nourhan Badwei
Reviewer's report:
The authors answered my inquiries
Review for 10.1186/s12876-024-03419-0 (2024)

Title: Gamma-glutamyl Transferase to Aspartate Aminotransferase Ratio (GSR) Predicts
Prognoses in Patients with Colorectal Cancer with Liver Metastasis after Microwave Ablation
Version: 2 Date: 13 Aug 2024
Reviewer:  Gustavo Nari
Reviewer's report:
Dear authors, I have reread the paper, and I still cannot find a rational explanation that justifies
that GSR can be a factor in poor pre-abalization oncological prognosis. Perhaps I have not
adequately understood the mechanism by which this relationship can be used as a prognostic
factor.
Review for 10.1186/s12876-024-03419-0 (2024)

Title: Gamma-glutamyl Transferase to Aspartate Aminotransferase Ratio (GSR) Predicts
Prognoses in Patients with Colorectal Cancer with Liver Metastasis after Microwave Ablation
Version: 3 Date: 04 Sep 2024
Reviewer:  Gustavo Nari
Reviewer's report:
Dear authors thank you very much for your corrections.
Review for 10.1186/s12876-024-03419-0 (2024)

Title: Gamma-glutamyl Transferase to Aspartate Aminotransferase Ratio (GSR) Predicts
Prognoses in Patients with Colorectal Cancer with Liver Metastasis after Microwave Ablation
Version: 3 Date: 13 Sep 2024
Reviewer:  Islam H. Shawali
Reviewer's report:
I find the revised version is more representative of the nice work done in this paper.
I think the results can be a milestone for further studies.
Review for 10.1186/s12876-024-03413-6 (2024)

Title: OncomiR-181a promotes carcinogenesis by repressing the extracellular matrix
proteoglycan decorin in hepatocellular carcinoma
Version: 2 Date: 31 Jul 2024
Reviewer's report:
The  manuscript  have revised carefully according to the suggestions. It looks good now.
Review for 10.1186/s12876-024-03413-6 (2024)

Title: OncomiR-181a promotes carcinogenesis by repressing the extracellular matrix
proteoglycan decorin in hepatocellular carcinoma
Version: 2 Date: 11 Aug 2024
Reviewer's report:
Several studies have found that decreased decorin (DCN) expression in HCC and its deficiency
promotes hepatic carcinogenesis (Front Oncol. 2020 May 12;10:645; Cell Signal. 2019
Oct;62:109354; Matrix Biol. 2014 Apr;35:194-205.). In this study, the authors discovered that
miR-181a targeted DCN at the 3’UTR and decreased the expression of decorin. miR-181a also
promoted cell viability, proliferation, and clonogenicity in HuH-7 cells. They also found that
miR-181a increased Myc and E2F while decreasing p53 and Rb signaling. However, there are
still some issues that need to be addressed.
1. Figure 1a shows mRNA levels of DCN in liver cancer, healthy liver tissues, and Huh7 cells.
What about the levels of miR-181a? The authors can supplement the results.
2. Figure 4c shows only a statistical graph for clonogenicity. Please display the figure under a
microscope.
3. The authors stated in their title that miR-181a promotes cell cycle progression. However,
I cannot see the direct proof in Figure 5. Please include an experiment to show that, such as
detecting with flow cytometry.
Review for 10.1186/s12876-024-03413-6 (2024)

Title: OncomiR-181a promotes carcinogenesis by repressing the extracellular matrix
proteoglycan decorin in hepatocellular carcinoma
Version: 2 Date: 12 Aug 2024
Reviewer's report:
Questions To Authors And Suggestions For Rebuttal:
1. The verification of DCN expression solely in a single cell line lacks sufficient persuasiveness
without examination in other hepatocellular carcinoma (HCC) cells. Furthermore, is there any
evidence to substantiate that the HUH7 cell line is indeed an HCV-infected cell model?
2. The methodology for selecting miR-181a should be elaborated. The relevant bioinformatic
figures and analyses should be presented to support the screening process.
3. Are there plans to investigate the effects of miR-181a in animal models to corroborate the
in vitro findings?
4. Could you clarify the potential implications of your results in the context of existing treatment
options for HCC patients?
Review for 10.1186/s12876-024-03413-6 (2024)

Title: OncomiR-181a promotes carcinogenesis by repressing the extracellular matrix
proteoglycan decorin in hepatocellular carcinoma
Version: 2 Date: 14 Aug 2024
Reviewer's report:
This work investigates miR-181a's role in liver cancer, focusing on its regulation of the
extracellular matrix proteoglycan decorin (DCN) and its impact on cell cycle progression. The
research found that DCN is significantly downregulated in liver tissues from patients with
cirrhosis and hepatocellular carcinoma (HCC) compared to healthy controls. Bioinformatics
analysis and luciferase reporter assays confirmed that miR-181a binds to the 3’UTR of
DCN. Overexpression of miR-181a in HuH-7 cells reduced DCN levels, increased cellular
viability, proliferation, and clonogenicity, and promoted cell cycle progression by modulating
key signaling pathways. These results suggest that targeting miR-181a could be a promising
therapeutic strategy for liver cancer. I recommend accepting this manuscript if the authors
address the mentioned issues.
Review for 10.1186/s12876-024-03413-6 (2024)

Title: OncomiR-181a promotes carcinogenesis by repressing the extracellular matrix
proteoglycan decorin in hepatocellular carcinoma
Version: 3 Date: 09 Sep 2024
Reviewer's report:
The authors demonstrated  that oncomiR-181a  downregulated  the expression of extracellular
matrix proteoglycan decorin in hepatocellular  carcinoma, along with a regulation on  tumor
suppressors and oncogenes. The authors have addressed the issues raised, improved the
methodology,  made accurate conclusions and  clarified the potential implications.
Review for 10.1186/s12876-024-03414-5 (2024)

Title: Efficacy and safety of direct-acting antiviral regimen for patients with hepatitis C virus
genotype 2: a systematic review and meta-analysis
Version: 1 Date: 09 Mar 2024
Reviewer's report:
I reviewed the manuscript entitled “Efficacy and safety of direct-acting antiviral regimen for
patients with hepatitis C virus genotype 2: A systematic review and meta-analysis”.
This systematic review and meta-analysis aimed to summarize the evidence on efficacy
and safety of DAAs in patients infected with HCV GT2. The authors compared DAA-
based treatments indirectly using meta-analysis and found regimens containing sofosbuvir and
velpatasvir for 12 weeks to be the relatively safe and most effective option for HCV GT2
patients.
Please find my comments:
(1) There are also limitations to this study. The population of GT2 is relatively small, therefore
the authors should be more cautious in their conclusion when they stated that regimens
containing sofosbuvir and velpatasvir for 12 weeks to be the relatively safe and most effective
option for HCV GT2 patients. Their data does not allow to conclude that sofosbuvir and
velpatasvir is better than glecaprevir penbratasvir.
(2) The data from independent clinical trials found that DAA-based treatments are safe and
effective, therefore it was obvious that a meta-analysis will find the same. What is the reason
for this paper ?
Review for 10.1186/s12876-024-03414-5 (2024)

Title: Efficacy and safety of direct-acting antiviral regimen for patients with hepatitis C virus
genotype 2: a systematic review and meta-analysis
Version: 1 Date: 15 Jun 2024
Reviewer's report:
Summary of Study on DAAs Efficacy for GT2 SVR
This study is a significant effort to compile various studies evaluating the efficacy of Direct-
Acting Antivirals (DAAs) on genotype 2 (GT2) sustained virologic response (SVR). It is well
written with very few typos.
Methods
The methods used in the study are generally appropriate and acceptable. However, there are a
few important considerations:
Duplicate Studies:
It appears that some duplicate studies, indicated by the same author ID, are included in the meta-
analysis. This could skew the results.
Recommendation: Remove duplicate studies to ensure accurate results.
Hierarchical Model Analysis:
An exploratory hierarchical model analysis should be performed if it has not been done already.
This analysis can provide more robust results by accounting for the variability between different
study levels.
Separation of Patient Groups:
The analysis should separate patients based on their prior exposure to DAAs and/or injectables/
ribavirin combinations. Specifically:
Treatment-naive patients
Treatment-experienced patients
Combined groups
This distinction can provide more precise insights into the efficacy of DAAs for different patient
groups.
Strong consideration of Network Meta-Analysis:
The authors should consider using Network Meta-Analysis (NMA) instead of traditional
meta-analysis.NMA can compare multiple treatments simultaneously and provide a more
comprehensive assessment of their relative effectiveness.
For instance, in the context of evaluating the efficacy of Direct-Acting Antivirals (DAAs)
for Hepatitis C Virus (HCV) genotype 2 (GT2) SVR, NMA can compare multiple DAA
regimens (e.g., SOF+VEL, GLE+PIB, SOF+VEL+VOX) simultaneously. Even if some of these
regimens have not been directly compared in trials, NMA can use data from studies where these
regimens were compared against a common control to estimate their relative effectiveness. This
comprehensive approach provides a clearer picture of which regimen is most effective for GT2
SVR, guiding treatment decisions and optimizing patient outcomes.
Discussion
The discussion section will depend significantly on the un-skewed results obtained after
addressing the above issues and/or the use of more advanced methods like Network Meta-
Analysis. The authors should emphasize the clinical significance of their findings to all
stakeholders, highlighting how these results can impact treatment decisions and patient
outcomes.
Review for 10.1186/s12876-024-03414-5 (2024)

Title: Efficacy and safety of direct-acting antiviral regimen for patients with hepatitis C virus
genotype 2: a systematic review and meta-analysis
Version: 4 Date: 25 Aug 2024
Reviewer's report:
The manuscript changes appear appropriate and acceptable.
I would suggest the following improvements
1. Noticed one typo - this can be easily corrected.  Kindly recheck your  whole document.
2. The conclusions should be clearly illustrated by the results  e.g.
Regime A     response rate  92 %,  ( 95%CI ;   90%, 94%
Regime  B     response rate  96 %,  ( 95%CI ;   92%, 98%
Regime  C     response rate  94 %,  ( 95%CI ;   92%, 96%
if this is in appendix,  i would bring it forward  and  have the meta analysis of all studies transfered
to appendix  only providing  a summary of the forrest plot in  text.  That is viable only if you
have enough numbers.
3.   Figure 3 and table 3 needs to be explained more. it is important to know that many readers
may not be conversant with Network Diagrams.  i suggest that this could be explained better  e.g.,
“Figure 3  illustrates the network of studies included in our network meta-analysis, comparing
five different  regimes . The nodes represent each regime , with the size of each node
corresponding to the number of patients included in studies of that treatment. The edges between
nodes indicate direct comparisons made in at least one study, with thicker edges representing
comparisons evaluated in multiple studies. Notably, all regimes were compared directly to at
least one  regime, forming a well-connected network. However, comparisons between regime B
and  regime C  rely primarily on indirect evidence, as indicated by the thinner edge connecting
these nodes. The assessment of inconsistency showed no significant differences between direct
and indirect comparisons within this network.”
My  summary of these results would be : since YYYY several regimes  have been used for
treatment of GT2 with a   response  of  96% , 95%CI  92%, 98%   for those treated  8-12
months,.....
Regimes B and C  appeared to  demonstrate the highest response rates  compared to other regimes
(use confidence intervals)
Review for 10.1186/s12876-024-03431-4 (2024)

Title: Diagnosing celiac disease in children using oral manifestations
Version: 3 Date: 20 Aug 2024
Reviewer's report:
The manuscript presents an interesting investigation into the diagnosis of Celiac Disease (CD)
based on RAS and MIH. I think that it can contribute to understand more about the potential
role of oral manifestations in diagnosis, however some part need to be addressed to provide a
more detailed and precise overview of the study's finding and implications, as well as correct
some typos and grammatical issues that should be refined for clarity. Furthermore the structure
of the manuscript should be improved
1. Abstract
- the statistical analysis should be clarified better
- Provide a more specific conclusion, emphasizing the potential of MIH as a marker for CD
2. Material and Methods
- It would be useful to add an experimental design of the clinical study in which is reported
the recruitment stage; the screening of the participants with the number of participant that were
considered eligible and the number of participants for the subgroups; the analysis performed;
maybe the length of the study; the outcomes
- Inclusion and exclusion criteria can be better reported in a table...as inclusion or exclusion
criteria you could also add the age and the gender should be specified
- Give more information about the questionnaire used ...unless it was made of only two questions
- The statistical analysis need a specific subsection in material and methods
- More information about the reference used for the decision of the sample size should be added
or better explained
3. Results
- The demographic table can be moved in the material and method section and I would change
boy/girl with male/female in the gender row
- I suggest to write the data regarding the intensity and distribution of MIH lesion in a table
4. Discussion & Conclusion
- Several typos need to be correct e.g. demonsrated to demonstrated or a . where there should be
a comma. Unfortunately I can't be more specific as line numbers are missing in this manuscript
- My main concern is the weakness of the discussion and the clarity of the conclusion. Sometimes
it looks as repetition of the introduction. To strengthen the discussion, it would be beneficial to
delve deeper into how these findings align with or challenge existing literature, and to clarify
the clinical significance of the results.
The discussion emphasizes the potential role of dentists in diagnosing CD based on oral
manifestations, however the study's small sample size and in particular that only two children
were diagnosed with CD, should be highlighted more clearly as a limitation, suggesting caution
in generalizing the findings.
The NCGS adds an important information, but the relationship between NCGS and enamel
defects is only briefly touched.
You might expand even more about the role and findings related to the control group and in the
conclusion provide a clearer path forward suggesting which other oral manifestations might also
be worth investigating in relation to CD or highlighting the importance of a multidisciplinary
approach in diagnosis CD
Review for 10.1186/s12876-024-03431-4 (2024)

Title: Diagnosing celiac disease in children using oral manifestations
Version: 4 Date: 18 Sep 2024
Reviewer's report:
I would like to thank the authors for their thorough and thoughtful revisions. They have
addressed all my concerns and significantly improved the manuscript. I have no further
comments or suggestions, and I recommend the manuscript for publication in its current form
Review for 10.1186/s12876-024-03423-4 (2024)

Title: Prolonged Video Capsule Endoscopy Examination Durations Can Improve Capsule
Endoscopy Completeness
Version: 2 Date: 01 Jun 2024
Reviewer's report:
While CE time extension is a good idea, I think that this paper does not fully meet the clinical
value as an original paper according to the limitations as below.
1. How did authors manage gastric transit? They checked on the way?  How was GTT?
2. CE time extension may lead the adverse events related to procedure such as device damage.
please describe and discuss the issue.
3. participants was about 80. So logistic regression analysis should be performed by only 7-8
factors having the impact on the outcome.
Review for 10.1186/s12876-024-03423-4 (2024)

Title: Prolonged Video Capsule Endoscopy Examination Durations Can Improve Capsule
Endoscopy Completeness
Version: 2 Date: 27 Jun 2024
Reviewer's report:
Not original but again interesting issue
The study design is well done and the study group consistent
Some arguments to be focused on the discussion:
are there comparison study between different type of capsule  regarding completeness of the
transit and the transit speed??
Discuss the opportunity with small capsules to be launched , if there are data on this new type
of capsules regarding speed and transit time
Review for 10.1186/s12876-024-03423-4 (2024)

Title: Prolonged Video Capsule Endoscopy Examination Durations Can Improve Capsule
Endoscopy Completeness
Version: 2 Date: 16 Jul 2024
Reviewer's report:
Thank you for your work in this area. My suggestions are as follows:
1. Could you add any information about diagnostic yield of multi-phasic CT enterography  in
obscure GI bleeding in the introduction?
2. Line 83 - PillCam is miss spelled.
3. In the methods section, you mentioned bowel prep in the protocol but in results section it
states on 50% took bowel prep. Please explain.
4. Line 205 - typo  - 8 hours?
I agree with your limitations.
Review for 10.1186/s12876-024-03423-4 (2024)

Title: Prolonged Video Capsule Endoscopy Examination Durations Can Improve Capsule
Endoscopy Completeness
Version: 3 Date: 06 Aug 2024
Reviewer's report:
The current version remains suboptimal for publication. The study has a certain number of
inherit limitations, the above is suggested as improvements. The Liao paper from 2010! should
be replaced with the more recent:  Cortegoso Valdivia P, Skonieczna-#ydecka K, Elosua A, et al.
Indications, Detection, Completion and Retention Rates of Capsule Endoscopy in Two Decades
of Use: A Systematic Review and Meta-Analysis. Diagnostics (Basel). 2022 Apr 28;12(5):1105.
doi: 10.3390/diagnostics12051105.
The systematic review of the CE and enteroscopy should be replaced with Wang Z, Chen
JQ, Liu JL, Qin XG, Huang Y. CT enterography in obscure gastrointestinal bleeding: a
systematic review and meta-analysis. J Med Imaging Radiat Oncol. 2013 Jun;57(3):263-73. doi:
10.1111/1754-9485.12035.
Review for 10.1186/s12876-024-03427-0 (2024)

Title: Effect of Skeletal Muscle Index on Post-embolization Syndrome of Hepatocellular
Carcinoma after Transarterial Chemoembolization
Version: 3 Date: 16 Jul 2024
Reviewer's report:
This paper is written about factors associated with post TACE embolization syndrome. This is
imprtant in clinical practice.  However,  you  should clarify if  the therapeutic effect with TACE
was associated with SMI. If it was not relevant, you should write in the paper.
Review for 10.1186/s12876-024-03427-0 (2024)

Title: Effect of Skeletal Muscle Index on Post-embolization Syndrome of Hepatocellular
Carcinoma after Transarterial Chemoembolization
Version: 3 Date: 20 Jul 2024
Reviewer's report:
This manuscript showed a possible relationship between low SMI and the development of PES.
I have some suggestions.
1. The prevalence of PES was relatively high in this study. The detailed symptoms or signs for
the diagnosis of PES should be described (i.e., fever, the severity of pain, CRP levels). PES might
be further stratified according to the severity of symptoms, and I suggest additional analyses
based on this stratification.
2. Furthermore, the manuscript does not discuss a possible mechanism for the relationship
between low SMI and the development of PES. This should be addressed in the discussion
section.
Review for 10.1186/s12876-024-03427-0 (2024)

Title: Effect of Skeletal Muscle Index on Post-embolization Syndrome of Hepatocellular
Carcinoma after Transarterial Chemoembolization
Version: 3 Date: 27 Jul 2024
Reviewer:  Islam H. Shawali
Reviewer's report:
This retrospective Study presents very important results regarding the prognosis of post-TACE
for HCC post embolization syndrome based upon the effect of the skeletal muscle index.
The authors handled the approach in a very good way and presented their experiences from their
health center in a clear  manner.
I consider this work to have a contribution to the pre-treatment preparation, I have no objections,
and I highly recommend it for publication.
Review for 10.1186/s12876-024-03427-0 (2024)

Title: Effect of Skeletal Muscle Index on Post-embolization Syndrome of Hepatocellular
Carcinoma after Transarterial Chemoembolization
Version: 3 Date: 03 Aug 2024
Reviewer's report:
1- Abstract: What about the response to TACE in those patients (mRecist criteria) and relation
to PES?
2- Introduction: Needs English editing.
3- Methods&Results
# What about the tumor differentiation according to histopathology?
# Response to TACE and m-Recist criteria and relation to the skeletal muscle index and PES
# The authors did not mention the fate of those patients with PES
( Treatment , Hospital admissions ….)
4- Discussion:
# It is better to be modified.
5- References:
More recent references better to be added.
Review for 10.1186/s12876-024-03427-0 (2024)

Title: Effect of Skeletal Muscle Index on Post-embolization Syndrome of Hepatocellular
Carcinoma after Transarterial Chemoembolization
Version: 6 Date: 30 Aug 2024
Reviewer's report:
The manuscript is a well-conducted retrospective analysis with appropriate methodology and
a clear presentation of results. However, I recommend further discussing the discrepancies
observed in Table 2 between univariate and multivariate analyses, for example diabetes and
serum albumin. I would need clarification on the statistical model selection and a deeper
exploration of the clinical implications of the findings. Strengths, limitations, and ethical
considerations should be more thoroughly addressed.
Review for 10.1186/s12876-024-03427-0 (2024)

Title: Effect of Skeletal Muscle Index on Post-embolization Syndrome of Hepatocellular
Carcinoma after Transarterial Chemoembolization
Version: 6 Date: 30 Aug 2024
Reviewer's report:
The questions  and suggestions i have mentioned have been answered as possible, i have no
further recommendation to this article.