Review for 10.1186/s12876-022-02208-x (2022)

Reviewer’s report
Title: 
RIP3 knockdown inhibits necroptosis of human intestinal epithelial cells
via TLR4/MyD88/NF-κB signaling and ameliorates murine colitis
Version: 
1 
Date: 
25 Feb 2022
Reviewer's report:
Reviewer 2 Revision comments:
1. New supplemental figure 2A: There is no observable difference between
WT+DSS vs WT+DSS+NEC1 however there is a 2-star significance listed. Are
these groups statistically different?
2. There is no statistical significance listed on supplemental figure 1b. Are the
groups different? If they are not different this panel and corresponding western
blot should be removed.
3. The new supplemental figures are not supplemental; they should be in main
figures.
4. The authors should cite in either the results (preferred) or the discussion the
other manuscripts that had already tested the RIP3 KO mice and Nec-1 treated
mice response to DSS. (Reviewer 2 comment #1)
5. reviewer 3, comment #4: 4D WT size does not seem to have changed. It does
appear smaller than the rest of the other panels.
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