Review for 10.1186/s12876-021-01722-8 (2021)

Reviewer’s report
Title: 
Increased V
δ
1 
γδ
T cells Predominantly Contributed to IL-17 Production
in the Development of Adult Human Post-infectious Irritable Bowel Syndrome
Version: 
1 
Date: 
19 Jun 2020
Reviewer's report:
The current manuscript examines an interesting hypothesis, i.e. the role of V
δ
1
γδT cells in PI-IBS.
There are several issues which need to be addressed. 
1) Just asking about the history of infection is not sufficient to make the
diagnosis of PI-IBS. Many patients may admit having GI infection in the past
and many of them may not have IBS. A better documentation is necessary.
2) The methodology is not clear and it is not mentioned how many of the patients
and controls had Increased Vδ1 γδT cells. 
3) Also, the IHC data needs better quantification. 
4) The authors have mixed the methods and results at some points and I rarely
see any P-Value in this manuscript.
Overall, it is difficult to read this manuscript. 
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No
Are the methods appropriate and well described?
If not, please specify what is required in your comments to the authors.
No
Does the work include the necessary controls?
If not, please specify which controls are required in your comments to the
authors.
No
Are the conclusions drawn adequately supported by the data shown?
If not, please explain in your comments to the authors.
No
Are you able to assess any statistics in the manuscript or would you
recommend an additional statistical review?
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require further assessment in your comments to the editors.
I am able to assess the statistics
Quality of written English
Please indicate the quality of language in the manuscript:
Needs some language corrections before being published
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. 
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