Review for 10.1186/s12876-021-01707-7 (2021)

Reviewer’s report
Title: 
Gene Expression Profiling of Inflammatory Cytokines in Esophageal
Biopsies of Different Phenotypes of Gastroesophageal Reflux Disease: a cross-
sectional study
Version: 
3 
Date: 
19 Feb 2021
Reviewer's report:
PEER REVIEWER ASSESSMENTS: 
OBJECTIVE - Full research articles: is there a clear objective that addresses one
or several testable research questions? (Brief or other article types: is there a
clear objective?)
Yes - there is a clear objective
DESIGN - Is the current approach (including controls and analysis protocols)
appropriate for the objective?
Yes - the approach is appropriate
EXECUTION - Are the experiments and analyses performed with sufficient
technical rigor to allow confidence in the results?
Yes - experiments and analyses were performed appropriately
STATISTICS - Is the use of statistics in the manuscript appropriate?
Yes - appropriate statistical analyses have been used in the study
INTERPRETATION - Is the current interpretation/discussion of the results
reasonable and not overstated?
Yes - the author's interpretation is reasonable
OVERALL MANUSCRIPT POTENTIAL - Has the author addressed your
concerns sufficiently for you to now recommend the work as a technically sound
contribution? If not, can further revisions be made to make the work technically
sound?
Yes - current version is technically sound
PEER REVIEWER COMMENTS: 
GENERAL COMMENTS: The authors have adequately answered the questions
of the reviewer and greatly improved the manuscript. They have especially
worked on the graphs, so that the data would be easier to understand by the
reader. They have also included some corrections of terminology, and modified
the information given on tables. Overall the manuscript has a higher quality and
it is deemed suitable for publication.
REQUESTED REVISIONS: 
Figure 1 contains all the information from figures 2, 3 and 4. Considering
specifying that those figures originate from number 1, or that 1 is a global
comparison of the data in 2, 3 and 4.
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Declaration of competing interests
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questions:
1
. 
Have you in the past five years received reimbursements, fees, funding, or
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Have you received reimbursements, fees, funding, or salary from an
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Do you have any non-financial competing interests in relation to this
paper?
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recommendation.
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Is the study design appropriate to answer the research question (including the use
of appropriate controls), and are the conclusions supported by the evidence
presented?
Yes
Are the methods sufficiently described to allow the study to be repeated?
Yes
Is the use of statistics and treatment of uncertainties appropriate?
Yes
Is the presentation of the work clear?
Yes
Are the images in this manuscript (including electrophoretic gels and blots) free
from apparent manipulation?
Yes