Review for 10.1186/S12876-019-1048-3 (2019)

Reviewer ’s report  
 
Title: The contribution of miR -122 to the innate immunity by regulating Toll -like receptor 4 in 
hepatoma cells  
 
Version: 3 Date: 06 Jun 2019  
 
Reviewer: Jharna Datta  
Reviewer's report:  
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In this manuscript entitled "The contribution of miR -122 to the innate immunity by regulating 
Toll-like receptor 4 in hepatoma cells" by Shi et. al., the authors have performed  extensive 
studies on the tumor suppressor role of miR -122 in innate immunity by regulating Toll -like 
receptor 4 (TLR4) in hepatocellular carcinoma (HCC).  The authors through mutational studies 
in 3'-UTR region of TLR4, and ectopic expression /knocking do wn of miR -122 in hepatoma 
cells have shown that miR -122 directly targets TLR4 and thereby play an important role in 
proliferation of HCC cells by modulating inflammatory pathways. This study also signifies that 
immune modulation through targeting TLR4 by m iR-122 could be a potential therapeutic 
strategy for HCC treatment.  
Are the methods appropriate and well described?  
If not, please specify what is required in your comments to the authors.  
 
Yes 
 
Does the work include the necessary controls?  
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Yes 
 
Are the conclusions drawn adequately supported by the da ta shown?  
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Yes 
 
  
Are you able to assess any statistics in the manuscript or would you recommend an 
additional statistical review?  
If an additional statistical review is recommended, please specify what  aspects require further 
assessment in your comments to the editors.  
 
Not relevant to this manuscript  
 
Quality of written English  
Please indicate the quality of language in the manuscript:  
 
Acceptable  
 
Declaration of competing interests  
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