Review for 10.1186/s12876-021-01643-6 (2021)

Reviewer’s report
Title: 
Expression and Prognostic Impact of FZDs in Pancreatic Adenocarcinoma
Version: 
1 
Date: 
07 Jan 2021
Reviewer's report:
The authors studied the role of FZDs in pancreatic cancer using multiple public
database, have revised the manuscript, and now concluded that FZD3 and FZD6
could be potential prognostic and predictive markers for survival of patients with
pancreatic adenocarcinoma.
1. Although the authors show only multivariable analysis for OS and RFS in
Table 1, univariable analysis should also be shown first.
2. The reviewer does not find any description of risk analysis for survival, which
should be described in the revised manuscript.
3. The authors described in abstract as follows: The RFS difference between low
FZD6 expression group and high expression group was almost statistically
significant (p = 0.067). However, multivariable analysis showed that FZD6 was
the only independent factor for RFS. In general, p value of 0.067 indicates being
"not significant" and they should mention why this variable was considered in
multivariable analysis.
4. The resolution of the majority of figures submitted is still very low. This
problem interferes the reviewer to judge if the interpretations of those study
results are appropriate or not.
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Is the study design appropriate to answer the research question (including the use
of appropriate controls), and are the conclusions supported by the evidence
presented?
No
Are the methods sufficiently described to allow the study to be repeated?
No
Is the use of statistics and treatment of uncertainties appropriate?
No
Is the presentation of the work clear?
No
Are the images in this manuscript (including electrophoretic gels and blots) free
from apparent manipulation?
No