Review for 10.1186/s12876-020-01532-4 (2021)

Reviewer’s report
Title: 
Overexpression of P-glycoprotein, MRP2, and CYP3A4 impairs intestinal
absorption of octreotide in rats with portal hypertension
Version: 
3 
Date: 
19 Jun 2020
Reviewer's report:
-> ARRIVE guidelines require to provide details on animal sex and age. (See
Item #8) This is missing.
-> ARRIVE guidelines require information about animal numbers (see Item #10
and #11). You answer this in the response letter "In our study, 80 rats have been
randomly selected from 100 rats to build PH model of rats. By model
verification, 57 PH rats have been established successfully. Then, 48 rats form
those PH rats were randomly selected and were divided into respective PH
experimental groups at random (n=4 or 6). In addition, other residue PH rats
were preserved and reserved in our study." - but this information is not presented
in the methods part of the manuscript. A study flow chart (as a supplementary
figure) might be helpful to understand your approach to select 48 rats out of 100
(!) animals. Moreover a definition of "model verification" is missing.
-> Please revise the language/grammar of the sentence: "Moreover, after PH rats
were operated, they still have been applied anesthesia with ether for 24 hours
every one hour in order to alleviate postoperative pain and abnormal
phenomenon of them were observed."
-> 
Please revise the language/grammar of the sentence: "The all animal
experiments were performed after PH model of rats were established
successfully and there were not any drop-out during anaesthetization or
retrospective experiments."
-> The supplementary Western Blot images are poorly illustrated and labelled.
Why is only one band highlighted per group? What about the other bands?
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Are the methods appropriate and well described?
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Yes
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authors.
Yes
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Yes
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I am able to assess the statistics
Quality of written English
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Needs some language corrections before being published
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