Review for 10.1186/s12876-020-01331-x (2020)

Reviewer’ s report
Title: Prevalence of non Helicobacter pylori gastric Helicobacters in Iranian dyspeptic patients
Version: 2Date: 10 Oct 2019
Reviewer: Research Square Reviewer 1
Reviewer's report:
PEER REVIEWER ASSESSMENTS: OBJECTIVE - Full research articles: is there a clear
objective that addresses one or several testable research questions? (Brief or other article types: isthere a clear objective?)Yes - there is a clear objectiveDESIGN - Is the current approach (includingcontrols and analysis protocols) appropriate for the objective?Yes - the approach isappropriateEXECUTION - Are the experiments and analyses performed with sufficient technicalrigor to allow confidence in the results?Yes - experiments and analyses were performedappropriatelySTATISTICS - Is the use of statistics in the manuscript appropriate?Yes - appropriatestatistical analyses have been used in the studyINTERPRETATION - Is the current interpretation/discussion of the results reasonable and not overstated?Yes - the author's interpretation isreasonableOVERALL MANUSCRIPT POTENTIAL - Has the author addressed your concernssufficiently for you to now recommend the work as a technically sound contribution? If not, canfurther revisions be made to make the work technically sound?Yes - current version is technicallysoundPEER REVIEWER COMMENTS: GENERAL COMMENTS: The authors have respondedto most of the technical concerns that this reviewer had. The authors have provided a gel picture(Figure 1) that clearly shows how they differentiated between Helicobacter species based onamplicon size. This figure could be submitted as a supplementary figure and does not necessarilyhave to be part of the main text. The authors have clarified the use of the Real-Time PCR.
Although somewhat improved, the English, syntax and style of the manuscript is still
poor.ADDITIONAL REQUESTS/SUGGESTIONS: The language, sythax and style still needsattention.
Are the methods appropriate and well described?
If not, please specify what is required in your comments to the authors.
YesDoes the work include the necessary controls?
If not, please specify which controls are required in your comments to the authors.
YesAre the conclusions drawn adequately supported by the data shown?
If not, please explain in your comments to the authors.
Yes
Are you able to assess any statistics in the manuscript or would you recommend an
additional statistical review?If an additional statistical review is recommended, please specify what aspects require furtherassessment in your comments to the editors.
I am able to assess the statisticsQuality of written English
Please indicate the quality of language in the manuscript:
Needs some language corrections before being published
Declaration of competing interests
Please complete a declaration of competing interests, considering the following questions:
1. Have you in the past five years received reimbursements, fees, funding, or salary from an
organisation that may in any way gain or lose financially from the publication of thismanuscript, either now or in the future?
2. Do you hold any stocks or shares in an organisation that may in any way gain or lose
financially from the publication of this manuscript, either now or in the future?
3. Do you hold or are you currently applying for any patents relating to the content of the
manuscript?
4. Have you received reimbursements, fees, funding, or salary from an organization that holds
or has applied for patents relating to the content of the manuscript?
5. Do you have any other financial competing interests?
6. Do you have any non-financial competing interests in relation to this paper?
If you can answer no to all of the above, write 'I declare that I have no competing interests' below.
If your reply is yes to any, please give details below.
This reviewer has been recruited by a partner organization, Research Square. Reviewers with
declared or apparent competing interests are not utilized for these reviews. This reviewer hasagreed to publication of their comments online under a Creative Commons Attribution Licenseattributed to Research Square and was paid a small honorarium for completing the review within aspecified timeframe. Honoraria for reviews such as this are paid regardless of the reviewerrecommendation.
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on my report to the authors and, if the manuscript is accepted for publication, my named reportincluding any attachments I upload will be posted on the website along with the authors'
responses. I agree for my report to be made available under an Open Access Creative Commons
CC-BY license (http://creativecommons.org/licenses/by/4.0/). I understand that any comments
which I do not wish to be included in my named report can be included as confidential commentsto the editors, which will not be published.
I agree to the open peer review policy of the journal