Review for 10.1186/s12876-017-0634-5 (2017)

Reviewer ’s report  
 
Title: Role of serotonin on the intestinal mucosal immune response to stress -induced diarrhea in  
weaning mice  
 
Version: 0 Date: 20 Apr 2016  
 
Reviewer: Nan Wu  
 
Reviewer's report:  
 
The authors have revised the manuscript according to the reviewer's comments on the first 
submiss ion and improved the manuscript greatly. However, the following points should be 
concerned by the authors before publication.  
 
 
 
 
1. The tense used in "statistical analysis" on Page 8 is not consistent.  
 
 
2. What's the unit of vertical axis in figure 3B and 3C?  
 
 
3. The font and size of subtitles in the manuscript should be consistent.  
 
 
4. The expression of CD3+CD4+ and CD3+CD8+ should be consistent in the manuscript. 
Sometimes they are expressed as CD3+CD4+ and CD3+CD8+.  
 
 
5. In figures, the font and size of labels for "A", "B" and "C" are not consistent.  
 
 
6. The authors have descripted 204 mice used in this study. But they didn't describe how 
many mice were used in each group.  
 
 
7. In figure 4A, did the authors make sure the difference among groups were significant? It  
looks like no significant differences among groups according to the figure.  
 
 
8. In discussion, there is no need to use subtitles.  
 
 
 
Are the methods appropriate and well described?  
If not, please specify what is required in your comments to the authors.  
 
Yes 
 
Does the work include the necessary controls?  
If not, please specify which controls are required in your comments to the authors.  
 
Yes 
 
Are the conclusions drawn adequately supported by the data shown?  
If not, please explain in your comments to the authors.  
 
Yes 
 
Are you able to assess any statistics in t he manuscript or would you recommend an  
additional statistical review?  
If an additional statistical review is recommended, please specify what aspects require further  
assessment in your comments to the editors.  
 
I am able to assess the statistics  
 
Quality of  written English  
Please indicate the quality of language in the manuscript:  
 
Needs some language corrections before being published  
 
Declaration of competing interests  
Please complete a declaration of competing interests, considering the following questions:  
 
1. Have you in the past five years received reimbursements, fees, funding, or salary from an  
organisation that may in any way gain or lose financially from the publication of this  
manuscript, either now or in the future?  
 
2. Do you hold any stocks or share s in an organisation that may in any way gain or lose  
financially from the publication of this manuscript, either now or in the future?  
 
3. Do you hold or are you currently applying for any patents relating to the content of the  
manuscript?  
 
4. Have you received reimbursements, fees, funding, or salary from an organization that 
holds  or has applied for patents relating to the content of the manuscript?  
 
5. Do you have any other financial competing interests?  
 
6. Do you have any non -financial competing inter ests in relation to this paper?  
If you can answer no to all of the above, write 'I declare that I have no competing interests' 
below.  If your reply is yes to any, please give details below.  
 
I declare that I have no competing interests' below.  
 
I agree to the open peer review policy of the journal. I understand that my name will be included  
on my report to the authors and, if the manuscript is accepted for publication, my named report  
including any attachments I upload will be posted on the website along with the au thors'  
responses. I agree for my report to be made available under an Open Access Creative Commons  
CC-BY license ( http://creativecommons.org/licenses/by/4.0/ ). I understand that any comments  
which I do not wish to be included in my named report can be incl uded as confidential comments  
to the editors, which will not be published.  
 
I agree to the open peer review policy of the journal