Review for 10.1186/s12876-019-1089-7 (2019)

Reviewer ’s report  
 
Title: Histological, immunohistochemical and mRNA gene expression responses in coeliac  
disease patients challenged with gluten using PAXgene fixed paraffin -embedded duodenal  
biopsies  
 
Version: 1 Date: 17 Sep 2019  
 
Reviewer: Rong Jun Guo  
 
Reviewer's report:  
 
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The authors address my concerns, but the response to my 1st point is not satisfactory: ' In our 
experience the yield of RNA extracted from FFPE blocks is much lower than from Paxgene 
fixed blocks".  They should show data to support their statement.  The re ason I think is important 
is that FFPE is so commonly used in clinical laboratories. Until you have compelling data to 
show FFPE is not a good choice, readers would always ask how you know FFPE does not work.  
 
Are the methods appropriate and well described?  
If not, please specify what is required in your comments to the authors.  
 
Yes 
 
Does the work include the necessary controls?  
If not, please specify which controls are required in your comments to the authors.  
 
Yes 
 
Are the conclusions drawn adequately supported by the data shown?  
If not, please explain in your comments to the authors.  
 
Yes 
 
Are you able to assess any statistics in the manuscript or would you recommend an  
additional statistical review?  
If an additional statistical review is recommended, please specify what aspects require further  
assessment in your comments to the editors.  
 
I recommend additional statistical review  
 
Quality of written English  
Please indicate the quality of language in the manuscript:  
 
Acceptable  
 
Declaration of competing interests  
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