Review for 10.1186/s12876-020-01555-x (2020)

Reviewer’s report
Title: 
Genistein attenuated gastric inflammation and apoptosis in Helicobacter
pylori-induced gastropathy in rats
Version: 
3 
Date: 
28 Oct 2020
Reviewer's report:
This animal study aimed to evaluate the anti-inflammatory effects of genistein in
rats with H. pylori infection, and concluded that genistein exerted its protective
effects in rats with H. pylori-induced gastropathy through the reduction in NF-
kB activation, pro-inflammatory cytokine production, and gastric apoptotic cell
death.
Minor comments:
1. In the Methods (page 5, line 1-2 and 16-23), the authors described how to use
the genistein in the group-(2) and group-(3). As the authors mentioned: in group-
(2), the rats were treated with 0.1% DMSO (1ml/rat); and, in group-(3), the rats
were treated with genistein (16 mg/kg BW). There was an ambiguous
information of detailed genistein preparation and dosage in group-(3), pure
genistein gavage or "genistein xx mg/kg dissolved in 0.1% DMSO for gavage"?
2. Please update citation references associated with the anti-inflammatory effect
of genistein in gastropathy. (such as: Pharmacogn Mag. 2017 Jul;13(Suppl
2):S306-S310. 
Can J Physiol Pharmacol. 2018 Nov;96(11):1161-1170. etc.)
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