Review for 10.1186/s12876-020-01596-2 (2021)

Reviewer’s report
Title: 
Alterations in MicroRNA Gene Expression Profile in Liver Transplant
Patients with Hepatocellular Carcinoma
Version: 
1 
Date: 
25 Aug 2020
Reviewer's report:
The overall design of this paper is reasonable. Several comments are considered.
Comments:
1.The sentence of "All of HCC patients' tumors were in stage III and IV." should
illustrate the staging system, AJCC or BCLC
2.The sentence of "The sampling time of both acute rejected and nonrejected
were the same." should make it clear when the samples were obtained.
3.Could you provide the sequences of miRNA primers involved in qRT-PCR?
4.In the figure 3, it is commonly recommended that red color represents an
expression level higher than the reference and green color below the reference. 
5.It would be more meaningful if the authors provide references of papers which
adopted LNA-array method. 
6.The novelty of this work is not well highlighted. In addition, limitations of the
work and work prospects were not provided. Could you give some discussions?
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Is the study design appropriate to answer the research question (including the use
of appropriate controls), and are the conclusions supported by the evidence
presented?
Yes
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Yes
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from apparent manipulation?
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