Review for 10.1186/s12876-021-01626-7 (2021)

Reviewer’s report
Title: 
Identification of Potential Biomarkers for Abdominal Pain in IBS Patients
by Bioinformatics Approach
Version: 
1 
Date: 
09 Dec 2020
Reviewer's report:
Authors replied point by point and clarified most of the queries.
However, in the final manuscript the exact number of cases studied is not
mentioned (53 IBS and 40 HV: number of biopsies or cases?). Given the strong
variability in IBS population the number of patients is very relevant. 
Therefore they should specify in the manuscript how many cases of IBS and how
many HV were studied.
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Is the study design appropriate to answer the research question (including the use
of appropriate controls), and are the conclusions supported by the evidence
presented?
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