Review for 10.1186/s12876-018-0850-7 (2018)

Reviewer ’s report  
 
Title: Mesenchymal Stem Cell Transplantation Improves Chronic Colitis -Associated 
Complications through Inhibiting the Activity of Toll -Like Receptor -4 in Mice  
 
Version: 2 Date: 04 Jun 2018  
 
Reviewer: Dc de Almeida  
 
Reviewer's report:  
 
1) Results, Fig1. The hUC -MSCs was not explored for thei r multilineage differentiation, Please, 
inform this in the text, and show the reference where these cells were previously full 
characterized by the group or others, this information is essential to be added.  
 
2) Results. Fig5 and 6. Describe mRNA expression  of inflammatory molecules before their 
protein expression. This modification was made in the text, but not in the figures. For 
example, Figure 5E is mentioned in the text before Figure 5A, B, and this should be adjusted.  
Are the methods appropri ate and well described?  
If not, please specify what is required in your comments to the authors.  
 
Yes 
 
Does the work include the necessary controls?  
If not, please specify which controls are required in your comments to the authors.  
 
Yes 
 
Are the conclusions drawn adequately supported by the data shown?  
If not, please explain in your comments to the authors.  
 
Yes 
 
Are you able to assess any statistics in the manuscript or would you recommend an  
additional statistical review?  
If an additional statistical review is recommended, please specify what aspects require further  
assessment in your comments to the editors.  
 
I am able to assess the statistics  
 
Quality of written English  
Please indicate the quality of language in the manuscript:  
 
Needs some language corrections before being published  
 
Declaration of competing interests  
Please complete a declaration of competing interests, considering the following questions:  
 
1. Have you in the past five years received reimbursements, fees, funding, or salary from an  
organisation that may in any way gain or lose financially from the publication of this  
manuscript, either now or in the future?  
 
2. Do you hold any stocks or shares in an organisation that may in any way gain or lose  
financially from the p ublication of this manuscript, either now or in the future?  
 
3. Do you hold or are you currently applying for any patents relating to the content of the  
manuscript?  
 
4. Have you received reimbursements, fees, funding, or salary from an organization that 
holds or has applied for patents relating to the content of the manuscript?  
 
5. Do you have any other financial competing interests?  
 
6. Do you have any non -financial competing interests in relation to this paper?  
 
If you can answer no to all of the above, write 'I declare that I have no competing interests' 
below.  If your reply is yes to any, please give details below.  
 
I declare that I have no competing interests  
 
I agree to the open peer review policy of the journal. I understand that my name will be included  
on my report to the authors and, if the manuscript is accepted for publication, my named report  
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I agree to the open peer review policy of the journal