Review for 10.1186/s12876-019-0957-5 (2019)

Reviewer ’s report  
 
Title: In-depth characterization of the Wnt -signaling/ β-catenin pathway in an i n vitro model of 
Barrett's sequence  
 
Version:  1 Date: 28 Dec 2018  
 
Reviewer: Giuseppe Fanelli  
 
Reviewer's report:  
 
Despite some remarkable limitations like the human sample number used to confirm in vitro's 
data, the paper is of interest and could be a good starting point for further studies. My only 
concern is about  the number of the FFPE samples: after the revision an explanation of these 
numbers is present only in Immunoistochemistry figure legend (page 26) and not in M&M 
section (page 7), as answered to the revisor, or in other section.  
 
 
 
Are the methods appropriate and well described?  
If not, please specify what is required in your comments to the authors.  
 
Yes 
 
Does the work include the necessary controls?  
If not, please specify which controls are required in your comments to the authors.  
 
Yes 
 
Are the conclusions drawn adequately supported by the data shown?  
If not, please explain in your comments to the authors.  
 
Yes 
 
Are you able to assess any statistics in the manus cript or would you recommend an 
additional statistical review?  
If an additional statistical review is recommended, please specify what aspects requ ire further 
assessment in your comments to the editors.  
 
I am able to assess the statistics  
 
Quality of written English  
Please indicate the quality of language in the manuscript:  
 
Acceptable  
 
Declaration of competing interests  
Please complete a declaration of competing inter ests, considering the following questions:  
 
1. Have you in the past five years received reimbursements, f ees, funding, or salary from an 
organisation that may in any way gain or lose financial ly from the publication of this 
manuscript, either now or in the future?  
 
2. Do you hold any stocks or shares in an organisation t hat may in any way gain or lose 
financially from the publication of this manuscript, either now or in the future?  
 
3. Do you hold or are you currently applying for any patents relating to the c ontent of the 
manuscript?  
 
4. Have you received reimbursements, fees, funding, or salary from an organization that 
holds or has applied for patents relating to the content of the manuscript?  
 
5. Do you have any other financial competing interests?  
 
6. Do you have any non -financial competing interests in relation to this paper?  
 
If you can answer no to all of the above, write 'I declare that I have  no competing interests' 
below. If your reply is yes to any, please give details below.  
 
I declare that I have no competing interests  
 
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on my report to the authors and, if the manuscript is accepted f or publication, my named report 
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to the editor s, which will not be published.  
 
I agree to the open peer review policy of the journal