Review for 10.1186/s12876-023-02798-0 (2023)

Reviewer’s report
Title: 
Peripheral CD4+CD25hiCD127low regulatory T cells are increased in
patients with gastrointestinal cancer
Version: 
0 
Date: 
20 Jan 2023
Reviewer's report:
PEER REVIEWER ASSESSMENTS: 
OBJECTIVE - Full research articles: is there a clear objective that addresses a
testable research question(s) (brief or other article types: is there a clear
objective)?
Yes - there is a clear objective
DESIGN - Is the current approach (including controls and analysis protocols)
appropriate for the objective?
Yes - the approach is appropriate
EXECUTION - Are the experiments and analyses performed with technical rigor
to allow confidence in the results?
Yes - experiments and analyses were performed appropriately
STATISTICS - Is the use of statistics in the manuscript appropriate?
Yes - appropriate statistical analyses have been used in the study
INTERPRETATION - Is the current interpretation/discussion of the results
reasonable and not overstated?
Yes - the author's interpretation is reasonable
OVERALL MANUSCRIPT POTENTIAL - Is the current version of this work
technically sound? If not, can revisions be made to make the work technically
sound?
Yes - current version is technically sound
PEER REVIEWER COMMENTS: 
GENERAL COMMENTS: The overall impression about the manuscript is very
good. The authors present a report about the immunological variations in patients
with gastrointestinal cancer demonstrating significant alterations in pattern
presentation of T cells expressions in patients affected by cancer vs. unaffected
ones. 
They reported with details the technique used to demonstrate the hypothesis that
immunological response should be altered during cancer development. The work
does not have limits about best practices. It appears correctly conducted.
- the major findings of the study are that the immunological response varies in
cancer patients with gastrointestinal cancer. It is a fold of great interest in order
to better understand the mechanisms that are responsible of cancer development. 
- the method used is the classic statistical method after observation of an event.
The authors correctly performed the analysis comparing the results with that of
unaffected patients. Moreover, the authors give evidences about cytokines
detection in patients with GI cancer. 
- the work perfectly fits in the field of GI cancer research in order to understand
the evolution of cancer development. 
- these information should be the basis for all the authors for other evidences to
find.
Declaration of competing interests
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organization that holds or has applied for patents relating to the content of
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Do you have any other financial competing interests?
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Do you have any non-financial competing interests in relation to this
paper?
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interests' below. If your reply is yes to any, please give details below.
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recommendation.
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Is the study design appropriate to answer the research question (including the use
of appropriate controls), and are the conclusions supported by the evidence
presented?
Yes
Are the methods sufficiently described to allow the study to be repeated?
Yes
Is the use of statistics and treatment of uncertainties appropriate?
Yes
Is the presentation of the work clear?
Yes
Are the images in this manuscript (including electrophoretic gels and blots) free
from apparent manipulation?
Yes
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