Review for 10.1186/s12876-020-01467-w (2020)

Reviewer’ s report
Title: Carbon tetrachloride (CCl4) accelerated development of non-alcoholic fatty liver disease
(NAFLD)/steatohepatitis (NASH) in MS-NASH mice fed western diet supplemented with fructose(WDF)
Version: 0Date: 01 Aug 2020
Reviewer's report:
The manuscript entitled "Carbon tetrachloride (CCl4) accelerated development of non-alcoholic
fatty liver disease (NAFLD)/steatohepatitis (NASH) in MS-NASH mice fed western dietsupplemented with fructose (WDF)" is well written and very interesting because use novel modelof NASH which necessary foror testing novel therapeutics targeting NASH.Mnor revision:
In Introduction: instead Two-hit hypothesis... put a "multiple hit" hypothesis in which multiple
insults, such as insulin resistance, adipose tissue-derived factors, nutritional factors, gutmicrobiota, and genetic and epigenetic factors, work together and contribute to the pathogenesis ofNAFLD/NASH (Buzzetti et al., 2016)
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Is the study design appropriate to answer the research question (including the use of appropriate
controls), and are the conclusions supported by the evidence presented?
YesAre the methods sufficiently described to allow the study to be repeated?YesIs the use of statistics and treatment of uncertainties appropriate?YesIs the presentation of the work clear?
Yes
Are the images in this manuscript (including electrophoretic gels and blots) free from apparent
manipulation?
Yes