Review for 10.1186/s12876-020-01460-3 (2020)

Reviewer ’s report  
 
Title: Effects of Proton Pump Inhibitor Use on the Esophageal Microbial Community  
 
Version: 0 Date: 20 Jul 2020  
 
Reviewer's report:  
 
This study attempts to examine the effects of PPI use on the microbial community of the 
esophagus. The study concluded that PPI use does not seem to cause significant changes in the 
distal esophageal microbial Community  
 
I have certain comments  
 
- Would i t be possible to stratify the patients based on duration of PPI use, given the range is quite 
broad  
-Given the study included Barrett's patient only, can this have an affect on the results. Need to 
include this in limitation  
-Do you think sampling from dis tal esophagus only would have impacted the results?  
-Was there any impact of obesity and smoking status?  
 
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Yes 
 
Are the methods appropriate and well described?  
If not,  please specify what is required in your comments to the authors.  
 
Yes 
 
Does the work include the necessary controls?  
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Yes 
 
Are the conclusions drawn adequately supported by th e data shown?  
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Yes 
 
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statistical review?  
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I am able to assess the statistics  
 
Quality of written English  
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Acceptable  
 
Declaration of competing interests  
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'I declare that I have no competing interests'  
 
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