Review for 10.1186/s12876-021-01626-7 (2021)

Reviewer’s report
Title: 
Identification of Potential Biomarkers for Abdominal Pain in IBS Patients
by Bioinformatics Approach
Version: 
0 
Date: 
30 Sep 2020
Reviewer's report:
Dear Authors, 
I think this is an area that require more work and your research has given some
guidance in where and how to continue the quest.
Major comment
1) Please explain why IBS-D was chosen instead of IBS-C or IBS-M
Minor comment
1) All 3 figures are not clear and poor resolution. 
2) Need a better definition of VH
" visceral hypersensitivity (VH), which has been described as exaggerated
awareness of gastrointestinal balloon distension [2,3]
3) Please look for more up-to-date prevalence of IBS. 
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Is the study design appropriate to answer the research question (including the use
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