Review for 10.1186/s12876-020-01515-5 (2020)

Reviewer’s report
Title: 
HFD and HFD-provoked Hepatic Hypoxia act as Reciprocal Causation for
NAFLD via HIF-independent Signaling
Version: 
0 
Date: 
03 Jul 2020
Reviewer's report:
The manuscript HFD and HFD-provoked Hepatic Hypoxia act as Reciprocal
Causation for NAFLD via HIF-independent Signaling is well written, and very
interesting because it examines pathogenesis of NAFLD. 
This study investigate the interaction of lipotoxicity and hypoxia in the
pathogenesis of NAFLD using mice model with high-fat diet (HFD) feeding or
hypoxic treatment. NAFLD model was induced in mice by HFD feeding, and in
cultured primary
hepatocytes by administration of palmitate acid. HFD feeding and palmitate acid
treatment provoked severe hepatic hypoxia along with TG accumulation.
Hypoxia induced hepatic TG accumulation in mice and in cultured heptocytes.
Thus lipotoxicity and hypoxia might work as reciprocal causation and
orchestrate to promote the development of NAFLD.
The Results of this study are well done presented.
The manuscript is suitable for publication
Comment:
Change the title of the figures...for example The effect of HFD.....
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