Review for 10.1186/s12876-025-03853-8 (2025)

Title: Analysis of the distribution characteristics of pathogenic bacteria in the wound tissue of
patients with perianal abscess combined with infection and the influencing factors of wound
healing
Version: 2 Date: 19 Dec 2024
Reviewer:  Payam Behzadi
Reviewer's report:
Dear Author
Thank you for your manuscript submission; The current work is very interesting; however, the
presentation of the current work should be revised, rigorously:
1. The application of the terms should be unified: e.g., Gram-negative or gram-negative not
Gram-negative and gram-negative. There are several terms  that have been used the same
throughout the manuscript. Please do revise all of them.
2. In abstract section: The authors have claimed "The results of multifactorial logistic regression
analysis showed that body mass index, diabetes mellitus, concurrent infections and stool traits
were independent risk factors influencing the poor quality of wound healing (P < 0.05)." What
does P < 0.05 mean?
3. The name of microbial strains should be written in italic form. Please do revise all of them.
4. The keywords should be revised according to the MeSH terms. Please do revise them.
5. Although the topic of the manuscript is "Analysis of the distribution characteristics of
pathogenic bacteria in the wound tissue of patients with perianal abscess combined with infection
and the influencing factors of wound healing", the authors have mentioned no information
regarding the "GUT MICROBIOME". It is recommended to add some sentences in association
with the role of the "Gut Microbiome" in this regard. There are some effective papers that can
be useful as fresh References for the current manuscript:
Gut microbiota and immunity in health and disease: dysbiosis and eubiosis’s effects on the
human body. Front. Immunol. 15:1536258. doi: 10.3389/fimmu.2024.1536258
Systemic inflammation as the health-related communication tool between the human host and
gut microbiota in the framework of predictive, preventive, and personalized medicine. InAll
around suboptimal health: advanced approaches by predictive, preventive and personalised
medicine for healthy populations 2024 Feb 8 (pp. 203-241). Cham: Springer Nature Switzerland.
Molecular mechanisms and therapeutic possibilities of short-chain fatty acids in posttraumatic
stress disorder patients: a mini-review. Front Neurosci. 2024 Jun 3;18:1394953. doi: 10.3389/
fnins.2024.1394953. PMID: 38887367; PMCID: PMC11182003.
Current treatment options for leptospirosis: a mini-review. Front Microbiol. 2024 Apr
25;15:1403765. doi: 10.3389/fmicb.2024.1403765. PMID: 38725681; PMCID: PMC11081000.
6. The Methodology section suffers from the lack of hierarchical arrangement and references.
Please do revise this section and please do add the related references for the used protocols.
7.  Table 1 can be very effective if the current data presented by other items like gender and
the age range of the patients.
8. Figure 1 can be very effective if the current data presented by other items like gender and the
age range of the patients. Please do read the following paper which can be very useful reference
for the current manuscript:
Distribution of chaperone-usher fimbriae and curli fimbriae among uropathogenic Escherichia
coli. BMC Microbiol. 2024 Sep 14;24(1):344. doi: 10.1186/s12866-024-03472-5. PMID:
39271999; PMCID: PMC11401301.
9.  Please do interpret the results of the statistical analyses in Discussion section, in details.
10.  It is recommended to use the aforementioned references in Discussion section to have a
fruitful manuscript.
11. Please do revise the Discussion and Conclusion sections according to the aforementioned
comments.
12. Please do mention the strength and limitation of the current study.