Review for 10.1186/s12876-020-01453-2 (2020)

Reviewer’ s report
Title: ROLE OF SEX HORMONES AND THEIR RECEPTORS ON GASTRIC NRF2 AND
NEURONAL NITRIC OXIDE SYNTHASE FUNCTION IN AN EXPERIMENTAL HYPER-GLYCEMIA MODEL
Version: 1Date: 31 Jan 2020
Reviewer: Inge Bauer
Reviewer's report:
The manuscript is a follow-up study on the published paper (Sampath et al. 2019). The focus of
this manuscript is on gender and hyperglycemia. Although this is an in vitro study, the results addimportant information on the regulation and role of nNOS/Nrf2-signaling in diabetic gastroparesis.The study is well performed, the manuscript is well written.Comments:*Please provide the rationale of using such a high glucose concentration of 50 mM*The study was performed with only n=4 per group: please provide sample size calculation*The quality of the western blots (actin expression) could be improved: e.g. Figures 3A, 6D. Sincelines are not separated, it remains questionable how expression levels were assessed. Pleaseprovide blots in better quality and repeat analysis where necessary.*Page 8, line 53: 11 total groups, but on page 9: 12 groups are described*Did the authors assess the osmolarity of the culture media in different groups, e.g. NG, HG?*I look forward to the in vivo study in diabetic rodents
Are the methods appropriate and well described?
If not, please specify what is required in your comments to the authors.
YesDoes the work include the necessary controls?
If not, please specify which controls are required in your comments to the authors.
YesAre the conclusions drawn adequately supported by the data shown?
If not, please explain in your comments to the authors.
YesAre you able to assess any statistics in the manuscript or would you recommend an
additional statistical review?If an additional statistical review is recommended, please specify what aspects require further
assessment in your comments to the editors.
I recommend additional statistical review
Quality of written English
Please indicate the quality of language in the manuscript:
Needs some language corrections before being publishedDeclaration of competing interests
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