Review for 10.1186/s12876-020-01511-9 (2020)

Reviewer’s report
Title: 
Linked Color Imaging Improves Visibility of Reflux Esophagitis
Version: 
2 
Date: 
23 Jul 2020
Reviewer's report:
In the present paper, the authors presented "Linked Color Imaging Improves
Visibility of Reflux Esophagitis". The content of this paper is interesting.
However, I have the concerns about some weaknesses following below
comments.
Major comments:
1) The inclusion criteria are unclear in this study. What are the criteria of
enrolled patients? Selection bias could not have been avoided because there were
the possibilities that the authors selected the fine images of RE which was easily
diagnosed by LCI.
2) The samples are 142 lesions in this study. How did you calculate the sample
size of this study? It is afraid that setting of primary end point is insufficient. The
authors should describe it.
3) The authors did not describe how to select the ROI. Moreover, ROI was
selected only one area in RE and non-RE area. Therefore, selection bias could
not have been avoided. Please reconsider the methodology of color values.
4) I recommend interobserver variability for visibility score among expert or
trainee endoscopists.
Minor comments:
1) There was no information of color and structure enhancement setting in WLI
and LCI. Please describe them.
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interests' below. If your reply is yes to any, please give details below.
I received a research fund from Fujifilm Co., Ltd.
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Is the study design appropriate to answer the research question (including the use
of appropriate controls), and are the conclusions supported by the evidence
presented?
No
Are the methods sufficiently described to allow the study to be repeated?
Yes
Is the use of statistics and treatment of uncertainties appropriate?
Yes
Is the presentation of the work clear?
Yes
Are the images in this manuscript (including electrophoretic gels and blots) free
from apparent manipulation?
Yes