Review for 10.1186/s12876-017-0688-4 (2017)

Reviewer ’s report  
Title: Attenuating the Rate of Total Body Fat Accumulation and Alleviating  Liver Damage by  
Oral Administration of Vitamin D -enriched Edible Mushrooms in a Diet -induced Obesity 
Murine  Model is mediated by an Anti -inflammatory Paradigm Shift  
 
Version: 1 Date: 08 Jun 2017  
 
Reviewer: Kamilla Malinowska -Ozdowy  
 
Reviewer's report:  
The main message of the presented paper should be a beneficial effect of LE -mushrooms in 
NASH caused by the synergistic effects of Vitamin D and LE -extracts.  
The style of the manuscript has been improved however the content of the presented data stayed 
the same. The effects of LE mushrooms enriched in Vitamin D on all measured parameters were 
similar as for the treatments with LE mushrooms alone or Vitamin D alone. Therefore, I do not 
understand the benefit from using LE mushrooms enriched with Vitamin D. The only synergistic 
effect of LE mushrooms enriched in Vitamin D was seen on body lipid accumulation. The 
additional experiments providing the explanation for this effect were not presented however they 
were asked by reviewer. In my opinion the presented data do not support conclusions.  
 
Are the methods appropriate and well described?  
If not, please specify what is required in your comments to the authors.  
Yes 
Does the work include the necessary controls?  
If not, please specify which controls are required in your comments to the authors.  
Yes 
Are the conclusions drawn adequa tely supported by the data shown?  
If not, please explain in your comments to the authors.  
No 
Are you able to assess any statistics in the manuscript or would you recommend an  
additional statistical review?  
If an additional statistical review is recommende d, please specify what aspects require further  
assessment in your comments  to the editors.  
I am able to assess the statistics  
Quality of written English  
Please indicate the quality of language in the manuscript:  
Acceptable  
Declaration of competing interest s 
Please complete a declaration of competing interests, considering the following questions:  
1. Have you in the past five years received reimbursements, fees, funding, or salary from an  
organisation that may in any way gain or lose financially from the pub lication of this  
manuscript, either now or in the future?  
2. Do you hold any stocks or shares in an organisation that may in any way gain or lose  
financially from the publication of this manuscript, either now or in the future?  
3. Do you hold or are you cu rrently applying for any patents relating to the content of the  
manuscript?  
4. Have you received reimbursements, fees, funding, or salary from an organization that 
holds  or has applied for patents relating to the content of the manuscript?  
5. Do you have any other financial competing interests?  
6. Do you have any non -financial competing interests in relation to this paper?  
If you can answer no to all of the above, write 'I declare that I have no competing interests' 
below. If your reply is yes to any, please give details below.  
 
I declare that I have no competing interests.  
 
I agree to the open peer review policy of the journal. I understand that my name will be included 
on my report to the authors and, if the manuscript is accepted for publication, my named report 
including any attachments I upload will be posted on the website along with the au thors' 
responses. I agree for my report to be made available under an Open Access Creative Commons 
CC-BY license ( http://creativecommons.org/licenses/by/4.0/ ). I understand that any comments 
which I do not wish to be included in my named report can be incl uded as confidential comments 
to the editors, which will not be published.  
 
I agree to the open peer review policy of the journal