Review for 10.1186/s12876-021-01924-0 (2021)

Reviewer’s report
Title: 
Physiological and histopathological effects of electroporation pulse on
stomach of rats
Version: 
0 
Date: 
02 May 2021
Reviewer's report:
PEER REVIEWER ASSESSMENTS: 
OBJECTIVE - Full research articles: is there a clear objective that addresses a
testable research question(s) (brief or other article types: is there a clear
objective)?
Yes - there is a clear objective
DESIGN - Is the current approach (including controls and analysis protocols)
appropriate for the objective?
Yes - the approach is appropriate
EXECUTION - Are the experiments and analyses performed with technical rigor
to allow confidence in the results?
Yes - experiments and analyses were performed appropriately
STATISTICS - Is the use of statistics in the manuscript appropriate?
Yes - appropriate statistical analyses have been used in the study
INTERPRETATION - Is the current interpretation/discussion of the results
reasonable and not overstated?
Yes - the author's interpretation is reasonable
OVERALL MANUSCRIPT POTENTIAL - Is the current version of this work
technically sound? If not, can revisions be made to make the work technically
sound?
Yes - current version is technically sound
PEER REVIEWER COMMENTS: 
GENERAL COMMENTS: The study shows that EP is an attractive candidate
for gastric tissue ablation and has laid the foundation for wider use of this
technique in the future. 
corrections:
The animals were randomly divided into eight groups according to their time of
98 sacrifice: 0 h, 4 h, 1 d, 3 d, 7 d, 14 d, and 28 d post-EP and control groups. Is
control group and 0h groups are same. If not any treatment is given to control
group has to be described. 
Line 133: Site of blood collection to be included 
Line 161 and Table 1: The small intestine transit decreased from 67.71 to 33.25
and gradually recovered to 63.17 on day 7. On day 14 and day 28 again
decreased to 54.85 and 52.73 respectively. Why this recovery suddenly
decreased after day 7?
Line 167: Table 2 and Figure 2 appears same. Why repetition?
Overall impression of the study: satisfactory. The authors had addressed all the
ethical issues and carried out the study in a scientific way
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Is the study design appropriate to answer the research question (including the use
of appropriate controls), and are the conclusions supported by the evidence
presented?
Yes
Are the methods sufficiently described to allow the study to be repeated?
Yes
Is the use of statistics and treatment of uncertainties appropriate?
Yes
Is the presentation of the work clear?
Yes
Are the images in this manuscript (including electrophoretic gels and blots) free
from apparent manipulation?
Yes