Review for 10.1186/s12876-025-03597-5 (2025)

Title: The Association Between Oxidative Balance Score with Constipation and Diarrhea in US
Adults: A Cross-sectional Analysis of NHANES 2007–2010
Version: 2 Date: 26 Nov 2024
Reviewer:  Amira A A Othman
Reviewer's report:
First of all
I want to congratulate the authors on their work. In this study, Cao et al. used data from the 2007
to 2010 National Health and Nutrition Examination Survey (NHANES) for adult Americans.
This research investigates the association between Oxidative Balance Score(OBS) and chronic
constipation and diarrhea among US adult men and women. The study findings supported the
negative correlation between OBS and reduced likelihood of constipation.
Oxidative stress is closely related to gut health. Exposures to oxidative stress in one’s
diet and lifestyle can be evaluated by the oxidative balance score (OBS). However, the
relationship between OBS and intestinal habits is unknown. This study aimed to investigate the
relationships between OBS and intestinal habits (chronic diarrhea and chronic constipation) and
the underlying mechanisms involved*.
(*: /doi.org/10.1152/ajpcell.00283.2008  ).
The authors tried to contribute to this field of research with new findings, nevertheless, there is
an observation for which sufficient justification must be obtained.
In general:
1. Poor English writing, please seek the help of an English language specialist.
2. Abbreviations must be fully written at first appearance.
In Title:
3. The authors may consider indicating the locality at which the National Health and Nutrition
Examination Survey (NHANES) was performed and the age era of contributors.
I suggest “The Association Between Oxidative Balance Score with Chronic Constipation and
Diarrhea in US Adults: A Cross-sectional Analysis of NHANES 2007–2010” as an example.
4. The NHANES Survey encloses cycles from 2001 to 2020, I wondered about choosing the
2007-2010 cycle!
In Abstract section
5. The background subsection should be the shortest part of the abstract. It should briefly outline
the following information: What is already known about the subject, related to the paper in
question. What is not known about the subject and hence what the study intended to examine
(or what the paper seeks to present). Please reconstruct it properly.
6.  The type of study: a population-based study.
7. In method:
- please use “Using data from the National Health and Nutrition Examination Survey (NHANES)
database spanning from 2007 to 2010, a cross-sectional study included 4,462 participants was
performed to assess the relationship …….”.
- It would help if you described the aim of your statistical analysis.
- Nothing was mentioned about the participants’ sociodemographic characteristics.
8. The last two parts of an abstract are the results and the conclusions. You should include the
results that reflect the most important parts of your findings. You do not need to squeeze in every
detail of these results, and it's best to focus clearly on your most significant findings, without
any future directions.
In the Introduction section:
9. Try to provide a summarized description of The National Health and Nutrition Examination
Survey (NHANES) in the background section. You may use the following
“NHANES (www.cdc.gov/nchs/nhanes) is designed to assess the health and nutritional status
of adults and children in the United States. The survey is distinctive in that it incorporates
both physical examinations and interviews. Several cross-sectional, nationally representative
health examination surveys are part of the NHANES program. Questions about demographics,
health insurance status, dietary habits, acute and chronic medical issues, mental health, and
prescription drug use are all included in the health interview. Exam components can change
between survey cycles but typically include blood pressure, dental exams, vision, hearing,
dermatology, fitness, balance and strength testing, respiratory testing, taste and smell, and
body measurements (weight, height, skin folds, body composition scans). Hematology, organ
and endocrine function (e.g., thyroid, kidney), environmental exposure, dietary biomarkers,
metabolic and cardiovascular health, and infectious disease are a few examples of laboratory
components.”.
10. What about Constipation prevalence and constipation prevalence in Asia (China)?
11. “ The latest two study found that a higher OBS was negatively correlated with diarrhea and
positively correlated with constipation[21-22]……………”
-Last two studies (the manuscript is full of grammatical and constructive typos)
-This should be in the Discussion section through exploring the novelty of your study.
In the Methods section:
12. Please move the sentences defining the NHANES to the introduction section rather than the
methodology section.
13. How was constipation and/or diarrhea diagnosed? Is the information on constipation based
on the questionnaire “Ever told you had constipation?
14. The constipation information by questionnaire does not give any information on its type.
15. What was the source of the covariate data?
16. Statistical analysis:
- Data are expressed as mean ± standard error or mean ± standard deviation? Add to table footer
-   The Mann–Whitney U test is a nonparametric test and involves the summation of ranks. The
Mann–Whitney U test is applied to independent samples. T-test is used to compare the mean of
a continuous variable between a maximum of 2 groups. The Kruskal–Wallis test is a statistical
test used to compare two or more groups for a continuous or discrete variable. The Wilcoxon
rank-sum test is used to compare two independent samples, while the Wilcoxon signed-rank
test is used to compare two related samples, matched samples, or to conduct a paired difference
test of repeated measurements on a single sample to assess whether their population mean ranks
differ. How did you choose the proper test during your result analysis?
- In logistic regression analyses, is it multivariate or univariate? Please identify.
- “Three logistic regression models were employed to investigate the independent relationship
between Na intake and constipation, with varying levels of adjustment for potential confounders.
Model 1 was unadjusted for covariates, while Model 2 included adjustments for age, sex, and
race. Model 3 was further adjusted for education, marital status, PIR, BMI, alcohol intake,
smoking status, physical activity, hypertension, diabetes, high cholesterol levels, coronary heart
disease, stroke, and cancer.” Could you please explain how covariates were categorized?
- The generalized additive model and smoothed curve testing should be used to assess the
nonlinear relationship between circadian syndrome and gallstone disease.
In the Results section:
17. In Tables:
-You can title the statistical test column as “Test of significance” and use U= for the Mann–
Whitney U test, T= for the T-test, and #2 = for the Chi-square test, etc.
- abbreviations should be explained in the table foot.
18. Subgroup analyses must be performed to evaluate the effect of sarcopenia on gallstones and
their stability in different populations. Subgroup analyses based on differences in diagnostic
components were performed to elaborate correlations. The results were presented in forest plots
using odds ratios (ORs) and 95% confidence intervals (CIs).
19. Log2 logarithmic transformation shall be analyzed.
20. What about Quartile cut-off values?
In the Discussion section:
21. The discussion section is one of the final parts of a research paper, in which an author
describes, analyzes, and interprets their findings. They explain the significance of those results
and tie everything back to the research question(s). In this manuscript, there is confusion between
what should be written in the introduction section and the discussion section. Please reconstruct
the discussion section.
22. Using a questionnaire instead of a clinical diagnosis is to be considered a study limitation.
In the conclusion section:
23. The conclusion should restate your main points and provide closure.