Question: what are the essential features of a cloning vector?
Answer: Replication. Replication of plasmid DNA is carried by the same enzymes that replicate the E. ...  Selectable Markers (Antibiotic resistance) ...  Multiple cloning sites (or polylinkers) ...  Single-stranded DNA production. ...  Bacteriophage promoters. ...  Miniature Preparation of Bacterial Plasmid.