Question: what are the steps of pcr quizlet?
Answer: PCR. Polymerase chain reaction. Three steps. Denaturation. ...  Denaturation. 94 c for 30 sec separates DNA from being double stranded to single stranded. Primer annealing. 55 c for 45 sec oligonucleotide primers are able to bind on DNA strands. Primer extension. 72 c for 2 min uses thermophiles. ...  Paul berg.