JUNJIE99/VISTA_Evaluation huggingface dataset:
The use of the Suntag system allows multiple antibodies fused to VP64 to bind to dCas9-Suntag. That in turn recruits RNA polymerase and increases gene expression.
The dCas-SAM system uses msgRNA that has attached aptamers for different transcriptional factors (MS2,p65 and HSF1) to bind.
In situ-hybridization of Drosophila embryos at different developmental stages for the mRNA responsible for the expression of hunchback. High intensity of blue color marks places with high hunchback mRNA quantity.
The patchy colours of a tortoiseshell cat are the result of different levels of expression of pigmentation genes in different areas of the skin.
An edition of the original model of the Genesis, known as the Genesis III, was the model at the center of Sega v. Accolade for its incorporation of the Trademark Security System (TMSS).
Perimysium. Structure of a skeletal muscle. (Perimysium labeled at top center.)
Muscle biopsy. Micrographof a muscle biopsy showingragged red fibers, a finding seen in various types ofmitochondrial diseases.Gomori trichrome stain.
Bundle of His. Heart cut-away showing the bundle of His. Schematic representation of the atrioventricular bundle of His. The bundle, represented in red, originates near the orifice of thecoronary sinus, undergoes slight enlargement to form theAV node. The AV node tapers down into the bundle of His, which passes into theventricular septumand divides into two bundle branches, the left and right bundles. Sometimes the 'left and right bundles of His' are called Purkyně orPurkinje fibers. The ultimate distribution cannot be completely shown in this diagram.
Muscle spindle. Mammalian muscle spindle showing typical position in a muscle (left), neuronal connections in spinal cord (middle) and expanded schematic (right).  The spindle is a stretch receptor with its own motor supply consisting of several intrafusal muscle fibres. The sensory endings of a primary (group Ia) afferent and a secondary (group II) afferent coil around the non-contractile central portions of the intrafusal fibres. Gamma motor neurons activate the intrafusal muscle fibres, changing the resting firing rate and stretch-sensitivity of the afferents.[a]
Muscle fascicle. Structure of a skeletal muscle. (Fascicle labeled at bottom right.)
Rhomboid muscle. Muscles connecting the upper extremity to the vertebral column.
Swellings called varicosities belonging to an autonomic neuron innervate the smooth muscle cells.
Temporal muscle. The temporalis; thezygomatic archandmasseterhave been removed.
Clavicle (collarbone). Collarbone (shown in red)
The four main divisions of the spinal column, from top to bottom: cervical, thoracic, lumbar, and sacral
The picture displays the Mediastinum on sagittal plane, Thoracic diaphragm at the bottom, the heart (Cor), behind Sternum and Costae (to the left on the picture (This is the Anterior (front))) and to the right (Posterior (back)) you have the Thoracic vertebrae.
Thoracic ganglia. Lower half of right sympathetic cord.
Perpendicular plate of ethmoid bone. Ethmoid bonefrom behind.
Perpendicular plate of ethmoid bone. Perpendicular plate ofethmoid. Shown by removing the rightlabyrinth.
Superior nasal concha. Lateral wall of nasal cavity, showing ethmoid bone in position. (Superior nasal concha is at top of pink region.)
Inferior nasal concha. Medial wall of left orbit. (Inferior nasal concha visible in center in yellow.)
Inferior nasal concha. Figure 1: Lateral wall of right nasal cavity showing inferior conchain situ.(pink)
Maxillary process of inferior nasal concha. Rightinferior nasal concha. Lateral surface.
Ethmoidal process of inferior nasal concha. Right inferior nasal concha. Medial surface. (Ethmoidal process labeled at center right.)
Right and left inferior nasal conchae
Nasal concha/turbinate. Lateral wall of nasal cavity, showingethmoid bonein position. (Superior and middle in pink, and inferior in blue.)
Orbital lamina of ethmoid bone. The seven bones which articulate to form the orbit. (Ethmoid is brown.)
Lacrimal bone. Position of the lacrimal bone (shown in green).
Lacrimal bone. Medial wall of theorbit. Lacrimal bone is in yellow.
Lesser wing of sphenoid bone. Figure 2:Sphenoid bone, anterior and inferior surfaces.
Lacrimal sac. Thelacrimal apparatusisshown through dissection on the left side.
Lacrimal sac. The lacrimal sac has been opened showing internal organization as well as the naso-lacrymal duct.
Lacrimal gland. Lacrimal apparatusof the right eye. Thelacrimal glandis to the upper left. The right side of the picture is towards the nose.
Inferior rectus. Theinferior rectus muscle, is shown in this superior view of the eye, along with its axis of rotation.  The other muscle is the superior oblique muscle, which angles around the trochlea.
Polish POWs in Stalag II-B
Transcription preinitiation complex
Barrack hut of Stalag II B under construction, 1941
Memorial to 65,000 prisoners of war who died or were murdered by the Germans in Stalag II-B
The expanding remnant of SN 1987A, a peculiar Type II supernova in the Large Magellanic Cloud. NASA image.
This graph of the luminosity as a function of time shows the characteristic shapes of the light curves for a Type II-L and II-P supernova.[clarification needed]
Stalag II-B. A delegation of theInternational Red Crossled by Prof.Carl Jacob Burckhardtinspect POWs at Stalag II-B, 9 August 1941
Transcription factor II B. Crystallographic structureof transcription factor II B (top; rainbow colored,N-terminus= blue,C-terminus= red) complexed with double stranded DNA (bottom).
Fast carbon cycle showing the movement of carbon between land, atmosphere, and oceans in billions of tons per year. Yellow numbers are natural fluxes, red are human contributions, white are stored carbon. Effects of the slow carbon cycle, such as volcanic and tectonic activity, are not included.[255]
An aerial render of the spawn region in July 2019, centered on the middle of the map with a diameter of just over 4,000 blocks. The render displays the extreme amount of destruction and modification carried out to the terrain, including the construction of massive structures, such as the prominent Square and Compasses (upper right).
Brood parasites, such as the cuckoo, provide a supernormal stimulus to the parenting species.
Transcription of DNA to RNA using the protein RNA polymerase II.
Organic compounds such as glucose are vital to organisms
Secondary palate. A sagittal section of apig's (Sussp.) snout showing the secondary palate with pronouncedpalatine raphe.
Palatine tonsil. The Palatine tonsils with thesoft palate,uvula, andtonguevisible.
Palatine tonsil. Mouth (oral cavity)
Mylohyoid muscle. Muscles of theneckseen from the front (mylohyoid muscle colored in bright red)
SET7/9, a representative histone methyltransferase with SAM (red) and peptide undergoing methylation (orange. Rendered from PDB file 4J83.)
Epigenetic mechanisms
General scheme of the reaction catalyzed by a lysine histone methyltransferase
Third occipital nerve. Diagram of the distribution of the cutaneous branches of the posterior divisions of the spinal nerves. (Third cervical labeled at top.)
Cervical plexus. Dermatome distribution of the trigeminal nerve (Superficial cervical plexus visible in purple, at center bottom.)
The iHunch:  (1) Overworked muscles down the back of the neck strengthen, strain, scar and shorten; (2) middle and lower back support muscles weaken; (3) hunching upper back joints eventually freeze like that; (4) muscles at the front of the neck work less and weaken, so chin pokes out.  Results: Upper back pain, neck pain, headache; often referred pain down arms.
Lumbar plexus and branches
Ileal branch of ileocolic artery. Arteries ofcecumandvermiformprocess.
Ileocolic vein. Theportal veinand its tributaries.
Ileocolic artery. The superior mesenteric artery and its branches. (Ileocolic artery not labeled, but visible at bottom left.)
Ileal arteries. Loop ofileumshowing distribution of ileal arteries. The ileal veins follow a similar course.
Different characteristics of glands of the body
Diagrams that show the development of male and female organs from a common precursor
Stages in the development of the external sexual organs in the male and female: A: UndifferentiatedB: FemaleC: MaleD: FemaleE: MaleF: Female
Diagram showing how to measure the length and circumference of an erect human penis
Ancient Greek kouros sculpture from Anavyssos dating to c. 530 BC, showing an idealized young man with a small penisAncient Roman fresco from the House of the Vettii in Pompeii, dating to the first century AD, showing the god Priapus weighing his massive, permanently erect penis against a bag of gold[63][60]
Human penis. A flaccid penis, with surrounding pubic hair removed to show anatomical detail
Measuring erect micropenis
Vagina size
Penile enlargement procedures are designed to increase the size of the cavernous cylinders of the penis or to stimulate blood flow to increase hardness.
Anatomical diagram of a human penis
Corpus spongiosum penis. Lower Parts of the Genital and Urinary Tracts in the Male
Cross section of feet showing metatarsal bones forming anterior arch: A = normal position, B = flattened arch
Rossgraben bridge (Rüeggisberg) near Bern, Switzerland, showing the hinge at mid-span of this three-hinged arch.
Calcaneus fracture X-ray
3D still showing tarsal tunnel syndrome.
Landscape Arch in the Devils Garden, the longest in the park and the fifth-longest in the world
Tarsal Tunnel release
The plica semilunaris of conjunctiva is a vestigial remnant of a nictitating membrane in humans.
Plica semilunaris of the conjunctiva. Front of left eye with eyelids separated to show medial canthus. (Plica semilunaris labeled at center left.)
Camels at the Khan and old bridge, Lajjun, Palestine (now in Israel) - 1870s drawing
Carcharhinidae. CarcharhinidaeRequiem sharks1262Requiem sharks are migratory, live-bearing sharks of warm seas (sometimes of brackish or fresh water) such as thetiger shark, theblue shark, thebull shark, and themilk shark. The usual carcharhiniform characteristics include round eyes andpectoral finsthat are completely behind five gill slits. Most species areviviparous, the young being born fully developed. They vary widely in size, from as small as 69 cm (2.26 ft) adult length in theAustralian sharpnose shark, up to 5.5 m (18 ft) adult length in thetiger shark.[2]Requiem sharks are responsible for a large proportion ofattacks on humans.
Camels in the Guelta d'Archei, in northeastern Chad
When cats mate, the tomcat (male) bites the scruff of the female's neck as she assumes a position conducive to mating known as lordosis behavior.
Common goldeneye showing the transparent nictitating membrane characteristic of diving animals
Some cultures are superstitious about black cats, ascribing either good or bad luck to them
Frenulum breve.
Frenulum of labia minora. External genital organs of female. Thelabia minorahave been drawn apart (frenulum of labia minora is labeled asFrenulum pudendi)
Frenulum of prepuce.
Human neonatal dermal fibroblasts isolated from foreskin stained with calcein-AM. Such cells are commonly used in bioreactor and tissue engineering applications.
Shows the sub-areas of the clitoris. Areas include clitoral glans, body, crura. Also shows vestibular bulbs and corpora cavernosa.
Solitary nucleus. Transverse section ofmedulla oblongataof humanembryo.
Edinger–Westphal nucleus. Figure showing the different groups of cells, which constitute, according to Perlia, the nucleus of origin of the oculomotor nerve.1. Posterior dorsal nucleus.1’. Posterior ventral nucleus.2. Anterior dorsal nucleus.2’. Anterior ventral nucleus.3. Central nucleus.4.Nucleus of Edinger and Westphal.5. Antero-internal nucleus.6. Antero-external nucleus.8. Crossed fibers.9.Trochlear nerve, with 9’, its nucleus of origin, and 9", itsdecussation.10.Third ventricle.M, M. Median line.
Nucleus raphe magnus. Section of the medulla oblongata at about the middle of the olive. (Raphe nuclei not labeled, but 'raphe' labeled at left.)
Subcostal nerve. Cutaneous distribution ofthoracic nerves.
Thoracic spinal nerve. The spinal cord with spinal nerves.
Thoracic spinal nerve. The plan of the lumbosacral plexus
Referred pain: Conscious perception of visceral sensations is referred to specific regions of the body that are not sources of the sensations.  Some referred pain due to visceral sensations refer to dermatomes that send fibers to the same level of spinal cord.
A Crackerjack nutcracker, which uses a ratchet
Decorative brass populuxe nutcracker by the industrial designer Maurice Ascalon
Diagram of esophageal motility study in nutcracker esophagus: The disorder shows peristalsis with high-pressure esophageal contractions exceeding 180 mmHg and contractile waves with a long duration exceeding 6 sec.
A very large (9cm) fibroid of the uterus which is causing pelvic congestion syndrome as seen on ultrasound
Loin pain hematuria syndrome.
Ovarian vein syndrome.
SMA syndrome was first described in 1861 by Carl Freiherr von Rokitansky.
Global ice sheets may have created the bottleneck required for the evolution of multicellular life.[3]
Approximate extent of the Karoo Glaciation (in blue), over the Gondwana supercontinent during the Carboniferous and Permian periods
Thoracoacromial artery. Branches ofaxillary artery, including thoracoacromial artery
Transverse cervical artery. Superficial dissection of the right side of the neck, showing thecarotidandsubclavian arteries(transverse cervical artery is labeled, branching from thethyrocervical trunk)
Transverse cervical artery. Superficial and deep branches from the transverse cervical artery
Suprascapular artery. The scapular and circumflex arteries. (Transverse scapular visible at top.)
Pharyngeal branch of vagus nerve. Plan of upper portions ofglossopharyngeal,vagus, andaccessory nerves. (Pharyngeal visible at center right.)
Recurrent laryngeal nerve visible during resection of a goitre
Schematic image of the hypoglossal nerve and innervation targets.
Pharyngitis is the painful swelling of the throat. The oropharynx shown here is very inflamed and red.
Hypoglossal nerve. Hypoglossal nerve,cervical plexus, and their branches.
Upper respiratory system, showing entrance to auditory tube near middle.
Eustachian tube. Middle ear, with auditory tube at bottom right
Eustachian tube. External and middle ear. Eustachian tube labelled as auditory tube
An endotracheal tube not deep enough. Arrow marks the tip.
A cuffed endotracheal tube, constructed of polyvinyl chloride
An endotracheal tube in good position on CXR. Arrow marks the tip.
3 D Reconstruction of the right Eustachian tube from Valsalva CT. Note the sac like shape of the structure.
3D reconstruction of the air cell system of the temporal bone including the eustachian tube and its narrowest area in close proximity to the ear, the isthmus.
A Carlens double-lumen endotracheal tube, used for thoracic surgical operations such as VATS lobectomy
Diagram of a tracheostomy tube in the trachea: 1 - Vocal folds 2 - Thyroid cartilage 3 - Cricoid cartilage 4 - Tracheal rings 5 - Balloon cuff
Gonadal vein. The relations of the viscera and large vessels of the abdomen .(as seen from behind)
Suprarenal veins. The anterior surfaces of the kidneys, showing the areas of contact of neighboring viscera.
Common iliac vein. Abdominal portion of thesympathetic trunk, with the celiac and hypogastric plexuses.  (Common iliac vein labeled at lower right.)
Upper extremity DVTs can occur in the subclavian, axillary, brachial, ulnar, and radial veins (pictured) and the jugular and brachiocephalic veins (not pictured). The cephalic and basilic veins, however, are superficial veins.[11]
Testicular vein. Spermatic veins.
left atrial appendage shown at upper right
The human heart is in the middle of the thorax, with its apex pointing to the left.[14]
Basic hemoglobin saturation curve. It is moved to the right in higher acidity (more dissolved carbon dioxide) and to the left in lower acidity (less dissolved carbon dioxide)
The iliac veins (in the pelvis) include the external iliac vein, the internal iliac vein, and the common iliac vein. The common femoral vein is below the external iliac vein. (It is labeled simply "femoral" here.)
Right heart anatomy
Enlarged view from the front of the left Wolffian body before the establishment of the distinction of sex.a, b, c, d. Tubular structure of the Wolffian body.e. mesonephric duct.f. Its upper extremity.g. Its termination in x, the urogenital sinus.h. The duct of Müller.i. Its upper, funnel-shaped extremity.k. Its lower end, terminating in the urogenital sinus.l. The genital gland.
Mesonephric duct. Urogenital sinusof female humanembryoof eight and a half to nine weeks old.
Image showing the bottom part of an embryo 4 - 5 weeks old. Here, the ureter (in orange) can be seen emerging from the bottom of the mesonephric duct (labelled "Wolffian duct"), connected to the primitive bladder. Image from Gray's Anatomy 1918 edition.
Urogenital sinus. Stages in the development of the external sexual organs in the male and female. ("Opening of urogenital sinus" labeled in diagram D.)
Paramesonephric duct. Tail end of human embryo, from eight and a half to nine weeks old.
Gartner's duct cyst.
Wheat plantation in Paraná
Temperature change in Paraná since 1901 in the context of global warming
Trinational Biodiversity Corridor.
Trinational Biodiversity Corridor. TheIguazu Fallsare in the center of the proposed corridor
Amapá Biodiversity Corridor. Waterfalls in theJari River
Amapá National Forest
Temperature change in Amapá since 1901 in the context of global warming
Amapá. Karupina mountain in the Uaçá Indigenous Territory
Amapá mangroves (NT1402). Avicennia schauerianain theCabo Orange National Park
Dynamic of DNA methylation during mouse embryonic development. E3.5-E6, etc., refer to days after fertilization. PGC: primordial germ cells
Figure 3. A representation of the PBAD 33 promoter on a plasmid with common cis-acting regions.  Abbreviations are defined as the phagemid origin (f1 origin), chloramphenicol resistance (CmR), plasmid origin (p15A ori), araC gene (araC), araC operator sites (araC O2 and O1), CAP-binding site (CAP BS), araC inducer sites (I1/I2), PBAD promoter (pBAD) and the multiple cloning site (MCS).
Electron micrograph of a bacterial DNA plasmid (chromosome fragment)
A diagram showing the process of inserting a multiple cloning site into a plasmid vector.
A pUC19 cloning vector showing the multiple cloning site sequence with restriction enzyme sites.
Figure 2. Expression of araB, araA and araC does not occur when arabinose is not present. In the absence of arabinose, AraC dimerizes while bound to the O2 and I1 operator sites, looping the DNA. The looping prevents binding of CAP and RNA Polymerase, which normally activate the transcription of both PBAD and PC.
The MODAL standard provides a common format to allow any DNA part to be made compatible with Gibson assembly or other overlap assembly methods. The DNA fragment of interest undergoes two rounds of PCR, first to attach the adaptor prefix and suffixes, and next to attach the predefined linker sequences. Once the parts are in the required format, assembly methods like Gibson assembly can carried out. The order of the parts is directed by the linkers, i.e the same linker sequence is attached to the 3' end of the upstream part and the 5' end of the downstream part.
DNA parts to be used for BASIC assembly need to contain integrated prefix and suffix sequences (iP and iS). These contain BsaI restriction sites that will allow for the iP and iS linkers (which contain complementary overhang sequences) to be attached to the DNA part. Once the linkers are attached, the part is ready for assembly.The sequence of assembly is directed by the positioning of the linkers. The suffix linker is ligated to the 3' end of the upstream fragment, while the prefix linker is ligated to the 5' end of the downstream fragment. Shown here is an example of a BASIC assembly construct using four DNA parts.
Type II site-specific deoxyribonuclease-like. Structure of thehomodimericrestriction enzymeEcoRI(cyan and green cartoon diagram) bound to double strandedDNA(brown tubes).[38]Two catalyticmagnesiumions (one from eachmonomer) are shown as magenta spheres and are adjacent to the cleaved sites in the DNA made by the enzyme (depicted as gaps in the DNA backbone).
The sequence of DNA parts for the Golden Gate assembly can be directed by defining unique complementary overhangs for each part. Thus, to assemble gene 1 in order of fragment A, B and C, the 3' overhang for fragment A is complementary to the 5' overhang for fragment B, and similarly for fragment B and fragment C. For the destination plasmid, the selectable marker is flanked by outward-cutting BsaI restriction sites. This excises the selectable marker, allowing the insertion of the final construct. T4 ligase is used to ligate the fragments together and to the destination plasmid.
Restriction Fragment Length Polymorphism
Shared DNA for different relatives
DNA Double Helix
Typical DNA methylation landscape in mammals
Alec Jeffreys, a pioneer of DNA profiling.
CBP chemist reads a DNA profile to determine the origin of a commodity.
Figure 1. Expression of araB, araA and araC in the presence of arabinose. In the presence of arabinose, arabinose binds to the arabinose binding pocket sites of AraC, causing AraC to dimerize at the I1 and I2 operators. This allows access for CAP to bind to the CAP-binding sites, which in turn helps recruit RNA Polymerase to both PBAD and PC promoters and activates transcription.
Restriction enzyme Eco RI
Funduscopic photo left eye centered on the optic disc
Optic foramen. 1:Foramen ethmoidale2:Canalis opticus3:Fissura orbitalis superior4:Fossa sacci lacrimalis5:Sulcus infraorbitalis6:Fissura orbitalis inferior7:Foramen infraorbitale
Optic radiation. Colour-coded diagram showing radiations in quadrants from retinal disc through the brain
Optic nerve glioma.
Optic disc cross-sections imaged by an SD-OCT.
A juvenile spotted tilapia approx 5 cm (2 in)
Spotted tilapia.
Spotted tilapia caught at the heated outflow of a powerplant in southern Australia
Redbelly tilapia.
Red nile tilapia under experimentation in CLSU, Philippines
Escabeche fresh tilapia
Nile tilapia (Oreochromis niloticus)
Blackened tilapia filets seasoned with Cajun spices, lemon & lime juice
The Tomb of Nakht, from 1500 BC, contains a tilapia hieroglyph just above the head of the central figure.
Global harvest of tilapia species in million tonnesas reported by the FAO, 1950–2009 [35]↑ Wild capture↑ Aquaculture production
Redbelly tilapia, Tilapia zillii ("St. Peter's fish") from the Sea of Galilee served in a Tiberias restaurant
Nile tilapia, Oreochromis niloticusGlobal harvest of tilapia in million tonnes as reported by the FAO, 1950–2009[1]
TilapiaAquaculture production of tilapia by speciesin million tonnes as reported by the FAO, 1950–2009[1]Aquaculture production of tilapia by countryin million tonnes as reported by the FAO, 1950–2009[1]
Secretion and reabsorption of various substances throughout the nephron
Fig.1) Schematic diagram of the nephron (yellow), relevant circulation (red/blue), and the four methods of altering the filtrate.
Descending limb of loop of Henle. Nephron ion flow diagram
Coronal suture. Side view of the skull. ("Coronal suture" in red.)
Coronal suture. Superior view of the skull. ("Coronal suture" in red.)
Cartoon illustrating the difference between bilateral (Drosophila), radial (actinomorphic flowers) and spherical (coccus bacteria) symmetry
The anatomical axes of orientation of the human brain are at odds with the anatomical axes of the human body in the standard anatomical position.Red axis shows how the head bent forward as the back pointed upwards: c: Caudal r: Rostral Yellow axes show the conventions for naming directions in the brain itself: c: Caudal (though not tail direction), d: Dorsal r: Rostral (effectively unchanged) v: Ventral (though not belly direction)
A skull is the symbol of penance, Silk embroidery (17th century)
Lambdoid suture. Lambdoid suture (shown in red line)
Lambdoid suture. Lambdoid suture (labeled at bottom right)
Wormian bones(Sutural bones). Skull of a 21-year-old man with Wormian bones