Should I use cell lines or primary cells for my experiment?
STEMCELL recommends that you use primary cells for your experiments where possible. Human primary cells are increasingly recognized as an ideal model for your experiment—data obtained from in vitro assays or in vivo models using these cells have increased physiological relevance compared to those performed on cell lines. Primary cells are isolated directly from tissues, including blood and bone marrow, and are therefore more representative of the tissue being investigated than cell lines.