Are you using the same fiber type as in the adjustment procedure?
The fibers used in the standard adjustment procedure are all equipped with an end cap when aligning for wavelengths ≤ 520 nm. The adjustment wavelength is given on the label for each collimator/coupler. If a fiber with end cap was used it is marked by "EC".
If you are not using a fiber with an end cap but the preadjustment at Schäfter+Kirchhoff was done using an end cap ("EC") or you are using a fiber with an end cap and the preadjustment at Schäfter+Kirchhoff was done without, you might need to change the focus setting. Please refer to the manual for more details.