What is the purpose of insertional inactivation as a selectable marker to differentiate recombinant from non-0recombinant proteins?
Alpha-galactosidase is inactivated by insertional inactivation, which is caused by the insertion of rDNA into its coding sequence. In the presence of a chromogenic substrate, the recombinant bacteria do not produce a blue color while the non-recombinant bacteria produce a blue color.