Pos From To Mol Wt @ Cho Aro Acid Base Sul Phil Phob 7 176 - 176 133.1 -1.0 0 1 0 0 1 0 8 177 - 178 296.3 -1.0 1 1 0 0 1 1 3 135 - 138 461.5 -1.0 0 2 1 o 3 1 6 170 - 175 658.7 -2.0 0 2 0 1 4 2 4 139 - 146 1146.2 -2.0 3 2 O 1 4 4 12 220 - 229 1193.4 0.0 1 2 2 0 5 5 9 179 - 188 1225.4 -2.0 2 2 0 1 4 6 13 230 - 244 1663.8 -1.0 3 3 2 0 10 4 11 205 - 219 1697.0 0.0 0 3 3 o 9 6 10 189 - 204 1907.1 -3.0 1 4 1 C 10 6 5 147 - 169 2697.9 -5.0 3 S 0 1 12 11 1 1 - 50 5230.0 0.0 4 3 3 1 22 28 2 51 - 134 9317.5 3.0 7 7 42 42 Annexe 3 Pos AA GlycoS HyPhil AI-Ind 1 M 0.100 0.450 2 P . 0.260 0.450 3 N . -0.083 0.250 4 F . -0.614 -0.050 5 W . -0.114 0.100 6 A . -0.114 -0.300 7 L . -0.114 -0.300 8 P . -0.357 -0.150 9 P 0.014 0.650 10 E 0.386 1.200 il I . 1.029 1.300 12 N G 1.443 1.300 13 S . 0.571 1.150 14 T . 0.257 0.600 15 R . 0.357 0.300 16 I . -o.oa6 -0.300 17 Y . 0.029 0.300 18 L . -0.014 -0.300 19 G . -0.543 -0.450 20 P . 0.157 1.250 21 C . 0.200 1.250 22 S . 0.100 0.850 23 C . -0.500 -0.050 24 P . -0.986 -0.450 25 I . -1.300 -0.600 26 L . -1.671 -0.600 27 A . -1.229 -0.600 28 A . -1.400 -0.600 29 A . -0.629 -0.600 30 Q . 0.414 0.300 31 G . 0.414 0.300 32 W . 0.129 0.300 33 N . 0.129 0.300 34 A . -0.257 -0.300 35 L . 0.186 0.300 36 A -0.486 -0.600 37 S . -0.486 -0.600 38 E . 0.329 0.600 39 L . 0.971 0.900 40 E 1.786 0.900 41 K . 1.071 0.900 42 T . 0.629 0.900 43 K 0.629 0.750 44 V 0.629 0.750 45 G 0.186 0.300 46 L -0.171 -0.300 47 Q -1.271 -0.600 48 S -0.171 -0.300 49 A -0.129 0.000 50 L -0.129 -0.150 51 D -1.171 -0.450 Annexe 3(suite 1) 52 T -0.786 -0.450 53 L -0.414 -0.450 54 @ 0.314 0.450 55 E 0.457 1.000 56 S 0.414 1.200 57 Y 1.457 1.500 58 R 2.114 1.700 59 G 1.729 1.700 60 Q 2.114 1.500 61 S . 1.671 1.100 62 S . 0.486 0.800 63 Q . -0.214 -0.150 64 A -0.214 -0.300 65 L 0.171 0.300 66 I . 0.157 0.300 67 Q . -0.886 -0.450 68 q . -0.400 -0.450 69 T . 0.329 0.600 70 L 0.37l 0.300 71 P 0.371 0.300 72 Y . 0.000 0.300 73 V -0.643 -0.600 74 Q 0.000 0.300 75 W . -0.129 -0.300 76 L . -0.214 -0.150 77 T . 0.129 0.450 78 T 0.129 0.450 79 T 0.457 0.600 80 A 0.743 0.900 81 E . 1.100 0.750 82 H 1.557 0.750 83 A . 1.557 0.750 84 H . 1.557 0.750 85 X . 0.414 0.300 86 T . 0.457 0.450 87 A . 0.171 0.300 88 I . -0.186 -0.300 89 Q . -1.000 -0.600 90 L . -1.357 -0.600 91 T . -1.357 -0.600 92 A . -0.214 -0.300 93 A . -0.971 -0.600 94 A . -0.243 -0.300 95 N . 0.157 0.450 96 A 0.914 0.750 97 Y . 0.914 0.750 98 E . 1.814 0.750 99 Q @ 1.057 0.750 100 A . 1.057 0.750 101 R . 0.600 0.600 102 A @ -0.500 -0.600 103 A -0.771 -0.600 104 M . -0.286 -0.300 105 V -1.186 -0.600 106 P -1.200 -0.600 107 P -1.543 -0.600 108 A -0.629 -0.600 109 M -0.286 -0.300 110 V -0.014 -0.300 111 R 0.400 0.450 112 A 0.057 0.300 113 N 0.829 0.750 114 R 1.529 0.900 115 V 0.985 0.900 116 Q 0.643 0.750 117 T -0.400 -0.450 Annexe 3 (suite 2) 118 T -0.486 -0.300 119 V -0.143 -0.300 120 L -1.286 -0.600 121 X -0.886 -0.600 122 A -0.857 -0.600 123 I -0.657 -0.600 124 N -0.057 0.100 125 w . -0.114 0.100 126 F . -0.257 -0.300 127 G 0.500 0.700 128 Q 0.100 0.700 129 F 0.614 0.900 130 S 0.371 0.450 131 T 0.057 0.450 132 R 0.057 0.600 133 I 1.014 0.750 134 A 1.400 0.900 135 D 1.043 0.750 136 K . 0.900 0.900 137 E 1.729 0.900 138 A . 2.486 0.900 139 D 2.486 0.750 140 Y 1.657 0.750 141 E 1.286 0.750 142 Q 1.143 0.600 143 M . 1.143 0.750 144 W . 1.457 0.750 145 F . 0.700 0.600 146 Q . -0,343 -0.300 147 D . -0.671 -0.600 148 A . -1.071 -0.600 149 L . -0.171 -0.300 150 V . -0.171 -0.300 151 M . -0.486 -0.600 152 E @ -0.100 -0.300 153 N . 0.943 0.950 154 Y . 1.286 0.950 155 W . 0.957 0.600 156 E . 0.957 0.600 157 A . 0.957 0.600 158 V . 0.514 0.600 159 Q . -0.257 -0.300 160 E . -0.257 -0.300 161 A . 0.114 0.450 162 I . 0.814 0.750 163 Q . 0.429 0.450 164 S . 0.386 0.450 165 T . 0.243 0.800 166 S . 1.386 1.100 167 H . 1.386 0.900 168 F . 1.500 0.750 l69 E 1.629 0.900 170 D 2.014 0.900 171 P 1.286 1.100 172 P 1.429 1.100 173 E 1.429 0.900 174 M . 1.429 0.750 175 A . 1.386 0.750 176 D 1.657 0.900 177 D 1.657 0.900 178 Y 1.671 0.900 179 D 2.057 0.900 180 E 1.286 0.750 181 A 0.243 0.330 182 W 0.557 0.600 183 M 0.157 0.300 Annexe 3 (suite 3) 184 L -0.943 -0.600 185 N -1.086 -0.600 186 T -0.714 -0.600 187 V -0.257 -0.300 188 F 0.743 0.600 189 D 0.743 0.600 190 Y 1.143 0.950 191 H 2.243 0.950 192 N 2.386 0.900 193 . E 2.443 0.900 194 N . 2.757 0.900 195 A . 2.800 0.900 196 K 1.700 0.900 197 E 0.557 0.750 198 E . 0.514 0.750 199 V . 0.229 0.300 200 I . -0.929 -0.600 201 H . -1.200 -0.600 202 L -1.200 -0.600 203 V -1.200 -0.600 204 P . -0.057 -0.150 205 D 0.043 0.600 206 V @ 1.086 0.900 207 N @ 2.329 0.900 208 K @ 2.157 0.900 209 E @ 1.886 0.900 210 R 1.843 0.900 211 G 1.843 0.900 212 P @ 0.743 0.750 213 I @ -0.357 -0.150 214 E -0.900 -0.600 215 L . -0.400 -0.300 216 V . -1.229 -0.600 217 T . -0.086 -0.150 218 K . -0.029 0.000 219 V @ 1.014 0.900 220 D @ 1.671 0.900 221 K @ 1.671 0.900 222 E @ 0.471 0.600 223 G @ 1.714 0.900 224 T 0.671 0.750 225 I -0.486 -0.450 226 R -0.800 -0.600 227 L . -0.357 -0.300 228 V . -0.400 -0.300 229 Y @ 0.743 0.600 230 D @ 0.329 0.800 231 G @ 0.971 1.100 232 E 1.171 0.900 233 P 1.100 0.900 234 T 0.786 1.100 235 F . 1.286 0.950 236 S 1.286 0.750 237 Y 1.514 0.750 238 K 1.643 0.900 239 E 2.600 0.500 240 H 2.086 0.900 241 P 2.217 1.700 242 K 1.880 1.700 243 F 1.475 1 300 LISTE DE SEQUENCES (1) INFORMATIONS GENERALES: (1) DEPOSANT: (A) NOM: INSTITUT PASTEUR (B) RUE: 28 RUE DU DOCTEUR ROUX (C) VILLE: PARIS CEDEX 15 (E) PAYS: FRANCE (F) CODE POSTAL: 75724 (ii) TITRE DE L' INVENTION: POLYNUCLEOTIDE CODANT POUR UN POLYPEPTIDE DE 27KD DE MYCOBACTERIES APPARTENANT AU COMPLEXE DE MYCOBACTERIUM TUBERCULOSIS. APPLICATION AU DIAGNOSTIC ET A LA PREVENTION DE LA TUBERCULOSE (iii) NOMBRE DE SEQUENCES: 3 (iv) FORME DECHIFFRABLE PAR ORDINATEUR: (A) TYPE DE SUPPORT: Floppy disk (B) ORDINATEUR: IBM PC compatible (C! SYSTEME D' EXPLOITATION: PC-DOS/MS-DOS )D) LOGICIEL: PatentIn Release 1.0, Version #1.30 (OEB) 2) INFORMATIONS POUR LA SEQ ID NO: 1: (1) CARACTERISTIQUES DE LA SEQUENCE: (A) LONGUEUR: 2805 aires ce jase B) TYPE: nucl otide NOMBRE DE BRINS: simple (D) CONFIGURATION: lineaire (ii) TYPE DE MOLECULE: ADN (g nomique) (xi) DESCRIPTION DE LA SEQUENCE: SEQ ID NO: 1: TTGGGCCGCC GTAAGGAATG CGTCATGAGC GACTTCGCAT CACGGGCGAC CAATCATTAA 60 TTTGTCAAAC CCTTTGAGAT GCACTACTTG TCCACATTTT GTACACGAAA TACCTAACAC 120 ACTATGGTGC ACATCACGCA CTTCCACGTT CCGTATTCGG TGTACGATTT GTCACGCAAC 180 TAAGCGTTCA AGAGGGAGTA CTATGACTCA CCAAAAGTAA AAGATGACAT AGAAATAGAA 240 GAGTCGTGGT TCCGGTGCGG GTAGCTCCCG ATGGCTTGAC TGTGGTAAGC ACCAGTGGCG 300 TGTTCCCCGT GGTTGAGACC AGGAAGTTTT AAAGTCCTAC AGCCCGCGGT ATTCCGCAGA 360 GGACATTGTG TGCATTTCGC ACCTTCGGGT GGGAGAAATC GGGATGATCT CACCACCGGC 420 CACCGGGTGG CGCACTTTGT ACCCTTCGAT TCCGTTATTC GGCGGATTTA AGCAGTTCGC 480 ACCATTACCA AGCAGCCAAT GAGGAAGAGC GCAGGTGACT AGGTCGCTTG ATCTTTCCCT 540 CTGCAGTAGC TCGGGTTCTT TGAGTTTCGA GGAGGAGAAA CCACATGTCC TTTGTGAATG 600 TA--ACCCATT TGGATGTTGG CGGCCGTGCG ACACTGGAGT CCCTTCGTTC CCACATGGCG 660 @TAAGCAATG CCGCGGTGGC CTCGGTGACC ACCAAGGTTC CTCCCCCGGC CGCCGACTAC @@@TCAAAAA AGTTATCGCT GTTCTTTAGT AGCCACGGGC AGCAGTACCA @@TGCAAGCC @@TCGGGCAC GGCCTTTCAT CGAAATTGGT CCGGACCCTG GGCCGAATTG CGCTTGCGTA TGAGGAAGTC GAGAATCGCC AACAACGAAG GTTTCTAACG TGTCGCCAGT TACGCACGAG 900 TGGCTACCAG CGAGTACAAG GGAGTAACGA ATTATGCCCA ATTTCTGGGC GTTGCCGCCC 960 GAGATCAACT CCACCCGGAT ATATCTCGGC CCGGGTTCTG GCCCGATACT GGCCGCCGCC 1020 CAGOGATOGA ACGCTCTGGC CAGTGAGCTG GAAAAGACGA AGGTGGGGTT GCAGTCAGCG 1080 CTCGACACGT TGCTGGAGTC GTATAGGGGT CAGTCGTCGC AGGCTTTGAT ACAGCAGACC 1140 TTGCCGTATG TGCAGTGGCT GACCACGACC GCCGAGCACG CCCATAAGAC CGCGATCCAG 1200 CTCACGGCAG CGGCGAACGC CTACGAGCAG GCTAGAGCGG CGATGGTGCC GCCGGCGATG 1260 GTGCGCGCGA ACCGCGTGCA GACCACAGTG TTGAAGGCAA TCAACTGGTT CGGGCAATTC 1320 TCCACCAGGA TCGCCGACAA GGAGGCCGAC TACGAACAGA TGTGGTTCCA AGACGCGCTA 1380 GTGATGGAGA ACTATTGGGA AGCCGTGCAA GAGGCGATAC AGTCGACGTC GCATTTTGAG 1440 GATCCACCGG AGATGGCCGA CGACTACGAC GAGGCCTGGA TGCTCAACAC CGTGTTCGAC 1500 TATCACAACG AGAACGCAAA AGAGGAGGTC ATCCATCTCG TGCCCGACGT GAACAAGGAG 1560 AGGGGGCCCA TCGAACTCGT AACCAAGGTA GACAAAGAGG GGACCATCAG ACTCGTCTAC 1620 GATGGGGAGC CCACGTTTTC ATACAAGGAA CATCCTAAGT TTTGATTCGG GAACATCCTA 1680 @@AAACGGGG GGCGTCGCCG TTGGAGACGT CGCAACGTGT CCGCAGTCCC AAGGGCAACA 1740 @@@AAGGGCC CACGGTGCGA TCCCCAACAC CCGGCTAGAG TGCGCATAAT ATTTTCCCGC 1800 @@@@GCTCAA GGCGTGCACC CCCATCACCG CTAACCATGC TGTGTATCAA CAGATTTCAT 1860 TGTCCCGGCC GTCGCGCGAC CGACCAATAG GGTGAGTTCC ATGTGCGATA TCGCCTAACA 1920 GCCGGCTC@C GTACTCCCGT GGCCGATGTG ATTATTGATT ACGTGGATCA CCATGTGGGT 1980 GATCGCGGTC GACAGCTTTG GTACCGAGCA CATCGCCACA ACGCGCGGTA CGAATCTAGT 2040 ACACAAATCC GCACCAGCCG CCATGCGACT TCGCAGGTCA TAGCCCCGCA GAGTCGCCGA 2100 ACCTGCCGCA GTGACAAAAG TCAGGACGGC CGGCGACGCG TCGAGCCGGG GTTAGGCGCA 2160 GTTAACGTCG CAGCGGGGTC CCAGACACGC GTCGGACTTT CGGACTCAGC CCGACGATTC 2220 GCCGTCAGAC TGCGGGCTTT CCTGGTCTAC CAGCAACGCT TGCAGGGCGG AGCCGGTGAT 2280 GCGCCGGAAC GCGCGCCGTG GCCGGTTGGC ATCGAGAACG GCCACCTCTA AGCTGGCCAC 2340 GCCAAGGGTG GGTTGATCAC CACCCGAGGT GTCGGCACTG CCGGCCCGCA ATGCAGCGAC 2400 CGCGATACGC AGGGCGTCGG TCAGGCTGGC GTTCTCGGCA TACGACTCTT TGAGCGCGTT 2460 GGCGATCGGC TCCGTGGTGC CGCCCATCAC CACGAAATGC GGCTCGTCGG CGATCGACCC 2520 GTCGTAGGTA ATACGATACA ACTCAGGGCG TTTCGTCTCG CCGTAATGCG CCACCTCGGC 2580 CACACACAAC TCAACCTCGT AGGGCTTGGC CTGTTCGGTG AAGATGGTGC CTAGAGTCTG 2640 CGCGTAGACA TTGGCCAACT GCCGACCCGT GACGTCACGA CGGTCATAGG CGTAACCGCG 2700 CGTGTCGGCG AACTGGATCC CGCCGCGGCG CAAATTGTCG AACTCGTTGA ACTTGCCCGC 2760 @@@@GCAAAA CCCACCCGAT CGTAGAGCTC ACTGATCTTC TG@@ 2805 INFORMATIONS POUR LA SEQ ID NO: @ CARACTERISTIQUES DE LA SEOJENCE: (A LONGUEUR: @32 paires de bases (B) TYPE: nucl otide (C) NOMBRE DE BRINS: simple (D) CONFIGURATION: lin aire (ii) TYPE DE MOLECULE: ADN (g nomique) (xi) DESCRIPTION DE LA SEQUENCE: SEQ ID NO: 2: ATGCCCAATT TCTGGGCGTT GCCGCCCGAG ATCAACTCCA CCCGGATATA TCTCGGCCCG 60 GGTTCTGGCC CGATACTGGC CGCCGCCCAG GGATGGAACG CTCTGGCCAG TGAGCTGGAA 120 AAGACGAAGG TGGGGTTGCA GTCAGCGCTC GACACGTTGC TGGAGTCGTA TAGGGGTCAG 180 TCGTCGCAGG CTTTGATACA GCAGACCTTG CCGTATGTGC AGTGGCTGAC CACGACCGCC 240 GAGCACGCCC ATAAGACCGC GATCCAGCTC ACGGCAGCGG CGAACGCCTA CGAGCAGGCT 300 ACAGCGGCGA TGGTGCCGCC GGCGATGGTG CGCGCGAACC GCGTGCAGAC CACAGTGTTG 360 AAGGCAATCA ACTGGTTCGG GCAATTCTCC ACCAGGATCG CCGACAAGGA GGCCGACTAC 420 GAACAGATGT GGTTCCAAGA CGCGCTAGTG ATGGAGAACT ATTGGGAAGC CGTGCAAGAG 480 GCGATACAGT CGACGTCGCA TTTTGAGGAT CCACCGGAGA TGGCCGACGA CTACGACGAG 540 GCCTGGATGC TCAACACCGT GTTCGACTAT CACAACGAGA ACGCAAAAGA GGAGGTCATC 600 @@T@@@GTGT CCGACGTGAA CAAGGAGAGG GGGCCCATCG AA@T@@@AA@ TAAGGTAGA@ 66@ AAAGAGGGGA CCATCAGACT CGTCTACGAT GGGGAGCCCA CGTTTTCATA CAAGGAACAT @20 CCTAAGTTTT GA 732 !2) INFORMATIONS POUR LA SEQ ID NO: 3: (i) CARACTERISTIQUES DE LA SEQUENCE: (A) LONGUEUR: 243 acides amin s (B) TYPE: acide amin (C) NOMBRE DE BRINS: simple (D) CONFIGURATION: lin aire (ii) TYPE DE MOLECULE: peptide (xi) DESCRIPTION DE LA SEQUENCE: SEQ ID NO: 3: Met Pro Asn Phe Trp Ala Leu Pro Pro Glu Ile Asn Ser Thr Arg Ile 1 5 10 15 Tyr Leu Gly Pro Gly Ser Gly Pro Ile Leu Ala Ala Ala Gin Gly Trp 20 25 30 Asn Ala Leu Ala Ser Glu Leu Glu Lys Thr Lys Val Gly Leu Gln Ser 35 40 45 Ala eu Asp Thr Leu Leu Glu Ser Tyr Arg Gly Gln Ser Ser Gln Ala 50 55 oO Leu Ile Gln Gln Thr Leu Pro Tyr Val Gln Trp @@@ @@@ Thr Thr Ala 65 70 75 80 @@@ His Ala His Lys Thr Ala Ile Gln Leu Thr Ala Ala Ala Asn Ala 85 90 Tyr lu Gln Ala Arg Ala Ala Met Val Pro Pro Ala Met Val Arg Ala 100 105 110 Asn Arg Val Gln Thr Thr Val Leu Lys Ala Ile Asn Trp Phe Gly Gln 115 120 125 Phe Ser Thr Arg Ile Ala Asp Lys Glu Ala Asp Tyr Glu Gln Met Trp 130 135 140 Phe Gln Asp Ala Leu Val Met Glu Asn Tyr Trp Glu Ala Val Gln Glu 145 150 155 160 Ala Ile Gln Ser Thr Ser His Phe Glu Asp Pro Pro Glu Met Ala Asp 165 170 175 Asp Tyr Asp Glu Ala Trp Met Leu Asn Thr Val Phe Asp Tyr His Asn 180 185 190 Glu Asn Ala Lys Glu Glu Val Ile His Leu Val Pro Asp Val Asn Lys 195 200 205 Glu Arg Gly Pro Ile Glu Leu Val Thr Lys Val Asp Lys Glu Gly Thr 210 215 220 Ile Arg Leu Val Tyr Asp Gly Glu Pro Thr Phe Ser Tyr Lys Glu His 225 230 235 240 Pro Lys Phe