LISTE DES SEQUENCES SEQ ID n I : Polypeptide précurseur Gyr A de M. leprae SEQ ID n 2 : Séquence peptidique de t'intéine de M. leprae SEQ ID n 3 : Séquence peptidique de l'inteine de M. flavescens SEQ ID n 4 : Séquence peptidique de l'intéine de M. gordonae SEQ ID n 5 : Séquence peptidique de l'intéine de M. kansasii SEQ ID n 6 : Gène gyrA de M. leprae SEQ ID n 7 : Séquence nucléotidique de l'intéine de M. leprae SEQ ID n 8 : Séquence nucléotidique de l'intéine de M. flavescens SEQ ID n 9 : Séquence nucléotidique de l'intéine de M gordonae SEQ ID n 10 :Séquence nucléotidique de l'intéine de M. kansasfi SEQ ID n I Polypeptide precurseur Gyr A de M leprae 10 20 30 40 50 60 MTDITLPPGD GSIQRVEPVD IQQEMQRSYI DYAMSVIVGR ALPEVRDGLK PVHRRVLYAM 70 80 90 100 110 120 LDSGFRPDRS HAKSARSVAE TMGNYHPHGD ASIYDTLVRM AQPWSLRYPL VDGQGNFGSP 130 140 150 160 170 180 GNDPPAAMRY CVSGNSLVRL LFGKSIRIGD IVTGAQFNSD NPIDLKVLDR HGNPVVADYL 190 200 210 220 230 240 FHSGEHQTYT VRTTEGYETT GTSNHPLLCL VNVGGIPILL WKLIGETRSG DYVVLQRIPP 250 260 270 280 290 300 VEFGPADWYS TMEALLFGAF ISGGFVSQOH AGFNSLDRDY FIMVVNAYDT VVGGLRCISS 310 320 330 340 350 360 RITVSGSTLL ELDVYNLIEF KKIRLSGLOG QRSADKLVPD WLWHSPSTVK RAFLQALFEG 370 380 390 400 410 420 EGFSSILSRN IIELSYSTLS ERLAADVQQM LLEFGVVSER YCHTVNEYKV VIANRAQVEM 430 o 450 460 470 480 GVT KQAKLIRDVV SMSPCVGMDI NCVPGLATFI RKHCDNRWVE EDSFNQHNVD 490 500 510 520 530 540 CVQHWHHHSA EIVGHIADPD IRAIVTDL@D GRFYYARVAS VIDTGIQPVF SLHVDTEDHS 550 560 570 580 590 600 FL/INGFISHN TEARL/TPLAM EMLRETDEET VDFISNYDGR VQERMVLPSR FPNLLANGSG 610 620 630 640 650 660 GIAVGMATNI PPHNLYELAD AVFWCLENHD ADEETMLVAV MERVKGPDFP TAGLIVGSQG 670 680 690 700 710 720 IADAYKTGRG SIRIRGVVEV EEDSRGRTSL VTTELPYQVN HDNFTTSLAE QVRTGRLAGI 730 740 750 760 770 780 SNVEDQGSDR VGVRIVIEIK RDAVAKVVLN NLYKHTQLQT SFGANMLSIV DGVPRTLRLD 790 800 810 820 830 840 QMICYYVEHQ LDVIVRRTTY RLRKANERAH ILRGLVKALD ALDEVTTLIR ASQTVDLARV 850 860 870 880 890 900 GVVELLDIDO IQAQAILDMQ LRRLAALERQ RIIDDLAKTE VEIADLGDIL AKPERRRGII 910 920 930 940 950 960 RNELTEIAEK YGDDRRTRII AVDGDVNDED LIAREEVVVT ITETGYAKRT KTDLYRSQKR 970 980 990 1000 1010 1020 AGL KQDDIVRHFF VCSTHDWILF FTTQGRVYRA KAYELPEASR TARGQHVANL 1030 1040 1050 1060 1070 1080 LAFQPEERIA QVIQIRSYED APYLVLATRA GLVKKSKLTD FDSNRSGGIV AINLRDNDEL 1090 1100 1110 1120 1130 1140 VGAVLCAADG DLLLVSANGQ SIRFSATDEA LRPMGRATSG VQGMRFNADO RLLSLNVVRE 1150 1160 1 0 t180 1190 1200 DTYLLVATSG GYAKRTSIEE YRMQGRGGKG VLTVMYDRRR GSLVGAIVVD EDSELYATTS 1210 1220 1230 1240 1250 1260 GGGVIRTTAR QVRQAGRQIK GVRLMNLGEG DTLLAIARNA EESADGVSVK VMISRSRVLS 1270 FFGSDSNTSP DRT* SEQ ID n 2 :Séquence peptidique de l'intéine de M. leprae 1 CVSGNSLVRL LFGKSIRIGD IVTGAQFNSD NPIDLKVLDR HGNPVVADYL 51 FHSGEHQTYT VRTTEGYEIT GTSNHPLLCL VNVGGIPTLL WKLIGEIRSG 101 DYVVLQRIPP VEFGPADWYS TMEALLFGAF ISGGFVSQDH AGFNSLDRDY 151 FTMVVNAYDT VVGGLRCISS RITVSGSTLL ELDVYNLIEF KKTRLSGLGG 201 QRSADKLVPD WLWHSPSTVK RAFLQALFEG EGFSSILSRN IIEISYSTLS 251 ERLAADVQQMLLEFGVVSER YCHTVNEYKV VIANRAQVEM FFTQVGFGVT 301 KQAKLIRDVV SMSPCVGMDI NCVPGLATFI RKHCDNRWVE EDSFNQHNVD 351 CVQHWHHHSA EIVGHIADPD IRAIVTDLTD GRFYYARVAS VTDTGIQPVF 401 SLHVDTEDHS FLTNGFISHN SEQ ID n 3 :Séquence peptidique de l'intéine de M. flavescens I CVTGDALVRL PFGQSVRLRD WAGARSSSD NAIDLKVLNR HGDPWADKL 51 FHSGEHETYT VRTAEGYEVT GTANHPLLCL VDVGGVPTLL WKLTEEIRPG 101 DHVVLQRTPP TEFGPADWQD AFEALHLGAF ISEGFVSENR AGFNNLDREF 151 FNAVLTAYDT IVGGPRYVSS RTLASDSLLH ELDVHNLTAL KKSRLGEL VG 201 QRSADKAVPE WLWKAPAVVK RVFLQALFEG DGSCSALPRN TIQVSYSTRS 251 GRLAKDIQQM LLEFGVISRR YVHATGEHKV VLTSRAQAEL FAAQIGFGGI 301 KQAKLQGLLD ALPQAAAGRD GDYVPGLAQF VRKHSGSRWV DKDWLNRHNI 351 DRLSRWQRDG AEILGRIADP DVRAIAQELT DGRFYYARVA SVTDSGVQPV 401 YSLRVDTDDH SFTTNGFVSH N SEQ ID n 4 :Séquence peptidique de lintéine de M. gordonae I CLTGDALVRL PFGQSMRIGD VAPGARTNSD NAGELKVLDR HGDPVFADRL 51 FHSGDHQTFR VQTAEGYEVT GTSNHPVLCL VNLAGVPTLL WMLIEEIRPD 101 DYVVLQRAPP VESGPANWRD AMEALLLGAF ISEGFMSESR AGFNNVDRDY 151 FNAVVAAYDA VVGGKRYVAQ RTIASGSVLN ELDIHDVSAL KGTRLGVLCG 201 QRSADKSVPE WLWQSPAAVK RVFLQALFEG DGSCSALPRN TIQVSYSTRS 251 RQLAIDVQQM LLEFGVISRR YRHAVGEYKV VITNRAQAEL FATQIGFGGA 301 KQSKLTRILG SLPPCAGMDT NHVPGLAAFI RSHCDSEWVD KEWLRKHNID 351 RLSRWRRDGA EILSRIANPD VRAIATDLTD GRFYYAQVTS VTEAGVQPVY 401 SLRVDSEDHA FLTNGFVSHN SEQ ID n 5 Séquence peptidique de l'intéine de M. kansasii I CVTGDALVRL PFGQSMRIAD VVPGARPNSD NAVELKVLDR HGNPVAADRL 51 FHSGDHQTYM VRTAEGYEVT GTANHPLLCL VDVGGVPTLL WKLIEEIHPD 101 DWALQRTPP MELGPADWHD TMEALLLGAF ISEGCVSETR AGFANLDRDY 151 FTMVARAYDA VVGDKRDVYQ QTIASGSLQH TLYTQNVTAL KQSRLWQILG 201 MRSADTYVPE WMWHSPAAVK RVFLQALFEG DGSCSRRPHN TIQISYNTVS 251 KQLAMDVQQM LLEFGVISRR YLHAAGEYKV VITDRAQAEL FPKQIGFGGA 301 KQTELSKILA AMPPCAGRDS DHVPGLARFI RRHCDSRWVD KEWLHKHNID 351 HLSRWRRDGA EILSHIADPD VRTIATDLTD GRFYYARVAS VTDTGVQPVY 401 SLRVDTDDHA FLTNGFVSHN SEQ ID n 6 Gene gyrA de M lepr@e I ATGACTGATA TCACGCTGCC ACCAGGTGAC GGTTCTATAC AGCGGGTTGA 51 GCCGGTCGAC ATTCAGCAGG AAATGCAGCG CAGCTATATT GATTACGCGA 101 TGAGTGTGAT TGTGGGCCGG GCGTTGCCTG AAGTCCGCGA TGGTCTCAAA 151 CCGGTACATC GTCGGGTCTT GTACGCGATG TTAGACTCCG GTTTCCGCCC 201 GGACCGTAGC CACGCTAAGT CAGCACGGTC AGTCGCTGAG ACGATGGGCA 251 ATTACCATCC GCACGGCGAC GCATCGATTT ATGACACGTT AGTGCGCATG 301 GCGCAGCCGT GGTCGCTGCG GTATCCCTTG GTTGATGGGC AAGGCAATTT 351 CGGTTCGCCG GGTAATGACC CACCGGCAGC GATGCGTTAT TGTGTGTCAG 401 GAAATTCCTT GGTGAGGTTG CTATTTGGGA AATCAATACG AATCGGTGAT 451 ATCGITACTG GAGCTCAGTT CAATTCGGAC AATCCGATCG ACTTGAAGGT 501 TCTTGATCGG CATGGTAATC CGGTTGTAGC CGATTATTTA TTCCATTCAG 551 GAGAGCACCA AACCTATACA GTGCGCACCA CTGAAGGCTA TGAGATCACC 601 GGGACGTCGA ACCATCCCTT GTTGTGTTTA GTGAATGTCG GCGGTATACC 651 CACCTTGTTG TGGAAGCTGA TTGGAGAAAT TCGATCAGGA GACTACGTTG 701 TTTTACAGCG GATCCCACCA GTGGAATTTG GTCCGGCGGA CTGGTATTCT 751 ACGATGGAAG CATTGTTATT CGGAGCCTTT ATTAGTGGGG GCTTCGTTTC 801 TCAGGACCAT GCTGGATTTA ACAGCCTTGA CCGTGACTAT TTCACCATGG 851 TTGTTAATGC TTATGATACG GTTGTGGGTG GCCTGCGTTG CATATCTTCT 901 CGAATCACCG TATCGGGGTC GACGCTACTC GAACTTGATG TTTATAACCT 951 CATCGAGTTT AAGAAGACAA GACTTAGCGG TTTATGCGGG CAACGGTCTG 1001 CGGACAAGTT GGTACCTGAC TGGTTGTGGC ACTCACCTTC CACCGTCAAA 1051 CGAGCATTCC TTCAGGCATT GTTTGAAGGT GAAGGATTTT CTTCGATATT 1101 GTCGCGAAAT ATAATTGAGA TTTCCTACTC GACACTTAGT GAGCGACTGG 1151 CCGCCGACGT CCAGCAGATG CTGCTTGAAT TCGGAGTCGT GTCTGAGCGC 1201 TATTGCCATA CTGTCAATGA GTACAAGGTT GTCATAGCTA ACCGCGCTCA 1251 AGTAGAAATG TTTTTCACCC AAGTCGGTTT CGGTGTTACT AAACAAGCTA 1301 AGCTTATCCG GGACGTGGTA TCTATGTCTC CATGCGTTGG CATGGATATC 1351 AACTGCGTAC CAGGTTTGGC CACTTTCATT CGTAAGCATT GTGATAACCG 1401 CTGGGTCGAG GAAGACTCAT TTAATCAGCA TAATGTTGAT TGCGTCCAAC 1451 ATTGGCACCA TCATAGCGCG GAAATCGTCG GCCACATCGC CGATCCCGAT 1501 ATTCGTGCCA TCGTGACTGA CCTTACTGAT GGCCGGTTCT ACTACGCGCG 1551 CGTCGCGTCC GTGACTGATA CCGGTATTCA ACCTGTGTTC AGTCTACATG 1601 TGGACACCGA GGATCATTCG TTTTTGACTA ATGGATTCAT CAGCCATAAC 1651 ACCGAGGCTC GGCTTACTCC ATTGGCGATG GAAATGTTGC GCGAGATCGA 1701 CGAGGAGACA GTTGATTTCA TATCTAACTA CGATGGCCGG GTGCAGGAAC 1751 CGATGGTGTT GCCTAGCCGT TTTCCCAACC TGTTGGCTAA TGGTTCTGGC 1801 GGTATCGCGG TCGGCATGGC TACCAATATC CCGCCGCACA ACCTGTATGA 1851 GCTCGCCGAC GCTGTGTTTT GGTGCCTAGA GAACCATGAC GCTGACGAAG 1901 AGACGATGCT GGTCGCTGTT ATGGAACGGG TCAAAGGTCC TGATTTCCCT 1951 ACCGCCGGGT TGATTGTCGG TTCGCAAGGC ATTGCCGATG CTTACAAGAC 2001 TGGCCGTGGT TCCATTCGGA TACGCGGAGT TGTTGAGGTT GAAGAAGATT 2051 CACGCGGAAG GACGTCATTG GTCATCACTG AGCTACCGTA TCAGGTCAAC 2101 CACGACAACT TCATCACTTC TATCGCTGAG CAAGTCCGCA CTGGCCGGCT 2151 AGCCGGCATC TCCAATGTAG AAGACCAAGG CAGCGACCGG GTTGGTGTAC 2201 GTATCGTCAT CGAGATCAAG CGTGACGCGG TGGCCAAAGT GGTGCTCAAT 2251 AACCTGTACA AGCATACTCA GCTGCAAACT AGTTTCGGAG CCAACATGTT 2301 GTCAATCGTT GACGGCGTGC CGCGCACTTT GCGGTTGGAT CAGATGATTT 2351 GTTATTATGT CGAACATCAA CTGGACGTCA TTGTCCGGCG CACTACCTAC 2401 CGATTGCGTA AAGCCAACGA GCGGGCTCAT ATTTTGCGTG GATTGGTCAA 2451 AGCGCTCGAT GCGTTAGATG AGGTTATTAC GTTGATTCGG GCATCGCAGA 2501 CCGTGGATAT TGCTCGTGTT GGGGTGGTCG AGTTACTCGA TATCGACGAC 2551 ATTCAGGCTC AAGCTATCCT GGACATGCAG CTGCGGCGTT TGGCGGCTTT 2601 GGAGCGTCAA CGCATTATTG ATGATCTCGC TAAGATTGAG GTCGAGATCG 2651 CTGATCTGGG AGATATTCTG GCTAAGCCGG AGCGTCGGCG TGGTATCATT 2701 CGTAATGAAC TGACTGAGAT CGCAGAGAAG TACGGTGATG ACCGTCGTAC 2751 TCGGATAATA GCGGTTGATG GTGATGTCAA CGACGAGGAT TTGATTGCTC 2801 GTGAAGAGGT CGTTGTCACG ATAACTGAAA CTGGATATGC TAAACGTACT 2851 AAAACTGACC TGTATCGCAG CCAGAAACGC GGCGGGAAAG GTGTTCAAGG 2901 CGCCGGTTTG AAGCAGGACG ACATCGTCCG GCATTTCTTC GTGTGTTCAA 2951 CTCACGATTG GATCCTGTTT TTCACCACCC AAGGCCGCGT ATACCGGGCC 3001 AAGGCCTATG AATTGCCAGA GGCTTCTCGA ACGGCACGCG GGCAACACGT 3051 GGCCAATTTG CTTGCATTCC AGCCTGAAGA GCGCATCGCT CAGGTAATTC 3101 AGATCCGTAG CTATGAAGAC GCTCCATACT TGGTCCTTGC CACGCGCGCC 3151 GGTCTGGTTA AGAAGTCAAA GTTGACCGAT TTTGACTCTA ATCGTTCGGG 3201 TGGGATCGTG GCAATTAATT TACGTGACAA CGATGAGTTG GTCGGTGCAG 3251 TGTTGTGCGC GGCCGACGGC GACTTGCTTC TGGTATCGGC TAACGGCCAG 3301 TCTATCCGGT TCTCAGCGAC TGACGAGGCC TTGCGTCCGA TGGGGCGGGC 3351 TACCTCTGGT GTGCAGGGCA TGCGGTTTAA CGCCGATGAT CGACTGTTGT 3401 CGTTGAATGT GGTTCGCGAA GATACTTACC TGCTTGTCGC AACGTCTGGG 3451 GGTTACGCTA AACGCACCTC GATTGAGGAG TACCCGATGC AGGGCCGTGG 3501 CGGAAAGGGT GTTCTAACGG TCATGTACGA TCGTCGGCGC GGTAGCTTGG 3551 TTGGGGCCAT CGTGGTTGAT GAAGACAGCG AGTTGTACGC GATCACCTCA 3601 GGGGGTGGGG TAATTCGTAC AACGGCACGC CAGGTTCGCC AGGCAGGACG 3651 CCAGACCAAG GGTGTTCGGT TGATGAACTT AGGTGAGGGC GACACGCTGT 3701 TAGCCATCGC ACGTAATGCC GAAGAAAGCG CCGACGGCGT GTCGGTTAAA 3751 GTGATGATCT CAAGGAGTCG TGTACTTAGC TTTTTTGGTT CCGATAGCAA 3801 TACTTCGCCC GACCGGACAT GA SEQ ID n 7 :Séquence nucléotidique de l'intéine de M. leprae 1 TGTGTGTCAG GAAATTCCTT GGTGAGGTTG CTATTTGGGA AATCAATACG 51 AATCGGTGAT ATCGTTACTG GAGCTCAGTT CAATTCGGAC AATCCGATCG 101ACTTGAAGGT TCTTGATCGG CATGGTAATC CGGTTGTAGC CGATTATTTA 151TTCCATTCAG GAGAGCACCA AACCTATACA GTGCGCACCA CTGAAGGCTA 201TGAGATCACC GGGACGTCGA ACCATCCCTT GTTGTGTTTA GTGAATGTCG 251GCGGTATACC CACCTTGTTG TGGAAGCTGA TTGGAGAAAT TCGATCAGGA 301GACTACGTTG TTTTACAGCG GATCCCACCA GTGGAATTTG GTCCGGCGGA 351 CTGGTATTCT ACGATGGAAG CATTGTTATT CGGAGCCTTT ATTAGTGGGG 401 GCTTCGTTTC TCAGGACCAT GCTGGATTTA ACAGCCTTGA CCGTGACTAT 451 TTCACCATGG TTGTTAATGC TTATGATACG GTTGTGGGTG GCCTGCGTTG 501 CATATCTTCT CGAATCACCG TATCGGGGTC GACGCTACTC GAACTTGATG 551 TTTATAACCT CATCGAGTTT AAGAAGACAA GACTTAGCGG TTTATGCGGG 601 CAACGGTCTG CGGACAAGTT GGTACCTGAC TGGTTGTGGC ACTCACCTTC 651 CACCGTCAAA CGAGCATTCC TTCAGGCATT GTTTGAAGGT GAAGGATTTT 701 CTTCGATATT GTCGCGAAAT ATAATTGAGA TTTCCTACTC GACACTTAGT 751 GAGCGACTGG CCGCCGACGT CCAGCAGATG CTGCTTGAAT TCGGAGTCGT 801 GTCTGAGCGC TATTGCCATA CTGTCAATGA GTACAAGGTT GTCATAGCTA 851 ACCGCGCTCA AGTAGAAATG TTTTTCACCC AAGTCGGTTT CGGTGTTACT 901 AAACAAGCTA AGCTTATCCG GGACGTGGTA TCTATGTCTC CATGCGTTGG 951 CATGGATATC AACTGCGTAC CAGGTTTGGC CACTTTCATT CGTAAGCATT 1001 GTGATAACCG CTGGGTCGAG GAAGACTCAT TTAATCAGCA TAATGTTGAT 1051 TGCGTCCAAC ATTGGCACCA TCATAGCGCG GAAATCGTCG GCCACATCGC 1101 CGATCCCGAT ATTCGTGCCA TCGTGACTGA CCTTACTGAT GGCCGGTTCT 1151 ACTACGCGCG CGTCGCGTCC GTGACTGATA CCGGTATTCA ACCTGTGTTC 1201 AGTCTACATG TGGACACCGA GGATCATTCG TTTTTGACTA ATGGATTCAT 1251 CAGCCATAAC SEQ ID n 8 :Séquence nucléoudique de l'intéine de M. flavescens 1 TGCGTCACAG GTGATGCGCT GGTGCGCTTG CCATTCGGGC AATCCGTACG 51 GCTCCGTGAT GTCGTAGCGG GCGCGCGGTC GAGCTCGGAC AACGCCATTG 101 ATCTCAAGGT CCTCAACCGG CACGGGGACC CTGTGGTTGC CGACAAATTG 151 TTCCATTCCG GCGAACATGA GACATACACC GTCGCACCG CAGAAGGCTA 201 TGAGGTCACG GGCACTGCCA ACCACCCGTT GCTATGCCTG GTTGACGTGG 251 GTGGTGTGCC GACGCTGCTG TGGAAGCTGA CCGAAGAAAT TCGACCAGGC 301 GACCACGTGG TTCTCCAGCG CACCCCGCCG ACGGAGTTCG GTCCCGCGGA 351 CTGGCAGGAT GCTTTCGAGG CGCTACATCT CGGAGCCTTC ATCAGCGAGG 401 GTTTCGTATC GGAAAACCGT GCTGGCTTCA ACAATCTGGA CCGCGAGTTC 451 TTcAACGCCG TCCTGACTGC TTACGACACA ATTGTTGGCG GCCCCCGCTA 501 CGTATCGTCG CGCACCATCG CCTCGGACTC CCTACTCCAC GAACTCGACG 551 TACATAATTT GACGGCGCTG AAGAAGTCTC GACTCGGCGA GTTGGTTGGG 601 CAGCGCTCAG CCGACAAGGC GGTGCCGGAA TGGTTGTGGA AGGCACCGGC 651 TGTCGTCAAG CGAGTGTTCC TGCAGGCGCT GTTCGAGGGA GACGGATCGT 701 GTTCGCGTT GCCGAGGA"tC ACGATTCAGG TCTCGTATTC GACCCGCAGC 751 GGCGTCTTG CCAAGGACAT CCAGCAGATG CTGTTGGT TCGGGGTGAT 801 CTCGCGCCGC TACGTGCACG ACCGGGGA GCACAAGGTC GTGCTGACAA 851 GCAGGGCCCA GGCTGAACTG TTCGCTGCGC AGATCGGATT CGGCGGAATA 901 AAGCAGGCGA AGCTGCAGGG TCTATTGGAC GCACTTCCCC AGGCGGCGGC 951 CGGCCGGGAC GGCGACTACG TGCCGGGTCT CGCCCAATTC GTTCGTAAGC 1001 ACAGCGGCAG CCGCTGGGTC GACAAGGACT GGCTGAACAG ACACAACATC 1051 GACCGGCTGT CTCGCTGGCA ACGCGATGGT GCTGAGATTC TCGGCCGCAT 1101 CGCCGATCCC GACGTCCGGG CAATCGCTCA GGAACTCACC GACGGCCGGT 1151 TCTACTACGC GCGTGTGGCA TCCGTGACTG ATAGCGGTGT GCAACCGGTG 1201 TACAGCCTGA GGGTCGACAC CGATGACCAC TCGTTCATCA CAAACGGCTT 1251 CGTCAGCCAC MC SEQ ID n 9 : Séquence nucléotidique de l'intéine dc M gordonae 1 TGTCTCACCG GAGATGCATT GGTGCGCTTA CCATTTGGAC AGTCGATGCG 51 MTCGGCGATGTCGCTCCGGGGGCTCGGAC AAATTCGGAC MCGCCGGCG 101 AGTTGAAGGT CCTCGATAGG CACGGTGACC CGGTTTTCGC TGATCGCTTG 151 TTCCATTCGG GTGATCACCA GACnTCAGG GTGCAAACTG CCGAGGGCTA 201 TGAGGTCACA GGCACUCGA ATCACCCTGT GCTHGCCTG GTGMCCTCG 251 CCGGCGTGCC GACACTCCTG TGGATGTTGA TCGAGGAGAT TCGACCAGAC 301 GACTACGTCG TCTTACAGCG CGCGCCGCCT GTTGAATCAG GTCCGGCGAA 351 CTGGCGCGAC GcCATGGAGG CACTGTTACT CGGGGCCTTC ATCAGCGMG 401 GGTTCATGTC CGAGTCTCGA GCTGGTrTTA ACMCGTCGA CCGCGACTAC 451 TTCAACGCGG TGGTAGCGGC CTACGACGCG GTTGTCGGAG GCAAGCGGTA 501 CGTAGCTCAA CGGACTATCG CGTCGGGTTC TGTGCTGAAC GAGTTGGATA 551 TTCACGATGT TTCAGCTTTG AAGGGCACTC GGCTCGGTGT GCTCTGCGGG 601 CAACGCTCCG CAGACAAATC GGTGCCCGAG TGGCTTTGGC MTCCCCCGC 651 CGCOGTGAAG CGGGTATTCC TGCAGGCGTT GTTCGAGGGT GATGGCTCGT 701 GTTCGGCGTT ACCCCGGAAC ACMTTCAGG TTTCTTATTC GACACGTAGT 751 AGACAGCTTG ccATCGATGT GCMCAGATG CTGCTCGAGT TCGGAGTCAT 801 ATCGcGCCGG TACCGCCATG CCGTCGGTGA GTATMGGTC GTCATMCCA 851 ACCGTGCTCA GGCCGAGCTT TTCGCCACTC AAATCGGTTT CGGCGGCGCC 901 AAACAAAGTA AGCTGACCAG GATCCTGGGT TCACTGCCGC CCTGCGCCGG 951 CATGGACACC AACCACGTTC CCGGCCTGGC CGCTtTCATC CGCAGCCATT 1001 GCGATAGCGA ATGGGTAGAT AAGGAGTGGC TGCGTAAGCA CAACATCGAT 1051 CGGCTGTCCC GGTGGCGTCG CGACGGTGCG GAAATACTCT CGCGCATCGC 1101 GAACCCCGAT GTTCGTGCCA TCGCMcCGA CCTCAcCGAC GGGCGCTTCT 1151 ACTACGCCCA AGTMCCTCG GTCACAGAGG CGGGCGTGCA GCCGGTGTAC I 201 AGCCTGCGCG TTGACTCCGA GTCACGCC TTCCTGACCA ACGGATTCGT 1251 CAGCCACAAC SEQ ID n 10 : séquence nucléotidique dc l'intéine dc M. kansasil 1 TGCGTGACCG GAGATGCGTT GGTGCGGTTG CCATGGAC AGTCGATGCG 51 CATCGCCGAT GTCGTGCCGG GCGCCCGGCC CAATTCGGAT AACGCGGTCG 101 AGCTGAAGGT GCTGGATCGG CACGGCAACC CGGTAGCGGC CGATCGCCTC 151 TTTCATTCCG GTGACCATCA GACCTACATG GTGCGCACCG CGGAGGGTTA 201 TGAGGTCACC GGCACCGCGA ACCACCCGTT GTTGTGCCTT GTCGACGTTG 251 GTGGCGTGCC CACGUGTTG TGGMGCTGA TCGMGAAA CCACCCAGAC 301 GATTATGTGG CGCTACAGCG CACCCCTCCA ATGGAATTGG GCCCCGCTGA 351 CTGGCACGAC ACGATGGAAG CGCTCCTGTT GGGGGCATTC ATTAGTGAGG 401 GCTGTGHTC GGAMCCCGC GCCGGCTTTG CCAATCTGGA CCGCGACTAC 451 TTCACCATGG TGGCCCGGGC TTACGACGCC GTTGTCGGCG ACAAACGCGA 501 TGTCTACCAG CAGACAATCG CCTCGGGATC GCTGCAGCAC ACGCTCTACA 551 CCCAGAACGT GACTGCTCTG AAGCAGAGCC GGTTGTGGCA GATACTGGGC 601 ATGCGGTCGG CAGACACGTA TGTGCCGGAG TGGATGTGGC ACTCTCCCGC 651 GGCCGTCAAA CGCGTCTTCC TGCAAGCACT CTTCGAGGGC GACGGTTCGT 701 GTTCGAGGCG GCCACACAAT ACGATTCAGA TCTCATACAA CACCGTAAGC 751 AAGCAGCTGG CCATGGACGT GCAGCAGATG TTGCTCGAGT TCGGTGTGAT 801 ATCCCGGCGA TACTTACATG CGGCCGGCGA GTACAAGGTC GTCATCACCG 851 ATCGGGCGCA AGCCGAATTA TTTCCAAAGC AAATCGGTTT CGGTGGCGCA 901 AAGCAAACCG AGCTGAGCAA GATCCTGGCC GCCATGCCCC CGTGCGCCGG 951 CAGAGACAGC GATCACGTGC CCGGACTAGC TCGGrtTA CGGAGGCACT 1001 GCGACAGCCG GTGGGTCGAC AAGGAATGGT TGCACAAGCA CAACATTGAC 1051 CACCTnCGC GGTGGCGCCG CGACGGTGCC GAAATcCTCT CGCACATCGC 1101 CGACCCCGAT GTCCGTACCA TTGCGACGGA CCTCACCGAC GGCCGCTTCT 1151 ACTACGCGCG GGTCGCCTCC GTCACGGACA CCGGCGTGCA ACCGGTGTAC iM1 AGCCTGCGGG TCGACACCGA CGACCACGCC TTCCTCACCA ACGGTTTCGT 1251 CAGCCACAAC BIBLIOGRAPHIE 1. Hirata, R., Ohsumi, Y., Nakano, A., Kawasaki, H., Suzuki, K. & BR Anraku, Y (1990) J. Bio. Chem. 265,67266733.