LISTE DE SEQUENCES (1) INFORMATIONS GENERALES: (i) DEPOSANT: (A) NOM: ASSOCIATION POUR LE DEVELOPPEMENT DE LA RECHERCHE GENETIQUE MOLECULAIRE (B) RUE: 26 RUE MONTPENSIER (C) VILLE: PARIS (E) PAYS: FRANCE (F) CODE POSTAL: 751 (ii) TITRE DE L' INVENTION: "RECEPIEUR NUCLEAIRE DE GLUCOCORTICOIDES MODIFIE, PROTEINE DE FUSION, FRAGMENTS D'ADN CODANT POUR LEDIT RECEPTEUR ET LADITE PROTEINE DE FUSION,VECTEURS, CELLULES ET ANIMAUX EN COMPRENANT ET PROCEDES DANS LESQUELS ILS SONT MIS EN OEUVRE" (iii) NOMBRE DE SEQUENCES: 3 (iv) FORME DECHIFFRABLE PAR ORDINATEUR: (A) TYPE DE SUPPORT: Floppy disk (B) ORDINATEUR: IBM PC compatible (C) SYSTEME D' EXPLOITATION: PC-DOS/MS-DOS (D) LOGICIEL:PatentIn Release #i.e, Version #1.3 (OEB) (2) INFORMATIONS POUR LA SEQ ID NO: 1: (i) CARACTERISTIQUES DE LA SEQUENCE: (A) LONGUEUR: 2334 nucléotides (B) TYPE: nucléotide (C) NOMBRE DE BRINS: simple (D) CONFIGURATION: linéaire (ii) TYPE DE MOLECULE: ADNc (ix) CARACTERISTIQUE: (A) NOM/CLE: GR(I747/T) (Récepteur humain des glucocorticoïdes muté en position 747) (B) EMPLACEMENT:1..2331 (i) CARACTERISTIQUES DE LA SEQUENCE: (A) LONGUEUR: 777 acides aminés (B) TYPE: acide aminé (D) CONFIGURATION: linéaire (ii) TYPE DE MOLECULE: protéine (xi) DESCRIPTION DE LA SEQUENCE:SEQ ID NO: 1: ATG GAC TCC AAA GAA TCA TTA ACT CCT GGT AGA GAA GAA AAC CCC AGC 48 Met Asp Ser Lys Glu Ser Leu Thr Pro Gly Arg Glu Glu Asn Pro Ser 1 5 10 15 AGT GTG CTT GCT CAG GAG AGG GGA GAT GTG ATG GAC TTC TAT AAA ACC 96 Ser Val Leu Ala Gin Glu Arg Gly Asp Val Met Asp Phe Tyr Lys Thr 20 25 30 CTA AGA GGA GGA GCT ACT GTG AAG GTT TCT GCG TCT TCA CCC TCA CTG 144 Leu Arg Gly Gly Ala Thr Val Lys Val Ser Ala Ser Ser Pro Ser Leu 35 40 45 GCT GTC GCT TCT CAA TCA GAC TCC AAG CAG CGA AGA CTT TTG GTT GAT 192 Ala Val Ala Ser Gln Ser Asp Ser Lys Gln Arg Arg Leu Leu Val Asp 50 55 60 UT CCA AAA GGC TCA GTA AGC AAT GCG CAG CAG CCA GAT CTG TCC AAA 240 Phe Pro Lys Gly Ser Val Ser Asn Ala Gln Gin Pro Asp Leu Ser Lys 65 70 75 80 GCA GTT TCA CTC TCA ATG GGA CTG TAT ATG GGA GAG ACA GAA ACA AAA 288 Ala Val Ser Leu Ser Met Gly Leu Tyr Met Gly Glu Thr Glu Thr Lys 85 90 95 GTG ATG GGA AAT GAC CTG GGA UC CCA CAG CAG GGC CAA ATC AGC CTT 336 Val Met Gly Asn Asp Leu Gly Phe Pro Gln Gln Gly Gln Ile Ser Leu 100 105 118 TCC TCG GGG GAA ACA GAC TTA AAG CTT TTG GAA GAA AGC ATT GCA AAC 384 Ser Ser Gly Glu Thr Asp Leu Lys Leu Leu Glu Glu Ser Ile Ala Asn 115 120 125 CTC AAT AGG TCG ACC AGT GTT CCA GAG AAC CCC AAG AGT TCA GCA TCC 432 Leu Asn Arg Ser Thr Ser Val Pro Glu Asn Pro Lys Ser Ser Ala Ser 130 135 140 ACT GCT GTG TCT GCT GCC CCC ACA GAG AAG GAG UT CCA AAA ACT CAC 480 Thr Ala Val Ser Ala Ala Pro Thr Glu Lys Glu Phe Pro Lys Thr His 145 150 155 160 TCT GAT GTA TCT TCA GAA CAG CAA CAT TTG AAG GGC CAG ACT GGC ACC 528 Ser Asp Val Ser Ser Glu Gln Gln His Leu Lys Gly Gln Thr Gly Thr 165 170 175 AAC GGT GGC AAT GTG AAA TTG TAT ACC ACA GAC CAA AGC ACC UT GAC 576 Asn Gly Gly Asn Val Lys Leu Tyr Thr Thr Asp Gln Ser Thr Phe Asp 180 185 190 ATT TTG CAG GAT TTG GAG UT TCT TCT CGC TCC CCA GGT AAA GAG ACG 624 Ile Leu Gin Asp Leu Glu Phe Ser Ser Gly Ser Pro Gly Lys Glu Thr 195 200 205 AAT GAG AGT CCT TGG AGA TCA GAC CTC TTG ATA GAT GAA AAC TGT TTG 672 Asn Glu Ser Pro Trp Arg Ser Asp Leu Leu Ile Asp Glu Asn Cys Leu 210 215 220 CTT TCT CCT CTG CCC GGA GAA GAC GAT TCA TTC CTT TTG GAA GGA AAC 720 Leu Ser Pro Leu Ala Gly Glu Asp Asp Ser Phe Leu Leu Glu Gly Asn 225 230 235 240 TCG AAT GAG GAC TGC AAG CCT CTC ATT TTA CCG GAC ACT AAA CCC AAA 768 Ser Asn Glu Asp Cys Lys Pro Leu Ile Leu Pro Asp Thr Lys Pro Lys 245 250 255 ATT AAC GAT AAT GGA GAT CTC GTT TTG TCA AGC CCC AGT AAT GTA ACA 816 Ile Lys Asp Asn Gly Asp Leu Val Leu Ser Ser Pro Ser Asn Val Thr 260 265 270 CTG CCC CAA CTC AAA ACA GAA AAA GAA GAT TTC ATC GAA CTC TGC ACC 864 Leu Pro Gln Val Lys Thr Glu Lys Glu Asp Phe Ile Glu Leu Cys Thr 275 280 285 CCT CGC GTA ATT AAG CAA GAG AAA CTG CCC ACA GTT TAC TGT CAG GCA 912 Pro Gly Val Ile Lys Gln Glu Lys Leu Gly Thr Val Tyr Cys Gln Ala 290 295 300 AGC TTT CCT GGA GCA AAT ATA ATT GGT AAT AAA ATC TCT GCC ATT TCT 960 Ser Phe Pro Gly Ala Asn Ile Ile Gly Asn Lys Met Ser Ala Ile Ser 305 310 315 320 GTT CAT GGT GTC AGT ACC TCT GGA GGA CAG ATG TAC CAC TAT GAC ATG 1008 Val His Gly Val Ser Thr Ser Gly Gly Gln Met Tyr His Tyr Asp Met 325 330 335 AAT ACA GCA TCC CTT TCT CAA CAG CAG GAT CAG AAG CCT ATT UT AAT 1056 Asn Thr Ala Ser Leu Ser Gln Gln Gln Asp Gln Lys Pro Ile Phe Asn 340 345 350 GTC ATT CCA CCA ATT CCC GTT GGT TCC GAA AAT TGG AAT AGG TGC CAA 1104 Val Ile Pro Pro Ile Pro Val Gly Ser Glu Asn Trp Asn Arg Cys Gln 355 360 365 GGA TCT GGA GAT GAC AAC TTG ACT TCT CTC GGG ACT CTC AAC TTC CCT 1152 Gly Ser Gly Asp Asp Asn Leu Thr Ser Leu Gly Thr Leu Asn Phe Pro 370 375 380 GGT CGA ACA GTT UT TCT AAT GGC TAT TCA AGC CCC AGC ATG AGA CCA 1200 Gly Arg Thr Val Phe Ser Asn Gly Tyr Ser Ser Pro Ser Met Arg Pro 385 390 395 400 GAT GTA AGC TCT CCT CCA TCC AGC TCC TCA ACA GCA ACA ACA GGA CCA 1248 Asp Val Ser Ser Pro Pro Ser Ser Ser Ser Thr Ala Thr Thr Gly Pro 405 410 415 CCT CCC AAA CTC TGC CTG GTG TGC TCT GAT GAA GCT TCA GGA TGT CAT 1296 Pro Pro Lys Leu Cys Leu Val Cys Ser Asp Glu Ala Ser Gly Cys His 420 425 430 TAT GGA GTC TTA ACT TGT GGA AGC TGT AAA GTT TTC TTC AAA ACA GCA 1344 Tyr Gly Val Leu Thr Cys Gly Ser Cys Lys Val Phe Phe Lys Arg Ala 435 440 445 GTG GAA GGA CAG CAC AAT TAC CTA TGT GCT GGA AGG AAT GAT TGC ATC 1392 Val Glu Gly Gln His Asn Tyr Leu Cys Ala Gly Arg Asn Asp Cys île 450 455 460 ATC GAT AAA ATT CGA AGA AAA AAC TGC CCA GCA TGC CGC TAT CGA AAA 1440 île Asp Lys Ile Arg Arg Lys Asn Cys Pro Ala Cys Arg Tyr Arg Lys 465 470 475 480 TGT CTT CAG GCT GGA ATG AAC CTG GAA GCT CGA AAA ACA AAG AAA AAA 1488 Cys Leu Gln Ala Gly Met Asn Leu Glu Ala Arg Lys Thr Lys Lys Lys 485 490 495 ATA AAA GGA ATT CAG CAG GCC ACT ACA GGA GTC TCA CAA GAA ACC TCT 1536 Ile Lys Gly Ile Gln Gln Ala Thr Thr Gly Val Ser Gln Glu Thr Ser 500 505 510 GAA AAT CCT GGT AAC AAA ACA ATA GTT CCT GCA ACC TTA CCA CAA CTC 1584 Glu Asn Pro Gly Asn Lys Thr Ile Val Pro Ala Thr Leu Pro Gln Leu 515 520 525 ACC CCT ACC CTC GTC TCA CTG TTG GAG GTT ATT GAA CCT CAA GTG TTA 1632 Thr Pro Thr Leu Val Ser Leu Leu Glu Val Ile Glu Pro Glu Val Leu 530 535 540 TAT GCA GGA TAT GAT AGC TCT GTT CCA GAC TCA ACT TGC ACC ATC ATC 1680 Tyr Ala Gly Tyr Asp Ser Ser Val Pro Asp Ser Thr Trp Arg Ile Met 545 550 555 560 ACT ACC CTC AAC ATG TTA GGA CGC CGC CAA CTC ATT GCA GCA GTG AAA 1728 Thr Thr Leu Asn Met Leu Gly Gly Arg Gln Val Ile Ala Ala Val Lys 565 570 575 TGG GCA AAG GCA ATA CCA GGT UC AGG AAC TTA CAC CTG GAT GAC CAA 1776 Trp Ala Lys Ala Ile Pro Gly Phe Arg Asn Leu His Leu Asp Asp Gln 580 585 590 ATG ACC CTA CTG CAG TAC TCC TGG ATG UT CTT ATG GCA UT GCT CTG 1824 Met Thr Leu Leu Gln Tyr Ser Trp Met Phe Leu Met Ala Phe Ala Leu 595 600 605 GGG TGG AGA TCA TAT AGA CAA TCA AGT GCA AAC CTG CTG TGT UT GCT 1872 Gly Trp Arg Ser Tyr Arg Gln Ser Ser Ala Asn Leu Leu Cys Phe Ala 610 615 620 CCT GAT CTG AH ATT AAT GAG CAG AGA ATG ACT CTA CCC TGC ATG TAC 192 Pro Asp Leu Ile Ile Asn Glu Gln Arg Met Thr Leu Pro Cys Met Tyr 625 63 635 640 GAC CAA TGT AAA CAC ATG CTG TAT GTT TCC TCT GAG TTA CAC AGG CTT 1968 Asp Gln Cys Lys His Met Leu Tyr Val Ser Ser Glu Leu His Arg Leu 645 650 655 CAG GTA TCT TAT GAA GAG TAT CTC TGT ATG AAA ACC TTA CTG CTT CTC 2016 Gln Val Ser Tyr Glu Glu Tyr Leu Cys Met Lys Thr Leu Leu Leu Leu 660 665 670 TCT TCA GTT CCT AAG GAC GGT CTG AAG AGC CAA GAG CTA UT GAT GAA 2064 Ser Ser Val Pro Lys Asp Gly Leu Lys Ser Gln Glu Leu Phe Asp Glu 675 680 685 AH AGA ATG ACC TAC ATC AAA GAG CTA GGA AAA GCC AU GTC AAG AGG 2112 Ile Arg Met Thr Tyr Ile Lys Glu Leu Gly Lys Ala Ile Val Lys Arg 690 695 700 GAA GGA AAC TCC AGC CAG AAC TGG CAG CGG UT TAT CAA CTG ACA AAA 2160 Glu Gly Asn Ser Ser Gln Asn Trp Gln Arg Phe Tyr Gln Leu Thr Lys 705 710 715 720 CTC TTG GAT TCT ATG CAT GAA GTG GTT GAA AAT CTC CTT AAC TAT TGC 2208 Leu Leu Asp Ser Met His Glu Val Val Glu Asn Leu Leu Asn Tyr Cys 725 730 735 TTC CAA ACA UT TTG GAT AAG ACC ATG TCC ACC GAG TTC CCC GAG ATG 2256 Phe Gln Thr Phe Leu Asp Lys Thr Met Ser Thr Glu Phe Pro Glu Met 740 745 750 HA GCT GAA ATC ATC ACC AAT CAG ATA CCA AAA TAT TCA AAT GGA AAT 2304 Leu Ala Glu Ile Ile Thr Asn Gln Ile Pro Lys Tyr Ser Asn Gly Asn 755 760 765 ATC AAA AAA CTT CTG UT CAT CAA AAG TGA 2334 Ile Lys Lys Leu Leu Phe His Gln Lys 770 775 (2) INFORMATIONS POUR LA SEQ ID NO: 2: (i) CARACTERISTIQUES DE LA SEQUENCE: (A) LONGUEUR: 2004 nucléotides (B) TYPE: nucléotide (C) NOMBRE DE BRINS: simple (D) CONFIGURATION: linéaire (ii) TYPE DE MOLECULE: ADN de phage et ADNc (ix) CARACTERISTIQUE: (A) NOM/CLE: CRE-LBD[GR(I747/T)] protéine de fusion du domaine LBD du récepteur humain des glucocorticoldes muté en position 747 et de la protéine recombinase Cre (B) EMPLACEMENT:1..2001 (i) CARACTERISTIQUES DE LA SEQUENCE: (A) LONGUEUR: 667 acides aminés (B) TYPE: acide aminé (D) CONFIGURATION: linéaire (ii) TYPE DE MOLECULE: protéine (xi) DESCRIPTION DE LA SEQUENCE:SEQ ID NO: 2: ATG TCC AAT TTA CTG ACC GTA CAC CAA AAT TTG CCT GCA TTA CCG GTC 48 Met Ser Asn Leu Leu Thr Val His Gîn Asn Leu Pro Ala Leu Pro Val 1 5 10 15 GAT GCA ACG AGT GAT GAG GTT CGC AAG AAC CTG ATG GAC ATG HC AGG 96 Asp Ala Thr Ser Asp Glu Val Arg Lys Asn Leu Met Asp Met Phe Arg 20 25 30 GAT CGC CAG GCG UT TCT GAG CAT ACC TGG AAA ATG CTT CTG TCC GTT 144 Asp Arg Gln Ala Phe Ser Glu His Thr Trp Lys Met Leu Leu Ser Val 35 40 45 TGC CGG TCG TGG GCG GCA TGG TGC AAG TTG AAT AAC CGG AAA TGG UT 192 Cys Arg Ser Trp Ala Ala Trp Cys Lys Leu Asn Asn Arg Lys Trp Phe 50 55 60 CCC GCA GAA CCT GAA GAT GTT CGC GAT TAT CH CTA TAT CTT CAG GCG 240 Pro Ala Glu Pro Glu Asp Val Arg Asp Tyr Leu Leu Tyr Leu Gîn Ala 65 70 75 80 CGC GGT CTG GCA GTA AAA ACT ATC CAG CAA CAT TTG GGC CAG CTA AAC 288 Arg Gly Leu Ala Val Lys Thr Ile Gln Gln His Leu Gly Gln Leu Asn 85 9 95 ATG CTT CAT CGT CGG TCC GGG CTG CCA CGA CCA AGT GAC AGC AAT GCT 336 Met Leu His Arg Arg Ser Gly Leu Pro Arg Pro Ser Asp Ser Asn Ala 100 105 110 GTT TCA CTG GTT ATG CGG CGG ATC CGA AAA GAA AAC GTT GAT GCC GGT 384 Val Ser Leu Val Met Arg Arg Ile Arg Lys Glu Asn Val Asp Ala Gly 115 120 125 GAA CGT GCA AAA CAG GCT CTA GCG HC GAA CGC ACT GAT TTC GAC CAG 432 Glu Arg Ala Lys Gln Ala Leu Ala Phe Glu Arg Thr Asp Phe Asp Gln 130 135 140 GTT CGT TCA CTC ATG GAA AAT AGC GAT CGC TGC CAG GAT ATA CGT AAT 480 Val Arg Ser Leu Met Glu Asn Ser Asp Arg Cys Gln Asp Ile Arg Asn 145 15 155 160 CTG GCA UT CTG GGG ATT GCT TAT AAC ACC CTG TTA CGT ATA GCC GAA 528 Leu Ala Phe Leu Gly Ile Ala Tyr Asn Thr Leu Leu Arg Ile Ala Glu 165 170 175 ATT GCC AGG ATC AGG GTT AAA GAT ATC TCA CGT ACT GAC GGT GGG AGA 576 Ile Ala Arg Ile Arg Val Lys Asp Ile Ser Arg Thr Asp Gly Gly Arg 180 185 190 ATG TTA ATC CAT ATT GGC AGA ACG AAA ACG CTG GTT AGC ACC GCA GGT 624 Met Leu Ile His Ile Gly Arg Thr Lys Thr Leu Val Ser Thr Ala Gly 195 200 205 GTA GAG AAG GCA CTT AGC CTG GGG GTA ACT AAA CTG GTC GAG CGA TGG 672 Val Glu Lys Ala Leu Ser Leu Gly Val Thr Lys Leu Val Glu Arg Trp 210 215 220 AH TCC GTC TCT GGT GTA GCT GAT GAT CCG AAT AAC TAC CTG UT TGC 720 Ile Ser Val Ser Gly Val Ala Asp Asp Pro Asn Asn Tyr Leu Phe Cys 225 230 235 240 CGG GTC AGA AAA AAT GGT GTT GCC GCG CCA TCT GCC ACC AGC CAG CTA 768 Arg Val Arg Lys Asn Gly Val Ala Ala Pro Ser Ala Thr Ser Gln Leu 245 250 255 TCA ACT CGC GCC CTG GAA GGG ATT UT GAA GCA ACT CAT CGA TTG ATT 816 Ser Thr Arg Ala Leu Glu Gly Ile Phe Glu Ala Thr His Arg Leu Ile 260 265 270 TAC GGC GCT AAG GAT GAC TCT GGT CAG AGA TAC CTG GCC TGG TCT GGA 864 Tyr Gly Ala Lys Asp Asp Ser Gly Gln Arg Tyr Leu Ala Trp Ser Gly 275 280 285 CAC AGT GCC CGT GTC GGA GCC GCG CGA GAT ATG GCC CGC GCT GGA GTT 912 His Ser Ala Arg Val Gly Ala Ala Arg Asp Met Ala Arg Ala Gly Val 290 295 300 TCA ATA CCG GAG ATC ATG CAA GCT GGT GGC TGG ACC AAT GTA AAT ATT 960 Ser Ile Pro Glu Ile Met Gln Ala Gly Gly Trp Thr Asn Val Asn Ile 305 310 315 320 GTC ATG AAC TAT ATC CGT AAC CTG GAT AGT GAA ACA GGG GCA ATC GTG 1008 Val Met Asn Tyr Ile Arg Asn Leu Asp Ser Glu Thr Gly Ala Met Val 325 330 335 CCC CTC CTG GAA GAT CCC GAT CTC GAG ATT CAG CAG GCC ACT ACA GGA 1056 Arg Leu Leu Glu Asp Gly Asp Leu Glu Ile Gln Gln Ala Thr Thr Gly 340 345 350 CTC TCA CAA GAA ACC TCT GAA AAT CCT GGT AAC AAA ACA ATA GTT CCT 1104 Val Ser Gln Glu Thr Ser Glu Asn Pro Gly Asn Lys Thr Ile Val Pro 355 360 365 CCA ACG TTA CCA CAA CTC ACC CCT ACC CTC GTG TCA CTG TTG GAG GTT 1152 Ala Thr Leu Pro Gln Leu Thr Pro Thr Leu Val Ser Leu Leu Glu Val 370 375 380 ATT GAA CCT GAA GTG TTA TAT GCA GGA TAT GAT AGC TCT GTT CCA GAC 1200 Ile Glu Pro Glu Val Leu Tyr Ala Gly Tyr Asp Ser Ser Val Pro Asp 385 390 395 400 TCA ACT TGC AGG ATC ATG ACT ACC CTC AAC ATC TTA GGA GGG CGG CAA 1248 Ser Thr Trp Arg Ile Met Thr Thr Leu Asn Met Leu Gly Gly Arg Gln 405 410 415 GTC ATT GCA GCA GTG AAA TGG GCA AAC GCA ATA CCA GGT TTC AGG AAC 1296 Val Ile Ala Ala Val Lys Trp Ala Lys Ala Ile Pro Gly Phe Arg Asn 420 425 430 TTA CAC CTC GAT GAC CAA ATG ACC CTA CTC CAG TAC TCC TGG ATC UT 1344 Leu His Leu Asp Asp Gln Met Thr Leu Leu Gln Tyr Ser Trp Met Phe 435 440 445 CTT ATC GCA TTT GCT CTC GGG TGG AGA TCA TAT AGA CAA TCA AGT GCA 1392 Leu Met Ala Phe Ala Leu Gly Trp Arg Ser Tyr Arg Gin Ser Ser Ala 450 455 460 AAC CTC CTC TGT UT GCT CCT GAT CTC ATT ATT AAT GAG CAG AGA ATC 1440 Asn Leu Leu Cys Phe Ala Pro Asp Leu Ile Ile Asn Glu Gln Arg Met 465 470 475 480 ACT CTA CCC TGC ATC TAC GAC CAA TCT AAA CAC ATC CTG TAT GTT TCC 1488 Thr Leu Pro Cys Met Tyr Asp Gln Cys Lys His Met Leu Tyr Val Ser 485 49 495 TCT GAG TTA CAC AGG CTT CAG GTA TCT TAT GAA GAG TAT CTC TCT ATC 1536 Ser Glu Leu His Arg Leu Gln Val Ser Tyr Glu Glu Tyr Leu Cys Met 500 505 510 AAA ACC TTA CTC CTT CTC TCT TCA GTT CCT AAG GAC GGT CTG AAG AGC 1584 Lys Thr Leu Leu Leu Leu Ser Ser Val Pro Lys Asp Gly Leu Lys Ser 515 520 525 CAA GAG CTA UT GAT GAA ATT AGA ATC ACC TAC ATC AAA GAG CTA GGA 1632 Gln Glu Leu Phe Asp Glu Ile Arg Met Thr Tyr Ile Lys Glu Leu Gly 530 535 540 AAA GCC ATT GTC AAC AGG GAA GGA AAC TCC AGC CAG AAC TGC CAG CGC 168 Lys Ala Ile Val Lys Arg Glu Gly Asn Ser Ser Gln Asn Trp Gln Arg 545 550 555 560 UT TAT CAA CTG ACA AAA CTC TTG GAT TCT ATC CAT GAA GTG GTT GAA 1728 Phe Tyr Gln Leu Thr Lys Leu Leu Asp Ser Met His Glu Val Val Glu 565 570 575 AAT CTC CTT AAC TAT TGC TTC CAA ACA TTT TTG GAT AAC ACC ATC TCC 1776 Asn Leu Leu Asn Tyr Cys Phe Gln Thr Phe Leu Asp Lys Thr Met Ser 58 585 59 ACC GAG TTC CCC GAG ATC TTA GCT GAA ATC ATC ACC AAT CAG ATA CCA 1824 Thr Glu Phe Pro Glu Met Leu Ala Glu Ile Ile Thr Asn Gln Ile Pro 595 600 605 AAA TAT TCA AAT GGA AAT ATC AAA AAA CTT CTG UT CAT CAA AAG GGT 1872 Lys Tyr Ser Asn Gly Asn Ile Lys Lys Leu Leu Phe His Gln Lys Gly 610 615 620 ACC AGC CGT GGA CGC GCA TCC GTG GAG GAG ACG GAC CAA AGC CAC TTG 1920 Thr Ser Arg Gly Gly Ala Ser Val Glu Glu Thr Asp Gln Ser His Leu 625 63 635 64 GCC ACT CCC GGC TCT ACT TCA TCG CAT TCC TTG CAA AAG TAT TAC ATC 1968 Ala Thr Ala Gly Ser Thr Ser Ser His Ser Leu Gln Lys Tyr Tyr Ile 645 65 655 ACC GGG GAG GCA GAG GGT TTC CCT GCC ACA GTC TGA 2004 Thr Gly Glu Ala Glu Gly Phe Pro Ala Thr Val 660 665 (2) INFORMATIONS POUR LA SEQ ID NO: 3: (i) CARACTERISTIQUES DE LA SEQUENCE: (A) LONGUEUR: 694 nucléotides (B) TYPE: nucléotide (C) NOMBRE DE BRINS: simple (D) CONFIGURATION: circulaire (ii) TYPE DE MOLECULE: ADN (ix) CARACTERISTIQUE: (A) NOM/CLE: pCRE-LBD[GR(I747/T)] plasmide d'expression de la protéine de fusion CRE-LBD[GR(I747/T)] (B) EMPLACEMENT:1..6091 (xi) DESCRIPTION DE LA SEQUENCE: SEQ ID NO: 1: GTCGACTTCT GAGGCGGAAA GAACCAGCTG TGGAATGTGT GTCAGTTAGG GTGTGGAAAG 6 TCCCCAGGCT CCCCAGCAGG CAGAAGTATG CAAAGCATGC ATCTCAATTA GTCAGCAACC 120 AGCTGTGGAA AGTCCCCAGG CTCCCCAGCA GGCGAAGTA TGCAAAGCAT CCATCTCAAT 18 TAGTCAGCAA CCATAGTCCC GCCCCTAACT CCGCCCATCC CGCCCCTAAC TCCGCCCAGT 24 TCCGCCCATT CTCCGCCCCA TGGCTGACTA ATTTTTTTTA TTTATGCAGA GGCCGAGGCC 300 GCCTCGGCCT CTGAGCTATT CCAGAAGTAG TGAGGAGGCT TTTTTGGAGG CCTAGGCTTT 360 TGCAAAAAGC TGGATCGATC CTGAGAACTT CAGGGTGAGT TTGGGGACCC TTGATTGTTC 42 TTTCTTTTTC GCTATTGTAA AATTCATGTT ATATGGAGGG GGCAAAGTTT TCAGGGTGTT 480 GTTTAGAATG GGAAGATGTC CCTTGTATCA CCATGGACCC TCATGATAAT TTTGTTTCTT 540 TCACTTTCTA CTCTGTTGAC AACCATTGTC TCCTCTTATT TTCTTTTCAT TTTCTGTAAC 600 TTTTTCGTTA AACTTTAGCT TGCATTTGTA ACGAATTTTT AAATTCACTT TTGTTTATTT 660 GTCAGATTGT AAGTACTTTC TCTAATCACT TTTTTTTCAA GGCAATCAGG GTATATTATA 720 TTGTACTTCA GCACAGTTTT AGAGAACAAT TGTTATAATT AAATGATAAG GTAGAATATT 780 TCTGCATATA AATTCTGGCT GGCGTGGAAA TATTCTTATT GGTAGAAACA ACTACATCCT 840 GGTCATCATC CTGCCTTTCT CTTTATGGTT ACAATGATAT ACACTGTTTG AGATGAGGAT 900 AAAATACTCT GAGTCCAAAC CGGGCCCCTC TGCTAACCAT GTTCATGCCT TCTTCTTTTT 960 CCTACAGCTC CTGGGCAACG TGCTGGTTAT TGTGCTGTCT CATCATTTTG GCAAAGAATT 1020 GTAATACGAC TCACTATAGG GCGAATTCCA CCATGTCCAA TTTACTGACC GTACACCAAA 1080 ATTTGCCTGC ATTACCGGTC GATGCAACGA GTGATGAGGT TCGCAAGAAC CTGATGGACA 1140 TGTTCAGGGA TCGCCAGGCG TTTTCTGAGC ATACCTGGAA AATGCTTCTG TCCGTTTGCC 1200 GGTCGTGGGC GGCATGGTGC AAGTTGAATA ACCGGAAATG GTTTCCCGCA GAACCTGAAG 1260 ATGTTCGCGA TTATCTTCTA TATCTTCAGG CGCGCGGTCT GGCAGTAAAA ACTATCCAGC 1320 AACATTTGGG CCAGCTAAAC ATGCTTCATC GTCGGTCCGG GCTGCCACGA CCAAGTGACA 1380 GCAATGCTGT TTCACTGGTT ATGCGGCGGA TCCGAAAAGA AAACGTTGAT GCCGGTGAAC 1440 GTGCAAAACA GGCTCTAGCG TTCGAACGCA CTGATTTCGA CCAGGTTCGT TCACTCATGG 1500 AAAATAGCGA TCGCTGCCAG GATATACGTA ATCTGGCATT TCTGGGGATT GCTTATAACA 1560 CCCTGTTACG TATAGCCGAA ATTGCCAGGA TCAGGGTTAA AGATATCTCA CGTACTGACG 1620 GTGGGAGAAT GTTAATCCAT ATTGGCAGAA CGAAAACGCT GGTTAGCACC GCAGGTGTAG 1680 AGAAGGCACT TAGCCTGGGG GTAACTAAAC TGGTCGAGCG ATGGATTTCC GTCTCTGGTG 1740 TAGCTGATGA TCCGAATAAC TACCTGTTTT GCCGGGTCAG AAAAAATGGT GTTGCCGCGC 1800 CATCTGCCAC CAGCCAGCTA TCAACTCGCG CCCTGGAAGG GATTTTTGAA GCAACTCATC 1860 GATTGATTTA CGGCGCTAAG GATGACTCTG GTCAGAGATA CCTGGCCTGG TCTGGACACA 1920 GTGCCCGTGT CGGAGCCGCG CGAGATATGG CCCGCGCTGG AGTTTCAATA CCGGAGATCA 1980 TGCAAGCTGG TGGCTGGACC AATGTAAATA TTGTCATGAA CTATATCCGT AACCTGGATA 2040 GTGAAACAGG GGCAATGGTG CGCCTGCTGG AAGATGGCGA TCTCGAGATT CAGCAGGCCA 2100 CTACAGGAGT CTCACAAGAA ACCTCTGAAA ATCCTGGTAA CAAAACAATA GTTCCTGCAA 2160 CGTTACCACA ACTCACCCCT ACCCTGGTGT CACTGTTGGA GGTTATTGAA CCTGAAGTGT 2220 TATATGCAGG ATATGATAGC TCTGTTCCAG ACTCAACTTG GAGGATCATG ACTACGCTCA 2280 ACATGTTAGG AGGGCGGCAA GTGATTGCAG CAGTGAAATG GGCAAAGGCA ATACCAGGTT 2340 TCAGGAACTT ACACCTGGAT GACCAAATGA CCCTACTGCA GTACTCCTGG ATGTTTCTTA 2400 TGGCATTTGC TCTGGGGTGG AGATCATATA GACAATCAAG TGCAAACCTG CTGTGTTTTG 2460 CTCCTGATCT GATTATTAAT GAGCAGAGAA TGACTCTACC CTGCATGTAC GACCAATGTA 2520 AACACATGCT GTATGTTTCC TCTGAGTTAC ACAGGCTTCA GGTATCTTAT GAAGAGTATC 2580 TCTGTATGAA AACCTTACTG CTTCTCTCTT CAGTTCCTAA GGACGGTCTG AAGAGCCAAG 2640 AGCTATTTGA TGAAATTAGA ATGACCTACA TCAAAGAGCT AGGAAAAGCC ATTGTCAAGA 2700 GGGAAGGAAA CTCCAGCCAG AACTGGCAGC GGTTTTATCA ACTGACAAAA CTCTTGGATT 2760 CTATGCATGA AGTGGTTGAA AATCTCCTTA ACTATTGCTT CCAAACATTT TTGGATAAGA 2820 CCATGTCCAC CGAGTTCCCC GAGATGTTAG CTGAAATCAT CACCAATCAG ATACCAAAAT 2880 ATTCAAATGG AAATATCAAA AAACTTCTGT TTCATCAAAA GGGTACCAGC CGTGGAGGGG 2940 CATCCGTGGA GGAGACGGAC CAAAGCCACT TGGCCACTGC GGGCTCTACT TCATCGCATT 3000 CCTTGCAAAA GTATTACATC ACGGGGGAGG CAGAGGGTTT CCCTGCCACA GTCTGAGAGC 3060 TCCCTGGAAT TCGGATCTTA TTAAAGCAGA ACTTGTTTAT TGCAGCTTAT AATGGTTACA 3120 AATAAAGCAA TAGCATCACA AATTTCACAA ATAAAGCATT TTTTTCACTG CATTCTAGTT 3180 GTGGTTTGTC CAAACTCATC AATGTATCTT ATCATGTCTG GTCGACTCTA GACTCTTCCG 3240 CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG GTATCAGCTC 3300 ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT 3360 GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC 3420 ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA 3480 ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC 3540 CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG 3600 CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC 3660 TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC 3720 GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA 3780 GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT 3840 ACGGCTACAC TAGAAGGACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG 3900 GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT 396# TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT 4#2# TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA 4#8# GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA 414# TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA ATGCTTAATC AGTGAGGCAC 42## CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC CTGACTCCCC GTCGTGTAGA 426# TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC TGCAATGATA CCGCGAGACC 432# CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC AGCCGGAAGG GCCGAGCGCA 438# GAAGTGGTCC TGCAACTTTA TCCGCCTCCA TCCAGTCTAT TAATTGTTGC CGGGAAGCTA 444# GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT TGCCATTGCT ACAGGCATCG 45## TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC CGGTTCCCAA CGATCAAGGC 456# GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT CCTCCGATCG 462# TTGTCAGAAG TAACTTGGCC GCAGTGTTAT CACTCATGGT TATGGCAGCA CTGCATAATT 468# CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC TGGTGAGTAC TCAACCAAGT 474# CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG CCCGGCGTCA ATACGGGATA 48## ATACCGCGCC ACATAGCAGA ACTTTAAAAG TGCTCATCAT TGGAAAACGT TCTTCGGGGC 486# GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC GATGTAACCC ACTCGTGCAC 492# CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC TGGGTGAGCA AAAACAGGAA 498# GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA ATGTTGAATA CTCATACTCT 5#4# TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT 51## TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC 516# CACCTGACGT CTAAGAAACC ATTATTATCA TGACATTAAC CTATAAAAAT AGGCGTATCA 522# CGAGGCCCCT TTCGTCTCGC GCGTTTCGGT GATGACGGTG AAAACCTCTG ACACATGCAG 528# CTCCCGGAGA CGGTCACAGC TTGTCTGTAA GCGGATGCCG GGAGCAGACA AGCCCGTCAG 534# GGCGCGTCAG CGGGTGTTGG CGGGTGTCGG GGCTGGCTTA ACTATGCGGC ATCAGAGCAG 54## ATTGTACTGA GAGTGCACCA TATGCGGTGT GAAATACCGC ACAGATGGGT AAGGAGAAAA 546# TACCGCATCA GGAAATTGTA AACGTTAATA TTTTGTTAAA ATTCGCGTTA AATTTTTGTT 552# AAATCAGCTC ATTTTTTAAC CAATAGGCCG AAATCGGCAA AATCCCTTAT AAATCAAAAG 558# AATAGACCGA GATAGGGTTG AGTGTTGTTC CAGTTTGGAA CAAGAGTCCA CTATTAAAGA 564# ACGTGGACTC CAACGTCAAA GGGCGAAAAA CCGTCTATCA GGGCGATGGC CCACTACGTG 57## AACCATCACC CTAATCAAGT TTTTTGGGGT CGAGGTGCCG TAAAGCACTA AATCGGAACC 576# CTAAAGGGAG CCCCCGATTT AGAGCTTGAC GGGGAAAGCC GGCGAACGTG GCGAGAAAGG 582# AAGGGAAGAA AGCGAAAGGA GCGGGCGCTA GGGCGCTGGC AAGTGTAGCG GTCACGCTGC 588# GCGTAACCAC CACACCCGCC GCGCTTAATG CGCCGCTACA GGGCGCGTCC CGCCATTCGC 504# CATTCAGGCT GCGCAACTGT TGGGAAGGGC GATCGGTGCG GGCCTCTTCG CTATTACGCC 6### AGCTGGCGAA AGGGGGATGT GCTGCAAGGC GATTAAGTTG GGTAACGCCA GGGTTTTCCC 6#6# AGTCACGACG TTGTAAAACG ACGGCCAGTG AATT 6#94