LISTE DE SEQUENCES (1) INFORMATIONS GENERALES: (i) DEPOSANT: (A) NOM: INSTITUT PASTEUR (B) RUE: 28 Rue du Docteur Roux (C) VILLE: PARIS (E) PAYS: FRANCE (F) CODE POSTAL: 75724 CEDEX 15 (ii) TITRE DE L' INVENTION: "POLYPEPTIDE COMPORTANT UN DOMAINE COHESINE, COMPOSITION ENZYMATIQUE EN COMPORTANT ET FRAGMENTS D'ADN CODANT POUR CES POLYPEPTIDES" (iii) NOMBRE DE SEQUENCES: 4 (iv) FORME DECHIFFRABLE PAR ORDINATEUR: (A) TYPE DE SUPPORT: Floppy disk (B) ORDINATEUR: IBM PC compatible (C) SYSTEME D' EXPLOITATION: PC-DOS/MS-DOS (D) LOGICIEL: PatentIn Release #1.0, Version #1.3 (OEB) (2) INFORMATIONS POUR LA SEQ ID NO: 1: (t) CARACTERISTIQUES DE LA SEQUENCE: (A) LONGUEUR: 1893 paires de bases (B) TYPE: nucléotide (C) NOMBRE DE BRINS: simple (ii) TYPE DE MOLECULE: ADN (ix) CARACTERISTIQUE: (A) NOM/CLE: SdbA de Clostridium thermocellum (B) EMPLACEMENT:1..1893 (xi) DESCRIPTION DE LA SEQUENCE: SEQ ID NO: 1: ATG AGG AAG AAA AAA AGA TTA ATA TCA TTA CTG CTT GCG GTT TH ATC 48 Met Arg Lys Lys Lys Arg Leu île Ser Leu Leu Leu Ala Val Phe Ile 1 5 10 15 GCC GTT GCA TGT CTG CCG GCG GGA ATT GCA AGG GCA GAT AAA GCC TCG 96 Ala Val Ala Cys Leu Pro Ala Gly Ile Ala Arg Ala Asp Lys Ala Ser 20 25 AGC ATT GAG CTT AAG TTT GAC CGC AAT AAG GGA GAA GTT GGA GAT ATA 144 Ser Ile Glu Leu Lys Phe Asp Arg Asn Lys Gly Glu Val Gly Asp Ile 35 4 45 CTT ATT GGT ACC GTA AGG ATA AAC AAT ATC AAG AAT TTC GCA GGA TTT 192 Leu île Gly Thr Val Arg Ile Asn Asn Ile Lys Asn Phe Ala Gly Phe se 55 6 CAG GTA AAC ATT GTA TAT GAT CCA AAA GTC TTA ATG GCT GTT GAC CCT 24 Gln Val Asn Ile Val Tyr Asp Pro Lys Val Leu Met Ala Val Asp Pro 65 7 75 8 GAA ACG GGG AAA GAA TTT ACT TCT TCA ACA TTT CCG CCA GGA CGC ACT 288 Glu Thr Gly Lys Glu Phe Thr Ser Ser Thr Phe Pro Pro Gly Arg Thr 85 9 95 GTA CTG AAA AAC AAT GCT TAC GGC CCA ATA CAG ATT GCG GAC AAT GAT 336 Val Leu Lys Asn Asn Ala Tyr Gly Pro Ile Gln Ile Ala Asp Asn Asp 100 105 110 CCG GAA AAA GGG ATA CTG AAC TTC GCG CTT GCA TAT TCA TAT ATT GCG 384 Pro Glu Lys Gly Ile Leu Asn Phe Ala Leu Ala Tyr Ser Tyr Ile Ala 115 120 125 GGA TAC AAA GAA ACA GGA GTA GCG GAG GAA AGC GGC ATA AH GCG AAA 432 Gly Tyr Lys Glu Thr Gly Val Ala Glu Glu Ser Gly Ile Ile Ala Lys 130 135 140 ATT GGA TTT AAA ATA CTC CAG AAA AAG AGC ACT GCC GTA AAA TTC CAG 480 Ile Gly Phe Lys Ile Leu Gîn Lys Lys Ser Thr Ala Val Lys Phe Gîn 145 150 155 160 GAT ACA TTA AGC ATG CCC GGA GCT ATT TCG GGA ACA CAG CTG TTT GAC 528 Asp Thr Leu Ser Met Pro Gly Ala Ile Ser Gly Thr Gln Leu Phe Asp 165 170 175 TGG GAC GGA GAA GTT ATT ACC GGA TAT GAG GTA ATA CAG CCG GAT GTG 576 Trp Asp Gly Glu Val Ile Thr Gly Tyr Glu Val Ile Gln Pro Asp Val 180 185 190 CTG AGT TTG GGT GAC GAG CCT TAT GAG ACA CCG GGA ACG GAT ATT CCG 624 Leu Ser Leu Gly Asp Glu Pro Tyr Glu Thr Pro Gly Thr Asp Ile Pro 195 200 205 ATA TCC GAC AAT CCG GCA GCA ACT CCG TCA TCC ACG CCG TCA GTT ACT 672 Ile Ser Asp Asn Pro Ala Ala Thr Pro Ser Ser Thr Pro Ser Val Thr 210 215 220 CCT TCA CCG GAA GTT AAA CCG ACT CAG ACG CCT TCG CCT GCA GAA AAT 720 Pro Ser Pro Glu Val Lys Pro Thr Gîn Thr Pro Ser Pro Ala Glu Asn 225 230 235 240 TCT GCA AAA GTG GAG CTT GAA CCT GTG TTG GAT AAT GCA ACA GGA GAA 768 Ser Ala Lys Val Glu Leu Glu Pro Val Leu Asp Asn Ala Thr Gly Glu 245 250 255 GCA AAG GCG GCA ATA GAT GAA GAA AAA TTA AAC AAG GCT CTT GAT GAA 816 Ala Lys Ala Ala île Asp Glu Glu Lys Leu Asn Lys Ala Leu Asp Glu 260 265 270 GCG AAA AAA TCG GAA GAT GAC AAA CTT GTG GAA CTT AAC ATA AAG AAG 864 Ala Lys Lys Ser Glu Asp Asp Lys Leu Val Glu Leu Asn Ile Lys Lys 275 280 285 GTT GAA AAT GCC GAT GCT TAC ATA CAA CAG CTT CCG GCG AAA TTC CTG 912 Val Glu Asn Ala Asp Ala Tyr île Gîn Gln Leu Pro Ala Lys Phe Leu 290 295 300 ATA AAA AGT GAC GCC GAA TAT AAG CTG AGA ATA GCT ACA GAG CAG GGA 960 Ile Lys Ser Asp Ala Glu Tyr Lys Leu Arg Ile Ala Thr Glu Gîn Gly 305 310 315 320 AH ATA GAA GTA CCG GCC AAC ATG CTG AAT ACT GCG GAT ATT TCA AAG 1008 Ile Ile Glu Val Pro Ala Asn Met Leu Asn Thr Ala Asp Ile Ser Lys 325 330 335 CTT GTA AAA AAT GAC TCC GTT GTT GAA TTC GTC ATA AGA AAA GTA AAA 1056 Leu Val Lys Asn Asp Ser Val Val Glu Phe Val Ile Arg Lys Val Lys 340 345 350 GTC GAT GAA CTT GGT GCA GAG CTC AAA GAG AAG ATA GGC AAC AGG CCG 1104 Val Asp Glu Leu Gly Ala Glu Leu Lys Glu Lys Ile Gly Asn Arg Pro 355 360 365 GTG ATT GAC ATA AGC GTG GH GTT GAC GGC AAA AAA GTT GAA TGG AGC 1152 Val Ile Asp île Ser Val Val Val Asp Gly Lys Lys Val Glu Trp Ser 370 375 380 AAT TAC AAA GCC AAG GTT AAA ATA TCA ATT CCT TAC AAG CCT GAT GCA 1200 Asn Tyr Lys Ala Lys Val Lys Ile Ser île Pro Tyr Lys Pro Asp Ala 385 390 395 400 AAA GAG CTG GAG AAC CAC GAG CAT ATT GTT GTA CTC CAT ATT GAT GAC 1248 Lys Glu Leu Glu Asn His Glu His Ile Val Val Leu His Ile Asp Asp 405 410 415 GCC GGC AAG GCA GH TCC GTA CCC AGC GGA AAA TAT GAA CCT TCT TTG 1296 Ala Gly Lys Ala Val Ser Val Pro Ser Gly Lys Tyr Glu Pro Ser Leu 420 425 430 GGC GTC GTT ACG TTT GAG ACG AAT CAT TTA AGC AAG TAT GCG GTT TCA 1344 Gly Val Val Thr Phe Glu Thr Asn His Leu Ser Lys Tyr Ala Val Ser 435 440 445 TAT GTT TAC AAG ACT TTC GCG GAT ATT GGT TCA TAT GCC TGG GCT AAA 1392 Tyr Val Tyr Lys Thr Phe Ala Asp Ile Gly Ser Tyr Ala Trp Ala Lys 450 455 460 AAG CAG ATA GAG GTT TTG GCT TCC AAA GGA GTA ATT AAC GGT ACA TCC 1440 Lys Gln île Glu Val Leu Ala Ser Lys Gly Val île Asn Gly Thr Ser 465 470 475 480 GAT ACC ACT HT ACG CCC CAG GCA GAC ATA ACA AGG GCG GAT TTC ATG 1488 Asp Thr Thr Phe Thr Pro Gin Ala Asp Ile Thr Arg Ala Asp Phe Met 485 490 495 ATA CTT CTT GTA AAG GCA CTG GGA TTG ACT GCC GAG GTT ACT TCC AAT 1536 île Leu Leu Val Lys Ala Leu Gly Leu Thr Ala Glu Val Thr Ser Asn 500 505 510 TTT GAT GAT GTG TCC GAA AAA GAC TAC TAT TAT GAA TAC GTG GGA ATT 1584 Phe Asp Asp Val Ser Glu Lys Asp Tyr Tyr Tyr Glu Tyr Val Gly île 515 520 525 GCA AAA GAG CTT GGA ATT ACG ACA GGA GTC GGA AAC AAC AAG TTC AAT 1632 Ala Lys Glu Leu Gly île Thr Thr Gly Val Gly Asn Asn Lys Phe Asn 530 535 540 CCG AAA GCC AAA ATT ACA AGA CAG GAT ATG ATG GTA CTT ACA ACA AAT 1680 Pro Lys Ala Lys lie Thr Arg Gln Asp Met Met Val Leu Thr Thr Asn 545 550 555 560 GCT CTC AGG ATT GCA GGA AAA ATA TCG AGC ACA GGA ACC CGC GCT GAT 1728 Ala Leu Arg lie Ala Gly Lys île Ser Ser Thr Gly Thr Arg Ala Asp 565 570 575 GTT GAA AGA TTT TCG GAC AAG GAC CAG ATA GCT TCA TAT GCG GTT GAA 1776 Val Glu Arg Phe Ser Asp Lys Asp Gln Ile Ala Ser Tyr Ala Val Glu 580 585 590 GGC GTT GCA ACC TTG GTA AAA GAA GGT ATT GTA GTG GGA AGC GGC GAT 1824 Gly Val Ala Thr Leu Val Lys Glu Gly Ile Val Val Gly Ser Gly Asp 595 600 605 ATT ATA AAT CCA AGG GGA AAT GCT TCA AGA GCC GAA CTT GCA GCA ATC 1872 île île Asn Pro Arg Gly Asn Ala Ser Arg Ala Glu Leu Ala Ala Ile 610 615 620 ATA TAC AAG ATT TAC TAC AAG 1893 Ile Tyr Lys Ile Tyr Tyr Lys 625 630 (3) INFORMATIONS POUR LA SEQ ID NO: 2: (i) CARACTERISTIQUES DE LA SEQUENCE: (A) LONGUEUR: 4992 paires de bases (B) TYPE: nucléotide (C) NOMBRE DE BRINS: simple (ii) TYPE DE MOLECULE: ADN (ix) CARACTERISTIQUE: (A) NOM/CLE: OlpB de Clostridium thermocellum (B) EMPLACEMENT:1..4992 (xi) DESCRIPTION DE LA SEQUENCE: SEQ ID NO: 2: ATG AAA CGA AAA AAT AAA GTA TTA TCA ATT TTG TTA ACT CTG CTG CTA 48 Met Lys Arg Lys Asn Lys Val Leu Ser île Leu Leu Thr Leu Leu Leu 1 5 10 15 ATA ATC TCT ACC ACA TCC GTA AAC ATG TCT TTT GCT GAA GCA ACT CCA 96 Ile île Ser Thr Thr Ser Val Asn Met Ser Phe Ala Glu Ala Thr Pro 20 25 30 AGT ATT GAA ATG GTT CTT GAT AAA ACT GAA GTC CAT GTA GGA GAT GTA 144 Ser Ile Glu Met Val Leu Asp Lys Thr Glu Val His Val Gly Asp Val 35 40 45 ATA ACG GCC ACA ATA AAA GTC AAT AAC ATT AGA AAA TTG GCG GGA TAT 192 Ile Thr Ala Thr Ile Lys Val Asn Asn Ile Arg Lys Leu Ala Gly Tyr 50 55 60 CAG CTA AAT ATC AAA TTT GAC CCT GAA GTT TTA CAG CCG GTA GAC CCT 240 Gîn Leu Asn Ile Lys Phe Asp Pro Glu Val Leu Gln Pro Val Asp Pro 65 70 75 80 GCA ACA GGA GAG GAA TTT ACT GAT AAG TCC ATG CCG GTA AAT AGG GTT 288 Ala Thr Gly Glu Glu Phe Thr Asp Lys Ser Met Pro Val Asn Arg Val 85 90 95 TTG CTG ACA AAC AGC AAA TAT GGA CCT ACT CCT GTG GCG GGT AAC GAT 336 Leu Leu Thr Asn Ser Lys Tyr Gly Pro Thr Pro Val Ala Gly Asn Asp 100 105 110 ATA AAG TCA GGA AH ATT AAT TTT GCT ACG GGA TAT AAC AAT TTA ACA 384 Ile Lys Ser Gly île île Asn Phe Ala Thr Gly Tyr Asn Asn Leu Thr 115 120 125 GCG TAC AAA TCC AGC GGA ATA GAC GAA CAT ACA GGA ATA ATA GGA GAG 432 Ala Tyr Lys Ser Ser Gly Ile Asp Glu His Thr Gly Ile île Gly Glu 130 135 140 ATT GGT TTT AAA GTT TTA AAG AAA CAA AAT ACG TCT ATT AGG TTT GAA 480 Ile Gly Phe Lys Val Leu Lys Lys Gîn Asn Thr Ser Ile Arg Phe Glu 145 150 155 160 GAT ACA TTA TCG ATG CCC GGG GCA ATA TCG GGA ACA AGT TTG TTT GAC 528 Asp Thr Leu Ser Met Pro Gly Ala île Ser Gly Thr Ser Leu Phe Asp 165 170 175 TGG GAT GCA GAA ACT ATA ACA GGA TAT GAG GTA ATA CAG CCG GAT CTT 576 Trp Asp Ala Glu Thr île Thr Gly Tyr Glu Val île Gln Pro Asp Leu 180 185 190 ATA GTT GTA GAG GCA GAA CCG TTA AAA GAC GCC AGC GTG GCT CTG GAA 624 Ile Val Val Glu Ala Glu Pro Leu Lys Asp Ala Ser Val Ala Leu Glu 195 200 205 CTG GAT AAG ACG AAG GTA AAA GTA GGG GAC ATA ATA ACA GCG ACG ATA 672 Leu Asp Lys Thr Lys Val Lys Val Gly Asp île Ile Thr Ala Thr île 210 215 220 AAG ATA GAG AAC ATG AAG AAT TTT GCA GGG TAC CAG TTG AAT ATC AAG 720 Lys île Glu Asn Met Lys Asn Phe Ala Gly Tyr Gln Leu Asn Ile Lys 225 230 235 240 TAT GAC CCG ACC ATG TTG GAG GCA ATA GAA CTG GAG ACA GGA AGT GCG 768 Tyr Asp Pro Thr Met Leu Glu Ala Ile Glu Leu Glu Thr Gly Ser Ala 245 250 255 ATA GCG AAG AGG ACA TGG CCG GTT ACA GGA GGT ACT GTT CTG CAA AGT 816 Ile Ala Lys Arg Thr Trp Pro Val Thr Gly Gly Thr Val Leu Gîn Ser 260 265 270 GAC AAT TAT GGA AAG ACG ACT GCG GTA GCG AAT GAT GTA GGA GCA GGT 864 Asp Asn Tyr Gly Lys Thr Thr Ala Val Ala Asn Asp Val Gly Ala Gly 275 280 285 ATA ATA AAC TTT GCT GAG GCA TAC TCG AAC CTT ACC AAA TAC AGA GAG 912 Ile Ile Asn Phe Ala Glu Ala Tyr Ser Asn Leu Thr Lys Tyr Arg Glu 290 295 300 ACA GGT GTG GCA GAG GAG ACA GGT ATA ATA GGA AAG ATA GGC TTC AGA 960 Thr Gly Val Ala Glu Glu Thr Gly Ile Ile Gly Lys Ile Gly Phe Arg 305 310 315 320 GTA CTG AAG GCA GGA AGT ACG GCT ATA AGA TTT GAG GAT ACG ACA GCG 1008 Val Leu Lys Ala Gly Ser Thr Ala Ile Arg Phe Glu Asp Thr Thr Ala 325 330 335 ATG CCG GGA GCA ATA GAA GGA ACA TAC ATG TTC GAC TGG TAT GGC GAG 156 Met Pro Gly Ala Ile Glu Gly Thr Tyr Met Phe Asp Trp Tyr Gly Glu 340 345 350 AAC ATC AAA GGG TAT AGC GTA GTA CAG CCT GGG GAA ATA GTG GCA GAA 114 Asn île Lys Gly Tyr Ser Val Val Gîn Pro Gly Glu Ile Val Ala Glu 355 360 365 GGA GAA GAG CCG GGT GAA GAG CCG ACA GAA GAG CCT GTA CCG ACA GAG 1152 Gly Glu Glu Pro Gly Glu Glu Pro Thr Glu Glu Pro Val Pro Thr Glu 370 375 380 ACA CCA GTA GAT CCC ACA CCG ACA GTG ACA GAA GAG CCT GTA CCT TCA îzee Thr Pro Val Asp Pro Thr Pro Thr Val Thr Glu Glu Pro Val Pro Ser 385 390 395 400 GAG CTT CCA GAT TCC TAT GTA ATA ATG GAA CTG GAT AAG ACG AAG GTA 1248 Glu Leu Pro Asp Ser Tyr Val île Met Glu Leu Asp Lys Thr Lys Val 405 410 415 AAA GTA GGG GAC ATA ATA ACA GCG ACG ATA AAG ATA GAG AAC ATG AAG 1296 Lys Val Gly Asp Ile île Thr Ala Thr Ile Lys île Glu Asn Met Lys 420 425 430 AAT HT GCA GGG TAC CAG TTG AAT ATC AAG TAT GAC CCG ACC ATG TTG 1344 Asn Phe Ala Gly Tyr Gln Leu Asn Ile Lys Tyr Asp Pro Thr Met Leu 435 440 445 GAG GCA ATA GAA CTG GAG ACA GGA AGT GCG ATA GCG AAG AGG ACA TGG 1392 Glu Ala île Glu Leu Glu Thr Gly Ser Ala Ile Ala Lys Arg Thr Trp 450 455 460 CCG GTT ACA GGA GGT ACT GTT CTG CAA AGT GAC AAT TAT GGA AAG ACG 1440 Pro Val Thr Gly Gly Thr Val Leu Gln Ser Asp Asn Tyr Gly Lys Thr 465 470 475 480 ACT GCG GTA GCG AAT GAT GTA GGA GCA GGT ATA ATA AAC TTT GCT GAG 1488 Thr Ala Val Ala Asn Asp Val Gly Ala Gly Ile île Asn Phe Ala Glu 485 490 495 GCA TAC TCG AAC CTT ACC AAA TAC AGA GAG ACA GGT GTG GCA GAG GAG 1536 Ala Tyr Ser Asn Leu Thr Lys Tyr Arg Glu Thr Gly Val Ala Glu Glu 500 505 510 ACA GGT ATA ATA GGA AAG ATA GGC TTC AGA GTA CTG AAG GCA GGA AGT 1584 Thr Gly île île Gly Lys île Gly Phe Arg Val Leu Lys Ala Gly Ser 515 520 525 ACG GCT ATA AGA TTT GAG GAT ACG ACA GCG ATG CCG GGA GCA ATA GAA 1632 Thr Ala Ile Arg Phe Glu Asp Thr Thr Ala Met Pro Gly Ala Ile Glu 530 535 540 GGA ACA TAC ATG TTC GAC TGG TAT GGC GAG AAC ATC AAA GGG TAT AGC 1680 Gly Thr Tyr ket Phe Asp Trp Tyr Gly Glu Asn Ile Lys Gly Tyr Ser 545 550 555 560 GTA GTA CAG CCT GGG GAA ATA GTG GCG GAA GGA GAA GAG CCG ACA GAA 1728 Val Val Gîn Pro Gly Glu Ile Val Ala Glu Gly Glu Glu Pro Thr Glu 565 570 575 GAG CCT GTA CCG ACA GAG ACA CCA GTA GAT CCC ACA CCG ACA GTG ACA 1776 Glu Pro Val Pro Thr Glu Thr Pro Val Asp Pro Thr Pro Thr Val Thr 580 585 590 GAA GAG CCT GTA CCT TCA GAG CTT CCA GAT TCC TAT GTG ATA ATG GAA 1824 Glu Glu Pro Val Pro Ser Glu Leu Pro Asp Ser Tyr Val Ile Met Glu 595 600 605 TTG GAT AAG ACG AAG GTA AAA GAA GGC GAC GTA ATA ATA GCA ACA ATA 1872 Leu Asp Lys Thr Lys Val Lys Glu Gly Asp Val Ile Ile Ala Thr Ile 610 615 620 AGA GTA AAT AAC ATA AAG AAT CTT GCC GGA TAT CAG ATA GGC ATC AAA 1920 Arg Val Asn Asn île Lys Asn Leu Ala Gly Tyr Gln île Gly île Lys 625 630 635 640 TAT GAC CCG AAA GTA TTA GAG GCA TTT AAT ATC GAG ACA GGG GAC CCA 1968 Tyr Asp Pro Lys Val Leu Glu Ala Phe Asn Ile Glu Thr Gly Asp Pro 645 650 655 ATA GAT GAA GGA ACA TGG CCT GCA GTA GGG GGA ACA ATA CTG AAG AAT 2016 Ile Asp Glu Gly Thr Trp Pro Ala Val Gly Gly Thr île Leu Lys Asn 660 665 670 AGA GAT TAC CTG CCG ACT GGG GTA GCA ATA AAC AAT GTA TCT AAA GGA 2064 Arg Asp Tyr Leu Pro Thr Gly Val Ala Ile Asn Asn Val Ser Lys Gly 675 680 685 ATA CTG AAT TTT GCT GCT TAT TAC GTT TAC TTC GAT GAC TAT AGA GAG 2112 île Leu Asn Phe Ala Ala Tyr Tyr Val Tyr Phe Asp Asp Tyr Arg Glu 690 695 700 GAA GGA AAG TCA GAA GAT ACA GGA ATT ATA GGA AAT ATA GGC TTT AGA 2160 Glu Gly Lys Ser Glu Asp Thr Gly Ile Ile Gly Asn Ile Gly Phe Arg 705 710 715 720 GTA CTG AAG GCG GAA GAT ACA ACG ATA AGA TTT GAA GAG CTG GAG TCA 2208 Val Leu Lys Ala Glu Asp Thr Thr île Arg Phe Glu Glu Leu Glu Ser 725 730 735 ATG CCG GGT TCA ATA GAC GGA ACA TAT ATG TTG GAT TGG TAT CTT AAT 2256 Met Pro Gly Ser Ile Asp Gly Thr Tyr Met Leu Asp Trp Tyr Leu Asn 740 745 750 AGA ATC TCT GGC TAT GTA GTA ATA CAA CCG GCG CCT ATA AAG GCG GCT 2304 Arg Ile Ser Gly Tyr Val Val île Gln Pro Ala Pro Ile Lys Ala Ala 755 760 765 AGT GAC GAA CCA ATA CCA ACG GAT ACA CCA TCA GAT GAA CCG ACA CCG 2352 Ser Asp Glu Pro île Pro Thr Asp Thr Pro Ser Asp Glu Pro Thr Pro 770 775 780 TCA GAC GAG CCA ACG CCA TCT GAC GAA CCG ACA CCG TCT GAT GAG CCA 24 Ser Asp Glu Pro Thr Pro Ser Asp Glu Pro Thr Pro Ser Asp Glu Pro 785 790 795 800 ACA CCG TCA GAT GAA CCG ACT CCG TCA GAG ACA CCT GAG GAG CCG ATA 2448 Thr Pro Ser Asp Glu Pro Thr Pro Ser Glu Thr Pro Glu Glu Pro île 805 810 815 CCG ACG GAT ACA CCA TCA GAT GAA CCG ACA CCA TCA GAC GAG CCA ACG 2496 Pro Thr Asp Thr Pro Ser Asp Glu Pro Thr Pro Ser Asp Glu Pro Thr 820 825 830 CCA TCT GAT GAA CCA ACA CCG TCT GAT GAG CCA ACA CCA TCT GAT GAA 2544 Pro Ser Asp Glu Pro Thr Pro Ser Asp Glu Pro Thr Pro Ser Asp Glu 835 840 845 CCG ACT CCG TCA GAG ACA CCT GAG GAG CCG ATA CCG ACG GAT ACA CCA 2592 Pro Thr Pro Ser Glu Thr Pro Glu Glu Pro île Pro Thr Asp Thr Pro 850 855 860 TCA GAT GAA CCG ACA CCG TCA GAC GAG CCA ACG CCA TCT GAC GAA CCA 264 Ser Asp Glu Pro Thr Pro Ser Asp Glu Pro Thr Pro Ser Asp Glu Pro 865 87 875 880 ACA CCG TCT GAT GAG CCA ACA CCG TCA GAT GAA CCG ACT CCG TCA GAG 2688 Thr Pro Ser Asp Glu Pro Thr Pro Ser Asp Glu Pro Thr Pro Ser Glu 885 890 895 ACA CCT GAG GAG CCG ATA CCG ACG GAT ACA CCA TCA GAT GAA CCG ACA 2736 Thr Pro Glu Glu Pro île Pro Thr Asp Thr Pro Ser Asp Glu Pro Thr 900 905 910 CCG TCA GAC GAG CCA ACG CCA TCT GAC GAA CCA ACA CCG TCT GAT GAG 2784 Pro Ser Asp Glu Pro Thr Pro Ser Asp Glu Pro Thr Pro Ser Asp Glu 915 920 925 CCA ACA CCG TCA GAT GAA CCG ACT CCG TCA GAG ACA CCT GAG GAG CCG 2832 Pro Thr Pro Ser Asp Glu Pro Thr Pro Ser Glu Thr Pro Glu Glu Pro 930 935 940 ATA CCG ACG GAT ACA CCA TCA GAT GAA CCG ACA CCG TCA GAC GAG CCG 2880 île Pro Thr Asp Thr Pro Ser Asp Glu Pro Thr Pro Ser Asp Glu Pro 945 950 955 960 ATA CCA TCT GAC GAA CCA ACA CCG TCA GAC GAG CCA ACG CCA TCT GAC 2928 Thr Pro Ser Asp Glu Pro Thr Pro Ser Asp Glu Pro Thr Pro Ser Asp 965 970 975 GAA CCG ACA CCG TCT GAT GAG CCA ACA CCA TCT GAT GAA CCG ACT CCG 2976 Glu Pro Thr Pro Ser Asp Glu Pro Thr Pro Ser Asp Glu Pro Thr Pro 980 985 990 TCA GAG ACA CCT GAG GAG CCG ATA CCG ACG GAT ACA CCA TCA GAT GAA 3024 Ser Glu Thr Pro Glu Glu Pro Ile Pro Thr Asp Thr Pro Ser Asp Glu 995 1000 1005 CCG ACA CCG TCA GAC GAG CCG ACA CCA TCT GAC GAA CCA ACA CCG TCA 3072 Pro Thr Pro Ser Asp Glu Pro Thr Pro Ser Asp Glu Pro Thr Pro Ser 1010 1015 1020 GAC GAG CCA ACG CCA TCT GAC GAA CCG ACA CCG TCT GAT GAG CCA ACA 3120 Asp Glu Pro Thr Pro Ser Asp Glu Pro Thr Pro Ser Asp Glu Pro Thr 1025 1030 1035 1040 CCA TCT GAT GAA CCG ACT CCG TCA GAG ACA CCT GAG GAG CCG ATA CCG 3168 Pro Ser Asp Glu Pro Thr Pro Ser Glu Thr Pro Glu Glu Pro île Pro 1045 1050 1055 ACG GAT ACA CCA TCA GAT GAA CCG ACA CCG TCA GAC GAG CCG ACA CCA 3216 Thr Asp Thr Pro Ser Asp Glu Pro Thr Pro Ser Asp Glu Pro Thr Pro 1060 1065 1070 TCT GAC GAA CCA ACA CCG TCT GAT GAG CCA ACA CCG TCA GAT GAA CCG 3264 Ser Asp Glu Pro Thr Pro Ser Asp Glu Pro Thr Pro Ser Asp Glu Pro 1075 1080 1085 ACT CCG TCA GAG ACA CCT GAG GAG CCG ATA CCG ACG GAT ACA CCA TCA 3312 Thr Pro Ser Glu Thr Pro Glu Glu Pro île Pro Thr Asp Thr Pro Ser 1090 1095 1100 GAT GAA CCG ACA CCG TCA GAC GAG CCA ACG CCA TCT GAC GAA CCG ACA 3360 Asp Glu Pro Thr Pro Ser Asp Glu Pro Thr Pro Ser Asp Glu Pro Thr 1105 1110 1115 1120 CCG TCT GAT GAG CCA ACA CCG TCA GAT GAA CCG ACT CCG TCA GAG ACA 3408 Pro Ser Asp Glu Pro Thr Pro Ser Asp Glu Pro Thr Pro Ser Glu Thr 1125 1130 1135 CCT GAG GAG CCG ATA CCG ACG GAT ACA CCA TCA GAT GAA CCC ACA CCC 3456 Pro Glu Glu Pro Ile Pro Thr Asp Thr Pro Ser Asp Glu Pro Thr Pro 1140 1145 1150 TCA GAC GAG CCA ACG CCA TCT GAC GAA CCG ACA CCG TCT GAT GAG CCA 3504 Ser Asp Glu Pro Thr Pro Ser Asp Glu Pro Thr Pro Ser Asp Glu Pro 1155 1160 1165 ACA CCG TCA GAT GAA CCG ACT CCG TCA GAG ACA CCT GAG GAG CCG ATA 3552 Thr Pro Ser Asp Glu Pro Thr Pro Ser Glu Thr Pro Glu Glu Pro lie 1170 1175 1180 CCG ACG GAT ACA CCA TCA GAT GAA CCG ACA CCA TCA GAC GAG CCA ACG 3600 Pro Thr Asp Thr Pro Ser Asp Glu Pro Thr Pro Ser Asp Glu Pro Thr 1185 1190 1195 1200 CCA TCT GAT GAA CCA ACA CCG TCT GAT GAG CCA ACA CCA TCT GAT GAA 3648 Pro Ser Asp Glu Pro Thr Pro Ser Asp Glu Pro Thr Pro Ser Asp Glu 1205 1210 1215 CCG ACT CCG TCA GAG ACA CCT GAG GAG CCG ATA CCG ACG GAT ACA CCA 3696 Pro Thr Pro Ser Glu Thr Pro Glu Glu Pro île Pro Thr Asp Thr Pro 122 1225 1230 TCA GAT GAA CCG ACA CCG TCA GAC GAG CCA ACG CCA TCT GAC GAA CCA 3744 Ser Asp Glu Pro Thr Pro Ser Asp Glu Pro Thr Pro Ser Asp Glu Pro 1235 1240 1245 ACA CCG TCT GAT GAG CCA ACA CCG TCA GAT GAA CCG ACT CCG TCA GAG 3792 Thr Pro Ser Asp Glu Pro Thr Pro Ser Asp Glu Pro Thr Pro Ser Glu 1250 1255 1260 ACA CCT GAG GAG CCG ATA CCG ACG GAT ACA CCA TCA GAT GAA CCG ACA 3840 Thr Pro Glu Glu Pro île Pro Thr Asp Thr Pro Ser Asp Glu Pro Thr 1265 1270 1275 1280 CCG TCA GAC GAG CCG ACA CCA TCT GAC GAA CCA ACA CCG TCA GAC GAG 3888 Pro Ser Asp Glu Pro Thr Pro Ser Asp Glu Pro Thr Pro Ser Asp Glu 1285 1290 1295 CCA ACG CCA TCT GAC GAA CCG ACA CCG TCT GAT GAG CCA ACA CCA TCT 3936 Pro Thr Pro Ser Asp Glu Pro Thr Pro Ser Asp Glu Pro Thr Pro Ser 1300 1305 1310 GAT GAA CCG ACT CCG TCA GAG ACA CCT GAG GAG CCG ATA CCG ACG GAT 3984 Asp Glu Pro Thr Pro Ser Glu Thr Pro Glu Glu Pro lie Pro Thr Asp 1315 1320 1325 ACA CCA TCA GAT GAA CCG ACA CCG TCA GAC GAG CCG ACA CCA TCT GAC 4032 Thr Pro Ser Asp Glu Pro Thr Pro Ser Asp Glu Pro Thr Pro Ser Asp 1330 1335 1340 GAA CCA ACA CCG TCA GAC GAG CCA ACG CCA TCT GAC GAA CCG ACA CCG 4080 Glu Pro Thr Pro Ser Asp Glu Pro Thr Pro Ser Asp Glu Pro Thr Pro 1345 1350 1355 1360 TCT GAT GAG CCA ACA CCA TCT GAT GAA CCG ACT CCG TCA GAG ACA CCT 4128 Ser Asp Glu Pro Thr Pro Ser Asp Glu Pro Thr Pro Ser Glu Thr Pro 1365 1370 1375 GAG GAG CCG ACA CCG ACT ACT ACA CCG ACA CCA ACA CCG TCG ACA ACG 4176 Glu Glu Pro Thr Pro Thr Thr Thr Pro Thr Pro Thr Pro Ser Thr Thr 1380 1385 1390 CCT ACA AGT GGC AGC GGA GGC AGT GGT GGA AGC GGT GGT GGC GGC GGA 4224 Pro Thr Ser Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Gly Gly Gly 1395 14e0 145 GGT GGT GGA GGA ACT GTA CCT ACA TCT CCA ACA CCG ACA CCG ACA TCT 4272 Gly Gly Gly Gly Thr Val Pro Thr Ser Pro Thr Pro Thr Pro Thr Ser 1410 1415 1420 AAA CCG ACG TCT ACA CCT GCA CCG ACA GAA ATC GAA GAG CCT ACA CCA 4320 Lys Pro Thr Ser Thr Pro Ala Pro Thr Glu île Glu Glu Pro Thr Pro 1425 1430 1435 1440 TCT GAT GTG CCT GGT GCA ATC GGT GGA GAA CAT AGA GCA TAC TTA AGA 4368 Ser Asp Val Pro Gly Ala Ile Gly Gly Glu His Arg Ala Tyr Leu Arg 1445 1450 1455 GGA TAT CCG GAT GGA AGC TTC AGG CCT GAA AGA AAT ATA ACA AGA GCT 4416 Gly Tyr Pro Asp Gly Ser Phe Arg Pro Glu Arg Asn Ile Thr Arg Ala 1460 1465 1470 GAA GCG GCG GTA ATC HT GCT AAG TTG CTT GGA GCC GAT GAA AGC TAT 4464 Glu Ala Ala Val lie Phe Ala Lys Leu Leu Gly Ala Asp Glu Ser Tyr 1475 1480 1485 GGA GCT CAG TCT GCA AGT CCA TAT AGT GAT TTG GCT GAT ACT CAC TGG 4512 Gly Ala Gin Ser Ala Ser Pro Tyr Ser Asp Leu Ala Asp Thr His Trp 1490 1495 1500 GCT GCA TGG GCA ATC AAA TTT GCA ACA AGC CAG GGC TTG TTC AAA GGA 4560 Ala Ala Trp Ala lie Lys Phe Ala Thr Ser Gln Gly Leu Phe Lys Gly 1505 1518 1515 1520 TAT CCG GAC GGT ACG HT AAA CCT GAT CAG AAC ATA ACG AGA GCG GAA 4608 Tyr Pro Asp Gly Thr Phe Lys Pro Asp Gin Asn île Thr Arg Ala Glu 1525 1530 1535 TTC GCA ACT GTG GTA CTC CAC TTC CTG ACA AAA GTT AAG GGT CAG GAA 4656 Phe Ala Thr Val Val Leu His Phe Leu Thr Lys Val Lys Gly Gln Glu 1540 1545 1550 ATA ATG AGC AAG CTT GCA ACA ATA GAT ATA AGT AAT CCG AAG HT GAC 4704 île Met Ser Lys Leu Ala Thr île Asp île Ser Asn Pro Lys Phe Asp 1555 1560 1565 GAT TGT GTC GGA CAT TGG GCA CAA GAG HT ATT GAG AAA TTG ACA AGC 4752 Asp Cys Val Gly His Trp Ala Gin Glu Phe Ile Glu Lys Leu Thr Ser 1570 1575 1580 TTG GGT TAT ATT AGT GGC TAT CCT GAC GGA ACG TTC AAG CCG CAA AAC 48 Leu Gly Tyr île Ser Gly Tyr Pro Asp Gly Thr Phe Lys Pro Gin Asn 1585 1590 1595 1600 TAT ATT AAA CGT TCC GAA AGT GTG GCA CTG ATT AAC AGA GCT CTG GAG 4848 Tyr île Lys Arg Ser Glu Ser Val Ala Leu île Asn Arg Ala Leu Glu 1605 1610 1615 AGA GGT CCG CTT AAT GGA GCG CCG AAG CTC TTC CCG GAT GTT AAC GAA 4896 Arg Gly Pro Leu Asn Gly Ala Pro Lys Leu Phe Pro Asp Val Asn Glu 1620 1625 1630 TCA TAC TGG GCA TTT GGC GAC ATT ATG GAC GGT GCT CTC GAC CAC AGT 4944 Ser Tyr Trp Ala Phe Gly Asp île Met Asp Gly Ala Leu Asp His Ser 1635 1640 1645 TAC ATT ATC GAA GAT GAG AAA GAA AAA TTC GTT AAA TTG CTC GAA GAT 4992 Tyr Ile lie Glu Asp Glu Lys Glu Lys Phe Val Lys Leu Leu Glu Asp 1650 1655 1660 (4) INFORMATIONS POUR LA SEQ ID NO: 3: (i) CARACTERISTIQUES DE LA SEQUENCE: (A) LONGUEUR: 2064 paires de bases (B) TYPE: nucléotide (C) NOMBRE DE BRINS: simple (ii) TYPE DE MOLECULE: ADN (ix) CARACTERISTIQUE: (A) NOM/CLE: ORF2p de Clostridium thermocellum (B) EMPLACEMENT:1..2064 (xi) DESCRIPTION DE LA SEQUENCE: SEQ ID NO: 3: ATG AAA AAA AAC AAT GTA TTA ACA ATA GCA GCT ATG ATA GCG CTT CTT 48 Met Lys Lys Asn Asn Val Leu Thr île Ala Ala Met île Ala Leu Leu 1 5 10 15 CTA ACC AGC TTA CTT ACA AGT ATA ACT TTT GGG GAG ACT TCG AGT ATA 96 Leu Thr Ser Leu Leu Thr Ser île Thr Phe Gly Glu Thr Ser Ser île 20 25 30 CCT TCA AGA ATA TCT ATG GAG CTT GAC AAG ACA AAA GCA AAC ATA GGC 144 Pro Ser Arg île Ser Met Glu Leu Asp Lys Thr Lys Ala Asn île Gly 35 40 45 GAC ATA ATT ATA GCC ACA ATA AGA ATT GAC AAT ATC AAT AAC TTT AGC 192 Asp île Ile île Ala Thr Ile Arg Ile Asp Asn lie Asn Asn Phe Ser 50 55 60 GGA TAT CAA HA AAT ATA AAG TAT GAT CCG TCA TAC CTC CAG GCA GTT 240 Gly Tyr Gln Leu Asn île Lys Tyr Asp Pro Ser Tyr Leu Gin Ala Val 65 70 75 80 AAT CCT TTG ACA GGA GAA CCG ATA AAA AAG AGA ACA ATG CCG GCA GTG 288 Asn Pro Leu Thr Gly Glu Pro lie Lys Lys Arg Thr Met Pro Ala Val 85 90 95 AAC GGC ACG GTG TTG TTA AAG GGA GAT CAG TAC AGT ATT ACT GAG GTT 336 Asn Gly Thr Val Leu Leu Lys Gly Asp Gln Tyr Ser Ile Thr Glu Val 100 105 110 GTA GAA AAT AAC GTC GAT GAA GGG ATT TTA AAT TTT GGC AAG GGA TAT 384 Val Glu Asn Asn Val Asp Glu Gly Ile Leu Asn Phe Gly Lys Gly Tyr 115 120 125 GCA AAT HA ACT GAA TAC AGG AAA AGC GGA AAA CCT GAA ACA ACC GGA 432 Ala Asn Leu Thr Glu Tyr Arg Lys Ser Gly Lys Pro Glu Thr Thr Gly 130 135 140 ATT ATT GGC AAG ATA GGA TTT AAA GCC TTA AAG CTT GGC AAG ACG GAG 480 Ile Ile Gly Lys Ile Gly Phe Lys Ala Leu Lys Leu Gly Lys Thr Glu 145 150 155 160 ATC AAA TTT GAG AAC ACA CCC GTC ATG CCT GGG GCA AAA GAA GGA ACA 528 Ile Lys Phe Glu Asn Thr Pro Val Met Pro Gly Ala Lys Glu Gly Thr 165 170 175 CTG CTG HT GAC TGG GAT GCA GAA ACT ATA ACG GAA TAT AAT GTA ATT 576 Leu Leu Phe Asp Trp Asp Ala Glu Thr Ile Thr Glu Tyr Asn Val île 180 185 190 CAG CCT AAA GAA CTT GCA ATA ACG TTA CCG GAC GAT GCA CAC ATT GCT 624 Gin Pro Lys Glu Leu Ala Ile Thr Leu Pro Asp Asp Ala His Ile Ala 195 200 205 TTG GAA CTT GAC AAG ACA AAA GTG AAA GTG GGA GAT GTA ATT GTT GCG 672 Leu Glu Leu Asp Lys Thr Lys Val Lys Val Gly Asp Val Ile Val Ala 210 215 220 ACA GTA AAA GCA AAG AAT ATG ACT AGT ATG GCG GGA ATT CAG GTA AAT 720 Thr Val Lys Ala Lys Asn Met Thr Ser Met Ala Gly Ile Gln Val Asn 225 230 235 240 ATT AAA TAT GAC CCT GAA GTA TTG CAG GCG ATT GAT CCT GCG ACG GGA 768 île Lys Tyr Asp Pro Glu Val Leu Gln Ala Ile Asp Pro Ala Thr Gly 245 250 255 AAA CCG HT ACA AAA GAA ACA HA CTT GTG GAC CCG GAA CTG TTA TCA 816 Lys Pro Phe Thr Lys Glu Thr Leu Leu Val Asp Pro Glu Leu Leu Ser 260 265 270 AAC AGA GAA TAT AAT CCG TTG TTA ACA GCA GTT AAT GAC ATA AAT TCC 864 Asn Arg Glu Tyr Asn Pro Leu Leu Thr Ala Val Asn Asp île Asn Ser 275 280 285 GGC ATT ATA AAT TAT GCA TCT TGT TAT GTA TAT TGG GAT TCC TAC AGA 912 Gly Ile île Asn Tyr Ala Ser Cys Tyr Val Tyr Trp Asp Ser Tyr Arg 290 295 300 GAA TCA GGA GTA TCT GAA AGC ACC GGA ATA ATT GGA AAG GTT GGC HT 960 Glu Ser Gly Val Ser Glu Ser Thr Gly Ile Ile Gly Lys Val Gly Phe 305 310 315 320 AAA GTG CTG AAA GCT GCC AAC ACC ACA GTA AAA CTG GAA GAA ACA AGA 1008 Lys Val Leu Lys Ala Ala Asn Thr Thr Val Lys Leu Glu Glu Thr Arg 325 330 335 HT ACA CCA AAT TCG ATA GAC GGT ACT TTG GTA ATT GAT TGG TAT GGC 1056 Phe Thr Pro Asn Ser Ile Asp Gly Thr Leu Val lie Asp Trp Tyr Gly 340 345 350 CAA CAG ATA GTT GGT TAT AAA GTA ATA CAG CCC GAC AAA ATT ACT GTG 1104 Gin GAA ATA GAA GAA CCC GAA CCT GAA ATA CCG GGC ACT GTT GGA ATA CAT 1440 Glu Ile Glu Glu Pro Glu Pro Glu Ile Pro Gly Thr Val Gly Ile His 465 470 475 480 TAT TCA TAC CTG ACA GGT TAT CCG GAC AAA ATG TTC AGA CCT GAA AAG 1488 Tyr Ser Tyr Leu Thr Gly Tyr Pro Asp Lys Met Phe Arg Pro Glu Lys 485 490 495 AGT ATT ACA AGA GCT GAA GCA GCC GTG ATT TTT GCA AAA CTT TTG GGA 1536 Ser Ile Thr Arg Ala Glu Ala Ala Val Ile Phe Ala Lys Leu Leu Gly 500 505 510 GCA AAC GAA AAT ACA AAG ATA AAC TAT AAT GTT TCA TAC ACC GAT GTT 1584 Ala Asn Glu Asn Thr Lys île Asn Tyr Asn Val Ser Tyr Thr Asp Val 515 520 525 GAC AGC TCC CAT TGG GCA AGT TGG GCA ATC AAA TTT GTA TCA TAC AAG 1632 Asp Ser Ser His Trp Ala Ser Trp Ala île Lys Phe Val Ser Tyr Lys 530 535 540 AAA CTG TTT ACC GGA TAT CCT GAT GGC TCG TTC AAG CCT AAT CAG AAT 1680 Lys Leu Phe Thr Gly Tyr Pro Asp Gly Ser Phe Lys Pro Asn Gîn Asn 545 550 555 560 ATA ACG AGA GCC GAA TTT TCA ACG GTT GTG TTT AAG CTT CTT GTA TCT 1728 île Thr Arg Ala Glu Phe Ser Thr Val Val Phe Lys Leu Leu Val Ser 565 570 575 GAG AAA GGT CTA AAA GAA GAA AAG ATT GAA AAG TCC AAG TTT GGT GAT 1776 Glu Lys Gly Leu Lys Glu Glu Lys Ile Glu Lys Ser Lys Phe Gly Asp 580 585 590 ACA AAG GGC CAC TGG GCA CAA CAG TTT ATT GAA CAG CTG TCA GAC CTT 1824 Thr Lys Gly His Trp Ala Gln Gln Phe Ile Glu Gîn Leu Ser Asp Leu 595 600 605 GGA TAC ATC AAC GGA TAT CCT GAT GGT ACA TTC AAG CCC AAC AAC AAT 1872 Gly Tyr Ile Asn Gly Tyr Pro Asp Gly Thr Phe Lys Pro Asn Asn Asn 610 615 620 ATC AAA CGA TCA GAA AGT GTT GCC CTG ATA AAC AGA GCT ATG GGA AGA 1920 île Lys Arg Ser Glu Ser Val Ala Leu Ile Asn Arg Ala Met Gly Arg 625 630 635 640 GGG CCT TTG CAT GGC GCA CCG CAG GTA TTC GAG GAT GTT CCT CAG ACA 1968 Gly Pro Leu His Gly Ala Pro Gîn Val Phe Glu Asp Val Pro Gln Thr 645 650 655 CAC TGG GCT TTC AAA GAT ATT GCA GAG GGC GTG CTC AAT CAC AGA TAC 2016 His Trp Alo Phe Lys Asp île Ala Glu Gly Val Leu Asn His Arg Tyr 660 665 670 AAA CTG GAC AAT GAG GGC AAA GAA CAA TTG CTG GAG ATA ATT GAT AAC 2064 Lys Leu Asp Asn Glu Gly Lys Glu Gln Leu Leu Glu Ile Ile As 675 680 685 DESCRIPTION DE LA SEQUENCE : SEQ ID NO: 4: SEQUENCE NUCLEOTIDIQUE DE LA PROTEINE CipA ATGAGAAAAGTCATCAGTATGCTCTTAGTTGTGGCTATGCTGACGACGATTTTTGCGGCGATGATAC CGC AGACAGTATCGGCGGCCACAATGACAGTCG AGATCGGCAAAGTTACAGCAGCCGTTGGATCAAAA OTAGA AATACCTATAACCCTGAAAGGAGTGCCATCCAAAGGAATGGCCAATTGCGACTTCGTATTGGGTTA TGAT CCAAATGTGCTGGAAGTAACAGAAGTAAAACCAGGAAGCATAATAAAAGATCCGGATCCTAGCAA GAGCT TTGATAGCGCAATATATCCGGATCGAAAGATGATTGTATTTCTGTTTGCAGAAGACAGTGGAAGAG GAAC GTATGCAATAACTCAGGATGGAGTATTTGCAACAATTGTAGCCACTGTCAAATCAGCTGCAGCGGC ACCG ATTACHTGCTTGAAGTAGGTGCATTTGCGGACAACGATTTAGTAGAAATAAGCACAACTTTTGTCG CGG GCGGAGTAAATCTTGGTAGTTCCGTACCGACAACACAGCCAAATGTTCCGTCAGACGGTGTGGTAG TAGA AATTGGCAAAGTTACGGGATCTGTTGGAACTACAGTTGAAATACCTGTATATTTCAGAGGAGTTCC ATCC AAAGGAATAGCAAACTGCGACTTTGTGTTCAGATATGATCCGAATGTATTGGAAATTATAGGGATA GATC CCGGAGACATAATAGTTGACCCGAATCCTACCAAGAGCTTTGATACTGCAATATATCCTGACAGAA AGAT AATAGTATTCCTGTTTGCGGAAGACAGCGGAACAGGAGCGTATGCAATAACTAAAGACGGAGTATT TGCA AAAATAAGAGCAACTGTAAAATCAAGTGCTCCGGGCTATATTACTTTCGACGAAGTAGGTGGA m GCAG ATAATGACCTOGTAGAACAGAAGGTATCATTTATAGACGGTGGTGTTAACGTTGGCAATGCAACAC CGAC CAAGGGAGCAACACCAACAAATACAGCTACGCCGACAAAATCAGCTACGGCTACGCCCACCAGGC CATCG GTACCGACAAACACACCGACAAACACACCGGCAAATACACCGGTATCAGGCAATTTGAAGGTTGA ATTCT ACAACAGCAATCCTTCAGATACTACTAACTCAATCAATCCTCAGTTCAAGGTTACTAATACCGGAA GCAG TGCAATTGATTTGTCCAAACTCACATTGAGATATTATTATACAGTAGACGGACAGAAAGATCAGAC CTTC TGGTGTGACCATGCTGCAATAATCGGCAGTAACGGCAGCTACAACGGAATTACTTCAAATGTAAAA GGAA CATTTGTAAAAATGAGTTCCTCAACAAATAACGCAGACACCTACCTTGAAATAAGCTTTACAGGCG GAAC TCTTGAACCGGGTGCACATGTTCAGATACAAGGTAGA m GCAAAGAATGACTGGAGTAACTATAC ACAG TCAAATGACTACTCATTCAAGTCTGCTTCACAGTTTGTTGAATGGGATCAGGTAACAGCATACTTGA ACG GTGTTCTTGTATGGGGTAAAGAACCCGGTGGCAGTGTAGTACCATCAACACAGCCTGTAACAACAC CACC TGCAACAACAAAACCACCTGCAACAACAAAACCACCTGCAACAACAATACCGCCGTCAGATGATCC GAAT GCAATAAAGATTAAGGTGGACACAGTAAATGCAAAACCGGGAGACACAGTAAATATACCTGTAAG ATTCA GTGGTATACCATCCAAGGGAATAGCAAACTGTGACTTTGTATACAGCTATGACCCGAATGTACTTG AGAT AATAGAGATAAAACCGGGAGAATTGATAGTTGACCCGAATCCTGACAAGAGCTTTGATACTGCAGT ATAT CCTGACAGAAAGATAATAGTATTCCTGTTTGCAGAAGACAGCGGAACAGGAGCGTATGCAATAACT AAAG ACGGAGTAHTGCTACGATAGTAGCGAAAGTAAAATCCGGAGCACCTAACGGACTCAGTGTAATCA AATT TGTAGAAGTAGGCGGATTTGCGAACAATGACCTTGTAGAACAGAGGACACAGTTCTTTGACGGTGG AGTA AATGTTGGAGATACAACAGTACCTACAACACCTACAACACCTGTAACAACACCGACAGATGATTCG AATG CAGTAAGGATTAAGGTGGACACAGTAAATGCAAAACCGGGAGACACAGTAAGAATACCTGTAAGA TTCAG CGGTATACCATCCAAGGGAATAGCAAACTGTGACTTTGTATACAGCTATGACCCGAATGTACTTGA GATA ATAGAGATAGAACCGGGAGACATAATAGTTGACCCGAATCCTGACAAGAGCTTTGATACTGCAGTA TATC CTGACAGAAAGATAATAGTATTCCTGTTTGCGGAAGACAGCGGAACAG GAGCGTATGCAATAACTA AAGA CGGAGTAHTGCTACGATAGTAGCGAAAGTAAAATCCGGAGCACCTAACGGACTCAGTGTAATCAA ATTT GTAGAAGTAGGCGGATTTGCGAACAATGACCTTGTAGAACAGAAGACACAGTTCTTTGACGGTGGA GTAA ATGTTGGAGATACAACAGAACCTGCAACACCTACAACACCTGTAACAACACCGACAACAACAGAT GATCT GGATGCAGTAAGGATTAAAGTGGACACAGTAAATGCAAAACCGGGAGACACAGTAAGAATACCTG TAAGA TTCAGCGGTATACCATCCAAGGGAATAGCAAACTGTGACTTTGTATACAGCTATGACCCGAATGTA CTTG AGATAATAGAGATAGAACCGGGAGACATAATAGTTGACCCGAATCCTGACAAGAGCTTTGATACTG CAGT ATATCCTGACAGAAAGATAATAGTATTCCTGTTTGCGGAAGACAGCGGAACAGGAGCGTATGCAAT AACT AAAGACGGAGTATTTGCTACGATAGTAGCGAAAGTAAAATCCGGAGCACCTAACGGACTCAGTGT AATCA AATTTGTAGAAGTAGGCOGAHTGCGAACAATGACCTTGTAGAACAGAAGACACAGTTCTTTGACG GTGG AGTAAATGTTGGAGATACAACAGAACCTGCAACACCTACAACACCTGTAACAACACCGACAACAA CAGAT GATCTGGATGCAGTAAGGATTAAAGTGGACACAGTAAATGCAAAACCGGGAGACACAGTAAGAAT ACCTG TAAGATTCAGCGGTATACCATCCAAGGGAATAGCAAACTGTGACTTTGTATACAGCTATGACCCGA ATGT ACTTGAGATAATAGAGATAGAACCGGGAGACATAATAGTTGACCCGAATCCTGACAAGAGCTTTGA TACT GCAGTATATCCTGACAGAAAGATAATAGTATTCCTGTTTGCAGAAGACAGCGGAACAGGAGCGTAT GCAA TAACTAAAGACGGAGTATTTGCTACGATAGTAGCGAAAGTAAAAGAAGGAGCACCTAACGGACTC AGTGT AATCAAATTTGTAGAAGTAGGCGGATTTGCGAACAATGACCTTGTAGAACAGAAGACACAGTTCTT TGAC GGTGGAGTAAATGTTGGAGATACAACAGAACCTGCAACACCTACAACACCTGTAACAACACCGAC AACAA CAGATGATCTGGATGCAGTAAGGATTAAAGTGGACACAGTAAATGCAAAACCGGGAGACACAGTA AGAAT ACCTGTAAGATTCAGCGGTATACCATCCAAGGGAATAGCAAACTGTGACTTTGTATACAGCTATGA CCCG AATGTACTTGAGATAATAGAGATAGAACCGGGAGAATTGATAGTTGACCCGAATCCTACCAAGAGC TTTG ATACTGCAGTATATCCTGACAGAAAGATGATAGTATTCCTGTTTGCGGAAGACAGCGGAACAGGAG CGTA TGCAATAACTGAAGATGGAGTATTTGCTACGATAGTAGCGAAAGTAAAATCCGGAGCACCTAACGG ACTC AGTGTAATCAAAHTGTAGAAGTAGGCGGATTTGCGAACAATGACCTTGTAGAACAGAAGACACAG TTCT HGACGGTGGAGTAAATGTTGGAGATACAACAGAACCTGCAACACCTACAACACCTGTAACAACAC CGAC AACAACAGATGATCTGGATGCAGTAAGGATTAAAGTGGACACAGTAAATGCAAAACCGGGAGACA CAGTA AGAATACCTGTAAGATTCAGCGGTATACCATCCAAGGGAATAGCAAACTGTGACTTTGTATACAGC TATG ACCCGAATGTACTTGAGATAATAGAGATAGAACCGGGAGACATAATAGTTGACCCG AATCCTGACA AGAG C m GATACTGCAGTATATCCTGACAGAAAGATAATAGTATTCCTGTTTGCAGAAGACAGCGGAAC GGGA GCGTATGCAATAACTAAAGACGGAGTATTTGCTACGATAGTAGCGAAAGTAAAAGAAGGAGCACC TAACG GACTCAGTGTAATCAAATTTGTAGAAGTAGGCGGATTTGCGAACAATGACCTTGTAGAACAGAAGA CACA GTTCTTTGACGGTGGAGTAAATGrFGGAGATACAACAGTACCTACAACATCGCCGACAACAACACC GCCA GAGCCGACGATAACTCCGAACAAGTTGACACTTAAGATAGGCAGAGCAGAAGGAAGACCTGGAGA CACGG TGGAAATACCGGTTAACTTGTATGGAGTACCTCAAAAAGGAATAGCAAGCGGTGACTTCGTAGTAA GCTA TGACCCGAATGTACTTGAGATAATAGAGATAGAACCGGGAGAATTGATAGTTGACCCGAATCCTAC CAAG AGCHTGATACTGCAGTATATCCTGACAGAAAGATGATAGTATTCCTGTTTGCGGAAGACAGCGGA ACAG GAGCGTATGCAATAACTGAAGATGGAGTATTTGCTACGATAGTAGCGAAAGTAAAAGAAGGAGCA CCTGA AGGATTCAGTGCAATAGAAAHTCTGAGTTTGGTGCATTTGCAGATAATGATCTGGTAGAAGTGGA AACT GACCTTATCAATGGTGGAGTACTTGTAACTAATAAACCTGTAATAGAAGGATATAAAGTATCCGGA TACA TTTTGCCAGACTTCTCCTTCGACGCTACTGTTGCACCACTTGTAAAGGCCGGATTCAAAGTTGAAAT AGT AGGAACAGAATTGTATGCAGTAACAGATGCAAACGGATACTTTGAAATAACCGGAGTACCTGCAA ATGCA AGCGGATATACATTGAAGATTTCAAGAGCAACTTACTTGGACAGAGTAATTGCAAATGTTGTAGTA ACGG GAGATACTTCAGTTTCAACTTCACAGGCTCCAATAATGATGTGGGTAGGAGACATAGTGAAAGACA ATTC TATCAACCTGTTGOACGTTGCAGAAGTTATCCGTTGCTTCAACGCTACTAAAGGAAGCGCAAACTA CGTA GAAGAACTTGACATTAATAGAAACGGCGCAATTAACATGCAAGACATAATGATTGTTCATAAGCAC TTTG GAGCTACATCAAGTGATTACGACGCACAGTAA SEQUENCE DE LA PROTEINE CipA MRKV!SMLLVVAMLTT!FAAMlPQTVSAATMTVEIGKVTAAVGSKVEIPITLKGvPSKGMANCDFvLGY DPNVLEVTEVKPGSIIKDPDPSKSFDSAIYPDRKMIVFLFAEDSGRGTYA ITQDGVFATI VATVKSAAAAP ITLLEVGAFADNDLVEISTTFVAGGVNLGSSVPTTQPNVPSDGVVVEIGKVTGSVGTTVEIPVYFRGVPS KGIANCDFVFRYDPNVLEIIGIDPGDIIVDPNPTKSFDTAIYPDRKIIVFLFAEDSGTGAYAITKDGVFAKIR ATVKSSAPGYITFDEVGGFADNDLVEQKVSFI DGGVNVGNATPTKGATPTNTATPTKSATATPTRPSVPT NTPTNTPANTPVSGNLKVEFYNSNPSDTTNSINPQFKVTNTGSSAIDLSKLTLRYYYTVDGQKDQTFWC DHAAIIGSNGSYNGITSNVKGTFVKMSSSTNNADTYLEISFTGGTLEPGAHVQ IQGRFAKNDWSNYTQS NDYSFKSASQFVEWDQVTAYLNGVLVWGKEPGGSVVPSTQPVTTPPATTKPPAHKPPAHIPPSDDPN AIKIKVDTVNAKPGDTVNIPVRFSGIPSKGIANCDFVYSYDPNVLEIIEIKPGELIVDPNPDKSFDTAVYPD RKIIVFLFAEDSGTGAYAITKDGVFATIVAKVKSGAPNGLSVIKFVEVGGFANNDLVEQRTQFFDGGVN VGDTTVPTTPTTPVTTPTDDSNAVRIKVDTVNAKPGDTVRIPVRFSGIPSKGIANCDFVYSYDPNVLEIIEI EPGDIIVDPNPDKSFDTAVYPDRKIIVFLFAEDSGTGAYAITKDGVFATIVAKVKSGAPNGLSVIKFVEVG GFANNDLVEQKTQFFDGGVNVGDTTEPATPTTPVTTPTTTDDLDAVRIKVDTVNAKPGDTVRIPVRFSG IPSKGIANCDFVYSYDPNVLEIIEIEPGDIIVDPNPDKSFDTAVYPDRKIIVFLFAEDSGTGAYAITKDGVFA TIVAKVKSGAPNGLSVIKFVEVGGFANNDLVEQKTQFFDGGVNVGDTIEPATPTTPVTTPTTTDDLDAV RIKVDTVNAKPGDTVRIPVRFSGIPSKGIANCDFVYSYDPNVLEIIEIEPGDIIVDPNPDKSFDTAVYPDRKI lVFLFAEDSGTGAYAlTKDGVFATlVAKVKEGAPNGLSVlKFVEVGG FANNDLVEQKTQFFDGGVNVGD TTEPATPTTPVTTPTTTDDLDAVRIKVDTVNAKPGDTVRIPVRFSGIPSKGIANCDFVYSYDPNVLEIIEIEP GELIVDPNPTKSFDTAVYPDRKMIVFLFAEDSGTGAYAITEDGVFATIVAKVKSGAPNGLSVIKFVEVGG FANNDLVEQKTQFFDGGVNVGDTTEPATPTTPVTTPEDDLDAVRIKVDTVNAKPGDTVRIPVRFSGIP SKGIANCDFVYSYDPNVLEIIEIEPGDIIVDPNPDKSFDTAVYPDRKIIVFLFAEDSGTGAYAITKDGVFATI VAKVKEGAPNGLSVIKFVEVGGFANNDLVEQKTQFFDGGVNVGDTTVPTTSPTTTPPEPTITPNKLTLKI GRAEGRPGDTVEIPVNLYGVPQKGIASGDFVVSYDPNVLEIIEIEPGELIVDPNPTKSFDTAVYPDRKMIV FLFAEDSGTGAYAITEDGVFATIVAKVKEGAPEGFSAIEISEFGAFADNDLVEVETDLINGGVLVTNKPVI EGYKVSGYILPDFSFDATVAPLVKAGFKVEIVGTELYAVTDANGYFEITGVPANASGYTLKISRATYLDR VIANVVVTGDTSVSTSQAPIMMWVGDIVKDNSINLLDVAEVIRCFNATKGSANYVEELDINRNGAINMQ DIMIVHKHFGATSSDYDAQ