LISTE DE SEOUENCES SEO ID NO:1 CARACTERISTIQUES DE LA SEQUENCE A) LONGUEUR : 600 nucléotides et 199 acides aminés B) TYPE : acide nucléique et protéine correspondante C) NOMBRE DE BRIN : simple D) CONFIGURATION : linéaire CARACTERISTIQUE ADDITIONNELLE A) NOM/CLE : prolactine de dinde (tPRL) DESCRIPTION DE SEQUENCE TTG CCA ATC TGC TCC AGT GGA TCT GTC AAC TGC CAA GTT TCC CTT GGG 48 Leu Pro Ile Cys Ser Ser Gly Ser Val Asn Cys Gln Val Ser Leu Gly 1 5 10 15 GAG CTT TTT GAT CGG GCA GTT AGA CTT TCA CAC TAC ATA CAC TTC CTC 96 Glu Leu Phe Asp Arg Ala Val Arg Leu Ser His Tyr Ile His Phe Leu 20 25 30 TCT TCA GAA ATT TTC AAT GAA TTT GAT GAA CGC TAT GCT CAG GGT CGG 144 Ser Ser Glu Ile Phe Asn Glu Phe Asp Glu Arg Tyr Ala Gln Gly Arg 35 40 45 GGT TTC ATT ACA AAA GCT GTT AAT GGC TGC CAC ACT TCC TCC TTA ACC 192 Gly Phe Ile Thr Lys Ala Val Asn Gly Cys His Thr Ser Ser Leu Thr 50 55 60 ACT CCT GAA GAT AAG GAG CAA ACT CAG CAG ATT CAT CAC GAA GAG CTA 240 Thr Pro Glu Asp Lys Glu Gln Thr Gln Gln Ile His His Glu Glu Leu 65 70 75 80 CTG AAT TTG ATA CTG GGA GTG CTG CGT TCC TGG AAT GAT CCC CTG ATC 288 Leu Asn Leu Ile Leu Gly Val Leu Arg Ser Trp Asn Asp Pro Leu Ile 85 90 95 CAT CTG GCC TCT GAA GTG CAA AGA ATC AAA GAA GCT CCA GAT ACC ATC 336 His Leu Ala Ser Glu Val Gln Arg Ile Lys Glu Ala Pro Asp Thr Ile 100 105 110 CTC TGG AAG GCT GTA GAG ATT GAG GAG CAA AAC AAG AGG CTT TTA GAA 384 Leu Trp Lys Ala Val Glu Ile Glu Glu Gln Asn Lys Arg Leu Leu Glu 115 120 125 GGA ATG GAG AAA ATC GTT GGG CGG ATT CAT TCT GGC GAT GCT GGA AAT 432 Gly Met Glu Lys Ile Val Gly Arg Ile His Ser Gly Asp Ala Gly Asn 130 135 140 GAA GTT TTC TCT CAG TGG GAC GGC CTT CCA TCC CTG CAA CTC GCT GAT 480 Glu Val Phe Ser Gln Trp Asp Gly Leu Pro Ser Leu Gln Leu Ala Asp 145 150 155 160 GAG GAC TCC AGA CTC TTT GCT TTT TAC AAC CTG CTG CAT TGC CTC CGC 528 Glu Asp Ser Arg Leu Phe Ala Phe Tyr Asn Leu Leu His Cys Leu Arg 165 170 175 AGA GAT TCC CAC AAA ATC GAC AAC TAT CTT AAA GTT TTG AAG TGC CGC 576 Arg Asp Ser His Lys Ile Asp Asn Tyr Leu Lys Val Leu Lys Cys Arg 180 185 190 CTA ATC CAT GAT AAC AAT TGC TAA 600 Leu Ile His Asp Asn Asn Cys * 195 199 SEO ID NO:2 CARACTERISTIQUES DE LA SEQUENCE A) LONGUEUR : 1281 nucléotides et 426 acides aminés B) TYPE : acide nucléique et protéine correspondante C) NOMBRE DE BRIN : simple D) CONFIGURATION: linéaire CARACTERISTIQUE ADDITIONNELLE A) NOM/CLE : protéine de fusion GST-tPRL CARACTERISTIQUE ADDITIONNELLE A) NOM/CLE : GST B) EMPLACEMENT - du nucléotide : 1 - au nucléotide : 682 B) EMPLACEMENT - de l'acide aminé : 1 - à l'acide aminé : 227 CARACTERISTIQUE ADDITIONNELLE : A) NOM/CLE : tPRL B) EMPLACEMENT - du nucléotide : 683 - au nucléotide . 181 B) EMPLACEMENT - de l'acide aminé : 228 - à l'acide aminé 426 DESCRIPTION DE SEQUENCE ATG TCC CCT ATA CTA GGT TAT TGG AAA ATT AAG GGC CTT GTG CAA CCC 48 Met Ser Pro Ile Leu Gly Tyr Trp Lys Ile Lys Gly Leu Val Gln Pro 1 5 10 15 ACT CGA CTT CTT TTG GAA TAT CTT GAA GAA AAA TAT GAA GAG CAT TTG 96 Thr Arg Leu Leu Leu Glu Tyr Leu Glu Glu Lys Tyr Glu Glu His Leu 20 25 30 TAT GAG CGC GAT GAA GGT GAT AAA TGG CGA AAC AAA AAG TTT GAA TTG 144 Tyr Glu Arg Asp Glu Gly Asp Lys Trp Arg Asn Lys Lys Phe Glu Leu 35 40 45 GGT TTG GAG TTT CCC AAT CTT CCT TAT TAT ATT GAT GGT GAT GTT AAA 192 Gly Leu Glu Phe Pro Asn Leu Pro Tyr Tyr Ile Asp Gly Asp Val Lys 50 55 60 TTA AGA CAG TCT ATG GCC ATC ATA CGT TAT ATA GCT GAC AAG CAC AAC 240 Leu Thr Gln Ser Met Ala Ile Ile Arg Tyr Ile Ala Asp Lys His Asn 65 70 75 80 ATG TTG GGT GGT TGT CCA AAA GAG CGT GCA GAG ATT TCA ATG CTT GAA 288 Met Leu Gly Gly Cys Pro Lys Glu Arg Ala Glu Ile Ser Met Leu Glu 85 90 95 GGA GCG GTT TTG GAT ATT AGA TAC GGT GTT TCG AGA ATT GCA TAT AGT 336 Gly Ala Val Leu Asp Ile Arg Tyr Gly Val Ser Arg Ile Ala Tyr Ser 100 105 110 AAA GAC TTT GAA ACT CTC AAA GTT GAT TTT CTT AGC AAG CTA CCT GAA 384 Lys Asp Phe Glu Thr Leu Lys Val Asp Phe Leu Ser Lys Leu Pro Glu 115 120 125 ATG CTG AAA ATG TTC GAA GAT CGT TTA TGT CAT AAA ACA TAT TTA AAT 432 Met Leu Lys Met Phe Glu Asp Arg Leu Cys His Lys Thr Tyr Leu Asn 130 135 140 GGT GAT CAT GTA ACC CAT CCT GAC TTC ATG TTG TAT GAC GCT CTT GAT 480 Gly Asp His Val Thr His Pro Asp Phe Met Leu Tyr Asp Ala Leu Asp 145 150 155 160 GTT GTT TTA TAC ATG GAC CCA ATG TGC CTG GAT GCG TTC CCA AAA TTA 528 Val Val Leu Tyr Met Asp Pro Met Cys Leu Asp Ala Phe Pro Lys Leu 165 170 175 GTT TGT TTT AAA AAA CGT ATT GAA GCT ATC CCA CAA ATT GAT AAG TAC 576 Val Cys Phe Lys Lys Arg Ile Glu Ala Ile Pro Gln Ile Asp Lys Tyr 180 185 190 TTG AAA TCC AGC AAG TAT ATA GCA TGG CCT TTG CAG GGC TGG CAA GCC 624 Leu Lys Ser Ser Lys Tyr Ile Ala Trp Pro Leu Gln Gly Trp Gln Ala 195 200 205 ACG TTT GGT GGT GGC GAC CAT CCT CCA AAA TCG GAT CTG GTT CCG CGT 672 Thr Phe Gly Gly Gly Asp His Pro Pro Lys Ser Asp Leu Val Pro Arg 210 215 220 GGA TCC TCC TTG CCA ATC TGC TCC AGT GGA TCT GTC AAC TGC CAA GTT 720 Gly Ser Ser Leu Pro Ile Cys Ser Ser Gly Ser Val Asn Cys Gln Val 225 230 235 240 TCC CTT GGG GAG CTT TTT GAT CGG GCA GTT AGA CTT TCA CAC TAC ATA 768 Ser Leu Gly Glu Leu Phe Asp Arg Ala Val Arg Leu Ser His Tyr Ile 245 250 255 CAC TTC CTC TCT TCA GAA ATT TTC AAT GAA TTT GAT GAA CGC TAT GCT 816 His Phe Leu Ser Ser Glu Ile Phe Asn Glu Phe Asp Glu Arg Tyr Ala 260 265 270 CAG GGT CGG GGT TTC ATT ACA AAA GCT GTT AAT GGC TGC CAC ACT TCC 864 Gln Gly Arg Gly Phe Ile Thr Lys Ala Val Asn Gly Cys His Thr Ser 275 280 285 TCC TTA ACC ACT CCT GAA GAT AAG GAG CAA ACT CAG CAG ATT CAT CAC 912 Ser Leu Thr Thr Pro Glu Asp Lys Glu Gln Thr Gln Gln Ile His His 290 295 300 GAA GAG CTA CTG AAT TTG ATA CTG GGA GTG CTG CGT TCC TGG AAT GAT 960 Glu Glu Leu Leu Asn Leu Ile Leu Gly Val Leu Arg Ser Trp Asn Asp 305 310 315 320 CCC CTG ATC CAT CTG GCC TCT GAA GTG CAA AGA ATC AAA GAA GCT CCA 1008 Pro Leu Ile His Leu Ala Ser Glu Val Gln Arg Ile Lys Glu Ala Pro 325 330 335 GAT ACC ATC CTC TGG AAG GCT GTA GAG ATT GAG GAG CAA AAC AAG AGG 1056 Asp Thr Ile Leu Trp Lys Ala Val Glu Ile Glu Glu Gln Asn Lys Arg 340 345 350 CTT TTA GAA GGA ATG GAG AAA ATC GTT GGG CGG ATT CAT TCT GGC GAT 1104 Leu Leu Glu Gly Met Glu Lys Ile Val Gly Arg Ile His Ser Gly Asp 355 360 365 GCT GGA AAT GAA GTT TTC TCT CAG TGG GAC GGC CTT CCA TCC CTG CAA 1152 Ala Gly Asn Glu Val Phe Ser Gln Trp Asp Gly Leu Pro Ser Leu Gln 370 375 380 CTC GCT GAT GAG GAC TCC AGA CTC TTT GCT TTT TAC AAC CTG CTG CAT 1200 Leu Ala Asp Glu Asp Ser Arg Leu Phe Ala Phe Tyr Asn Leu Leu His 385 390 395 400 TGC CTC CGC AGA GAT TCC CAC AAA ATC GAC AAC TAT CTT AAA GTT TTG 1248 Cys Leu Arg Arg Asp Ser His Lys Ile Asp Asn Tyr Leu Lys Val Leu 405 410 415 AAG TGC CGC CTA ATC CAT GAT AAC AAT TGC TAA 1281 Lys Cys Arg Leu Ile His Asp Asn Asn Cys * 420 426