Hengzongshu/ArticleAgent huggingface dataset:
The hypothalamus has an important role in the onset and maintenance of hypertension and stress responses. Rats with inherited stress-induced arterial hypertension (ISIAH), reproducing the human stress-sensitive hypertensive state with predominant involvement of the neuroendocrine hypothalamic-pituitary-adrenal and sympathoadrenal axes, were used for analysis of the hypothalamus transcriptome.

RNA-seq analysis revealed 139 genes differentially expressed in the hypothalami of hypertensive ISIAH and normotensive Wistar Albino Glaxo (WAG) rats. According to the annotation in databases, 18 of the differentially expressed genes (DEGs) were associated with arterial hypertension. The Gene Ontology (GO) functional annotation showed that these genes were related to different biological processes that may contribute to the hypertension development in the ISIAH rats.

The most significantly affected processes were the following: regulation of hormone levels, immune system process, regulation of response to stimulus, blood circulation, response to stress, response to hormone stimulus, transport, metabolic processes, and endocrine system development. The most significantly affected metabolic pathways were those associated with the function of the immune system and cell adhesion molecules and the metabolism of retinol and arachidonic acid. Of the top 40 DEGs making the greatest contribution to the interstrain differences, there were 3 genes (Ephx2, Cst3 and Ltbp2) associated with hypertension that were considered to be suitable for further studies as potential targets for the stress-sensitive hypertension therapy. Seven DEGs were found to be common between hypothalamic transcriptomes of ISIAH rats and Schlager mice with established neurogenic hypertension. The results of this study revealed multiple DEGs and possible mechanisms specifying the hypothalamic function in the hypertensive ISIAH rats. These results provide a basis for further investigation of the signalling mechanisms that affect hypothalamic output related to stress-sensitive hypertension development.
The liquid bridge rupture confined between two glass plates is experimentally investigated. High–resolution shadow technique is used for video registration.

Critical bridge diameters and distance between glass plates are found.
To resolve a prevailing nomenclatorial confusion present in the genus Aciagrion

A. tillyardi Laidlaw, 1919 is placed in synonymy of A. approximans (Selys, 1876).The neotype of "Pseudagrion approximans", a male specimen from Khasi Hills preserved in Coll.Selys Longchamps at RBINS, is designated to replace the lost holotype, which was an incomplete specimen of unknown provenance.Secondary sources suggest that Selys Longchamps himself had compared the present neotype specimen with the holotype.

A. tillyardi Laidlaw, 1919 is placed in synonymy of A. approximans (Selys, 1876)
Currently, a therapeutic drug based on recombinant antibodies for the prevention and treatment of tick-borne encephalitis virus (TBEV) is developed in ICBFM SB RAS, and the chimeric antibody ch14D5 is considered as one of the key components of this drug. It was previously shown that this antibody is directed to the domain D3 of the glycoprotein E of TBEV. It was previously shown that this antibody is able to protect mice from the European subtype of TBEV, strain “Absettarov”, and the presence of virus-neutralizing activity against the Far Eastern subtype of TBEV, strain 205 was also shown for this antibody. However, it remains unclear whether this antibody exhibits selectivity for different subtypes of TBEV. The aim of this study was to investigate the effect of amino acid sequence differences of recombinant D3 domains derived from the glycoprotein E of TBEV of the Far Eastern, Siberian and European subtypes on the binding of the protective antibody ch14D5 to these proteins. Using Western blot analysis and surface plasmon resonance, it was shown that ch14D5 antibody has the highest affinity (K D = 1.7±0.5 nM) for the D3 domain of the TBEV of the “Sofjin-Ru” strain belonging to the Far Eastern subtype of the virus. At the same time, the affinity of ch14D5 antibody for similar D3 proteins derived from “Zausaev”, “1528-99” and “Absettarov” strains of the Siberian and European subtypes of TBEV was noticeably lower (K D = 25±4, 300±50, 250±50 nM, respectively). In addition, information about the spatial arrangement of amino acid residues that are different for the studied recombinant proteins indicates that the epitope recognized by the ch14D5 antibody is in close proximity to the lateral ridge of D3 domain of E glycoprotein.
An experimental study of the temperature field on the surface of horizontal liquid layer (HFE-7100) evaporating into gas flow (Air) has been performed.

Temperature gradient of the gas-liquid interface has been measured with the help of Titanium 570M IR camera.

Shear stresses on gas-liquid interface induced by thermocapillary effect and inert gas flow have been defined.
The process e+e- --> p anti-p has been studied in the p anti-p mass range from threshold to 6.5 GeV/c^2 using the initial-state-radiation technique with both detected and undetected photon.

The analysis is based on 469 fb-1 of integrated luminosity collected with the BABAR detector at the PEP-II collider at e+e- center-of-mass energies near 10.6 GeV.
There has been remarkable recent progress in engineering high-dielectric constant two dimensional (2D) materials, which are being actively pursued for applications in nanoelectronics in capacitor and memory devices, energy storage, and high-frequency modulation in communication devices. Yet many of the unique properties of these systems are poorly understood and remain unexplored. Here we report a numerical study of hopping conductivity of the lateral network of capacitors, which models two-dimensional insulators, and demonstrate that 2D long-range Coulomb interactions lead to peculiar size effects. We find that the characteristic energy governing electronic transport scales logarithmically with either system size or electrostatic screening length depending on which one is shorter. Our results are relevant well beyond their immediate context, explaining, for example, recent experimental observations of logarithmic size dependence of electric conductivity of thin superconducting films in the critical vicinity of superconductor-insulator transition where a giant dielectric constant develops. Our findings mark a radical departure from the orthodox view of conductivity in 2D systems as a local characteristic of materials and establish its macroscopic global character as a generic property of high-dielectric constant 2D nanomaterials.
We characterize the mineralogy and geochemistry of Fe, Zn, Pb, Sb, As, Hg, Ag sulfide assemblages from mud masses currently extruded by the onshore South Sakhalin and Pugachev mud volcanoes (Sakhalin Island, Russia). Abundant Tl-rich pyrite in sulfide concentrate samples from the mud volcanoes coexists with common Hg- and Ge-rich sphalerite, as well as with sporadic boulangerite, robinsonite, bournonite, galena, realgar, metacinnabar, cinnabar, acanthite, and chalcopyrite. Sphalerites are remarkably enriched in Hg (locally reaching 27 wt%) and coupled zwith permanent abnormal enrichment in Ge (3008–3408 ppm). According to single-crystal XRD analyses and Raman spectroscopy, both Hg-poor and Hg-rich sphalerites are single-phase (Zn,Hg)Scub compounds. Pyrite is of diagenetic origin, judging by its trace-element chemistry, particular morphology, and heavy S isotope composition. Another assemblage, composed of Pb-Sb-(Hg) sulfide minerals and lesser As, Cu, Ag, and Bi compounds, results from hydrothermal alteration and is genetically related to Neogene volcano-sedimentary rocks found among the ejecta of the mud volcanoes. The composition of impurities in sphalerite from mud masses indicates crystallization at temperatures lower than ~100 °C, under the leaching effect of mud volcano waters.
We report new measurements of the cross sections for the production of D Dbar final states at the psi(3770) resonance.

Our data sample consists of an integrated luminosity of 2.93/fb of e+e- annihilation data produced by the BEPCII collider and collected and analyzed with the BESIII detector. We exclusively reconstruct three D0 and six D+ hadronic decay modes and use the ratio of the yield of fully reconstructed D Dbar events ("double tags") to the yield of all reconstructed D or Dbar mesons ("single tags") to determine the number of D0 D0bar and D+D- events, benefiting from the cancellation of many systematic uncertainties.

Combining these yields with an independent determination of the integrated luminosity of the data sample, we find the cross sections to be \sigma(e+e- --> D0 D0bar)=(3.615 +- 0.010 +- 0.038) nb and \sigma(e+e- --> D+D-)=(2.830 +- 0.011 +- 0.026) nb, where the uncertainties are statistical and systematic, respectively.
Now, the work aimed at extracting maximum information about archaeological objects using geophysical methods is relevant. The possibilities for this give us the integration of various geophysical methods.

In 2018–2019, electrical exploration works were carried out at the archaeological site Ust-Tartas mounds (Western Siberia). The sites for these works were selected based on magnetic survey data. The purpose of the research was to obtain additional information on the parameters of archaeological objects and to compare the possibilities of different geophysical methods. Several different magnetic anomalies were chosen for the studies. Methods of electromagnetic frequency sounding, electrotomography and georadiolocation were used.

We were able to refine the length, width, depth of objects, and their configuration. The type of objects studied (burial or pit, settlement complex) is defined. The most informative results, which complement the magnetic survey data, are obtained using the electrotomography method.
Aim . To study APOA5 genetic polymorphism in Caucasoid patients with familial hypercholesterolemia.

Methods . Sample of patient with familial hypercholesterolemia (43 unrelated Caucasoid persons) was formed using Dutch Lipid Clinic Network Criteria. Targeted sequencing of genome DNA was performed by NimbleGen SeqCap EZ Choice kit on pyrosequencer Roche Junior GS (Roche, Switzerland).

Results . In patients with familial hypercholesterolemia, 8 substitutions were identified in the APOA5 gene: rs2075291, rs3135506, rs2072560, rs2266788, rs3135507, rs34089864, rs619054, and rs651821, that are known to be associated with dyslipidemia. One novel substitution Ala169Asp was found. It is responsible for changing the charge of a domain for lipid droplets binding in the APOA5 protein. There were no differences in the frequencies of the ApoA5*2 intragenic haplotype, which has been recently reported to be associated with an increased triglyceride levels in patients with familial hypercholesterolemia and the population. Conclusion. Genetic variants ofAPOA5, common in patients with familial hypercholesterolemia, may be involved in the formation of the pathological phenotype of dyslipidemia. However, a more accurate assessment oftheir contribution is required to differentiate patients with familial hypercholesterolemia according to their triglycerides level.
The article attempts to compare the three female characters in the novel Doctor Zhivago to identify their role in the formation of the psychological image of the main character, as well as their main functions as characters in the plot structure of the novel. Distinguishing the terms “character” and “hero” as theoretical concepts, we seek to establish the complementarity of two methods: character analysis as a way of structuring the text, on the one hand, and phenomenological, which allows us to capture the uniqueness of a single hero, as well as its influence on the fate of other participants in the action. Taking into account the long-term work on Doctor Zhivago, the author of the article proceeds from the idea that along with autobiographical material in the characterization of three women – Tonya, Larisa, and Marina, B. Pasternak widely uses plot analogies with masterpieces of Russian and world literature, as well as certain allusions to biblical images. Important here is the principle of inseparability and non-confusion in the depiction of the fates of the three women in their relationship with the main character, associated with the Christian tradition. For the first time, the article introduces the essentiality– existentiality opposition to compare the main female figures. At the same time, it is important that the author, creating female images, uses a different measure of fabulousness associated with the vicissitudes in the life of the main character – the maximum in the relationship of Yuri and Lara and the minimum in relation to the hero with Marina. There is also a different degree of detail of the three images, the use of symbols in their characterization, and the symbolization as the main method of Marina’s characterization is noted. The author concludes that the outstanding Russian poet and novelist B. Pasternak shows a high degree of skill, penetrating the depths of female psychology. At the same time, while maintaining the principles of construction characteristic of the classical narrative, the writer, thanks to the special arrangement of the material, gives the novel features that bring it closer to the best examples of world literature of the twentieth century.
It is widely suggested that a eukaryotic mRNA typically contains one translation start site and encodes a single functional protein product. However, according to current points of view on translation initiation mechanisms, eukaryotic ribosomes can recognize several alternative translation start sites and the number of experimentally verified examples of alternative translation is growing rapidly.

Also, the frequent occurrence of alternative translation events and their functional significance are supported by the results of computational evaluations.

The functional role of alternative translation and its contribution to eukaryotic proteome complexity are discussed.
Seismogeological criteria for forecasting the gas content of the Aptian-Cenomanian sediments of Western Siberia are identified and substantiated in the study based on a comprehensive interpretation of seismic data, well log data, test results and petrophysical studies results. As a reference object we use the Jubilee oil and gas condensate field, which is located in the Nadym-Pur interfluve. The field contain the unique gas deposit in the Cenomanian reservoir horizon PK1, the Albian sand layer PK18 is productive too.

Based on the results of the research, seismogeological criteria for the gas-bearing forecast are formulated.

If there is a giant gas deposit in Cenomanian reservoir: 1) there is a reflectors formed on gas-water contacts (GWC) in seismic cross sections, amplitude characteristics of the reflector G, which is confined to the top of the Cenomanian complex, decrease; 2) time thickness (ΔT) increases, the interval velocities (Vint) decrease and amplitude-energy characteristics of the seismic record in the Aptian-Cenomanian complex decrease. The Aptian-Albian sheet gas reservoirs are displayed in seismic cross sections a jump in amplitudes of the seismic record, forming the 'bright spot' anomalies in the time sections.
The photoluminescence (PL) of the combined Ge/Si structures representing a combination of large (200–250 nm) GeSi disk-like quantum dots (nanodisks) and four-layered stacks of compact groups of smaller (30 nm) quantum dots grown in the strain field of nanodisks was studied.

The multiple increase in the PL intensity was achieved by the variation of parameters of vertically aligned quantum dot groups. The experimental results were analyzed on the basis of calculations of energy spectra, electron and hole wave functions.

It was found that the quantum dot arrangement in compact groups provides the effective electron localization in Δ x , y -valleys with an almost equal probability of finding an electron in the Si spacer and Ge barrier. As a result, the main channels of radiative recombination in the structures under study correspond to spatially direct optical transitions.
Different patterns of coat color pigmentation in dogs are produced by a sophisticated interaction of several genes. Understanding the mechanisms underlying the diversity of coat colors and their inheritance is important for professional breeders because it helps to predict the phenotypes of the progeny. Although genetics of the main coat colors in dogs is extensively studied, there are types of coat pigmentation that are not explained yet. Recently a new model connected the variants in ASIP gene promoters with different coat colors in dogs.

Here we used this model as a framework to investigate the genetics of the rare sesame coat color in Shiba Inu dogs. We determined the combination of two alleles of ASIP gene that determine sesame coat color.

This finding can be used by the breeders to produce the dogs with this rare coat color pattern. We also demonstrate the incomplete dominance between the ASIP alleles involved in sesame coat formation. These results are in good agreement with the new model explaining how different levels of ASIP gene expression affect the regulation of pigment synthesis in melanocytes.
This paper deals with an experimental study of the vortex structure of interacting coaxial swirling flows in an isothermal model of adaptive burner used for combustion of low-grade fuel. The working section consists of two consecutively connected tangential swirlers. Depending on the method of liquid flow supply to the second stage, both co-swirl and counter-swirl of two flows occur in the working section. In experiments, the effect of the flow swirl method on the resulting flow structure was studied with varying flow rates. Time-averaged distributions of axial and tangential velocity components along the entire region of the resulting flow were obtained using laser-Doppler anemometry. Experimental results have shown that counter-swirl is more preferable for the use in a two-stage burner because it promotes faster mixing of the burner jets of the first and second stages and more uniform filling of the internal volume of device.
Influence of perturbation frequency on mixing flow velocity and the varying flow structure in T-shape microchannels are investigated using laser induced fluorescence. The external disturbances of flow are created excitation system based on the piezoelectric actuator. Several increases in mixing efficiency were common to all regimes, but also some different features, were observed. For the stationary vortex flow regime, we obtained a decrease in mixing efficiency of 10% at a frequency of 650Hz. For the stationary asymmetric vortex flow regime, the mixing efficiency increases by 33% and 23% for the frequencies 500Hz and 800 Hz, respectively. For Re=400 and Re=300, the flow structures without disturbance are virtually identical; however, in the case of external influence for the quasiperiodic unsteady flow regime, the mixing efficiency decreases by 22% and 29% at distances of 1 and 5 calibres, respectively, for frequency of 1000 Hz.
Nonlinear interactions between different components of multiwavelength radiation are one of the main processes shaping properties of quasi-CW fiber lasers. In random fiber lasers, nonlinear influence may be more complicated, as there are no distinct longitudinal modes in radiation because of the random nature of the feedback.

In this Letter, we experimentally characterize internal correlations in the radiation of a multiwavelength random distributed feedback fiber laser. An analysis of Pearson correlation functions allows us to spatially locate the area over the fiber laser length in which correlations are more likely to occur.

This, in turn, leads us to the conclusion about the main mechanism of spectral correlations-the relative intensity noise transfer from the pump wave.
The results of numerical experiments upon modeling thermodynamic parameters such as value of pressure and compression of germanium and its alloys with gold are presented. The calculations were performed using the model TEC (thermodynamic equilibrium components). The model allows us to take into account the phase transition of germanium under shock-wave action. The interest in investigating of the compressibility for such materials is related both to the possibility of creating materials with the necessary properties and to the properties of the materials themselves. The results of calculations are compared with the known experimental results of different authors. The value of pressure and compression for shock wave loading of pure germanium and alloys with germanium as a component of various compositions are calculated.
A discrete model is constructed for calculating the Lame equation with complex boundary conditions.

The model is tested on an analytical solution. A complex boundary condition arises when a microcrack is specified on one of the boundaries.

Calculation of microcracks will enable better assessment of the relevance of the simulation and finding out which mechanisms will occur in the case of plasma flow heating in modern plasma and future thermonuclear installations.
The knowledge about specific mechanisms generating TP53 dysfunction in diffuse large B-cell lymphoma is limited.

The aim of the current study was to comprehensively explore TP53 gene variability resulting from somatic mutations, promoter methylation, and allelic imbalance in tumorous tissue of diffuse large B-cell lymphoma (DLBCL). DNA samples from 74 patients with DLBCL were used. Genomic DNA was isolated from paraffin blocks of lymph nodes or from extranodal biopsies of tumors by the phenol–chloroform extraction method with guanidine. Analysis of coding sequences of the TP53 gene was based on Sanger's direct sequencing method. The methylation status of the TP53 promoter was analyzed using by methylation-specific PCR on bisulfite-converted DNA. Assessment of the detected mutations was carried out in the IARC TP53 Database and the TP53 UMD mutation database of human cancer.

The mutations in regions coding for the DNA-binding domain were prevalent (95%). In the analyzed sample of patients, codons 275, 155, 272, and 212 were hotspots of mutations in the TP53 gene. In addition, functionally significant intron mutations (IVS6-36G > C and IVS5 + 43G > T) were detected. Instances of TP53 promoter methylation were observed only in a few samples of diffuse large B-cell lymphoma tissue. Furthermore, loss of heterozygosity was revealed only in the subgroup of patients with altered status of the gene (mutations were detected in five patients and promoter methylation in one case). Thus, the results suggest that there are two sequential events in the formation of diffuse large B-cell lymphoma in at least some cases. The first event is mutation or methylation of the TP53 promoter, leading to appearance of a cell with increased risk of malignant transformation. The second event is the loss of an intact allele of the gene; this change is necessary for tumorigenesis. We identified TP53 mutation patterns in a Russian cohort of patients with de novo DLBCL who were treated with R-CHOP and R-CHOP-like regimens and confirmed that TP53 mutation status is a valuable prognostic biomarker.