Hengzongshu/ArticleAgent huggingface dataset:
The cold atmospheric pressure plasma jet interaction with the bio-target is studied in the plasma experiment, 2D fluid model simulations, and with MTT and iCELLigence assays of the viability of cancer cells.

Plasma devices with cylindrical and plane geometries generating cold atmospheric plasma jets are developed and tested. The sequence of the streamers which forms the plasma jet is initiated with a voltage of 2.5–6.5 kV applied with the frequency 40 kHz. We suggest using the grounded substrate under the bio-target during the plasma jet treatment of cancer cells. The analysis of the measured plasma spectra and comparison of OH-line intensity for different voltages and gas flow rates allows us to find a range of optimal plasma parameters for the enhanced OH generation. The time-dependent viability is measured for human cell lines, A431 (skin carcinoma), HEK 293 (kidney embryonic cells), and A549 (human lung adenocarcinoma cells) after the plasma jet treatment.

It is shown, for the first time, that the use of the grounded substrate under the media with cells considerably amplifies the effect of plasma cancer cell treatment in vitro. The results with cell-based experiments (direct treatment) performed with various plasma jet parameters confirm the maximum efficiency of the treatment with the optimal plasma parameters.
In order to produce transgenic goats with hG-CSF, a total of 24 adult Saanen and 48 adult undefined breed goats were used as donors and recipients, respectively. Donors were estrus-synchronized with vaginal sponges and superovulated by a treatment with 200 mg FSH given twice daily in decreasing doses over 3 days starting 48 h before sponge removal. Ovulation was induced by injecting 100µg GnRH 36 h after sponge removal. The recipients also received an estrus synchronization treatment. Donors were mated with fertile Saanen bucks and, approximately 72 h after sponge removal, zygotes were recovered surgically by flushing oviducts. The recovered zygotes were briefly centrifuged to a reliable visualization of the pronuclei. The DNA construct containing hG-CSF gene flanked by goat and bovine alphas1-casein sequences was injected into pronuclei of 129 zygotes. The microinjected embryos (3-6 per female) were transferred to 27 recipients. Ten recipients became pregnant and 12 kids were born. One transgenic male founder was identified in the group of kids. This is the first report of a birth of a transgenic goat in Latin America.
The first IACT as part of the gamma and cosmic-ray experiment TAIGA was installed in the Tunka Valley near Lake Baikal in fall 2016. In the following months different systems of the telescope were put into test operation.

We started the commissioning phase of operating the telescope in time coincidence with the wide angle integrating air Cherenkov HiSCORE array, which for the time being comprises 28 working stations. We anticipate that the hybrid operation of the non-imaging and imaging telescopes will lead to a cost-efficient, highly sensitive detector operating in the energy range from 30-50 TeV till several PeV.

Here we want to report on the first test results of operating the prototype hybrid array. We will focus on the analysis of count rates and image size spectra for all events detected by the IACT and the joint events detected also by the HiSCORE array, comparing these with expectations from MC simulations.
Used as a kind of feed additive, p-arsanilic acid can pose a potential risk to organisms when abandoned in the environment.

The photodegradation of p-ASA was investigated under UV-C irradiation in the presence of persulfate (PS) in this work. The addition of PS facilitated the decomposition of p-ASA and notably, the presence of 50 mmol PS brought about a nearly complete mineralization after 3 h, while an insignificant total organic carbon (TOC) removal was observed under UV irradiation (λ = 254 nm) only. Experimental results proved that sulfate radical (SO4•−) was responsible for the promotion effect.

The cleavage of As-C bond released inorganic arsenic and caused the occurrence of various organic products, for example, hydroxybenzaldehyde, nitrobenzene, benzenediol sulfate, and biphenylarsinic acid. The application of PS with UV-C light may throw a light on thorough treatment for p-ASA containing wastewater.
The scent of receptive females as a signal to reproduction stimulates male mice to olfactory search of a potential breeding partner. This searching behavior is coupled with infection risk due to bacterial contamination of the fecal and urine scent marks.

Using bronchoalveolar lavage in a study on mice, we found the number of leukocytes to be significantly higher in male mice that were provided new portions of soiled bedding daily from female cages, in comparison with male mice that were kept in isolation from female scent. The number of leukocytes in blood was equal in both groups. However, monocytes were fewer in number in male mice exposed to female scent than in male mice isolated from female mice.

We hypothesized that sniffing of female soiled bedding induced the migration of immuno‐competent cells into airways as a possible adaptation to breeding‐related infection. Scent‐induced migration of leukocytes was accompanied by typical behavioral (increased sniffing activity and aggressiveness) and morphological (increase preputial glands and seminal vesicles) responses to olfactory sexual stimulus.
There has recently been great interest in the cryogels based on polyvinyl alcohol. Cryogel usage allows improving existing materials and creating new ones. Cryogel is completely safe, both for humans and for the soil. It protects the soil from drying out and erosion and enhances plant growth, making them more adaptive to the environment. Under the conditions of the Far North, cryogels might be used to strengthen building structures in order to prevent destruction.

There is a question of monitoring cryogel distribution in the treated environment. To do this, one needs to know the physical properties of cryogel and composites with ground on its basis. This work aims to study the electrical properties and pore space structure of the composites made from ground and cryogel at various temperatures and various amounts of freezing cycles.
Chromosomal inversions are important drivers of genome evolution. The Eurasian malaria vector Anopheles messeae has five polymorphic inversions. A cryptic species, An. daciae, has been discriminated from An. messeae based on five fixed nucleotide substitutions in the internal transcribed spacer 2 (ITS2) of ribosomal DNA. However, the inversion polymorphism in An. daciae and the genome divergence between these species remain unexplored.

In this study, we sequenced the ITS2 region and analyzed the inversion frequencies of 289 Anopheles larvae specimens collected from three locations in the Moscow region. Five individual genomes for each of the two species were sequenced.

We determined that An. messeae and An. daciae differ from each other by the frequency of polymorphic inversions. Inversion X1 was fixed in An. messeae but polymorphic in An. daciae populations. The genome sequence comparison demonstrated genome-wide divergence between the species, especially pronounced on the inversion-rich X chromosome (mean Fst = 0.331). The frequency of polymorphic autosomal inversions was higher in An. messeae than in An. daciae. We conclude that the X chromosome inversions play an important role in the genomic differentiation between the species. Our study determined that An. messeae and An. daciae are closely related species with incomplete reproductive isolation.
The Gaching ore occurrence within the Maletoyvayam ore field is located in the southwestern part of Kamchatka volcanic belt. The ore occurrence belongs to high-sulfidation epithermal type.

The sulfosalts of the fahlore-group (tennantite, tetrahedrites and goldfieldite) and enargite-groups (enargite, luzonite, famatinite) found in the gold mineralization of the Gaching ore occurrence.

The presence of Cu-rich and Se-bearing fahlores as well as enargite-group sulfosalts in ores indicate a high oxygen volatility at temperature less than 250º during their formation.
The method for observing the high-contrast and narrow-width resonances of electromagnetically induced absorption (EIA) in the Hanle configuration under counterpropagating light waves is proposed. We theoretically analyze the absorption of a probe light wave in presence of counterpropagating one with the same frequency as the function of a static magnetic field applied along the vectors of light waves, propagating in a vapour cell. Here, as an example, we study a "dark" type of atomic dipole transition Fg=1-->Fe=1 in D1 line of 87Rb, where usually the electromagnetically induced transparency (EIT) can be observed. To obtain the EIA signal one should proper chose the polarizations of light waves and intensities. In contrast of regular schemes for observing EIA signals (in a single travelling light wave in the Hanle configuration or in a bichromatic light field consisted of two travelling waves), the proposed scheme allows one to use buffer gas to significantly enhance properties of the resonance. Also the dramatic influence of atomic transition openness on contrast of the resonance is revealed, that gives great advantage in comparison with cyclic atomic transitions. The obtained results can be interesting in high-resolution spectroscopy, nonlinear and magneto-optics.
This work is dedicated to the history of Academy in Athens in early and late periods of its existence. I will first consider Academy in the time of Plato and then switch to the Athenian school of Neoplatonism ("the House of Proclus") in the period of late antiquity.

Using both archaeological and written evidences, fortunately sufficient in case of both of these institutions, I intend to draw a picture of their physical location and structure. Such an attitude towards the history, typical to the Annales School, will help us to place intellectual life of the ancients in proper everyday context.

There is one more reason why I have chosen these objects. One can say that these two institutions represent two different approaches to the way of life and the methods of education. On the one hand, one can see here the evolution of educational system from free discourses to more familiar for our educational system formal instructions. On the other hand, Plato's Academy reminds us of the contemporary tendency of organizing scientific life far from the densely populated cities in the form of university's campuses, which, to some extent, constitutes a return to the classical paradigm.
Anoxybacillus flavithermus strain 25 was isolated from a sediment sample from the Garga hot spring in the Barguzin Valley, Baikal Region, Russian Federation (54°19'3.72″N, 110°59'38.4″E).

The sequenced and annotated genome is 2,838,680 bp and encodes 3,009 genes.
The article is devoted to the development of a technique for determining the content of amylose and amylopectin, effective for potato starch. Since potato starch is an important renewable raw material for a number of industries, it is important to have a throughput approach that allows potato starch samples to be tested quickly for the content of its constituent polysaccharides for potato breeding for starch properties and for starch industrial application. The developed technique includes elements of previously disjointed procedures for dissolution and spectrophotometric determination of amylose in starch, and combines the following advantages: 1) starch samples dissolve in an organic solvent (0.5 % solution of lithium bromide in dimethyl sulfoxide (DMS)); 2) measurement of light absorption is performed at two wavelengths, 550 and 510 nm, and 3) the technique is adapted for use with a plate spectrophotometer. This procedure allows starch polysaccharides to avoid to avoid hydrolysis during dissolution, allows the precise spectrophotometric determination of the concentration of amylose complex with iodine in solution, and opens the possibility of using this technique for throughput phenotyping. Applying a certain dissolution procedure, it is also possible to avoid the formation of gelled starch clots in solutions for spectrophotometry, which is important for the preparation of solutions containing amylose and amylopectin in the same proportions as in the original starch. The technique was tested on starch isolated from potato tubers varieties Lina, Velikan, Golubizna, Favorit of domestic selection. The technique developed can be used for phenotyping starch of an extended set of potato varieties (determining the content and composition of amylose in potato starch samples) to identify "trait-genotype” associations.
Insects play an important role in biocenoses due to their abundance and wide (cosmopolitan) distribution. Many insects are crop pests. An effective pest control could be realized in case of proper species identification, which is usually managed by morphological analysis. Molecular methods allow to deep study of many issues of insect biology. In particular, traditional approach can not ordinary identify a species at all stages of their life cycle, whereas molecular methods can it. This review covers a wide range of issues related to the molecular genetic analysis of insects. In the first section we consider the methods of fixation and storage of insect specimens, as well as their impact on DNA quality. Further, we provide general information on population study design. Various schemes of DNA extraction, examples of both express techniques and more thorough protocols for DNA extraction and their purification are provided. In addition, methods of DNA isolation that allow to preserve a specimen integrity for further morphological studies are considered. The methods of DNA quality control are described in detail, that is important for PCR analysis. The last section provides various methods of PCR analysis, that we exemplify by studies aimed to elucidate both fundamental issues and practical problems.
Dynamic changes in the economic and social life of our country necessitate corresponding changes in the approaches to managing a modern University. One of these changes should be a new look at its corporate culture. The essence of the author's approach is to consider corporate culture as a key marketing asset of the University that impact on the strategic vision for the organization and operational management of all activities and units of the University. At the same time, the culture should be entrepreneurial in nature, which determines the University's ability to innovatively solve the tasks it faces and more successfully achieve the organization's strategic goals. However, still there is no scientific consensus reached in the literature about its features and the role in the success of the university. The purpose of this article is to substantiate the relevance of the formation of entrepreneurial corporate culture in the Russian universities. At the same time, it is proposed to consider entrepreneurial corporate culture as a key marketing asset of the organization, which is sure to reduce the negative effect of external environment and more successfully achieve the strategic goals of the university. The article presents the algorithm, mechanisms, and the main tools for the formation of entrepreneurial culture, given the specifics of this asset for an educational organization and the existing external environment. Theoretical significance of the proposed approach is a balanced approach to the university management, optimally combining conventional and unconventional for the educational environment methods to manage an educational organization. The practical significance of the study lies in the possibility of using the proposed methodology for the formation of entrepreneurial culture to improve the efficiency of the university management.
<span>В статье рассматриваются проблемы образования и накопления отходов в добывающем секторе и сопряженных отраслях, занятых переработкой его продукции. Данные сферы деятельности являются основным источником формирования отходов производства в России. Авторами рассчитаны показатели прямой и полной отходоемкости в данных отраслях, позволяющие оценить нагрузку на окружающую природную среду. Проанализированы материалы Стратегии развития промышленности по обработке, утилизации и обезвреживанию отходов производства и потребления на период до 2030 года. В статье рассматриваются основные направления совершенствования институциональных механизмов российского экологического законодательства, необходимые для успешной реализации целей и задач, изложенных в данном документе.</span>
A novel type of temporal and spatial self-organization in a heterogeneous catalytic reaction is described for the first time. Using in situ x-ray photoelectron spectroscopy, gas chromatography, and mass spectrometry, we show that, under certain conditions, self-sustained reaction-rate oscillations arise in the oxidation of propane over Ni foil because of reversible bulk oxidation of Ni to NiO, which can be observed even with the naked eye as chemical waves propagating over the catalyst surface.
RIL (product of PDLIM4 gene) is an actin-associated protein that has previously been shown to stimulate actin bundling by interacting with actin-cross-linking protein α-actinin-1 and increasing its affinity to filamentous actin.

Here, we report that the alternatively spliced isoform of RIL, denoted here as RILaltCterm, functions as a dominant-negative modulator of RIL-mediated actin reorganization. RILaltCterm is regulated at the level of protein stability, and this protein isoform accumulates particularly in response to oxidative stress. We show that the alternative C-terminal segment of RILaltCterm has a disordered structure that directs the protein to rapid degradation in the core 20 S proteasomes. Such degradation is ubiquitin-independent and can be blocked by binding to NAD(P)H quinone oxidoreductase NQO1, a detoxifying enzyme induced by prolonged exposure to oxidative stress. We show that either overexpression of RILaltCterm or its stabilization by stresses counteracts the effects produced by full-length RIL on organization of actin cytoskeleton and cell motility.

Taken together, the data suggest a mechanism for fine-tuning actin cytoskeleton rearrangement in response to stresses.
The mounting challenge of preserving the quality of life in an aging population directs the focus of longevity science to the regulatory pathways controlling healthspan. To understand the nature of the relationship between the healthspan and lifespan and uncover the genetic architecture of the two phenotypes, we studied the incidence of major age-related diseases in the UK Biobank (UKB) cohort. We observed that the incidence rates of major chronic diseases increase exponentially. The risk of disease acquisition doubled approximately every eight years, i.e., at a rate compatible with the doubling time of the Gompertz mortality law. Assuming that aging is the single underlying factor behind the morbidity rates dynamics, we built a proportional hazards model to predict the risks of the diseases and therefore the age corresponding to the end of healthspan of an individual depending on their age, gender, and the genetic background. We suggested a computationally efficient procedure for the determination of the effect size and statistical significance of individual gene variants associations with healthspan in a form suitable for a Genome-Wide Association Studies (GWAS). Using the UKB sub-population of 300,447 genetically Caucasian, British individuals as a discovery cohort, we identified 12 loci associated with healthspan and reaching the whole-genome level of significance. We observed strong (| ρ g | > 0.3) genetic correlations between healthspan and the incidence of specific age-related disease present in our healthspan definition (with the notable exception of dementia). Other examples included all-cause mortality (as derived from parental survival, with ρ g = −0.76), life-history traits (metrics of obesity, age at first birth), levels of different metabolites (lipids, amino acids, glycemic traits), and psychological traits (smoking behaviour, cognitive performance, depressive symptoms, insomnia). We conclude by noting that the healthspan phenotype, suggested and characterized here, offers a promising new way to investigate human longevity by exploiting the data from genetic and clinical data on living individuals.
SR micro-XRF is an x-ray spectral technique for elemental analysis of samples of various natures with a high spatial resolution (0.01 – 50 mkm). The deconvolution problem is needed to solve in order to improve the spatial resolution. In this work the potential to increase this resolution 1.5 time by simplest variational Tikhonov regularization algorithms is showed experimentally. The work was performed for the Elemental Analysis Station at the VEPP-3 storage ring.
The article presents the results of a new approach application for oil well galvanic and induction resistivity logs simulation to enhance the efficiency of geological environment parameters evaluation and to speed up the interpretation. The use of modern machine learning technologies allows us to create algorithms for resistivity logs simulation in high-detailed two-dimensional anisotropic geoelectric models. The developed algorithms are characterized by a qualitatively new level of performance compared to the approaches used today.
A metallic nanoparticle (NP) located near the film acts as a nanoantenna. Surface plasmons, excited by light in a particle and in a film, transform the electromagnetic field, confine it and strongly amplify it in the gap between the particle and the film.

The enhancement of the field depends on many factors: the size and shape of NP, the permeability of all materials, the wavelength of light, the thickness of the film, the angle of the light beam and very much from the gap between the particle and the film.

The amplification of the field in a narrow gap (~ 1 nm) can be 10^3 or more and the enhancement of Raman radiation can reach enormous values of ~ 10^10–10^11.
So far, little is known about the molecular mechanisms of amyotrophic lateral sclerosis onset and progression caused by SOD1 mutations. One of the hypotheses is based on SOD1 misfolding resulting from mutations and subsequent deposition of its cytotoxic aggregates. This hypothesis is complicated by the fact that known SOD1 mutations of similar clinical effect could be distributed over the whole protein structure. In this work, a measure of hydrogen bond stability in conformational states was studied with elastic network analysis of 35 SOD1 mutants. Twenty-eight hydrogen bonds were detected in nine of 35 mutants with their stability being significantly different from that with the wild-type. These hydrogen bonds were formed by the amino acid residues known from the literature to be located in contact between SOD1 aggregates. Additionally, residues disposed between copper binding sites of both protein subunits were found from the models to form a stiff core, which can be involved in mechanical impulse transduction between these active centres. The modelling highlights that both stability of the copper binding site and stability of the dimer can play an important role in ALS progression.
Dynamics of a single vapor cavity, moving under gravity in a vertical tube of a small diameter, has been studied. The experiment was carried out in water and aqueous LiBr solution in a tube with the length of 400 mm and diameter of 16 mm. Experiments have shown that dynamics of the rising vapor cavity after-the-fact boiling-up of aqueous LiBr solution in a vertical tube of a small diameter for some initial conditions differs qualitatively from dynamics of a gas bubble rising in a pool. Dynamics of the vapor cavity in aqueous LiBr solution and in water demonstrates quantitative but not qualitative differences. During bubble rising in a small diameter tube to time dependence for volume and mass of the vapor bubble, the regimes with pulsations were detected.