Hengzongshu/ArticleAgent huggingface dataset:
Objective: to compare the morphological and functional properties of mesenchymal stem cells from mammary tissues and chemically-induced mammary tumor tissues.

material and methods. The study included 25 female Wistar rats. In 20 rats, mammary carcinoma was induced by intramammary injection of N-methyl-N-nitrosourea after estrus synchronization with chorionic gonadotropin. The control group consisted of 5 rats. Mammary carcinoma was verified histologically and immunohistochemically. To examine, whether the cells isolated from normal tissue and tumor tissue belonged to mesenchymal stem cells, FACS Canto II flow cytofluorometer was used. The functional properties of mesenchymal stem cells were evaluated in MTT assay by the level of nitric oxide production in normal and by hydrogen peroxide-induced hypoxia. The levels of prolactin, luteinizing hormone and estradiol E2 in urine were studied using solid-phase enzyme-linked immunosorbent assay.

results. Chemically-induced mammary tumor according to histological and immunohistochemical studies corresponded to luminal B type breast cancer in humans. In rats that developed mammary tumors, the urine prolactin levels after synchronization of estrus were increased. In rats that did not develop tumors, the levels of prolactin and luteinizing hormone were decreased, but the levels of estradiol E2 were increased. More mesenchymal stem cells with CD45-/CD90+phenotype were obtained from the breast tumor tissue. Mesenchymal stem cells from tumor tissue showed increased proliferative potential and were more resistant to hypoxia. conclusion. Tumor- associated mesenchymal stem cells having high proliferative potential and resistance to hypoxia were obtained from chemically-induced mammary tumor tissue. Morphologic and functional differences in mesenchymal stem cells obtained from mammary breast tissue and tumor tissue require further studies.
Boron neutron capture therapy (BNCT) is an anticancer modality realized through 10B accumulation in tumor cells, neutron irradiation of the tumor, and decay of boron atoms with the release of alpha-particles and lithium nuclei that damage tumor cell DNA. As high-LET particle release takes place inside tumor cells absorbed dose calculations are difficult, since no essential extracellular energy is emitted.

We placed gold nanoparticles inside tumor cells saturated with boron to more accurately measure the absorbed dose. T98G cells accumulated ~50 nm gold nanoparticles (AuNPs, 50 µg gold/mL) and boron-phenylalanine (BPA, 10, 20, 40 µg boron-10/mL), and were irradiated with a neutron flux of 3 × 108 cm−2s−1. Gamma-rays (411 keV) emitted by AuNPs in the cells were measured by a spectrometer and the absorbed dose was calculated using the formula D = (k × N × n)/m, where D was the absorbed dose (GyE), k—depth-related irradiation coefficient, N—number of activated gold atoms, n—boron concentration (ppm), and m—the mass of gold (g). Cell survival curves were fit to the linear-quadratic (LQ) model.

We found no influence from the presence of the AuNPs on BNCT efficiency. Our approach will lead to further development of combined boron and high-Z element-containing compounds, and to further adaptation of isotope scanning for BNCT dosimetry.
The optical properties of ordered arrays of silicon nanopillars (Si NPs) were investigated. Electron Beam Lithography (EBL) followed by Inductively Coupled Plasma Reactive Ion Etching (ICP-RIE) was used for Si NPs fabrication. Si NPs were chemically and electrically passivated through the deposition of TiONx nanolayer. The silicon nanopillars were characterized by using scanned electron microscopy (SEM), high resolution transmission electron microscopy (HRTEM). We demonstrate that a various colors can be obtained by exploiting the resonant light scattering properties of individual Si NP. In addition the low temperature photoluminescence from Si NPs was investigated. The peak photoluminescence energy was observed at 0.83 μm and 1.14 μm. Raman scattering enhancement was found too.
In the optical spectral region, an insignificant shift of the phonon mode positions with a variation of the Sn content prevents determination of Sn concentration relying only on the optical phonons behavior. The application of the elastic continuum model with the linear approximation of sound velocity via Sn content in GeSiSn layers fails to explain the experimental results.

We apply Raman spectroscopy to study the phonon spectrum in periodical GeSiSn/Si nanostructures with Sn concentration varied from 0 to 20%. In the acoustic region, we observe the doublets of the folded acoustic phonons, the spectral positions of which undergo the low-frequency shift with increasing the Sn content.

This indicates a nonlinear Sn concentration dependence of sound velocity in GeSiSn layers which describes well the positions of the folded acoustic phonons.
Poly(alkylphosphanylborane) mit hohen Molekülmassen sind derzeit auf herkömmliche Weise durch metallkatalysierte Dehydropolymerisation nicht zugänglich, als metallfreie Alternative bietet sich aber die milde Thermolyse eines Lewis-Base-stabilisierten Phosphanylborans an. In der Zuschrift auf S. 13986 ff. beschreiben M. Scheer, I. Manners et al. die erfolgreiche Synthese von Poly(tert-butylphosphanylboran) nach dieser vielversprechenden Strategie. Poly(alkylphosphanylborane) mit hohen Molekülmassen sind derzeit auf herkömmliche Weise durch metallkatalysierte Dehydropolymerisation nicht zugänglich, als metallfreie Alternative bietet sich aber die milde Thermolyse eines Lewis-Base-stabilisierten Phosphanylborans an. In der Zuschrift auf S. 13986 ff. beschreiben M. Scheer, I. Manners et al. die erfolgreiche Synthese von Poly(tert-butylphosphanylboran) nach dieser vielversprechenden Strategie. Leitstrukturen C-H-Aktivierungen Zellsignalisierung
Aim . To study the association of new polymorphisms with sudden cardiac death (SCD) in men.

Material and methods . The SCD group (n=278) was formed using guidelines of the World Health Organization and the European Society of Cardiology. The autopsy material was taken from the men who died suddenly outside the prevention and treatment facilities who underwent medicolegal examination according to a standard protocol. The average age in the SCD group was 53,2±8,7 years. The control group (n=274) was selected by sex and age from the DNA bank formed during the international studies of MONICA and HAPIEE. The mean age of men in the control group was 53.±8,3 years. Genomic DNA was separated from myocardial tissue and venous blood by the method of phenol-chloroform extraction. Rs10503929, rs7121, rs6730157, rs11720524, rs7737692, rs7521023 polymorphisms were tested using real-time PCR. The insertion- deletion polymorphisms rs.0692285 and rs39.7 were genotyped by PCR with flanking primers followed by polyacrylamide gel electrophoresis. Statistical analysis was performed using the SPSS 13.0 software package.

Results . By comparison the frequencies of rs7121 genotypes in the studied groups of Novosibirsk, an increase in the proportion of CC genotype carriers in the group with SCD (OR=.6, 95% CI 1,1-2,2; p=0,025) was found. The carriage of the rs.0692285 ID genotype reduces the risk of SCD (OR=0,6, 95% CI 0,4-0,9; p=0,0M). A carriage of the rs39.7 ID genotype increases the risk of SCD (OR=.5, 95% CI 1,1-2,2; p=0,020). Conclusion . The rs7121 of the GNAS gene, the rs.0692285 of the RYR2 gene, and the rs39.7 of the COL1A2 gene are associated with SCD. Rs10503929, rs6730157, rs11720524, rs7737692, rs7521023 polymorphisms do not associate with SCD in the men of Novosibirsk.
SEIR (Susceptible - Exposed - Infected - Recovered) approach is a classic modeling method that has frequently been applied to the study of infectious disease epidemiology. However, in the vast majority of SEIR models and models derived from them transitions from one population group to another are described using the mass-action law which assumes population homogeneity. That causes some methodological limitations or even drawbacks, particularly inability to reproduce observable dynamics of key characteristics of infection such as, for example, the incubation period and progression of the disease’s symptoms which require considering different time scales as well as probabilities of different disease trajectories.

In this paper, we propose an alternative approach to simulate the epidemic dynamics that is based on a system of differential equations with time delays to precisely reproduce a duration of infectious processes (e.g. incubation period of the virus) and competing processes like transition from infected state to the hospitalization or recovery. The suggested modeling approach is fundamental and can be applied to the study of many infectious disease epidemiology. However, due to the urgency of the COVID-19 pandemic we have developed and calibrated the delay-based model of the epidemic in Germany and France using the BioUML platform. Additionally, the stringency index was used as a generalized characteristic of the non-pharmaceutical government interventions implemented in corresponding countries to contain the virus spread.

The numerical analysis of the calibrated model demonstrates that adequate simulation of each new wave of the SARS-CoV-2 virus spread requires dynamic changes in the parameter values during the epidemic like reduction of the population adherence to non-pharmaceutical interventions or enhancement of the infectivity parameter caused by an emergence of novel virus strains with higher contagiousness than original one. Both models may be accessed and simulated at https://gitlab.sirius-web.org/covid-19/dde-epidemiology-model utilizing visual representation as well as Jupyter Notebook.
Background Marine protists are an important part of the ocean ecosystem. They may possess unique sets of biosynthetic pathways and, thus, be promising model organisms for metabolic engineering for producing substances for the pharmaceutical, cosmetic, and perfume industries. Currently, full-genome data are available just for a limited number of protists hampering their use in biotechnology.

Methods We characterized the morphology of a new cultured strain of Thraustochytriaceae isolated from the Black Sea ctenophore Beroe ovata using phase-contrast microscopy. Cell culture was performed in the FAND culture medium based on fetal bovine serum and DMEM. Phylogenetic analysis was performed using the 18S rRNA sequence. We also conducted a transcriptome assembly and compared the data with the closest species.

Results The protist belongs to the genus Thraustochytrium based on the 18S rRNA sequence analysis. We designated the isolated protist as T. aureum ssp. strugatskii . The closest species with the genome assembly is Schizochytrium aggregatum . Transcriptome analysis revealed the majority of the fatty acid synthesis enzymes. Conclusion Our findings suggest that the T. aureum ssp. strugatskii is a promising candidate for biotechnological use. Together with the previously available, our data would allow the establishment of an accurate phylogeny of the family Thraustochytriaceae. Also, it could be a reference point for studying the evolution of the enzyme families.
Chronic musculoskeletal pain affects all aspects of human life. However, mechanisms of its genetic control remain poorly understood. Genetic studies of pain are complicated by the high complexity and heterogeneity of pain phenotypes. Here, we apply principal component analysis to reduce phenotype heterogeneity of chronic musculoskeletal pain at four locations: the back, neck/shoulder, hip, and knee. Using matrices of genetic covariances, we constructed four genetically independent phenotypes (GIPs) with the leading GIP (GIP1) explaining 78.4% of the genetic variance of the analyzed conditions, and GIP2–4 explain progressively less. We identified and replicated five GIP1-associated loci and one GIP2-associated locus and prioritized the most likely causal genes. For GIP1, we showed enrichment with multiple nervous system-related terms and genetic correlations with anthropometric, sociodemographic, psychiatric/personality traits and osteoarthritis. We suggest that GIP1 represents a biopsychological component of chronic musculoskeletal pain, related to physiological and psychological aspects and reflecting pain perception and processing.
The characteristics of burning of substandard liquid hydrocarbons in a direct-flow evaporative burner with controlled forced jet feeding of superheated steam are studied by the example of waste transmission automobile oil. The influence of the steam regime parameters on the temperature distribution in the flame, the thermal power and the composition of the combustion products is determined. The regimes providing simultaneously a low concentration of CO and NOx and a high power of heat release have been found.
We report an experimental study of an all-optical three-photon-absorption resonance (N -resonance) and discuss its potential application as an alternative to atomic clocks based on coherent population trapping (CPT).
To identify associations of fatty acids (FAs) with the antioxidant enzymes in the blood of men with coronary atherosclerosis and ischemic heart disease (IHD).The study included 80 patients: control group-20 men without IHD, the core group-60 men with IHD. The core group was divided into subgroups: subgroup A-with the presence of vulnerable atherosclerotic plaques, subgroup B-with the absence of vulnerable atherosclerotic plaques. We analyzed the levels of FAs, free radicals, superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) in the blood.Patients with IHD, compared with the control group: (1) had higher levels of SOD, CAT, myristic, palmitic, palmitoleic, and octadecenoic FAs; (2) had lower levels of GPx, α-linolenic, docosapentaenoic, docosahexaenoic, and arachidonic FAs. In subgroup A there were found: (1) negative associations of SOD-with linoleic, eicosatrienoic, arachidonic, eicosapentaenoic, docosapentaenoic and docosahexaenoic FAs, positive associations-with palmitic acid; (2) positive correlations of CAT level with palmitoleic and stearic acids; (3) negative associations between of GPx and palmitic, palmitoleic, stearic and octadecenoic FAs.Changes in the levels of antioxidant enzymes, and a disbalance of the FAs profile, probably indicate active oxidative processes in the body and may indicate the presence of atherosclerotic changes in the vessels.
The article presents the results of internal consistency and internal structure analysis on a sample of 184 subjects of the four most widely used questionnaires of tolerance / intolerance to ambiguity: the Intolerance to Ambiguity Scale (IAS) by S. Badner, Multiple Stimulus Types Ambiguity Tolerance Scale (MSTAT-I and MSTAT-II) D. McLane and the Tolerance-intolerance of ambiguity new questionnaire (TAN) by T. V. Kornilova. The IAS subscales showed low internal consistency, the Tolerance-intolerance of ambiguity new questionnaire scale showed satisfactory internal consistency and the MSTAT-I and MSTAT-II scales showed high internal consistency. Confirmatory factor analysis confirmed the five-factor model of MSTAT-I, the other models were not confirmed on our data. Exploratory factor analysis revealed unsatisfactory internal structure of the IAS, TAN, MSTAT-II. The results of the study are discussed.
In the present paper, results of an experimental and numerical study of gas flows in radial micro-nozzles are reported. The radial micro-nozzle consists of two disks spaced apart by a less than one millimeter distance. To the inlet of the radial nozzle, a gas under high pressure is supplied; through the nozzle, the pressurized gas is ejected into ambient space.

In the present study, we analyzed the flows in which the gas at the inlet to the micro-nozzle had a supersonic velocity.

It was shown that, on the condition that the micro-nozzle width was smaller than some critical value, then, due to the wall friction force, a pseudo-shock arose in the micro-nozzle. In the pseudo-shock, the gas underwent deceleration to a subsonic velocity. A satisfactory agreement between the calculated and experimental data was achieved.
The model takes into account oscillations of the bubble surface and the attached to it solid cylindrical particle in infinite volume of ideal incompressible liquid. The capillary force holding the particle on the bubble is due to the shape of the meniscus surface, which determines the wetting edge angle.

A model of the interaction of a spherical gas bubble and a rigid particle is derived as a coupled system of second-order differential equations using Lagrangian mechanics. The series with respect Legendre polynomials is used to present small axisymmetric oscillations of the particle-bubble system. Potential and kinetic energies are expressed through coefficients of this series. Particle adhesion condition to bubble surface is implemented through Lagrange multipliers.

The dependence of the particle size and its density is demonstrated as a result of the numerical integration of the resulting dynamic system of differential equations.
A significant feature of the knowledge economy is an updated education system aimed at the production of human capital capable of innovative activity in all sectors. The article deals with the scientific potential of the higher school in Russia and its ability to create the knowledge economy to form competitive Russian economy in the world market. There are interesting studies of foreign and Russian scientists on this topic. Many serious problems of higher education of the Russian Federation are still not resolved up to the present day. It refers to most higher education institutions that have not received the status of national research or federal institution, but they continue to carry out their mission of training young specialists for production under years of underfunding. The "optimization" process in education has made such universities weak, the profession of a teacher has become less attractive, the teaching staffs have an average age above 60 years, and it is deprived of renewal possibility. The scientific potential of Russia, including the higher education institutions, is significant and inexhaustible. The state should only use it correctly and rationally, take care of human resources, provide social protection for scientists and teachers. All these will attract young researchers to academic and university science, it will ensure the success of the knowledge economy in the country.
The paper presents the experience of modeling supersonic jets of rarefied gases expanding into a vacuum or highly rarefied medium of any given gas composition, as well as interacting flows from nozzle blocks on experimental installations developed at Novosibirsk State University. Examples of visualization of complex sparse flows, results of measurements by electron-beam spectroscopy and molecular-beam mass spectrometry of the density and flows composition under different flow conditions are given. The obtained experimental data validation problems and natural conditions modeling possibilities on small-sized laboratory installations, model and real flows conditions and parameters of similarity are discussed.
In this paper the influence of dispersed catalysts obtained in situ based on various metals (Mo, Ni, Fe, Co) has been studied on the yield and properties of liquid products in a slurry-reactorat a temperature of 425 °С and a pressure of 20 MPa. The most active catalytic compositions for the process of catalytic steam cracking of oil (Mo and Mo-Fe) were selected. In the presence of dispersed Mo and Mo-Fe catalysts the samples of the semisynthetic oils were produced. In the rectification process the gasoline, diesel, oil and residual fractions were allocated. The selected fractions were analyzed by physicochemical methods for a wide range of performance in comparison with the initial heavy oil. The efficiency of Mo-Fe catalysts for obtaining semisynthetic oil with an increased content of light fractions (i.b.p. – 360 °С) during the catalytic steam cracking of heavy oil was shown
The REE distribution in 700–660-Mа zircon is consistent with that of the typical zircon from syenites (Belousova et al., 2002)...The trace-element distribution pattern of zircon from kamaphorites is very similar to the geochemical characteristics of zircon from Tiksheozero carbonatites (Tichomirowa et al., 2013)...support the zircon (Vladykin et al., 2014) and pyrochlore (Antonov et al., 2017) age dates determined following the U-Pb method and those of biotite obtained following the 40Ar-39Ar method (Vladykin et al., 2014).

Zircon from Tomtor syenites and kamaphorites was dated following the U-Pb method (SHRIMP-II), and the distribution of trace and rare-earth elements (REE) was studied at the same zircon point using an ion microprobe.

The main zircon population from syenites was dated at 402 ± 7 Ma, while the age range of single zircon grains was 700–660 Mа...This is the first isotope-geochemical study of zircon from kamaphorites, and the U-Pb age of ca. 400 Mа is within the error limits with of the main zircon population from syenites...The new age dates for Tomtor syenites and kamaphorites, consistent with 700–660 Mа and ca. 400 Mа events, support the zircon and pyrochlore age dates.
For the first time, orthogonally substituted azole-carboxylate adamantane ligands were synthesized and used for preparation of coordination polymers. The angle-shaped ligands were prepared by the reaction of 1-adamantanecarboxylic acid and azoles (1H-1,2,4-triazole, 3-methyl-1H-1,2,4-triazole, 3,5-dimethyl-1H-1,2,4-triazole, 1H-tetrazole, 5-methyl-1H-tetrazole) in concentrated sulfuric acid. Variation of the solvent and substituents in azole rings allowed to prepare both 1D and 2D copper(II) and nickel(II) coordination polymers, [Cu2(trzadc)4(H2O)0.7]∙DMF∙0.3H2O, [Cu(trzadc)2(MeOH)]∙MeOH, [Ni(trzadc)2(MeOH)2] and [Cu2(mtrzadc)3(MeOH)]+NO3- (trzadc-3-(1,2,4-triazol-1-yl)-adamantane-1-carboxylic acid; mtrzadc-3-(3-methyl-1,2,4-triazol-1-yl)-adamantane-1-carboxylic acid) which were structurally characterized by single crystal X-ray diffraction. Complex [Cu(trzadc)2(MeOH)]∙MeOH was shown to act as a catalyst in the Chan-Evans-Lam arylation reaction.
Nowadays, secondary education provides a broad variety of different compulsory courses giving a solid basis for further student's progress at the university. However, there is an opinion that in this system we are losing the sense of adventures, discoveries and research. Now the main question is if we can efficiently combine compulsory subjects and open classrooms in order to support students in their self-realization needs and provoke interest in mundane school subjects.

The educational course for pupils «Crystal Growth – from School Desk to Leading Scientific Research» began several years ago with close cooperation between Novosibirsk State University, the Institute of Solid State Chemistry and Mechanochemistry SB RAS and School #162 of Novosibirsk. The aim of the course is to provide further education in Chemistry and Crystallography via laboratory work and lectures, complementing the standard school program. We provide a targeted syllabus for students from 7 to 17 years old, covering related scientific topics starting from crystal symmetry to the basics of physical chemistry. Through close communication and interaction, pupils develop skills in growing crystals, paying particular attention to obtaining large single crystals of different substances. During the course, pupils crystallize more than 15 different substances using at least 5 different methods and their modifications.

At the end of every year, the students are given the opportunity to carry out a personal project, calling on the new knowledge they have obtained from the course. Thus we can assume that an efficient program was developed and realized to support personal ideas and research for school students, based on compulsory subjects and modern experimental techniques.