Hengzongshu/ArticleAgent huggingface dataset:
: The ability of the nervous system to detect a wide range of noxious stimuli is crucial to avoid life-threatening injury and to trigger protective behavioral and physiological responses. Pain represents a complex phenomenon, including nociception associated with cognitive and emotional processing. Animal experimental models have been developed to understand the mechanisms involved in pain response, as well as to discover novel pharmacological and non-pharmacological anti-pain therapies. Due to the genetic tractability, similar physiology, low cost, and rich behavioral repertoire, the zebrafish (Danio rerio) is a powerful aquatic model for modeling pain responses. Here, we summarize the molecular machinery of zebrafish responses to painful stimuli, as well as emphasize how zebrafish-based pain models have been successfully used to understand specific molecular, physiological, and behavioral changes following different algogens and/or noxious stimuli (e.g., acetic acid, formalin, histamine, Complete Freund's Adjuvant, cinnamaldehyde, allyl isothiocyanate, and fin clipping). We also discuss recent advances in zebrafish-based studies and outline the potential advantages and limitations of the existing models to examine the mechanisms underlying pain responses from evolutionary and translational perspectives. Finally, we outline how zebrafish models can represent emergent tools to explore pain behaviors and pain-related mood disorders, as well as to facilitate analgesic therapy screening in translational pain research.
During the COVID-19 pandemic, Small and medium-sized enterprises have been significantly affected. Domestic measures of state support, including tax incentives, have become an important support factor. The corresponding Resolution provided for the expansion of opportunities to support the implementation of regional support measures.

We carried out an initial comparison of the consequences of the implementation of different support models in three similar regions and predicted the impact of the implemented regional support model on the future development of SMEs. The costs of the region for additional support measures, calculated as the lost budget income, in terms of one employee employed in medium and small businesses, turned out to be very insignificant.

We draw a conclusion about the high economic and political efficiency of support measures associated with reducing the tax burden. It can be said that the regions that did not expand the support measures provided for by Resolution № 409 at the expense of the regional package missed the chance to convert small funds in support of the population.
We discuss some aspects of the development of a variational approach to study the dynamics of climatic and ecological systems under intensive actions of natural and anthropogenic origin. The variational principle essentially represents a versatile tool to create a consistent modeling technology based on models of processes coupled with available observational data. The basic entities included in the formulation of the variational principle are models of processes; data and models of observations; target criteria for forecasting; a priori information about all the required elements of the system. We develop a set of mathematical models combined within the framework of the variational principle. They describe the dynamics of the atmosphere and water bodies in conjunction with a thermally and dynamically heterogeneous surface of the Earth; the hydrological cycle, moisture in the atmosphere and the soil; radiation transfer in the system of the atmosphere and the underlying surface; and transport and transformation of various substances in gaseous and aerosol states in the atmosphere. As an example, we demonstrate the results of calculations performed with a set of numerical models adapted to the conditions of a Novosibirsk city agglomeration. The results of scenario calculations on the formation of mesoclimates and quality of the atmosphere for the typical conditions of Siberian cities are presented.
Apoptosis or programmed cell death is important for multicellular organisms to keep cell homeostasis and for the clearance of mutated or infected cells. Apoptosis can be induced by intrinsic or extrinsic stimuli. The first event in extrinsic apoptosis is the formation of the Death-Inducing Signalling Complex (DISC), where the initiator caspases-8 and -10 are fully activated by several proteolytic cleavage steps and induce the caspase cascade leading to apoptotic cell death. Analysing the processing of procaspases-8 and -10 by Western blot is a commonly used method to study the induction of apoptosis by death receptor stimulation. To analyse procaspase-8 and -10 cleavage, cells are stimulated with a death ligand for different time intervals, lysed and subjected to Western blot analysis using anti-caspase-8 and anti-caspase-10 antibodies. This allows monitoring the caspase cleavage products and thereby induction of apoptosis.
Experimental data on the evolution of artificial controlled travelling disturbances in a three-dimensional supersonic boundary layer on a 45° swept wing at Mach number 2.0 is presented.

Artificial disturbances were introduced in the boundary layer using a periodical glow discharge with a frequency of 20 kHz. Pulsations of the boundary layer were measured with a constant-temperature hot-wire anemometer and the probe moved parallel and not parallel to the leading edge of the model. In both cases spatial-temporal and spectral-wave characteristics of the wave train development were obtained and quantitatively compared with each other.

This paper discusses the suitability of the method for measuring fluctuations fields when the probe is moving not parallel to the leading edge of the model.
Transformation of a Bessel beam by a lens results in the formation of a “perfect” vortex beam (PVB) in the focal plane of the lens. The PVB has a single-ring cross-section and carries an orbital angular momentum (OAM) equal to the OAM of the “parent” beam. PVBs have numerous applications based on the assumption of their ideal ring-type structure. For instance, we proposed using terahertz PVBs to excite vortex surface plasmon polaritons propagating along cylindrical conductors and the creation of plasmon multiplex communication lines in the future (Comput. Opt. 2019, 43, 992). Recently, we demonstrated the formation of PVBs in the terahertz range using a Bessel beam produced using a spiral binary silicon axicon (Phys. Rev. A 2017, 96, 023846). It was shown that, in that case, the PVB was not annular, but was split into nested spiral segments, which was obviously a consequence of the method of Bessel beam generation. The search for methods of producing perfect beams with characteristics approaching theoretically possible ones is a topical task. Since for the terahertz range, there are no devices like spatial modulators of light in the visible range, the main method for controlling the mode composition of beams is the use of diffractive optical elements. In this work, we investigated the characteristics of perfect beams, the parent beams being quasi-Bessel beams created by three types of diffractive phase axicons made of high-resistivity silicon: binary, kinoform, and “holographic”. The amplitude-phase distributions of the field in real perfect beams were calculated numerically in the approximation of the scalar diffraction theory. An analytical expression was obtained for the case of the binary axicon. It was shown that a distribution closest to an ideal vortex was obtained using a holographic axicon. The resulting distributions were compared with experimental and theoretical distributions of the evanescent field of a plasmon near the gold–zinc sulfide–air surface at different thicknesses of the dielectric layer, and recommendations for experiments were given.
Hsp67Bc in Drosophila melanogaster is a member of the small heat shock protein family, the main function of which is to prevent the aggregation of misfolded or damaged proteins. Hsp67Bc interacts with Starvin and Hsp23, which are known to be a part of the cold stress response in the fly during the recovery phase.

In this study, we investigated the role of the Hsp67Bc gene in the cold stress response. We showed that in adult Drosophila, Hsp67Bc expression increases after cold stress and decreases after 1.5 h of recovery, indicating the involvement of Hsp67Bc in short-term stress recovery. We also implemented a deletion in the D. melanogaster Hsp67Bc gene using imprecise excision of a P-element, and analysed the cold tolerance of Hsp67Bc-null mutants at different developmental stages.

We found that Hsp67Bc-null homozygous flies are viable and fertile but display varying cold stress tolerance throughout the stages of ontogenesis: the survival after cold stress is slightly impaired in late third instar larvae, unaffected in pupae, and notably affected in adult females. Moreover, the recovery from chill coma is delayed in Hsp67Bc-null adults of both sexes. In addition, the deletion in the Hsp67Bc gene caused more prominent up-regulation of Hsp70 following cold stress, suggesting the involvement of Hsp70 in compensation of the lack of the Hsp67Bc protein. Taken together, our results suggest that Hsp67Bc is involved in the recovery of flies from a comatose state and contributes to the protection of the fruit fly from cold stress.
Данная работа посвящена исследованию закрученных течений. Течения с закруткой потока находят широкое применение в различных технологических процессах. Закрученные течения могут сопровождаться такими нестационарными эффектами, как прецессия вихревого ядра. В свою очередь крупномасштабные пульсации, вызванные прецессией вихря, могут привести к повреждению конструкций и снижению надежности оборудования. Таким образом, для инженерных расчетов требуются подходы, достаточно хорошо описывающие подобные течения. В данной работе представлена методика описания закрученных потоков апробированная в рамках программных комплексов Fluent и SigmaFlow. Проведено численное моделирование нескольких тестовых задач с закруткой потока. Полученные результаты сопоставлены между собой, а также с экспериментальными данными.
We predict a generic mechanism of wave localization at an interface between uniform artificial gauge fields, arising due to propagation-dependent phase accumulation similar to Aharonov-Bohm phenomenon. We realize experimentally a synthetic mesh lattice with real-time control over the vector gauge field, and observe robust localization under a broad variation of gauge strength and direction, as well as structural lattice parameters. This suggests new possibilities for confining and guiding waves in diverse physical systems through the synthetic gauge fields.
Интегральная модель волновых режимов течения тонкого слоя вязкой жидкости

учитывающая члены второго порядка малости
The parasitic flatworm Opisthorchis felineus is one of the causative agents of opisthorchiasis in humans. Recently, we assembled the O. felineus genome, but the correct genome annotation by means of standard methods was hampered by the presence of spliced leader trans-splicing (SLTS). As a result of SLTS, the original 5'-end (outron) of the transcripts is replaced by a short spliced leader sequence donated from a specialized SL RNA. SLTS is involved in the RNA processing of more than half of O. felineus genes, making it hard to determine the structure of outrons and bona fide transcription start sites of the corresponding genes and operons, being based solely on mRNA-seq data.

In the current study, we tested various experimental approaches for identifying the sequences of outrons in O. felineus using massive parallel sequencing. Two of them were developed by us for targeted sequencing of already processed branched outrons. One was based on sequence-specific reverse transcription from the SL intron toward the 5'-end of the Y-branched outron. The other used outron hybridization with an immobilized single-stranded DNA probe complementary to the SL intron. Additionally, two approaches to the sequencing of rRNA-depleted total RNA were used, allowing the identification of a wider range of transcripts compared to mRNAseq. One is based on the enzymatic elimination of overrepresented cDNAs, the other utilizes exonucleolytic degradation of uncapped RNA by Terminator enzyme.

By using the outron-targeting methods, we were not able to obtain the enrichment of RNA preparations by processed outrons, which is most likely indicative of a rapid turnover of these trans-splicing intermediate products. Of the two rRNA depletion methods, a method based on the enzymatic normalization of cDNA (Zymo-Seq RiboFree) showed high efficiency. Compared to mRNA-seq, it provides an approximately twofold increase in the fraction of reads originating from outrons and introns. The results suggest that unprocessed nascent transcripts are the main source of outron sequences in the RNA pool of O. felineus.
Russian scientific schools, as special phenomenon, which formation is a consequence of the cultural and historical development of country, cannot develop in isolation

close interaction between researchers of all levels and ages is necessary

In the conditions of the development of the information society and the emerging knowledge society, not only the involvement of young people in science, but also the interpersonal interaction of young scientists who are representatives of various scientific schools and professional communities is of particular relevance
Self-renewal of cultured pluripotent stem cells is a complex process, which includes multiple functional and regulatory levels. Transcription factors, their target genes, chromatin modifiers, signaling pathways, and regulatory noncoding RNAs are involved in the maintaining of self-renewal. Studies of molecular and genetic bases of maintaining self-renewal and pluripotency in cultured mammalian cells are important to understand processes in preimplantation embryogenesis and to develop efficient techniques to obtain pluripotent stem cell lines for experimental biology and medicine. MicroRNAs (miRNAs) play an important role in pluripotency maintaining and reprogramming. However, involvement of this class of noncoding RNAs and functions of individual molecules are poorly studied. The goal of this study was the search for the miRNAs potentially involved in the pluripotency maintaining and reprogramming of Rattus norvegicus cells. We analyzed the expression of miRNAs in rat embryonic stem cells, induced pluripotent stem cells and embryonic fibroblasts using bioinformatic methods and data obtained with next generation sequencing. The analysis of differential expression between groups of rat pluripotent cells and fibroblasts, and the analysis of experimentally confirmed target genes of differentially expressed known rat miRNAs revealed novel potential players of pluripotency maintaining and reprogramming processes. In addition, novel members of these processes were revealed among novel rat miRNAs. The use of bioinformatic and systems biology approaches is the first step, which is necessary for choosing candidates for the subsequent experimental studies. The results obtained substantially improve our understanding of the self-renewal regulation system of the laboratory rat, a popular biomedical object, and our knowledge about the system in mammals.
Ca,Mg-sulfates are subduction-related sources of oxidized S-rich fluid under lithospheric mantle P,T-parameters.

Experimental study, aimed at the modeling of scenarios of S-rich fluid generation as a result of desulfation and subsequent sulfide formation, was performed using a multi-anvil high-pressure apparatus. Experiments were carried out in the Fe,Ni-olivine–anhydrite–C and Fe,Ni-olivine–Mg-sulfate–C systems (P = 6.3 GPa, T of 1050 and 1450 °C, t = 23–60 h).

At 1050 °C, the interaction in the olivine–anhydrite–C system leads to the formation of olivine + diopside + pyrrhotite assemblage and at 1450 °C leads to the generation of immiscible silicate-oxide and sulfide melts. Desulfation of this system results in the formation of S-rich reduced fluid via the reaction olivine + anhydrite + C → diopside + S + CO2. This fluid is found to be a medium for the recrystallization of olivine, extraction of Fe and Ni, and subsequent crystallization of Fe,Ni-sulfides (i.e., olivine sulfidation). At 1450 °C in the Ca-free system, the generation of carbonate-silicate and Fe,Ni-sulfide melts occurs. Formation of the carbonate component of the melt occurs via the reaction Mg-sulfate + C → magnesite + S. It is experimentally shown that the olivine-sulfate interaction can result in mantle sulfide formation and generation of potential mantle metasomatic agents—S- and CO2-dominated fluids, silicate-oxide melt, or carbonate-silicate melt.
In the course of archaeological research of Russian ostrogs (wooden fortresses) in Siberia, which belong to the end of the 16th – the first half of the 18th centuries, researchers come across remains of defensive fortifications, interpretation of which is rather complicated. Such remains include traces of continuous pile and column foundations of ostrog towers.

In the course of archaeological research, four towers with continuous pile and column foundation were discovered, namely, a south-western tower of Umrevinskii Ostrog, one of Tobolsk towers, a south-eastern tower and a north-western tower of Udinskii Ostrog. Information on discovery of towers with such a foundation in Mangazeya was not confirmed. The existence of such a foundation under the western tower of the Sosnovskii Ostrog remains disputable. One of such towers preserved its original foundation in the town of Bel’sk (Irkutsk region) till 1987. Three towers with continuous pile and column foundations were discovered in Krasnoyarskii Ostrog in written sources.

It is determined that all towers were located on the slope or next to it, their dimensions were considerably smaller than average ones, while their depth and thickness of logs were equal and met the same parameters of the lath fence walls. Such towers were intended first for reducing a load on unstable grounds on the slopes and next to them at the expense of small sizes of towers and secondly for preventing uneven subsidence of the tower bases, which due to a small perimeter thereof can result in a considerable lurch and subsequent collapse or damage of the structural integrity of the building.
Electronic correlation is believed to play an important role in exotic phenomena such as insulator-metal transition, colossal magneto resistance and high temperature superconductivity in correlated electron systems. Recently, it has been shown that electronic correlation may also be responsible for the formation of unconventional plasmons. Herewith, using a combination of angle-dependent spectroscopic ellipsometry, angle resolved photoemission spectroscopy and Hall measurements all as a function of temperature supported by first-principles calculations, the existence of low-loss high-energy correlated plasmons accompanied by spectral weight transfer, a fingerprint of electronic correlation, in topological insulator (Bi$_{0.8}$Sb$_{0.2}$)$_2$Se$_3$ is revealed. Upon cooling, the density of free charge carriers in the surface states decreases whereas those in the bulk states increase, and that the newly-discovered correlated plasmons are key to explaining this phenomenon. Our result shows the importance of electronic correlation in determining new correlated plasmons and opens a new path in engineering plasmonic-based topologically-insulating devices.
The crystal structure of {Ni 3 (H 2 O) 3 (Bpa) 4 }(V 6 O 18 )]•8H 2 O is a 3D framework constructed from three interpenetrated metal organic subnets, corner linked to the vanadium oxide subunit.This connectivity generates a crystal structure that combines both a 3D metal-organic and inorganic frameworks.The metal-organic subnet consists of three interpenetrating
A number of new chiral bispidines containing monoterpenoid fragments have been obtained. The bispidines were studied as ligands for Ni-catalyzed addition of diethylzinc to chalcones.

The conditions for chromatographic analysis by HPLC-UV were developed, in which the peaks of the enantiomers of all synthesized chiral products were separated, which made it possible to determine the enantiomeric excess of the resulting mixture.

It was demonstrated that bispidine-monoterpenoid conjugates can be used as the ligands for diethylzinc addition to chalcone C=C double bond but not as inducers of chirality. Besides products of ethylation, formation of products of formal hydrogenation of the chalcone C=C double bond was observed in all cases. Note, that this formation of hydrogenation products in significant amounts in the presence of such catalytic systems was found for the first time. A tentative scheme explaining the formation of all products was proposed.
In his De communi mathematica scientia Iamblichus famously distinguishes two orders of initiation within the Pythagorean School. Clement of Alexandria as a 'Neopythagorean Philosopher' is relatively badly served. The goal of the Pythagoreans consists not in abstaining from doing certain important things, but rather in abstention from harmful and useless things in order to attain to a better performance in those which are really vital. Thus, Pythagoras discoursed about sciences with those inclined to philosophy, while the rest received ethical maxims in a 'symbolic' manner. Clement, however, does not want to know about any schism: this twofold education is considered by him a well-designed technique, which gradually leads the students to the 'revealed knowledge, reserved for the elite'. His distinction between fundamental belief and the highest faith, on the one hand, and the scientific knowledge and gnosis , on the other, became fundamental for the later Christian theory of knowledge. Keywords:Alexandria; Christian theory of knowledge; Clement; Iamblichus; philosophy; Pythagorean tradition; science
Background Major depressive disorder (MDD) is associated with an increased risk of brain atrophy, aging-related diseases, and mortality.

Methods We performed a mega-analysis by pooling brain measures derived from T1-weighted MRI scans from 29 samples worldwide. Normative brain aging was estimated by predicting chronological age (10-75 years) from 7 subcortical volumes, 34 cortical thickness and 34 surface area, lateral ventricles and total intracranial volume measures separately in 1,147 male and 1,386 female controls from the ENIGMA MDD working group. The learned model parameters were applied to 1,089 male controls and 1,167 depressed males, and 1,326 female controls and 2,044 depressed females to obtain independent unbiased brain-based age predictions. The difference between predicted 'brain age' and chronological age was calculated to indicate brain predicted age difference (brain-PAD).

Findings On average, MDD patients showed a higher brain-PAD of +0.90 (SE 0.21) years (Cohen's d=0.12, 95% CI 0.06-0.17) compared to controls. Relative to controls, first-episode and currently depressed patients showed higher brain-PAD (+1.2 [0.3] years), and the largest effect was observed in those with late-onset depression (+1.7 [0.7] years). In addition, higher brain-PAD was associated with higher self-reported depressive symptomatology (b=0.05, p=0.004). Interpretation This highly powered collaborative effort showed subtle patterns of abnormal structural brain aging in MDD. Substantial within-group variance and overlap between groups were observed. Longitudinal studies of MDD and somatic health outcomes are needed to further assess the predictive value of these brain-PAD estimates.