Hengzongshu/ArticleAgent huggingface dataset:
Bacteriophages are known to be one of the driving forces of bacterial evolution. Besides promoting horizontal transfer of genes between cells, they may induce directional selection of cells (for instance, according to more or less resistance to phage infection). Switching between lysogenic and lytic pathways results in various types of (co)evolution in host-phage systems. Spatial (more generally, ecological) organization of the living environment is another factor affecting evolution.

In this study, we have simulated and analyzed a series of computer models of microbial communities evolving in spatially distributed environments under the pressure of phage infection.We modeled evolving microbial communities living in spatially distributed flowing environments. Non-specific nutrient supplied in the only spatial direction, resulting in its non-uniform distribution in environment. We varied the time and the location of initial phage infestation of cells as well as switched chemotaxis on and off. Simulations were performed with the Haploid evolutionary constructor software ( http://evol-constructor.bionet.nsc.ru/ ).

Simulations have shown that the spatial location of initial phage invasion may lead to different evolutionary scenarios. Phage infection decreases the speciation rate by more than one order as far as intensified selection blocks the origin of novel viable populations/species, which could carve out potential ecological niches. The dependence of speciation rate on the invasion node location varied on the time of invasion. Speciation rate was found to be lower when the phage invaded fully formed community of sedentary cells (at middle and late times) at the species-rich regions. This is especially noticeable in the case of late-time invasion. Our simulation study has shown that phage infection affects evolution of microbial community slowing down speciation and stabilizing the system as a whole. This influencing varied in its efficiency depending on spatially-ecological factors as well as community state at the moment of phage invasion.
A corrigendum on the article The forearm fractures in patients with diabetes and without diabetes in population sample aged over 50 years (Novosibirsk) by E.S. Mazurenko, S.K. Malyutina, L.V. Shcherbakova, S.V. Mustafina, T.M. Nikitenko, M. Bobak, O.D. Rymar (2018). Problems of Endocrinology 65(2). doi: 10.14341/probl9799 There was an error on the page 84 in the Table 4: symbol greater than or equal to was confuse with lower than in the table raw for menopause; symbol lower than or equal to was confuse with greater than in the table raw for total cholesterol level. The authors and editors apologize for this error. The original article has been updated.Ошибка в статье Переломы дистального отдела предплечья у лиц с сахарным диабетом и без нарушений углеводного обмена в популяционной выборке старше 50 лет (Новосибирск) авторского коллектива в составе: Е.С. Мазуренко, С.К. Малютина, Л.В. Щербакова, С.В. Мустафина, Т.М. Никитенко, М. Bobak, О.Д. Рымар, опубликованной в журнале Проблемы эндокринологии 2019;65(2):78-87. DOI: https://doi.org/10.14341/probl9799. На стр. 84 в табл. 4 Результаты логистического регрессионного анализа связи изучаемых параметров с риском переломов ДОП за последние 3 года у женщин Новосибирска были перепутаны символы: знак больше либо равно указан вместо знака меньше в строке Длительность менопаузы; знак меньше либо равно указан вместо знака больше в строке ОХС. Авторы и редакторы сожалеют о допущенной ошибке. Исходная версия статьи была заменена.
For the first time Li2MoO4 crystals, including samples enriched with isotope molybdenum-100 (100Mo), were grown by Czochralski technique at low-thermal-gradient conditions (LTG Cz), which allowed obtaining crystals of record size and quality.

Dependence between growth rate, growth mechanism and crystal faceting was established. Low temperature tests of the produced Li2MoO4 and Li2 100MoO4 scintillating bolometers have demonstrated high performance of the detectors in terms of energy resolution and particle discrimination ability.

Radioactive contamination of the Li2MoO4 and Li2 100MoO4 crystal scintillators produced from selected initial materials after double crystallization was tested in low background underground conditions. The achieved radiopurity level satisfies the requirements of the next generation large scale double beta decay experiments.
It was proved that ethanol is powerful inductor of transpositions MGEs of Drosophila like other different external (temperature shock, y-irradiation) and genetic factors, the main of them are isogenization and selection

Influence of treating with limited doses of steams of ethanol on a transpositions MGE412 was investigated in the males from isogenic lines of Drosophila melanogaster. The rates of induced transpositions was estimated

It was proved the phenomenon of the induction. It was established that alcohol made be finished either death of individuals or increase genetic variability in the posterity
Aim. The goal was to aggregate data on the nesting phenology of the range of Anseriformes in the Baikal Siberia region – using the example of Lake Baikal itself. Material and methods . In preparing the materials we used data from our own observations based on foot and car‐based survey assessments and that of other authors for the entire known period of bird research on Lake Baikal, including data from the literature. Standard ornithological visual methods of accounting, registration and description are used. Results. The ecology of Anseriformes nesting has been sufficiently studied, but information on the oviposition phenology is not generalized. During the nesting periods of Anseriformes on Lake Baikal (in particular) the laying of eggs occurs over an extended time period. This prolongation egg‐laying time is normal and is a response to the death of clutches from inundations as well as being a characteristic of some ducks which re‐nest repeatedly. These subsequently lead to a later taking to flight of young birds. Conclusion. The earliest clutches are found among mallard and ruddy shelduck from the end of April, when ponds are still ice‐bound, while the latest are laid up to midJuly by the European widgeon, the white‐winged scoter and the red‐breasted merganser. Mass oviposition of all species occurs in the period from mid‐May to mid‐June. Overall, the egg‐laying period of Lake Baikal Anseriformes lasts from 20 days to 3.1 months. The timing and period of egg‐laying depends on regional environmental conditions and the specifics of individual species’ ecology and biology. Although the general ovipositional time‐frame of Anseriformes regionally is prolonged, the period of mass egg‐laying is quite concentrated and occurs within a period of 20‐30 days.
The free-living marine flatworm Macrostomum lignano is a powerful model organism for use in studying mechanisms of regeneration and stem cell regulation due to its combination of biological and experimental properties, including the availability of transgenesis methods, which is unique among flatworm models. However, due to its relatively recent introduction in research, many aspects of this animal's biology remain unknown. One such question is the influence of culture temperature on Macrostomum biology.

We systematically investigated how different culture temperatures affect development time, reproduction rate, regeneration, heat shock response, and gene knockdown efficiency by RNA interference (RNAi) in M. lignano. We used marker transgenic lines to accurately measure the regeneration endpoint, and to establish the stress response threshold for temperature shock.

We found that compared to the culture temperature of 20 °C commonly used for M. lignano, temperatures of 25 °C–30 °C substantially increase the speed of development and regeneration, lead to faster manifestation of RNAi phenotypes, and increase reproduction rate without detectable negative consequences for the animal, while temperatures above 30 °C elicit a heat shock response. We show that altering temperature conditions can be used to reduce the time required to establish M. lignano cultures, perform RNAi experiments, store important lines, and optimize microinjection procedures for transgenesis. These findings will help to optimize the design of experiments in M. lignano, and thus facilitate future research using this model organism.
The semiconductor tracker (SCT) is one of the tracking systems for charged particles in the ATLAS detector. It consists of 4088 silicon strip sensor modules. During Run 2 (2015–2018) the Large Hadron Collider delivered an integrated luminosity of 156 fb -1 to the ATLAS experiment at a centre-of-mass proton-proton collision energy of 13 TeV. The instantaneous luminosity and pile-up conditions were far in excess of those assumed in the original design of the SCT detector.

Due to improvements to the data acquisition system, the SCT operated stably throughout Run 2.

It was available for 99.9% of the integrated luminosity and achieved a data-quality efficiency of 99.85%. Detailed studies have been made of the leakage current in SCT modules and the evolution of the full depletion voltage, which are used to study the impact of radiation damage to the modules.
Lateral heterojunctions of atomically precise graphene nanoribbons (GNRs) hold promise for applications in nanotechnology, yet their charge transport and most of the spectroscopic properties have not been investigated.

Here, we synthesize a monolayer of multiple aligned heterojunctions consisting of quasi-metallic and wide-bandgap GNRs, and report characterization by scanning tunneling microscopy, angle-resolved photoemission, Raman spectroscopy, and charge transport. Comprehensive transport measurements as a function of bias and gate voltages, channel length, and temperature

reveal that charge transport is dictated by tunneling through the potential barriers formed by wide-bandgap GNR segments. The current-voltage characteristics are in agreement with calculations of tunneling conductance through asymmetric barriers. We fabricate a GNR heterojunctions based sensor and demonstrate greatly improved sensitivity to adsorbates compared to graphene based sensors. This is achieved via modulation of the GNR heterojunction tunneling barriers by adsorbates.
P-V-T equation of state and thermoelastic properties of shortite Na 2 Ca 2 (CO 3 ) 2 from first principles Theoretical study of structural, thermodynamic and dynamic properties of the double Na-Ca carbonate, shortite, was carried out with density functional theory.Local density approximation (LDA) and generalized gradient approximation in the Perdew-Burke-Ernzerh of (PBE) parameterization were used.Calculated structural and elastic properties exhibit typical behavior for both employed methods.At the same time, equation of state P(V) and bulk modulus, obtained by means of LDA and PBE are significantly different from each other at low pressure and high temperature.This difference is caused by dynamic instability of crystal which boundary calculated using LDA always lies at negative pressure region, while in the case of PBE calculations, this limit manifests itself at positive pressure resulting to features in equation of state P(V) and the bulk modulus behavior.Results indicate that accuracy of used theoretical method is critical for understanding the cause of shortite lattice instability at low pressure and sufficiently high temperature.
Using the ability of poorly differentiated cells to natively internalize fragments of extracellular double-stranded DNA as a marker

we isolated a tumorigenic subpopulation present in Krebs-2 ascites that demonstrated the features of tumor-inducing cancer stem cells. Having combined TAMRA-labeled DNA probe and the power of RNA-seq technology, we identified a set of 168 genes specifically expressed in TAMRA-positive cells (tumor-initiating stem cells), these genes remaining silent in TAMRA-negative cancer cells. TAMRA+ cells displayed gene expression signatures characteristic of both stem cells and cancer cells. The observed expression differences between TAMRA+ and TAMRA− cells were validated by Real Time PCR.

The results obtained corroborated the biological data that TAMRA+ murine Krebs-2 tumor cells are tumor-initiating stem cells. The approach developed can be applied to profile any poorly differentiated cell types that are capable of immanent internalization of double-stranded DNA.
Nonlinear Fourier transform (NFT) (also known in the mathematical and nonlinear science community as the inverse scattering transform [1] ) has recently attracted a great deal of attention in the context of optical transmission in fiber channels [2] , that can be approximated by the nonlinear Schrodinger equation. Within the NFT-based¨ transmission approach, we modulate the parameters of the nonlinear spectrum (NS) and generate the respective information signal in time domain using inverse NFT. Both discrete and continuous parts of NS can be used, here we focus on the continuous spectrum only. Then, the signal is launched into the fiber, and at the receiver we apply direct NFT to the received signal's to retrieve the information encoded in NS. In this work we demonstrate that the high-accuracy computation of the continuous NS can be performed by using artificial neural networks (NN). The NS of a given localized signal q ( t ) containing no solitonic (discrete) components is represented by the continuous complex-valued function r ( ξ ) (the reflection coefficient) of the real spectral parameter ξ , where the latter plays the role of nonlinear frequency. The signals in our work have been specifically pre-selected to ensure that they contain no discrete spectrum. In the time domain, considered (normalized) symbol is given as the sum of independent sub-carriers [3] : $q(t) = \frac{1}{Q}\sum\nolimits_{k = 1}^M {{C_k}} {e^{i{\omega _k}t}}f(t)$ where M is a number of frequency channels, ω k is a carrier frequency of the k -th channel, C k corresponds to the digital data in k -th channel, and T defines the symbol interval; f ( t ) is the return-to-zero carrier support waveform. To assess the quality of NN prediction, we use the following formula for the relative error: $\eta (\xi ) = \frac{{\left\langle {\left| {{r_{predicted{\text{ }}}}(\xi ) - {r_{actual{\text{ }}}}(\xi )} \right|} \right\rangle }}{{\left\langle {\left| {{r_{actual{\text{ }}}}(\xi )} \right|} \right\rangle }}$ , where 〈•〉 denotes the mean over the spectral interval, the "predicted" and "actual" indices refer to the NN-predicted and precomputed values of the reflection coefficient r ( ξ ), respectively.
The literature showed a limited number of cases of facial pain caused by pathology at the level of the craniovertebral junction, however there is no a single case of pain caused by the migration of elements of the shunt systems.

A female patient, 58 years old, applied to the clinic complaining of constant pain with a paroxysmal component in the left part of the face and back of the skull associated with head movements. Other complaints included a headache accompanied by nausea, numbness in the limbs, instability in vertical position, urinary incontinence, abnormal proprioceptive sensitivity in the limbs. Medical history showed that in 2000 the patient underwent Torkildsen procedure (ventriculo-cisternostomy) due to occlusive hydrocephalus after a neurological infection. In 2013, due to shunt dysfunction endoscopic third ventriculostomy was performed with a good clinical effect. Deterioration started in January of 2018. At hospitalization, tomography of the brain showed migration of the distal end of the shunt into spinal matter, syringobulbia at the C 1 vertebra level. No signs of neurovascular conflict of the trifacial nerves were observed. Removal of the ventriculo-cisternal shunt irritating the spinal nucleus of the trifacial nerve was performed.

In the postoperative period, intensity of the paroxysmal component of facial pain decreased. At day 5 after surgery, the patient was discharged in a satisfactory state. For 1 year and 6 months after the surgery, facial pain has been absent. Manifestations of hydrocephalus and spinal disorder (numbness of the limbs, instability, urinary incontinence) persist, but due to the patient’s compensated state, negative result of the tap-test, signs of functional third ventriculostomy, it was decided to abstain from further interventions. This clinical case demonstrated successful treatment of facial pain developed due to a rare cause – migration of a Torkildsen shunt that currently is rarely encountered.
Many types of modern lasers feature nonlinear properties, which makes controlling their operation a challenging engineering problem. In particular, fibre lasers present both high-performance devices that are already used for diverse industrial applications, but also interesting and not yet fully understood nonlinear systems. Fibre laser systems operating at high power often have multiple equilibrium states, and this produces complications with the reproducibility and management of such devices. Self-tuning and feedback-enabled machine learning approaches might define a new era in laser science and technology. The present study is the first to demonstrate experimentally the application of machine learning algorithms for control of the pulsed regimes in an all-normal dispersion, figure-eight fibre laser with two independent amplifying fibre loops. The ability to control the laser operation state by electronically varying two drive currents makes this scheme particularly attractive for implementing machine learning approaches. The self-tuning adjustment of two independent gain levels in the laser cavity enables generation-on-demand pulses with different duration, energy, spectral characteristics and time coherence. We introduce and evaluate the application of several objective functions related to selection of the pulse duration, energy and degree of temporal coherence of the radiation. Our results open up the possibility for new designs of pulsed fibre lasers with robust electronics-managed control.
This paper presents the results of study of titanium–magnesium catalysts often used in polymerization processes, by photoluminescence spectroscopy (PL) in combination with diffuse reflectance infrared Fourier transform spectroscopy (DRIFTS). The interaction of dibutyl phthalate (DBP) with MgCl 2 was studied at DBP added /Mg = 0–1 (mol/mol). The luminescence spectra with excitation at 278 nm and the excitation spectra for main emission bands were recorded. It was shown that DBP adsorbed on magnesium chloride, both in the form of donor–acceptor complexes (D + A – ) and in the form of molecular complexes. At DBP added /Mg <0.15, the formation of D + A – complexes occur predominantly; with an increase in DBP added /Mg, the fraction of molecular complexes increases. Molecular complexes are destroyed during the treatment of the support by TiCl 4 . In this case, the structure of magnesium chloride is disordered and new coordination–unsaturated sites are formed. This work is a first attempt to apply PL spectroscopy in combination with DRIFTS spectroscopy to study titanium–magnesium Ziegler–Natta catalysts. The application of PL spectroscopy to such systems made it possible to detect interactions within and between donor molecules, which would be particularly challenging to achieve using other spectroscopic methods. Both spectroscopic methods provided crucial information about the existence of two types of complexes on the sample surface which is important for tuning the synthesis procedure of the titanium–magnesium catalysts for olefin polymerization.
Previously, it was shown that the HFE gene (associated with human hereditary hemochromatosis) has several haplotypes of intronic polymorphisms. Some haplotype frequencies are race specific and hence can be used in phylogenetic analysis.

We assumed that analysis of Caucasoid patients-living now in Western Siberia and having diseases associated with dietary habits and metabolic rate-will allow us to understand the processes of possible selection during settling of the northern part of Asia.Haplotype analysis of Northern Eurasian native and recently settled ethnic groups was performed on polymorphisms rs1799945, rs1800730, rs1800562, rs2071303, rs1800708, rs1572982, rs2794719, rs807209, and rs2032451 of this gene. Haplotype analysis for the rs1799945, rs1800730, rs1800562, rs2071303, rs1800708, and rs1572982 was performed on Russian patients with some metabolic disorders or stomach cancer and among long-lived people.

The CCA haplotype of the rs2071303, rs1800708, and rs1572982 was found to be associated with HLA-A2 (39 %) in Asian populations. Decreased frequencies of the TTA haplotype (T in rs2071303, T in rs1800708, and A in rs1572982) were observed in the groups of patients with diseases associated with overweight (fatty liver disease, type 2 diabetes mellitus, or metabolic syndrome + arterial hypertension) as compared with the control sample. We detected significant differences in this haplotype's frequency between the patients with type 2 diabetes mellitus and Russian adolescents, elderly citizens, and long-lived people (χ(2) P value = 0.003, 0.010, and 0.015, respectively).No significant differences in frequencies of the alleles with mutations in coding regions of the HFE gene (C282Y, H63D, and S65C) were detected between the analyzed patients (with stomach cancer, metabolic syndrome, fatty liver disease, or type 2 diabetes mellitus) and the control Caucasoid sample. Monophyletic origin of H63D (rs1799945) was confirmed in Caucasoids and Northern Asians. The reasons for a sharp increase in the frequency of CCA haplotype of HFE in the Asian race remain unclear.
Human apurinic/apyrimidinic (AP) endonuclease APE1 catalyses the hydrolysis of phosphodiester bonds on the 5' side of an AP-site (in the base excision repair pathway) and of some damaged nucleotides (in the nucleotide incision repair pathway). The range of substrate specificity includes structurally unrelated damaged nucleotides.

Here, to examine the mechanism of broad substrate specificity of APE1, we performed pulsed electron-electron double resonance (PELDOR) spectroscopy and pre-steady-state kinetic analysis with Förster resonance energy transfer (FRET) detection of DNA conformational changes during DNA binding and lesion recognition. Equilibrium PELDOR and kinetic FRET data revealed that DNA binding by APE1 leads to noticeable damage-dependent bending of a DNA duplex. Molecular dynamics simulations showed that the damaged nucleotide is everted from the DNA helix and placed into the enzyme's binding pocket, which is formed by Asn-174, Asn-212, Asn-229, Ala-230, Phe-266 and Trp-280.

Nevertheless, no damage-specific contacts were detected between these amino acid residues in the active site of the enzyme and model damaged substrates containing 1,N6-ethenoadenosine, α-adenosine, 5,6-dihydrouridine or F-site. These data suggest that the substrate specificity of APE1 is controlled by the ability of a damaged nucleotide to flip out from the DNA duplex in response to an enzyme-induced DNA distortion.