Hengzongshu/ArticleAgent huggingface dataset:
A strategy for improving export and import of Russian regions requires the identification of macroeconomic factors affecting their foreign trade. According to the scientific principle of deduction (from general to particular), trends observed in highly aggregated objects should be assessed first. Time series for foreign trade of the Siberian Federal District were analysed and compared with those of the Russian Federation, Central Federal District and the city of Moscow. Methods of comparative, structural, expert analysis, statistical estimation and econometric modelling are utilised in the research. The study demonstrates that the separation of profit centres and production sites through legal registration of company head offices (the mechanism called «administrative resource») negatively affects regional exports, as well as other aspects. 2018–2019 ratings of Russian regions in terms of production, volume of operations and gross regional product (GRP) per capita revealed the centre’s monopoly in the mining and manufacturing sectors, causing further centralisation of regional budgetary resources. Application of the method of stepwise regression based on partial correlations demonstrated the significant influence of world oil prices and the weak impact of sanctions for Russian trade. It was statistically confirmed that trade in the Siberian Federal District primarily depends on world metal prices, and then the rouble exchange rate, while the influence of the price of oil, whose export was practically taken out of the region, is quite weak. The proposed combination of profit centres with places of its generation is considered as an effective tool for regional equalisation of the foreign trade effect, aimed at overcoming the socio-economic heterogeneity of regions without involving the federal budget. The obtained results can be used by government bodies and research centres for developing regional strategies.
The conventional Friedmann cosmology is known to be in tension with the existence of stable particles having interaction strength typical for supersymmetry and heavier than several TeV. A possible way to save life of such particles may be a modification of the standard cosmological expansion law in such a way that the density of these heavy relics would be significantly reduced. We study particle creation in the Starobinsky inflationary model for different decay channels of the scalaron. It is shown that in the process of thermalization superheavy stable particles with the coupling strength typical for the GUT SUSY could be created with the density equal to the observed density of dark matter.
The charts of the apparent diffusion coefficient (ADC) of patients with histologically confirmed tumors of the brain and spinal canal were analyzed according to the data of magnetic resonance imaging studies performed before surgical treatment. Analysis of the data revealed that the indexes on the ADC-charts largely correlate with the activity of tumor cell proliferation. The signal on the ADC-charts in malignant tumors of grade 3 and 4 is significantly lower than in tumors with low malignancy of 1 and 2 grade. Analysis of ADC-charts in patients with pathological lesions in the central nervous system can be used not only for differential diagnosis, but also embodied in the supposed pathomorphological characteristics of the lesion, thereby influencing the planning of the volume of surgical intervention and treatment options.
When modeling flows of an incompressible fluid, it is convenient sometimes to use the method of discrete vortices (MDV), where the continuous vorticity field is approximated by a set of discrete vortex elements moving in the velocity field. The vortex elements have a clear physical interpretation, they do not require the construction of grids and are automatically adaptive, since they concentrate in the regions of greatest interest and successfully describe the flows of a non-viscous fluid. The possibility of using MDV in simulating flows of a viscous fluid was considered in the previous papers using the examples of flows past bodies with sharp edges with the no-penetration condition at solid boundaries. However, the appearance of vorticity on smooth boundaries requires the no-slip condition to be met when MDV is realized, which substantially complicates the initially simple method. In this connection, an approach is considered that allows solving the problem by simple means.
Cervical cancer screening is based on cytologic analysis and high-risk human papillomavirus (HR-HPV) testing, each having their drawbacks. Implementation of new biomarker-based methods may improve screening accuracy.

Here, the levels of 25 microRNAs (miRNAs, miRs) and 12 mRNAs involved in cervical carcinogenesis in 327 air-dried Papanicolaou-stained cervical smears from patients with cervical precancerous lesions, cancer, or without the disease were estimated by real-time PCR. Using logistic regression analysis, small-scale miRNA-based, mRNA-based, and combined molecular classifiers were built based on paired ratios of miRNA or mRNA concentrations; their ability to detect high-grade cervical lesions and cancer was then compared. Additionally, in a subsample of the same specimens, the levels of MIR124-2 and MAL promoter methylation, HR-HPV genotypes, and viral loads were analyzed.

Combined mRNA-miRNA classifiers manifested a better combination of sensitivity and specificity than miRNA- and mRNA-based classifiers. The best classifier, combining miR-375, miR-20, miR-96, CDKN2A, TSP4, and ECM1, predicted high-grade lesions with diagnostic sensitivity of 89.0%, specificity of 84.2%, and a receiver-operating characteristic area under the curve of 0.913. The relative high-grade lesion risk estimated by the classifier correlated with the frequency of MAL and MIR124-2 methylation but not with the HR-HPV genotype or viral load. The results support the feasibility of cellular biomarker-based methods for cervical screening and patient management.
The prevention of muscle atrophy carries with it clinical significance for the control of increased morbidity and mortality following physical inactivity. While major transcriptional events associated with muscle atrophy-recovery processes are the subject of active research on the gene level, the contribution of non-coding regulatory elements and alternative promoter usage is a major source for both the production of alternative protein products and new insights into the activity of transcription factors.

We used the cap-analysis of gene expression (CAGE) to create a genome-wide atlas of promoter-level transcription in fast (m. EDL) and slow (m. soleus) muscles in rats that were subjected to hindlimb unloading and subsequent recovery.

We found that the genetic regulation of the atrophy-recovery cycle in two types of muscle is mediated by different pathways, including a unique set of non-coding transcribed regulatory elements. We showed that the activation of 'shadow' enhancers is tightly linked to specific stages of atrophy and recovery dynamics, with the largest number of specific regulatory elements being transcriptionally active in the muscles on the first day of recovery after a week of disuse. The developed comprehensive database of transcription of regulatory elements will further stimulate research on the gene regulation of muscle homeostasis in mammals.
ASPD is a new curated database that incorporates data on full-length proteins, protein domains and peptides that were obtained through in vitro directed evolution processes (mainly by means of phage display). At present, the ASPD database contains data on 195 selection experiments, which were described in 112 original papers. For each experiment, the following information is given: (i) description of the target for binding, (ii) description of the protein or peptide which serves as the template for library construction and description of the native protein which binds the target, (iii) links to the major proteomic databases (SWISS-PROT, PDB, PROSITE and ENZYME), (iv) keywords referring to the biological significance of the experiment, (v) aligned sequences of proteins or peptides retrieved through in vitro evolution and relevant native or constructed sequences, (vi) the number of rounds of selection/amplification and (vii) the number of occurrences of clones with each sequence. The literature data include a full reference, a link to the MEDLINE database and the name of the corresponding author with his email address. ASPD has a user-friendly interface which allows for simple queries using the names of proteins and ligands, as well as keywords describing the biological role of the interaction studied, and also for queries based on authors' names. It is also possible to access the database by means of the SRS system, allowing complex queries. There is a BLAST search tool against the ASPD for looking directly for homologous sequences. Research tools of the ASPD allow the analysis of pairwise correlations in the sequences of proteins and peptides selected against one target. The URL for the ASPD database is http://www.sgi.sscc.ru/mgs/gnw/aspd/.
Chromosomes are hierarchically folded within cell nuclei into territories, domains and subdomains, but the functional importance and evolutionary dynamics of these hierarchies are poorly defined.

Here, we comprehensively profile genome organizations of five Anopheles mosquito species and show how different levels of chromatin architecture influence each other. Patterns observed on Hi-C maps are associated with known cytological structures, epigenetic profiles, and gene expression levels. Evolutionary analysis reveals conservation of chromatin architecture within synteny blocks for tens of millions of years and enrichment of synteny breakpoints in regions with increased genomic insulation. However, in-depth analysis shows a confounding effect of gene density on both insulation and distribution of synteny breakpoints.

suggesting limited causal relationship between breakpoints and regions with increased genomic insulation. At the level of individual loci, we identify specific, extremely long-ranged looping interactions, conserved for ~100 million years. We demonstrate that the mechanisms underlying these looping contacts differ from previously described Polycomb-dependent interactions and clustering of active chromatin.
The article presents the results of studying the geological structure of the Bazhenov Formation in the Nadym-Ob interfluve of Western Siberia with the aim of predicting the oil content of this black shale stratum. As a result of interpretation of a wide range of well logging represented by electric, radioactive and acoustic logging, with subsequent matching of these results with paleontological definitions of micro- and macrofauna, the distribution of the Salym, Nizhnevartovsky and Tarkosalinsky types of sections of the Bazhenov Formation was clarified, transitional areas between them were identified. It has been established that the Tarkosalinsky type is more widespread in the western direction than was shown earlier and is also distinguished in the Vengayakhinskaya, Yaraynerskaya and other areas. The Nizhnevartovsky type, on the contrary, has a narrower distribution and stands out directly within the same name arc and to the south by the Variegasky-Tagrinsky megahigh. On the basis of geological, geochemical, geophysical criteria and the results of an inflow test in deep wells, a map of the oil potential prospects of the “classical” sections of the Bazhenov Formation has been compiled. Regional prerequisites (high catagenesis of organic matter, significant modern concentrations of organic carbon, etc.) for the discovery of industrial accumulations of oil in the Bazhenov Formation in the southern regions of the Yamalo-Nenets Autonomous Area are identified. The results of the test for the inflow of the Bazhenov Formation in this area in the 70–90s XX century were analyzed; repeated, interval testing of these deposits using modern methods of stimulation of the inflow is recommended. The necessity of laboratory lithological, petrophysical, geochemical study of the core of the Bazhenov Formation in the southern part of the Yamalo-Nenets Autonomous Area is substantiated with the aim of determining its lithological composition, identifying oil source and oil productive intervals, studying the reservoir structure and the nature of saturation of its void space, developing recommendations for calculating oil reserves and creating technology for its cost-effective production.
It is known that the metabolic syndrome (MS), which includes hypertension, dislipidemia, glucose intolerance, and obesity leads to cardiovascular diseases. The MS risk is growing catastrophically. Molecular mechanisms allowing to understand the reason of integrated dysfunctions, taking place at MS cases, have remained almost unstudied. The chronical stress plays a crucial role in MS development; therefore in the present work a hypertensive rat strain with Inherited Stress-Induced Arterial Hypertension (ISIAH) was used as a model. It was shown that ISIAH rat strain as compared with the control WAG rat strain is characterized by increased content of triglyceride, VLDL and LDL cholesterols, a decreased content of HDL cholesterol, a high level of apolipoprotein B-100, and decreased level of apolipoprotein А-I. The ISIAH rats body weight was higher as compared with WAG rats; ISIAH rats blood glucose content was higher too. Thus, strain hypertension for ISIAH rat is accompanied by dislipidemia, increased glucose content, and increased body weight, representing a whole set of MS signs. Since at MS cases the systemic abnormalities in lipid and carbohydrate metabolism take place, the functional activity of transcription factors (TFs) participating in integral regulation of lipid and carbohydrate metabolism genes in liver was measured. PPAR, LXR, PXR, CAR DNA-binding activity was increased in ISIAH rats, suggesting involvement of these TFs in MS development. Integrated investigation of PPAR, LXR, PXR, CAR regulatory mechanisms, signal transduction and transcriptional targets will provide insights into the pathogenesis of MS and offer valuable information for designing of drugs for MS treatment.
C‐, N‐, P‐, and S‐nucleophiles reacted with symmetrical tris(2,3,5,6‐tetrathiaaryl)methyl cations, generated from the corresponding triarylmethanols by strong acids, to give a variety of asymmetrical monosubstituted persistent triarylmethyl (TAM) radicals as the major products. The only byproducts were symmetrical TAMs.
We discuss some aspects of the development of a variational approach to study the dynamics of climatic and ecological systems under intensive actions of natural and anthropogenic origin. The variational principle essentially represents a versatile tool to create a consistent modeling technology based on models of processes coupled with available observational data. The basic entities included in the formulation of the variational principle are models of processes; data and models of observations; target criteria for forecasting; a priori information about all the required elements of the system.

We develop a set of mathematical models combined within the framework of the variational principle. They describe the dynamics of the atmosphere and water bodies in conjunction with a thermally and dynamically heterogeneous surface of the Earth; the hydrological cycle, moisture in the atmosphere and the soil; radiation transfer in the system of the atmosphere and the underlying surface; and transport and transformation of various substances in gaseous and aerosol states in the atmosphere.

As an example, we demonstrate the results of calculations performed with a set of numerical models adapted to the conditions of a Novosibirsk city agglomeration. The results of scenario calculations on the formation of mesoclimates and quality of the atmosphere for the typical conditions of Siberian cities are presented.
The objects of the study were recombinant clones of Komagataella phaffii K51 carrying the heterologous proteinase K (PK-w) gene from Tritirachium album integrated into their genome as well as samples of recombinant proteinase K isolated from these clones. The aims of this work were i) to determine whether it is possible to create recombinant K. phaffii K51 clones overexpressing functionally active proteinase K from T. album and ii) to analyze the enzymatic activity of the resulting recombinant enzyme.

The following methods were used: computational analysis of primary structure of the proteinase K gene, molecular biological methods (PCR, electrophoresis of DNA in an agarose gel, electrophoresis of proteins in an SDS polyacrylamide gel under denaturing conditions, spectrophotometry, and quantitative assays of protease activity), and genetic engineering techniques (cloning and selection of genes in bacterial cells Escherichia coli TOP10 and in the methylotrophic yeast K. phaffii K51). The gene encoding natural proteinase K (PK-w) was designed and optimized for expression in K. phaffii K51. The proteinase K gene was synthesized and cloned within the plasmid pPICZα-A vector in E. coli TOP10 cells. The proteinase K gene was inserted into pPICZα-A in such a way that - at a subsequent stage of transfection into yeast cells - it was efficiently expressed under the control of the promoter and terminator of the AOX1 gene, and the product of the exogenous gene contained the signal peptide of the Saccharomyces cerevisiae a-factor to ensure the protein's secretion into the culture medium. The resultant recombinant plasmid (pPICZα-A/PK-w) was transfected into K. phaffii K51 cells.

A recombinant K. phaffii K51 clone was obtained that carried the synthetic proteinase K gene and ensured its effective expression and secretion into the culture medium. An approximate productivity of the yeast recombinant clones for recombinant proteinase K was 25 μg/ mL after 4 days of cultivation. The resulting recombinant protease has a high specific proteolytic activity: ~5000 U/mg.
We perform linear stability analysis of Landau jet using quasi-parallel approximation and self-similar approach. While the former detects only sinusoidal unstable modes, the latter also captures axisymmetric disturbances in agreement with previous results. The direct comparison of dispersion curves and eigenmode shapes for m = 1 and Re D ≈ 56 indicates that low frequencies are poorly described within quasi-parallel approximation making this approach questionable for a laminar-turbulent transition model.
We perform Large-eddy simulations of an annular swirling turbulent jet for S = 0, S = 0.3 and S = 1, where S is swirl number. The Reynolds number based on the bulk velocity in the feeding channel and the outer diameter was 8900.

The analysis showed that the region around the stagnation point in the end of the recirculation zone has a high level of turbulent kinetic energy. The length of the recirculation zone decreases in case for low swirl (S = 0.3) compared to the non-rotating case, while for the higher rotation rate (S = 1) the recirculation bubble becomes enormously long.

Spectral analysis showed two peaks corresponding to the Kelvin-Helmholtz instability and some low-frequency oscillations of the recirculating zone.
OBJECTIVE: Agouti protein (AP) and agouti-related protein with a similar sequence and action are endogenous antagonists of melanocortin receptors, implicated in the control of the hypothalamo-pituitary-adrenal (HPA) axis. Dominant mutation of the agouti gene (agouti yellow (A(y))) in heterozygous A(y)/a mice leads to ectopic overexpression of AP and produces an obese phenotype. The existing data on the HPA function in A(y)/a-mice are equivocal; therefore, the present study aimed to assess HPA function in 3-month-old male C57Bl/6J mice of two agouti genotypes: A(y)/a (ectopic AP overexpression) and a/a (absence of AP). DESIGN AND METHODS: In order to evaluate the HPA function, activating (15-min restriction, ACTH-induced corticosterone production in vitro) and inhibiting (i.p. injection of dexamethasone, 0.02 microg/g body weight) stimuli were employed. To estimate the effect of obesity on some HPA functions, A(y)/a males were subdivided into obese and non-obese groups. RESULTS: Basal plasma concentrations of ACTH and corticosterone; basal corticosterone production in vitro; and feedback inhibition of resting corticosterone levels by dexamethasone were similar in A(y)/a- and a/a-mice. Restraint-induced plasma corticosterone was greater in obese and non-obese A(y)/a-mice than in a/a-mice, whereas restraint-induced plasma ACTH levels were similar. Adrenal cell responses to ACTH (10(-13)-10(-10) M) were higher in obese and non-obese A(y)/a-mice than in a/a-mice. Dexamethasone, injected 3 h prior to stress, inhibited stress-induced corticosterone levels by a significantly greater amount in A(y)/a-mice than in a/a-mice. CONCLUSIONS: AP may have both stimulating and inhibiting influences on the HPA axis. AP overproduction increased the response of the HPA to short-restraint stress due to increased adrenal responsiveness to ACTH; this result was not effected by obesity development.
It is demonstrated that the performance of the self-modulated proton driver plasma wakefield accelerator (SM-PDPWA) is strongly affected by the reduced phase velocity of the plasma wave. Using analytical theory and particle-in-cell simulations, we show that the reduction is largest during the linear stage of self-modulation. As the instability nonlinearly saturates, the phase velocity approaches that of the driver. The deleterious effects of the wake's dynamics on the maximum energy gain of accelerated electrons can be avoided using side-injections of electrons, or by controlling the wake's phase velocity by smooth plasma density gradients.
In Eastern Transbaikalia the gold–bismuth mineralization occurs in several large gold deposits (Darasun, Sredne-Golgotayskoe, Umunskoe, etc.). Its age in relation to magmatism has not been constrained yet. This study explores in details the mineralogical and geochemical characteristics of gold–bismuth mineralization in comparison with the other deposits in Transbaikalia. Analysis of geology, as well as geochemical and isotopic studies show that gold–bismuth mineralization can be spatially and genetically linked with the formation of magmatic rocks of the Porphyry Complex of Late Jurassic age.
A graph is called Hamiltonian connected graph if for every pair of distinct vertices , ∈ () there exists a hamiltonian (, )-path in .In this paper we prove Hamiltonian connectivity of the family of infinite two-dimensional diagonal grid induced subgraphs with added horizontal and vertical border edges.A generalization for multidimensional case is given.These results are applied to prove the existence of discrete dynamic systems with arbitrary control functions with some given functioning properties.
A quantum mechanical model of two interacting electrons in graphene is considered. We concentrate on the case of zero total momentum of the pair.

Both stationary and time-dependent problems are considered. It is shown that the complete set of the wave functions with definite energy includes the new functions, previously overlooked. The time evolution of the wave packet, corresponding to the scattering problem setup, leads to the appearance of the localized state at large time.

The asymptotics of this state is found analytically. We obtain the lower bound of the life time of this state, which is connected with the breakdown of the continuous model on the lattice scale. The estimate of this bound gives one a hope to observe the localized states in the experiment.