Hengzongshu/ArticleAgent huggingface dataset:
MicroRNAs (miRNAs) are promising biomarkers in cancer research. Quantitative PCR (qPCR), also known as real-time PCR, is the most frequently used technique for measuring miRNA expression levels. The use of this technique, however, requires that expression data be normalized against reference genes. The problem is that a universal internal control for quantitative analysis of miRNA expression by qPCR has yet to be known.

The aim of this work was to find the miRNAs with stable expression in the thyroid gland, brain and bone marrow according to NanoString nCounter miRNA quantification data.

As a results, the most stably expressed miRNAs were as follows: miR-361-3p, -151a-3p and -29b-3p in the thyroid gland; miR-15a-5p, -194-5p and -532-5p in the brain; miR-140-5p, -148b-3p and -362-5p in bone marrow; and miR-423-5p, -28-5p and -532-5p, no matter what tissue type. These miRNAs represent promising reference genes for miRNA quantification by qPCR.
Magnetic resonance imaging of [1-13 C]hyperpolarized carboxylates (most notably, [1-13 C]pyruvate) allows one to visualize abnormal metabolism in tumors and other pathologies.

Herein, we investigate the efficiency of 1 H and 13 C hyperpolarization of acetate and pyruvate esters with ethyl, propyl and allyl alcoholic moieties using heterogeneous hydrogenation of corresponding vinyl, allyl and propargyl precursors in isotopically unlabeled and 1-13 C-enriched forms with parahydrogen over Rh/TiO2 catalysts in methanol-d4 and in D2 O.

The maximum obtained 1 H polarization was 0.6±0.2 % (for propyl acetate in CD3 OD), while the highest 13 C polarization was 0.10±0.03 % (for ethyl acetate in CD3 OD). Hyperpolarization of acetate esters surpassed that of pyruvates, while esters with a triple carbon-carbon bond in unsaturated alcoholic moiety were less efficient as parahydrogen-induced polarization precursors than esters with a double bond. Among the compounds studied, the maximum 1 H and 13 C NMR signal intensities were observed for propyl acetate. Ethyl acetate yielded slightly less intense NMR signals which were dramatically greater than those of other esters under study.
Drosophila melanogaster and our own species share (Homo sapiens) the history of relatively rapid out-of-Africa dispersal. In Eurasia, they had faced a novel adaptive problem of adjustment of their circadian rhythmicity and night sleep episode to seasonal variation in day length and air temperature. Both species usually respond to heat and a short duration of night by reduction of the amount of night sleep and prolongation of "siesta". To further explore similarities between the two species in the ways of adjustment of their sleep-wake behavior to extreme environmental factors, this study examined the possibility to distinguish four extreme chronotypes among fruit flies and the possibility of the differential response of such chronotypes to light and heat stressors.Circadian rhythms of locomotor activity and sleep-wake pattern were tested in constant darkness, and four strains of fruit flies originating from three wild populations of Africa, Europe, and the USA were selected to represent four distinct chronotypes: "larks" (early morning and evening activity peaks), "owls" (late morning and evening peaks), "swifts" (early morning and late evening peaks), and "woodcocks" (late morning and early evening peaks). The circadian rhythms and sleep efficiency of the selected chronotypes were further tested under such extreme conditions as either long day (LD20:4 at 20°C) or a combination of LD20:4 with hot temperature (29°C).Despite the identity of such experimental conditions for four chronotypes, their circadian rhythms and sleep timing showed significantly distinct patterns of response to exposure to heat and/or long days. All two-way repeated measures analysis of variances yielded a significant interaction between chronotype and time of the day (P<0.001).An experimental study of heritable chronotypes in the fruit fly can facilitate a search for genetic underpinnings of individual variation in vulnerability to circadian misalignment, maladaptive sleep-wake behavior, and sleep disorders.
Creation of a transonic region by means of gas fuel burning in a supersonic flow with a Mach number M = 2 in a channel with a variable cross section is studied numerically. The averaged Navier – Stokes equations closed by the k-ε model of turbulence are solved. The transonic region is created by using gas-dynamic pulses of a throttling jet, which initiates gaseous fuel combustion in the channel region with a constant cross section. The fuel (ethylene or kerosene) is supplied axially into the axisymmetric channel. It is shown that initiation of kerosene combustion requires using gas-dynamic pulses of higher power than those in the ethylene case. The evolution of the kerosene combustion process in channels of different lengths is studied. It is shown that the pulse action duration required for initiation increases with the length of the channel region with a constant cross section.
Heat exchangers used in heating systems usually operate under conditions of variable flow rates and temperatures of heat carriers. However, the theory for calculating the variable modes of operation of such devices is based on the use of constant parameters of heat exchangers. At the same time it is clear that the parameter of the heat exchanger can be affected by all quantities that determine the change of the heat transfer coefficient. In addition, the groups of heat exchangers with a tied supply of heat, i.e. with redistribution of the heat flow in a variable mode of operation, are used.

A new method for calculating the variable modes of operation of heat points with a tied heat supply has been developed. The method of mathematical modeling defines the dependencies that determine the change in the heat exchanger parameters in variable operating modes.

The obtained expressions can be used to configure programmable regulators which will allow the correction of inefficient modes of operation of the heating system.
The paper defined the laser-induced damage threshold from the fluence and the peak power of GaSe and GaSe:In single crystals upon exposure to nanosecond radiation in the two micron range and assessed the influence of test radiation energy parameters (pulse repetition rate, pulse duration) on the damage threshold.

Laser-induced damage threshold was determined with the parameters of the incident radiation close to the pump radiation parameters of promising dual-wavelength optical parametric oscillators (effective pump sources for THz difference frequency oscillators): wavelength was ≈2.1 μm; pulse repetition rates were 10, 12, 14, and 20 kHz; and pulse durations were 15, 18, 20, and 22 ns.

The obtained results made it possible to conclude that the value of GaSe damage threshold at a wavelength of 2.091 μm of the incident radiation was influenced by the accumulation effects (the damage threshold decreased as the pulse repetition rate increased). The accumulation effects were more significant in the case of the In-doped sample, since a more significant decrease in the damage threshold was observed with increasing frequency in terms of the peak power and the fluence.
Whole-chromosome fusions play a major role in the karyotypic evolution of reptiles. It has been suggested that certain chromosomes tend to fuse with sex chromosomes more frequently than others. However, the comparative genomic synteny data are too scarce to draw strong conclusions. We obtained and sequenced chromosome-specific DNA pools of Sceloporus malachiticus , an iguanian species which has experienced many chromosome fusions. We found that four of seven lineage-specific fusions involved sex chromosomes, and that certain syntenic blocks which constitute the sex chromosomes, such as the homologues of the Anolis carolinensis chromosomes 11 and 16, are repeatedly involved in sex chromosome formation in different squamate species. To test the hypothesis that the karyotypic shift could be associated with changes in recombination patterns, we performed a synaptonemal complex analysis in this species and in Sceloporus variabilis (2 n = 34). It revealed that the sex chromosomes in S. malachiticus had two distal pseudoautosomal regions and a medial differentiated region. We found that multiple fusions little affected the recombination rate in S. malachiticus . Our data confirm more frequent involvement of certain chromosomes in sex chromosome formation, but do not reveal a connection between the gonosome–autosome fusions and the evolution of recombination rate. This article is part of the theme issue ‘Challenging the paradigm in sex chromosome evolution: empirical and theoretical insights with a focus on vertebrates (Part II)’.
In this article we present the algorithms of the automated analysis of metrical, strophic and concordance characteristics of Russian poetic texts, realized in the form of processing software of the poetic texts, which can be an important learning tool for philologists in comprehensive analysis of the poems. The results of the such of analysis will allow to expand essentially the possibilities of the philologists who study as the listed levels of verses, as their semantic and pragmatic characteristics, and also to free the philologists from routine work, to expand the range of analyzed works by reducing the dependence of the quality of the comparative analysis on the personal knowledge of the researcher, and also to apply the different methods of intellectual analysis of data.
A human exhaled air analysis by means of infrared (IR) laser photoacoustic spectroscopy is presented.

Eleven healthy nonsmoking volunteers (control group) and seven patients with chronic obstructive pulmonary disease (COPD, target group) were involved in the study. The principal component analysis method was used to select the most informative ranges of the absorption spectra of patients' exhaled air in terms of the separation of the studied groups.

It is shown that the data of the profiles of exhaled air absorption spectrum in the informative ranges allow identifying COPD patients in comparison to the control group.
The Drosophila S2 tissue culture cells are a widely used system for studies on mitosis. S2 cells are particularly sensitive to gene silencing by RNA interference (RNAi), allowing targeted inactivation of mitotic genes. S2 cells are also well suited for high-resolution light microscopy analysis of mitosis in fixed cells, and can be easily immunostained to detect mitotic components. In addition, S2 cells are amenable to transformation with plasmid encoding fluorescently tagged mitotic proteins, allowing in vivo analysis of their behavior throughout cell division. However, S2 cells have not been widely used for transmission electron microscopy (TEM) analysis, which provides ultrastructural details on the morphology of the mitotic apparatus that cannot be obtained with high-resolution confocal microscopy.

Here, we describe a simple method for the ultrastructural analysis of mitosis in Drosophila S2 cells. Our method, which involves fixation and sectioning of a cell pellet, provides excellent preservation of mitotic structures and allows analysis of a higher number of mitotic divisions per sample, compared to correlative light-electron microscopy.

Dividing cells are randomly oriented within the pellet and are sectioned along different planes, providing all-around information on the structure of the mitotic apparatus.
Presented paper is the next step in the research of axial transport in Gas Dynamic Trap. Experiments dedicated to the neutral gas role in the expander of mirror device were carried out. Ion current density distribution measured at the end plate does not depend on neutral gas density in the expander. Experimental indications of neutral gas extrusion from the axis of the expander to its periphery were observed. Numerical model describing such extrusion by elastic collisions of neutrals with plasma ions is in agreement with experimental data.
Proceeds in the form of a mineral extraction tax are one of the main items of income for the federal budget of Russia. Due to the use of preferential ratios in calculating the tax rate of the mineral extraction tax on oil, it can be said that there are tax amounts that were not received by the budget due to the use of tax incentives.

As part of this study, the actual and real tax burden of the oil-producing regions of Russia was calculated and analyzed. The applied method of clustering of oil-producing regions allowed us to identify clusters depending on the volume of oil production and the real average tax burden on mineral extraction tax in the form of oil.

The results showed that the mineral extraction tax in the form of oil is not characterized by a direct relationship between the tax base and the tax rate.
Opisthorchiasis caused by food-borne trematode Opisthorchis felineus is a substantial public health problem, with 17 million persons infected worldwide. This chronic disease is associated with hepatobiliary inflammation, cholangiocyte dysplasia, cholangiofibrosis, intraepithelial neoplasia, and even cholangiocarcinoma among chronically infected individuals.

To provide first insights into the mechanism by which O. felineus infection causes precancerous liver lesions, we investigated the level of oxidative stress (lipid peroxidation byproducts and 8-hydroxy-2′-deoxyguanosine) as well as the time course profiles of chronic inflammation and fibrogenesis markers in the dynamics of opisthorchiasis from 1 month to 1.5 years postinfection in an experimental model based on golden hamsters Mesocricetus auratus.

For the first time, we showed that O. felineus infection provokes time-dependent accumulation of oxidative hepatobiliary lesions in the injured liver of hamsters. In particular, over the course of infection, lipid peroxidation byproducts 4-hydroxynonenal and malondialdehyde were upregulated; these changes in general correlate with the dynamics of hepatic histopathological changes. We detected macrophages with various immunophenotypes and elevated levels of CD68, COX2, and CD163 in the O. felineus–infected animals. Meanwhile, there was direct time-dependent elevation of TNF-α (R = 0.79; p < 0.001) and CD163 protein levels (R = 0.58; p = 0.022). We also provide quantitative data about epithelial hyperplasia marker CK7 and a marker of myofibroblast activation (α smooth muscle actin). Our present data provide first insights into the histopathological mechanism by which O. felineus infection causes liver injuries. These findings support the inclusion of O. felineus in Group 1 of biological carcinogens.
We present an experimental study of the lattice-induced light shifts on the optical clock transition () in neutral ytterbium.

The ``magic'' frequency for the isotope was determined to be , which leads to a first order light shift uncertainty of 0.38 Hz. We also investigated the hyperpolarizability shifts due to the nearby , , and two-photon resonances at 759.708, 754.23, and 764.95 nm, respectively. By measuring the corresponding clock transition shifts near these two-photon resonances, the hyperpolarizability shift was estimated to be 170(33) mHz for a linear polarized, deep, lattice at the magic wavelength.

These results indicate that the differential polarizability and hyperpolarizability frequency shift uncertainties in a Yb lattice clock could be held to well below .
The functions of the hypothalamic adrenal cortical and sympathetic adrenal medullary systems were studied in rats with inherited stress-induced arterial hypertension (ISIAH strain). A characteristic feature of the ISIAH strain is an increase in arterial blood pressure measured both under basal conditions and after restraint stress in particular. In the control ISIAH rats, the basal plasma ACTH concentration was slightly lower than that in the normotensive Wistar albino Glaxo (WAG) rats, and no differences were found in plasma corticosterone. However, the 0.5-h restraint stress produced higher activation of the adrenal cortex in the ISIAH rats. Gluco- and mineralocorticoid responses to the blood volume reduction stresses and ACTH and corticosterone responses to social stress were stronger in the ISIAH than in the control WAG rats. An increase in epinephrine content in adrenals in the basal state and enhanced response of the sympathetic adrenal medullary system to handling stress were observed in the ISIAH rats. Restraint stress produced significantly higher expression of genes encoding corticotropin-releasing hormone-mRNA in hypothalamus and proopiomelanocortin-mRNA in pituitary in the ISIAH than in the WAG rats. Restraint stress produced a decrease in glucocorticoid receptor (GR) gene expression (GR-mRNA) in hippocampus in the ISIAH, but not in the WAG rats. A persistent increase in tyrosine hydroxylase-mRNA in adrenals of the ISIAH rats was found. It is concluded that the ISIAH rat strain is an appropriate model of stress-sensitive hypertension with the predominant involvement of the hypothalamic adrenal cortical and sympathetic adrenal medullary systems in its pathogenesis.
This review article surveys the recent experimental findings that suggest alternative chemical models of directed chirality amplification at the early, prebiotic Earth. It is believed that the chirality amplification step followed the initial emergence of small enantiomeric imbalance and preceded (as a necessary condition) the occurrence of homochiral biopolymers, assembled from enantiomerically pure building blocks. This work focuses on the chemical nature of possible mechanisms of primordial chirality enhancement, without going into detail of the preceding and subsequent phases of origination of biological homochirality and life on Earth. These mechanisms are discussed through the prism of integrity of biological natural selection and chemical kinetic selection.
The work presents results of investigation of couple weak waves with a bow shock at Mach number M = 2.

The waves produced by a small 2D roughness installed on the nozzle inset or side wall of working section. Hot-wire measurements revealed profile of the waves to be similar to N-wave. The visualization was done by means of schlieren technique and interferential AVT SA method.

The inclination angle change of the Mach waves at free-stream section and bow shock section was found.
The root apical meristem (RAM) is the plant stem cell niche which provides for the formation and continuous development of the root. Auxin is the main regulator of RAM functioning, and auxin maxima coincide with the sites of RAM initiation and maintenance. Auxin gradients are formed due to local auxin biosynthesis and polar auxin transport. The PIN family of auxin transporters plays a critical role in polar auxin transport, and two mechanisms of auxin maximum formation in the RAM based on PIN-mediated auxin transport have been proposed to date: the reverse fountain and the reflected flow mechanisms. The two mechanisms are combined here in in silico studies of auxin distribution in intact roots and roots cut into two pieces in the proximal meristem region. In parallel, corresponding experiments were performed in vivo using DR5::GFP Arabidopsis plants. The reverse fountain and the reflected flow mechanism naturally cooperate for RAM patterning and maintenance in intact root. Regeneration of the RAM in decapitated roots is provided by the reflected flow mechanism. In the excised root tips local auxin biosynthesis either alone or in cooperation with the reverse fountain enables RAM maintenance. The efficiency of a dual-mechanism model in guiding biological experiments on RAM regeneration and maintenance is demonstrated. The model also allows estimation of the concentrations of auxin and PINs in root cells during development and under various treatments. The dual-mechanism model proposed here can be a powerful tool for the study of several different aspects of auxin function in root.
The article is devoted to evaluation of the applicability of existing semantic segmentation algorithms for the "Duckietown" simulator. The article explores classical semantic segmentation algorithms as well as ones based on neural networks.

We also examined machine learning frameworks, taking into account all the limitations of the "Duckietown" simulator.

According to the research results, we selected neural network algorithms based on U-Net, SegNet, DeepLab-v3, FC-DenceNet and PSPNet networks to solve the segmentation problem in the "Duckietown" project. U-Net and SegNet have been tested on the "Duckietown" simulator.
Albinism in plants is characterized by lack of chlorophyll and results in photosynthesis impairment, abnormal plant development and premature death. These abnormalities are frequently encountered in interspecific crosses and tissue culture experiments. Analysis of albino mutant phenotypes with full or partial chlorophyll deficiency can shed light on genetic determinants and molecular mechanisms of albinism. Here we report analysis of RNA-seq transcription profiling of barley (Hordeum vulgare L.) near-isogenic lines, one of which is a carrier of mutant allele of the Alm gene for albino lemma and pericarp phenotype (line i:BwAlm). 1221 genome fragments have statistically significant changes in expression levels between lines i:BwAlm and Bowman, with 148 fragments having increased expression levels in line i:BwAlm, and 1073 genome fragments, including 42 plastid operons, having decreased levels of expression in line i:BwAlm. We detected functional dissimilarity between genes with higher and lower levels of expression in i:BwAlm line. Genes with lower level of expression in the i:BwAlm line are mostly associated with photosynthesis and chlorophyll synthesis, while genes with higher expression level are functionally associated with vesicle transport. Differentially expressed genes are shown to be involved in several metabolic pathways; the largest fraction of such genes was observed for the Calvin-Benson-Bassham cycle. Finally, de novo assembly of transcriptome contains several transcripts, not annotated in current H. vulgare genome version. Our results provide the new information about genes which could be involved in formation of albino lemma and pericarp phenotype. They demonstrate the interplay between nuclear and chloroplast genomes in this physiological process.
Animal domestications have led to a shared spectrum of striking behavioral and morphological changes. To recapitulate this process, silver foxes have been selectively bred for tame and aggressive behaviors for over 50 generations at the Institute for Cytology and Genetics in Novosibirsk, Russia.

To understand the genetic basis and molecular mechanisms underlying the phenotypic changes, we profiled gene expression level and coding SNP allele frequencies in two brain tissues from 12 aggressive and 12 tame foxes.

Expression analysis revealed 146 genes in prefrontal cortex and 33 genes in basal forebrain that were differentially expressed (5% FDR). These candidates include genes in key pathways known to be critical to neurological processing, including the serotonin and glutamate receptor pathways. In addition, 295 of the 31,000 exonic SNPs show significant allele frequency differences between tame and aggressive population (1% FDR), including genes with a role in neural crest cell fate determination.
The results of numerical experiments on modeling shock-wave loading of metal composites – elkonites, which are sintered refractory materials (W, WC or Mo) impregnated with fusible metal (Ag or Cu) using thermodynamically equilibrium model TEC (thermodynamic equilibrium components) are presented. Interest in the research of compressibility of such mixtures is associated with the possibility of creating materials with the necessary properties, and with the properties of the materials themselves. The modelling of materials with such components is carried out to develop the promising direction of obtaining materials with specified properties. The good agreement of the model calculations with the data of different authors, which are determined on the basis of experiment, is obtained.