Hengzongshu/ArticleAgent huggingface dataset:
Spin labels selectively attached to biomolecules allow high-accuracy nanoscale distance measurements using pulsed electron paramagnetic resonance (EPR), in many cases providing the only access to the structure of complex biosystems. Triarylmethyl (TAM) radicals have recently emerged as a new class of spin labels expanding the applicability of the method to physiological temperatures.

Using orientation-selective high-frequency (94 GHz) double electron-electron resonance (DEER) we demonstrate strong specific interactions between DNA termini and TAM labels, leading to a significant restriction of their conformational mobility.

An understanding of such interactions guides the way to select optimum TAM-labeling strategies, thus refining nanoscale EPR distance measurements in nucleic acids and their complexes under physiological conditions.
Some substrate properties of analogues of oligothymidylates with p-s-C5′ bonds

Institute of Cytology and Genetics, Siberian Division of the Academy of Sciences

Nucleic Acids Research, Volume 9, Issue 1, 10 January 1981, Pages 189–201
Absolute luminosity measurements are of general interest for colliding-beam experiments at storage rings. These measurements are necessary to determine the absolute cross-sections of reaction processes and are valuable to quantify the performance of the accelerator.

Using data taken in 2010, LHCb has applied two methods to determine the absolute scale of its luminosity measurements for proton-proton collisions at the LHC with a centre-of-mass energy of 7 TeV. In addition to the classic ``van der Meer scan'' method a novel technique has been developed which makes use of direct imaging of the individual beams using beam-gas and beam-beam interactions. This beam imaging method is made possible by the high resolution of the LHCb vertex detector and the close proximity of the detector to the beams, and allows beam parameters such as positions, angles and widths to be determined. The techniques used to transport the absolute luminosity calibration to the full 2010 data-taking period are presented.

The results of the two methods have comparable precision and are in good agreement. Combining the two methods, an overal precision of 3.5% in the absolute luminosity determination is reached.
The KEDR experiment is ongoing at the VEPP-4M e + e − collider at Budker INP in Novosibirsk. The collider center of mass energy range covers a wide spectrum from 2 to 11 GeV. Most of the up-to-date statistics were taken at the lower end of the energy range around the charmonia region. Planned activities at greater energies up to the bottomonia would lead to a significant increase of event recording rates and accelerator backgrounds, thus stressing the existing DAQ and trigger systems beyond their limits.

The described DAQ upgrade plan includes: the redesign of the trigger electronics using modern components to improve the trigger decision time; the development of new readout processors using ethernet connections; new software for collecting events and electronics management; high level of parallelization of data transfers and events processing; improved reliability based on readout computing cluster with redundancy.

The upgraded DAQ system is going to be very flexible and could be considered as a concept prototype of the projected BINP Super Charm-Tau Factory.
An inclusive search for a new-physics signature of lepton-jet resonances has been performed by the ATLAS experiment. Scalar leptoquarks, pair-produced in pp collisions at = 13 TeV at the large hadron collider, have been considered.

An integrated luminosity of 3.2 fb−1, corresponding to the full 2015 dataset was used. First (second) generation leptoquarks were sought in events with two electrons (muons) and two or more jets.

The observed event yield in each channel is consistent with Standard Model background expectations. The observed (expected) lower limits on the leptoquark mass at 95% confidence level are 1100 and 1050 GeV (1160 and 1040 GeV) for first and second generation leptoquarks, respectively, assuming a branching ratio into a charged lepton and a quark of 100%. Upper limits on the aforementioned branching ratio are also given as a function of leptoquark mass. Compared with the results of earlier ATLAS searches, the sensitivity is increased for leptoquark masses above 860 GeV, and the observed exclusion limits confirm and extend the published results.
ВОЗМОЖНОСТИ ОЦЕНКИ СТЕПЕНИ ТЯЖЕСТИ ГЕМОРЕОЛОГИЧЕСКИХ НАРУШЕНИЙ У ЛИЦ С АРТЕРИАЛЬНОЙ ГИПЕРТЕНЗИЕЙ 1
The first observation of the decays ${\overline{B}}_{s}^{0}\ensuremath{\rightarrow}{D}_{s}^{+}{K}^{\ensuremath{-}}{\ensuremath{\pi}}^{+}{\ensuremath{\pi}}^{\ensuremath{-}}$ and ${\overline{B}}^{0}\ensuremath{\rightarrow}{D}_{s}^{+}{K}^{\ensuremath{-}}{\ensuremath{\pi}}^{+}{\ensuremath{\pi}}^{\ensuremath{-}}$ are reported using an integrated luminosity of $1.0\text{ }\text{ }{\mathrm{fb}}^{\ensuremath{-}1}$ recorded by the LHCb experiment. The branching fractions, normalized with respect to ${\overline{B}}_{s}^{0}\ensuremath{\rightarrow}{D}_{s}^{+}{\ensuremath{\pi}}^{\ensuremath{-}}{\ensuremath{\pi}}^{+}{\ensuremath{\pi}}^{\ensuremath{-}}$ and ${\overline{B}}_{s}^{0}\ensuremath{\rightarrow}{D}_{s}^{+}{K}^{\ensuremath{-}}{\ensuremath{\pi}}^{+}{\ensuremath{\pi}}^{\ensuremath{-}}$, respectively, are measured to be $\frac{\mathcal{B}({\overline{B}}_{s}^{0}\ensuremath{\rightarrow}{D}_{s}^{+}{K}^{\ensuremath{-}}{\ensuremath{\pi}}^{+}{\ensuremath{\pi}}^{\ensuremath{-}})}{\mathcal{B}({\overline{B}}_{s}^{0}\ensuremath{\rightarrow}{D}_{s}^{+}{\ensuremath{\pi}}^{\ensuremath{-}}{\ensuremath{\pi}}^{+}{\ensuremath{\pi}}^{\ensuremath{-}})}=(5.2\ifmmode\pm\else\textpm\fi{}0.5\ifmmode\pm\else\textpm\fi{}0.3)\ifmmode\times\else\texttimes\fi{}{10}^{\ensuremath{-}2}$ and $\frac{\mathcal{B}({\overline{B}}^{0}\ensuremath{\rightarrow}{D}_{s}^{+}{K}^{\ensuremath{-}}{\ensuremath{\pi}}^{+}{\ensuremath{\pi}}^{\ensuremath{-}})}{\mathcal{B}({\overline{B}}_{s}^{0}\ensuremath{\rightarrow}{D}_{s}^{+}{K}^{\ensuremath{-}}{\ensuremath{\pi}}^{+}{\ensuremath{\pi}}^{\ensuremath{-}})}=0.54\ifmmode\pm\else\textpm\fi{}0.07\ifmmode\pm\else\textpm\fi{}0.07$, where the first uncertainty is statistical and the second is systematic. The ${\overline{B}}_{s}^{0}\ensuremath{\rightarrow}{D}_{s}^{+}{K}^{\ensuremath{-}}{\ensuremath{\pi}}^{+}{\ensuremath{\pi}}^{\ensuremath{-}}$ decay is of particular interest as it can be used to measure the weak phase $\ensuremath{\gamma}$. First observation of the ${\overline{B}}_{s}^{0}\ensuremath{\rightarrow}{D}_{s1}(2536{)}^{+}{\ensuremath{\pi}}^{\ensuremath{-}}$, ${D}_{s1}^{+}\ensuremath{\rightarrow}{D}_{s}^{+}{\ensuremath{\pi}}^{\ensuremath{-}}{\ensuremath{\pi}}^{+}$ decay is also presented, and its branching fraction relative to ${\overline{B}}_{s}^{0}\ensuremath{\rightarrow}{D}_{s}^{+}{\ensuremath{\pi}}^{\ensuremath{-}}{\ensuremath{\pi}}^{+}{\ensuremath{\pi}}^{\ensuremath{-}}$ is found to be $\frac{\mathcal{B}({\overline{B}}_{s}^{0}\ensuremath{\rightarrow}{D}_{s1}(2536{)}^{+}{\ensuremath{\pi}}^{\ensuremath{-}},{D}_{s1}^{+}\ensuremath{\rightarrow}{D}_{s}^{+}{\ensuremath{\pi}}^{\ensuremath{-}}{\ensuremath{\pi}}^{+})}{\mathcal{B}({\overline{B}}_{s}^{0}\ensuremath{\rightarrow}{D}_{s}^{+}{\ensuremath{\pi}}^{\ensuremath{-}}{\ensuremath{\pi}}^{+}{\ensuremath{\pi}}^{\ensuremath{-}})}=(4.0\ifmmode\pm\else\textpm\fi{}1.0\ifmmode\pm\else\textpm\fi{}0.4)\ifmmode\times\else\texttimes\fi{}{10}^{\ensuremath{-}3}$.
The study investigated essential oils of berries and green biomass of the sea buckthorn at various growth stages.

Plant material of H. rhamnoídes ssp. mongolica was collected in the experimental fields of the Institute of Cytology and Genetics SB RAS (Siberia, Russia). Volatile compounds and essential oils were detected. Green spring shoots without leaves, first-year seedlings without leaves and berries were analyzed. In all studied exemplars, 103 volatile compounds were defined among which considerable proportion wasn't found in a sea-buckthorn earlier.

Phenylpropanoids prevailed in essential oils composition from seedlings. Alkanes and phenylpropanoids prevailed in shoots. Berries contained mostly carboxylic acids and their esters. These data can be applied to a fingerprint of various cultivars of a sea-buckthorn with relevance to food science and technology.
One of the promising approaches of macroeconomic modeling and quantitative assessment of the impact of external and internal factors on macroeconomy of a country, which is actively used abroad, is a Bayesian approach to the description of macroeconomic processes. In this paper we examine Bayesian vector autoregression model (BVAR) to assess the impact of external shocks, such as the price of Brent crude oil, the volatility index VIX and the Shanghai Stock Exchange Composite index, on Russian macroeconomic indicators. The results allow us to estimate the contribution of external factors as a significant in the dynamics of Russia economic variables. This approach can be successfully applied for the analysis of Russian data, which was confirmed by the results presented in the article.
Basic ideas of socio-cognitive approach are considered. This approach explains the moral personality from the viewpoint of availability of cognitive schemas.

The results of testing the experimental model of D. Narvaez, D. Lapsley, S. Hagele and B. Lasky on Russian sample are presented. Primary trait inference method was used to determine if people with availability of moral schemas were more likely to make spontaneous inferences about moral qualities than people with availability of other types of schemas.

The obtained results of the study coincided with the results of D. Narvaez et al. in conclusion that availability of moral constructions affects the processing of social information. People with a greater availability of moral schemas were more likely to make spontaneous inferences about moral qualities than people more oriented on other cognitive schemas.
Evaporation of a sessile nanoliter water droplet was investigated experimentally at the substrate temperature of 30 °C. The studies were performed on the two substrates made of copper.

One of them had no coating and was polished to the root mean square roughness of 20 nm. Another one was coated with single wall nanotubes (the root mean square roughness is 62 nm).

The research has shown that specific rate of evaporation (mass loss per unit of the drop surface area) on the substrate with nanotubes coating is up to 20 percent higher in comparison with the substrate without coating.
The binding of [3H]SCH 23390 has been studied in various brain regions of male mice with the experience of repeated victory (winners) or defeat (losers) gained over 10 (T10) and 20 (T20) days of daily agonistic confrontations.

In the frontal cortex, Bmax of [3H]SCH 23390 binding sites was found to be increased in T10 losers and decreased in T20 losers when compared to the control mice. In the striatum, T10 and T20 winners had reduced values of [3H]SCH 23390 binding sites than the ones in the control mice. The Kd was increased in the frontal cortex of T10 losers and T10 winners as well as in the amygdala of T20 losers. Reduced Kd values were found in the striatum of all experimental groups as well as in the amygdala of T20 winners.

Thus, both specific changes relating to social behavior patterns and non-specific ones in [3H]SCH 23390 binding were found in the brain regions of mice after 10 and 20 days of intermale confrontations.
The combination of atomic spectroscopy, integrated photonics, and microelectromechanical systems leads the way to the demonstration of microcell-based optical atomic clocks. Here, we report the short-term stability budget of table-top Cs microcell-stabilized lasers based on dual-frequency sub-Doppler spectroscopy (DFSDS). The dependence of the sub-Doppler resonance properties on key experimental parameters is studied. The detection noise budget and absolute phase noise measurements are in good agreement with the measured short-term frequency stability of the laser beatnote, at the level of 1.1 × 10 − 12 τ − 1 / 2 until 100 s, currently limited by the intermodulation effect from a distributed-feedback laser setup. The fractional frequency stability of the laser beatnote at 1 s is about 100 times greater than that of commercial microwave chip-scale atomic clocks and validates interest in the DFSDS approach for the development of high-performance microcell-based optical standards.
Background Apurinic/apyrimidinic (AP) endonucleases are key DNA repair enzymes involved in the base excision repair (BER) pathway. In BER, an AP endonuclease cleaves DNA at AP sites and 3′-blocking moieties generated by DNA glycosylases and/or oxidative damage. A Triticum aestivum cDNA encoding for a putative homologue of ExoIII family AP endonucleases which includes E. coli Xth, human APE1 and Arabidopsis thaliana AtApe1L has been isolated and its protein product purified and characterized.

Methodology/Principal Findings We report that the putative wheat AP endonuclease, referred here as TaApe1L, contains AP endonuclease, 3′-repair phosphodiesterase, 3′-phosphatase and 3′→5′ exonuclease activities. Surprisingly, in contrast to bacterial and human AP endonucleases, addition of Mg2+ and Ca2+ (5–10 mM) to the reaction mixture inhibited TaApe1L whereas the presence of Mn2+, Co2+ and Fe2+ cations (0.1–1.0 mM) strongly stimulated all its DNA repair activities. Optimization of the reaction conditions revealed that the wheat enzyme requires low divalent cation concentration (0.1 mM), mildly acidic pH (6–7), low ionic strength (20 mM KCl) and has a temperature optimum at around 20°C. The steady-state kinetic parameters of enzymatic reactions indicate that TaApe1L removes 3′-blocking sugar-phosphate and 3′-phosphate groups with good efficiency (kcat/KM = 630 and 485 μM−1·min−1, respectively) but possesses a very weak AP endonuclease activity as compared to the human homologue, APE1.

Conclusions/Significance Taken together, these data establish the DNA substrate specificity of the wheat AP endonuclease and suggest its possible role in the repair of DNA damage generated by endogenous and environmental factors.
A new mathematical model for the four-point bending of reinforced concrete beams is developed and investigated. The model takes into account multi-modulus concrete behavior, nonlinear stress-strain relationships, and damage evolution.

An algorithm for a numerical implementation of the model is proposed. The corresponding boundary value problem is solved by the hp-version of the least-squares collocation method in combination with an acceleration of an iterative process based on Krylov subspaces and parallelizing. Special attention is given to the influence of mathematical model parameters on the results of numerical simulation.

The results are compared with experimental data and three-dimensional simulation. A satisfactory agreement is shown
For Ni/CeZrO catalyst prepared in supercritical isopropanol main features of methane dry reforming reaction mechanism were studied

by the pulse microcalorimetric technique

The reaction scheme is described by a step-wise redox mechanism with independent stages of CH4 transformation on Ni/support interface producing syngas with participation of support oxygen bridging species (the rate-limiting stage) and fast reoxidation of support sites by CO2 yielding CO regenerating reactive oxygen species
Identified significant changes in the intestinal microbiome in patients with inflammatory bowel disease (IBD), serve as the reason for therapeutic interventions in order to correct it. Faecal microbiota transplantation (TFM) is an effective treatment for recurrent or refractory Clostridium difficile infections, including in patients with inflammatory bowel diseases receiving immunosuppressive and anticytokine therapy.

The results of studying the effectiveness of TFM using a filtered aqueous suspension of donors feces in the correction of intestinal microbiocenosis in patients with recurrent Clostridial infection (RCT), ulcerative colitis (UC) and irritable bowel syndrome (IBS) are presented. 2 weeks after the introduction of the supernatant, a suspension of fecal microorganism microorganisms in patients evaluated the dynamics of the total content of microorganisms and individual representatives of the intestinal microflora.

It was found that the supernatant of an aqueous suspension of donor feces containing microbial exometabolites and other biologically active compounds in the short term has the most pronounced effect on the restoration of normal intestinal microflora only in patients with clostridial infection.
A theoretical approach is proposed for quantitative modeling of SABRE (Signal Amplification by Reversible Exchange) experiments performed using an NMR spectrometer at a high magnetic field. SABRE is a method that exploits the spin order of parahydrogen (the H2 molecule in its nuclear singlet state) for hyper-polarizing the spins of various substrates to enhance their NMR signals. An important feature of SABRE is that the substrate is not modified chemically; instead, spin order transfer takes place in a transient complex with parahydrogen. In high-field SABRE experiments, such a transfer is achieved by using suitable NMR excitation schemes. The approach presented here can explicitly treat the spin dynamics in the SABRE complex as well as the kinetics of substrate exchange (between the free and bound form) and complex interplay of spin evolution and chemical processes. One more important effect included in the model is the alteration of the spin state of parahydrogen giving rise to the formation of anti-phase spin order from the initial singlet order. Such a treatment enables a detailed analysis of known high-field SABRE schemes, quantitative comparison with experiments, and elucidation of the key factors that limit the resulting NMR signal enhancement.
A fully-coupled 3D model of early stage of hydraulic fracture propagation is enriched by incorporating two submodels for simulation of fluid flow inside the fracture.

The Herschel-Bulkley model is used to describe the flow of non-Newtonian fluid and the Reynolds equation is modified for compressible fluid flow simulation. Propagation of inclined hydraulically driven penny shaped fracture in the rock under multiaxial load is simulated.

The influence of the fluid rheology and compressibility on the fluid pressure and the fracture form is shown.
The central geographical position of the Caspian region plays a key role in understanding the historical processes which took place in the territory of Central Asia. One of the important geological factors that influenced the processes of settlement in this region by ancient populations is the transgressive-regressive variability of the Caspian sea level and access to fresh water. There are dozens of Early Holocene sites on the Krasnovodsk Peninsula (Eastern Caspian), most of them being surface complexes. The interpretation of these complexes has so far been controversial due to the lack of stratigraphic context and absolute dating. Purpose. This paper analyzes the techno-typological characteristics of lithic tool assemblages assigned to Early Holocene industries from Eastern Caspian site Sai Djanurpa. The article also discusses the links of how the dynamics of the ancient shores of the Caspian influenced settlement dynamics in the region. Results. The conducted detailed techno-typological analysis of the lithic collection of Sai Djanurpa allowed to discern two distinct complexes. The two complexes defined by different technological and typological characteristics: types of chips, types of tools and metric indicators. The first complex is characterized by the exploitation of chalcedony flint for the production of blades by soft hammer percussion, by borer, end-scraper and retouched flakes in the toolkit. The second complex is associated with the use of chalcedony, sandstone and flint for the production of microbladelets by the pressure technique, from conical and frontal cores. The toolkit of the second set of complexes is characterized by bifacial narrow points, burins, ventral retouched microblades and micro-scrapers. Conclusion. Based on the analysis of the lithic assemblages, the geomorphological position of the site and the paleogeographic condition of the Caspian region, the authors assume that two different populations with two different material cultures inhabited the Sai Djanurpa site during the final Pleistocene – early Holocene and the middle Holocene.
Random field simulation methods that can be used to simulate the stochastic structure of three-dimensional clouds are discussed. Examples of homogeneous random field realizations that reproduce one-dimensional distributions and correlation functions of experimental fields of stratus optical thicknesses are presented.
Rationale: The development of eukaryote genome engineering tools based on CRISPR-Cas programmable bacterial nucleases systems opens wide horizons for gene therapies, human disease cell modeling, as well as investigation into manifestation of disease phenotypes and visualization of cellular processes. The safety and approximation of experiments both at the cellular and organismal levels depend on the accuracy of introducing double-stranded breaks into the target DNA regions. The search for new variants of more accurate CRISPR-Cas nucleases and evaluation of their ability to hydrolyze nucleosome DNA in vivo is considered a critical task for the development of the genome engineering technologies.

Aim: To analyze the activity of the programmable nuclease AsCpf1 (Cas12a), with low level of off-target activity, in the human genome loci that are safe for the introduction of transgenic constructs ('safe harbor') and to compare its efficiency with that of the widely used SpCas9 nuclease in HEK293 cells. Materials and methods: We performed the bioinformatics analysis of the association between target regions with nucleosomes and other proteins in the safe harbor loci AAVS1 and GSH-Ch1 and the transcriptionally inactive gene MYBPC3 (cardiac myosin binding protein 3) based on ATAC-seq data for the HEK293FT cells obtained from the NCBI SRA database. Plasmids encoding SpCas9 and AsCpf1 nucleases and guide RNA to the target regions were constructed and transfected into the HEK293FT cells. Events in the target regions of the HEK293FT cell genome were studied in the sequenograms with the TIDE algorithm.

Results: The results of the ATAC-seq experiments for HEK293FT cells have shown that the AAVS1 locus can be referred as open chromatin with a low nucleosome density, while the GSH-Ch1 locus can be attributed to closed chromatin. In HEK293FT cells, the cardiac MYBPC3 gene has intermediate chromatin density. Assessment of the efficiency of introducing breaks into the studied HEK293FT cell chromatin loci by nucleases has shown that SpCas9 is able to cope with chromatin of any nucleosome density, while AsCpf1 can effectively introduce DNA breaks only at loci with open chromatin, such as AAVS1 and MYBPC3 . Editing events occur at a very low rate at the GSH-Ch1 locus with a high nucleosome density. Conclusion: We have found low efficiency of the AsCpf1 nuclease in the genomic safe harbor locus GSH-Ch1, which is characterized by a high nucleosome density. When planning an experiment on AsCpf1 nuclease genome editing, the epigenetic chromatin landscape and the nucleosome density should be considered, as well as chromatin opening substances should be used.
ПЕРВЫЕ ЭКСПЕРИМЕНТЫ ПО ИНЖЕКЦИИ ЭЛЕКТРОННОГО ПУЧКА В ГАЗОДИНАМИЧЕСКУЮ ЛОВУШКУ