Hengzongshu/ArticleAgent huggingface dataset:
Acipenseriformes take a basal position among Actinopteri and demonstrate a striking ploidy variation among species. The sterlet (Acipenser ruthenus, Linnaeus, 1758; ARUT) is a diploid 120-chromosomal sturgeon distributed in Eurasian rivers from Danube to Enisey. Despite a high commercial value and a rapid population decline in the wild, many genomic characteristics of sterlet (as well as many other sturgeon species) have not been studied.

Cell lines from different tissues of 12 sterlet specimens from Siberian populations were established following an optimized protocol. Conventional cytogenetic studies supplemented with molecular cytogenetic investigations on obtained fibroblast cell lines allowed a detailed description of sterlet karyotype and a precise localization of 18S/28S and 5S ribosomal clusters. Localization of sturgeon specific HindIII repetitive elements revealed an increased concentration in the pericentromeric region of the acrocentric ARUT14, while the total sterlet repetitive DNA fraction (C0t30) produced bright signals on subtelomeric segments of small chromosomal elements. Chromosome and region specific probes ARUT1p, 5, 6, 7, 8 as well as 14 anonymous small sized chromosomes (probes A-N) generated by microdissection were applied in chromosome painting experiments. According to hybridization patterns all painting probes were classified into two major groups: the first group (ARUT5, 6, 8 as well as microchromosome specific probes C, E, F, G, H, and I) painted only a single region each on sterlet metaphases, while probes of the second group (ARUT1p, 7 as well as microchromosome derived probes A, B, D, J, K, M, and N) marked two genomic segments each on different chromosomes. Similar results were obtained on male and female metaphases.

The sterlet genome represents a complex mosaic structure and consists of diploid and tetraploid chromosome segments. This may be regarded as a transition stage from paleotetraploid (functional diploid) to diploid genome condition. Molecular cytogenetic and genomic studies of other 120- and 240-chromosomal sturgeons are needed to reconstruct genome evolution of this vertebrate group.
The characteristic time of disappearance of electric field in massive electrodynamics during the capture of electric charge by black holes is calculated.

It is shown that this time does not depend upon the photon mass.

The electric field at large distances disappears with the speed of light.
Though hundreds of thousands of papers are currently being published on HIV/AIDS, only tens of hundreds of them are devoted to the antibodies generated during the disease. Most of these papers discuss antibodies in HIV/AIDS as a diagnostic tool, and some articles describe neutralizing antibodies as a promising treatment. In this paper, we used affinity chromatography and ELISA to isolate natural IgG from the blood of 26 HIV-infected patients. IgG preparations were separated into the subfractions containing different types of light chains, and catalytic activities of subfractions were analyzed. Here, we show for the first time that the blood of HIV patients contains ~20% of bispecific κλ-IgG, presented with all IgG subclasses. Analysis of DNA-hydrolyzing and amylolytic activity show that most IgG preparations and subfractions are catalytically active. Our results expand the possible biological functions of natural IgG in HIV infection.
Strong explosive eruptions of volcanoes throw out mixtures of gases and ash from high-pressure underground reservoirs. Investigating these subsurface reservoirs may help to forecast and characterize an eruption.

In this study, we compare seismic tomography results with remote sensing and petrology data to identify deep and subaerial manifestations of pre-eruptive processes at Bezymianny volcano in Kamchatka shortly before its violent explosion on December 20, 2017. Based on camera networks we identify precursory rockfalls, and based on satellite radar data we find pre-eruptive summit inflation. Our seismic network recorded the P and S wave data from over 500 local earthquakes used to invert for a 3D seismic velocity distribution beneath Bezymianny illuminating its eruptive state days before the eruption.

The derived tomography model, in conjunction with the presence of the high-temperature-stable SiO2 polymorph Tridymite in juvenile rock samples, allowed us to infer the coexistence of magma and gas reservoirs revealed as anomalies of low (1.5) and high (2.0) Vp/Vs ratios, respectively, located at depths of 2–3 km and only 2 km apart. The reservoirs both control the current eruptive activity: while the magma reservoir is responsible for episodic dome growth and lava flow emplacements, the spatially separated gas reservoir may control short but powerful explosive eruptions of Bezymianny.
Установлена формула площади для отображений-графиков на двуступенчатых сублоренцевых структурах с произвольным количеством пространственных и временных направлений. Для отдельного случая структур рассмотрен альтернативный подход, не требующий дополнительной гладкости отображения, по которому строится график. Библиография: 40 наименований.
Cervical cancer screening is based on cytologic analysis and high-risk human papillomavirus (HR-HPV) testing, each having their drawbacks. Implementation of new biomarker-based methods may improve screening accuracy. Here, the levels of 25 microRNAs (miRNAs, miRs) and 12 mRNAs involved in cervical carcinogenesis in 327 air-dried Papanicolaou-stained cervical smears from patients with cervical precancerous lesions, cancer, or without the disease were estimated by real-time PCR. Using logistic regression analysis, small-scale miRNA-based, mRNA-based, and combined molecular classifiers were built based on paired ratios of miRNA or mRNA concentrations; their ability to detect high-grade cervical lesions and cancer was then compared. Combined mRNA-miRNA classifiers manifested a better combination of sensitivity and specificity than miRNA- and mRNA-based classifiers. The best classifier, combining miR-375, miR-20, miR-96, CDKN2A, TSP4, and ECM1, predicted high-grade lesions with diagnostic sensitivity of 89.0%, specificity of 84.2%, and a receiver-operating characteristic area under the curve of 0.913. Additionally, in a subsample of the same specimens, the levels of MIR124-2 and MAL promoter methylation, HR-HPV genotypes, and viral loads were analyzed. The relative high-grade lesion risk estimated by the classifier correlated with the frequency of MAL and MIR124-2 methylation but not with the HR-HPV genotype or viral load. The results support the feasibility of cellular biomarker-based methods for cervical screening and patient management.
Here ultra-parabolic equations are linked with diffusion processes with inertia (convective heat transfer) on a shock wave front.

In the present paper, we derive the kinetic equation and impulsive condition and formulate a class of kinetic solutions to impulsive ultra-parabolic equations.
Generation of induced pluripotent stem (iPS) cells expanded possibilities of pluripotency and early development studies. Generation of order Carnivora iPS cells from dog ( Canis lupus familiaris ), snow leopard ( Panthera uncia ), and American mink ( Neovison vison ) was previously reported. The aim of the current study was to examine conditions of pinniped fbroblast reprogramming. Pinnipeds are representatives of the suborder Caniformia sharing conservative genomes. There are several ways to deliver reprogramming transcription factors: RNA, proteins, plasmids, viral vectors etc. The most eﬀective delivery systems for mouse and human cells are based on viral vectors. We compared a lentiviral vector which integrates into the genome and a Sendai virus­based vector, CytoTune EmGFP Sendai Fluorescence Reporter. The main advantage of Sendai virus­based vectors is that they do not integrate into the genome. We performed delivery of genetic constructions carrying ﬂuorescent proteins to fbroblasts of seven Pinnipeds: northern fur seal ( Callorhinus ursinus ), Steller sea lion ( Eumetopias jubatus ), walrus ( Odobenus rosmarus ), bearded seal ( Erignathus barbatus ), Baikal seal ( Pusa sibirica ), ringed seal ( Phoca hispida ), and spotted seal ( Phoca largha ). We also transduced American mink ( N. vison ), human ( Homo sapiens ), and mouse ( Mus musculus ) fbroblasts as a control. We showed that the Sendai virus­based transduction system provides transgene expression one­two orders of magnitude higher than the lentiviral system at a comparable multiplicity of infection. Also, transgene expression after Sendai virus­based transduction is quite stable and changes only slightly at day four compared to day two. These data allow us to suggest that Sendai virus­based vectors are preferable for generation of Pinniped iPS cells.
Ribosomal proteins are involved in many cellular processes through interactions with various RNAs.

Here, applying the photoactivatable-ribonucleoside-enhanced cross-linking and immunoprecipitation approach to HEK293 cells overproducing ribosomal protein (rp) eS1, we determined the products of RNU5A-1 and RNU11 genes encoding U5 and U11 snRNAs as the RNA partners of ribosome-unbound rp eS1. U11 pre-snRNA-associated rp eS1 was revealed in the cytoplasm and nucleus where rp eS1-bound U11/U12 di-snRNP was also found. Utilizing recombinant rp eS1 and 4-thiouridine-containing U11 snRNA transcript, we identified an N-terminal peptide contacting the U-rich sequence in the Sm site-containing RNA region. We also showed that the rp eS1 binding site on U11 snRNA is located in the cleft between stem-loops I and III and that its structure mimics the respective site on the 18S rRNA.

It was found that cell depletion of rp eS1 leads to a decrease in the splicing efficiency of minor introns and to an increase in the level of U11 pre-snRNA with the unprocessed 3' terminus. Our findings demonstrate the engagement of human rp eS1 in events related to the U11 snRNA processing and to minor-class splicing. Contacts of rp eS1 with U5 snRNA in the minor pre-catalytic spliceosome are discussed.
Physical effects scale differently with size. As a necessary preliminary step toward geophysically significant extrapolations, an experimental and numerical study of the scale effects in internal wave attractors in linear and nonlinear regimes is made, showing two different scalings for the beam width.
This Special Issue of Minerals covers a broad range of topics related to the mineralogy of noble metals (Au, Ag, Pt, Pd, Rh, Ru) and the forms of occurrence, formation and distribution of these elements in natural ore-forming systems
We report magnetotransport measurements in a HgTe quantum well with an inverted band structure, which is expected to be a two-dimensional (2D) topological insulator. A small magnetic field perpendicular the 2D layer breaks the time-reversal symmetry and thereby suppresses the edge state transport. A linear magnetoresistance is observed in low magnetic fields when the chemical potential moves through the bulk gap. That magnetoresistance is well described by numerical calculations of the edge state magnetotransport in the presence of nonmagnetic disorder. With the magnetic field increasing, both the local and nonlocal resistances first sharply decrease and then increase again in disagreement with the existing theories.
The antioxidant system (AOS) maintains the optimal concentration of reactive oxygen species (ROS) in a cell and protects it against oxidative stress. In plants, the AOS consists of seven main classes of antioxidant enzymes, low-molecular antioxidants (e.g., ascorbate, glutathione, and their oxidized forms) and thioredoxin/glutaredoxin systems which can serve as reducing agents for antioxidant enzymes. The number of genes encoding AOS enzymes varies between classes, and same class enzymes encoded by different gene copies may have different subcellular localizations, functional loads and modes of evolution. These facts hereafter reinforce the complex nature of AOS regulation and functioning. Further studies can describe new trends in the behavior and functioning of systems components, and provide new fundamental knowledge about systems regulation. The system is revealed to have a lot of interactions and interplay pathways between its components at the subcellular level (antioxidants, enzymes, ROS level, and hormonal and transcriptional regulation). These facts should be taken into account in further studies during the AOS modeling by describing the main pathways of generating and utilizing ROS, as well as the associated signaling processes and regulation of the system on cellular and organelle levels, which is a complicated and ambitious task. Another objective for studying the phenomenon of the AOS is related to the influence of cell dynamics and circadian rhythms on it. Therefore, the AOS requires an integrated and multi-level approach to study. We focused this review on the existing scientific background and experimental data used for the systems biology research of the plant AOS.
Chiasma distributions along bivalents 1 and 14 in female and male mice were studied. It was shown that the average chiasma number in both chromosomes show no sex difference.

By means of a computer simulation it was demonstrated that the differences in the average recombination frequency result from differences in chiasma distribution.

There are however, significant sex differences in chiasma distribution along 1 and 14 chromosomes. In males there are two terminal chiasma peaks in chromosome 1 and one subtelomeric peak of chiasmata in chromosome 14. In females chiasma distributions are more even. According to genetic data, females produce more recombinants between loci of chromosome 1 than males do.
Purpose. Development of electronic educational resources in the humanitarian environment of higher school is an urgent problem of modern education. The aim of our work is to systematize didactic forms and approaches to the development of e-learning resources as exemplified by the interactive educational and methodological complex “Prehistoric Art of Siberia and the Far East”. Results. We describe models of electronic tutorials in terms of a modern direction, development of interactive teaching complexes. An original proprietary information system “Prehistoric Art of Siberia and the Far East” (mobileart.artemiris.org) contains information on the monuments of the Paleolithic age of Siberia, which is presented in a systematic way. Objects of the Siberian Paleolithic collections are attributed, provided with a scientific description, photographs, macro, three-dimensional models and a list of references. Conclusion. Modern education at the university supposes a wide usage of information technology. Interactive educational and methodological complexes today can fully satisfy this need. 3D technology proposed for presenting archaeological materials not only provides effective illustrations of the context, but also implements a new, more sophisticated methodology for studying historical and cultural heritage. Enhanced capabilities of working with a virtual model of archaeological objects allow researchers and students to get more information remotely, which reduces the anthropogenic load on the object under study. The complex developed implies non-contact processing of museum exhibits with varying degrees of damage, using computer simulation techniques for the pre-excavation state of reconstruction, provides an opportunity to restore badly damaged or even gone objects and to preserve the model shape with a possibility of subsequent replication. All of the above forces us to open a new course in the educational programs for archaeology students. It is assumed that this educational segment will find its place in the new Federal State Educational Standard and will contribute to the formation of the competencies of a modern specialist.
The recent precise measurements of the e + e − → K S K L and e + e − → K + K − cross sections and the hadronic spectral function of the τ − → K − K S ν τ decay

are used to extract the isoscalar and isovector electromagnetic kaon form factors and their relative phase in a model independent way

The experimental results are compared with a fit based on the vector-meson-dominance model
The aim of this work was to identify genetic variants potentially involved in familial hypercholesterolemia in 43 genes associated with lipid metabolism disorders.

Targeted high-throughput sequencing of lipid metabolism genes was performed (80 subjects with a familial-hypercholesterolemia phenotype). For patients without functionally significant substitutions in the above genes, multiplex ligation-dependent probe amplification was conducted to determine bigger mutations (deletions and/or duplications) in the LDLR promoter and exons.

A clinically significant variant in some gene associated with familial hypercholesterolemia was identified in 47.5% of the subjects. Clinically significant variants in the LDLR gene were identified in 19 probands (73.1% of all variants identified in probands); in three probands (11.5%), pathogenic variants were found in the APOB gene; and in four probands (15.4%), rare, clinically significant variants were identified in genes LPL, SREBF1, APOC3, and ABCG5. In 12 (85.7%) of 14 children of the probands, clinically significant variants were detectable in genes associated with familial hypercholesterolemia. The use of clinical criteria, targeted sequencing, and multiplex ligation-dependent probe amplification makes it possible to identify carriers of rare clinically significant variants in a wide range of lipid metabolism genes and to investigate their influence on phenotypic manifestations of familial hypercholesterolemia.
The aim of this work is to study the possibility of using blood serum (BS) fatty acids (FA) and erythrocytes (ER) as diagnostic markers of the severity of NAFLD.

Materials and methods. We examined 52 patients with NAFLD (51.8 ± 3.9 years), confirmed by the NLFS index, and 20 apparently healthy men (49.2 ± 4.5 years). The degree of liver fibrosis was established by indirect elastometry (FibroScan® 502 Echosens, France). 27 patients had an initial degree of fibrosis (F0-1), 25 had severe fibrosis (F2-4). The study of the composition of fatty acids of Er and BS was carried out using a GC / MS system based on three Agilent 7000B quadrupoles (USA). Results. Significant differences in the levels of fatty acids in blood serum and erythrocyte membranes in patients with NAFLD were revealed, associated with the degree of fibrosis and necroinflammatory activity. To distinguish between mild and severe fibrosis in NAFLD, the levels of saturated fatty acids (myristic, pentadecane, margarine) and omega-3 PUFAs (eicosapentaenoic, docosapentaenoic, docosahexaenoic) were found to be significant (p = 0.002-0.0003). Saturated and monounsaturated FAs (palmitelaidic, palmitoleic, vaccenic) played a key role in differentiating the degree of necroinflammatory activity (minimal versus pronounced) (p = 0.03-0.005). The created diagnostic panels (FA of blood serum and erythrocyte membranes) made it possible to differentiate patients with NAFLD with varying degrees of fibrosis. Correlations of FA levels in erythrocyte membranes and blood serum with manifestations of metabolic syndrome, indicators of liver damage in patients with NAFLD were revealed.

Conclusions. The established differences in fatty acid profiles of blood serum and erythrocyte membranes in patients with NAFLD, associated with the degree of fibrosis, necroinflammatory activity, manifestations of metabolic syndrome and indicators of liver damage, should be considered as promising biomarkers for assessing the severity of NAFLD.
The article describes one of the important directions of increasing the level of innovative development of the oil and gas complex of Russia - the development of hard-to-recover hydrocarbon reserves (HTR). The search, exploration and development of hard-to-recover reserves can bring the domestic oil and gas and related industries to a new level of technological development, provide a significant multiplier effect for related industries, and create new jobs and infrastructure. The authors estimated the share of HTR in modern oil production, analyzed the dynamics and structure of hydrocarbons of special quality and conditions.
The paper deals with the development of multi-agent systems to support the implementation of projects of transcontinental highways. Decisions on transport routes are more influenced by the weight, trust of experts, geostrategists, various government agencies, think-centers, etc., as well as the information field that is formed as this topic is covered in the media, including on the Internet. space. The statement of the problem and the formalization of the multi-agent system was carried out on the example of transport projects in Central and Central Asia. The article provides a formalized model and characteristics of the role of individual elements of the environment and the agents themselves.
Recently CMS and ATLAS announced that they had measured the Higgs boson parity. In this note we show that their approach can determine this parity only under the additional assumption that an extension of Standard Model of some special type is realized in Nature.
In the paper, we consider a problem of clustering a finite set of N points in d-dimensional Euclidean space into two clusters minimizing the sum over all clusters of the intracluster sums of the distances between clusters elements and their centers. The center of one cluster is defined as centroid (geometric center). The center of the other one is a sought point in the input set. We analyze the variant of the problem with the given clusters sizes. We have proved the strong NP-hardness of this problem.
Implicitly, the same assumptions were employed to obtain the charmonium leptonic width and the absolute branching fractions in many experiments.

A high-precision determination of the main parameters of the \psi(2S) resonance has been performed with the KEDR detector at the VEPP-4M e^{+}e^{-} collider in three scans of the \psi(2S) -- \psi(3770) energy range. Fitting the energy dependence of the multihadron cross section in the vicinity of the \psi(2S) we obtained the mass value M = 3686.114 +- 0.007 +- 0.011 ^{+0.002}_{-0.012} MeV and the product of the electron partial width by the branching fraction into hadrons \Gamma_{ee}*B_{h} = 2.233 +- 0.015 +- 0.037 +- 0.020 keV. The third error quoted is an estimate of the model dependence of the result due to assumptions on the interference effects in the cross section of the single-photon e^{+}e^{-} annihilation to hadrons explicitly considered in this work.

Using the result presented and the world average values of the electron and hadron branching fractions, one obtains the electron partial width and the total width of the \psi(2S): \Gamma_{ee} =2.282 +- 0.015 +- 0.038 +- 0.021 keV, \Gamma = 296 +- 2 +- 8 +- 3 keV. These results are consistent with and more than two times more precise than any of the previous experiments.