Hengzongshu/ArticleAgent huggingface dataset:
Human apurinic/apyrimidinic endonuclease 1 (APE1) has multiple functions in base excision DNA repair (BER) and other cellular processes. Its eukaryote-specific N-terminal extension plays diverse regulatory roles in interaction with different partners. Here, we explored its involvement in interaction with canonical BER proteins. Using fluorescence based-techniques, we compared binding affinities of the full-length and N-terminally truncated forms of APE1 (APE1NΔ35 and APE1NΔ61) for functionally and structurally different DNA polymerase β (Polβ), X-ray repair cross-complementing protein 1 (XRCC1), and poly(adenosine diphosphate (ADP)-ribose) polymerase 1 (PARP1), in the absence and presence of model DNA intermediates. Influence of the N-terminal truncation on binding the AP site-containing DNA was additionally explored. These data suggest that the interaction domain for proteins is basically formed by the conserved catalytic core of APE1. The N-terminal extension being capable of dynamically interacting with the protein and DNA partners is mostly responsible for DNA-dependent modulation of protein–protein interactions. Polβ, XRCC1, and PARP1 were shown to more efficiently regulate the endonuclease activity of the full-length protein than that of APE1NΔ61, further suggesting contribution of the N-terminal extension to BER coordination. Our results advance the understanding of functional roles of eukaryote-specific protein extensions in highly coordinated BER processes.
The trivial lower bound for the 2-distance chromatic number χ 2 (G) of any graph G with maximum degree ∆ is ∆ + 1.It is known that χ 2 = ∆ + 1 if the girth g of G is at least 7 and ∆ is large enough.There are graphs with arbitrarily large ∆ and g ≤ 6 having χ 2 (G) ≥ ∆ + 2. We prove the 2-distance 4-colorability of planar subcubic graphs with g ≥ 22.
Long term ground stability is essential for achieving the performance goals of the Next Linear Collider.

To characterize ground motion on relevant time scales, measurements have been performed at three geologically different locations using a hydrostatic level system developed specifically for these studies.

Comparative results from the different sites are presented in this paper.
Isolated glycogen storage disease of the heart (PRKAG2 syndrome) is a form of glycogenosis, which is characterized by left ventricular hypertrophy, similar to the phenotype of hypertrophic cardiomyopathy, associated with pre-excitation of the ventricles and conduction disorders. The disease is caused by mutations in the gene PRKAG2 encoding for the 5’Adenosine Monophosphate-Activated Protein Kinase (AMPK), specifically for its y2 regulatory subunit, inheritance — autosomal dominant.

A review of the literature data and clinical observation of two patients from the same family with the mutation c905C>A (p.Arg302Gln) in the PRKAG2 gene associated with WPW syndrome and early development of conduction disorders requiring implantation of a pacemaker are presented.

The issues of diagnosis and treatment strategy of the disease were discussed.
Duchenne muscular dystrophy (DMD) is a severe and rapidly progressive hereditary muscular disease with X-linked recessive inheritance, occurring mainly in males. A complete loss of dystrophin resulted from out-of-frame deletion mutations in the DMD gene leads to Duchenne muscular dystrophy. DMD induced pluripotent stem cells (iPSCs) are a suitable cell model to study muscle development and disease mechanisms underlying muscular dystrophy and to screen novel compounds with potential therapeutic effects. We generated iPSCs from a DMD patient using non-integrating episomal plasmid vectors. The obtained iPSC lines showed ESC-like morphology, expression pluripotency markers, displayed a normal karyotype and possessed trilineage differentiation potential.
The thermosalient effect (crystal jumping on heating) attracts much attention as both an intriguing academic phenomenon and in relation to its potential for the development of molecular actuators but its mechanism remains unclear. 1,2,4,5-Tetrabromobenzene (TBB) is one of the most extensively studied thermosalient compounds that has been shown previously to undergo a phase transition on heating, accompanied by crystal jumping and cracking. The difference in the crystal structures and intermolecular interaction energies of the low- and high-temperature phases is, however, too small to account for the large stress that arises over the course of the transformation.

In the present work, the anisotropy of lattice strain is followed across the phase transition by single-crystal X-ray diffraction, focusing on the structural evolution from 273 to 343 K. A pronounced lattice softening is observed close to the transition point, with the structure becoming more rigid immediately after the phase transition. The diffraction studies are further supported by theoretical analysis of pairwise intermolecular energies and zone-centre lattice vibrations.

Only three modes are found to monotonically soften up to the phase transition, with complex behaviour exhibited by the remaining lattice modes. The thermosalient effect is delayed with respect to the structural transformation itself. This can originate from the martensitic mechanism of the transformation, and the accumulation of stress associated with vibrational switching across the phase transition. The finding of this study sheds more light on the nature of the thermosalient effect in 1,2,4,5-tetrabromobenzene and can be applicable also to other thermosalient compounds.
A search is performed for anomalous interactions of the recently discovered Higgs boson using matrix element techniques with the information from its decay to four leptons and from associated Higgs boson production with two quark jets in either vector boson fusion or associated production with a vector boson.

The data were recorded by the CMS experiment at the LHC at a center-of-mass energy of 13TeV and correspond to an integrated luminosity of 38.6fb−1. They are combined with the data collected at center-of-mass energies of 7 and 8TeV, corresponding to integrated luminosities of 5.1 and 19.7fb−1, respectively.

All observations are consistent with the expectations for the standard model Higgs boson.
Intensity of hydrogen Balmer-alpha line is sensitive to distribution of populations of sublevels of excited state due to difference of radiative decay branching ratio for sublevels with different orbital quantum number. Since these sublevels are degenerate transitions between sublevels and mixing of its population may be caused by very weak action of environment.

A beam of fast hydrogen atoms is a system in which these fine effects of population mixing can be observed. Experimental data of Hα line radiation intensities in hydrogen neutral beams are compared with calculations assumed different models of sublevel population.

The comparison most probably point out that population mixing effects cause transitions from long-lived 3s to 3p sublevel with corresponding changes in Hα radiation intensities. Discussed effects may influence to results of Doppler-shift spectroscopy measurements of the neutral beam parameters such as species and impurities content and neutralizing efficiency.
Spanning across thousands of years of aromatic culture development in the countries of East Asia, incense application has accumulated vast experience in various fields, including calendrical calculations and time measurement. Analysis of artifacts, objects of religious worship and everyday life indicates fragrant substances’ great versatility in terms of their use: in addition to sticks, spirals and cones, devices such as the Hundred Graduations Incense seals, alarm clocks, as well as clocks that measured night time, strictly dependent on the calendar season, were invented.

The study of the ways of using incense in East Asian countries (including for measuring time) is based on the analysis of a variety of sources ‒ written, artistic and ethnographic. Compared to the large number of Chinese and Japanese sources, the cultural heritage of the Korean Peninsula contains significant gaps, which significantly complicates the interpretation of the material.

The use of incense burners, aromatic raw materials and various instruments for measuring time is a remarkable phenomenon in the fragrance culture of China, Korea and Japan, testifying to the high adaptability of symbols and images of traditional culture not only to everyday household needs, but also to various achievements of science and technology. This is confirmed at the present time, given the production of new models of aromatic clocks.
Heat pipes are a promising cooling system due to the fact of high efficiency and non-availability of moving parts such as pumps. The experimental investigation of heat transfer in a flat heat pipe has been carried out. The temperature field on the heat pipe has been evaluated using an infrared camera and K-type thermocouples. The IR-camera data and K-type thermocouple data have been compared. The linear dependence of the power supplied to the evaporator on the temperature difference between the evaporator and the condenser is shown.
Exfoliation of metal-organic frameworks is one of the technological strategy for fabrication of complex metal- organic structures for catalysis and energy storage. Here we report on exfoliation of 2D metal-organic frameworks in ultrasonic bath for creating monolayers for further investigation of morphology and mechanics of the layers by atomic force microscopy.
Новосибирский государственный университетНовосибирск, Россия Аннотация
A method proposed to preserve the electron beam polarization at the VEPP-4M collider during acceleration with crossing the integer (imperfection) spin resonance at energy $E=1763\text{ }\text{ }\mathrm{MeV}$ has been successfully applied. It is based on full decompensation of the $0.6\ifmmode\times\else\texttimes\fi{}3.3\text{ }\text{ }\mathrm{Tesla}\ifmmode\times\else\texttimes\fi{}\text{meter}$ integral of the KEDR detector longitudinal magnetic field due to the antisolenoid being ``switched off.''
The fi rst joint study by Russian and Philippine archaeologists addresses an unusual variant of a burial tradition distributed in Island Southeast Asia – burials in anthropomorphic clay jars, found in Ayub Cave (southern Mindanao Island, Philippines), excavated by specialists from the National Museum of the Philippines in 1991–1992, and tentatively dated to 500 BC to 500 AD.

Of special interest are lids of jars shaped as painted human heads with individualized facial features and expressions. The fi nds suggest that Ayub Cave was a necropolis of the tribe elite, and that vessels were produced by a special group of potters using elaborate “prestige technologies”.

The Ayub ceramic collection has various parallels relating to clay fi gurines and decoration including painting, among Late Neolithic and Early Metal Age assemblages from the Philippines (Luzon, Palawan, and Negros Islands), Indonesia (Sumba, Flores, and Bali Islands), and other regions of the Pacifi c Basin from Japan (Jomon) and Korea (Early Iron Age burials) to Vanuatu Islands (Lapita culture). These parallels suggest that the source of the anthropomorphic symbolism was the Austronesian migration with one of its routes passing from southern China via Taiwan, the northern Philippines, Mariana Islands, and further south to Melanesia and Polynesia.
The article is devoted to the study of the theory of naive linguistics and the principles of its research.

The material of the research is the results of psycholinguistic experiment. The words denoting flora and fauna (bionyms) were used as the stimuli for the experiment. The responses in the experiment have revealed a specific character of word functioning in the language consciousness of Mongolian native speakers. To describe a naive view on a word meaning, the respondents used associations in the form of spontaneous definitions and citations that include the word stimulus as well as comparisons it inspired.

The research has made it possible to reconstruct a fragment of the ethnical language world view as a component of the language consciousness of Mongolian native speakers. In the conclusion the author gives a description of these bionyms, compiled on the basis of lexicographic processing of reactions and within the framework of the naive linguistics theory.
Reprogramming of somatic cells to a pluripotent state is a complex, multistage process that is regulated by many factors. Among these factors, non-coding RNAs and microRNAs (miRNAs) have been intensively studied in recent years. MiRNAs play an important role in many processes, particularly in cell reprogramming.

In this study, we investigated the reprogramming of rat fibroblasts with a deleted locus encoding a cluster comprising 14 miRNAs (from miR-743a to miR-465). The deletion of this locus was demonstrated to decrease significantly the efficiency of the cell reprogramming. In addition, the cells produced by the reprogramming differed from rat embryonic and induced pluripotent stem cells, which was an indication that reprogramming in these cells had not been completed.

We suggest that this miRNA cluster or some of its members are involved in regulating the reprogramming of rat cells to a pluripotent state.
The present experimental work reports on the temperature measurements in a Bunsen flame, covered by a flat cooled plate from the top.

The temperature field is measured based on the planar laser-induced fluorescence method for hydroxyl radical (OH) during the excitation of excitation Q 1 (8) line of the (1-0) band of the A 2 Σ + -X 2 Π electronic system. Thermometry was based on the imaging of the ratio of the fluorescence intensity for the band (2-0) and bands (0-0), (1-1). The paper presents the obtained temperature fields for the distances between the plate and the burner of 1, 2 and 3 calibres.

The paper also concludes that the used method can be implemented using a single-pulse laser illumination and is effective for the detection of cold zones.
A novel ultra-high-energy cosmic rays energy and arrival direction reconstruction method for Telescope Array surface detector is presented.

The analysis is based on a deep convolutional neural network using detector signal time series as the input and the network is trained on a large Monte-Carlo dataset.

This method is compared in terms of statistical and systematic energy and arrival direction determination errors with the standard Telescope Array surface detector event reconstruction procedure.
Smart city technologies are designed to make human life easier and more comfortable. However, in reality, there haven’t yet been developed unambiguous criteria for measuring the effectiveness of smart city initiatives. This means that the city residents’ needs fade into the background.

This article uses such techniques as expert interviews (with municipal representative, IT businessman, sociologist, economist and urban researcher) as well as semi-formalized interviews with residents of Novosibirsk. The study describes key measurements of the smart city effectiveness (Physical environment, Society, Government, Technology & Data) and examines in detail two of them: Society and Government.

The research also indicates the main problems and obstacles to the effective implementation of the smart city program for each of these dimensions. The study has scientific importance, as it describes the contradictions between government and society when trying to introduce smart technologies. Also the results of the study may help IT companies to pay attention on people’s needs when developing and promoting smart applications and services.
Due to their high solubility and stability, neonicotinoid insecticides are able to accumulate in water bodies, affecting aquatic organisms. The aims of this study were to evaluate resistance (LC50) of Anopheles messeae s.l. (Anopheles messeae and An. daciae) to the neonicotinoid imidacloprid and to search for genetic markers associated with insecticide resistance. The LC50 values of these species in the collections during 2017 and 2018 were indistinguishable and were in the range of 0.027–0.051 mg/l. In general, the LC50 values of the mosquitoes were comparable with values of other mosquito species of the Anopheles and Culex genera. Gene polymorphisms of the variations in intron lengths and the presence of restriction sites in introns that were potentially associated with the metabolism of insecticides were studied. Polymorphisms of the studied genes in the pair of closely related species considered overlapped, but allele frequencies were different. Part of the genetic variants arose due to insertions of repetitive elements of the genome. Two variants of the cytochrome P450 gene Cyp6AG1 in An. daciae were associated with increased resistance to imidacloprid. Possible side effects of selection on insecticide resistance in blood-sucking mosquitoes are discussed.
This article is devoted to the analysis of the Advanced development zone's (ADZ) initiative in the context of the economic integration of the Russian Federation and South Korea. The author of the article, relying on an extensive source base, for the first time attempts to answer the question of what factors impede the successful implementation of projects by the two countries within the framework of the ADZ initiative.

The study was carried out within the framework of an integrated approach using the SCAN Interfax media environment analysis system as one of the main forecasting tools. At the same time, the author draws on statistical data reflecting the state of export-import, trade turnover between the two countries over the past five years.

The analysis of the source made it possible to determine that despite the huge interest of South Korean business in the emerging markets of the Russian Federation, due to the complicated administrative procedures, the fundamental difference in the procedure for investing in high-tech and commercial production, lack of trust in the Russian system of economic planning, insufficient study of ways of doing business with South Korean companies with On the part of Russian Far Eastern specialists, the project could not be implemented as planned.
In this Letter we report an observation of interference effects in Compton scattering in the experiment held on the VEPP-2000 collider. Infrared laser radiation was scattered head-on the 990 MeV electrons inside the dipole magnet, where an electron orbit radius is about 140 cm. It was observed that the energy spectrum of backscattered photons, measured by a HPGe detector, differs from that defined by the Klein-Nishina cross section and scattering kinematics of free electrons. The explanation of the effect, proposed in terms of classical electrodynamics, is in agreement with QED calculations.
In the last 20 years, multilayer optics for x-ray and EUV applications have revolutionized the measurement capabilities and the experimental set ups of synchrotron beamlines. Rigaku Innovative Technologies (formerly Osmic) has pioneered these technologies by designing, engineering and manufacturing of distinctive multilayer coatings. We are experienced and internationally recognized for our innovative technologies.We control our production of specified internal roughness on the atomic level while creating layered materials of defined thickness. These multilayers have d-spacings, synonymous for layer-pair thickness, which we can produce uniform, laterally graded, depth graded or even a combination of both gradients. We guarantee our specifications and we have a wide range of metrology technology in house, enabling us to keep our promised specifications and to expand the technical boundaries in our field of applications. Our core capabilities are the detailed knowledge of distinctive multilayer coating technology including the mounting and alignment of optics. We do direct coating on prefigured substrates or, mostly for applications in analytical laboratories, bonding of thin substrates to flat or curved backings. Having delivered thousands of multilayer structures to the analytical scientific market, we are able to design and engineer multilayers on the basis of numerical and analytical simulations of thickness, roughness, required densities and other optical parameters. This technology gives us the possibility to produce just as one example multilayers with a period of 15 Angstroms, a reflectivity of much better than 30% and an energy resolution of better than 0.4%. We also have coatings and multilayer structures for substrates up to 1400 mm length and 400 mm width. The number of chemically different coatings we are able to perform is very broad. This is starting with the classical W/Si through Mo/B4C to a lot of other material combinations. We also design and produce dual wavelength multilayers, narrowand broad-bandwidth multilayers for energy ranges from 40ev to 40 keV. Please see figure (1) for further details. Here we have shown our calculated values for two different multilayer sandwiches. Further material will be shown. Our actual production quality will also be presented.
The cross section of the process e + e − → K S K L π 0 has been studied with the CMD-3 detector at the VEPP-2000 electron-positron collider in the center-of-mass energy range from 1.1 to 2 GeV.

Preliminary results on the total cross section of the process are presented.
Berberine is known for its wide range of biological activity, including hypolipidemic, antibacterial, hypoglycemic, etc. Our interest is focused on the inhibitory properties of berberine and its derivatives in relation to the DNA reparation enzyme Tdp1. Inhibition of various DNA repair enzymes, including Tdp1, is one of the promising fields in the evolution of antitumor therapy.

We synthesized and tested a number of berberine derivatives, which allowed us to identify leading compounds and understand which functional groups should contain a derivative and in which positions in order to exhibit inhibitory activity. According to SAR data analysis only tetrahydroberberine derivatives containing a reduced ring C show inhibitory activity. The presence of substituents in both positions simultaneously (amide or sulfonate group in 9 and bromine in 12) leads to increasing of inhibitory activity (IC50 = 0.5 – 4 µM) in comparison with unbrominated in 12 position analogues.

Almost all of the most active derivatives exhibit low cytotoxicity. Thus, the results reveal that some berberine derivatives are potential agents that can be used in antitumor therapy.
Despite the progress achieved in target, chemo-, and radiotherapy, treatment options for patients with latestage metastatic castration-resistant prostate cancer are presently very limited.Use of dendritic cell-based vaccines exemplified by sipuleucel-T appears is rarely curative and is effective in only a fraction of such patients.Given the success of CAR T cell therapy in the field of B cell malignancies, significant efforts have been made to adapt this powerful technology to the problem of metastatic prostate cancer.

Availability of unique prostate cancer surface targets for CAR T cells has thereby become a pressing issue in the field of CAR design.Ideally, such targets should be absent from normal cells or tissues, be present on all prostate cancer cells across all patients, and be indispensable for the survival of cancer cells.In reality, however, none of the prostate cancer-associated surface markers described to date are matching such description.

Here, we catalogue the list of tested as well as prospective surface antigens to be used as targets for CAR T cell therapy, and discuss the aspects of their safety and potential efficacy.
In this work, mathematical modelling of the process of rupturing the wall of a human fusiform aneurysm is carried out.

We consider 3-parameter and 5-parameter Mooney-Rivlin models. The comparison between the results of the modelling and the experimental data is made. Analyzing the relation of parameters of 3-parameters and 5-parameters Mooney-Rivlin models, the relation between the greatest (least) value and Cauchy strain was found.

The field of application of these models for hemodynamics was shown.