Hengzongshu/ArticleAgent huggingface dataset:
p66shc is a protein product of an mRNA isoform of SHC1 gene that has a pro-oxidant and pro-apoptotic activity and is implicated in the aging process. Mitochondria were suggested as a major source of the p66shc-mediated production of reactive oxygen species (ROS), although the underlying mechanisms are poorly understood.

We studied effects of p66shc on oxidative stress induced by hydrogen peroxide or by serum deprivation in human colon carcinoma cell line RKO and in diploid human dermal fibroblasts (HDFs). An shRNA-mediated knockdown of p66shc suppressed and an overexpression of a recombinant p66shc stimulated the production of ROS in the both models. This effect was not detected in the mitochondrial DNA-depleted ρ0-RKO cells that do not have the mitochondrial electron transport chain (ETC). The p66shc-dependent accumulation of mitochondrial ROS was detected with HyPer-mito, a mitochondria-targeted fluorescent protein sensor for hydrogen peroxide. The fragmentation of mitochondria induced by mitochondrial ROS was significantly reduced in the p66shc deficient RKO cells. Mitochondria-targeted antioxidants SkQ1 and SkQR1 also decreased the oxidative stress induced by hydrogen peroxide or by serum deprivation.

Together the data indicate that the p66shc-dependant ROS production during oxidative stress has mitochondrial origin in human normal and cancer cells.
The paper presents the technique of qualitative assessment of the strength of graphene layers adhesion to the surface of a copper substrate, where they are formed.

The technique uses a complex of approved analytical methods: electron backscatter diffraction (EBSD), Raman spectroscopy and optical microscopy. The technique was tested on multilayer graphene grown on a copper grain with crystal orientation (111).

The presented method can be used to assess the effectiveness of the methods of graphene transfer from grains with different crystal orientation.
well established concepts of adaptive wave front shaping used in imaging through turbid medium

apply well established concepts of adaptive wave front shaping to detect detrimental phase modulated sequences in multi-carrier optical communications

detrimental phase modulated sequences can cause extreme power fluctuations due to dispersion enhanced interference of information symbols
Objective: to study the association of DRD2/ANKK1 Taq1A polymorphism with depression in an open 45–64-year-old male population from Novosibirsk.

Patients and methods. A representative sample of an open 45–64-year-old male population (n=781) was surveyed within Screening IV of the international HAPIEE program and the WHO MONICA-psychosocial program in 2003–2005. All the study participants filled out the WHO MONICA-psychosocial Program Depression Scale. The DRD2/ANKK1 Taq1A C32806T (rs 1800497) polymorphism was genotyped using the published methods within the budgeting topic. The Pearson's chi-square (2) test was applied to test the statistical significance of differences between the groups. Significance in all types of analysis was taken at p ≤0.05.

Results and discussion. The prevalence of depression in the open 45–64-year-old male population was 36.3%: 13.5% of the examinees had severe depression (SD) and 22.8% had moderate depression (MD). A comparative intergroup analysis showed that the odds ratio (OR) for the incidence of SD was 3.86 times higher in the T/C genotype carriers than in the C/C genotype ones, who, on the contrary, had no depression; the OR for the incidence of SD was also 3.28 times higher in the T/C genotype carriers, while MD was more common in the homozygous C/C genotype carriers. The OR for the incidence of SD was 2.63 times higher in the DRD2 T allele carriers than in the C allele carriers who did not suffer from depression in most cases. Conclusion . A significant association was established between the carriage of Taq1A (T allele) and depression in 45–64-year-old males.
The aim of the study was to investigate Klotho protein levels in men with type 2 diabetes blood and its associations with several cardiometabolic risk factors. Material and methods. The study included 37 men with diabetes and 141 men without diabetes. Fasting blood samples were collected to measure Klotho protein levels and some biochemical parameters. Results and its discussion. The Klotho protein level in men with diabetes was significantly lower than in men without diabetes (374 [117; 500] and 515 [315; 1009] pg/dl, p<0.0001). Among the examined men with diabetes with a glomerular filtration rate of less than 60 ml/min/1.73 cm 2 , the concentration of Klotho protein was 4 times lower than in the comparison group (104 [93; 118] and 413 [147; 535] pg/dl, p = 0.014) In men with diabetes, the Klotho protein was inversely correlated with the ratio of waist to hip circumference (-0.329; p = 0.047). But with multivariate analysis, only a tendency towards a negative association of the Klotho protein with abdominal obesity was determined (-0.385, p = 0.078). Conclusion. The content of Klotho protein in men with diabetes is significantly lower, especially in middle-aged men and in those with a reduced glomerular filtration rate. In men with diabetes, the Klotho protein has a negative correlation with the presence of abdominal obesity. In a multivariate analysis among men with diabetes, the Klotho protein tends to be inversely associated with the presence of abdominal obesity.
A comparison of the structure and sensitivity of humidity sensors prepared from graphene (G)-PEDOT: PSS (poly (3,4-ethylenedioxythiophene)) composite material on flexible and solid substrates is performed.

Upon an increase in humidity, the G: PEDOT: PSS composite films ensure a response (a linear increase in resistance versus humidity) up to 220% without restrictions typical of sensors fabricated from PEDOT: PSS. It was found that the response of the examined sensors depends not only on the composition of the layer and on its thickness but, also, on the substrate used. The capability of flexible substrates to absorb the liquid component of the ink used to print the sensors markedly alters the structure of the film, making it more porous; as a result, the response to moisture increases. However, in the case of using paper, a hysteresis of resistance occurs during an increase or decrease of humidity; that hysteresis is associated with the capability of such substrates to absorb moisture and transfer it to the sensing layer of the sensor.

A study of the properties of G: PEDOT: PSS films and test device structures under deformation showed that when the G: PEDOT: PSS films or structures are bent to a bending radius of 3 mm (1.5% strain), the properties of those films and structures remain unchanged. This result makes the composite humidity sensors based on G: PEDOT: PSS films promising devices for use in flexible and printed electronics.
NTRK gene fusions are drivers of tumorigenesis events that specific Trk-inhibitors can target. Current knowledge of the downstream pathways activated has been previously limited to the pathways of regulator proteins phosphorylated directly by Trk receptors.

Here, we aimed to detect genes whose expression is increased in response to the activation of these pathways. We identified and analyzed differentially expressed genes in thyroid cancer samples with NTRK1 or NTRK3 gene fusions, and without any NTRK fusions, versus normal thyroid gland tissues, using data from the Cancer Genome Atlas, the DESeq2 tool, and the Genome Enhancer and geneXplain platforms. Searching for the genes activated only in samples with an NTRK fusion as opposed to those without NTRK fusions, we identified 29 genes involved in nervous system development, including AUTS2, DTNA, ERBB4, FLRT2, FLRT3, RPH3A, and SCN4A. We found that genes regulating the expression of the upregulated genes (i.e., upstream regulators) were enriched in the 'signaling by ERBB4' pathway. Moreover, the algorithm searching for positive feedback loops for gene promoters and transcription factors (a so-called 'walking pathways' algorithm) identified the ErbB4 protein as the key master regulator.

ERBB4 was also one of three genes encoding master regulators whose expression was increased only in samples with an NTRK fusion. ERBB4 upregulation (p-value = 0.004) was confirmed in an independent sample of ETV6-NTRK3-positive FFPE specimens. Thus, ErbB4 is the potential key regulator of the pathways activated by NTRK gene fusions in thyroid cancer. These results are preliminary and require additional biochemical validation.
Primary hyperaldosteronism is a common cause of hypertension.Its prevalence reaches 15% among the forms of arterial hypertension.Prolonged exposure to elevated aldosterone concentrations is associated with target organ damage in the heart, kidney, arterial wall, and high cardiovascular risk in patients with primary hyperaldosteronism.However, the lack of attention to this problem persists among doctors of various specialties, especially general practitioners.Timely diagnosis and treatment of these patients depends on the correct choice of screening and clarifying diagnostic methods.A long ten-year diagnostic way to identify primary hyperaldosteronism in a young person with refractory hypertension is described below.
The importance of the biological properties of proteins to cells cause actively exploring their amino acid composition (primary structure). The versatile tool of cell proteome exploring is mass-spectroscopy. The interpretation of massspectroscopy data is complex challenge because it remains uncertain peptide dissociation mechanisms and external factor influence to peptide fragmentation process. Moreover, a lot of mass-spectroscopy data is required to enhancement existing or development novel algorithms to interpret peptide mass-spectra. The article describes development of algorithm for in silico generation peptide mass-spectra covered the problem of influence noncanonical amino acid composition and posttranslational modifications to dissociation process. Developed algorithm was compared with analogues and evaluated over experimental data.
At present due to depletion of reserves in the majority of Western Siberia oil and gas fields, with reservoirs mainly related to lower Cretaceous and Jurassic section interval, the basement intervals present primary scientific and practical interest for prospecting and exploration.

At the Bystrovka vibroseismic test site (Novosibirsk region), the Paleozoic basement occurs at depth of about 10 m. This allowed investigating its elastic properties with shallow seismic technique using VSP.

Results of these investigations are presented in the present paper.
Functional foods enriched with plant polyphenols and anthocyanins in particular attract special attention due to multiple beneficial bioactive properties of the latter. We evaluated the effects of a grain diet rich in anthocyanins in a mouse model of Alzheimer’s disease induced by amyloid-beta (Aβ) and a transgenic mouse model of Parkinson’s disease (PD) with overexpression of human alpha-synuclein. The mice were kept at a diet that consisted of the wheat grain of near isogenic lines differing in anthocyanin content for five–six months. The anthocyanin-rich diet was safe and possessed positive effects on cognitive function. Anthocyanins prevented deficits in working memory induced by Aβ or a long-term grain mono-diet; they partially reversed episodic memory alterations. Both types of grain diets prolonged memory extinction and rescued its facilitation in the PD model. The dynamics of the extinction in the group fed with the anthocyanin-rich wheat was closer to that in a group of wild-type mice given standard chow. The anthocyanin-rich diet reduced alpha-synuclein accumulation and modulated microglial response in the brain of the transgenic mice including the elevated expression of arginase1 that marks M2 microglia. Thus, anthocyanin-rich wheat is suggested as a promising source of functional nutrition at the early stages of neurodegenerative disorders.
Acute pancreatitis is one of the most common gastrointestinal causes for hospitalization after acute appendicitis. Despite recent advances in the treatment methods, mortality from acute pancreatitis and its complications remains high. Hypertriglyceridemia is an underestimated cause of acute pancreatitis, accounting for 10% among other etiological factors.

Timely diagnosis of hypertriglyceridemia, a personalized approach, and the selection of adequate lipid-lowering therapy allow reducing the risk of developing and/or preventing recurrent acute pancreatitis. The article presents the clinical case of a patient with recurrent pancreatitis and hypertriglyceridemia.

Despite high levels of triglycerides and annual hospitalizations for recurrent pancreatitis, the patient has not received optimal treatment for a long time. Therefore, lipid-modifying therapy has not been prescribed and none dietary recommendations have been suggested, including the exclusion of alcoholic beverages. Structural changes in the pancreas (the formation of pseudocysts) and the progression of pancreatogenic diabetes have developed.
We consider thick domain walls in a de Sitter universe following paper by Basu and Vilenkin. However, we are interested not only in stationary solutions found therein, but also investigate the general case of domain wall evolution with time. When the wall thickness parameter, δ0, is smaller than H−1/√2, where H is the Hubble parameter in de Sitter space-time, then the stationary solutions exist, and initial field configurations tend with time to the stationary ones. However, there are no stationary solutions for δ0 ⩾ H−1/√2. We have calculated numerically the rate of the wall expansion in this case and have found that the width of the wall grows exponentially fast for δ0 ≫ H−1. An explanation for the critical value δ0c = H−1/√2 is also proposed.
We report a study of $B\to (J/\psi \gamma) K$ and $B\to (\psi' \gamma)K$ decay modes using $772\times 10^{6}$ $B\bar{B}$ events collected at the \Upsilon(4S)$ resonance with the Belle detector at the KEKB energy-asymmetric $e^+ e^-$ collider.

We observe $X(3872) \to J/\psi \gamma$ and report the first evidence for $\chi_{c2} \to J/\psi \gamma$ in $B\to (X_{c\bar{c}}\gamma) K$ decays, while in a search for $X(3872) \to \psi' \gamma$ no significant signal is found.

We measure the branching fractions, $\mathcal{B}(B^{\pm} \to X(3872) K^{\pm}) \mathcal{B}(X(3872) \to J/\psi\gamma)$ $=$ $(1.78^{+0.48}_{-0.44}\pm 0.12)\times 10^{-6}$, $\mathcal{B} (B^{\pm} \to\chi_{c2} K^{\pm})$$=$ $(1.11^{+0.36}_{-0.34} \pm 0.09) \times 10^{-5}$, $\mathcal{B}(B^{\pm} \to X(3872) K^{\pm}) \mathcal{B}(X(3872) \to \psi'\gamma)$ $<$ $3.45\times 10^{-6}$ (upper limit at 90% C.L.) and also provide upper limits for other searches.
Metazoan genomes contain many ultra-conserved elements (UCEs), long sequences identical between distant species. In this study we identified UCEs in drosophilid and vertebrate species with a similar level of phylogenetic divergence measured at protein-coding regions, and demonstrated that both the length and number of UCEs are larger in vertebrates. The proportion of non-exonic UCEs declines in distant drosophilids whilst an opposite trend was observed in vertebrates. We generated a set of 2,126 Sophophora UCEs by merging elements identified in several drosophila species and compared these to the eutherian UCEs identified in placental mammals. In contrast to vertebrates, the Sophophora UCEs are depleted around transcription start sites. Analysis of 52,954 P-element, piggyBac and Minos insertions in the D. melanogaster genome revealed depletion of the P-element and piggyBac insertions in and around the Sophophora UCEs. We examined eleven fly strains with transposon insertions into the intergenic UCEs and identified associated phenotypes in five strains. Four insertions behave as recessive lethals, and in one case we observed a suppression of the marker gene within the transgene, presumably by silenced chromatin around the integration site. To confirm the lethality is caused by integration of transposons we performed a phenotype rescue experiment for two stocks and demonstrated that the excision of the transposons from the intergenic UCEs restores viability. Sequencing of DNA after the transposon excision in one fly strain with the restored viability revealed a 47 bp insertion at the original transposon integration site suggesting that the nature of the mutation is important for the appearance of the phenotype. Our results suggest that the UCEs in flies and vertebrates have both common and distinct features, and demonstrate that a significant proportion of intergenic drosophila UCEs are sensitive to disruption.
ЛИЧНОСТНЫЕ ОСОБЕННОСТИ БОЛЬНЫХ АЛКОГОЛИЗМОМ ВТОРОЙ СТАДИИН. Г. Борок (Санкт-Петербург, Россия), М. А. Суботялов (Новосибирск, Россия) В последние годы все чаще вопрос реабилитации больных алкоголизмом сдвигается из медико-биологической в социально-психологическую область.В связи с этим острее встает тема ключевых различий в личности здоровых людей и зависимых, т. к. только опираясь на эти данные, мы можем выстраивать реабилитационные программы.Целью проведенного исследования было выявление личностных особенностей людей с алкогольной аддикцией методами психологической диагностики.Для исследования в экспериментальную группу были отобраны мужчины с диагнозом алкоголизм второй стадии, в контрольную группуздоровые мужчины, не имеющие в анамнезе алкоголизма.В качестве методов диагностики были использованы психологические методики для диагностики уровня субъективного контроля, тревожности, уровня социальной фрустрированности, защитных механизмов и самооценки.Анализ результатов показал ключевые различия между пациентами наркологического диспансера и контрольной выборкой.Значение локуса контроля у больных алкоголизмом ниже на 69 %, чем у здоровых людей.В контрольной выборке выявлен интернальный локус контроля.В диапазон «оптимального» уровня притязаний попадает всего 19 % пациентов, больных алкоголизмом.Эти данные свидетельствует о незрелости личности, неумении ставить реальные цели, нежелании развиваться.Наличие ведущих механизмов защиты сигнализирует об отказе личности от продуктивного разрешения ситуации.У пациентов уровень социальной фрустрированности выше, чем у здоровых людей на 51 %
Dissipative solitons emerge as stable pulse solutions of nonintegrable and nonconservative nonlinear physical systems, owing to a balance of nonlinearity, dispersion, and loss/gain.A considerable research effort has been dedicated to characterizing amplitude and phase evolutions in the spatiotemporal dynamics of dissipative solitons emerging from fiber lasers.Yet, the picture of the buildup process of dissipative solitons in fiber lasers is incomplete in the absence of corresponding information about the polarization evolution.Here, we characterize probabilistic polarization distributions in the buildup of dissipative solitons in a net-normal dispersion fiber laser system, mode-locked by single-wall carbon nanotubes.The output optical spectra under different pump powers are filtered by a tunable filter, and are detected by a polarization state analyzer.The laser system operates from random amplified spontaneous emission into a stable dissipative soliton state as the cavity gain is progressively increased.Correspondingly, the state of polarization of each spectral wavelength converges towards a fixed point.To reveal the invariant polarization relationship among the various wavelength components of the laser output field, the phase diagram of the ellipticity angle and the spherical orientation angle is introduced.We find that, within the central spectral region of the dissipative soliton, the state of polarization evolves with frequency by tracing a uniform arc on the Poincaré sphere, whereas in the edges of the dissipative soliton spectrum, the state of polarization abruptly changes its path.Increasing cavity gain leads to spectral broadening, accompanied by a random scattering of the state of polarization of newly generated frequencies.Further increases of pump power result in dissipative soliton explosions, accompanied by the emergence of a new type of optical polarization rogue waves.These experimental results provide a deeper insight into the transient dynamics of dissipative soliton fiber lasers.
The inverse source problem for 2D advection-diffusion-reaction model with the measurement data in the form of 2D concentration distribution images is considered. These inverse problems arise in the air quality studies with satellite images data avalible. The sensitivity operator composed of the set of the adjoint problems is used to transform the inverse source problem to the quasi-linear operator equation, which depends on the set of the projection basis functions for the incoming measurement data. The algorithm is tested in the scenarion for an atmospheric chemistry transport model.
Speedup and efficiency of two parallel algorithms for calculating the dynamics of the current distribution when the surface of a tungsten sample is heated by an electron beam pulse are presented. The algorithms are implemented using OpenMP technology.

A special case of axial symmetry without taking into account electric driving forces is considered. The temperature in the sample, calculated on the basis of the two-phase Stefan problem, is necessary for solving electrodynamic equations. To implement them, the methods of Jacobi and the successive over-relaxation methods were used. Pipelining is used to parallelize the successive over-relaxation algorithm.

The current is considered as a possible source of rotation of the substance, which is observed in the experiment.
The paper presents a novel three-dimensional quasi-continuous shape sensor based on an FBG array inscribed by femtosecond laser pulses into a 7-core optical fiber with a polyimide protective coating. The measured bending sensitivity of individual FBGs ranges from 0.046 nm/m-1 to 0.049 nm/m-1. It is shown that the sensor allows for reconstructing 2- and 3-dimensional shapes with high accuracy. Due to the high value of the core aperture and individual calibration of each FBG we were able to measure the smallest reported bending radii down to 2.6 mm with a record accuracy of ∼1%. Moreover, we investigate the magnitude of the errors of curves reconstruction and errors associated with measurement of curvature radii in the range from 2.6 to 500 mm. The main factors affecting the accuracy of measurements are also discussed. The temperature resistance of both the inscribed FBG structures and of the protective coating, along with the high mechanical strength of the polyimide, makes it possible to use the sensor in harsh environments or in medical and composite material applications.
High-loading Ni–Mo carbide catalysts were prepared by the modified gel-combustion method under various thermal treatment conditions. All samples were studied by X-ray diffraction (XRD) analysis, which showed that the catalysts could contain cubic and hexagonal molybdenum carbides, nickel, nickel oxide and Ni–Mo solid solutions, depending on the thermal treatment conditions.

Study of catalyst activity and selectivity in the hydrogenation of furfural was carried out in a batch reactor at 150 °C and hydrogen pressure 6.0 MPa. Analysis of the reaction products showed that the highest yields of 2-methylfuran (2-MF) and furfuryl alcohol (FA) were achieved using catalysts synthesized by calcination of the nickel-molybdenum-carbon precursor at 400 °С with the following reduction in a stream of hydrogen at 600 °C.

The best results for production of FA with a yield of 80 mol % and 2-MF with a yield of 29 mol % were observed using Ni6MoC–SiO2 (400/600) and Ni1MoC–SiO2 (400/600) catalysts, respectively. It has been shown that the addition of nickel to the carbide molybdenum catalyst significantly increases the activity of the catalytic systems. In addition, nickel also contributes to the formation of products formed by hydrogenation of the aromatic ring tetrahydrofurfuryl alcohol (THFA) and 2-methyltetrahydrofuran (2-MTHF).