Hengzongshu/ArticleAgent huggingface dataset:
The article presents a historiographical review of the problems of research of ancient ceramics from archaeological sites of East Asia in the chronological interval from 20,000–18,000 to 9,000 years ago. The subject of discussion is the periodization of monuments with early ceramics, the problems of socio-economic conditions of the emergence of pottery technology, its functional role in ancient societies, reconstruction of technological skills and technical levels of pottery, morphology and decor. There are a lot of controversial issues and 'blind spots' in this direction.

The opening of sites with ancient ceramics in East Asia showed that here, on the Pacific (eastern) outskirts of the Eurasian continent, pottery making technology first appeared about 10,000 years earlier than in the Middle East. The invention of ceramics in this region of the world at the turn of the Pleistocene and Holocene should be considered as a result of a combination of natural and social factors.

The article discusses certain regional differences in the formation of skills in making the most ancient ceramic vessels of East Asia. It is assumed that the development of ceramic technology in the Japanese archipelago and in the mainland areas of East Asia took place independently. For the Amur region, there are two local cultural traditions – Osipovskaya and Gromatukhinskaya. According to materials from Northern China, there is a version of the existence of a common line in the development of ancient ceramics in the Valley of the Nonny River. There are similarities between the early ceramics of Northeast China, and the Gromatukhinskaya and Osipovskaya cultures of Amur. For the ceramics of South Korea also note similarities with the materials of the Russian Far East, Japanese and Chinese dishes.
Background DNA repair is essential to counteract damage to DNA induced by endo- and exogenous factors, to maintain genome stability. However, challenges to the faithful discrimination between damaged and non-damaged DNA strands do exist, such as mismatched pairs between two regular bases resulting from spontaneous deamination of 5-methylcytosine or DNA polymerase errors during replication. To counteract these mutagenic threats to genome stability, cells evolved the mismatch-specific DNA glycosylases that can recognize and remove regular DNA bases in the mismatched DNA duplexes. The Escherichia coli adenine-DNA glycosylase (MutY/MicA) protects cells against oxidative stress-induced mutagenesis by removing adenine which is mispaired with 7,8-dihydro-8-oxoguanine (8oxoG) in the base excision repair pathway. However, MutY does not discriminate between template and newly synthesized DNA strands. Therefore the ability to remove A from 8oxoG•A mispair, which is generated via misincorporation of an 8-oxo-2′-deoxyguanosine-5′-triphosphate precursor during DNA replication and in which A is the template base, can induce A•T→C•G transversions. Furthermore, it has been demonstrated that human MUTYH, homologous to the bacterial MutY, might be involved in the aberrant processing of ultraviolet (UV) induced DNA damage.

Methods Here, we investigated the role of MutY in UV-induced mutagenesis in E. coli . MutY was probed on DNA duplexes containing cyclobutane pyrimidine dimers (CPD) and pyrimidine (6–4) pyrimidone photoproduct (6–4PP). UV irradiation of E. coli induces Save Our Souls (SOS) response characterized by increased production of DNA repair enzymes and mutagenesis. To study the role of MutY in vivo, the mutation frequencies to rifampicin-resistant (Rif R ) after UV irradiation of wild type and mutant E. coli strains were measured.

Results We demonstrated that MutY does not excise Adenine when it is paired with CPD and 6–4PP adducts in duplex DNA. At the same time, MutY excises Adenine in A•G and A•8oxoG mispairs. Interestingly, E. coli mutY strains, which have elevated spontaneous mutation rate, exhibited low mutational induction after UV exposure as compared to MutY-proficient strains. However, sequence analysis of Rif R mutants revealed that the frequencies of C→T transitions dramatically increased after UV irradiation in both MutY-proficient and -deficient E. coli strains. Discussion These findings indicate that the bacterial MutY is not involved in the aberrant DNA repair of UV-induced DNA damage.
Cosmic ray data collected by the KASCADE air shower experiment are competitive in terms of quality and statistics with those of modern observatories.

We present a novel mass composition analysis based on archival data acquired from 1998 to 2013 provided by the KASCADE Cosmic ray Data Center (KCDC). The analysis is based on modern machine learning techniques trained on simulation data provided by KCDC.

We present spectra for individual groups of primary nuclei, the results of a search for anisotropies in the event arrival directions taking mass composition into account, and search for gamma-ray candidates in the PeV energy domain.
Drosophila protein GAGA (GAF) is a factor of epigenetic transcription regulation of a large group of genes with a wide variety of cellular functions. GAF is encoded by the Trithorax-like (Trl) gene, which is important for the formation of various organs and tissues at all stages of ontogenesis. In our previous works, we showed that this protein is necessary for the development of the reproductive system, both in males and females of Drosophila. Decreased expression of the Trl gene led to multiple disorders of spermatogenesis and oogenesis. One of the significant disorders was associated with massive degradation and loss of cells in the germline.

In this work, we carried out a more detailed cytological study to determine what type of germ cell death is characteristic of Trl mutants, and whether there are disturbances or changes in this process compared to the norm.

The results obtained showed that the lack of GAF protein causes massive germ cell death in both females and males of Drosophila, but this death manifests itself in different ways, depending on the sex. In Trl females, this process does not differ phenotypically from the norm. In the dying egg chambers, signs of apoptosis and autophagy were revealed, as well as morphological features that are characteristic of the wild type. In males, Trl mutations induce mass germ cell death through autophagy, which is not typical of Drosophila spermatogenesis, and has not been previously described, neither in the norm nor in other genes' mutations. Thus, GAF lack in Trl mutants leads to increased germ cell death through apoptosis and autophagy. Ectopic cell death and germ line atrophy are probably associated with impaired expression of the GAGA factor target genes, among which there are genes that regulate both apoptosis and autophagy.
To date biomedicine and pharmacology have required generating new and more consummate models. One of the most perspective trends in this field is using induced pluripotent stem cells (iPSCs). iPSC application requires careful high-throughput analysis at the molecular, epigenetic, and functional levels. The methods used have revealed that the expression pattern of genes and microRNA, DNA methylation, as well as the set and pattern of covalent histone modifications in iPSCs, are very similar to those in embryonic stem cells. Nevertheless, iPSCs have been shown to possess some specific features that can be acquired during the reprogramming process or are remnants of epigenomes and transcriptomes of the donor tissue. These residual signatures of epigenomes and transcriptomes of the somatic tissue of origin were termed "epigenetic memory." In this review, we discuss the "epigenetic memory" phenomenon in the context of the reprogramming process, its influence on iPSC properties, and the possibilities of its application in cell technologies.
Formic acid decomposition to H2-rich gas was investigated over a CuO-CeO2/γ-Al2O3 catalyst.

The catalyst was characterized by XRD, HR TEM and EDX methods. A 100% conversion of formic acid was observed over the copper-ceria catalyst under ambient pressure, at 200–300 °C, N2:HCOOH = 75:25 vol.% and flow rate 3500–35,000 h−1 with H2 yield of 98%, wherein outlet CO concentration is below the equilibrium data (<0.5 vol.%).

The copper-ceria catalyst proved to be promising for multifuel processor application, and the H2 generation from dimethoxymethane, methanol, dimethyl ether and formic acid on the same catalyst for fuel cell supply.
It is shown that the mechanism of the gas molecules transfer through the lipid bilayer can be connected with the kink-solitons. They are appeared in the lipid bilayer as a result of structural changes in biomembranes.

The analytical model describing the behavior of kink-solitons in the finite hydrocarbon chain is presented. The kink-solitons are formed during the folding of the erythrocyte as it moves along the microcapillaries. The equation of the kink-soliton motion is derived. This equation is numerically solved.

It is shown that kink-solitons can appear in both saturated and unsaturated alkyl chains. In unsaturated chains, the soliton is "smeared out," CH2−groups vibrate around C=C bonds with a significant amplitude, taking away part of the soliton's energy. The transport of gas molecules by kink-solitons in unsaturated chains is hindered in comparison with saturated hydrocarbon chains. When the mechanical stress field changes, a sharp change in membrane permeability occurs.
It has long been known that defects in the structure of the mitochondrial genome can cause various neuromuscular and neurodegenerative diseases. Nevertheless, at present there is no effective method for treating mitochondrial diseases. The major problem with the treatment of such diseases is associated with mitochondrial DNA (mtDNA) heteroplasmy. It means that due to a high copy number of the mitochondrial genome, mutant copies of mtDNA coexist with wild-type molecules in the same organelle. The clinical symptoms of mitochondrial diseases and the degree of their manifestation directly depend on the number of mutant mtDNA molecules in the cell. The possible way to reduce adverse effects of the mutation is by shifting the level of heteroplasmy towards the wild-type mtDNA molecules. Using this idea, several gene therapeutic approaches based on TALE and ZF nucleases have been developed for this purpose. However, the construction of protein domains of such systems is rather long and laborious process.

Meanwhile, the CRISPR/Cas9 system is fundamentally different from protein systems in that it is easy to use, highly efficiency and has a different mechanism of action. All the characteristics and capabilities of the CRISPR/Cas9 system make it a promising tool in mitochondrial genetic engineering. In this article, we demonstrate for the first time that the modification of gRNA by integration of specific mitochondrial import determinants in the gRNA scaffold does not affect the activity of the gRNA/Cas9 complex in vitro.

All the characteristics and capabilities of the CRISPR/Cas9 system make it a promising tool in mitochondrial genetic engineering. In this article, we demonstrate for the first time that the modification of gRNA by integration of specific mitochondrial import determinants in the gRNA scaffold does not affect the activity of the gRNA/Cas9 complex in vitro.
The article is devoted to the choice of a clear and concise form for presenting the results of analise and comparing programming languages and systems, convenient for assessing the expressive power of languages and the complexity of implementing systems. Many works on methods for developing software systems depend on the practicality of approaches to decomposition of programs debugged using programming systems.

The formalization is adapted to the paradigm analysis of the definitions of programming languages and the selection of practical criteria for decomposition of programs. The semantic decomposition of the definitions of languages and systems as part of the analysis of programming paradigms was chosen as the main ap-proach.

Such a choice makes it possible to single out autonomously developed typical program components that can be adapted to the design of various information systems. The solution to this problem is useful when studying programming methods, studying the history of programming languages, for comparing programming paradigms, the potential of used circuits and models, assessing the novelty level of created programming languages, and also when choosing criteria for program decomposition. In addition, their existence allows us to form a teaching methodology for developing the components of information systems. Along the way, the distance in conceptual complexity between programming and programming system development is shown.
Paleontologists define global extinctions on Earth as a loss of about three-quarters of plant and animal species over a relatively short period of time. At least five global extinctions are documented in the Phanerozoic fossil record (~500-million-year period): ~65, 200, 260, 380, and 440 million years ago. In addition, there is evidence of global extinctions in earlier periods of life on Earth – during the Late Cambrian (~500 million years ago) and Ediacaran periods (more than 540 million years ago). There is still no common opinion on the causes of their occurrence.

The current study is a systematized review of the data on recorded extinctions of complex life forms on Earth from the moment of their occurrence during the Ediacaran period to the modern period. The review discusses possible causes for mass extinctions in the light of the influence of abiogenic factors, planetary or astronomical, and the consequences of their actions. We evaluate the pros and cons of the hypothesis on the presence of periodicity in the extinction of Phanerozoic marine biota.

Strong evidence that allows us to hypothesize that additional mechanisms associated with various internal biotic factors are responsible for the emergence of extinctions in the evolution of complex life forms is discussed. Developing the idea of the internal causes of periodicity and discontinuity in evolution, we propose our own original hypothesis, according to which the bistability phenomenon underlies the complex dynamics of the biota development, which is manifested in the form of global extinctions. The bistability phenomenon arises only in ecosystems with predominant sexual reproduction. Our hypothesis suggests that even in the absence of global abiotic catastrophes, extinctions of biota would occur anyway. However, our hypothesis does not exclude the possibility that in different periods of the Earth’s history the biota was subjected to powerful external influences that had a significant impact on its further development, which is reflected in the Earth’s fossil record.
It also discusses the term 'compound verb' and its understanding in Russian and foreign linguistics.

This article analyzes compound verbs of modern Japanese language belonging to the reciprocal and joint action group expressing quantitative meaning. The compound verb is a verb in which from the left side a nominal, verbal or adjective component may be added to a verb functioning as a second component of the compound verb, from the right side – the auxiliary verb suru 'to do'. We suppose that the compound verb is a verb combining two verbal components. The first component has the form of a connective form of the verb, and the second one is a verb having a full word inflectional paradigm.

Components 合う -au and 合わせる -awaseru are used as affixes in analyzed compound verbs. Compound verbs with reciprocal meaning with component 合う -au express subjective reciprocal meaning and suppose existence of two participants of action. Compound verbs with joint action meaning with component 合う -au can express an indefinite quantity of subjects of action with the help of using adverbs or the appropriate lexicon as the subject of a sentence. The second component 合う -au easily adjoins to the verbs implying existence of a partner and doesn't join to verbs which have a strong meaning of individuality and separateness. The second component 合わせる -awaseru partially keeps its own lexical meaning and the compound verb overall has an objective reciprocal meaning and expresses the quantity of subjects or objects. Compound verbs with component 合わせる -awaseru have the following meanings: 1. Combination of two subjects or two items. In this group as a first component the verbs with meaning 'joint, adding' and verbs with meaning 'sudden joint action of partners' are often used. 2. Adding one subject to another, one item to another. Compound verb overall expresses meaning of the result of action. 3. Adaptation to some circumstances, comparison. Conclusion is made based on the comparison of some items.
The studies under extreme conditions offer insight into the fine structural changes that occur as a function of temperature or pressure variations.Molecular crystals are of special interest both from fundamental and applied point of view.Many of them are promising as pharmaceuticals, materials for electronics, optics and soft robotics.Despite new developments in high-pressure instrumentation and diffraction techniques, the quality of diffraction data and a way of performing diffraction experiment still remain critically important for obtaining reliable information on crystal structure behavior at high pressures.

In this contribution several examples of single-crystal studies at high pressures on laboratory and synchrotron facilities will be discussed.In each of the examples pressure variation protocol, data collection time, hardware and facility type influenced strongly not only the quality and completeness of experimental data, but even the crystal structures of resulting high pressure phases.

Advantages and disadvantages of different instrumentation will also be discussed along with information which high-pressure single-crystal X-ray diffraction can provide for studying solid state processes.
Рассматривается использование моделей аргументации в автороведческой экспертизе.

Выдвигается гипотеза, согласно которой автор в сходных коммуникативных ситуациях применяет одну и ту же структуру аргументативного высказывания, модель аргументации, при
We search for bottomonium states in ϒ(2S) → (b b)γ decays using 158 × 10 6 ϒ(2S) events collected with the Belle detector at the KEKB e + e -collider.

The (b b) system is reconstructed in 26 exclusive hadronic final states composed of charged pions, kaons, protons, and K 0 S mesons.

We find no evidence for the state recently observed around 9975 MeV (X b b) in an analysis based on a data sample of 9.3 × 10 6 ϒ(2S) events collected with the CLEO III detector. We set a 90% confidence level upper limit on the branching fraction. This result is an order of magnitude smaller than the measurement reported with CLEO data. We also set an upper limit for the η (iP)] < 3.7 × 10 -6 .
Triarylmethyl (TAM) radicals are widely used in Electron Paramagnetic Resonance (EPR) spectroscopy as spin labels and in EPR imaging as spin probes for in vivo oxymetry. One of the key advantages of TAMs is extremely narrow EPR line, especially in case of deuterated analogues (~5 μT). Another advantage is their slow spin relaxation even at physiological temperatures allowing, in particular, application of pulsed dipolar EPR methods for distance measurements in biomolecules.

In this paper a large series of TAM radicals and their deuterated analogues is synthesized, and corresponding spectroscopic parameters including 13C hyperfine constants are obtained for the first time.

The negligible dependence of 13C hyperfine constants on solvent, as well as on structure and number of substituents at para-C atoms of aromatic rings, has been found. In addition, we have demonstrated that 13C signals at natural abundance can be employed for successful room-temperature distance measurements using Pulsed Electron Double Resonance (PELDOR or DEER).
For the first time, two types of hybrid cells with embryonic stem (ES) cell-like and fibroblast-like phenotypes were produced through the fusion of mouse ES cells with fibroblasts.

Transcriptome analysis of 2,848 genes differentially expressed in the parental cells demonstrated that 34-43% of these genes are expressed in hybrid cells, consistent with their phenotypes; 25-29% of these genes display intermediate levels of expression, and 12-16% of these genes maintained expression at the parental cell level, inconsistent with the phenotype of the hybrid cell. Approximately 20% of the analyzed genes displayed unexpected expression patterns that differ from both parents. An unusual phenomenon was observed, namely, the illegitimate activation of Xist expression and the inactivation of one of two X-chromosomes in the near-tetraploid fibroblast-like hybrid cells, whereas both Xs were active before and after in vitro differentiation of the ES cell-like hybrid cells.

These results and previous data obtained on heterokaryons suggest that the appearance of hybrid cells with a fibroblast-like phenotype reflects the reprogramming, rather than the induced differentiation, of the ES cell genome under the influence of a somatic partner.
We observed generation of stable picoseconds pulse train and double-scale optical lumps with picosecond envelope and femtosecond noise-like oscillations in the same Yb-doped fiber laser with all-positive-dispersion cavity mode-locked due to the effect of non-linear polarization evolution.

In the noise-like pulse generation regime the auto-correlation function has a non-usual double (femto- and picosecond) scale shape. We discuss mechanisms of laser switching between two operation regimes and demonstrate a good qualitative agreement between experimental results and numerical modeling based on modified nonlinear Schrödinger equations.

We discuss mechanisms of laser switching between two operation regimes and demonstrate a good qualitative agreement between experimental results and numerical modeling based on modified nonlinear Schrödinger equations.
The present study examines the effects of serotonin (5-HT) 1A receptor ligands on humoral im-mune response in two rat lines selected for over 75 generations for the enhancement or elimination of aggression. Activation of presynaptic 5-HT1A receptors with a low dose of the selective 5-HT1A receptor agonist 8-OH-DPAT (0.1 mg/kg) or the blockade of postsynaptic 5-HT1A receptors with the antagonist WAY-100635 (1.0 mg/kg) did not affect the numbers of IgM-antibody forming cells (IgM-AFC) in the spleen of highly aggressive rats, which were characterized by higher immune responsiveness compared to nonaggressive line. On the other hand, the same doses of 8-OH-DPAT and WAY-100635, as well as a higher dose of 8-OH-DPAT (1.0 mg/kg), which is known to activate postsynaptic 5-HT1A receptors, produce immunostimulation in nonaggressive rats. However, only the highest dose of 8-OH-DPAT (5.0 mg/kg) was able to cause immunosuppression in nonaggressive rats that was mainly dependent on stimulation of postsynaptic 5-HT1A receptors. In contrast to nonaggressive rats, the dose of 1.0 mg/kg 8-OH-DPAT was sufficient to produce a decrease in the numbers of IgM-AFC in highly aggressive rats. Thus, pharmacological activation of pre- and postsynaptic 5-HT1A receptors, as well as the blockade of postsynaptic 5-HT1A receptors, produced different effects on the immune response in two lines of rats selected for high level of aggression or its absence. These data may have implications for more efficient treatments of a number of mental disorders associated with abnormal aggression.
Direct model-independent relation between the secular perihelion precession of a planet and the density of dark matter ρ dm at its orbit is indicated, and used to deduce upper limits on local values of ρ dm .
Some issues of aerodynamics of a vehicle moving in a vacuum transportation system are formulated and considered. Expected values of aerodynamic forces are estimated in wide ranges of vacuum pressures and velocities of vehicle motion. A problem of gas-dynamic starting of a pipeline-central body configuration is formulated.