Hengzongshu/ArticleAgent huggingface dataset:
The antioxidant system (AOS) maintains the optimal concentration of reactive oxygen species (ROS) in a cell and protects it against oxidative stress. In plants, the AOS consists of seven main classes of antioxidant enzymes, low-molecular antioxidants (e.g., ascorbate, glutathione, and their oxidized forms) and thioredoxin/glutaredoxin systems which can serve as reducing agents for antioxidant enzymes.

We focused this review on the existing scientific background and experimental data used for the systems biology research of the plant AOS.

The number of genes encoding AOS enzymes varies between classes, and same class enzymes encoded by different gene copies may have different subcellular localizations, functional loads and modes of evolution. These facts hereafter reinforce the complex nature of AOS regulation and functioning. Further studies can describe new trends in the behavior and functioning of systems components, and provide new fundamental knowledge about systems regulation. The system is revealed to have a lot of interactions and interplay pathways between its components at the subcellular level (antioxidants, enzymes, ROS level, and hormonal and transcriptional regulation). These facts should be taken into account in further studies during the AOS modeling by describing the main pathways of generating and utilizing ROS, as well as the associated signaling processes and regulation of the system on cellular and organelle levels, which is a complicated and ambitious task. Another objective for studying the phenomenon of the AOS is related to the influence of cell dynamics and circadian rhythms on it. Therefore, the AOS requires an integrated and multi-level approach to study.
The isolation and study of autologous human stem cells remain among the most urgent problems in cell biology and biomedicine to date.

Induced pluripotent stem cells can be derived from human somatic cells by the overexpression of a number of genes. In this study we reprogrammed fetal human skin fibroblasts by transduction with retroviral vectors carrying murine Oct4 , Sox2 , Klf4 , and c-Myc cDNAs.

As a result, cells with the protein expression and gene transcription pattern characteristic of human embryonic stem cells were derived. These induced pluripotent cells are capable of differentiation in vitro into the ectoderm, mesoderm, and endoderm derivatives.
Human 8-oxoguanine-DNA glycosylase (OGG1) efficiently removes mutagenic 8-oxo-7,8-dihydroguanine (8-oxoGua) and 2,6-diamino-4-hydroxy-5-formamidopyrimidine when paired with cytosine in oxidatively damaged DNA. Excision of 8-oxoGua mispaired with adenine may lead to G-->T transversions. Post-translational modifications such as phosphorylation could affect the cellular distribution and enzymatic activity of OGG1. Mutations and polymorphisms of OGG1 may affect the enzymatic activity and have been associated with increased risk of several cancers. In this study, we used double-stranded oligodeoxynucleotides containing 8-oxoGua:Cyt or 8-oxoGua:Ade pairs, as well as gamma-irradiated calf thymus DNA, to investigate the kinetics and substrate specificity of several known OGG1 polymorphic variants and phosphomimetic Ser-->Glu mutants. Among the polymorphic variants, A288V and S326C displayed opposite-base specificity similar to that of wild-type OGG1, whereas OGG1-D322N was 2.3-fold more specific for the correct opposite base than the wild-type enzyme. All phosphomimetic mutants displayed approximately 1.5-3-fold lower ability to remove 8-oxoGua in both assays, whereas the substrate specificity of the phosphomimetic mutants was similar to that of the wild-type enzyme. OGG1-S326C efficiently excised 8-oxoGua from oligodeoxynucleotides and 2,6-diamino-4-hydroxy-5-formamidopyrimidine from gamma-irradiated DNA, but excised 8-oxoG rather inefficiently from gamma-irradiated DNA. Otherwise, kcat values for 8-oxoGua excision obtained from both types of experiments were similar for all OGG1 variants studied. It is known that the human AP endonuclease APEX1 can stimulate OGG1 activity by increasing its turnover rate. However, when wild-type OGG1 was replaced by one of the phosphomimetic mutants, very little stimulation of 8-oxoGua removal was observed in the presence of APEX1.
We propose a method for characterization of mature red blood cells (RBCs) morphology, based on measurement of light-scattering patterns (LSPs) of individual RBCs with the scanning flow cytometer and on solution of the inverse light-scattering (ILS) problem for each LSP. We considered a RBC shape model, corresponding to the minimal bending energy of the membrane with isotropic elasticity, and constructed an analytical approximation, which allows rapid simulation of the shape, given the diameter and minimal and maximal thicknesses. The ILS problem was solved by the nearest-neighbor interpolation using a preliminary calculated database of 250,000 theoretical LSPs. For each RBC in blood sample we determined three abovementioned shape characteristics and refractive index, which also allows us to calculate volume, surface area, sphericity index, spontaneous curvature, hemoglobin concentration and content.
The Ulan-Sar’dag mélange comprises of a seafloor and island-arc system of remnants of the Paleo-Asian Ocean. The Ulan-Sar’dag mélange includes blocks of mantle peridotite, podiform chromitite, cumulate rocks, deep-water siliceous chert, and metavolcanic rocks of the Ilchir suite. The ophiolitic unit shows overturned pseudostratigraphy. The nappe of mantle tectonites is thrusted over the volcanic-sedimentary sequence of the Ilchir suite.

We present new whole-rock major and trace element, mineral chemistry, and U-Pb isotope data for the Ulan-Sar’dag mélange, including different lithostratigraphic units: Ophiolitic, mafic rocks and metavolcanites. Detailed studies on the magmatic rocks led to the discovery of a rock association that possesses differing geochemical signatures within the studied area. U–Pb dating of zircons from the trachyandesite, belonging to the second geochemical type, yielded a date of 833 ± 4 Ma.

The mantle peridotite and cumulate rocks formed in a supra-subduction zone environment. The mafic and metavolcanic rocks belong to the following geochemical types: (1) Ensimatic island-arc boninites; (2) island-arc calc-alkaline andesitic basalts, andesites, and dacites; (3) tholeiitic basalts of mid-ocean ridges; and (4) oceanic island basalts. The date of 833 ± 4 Ma is interpreted as the crystallization age during mature island-arc and intra-arc rifting stages. The possible influence of later plume magmatic-hydrothermal activities led to the appearance of moderately alkaline igneous rocks with a significant subduction geochemical fingerprint.
The results of experimental studies of local MHD interaction near bodies of various configurations are presented in the case when the work of the volumetric electromagnetic force leads to the deceleration of the hypersonic air flow, to the fixation of the ionization region in the flow, to the change of pressure in the interaction zone and to the appearance of a bow shock wave in front of the interaction zone. Shown, that at strong MHD-interaction the shape of the model slightly influences the final result of the change in the flow pattern, since the size of the interaction region becomes comparable, and in some cases larger than the size of the streamlined body.
Background: DNA repair is a vitally important process that protects the cell's genome from ongoing damage.Six DNA repair pathways are commonly outlined: direct reversal, base excision repair, nucleotide excision repair, mismatch repair, recombination repair, and non-homologous end joining.Of these, base excision repair that deals with small non-bulky lesions removes the greatest number of damaged nucleotides daily.Unlike many other cellular pathways, base excision repair is not carried out by multienzyme complexes but rather involves many DNA-mediated transient protein-protein interactions that pass the damaged DNA between consecutive participants of the pathway.Methods: Direct structural characterization of such DNA substrate channeling remains elusive, and most of what is known about it was obtained through enzyme kinetics and modeling.Results: DNA glycosylases, the enzymes that initiate base excision repair, are actively displaced from their enzyme-product complexes by abasic site (AP) endonucleases, the next enzyme in the pathway.Several accessory proteins, such as XRCC1, PCNA, and PARP1, guide the dynamic assembly and disassembly of DNA repair complexes providing regulatory modifications and scaffolding.Interestingly, eukaryotic and bacterial DNA repair proteins are often homologous but the eukaryotic proteins carry additional extended intrinsically disordered domains, which are believed either to be involved into dynamic protein-protein interactions or provide additional DNA-binding capabilities outside the welldefined DNA binding domains.Conclusion: Overall, DNA repair, and especially base excision DNA repair, relies on extensive conformational rearrangements of the participating proteins, which is necessary both for damage recognition and for progress along the repair pathway.As such, they represent a promising target for studies with cryo-EM techniques.
The paper presents a two-dimensional model of elastic deformations. An isotropic medium region in near a crack propagating along the surface is considered.

The results correlate well with an analytical solution.
The research is devoted to the scientific justification of metal processing by pressure with the help of thick monolithic plates forming (thickness 40 mm) from the V95 (analog 7475) (Al-Zn-Mg-Cu) and V-1461 (analog 2099) (Al-Cu-Li-Zn) alloys in creep and close-to-superplasticity. Optimum parameters of the technological process of plate forming are described. The effect of temperature on the magnitude of mechanical stresses (relaxation) during the tests of materials on pure bending is experimentally determined. Forming of thick plates (40 mm) on the UFP-1M unit, and the control of the obtained surface, in comparison with the given electronic model, made it possible to experimentally determine the time and number of forming stages. Mechanical properties of the material after the technological process and heat treatment are preliminary evaluated. The efficiency of using the obtained parameters of the technological process and treatment of metals by pressure in such methods in general is shown.
The experiments on obtaining gas hydrate of refrigerant 134a were carried out by the method, based on explosive boiling-up of a layer of liquefied gas in a bulk of water at decompression.

It is shown that this method combines several factors, leading to intensification of hydrate formation process, resulting in the fast gas hydrate growth.

The effect of the decompression rate on the volume of produced hydrate was studied experimentally.
solving the bending problems of laminated composites

A new beam multilayer finite element is proposed. The finite element model is based on the asymptotic splitting method, which allows one to obtain a solution to the linear spatial problem of the theory of elasticity without using simplifying hypotheses

The advantage of this approach is that all components of the stress tensor are taken into account and that the stress states of composite beams of a complex cross-section are examined in detail without using the plane strain or plane stress states
The article is devoted to the overview of the most popular digital repository support systems and their information model.

The choice of DSpace for the data warehouse of the scientific and educational information system is justified.
Substitution of the halide ligands in (Bu4N)2[Mo3S7X6] (X = Cl, Br) by diimine ligands, such as 4,4'-dimethyl-2,2'-bipyridine (dmbpy), 2,2'-bipyridine (bpy) and 1,10-phenanthroline (phen), affords the neutral heteroleptic clusters Mo3S7Cl4(dmbpy) (), Mo3S7Br4(dmbpy) (), Mo3S7Br4(bpy) (), and Mo3S7Br4(phen) (). Further substitution of the halide ligands in Mo3S7Br4(diimine) clusters by dmit (1,3-dithiole-2-thione-4,5-dithiolate) allows the preparation of the mixed diimine-dithiolene neutral cluster complexes Mo3S7(dnbpy)(dmit)2 (, dnbpy = 4,4'-dinonyl-2,2'-bipyridine), Mo3S7(dcmbpy)(dmit)2 (, dcmbpy = 4,4'-dimethoxycarbonyl-2,2'-bipyridine), and Mo3S7(dcbpy)(dmit)2 (, dcbpy = 2,2'-bipyridine-4,4'-dicarboxylic acid).

The optical limiting properties of complexes have been assessed by the open-aperture Z-scan technique at 570 nm, employing a nanosecond optical parametric oscillator. In order to investigate the effect of increasing the π-system, complexes , with the general formula Mo3S7X4(diimine), (X = Cl, Br), were compared to clusters , containing the dmit ligand. The influence of the metal content on the optical power limiting properties was also investigated by comparing the trinuclear series of complexes prepared herein with the bis(dithiolene) dinuclear cluster (Et4N)2[Mo2O2S2(BPyDTS2)2], which has been recently prepared by our group.

All trinuclear clusters are efficient optical limiters (σeff > σ0) with the threshold limiting fluence F15% decreasing on proceeding from dinuclear to trinuclear clusters and, generally, on extending the π-system.
The model of a thermochemical mantle plume is described. The scheme of origination of the plume from the core-mantle boundary is presented. The basic ratios for determining the thermal power and the diameters of thermochemical plumes are given. After eruption of the melt from the plume conduit to the surface, melting occurs along the base of the crustal block above the plume roof, resulting in the formation of a mushroom-shaped head of the plume, which means that a large intrusive body (deep-rooted batholith) is formed. The relative thermal power of such plumes is 1.9<Ka<10. Based on the laboratory and theoretical modeling results, we present the thermal and hydrodynamic structure of the thermochemical plume with the mushroom-shaped head. The parameters of some plumes, that are responsible for formations of batholiths in North Asia, are estimated from the geological data, including the age intervals and the extent of magmatism. Relying on the model of the flat horizontal liquid layer, hydrodynamics and heat transfer of the mushroom-shaped plume head are considered. The variations in temperature and flow velocity in the melt of the plume head are assessed. The compositional changes in the melt of the plume head are determined by stages: (1) after settling of refractory minerals; (2) after settling of plagioclase in the melt resulting from the first stage. The tables show the calculation data, including the weight contents of oxides and the normative compositions for the melts at T melt =1410 °C and T melt =1380 °C. The thickness of the residual melt is estimated for the case of the Khentei plume. Its head’s thickness ( l ) is equal to the plume conduit diameter ( d ): l = d =29 km. The proposed model of the plume with the mushroom-shaped head was used to calculate the normative composition of the melt with a chemical composition similar to that of normal granites.
Early-life stress affects neuronal plasticity of the brain regions participating in the implementation of social behavior. Our previous studies have shown that brief and prolonged separation of pups from their mothers leads to enhanced social behavior in adult female mice. The goal of the present study was to characterize the expression of genes (which are engaged in synaptic plasticity) Egr1, Npas4, Arc, and Homer1 in the prefrontal cortex and dorsal hippocampus of adult female mice with a history of early-life stress. In addition, we evaluated the expression of stress-related genes: glucocorticoid and mineralocorticoid receptors (Nr3c1 and Nr3c2) and Nr1d1, which encodes a transcription factor (also known as REVERBα) modulating sociability and anxiety-related behavior. C57Bl/6 mice were exposed to either maternal separation (MS, 3 h once a day) or handling (HD, 15 min once a day) on postnatal days 2 through 14. In adulthood, the behavior of female mice was analyzed by some behavioral tests, and on the day after the testing of social behavior, we measured the gene expression. We found increased Npas4 expression only in the prefrontal cortex and higher Nr1d1 expression in both the prefrontal cortex and dorsal hippocampus of adult female mice with a history of MS. The expression of the studied genes did not change in HD female mice. The expression of stress-related genes Nr3c1 and Nr3c2 was unaltered in both groups. We propose that the upregulation of Npas4 and Nr1d1 in females with a history of early-life stress and the corresponding enhancement of social behavior may be regarded as an adaptation mechanism reversing possible aberrations caused by early-life stress.
In the T(1;2)dorvar7 translocation, the 1A-2B7-8 segment of the X chromosome is brought to the vicinity of 2R-chromosome heterochromatin resulting in position effect variegation of dor, BR-C and more distal genes, as well as compaction of chromatin in this segment. By irradiation of T(1;2)dorvar7, nine reversions (rev) to a normal phenotype were recovered. In two cases (rev27, rev226), the 1A-2B7-8 section is relocated to the 19A region of the X chromosome, forming free duplications (1A-2B7-8/19A-20F-X-het). Modifiers of position effect do not change the normal expression of the BR-C and dor genes in these duplications. In five reversions (rev3, rev40, rev60, rev167, rev175), free duplications have formed from the 1A-2B7-8 fragment and X chromosome heterochromatin. In these rearrangements, modifiers of position effect (low temperature, removal of Y and 2R-chromosome heterochromatin and a genetic enhancer (E-var(3)201) induce position-effect again. Two reversions (rev45 and rev110) are associated with additional inversions in the original dorvar7 chromosomes. The inversions relocate part of the heterochromatin adjacent to the 1A-2B7-8 section into new positions. In T(1;2)dorrev45, position-effect is seen in the 2B7-8-7A element as compaction spreading from 2B7-8 proximally in some cases as far as the 5D region. Thus, in rev45 the pattern of euchromatin compaction is reciprocal to that of the initial dorvar7 strain. Apparently, it is due to the same variegation-evoking center near the 2R centromere in both cases. In all nine revertants, weakening or complete disappearance of the position-effect is observed despite retention of the 20-kb heterochromatic segment adjacent to the 1A-2B7-8 region. Thus, a 20-kb heterochromatic sequence does not inactivate euchromatin joined to it.
Large data amount, about 1 ab −1 of integrated luminosity, collected in experiments with the Belle detector provided a good possibilities for precise measurements of the hadronic cross sections in e + e − annihilation.

Main results obtained in this field with the Belle detector

perspectives of the new experiments with the Belle II detector at the SuperKEKB collider
Time-resolved dynamics of the photodissociation of molecular oxygen, O2, via the Σu−3 ion-pair state have been studied with femtosecond time resolution using a pump-probe scheme in combination with velocity map imaging of the resulting O+ and O− ions. The fourth harmonic of a femtosecond titanium-sapphire (Ti:sapphire) laser (λ≈205nm) was found to cause three-photon pumping of O2 to a level at 18.1eV. The parallel character of the observed O+ and O− images allowed us to conclude that dissociation takes place on the Σu−3 ion-pair state. The 815nm fundamental of the Ti:sapphire laser used as probe was found to cause two-photon electron photodetachment starting from the O2 ion-pair state, giving rise to (O(P3)+O+(S4)) products. This was revealed by the observed depletion of the yield of the O− anion and the appearance of a new O+ cation signal with a kinetic energy Etransl(O+) dependent on the time delay between the pump and probe lasers. This time-delay dependence of the dissociation dynamics on the ion-pair state has also been simulated, and the experimental and simulated results coincide very well over the experimental delay-time interval from about 130fsto20ps where two- or one-photon photodetachment takes place, corresponding to a change in the R(O+,O−) interatomic distance from 12 to about 900Å. This is one of the first implementations of a depletion scheme in femtosecond pump-probe experiments which could prove to be quite versatile and applicable to many femtosecond time-scale experiments.
The temperature fluctuations generated by viscous dissipation in an isotropic turbulent flow are studied using direct numerical simulation. It is shown that their scaling with Reynolds number is at odds with predictions from recent investigations. The origin of the discrepancy is traced back to the anomalous scaling of the dissipation rate fluctuations. Phenomenological arguments are presented which explain the observed results. The study shows that previously proposed models underpredict the variance of frictional temperature fluctuations by a factor proportional to the square of the Taylor-scale Reynolds number.
Cobalt and nitrogen co-doped carbon materials (Co@CN) have recently attracted significant attention as highly efficient noble-metal-free catalysts exhibiting a large application range. In a similar research interest, and taking into account the ever-increasing importance of bioethanol as a renewable raw material, here, we report the results on ethanol dehydrogenation to acetaldehyde over Co@NC catalysts. The catalyst samples were synthesized by a variety of affordable techniques, ensuring generation of various types of Co species incorporated in carbon, such as subnanosized cobalt sites and nano-sized particles of metallic cobalt and cobalt oxides. The catalytic activity was tested under both oxidative and non-oxidative gas-phase conditions at 200–450 °C using a fixed-bed flow reactor. The non-oxidative conditions proved to be much more preferable for the target reaction, competing, however, with ethanol dehydration to ethylene. Under specified reaction conditions, ethanol conversion achieved a level of 66% with 84% selectivity to acetaldehyde at 400 °C. The presence of molecular oxygen in the feed led mainly to deep oxidation of ethanol to COx, giving acetaldehyde in a comparatively low yield. The potential contribution of carbon itself and supported cobalt forms to the observed reaction pathways is discussed.
Pathogenesis of age-related macular degeneration (AMD), the leading cause of blindness in the world, remains poorly understood. This makes it necessary to create animal models for studying AMD pathogenesis and to design new therapeutic approaches. Here we showed that retinopathy in OXYS rats is similar to human AMD according to clinical signs, morphology, and vascular endothelium growth factor (VEGF) and pigment epithelium-derived factor (PEDF) genes expression. Clinical signs of retinopathy OXYS rats manifest by the age 3 months against the background of significantly reduced expression level of VEGF and PEDF genes due to the decline of the amount of retinal pigment epithelium (RPE) cells and alteration of choroidal microcirculation. The disruption in OXYS rats' retina starts at the age of 20 days and appears as reduce the area of RPE cells but does not affect their ultrastructure. Ultrastructural pathological alterations of RPE as well as develop forms of retinopathy are observed in OXYS rats from age 12 months and manifested as excessive accumulation of lipofuscin in RPE regions adjacent to the rod cells, whirling extentions of the basement membrane into the cytoplasm. These data suggest that primary cellular degenerative alterations in the RPE cells secondarily lead to choriocapillaris atrophy and results in complete loss of photoreceptor cells in the OXYS rats' retina by the age of 24 months.