Hengzongshu/ArticleAgent huggingface dataset:
Karyotype evolution in Carnivora is thoroughly studied by classical and molecular cytogenetics and supplemented by reconstructions of Ancestral Carnivora Karyotype (ACK). However chromosome painting information from two pinniped families (Odobenidae and Otariidae) is noticeably missing. We report on the construction of the comparative chromosome map for species from each of the three pinniped families: the walrus (Odobenus rosmarus, Odobenidae–monotypic family), near threatened Steller sea lion (Eumetopias jubatus, Otariidae) and the endemic Baikal seal (Pusa sibirica, Phocidae) using combination of human, domestic dog and stone marten whole-chromosome painting probes. The earliest karyological studies of Pinnipedia showed that pinnipeds were characterized by a pronounced karyological conservatism that is confirmed here with species from Phocidae, Otariidae and Odobenidae sharing same low number of conserved human autosomal segments (32). Chromosome painting in Pinnipedia and comparison with non-pinniped carnivore karyotypes provide strong support for refined structure of ACK with 2n = 38. Constructed comparative chromosome maps show that pinniped karyotype evolution was characterized by few tandem fusions, seemingly absent inversions and slow rate of genome rearrangements (less then one rearrangement per 10 million years). Integrative comparative analyses with published chromosome painting of Phoca vitulina revealed common cytogenetic signature for Phoca/Pusa branch and supports Phocidae and Otaroidea (Otariidae/Odobenidae) as sister groups. We revealed rearrangements specific for walrus karyotype and found the chromosomal signature linking together families Otariidae and Odobenidae. The Steller sea lion karyotype is the most conserved among three studied species and differs from the ACK by single fusion. The study underlined the strikingly slow karyotype evolution of the Pinnipedia in general and the Otariidae in particular.
Long-range chromatin interactions play an important role in transcription regulation.

Chromatin Interaction Analysis with Paired-End-Tag sequencing (ChIA-PET) is an emerging technology that has unique advantages in chromatin interaction analysis, and thus provides insight into the study of transcription regulation. This article introduces the experimental protocol and data analysis process of ChIA-PET, as well as discusses some applications using this technology.

It also unveils the direction of future studies based on this technology. Overall we show that ChIA-PET is the cornerstone to explore the three-dimensional (3D) chromatin structure, and certainly will lead the forthcoming wave of 3D genomics studies.
Composition of native gold and minerals in intergrowth of the Chudnoe Au-Pd-REE deposit (Subpolar Urals, Russia) was studied using optical microscopy, scanning electron microscopy, and electron microprobe analysis.

Five varieties of native gold have been identified, based on the set of impurity elements and their quantities, and on intergrown minerals. Native gold in rhyolites from the Ludnaya ore zone is homogeneous and contains only Ag (fineness 720‰, type I). It is in intergrowth with fuchsite or allanite and mertieite-II. In rhyolites from the Slavnaya ore zone, native gold is heterogeneous, has a higher fineness, different sets and contents of elements: Ag, Cu, 840–860‰ (type II); Ag, Cu, Pd, 830–890‰ (III); Ag, Pd, Cu, Hg, 840–870‰ (IV). It occurs in intergrowth with fuchsite, albite, and mertieite-II (type II), or albite, quartz, and atheneite (III), or quartz, albite, K-feldspar, and mertieite-II (IV). High-fineness gold (930–1000‰, type V) with low contents of Ag, Cu, and Pd or their absence occurs in the form as microveins, fringes and microinclusions in native gold II–IV. Tetra-auricupride (AuCu) is presented as isometric inclusions in native gold II and platelets in the decay structures in native gold III and IV. The preliminary data of a fluid inclusions study showed that gold mineralization at the Chudnoe deposit could have been formed by chloride fluids of low and medium salinity at temperatures from 105 to 230 °C and pressures from 5 to 115 MPa.

The formation of native gold I is probably related to fuchsitization and allanitization of rhyolites. The formation of native gold II-V is also associated with the same processes, but it is more complicated and occurred later with a significant role of Na-, Si-, and K-metasomatism. The presence of Pd and Cu in the ores and Cr in fuchsite indicates the important role of mafic-ultramafic magmatism.
The effect of palladium dispersion and nature of the support on catalytic performance in hydrogenation of nitrobenzene to aniline was studied. It was shown that the type of the support and modification of palladium with phosphorus make it possible to stabilize highly dispersed (1.5-2 nm) palladium particles in the metallic state, thus increasing the efficiency of new catalysts.
The aim of this study is to investigate the frequency of CYP2C19*2, *3 allelic variants, associated with poor response to clopidogrel, and CYP2C19*17, associated with excessive response to clopidogrel, in patients with acute coronary syndrome (ACS) from Siberia and Moscow regions of Russia.

The study included 512 ACS patients who were subsequently treated with coronary arterial stenting. The subjects assigned were from the cities of Central (Novosibirsk, Kemerovo), Eastern (Irkutsk), Northern (Surgut) Siberia regions and from Moscow region. The mean age of patients enrolled was 63.9±10.9 years. Among the assigned subjects, the proportion of men accounted for 80% and women 20%.

According to the results obtained in the present study, from 16% up to 27.5% of patients in different regions of Russia have at least one CYP2C19 'poor metabolizer' (PM) allele variant affecting clopidogrel metabolism and, therefore, suppressing its antiplatelet activity. CYP2C19*17 allele variant was identified with the frequency of 15.4% up to 33.3%. The study revealed the presence of statistically significant differences in CYP2C19*3 allele frequency between the Russian ethnic group patients from Eastern and Central Siberia (p=0.001; odds ratio=1.05 [95% confidence interval 1.01-1.09]). The study revealed statistically significant differences between the allele frequencies in Eastern and Central Siberia, which can probably be caused by a considerable number of Buryats inhabiting Eastern Siberia.
We are reporting about investigation of domestic 3 mm aperture periodically polled lithium niobate (PPLN) structures for cascaded mid-IR OPO.

Wide aperture periodically poled MgO-doped lithium niobate (LiNbO3) structures at multigrating, fan-out and multi fan-out configuration were prepared at "Labfer LTD". Four different PPLN structures were investigated and effective aperture for effective pumping was defined.

Laser source based on such structures can be used for special applications.
The problem of helical vortices description has the significant interest as from fundamental point of view as well for practice. In some sense this problem is close to the vortex ring one which attracted much more attention in last decades. The reviews on the vortex rings investigations can be found in recent papers [1,2] or in book by Akhmetov [3]. In particular, in series of papers by Kaplanskii with co-authors [4-6] there was considered the viscosity influence on the vortex ring evolution. Separate attention was paid to the low Reynolds number case and to high Reynolds number one, initial stage of viscous evolution and final one.

This paper presents first attempt for research on the diffusion and dynamics of a viscous helical vortex.
Objective – is to study the dynamics of blood pressure (BP) in the indigenous population of the Shors in response to the antihypertensive therapy with a β-blocker (metoprolol succinate), taking into account the polymorphism of the candidate genes of the sympathetic adrenal system (ADRB1 and ADRA2B). Design and methods. The study of the indigenous population (the Shors) living in the areas of Mountain Shoria (Kemerovo region) was carried out. Using continuous sampling method, 901 people (18 years old and above) were examined. Measurement of blood pressure was carried out in accordance with the recommendations of the Russian Society of Cardiology / the Russian Society of Hypertension (2010). The survey allowed identifying the number of respondents with arterial hypertension (AH) for further observation - 367 people (40.7%). The second screening was performed one year after the patients were included in the study. The criterion for putting the patient under prospective observation was regular intake of the medication prescribed (162 people). Antihypertensive therapy was prescribed by a cardiologist according to the recommendations of the Russian Society of Cardiology /the Russian Society of Hypertension (2010). It included taking a β1-selective medication, metoprolol succinate, from the group of β-adrenergic blockers in a dose of 100 mg per day. Gene polymorphism ADRB1 (c.145A> G, Ser49Gly, rs1801252) and ADRA2B (I/D, rs28365031) were tested using polymerase chain reaction. Results . The study of the indigenous population proves that there is an association between carrying the A allele in the homozygous state of the ADRB1 gene and achieving the target level of blood pressure (OR = 2.36) while taking a β-blocker (metoprolol succinate). In the Shor population ADRA2B gene polymorphism was not associated with the effective treatment of hypertension using this medication. Conclusion . An epidemiological study in Mountain Shoria has demonstrated an antihypertensive effect of metoprolol succinate in its dependence on the polymorphism of the candidate genes (ADRB1 and ADRA2B). They are coding the components of the sympathetic adrenal system which plays an important role in the pathogenesis of hypertension. Today there are no clinical algorithms for checking personalized sensibility to the β-blockers, and therefore further research in this area remains a topical subject.
The confluence of two scientific disciplines may lead to nomenclature conflicts that require new terms while respecting historical definitions. This is the situation with the current state of cytology and genomics, which offer examples of distinct nomenclature and vocabularies that require reconciliation. In this article, we propose the new terms C-scaffold (for chromosome-scale assemblies of sequenced DNA fragments, commonly named scaffolds) and scaffotype (the resulting collection of C-scaffolds that represent an organism's genome). This nomenclature avoids conflict with the historical definitions of the terms chromosome (a microscopic body made of DNA and protein) and karyotype (the collection of images of all chromosomes of an organism or species). As large-scale sequencing projects progress, adoption of this nomenclature will assist end users to properly classify genome assemblies, thus facilitating genomic analysis.
The evolution of the artificial wave packet in the supersonic boundary layer of swept wing was experimentally studied for the first time by hot-wire measurements. The localized disturbances were generated by pulse glow discharge. Analysis of the mean flow, integral dependences, and the averaged waveforms of wave packet in supersonic boundary layer of swept wing has been executed.
This work deals with the study of the gas phase effect on integral characteristics of a swirling two-phase flow with a precessing vortex core.

The formation conditions of a precessing vortex filament were modeled in hydrodynamic vortex chambers with a sudden expansion, where the flow was swirled by means of the vane and tangential swirlers. Experimental dependences of the frequency of vortex core precession and total pressure drop in the vortex chambers on gas content are obtained for different degrees of flow swirl.

The studies have shown that the main effect of air supply on the flow parameters is associated with separation of the lighter gas phase toward the vortex core center and formation of a gas core along the vortex axis. The effective separation of air from liquid into the gas core is particularly confirmed by the fact that the measured data are well generalized in dimensionless variables based on the liquid phase parameters.
Formation pressure of mixed hydrogen + methane, hydrogen + ethane and hydrogen + propane hydrates can be controlled by the gas phase composition allowing these hydrates to be considered as promising hydrogen storage containers.

At low methane (ethane) concentration in the gas phase, the hydrate of the cubic structure II is formed, and at the methane concentration exceeding 6 mol.% (1 mol.% for ethane) the cubic structure I is formed. The mass percentage of hydrogen in the hydrate phase depends on the second gas concentration in the gas phase as well as on the thermodynamic conditions of hydrate formation. At a low concentration of methane (ethane) in the gas phase, the mass percentage of hydrogen in the hydrate can reach 2.5 wt% at 250 K. The curves of the monovariant equilibrium 'gas-hydrate-ice Ih' for double hydrates are calculated and found to be in agreement with the available experimental data.

Thermodynamic properties of mentioned mixed hydrates allow to considering mixed hydrates as appropriate materials for hydrogen storage.
We introduce an extension to the Protege ontology editor, which allows for discovering concept definitions, which are not explicitly present in axioms, but are logically implied by an ontology. The plugin supports ontologies formulated in the Description Logic EL, which underpins the OWL 2 EL profile of the Web Ontology Language and despite its limited expressiveness captures most of the biomedical ontologies published on the Web.

The developed tool allows to verify whether a concept can be defined using a vocabulary of interest specified by a user. In particular, it allows to decide whether some vocabulary items can be omitted in a formulation of a complex concept.

The corresponding definitions are presented to the user and are provided with explanations generated by an ontology reasoner.
Flow cytometry method (FCM) is widely used for analysis of cell-derived microparticles (MPs). Numerous efforts are currently aimed to standardize these measurements among different instruments.

We push the FCM characterization of MPs to the limit based on rigorous simulation of measured signals. We measured forward- and side-scatter (FSC/SSC) signals and angle-resolved light-scattering profiles (LSPs) of polystyrene microspheres and MPs, including their aggregates, using a scanning flow cytometer (SFC). We used the Mie theory to (1) accurately evaluate instrument detection limits; (2) construct FSC/SSC gates for MPs in absolute scales of size and refractive index (RI); and (3) determine size and RI of individual spherical MPs. LSPs were used for advanced characterization, including differentiation of spherical and nonspherical particles.

The proposed absolute FSC/SSC gating is naturally standardized for any FCM instrument, given the knowledge of its optical system and leads to instrument-independent analysis of MPs. The inverse Mie problem has a unique solution only for some regions of size and RI and uncertainties rapidly increase with decreasing size and RI. The developed methods are applicable to any flow cytometer, but are limited by assumption of particle sphericity. The latter can be relaxed only if additional signals, such as LSP, are measured.
The Olkhon terrane in the Western Baikal area accommodates four types of carbonate-silicate mixtures: injection (protrusion), metamorphic-boudinated, mingling, and tectonite marble mélange. The outcrops of injection mélange consist of a carbonate matrix with inclusions of native silicic rocks found in the immediate vicinities, commonly cover large areas and lack any distinct linearity in the map view. Mélange of the metamorphic boudinage type comprises diopsidite and tremilote-diopsidite fragments in a dolomitic or calcite-dolomitic matrix. Its origin is apparently due to tectonism and related metamorphism of quartz sandstones in Neoproterozoic strata on the passive margin of the Siberian craton. Mingling mélange appears as calcite marble or carbonate-silicate (calciphyre) veins with metadolerite and granite inclusions of different sizes. The veins formed by intrusion of carbonate and silicate melt batches and subsequent fragmentation of silicate rocks that crystallized earlier. Marble tectonites localized in narrow zones record the late phase of ductile marble injection.
The Chatkal‐Kurama terrane represents a key region in understanding the tectonic evolution of the western Tian Shan.

In this contribution, we present new thermochronological data (zircon [U‐Th‐Sm]/He, apatite fission track, and apatite [U‐Th‐Sm]/He) and the associated thermal history models for 30 igneous samples from the Chatkal‐Kurama terrane within Uzbekistan and Tajikistan (west of the Talas‐Fergana Fault) and integrate our data with published data from the central Tian Shan (east of the Talas‐Fergana Fault).

The Chatkal‐Kurama terrane experienced a phase of rapid cooling during the Triassic‐Jurassic at ca. 225–190 Ma, which we interpret as a far‐field response to the closure of the Palaeo‐Asian Ocean or the accretion of the Qiangtang terrane on to the Eurasian margin. In the Late Jurassic to the Early Cretaceous, the Chatkal‐Kurama terrane experienced a period of tectonic stability and denudation, before transitioning into a period of marine incursions of the Paratethys Sea. In contrast, fast cooling is recorded for the Kyrgyz central Tian Shan to the east of the Talas‐Fergana Fault. The differing thermal histories at either side of the Talas‐Fergana Fault suggest that the fault induced a topographic divide during the Late Jurassic‐Early Cretaceous, with high relief in the east (Kyrgyz Tian Shan) and low relief to the west (Uzbek‐Tajik Tian Shan). Finally, the Chatkal‐Kurama terrane experienced renewed tectonic activity since ca. 30 Ma, related with the distant India‐Eurasia collision and Pamir indentation. The Cenozoic reactivation induced crustal tilting of the Chatkal‐Kurama terrane, progressively exposing deeper rocks to the southwest.
Aim. To study the influence of hypoglycemic therapy on hospital and long-term prognosis in patients with acute coronary syndrome (ACS) and diabetes type 2. Methods. The study included 63 patients with ACS and type 2 diabetes. All patients had a clinical examination, assessment of mortality risk and myocardial infarction on GRACE scale (Global Registry of Acute Coronary Events) and TIMI (Thrombolisis In Myocardial Infarction) in-hospital and six months after hospitalization. Results. Metformin is associated with a lower estimated risk of in-hospital mortality and within 6 months after discharge in patients with acute coronary syndrome on the background of type 2 diabetes and with less risk of adverse cardiovascular events within 14 days of their occurrence in patients with unstable angina pectoris on the background of diabetes. High daily doses of metformin have also been associated with a decrease in the estimated risk of in-hospital mortality and within 6 months after discharge in patients with ACS associated with diabetes. The inverse association between the daily dosage of metformin and the presence of angina pectoris in patients with ACS and diabetes type 2 indicates a protective effect of metformin high daily dosages in relation to the risk of complications within six months after the discharge from hospital. Conclusion. One of the important aspects of ACS treatment, along with effective therapy, is the impact on concomitant risk factors, including blood glucose control. The main groups of hypoglycemic drugs have currently been identified; their effect on cardiovascular events, long-term effects and long-term prognosis are being investigated.
The cross-section data are compared to theoretical predictions based on the state-of-the-art nucleon-nucleon potentials, combined with a three-nucleon force or the Coulomb interaction or carried out in a relativistic regime.

The differential cross section for the 1H(d,pp)n breakup reaction at deuteron beam energy of 340 MeV has been measured with the use of the WASA detector at COSY-Jülich. The set of proton-proton coincidences registered at the Forward Detector has been analyzed on a dense grid of kinematic variables, giving in total around 5600 data points.

The cross-section data are compared to theoretical predictions based on the state-of-the-art nucleon-nucleon potentials, combined with a three-nucleon force or the Coulomb interaction or carried out in a relativistic regime.
The new Zakharov–Resio–Pushkarev (ZRP) wind input source term Zakharov et al.(2012) is examined for its theoretical consistency via numerical simulation of the Hasselmann equation.

The results are compared to field experimental data, collected at different sites around the world, and theoretical predictions based on self-similarity analysis.

Consistent results are obtained for both limited fetch and duration limited statements.
A new phenomenological model of cyclic creep, which is suitable for applications involving finite creep deformations of the material, is proposed. The model accounts for the effect of the transient increase of the creep strain rate upon the load reversal. In order to extend the applicability range of the model, the creep process is fully coupled to the classical Kachanov-Rabotnov damage evolution. As a result, the proposed model describes all the three stages of creep. Large strain kinematics is described in a geometrically exact manner using the assumption of a nested multiplicative split, originally proposed by Lion for finite strain plasticity. The model is thermodynamically admissible, objective, and w-invariant. The implicit time integration of the proposed evolution equations is discussed. The corresponding numerical algorithm is implemented into the commercial FEM code MSC.Marc. The model is validated using this code; the validation is based on real experimental data on cyclic torsion of a thick-walled tubular specimen made of the D16T aluminium alloy. The numerically computed stress distribution exhibits a "skeletal point" within the specimen, which simplifies the analysis of test data.
We propose an experiment to search for a new gauge boson A' in e+e− annihilation by means of a positron beam incident on a gas hydrogen target internal to the bypass at the VEPP-3 storage ring. The search method is based on a missing mass spectrum in the reaction e+e−→ γA'. It allows observation of the A' signal independently of its decay modes and life time. The projected result of this experiment corresponds to an upper limit on the square of the coupling constant ε2=3⋅ 10−8 with a signal-to-noise ratio of two to one at an A' mass of 5–20 MeV .
Using the standard Balitsky-Fadin-Kuraev-Lipatov (BFKL) approach, with partial inclusion of next-to-leading order effects

we propose the inclusive hadroproduction of a Higgs boson and of a jet, featuring large transverse momenta and well separated in rapidity, as a new channel to probe the BFKL dynamics. Predictions are presented for cross-sections and azimuthal angle correlations in different kinematics configurations for the final-state transverse momenta

We find that the large energy scales provided by the emission of a Higgs boson stabilize the BFKL series