Hengzongshu/ArticleAgent huggingface dataset:
In all organisms, DNA glycosylases initiate base excision repair pathways resulting in removal of aberrant bases from DNA. Human SMUG1 belongs to the superfamily of uracil-DNA glycosylases catalyzing the hydrolysis of the N-glycosidic bond of uridine and uridine lesions bearing oxidized groups at C5: 5-hydroxymethyluridine (5hmU), 5-formyluridine (5fU), and 5-hydroxyuridine (5hoU). An apurinic/apyrimidinic (AP) site formed as the product of an N-glycosylase reaction is tightly bound to hSMUG1, thus inhibiting the downstream action of AP-endonuclease APE1. The steady-state kinetic parameters (kcat and KM; obtained from the literature) correspond to the enzyme turnover process limited by the release of hSMUG1 from the complex with the AP-site.

In the present study, our objective was to carry out a stopped-flow fluorescence analysis of the interaction of hSMUG1 with a DNA substrate containing a dU:dG base pair to follow the pre-steady-state kinetics of conformational changes in both molecules. A comparison of kinetic data obtained by means of Trp and 2-aminopurine fluorescence and Förster resonance energy transfer (FRET) detection allowed us to elucidate the stages of specific and nonspecific DNA binding, to propose the mechanism of damaged base recognition by hSMUG1, and to determine the true rate of the catalytic step.

Our results shed light on the kinetic mechanism underlying the initiation of base excision repair by hSMUG1 using the 'wedge' strategy for DNA lesion search.
The cation radicals formed when methionine and N-acetylmethionine quench the photo-excited triplet state of 4-carboxybenzophenone in aqueous solution are characterized by their g-factors and hyperfine coupling constants.It is shown that the radicals exist as either cyclic or linear structures depending on the amino acid and the pH of the medium.The study is based on the analysis of the dynamic nuclear polarization generated in the spin-selective decay of the transient radicals and its dependence on the external magnetic field.The requirements and scope of this method are discussed.
The article presents data on the fauna and ecology of earthworms in the Kuznetsk-Salair mountain area. The paper describes the current condition and activities of the conservation of the two rare endemic species listed in the Red Data Book of the Russian Federation – Eisenia salairica and Eisenia malevici. The characteristics of their habitats are given. Data on molecular genetic analysis of Eisenia salairica are presented.
General features of spontaneous baryogenesis are studied. The relation between the time derivative of the (pseudo) Goldstone field and the baryonic chemical potential is revisited.

It is shown that this relation essentially depends upon the representation chosen for the fermionic fields with nonzero baryonic number (quarks). The calculations of the cosmological baryon asymmetry are based on the kinetic equation generalized to the case of nonstationary background. The effects of the finite interval of the integration over time are also taken into consideration.

All these effects combined lead to a noticeable deviation of the magnitude of the baryon asymmetry from the canonical results.
A comparison of the structure and sensitivity of humidity sensors prepared from graphene (G)-PEDOT: PSS (poly (3,4-ethylenedioxythiophene)) composite material on flexible and solid substrates is performed. Upon an increase in humidity, the G: PEDOT: PSS composite films ensure a response (a linear increase in resistance versus humidity) up to 220% without restrictions typical of sensors fabricated from PEDOT: PSS. It was found that the response of the examined sensors depends not only on the composition of the layer and on its thickness but, also, on the substrate used. The capability of flexible substrates to absorb the liquid component of the ink used to print the sensors markedly alters the structure of the film, making it more porous; as a result, the response to moisture increases. However, in the case of using paper, a hysteresis of resistance occurs during an increase or decrease of humidity; that hysteresis is associated with the capability of such substrates to absorb moisture and transfer it to the sensing layer of the sensor. A study of the properties of G: PEDOT: PSS films and test device structures under deformation showed that when the G: PEDOT: PSS films or structures are bent to a bending radius of 3 mm (1.5% strain), the properties of those films and structures remain unchanged. This result makes the composite humidity sensors based on G: PEDOT: PSS films promising devices for use in flexible and printed electronics.
Unusual plasmons, excited by an evanescent wave in narrow slits in a periodic array of nanowires overlying a dielectric substrate, are found from Maxwell's equations by a modified boundary element method. The evanescent wave appears when the incident plane wave runs onto the boundary from the substrate under the angle of total internal reflection. On the basis of Floquet's theorem, the effective Green's function is derived by reducing the infinite structure to one elementary cell. The method is tested by simulation of the known Rayleigh–Wood's anomalies. The plasmon resonance is found to have a sharp dependence on the angle of incidence.
We demonstrate and numerically characterize a set of single-layer metasurfaces based on capacitively-coupled bent metal strips deposited on a thin polypropylene film operating as band-stop filters at 139.1 GHz. Due to a high inter-digital coupling capacitance between neighboring inclusions formed by the strips, the resonance of the metasurface becomes highly sensitive to the incidence angle of a plane wave. At the same time, a narrow-band resonance is achieved by reducing the conductor length along the vertical polarization direction of the incident wave. We propose to use both properties for designing a sensor, which analyses the thickness of a thin slab of a known substance deposited over the structure by tracking the angle at which the minimum of transmission can be measured at a single frequency. We compare three versions of the proposed geometry by numerical calculation of the transmission coefficient depending on both the incidence angle and analyte slab’s thickness. We select the most suitable structure for sensing.
The first list of the rare grasshoppers of the Baraba and Kulunda steppes is presented. Two sets of distribution data are compared: (1) for the first half of 20 th century and (2) for 1972–2019.

A series of digital maps was generated by MapInfo 12.03.

The distribution patterns of several species, namely Asiotmethis muricatus (Pallas), Notostaurus albicornis (Eversmann), Eremippus simplex (Eversmann), Myrmeleotettix antennatus (Fieber), Gomphocerippus rufus (Linnaeus), Mesasippus arenosus (Bey-Bienko), Mecostethus parapleurus (Hagenbach), Locusta migratoria Linnaeus, did not change significantly. Four taxa ( Asiotmethis jubatus (Uvarov), Arcyptera fusca (Pallas), Stenobothrus carbonarius (Eversmann), Sphingonotus coerulipes Uvarov) were relatively often in the first half of 20 th century and nowadays they are extremely rare. Two species, namely Megaulacobothrus aethalinus (Zubovsky) and Aeropedellus variegatus (Fischer de Waldheim), were recently found near the south-eastern and north-eastern boundaries of the region respectively. There are also the type localities of Asiotmethis jubatus and Mesasippus arenosus in the Kulunda steppe.
Single crystals of furan/phenylene co-oligomers are among the most promising highly-emissive materials for applications in various optoelectronic devices. In this work, we synthesized and studied furan/phenylene co-oligomers with the same conjugated core 1,4-bis(5-phenylfuran-2-yl)benzene and methyl substituents at p- and m-positions of the terminal phenyls. The effect of substituents on the crystal packing, charge transport and luminescence of the single crystals was studied. Compared to the unsubstituted compound, the methyl-substituted co-oligomers demonstrated improved thermostability and enhanced photoluminescence, which we assign to J-aggregation resulting from the strong inclination of the molecules against the main crystal facet. The charge mobility in single crystal organic field-effect transistors decreased upon the inclination of the molecules. We conclude that the molecular tilt angle, intermolecular distances and interactions in crystals of heteroaryl-containing linear conjugated oligomers can be controlled by the introduction of end methyl groups in the appropriate positions.
The cover picture shows spectroscopic methods to characterize mobile protons in a proton-conductive porous metal–organic frameworks with a regular crystalline structure.

As we use optical and radio-frequency instruments to probe the night sky secrets, we combine different types of molecular spectroscopies to shed light on the mysteries of the proton transfer in solids.

The long range ordering of host materials facilitates to uncover the molecular mechanism of the charged proton species formation and migration.
A study of the charge conjugation and parity ($CP$) properties of the interaction between the Higgs boson and top quarks is presented. Higgs bosons are identified via the diphoton decay channel ($H\ensuremath{\rightarrow}\ensuremath{\gamma}\ensuremath{\gamma}$), and their production in association with a top quark pair ($t\overline{t}H$) or single top quark ($tH$) is studied.

The analysis uses $139\text{ }\text{ }{\mathrm{fb}}^{\ensuremath{-}1}$ of proton--proton collision data recorded at a center-of-mass energy of $\sqrt{s}=13\text{ }\text{ }\mathrm{TeV}$ with the ATLAS detector at the Large Hadron Collider.

Assuming a $CP$-even coupling, the $t\overline{t}H$ process is observed with a significance of 5.2 standard deviations. The measured cross section times $H\ensuremath{\rightarrow}\ensuremath{\gamma}\ensuremath{\gamma}$ branching ratio is ${1.64}_{\ensuremath{-}0.36}^{+0.38}(\text{stat}{)}_{\ensuremath{-}0.14}^{+0.17}(\text{sys})\text{ }\text{ }\mathrm{fb}$, and the measured rate for $t\overline{t}H$ is ${1.43}_{\ensuremath{-}0.31}^{+0.33}(\text{stat}{)}_{\ensuremath{-}0.15}^{+0.21}(\text{sys})$ times the Standard Model expectation. The $tH$ production process is not observed and an upper limit on its rate of 12 times the Standard Model expectation is set. A $CP$-mixing angle greater (less) than 43 $(\ensuremath{-}43)\ifmmode^\circ\else\textdegree\fi{}$ is excluded at 95% confidence level.
In this article, we briefly summarize the experiments performed during the first Run of the Advanced Wakefield Experiment, AWAKE, at CERN (European Organization for Nuclear Research). The final goal of AWAKE Run 1 (2013 - 2018) was to demonstrate that \unit[10-20]{MeV} electrons can be accelerated to GeV-energies in a plasma wakefield driven by a highly-relativistic self-modulated proton bunch.

We describe the experiment, outline the measurement concept

and present first results. Last, we outline our plans for the future.
We propose an extrapolation technique that allows accuracy improvement of discrete dipole approximation computations. The performance of this technique was studied empirically on the basis of extensive simulations for five test cases using many different discretizations. The quality of the extrapolation improves with refining discretization, reaching extraordinary performance especially for cubically shaped particles. A 2-order-of-magnitude decrease of error is demonstrated. We also propose estimates of the extrapolation error, which are proven to be reliable. Finally, we propose a simple method to directly separate shape and discretization errors and illustrate this for one test case.
Stress negatively affects processes of synaptic plasticity and is a major risk factor of various psychopathologies such as depression and anxiety. HOMER1 is an important component of the postsynaptic density: constitutively expressed long isoforms HOMER1b and HOMER1c bind to group I metabotropic glutamate receptors MGLUR1 (GRM1) and MGLUR5 and to other effector proteins, thereby forming a postsynaptic protein scaffold.

Activation of the GLUR1–HOMER1b,c and/or GLUR5–HOMER1b,c complex regulates activity of the NMDA and AMPA receptors and Ca 2+ homeostasis, thus modulating various types of synaptic plasticity. Dominant negative transcript Homer1a is formed as a result of activity-induced alternative termination of transcription. Expression of this truncated isoform in response to neuronal activation impairs interactions of HOMER1b,c with adaptor proteins, triggers ligand-independent signal transduction through MGLUR1 and/or MGLUR5, leads to suppression of the AMPA- and NMDA-mediated signal transmission, and thereby launches remodeling of the postsynaptic protein scaffold and inhibits long-term potentiation.

The studies on animal models confirm that the HOMER1a-dependent remodeling most likely plays an important part in the stress susceptibility, whereas HOMER1a itself can be regarded as a neuroprotector. In this review article, we consider the effects of different stressors in various animal models on HOMER1 expression as well as impact of different HOMER1 variants on human behavior as well as structural and functional characteristics of the brain.
Revisiting the Incorporation of Ti(IV) in UiO-type Metal–Organic Frameworks: Metal Exchange versus Grafting and Their Implications on Photocatalysis

Metal Exchange versus Grafting approaches for Ti(IV) incorporation in UiO-type MOFs

Implications on Photocatalysis performance of the different incorporation methods
Model bimetallic Pd-Au/HOPG catalysts have been investigated in the CO oxidation reaction using a combination of NAP XPS and MS techniques.

The redistribution of Au and Pd on the surface depending on the reaction conditions has been demonstrated using NAP XPS. Heating the sample under reaction conditions above 150 °C decomposes the Pd-CO state due to CO desorption and reaction and simultaneous Pd-Au alloy formation on the surface takes place. Cooling back down to RT results in reversible Pd segregation due to Pd-CO formation.

The samples have shown catalytic activity at temperatures above 150 °C. The Pd enrichment of the bimetallic particles' surface under reaction gas mixture has been shown. Apparently, CO adsorption induces Pd segregation on the surface. It has been shown that in situ studies are necessary for investigation of the active sites in Pd-Au bimetallic systems.
The basic conservation laws in the Green–Nagdi model of shallow-water theory are derived from the two-dimensional integral conservation laws of mass and the total momentum describing the plane-parallel flow in an ideal incompressible fluid above a horizontal bottom.

This conclusion is based on the concept of a local hydrostatic approximation, which generalizes the concept of the long-wavelength approximation

and is used for analyzing the applicability of the Green–Nagdi equations in modeling the wave flows with undular bores.
The objective of the study: to study rs2227983 polymorphism of EGFR gene in patients with allergic asthma and healthy individuals.

Subjects and Methods. 179 patients suffering from allergic asthma were included in the study. The diagnosis and degree of severity were established in accordance with the GINA recommendations. The Control Group included apparently healthy individuals (n = 217). Patients with allergic asthma underwent standard laboratory and instrumental examinations and DNA typing.

Results. A statistically significant predominance of AG genotype frequency in the group of patients with allergic asthma, including women, versus the group of healthy individuals, was established. AG rs2227983 genotype of EGFR gene was found to be significantly more common in patients with mild and moderate allergic asthma including women, than in healthy individuals, including women. Conclusion. The association of rs2227983 polymorphism of EGFR gene with allergic asthma has been established. A homozygous GG genotype may play a protective role against the disease.
We study charmless B+ meson decays to the pΛ¯π+π− final state using a 605 fb−1 data sample collected at the Υ(4S) resonance with the Belle detector at the KEKB asymmetric-energy e+e− collider.

There are significant signals found with the pΛ¯ mass peaking near threshold.

The observed branching fraction for nonresonant B+→pΛ¯π+π− is (5.92−0.84+0.88(stat)±0.69(syst))×10−6 with a significance of 9.1 standard deviations. We also observe the intermediate three-body decay B+→pΛ¯ρ0 with a branching fraction of (4.78−0.64+0.67(stat)±0.60(syst))×10−6 and a significance of 9.5 standard deviations, and find a hint of a B+→pΛ¯f2(1270) signal. No other intermediate three-body decay is found in this study.
A method is developed for calculating the rate of homogeneous nucleation in a supersaturated vapor in which the molecular dynamics method and the nucleation theorem are combined. This approach allows us to predict the experimental data with good accuracy.
A measurement of the number of J/ψ events collected with the BESIII detector in 2009 and 2012 is performed using inclusive decays of the J/ψ. The number of J/ψ events taken in 2009 is recalculated to be (223.7 ± 1.4) × 106, which is in good agreement with the previous measurement, but with significantly improved precision due to improvements in the BESIII software. The number of J/ψ events taken in 2012 is determined to be (1086.9 ± 6.0) × 106. In total, the number of J/ψ events collected with the BESIII detector is measured to be (1310.6 ± 7.0) × 106, where the uncertainty is dominated by systematic effects and the statistical uncertainty is negligible.
CaGd 2 (MoO 4 ) 4 :Er 3+ /Yb 3+ phosphors with the doping concentrations of Er 3+ and Yb 3+ ( x = Er 3+ + Yb 3+ , Er 3+ = 0.05, 0.1, 0.2, and Yb 3+ = 0.2, 0.45) have been successfully synthesized by the microwave sol–gel method, and the crystal structure refinement and upconversion photoluminescence properties have been investigated. The synthesized particles, being formed after heat‐treatment at 900°C for 16 h, showed a well‐crystallized morphology. Under the excitation at 980 nm, CaGd 2 (MoO 4 ) 4 :Er 3+ /Yb 3+ particles exhibited strong 525 and 550‐nm emission bands in the green region and a weak 655‐nm emission band in the red region. The Raman spectrum of undoped CaGd 2 (MoO 4 ) 4 revealed about 15 narrow lines. The strongest band observed at 903 cm −1 was assigned to the ν 1 symmetric stretching vibration of MoO 4 tetrahedrons. The spectra of the samples doped with Er and Yb obtained under 514.5 nm excitation were dominated by Er 3+ luminescence preventing the recording Raman spectra of these samples. Concentration quenching of the erbium luminescence at 2 H 11/2 → 4 I 15/2 and 4 S 3/2 → 4 I 15/2 transitions in the CaGd 2 (MoO 4 ) 4 :Er 3+ /Yb 3+ crystal structure was established to be approximately at the 10 at.% doping level.
Blastic plasmacytoid dendritic cell neoplasm (BPDCN) is a malignancy with high frequency of skin involvement.

Based on clinical presentation, laboratory investigations, and histological examination of skin and bone marrow biopsy, a diagnosis of BPDCN with the involvement of skin, bone marrow, and central nervous system was made. The patient was put on acute lymphoblastic leukemia-like chemotherapy and achieved complete remission in November 2016, the eruption regressed. In January 2017, allogeneic bone marrow transplantation from matched sibling was performed.

This clinical case demonstrates the importance of laboratory tests. Histological examination helps to clarify a diagnosis of cutaneous lymphoma; however, a specific type of lymphoma needs immunohistochemical analysis. In our case, BPDCN at the initial stage presented like a systemic vasculitis.
Background .Heat shock effects can initiate apoptosis of oocytes and corpus luteum cells in mammalian ovaries. During folliculogenesis, follicular apoptosis is regulated by Bcl-2 and BAX proteins which are key effectors of granular cell death. Mechanisms of disruption of the ovarian corpus luteum development under heat stress remain largely unclear. Aim of the research : to identify the expression features of anti-apoptotic Bad and proapoptotic Bcl-2 proteins in the rat ovarian luteocytes in the acute (by day 3) and recovery (by days 7 and 14) periods after a single exposure of experimental hyperthermia (EH) (rectal temperature 43.5 °C). Materials and methods . The expression of Bad and Bcl-2 was determined immunohistochemically using an indirect two-stage streptavidin-biotin method. Results . On day 3 after EH, the expression areas of both Bad and Bcl-2 increased 2-fold, but the ratio of Bcl-2/Bad areas did not change, indicating that the intensity of apoptosis along the mitochondrial pathway in luteocytes in the acute period was maintained within physiological values. On day 7, the Bad and Bcl-2 expression areas remained at the level of day 3, but the Bcl-2/Bad index decreased, indicating the activation of the apoptosis internal pathway in the ovarian corpus luteum cells. By day 14, the protein expression areas decreased (Bad – by 1.7 times, Bcl-2 – by 3.2 times) compared to the acute period, and the Bcl-2/Bad index decreased by 2 times compared to the control and the acute period group. Conclusion . The observed predominance of proapoptotic Bad protein over antiapoptotic Bcl-2 in luteocytes on day 14 after EH indicates the anti-apoptotic protection violation, which leads to the apoptosis mitochondrial pathway activation of the latter. A decrease in Bcl-2 expression can be regarded as a manifestation of the defective luteocytes removal mechanism and the body’s desire to normalize the ovarian-uterine cycle disrupted by high temperature exposure.
Chromosomal localization of ribosomal RNA coding genes has been studied by using FISH (fluorescence in situ hybridization) in 21 species from the genus Chironomus Meigen, 1803. Analysis of the data has shown intra- and interspecific variation in number and location of 5.8S rDNA hybridization sites in 17 species from the subgenus Chironomus and 4 species from the subgenus Camptochironomus Kieffer, 1914. In the majority of studied species the location of rDNA sites coincided with the sites where active NORs (nucleolus organizer regions) were found. The number of hybridization sites in karyotypes of studied chironomids varied from 1 to 6. More than half of the species possessed only one NOR (12 out of 21). Two rDNA hybridization sites were found in karyotypes of five species, three - in two species, and five and six sites - in one species each. NORs were found in all chromosomal arms of species from the subgenus Chironomus with one of them always located on arm G. On the other hand, no hybridization sites were found on arm G in four studied species from the subgenus Camptochironomus. Two species from the subgenus Chironomus - Chironomusbalatonicus Devai, Wuelker & Scholl, 1983 and Chironomus "annularius" sensu Strenzke, 1959 - showed intraspecific variability in the number of hybridization signals. Possible mechanisms of origin of variability in number and location of rRNA genes in the karyotypes of species from the genus Chironomus are discussed.