Hengzongshu/ArticleAgent huggingface dataset:
Approach for in vivo real-time assessment of tumor tissue extracellular pH (pH(e)), redox, and intracellular glutathione based on L-band EPR spectroscopy using dual function pH and redox nitroxide probe and disulfide nitroxide biradical, is described. These parameters were monitored in PyMT mice bearing breast cancer tumors during treatment with granulocyte macrophage colony-stimulating factor. It was observed that tumor pH(e) is about 0.4 pH units lower than that in normal mammary gland tissue. Treatment with granulocyte macrophage colony-stimulating factor decreased the value of pH(e) by 0.3 units compared with PBS control treatment. Tumor tissue reducing capacity and intracellular glutathione were elevated compared with normal mammary gland tissue. Granulocyte macrophage colony-stimulating factor treatment resulted in a decrease of the tumor tissue reducing capacity and intracellular glutathione content. In addition to spectroscopic studies, pH(e) mapping was performed using recently proposed variable frequency proton-electron double-resonance imaging. The pH mapping superimposed with MRI image supports probe localization in mammary gland/tumor tissue, shows high heterogeneity of tumor tissue pH(e) and a difference of about 0.4 pH units between average pH(e) values in tumor and normal mammary gland. In summary, the developed multifunctional approach allows for in vivo, noninvasive pH(e), extracellular redox, and intracellular glutathione content monitoring during investigation of various therapeutic strategies for solid tumors.
A vacuum-insulated tandem accelerator was used to observe in situ blistering during 2-MeV proton irradiation of metallic samples to a fluence of up to 6.7 × 1020 cm−2. Samples consisting of copper of different purity, tantalum and tantalum-copper compounds were placed on the proton beam path and forced to cool. The surface state of the samples was observed using a charge-coupled device camera with a remote microscope. Thermistors, a pyrometer and an infrared camera were applied to measure the temperature of the samples during irradiation. After irradiation, the samples were analyzed on an X-ray diffractometer, laser and electron microscopes. The present study describes the experiment, presents the results obtained and notes their relevance and significance in the development of a lithium target for an accelerator-based neutron source, for use in boron neutron capture therapy of cancer.
The use of plasma emission cathode in the conjunction with a multiple apertured electron optical system (EOS) is promising for the multi-MW class electron beams of a large cross-sectional area. In a multi-aperture source, the beam parameters could be raised simply due to increase of the number of apertures (i.e. an effective emission area), if a uniformity of the electron emission over a large-area plasma cathode is ensured.

In the presented paper, the cross-sectional distribution of the emission current density was investigated using the X-ray diagnostic technique for two versions of the diode-type EOS, with electrodes performed as flat molybdenum 'grids'. The first one had 241 apertures arranged hexagonally inside a circle with a diameter of 8.3 cm and the second had 499 apertures within a circle of 11.8cm diameter. The emission plasma is produced using a single arc-discharge plasma generator placed on the axis at 20 cm from the EOS.

It was demonstrated that multi-aperture systems with a single on-axis plasma generator can be effectively employed to obtain large-area beams, even in the presence of the guiding magnetic field. All apertures are emitting in the 499-apertured EOS. The beam current density is quite uniform up to the radius 2.5cm and gradually decreases to the periphery.
Inner Tracker of Novosibirsk Super Charm-Tau Factory Detector has to measure momenta of soft hadrons, which do not reach the drift chamber; complement the drift chamber in measuring the momenta; detect secondary vertices of short-lived particles. Thus, proper choice of the option for the Inner Tracker is of significant interest. The simulation of charged pions propagation in the perpendicular direction to the beam axis was carried out with DD4HEP program based on GEANT4. Three options were considered: 4-layered Silicon microstrip detector, 4-layered cylindrical Gas Electron Multiplier (GEM) detector and Time Projection Chamber (TPC). The simulated Detector was located in 1.5 T magnetic field directed along beam axis. The simulation shows that pions with initial momenta less than 50 MeV /c do not pass through the beampipe and cannot be registered. Pions with momenta above 65 MeV /c leave energy depositions in all 4 layers of the Inner Tracker based on Silicon, and their trajectories can be reconstructed. Cylindrical GEM detector provides reconstruction possibility (hits in 4 layers) for pions with momenta more than 60 MeV /c. TPC with thin inner wall provides reconstruction of pions with momenta higher than 55 MeV /c. However, in this case the reconstruction procedure will be much more complicated due to a large number of background particle tracks in the sensitive volume.
The paper considers the paleohydrogeochemistry of the Upper Jurassic sediments of the Arctic regions of Western Siberia. On the territory under investigation during the late Jurassic marine sedimentation was predominate, the sea depths reached 400 m in the Volga time. Under these conditions, syngenetic sodium thalassogenic chloride waters were buried, in some places with a high content of magnesium, the salinity of which reached 35-40 g/dm3. A comparative analysis of the buried syngenetic waters of the Oxford basin and data on the modern hydrogeochemistry of groundwater of the Upper Jurassic aquiferous complex revealed very significant differences. The analysis of distribution value the total mineralization allowed to establish areas of positive and negative anomalies. In the first case, most of the anomalies are associated with the processes of vertical discharge of groundwater of the Lower Middle Jurassic and Paleozoic aquifers in the zones of tectonic disturbances. Negative anomalies regularly trace the clay boundary of the Oxford regional reservoir, expanding significantly in the north-east direction. Their nature is associated with the development of processes of elision water exchange and thermal dehydration of clay minerals.
Generation of induced pluripotent stem (iPS) cells expanded possibilities of pluripotency and early development studies. Generation of order Carnivora iPS cells from dog ( Canis lupus familiaris ), snow leopard ( Panthera uncia ), and American mink ( Neovison vison ) was previously reported. Pinnipeds are representatives of the suborder Caniformia sharing conservative genomes. There are several ways to deliver reprogramming transcription factors: RNA, proteins, plasmids, viral vectors etc. The most eﬀective delivery systems for mouse and human cells are based on viral vectors.

The aim of the current study was to examine conditions of pinniped fbroblast reprogramming. We compared a lentiviral vector which integrates into the genome and a Sendai virus­based vector, CytoTune EmGFP Sendai Fluorescence Reporter. The main advantage of Sendai virus­based vectors is that they do not integrate into the genome. We performed delivery of genetic constructions carrying ﬂuorescent proteins to fbroblasts of seven Pinnipeds: northern fur seal ( Callorhinus ursinus ), Steller sea lion ( Eumetopias jubatus ), walrus ( Odobenus rosmarus ), bearded seal ( Erignathus barbatus ), Baikal seal ( Pusa sibirica ), ringed seal ( Phoca hispida ), and spotted seal ( Phoca largha ). We also transduced American mink ( N. vison ), human ( Homo sapiens ), and mouse ( Mus musculus ) fbroblasts as a control.

We showed that the Sendai virus­based transduction system provides transgene expression one­two orders of magnitude higher than the lentiviral system at a comparable multiplicity of infection. Also, transgene expression after Sendai virus­based transduction is quite stable and changes only slightly at day four compared to day two. These data allow us to suggest that Sendai virus­based vectors are preferable for generation of Pinniped iPS cells.
A search for new charged massive gauge bosons, W′, is performed with the ATLAS detector at the LHC. Data were collected in proton–proton collisions at a center-of-mass energy of s=13 TeV and correspond to an integrated luminosity of 36.1 fb−1.

This analysis searches for W′ bosons in the W′→tb¯ decay channel in final states with an electron or muon plus jets. The search covers resonance masses between 0.5 and 5.0 TeV and considers right-handed W′ bosons.

No significant deviation from the Standard Model (SM) expectation is observed and upper limits are set on the W′→tb¯ cross section times branching ratio and the W′ boson effective couplings as a function of the W′ boson mass. For right-handed W′ bosons with coupling to the SM particles equal to the SM weak coupling constant, masses below 3.15 TeV are excluded at the 95% confidence level. This search is also combined with a previously published ATLAS result for W′→tb¯ in the fully hadronic final state. Using the combined searches, right-handed W′ bosons with masses below 3.25 TeV are excluded at the 95% confidence level.
It is known that the circadian rhythm of melatonin production depends on the intensity of illumination. Violation of the light regime leads to suppression of melatonin synthesis and the development of desynchronosis, which increases the risk of developing a number of pathologies. In this regard, it is relevant to search for opportunities to restore disturbed circadian rhythms and, especially, to correct immune dysfunctions that occur in these situations.

The aim of this study was to examine the effect of a complex of melatonin, aluminum oxide and polymethylsiloxane on the lymphocytes of the spleen of mice kept under round-the-clock lighting. Materials and methods . Mice of the C57Bl/6J line were kept under round-the-clock lighting for 14 days, against which they were intragastrically injected with distilled water, aluminum oxide with polydimethylsiloxane, melatonin and a complex of melatonin, aluminum oxide and polymethylsiloxane (a new drug developed by the Research Institute of Clinical and Experimental Lymphology – Branch of the Federal Research Center Institute of Cytology and Genetics SB RAS; Patent of Russian Federation No. 2577580, 2016), represented by a complex of porous material (aluminum oxide with polydimethylsiloxane) and melatonin, immobilized in the pores, from which it is gradually released in a liquid medium. Intact animals kept under the light regime of ST 12/12 and under round-the-clock lighting served as a control. Immunophenotyping of spleen B- and T-lymphocytes was performed on a flow cytofluorimeter with monoclonal antibodies APC CD3 and FITC CD19. For studying the distribution of cells by stages of the cell cycle in splenocytes, the amount of intracellular DNA was measured by the level of inclusion of propidium iodide.

The complex of melatonin, aluminum oxide and polymethylsiloxane has additional immunotropic properties in relation to the modifier molecule, which, apparently, are due to the joint immunostimulating effect of melatonin and the lymphostimulating effect of the sorbent. Melatonin in the composition of the complex shows its properties more stably.
Experimental simulation of the inhomogeneity of the incident flow localized in time and space on a bluff tear-drop airfoil is carried out.

It is shown that in the region outside the aerodynamic wake of the tear-drop airfoil, the presence of inhomogeneity leads to an increase in the amplitudes of velocity fluctuations in a wide frequency range. In the region inside the wake, the presence of inhomogeneity leads to the suppression of velocity fluctuations at the frequency of vortex shedding by a factor of 2–3 in amplitude.

The intensification of fluctuations at frequencies above the main one was also noted, and there was no effect on fluctuations at lower frequencies.
Expectations of unbounded functions of dependent nonnegative integer-valued random variables are approximated by the expectations of the functions of independent copies of these random variables. The Lindeberg method is used.
classical model of ideal and viscous gas and dispersed phase of spherical particles

On the basis of linear approximation of parameters of mixture additional relations describing interphase interactions are obtained

interphase interactions are obtained
Variability of heading date may assist in wheat adaptation to local environments. Thereafter, discovery of new heading date determinants is important for cereal improvement. In this study we used common wheat cultivar Chinese Spring (CS) and the substitution line of CS with 5B chromosome from T. dicoccoides (CS-5Bdic), different in their heading date by two weeks, to detect determinants of heading date on 5B chromosome. The possible influence of the VRN-B1 gene, the most powerful regulator of flowering, located on 5B chromosome, to differences in heading time between CS and CS-5Bdic was studied. The sequencing of this gene from CS-5Bdic showed that an insertion of a nucleotide triplet produced an additional amino acid in the corresponding protein. No changes in the transcription levels of each homoeologous VRN-1 loci were found in CS-5Bdic by comparison with CS. To ascertain the loci determining heading date difference, a set of 116 recombinant inbred 5В chromosomal lines as a result of hybridization of CS with CS-5Bdic were developed and their heading dates were estimated. Using the Illumina Infinium 15 k Wheat platform, 379 5B-specific polymorphic markers were detected and a genetic map with 82 skeletal markers was constructed. Phenotype (heading date) – genotype association analysis revealed seventy eight markers in pericentromeric region of 5B chromosome significantly associated with heading date variation. Based on this estimation and synteny with model crop genomes we identified the three best candidate genes: WRKY, ERF/AP2 and FHY3/FAR1. We supposed that the difference in activity of WRKY, ERF/AP2 and/or FHY3/FAR1 transcription factors between CS and CS-5Bdic to be a probable reason for the observed difference in heading dates. Data obtained in this study provide a good basis for the subsequent investigation of heading time pathways in wheat.
Drosophila melanogaster polytene chromosomes display specific banding pattern; the underlying genetic organization of this pattern has remained elusive for many years.

In the present paper, we analyze 32 cytology-mapped polytene chromosome interbands. We estimated molecular locations of these interbands, described their molecular and genetic organization. We developed a special algorithm to computationally process protein localization data generated by the modENCODE project.

Polytene chromosome interbands contain the 5' ends of housekeeping genes. Interbands display preferential 'head-to-head' orientation of genes. They are enriched for 'broad' class promoters characteristic of housekeeping genes and associate with open chromatin proteins and Origin Recognition Complex (ORC) components. Comparison of expression patterns shows that genes mapping to late-replicating dense bands are generally tissue-specific, whereas genes whose promoter regions map to interbands are represented by ubiquitously active genes. Analysis indicates that transcripts from interband-mapping genes are present in most tissues and cell lines. Drosophila genome has about 5700 sites that demonstrate all the features shared by the interbands cytologically mapped to date.
We report on a search for the charmless decays B+→ϕπ+ and B0→ϕπ0 that are strongly suppressed in the standard model.

The analysis is based on a data sample of 657×106 B¯¯¯B pairs collected at the Υ(4S) resonance with the Belle detector at the KEKB asymmetric-energy e+e− collider.

We find no significant signal and set upper limits of 3.3×10−7 for B+→ϕπ+ and 1.5×10−7 for B0→ϕπ0 at the 90% confidence level.
In the developing brain, mature brain derived neurotrophic factor (mBDNF) and its precursor (proBDNF) exhibit prosurvival and proapoptotic functions, respectively. However, it is still unknown whether mBDNF or proBDNF is a major form of neurotrophin expressed in the immature brain, as well as if the level of active caspase-3 correlates with the levels of BDNF forms during normal brain development.

Here we found that both proBDNF and mBDNF were expressed abundantly in the rat brainstem, hippocampus and cerebellum between embryonic day 20 and postnatal day 8. The levels of mature neurotrophin as well as mBDNF to proBDNF ratios negatively correlated with the expression of active caspase-3 across brain regions. The immature cortex was the only structure, in which proBDNF was the major product of bdnf gene, especially in the cortical layers 2-3. And only in the cortex, the expression of BDNF precursor positively correlated with the levels of active caspase-3.

These findings suggest that proBDNF alone may play an important role in the regulation of naturally occurring cell death during cortical development.
The water content in the garnet and clinopyroxene in the mantle eclogites from the V. Grib kimberlite pipe (Arkhangelsk Diamondiferous Province, NW Russia) was analysed using Fourier transform infrared spectrometry.

The results show that all clinopyroxene grains contained structural water at concentrations of 39 to 247 ppm, whereas two garnet samples contained detectable water at concentrations of 211 and 337 ppm. The low-MgO eclogites with oceanic gabbro precursors contained significantly higher water concentrations in the omphacites (70–247 ppm) and whole rock (35–224 ppm) compared to those with oceanic basalt protoliths (49–73 ppm and 20–36 ppm, respectively). The incorporation of water into the clinopyroxene may be associated with vacancies at the M2 site, Al in the tetrahedral position, and the elements that filled the M2 site (mostly Na and Ca).

The highest water content in the omphacite was detected in a nonmetasomatised sample and was assumed to represent residual water that survived during subduction. Other eclogite samples showed signs of modal and/or cryptic metasomatism and contained less water in the omphacites compared to the nonmetasomatised sample. The water content was heterogeneous within the eclogite section of the sampled lithospheric mantle. The lack of distinct and uniform correlations between the indices of eclogite modification and their water content indicated that the saturation with water was disturbed during their residence within the lithospheric mantle.
Over the past several years, there has been an influx of immigrants from Central Asian states into the ranks of “Islamic State” (IS) militants in Syria and Iraq. Those who survived after the defeat of the main detachments of terrorists on their return cannot escape the territory of Afghanistan, the northern regions of which are inhabited by related ethnic groups. It is easy to find supporters of radical Islam in Central Asian countries. The weakness of state and public institutions contributes to the politicization of Islam, especially in the periphery. Islamist preachers, skillfully using the mistakes of local authorities, call for the creation of alternative state structures. Most of the former IS fighters do not hide their intentions to return home. They can gain support in the border provinces of Afghanistan, among their comrades-in-arms in the war, and also join some of the Taliban groups. The planned withdrawal of American troops and their allies from Afghanistan does not yet imply the coming of peace in the region. Therefore, among the main threats to the security of the region are the activities of transnational terrorist groups such as “The Islamic State of Khorasan Province”, “The Islamic Movement of Uzbekistan” and “The Islamic Movement of Eastern Turkestan”.
Lebesgue (1940) proved that every plane triangulation contains a face with the vertex-degrees majorized by one of the following triples: (3,6,∞),(3,7,41),(3,8,23),(3,9,17),(3,10,14),(3,11,13),(4,4,∞),(4,5,19),(4,6,11),(4,7,9),(5,5,9),(5,6,7). Jendrol' (1999) improved this description, except for (4,4,∞) and (4,6,11), to (3,4,35),(3,5,21),(3,6,20),(3,7,16),(3,8,14),(3,9,14),(3,10,13),(4,4,∞),(4,5,13),(4,6,17),(4,7,8),(5,5,7),(5,6,6) and conjectured that the tight description is (3,4,30),(3,5,18),(3,6,20),(3,7,14),(3,8,14),(3,9,12),(3,10,12),(4,4,∞),(4,5,10),(4,6,15),(4,7,7),(5,5,7),(5,6,6). We prove that in fact every plane triangulation contains a face with the vertex-degrees majorized by one of the following triples, where every parameter is tight: (3,4,31),(3,5,21),(3,6,20),(3,7,13),(3,8,14),(3,9,12),(3,10,12),(4,4,∞),(4,5,11),(4,6,10),(4,7,7),(5,5,7),(5,6,6).
In the present paper, we experimentally study the diffusive dynamics in ensembles of self-propelled and self-rotating bristle-bots. Considering the dependence of the system dynamics on the packing density of robots as well as on the friction between individual robots

we show that the friction slightly affects the diffusive dynamics but leads to a significant change in the jamming transition corresponding to the formation of rigid clusters of robots.
We report a search for $X(3872)$ and $X(3915)$ in $B^+ \to \chi_{c1} \pi^0 K^+$ decays.

The results reported here are obtained from $772 \times 10^{6}$ $B\overline{B}$ events collected at the $\Upsilon(4S)$ resonance with the Belle detector at the KEKB asymmetric-energy $e^+e^-$ collider.

We set an upper limit of $\mathcal{B}(B^+ \to X(3872) K^+) \times \mathcal{B}(X(3872) \to \chi_{c1} \pi^0)$ $ < 8.1 \times 10^{-6}$ and $\mathcal{B}(B^+ \to X(3915) K^+) \times \mathcal{B}(X(3915) \to \chi_{c1} \pi^0)$ $ < 3.8 \times 10^{-5}$ at 90\% confidence level. We also measure $\mathcal{B}(X(3872) \to \chi_{c1} \pi^0)/\mathcal{B}(X(3872) \to J/\psi \pi^+ \pi^-) < 0.97$ at 90\% confidence level.
We have performed a tomographic study using a joint data set that includes local and teleseismic events, recorded by a temporary network in the western branch of the East African rift system. From the travel time residuals, we derive a three‐dimensional model of seismic P‐wave velocity anomalies for the crust and upper mantle down to a depth of 80 km. Particular attention is paid to the verification of the inversion results by various resolution tests. The results show that the eastern rift shoulder is characterized by relatively high seismic velocities. Lower velocities are obtained beneath the entire length of the rift valley and the Rwenzori Mountains. A prominent feature is observed north‐east of the mountain range: here we detected a vertically oriented, cylindrical low‐velocity anomaly with maximum amplitudes in the middle crust and the upper mantle lithosphere. We suggest that this anomaly indicates reservoirs of molten material related to the ongoing rifting process within this segment of the rift. Just above this anomaly, at depths between 5 and 16 km, earthquake swarms exist. The observed reduction in P‐wave velocity is used to provide constraints on the possible melt content and temperature anomaly in the uppermost mantle. The observed 3–5% P‐velocity decrease can be explained by melt fraction up to 2%–3.3% or alternatively by a temperature increase of at least 248 to 376 K and even higher‐temperature anomalies are possible if lower ambient temperatures in the reference mantle are assumed. Probably, the two effects act in combination.
Quantitative and qualitative analysis of chromosomal polymorphism in 19 natural populations of Ch.agilis had been performed.

Most studied populations showed a medium level of chromosomal polymorphism: on average 45±3.0% of specimens are heterozygotes with 0.52±0.01 heterozygotic inversion per larvae. Besides inversions, B-chromosomes were found in two populations. The total number of banding sequences found in banding sequence pool of Ch.agilis is 16. Three banding sequences - p'agiB3, p'agiD3, p'agiF3 - are described for the first time.