Hengzongshu/ArticleAgent huggingface dataset:
Some variations of human genome [for example, single nucleotide polymorphisms (SNPs)] are markers of hereditary diseases and drug responses. Analysis of them can help to improve treatment. Computer-based analysis of millions of SNPs in the 1000 Genomes project makes a search for SNP markers more targeted.

Here, we combined two computer-based approaches: DNA sequence analysis and keyword search in databases. In the binding sites for TATA-binding protein (TBP) in human gene promoters, we found candidate SNP markers of gender-biased autoimmune diseases, including rs1143627 [cachexia in rheumatoid arthritis (double prevalence among women)]; rs11557611 [demyelinating diseases (thrice more prevalent among young white women than among non-white individuals)]; rs17231520 and rs569033466 [both: atherosclerosis comorbid with related diseases (double prevalence among women)]; rs563763767 [Hughes syndrome-related thrombosis (lethal during pregnancy)]; rs2814778 [autoimmune diseases (excluding multiple sclerosis and rheumatoid arthritis) underlying hypergammaglobulinemia in women]; rs72661131 and rs562962093 (both: preterm delivery in pregnant diabetic women); and rs35518301, rs34166473, rs34500389, rs33981098, rs33980857, rs397509430, rs34598529, rs33931746, rs281864525, and rs63750953 (all: autoimmune diseases underlying hypergammaglobulinemia in women).

Validation of these predicted candidate SNP markers using the clinical standards may advance personalized medicine.
The reaction of [Cp(Bn) Fe(η(5) -P5 )] (1) (Cp(Bn) =η(5) -C5 (CH2 Ph)5 ) with CuI selectively yields a novel spherical supramolecule (CH2 Cl2 )3.4 @[(Cp(Bn) FeP5 )12 {CuI}54 (MeCN)1.46 ] (2) showing a linkage of the scaffold atoms which is beyond the Fullerene topology.

Its extended CuI framework reveals an outer diameter of 3.7 nm-a size that has not been reached before using five-fold symmetric building blocks. Furthermore, 2 shows a remarkable solubility in CH2 Cl2 , and NMR spectroscopy reveals that the scaffold of the supramolecule remains intact in solution.

In addition, a novel 2D polymer [{Cp(Bn) Fe(η(5) -P5 )}2 {Cu6 (μ-I)2 (μ3 -I)4 }]n (3) with an uncommon structural motif was isolated. Its formation can be avoided by using a large excess of CuI in the reaction with 1.
Современные методы трансгенеза позволяют проводить сложные модификации геномов животных для различных задач. В биотехнологии создают трансгенных животных с модификациями молока, в том числе с улучшенными антибактериальными свойствами, измененным липидным составом или новыми вкусовыми качествами. Одним из перспективных направлений в этой области можно назвать оверэкспрессию β-галактозидаз – ферментов, разлагающих лактозу, – для удаления лактозы из молока сельскохозяйственных животных.

В нашей работе мы исследуем подход с экспрессией трансгалактазилирующего фермента молочнокислой бактерии Lactobacillus delbrueckii. Мы создали две трансгенные линии животных для тестирования этой гипотезы. На первом этапе протестировали различные варианты генетических конструкций, включая сигналы локализации в аппарат Гольджи, и выбрали два варианта гена β-галактозидазы под конститутивным и казеиновым промоторами для получения трансгенных линий мышей методом пронуклеарной микроинъекции. Для каждой из конструкций было получено по четыре трансгенных фаундера.

Мы провели предварительный анализ характеристик трансгенных линий (копийность трансгена, экспрессия, наследование трансгена) и собрали молоко от лактирующих самок для дальнейших исследований.
A large amount of data collected with the CMD-3 detector above the threshold of the nn ̅pair allows to measure the cross section of the process e + e − → nn ̅and the neutron form factor with good accuracy.

This task is complicated by a large number of background events and necessity of finding ways to suppress them.

A technique of identification for e + e − → nn ̅events is described.
Carotid atherosclerosis (CA) and, especially, carotid artery stenosis (CAS), are associated with a high risk of cardiovascular events in subjects with type 2 diabetes (T2D). In this study, we aimed to identify risk factors and biomarkers of subclinical CA and CAS in T2D individuals. High-resolution ultrasonography of carotid arteries was performed in 389 patients. Ninety-five clinical parameters were evaluated, including diabetic complications and comorbidities; antihyperglycemic, hypolipidemic, and antihypertensive therapy; indices of glycemic control and glucose variability (GV); lipid panels; estimated glomerular filtration rate (eGFR); albuminuria; blood cell count; and coagulation. Additionally, serum levels of calponin-1, relaxin, L-citrulline, and matrix metalloproteinase-2 and -3 (MMP-2, -3) were measured by ELISA. In univariate analysis, older age, male sex, diabetes duration, GV, diabetic retinopathy, chronic kidney disease, coronary artery disease, peripheral artery disease, and MMP-3 were associated with subclinical CA. In addition to these factors, long-term arterial hypertension, high daily insulin doses, eGFR, and L-citrulline were associated with CAS. In multivariate logistic regression, age, male sex, BMI, GV, and eGFR predicted CA independently; male sex, BMI, diabetes duration, eGFR, and L-citrulline were predictors of CAS. These results can be used to develop screening and prevention programs for CA and CAS in T2D subjects.
A novel explanation of the evolutionary process leading to the appearance of differences in sexual preferences is proposed. The explanation is fully general: it is not specific to any particular type of sexual preferences, nor to any species or population. It shows how different sexual preferences can appear in any large group-living population in which sexual selection is sufficiently strong in each sex. The main idea is that the lack of interest toward a member of the opposite sex may be interpreted as a signal of popularity, and thus of reproductive success. It is then boosted by the Fisher runaway process far beyond the point where it becomes costly, resulting in a generalized trait-lack of interest toward the opposite sex. If the interest diverts toward other targets then different sexual preferences emerge. This hypothesis is placed into the context of other works on different sexual preferences in animals; supporting evidence from the literature is reviewed and additional research needed to confirm or refute the hypothesis in any given species is outlined.
Intensive evaporation of a thin liquid film, moving in a flat micro-/minichannel under the action of gas flow is very promising for the use in cooling systems of modern semiconductor devices with localized heat sources of high intensity.

In this work, using the high-speed visualization, the effect of the formation of dry spots on heat transfer in a locally heated liquid film shear-driven in a channel was investigated.

It was found that the maximum intensity of heat removal from the heater is achieved in the mode, when the film flow continuity is broken. During the experiment the total area of dry spots increases with increasing heat flux and heater temperature, but when the heater reaches a certain temperature (≈100°C), the total area begins to decrease. However, the length of contact line increases with increasing heat flux and reaches a maximum in the pre-crisis regime. Intensive evaporation in the region of the contact line may explain the achievement of high heat fluxes in the shear-driven liquid film.
Background Pruritus is a major symptom of atopic dermatitis (AD) and is transmitted by a subpopulation of non‐myelinated C‐type free nerve endings in the epidermis and upper dermis. Stimulation of these nerve terminals is affected by histamine, neurotrophins and physical factors. Eosinophils of patients with AD are a source of neurotrophins, including brain‐derived neurotrophic factor (BDNF), levels of which correlate with disease severity.

Objective The purpose of this study was to determine the anatomical localization of eosinophils in the skin of patients with AD with regard to peripheral nerves and to investigate whether eosinophils induce sprouting and neurite outgrowth in murine sensory neurons. Methods Cryosections of skin derived from AD and control (NA) patients were subjected to immunofluorescence analysis with markers for eosinophils, BDNF and neuronal cells. Stimulated eosinophil supernatants were used for the treatment of cultured peripheral mouse dorsal root ganglia (DRG) neurons followed by morphometric analysis.

Results Dermal axon density and the proximity of eosinophils to nerve fibres were significantly higher in AD patients vs NA. Both neuronal projections and eosinophils expressed BDNF. Furthermore, activated eosinophil supernatants induced BDNF‐dependent mouse DRG neuron branching. Conclusions and Clinical Relevance Our results indicate that BDNF‐positive eosinophils are also localized in close proximity with nerve fibres in AD, suggesting a functional relationship between BDNF‐expressing eosinophils and neuronal projections. These observations suggest that eosinophils may have considerable impact on pruritus by supporting sensory nerve branching.
A geometric model of an autostereoscopic 3D display is described herein. This model is based on the geometry of typical display devices and on the projective transformations that provide periodicity in the projective space. The formulas for the transformations between regions and spaces are provided. The model can be applied to the study of an operation of the multiview, integral, plenoptic, and light-field displays. A practical application considered in this paper is the multiview wavelet transform.
The Forced Invariance Method (FIM) is a vegetation suppression algorithm for multispectral images which is of great interest since it uses statistics collected only from the pixels of the image itself. Due to this a priori spectral characteristics of the captured objects are not required.

For using the FIM or its modification in practice it is necessary to have some way to evaluate algorithm efficiency in order to analyze the results of experiments. This paper presents the method designed to solve this problem. The proposed method is based on the idea of comparing a processed image with its standard using the similarity measure.

Presumably, it is suitable for evaluating various algorithms, such as those designed to suppress vegetation.
The human immunodeficiency virus-1 (HIV-1) has the ability to evade the adaptive immune response due to high mutation rates. Soon after the discovery of HIV-1, it was originally proposed that neutralizing of antibodies to the virus occurs rarely or cannot be elicited at all. In the 1990s, there appeared reports that sera of select HIV-1-infected individuals contained antibodies capable of neutralizing different virus subtypes. Such antibodies were named broadly neutralizing antibodies (bNAbs).

Since 2009, the development of new cell technologies has intensified research efforts directed at identifying new bNAbs with a neutralization potency of over 90% of primary HIV-1 isolates. These antibodies have unique characteristics which include high levels of somatic mutations and unusually long variable loops that penetrate through the glycan shield of HIV-1 Env to contact the protein surface.

In this review, we will attempt to summarize the latest data on bNAbs against HIV-1 in terms of their interactions with the sites of vulnerability on HIV-1 glycoproteins.
The paper presents preliminary results of the comparison of the Dictionaries of word associations for French referred further to as FAS-1 and FAS-2, compiled respectively in 2010 and 2020. To check the reliability of their data we used information on word associations provided by an alternative source, the French project Jeuxdemots, which began in 2007.

The first part of the article accounts for dynamic processes in the vocabulary of direct associative dictionaries, focusing on the analysis of the associative field of the adjective malade. To provide a comprehensive comparison, dynamic changes in the inverse vocabulary were also studied. The list of reactions elicited by 50 or more stimuli (so called super-connectors) was analyzed, as well as the list of the most frequent reactions. For this purpose, two special research dictionaries were designed, based on the reactions to 610 identical stimuli.

We established that the psycholinguistic meaning of this item hasn't undergone considerable changes during the last 10 years: most frequent associations are the same, its structure has not changed, small semantic changes in the content of certain categories are not essential and can be neglected. The lists of the first 75 super-connectors in FAS-1 and FAS-2, as well as the most frequent reactions, proved to be almost identical. We only observed an increase in the frequency and number of stimuli which provoked reactions related to the material side of life (namely, work, earning money, studies), and their decrease for reactions related to social life. However, to summarize the major result of the comparative analysis, we may state that the changes in the associative norms of speakers of French are insignificant, being within the statistical margin of error.
We consider thermochemical mantle plumes with thermal power 1.6·1010 W< N <2.7·1010 W (relative thermal power 1.15<Ka<1.9) as plumes with an intermediate thermal power. Such plumes are formed at the core–mantle boundary beneath cratons in the absence of horizontal free‐convection mantle flows beneath them, or in the presence of weak horizontal mantle flows.

A proposed scheme of convection flows in the conduit of a plume with an intermediate thermal power is based on laboratory and theoretical modeling data. A plume ascends (melts out) from the coremantle boundary to critical depth x кр from which magma erupts on the Earth’s surface. The magmatic melt erupts from the plume conduit onto the surface through the eruption conduit. The latter forms under the effect of superlithostatic pressure on the plume roof. While the thickness of the block above the plume roof decreases to a critical value x кр, the shear stress on its cylindrical surface reaches a critical value (strength limit) τкр.Rock fails in the vicinity of the cylindrical block and, as a consequence, the eruption conduit is formed. We estimate the height of the eruption conduit and the time for the plume to ascent to the critical depth x кр. The volume of erupted melt is estimated for kinematic viscosity of melt =0.5–2 м2/с. The depth Δ x from which the melt is transported to the surface is determined. Using the eruption volume, we obtain a relationship between the depth Δ x and the plume conduit diameter for the above‐mentioned kinematic viscosities. The melt flow structure at the plume conduit/eruption conduit interface is determined on the basis of the laboratory modeling data. The photographs of the simulated flow were obtained. The flow line velocities were measured in the main cylindrical conduit (plume conduit) and at the main conduit/eruption conduit interface. A stagnant area is detected in the 'conduit wall/plume roof’ interface zone. The melt flow in the eruption conduit was analyzed as a turbulent flow in the straight cylindrical conduit with diameter d к. According to the experimental modeling and theoretical data, the superlithostatic pressure in the plume conduit is the sum of the frictional pressure drop and the increasing dynamic pressure in the eruption conduit. A relationship between the melt flow velocity in the eruption conduit and superlithostatic pressure has been derived.

In the case that the depth Δ x is larger than 150 km, the melt from the plume conduit can transport diamonds to the Earth’s surface. Thus, the plumes with an intermediate thermal power are diamondiferous.
The axially symmetric mirror trap with deuterium-tritium plasma is considered as a neutron source for the hybrid reactor. The goal of this study is to select the facility parameters, providing the maximum neutron yield at a fixed input power. The procedure is proposed for verifying the sufficient criterion for stability of the drift-cone ion-cyclotron mode. Within a limited set of configurations under consideration, the maximum fusion reaction power of 5.8 MW is achieved at an input power of 100 MW.
A comprehensive and efficient environment and data monitoring system is a vital part of any HEP experiment. In this paper we describe the software web-based framework which is currently used by the CMD-3 Collaboration at the VEPP-2000 Collider and partially by the Muon g-2 experiment at Fermilab to monitor the status of data acquisition and the quality of data taken by the experiments. The system is designed to meet typical requirements and cover various use-cases of DAQ applications, starting from the central configuration, slow control data monitoring, data quality monitoring, user-oriented visualization, control of the hardware and DAQ processes, etc. Being an intermediate middleware between the front-end electronics and the DAQ applications the system is focused to provide a high-level coherent view for shifters and experts for robust operations. In particular, it is used to integrate various experiment dependent monitoring modules and tools into a unified Web oriented portal with appropriate access control policy. The paper describes the design and overall architecture of the system, recent developments and the most important aspects of the framework implementation.
We study numerically the thermoelectricity of the classical Wigner crystal placed in a periodic potential and being in contact with a thermal bath modeled by the Langevin dynamics. At low temperatures the system has sliding and pinned phases with the Aubry transition between them. We show that in the Aubry pinned phase the dimensionless Seebeck coefficient can reach very high values of several hundreds. At the same time the charge and thermal conductivity of the crystal drop significantly inside this phase. Still we find that the largest values of ZT factor are reached in the Aubry phase and for the studied parameter range we obtain . We argue that this system can provide an optimal regime for reaching high ZT factors and realistic modeling of thermoelecriticy. Possible experimental realizations of this model are discussed.
One of remarkable properties of QCD is the gluon Reggeization. The Reggeized gluon is the primary Reggeon in QCD; Pomeron and Odderon appear as compound states of the Reggeized gluons.

Due to negative signature the Reggeized gluon gives the main contributions to high energy QCD amplitudes in each order of perturbation theory. In the leading and next-to-leading logarithmic approximations these amplitudes are given by the Regge pole contributions.

In the next-to-next-to-leading approximation the pole form of the amplitudes is violated by contributions of three-Reggeon cuts. We discuss these contributions to elastic QCD amplitudes.
ABSTRACT Auxin-inducible degron (AID) system is a promising tool for dynamic protein degradation. In mammalian cells, this approach has become indispensable to study fundamental molecular functions, such as replication, chromatin dynamics or transcription, that are otherwise difficult to dissect. We present evaluation of the two prominent AID systems based on OsTIR1 and AtAFB2 auxin receptor F-box proteins (AFBs). We analyzed degradation dynamics of cohesin/condensin complexes subunits in mouse embryonic stem cells (mRad21, mSMC2, mCapH, mCapH2) and human haploid HAP1 line (hRad21, hSMC2). Double antibiotic selection helped to achieve high homozygous AID targeting efficiency for all genes, ranging from 11 to 77%. We found that the main challenge for successful protein degradation is obtaining cell clones with high and stable AFB expression levels due to mosaic expression of AFBs, which also tends to decline with passages in the absence of constant puromycin selection, even at the AAVS1 safe-harbor locus. Comparing two AFBs, we found that OsTIR1 system showed weak dynamics of protein degradation. At the same time, AtAFB2 approach was very efficient even in random integration. Other factors such as degradation dynamics and low basal depletion were also in favor of AtAFB2 system. Our main conclusion is that repeated addition of puromycin to culture medium prevents AtAFB2 silencing and restores auxin sensitivity, facilitating robust protein degradation. We hope that our report will be useful for researchers that plan to establish AID method in their lab.
Low energy p interaction is considered taking into account the polarization of both particles.

The corresponding cross sections are calculated using the Nijmegen nucleon-antinucleon optical potential. Then they are applied to the analysis of the polarization buildup which is due to the interaction of stored antiprotons with polarized protons of a hydrogen target.

It is shown that, at realistic parameters of a storage ring and a target, the filtering mechanism may provide a noticeable beam polarization in a time comparable with the beam lifetime.
Dynamics and direct $CP$ violation in three-body charmless decays of charged $B$ mesons to a proton, an antiproton and a light meson (pion or kaon) are studied using data, corresponding to an integrated luminosity of $1.0\text{ }\text{ }{\mathrm{fb}}^{\ensuremath{-}1}$, collected by the LHCb experiment in $pp$ collisions at a center-of-mass energy of 7 TeV. Production spectra are determined as a function of Dalitz-plot and helicity variables. The forward-backward asymmetry of the light meson in the $p\overline{p}$ rest frame is measured. No significant $CP$ asymmetry in ${B}^{+}\ensuremath{\rightarrow}p\overline{p}{K}^{+}$ decay is found in any region of the Dalitz plane. We present the first observation of the decay ${B}^{+}\ensuremath{\rightarrow}\overline{\ensuremath{\Lambda}}(1520)(\ensuremath{\rightarrow}{K}^{+}\overline{p})p$ near the ${K}^{+}\overline{p}$ threshold and measure $\mathcal{B}({B}^{+}\ensuremath{\rightarrow}\overline{\ensuremath{\Lambda}}(1520)p)=({3.9}_{\ensuremath{-}0.9}^{+1.0}(\mathrm{stat})\ifmmode\pm\else\textpm\fi{}0.1(\mathrm{syst})\ifmmode\pm\else\textpm\fi{}0.3(\mathrm{BF}))\ifmmode\times\else\texttimes\fi{}{10}^{\ensuremath{-}7}$, where BF denotes the uncertainty on secondary branching fractions.
Epilepsy is a common manifestation of mitochondrial disorders, in addition mitochondrial oxidative stress may play an important role in epileptogenesis since it affects the excitability of neurons. Mitochondrial diseases are often difficult to diagnose unless the symptoms are clearly identified as a part of a specific mitochondrial mutation. The use of next-generation sequencing would lead to the rapid identification of genes associated with epilepsy syndromes. In this study, we evaluate the applicability of the NGS method for diagnosis of mitochondrial epilepsy by clinicians and laboratories in Kazakhstan. We performed complete mitochondrial genome sequencing on the Illumina MiSeq platform for 6 patients with epilepsy. Using the MITOMAP and HmtDB databases, we identified three pathogenic variants (MT-ND1 m.3697G> A, m.5628T> C and m.7547T> C) leading to the development of epilepsy, additionally we found 6 variable sites (m.5586C> T, m.10095C> T, 514_515delCA, 16180_16181delAA, C514.CACACA, A955. ACCCC), the clinic of which was not previously mentioned in the literature. Our preliminary study suggests that mitochondrial genes potentially play a role in the pathogenesis of epilepsy and mutations in these genes cause various forms of epilepsy. It is necessary to elucidate the main mechanisms and participation of variants of mtDNA haplogroups in the development of epilepsy to apply the NGS method in diagnosis of mitochondrial epilepsy.