Hengzongshu/ArticleAgent huggingface dataset:
We have made a precise measurement of the absolute branching fractions of Bs->Ds(*)+ Ds(*)- decays using 121.4 fb-1 of data recorded by the Belle experiment running on the Upsilon(5S) resonance.

The results are B(Bs0->Ds+ Ds-) = (0.58 +0.11 -0.09 +-0.13)%, B(Bs0->Ds*+- Ds-+) = (1.8 +-0.2 +-0.4)%, and B(Bs0->Ds*+ Ds*-) = (2.0 +-0.3 +-0.5)%; the sum is B(Bs0->Ds(*)+ Ds(*)- = (4.3 +-0.4 +-1.0)%.

Assuming Bs->Ds(*)+ Ds(*)- saturates decays to CP-even final states, the branching fraction constrains the ratio Delta(Gamma_s)/cos(phi_s), where Delta(Gamma_s) is the difference in widths between the two Bs0-Bs0bar mass eigenstates, and phi_s is the CP-violating phase in Bs0-Bs0bar mixing. For phi_s=0 we obtain Delta(Gamma_s)/Gamma_s = 0.090 +-0.009 (stat.) +-0.023 (syst.), where Gamma_s is the mean decay width. We also measure for the first time the longitudinal polarization fraction of Bs->Ds*+ Ds*-; the result is 0.12 +0.30 -0.28 +0.04 -0.05.
Effect of terahertz radiation (3.6 THz, 81.5 mkm,15 mV) on some behavioral patterns of intact mice has been investigated. In home cage mice demonstrated avoidance of laser ray and enhanced replacement activity in free behavior.

Animals irradiated during 30 minutes manifested an increased level of anxiety, which was evaluated in the plus maze test on the day following the radiation.
Molecular timescales estimate that early animal lineages diverged tens of millions of years before their earliest unequivocal fossil evidence. The Ediacaran macrobiota (~574 to 538 million years ago) are largely eschewed from this debate, primarily due to their extreme phylogenetic uncertainty, but remain germane.

We characterize the development of Charnia masoni and establish the affinity of rangeomorphs, among the oldest and most enigmatic components of the Ediacaran macrobiota. We provide the first direct evidence for the internal interconnected nature of rangeomorphs and show that Charnia was constructed of repeated branches that derived successively from pre-existing branches. We find homology and rationalize morphogenesis between disparate rangeomorph taxa, before producing a phylogenetic analysis, resolving Charnia as a stem-eumetazoan and expanding the anatomical disparity of that group to include a long-extinct bodyplan.

These data bring competing records of early animal evolution into closer agreement, reformulating our understanding of the evolutionary emergence of animal bodyplans.
Hypersonic boundary layer stability and transition were studied experimentally for the test cases of 7 deg half-angle sharp and blunted cones.

The experiments were performed for M=6. Wall pressure pulsations were recorded synchronously with high speed Schlieren visualization. The combined data processing algorithm for coupling of unsteady pressure sensors with high-speed Schlieren images is proposed.

Analysis of the wall pressure pulsations evolution reveals that the turbulent spot may arise in different ways. It was found that for the blunted model the role of the second mode in origination of the turbulent spot is more evident.
A nontrivial smooth steady incompressible Euler flow in three dimensions with compact support is constructed.

Another uncommon property of this solution is the dependence between the Bernoulli function and the pressure.
AIM: The work aimed to reveal structural signs of autophagy in the cytoplasm of isolated hepatocytes in the dynamics of their cultivation. MATERIALS AND METHODS: The cultivated hepatocyte culture cell cycle was studied by flow cytofluorometry. The cells were cultured for 1, 24, and 48 hours. Morphometric analysis was performed using of the computer program Image J. The diameters of the nuclei and cytoplasm of hepatocytes, the volumes of nuclei and cytoplasm, and the nuclear-cytoplasmic ratio were determined. The concentration of intracellular organelles and autophagy was evaluated with magnification by 30000 times. RESULTS: The cell cycle arrest in the G0/G1 stage after 24 hours of hepatocyte cultivation and the preservation of their viability by hour 48 of the experiment without increase in the percentage of cells in the apoptosis stage were revealed. The decrease in the absolute count of cells was registered, as well as an increase in the nuclear-cytoplasmic ratio indicating a decrease in the proportion of hepatocyte cytoplasm in the course of cultivation. After 24 hours of cultivation, autophagosomes with fragments of cytoplasm, glycogen rosettes, and autolysosomes with partially degraded material were revealed in the cell cytoplasm. By hour 48 of the study, a significant decrease in the volume density of glycogen and mitochondria was noted, as well as an increase in basal autophagy in hepatocytes, with a prevalence of glycophagy and mitophagy. CONCLUSIONS: Autophagy maintains cellular homeostasis of isolated hepatocytes under standard culture conditions, as evidenced by a decrease in the volume density of glycogen and mitochondria, and an increase in basal autophagy in the hepatocyte cytoplasm. The findings indicate the contribution of autophagy to the survival of the primary culture of hepatocytes and can be used as an indicator of the adequacy of culturing conditions.
The paper reviews previous and recently obtained geological, stratigraphic and geochronological data on the Russian-Kazakh Altai orogen, which is located in the western Central Asian Orogenic Belt (CAOB), between the Kazakhstan and Siberian continental blocks. The Russian-Kazakh Altai is a typical Pacific-type orogen, which represents a collage of oceanic, accretionary, fore-arc, island-arc and continental margin terranes of different ages separated by strike-slip faults and thrusts.

Evidence for this comes from key indicative rock associations, such as boninite- and turbidite (graywacke)-bearing volcanogenic-sedimentary units, accreted pelagic chert, oceanic islands and plateaus, MORB-OIB-protolith blueschists. The three major tectonic domains of the Russian-Kazakh Altai are: (1) Altai-Mongolian terrane (AMT); (2) subduction-accretionary (Rudny Altai, Gorny Altai) and collisional (Kalba-Narym) terranes; (3) Kurai, Charysh-Terekta, North-East, Irtysh and Char suture-shear zones (SSZ).

The evolution of this orogen proceeded in five major stages: (i) late Neoproterozoic–early Paleozoic subduction-accretion in the Paleo-Asian Ocean; (ii) Ordovician–Silurian passive margin; (iii) Devonian–Carboniferous active margin and collision of AMT with the Siberian continent; (iv) late Paleozoic closure of the PAO and coeval collisional magmatism; (v) Mesozoic post-collisional deformation and anarogenic magmatism, which created the modern structural collage of the Russian-Kazakh Altai orogen. The major still unsolved problem of Altai geology is origin of the Altai-Mongolian terrane (continental versus active margin), age of Altai basement, proportion of juvenile and recycled crust and origin of the middle Paleozoic units of the Gorny Altai and Rudny Altai terranes.
New Cryogenic Avalanche Detector (CRAD) with ultimate sensitivity, that will be able to detect one primary electron released in the cryogenic liquid, is under development in the Laboratory of Cosmology and Particle Physics of the Novosibirsk State University jointly with the Budker Institute of Nuclear Physics. The CRAD will use two sets of cryogenic PMTs in order to get trigger signal either from primary scintillations in liquid Ar or from secondary scintillations in high field gap above the liquid. Two types of cryogenic PMTs produced by Hamamatsu Photonics were tested and the results are presented in this paper. Low background 3 inch PMT R11065- 10 demonstrated excellent performance according to its specifications provided by the producer. The gain measured with single electron response (SER) in liquid Ar reached 10^7, dark count rate rate did not exceed 300 Hz and pulse height resolution of single electron signals was close to 50%(FWHM). However, two R11065-10 PMTs out of 7 tested stopped functioning after several tens minutes of operation immersed completely into liquid Ar. The remaining 5 devices and one R11065-MOD were operated successfully for several hours each with all the parameters according to the producer specifications. Compact 2 inch PMT R6041-506-MOD with metal-channel dynode structure is a candidate for side wall PMT system that will look at electroluminescence in high field region above liquid. Four of these PMTs were tested in liquid Ar and demonstrated gain up to 2x10^7, dark count rate rate below 100 Hz and pulse height resolution of single electron signals of about 110% (FWHM).
A number of modifications to traditional techniques are suggested in order to overcome problems that frequently arise when growing crystals from solution. These improvements, and their combination, help to avoid problems such as poor nucleation, the spontaneous precipitation of many poor-quality small powder-like crystals, crystals adhering to the crystallization vessel or to each other, and chemical degradation of the solution. The proposed techniques can be used to crystallize desirable metastable polymorphs reliably. None of the suggested methods demands the usage of any special or expensive equipment, or specific skills, and they can be implemented in the chemistry curriculum even at secondary school level. Examples are given for the crystallization of small organic molecules such as carboxylic acids, amino acids, pharmaceuticals etc. , but the same techniques are applicable to other classes of compound.
Based on the analysis of the data on the behavior of electric conductivity at the detonation of condensed high explosives (HEs) with the composition CaHbNcOd and the carbon mass fraction higher than 0.1, the conclusion was made of the presence of long carbon nanostructures. These structures penetrate all the space of reacting HE. The structures are formed already in the chemical peak region, and they evolve along the detonation wave.
The multigene family encoding the 5S rRNA, one of the most important structurally-functional part of the large ribosomal subunit, is an obligate component of all eukaryotic genomes. 5S rDNA has long been a favored target for cytological and phylogenetic studies due to the inherent peculiarities of its structural organization, such as the tandem arrays of repetitive units and their high interspecific divergence. The complex polyploid nature of the genome of bread wheat, Triticum aestivum, and the technically difficult task of sequencing clusters of tandem repeats mean that the detailed organization of extended genomic regions containing 5S rRNA genes remains unclear. This is despite the recent progress made in wheat genomic sequencing.

Using pyrosequencing of BAC clones, in this work we studied the organization of two distinct 5S rDNA-tagged regions of the 5BS chromosome of bread wheat. Three BAC-clones containing 5S rDNA were identified in the 5BS chromosome-specific BAC-library of Triticum aestivum. Using the results of pyrosequencing and assembling, we obtained six 5S rDNA- containing contigs with a total length of 140,417 bp, and two sets (pools) of individual 5S rDNA sequences belonging to separate, but closely located genomic regions on the 5BS chromosome.

Both regions are characterized by the presence of approximately 70–80 copies of 5S rDNA, however, they are completely different in their structural organization. The first region contained highly diverged short-type 5S rDNA units that were disrupted by multiple insertions of transposable elements. The second region contained the more conserved long-type 5S rDNA, organized as a single tandem array. FISH using probes specific to both 5S rDNA unit types showed differences in the distribution and intensity of signals on the chromosomes of polyploid wheat species and their diploid progenitors. A detailed structural organization of two closely located 5S rDNA-tagged genomic regions on the 5BS chromosome of bread wheat has been established. These two regions differ in the organization of both 5S rDNA and the neighboring sequences comprised of transposable elements, implying different modes of evolution for these regions.
A calculation of the absorption cross section of some molecules (NH 3 , C 2 H 4 , CO 2 , O 3 , NO 2 , PH 3 , HNO 3 , SF 6 , CH 3 OH, HCOOH, OCS, CH 3 CN, C 2 H 6 , SO 2 , and H 2 O) at the wavelengths transmitted by a CO 2 laser filled with different isotopes ( 12 C 16 O 2 , 13 C 16 O 2 , 12 C 18 O 2 , 14 C 16 O 2 , 14 C 18 O 2 , 13 C 18 O 2 , and 12 C 16 O 18 O) is presented. The spectroscopical parameters for the molecules from GEISA database have been used. Hence the selection of the molecules was substantially based on the availability of the parameters in the database. The results of the calculations may be used in designing the differential absorption technique for remote monitoring of these molecules. The pressure and temperature dependence of the cross sections are described by and coefficients; these coefficients were calculated for the largest absorption cross sections for each molecule. The absorption cross sections of CH 3 OH and HCOOH at low pressures for all these CO 2 lasers are also presented. These calculations are provided for design of new CO 2 -laser-pumped far-infrared lasers.
Flavonoid compounds are secondary plant metabolites, having a functional importance in plant development, protection from pathogens and unfavorable environmental factors. Chalcone synthase (CHS) is a key enzyme in the biosynthesis of flavonoids; it is involved in biosynthesis of all classes of flavonoid compounds. Nevertheless, the Chs gene family in bread wheat (Triticum aestivum L.) has been not characterized yet. The aim of the current study was to investigate structural and functional organization of the Chs genes and evolution of this gene family in bread wheat and relative species. The nucleotide sequences of the eight Chs copies in T. aestivum were identified. Among them, two homoeologous sets of the Chs genes were located on the short (Chs-A1, −B1, −D1) and the long (Chs-A4, −B4, −D4) arms of homoeologous group 2 chromosomes. Two paralogous gene copies in the B-genome (Chs-B2, −B3) were located in the distal regions of 2BS chromosome. To clarify the origin of Chs duplications in the B-genome the phylogenetic analysis with the Chs sequences of Triticum and Aegilops species carrying ancestral genomes was conducted. It was estimated that the first duplication event occurred in the genome of the common ancestor of Triticum and Aegilops genera about 10–12 million years ago (MYA), then another copy was formed in the ancestor of the B-genome about 6–7 MYA. A homology modeling revealed high sequence similarity of bread wheat CHS enzymes. A number of short deletions in coding regions of some Chs sequences are not expected to have any significant functional effects. Estimation of transcriptional activity of the Chs copies along with a comparative analysis of their promoters structure suggested their functional specialization, which likely contributed to the maintaining of the duplicated Chs genes in wheat genome. From possible ten Chs copies in bread wheat genome, eight members of this family retained their intact structure and activity, while two copies appear to be lost at the level of diploid and tetraploid ancestors. Transcriptional assay along with a comparative analysis of the cis-regulatory elements revealed their functional diversification. The multiple functions supported by the Chs family are assumed to be a driving force for duplications of the Chs gene and their retention in plant genome.
The effect of equilibrium contact angle on dynamics of dry spot spreading at disruption of a horizontal water layer heated locally from the substrate was studied using the high-speed Schlieren technique.

Different methods of working surface processing were applied; this allowed variations of the equilibrium contact angle from 27±6° to 74±9° without a change in thermal properties of the system.

It is found out that substrate wettability significantly affects the propagation velocity of dry spot and its final size. It is also found out that the velocity of contact line propagation is higher in the areas of substrate with a higher temperature.
The influence of the parameters of the impulse action on the laminar supersonic flat-plate boundary layer on the excited localized perturbations is investigated at Mach 2.

The influence of the duration of a pulsed discharge on the generated disturbances is studied experimentally. Also, a direct numerical simulation of the influence of the parameters of pulse injection on generated perturbations is carried out.

It is obtained that as the duration of the action on the supersonic boundary layer increases, the amplitude of the generated disturbance increases. The velocity of the propagation downstream of localized disturbances in Mach 2 supersonic flat-plate boundary layer is estimated.
The phenomenological boundary conditions for the envelope wave function, which is applicable for contacts of semiconductors with the rather different crystal symmetry are proposed. It is shown that the boundary conditions are determined by the number of real values, which are independent of the electron energy. The number of these parameters depends on the symmetry of the bordered materials as well as the symmetry of the boundary itself. The proposed boundary conditions are used for the investigation of the light absorption at the indirect-band-gap semiconductor surface. It is shown that the possibility of the electron transitions with the momentum nonconservation could result in enhancement of the absorption. This is especially the case for the small crystallites, which size is about 50 Å, and where the share of the surface atoms is sufficiently large. The influence of the cristallite size as well as the structure of the interface on the absorption are investigated.
As research on sustainable ammonia synthesis via electrochemical and photochemical N2 reduction progresses to include a wider variety of aqueous and aprotic electrolytes, 1H NMR spectroscopy is increasingly adopted as a means for ammonium quantification.

However, this method is highly sensitive to experimental parameters, as demonstrated herein using a highly versatile and robust NMR pulse program. We demonstrate the sensitivity of the measurement to the final pH of the analyzed solution and identify a [H+] concentration range enabling robust quantification. We compare direct quantification versus calibration approaches to show that the former is highly sensitive to spin relaxation effects and identify the latter as the most reliable approach.

This method, when optimized, enables direct, rapid quantification of both 14NH4+ and 15NH4+ within 12–22 min. The limit of detection of 5–10 μM, depending on the solvent, which meets the needs of current electrochemical and photochemical N2 reduction research.
автоматики электрометрии СО РАН, Новосибирск, Россия 2 Новосибирский государственный университет, Новосибирск, Россия 3 Лаборатория фотоники Пермского научного центра УрО РАН, Пермь, Россия ВОЛОКОННЫЙ ИТТЕРБИЕВЫЙ ЛАЗЕР С САМОСКАНИРОВАНИЕМ ЧАСТОТЫ НА ОСНОВЕ ВОЛОКНА С УВЕЛИЧЕННОЙ ПЛОЩАДЬЮ ПОЛЯ МОДЫ Собран и исследован одночастотный волоконный лазер на основе иттербиевого волокна с увеличенной площадью поля моды сердцевины в качестве активной среды с самосканированием частоты в области
Broadband wavelength tuneability can ensure a new level of versatility for laser systems and extend areas of their applications. Principle limitations of achieving wide tuning wavelength ranges are generally defined by the spectral bandwidth of the gain and traditional tuneability techniques, relying on electrically controlled or bulk filters, reducing laser efficiency and generation stability.

In this work, we present nearly 90 nm tuneability in ultrafast Tm-doped fibre laser within the span from 1873 to 1962 nm by implementing variable feedback for efficient control of the excitation level of the active medium and, hence, the gain spectrum. The highest laser efficiency is observed with 20% feedback, generating 580-fs soliton pulses at 1877 nm central wavelength with 1.5 nJ output pulse energy. By combining the nonlinear Shrodinger equation and population inversion rate equations for the gain medium, the developed numerical model helps to unveil nonlinear pulse evolution under the influence of dynamically varying gain spectrum.

The resulting laser system presents a compact and straightforward approach to achieve laser generation with a broad range tuneability of wavelength and operation regimes, which is not impaired by the limitations on laser stability or power performance and can be translated to other wavelength ranges.
We report on the study of localized surface plasmon resonances (LSPRs) arising in arrays of metal (Au) nanoclusters, dimers, and nanoantennas under the influence of external electromagnetic radiation. Using nanolithography, the plasmonic arrays with different morphologies including cylindrical nanoclusters and dimers, linear and H-type nanoantennas were fabricated. Their LSPR frequencies were determined from the analysis of optical reflection and IR spectra. LSPR frequency depends strongly on morphology of metal nanostructures and is varied from visible to terahertz spectral range with variation of the structures from cylindrical nanoclusters to the nanoantennas with a high aspect ratio. We established the interconnection between the structural parameters of the plasmonic arrays and their LSPR frequencies based on the results of 3D electrodynamic simulations.
Currently one of the most popular methods to obtain various molecular co-crystals is mechanochemical synthesis, i.e. mechanical treatment of a mixture of powder reactants. Traditional approach for mechanical treatment is milling in a ball mill or grinding in a mortar. However, using these methods causes a number of problems related to investigation of the reaction's mechanism. For instance, ball mill produces impact treatment and shear treatment simultaneously and it is almost impossible to separate these two types of mechanical treatment.

This study describes how mechanochemical co-crystallization of piroxicam and succinic acid has been investigated by using special model devices constructed for separated impact and shear mechanical treatment. Such devices allowed us to perform controlled impact or shear mechanical treatment, what was necessary for detailed investigation of mechanochemical processes for molecular crystals, based on the X-ray powder diffraction analysis. We could change and control energy and frequency of mechanical pulses in the impact model device and average velocity and pressure in the shear model device.

For some other systems applying controlled impact treatment allowed us to detect the intermediate products of the mechanochemical synthesis of molecular complexes [1-2]. As for `piroxicam – succinic acid" system, a comparison of impact and shear mechanical treatment had lead to opposite results for mechanochemical reactions – shear treatment appeared to disintegrate co-crystal obtained by impact mechanical treatment.
Nanoscale distance measurements by pulse dipolar Electron paramagnetic resonance (EPR) spectroscopy allow new insights into the structure and dynamics of complex biopolymers. EPR detection requires site directed spin labeling (SDSL) of biomolecule(s), which remained challenging for long RNAs up-to-date.

Here, we demonstrate that novel complementary-addressed SDSL approach allows efficient spin labeling and following structural EPR studies of long RNAs. We succeeded to spin-label Hepatitis C Virus RNA internal ribosome entry site consisting of ≈330 nucleotides and having a complicated spatial structure. Application of pulsed double electron–electron resonance provided spin–spin distance distribution, which agrees well with the results of molecular dynamics (MD) calculations.

Thus, novel SDSL approach in conjunction with EPR and MD allows structural studies of long natural RNAs with nanometer resolution and can be applied to systems of biological and biomedical significance.