Hengzongshu/ArticleAgent huggingface dataset:
To analyze differential expression of metalloproteases genes, involved into the processes of stabilization/destabilization of atherosclerotic plaque, with the method of full genome sequencing of RNA, and to evaluate the level of metalloproteases in homogenates of plaques of various types by immune enzyme assay method (IEA).

The study has been conducted on the specimens of atherosclerotic plaques of patients aged 45-65 y.o., inhabitants of Western Siberia with angiographically proven coronary atherosclerosis and no acute coronary syndrome, with stable angina II-IV functional class. Specimens collection from the plaques was done during an operation if there were intraoperational indications. Histology performed. In intima/media homogenates by IEA method, with BCM Diagnostics assays the levels of destructive markers were measured: MMP-1, MMP3, MMP-7, MMP-9, TIMP-1 on the Multiscan EX (Thermo Fisher Scientific, USA). Libraries preparation for full genomic sequencing of RNA was done with Illumina’s TruSeq RNA Sample Preparation Kit (Illumina, USA). Expression profile in tissues was done on HiSeq 1500 (Illumina, USA).

There are differences in expression of the genes MMP2 , MMP7 , MMP8 , MMP9 , MMP12 , и MMP14 in different types of plaques. There was 8 times higher significant raise in increase of the expression level of ММР9 (p<0,001) in unstable plaque of dystrophic-necrotic type. Study by IEA of MMP-7 content, which is an activator of pro-MMP-9, as well as the content of MMP-9 itself, showed their increased levels in unstable plaques comparing to fatty streaks (1,5 and 2,4 times) and young stable plaques (1,4 and 2,1 times). For the gene ММР9 there were significant differences obtained, of expression levels in stable atherosclerotic fibrous plaque and unstable plaque of dystrophic-necrotic type. With the IEA it was found that fatty streaks and young stable atheromas of coronary arteries have an increased concentration of MMP-3 and decreased activity of tissue inhibitor of metalloproteases. In unstable plaques with the tendency to rupture/ulceration there are increased levels of MMP-1, MMP-7, MMP-9.
The elemental composition of 100 oxalate type urinary stones was determined by ICP OES.

The correlation analysis showed relationships between various elements including calcium and sodium, with all correlations being positive.
Least squares collocation method (LSC) is a versatile numerical method for solving boundary value problems for PDE. The present article demonstrates the abilities of LSC to solve various problems – in particular, calculations of bending of isotropic irregular shaped plates and multi-layered anisotropic plates. In order to achieve higher accuracy, new versions of the method utilize high-degree polynomial spaces. The numerical experiments demonstrate high accuracy of the solutions.
We demonstrate the stabilization of two-dimensional nonlinear wave patterns by means of a dissipative confinement potential. Our analytical and numerical analysis, based on the generalized dissipative Gross-Pitaevskii equation, makes use of the close analogy between the dynamics of a Bose-Einstein condensate and that of mode-locked fiber laser, operating in the anomalous dispersion regime. In the last case, the formation of stable two-dimensional patterns corresponds to spatiotemporal mode locking, using dissipation-enhanced mode cleaning. We analyze the main scenarios of pattern destabilization, varying from soliton dissolution to its splitting and spatiotemporal turbulence, and their dependence on graded dissipation.
The work is devoted to the experimental study of the viscosity and rheology of nanofluids based on ethylene glycol with single-walled nanotubes. Polyvinylpyrrolidone is used as a dispersant.

The weight concentration of nanotubes ranged from 0.01 to 1%. In all cases, the weight concentration of the dispersant was controlled. The measurements were carried out in the temperature range from 20 to 40°C.

It was shown that at all weight concentrations the solution of ethylene glycol and dispersant is a Newtonian liquid, and at a maximum concentration (4%) the viscosity of the solution is two and a half times higher than the viscosity of ethylene glycol. At the same time, all the studied nanofluids turned out to be non-Newtonian and have a pseudoplastic character. It was established that ultrasonic treatment of nanofluids leads to partial degradation of the dispersant and a decrease the base fluid viscosity. The degree of this degradation increases with increasing ultrasonic processing power. With the considered low weight concentrations of nanotubes, the dependence of the viscosity coefficient of the nanofluid on temperature is determined by the corresponding dependence of the base fluid.
The main method of preoperative diagnosis of thyroid tumors and the identification of possible metastasis is a cytological examination of smears obtained by fine-needle aspiration biopsy. However, the cytological material of the lymph nodes may not be adequate, and the detection of metastases faces a number of difficulties. In our recent study, we described a variant of the molecular classifier that allows the detection and typing of malignant thyroid tumors by analyzing several molecular markers in cytological preparations.

A total of 86 cytological samples were used, obtained from individual lymph nodes of 62 patients who had a diagnosis – thyroid cancer. Samples were analyzed by real-time polymerase chain reaction regarding the preselected set of molecular markers: the BRAF V600E mutation, the normalized concentration of HMGA2, FN1 and SERPINA1 mRNA, 5 miRNAs and the mitochondrial/nuclear DNA ratio. The decision tree-based classifier was used to discriminate between benign and malignant samples.

The previously described classifier, based on the analysis of the BRAF V600E mutation, the content of HMGA2 mRNA, 3 miRNAs and the mitochondrial/nuclear DNA ratio, revealed metastases of thyroid cancer with good specificity (98 %) but less sensitivity (83 %). Therefore, a new classifier was built, including three markers – HMGA2 and FN1 mRNA, and miRNA-375, which, with regard to the detection of metastases, showed good sensitivity – 93 % with a slight decrease in specificity (up to 96 %). Thus, we demonstrated the possibility of preoperative detection of thyroid cancer metastases in the lymph nodes of the neck lateral cellular tissue by analyzing several molecular markers in cytological material.
The crystal structures of intercalated transition metal dichalcogenides of the type 12R can be characterized by trigonal symmetry as determined by the structure from single crystal X-ray diffraction data [1].Metal atoms of the MS 2 partial structure (M = Nb or Ta) have trigonal prismatic coordination.Cu atoms and additional M atoms may be incorporated on octahedral and tetrahedral sites in the Van der Waals gaps between layers MS 2 .Here we present the structural studies of selected intercalated compounds at different temperatures between 80K and 400K using singlecrystal X-ray diffraction data.The interest in these compounds is the phase transitions related to ordering of the intercalated atoms and charge-density wave transitions at different temperatures.
Quicksort algorithm as an assistant preliminary tool is proposed for acceleration of neighbour search procedure in the frame of molecular dynamics simulations.

Simple estimations are made showing that the number of operations required to determine all the neighbours within a system of particles can be reduced by 2-3 orders of magnitude due to the preliminary sorting.

Test molecular dynamics simulations carried out for virtual crystal structure containing atoms manifest 9-fold acceleration achieved by the modified algorithm.
Specific molecular recognition of γ-cyclodextrin (γ-CD) by the cationic hexanuclear niobium [Nb6 Cl12 (H2 O)6 ]2+ cluster complex in aqueous solutions results in a 1:1 supramolecular assembly {[Nb6 Cl12 (H2 O)6 ]@γ-CD}2+ .

NMR spectroscopy, isothermal titration calorimetry (ITC), and ESI-MS were used to study the interaction between the inorganic cluster and the organic macrocycle. The 1:2 complex was characterized by XRD, elemental analysis, IR spectroscopy, and thermogravimetric analysis (TGA). Quantum chemical calculations were performed to model host-guest interaction.

Such molecular association affects the biological activity of [Nb6 Cl12 (H2 O)6 ]2+ , decreasing its cytotoxicity despite enhanced cellular uptake. The 1:1 stoichiometry is maintained in solution over a large window of the reagents' ratio, but crystallization by slow evaporation produces a 1:2 host-guest complex [Nb6 Cl12 (H2 O)6 @(γ-CD)2 ]Cl2 ⋅20 H2 O featuring the cluster encapsulated between two molecules of γ-CD.
The work is devoted to the study of the regularities of laminar-turbulent transition in supersonic boundary layers. New experimental data are presented on the evolution downstream of natural disturbances in the boundary layer of a flat plate. The experiments were carried out in a low-noise supersonic wind tunnel T-325 of ITAM SB RAS at Mach number M=2.5. The measurements were made by a constant-temperature hot-wire anemometer in the scanning mode. The procedure of decomposition of the fluctuations of the boundary layer into temperature and mass flux disturbances is carried out.
The evolution of the spatial form of the flow versus the Rayleigh number in layers with two rigid horizontal boundaries and layers with a free surface is experimentally investigated depending on the Rayleigh and Marangoni numbers. The experiments were carried out with layers of ethyl alcohol and water. A thermal imager was used to measure temperature fields on free surfaces of liquids.
Modern astrophysical methods of determination of spins of rotating stellar-mass black hole in close binaries and of supermassive black holes in active galactic nuclei are briefly discussed. Effective spins of coalescing binary black holes derived from LIGO/Virgo gravitational wave observations are specially addressed. We consider three types of coalescing binaries: double black holes, black hole-neutron star binaries and primordial double black holes. The effective spins of coalescing astrophysical binary black holes and black holes with neutron stars are calculated for two plausible models of black hole formations from stellar core collapses (without or with additional fallback from the stellar envelope) taking into account the stellar metallicity and star formation rate evolution in the Universe. The calculated distributions do not contradict the reported LIGO/Virgo observations. The effective spins of primordial coalescing stellar-mass black holes can reach a few per cent due to accretion spin-up in the cold external medium.
the quark part of the kernel of the generalized nonforward BFKL equation at nonzero momentum transfer t in the next-to-leading logarithmic approximation

Along with the quark contribution to the gluon Regge trajectory, this part includes pieces coming from the quark-antiquark production and from the quark contribution to the radiative corrections in one-gluon production in Reggeon-Reggeon collisions

The results obtained can be used for an arbitrary representation of the color group in the t channel. Using the results for the adjoint representation, we demonstrate explicitly the fulfillment of the ``bootstrap'' condition for the gluon Reggeization in the next-to-leading logarithmic approximation in the part concerning the quark contribution
We report on the study of magnetophotogalvanic and magnetotransport phenomena in 200 nm partially strained HgTe films. This thickness is slightly larger than the estimated critical thickness of lattice relaxation leaving the film partially relaxed with the value of the energy gap close to zero. We show that illumination of HgTe films with monochromatic terahertz laser radiation results in a giant resonant photocurrent caused by the cyclotron resonance in the surface states. The resonant photocurrent is also detected in the reference fully strained 80 nm HgTe films previously shown to be fully gapped 3D topological insulators. We show that the resonance positions in both types of films almost coincide demonstrating the existence of topologically protected surface states in thick HgTe films. The conclusion is supported by magnetotransport experiments.
A growing body of evidence points to the role of glucose variability (GV) in the development of the microvascular and macrovascular complications of diabetes. In this review, we summarize data on GV-induced biochemical, cellular and molecular events involved in the pathogenesis of diabetic complications. Current data indicate that the deteriorating effect of GV on target organs can be realized through oxidative stress, glycation, chronic low-grade inflammation, endothelial dysfunction, platelet activation, impaired angiogenesis and renal fibrosis. The effects of GV on oxidative stress, inflammation, endothelial dysfunction and hypercoagulability could be aggravated by hypoglycemia, associated with high GV. Oscillating hyperglycemia contributes to beta cell dysfunction, which leads to a further increase in GV and completes the vicious circle. In cells, the GV-induced cytotoxic effect includes mitochondrial dysfunction, endoplasmic reticulum stress and disturbances in autophagic flux, which are accompanied by reduced viability, activation of apoptosis and abnormalities in cell proliferation. These effects are realized through the up- and down-regulation of a large number of genes and the activity of signaling pathways such as PI3K/Akt, NF-κB, MAPK (ERK), JNK and TGF-β/Smad. Epigenetic modifications mediate the postponed effects of glucose fluctuations. The multiple deteriorative effects of GV provide further support for considering it as a therapeutic target in diabetes.
The explosive vaporization of the nanofluid formed by water and SiO2 nanoparticles on a heater with the size of 100×110 μm was studied.

In experiments, the reflected optical signal produced by heater lighting using laser beam was measured. The patterns of the initial stage of the nanofluid explosive vaporization were obtained.

The effect of nanoparticles on the nucleation temperature was discussed. The variation of the nucleation temperature and vaporization time depending on the temperature increase rate was defined.
We consider some implications of the mass defect on the frequency of atomic transitions. We have found that some well-known frequency shifts (such as gravitational and quadratic Doppler shifts) can be interpreted as consequences of the mass defect, i.e., without the need for the concept of time dilation used in special and general relativity theories. Moreover, we show that the inclusion of the mass defect leads to previously unknown shifts for clocks based on trapped ions.
Currently, approximately 3000 species of stick insects are known; however, chromosome numbers, which range between 21 and 88, are known for only a few of these insects. Also, centromere banding staining (C-banding) patterns were described for fewer than 10 species, and fluorescence in situ hybridization (FISH) was applied exclusively in two Leptynia species. Interestingly, 10-25% of stick insects (Phasmatodea) are obligatory or facultative parthenogenetic. As clonal and/or bisexual reproduction can affect chromosomal evolution, stick insect karyotypes need to be studied more intensely.

Chromosome preparation from embryos of five Phasmatodea species (Medauroidea extradentata, Sungaya inexpectata, Sipyloidea sipylus, Phaenopharos khaoyaiensis, and Peruphasma schultei) from four families were studied here by C-banding and FISH applying ribosomal deoxyribonucleic acid (rDNA) and telomeric repeat probes. For three species, data on chromosome numbers and structure were obtained here for the first time, i.e., S. inexpectata, P. khaoyaiensis, and P. schultei.

Large C-positive regions enriched with rDNA were identified in all five studied, distantly related species. Some of these C-positive blocks were enriched for telomeric repeats, as well. Chromosomal evolution of stick insects is characterized by variations in chromosome numbers as well as transposition and amplification of repetitive DNA sequences. Here, the first steps were made towards identification of individual chromosomes in Phasmatodea.
A method for phosphors solid-phase synthesis under high pressure is proposed. Using the method, phosphors based on rare earth garnets were synthesized at a temperature considerably lower (1300 °C) than the temperature of the synthesis by conventional method (above 1650 °C).

Using organic, inorganic ligaments and synthesized phosphors, remote phosphors were created.

The chromaticity coordinates of created LEDs on the base of blue LEDs and remote phosphors fall into the bin of white and green colours. Luminous efficiency reaches 140 lm/W.
Both Disrupted-In-Schizophrenia-1 (DISC1) and dopamine receptors D2R have significant contributions to the pathogenesis of schizophrenia. Our previous study demonstrated that DISC1 binds to D2R and such protein-protein interaction is enhanced in patients with schizophrenia and Disc1-L100P mouse model of schizophrenia (Su et al., 2014). By uncoupling DISC1xD2R interaction (TAT-D2pep), the synthesized TAT-peptide elicited antipsychotic-like effects in pharmacological and genetic animal models, without motor side effects as tardive dyskinesia commonly seen with typical antipsychotic drugs (APD), indicating that the potential of TAT-D2pep of becoming a new APD. Therefore, in the current study, we further explored the APD-associated capacities of TAT-D2pep. We found that TAT-D2pep corrected the disrupted latent inhibition, as a hallmark of schizophrenia associated endophenotype, in Disc1-L100P mutant mice - a genetic model of schizophrenia, supporting further APD' capacity of TAT-D2pep. Moreover, we found that TAT-D2pep elicited nootropic effects in C57BL/6NCrl inbred mice, suggesting that TAT-D2pep acts as a cognitive enhancer, a desirable feature of APDs of the new generation. Namely, TAT-D2pep improved working memory in T-maze, and cognitive flexibility assessed by the latent inhibition paradigm, in C57BL/6N mice. Next, we assessed the impact of TAT-D2pep on hippocampal long-term plasticity (LTP) under basal conditions and upon stimulation of D2 receptors using quinpirole. We found comparable effects of TAT-D2pep and its control TAT-D2pep-scrambled peptide (TAT-D2pep-sc) under basal conditions. However, under stimulation of D2R by quinpirole, LTP was enhanced in hippocampal slices incubated with TAT-D2pep, supporting the notion that TAT-D2pep acts in a dopamine-dependent manner and acts as synaptic enhancer. Overall, our experiments demonstrated implication of DISC1xD2R protein-protein interactions into mechanisms of cognitive and synaptic plasticity, which help to further understand molecular-cellular mechanisms of APD of the next generation.
The review examines the state of knowledge on genes that control the architectonics of wheat plant (spike morphology). It is shown that molecular genetic studies, which have been recently started, allow to find both the orthologous genes from relative species of wheat (barley, rye, etc.) and genes that were not previously used for breeding. Use of these genes for further breeding allows to produce modern wheat commercial cultivars.
the notion of fundamental group of a virtual link introduced by Kauffman

We define a new notion of group of virtual and welded links. We use two approaches for defining it: via representations of (generalized) braids by automorphisms of free groups and via Wirtinger-like labeling on virtual and welded diagrams.

In the case of virtual links our invariant is stronger than the notion of fundamental group of a virtual link introduced by Kauffman. In the case of welded links our invariant coincides with Kauffman invariant, but using a generalization of Wada representations we provide new families of invariants of welded links.
Cuboid diamonds are particularly common in the placers of the northeastern Siberian platform, but their origin remains unclear. These crystals usually range in color from dark yellow to orange and, more interestingly, are characterized by unusual low aggregated nitrogen impurities (non-aggregated C-center), suggesting a short residence time and/or low temperatures at which they have been stored in the mantle. In order to track possible isotopic signature that could help deciphering cuboid diamond’s crystallization processes, δ13C values, δ15N values, and nitrogen concentrations have been determined in situ in three samples using secondary ion mass spectrometry (SIMS), whereas nitrogen aggregation states have been determined by FTIR spectroscopy. The samples fall out of the δ13C vs. δ15N field of canonical mantle composition. Different scales of carbon and nitrogen fractionation may produce the observed variations. Alternatively, mixing mantle and crustal material would obscure initial co-variations of δ13C values with δ15N or nitrogen content.
By using the Gold map, we construct a partition of the hypercube into cosets of Hamming codes such that for every two cosets the corresponding Hamming codes are maximally nonparallel, that is, their intersection cardinality is as small as possible to admit nonintersecting cosets.
The purpose of the study is to research the effect of polymorphism of genes such as CLOCK, ARNTL, PER2, NPAS2, DRD4, DAT, TNF-α, and NPSR1 on sleep disorders in an open population of 25–64-year-old men.

We conducted screening studies of representative samples of men aged 25–64 years. The general examination was carried out according to the standard methods included in the WHO MONICA-Psychosocial Program (MOPSY).

Carriers of the C/T genotype of the CLOCK gene more often than others reported having 'satisfactory' or 'poor' sleep. Carriers of the C/T genotype of the ARNTL gene were more likely to experience anxiety dreams, and they woke up exhausted. Carriers of the A/A genotype of the PER2 gene were more likely to wake up two or more times per night, a total of four to seven times per week. In the population, C/T and T/T genotypes of the NPAS2 gene were significantly more common in individuals with 7-hour sleep. Genotype 4/6 of the DRD4 gene and genotype 9/9 of the DAT gene were significantly associated with sleep disturbances. Carriers of the heterozygous A/G genotype of the TNF-α-308 gene, compared with carriers of all other genotypes, more often rated sleep as 'satisfactory' (30%) than 'good.'
Earlier reports revealed oxysterol metabolites of Opisthorchis spp. liver fluke origin conjugated with DNA bases, suggesting that the generation of these DNA-adducts may underlie the mutagenicity and carcinogenicity of the infection with these food-borne pathogens. Here, we employed liquid chromatography-mass spectrometry to investigate, compare and contrast spectrograms of soluble extracts from Fasciola hepatica adult worms from bile ducts of cattle with those from O. viverrini and O. felineus from experimentally infected hamsters. F. hepatica and Opisthorchis spp. shared common compounds including oxysterol-like metabolites, bile acids and DNA-adducts, but the spectrometric profiles of F. hepatica included far fewer compounds than Opisthorchis species. These findings support the postulate that parasitic oxysterol-like metabolites could be related to carcinogenesis associated to infection and they point to a molecular basis for the differences among major groups of liver flukes concerning infection-induced malignancy.
The number of non-isomorphic n -fold branched coverings of a given closed surface can be determined by the number of nonisomorphic n -fold unbranched coverings of the surface and the number of nonisomorphic connected n -fold graph coverings of a suitable bouquet of circles. A similar enumeration can also be done for regular branched coverings. Some explicit enumerations are also possible.