Hengzongshu/ArticleAgent huggingface dataset:
The article investigates modes of becoming of subject of work of Russian industrial engineers. Based on the data of a qualitative sociological survey of workers at Siberian industrial enterprises (the first series of focused interviews), it was concluded that the main modus for the becoming of engineers as subjects of labor is professional self-improvement through overcoming their specific institutional constraints in working life: outdated equipment, lack of spare parts, non-core tasks and lack of time. A common social practice, in line with which this development is procedurally unfolding, is the design of new products and spare parts largely for the needs of the production itself at the enterprise, rather than for the product market, from improvised, old and used materials and aggregates, which leads to downtime and reduced productivity, and at the structural level is expressed in maintaining the status quo situation in the enterprise – the productive force of the staff is spent on extensive maintenance of production capacity , is used idle – part of it is directed to the development of production, its modernization and increase of qualitative and quantitative indicators of products, the production of new types of products of labor. The situation is characterized by the asynchronous nature of the mechanism of production functioning at enterprises: while improving personal professional qualities at the expense of ingenuity and resourcefulness, engineers, nevertheless, are not able to eliminate the technological lag of production from the needs of industry in the volume and quality of goods. The mechanism of becoming a subject is studied through the prism of a semiotic analysis of the speech practices of the subjects of labor as a discursive designation by engineers of the real sphere of production relations in a language. The institute of labor is considered as an aggregate discourse space about production relations. The subject is represented as originating at the intersection of two main social institutions – language and labor – as becoming subject in the space of shifting and transition “from temptation of the real – through the symbolic – to the phantasmic” and back.
It is of interest to use UV-sulfite based processes to degrade pollutants in wastewater treatment process. In this work, arsenic (As(III)) has been selected as a target pollutant to verify the efficacy of such a hypothesized process. The results showed that As(III) was quickly oxidized by a UV-sulfite system at neutral or alkaline pH and especially at pH 9.5, which can be mainly attributed to the generated oxysulfur radicals. In laser flash photolysis (LFP) experiments (λex = 266 nm), the signals of SO3•− and eaq− generated by photolysis of sulfite at 266 nm were discerned. Quantum yields for photoionization of HSO3− (0.01) and SO32− (0.06) were also measured. It has been established that eaq− does not react with SO32−, but reacts with HSO3− with a rate constant 8 × 107 M−1s−1.
Pre-reforming of propane was studied over an industrial nickel-chromium catalyst under pressures of 1 and 5 bar, at a low steam to carbon molar ratio of 1, in the temperature range of 220–380 °C and at flow rates of 4000 and 12,000 h−1. It was shown that propane conversion proceeded more efficiently at low pressure (1 atm) and temperatures above 350 °C. A genetic algorithm was applied to search for kinetic parameters better fitting experimental results in such a wide range of experimental conditions. Power law and Langmuir–Hinshelwood kinetics were considered. It was shown that only Langmuir–Hinshelwood type kinetics correctly described the experimental data and could be used to simulate the process of propane pre-reforming and predict propane conversion under the given reaction conditions. The significance of Langmuir–Hinshelwood kinetics increases under high pressure and temperatures below 350 °C.
In many late-replicating euchromatic regions of salivary gland polytene chromosomes, DNA is underrepresented. A mutation in the SuUR gene suppresses underreplication and leads to normal levels of DNA polytenization in these regions. We identified the SuUR gene and determined its structure. In the SuUR mutant stock a 6-kb insertion was found in the fourth exon of the gene. A single SuUR transcript is present at all stages of Drosophila development and is most abundant in adult females and embryos. The SuUR gene encodes a protein of 962 amino acids whose putative sequence is similar to the N-terminal part of SNF2/SWI2 proteins. Staining of salivary gland polytene chromosomes with antibodies directed against the SuUR protein shows that the protein is localized mainly in late-replicating regions and in regions of intercalary and pericentric heterochromatin.
Background DNA repair is essential to counteract damage to DNA induced by endo- and exogenous factors, to maintain genome stability. However, challenges to the faithful discrimination between damaged and non-damaged DNA strands do exist, such as mismatched pairs between two regular bases resulting from spontaneous deamination of 5-methylcytosine or DNA polymerase errors during replication. To counteract these mutagenic threats to genome stability, cells evolved the mismatch-specific DNA glycosylases that can recognize and remove regular DNA bases in the mismatched DNA duplexes. The Escherichia coli adenine-DNA glycosylase (MutY/MicA) protects cells against oxidative stress-induced mutagenesis by removing adenine which is mispaired with 7,8-dihydro-8-oxoguanine (8oxoG) in the base excision repair pathway. However, MutY does not discriminate between template and newly synthesized DNA strands. Therefore the ability to remove A from 8oxoG•A mispair, which is generated via misincorporation of an 8-oxo-2′-deoxyguanosine-5′-triphosphate precursor during DNA replication and in which A is the template base, can induce A•T→C•G transversions. Furthermore, it has been demonstrated that human MUTYH, homologous to the bacterial MutY, might be involved in the aberrant processing of ultraviolet (UV) induced DNA damage. Methods Here, we investigated the role of MutY in UV-induced mutagenesis in E. coli . MutY was probed on DNA duplexes containing cyclobutane pyrimidine dimers (CPD) and pyrimidine (6–4) pyrimidone photoproduct (6–4PP). UV irradiation of E. coli induces Save Our Souls (SOS) response characterized by increased production of DNA repair enzymes and mutagenesis. To study the role of MutY in vivo, the mutation frequencies to rifampicin-resistant (Rif R ) after UV irradiation of wild type and mutant E. coli strains were measured. Results We demonstrated that MutY does not excise Adenine when it is paired with CPD and 6–4PP adducts in duplex DNA. At the same time, MutY excises Adenine in A•G and A•8oxoG mispairs. Interestingly, E. coli mutY strains, which have elevated spontaneous mutation rate, exhibited low mutational induction after UV exposure as compared to MutY-proficient strains. However, sequence analysis of Rif R mutants revealed that the frequencies of C→T transitions dramatically increased after UV irradiation in both MutY-proficient and -deficient E. coli strains. Discussion These findings indicate that the bacterial MutY is not involved in the aberrant DNA repair of UV-induced DNA damage.
We present the result of a search for charge asymmetry in B±→J/ψK± decays using 772×106 B¯¯¯B pairs collected at the Υ(4S) resonance by the Belle detector at the KEKB asymmetric-energy e+e− collider.

The CP-violating charge asymmetry is measured

to be ACP(B+→J/ψK+)=[−0.76±0.50 (stat)±0.22 (syst)]%.
Previously, we demonstrated that poorly differentiated cells of various origins, including tumor-initiating stem cells present in the ascites form of mouse cancer cell line Krebs-2, are capable of naturally internalizing both linear double-stranded DNA and circular plasmid DNA.

The method of co-incubating Krebs-2 cells with extracellular plasmid DNA (pUC19) or TAMRA-5'-dUTP-labeled polymerase chain reaction (PCR) product was used. It was found that internalized plasmid DNA isolated from Krebs-2 can be transformed into competent Escherichia coli cells. Thus, the internalization processes taking place in the Krebs-2 cell subpopulation have been analyzed and compared, as assayed by E. coli colony formation assay (plasmid DNA) and cytofluorescence (TAMRA-DNA).

We showed that extracellular DNA both in the form of plasmid DNA and a PCR product is internalized by the same subpopulation of Krebs-2 cells. We found that the saturation threshold for Krebs-2 ascites cells is 0.5 μg DNA/10 6 cells. Supercoiled plasmid DNA, human high-molecular weight DNA, and 500 bp PCR fragments are internalized into the Krebs-2 tumor-initiating stem cells via distinct, non-competing internalization pathways. Under our experimental conditions, each cell may harbor 340–2600 copies of intact plasmid material, or up to 3.097 ± 0.044×10 6 plasmid copies (intact or not), as detected by quantitative PCR. The internalization dynamics of extracellular DNA, copy number of the plasmids taken up by the cells, and competition between different types of double-stranded DNA upon internalization into tumor-initiating stem cells of mouse ascites Krebs-2 have been comprehensively analyzed. Investigation of the extracellular DNA internalization into tumor-initiating stem cells is an important part of understanding their properties and possible destruction mechanisms. For example, a TAMRA-labeled DNA probe may serve as an instrument to develop a target for the therapy of cancer, aiming at elimination of tumor stem cells, as well as developing a straightforward test system for the quantification of poorly differentiated cells, including tumor-initiating stem cells, in the bulk tumor sample (biopsy or surgery specimen).
Cell penetrating peptides (CPP) are promising agents for transporting diverse cargo into the cells. The amino acid sequence and the mechanism of lactaptin entry into the cells allow it to be included into CPP group. Lactaptin, the fragment of human milk kappa-casein, and recombinant lactaptin (RL2) were initially discovered as molecules that induced apoptosis of cultured cancer cells and did not affect non-malignant cells.

Here, we analyzed the recombinant lactaptin potency to form complexes with nucleic acids and to act as a gene delivery system. To study RL2-dependent delivery, three type of nucleic acid were used as a models: plasmid DNA (pDNA), siRNA, and non-coding RNA which allow to detect intracellular localization through their functional activity. We have demonstrated that RL2 formed positively charged noncovalent 110-nm-sized complexes with enhanced green fluorescent protein (EGFP)-expressing plasmid DNA. Ca2+ ions stabilized these complexes, whereas polyanion heparin displaced DNA from the complexes. The functional activity of delivered nucleic acids were assessed by fluorescent microscopy using A549 lung adenocarcinoma cells and A431 epidermoid carcinoma cells. We observed that RL2:pDNA complexes provided EGFP expression in the treated cells and that strongly confirmed the entering pDNA into the cells. The efficiency of cell transformation by these complexes increased when RL2:pDNA ratio increased. Pre-treatment of the cells with anti-RL2 antibodies partly inhibited the entry of pDNA into the cells. RL2-mediated delivery of siRNA against EGFP was analyzed when A549 cells were co-transfected with EGFP-pDNA and RL2:siRNA complexes. siRNA against EGFP efficiently inhibited the expression of EGFP being delivered as RL2:siRNA complexes. We have previously demonstrated that non-coding U25 small nucleolar RNA (snoRNA) can decrease cell viability. Cancer cell transfection with RL2-snoRNA U25 complexes lead to a substantial decrease of cell viability, confirming the efficiency of snoRNA U25 delivery.

Collectively, these findings indicate that recombinant lactaptin is able to deliver noncovalently associated nucleic acids into cancer cells in vitro.
Phytophthora infestans (Mont.) de Bary is one of the main pathogens in the agricultural sector. The most affected are the Solanaceae species, with the potato (Solanum tuberosum) and the tomato (Solanum lycopersicum) being of great agricultural importance. Ornamental Solanaceae can also host the pests Petunia spp., Calibrachoa spp., as well as the wild species Solanum dulcamara, Solanum sarrachoides, etc. Annual crop losses caused by this pathogen are highly significant. Although the interaction between P. infestans and the potato has been investigated for a long time, further studies are still needed.

This review summarises the basic approaches in the fight against the late blight over the past 20 years and includes four sections devoted to methods of control: (1) fungicides; (2) R-gene-based resistance of potato species; (3) RNA interference approaches; (4) other approaches to control P. infestans.

Based on the latest advances, we have provided a description of the significant advantages and disadvantages of each approach.
Exosomes are biological nanovesicles that participate in intercellular communication by transferring biologically active chemical compounds (proteins, microRNA, mRNA, DNA, and others). Due to their small size (diameter 40–100 nm) and high biological compatibility, exosomes are promising delivery tools in personalized therapy. Because artificial exosome synthesis methods are not developed yet, the urgent task is to develop an effective and safe way to obtain exosomes from natural sources.

Milk is the only exosome-containing biological fluid that is commercially available. In this regard, milk exosomes are unique and promising candidates for new therapeutic approaches to treating various diseases, including cancer. The ability of milk exosomes to carry out biologically active substances to the cell makes them promising tools for oral delivery of chemotherapeutic agents.

The appearance of side effects during the use of cytotoxic and cytostatic agents is among the main problems in cancer chemotherapy. According to this, the targeted delivery of chemotherapeutic agents can be a potential solution to the toxic effect of chemotherapy. This review is devoted to the methods of milk exosome isolation, their biological components, and prospects for their use in cancer treatment.
We analyze the one-photon mechanism of $e^+e^-$ pair production in the collision of nonrelativistic nuclei. A comparison is made with the contribution of electric dipole radiation of virtual photon and with the contribution of the two-photon pair production mechanism.

The contribution of electric quadrupole radiation of virtual photon to the corresponding cross section is found. The effect of the finite nuclear size is considered in detail.

It is shown that the contribution of electric quadrupole radiation is dominant in a wide range of relative velocities. The cross section for the production of $e^+e^-$ pair with the capture of an electron by one of the nuclei is also analyzed.
A search for the rare decays of J/psi -> D-S(-) rho(+) + c.c. and J/psi -> D-S(-)rho(+) + c.c.) <(D)over bar(0)<(K)over bar*(0) + c.c.) is performed for the first time using a sample of 225.3 million J/psi events collected with the BESIII detector.

No obvious signal is observed for either of the two decays.

The upper limits on the branching fractions are determined to be B(J/psi -> D-S(-) rho(+) + c.c.) < 1.3 x 10(-5) and B(J/psi -> D-S(-)rho(+) + c.c.) <(D)over bar(0)<(K)over bar*(0) + c.c.) < 2.5 x 10(-6) at the 90% confidence level.
A search for lepton-flavor-violating $Z\to eτ$ and $Z\toμτ$ decays with $pp$ collision data recorded by the ATLAS detector at the LHC is presented. This analysis uses 139 fb$^{-1}$ of Run 2 $pp$ collisions at $\sqrt{s}=13$ TeV and is combined with the results of a similar ATLAS search in the final state in which the $τ$-lepton decays hadronically, using the same data set as well as Run 1 data. The addition of leptonically decaying $τ$-leptons significantly improves the sensitivity reach for $Z\to\ellτ$ decays. The $Z\to\ellτ$ branching fractions are constrained in this analysis to $\mathcal{B}(Z\to eτ)<7.0\times10^{-6}$ and $\mathcal{B}(Z\to μτ)<7.2\times10^{-6}$ at 95% confidence level. The combination with the previously published analyses sets the strongest constraints to date: $\mathcal{B}(Z\to eτ)<5.0\times10^{-6}$ and $\mathcal{B}(Z\to μτ)<6.5\times10^{-6}$ at 95% confidence level.
The paper deals with analysis of the geochemical and NMR characteristics of oils from a number of fields in the West Siberian oil and gas province. The group composition of the studied samples was estimated by NMR data. The correlation with the results of geochemical analysis was established.
This paper aims at the experimental study of the flow characteristics of the ethanol-nitrogen mixture in vertical and horizontal channels.

The 95% ethanol and nitrogen were used as working liquid and gas accordingly in slit rectangular microchannel with cross-section of 200 × 2000 μm and in a circular channel with ID=2.2 mm. The hydraulic focusing and T-shape mixers were used for gas-liquid flow formation. Experimental data were obtained using highspeed visualization, laser scanning, μLIF, and PLIF methods.

Using method μLIF the nonuniform thickness of the liquid film was obtained at the cross-section of the rectangular microchannel. For the circular channel, the measurements of the local film thickness using the PLIF method confirmed accordance of the experimental data with Taylor law.
High-pressure X-ray diffraction was first used to find and solve the structures of high-pressure polymorphs. The challenge today is to find some universal laws and rules of high-pressure transformations and Katrusiak [Acta Cryst. (2019), B75, 918–926] suggests such possible laws and rules.
Empedocles' fragment B17 with the addition of several new lines available in the Strasbourg papyrus (on the basis of the so called Ensemble c)

A commented translation into Russian

This badly fragmented piece of evidence is translated into Russian for the first time
For classes of graph mappings constructed from CH1 -mappings of nilpotent graded groups, we prove area formula on sub-Lorentzian structures of arbitrary depth with multi-dimensional time.
In this paper, differential invariants are used to construct solutions for equations of the dynamics of a viscous heat-conducting gas and the dynamics of a viscous incompressible fluid modified by nanopowder inoculators. To describe the dynamics of a viscous heat-conducting gas, we use the complete system of Navier—Stokes equations with allowance for heat fluxes. Mathematical description of the dynamics of liquid metals under high-energy external influences (laser radiation or plasma flow) includes, in addition to the Navier—Stokes system of an incompressible viscous fluid, also heat fluxes and processes of nonequilibrium crystallization of a deformable fluid. Differentially invariant solutions are a generalization of partially invariant solutions, and their active study for various models of continuous medium mechanics is just beginning. Differentially invariant solutions can also be considered as solutions with differential constraints; therefore, when developing them, the approaches and methods developed by the science schools of academicians N. N. Yanenko and A. F. Sidorov will be actively used. In the construction of partially invariant and differentially invariant solutions, there are overdetermined systems of differential equations that require a compatibility analysis. The algorithms for reducing such systems to involution in a finite number of steps are described by Cartan, Finikov, Kuranishi, and other authors. However, the difficultly foreseeable volume of intermediate calculations complicates their practical application. Therefore, the methods of computer algebra are actively used here, which largely helps in solving this difficult problem. It is proposed to use the constructed exact solutions as tests for formulas, algorithms and their software implementations when developing and creating numerical methods and computational program complexes. This combination of effective numerical methods, capable of solving a wide class of problems, with analytical methods makes it possible to make the results of mathematical modeling more accurate and reliable.
Usually, the emission of low-energy photons in electron-positron (or electron-ion) bunch collisions is calculated with the same approach as for synchrotron radiation (beamstrahlung). However, for soft photons (E_gamma < E_c where E_c is a critical photon energy), when the coherence length of the radiation becomes comparable to the bunch length, the beamstrahlung approximation becomes invalid. In this paper, we present results of our calculation for this region based on approximation of classical currents. We consider several colliders with dense bunches. The number of low-energy photons dN_gamma emitted by N_e electrons per bunch crossing in the energy interval dE_gamma is dN_gamma = alpha g N_e dE_gamma/E_gamma, where alpha is the fine-structure constant, and the function g, which depends on the bunch parameters, typically is of order unity for modern colliders. In particular, for the ILC, we find that E_c = 83 keV and g=5.5 at a vanishing beam axis displacement, and g=0.88, E_c=0.24 keV for KEKB. We also calculate the specific dependence of dN_gamma on the impact parameter between the two beam axes. In principle, the latter aspect allows for online monitoring of the beam axis displacement.
New synthesis technique for the photocatalysts based on Cd1-xMnxS solid solutions was proposed. The inorganic sodium sulfide was used as a sulfur precursor.

The samples were characterized by XRD and TEM techniques. It has been shown that the proposed method results in the formation of the samples which consist of two phases – Mn3O4 and Cd1-xMnxS. The activity of synthesized catalysts was tested in the hydrogen evolution reaction from aqueous suspensions containing Na2S/Na2SO3 salts as electron donors under visible light irradiation (450 nm).

It has been shown that the rate of hydrogen evolution grows with the manganese ratio and significantly exceeds the activity of pristine CdS. The activity of the Cd1-xMnxS/Mn3O4 samples is comparable with the values described for such kind of systems.
Albumins SCA and SAA are short, highly hydrohylic proteins accumulated in large quantity in the cotyledons and seed axes, respectively, of a dry pea ( Pisum sativum L.) seed. SCA was earlier shown to have two allelic variants differing in mobility in polyacrylamide gel electrophoresis in acid medium. Using them the corresponding gene SCA was mapped on Linkage Group V. This gene was used as a useful genetic and phylogeographical marker that still required electrophoretic analysis of the protein while the DNA sequence of the corresponding SCA gene remained unknown. Based the length, the positive charge in acid conditions and the number of lysine residues of SCA and SAA albumins, estimated earlier electrophoretically, the high throughput sequencing data available in public databases were searched for the respective candidates for the corresponding genes SCA and SAA genes among coding sequences residing in the region of the pea genome corresponding to the map position of SCA . Then we sequenced them in a number of pea accessions. Concordance of the earlier electrophoretic data and sequence variation indicated the sequence Psat0s797g0160 of the reference pea genome to be the SCA gene. The sequence Psat0s797g0240 could encode a minor related albumin SA-a2, while a candidate gene for albumin SAA is still missing (as well as electrophoretic variation of both latter albumins). DNA amplification using original primers SCA1_3f and SCA1_3r from genomic DNA and restriction by endonuclease Hind II made SCA a convenient CAPS marker in peas. Thus, the gene encoding the highly hydrophilic albumin SCA accumulated in pea seeds, with its alleles useful classification of pea wild relatives, is now identified in the pea genome and a convenient CAPS marker is worked out on its base.