Hengzongshu/ArticleAgent huggingface dataset:
S2 cells are one of the most widely used Drosophila melanogaster cell lines. A series of studies has shown that they are particularly suitable for RNAi-based screens aimed at the dissection of cellular pathways, including those controlling cell shape and motility, cell metabolism, and host-pathogen interactions. In addition, RNAi in S2 cells has been successfully used to identify many new mitotic genes that are conserved in the higher eukaryotes, and for the analysis of several aspects of the mitotic process. However, no detailed and complete description of S2 cell mitosis at the ultrastructural level has been done. Here, we provide a detailed characterization of all phases of S2 cell mitosis visualized by transmission electron microscopy (TEM).We analyzed by TEM a random sample of 144 cells undergoing mitosis, focusing on intracellular membrane and microtubule (MT) behaviors. This unbiased approach provided a comprehensive ultrastructural view of the dividing cells, and allowed us to discover that S2 cells exhibit a previously uncharacterized behavior of intracellular membranes, involving the formation of a quadruple nuclear membrane in early prometaphase and its disassembly during late prometaphase. After nuclear envelope disassembly, the mitotic apparatus becomes encased by a discontinuous network of endoplasmic reticulum membranes, which associate with mitochondria, presumably to prevent their diffusion into the spindle area. We also observed a peculiar metaphase spindle organization. We found that kinetochores with attached k-fibers are almost invariably associated with lateral MT bundles that can be either interpolar bundles or k-fibers connected to a different kinetochore. This spindle organization is likely to favor chromosome alignment at metaphase and subsequent segregation during anaphase.We discovered several previously unknown features of membrane and MT organization during S2 cell mitosis. The genetic determinants of these mitotic features can now be investigated, for instance by using an RNAi-based approach, which is particularly easy and efficient in S2 cells.
We present a new measurement of the unitarity triangle angle phi3 using a Dalitz plot analysis of the KsPi+Pi- decay of the neutral D meson produced in B->D(*)K decays. The method exploits the interference between D0 and D0bar to extract the angle phi3, strong phase delta and the ratio r of suppressed and allowed amplitudes. We apply this method to a 605 fb-1 data sample collected by the Belle experiment. The analysis uses three decays: B->DK, and B->D*K with D*->DPi0 and D*->Dgamma, as well as the corresponding charge-conjugate modes. From a combined maximum likelihood fit to the three modes, we obtain phi3 = 78.4^+10.8_-11.6 +- 3.6 (syst) +- 8.9 (model) degrees. CP conservation in this process is ruled out at the confidence level (1-CL)=5x10^-4, or 3.5 standard deviations.
The organic matter of the Vendian (Neoproterozoic) Khatyspyt Formation was investigated.

The new data obtained from the GS and GS-MS analyses include: high UCM humps and heightened peaks of steranes and terpanes against the backdrop of C27+ alkanes on chromatograms of saturated hydrocarbons fractions of five samples; predominance of С21-С26 in n-alkanes distribution; the presence of 12- and 13-monomethylalkanes on chromatograms and demethylated terpanes on filtered chromatograms (m/z 191 and 177). Besides, dispersed bitumens with biodegraded hydrocarbons have been for the first time identified in carbonate and carbonate-siliceous rocks of the Khatyspyt Formation. Also 8,14-sekohopanes resistant to biodegradation and gammacerane were established.

The revealed features of the composition and distribution of hydrocarbons showed that their source was the autochthonous (syngeneic) organic matter of the Khatyspyt Formation, one of the potential hydrocarbon source rock of Northeastern Siberia. The composition and content of bitumen are found to be controlled by the inputs of primary organisms (eukaryotes and prokaryotes) which lived in Vendian seas, by changing redox conditions of sediment deposition and transformation, as well as by relatively little effect of temperature and high degree of biological oxidation in hypergenesis. The patterns of bitumen distribution throughout the section show, that besides the overlying deposits, their accumulations can be discovered in the Khatyspyt Formation.
By a proper cover of a finite group G we mean an extension of a nontrivial finite group by G.

Our purpose is to show that a proper cover of a finite simple group L of Lie type always contains an element whose order differs from the element orders of L

provided that the Lie rank of L is sufficiently large.
Lodging is one of the main factors in reducing the yield and grain quality of winter and spring wheat varieties. The resistance of wheat cultivars to lodging largely depends on environmental factors, biological and morphological features of the stem and root systems. Selection of the varieties for resistance to lodging is relevant in many countries of the world and has a number of achievements. Plant height is one of the most important morphological characters associated with lodging resistance. Breeding of the varieties carrying the dwarfing genes ( Rht ) is the main direction to reduce the risk of lodging. The Rht-B1b, Rht-D1b, Rht8 and Rht11 genes are widely used throughout the world due to their significant influence on agronomically valuable traits, including lodging. It turned out to be important to study the anatomical and morphological features and chemical composition of stem tissues, which complement the assessment of resistance to lodging and allow the varietal material to be more fully characterized. The thickness of stem internodes and their anatomical structure play an important role in the stem strength. The diameter of the stem, its thickness and weight, a large number of vascular bundles and a wide ring of mechanical tissues correlate with resistance to lodging. The content of lignin, silicon and cellulose are important structural components and provide the stem strength of wheat plants. Molecular genetic analysis and mapping of genes and quantitative trait loci are of great importance in identifying the genetic basis of the relationship between the anatomical and morphophysiological characters of the stem and root system and lodging. Genetic factors reflecting correlations between the lodging and the thickness of the stem wall, the number of vascular bundles and other characters were mapped to chromosomes 1A, 1B, 2A, 2D, 3A, 4B, 4D, 5A, 5D, 6D and 7D. It has been found that loci with high phenotypic effects on lodging tolerance are colocalized with loci responsible for plant height, stem diameter and stem strength. To increase resistance to lodging, it is necessary to develop a set of agrotechnical methods that reduce the influence of soil and climatic factors and create wheat varieties tolerant to lodging.
Period of active global cybernation and business automation was marked by a problem for Russian employers in the form of a shortage of qualified personnel capable of ensuring the development and competitiveness in world business markets. There was a problem of forming a system for training new human resources that would satisfy the changing economic realities. The Russian Federation had a higher education system as one of the most stable and well-functioning mechanisms for training personnel for all economical sectors until the 90th of the last century, before the country collapse and the beginning of political and economic reforms. An indicator of the system effectiveness was that the graduates of Russian universities were invited to work by well-known corporations from many countries of the world. Over the years of the reforms, Russia has lost its previous model of education, which significantly affects the quality of personnel training today [1]. Many Russian enterprises are forced to follow the path of independent training and specialists retraining. At first, training was organized locally, it was by chance, but Russian entrepreneurs began to adopt foreign experience in forming a knowledge system in a company and even create corporate universities over the time. The study examines preconditions for the system formation and the corporate education market development, provides current models of knowledge accumulation and proposes the author's cognitive model of personnel professional development.
The propagation of X chromosome inactivation is thought to be mediated by the cis-limited spreading of the nonprotein coding Xist transcript. In this report we have investigated the localization of Xist RNA on rodent metaphase chromosomes. We show that Xist RNA exhibits a banded pattern on the inactive X and is excluded from regions of constitutive heterochromatin. The banding pattern suggests a preferential association with gene-rich, G-light regions. Analysis of X:autosome rearrangements revealed that restricted propagation of X inactivation into cis-linked autosomal material is reflected by a corresponding limited spread of Xist RNA. We discuss these results in the context of models for the function of Xist RNA in the propagation of X inactivation.
This article raises the question about job functions and the range of duties performed by a special category of serving people in Siberia called interpreters. The analysis is based on a sample text of the oath intended to a servant, who is appointed to work as an interpreter in the Yakutsk region.

The oath procedure looks as follows: the service person verbally swore honestly faithfully discharge the duties and then had to kiss the cross. This document significantly supplements the traditional range of sources used by historians to study cross-cultural and interlanguage communication as part of the process of colonization of Siberia. The text of oath is dated 1665/1666 and contains a mention of the voivode of the Yakut town.

It clearly demonstrates the full breadth of duties of the Siberian interpreter. Description of his work includes military, fiscal (collecting fur tribute) and intermediary functions in various communicative situations. The role and importance of the translation service appear to be especially vital because of their responsibility for the adequacy of the translation of Russian messages to foreigners and vice versa.
The long history of potato breeding includes the numerous introgressions of resistance genes from many wild species of South and Central America as well as from cultivated species into the breeding genepool. Most R genes belong to the NLR family with nucleotide-binding site–leucine-rich repeat. The aim of this research concerns an evaluation of NLR genes expression in transcriptomes of three potato cultivars (Evraziya, Siverskij, Sudarynya), which combine genetic material from wild and cultivated potato species, and each bears intragenic markers of RB/Rpi-blb1/Rpi-sto1 genes conferring broad-range resistance to late blight. The transcriptomes of the cultivars were compared before and 24 h after the Phytophthora infestans inoculation. The induction of RB/Rpi-blb1/Rpi-sto1 transcript after 24 h of inoculation was detected in the resistant cultivars Siverskij and Sudarynya but not in susceptible cv. Evraziya. This demonstrates the importance of transcriptomic assay for understanding the results of marker-assisted selection and phenotyping. Interestingly, assembling the transcriptomes de novo and analysis with NLR-parser tool revealed significant fractions of novel NLR genes with no homology to the reference genome from 103 (cv. Siverskij) to 160 (S. stoloniferum, 30514/15). Comparison of novel NLRs demonstrated a relatively small intersection between the genotypes that coincided with their complex pedigrees with several interspecific hybridization events. These novel NLRs may facilitate the discovery of new efficient R genes.
Genome-wide association studies (GWAS) comprise a powerful tool for mapping genes of complex traits. However, an inflation of the test statistic can occur because of population substructure or cryptic relatedness, which could cause spurious associations. If information on a large number of genetic markers is available, adjusting the analysis results by using the method of genomic control (GC) is possible. GC was originally proposed to correct the Cochran-Armitage additive trend test. For non-additive models, correction has been shown to depend on allele frequencies. Therefore, usage of GC is limited to situations where allele frequencies of null markers and candidate markers are matched. In this work, we extended the capabilities of the GC method for non-additive models, which allows us to use null markers with arbitrary allele frequencies for GC. Analytical expressions for the inflation of a test statistic describing its dependency on allele frequency and several population parameters were obtained for recessive, dominant, and over-dominant models of inheritance. We proposed a method to estimate these required population parameters. Furthermore, we suggested a GC method based on approximation of the correction coefficient by a polynomial of allele frequency and described procedures to correct the genotypic (two degrees of freedom) test for cases when the model of inheritance is unknown. Statistical properties of the described methods were investigated using simulated and real data. We demonstrated that all considered methods were effective in controlling type 1 error in the presence of genetic substructure. The proposed GC methods can be applied to statistical tests for GWAS with various models of inheritance. All methods developed and tested in this work were implemented using R language as a part of the GenABEL package.
Biological processes are usually distributed over various intracellular compartments. Proteins from diverse cellular compartments are often involved in similar signaling networks. However, the difference in the reaction rates between similar proteins among different compartments is usually quite high. We suggest that the estimation of frequency of intracompartmental as well as intercompartmental protein-protein interactions is an appropriate approach to predict the efficiency of a pathway. Using data from the databases STRING, ANDSystem, IntAct and UniProt, a PPI frequency matrix of intra/inter-compartmental interactions efficiencies was constructed. This matrix included 15 human-specific cellular compartments. An approach for estimating pathway efficiency using the matrix of intra/inter-compartmental PPI frequency, based on analysis of reactions efficiencies distribution was suggested. An investigation of KEGG pathway efficiencies was conducted using the developed method. The clusterization and the ranking of KEGG pathways based on their efficiency were performed. "Amino acid metabolism" and "Genetic information processing" revealed the highest efficiencies among other functional classes of KEGG pathways. "Nervous system" and "Signaling molecules interaction" contained the most inefficient pathways. Statistically significant differences were found between efficiencies of KEGG and randomly-generated pathways. Based on these observations, the validity of this approach was discussed. The estimation of efficiency of signaling networks is a complicated task because of the need for the data on the kinetic reactions. However, the proposed method does not require such data and can be used for preliminary analysis of different protein networks.
This paper is devoted to experimental investigation of the interaction between the pair of precessing vortices in a tangential vortex chamber.

The test section was the tangential vortex chamber with a cylindrical working area. The liquid was tangentially fed into the chamber through 12 rectangular nozzles. The swirl parameter varied in the range 0 ÷ 6.6, and the Reynolds number varied within 6000 ÷ 52000. For the quantitative investigation the optical methods of laser Doppler anemometry (LDA) and the Particle Image Velocimetry (PIV) were used.

On the basis of visualization materials the dependence of the precession frequency of the system of two vortices was obtained. Information about vortices and recirculation zones was obtained from the optical measurements.
A developed tradition of illustrating Siberian texts in Russian literature makes it possible to identify several approaches and corresponding principles for interpreting texts through illustrations. The approach examined in this article can be for convenience called an “icon-like” as it focuses on the Orthodox tradition in visual arts and is related to corresponding iconographic patterns in illustration. One of the key representatives of this approach is an Irkutsk artist Sergey Eloyan, who turned to the art of book illustration in 2003 within a large-scale project by Gennady Sapronov aimed at publishing the most outstanding works by Siberian writers. An edition of The Tsar Fish by Viktor Astafyev ranks significantly in this series. As the book is very complex in terms of genre, the “icon-like” approach became a means for the artist to visualise the liminal chronotope and paradoxical nature of Astafyev’s images when illustrating the piece. Moreover, Eloyan challenged a certain tradition for illustrating Astafyev’s books which complicated the artist’s task. Established in the Soviet period when Astafyev’s works were subjected to severe censorship, that tradition of illustrating did not allow to reveal the fundamental for the author inseparability of the beautiful and unbearable, the substance of life and the substance of death through a set of pictorial imagery. When freed from the shackles of censorship in the post-Soviet period, The Tsar Fish turned out to be saturated with completely polar imagery: the beautiful and disgusting, the high and low do not simply coexist, but merge into something inseparable. It would appear very difficult to even imagine an integral concept of illustration that could translate such a wide range of emotions into static imagery. Since the beginning of the 2000s, however, two attempts have been made to create a systematic pictorial set of images for The Tsar Fish. The first of these are Eloyan’s illustrations, and the second those of Oleg Mikhailov in 2013. Both of these are conceptual interpretations of the task of visualising an artistic text at the junction of traditional and new approaches of book illustration, made from different perspectives.
Objective The aim of the study is to assess changes in heart structure and function associated with heavy alcohol use by comparing echocardiographic indices in a population-based sample to those in patients admitted to an inpatient facility with severe alcohol problems. Methods and results We used data from the Know Your Heart study (2015–2017) which is a cross-sectional study that recruited 2479 participants aged 35–69 years from the general population of the city of Arkhangelsk in Northwest Russia and 278 patients from the Arkhangelsk Regional Psychiatric Hospital with a primary diagnosis related to chronic alcohol use (narcology clinic subsample). The drinking patterns of the population-based sample were characterised in detail. We used regression models controlling for age, sex, smoking, education and waist to hip ratio to evaluate the differences in echocardiographic indices in participants with different drinking patterns. The means of left ventricular end-diastolic diameter and indexed left atrial systolic diameter were increased among heavy drinkers (narcology clinic subsample), while mean left ventricular ejection fraction was decreased in this group compared with the population-based sample. In contrast, the harmful and hazardous drinkers in the population-based sample did not differ from non-problem drinkers with respect to echocardiographic indices of systolic and diastolic function. Conclusions Extremely heavy drinking is associated with a specific set of structural and functional abnormalities of the heart that may be regarded as precursors of alcohol-related dilated cardiomyopathy.
The closed vortex dynamics is considered in the nonrelativistic version of the Abelian Higgs Model.

The effect of the exchange of excitations propagating in the medium on the vortex string motion is taken into account.

The obtained are the effective action and the equation of motion both including the exchange of the propagating excitations between the distant segments of the vortex and the possibility of its interaction with the static fermion asymmetric background. They are applied to the derivation of the time dependence of the basic geometrical contour characteristics.
This paper reports the detection of the myxozoan species Myxobolus elegans Kashkovsky 1966 in common dace (Leuciscus leuciscus) that has not been previously listed as its host. The problem of differentiation of phenotypically similar Myxobolus species is addressed. During parasitological survey of common dace from the desalinated part of the Gulf of Finland at the city of Sestroretsk, Russia, numerous oval-shaped plasmodia, 0.2-0.4 mm in size, filled with Myxobolus spores were found on the gills. Pear-shaped myxospores were 15.4 (14.8-16.0) × 10.2 (9.6-10.9) µm in size with a rib on each valve. On the basis of spore morphology, the species appeared to be similar to M. elegans and Myxobolus hungaricus Jaczó, 1940. In order to identify the species, molecular genetic analysis was performed, and the species was identified on the basis of morphological characteristics and 18S rDNA data. The results obtained indicate that the Myxobolus species observed on the gills of dace is M. elegans. Thus, common dace is another valid host of M. elegans besides the type host, ide (Leuciscus idus).
Aim.To study the association of single-nucleotide polymorphismrs3025058(5а/6а) with the development of stroke in patients of the East Siberian population with cardiovascular pathology and risk factors for its development. Materials and methods.The study involved 260 patients with stroke (age [57.0; 51.062.0]) and 272 patients of the control group (age [55.0; 51.062.0]). Among the patients who underwent stroke, 157 men and 103 women. The control group included 170 men and 102 women. The examination of the main group included: collection of complaints, anamnesis, clinical examination, computed tomography of the brain, electrocardiography, echocardioscopy, ultrasound duplex scanning of the extracranial brachiocephalic arteries, 24-hour monitoring of blood pressure and heart rate, analysis of the blood coagulation system. The patients of the main group had the following cardiovascular pathology and risk factors: arterial hypertension, paroxysmal supraventricular tachycardias, dyslipidemia, atherosclerosis of extracranial brachiocephalic arteries, disorders of the hemostasis system. The control group was examined within the framework of the international project HAPIEE. Molecular genetic research was carried out by real-time PCR. Statistical processing of the material was carried out using the Statistica for Windows 7.0, Excel and SPSS 22 application software. Results.The study established statistically significant associations between the 5a/5a genotype and the 5a allele and stroke in the general group of patients, as well as in the subgroup of men, subgroups of patients with extracranial brachiocephalic arteries atherosclerosis and dyslipidemia. In the subgroup of patients with cardiac arrhythmias, statistically significant results were obtained only for allele 5a, and in the subgroup of women with stroke, subgroups of patients with arterial hypertension and hypercoagulation, no significant associations ofrs3025058(5a/6a) polymorphism with stroke were found. Conclusion.Genotype 5a/5a and allele 5a of the single-nucleotide polymorphismrs3025058(5а/6а) increase the risk of stroke in individuals from the East Siberian population, including those in the presence of such risk factors as extracranial brachiocephalic arteries atherosclerosis and dyslipidemia.
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