Hengzongshu/ArticleAgent huggingface dataset:
The contact Darwin term is demonstrated to be of the same origin as the spin-orbit interaction. The (Zα)4m3/M2 correction to the Lamb shift, generated by the Darwin term, is found for an arbitrary nonvanishing spin of the nucleus, both half-integer and integer. There is also a contribution of the same structure as that of nuclear quadrupole moment. Key Words: Keywords:Darwin termLamb shift
We carried out a directed crystallization of a melt of the following composition (in mol. %): Fe 31.79, Cu 15.94, Ni 1.70, S 50.20, Sn 0.05, As 0.04, Pt, Pd, Rh, Ru, Ag, Au, Se, Te, Bi, and Sb by 0.03. The obtained cylindrical sample consisted of monosulfide solid solution (mss), nonstoichometric isocubanite (icb*), and three modifications of intermediate solid solution (iss1, iss2, iss3) crystallized from the melt.

The simultaneous formation of two types of liquids separated during cooling of the parent sulfide melt was revealed. In the first, concentrations of noble metals associated with Bi, Sb, and Te were found. The second is related to Cu and was found to contain a large amount of S in addition to Bi and Sb.

We established the main types of inclusions formed during fractional crystallization of Pt-bearing sulfide melt. It was shown that noble metals are concentrated in inclusions in the form of RuS2, PdTe2, (Pt,Pd)Te2, PtRhAsS, and Ag2Se, doped with Ag, Cu, and Pd, in mss and in the form of PtAs2; Au-doped with Ag, Cu, and Pd; Ag2Te; and Pd(Bi,Sb)xTe1−x in icb* and iss. As solid solutions in the base metal sulfides, Rh is present in mss, Sn in iss.
Glucose variability (GV) has been recognized recently as a promoter of complications and therapeutic targets in diabetes.

The aim of this study was to reconstruct and analyze gene networks related to GV in diabetes and its complications. For network analysis, we used the ANDSystem that provides automatic network reconstruction and analysis based on text mining. The network of GV consisted of 37 genes/proteins associated with both hyperglycemia and hypoglycemia. Cardiovascular system, pancreas, adipose and muscle tissues, gastrointestinal tract, and kidney were recognized as the loci with the highest expression of GV-related genes. According to Gene Ontology enrichment analysis, these genes are associated with insulin secretion, glucose metabolism, glycogen biosynthesis, gluconeogenesis, MAPK and JAK-STAT cascades, protein kinase B signaling, cell proliferation, nitric oxide biosynthesis, etc.

GV-related genes were found to occupy central positions in the networks of diabetes complications (cardiovascular disease, diabetic nephropathy, retinopathy, and neuropathy) and were associated with response to hypoxia. Gene prioritization analysis identified new gene candidates (THBS1, FN1, HSP90AA1, EGFR, MAPK1, STAT3, TP53, EGF, GSK3B, and PTEN) potentially involved in GV. The results expand the understanding of the molecular mechanisms of the GV phenomenon in diabetes and provide molecular markers and therapeutic targets for future research.
The characteristic time of disappearance of electric field in massive electrodynamics during the capture of electric charge by black holes is calculated. It is shown that this time does not depend upon the photon mass. The electric field at large distances disappears with the speed of light.
To discover sources for novel anti-influenza drugs, we evaluated the antiviral potential of nine extracts from eight medicinal plants and one mushroom (Avena sativa L., Hordeum vulgare Linn. var. nudum Hook. f., Hippophae rhamnoides Linn., Lycium ruthenicum Murr., Nitraria tangutorum Bobr., Nitraria tangutorum Bobr. by-products, Potentilla anserina L., Cladina rangiferina (L.) Nyl., and Armillaria luteo-virens) from the Qinghai–Tibetan plateau against the influenza A/H3N2 virus. Concentrations lower than 125 μg/mL of all extracts demonstrated no significant toxicity in MDCK cells. During screening, seven extracts (A. sativa, H. vulgare, H. rhamnoides, L. ruthenicum, N. tangutorum, C. rangiferina, and A. luteo-virens) exhibited antiviral activity, especially the water-soluble polysaccharide from the fruit body of the mushroom A. luteo-virens. These extracts significantly reduced the infectivity of the human influenza A/H3N2 virus in vitro when used at concentrations of 15.6–125 μg/mL. Two extracts (N. tangutorum by-products and P. anserina) had no A/H3N2 virus inhibitory activity. Notably, the extract obtained from the fruits of N. tangutorum and N. tangutorum by-products exhibited different anti-influenza effects. The results suggest that extracts of A. sativa, H. vulgare, H. rhamnoides, L. ruthenicum, N. tangutorum, C. rangiferina, and A. luteo-virens contain substances with antiviral activity, and may be promising sources of new antiviral drugs.
ABSTRACT Embryo and oocyte cryopreservation is a widely used technology for cryopreservation of genetic resources. One challenging limitation of this technology is the cell damage during freezing associated with the intracellular lipid droplets. We exploit a Raman spectroscopy to investigate the freezing of cumulus-oocyte complexes, mature oocytes and early embryos of a domestic cat. All these cells are rich in lipids. The degree of lipid unsaturation, lipid phase transition from liquid-like disordered to solid-like ordered state (main transition) and triglyceride polymorphic state are studied. For all cells examined, the average degree of lipid unsaturation is estimated about 1.3 (with ±20 % deviation) double bonds per acyl chain. The onset of the main lipid phase transition occurs in a temperature range from −10 to +4 °C and does not depend significantly on the cell type. It is found that lipid droplets in cumulus-oocyte complexes undergo an abrupt lipid crystallization, which not completely correlate with the ordering of lipid molecule acyl chains. In the case of mature oocytes and early embryos obtained in vitro from cumulus-oocyte complexes, the lipid phase transition is broadened. In frozen state lipid droplets inside the cumulus-oocyte complexes have higher content of triglyceride polymorphic β and β′ phases (∼66%) than it is estimated for the mature oocytes and the early embryos (∼50%). For the first time, to our knowledge, temperature evolution of lipid droplets phase state is examined. Raman spectroscopy is proved as a prospective tool for in situ monitoring of lipid phase state in single embryo/oocyte during freezing.
Exosomes are directly involved in governing physiological and pathological conditions of an organism by transferring information (proteins, microRNA, etc.) from producing to receiving cells. We evaluated the influence of protein cargoes of exosomes from plasma, and exosomes from the total blood of healthy females (HFs) and breast cancer patients (BCPs), on crucial steps of tumor progression: EMT, cell proliferation, invasion, cell migration, stimulation of angiogenesis and immune response. We conducted a mass spectrometric analysis of proteomic content of exosomes isolated from samples using ultrafiltration and ultracentrifugation. Their nature has been verified using cryo-electron microscopy and flow cytometry. Bioinformatics analysis showed that 84% of common exosomal proteins were of cytoplasmic and vesicle origin. They perform functions of protein binding and signaling receptor binding, and facilitated the processes of the regulated exocytosis and vesicle-mediated transport. We observed that metabolism-related signaling pathways were enhanced in the exosome-mediated intracellular communication. Half of the identified exosomal proteins from blood of HFs and BCPs are involved in crucial steps of the tumor progression: EMT, cell proliferation, invasion, cell migration stimulation of angiogenesis, and immune response. Moreover, we revealed that proteomic profiles of exosomes from HF total blood were enriched with proteins inhibiting EMT, cell migration, and invasion. Tumor diagnostic/prognostic protein markers accounted for 47% of the total composition of cell-surface-associated exosomes (calculated as the difference between the total blood exosomes and plasma exosomes) from BCP blood. Breast cancer-associated proteins were equally represented in the blood cell-surface-associated exosomes and in the plasma exosomes from BCPs. However, hyper-expressed proteins predominate in the blood cell-surface-associated exosomes as compared to the plasma exosomes (64% vs. 14%). Using breast cancer proteins data from the Human Protein Atlas (HPA) (www.proteinatlas.org/), three favorable (SERPINA1, KRT6B, and SOCS3) and one unfavorable (IGF2R) prognostic protein markers were found in the BCP total blood exosomes. Further studies will be needed to determine whether identified new exosomal proteins from BCP blood are potential breast cancer diagnostic/prognostic markers or novel therapeutic targets.
The article analyses the way the quality of institutions affects the performance of energy saving policies.

Based on the analysis of dynamic panel data for 69 countries over the period from 2002 to 2012, using Arellano-Bond approach, we have shown that the elasticity of energy consumption by price of energy depends on institutional factors. Similar valuations and calculations have been produced for the industrial sector as well as the production sphere.

We also demonstrated that absolute values of the said indicators are higher in OECD than CIS countries over the whole data sample, which is explained by a higher quality of institutions. The energy consumption in the industrial sector has proved to be more sensitive to the quality of institutions than in production sphere as a whole. We have also performed a general analysis of trends of GDP energy consumption for a number of countries pointing out that a growth of energy prices enhances energy saving processes.
This work investigates the dynamics of the contact line during the propagation of a dry spot in a water layer on a solid substrate. The substrate is coated with fluoropolymer by using a hot wire chemical vapor deposition method. The dry spot is generated using a thermocapillary mechanism caused by the heating of the substrate from below by the laser. By analyzing schlieren images, the dependence of the velocity of the contact line during the propagation of a dry spot was obtained for various initial thicknesses of liquid films.
The vapour bubble dynamics during subcooled flow boiling in a flat microchannel with the height of 0.6 mm was investigated using high-speed visualization. Nucleate flow boiling was maintained by localized heat source. The data on the bubble dynamics was compared with well-known classic growth correlation formulas. The obtained data have shown that the bubble size can reach the channel height in about 0.1 ms, which can lead to heat transfer crisis in channels with local heating.
Speech synthesis is widely used in many practical applications. In recent years, speech synthesis technology has developed rapidly. However, one of the reasons why synthetic speech is unnatural is that it often has over-smoothness. In order to improve the naturalness of synthetic speech, we first extract the mel-spectrogram of speech and convert it into a real image, then take the over-smooth mel-spectrogram image as input, and use image-to-image translation Generative Adversarial Networks(GANs) framework to generate a more realistic mel-spectrogram. Finally, the results show that this method greatly reduces the over-smoothness of synthesized speech and is more close to the mel-spectrogram of real speech.
Supramolecular constructions of amphiphilic dendritic molecules are promising vehicles for anti-cancer drug delivery due to the flexibility of their architecture, high drug loading capacity and avoiding off-target effects of a drug.

Herein, we report a new class of amphiphilic dendritic species—triazine-carbosilane dendrons readily self-assembling into pH-sensitive dendrimersomes. The dendrimersomes efficiently encapsulate anticancer drugs doxorubicin and methotrexate.

Chemodrug-loaded dendrimersomes have dose-related cytotoxic activity against leukaemia cell lines 1301 and K562. Our findings suggest that triazine-carbosilane dendrimersomes are prospective drug carriers for anti-cancer therapy.
The blood realizes the transport of substances, which are necessary for livelihoods, throughout the body. The assumption about the relationship some disease and structure of vasculature (in particular of brain) is natural.

In the paper we consider models of Willis' circle for two groups of laboratory mice – one control group and another with diabetes. Vascular net obtained as a result of preprocessing MRI data.

The purpose of the work is to determine the effect of type 1 diabetes on the properties of the laboratory mice vasculature.
There are differences in number and localization of nucleolus organizer regions (NORs) in genomes. In mammalian genomes, NORs are located on autosomes, which are often situated on short arms of acrocentric chromosomes and more rarely in telomeric, pericentromeric, or interstitial regions.

In this work, we report the unique case of active NORs located on gonоsomes of a eutherian mammal, the Javan mouse-deer (Tragulus javanicus). We have investigated the position of NORs by FISH experiments with ribosomal DNA (rDNA) sequences (18S, 5.8S, and 28S) and show the presence of a single NOR site on the X and Y chromosomes. The NOR is localized interstitially on the p-arm of the X chromosome in close proximity with prominent C-positive heterochromatin blocks and in the pericentromeric area of mostly heterochromatic Y.

The NOR sites are active on both the X and Y chromosomes in the studied individual and surrounded by GC enriched heterochromatin. We hypothesize that the surrounding heterochromatin might have played a role in the transfer of NORs from autosomes to sex chromosomes during the karyotype evolution of the Javan mouse-deer.
heterogeneous/biphasic chemical reactions can be monitored with high spectroscopic resolution using zero-field nuclear magnetic resonance spectroscopy. This is possible because magnetic susceptibility broadening is negligible at ultralow magnetic fields.

We show the two-step hydrogenation of dimethyl acetylenedicarboxylate with para-enriched hydrogen gas in conventional glass NMR tubes, as well as in a titanium tube. The low frequency zero-field NMR signals ensure that there is no significant signal attenuation arising from shielding by the electrically conductive sample container.

This method paves the way for in situ monitoring of reactions in complex heterogeneous multiphase systems and in reactors made of conductive materials while maintaining resolution and chemical specificity.
ABSTRACT Reverse transcriptases, RTs, are a standard tool in both fundamental studies and diagnostics used for transcriptome profiling, virus RNA testing and other tasks. RTs should possess elevated temperature optimum, high thermal stability, processivity, and tolerance to contaminants originating from the biological substances under analysis or the purification reagents. Here, we have constructed a set of chimeric RTs, based on the combination of MuLV-RT and DNA-binding domains: the DNA-binding domain of DNA ligase Pyrococcus abyssi and Sto7d protein, Sso7d counterpart, from Sulfolobus tokodaii . Chimeric RTs showed the same optimal temperature and the efficacy of terminal transferase reaction as the original M-MuLV RT. Processivity and the efficiency in cDNA synthesis of the chimeric RT with Sto7d at C-end were increased several-fold. The attachment of Sto7d enhanced the M-MuLV RT tolerance to the most common amplification inhibitors: NaCl, urea, guanidinium chloride, formamide, components of human whole blood, and human blood plasma. Thus, fusing M-MuLV RT with an additional domain resulted in more robust and efficient RTs.
Исследование кристаллических аминокислот в условиях высоких давлений (как фазовых переходов, так и анизотропии деформации структуры в области устойчивости одной и той же фазы) позволяет лучше понять межмолекулярные взаимодействия и внутримолекулярную динамику в данных системах.Кристаллические аминокислоты используются как лекарственные препараты и подвергаются механическим воздействиям при таблетировании.Они рассматриваются как перспективные материалы благодаря нелинейным оптическим и пьезоэлектрическим свойствам.Наконец, кристаллические аминокислоты служат модельными объектами для изучения динамики биополимеров [1; 2].

К настоящему времени исследовано влияние давления на несколько полиморфных модификаций глицина [3-9], Lи DL-формы серина [10-17], L-цистеин [18], L-аланин [19], L-аспарагин [20; 21], DL-валин [17], таурин [22], L-треонин [23], L-цистин [24].

Представляет интерес не только варьировать боковые заместители в молекуле аминокислоты или сопоставлять влияние давле-
This work reports on the study of a new approach to achievement of high-contrast resonant signals from coherent population trapping (CPT) resonances in 87Rb vapour based on feedback control and real-time digital processing of several measured parameters.

This method consists in stabilisation of the value of a function depending on several system parameters measured as the frequency difference of the bichromatic pump radiation is scanned through adjustment of the pumping radiation power with a feedback loop. The present work made use of two such parameters: the pumping radiation power incident on and exiting from the optical cell.

Exploration of the proposed method has shown that stabilisation of a linear combination of these two parameters results in a resonant peak whose contrast exceeds that of regular CPT resonance by more than two orders of magnitude at relatively slow CPT resonance scan rates, (scanning frequency of the frequency difference of the bichromatic field ∼1 Hz). When dynamically exciting the CPT resonance (the scan frequency of the frequency difference of the bichromatic field equal to 2 kHz), the resonant peak contrast was enhanced by over an order of magnitude.
Whole-chromosome fusions play a major role in the karyotypic evolution of reptiles. It has been suggested that certain chromosomes tend to fuse with sex chromosomes more frequently than others. However, the comparative genomic synteny data are too scarce to draw strong conclusions.

We obtained and sequenced chromosome-specific DNA pools of Sceloporus malachiticus, an iguanian species which has experienced many chromosome fusions. To test the hypothesis that the karyotypic shift could be associated with changes in recombination patterns, we performed a synaptonemal complex analysis in this species and in Sceloporus variabilis (2 n = 34).

We found that four of seven lineage-specific fusions involved sex chromosomes, and that certain syntenic blocks which constitute the sex chromosomes, such as the homologues of the Anolis carolinensis chromosomes 11 and 16, are repeatedly involved in sex chromosome formation in different squamate species. It revealed that the sex chromosomes in S. malachiticus had two distal pseudoautosomal regions and a medial differentiated region. We found that multiple fusions little affected the recombination rate in S. malachiticus. Our data confirm more frequent involvement of certain chromosomes in sex chromosome formation, but do not reveal a connection between the gonosome–autosome fusions and the evolution of recombination rate.
In 2017, AWAKE demonstrated the seeded self-modulation (SSM) of a 400 GeV proton beam from the Super Proton Synchrotron at CERN.

The angular distribution of the protons deflected due to SSM is a quantitative measure of the process, which agrees with simulations by the two-dimensional (axisymmetric) particle-in-cell code LCODE to about 5%. The agreement is achieved in beam population scans at two selected plasma densities and in the scan of longitudinal plasma density gradient. The agreement is reached only in the case of a wide enough simulation box (several plasma wavelengths) that is closer to experimental conditions, but requires more computational power.

Therefore, particle-in-cell codes can be used to interpret the SSM physics underlying the experimental data.
Adenovirus vectors are the most frequently used agents for gene therapy, including oncolytic therapy and vaccine development. It’s hard to overestimate the value of adenoviruses during the COVID-19 pandemic as to date four out of four approved viral vector-based SARS-CoV-2 vaccines are developed on adenovirus platform. The vast majority of adenoviral vectors are based on the most studied human adenovirus type 5 (HAdV-C5), however, its immunogenicity often hampers the clinical translation of HAdV-C5 vectors. The search of less seroprevalent adenovirus types led to another species C adenovirus, Adenovirus type 6 (HAdV-C6). HAdV-C6 possesses high oncolytic efficacy against multiple cancer types and remarkable ability to induce the immune response towards carrying antigens. Being genetically very close to HAdV-C5, HAdV-C6 differs from HAdV-C5 in structure of the most abundant capsid protein, hexon. This leads to the ability of HAdV-C6 to evade the uptake by Kupffer cells as well as to distinct opsonization by immunoglobulins and other blood proteins, influencing the overall biodistribution of HAdV-C6 after systemic administration. This review describes the structural features of HAdV-C6, its interaction with liver cells and blood factors, summarizes the previous experiences using HAdV-C6, and provides the rationale behind the use of HAdV-C6 for vaccine and anticancer drugs developments.
The gene of Zinnia elegans L. coding for S-like extracellular ribonuclease (ZRNase II) was used to produce transgenic tobacco plants with an increased ribonuclease activity.

The protein-coding part of ZRNase II included the signal peptide sequence so the transgenic protein was located extracellularly. The cDNA of ZRNase II was cloned under the control of 2'-promoter of the mannopine synthase (MAS 2') gene from Ti-plasmid of Agrobacterium tumefaciens. It was shown that the resultant transgenic plants had an increased ribonuclease activity of the crude extracts and the induction of MAS 2' promoter by wounding additionally increased the activity.

The plants of two transforming lines characterized by different ribonuclease activities were used to analyze the transgene influence on plant resistance to tobacco mosaic virus. The plants demonstrated either absence of disease symptoms or a significant delay in their appearance, depending on the virus content in the inoculum and ribonuclease activity.
New metal-organic frameworks (MOFs) based on zinc and cadmium ions, terephthalic acid, and flexible ligands 1,5-bis(imidazol-1-yl)pentane or 1,6-bis(imidazol-1-yl)hexane were prepared and characterized by X-ray diffraction, thermorgavimetric analysis and IR spectroscopy.

The imidazolyl ligands were prepared by a new robust procedure involving the reaction between imidazole and 1,5-dibromopentane or 1,6-dibromohexane in a superbasic medium (KOH in DMSO). MOFs based on 1,5-bis(imidazol-1-yl)pentane had diamond topology (dia) and are triply interpenetrated. Ligands with longer spacer 1,6-bis(imidazol-1-yl)hexane, terephthalate ions and zinc(II) ions formed five-fold interpenetrated metal-organic framework also with dia topology, while cadmium(II) ions with the same ligands formed eight-connected uninodal net with a very rare self-penetrated topological type ilc and a point symbol 424.5.63.

The influence of the chemical composition of MOFs on their photoluminescent properties is investigated and discussed in detail.