Hengzongshu/ArticleAgent huggingface dataset:
Hepatocellular carcinoma (HCC) is one of the most common malignancies worldwide with limited therapeutic options. Since HCC has been shown to be immunogenic, immunotherapy is considered a promising therapeutic approach. Small interfering RNAs (siRNAs), depending on their structure and sequence, can trigger the innate immune system, which can potentially enhance the adaptive anticancer immune response in the tumor-bearing subjects. Immunostimulatory properties of nucleic acids can be applied to develop adjuvants for HCC treatment.

The transplantable HCC G-29 tumor in male CBA/LacSto (CBA) mice was used to study the effects of immunostimulatory RNA on tumor growth. Tumor size, metastases area in different organs of mice and mouse survival rate were analyzed. Furthermore the mouse serum IFN-α levels were measured using ELISA.

In the present study, we found that a 19-bp RNA duplex (ImmunoStimulattory RNA or isRNA) with 3-nt overhangs at the 3′-ends of specific sequence displays immunostimulatory, antitumor, and antimetastatic activities in mice bearing HCC G-29. Our results demonstrate that isRNA strongly increases the level of interferon-α (IFN-α) by up to 25-fold relative to the level in mice injected with Lipofectamine alone (Mock), and to a lesser extent increases the level of proinflammatory cytokine interleukin-6 (IL-6) (by up to 5.5-fold relative to the Mock level), in mice blood serum. We showed that isRNA reliably (P < 0.05) inhibits primary tumor growth in mice compared to the mock group. Furthermore, injections of isRNA significantly enhanced necrotic processes in the center of the primary tumor, and decreased by twofold the width of the undifferentiated peripheral zone and the number of mitotic cells in this zone. The results showed that isRNA efficiently reduces the area of metastases in the liver, kidneys, and heart of CBA/LacSto mice with HCC. The obtained results clearly demonstrate immunostimulatory and antimetastatic properties of the isRNAs in mice with HCC. Consequently, this short double-stranded RNA can be considered as a potential adjuvant for the therapy of HCC.
Experimental study of the influence of flow pulsation in settling chamber on the supersonic free stream disturbances was carried out. Data on the pulsations in the settling chamber and the efficiency of deturbulization system as well as the correlation of pulsations of the flow of settling chamber and flow pulsations in test section of T-325 supersonic wind tunnel of ITAM SB RAS were obtained.
Effector functions of immunoglobulin G (IgG) are regulated by the composition of a glycan moiety, thus affecting activity of the immune system. Aberrant glycosylation of IgG has been observed in many diseases, but little is understood about the underlying mechanisms.

We performed a genome-wide association study of IgG N-glycosylation (N = 8090) and, using a data-driven network approach, suggested how associated loci form a functional network. We confirmed in vitro that knockdown of IKZF1 decreases the expression of fucosyltransferase FUT8, resulting in increased levels of fucosylated glycans, and suggest that RUNX1 and RUNX3, together with SMARCB1, regulate expression of glycosyltransferase MGAT3.

We also show that variants affecting the expression of genes involved in the regulation of glycoenzymes colocalize with variants affecting risk for inflammatory diseases. This study provides new evidence that variation in key transcription factors coupled with regulatory variation in glycogenes modifies IgG glycosylation and has influence on inflammatory diseases.
The aim of the research was to study the effect of depression, anxiety, and rumination scores on the balance of activity of the default mode network and attention networks revealed in the resting state EEG records. Forty-five healthy volunteers (24 men aged from 18 to 25 years) participated in the resting state EEG recording. The participants filled in the Beck Depression Inventory-II (BDI II), Ruminative Responses Scale, and Eysenck Personality Profiler. The connectivity measures of resting state networks were calculated in EEG data. The networks were detected by the “seed” method. The effects of depressive symptoms, anxiety, and rumination on the connectivity of the networks were analyzed by the regression method. The depressive symptom scores and the rumination scores were correlated with the dominance of the default mode network over attention networks in the right temporal cortex. The depression scores and the anxiety scores were correlated with the dominance of attention networks over the default mode network in the anterior cingulate cortex. It could be suggested that rumination processes are specific for depressive symptoms and are reflected in the dominance of the default mode network in brain structures associated with the processing of emotional introspection. Common to depressive and anxious symptoms is a state of alertness, which is reflected in the dominance of attention networks in brain structures associated with decision-making.
Backgr ound. The basic approach to the production of new common wheat genotypes involving introgressive hybridization entails a long-term process. Doubled haploid production could accelerate it. However, this method is not widely used in breeding programs due to its main limitation: the genotype dependence. Due to genetic differences between wheat and related species, it was assumed that alien genetic materials are different in their capacity to affect androgenesis. The effect of alien translocations on androgenesis has been shown earlier. The aim of this study was to develop a set of DH wheat lines containing a wheat-alien translocation in the genome and study the effect of alien translocations on androgenesis of anther culture in such lines. Materials and methods . The plant material included: the spring wheat cultivar ‘Novosibirskaya 16’, line Velut 991 carrying wheat-alien translocations 1RS.1BL from rye and 5BS.5BL-5SL from Aegilops speltoides Tausch, and four hybrid F3 generation lines (10-7, 14-8, 15-8, 15-12) from their crossing, differing in the content of alien translocations. Results. It was shown that parameters of androgenesis such as the number of embryo-like structures per 100 anthers, the number of albino regenerants per 100 anthers, and the number of green regenerants per 100 anthers varied depending on the line. The best -responding lines Velut 991, 10-7 and 14-8 are characterized by the presence of a 1RS.1BL wheat-rye translocation chromosome. Regeneration frequency of green plants was recorded to be 8,6%, 3,6% and 10,1% respectively. The values of the parameters for lines 15-12 (carrying 5BS.5BL-5SL translocation) and 15-8 (without translocations) did not differ significantly. Conclusion. Therefore, it can be concluded that the presence of the introgressive fragment of chromosome 5S did not affect the efficiency of androgenesis and the short shoulder of chromosome 1R carries genes that stimulated androgenesis in anther culture.
Schizophrenia and autism spectrum disorder (ASD) are multi-factorial and multi-symptomatic psychiatric disorders, each affecting 0.5%–1% of the population worldwide. Both are characterized by impairments in cognitive functions, emotions and behaviour, and they undermine basic human processes of perception and judgment. Despite decades of extensive research, the aetiologies of schizophrenia and ASD are still poorly understood and remain a significant challenge to clinicians and scientists alike. Adding to this unsatisfactory situation, patients with schizophrenia or ASD often develop a variety of peripheral and systemic disturbances, one prominent example of which is cancer, which shows a direct (but sometimes inverse) comorbidity in people affected with schizophrenia and ASD. Cancer is a disease characterized by uncontrolled proliferation of cells, the molecular origin of which derives from mutations of a cell’s DNA sequence. To counteract such mutations and repair damaged DNA, cells are equipped with intricate DNA repair pathways. Oxidative stress, oxidative DNA damage, and deficient repair of oxidative DNA lesions repair have been proposed to contribute to the development of schizophrenia and ASD. In this article, we summarize the current evidence of cancer comorbidity in these brain disorders and discuss the putative roles of oxidative stress, DNA damage and DNA repair in the aetiopathology of schizophrenia and ASD.
Moths of genus Dendrolimus (Lepidoptera: Lasiocampidae) are among the major pests of coniferous forests worldwide. Taxonomy and nomenclature of this genus are not entirely established, and there are many species with a controversial taxonomic position. We present a comparative evolutionary analysis of the most economically important Dendrolimus species in Eurasia. Our analysis was based on the nucleotide sequences of COI and COII mitochondrial genes and ITS2 spacer of nuclear ribosomal genes. All known sequences were extracted from GenBank. Additional 112 new sequences were identified for 28 specimens of D. sibiricus, D. pini, and D. superans from five regions of Siberia and the Russian Far East to be able to compare the disparate data from all previous studies. In total, 528 sequences were used in phylogenetic analysis. Two clusters of closely related species in Dendrolimus were found. The first cluster includes D. pini, D. sibiricus, and D. superans; and the second, D. spectabilis, D. punctatus, and D. tabulaeformis. Species D. houi and D. kikuchii appear to be the most basal in the genus. Genetic difference among the second cluster species is very low in contrast to the first cluster species. Phylogenetic position D. tabulaeformis as a subspecies was supported. It was found that D. sibiricus recently separated from D. superans. Integration of D. sibiricus mitochondrial DNA sequences and the spread of this species to the west of Eurasia have been established as the cause of the unjustified allocation of a new species: D. kilmez. Our study further clarifies taxonomic problems in the genus and gives more complete information on the genetic structure of D. pini, D. sibiricus, and D. superans.
The TAIGA-experiment aims to implement a hybrid detection technique of Extensive Air Showers(EAS) at TeV to PeV energies, combining the wide angle Cherenkov timing array HiSCORE with Imaging Air Cherenkov Telescopes (IACTs). The detector currently consists of 89 HiSCORE stations and two IACTs, distributed over an area of about 1 km².

Our goal is to introduce a new reconstruction technique, combining the good angular and shower core resolution of HiSCORE with the gamma-hadron separation power of the imaging telescopes. With the second IACT in operation, three different event types can be explored: IACT stereo, full hybrid (IACT stereo + stations) and mono hybrid (IACT mono + HiSCORE), the latter being the operational goal of TAIGA.

The status of the development of the full hybrid reconstruction and its verification using real data and simulation are presented.
We study the rare B meson decays B±,0→J/ψK+K−K±,0, B±,0→J/ψϕK±,0, and search for B0→J/ψϕ, using 469×106 B¯B events collected at the Υ(4S) resonance with the BABAR detector at the PEP-II e+e− asymmetric energy collider. We present new measurements of branching fractions and a study of the J/ψϕ mass distribution in search of new charmonium-like states. In addition, we search for the decay B0→J/ψϕ and find no evidence of a signal.1 MoreReceived 31 July 2014DOI:https://doi.org/10.1103/PhysRevD.91.012003© 2015 American Physical Society
The economic growth of Russian regions is one of the key indicators for the country. The regional economies have significant differences in the structure of production and services, therefore, ensuring economic growth depends on various factors of the internal structure and external influence. In this regard, the task of searching for growth factors and risk factors for the sustainable development of Russian regions becomes important.

The aim of this work is to assess the resilience of the economy of energy resource regions of Russia to changes in the fiscal energy policy of the state. The authors found that such factors exert the strongest influence as tax yield and the balance of tax payments. Moreover, the energy resource regions of Russia are resilient to these factors. Considering the tax security of the regional budget, we can say that it has a fairly strong impact. The regional budget deficit and the level of budgetary provision have little impact on the economic growth of oil-producing regions.

From which the authors conclude that the energy regions are resilient to these factors. The regional budget deficit has the smallest impact. After division into clusters, it was revealed that the main oil-producing regions of Russia turned out to be resilient to changes in the fiscal energy policy of the state. Only eight regions of the considered ones turned out to be resilient: Krasnoyarsk Krai, Tyumen Oblast, Astrakhan Oblast, Republic of Sakha, Kaliningrad Oblast, Sakhalin Oblast, Novosibirsk Oblast and Omsk Oblast.
Curvature of quantum wire results in intrasubband absorption of IR radiation that induces stationary photovoltage in presence of circular polarization.

This effect is studied in ballistic (collisionless) and kinetic regimes. The consideration is concentrated on quantum wires with curved central part.

It is shown, that if mean free path is shorter than length of the curved part the photovoltage does not depend on the wire shape, but on the total angle of rotation of wire tangent. It is not the case when mean free path is finite or large. This situation was studied for three specific shapes of wires: 'hard angle', 'open book' and '$\Omega$-like'.
To study the association of mononucleotide polymorphism rs6737848 SOCS5 gene with the risk of development of allergic bronchial asthma.Totally 59 patients studied (19 males, 40 females) with allergic bronchial asthma and 50 healthy people (29 males, 21 females) of controls. All patients underwent clinical and instrumental and laboratory investigations in KICH №20 (Krasnoyarsk city) and molecular-genetic investigation of DNA in the Russia-Italian laboratory "MAGI" (Krasnoyarsk city) and Institution of Internal and Preventive Medicine (Novosibirsk city). Statistics included standard programs: Statistica for Windows 7.0.The results of the study showed statistical predominance of prevalent genotype СС of SOCS5 gene in allergic bronchial asthma patients, comparing to control group.Homozygous genotype of СС gene of SOCS5 is a risk factor for allergic bronchial asthma.
Background: In a previous report, we demonstrated the presence of cells with a neural/glial phenotype on the concave side of the vertebral body growth plate in Idiopathic Scoliosis (IS) and proposed this phenotype alteration as the main etiological factor of IS.In the present study, we utilized the same specimens of vertebral body growth plates removed during surgery for Grade III-IV IS to analyse gene expression.We suggested that phenotype changes observed on the concave side of the vertebral body growth plate can be associated with altered expression of particular genes, which in turn compromise mechanical properties of the concave side.Methods: We used a Real-Time SYBR Green PCR assay to investigate gene expression in vertebral body growth plates removed during surgery for Grade III-IV IS; cartilage tissues from human fetal spine were used as a surrogate control.Special attention was given to genes responsible for growth regulation, chondrocyte differentiation, matrix synthesis, sulfation and transmembrane transport of sulfates.We performed morphological, histochemical, biochemical, and ultrastructural analysis of vertebral body growth plates.Results: Expression of genes that control chondroitin sulfate sulfation and corresponding protein synthesis was significantly lower in scoliotic specimens compared to controls.Biochemical analysis showed 1) a decrease in diffused proteoglycans in the total pool of proteoglycans; 2) a reduced level of their sulfation; 3) a reduction in the amount of chondroitin sulfate coinciding with raising the amount of keratan sulfate; and 4) reduced levels of sulfation on the concave side of the scoliotic deformity. Conclusion:The results suggested that altered expression of genes that control chondroitin sulfate sulfation and corresponding changes in protein synthesis on the concave side of vertebral body growth plates could be causal agents of the scoliotic deformity.
Stress negatively affects processes of synaptic plasticity and is a major risk factor of various psychopathologies such as depression and anxiety. HOMER1 is an important component of the postsynaptic density: constitutively expressed long isoforms HOMER1b and HOMER1c bind to group I metabotropic glutamate receptors MGLUR1 (GRM1) and MGLUR5 and to other effector proteins, thereby forming a postsynaptic protein scaffold. Activation of the GLUR1–HOMER1b,c and/or GLUR5–HOMER1b,c complex regulates activity of the NMDA and AMPA receptors and Ca 2+ homeostasis, thus modulating various types of synaptic plasticity. Dominant negative transcript Homer1a is formed as a result of activity-induced alternative termination of transcription. Expression of this truncated isoform in response to neuronal activation impairs interactions of HOMER1b,c with adaptor proteins, triggers ligand-independent signal transduction through MGLUR1 and/or MGLUR5, leads to suppression of the AMPA- and NMDA-mediated signal transmission, and thereby launches remodeling of the postsynaptic protein scaffold and inhibits long-term potentiation. The studies on animal models confirm that the HOMER1a-dependent remodeling most likely plays an important part in the stress susceptibility, whereas HOMER1a itself can be regarded as a neuroprotector. In this review article, we consider the effects of different stressors in various animal models on HOMER1 expression as well as impact of different HOMER1 variants on human behavior as well as structural and functional characteristics of the brain.
Aim. To assess the outcomes of myocardial revascularization (MR) and identify risk factors for early postoperative complications in patients with coronary artery disease (CAD) with acute coronary syndrome (ACS) in the context of coronavirus disease 2019 (COVID-19) pandemic. Material and methods . The study included 769 patients aged 67,0±4,4 years with CAD with ACS in the period from April to October 2020. In an expedited manner, percutaneous transluminal coronary angioplasty (n=699) and on pump coronary artery bypass grafting (CABG) (n=70) were performed. All patients underwent a COVID-19 rapid tests. After MR, the following outcomes were recorded: adverse cardiovascular events and other complications; various surgical interventions; bilateral COVID-19 pneumonia; death. The follow-up period lasted 30 days. Results. During the hospitalization, COVID-19 was detected in 5,3% of patients (n=41). Among them, bilateral multisegmental pneumonia developed in 48,8%. Among infected patients, COVID-19-related mortality in the early postoperative period was 9,8%. The all-cause mortality rate was 0,7%. On pump CABG significantly increases the risk of developing COVID-19 pneumonia (odds ratio (OR), 23,2; 95% confidence interval (CI) 14,2-35,4; p<0,001). After MR, COVID-19 pneumonia was associated with respiratory (OR, 7,6; 95% CI, 4,3-11,5; p=0,001) and heart failure (OR, 4,2; 95% CI, 2,9-8,6; p=0,001), atrial fibrillation (OR, 8,3; 95% CI, 4,1-13,9; p=0,001), as well as with all-cause mortality (OR, 10,3; 95% CI, 5,2-16,7; p=0,005). Recurrent transmural myocardial infarction in patients with CAD was associated with heart failure after MR (OR, 7,1; 95% CI, 2,4-12,6; p=0,012). Conclusion. Conducting on pump CABG in patients with CAD with ACS is the leading trigger for developing COVID-19 pneumonia, which, during hospitalization after MR, was associated not only with respiratory complications, but also with impaired heart function, which significantly increases the death risk in this category of patients.
It is believed that Fe7C3 carbide may be a component of the Earth’s inner core.

The paper presents the results of experimental studies on the synthesis of carbide Fe7C3 at 5,5 GPa. It was found that carbide Fe3C and several polytypes of carbide Fe7C3 are formed together with diamond when the system is cooled.

The obtained results indicate that carbide Fe7C3 in the form of polytypic modifications under natural conditions could be formed at relatively low pressures at the stage of differentiation of the Earth.
Small hairpin RNAs (shRNAs) became an important research tool in cell biology. Reliable design of these molecules is essential for the needs of large functional genomics projects.

To optimize the design of efficient shRNAs, we performed comparative, thermodynamic, and correlation analyses of ~18,000 miR30-based shRNAs with known functional efficiencies, derived from the Sensor Assay project (Fellmann et al., 2011). We identified features of the shRNA guide strand that significantly correlate with the silencing efficiency and performed multiple regression analysis, using 4/5 of the data for training purposes and 1/5 for cross validation. Software "miR_Scan" was developed based upon the optimized models.

A model that included the position-dependent nucleotide preferences was predictive in the cross-validation data subset (R = 0.39). However, a model, which in addition to the nucleotide preferences included thermodynamic shRNA features such as a thermodynamic duplex stability and position-dependent thermodynamic profile (dinucleotide free energy) was performing better (R = 0.53). Calculated mRNA target secondary structure stability showed correlation with shRNA silencing efficiency but failed to improve the model. Correlation analysis demonstrates that our algorithm for identification of efficient miR30-based shRNA molecules performs better than approaches that were developed for design of chemically synthesized siRNAs (R(max) = 0.36).
We discuss recent astronomical data showing that the role of massive primordial black holes in the Universe is much more significant than previously thought, both for the present Universe and for redshifts of the order of 10.

We consider the model proposed in 1993 accounting for the primordial creation of heavy and superheavy black holes with a log-normal mass spectrum,

which naturally explains some facts about the Universe that are unaccounted for in standard cosmology and astrophysics.
We study the total cross section for the collision of two highly-virtual photons at large energies, taking into account the BFKL resummation of energy logarithms with full next-to-leading accuracy. A necessary ingredient of the calculation, the next-to-leading order impact factor for the photon to photon transition, has been calculated by Balitsky and Chirilli using an approach based on the operator expansion in Wilson lines.

We extracted the result for the photon impact factor in the original BFKL calculation scheme comparing the expression for the photon-photon total cross section obtained in BFKL with the one recently derived by Chirilli and Kovchegov in the Wilson-line operator expansion scheme. We perform a detailed numerical analysis, combining different, but equivalent in nextto-leading accuracy, representations of the cross section with various optimization methods of the perturbative series.

We compare our results with previous determinations in the literature and with the LEP2 experimental data. We find that the account of Balitsky and Chirilli expression for the photon impact factor reduces the BFKL contribution to the cross section to very small values, making it impossible to describe LEP2 data as the sum of BFKL and leading-order QED quark box contributions.