Hengzongshu/ArticleAgent huggingface dataset:
Preeclampsia is one of the most severe gestational complications which is one of the leading causes of maternal and perinatal morbidity and mortality. A growth in the incidence of severe and combined forms of the pathology has been observed in recent years. According to modern concepts, inadequate cytotrophoblast invasion into the spiral arteries of the uterus and development of the ischemia-reperfusion syndrome in the placental tissue play the leading role in the development of preeclampsia, which is characterized by multipleorgan failure.

In this regard, our work was aimed at studying the patterns of placental tissue transcriptome that are specific to females with PE and with physiological pregnancy, as well as identifying the potential promising biomarkers and molecular mechanisms of this pathology. We have identified 63 genes whose expression proved to differ significantly in the placental tissue of females with PE and with physiological pregnancy.

A cluster of differentially expressed genes (DEG) whose expression level is increased in patients with preeclampsia includes not only the known candidate genes that have been identified in many other genome-wide studies (e.g., LEP, BHLHB2, SIGLEC6, RDH13, BCL6), but also new genes (ANKRD37, SYDE1, CYBA, ITGB2, etc.), which can be considered as new biological markers of preeclampsia and are of further interest. The results of a functional annotation of DEG show that the development of preeclampsia may be related to a stress response, immune processes, the regulation of cell-cell interactions, intracellular signaling cascades, etc. In addition, the features of the differential gene expression depending on preeclampsia severity were revealed. We have found evidence of the important role of the molecular mechanisms responsible for the failure of immunological tolerance and initiation of the pro-inflammatory cascade in the development of severe preeclampsia. The results obtained elaborate the concept of the pathophysiology of preeclampsia and contain the information necessary to work out measures for targeted therapy of this disease.
Massively parallel reporter assays (MPRAs) enable high-throughput functional evaluation of numerous DNA regulatory elements and/or their mutant variants. The assays are based on the construction of reporter plasmid libraries containing two variable parts, a region of interest (ROI) and a barcode (BC), located outside and within the transcription unit, respectively. Importantly, each plasmid molecule in a such a highly diverse library is characterized by a unique BC–ROI association.

The reporter constructs are delivered to target cells and expression of BCs at the transcript level is assayed by RT-PCR followed by next-generation sequencing (NGS). The obtained values are normalized to the abundance of BCs in the plasmid DNA sample. However, depending on the MPRA library construction design, the BC and ROI sequences as well as their associations can be a priori unknown. In such a case, the BC and ROI sequences, their possible mutant variants, and unambiguous BC–ROI associations have to be identified, whereas all uncertain cases have to be excluded from the analysis. Besides the preparation of additional 'mapping' samples for NGS, this also requires specific bioinformatics tools.

Here, we present a pipeline for processing raw MPRA data obtained by NGS for reporter construct libraries with a priori unknown sequences of BCs and ROIs. The pipeline robustly identifies unambiguous (so-called genuine) BCs and ROIs associated with them, calculates the normalized expression level for each BC and the averaged values for each ROI, and provides a graphical visualization of the processed data.
Structured arrays of liquid microdroplets levitating over a heated liquid film have been observed in experimental works, but the nature of this phenomena is not completely understood. The aim of this work is to measure the distance between drops and the size of monolayer depending on the main parameters. We found that the average distance between drops increases with time from 40 to 100 μm. And the size of monolayer increases with time from approximately 1 mm to 5 mm. The distance between drops increases when approaching the boundary of monolayer.
We present the design, implementation and validation of the software procedure used to perform geometric calibration of the electromagnetic calorimeter with respect to the tracking system of the SND detector (BINP, Novosibirsk).

This procedure is based on the mathematical model describing the relative calorimeter position by means of a set of parameters. The parameter values are determined by minimizing χ2 function using the difference between directions reconstructed in these two subdetectors for e+e− → e+e− data events.
New experimental results on magnetic compression and transport of low-temperature arc plasma are presented. In the experiments, the transport efficiency of the plasma stream in a multiple-mirror configuration was compared with the same in a uniform solenoidal field. The magnetic compression ratio of the plasma stream varied from 5 to 60 at the initial diameter of 5 cm. As the result, the plasma with (1 – 4)×1020 m-3 density at the axis was obtained at ∼3 m distance from the plasma source. Theory predicted that a multiple-mirror magnetic field should not significantly decelerate and weaken collisional plasma. The experiment confirmed this prediction. In the presented work, we simulated the baseline scenario of GOL-NB filling by cold start plasma.
The house musk shrew, Suncus murinus, is polymorphic for five Robertsonian translocations (Rb8.17, 9.13, 10.12, 11.16, 14.15). Fluorescence in situ hybridisation with a biotin-labelled oligonucleotide, (TTAGGG)7, was performed to localise the telomeric DNA sequences at Rb chromosomes of heterozygous shrews. Hybridisation signals were observed at both ends of all chromosomes, but not at the pericentromeric areas of any of the Robertsonian metacentrics. Our results indicate a complete loss of the telomeric sequences at the fusion points of the Rb metacentrics in S. murinus.
Алелі ге наPpd-D1 у зраз ках ко лекції Aegilops tauschii і м'я кої пше ниці Г. О. Че бо тар 1 , С. В. Че бо тар 1, 2 , Д. О. Ба бен ко 2 , І. І. Моц ний 3 , А. Б. Щер бань 4 , Ю. М. Cи во лап 1 1 Півден ний біот ех но логічний центр в рос лин ництві Овідіопольська дорога, 3, Оде са, Украї на
Estimation of functional connectivity in gene sets derived from genome-wide or other biological experiments is one of the essential tasks of bioinformatics. A promising approach for solving this problem is to compare gene networks built using experimental gene sets with random networks. One of the resources that make such an analysis possible is CrossTalkZ, which uses the FunCoup database. However, existing methods, including CrossTalkZ, do not take into account individual types of interactions, such as protein/protein interactions, expression regulation, transport regulation, catalytic reactions, etc., but rather work with generalized types characterizing the existence of any connection between network members.

We developed the online tool FunGeneNet, which utilizes the ANDSystem and STRING to reconstruct gene networks using experimental gene sets and to estimate their difference from random networks. To compare the reconstructed networks with random ones, the node permutation algorithm implemented in CrossTalkZ was taken as a basis. To study the FunGeneNet applicability, the functional connectivity analysis of networks constructed for gene sets involved in the Gene Ontology biological processes was conducted.

We showed that the method sensitivity exceeds 0.8 at a specificity of 0.95. We found that the significance level of the difference between gene networks of biological processes and random networks is determined by the type of connections considered between objects. At the same time, the highest reliability is achieved for the generalized form of connections that takes into account all the individual types of connections. By taking examples of the thyroid cancer networks and the apoptosis network, it is demonstrated that key participants in these processes are involved in the interactions of those types by which these networks differ from random ones. FunGeneNet is a web tool aimed at proving the functionality of networks in a wide range of sizes of experimental gene sets, both for different global networks and for different types of interactions. Using examples of thyroid cancer and apoptosis networks, we have shown that the links over-represented in the analyzed network in comparison with the random ones make possible a biological interpretation of the original gene/protein sets.
The paper is devoted to the topical problem of determining the sources of methane from observational data.

An algorithm based on the statistical optimization method used to estimate a time constant parameter is considered. To implement the algorithm, a variant of ensemble smoothing is used, which is an optimal estimate of the desired parameter based on observational data and forecast for a given time interval. This paper presents the implementation of the algorithm for real observational and forecast data, the results of a three-dimensional transport and diffusion model are taken as a mathematical model, and satellite measurement data are used as observational data. The paper contains a mathematical formulation of the problem, a scheme for its numerical implementation.

Methane fluxes are estimated in subdomains of the Earth’s surface for specified time intervals. The results of numerical experiments with model and real data are presented.
Free-radical substitution of the chlorine atoms in the fluoroboron-capped iron(II) dichloroclathrochelate with tetrahydrofuran radical derivatives was studied.The reaction proceeds with high regioselectivity and yields the monofunctionalized macrobicyclic product.The molecular structure of this complex was determined both in solution and in the solid state using multinuclear NMR and the single-crystal X-ray diffraction.
Correction for ‘Na4Ca(CO3)3: a novel carbonate analog of borate optical materials’ by Sergey V. Rashchenko et al., CrystEngComm, 2018, 20, 5228–5232.
Radiation-chemical synthesis of silver nanoparticles was studied.

The silver nanoparticles in arabinogalactan (AG) water solution are stabilized in conglomerates, it is fixed by rise of additional bands in the optical absorption spectra. Pre-radiation treatment of AG causes crosslinking and oxidation.

Pretreated AG solution increases the stability of conglomerates containing silver nanoparticles in case of dilution.
Broadband terahertz polarizers based on one-dimensional metal grids on carrying thin-film polymeric substrates of different thicknesses were manufactured by photolithography and investigated with terahertz time-domain spectroscopy (THz-TDS). Polarizers patterned on 4 µm polypropylene (PP) showed the best maximum power transmission >98%. Two 4-μm-PP polarizers assembled as a tandem structure exhibited minimum power transmission 10000:1 which exceeded the dynamic range of THz-TDS. The developed polarizers were used to investigate polarization characteristics of the THz-TDS system.
Electron spin-lattice relaxation of two trityl radicals, d24-OX063 and Finland trityl, were studied under conditions relevant to their use in dissolution dynamic nuclear polarization (DNP).

The dependence of relaxation kinetics on temperature up to 100 K and on concentration up to 60 mM was obtained at X- and W-bands (0.35 and 3.5 Tesla, respectively). The relaxation is quite similar at both bands and for both trityl radicals.

At concentrations typical for DNP, relaxation is mediated by excitation transfer and spin-diffusion to fast-relaxing centers identified as triads of trityl radicals that spontaneously form in the frozen samples. These centers relax by an Orbach-Aminov mechanism and determine the relaxation, saturation and electron spin dynamics during DNP.
We study parametric instability of compact axion dark matter structures decaying to radiophotons. Corresponding objects - Bose (axion) stars, their clusters, and clouds of diffuse axions - form abundantly in the postinflationary Peccei-Quinn scenario. We develop general description of parametric resonance incorporating finite-volume effects, backreaction, axion velocities and their (in)coherence. With additional coarse-graining, our formalism reproduces kinetic equation for virialized axions interacting with photons. We derive conditions for the parametric instability in each of the above objects, as well as in collapsing axion stars, evaluate photon resonance modes and their growth exponents. As a by-product, we calculate stimulated emission of Bose stars and diffuse axions, arguing that the former can give larger contribution into the radiobackground. In the case of QCD axions, the Bose stars glow and collapsing stars radioburst if the axion-photon coupling exceeds the original KSVZ value by two orders of magnitude. The latter constraint is alleviated for several nearby axion stars in resonance and absent for axion-like particles. Our results show that the parametric effect may reveal itself in observations, from FRB to excess radiobackground.
Hybrid sterility is an important step in the speciation process. Hybrids between dwarf hamsters Phodopus sungorus and P. campbelli provide a good model for studies in cytological and genetic mechanisms of hybrid sterility. Previous studies in hybrids detected multiple abnormalities of spermatogenesis and a high frequency of dissociation between the X and Y chromosomes at the meiotic prophase.

In this study, we found that the autosomes of the hybrid males and females underwent paring and recombination as normally as their parental forms did. The male hybrids showed a significantly higher frequency of asynapsis and recombination failure between the heterochromatic arms of the X and Y chromosomes than the males of the parental species. Female hybrids as well as the females of the parental species demonstrated a high incidence of centromere misalignment at the XX bivalent and partial asynapsis of the ends of its heterochromatic arms. In all three karyotypes, recombination was completely suppressed in the heterochromatic arm of the X chromosome, where the pseudoautosomal region is located.

We propose that this recombination pattern speeds up divergence of the X- and Y-linked pseudoautosomal regions between the parental species and results in their incompatibility in the male hybrids.
This paper presents preliminary results of the effectiveness analysis of an air quality forecasting system for the city of Novosibirsk with replenishment of the missing information on emission sources by solving an inverse problem with urban monitoring network data. In solving the inverse problem, a priori information about the location and mode of the sources is used. To simulate concentration distributions, the WRF-Chem model is used, and a simplified model of chemical transport is applied to solving the inverse problem. These models are offline coupled in a hybrid forecast system in order to improve the initial information about the spatial distribution of emission intensity and air quality forecast, respectively. The results of numerical experiments and their analysis are presented. The influence of an urban parameterization on the results of the forecast is shown.
We have synthesized the novel complexes cis-[Ru(NO)Py2Cl2(OH)] and cis-[Ru(NO)Py2Cl2(H2O)]Cl that can be potential photoactive and bioactive materials.
The causative agent of opisthorchiasis, the liver fluke Opisthorchis felineus (Rivolta, 1884) is one of the helminths of humans and animals in Russia. Together with closely related species of trematodes O. viverrini (Poirier, 1886) and Clonorchis sinensis (Loos, 1907), O. felineus is a part of a triad of epidemiologically important trematodes in the family Opisthorchiidae. Adult O. felineus worms infest the hepatobiliary system of warm-blooded animals and might provoke the development of severe pathologies, including malignancy of bile duct epithelium. The high medical importance of O. felineus attracts the attention of researchers. This review briefly summarizes the data about O. felineus genomics and proteomics. The review provides a comparative analysis of the number of genes and sizes of nuclear genomes of a number of flatworms, the distribution of intron lengths, as well as results of synteny between the O. felineus , O. viverrini and C. sinensis genomes. Special attention is paid to a particular form of RNA processing known as trans-splicing, widely presented in the opisthorchiid genomes. We also provide the results of a comparative analysis of the xenobiotic metabolizing system between parasitic and free-living flatworms. Moreover, data on parasitic granulins, which are potential promoters of cholangiocyte neoplasia, are also presented. Data on the O. felineus genomics and proteomics provide first insights into the structural and functional organization of the genome of this parasitic flatworm with a complex life cycle as well as provide a significant contribution to our understanding of "host-parasite" interaction and evolution of this group of parasitic flatworms.
Forensic authorship analysis is a frequently used technique to identify the real author of an arguable document. Often enough, under study are interrogation minutes. This kind of text is difficult for examination because of its stylistic and genre characteristics: formal phrases and structure as well as different author and compiler of the document. The above features restrict the use of some levels of language analysis. This issue, however, is poorly covered in specialist literature, with only a few articles related to it. First we look at conventional techniques of authorship expertise and discuss their limitations. Special attention is given to the analysis of the interrogation minutes genre characteristics and their influence on the whole set of identifiers.

The current paper describes the main discursive features of interrogation minutes used in authorship expertise. Our technique is based on the methods of The Federal Ministry of the Interior, but it also takes into account genre characteristics of the interrogation minutes. A new classification of identifiers has been developed. Additional features are offered to improve the attribution accuracy. These are clarifications, which are classified according to the semantic type of the object. In the article clarifications are divided into six types and a few subtypes and are also divided into low and high informative ones. The analysis of clarification is illustrated with the example of three different interrogation minutes.

The analysis of several conventional interrogation minutes techniques singled out two central tendencies in the authorship attribution: an identification features selection with new identifiers being added. The concluding part of the article is concerned with the techniques of the interrogation minutes used in authorship expertise description, materials requirements and the steps of the analysis.
The history of the Central Asian Orogenic Belt (CAOB) was marked by several major events of magmatism which produced large volumes of volcanic and intrusive (mafic-ultramafic and granitic) rocks within a relatively short time span (30–40 Ma) over a vast area. The magmatic activity postdated the orogenic stages of accretionary-collisional belts in Central Asia and likely resulted from the impact of mantle plumes that formed Large Igneous Provinces (LIPs). The formation of the Tarim–South Mongolia LIP at 300–270 Ma is the best known among the major Permian events of basaltic and granitic magmatism. Early Permian igneous rocks (volcanic, subvolcanic and intrusive suites that vary from ultramafic to felsic compositions) of the same age range (300 to 270 Ma) have been recently found also in Eastern Kazakhstan, within the late Paleozoic Altai collisional system. The compositions and ages of the rocks suggest that the Eastern Kazakhstan magmatism was the northward expansion of the Tarim LIP. The spread of the Tarim LIP was apparently facilitated by lithospheric extension after the Siberia-Kazakhstan collision. The extension led to rheological weakening of the lithosphere whereby deep mantle melts could penetrate to shallower depths. The early Permian history of Eastern Kazakhstan was controlled by the interplay of plate tectonic and plume processes: plate-tectonic accretion and collision formed the structural framework, and the Tarim mantle plume was a heat source maintaining voluminous magma generation.
The validity of the use of a subjective-objective indicator of health as a significant indicator of its association with the prevalence of coronary artery disease is shown in both domestic and foreign scientific literature. At the same time, the subjective-objective indicator of health, and first of all, the attitude to one's health, is recognized as an indicator of a lifestyle that establishes the interpretation of conventional risk factors for coronary artery disease and interest in preventive measures at the population level.

Purpose: to determine the attitude towards one's health in the age range and in groups with and without coronary artery disease in an open population of a medium-urbanized city of Western Siberia in terms of gender. Material and methods. Cross–sectional studies were carried out in the framework of two cardiac screenings on unorganized subpopulations of men and women 25–64 years old in Tyumen (with responses 85.0 and 70.3 %, respectively). The isolation of various forms of coronary heart disease was carried out on the basis of standard epidemiological methods, the comparison group was defined as the absence of coronary heart disease. The attitude of the population to their health was established using a rigidly standardized WHO questionnaire MONICApsychosocial «Knowledge and attitude to the health».

Results. In the open population of a moderately urbanized city of Western Siberia, with a predominance among women, a low level of attitude towards their health was established, the prevalence of low responsibility for their health in the gender aspect took place in the third, fourth and sixth decades of life. Negative attitudes towards their health among persons of both sexes were determined mainly in groups with CHD; in women, the tendency towards a decrease in responsibility for their health was more pronounced than in men. Conclusion. When planning a preventive program for the population of a medium-urbanized city of Western Siberia, taking into account the priority impact on the subjective-objective indicator of population health, the implementation of the population strategy will probably not be enough. Taking into consideration the obtained regularities of the growth of negative attitudes towards their health among women, especially in groups with the presence of coronary heart disease, along with the population strategy, it would be optimal to use a high-risk strategy.
Дифференциальные соотношения между значениями ЭЛП различных порядков, действующих на стационарные или динамические распределения источников f , послужили основой для установления дифференциальных связей между тензорными полями угловых моментов различных порядков k и валентностей p.

Рассматриваются операторы угловых моментов, отображающих значения обобщённых экспоненциальных лучевых преобразований (ЭЛП) в множество симметричных p-тензорных полей.

Отмечены частные случаи, позволяющие получить некоторые ранее известные результаты.Связи ЭЛП порядка k с задачами томографии и