Hengzongshu/ArticleAgent huggingface dataset:
Background: Recent studies have demonstrated that the assessment of postural performance may be a potentially reliable and objective marker of the psychomotor retardation (PMR) in the major depressive disorder (MDD). One of the important facets of MDD-related PMR is reflected in disrupted central mechanisms of psychomotor control, heavily influenced by compelling maladaptive depressive rumination.

In view of this, we designed a research paradigm that included sequential execution of simple single posture task followed by more challenging divided attention posture tasks, involving concurring motor and ideomotor workloads. Another difficulty dimension assumed executing of all the tasks with eyes open (easy) and closed (difficult) conditions. We aimed at investigating the interplay between the severity of MDD, depressive rumination, and efficiency of postural performance. Method: Compared with 24 age- and body mass index-matched healthy controls (HC), 26 patients with MDD sequentially executed three experimental tasks: (1) single posture task of maintaining a quiet stance (ST), (2) actual posture-motor dual-task (AMT); (3) mental/ imaginary posture-motor dual-task (MMT). All the tasks were performed in the eyes open (EO) and the eyes closed (EC) conditions. The primary dependent variable was the amount of kinetic energy (E) expended for center of pressure deviations (CoPD), whereas the absolute divided attention cost index showed energy cost to the dual-tasking vs. the single posture task according to the formula: ΔE = (EDual-task - ESingle-task).

Result: The signs of PMR in the MDD group were objectively indexed by deficient posture control in the eyes closed condition along with overall slowness of fine motor and ideomotor activity. Another important and probably more challenging feature of the findings was that the posture deficit manifested in the ST condition was substantially and significantly attenuated in the MMT and AMT performance dual-tasking activity. A multiple linear regression analysis evidenced further that the dual-tasking energy cost (i.e., ΔE) significantly predicted clinical scores of severity of MDD and depressive rumination. Conclusion: The findings allow to suggest that execution of concurrent actual or imaginary fine motor task with closed visual input deallocates attentional resources from compelling maladaptive depressive rumination thereby attenuating severity of absolute dual-tasking energy costs for
The probe sampling of gases is extensively used in studies of high-temperature oxidation processes. This technique allows one to obtain directly information on the chemical composition of the reaction volume, however, inevitably introduces thermal and gas-dynamic perturbations into the system.

In order to further numerically simulate the sampling process, the temperature profiles of the quartz probe surface in the CH4/O2/Ar flames were measured in a pressure range of 1-5 atm. The measurements were carried out by thermographic method using an IR camera, which was calibrated by direct thermocouple measurements taking into account flame shielding.

The data obtained will be used as boundary conditions for computer simulation of the sampling processes, which will significantly improve the accuracy of the sampling probe methods.
Urinary albumin excretion (UAE) is widely used in clinical practice as indicator of diabetic kidney disease. According to the classical concept of the natural course of diabetic nephropathy, an increase in UAE usually precedes a decline in renal function. Meanwhile, a growing body of evidences indicates a high prevalence of normoalbuminuric chronic kidney disease (NA-CKD) in diabetic subjects, especially among patients with type 2 diabetes. An increase in NA-CKD prevalence can be results of improved glucose, blood pressure, and lipid control, widespread use of renin-angiotensin system blockers, and smoking cessation. It was shown that NA-CKD is more prevalent among women and is associated with arterial hypertension and coronary artery disease. The renal structure in subjects with NA-CKD is more heterogeneous when compared to patients with increased albuminuria, wherein interstitial changes and arteriolosclerosis could be the principal morphological findings, while signs of glomerulopathy may be absent. The prognostic value of NA-CKD needs to be clarified. It was shown that NA-CKD increases the risk of myocardial infarction, stroke and cardiovascular death in patients with diabetes. The search for alternative diagnostic markers for detecting of diabetic kidney disease in the absence of albuminuria, is of practical importance. The evaluations of the markers of tubular damage and interstitial fibrosis, as well as proteomic approaches, are considered as perspective diagnostic and prognostic options in NA-CKD. The study of pathogenesis, pathology, clinical course of NA-CKD in diabetic patients, as well as the development of more specific diagnostic and treatment options is a challenge for future research.
Melanins are a class of darkly pigmented biopolymers which are widely distributed among living organisms. The molecular and cellular mechanisms adopted by bacteria, fungi and animals to synthesize melanin, have been well described, but less is known regarding their production in plants. Here, a pair of barley near isogenic lines, bred to differ with respect to the pigmentation of the spike, was compared in order to understand the tissue and cellular location of melanin deposition. The melanic nature of the pigments purified from black spikes was confirmed by a series of solubility tests and Fourier transform infrared spectroscopy. An analysis of grains harvested at various stages of their development revealed that intracellular pigmented structures first appeared in the pericarp and the husk of black spike plants at early dough stage. The co-localization of these structures with red autofluorescence suggested that they form in chloroplast-derived plastids, here designated “melanoplasts”. Differences in dynamics of plastid internal structure during grain ripening were detected between the lines by transmission electron microscopy. Both lines accumulated plastoglobuli inside plastids, which persisted in black grain pericarp tissue up to the hard dough stage, while neither plastoglobuli nor any plastids were observed in grain of the control line at this stage. The role of plastoglobuli in melanin synthesis is discussed.
Purpose: To determine gender differences in trends of anxiety, depression and vital exhaustion levels in an open population aged 25-64 years over long-term period - 23 years in Russia / Siberia (Novosibirsk). Methods: Within the framework of the screening in 1994-95 under the WHO MONICA-psychosocial (MOPSY) program (n = 1527, 43% males, mean age 44.85 ± 0.4 years), in 2003-2005 under the international project HAPIEE (n=1650, 34,9% males, mean age 54,25±0,2 years), in 2013-2016 (n=975, 43,8% males, mean age 34,5±0,4 years) and 2016-2017 гг.( n=663, 41,3% years 51,95±0,32 years) within the framework of the budgetary theme No. АААА-А17-117112850280-2, random representative samples of men and women in one of districts in Novosibirsk were examined. Anxiety traits studied by means of the Spielberger test. Depression and vital exhaustion assessed by MOPSY questionnaires. Results: 2/3 of the female population aged 25-64 years had high level of anxiety traits in 1994. It was highest in the younger age groups. High anxiety was found in less than half of the surveyed men, increasing with age. The maximum values of anxiety were noted in 2003-2005 in both genders. The decrease in the incidence of high anxiety which began in 2013-2016, remained only in the female part of the population aged 35-64y but in men the prevalence of anxiety returned to the levels of 1994. Depression occurred in more than half of the female population in 1994. The overall prevalence among men was less than 30%. At the same time, the frequency of major D in women was 4-fold higher compared with men (p <0.001). Trends in prevalence in 2017 were mixed: a reduction in moderate levels and an increase in major depression in the youngest and oldest age groups. The prevalence of high vital exhaustion in 1994 was 14.6% and 31% in men and women 25-64 years, respectively (p <0.001). An increase in high exhaustion from younger to older age groups was noted in both sexes. The downward trend in exhaustion in 2017 persisted only among women. At that moment for the first time men began to report high exhaustion more often than women over 23-year of follow-up (16.9% and 15.6% for men and women 35-64y, respectively; n.s.). Conclusion: The prevalence of affective states is higher in females in general population. Long-term trends in decreasing the prevalence of anxiety, depression and vital exhaustion were not sustainable and returned to levels of 1994.
The aim of the research was to substantiate the need to take into account the factors that have a significant impact on the root rot infestation of spring wheat in the process of practical selection for resistance. The studies were carried out in 2019–2020 with the application of generally accepted and author’s methods. A systemic restructuring of work is needed to increase the efficiency of practical breeding for resistance to root rot. The damaging factor is cereal flies which “open the gates” to all soil phytopathogens after the tillering phase. It should be eliminated, and the ability of varieties to induce the suppression of rhizosphere soil to main phytopathogens should be taken into account. According to the results of two years of research, the smallest, within two biological thresholds of harmfulness (PV), the infestation by root rot at the germination phase was shown by the spring wheat varieties Novosibirskaia 15 and Manu. The development of root rot in comparison with the germination phase in 2019 increased by an average of 2.5, and in 2020 - by 5.6 times. There was an equalization of differences in damage between varieties during the growing season. In the germination phase, the coefficient of variation in the development of root rot by varieties was 24.1 ± 4.0, in the phase of maturity, it decreased 2.5 times to 9.4 ± 1.5. The main reason for smoothing out the differences between varieties in terms of root rot infestation was strong (up to 100%) damage by intra-stem pests. The economic threshold of harmfulness for damage to spring wheat stems by cereal flies was exceeded in 2019 up to 4.8 times, and in 2020 up to 7.8 times. The correlation coefficient between the development of root rot and damage by intra-stem pests in 2019 was R = 0.744 ± 0.185 (P <0.01), in 2020 R = 0.713 ± 0.092 (P <0.01). Analysis of variance showed that the power of the influence of the spatial arrangement of plants on their damage by cereal flies was 30.9% and was reliable at the 1% level, while the influence of the variety itself on this indicator was almost 2 times lower (16.8%) and was significant at the 5% level. The induction of soil suppression to phytopathogens by the rhizosphere of spring wheat varieties influenced their representation in the pathogenic complex of root rot. The correlation coefficient of induction by cultivars of suppressiveness to F. poae and its representation in pathogenic complexes was R = - 0.638 ± 0.318 and was significant at the 5% level. The correlation coefficient of induction of suppressiveness to F. oxysporum and its presence in pathogenic complexes of root rot of varieties was R = - 0.844 ± 0.203 (P <0.01).
Exosomes are important intercellular communication vehicles, secreted into body fluids by multiple cell types, including tumor cells. They contribute to the metastatic progression of tumor cells through paracrine signalling. It has been recently discovered that blood circulating exosomes contain distinguishable fractions of free and cell-surface-associated vesicles.

We evaluated the influence of protein cargoes from exosomes from plasma, and exosomes from the total blood of healthy females (HFs) and breast cancer patients (BCPs), on cell motility. We conducted a mass spectrometric analysis of exosomal contents isolated from samples using ultrafiltration and ultracentrifugation approaches and verified their nature using transmission electron microscopy, nanoparticle tracking analysis and flow cytometry.

We observed that malignant neoplasm-associated proteins in exosomes from BCP total blood were detected more often than in plasma (66% vs. 59%). FunRich analysis to assess Gene Ontology (GO) enrichment revealed that proteins with catalytic activities, transporter functions and protein metabolism activities were increased in exosomes from BCP blood. Finally, GO analysis revealed that proteomic profiles of exosomes from HF total blood were enriched with proteins inhibiting cell migration and invasion, which explains the low stimulating activity of exosomes from HF total blood on SKBR-3 cancer cell migration velocity. This allows exosomes to act as intermediaries providing intercellular communications through horizontal transfer of RNA and functionally active proteins, potentially affecting the development of both primary neoplasms and distant metastases.
The aim of the study is to investigate the ability of ISIAH rats with stress-sensitive arterial hypertension to cope with the salt loading.

Hypertensive ISIAH and normotensive WAG rats were kept in metabolic cages for 7 days on three drinking regimes: group 1 -no salt loading (tap water), group 2 -saline solution (0.87% NaCl), and group 3 -1,5% NaCl solution.After 7 days, no significant changes in the blood pressure in either WAG or ISIAH rats was observed.No differences between hypertensive and normotensive rats were found in the ability to excrete NaCl with urine.Glomerular filtration rates in ISIAH rats receiving both isotonic (saline) and hypertonic salt solutions were significantly higher than in the corresponding groups of WAG rats.In ISIAH, but not in WAG rats, receiving a hypertonic salt solution was accompanied by an increase in the concentrations of norepinephrine and epinephrine in urine.No significant changes in the function of renin-aldosterone system were observed.

These and previously obtained results lead to the conclusion that not renal mechanisms but rather sympathetic nervous activity underlies the early development of arterial hypertension in stress-sensitive ISIAH rats, which are well tolerated to the salt loadings at this period of ascending pathology.
Being one of the most dynamic entities in the human body, glycosylation of proteins fine-tunes the activity of the organismal machinery, including the immune system, and mediates the interaction with the human microbial consortium, typically represented by the gut microbiome.

Using data from 194 healthy individuals, we conducted an associational study to uncover potential relations between the gut microbiome and the blood plasma N-glycome, including N-glycome of immunoglobulin G.

While lacking strong linkages on the multivariate level, we were able to identify associations between alpha and beta microbiome diversity and the blood plasma N-glycome profile. Moreover, for two bacterial genera, namely, Bilophila and Clostridium innocuum, significant associations with specific glycans were also shown. The study's results suggest a non-trivial, possibly weak link between the total plasma N-glycome and the gut microbiome, predominantly involving glycans related to the immune system proteins, including immunoglobulin G. Further studies of glycans linked to microbiome-related proteins in well-selected patient groups are required to conclusively establish specific associations.
LVSD, even when asymptomatic, confers increased morbidity and mortality. We recently derived AI-ECG to detect LVSD using ECGs based on a large sample of patients treated at the Mayo Clinic.

We performed an external validation study with subjects from the Know Your Heart Study, a cross-sectional study of adults aged 35–69 years residing in two cities in Russia, who had undergone both ECG and transthoracic echocardiography. LVSD was defined as left ventricular ejection fraction ≤ 35%. We assessed the performance of the AI-ECG to identify LVSD in this distinct patient population.

The AI-ECG detected LVSD with robust test performance in a population that was very different from that used to develop the algorithm. Population-specific cut-offs may be necessary for clinical implementation. Differences in population characteristics, ECG and echocardiographic data quality may affect test performance.
We introduce a number of random matrix models describing the Google matrix G of directed networks. The properties of their spectra and eigenstates are analyzed by numerical matrix diagonalization. We show that for certain models it is possible to have an algebraic decay of PageRank vector with the exponent similar to real directed networks. At the same time the spectrum has no spectral gap and a broad distribution of eigenvalues in the complex plain. The eigenstates of G are characterized by the Anderson transition from localized to delocalized states and a mobility edge curve in the complex plane of eigenvalues.
Calculations describing these excitations by $t$-channel meson exchange are at variance with experimental differential cross sections and underpredict substantially the measured total cross section.

Exclusive measurements of the quasi-free $pp \to pp\pi^+\pi^-$ reaction have been performed by means of $pd$ collisions at $T_p$ = 1.2 GeV using the WASA detector setup at COSY. Total and differential cross sections have been obtained covering the energy region $T_p = 1.08 - 1.36$ GeV ($\sqrt s$ = 2.35 - 2.46 GeV), which includes the regions of $N^*(1440)$ and $\Delta(1232)\Delta(1232)$ resonance excitations.

An isotensor $\Delta N$ dibaryon resonance with $I(J^P) = 2(1^+)$ produced associatedly with a pion is able to overcome these deficiencies. Such a dibaryon was predicted by Dyson and Xuong and more recently calculated by Gal and Garcilazo.
Depolymerisation of the MT cytoskeleton during late prophase makes it impossible to follow the cytoskeleton cycle in centrosomeless plant meiocytes.

This paper describes rearrangements of the MT cytoskeleton during plant meiotic spindle formation in normally dividing pollen mother cells in various higher plant species and forms in which the cytoskeleton does not depolymerise at prophase. In such variants of the wild-type, cytoskeleton rearrangements can be observed at late prophase/early prometaphase. Radial MT bundles coalesce in the meridian plane, reorientate tangentially, curve and give rise to a developed ring-shaped perinuclear cytoskeleton system at the meridian. During nuclear envelope breakdown this ring disintegrates and splits into a set of free MT bundles. Three sub-stages of prometaphase are indicated: early prometaphase (disintegration of perinuclear ring and invasion of MTs into the former nuclear area), middle prometaphase or chaotic stage (formation of bipolar spindle fibres), and late prometaphase (formation of bipolar spindle).

Analysis of a range of abnormal phenotypes (disintegrated, multiple, polyarchal, chaotic spindles) reveals two previously unknown processes during late prometaphase: axial orientation and consolidation of the spindle fibres.
Sporadic Alzheimer’s disease (AD) is a severe disorder of unknown etiology with no definite time frame of onset. Recent studies suggest that middle age is a critical period for the relevant pathological processes of AD. Nonetheless, sufficient data have accumulated supporting the hypothesis of “neurodevelopmental origin of neurodegenerative disorders”: prerequisites for neurodegeneration may occur during early brain development.

Therefore, we investigated the development of the most AD-affected brain structures (hippocampus and prefrontal cortex) using an immunohistochemical approach in senescence-accelerated OXYS rats, which are considered a suitable model of the most common—sporadic—type of AD. We noticed an additional peak of neurogenesis, which coincides in time with the peak of apoptosis in the hippocampus of OXYS rats on postnatal day three. Besides, we showed signs of delayed migration of neurons to the prefrontal cortex as well as disturbances in astrocytic and microglial support of the hippocampus and prefrontal cortex during the first postnatal week.

Altogether, our results point to dysmaturation during early development of the brain—especially insufficient glial support—as a possible “first hit” leading to neurodegenerative processes and AD pathology manifestation later in life.
Free-radical substitution of the chlorine atoms in the fluoroboron-capped iron(II) dichloroclathrochelate with tetrahydrofuran radical derivatives was studied.

The reaction proceeds with high regioselectivity and yields the monofunctionalized macrobicyclic product.

The molecular structure of this complex was determined both in solution and in the solid state using multinuclear NMR and the single-crystal X-ray diffraction.
A computational and experimental study of the flow regimes of a mixture of castor and paraffin oils in a T‐type microchannel with 200 × 400 µm cross section was carried out. The ranges of parallel, slug, droplet, and rivulet flow regimes of the tested mixture were defined. According to the experimental results, a flow regime map was constructed for this mixture depending on the Weber number multiplied by the Ohnesorge number. A correlation of the length of paraffin oil slugs to the fluid flow ratio was established. The experimental data were compared with results of numerical simulation. A good agreement between calculation and experimental data was achieved in terms of reproduction of flow regimes, phase boundaries, and slug length.
Exosomes are 40–100 nm nanovesicles participating in intercellular communication and transferring various bioactive proteins, mRNAs, miRNAs, and lipids. During pregnancy, the placenta releases exosomes into the maternal circulation. Placental exosomes are detected in the maternal blood even in the first trimester of pregnancy and their numbers increase significantly by the end of pregnancy. Exosomes are necessary for the normal functioning of the placenta and fetal development. Effects of exosomes on target cells depend not only on their concentration but also on their intrinsic components. The biochemical composition of the placental exosomes may cause various complications of pregnancy. Some studies relate the changes in the composition of nanovesicles to placental dysfunction. Isolation of placental exosomes from the blood of pregnant women and the study of protein, lipid, and nucleic composition can lead to the development of methods for early diagnosis of pregnancy pathologies. This review describes the biogenesis of exosomes, methods of their isolation, analyzes their biochemical composition, and considers the prospects for using exosomes to diagnose pregnancy pathologies.