Hengzongshu/ArticleAgent huggingface dataset:
The article is devoted to the approach to geothermal zonality. The results of geothermal studies of oil and gas bearing deposits in the northern territory of Novosibirsk region and adjacent areas are presented. Geothermal zoning was carried out, and the major geothermal anomalies were determined. The features of the geothermal zonality of Mesozoic sediments in the region under investigation are first of all defined by the material composition of the Paleozoic basement, the structural arrangement, and the warming effect of ground water during its crossflows from deeper lying horizons. Formation temperatures get leveled within the boundaries of Aptian-Albian-Cenomanian sediments, and no anomalies are distinguished. The highest geothermal gradients sup to 3,6-4,6 °C/100 m and correspond to the edge zones of the development of intrusive formations of pre-Jurassic rocks. Lower gradients sup to 2,4-3,0 °C/100 m and characterize the regions, in which Paleozoic carbonate rocks occur.
Pulse wave velocity (PWV) has been recommended as an arterial damage assessment tool and a surrogate of arterial stiffness. However, the current technology does not allow to measure PWV both continuously and in real-time. We reported previously that peripherally measured ejection time (ET) overestimates ET measured centrally. This difference in ET is associated with the inherent vascular properties of the vessel. In the current study we examined ETs derived from plethysmography simultaneously at different peripheral locations and examined the influence of the underlying arterial properties on ET prolongation by changing the subject's position. We calculated the ET difference between two peripheral locations (ΔET) and its corresponding PWV for the same heartbeat. The ΔET increased with a corresponding decrease in PWV. The difference between ΔET in the supine and standing (which we call ET index) was higher in young subjects with low mean arterial pressure and low PWV. These results suggest that the difference in ET between two peripheral locations in the supine vs standing positions represents the underlying vascular properties. We propose ΔET in the supine position as a potential novel real-time continuous and non-invasive parameter of vascular properties, and the ET index as a potential non-invasive parameter of vascular reactivity.
A revision of mapping of main and alternative banding sequences in chromosome III (EF) has been made for 14 species of the Chironomusplumosus group. In total, new versions of mapping are presented for 18 banding sequences of arm E and 18 banding sequences of arm F. A new way of tracing the origins of banding sequences in chromosome III of the Ch.plumosus group in comparison with basic banding sequences of the genus Chironomus is suggested. The presented data indicate that h’pluE2 in arm E and p’borF2 in arm F are the closest to banding sequences of Ch.piger Strenzke, 1959 and thus should be considered the most ancient among banding sequences of chromosome III in the Ch.plumosus group. Phylogenetic relationships of banding sequences of chromosome III are discussed.
One of the challenges of modern biology and medicine is to visualize biomolecules in their natural environment, in real-time and in a non-invasive fashion, so as to gain insight into their physiological behavior and highlight alterations in pathological settings, which will enable to devise appropriate therapeutic strategies.

Genetically encoded fluorescent biosensors constitute a class of imaging agents that enable visualization of biological processes and events directly in situ, preserving the native biological context and providing detailed insight into their localization and dynamics in cells. Real-time monitoring of drug action in a specific cellular compartment, organ, or tissue type; the ability to screen at the single-cell resolution; and the elimination of false-positive results caused by low drug bioavailability that is not detected by in vitro testing methods are a few of the obvious benefits of using genetically encoded fluorescent biosensors in drug screening.

This review summarizes results of the studies that have been conducted in the last years toward the fabrication of genetically encoded fluorescent biosensors for biomedical applications with a comprehensive discussion on the challenges, future trends, and potential inputs needed for improving them.
This article examines Ivan Danilovich Khokhlovs mission to the Khanate of Bukhara in the early 17th century to gain a better understanding of the Russian envoys links with the Central Asian states. Working with the embassys report and related sources, the author looks at both the official and unofficial contacts of the missions members and pays particular attention to identifying with whom they dealt. Unusually, the diplomat limited himself to official contacts in building his information network. Together with his interpreter, Khohklov worked hard to set up a network of horizontal contacts to provide him with comprehensive and detailed intelligence. Endeavoring to improve relations, he described the ruling khans and those close to him, all the while making sure that he strictly adhered to Russian diplomatic protocol.
Results of experimental studies of features of the round hydrogen microjet combustion in a coaxial air jet are presented in this work. It is shown that the combustion scenario is connected with existence of the «bottleneck flame region». This fact correlates with the similar scenarios of the diffusion hydrogen microjet combustion at subsonic efflux velocity investigated by us earlier. It is revealed that the spherical shape of the “bottleneck flame region” is transformed to a cylindrical shape. It is found that the round hydrogen microjet combustion in a coaxial air jet at supersonic efflux velocity is accompanied by existence of supersonic cells both in a hydrogen microjet and in a wake of coaxial air jet. Round hydrogen microjet supersonic combustion in a coaxial air jet is connected with a flame separation from a nozzle exit.
ABSTRACT Introduction Lumbar intervertebral disc degeneration (DD) disease is one of the main risk factors for low back pain. The social and economic importance of low back pain is very high: back pain is among the leading causes of absenteeism and the cost of treating back pain exceeds the cost of treatment of many other serious diseases (cancer, in particular); however, therapy does not always provide the desired result. Despite the variability of biological studies of lumbar DD, it is still not fully understood, partially due to the fact that there are only few studies using systematic and integrative approaches. Hence, more integrative omics studies are needed to link all pieces of knowledge together, build a complete picture of biology of lumbar DD and obtain a deeper understanding of the processes underlying this pathology. Methods and analysis This disease-oriented biobank to study lumbar disc degeneration will be recruited from two clinical centers. A total of 1100 participants with available lumbar MRI will be enrolled during the three-year period. General information about a patient, medical history, lumbar MRI parameters and biological material (whole blood and plasma) will be collected in the centers at baseline. Then, from those patients, who will undergo a spine surgery during the treatment, disc tissue samples will be gained. Eventually, postoperative clinical data will be collected from operated patients during the follow-up. Ethics and dissemination The study will be performed according to the Helsinki Declaration. The study protocol was approved by the Local Ethical Committee of NRITO (№034/20 dated 02 Oct 2020) and by the Local Ethical Committee of Priorov CITO (№1/21 dated 25 Feb 2021). Preliminary and final results will be presented in peer-reviewed journals, at national and international congresses. Trial registration number NCT04600544 ARTICLE SUMMARY STRENGTHS AND LIMITATIONS OF THIS STUDY Two-center and multiple-discipline study: the study includes two centers (Moscow in the European and Novosibirsk in the Asian part of Russia), with research teams specializing in following fields: (1) clinical aspects of lumbar disc degeneration disease, (2) biology and genetics of pain, (3) generation of omics data and (4) multi-omics data analysis Collection of variable biological material: whole blood, plasma and, for part of the participants, intraoperative material of the lumbar disc (with different DD status) will be available for omics studies Objective diagnosis: lumbar DD status is confirmed by MRI, not self-reported diagnosis Sample size is limited compared to nationwide biobanks Patients with severe lumbar DD status will be more prevalent in the biobank than these with mild or no lumbar DD compared to the general population
Anoxybacillus flavithermus strain 25 was isolated from a sediment sample from the Garga hot spring in the Barguzin Valley, Baikal Region, Russian Federation (54°19'3.72″N, 110°59'38.4″E). The sequenced and annotated genome is 2,838,680 bp and encodes 3,009 genes.
Catalyst speciation during polymerization of 1-hexene in benzene or toluene solutions of the catalyst precursor SBIZr(μ-Me)2AlMe2+ B(C6F5)4− (SBI = rac-dimethylsilyl-bis(1-indenyl)) at 23 °C is studied by following the accompanying UV-vis-spectral changes. These indicate that the onset of polymerization catalysis is associated with the concurrent formation of two distinct zirconocene species. One of these is proposed to consist of SBIZr-σ-polyhexenyl cations arising from SBIZr-Me+ (formed from SBIZr(μ-Me)2AlMe2+ by release of AlMe3) by repeated olefin insertions, while the other one is proposed to consist of SBIZr-η3-allyl cations of composition SBIZr-η3-(1-R-C3H4)+ (R = n-propyl), formed by σ-bond metathesis between SBIZr-Me+ and 1-hexene under release of methane. At later reaction stages, all zirconocene-σ–polymeryl cations appear to decay to yet another SBIZr-allyl species, i.e., to cations of the type SBIZr-η3-(x-R-(3-x)-pol-C3H3)+ (pol = i-polyhexenyl, x = 1 or 2). Renewed addition of excess 1-hexene is proposed to convert these sterically encumbered Zr-allyl cations back to catalytically active SBIZr-σ–polymeryl cations within a few seconds, presumably by initial 1-hexene insertion into the η1- isomer, followed by repeated additional insertions, while the initially formed, less crowded allyl cations, SBIZr-η3-(1-R-C3H4)+ appear to remain unchanged. Implications of these results with regard to the kinetics of zirconocene-catalyzed olefin polymerization are discussed.
This work analyzes carrier transport, the relaxation of non-equilibrium charge, and the electronic structure of fluorinated graphene (FG) films with graphene quantum dots (GQDs).

The FG films with GQDs were fabricated by means of chemical functionalization in an aqueous solution of hydrofluoric acid. High fluctuations of potential relief inside the FG barriers have been detected in the range of up to 200 mV. A phenomenological expression that describes the dependence of the time of non-equilibrium charge emission from GQDs on quantum confinement levels and film thickness (potential barrier parameters between GQDs) is suggested.

An increase in the degree of functionalization leads to a decrease in GQD size, the removal of the GQD effect on carrier transport, and the relaxation of non-equilibrium charge. The study of the electronic properties of FG films with GQDs has revealed a unipolar resistive switching effect in the films with a relatively high degree of fluorination and a high current modulation in transistorlike structures with a lower degree of fluorination. 2D films with GQDs are believed to have considerable potential for various electronic applications (nonvolatile memory, 2D connections with optical control and logic elements).
In recent studies it was determined that certain allotriploids produced from crosses of sexual diploid Tripsacum dactyloides (2 n = 2 x = 36), section Tripsacum and apomictic tetraploid T. maizar (2 n = 4 x = 72), section Fasciculata reproduce sexually. Backcrossing progenies can be clearly classified to either sect. Tripsacum or sect. Fasciculata due to their distinctively different inflorescence phenotype. More importantly, the sectional delineation of the progeny shifted in the direction of the pollinator through genome substitution, which is not to be expected following just one generation of backcrossing in a normal breeding program. Our main objective was to investigate whether there were potential genetic differences between the genomes from sect. Tripsacum and sect. Fasciculata at the molecular level that correlate with such morphological shifts in these unusual sexual allotriploids. Cluster analysis based on randomly amplified polymorphic DNA (RAPD) profiles of these sexual allotriploids clearly indicated that substantial differences among many loci do exist between T. dactyloides and T. maizar . These results support the taxonomic classification of Tripsacum species into section Tripsacum and section Fasciculata . Copyright 2000 Annals of Botany Company
Background Alpha-synuclein (α-Syn) is a small neuronal protein that has been found to be expressed throughout the brain. It has been shown that α-Syn regulates the homeostasis of monoamine neurotransmitters and is involved in various degenerative and affective disorders. There is indication that α-Syn may regulate expression of the brain-derived neurotropic factor (BDNF) which plays an important role in the mood disorders.

Methodology/Principal Findings The study aimed to analyze the mRNA levels of Snca and Bdnf genes in the ventral tegmental area (VTA) and raphe nuclei of the midbrain in male mice that had each won or defeated 20 encounters (20-time winners and 20-time losers, respectively) in daily agonistic interactions. Groups of animals that had the same winning and losing track record followed by a no-fight period for 14 days (no-fighting winners and no-fighting losers) were also studied. Snca mRNA levels were increased in the raphe nuclei in the 20-time losers and in the VTA of the 20-time winners. After no-fight period Snca mRNA levels decreased in both groups. Snca mRNA levels were similar to the control level in the VTA of the 20-time losers and in the raphe nuclei of the 20-time winners. However Snca gene expression increased in these areas in the no-fighting winners and no-fighting losers in comparison with respective mRNA levels in animals before no-fight period. Bdnf mRNA levels increased in VTA of 20-time winners. Significant positive correlations were found between the mRNA levels of Snca and Bdnf genes in the raphe nuclei.

Conclusions/Significance Social experience affects Snca gene expression depending on brain areas and functional activity of monoaminergic systems in chronically victorious or defeated mice. These findings may be useful for understanding the mechanisms of forming different alpha-synucleinopathies.
Viruses have some characteristics in common with cell-based life. They can evolve and adapt to environmental conditions.

Directed evolution can be used by researchers to produce viral strains with desirable phenotypes. Through bioselection, improved strains of oncolytic viruses can be obtained that have better safety profiles, increased specificity for malignant cells, and more efficient spread among tumor cells. It is also possible to select strains capable of killing a broader spectrum of cancer cell variants, so as to achieve a higher frequency of therapeutic responses.

This review describes and analyses virus adaptation studies performed with members of four RNA virus families that are used for viral oncolysis: reoviruses, paramyxoviruses, enteroviruses, and rhabdoviruses.
We revisit, starting from the first principles, the calculation of scattering cross sections in theories with violation of the Lorentz invariance.

Our approach takes into account all information about the Lagrangian of the theory and is applicable to the case of Lorentz violation by higher-dimension operators. As an illustration, we consider spinor electrodynamics with Lorentz violating operators of dimension four and six that preserve $CPT$, parity, and rotational invariance in a preferred frame. We derive the Feynman rules and formulas for the sums over polarizations in this model and use them to compute the probabilities of several astrophysically relevant processes.

We calculate the rates of photon decay and vacuum Cherenkov radiation along with the cross sections of electron-positron pair production on background radiation and in the Coulomb field. The latter process is essential for detection of photon-induced air showers in the atmosphere.
A poor consumption of important nutrients triggered a public interest in functional foods that contain easy-to-digest proteins. The present research features fractionation, mechanical activation, and enzymatic hydrolysis of pea protein. According to modern chemical methods, the protein content in the original pea biomass was 24.3% and its molecular weight distribution (MWD) was 5–135 kDa. Fractionation, or protein displacement, resulted in four fractions of biopolymers with different chemical composition, i.e. a different content of protein and carbohydrate molecules. The paper introduces some data on the enzymatic transformations of the substrate. A set of experiments made it possible to define the optimal conditions for the mechanical activation of pea biomass with proteolytic enzymes. The enzymes were obtained from Protosubtilin G3x, a complex enzyme preparation. When the substrate and the enzymes were mechanically activated together, it produced mechanocomposite, an intermediate product with increased reactivity. It increased the specific surface area by 3.2 times and doubled the crystallinity of the substrate. As a result, the rate and yield of the subsequent enzymatic hydrolysis increased from 18% to 61%. The study determined the capacity of the substrate in relation to the enzyme preparation. Under optimal conditions, the pea hydrolysis destroyed protein molecules within two hours. After four hours of hydrolysis, no changes were detected. A polyacrylamide gel electrophoresis revealed non-hydrolysed protein molecules with MW ≈ 20 kDa. Presumably, they corresponded with legumin, which is resistant to neutral and alkaline proteases. The resulting hydrolysates were spray-dried to test their potential use as a food component. The product obtained by spray-drying had a monomodal distribution of particle sizes of spherical shape with adiameter of 5–20 μm.
Формы миграции химических элементов в подземных водах горизонта Ю 1 Верх-Тарского нефтяного месторождения (юг Западной Сибири)Новиков Д.А. 1, 2
A search for new heavy particles that decay into top-quark pairs is performed using data collected from proton-proton collisions at a centre-of-mass energy of 13 TeV by the ATLAS detector at the Large Hadron Collider. The integrated luminosity of the data sample is 36.1 fb -1 .

Events consistent with top-quark pair production are selected by requiring a single isolated charged lepton, missing transverse momentum and jet activity compatible with a hadronic top-quark decay. Jets identified as likely to contain b-hadrons are required to reduce the background from other Standard Model processes. The invariant mass spectrum of the candidate top-quark pairs is examined for local excesses above the background expectation.

No significant deviations from the Standard Model predictions are found. Exclusion limits are set on the production cross-section times branching ratio for hypothetical Z' bosons, Kaluza-Kein gluons and Kaluza-Klein gravitons that decay into top-quark pairs.
Aim. To study the associations of polymorphisms in F2 , F7 , and PAI1 genes with the presence of vulnerable plaque in coronary arteries (CA) and the blood concentration of proteins encoded by these genes.

Material and methods. The study included 101 men 40-70 years old with documented coronary atherosclerosis, who underwent coronary artery bypass grafting. According to the histological analysis of atherosclerotic plaques, men were divided into 2 groups: 40 men (39,6%) with stable plaque; 61 men (60,4%) with vulnerable plaques in CA. Genotyping of rs1799963 and rs6046 was performed by reverse transcription polymerase chain reaction, rs1799889 — by polymerase chain reaction. Statistical processing was performed using the SPSS 16.0 software package.

Results. In patients with stable plaques, allele A of rs6046 polymorphism in the F7 gene was observed in 2,9 times more often (95% confidence interval (CI), 1,20-7,20, p=0,021) than in men with vulnerable plaques. The odds ratio of the GA genotype carriage is 4,03 times higher among patients with stable plaques in CA compared with vulnerable plaques (95% CI, 1,49-10,93, p=0,006). The odds ratio of the 5G/4G genotype carriage among patients with stable plaques in CA is 2,47 times higher than in patients with vulnerable plaques (95% CI, 1,08-5,62, p=0,039). The 4G/4G genotype carriage is 5,85 times much more common in men with stable plaques (95% CI, 1,61-21,34, p=0,003). Conclusion. Polymorphism in the PAI1 (rs1799889) and F7 (rs6046) genes are associated with the presence of vulnerable plaques in CA in men with verified coronary atherosclerosis. There were no differences between the groups in the frequencies of genotypes and alleles of the rs1799963 polymorphism of the F2 gene. Also, no significant differences were found in the blood levels of PAI-1 and factor VII in groups with different genotypes.
В настоящей работе представлен новый метод решения уравнений движения заряженных частиц в электромагнитных полях и проведено его сравнение с различными известными модификациями метода Бориса. Созданные двумерный и трехмерный алгоритмы основаны на использовании точного решения дифференциального уравнения для скорости заряженной частицы на шаге по времени. Сравнительный анализ метода Бориса и его модификаций проводился как по точности методов, так и по времени их работы. Новая модификация метода Бориса позволяет точнее вычислять траекторию и скорость заряженной частицы без значительного увеличения сложности расчетов. Показано, что при выборе модификации метода Бориса для решения задачи в первую очередь следует обращать внимание на точность решения, так как более простая и быстрая схема может не дать выигрыша по времени. The article proposes a new method for solving the equations of motion of charged particles in electromagnetic fields and compares this method with various known modifications of the Boris method. The created two-dimensional and three-dimensional algorithms are based on the use of an exact solution of the differential equation for the velocity of a charged particle at a time step. A comparative analysis of the Boris method and its modifications was carried out both in terms of the accuracy of the methods and the time of their operation. A new modification of the Boris method allows more accurate calculations of the trajectory and velocity of a charged particle without a significant increase in the complexity of calculations. It is shown that, when choosing a modification of the Boris method to solve a problem, one should pay attention first of all to the accuracy of the solution, since a simpler and faster scheme may not give a gain in time.
A rapid growth in the number of genes with known sequences calls for developing automated tools for their classification and analysis. It became clear that nucleosome packaging of eukaryotic DNA is very important for gene functioning. Automated computer tools for characterization of nucleosome packaging density could be useful for studying of gene regulation and genome annotation.

A program for constructing nucleosome formation potential profiles of eukaryotic DNA sequences was developed. Nucleosome packaging density was analyzed for different functional types of human promoters.

It was found that in promoters of tissue-specific genes, the nucleosome formation potential was essentially higher than in genes expressed in many tissues, or housekeeping genes. Hence, capability of nucleosome positioning in the promoter region may serve as a factor regulating gene expression.