Hengzongshu/ArticleAgent huggingface dataset:
At the moment, one of the actual trends in medical diagnostics is a development of methods for practical applications such as point-of-care testing, POCT or research tools, for example, whole genome amplification, WGA. All the techniques are based on using of specific DNA polymerases having strand displacement activity, high synthetic processivity, fidelity and, most significantly, tolerance to contaminants, appearing from analysed biological samples or collected under purification procedures.

Here, we have designed a set of fusion enzymes based on catalytic domain of DNA polymerase I from Geobacillus sp. 777 with DNA-binding domain of DNA ligase Pyrococcus abyssi and Sto7d protein from Sulfolobus tokodaii, analogue of Sso7d. Designed chimeric DNA polymerases DBD-Gss, Sto-Gss and Gss-Sto exhibited the same level of thermal stability, thermal transferase activity and fidelity as native Gss; however, the processivity was increased up to 3-fold, leading to about 4-fold of DNA product in WGA which is much more exiting. The attachment of DNA-binding proteins enhanced the inhibitor tolerance of chimeric polymerases in loop-mediated isothermal amplification to several of the most common DNA sample contaminants-urea and whole blood, heparin, ethylenediaminetetraacetic acid, NaCl, ethanol.

Therefore, chimeric Bst-like Gss-polymerase will be promising tool for both WGA and POCT due to increased processivity and inhibitor tolerance.
Based on an inverse correlation between blood plasma DNase activity and the cfDNA concentration, blood DNases are supposed to regulate the cfDNA concentration. However, no correlation was found between the DNase activity in blood plasma and the csbDNA concentration, indicating that blood DNases are not involved in csbDNA dissociation from the cell surface.

A direct correlation between the concentration of cell-free and cell-surface-bound circulating DNA (cfDNA and csbDNA, respectively) was demonstrated.

The possibility of DNA redistribution between cfDNA and csbDNA indicates that the total pool of circulating DNA (cfDNA + csbDNA) should be used for a correct analysis of marker DNA concentrations and data standardization.
We demonstrate the applicability of our new method (the Dense Shell Method or DSM) for the determination of astronomical distances by calculating the distance to SN2009ip. The distance to this supernova has been accurately determined in the standard approach via the cosmic distance ladder and has been found to be 20.4 Mpc. Our direct method, assuming the most reasonable parameter values, gives a very close result, namely 20.1+/-0.8 (68% CL) Mpc to SN2009ip.
The role of pulse pressure 'widening' at older age, as a cardiovascular risk factor is still controversial. 24-hour ambulatory pulse pressure (PP) was previously expressed as a sum of two components: 'elastic' PP (elPP) and 'stiffening' PP (stPP) associated, respectively, with arterial stiffness at diastole and the change in stiffness during systole.

Longitudinal population-based cohort study of adults from Europe, Asia, and South America with baseline observations, including 24-hour ambulatory BP. Age classes were age < 50, 50–60, 60–70, and > 70 year. Co-primary endpoints were total mortality and composite CV events. Hazard ratios (HRs) per 1-SD increase in the PP variables were calculated from multivariable adjusted Cox regression models.

The age-related increase in PP variables is not reflected in a corresponding PP-associated increase in cardiovascular risk. Results support the view that normal ageing weakens the association of PP variables with CV risk.
Our aim was to study the association of hostility with the DRD4, DAT, MAOA genes in an open male population of 25–64 years old.

A representative sample of men aged 25–64 years (n = 657 men, average age 44.3 ± 0.4 years) was examined in 1994–1995 and 45–64 years old (n = 781 men, average age - 56.48 ± 0.2 years) in 2003–2005 using the methods proposed by the WHO international program “MONICA-psychosocial” and “HAPIEE”. All respondents completed the hostility questionnaire on their own. Genotyping of the DRD4, DAT and MAOA gene polymorphisms was carried out.

It was established that the level of hostility in the male population was 76.9% in the group of 25–64 years old and 60.3% in the group of 45–64 years old. Genotypes 4/6, 4/7 of the DRD4 gene are reliably associated with a high level of hostility; the genotype 4/4 of the DRD4 gene is associated with an average and lower level of hostility. There was no association of individual genotypes and VNTR alleles of DAT gene polymorphism with different levels of hostility. It was found that among individuals with low-active alleles of the MAOA-L gene (alleles 2 and 3), a high level of hostility was more common - 50.9%. The results of constructing a logistic regression model showed that the presence of low-active alleles (2; 3) of the MAOA gene increases the likelihood of hostility OR = 2.103 (95% CI 1.137–3.889, p = 0.018). Based on the received data we can assume that the long alleles of the DRD4 gene and the low-level allele of the MAOA-L gene are associated with hostility.
The paper aims to make а fair supplement to the concept of "metaphysics of relations" (bу М. Esfeld) with а coгrect coгresponding interpretation of the dispositional natшe of characteristics within Е. J. Lowe's ontology. А reasoning from science to philosophy leads М. Esfeld to the conclusion that "quantum entanglement understood in terms of non-separability of states speaks for the metaphysics of relations that denies the presence of intrinsic characteristics of the related systems".

As а heuristics for the intentional interpretation ofE. J. Lowe's dispositionalism we deal with the requirements of "time-relativeness" and "relevance to causality" from W. Malzkorn's list of criteria of satisfactory representation of dispositions.

The same naturalistic argument provides rationale for the Е. J. Lowe's claim that "the attribution of а characteristics to а given kind could bе metaphysically contingent" for "the laws of nature can bе exactly what they are revealed to us, only because there are no other substantial kinds that could instantiate objects".
Based on the use of panel data, the work establishes the relationship between the development of renewable energy sources and (1) the price of fossil energy, (2) the supply of oil and gas consumption through its own production, (3) the factor of the country's technological development and (4) the influence of non-competitive political institutions.

The period of high prices for fossil energy (1999-2014) became the catalyst for changes in the structure of the energy balance, especially in Europe, with an increase in the production of renewable energy. However, the fall in prices in 2014 - to the present has led to stagnation in the production of renewable energy with a rapid increase in the use of fossil fuels.

In the context of this work, the significance of the factors under study on the consumption of renewable energy sources is proved.
The long-term evolution of a circumstellar disk starting from its formation and ending in the T Tauri phase was simulated numerically with the purpose of studying the evolution of dust in the disk with distinct values of viscous α-parameter and dust fragmentation velocity v_frag. We solved numerical hydrodynamics equations in the thin-disk limit, which are modified to include a dust component consisting of two parts: sub-micron-sized dust and grown dust with a maximum radius a_r. The former is strictly coupled to the gas, while the latter interacts with the gas via friction. The conversion of small to grown dust, dust growth, and dust self-gravity are also considered. We found that the process of dust growth known for the older protoplanetary phase also holds for the embedded phase of disk evolution. The dust growth efficiency depends on the radial distance from the star - a_r is largest in the inner disk and gradually declines with radial distance. In the inner disk, a_r is limited by the dust fragmentation barrier. The process of small-to-grown dust conversion is very fast once the disk is formed. The total mass of grown dust in the disk (beyond 1 AU) reaches tens or even hundreds of Earth masses already in the embedded phase of star formation and even a greater amount of grown dust drifts in the inner, unresolved 1 AU of the disk. Dust does not usually grow to radii greater than a few cm. A notable exception are models with α<= 10^{-3}, in which case a zone with reduced mass transport develops in the inner disk and dust can grow to meter-sized boulders in the inner 10 AU. Grown dust drifts inward and accumulates in the inner disk regions. This effect is most pronounced in the α<= 10^{-3} models where several hundreds of Earth masses can be accumulated in a narrow region of several AU from the star by the end of embedded phase. (abridged).
SND detector operates at the VEPP-2000 collider (BINP, Novosibirsk). To improve events selection for physical analysis and facilitate online detector control we developed new data quality monitoring (DQM) system. The system includes online and reprocess control modules, automatic decision making scripts, interactive (web based) and program (python) access to various quality estimates. This access is implemented with node.js server with data in RDBMS MySQL. We describe here general system logics, its components and some implementation details.
Identified are the occurrence, serotypic and genotypic variations of Hepatitis B virus isolates (HBV) among the Novosibirsk region inhabitants (n=2000), native population of the Alarsk District of the Irkutsk Region (n=487) and Shuryshkarsk Township of the Yamalo-Nenets Autonomous District (n=657). Occurrence rate of hepatitis В surface antigen (HBsAg) among different groups of the Novosibirsk Region population varied within the limits of 3,6-35,0 %. It was 8,2 % in Alarsk District, and 3,2 % in Shuryshkarsk Township.

HBV isolates of D genotype (92-97 %) prevail among the population of Siberia; few are the cases of A (1,7 %) and C (1,2-8 %) genotypes.

The identified varying occurrence of HBV sub-genotypes and HBsAg subtypes in two aboriginal groups of Siberia (D3 sub-genotype and ayw2 subtype - in the Alarsk District, D2 and ayw3 - in Shuryshkarsk Township) suggests the existence of, at least, two isolated HBV virus populations, circulating among different groups of Siberia native population.
Transcripts of many enzymes involved in base excision repair (BER) undergo extensive alternative splicing, but functions of the corresponding alternative splice variants remain largely unexplored. In this review, we cover the studies describing the common alternatively spliced isoforms and disease-associated variants of DNA glycosylases, AP-endonuclease 1, and DNA polymerase beta. We also discuss the roles of alternative splicing in the regulation of their expression, catalytic activities, and intracellular transport.
To discover sources for novel anti-influenza drugs, we evaluated the antiviral potential of nine extracts from eight medicinal plants and one mushroom (Avena sativa L., Hordeum vulgare Linn. var. nudum Hook. f., Hippophae rhamnoides Linn., Lycium ruthenicum Murr., Nitraria tangutorum Bobr., Nitraria tangutorum Bobr. by-products, Potentilla anserina L., Cladina rangiferina (L.) Nyl., and Armillaria luteo-virens) from the Qinghai–Tibetan plateau against the influenza A/H3N2 virus.

Concentrations lower than 125 μg/mL of all extracts demonstrated no significant toxicity in MDCK cells. During screening, seven extracts (A. sativa, H. vulgare, H. rhamnoides, L. ruthenicum, N. tangutorum, C. rangiferina, and A. luteo-virens) exhibited antiviral activity, especially the water-soluble polysaccharide from the fruit body of the mushroom A. luteo-virens. These extracts significantly reduced the infectivity of the human influenza A/H3N2 virus in vitro when used at concentrations of 15.6–125 μg/mL. Two extracts (N. tangutorum by-products and P. anserina) had no A/H3N2 virus inhibitory activity. Notably, the extract obtained from the fruits of N. tangutorum and N. tangutorum by-products exhibited different anti-influenza effects.

The results suggest that extracts of A. sativa, H. vulgare, H. rhamnoides, L. ruthenicum, N. tangutorum, C. rangiferina, and A. luteo-virens contain substances with antiviral activity, and may be promising sources of new antiviral drugs.
Mariner -like elements (MLEs) are among the most widespread DNA transposable elements in eukaryotes. Insects were the first organisms in which MLEs were identified, however the diversity of MLEs in the insect order Orthoptera has not yet been addressed. In the present study, we explore the diversity of MLEs elements in 16 species of Orthoptera belonging to three infraorders, Acridoidea (Caelifera), Grylloidea (Ensifera), and Tettigoniidea (Ensifera) by combining data mined from computational analysis of sequenced degenerative PCR MLE amplicons and available Orthoptera genomic scaffolds. In total, 75 MLE lineages (Ortmar) were identified in all the studied genomes. Automatic phylogeny-based classification suggested that the current known variability of MLEs can be assigned to seven statistically well-supported phylogenetic clusters (I–VII), and the identified Orthoptera lineages were distributed among all of them. The majority of the lineages (36 out of 75) belong to cluster I; 20 belong to cluster VI; and seven, six, four, one and one lineages belong to clusters II, IV, VII, III, and V, respectively. Two of the clusters (II and IV) were composed of a single Orthoptera MLE lineage each (Ortmar37 and Ortmar45, respectively) which were distributed in the vast majority of the studied Orthoptera genomes. Finally, for 16 Orthoptera MLE lineages, horizontal transfer from the distantly related taxa belonging to other insect orders may have occurred. We believe that our study can serve as a basis for future researches on the diversity, distribution, and evolution of MLEs in species of other taxa that are still lacking the sequenced genomes.
ABSTRACT MicroRNAs (miRNAs) are key players in parasite-host communication and potential biomarkers for the detection of parasitic infections from host blood. Consequently, it is crucial to precisely know the miRNA complements of medically important agents such as the liver flukes of the Opisthorchiidae. Using publicly available and new datasets we curated and reannotated the surprisingly small and variable miRNA complements previously described for Opistorchis viverrini, O. felineus and Clonorchis sinesis. We find three highly similar miRNA complements with 53 identical and two miRNA genes with species specific sequences that signify a set of potential biomarkers and promising candidates for further investigations.
Hyperpolarization-enhanced magnetic resonance imaging can be used to study biomolecular processes in the body, but typically requires nuclei such as 13 C, 15 N, or 129 Xe due to their long spin‑polarization lifetimes and the absence of a proton‑background signal from water and fat in the images.

Here we present a novel type of 1 H imaging, in which hyperpolarized spin order is locked in a nonmagnetic long-lived correlated (singlet) state, and is only liberated for imaging by a specific biochemical reaction. In this work we produce hyperpolarized fumarate via chemical reaction of a precursor molecule with para -enriched hydrogen gas, and the proton singlet order in fumarate is released as antiphase NMR signals by enzymatic conversion to malate in D 2 O. Using this model system we show two pulse sequences to rephase the NMR signals for imaging and suppress the background signals from water.

The hyperpolarization-enhanced 1 H‑imaging modality presented here can allow for hyperpolarized imaging without the need for low‑abundance, low‑sensitivity heteronuclei.
Non-ionizing energy loss causes bulk damage to the silicon sensors of the ATLAS pixel and strip detectors. This damage has important implications for data-taking operations, charged-particle track reconstruction, detector simulations, and physics analysis.

This paper presents simulations and measurements of the leakage current in the ATLAS pixel detector and semiconductor tracker as a function of location in the detector and time, using data collected in Run 1 (2010–2012) and Run 2 (2015–2018) of the Large Hadron Collider.

The extracted fluence shows a much stronger | z |-dependence in the innermost layers than is seen in simulation. Furthermore, the overall fluence on the second innermost layer is significantly higher than in simulation, with better agreement in layers at higher radii. These measurements are important for validating the simulation models and can be used in part to justify safety factors for future detector designs and interventions.
МОРФОЛОГИЧЕСКАЯ ХАРАКТЕРИСТИКА ТКАНЕВОГО МИКРОРАЙОНА ЩИТОВИДНОЙ ЖЕЛЕЗЫ ПРИ ЭКСПЕРИМЕНТАЛЬНОМ ГИПОТИРЕОЗЕ

Ольга Владимировна ГОРЧАКОВА 1
MOTIVATION: A reliable recognition of transcription factor binding sites is essential for analysis of regulatory genomic sequences. The experimental data make evident an important role of DNA conformational features for site functioning. However, Internet-available tools for revealing conformational and physicochemical DNA features significant for the site functioning and subsequent use of these features for site recognition have not been developed up to now. RESULTS: We suggest an approach for revealing significant conformational and physicochemical properties of functional sites implemented in the database B-DNA-VIDEO. This database is designed to study the sets of various transcription factor binding sites, providing evidence that transcription factor binding sites are characterized by specific sets of significant conformational and physicochemical DNA properties. For a fixed site, by using the B-DNA features selected for this site recognition, the C-program recognizing this site may be generated, control tested and stored in the database B-DNA-VIDEO. Each B-DNA-VIDEO entry links to the Web-applet recognizing the site, whose significant B-DNA features are stored in this entry as the 'site recognition programs'. The pairwise linked entry-applet pairs are compiled within the B-DNA-VIDEO system, which is simultaneously the database and the program tools package applicable immediately for recognizing the sites stored in the database. Indeed, this is the novelty. Hence, B-DNA-VIDEO is the Web resource of both 'searching for static data' and 'active computation' type, that is why it was called an 'activated database'. AVAILABILITY: B-DNA-VIDEO is available at http://wwwmgs.bionet.nsc.ru/systems/BDNAVideo/ and the mirror site at http://www.cbil.upenn.edu/mgs/systems/c onsfreq/.
Nitrogen-containing multi-wall carbon nanotubes (N-MWCNTs) were synthesized using aerosol assisted chemical vapor deposition (CVD) techniques in conjunction with benzylamine:ferrocene or acetonitrile:ferrocene mixtures. Different amounts of toluene were added to these mixtures in order to change the N/C ratio of the feedstock.

X-ray photoelectron and near-edge X-ray absorption fine structure spectroscopy detected pyridinic, pyrrolic, graphitic, and molecular nitrogen forms in the N-MWCNT samples. Analysis of the spectral data indicated that whilst the nature of the nitrogen-containing precursor has little effect on the concentrations of the different forms of nitrogen in N-MWCNTs, the N/C ratio in the feedstock appeared to be the determining factor.

When the N/C ratio was lower than ca. 0.01, all four forms existed in equal concentrations, for N/C ratios above 0.01, graphitic and molecular nitrogen were dominant. Furthermore, higher concentrations of pyridinic nitrogen in the outer shells and N2 molecules in the core of the as-produced N-MWCNTs suggest that the precursors were decomposed into individual atoms, which interacted with the catalyst surface to form CN and NH species or in fact diffused through the bulk of the catalyst particles. These findings are important for a better understanding of possible growth mechanisms for heteroatom-containing carbon nanotubes (CNTs) and therefore paving the way for controlling the spatial distribution of foreign elements in the CNTs using CVD processes.
This review focuses on topological features of three-periodic (framework) p, d, and f metal cyano complexes or cyanometallates, i.e. coordination compounds, where CN– ligands play the main structure-forming role. In addition, molecular, one-periodic (chain), and two-periodic (layer) cyanometallates are considered as possible building blocks of the three-periodic cyanometallates.

All cyanometallates are treated as systems of nodes (mononuclear, polynuclear, or transitional metal cluster complexes) joined together via CN-containing spacers. The most typical nodes and spacers as well as methods of their connection are described and systematized. Particular attention is paid to the overall structural motifs in the three-periodic cyanometallates, especially to the relations between the local coordination (coordination figure) of structural units and the entire framework topology.

The chemical factors are discussed that influence the cyanometallate topological properties due to modification of nodes, spacers, or coordination figures.
This paper presents a basic formalization of university-government-industry interaction (the concept of the Triple Helix).

Such formalization is based on game theory principles and methods, as well as the methods and instruments of the theory of optimal allocation of resources.The constructed models simulate the phases of R&D, implementation and commercialization of a new project, as well as risks and uncertainty on each of these innovation stages.Based on the presented formalization, a business simulation game as a game-experimental model was developed and tested.

The paper demonstrates that game-theoretic and optimal resource solutions may serve as a benchmark for real interactions of the key stakeholders of innovation ecosystems of different scales.
A new measurement of the branching fraction of the leptonic decay ${B}^{+}\ensuremath{\rightarrow}{\ensuremath{\tau}}^{+}\phantom{\rule{0}{0ex}}{\ensuremath{\nu}}_{\ensuremath{\tau}}$ confirms previous measurements and agrees with standard model predictions.
Bahadur proved that in this case the number of different words as a function of the length of the text, again, asymptotically behaves like a power function. On the other hand, in the applied statistics community there are statements known as the Zipf's and Heaps' laws that are supported by empirical observations.

We explore a probabilistic model of an artistic text: words of the text are chosen independently of each other in accordance with a discrete probability distribution on an infinite dictionary. The words are enumerated 1, 2, ..., and the probability of appearing the i'th word is asymptotically a power function. We highlight the links between Bahadur results and Zipf's/Heaps' laws, and introduce and analyse a corresponding statistical test.

We highlight the links between Bahadur results and Zipf's/Heaps' laws, and introduce and analyse a corresponding statistical test.
This Special Issue of Minerals covers a broad range of topics related to the mineralogy of noble metals (Au, Ag, Pt, Pd, Rh, Ru) and the forms of occurrence, formation and distribution of these elements in natural ore-forming systems [...]
Vapor condensation of the HFE-7100 in loop heat pipe was studied experimentally and theoretically.

Numerical calculations of the vapor condensation on the curvilinear fin have been performed.

Numerical, theoretical and experimental data are in a good agreement. Minimal condensate film thickness on the top of the fin has been determined and increases monotonously with the increase in the temperature drop.