Hengzongshu/ArticleAgent huggingface dataset:
Multiple DNA repair pathways may be involved in the removal of the same DNA lesion caused by endogenous or exogenous agents. Although distinct DNA repair machinery fulfill overlapping roles in the repair of DNA lesions, the mechanisms coordinating different pathways have not been investigated in detail.

Here, we show that Ku70, a core protein of nonhomologous end‐joining (NHEJ) repair pathway, can directly interact with DNA polymerase‐β (Pol‐β), a central player in the DNA base excision repair (BER), and this physical complex not only promotes the polymerase activity of Pol‐β and BER efficiency but also enhances the classic NHEJ repair. Moreover, we find that DNA damages caused by methyl methanesulfonate (MMS) or etoposide promote the formation of Ku70‐Pol‐β complexes at the repair foci. Furthermore, suppression of endogenous Ku70 expression by small interfering RNA reduces BER efficiency and leads to higher sensitivity to MMS and accumulation of the DNA strand breaks. Similarly, Pol‐β knockdown impairs total‐NHEJ capacity but only has a slight influence on alternative NHEJ.

These results suggest that Pol‐β and Ku70 coordinate 2‐way crosstalk between the BER and NHEJ pathways.
In this study, we demonstrated experimentally that formation of chains and islands of oxygen vacancies in hafnium sub-oxides (HfO$_x$, $x<2$) leads to percolation charge transport in such dielectrics. Basing on the model of \'{E}fros-Shklovskii percolation theory good quantitative agreement between the experimental and theoretical data of current-voltage characteristics were achieved. Based on the percolation theory suggested model shows that hafnium sub-oxides consist of mixtures of metallic Hf nanoscale clusters of 1-2 nm distributed onto non-stoichiometric HfO$_x$. It was shown that reported approach might describe low resistance state current-voltage characteristics of resistive memory elements based on HfO$_x$.
Surface plasmon resonance (SPR) microscopy is one of the most sensitive label-free microscopy methods, however with low lateral resolution. This drawback is primarily caused by the fact that surface plasmon polaritons (SPPs) excited by terahertz (THz) radiation propagate macro distances (about ∼100 1) from their excitation spot, thereby blurring the observed region, like with a scattering medium. To eliminate this disadvantage, we adapt a method known as ghost imaging (GI), which is notable for its tolerance to environmental aberrations between the object and camera. We propose an optical scheme of SPR microscopy for the THz range with an additional optical arm to implement the classical GI and provide an analysis of factors affecting the image quality. To implement the classical GI in the THz range, we propose to use the Novosibirsk free electron laser (NovoFEL) as a THz radiation source. The high beam power of the NovoFEL provides a speckle structure with a pronounced profile, which is required to achieve a high level of contrast in the obtained image. Moreover, the large coherence length of the NovoFEL radiation is necessary to ensure a large degree of correlation between the beams emerging from the beam splitter, which allows reconstructing an image with a lower noise level.
Hoeffding-type exponential inequalities are obtained for the distribution tails of canonical von Mises' statistics of arbitrary order based on samples from a stationary sequence of random variables satisfying the {\varphi}-mixing condition.

The present paper weakens the restrictions on the coefficient {\varphi}() which are contained in the paper "Exponential inequalities for the distributions of canonical U- and V-statistics of dependent observations" by Borisov and Volodko (2009).

At the same time, we correct the corresponding proof in this paper.
Background.It is well-documented that (bio)chemical reaction capacity of raw potato starch depends on crystallinity, morphology and other chemical and physical properties of starch granules, and these properties are closely related to gene functions.Preparative yield, amylose/amylopectin content, and phosphorylation of potato tuber starch are starch-related traits studied at the genetic level.In this paper, we perform a genome-wide association study using a 22K SNP potato array to identify for the first time genomic regions associated with starch granule morphology and to increase number of known genome loci associated with potato starch yield. Methods.A set of 90 potato (Solanum tuberosum L.) varieties from the ICG "GenAgro" collection (Novosibirsk, Russia) was harvested, 90 samples of raw tuber starch were obtained, and DNA samples were isolated from the skin of the tubers.Morphology of potato tuber starch
The Internet anonymity for a long time made it difficult for journalists to use the content presented in it. Now, thanks to the convergence of the 'virtual' personality and the 'real' one, this problem has been mitigated.

The continuous selection from RIA Novosti, Life.ru and Lenta.ru (683 articles) allowed us to examine features of representation of information from social networks.

It was found that journalists use social media materials to confirm some fact or position of a person, as an information guide, they borrow there photos or videos, and, analyzing the comments, reproduce the 'opinion of the audience'. In general, it is shown that social networks have already secured the place of information providers for the media. The media began to trust this information, learned how to work with it, to process it for certain genres. There are even special software tools that allow Internet media to integrate very organically posts from social networks into their pages with publications when creating a site. The identical virtual personality of a real person, created now in social media, increasingly brings communication within the network closer to communication in real life, which expands the professional journalistic toolkit.
In aqueous solution, cyclodextrins (CDs) are able to bind strongly either hydrophobic species or also hydrophilic molecules such as octahedral hexametallic cluster.

Systematic investigation of the reactivity between native CDs (α- or β-CD) and water-soluble rhenium clusters [Re6Q8(CN)6]4– with Q = S, Se, and Te were performed, leading to six new crystal structures revealing different types of supramolecular arrangements. Solution investigations, using a set of complementary techniques including isothermal titration calorimetry, multinuclear NMR methods, cyclic voltammetry, and electrospray ionization mass spectrometry, corroborate nicely conclusions of the solid-state studies.

Encapsulation of [Re6Q8(CN)6]4– (Q = S, Se, or Te) within two β-CDs is observed regardless of the cluster size. Interestingly, different assembling scenarios are pointed out depending on the host–guest matching featured by no, partial, or deep inclusion complexes that involved either primary or secondary rim of the CD tori. In the specific case of α-CD, only the smaller cluster [Re6S8(CN)6]4– is able to form inclusion complex with the tori host. It appears clearly that size-matching supported by solvent effects play key roles in the stability of the host–guest complexes. At last, circular dichroism studies underline that the chirality induction from cyclodextrins to the rhenium cluster depends strongly on the strength of host–guest interactions.
Aging | doi:10.18632/aging.101163. Alexey Moskalev, Vladimir Anisimov, Aleksander Aliper, Artem Artemov, Khusru Asadullah, Daniel Belsky, Ancha Baranova, Aubrey de Grey, Vishwa Deep Dixit, Edouard Debonneuil, Eugenia Dobrovolskaya, Peter Fedichev, Alexander Fedintsev, Vadim Fraifeld, Claudio Franceschi, Rosie Freer, Tamas Fülöp, Jerome Feige, David Gems, Vadim Gladyshev, Vera Gorbunova, Irina Irincheeva, Sibylle Jager, S. Michal Jazwinski, Matt Kaeberlein, Brian Kennedy, Daria Khaltourina, Igor Kovalchuk, Olga Kovalchuk, Sergey Kozin, Alexander Kulminski, Ekaterina Lashmanova, Ksenia Lezhnina, Guang-Hui Liu, Valter Longo, Polina Mamoshina, Alexander Maslov, Joao Pedro de Magalhaes, James Mitchell, Arnold Mitnitski, Yuri Nikolsky, Ivan Ozerov, Elena Pasyukova, Darya Peregudova, Vasily Popov, Ekaterina Proshkina, Evgeny Putin, Evgeny Rogaev, Blanka Rogina, Jane Schastnaya, Andrey Seluanov, Mikhail Shaposhnikov, Andreas Simm, Vladimir Skulachev, Maxim Skulachev, Ilya Solovev, Stephen Spindler, Natalia Stefanova, Yousin Suh, Andrew Swick, John Tower, Andrei V. Gudkov, Jan Vijg, Andrey Voronkov, Michael West, Wolfgang Wagner, Anatoliy Yashin, Nadezhda Zemskaya, Zhaxybay Zhumadilov, Alex Zhavoronkov
Downstream development of artificial disturbances were investigated experimentally using hot-wire constant temperature anemometry. It is shown that vibrations with high-amplitude of a three-dimensional surface lead to formation of two types of perturbations in the straight wing boundary layer: streamwise oriented localized structures and wave packets. The amplitude of streamwise structure is decay downstream. The wave packets amplitude grows in adverse pressure gradient area. The flow separation is exponentially intensified of the wave packet amplitude.
Thermal decomposition of alkoxyamines in the presence of scavengers was found to proceed with the formation of chemically induced nuclear polarization detected by 1H NMR.

The distinctive Chemically Induced Dynamic Nuclear Polarization (CIDNP) features were studied using the example of three alkoxyamines: 4-nitrophenyl 2-(2,2,6,6-tetramethylpiperidin-1-yloxy)-2-methylpropanoate (1a), 4-nitrophenyl 2-(2,2-diphenyl-3-phenylimino-2,3-dihydroindol-1-yloxy)-2-methylpropanoate (2a) and 4-nitrophenyl 2-(2,2,5,5-tetramethyl-4-phenyl-2H-imidazol-1-oxy)-2-methylpropanoate (3a) in the presence of PhSH. The analysis of CIDNP signs of methacrylate protons allows us to conclude on the occurrence of hydrogen atom transfer reaction in geminate radical pair formed in alkoxyamine thermolysis.

Thus, CIDNP is a fast and sensitive method to detect the occurrence of intra/intermolecular hydrogen transfer in alkoxyamine thermolysis.
Anthracycline antibiotics are an important group of widely used and highly eff ective antitumor drugs. However, the cardiotoxicity of anthracyclines included in the polychemotherapy regimens results in the development of cardiovascular diseases both in the early and late rehabilitation period. The article presents a clinical case demonstrating the long-term consequences of highdose polychemotherapy with the onset of heart failure and coronary artery disease.
The thermoelastic parameters of pure knorringite were estimated and were compared with the previous data on other garnet compositions.

The P‐V‐T equation of state (EoS) for majoritic knorringite Mg 3.19 Cr 1.60 Si 3.19 O 12 at pressures to 17 GPa and temperatures to 1673 K was obtained from in situ X‐ray diffraction experiments using a Kawai‐type multi‐anvil apparatus. Fitting of the room‐temperature P–V data to a third‐order Birch‐Murnaghan EoS yielded an isothermal bulk modulus, K 0,300 = 154 (4) GPa, and a pressure derivative, K ′ 0,300 = 5.4 (1.2). When fitting a high‐temperature Birch‐Murnaghan EoS using all of the P‐V‐T data at a fixed V 0 = 1549.08 Å 3 , we find that K 0,300 = 157 (2) GPa, K ′ 0, T = 4.5 (6), (∂ K 0, T /∂ T ) P = −0.019 (4) (GPa K −1 ), a = 3.00 (14) × 10 −5 K −1 , and b = 0.65 (24) × 10 −8 K −2 , where α = a + bT is the volumetric thermal expansion coefficient. Fitting the Mie‐Grüneisen‐Debye EoS with the present data with a Debye temperature fixed at θ 0 = 731 K yielded a Grüneisen parameter, γ 0 = 1.34 at q = 1.0 (fixed).

The presence of Cr 2 O 3 in pyrope garnets in the upper mantle decreases P‐ and S‐velocities by 1.6% and the density increases by 1.7% (for 20 mol.% knorringite end member) compared to pure pyrope. The results show the importance of accounting knorringite end‐member for accurate estimation of mantle garnet acoustic velocities.
One of the highly debatable issue of the Altai Pleistocene paleogeography is the chronology and extension of the last ice-dammed lakes. For the first time paleontological, mineralogical characteristics, and optically stimulated luminescence (OSL) ages for lacustrine deposits confirm accumulation of ice-dammed lake with depth at least 170 m in Kurai basin at the end of the Late Pleistocene - 16-19 ka. New data provide information about the ecology and evolution of this Sartan reservoir. Draining of this lake less affected the topography in comparison with earlier cataclysmic glacier-lakes outburst floods. Nevertheless, the lake controlled human settlement of the basin in the Late Paleolithic. Drying of this reservoir defined formation of the modern hydrological network, including flowing of the Chuya River along the steep gorge between the Maashey and Belgibash mouths. New data evidence for significant difference in evolution of the Kurai-Chuya system of intermountain depressions and Uimon basin at the end of the Late Pleistocene.
The fungal pathogen Cochliobolus sativus Drechs. Ex Dastur, anamorph Bipolaris sorokiniana (Sacc.) Shoemaker is one of the most common barley pathogens worldwide and causes spot blotch and root rot in barley. Spot blotch is considered to be the major biotic stress hampering the commercial production of barley. During high disease severity, which occurs in the northwestern region of Russia once every three to four years, yield losses for barley may reach 40%. An increase in common root rot severity results in yield losses that can reach 80%.

The goal of the current study was to identify significant markers that can be employed as diagnostic DNA markers to breed C. sativus pathogen-resistant varieties of barley. In 94 spring barley cultivars and lines, the resistance of seedlings and adult plants to the impact of C. sativus on their leaves and roots was investigated.

Five genomic regions associated with resistance to Spot blotch were identified (on chromosome 1H (50–61.2 cM), 2H (68.7–69.68 cM), 3H (18.72–26.18 cM), 7H (7.52–15.44 cM)). No significant loci were determined to be associated with root rot. According to obtained data, 11 significant SNPs were converted into KASP markers and 6 markers located on chromosome 3H were determined to possess good accuracy and the potential to be employed in marker-assisted selection.
Calcification is a very common phenomenon in the coronary arteries, which is part of the atherosclerotic process, and the degree of calcification can predict clinical outcomes in patients at high risk of coronary events. Both the degree of calcification and the patterns of its distribution are of prognostic importance, but the relationship of coronary artery calcification with atherosclerotic plaque instability is extremely complex and not fully understood.

This article is devoted to the study of calcification markers and their influence on the development of atherosclerotic foci.
Despite the fact that most young patients with hyperglycemia are diagnosed with type 1 (T1DM) and type 2 (T2DM) diabetes, up to 10 % of all cases of the disease are MODY diabetes. The most common types of MODY are GCK-MODY and HNF1A-MODY, therefore the investigation of their clinical and laboratory characteristics, including lipid spectrum indicators is of high clinical significance. The aim of this research work was to study the values of lipid spectrum indicators in patients diagnosed with GCK-MODY and HNF1A-MODY at the age from 18 to 45 years. Lipid profile parameters were investigated in 56 patients aged 18 to 45 years with diagnosed GCK-MODY and HNF1A-MODY by molecular genetic tests, matched by sex, age and body mass index (BMI). No statistically significant differences were found for any of the indicators, however, in patients with HNF1A-MODY, the decrease in HDL-C is determined significantly more often than in GCKMODY. Thus, the group of persons with MODY differs in the level of lipid profile indices depending on the type of MODY.
This paper analyzes the problems of designing enterprise systems for solving problems with several sections and types of accounting. The particular problem of the interaction of the operational and accounting circuits is solved by the example of work with tolling raw materials. The necessity of creating a model for the interaction of different subsystems that ensure the correctness of work with transactions at the level of business logic is indicated. An appropriate model is proposed that allows design integration solutions based on several subsystems with a description of the exchange of transactional data.
ABSTRACT There are many psychiatric medications targeting the activity of SLC transporters. Therefore, further research is needed to elucidate the expression profiles of the Slc* genes, which may serve as markers of altered brain metabolic processes and neurotransmitter activities in psychoneurological disorders. We studied differentially expressed Slc genes using the transcriptomic profiles in the ventral tegmental area (VTA), nucleus accumbens (NAcc), and prefrontal cortex (PFC) of male mice with psychosis-like behavior induced by repeated aggression experience in daily agonistic interactions which are accompanied by wins. Most of differentially expressed Slc genes in the VTA and NAcc (12 of 17 and 25 of 26, respectively) were downregulated, which was not the case in the PFC (6 and 5, up- and down, respectively). Also, the majority of these genes were shown to have brain region-specific expression profiles. In the VTA and NAcc altered expression was observed for the genes encoding the transporters of neurotransmitters as well as inorganic and organic ions, amino acids, metals, glucose, etc . This means alteration in transport functions for many substrates, which results in complete disruption of all cellular and neurotransmitter processes. The neurotransmitter systems, especially, the dopaminergic one, in male mice with positive fighting experience in daily agonistic interactions undergo changes leading to profound genomic modifications which include downregulated expression of the majority of the Slc* genes at least in the VTA and NAcc, which is attributable to chronic stimulation of the reward systems.
Fifty-three species of perennial grasses in the genus Elymus L. (Poaceae), which are widespread in Russia, are generally assumed to have three haplome combinations: StH, StY and StHY. The StH-genome species, endemic to Russia, remain the least studied. R. Mason-Gamer and co-authors have previously shown in a series of studies that a molecular phylogenetic analysis of the low-copy gene waxy (GBSS1) sequences significantly complements cytogenetic data on the genomic constitution and evolutionary relationships among both North American and Asian species of the genus Elymus. To determine the species’ genomic constitution and to evaluate the level of phylogenetic differentiation, we examined the GBSS1 gene in 18 species of Elymus from Siberia and the Russian Far East, including the fol- lowing 14 endemics: E. charkeviczii, E. jacutensis, E. kamczadalorum, E. komarovii, E. kronokensis, E. lenensis, E. macrourus, E. margaritae, E. subfibrosus, E. sajanensis, E. transbaicalensis, E. peschkovae, E. uralensis, and E. viridiglumis.PCR amplification products of GBSS1 gene fragments (including exons 9–14) were cloned and 6–8 clones per accession were sequenced. It appears that all the species studied have St and H subgenomic gene variations. The most significant differences between the subgenomic variants St and H were found in intron 13. The H subgenome contains a 21-bp-long deletion in intron 13 in all Elymus genotypes, probably derived from a common ancestor of the H and P genomes. Instead of this deletion, all St subgenomes have a relatively conservative sequence similar to that of the genus Pseudoroegneria, whose ancestor is considered to be the donor of the modern St subgenome for all Elymus species. Cluster phylogenetic analysis revealed differentiation in St and H subgenome sequences into two evolutionary variants: St1 vs. St2 and H1 vs. H2, respectively. Variants of the St and H subgenomes were found homologous to various modern species of the ancestral genera Pseudoroegneria and Hordeum: St1 to P. strigosa, St2 to P. spicata, H1 to H. jubatum, and H2 to H. californicum. The details of the relationships between Russian and North American species of the genus, as well as a number of microevolutionary interconnections in the group of boreal endemic species of Siberia and the Russian Far East were revealed. The new results obtained here are essential for the development of a phylogenetically oriented taxonomic system for the genus Elymus.
The linear model for «Yamal LNG» is estimated using DCF method. The hypothetical model without PPP mechanisms is also considered in the article.

The linear estimation is not enough for a huge long-term project. Therefore, the risk analysis is carried out in the article. The positive effect on region development is shown in order to estimate social sides of the project realization.

As a result, we get project’s NPV of $1,337 blns, which would be true with 49,3% likelihood. The denial of PPP mechanisms leads to $-7,1 blns NPV.
The research was performed on the gas-dynamic complex of experimental installations located in the Department of Applied Physics of Novosibirsk State University.

Spectral methods and molecular beam mass spectrometry were used to diagnose the processes. A significant difference in the clustered argon mass spectra was found in comparison of spectrum recorded during ionization by a quadrupole mass spectrometer own ionizer and spectrum obtained with the transport of ions formed by an electron beam in the jet through a skimmer to the mass spectrometer detector (without using an ionizer of spectrometer).

The second variant of gas flow ionization presents the possibility of registering the mass spectrum of a hydrocarbon series with features indicating the occurrence of heavier hydrocarbons in the jet. The mechanism of intracluster energy exchange with the transfer of excitation initiated by electrons with subsequent intense radiation at certain wavelengths of a neutral argon atom was detected and identified.
In the T(1;2)dorvar7 translocation, the 1A-2B7-8 segment of the X chromosome is brought to the vicinity of 2R-chromosome heterochromatin resulting in position effect variegation of dor, BR-C and more distal genes, as well as compaction of chromatin in this segment.

By irradiation of T(1;2)dorvar7, nine reversions (rev) to a normal phenotype were recovered. In two cases (rev27, rev226), the 1A-2B7-8 section is relocated to the 19A region of the X chromosome, forming free duplications (1A-2B7-8/19A-20F-X-het). Modifiers of position effect do not change the normal expression of the BR-C and dor genes in these duplications. In five reversions (rev3, rev40, rev60, rev167, rev175), free duplications have formed from the 1A-2B7-8 fragment and X chromosome heterochromatin. In these rearrangements, modifiers of position effect (low temperature, removal of Y and 2R-chromosome heterochromatin and a genetic enhancer (E-var(3)201) induce position-effect again. Two reversions (rev45 and rev110) are associated with additional inversions in the original dorvar7 chromosomes. The inversions relocate part of the heterochromatin adjacent to the 1A-2B7-8 section into new positions. In T(1;2)dorrev45, position-effect is seen in the 2B7-8-7A element as compaction spreading from 2B7-8 proximally in some cases as far as the 5D region.

Thus, in rev45 the pattern of euchromatin compaction is reciprocal to that of the initial dorvar7 strain. Apparently, it is due to the same variegation-evoking center near the 2R centromere in both cases. In all nine revertants, weakening or complete disappearance of the position-effect is observed despite retention of the 20-kb heterochromatic segment adjacent to the 1A-2B7-8 region. Thus, a 20-kb heterochromatic sequence does not inactivate euchromatin joined to it.