Hengzongshu/ArticleAgent huggingface dataset:
Corticosteroids are widely used in pediatric cardiac surgery to blunt systemic inflammatory response and to reduce complications; nevertheless, their clinical efficacy is uncertain.

To determine whether intraoperative administration of dexamethasone is more effective than placebo for reducing major complications and mortality during pediatric cardiac surgery.The Intraoperative Dexamethasone in Pediatric Cardiac Surgery was an investigator-initiated, double-blind, multicenter randomized trial that involved 4 centers in China, Brazil, and Russia. A total of 394 infants younger than 12 months, undergoing cardiac surgery with cardiopulmonary bypass were enrolled from December 2015 to October 2018, with follow-up completed in November 2018.The dexamethasone group (n = 194) received 1 mg/kg of dexamethasone; the control group (n = 200) received an equivolume of 0.9% sodium chloride intravenously after anesthesia induction.The primary end point was a composite of death, nonfatal myocardial infarction, need for extracorporeal membrane oxygenation, need for cardiopulmonary resuscitation, acute kidney injury, prolonged mechanical ventilation, or neurological complications within 30 days after surgery. There were 17 secondary end points, including the individual components of the primary end point, and duration of mechanical ventilation, inotropic index, intensive care unit stay, readmission to intensive care unit, and length of hospitalization.

All of the 394 patients randomized (median age, 6 months; 47.2% boys) completed the trial. The primary end point occurred in 74 patients (38.1%) in the dexamethasone group vs 91 patients (45.5%) in the control group (absolute risk reduction, 7.4%; 95% CI, -0.8% to 15.3%; hazard ratio, 0.82; 95% CI, 0.60 to 1.10; P = .20). Of the 17 prespecified secondary end points, none showed a statistically significant difference between groups. Infections occurred in 4 patients (2.0%) in the dexamethasone group vs 3 patients (1.5%) in the control group.Among infants younger than 12 months undergoing cardiac surgery with cardiopulmonary bypass, intraoperative administration of dexamethasone, compared with placebo, did not significantly reduce major complications and mortality at 30 days. However, the study may have been underpowered to detect a clinically important difference.
Auxin responsive elements (AuxRE) were found in upstream regions of target genes for ARFs (Auxin response factors). While Chip-seq data for most of ARFs are still unavailable, prediction of potential AuxRE is restricted by consensus models that detect too many false positive sites.

Using sequence analysis of experimentally proven AuxREs, we revealed both an extended nucleotide context pattern for AuxRE itself and three distinct types of its coupling motifs (Y-patch, AuxRE-like, and ABRE-like), which together with AuxRE may form the composite elements. Computational analysis of the genome-wide distribution of the predicted AuxREs and their impact on auxin responsive gene expression

allowed us to conclude that: (1) AuxREs are enriched around the transcription start site with the maximum density in 5'UTR; (2) AuxREs mediate auxin responsive up-regulation, not down-regulation. (3) Directly oriented single AuxREs and reverse multiple AuxREs are mostly associated with auxin responsiveness. In the composite AuxRE elements associated with auxin response, ABRE-like and Y-patch are 5'-flanking or overlapping AuxRE, whereas AuxRE-like motif is 3'-flanking. The specificity in location and orientation of the coupling elements suggests them as potential binding sites for ARFs partners.
Aim. To study inheritance patterns of atrial fibrillation (AF) and association of primary and secondary AF with gene polymorphism of β1-adrenoreceptors. Material and methods. 103 probands with AF and their 301 relatives of I, II, III degrees (basic group) and 82 probands without heart diseases and their 163 relatives of I and II degrees (control group) were examined. Examination included evaluation of electrophysiological indicators of sinoatrial node, electrocardiogram monitoring, veloergometry, echocardiography as well as assessment of gene polymorphism of β1-adrenoretseptors. Results. Accumulation of AF in probands families was founded. Segregation analysis of idiopathic AF revealed autosomal-dominant type of its inheritance. Сonclusion. The heterozygote genotype of gene β1-adrenoretseptors Ser49Gly is one of genetic predictors of primary and secondary AF.
The work is devoted to the calculation of gas flow and modeling of droplet sedimentation of medicinal aerosols in human lungs. To solve this problem, a previously constructed three-dimensional model of a human bronchial tree was used.

For calculation of gas flow in lungs and aerosol deposition the method of step-by-step calculation of the flow in bronchial tree was used. It allowed to calculate the air flow and aerosol droplets deposition in all bronchial branches up to alveolus. Human breathing was calculated using COVID-19 thermal helium-oxygen mixture treatment method. Breathing with a thermal helium-oxygen mixture requires less effort from the patient than breathing with air. Three-dimensional numerical calculations of air flow and aerosol droplets in human lungs have been performed. The characteristics of a serial medical nebulizer were used to calculate the deposition of aerosol droplets.

It is shown that the number of past droplets depends (except for flow, droplet size) on the duration of breath. The nebulizer under consideration does not provide delivery of medicines to the lung alveolus.
Observations and numeric modeling of internal wave generation and transformation in the shelf zone of sea show that the main part of tidal energy is transported to shores in form of internal gravitational waves. Long-term measurements of temperature and current velocity fluctuations at many levels in the near-bottom thermocline were carried out during the periods when stable seasonal thermocline was present. Analysis of the measurements permits us to understand mechanisms of internal wave destruction with turbulent motion generation and corresponding rebuilding of velocity and density mean fields in the stratified near-bottom layer. Spectral analysis of temperature fluctuations shows that in shoaling internal waves the low-frequency maxima disappear, maxima at higher frequencies appear, and the spectra slope in the high frequency range changes with depth. Taking into account the concurrent analysis of near-bottom pressure fluctuations and current velocity fluctuations from surface till bottom we come to the conclusion that breaking internal waves in a near-bottom thermocline generate not only small-scale three-dimensional turbulence, but also quasi-horizontal turbulence of larger scales, which considerably contributes into mixing and sediments, alluvium, and nutrients transport in the shelf zone of sea.
无明确相关研究描述

2017年在俄罗斯新西伯利亚地区进行的调查，收集了约600个Dolichopodidae标本

几乎所有44个已鉴定物种都是首次在新西伯利亚地区记录
We describe experimental and theoretical premises of a powerful cancer therapy based on the combination of three approaches. These include (I) in situ vaccination (intratumoral injections of CpG oligonucleotides and anti-OX40 antibody); (II) chronometric or metronomic low-dose cyclophosphamide (CMLD CP)-based chemotherapy; (III) cancer stem cell-eradicating therapy referred to as Karanahan (from the Sanskrit kāraṇa ['source'] + han ['to kill']).In murine models, the first two approaches are particularly potent in targeting immunogenic tumors for destruction. In situ vaccination activates a fully fledged anticancer immune response via an intricate network of ligand-receptor-cytokine interactions. CMLD CP-based chemotherapy primarily targets the suppressive tumor microenvironment and activates tumor-infiltrating effectors. In contrast, Karanahan technology, being aimed at replicative machinery of tumor cells (both stem-like and committed), does not depend on tumor immunogenicity. With this technology, mice engrafted with ascites and/or solid tumors can be successfully cured. There is a significant degree of mechanistic and therapeutic overlap between these three approaches. For instance, the similarities shared between in situ vaccination and Karanahan technology include the therapeutic procedure, the cell target [antigen-presenting cells (APC) and dendritic cells (DC)], and the use of DNA-based preparations (CpG and DNAmix). Features shared between CMLD CP-based chemotherapy and Karanahan technology are the timing and the dose of the cytostatic drug administration, which lead to tumor regression.The following keywords were used to search PubMed for the latest research reporting successful eradication of transplantable cancers in animal models that relied on approaches distinct from those used in the Karanahan technology: eradication of malignancy, cure cancer, complete tumor regression, permanently eradicating advanced mouse tumor, metronomic chemotherapy, in situ vaccination, immunotherapy, and others.

We hypothesize, therefore, that very potent anticancer activity can be achieved once these three therapeutic modalities are combined into a single approach.

This multimodal approach is theoretically curative for any type of cancer that depends on the presence of tumor-inducing cancer stem cells, provided that the active therapeutic components are efficiently delivered into the tumor and the specific biological features of a given patient's tumor are properly addressed. We expect this multimodal approach to be primarily applicable to late-stage or terminal cancer patients who have exhausted all treatment options as well as patients with inoperable tumors.
B chromosomes (Bs) or supernumerary chromosomes are extra chromosomes in the species karyotype that can vary in its copy number. Bs are widespread in eukaryotes. Usually, the Bs of specimens collected from natural populations are the object of the B chromosome studies. We applied another approach analyzing the Bs in animals maintained under the laboratory conditions as lines and cultures. In this study, three species of the Macrostomum genus that underwent a recent whole-genome duplication (WGD) were involved. In laboratory lines of M. lignano and M. janickei, the frequency of Bs was less than 1%, while in the laboratory culture of M. mirumnovem, it was nearer 30%. Their number in specimens of the culture varied from 1 to 14. Mosaicism on Bs was discovered in parts of these animals. We analyzed the distribution of Bs among the worms of the laboratory cultures during long-term cultivation, the transmission rates of Bs in the progeny obtained from crosses of worms with different numbers of Bs, and from self-fertilized isolated worms. The DNA content of the Bs in M. mirumnovem was analyzed with the chromosomal in situ suppression (CISS) hybridization of microdissected DNA probes derived from A chromosomes (As). Bs mainly consisted of repetitive DNA. The cytogenetic analysis also revealed the divergence and high variation in large metacentric chromosomes (LMs) containing numerous regions enriched for repeats. The possible mechanisms of the appearance and evolution of Bs and LMs in species of the Macrostomum genus were also discussed.
Plant expression systems are currently regarded as promising alternative platforms for the production of recombinant proteins, including the proteins for biopharmaceutical purposes. However, the accumulation level of a target protein in plant expression systems is still rather low compared with the other existing systems, namely, mammalian, yeast, and E. coli cells. To solve this problem, numerous methods and approaches have been designed and developed. At the same time, the random nature of the distribution of transgenes over the genome can lead to gene silencing, variability in the accumulation of recombinant protein, and also to various insertional mutations. The current research study considered inserting target genes into pre-selected regions of the plant genome (genomic "safe harbors") using the CRISPR/Cas system. Regions of genes expressed constitutively and at a high transcriptional level in plant cells (housekeeping genes) that are of interest as attractive targets for the delivery of target genes were characterized. The results of the first attempts to deliver target genes to the regions of housekeeping genes are discussed. The approach of "euchromatization" of the transgene integration region using the modified dCas9 associated with transcription factors is considered. A number of the specific features in the spatial chromatin organization allowing individual genes to efficiently transcribe are discussed.
Damage of divertor tungsten tiles as a result of transient plasma processes is one of the key problems of the ITER tokamak. Even if the temperature of the divertor tiles is maintained below the melting threshold, their thermal deformation, accumulation of residual stresses and cracking will occur. Cracking reduces the resistance of the metal to subsequent loads, therefore, the study of this process is important. In materials science, non-destructive methods for measuring mechanical stresses are very limited.

In this work, a new method is proposed that allows non-contact investigation of stresses in polycrystalline tungsten plates by their bending. A corresponding diagnostic system and theoretical model are implemented. A number of experiments were carried out using this diagnostic system at the BETA facility at the Budker Institute to study the dynamics of tungsten plates bending with different thickness, subjected to pulsed heating of different intensities.

The experimental results showed the qualitatively expected process of the plate bending, as well as good agreement with the theoretical model. This proved that the method described in the work is promising for use in order to study the residual stresses in a pulse-heated metal sample.
// Arnaud J. Legrand 1, * , Mattia Poletto 1, * , Daniela Pankova 1 , Elena Clementi 2 , John Moore 1 , Francesc Castro-Giner 3 , Anderson J. Ryan 1 , Eric O'Neill 1 , Enni Markkanen 2, # and Grigory L. Dianov 1, 4, 5, # 1 CRUK & MRC Oxford Institute for Radiation Oncology, University of Oxford, Department of Oncology, Old Road Campus Research Building, OX37DQ Oxford, UK 2 Institute of Pharmacology and Toxicology, Vetsuisse Faculty, University of Zürich, Zürich 8057, Switzerland 3 Functional Genomics Center Zürich, University of Zürich, Zürich 8057, Switzerland 4 Institute of Cytology and Genetics, Russian Academy of Sciences, Novosibirsk 630090, Russian Federation 5 Novosibirsk State University, Novosibirsk 630090, Russian Federation * Co-first author # Co-senior author Correspondence to: Grigory L. Dianov, email: grigory.dianov@oncology.ox.ac.uk Enni Markkanen, email: enni.markkanen@vetpharm.uzh.ch Keywords: tumour microenvironment; cancer-associated fibroblasts; base excision repair; tumour stroma; midostaurin Received: October 11, 2017 Accepted: January 30, 2018 Published: February 07, 2018 ABSTRACT Cancer-associated fibroblasts (CAFs) are an emerging target for cancer therapy as they promote tumour growth and metastatic potential. However, CAF targeting is complicated by the lack of knowledge-based strategies aiming to selectively eliminate these cells. There is a growing body of evidence suggesting that a pro-inflammatory microenvironment (e.g. ROS and cytokines) promotes CAF formation during tumorigenesis, although the exact mechanisms involved remain unclear. In this study, we reveal that a prolonged pro-inflammatory stimulation causes a de facto deficiency in base excision repair, generating unrepaired DNA strand breaks and thereby triggering an ATF4-dependent reprogramming of normal fibroblasts into CAF-like cells. Based on the phenotype of in vitro -generated CAFs, we demonstrate that midostaurin, a clinically relevant compound, selectively eliminates CAF-like cells deficient in base excision repair and prevents their stimulatory role in cancer cell growth and migration.
基于在KEKB不对称能量e+e-对撞机上Belle实验收集的711 fb⁻¹数据，在Υ(4S)共振附近进行测量

使用772百万BB事件样本，其中一个B介子在强子衰变模式中完全重建，在剩余信号B子代中搜索η介子的两个衰变通道(η→γγ和η→π⁺π⁻π⁰)，并通过η'→ηπ⁺π⁻重建η'介子，结合两种η模式并使用扩展最大似然法

B⁺→ηℓ⁺νℓ的分支比测量结果为(4.2±1.1(stat.)±0.3(syst.))×10⁻⁵，对于B⁺→η'ℓ⁺νℓ，未观察到显著信号，在90%置信水平下设置上限0.72×10⁻⁴
A recent MRI method, fast macromolecular proton fraction (MPF) mapping, was used to quantify demyelination in the transient middle cerebral artery occlusion (MCAO) rat stroke model. MPF and other quantitative MRI parameters (T 1 , T 2 , proton density, and apparent diffusion coefficient) were compared with histological and immunohistochemical markers of demyelination (Luxol Fast Blue stain, (LFB)), neuronal loss (NeuN immunofluorescence), axonal loss (Bielschowsky stain), and inflammation (Iba1 immunofluorescence) in three animal groups ( n = 5 per group) on the 1st, 3rd, and 10th day after MCAO. MPF and LFB optical density (OD) were significantly reduced in the ischemic lesion on all days after MCAO relative to the symmetrical regions of the contralateral hemisphere. Percentage changes in MPF and LFB OD in the ischemic lesion relative to the contralateral hemisphere significantly differed on the first day only. Percentage changes in LFB OD and MPF were strongly correlated (R = 0.81, P < 0.001) and did not correlate with other MRI parameters. MPF also did not correlate with other histological variables. Addition of T 2 into multivariate regression further improved agreement between MPF and LFB OD (R = 0.89, P < 0.001) due to correction of the edema effect. This study provides histological validation of MPF as an imaging biomarker of demyelination in ischemic stroke.
Facile and high-yielding procedures for synthesis of monocarboxylic acid derivatives of triarylmethyl radicals (TAMs) were developed. Reaction of methyl thioglycolate with tris(2,3,5,6-tetrathiaaryl)methyl cation smoothly afforded the monosubstituted TAM derivative, which was hydrolyzed to a monocarboxylic acid, with the TAM moiety attached to thioglycolic acid via the sulfur atom. Alternatively, the diamagnetic tricarboxylic acid precursor of Finland trityl was transformed to a trimethyl ester and partially hydrolyzed under controlled conditions. The diester product was isolated and the remaining fractions were converted back to the trimethyl ester for production of more diester. The first representatives of TAM biradicals with different TAM cores and interspin distances were obtained by reaction of these new TAM monocaboxylic acids with N,N'-dimethylethylenediamine.
The paper discusses generation of volatile-bearing plumes in the mantle transition zone (MTZ) in terms of mineral-fluid petrology and their related formation of numerous localities of intra-plate bimodal volcanic series in Central and East Asia. The plume generation in the MTZ can be triggered by the tectonic erosion of continental crust at Pacific-type convergent margins and by the presence of water and carbon dioxide in the mantle. Most probable sources of volatiles are the hydrated/carbonated sediments and basalts and serpentinite of oceanic slabs, which can be subducted down to the deep mantle. Tectonic erosion of continental crust supplies crustal material enriched in uranium and thorium into the mantle, which can serve source of heat in the MTZ. The heating in the MTZ induces melting of subducted slabs and continental crust and mantle upwelling, to produce OIB-type mafic and felsic melts, respectively.
Glycosylation is a common post-translational modification of proteins. Glycosylation is associated with a number of human diseases. Defining genetic factors altering glycosylation may provide a basis for novel approaches to diagnostic and pharmaceutical applications.

Here we report a genome-wide association study of the human blood plasma N-glycome composition in up to 3811 people measured by Ultra Performance Liquid Chromatography (UPLC) technology. Starting with the 36 original traits measured by UPLC, we computed an additional 77 derived traits leading to a total of 113 glycan traits. We studied associations between these traits and genetic polymorphisms located on human autosomes.

We discovered and replicated 12 loci. This allowed us to demonstrate an overlap in genetic control between total plasma protein and IgG glycosylation. The majority of revealed loci contained genes that encode enzymes directly involved in glycosylation (FUT3/FUT6, FUT8, B3GAT1, ST6GAL1, B4GALT1, ST3GAL4, MGAT3 and MGAT5) and a known regulator of plasma protein fucosylation (HNF1A). However, we also found loci that could possibly reflect other more complex aspects of glycosylation process. Functional genomic annotation suggested the role of several genes including DERL3, CHCHD10, TMEM121, IGH and IKZF1. The hypotheses we generated may serve as a starting point for further functional studies in this research area.
The PL band in AlN was attributed to donor-acceptor (DA) transitions. At the lowest Al content, the impurity band merges with the conduction band and DA transitions are replaced by electron-acceptor transitions involving the same acceptor.

The photoluminescence properties of the intensive defect-related emission in heavily doped AlxGal-xN:Si layers with x > 0.5 have been investigated by photoluminescence (PL) spectroscopy.

The energy structure of recombination centers was obtained using the model of configuration coordinates for Al0.67Ga0.33N.
This paper was focused on studying of turbulent mixing in a model gas-turbine combustor by using planar laser-induced fluorescence. The case of model fuel injection through the pilot jet with Re = 30 000 was considered. Acetone was used as a fluorescent tracer. Data processing methods including correction of sensor sensitivity, background signal correction and correction of non-uniformity of the laser sheet were described. The instantaneous and averaged concentration fields and the concentration pulsations were demonstrated.
To cause a pandemic, an influenza virus has to overcome two main barriers. First, the virus has to be antigenically new to humans. Second, the virus has to be directly transmitted from humans to humans. Thus, if the avian influenza virus is able to pass the second barrier, it could cause a pandemic, since there is no immunity to avian influenza in the human population. To determine whether the adaptation process is ongoing, analyses of human sera could be conducted in populations inhabiting regions where pandemic virus variant emergence is highly possible. This study aimed to analyze the sera of Vietnamese residents using hemagglutinin inhibition reaction (HI) and microneutralization (MN) with A/H5Nx (clade 2.3.4.4) influenza viruses isolated in Vietnam and the Russian Federation in 2017–2018. In this study, we used sera from 295 residents of the Socialist Republic of Vietnam collected from three groups: 52 samples were collected from households in Nam Dinh province, where poultry deaths have been reported (2017); 96 (2017) and 147 (2018) samples were collected from patients with somatic but not infectious diseases in Hanoi. In all, 65 serum samples were positive for HI, at least to one H5 virus used in the study. In MN, 47 serum samples neutralizing one or two viruses at dilutions of 1/40 or higher were identified. We postulate that the rapidly evolving A/H5Nx (clade 2.3.4.4) influenza virus is possibly gradually adapting to the human host, insofar as healthy individuals have antibodies to a wide spectrum of variants of that subtype.