Hengzongshu/ArticleAgent huggingface dataset:
The in vitro cellular models are promising tools for studying normal and pathological conditions. One of their important applications is the development of genetically engineered biosensor systems to investigate the processes occurring in living cells in real time. Today, there are fluorescence protein based sensory systems for detecting various substances in living cells (for example, hydrogen peroxide, ATP, Ca 2+ etc.) or for detecting processes such as endoplasmic reticulum stress. Such systems help to study mechanisms underlying the pathogenic processes and diseases and for screening potential therapeutic compounds.

It is also necessary to develop new tools for processing and analysis of obtained microimages. Here we present our web-application CellCountCV for automation of microscopy cell images analysis which is based on fully-convolutional deep neural networks. This approach can efficiently deal with non-convex overlapping objects, that are virtually inseparable with conventional image processing methods.

The cell counts predicted with CellCountCV were very close to expert estimates (the average error rate was < 4%). CellCountCV was used to analyse large series of microscopy images obtained in experimental studies and it was able to demonstrate the endoplasmic reticulum stress development and to catch the dose-dependent effect of tunicamycin.
The early Paleozoic Terskey Suture zone, located in the southern part of the Northern Tien Shan domain in Kyrgyzstan, comprises tectonic slivers of dismembered ophiolites and associated primitive volcanics and deep-marine sediments. In the Lake Songkul area, early-middle Cambrian pillow basalts are crosscut by the Songkultau intrusion of coarse-grained gneissose quartz diorites and tonalites with geochemical characteristics typical for high-SiO2 adakites (SiO2 ​> ​56 ​wt.%, Al2O3 ​> ​15 ​wt.%, Na2O ​> ​3.5 ​wt.% and high Sr/Y and La/Yb ratios). The Songkultau granitoids have positive initial εNd (+3.8 to +6.4) and εHf (+12.3 to +13.5) values indicating derivation from sources with MORB-like isotopic signature. Volcanic formations, surrounding the Songkultau intrusion, have geochemical affinities varying from ocean floor to island arc series. This rock assemblage is interpreted as a relic of an early–middle Cambrian primitive arc where the adakite-like granitoids were derived from partial melting of young and hot subducted oceanic crust. An age of 505 ​Ma, obtained for the Songkultau intrusion, shows that hot subduction under the Northern Tien Shan continued until middle Cambrian. The primitive arc complexes were obducted onto the Northern Tien Shan domain, where the Andean type continental magmatic arc developed in Cambrian and Ordovician. Formation of the Andean type arc was accompanied by uplift, erosion and deposition of coarse clastic sediments. A depositional age of ca. 470 Ma, obtained for the gravellites in the Lake Songkul area, is in agreement with the timing of deposition for lower Ordovician conglomerates elsewhere in the Northern Tien Shan, and corresponds to the main phase of the Andean type magmatism. The Songkultau adakites in association with surrounding ocean floor and island arc formations constitute a relic of a primitive Cambrian arc and represent a juvenile domain of substantial size identified so far within the predominantly crustal-derived terranes of Tien Shan. On a regional scale this primitive arc can be compared with juvenile Cambrian arcs of Kazakhstan, Gorny Altai and Mongolia.
The results are compared with the data of previous experiments on sand samples containing tetrahydrofuran (THF) and methane hydrate formed by the "excess gas" method.

The approach to the formation of sand samples containing methane hydrate of a non-cementing type is studied. The approach is to inject methane-rich water in the sample after formation of methane hydrate by the "excess gas" method. Laboratory research is carried out on a specialized setup that allows to form hydrate -bearing samples and to study their acoustic properties during the experiments.

It is shown that the studied approach allow to form samples containing methane hydrate of a non-cementing type.
A search for a heavy, CP-odd Higgs boson, A, decaying into a Z boson and a 125 GeV Higgs boson, h, with the ATLAS detector at the LHC is presented.

The search uses proton–proton collision data at a centre-of-mass energy of 8 TeV corresponding to an integrated luminosity of 20.3 fb−1. Decays of CP-even h bosons to ττ or bb pairs with the Z boson decaying to electron or muon pairs are considered, as well as h→bb decays with the Z boson decaying to neutrinos.

No evidence for the production of an A boson in these channels is found and the 95% confidence level upper limits derived for σ(gg→A)×BR(A→Zh)×BR(h→ff¯) are 0.098–0.013 pb for f=τ and 0.57–0.014 pb for f=b in a range of mA=220–1000GeV. The results are combined and interpreted in the context of two-Higgs-doublet models.
In 2021, the Sagai archaeological team of the Institute of Archeology and Ethnography SB RAS excavated four funerary and memorial complexes in the construction zone of the «Second track on the Chаrtykovsкy - Kamyshta stretch of the Krasnoyarsk railway». Mound 2 of the Sagayskaya protoka 2 burial ground contained 11 graves. Traces of diverse funeral rites were noted: intact corpses and partial burials of adults and infants in stone boxes and ground pits without stone structures. The grave inventory is poor - vessels and two iron objects (a knife and a buckle). Petroglyphic images were noted on the slabs of the boxes. Among them, there is a composition of the battle of two warriors, one of whom is on skis.

At the site of the “Single kurgan Sagayskaya protoka 5”, Tesinsky graves 1 and 3 were made in the stone ceiling of the Saragash grave. In grave 1, two adults were buried. A partial burial (grave 2) was found at some distance. The burials are tentatively dated to the range of the 1st century BC - 1st century AD. In addition to the burials, an excavation trench was established revealing cultural layer containing roughly made stone tools from river pebbles, fragments of animal bones and ceramic vessels with ornaments typical for the Tashtyk pottery.

In the areas associated with burial mound 6 (burial site Sagayskaya protoka 2) and burial mounds 1 and No. 2 (burial ground Sagayskaya protoka 8), 39 cult sites were recorded. By their construction, the sites can be classified into several groups: ground pits with animal bones and vessels under the stone work ceiling; ground pits with the remains of wooden posts; ground pits filled with rubble stones; slabs with the edge dug in the ground or tumbled down. The features of the ceramics make it possible to attribute the graves to the Tashtyk culture.
Inflorescences of wheat species, spikes, are characteristically unbranched and bear one sessile spikelet at a spike rachis node. Development of supernumerary spikelets (SSs) at rachis nodes or on the extended rachillas is abnormal. Various wheat morphotypes with altered spike morphology, associated with the development of SSs, present an important genetic resource for studies on genetic regulation of wheat inflorescence development.

Here we characterized diploid and tetraploid wheat lines of various non-standard spike morphotypes, which allowed for identification of a new mutant allele of the WHEAT FRIZZY PANICLE (WFZP) gene that determines spike branching in diploid wheat Ttiticum monococcum L. Moreover, we found that the development of SSs and spike branching in wheat T. durum Desf. was a result of a wfzp-A/TtBH-A1 mutation that originated from spontaneous hybridization with T. turgidum convar. сompositum (L.f.) Filat. Detailed characterization of the false-true ramification phenotype controlled by the recessive sham ramification 2 (shr2) gene in tetraploid wheat T. turgidum L. allowed us to suggest putative functions of the SHR2 gene that may be involved in the regulation of spikelet meristem fate and in specification of floret meristems. The results of a gene interaction test suggested that genes WFZP and SHR2 function independently in different processes during spikelet development, whereas another spike ramification gene(s) interact(s) with SHR2 and share(s) common functions.

SS mutants represent an important genetic tool for research on the development of the wheat spikelet and for identification of genes that control meristem activities. Further studies on different non-standard SS morphotypes and wheat lines with altered spike morphology will allow researchers to identify new genes that control meristem identity and determinacy, to elucidate the interaction between the genes, and to understand how these genes, acting in concert, regulate the development of the wheat spike.
Nuclear magnetic resonance (NMR) has become a universal method for biochemical and biomedical studies, including metabolomics, proteomics, and magnetic resonance imaging (MRI). By increasing the signal of selected molecules, the hyperpolarization of nuclear spin has expanded the reach of NMR and MRI even further (e.g. hyperpolarized solid‐state NMR and metabolic imaging in vivo).

Parahydrogen (pH 2 ) offers a fast and cost‐efficient way to achieve hyperpolarization, and the last decade has seen extensive advances, including the synthesis of new tracers, catalysts, and transfer methods.

The portfolio of hyperpolarized molecules now includes amino acids, which are of great interest for many applications. Here, we provide an overview of the current literature and developments in the hyperpolarization of amino acids and peptides.
Tip-clearance cavitation is one of the most aggressive forms of cavitation as it can cause surface erosion of hydraulic machinery elements and, as a result, their fatigue damage and disturb designed operating conditions. At present, the literature lacks for detailed experimental data on the inception and development of this type of cavitation at various flow conditions.

In the paper, a tip-leakage cavitation occurring in the clearance between an end face of a 2D hydrofoil (a scaled-down model of guide vanes (GV) of a Francis turbine) and a transparent wall of the test section was studied. The experiments were carried out for different cavitating regimes on the cavitation number and two attack angles of 3° and 9°, with the gap size (tip clearance width) varied in the range from 0.4 to 0.8 mm. In order to determine the cavitation inception conditions and investigate the dynamics of the tip-leakage cavitation, a high-speed visualization was applied. A modified PIV/PTV technique with a diverging laser beam instead of a laser light sheet was used to measure the mean velocity distributions within the gap.

It was shown that the cavitation pattern on the suction side of the GV model impacts the dynamics of the leakage flow in the gap but does not affect the sheet cavity formed close to the foil leading edge in the clearance as well as its size and dynamics. When the gap size is increased, the tip-leakage cavitation initiates at higher cavitation numbers or, in other words, conditions for the cavitation occurrence become more favorable.
Diamond crystallization in melts of europium salts (Eu2(C2O4)3·10H2O, Eu2(CO3)3·3H2O, EuCl3, EuF3, EuF2) at 7.8 GPa and in a temperature range of 1800–2000 °C was studied for the first time.

Diamond growth on seed crystals was realized at a temperature of 2000 °C. Spontaneous diamond nucleation at these parameters was observed only in an Eu oxalate melt. The maximum growth rate in the europium oxalate melt was 22.5 μm/h on the {100} faces and 12.5 μm/h on the {111} faces.

The diamond formation intensity in the tested systems was found to decrease in the following sequence: Eu2(C2O4)3·10H2O > Eu2(CO3)3·3H2O > EuF3 > EuF2 = EuCl3. Diamond crystallization occurred in the region of stable octahedral growth in melts of Eu3+ salts and in the region of cubo-octahedral growth in an EuF2 melt. The microrelief of faces was characterized by specific features, depending on the system composition and diamond growth rate. In parallel with diamond growth, the formation of metastable graphite in the form of independent crystals and inclusions in diamond was observed. From the spectroscopic characterization, it was found that diamonds synthesized from Eu oxalate contain relatively high concentrations of nitrogen (about 1000−1200 ppm) and show weak PL features due to inclusions of Eu-containing species.
The 3-D printers offered to the construction market can perform retained form layer-by-layer for subsequent filling with reinforced structural concrete and insulation.

The use of a single-stage polystyrene concrete with polystyrene granules bloated directly in the printer head

allows to form layer-by-layer the insulated wall for its entire width and reduce the erection operation to one technological conversion.
Aim : to study the association between hereditary/acquired thrombophilia and premature detachment of normally located placenta.

Materials and methods . The participating women were divided into 2 groups: the main group (n = 38) and the control group (n = 43). The main group included women, whose pregnancy or delivery was complicated by premature detachment of normally located placenta. The control group included women without previous obstetric or thrombotic complications, who gave birth to full-term live babies with an Apgar score of 8-10. The key coagulogram parameters, the presence of hyperhomocysteinemia, antiphospholipide syndrome, and polymorphisms of the hemostasis genes (by real-time polymerase chain reaction) were determined.

Results . A statistically significant increase in the concentration of fibrinogen and D-dimer was found in patients of the main group. In addition, allele A of the G1691A polymorphic locus of the factor V gene and allele 4G of the 5G/4G 675 polymorphic locus of the PAI-1 gene are risk factors of placental detachment development. Conclusion . Hereditary and acquired thrombophilia are significant factors in the development of premature detachment of the normally located placenta.
We have shown for the first time that simple annealing of silver–synthetic diamond mixtures in air or oxygen at 600–700 °C leads to selective growth of silver particles on the {100} facets of diamond, while no growth is observed on the {111} facets. Annealing at a temperature higher than 750 °C results in significant morphological changes in the heterostructure: silver particles grow not only on the cube but also on the octahedral facets.
Performance of flash radiography units implies a high spatial resolution. Thus, focused electron beams are utilized to produce bremsstrahlung x-ray at the solid target. Strong heating of the target up to 1-10 eV temperature and formation of dense plasma can lead to beam disruption. In case of multi-pulse operation mode of radiography unit the plasma produced by the first pulse can strongly affect subsequent pulses. In present work we utilize the model of Zeldovich and Raizer to estimate the electron density in the target plasma. The typical parameters of radiography units based on linear induction accelerators is considered. It is shown that electron density in plasma expanding after first pulse is high enough to affect subsequent electron beams.
Transgenic potato plants cv.Golubizna carrying the gene for extracellular ribonuclease ZRNaseII under the control of the strong constitutive 35S promoter of cauliflower mosaic virus

shows some resistance to P. infestans infection, manifested in delayed symptoms of infection

suggesting the involvement of S-like RNase in the system of a nonspecific response to pathogen damage
Family of “breathing crystals” is the polymer-chain complexes of Cu(hfac)2 with nitroxides. The polymer chains consist of one-, two- or three-spin clusters. The “breathing crystals” experience simultaneous magnetic and Jahn-Teller type structural phase transitions with change of total cluster spin and drastic change of bond lengths (ca. 10-12%). For the first time the intra-cluster magnetic couplings in ”breathing crystals” have been calculated both by band structure methods GGA + U and hybrid DFT (B3LYP and PBE0) for the isolated exchange clusters. The temperature dependence of the magnetic coupling constant was calculated for two polymer-chain compounds of the “breathing crystal” family - C21H19CuF12N4O6 with the chains containing two-spin clusters and C22H21CuF12N4O6 with the chains of alternating three-spin clusters and one-spin sites. It was found that adding a Hubbard-like parameter not only to the copper 3d electrons but also to the oxygen 2p electrons (GGA + Ud + Up approach) results in an improved description of exchange coupling in the “breathing crystal” compounds. At the same time treatment of the isolated clusters by a large basis hybrid DFT with high computational cost provides a similar quality fit of the experimental magneto-chemical data as that for the GGA + Ud + Up band structure calculation scheme. Our calculations also showed that in spite of the abrupt transformation of the magnetic coupling constant under the phase transition, the band gap in the “breathing crystals” remains about the same value with temperature decrease.
The paper presents the results of an analytical review of the study properties rocks and their saturating fluids by dielectric spectroscopy

as well as experimental studies of their complex dielectric constant and NMR characteristics

The connection of the complex dielectric constant of oil with its geochemical properties and NMR characteristics is established
The current limits for fourth generation quarks allows to expect their mass of the order of 500 GeV. In this mass region for quark–antiquark pair the additional Yukawa-type attraction due to Higgs mechanism is expected to emerge.

In the key of recent works on significance of color-octet channel for production of color-singlet state of fourth generation [Formula: see text] we calculated the binding energies for both octet and singlet states.

This Higgs-induced attraction greatly exceeds strong interaction between quarks and leads to the formation of bound states in both color-octet S (8) and singlet S (1) states. Such an attraction localizes quarks in extremely small area. Hence, color-octet pair of fourth generation quarks can form the 'nucleus' and, together with color neutralizing light particle that is captured by strong interaction in orbit around the nucleus, create particle with structure similar to Deuterium.
(1)H NMR signal amplification by reversible exchange (SABRE) was observed for pyridine and pyridine-d5 at 9.4 T, a field that is orders of magnitude higher than what is typically utilized to achieve the conventional low-field SABRE effect. In addition to emissive peaks for the hydrogen spins at the ortho positions of the pyridine substrate (both free and bound to the metal center), absorptive signals are observed from hyperpolarized orthohydrogen and Ir-complex dihydride. Real-time kinetics studies show that the polarization build-up rates for these three species are in close agreement with their respective (1)H T1 relaxation rates at 9.4 T. The results suggest that the mechanism of the substrate polarization involves cross-relaxation with hyperpolarized species in a manner similar to the spin-polarization induced nuclear Overhauser effect. Experiments utilizing pyridine-d5 as the substrate exhibited larger enhancements as well as partial H/D exchange for the hydrogen atom in the ortho position of pyridine and concomitant formation of HD molecules. While the mechanism of polarization enhancement does not explicitly require chemical exchange of hydrogen atoms of parahydrogen and the substrate, the partial chemical modification of the substrate via hydrogen exchange means that SABRE under these conditions cannot rigorously be referred to as a non-hydrogenative parahydrogen induced polarization process.
A new type of coronavirus—severe acute respiratory syndrome coronavirus 2 causing coronavirus disease 2019 (COVID-19) emerged in 2019. On March 11, 2020, World Health Organization (WHO) Director-General declared that the outbreak can be characterized as a pandemic. According to the WHO, on March 18, 2020, the number of laboratory-confirmed cases of COVID-19 was over 200 thousands worldwide. Both influenza viruses and coronaviruses are respiratory pathogens with similar routes of infection and related pathogenesis. As the emergence of COVID-19 coincides in time with epidemics of seasonal influenza in the Northern hemisphere, it is possible to assess efforts of health services for controlling coronavirus using the dynamics of influenza epidemics in certain countries.

This study demonstrates data analysis of several regions of the Northern hemisphere. Specifically, in China, the highest rate of the influenza epidemic was registered in the second week of 2020. On January 25, anti-epidemic measures were tightened because of COVID-19. WHO weekly updates data on the confirmed influenza cases in each country (FluNet-CHARTS). Data analysis includes China, South-West Europe, and North America regions with their respective influenza case statistics and timeline comparisons.

China has coped with the COVID-19 pandemic simultaneously with the seasonal influenza epidemic. WHO data demonstrate the impact of anti-epidemic measures on the influenza epidemic of 2020. However, those measures differently affected COVID-19 in the three aforementioned regions. The epidemiological response in China stopped the COVID-19 epidemic spreading, while in Italy and the USA the result is unsatisfactory. It is believed that in future new viruses pathogenic for humans will emerge due to the presence of an unlimited natural reservoir of animal viruses. Anti-epidemic measures can stop the seasonal influenza epidemic and possibly even pandemic caused by highly pathogenic influenza viruses provided that those measures are timely efforts of all or at least of the majority of countries worldwide. A concerted strategy should be elaborated for performing public health measures at the emergence of new respiratory viruses pathogenic for humans.
During the field works in the summer of 2018, in the Lena River delta on the Sardakh-Sisse Island, prints of leaf flora belonging to species of broad and small-leaved deciduous trees were found. The prints found by us are confined to the layer of ferruginous sandstones at the base of the section. The following taxa established: Platanus sp., Alnus sp., Fagus sp., Salix sp. In addition, a fossilized fruit belonging to Magnolia sp. The findings allow to conclude that there are mixed forests in the early-middle Miocene in this region, consisting of coniferous, small- and broad-leaved deciduous species of trees and shrubs.