Hengzongshu/ArticleAgent huggingface dataset:
Low-temperature interaction of [1,2,5]thiadiazolo[3,4-c][1,2,5]thiadiazole (1) with MoMes2 (Mes = mesitylene/1,3,5-trimethylbenzene) in tetrahydrofuran gave the heterospin (S1 = S2 = 1/2) radical-ion salt [MoMes2]+[1]− (2) whose structure was confirmed by single-crystal X-ray diffraction (XRD). The structure revealed alternating layers of the cations and anions with the Mes ligands perpendicular, and the anions tilted by 45°, to the layer plane. At 300 K the effective magnetic moment of 2 is equal to 2.40 μB (theoretically expected 2.45 μB) and monotonically decreases with lowering of the temperature. In the temperature range 2–300 K, the molar magnetic susceptibility of 2 is well-described by the Curie–Weiss law with parameters C and θ equal to 0.78 cm3 K mol–1 and −31.2 K, respectively. Overall, the magnetic behavior of 2 is similar to that of [CrTol2]+[1]− and [CrCp*2]+[1]−, i.e., changing the cation [MAr2]+ 3d atom M = Cr (Z = 24) with weak spin–orbit coupling (SOC) to a 4d atom M = Mo (Z = 42) with stronger SOC does not affect macroscopic magnetic properties of the salts. For the XRD structure of salt 2, parameters of the Heisenberg spin-Hamiltonian were calculated using the broken-symmetry DFT and CASSCF approaches, and the complex 3D magnetic structure with both the ferromagnetic (FM) and antiferromagnetic (AF) exchange interactions was revealed with the latter as dominating. Salt 2 is thermally unstable and slowly loses the Mes ligands upon storage at ambient temperature. Under the same reaction conditions, interaction of 1 with MoTol2 (Tol = toluene) proceeded with partial loss of the Tol ligands to afford diamagnetic product.
We report a measurement of the branching fraction and final-state asymmetry for the $\bar{B}^{0}\to K^{0}_{S}K^{\mp}\pi^{\pm}$ decays. The analysis is based on a data sample of 711 $\rm{fb}^{-1}$ collected at the $\Upsilon(4S)$ resonance with the Belle detector at the KEKB asymmetric-energy $e^{+}e^{-}$ collider. We obtain a branching fraction of $(3.60\pm0.33\pm0.15)\times10^{-6}$ and a final-state asymmetry of $(-8.5\pm8.9\pm0.2)\%$, where the first uncertainties are statistical and the second are systematic. Hints of peaking structures are seen in the differential branching fractions measured as functions of Dalitz variables.
Aim. To evaluate the efficiency of cardioprotective therapy using intravenous metoprolol in combination with a high dose of atorvastatin in the prevention of myocardial remodeling (MR) and heart failure (HF) in patients with acute ST-segment elevation myocardial infarction (STEMI). Material AND methods. A prospective study included 100 STEMI patients who underwent primary percutaneous intervention (PCI). Depending on the regimens of drug cardioprotection, three groups of patients were formed: the first (2014–2015) — 34 patients who received 80 mg atorvastatin as a part of the basic therapy on the first day of STEMI, then 20–40 mg/day for 30 days. The second group (2017–2018) — 34 patients who received atorvastatin 80 mg/day for a month from the onset of STEMI. The third group (2018–2019) — 32 patients who received intravenous metoprolol tartrate (5–15 mg) and atorvastatin 80 mg/day before PCI for a month from the onset of STEMI. On days 1 and 2 of STEMI and one month later, patients were assessed for serum levels of cardiac biomarkers; on the 1st, 7th days and one month later, echocardiographic studies (EchoCG) were performed. At the end of the observation, clinical and imaging outcomes (MR and HF) were assessed, which were compared with the dynamics of biomarkers between the groups of patients. Results. The combined use of atorvastatin 80 mg/day for a month from the onset of STEMI and a single intravenous injection of metoprolol tartrate (5–15 mg) in the acute phase of STEMI before PCI showed the most significant effects in the prevention of the development of structural and functional myocardial disorders and clinically severe heart failure, and also caused the minimal serum activity of cardiomarkers in the third group of patients in comparison with the first and second groups of patients without this drug combination. Also, correlations between biomarkers and echocardiography indicators were established in the third group of patients who received cardioprotective therapy. Conclusion. The combined use of high-dose atorvastatin for a month with a single intravenous injection of metoprolol tartrate in acute STEMI before PCI prevents the formation of MR and clinically significant HF in the post-infarction period. Comprehensive dynamic assessment of cardiac biomarkers and echocardiography parameters within a month after post-STEMI is a highly informative tools for monitoring the efficiency of cardioprotective therapy.
Human prefrontal cortex (PFC) is associated with broad individual variabilities in functions linked to personality, social behaviors, and cognitive functions. The phenotype variabilities associated with brain functions can be caused by genetic or epigenetic factors. The interactions between these factors in human subjects is, as of yet, poorly understood. The heterogeneity of cerebral tissue, consisting of neuronal and nonneuronal cells, complicates the comparative analysis of gene activities in brain specimens. To approach the underlying neurogenomic determinants, we performed a deep analysis of open chromatin–associated histone methylation in PFC neurons sorted from multiple human individuals in conjunction with whole-genome and transcriptome sequencing. Integrative analyses produced novel unannotated neuronal genes and revealed individual-specific chromatin "blueprints" of neurons that, in part, relate to genetic background. Surprisingly, we observed gender-dependent epigenetic signals, implying that gender may contribute to the chromatin variabilities in neurons. Finally, we found epigenetic, allele-specific activation of the testis-specific gene nucleoporin 210 like (NUP210L) in brain in some individuals, which we link to a genetic variant occurring in <3% of the human population. Recently, the NUP210L locus has been associated with intelligence and mathematics ability. Our findings highlight the significance of epigenetic-genetic footprinting for exploring neurologic function in a subject-specific manner.—Gusev, F. E., Reshetov, D. A., Mitchell, A. C., Andreeva, T. V., Dincer, A., Grigorenko, A. P., Fedonin, G., Halene, T., Aliseychik, M., Goltsov, A. Y., Solovyev, V., Brizgalov, L., Filippova, E., Weng, Z., Akbarian, S., Rogaev, E. I. Epigenetic-genetic chromatin footprinting identifies novel and subject-specific genes active in prefrontal cortex neurons. FASEB J. 33, 8161–8173 (2019). www.fasebj.org
 НАУКИ О ЗЕМЛЕ (25.00.00)  УДК 551.89(571.1)ДИСКУССИоННЫе ПРоБЛеМЫ ПаЛеогеогРаФИИ ПЛеЙСТоЦеНа ЛеДНИКоВЫХ оБЛаСТеЙ ЗаПаДНоЙ СИБИРИ 1,2 Зыкин В.С., 1 Зыкина В.С. 1 ФГБУН «Институт геологии и минералогии СО РАН им В.С.Соболева», Новосибирск, e-mail: zykin
The torque of the rotational resistance in the Ku-Etta multi-cylinder system rotating in the direction towards each other is measured.

The experiments were carried out for three values of the kinematic viscosity of the working fluid that fills the multicylinder system: water at a temperature of 24 °C (viscosity 0.9 cSt), an aqueous solution of glycerol at 20 °C and 41 °C (2.5 cSt and 5.2 cSt).

An attempt is made to investigate the features of a viscous flow in the multicolor Couette flow system from the analysis of the energy spectra of the moment of resistance to rotation of cylinders.
Experimental evidence of the plasma flow suppression by the helical magnetic mirror is presented. Reported experiments were done during the first plasma campaign in the SMOLA helical mirror device at self-consistent floating potentials of all in-vessel electrodes and at minimal magnetic fields suitable for confinement regime.

The experimental results are consistent with two main theory predictions for the helical mirror confinement: a reduction of the axial plasma flow and the inward particle pinch.

The helical mirror technology can dramatically improve fusion reactor prospects of open magnetic configurations.
The interference fragmentation function translates the fragmentation of a quark with a transverse projection of the spin into an azimuthal asymmetry of two final-state hadrons. In e+e- annihilation the product of two interference fragmentation functions is measured. We report nonzero asymmetries for pairs of charge-ordered pi+pi- pairs, which indicate a significant interference fragmentation function in this channel. The results are obtained from a 672 fb-1 data sample that contains 711 \times 106 pi+pi- pairs and was collected at and near the ?(4S) resonance, with the Belle detector at the KEKB asymmetric-energy e+e- collider.
To better understand how a nervous system controls the movements of an organism

we have created a three-dimensional computational biomechanical model of the Caenorhabditis elegans body based on real anatomical structure. The body model is created with a particle system-based simulation engine known as Sibernetic, which implements the smoothed particle-hydrodynamics algorithm. The model includes an elastic body-wall cuticle subject to hydrostatic pressure. This cuticle is then driven by body-wall muscle cells that contract and relax, whose positions and shape are mapped from C. elegans anatomy, and determined from light microscopy and electron micrograph data.

We show that by using different muscle activation patterns, this model is capable of producing C. elegans-like behaviours, including crawling and swimming locomotion in environments with different viscosities, while fitting multiple additional known biomechanical properties of the animal. This article is part of a discussion meeting issue 'Connectome to behaviour: modelling C. elegans at cellular resolution'.
KEDR detector has the tagging system (TS) to study the gamma-gamma processes. To determine the two-photon invariant mass, the energies of the scattered at small angles electrons and positrons are measured by the magnetic spectrometer embedded into the lattice of the VEPP-4M collider.

The Compton backscattering of laser radiation on the electron/positron beam is used for the accurate energy scale and resolution calibration of the tagging system.

The energy resolution (scattered electron/positron energy resolution divided by the beam energy) of this spectrometer varies from 0.6% to 0.03% depending on the electron/positron energy. The report covers the design, recent results and current status of the KEDR TS calibration system.
Hydrogels are biocompatible matrices for local delivery of nucleic acids; however, functional dopants are required to provide efficient delivery into cells. In particular, dendrimers, known as robust nucleic acid carriers, can be used as dopants. Herein, we report the first example of impregnating neutral hydrogels with siRNA–dendrimer complexes. The surface chemistry of dendrimers allows adjusting the release rate of siRNA-containing complexes. This methodology can bring new materials for biomedical applications.
For the incompressible Navier-Stokes equations in the 3D half space

we show the existence of forward self-similar solutions

for arbitrarily large self-similar initial data
Visualizing hypergraphs, systems of subsets of some universe, has continuously attracted research interest in the last decades. We study a natural kind of hypergraph visualization called subdivision drawings. Dinkla et al. [Comput. Graph. Forum '12] claimed that only few hypergraphs have a subdivision drawing. However, this statement seems to be based on the assumption (also used in previous work) that the input hypergraph does not contain twins, pairs of vertices which are in precisely the same hyperedges (subsets of the universe).

We show that such vertices may be necessary for a hypergraph to admit a subdivision drawing. As a counterpart, we show that the number of such 'necessary twins' is upper-bounded by a function of the number m of hyperedges and a further parameter r of the desired drawing related to its number of layers.

This leads to a linear-time algorithm for determining such subdivision drawings if m and r are constant; in other words, the problem is linear-time fixed-parameter tractable with respect to the parameters m and r.
The correct deployment of genetic programs for development and differentiation relies on finely coordinated regulation of specific gene sets. Genomic regulatory elements play an exceptional role in this process. There are few types of gene regulatory elements, including promoters, enhancers, insulators and silencers. Alterations of gene regulatory elements may cause various pathologies, including cancer, congenital disorders and autoimmune diseases. The development of high-throughput genomic assays has made it possible to significantly accelerate the accumulation of information about the characteristic epigenetic properties of regulatory elements.

In combination with high-throughput studies focused on the genome-wide distribution of epigenetic marks, regulatory proteins and the spatial structure of chromatin, this significantly expands the understanding of the principles of epigenetic regulation of genes and allows potential regulatory elements to be searched for in silico. However, common experimental approaches used to study the local characteristics of chromatin have a number of technical limitations that may reduce the reliability of computational identification of genomic regulatory sequences. Taking into account the variability of the functions of epigenetic determinants and complex multicomponent regulation of genomic elements activity, their functional verification is often required. A plethora of methods have been developed to study the functional role of regulatory elements on the genome scale. Common experimental approaches for in silico identification of regulatory elements and their inherent technical limitations will be described. The present review is focused on original high-throughput methods of enhancer activity reporter analysis that are currently used to validate predicted regulatory elements and to perform de novo searches.

The methods described allow assessing the functional role of the nucleotide sequence of a regulatory element, to determine its exact boundaries and to assess the influence of the local state of chromatin on the activity of enhancers and gene expression. These approaches have contributed substantially to the understanding of the fundamental principles of gene regulation.
It has long been known that defects in the structure of the mitochondrial genome can cause various neuromuscular and neurodegenerative diseases. Nevertheless, at present there is no effective method for treating mitochondrial diseases. The major problem with the treatment of such diseases is associated with mitochondrial DNA (mtDNA) heteroplasmy. It means that due to a high copy number of the mitochondrial genome, mutant copies of mtDNA coexist with wild-type molecules in the same organelle. The clinical symptoms of mitochondrial diseases and the degree of their manifestation directly depend on the number of mutant mtDNA molecules in the cell. The possible way to reduce adverse effects of the mutation is by shifting the level of heteroplasmy towards the wild-type mtDNA molecules. Using this idea, several gene therapeutic approaches based on TALE and ZF nucleases have been developed for this purpose. However, the construction of protein domains of such systems is rather long and laborious process. Meanwhile, the CRISPR/Cas9 system is fundamentally different from protein systems in that it is easy to use, highly efficiency and has a different mechanism of action. All the characteristics and capabilities of the CRISPR/Cas9 system make it a promising tool in mitochondrial genetic engineering. In this article, we demonstrate for the first time that the modification of gRNA by integration of specific mitochondrial import determinants in the gRNA scaffold does not affect the activity of the gRNA/Cas9 complex in vitro.
There are various methods for calculating rarefied gas flows, in particular, statistical methods and deterministic methods based on the finite-difference solutions of the Boltzmann nonlinear kinetic equation and on the solutions of model kinetic equations. There is no universal method; each has its disadvantages in terms of efficiency or accuracy. The choice of the method depends on the problem to be solved and on parameters of calculated flows.

The paper provides the results of the calculations performed by the authors with the help of the Direct Simulation Monte Carlo method and finite-difference methods of solving the Boltzmann equation and model kinetic equations.

Based on this comparison, conclusions are made on selecting a particular method for flow simulations in various ranges of flow parameters.
The paper presents the results of experimental studies on the synthesis of carbide Fe7C3 at 5,5 GPa. It was found that carbide Fe3C and several polytypes of carbide Fe7C3 are formed together with diamond when the system is cooled. It is believed that Fe7C3 carbide may be a component of the Earth’s inner core. The obtained results indicate that carbide Fe7C3 in the form of polytypic modifications under natural conditions could be formed at relatively low pressures at the stage of differentiation of the Earth.
Invasive earthworms often have huge ecological impact, especially when they colonize previously earthworm-free zones. Kamchatka is a remote and weakly populated region whose invertebrate fauna is insufficiently studied.

We investigated earthworm diversity in several natural and anthropogenic habitats of Kamchatka by morphological analysis and barcoding using the mitochondrial cox1 gene.

Our results suggest it might be possible that the whole earthworm fauna of Kamchatka is of invasive origin, which makes it a good model for studying the effects of earthworm invasions into previously earthworm-free regions.
A new scheme of wave fields modelling from point sources in a homogeneous isotropic elastic medium is developed.

To set the source the developed technique is based on the use of additional components of the finite-difference grid and the use of all the stress tensor components in the nodes.

The results of the scheme employment in the two-dimensional homogeneous isotropic medium are presented; they demonstrate the numerical solutions' improvement for the source being under consideration in the article.
The work is devoted to the experimental study of the viscosity and rheology of nanofluids based on ethylene glycol with single-walled nanotubes. Polyvinylpyrrolidone is used as a dispersant. The weight concentration of nanotubes ranged from 0.01 to 1%. In all cases, the weight concentration of the dispersant was controlled. The measurements were carried out in the temperature range from 20 to 40°C. It was shown that at all weight concentrations the solution of ethylene glycol and dispersant is a Newtonian liquid, and at a maximum concentration (4%) the viscosity of the solution is two and a half times higher than the viscosity of ethylene glycol. At the same time, all the studied nanofluids turned out to be non-Newtonian and have a pseudoplastic character. It was established that ultrasonic treatment of nanofluids leads to partial degradation of the dispersant and a decrease the base fluid viscosity. The degree of this degradation increases with increasing ultrasonic processing power. With the considered low weight concentrations of nanotubes, the dependence of the viscosity coefficient of the nanofluid on temperature is determined by the corresponding dependence of the base fluid.
Recent climatic disasters (dzudzs) and uncontrolled massive breeding endangered valuable ge-netic resources of Mongolian goats labored by five thousand years of evolution in extremes of Al-tai mountains and Gobi deserts. Meanwhile, Mongolian goats has never been characterized by genomic data. We used Illumina Goat SNP50 to investigate phylogenic relationships and genetic risks in 5 local Mongolian populations: Erchim (N=37) from geographically remote Darkhat Valley in the northern mountains, Ulgii Red (N=35) and Dorgon (N=28) from the western mountain region, Buural (N=34) from the western steppe and mountain areas and Gobi Gurvan Saikhan (N=33) from semi-arid steppe area in the South Gobi region. ROH analyses, estimated populations sizes and Fst values showed South Gobi and Darkhat Valley goats to be of a high risk of inbreed-ing that however appear to be of distinct origin (artificial selection vs geographical isolation). In-dices of genetic differentiations between Mongolian goats were relatively low compared to Euro-pean breeds. Meanwhile, we suggest direct comparison is not fully appropriate given that Mongo-lian breeds were not subjected to intense selection. Darkhat Valley goats were clearly differentiat-ed from other Mongolian breeds according to various types of analyses. Phylogenetic relationships within XXX breeds of the rest of the world plotted Mongolian goats between some Russian (Altai, Orenburg) and Chinese populations (Nanjiang and Qinggeli). Thus, present study (i) highlights demographic history Mongolian goats and (ii)provides unified SNP-data called for support deci-sions in conservational genetics. Finally, our work (iii) raises a question of how exactly these data should be compared to make objective choices.
The aim was to investigate behavioral reactions and event-related potential (ERP) responses in healthy participants under conditions of personalized attribution of emotional appraisal vocabulary to one-self or to other people. One hundred and fifty emotionally neutral, positive and negative words describing people’s traits were used. Subjects were asked to attribute each word to four types of people: one-self, loved, unpleasant and neutral person. The reaction time during adjectives attribution to one-self and a loved person was shorter than during adjectives attribution to neutral and unpleasant people. Self-related adjectives induced higher amplitudes of the N400 ERP peak in the medial cortical areas in comparison with adjectives related to other people. The amplitude of P300 and P600 depended on the emotional valence of assessments, but not on the personalized attribution. The interaction between the attribution effect and the effect of emotional valence of assessments was observed for the N400 peak in the left temporal area. The maximal amplitude of N400 was revealed under self-attributing of emotionally positive adjectives. Our results supported the hypothesis that the emotional valence of assessments and the processing of information about one-self or others were related to the brain processes that differ from each other in a cortical localization or time dynamics.
This paper presents the results of an experimental study of the evaporation rate of suspended droplets pure water and a binary ethanol-water solution under conditions of forced convection (Re > 100). The results are compared with experimental data of other authors on evaporation of pure liquids. The evaporation rates of pure water droplets at different velocities of the flowing stream are measured. The dependence of the evaporation rate on the concentration of the less-volatile component is studied and the degree of the speed of the oncoming flow on the evaporation efficiency is estimated. A generalization of the experimental data shows that with an increase in the velocity of the incoming flow, the rate of evaporation slightly increases, but the change in the parameter A is not linear, and with an increase in the velocity, the rate of its increase noticeably decreases. Note that with the evaporation of ethanol-water solutions, the number of factors influencing the rate of evaporation increases, which complicates the generalization of data and the conduct of computational studies.