Hengzongshu/ArticleAgent huggingface dataset:
Assisted reproductive technologies (ART) increasingly occupy the study of human reproduction. In addition, in developed countries they contribute to breeding of more than 50 % of cattle. In the management of collections of genetic lines of laboratory animals, these technologies are obligatory components of cryopreservation and rederivation. ART procedures include the development of early embryos outside the mother's body and the high probability of incomplete synchronization of the physiological state of the surrogate mother and transplanted embryos. Since all this occurs at the stage of the highest susceptibility of embryos to epigenetic reprogramming, the full cycle of ART and its individual components can lead to stable phenotypic changes in the offspring. Their reality is confirmed by studies of the morphological and functional characteristics of sexually mature offspring of CD1 outbred mice, obtained using different variants of early embryo transplantation. Comparative studies of body mass and body composition, basal glucose level and response to glucose load (glucose-tolerance test - GTT) have been done on sexually mature males and females. Animals were separated in 4 groups according to the variant of embryo transplantation: group (control) - natural mating; group (2cl-bl) - incubation of 2-cell up to blastocysts; group (2cl-2cl) - removal and transplantation of the 2-cell embryo without incubation; group (Bl-bl) removal and transplantation of the blastocysts without incubation. All embryos were transplanted to recipient females of the same line. It was found that sexually mature offspring obtained with all variants of transplantations had a higher relative fat content and, correspondingly, lower lean mass compared to the control. This effect was more pronounced in females than in males. Unlike body compositions, embryo transplantations had a greater effect on basal glucose concentration and GTT in males than in females. In this case, the offspring of the 2cl-2cl and 2cl-bl groups were characterized by a higher tolerance to glucose load (GTT) compared with the control and the Bl-bl group. Stable deviations of body compositions and glucose homeostasis indices detected in experimental groups of progenies indicate the phenotypic significance of the embryo transplantations per se.
The crystal structure of the mineral uytenbogaardtite, a rare silver-gold sulfide, was solved using intensity data collected for a crystal from the type locality, the Comstock lode, Storey County, Nevada (USA). The study revealed that the structure is trigonal, space group R3̄c, with cell parameters a = 13.6952(5), c = 17.0912(8) Å and V = 2776.1(2) Å 3 . The refinement of an anisotropic model led to an R index of 0.0140 for 1099 independent reflections. The structure consists of a sub-lattice of sulfur atoms forming a distorted body-centred cubic arrangement. The structure contains distinct tri-atomic linear groups (S–Au–S) and Ag atoms bonded to four S atoms (from four different linear groups) in a distorted tetrahedral arrangement. On the basis of information gained from this characterization, uytenbogaardtite is here definitively proved to be structurally different from petzite, Ag 3 AuTe 2 and fischesserite, Ag 3 AuSe 2 . By use of high-quality single-crystal diffraction data, the symmetry of the mineral was found to be trigonal, and not tetragonal as erroneously supposed. A revaluation of the powder diffraction data listed in the scientific literature for uytenbogaardtite according to the structural results obtained here leads to an excellent agreement. Crystal-chemical features of uytenbogaardtite, Au2S, petrovskaite AgAuS, uytenbogaardtite–fischesserite series Ag3Au(S 2–x Se x ) and acanthite–naummanite series Ag 2 (S 1–x Se x ) are compared.
The evolution of the artificial wave packet in swept-wing supersonic boundary layer was experimentally studied at M = 2.

The localized disturbances were generated by a pulse glow discharge. Measurements were provided by a hot-wire anemometer. The spatial structure of the wave packet was studied.

It was found that the wave packet has an asymmetric shape. In addition, the velocity of the propagation downstream of the wave packet was estimated.
The article examines the representation of chaos motif, which is the most general one among archetypal motifs, in A. P. Chekhov’s The Murder (1895). Chaos motif arranges the plot structure, being represented in its variants, such as misbelief and nature and space disorder. In The Murder, the central event is determined by mental disorders of characters. Each of them stands on their unique religious outlook. Matvei Terekhov teaches his cousin Yakov to accept the fundamental rules of Church, but he is not always true to his beliefs, too. The true reason of murder is hate towards each other, including Yakov’s sister Aglaya – it is a hate crime, which represents total moral destruction. Matvei’s death awakens new feelings in Yakov’s perception. Now he understands what suffering is. As he looks at prisoners, he gains his faith. All he wants to ask is why it was so hard to reveal the truth. Besides chaotic existence problems, three other types of archetypical motifs are realized. These are the following: natural chaos, spatial chaos, and unsafe home. Nature stands between winter and spring, and it is time for spring to arrive. This causes disorder all around, and makes Matvei feel anxious and frustrated. Space semantics also explicate chaos, especially in the final scene, in which Yakov tries to extrapolate his actual Sakhalin experience on his previous life. The narrative is based on spatial concepts here. And, finally, the symbol of home loses its meaning of safety in the semantic structure of the story. It turns out to be somebody’s else but not the family’s. And it causes panic fears among its habitants. Fear is one of basic reflexes on chaos extension. We conclude that the archetypal motif of chaos connects the plot levels, such as the murder event, lack of faith in the main characters, nature, space, and disorder at home. Thus, it is proven to be the motif core of the story.
Multidisciplinary studies using geomorphological, geoarchaeological, and geochronological approaches indicate contrasting environmental changes in southeastern Altai beginning from the Late Pleistocene. Twenty-eight new radiocarbon dates from the subaerial complex overlying Late Neopleistocene sediments in the high-altitude Kurai and Chuya basins confi rm the degradation of a single ice-dammed reservoir in that area during the Early Holocene. In the fi rst half of the Holocene, those basins were fi lled with isolated lakes. At the Baratal River mouth in the western Kurai basin, a reservoir with a water level of at least 1480 m a.s.l. emerged ca 10–6.5 ka BP, whereas in the Chuya depression, numerous residual lakes had existed at least 8 ka BP. Landslide- and morainedammed lakes between the depressions in the Chuya valley had existed until 7–3 ka BP, when they drained away. The preservation state of in situ archaeological sites, their cultural affi liation, and their location within the depressions and along the main Chuya valley attest to spatial and temporal changes of the hydrological system. This evolution occurred in the second half of the Holocene and did not entail major consequences for humans.
The search for markers of predisposition to atherosclerosis development is very important for early identification of individuals with a high risk of cardiovascular disease.

This cross-sectional, population-based study was conducted in 468 subjects from the Novosibirsk region. It was shown that the mean (carotid intima-media thickness) cIMT correlated with the following mtDNA mutations: m.15059G>A (r = 0.159, p = 0.001), m.12315G>A (r = 0.119; p = 0.011), m.5178C>A (r = 0.114, p = 0.014), and m.3256C>T (r = 0.130, p = 0.011); a negative correlation with mtDNA mutations m.14846G>A (r = −0.111, p = 0.042) and m.13513G>A (r = −0.133, p = 0.004) was observed. In the linear regression analysis, the addition of the set of mtDNA mutations to the conventional cardiovascular risk factors increased the ability to predict the cIMT variability from 17 to 27%. Multi-step linear regression analysis revealed the most important predictors of mean cIMT variability: age, systolic blood pressure, blood levels of total cholesterol, LDL and triglycerides, as well as the mtDNA mutations m.13513G>A, m.15059G>A, m.12315G>A, and m.3256C>T.

Thus, a high predictive value of mtDNA mutations for cIMT variability was demonstrated. The association of mutation m.13513G>A and m.14846G>A with a low value of cIMT, demonstrated in several studies, represents a potential for the development of anti-atherosclerotic gene therapy.
The study is devoted to preliminary investigation Russian-language version of the questionnaire "Revised Parental Emotion Regulation Inventory" psychometric properties. The sample consists of 211 parents raising 1 child - 4 children, with 184 mothers (M=36,5) and 27 fathers (M=36,9). All five scales and total scale have good consistency (Cronbach's alpha from 0.78 and 0.84). Moderate retest reliability (one month interval) is shown (r = 0.75 to r = 0.61), which can indicate measurement of more situational characteristics of parental emotions regulation. Construct validity of the questionnaire was evaluated by means of correlations with scales that theoretically are connected with the emotional regulation in parents. It is generally acceptable. A higher levels of cognitive reassessment of unwanted child-related situations and suppression of thoughts about such situations were observed in parents raising children with autism spectrum disorders than in parents of neurotypical children. This indicates the criterion validity of the inventory. The results of the confirmatory factor analysis show incomplete compliance of the four-factor model with empirical data.
In this study, we demonstrated experimentally and theoretically that oxygen vacancies are responsible for the charge transport in HfO$_2$. Basing on the model of phonon-assisted tunneling between traps, and assuming that the electron traps are oxygen vacancies, good quantitative agreement between the experimental and theoretical data of current-voltage characteristics were achieved. The thermal trap energy of 1.25 eV in HfO$_2$ was determined based on the charge transport experiments.
TRANSFAC is a database on transcription factors, their genomic binding sites and DNA-binding profiles.

In addition to being updated and extended by new features, it has been complemented now by a series of additional database modules. among them, modules which provide data about signal transduction pathways (TRANSPATH) or about cell types/organs/developmental stages (CYTOMER) are available as well as an updated version of the previously described compel database.

the databases are available on the www at http://transfac.gbf.de/
Fragile X syndrome is the most common cause of inherited intellectual disability in humans. It is a result of CGG repeat expansion in the 5′ untranslated region (5′ UTR) of the FMR1 gene. This gene encodes the FMRP protein that is involved in neuronal development. Repeat expansion leads to heterochromatinization of the promoter, gene silencing, and the subsequent absence of FMRP. To date, there is no specific therapy for the syndrome. All treatments in clinic practice provide symptomatic therapy. The development of drug therapy for Fragile X syndrome treatment is connected with the search for inhibitors of enzymes that are responsible for heterochromatinization. Here, we report a weak transcriptional activity of the FMR1 gene and the absence of FMRP protein after Fragile X syndrome cell lines treatment with two FDA approved inhibitors of histone deacetylases, romidepsin and vorinostat. We demonstrate that romidepsin, an inhibitor of class I histone deacetylases, does not activate FMR1 expression in patient cell cultures, whereas vorinostat, an inhibitor of classes I and II histone deacetylases, activates a low level of FMR1 expression in some patient cell lines.
Biologically active substances are in the focus of pharmaceutical and chemical research. Serotonin, one of the most common neurotransmitters, is widely studied in relation to its effect on humans from cellular to neurological levels. Although serotonin plays a key role in some biological processes, its chemistry and crystallography are not sufficiently understood.

The aim of the present study was to crystallize serotonin adipate and creatinine sulfate monohydrate, determine their crystal structures, and analyze them in a comparison with other previously known serotonin crystal structures. Special attention was paid to the interrelation between the molecular conformation and crystalline environment. This issue was addressed using crystallographic and computational chemistry (DFT-D, MD) approaches.

In our research was shown that the crystal structure of the creatinine sulfate complex significantly differs from what was previously determined. The conformation of serotonin in the new structure differs from serotonin conformations in all other known complexes, as well as from the most stable conformation, predicted by the adiabatic conformational analysis using quantum chemical calculations (DFT, MP) in different phases. This work has explicitly shown the influence of different interactions on serotonin molecular conformation in the crystalline state, described from a crystallographic and theoretical point of view. It has been previously demonstrated that salt formation in the presence of different anions produces variation in pharmacological, therapeutic and physic-chemical properties. This study has shown that alterations of the anion affects the molecular geometry of the bioactive substance and invite further investigation to rationalize the geometry changes.
The main advantage of extracellular vesicles (EVs) as a drug carrier system is their low immunogenicity and internalization by mammalian cells. EVs are often considered a cell-specific delivery system, but the production of preparative amounts of EVs for therapeutic applications is challenging due to their laborious isolation and purification procedures. Alternatively, mimetic vesicles prepared from the cellular plasma membrane can be used in the same way as natural EVs.

For example, a cytoskeleton-destabilizing agent, such as cytochalasin B, allows the preparation of membrane vesicles by a series of centrifugations. Here, we prepared cytochalasin-B-inducible nanovesicles (CINVs) of various cellular origins and studied their tropism in different mammalian cells.

We observed that CINVs derived from human endometrial mesenchymal stem cells exhibited an enhanced affinity to epithelial cancer cells compared to myeloid, lymphoid or neuroblastoma cancer cells. The dendritic cell-derived CINVs were taken up by all studied cell lines with a similar efficiency that differed from the behavior of DC-derived EVs. The ability of cancer cells to internalize CINVs was mainly determined by the properties of recipient cells, and the cellular origin of CINVs was less important. In addition, receptor-mediated interactions were shown to be necessary for the efficient uptake of CINVs. We found that CINVs, derived from late apoptotic/necrotic cells (aCINVs) are internalized by in myelogenous (K562) 10-fold more efficiently than CINVs, and interact much less efficiently with melanocytic (B16) or epithelial (KB-3-1) cancer cells. Finally, we found that CINVs caused a temporal and reversible drop of the rate of cell division, which restored to the level of control cells with a 24 h delay.
The attention to the forearm fractures, as to osteoporotic fractures, is important for ensuring early detection of individuals at increased risk of future fractures and taking preventive measures.

In 20152017, in Novosibirsk, a random urban population sample of males and females, 5884 years old (n=3878), was surveyed. The study included persons who signed the informed consent to conduct the study, excluded individuals who wrote a waiver of taking blood to determine biochemical parameters. In total, the analysis included n=3393 people, 718 of them with DM2 (21.2%). Work design is cross-sectional research. The collection of information on fractures during for the last 3 years, the registration of socio-demographic data; and risk factors for NCD, a study of biochemical blood parameters. The analysis of the association of DM2 and a complex of risk factors for NCD with a chance of a forearm fracture.

The prevalence of forearm fractures in the last 3 years did not differ in patients with DM2 compared with those examined without diabetes and was 2.4% and 2.8%, respectively (p=0.557). Men with fractures had higher cholesterol and HDL values, women had lower body mass index (BMI), compared with people without fractures. According to the results of a multivariate analysis in women, the chance of a forearm fracture is directly associated with smoking in the past, a total cholesterol level of more than 200 mg/dl and inversely associated with a BMI. In men, associations were found of the chance of a forearm fracture with an increase in the level of cholesterol. There was no evidence of DM2 with forearm fracture. The obtained data on the incidence of fractures and their association with risk factors for chronic low risk infections suggest the need for preventive measures for osteoporotic fractures, both in people with and without DM2.
Data on the process of fractionation, mechanical activation and subsequent enzymatic hydrolysis of pea seeds proteins was obtained. As a result of fractionation of plant raw materials, fractions of biopolymers with different chemical composition were obtained. The conditions of mechanical activation for the protein fraction with the enzyme preparation, the optimal substrate-enzyme complex ratio and the optimal conditions for mechanically activated enzymatic hydrolysis were determined. It was shown that under certain optimal conditions for hydrolysis of pea biomass, the protein molecules broke down and a mixture of low molecular weight products formed.
We report the observation of $\mathrm{\ensuremath{\Upsilon}}(2S)\ensuremath{\rightarrow}\ensuremath{\gamma}{\ensuremath{\eta}}_{b}(1S)$ decay based on an analysis of the inclusive photon spectrum of $24.7\text{ }\text{ }{\mathrm{fb}}^{\ensuremath{-}1}$ of ${e}^{+}{e}^{\ensuremath{-}}$ collisions at the $\mathrm{\ensuremath{\Upsilon}}(2S)$ center-of-mass energy collected with the Belle detector at the KEKB asymmetric-energy ${e}^{+}{e}^{\ensuremath{-}}$ collider. We measure a branching fraction of $\mathcal{B}[\mathrm{\ensuremath{\Upsilon}}(2S)\ensuremath{\rightarrow}\ensuremath{\gamma}{\ensuremath{\eta}}_{b}(1S)]=(6.{1}_{\ensuremath{-}0.7\ensuremath{-}0.6}^{+0.6+0.9})\ifmmode\times\else\texttimes\fi{}{10}^{\ensuremath{-}4}$ and derive an ${\ensuremath{\eta}}_{b}(1S)$ mass of $9394.{8}_{\ensuremath{-}3.1\ensuremath{-}2.7}^{+2.7+4.5}\text{ }\text{ }\mathrm{MeV}/{c}^{2}$, where the uncertainties are statistical and systematic, respectively. The significance of our measurement is greater than 7 standard deviations, constituting the first observation of this decay mode.
Long-range interactions between cold Rydberg atoms are being investigated for neutral-atom quantum computing, quantum simulations, phase transitions in cold Rydberg gases and other applications. Fine tuning of the interaction strength can be implemented using Förster resonances between Rydberg atoms controlled by an electric field. The observation of the Stark-tuned Förster resonances between Rydberg atoms excited by narrowband cw laser radiation requires usage of a Stark-switching technique in order to excite the atoms first in a fixed electric field and then to induce the interactions in a varied electric field, which is scanned across the Förster resonance. The application of the radio-frequency field causes additional Förster resonances between collective states, whose line shape depends on the interaction strengths and time. Spatial averaging over the atom positions in a single interaction volume yields a cusped line shape of the Förster resonance. We present a detailed experimental and theoretical analysis of the line shape and time dynamics of the Stark-tuned Förster resonances Rb(nP3/2) + Rb(nP3/2) → Rb(nS1/2) + Rb([n + 1]S1/2) for two Rb Rydberg atoms interacting in a time-varying electric field.
Short-lived turquoise killifish (Nothobranchius furzeri) have become a popular model organism for neuroscience. In the present paper we study for the first time their behavior in the novel tank diving test and the levels of mRNA of various 5-HT-related genes in brains of 2-, 4- and 6-month-old males and females of N. furzeri. The marked effect of age on body mass, locomotor activity and the mRNA level of Tph1b, Tph2, Slc6a4b, Mao, Htr1aa, Htr2a, Htr3a, Htr3b, Htr4, Htr6 genes in the brains of N. furzeri males was shown. Locomotor activity and expression of the Mao gene increased, while expression of Tph1b, Tph2, Slc6a4b, Htr1aa, Htr2a, Htr3a, Htr3b, Htr4, Htr6 genes decreased in 6-month-old killifish. Significant effects of sex on body mass as well as on mRNA level of Tph1a, Tph1b, Tph2, Slc6a4b, Htr1aa, 5-HT2a, Htr3a, Htr3b, Htr4, and Htr6 genes were revealed: in general both the body mass and the expression of these genes were higher in males. N. furzeri is a suitable model with which to study the fundamental problems of age-related alterations in various mRNA levels related with the brains 5-HT system.
In modern concepts, the upper mantle of the Earth is a highly viscous incompressible liquid, and its flow is described using the Navier – Stokes equations in the Oberbeck – Boussinesq and geodynamic approximations. Convective flows in the upper mantle play a decisive role in the kinematics of lithospheric plates and the geological history of continental regions. Mathematical modeling is a basic method for studying convective processes in the mantle. Our paper presents a numerical model of convection, which is based on the implicit artificial compressibility method. This model is tested in detail by comparing our calculation results with the results of a well-known international test. It is demonstrated that the Fedorenko grids sequence method is highly efficient and reduces the computing time almost by a factor of eight. The numerical model is generalized in order to state the problem in a spherical system of coordinates. It is used to analyse the distribution of convective flows in the upper mantle underneath the Eurasian continent. The analysis shows that the thickness and geometrical parameters of the lithospheric blocks are the factors of significant influence on the distribution of convective flows in the upper mantle. The resulting structure of convective flows is manifested in the surface topography of large platform areas wherein the lithosphere thickness is increased. Thus, the locations of extended downward convection flows under the East European and Siberian platforms are clearly comparable to syneclises observed in the study area.
In this article we propose the numerical solution of the one dimensional inverse coefficient problem for seismic equation.

We use a dynamical version of Gelfand-Levitan-Krein approach for reducing a nonlinear inverse problem for recovering the shear wave's velocity and the density of the medium to two sequences of the linear integral equations. We propose numerical algorithm for solving these equations based on a fast inversion of a Toeplitz matrix.

The proposed numerical methods base on the structure of the problem and therefore improve the efficiency of the algorithms, compared with standard approaches. We present numerical results for solving considered integral equations.