Hengzongshu/ArticleAgent huggingface dataset:
Graphical abstractAbstractThe Drosophila S2 tissue culture cells are a widely used system for studies on mitosis. S2 cells are particularly sensitive to gene silencing by RNA interference (RNAi), allowing targeted inactivation of mitotic genes. S2 cells are also well suited for high-resolution light microscopy analysis of mitosis in fixed cells, and can be easily immunostained to detect mitotic components. In addition, S2 cells are amenable to transformation with plasmid encoding fluorescently tagged mitotic proteins, allowing in vivo analysis of their behavior throughout cell division. However, S2 cells have not been widely used for transmission electron microscopy (TEM) analysis, which provides ultrastructural details on the morphology of the mitotic apparatus that cannot be obtained with high-resolution confocal microscopy. Here, we describe a simple method for the ultrastructural analysis of mitosis in Drosophila S2 cells.•Our method, which involves fixation and sectioning of a cell pellet, provides excellent preservation of mitotic structures and allows analysis of a higher number of mitotic divisions per sample, compared to correlative light-electron microscopy.•Dividing cells are randomly oriented within the pellet and are sectioned along different planes, providing all-around information on the structure of the mitotic apparatus.
The translation start site plays an important role in the control of translation efficiency of eukaryotic mRNAs. The recognition of the start AUG codon by eukaryotic ribosomes is considered to depend on its nucleotide context. However, the fraction of eukaryotic mRNAs with the start codon in a suboptimal context is relatively large. It may be expected that mRNA should possess some features providing efficient translation, including the proper recognition of a translation start site. It has been experimentally shown that a downstream hairpin located in certain positions with respect to start codon can compensate in part for the suboptimal AUG context and also increases translation from non-AUG initiation codons.

We evaluated interdependency between the start codon context and mRNA secondary structure at the CDS beginning: it was found that a suboptimal start codon context significantly correlated with higher base pairing probabilities at positions 13 – 17 of CDS of human and mouse mRNAs. Thus, we have developed a tool, AUG_hairpin, to predict local stem-loop structures located within the defined region at the beginning of mRNA coding part. The implemented algorithm is based on the available published experimental data on the CDS-located stem-loop structures influencing the recognition of upstream start codons.

Prediction of such a compensatory hairpin may be useful in the evaluation of eukaryotic mRNA translation properties. It is likely that the downstream hairpins are used to enhance translation of some mammalian mRNAs in vivo. An occurrence of a potential secondary structure downstream of start AUG codon in a suboptimal context (or downstream of a potential non-AUG start codon) may provide researchers with a testable assumption on the presence of additional regulatory signal influencing mRNA translation initiation rate and the start codon choice. AUG_hairpin, which has a convenient Web-interface with adjustable parameters, will make such an evaluation easy and efficient.
We overview recent research trends in cancer genomics, bioinformatics tools development and medical genetics, based on results discussed in papers collections "Medical Genetics, Genomics and Bioinformatics"
Based on the analysis of various reports of the large world organizations, the article shows that energy forecasting is an important part of forecasting social development, as it largely determines the development vectors of other sectors of national economy and the world as a whole. The paper gives the description and classification of energy development forecasts, i.e. the description of goals, benchmarks and scenarios, global economic situation during the forecast implementation period, etc. It is shown that many organizations have changed the prerequisites, guidelines and the vision of future energy development over time; as a result, the possible options for the development of world energy have been revised. The major uncertainties in the dynamics have led to the emergence of many forecast options, whereas new developments in the world give rise to new types of forecasts. Changes in the technological and spatial structure of generation, the structure of fuel and energy consumption, energy capacity, regional shifts in energy in the period 2000-2018 were analyzed. Key trends in energy development in Russia and the world were highlighted. It is shown that the conflict between new technologies and the previous energy systems is dampened solely due to the support of clean energy at both national and world economic levels by the major actors of the international community.
Apurinic/apyrimidinic (AP) sites, a prominent type of DNA damage, are repaired through the base excision repair mechanism in both prokaryotes and eukaryotes and may interfere with many other cellular processes. A full repertoire of AP site-binding proteins in cells is presently unknown, preventing reliable assessment of harm inflicted by these ubiquitous lesions and of their involvement in the flux of DNA metabolism. We present a proteomics-based strategy for assembling at least a partial catalogue of proteins capable of binding AP sites in DNA. The general scheme relies on the sensitivity of many AP site-bound protein species to NaBH(4) cross-linking. An affinity-tagged substrate is used to facilitate isolation of the cross-linked species, which are then separated and analyzed by mass spectrometry methods. We report identification of seven proteins from Escherichia coli (AroF, DnaK, MutM, PolA, TnaA, TufA, and UvrA) and two proteins from bakers' yeast (ARC1 and Ygl245wp) reactive for AP sites in this system.
This paper reviews foreign methods for assessing an organizational climate, i.e. a complex of organizational, managerial, economic, socio-psychological, and other conditions. Organizational climate affects staff creativity and organizational innovation. The authors propose methods and tools for assessing the creative and innovative climate of Russian organizations. The methods can become an important element of systemic and regular monitoring of the organizational climate and its improvement. The survey of respondents (employees) was performed on the Google Forms platform; data processing and presentation of the results were performed with the help of Google Tables tools. The proposed methodology makes it possible to check the reliability and accuracy of the results. It offers a set of rules for automatic conclusions about the state of organizational climate based on the data obtained from interviewing the staff. The proposed methods and instruments may be put into practice of organizations in order to obtain reliable estimates of their organizational climate.
Such helmets were common in Central Asia between the late 16th and the mid-18th centuries. This specimen was likely manufactured in Mawarannahr, Xinjiang, or some town on the Syr Darya, for a high-ranking Uzbek, Uyghur, or Kazakh warrior. This accounts for the combination of a solid crown and a hoop with Arabic inscriptions with a box-type visor typical of helmets worn by Mongolian and Turkic nomads during the Late Middle Ages and the Early Modern Age.

We describe a richly decorated iron helmet owned by the Northern Kazakhstan Regional Museum in Petropavlovsk. It consists of a low solid hemispherical crown, a slightly convex plate, made of copper alloy, with an opening for a (missing) tube in which the plume was inserted, a wide iron hoop, and a bipartite visor of the box type. The two last-named elements are covered with Arabic inscriptions inlaid in gold. Those on the hoop are verses from the Quran 2, 255–257, Al-Baqarah — The Cow. That on the "shield" of the visor is a prayer for protection, known as the "message of peace" read before a long journey or a difficult and dangerous enterprise, such as a battle.

The closest parallels are found in the museums of Kazakhstan. Judging by the traces of repair and reconstruction, this helmet was used for a long time.
NMR hyperpolarization techniques have the potential to revolutionize the field of NMR spectroscopy and molecular MRI because they can transiently enhance nuclear spin polarization by 4–8 orders of magnitude, with corresponding gains in NMR signal‐to‐noise ratio (SNR). The SABRE‐SHEATH (Signal Amplification By Reversible Exchange in SHield Enables Alignment Transfer to Heteronuclei) technique, first demonstrated in 2015, allows for direct, efficient (>20 % nuclear spin polarization), and fast (in under one minute) hyperpolarization of 15 N sites. Several classes of biologically relevant 15 N hyperpolarized contrast agents have been efficiently hyperpolarized to date including pH sensors, which can be potentially useful for non‐invasive pH imaging of cancer and other diseases with altered metabolism. Here, we report the optimized 15 N enrichment of imidazole‐ 15 N 2 – a promising in vivo pH sensor with pKa ∼ 7.0. A hyperpolarized 0.1 M aqueous solution (ϵ 15N ∼ 146,000 fold, P 15N ∼ 0.24 %) was used to record 15 N NMR spectra at 0.05 T, demonstrating the feasibility of high‐resolution (full width at half maximum ∼ 1 Hz corresponding to 5 ppm at 0.05 T) NMR spectroscopy near its pKa (7.0) at ultra‐low magnetic field. Given that proton‐binding events modulate the chemical shift by ∼ 30 ppm for this pH‐sensing probe, our results demonstrate the feasibility of ultra‐low‐field pH sensing near its pKa (7.0) with SNR approaching that of high‐field (9.4 T) MR.
Novel hybrid magnetoactive composite scaffolds based on poly(3-hydroxybutyrate) (PHB), gelatin, and magnetite (Fe3O4) were fabricated by electrospinning. The morphology, structure, phase composition, and magnetic properties of composite scaffolds were studied. Fabrication procedures of PHB/gelatin and PHB/gelatin/Fe3O4 scaffolds resulted in the formation of both core-shell and ribbon-shaped structure of the fibers. In case of hybrid PHB/gelatin/Fe3O4 scaffolds submicron-sized Fe3O4 particles were observed in the surface layers of the fibers. The X-ray photoelectron spectroscopy results allowed the presence of gelatin on the fiber surface (N/C ratio-0.11) to be revealed. Incubation of the composite scaffolds in saline for 3 h decreased the amount of gelatin on the surface by more than ~75%. The differential scanning calorimetry results obtained for pure PHB scaffolds revealed a characteristic melting peak at 177.5 °C. The presence of gelatin in PHB/gelatin and PHB/gelatin/Fe3O4 scaffolds resulted in the decrease in melting temperature to 168-169 °C in comparison with pure PHB scaffolds due to the core-shell structure of the fibers. Hybrid scaffolds also demonstrated a decrease in crystallinity from 52.3% (PHB) to 16.9% (PHB/gelatin) and 9.2% (PHB/gelatin/Fe3O4). All the prepared scaffolds were non-toxic and saturation magnetization of the composite scaffolds with magnetite was 3.27 ± 0.22 emu/g, which makes them prospective candidates for usage in biomedical applications.
Translation termination in eukaryotes is mediated by release factors: eRF1, which is responsible for stop codon recognition and peptidyl-tRNA hydrolysis, and GTPase eRF3, which stimulates peptide release.

Here, we have utilized ribose-specific probes to investigate accessibility of rRNA backbone in complexes formed by association of mRNA- and tRNA-bound human ribosomes with eRF1•eRF3•GMPPNP, eRF1•eRF3•GTP, or eRF1 alone as compared with complexes where the A site is vacant or occupied by tRNA.

Our data show which rRNA ribose moieties are protected from attack by the probes in the complexes with release factors and reveal the rRNA regions increasing their accessibility to the probes after the factors bind. These regions in 28S rRNA are helices 43 and 44 in the GTPase associated center, the apical loop of helix 71, and helices 89, 92, and 94 as well as 18S rRNA helices 18 and 34. Additionally, the obtained data suggest that eRF3 neither interacts with the rRNA ribose-phosphate backbone nor dissociates from the complex after GTP hydrolysis. Taken together, our findings provide new information on architecture of the eRF1 binding site on mammalian ribosome at various translation termination steps and on conformational rearrangements induced by binding of the release factors.
By a conformal algebra we generally mean what is known as $H$-pseudo-algebra over the polynomial Hopf algebra $H=\Bbbk[T_1,..., T_n]$. Some recent results in structure theory of conformal algebras are applied

In this note, we introduce a class of algebras that are in some sense related to conformal algebras. This class (called TC-algebras) includes Weyl algebras and some of their (associative and Lie) subalgebras

to get a description of TC-algebras
Nickel-containing mixed ceria-zirconia oxides also doped by Nb and Ti have been prepared by a citrate route and by original solvothermal continuous flow synthesis in supercritical alcohols. Nickel was subsequently deposited by conventional insipient wetness impregnation. The oxides are comprised of ceria-zirconia solid solution with cubic fluorite phase. Negligible amounts of impurities of zirconia are observed for samples prepared by citrate route and doped by Ti. Supports prepared by supercritical synthesis are single-phased. XRD data, Raman, and UV-Vis DR (diffuse reflectance) spectroscopy suggest increasing lattice parameter and amount of oxygen vacancies in fluorite structure after Nb and Ti incorporation despite of the preparation method. These structural changes correlate with the catalytic activity in a methane dry reforming reaction. Catalysts synthesized under supercritical conditions are more active than the catalysts of the same composition prepared by the citrate route. The catalytic activity of samples doped with Ti and Nb is two times higher in terms of TOF (turnover frequency) and increased stability of these catalysts is attributed with the highest oxygen mobility being crucial for gasification of coke precursors.
Early development osteoporosis is a typical feature of OXYS rats.

The comparative study of activity of cysteine protease cathepsin K and matrix metalloproteases (MMPs) in bone tissue of accelerated senescent OXYS rats with early ageing comparatively to Wistar rats of the same age was performed. In bone tissue of 3 month old OXYS rats, before appearance of osteoporosis manifestation cathepsin K activity was higher, whereas MMPs activity was lower than in Wistar rats. In Wistar rats (3 and 14 months old) cathepsin K activity of spine was shown to increase, and MMPs activity to decrease. In OXYS rats age-related change of cathepsin K and MMPs activity in bone tissue had the opposite direction. Serum α2-macroglobulin activity was higher in 14 months old OXYS rats.

As a result of this there were no differences between Wistar and OXYS rats 14 months old despite the marked osteoporosis in OXYS rats as revealed our early researches. The role of activation of cathepsin K in bone resorption in the development of osteoporosis in early ageing OXYS rats is discussed.
Iterative processes in the Krylov subspaces for solving large ill conditioned saddle-type SLAEs with sparse matrices arising in finite difference, finite volume, and finite element approximations of multidimensional boundary value problems with complex geometric and functional properties of the initial data, characteristic of many relevant applications are studied. Combined two-level iterative algorithms using efficient Chebyshev acceleration and variational the conjugate directions methods, as well as the Golub-Kahan bi-diagonalization algorithms in the Krylov subspaces are considered. Examples of two-dimensional and three-dimensional filtration problems are used to study the resource consumption and computational performance of the proposed algorithms, as well as their scalable parallization on the multiprocessor systems with distributed and hierarchical shared memory.
We propose and evaluate through extensive numerical modelling a novel distributed hybrid amplification scheme combining first and second-order Raman pumping

which gives reduced signal power excursion over a wide spatial-spectral range of 80 nm in C+L-bands
We investigated phase relations, mineral chemistry, and density of lunar highland anorthosite at conditions up to 125 GPa and 2000 K.

We used a multi-anvil apparatus and a laser-heated diamond-anvil cell for this purpose. In-situ X-ray diffraction measurements at high pressures and composition analysis of recovered samples using an analytical transmission electron microscope showed that anorthosite consists of garnet, CaAl4Si2O11-rich phase (CAS phase), and SiO2 phases in the upper mantle and the mantle transition zone. Under lower mantle conditions, these minerals transform to the assemblage of bridgmanite, Ca-perovskite, corundum, stishovite, and calcium ferrite-type aluminous phase through the decomposition of garnet and CAS phase at around 700 km depth.

Anorthosite has a higher density than PREM and pyrolite in the upper mantle, while its density becomes comparable or lower under lower mantle conditions. Our results suggest that ancient anorthosite crust subducted down to the deep mantle was likely to have accumulated at 660–720 km in depth without coming back to the Earth's surface. Some portions of the anorthosite crust might have circulated continuously in the Earth's deep interior by mantle convection and potentially subducted to the bottom of the lower mantle when carried within layers of dense basaltic rocks.
φ-meson production in e + e --annihilation at VEPP-2M collider

The conversion decays φ → ηe + e -, φ → π 0 e + e -and η → γe + e -were studied by the SND detector

The e + e --pair mass spectra and transition form factors for φ → ηe + e -and η → γe + e -were also studied
The swirl is produced using a Turbomeca swirler and air flow, while combustion is supported by a central methane/air jet.

We studied flow dynamics in a model combustion chamber using Large-eddy simulations (LES) and Particle image velocimetry (PIV) at the Reynolds number Re of 15,000. We compared four flow regimes, assessing the effect of the central jet for isothermal and lean reacting conditions. A detailed comparison for isothermal and reactive cases without the central jet is described, validating the LES results against PIV.

We observe that unsteady dynamics are governed by global instability in the form of a well-known precessing vortex core (PVC). The central jet slightly changes the dynamics of PVC in the isothermal case where a strong recirculation zone is still formed. However, for the reacting case, the bubble is completely destroyed with no signs of strong vortical structures in the inner shear layer. These observations are confirmed using spectral analysis and proper orthogonal decomposition, describing the contribution of different flow modes in terms of azimuthal harmonics.
The divanadium-substituted polyoxometalate (Bu4N)5−nHn[γ-PW10V2O40] (n = 1–2) is an efficient and recyclable catalyst used for the synthesis of coenzyme Q0 using hydrogen peroxide as a green oxidant.
The photoelectron processes in a p -GaN(Cs) photocathode with the effective negative electron affinity were studied experimentally within the 90–295 K temperature range.

It was found that the photocathode illumination at the photon energies, which are below the energy gap of the p -GaN layer, increases the band bending at a semiconductor surface due to the photoemission from surface states.
The liver fluke Opisthorchis felineus is a member of the triad of epidemiologically important liver fluke species belonging to the family Opisthorchiidae and the major agent causing opisthorchiasis over a vast territory, covering Russia, Kazakhstan and several European countries. The similarity between the diseases caused by O. felineus and other liver flukes, O. viverrini and Clonorchis sinensis, in clinical manifestations and course suggests that the scenarios of their development and, possibly, complications have much in common. The International Agency for Research on Cancer classified O. viverrini and C. sinensis as group 1 agents and the major factors inducing cholangiocarcinoma in endemic regions. However, a carcinogenic potential of O. felineus is poorly studied. This review characterizes O. felineus, briefs the epidemiological situation in Western Siberia, the world's largest opisthorchiasis focus, and assesses the carcinogenic potential of this liver fluke. The review is based on a comprehensive analysis of the published medical data on opisthorchiasis and its complications in Western Siberia. Results of performed analysis reflect the actual epidemiological situation in opisthorchiasis focus and suggest an association of this disease with bile duct cancer.
The paper discusses the results of an experimental study of the propagation of the “N-wave” in a supersonic flow and its interaction with the bow shock wave, which is formed at flowing around a flat plate with a sharp leading edge. It was found that the “N-wave” does not change its direction when interacting with the bow shock. However, this interaction leads to a reduction of the “N-wave”. Estimates of the propagation angles of the “N-wave” in a supersonic flow are also obtained.
Large amplitude internal solitary waves in a continuously stratified fluid are discussed analytically.

Long-wave scaling procedure is used in order to construct approximate plateau-shape solitary waves possessing table-top crests and narrow fronts.

Existence of exact solutions is proved near the Boussinesq limit by means of generalized Lyapunov-Schmidt method.
In the developing brain, mature brain derived neurotrophic factor (mBDNF) and its precursor (proBDNF) exhibit prosurvival and proapoptotic functions, respectively. However, it is still unknown whether mBDNF or proBDNF is a major form of neurotrophin expressed in the immature brain, as well as if the level of active caspase-3 correlates with the levels of BDNF forms during normal brain development. Here we found that both proBDNF and mBDNF were expressed abundantly in the rat brainstem, hippocampus and cerebellum between embryonic day 20 and postnatal day 8. The levels of mature neurotrophin as well as mBDNF to proBDNF ratios negatively correlated with the expression of active caspase-3 across brain regions. The immature cortex was the only structure, in which proBDNF was the major product of bdnf gene, especially in the cortical layers 2-3. And only in the cortex, the expression of BDNF precursor positively correlated with the levels of active caspase-3. These findings suggest that proBDNF alone may play an important role in the regulation of naturally occurring cell death during cortical development.
Preeclampsia (PE) is one of the most common and serious complications of pregnancy. In women with a history of PE the risk of cardiovascular disease is increased, and atherosclerosis can be induced even during fetal development. The exact mechanisms by which PE increases future cardiovascular risk are unknown, although multiple similarities between mechanisms responsible for cardiovascular disease and PE are reported.

Risk factors for PE, such as obesity, insulin resistance, thrombophilia, changes in the lipid spectrum are similar to those for atherosclerosis, which allows us to compare these two conditions to clarify the specifics and is important for understanding the pathogenesis of both pathologies. PE, as well as atherosclerosis, manifests as endothelial dysfunction, abnormal immune function, oxidative stress, activation of inflammation, changes in lipid metabolism.

Recent studies have provided a broader understanding of the problem, although there are still many open questions. The etiology and pathogenesis of these diseases, their possible relationship are not fully understood. The article provides a summary of possible common mechanisms of PE and atherosclerosis.