Hengzongshu/ArticleAgent huggingface dataset:
Background: Tissue-engineered vascular grafts of small diameter have being widely used in coronary artery bypass grafting. However, the rate of settling of these grafts with endothelial cells is insufficient. Aims:The aim of the study was to selucidate the adhesion of endothelial progenitor cells and mesenchymal stem cells to synthetic materials (polystyrol, polytetrafluoroethylene) preprocessed with extracellular matrix (gelatin, fibronectin, collagen) in vitro.Materials and methods: For the study,the endothelial progenitor cells (EPC) were isolated from the peripheral blood of patients with ischemic heart disease, and mesenchymal stem cells (MSC) — from bone marrow of Wistar rats. Commercial monoclonal antibodies for flow- cytometry were used to determine the phenotype of EPC. To isolate the early and late EPC from mononuclear cells of peripheral blood, cells were raised for 8 and 16 days on a gelatin or fibronectin based substrate. The commercial kits for enzyme linked immunoassay were applied to assess levels of cytokine production and nitric oxide by early and late EPC conditioned by gelatin or fibronectin on the 8th and 16th days of growth. To conduct the study the MSC were isolated from bone marrow of rats. To determine the attachment of adherent fraction of nucleated bone marrow cells, cell morphology, the adipogenic and osteogenic differentiation were evaluated. To assess migration of MSC in real time within 24 hours on the device Cell-IQ, we used «closure/wound healing» test. The commercial kits for enzyme linked immunoassay were applied to assess levels of cytokine production and nitric oxide by MSC; and components of the extracellular matrix (fibronectin, collagen I and type IV) ― to assess the increased adhesion of MSC to the polytetrafluoroethylene. Results: The results demonstrated that endothelial progenitor cells adhere to both gelatin and fibronectin and confirmed the influence of these extracellular matrix components on the cytokine levels produced by early and late endothelial cells. The combination of fibronectin with type I or IV collagen or the combination of thereof promotes the adhesion to polytetrafluoroethylene and colonization of the graft.Conclusions: Preprocessing of synthetic material (polystyrene, polytetrafluoroethylene) enhances adhesion and growth of EPC and MSC which can be implemented when creating tissue-engineered vascular grafts for small diameter coronary artery bypass grafting with specified conditions of settlement by the cells involved in neointima formation.
This paper is devoted to the description of a new block cipher that will be applicable in the post-quantum era and will not need a lot of resources.

All this combined with the use of PRNG, working on the Cremona transformations

The main advantages: probabilistic encryption, the cipher block chaining mode, the ability to transfer to distributed systems. has significantly increased the cryptographic strength and increased the scope of this encryption.
Urine of prostate cancer patients contains tumor-specific biopolymers, including protein- and microvesiclesassociated miRNAs that can potentially be used as oncomarkers. Previously we have characterized urine extracellular vesicles and demonstrated diagnostic potential of their miRNA cargo.

In this study, we have performed a comparative analysis of the expression of 84 miRNA in paired samples of urine microvesicles and clarified urine from healthy men, patients with benign hyperplasia and cancer of the prostate using miRCURY LNA miRNA qPCR Panels.

Subsets of miRNAs with differences in expression between the fractions of the urine were found in all three groups. Two groups of miRNA were identified based on the patterns of their differential expression. They regulate several key signaling pathways associated with prostate cancer development.
The research featured various types and strains of lactic acid bacteria (LAB) and yeast. The research objective was to study their ability to utilize β-lactoglobulin during sourdough fermentation.

A set of experiments with various types and strains of LAB showed that β-lactoglobulin decreased in gluten-free sourdough with 30%, 60%, and 90% of skimmed milk powder (SMP). L.plantarum E36 demonstrated the highest biodegradation of β-lactoglobulin (by 53%) with SMP = 30%. L.helveticus ATCC8018T showed the lowest content of β-lactoglobulin with SMP = 60% and 90%: the content fell by 48% and 40%, respectively. The largest decrease in the content of β-lactoglobulin was observed in the sourdough with Saccharomyces cerevisiae 17 (by 28–42%) and Candida milleri Pushkinsky (by 25–41%). The content of total protein increased, which was not associated with yeast biomass growth. The content was determined after fermentation in sourdoughs with SMP = 60% and 90% using a bicinchoninic acid reagent kit.

The content of β-lactoglobulin in the control and experimental samples did not exceed 1 μg/g in the finished bakery products. This fact indicated a significant effect of thermal treatment on β-lactoglobulin degradation in baking. Thus, temperature processing (baking) had a greater impact on the destruction of β-lactoglobulin than enzymatic processing (fermentation).
A collection of introgression lines was obtained from crosses of common wheat (Triticum aestivum L.) cultivars with tetraploid wheat Triticum timopheevii (Zhuk.). Evaluation of resistance to fungal diseases revealed the lines with resistance to leaf and stem rusts, powdery mildew, spot blotch, and loose smut, the most widespread in Siberian region of Russia. Localization of the T. timopheevii genome fragments by means of microsatellite markers determined higher frequency of substitutions and translocations on chromosomes 1А, 2A, 2B, 5A, 5B and 6B. Molecular mapping of the loci determining leaf rust resistance revealed two independent loci on chromosomes 5B and 2A. The major locus on 5BS.5BL-5GL translocated chromosome accounting 64% of the phenotypic variance of the trait was found to be closely linked to microsatellite markers Xgwm814 and Xgwm1257. The other, minor locus, controlling 11% of the trait was mapped next to Xgwm312 on chromosome 2A. Microsatellite markers located near these genes may be used for controlling the transfer of valuable traits in new wheat cultivars.
A search for the process $e^+e^- \to \eta^\prime$ has been performed with the SND detector at the VEPP-2000 $e^+e^-$ collider.

The data were accumulated at the center-of-mass energy of $957.78\pm 0.06$ MeV with an integrated luminosity of about 2.9 pb$^{-1}$. For reconstruction of the $\eta^\prime$ meson five decay chains have been used: $\eta^{\prime}\to\eta\pi^+\pi^-$ followed by the $\eta$ decays to $\gamma\gamma$ and $3\pi^0 $, and $\eta^{\prime} \to \eta\pi^0\pi^0$ followed by the $\eta$ decays to $\pi^+\pi^-\pi^0$, $\gamma\gamma$, and $3\pi^0$.

As a result, the upper limit has been set on the $\eta^\prime$ electronic width: $\Gamma_{\eta^{\prime}\to e^+e^-} < 0.0020$ eV at the 90\% confidence level.
Neurofeedback training (NFT) to decrease the theta/beta ratio (TBR) has been used for treating hyperactivity and impulsivity in attention deficit hyperactivity disorder (ADHD); however, often with low efficiency. Individual variance in EEG profile can confound NFT, because it may lead to influencing non-relevant activity, if ignored. More importantly, it may lead to influencing ADHD-related activities adversely, which may even result in worsening ADHD symptoms. Electromyogenic (EMG) signal resulted from forehead muscles can also explain the low efficiency of the NFT in ADHD from both practical and psychological point-of-view. The first aim of this study was to determine EEG and EMG biomarkers most related to the main ADHD characteristics, such as impulsivity and hyperactivity. The second aim was to confirm our hypothesis that the efficiency of the TBR NFT can be increased by individual adjustment of the frequency bands and simultaneous training on forehead muscle tension.
One of the crucial elements contributing to the adaptation of organisms to unfavorable environmental conditions is the reaction of stress. The study of its genetic control and role in adaptation to unfavorable conditions are of special interest. The juvenile hormone (JH) acts as a gonadotropic hormone in adult insects controlling the development of the ovaries, inducing vitellogenesis and oviposition. It was shown that a decrease in JH degradation in individuals reacting to adverse conditions by stress reaction (R­individuals) causes delay in egg laying and seems to allow the population to 'wait out' the unfavorable conditions, thereby contributing to the adaptation at the population level. However, monitoring natural populations of D. melanogaster for the capability of stress reaction demonstrated that they have a high percentage of individuals incapable of it (NR­individuals).

To verify the hypothesis by an experiment, the fitness characteristics (lifespan, fecundity) of the R and NR lines of D. virilis were studied under normal conditions and under regular heat stress of various frequency.

The study of reproductive characteristics of R­ and NR­individuals showed that under normal conditions R­individuals have the advantage of procreating offspring. Under unfavorable conditions, if the stressor is intense enough, NR­individuals die, but if its intensity is low, then they, unlike R­individuals, continue to produce offspring. Based on these data, it was hypothesized that the balance of R­ and NR­alleles in the population ensures its adaptation under frequent stresses of low intensity.
We report a measurement of time-dependent $CP$ violation parameters in ${B}^{0}\ensuremath{\rightarrow}{K}_{S}^{0}\ensuremath{\eta}\ensuremath{\gamma}$ decays. The study is based on a data sample, containing $772\ifmmode\times\else\texttimes\fi{}{10}^{6}B\overline{B}$ pairs, that was collected at the $\mathrm{\ensuremath{\Upsilon}}(4S)$ resonance with the Belle detector at the KEKB asymmetric-energy ${e}^{+}{e}^{\ensuremath{-}}$ collider. We obtain the $CP$ violation parameters of $\mathcal{S}=\ensuremath{-}1.32\ifmmode\pm\else\textpm\fi{}0.77(\mathrm{stat})\ifmmode\pm\else\textpm\fi{}0.36(\mathrm{syst})$ and $\mathcal{A}=\ensuremath{-}0.48\ifmmode\pm\else\textpm\fi{}0.41(\mathrm{stat})\ifmmode\pm\else\textpm\fi{}0.07(\mathrm{syst})$ for the invariant mass of the ${K}_{S}^{0}\ensuremath{\eta}$ system up to $2.1\text{ }\text{ }\mathrm{GeV}/{c}^{2}$.
Nodal and Activin are morphogens of the TGFbeta superfamily of signaling molecules that direct differential cell fate decisions in a dose- and distance-dependent manner. During early embryonic development the Nodal/Activin pathway is responsible for the specification of mesoderm, endoderm, node, and mesendoderm. In contradiction to this drive towards cellular differentiation, the pathway also plays important roles in the maintenance of self-renewal and pluripotency in embryonic and epiblast stem cells. The molecular basis behind stem cell interpretation of Nodal/Activin signaling gradients and the undertaking of disparate cell fate decisions remains poorly understood.

Here, we show that any perturbation of endogenous signaling levels in mouse embryonic stem cells leads to their exit from self-renewal towards divergent differentiation programs. Increasing Nodal signals above basal levels by direct stimulation with Activin promotes differentiation towards the mesendodermal lineages while repression of signaling with the specific Nodal/Activin receptor inhibitor SB431542 induces trophectodermal differentiation. To address how quantitative Nodal/Activin signals are translated qualitatively into distinct cell fates decisions, we performed chromatin immunoprecipitation of phospho-Smad2, the primary downstream transcriptional factor of the Nodal/Activin pathway, followed by massively parallel sequencing, and show that phospho-Smad2 binds to and regulates distinct subsets of target genes in a dose-dependent manner.

Crucially, Nodal/Activin signaling directly controls the Oct4 master regulator of pluripotency by graded phospho-Smad2 binding in the promoter region. Hence stem cells interpret and carry out differential Nodal/Activin signaling instructions via a corresponding gradient of Smad2 phosphorylation that selectively titrates self-renewal against alternative differentiation programs by direct regulation of distinct target gene subsets and Oct4 expression.
Summary: Solutions containing mixtures of methylalumoxane (MAO) and i Bu 3 Al give rise to 1 H NMR signals indicative of the presence of the mixed alkyl aluminum dimers i Bu 2 Al(µ‐Me) 2 Al i Bu 2 and of mixed clusters of the type (AlMe (1 + 2 x − y ) i Bu y O (1 − x ) ) n . These mixed clusters, as well as related species present in solutions containing either MAO–Et 3 Al or commercially available modified MAO (MMAO), appear to have stronger Lewis acidic sites and greater hydrodynamic radii than comparable clusters present in solutions of MAO alone, as judged from EPR signals observed in these solutions upon addition of TEMPO. When (SBI)ZrCl 2 (SBI = rac ‐Me 2 Si(ind) 2 ) is reacted with one of these mixed activator reagents, the mixed heterobinuclear cation [(SBI)Zr(µ‐Me) 2 AlMe i Bu] + appears to be formed, together with its methyl counterpart [(SBI)Zr(µ‐Me) 2 AlMe 2 ] + , which is normally predominant in the (SBI)ZrCl 2 /MAO system at [Al] MAO /[Zr] ratios above 100. In the presence of i Bu 3 Al, the formation of heterobinuclear cations is suppressed in favor of ion pairs containing the cation [(SBI)ZrMe] + in contact with a (MAO–TIBA)‐derived counter anion. The greater reactivity of these contact‐ion pairs, as compared to the normally prevalent AlMe 3 adducts, as well as an increased Lewis acidity of MMAO, and the ensuing decreased coordination ability of the [Me‐(MAO–TIBA)] − counter ion as compared to [Me‐MAO] − , are likely to contribute to the positive effects of TIBA additions on the co‐catalytic activity of MAO. magnified image
Vaviloid spike branching, also called sham ramification, is a typical trait of Triticum vavilovii Jakubz. and is characterized by a lengthening of the spikelet axis.

In this article, we present the results of a study of three triticale-wheat hybrid lines with differences in terms of the manifestation of the vaviloid spike branching. Lines were obtained by crossing triticale with hexaploid wheat, T. aestivum var. velutinum. The parental triticale is a hybrid of synthetic wheat (T. durum × Ae. tauschii var. meyrei) with rye, S. cereale ssp. segetale. Line 857 has a karyotype corresponding to hexaploid wheat and has a spike morphology closest to normal, whereas Lines 808/1 and 844/4 are characterized by the greatest manifestation of vaviloid spike branching. In Lines 808/1 and 844/4, we found the substitution 2RL(2DL). The karyotypes of the latter lines differ in that a pair of telocentric chromosomes 2DS is detected in Line 808/1, and these telocentrics are fused into one unpaired chromosome in Line 844/4. Using molecular genetic analysis, we found a deletion of the wheat domestication gene Q located on 5AL in the three studied hybrid lines. The deletion is local since an analysis of the adjacent gene B1 showed the presence of this gene.

We assume that the manifestation of vaviloid spike branching in two lines (808/1 and 844/4) is associated with a disturbance in the joint action of genes Q and AP2L2-2D, which is another important gene that determines spike morphology and is located on 2DL.
By means of a hollow plasma, multiple proton bunches work well in driving nonlinear plasma wakefields and accelerate electrons to the energy frontier with preserved beam quality. However, the acceleration of positrons is different because the accelerating structure is strongly charge dependent. There is a trade-off between keeping a small normalized emittance and a small energy spread.

By loading an extra electron bunch to repel the plasma electrons and meanwhile reducing the plasma density slightly to shift the accelerating phase with a conducive slope to the positron bunch, the positron bunch can be accelerated to 400 GeV (40% of the driver energy) with an energy spread as low as 1% and well preserved normalized emittance.

The successful generation of high quality and high energy positrons paves the way to the future energy frontier lepton colliders.
In this work we propose a new physical realization of optical neural network (ONN) based on a recently appeared technological platform of synthetic photonic lattices (SPL), and demonstrate its capabilities for deep learning. The system operates with time series of optical pulses with ability to easily control their parameters and possesses the architecture that well suits the ONN paradigm. We have also shown that such an ONN can be potentially utilized for signal processing in optical communication lines for signal distortion compensation.
The X-band Electron Paramagnetic Resonance (EPR) spectroscopy endstation at the Novosibirsk Free Electron Laser (NovoFEL) facility is capable for steady-state (CW) EPR and time-resolved (TR) continuous wave EPR experiments.

Recently several upgrades in experimental hardware and software have been done, aiming to more productively use the time of experimental session and to increase the control of experimental parameters during the session. Firstly, we installed detectors with different time resolution for tracking radiation produced by NovoFEL. Secondly, on the basic of fsc2 program, we implemented a possibility of a simultaneous use of two oscilloscopes, configured at different timescale and/or sensitivity.

Finally, we developed software for on-the-fly CW and TR experimental data viewing and treatment.
The subject of this paper is molecular modelling of the transport processes in liquids including nanofluids. The weak and strong nonequilibrium processes are considered. In the first case, the corresponding fluctuation-dissipation theorems are formulated. These theorems determine the relation between the dissipative processes and equilibrium thermal fluctuations of the mass, momentum, and energy in the molecular system.

Two different formulations of these theorem are discussed in detail. Various systems are considered: molecular liquids, nanofluids, turbulent liquids, fluids in confined conditions, and microviscosity in biomembranes.

In the last part the nonlinear transport processes and rheology of the non-Newtonian fluids are discussed.
The results of a high-resolution Raman scattering study of a diamond crystal with a high content of single substitutional nitrogen impurities (550 ppm) in the temperature range from 50 to 673 K are presented and compared with the data for defect-free diamond. It is established that the increase of the nitrogen concentration in diamond leads to the temperature-independent increase of the Raman line width. Analysis of the experimental data allows us to conclude that this broadening should be attributed to the defect-induced shortening of the Raman phonon lifetime. We believe that this mechanism is responsible for the increase of the Raman line width caused by most point-like defects in diamond. No pronounced effects of the nitrogen defects on the Raman line position and phonon anharmonicity are observed.
In recent years, using a genome-wide association study (GWAS), a number of single nucleotide polymorphisms (SNPs) have been suggested to be associated with susceptibility to leukemia in cattle. However, all studies have been done with purebred Holstein cows and their hybrids. In this regard, it is important to confirm the functional role of polymorphisms previously identified in a GWAS study in Russian cattle breeds.

The aim of this study was to verify the association between rs110861313 in the intergenic region of bovine chromosome 23 and leukemia in the Russian Black Pied cattle. Based on the levels of bovine leukemia virus (BLV)-specific antibodies detected in serum using serodiagnostic techniques, animals were divided into three groups: healthy animals ( n = 115), asymptomatic virus carriers ( n = 145) and animals with leukemia ( n = 107). Genotyping of rs110861313 was carried out using polymerase chain reaction followed by analysis of restriction fragment length polymorphisms.

A significant decrease in the frequency of the A/A genotype (11.2 %) was revealed in animals with persistent lymphocytosis compared to virus carriers (27.6 %) ( p < 0.002). At the same time, the frequency of animals with the C/C genotype in animals with persistent lymphocytosis (41.1 %) was significantly higher than that of virus carriers (21.4 %) ( p < 0.001). In this case, asymptomatic virus carriers can be considered a more suitable control than healthy animals that have not been in contact with the virus. According to bioinformatics analysis, resistance to BLV can be due to the presence of the transcription factor FOXM1 binding site in the region of rs110861313. FOXM1 is expressed in immune cells and can potentially affect the expression of the neighboring genes ( LY6G5B, GPANK1, ABHD16A, LY6G6F, LY6G6E, CSNK2B, ApoM ). Thus, we found that SNP rs110861313 in the intergenic region of bovine chromosome 23 is associated with the development of leukemia following BLV infection in the Russian Black Pied cattle.
Empedocles uses two forces to describe the world process, the emergence and destruction of space - Love and Strife, which work in turn, and in due time, replace each other. It is obvious that love is responsible for unification and creation, and hostility for division and destruction. At first glance it seems quite natural that it is the power of unification that Empedocles calls Aphrodite. However, when you look closely at the fragments of the poem, the image of Aphrodite is not so unambiguous: she acts as a god-craftsman, that is, not just watches from afar as the roots of things are connected to each other, but mixes them with her own hands and is directly involved in the creation of living beings. We meet her involved in such activities as metal casting, pottery, and artwork. This naturally leads to the question from where did she get so many different functions? To answer this question, one should turn to literary sources about Aphrodite both before and after Empedocles’ life (in the context of Homer’s epos and Hesiod’s poem), consider the religious tradition of Cyprus and especially the East, neighboring Greece, from where, in the opinion of some scientists, the goddess could get into the Mediterranean cultural landscape (most important study here is the work by Nano Marinatos), to study archaeological data and findings related to Aphrodite. Taking into account Empedocles’ interest to bloodless sacrifices I will try to tie his views with the later orphic tradition. At the same time, in order to protect myself from losing the way in the forest of such huge massif it is necessary to restrict the area of this study. I will concentrate only on the activities of Aphrodite as she is presented in Empedocles.
The Laser Polarimeter is being developed at VEPP-4M collider for beam energy calibration by Resonant Depolarization technique.

The essential part of this facility is a photon-tracking detector measuring coordinates of backscattered gammas.