Biosphere 2 contained representative biomes: a 1,900 square meter rainforest, an 850 square meter ocean with a coral reef, a 450 square meter mangrove wetlands, a 1,300 square meter savannah grassland, a 1,400 square meter fog desert, a 2,500 square meter agricultural system, a human habitat, and a below-ground infrastructure.
Stekleni objekt je dvignjen skoraj 1300 m nad morsko gladino ob vznožju gorovja Santa Catalina, pol ure stran od Tucsona.