One of the early Portuguese masters of the 16th century was Marçal de Matos, to whom "Susanna and the Elders" (1565), in Quinta da Bacalhoa, Azeitão, is attributed, as well as the "Adoration of the Shepperds" (in the National Museum of Azulejos in Lisbon).
Kljub temu so bili vse do sredine 16. stoletja Portugalci še naprej odvisni od uvoza, večinoma iz Španije, v manjšem obsegu iz Antwerpna (na primer dva panela Jana Bogaertsa v Paço Ducal, Vila Viçosa, Alentejo) in Italije ("Oznanjenje" Francisca Niculosa v Évori in Orazio Fontana).