Major customers have included Aerolíneas Argentinas, Aeroméxico, Aeropostal Aerorepublica, Alaska Airlines, Alitalia, Allegiant Air, American Airlines, Aserca, Austral Líneas Aéreas, Austrian Airlines, Avianca, China Eastern Airlines, China Northern Airlines, Delta Air Lines, Finnair, Iberia, Japan Air System (JAS), Korean Air, Lion Air, Martinair Holland, Pacific Southwest Airlines (PSA), Reno Air, Scandinavian Airlines System (SAS), Spanair, Insel Air, Swissair, and Trans World Airlines.
Главные пользователи MD-80:* Bukovyna* AeroMexico* Allegiant Air* Northwest Airlines* American Airlines* Austrian Airlines* Avianca* Delta Air Lines* Swissair* Alitalia* Scandinavian Airlines System (SAS)* Finnair* UM Air* Iberia* Japan Air System (JAS)* China Eastern Airlines* Midwest Airlines* China Northern Airlines* Alaska Airlines* Korean Air* Khors Air Company* Austral.