From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: D.pseudoobscura Adh gene for alcohol dehydrogenase
Date: 28 Dec 92 21:26:11 GMT
Distribution: bionet
Checksum: 62342 8

LOCUS       DROADHGN02   3502 bp ds-DNA             INV       01-DEC-1992
DEFINITION  D.pseudoobscura Adh gene for alcohol dehydrogenase
ACCESSION   X62182
KEYWORDS    ADH gene; alcohol dehydrogenase.
SOURCE      Drosophila pseudoobscura DNA.
  ORGANISM  Drosophila pseudoobscura
            Eukaryota; Animalia; Metazoa; Arthropoda; Uniramia; Insecta;
            Pterygota; Neoptera; Holometabola; Diptera; Brachycera;
            Cyclorrhapha; Schizophora; Drosophiloidea; Drosophilidae.
REFERENCE   1  (bases 1 to 3502)
  AUTHORS   Schaeffer,S.W. and Miller,E.L.
  TITLE     Estimates of gene flow in Drosophila pseudoobscura determined from
            nucleotide sequence analysis of the alcohol dehydrogenase region
  JOURNAL   Genetics 132, 471-480 (1992)
  STANDARD  full automatic
COMMENT     *source: strain=BC86; *source: isolate=British Columbia, Canada;
            *source: chromosome=IV section 88;
               From EMBL    entry DPADHGN02;  dated 16-OCT-1992.
FEATURES             Location/Qualifiers
     mRNA            join(40..119,924..1029,1093..1497,1558..1973)
                     /note="adult mRNA (Adh)"
     mRNA            join(891..1029,1093..1497,1558..1973)
                     /note="larval mRNA (Adh)"
     promoter        8..14
                     /note="adult promoter (Adh)"
     prim_transcript 40..1973
                     /note="adult primary transcript (Adh)"
     exon            40..119
                     /note="adult exon 1a"
     intron          120..923
                     /note="adult intron"
     promoter        855..862
                     /note="larval promoter (Adh)"
     prim_transcript 891..1029
                     /note="larval primary transcript (Adh)"
     exon            891..1029
                     /note="larval exon 1"
     exon            924..1029
                     /note="adult exon 1b"
     intron          1030..1092
                     /number=1
     exon            1093..1497
                     /number=2
     intron          1498..1557
                     /number=2
     exon            1558..1973
                     /number=3
     polyA_signal    1950..1955
     polyA_site      1973
     CDS             join(937..1029,1093..1497,1558..1824)
                     /EC_number="1.1.1.1"
                     /gene="Adh"
                     /product="alcoholdehydrogenase"
                     /codon_start=1
                     /translation="MSLTNKNVVFVAGLGGIGLDTSRELVKRNLKNLVILDRIDNPAA
                     IAELKAINPKVTITFYPYDVTVPVAETTKLLKTIFAQVKTIDVLINGAGILDDHQIER
                     TIAVNYTGLVNTTTAILDFWDKRKGGPGGIICNIGSVTGFNAIYQVPVYSGSKAAVVN
                     FTSSLAKLAPITGVTAYTVNPGITKTTLVHKFNSWLDVEPRVAEKLLEHPTQTSQQCA
                     ENFVKAIELNKNGAIWKLDLGTLEPITWTQHWDSGI"
BASE COUNT      978 a    802 c    799 g    923 t
ORIGIN      
        1 cgtagtgtat ttaaggcact gcacatgtcg aggaccacga ttattgtcta cagtgcagtc
       61 agtagttgtc aggtgcagca ggcggaccag catttcttta aatctactca atcgatcaag
      121 taagtaactg taatccaagt acagtcggcc catcccactc ggtcactcgg ttctgccgta
      181 actcaagaaa tttcttgata gcgcagttcc agttgtaaaa cgtcgtttaa aaaaacatct
      241 cgttggagct tgggttgaaa aacgttcgag ttgggcgtag aaaagctcaa atataaaata
      301 tcaaaaaaga gaaaatatct tcccaaaagc agctgtccat tttttgttaa agtgatagat
      361 caaaaattcg tatgacgtat ttcccaagtg ctcttcatgc aaataatgtc ctcaattcta
      421 attctacaag aagcactttt ggctcttgtc cagaaaaaac aatttgttta cacttttcgg
      481 ttgataacag aagcggaccg acggctaagt aacataacag tcccctgtta agtaatgccc
      541 tcactctctg ttagatggcc gcgcgtgtcg actccgatag accagcagac acgcagtagt
      601 tttcctcaag aacctgaact ctaaacatag acataattta cgataccttg agactcaaat
      661 aaaaatacaa gtatgagtgg tattctattc ctgattagac tcgcacacac acacgtacga
      721 gtattaacat aaatttgccg gcaaataacc aaaacaaaac gaacgcaaac gatccgaaca
      781 cgtaatgcga gagataagaa acagaaaagc tcgacgtgaa agaagcttct ggcggaagct
      841 cttgccgaac tccctataaa tacgagacag aaaaccagaa taaatataat aacaaactgt
      901 tgaattgtcc catcgattcc cagatcaaaa gacagaatgt ctctcaccaa caagaacgtc
      961 gttttcgtgg ccggtctggg cggcattggc ctggacacca gccgggagtt ggtcaagcgt
     1021 aatctgaagg taagagagaa cgaattccat ggaatctata gaatcctaaa tttaaaaatt
     1081 catttatttt agaacctggt cattctggat cgcattgaca atccggctgc cattgccgag
     1141 ctgaaggcaa tcaatcccaa ggtgaccatc accttctatc cctacgatgt gactgtgccc
     1201 gtcgctgaga ccaccaagct cctgaagacc atctttgccc aggtgaagac catcgatgtc
     1261 ctgatcaacg gtgctggcat cctggacgat catcagatcg agcgcaccat tgccgttaac
     1321 tacacgggcc tggtcaacac caccacagcc attctggact tctgggacaa gcgcaagggc
     1381 ggcccaggcg gcatcatttg caacattggc tccgtcaccg gtttcaatgc catctaccag
     1441 gtgcccgttt actccggctc caaggcggcg gtggttaact ttacctcctc cctggcggta
     1501 agtaatccaa cgtttttcct actacccaaa actaaatatt tctttgaaat cccatagaaa
     1561 ctggctccca ttactggtgt cactgcttac actgtcaatc ctggcatcac taagaccact
     1621 ctggtccaca aattcaactc gtggctggat gtggagcccc gtgtggcaga gaagctgctc
     1681 gagcatccca cccagacctc tcagcagtgc gccgagaact ttgtgaaggc catcgagctg
     1741 aacaagaacg gtgccatctg gaagttggat ctgggcacct tggagcccat cacatggacc
     1801 cagcactggg actcgggcat ctaaaccatc ccagagactc tatgggacat ggcgttttag
     1861 cttttagttt ctttttttcc actcaattgt tacgtatata catacacata tggcaataag
     1921 gctgatttga accccgtttt gaatatgata atacaaaatt atatttgaga aatttcaaca
     1981 aaatcgataa gagctatagc tctcttttaa ttaaaaaaaa ctagataaaa agagcaatgg
     2041 tcagtggtgg tcagtggtcg ttgtggcctc tatcgatttc cacacaaaaa ctttactgtt
     2101 tagtaataga aaagaacttg agaaaggcag ccaaaagaat gtacgatctg acgggtaagc
     2161 atgtctgcta tgtggctgac tgcggtggca ttgcactgga gaccagcaag gttctcatga
     2221 ccaagaatat agcggtgagt ggtagagagg tggtgtagag agtggagagt gttccgaagt
     2281 ggttctggtt cttaaacaag tctctagtct ctagtctcta gtctctagtc tcttggctgc
     2341 tgctagtagc tgtcgaaact aattaagtta atgacttttt catgttttgt ttttgtccgc
     2401 tgacttttgc tgttcaaagc tacaattaag tttatgtttt gagtttgatc tacttttgct
     2461 taagggaagt actttcgatt acggattgct ggcatataaa aatagatacc aaggaatgtg
     2521 cctcatatca tattgattct atcttgattc cttgcccaga aactggccat ccttcagagc
     2581 gtggaaaacc ccccggccat cgctcagtta caatcgatta agcacagcac acagatcttc
     2641 ttctggacct tcgatgtgac catggcccgc gaggagatga agaagtactt cgatgaggtc
     2701 atggtccaga tggactacat cgatgtgctg atcaatgggg ccaccctgtg cgatgagcgg
     2761 aacattgatg ccaccatcaa tacgaatctg accggcatga tgaacaccgt ggccactgtg
     2821 ctgccctata tggaccgaaa gatgggcggt tcgggtggac tgattgtgaa tgtcacctcc
     2881 gtcataggac tggatccatc gccagttttt tgtgcgtaca gtgcctcaaa gtttggtgtg
     2941 attgggttca cccgaagtct agcggtgagt gggcgagatc ttaacttgaa aatgctctct
     3001 aatgtcttcc attctttcgc aggatcccct gtattacacc caaaatggtg tggctgtcat
     3061 ggccgtctgc tgtggtccca ccaaagtgtt tgtcgatcgg gaactgactg ccttcttgga
     3121 atacggccag tcctttgccg atcgcctgcg tcgtgccccc tgccaatcga cggccgtctg
     3181 cggccagaat atagtaaatg ccatcgagag atcggagaac ggacagatct ggattgccga
     3241 caagggcggc ctggagtcgg tggccctcca ctggtattgg catatggccg atcagtttgt
     3301 gaactacatg cagagcactg acgatgagga tcaggagttt tttttggggc agcgatgagg
     3361 catgccttga tccacggaat aaaacatcaa atgtgtcaca aaaagtaggc aacttttttg
     3421 tgtgtttgga aatagatttg aaatattttg aggtcatatg aataatgaca taaatgaggg
     3481 tgcctgaaag tatgccaagt tt
//