From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: B.stearothermophilus pheB gene for catechol-2,3-dioxygenase
Date: 4 Sep 92 10:02:33 GMT
Distribution: bionet
Checksum: 34639     4

LOCUS       BACPHEB      1393 bp ds-DNA             BCT       04-SEP-1992
DEFINITION  B.stearothermophilus pheB gene for catechol-2,3-dioxygenase
ACCESSION   X67860
KEYWORDS    catechol 2,3-dioxygenase; pheB gene.
SOURCE      Bacillus stearothermophilus DNA.
  ORGANISM  Bacillus stearothermophilus
            Prokaryotae; Firmicutes; Endospore-forming rods and cocci;
            Bacillaceae.
REFERENCE   1  (bases 1 to 1393)
  AUTHORS   He,Z.Q., Mao,Y.M., Sheng,Z.J. and Shen,R.Q.
  TITLE     Complete nucleotide sequence of pheB gene encoding catechol 2,
            3-dioxygenase from bacillus stearothermophilus
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
COMMENT     *source: strain=FDTP-3;
            From EMBL    entry BSPHEB;  dated 13-AUG-1992.
FEATURES             Location/Qualifiers
     -35_signal      264..269
     -10_signal      288..293
     RBS             377..382
     CDS             391..1374
                     /EC_number="1.13.11.2"
                     /product="catechol 2,3-dioxygenase"
                     /gene="pheB"
                     /codon_start=1
                     /translation="MSKNFQEPIFDVAQLAHVELLSPKLEESIVFFTKYLGMEVTARA
                     GNSVYLRAYEDFYHNTLKITESAEAGLGHVGWRASSPQALERRVLELEKSGLGRGWID
                     GDIGHGKAYQFTTPDGHQMEIFFEVEYYKPQPEQKTKLLNRPSKRPAQGVPVRRLDHI
                     NLMTSNPGVDTQFMIDTLGFRLREQIRDKGKILGSWISVSNLVHEIAFMQEPNQEKGK
                     LHHLCYWYGIPQNLYDLADLLKDHEYFIEVPPNKHGISQAFCMYVYEPGGNRIELFGD
                     AGYLITDPTWEPVIWEMEDVPGNGDTWIGTAFPDSWWLRGTPVTTKEVVKP"
BASE COUNT      443 a    249 c    310 g    391 t
ORIGIN
        1 agctcggcat taaagcttgt ggtgatttca attggggaaa gagcacggat gttaaacaaa
       61 ataaaagaaa gtcaaaaatt tgctgtcaat attttagcct gcaaatcaac aggaattatc
      121 aatgatgttt gcaggtcaaa tgaaagaaaa aagagatgtc cgttttgaac aacttgccgg
      181 tcttccagtt attcaaggtg cagttgctca agtggcatgt gaggttgctg gtgaacatgt
      241 agaaggcgac cacacccttt ttatcggtaa agtaaacgct attcaactat atgatgaaga
      301 accacttatt ttctttagtg gtcaatatca ttcgttagaa ccaaaggaag ctgtactcag
      361 taaataaatt attaataggt ggaatctata atgtcaaaaa atttccaaga accaatcttt
      421 gatgttgcac aattggctca tgtagagctt ctttctccaa aactagagga atccattgta
      481 ttttttacaa aatatttagg aatggaagta actgctcgcg cagggaattc tgtctatctt
      541 cgtgcgtatg aagatttcta tcataatact ctaaaaataa cggaatccgc tgaagctggt
      601 ttagggcatg ttggttggcg tgcgtcttct cctcaagcac ttgaaagacg ggtactggaa
      661 cttgaaaagt cgggtttagg ccgcggctgg attgatggag atattggcca tggcaaggca
      721 tatcaattta cgacacctga tggtcatcaa atggaaatat tttttgaggt cgaatattat
      781 aaaccacaac ctgagcaaaa gacaaaatta ttaaatcggc cgtctaaacg accagcacaa
      841 ggcgtacctg tccgccggtt agatcatatt aacttaatga cctctaaccc aggagtagat
      901 acgcaattta tgatcgatac gttaggattc cgcttaaggg aacaaatccg agataaaggc
      961 aaaatccttg gcagttggat tagtgtctca aacttagtac atgaaattgc gtttatgcaa
     1021 gagccgaatc aagaaaaagg gaagcttcat catctttgct attggtatgg catcccgcaa
     1081 aacctatatg atttagcaga cctattaaaa gaccatgaat atttcattga agttccacca
     1141 aataaacatg gaattagtca agcgttttgt atgtatgtct atgaaccagg tggcaatcga
     1201 atcgaattat ttggtgatgc cgggtatctt attaccgatc caacatggga gcctgttata
     1261 tgggagatgg aggatgtacc aggaaatggc gacacatgga tcggaaccgc tttcccagac
     1321 tcttggtggt tacgtggcac acctgttaca acgaaagaag ttgtgaagcc ataggtttgt
     1381 agggtaccga gct
//