From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Rhizobium meliloti phnG gene, 3' end cds; phnH, phnI, phnJ, and
Date: 21 Dec 92 20:35:17 GMT
Distribution: bionet
Checksum: 19803 10

LOCUS       RHMPHNG      4214 bp ds-DNA             BCT       19-NOV-1992
DEFINITION  Rhizobium meliloti phnG gene, 3' end cds; phnH, phnI, phnJ, and
            phnK genes, complete cds's.
ACCESSION   M96263
KEYWORDS    phnG gene; phnH gene; phnI gene; phnJ gene; phnK gene;
            phosphonate utilization.
SOURCE      Rhizobium meliloti (strain 1021) DNA.
  ORGANISM  Rhizobium meliloti
            Prokaryotae; Gracilicutes; Scotobacteria; Rhizobiaceae.
REFERENCE   1  (bases 1 to 4214)
  AUTHORS   McLean,P.A., Liu,C.-M., Sookdeo,C.C. and Cannon,F.C.
  TITLE     Characterization of a gene cluster involved in utilization of
            glyphosate and other phosphonates in Rhizobium meliloti
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     repeat_region   160..208
                     /note="contains the repeat unit TTCTTCGC twice, a variant
                     TTCTTAGC, plus 2 incomplete units"
     stem_loop       207..232
                     /note="palindromic pho box at -35"
     -35_signal      209..230
                     /note="-35 motif replaced with pho box"
     promoter        209..244
                     /note="-35 replaced by pho box, which is positively
                     regulated by phoB"
     -10_signal      239..244
                     /note="predicted relative to pho box"
     misc_feature    249..285
                     /note="73% a and t region between promoter and RBS"
     CDS             <394..764
                     /function="phosphonate utilization"
                     /standard_name="PhnG protein"
                     /note="PhnG protein"
                     /gene="phnG"
                     /codon_start=3
                     /translation="WDAIDDKPEVAPVRGPETGLVMVRGKIGGGGDPFNLGEATVSRA
                     TVRLSTGEVGHGQLLGTDKARARLAAIFDALFQSAAHRASVEALHEQVAARLDAEDRR
                     KAEETAATRVDFFTMVRGED"
     CDS             764..1366
                     /function="phosphonate utilization"
                     /standard_name="PhnH protein"
                     /note="PhnH protein"
                     /gene="phnH"
                     /codon_start=1
                     /translation="MTAQSQIYSGAFADPVFEAQSVFRSLMDCFARPGIIGRLSTAAA
                     PPAPLGEASGAVALTLCDHDTPVWLSPALSKSSAPKWIAFHTGAGVTDTKDEPRFAFF
                     EKGAAVPGFDQFALGTQEYPDRSTTLVVEVEALEGGQPLIGRGPGIKNGIVIAPKGLP
                     DVFLDLWAANRAIFPRGIDLVLTAREAVLCLPRTTKLERE"
     CDS             1371..2477
                     /standard_name="PhnI protein"
                     /standard_name="phosphonate utilization"
                     /note="phosphonate utilization"
                     /gene="phnI"
                     /codon_start=1
                     /translation="MYVAVKGGEAAIANAHRLLADRRRGDRALPAITIDQVVEQLGLA
                     VDRVMAEASLYDRALAALAVRQARGDMIEAIFILRAYRTTLPRFGYCEPVDTAKMKVE
                     RRVSATYKDLPGGQLLGPTFDYTHRLLDPSLIGEEPVDEPVLKEPGEHVMRVSDILDG
                     EGLIEGDGKMPEGHVAGDLTREPMEFPMARDLRLQALARGDEGFLLALAYSTQRGYGR
                     THPFVGEVRIGEVEVELELPELGFAVSLGVIRVTECQMVNQFKGSSRQPPQFTRGYGL
                     VFGQSERKAMSMSLVDRALRTDEFDEDIVAPAQDQEFVISHADNVQATGFVEHLKLPH
                     YVDFQAELDLVRRMRREHDAAQAGGKDGMKEAAE"
     CDS             2474..3367
                     /function="phosphonate utilization"
                     /standard_name="PhnJ protein"
                     /note="PhnJ protein"
                     /gene="phnJ"
                     /codon_start=1
                     /translation="MNDLATYNFAYLDEQTKRMIRRAILKAIAIPGYQVPFASREMPM
                     PYGWGTGGVQVTASILGPDDVLKVIDQGADDTTNAVSIRAFFQKVADVAVTTRTTEAT
                     IIQTRHRIPEEQLREGQTLVYQVPIPEPLRFLEPRETETRKMHALEEYGLMHVKLYED
                     IARNGHIATTYAYPVKVEGRYVMDPSPTPKFDNPKMHMSEALQLFGAGREKRIYAVPP
                     YTEVVSLDFEDHPFEVQKFDKPCALCGAENVYLDEVVLDDKGGRMFVCSDTDHCEDRR
                     AHGHAGAMLAPAAKESQEAAE"
     CDS             3364..4140
                     /function="phosphonate utilization"
                     /standard_name="PhnK protein"
                     /note="PhnK protein"
                     /gene="phnK"
                     /codon_start=1
                     /translation="MSAVPLLKVSDVSKFYGSRIGCRNVSFELYPGEVLAIVGESGSG
                     KTTLLSCLSTRLMPTSGIVEYHMRDGQYRDLARMSEAERRFLMRTDWGFVHQNPADGL
                     RMTVSAGANVGERLMAVGDRHYGNIRAAAGDWLRRVEIEEDRIDDQPRAFSGGMRQRL
                     QIARNLVTSPRLVFMDEPTGGLDVSVQARLLDLVRGLVHDLGLAAVIVTHDLAVARLL
                     SHRMMVMKDGAVIEQGLTDRVLDDPREPYTQLLVSSILQV"
BASE COUNT      743 a   1429 c   1331 g    711 t
ORIGIN      
        1 tgtcgctggt cgaccgggcg ctccggaccg ccgattccgc cgcaagggcc tctccggcgg
       61 caccatgccg gtggcgtcat agtcgccatt gctgatcgcg agccgtatcc tgtcggcaat
      121 ctgccgccat agcgccacgc ccatctgccg ttcgacgacc ttcgccacca tcttcttcgc
      181 cccgcttctt cgccgccagt ttcttagcaa atgtcacaag cctgtcattt gcgaaggtta
      241 tggaaaggta taagatgtat agttgtctag atcaatagac attttggagt gagatgatgg
      301 acgcagcgaa gacaagtgac gatgcaggcg tcgctcagcg ccgggaaggc atgcggctgc
      361 tggcgcgcgc gacgctcggc gaactttcct tggcctggga cgcgatcgac gacaagccgg
      421 aggtcgcgcc ggtgcgcggt ccggaaaccg ggctcgtcat ggtgcgcggc aagatcggcg
      481 gcggcggcga tcctttcaat ctcggcgagg ccaccgtttc acgcgccacc gtgcggcttt
      541 cgaccggcga ggtcggccat gggcagctgc tcggcaccga caaggcacgc gcccgccttg
      601 ccgcgatctt cgacgcgctt tttcagagcg ctgcgcatcg cgcaagcgtc gaggcgctgc
      661 acgagcaggt ggccgctcgt ctcgatgcgg aagaccgtcg caaggccgag gaaacggccg
      721 cgacccgcgt cgacttcttc accatggtcc gcggagagga ttgatgaccg cccaatcgca
      781 aatctatagc ggcgccttcg ccgatcccgt cttcgaggcg cagtccgtgt tccgcagcct
      841 gatggactgc ttcgcccgcc cgggcatcat cggccggctg tccactgctg cagccccgcc
      901 ggcgccactc ggagaggcaa gcggagctgt cgcgctcacg ctctgcgacc atgacacgcc
      961 ggtctggcta tcgccggcgc tctccaaatc ctcggcgccg aagtggatcg cgtttcacac
     1021 cggcgccggc gtcaccgaca cgaaggacga gccccgcttt gccttcttcg agaagggcgc
     1081 ggccgtgccg ggcttcgacc agttcgccct cggcacccag gaatatcccg accgttccac
     1141 cacgctcgtc gtcgaggtgg aagcgctcga aggcgggcag ccgctcatcg gtcggggacc
     1201 gggaatcaag aacgggatcg tcatcgcacc gaagggcctg cccgacgtgt tcctcgacct
     1261 ctgggccgcc aaccgggcga tctttccgcg cggcatcgat ctcgtcctga cggcgcgcga
     1321 ggcagtgctc tgcctgccgc gcaccaccaa actcgagcgg gagtaagcgc atgtatgtag
     1381 ccgtcaaggg cggagaagcc gccattgcca atgctcaccg gctgctggcc gaccgccggc
     1441 gcggcgaccg ggcgctgccc gcgatcacca tagatcaggt cgtcgagcag ctcgggctcg
     1501 cggtcgaccg cgtgatggcc gaggcctcgc tttacgaccg tgcgcttgcg gcgcttgccg
     1561 tgcgccaggc gcgcggagac atgatcgagg cgatcttcat cctgcgcgcc taccgcacca
     1621 ccctgccgcg cttcggctat tgcgaaccgg tcgacaccgc gaaaatgaag gtcgagcggc
     1681 gcgtctcggc gacctacaag gatctgcccg gcggccagct tctcggcccc accttcgact
     1741 atacgcaccg ccttctcgac ccgtcgctga tcggcgaaga accggtcgat gagccggtgc
     1801 tcaaggagcc gggcgaacat gtcatgcgcg tctccgacat tctcgacggc gaaggcctga
     1861 tcgagggcga cggcaagatg ccggaaggcc acgtggcggg cgacctcacg cgcgagccga
     1921 tggaatttcc gatggcgcgc gatctccgcc tgcaggcgct tgcccgtggc gacgaaggat
     1981 tcctgctggc gctcgcctac tccacgcagc gcggctatgg ccgcacgcac cccttcgtcg
     2041 gcgaggtcag gatcggcgag gtcgaggtcg aactggagct gccggagctg ggctttgccg
     2101 tttcgcttgg ggtcatccgc gttaccgaat gccagatggt caaccagttc aagggctcgt
     2161 ccaggcagcc gccgcaattc acccgcggct acgggctcgt cttcggccag agcgagcgca
     2221 aggcgatgtc gatgtcgctg gtcgaccggg cgctccggac cgacgaattc gacgaggaca
     2281 tcgtcgctcc cgcccaggat caggaattcg tcatctcgca tgccgacaac gtccaggcga
     2341 ccggcttcgt cgagcacctg aagctgccgc attacgtcga cttccaggcc gagctcgacc
     2401 tggtcaggcg catgcgccgg gaacacgacg ccgcccaggc cggcggcaag gacggcatga
     2461 aggaggccgc cgaatgaacg acctcgcaac ttacaacttc gcctatctgg acgagcagac
     2521 gaagcggatg atccgccgcg cgatcctgaa ggcgatcgcc attcccggct atcaggtgcc
     2581 cttcgcctcg cgcgaaatgc ccatgcccta tggctggggt accggcggcg tgcaggtgac
     2641 cgcctccatc ctcgggcccg acgacgtgct caaggtgatc gaccagggcg ccgacgacac
     2701 gacgaacgcc gtttcgatcc gcgccttctt ccagaaggtc gccgacgtcg ccgtcaccac
     2761 gaggacgacg gaggcgacga tcatccagac gcgccaccgc attcccgagg agcaactgcg
     2821 agaggggcag acgctcgtct accaggtgcc cattccggaa cccctgcgct tcctcgagcc
     2881 gcgcgaaacc gagacgcgca agatgcatgc gctggaagaa tacgggctca tgcatgtgaa
     2941 gctctacgag gacatcgccc gcaacggcca tatcgccacg acctacgcct atccggtaaa
     3001 ggtcgagggc cgttacgtga tggacccctc gccgacgccg aaattcgaca atccgaagat
     3061 gcatatgtcg gaggccctcc agctcttcgg cgccggccgg gagaagcgca tctatgcggt
     3121 gccgccctat accgaggtcg tcagcctcga tttcgaggat caccccttcg aggtccagaa
     3181 attcgacaag ccctgcgccc tttgcggcgc cgagaacgtc tatctcgacg aggtggtgct
     3241 cgacgacaag ggcgggcgca tgttcgtctg ctccgacacc gatcattgcg aggatcgccg
     3301 ggcgcacggc catgccggcg ccatgctcgc ccccgccgcg aaagaaagcc aggaggctgc
     3361 agaatgagcg ccgtgccgct tctgaaagtc agtgacgttt cgaaattcta tggaagccgc
     3421 atcggctgcc gcaacgtctc cttcgagctc tatccgggcg aggtgctcgc catcgtcggg
     3481 gaatccggat cgggcaagac cacgctgctc tcctgcctct cgacccggct gatgccgaca
     3541 tccggcatcg tcgaatatca catgcgcgac ggccaatacc gggacctcgc gcgcatgagc
     3601 gaggccgagc ggcgctttct gatgcgcacc gattggggct tcgttcacca gaacccggcg
     3661 gacgggctga ggatgacggt ttcggccggc gccaatgtcg gcgagcgttt gatggcggtc
     3721 ggggaccggc actacggcaa tatacgcgcg gcggcaggcg actggctgag gcgcgtggaa
     3781 atcgaagagg accggatcga cgatcagccg cgcgccttct ccggcggcat gcgccagcgc
     3841 ctgcagatcg cccgcaatct ggtcacctcg ccccgcctcg tcttcatgga cgagccgacc
     3901 ggcggcctcg acgtctcggt gcaggcacgc ctgctcgatc tcgttcgcgg gctcgtccac
     3961 gacctcggcc ttgcggcggt catcgtcacc cacgacctcg ccgtggcacg cctcctttcg
     4021 catcggatga tggtgatgaa ggacggcgcc gtcatcgagc agggtctgac cgaccgggtg
     4081 ctcgacgatc cgcgcgagcc ctacacgcag ctcctcgttt cctcgattct tcaggtgtga
     4141 cgcattgaca atgcgcaacg gaaagtaaag accatgccaa ctcccctcgt tgtctcggaa
     4201 gtcgcgaaaa gctt
//