From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: C.reflexa chloroplast petE, trnV, trnM, atpE, atpB and rbcL genes
Date: 26 Aug 92 10:00:15 GMT
Distribution: bionet
Checksum: 63493    13

LOCUS       CUTCPCRPET   6085 bp ds-DNA             ORG       26-AUG-1992
DEFINITION  C.reflexa chloroplast petE, trnV, trnM, atpE, atpB and rbcL genes
            for cytochrome b6/f-complex subunit V, tRNA-Val, tRNA-Met, ATP
            synthase epsilon- and beta-chains and ribulose-1,5-bisphosphate
            carboxylase large subunit, respectively.
ACCESSION   X61698
KEYWORDS    ATP synthase beta chain; ATP synthase epsilon chain; atpB gene;
            atpE gene; cytochrome b6/f-complex subunit V; petE gene; rbcL gene;
            ribulose bisphosphate carboxylase large subunit; transfer RNA-Met;
            transfer RNA-Val; trnM gene; trnV gene.
SOURCE      Chloroplast Cuscuta reflexa (chromosome Chloroplast Cuscuta
            reflexa) DNA.
  ORGANISM  Chloroplast Cuscuta reflexa
            Eukaryota; Plantae; Embryobionta; Magnoliophyta; Magnoliopsida;
            Asteridae; Polemoniales; Cuscutaceae.
REFERENCE   1  (bases 1 to 6085)
  AUTHORS   Haberhausen,G., Valentin,K. and Zetsche,K.
  TITLE     Organization and sequence of photosynthetic genes from the plastid
            genome of the holoparasitic flowering plant Cuscuta reflexa
  JOURNAL   Mol. Gen. Genet. 232, 154-161 (1992)
  STANDARD  full automatic
COMMENT     *source: strain=Roxb.;
            variation of 18-23 amino acids in rbc L as compared to other plants
            From EMBL    entry CHCRPETE;  dated 19-MAY-1992.
FEATURES             Location/Qualifiers
     CDS             complement(join(1277..1312,679..712))
                     /gene="trnV"
                     /codon_start=1
                     /translation="PRRSTVRVRIAWAIAQLVEHLVY"
                     /partial
     CDS             complement(38..151)
                     /product="cytochrome b6 /f-complexsubunit v"
                     /gene="petE"
                     /codon_start=1
                     /translation="MIEVFLFGIVLGLIPITLAGLFVTAYLQYRWGGWLDV"
     exon            679..712
                     /number=2
                     /gene="trnV"
     exon            1277..1312
                     /number=1
                     /gene="trnV"
     tRNA            complement(1470..1541)
                     /anticodon=(pos:unspecified,aa:Met)
                     /product="tRNA-Met"
                     /gene="trnM"
     CDS             complement(1633..2043)
                     /EC_number="3.6.1.34"
                     /note="epsilon chain"
                     /product="H(+)-transporting ATP synthase"
                     /gene="atpE"
                     /codon_start=1
                     /translation="MTLKLCVLTPNRIFWDSEVEEIILSTNSGQIGILPTHTPIATSV
                     DIGILRIRLNGRWLTMALMGGFARIGNNEITVLVNDAERSSDIDPQEAQQTVEIAEAN
                     FRKAKGKRQKIEANIALCRARTWVKAVSPISVGK"
     CDS             complement(2066..3538)
                     /EC_number="3.6.1.34"
                     /note="beta chain"
                     /product="H(+)-transporting ATP synthase"
                     /gene="atpB"
                     /codon_start=1
                     /translation="MRINPPTYGSEISSIEKKNRGHIVQIIGPVLDVAFPPGKMPNIY
                     NALIVKGRDTEQMNVTCEVQQLLGNNRVRAVAMNDTDGLMRGMEVIDTGTPITVPVGG
                     STLGRIFNVLGEPVDNLGPVDTNTTFTIHRSAPAFIQLDTKLSIFETGIKVVDLLAPY
                     RRGGKIGLFGGAGVGKTVLIMELINNIAKAHGGVSVFGGVGERTREGNDLYMEMKESG
                     VINEKNITESKVALVYGQMNEPPGARMRVGLTALTMAEYFRDVNEQDVLLFIDNIFRF
                     VQAGSEVSALLGRMPSAVGYQPTLSTEMGSLQERITSTKEGSITSIQAVYVPADDLTD
                     PAPATTFAHLDATTVLSRGLAAKGIYPAVDPLDSTSMMLQPRLVGEEHYETAQKVKQT
                     LQRYKELQDIIAILGLDELSEEDRLTVARARKIERFLSQPFFVAEVFTGSPGKYVGLA
                     ETIIGFTLILSGELDSLPEQAFYLVGNINEATEKAMNLKT"
     -35_signal      4142..4147
                     /gene="rbcL"
     -10_signal      4166..4171
                     /gene="rbcL"
     mRNA            4176..>5874
                     /gene="rbcL"
     CDS             4378..5874
                     /EC_number="4.1.1.39"
                     /product="ribulose bisphosphate carboxylase"
                     /gene="rbcL"
                     /codon_start=1
                     /translation="MSPQTETKASVGFKAGVKDYKLTYYTPDYETKDTDILAAFRVTP
                     QPGVPPEEAGAAVAAESSTGTWTTVWTDGLTSLDWYKGRCYRIERVIGEKDQYIAYVA
                     YPLDLFEEGSVTNLFTSIVGNVFGFKALRALRLEDLRIPPAYTKTFQGPPHGIQVERD
                     KLNKYGWPLLGCTIKPKLGLSAKNYGRAVYECLRGGLDFTKDDENVNSQPFMRWRDRF
                     LFCAEAIYKSQAETGEIKGHYLNATAGTCEEMLKRALFARELGVPIIMHDYLTGGFTA
                     NTSLAHYCRENGLLLHIHRAMHAVIDRQKNHGIHFRVLAKALRLSGGDHIHSGTVVGK
                     LEGEREITLGFVDLLRDDFVEQDRSRGIYFPQDWVSLPGVMPVASGGIHVWHMPALTE
                     IFGDDSVLQFGGGTLGHPWGNAPGAVANRVALEACVQAWNEGRDLAQEGNDILRQAGK
                     WSPELAAACEVWKEIRFDFKPVDTLDPNDKKQRDNEDTLADKLFGDKG"
BASE COUNT     1866 a   1149 c   1130 g   1940 t
ORIGIN
        1 gaattcaggg agcatataaa aaataacgtt taattaatca aacgtccaac cgaccccccc
       61 gcctgtattg taaatatgca gttacgaata agccagccaa agtaatagga attagaccta
      121 acacgattcc aaataaaaaa acttctatca tttaaatttg tttgaaagga taaaaaaata
      181 ggtaatatct aaacaccaaa ttgacatgaa tctcactgac cgagaattta caatgaattt
      241 ataattggtt cgacaagtaa ctgtaagtcc ctctagacta aacggacacg gaatcccgca
      301 ccacataaat ctttctttga aaagaaatat ttttttgacc taattgttca tttcaaaaat
      361 tcctttttct tttatttttt tgaaatttaa ataagtcgga tcttgttcag accaataaat
      421 agggctgagg ttatagtgaa agctgctact agaaaaccaa aataactagt tatagtaagc
      481 atgaataggc taatttaaat atgttcttta ttaggcatat gtttctaaag catttaccga
      541 agtttctatt tttttaatct tgactgggtc ctgagtccga tttcacaaca acaaacggtc
      601 agttccaaac aaaaaacaat aatgaagaaa ttcaaattag aaaatagaat atagaataga
      661 agttaactag aagttatagg gctatacgga ctcgaaccgt agaccttctc ggtaaaacag
      721 atcaaactac ttaattctaa aatatcaaaa tgatctgaac tatttcaaag acccaacccg
      781 catttttttc ctaagtctcc catctttttt cttgaaagaa aaataagaaa aagaaagggc
      841 tccttgtgct ccgattcatt ttttgagatt atgatccggg agcactaccg aagtgtttca
      901 tttcaagagc gggggggtta tcttgacgta ggtctgccta tggtctagat catcctaagt
      961 gaaatgaagt ctctaccctt tcaaaatcaa atatgaaact tgatacacct taaagttcag
     1021 ataatggaaa agatattttt ttgaggtcct tatcctcatt tcattaggcc taacattgaa
     1081 gaggctagca attcacccta tcaatatctc ctggagtctg tttggcacct aaatgggtgc
     1141 ccacccaact aaatcggaca cagttccttt gtccggctat ggttccctca aagttatgga
     1201 gtaagacata actaaaaaaa agatcccttt attatatcat tttaaaatgg acttccccga
     1261 acaacattgg cgcgcgtgta aacgaggtgc tctaccaact gagctatagc cccccttagt
     1321 gcttgtgatc tctattttat cccggagaga ttttctagtc aagatgacta ttatattctc
     1381 taaaatcggt attgcttata tggaatatta tctttataat ccagcgatgg gatgagggtt
     1441 tctttttgtt ctctttggga ttcgaaatta cctacttaac tcagtggtta gagtactgct
     1501 ttcatacggc gggaggcatt ggttcaaatc caatagtagg tagaacttat tagataccat
     1561 caagtcgggt atctaataag tttgttgacc caccctttat tttaatattt aattttagaa
     1621 tccttttttt atttatttac cgactgaaat cggactgacg gcctttaccc gtgttctagc
     1681 tcgacagaga gctatatttg cctcaatttt ttgtctcttt cctttagctt ttctaaaatt
     1741 agcttctgct atttcaacag tttgctgggc ttcttgggga tcaatgtcac tgctcctttc
     1801 tgcatcattt accaaaacag tgatttcatt attgcctatt ctagcaaacc cccccatcaa
     1861 agccattgtt aaccagcggc cattaagacg tattctcaaa atacctatat ctacagatgt
     1921 ggcaataggg gtgtgagttg gtaatattcc aatttgccca ctattagtag ataaaatgat
     1981 ttcttccact tctgaatccc aaaaaattct attaggggtc agtacacaaa gttttaaggt
     2041 catttaatta aattgctctc catttctaag tttttaagtt cattgccttc tcggtagctt
     2101 cattgatatt acctaccaaa taaaaagcct gttcaggaag actatctaat tcgccggaaa
     2161 ggatcaaagt aaaacctata attgtttcgg ctagaccaac atattttcct ggagaaccag
     2221 taaatacttc tgctacgaaa aagggttgtg ataagaaacg ctcaattttt cgcgctcttg
     2281 ctacggttaa acgatcctct tcagataatt cgtccaaccc aaggatcgct ataatgtcct
     2341 gaagttcttt gtaacgttgt aaagtttgct taactttttg cgcagtttca taatgttcct
     2401 caccaacgag gcgaggttga agcatcattg acgttgaatc taaaggatct actgctgggt
     2461 agataccttt agcagccaaa cctcttgata gtacggtagt tgcatctaaa tgtgcaaatg
     2521 tggtagcggg agcaggatcg gtcaaatcat ctgcaggtac ataaactgct tgaattgagg
     2581 ttatggaacc ttctttggtg gaagtaattc tttcttgtaa agaacccatt tctgtactca
     2641 aggtcggttg ataacccaca gcagaaggca ttcgacccag taaggccgat acttcggatc
     2701 ccgcttggac gaaacgaaag atattgtcga taaaaagaag tacgtcttgc tcattaacat
     2761 ctcggaaata ttccgccata gttagggcag tcaaaccaac tctcatgcga gctcccggcg
     2821 gttcattcat ctgaccgtaa actagagcca cttttgattc tgtaatattt ttttcgttaa
     2881 taactccgga ttctttcatt tccatgtaaa gatcatttcc ttcacgagta cgttcaccta
     2941 ctccgccaaa tacggatact cccccgtgag ctttggcaat attgttaatc aattccataa
     3001 tgagtactgt tttccccact ccagctcccc caaatagtcc gattttgcct ccgcggcgat
     3061 aaggggctaa aagatctact actttaattc ctgtttcaaa aatagataat tttgtatcca
     3121 actgtataaa ggcgggcgca gatctatgaa tagtaaacgt tgtattagta tctacaggac
     3181 ctaaattatc aacgggttct ccgagcacgt tgaaaattcg tcccagagtt gatccaccga
     3241 ccggaacagt tataggagtt cctgtgtcaa tcacttccat ccctctcatt agaccatctg
     3301 tatcattcat agctacagct ctaactcgat tatttcccaa taattgctgt acctcgcaag
     3361 tcacgttcat ttgttcagtg tctcgccctt tcactatcag agcattataa atattaggca
     3421 tcttgccagg gggaaaagct acatctagta ccggaccaat gatttggacg atatgccctc
     3481 ggtttttttt ttcaatcgag gaaatttcag aaccataagt aggaggattt attctcataa
     3541 caataataaa tttttttttt ttgcaaaact tttcgaatga aaaagaaatg tctgatatca
     3601 cgcggttaat ttaataagag aaaatgggaa tttgactcta ttttgttggt attatccaat
     3661 caaataaaat taaataaatt tttactgaat taaatgactt gaaggataat gggtcaatct
     3721 tttcaagttt acctaaccca tttttaaaat ctaaagtgca aataaaaagt ggataaagaa
     3781 aaatatttaa tcttggacaa gtctttcatc tggctatcat tctagaatta tataaattct
     3841 ataaaattct aaaaaattcc ttttttatta tagattatag ataatttgag ccttcaattt
     3901 tttgtatttc cattacgtta cgattcttga tttatatctc attggcgttt cctatttttt
     3961 tccttccagc atatgcatat gtattgattt acccctaatt attttttaat ttatttttga
     4021 aacctttaga ttttagagat aattttaaaa ttgtgtatat tttcacatct aggatttaca
     4081 tatacagcat aatctaggga attaggttag gttttaaaaa aagttaaaaa gaaaaagtga
     4141 gttgcactag atatatgaaa gagtgtacaa tattcatgta tttggaaaat aaatcaaata
     4201 cccttggtct cttggtctaa gaataaaata ttctcattcg ttgctaatat ttcgtattat
     4261 tagtatttag tatttttcaa agattccttt atttaatgaa cggcgaatta atgtcgagta
     4321 gaccttgttg ttgtgagaat tattaattat ttattcatga gttgtaggga ggaatttatg
     4381 tcaccacaaa cagagactaa agcaagtgtt ggattcaaag ctggtgttaa agactacaaa
     4441 ttgacttatt atactcccga ttacgaaacc aaagatactg atatcttggc agcattccga
     4501 gtcactcctc aacccggagt tccgcctgaa gaagccgggg ccgcggtagc cgcggaatct
     4561 tctactggta catggacaac tgtgtggacc gatggactta ccagccttga tcggtacaag
     4621 gggcgatgct accgcattga gcgcgttatt ggagaaaaag atcaatatat tgcttatgtc
     4681 gcttaccctt tagacctttt tgaagaaggt tctgttacca acctgtttac ttccattgtc
     4741 ggtaatgtat ttgggttcaa agcactgcgc gctttacgtc tggaagatct ccgaatacct
     4801 ccggcttata ctaaaacttt tcaaggcccg cctcacggca tccaagttga gagagataaa
     4861 ttgaacaagt atggtcggcc tctcttggga tgtactatta aaccaaaatt ggggttatcg
     4921 gctaaaaact acggtagagc ggtttatgaa tgtcttcgcg gtggacttga ttttaccaaa
     4981 gatgatgaga acgtaaactc acaaccattt atgcgttgga gagaccgttt cttattttgt
     5041 gccgaagcaa tttataaatc acaggctgaa acaggtgaaa tcaaaggaca ttacttgaat
     5101 gctactgcag gtacatgcga agaaatgcta aaaagagctt tatttgctag agaattggga
     5161 gttcctatta taatgcacga ctacttaaca gggggattca ctgcaaatac ttctttggct
     5221 cattattgcc gagagaatgg tctacttctt cacatccatc gtgcaatgca tgccgttatt
     5281 gatagacaaa agaatcatgg tatacacttc cgtgtactag cgaaagcgtt acgtctgtct
     5341 ggtggagatc atattcattc aggtactgta gtaggtaaac ttgaaggcga aagagaaatt
     5401 actttgggct ttgttgactt actacgcgat gattttgttg aacaagaccg aagtcgtggt
     5461 atttatttcc ctcaagattg ggtttcttta ccgggtgtta tgcccgtcgc ttcagggggt
     5521 attcacgttt ggcatatgcc ggctctgacc gagatctttg gggatgattc cgtactacag
     5581 ttcgggggag gaactttagg gcacccctgg ggtaatgcgc cgggtgccgt agctaatcga
     5641 gtcgctctag aagcatgtgt acaagctcgg aacgaaggtc gtgatcttgc tcaggaaggc
     5701 aatgacattc ttcgacaagc tggcaaatgg agccctgaac tagctgctgc ttgtgaggta
     5761 tggaaagaga ttcgatttga ctttaaaccc gtggatacct tggatccaaa tgataaaaaa
     5821 caaagagata atgaggatac cttagcggat aaattattcg gagataaggg atagggataa
     5881 ggaacttaag gaattacaac ctctccttct cttctgaatt gcaattaaac tcggcccaat
     5941 cttttactaa aatactaaag ggtaaaagaa tactaaaggg taaaagaatt gagccgaata
     6001 cagctaaata atttcttgga tgagtatatg agtataattt atctataaat attttagaaa
     6061 tcttttaaat aactttaatg aattc
//