HDTenEightyP/NetNews huggingface dataset:
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human chromosome 4 sequence-tagged site STS4-174
Date: 21 Aug 92 10:07:17 GMT
Distribution: bionet
Checksum: 09280     3

LOCUS       HUM4STS174    421 bp ds-DNA             PRI       21-AUG-1992
DEFINITION  Human chromosome 4 sequence-tagged site STS4-174
ACCESSION   M95246
KEYWORDS    STS; human chromosome 4; sequence tagged site.
SOURCE      Homo sapiens, clone C4-104 from Los Alamos National Laboratory
            chromosome 4 cosmid library, plate 4-1-1R, pos 2E; vector SuperCos
            1 (Stratagene)
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 421)
  AUTHORS   Goold,R.D., diSibio,G., Dugaiczyk,A., Smith,K.A., Xu,H.,
            Magrane,G., Lang,D.B., Cox,D.R., Masters,S.B. and Myers,R.M.
  TITLE     Sequence-Tagged Sites for Human Chromosome 4
  JOURNAL   Unpublished (1992) see COMMENT for author address
  STANDARD  full automatic
COMMENT     PCR components: 25 ng of human genomic DNA, 10 pmol of each
            oligonucleotide, 200 micro-M dNTPs, 0.25 U Taq  polymerase (Cetus)
            in 10 micro-l of 50 mM KCl-20 mM  Tris-HCl, pH 8.3 (at room temp),
            2.5 mM MgCl-2.  Initial denaturation at 94degC for 1.5 min, then 30
            cycles of  94degC for 15 sec, 62degC for 23 sec, and 72degC for 30
            sec, followed by a final extension at 72degC for 3.5 min, using a
            Perkin-Elmer 9600 thermocycler. PCR-amplified product size 239 bp.
            Sequence submitted by:
            Human Genome Mapping Center
            Box 0925
            University of California San Francisco
            San Francisco, CA 94143-0925  USA
            Phone: (415) 502-1612   Fax: (415) 476-8391
            e-mail: hgmcprobes@cgl.ucsf.edu
FEATURES             Location/Qualifiers
     misc_feature    31..54
                     /label=Primer1
     misc_feature    complement(245..269)
                     /label=Primer2
BASE COUNT      112 a     80 c     90 g    125 t     14 others
ORIGIN
        1 actttgaaca tcagtngctt tggcaatatt aggctgaatc aattcattac accctgatat
       61 tagttgacat cctgtgtgga tattttatgg gagggggatg gtccacaaac tgacatagtc
      121 ctgacccaga aaattctctg ggaacaagaa ttccacttgg ttccatccat tgctattttt
      181 ntcagcctca ggagtgaatg tnttgacatg tacatgcttg ttcaccctgc cagcatttac
      241 taagcaccag ctataagtta agatcacaga cataaagatg aattaagaca tctttaattt
      301 tcccnnggtg tttgatgtct agtacagagg tgacttataa gtcctgctac aagggggttt
      361 tacacaggnt ctnnaaggnt ctncatacaa ggtttggccn catacntttg ggggnggang
      421 g
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human chromosome 4 sequence-tagged site STS4-249
Date: 21 Aug 92 10:08:11 GMT
Distribution: bionet
Checksum: 11217     3

LOCUS       HUM4STS249    447 bp ds-DNA             PRI       21-AUG-1992
DEFINITION  Human chromosome 4 sequence-tagged site STS4-249
ACCESSION   L00763
KEYWORDS    STS; human chromosome 4; sequence tagged site.
SOURCE      Homo sapiens, clone p4-193 from a chromosome 4 plasmid library;
            vector pBluescript II KS + (Stratagene)
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 447)
  AUTHORS   Goold,R.D., diSibio,G., Dugaiczyk,A., Smith,K.A., Xu,H.,
            Magrane,G., Lang,D.B., Cox,D.R., Masters,S.B. and Myers,R.M.
  TITLE     Sequence-Tagged Sites for Human Chromosome 4
  JOURNAL   Unpublished (1992) see COMMENT for author address
  STANDARD  full automatic
COMMENT     PCR components: 25 ng of human genomic DNA, 10 pmol of each
            oligonucleotide, 200 micro-M dNTPs, 0.25 U Taq  polymerase (Cetus)
            in 10 micro-l of 50 mM KCl-20 mM  Tris-HCl, pH 8.3 (at room temp),
            2.5 mM MgCl-2.  Initial denaturation at 94degC for 1.5 min, then 30
            cycles of  94degC for 15 sec, 62degC for 23 sec, and 72degC for 30
            sec, followed by a final extension at 72degC for 3.5 min, using a
            Perkin-Elmer 9600 thermocycler. PCR-amplified product size 176 bp.
            Sequence submitted by:
            Human Genome Mapping Center
            Box 0925
            University of California San Francisco
            San Francisco, CA 94143-0925  USA
            Phone: (415) 502-1612   Fax: (415) 476-8391
            e-mail: hgmcprobes@cgl.ucsf.edu
FEATURES             Location/Qualifiers
     misc_feature    58..81
                     /label=Primer1
     misc_feature    complement(209..233)
                     /label=Primer2
BASE COUNT      130 a     84 c     78 g    149 t      6 others
ORIGIN
        1 gatcagagag caagatgacc tcatggttaa tgctgaatgt gctgcaagct tagcttattt
       61 ccatttccca ctctcttctt ccctgattaa tttnataaag tttataagga tggtttctaa
      121 cttacacaaa tgtcataact agaggcttgt aggaccctaa ctctgtattt cctcggtatt
      181 agttaattca gtgttcaagg caactttacg gaccataatt tcttagtaac aagaatcaac
      241 tctgtgtgtg tgtctgtgtg tgtgcatcag caacaatttt catgaaatgt aataccatta
      301 ctgtgtgaca cacattgata atttatatta tgtntctctt nnnaatgctg attgtagcag
      361 nttcaactcc ctaaattggt tttaggaccc attaataggt catgatgtgc aactggaaaa
      421 cattgagata ctccaacaca cacagga
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: T.equiperdum mRNA for 20, Bordeaux trypanozoan antigenic type 20
Date: 15 Aug 92 10:03:18 GMT
Distribution: bionet
Checksum: 63131     2

LOCUS       TRB20         758 bp ss-RNA             INV       15-AUG-1992
DEFINITION  T.equiperdum mRNA for 20, Bordeaux trypanozoan antigenic type 20
ACCESSION   X55540
KEYWORDS    antigenic variation; gene conversion; surface antigen;
            variable surface glycoprotein.
SOURCE      Trypanosoma equiperdum RNA.
  ORGANISM  Trypanosoma equiperdum
            Eukaryota; Animalia; Protozoa; Sarcomastigophora; Mastigophora;
            Kinetoplastida; Trypanosomatina; Trypanosomatidae.
REFERENCE   1  (bases 1 to 758)
  AUTHORS   Thon,G., Baltz,T., Giroud,C. and Eisen,H.
  TITLE     Trypanosome variable surface glycoproteins: composite genes and
            order of expression
  JOURNAL   Genes Dev. 9, 1374-1383 (1990)
  STANDARD  full automatic
COMMENT     From EMBL    entry TE20;  dated 05-AUG-1992.
BASE COUNT      288 a    160 c    172 g    138 t
ORIGIN
        1 acaaacgacc tagcgacatg gctcgcggac acggcgacaa aaaagcagat atccgccaca
       61 gaggtagact tcatcggcaa cctaaataca gaagccaata cggcgataaa aggattaaaa
      121 agcagcaatc cagcgccggc atgtagcaag aaaattcgcg actacaagac aatcgcagac
      181 aacagcaaat tcaatttgat ggtaacaaaa gcgcttatcg gcaaaacaga tgcagaagcg
      241 ggccaagagt caaaagaacc ggaattagcg gcagcaataa caaaatacta cggtaccgaa
      301 gggacaaaat ttgaggacca actttggaag gcaattgaaa gaacgccagc ttacctcgga
      361 gatcagaaaa aagagcaaac cacaaaaata gaaaaactag aaacactaac agaagtcggt
      421 gaagccacag ctaggggcct cgtcaagcaa ctagctgccg gagcacaagc acgacaaacg
      481 gcttctggtg acgatcaatc ggcagaaaat caatgtggtg gcaaaaagga agatgaatgc
      541 aaagatggat gcgagctggt agagggggtt tgcaaaccag taaagcaagg agagggagaa
      601 aacaaagaaa aaaccggcac cacaaacacc acaggaagca attcttttgt tattaagaag
      661 gctcctcttt ggcttgcatt tttgcttttt taactttccc ctcaattttc tttaaggaaa
      721 ttttgctact tgaaaaactt tctgatatat tttaacac
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human chromosome 4 sequence-tagged site STS4-455
Date: 21 Aug 92 10:10:35 GMT
Distribution: bionet
Checksum: 50160     3

LOCUS       HUM4STS455    439 bp ds-DNA             PRI       21-AUG-1992
DEFINITION  Human chromosome 4 sequence-tagged site STS4-455
ACCESSION   L00871
KEYWORDS    STS; human chromosome 4; sequence tagged site.
SOURCE      Homo sapiens, clone p4-500 from a chromosome 4 plasmid library;
            vector pBluescript II KS + (Stratagene)
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 439)
  AUTHORS   Goold,R.D., diSibio,G., Dugaiczyk,A., Smith,K.A., Xu,H.,
            Magrane,G., Lang,D.B., Cox,D.R., Masters,S.B. and Myers,R.M.
  TITLE     Sequence-Tagged Sites for Human Chromosome 4
  JOURNAL   Unpublished (1992) see COMMENT for author address
  STANDARD  full automatic
COMMENT     PCR components: 25 ng of human genomic DNA, 10 pmol of each
            oligonucleotide, 200 micro-M dNTPs, 0.25 U Taq  polymerase (Cetus)
            in 10 micro-l of 50 mM KCl-20 mM  Tris-HCl, pH 8.3 (at room temp),
            2.5 mM MgCl-2.  Initial denaturation at 94degC for 1.5 min, then 30
            cycles of  94degC for 15 sec, 62degC for 23 sec, and 72degC for 30
            sec, followed by a final extension at 72degC for 3.5 min, using a
            Perkin-Elmer 9600 thermocycler. PCR-amplified product size 242 bp.
            Sequence submitted by:
            Human Genome Mapping Center
            Box 0925
            University of California San Francisco
            San Francisco, CA 94143-0925  USA
            Phone: (415) 502-1612   Fax: (415) 476-8391
            e-mail: hgmcprobes@cgl.ucsf.edu
FEATURES             Location/Qualifiers
     misc_feature    5..30
                     /label=Primer1
     misc_feature    complement(223..246)
                     /label=Primer2
BASE COUNT      148 a     78 c     89 g    124 t
ORIGIN
        1 gcatccagat attcgttcta gttttatcca ccacatgaac agttggataa atcatgttaa
       61 ctctgtgagt cttattttat ctcatttgtg aaataggaat aattatttcc tgcattaaat
      121 gcatacctta tgagttcact gtgagagagg atgggaaaag atatacaaaa aaaacctgaa
      181 acactgcaca gttctgtaca aaggtagcaa aactgtcata attaacatct gcttagaagt
      241 gaggacaatg ggggctgctg ctgcaaaggc agacatgagc atgcctagtc taggtcataa
      301 tcccaataga gttgttctaa gaagaaaggt cattttgata caggaggtag aaagtcatta
      361 tttagacaga tagagggcaa aagagtcctc agcagagctt cttttcttac aaaaagcagg
      421 ccccaaatca tctctttct
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Homo sapiens DNA-binding protein (ZNF) gene, partial cds.
Date: 21 Aug 92 10:11:55 GMT
Distribution: bionet
Checksum: 59543     2

LOCUS       HUMZNFL       115 bp ds-DNA             PRI       21-AUG-1992
DEFINITION  Homo sapiens DNA-binding protein (ZNF) gene, partial cds.
ACCESSION   M88368
KEYWORDS    DNA binding protein; zinc finger protein.
SOURCE      Homo sapiens Placenta DNA.
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 115)
  AUTHORS   Bray,P.L., Lichter,P., Thiesen,H.J., Ward,D.C. and Dawid,I.B.
  TITLE     Characterization and mapping of human genes encoding zinc finger
            proteins
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 88, 9563-9567 (1991)
  STANDARD  full automatic
REFERENCE   2  (bases 1 to 115)
  AUTHORS   Lichter,P., Bray,P., Ried,T., Dawid,I.B. and Ward,D.C.
  TITLE     Clustering of C2-H2 zinc finger motif sequences within telomeric
            and fragile site regions of human chromosomes
  JOURNAL   Genomics 13, 999-1007 (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     CDS             1..115
                     /standard_name="zinc finger motif"
                     /product="DNA binding protein"
                     /gene="ZNF"
                     /note="putative"
                     /codon_start=2
                     /translation="LEKNHMNAPSRTVFTHLSSLKRHVKSHCGRKAPPGEEC"
                     /partial
BASE COUNT       35 a     28 c     26 g     26 t
ORIGIN
        1 gctggagaaa aaccatatga atgcaccaag tcggacagtc ttcacgcatc tttcttctct
       61 taaaaggcac gtcaagtctc actgtggacg aaaagcacct ccaggtgagg aatgt
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human chromosome 4 sequence-tagged site STS4-202
Date: 21 Aug 92 10:07:44 GMT
Distribution: bionet
Checksum: 03514     3

LOCUS       HUM4STS202    419 bp ds-DNA             PRI       21-AUG-1992
DEFINITION  Human chromosome 4 sequence-tagged site STS4-202
ACCESSION   L00906
KEYWORDS    STS; human chromosome 4; sequence tagged site.
SOURCE      Homo sapiens, clone C4-130 from Los Alamos National Laboratory
            chromosome 4 cosmid library, 1G4; vector SuperCos 1 (Stratagene)
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 419)
  AUTHORS   Goold,R.D., diSibio,G., Dugaiczyk,A., Smith,K.A., Xu,H.,
            Magrane,G., Lang,D.B., Cox,D.R., Masters,S.B. and Myers,R.M.
  TITLE     Sequence-Tagged Sites for Human Chromosome 4
  JOURNAL   Unpublished (1992) see COMMENT for author address
  STANDARD  full automatic
COMMENT     PCR components: 25 ng of human genomic DNA, 10 pmol of each
            oligonucleotide, 200 micro-M dNTPs, 0.25 U Taq  polymerase (Cetus)
            in 10 micro-l of 50 mM KCl-20 mM  Tris-HCl, pH 8.3 (at room temp),
            2.5 mM MgCl-2.  Initial denaturation at 94degC for 1.5 min, then 30
            cycles of  94degC for 15 sec, 62degC for 23 sec, and 72degC for 30
            sec, followed by a final extension at 72degC for 3.5 min, using a
            Perkin-Elmer 9600 thermocycler. PCR-amplified product size 284 bp.
            Sequence submitted by:
            Human Genome Mapping Center
            Box 0925
            University of California San Francisco
            San Francisco, CA 94143-0925  USA
            Phone: (415) 502-1612   Fax: (415) 476-8391
            e-mail: hgmcprobes@cgl.ucsf.edu
FEATURES             Location/Qualifiers
     misc_feature    27..49
                     /label=Primer1
     misc_feature    complement(288..310)
                     /label=Primer2
BASE COUNT       68 a    124 c     90 g    114 t     23 others
ORIGIN
        1 nntnagtgtg ggcagncagt cagggnaact ccatgtggtt actgcccatg cactctctgc
       61 tgtttttnac ctcttcttca gagtgtggtc aggntggtgg ccttgcccag cacaggaggc
      121 ccttttcctt ctgaccacct gacctgaccc acctcttagc atctgcaggc actccctgtc
      181 ccttcgctgg gccccgtggg gaactacttg cagtcatcaa attcatcatg ctgntttctt
      241 ttaattccca cacttgccaa ggtgggactg ccccgnatct ncttcccagt cgtgtgtcag
      301 aactcaggac tggacctttn cccttttccc actcctancn ctncttnaac ccgacgaacg
      361 ntttcacttn gggntcatct cttctgaggg ntggacctgn anagacggnc ctntggaat
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Odocoileus hemionus sitkensis cytochrome b gene, 3' end.
Date: 19 Aug 92 10:02:52 GMT
Distribution: bionet
Checksum: 12349     2

LOCUS       ODOAKBCB      307 bp ds-DNA             ORG       19-AUG-1992
DEFINITION  Odocoileus hemionus sitkensis cytochrome b gene, 3' end.
ACCESSION   M98485
KEYWORDS    cytochrome b.
SOURCE      Mitochondrion Odocoileus hemionus (chromosome Mitochondrion
            Odocoileus hemionus, sub_species sitkensis) adult liver DNA.
  ORGANISM  Mitochondrion Odocoileus hemionus
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Artiodactyla; Ruminantia; Pecora; Cervidae; Odocoileinae.
REFERENCE   1  (bases 1 to 307)
  AUTHORS   Carr,S.M. and Hughes,G.A.
  TITLE     The direction of hybridization between species of North American
            deer (Odocoileus) as inferred from mitochondrial cytochrome b
            sequences
  JOURNAL   J. Mammal. (1992) In press
  STANDARD  full staff_entry
FEATURES             Location/Qualifiers
     CDS             <1..298
                     /product="cytochrome b"
                     /codon_start=3
                     /translation="FGSLLGVCLILQILTGLFLAMHYTSDTMTAFSSVTHICRDVNYG
                     WIIRYMHANGASMFFICLFMHVGRGLYYGSYTFLETWNIGVILLFTVMATAFVG"
BASE COUNT       87 a     73 c     49 g     98 t
ORIGIN
        1 acttcggctc tctgctagga gtttgtttaa ttctacaaat tctaaccggc ctatttctag
       61 caatacacta tacatccgac acaataacag cattttcttc tgtcacccat atctgccgag
      121 acgtcaacta tggctgaatc attcgatata tacatgccaa tggagcatcc atattcttca
      181 tctgtctatt tatacatgtg ggacgaggac tgtactacgg atcatacact ttcctagaaa
      241 catgaaatat tggagtaatt ctcctattca cagttatagc tacagcattt gtagggtacg
      301 tcctacc
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Ossicaulis lignatilis ribosomal RNA (41LR6) large subunit, partial.
Date: 19 Aug 92 10:03:12 GMT
Distribution: bionet
Checksum: 45792     2

LOCUS       OSSRRLSB      226 bp ss-rRNA            RNA       19-AUG-1992
DEFINITION  Ossicaulis lignatilis ribosomal RNA (41LR6) large subunit, partial.
ACCESSION   M98631
KEYWORDS    large subunit ribosomal RNA.
SOURCE      Ossicaulis lignatilis (library: Duke culture collection 483) cDNA
            to rRNA.
  ORGANISM  Ossicaulis lignatilis
            Eukaryota; Fungi; Amastigomycota; Basidiomycetes.
REFERENCE   1  (bases 1 to 226)
  AUTHORS   Hibbett,D.S. and Vilgalys,R.
  TITLE     Phylogenetic relationships of the basidiomycete genus Lentinus
            inferred from molecular and morphological characters
  JOURNAL   Unpublished (1992)
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     rRNA            <1..>226
                     /note="Approximately aligns to region between positions
                     845 to 1093in Saccharomyces cerevisiae large subunit
                     nuclear rRNA."
                     /product="large subunit ribosomal RNA"
                     /citation=[1]
BASE COUNT       62 a     48 c     54 g     62 t
ORIGIN
        1 tagcgattct gacgtgcaaa tcgatcgtcg aatttggtat aggggcgaaa gctaatcgaa
       61 ccatctagta gctggttcct gccgaagttt ccctcagata gcagaaactc atttcagatt
      121 tatgtggtaa agcgaatgat tagaggcctt ggggttgcaa caaccttaac ctattctcaa
      181 actttaaata tgtaagaacg ggccgtctct tgattggacc gcccgg
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: H.sapiens IG VH mRNA (clone mAB 65VH)
Date: 14 Aug 92 10:00:49 GMT
Distribution: bionet
Checksum: 57553     3

LOCUS       HUMVHMAB65    345 bp ss-mRNA            PRI       14-AUG-1992
DEFINITION  H.sapiens IG VH mRNA (clone mAB 65VH)
ACCESSION   X54443
KEYWORDS    IgA1; VH gene family IV; autoantibody; diversity exon;
            immunoglobulin heavy chain; joining exon; monoclonal antibody;
            polyreactive; rheumatoid factor; variable region.
SOURCE      Homo sapiens RNA.
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 345)
  AUTHORS   Harindranath,N., Goldfarb,I.S., Ikematsu,H., Burastero,S.E.,
            Wilder,R.L., Notkins,A.L. and Casali,P.
  TITLE     Complete sequence of the genes encoding the VH and VL regions of
            low-high affinity monoclonal IgM and IgA1 rheumatoid factors
            produced by CD5+ B cells from a rheumatoid arthritis patient
  JOURNAL   Int. Immunol. 3, 865-875 (1991)
  STANDARD  full staff_entry
COMMENT     *source: stage=adult; *source: cell_type=B-cell heterohybridoma;
            *source: cell_line=Ab 65; *source: clone_library=lambda gt11;
            *source: clone=mAB 65 VH; *source: isolate=rheumatoid arthritis
            patient (ML); *source: chromosome=14q32; *source: is_germline=N;
            See also X54435-X54438 & X54441-47
            From EMBL    entry HSVHMAB65;  dated 29-JAN-1992.
FEATURES             Location/Qualifiers
     mat_peptide     <1..>345
                     /product="immunoglobulin heavy chain V-D-J region"
                     /gene="IGH@"
                     /codon_start=1
     CDS             <1..>345
                     /product="immunoglobulin heavy chain V-D-J region"
                     /gene="IGH@"
                     /codon_start=1
                     /translation="ETLSLTCTVSGGSISSGRYFWTWIRQHPGKGLEWIGYIYYKGDT
                     FYNPSLKSRVSISLDTSQNQFSLRLRSVTAADSAVYYCARWGYYNSNGSPLVYYFYGQ
                     NVWGPGTTVTVSS"
     misc_feature    252..253
                     /note="V-region end/D-region start"
                     /gene="IGH@"
     misc_feature    288..289
                     /note="D-region end/J-region start"
                     /gene="IGH@"
BASE COUNT       66 a    102 c     93 g     84 t
ORIGIN
        1 gagaccctgt ccctcacctg cactgtctct ggtggctcca tcagcagtgg tcgttacttc
       61 tggacctgga tccgccagca cccaggcaag gggctggagt ggattgggta catctattac
      121 aagggagaca ccttctacaa cccgtccctc aagagtcgag tttccatctc actggacacg
      181 tctcagaacc agttctccct gagactgagg tctgtgacgg ccgcggactc ggccgtgtat
      241 tattgtgcga ggtggggcta ttacaatagt aatggttctc cgctggtcta ctacttctac
      301 ggtcagaacg tctggggccc tgggaccacg gtcaccgtct cctca
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human chromosome 4 sequence-tagged site STS4-104
Date: 21 Aug 92 10:06:33 GMT
Distribution: bionet
Checksum: 57157     3

LOCUS       HUM4STS104    428 bp ds-DNA             PRI       21-AUG-1992
DEFINITION  Human chromosome 4 sequence-tagged site STS4-104
ACCESSION   M95224
KEYWORDS    STS; human chromosome 4; sequence tagged site.
SOURCE      Homo sapiens, clone C4-46 from Los Alamos National Laboratory
            chromosome 4 cosmid library, plate 4-1-180R, pos 2B; vector
            SuperCos 1 (Stratagene)
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 428)
  AUTHORS   Goold,R.D., diSibio,G., Dugaiczyk,A., Smith,K.A., Xu,H.,
            Magrane,G., Lang,D.B., Cox,D.R., Masters,S.B. and Myers,R.M.
  TITLE     Sequence-Tagged Sites for Human Chromosome 4
  JOURNAL   Unpublished (1992) see COMMENT for author address
  STANDARD  full automatic
COMMENT     PCR components: 25 ng of human genomic DNA, 10 pmol of each
            oligonucleotide, 200 micro-M dNTPs, 0.25 U Taq  polymerase (Cetus)
            in 10 micro-l of 50 mM KCl-20 mM  Tris-HCl, pH 8.3 (at room temp),
            2.5 mM MgCl-2.  Initial denaturation at 94degC for 1.5 min, then 30
            cycles of  94degC for 15 sec, 60degC for 23 sec, and 72degC for 30
            sec, followed by a final extension at 72degC for 3.5 min, using a
            Perkin-Elmer 9600 thermocycler. PCR-amplified product size 243 bp.
            Sequence submitted by:
            Human Genome Mapping Center
            Box 0925
            University of California San Francisco
            San Francisco, CA 94143-0925  USA
            Phone: (415) 502-1612   Fax: (415) 476-8391
            e-mail: hgmcprobes@cgl.ucsf.edu
FEATURES             Location/Qualifiers
     misc_feature    25..49
                     /label=Primer1
     misc_feature    complement(243..267)
                     /label=Primer2
BASE COUNT      149 a     82 c     80 g    103 t     14 others
ORIGIN
        1 agaaattcca ttccttcatn tatatccaaa aggatagaaa gcagaactca gatacctgta
       61 caccaatgtt catagcagca ttactcagag tagcccaaag gcagaaacaa cccaaatatc
      121 catcaacaga aaatttcatt tttttttaaa aagtcataca atggaatctg atgctacaac
      181 atggatgaac cttaaaagca tgtgacctga aatgagacgc aaaaggccaa acattctacg
      241 atcccactta catgagatcc cagttataca ataggcaaat tcacagagac tgaaaataga
      301 acagangtta ccagggggtg tccctggtaa acaatgagga gttactgttt natgggttca
      361 gaatttctng ttgngatgat gaaanngttc tgnnaatggc cagnggtgat ncctgctngn
      421 cactgtna
//