HDTenEightyP/NetNews huggingface dataset:
Organization: Queen's University at Kingston
Date: Fri, 8 Jan 1993 08:12:04 EST
From: <FORSDYKE@QUCDN.QueensU.CA>
Newsgroups: bionet.molbio.evolution
Subject: Re: Dosage compensation in Z/W chromosome systems
 <robison1.726451329@husc.harvard.edu>

>>    I am looking for recent work on dosage compensation in birds,
>butterflies,
>>snakes and other species with Z/W chromosome systems. The best references I
>>have are Johnson and Turner (1979) Heredity 43, 71-77 and
>>         Baverstock et al., (1982) Nature 296, 763-766.
>>    I will be checking the Science Citation Index to see who is citing these
>>papers now but there may be some papers I miss. Can anyone help?
>>                                             Sincerely,
>>                                                       Don Forsdyke
>
>Uh, could you enlighten me as to why Z/W systems need dosage compensation
>-- I thought the Z/W meant that the two chromosomes contained the
>same genes except for the sex-determination factor.

   In mammals the X is 5% of the genome and they have dosage compensation. In
birds the Z is 10% of the genome, so they would appear even more in need of
compensation. In birds the Z is like the Y chromosome, degenerate, having lost
the majority of its genes. The heterogametic sex is essentially haploid
(hemizygous) for the sex chromosomes.

               Sincerely, Don Forsdyke
From: johnest@ulrik.uio.no (John E. Stacy)
Newsgroups: bionet.molbio.evolution
Subject: mtDNA control region/D-loop polymorphisms
Date: 4 Jan 93 14:54:57 GMT
Organization: University of Oslo


Thanks to every one who shared their experiences and references 
regarding intraspecific length variation in the mitochondrial 
D-loop.  
 
In this posting I will list the references, letters I recieved 
and summarize some of the responses. 

References I recieved regarding D-loop structure, polymorphism 
and studies that exploit this polymorphism are:
*************************************************  
A.C. Wilson and co-workers: 
 
	PNAS 88:1597.  1991 
	Nature 350: 467.  1991. 
	Mol.Biol.Evol. 8:475.  1991. 

Wilkinson, G. S. and A. M. Chapman. 1991. Length and sequence variation 
in evening bat D-loop mtDNA. Genetics 128:607-617. 
[This study used PCR product lengths: the bat D-loop includes an 
81 bp seqeunce repeated between 5 and 8 times (have not found 
any mention of this in other taxa).]

Vigilant et al (Science 253(27 Sept 1991)1503-1507) 
[Comparing 610 nucleotides in 189 human individuals, they found 
substitutions at 179 sites and length changes at 22 sites. Searching 
the EMBL data base using Vigilant, the sequences used in the study are 
available.]
***************************************************

One response via e-mail reported low variation (Nancy Bowers, Penn. State). 
Her study animals are cichlid fishes from Lake Malawi, Africa. 
Bowers writes:

"There are very few fixed allelic differences at the isozyme level 
and we had hoped the D-loop region to provide some discrimanatory power.  
As it turns out, I am finding 0.3-1% intraspecific differences!
This is fairly low, even among fishes (intraspecific differences in a Lake
Tanganyika species is 5% and is 12% in an open ocean species like marlins).
...
"I am currently looking at population level variation and as expected, 
it to is very low."

Regarding PCR based methodology, Bowers writes:

"I have found the D-loop very easy to work
with and have used Tom Kocher's conserved primers for PCR."
		   ^^^^^^^^^^
>Tom Kocher's e-mail: T_Kocher@unhh.unh.edu

I seem to have lost Bowers, but here is her "normal" address:

Nancy Bowers
Intercollege Program in Ecology
Penn State Unviersity
University Park, PA  16802
**********************************************************************


In summary, at least two groups employ PCR based methodology. 
Regarding easily detectable length polymorhisms, Wilkinsin
and Chapman (see above) detect these using EtBr stained
agarose gels. The length polymorphism is due to a variable number 
of repeats of an 81 bp segment in the bat control region. I have 
seen no mention of a similar repeated segment in other animals.


Thanks again to every one who responded. 


||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
			      ||
FROM: John E. Stacy           ||  "A cup of coffee in the morning
at the University of Oslo     ||   recapitulates phylogeny"
			      ||
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
From: thomasb@SPIDER.ENTO.CSIRO.AU (Thomas Boyce)
Newsgroups: bionet.molbio.evolution
Subject: Wings and intermediate phenotypes
Date: 10 Jan 93 23:05:04 GMT
Distribution: bionet


First, my apologies to Mimi Koehl for misspelling her name, and to say
that this topic is a bit far afield from molecular evolution so I'll
keep this short.

I suggest that anyone truly interested in the issue of what 
*morphospace* is physically possible for thermoregulation and
any enhancement of insect gliding abilities should read 

Kingsolver, J. G., and M. A R. Koehl. 1985. Aerodynamics,
thermoregulation, and the evolution of insect wings: Differential
scaling and evolutionary change.  Evolution 39:488-504.

Many of Ben Jones's questions may be answered there.

Sincerely,
Thomas Boyce

thomasb@spider.ento.csiro.au
From: WERR@db1.cc.rochester.edu (JOHN WERREN)
Newsgroups: bionet.molbio.evolution
Subject: PAUP for ibm/dos
Date: 5 Jan 93 19:35:53 GMT
Organization: University of Rochester, Rochester NY
X-News-Reader: VMS NEWS 1.11

we are looking for a PAUP software program that runs on Dos/ibm
compatible machines.  We have a 386/33 system.  Swoffords old
1bm version does not accept sequence strings longer than a few
hundred bases, and we would very much like an updated version.
thanks for any help.  
 j. werren   
address  werr@db1.cc.rochester.edu
From: kristoff@NET.BIO.NET (David Kristofferson)
Newsgroups: bionet.molbio.embldatabank
Subject: BIOSCI/bionet Frequently Asked Questions
Date: 12 Jan 93 10:00:03 GMT
Distribution: bionet


New users of BIOSCI/bionet may want to read the "Frequently Asked
Questions" or "FAQ" sheets for BIOSCI.  There are two FAQ sheets for
the newsgroup system.  FAQ I describes the general purpose and uses of
the BIOSCI/bionet newsgroups.  FAQ II provides details on how to
participate in these forums.  Both of these FAQs are available for
anonymous FTP from net.bio.net [134.172.2.69] in
pub/BIOSCI/biosci1.FAQ and pub/BIOSCI/biosci2.FAQ.  They may also be
requested by sending e-mail to biosci@net.bio.net (use plain English
for your request).  The FAQs are also posted on the first of each
month to the newsgroup BIONEWS/bionet.announce immediately following
the posting of the BIOSCI information sheet.

				Sincerely,

				Dave Kristofferson
				BIOSCI/bionet Manager

				kristoff@net.bio.net
From: lipman@void.nlm.nih.gov (David Lipman)
Newsgroups: bionet.molbio.genbank
Subject: Information on the next GenBank Release
Date: 12 Jan 93 22:13:53 GMT
Organization: National Library of Medicine
X-Newsreader: Tin 1.1 PL4


Release 75.0 of NCBI-GenBank on February 15 will represent an important
step towards providing an up-to-date and internationally consistent DNA
sequence database.  As you know, GenBank consists of sequence entries
provided by author submissions to EMBL, DDBJ, and NCBI (processed by
Los Alamos National Laboratory through an interagency agreement), as
well as through journal scanning at the National Library of Medicine.
In preparing Release 75.0, NCBI will import the most recent possible
releases and updates of the EMBL and DDBJ databases (transforming all
entries into a common form, ASN.1 sequence definitions, and then into
the GenBank flatfile format).  In cases of multiple versions of records
with the same primary accession number, the record from the database
which originated and "owns" the record has been selected.  As a result
of this approach, for example, EMBL-derived records should now, on
average, be more similar to their corresponding entries in the EMBL
database.  

Two specific consequences of this approach are:

1) Locus names will be identical to that used by the source database.
This means that it is possible for different entries to have the same
locus name if derived from different databases.  This further
underscores the problem of using locus names to identify sequences.
Therefore the most reliable sequence identifier remains the accession
number.

2) There will be no segmented entries from EMBL.  
Some entries from NCBI and DDBJ indicate that they are part of a larger,
segmented sequence.  EMBL, however, always treats each segment as a
separate sequence and does not provide information that allows
automatic assembly.  Therefore, for EMBL-derived data, each individual
segment will appear as an independent entry in the GenBank release.
EMBL does provide explicit information in the feature table for coding
sequences that span several entries and these complete translations
will appear with the "/translation" feature in NCBI-GenBank Release
75.0.

Future releases will reflect additional steps to improve international
consistency and reduce redundancy.  NCBI and EMBL have begun a project
to identify and remove entries with different accession numbers but
highly redundant information.  Using information provided by PIR and
SWISS-PROT, NCBI has also begun crosslinking the DNA and protein
databases so that in future releases of Entrez:Sequences and the ASN.1
files,  DNA sequence entries will be explicitly linked to their
corresponding PIR or SWISS-PROT entries through their coding
sequences.  We will also be reaching out to interested scientists in
the biomedical community for help and suggestions in reducing
redundancy in the sequence database.


David J. Lipman
NCBI
From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human immunodeficiency virus type 1 proviral protease gene, partial
Date: 13 Jan 93 05:46:47 GMT
Distribution: bionet
Checksum: 34704 2

LOCUS       HIV310060A    297 bp ds-DNA             VRL       12-JAN-1993
DEFINITION  Human immunodeficiency virus type 1 proviral protease gene, partial
            cds.
ACCESSION   L08451
KEYWORDS    protease.
SOURCE      Human immunodeficiency virus type 1 (individual_isolate 310060)
            DNA.
  ORGANISM  Human immunodeficiency virus type 1
            Viridae; ss-RNA enveloped viruses; Positive strand RNA virus;
            Retroviridae; Lentivirinae.
REFERENCE   1  (bases 1 to 297)
  AUTHORS   Pieniazek,D., de Cock,K.M., Owen,S.M., Fridlung,C., Brattegaard,K.,
            Pieniazek,N.J., George,R.J., Schochetman,G. and Rayfield,M.A.
  TITLE     Phylogenetic relationships among Human Immunodeficiency Virus (HIV)
            isolates from single and mixed HIV-1/HIV-2 infections
  JOURNAL   Unpublished (1993)
  STANDARD  full automatic
COMMENT     *individual isolate= Cote d'Ivoire isolate from dually infected
            person.
FEATURES             Location/Qualifiers
     CDS             <1..>297
                     /evidence="EXPERIMENTAL"
                     /citation=[1]
                     /product="protease"
                     /codon_start=1
                     /translation="PQITLWQRPLVTVRIGGQLIEALLDTGADDTVLEEINLPGKWKP
                     KMIGGIGGFIKVRQYDQILIEICGKKAIGTVLVGPTPVNIIGRNMLTQIGCTLNF"
BASE COUNT      113 a     47 c     63 g     74 t
ORIGIN      
        1 cctcaaatca ctctttggca acgaccctta gtcacagtaa gaataggggg acagctaata
       61 gaagccctat tagacacagg agcagatgat acagtattag aagaaataaa tttaccagga
      121 aaatggaaac caaaaatgat agggggaatt ggaggtttta tcaaagtaag acaatatgat
      181 cagatactta tagaaatttg tggaaaaaag gccataggta ccgtattagt aggacctaca
      241 cctgtcaaca taattggacg aaatatgttg actcagattg gctgcacttt aaatttt
//
From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Mouse anti-DNA antibody kappa light chain variable region (Vk23 Vk
Date: 5 Jan 93 06:47:46 GMT
Distribution: bionet
Checksum: 19657 2

LOCUS       MUSANTDNAR    327 bp ss-mRNA            ROD       04-JAN-1993
DEFINITION  Mouse anti-DNA antibody kappa light chain variable region (Vk23 Vk
            family) mRNA.
ACCESSION   L08223
KEYWORDS    anti-DNA antibody; kappa chain; light chain; variable region.
SOURCE      Mus musculus (strain BALB/c, sub_species domesticus) cDNA to mRNA.
  ORGANISM  Mus musculus
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Rodentia; Myomorpha; Muridae; Murinae.
REFERENCE   1  (bases 1 to 327)
  AUTHORS   Gilkeson,G.S., Bloom,D.C., Pisetsky,D.S. and Clarke,S.
  TITLE     Molecular characterization of anti-DNA antibodies induced by
            immunization with bacterial DNA
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
BASE COUNT       88 a     82 c     82 g     75 t
ORIGIN      
        1 aacattgtaa tgacccaatc tcccagatcc atgtccatgt cagtaggaga gagggtcacc
       61 ttgagctgca aggccagtga gaatgtgggt cgttatgtat cctggtatca acagaaacca
      121 gagcagtctc ctaaactgct aatatacggg gcatccaacc ggtacactgg ggtccccgat
      181 cgcttcacag gcagtggatc tgcaacagat ttcactctga ccatcagcag tgtgcaggct
      241 gaagaagacc ttgcagatta tatctgtgga caaagttaca gctatcccct cacgttcggt
      301 gctggtacca agctggagct gaaacgt
//
From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: {pseudogene} Igl-V2=immunoglobulin lambda chain variable region
Date: 12 Jan 93 05:50:12 GMT
Distribution: bionet
Checksum: 35264 2

LOCUS       S117316       508 bp    mRNA            ROD       11-JAN-1993
DEFINITION  {pseudogene} Igl-V2=immunoglobulin lambda chain variable region
            subfamily V2 {clone RLV29, rearranged V region} [Rattus
            norvegicus=laboratory rats, DA, spleen, mRNA Partial, 508 nt]
ACCESSION   S48180
KEYWORDS    .
SOURCE      laboratory rats DA spleen
  ORGANISM  Rattus norvegicus
            Unclassified.
REFERENCE   1  (bases 1 to 508)
  AUTHORS   Aguilar,B.A. and Gutman,G.A.
  TITLE     Transcription and diversity of immunoglobulin lambda chain variable
            genes in the rat.
  JOURNAL   Immunogenetics 37, 39-48 (1992)
  STANDARD  full automatic
COMMENT     This entry [NCBI gibbsq 117316] was created by the journal scanning
            component of NCBI/GenBank at the National Library of Medicine.
            one-base deletion in leader region puts remaining sequence
            out-of-frame.
            This sequence comes from Fig. 3.
FEATURES             Location/Qualifiers
     misc_feature    1..508
                     /partial
                     /note="This feature applies to a gene which lacks a coding
                     region feature. Description: immunoglobulin lambda chain
                     variable region subfamily V2"
                     /gene="Igl-V2"
BASE COUNT      127 a    103 c    108 g    170 t
ORIGIN      
        1 aaaatctgag tgtttcacca tgacatggac tctactattc cttgccttcc ttcatcactt
       61 aacagatgga tgtgtttaag aaattattgg actgtaattg tttgttgctt ggaatgtttg
      121 gcccctagtt tttactgtgg atggaatgac cttgttctaa ctgattttta ttattttctt
      181 cttatagggt catgtgccca gtttgtgctt actcagccaa actctgtgtc tacgtctcta
      241 ggaagcacag tcaaactgtc ttgcaagtgc agcactggta acattggaag ctactatgtg
      301 cactggtacc agcagcatga gggaagatct cccaccacta tgatttttga gtatgataag
      361 agaccagatg gagttcctga tagggtctct ggctccattg acagatcttc caactcagcc
      421 ttcctgacaa tcaataatgt gcagactgaa gatgaagctg actacttctg tcattcttat
      481 gatagtagta tattttcggc ggtggaac
//
From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: steroidogenic factor 1 [cattle, adrenal glands, mRNA Partial, 201
Date: 7 Jan 93 05:53:40 GMT
Distribution: bionet
Checksum: 51316 2

LOCUS       S115167       201 bp    mRNA            MAM       06-JAN-1993
DEFINITION  steroidogenic factor 1 [cattle, adrenal glands, mRNA Partial, 201
            nt]
ACCESSION   S45997
KEYWORDS    .
SOURCE      cattle adrenal glands
  ORGANISM  Unclassified.
            Unclassified.
REFERENCE   1  (bases 1 to 201)
  AUTHORS   Lala,D.S., Rice,D.A. and Parker,K.L.
  TITLE     Steroidogenic factor I, a key regulator of steroidogenic enzyme
            expression, is the mouse homolog of fushi tarazu-factor I.
  JOURNAL   Mol. Endocrinol. 6, 1249-1258 (1992)
  STANDARD  full automatic
COMMENT     This entry [NCBI gibbsq 115167] was created by the journal scanning
            component of NCBI/GenBank at the National Library of Medicine.
            This sequence comes from Fig. 8.
FEATURES             Location/Qualifiers
     CDS             1..201
                     /partial
                     /note="mismatches(37-9, 41-9, 51).  SF-1;  For the protein
                     sequence (NCBI gibbsq 115168):  Method: conceptual
                     translation supplied by author.  This sequence comes from
                     Fig. 8.
                     Author-given protein sequence is in conflict with the
                     conceptual translation"
                     /product="steroidogenic factor 1"
                     /codon_start=1
                     /translation="CPVCGDKVSGYHYGLLTCESCKGFFKRTVQNNKHYTESQSCKID
                     KTQRKRCPFCRFQKCLTVGMRLE"
BASE COUNT       49 a     56 c     60 g     36 t
ORIGIN      
        1 tgtccagtgt gtggtgacaa ggtgtcgggc taccactacg ggctgctcac gtgcgagagc
       61 tgcaagggct tcttcaagcg cacagtccag aacaacaagc attacacgtg caccgagagt
      121 cagagctgca aaatcgacaa gacgcagcgt aagcccttct gccgcttcca gaagtgcctg
      181 acggtgggca tgcgcctgga a
//
From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: granulin [human, Genomic, 251 nt, segment 11 of 12]
Date: 6 Jan 93 06:57:12 GMT
Distribution: bionet
Checksum: 25257 2

LOCUS       M248858S11    251 bp    DNA             PRI       08-DEC-1992
DEFINITION  granulin [human, Genomic, 251 nt, segment 11 of 12] 
ACCESSION   S46618
KEYWORDS    .
SEGMENT     11 of 12
SOURCE      human
  ORGANISM  Unclassified.
            Unclassified.
REFERENCE   1  (bases 1 to 251)
  AUTHORS   Bhandari,V. and Bateman,A.
  TITLE     Structure and chromosomal location of the human granulin gene.
  JOURNAL   Biochem. Biophys. Res. Commun. 188, 57-63 (1992)
  STANDARD  full automatic
COMMENT     This entry [NCBI gibbsq 116086] was created by the journal scanning
            component of NCBI/GenBank at the National Library of Medicine.
            This sequence comes from Fig. 2.  Map location: 17.
BASE COUNT       45 a     81 c     79 g     46 t
ORIGIN      
        1 ttcccgccag gctgtgtgct gcgaggatcg ccagcactgc tgcccggctg gctacacctg
       61 caacgtgaag gctcgatcct gcgagaagga agtggtctct gcccagcctg ccaccttcct
      121 ggcccgtagc cctcacgtgg gtgtgaagga cgtggagtgt ggggaaggac acttctgcca
      181 tgataaccag acctgctgcc gagacaaccg acagggctgg gcctgctgtc cctaccgcca
      241 ggtcagtgcc a
//
From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Homo sapiens (clone 14A-15mer(55)) alpha-satellite repeat.
Date: 13 Jan 93 05:48:40 GMT
Distribution: bionet
Checksum: 14861 3

LOCUS       HUMSATEE      550 bp ds-DNA             PRI       12-JAN-1993
DEFINITION  Homo sapiens (clone 14A-15mer(55)) alpha-satellite repeat.
ACCESSION   L08510
KEYWORDS    satellite repeat.
SOURCE      Homo sapiens DNA.
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 550)
  AUTHORS   Warburton,P.E. and Willard,H.F.
  TITLE     PCR amplification of tandemly repeated DNA: Analysis of intra- and
            interchromosomal sequence variation and homologous unequal
            crossing-over in human alpha satellite DNA
  JOURNAL   Nucleic Acids Res. (1992) In press
  STANDARD  full automatic
REFERENCE   2  (bases 1 to 550)
  AUTHORS   Warburton,P.E., Waye,J.S. and Willard,H.F.
  TITLE     Unequal crossing-over in tandemly repeated DNA: Implications for
            higher-order structural characteristics within centromeric
            heterochromatin
  JOURNAL   Unpublished (1993)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     repeat_region   1..550
                     /rpt_family="satellite repeat"
                     /citation=[1]
                     /note="'chromosome 17 alpha satellite 15 monomer
                     higher-order repeat,portion sequenced between PCR primer
                     17-12E2 and 17-1A'"
     misc_recomb     63..163
                     /citation=[2]
                     /note="'window of recombination between two chromosome
                     16mers, deletes onemonomer'"
BASE COUNT      160 a    106 c    117 g    167 t
ORIGIN      
        1 ttcctttgga tggagcagtt tcgaaacaca ctatttgtag aatgtgcaag tggatattgg
       61 ggcctctctg aggatttcgt tggaaacggg ataaaccgca cagaactaaa cagaagcatt
      121 ctcagtacct tcttcgtgat gtttgcattc aactcacagt gctgaacctt tctttgatag
      181 ttcagctttg aaacactctt tttgtagaaa ctgcaagtgg atatttggtc ctctgtgagg
      241 atttcgttgg aaaagggata aaccgcacag aactaaacag aagcattctc agaaccttct
      301 tcgtgatgtc tgcattcaac tcacagtgtg gaacctttct ttgatagttc aggtttgaaa
      361 cactcttttt gtagaaactg caaggggata attgcacttc tttgaggcct accgtagtaa
      421 aggaaataac ttcctataaa aacaagacag aagctttctc agaaaattct ttgggatgat
      481 tgagttgaac tcacagagct ggacattcct tgcgatgtag cagtttagaa acacactttc
      541 tgcagaatct
//
From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human STS primer pGSE8 for locus D21S15 (21q22.3), reverse.
Date: 7 Jan 93 05:51:24 GMT
Distribution: bionet
Checksum: 46756 2

LOCUS       HUMSTS21TR     19 bp ds-DNA             PRI       06-JAN-1993
DEFINITION  Human STS primer pGSE8 for locus D21S15 (21q22.3), reverse.
ACCESSION   M96007
KEYWORDS    STS sequence; primer; sequence tagged site.
SOURCE      Homo sapiens (library: PUC9) DNA.
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 19)
  AUTHORS   Tang,X., Tashiro,H., Eki,T., Murakami,Y., Soeda,E., Sakakura,T.,
            Watkins,P.C. and Yokoyama,K.
  TITLE     Generation of nineteen STS markers that can be anchored at specific
            sites on human chromosome 21
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
COMMENT     PCR Profile:
            Denaturation: 94 C for 1 min
            Annealing: 55 C for 2 min
            Polymerization: 72 C for 3 min
            PCR cycles: 35.
BASE COUNT        5 a      4 c      5 g      5 t
ORIGIN      
        1 gcacagtgtt aagatctgc
//
From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Hepatitis C virus (HCV) gene for NS5, partial sequence.
Date: 9 Jan 93 05:45:58 GMT
Distribution: bionet
Checksum: 41661 2

LOCUS       HPCB10        157 bp ss-RNA             VRL       09-JAN-1993
DEFINITION  Hepatitis C virus (HCV) gene for NS5, partial sequence.
ACCESSION   D10559 D90515
KEYWORDS    NS5.
SOURCE      Hepatitis C virus from human plasma of patient 10 (library: lambda
            gt11) cDNA to genomic RNA.
  ORGANISM  Hepatitis C virus
            Viridae; ss-RNA enveloped viruses; Positive strand RNA virus;
            Togaviridae incertae sedis.
REFERENCE   1  (bases 1 to 157)
  AUTHORS   Kato,N., Ootsuyama,Y., Ohkoshi,S., Nakazawa,T., Mori,S.,
            Hijikata,M. and Shimotohno,K.
  TITLE     Distribution of plural HCV types in Japan
  JOURNAL   Biochem. Biophys. Res. Commun. 181, 279-285 (1991)
  STANDARD  full automatic
COMMENT     Submitted (30-Sep-1991) to DDBJ by: Nobuyuki Kato National Cancer
            Center Research Institute Virology Division 5-1-1 Tsukiji Chou-ku
            Tokyo 104 Japan Phone:  03-3542-2511 x4701 Fax:    03-3545-3567.
FEATURES             Location/Qualifiers
     CDS             <1..>157
                     /gene="HCV-B10"
                     /product="NS5"
                     /codon_start=2
                     /translation="EESIYQACSLPEEARTAIHALTERLYVGGPMFNSKGQTCGYRRC
                     RASGVLTT"
BASE COUNT       31 a     49 c     50 g     27 t
ORIGIN      
        1 tgaggagtcc atttaccagg cctgctccct gcccgaggag gcccgcactg ccatacacgc
       61 gctgactgag agactttacg tgggggggcc catgttcaac agcaagggcc aaacctgcgg
      121 gtacaggcgt tgccgcgcaa gcggggtgct taccact
//