From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Transposon Tn4551 streptomycin adenyltransferase (aadS) gene,
Date: 10 Sep 92 10:36:21 GMT
Distribution: bionet
Checksum: 10711     5

LOCUS       TRNAADSA     2256 bp ds-DNA             BCT       10-SEP-1992
DEFINITION  Transposon Tn4551 streptomycin adenyltransferase (aadS) gene,
            complete cds.
ACCESSION   M72415
KEYWORDS    streptomycin adenyltransferase; streptomycin resistance.
SOURCE      Transposon Tn4551 DNA.
  ORGANISM  Transposon Tn4551
            Prokaryotae.
REFERENCE   1  (bases 1 to 2256)
  AUTHORS   Smith,C.J., Owen,C. and Kirby,L.
  TITLE     Activation of a cryptic streptomycin resistance gene in the
            Bacteroides erm transposon Tn4551
  JOURNAL   Mol. Microbiol. 6, 2287-2297 (1992)
  STANDARD  full staff_entry
FEATURES             Location/Qualifiers
     CDS             complement(147..995)
                     /note="ORF-2 with homology to Tn21 tnpI gene"
                     /note="putative"
                     /codon_start=1
                     /translation="MILIERRISTKINIELFCIYECFVIFATHTYNRSMDVDRLILEF
                     ERKLVLQRYSANSIANYKSSARSFLDLAKRKFSHPAEIDELAVEKYVLWKIERHRIGS
                     SHQRMIVASIDKFYKSIYGRELKIKHLYPSRRTFALPNCLNIDEVKRIIDGTENIKHR
                     CMIKMLYGSGLRLSELINLEIADIDSARMLIRIVKSKGNKDRVVTLPESILTDLRTCY
                     ILHKPDVYLFEGQWGGPYSAKSVQMVVKNAASRAGIRKRVTPGCVLNIFQLPTSTSSG
                     HPSQMS"
     CDS             1121..1984
                     /function="streptomycin resistance"
                     /note="cryptic gene that is activated by unknown
                     mechanism"
                     /product="streptomycin adenyltransferase"
                     /gene="aadS"
                     /evidence=EXPERIMENTAL
                     /codon_start=1
                     /translation="MKVREEKLRTIIEWSEKNEDVRVLLLTSSLVNPLALVDEFSDLD
                     IEFVFEDNTNYISDKSWTLKFGNPIAMIEEDESCFNHKHAMKMLLYEDGVKVDFKLYS
                     KSKFIKETQEKELPEDWDIGYKILIDKDGITKQMLKPTYQISIIKKPSEKEFQNLIND
                     FWWDTTYVAKCLVRDEIFYAKFMSETVIRTEYLIPLIEWHIASEHNWNITTNKYGRLF
                     KKYLNQEMWAKTEQTFSGSDIKENWTALFSMTDLVSEIGTELSKKLEYKYPDKLENDI
                     RKYLAGLKPKT"
                     /citation=[1]
BASE COUNT      730 a    443 c    438 g    645 t
ORIGIN
        1 ggatccgttc atcggaattg ccgaatccat cggttatttc aggggcgtgg tagccacaac
       61 gagcgacaga caaaaataaa agataatttc attctctctt tatctcgaaa gggtttgtgt
      121 tccgatgcga atgcaatccc ttttttttat gacatttgac tcggatgccc acttgaggtg
      181 gaggtcggga gttgaaaaat attcagaacg catccaggcg tgactctctt cctgattccg
      241 gctcttgacg cggcgttctt gacaaccatc tgcacggact ttgccgaata tgggccgccc
      301 cactgccctt caaacagata tacatcgggc ttatgcagga tgtaacaggt gcgaagatca
      361 gtcagaatgg attccgggag cgtcacaacc ctgtctttgt tccccttgga cttgacgatg
      421 cggatcagca tcctggcaga atcaatatcg gctatctcca gattgatcag ttcgctcaat
      481 cgcagccccg atccataaag cattttgatc atgcaacggt gcttgatgtt ttcggtgccg
      541 tcgatgatcc ttttcacttc atctatattc agacaattgg gcaacgcaaa ggtcctcctc
      601 gacggataaa ggtgttttat ctttaactcc ctgccataaa tcgacttgta gaacttgtcg
      661 atcgacgcca ctatcatgcg ctggtgagaa ctgccgatcc tgtgcctctc gattttccac
      721 agcacatatt tctcaaccgc caactcgtcg atttcagccg gatgcgagaa tttccttttc
      781 gcaagatcaa ggaagctcct cgcgctgctt ttgtagttag cgatcgagtt cgcactgtag
      841 cgctgcaaga cgagcttccg ctcaaattca agtatcaatc tgtcaacatc catgctccgg
      901 ttataagtgt gagtggcaaa gataacaaaa cattcgtaaa tgcaaaataa ttctatgttt
      961 attttcgtgc ttatccttct ctctatcaat atcatatcaa ggagttccac ttttactcac
     1021 aaatcggttt acgaatgtta tatggtttgt atttacgaat gttgtatatt tgttgcgtaa
     1081 ataagacgtt gtacgacata gtaaaaccga accaaacaaa atgaaagtca gagaagaaaa
     1141 gttaagaaca attatagaat ggtcggagaa aaacgaagat gtaagagttc ttcttctgac
     1201 aagttcactt gtaaatcctt tagcacttgt tgacgaattt agtgatttag acattgaatt
     1261 tgtttttgag gataatacaa attacatttc agacaaaagc tggacgctta aattcggaaa
     1321 tccaattgct atgattgaag aagacgaaag ttgttttaac cataaacacg caatgaaaat
     1381 gctactttat gaagacggtg tgaaagtaga ttttaaactt tacagcaaat caaaatttat
     1441 aaaggaaacg caagagaaag aattaccaga agattgggat attggctata aaattttaat
     1501 tgataaagat ggtattacaa agcaaatgct gaaaccaact tatcaaattt ccattatcaa
     1561 aaaaccgtct gaaaaagagt ttcaaaatct aataaacgat ttttggtggg acacaactta
     1621 cgtggcaaag tgtcttgtga gagatgaaat attctatgcg aaatttatgt cggaaaccgt
     1681 tattcgcaca gaatatttaa ttcctttaat tgaatggcac attgcaagtg aacacaattg
     1741 gaacataacg accaataaat atggacggct tttcaaaaag tatcttaacc aggaaatgtg
     1801 ggctaaaaca gaacaaacat tttcagggag cgatataaag gaaaattgga ctgctctatt
     1861 ttcaatgact gatttagttt cagaaatagg aactgaattg tcaaaaaaat tagagtacaa
     1921 atacccggat aaattagaaa atgacatacg aaaatattta gctggactaa aacccaaaac
     1981 ataactacgt cgtacaacat cgtattggca ataggcgggc tgaacggctt cgtatcaacg
     2041 gaagtgcaaa attcaacttc tgttcttcga ttaaagttca gtgctaaaaa tcccgcccat
     2101 cgccaatacg cggcacgtta cctgcaagca taaaaaacca aaccgtaaat tcaagaaact
     2161 aatgaaatca aaaagtttat tttcttttat tgtaacactt ttccttattt atggatgttc
     2221 gtctaacaga caagcagatg gaaaatcaaa tatttt
//