From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: S.cerevisiae gene for mitochondrial release factor 1
Date: 3 Jan 93 22:09:38 GMT
Distribution: bionet
Checksum: 29580 6

LOCUS       YSCMRF1      2600 bp ds-DNA             PLN       21-DEC-1992
DEFINITION  S.cerevisiae gene for mitochondrial release factor 1
ACCESSION   X60381
KEYWORDS    MRF1; mitochondrial release factor 1.
SOURCE      Saccharomyces cerevisiae DNA.
  ORGANISM  Saccharomyces cerevisiae
            Eukaryota; Fungi; Eumycota; Hemiascomycetes; Endomycetales;
            Saccharomycetaceae.
REFERENCE   1  (bases 1 to 2600)
  AUTHORS   Pel,H.J., Maat,M.J., Rep,M. and Grivell,L.A.
  TITLE     The yeast nuclear gene MRF1 encodes a mitochondrial peptide chain
            release factor and cures several mitochondrial RNA splicing defects
  JOURNAL   Nucleic Acids Res. 20, 6339-6346 (1992)
  STANDARD  full automatic
COMMENT     *source: clone_library=YEp13 (ref.Nasmyth et al. 1980, Cell 19,
            753-764);
               From EMBL    entry SCMRF1;  dated 07-DEC-1992.
FEATURES             Location/Qualifiers
     CDS             <1..839
                     /note="ORF2"
                     /codon_start=3
                     /translation="ALPIVLGASIPAFAFGLFFPLSKRSFPKKYLNPFFQVKLTILLN
                     LLVYSTLPHKEFRFIFPLQPLFILISSFGLLRLDRDYWKRLSGLKSLLWLVPFVSVFI
                     ALLLDTFHESGSIEVMKFLHEEPEIDSLGFIMPCHSTPGQSYLHRSDIQDLWSITCNP
                     PLHLLGDPEAYSKLETYMDESDHLYDDISAFIYKNFPPPFRKDLRSPGKTYSHEWPTY
                     LVVFEHMENAFLKDFLKDSSYIEYNRFFNSLAHWDSRRSGDIIIYYKLPFDYSDIPAA
                     DI"
     CDS             1150..2391
                     /product="mitochondrial release factor 1"
                     /codon_start=1
                     /translation="MWLSKFQFPSRSIFKGVFLGHKLPLLVRLTSTTTNSKSNGSIPT
                     QYTELSPLLVKQAEKYEAELKDLDKDLSCGIHFDVNKQKHYAKLSALTDTFIEYKEKL
                     NELKSLQEMIVSDPSLRAEAEQEYAELVPQYETTSSRLVNKLLPPHPFADKPSLLELR
                     PGVGGIEAMIFTQNLLDMYIGYANYRKWKYRIISKNENESGSGIIDAILSIEEAGSYD
                     RLRFEAGVHRVQRIPSTETKGRTHTSTAAVVVLPQIGDESAKSIDAYERTFKPGEIRV
                     DIMRASGKGGQHVNTTDSAVRLTHIPSGIVVSMQDERSQHKNKAKAFTILRARLAEKE
                     RLEKEEKERKARKSQVSSTNRSDKIRTYNFPQNRITDHRCGFTLLDLPGVLSGERLDE
                     VIEAMSKYDSTERAKELLESN"
BASE COUNT      823 a    490 c    521 g    766 t
ORIGIN      
        1 aagctttacc tatcgtatta ggtgcaagta ttcccgcttt tgcatttgga ttattttttc
       61 cactaagcaa aaggagtttc ccaaagaagt atttaaaccc gtttttccaa gtgaaactca
      121 ctatcttgtt gaatctgttg gtgtactcta cgttacctca taaggaattt aggttcatct
      181 tcccattaca gccacttttc atattaatat catcatttgg ccttttgaga cttgatagag
      241 attattggaa aagactatct ggcctaaaat ctttactctg gcttgttcca ttcgtgtcgg
      301 tgttcatcgc attacttttg gacacattcc acgaatctgg atctatcgaa gttatgaaat
      361 ttttacatga agagccagaa atagatagtt tgggattcat aatgccctgt cattcgacac
      421 ctggtcaaag ctatttacac cgcagtgaca ttcaagatct gtggtcgatt acttgtaacc
      481 ctccattgca tttacttgga gacccagagg cttactccaa actagagacc tacatggatg
      541 aaagtgacca tttgtatgac gatatatcgg cattcattta caaaaatttc ccaccgcctt
      601 ttcgtaagga tctaaggagt ccaggtaaaa catacagcca cgaatggcca acatatttgg
      661 ttgttttcga gcacatggaa aacgcatttc taaaggattt cctgaaagat tcatcataca
      721 ttgaatacaa tagattcttc aactctcttg cgcactggga ttcaagaaga tcaggagaca
      781 taataattta ttataaatta ccattcgatt atagcgatat tccagcggca gatatttaaa
      841 tttgtgtatt cttcacttat cagtttactt catttcgcga aaaagccttt aattattagt
      901 gatttattta ctacatgata ttaatatatg tgtataatta tgcaataagg cctctcagaa
      961 aatatatgtg ttacttctcg ttgtgccatc tgtactaggc tgagtaattg tgtatttcat
     1021 atacttgtta ggaggatttg ctcgctcgga ggataaatac agagcgttaa tagtgcgtat
     1081 catatgtttt ataatatctc aggaatgaaa agttcgtaga gcagaacaat atatttcatt
     1141 ggtgcaagca tgtggctttc aaagttccag ttcccctcga ggtccatctt taagggtgtt
     1201 tttttgggtc acaagttgcc gttactagtg cgactgacat caacaacgac taactctaag
     1261 agcaacggca gcatcccaac acaatacaca gaactctctc cgttacttgt taagcaagcg
     1321 gaaaagtatg aagctgaact aaaagatctt gacaaagacc tttcttgcgg cattcatttt
     1381 gacgtgaata agcagaaaca ttatgctaaa ttatcagcgc tcactgatac atttattgag
     1441 tataaggaaa aactaaatga attgaaaagt ttacaagaaa tgattgtatc tgatccatca
     1501 ttaagggcgg aggctgaaca agaatatgca gaactggtcc cccaatatga aacgacctcc
     1561 tcaagattgg taaataaact tcttccaccg catccgttcg cagataaacc tagcttacta
     1621 gagcttcgac cgggtgtagg tggcattgaa gccatgattt ttacccagaa tctattggat
     1681 atgtatattg gctatgccaa ctatagaaaa tggaagtatc gaattatatc gaaaaatgaa
     1741 aacgaaagtg ggtcaggcat tatcgatgcc attctgagca ttgaagaagc cggatcttac
     1801 gatcgtttga ggtttgaagc aggcgttcac agggtgcaga gaattcctag tacggagact
     1861 aaaggaagga cgcacacatc cactgctgcc gtggttgtgt tacctcaaat tggtgacgaa
     1921 tctgctaaat ctattgacgc ttatgaaaga acatttaaac ctggtgaaat cagagttgac
     1981 atcatgcgtg caagcgggaa aggtggtcaa catgtgaata cgacagactc tgctgtcaga
     2041 ttaacccata tcccctctgg aattgttgtt tccatgcaag atgaaagatc tcaacacaag
     2101 aataaggcta aagcatttac gatcttaaga gcgagacttg cagagaagga aaggctggaa
     2161 aaggaggaaa aagaaagaaa agcaagaaag agtcaggttt ctagcaccaa taggtctgat
     2221 aaaattagaa catacaattt tccacagaac agaatcactg atcataggtg cgggttcaca
     2281 ttgttggacc tgccaggtgt gttatctgga gaacgattgg acgaagttat tgaggcaatg
     2341 tctaaatatg atagcaccga acgggcaaaa gaattattgg agagtaactg acgaacgtaa
     2401 cttttcgttt cttgtaaata tcatatcgta tctatcactt ctgtaaatct tcttttctat
     2461 tgttctgcgc cacgtaagaa ctgacgcaca tagtagatgc ccgaaatatg caaaactctg
     2521 tgaaataaat tgtaccgcct caacagttca gtaagaatag tacacaaaga gagtgaagta
     2581 cgaaccgtgt tttgctcaag
//