From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Shigella dysenteriae O-antigen polysaccharide biosynthesis rfbX,
Date: 28 Dec 92 21:45:54 GMT
Distribution: bionet
Checksum: 57469 12

LOCUS       SHFRFBCLUS   5387 bp ds-DNA             BCT       02-DEC-1992
DEFINITION  Shigella dysenteriae O-antigen polysaccharide biosynthesis rfbX,
            O-antigen polymerase (rfc), rhamnosyl tranferase I and II (rfbR and
            rfbQ) and rfbD genes, complete cds.
ACCESSION   L07293
KEYWORDS    O-antigen polymerase; dTDP-dehydrorhamnose reductase; rfbD gene;
            rfbQ gene; rfbR gene; rfbX gene; rfc gene; rhamnosyl transferase I;
            rhamnosyl transferase II.
SOURCE      Shigella dysenteriae DNA.
  ORGANISM  Shigella dysenteriae
            Prokaryotae; Gram-negative facultatively anaerobic rods;
            Enterobacteriaceae.
REFERENCE   1  (sites)
  AUTHORS   Sturm,S., Jann,B., Jann,K., Fortnagel,P. and Timmis,K.N.
  TITLE     Genetic and biochemical analysis of Shigella dysenteriae 1 O
            antigen polysaccharide biosynthesis in Escherichia coli K-12:
            structure and functions of the rfb cluster
  JOURNAL   Microb. Pathog. 1, 307-324 (1986)
  STANDARD  full automatic
REFERENCE   2  (bases 1 to 5387)
  AUTHORS   Klena,J.D. and Schnaitman,C.A.
  TITLE     Function of the rfb gene cluster and rfe gene in the synthesis of O
            andtigen by Shigella dysenteriae 1
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     CDS             <1..324
                     /standard_name="RFBD"
                     /gene="rfbD"
                     /note="putative"
                     /product="dTDP-6-deoxy-L-lyxo-4 hexulose 4-reductase"
                     /codon_start=1
                     /translation="KVFEFSSLIFVNRHPTFGKWIGVNLSAEIKRQLWIPEGFAHGFL
                     VLSETAEFVYKTTNYYNPSFEKSISYSDPTIKIQWPNLQDMHVKLSNKDLNAKNFFNN
                     NSLMQ"
     CDS             321..1511
                     /standard_name="RFBX"
                     /gene="rfbX"
                     /evidence="EXPERIMENTAL"
                     /codon_start=1
                     /translation="MKKNILLLFLVHGANYLFPFIVLPYQTRILSIETFADVAKIQAA
                     VMLLSLIVNYGYNLSSTRAIARAVSQAEINKIYSETLIVKLLLATICLALGCVHLMYV
                     KEYSLIYPFIISSIYLYGSALFATWLFQGLEKMKAVVIATTIAKLTGVILTFILVKSP
                     NDIVAALFTQNIGMFISGIISIYLVRKNKYATVICFRLKNIIVSLKEAWPFFLSLAAT
                     SVYTYFNVILLSFYAGDYVVANFNAADKLRMAAQGLLIPIGQAVFPRLSKLEGYEYSS
                     KLKIYAIRYAIFGVCISAGLVFLGPMLTTIYLGKEYSLSGEYLQSMFLLPATISISTI
                     LSQWMLIPQGKEKILSRIYILGAIVHLLYAFPLVYYYGAWGMVISILFTEVLIVLFML
                     KAVK"
     CDS             1508..2650
                     /standard_name="RFC"
                     /gene="rfc"
                     /evidence="EXPERIMENTAL"
                     /product="O-antigen polymerase"
                     /codon_start=1
                     /translation="MTYFTGFILILFAIIIKRLTPSQSKKNIVLIANAFWGILLVGYT
                     FNEQYFVPLSATTLFFILAFLFFFSMTYILIARSGRVVFSFGTGFIESKYIYWFAGMI
                     NIISICFGIILLYNNHFSLKVMREGILDGSISGFGLGISLPLSFCCMYLARHENKKNY
                     FYCFTLLSFLLAVLSTSKIFLILFLVYIVGINSYVSKKKLLIYGVFVFGLFALSSIIL
                     GKFSSDPEGKIISAIFDTLRVYLFSGLAAFNLYVEKNATLPENLLLYPFKEVWGTTKD
                     IPKTDILPWINIGVWDTNVYTAFAPWYQSLGLYAAIIIGILLGFYYGIWFSFRQNLAV
                     GFYQTFLCFPLLMLFFQEHYLLSWKMHFIYFLCAILLAMRKALEYE"
     CDS             2643..3491
                     /standard_name="RFBR"
                     /gene="rfbR"
                     /evidence="EXPERIMENTAL"
                     /product="rhamnosyl transferase II"
                     /codon_start=1
                     /translation="MNKYCILVLFNPDISVFIDNVKKILSLDVSLFVYDNSANKHAFL
                     ALSSQEQTKINYFSICENIGLSKAYNETLRHILEFNKNVKNKSINDSVLFLDQDSEVD
                     LNSINILFETISAAESNVMIVAGNPIRRDGLPYIDYPHTVNNVKFVISSYAVYRLDAF
                     RNIGLFQEDFFIDHIDSDFCSRLIKSNYQILLRKDAFFYQPIGIKPFNLCGRYLFPIP
                     SQHRTYFQIRNAFLSYRRNGVTFNFLFREIVNRLIMSIFSGLNEKDLLKRLHLYLKGI
                     KDGLKM"
     CDS             3516..4427
                     /standard_name="RFBQ"
                     /gene="rfbQ"
                     /evidence="EXPERIMENTAL"
                     /product="rhamnosyl transferase I"
                     /codon_start=1
                     /translation="MIKKKVAAIIITYNPDLTILRESYTSLYKQVDKIILIDNNSTNY
                     QELKKLFEKKEKIKIVPLSDNIGLAAAQNLGLNLAIKNNYTYAILFDQDSVLQDNGIN
                     SFFFEFEKLVSEEKLNIVAIGPSFFDEKTGRRFRPTKFIGPFLYPFRKITTKNPLTEV
                     DFLIASGCFIKLECIKSAGMMTESLFIDYIDVEWSYRMRSYGYKLYIHNDIHMSHLVG
                     ESRVNLGLKTISLHGPLRRYYLFRNYISILKVRYIPLGYKIREGFFNIGRFLVSMIIT
                     KNRKTLILYTIKAIKDGINNEMGKYKG"
BASE COUNT     1771 a    714 c    969 g   1933 t
ORIGIN      
        1 aaagtttttg aatttagcag tttgatattc gtaaatcgtc accctacatt tgggaaatgg
       61 attggggtga atttgtctgc tgagattaag cgtcagttgt ggatacctga aggatttgcg
      121 catggatttt tggtgctgag tgaaacggct gagtttgttt ataaaacaac aaactattac
      181 aatccaagtt ttgaaaaaag tatttcatac tcagatccta ccattaaaat tcagtggccc
      241 aatttacagg atatgcatgt taaattatca aataaggatt tgaatgctaa gaactttttt
      301 aataacaata gtttaatgca atgaagaaaa atatattgct cttgttctta gtacatgggg
      361 caaattattt gttcccgttt atagttcttc catatcaaac tcgaatatta agcatcgaga
      421 cattcgcaga tgtagcaaaa attcaagccg ctgtgatgct tttatcttta atcgtaaatt
      481 atggatataa cttatcaagt acaagagcta tagctagggc cgtatctcaa gcagaaataa
      541 ataagatcta tagtgagact cttattgtaa aattattatt ggcaaccatt tgtcttgcac
      601 ttggttgcgt acatttgatg tatgtcaaag agtactcatt gatatatcct tttataatca
      661 gttcgatata tctttatggt agtgcattat ttgctacttg gttattccaa ggacttgaga
      721 aaatgaaagc ggtcgttata gcaacaacaa tcgctaaact gactggtgtg atacttactt
      781 ttattttagt taagtctcca aatgatatag ttgcagctct ttttacacaa aacattggga
      841 tgtttataag tggtataata tctatttatt tggtaaggaa aaacaaatat gcaaccgtaa
      901 tatgttttcg acttaaaaat attattgtaa gcttaaaaga agcgtggccg ttttttttat
      961 cattagctgc aacaagtgta tatacatatt ttaatgtgat tttattatct ttttatgctg
     1021 gcgactatgt tgtggcaaat tttaatgctg ctgataaatt aagaatggct gctcaagggt
     1081 tacttattcc aataggacag gctgttttcc cacgattatc taaactagag ggctatgaat
     1141 atagttctaa acttaaaatt tatgcaataa ggtatgctat ttttggtgtt tgcattagtg
     1201 cgggacttgt atttttaggt cccatgttaa ctactattta tttaggcaaa gaatattcgt
     1261 tgtcaggaga atatcttcaa agtatgtttt tactacctgc cactatttca atatcgacta
     1321 tactgagtca atggatgttg atacctcaag gcaaagaaaa aatattaagc agaatctata
     1381 ttctaggcgc cattgtccat ttattatatg catttccttt agtttactat tatggggctt
     1441 ggggcatggt aatatcaatt ttatttactg aagtcttaat tgtattattt atgcttaagg
     1501 ctgtgaaatg acttacttta ctggttttat tttaatattg tttgctatta taattaaaag
     1561 attaactcca agtcaaagca agaaaaatat tgtcttaata gctaatgcgt tttggggaat
     1621 attgttggta ggttatactt tcaatgaaca atatttcgta ccattaagtg caacaacctt
     1681 gttttttata cttgcattct tatttttctt tagtatgact tatattttaa ttgctaggag
     1741 tggaagggtt gttttttctt tcggtactgg ttttatagaa agcaaatata tttactggtt
     1801 tgctgggatg attaatatta ttagtatctg ctttggcatt atccttttat ataataatca
     1861 tttttcttta aaagtaatga gagaaggaat tttagatggt tctattagtg ggtttggatt
     1921 ggggataagt ttgccacttt ccttctgctg tatgtattta gcaagacatg agaataaaaa
     1981 aaattatttc tattgtttta cactactttc attcttgctt gcggtgttat caacttcaaa
     2041 gatcttctta atattattcc ttgtatatat tgttggaata aatagttatg taagcaaaaa
     2101 gaaattgctt atttatggag tgtttgtatt tggactgttc gctttatcaa gtattatctt
     2161 gggtaagttc tcttcagacc ctgaaggcaa gattatttca gcaatatttg atacgttaag
     2221 ggtttatctt ttctcgggat tggcagcctt taatctttat gttgaaaaga atgccacgct
     2281 ccccgaaaat ttacttttgt atccatttaa ggaggtttgg gggacgacaa aagatattcc
     2341 caaaactgat attttgcctt ggatcaacat tggtgtatgg gacacgaatg tatatacagc
     2401 ttttgcacca tggtatcagt cattgggatt atatgcagct ataattattg gtattctctt
     2461 agggttttat tacgggatat ggtttagctt tcgtcaaaat ttagctgtgg gtttttatca
     2521 aacatttttg tgttttcctc ttttaatgtt gtttttccag gagcattatt tgttgtcatg
     2581 gaaaatgcat tttatttatt ttttatgtgc aattttatta gcgatgagaa aagcattaga
     2641 gtatgaataa atattgtatc ttagtactat ttaatccaga tataagtgtt tttattgata
     2701 atgtcaaaaa gattttatct ttggatgtaa gtttatttgt atatgacaat tcagcaaata
     2761 aacatgcatt ccttgctcta tcctcacaag agcaaacaaa gataaattac ttttcgatat
     2821 gtgaaaatat cggattgtcg aaagcttata atgagacact aaggcatatt cttgaattta
     2881 ataagaatgt gaaaaataaa agcattaatg atagtgtgct ttttctcgac caagactctg
     2941 aagttgattt aaattccatc aatattttgt ttgaaactat atcagcagca gagtctaatg
     3001 tgatgatagt cgcggggaat cccataagga gagatggact accgtatata gattaccccc
     3061 acactgtaaa caatgtaaaa tttgtaatta gtagttatgc tgtgtatcgc ttagacgcat
     3121 ttagaaacat cggcttgttt caagaagatt tttttataga tcatatcgat agtgattttt
     3181 gttcaaggct gataaaaagc aattaccaaa ttctccttag aaaagatgcc tttttttatc
     3241 aaccaatagg aataaaacca ttcaatctct gtggtagata tttattccct atcccatcac
     3301 aacaccgaac atattttcaa attagaaatg cttttttaag ttacaggcgc aatggtgtta
     3361 catttaattt tttatttagg gaaattgtaa atagattgat tatgagtata ttctcaggcc
     3421 ttaacgagaa agacttattg aaacgattgc atttatattt aaaaggaata aaagatggtc
     3481 ttaaaatgta attcttggct agaagtgggg gcgttgtgat taaaaaaaaa gtggcggcga
     3541 taattataac atataatcca gatctaacaa ttctgcgaga aagttatacg agtctatata
     3601 agcaagtcga taaaataatt cttattgata acaactctac aaactatcaa gaacttaaga
     3661 agttattcga aaaaaaagaa aaaataaaaa tagtgccctt gagtgataat ataggactag
     3721 cagcagctca aaatttaggt ttgaacttag ctattaaaaa taactatact tatgctattt
     3781 tattcgatca ggatagcgtc ttacaagaca atggaattaa cagtttcttt tttgaatttg
     3841 agaaattagt tagtgaagaa aaattaaata tagttgccat tgggccaagt ttttttgacg
     3901 aaaagacagg aagacgcttt cggcctacaa aatttatcgg tcccttttta tatccctttc
     3961 gtaaaataac cacaaaaaat cctctaacag aagttgactt cttgattgct tctggttgtt
     4021 tcataaaatt ggagtgtatt aaatcagccg gaatgatgac tgaatcgtta ttcatcgatt
     4081 atattgatgt tgaatggtca tatcgtatgc gttcgtatgg ctataagcta tatattcata
     4141 atgatattca catgagtcat ttagtgggag aatctcgagt taatttagga ttgaaaacta
     4201 tttctttaca tgggccgcta agacgatatt acttatttag gaattatatt tcaattttaa
     4261 aagtgagata tataccgtta ggatataaaa tacgtgaggg tttttttaat atcggaagat
     4321 ttttggtaag tatgattata actaaaaata gaaaaacttt aattttatac actataaaag
     4381 caattaagga cggaataaat aatgaaatgg ggaaatataa aggctaacaa catattatga
     4441 aaaaaataat acataaccaa gtgttgccga aaatgtctgg gattcagcaa atctcttttg
     4501 atattttgtc aggtcttaaa gacaaggatg tacaaaaatt tatattatgc ggtttaccag
     4561 atggcagttc agataatgaa tttaaaaaga aatttactga tataggtgtt agggttatta
     4621 cgatacctac attaaaacga aatatcgggt ggcatgactt tcgatgtttc attgatttat
     4681 acaatttttt taaaaaagaa aaatttgata tagttcatac aaactcaact aagccaggaa
     4741 taatagctag aatagccgct agattagccg ggacaaaact cattattcac acagtacatg
     4801 gaatcgcatt tcatagaaaa gaaaatactg taaggaaaat tttgcgactc tttttggtag
     4861 cattaatgtg acagtgaacg agaattattt aaaatattat ccattcgtta aaagtcatat
     4921 tatatataat ggagttgatt tcaacgttct ttgctgcaat aaaaaggatt atgatttttt
     4981 acatattgca tttatggcta gacttgataa acaaaaaaac cattggagtt tataagagcc
     5041 gttaatatta ttaagaaaaa attaccaaat gagcgtttga aatttacatt agctggctgt
     5101 ggtgagttag aaaatgaatg taaaaaatta atagaatatt ttcatcttac agatgttatt
     5161 gatatgcctg gatggatagt agataaaaac acgttttata actctgtcga tattatttgc
     5221 cagccatcca attgggtggc ttttggctta gtatttgttg aggccgcctt ttttgaaatt
     5281 ccatctgttt caaggaatat cgaagggatt cctgaggtta ttttagataa tgaaactggg
     5341 cttttgtatg agggcggaga agccgagttg agtgaaaagc tgatatc
//