From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Chlamydia pneumoniae DnaK protein homolog gene, complete cds.
Date: 29 Jul 92 10:01:29 GMT
Distribution: bionet
Checksum: 42792     6

LOCUS       CHTDNAKHPA   2373 bp ds-DNA             BCT       29-JUL-1992
DEFINITION  Chlamydia pneumoniae DnaK protein homolog gene, complete cds.
ACCESSION   M69227 X60083
KEYWORDS    DnaK protein homologue; HSP70; heat shock protein 70.
SOURCE      Chlamydia pneumoniae (strain TWAR) DNA.
  ORGANISM  Chlamydia pneumoniae
            Prokaryotae; Gracilicutes; Scotobacteria;
            Rickettsias and Chlamydias; Chlamydiales; Chlamydiaceae.
REFERENCE   1  (bases 1 to 2373)
  AUTHORS   Kornak,J.M., Kuo,C.C. and Campbell,L.A.
  TITLE     Sequence analysis of the gene encoding the Chlamydia pneumoniae
            DnaK protein homolog
  JOURNAL   Infect. Immun. 59, 721-725 (1991)
  STANDARD  simple staff_review
FEATURES             Location/Qualifiers
     -35_signal      50..55
                     /note="putative"
     -10_signal      72..77
                     /note="putative"
     RBS             168..174
                     /note="putative"
     CDS             182..2164
                     /note="DnaK protein homolog; 71,550 Da"
                     /product="DnaK protein homologe"
                     /note="putative"
                     /codon_start=1
                     /translation="MSEHKKSSKIIGIDLGTTNSCVSVMEGGQAKVITSSEGTRTTPS
                     IVAFKGNEKLVGIPAKRQAVTNPEKTLGSTKRFIGRKYSEVASEIQTVPYTVTSGSKG
                     DAVFEVDGKQYTPEEIGAQILMKMKETAEAYLGETVTEAVITVPAYFNDSQRASTKDA
                     GRIAGLDVKRIIPEPTAAALAYGIDKVGDKKIAVFDLGGGTFDISILEIGDGVFEVLS
                     TNGDTLLGGDDFDEVIIKWMIEEFKKQEGIDLSKDNMALQRLKDAAEKAKIELSGVSS
                     TEINQPFITMDAQGPKHLALTLTRAQFEKLAASLIERTKSPCIKALSDAKLSAKDIDD
                     VLLVGGMSRMPAVQETVKELFGKEPNKGVNPDEVVAIGAAIQGGVLGGEVKDVLLLDV
                     IPLSLGIETLGGVMTTLVERNTTIPTQKKQIFSTAADNQPAVTIVVLQGERPMAKDNK
                     EIGRFDLTDIPPAPRGHPQIEVSFDIDANGIFHVSAKDVASGKEQKIRIEASSGLQED
                     EIQRMVRDAEINKEEDKKRREASDAKNEADSMIFRAEKAIKDYKEQIPETLVKEIEER
                     IENVRNALKDDAPIEKIKEVTEDLSKHMQKIGESMQSQSASAAASSAANAKGGPNINT
                     EDLKKHSFSTKPPSNNGSSEDHIEEADVEIIDNDDK"
     stem_loop       2192..2217
                     /note="putative 11-base dyad terminator"
                     /note="putative"
     stem_loop       2217..2271
                     /note="second stem loop structure"
                     /note="putative"
BASE COUNT      835 a    499 c    487 g    552 t
ORIGIN
        1 cgaagcgggt gcaaacagaa gagacttctg aagttcctga ggggacgatt ttagaagagt
       61 ttgcaaaggg atataaaata ggagaacgcc cgattcgggt agctaaagtt aaagtcgcta
      121 aagctcctac tcccaaagaa aataaagaat agaaataacc cctagagatt aggtaccaaa
      181 catgagtgaa cacaaaaaat caagcaaaat tataggtata gacttaggca caacaaactc
      241 ctgcgtatct gttatggaag gaggacaagc taaagtaatt acatcatccg aaggaacaag
      301 aaccacgcca tcgatcgttg ccttcaaagg taatgagaaa ttagtgggga ttccagcaaa
      361 acgtcaagca gtgacaaatc cagaaaaaac tctcggctct acaaaacgct ttattggccg
      421 taagtactct gaagtagctt cggaaatcca aaccgttcct tatacagtca cctccggatc
      481 taaaggtgat gccgttttcg aagttgatgg caaacaatac actccagaag aaattggcgc
      541 acaaatctta atgaaaatga aagagacagc agaagcttat ctaggcgaaa ctgtcacaga
      601 agcagtgatc accgtccccg catacttcaa tgattctcaa cgagcatcca caaaagatgc
      661 tggacgcatt gcaggtctag atgtaaaacg tatcattcca gaacctaccg cagcagctct
      721 tgcctacgga atcgataaag tcggtgataa aaaaatcgct gtcttcgacc ttggtggagg
      781 aacttttgat atctccatcc tagaaatcgg tgatggcgtc ttcgaagttc tatctacaaa
      841 tggagatact ctcctcggtg gagacgactt tgatgaagtc attatcaaat ggatgatcga
      901 agaattcaaa aaacaagaag gcattgatct tagcaaagat aatatggcct tacaaagact
      961 taaagatgct gctgagaaag caaaaataga actttcagga gtctcttcca cagaaatcaa
     1021 tcagccattc atcacaatgg atgcacaagg acctaaacac cttgcattga cactcacacg
     1081 tgcgcaattc gagaaactcg cagcctctct aatcgaaaga acaaaatctc catgcatcaa
     1141 agcactcagt gacgcaaaac tttccgctaa ggatatcgat gatgttctct tagttggagg
     1201 tatgtcaaga atgcccgcag tgcaagaaac tgtaaaagaa ctcttcggca aagagcctaa
     1261 taaaggagtc aaccccgacg aagttgttgc tattggagcc gcaattcaag gtggtgttct
     1321 tggcggagaa gttaaggatg ttctacttct agacgttatc cccctatctc tgggtatcga
     1381 aactctagga ggcgtcatga cgactctggt agagagaaat actacaatcc ctacacagaa
     1441 aaaacaaatc ttctccacag ctgctgataa ccagcctgcg gttaccatcg tagttctcca
     1501 aggagagcgt cccatggcca aagataacaa ggaaatcgga agattcgatc ttacagatat
     1561 ccctccggct cctcgaggcc atcctcaaat cgaagtctcc ttcgatatcg atgcaaacgg
     1621 aattttccat gtctcagcta aagatgttgc cagcggtaaa gaacagaaaa ttcgtatcga
     1681 agcaagctca ggacttcaag aagatgaaat ccaaagaatg gttcgagatg ccgaaattaa
     1741 taaggaagaa gataaaaaac gtcgtgaagc ttcagatgct aaaaatgaag ccgatagcat
     1801 gatcttcaga gccgaaaaag ctattaaaga ttataaggag caaattcctg aaactttagt
     1861 taaagaaatc gaagagcgaa tcgaaaacgt gcgcaacgca ctcaaagatg acgctcctat
     1921 tgaaaaaatt aaagaggtta ctgaagacct aagcaagcat atgcaaaaaa ttggagagtc
     1981 tatgcaatcg cagtctgcat cagcagcagc atcatcggca gccaatgcta aaggtggacc
     2041 taacatcaat acagaagatt tgaaaaaaca tagtttcagt acgaagcctc cttcaaataa
     2101 cggttcttca gaagaccata tcgaagaagc tgatgtagaa attattgata acgacgataa
     2161 gtaatcaaaa ttttcaattt aagtttctct attcccatcc tcataagagg atgggaaact
     2221 tccttataaa cagaaaacag ttccattctc ttattctctg atcaaggagt tgcaataaca
     2281 gagcttcttt agtgcaattg gctttgaatt tgagactgct cctttcataa tcacaaaacc
     2341 cacttaaaag ggaaaatttt gttgagccac tca
//