From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Pseudomonas oleovorans alkBFGH genes from the alkBAC operon.
Date: 22 Dec 92 22:56:47 GMT
Distribution: bionet
Checksum: 00766 10

LOCUS       PSEALKBFG    4584 bp ds-DNA             BCT       30-NOV-1992
DEFINITION  Pseudomonas oleovorans alkBFGH genes from the alkBAC operon.
ACCESSION   J04618 J04619
KEYWORDS    aldehyde dehydrogenase; alkB gene; alkF gene; alkG gene; alkH gene;
            alkane hydroxylase; alkane monooxygenase; rubredoxin 1;
            rubredoxin 2.
SOURCE      Pseudomonas oleovorans (strain P.deo TF4-1L) DNA, isolate GPo1,
            clones pGEc2g, pGEc48, and pGEc150.
  ORGANISM  Pseudomonas oleovorans
            Prokaryotae; Gracilicutes; Scotobacteria; Pseudomonadaceae.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Kok,M., Oldenhuis,R., von der Linden,M.P., Raatjes,P., Kingma,J.,
            van Lelyveld,P.H. and Witholt,B.
  TITLE     The Pseudomonas oleovorans alkane hydroxylase gene: Sequence and
            expression
  JOURNAL   J. Biol. Chem. 264, 5435-5441 (1989)
  STANDARD  full automatic
REFERENCE   2  (bases 1997 to 4584)
  AUTHORS   Kok,M., Oldenhuis,R., von der Linden,M.P., Meulenberg,C.H.,
            Kingma,J. and Witholt,B.
  TITLE     The Pseudomonas oleovorans alkBAC operon encodes two structurally
            related rubredoxins and an aldehyde dehydrogenase
  JOURNAL   J. Biol. Chem. 264, 5442-5451 (1989)
  STANDARD  full automatic
COMMENT     Draft entry and computer-readable copy of sequence [1],[2] kindly
            provided by M.Kok, 30-JAN-1989.
FEATURES             Location/Qualifiers
     prim_transcript 526..>4584
                     /note="alkBFGHJKL mRNA"
     CDS             622..1827
                     /note="alkanehydroxylase;  (alkB)"
                     /codon_start=1
                     /translation="MLEKHRVLDSAPEYVDKKKYLWILSTLWPATPMIGIWLANETGW
                     GIFYGLVLLVWYGALPLLDAMFGEDFNNPPEEVVPKLEKERYYRVLTYLTVPMHYAAL
                     IVSAWWVGTQPMSWLEIGALALSLGIVNGLALNTGHELGHKKETFDRWMAKIVLAVVG
                     YGHFFIEHNKGHHRDVATPMDPATSRMGESIYKFSIREIPGAFIRAWGLEEQRLSRRG
                     QSVWSFDNEILQPMIITVILYAVLLALFGPKMLVFLPIQMAFGWWQLTSANYIEHYGL
                     LRQKMEDGRYEHQKPHHSWNSNHIVSNLVLFHLQRHSDHHAHPTRSYQSLRDFPGLPA
                     LPTGYPGAFLMAMIPQWFRSVMDPKVVDWAGGDLNKIQIDDSMRETYLKKFGTSSAGH
                     SSSTSAVAS"
     CDS             2044..2442
                     /note="rubredoxin 1;  (alkF)"
                     /codon_start=1
                     /translation="MSRYQCPDCQYIYDENKGEPHEGFHPNTSWNDIPKDWACPDCAV
                     RDKVDFIFLADSPSKETQLGVNSQLANSESGISDATPTGMAVLAAELVIPLNQENKNE
                     GCAAKTEVLDQASTPQVVRKSSTRKKMRNK"
     CDS             2490..3011
                     /note="rubredoxin 2;  (alkG)"
                     /codon_start=1
                     /translation="MASYKCPDCNYVYDESAGNVHEGFSPGTPWHLIPEDWCCPDCAV
                     RDKLDFMLIESGVGEKGVTSTHTSPNLSEVSGTSLTAEAVVAPTSLEKLPSADVKGQD
                     LYKTQPPRSDAQGGKAYLKWICITCGHIYDEALGDEAEGFTPGTRFEDIPDDWCCPDC
                     GATKEDYVLYEEK"
     CDS             3058..4509
                     /note="aldehyde dehydrogenase;  (alkH)"
                     /codon_start=1
                     /translation="MTIPISLAKLNSSADTHSALEVFNLQKVASSARRGKFGIAERIA
                     ALNLLKETIQRREPEIIAALAADFRKPASEVKLTEIFPVLQEINHAKRNLKDWMKPRR
                     VRAALSVAGTRAGLRYEPKGVCLIIAPWNYPFNLSFGPLVSALAAGNSVVIKPSELTP
                     HTATLIGSIVREAFSVDLVAVVEGDAAVSQELLALPFDHIFFTGSPRVGKLVMEAASK
                     TLASVTLELGGKSPTIIGPTANLPKAARNIVWGKFSNNGQTCIAPDHVFVHRCIAQKF
                     NEILVKEIVRVYGKDFAAQRRSADYCRIVNDQHFNRINKLLTDAKAKGAKILQGGQVD
                     ATERLVVPTVLSNVTAAMDINHEEIFGPLLPIIEYDDIDSVIKRVNDGDKPLALYVFS
                     EDKQFVNNIVARTSSGSVGVNLSVVHFLHPNLPFGGVNNSGIGSAHGVYGFRAFSHEK
                     PVLIDKFSITHWLFPPYTKKVKQLIGITVKYLS"
BASE COUNT     1189 a    978 c   1184 g   1233 t
ORIGIN      
        1 ctgcagcgcc tgctccagcg tggcgtgata agggctgagt ttgttgaaga tcgcgcggcg
       61 ttggtaaacc ggctgcttcg cctcgggtgc tctgacccac ttgcgaatcg tgttgcgcgc
      121 caacccggtg cgcttggcta tctcatgcag cgacagcttg tcgcggaaat acatccggcg
      181 aattttgccc atcatttcca tactgatcac cctgtgttct cctgctcaaa aattgagcag
      241 aagcagttga acacctgggt cagttttcag tcggcagaac agcctctact gggtcagttt
      301 tcggtcagcg gcaacacaag gggacgccaa tgttacccgg aagcccgggt tttggaggtt
      361 tccaatcgtg ccttggcgca gcgacagccc tcggtccccc agatagccat tgatcaccgc
      421 cagcatgctt gcggccagct cgtgtttttc cagcagacga cggagcaaaa actacccgta
      481 ggtgtagttg gcgcaagcgt ccgattagct caggtttaag atgtcgagag tgagagtggg
      541 cggcttaact ttctcagtta ggcataaaat tacgtcttaa atctcgtagc gactaattta
      601 ataaaaattg gagaacacca aatgcttgag aaacacagag ttctggattc cgctccagag
      661 tacgtagata aaaagaaata tctctggata ctatcaactt tgtggccggc tactccgatg
      721 atcggaatct ggcttgcaaa tgaaactggt tgggggattt tttatgggct ggtattgctc
      781 gtatggtacg gcgcacttcc attgcttgat gcgatgtttg gtgaggactt taataatccg
      841 cctgaagaag tggtgccgaa actagagaag gagcggtact atcgagtttt gacatatcta
      901 acagttccta tgcattacgc tgcattaatt gtgtcagcat ggtgggtcgg aactcagcca
      961 atgtcttggc ttgaaattgg tgcgcttgcc ttgtcactgg gtatcgtgaa cggactagcg
     1021 ctcaatacag gacacgaact cggtcacaag aaggagactt ttgatcgttg gatggccaaa
     1081 attgtgttgg ctgtcgtagg gtacggtcac ttctttattg agcataataa gggtcatcac
     1141 cgtgatgtcg ctacaccgat ggatcctgca acatcccgga tgggagaaag catttataag
     1201 ttttcaatcc gtgagatccc aggagcattt attcgtgctt gggggcttga ggaacaacgc
     1261 ctttcgcgcc gtggccaaag cgtttggagt ttcgataatg aaatcctcca accaatgatc
     1321 atcacagtta ttctttacgc cgttctcctt gccttgtttg gacctaagat gctggtgttc
     1381 ctgccgattc aaatggcttt cggttggtgg cagctgacca gtgcgaacta tattgaacat
     1441 tacggcttgc tccgtcaaaa aatggaggac ggtcgatatg agcatcaaaa gccgcaccat
     1501 tcttggaata gtaatcacat cgtctctaat ctagtgctgt tccaccttca gcggcactcg
     1561 gatcaccacg cgcatccaac acgttcttat cagtcacttc gggattttcc cggcctgccg
     1621 gctcttccga cgggttaccc tggtgcattt ttgatggcga tgattcctca gtggtttaga
     1681 tcagttatgg atcccaaggt agtagattgg gctggtggtg accttaataa gatccaaatt
     1741 gatgattcga tgcgagaaac ctatttgaaa aaatttggca ctagtagtgc tggtcatagt
     1801 tcgagtacct ctgcggtagc atcgtagtta tgtgagcacg cagagcccgg cggtcgatat
     1861 ttacaataag tgcttcaatt ttatgtgcgg cgttgaaagc tctcacaaag agtgcacttc
     1921 gctaaagtgc tgagggttga ttgcctctct gtaattgctt tgaaggcgac ctgctccgat
     1981 agttacactc tgatgaagtt gtcggagcag cgactaacgc tgagttaata ggagagtggg
     2041 agaatgtcaa ggtaccagtg tccagattgt cagtatatct atgatgaaaa taagggggag
     2101 ccgcacgaag gtttccaccc gaacaccagc tggaatgata tccccaaaga ttgggcatgc
     2161 ccggactgcg cagttcgaga caaggtggac tttatctttc tcgcggattc tccctcgaaa
     2221 gaaacacagc taggggtgaa tagtcagctt gccaactcgg aaagtggtat ttcagatgct
     2281 actccaactg gaatggcagt tttggccgca gaattagtga tcccacttaa tcaagaaaat
     2341 aaaaatgagg gctgtgcggc taagactgaa gttcttgatc aggcgagcac cccacaggtt
     2401 gtaagaaaat cttccacaag gaagaagatg agaaataaat aacgcaaatt tgccgcaacg
     2461 caaaataaca atttgacatg gtgatgagta tggctagcta taaatgcccg gattgtaatt
     2521 atgtttatga tgagagtgcg ggtaatgtgc atgaggggtt ttctccaggt acgccttggc
     2581 accttattcc tgaggattgg tgctgccccg attgcgccgt tcgagacaag cttgacttca
     2641 tgttaattga gagcggcgta ggtgaaaagg gcgtcacctc aacccatact tcgccaaatt
     2701 tatccgaggt tagtggcaca agtttaactg ctgaagcagt ggttgcgccg acaagcttag
     2761 agaaattgcc tagtgccgac gttaaaggcc aagatctata taaaactcaa cctccaaggt
     2821 ctgatgccca aggcgggaaa gcatacttga agtggatatg tattacttgt ggccatatat
     2881 atgatgaggc gttgggcgat gaggccgagg gttttactcc aggtactcgc tttgaggata
     2941 ttcctgatga ctggtgctgt ccggattgcg gggctacgaa agaagactat gtgctctacg
     3001 aggaaaagtg aagattaaaa cttcaagtca ttctaggtaa ttcaggacaa aataaaaatg
     3061 accataccaa ttagcctagc caagttaaac tctagtgccg atacccattc agcgcttgaa
     3121 gtatttaatt tgcagaaagt tgcaagtagt gcgcgtcgtg gtaaatttgg catagcagag
     3181 cgcatcgctg ctcttaattt acttaaggaa actattcagc gtcgtgagcc tgaaattatt
     3241 gctgcacttg cagcggactt tcgcaagccg gcaagcgagg tgaagctaac agaaatcttt
     3301 ccggtattgc aagaaattaa tcatgccaaa cggaacctta aagattggat gaagccacgg
     3361 cgagtgaggg cggcacttag tgtagcgggc acgcgggcag gacttcgtta cgagcctaag
     3421 ggtgtctgtt tgataattgc gccgtggaac tatccattca accttagttt cggtcctctt
     3481 gtatctgcgt tagcggcagg aaatagcgtt gttataaagc cgtctgaatt gacaccacac
     3541 actgcaacac tgatcggatc tatagtcagg gaggcattct ctgtcgacct agtcgctgtg
     3601 gtggagggtg atgccgcagt ttcccaggag ctgttggctc tgccatttga ccatattttt
     3661 tttactggta gtcctagggt cggcaagtta gtgatggaag cggcgtcaaa aacactcgct
     3721 tcggttactt tggagttagg cggaaaatct ccaaccatta ttggaccaac agcaaatttg
     3781 ccgaaagctg cgcgcaacat agtgtgggga aagttttcaa acaacggcca gacgtgcata
     3841 gcgcctgatc acgtatttgt tcatcggtgt atagcccaga aattcaatga aattcttgtg
     3901 aaagagattg tgcgagttta tgggaaggat tttgctgcgc agcgtagatc ggcagactat
     3961 tgcaggatcg tcaatgatca acatttcaat cgaattaata aactcctgac tgacgcgaaa
     4021 gctaaaggtg caaaaattct gcaagggggt caagttgacg cgactgagag gcttgtggtg
     4081 ccaacggttt tatctaacgt cactgctgct atggatatta accatgagga aatattcggg
     4141 ccgctacttc ctataattga atacgatgat atagattctg taattaagcg tgtgaatgac
     4201 ggtgacaagc ccctggcgct gtatgtcttt tctgaagata aacaatttgt aaataacatc
     4261 gtggctcgta caagctctgg ttcggtcgga gttaatctga gtgtcgtgca ctttttgcac
     4321 cctaatctcc catttggcgg tgtcaataat agtggtatcg gcagtgctca tggagtttac
     4381 gggttcaggg cgttttctca cgaaaaacca gttcttatag ataagttctc aatcacgcat
     4441 tggttgtttc cgccttatac caagaaggtg aagcagttga ttggtatcac agttaagtat
     4501 ttgagctgag taattttttc gataaatcat tacctcgaga agataaaatg tacgactata
     4561 taatcgttgg tgctggatct gcag
//