From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: N.tabacum mRNA for pre-pro-cysteine proteinase
Date: 28 Jul 92 10:08:03 GMT
Distribution: bionet
Checksum: 18950     5

LOCUS       TOBCYP8      1330 bp ss-RNA             PLN       28-JUL-1992
DEFINITION  N.tabacum mRNA for pre-pro-cysteine proteinase
ACCESSION   Z13964
KEYWORDS    cysteine proteinase.
SOURCE      Nicotiana tabacum RNA.
  ORGANISM  Nicotiana tabacum
            Eukaryota; Plantae; Embryobionta; Magnoliophyta; Magnoliopsida;
            Asteridae; Solanales; Solanaceae.
REFERENCE   1  (bases 1 to 1330)
  AUTHORS   Linthorst,H.J.
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
REFERENCE   2  (bases 1 to 1304)
  AUTHORS   Linthorst,H.J., Van der Does,C., Brederode,F.T. and Bol,J.F.
  TITLE     Circadian expression and induction by wounding of tobacco genes for
            cysteine proteinase
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
COMMENT     *source: strain=cv. Samsun NN; *source: stage=Acutely infected;
            *source: tissue=Leaf; *source: clone_library=Lambda ZAP
            TMV-tobacco; *source: clone=CYP-8;
            From EMBL    entry NTCYP8;  dated 30-JUN-1992.
FEATURES             Location/Qualifiers
     CDS             22..1119
                     /function="Putative precursor of cysteineproteinase"
                     /product="tobacco pre-pro-cysteineproteinase"
                     /codon_start=1
                     /translation="MDRLFLLSLPRFALFSSAIAFPDEDPLIRQVVSETETDDSHLLN
                     AEHHFSLFKSKFGKIYASEEEHDHRFKVFKANLRRARLNQLLDPSAEHGITKFSDLTP
                     SEFRRTYLGLHKPKPKVNAEKAPILPTSDLPADYDWRDHGAVTGVKNQGSCGSCWSFS
                     TTGAVEGAHFLATGELVSLSEQQLVDCDHECDSEQQDSCDAGCGGGLMTTAFEYTLKA
                     GGLQLEKDYPYTGKDGKCHFDKSKIAAAVTNFSVIGLDEDQIAANLVKHGPLAVGINA
                     AWMQTYVGGVSCPLICFKRQDHGVLLVGYGSHGFAPIRLKEKAYWIIKNSWGENWGEH
                     GYYKICRGHNICGVDAMVSTVTAAHTTNPNL"
     sig_peptide     22..76
     mat_peptide     415..1116
                     /function="protein degradation"
                     /note="Matureprotein putatively processed from
                     pre-pro-protein,based on similarity to other cysteine
                     proteinases"
                     /product="tobacco cysteine proteinase"
     misc_feature    487..489
                     /product="encodes active Cys residue"
     misc_feature    913..915
                     /product="encodes active His residue"
     polyA_site      1304
                     /evidence=EXPERIMENTAL
                     /citation=[1]
                     /citation=[2]
BASE COUNT      336 a    300 c    311 g    383 t
ORIGIN
        1 ctcattttat cttttctcca aatggatcgt ctatttctct tatctcttcc tcgtttcgca
       61 cttttctcgt cggcgatagc gttccccgac gaggatccgt tgatccgaca agtcgtgtcc
      121 gaaaccgaaa ccgacgacag tcatctgtta aacgccgaac atcacttttc actttttaag
      181 tcaaagttcg gaaagatcta cgcttcggag gaggaacatg accataggtt caaagtattc
      241 aaggcgaacc tccgccgtgc ccggctcaat cagctccttg acccttctgc cgaacacggt
      301 attacgaagt tctccgatct tactccgtcg gagtttcgcc ggacttacct cggacttcac
      361 aaaccaaaac ctaaagttaa cgctgaaaaa gctccaatcc tcccgacttc cgatcttcca
      421 gctgattatg actggcgtga ccacggcgcc gtcaccggcg taaaaaatca gggttcatgc
      481 ggttcatgct ggtcgttcag tacaacagga gcggtggaag gagctcactt tttagcgacg
      541 ggagagttgg tgagccttag tgaacagcag cttgtggact gtgaccatga gtgtgactcg
      601 gagcaacaag attcttgtga tgcaggttgc ggtggtggtc ttatgacgac tgcctttgag
      661 tacaccctga aagctggagg tcttcaactg gagaaagact atccttacac tggcaaggat
      721 ggcaaatgtc actttgacaa gagcaaaatt gctgcagctg tgactaactt cagtgttatt
      781 ggtcttgatg aagaccaaat tgctgccaac ctggttaaac atggtcctct tgcagtgggg
      841 atcaacgctg cttggatgca gacatatgtt ggaggagttt catgcccatt aatttgcttc
      901 aagcgtcagg atcacggagt gctcttagta ggttatggtt ctcatggctt tgctcctatc
      961 agactcaagg aaaaggctta ctggatcatc aagaattcat ggggagagaa ctggggagag
     1021 catggatatt acaagatttg caggggtcac aatatctgtg gagttgatgc catggtctct
     1081 actgtgactg ctgcccatac tactaatcct aacctgtgag atgaacttat cccaggaatg
     1141 ttgtctgtga tcaagggttt tactacatta tcctaatttg tgtaaatatg tcgttcagta
     1201 aatgtactgt gaactcaagt cttagattcc atcagcactt tattatcgtg cttcaaacca
     1261 cgtattggga tttgtatttc tgtagtttat acctgcaaaa atgaagttcg aattttaaag
     1321 ctgtttgtat
//