From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: D.melanogaster (clones T-beta-1E, T-beta-1J) guanine
Date: 27 Jan 93 05:46:58 GMT
Distribution: bionet
Checksum: 61531 10

LOCUS       DROGGBCS     4590 bp ds-DNA             INV       26-JAN-1993
DEFINITION  D.melanogaster (clones T-beta-1E, T-beta-1J) guanine
            nucleotide-binding regulatory protein (Gbb) gene, complete cds.
ACCESSION   M22567 J04083 L05555 L06051 L06052 L06053 M22566
KEYWORDS    guanine nucleotide-binding regulatory protein;
            highly expressed gene; signal transduction.
SOURCE      Drosophila melanogaster (strain Canton S) (library: Charon 4;
            Maniatis (1978)) adult DNA.
  ORGANISM  Drosophila melanogaster
            Eukaryota; Animalia; Metazoa; Arthropoda; Uniramia; Insecta;
            Pterygota; Neoptera; Holometabola; Diptera; Brachycera;
            Cyclorrhapha; Schizophora; Drosophiloidea; Drosophilidae.
REFERENCE   1  (bases 1 to 4590)
  AUTHORS   Yarfitz,S., Provost,N.M. and Hurley,J.B.
  TITLE     Cloning of a Drosophila melanogaster guanine nucleotide regulatory
            protein beta-subunit gene and characterization of its expression
            during development
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 85, 7134-7138 (1988)
  STANDARD  full automatic
REFERENCE   2  (sites)
  AUTHORS   Yarfitz,S., Niemi,G.A., McConnell,J.L., Fitch,C.L. and Hurley,J.B.
  TITLE     A Gbeta Protein in the Drosophila Compound Eye is Different from
            That in the Brain
  JOURNAL   Neuron 7, 429-438 (1991)
  STANDARD  full automatic
COMMENT     Draft entry and computer-readable sequence for [1] kindly provided
            by S.Yarfitz, 13-JAN-1989.
FEATURES             Location/Qualifiers
     exon            one-of(1265..1376,1265..1448)
                     /number=1
                     /gene="Gbb"
     mRNA            join(one-of(1265..1376,1265..1448),2543..2663,2737..4075)
                     /gene="Gbb"
                     /citation=[2]
                     /note="this gene is abundantly expressed in brain and
                     thoracic ganglion"
     misc_feature    1..2041
                     /note="clone T-beta-1E"
     misc_feature    2042..4590
                     /note="clone T-beta-1J"
     exon            2543..2663
                     /number=2
                     /gene="Gbb"
     variation       replace(2574..2576,"aa")
                     /gene="Gbb"
                     /citation=[1]
                     /note="in clone T-beta-C3F"
     exon            2737..4075
                     /number=3
                     /gene="Gbb"
     variation       replace(2832,"g")
                     /gene="Gbb"
                     /citation=[1]
                     /note="in clone T-beta-C6R"
     variation       replace(3572,"t")
                     /gene="Gbb"
                     /citation=[1]
                     /note="in clone T-beta-C6R"
     CDS             2945..3967
                     /gene="Gbb"
                     /citation=[2]
                     /note="this gene is abundantly expressed in brain and
                     thoracic ganglion"
                     /product="guanine nucleotide-binding regulatory protein"
                     /codon_start=1
                     /translation="MNELDSLRQEAESLKNAIRDARKAACDTSLLQAATSLEPIGRIQ
                     MRTRRTLRGHLAKIYAMHWGNDSRNLVSASQDGKLIVWDSHTTNKVHAIPLRSSWVMT
                     CAYAPSGSYVACGGLDNMCSIYNLKTREGNVRVSRELPGHGGYLSCCRFLDDNQIVTS
                     SGDMSCGLWDIETGLQVTSFLGHTGDVMALSLAPQCKTFVSGACDASAKLWDIREGVC
                     KQTFPGHESDINAVTFFPNGQAFATGSDDATCRLFDIRADQELAMYSHDNIICGITSV
                     AFSKSGRLLLAGYDDFNCNVWDTMKAERSGILAGHDNRVSCLGVTENGMAVATGSWDS
                     FLRVWN"
BASE COUNT     1461 a    977 c    940 g   1211 t      1 others
ORIGIN      1 bp upstream of EcoRI site.
        1 ctgcacagat taagctgttt ccaccagtcc gacctgacgg attcatcgaa gagcctttca
       61 tttaaattga ctatatttgg cgttcgcgga aaatcccgca gacatcgggc gctccaataa
      121 cgccggcctc gttccgagaa tggattccga atcacaataa tgcccgggat ggttagttaa
      181 tgtataggat cgccaatctt ttggcgattt aacgcctggc attgtctgta ggctttgtac
      241 attgctgata cttaatcgtt gaacaatatt ctgaggggaa tgtagaaatc ataattcaat
      301 tgcaacggat cgagtactta cattatcact ctttttcttg atcattccct tcgactacac
      361 aactaatcga tcttaacaac ttcgttaact aggactggta ctttgcactt gtactgctta
      421 aaactagctt gaacctgctt caaaaaccat aagtaccata ataaaccatt aaggaataaa
      481 aaccgaatag gcaataggtn tgacctgtcg cggttggaga aatcttaaat gcttttgagc
      541 acgtttacaa atcagacatg gggtggaaat ctaggataga tataaattaa aataaatgca
      601 ataaggacat gccgaaagct cgatgtcttt catgcaaaaa tttagccttt tttcatgagg
      661 tgatcatttg tgattataat cttatcgata taaagttatc gatatatgaa cggattttcg
      721 taatgcaatc atttttaact attttgaaat agttgttttg agccgatttt ttcattgttt
      781 acgtttttgc agtgatcgtt ttcattattt cggtcgaaga tacgtcttaa gggttgcaca
      841 gaaaaaaatc aagtgggaac attacacttc agagcgataa tagatcttta ttttttatat
      901 ttttatgaat taaaattaaa aaaggagttg aaataagaaa tggtatattt atatgacata
      961 tttatatata tatatattgt tgtagttatt tactttcagt gaggggcgtg cttaatacta
     1021 tattcggttc aaaagcaaag gggtaccaca ttgtttatat aaaataaaat atgtttttaa
     1081 aatataacgt cttttgacaa ataaactttg aataaataaa gacaagttgg tatatttgcc
     1141 ggctctctcc gtccgcagtg tgaacagcct ggttgccgaa tatgtatcaa tgctatcgat
     1201 atccgcatac atcgatactg cttcccttgc acccttaatt tgtttctagg tgttggctgc
     1261 caattgtaga gtgcaaatga aaagttacag attggtggct tcgttaacaa aattttcagt
     1321 tatttgtgta gttgaagacg ggaatttgtt ttgcgtataa aaagttaaca ctgcgtgtaa
     1381 gtaacgtatt gtcgtcatca acaaactgtc cagtgaatga aaattgaaag aaaaattact
     1441 gatctatggt aatgtgtgtg tatgtgctaa aaggaaaaaa aaatttttca tactcgcgtg
     1501 aacgacgagt gatgaaaatg cagtctcgat agttttgcgc ctacatatat gtatgactat
     1561 tgcacagatt tggttgtttt ttttttctaa acgattgcca gtcggcatgg aaaattaaat
     1621 gaaatgagct ccatatattt tgtgcctgtg ccagtgcctt ccaacgaaaa atgttcaacg
     1681 tttaaaattg attatttttt ttttatctat ttccctcctg ctctttgtca tgtgtacgct
     1741 tacgcggttg ctgcgcgtgc gaatgggaca ccgattgccg cgactgtttt tgtctcaccg
     1801 cccgctttcg ggaacttctg acctgctttc gctgccgcga tttcaattgc gcacggctcg
     1861 catccactag tcgtgctcct tgctccaatc gaaagggcca cttgcgccgc ggtggtggtg
     1921 caaaacttgc aattttggtg tacaaaaaaa gcaaaaggaa tatatcccat atatcctacc
     1981 catctatcgg aaggaacgga attcgagctc ggtacccggg gatcctctag agtcgaccga
     2041 attcgaaatc cagcacataa aaagcataaa tcacatcaaa aaattcgcat tttctctggt
     2101 ccttagcacc agatcgcttt ttatgcatcc aattcggttt cctctttttt tttagtatac
     2161 catcccatcc gcgagataac attctgtatt aaacaaaacg cccaacatcc taattacaaa
     2221 tcagacggga cggaaaatcg aaaaaactat aggaacctct ctctcccccc ccactaacgt
     2281 agtttatgtg tatttaacaa acgaagaact ttctgcagag gtcgaaatac accaatcgca
     2341 cataccaaac aaaaagtaat aaaaacgaaa taattgaaaa accaaatagt taaattggca
     2401 gagcaaacac aggacgacga ctatgaacta aagtcagaaa ttaatggaca tcgtaccgag
     2461 aatataatcc gtgaatagta ctatacgata aaacagaaaa agaaaaaaag tcaactaaaa
     2521 agcaaaaaac caactcttgc agaacacagt tcatgattta tcctatccat actagaagtg
     2581 cacaaaataa acgccgtatg caagcgtgat catccaaggc tcattatccg actaagctac
     2641 tggccagctt aaaaaaagta agcgtaagta cacccacaat tgaccatata actaggttaa
     2701 tgaacgcctc catttactac ttccccatat gtatagcacc atctaacaag agcgaatcga
     2761 atcgaatcga cttggacgac cgaccgaccg accgcacaaa gaaggatcta tctggaaagc
     2821 cggctagccg attcccgcat ccgattccga atacgaatac gtatccgtat ccgaatccga
     2881 atttgaaacc gaatcctcaa ccaaatcatc ccataaggac gccgctgtct tcaacgatca
     2941 caagatgaat gaactagaca gtctcaggca ggaagccgag tccctaaaga acgccattcg
     3001 ggatgcccgg aaggcggcct gcgacacatc actgttgcaa gcggccacct cgctggaacc
     3061 catcggccgc atacagatgc gcacccgtcg tacattacgc ggccatttgg cgaaaatcta
     3121 cgccatgcat tggggcaacg attcaaggaa tctcgtatca gcctcacagg acggcaaact
     3181 gatcgtttgg gactcgcata ccacgaacaa agtccatgcc attccactgc gatcctcgtg
     3241 ggtgatgacc tgtgcgtacg ccccatccgg tagctatgtg gcctgcggtg gcctcgacaa
     3301 catgtgttca atttacaacc taaagacgcg cgagggcaac gtccgggtgt cccgtgagct
     3361 gcccggccat ggtggctatc tatcgtgctg ccgcttcctg gacgacaatc agatcgtgac
     3421 cagctccggt gatatgtcgt gcggattgtg ggatatcgag acgggactgc aggtaacctc
     3481 gtttttgggc cacaccggcg atgtgatggc cctctcactg gcgccccaat gcaaaacgtt
     3541 cgtatccggc gcctgcgatg cgtccgccaa gctatgggac atccgggagg gtgtctgtaa
     3601 acaaaccttc cccggccacg aatccgatat caatgcggtc acatttttcc cgaatggtca
     3661 ggcattcgcc accggttcgg acgacgcaac ctgtcgattg ttcgatatcc gtgccgatca
     3721 ggagttggcc atgtattcgc acgacaacat catatgcggc atcacatcgg tggcattctc
     3781 gaagagcgga cgtctgttat tagcgggcta cgatgatttc aactgcaatg tatgggacac
     3841 gatgaaggca gaacggtctg gcatactcgc tggccacgac aaccgtgtat cctgtttggg
     3901 tgtcaccgag aacggcatgg cggtggcaac aggatcgtgg gactccttct tgcgtgtatg
     3961 gaactaaaaa taaaacagaa acagaaacag cagcataagc agaaacagaa acaaaacaaa
     4021 acaaaaaaca agagaaaacc aaacaaacaa agaacaacaa aaaaaagagg aaaaagaaaa
     4081 agaacttgaa aatgcattaa agggtggggt ggggtgtcag gtcaactgtt gcgtcagtct
     4141 gcggatagtg tttcacccat catctacatt gtcatctaat gtatatgatt cggtgttacc
     4201 gtctctgtgt gaaatattaa atgagttgcc ggatcgtttt gaatatgtgg gcgtcacgta
     4261 gttgactgaa cgtagaccac aacaacaaag aactttatat acaacattat tttatattgc
     4321 tatatactta ttaactaaaa attatacaaa aaaaacaaaa taaaaccaaa aaaaaaaaac
     4381 aacaaaaacc gaaaaaatcg cagcaacata caaatagaca tacacacgca tacaagaata
     4441 ttattagtgt aaatggaaaa ttgcagaaaa gaaaaaatga aaaaccaaga acaaccgatc
     4501 aagaagaaac aaaacgataa agaaatgatg gagtaacgat gggcccgctg atagtcagaa
     4561 gagtaagagt cagttgttta gtcagaattc
//