From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human cellular c-Ki-ras2 proto-oncogene, 5' flank and exon 0.
Date: 31 Jul 92 10:01:33 GMT
Distribution: bionet
Checksum: 04815     5

LOCUS       HUMRASK01     741 bp ds-DNA             PRI       31-JUL-1992
DEFINITION  Human cellular c-Ki-ras2 proto-oncogene, 5' flank and exon 0.
ACCESSION   L00044 K00653
KEYWORDS    K-ras oncogene; c-myc oncogene; proto-oncogene; ras oncogene;
            transforming gene; viral homology.
SEGMENT     1 of 6
SOURCE      Homo sapiens (library: Lawn et al.) DNA; and Homo sapiens colon and
            lung cDNA to mRNA.
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 741)
  AUTHORS   McGrath,J.P., Capon,D.J., Smith,D.H., Chen,E.Y., Seeburg,P.H.,
            Goeddel,D.V. and Levinson,A.D.
  TITLE     Structure and organization of the human Ki-ras proto-oncogene and a
            related processed pseudogene
  JOURNAL   Nature 304, 501-506 (1983)
  STANDARD  full staff_review
REFERENCE   2  (bases 410 to 579)
  AUTHORS   Capon,D.J., Seeburg,P.H., McGrath,J.P., Hayflick,J.S., Edman,U.,
            Levinson,A.D. and Goeddel,D.V.
  TITLE     Activation of Ki-ras2 gene in human colon and lung carcinomas by
            two different point mutations
  JOURNAL   Nature 304, 507-513 (1983)
  STANDARD  full staff_review
COMMENT     The human genome contains two seperate homologues to the Kirsten
            murine sarcoma virus p21 gene (v-Ki-ras). One (c-Ki-ras1) appears
            to be a processed pseudogene of the other, functional gene
            (c-Ki-ras2).
            The c-Ki-ras2 gene of Calu-1 and PR371 lung carcinoma cells, and
            SW480 colon carcinoma cells all differ from the wild type c-Ki-ras2
            found in normal human cell lines at codon 12 (bases 129-131 in this
            sequence). Substitution of either cysteine or valine for the normal
            glycine at this position in the amino acid sequence seems to be
            sufficient to cause transformation. The lung carcinoma cell line
            PR310 contains no mutation at codon 12 and is not able to induce
            transformation in NIH-3T3 cells.
            The functional c-Ki-ras2 gene contains six exons spanning 35-40 kbp
            in the human genome. The exons have been named 0, 1, 2, 3, 4a and
            4b because the coding region begins in the second exon and either
            exon 4a or 4b is spliced onto exon 3 during mRNA processing. Thus
            the c-Ki-ras2 gene can code for two highly related but distinct
            proteins of MW 21,000; both containing the same amino acid sequence
            for the N-terminal 150 residues (encoded by exons 1-3), but
            followed by either the 39 amino acids encoded by exon 4a or the 38
            amino acids encoded by exon 4b.
            [1] used two probes (one specific for exon 4a, the other for 4b) to
            determine the percentage of mRNAs containing the 4b sequence. They
            found that in cell line SW480 about 99% of the mRNAs contained a
            1-2-3-4b sequence while the remaining 1% contained a 1-2-3-4a-4b
            sequence. For an explanation of splice sites used see segments 5
            and 6 for this entr
FEATURES             Location/Qualifiers
     exon            <540..579
                     /number=0
                     /gene="c-Ki-ras2"
     allele          replace(540,"c")
                     /type="SW480"
                     /gene="c-Ki-ras2"
     allele          replace(540,"a")
                     /type="SW480"
                     /gene="c-Ki-ras2"
BASE COUNT       78 a    295 c    273 g     95 t
ORIGIN      4 bp upstream of SacII site.
        1 ccgcggtccg gtcccgctcc gggtcagaat tggcggctgc ggggacagcc ttgcggctag
       61 gcagggggcg ggccgccgcg tgggtccggc agtccctcct cccgccaagg cgccgcccag
      121 acccgctctc cagccggccc ggctcgccac cctagacccc cccagccacc ccttcctccg
      181 ccggcccggc ccccgctcct cccccgccgg cccggcccgg ccccctcctt ctccccgccg
      241 gcgctcgctg cctccccctc ttccctcttc ccacaccgcc ctcagccgct ccctctcgta
      301 cgcccgtctg aagaagaatc gagcgcggaa cgcatcgata gctctgccct ctgcggccgc
      361 ccggccccga actcatcggt gtgctcggag ctcgattttc ctaggcggcg gccgcggcgg
      421 cggaggcagc agcggcggcg gcagtggcgg cggcgaaggt ggcggcggct cggccagtac
      481 tcccggcccc cgccatttcg gactgggagc gagcgcggcg caggcactga aggcggcggc
      541 ggggccagag gctcagcggc tcccaggtgc gggagagagg tacggagcgg accacccctc
      601 ctgggcccct gcccgggtcc cgaccctctt tgccggcgcc gggcggggcc ggcggcgagt
      661 gaatgaatta ggggtccccg gaggggcggg tggggggcgc gggcgcgggg tcggggcggg
      721 ctgggtgaga ggggtctgca g
//