From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Homo sapiens apurinic/apyrimidinic endonuclease (HAP1) gene,
Date: 21 Aug 92 10:11:35 GMT
Distribution: bionet
Checksum: 51036     7

LOCUS       HUMHAP       3046 bp ds-DNA             PRI       21-AUG-1992
DEFINITION  Homo sapiens apurinic/apyrimidinic endonuclease (HAP1) gene,
            complete cds.
ACCESSION   M92444
KEYWORDS    apurinic/apyrimidinic endonuclease; endonuclease.
SOURCE      Homo sapiens (library: Lambda HAP-8, EMBL-3, Clontech Cat HL1006d)
            DNA.
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 3046)
  AUTHORS   Zhao,B., Grandy,D.K., Hagerup,J.M., Magenis,R.E., Smith,L.,
            Chauhan,B.C. and Henner,W.D.
  TITLE     The human gene for apurinic/apyrimidinic endonuclease (HAP1):
            sequence and localisation to chromosome 14 band q12
  JOURNAL   Nucleic Acids Res. 20, 4097-4098 (1992)
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     CDS             join(848..905,1116..1303,1870..2062,2193..2710)
                     /function="'DNA repair enzyme'"
                     /EC_number="3.1.25.2"
                     /product="apurinic/apyrimidinic endonuclease"
                     /gene="HAP1"
                     /label=HAP1_protein
                     /codon_start=1
                     /translation="MPKRGKKGAVAEDGDELRTEPEAKKSKTAAKKNDKEAAGEGPAL
                     YEDPPDQKTSPSGKPATLKICSWNVDGLRAWIKKKGLDWVKEEAPDILCLQETKCSEN
                     KLPAELQELPGLSHQYWSAPSDKEGYSGVGLLSRQCPLKVSYGIGDEEHDQEGRVIVA
                     EFDSFVLVTAYVPNAGRGLVRLEYRQRWDEAFRKFLKGLASRKPLVLCGDLNVAHEEI
                     DLRNPKGNKKNAGFTPQERQGFGELLQAVPLADSFRHLYPNTPYAYTFWTYMMNARSK
                     NVGWRLDYFLLSHSLLPALCDSKIRSKALGSDHCPITLYLAL"
     mRNA            join(439..596,780..905,1116..1303,1870..2062,2193..2968)
                     /gene="HAP1"
     exon            439..596
                     /note="'determined by primer extension from primer 178'"
                     /note="1"
                     /gene="HAP1"
     primer_bind     complement(526..550)
                     /gene="HAP1"
                     /label=primer_178
     intron          597..779
                     /number=1
                     /gene="HAP1"
     exon            780..905
                     /number=2
                     /note="2"
                     /gene="HAP1"
     intron          906..1115
                     /number=2
                     /gene="HAP1"
     exon            1116..1303
                     /number=3
                     /gene="HAP1"
     intron          1304..1869
                     /number=3
                     /gene="HAP1"
     exon            1870..2062
                     /note="4"
                     /gene="HAP1"
     intron          2063..2192
                     /number=4
                     /gene="HAP1"
     exon            2193..2968
                     /number=5
                     /gene="HAP1"
BASE COUNT      728 a    739 c    729 g    850 t
ORIGIN
        1 ctgcagatag cactgggaaa gacaccgcgg aactccagcg agcggagaca cgccaaggcc
       61 cctccaggga cctgtcttcc taactgccag ggacgccgag ccaactctgt gccttacatt
      121 cgtatccgtt ttcctatctc tttcccgtgg tccagcccag ccttctccac tgtttttttc
      181 cctcttgcac agagttagaa tcttaagtca gtgtcacaca atgtgctgtg catctggcac
      241 aacgataaac agcccgaggg agggttgggg acctaagtgt cctagagaat tagaggaggg
      301 aggcgaggct aagcgtctcc gtcacgtggt gtcagacaga ccaatcacgc gcattcttcg
      361 gccacgacaa gcgcgcctct gatcacgtga ccaggtccgc tacccacgtg ggggctcagc
      421 gtgcaccctt ctttgtgctc gggttaggag gagctaggct gccatcgggc cggtgcagat
      481 acggggttgc tcttttgctc ataagagggg cttcgctggc agtctgaacg gcaagcttga
      541 gtcaggaccc ttaattaaga tcctcaattg gctggagggc agatctcgcg agtagggtac
      601 aaggcactat gaaatgatct agtttcgtgg gtgaggggct gaagggccta tgatgcacgg
      661 aggcggggaa aggatttaga gataacgtgg tttgaaaggc gggacctggt gcggggacgc
      721 tcttgggagg agtcttctcc ccagccttag ctggtttcat gatttctttg cgtctgtagg
      781 caacgcggta aaaatattgc ttcggtgggt gacgcggtac agctgcccaa gggcgttcgt
      841 aacgggaatg ccgaagcgtg ggaaaaaggg agcggtggcg gaagacgggg atgagctcag
      901 gacaggtaag ggaatgaaat cagcccttct tcctagaagc tgcggcgggg gtgtttgtca
      961 ttcccttgat gtacggtaag tacgggccga ctcatttttg caggggtttg tgaagaagtc
     1021 gcaggaaccg taggctttcg ttgggtctat agttaacgcc ggatcgcagt tggaaaccac
     1081 cagctttttg tcagtatata ttactcattt tatagagcca gaggccaaga agagtaagac
     1141 ggccgcaaag aaaaatgaca aagaggcagc aggagagggc ccagccctgt atgaggaccc
     1201 cccagatcag aaaacctcac ccagtggcaa acctgccaca ctcaagatct gctcttggaa
     1261 tgtggatggg cttcgagcct ggattaagaa gaaaggatta gatgtgagtg gaatttgagg
     1321 gaaagagaca ttttttagta ttgaatggtc ttagggttta gtcacccctt ttctccgttt
     1381 agccttcagg ctgttttatt tttctcctgc ccgtagtttt ctgtggggct tccccagtct
     1441 tgccagttgt atttcctaaa tgtctgttcc ttcacttcca ttgccatttt cttttttagt
     1501 gttctctcct cttcccagaa tgttgcaaaa acctcttcac tatacttcct ccattttatc
     1561 ttcctgcatt gcattccata tgaagcatgt cctccattcc attaaccata gcttaaaaat
     1621 cttagcttgc tatccactgc ctatagaaaa aacacatctc cttggcatag catgtaagac
     1681 tttcttacct ctctatattt gttttcattt atctagctta gaattgtttg aatattgtgc
     1741 tgcttgactc gaactcctta ggccaagaga ctgtttaacc cgtgcgtatc tatgacttag
     1801 catatagatt attcaataaa tgttctgctg aattgataat acgttttcca cctttctttt
     1861 cacttacagt gggtaaagga agaagcccca gatatactgt gccttcaaga gaccaaatgt
     1921 tcagagaaca aactaccagc tgaacttcag gagctgcctg gactctctca tcaatactgg
     1981 tcagctcctt cggacaagga agggtacagt ggcgtgggcc tgctttcccg ccagtgccca
     2041 ctcaaagttt cttacggcat aggtgagacc ctattgatgc ctaatgcctg aactcttcaa
     2101 aaccaattgc taattctcta tctctgcccc acctcttgat tgctttccct tttcttatag
     2161 ttttttatgc taattctgtt tcatttctat aggcgatgag gagcatgatc aggaaggccg
     2221 ggtgattgtg gctgaatttg actcgtttgt gctggtaaca gcatatgtac ctaatgcagg
     2281 ccgaggtctg gtacgactgg agtaccggca gcgctgggat gaagcctttc gcaagttcct
     2341 gaagggcctg gcttcccgaa agccccttgt gctgtgtgga gacctcaatg tggcacatga
     2401 agaaattgac cttcgcaacc ccaaggggaa caaaaagaat gctggcttca cgccacaaga
     2461 gcgccaaggc ttcggggaat tactgcaggc tgtgccactg gctgacagct ttaggcacct
     2521 ctaccccaac acaccctatg cctacacctt ttggacttat atgatgaatg ctcgatccaa
     2581 gaatgttggt tggcgccttg attacttttt gttgtcccac tctctgttac ctgcattgtg
     2641 tgacagcaag atccgttcca aggccctcgg cagtgatcac tgtcctatca ccctatacct
     2701 agcactgtga caccacccct aaatcacttt gagcctggga aataagcccc ctcaactacc
     2761 attccttctt taaacactct tcagagaaat ctgcattcta tttctcatgt ataaaactag
     2821 gaatcctcca accaggctcc tgtgatagag ttcttttaag cccaagattt tttatttgag
     2881 ggttttttgt tttttaaaaa aaaattgaac aaagactact aatgactttg tttgaattat
     2941 ccacatgaaa ataaagagcc atagtttcag ccttgctgtc ttcgtgtctt accccttcgt
     3001 ggggctacac attctcttcc tcatattttc atgcacacaa gttgac
//