From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Bacillus subtilis modifier protein of major autolysin (cwbA) gene
Date: 12 Sep 92 10:01:11 GMT
Distribution: bionet
Checksum: 62962    11

LOCUS       BACCWBA      5198 bp ds-DNA             BCT       12-SEP-1992
DEFINITION  Bacillus subtilis modifier protein of major autolysin (cwbA) gene
            and assumed lipoprotein (lppX) gene, complete cds.
ACCESSION   D10388
KEYWORDS    lipoprotein; major autolysin.
SOURCE      Bacillus subtilis (strain 168S) DNA.
  ORGANISM  Bacillus subtilis
            Prokaryotae; Firmicutes; Endospore-forming rods and cocci;
            Bacillaceae.
REFERENCE   1  (sites)
  AUTHORS   Kuroda,A. and Sekiguchi,J.
  TITLE     Molecular cloning and sequencing of a major Bacillus subtilis
            autolysin gene
  JOURNAL   J. Bacteriol. 173, 7304-7312 (1991)
  STANDARD  full staff_review
REFERENCE   2  (bases 1 to 5198)
  AUTHORS   Kuroda,A., Rashid,H.M. and Sekiguchi,J.
  TITLE     Molecular cloning and sequencing of the upstream region of the
            major Bacillus subtilis autolysin gene: a modifier protein
            exhibiting sequence homology to the major autolysin and the spoIID
            product
  JOURNAL   J. Gen. Microbiol. 138, 1067-1076 (1992)
  STANDARD  full staff_review
COMMENT     Submitted (29-Jan-1992) to DDBJ by:
            Junichi Sekiguchi
            Department of Applied Biology
            Faculty of Textile Science and Technology
            Shinshu University
            3-15-1 Tokida, Ueda-shi
            Nagano 386
            Japan
            Phone:  0268-22-1215
            Fax:    0268-22-4079
            [1]  sites.
FEATURES             Location/Qualifiers
     -35_signal      908..911
     -10_signal      927..934
     RBS             943..949
     CDS             955..1263
                     /product="lipoprotein"
                     /gene="lppX"
                     /codon_start=1
                     /translation="MKKFIALLFFILLLSGCGVNSQKSQGEDVSPDSNIETKEGTYVG
                     LADTHTIEVTVDNEPVSLDITEESTSDLDKFNSGDKVTITYEKNDEGQLLLKDIERAN
                     "
     sig_peptide     955..1002
                     /codon_start=1
     mat_peptide     1003..1260
                     /product="lipoprotein"
                     /gene="lppX"
                     /codon_start=1
     RBS             1269..1275
     CDS             1287..3404
                     /product="modifier protein of major autolysin"
                     /gene="cwbA"
                     /codon_start=1
                     /translation="MKSCKQLIVCSLAAILLLIPSVSFAADSNISVKLLNYIGNKSSI
                     SLSPTGFYKVTGDNVAVTDRFAGASRYETATLASNSQWKNPNTVILVNRDIFIDALPV
                     IPLAKKLNAPVLFTQPDTLTKTTERQIAKFNPDNILIIGGARSISKDVENKLKSYGAV
                     KRISGKNRYVLSENIAKQMGSYDKAIVVTGRVFQDALAIAPYAAAHGYPILLTEKDKL
                     PDYDLPKQVIIIGSSFSVSDSVENQIKKTSTVQRIPGSTRYELTANIIKQLKLKADKV
                     VMTNGTKYADVLIGASLASKKNSQILFVKQDSVPAAAKSITKDKATYAYDFIGSTSSI
                     SAEVENSLADEFYLADGGTYNLKINSGKLNLENIKTYGNSLRIKPENYSTSNRISLDG
                     KQYLGTVNFSIESTKYIRPVNENIPFEDYLKGVIPNEMPASWSLEALKAQTVAARTYS
                     ITKTGTTVPDTTAFQVYGGYSWNSNTNKAVEQTKGKVLKYNGSLITAAYSSSNGGYTE
                     ASNEVWSSSVPYLIAKKDTKDPQIGWTLTLSKQQLDTKSLDLTKPSSWWSSATETDSA
                     RLSGVKNWILKNKETSADSVKIASIDDLSFSGTTQGQRAKTASMKVKYFVKSSTGSYN
                     LSKITTISVPTSELRTMIGATVFKSTYVTVKKDTSKYTISGKGYGHGIGMSQYGAKAR
                     AEAGDSYSSILKFYYPGTTLTSY"
     sig_peptide     1287..1361
                     /codon_start=1
     mat_peptide     1362..3401
                     /product="modifier protein of major autolysin"
                     /gene="cwbA"
                     /codon_start=1
     CDS             3443..4933
                     /EC_number="3.5.1.28"
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /gene="cwlB"
                     /codon_start=1
                     /translation="MRSYIKVLTMCFLGLILFVPTALADNSVKRVGGSNRYGTAVQIS
                     KQMYSTASTAVIVGGSSYADAISAAPLAYQKNAPLLYTNSDKLSYETKTRLKEMQTKN
                     VIIVGGTPAVSSNTANQIKSLGISIKRIAGSNRYDTAARVAKAMGATSKAVILNGFLY
                     ADAPAVIPYAAKNGYPILFTNKTSINSATTSVIKDKGISSTVVVGGTGSISNTVYNKL
                     PSPTRISGSNRYELAANIVQKLNLSTSTVYVSNGFSYPDSIAGATLAAKKKQSLILTN
                     GENLSTGARKIIGSKNMSNFMIIGNTPAVSTKVANQLKNPVVGETIFIDPGHGDQDSG
                     AIGNGLLEKEVNLDIAKRVNTKLNASGALPVLSRSNDTFYSLQERVNKAASAQADLFL
                     SIHANANDSSSPNGSETYYDTTYQAANSKRLAEQIQPKLAANLGTRDRGVKTAAFYVI
                     KYSKMPSVLVETAFITNASDASKLKQAVYKDKAAQAIHDGTVSYYR"
BASE COUNT     1750 a    939 c   1020 g   1489 t
ORIGIN
        1 gatatcccac atgttatcga aggtcaagtt cgttttcaca ttattttcaa caactttaaa
       61 catatctccg aatttcgtga tggaagaaat attagcgcct ttattaataa taccttggat
      121 gacttggcgc tggcggtcct ggcgtccgaa gtcgcctctc ggatcttctt ttctcatccg
      181 ggtataagcg agcgcttctt ttccgttcag cgtgatttct cctttgccga acgaataacc
      241 gtcataactg aacgcgaatg tgctgttaac tgtaatgccg ccaagtgtat caacaacgtc
      301 tctaaagctt tccatgttca ctttcacaaa ataatcaacc ggaacatcga ggaagttctc
      361 aacagtgtct accgtcatct gtgtgccgcc gaaagcgtat gaatggttga ttttatccat
      421 tgtgcctttt ccgataattt ttgtatacgt gtcacgcgga atgctgacca tgtcggttgt
      481 attcgttttt gggttaacgg tcatataaat gagcgtatcg gcacggccct tatcgccgtc
      541 gcgttcatcc acacccatga ttaagacgga aaaaggatct tttttattga tactgacctc
      601 tttatccctc tttttagatt tatcaatgct ttcatgaata cttgccacgg ttgaagctgc
      661 tttatgccat aagtagtatg catagccgcc agttccgagt acaagaagtc cgattatcgt
      721 taaaattgtc agtaataaag tttttttctt ttttctgcgt tcgtttctca tcctttgcac
      781 ctcgtctgtt aaattacttt cattatgagt taaatttcca ataaatacaa ctctatttaa
      841 tacaagaatg aaatcctaaa atagtcgttt tttataaaaa aaagtttcat tgtttcttaa
      901 tttttcttta aaatataaaa taggttgacg ataaaatata atgaggtgaa aaaaatgaaa
      961 aaatttattg ctttactgtt ctttatattg cttctttcgg gttgcggggt taatagtcaa
     1021 aagagtcaag gtgaagatgt atcgccagac agtaacattg aaacaaaaga aggtacttat
     1081 gtagggttag ctgatactca tacaatagaa gtaacagtag ataatgagcc ggttagtctt
     1141 gatatcactg aagaatcgac aagtgatctt gataagttta acagtggaga taaggtcacg
     1201 attacatatg aaaaaaatga tgagggtcag cttctgttaa aagatattga acgtgccaac
     1261 taagatatag ggaggaacct gaaattttga aatcttgcaa acaattgata gtgtgttcac
     1321 ttgctgcaat cttattattg attccatcag tttcttttgc agcagactca aacatctcag
     1381 ttaaattgtt aaattatatt ggaaataaat ctagcatcag tctatcgcca acaggatttt
     1441 ataaagtaac tggagataat gttgctgtta cggatcgttt tgcaggcgct tcacgatacg
     1501 aaactgcgac tcttgcatcc aacagccagt ggaaaaatcc taatacagtg atattggtga
     1561 accgagatat ttttattgat gcattgcctg ttattccatt ggcgaaaaaa ctgaacgcac
     1621 cagttttatt tactcagcct gacacgttaa caaaaactac tgaaagacaa attgctaagt
     1681 ttaaccctga taatatttta atcattgggg gagcgcgaag catttcaaaa gacgtagaaa
     1741 acaagctgaa atcatatggc gctgtgaaaa ggataagcgg aaaaaataga tatgtcttat
     1801 cagaaaatat tgcaaagcaa atgggcagct atgataaagc gatcgttgta accggaagag
     1861 tatttcaaga cgctttagcc attgctccat atgcagcagc acacggatac cctattttgc
     1921 ttacagagaa agacaagctt ccggattatg atttgccaaa acaagtcatc attataggca
     1981 gttcatttag cgtcagtgat agtgtagaaa accaaattaa aaaaacttca actgtgcaaa
     2041 ggattcctgg atcaaccaga tatgaactta cggcaaatat aattaagcaa ctaaagttga
     2101 aggctgataa agtcgtaatg acgaacggga caaaatatgc agacgtattg ataggcgctt
     2161 cgcttgcctc gaagaaaaac agccaaattt tatttgttaa acaagatagc gttccggctg
     2221 cggctaaaag tataacgaaa gataaagcca catatgcata cgactttatt ggaagtacca
     2281 gttcaatttc tgctgaagtt gagaattcac ttgctgatga attttattta gctgacggtg
     2341 gcacctataa tctaaaaatc aacagcggaa aattaaacct tgaaaacatc aaaacttacg
     2401 gaaactccct aagaattaaa ccagaaaact attctacttc aaacagaatt agtcttgatg
     2461 gaaagcagta tcttggcacg gttaatttct ctattgagtc tactaaatat atccgaccgg
     2521 taaatgagaa tattccattt gaggattact taaaaggagt aattccgaat gaaatgcctg
     2581 ctagctggtc acttgaggct cttaaagcac agactgtcgc tgcaagaacc tactcaataa
     2641 caaaaacagg aactactgta cctgatacaa ccgcttttca agtttatggt ggctacagtt
     2701 ggaattcgaa taccaataaa gcggtagagc aaacgaaggg gaaagttctg aaatataatg
     2761 gatctcttat aactgcagct tattcttcca gtaatggtgg ttacacagaa gctagtaatg
     2821 aagtgtggtc gtcaagtgta ccttacttga ttgcaaaaaa agatacaaag gatcctcaaa
     2881 taggctggac gcttactttg tcaaaacaac agctagatac gaaatctttg gacttaacga
     2941 agccttcttc ttggtggtct tcagcgactg aaactgattc tgcaagactt tccggagtta
     3001 agaactggat cttgaaaaat aaagaaacaa gcgctgattc tgtcaaaatt gcaagtattg
     3061 acgatttaag ctttagtgga acgacacaag ggcagcgtgc aaaaacagca tcgatgaaag
     3121 tgaagtattt tgttaaaagc agtactggct cctataactt gagcaaaatt acaaccatta
     3181 gtgtaccaac aagtgagtta agaacaatga ttggagcgac tgtatttaaa agtacttatg
     3241 tgactgtaaa aaaagatact tcaaagtaca caatcagcgg taaaggatat ggtcacggga
     3301 tcggaatgag tcaatatgga gcaaaagcaa gagcagaagc tggagattca tatagttcaa
     3361 ttttgaaatt ttactatcca ggtacaactc tgacaagtta ttgattattt taggatataa
     3421 cgccatttaa ggaggaaatc aattgcgttc ttatataaaa gtcctaacaa tgtgttttct
     3481 ggggctcata ctttttgtgc caacagcttt ggccgataac tcagtgaaaa gagttggggg
     3541 aagcaataga tacggcactg ctgtacaaat atcaaagcaa atgtattcaa cagcaagtac
     3601 agctgtaatt gttggtggga gttcctatgc agatgctatt tcagcagcac ctcttgctta
     3661 ccagaagaat gcgccattgc tttacactaa ttctgataag ctttcatatg aaacgaaaac
     3721 aagattgaaa gagatgcaga ctaaaaatgt aattattgta ggcggaacac ctgctgtttc
     3781 ttctaacact gctaaccaga ttaaaagctt ggggataagt attaaacgaa ttgcaggaag
     3841 caaccgttat gatacggctg cacgggtggc aaaagcgatg ggtgcgactt caaaagctgt
     3901 tattttgaac ggcttcttat atgcagacgc tccggccgtc atcccttatg cagcgaaaaa
     3961 cgggtatcca attcttttta caaataaaac atctataaat agtgcgacta cgtctgtgat
     4021 aaaagataag ggaatttcga gtaccgttgt tgtaggaggc actggaagta tcagcaatac
     4081 ggtatacaac aagttacctt ctcctacaag aattagcggt tcaaacagat atgagcttgc
     4141 tgcaaatatc gtacaaaaac ttaatttatc aacaagcacc gtatatgtaa gcaatggatt
     4201 cagctaccct gactctattg caggagctac actggcagct aagaagaagc aatctcttat
     4261 tcttacaaat ggtgaaaatt tatctacagg agcccgtaaa attattggaa gtaaaaacat
     4321 gtcaaacttt atgattatcg gaaacactcc tgccgtaagc acaaaggttg ctaatcagct
     4381 aaagaatcca gttgtaggtg aaacaatctt tattgatccg ggtcacggtg atcaagattc
     4441 aggagcaatc ggcaatggac tccttgagaa agaagtcaac cttgatatag cgaaaagagt
     4501 caatacaaag ctaaatgctt caggtgctct tccagtactg tcaagatcta atgatacttt
     4561 ttattcttta caggagagag taaataaagc agcttctgca caagcagatt tatttctcag
     4621 tatacatgca aatgctaatg atagctcatc accaaatgga agtgagacgt actacgatac
     4681 aacatatcaa gctgcaaata gcaagagact ggctgaacaa attcaaccaa agttagcggc
     4741 taatcttgga acgagagacc ggggagtaaa aacagctgct ttctatgtta ttaaatattc
     4801 taaaatgccg agtgttttag ttgaaactgc ctttatcact aatgcatcag atgcaagtaa
     4861 attgaagcaa gcggtttata aagataaagc tgcacaagct attcatgacg gcacagtatc
     4921 ttattacaga taatcgaaag agacaaatct aatcacagat ttgtctcttt tttatatgaa
     4981 atgattaaaa caaataggac ttgtatataa aatagtaaaa gagtattaac aacatttatc
     5041 agaaaaaaca cgtaattcac acttcttaac ataccattta catccaatta acatccgtct
     5101 gctaaactga ctggcatagg tcatcataac atcccaaaca aatcgtgagg tgtgtgtcag
     5161 tgagtgcaga gaaaagcatg aatgtgagtc gtgaattc
//