From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Homo sapiens (clone lamda-hPEC-3) phosphoenolpyruvate carboxykinase
Date: 22 Dec 92 22:54:10 GMT
Distribution: bionet
Checksum: 56435 6

LOCUS       HUMPHOCAR    2657 bp ss-mRNA            PRI       30-NOV-1992
DEFINITION  Homo sapiens (clone lamda-hPEC-3) phosphoenolpyruvate carboxykinase
            (PCK1) mRNA, complete cds.
ACCESSION   L05144
KEYWORDS    phosphoenolpyruvate carboxykinase.
SOURCE      Homo sapiens adult kidney cDNA to mRNA.
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 2657)
  AUTHORS   Stoffel,M., Xiang,K.-s., Espinosa,R.III., Cox,N.J., Le Beau,M.M.
            and Bell,G.I.
  TITLE     cDNA sequence and localization of polymorphic human cytosolic
            phosphoenolpyruvate carboxykinase gene (PCK1) to chromosome 20,
            band q13.3: PCK1 is not tightly linked to maturity-onset diabetes
            of the young
  JOURNAL   Unpublished (1993)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     repeat_region   2362..2403
                     /gene="PCK1"
     CDS             122..1990
                     /EC_number="4.1.1.32"
                     /gene="PCK1"
                     /product="phosphoenolpyruvate carboxykinase"
                     /codon_start=1
                     /translation="MPPQLQNGLNLSAKVVQGSLDSLPQAVREFLENNAELCQPDHIH
                     ICDGSEEENGRLLGQMEEEGILRRLKKYDNCWLALTDPRDVARIESKTVIVTQEQRDT
                     VPIPKTGLSQLGRWMSEEDFEKAFNARFPGCMKGRTMYVIPFSMGPLGSPLSKIGIEL
                     TDSPYVVASMRIMTRMGTPVLEALGDGEFVKCLHSVGCPLPLQKPLVNNWPCNPELTL
                     IAHLPDRREIISFGSGYGGNSLLGKKCFALRMASRLAEEEGWLAEHMLILGITNPEGE
                     KKYLAAAFPSACGKTNLAMMNPSLPGWKVECVGDDIAWMKFDAQGHLRAINPENGFFG
                     VAPGTSVKTNPNAIKTIQKNTIFTNVAETSDGGVYWEGIDEPLASGVTITSWKNKEWS
                     SEDGEPCAHPNSRFCTPASQCPIIDAAWESPEGVPIEGIIFGGRRPAGVPLVYEALSW
                     QHGVFVGAAMRSEATAAAEHKGKIIMHDPFAMRPFFGYNFGKYLAHWLSMAQHPAAKL
                     PKIFHVNWFRKDKEGKFLWPGFGENSRVLEWMFNRIDGKASTNVTPIGYIPKEDALNL
                     KGLGHINMMELFSISKEFWDKEVEDIEKYLVDQVNADLPCEIEREILALKQRISQM"
BASE COUNT      695 a    644 c    707 g    611 t
ORIGIN      
        1 tgggaacaca aacttgctgg cgggaagagc ccggaaagaa acctgtggat ctcccttcga
       61 gatcatccaa agagaagaaa ggtgacctca cattcgtgcc ccttagcagc actctgcaga
      121 aatgcctcct cagctgcaaa acggcctgaa cctctcggcc aaagttgtcc agggaagcct
      181 ggacagcctg ccccaggcag tgagggagtt tctcgagaat aacgctgagc tgtgtcagcc
      241 tgatcacatc cacatctgtg acggctctga ggaggagaat gggcggcttc tgggccagat
      301 ggaggaagag ggcatcctca ggcggctgaa gaagtatgac aactgctggt tggctctcac
      361 tgaccccagg gatgtggcca ggatcgaaag caagacggtt atcgtcaccc aagagcaaag
      421 agacacagtg cccatcccca aaacaggcct cagccagctc ggtcgctgga tgtcagagga
      481 ggattttgag aaagcgttca atgccaggtt cccagggtgc atgaaaggtc gcaccatgta
      541 cgtcatccca ttcagcatgg ggccgctggg ctcacctctg tcgaagatcg gcatcgagct
      601 gacggattcg ccctacgtgg tggccagcat gcggatcatg acgcggatgg gcacgcccgt
      661 cctggaagca ctgggcgatg gggagtttgt caaatgcctc cattctgtgg ggtgccctct
      721 gcctttacaa aagcctttgg tcaacaactg gccctgcaac ccggagctga cgctcatcgc
      781 ccacctgcct gaccgcagag agatcatctc ctttggcagt gggtacggcg ggaactcgct
      841 gctcgggaag aagtgctttg ctctcaggat ggccagccgg ctggcagagg aggaagggtg
      901 gctggcagag cacatgctga ttctgggtat aaccaaccct gagggtgaga agaagtacct
      961 ggcggccgca tttcccagcg cctgcgggaa gaccaacctg gccatgatga accccagcct
     1021 ccccgggtgg aaggttgagt gcgtcgggga tgacattgcc tggatgaagt ttgacgcaca
     1081 aggtcattta agggccatca acccagaaaa tggctttttc ggtgtcgctc ctgggacttc
     1141 agtgaagacc aaccccaatg ccatcaagac catccagaag aacacaatct ttaccaatgt
     1201 ggccgagacc agcgacgggg gcgtttactg ggaaggcatt gatgagccgc tagcttcagg
     1261 cgtcaccatc acgtcctgga agaataagga gtggagctca gaggatgggg aaccttgtgc
     1321 ccaccccaac tcgaggttct gcacccctgc cagccagtgc cccatcattg atgctgcctg
     1381 ggagtctccg gaaggtgttc ccattgaagg cattatcttt ggaggccgta gacctgctgg
     1441 tgtccctcta gtctatgaag ctctcagctg gcaacatgga gtctttgtgg gggcggccat
     1501 gagatcagag gccacagcgg ctgcagaaca taaaggcaaa atcatcatgc atgacccctt
     1561 tgccatgcgg cccttctttg gctacaactt cggcaaatac ctggcccact ggcttagcat
     1621 ggcccagcac ccagcagcca aactgcccaa gatcttccat gtcaactggt tccggaagga
     1681 caaggaaggc aaattcctct ggccaggctt tggagagaac tccagggtgc tggagtggat
     1741 gttcaaccgg atcgatggaa aagccagcac caacgtcacg cccataggct acatccccaa
     1801 ggaggatgcc ctgaacctga aaggcctggg gcacatcaac atgatggagc ttttcagcat
     1861 ctccaaggaa ttctgggaca aggaggtgga agacatcgag aagtatctgg tggatcaagt
     1921 caatgccgac ctcccctgtg aaatcgagag agagatcctt gccttgaagc aaagaataag
     1981 ccagatgtaa tcagggcctg agaataagcc agatgtaatc agggcctgag tgctttacct
     2041 ttaaaatcat taaattaaaa tccataaggt gcagtaggag caagagaggg caagtgttcc
     2101 caaattgacg ccacctaata atcatcacca caccgggagc agatctgaag gcacactttg
     2161 atttttttaa ggataagaac cacagaacac tgggtagtag ctaatgaaat tgagaaggga
     2221 aatcttagca tgcctccaaa aattcacatc caatgcatac tttgttcaaa tttaaggtta
     2281 ctcaggcatt gatcttttca gtgttttttc acttagctat gtggattagc tagaatgcac
     2341 accaaaaaga tacttgagct gtatatatat atgtgtgtgt gtgtgtgtgt gtgtgtgtgt
     2401 gtgcatgtat gtgcacatgt gtctgtgtga tatttggtat gtgtatttgt atgtactgtt
     2461 attcaaaata tatttaatac ctttggaaaa tcttgggcaa gatgacctac tagttttcct
     2521 tgaaaaaaag ttgctttgtt attaatattg tgcttaaatt atttttatac accattgttc
     2581 cttaccttta cataattgca atatttcccc cttactactt cttggaaaaa aattagaaaa
     2641 tgaagtttat agaaaag
//