HDTenEightyP/NetNews huggingface dataset:
From: GIETZ@bldghsc.lan1.umanitoba.ca
Newsgroups: bionet.molbio.methds-reagnts
Subject: Re:Re: DNA purification
Date: 11 Jan 93 19:34:00 GMT
Distribution: bionet

%%%%%%%%%%%%%Stephanie Writes %%%%%%%%%%%%%
%In article <1993Jan11.112657.23798@crc.ac.uk>
%sbhattac@crc.ac.uk (Dr. S. Bhattacharya) writes:
 
>
>Hi all
>I got a few requests to explain this method in more detail so am posting it
>to the net.
>
(Stuff Deleted)
>
>A far cheaper and faster method than various kits on offer.
>
>Best of luck
>
>Shoumo

% I used this method for a while after hearing about it from a friend.  It
% seemed to give me decent recovery of DNA.  However, I could never use
% DNA purified in this way for ligations.  I just would not ligate at all.
% Any ideas?
 
% Stephanie
 %%%%%%%%%%%%%%%%%%%%%%%%%%%%%%
Hi Stephanie
I have have the same problems with DNA purified this way. 
To get around this problem I
purify DNA fragments from Agarose using the Method of
Girvitz et al (1980) Anal.Biochem. 106:492 as described 
in Maniatis, Fritsch and Sambrook (1982) pg 168.
This method inserts a small piece of dialysis membrane fronted 
with 3MM whatman paper (to hold the drop of buffer to the 
membrane when you remove it) into a slit cut in the gel just in
front of your band of interest . you then
 catch your DNA frag  on the Whatman/membrane 
by electrophoresing it  in the correct
direction.  Its an old method 
but works very well giving high yields of DNA that one can do  
 anything with including ligations. There is no need for special
agarose etc... I always do blunt end ligations
with DNA purified this way with no problems.
A few hints:
    1. I use Whatman 3MM paper not DEAE cellulose paper 
        for better yields.
    2.  Avoid spinning your elutions too hard.  This dries out 
        the paper and reduces your yield substantially.  I spin at 
        2500 rpm for 
        the first two spins and then spin hard the final one. Most 
        your DNA comes off on the 1st and 2nd elution.
    3. I add the first elution wash before spinning the paper dry.
        This seems to increase yield.
    4. An overnight incubation at -20 seems to give better EtOH pellets
       ( A 1X g sedimentation) Other wise your pellet is spread over the
        entire outer face of the tube (still recoverable).
    5.  I only do one phenol:choroform extraction.
    6. Use long wave UV to visualize your bands (this is important 
        especially for ligations.)

I have even purified 10 - 12 Kb MboI yeast DNA to make libraries 
with great success.  

If anyone would like more details just drop me a note.

Dan Gietz
U of Manitoba
GIETZ@BLDGHSC.LAN1.UMANITOBA.CA
From: robison1@husc10.harvard.edu (Keith Robison)
Newsgroups: bionet.molbio.methds-reagnts
Subject: Re: Buffer Making Program
Date: 7 Jan 93 04:48:39 GMT
Distribution: bionet

If you wish to set it up for the same buffers all the time,
then any spreadsheet will do (or TK!Solver -- though you can
do much more fun [read frosh chem homework] wit it).

One of the people here does it and is quite happy with the method.

Keith Robison
Harvard University
Department of Cellular & Developmental Biology
Department of Genetics / HHMI

robison@biosun.harvard.edu
From: pouliot@GENETHON.GENETHON.FR (Yannick Pouliot)
Newsgroups: bionet.molbio.methds-reagnts
Subject: Plastic to which DNA sticks?
Date: 11 Jan 93 17:57:44 GMT

Hello fellow Netters.

I'm writing for a colleague (Frederic Lorenzo):

Does anybody remember the name and supplier of this plastic to which
DNA can bind?  This would be for doing an ELISA-type assay using a
digoxigenin-labelled probe.

Thanks,

Yannick Pouliot 
Genethon.
From: mytelka@codon1 (Daniel Mytelka;612 Barker;x2-5110)
Newsgroups: bionet.molbio.methds-reagnts
Subject: Re: DNA purification
Date: 4 Jan 1993 23:19:42 GMT
Organization: University of California
NNTP-Posting-Host: codon1.berkeley.edu

>Geneclean II glassmilk efficiently removes DNA 200 bases or longer from
>agarose gels. Who has tried to isolate shorter (50-200) fragments.


The MERMAID kit is designed for isolating shorter pieces of DNA (it is also
a BIO101 product). I have never used it for isolating DNA directly from
a gel, but I got essentially 100% recovery of a 20mer when precipitating
from solution. It is designed for isolating from gels as well.

Dan Mytelka (mytelka@mendel.berkeley.edu)
From: beynonrj@uxb.liv.ac.uk (Dr. R.J. Beynon)
Newsgroups: bionet.molbio.methds-reagnts
Subject: Buffer programs
Date: 7 Jan 93 12:08:28 GMT
Organization: The University of Liverpool
X-Newsreader: TIN [version 1.1 PL6]

In response to a request for buffer making programs, you might be
interested in my "BufferStack" - a Mac Hypercard file, that gives
buffer recipes. 

More importantly, it makes the correct thermodynamic corrections
for I and T, and also allows you to make up a buffer at one T
and use it at another. You can add your own buffers.

It lets you define ionic strength, and tells you how much NaCl or KCl to add
to give a fixed I. 

It would be easy to kludge this stack to incorporate extra goodies, like
MgC2 etc. 

BufferStack is available by anonftp or gopher from IUBIO ( a great service,
by the way - thanks Don!)

I never have found out whether anyone ever used BufferStack - if you do and
have comments or suggestions, please send me email. I may be writing
an update in the first quarter of this year.

Conrad Halling makde some excellent cosmetic improvements to BufferStack - 
if anyone else changed the stack, could they let me know.

Cheers
Rob Beynon, Proteolysis Research Group, University of Liverpool

Sites listed below - hope this help too


Host fly.bio.indiana.edu

    Location: /molbio/mac
           FILE -rw-r--r--      84568  May 14 1991  buffer.hqx

Host evolution.bchs.uh.edu

    Location: /pub/gene-server/mac
           FILE -rwxr--r--      84448  Sep 16 1991  bufstack.hqx
           FILE -rwxr--r--      84448  Sep 16 1991  mac-bufstack.h

Host sunbcd.weizmann.ac.il

    Location: /pub/software/mac
           FILE -r--r--r--      78238  May 15 1992  bufstack.hqx

Host sunbcd.weizmann.ac.il

    Location: /pub/software/mac
           FILE -r--r--r--      84568  May 14 1991  buffer.hqx

(not sure whether these are the same - suspect they are??)


Host sunsite.unc.edu

    Location: /pub/academic/biology/molbio/mac
           FILE -r--r--r--      84568  May 14 1991  buffer.hqx
From: mjhf@troi.cc.rochester.edu (Michael James Horsfall)
Newsgroups: bionet.molbio.methds-reagnts
Subject: Re: mutS and recA protein
Date: 6 Jan 93 06:29:06 GMT
Distribution: bionet
Organization: University of Rochester - Rochester, New York

In <33308.jdboer@sol.uvic.ca> jdboer@SOL.UVIC.CA writes:


>I am trying to find some information about the MutS and RecA proteins.
>Specifically such things as DNA binding parameters, effects of buffer, temp,
>etc in in vitro assays, mode of action, etc. A quick survey of the
>literature did not yield to many useful things. Is anyone aware of some
>relevant articles, reviews or otherwise good source of information?

Hi Johan, fancy meeting you here on the net.

I have e-mailed a short list of some recent articles I have in my collection
that relate to your query. Let me know if you don't receive them.

Give my regards to Barry, Alia, Linda, Jianli, etc, etc, etc.

See you around,

Mike Horsfall

Phone: (716)-275-8703
Fax: (716)-275-6007
E-mail: mjhf@troi.cc.rochester.edu
Newsgroups: bionet.molbio.methds-reagnts
From: toms@fcsparc6.ncifcrf.gov (Tom Schneider)
Subject: Re: Dispersion of Agarose
Organization: Frederick Cancer Research and Development Center
Distribution: bionet
Date: Sun, 10 Jan 1993 22:43:20 GMT

In article <01GTD30FXAKG0000FE@irri.cgnet.com> RSCOTT@IRRI.CGNET.COM writes:
>I can't help cursing myself whenever I prepare agarose gels and
>the suspension happens to overboil inside the microwave.

We had a pressure cooker attached to a timer.  Put the agaraose/buffer in, make
sure there is some water in the bottom, set the timer to 20 minutes, and go do
something else.  20 minutes later you have nice hot agar and it never boils
over!  No aggravation---but one does need to think ahead a bit.

  Tom Schneider
  National Cancer Institute
  Laboratory of Mathematical Biology
  Frederick, Maryland  21702-1201
  toms@ncifcrf.gov
From: bi__mvw@SELWAY.UMT.EDU (Michael van Waes)
Newsgroups: bionet.molbio.methds-reagnts
Subject: Need E.coli tRNA-Phe plasmid
Date: 7 Jan 93 18:24:37 GMT
Distribution: bionet


 Dear netters:

 I am in search of a plasmid that contains either the whole operon or just
 the sequence of tRNA-Phe from E.coli. Would anybody be so kind as to direct
 me to a good source, or be able to just send me some ??  :-).

 I would be eternally thankful, but for now I'll just say thanks for your
 help in advance.




           _________________________________________________________________
	   Michael van Waes	        |
	   Div. of Biol. Sci.	        |  "I never worry about the future, 
	   Univ. of Montana	        |       ... it comes soon enough. "
	   Phone: 406-243-5733  	| 
	   bi__mvw@selway.umt.edu	|            	Albert Einstein.
	   -----------------------------------------------------------------
From: RSCOTT@IRRI.CGNET.COM
Newsgroups: bionet.molbio.methds-reagnts
Subject: Dispersion of Agarose
Date: 10 Jan 93 11:08:49 GMT
Distribution: bionet

I can't help cursing myself whenever I prepare agarose gels and
the suspension happens to overboil inside the microwave. At
certain equally irksome times (especially with bigger preparations)
the gel remained undispersed while considerable volume has already
been lost during heating. I always cover the boiling flask
with a beaker but this didn't do so much to prevent excessive 
evaporation. I discovered a trick (perhaps I just rediscovered it on
my own) to avoid these pitfalls. I tried presoaking the gel overnight 
at the eve of my gel run. Wholla! The agarose dispersed quicker in the 
buffer. Dispersion took only about half the normal boiling time. By the 
time vigorous boiling commences the agarose particles were substantially
dissolved. Volume loss is therefore minimized. Presoaking allows 
the gel to swell and be hydrated. Alternatively, one can equilibrate
the gel suspension in a 55-650C bath (ca. 30 min) prior to boiling.
The same result is achieved. I think it's not much trouble having to
prepare the suspension well in advance and stand it for a while
especially if the gel consistency can be maintained for different
preparations of equal concentration.

R. P. Scott
Pathology Division
IRRI
Manila, Philippines
E-Mail: rscott@irri.cgnet.com
From: ccy@po.CWRU.Edu (Cheung C. Yue)
Newsgroups: bionet.molbio.methds-reagnts
Subject: Re: Glycogen for DNA Precipitation
Date: 8 Jan 93 22:21:03 GMT
Reply-To: ccy@po.CWRU.Edu (Cheung C. Yue)
Organization: Case Western Reserve University, Cleveland, OH (USA)
NNTP-Posting-Host: slc12.ins.cwru.edu


In a previous article, BIOSEE@UKCC.UKY.EDU (Stephanie Edelmann) says:


>We also must be using the Glycogen from Boehringer Mannheim.  Add 1ml to
                                                                    ^^
Surely you must mean 1 microliter!!  Otherwise it would have cost you
$50 or more per precipitation!  :-)

ccy@po.cwru.edu
-- 
Newsgroups: bionet.molbio.methds-reagnts
From: zsha@iastate.edu (Zhengyu Sha)
Subject: Re: western blotting IEF gels
Organization: Iowa State University, Ames IA
Distribution: bionet
Date: Mon, 11 Jan 1993 21:25:36 GMT

In <Pine.3.05.9301111440.A25760-a100000@post.its.mcw.edu> fgarbrec@POST.ITS.MCW.EDU (GARBRECHT) writes:

>I am interested in blotting protein mixtures onto nitrocellulose
>which have been separated by IEF.  The problem is that, as the proteins
>come to their isoelectric point in the IEF gel, they have a net zero
>charge and therefore won't migrate onto the nitrocellulose in the
>electroblotting step.  It occurs to be that there might be a couple
>of solutions: 1) to incubate the IEF gel in SDS containing buffer to
>allow the proteins to get a nice negatively charged coating of SDS
>or 2) to do capillary blotting instead of electroblotting (which I
>don't want to do.

>Has anybody tried this or have any suggestions?  Thanks alot

>Fred Garbrecht
>Medical College of Wisconsin
>fgarbrec@post.its.mcw.edu

Try the 2-D gel protocol and let the protein run just into the SDS gel. Then follow the normal protocol. We have western blotted the 2-D gel without any problems.
Newsgroups: bionet.molbio.methds-reagnts
Subject: Re: Need E.coli tRNA-Phe plasmid
From: robison1@husc10.harvard.edu (Keith Robison)
Date: 7 Jan 93 21:11:27 GMT
Distribution: bionet

bi__mvw@SELWAY.UMT.EDU (Michael van Waes) writes:


> Dear netters:

> I am in search of a plasmid that contains either the whole operon or just
> the sequence of tRNA-Phe from E.coli. Would anybody be so kind as to direct
> me to a good source, or be able to just send me some ??  :-

The gopher hole at gopher.nih.gov has a GenBank searching option which
can use AND.  Using this, I found:
M24882


which all have entries for E.coli tRNA-Phe

Keith Robison
Harvard University
Department of Cellular & Developmental Biology
Department of Genetics / HHMI

robison@biosun.harvard.edu 



ECOTRF
ECOTRFA
From: ez005587@chip.ucdavis.edu (David J. Meyer)
Newsgroups: bionet.molbio.methds-reagnts
Subject: Re: Glycogen for DNA Precipitation
Date: 10 Jan 93 05:31:57 GMT
X-Newsreader: Tin 1.1 PL3









In discussion RE: glycogen, Stephanie writes:

: We also must be using the Glycogen from Boehringer Mannheim.  Add 1ml to
: the DNA in ethanol and spin.  Glycogen is supposed to be a volume excluder
: (as far as I know), but we in our lab are convinced, that its main
: effect is a psychological one.  After adding glycogen, you all a sudden
: see a pellet.  That might be mostly glycogen, but it's a PELLET as oppose
: to nothing visible.  We haven't noticed any negative effects, yet. I've
: used it on DNA that was supposed to be cut, ligated, electrophoresed and
: probably others.
:  
: Stephanie

I often use glycogen to ppt. small amounts of/dilute DNA, and it works well.
However, at the concentrations used it probably is not functioning as a
volume excluder but as a coprecipitant; note that Stephanie correctly ob-
serves that the inclusion of glycogen results in a visible pellet which
is probably mostly glycogen.

It might be worth a separate posting, but while I have found that glycogen
interferes with almost no standard molecular manipulation I suspect that 
in high concentrations it may interfere with PCR using Taq polymerase.
Does anyone else have a similar suspicion? Evidence would, of course, be
appreciated.

David J. Meyer
djmeyer@ucdavis.edu
From: sbhattac@crc.ac.uk (Dr. S. Bhattacharya)
Newsgroups: bionet.molbio.methds-reagnts
Subject: DNA purification
Date: 5 Jan 93 13:21:34 GMT
Distribution: bionet
Original-To: methods@uk.ac.daresbury

>Geneclean II glassmilk efficiently removes DNA 200 bases or longer from
>agarose gels. Who has tried to isolate shorter (50-200) fragments.


>The MERMAID kit is designed for isolating shorter pieces of DNA (it is also
>a BIO101 product). I have never used it for isolating DNA directly from
>a gel, but I got essentially 100% recovery of a 20mer when precipitating
>from solution. It is designed for isolating from gels as well.

A very quick way to recover DNA from agarose gels is to spin 
it through siliconised glass wool in a mini Eppendorf with a small
hole at the bottom, suspend the min-eppendorf in a larger one
and spin at 6500 rpm on a bench top centrifuge, and collect the aqueous phase.
The DNA can be estimated by UV fluorescence (as it is still bound to EtBr). 
It can be further concentrated on a Amicon micro-column if required.
I have stopped using all other methods of DNA recovery. "If you can see it
you can clone it".
Shoumo
From: wetsel_r@wums.wustl.edu
Newsgroups: bionet.molbio.methds-reagnts
Subject: RE: Glycogen for DNA Precipitation
Date: 8 JAN 93 16:05:14 GMT
Organization: MIT PLASMA FUSION CENTER
NNTP-Posting-Host: msnews.wustl.edu

In a previous article, ashok@biochemistry.cwru.edu (Ashok Aiyar) wrote:
>In article <9301072225.AA18226@net.bio.net> KNECHT@UCONNVM.UCONN.EDU (Dave Knecht) writes:
> 
>>I remember reading somewhere of people using glycogen as a carrier for precipit
>>ation of DNA.  Can someone enlighten me as to a concentration to use, purity re
>>quired and any pluses or minuses of this procedure that you have encountered? J
>>Dave
> 
>I feel awful about saying this, but I just buy this stuff called Molecular 
>Biology Grade glycogen from Boehringer Mannheim.  I don't know the 
>concentration, but add 1 ul of it to precipitations of any volume.  It works, 
>and hasn't negatively interfered in any noticible may with any subsequent 
>steps such as restriction digestion, ligation, or in vitro transcription with 
>T7 RNA pol.
> 
>Ashok
----
We use it also and again, we buy it from BMB.  The stuff we get is 20mgs/ml 
and we often use 10 ug when precipitating PCR fragments in volumes much 
over 500 ul and especially when preciping trough eluted fragments.  Without 
looking it up (anyone feel free to correct...) I think the yeild of DNA in 
a precip starts to fall off when total is much below 50-100 ng/ml.  The 
addition of glycogen dramatically helps the yeild in DNA pptns when initial 
concentration is low...

Best regards,

===========================================================================
+  David L. Haviland, Ph.D.	     Internet:"haviland@kids.wustl.edu"   +
+  Washington Univ. School of Med.   A.K.A : The Compiler                 +
+  Dept. of Peds./Pulm. Box 8116     ICBM-Net : Just hit St. Louis        +
+  400 S. Kingshighway               &-6  <- User is Brain Dead...        +
+  St. Louis, MO  63110              FAX: 314-454-2476                    +
+  (314) 454-6076                                                         +
+          "Sometimes you have to stop and smell the pizza"               +
===========================================================================

Newsgroups: bionet.molbio.methds-reagnts
From: obrien@molbio.cbs.umn.edu (Darin O'Brien)
Subject: Re: Glycogen for DNA Precipitation
Organization: University of Minnesota
X-Newsreader: Tin 1.1 PL5
Distribution: bionet
Date: Sat, 9 Jan 1993 21:55:39 GMT

I routinely use 1/50 volume of 10mg/ml glycogen for precipitation of nucleic
acids.  I make up a stock in sterile water and just use the cheap stuff 
from Sigma (cat#G-8751).  I have heard of people extracting the stuff before
using it but I haven't found the need so I don't.  I precipitate on ice for 
10 minutes before centrifuging.  Best of luck.

darin
(obrien@molbio.cbs.umn.edu)

Newsgroups: bionet.molbio.methds-reagnts
From: danj@welchgate.welch.jhu.edu (Dan Jacobson)
Subject: Re: email/fax no. of HenrikGaroff/PeterLiljestorm of Karolinska
Organization: Johns Hopkins Univ. Welch Medical Library
Distribution: bionet
Date: Wed, 6 Jan 1993 14:44:57 GMT

In article <9301060620.AA22150@pelican.dbe.csiro.au> vrati@dbe.csiro.au (Sudhanshu Vrati) writes:
>
>I need the email address and fax no. of Prof. Henrik Garoff and Dr. Peter
>Liljestrom who are at the Department of Molecular Biology, Huddinge Hospital,
>Karolinska Institute, Stockholm, Sweden.  Any body out there who may help ?
>
>Thanks in anticipation,
>
>Sudhanshu
>

These would seem to be good sites for Liljestrom:

1) peli@thon.csb.ki.se

        thon.csb.ki.se
        Login name: peli                        In real life: Peter Liljestrom
        Directory: /home/cbt/peli               Shell: /bin/tcsh
        Last login Mon Dec 14 13:14 on ttyp2 from eel
        New mail received Wed Jan  6 00:05:40 1993;
          unread since Tue Jan  5 09:02:21 1993
        No Plan.

2) peli@eel.csb.ki.se (which refuses finger queries).


Best of luck,

Dan Jacobson

danj@welchgate.welch.jhu.edu

(BTW - this type of question is better suited for the 
bionet.users.addresses/bionaut newsgroup/mailing list.)
From: bl275@cleveland.Freenet.Edu (Dan Diaz)
Newsgroups: bionet.molbio.methds-reagnts
Subject: Re: what do you need to publish?
Date: 9 Jan 1993 10:39:58 GMT
Organization: Case Western Reserve University, Cleveland, OH (USA)
Reply-To: bl275@cleveland.Freenet.Edu (Dan Diaz)
NNTP-Posting-Host: hela.ins.cwru.edu


In a previous article, naffara@crc.ac.uk (Dr. N.A. Affara) says:

>This may seem a strange question to ask...
>
>If I have the sequence of a new gene and have mapped it to a specific 
>chromosome, is there anything else I need or is that enough for a paper?
>
>(Note I am not Dr Affara and I don't work at the MRC or whatever it says in the
>heading, I am a technician who doesn't agree with his boss...)

Sounds like a communication appropriate for Nucleic Acids Research, but if
the boss you dont agree with had anything to do with this work, you may
want to make peace with him/her before submitting anything.
-- 
Dizzy Dan         ddiaz@cwru.bitnet      bl275@cleveland.freenet.edu
Department of Molecular Genetics - Albert Einstein College of Medicine
"My opinions are my own, and they should be yours as well"
From: masure@ROCKVAX.ROCKEFELLER.EDU
Newsgroups: bionet.molbio.methds-reagnts
Subject: (none)
Date: 9 Jan 93 04:33:44 GMT
Distribution: bionet

help
From: hardy@mighty.facs.fccc.edu (Randy Hardy)
Newsgroups: bionet.molbio.methds-reagnts
Subject: Subtraction and PCR?
Date: 4 Jan 93 22:25:04 GMT
Organization: I.C.R., Fox Chase Cancer Center

A question for PCR experts: can one generate subtracted libraries using RT-PCR?
 Our problem is that the starting amounts of message are very tiny (ie, from
rare normal cells) so that traditional RNA subtraction is not feasible.  Seems
like I've heard some discussion before on using anchored PCR to generate large
amounts of material for subtraction, just wondered whether anyone has any
experience and/or recommendations.  Thanks in advance...

R. Hardy <hardy@mighty.facs.fccc.edu>
I.C.R., Fox Chase Cancer Center, 7701 Burholme Ave., Philadelphia, PA 19111,
USA
voice 215-728-2463   fax   215-728-2412
From: RSCOTT@IRRI.CGNET.COM
Newsgroups: bionet.molbio.methds-reagnts
Subject: Re: Dispersion of Agarose
Date: 12 Jan 93 00:11:34 GMT
Distribution: bionet

In article <01GTD30FXAKG0000FE@irri.cgnet.com>, RSCOTT@IRRI.CGNET.COM writes:
> I can't help cursing myself whenever I prepare agarose gels and
> the suspension happens to overboil inside the microwave. At
> certain equally irksome times (especially with bigger preparations)
> the gel remained undispersed while considerable volume has already
> been lost during heating. I always cover the boiling flask
> with a beaker but this didn't do so much to prevent excessive
> evaporation. I discovered a trick (perhaps I just rediscovered it on
> my own) to avoid these pitfalls. I tried presoaking the gel overnight
> at the eve of my gel run. Wholla! The agarose dispersed quicker in the
> buffer. Dispersion took only about half the normal boiling time. By the
> time vigorous boiling commences the agarose particles were substantially
> dissolved. Volume loss is therefore minimized. Presoaking allows
> the gel to swell and be hydrated. Alternatively, one can equilibrate
> the gel suspension in a 55-650C bath (ca. 30 min) prior to boiling.
> The same result is achieved. I think it's not much trouble having to
> prepare the suspension well in advance and stand it for a while
> especially if the gel consistency can be maintained for different
> preparations of equal concentration.
>
> R. P. Scott

I apologize for not mentioning that I do the overnight presoaking in the
ref in order to avoid microbial growth. Thanks Andrew Cockburn for raising
the issue of microbial contamination.

R.P. Scott
IRRI
E-mail: rscott@irri.cgnet.com
From: KNECHT@UCONNVM.UCONN.EDU (Dave Knecht)
Newsgroups: bionet.molbio.methds-reagnts
Subject: Glycogen for DNA Precipitation
Date: 7 Jan 93 22:23:09 GMT
Distribution: bionet

I remember reading somewhere of people using glycogen as a carrier for precipit
ation of DNA.  Can someone enlighten me as to a concentration to use, purity re
quired and any pluses or minuses of this procedure that you have encountered? J
Dave