HDTenEightyP/NetNews huggingface dataset:
From: TAFFETS@SNYSYRV1.bitnet
Newsgroups: bionet.immunology
Subject: Adjuvants
Date: 11 Oct 92 23:57:00 GMT
Distribution: bionet



     I'm wondering if anyone has any opinions on the new adjuvants that
have appeared in the past few years.

     In the past I have been successful at making good antisera using
complete Freund's adjuvant with my antigen.  In the last year I've tried
several of the new commercially available adjuvants (at the request of the
animal use committee).  I obtained antisera with a high titre by ELISA but
these sera were ineffective in some assays especially western blotting.
While it might be the antigens that I've used, I keep wondering about the
adjuvants.  I've noticed that every report describing these adjuvants only
has ELISA data and does not test the resulting serum in a variety of
assays.  Is it possible that these adjuvants induce a high titered serum
with low affinity or avidity?  Has anyone had great success (or failure)
with these adjuvants?  Are any of these adjuvants better than others?

     Thanks for your input.

        Steve
*******************************************************************************
Steven Taffet, Ph.D.
Associate Professor
Department of Microbiology and Immunology
S.U.N.Y. Health Science Center
750 E. Adams Avenue
Syracuse, New York 13210

Phone (315) 464-5419
FAX   (315) 464-4417
Bitnet  TAFFETS@snysyrv1
Internet TAFFETS@vax.cs.hscsyr.edu
*******************************************************************************

Newsgroups: bionet.immunology
From: horton@molbio.cbs.umn.edu (Robert Horton)
Subject: Re: Generating somatic diversity
Organization: University of Minnesota
X-Newsreader: Tin 1.1 PL5
Date: Thu, 15 Oct 1992 16:53:37 GMT

	In chicken immunoglobulin light chains (I think lambda) diversity
is generated by a "gene conversion-like" mechanism, in which sequence is
copied from "pseudogenes" into the one properly rearranged V region gene.
I don't know if this mechanism is used in other critters for Ig, but
it is intriguingly similar to the process of MHC diversification in both
humans and mice, though that takes place on an evolutionary scale.

--
Bob Horton            /\ "Crash programs fail because of the theory that
U. of Minnesota, CBS  || with nine women pregnant you get a baby a month" 
1479 Gortner Ave.    /||\   -Werner von Braun.  Disclaimer:"Bob who?"
St. Paul, MN 55108    ^^   horton@molbio.cbs.umn.edu/(612) 624-3790
Newsgroups: bionet.immunology
From: ULIMJ@CSPUNI12.EARN (Milan Jira)
Subject: Food Allergy...
Original-To: immuno@UK.AC.DARESBURY
Distribution: bionet
Date: Tue, 13 Oct 1992 21:18:29 GMT

    As I remember the issue of foof allergy problems have been already
discussed in this USENET/INTERNET/Interest group.
 
     Friend of my, memmber of the 3rd Medical faculty, Charles Univers.,
Prague, Czechoslovakia IS WILLIng to investigate this problem in a grant
project. The clinical increase of kids allergies stress her to make this appeal
 and willing to apply.
 
 Specific questions are:
 
   1. Is anybody on the list who would cooperate or participate ???
 
   2. Does anybody some clinical and addtional problems in childhood practice?
 
   3. Can anybody recommend IN VITRIO (reliable) non-isotopic (ELISA or strip)
      laboratory method he is succesfully using and give the reference OR !!!
      (from Czechoslovakia we still are unable to dial 800-XXXXX) to mention
      toll free our tremendeous interest to be faxed on +42-2-741075 or e-maile
d on the address below.
   4. An option to m{_g#ake a join project would certainly not excluded and mig
ht be discussed privatedly on e-mail.
   5. It is expected that due to the importance of this problems most of the
      cost arised from this projecxt could be covered from Czech Ministry of He
alth or affiliated insititions.
 
      Looking forward to your replies,
 
 
  Milan Jira, M.D., Ph.D., Head, Dept. of Clinical Immunology,
     Charles Univers., Faculty of Medicine, Prague, Czechoslovakia
BITNET:ULIMJ@CSEARN.BITNET         Internet: ulimj@aci.cvut.cs    s;nyM
Newsgroups: bionet.immunology
From: scott@sparc.brc.ubc.ca (Scott Pownall)
Subject: Re: What happened at Budapest?
Keywords: Somatic Hypermutation in Budapest
Organization: B. R. Centre Ltd.
Distribution: brc
Date: Sat, 10 Oct 92 05:25:39 GMT


	I am interested in the presentations concerning somatic mutation given at the Congress. Did anyone go to those particular sessions?
	What were the details of results of Giusti and Manser`s work in which they created transgenic mice carrying various VDJ segments. What are the indispencsable regions 3' of the intronic IgH enhancer?
	Ditto for the work of Weber, Berry and Latham Clafin using a kappa ig transgene with an enlarged leader intron.
	Ursula Storb's abstract says the targeting sequences are being defined. How? And what is the story on Ssm-1, the methylation control gene, with respect to SM.
	Any other congressional (or non-congressional!) info regarding SM would be of interest.


Scott Pownall,  {Dr wannabe}
Email:		scott@sparc.brc.ubc.ca
Regmail:	Biomedical Research Centre,UBC,Vancouver,CANADA V6T 1Z3
Fax: 		(604) 822-7815
 

PS: Were they really that short?
From: your-email-addr@a1.mscf.upenn.edu (Jesse M. Goldman)
Newsgroups: bionet.immunology
Subject: Generating somatic diversity
Date: 14 Oct 92 18:57:29 GMT
Followup-To: bionet.immunology
Organization: University of Pennsylvania


We know that point mutations are introduced into the variable regions
of immunoglobin genes when rechallenged with antigen and b cells are
selected which have higher affinity than the "mother" B-cell.  I know this
probably happens in germinal centers and that b-cells producing either
self directed immunoglobin or ineffective antibodies are delected.
But does anyone have ideas on how genes produce rapid rates of mutation?
And especially involving only variable regions?
From: philstas@rc1.vub.ac.be (Stas Philippe)
Newsgroups: bionet.immunology
Subject: Re: Journal Watch: Antigen dose-response reevaluation
Summary: Commends on Budapest
Date: 6 Oct 92 13:46:01 GMT
Organization: Vrije Universiteit Brussel, Brussels


Morning all,

Some time ago people asked on new evolutions on the Immunological
Congress. Well, 
I've been only to the antigen presentation stuff.

The most interesting point is that people from McMichaels lab
said several times a class II crystal will be published very soon

They also are ready for a major brealthrough on the field of
the proteasomes.

Hope this helps

btw, does anyone has a (short) good overview of all different immunological
and other cells? (f.i. hematopoietic, granulocytic, neutrophilic, ...), 
together with their function?

Thanks

Philippe Stas,
philstas@vub.ac.be
Newsgroups: bionet.immunology
From: ULIMJ@CSEARN.EARN (Milan Jira)
Subject: Users registered at Daresbury..
Original-To: immuno@UK.AC.DARESBURY
Distribution: bionet
Date: Fri, 16 Oct 1992 12:56:09 GMT

    Facilities in Daresbury node do not permit the user to REVIEW (command
for Discussion List on BITNET) who is subscribing actually this list.
    It is posssible  automatically, or not?
 
    If not,  maybe some of the readers would appreciate (I DO) to know it.
 
        Best,
 
 Milan Jira, MD, PhD.
 Dept. Immunology, 3rd Medical Faculty,
 Charles University, Prague, Czechoslovakia
Newsgroups: bionet.immunology
From: kja@cbnewsf.cb.att.com (krista.j.anderson)
Subject: Pointer to cow's milk intolerance article
Organization: AT&T
Date: Fri, 16 Oct 1992 19:13:44 GMT

This pointer is to let you know I have posted a layperson-to-
layperson article on cow's milk in sci.med, just in case anyone
from bionet would like to check it for errors.
-- 
Krista Anderson, att!ihlpf!krista
From: rsorense@CC.GETTYSBURG.EDU (Ralph A. Sorensen)
Newsgroups: bionet.immunology
Subject: Duesberg and AIDS
Date: 13 Oct 92 12:14:38 GMT
Distribution: bionet

In the September 25 issue of Science, Peter Duesberg once again puts
forth his argument that HIV is a symptom, rather than a cause of AIDS.
Without belaboring the point, can someone simply state the reasons for
Duesberg's contraversial position? My immunology students ask about this
and I do poorly at identifying the crux of his argument. At its base, 
I guess, Duesberg contends that there is a different cause for this 
chronic cell-mediated immunodeficiency. What might that be? Is Duesberg a
lone ranger?

                              Ralph A. Sorensen                                
                              Biology Department, Gettysburg College             
                              (717) 337-6168
                              rsorense@cc.gettysburg.edu
From: philstas@rc1.vub.ac.be (Stas Philippe)
Newsgroups: bionet.immunology
Subject: Re: Some questions on AIDS
Summary: reply:re:re:re:re:some questions
Date: 6 Oct 92 13:50:39 GMT
Organization: Vrije Universiteit Brussel, Brussels

> 2) I have heard that the virus has a half life on a dry surface of 7 
> seconds.  It is very sensitive to drying, thus, doorknobs and toilet seats 
> are definately out of the question!

Could you please send a reference on this? I searched for the half life
time of the AIDS virus under several conditions, but did not find
one article on this subject.

 Hope this helps...


"If Man is five, Then the devil is six"
"And if the devil is six, Then GOD is seven"
"This monkey is going to heaven"
-------------------------------------------------------------
-  Philippe Stas                        philstas@vub.ac.be  -
-  Dept. of Cellular Immunology                             -
-  Free University of Brussels          tel: 32-2-359.03.58 -
-  Paardenstraat 65                     Fax: 32-2-359.03.90 -
-  B1640 St.Genesius Rode             Modem: 32-2-359.03.59 -
-  Belgium, Europe                           (2400,1,N,8,1) -
-------------------------------------------------------------
From: kristoff@net.bio.net (David Kristofferson)
Newsgroups: bionet.announce
Subject: WOMENBIO/bionet.women-in-bio begins operation!
Date: 16 Oct 92 00:14:32 GMT
Distribution: bionet
Organization: BIOSCI International Newsgroups for Biology


Cassandra Smith has posted her introductory message to the new
bionet.women-in-bio newsgroup.  I include the following information
for those who would like to participate:


on USENET:   bionet.women-in-bio


Mailing addresses *for postings only* (see below for subscriptions):

womenbio@net.bio.net 	  (in the Americas and Pacific Rim)

womenbio@daresbury.ac.uk  (in Europe, Africa, and Central Asia)


To request e-mail subscriptions if you don't have USENET access:

biosci@net.bio.net 	(in the Americas and Pacific Rim)

biosci@daresbury.ac.uk  (in Europe, Africa, and Central Asia)


PLEASE don't mail subscription requests to the newsgroup posting
addresses!!  You will just annoy other people on the list.

				Sincerely,

				Dave Kristofferson
				BIOSCI/bionet Manager

				kristoff@net.bio.net
From: kristoff@net.bio.net (David Kristofferson)
Newsgroups: bionet.announce
Subject: Re: New TurboGopher software
Date: 8 Oct 92 02:25:18 GMT
Organization: BIOSCI International Newsgroups for Biology

I picked up a copy of TurboGopher for the Mac this afternoon and was
genuinely impressed.  The response of this network client/server
program was *crisp* except for a few sites with sub-par Internet
connections.  If you have a Mac which can reach the Internet, this
program is *definitely* worth looking getting.  Gopher has been
described in the newsgroups on many occasions.  In case you have
somehow missed these messages, I will point out that not only does
Gopher provide a user-friendly way to FTP software/data, it also can
be used for text searching in ways similar to WAIS and IRX (it can in
fact use WAIS indexes).  It also contains access to local address
directories for a number of universities.  There are a number of nice
"gopher holes" in existence for biologists, especially the one
established by Don Gilbert at IUBIO in Indiana.

TurboGopher is definitely worth getting by FTP from Minnesota.

				Sincerely,

				Dave Kristofferson
				BIOSCI/bionet Manager

				kristoff@net.bio.net
From: cup@bones.biochem.ualberta.ca (Chris Upton)
Newsgroups: bionet.announce
Subject: Re: New TurboGopher software
Date: 8 Oct 92 17:15:50 GMT
Reply-To: cup@bones.biochem.ualberta.ca
Organization: University of Alberta

In article 13679@net.bio.net, kristoff@net.bio.net (David Kristofferson) writes:
>I picked up a copy of TurboGopher for the Mac this afternoon and was
>genuinely impressed.

I second that!!!! 
Besides all those fancy connections and "scientific jobs" it does,
if you poke your nose into your local gopher hole - there's some handy
chores it can probably perform. Here's a couple of examples:

Check out the latest computer prices at the University Bookstore
Find a campus phone number (when someone's run off with your directory)
Get you a local weather forecast
Find out how late the various libraries are open
Get info on the Canadian Constitutional Debate


---
Chris_Upton@darwin.biochem.ualberta.ca
Biochemistry Department
University of Alberta
Edmonton, AB T6H 2H7
Canada
From: kristoff@net.bio.net (David Kristofferson)
Newsgroups: bionet.announce
Subject: IMPORTANT!! - 1992 ANNUAL BIOSCI READERSHIP SURVEY - PLEASE RESPOND!!
Date: 16 Oct 92 23:12:53 GMT
Reply-To: biovote@net.bio.net

**********************************************************************
This is the *third* posting of this survey.  If you have already
responded, please do not respond again.
**********************************************************************

It's time to justify our existence once again by counting our
readership, so I'd ***GREATLY*** appreciate it if everyone who sees
this message respond to 2 or 3 simple questions (see SURVEY below).
Anyone who reads this message is requested to respond *regardless* of
their location or affiliation.  Only individual responses will be
counted, i.e. one e-mail/one person.  Duplicate votes will be
eliminated.  I will be reposting this message approximately weekly and
collecting responses up through 11/1/92.  Thanks in advance!!


------------------------------- SURVEY -------------------------------

(NOTE - You need not return this form.  Please simply reply with your
answers to the following questions.)

1) In what country are you located?

2) Did you see this message in your e-mail or by using some kind of
   newsreading software?

3) If you saw it using news software, what program do you use?

----------------------------------------------------------------------

Please use one of the addresses given below to send in your response
or respond to me directly at kristoff@net.bio.net.  This message is
also set with a Reply-To line to direct responses to the
biovote@net.bio.net address, but please use the Daresbury address
below (esp. in Europe) if you have any problems getting through to
biovote@net.bio.net.


Address to submit survey              Location        Network
------------------------              --------        -------
biovote@daresbury.ac.uk               U.K.            JANET
biovote@net.bio.net                   U.S.A.          Internet/BITNET


Thanks in advance for your time and help in sustaining BIOSCI!  The
results of this survey will be posted to BIONEWS/bionet.announce in
November.

				Sincerely,

				David Kristofferson, Ph.D.
				BIOSCI/bionet Manager

				kristoff@net.bio.net
From: kristoff@net.bio.net (David Kristofferson)
Newsgroups: bionet.announce
Subject: IMPORTANT!! - 1992 ANNUAL BIOSCI READERSHIP SURVEY - PLEASE RESPOND!!
Date: 8 Oct 92 00:46:43 GMT
Reply-To: biovote@net.bio.net

**********************************************************************
This is the second posting of this survey.  If you have already
responded, please do not respond a second time.
**********************************************************************

It's time to justify our existence once again by counting our
readership, so I'd ***GREATLY*** appreciate it if everyone who sees
this message respond to 2 or 3 simple questions (see SURVEY below).
Anyone who reads this message is requested to respond *regardless* of
their location or affiliation.  Only individual responses will be
counted, i.e. one e-mail/one person.  Duplicate votes will be
eliminated.  I will be reposting this message approximately weekly and
collecting responses up through 11/1/92.  Thanks in advance!!


------------------------------- SURVEY -------------------------------

(NOTE - You need not return this form.  Please simply reply with your
answers to the following questions.)

1) In what country are you located?

2) Did you see this message in your e-mail or by using some kind of
   newsreading software?

3) If you saw it using news software, what program do you use?

----------------------------------------------------------------------

Please use one of the addresses given below to send in your response
or respond to me directly at kristoff@net.bio.net.  This message is
also set with a Reply-To line to direct responses to the
biovote@net.bio.net address, but please use the Daresbury address
below (esp. in Europe) if you have any problems getting through to
biovote@net.bio.net.


Address to submit survey              Location        Network
------------------------              --------        -------
biovote@daresbury.ac.uk               U.K.            JANET
biovote@net.bio.net                   U.S.A.          Internet/BITNET


Thanks in advance for your time and help in sustaining BIOSCI!  The
results of this survey will be posted to BIONEWS/bionet.announce in
November.

				Sincerely,

				David Kristofferson, Ph.D.
				BIOSCI/bionet Manager

				kristoff@net.bio.net
From: kristoff@net.bio.net (Dave Kristofferson)
Newsgroups: bionet.announce
Subject: bionet.biochem.carotenoids does NOT PASS - 39 YES, 3 NO
Date: 16 Oct 92 07:22:27 GMT


The turnout for the vote on the CAROTENOIDS newsgroup was rather
small; it came nowhere close to obtaining 80 votes, so it will not be
created as a BIOSCI newsgroup.

There are two options available.  The newsgroup proposal can either be
reconsidered in three months or else the discussion leader, Dr.
Scolnik, can contact me about creating this group as a trial mailing
list for six months as I outlined in my posting to this forum
yesterday.

				Sincerely,

				Dave Kristofferson
				BIOSCI/bionet Manager

				kristoff@net.bio.net
From: kristoff@net.bio.net (Dave Kristofferson)
Newsgroups: bionet.announce
Subject: New TurboGopher software
Date: 7 Oct 92 22:29:40 GMT

This looks like an item of widespread interest to biologists using the
net.

-Dave K.

----------------------------------------------------------------------
From comp.sys.mac.announce Wed Oct  7 15:27:25 1992
From: kristoff@net.bio.net (Dave Kristofferson)
Newsgroups: bionet.announce
Subject: New BIOSCI Service - "proto-newsgroups" 8-)
Date: 16 Oct 92 01:21:55 GMT


I have always been torn between wanting to foster the growth of
electronic communications while not going overboard and creating
millions of newsgroups that nobody uses.  Now that GenBank is (almost)
off my shoulders (back, etc.), I am glad to announce a new service
from BIOSCI.

We will be happy to establish and administer a straight mailing list
*without* an associated USENET newsgroup for a six month trial period
for anyone that wants to try to form a new electronic community in the
biological sciences (I stress that the topics are limited to
professional communications though.).

The mailing lists will be maintained *initially* only at net.bio.net
instead of at both BIOSCI sites, and will not be added to the BIOSCI
information sheet listing.  It will be the responsibility of the
person who proposes the list to get it up and running within the six
month period.  They will have to handle promotion; our involvement at
BIOSCI at net.bio.net will be limited to creating the list, putting
out one announcement about it, and handling subscription requests.

After six months, the list will be put out for discussion and a vote
according to our usual procedures (unless the organizer throws in the
towel 8-).  If it passes it will becomne a full-fledged BIOSCI
newsgroup at both net.bio.net and daresbury.ac.uk and will also have a
parallel USENET newsgroup.  If it fails the mailing list will also be
shut down.

This seems like the best solution to help embryonic interest groups
get started if they have an enthusiastic organizer.  It minimizes our
technical investment, does not impinge on USENET, and provides time for
the budding "newsgroup" to either flourish or whither.

I should emphasize that this new service does not preclude people who
have an idea that has widespread appeal from following our current
newsgroup creation policy and going to a vote after a 10 day
discussion.

If you have an idea that you would like to try, feel free to send it
to biosci@net.bio.net.

				Sincerely,

				Dave Kristofferson
				BIOSCI/bionet Manager

				kristoff@net.bio.net
From: kristoff@net.bio.net (David Kristofferson)
Newsgroups: bionet.announce
Subject: CALL FOR DISCUSSION: bionet.virology
Date: 12 Oct 92 18:46:06 GMT
Distribution: bionet

We have received a proposal for another new newsgroup.

bionet.virology (e-mail address: VIROLOGY).

This group was proposed and voted on previously during the summer
months and fell short of approval by only 19 votes (61 YES, 2 NO).
Three months have passed and the discussion leader would like to try
again now.

Discussion will now be open through 22 October on
BIOFORUM/bionet.general (*not* on BIONEWS/bionet.announce).

Please do not post one-line messages saying only "I am in favor of
such a newsgroup".  We will collect votes later.  The discussion
period is an opportunity for anyone to bring up reservations about the
proposed charter and to propose modifications prior to the vote.  The
discussion leader may submit a revised proposal in light of the
discussion, and the new charter would be included in the call for votes
which will go out on October 23rd.

Please be aware that only one vote is counted per e-mail address, so
it is necessary to have your own account in order to vote.  Multiple
votes from the same address are not accepted.  If you are interested
in the following newsgroup, but do not have an e-mail address of your
own, please obtain one from your computer center before the call for
votes is issued.

----------------------------------------------------------------------
Proposal to establish bionet.virology

Discussion leader:   Robert Coelen
                     Department of Microbiology
                     The University of Western Australia

bionet.virology charter:

It is the intention to use bionet.virology as a group for:

(a) the posting of information relating to:

        (1) Virus taxonomy
        (2) Virus structure and morphogenesis
        (3) Multiplication of viruses
        (4) Virus genetics and evolution
        (5) Epidemiology and Pathogenesis of viruses
        (6) Host response to viral infections
        (7) Vaccinology and antiviral chemotherapy
        (8) Any other matters pertaining to viruses not 
            mentioned above

Whilst the group name is virology and the charter above 
only mentions viruses, postings in the above categories 
about infectious agents such as viroids and prions are to 
be included in this group. Discussion about evolution 
should be restricted to viral evolution only.
(b) the posting of news on methods or reagents relating 
to techniques used uniquely for or with viruses
It is not intended to include postings on methods or 
reagents common to molecular biology. These are better 
placed in bionet.molbio.methds-reagnts.
(c) the posting of articles from various 'non-journal' 
publications such as CDI weekly, and other infectious 
diseases bulletins and the various virus newsletters (eg 
Virus Information Exchange Newsletter)
(d) the posting of articles relating to current outbreaks 
of viral disease around the world
------------------------------------------------------------------