HDTenEightyP/NetNews huggingface dataset:
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human chromosome 4 sequence-tagged site STS4-447
Date: 21 Aug 92 10:10:32 GMT
Distribution: bionet
Checksum: 07273     3

LOCUS       HUM4STS447    366 bp ds-DNA             PRI       21-AUG-1992
DEFINITION  Human chromosome 4 sequence-tagged site STS4-447
ACCESSION   L00868
KEYWORDS    STS; human chromosome 4; sequence tagged site.
SOURCE      Homo sapiens, clone p4-455 from a chromosome 4 plasmid library;
            vector pBluescript II KS + (Stratagene)
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 366)
  AUTHORS   Goold,R.D., diSibio,G., Dugaiczyk,A., Smith,K.A., Xu,H.,
            Magrane,G., Lang,D.B., Cox,D.R., Masters,S.B. and Myers,R.M.
  TITLE     Sequence-Tagged Sites for Human Chromosome 4
  JOURNAL   Unpublished (1992) see COMMENT for author address
  STANDARD  full automatic
COMMENT     PCR components: 25 ng of human genomic DNA, 10 pmol of each
            oligonucleotide, 200 micro-M dNTPs, 0.25 U Taq  polymerase (Cetus)
            in 10 micro-l of 50 mM KCl-20 mM  Tris-HCl, pH 8.3 (at room temp),
            2.5 mM MgCl-2.  Initial denaturation at 94degC for 1.5 min, then 30
            cycles of  94degC for 15 sec, 62degC for 23 sec, and 72degC for 30
            sec, followed by a final extension at 72degC for 3.5 min, using a
            Perkin-Elmer 9600 thermocycler. PCR-amplified product size 222 bp.
            Sequence submitted by:
            Human Genome Mapping Center
            Box 0925
            University of California San Francisco
            San Francisco, CA 94143-0925  USA
            Phone: (415) 502-1612   Fax: (415) 476-8391
            e-mail: hgmcprobes@cgl.ucsf.edu
FEATURES             Location/Qualifiers
     misc_feature    7..30
                     /label=Primer1
     misc_feature    complement(206..228)
                     /label=Primer2
BASE COUNT       95 a     86 c     52 g    133 t
ORIGIN
        1 gatcctctca gcttcttcaa agagcagtaa ttttcagaat tgacctttaa tttattgcta
       61 ctttagttta cttattttta tcacaggtcc ttatggctgt tctcacagtt tcagccccaa
      121 cctacatgcg aaacactcct tgcccaactc gctggttctc gccattgggt tttacttgag
      181 agtttcttca tcaaaggagt ccagccttcc ctgcacctga cagaatctca atcttatatc
      241 ctcccaccat tttcctccat aacactagtt atgaagttaa attatacaat ttcatgggtt
      301 gtttaggatt tttttcctct tcaaaacttc taggaagata gaatatatct gtgtaactta
      361 aaattg
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Rickettsia sp. (clone PRB FISF 1), 5' end CDS.
Date: 21 Aug 92 10:12:47 GMT
Distribution: bionet
Checksum: 25175     2

LOCUS       RIRGENSEQ     491 bp ds-DNA             BCT       21-AUG-1992
DEFINITION  Rickettsia sp. (clone PRB FISF 1), 5' end CDS.
ACCESSION   M99391
KEYWORDS    .
SOURCE      Rickettsia sp. DNA.
  ORGANISM  Rickettsia sp.
            Prokaryotae; Gracilicutes; Scotobacteria;
            Rickettsias and Chlamydias; Rickettsiales; Rickettsiaceae.
REFERENCE   1  (bases 1 to 491)
  AUTHORS   Baird,R.W.
  TITLE     characterization and comparison of australian human spotted fever
            group rickettsial isolates
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     CDS             30..>491
                     /codon_start=1
                     /translation="MKLLSKIMIIALATSMLQACNGPGGMNKQGTGTLLGGAGGALLG
                     SQFGKGKGQLVGVGVGALLGAVLGGQIGAGMDEQDRRLAELTSQRALETAPSGSNVEW
                     RNPDNGSYGYVTPNKTYRNSTGQDCRVYTQTVVIGGKQQKAYGNACRQPDGQ"
BASE COUNT      157 a     87 c    122 g    125 t
ORIGIN
        1 taattatata ttaatttaga gagaattata tgaaactatt atctaaaatt atgattatag
       61 ctcttgcaac ttctatgtta caagcctgta acggtccggg cggtatgaat aaacaaggta
      121 caggaacact tcttggcggt gctggcggcg cattacttgg ttctcaattc ggtaagggca
      181 aaggacagct tgttggagta ggtgtaggtg cattacttgg agcagttctt ggtggacaaa
      241 tcggtgcagg tatggatgag caggatagaa gacttgcaga gcttacctca cagagagctt
      301 tagaaacagc tcctagtggt agtaacgtag aatggcgtaa tccggataac ggcagttacg
      361 gttacgtaac acctaataaa acctatagaa atagcactgg tcaagattgc cgtgtgtaca
      421 ctcaaacagt tgtaataggc ggaaaacaac aaaaagcata cggtaatgca tgccgccaac
      481 ctgacggaca a
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Lentinus strigosus ribosomal RNA (49LR6) large subunit, partial.
Date: 19 Aug 92 10:01:36 GMT
Distribution: bionet
Checksum: 62039     2

LOCUS       LENRRLSAJ     249 bp ss-rRNA            RNA       19-AUG-1992
DEFINITION  Lentinus strigosus ribosomal RNA (49LR6) large subunit, partial.
ACCESSION   M98634
KEYWORDS    large subunit ribosomal RNA.
SOURCE      Lentinus strigosus (library: Duke culture collection 631) cDNA to
            rRNA.
  ORGANISM  Lentinus strigosus
            Eukaryota; Fungi; Eumycota; Basidiomycotina; Hymenomycetes;
            Agaricales; Tricholomataceae.
REFERENCE   1  (bases 1 to 249)
  AUTHORS   Hibbett,D.S. and Vilgalys,R.
  TITLE     Phylogenetic relationships of the basidiomycete genus Lentinus
            inferred from molecular and morphological characters
  JOURNAL   Unpublished (1992)
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     rRNA            <1..>249
                     /note="Approximately aligns to region between positions
                     845 to 1093in Saccharomyces cerevisiae large subunit
                     nuclear rRNA."
                     /product="large subunit ribosomal RNA"
                     /citation=[1]
BASE COUNT       71 a     49 c     63 g     66 t
ORIGIN
        1 ggaaactctg gtggaggctg tagcgattct gacgtgcaaa tcgatcgtca aatttgggta
       61 taggggcgaa agactaatcg aaccatctag tagctggttc ctgccgaagt ttccctcagg
      121 atagcagaaa ctcatgtcag atttatgtgg taaagcgaat gattagaggc cttggggttg
      181 aaacaacctt aacctattct caaactttaa atatgtaaga acgagccgtc tcttaattgg
      241 accgctcgg
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human chromosome 4 sequence-tagged site STS4-157
Date: 21 Aug 92 10:07:06 GMT
Distribution: bionet
Checksum: 26265     3

LOCUS       HUM4STS157    427 bp ds-DNA             PRI       21-AUG-1992
DEFINITION  Human chromosome 4 sequence-tagged site STS4-157
ACCESSION   M95242
KEYWORDS    STS; human chromosome 4; sequence tagged site.
SOURCE      Homo sapiens, clone C4-93 from Los Alamos National Laboratory
            chromosome 4 cosmid library, plate 4-1-1R, pos 3D; vector SuperCos
            1 (Stratagene)
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 427)
  AUTHORS   Goold,R.D., diSibio,G., Dugaiczyk,A., Smith,K.A., Xu,H.,
            Magrane,G., Lang,D.B., Cox,D.R., Masters,S.B. and Myers,R.M.
  TITLE     Sequence-Tagged Sites for Human Chromosome 4
  JOURNAL   Unpublished (1992) see COMMENT for author address
  STANDARD  full automatic
COMMENT     PCR components: 25 ng of human genomic DNA, 10 pmol of each
            oligonucleotide, 200 micro-M dNTPs, 0.25 U Taq  polymerase (Cetus)
            in 10 micro-l of 50 mM KCl-20 mM  Tris-HCl, pH 8.3 (at room temp),
            2.5 mM MgCl-2.  Initial denaturation at 94degC for 1.5 min, then 30
            cycles of  94degC for 15 sec, 62degC for 23 sec, and 72degC for 30
            sec, followed by a final extension at 72degC for 3.5 min, using a
            Perkin-Elmer 9600 thermocycler. PCR-amplified product size 159 bp.
            Sequence submitted by:
            Human Genome Mapping Center
            Box 0925
            University of California San Francisco
            San Francisco, CA 94143-0925  USA
            Phone: (415) 502-1612   Fax: (415) 476-8391
            e-mail: hgmcprobes@cgl.ucsf.edu
FEATURES             Location/Qualifiers
     misc_feature    21..44
                     /label=Primer1
     misc_feature    complement(154..179)
                     /label=Primer2
BASE COUNT      109 a     91 c     80 g    140 t      7 others
ORIGIN
        1 ccctgncaac aattccttag gaataggtgt gtttatccca tcacagaaaa ggaaaacaaa
       61 attgacagan gtcaggnaat tttcccatgg tctcacagca gatgtgtgac agangtcagg
      121 aaattttccc atggtctcac agcagatgtg ttacagaacc agtatctttt tgttttgttt
      181 tgttttaaat tattatattt taagttctgg ggtacatgtg cagaatgtgc aggtttgtta
      241 cataagtata cacgtgctat ggtggtttgc tgcacccatc aacccatnat ntacattagt
      301 tgtttctttt aatgctattc ctcgcctagc tctccacctn ccgacaggcc ccggtgtgtg
      361 atgttcccct ccttgtgtcc acgtcagaac cagtatctta atatgatcta tcttagcata
      421 aaatcct
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Pan troglodytes MHC DQB1*02 gene, partial cds, clone ChDQB102.
Date: 12 Aug 92 10:00:33 GMT
Distribution: bionet
Checksum: 52548     2

LOCUS       CHPMHDQBD     246 bp ds-DNA             PRI       12-AUG-1992
DEFINITION  Pan troglodytes MHC DQB1*02 gene, partial cds, clone ChDQB102.
ACCESSION   M81262
KEYWORDS    major histocompatibility complex.
SOURCE      Pan troglodytes (individual_isolate Ingrid) blood DNA.
  ORGANISM  Pan troglodytes
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Pongidae.
REFERENCE   1  (bases 1 to 246)
  AUTHORS   Bontrop,R.E., Jonker,M., van Weeren,P., Kenter,M. and Otting,N.
  TITLE     MHC-DQB repertoire variation in hominoid and old world primate
            species
  JOURNAL   J. Immunol. 149, 461-470 (1992)
  STANDARD  full staff_entry
FEATURES             Location/Qualifiers
     CDS             <1..>246
                     /gene="MHC DQ-beta 1"
                     /label=exon 2
                     /codon_start=1
                     /translation="FQFKGMCYFTNGTERVRLVTRYIYNREEFVRFYSDVGVYRAVTP
                     QGRPVAEYWNSQKEVLERTRAELDTVCRHNYEVAYRGI"
BASE COUNT       53 a     63 c     87 g     43 t
ORIGIN
        1 ttccagttta agggcatgtg ctacttcacc aacgggacgg agcgcgtgcg tcttgtaacc
       61 agatacatct ataaccgaga ggagttcgtg cgcttctaca gcgacgtggg ggtgtaccgc
      121 gcggtgacgc cgcaggggcg gcctgtcgcc gagtactgga acagccagaa ggaagtcctg
      181 gagaggaccc gggcggagtt ggacacggtg tgcagacaca actacgaggt ggcgtaccgc
      241 gggatc
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Brugia malayi Ras homolog of the TC4/Ran/Spil family, mRNA.
Date: 18 Aug 92 10:00:27 GMT
Distribution: bionet
Checksum: 35178     2

LOCUS       BRPRAS        905 bp ss-mRNA            INV       18-AUG-1992
DEFINITION  Brugia malayi Ras homolog of the TC4/Ran/Spil family, mRNA.
ACCESSION   M98810
KEYWORDS    .
SOURCE      Brugia malayi male adult cDNA to mRNA.
  ORGANISM  Brugia malayi
            Eukaryota; Animalia; Eumetazoa; Nematoda; Secernentea; Spiruria;
            Spirurida; Spirurina; Filarioidea; Gnathostomatidae.
REFERENCE   1  (bases 1 to 905)
  AUTHORS   Dissanayake,S., Min,X. and Piessens,W.F.
  TITLE     Filarial parasites contain a Ras homolog of the TC4/Ran/Spi1 Family
  JOURNAL   Unpublished (1992)
  STANDARD  full staff_entry
BASE COUNT      278 a    152 c    200 g    275 t
ORIGIN
        1 ggaattcggg cagcataagt aatggcaact ggtgatgata tcccgacgtt taaacttgtg
       61 cttgttggtg atggcggtac aggcaagacg acatttgtca aaaggcatct tacaggagat
      121 tttgagaaga aatacgttgc aactttgggt gtggaagttc atccactgat atttcacaca
      181 aacagaggtc agattcgatt taatgtttgg gatacagctg gacaagagaa atttggcgga
      241 ttgcgagatg gttattacat acaaggtcag tgcgcattta atatgttcga tgtaacagca
      301 cgtgttactt acaaaaacgt tccaaactgg catcgtgatt tagtgcgcgt gtgtgaaaat
      361 atcccgatag ttttatgtgg gaataaggta gatgtgaaag acagaaaagt caaagctaag
      421 acgatcacat tccatcgaaa gaaaaatctg cagtactacg atatctcagc gaagagcaat
      481 tataatttcg aaaaaccttc cctatggctg gcccgaaaat tgttaggcga tccaaatcta
      541 gaatttgttg caatgcctgc acttgctcct cctgaagtgc agatggatcc aacaatggtt
      601 gcacagtatg aacaagaaat tgctgctgct gcgaacgctg aacttccaga cgatgatgag
      661 gatctttaat cgattgactg tttggaaagg ttttggcaca aaaaccaaga gttttttggg
      721 aagcattatt atcatattac tgttgttgcc gtgtttttta tgccatttat tgaaagattg
      781 ttttgtgttt gtttatcctg attttcgttc caaaaattta cataaatttg ggtatgtaag
      841 caaacaaaat ttttcattta tacattgaac atctaccgtg atcgctatat caaattcacg
      901 aattc
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human chromosome 4 sequence-tagged site STS4-198
Date: 21 Aug 92 10:07:39 GMT
Distribution: bionet
Checksum: 36361     3

LOCUS       HUM4STS198    424 bp ds-DNA             PRI       21-AUG-1992
DEFINITION  Human chromosome 4 sequence-tagged site STS4-198
ACCESSION   L00902
KEYWORDS    STS; human chromosome 4; sequence tagged site.
SOURCE      Homo sapiens, clone C4-123 from Los Alamos National Laboratory
            chromosome 4 cosmid library, 1F9; vector SuperCos 1 (Stratagene)
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 424)
  AUTHORS   Goold,R.D., diSibio,G., Dugaiczyk,A., Smith,K.A., Xu,H.,
            Magrane,G., Lang,D.B., Cox,D.R., Masters,S.B. and Myers,R.M.
  TITLE     Sequence-Tagged Sites for Human Chromosome 4
  JOURNAL   Unpublished (1992) see COMMENT for author address
  STANDARD  full automatic
COMMENT     PCR components: 25 ng of human genomic DNA, 10 pmol of each
            oligonucleotide, 200 micro-M dNTPs, 0.25 U Taq  polymerase (Cetus)
            in 10 micro-l of 50 mM KCl-20 mM  Tris-HCl, pH 8.3 (at room temp),
            2.5 mM MgCl-2.  Initial denaturation at 94degC for 1.5 min, then 30
            cycles of  94degC for 15 sec, 62degC for 23 sec, and 72degC for 30
            sec, followed by a final extension at 72degC for 3.5 min, using a
            Perkin-Elmer 9600 thermocycler. PCR-amplified product size 209 bp.
            Sequence submitted by:
            Human Genome Mapping Center
            Box 0925
            University of California San Francisco
            San Francisco, CA 94143-0925  USA
            Phone: (415) 502-1612   Fax: (415) 476-8391
            e-mail: hgmcprobes@cgl.ucsf.edu
FEATURES             Location/Qualifiers
     misc_feature    51..75
                     /label=Primer1
     misc_feature    complement(235..259)
                     /label=Primer2
BASE COUNT       98 a     78 c     58 g    186 t      4 others
ORIGIN
        1 anggttttaa tttgcatttc tctaatggtt acttatgttt agcatttttn catatacttc
       61 ttggccagtt gtatgtcttc ttttagagat gtctattcag ctcatttgct ccttttttaa
      121 ttagattctn cttttaactg ttgaattgtt tgagttcctt atatattctg gacattcatc
      181 tcttgtcaga taaatagttt tcaaatactc tctcccattt tgccagattc ctcttcattc
      241 tgttgattgt ttcctgaaca atacagaagc ttttaagatt gatataatcc catttgtcta
      301 tatttgcctt tcttgcttat gcctttaagg tcttctccat aaaaccttta cccagaaaaa
      361 tgtactgaaa catttatggt tttgtccacc agtttcatag tgttgggtgn tacattttaa
      421 gtcc
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human immunodeficiency virus type 2 (FOENVA6) envelope protein
Date: 18 Aug 92 10:01:34 GMT
Distribution: bionet
Checksum: 57594     2

LOCUS       HIVENVA6      453 bp ss-RNA             VRL       18-AUG-1992
DEFINITION  Human immunodeficiency virus type 2 (FOENVA6) envelope protein
            fragment.
ACCESSION   M87073
KEYWORDS    envelope protein.
SOURCE      Human immunodeficiency virus type 2 uncultured blood mononuclear
            cells (11/89 sample) RNA.
  ORGANISM  Human immunodeficiency virus type 2
            Viridae; ss-RNA enveloped viruses; Positive strand RNA virus;
            Retroviridae; Lentivirinae.
REFERENCE   1  (bases 1 to 453)
  AUTHORS   Gao,F., Yue,L., White,A.T., Pappas,P.G., Barchue,J., Hanson,A.P.,
            Greene,B.M., Sharp,P.M., Shaw,G.M. and Hahn,B.H.
  TITLE     Human infection by genetically-diverse SIVsm-related HIV-2 in West
            Africa
  JOURNAL   Nature (1992) In press
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     misc_feature    1..453
                     /note="PCR derived HIV-2 envelope fragment"
                     /gene="env"
                     /note="putative"
                     /citation=[1]
BASE COUNT      185 a     77 c     81 g    110 t
ORIGIN
        1 aacgtggtca aaagacaaca aaaattgttg cgactaaccg tctgggagac taaaaacctc
       61 cagactaaag tcactgctat caaaaagtac ctaaagaacc aggcacagct aaattcatag
      121 gaatgcgcgt tcagacaggt ctgccacacc actgtaccat ggccaaataa tagtctcaca
      181 cctaactgaa acaatataac ttggcagaag tagaaaagaa aggttaattt cctaaaggca
      241 aatataacac agatgttaaa gaaagcacaa attcaacagg agaaaaacat gtataagtta
      301 caaaaactaa ataactggga tatatttggc aactggtttg accttacttc ttggattaag
      361 tatatacagt atggagtctt catagtagta ggtataatac tgttaagaat aataatctat
      421 ataatacaaa tgttaactag gttaagtaag gga
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Cucumber Mosaic Virus coat protein (CP) RNA, complete cds.
Date: 13 Aug 92 10:00:40 GMT
Distribution: bionet
Checksum: 16613     3

LOCUS       MCVCPAB       657 bp ss-RNA             VRL       13-AUG-1992
DEFINITION  Cucumber Mosaic Virus coat protein (CP) RNA, complete cds.
ACCESSION   M98500
KEYWORDS    coat protein.
SOURCE      Cucumber mosaic virus (individual_isolate PR36) cDNA to genomic
            RNA.
  ORGANISM  Cucumber mosaic virus
            Viridae; ss-RNA nonenveloped viruses; Isometric ss-RNA viruses;
            Cucumovirus.
REFERENCE   1  (bases 1 to 657)
  AUTHORS   Pappu,S.S., Pappu,H.R., Niblett,C.L. and Bird,J.
  TITLE     The nucleotide and the deduced amino acid sequences of coat protein
            genes of three Puerto Rican isolates of cucumber mosaic virus
  JOURNAL   Unpublished (1992)
  STANDARD  simple staff_entry
FEATURES             Location/Qualifiers
     CDS             1..657
                     /product="coat protein"
                     /gene="CP"
                     /codon_start=1
                     /translation="MDKSESTSAGRNRRRRPRRGSRSASSSADANFRVLSQQLSRLNK
                     TLAAGRPTINHPTFVGSERWKPGYTFTSITLKPPKIDRGSYYGKRLLLPDSVTEYDKK
                     LVSRIQIRVNPLPKFDSTVWVTVRKVPASSDLSVAAISAMFADGASPVLVYQYAASGV
                     QANNKLLYDLSAMRADIGDMRKYAVLVYSKDDALETDELVLHVDIEHQRIPTSGVLPV
                     "
BASE COUNT      156 a    169 c    153 g    179 t
ORIGIN
        1 atggacaaat ctgaatcaac cagtgctggt cgtaaccgtc ggcgtcgtcc gcgtcgtggt
       61 tcccgctccg cctcctcctc cgcggatgct aattttagag tcttgtcgca gcagctttcg
      121 cgacttaaca agacgttagc agctggtcgt ccaactatta accacccaac ctttgtaggg
      181 agtgaacgct ggaaacctgg gtacacgttc acatctatta cccttaagcc accaaaaata
      241 gaccgtgggt cttattatgg taaaaggttg ttattacctg attcagtcac ggaatatgat
      301 aagaagcttg tttcgcgcat tcaaattcga gttaatcctt tgccgaaatt tgattctacc
      361 gtgtgggtaa cagttcgtaa agttcctgcc tcctcggact tatccgttgc cgccatctct
      421 gctatgttcg cggacggagc ctcaccggta ctggtttatc agtatgctgc atctggagtc
      481 caagctaaca acaaattgtt gtatgatctt tcggcgatgc gcgctgatat aggtgacatg
      541 agaaagtacg ccgtcctcgt gtattcaaaa gacgatgcgc tcgagacaga cgagctagta
      601 cttcatgttg acatcgagca ccaacgcatt cccacgtctg gagtgctccc agtctga
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human chromosome 4 sequence-tagged site STS4-253
Date: 21 Aug 92 10:08:16 GMT
Distribution: bionet
Checksum: 52440     3

LOCUS       HUM4STS253    451 bp ds-DNA             PRI       21-AUG-1992
DEFINITION  Human chromosome 4 sequence-tagged site STS4-253
ACCESSION   L00767
KEYWORDS    STS; human chromosome 4; sequence tagged site.
SOURCE      Homo sapiens, clone p4-198 from a chromosome 4 plasmid library;
            vector pBluescript II KS + (Stratagene)
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 451)
  AUTHORS   Goold,R.D., diSibio,G., Dugaiczyk,A., Smith,K.A., Xu,H.,
            Magrane,G., Lang,D.B., Cox,D.R., Masters,S.B. and Myers,R.M.
  TITLE     Sequence-Tagged Sites for Human Chromosome 4
  JOURNAL   Unpublished (1992) see COMMENT for author address
  STANDARD  full automatic
COMMENT     PCR components: 25 ng of human genomic DNA, 10 pmol of each
            oligonucleotide, 200 micro-M dNTPs, 0.25 U Taq  polymerase (Cetus)
            in 10 micro-l of 50 mM KCl-20 mM  Tris-HCl, pH 8.3 (at room temp),
            2.5 mM MgCl-2.  Initial denaturation at 94degC for 1.5 min, then 30
            cycles of  94degC for 15 sec, 62degC for 23 sec, and 72degC for 30
            sec, followed by a final extension at 72degC for 3.5 min, using a
            Perkin-Elmer 9600 thermocycler. PCR-amplified product size 209 bp.
            Sequence submitted by:
            Human Genome Mapping Center
            Box 0925
            University of California San Francisco
            San Francisco, CA 94143-0925  USA
            Phone: (415) 502-1612   Fax: (415) 476-8391
            e-mail: hgmcprobes@cgl.ucsf.edu
FEATURES             Location/Qualifiers
     misc_feature    76..100
                     /label=Primer1
     misc_feature    complement(260..284)
                     /label=Primer2
BASE COUNT      154 a     79 c     79 g    134 t      5 others
ORIGIN
        1 gatcttttca tttcagagta tatctaccaa attcttattc tattatcaaa gataagaatt
       61 tcagttcttt actattgagc tatttaccta tgacattgtg cagntgatgc taaactttaa
      121 gtcagaggaa gtaatcattg tcaaaatctg taaaatgaaa gaaaacctca agacaacatg
      181 agtctcgata tgacagtttc tcacgtgtca acatgaatat aaaactgatg atattccaac
      241 gttgtatccg ctcttgtntg gcaaaacaaa acataatcac cacaaaaaac taagtaccta
      301 atttaagggt ataaaatgca tgtgtagtat tctgtgtagt ggaatacaag tgtaaaagaa
      361 catggactta tggattggaa aatcatgatc acttgaaccn ggtatttcca ggctgcagng
      421 agtggngatt accccgctgg actccaccct g
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Panellus stipticus ribosomal RNA (31LR6) large subunit, partial.
Date: 19 Aug 92 10:03:16 GMT
Distribution: bionet
Checksum: 49773     2

LOCUS       PNXRRLSA      246 bp ss-rRNA            RNA       19-AUG-1992
DEFINITION  Panellus stipticus ribosomal RNA (31LR6) large subunit, partial.
ACCESSION   M98624
KEYWORDS    large subunit ribosomal RNA.
SOURCE      Panellus stipticus (library: Duke culture collection 611) cDNA to
            rRNA.
  ORGANISM  Panellus stipticus
            Eukaryota; Fungi; Amastigomycota; Basidiomycetes.
REFERENCE   1  (bases 1 to 246)
  AUTHORS   Hibbett,D.S. and Vilgalys,R.
  TITLE     Phylogenetic relationships of the basidiomycete genus Lentinus
            inferred from molecular and morphological characters
  JOURNAL   Unpublished (1992)
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     rRNA            <1..>246
                     /note="Approximately aligns to region between positions
                     845 to 1093in Saccharomyces cerevisiae large subunit
                     nuclear rRNA."
                     /product="large subunit ribosomal RNA"
                     /citation=[1]
BASE COUNT       71 a     49 c     61 g     65 t
ORIGIN
        1 gaaactctgg tggaggctcg tagcgattct gacgtgcaaa tcgatcgtca aatttgggta
       61 taggggcgaa agactaatcg aaccatctag tagctggttc ctgccgaagt ttccctcaga
      121 tagcagaaac tcatatcaga tttatgtggt aaagcgaatg attagaggcc ttggggttga
      181 aacaacctta acctattctc aaactttaaa tatgtaagaa cgagccgtgc ttgattgacc
      241 gctcgg
//