From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: H.sapiens PC1 (NEC1) mRNA, complete cds.
Date: 12 Sep 92 10:02:41 GMT
Distribution: bionet
Checksum: 39228     7

LOCUS       HUMNEC1A     3298 bp ss-mRNA            PRI       12-SEP-1992
DEFINITION  H.sapiens PC1 (NEC1) mRNA, complete cds.
ACCESSION   M90753
KEYWORDS    PC1; prohormone cleavage enzyme; subtilisin-like protein.
SOURCE      Homo sapiens pituitary cDNA to mRNA.
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 3298)
  AUTHORS   Seidah,N.G., Hamelin,J., Gaspar,A.M., Day,R. and Chretien,M.
  TITLE     The cDNA sequence of the human pro-hormone and pro-protein
            convertase PC1
  JOURNAL   DNA Cell Biol. 11, 283-289 (1992)
  STANDARD  simple staff_entry
FEATURES             Location/Qualifiers
     CDS             210..2471
                     /product="PC1"
                     /gene="NEC1"
                     /codon_start=1
                     /translation="MERRAWSLQCTAFVLFCAWCALNSAKAKRQFVNEWAAEIPGGPE
                     AASAIAEELGYDLLGQIGSLENHYLFKHKNHPRRSRRSAFHITKRLSDDDRVIWAEQQ
                     YEKERSKRSALRDSALNLFNDPMWNQQWYLQDTRMTAALPKLDLHVIPVWQKGITGKG
                     VVITVLDDGLEWNHTDIYANYDPEASYDFNDNDHDPFPRYDPTNENKHGTRCAGEIAM
                     QANNHKCGVGVAYNSKVGGIRMLDGIVTDAIEASSIGFNPGHVDIYSASWGPNDDGKT
                     VEGPGRLAQKAFEYGVKQGRQGKGSIFVWASGNGGRQGDNCDCDGYTDSIYTISISSA
                     SQQGLSPWYAEKCSSTLATSYSSGDYTDQRITSADLHNDCTETHTGTSASAPLAAGIF
                     ALALEANPNLTWRDMQHLVVWTSEYDPLANNPGWKKNGAGLMVNSRFGFGLLNAKALV
                     DLADPRTWRSVPEKKECVVKDNDFEPRALKANGEVIIEIPTRACEGQENAIKSLEHVQ
                     FEATIEYSRRGDLHVTLTSAAGTSTVLLAERERDTSPNGFKNWDFMSVHTWGENPIGT
                     WTLRITDMSGRIQNEGRIVNWKLILHGTSSQPEHMKQPRVYTSYNTVQNDRRGVEKMV
                     DPGEEQPTQENPKENTLVSKSPSSSSVGGRRDELEEGAPSQAMLRLLQSAFSKNSPPK
                     QSPKKSPSAKLNIPYENFYEALEKLNKPSQLKDSEDSLYNDYVDVFYNTKPYKHRDDR
                     LLQALVDILNEEN"
     sig_peptide     210..290
                     /gene="NEC1"
                     /codon_start=1
     mat_peptide     291..542
                     /note="pro-segment"
                     /product="PC1"
                     /gene="NEC1"
                     /codon_start=1
BASE COUNT      912 a    748 c    811 g    827 t
ORIGIN
        1 gttgtcgact gtcaggaccg aagcgcttca ctgagcgctc gccgccgccc agcctctcct
       61 ctcgcgcctc ctagctcttc gcagagcaac caggagccag gagtggtcta gagcccgagg
      121 gtgggaaggg ggagtctgtc tggcttttct cctatcttgc ttctttttcc tcttcccttc
      181 ccactcttgt tcaagcgagt gtgtgagcta tggagcgaag agcctggagt ctgcagtgca
      241 ctgctttcgt cctcttttgc gcttggtgtg cactgaacag tgcaaaagcg aaaaggcaat
      301 ttgtcaatga atgggcagcg gagatccccg ggggcccgga agcagcctcg gccatcgccg
      361 aggagctggg ctatgacctt ttgggtcaga ttggttcact tgaaaatcac tacttattca
      421 aacataaaaa ccaccccaga aggtctcgaa ggagtgcctt tcatatcact aagagattat
      481 ctgatgatga tcgtgtgata tgggctgaac aacagtatga aaaagaaaga agtaaacgtt
      541 cagctctaag ggactcagca ctaaatctct tcaatgatcc catgtggaat cagcaatggt
      601 acttgcaaga taccaggatg acggcagccc tgcccaagct ggaccttcat gtgatacctg
      661 tttggcaaaa aggcattacg ggcaaaggag ttgttatcac cgtactggat gatggtttgg
      721 agtggaatca cacggacatt tatgccaact atgatccaga ggctagctat gattttaatg
      781 ataatgacca tgatccattt ccccgatatg atcccacaaa cgagaacaaa cacgggacca
      841 gatgtgcagg agaaattgcc atgcaagcaa ataatcacaa atgcggggtt ggagttgcat
      901 acaattccaa agttggaggc ataagaatgc tggatggcat tgtgacggat gctattgagg
      961 ccagttcaat tggattcaat cctggacacg tggatattta cagtgcaagc tggggcccta
     1021 atgatgatgg gaaaactgtg gaggggcctg gccggctagc ccagaaggct tttgaatatg
     1081 gtgtcaaaca ggggagacag gggaaggggt ccatcttcgt ctgggcttcg ggaaacgggg
     1141 ggcgtcaggg agataattgt gactgtgatg gctacacaga cagcatctac accatctcca
     1201 tcagcagtgc ctcccagcaa ggcctatccc cctggtacgc tgagaagtgc tcctccacac
     1261 tggccacctc ttacagcagc ggagattaca ccgaccagag aatcacgagc gctgacctgc
     1321 acaatgactg cacggagacg cacacaggca cctcggcctc tgcacctctg gctgctggca
     1381 tcttcgctct ggccctggaa gcaaacccaa atctcacctg gcgagatatg cagcacctgg
     1441 ttgtctggac ctctgagtat gacccgctgg ccaataaccc tggatggaaa aagaatggag
     1501 caggcttgat ggtgaatagt cgatttggat ttggcttgct aaatgccaaa gctctggtgg
     1561 atttagctga ccccaggacc tggaggagcg tgcctgagaa gaaagagtgt gttgtaaagg
     1621 acaatgactt tgagcccaga gccctgaaag ctaatggaga agttatcatt gaaattccaa
     1681 caagagcttg tgaaggacaa gaaaatgcta tcaagtccct ggagcatgta caatttgaag
     1741 caacaattga atattcccga agaggagacc ttcatgtcac acttacttct gctgctggaa
     1801 ctagcactgt gctcttggct gaaagagaac gggatacatc tcctaatggc tttaagaact
     1861 gggacttcat gtctgttcac acatggggag agaaccctat aggtacttgg actttgagaa
     1921 ttacagacat gtctggaaga attcaaaatg aaggaagaat tgtgaactgg aagctgattt
     1981 tgcacgggac ctcttctcag ccagagcata tgaagcagcc tcgtgtgtac acgtcctaca
     2041 acactgttca gaatgacaga agaggggtgg agaagatggt ggatccaggg gaggagcagc
     2101 ccacacaaga gaaccctaag gagaacaccc tggtgtccaa aagccccagc agcagcagcg
     2161 tagggggccg gagggatgag ttggaggagg gagccccttc ccaggccatg ctgcgactcc
     2221 tgcaaagtgc tttcagtaaa aactcaccgc caaagcaatc accaaagaag tccccaagtg
     2281 caaagctcaa catcccttat gaaaacttct acgaagccct ggaaaagctg aacaaacctt
     2341 cccagcttaa agactctgaa gacagtctgt ataatgacta tgttgatgtt ttttataaca
     2401 ctaaacctta caagcacaga gacgaccggc tgcttcaagc tctggtggac attctgaatg
     2461 aggaaaatta aaataagtgt gtggtcccaa gttggaaata ttcatgcttc ttccttaccc
     2521 tgcgattttg cctgtgtctg aagtggttgt tttgtcatga attcttatgc ttataatatc
     2581 ctttgtggca ccttttcttt ttctccctaa actgtacatg tgaaggggat gagctcaagc
     2641 aggaagttca acttccagaa ttgatcatag gtatttcaaa acacatcttt cctgtctgca
     2701 caagtgaagt gttttgttct ttctggagtc acagttgaca aaaagctctt acactacatt
     2761 agaacactgc attagagccc atttcaattc tcaaaagaaa aggcaaaacc tgggatatca
     2821 attaatttga aaacataatc tgcaaagaat gagaaggagt cagaaactgt ttctgtagct
     2881 tgttccctgt cttgtccatg tggttcttca aattttgatg ccaagaaagt atttggtagg
     2941 cctaatgaag gagttcactg taagactcat tccctagatc tttctattcc aaagtgccac
     3001 tcattcctgt agtcaaaatc tggtcatgtt ggtcaaaagc ctggattatt tagatctaga
     3061 aacagatctt gaaatctgaa tgctctggtt tgagcaattt tcgaacattc tttgcctggt
     3121 gcactgtgtc tgtggtgcca gaggcgtccg tggatccaga ggtggttatg actcgtgctg
     3181 catgcctggt ctttcctctg tttctccttc tgaaagtttt ctatacctgt ctcctttctc
     3241 agccacaaaa taaatgttgg gagaaatgat atataccact ttcccaaaaa aaaaaaaa
//