From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Chlamydomonas reinhardtii flagellar radial spoke protein (RSP4) and
Date: 4 Sep 92 10:02:46 GMT
Distribution: bionet
Checksum: 45082    17

LOCUS       CRERSP4A     7707 bp ds-DNA             PLN       04-SEP-1992
DEFINITION  Chlamydomonas reinhardtii flagellar radial spoke protein (RSP4) and
            RSP6) genes, complete cds.
ACCESSION   M87526
KEYWORDS    flagellar radial spoke protein.
SOURCE      Chlamydomonas reinhardtii (strain 21gr) (library: lambda EMBL4)
            DNA.
  ORGANISM  Chlamydomonas reinhardtii
            Eukaryota; Chlorophycota; Chlorophyceae; Volvocales;
            Chlamydomonadaceae.
REFERENCE   1  (bases 1 to 7707)
  AUTHORS   Curry,A.M., Williams,B.D. and Rosenbaum,J.L.
  TITLE     Sequence analysis reveals homology between two proteins of the
            flagellar radial spoke
  JOURNAL   Mol. Cell. Biol. 12, 3967-3977 (1992)
  STANDARD  full staff_entry
FEATURES             Location/Qualifiers
     CDS             join(611..715,868..975,1171..1217,1411..1474,1762..2023,
                     2138..2265,2414..2664,2891..3323)
                     /product="flagellar radial spoke protein"
                     /gene="RSP4"
                     /codon_start=1
                     /translation="MAAVDSVAQALAYLQVHSPQDGTSMYDHLVKLVSKVLEDQPKNA
                     VDLLETSLLVKKSTFDPKESSPLVPIPVAPDATQTQAAVSIFGDPELPINPATGEPVP
                     ADPPNEFEAENMLGAAAVLDCLGVGLGRELGVNIALAAKRIGEDPKLAVRSVRFFGKF
                     LGLYSDYFVFEVAFKKEAAKEAAPAAPAPERVEGEAASSSAPEVPVEEPGKGANKFTY
                     LVCSSLGGPLTRLPDVTPAQVKASRRIKKLLTGRLTSHVSTYPAFPGNEANYLRALIA
                     RISAATVVAPSDLFSLNDETGELERAEDWEPPAGREMAAPTAWVHVRPHLKSQGRCEV
                     HKRELPEDADEDEFYNEDELEEGPDLLAALEEDAQLPGEQAAWTPIYSSASEAVKTQA
                     GGLRSLVWPGAVCGGRGSEWTCVYVGWGVKNAPFVPLPPPPVAQEFAWGEVETQELEL
                     KPAPPPPEEEAEADE"
     mRNA            join(447..715,868..975,1171..1217,1411..1474,1762..2023,
                     2138..2265,2414..2664,2891..3696)
                     /note="other approximate transcription initiation start
                     sites are located at 458, 466, 470, and 477"
                     /product="flagellar radial spoke protein"
                     /gene="RSP4"
     CDS             join(4291..4491,4620..4666,4868..4931,5152..5273,
                     5606..5712,5930..6042,6216..6941)
                     /product="flagellar radial spoke protein"
                     /gene="RSP6"
                     /codon_start=1
                     /translation="MAADVGQALAFLQQVKTTQGASIYEGLKAALAKVLEDRPVNAVE
                     ALETSVLSTPPAANLSVPLVPAASAAAAAAAVAKASLFGDPEPVLDPESGEPIDPDAP
                     NEFECEDVEGDGDLLDGLGVGLGRQEMYAAMLAVKRLGEDAKRGVSTVRFFGKFFGTQ
                     ADYYVFETTLQSNPDMPEAPEGTIPLEPYGEGVNAYIYFVSNTLGGPLQQLPYVTPEQ
                     IKASRLLRRYLTGRLDAPVSAFPAFPGNEANYLRALIARISAATVCCPRGFFTADDDS
                     AELSANDEWVPLKGREMALPVNWSHRYAHLKGQGRTVTHKRDPPDEEEEPEKNFWTAE
                     EMEAGPPPLATLDTDAPLPAATGDKVPPPAWSPVFASASVTTRNQVAGVRSNRWPGAV
                     CACAGRHFTSMYVGWGIKAGGEWSPCPPPPPVPQWGAPAAGVEGGQQLLLECNDLPPK
                     PAPPEEEDE"
     mRNA            join(4129..4491,4620..4666,4868..4931,5152..5273,
                     5606..5712,5930..6042,6216..7598)
                     /note="Other poly-adenylation sites at 7191, 7572."
                     /product="flagellar radial spoke protein"
                     /gene="RSP6"
     exon            447..715
                     /number=1
                     /note="alternative transcription start"
                     /gene="RSP4"
     exon            458..715
                     /number=1
                     /note="alternative transcription start"
                     /gene="RSP4"
     exon            466..715
                     /number=1
                     /note="alternative transcription start"
                     /gene="RSP4"
     exon            470..715
                     /number=1
                     /note="alternative transcription start"
                     /gene="RSP4"
     exon            477..715
                     /number=1
                     /note="alternative transcription start"
                     /gene="RSP4"
     intron          716..867
                     /number=1
                     /gene="RSP4"
     exon            868..975
                     /number=2
                     /gene="RSP4"
     intron          976..1170
                     /number=2
                     /gene="RSP4"
     exon            1171..1217
                     /number=3
                     /gene="RSP4"
     intron          1218..1410
                     /number=3
                     /gene="RSP4"
     exon            1411..1474
                     /number=4
                     /gene="RSP4"
     intron          1475..1761
                     /number=4
                     /gene="RSP4"
     exon            1762..2023
                     /number=5,6
                     /gene="RSP4"
     intron          2024..2137
                     /number=5
                     /gene="RSP4"
     exon            2138..2265
                     /number=7
                     /gene="RSP4"
     intron          2266..2413
                     /number=6
                     /gene="RSP4"
     exon            2414..2664
                     /number=8
                     /gene="RSP4"
     intron          2665..2890
                     /number=7
                     /gene="RSP4"
     exon            2891..3696
                     /number=9
                     /gene="RSP4"
     polyA_signal    3679..3683
                     /gene="RSP4"
     polyA_site      3696
                     /gene="RSP4"
     exon            4129..4491
                     /note="exon 1/2"
                     /note="transcription initiation start site approximate"
                     /gene="RSP6"
     intron          4492..4619
                     /number=1
                     /gene="RSP6"
     exon            4620..4666
                     /number=3
                     /gene="RSP6"
     intron          4667..4867
                     /number=2
                     /gene="RSP6"
     exon            4868..4931
                     /number=4
                     /gene="RSP6"
     intron          4932..5151
                     /number=3
                     /gene="RSP6"
     exon            5152..5273
                     /number=5
                     /gene="RSP6"
     intron          5274..5605
                     /number=4
                     /gene="RSP6"
     exon            5606..5712
                     /number=6
                     /gene="RSP6"
     intron          5713..5929
                     /number=5
                     /gene="RSP6"
     exon            5930..6042
                     /number=7
                     /gene="RSP6"
     intron          6043..6215
                     /number=6
                     /gene="RSP6"
     exon            6216..7598
                     /number=8
                     /note="/9"
                     /gene="RSP6"
     exon            6216..7191
                     /number=8
                     /note="/9"
                     /gene="RSP6"
     exon            6216..7572
                     /number=8
                     /note="/9"
                     /gene="RSP6"
     polyA_signal    7174..7178
                     /gene="RSP6"
     polyA_site      7191
                     /gene="RSP6"
     polyA_site      7572
                     /gene="RSP6"
     polyA_site      7598
                     /gene="RSP6"
BASE COUNT     1419 a   2299 c   2558 g   1431 t
ORIGIN
        1 ccggatccag gcgtttcagg tactccttga acaggaggcg ggcctgctgg gcgctagcgc
       61 cggcttggct gccgttggtg ttctcccctt ctcaggcctg gccctgcggt gcccaacggc
      121 ggtgctccga gggcgacaag ggacggctcg cacaggctgg acaccgcaac ggcctagctg
      181 aggtccgacc cggctcgcat gttggcctat agccgccttc ttaagcccct catgaagtgg
      241 agctggcctg ccagccatcc tctgtgcaag cctcgccagt gaccgccacg gcccgcgaga
      301 gcttagccaa tgtcatccca tcgcatccca ttaaatgaac atgcatcttg tctacagcat
      361 gcagcatggg ccctaaagcc tgggtgcgcg tttgcgaagc cccgccgtac tgccattctt
      421 cgaagttgct cggcgggctg taacgaaaca ctccggctag tttcctggca ggtcaaagta
      481 tctacaaata caccagtact ctatagcttc tttcctgcac gacaatcggt agttttggca
      541 acatcgatat tgattgactg ctctcaaatt acaacgtcct tttgagcttt tgcttagctc
      601 tcttgccgca atggcggcag tggacagcgt cgcccaggcg ctggcctatt tgcaagtgca
      661 ctcgccccag gatggcacgt ctatgtatga ccacctagtc aaactagtta gcaaggtaca
      721 gctatcaccg caattgcagt atgcttgaac tggggcaagg ccatgtcgcg cggggccgcg
      781 actcatcgcc ctgcgaggct ggcgtggttg tcgggcatcg tcagcaagct atgttgagct
      841 aatactcgcg gcaaacgcgt gctgcaggtg ctggaggacc agccaaagaa tgcggtggac
      901 ctcctggaga catccctgct ggtgaagaag tccaccttcg acccgaagga gtcatccccg
      961 ctggtgccca tcccagtgcg tgggggctgg ggatgggagg aggcgagcct actgcatgaa
     1021 gctcccgtgg tgtggtgcag tactgcgtgc aacgcgtgga cgcgttaagg cccaggcgca
     1081 gagagtgggg tgcaatggga gctgtcggtg gccctgcatc gctcaggcac tgacgccgta
     1141 tgcccgggct gcccgtatta cgttacacag gtggctcctg atgcgacgca aacgcaagcg
     1201 gctgtaagca tctttgggta agttgcgttc acagcttgcg gatgcaaggg caatgccaac
     1261 atctccctca gacatgaagg cacggggttt gctggcgtca cggcggcgtc tggcagcgat
     1321 gctggctcag gggaccactg agaagtgttg gagcatgctt ccacttcgta gcttcacaat
     1381 gcggcttgcc gtggtgcggc tcacgtgcag ggacccggag ctccccatca acccagccac
     1441 gggagagccg gtgccggcgg atccgcctaa tgaggtgagg cacggagata acgcaacaga
     1501 ggaagagtgt cagtgcggat tcaaagggct ggcaggggcg cggtcttgcg agacagcgac
     1561 aggtggatgt gggcatgctc aggggctgag ctagatcact ccaggagtgc cggacccatc
     1621 cgcgccgaca atccattccc acaatccatg cacgctcgcg gcgctggcgc ggcctccttg
     1681 cctctcccct ttcgtcctgc ctaccattgc ttcgccgtaa atctcccgac ccgaacaatt
     1741 cctccctcct cggtctcaca gtttgaggcg gagaacatgc tgggcgcggc cgcggtgctg
     1801 gactgcctgg gcgtgggcct gggccgcgag ctgggcgtca acatcgcgct ggcggccaag
     1861 cgcatcggcg aggaccccaa gcttgcggtc cgatcggtgc gcttcttcgg caagttcctg
     1921 gggctgtact cggactactt cgtgttcgag gtggccttca agaaggaggc ggccaaggag
     1981 gcagcgcccg cggcgcctgc gccggagcgt gtggaagggg aaggtgaggg cagcctgggc
     2041 atcttgcgca cggttcatat tgggtactgt gtggtgtggc cattattgca ctgttcttca
     2101 tacgcgtgtg cacccctccg catcgcctgc cctgcagctg cctcctcctc cgcgcccgag
     2161 gtgccggtcg aggagccagg caagggcgcc aacaagttca cctacctggt gtgcagcagc
     2221 ctgggaggcc cgctcacacg cctgcccgac gtcacgccgg cgcaggtgcg ttgggcggcc
     2281 atgagcggcc gtgccttact accgcaacag acttgaaata gcggcacgtc ctcgcgccta
     2341 tgtccattgc gcctcaagct ctctcgctct cacgcacatt aatatcttgc tacctcgcgt
     2401 cttcacctcc caggtcaaag ccagccgccg aatcaaaaag ctgctcaccg gccgcctcac
     2461 cagccatgtg tccacctacc ccgccttccc cggcaacgag gccaactacc tgcgcgccct
     2521 cattgcgcgc atcagcgccg ccaccgtggt ggcgccctcc gacctgttct cgctcaacga
     2581 cgagaccggc gagctggagc gggcggagga ctgggagccg cccgcaggcc gcgagatggc
     2641 cgcgcccacc gcctgggtgc acgtgtgagt ctgaacggag ggagagagca cgggaggggc
     2701 ggcatgcgtg ctggaacatg cacgcctcgg ctgtagttac agttacagtg ccgcatgaag
     2761 catgtgaggt gtccttgaaa gccgaagggt ggggcgatcc gctgcgatgc actcttgtat
     2821 ctgccgtgat ccacactaaa aacggctgat ggctatactc gtgccctctc ttgtcttgtc
     2881 tcggtcgcag ccggccgcac ctgaagagcc agggccgctg cgaggtgcac aagcgcgagc
     2941 tgcccgagga cgcggacgag gacgagttct acaacgagga cgagctggag gaggggcccg
     3001 acctgctggc ggccctggag gaggacgcgc agctgcccgg cgagcaggcc gcctggacac
     3061 ccatctacag cagcgccagc gaggcggtca agacgcaggc gggcgggctg cgcagcctgg
     3121 tgtggccggg cgcggtttgc ggcggccgcg gcagcgagtg gacatgcgtg tacgtgggct
     3181 ggggcgtcaa gaacgcgccg ttcgtgccgc tgccgccgcc ccctgtggca caggagtttg
     3241 cgtggggcga ggtggagacg caggagctgg agctcaagcc cgcaccgccg ccgccggagg
     3301 aggaggccga ggcggacgag taggcggtgg tggctgtggg gaacgtgggg cgcacatggt
     3361 cgcagaagca gaggggatcg cgcggcctgt gccggtgtat ggacgcgtgg ggttatgctg
     3421 atgatgcatg gtctaaatgg ttggtcaggg tctttacttg gtgagaccgt gagaagtgca
     3481 ctgcgtggga ggggtagcac tgtggccgag agttttgcaa gcagggacca ccaagtcagg
     3541 gaatgaagta aggctgatgg tggtattcct gggctgcacg tcaacgtttc ggggacgaga
     3601 ctgcacgggg tcagtgcaag acctgccggt gcgctctgaa ctggatggaa atgcgatgag
     3661 gagaagaagg cagaacactg taaatacatg gcagttcatg agggaatgcc aaacaaggag
     3721 ttgcatcagt aacacagtgt ttgcgggtcg gggggcgaca atctgcacct tgaagcacag
     3781 cagatgccac gtacaagcac acaagattca cgacggcaac ttcaccagtc cttaacagtc
     3841 atttctggac agaatcgctg ctgtgagaga tgccctccgg ggggtgcacc agcttgccaa
     3901 caagctcggt gcggagggac gtgtcgcgag gagcgtgtcg gcgagtgcag ctctctaagc
     3961 ccaagctgca tgatgccaat cggcatgagt tcggtctcta ggtttatgtc caacctcgag
     4021 tctgcacctg cgggcatcgg ttccctggac tcactcacaa acttggattg acgagcccca
     4081 gcactatagg catgttcatg cactgctatt caattacgtg caataaactg tctttgcaac
     4141 aggaatataa accagcagct tttccttgcc ttgaatatca aaagcttgaa tactctcgat
     4201 tgcttcgtct acgcctaatt ccacgaatcg cagtttcgcc accttatcac agtagcctag
     4261 actactagtc atcttaccag gccgggcgcc atggccgcgg atgtgggcca ggctctggcc
     4321 ttcctgcagc aagtgaagac aacacaaggc gcatcaatct atgaaggcct taaagcagcg
     4381 ttggcgaagg ttctggagga tcggcctgtg aacgcggtcg aggcgctgga gacctcggtg
     4441 ctgtcgacac cccccgcagc gaacctgagt gtgccccttg ttcccgcagc tgtgagtgac
     4501 aaccgattcg tttggccgca cgggtggtgt acgtagtagt gtcatgtgtg gtgccgtctc
     4561 acgtacctgc agcctgcctg acacgctggt gtgtgttacc gtgcttcgtc gctgtgcagt
     4621 cagctgcagc tgcggcagct gccgtggcga aggcgagcct attcgggtag gccctaagct
     4681 tcgggactgg gtcctctgag gagtagctat gagattaggc aactggcagg gaatgtgatt
     4741 gccgaatggg ggacgcgtgc acccaaggtg aaaaggaggt tggcctatct ggccccgcgg
     4801 ccgtggcccg cgctcgttcc cctggtgcac gccccggctt gacgccctta tcgcgtgctc
     4861 gctgcaggga cccagagcca gtgcttgacc ccgagtcagg ggaacctatc gatccggatg
     4921 ctcccaacga ggtggggggc ttgcgagact ggggtgcaac aggtggtggc aactacagta
     4981 ttgcatggcg ttaggggtgc gcaacacggg ggccgccgca cagatgatag aaaccaggcg
     5041 ttcttgtaaa ctgcacgacc cgcgtgcctg cattgcgcca tgatccgggc cgggcattgg
     5101 tttgagggtg acctcgccct ctgtcccttc ccccgatcgt gcttaatcca gttcgagtgc
     5161 gaggacgtgg agggcgacgg tgacctgctg gacggcctgg gtgtggggct gggccggcag
     5221 gagatgtacg cggccatgct ggcggtcaag aggctggggg aggacgccaa gaggtgcgag
     5281 gcggccgggc gggcgcgggc cggggcgggg tggagcgggg gtgcggcggg gcggtctgta
     5341 cggcttgagc gattggacgc gagatggcac ggggtgcaat ggaccccgcg ccacttgggg
     5401 cgcgcgtacg cagactgtgg gcgatgagag agtacgtggg cgtgcggaga aggcgggggc
     5461 tgtgacgatt gacgaggggt gcggagaggg gcacggcagg aagtccgaat aggaatctgg
     5521 gaagcgctga gggcgctgga agcgctttac gtcaacggga ctgaaggatg ccttggcctg
     5581 gccctccccg gctgccgccg tgcaggggcg tatctacggt gcgcttcttc ggcaagttct
     5641 tcggcacgca ggccgactac tacgtgtttg agaccacgct gcagagcaac ccggacatgc
     5701 ccgaggcacc aggtgacgtc gctgcggagg gcggcggtct gcgtgtggag gaggcggctt
     5761 aggcctacag cggtgctaga gggaccgcct accgggcaaa ggggttgcgc cttcgcgcac
     5821 gcctcaatat cgtacagctg tcctccataa ccatgccccc tagctcctct ccagcattgg
     5881 gcctgcgtta tgacggtcgc cttgccccac ctcccccatc ctactaaaga gggcaccatc
     5941 cccttggagc cgtacggcga gggcgtcaac gcctacatct actttgtgtc caacacgctg
     6001 ggcggcccgc tgcagcagct gccctacgtc acgcccgagc aggtgcgtgg gcgagcgcca
     6061 ccgcacaccg cgacgcgatt gtgtgtgtag tgtgtatgct tgtggctgcg tggcgtgttt
     6121 gggccctagc cgccctgtgt gccgcagacc cgttaacatg accccgcacc gcaaactcac
     6181 gccgttccac ccacctccac ctcccaccct ccaagatcaa ggccagccgc ctgctgcgcc
     6241 gctacctgac cggccgcctg gacgcgcccg tgtccgcctt cccagccttc cccggcaacg
     6301 aggccaacta cctgcgcgcc ctcattgcgc gcatcagcgc cgccaccgtg tgctgcccgc
     6361 gcggcttctt caccgcggac gacgacagcg ccgagctgtc cgccaacgac gagtgggtgc
     6421 cgctcaaggg tcgcgagatg gcgctgcccg tcaactggtc gcaccgctac gcgcacctca
     6481 agggccaggg ccgcaccgtg acgcacaagc gcgacccgcc cgacgaggag gaggagccgg
     6541 agaagaactt ctggacggcc gaggagatgg aggccggccc gccgccgctg gccaccctgg
     6601 acacggacgc gccgctgccg gccgccactg gcgacaaggt gccaccgccc gcctggagcc
     6661 ccgtgttcgc cagcgcctcg gtgaccacgc gcaaccaggt ggcgggcgtg cgctccaacc
     6721 gctggccggg cgcggtgtgc gcctgcgcgg gccgccactt cacgtccatg tacgtgggct
     6781 ggggcatcaa ggccggcggc gagtggtcgc cctgcccgcc gccgccgccc gtgccgcagt
     6841 ggggcgcccc cgctgctggc gtcgagggcg ggcagcagct gctgctcgag tgcaacgacc
     6901 tgccgcccaa gcccgcgccg ccggaggagg aggacgagta ggcaagccca ggagggaaga
     6961 acggatcagg gcggtgtggt tagctgaggc tgaggagctg ggtgagggat gaggtgtgga
     7021 aggcgcaaag gctgcagggg gcagggaata tggtgttggt gtgcgggtcg gcggggatgc
     7081 gtcggtagag gtgggcggaa aaattgtaaa agcctgaaca tgtaagctga ggctacatga
     7141 atacagtaat acctcacctc agttatatgt gcttgtaatg gaagcaacgt tgcttgtgtg
     7201 ctggtcagca ctgatgttca ttcaacgtgt tggtgcttgc tgcccgcaat gccgtggtgt
     7261 tcctttgcgc tcatcttggg aacgtggggg acatacccgg acatggagca gccttgcggt
     7321 agtattgcct agcttttgtg ctgccttcat gcatgcgcct ttgagattgg cggctccagg
     7381 tgggatgtca ttcatgggag cagttgagtg ccagcagggc tgatgtcccc agtgccccca
     7441 ggcccaaaag ggtgtgcgtg gccgctgagg cacgaggttc atcttcatcg tttacaccac
     7501 agcacaaagc aggcgtgtca tcatgatgcc ttgagtcgtt gaactggcat ggcatgttga
     7561 cgagatgacg acaagggacc atttcaggcc aagaccgcac agatcgccct cccttggtgc
     7621 ctcgctgccc tgccctgcca acccataagc tgcgcacagc agcatggagc ccatgtgaca
     7681 tccctggaag cgccacaacc agcccaa
//