From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: B. megaterium glucose dehydrogenase (GlcDH-III) gene, complete cds.
Date: 5 Nov 92 23:05:12 GMT
Distribution: bionet
Checksum: 32721 4

LOCUS       BACGDH3      1174 bp ds-DNA             BCT       17-OCT-1992
DEFINITION  B. megaterium glucose dehydrogenase (GlcDH-III) gene, complete cds.
ACCESSION   D10625
KEYWORDS    glucose dehydrogenase.
SOURCE      Bacillus megaterium (strain IAM1030) DNA.
  ORGANISM  Bacillus megaterium
            Prokaryotae; Firmicutes; Endospore-forming rods and cocci;
            Bacillaceae.
REFERENCE   1  (bases 1 to 1174)
  AUTHORS   Mitamura,T.
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
REFERENCE   2  (sites)
  AUTHORS   Nagao,T., Mitamura,T., Wang,X., Negoro,S., Yomo,T., Urabe,T. and
            Okada,H.
  TITLE     Cloning, nucleotide sequence, and enzymatic properties of glucose
            dehydrogenase isozymes from Bacillus megaterium IAM1030
  JOURNAL   J. Bacteriol. 174, 5013-5020 (1992)
  STANDARD  full automatic
COMMENT     Submitted (28-FEB-1992) to DDBJ by: Toshihide Mitamura Department
            of Biotechnology Faculty of Engineering Osaka University 2-1
            Yamada-oka, Suita-shi Osaka 565 Japan Phone:  06-877-5111 x4373
            Fax:    06-876-2250

               [2]  sites.
FEATURES             Location/Qualifiers
     -35_signal      170..175
     -10_signal      193..198
     RBS             280..285
     CDS             294..1079
                     /EC_number="1.1.1.47"
                     /gene="gdhIII"
                     /product="glucose dehydrogenase"
                     /codon_start=1
                     /translation="MYTDLKDKVVVITGGSTGLGRAMAVRFGQEEAKVVINYYNNEEE
                     ALDAKKEVEEAGGQAIIVQGDVTKEEDVVNLVQTAIKEFGTLDVMINNAGVENPVPSH
                     ELSLDNWNKVIDTNLTGAFLGSREAIKYFVENDIKGNVINMSSVHEMIPWPLFVHYAA
                     SKGGMKQMTETLALEYAPKGIRVNNIGPGAMNTPINAEKFADPVQRADVESMIPMGYI
                     GKPEEVAAVAAFLASSQASYVTGITLFADGGMTKYPSFQTGRG"
BASE COUNT      418 a    178 c    234 g    344 t
ORIGIN      
        1 gatattcttt tcatatacat ttatttcttc ttggaaatat tcatattcaa ttaattaacc
       61 aatcaatttt aaccattgtg tgtctctagg aatgaaaaac ttcaaacaat cttttaaggt
      121 gacatgtata tggaatgaat agtgctgttt aaccatttta ctggattact aggtaaaatg
      181 agttttattt tatatattca gaggtaatct aacaagaaga caaaacttat tagttgtttt
      241 aaaaagcacc tcagaagaga gagtatattt ctgcgggata ggaggatttt attatgtata
      301 cagatttaaa agataaagta gttgtaatta caggtggatc aacaggttta ggacgcgcaa
      361 tggctgttcg tttcggtcaa gaagaagcaa aagttgttat taactattac aacaatgaag
      421 aagaagcttt agatgcgaaa aaagaagtag aagaagcagg cggacaagca atcatcgttc
      481 aaggcgacgt aacaaaagaa gaagacgttg taaatcttgt tcaaacagct attaaagaat
      541 ttggtacatt ggacgtaatg attaacaacg ctggtgttga aaacccagtt ccttctcatg
      601 agttatctct agataactgg aataaagtta ttgatacaaa cttaacaggt gcattcttag
      661 gaagccgtga agcaattaaa tatttcgttg aaaatgacat taaaggaaac gttattaaca
      721 tgtccagcgt tcacgaaatg attccttggc cattatttgt tcactacgca gcaagtaaag
      781 gcggtatgaa acaaatgacg gaaacattgg ctcttgaata tgcgccaaaa ggtatccgcg
      841 taaataacat tggaccaggt gcgatgaaca caccaattaa cgctgaaaaa ttcgctgatc
      901 ctgtacaacg tgcagacgta gaaagcatga ttccaatggg ttacatcggt aaaccagaag
      961 aagtagcagc agttgcagca ttcttagcat catcacaagc aagctatgta acaggtatta
     1021 cattatttgc tgatggcggt atgaccaaat acccttcttt ccaaactggg agaggctaaa
     1081 ggatgttttt atttatttta tgaaagaaag agtaaatatc tttattgcct gtctaaatga
     1141 taatagtgat tcagaaggaa acaataccgg attg
//