From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Cowpox virus secreted receptor binding tumor necrosis factor (crmB)
Date: 26 Jan 93 05:50:15 GMT
Distribution: bionet
Checksum: 32847 10

LOCUS       PXRCRMBX     4831 bp ds-DNA             VRL       25-JAN-1993
DEFINITION  Cowpox virus secreted receptor binding tumor necrosis factor (crmB)
            gene, complete cds; complete ORFA; complete ORFB.
ACCESSION   L08906 J02066
KEYWORDS    secreted receptor; telomere; tumor necrosis factor.
SOURCE      Poxvirus sp. (strain Brighton Red) DNA.
  ORGANISM  Poxvirus sp.
            Viridae; ds-DNA enveloped viruses; Poxviridae; Chordopoxvirinae.
REFERENCE   1  (bases 1 to 4831)
  AUTHORS   Pickup,D.J., Bastia,D., Stone,H.O. and Joklik,W.K.
  TITLE     sequence of terminal regions of cowpox virus dna: arrangement of
            repeated and unique sequence elements
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 79, 7112-7116 (1982)
  STANDARD  full automatic
REFERENCE   2  (bases 1 to 4831)
  AUTHORS   Parsons,B.L. and Pickup,D.J.
  TITLE     Transcription of orthopoxvirus telomeres at late times during
            infection
  JOURNAL   Virology 175, 69-80 (1990)
  STANDARD  full automatic
REFERENCE   3  (bases 1 to 4831)
  AUTHORS   Hu,F.-Q. and Pickup,D.J.
  TITLE     Transcription of the terminal loop region of vaccinia virus DNA is
            initiated from telomere sequences directing DNA resolution
  JOURNAL   Virology 181, 716-720 (1991)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     misc_structure  1..9
                     /citation=[1]
                     /note="part of the terminal flip-flop loop"
     misc_feature    10..95
                     /citation=[1]
                     /note="non-repeated sequence region NR1"
     misc_signal     15..19
                     /citation=[2]
                     /citation=[3]
                     /note="ATTTA transcriptional start-site of telomeric
                     transcription unit (by analogy with the sequence of
                     vaccinia virus DNA)"
     repeat_region   96..1598
                     /citation=[1]
     misc_feature    1599..1909
                     /citation=[1]
                     /note="non-repeated sequence NR2"
     misc_signal     1737..1741
                     /citation=[2]
                     /note="ATTTA - transcriptional start-site of a telomeric
                     transcription unit (by analogy with the sequence of
                     vaccinia virus DNA)"
     repeat_region   1910..2456
                     /citation=[1]
     mRNA            4608..4831
                     /gene="crmB"
     CDS             complement(2422..2619)
                     /note="ORFA"
                     /codon_start=1
                     /translation="MSRVNRAIQAHHKLKKVYRTLYSGLQNKNVLNYIHKKLPLATSS
                     FSVSICDTIMITKILNLFLME"
     CDS             complement(2648..3388)
                     /note="ORFB"
                     /codon_start=1
                     /translation="MKQIVLACICLAAVAIPTSLQQSFSSSSSCTEEENKHHMGIDVI
                     IKVTKQDQTPTNDKICQSVTEVTESEDESEEVVKGDPTTYYTVVGGGLTMDFGFTKCP
                     KISSISEYSDGNTVNARLSSVSPGQGKDSPAITREEALSMIKDCEMSINIKCSEEEKD
                     SNIKTHPVLGSNISHKKVSYEDIIGSTIVDTKCVKNLEISVRIGDMCKESSELEVKDG
                     FKYVDGSASEDAADDTSLINSAKLIACV"
     CDS             complement(3497..4564)
                     /function="secreted receptor that binds tumor necrosis
                     factor"
                     /gene="crmB"
                     /codon_start=1
                     /translation="MKSYILLLLLSCIIIINSDITPHEPSNGKCKDNEYKRHHLCCLS
                     CPPGTYASRLCDSKTNTNTQCTPCASDTFTSRNNHLPACLSCNGRCDSNQVETRSCNT
                     THNRICDCAPGYYCFLKGSSGCKACVSQTKCGIGYGVSGHTPTGDVVCSPCGLGTYSH
                     TVSSVDKCEPVPSNTFNYIDVEINLYPVNDTSCTRTTTTGLSESISTSELTITMNHKD
                     CDPVFRNGYFSVLNEVATSGFFTGQNRYQNISKVCTLNFEIKCNNKDSYSSSKQLTKT
                     KNDDDSIMPHSESVTLVGDCLSSVDIYILYSNTNTQDYETDTISYHVGNVLDVDSHMP
                     GRCDTHKLITNSNSQYPTHFL"
BASE COUNT     1660 a    650 c    953 g   1568 t
ORIGIN      
        1 attttactaa ttttatttag tgtctagaaa aaaatgtgta acccatgact gtaggaaact
       61 ctagagggta agaaagatcg atcgctttat agagaccatc agaaagaggt ttaatatttt
      121 tgtgagacca ttgaagagag aaagagaaag agaataaaaa tattttagtg actccatcag
      181 aaagaggttt aatatttttg tgagaccatt gaagagagaa agagaaagag aataaaaata
      241 ttttagtgac tccatcagaa agaggtttaa tatttttgtg agaccatcga agagagaaag
      301 agaataaaaa tatttttgta aaactttttt atgagaccat tgaagagaga aagagaataa
      361 aaatattttt gtaaaacttt tttatgagac cattgaagag agaaagagaa taaaatattt
      421 ttacgactcc atcagaaaga ggtttaatat ttttgtgaga ccattgaaga gagaaagaga
      481 ataaaaatat ttttgtaaaa cttttttatg agaccattga agagagaaag agaataaaaa
      541 tatttttgta aaactttttt atgagaccat tgaagagaga aagagaataa aatattttta
      601 cgactccatc agaaagaggt ttaatatttt tgtgagacca ttgaagagag aaagagaata
      661 aaaatatttt tgtaaaactt ttttatgaga ccattgaaga gagaaagaga ataaaaatat
      721 ttttgtaaaa cttttttatg agaccattga agagagaaag agaataaaat atttttacga
      781 ctccatcaga aagaggttta atatttttgt gagaccattg aagagagaaa gagaataaaa
      841 atatttttgt aaaacttttt tatgagacca ttgaagagag aaagagaata aaaatatttt
      901 tgtaaaactt ttttatgaga ccattgaaga gagaaagaga ataaaatatt tttacgactc
      961 catcagaaag aggtttaata tttttgtgag accattgaag agagaaagag aataaaaata
     1021 tttttgtaaa acttttttat gagaccatcg aagagagaaa gagaataaaa atatttttgt
     1081 aaaacttttt tatgagacca ttgaagagag aaagagaata aaatattttt acgactccat
     1141 cagaaagagg tttaatattt ttgtgagacc attgaagaga gaaagagaat aaaaatattt
     1201 ttgtaaaact tttttatgag accattgaag agagaaagag aataaaaata tttttgtaaa
     1261 acttttttat gagaccattg aagagagaaa gagaataaaa tattttacga ctccatcaga
     1321 aagaggttta atatttttgt gagaccattg aagagagaaa gagaataaaa atatttttgt
     1381 aaaacttttt tatgagacca ttgaagagag aaagagaata aaatatttta cgactccatc
     1441 agaaagaggt ttaatatttt tgtgagacca ttgaagagag aaagagaata aaatatttta
     1501 cgactccatc agaaagaggt ttaatatttt tgtgagacca ttgaagagag aaagaggttt
     1561 aatatttttg tgagaccatt gaagagagaa agagaaatag tctagatatt tttcttagta
     1621 caaaagtcaa tgttttaaaa tatatggaca agaatttgtc tgtataaaaa cttgtgtgaa
     1681 attttgtacc aaagaaaaaa cgtgagcagt atcccctaca tggattttac tagatcattt
     1741 atataccaaa aaatattata cgatctacgt tttattatat gattttaacg tgtaaattat
     1801 aaacattatt ttatgatgca attgtctggt aacctagatg ggcataggga tgttgataag
     1861 ctctacgagt atatgttgtt ggacgttatc gtttacgaaa tagttgagac atcagaaaga
     1921 gaataaaaat atttttacga ctccatcaga aagaggttta atatttttgt gagaccattg
     1981 aagagagaaa gagaataaaa atatttttgt aaaacttttt ttatgagacc attgaagaga
     2041 gaaagagaat aaaatatttt tacgactcca tcagaaagag gtttaatatt tttgtgagac
     2101 cattgaagag agaaagagaa taaaaatatt tttgtaaaac tttttttatg agaccattga
     2161 agagagaaag agaataaaat atttttacga ctccatcaga aagaggttta atatttttgt
     2221 gagaccattg aagagagaaa gagaataaaa atatttttgt aaaacttttt ttatgagacc
     2281 attgaagaga gaaagagaat aaaaatattt ttgtaaaact ttttttatga gaccattgaa
     2341 gagagaaaga gaataaaaat atttttgtaa aacttttttt atgagaccat tgaagagaga
     2401 aagagaataa aaatattttt gttactccat cagaaagagg tttaatattt tagtgatcat
     2461 aatcgtatca catattgaga cagaaaaaga agaagtcgcg agaggtaact ttttgtgaat
     2521 gtagttaaga acatttttgt tttgcaaacc ggaatatagt gtccggtaca ctttttttag
     2581 tttgtggtgt gcctgaatcg ctcgattaac cctactcatc caatttcaga tgaatagggt
     2641 tatcgattca gacacacgct ataagttttg ctgaattgat gagtgaagta tcatcggctg
     2701 catcttccga tgccgatccg tcgacatact tgaatccatc cttgacctca agttcagatg
     2761 attccttgca catgtctcct atacgaacgc taatctctag attcttgacg catttcgtat
     2821 cgacgatcgt tgatccgatg atatcttcgt aactcacttt cttatgggag atgttagacc
     2881 cgagtactgg atgggtcttg atgttgctgt ctttctcttc ttcgctacat ttgatgttga
     2941 tagacatttc acagtctttg atcatagaca gagcttcttc acgagtgata gcgggagagt
     3001 ccttaccttg tcctggggac acgctagaca atctagcatt cacagtgttt ccgtcagagt
     3061 attcagagat ggatgaaatc tttgggcatt tggtgaatcc aaagtccatc gtaagacctc
     3121 caccgacgac agtgtaataa gtggtgggat ctccctttac gacttcttct gactcgtctt
     3181 cagactctgt aacttcggtt actgattgac aaatcttatc attggtcggt gtttggtctt
     3241 gcttggtgac tttgataata acatcgattc ccatatgatg tttgttttct tcttccgtac
     3301 acgaggatga ggatgagaat gattgctgaa gactggtagg gatagcaact gccgccaggc
     3361 atatgcatgc caggacgatt tgtttcatga ttgctgttct agatgattct atttcctcac
     3421 catacaataa attagaatat attttctact tttacgaaaa attaattatt gtatttattt
     3481 atgggttaaa aacttactat aaaaagtggg tgggatactg ggaattggaa ttagtaatca
     3541 gtttatgtgt atcgcaccta ccgggcatat ggctatcgac atcgagaaca ttacccacat
     3601 gataagagat tgtatcagtt tcgtagtctt gagtattggt attactatat agtatataga
     3661 tgtcgacgct ggatagacag tcgcccacta gagttaccga ttccgaatgc ggcatgatgg
     3721 agtcgtcatc attctttgtt ttcgttaact gtttggagga agaataagaa tctttgttat
     3781 tacatttaat ctcgaaattc agagtgcata cctttgaaat attctgatat ctattttgtc
     3841 ctgtaaagaa ccctgaagtt gctacctcat taagaacgga gaagtatcca ttacgaaaga
     3901 cgggatcgca gtctttatga ttcatagtaa tcgttagttc cgaagttgag atggattcac
     3961 tgagaccggt agtggtcgtc cgagtacacg atgtgtcgtt gacgggatac agattaattt
     4021 ccacatcgat atagttaaag gtattactgg gtacgggttc gcatttatct acggaagaga
     4081 cggtgtgaga atatgttccg agaccacacg gagaacagac gacgtctccg gtaggcgtgt
     4141 gtccggatac tccgtatcct attccacact ttgtttggga aacacatgcc ttgcatccgg
     4201 atgatccttt gagaaaacaa taatatccgg gagcacaatc acagattcta ttgtgagtcg
     4261 tgttacacga tcgcgtctct acctgattac tatcgcatct tccgttacaa cttagacaag
     4321 cgggtaaatg attattgcga gacgtaaagg tgtccgacgc acacggcgta cattgtgtgt
     4381 ttgtgttagt cttgctatcg cataatctgg aagcgtatgt tcccggagga cacgataaac
     4441 aacatagatg atggcgtttg tattcgttgt ctttacactt tccgttggat ggttcatgcg
     4501 gtgttatatc gctgtttatt atgattatac atgaaagcag caatagcaat atatatgact
     4561 tcatgattta ataatgtagt agtaattatc acctcgttcc cgctctccaa tcattataga
     4621 tattttttaa atattttcat ttcatttcat ttcatttcat ttcatttcat ttcatttcat
     4681 ttcatttcat ttcatttcat ttctagtatg gataatgttt gtaatggttc tttccgtaca
     4741 acatactttt tagatgatag tcgcttaact tggatatgat gctgcgtata atttctggag
     4801 gcaaataata tagtctagat tgaccatcga t
//