From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Leishmania major aldoketo reductase gene, complete cds.
Date: 12 Sep 92 10:02:45 GMT
Distribution: bionet
Checksum: 31793     5

LOCUS       LEIHMTXR     2242 bp ds-DNA             INV       12-SEP-1992
DEFINITION  Leishmania major aldoketo reductase gene, complete cds.
ACCESSION   L01699
KEYWORDS    aldoketo reductase; methotrexate resistance.
SOURCE      Leishmania major (strain Lt 252) promastigote DNA.
  ORGANISM  Leishmania major
            Eukaryota; Animalia; Protozoa; Sarcomastigophora; Mastigophora;
            Kinetoplastida; Trypanosomatina; Trypanosomatidae.
REFERENCE   1  (bases 1 to 2242)
  AUTHORS   Callahan,H.L.
  JOURNAL   Unpublished (1992)
  STANDARD  full staff_review
REFERENCE   2  (bases 1 to 2242)
  AUTHORS   Callahan,H.L. and Beverley,S.M.
  TITLE     A member of the aldoketo reductase family confers methotrexate
            resistance in Leishmania
  JOURNAL   Unpublished (1992)
  STANDARD  full staff_entry
FEATURES             Location/Qualifiers
     CDS             562..1425
                     /function="methotrexate resistance"
                     /product="aldoketo reductase"
                     /codon_start=1
                     /translation="MTAPTVPVALVTGAAKRLGRSIAEGLHAEGYAVCLHYHRSAAEA
                     NALSATLNARRPNSAITVQADLSNVATAPVSGADGSAPVTLFTRCAELVAACYTHWGR
                     CDVLVNNASSFYPTPLLRNDEDGHEPCVGDREAMETATADLFGSNAIAPYFLIKAFAH
                     RSRHPSQASRTNYSIINMVDAMTNQPLLGYTIYTMAKGALEGLTRSAALELAPLQIRV
                     NGVGPGLSVLVDDMPPAVWEGHRSKVPLYQRDSSAAEVSDVVIFLCSSKAKYITGTCV
                     KVDGGYSLTRA"
BASE COUNT      391 a    735 c    616 g    500 t
ORIGIN
        1 ccactgctgt gtcgtagatc ctcgagaaag tgtaggcgat cttgttgcat gtcggtagtg
       61 cagtaccgcg aaactgccat gcttgttgca gacgagcgct tcttgctctc ttacgcgcgc
      121 gccgcgaagc gtgcccttca ctgacgaaat accccctccg cccactcgtg cttctctcac
      181 ggccgccgcc ccgcaccctg tctctctcgt cgcctgtttt cgctgcagtc gcccagtgtg
      241 ccacgtgcac ctacaccgca atcgttgtgt tgtctgaggt ttgaacccgc tgcctgcgct
      301 ttcttttcgg atttcatgat gccatcatcg tcgccgaagc cacgcacaca ccgttgacgc
      361 gaacgttcgc cgttgtttca catctcgccc ccacccctcc catcgccttt atctccgtaa
      421 cgccgcacta tgtagccagt cgactttttt aactgcatcg agacgctccg ctcgtgagga
      481 ttccctgttg ctgcgcaagg ggtttctgtt tcccttctgt gcactgctta atcgccgagg
      541 agctgaattc gctgtgccac gatgactgct ccgaccgtgc cggtggcgtt ggtaacaggc
      601 gccgcgaagc gtcttggccg cagtatcgct gagggactcc acgcggaggg gtacgctgtc
      661 tgcttgcact atcatcgctc tgctgcagaa gcgaacgcac tatccgcgac gctcaacgca
      721 aggcgaccga acagcgccat cacggtgcag gcggatctga gcaacgttgc cacagccccg
      781 gtcagcggcg ctgatggctc tgcacctgtt accctcttca cgcgctgtgc tgagttggtg
      841 gctgcgtgct acacccactg gggacgctgc gacgtgctag tgaacaacgc ctcttctttc
      901 taccccacgc cgctgctgag gaatgacgag gatggacacg agccctgtgt cggagataga
      961 gaggcaatgg agacggccac cgctgacctc ttcggctcca acgcgatagc gccctacttc
     1021 ttgattaagg cgttcgcgca tcgctcgcgg caccccagcc aagcatcgcg caccaactac
     1081 tccatcatca acatggtcga cgccatgacg aaccagcctc ttctcgggta caccatatat
     1141 accatggcca aaggggcgtt ggaggggctg acacggtctg ccgcgctgga gcttgcgccg
     1201 ctgcagattc gagtgaacgg cgttggtccg ggtttgtcgg tgctcgtcga tgacatgccc
     1261 cctgctgtgt gggagggcca ccgcagcaag gtgcctctgt accagcgcga ttcctccgcc
     1321 gcagaggtga gcgacgttgt tatctttctg tgctcctcca aggccaagta catcaccggc
     1381 acctgtgtca aagtggatgg tggctacagc cttacccggg cctgagcgcg gcgcatgaca
     1441 gccacaaccc cagcgaatct cgtgtcggcg gaagcaccct caatttctac gcgcgcgcgt
     1501 tttctttttt tcttttgcgt gtgggaacaa cgacccgcgc aatcgggact cctcacgccc
     1561 gcccccctcg cccccccccg cagcctcttc cgcaccggcc gcgcgaacgc agtcgaggag
     1621 aaaggggggg gcgaggggcg cacacgttgc agctaaagcg ccgagggacg gcaatgacag
     1681 agagagacgg caacaagcga aagaacaaag gagccgaaag aagtgagcgc agccatgtgt
     1741 atgcgtgaag cagtccggtg aggccaagcg tgcgcgcgac ttcctcggca ctcctatttc
     1801 ttcctcctcc gttattggtg tctggtgcca cggccgacag cgttcaagga cgactcgcct
     1861 ctgcgggtat taagatgagg caggtacgct cttcgtctct tgctggcttg tctggcttcc
     1921 atcacccctg tgtgtcttac gcccacaccg caccgggtgg tacgggcgtc tctctctctc
     1981 tctctgcgtg tgtgtatgca tgtcaccgcc tatgtatcgc tgtcgtagcg gcaccagact
     2041 tcgcccttct tcgctgtgat gtgccgtgcc atttacggct tcgaccttca cttgcctccg
     2101 cctactttct ctctctgtga acctgaataa cgggcactct atcgcctccg tgtgggctat
     2161 tcaagtcgac acagggcggg accagcactc ttgggcaacg tctcccagcc ctccccctca
     2221 agcatcttgc tcaggtccgt ag
//