From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Bacillus subtilis ISB8 modification endonuclease type II (hsmBI)
Date: 11 Sep 92 10:02:20 GMT
Distribution: bionet
Checksum: 46453     7

LOCUS       BACHSMR      2892 bp ds-DNA             BCT       11-SEP-1992
DEFINITION  Bacillus subtilis ISB8 modification endonuclease type II (hsmBI)
            gene, complete cds, restriction endonuclease (hsrBI) gene, complete
            cds.
ACCESSION   L01541
KEYWORDS    hsmBI gene; hsrBI gene; modification endonuclease type II;
            restriction endonuclease.
SOURCE      Bacillus subtilis (strain ISB8) DNA.
  ORGANISM  Bacillus subtilis
            Prokaryotae; Firmicutes; Endospore-forming rods and cocci;
            Bacillaceae.
REFERENCE   1  (bases 1 to 2892)
  AUTHORS   Xu,G., Kapfer,W., Walter,J. and Trautner,T.A.
  TITLE     BsuBI-an isospecific restriction and modification system of PstI:
            Characterization of the BsuBI genes and enzymes
  JOURNAL   Unpublished (1992)
  STANDARD  full staff_entry
FEATURES             Location/Qualifiers
     -10_signal      185..190
                     /gene="hsmBI"
     mRNA            198..>1733
                     /gene="hsmBI"
     CDS             228..1733
                     /product="modification endonuclease type II"
                     /gene="hsmBI"
                     /codon_start=1
                     /translation="MTQILETVDKSRLTVNPLLKNKSELGQFFTPSSISIFMACLFSE
                     DKLNNAKVLDAGAGIGSLTSAFLARLISENIGKADLHLLEIDEMLEPYLSETLALFKD
                     YIEINSQIIIDDFIEWAAYSLLDEESLLAKDKQRFTHAILNPPYKKIKSNSKHRKLLR
                     KAGIETVNLYSAFVALTVDLMSDGGEIVFIIPRSFCNGPYFRHFRQHLLNKTSIKHMH
                     LFESRDKAFKDDEVLQENVISKLEKGTVQEDVKISISTDDSFSVIRSYRYPFEKIVQP
                     NDIEKFIHINTTNEETLIEKHPNVCYSLEELNIEVSTGPVVDFRVKENLREMPGEGTV
                     PLFYPNHFVGTSLEYPKMMKKPNAIIRNEKVEKWLYPNGHYVVVKRFSSKEEKRRIVA
                     GVLTPESVNDPVVGFENGLNVLHYNKSGISKEVAYGLYAYLNSTPVDKYFRIFNGHTQ
                     VNATDLRTMKFPSRDILISLGKWVIENIENVGQVEIDSKLEELLLNDRGNA"
     RBS             1697..1701
                     /gene="hsrBI"
     CDS             1714..2664
                     /product="restriction endonuclease"
                     /gene="hsrBI"
                     /codon_start=1
                     /translation="MTEGMHSNVKEAIKILKELGLPKGQQNERSALCLLSLMNITQDK
                     TWSEAESPLIGITPMMEFCRINYGKEYAPNSRETFRRFTMHQFVDAGIALYNPDKPTR
                     PVNSPKAVYQIEAETLELIKCYNTEEWSELLARYLSNRQTLVERYAKERQQNKIPVQI
                     AEGKEIYITPGEHSELIKAIIEEFAPRYVPGGRLIYAGDTGEKMGYFDEELLRQLGVV
                     IDSHGKMPDVVIYFPEKKWLLLIESVTSHGPVDHKRHEELAKLFNGSTAGIVYVTAFP
                     NRSLMARYLNNISWETEVWVADAPSHLIHFNGVRFLGPYE"
BASE COUNT     1032 a    419 c    602 g    839 t
ORIGIN
        1 cagctgagaa aaaagaattt ttaaaaatat aggcattgaa gagatacgtg taaaatagaa
       61 aagtatatcg aatttaaaag cattgaagaa tgaaaaaaga acatgtctag actatctcta
      121 attgtgataa accctgctga aaagtaccag cagggtttat tgtgttaatc attacaaaaa
      181 atgataaaat aaaaaagcaa gacgtatcaa tattaaggag tgctaccatg actcaaattc
      241 tagaaacagt tgataaatca agattaactg ttaacccatt acttaagaat aagtctgagt
      301 tgggtcaatt ttttacacct tcaagtattt ctatatttat ggcttgcttg tttagtgaag
      361 ataaattaaa taatgccaaa gttttggatg cgggagcagg aataggatct ctaactagtg
      421 ccttcttggc tcgattaatt tcagaaaaca ttggtaaagc tgatcttcat ttgttggaaa
      481 tcgatgaaat gttagagcct tatttgtctg aaaccctggc tttattcaaa gattatattg
      541 aaattaattc acaaattatt attgatgatt ttattgagtg ggcagcgtat agtttactgg
      601 atgaagagag tttactcgca aaagataagc agcggtttac tcatgctatc ttaaatccgc
      661 catataagaa aataaaaagc aattctaaac atagaaaact tttaagaaaa gcaggaattg
      721 aaactgtaaa cttatattca gcatttgtag ctctgacagt tgatttaatg tctgatggtg
      781 gtgaaatagt ttttattata ccaaggagct tctgtaatgg cccttatttt cgtcatttta
      841 gacaacactt attgaacaaa acctcaatta aacatatgca tttgtttgaa tctagggaca
      901 aggcatttaa ggatgatgaa gttttacaag aaaatgtcat aagtaagcta gaaaaaggaa
      961 ctgtgcaaga agatgttaaa atatctatct caacagatga tagcttttct gttataagga
     1021 gttataggta tccattcgag aaaattgtcc aaccaaatga tattgaaaag tttattcata
     1081 taaacacaac caatgaggaa actcttatag agaagcatcc aaatgtttgt tattccttgg
     1141 aggaactaaa tattgaggtt tcaacaggac cagttgtaga tttcagagta aaagaaaatc
     1201 taagagaaat gccaggagaa ggaactgttc cattgtttta tccaaatcac tttgttggga
     1261 caagtttaga gtaccccaag atgatgaaaa agcctaatgc aatcattaga aatgaaaagg
     1321 ttgaaaagtg gctttaccct aatgggcatt atgttgttgt taaaaggttt tcttcaaaag
     1381 aagaaaaacg tcgaatagta gcaggagtat taacaccaga gagtgtaaac gatccagtag
     1441 ttggttttga gaacggactc aatgtacttc actataataa gagtggaatt tctaaagaag
     1501 tagcgtatgg tctttatgcc tatcttaact cgaccccagt agataagtat ttcagaattt
     1561 ttaatggtca tactcaggta aatgctactg accttagaac aatgaaattt cctagtagag
     1621 atatcctaat aagtttaggt aaatgggtaa tagaaaatat agaaaatgta gggcaggttg
     1681 aaatagatag taagttggag gagttgttat taaatgaccg agggaatgca tagtaacgtc
     1741 aaagaagcaa taaaaattct taaagaatta ggtttgccta aggggcagca aaatgaacgt
     1801 tcagctctat gtttattatc tttaatgaat ataactcaag ataagacatg gtcagaagct
     1861 gaaagtcctc ttattggtat aacaccaatg atggaatttt gccgaattaa ttatggaaaa
     1921 gaatatgcac caaatagtcg agaaactttt agaaggttca ctatgcatca gtttgtagat
     1981 gctggaattg ctttgtataa ccctgataaa ccgacaagac ctgttaatag tccgaaggca
     2041 gtatatcaaa tagaagcgga aactttagaa cttattaagt gctataatac agaagaatgg
     2101 agtgaattac tagcccgata cttatctaat agacaaacat tggtagaaag gtatgctaaa
     2161 gagagacagc agaataaaat acctgtacaa atagctgaag gtaaagagat ttatataact
     2221 ccaggagagc acagcgagtt aattaaggca attatagaag aatttgcacc ccgttatgta
     2281 ccaggaggca gattaattta tgctggtgat actggtgaaa agatgggtta ttttgatgaa
     2341 gaattattaa gacagttagg ggtagttata gactcgcatg gaaaaatgcc tgatgtagta
     2401 atttattttc cagagaaaaa gtggctccta ctcattgaat cggtaactag tcacggtcca
     2461 gtagaccata agcgtcatga agaattagcc aaattattta atggttcaac tgcgggaata
     2521 gtttatgtga ctgcattccc taatcgttca ttaatggcaa gatacctaaa taacatttct
     2581 tgggagacgg aagtgtgggt agctgatgca ccgtcacatc taattcattt taatggcgtt
     2641 cgtttcttag gcccatacga gtaattttaa tttgactctc ttataaataa ttggaaaaaa
     2701 gatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga
     2761 agcgagcagg actggcggcg gccaaagcgg tcggacagtg ctcgagaacg ggtgcgcata
     2821 gaaattgcat caagcatata ggcctagacg cagcccatag tgactggcga tgctgtcgga
     2881 atggacgata tc
//