From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Trypanosoma brucei (clone WaTat1.14) surface glycoprotein (VSG)
Date: 24 Jul 92 10:10:32 GMT
Distribution: bionet
Checksum: 12684     6

LOCUS       TRBSURGLYD   1685 bp ss-mRNA            INV       24-JUL-1992
DEFINITION  Trypanosoma brucei (clone WaTat1.14) surface glycoprotein (VSG)
            mRNA, complete cds.
ACCESSION   M83697
KEYWORDS    surface glycoprotein.
SOURCE      Trypanosoma brucei cDNA to mRNA.
  ORGANISM  Trypanosoma brucei
            Eukaryota; Animalia; Protozoa; Sarcomastigophora; Mastigophora;
            Kinetoplastida; Trypanosomatina; Trypanosomatidae.
REFERENCE   1  (bases 1 to 1685)
  AUTHORS   Barbet,A.F., Davis,W.C. and McGuire,T.C.
  TITLE     Cross-neutralization of two different trypanosome populations
            derived from a single organism
  JOURNAL   Nature 300, 453-456 (1982)
  STANDARD  full automatic
REFERENCE   2  (bases 1 to 1685)
  AUTHORS   Barbet,A.F., Myler,P.J., Williams,R.O. and McGuire,T.C.
  TITLE     Shared surface epitopes among trypanosomes of the same serodeme
            expressing different variable surface glycoprotein genes
  JOURNAL   Mol. Biochem. Parasitol. 32, 191-200 (1989)
  STANDARD  full staff_review
REFERENCE   3  (bases 1 to 1685)
  AUTHORS   Kamper,S.M. and Barbet,A.F.
  TITLE     Surface epitope variation via mosaic gene formation is potential
            key to long-term survival of Trypanosoma brucei.
  JOURNAL   Mol. Biochem. Parasitol. 53, 33-44 (1992)
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     sig_peptide     74..157
                     /standard_name="VSG"
                     /product="surface glycoprotein"
                     /gene="VSG"
                     /evidence=EXPERIMENTAL
                     /codon_start=1
                     /citation=[2]
                     /citation=[3]
     CDS             74..1600
                     /standard_name="VSG"
                     /product="surface glycoprotein"
                     /gene="VSG"
                     /evidence=EXPERIMENTAL
                     /codon_start=1
                     /translation="MTKRPTKLSQVALSYLLLIAVSPPHVQPASQSPLDKSLWEGICK
                     AAAQLNKVPGTMTHELTQILQKAKTMRRGELKAQIFGLRHAGTELANRAAVMAAYFSR
                     RYKQIIEALETQDIEKAIDAAAKAAYLQGRIDDPLHLLAAVENTNNLCLSTTGATGNK
                     PVKTEGKLGDVDCPLSAPSAKNKAAELSGITEAGFTMLKTDVSSGDAKQVASGAKKCN
                     LLSSVNGQGFGQTQAAISTDHEILGGYITIKNTNTEIDVTAHNKAHTVESGKHESWTA
                     AHAAIMVSPKHTTTPYINETGEIHSRSDMTKVLQAVYSAKPPISNTGLENKVIEIFQG
                     KEAKKLQDYEDAVNQVTIPAGIAGLETDQTLAAVNDEDKLIAIRAFYEQQVGAAYGKM
                     VATISEASNKQTATAPADCGKKLKKVDCKDSDGCKWTNDKEETGNHCKAKDGEGKTNT
                     AAAAGTNSEGKKCSDKKSEVVCKDGCKWDGTECKDSSILLTKNFARSVVSAALVALLF
                     "
                     /citation=[2]
                     /citation=[3]
     mat_peptide     158..1528
                     /standard_name="VSG"
                     /product="surface glycoprotein"
                     /gene="VSG"
                     /evidence=EXPERIMENTAL
                     /codon_start=1
                     /citation=[2]
                     /citation=[3]
BASE COUNT      606 a    399 c    393 g    287 t
ORIGIN
        1 aacgctatta ttagaacagt ttctgtacta tattgcagcc aagaaacaca gaaaagctag
       61 ccatcaaaag aagatgacaa aaaggccaac gaaactgagc caagtagcgc tttcatactt
      121 gttactaatc gccgtatcgc cgcctcatgt ccaacccgct agtcaatcac cattggacaa
      181 aagcctctgg gagggcatat gcaaagcggc ggcgcagctg aacaaagttc ccgggacgat
      241 gacgcacgaa ttgacacaga tcctacagaa agcgaagacg atgcggcgtg gcgaactgaa
      301 agcacaaata tttgggctaa ggcatgcagg aacagaattg gccaacagag cagcggtaat
      361 ggcggcgtat ttcagccgta ggtacaagca gataattgaa gcgcttgaga cgcaagacat
      421 cgaaaaagcc atcgacgcgg cagccaaagc agcgtacctt caaggcagaa tagacgaccc
      481 gcttcattta ctggcagctg tggaaaacac caacaacctt tgtctgtcta cgaccggagc
      541 gacaggcaac aaaccagtca aaacagaggg caaactcggc gacgtcgact gcccactgtc
      601 ggcgccgtca gctaaaaaca aggcagccga attaagcggt ataacagagg ccggctttac
      661 catgctaaaa acggatgtgt caagcggcga cgccaaacaa gtcgcgagtg gcgcaaagaa
      721 atgtaacctt ctaagttcag taaacggcca aggcttcgga cagacacagg cagcgatcag
      781 caccgaccac gaaattctcg gaggatacat aacaataaaa aacacaaaca ctgaaataga
      841 cgtaaccgcg cacaacaaag cacacacagt cgaaagcggc aagcacgaaa gctggacggc
      901 ggcacatgca gccattatgg tgagcccaaa acacacaacg acaccctata tcaatgaaac
      961 tggggaaatt cacagcaggt cagacatgac aaaagtgttg caagccgttt acagcgcaaa
     1021 accgccgata agcaacactg gcctagagaa caaggtcata gaaatatttc aaggaaaaga
     1081 agcaaagaaa ctgcaagact acgaagacgc tgtaaaccaa gtaacgatac cagccggcat
     1141 agcaggactg gaaactgacc agacgctagc agcagtaaac gacgaagaca aactaatcgc
     1201 aatcagagcc ttttatgaac agcaggtcgg cgcagcctat ggcaaaatgg tagcgacgat
     1261 atccgaagca agcaataaac aaacagccac cgccccagca gactgcggta aaaaactaaa
     1321 gaaagtagac tgcaaagaca gtgatggttg caaatggact aacgataagg aagaaacagg
     1381 aaaccactgc aaagctaaag atggagaagg aaaaacaaat acagcagcag ctgcaggaac
     1441 aaattcagaa ggcaaaaagt gctccgataa gaaaagtgaa gtcgtgtgca aagatggctg
     1501 caaatgggac ggaacagagt gcaaagattc ctctattctc ctaacaaaga acttcgcccg
     1561 cagcgtcgtt tctgctgcgt tagtggcctt gctgttttaa aatttttccc cctcaatttt
     1621 taaaagaaat ttgctaaaaa ttttgctact tgaaaacatt ctgatatatt ttaacacctt
     1681 taaat
//