From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human immunodeficiency virus type 1, isolate, SIMI, clone pULB5384
Date: 29 Dec 92 00:50:38 GMT
Distribution: bionet
Checksum: 54071 7

LOCUS       HIVSIMI84    2571 bp ss-DNA             VRL       04-DEC-1992
DEFINITION  Human immunodeficiency virus type 1, isolate, SIMI, clone pULB5384
            complete env cds.
ACCESSION   L07421
KEYWORDS    .
SOURCE      Human immunodeficiency virus type 1 (HIV-1), isolate SIMI, clone
            pULB5384, PCR amplification of proviral DNA from short-term
            cocultured PBMCs.
  ORGANISM  Human immunodeficiency virus type 1
            Viridae; ss-RNA enveloped viruses; Positive strand RNA virus;
            Retroviridae; Lentivirinae.
REFERENCE   1  (sites)
  AUTHORS   Bex,F., Vanhulle,C., Sprecher,S., Geissendorfer,M.,
            Rubsamen-Waigmann,H. and Burny,A.
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
COMMENT     Kindly provided prior to publication in computer-readable form by
            F. Bex and A. Burny, University of Brussels,   Belgium. The
            sequence in this entry is one of 2 clone sequences over the env
            gene obtained from a Belgian patient with AIDS. The sequencing work
            was done by the EEC Centralized Facility for HIV genome
            characterization, Georg-Speyer-Haus, Frankfurt,Germany.  A vaccinia
            virus recombinant expressing this env gene was constructed and used
            to vaccinate the non-infected patient's brother (identical twin)
            with the aim of immune therapy by adoptive transfer of lymphocytes
            (publication in preparation).
FEATURES             Location/Qualifiers
     CDS             1..2571
                     /product="envelope polyprotein"
                     /codon_start=1
                     /translation="MRVKGIRKNCQHLWRWGTMLLGMLMICSAVEQLWVTVYYGVPVW
                     KEATTTLFCASDAKAYDTEVHNVWATHACVPTDPSPQEIVLENVTENFNMWKNNMVKQ
                     MHEDIISLWDQSLKPCVKLTPLCVTLNCTNWKSANSTSNTNSSLEEKGEIKNCSFNVT
                     TSIKNKVQTEYALFYKLDVMPIEDDNTSYTLINCNTSVITQACPKVSFEPIPIHYCTP
                     AGFAILKCNDKKFNGTGPCKNVSTVQCTHGIKPVVSTQLLSNGSLSEGEVVIRSENFT
                     DNAKTIIVQLNKSVVINCTRPNNSKRKTLHMGPKRAFYATGDIGGYTGYIRQAHCNIS
                     RAKWNDTLKQIVKKLKEQFGRNKTIVFKQSSGGDPEIVMHSFNCGGEFFYCNSTKLFN
                     STWNDTSTWNDTTGSNKGNITLLCRIKQIINRWQEVGKAMYAPPIEGHIRCSSNITGL
                     LLTRDGGKNESETTETFRPGGGDMRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQRE
                     KRAVGLIGALFLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQSNLLRAIEAQQHLL
                     QLTVWGIKQLQARVLAVERYLKDQQLLGIWGCSGKLVCTTTVPWNASWSNKSLNQIWD
                     NMTWMEWEREIDNYTGLIYRLIEESQNQQEQNEQDLLKLDTWASLWNWFSITKWLWYI
                     KIFIMIVGGLIGLRIIFAVLSIVNRVRQGYSPLSFQTLLPAPRGPDRPEGIEEEGGER
                     DRDRSGQLVTGFLPIIWGDLRSLFLFSYHRLRDLLLIVARIVELLGRRGWEILKYWWN
                     LLQYWSQELKNSAVSLLNTTAIVVAEGTDRVIEVVQRIGRAVLHIPRRIRQGFERALL
                     "
BASE COUNT      892 a    434 c    616 g    629 t
ORIGIN      
        1 atgagagtga aggggatcag gaagaattgt cagcacttgt ggagatgggg caccatgctc
       61 cttgggatgt tgatgatctg tagtgctgta gaacaattgt gggttacagt ctattatggg
      121 gtacctgtgt ggaaagaagc aaccaccacc ctattttgtg catcagatgc taaagcatat
      181 gacacagagg ttcataatgt ttgggccaca catgcctgtg tacccacaga ccctagccca
      241 caagaaatag tattggaaaa tgtgacagaa aattttaata tgtggaagaa taacatggta
      301 aaacagatgc atgaggatat aatcagttta tgggatcaaa gcctaaagcc atgtgtaaaa
      361 ttaaccccac tctgtgttac tttaaattgc actaattgga agagtgctaa tagtaccagt
      421 aataccaata gtagtttgga ggagaaagga gaaataaaaa actgctcttt caatgtcacc
      481 acaagtataa aaaataaggt acagacagaa tatgcacttt tttataaact tgatgtaatg
      541 ccaatagagg atgataatac aagctataca ttgataaatt gtaacacctc agtcattaca
      601 caagcctgtc caaaggtatc ctttgaacca attcccatac attattgtac cccagctggt
      661 tttgcgattc taaagtgtaa tgataagaag ttcaatggaa caggaccatg taaaaatgtc
      721 agcacagtac aatgcacaca tggaattaag ccagtagtgt caactcaact gctgtcaaat
      781 ggcagtctat cagaaggaga ggtagtaatt agatctgaaa atttcactga caatgctaaa
      841 accataatag tacagctgaa taaatctgta gtaattaatt gtacaagacc caacaacagt
      901 aaaagaaaaa ctctacatat gggaccgaag agggcatttt atgcaacagg agacatagga
      961 ggatatacag gatatataag acaagcacat tgtaacatta gtagagcaaa atggaatgac
     1021 actttaaaac agatagttaa aaaattaaaa gaacaatttg ggaggaataa aacaatagtc
     1081 tttaagcaat cctcaggagg ggacccagaa attgtaatgc acagctttaa ttgtggaggg
     1141 gaatttttct actgtaattc aacaaagctg tttaatagta cttggaatga tactagtact
     1201 tggaatgata ctacaggatc aaataaagga aatatcacac tcctatgcag aataaaacaa
     1261 attataaaca ggtggcaaga agtaggaaaa gcaatgtatg cccctcccat cgaaggacat
     1321 attagatgtt catcaaatat tacagggctg ttactaacaa gagatggtgg taagaacgag
     1381 agcgagacca ccgagacctt cagacctgga ggaggagata tgagggataa ttggagaagt
     1441 gaattataca aatataaagt ggtgaagatt gagccattag gagtagcacc caccaaggca
     1501 aagagaagag tggtgcagag agaaaaaaga gcagtgggac tgataggagc tttgttcctt
     1561 ggattcttgg gagcagcagg aagcactatg ggcgcggcgt caatgacgct gacggtacag
     1621 gccagacaat tattgtctgg tatagtgcaa cagcagagca atttgctgag ggctattgag
     1681 gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca ggcaagagtc
     1741 ctggctgtgg aaagatacct aaaggaccaa cagctcctag ggatttgggg ttgctctgga
     1801 aaactcgttt gcaccactac tgtgccttgg aatgctagtt ggagtaataa atccctgaat
     1861 cagatttggg ataacatgac ctggatggag tgggagagag aaattgacaa ttacacaggc
     1921 ttaatataca gattaattga agaatcgcag aaccaacagg aacagaatga acaagactta
     1981 ttgaaattgg atacgtgggc aagtttgtgg aattggttta gcataacaaa atggctgtgg
     2041 tatataaaaa tattcataat gatagtagga ggtttaatag gtttaagaat aatttttgct
     2101 gtactttcta tagtgaatag agttaggcag ggatactcac cattatcatt tcagaccctc
     2161 ctcccagccc cgaggggacc cgacaggccc gaaggaatcg aagaagaagg tggagagaga
     2221 gacagagaca gatccggaca attagtgact ggattcttac caatcatctg gggcgaccta
     2281 cggagcctgt tcctcttcag ctaccaccgc ttgagagact tactcttgat tgtagcgagg
     2341 attgtggaac ttctgggacg cagggggtgg gaaatcctca aatactggtg gaatctcctg
     2401 cagtattgga gtcaggaact aaagaatagt gctgttagct tgcttaatac aactgctata
     2461 gtagtagctg aggggacaga tagggttata gaagtagtac aaagaattgg tagagctgtt
     2521 ctccacatac ctagaagaat aagacagggc ttcgaaaggg ctttgctata a
//