From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human mRNA for osteopontin.
Date: 5 Nov 92 23:12:21 GMT
Distribution: bionet
Checksum: 34174 5

LOCUS       HUMOP        1424 bp ss-mRNA            PRI       15-MAR-1990
DEFINITION  Human mRNA for osteopontin.
ACCESSION   X13694
KEYWORDS    osteopontin; sialoprotein I.
SOURCE      human (Homo sapiens).
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 1424)
  AUTHORS   Kiefer,M.C.
  JOURNAL   Unpublished (1988) see COMMENT for author address
  STANDARD  full automatic
REFERENCE   2  (bases 1 to 1424)
  AUTHORS   Kiefer,M.C., Bauer,D.M. and Barr,P.J.
  TITLE     The cDNA and derived amino acid sequence for human osteopontin
  JOURNAL   Unpublished (1988) see COMMENT for author address
  STANDARD  full automatic
COMMENT     [1]  enum. 1 to 1424. [1]  enum. 1 to 1424.
               *source: clone=Op-30; library=lambda ZAP, ost3cDNA; *source:
            tissue=osteosarcoma; The sequence overlaps with that reported by
            Oldberg et. al.  in Proc. Natl. Acad. Sci. USA 83:8819-8823(1986)
            M14656.

              EMBL features not translated to GenBank features:
              key        from     to       description

              SITE        302    304       glycosylation site
              SITE        383    385       glycosylation site
               [1]  Author address Kiefer M.C., Chiron Corporation, 4560 Horton
            Street, Emeryville, CA 94608, USA.
               Submitted (05-DEC-1988) on tape to the EMBL data library by:
FEATURES             Location/Qualifiers
     sig_peptide     68..115
                     /note="signal peptide (AA -16 to -1)"
                     /codon_start=1
     mat_peptide     116..1009
                     /note="mature osteopontin (AA 1 to 298)"
                     /codon_start=1
     CDS             68..1012
                     /product="osteopontin"
                     /codon_start=1
                     /translation="MRIAVICFCLLGITCAIPVKQADSGSSEEKQLYNKYPDAVATWL
                     NPDPSQKQNLLAPQNAVSSEETNDFKQETLPSKSNESHDHMDDMDDEDDDDHVDSQDS
                     IDSNDSDDVDDTDDSHQSDESHHSDESDELVTDFPTDLPATEVFTPVVPTVDTYDGRG
                     DSVVYGLRSKSKKFRRPDIQYPDATDEDITSHMESEELNGAYKAIPVAQDLNAPSDWD
                     SRGKDSYETSQLDDQSAETHSHKQSRLYKRKANDESNEHSDVIDSQELSKVSREFHSH
                     EFHSHEDMLVVDPKSKEEDKHLKFRISHELDSASSEVN"
BASE COUNT      449 a    293 c    295 g    387 t
ORIGIN      
        1 gaccagactc gtctcaggcc agttgcagcc ttctcagcca aacgccgacc aaggaaaact
       61 cactaccatg agaattgcag tgatttgctt ttgcctccta ggcatcacct gtgccatacc
      121 agttaaacag gctgattctg gaagttctga ggaaaagcag ctttacaaca aatacccaga
      181 tgctgtggcc acatggctaa accctgaccc atctcagaag cagaatctcc tagccccaca
      241 gaatgctgtg tcctctgaag aaaccaatga ctttaaacaa gagacccttc caagtaagtc
      301 caacgaaagc catgaccaca tggatgatat ggatgatgaa gatgatgatg accatgtgga
      361 cagccaggac tccattgact cgaacgactc tgatgatgta gatgacactg atgattctca
      421 ccagtctgat gagtctcacc attctgatga atctgatgaa ctggtcactg attttcccac
      481 ggacctgcca gcaaccgaag ttttcactcc agttgtcccc acagtagaca catatgatgg
      541 ccgaggtgat agtgtggttt atggactgag gtcaaaatct aagaagtttc gcagacctga
      601 catccagtac cctgatgcta cagacgagga catcacctca cacatggaaa gcgaggagtt
      661 gaatggtgca tacaaggcca tccccgttgc ccaggacctg aacgcgcctt ctgattggga
      721 cagccgtggg aaggacagtt atgaaacgag tcagctggat gaccagagtg ctgaaaccca
      781 cagccacaag cagtccagat tatataagcg gaaagccaat gatgagagca atgagcattc
      841 cgatgtgatt gatagtcagg aactttccaa agtcagccgt gaattccaca gccatgaatt
      901 tcacagccat gaagatatgc tggttgtaga ccccaaaagt aaggaagaag ataaacacct
      961 gaaatttcgt atttctcatg aattagatag tgcatcttct gaggtcaatt aaaaggagaa
     1021 aaaatacaat ttctcacttt gcatttagtc aaaagaaaaa atgctttata gcaaaatgaa
     1081 agagaacatg aaatgcttct ttctcagttt attggttgaa tgtgtatcta tttgagtctg
     1141 gaaataacta atgtgtttga taattagttt agtttgtggc ttcatggaaa ctccctgtaa
     1201 actaaaagct tcagggttat gtctatgttc attctataga agaaatgcaa actatcactg
     1261 tattttaata tttgttattc tctcatgaat agaaatttat gtagaagcaa acaaaatact
     1321 tttacccact taaaaagaga atataacatt ttatgtcact ataatctttt gttttttaag
     1381 ttagtgtata ttttgttgtg attatctttt tgtggtgtga ataa
//