From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Trypanosoma brucei (clone WaTat1.13) surface glycoprotein (VSG)
Date: 24 Jul 92 10:10:31 GMT
Distribution: bionet
Checksum: 54639     6

LOCUS       TRBSURGLYC   1682 bp ss-mRNA            INV       24-JUL-1992
DEFINITION  Trypanosoma brucei (clone WaTat1.13) surface glycoprotein (VSG)
            mRNA, complete cds.
ACCESSION   M83696
KEYWORDS    surface glycoprotein.
SOURCE      Trypanosoma brucei cDNA to mRNA.
  ORGANISM  Trypanosoma brucei
            Eukaryota; Animalia; Protozoa; Sarcomastigophora; Mastigophora;
            Kinetoplastida; Trypanosomatina; Trypanosomatidae.
REFERENCE   1  (bases 1 to 1682)
  AUTHORS   Barbet,A.F., Davis,W.C. and McGuire,T.C.
  TITLE     Cross-neutralization of two different trypanosome populations
            derived from a single organism
  JOURNAL   Nature 300, 453-456 (1982)
  STANDARD  full automatic
REFERENCE   2  (bases 1 to 1682)
  AUTHORS   Barbet,A.F., Myler,P.J., Williams,R.O. and McGuire,T.C.
  TITLE     Shared surface epitopes among trypanosomes of the same serodeme
            expressing different variable surface glycoprotein genes
  JOURNAL   Mol. Biochem. Parasitol. 32, 191-200 (1989)
  STANDARD  full staff_review
REFERENCE   3  (bases 1 to 1682)
  AUTHORS   Kamper,S.M. and Barbet,A.F.
  TITLE     Surface epitope variation via mosaic gene formation is potential
            key to long-term survival of Trypanosoma brucei.
  JOURNAL   Mol. Biochem. Parasitol. 53, 33-44 (1992)
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     sig_peptide     67..150
                     /standard_name="VSG"
                     /product="surface glycoprotein"
                     /gene="VSG"
                     /evidence=EXPERIMENTAL
                     /codon_start=1
                     /citation=[2]
                     /citation=[3]
     CDS             67..1608
                     /standard_name="VSG"
                     /product="surface glycoprotein"
                     /gene="VSG"
                     /evidence=EXPERIMENTAL
                     /codon_start=1
                     /translation="MTKRPTNLNGAMLCSLLLLAVSPPHVQPASQSPLDKSLWQGICK
                     ATAELNKVPGAMTNELTQILHRAKTMRRGELKAQIFGLRHAGTPSANKAAVMAAYFSR
                     RYKQIIEALETQGIEKAIDAAAKAAYLQGRIDDPLHLLAAVENTNNLCLSMTGAEGNK
                     PVKTEGKLGDVDCPLSAPSAKNKAAELSGITEAGFTMLKTDVTSGNAKQVASGAKKCN
                     LLSSVNTQGFGETQAAISTDHEILGGYITIKNTNTEIDVTAHNKAHTVESGKHESWTV
                     AHAAIMVSPKHTTSPYANETGEIHSRPDMTKVLQAVYSAKPPISGTDLENKVIEIFQG
                     KEEKKLQDYEDAVNQVTIPAGVAGLETDQTLAAVNDEDKLIAIRAFYEQQVSAAYGKM
                     VATIAEASNKQTATAPADCGKKLKKVDCKESDGCKWNSTDKSEGDFCKAKGEDEQKAQ
                     GAGTGDGAAGEQKKEDKCKDKKKDDCKSPDCKWEGETCKDSSFLLNKKFALTVVSAAF
                     AALLF"
                     /citation=[2]
                     /citation=[3]
     mat_peptide     151..1536
                     /standard_name="VSG"
                     /product="surface glycoprotein"
                     /gene="VSG"
                     /evidence=EXPERIMENTAL
                     /codon_start=1
                     /citation=[2]
                     /citation=[3]
BASE COUNT      605 a    402 c    406 g    269 t
ORIGIN
        1 cagtttctgt actatattga gaaaaaagga gccaagaaac acagaaaagc tagccatcaa
       61 aagaagatga caaaaaggcc aacgaacctg aacggagcca tgctttgttc attgttactg
      121 ctagccgtat cgccgcctca tgtccaaccc gctagtcaat caccattgga caaaagcctc
      181 tggcagggca tatgcaaagc gacggcagag ctgaacaaag ttccgggcgc gatgacgaac
      241 gaattgacgc agatcctaca cagggcgaag acgatgcggc gtggcgaact gaaagcacaa
      301 atatttgggc taaggcatgc aggaacacca tcggcaaaca aagcagcggt aatggcggcg
      361 tatttcagcc gtaggtacaa gcagataatt gaagcgcttg aaacgcaagg catcgaaaag
      421 gcaatcgacg cggcagccaa agcagcgtac cttcagggca gaatagacga ccccctacac
      481 ttactggcag ctgtggaaaa caccaacaac ctttgtctat ctatgaccgg tgcggaaggc
      541 aacaaaccag tcaaaacaga gggcaaactc ggcgacgtcg actgcccact gtctgcgccg
      601 tcagctaaaa acaaggcagc cgaattaagc ggtataacag aggccggctt taccatgcta
      661 aaaacggatg tgaccagcgg caacgcaaaa caggtcgcga gcggcgcaaa gaaatgcaac
      721 ctactaagtt cagtaaacac ccaaggcttc ggagagacac aggcagcgat cagcaccgac
      781 cacgaaattc tcggaggata cataacaata aaaaacacaa acactgaaat agacgtaacc
      841 gcgcacaaca aagcacacac agtcgaaagc ggcaagcacg aaagctggac ggtggcacat
      901 gcagccatta tggtgagccc aaaacacaca acgagcccct atgccaacga aactggagag
      961 attcacagca ggccggacat gacaaaagtg ttgcaagccg tttacagcgc aaagccgccg
     1021 ataagcggca ccgacctaga gaacaaggtc atagaaatat ttcaaggaaa agaagaaaag
     1081 aaactgcaag actacgaaga cgctgtaaac caagtaacga taccagccgg cgtagcagga
     1141 ctggaaactg accagacgct agcagcagta aacgacgaag acaaactaat cgcaatcaga
     1201 gccttttatg aacagcaggt cagcgcagcc tatggcaaaa tggtagcgac gatagccgaa
     1261 gcaagcaata aacaaacagc caccgcccca gcagactgcg gtaaaaaact aaagaaagta
     1321 gactgcaaag aaagcgatgg atgcaagtgg aatagcactg acaaaagtga aggagacttc
     1381 tgcaaagcta aaggggaaga tgaacaaaaa gcccaaggag caggaacagg agatggagct
     1441 gcaggagaac aaaagaaaga ggataaatgc aaagataaga aaaaggatga ctgcaaatct
     1501 ccggattgta aatgggaggg cgaaacttgc aaagattcca gcttcctcct aaacaagaaa
     1561 ttcgccctca ccgtggtttc tgctgcattt gcggccttgc ttttttaaaa caaaaatttc
     1621 cccctccttt tcttaaagaa aattttgcta cttgaaaaac tttctgatat attttaacac
     1681 ct
//