From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: H.roretzi muscle actin gene.
Date: 3 Jan 93 22:15:07 GMT
Distribution: bionet
Checksum: 51806 6

LOCUS       HROMA4A      2339 bp ds-DNA             INV       23-DEC-1992
DEFINITION  H.roretzi muscle actin gene.
ACCESSION   D10887
KEYWORDS    muscle actin.
SOURCE      H.roretzi DNA, clone lambda ACT11.
  ORGANISM  Halocynthia roretzi
            Eukaryota; Animalia; Chorqata; Tunicata; Ascidiacea;
            Stolidobranchia; Pyuridae.
REFERENCE   1  (bases 1 to 2339)
  AUTHORS   Kusakabe,T.
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
REFERENCE   2  (sites)
  AUTHORS   Kusakabe,T., Makabe,K.W. and Satoh,N.
  TITLE     Tunicate muscle actin genes: structure and organizarion as a gene
            cluster
  JOURNAL   J. Mol. Biol. 227, 955-960 (1992)
  STANDARD  full automatic
COMMENT     Submitted (19-May-1992) to DDBJ by: Takehiro Kusakabe Department of
            Zoology Faculty of Science Kyoto University Oiwake-cho,
            Kitashirakawa Sakyo-ku, Kyoto 606-01 Japan Phone:  075-751-6149
            Fax:    075-753-4081

               [2]  sites.
FEATURES             Location/Qualifiers
     misc_feature    459..468
                     /note="CArG box"
     TATA_signal     545..551
     prim_transcript 575..2199
     exon            575..1107
                     /number=1
     intron          1108..1315
                     /number=1
     exon            1316..1669
                     /number=2
     intron          1670..1773
                     /number=2
     exon            1774..2199
                     /number=3
     polyA_signal    2182..2187
     CDS             join(651..1107,1316..1669,1774..2099)
                     /gene="HrMA4a"
                     /product="muscle actin"
                     /codon_start=1
                     /translation="MSDGEEDTTAIVCDNGSGLVKSGFAGDDAPRAVFPSIVGRPRHQ
                     GVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPE
                     EHPTLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQAVLSLYASGRTTGIVLDAGD
                     GVSHNVPIYEGYALPHAIARLDLAGRDLTDYLMKILTERGYSFVTTAEREIVRDIKEK
                     LCYVALDFEQEMATAASSTSLEKSYELPDGQVITIGNERFRCPETLFQPSFIGMESAG
                     IHETTYNSIMKCDIDIRKDLYANNVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIA
                     PPERKYSVWIGGSILASLSTFQQMWISKQEYDEAGPSIVHRKCF"
BASE COUNT      724 a    487 c    426 g    702 t
ORIGIN      
        1 attacatgat ataaaataca gacatgaaaa tcattatccg aatggtactt gacattaaaa
       61 taaaaaacgt actgaaactt acgaccagca tctttatgcc ccaactttca tctgacctct
      121 gcttaaagac tagtgtttga ttgtccctgg tttagaaaaa gttcgttgac caaatttttc
      181 gaagttggag gcggaggaaa ccatccaaaa ttgcacgtgt agaaattcat ctggtccaat
      241 aaaaattatt accaattagg aattgtgaat aggattgcat ttcatcaact aatacctgca
      301 taaaaaatta ttactaattt gtgaacagac cgcaaatatt ctttactgga cttcgctgct
      361 gttattcacg acaccaaggc cgcccaagtg tagaactcaa ttgttcgcac gtaccgccag
      421 ttgaacgata tatcccacca tgctattctt aagttactcc ttttatggtc atcgcacttc
      481 tcattacgaa ttgcgcgtga taacaactgt cttcattttt tccgctttcc tccgtttctt
      541 tcagtatata agccctctat cgttcgctta tattcatcac caagcttgta cagtcaatca
      601 atcaattaca ccttctaagt tctatctgta ctaaataaca aaacaaaatc atgtctgatg
      661 gtgaagaaga tacaaccgca atcgtctgcg acaatggatc aggattggtc aagtctggtt
      721 ttgccggtga tgacgcacca cgagcagttt ttccgtcaat cgttggtcgt ccaagacatc
      781 aaggtgttat ggtcggcatg ggacagaagg acagctacgt aggagatgag gctcaaagca
      841 aaagaggaat cctcactttg aaatatccca ttgaacatgg tatcatcaca aactgggatg
      901 atatggaaaa gatctggcat cacaccttct ataatgaact ccgtgtcgct cctgaagaac
      961 atccaactct tctcacagaa gctccgttga atcccaaggc aaatagagag aagatgacac
     1021 aaattatgtt cgagaccttc aacgtcccag ccatgtacgt cgccattcaa gctgtgctat
     1081 cactatatgc ttctggaaga acaactggta agtgtatgac aattggtaat gtagtttgtt
     1141 cgtataaatc aacaaaatat gtcacactcc gttttgaaca aatcgtaata gcaggggttg
     1201 tcagtgtgga tttttcaagg cgaggtctag ggaaaatact tgcattatca tatattgatt
     1261 tgattctgtt taaaaataca ttagctacta tcaaatgatt tctgtacgat tataggtatt
     1321 gtattggatg ctggtgatgg tgtctcccac aacgtcccca tctacgaagg atacgctctt
     1381 cctcatgcta tcgcaaggtt agatttggcc ggtcgtgatc taactgatta cttgatgaag
     1441 attctcacag aaagaggata ttctttcgtt actactgccg aacgtgaaat cgttcgtgat
     1501 atcaaggaaa agttatgcta cgtcgcactc gacttcgaac aagagatggc aactgcagct
     1561 tcgtctactt cactcgaaaa gagctacgag cttcccgatg gacaagttat cactattgga
     1621 aacgaaagat tccgttgccc ggaaactctt ttccagcctt cattcattgg taagttgaaa
     1681 gcttcatcat ctgttcatca acgaagatat acaatatttc ctttgcgatt ccgatttcat
     1741 aagcgacata ctaatgaata tgttatttgt caggtatgga atcagctggt attcatgaaa
     1801 ccacgtacaa ttccatcatg aaatgcgata ttgacatccg taaggatctt tacgctaaca
     1861 acgttctctc tggtggaacc accatgtatc ctggtattgc tgatcgtatg caaaaagaaa
     1921 tcacagccct tgctccgagt accatgaaga tcaaaatcat tgctcctcct gaacgtaaat
     1981 attctgtctg gattggagga tcgatccttg cttccctctc taccttccaa cagatgtgga
     2041 ttagcaaaca agaatacgat gaagccggac catccattgt tcatagaaag tgtttctaat
     2101 tcacatctcc agctatgcac cattcactgt acggacttaa attattacca tttccatgaa
     2161 ttgccgtttg ttatgatgtc aaataaaatt cgactctgta aaaaattgaa tatgaatttt
     2221 cttaatttct tgaagaggga aattacattt catagatata atatgattac actattccga
     2281 aatatagtga gtaaataaac atttcattca ataaatgatg ctacatggaa taattaaaa
//