From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human cytomegalvirus polyhydrophobic transmembrane protein (US20,
Date: 3 Jan 93 22:15:17 GMT
Distribution: bionet
Checksum: 46692 8

LOCUS       HS5POLYTRP   3158 bp ds-RNA             VRL       23-DEC-1992
DEFINITION  Human cytomegalvirus polyhydrophobic transmembrane protein (US20,
            US19, and US18) genes, complete cds's.
ACCESSION   L04998
KEYWORDS    polyhydrophobic transmembrane protein.
SOURCE      Human cytomegalovirus (strain Towne) (library: lambda gt11) cDNA to
            genomic RNA.
  ORGANISM  Human cytomegalovirus
            Viridae; ds-DNA enveloped viruses; Herpesviridae;
            Betaherpesvirinae.
REFERENCE   1  (bases 1 to 3158)
  AUTHORS   Guo,Y.-W. and Huang,E.-S.
  TITLE     Characterization of a structually tricistronic gene of human
            Cytomegabovine composed of US18, US19 and US20
  JOURNAL   J. Virol. (1992) In press
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     repeat_unit     56..70
                     /rpt_type=INVERTED
     repeat_unit     75..80
                     /rpt_type=INVERTED
     repeat_unit     214..219
                     /rpt_type=INVERTED
     repeat_unit     218..223
                     /rpt_type=DIRECT
     mRNA            240..3158
                     /note="2.85 kDa"
     repeat_unit     271..277
                     /rpt_type=INVERTED
                     /label=IR4
     repeat_unit     358..363
                     /rpt_type=INVERTED
     repeat_region   371..377
                     /rpt_type=DIRECT
     repeat_unit     401..406
                     /rpt_type=INVERTED
     TATA_signal     450..456
                     /note="beta transcript; 2.85 kDa"
     repeat_unit     515..521
                     /rpt_type=DIRECT
     repeat_unit     532..537
                     /rpt_type=DIRECT
     TATA_signal     1273..1279
                     /note="minor transcipt"
     repeat_unit     1760..1766
                     /rpt_type=DIRECT
     repeat_unit     1834..1840
                     /rpt_type=INVERTED
     repeat_unit     1872..1879
                     /rpt_type=DIRECT
     repeat_unit     1928..1933
                     /rpt_type=DIRECT
     CAAT_signal     2085..2093
     TATA_signal     2115..2121
     mRNA            2143..3158
                     /gene="US18"
     polyA_signal    3137..3142
     polyA_site      3158
     CDS             240..1268
                     /gene="US20"
                     /note="43 kDa"
                     /product="polyhydrophobic transmembrane protein"
                     /codon_start=1
                     /translation="MRVISRARSACTWTSCTSLSPCSTSCPPSPAAPTLLRRRSLPQQ
                     RRRPSSSPNRRVRGVTTSPCPTRSLVYKRRVGAPQRLCAETVATMQAQEANALLLSRM
                     EALEWFKKFTVWLRVYAIFIFQLAFSFGLGSVFWLGFPQNRNFCVENYSFFLTVLVPI
                     VCMFITYTLGNEHPSNATVLFIYLLANSLTAAIFQMCSESRVLVGSYVMTLALFISFT
                     GLAFLGGRDRRRWKCISCVYVVMLLSFLTLALLSDADWLQKIVVTLCAFSISFFLGIL
                     AYDSLMVIFFCPPNQCIRHAVCLYLDSMAIFLTLLLMLSGPRWISLSDGVPLDNGTLT
                     AASTTGKS"
     CDS             1315..2037
                     /gene="US19"
                     /product="polyhydrophobic transmembrane protein"
                     /codon_start=1
                     /translation="MLHVVPLEWTVEEVVPYLERLAVWLRASVLVAFQLTATVALSVL
                     SWWLMPPPVAELCERGRDDDPPPLSHLSLVVPVGCLFLLLRGPSIDRCPRKLPLLLAY
                     CLPHALAFLTLLMCQPSPQAFVGAALLALAVDLSCLGASLLGCDPGASLRRLWLPSVL
                     SLLCATALGLWLLRAAAPFFLGLHATTLLTVTLMLIHDLSLITCQSSFPESFQPSLRL
                     YVENVALFIGMYHLLRLWLWSP"
     CDS             2175..2999
                     /gene="US18"
                     /note="36 kDa"
                     /codon_start=1
                     /translation="MGDTASVSEHHESPTVTIVPLHRSHALVAEQQLFQWLKRFKLLM
                     EVYHGLVWQLACTLTVCLLAWLAFPDVQGQCANGIVPALSSIVPVSTLAMLRGFAEFR
                     PHTTNFAHLTVACLLINTGITVCTGFCGERRVIGLSFALVMVFFVLCSGLTYLAGNNP
                     TRWKVIGIGYGWSVIVFYLLLYFSPVLWVSKIYSGLYVLVVTAASAVLIYETLDLIYQ
                     RGTLSKNSVCVSVVLYTIVMSLLNMSVAIFSGHVWVQQYAEKHGGRIDGVSLLSLL"
BASE COUNT      518 a    983 c    850 g    807 t
ORIGIN      
        1 catggtggcc acagtaacgc tattttgttt ccttacgctc gctacgtatc tgtttgcccg
       61 cgacgtggaa ttacagcgtt ccctattgac gggcgcatcc acgctgattc tattactgtt
      121 cgccgtattt tctctttttc ccgaggcggt aagtgagatc ctggtgatga tagccggttt
      181 ggccgtcatc gtgacctcgg tggtgtgcga tacgcaggac atcctgcacg acatcgagta
      241 tgagagttat atcccgggcg cgctctgctt gtacatggac ctcatgtacc tctttgtctc
      301 cgtgctctac ttcatgccct ccgagcccgg cagcgcccac actgctcaga cgacggtcgc
      361 tgccacagca gcggcgtcgc cccagttcgt ctcctaatcg acgtgtccgc ggcgtgacca
      421 cgtcaccgtg ccccactcgc tcgcttgtgt ataagagaag ggtaggtgcg ccgcagcggc
      481 tttgtgccga gaccgtcgcc accatgcagg cgcaggaggc taacgcgctg ctgctctccc
      541 gcatggaggc tctcgagtgg ttcaaaaagt tcaccgtatg gctgcgcgtg tacgccatct
      601 tcatctttca gctggctttc agcttcggct tgggaagcgt tttttggttg gggttcccac
      661 aaaaccgcaa cttttgcgtc gagaactaca gcttctttct caccgtgctc gtgcccatcg
      721 tctgcatgtt catcacgtac acgttgggca acgaacaccc tagtaacgcc acggtgcttt
      781 tcatctatct gttggccaac agcctgacgg cggccatctt ccagatgtgc tctgaaagcc
      841 gcgtactagt aggttcctac gtgatgaccc tggcgttgtt tatctccttt acggggctgg
      901 cgtttctagg tggccgtgac cgacgtcgct ggaaatgcat cagctgcgtc tacgtggtga
      961 tgctgctttc gttcctcacg ctcgctctgc taagcgacgc cgattggctg cagaagatag
     1021 tggtgacgtt gtgcgccttc tctatcagct tctttttggg tattctggcc tacgacagtc
     1081 tcatggtcat ctttttctgc ccacctaacc aatgcatccg tcacgccgtc tgtctctacc
     1141 tggacagcat ggccatcttt ctcacgttgt tgctcatgct ctcgggtccc cgttggatta
     1201 gtctttcgga cggcgtgcct ttggacaacg ggactttgac agccgccagt acgacgggga
     1261 agtcctaatg cctataaaac cgcgcccgtt ttcacagcag ccgcgcttgt cgccatgctt
     1321 catgtcgtcc cgctagaatg gaccgtcgag gaggtagtcc cctacctaga gaggctggcg
     1381 gtatggctgc gcgcctccgt cctcgtcgcc ttccagctta ccgccaccgt cgccctcagc
     1441 gtgctcagct ggtggctcat gccgccaccg gtggccgagc tatgcgagcg gggacgggac
     1501 gacgacccac cgccgctttc tcatctcagc ctcgtcgtgc ccgtcggctg cctgttcctg
     1561 ctgctacgcg gtccgtcgat cgaccgttgt ccccgcaaac tcccactcct gttggcttac
     1621 tgcctgccgc acgcgctagc ctttctgacc ttgctgatgt gccagccgtc gccgcaggcc
     1681 tttgtcggcg cggctcttct cgccctggcg gtagatctca gctgcctggg ggcctcgctt
     1741 ttgggttgcg atcctggcgc gtctctccgg cggctgtggc taccgtcggt cctgtctctg
     1801 ctctgcgcta cggcgctggg actttggctg ctgcgggccg cggccccgtt ctttctcggt
     1861 ttacacgcga cgacgctgtt gacggtgacg ctgatgctaa ttcacgatct gtccctgatc
     1921 acctgtcaga gttctttccc ggagagtttt cagccctcgc tacgcctcta tgtcgaaaat
     1981 gtggctttat tcatcggcat gtaccatctt ctgaggctct ggttgtggag cccatgacga
     2041 cgggtcggat acagggtgcc gccacggttt gaccacactc gtgacgtcaa tcaaatccgc
     2101 ggtaaccggg tgctgataag acggactgtt tcatcgacgc ctaccttaga ccgacagcgg
     2161 tcgtaagcgg cagcatgggc gacaccgcct cggtttccga acaccatgag tcgccgacgg
     2221 tcaccatcgt gccgctccat cgcagccacg cgctggtggc cgaacagcag ctcttccaat
     2281 ggttgaaacg cttcaagctg ctcatggagg tctatcacgg gctcgtgtgg cagctggctt
     2341 gtaccctcac ggtctgcttg ctggcttggc tcgccttccc cgatgtgcag ggacagtgcg
     2401 ccaacggcat agtgccagcc ctgtcctcca tcgtgcccgt gtccacgctg gctatgctac
     2461 gcggtttcgc cgagtttcgc ccacacacca ccaattttgc tcatctcacg gtggcgtgcc
     2521 tgctgatcaa caccggcatc accgtgtgca cgggcttttg cggagaaagg cgcgtcatcg
     2581 gtctgtcgtt tgccctggtg atggtctttt tcgttctctg cagcggtctc acctacctgg
     2641 ccggcaacaa tcccacgcgc tggaaagtca tcgggattgg ttacggctgg tccgtcatcg
     2701 tcttttacct attgctttac ttttcgcccg tgttgtgggt cagcaagatc tacagcggac
     2761 tctacgtcct ggtggtgacc gccgcttcgg ccgtgcttat ttacgagacc ctggatctca
     2821 tctaccagcg tggaacgttg tctaaaaaca gcgtctgtgt cagcgtcgtg ttgtacacca
     2881 tcgtcatgag tctcctaaac atgagcgtcg ccatctttag cggccacgtc tgggtgcagc
     2941 agtacgccga gaaacacggc ggacgcatcg acggcgtgag tctcctcagc ttgttataaa
     3001 aagatggggg agatgacacg ctcctgctcg cttaaacgtt ccccaccttc cctccagaga
     3061 cagacgatga acctccccca cccagtagac agacaggaca ccgcaactcg gtgttgtact
     3121 cgtgtttttt attcaacaat aaagcttttt cacgctta
//