From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: R.salmoninarum gene encoding 57 kDa extracellular protein
Date: 21 Jul 92 10:08:19 GMT
Distribution: bionet
Checksum: 47296     6

LOCUS       REN57KEPP    1811 bp ds-DNA             BCT       21-JUL-1992
DEFINITION  R.salmoninarum gene encoding 57 kDa extracellular protein
            precursor.
ACCESSION   Z12174
KEYWORDS    57 kDa extracellular protein; extracellular protein.
SOURCE      Renibacterium salmoninarum DNA.
  ORGANISM  Renibacterium salmoninarum
            Prokaryotae; Firmicutes; Regular asporogenous rods.
REFERENCE   1  (bases 1 to 1811)
  AUTHORS   Chien,M.S., O'Hara,P.J., Landolt,M.L. and Winton,J.R.
  TITLE     Sequence analysis of the 57-kilodalton soluble antigen of
            Renibacterium salmoninarum
  JOURNAL   Unpublished (1992)
  STANDARD  full staff_review
REFERENCE   2  (bases 1 to 1811)
  AUTHORS   Winton,J.R.
  JOURNAL   Unpublished (1992)
  STANDARD  full staff_review
COMMENT     *source: strain=ATCC 33209; *source: clone=clone 57kd ECP;
            From EMBL    entry RS57KEPP;  dated 09-JUN-1992.
FEATURES             Location/Qualifiers
     sig_peptide     49..126
                     /function="signal peptide"
                     /product="57kilodalton extracellular protein signal
                     peptide"
                     /citation=[1]
                     /citation=[2]
     CDS             49..1722
                     /product="57 kilodalton extracellular proteinprecursor"
                     /codon_start=1
                     /translation="MKIKKILALTAVTAVTFFGVAPLAQASQGEGNSSTSTVQGFSSV
                     NIFQQGGYSAFFHELRPDGTSSAAGGPVAAGGSYSSGDLGKKGFEDGDLVVPVVNAVF
                     GNEQKGSSFVYNKDGPAKELKVWGTTLNVGFGCNDNAPSPMGCGPANKPVASGLQPPA
                     GPLGGGTVVKVDGSNLFGASQVSFGDKPGTDIAVAQDGNSLTVKTPAVDAAGPVKVTV
                     TNPGGETVTYESFHYFGSAPTATLEPKTGPRDGGTVVKVNGGNLFDVSRVTFGDNEAT
                     EIHIAQDGNSLTVKTPAVDAAGPVEVKVSTPNGSATATDKFEYVGSDQHPDIHAPDGA
                     TPVTGKWWSGDNNTYGGWFKDGQWVLQKPNAGSVSVAFGNPGDTPVTGDWDGKGRFGI
                     GVERGGQWYLSNAGTSIGKVTAIVAFGNPGDTPVTGDWDGKGKTSIGVTRSTANGLEW
                     YITSDLTTANPPVSYHFIFGNSTDAPITGNWIGTAKTQVGLHRENSILISYDAQTVAS
                     THVFGNAGDVAVTGQWNGEGKTSFGVFEHSSGAWKLSNDLSGKIDITFN"
                     /citation=[1]
                     /citation=[2]
     mat_peptide     127..1719
                     /standard_name="major soluble antigen"
                     /product="57 kilodalton extracellular protein"
                     /citation=[1]
                     /citation=[2]
     repeat_region   496..1014
                     /rpt_type=tandem
                     /note="Two imperfect tandemrepeats"
                     /citation=[1]
                     /citation=[2]
     repeat_unit     496..756
                     /citation=[1]
                     /citation=[2]
     repeat_unit     757..1014
     repeat_region   1027..1719
                     /rpt_type=tandem
                     /note="Five imperfect tandemrepeats"
     repeat_unit     1027..1155
     repeat_unit     1156..1293
     repeat_unit     1294..1446
     repeat_unit     1447..1578
     repeat_unit     1579..1719
BASE COUNT      471 a    386 c    486 g    468 t
ORIGIN
        1 acgagcggag atggcctttc gctggtgcgc tgtggtaaaa taaaagctat gaaaataaaa
       61 aaaattttag cgctgactgc agtcactgca gtcacttttt ttggggtggc tcctcttgcg
      121 caagcttcgc aaggtgaagg gaattcttcc acttcaacag tacaaggctt cagcagtgtt
      181 aacatttttc aacagggtgg ttattctgct ttctttcacg agttaaggcc tgacgggacc
      241 tccagcgccg caggaggacc agttgcagct ggtggctctt atagttctgg tgatttaggt
      301 aaaaagggct tcgaagacgg agatcttgtt gttcccgtag taaacgcggt ttttggaaat
      361 gagcaaaaag gaagctcctt tgtttataac aaagatggtc ctgccaagga actgaaagtc
      421 tgggggacga ctctaaacgt aggtttcggg tgtaacgata atgcgccatc cccaatggga
      481 tgtggcccgg caaataaacc tgttgcatcc ggacttcagc ctccggcggg tcctcttggc
      541 ggcggtactg tggtgaaggt ggatggcagc aacctattcg gcgcatcgca agtctccttt
      601 ggagacaaac cgggaacgga cattgccgtc gctcaggatg gtaactcgtt gactgtaaaa
      661 acaccagcag tagatgccgc tggcccggta aaggttacag tcactaaccc cggtggggag
      721 acggtaacgt acgagagttt ccactacttc ggaagcgccc cgacagcaac tctcgagcct
      781 aagactgggc ctcgtgacgg cggtactgtg gtgaaggtga atggcggcaa cctattcgac
      841 gtatcgagag ttaccttcgg agacaacgag gcaacggaaa ttcacatcgc gcaggatggc
      901 aactcgttga ctgtaaaaac accagcagta gatgccgctg gcccggtaga ggttaaagtc
      961 tctacaccta acggttccgc aacagcaact gacaaatttg aatacgtggg tagtgaccaa
     1021 cacccagata tccatgcacc agatggagca actccggtta ctggtaaatg gtggtctggc
     1081 gacaacaaca cgtatggtgg atggttcaag gacggccaat gggtactgca aaagccaaat
     1141 gctggttccg tttcagttgc ttttggtaat cccggtgata ctcctgtcac aggtgactgg
     1201 gatggtaaag gtcgatttgg tattggcgtt gagagaggcg gccaatggta tctatcgaat
     1261 gctggaactt ctattggtaa agtaacagcg atcgttgctt ttggtaatcc cggtgatact
     1321 cctgtcacag gtgactggga tggtaaaggt aaaacatcga ttggtgtgac acggtctact
     1381 gctaatggat tagagtggta tattacatca gatctaacca ctgccaatcc gcctgtttcc
     1441 tatcatttca tattcggaaa ttctactgat gccccaatta ctgggaactg gatcgggact
     1501 gcaaaaaccc aggttgggtt acatcgtgag aatagtattt tgattagtta cgatgcccag
     1561 actgttgcct caacccatgt ctttggtaat gctggggatg ttgcggtaac tggtcagtgg
     1621 aatggtgagg gtaagacgag tttcggtgtt tttgagcact cctccggagc ttggaaacta
     1681 tctaatgacc ttagcggaaa aatagatatt acctttaact aaggtttatt aaatccttaa
     1741 atgaaaaaag cccatccact ggatgggctt ttttcattta aggatttaat aaaccttagt
     1801 taaaggttgt t
//