From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Mus musculus myogenin gene, complete cds.
Date: 4 Sep 92 10:05:01 GMT
Distribution: bionet
Checksum: 10535     9

LOCUS       MUSMYOGEN    4145 bp ds-DNA             ROD       04-SEP-1992
DEFINITION  Mus musculus myogenin gene, complete cds.
ACCESSION   M95800
KEYWORDS    cell conversion to myoblast; muscle structural gene transactivator;
            myogenic regulatory factor; myogenin; nuclear protein;
            positive regulation.
SOURCE      Mus musculus (strain BALB/c, sub_species domesticus) (library:
            EMBL3-Clontech) liver DNA; and Mus musculus (strain DBA/2J)
            (library: EMBL3-Clontech) liver DNA.
  ORGANISM  Mus musculus
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Rodentia; Myomorpha; Muridae; Murinae.
REFERENCE   1  (sites)
  AUTHORS   Edmondson,D.G. and Olson,E.N.
  TITLE     A gene with homology to the myc similarity region of MyoD1 is
            expressed during myogenesis and is sufficient to activate the
            muscle differentiation program
  JOURNAL   Genes Dev. 3, 628-640 (1989)
  STANDARD  full staff_review
REFERENCE   2  (sites)
  AUTHORS   Fujisawa-Sehara,A., Hosoda,Y., Obinata,T. and Nabeshima,Y.
  TITLE     Myogenin contains two domains conserved among myogenic factors
  JOURNAL   J. Biol. Chem. 265, 15219-15223 (1990)
  STANDARD  full staff_review
REFERENCE   3  (bases 1 to 4145)
  AUTHORS   Edmondson,D.G., Cheng,T.-C., Cserjesi,P., Chakraborty,T. and
            Olson,E.N.
  TITLE     Analysis of the myogenin promoter reveals an indirect pathway for
            positive autoregulation mediated by the muscle-specific enhancer
            factor MEF-2
  JOURNAL   Mol. Cell. Biol. 12, 3665-3677 (1992)
  STANDARD  simple staff_entry
FEATURES             Location/Qualifiers
     mRNA            join(1570..2089,2604..2685,3213..4015)
                     /gene="myogenin"
     CDS             join(1619..2089,2604..2685,3213..3334)
                     /function="can convert other cell types to myoblasts"
                     /function="can transactivate muscle structural genes"
                     /product="myogenin"
                     /gene="myogenin"
                     /codon_start=1
                     /translation="MELYETSPYFYQEPHFYDGENYLPVHLQGFEPPGYERTELSLSP
                     EARGPLEEKGLGTPEHCPGQCLPWACKVCKRKSVSVDRRRAATLREKRRLKKVNEAFE
                     ALKRSTLLNPNQRLPKVEILRSAIQYIERLQALLSSLNQEERDLRYRGGGGPQPMVPS
                     ECNSHSASCSPEWGNALEFGPNPGDHLLAADPTDAHNLHSLTSIVDSITVEDMSVAFP
                     DETMPN"
     misc_binding    complement(1497..1511)
                     /bound_moiety="muscle-specific enhancer factor 2 (MEF-2)"
                     /gene="myogenin"
     misc_binding    1516..1529
                     /bound_moiety="NF-1"
                     /gene="myogenin"
     TATA_signal     1540..1545
                     /gene="myogenin"
     misc_binding    1555..1560
                     /bound_moiety="myogenic regulatory factors"
                     /note="E-1 box"
                     /gene="myogenin"
                     /evidence=EXPERIMENTAL
     variation       replace(1562,"t")
                     /note="in strain DBA/2J"
                     /gene="myogenin"
     exon            1570..2089
                     /number=1
                     /gene="myogenin"
                     /evidence=EXPERIMENTAL
     intron          2090..2603
                     /number=1
                     /gene="myogenin"
     exon            2604..2685
                     /number=2
                     /gene="myogenin"
                     /evidence=EXPERIMENTAL
     intron          2686..3212
                     /number=2
                     /gene="myogenin"
     exon            3213..4015
                     /number=3
                     /gene="myogenin"
                     /evidence=EXPERIMENTAL
BASE COUNT     1051 a   1055 c   1129 g    908 t      2 others
ORIGIN      chromosome 1
        1 tctagagttg tatgacgcag gcaaaggtga tgactcaggc aggaaggaat agaagaggcc
       61 agcctggtgg cccaggacag acaaatgatg caaaggactc tttctcctta tcgacccttc
      121 tacagaaagg aaagagtcaa aacggttcta gtgccagaag gcattattga ggggaaagca
      181 cagaagagat gattaagagc atcagacagg gtccatccca taatcagcca ccaaacacag
      241 acactattac atatgccagc aagattttgc tgaaagaacc ctgatatagc tgcctcttgt
      301 gaggctatgc agtgcctggc aaatacagaa gtggatgctc acagtcagct attggatgga
      361 acacagggcc cccaatggag gagctagaga aagtacccaa ggaactgaag gggtctgcaa
      421 ccctataggt ggaacaacaa tatgaactaa ccagtacccc cagagctcat gtctctagct
      481 gcatatgtag cagaagatgg cctagtcggc catcattggg aagagaggcc ccttggtatt
      541 gcaaactata tgccccagta caggggaacg ccagggccaa gaagtgggaa tgagtgggta
      601 ggggagcagg gcggggggag gggggggtat agggaacttt tgggatagca tttgaaatgt
      661 aaatgaagaa aatatctaat aaaaaataat ttaaaaaaga gcgtcagaca ggggactgaa
      721 cagctcttgc actaggggag aagaaggcaa tgtagagtag tctgtgagtt ctaatccttg
      781 ctaaacactg acttcacctg acccctacta cttaaggccc ccccccctta cttaagaagt
      841 ccctgtgttc tcttacttca atctaccccc aacatcatga gacctggtca aagaagctgt
      901 agaaacccaa aagttgaatc catttgccct tctgggtttc tgtctttgcc tccatggacg
      961 ataggacaca cacacacaca cacacacaca cacacacaca cacacacaca cgccccaaat
     1021 ctggagtggt cctgatgtgg tagtggtagg tctttagggg tctcatggga ctgacatagt
     1081 acggtttaag gtgctgctgc tgagcaggaa agagaaggct aagtggattt tcaagacccc
     1141 ttcccgtccg tccaagacaa cccctttctt gttcccttcc tgccctgtcc accagctgcc
     1201 ttggaccatg gaggagagag taggcaggag gcccgggtag gagtaattga aaggagcaga
     1261 tgagacgggg gaatgcaccc acccccacct tccctgcccc acaggntgtg gagaaatgaa
     1321 aactaatcaa attacagccg acggcctccc gacccgtgca caggagccgc ctgggccagg
     1381 ggcaggcctg cagggtgggg tgggggcaaa aggagaggga aggggaatca catgtaatcc
     1441 actggaaacg tcttgatgtg cagcaacagc ttagaggggg gctcaggttt ctgtggcgtt
     1501 ggctatattt atctctgggt tcatgccagc agggagggtt taaatggcac ccagcagttg
     1561 gcgtgagggg ctgcgggagc ttgggggcca gtggcaggaa caagcctttt ccgacctgat
     1621 ggagctgtat gagacatccc cctatttcta ccaggagccc cacttctatg atggggaaaa
     1681 ctaccttcct gtccaccttc agggcttcga gcccccgggc tatgagcgga ctgagctcag
     1741 cttaagcccg gaagcccgag ggcccctgga agaaaaggga ctggggaccc ctgagcattg
     1801 tccaggccag tgcctgccgt gggcatgtaa ggtgtgtaag aggaagtctg tgtcggtgga
     1861 ccggaggagg gcagccacac tgagggagaa gcgcaggctc aagaaagtga atgaggcctt
     1921 cgaggccctg aagaggagca ccctgctcaa ccccaaccag cggctgccta aagtggagat
     1981 cctgcgcagc gccatccagt acattgagcg cctacaggcc ttgctcagct ccctcaacca
     2041 ggaggagcgc gatctccgct acagaggcgg gggcgggccc cagcccatgg taagtggcta
     2101 gtacaccaga tccagggata gggccagagg gaggtgcctg gtcagccccg ggccaccaga
     2161 gctagaacag gtgcaaagca gggcccttgg gatccctgga cccctttcct tgtcggctag
     2221 actgtccagt ggccttctgg gctcatactg tttccaaaat acagatgggg tagggtggac
     2281 tggcacagga gaacagaagg ggcccggaaa caagcctgca gtaagggctt tggtgagcct
     2341 aggtgaaaat gagggaaaag tggcctaaga aacctgcttt gtccaagtcc acagccttca
     2401 tttttgacct gacgaaacac attagaatgc ccacaggcct gctcaggagc caccgtaaca
     2461 aaatactgtg gtttatcacc ctcaccgccg aaatgaaagc aaagttctca tcctagaagc
     2521 taggaaagac ctagaagtag gatgtctatg gtaacattct gcagtagact tgaccttgga
     2581 ccttggcctt gtcttgcatg caggtgccca gtgaatgcaa ctcccacagc gcctcctgca
     2641 gtccggagtg gggcaatgca ctggagttcg gtcccaaccc aggaggtaag tgaatcctga
     2701 ccctggtaac atggctcaaa agggtaggca actgaccctg taacccaggc agagagaaat
     2761 acctgagact gtcccttagt ccctacctag gctgggcttg tacccttacc ataagccctg
     2821 tcccaatcac ccttcagcag cttttctagg aaattgctga cctgagggcc ccaaatggtg
     2881 ggctggagac cctgtgcctc ccggtagcct tgaccagcta aagagtagtc agagcctcca
     2941 gcaacttccc tgctggtctc tgacctcaga gagaagagcc tgctcctgcc ccaggggtcc
     3001 ctggggatca ctcagtcagt gttgtaaaac tgtgccctgt gccctaggat atcctggctt
     3061 cacctaggga tagccacttt taaaacccca caagcacaca cacagcatgg ggagtactag
     3121 tgtcttgggg gcggggaaca ctcctgagtc ttccttaccc attttgtcct ggaccagttg
     3181 cccaggaaac attctcccac tttttcttgc agatcatttg ctcgcggctg accctacaga
     3241 cgcccacaat ctgcactccc ttacgtccat cgtggacagc atcacggtgg aggatatgtc
     3301 tgttgccttc ccagacgaaa ccatgcccaa ctgagattgt ctgtcaggct gggtgtgcat
     3361 gtgagccccc aagttggtgt caaaagccat cacttctgta gcagggggct tttaagtggg
     3421 gctgtcctga tgtccagaaa acagccctgg gctgccacaa gccagactcc ccactcccca
     3481 ttcacataag gctaacaccc agcccagcga gggaatttag ctgactcctt aaagcagaga
     3541 gcatcctctt ctgaggagag aaagatggag tccagagagc ccccttgtta atgtccctca
     3601 gtggggcaaa ctcaggagct tcttttttgt ttatcataat atgcctcgaa ttccaccctc
     3661 cacctccaaa atgaaaccgt ttgagagaca tgagtgccct gacctggaca agtgtgcaca
     3721 tctgttctag tctcttcctg aagccagtgg ctgggctggg cctgccctga gttgagagag
     3781 aagggggagg agctatccgg ttccaaagcc tctgggggcc aagcatttgc agtggatctt
     3841 gggaaccttc cagtgctttg tgtattgttt attgttttgt gtgttgtttg taaagctgcc
     3901 gtctgccaag gtctcctgtg ctgatgatac cgggaacagg caggcaaggg ggtgggggct
     3961 cttggggtga cttcttttgt taactaagca ttgtgtggtt ttgccaattt tttttctttt
     4021 gtaattcttt tgctaactta tttggatttc cttttttaaa aaatgaataa agactggttg
     4081 ctatcagagc atctgcaatg atattatcaa ganggggagc caccaaaact cttgacacct
     4141 ttacc
//