From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Transposon Tn7 aadA gene encoding streptomycin adenylyltransferase,
Date: 27 Jan 93 06:02:38 GMT
Distribution: bionet
Checksum: 22023 6

LOCUS       TRN7AADA     1614 bp ds-DNA             BCT       26-JAN-1993
DEFINITION  Transposon Tn7 aadA gene encoding streptomycin adenylyltransferase,
            complete cds.
ACCESSION   X03043
KEYWORDS    O-nucleotidyltransferase; adenylyl transferase;
            antibiotic resistance; drug resistance gene;
            spectinomycin resistance; streptomycin resistance; transferase;
            transposon.
SOURCE      Transposon Tn7 DNA, clone pFE506.
  ORGANISM  Transposon Tn7
            Prokaryotae.
REFERENCE   1  (bases 1 to 1614)
  AUTHORS   Fling,M.E., Kopf,J. and Richards,C.
  TITLE     Nucleotide sequence of the transposon Tn7 gene encoding an
            aminoglycoside-modifying enzyme, 3'(9)-O-nucleotidyltransferase
  JOURNAL   Nucleic Acids Res. 13, 7095-7106 (1985)
  STANDARD  full automatic
COMMENT     EMBL features not translated to GenBank features:
              key        from     to       description
              RPT          21     65       direct repeat
              INVREP       22     47       inverted repeat A
              PRM          40     43       pot. -35 region
              INVREP       51     65       inverted repeat A
              SITE         22     65       pot. stem loop structure
              PRM          62     67       pot. -10 region
              RBS          69     72       pot. rRNA binding site
              RBS          84     88       pot. altern. rRNA-binding site
              SITE         94     94       pot. alternate translation start
              RPT         874    928       direct repeat
              INVREP      875    900       inverted repeat B
              INVREP      904    928       inverted repeat B'
              SITE        875    928       pot. stem loop structure.
FEATURES             Location/Qualifiers
     repeat_region   21..75
                     /rpt_type=DIRECT
     stem_loop       22..75
                     /note="putative"
     repeat_region   22..47
                     /rpt_type=INVERTED
     -35_signal      40..43
     repeat_region   51..75
                     /rpt_type=INVERTED
     -10_signal      62..67
     RBS             69..72
                     /note="rRNA binding site; putative"
     RBS             84..88
                     /note="alternative rRNA binding site; putative"
     repeat_region   875..900
                     /rpt_type=INVERTED
                     /note="875"
     repeat_region   904..928
                     /rpt_type=INVERTED
     CDS             82..870
                     /note="3';  (9)-O-nucleotidyltransferase"
                     /codon_start=1
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
BASE COUNT      419 a    406 c    416 g    373 t
ORIGIN      At AvaI site; about 1.0 kb downstream of dhfr gene.
        1 ccgggagcac aggatgacgc ctaacaattc attcaagccg acaccgcttc gcggcgcggc
       61 ttaattcagg agttaaacat catgagggaa gcggtgatcg ccgaagtatc gactcaacta
      121 tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc cgtacatttg
      181 tacggctccg cagtggatgg cggcctgaag ccacacagtg atattgattt gctggttacg
      241 gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga tcaacgacct tttggaaact
      301 tcggcttccc ctggagagag cgagattctc cgcgctgtag aagtcaccat tgttgtgcac
      361 gacgacatca ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg agaatggcag
      421 cgcaatgaca ttcttgcagg tatcttcgag ccagccacga tcgacattga tctggctatc
      481 ttgctgacaa aagcaagaga acatagcgtt gccttggtag gtccagcggc ggaggaactc
      541 tttgatccgg ttcctgaaca ggatctattt gaggcgctaa atgaaacctt aacgctatgg
      601 aactcgccgc ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt
      661 tggtacagcg cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg
      721 gagcgcctgc cggcccagta tcagcccgtc atacttgaag ctaggcaggc ttatcttgga
      781 caagaagatc gcttggcctc gcgcgcagat cagttggaag aatttgttca ctacgtgaaa
      841 ggcgagatca ccaaggtagt cggcaaataa tgtctaacaa ttcgttcaag ccgacgccgc
      901 ttcgcggcgc ggcttaactc aagcgttaga gagctgggga agactatgcg cgatctgttg
      961 aaggtggttc taagcctcgt acttgcgatg gcatcggggc aggcacttgc tgacctgcca
     1021 attgttttag tggatgaagc tcgtcttccc tatgactact ccccatccaa ctacgacatt
     1081 tctccaagca actacgacaa ctccataagc aattacgaca atagtccatc aaattacgac
     1141 aactctgaga gcaactacga taatagttca tccaattacg acaatagtcg caacggaaat
     1201 cgtaggctta tatatagcgc aaatgggtct cgcactttcg ccggctacta cgtcattgcc
     1261 aacaatggga caacgaactt cttttccaca tctggcaaaa ggatgttcta caccccaaaa
     1321 ggggggcgcg gcgtctatgg cggcaaagat gggagcttct gcggggcatt ggtcgtcata
     1381 aatggccaat tttcgcttgc cctgacagat aacggcctga agatcatgta tctaagcaac
     1441 tagcctgctc tctaataaaa tgttaggcct caacatctag tcgcaagctg aggggaacca
     1501 ctagtgtcat acgaacctcc aagagacggt tacacaaacg ggtacattgt tgatgtcatg
     1561 tatgacaatc gcccaagtaa gtatccagct gtgttcagaa cgtacgtccg aatt
//