From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: S.cerevisiae dihydrolipoamide dehydrogenase mRNA, complete cds.
Date: 29 Dec 92 21:28:51 GMT
Distribution: bionet
Checksum: 09589 5

LOCUS       YSCDHDHA     2108 bp ss-mRNA            PLN       14-DEC-1992
DEFINITION  S.cerevisiae dihydrolipoamide dehydrogenase mRNA, complete cds.
ACCESSION   J03645
KEYWORDS    dihydrolipoamide dehydrogenase.
SOURCE      Saccharomyces cerevisiae (strain 20B-12) cDNA to mRNA.
  ORGANISM  Saccharomyces cerevisiae
            Eukaryota; Fungi; Eumycota; Hemiascomycetes; Endomycetales;
            Saccharomycetaceae.
REFERENCE   1  (bases 1 to 2108)
  AUTHORS   Browning,K.S., Uhlinger,D.J. and Reed,L.J.
  TITLE     Nucleotide sequence for yeast dihydrolipoamide dehydrogenase
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 85, 1831-1834 (1988)
  STANDARD  full automatic
COMMENT     Draft entry and computer-readable sequence for [1] kindly provided
            by K.S.Browning, 29-MAR-1988.
FEATURES             Location/Qualifiers
     sig_peptide     532..594
                     /gene="dihydrolipoamide dehydrogenase"
                     /codon_start=1
     mat_peptide     595..2028
                     /product="dihydrolipoamide dehydrogenase"
                     /gene="dihydrolipoamide dehydrogenase"
                     /codon_start=1
     CDS             532..2031
                     /EC_number="1.8.1.4"
                     /gene="dihydrolipoamide dehydrogenase"
                     /product="dihydrolipoamide dehydrogenase"
                     /codon_start=1
                     /translation="MLRIRSLLNNKRAFSSTVRTLTINKSHDVVIIGGGPAGYVAAIK
                     AAQLGFNTACVEKRGKLGGTCLNVGCIPSKALLNNSHLFHQMHTEAQKRGIDVNGDIK
                     INVANFQKAKDDAVKQLTGGIELLFKKNKVTYYKGNGSFEDETKIRVTPVDGLEGTVK
                     EDHILDVKNIIVATGSEVTPFPGIEIDEEKIVSSTGALSLKEIPKRLTIIGGGIIGLE
                     MGSVYSRLGSKVTVVEFQPQIGASMDGEVAKATQKFLKKQGLDFKLSTKVISAKRNDD
                     KNVVEIVVEDTKTNKQENLEAEVLLVAVGRRPYIAGLGAEKIGLEVDKRGRLVIDDQF
                     NSKFPHIKVVGDVTFGPMLAHKAEEEGIAAVEMLKTGHGHVNYNNIPSVMYSHPEVAW
                     VGKTEEQLKEAGIDYKIGKFPFAANSRAKTNQDTEGFVKILIDSKTERILGAHIIGPN
                     AGEMIAEAGLALEYGASAEDVARVCHAHPTLSEAFKEANMAAYDKAIHC"
BASE COUNT      675 a    396 c    468 g    569 t
ORIGIN      192 bp upstream of BglII site.
        1 gggaattcgc tggtatccaa attgttgctg atgacgtgac ggtcaccaac ccagctagaa
       61 ttgctaccgc catcgaagag tgtctgaatc catcaaggct gctcaagact ctttcgctgc
      121 gtaccttgtc tgaatccatc aaggctgctc aagactcttt cgctgccaac tggggtgtta
      181 tggtttccca cagatctggt gaaactgaag acactttcat tgctgacttg gttgtcggtt
      241 tgagaactgg tcaaatcaag actggtgctc cagctagatc cgaaagattg gctaagttga
      301 accaattgtt gagaatcgaa gaagaattgg gtgacaaggc tgtctacgcc ggtgaaaact
      361 tccaccacgg tgacaagttg taaagtgctt ttaactaaga attattagtc ttttctgctt
      421 attttttcat catagtttag aacactttat attaacgaat agtttatgaa tctatttagg
      481 tttaaaaatt gatacagttt tataaaaaaa aaaaaaaaaa aaaattccac aatgttaaga
      541 atcagatcac tcctaaataa taagcgtgcc ttttcgtcca cagtcaggac attgaccatt
      601 aacaagtcac atgatgtagt catcatcggt ggtggccctg ctggttacgt ggctgctatc
      661 aaagctgctc aattgggatt taacactgca tgtgtagaaa aaagaggcaa attaggcggt
      721 acctgtctta acgttggatg tatcccctcc aaagcacttc taaataattc tcatttattc
      781 caccaaatgc atacggaagc gcaaaagaga ggaattgacg tcaacggtga tatcaaaatt
      841 aacgtagcaa acttccaaaa ggctaaggat gacgctgtta agcaattaac tggaggtatt
      901 gagcttctgt tcaagaaaaa taaggtcacc tattataaag gtaatggttc attcgaagac
      961 gaaacgaaga tcagagtaac tcccgttgat gggttggaag gcactgtcaa ggaagaccac
     1021 atactagatg ttaagaacat catagtcgcc acgggctctg aagttacacc cttccccggt
     1081 attgaaatag atgaggaaaa aattgtctct tcaacaggtg ctctttcgtt aaaggaaatt
     1141 cccaaaagat taaccatcat tggtggagga atcatcggat tggaaatggg ttcagtttac
     1201 tctagattag gctccaaggt tactgtagta gaatttcaac ctcaaattgg tgcatctatg
     1261 gacggcgagg ttgccaaagc cacccaaaag ttcttgaaaa agcaaggttt ggacttcaaa
     1321 ttaagcacca aagttatttc tgcaaagaga aacgacgaca agaacgtcgt cgaaattgtt
     1381 gtagaagata ctaaaacgaa taagcaagaa aatttggaag ctgaagtttt gctggttgct
     1441 gttggtagaa gaccttacat tgctggctta ggggctgaaa agattggatt agaagtagac
     1501 aaaaggggac gcctagtcat tgatgaccaa tttaattcca agttcccaca cattaaagtg
     1561 gtaggagatg ttacatttgg tccaatgctg gctcacaaag ccgaagagga aggtattgca
     1621 gctgtcgaaa tgttgaaaac tggtcacggt catgtcaact ataacaacat tccttcggtc
     1681 atgtattctc acccagaagt agcatgggtt ggtaaaaccg aagagcaatt gaaagaagcc
     1741 ggcattgact ataaaattgg taagttcccc tttgcggcca attcaagagc caagaccaac
     1801 caagacactg aaggtttcgt gaagattttg atcgattcca agaccgagcg tattttgggg
     1861 gctcacatta tcggtccaaa tgccggtgaa atgattgctg aagctggctt agccttagaa
     1921 tatggcgctt ccgcagaaga tgttgctagg gtctgccatg ctcatcctac tttgtccgaa
     1981 gcatttaagg aagctaacat ggctgcctat gataaagcta ttcattgttg aaaacaggaa
     2041 ataataaaca gtatagtata tatatttatg aagaaccgct tagtattgag taaaaaaaaa
     2101 aggaattc
//