From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Leishmania tarentolae kinetoplast pre-edited mitochondrial
Date: 29 Dec 92 00:45:47 GMT
Distribution: bionet
Checksum: 23884 48

LOCUS       LEIKPMAX    20991 bp ds-DNA             ORG       03-DEC-1992
DEFINITION  Leishmania tarentolae kinetoplast pre-edited mitochondrial
            maxicircle DNA complete transcribed region and flanks.
ACCESSION   M10126 J02707 M10127 M11022 N00030
KEYWORDS    12S ribosomal RNA; 9S ribosomal RNA; NADH dehydrogenase;
            cytochrome; cytochrome oxidase; oxidase; ribosomal RNA.
SOURCE      Leishmania tarentolae kinetoplast DNA.
  ORGANISM  Kinetoplast Leishmania tarentolae
            Eukaryota; Animalia; Protozoa; Sarcomastigophora; Mastigophora;
            Kinetoplastida; Trypanosomatina; Trypanosomatidae.
REFERENCE   1  (bases 2213 to 6561; 9425 to 16392)
  AUTHORS   De la Cruz,V.F., Neckelmann,N. and Simpson,L.
  TITLE     Sequences of six genes and several open reading frames in the
            kinetoplast maxicircle DNA of Leishmania tarentolae
  JOURNAL   J. Biol. Chem. 259, 15136-15147 (1984)
  STANDARD  full automatic
REFERENCE   2  (bases 1 to 1665)
  AUTHORS   De la Cruz,V.F., Simpson,A.M., Lake,J.A. and Simpson,L.
  TITLE     Primary sequence and partial secondary structure of the 12S
            kinetoplast (mitochondrial) ribosomal RNA from Leishmania
            tarentolae: Conservation of peptidyl-transferase structural
            elements
  JOURNAL   Nucleic Acids Res. 13, 2337-2356 (1985)
  STANDARD  full automatic
REFERENCE   3  (bases 18233 to 20991)
  AUTHORS   Muhich,M.L., Neckelmann,N. and Simpson,L.
  TITLE     The divergent region of the Leishmania tarentolae kinetoplast
            maxicircle DNA contains a diverse set of repetitive sequences
  JOURNAL   Nucleic Acids Res. 13, 3241-3260 (1985)
  STANDARD  full automatic
REFERENCE   4  (sites)
  AUTHORS   Simpson,A.G., Necklemann,N., De la Cruz,V.F., Muhich,M.L. and
            Simpson,L.
  TITLE     Mapping and 5' end determination of kinetoplast maxicircle gene
            transcripts from Leishmania tarentolae
  JOURNAL   Nucleic Acids Res. 13, 5977-5993 (1985)
  STANDARD  full automatic
REFERENCE   5  (bases 1582 to 2415)
  AUTHORS   De la Cruz,V.F., Lake,J.A., Simpson,A.M. and Simpson,L.
  TITLE     A minimal ribosomal RNA: Sequence and secondary structure of the 9S
            kinetoplast ribosomal RNA from Leishmania tarentolae
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 82, 1401-1405 (1985)
  STANDARD  full automatic
REFERENCE   6  (bases 6481 to 9501; 16391 to 18232)
  AUTHORS   Simpson,L., Neckelmann,N., De la Cruz,V.F., Simpson,A.M.,
            Feagin,J.E., Jasmer,D.P. and Stuart,K.D.
  TITLE     Comparison of the maxicircle (mitochondrial) genomes of Leishmania
            tarentolae and Trypanosoma brucei at the level of nucleotide
            sequence
  JOURNAL   J. Biol. Chem. 262, 6182-6196 (1987)
  STANDARD  full automatic
REFERENCE   7  (sites)
  AUTHORS   Shaw,J., Campbell,D. and Simpson,L.
  TITLE     Internal frameshifts within the mitochondrial genes for cytochrome
            oxidase subunit II and maxicircle unidentified reading frame 3 of
            Leishmania tarentolae are corrected by RNA editing: evidence for
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 86, 6220-6224 (1989)
  STANDARD  full automatic
REFERENCE   8  (bases 1 to 20991)
  AUTHORS   Simpson,L.
  JOURNAL   Unpublished (1991)
  STANDARD  full automatic
COMMENT     [4]  sites; ->mRNA.
               Draft entry and sequence were kindly provided in
            computer-readable form by L.Simpson, 03-JUN-1986.  Reprint for [6]
            kindly provided by N.Neckelmann, 22-SEP-1986.
               The initiation point of cytochrome oxidase subunit III, and
            MURF[1-3] are annotated as undetermined because the probable start
            codon has not been identified.
               The 'pre-edited regions' noted as misc_features are regions of
            the mRNAs to which uridines are added (or deleted) by RNA editing.
            'MURFs' are maxicircle unidentified reading frames.
               Guide RNAs or gRNAs possess 3' oligo-[U] tails of heterogeneous
            length (5-28 nt) added post-transcriptionally by the mitochondrial
            terminal uridylyl transferase (reference 4 below). The gRNAs appear
            to be primary transcripts since the 5' ends have di- or
            tri-phosphates. 3'-truncated gRNAs exist also at a low frequency
            (reference 4 below).
               Note that the localization of these genes in the maxicircle
            genome have no relationship to the cryptogenes for which they
            mediate editing of the preedited transcripts. 'Cryptogene' is a
            term for those genes whose transcripts are edited to produce
            translatable mRNAs.
               The extent of editing varies from a few uridines added at a few
            sites, to 30-40 uridines added at 10-20 sites, to hundreds of
            uridines added over the entire length of the RNA (references 6, 7,
            8, 3 below). This latter type of editing is termed 'pan-editing'
            and requires multiple overlapping gRNAs for the entire region to be
            edited properly (reference 2 below). Editing proceeds 3' to 5',
            both within a single editing 'block' (= a single gRNA) or within an
            editing domain. (reference 9 below). The RPS12 and ND7 genes have
            several editing domains, which appear to be edited independently,
            3' to 5' (reference 3) In some cases editing creates AUG methionine
            translation initiation codons, and TAA termination codons.
            Partially edited RNAs, edited only in the 3' region of a domain,
            are found in steady state RNA (references 9, 3, 1).
               References which were not in the previous entry:
               1.     Sturm, N.R.,  Maslov, D.A.,  Blum, B.  and Simpson,  L.
                'Generation   of   unexpected   editing   patterns   in
                Leishmania tarentolae  mitochondrial mRNAs:  Misediting
                produced by misguiding.' Cell 70, 469-476 (1992).
               2.   Maslov, D.A.  and Simpson, L., 'The polarity of editing
                within  a multiple  gRNA-mediated  domain  is  due  to
                formation of  anchors for upstream gRNAs by downstream
                editing.' Cell 70, 459-467 (1992).
               3.     Maslov, D.A., Sturm, N.R., Niner, B.M., Gruszynski,E.S.
                and  Simpson,  L.,  'An  intergenic  G-rich  region  in
                Leishmania tarentolae  kinetoplast maxicircle  DNA is a
                pan-edited cryptogene  encoding ribosomal protein S12.'
                Mol. Cell. Biol. 12, 56-67 (1991).

            4.    Blum, B.  and Simpson, L., 'Guide RNAs in kinetoplastid
                mitochondria  have   a  non-encoded  3'  oligo(U)  tail
                involved in  recognition of  the preedited region. Cell
                62, 391-397 (1990).

            5.    Blum, B.,  Bakalara, N. and Simpson,  L., 'A model for
                RNA editing  in kinetoplastid mitochondria: 'Guide RNA'
                molecules transcribed  from maxicircle  DNA provide the
                edited information.' Cell. 60, 189-198 (1990).

               6. Feagin, J., Shaw, J., Simpson, L. and Stuart, K., 'Creation
            of AUG initiation codons by addition of uridine within cytochrome b
            transcripts of three kinetoplastid species.' Proc. Natl. Acad. Sci.
            USA. 85, 539-543 (1988).
               7. Shaw, J.,  Feagin, J.,  Stuart, K. and Simpson, L., 'Editing

                of  kinetoplastid  mitochondrial mRNAs by   uridine
                addition and  deletion generates  conserved amino acid
                sequences and AUG initiation codons.' Cell. 53, 401-411
                (1988).
               8.  Shaw,  J.,  Campbell,  D.  and  Simpson,  L.,   'Internal
                frameshifts  within   the  mitochondrial  genes  for
                cytochrome   oxidase    subunit   II   and   maxicircle
                unidentified reading  frame 3  of Leishmania tarentolae
                are corrected  by RNA editing: Evidence for translation
                of the  edited cytochrome oxidase  subunit  II  mRNA.'
                Proc. Natl. Acad. Sci. USA.  86, 6220-6224 (1989).
               9. Sturm, N. and Simpson, L., 'Partially edited mRNAs for
            cytochrome b and subunit III of cytochrome oxidase from Leishmania
            tarentolae mitochondria: RNA editing intermediates.' Cell 61,
            871-878 (1990).
FEATURES             Location/Qualifiers
     misc_feature    6482..7123
                     /note="This feature applies to a gene which lacks a coding
                     region feature. "
                     /gene="MURF4"
     mRNA            complement(<11181..12865)
                     /product="cytochrome oxidase subunit I"
     misc_RNA        complement(102..150)
                     /note="guide RNA (gM150) This gRNA was discovered as part
                     of a mis-editing gRNA/mRNA chimeric molecule and the
                     precise editing role is unknown"
     misc_RNA        complement(347..395)
                     /note="guide RNA (gND7-II)"
     rRNA            438..1610
                     /product="12S ribosomal RNA"
     rRNA            1639..2249
                     /product="9S ribosomal RNA"
     misc_RNA        2239..2291
                     /note="guide RNA (gCYb-II)"
     mRNA            (3261.3265)..>4458
                     /standard_name="MURF3"
                     /citation=[6]
                     /note="major alternate"
     misc_feature    3315..3325
                     /standard_name="MURF3"
                     /citation=[8]
                     /note="pre-edited region"
     CDS             3326..4458
                     /gene="ND7"
                     /codon_start=1
                     /citation=[8]
                     /note="Unedited version of the ND7.; putative"
     mRNA            (3351.3355)..>4458
                     /standard_name="MURF3"
                     /citation=[6]
                     /note="minor alternate"
                     /gene="ND7"
     mRNA            4466..>5362
                     /product="cytochrome oxidase subunit III"
     misc_feature    4511..4553
                     /product="cytochrome oxidase subunit III"
                     /citation=[8]
                     /note="pre-edited region"
     CDS             4553..5362
                     /product="cytochrome oxidase subunit III"
                     /codon_start=1
                     /citation=[8]
                     /note="Unedited version of COIII"
     mRNA            5329..>6481
                     /product="cytochrome b"
                     /note="major alternate"
     mRNA            (5382.5386)..>6481
                     /product="cytochrome b"
                     /citation=[6]
                     /note="minor alternate"
     misc_feature    5403..5425
                     /product="cytochrome b"
                     /citation=[8]
                     /note="pre-edited region"
     CDS             6482..7123
                     /gene="MURF4"
                     /codon_start=1
                     /citation=[8]
                     /note="Unedited version of MURF4; putative"
     misc_feature    6529..6606
                     /standard_name="MURF4"
                     /citation=[8]
                     /note="possible pre-edited region"
     mRNA            9459..>10124
                     /product="cytochrome oxidase subunit II"
     CDS             9496..10124
                     /product="cytochrome oxidase subunit II"
                     /gene="COII"
                     /codon_start=1
                     /citation=[8]
                     /note="Unedited version of COII"
     misc_RNA        9893..9908
                     /note="guide RNA (gMURF2-I)"
                     /gene="COII"
     mRNA            (10107.10113)..>11190
                     /standard_name="MURF2"
                     /citation=[6]
                     /note="major alternate"
     misc_RNA        complement(10117..10137)
                     /note="guide RNA (gCOII); this gRNA edits in cis. It is
                     located at the 3' end of the COII mRNA and folds back to
                     edit the mRNA)"
     misc_feature    10146..10169
                     /standard_name="MURF2"
                     /citation=[8]
                     /note="pre-edited region"
     CDS             10169..11190
                     /gene="MURF2"
                     /codon_start=1
                     /citation=[8]
                     /partial
                     /note="Unedited version of the MURF2"
     mRNA            10434..>11190
                     /standard_name="MURF2"
                     /citation=[6]
                     /note="minor alternate"
                     /gene="MURF2"
     misc_feature    10644..10832
                     /note="conserved autonomous replicating sequence"
     misc_RNA        complement(13090..13144)
                     /note="guide RNA (gMURF2-II)"
     mRNA            (13117.13121)..>14465
                     /product="NADH dehydrogenase subunit 4"
                     /citation=[6]
     mRNA            14898..>16696
                     /product="NADH dehydrogenase subunit 5"
     repeat_region   16700..20991
                     /rpt_type=tandem
                     /citation=[3]
                     /note="divergent region"
     misc_RNA        complement(16724..16752)
                     /note="guide RNA (gND7-I)"
     misc_RNA        complement(16767..16803)
                     /note="guide RNA (gCYb-I)"
     misc_RNA        complement(16975..17020)
                     /note="guide RNA (gRPS12-VI)"
     CDS             2332..2589
                     /note="ORF-1A"
                     /codon_start=1
                     /translation="IKLNSKESFRKEGSDQEDQRGFGNQRFDLGERSRRVKFCPRAFF
                     RAGEELDRPRKRSFSEGFEQANILTFLPTCTGGSQKSAPKV"
     CDS             2342..2641
                     /note="ORF-1B"
                     /codon_start=1
                     /translation="MVRRVFGRRDRTRRTREVSGTSDLIWENGAAEWNFAQEPFLERE
                     KSWIGRGKDRFRKGLSRPTFWLFCQLAQEGARSLHLKFSFGRFFEGRGLSRSMFD"
     CDS             2676..2969
                     /partial
                     /note="NH2 terminus uncertain"
                     /product="ORF2"
                     /codon_start=1
                     /translation="HKYLLQIGHPTKCGSLSSSLNTKIPKMVFPPQSSNVSSPPNPKS
                     VQSRSLSPLSLKTRFPKNLSRKNPALLLSKNLYLKLPSKNLPSKKPLPSPPET"
     CDS             complement(2966..3268)
                     /partial
                     /note="NH2 terminus uncertain"
                     /product="ORF10"
                     /codon_start=1
                     /translation="STYTVTNYHKKNIILFMHKILKLNTFNCTSWKIMLLLNNLYCVD
                     NYNSMYFNLNGILLWLNLLHINIIIMKYSFLMLLNNLEYLIMFFLYNLMSIKYKDV"
     CDS             5393..6481
                     /gene="CYb"
                     /note="Unedited version of CYb"
                     /codon_start=1
                     /translation="MIMKAERKEKALTSGCLLRVYGVGFSLGFFICMQIMCGVCLAWL
                     FFSCFICTNWYFVLFLWDFDLGFVMRSTHMCFTSLLFFLLYVHIFKCMVLMMLFDTHI
                     LVWAVGFMMYMFMVVMGFIGYVLPCTMMSYWGLTVFSNILATVPVIGTWLCYWIWGSE
                     YINDFTLLKLHVLHVLLPFVLMLVMFMHLFCLHYFMSSDGFCDRFAFYCERLCFCMWF
                     YLRDMFLAFLMLFYVVYFMFMNWYFVFHEESWVIVDTLKTSDKILPEWFFLFLFGFLK
                     AVPDKFTGLLLMVMLLFSLFLFMLNCMLWFVYCRSSLLWFTYSLMLFYSMFMSGFLAL
                     YVMLAYPMWMELQFWVLLLFMLVVCRLD"
     CDS             complement(7125..8456)
                     /transl_except=(pos:7125..7127,aa:other)
                     /gene="MURF1"
                     /citation=[8]
                     /note="start"
                     /codon_start=1
                     /translation="XSLFLYLIHMILFLLLYSFIILCDYTSLFYLSFDLMWLMIINMM
                     MLTILDSYMCFMFLLLFLFCFFFLFCFLNFDTRFVFMIMMMQYMMMFMFLHVMHMLFM
                     SMLFELFSLLLFLILISSRFGYKMLMLWYYYMMNLMNFMLLFVLLYYMMLNYCFYLCD
                     FCFLMFDEEWLGILCLFYMLLILFKLYIAMLILFLEQLYMRLGVFIFIYMLTFYVLFC
                     FILIMLLICFIYFYIMFIKLMIMQSITCVLIGLNSFAIVSLLFVLSVNNFSFLFLMFI
                     STKNYMFYMYLNFHLMYSLSLIMLIMLYYFFIVYNMFDMKYNENYFLINFIFFSFFNN
                     LLISIMMACMFLCIGSIPIVFGFFLKVFCLLLHLSYLGICMVFFSIIWLIIIYIFYFR
                     LMMNIFMFSYQFMGFWVVRLHILGFSNLFFMLSFSIFILFFDIINLFDLIL"
     CDS             complement(8548..9489)
                     /product="NADH dehydrogenase"
                     /codon_start=1
                     /translation="MLNIDIIIIIIIDILVVLILTGFVTLCERRILALVQMRIGPALC
                     FFGILTPITDGMKLFIKFMMFVISFDIIYLMGAMIITACCMFLGWFYFPMGFMLLLDT
                     GFTLTVMLCVHVFCSMFSTFFVGCFLFSSCFVYLSAMRTMFFSIMSESGMFLLYTTIY
                     SLDYFSFFGIKDVCVGQIYITNFYIAGVLFMSVFWVSMLLDGLKLPFDYMECESELVA
                     GLITELSGFFFVLYSVLEINHILLTTLLFASLCFGGLFICFKAMLILIFGFFYPRVIG
                     YRLKMTTAQAFILMFLFYMCVLMFIWLFTTKMMAMLF"
     CDS             complement(11181..12833)
                     /partial
                     /product="cytochrome oxidase subunit I"
                     /codon_start=1
                     /translation="YMFWLCLVCLSVSHKMIGLCYLLVAILSGFVGYVYSLFIRLELS
                     LMGCGILFGDYQFYNVLITSHGLIMVFAFMMPVMMGGLVNYFIPVMAGFPDMVFPRLN
                     NMSFWMYLAGFGCVVNGFLTEEGMGVGWTLYPTLMCMDFHSSLACDFVMFAVHLLGMS
                     SILNSMNLLGTLFCCRRKFFSFLSWSLFIWAALMTAMLLMITLPVLAGGVTLMLCDRN
                     FNTSFYDVVGGGDLMLFQHIFWFFGHPEVYIMLLPVFGLMSTIVEVIGFRCVFSTVAM
                     MYSMMLIAILGMFVWAHHMFVVGMDVDSRAYFGGVSILIGLPTCVKLFNWIYSFLYTD
                     MIITFEVYFVMMFIFMFLIGAVTGLFLSNVGMDMMLHDTYFVVGHFHYVLSLGAVVGF
                     FTGFMHFLAKWLPMELYLFWMFYFISTLFMGSNMLFFPMHSLGMYAFPRRMSDYPVSF
                     LFWSSFMLYGMLLLASLMLFLCALFCVFLFWDYCLFFVSLFVFSLYCFFYFSTWLPCV
                     MVLYLLLVDFAHMVLDYLFLILCFCFVFFMFFWQSLFLFFYM"
     CDS             12843..13085
                     /note="ORF-7"
                     /codon_start=1
                     /translation="MILTMKLMNFKMIQLISVSPFSPPFCFLPSPLFFPFLHLLPSNF
                     ESLLPLPPKSPAFPPSPFPYLPPFTIMHMCVLMYLY"
     CDS             13152..14465
                     /product="NADH dehydrogenase subunit 4"
                     /codon_start=1
                     /translation="MFKFMLVICNFMLLFLMVTLMFINYSFCLALQFNYIYMNMYLNY
                     INLWFMYFMGLIVFFLMFLLSRKLVSYSKYFYILLSYMFMFFDVTLMILIDDFMCFMM
                     LFESLFFPMCFVSLFFNFNNRFMFAMFYLVMFSSLSSMMCMIICIIIIFHFNMLNLQS
                     FMDICIFDSLYLGLYVWMLLFMMFSIKYPMWPFHVWLPELHVEVNTELSMLLASVVLK
                     MGFFGLYKFLFLSFNQLSMWFLGFMDSLVMLGLTFLAITLLFLSDYKKMIATWSVMHT
                     GMGLILLWHNDIIFLGLLMFCNLSHIMSSAFMFMMVGYMYDNYGVRMFLMLISFFGIS
                     MWSSLFLGIFLFNMDFPFMLLFYIDIFLLYGLISLSFMYMCCFYMMVLAVFLSSMYMY
                     MCLSFYSFMWLDKYLRLDLTINDMYLYFMTSMMVMLFFYLIYLLF"
     CDS             14396..14674
                     /partial
                     /note="NH2 terminus uncertain"
                     /product="ORF8"
                     /codon_start=1
                     /translation="YMFVFYNKHNSNIIFLFNLFIILVLNKRMHYFFFRPVFYKSPLP
                     PFPSPLFLTLKVKLLLRTSCNLVFCLPRLPLCPFSLLPKLMPIDLYKL"
     CDS             complement(14732..14998)
                     /partial
                     /note="NH2 terminus uncertain"
                     /product="ORF9"
                     /codon_start=1
                     /translation="YMPSYKKYTTNKAKHKKKHKKKHKHFICMYMYITMYYLNKIILM
                     ITYCLRKQFREIFLLALPPKNSFKLSSQKQTTSFLLCKFRRFRN"
     CDS             14888..16696
                     /note="presumptive protein 5"
                     /codon_start=1
                     /translation="MVYCNMYMYTNKMFMFFFMFFFMFGFICGMFFMGRHMLSFWLSV
                     VLCMFLVMSTMFSCFCLSICMYGYCFYDFCLLIMLDFCFMWLVFYCNGFYMFILYLMD
                     IVFCFMVFYAFYYMYFDMLLARFFHMFWWFVLCMNFFMLSYDFLTAYCGWELLGLFSF
                     FLMSYFWYRFYALKFGFKAFFMSKVGDVLLLLVFAITFLMNGYCVMTFYFLTFLCVDY
                     MFMVFIVFLLLTCGFTKSTQFGLHIWLPDAMEGPIPVSALIHAATLVVCGMMLISFVF
                     WCFDFWFCYFYSLIGWSSLMLVMMSLCVFYNFDVKRYVAFSTICQMSFSMFCCLSLDL
                     YVGCLFFCYHMFYKATLFMVLGVWMHFFFGLQDVRCYFFTYFCGCILARMLLMFAMLN
                     SCSLWFLCGFYCKDLLLCLLMLTSFFFMLEFLCVCLFFMFFTVMYNYFLLFFLCFVFK
                     CFCLVDTLFLLFDFECCLVYCTFCLYMCFVLLFFVLDFLYVFIFSSYCLFWSFYLYYA
                     SFFDMALFTVFMIDIIKFYMLSGVMFYFFNMDCIMFFWRVFLFITMGFLFFMFTTWYF
                     MCFYMYMCMFMWNLVMYFRYNLKYCLFFCMLFMMYM"
     CDS             14924..16696
                     /product="NADH dehydrogenase subunit 5"
                     /codon_start=1
                     /translation="MFMFFFMFFFMFGFICGMFFMGRHMLSFWLSVVLCMFLVMSTMF
                     SCFCLSICMYGYCFYDFCLLIMLDFCFMWLVFYCNGFYMFILYLMDIVFCFMVFYAFY
                     YMYFDMLLARFFHMFWWFVLCMNFFMLSYDFLTAYCGWELLGLFSFFLMSYFWYRFYA
                     LKFGFKAFFMSKVGDVLLLLVFAITFLMNGYCVMTFYFLTFLCVDYMFMVFIVFLLLT
                     CGFTKSTQFGLHIWLPDAMEGPIPVSALIHAATLVVCGMMLISFVFWCFDFWFCYFYS
                     LIGWSSLMLVMMSLCVFYNFDVKRYVAFSTICQMSFSMFCCLSLDLYVGCLFFCYHMF
                     YKATLFMVLGVWMHFFFGLQDVRCYFFTYFCGCILARMLLMFAMLNSCSLWFLCGFYC
                     KDLLLCLLMLTSFFFMLEFLCVCLFFMFFTVMYNYFLLFFLCFVFKCFCLVDTLFLLF
                     DFECCLVYCTFCLYMCFVLLFFVLDFLYVFIFSSYCLFWSFYLYYASFFDMALFTVFM
                     IDIIKFYMLSGVMFYFFNMDCIMFFWRVFLFITMGFLFFMFTTWYFMCFYMYMCMFMW
                     NLVMYFRYNLKYCLFFCMLFMMYM"
BASE COUNT     8050 a   1990 c   2437 g   8514 t
ORIGIN      1 bp upstream of EcoRI site.
        1 gaattcaaaa caaataaaac ccaaaacaaa taaaatccaa attaaattaa attaaattaa
       61 attaaattaa attaaaaaca aatttcgttg acacaacttt taacaatttg tttcatatat
      121 aatctaaatt tttgttgttg cttgttttat accgaaaagt ttgtaatatt atttttactt
      181 ttaagtaaaa gtttaattta tgttttaatt ttatttataa actacaatca ataaaatata
      241 gaaaagctta tttatgatat aatacaatac cacatgatta taaaaatatt aattataata
      301 atatttacac tatttattaa aagttagtaa aaaggaaata gaaataaata atcatactcc
      361 atatttatta attatttttc tacacttgta aaaatataga atatatttat ttttatagtg
      421 taattatagt attaaaatat taatcaaatt taattaataa gtaatattga ttttatttaa
      481 ttttaagtgt ttaattatat ttttgaatta aaattttatt atttggtatt taatatttaa
      541 aaatattata tattttagtt ttaaatttgt tgttttatat ttagtttaat atttatatat
      601 tagtaaataa tatagattta atttaaaatt tttatgaact gttatttata gtttaatatt
      661 tttagtttaa tgtttaaata tttaactaat gaaggcacag ttgttctata tgtacctata
      721 aaaaatagta aaattatttt aattaaatta ataaataatt attaacttaa cttaaaatta
      781 atattatgga aaatttaaaa attaaattat ttttctaata caattcatat aatatatata
      841 tatattaaat tgaatattaa aaatacaaat ttaatttgtt attaatacta ttcttttaaa
      901 aatgcataga gatataatat cacatataat ttattatttt aatatttaat atttgtttat
      961 acaaaagtaa ctttattgaa taaaaagaat tatttttatt aattattttt taaaaatata
     1021 aaaatattgt taataaaatt atcaagtttt aaaagcgttt attaaatgcg tcggtctaag
     1081 atttatattt aagattattc ttgtatatag atttttattt taataattct acataattaa
     1141 aaaagtacct caatatatgt ttattagtag catagtaatt tattaactaa ttattaaagt
     1201 gttccataga aaattttaaa attataacaa tctaaataaa taataaatta aaataaagat
     1261 tttaaaaaaa attaaaaaat taaaataggg caagtcctac tctcctttac aaagagaaca
     1321 ttttatatgt aattgtatgt ttgattgggg caatactata tctagttata tagaaaaaga
     1381 actataatca ttgaaataat aaaaggttcg agcaggttaa caagcattaa tactaaatgt
     1441 gtttcatcgt ctacttattg ctaataaaat tgattgttca tcaaaaatgc aattcgttag
     1501 ttgggttaaa atcgttgtaa agcagatttg tttatatatt taatttatat atatatttaa
     1561 aaattaatat tagtacgcaa ggatctatta tttgtaattg aaattaaaaa agcatgcgtt
     1621 tataataata attatacgta gtcaattgtt attattcata ttaatttttt taaaagtttt
     1681 ttaattttat attagtttat ttgtttacaa atttaaatta tatttcatta tttaggaata
     1741 gttaataata atttataatt ctgattagat ttatttgtta atgctattaa aggggtgtgg
     1801 aaaaagtgtt aaattattta tatatttaaa taataaataa aatataactt attagtcaga
     1861 aatggatgcc agccgttgcg gtaatttcta tgcttttaaa tattatacat ttattttatt
     1921 aatttgttac tatttaattt tagtcaataa aaaatattaa tatttttatt tgtttttaaa
     1981 caccgtccgg tatatgcaaa taaaaaatga cattaattat taattatatt atattatatt
     2041 tattcacata agtcaacaat atctatttac tgtttttgac aacatgataa ggattataaa
     2101 tggaattata aattttataa tcaaaactaa tttattatat taaattagca tgtttagata
     2161 aaacaataaa tttagaaggt attgttgccc accattcttt gtaataaaga caacgtgcag
     2221 taattaatat atttataaaa atatattttc tcatgttaaa cctttgttac tttttttatt
     2281 atttagaaaa gatgtaagtg ccaatataaa taaacatata taatgtatta aattaaatta
     2341 aatagtaagg agagttttcg gaaggaggga tcggaccagg aggaccagag aggtttcggg
     2401 aaccagcgat ttgatttggg agaacggagc cgccgagtga aattttgccc aagagccttt
     2461 tttagagcgg gagaagagtt ggatcggccg aggaaaagat cgttttcgga agggtttgag
     2521 caggccaaca ttttgacttt tttgccaact tgcacaggag ggagccagaa gtctgcacct
     2581 aaagtttagt tttgggagat tttttgaagg gagaggactt tcgagaagta tatttgatta
     2641 ataatttaat aaattaattt tatagacagt tctaacataa atatcttttg caaattggac
     2701 accccacaaa atgtggctcc ctatcttcct ctctaaatac caaaatcccc aaaatggtct
     2761 ttccccctca atcctcgaat gtctcttccc ccccaaatcc taaatccgtt caatctcgct
     2821 ctctctcccc cctctcctta aaaactcgct ttccaaaaaa cttatctcga aaaaaccccg
     2881 ctcttcttct ctctaaaaat ctttatctca aactcccctc aaaaaatctt ccctcaaaaa
     2941 aacctctccc ctcccccccc gaaacttaaa catctttata cttaatagat attaaattat
     3001 ataagaaaaa tataattaaa tactctaaat tatttaataa tattaaaaaa gaatatttta
     3061 taataataat attaatatgc aataagttta atcataataa aataccattt aaattaaaat
     3121 atattgaatt gtaattatca acacaataca agttatttaa taaaagtata attttccatg
     3181 aagtacaatt aaaagtattt aattttaaaa ttttatgtat aaacaaaata atattctttt
     3241 tatggtaatt agttacagtg taagtagatt agttttaata gtaatttaaa ttttatttag
     3301 ccgactacac gataaatata aaaagacact tgtatagatt tactttcgga ccccagcatc
     3361 cagcagccca tggcgtatta tgttgtttat tatatctttc tggagaattt ataacgtata
     3421 tagatgtaat tattgggtat ttacatcgag gtacagaaaa gttatgtgaa tataaaacag
     3481 tagagcagtg tttaccgatg aagactggat tatgtaagtg tagtttgtaa tgaacattta
     3541 ttatctttat gttttgaata tatgttaaga tgctgtctag caatacgttg tgcatttatg
     3601 cgtctattaa tgtgtgaatt tacgcgatgt tttaatgggc ttttatgttg ttcttgcatg
     3661 gttatggata taggttcatt atcaccaatg ttgtgatcat ttgaagagcg tgacaagtta
     3721 atgacttttt ttgatttatg ttgcggttgc agaatgcatt tagcgtttat gtgtttatta
     3781 ggtctattag atgattttgt tttcggattt atagattttt tgttaatgtt atgtatttca
     3841 tgtttatttg ttttagattt gtatgatttg ctttttgtag gtaatagact tttgtatttg
     3901 cgtttacgag gccttgcatt ttttgatata tttgacttat gctttaatag tattagtggt
     3961 tgtttatcta gatctttagg tatggtatga gatgtaagat tatatagttg ctatgaatta
     4021 tactttatgt tggtttttga ttattgtttt tgttatttag gtgatgcatt cgaccgtctt
     4081 tttttacgtt tatttgatat gcgaatgagt atccttttat gcaagcagtg tttttttgta
     4141 ggtttttttg tttttggctt tgtatgttta tttgattata tgtatgtgga tgttactata
     4201 gaaaccataa ttagtttatt ttatagttta tgatgttgta ttttaccagg ctgttctttt
     4261 gcaaatgttg aacatccaaa aggtgaatat agcctatttt tatgcttttt atatggattt
     4321 atctcacgtt tacgtattcg ttgtgcagat tttgtacata tttgtttatt agatgtaatg
     4381 atgcgtggtt tcatgttaca tgacttagta gcagttatcg gaaatgttga tgttgttttt
     4441 ggttctgttg acaggtaaat atatatatta gtattttaat ataaaaatat aaaaagtcat
     4501 tatataaaaa aggcggaggg gttttgattt ttgtttgttt tgttggtttg tcttacctgc
     4561 tatttgcata gtatatttaa ctttttgttt atgtggattg ttttgtatta tgtttggtag
     4621 ttttattttt atagattatt gttttatttg tttttttgca tgtttattat tttgtttggt
     4681 atgtttatta tgtgacttat ttgtagactc tttaagaggt ttatttgatg tatgttgctt
     4741 tatacgttgt attcagtatt gttttgtatg atttataatt agtgaattat tacttttttt
     4801 atctttattt tatgtagtat ttagtttagt tttatttgtt agtgttgaat ttgcatttgt
     4861 ttttgttata ccagtaatgt ttagttgttt aatttgtgat tttggttttg tattttattg
     4921 atattttatt gatattttta atttactaat taatacattt ttgttatttg tgagtggttt
     4981 attcgtaaat tttgttttgt ttttattttg atttcgtttt tttttatgtg ttttatttat
     5041 gttatgagta ggaatattat ttggtttttt atttttgtga aatcaagtat gagaatttgc
     5101 attattattt gtaacatgta gctgtggagt gtttgggtca atattatttt taatagattt
     5161 attacatttt agtcatgtgt ttttaggtat atttttatta tttttatgtt ttagtcgttg
     5221 ctttaatttt ttgtgtatgg atactcgttt tgttttttta tatgtagtct gtttatattg
     5281 acattttgta gattgtgttt gatttttttt attacgattt gtatattttg atgtgttaag
     5341 tgtagtatac ttatatgcat aagaaaataa ataaatttaa tttaaatttt aaataattat
     5401 aaaagcggag agaaaagaaa aggctttaac ttcaggttgt ttattacgag tatatggtgt
     5461 aggttttagt ttaggttttt ttatttgtat gcaaattata tgtggtgttt gcttagcatg
     5521 attatttttt agttgcttta tttgtacaaa ttgatatttt gtattatttt tatgagattt
     5581 tgatttagga tttgtgatac gtagtacaca tatatgtttt acatctttat tattttttct
     5641 tctttatgtg cacattttta aatgcatagt attgataata ttatttgata ctcatatttt
     5701 agtatgagct gtaggattta taatatatat atttatagta gttataggtt ttattggtta
     5761 tgtgttacca tgtacaatga tgtcgtattg agggttgact gtttttagta atattttagc
     5821 aactgtgcca gtgatcggta cctgactttg ttattgaatt tgaggaagtg aatatattaa
     5881 tgacttcact ttacttaaat tacacgtttt acacgtttta ttaccttttg tattaatatt
     5941 agtaatattt atgcatttat tttgtttaca ttactttatg agttctgatg gtttttgtga
     6001 tagatttgca ttttattgtg aacgtttatg cttttgcatg tgattttatt taagagatat
     6061 gtttttagct tttttaatat tattttatgt agtatacttt atattcataa attgatattt
     6121 tgtttttcat gaagagtcgt gagtaattgt tgacacattg aaaacatctg ataaaatttt
     6181 accagagtga ttttttctat ttttatttgg ctttttaaaa gcagtccctg ataaatttac
     6241 tggtttacta ttaatggtaa tactattatt ttcattattt ttatttatat taaattgtat
     6301 attatgattt gtttattgta gaagttcatt attgtgattt acatattcat taatattatt
     6361 ttatagtata tttatgagtg gttttttagc tttatatgta atattggcat atcccatatg
     6421 aatggagtta caattttggg tattactttt atttatgcta gttgtatgta gattagatta
     6481 attttgaatt atattatata aaaaattata tcagattaag ataaataagg gttggaagaa
     6541 attgcgaata ggaagggatc cagaaaggaa attagtaaga ccgagaagga gagaagggag
     6601 agagagattc gtgttattta atttttatgg attgattgta tattattgta ttacatattt
     6661 aaatgtttat attttatttt gtatagtttt tatgtattat atggcatttt tattttgttt
     6721 ttgcttttta ttagatttta tattatttgg tagcctttta gtcggagatg catttatgga
     6781 tgtatttttt ttacgatatt tattatgtgt tttagaatgt ttttctttat tatgtagatg
     6841 tatatctaca tttttacgaa tgttttgtaa tttattatct tcacattttt taatgttaat
     6901 gttttgtgac tttgtatatt tttttataat atttttttta ttttttataa tgtgtgattt
     6961 tatttatttt attatattta catttgcaat gttattttgt ataatttttt atttattttt
     7021 atatgcatta gatatgtttt gtgcattatt acaaatattt atattttgta atatgataat
     7081 gcagttaata atggattttt tattattatt atcttttcat taaattataa aattaagtca
     7141 aataaattaa taatgtcaaa aaatagaatg aagatgctaa aacttaatat aaaaaataga
     7201 tttgaaaatc ctaaaatatg aagacgcaca acccaaaatc ctatgaattg ataagaaaat
     7261 ataaaaatat ttattataag tctaaaatag aaaatataaa taataataag tcaaataata
     7321 gaaaaaaata ctatgcagat acctaaataa cttaaatgta gtagtaaaca aaatactttt
     7381 aaaaaaaatc caaatacaat aggaatagac ccaatgcata aaaatataca tgctatcata
     7441 atactaatta ataaattatt aaaaaagcta aaaaaaataa aattaattaa gaagtagttt
     7501 tcattatatt ttatatcaaa catgttataa acaataaaaa aataatataa tataattaat
     7561 ataataagac ttaatgaata tattaaatga aagttaagat acatataaaa tatgtagttt
     7621 tttgtagaaa taaatattaa aaataaaaaa ctaaaattgt taacactaag tacaaataaa
     7681 agactaacaa ttgcaaagct atttaatcca attaacacgc aagtaatact ttgtatgatt
     7741 ataagtttaa taaatataat ataaaagtaa ataaaacaaa taagtagtat aataagaata
     7801 aaacaaaaaa gaacataaaa agtcagcata taaataaaaa taaatacacc aagtcgtata
     7861 tataattgtt ctaaaaataa aattaatata gcaatatata atttaaataa aattaataat
     7921 atataaaata gacaaagaat accaagtcac tcttcatcaa atattagaaa gcaaaagtca
     7981 cataaataaa aacaataatt taatatcata taatataata atacgaataa tagtataaag
     8041 tttattaaat ttatcatata gtagtatcat aatattaata ttttataccc aaacctacta
     8101 gaaattaaaa tgagaaaaag aagtagacta aataattcaa aaagtattga tataaatagt
     8161 atatgtatta catgtaaaaa cataaatatt attatgtatt gcattattat aatcataaat
     8221 acaaatctgg tatcaaaatt taaaaaacaa aacagaaaaa agaaacagaa tagaaatagc
     8281 aataaaaata taaaacatat atatgagtct aaaatagtta atattattat attaataatt
     8341 attaatcata ttaaatcaaa agataaataa aatagagaag tgtaatcgca taagataata
     8401 aaactatata gcaataaaaa taaaattata tgaattaagt ataagaacaa ggatttttga
     8461 aaggagagga cagtcttaag gatttttgag gtagagattt gaggggaaag ggggggacca
     8521 gaagaacatt taaaagagtt ttcgatatta aaataacata gctattattt ttgttgtgaa
     8581 aagtcaaata aacattagta cacacatata aaataaaaat attaaaataa aagcctgtgc
     8641 agttgttatt tttaatctgt atccaataac tcttggataa aaaaatccaa aaattaaaat
     8701 aagtatagct ttaaaacaga taaataaacc accaaaacat aggcttgcaa ataaaagtgt
     8761 tgtgagtaag atgtgattga tctcaagtac agagtataaa acaaaaaaaa atcctgataa
     8821 ttcagtgatt aggcctgcta ctaattcact ttcacattcc atatagtcaa acggtaattt
     8881 taacccatct aataacatac ttactcaaaa tacacttata aataatacgc cagcaatata
     8941 aaaatttgta atataaattt gaccaacgca aacatcttta ataccaaaga agctaaagta
     9001 atctaaagaa taaatagtag tatatagtaa aaatatgccg ctttctgata taatactaaa
     9061 gaacattgtt ctcatagcag ataagtaaac aaaacaacta gagaataaaa aacagcctac
     9121 aaaaaatgtg ctgaacatac tacaaaaaac gtgaacacat aacataactg tcaatgtaaa
     9181 tccagtgtct aataataata tgaaacctat tggaaaataa aatcacccta aaaatataca
     9241 gcatgcagta ataatcatag cgcctattaa gtaaataatg tcaaagctaa tgacaaatat
     9301 tataaattta ataaataatt ttattccatc agtaataggt gtaaggatac caaaaaaaca
     9361 aagagcagga ccaattctta tttgaaccaa tgctaaaatt cttctttcac aaagcgttac
     9421 aaagccggtt aagataagta caactaaaat atcaataata ataataataa taatgtcaat
     9481 atttaacatt ttaaaatggc ttttatatta tcattttgaa tgatattttt attagattct
     9541 gttattgttt tgttatcttt tgtatgtttt gtatgtgtgt gaatatgtgc attgttgttt
     9601 tcaactgtat tattagtgtc aaaattaaat aatatatatt gtacttgaga cttcactgct
     9661 tctaagttca ttgatgttta ctgatttact attggtggta tgttttcatt aggactttta
     9721 ttacgtttat gtttactttt atattttggg catttaaatt ttgttagttt tgatttgtgt
     9781 aaagtagtgg gatttcaatg atactgagtt tattttattt ttggagaaac tacaatattt
     9841 agtaatttaa ttttagaaag tgattatatg ataggagact tacgtttatt acaatgtaat
     9901 catgtattaa ctttattaag tttagttata tataaattat gattatcagc tgtcgatgta
     9961 atacattcat ttgcaatatc aagtttagga gttaaagtag agaacctggt cgctgtaatg
    10021 aaatagttct attttcatca aataatgcca cagtatatgg acaatgtagt gaactctgtg
    10081 gtgtcttaca tggatttatg ccaatagtaa tttgttttat ataggtatat aatctatatt
    10141 ttattattat taggggaaag aaggactgag tcgtgtattt gatttattgt gtgttagaag
    10201 ttatgatttc atattatgat gatttgattt agactttatt ttatatgatt ttgtttttga
    10261 ttttgtagta tgtataactt ttatatttgt atttgtatta ggtttttttc ttagaatttt
    10321 ttttagtttt gtatttgttt tattatttat agtatttttt ggtttgtttt cattatcatt
    10381 tttatataca ggttatttta tatattatat atatatatta tataatttta tatgttattt
    10441 tttttgtttt agtattgcct atattatata gaatttttta catatttatt gtgttttata
    10501 tttatagatt ttatatcatt ttctaatcat ttaatttcat attttggtat aattaatatg
    10561 tttaatgtta tattttgttc atatttattt tgtttgtttt attttataat ttgctttata
    10621 ttttgcttta tattttttgt tatccgttgt ttatttgtga taatttatga ttttttattt
    10681 tttaattttg atatatatat atctttttta atgtgtgata taatatatat agattatata
    10741 tgctttttat taatatattt tggttttata ttttcgttta taacaggttt tttttgtttt
    10801 atatttgtat taaattatgt gtttttagtt ttattttttg tattagcatt gttttttggt
    10861 tttttatttt tatcttatgg attatttaca tttttaatat attatttttt ttgattatat
    10921 ataatatata gtcgctcatg ttttatatta ttacagtcag tagtaatatt ttttaagttt
    10981 ttatattttg atgtattttt tatttttgta tttttattaa tattatttat aatttgtttt
    11041 tttggtttct ttttaaaaga ctttttattt ttaaatattt tttttgatat gttttcagta
    11101 ttattgattt atgatgtaaa taattatagt gcattctata atagttataa ccagttttgt
    11161 gtaacacaat tattagcagt ttatatataa aaaaataaaa ataaagattg tcaaaaaaat
    11221 ataaaaaaaa caaagcagaa gcataaaatt aaaaataagt agtctaacac tatatgtgca
    11281 aagtctacaa gtaataaata taaaaccatt acacaaggta gtcaagttga aaaatagaaa
    11341 aaacagtaaa ggctaaatac aaataagcta acaaaaaata agcagtaatc tcaaaataaa
    11401 aaaacacaga aaagtgcaca taaaaataat attaagctgg caagtaataa cataccgtaa
    11461 agcataaatg aacttcagaa taaaaatgac acaggatagt cagatattct acgaggaaat
    11521 gcatacatac ctaaagaatg cattggaaaa aaaagcatat tacttcctat aaataatgtg
    11581 ctaataaagt agaacattca aaaaaggtat agttctatag gtagtcattt agcaaggaag
    11641 tgtataaaac ctgtaaaaaa tccaactacc gcacctaatg ataaaacata atgaaagtga
    11701 ccaactacga agtaagtgtc atgcagcatt atatctatgc ctacatttga taaaaaaaga
    11761 cctgtcacgg caccaattaa aaacataaaa ataaacatta taacaaagta aacttcaaat
    11821 gtaataatca tatcagtata taagaaacta taaattcagt taaatagttt aacacatgta
    11881 ggcaatccaa ttaaaattga aacaccacca aagtaagctc tagagtcaac atccatacct
    11941 actacaaaca tatgatgcgc tcagacaaac atacctaaaa tagcaattaa tatcatagag
    12001 tatatcatag caacagtact aaaaacacaa cgaaacccga taacttctac aatagtagat
    12061 attaaaccaa aaacaggaag taatataata tatacttcag gatgaccaaa aaatcaaaaa
    12121 atatgttgaa atagtattaa gtcaccacca cccacaacat cataaaatga tgtattaaaa
    12181 tttctatcac ataatattaa tgtaacacca ccagctaata caggtaaagt aattattaaa
    12241 agtatagcag ttataagagc agctcaaata aatagagatc aagataaaaa gctaaagaat
    12301 tttctacggc aacagaataa agtgccgagt aaatttatag aatttaaaat actagatata
    12361 cctagtaaat gcacagcaaa cataacaaaa tcacaggcta aacttgaatg aaaatctata
    12421 catattaaag ttggatataa tgtccatccg acacccatgc cctcttctgt taaaaaccca
    12481 ttaacgacac aaccaaatcc tgctaagtac attcaaaaac tcatattgtt aagtcgagga
    12541 aataccatat ctggaaaccc agccataaca ggaataaaat aatttacaag accacccatc
    12601 ataacaggca ttataaaagc aaaaaccata attaatccgt gagaagtaat taaaacatta
    12661 taaaactgat agtctccaaa taaaatacca catcctataa gtgaaagttc taatctaata
    12721 aataatgaat aaacatatcc aacaaatcct gaaaggattg caactaaaag ataacataaa
    12781 ccaatcattt tatgtgacac actaagacaa acgaggcaaa gtcaaaacat atattaaatt
    12841 aaataatttt gacaataaag ttaataaatt ttaaaataat ccaattaatt tccgtctcgc
    12901 ctttctcccc ccccttctgt tttctcccct cccccctctt cttccccttc ctacatcttc
    12961 tcccctctaa tttcgagtcc cttctccccc tccctccaaa atctcctgct tttcctccct
    13021 ctcctttccc ctatctcccc ccttttacaa ttatacatat atgtgtttta atgtatttgt
    13081 actaatttta ttagtctcag tcaaagtata aaaataagat taaattaaaa taattaaata
    13141 tttataaaaa aatgtttaaa tttatattag taatttgtaa ttttatatta ttatttttaa
    13201 tagttacttt aatatttatt aattatagtt tttgtttagc cttgcagttt aattatattt
    13261 atataaatat atacttaaat tatatcaatt tatgatttat atattttatg gggttaatcg
    13321 tattcttttt gatatttttg ttatctcgta aattagtgtc atatagtaaa tatttttata
    13381 ttttattatc ttatatattt atattttttg atgtaacatt aataatttta atcgatgatt
    13441 ttatgtgttt tatgatatta tttgagagtt tattttttcc aatatgtttt gtaagtttat
    13501 tttttaattt taataatcgc tttatatttg caatatttta tttagttata tttagttctt
    13561 tgagttcaat aatgtgtata attatttgta ttattattat ttttcatttt aatatattaa
    13621 atttacagag ttttatagat atttgtattt ttgatagttt atatttagga ttatatgtgt
    13681 gaatattatt atttataatg ttttctatta agtatccaat atgaccgttt catgtatgat
    13741 taccggaatt acatgtagaa gttaatacgg agttaagtat attattagcc agtgttgtgt
    13801 taaaaatagg tttttttggg ttgtataaat ttttattttt aagttttaat caattatcta
    13861 tatgattttt aggttttata gacagtcttg tgatgttagg attaacattt ttagcaatta
    13921 cactattatt tttaagtgat tataaaaaaa taattgcaac ttgatctgtt atacatacgg
    13981 gtataggttt gattttatta tggcataatg atattatttt tttaggatta cttatatttt
    14041 gtaatttatc acatattata agttcagcct ttatgtttat gatggtaggt tatatgtatg
    14101 ataattatgg agttagaata tttttaatgt taatttcatt ttttggtatt agcatatgaa
    14161 gctctttatt tttaggtatt tttttattta atatagattt cccatttatg ctattatttt
    14221 atattgacat ttttttgtta tatggattaa tttctttatc atttatatac atatgttgtt
    14281 tttatataat agtgttagct gtatttttat cttcaatata tatgtatatg tgtttaagtt
    14341 tttattcttt tatatgacta gataaatatt tacgtttaga tttaacaatt aatgatatat
    14401 atttgtattt tataacaagc ataatagtaa tattattttt ttatttaatt tatttattat
    14461 tttagtatta aataagagaa tacactattt ttttttccgt ccagtctttt acaaatctcc
    14521 tctccccccc tttccttccc ccctattttt aaccttaaag gtaaaactcc ttctgcgaac
    14581 ttcttgcaat ctcgtgttct gtctcccccg tctcccgctc tgcccttttt ccctcctccc
    14641 caaactaata cctatcgacc tatataaatt ataaatgcgt gaattttttg agtgagtttg
    14701 cttttgttat tttagggtaa aagccacgaa cctagttccg gaatcgacgg aatttgcaaa
    14761 gaagaaaaga ggttgtttgt ttttgggaac ttagcttgaa ggaatttttt ggggggagag
    14821 ccaggagaaa gatttcacgg aattgttttc gtaagcaata agtaatcatt aaaataattt
    14881 tatttagata gtatattgta atatatatat atatacaaat aaaatgttta tgtttttttt
    14941 tatgtttttt tttatgtttg gctttatttg tggtatattt tttataggaa ggcatatatt
    15001 aagtttttgg ttgtcagtgg ttttatgtat atttttagtg atgtcaacaa tatttagttg
    15061 tttttgttta agtatttgta tatatgggta ttgcttttat gatttttgtt tactcattat
    15121 gcttgatttt tgttttatat gattagtatt ttattgtaat ggtttttata tatttatttt
    15181 atatttaata gatattgtat tttgctttat agttttttat gcattttatt atatgtattt
    15241 tgatatgcta ttagccagat tttttcatat attttgatga ttcgtattat gtatgaactt
    15301 ttttatatta tcatatgatt ttttaacagc atattgtggt tgagaattat taggtttatt
    15361 ttcatttttt ttaatatcat acttttgata tagattttat gcattaaaat ttggatttaa
    15421 agcatttttt ataagtaaag taggagatgt actattactc ttagtatttg caattacatt
    15481 tttaatgaat ggttattgtg taataacatt ttatttttta acatttttgt gtgtagacta
    15541 tatatttata gtatttattg tgtttttatt attaacttgt ggttttacta agtccaccca
    15601 gtttggttta catatttgat taccagacgc tatggaagga ccaatcccag tatctgcatt
    15661 aattcatgct gctactttag tagtttgtgg tataatatta attagttttg tattttgatg
    15721 ctttgacttt tgattttgtt atttttatag tttaattggt tgatctagtt taatattggt
    15781 aatgatgagt ctttgtgtat tttataattt tgatgtaaaa aggtatgtag cttttagtac
    15841 aatttgtcaa ataagttttt caatgttttg ttgtttaagt ttagatttat atgtaggatg
    15901 tttgtttttt tgttatcata tgttttataa agccacttta ttcatagtgt taggtgtatg
    15961 aatacatttt ttttttggat tacaagatgt aagatgttat ttttttacat acttttgtgg
    16021 atgtatttta gctagaatgc tattaatatt tgcaatatta aattcatgtt ctttgtgatt
    16081 tttatgtggt ttttattgta aagatctttt attatgtctt ttaatgttaa catcattttt
    16141 ttttatatta gaatttttgt gtgtatgttt attttttata ttttttactg taatatataa
    16201 ttacttttta ttattttttt tatgttttgt atttaagtgt ttttgtctag tagatacact
    16261 ttttttactt tttgactttg agtgttgtct tgtttattgt acgttttgtt tatatatgtg
    16321 ttttgtattg ctattttttg tattagactt tttatatgta tttatttttt caagttactg
    16381 tttattttga tctttttatt tatattatgc atcatttttt gatatagcat tatttacagt
    16441 ttttataatt gatattatta agttttatat attatcgggt gttatatttt atttttttaa
    16501 tatagattgt attatgtttt tttgaagggt atttttattc attacaatgg gatttttatt
    16561 ttttatattt acaacatgat actttatatg cttttatatg tatatatgta tgtttatatg
    16621 aaatttagtt atatatttta gatataactt aaagtattgt ttattttttt gtatgttatt
    16681 tataatatac atataagaaa aaaagagtat tataatataa tatattttat atccagtcta
    16741 tcagaatatt ttataagtta ttatttaaaa tttatattat cttttaactt caagtcatat
    16801 gtgatattat tattattata ttaaaattta aataaagtaa tttacaataa tttatattaa
    16861 tttaaaatta aattaaattt aaaaacaaaa aatgatataa aaaatagaat aagtattata
    16921 aataataata ataataataa taataataat aataataata atacataaag acaaatttaa
    16981 ttttatattc aagtccacaa ttacctaatt ccataataat atataaaata tattgtatat
    17041 gtagattata aaagtatttt atgtattatt aagttaataa atattaaaaa ttaaaaatta
    17101 aaaattaaaa atttattaat aatacaaaaa atttgatatg aaatcccttg tgtgttcgtc
    17161 gaatataaaa attatgaacg aatttttgta tggtcagcaa aataatataa gttcattaaa
    17221 agtatgaacg aaattcttat gaatgtaaaa ttataaaaat cctatatcaa atataaaaat
    17281 tatgaacgaa tttctgtatg tgggcaagca aaaattatga acggatttct gtatgaacaa
    17341 caaaaattat gaaaacttta tatcaaaaat aaaaattatg aacgaatttt tgtatggcca
    17401 gtaaaaatta tgagaatcct atattaaata taaaaattat gaacgaattt ctgtattggc
    17461 agtaaagatt atgaacgggt ttctgtatga acagcaaaaa ttatgaacga atttctgtgg
    17521 tgtgtatatt aaacatgaac gattcctgta cggatagtaa aaattttagg aacgcatttc
    17581 tgtatggacc gtaaaaattc tgggctgaat ataaaactta tgaacgggtt gtctgtatga
    17641 acgaccaaaa aatgaacggg tgtctgtata cattaaataa aattaaaata aatagcatac
    17701 tgttaaaaca gatagaattg aaattaaata ttgacttatt taccttttta aaattttatt
    17761 taaaaaatta ttaatcaatt tgaatcaagt taaatttccg taaattttga cagaaagttc
    17821 cataaaatta aattaaatta aattaaatta aattaaatta tataaatcag aacaaattga
    17881 gataaaattt gaatttaaaa aatttaacac aaaatttgca aagaccctat aataatataa
    17941 taatataata atataataat ataataatat aataatataa taatataata ataatataat
    18001 ataatataat ataataatat aataatacaa ataatataat aatataatag tataataata
    18061 taataatata ataatataat aatataataa tataataata taataatata ataatataat
    18121 aatataataa tataaataat ataataatat aataatataa taatataata atataataat
    18181 ataataatat aatattatca agcttgttat aaaaaacttt gttttttata acaagcttga
    18241 tactctcggt atggttttaa aaataaatca aatttgatat ggtttcggct tggaattgac
    18301 tgctcgcctt ttaattttcc attaaaatcc caccgattta tgattagaca acttaaaaga
    18361 taattaaatt aagttatttt catattaaaa caagttattc ccatattaaa acaagttatt
    18421 cccatattaa aacaagttat tcccatatta aaacaagtta ttcccatatt aaaacaagtt
    18481 attcccatat taaaataaat ctttaaatga ccacaaattt tgacagagtt ctataaaatt
    18541 agacaaacgt acttaaaact gtcgacaaat catataatta ctatatgaaa tcgtagttaa
    18601 ttaataaagc acaaaaacaa aataaatcca aaataaaatc aaataaaatc gaaatcaaat
    18661 aaaattaaat ttaaaacaaa taaaaccgaa attaaattaa attaaattaa attaaattaa
    18721 attaaattaa attaaattaa attcaaaaca aataaaatcc aaaacaaatt aaaataattt
    18781 tacaaaattg acaaacttgt acttagaaac attgacaaat tatatagtta ctatatgaaa
    18841 tcgtagttaa ttaataaatt acaaaaacaa aataaatcca aaattaaatt aaattaaatt
    18901 aaattaaatt aaaataaatt aaattaaaat taaattcaaa acaaataaaa tccaaaacaa
    18961 ataaaataca aattaaatta aattaaatta aattaaatta aattaaattc aaaacaaata
    19021 aaattcaaaa cgaataaaag ccaaaacaaa ttaaaataaa ttttgacaga cagtttcaca
    19081 aaattgacaa acttgtactt agaaatattg acaaattata taattactat atgaaatcgc
    19141 agttaattaa taaattacaa aaacaaaata aatccaaaat taaattaaat taaattaaat
    19201 taaattaaat taaattaaat taaattaaat taaattaaat taaattaaat caaaattaaa
    19261 ttcaaaacaa ataaaatcca aaacaaatac aaattaaatt aaattaaatt aaattaaatc
    19321 aaaattaaat tcaaaacaaa taaaatccaa aacgagtaaa agccaaaaca aattaaaata
    19381 aattttgaca gacattccac aaaattgaca aacttgtact tagaaatatt gacaaatcat
    19441 ataattacta tatgaaatcg cagttaatta ataaattaca aaaacaaata aatccaaaat
    19501 aaaatcaaat aaaatcgaaa ttaaaaaaaa ttaagtttaa aacaaataaa accgaaatta
    19561 aattaaatta aattaaatta aattaaatta aattaaatta aattaaatta aattaaatta
    19621 aattaaatta aattaaatta aattaaattc aaaacaaata aaatcgaaat taaaaataaa
    19681 aaaattaaaa taaaaactaa aatcaaatca aaattaaaaa ataaattgaa ataaaatttg
    19741 aataaaaaaa ttaacaaaaa tctgcaaaga cccctataat atataatata taatatataa
    19801 tataataaat aatataataa tataataata tatataataa tataataata atataataat
    19861 ataataatat aatataatat aataatataa taatataaat aatataataa tataataata
    19921 taataatata ataatataat aatataataa tataataata taataatata ataatataat
    19981 aatataataa tataataata taataatata ataatataat aatataacaa tataataata
    20041 taataatata ataatataaa taatataata ttatcaagct tgttataaaa aactatgttt
    20101 tttataacaa gattgatact ctcggtatgg ttttaaaaat aaatcaaatt tgatatggtt
    20161 tcggcttgga attgactgct cgccttttaa ttttccatta aaatcccacc gatttatgat
    20221 tagacaactt aaaagataat taaattaagt tattttcata ttaaaacaag ttattcccat
    20281 attaaaacaa gttattccca tattaaaaca agttattccc atattaaaac aagttattcc
    20341 catattaaaa caagttattc ccatattaaa ataaatcttt aaatgaccac aaattttgac
    20401 agagttctat aaaattagac aaacgtactt aaaactgtcg acaaatcata taattactat
    20461 atgaaatcgt agttaattaa taaattacaa aaacaaaata aatccaaaat taaattaaat
    20521 taaattaaat taaattaaat tcaaaacaaa taaaatccaa aacaaataaa atacaaatta
    20581 aatttaaaac aaataaaacc gaaattaaat taaattaaat taaattaaat taaattaaat
    20641 taaattaaat taaattaaat taaattaaat taaattcaaa acaaataaaa gccaaaacaa
    20701 attaaaatca ttttccgtaa attttgacag aaattttaca aaattgacaa acttgtactt
    20761 agaaatattg acaaattata tagttactat atgaaatcgt agttaattaa taaattacaa
    20821 aaacaaaata aatccaaaat taaattaaat taaattaaat taaattaaat taaattaaat
    20881 taaattaaat taaattaaat taaattcaaa acaaataaaa tcgaaaacaa ataaaaccca
    20941 aaacaaatta aaataaattt tgacagacag tcccacaaaa ttgacaagct t
//