From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: E.coli shikimic acid kinase I (aroK) gene, complete cds.
Date: 28 Jul 92 10:07:15 GMT
Distribution: bionet
Checksum: 53425     5

LOCUS       ECOAROK      2225 bp ds-DNA             BCT       28-JUL-1992
DEFINITION  E.coli shikimic acid kinase I (aroK) gene, complete cds.
ACCESSION   M76389 M76677
KEYWORDS    aroK gene; shikimic acid kinase I.
SOURCE      Escherichia coli (strain K-12) DNA.
  ORGANISM  Escherichia coli
            Prokaryotae; Gram-negative facultatively anaerobic rods;
            Enterobacteriaceae.
REFERENCE   1  (bases 1 to 2225)
  AUTHORS   Lobner-Olesen,A. and Marinus,M.G.
  TITLE     Identification of the gene (aroK) encoding shikimic acid kinase I
            of Escherichia coli
  JOURNAL   J. Bacteriol. 174, 525-529 (1992)
  STANDARD  full staff_entry
REFERENCE   2  (bases 1 to 2225)
  AUTHORS   Lobner-Olesen,A., Boye,E. and Marinus,M.G.
  TITLE     Expression of the Escherichia coli dam gene
  JOURNAL   Mol. Microbiol. 6, 1841-1851 (1992)
  STANDARD  full staff_entry
FEATURES             Location/Qualifiers
     promoter        1523..1550
                     /gene="aroK"
     -35_signal      1523..1527
                     /gene="aroK"
     -10_signal      1545..1550
                     /gene="aroK"
     misc_feature    1558
                     /note="mRNA start site"
                     /gene="aroK"
     promoter        1735..1764
                     /gene="aroK"
     -35_signal      1735..1740
                     /gene="aroK"
     -10_signal      1758..1764
                     /gene="aroK"
     misc_feature    1771
                     /note="mRNA start site"
                     /gene="aroK"
     CDS             1791..2225
                     /product="shikimic acid kinase I"
                     /gene="aroK"
                     /codon_start=1
                     /translation="MRFQFMSCRASLSEAGLSLTNSLSSTEKMAEKRNIFLVGPMGAG
                     KSTIGRQLAQQLNMEFYDSDQEIEKRTGADVGWVFDLEGEEGFRDREEKVINELTEKQ
                     GIVLATGGGSVKSRETRNRLSARALSFILKRPSKSNLHARSG"
     misc_feature    2002..2225
                     /note="This sequence overlaps the 5' end of ecoaroB
                     (Accesion number X03867)"
                     /citation=[1]
BASE COUNT      579 a    502 c    654 g    490 t
ORIGIN
        1 agatctatgc cggattagcg aacttagcca gtggcgctat caggggatgg tagggcgagg
       61 cgagcgcatc atcggtgtaa taaaagacgg gcaaaagaaa tggcgacggg tgcagcaaaa
      121 cgatgtgctg gaaaacggct ggacaatttt acacgtgacg ccagacgtac cttaacgctg
      181 ggtaccggga caaactgcga accgccacaa tggttgtggc aacggcaagg agatacaaat
      241 gaagcaatgg atagccgcac tactgttgat gctgataccc ggcgtacagg cggcaaagcc
      301 gcaaaaagtg acgctgatgg tggatgacgt tccggtagct caggtgttgc aggcgctggc
      361 tgaacaggag aagttgaacc tggtcgtgtc gccagacgtc agcggtacgg tgtcgttaca
      421 tctaacagat gttccctgga agcaggcact acaaactgta gtgaaaagcg ccggactgat
      481 aacgcggcag gaaggcaaca ttctctcagt gcattccatt gcctggcaga ataacaatat
      541 cgccgccagg aggcggagca ggcgcgggcg caggcaaatc tgccgctgga aaatcgcagt
      601 ataaccctgc aatacgccga cgcgggagaa ctggcgaaag cgggggagaa gctactgagt
      661 gccaaaggga gtatgaccgt cgataaacgc accaatcgcc ttttgctacg agataacaaa
      721 acggcgttaa gcgcgcttga acagtgggta gcgcaaatgg atctgccggt cgggcaggtt
      781 gagctgtcgg cgcatattgt caccattaat gaaaaaagtt tgcgtgagtt aaggcgtgaa
      841 atggacgctg gccgatgcgc aacacgctgg tggcgttggg caagtcacca cgcttggtag
      901 cgacctctcc gtagcgacgg cgacaacgca tgtcggtttt aacattgggc gcatcaacgg
      961 acgcttgctg gatcttgagc tttccgcgct cgaacaaaaa cagcagctgg atattatcgc
     1021 cagtccgcgt ctgctggcct cacatcttca gcctgccagc attaaacagg ggagcgaaat
     1081 tccatatcag gtttccagcg ggaaagtggc gcacgtcggt ggaatttaaa gaggccgtcc
     1141 tgggatggag gtcacgccca cggtgttaca aaaaggtcgc atccggctta aattacacat
     1201 cagcctgaac gttccggggc aggtgctaca gcaggccgat ggcgaagtgc tggcgattga
     1261 taagcaggag atcgaaacgc aggtcgaggt caaaagcgga gaaacgttgg cgctgggcgg
     1321 catttttacc cgtaaaaata aatcgggtca ggatagcgta ccgttgcttg gcgacattcc
     1381 ctggttcggg caattatttc gtcatgacgg aaaagaagat gaacgacgcg agttagtggt
     1441 gtttatcacg ccacgactgg tttccagtga gtaaacagcc gtaaaagcgg taatgtttta
     1501 cgctgaacgt gtttcatcta tttgacgcgc gcaggtattt agcatacaag gagtaccgat
     1561 ttgagagttg gtgctcttcg ctgcctgcgt tccatgatga tgatttatca ttcaggcggc
     1621 attttgctgt cttttttacg ctaatcttac ccggtgattt atcgccagag cggtggtagc
     1681 aaggcagcgc gcttgcagcg accagatatg cagagggatg ggtgatttat tcagttgcca
     1741 aacccgctgg agtattgaga taattttcag tctgactctc gcaatatctt atgaggtttc
     1801 agttcatgtc ctgccgggcc tctctgagcg aagcgggttt atcattaacg aatagtctta
     1861 gtagtaccga aaaaatggca gagaaacgca atatctttct ggttgggcct atgggtgccg
     1921 gaaaaagcac tattgggcgc cagttagctc aacaactcaa tatggaattt tacgattccg
     1981 atcaagagat tgagaaacga accggagctg atgtgggctg ggttttcgat ttagaaggcg
     2041 aagaaggctt ccgcgatcgc gaagaaaagg tcatcaatga gttgaccgag aaacagggta
     2101 ttgtgctggc tactggcggc ggctctgtga aatcccgtga aacgcgtaac cgtctttccg
     2161 ctcgtgcgtt gtcgtttatc ttgaaacgac catcgaaaag caacttgcac gcacgcagcg
     2221 gataa
//