From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Plasmid pTAR (from Agrobacterium tumefaciens, strain 1D1422)
Date: 12 Jan 93 05:49:40 GMT
Distribution: bionet
Checksum: 07172 4

LOCUS       PT1PTAR1     1600 bp ds-DNA             BCT       11-JAN-1993
DEFINITION  Plasmid pTAR (from Agrobacterium tumefaciens, strain 1D1422)
            replicating protein (repA) gene, complete cds.
ACCESSION   M21299
KEYWORDS    oriV region; repA protein; replicating protein.
SEGMENT     1 of 2
SOURCE      A.tumefaciens (strain 1D1422) plasmid pTAR DNA, clone pUCD470.
  ORGANISM  Plasmid pTAR
            Prokaryotae.
REFERENCE   1  (bases 1 to 1600)
  AUTHORS   Gallie,D.R. and Kado,C.I.
  TITLE     Minimal region necessary for autonomous replication of pTAR
  JOURNAL   J. Bacteriol. 170, 3170-3176 (1988)
  STANDARD  full automatic
COMMENT     Draft entry and computer-readable sequence [1] kindly provided by
            C.I.Kado, 08-NOV-1988.
FEATURES             Location/Qualifiers
     repeat_region   423..430
                     /note="repeat copy A"
     repeat_region   443..450
                     /note="inverted repeat B"
     repeat_region   470..477
                     /note="inverted repeat C"
     repeat_region   524..531
                     /note="repeat D"
     repeat_region   1398..1414
                     /note="region of dyad symmetry"
     CDS             547..1299
                     /note="replicating protein repA"
                     /codon_start=1
                     /translation="MFQQIGAVQAKSGTDEPAHPCEKFPPERKCEAVFWKPLPRHEAR
                     EILLAARKYELAMKQPGKRTGPLGHVALEVLDYLTNLVDFGNGRLDPSISTIMEKIGR
                     AESCMSALYPNRQRGRRPAGAADKQRLPLEPAGARPRALLGKYVRKAAPLPDDAAQAR
                     QERHDTIKAHMDSLSPADRLRETVEDRTRAEQLAGYVERAAQNRPSGPRKAARRRQQS
                     RCSFTTPNRPRRTLPSSHPQKFGGTKGRKAFE"
BASE COUNT      391 a    449 c    470 g    290 t
ORIGIN      3 bp upstream of EcoRV site.
        1 gatatcgaag gcgtagtggc gtggcacctg ccacccgccg cagatgacat gcacccgaac
       61 tgagtgaagc agggttttgc cagccggaaa cgccatcacc gaggaaactt tgtgacgccg
      121 cttgcagaat caacgggaat cactgctaca tgtagagatg aagaatactc gtccctcatc
      181 ccgtgaaagc gttgcagcca cccgcgccag acaagcagcc agagggcttg tgaatgtcag
      241 catttgttat gccgggtgaa gtggtagcag agattgaccg tttgaaacag gaacgtgggg
      301 catcgtcccg cgccccgata ttggaagagg ccgtcaggtt ttatttagaa caaacaaggg
      361 cataaaataa aaagccccac ctttggcgag gcgggggctt tttggataag cagataaaac
      421 gatcttgaga accgtaagac agtctcaaga tagttcagaa ttaacgaact ctcaagatgt
      481 tagcgcttgc gggaacgtga ttttttgcct gccgtccatg ccctcttgag agaggacaaa
      541 ggaaccatgt tccagcagat cggcgcggtg caggcaaaat ccggcacaga cgaaccggcg
      601 cacccgtgcg agaaattccc gcctgaaagg aagtgcgagg ctgtattctg gaagccccta
      661 ccccgccacg aggcccgaga aatcctgctt gctgcacgca agtacgagct ggcgatgaaa
      721 cagccaggta agcgcaccgg ccccctcggt cacgtcgcgc tcgaagtgct ggactacctc
      781 accaatttgg ttgatttcgg gaacggccgg cttgatccgt ctatcagcac catcatggaa
      841 aaaatcggcc gcgccgagtc ttgtatgtcg gcgctatatc ccaaccggca acgagggcgc
      901 aggcccgcag gtgcagcaga caagcaacgc ctaccgcttg agcctgccgg cgcgcgccca
      961 agggcgcttc ttggcaagta tgtgaggaag gccgcccccc tgcccgacga cgcagcacag
     1021 gcccgtcagg agcgacatga taccatcaag gcgcacatgg acagcctttc gccggccgac
     1081 cgtctaaggg agaccgtaga ggacaggacg cgcgccgagc agttagcggg ctatgtggag
     1141 cgggcagcac agaaccgccc cagcgggccg aggaaggccg ccagacgccg gcagcaaagc
     1201 agatgcagtt tcactacacc caaccggcca cggcgaaccc tgccatcgag ccatccgcaa
     1261 aaatttggag gcacgaaagg ccggaaagct tttgaatgag cgtgagttca ctgaaagaac
     1321 tgaatccggt cctgattttt ataatcctgc ggattagtaa gcggacgcgc ttgttcggcc
     1381 cgctaacgcg gccctgcggc ggttccgaac cgccttaaag tgcggttcgg cggcaaccta
     1441 tacgcagttt caggaccatt cacacgtccg gataggtcgc ttggcttatg agaggtcgca
     1501 aggggaaatc ttgaagagaa tattattcca catcgccccg agaggcgtct tgcggtgcag
     1561 ctttaaggcg agcgattgtc tgtcggctga cgccgtgctt
//