From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: D.pseudoobscura Adh gene for alcohol dehydrogenase
Date: 28 Dec 92 21:27:35 GMT
Distribution: bionet
Checksum: 64670 8

LOCUS       DROADHGN56   3479 bp ds-DNA             INV       01-DEC-1992
DEFINITION  D.pseudoobscura Adh gene for alcohol dehydrogenase
ACCESSION   X62236
KEYWORDS    ADH gene; alcohol dehydrogenase.
SOURCE      Drosophila pseudoobscura DNA.
  ORGANISM  Drosophila pseudoobscura
            Eukaryota; Animalia; Metazoa; Arthropoda; Uniramia; Insecta;
            Pterygota; Neoptera; Holometabola; Diptera; Brachycera;
            Cyclorrhapha; Schizophora; Drosophiloidea; Drosophilidae.
REFERENCE   1  (bases 1 to 3479)
  AUTHORS   Schaeffer,S.W. and Miller,E.L.
  TITLE     Estimates of gene flow in Drosophila pseudoobscura determined from
            nucleotide sequence analysis of the alcohol dehydrogenase region
  JOURNAL   Genetics 132, 471-480 (1992)
  STANDARD  full automatic
COMMENT     *source: strain=SB18; *source: isolate=San Bernadino Mountains,
            California; *source: chromosome=IV section 88;
               From EMBL    entry DPADHGN56;  dated 16-OCT-1992.
FEATURES             Location/Qualifiers
     mRNA            join(40..119,915..1020,1084..1488,1549..1964)
                     /note="adult mRNA (Adh)"
     mRNA            join(882..1020,1084..1488,1549..1964)
                     /note="larval mRNA (Adh)"
     promoter        8..14
                     /note="adult promoter (Adh)"
     prim_transcript 40..1964
                     /note="adult primary transcript (Adh)"
     exon            40..119
                     /note="adult exon 1a"
     intron          120..914
                     /note="adult intron"
     promoter        846..853
                     /note="larval promoter (Adh)"
     prim_transcript 882..1964
                     /note="larval primary transcript (Adh)"
     exon            882..1020
                     /note="larval exon 1"
     exon            915..1020
                     /note="adult exon 1b"
     intron          1021..1083
                     /number=1
     exon            1084..1488
                     /number=2
     intron          1489..1548
                     /number=2
     exon            1549..1964
                     /number=3
     polyA_signal    1941..1946
     polyA_site      1964
     CDS             join(928..1020,1084..1488,1549..1815)
                     /EC_number="1.1.1.1"
                     /gene="Adh"
                     /product="alcoholdehydrogenase"
                     /codon_start=1
                     /translation="MSLTNKNVVFVAGLGGIGLDTSRELVKRNLKNLVILDRIDNPAA
                     IAELKAINPKVTITFYPYDVTVPVAETTKLLKTIFAQVKTIDVLINGAGILDDHQIER
                     TIAVNYTGLVNTTTAILDFWDKRKGGPGGIICNIGSVTGFNAIYQVPVYSGSKAAVVN
                     FTSSLAKLAPITGVTAYTVNPGITKTTLVHKFNSWLDVEPRVAEKLLEHPTQTSQQCA
                     ENFVKAIELNKNGAIWKLDLGTLEPITWTQHWDSGI"
BASE COUNT      968 a    795 c    800 g    916 t
ORIGIN      
        1 cgcagtgtat ttaaggcact gcacatgtcg aggaccacga ttattgtcta cagtgcagtc
       61 agtagttgtc aggtgcagca ggcggaccag catttcttta aatctactca atcgatcaag
      121 taagtaactg taatccaagt acagtcggcc catcccactc ggttctgccg tagctcaaga
      181 aatttcttga tagcgcagtt ccagttgtaa aacgtcgttt aaaaaaacat ctcgttggag
      241 cttgggttgg aaaacgttcg agttgggcgt agaaaatttc agatataaaa tatcaaataa
      301 gagaaaatat tctcccaaag cagctgtcca ttttttgtta aagtgataga tcaaaaattc
      361 gtatgacgta tttgccaagt gctctttatg caaataattt cctcaattct aattctacaa
      421 gaagcacttt tggctcttgt ccagaaaaaa caatttgttt acactttgcg gttgataaca
      481 gaagcggacc gacggctaag taacataaca gtcgcctgtt aagtaatgcc ctcactcgct
      541 gttagatggc cgcgcgtgtc gactccgata gaccagcaga cacgcagtag ttttcctcaa
      601 gaacctgaac tctaaacata gacataattt acgatacctt gagactcaaa taaaaataca
      661 tgtatgagtg gtattctatt cctgattaga ctcgcacaca cacacgtacg agtattaaca
      721 taaatttgcc ggcaaataac caaaacaaaa cgaacgcaaa cgatccgaac acgtaatgcg
      781 agagataaga aacagaaaag ctcgacgtga aagaagcttc tggcggaagc tcttgccgaa
      841 ctccctataa atacgagaca gaaaaccaga ataaatataa taacaaactg ttgaattgtc
      901 ccatcgattc ccagatcaaa agacagaatg tctctcacca acaagaacgt cgttttcgtg
      961 gccggcctgg gcggcattgg cctggacacc agccgggagt tggtcaagcg taatctgaag
     1021 gtaagagaga acgaattcca tggaatctat ggaatcctaa atttaaaaat tcatttattt
     1081 tagaacctgg tcatcctgga tcgcattgac aatccggctg ccattgccga actgaaggca
     1141 atcaatccca aggtgaccat caccttctat ccctacgatg tgactgtgcc cgtcgctgag
     1201 accaccaagc tcctgaagac catctttgcc caggtgaaga caatcgatgt cctgatcaac
     1261 ggtgctggca tcctggacga tcatcagatc gagcgcacca ttgccgttaa ctacacgggc
     1321 ctggtcaaca ccaccacagc cattctggac ttctgggaca agcgcaaggg cggcccaggc
     1381 ggcatcattt gcaacattgg ctccgtcacc ggtttcaatg ccatctacca ggtgcccgtt
     1441 tactccggct ccaaggcggc ggtggttaac tttacctcct ccctggcggt aagtaatcca
     1501 acgtttttcc tactccccaa aactaaatat ttctttgaaa tcccatagaa actggctccc
     1561 attactggtg tcactgcata cactgtcaat cctggcatca ctaagaccac tctggtccac
     1621 aaattcaact cgtggctgga tgtggagccc cgtgtggcgg agaagctgct cgagcatccc
     1681 acccagacct ctcagcagtg cgccgagaac tttgtgaagg ccattgagct gaacaagaac
     1741 ggtgccatct ggaagttgga tttgggcacc ttggagccca tcacatggac ccagcactgg
     1801 gactcgggca tctaaaccat cccagagact ctatgggaca tggcgtttta gcttttagtt
     1861 tctttttttc cactcaattg ttacgtatat acatacacat atggcaataa ggctgatttg
     1921 aaccccgttt tgaatatgat aatacaaaat tatatttgag aaatttcaac aaaatcgata
     1981 agagctatag ctctctttta attaaaaaaa actagataaa aagagcaatg gtcgtcagtg
     2041 gtggtcagtg gtcgttgtgg cctctatcga tttccacaca aaaactttac tgtttagtaa
     2101 tagaaaagaa cttgagaaag gcagccaaaa gaatgtacga tctgacgggt aagcatgtct
     2161 gctatgtggc tgactgcggt ggcattgcac tggagaccag caaggttctc atgaccaaga
     2221 atatagcggt gagtggtaga gtggtgtaga gagtggagag tgttccgaag tggttctggt
     2281 tcttaaacaa gtctctagtc tctagtctct tggctgctgc tggtagctgt cgaaactaat
     2341 taagttaatg actttttcat gttttgtttt tgtccgctga cttttgctgt tcaaagctac
     2401 aattaagttt atgttttgag tttgatctac ttttgcttaa gggaagtact ttcgattacg
     2461 gattgctggc atataaaaat agataccaag gaatgtgcat catatcatat tgattctatc
     2521 ttgattcttt gcccagaaac tggccatcct tcagagcgtg gaaaaccccc cggccatcgc
     2581 tcagttacaa tcgattaagc acagcacaca gatcttcttc tggaccttcg atgtgaccat
     2641 ggcccgcgag gagatgaaga agtacttcga tgaggtcatg gtccagatgg actacatcga
     2701 tgtgctgatc aatggggcca ccctgtgcga tgagcggaac attgatgcca ccatcaatac
     2761 gaatctgacc ggcatgatga acaccgtggc cactgtgctg ccctatatgg accgtaagat
     2821 gggcggttcg ggtggactga ttgtgaatgt cacctccgtc ataggactgg atccatcgcc
     2881 agtcttttgt gcgtacagtg cctcaaagtt tggtgtgatt gggttcaccc gaagtctagc
     2941 ggtgagtggg cgagatctta acttgaaaat cctctctaat gtcttccatt ctttcgcagg
     3001 atcccctgta ttacacccaa aatggtgtgg ctgtcatggc cgtctgctgt ggtcccacca
     3061 aagtgtttgt cgatcgggaa ctgactgcct tcctggaata cggccagtcc tttgccgatc
     3121 gcctgcgtcg tgccccctgc caatcgacgg ccgtctgcgg ccagaatata gtaaatgcca
     3181 tcgagagatc ggagaacgga cagatctgga ttgccgacaa gggcggcctg gagtcggtgg
     3241 ccctccactg gtattggcat atggccgatc agtttgtaaa ctatatgcag agcactgacg
     3301 atgaggatca ggagtttttt ttgggccagc gatgaggcat gccttgatcc acggaataaa
     3361 acatcaaatg tgtcacaaaa agtaggcaac ttgtttgtgg gtctggaaat agatttgaaa
     3421 tattttgagg tcatatgaat aatgacataa atgagggtgc ctgaaagtat gccaagttt
//