From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: D.pseudoobscura Adh gene for alcohol dehydrogenase
Date: 28 Dec 92 21:26:54 GMT
Distribution: bionet
Checksum: 15440 8

LOCUS       DROADHGN28   3468 bp ds-DNA             INV       01-DEC-1992
DEFINITION  D.pseudoobscura Adh gene for alcohol dehydrogenase
ACCESSION   X62208
KEYWORDS    ADH gene; alcohol dehydrogenase.
SOURCE      Drosophila pseudoobscura DNA.
  ORGANISM  Drosophila pseudoobscura
            Eukaryota; Animalia; Metazoa; Arthropoda; Uniramia; Insecta;
            Pterygota; Neoptera; Holometabola; Diptera; Brachycera;
            Cyclorrhapha; Schizophora; Drosophiloidea; Drosophilidae.
REFERENCE   1  (bases 1 to 3468)
  AUTHORS   Schaeffer,S.W. and Miller,E.L.
  TITLE     Estimates of gene flow in Drosophila pseudoobscura determined from
            nucleotide sequence analysis of the alcohol dehydrogenase region
  JOURNAL   Genetics 132, 471-480 (1992)
  STANDARD  full automatic
COMMENT     *source: strain=GB59; *source: isolate=Gundlach Bundschu Winery,
            Sonoma Valley, *source: California; *source: chromosome=IV section
            88;
               From EMBL    entry DPADHGN28;  dated 16-OCT-1992.
FEATURES             Location/Qualifiers
     mRNA            join(40..119,918..1023,1098..1502,1565..1982)
                     /note="adult mRNA (Adh)"
     mRNA            join(885..1023,1098..1502,1565..1982)
                     /note="larval mRNA (Adh)"
     promoter        8..14
                     /note="adult promoter (Adh)"
     prim_transcript 40..1982
                     /note="adult primary transcript (Adh)"
     exon            40..119
                     /note="adult exon 1a"
     intron          120..917
                     /note="adult intron"
     promoter        849..856
                     /note="larval promoter (Adh)"
     prim_transcript 885..1982
                     /note="larval primary transcript (Adh)"
     exon            885..1023
                     /note="larval exon 1"
     exon            918..1023
                     /note="adult exon 1b"
     intron          1024..1097
                     /number=1
     exon            1098..1502
                     /number=2
     intron          1503..1564
                     /number=2
     exon            1565..1982
                     /number=3
     polyA_signal    1959..1964
     polyA_site      1982
     CDS             join(931..1023,1098..1502,1565..1831)
                     /EC_number="1.1.1.1"
                     /gene="Adh"
                     /product="alcoholdehydrogenase"
                     /codon_start=1
                     /translation="MSLTNKNVVFVAGLGGIGLDTSRELVKRNLKNLVILDRIDNPAA
                     IAELKAINPKVTITFYPYDVTVPVAETTKLLKTIFAQVKTIDVLINGAGILDDHQIER
                     TIAVNYTGLVNTTTAILDFWDKRKGGPGGIICNIGSVTGFNAIYQVPVYSGSKAAVVN
                     FTSSLAKLAPITGVTAYTVNPGITKTTLVHKFNSWLDVEPRVAEKLLEHPTQTSQQCA
                     ENFVKAIELNKNGAIWKLDLGTLEPITWTQHWDSGI"
BASE COUNT      974 a    792 c    792 g    910 t
ORIGIN      
        1 cgcagtgtat ttaaggcact gcacatgtcg aggacgacga ttattgtcta cagtgcagtc
       61 agtagttgtc aggtgcagca ggcggaccag catttcttta aatctactca atcgatcaag
      121 taagtaactg taatccatgt cgactgtact gcccatccca ctcggttctg cagtaactca
      181 agaaatttct tgatagcgca gttccagttg taaaacgtcg cttaaaaaaa catctcgttg
      241 gagcttgggt tgaaaaacgt tcgagttggg cgcagaaaat ttcaaatata aaatatcaaa
      301 aaagagaaaa tatcttccca aagcagctgt ccattttttg ttaaagtgat agatcaaaaa
      361 ttcgtatgac gtatttccca agtgctcttt atgcaaataa tttcctcaat tctaattcta
      421 caagaagcac ttttggctct tgtccagaaa aaacaatttg tttacacttc tcggttgata
      481 acagaggcgg accgacggct aagtaacata acagtcgcct gttaagtaat gccctcactc
      541 tctgttagat ggccgcgcgt gtcgactccg atagaccagc agacacgcag tagttttcct
      601 caagaacctg aactctaaac atagacataa tttacgatac cttgagactc aaataaaaat
      661 acatgtatga gtggtattct attcctgatt agactcgcac acacacacgt acgagtatta
      721 acataaattt gccggcaaat aaccaaaaca aaacgaacgc aaacgatccg aacacgtaat
      781 gcgagagata agaaacagaa aagctcgacg tgaaagaagc ttctggcgga agctcttgcc
      841 gaactcccta taaatacgag acagaaaacc agaataaata taataacaaa ctgttgaatt
      901 gtcccatcga ttcccagatc aaaagacaga atgtctctca ccaacaagaa cgtcgttttc
      961 gtggccggtc tgggcggcat tggcctggac accagccggg agttggtcaa gcgtaatctg
     1021 aaggtaagag agagagagag caatcccttt tctatggaat ctatggaatc ctaaatttaa
     1081 aaatttattt tttttagaac ctggtcatcc tggatcgcat tgacaatccg gctgccattg
     1141 ccgagctgaa ggcaatcaat cccaaggtga ccatcacctt ctatccctac gatgtgactg
     1201 tgcccgtcgc tgagaccacc aagctcctga agaccatctt tgcccaggtg aagacaatcg
     1261 atgtcctgat caacggtgct ggcatcctgg acgatcatca gatcgagcgc accattgccg
     1321 ttaactacac gggcctggtc aataccacca cagccattct ggacttctgg gacaagcgca
     1381 agggcggccc aggcggcatc atttgcaaca ttggctccgt caccggtttc aatgccatct
     1441 accaggtgcc cgtttactcc ggctccaagg cggcggtggt taactttacc tcctccctgg
     1501 cggtaagaaa tcctacaact atcctaacta tcccaaaact aaatatttct ttaaaatccc
     1561 atagaaactg gctcccatta ctggtgtcac tgcttacact gtcaatcctg gcatcactaa
     1621 gaccactctg gtccacaaat tcaactcgtg gctggatgtg gagccccgtg tggcggagaa
     1681 gctgctcgag catcccaccc agacctctca gcagtgcgcc gagaactttg tgaaggccat
     1741 tgagctgaac aagaacggtg ccatctggaa gttggatctg ggcaccttgg agcccatcac
     1801 atggacccag cactgggact cgggcatcta aagatcatcc cagagactct atgggacatg
     1861 gcgttttagc ttttagtttc tttttttcca ctcaattgtt acgtatatac atacacatat
     1921 ggcaataagg ctgatttgaa ccccgttttg aatatgataa tacaaaatta tatttgagaa
     1981 atttcaacaa aatcgataag agctatagct ctcttttaat taaaaaaaac tagataaaaa
     2041 gagcaatggt cagtggtggt cagtggtcgt tgtggcctct atcgatttcc acacaaaaac
     2101 tttactgttt agtaatagaa aagaacttga gaaaggcagc caaaagaatg tacgatctga
     2161 cgggtaagca tgtctgctat gtggctgact gcggtggcat tgcactggag accagcaagg
     2221 ttctcatgac caagaatata gcggtgagtg gagagtggag agtgttccga agtggttctg
     2281 gttcttaaac aagtctctag tctctagtct cttggctgct gctggtagct gtcgaaacta
     2341 attaagttaa tgactttttc atgttttgtt tttgtccgtt caaagctaca attaagttta
     2401 tgttttgagt ttgatctact tttgcttaag ggaagtactt tcgattacgg attgctggca
     2461 tataaaaata gataccaagg aatgtgtatc atatcatatt gattccatct tgattatttg
     2521 cccagaaact ggccatcctt cagagcgtgg aaaacccccc ggccatcgct cagttacaat
     2581 cgattaagca cagcacacag atcttcttct ggaccttcga tgtgaccatg gcccgcgagg
     2641 agatgaagaa gtacttcgat gaggtcatgg tccagatgga ctacatcgat gtgctgatca
     2701 atggggccac cctgtgcgat gagcggaaca ttgatgccac catcaatacg aatctgaccg
     2761 gcatgatgaa caccgtggcc actgtgctgc cctatatgga ccgaaagatg ggcggttcgg
     2821 ggggactgat cgtgaatgtc acctccgtca taggactgga tccatcgcca gtcttttgtg
     2881 cgtacagtgc ctcaaagttt ggtgtgattg ggttcacccg aagtctagcg gtgagtgggc
     2941 gagatcttaa cttgaaaatg ctctctaata tcttccattc ttccgcagga tcccctgtat
     3001 tacacccaaa atggtgtggc tgtcatggcc gtctgctgtg gtcccaccaa agtgtttgtc
     3061 gatcgggaac tgactgcctt cctggcatac ggccagtcct ttgccgatcg cctgcgtcgt
     3121 gccccctgcc aatcgacggc cgtctgcggc aagaatatag taaatgccat cgagagatcg
     3181 gagaacggac agatctggat tgccgacaag ggcggcctgg agtcggtggc cctccactgg
     3241 tattggcata tggccgatca gtttgtgaac tacatgcaga gcactgacga tgaggatcag
     3301 gagttttttt tggggcagcg atgaggcatg ccttgatcca cggaataaaa catcaaatgt
     3361 gtcacaaaaa gtaggcaact tgtttgtggg tttggaaata gatttgaatt attttgaggt
     3421 catatgaata atgacataaa tgagggtgcc tgaaagtatg ccaagttt
//