From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: P.aeruginosa genes aprD, aprE, aprF, aprA, aprI for alkaline
Date: 3 Jan 93 22:08:19 GMT
Distribution: bionet
Checksum: 34867 15

LOCUS       PSEAPRAPG    7413 bp ds-DNA             BCT       21-DEC-1992
DEFINITION  P.aeruginosa genes aprD, aprE, aprF, aprA, aprI for alkaline
            protease secretion apparatus, alkaline protease, inhibitor.
ACCESSION   X64558
KEYWORDS    alkaline protease; aprA gene; aprD gene; aprE gene; aprF gene;
            aprI gene; protease inhibitor; secretion apparatus.
SOURCE      Pseudomonas aeruginosa DNA.
  ORGANISM  Pseudomonas aeruginosa
            Prokaryotae; Gracilicutes; Scotobacteria; Pseudomonadaceae.
REFERENCE   1  (bases 1 to 7413)
  AUTHORS   Duong,F., Lazdunski,a., Murgier,M. and Cami,B.
  TITLE     Nucleotide sequence of a cluster of genes involved in synthesis and
            secretion of alkaline protease in Pseudomonas aeruginosa
  JOURNAL   Gene 121, 47-54 (1992)
  STANDARD  full automatic
COMMENT     *source: strain=PAO1; *source: clone=E.coli 1046 [pJA67]; *source:
            map_position=63min;
               From EMBL    entry PAAPRAPG;  dated 03-DEC-1992.
FEATURES             Location/Qualifiers
     CDS             142..1923
                     /function="component of alkalineprotease secretion
                     apparatus"
                     /gene="aprD"
                     /codon_start=1
                     /translation="MARLGSSVTNEIKQALAASRGALRSVAAFSGVINLLMLVPSLYM
                     LQVYDRVLSSANEVTLLMLTLMALGVFVFMGALEALRSFVLVRVSERFDGQLHGRIYA
                     AAFERNLRAGGQEASQALHDLTTLRQFITGQALFAFFDAPWFPVYLLVIFLFDPWLGL
                     LSLVGALALMALAWFNERATRAPLAKAGELSIKSGQLASNNLRNAEVIEAMGMLGSMR
                     GRWERLHQAFLDQQSLASERAARINALSKYLRIALQSLVLGLGAWLAVEGRITPGMMI
                     AGSILMGRALGPIDQLIGVWKQWGAARDAYRRLSGLLDEFPARERRMELPEPRGHLLL
                     ESLDAAPPGSEARTLRGLTLAIPAGSVVGVIGPSGSGKSSLARVVLGIWPTLHGSVRL
                     DGAEIRQYERETLGPRIGYLPQDIELFAGTVAENIARFGEVQADKVVEAARLAGVHEL
                     VLRLPQGYDTVLGVGGAGLSGGQRQRIALARALYGAPTLVVLDEPNSNLDDSGEQALL
                     AAIQALKARGCTVLLITHRAGVLGCADRLLALNAGQLHLYGERDQVLAALNNQRAASA
                     SQQRADYRVAGYGAPQVVAAPRQGGVE"
     CDS             1920..3218
                     /function="component of alkalineprotease secretion
                     apparatus"
                     /gene="aprE"
                     /codon_start=1
                     /translation="MTRTVKRDENAYARLGWLLVLFGFGGALLWAAFAPLDQGVAVPA
                     TVIISGQRKSVQHPLGGVVKHILVRDGQHVEAGEPLIRMEPTQARANVDSLLNRYANA
                     RLNQARLQAEYDGRRTLEMPAGLAEQAPLPTLGERLELQRQLLHSRQTALANELSALR
                     ANIEGLRAQLEGLRQTEGNQRLQQRLLNSQLSGARDLAEEGYMPRNQLLEQERQLAEV
                     NARLSESSGRFGQIRQSIAEAQMRIAQREEEYRKEVNGQLAETQVNARTLWEELSSAR
                     YELRHAEIRAPVSGYVAGLKVFTDGGVIGPGELLMYIVPNSDSLEVEGQLAVNLVDRI
                     HSGLPVEMLFTAFNQSKTPRVTGEVTMVSADRLLDEQNKQPYYRVRAQVDAAAMGKLK
                     GLQIRPGMAVQVFVRTGERSLLNYLFKPLFDRAHVALAEN"
     CDS             3222..4667
                     /function="component of alkalineprotease secretion
                     apparatus"
                     /gene="aprF"
                     /codon_start=1
                     /translation="MTMRRLMTWLFGAFLLLLREDAFALGLLDGYHLALENDPQFQAA
                     IQEHEAGRQYRALGRAALLPRLVYSYNRGRSWSDVTQTTTRGDFKEDRDYDSYVSTLS
                     LQQPLFDYEAFSRYRKGVAQALLSDERFRSQSQELLVRVLEAYTGALLAQDQIELARA
                     QKRSYREQFQLNQRQFERGNGTRTDTLETQARFNLAQAQEIEARDSQDAALRELERLV
                     GAPLEIADLAPLGERFQVRPLSPASYTAWRDLALAENPELASLRHAVDVARYEVEQNR
                     ADFLPRLGLYASTGKSKSGSENTYNQRYETDSVGIQLSVPLFSGGETLAATRQATHRM
                     EKSHYDLDDKVRETLNQVRKMYNQSSSSAAKIRAYEMTVDSARTLVMATRKSIAAGVR
                     VNLDLLNAEQALYSAMNELSKAKYDYLTAWARLRFYAGVLDEADLELVAANFVSGETP
                     ARRRDCATTDCPAPLHTLSKTDTEENRSALN"
     CDS             5026..6465
                     /gene="aprA"
                     /product="alkaline protease"
                     /codon_start=1
                     /translation="MSSNSLALKGRSDAYTQVDNFLHAYARGGDELVNGHPSYTVDQA
                     AEQILREQASWQKAPGDSVLTLSYSFLTKPNDFFNTPWKYVSDIYSLGKFSAFSAQQQ
                     AQAKLSLQSWSDVTNIHFVDAGQGDQGDLTFGNFSSSVGGAAFAFLPDVPDALKGQSW
                     YLINSSYSANVNPANGNYGRQTLTHEIGHTLGLSHPGDYNAGEGDPTYADATYAEDTR
                     AYSVMSYWEEQNTGQDFKGAYSSAPLLDDIAAIQKLYGANLTTRTGDTVYGFNSNTER
                     DFYSATSSSSKLVFSVWDAGGNDTLDFSGFSQNQKINLNEKALSDVGGLKGNVSIAAG
                     VTVENAIGGSGSDLLIGNDVANVLKGGAGNDILYGGLGADQLWGGAGADTFVYGDIAE
                     SSAAAPDTLRDFVSGQDKIDLSGLDAFVNGGLVLQYVDAFAGKAGQAILSYDAASKAG
                     SLAIDFSGDAHADFAINLIGQATQADIVV"
     CDS             6714..7109
                     /gene="aprI"
                     /product="Inhibitor"
                     /codon_start=1
                     /translation="MSASAKLSRMVCLLCGFFSTGISMASSLILLSASDLAGQWTLQQ
                     DEAPAICHLELRDSEVAEASGYDLGGDTACLTRWLPSEPRAWRPTPAGIALLERGGLT
                     LMLLGRQGEGDYRVQKGDGGQLVLRRATP"
BASE COUNT     1258 a   2475 c   2490 g   1190 t
ORIGIN      
        1 aatccagctg atcggcgccg acccggcgcc ggagcatcag gccctggccg cctgacggcg
       61 agggaacgcc cggcacaggg aggtgccatc cgggctcatt cgggattcca gcggcagtcg
      121 tggcagaagt gagcgcgctc gatggcaagg ctcggttcca gcgttaccaa cgaaatcaaa
      181 caggccctgg cggccagccg cggcgcattg cgcagcgtgg cggcgttctc cggggtgatc
      241 aacctgctga tgctggtgcc ctcgctgtac atgctgcagg tgtacgaccg cgtgctgtcc
      301 tcggccaacg aagtcaccct gttgatgctg accctgatgg cgcttggcgt gttcgtcttc
      361 atgggcgcgc tggaggcgct gcgcagcttc gtcctggtgc gggtcagcga acgcttcgac
      421 ggccaattgc acgggcggat ctacgccgcc gccttcgaac gcaacctgcg cgccggcggc
      481 caggaggcca gccaggcgct gcacgacctg accaccctgc gccagttcat caccggccag
      541 gcgctgttcg ccttcttcga cgcaccctgg ttcccggtct acctgctggt gatcttcctc
      601 ttcgacccct ggctcggcct gctctcgctg gtcggcgcgc tggccctgat ggccctcgcc
      661 tggttcaacg aacgcgccac ccgtgcgccg ctggccaagg ccggcgagtt gtcgatcaag
      721 tccggccaac tggccagcaa caacctgcgc aacgccgagg tgatcgaggc gatgggcatg
      781 ctcggcagca tgcgcgggcg ctgggaacgg ctgcaccagg cgttcctcga ccagcagagc
      841 ctggccagcg agcgcgcggc gcggatcaac gcgctgagca aatacctgcg catcgccctg
      901 caatccctgg tgctcggcct cggcgcctgg ctggcggtgg agggacgcat caccccgggg
      961 atgatgatcg ccggctcgat cctgatggga cgtgcgctgg ggcctatcga ccaactgatc
     1021 ggcgtgtgga agcagtgggg cgcggcgcgc gatgcctatc gccggctgag cggcctgctc
     1081 gacgaattcc ccgcccgcga gcggcgcatg gaattgcccg agccgcgtgg ccacctgctg
     1141 ctggaatcgc tggacgcggc gccgcccggc agcgaggcgc gcaccctgcg tggcctgacc
     1201 ctggcgatcc cggccggctc ggtggtcggc gtgatcggtc cgtccggctc cggcaagtcg
     1261 agcctggcgc gggtagtgct gggcatctgg ccgaccctgc acggcagcgt gcgcctggac
     1321 ggcgccgaga tccgccagta cgagcgcgag accctcggcc cgcgcatcgg ctacctgccg
     1381 caggacatcg aactgttcgc cggcacggtg gcggaaaaca tcgcccgctt cggcgaggtg
     1441 caggccgaca aggtggtgga ggcggcgcgc ctggccgggg tccatgaact ggtcctgcgc
     1501 ctgccgcagg gctacgacac cgtgctcggc gtcggcggcg ccgggctttc cggcggccag
     1561 cgccagcgca tcgccctggc ccgggcgctg tacggcgcgc cgaccctggt ggtgctcgac
     1621 gaacccaact cgaacctcga cgacagcggc gagcaggcgc tgctggcggc gatccaggcg
     1681 ctcaaggcgc gtggctgcac ggtgctgctg atcacccacc gggccggcgt gctgggttgc
     1741 gccgaccgcc tgctggcgct gaacgccggc cagttgcacc tgtacggcga gcgcgaccag
     1801 gtgctcgccg ccttgaacaa ccagcgcgcc gcgtcggcgt cgcagcagcg cgcggactac
     1861 cgggtggccg gctacggcgc gccgcaggtg gtggccgcgc cgcgccaggg aggtgtggaa
     1921 tgacccgcac ggtgaaacgc gacgagaacg cctatgcgcg actgggctgg ctgctggtcc
     1981 tgttcggctt cggcggcgcg ctgctctggg ccgccttcgc gccgctcgac cagggcgtgg
     2041 cggtgccggc cacggtgatc atctccggcc agcgcaagtc ggtgcagcat ccgctgggcg
     2101 gggtggtcaa gcacatccta gtgcgcgacg gccagcatgt ggaggcgggc gagccgctga
     2161 tccgcatgga accgacccag gcccgggcca acgtcgattc gctgctcaac cggtacgcca
     2221 acgcgcggct caaccaggcg cgcctgcagg ccgaatacga cggccggcgg accctggaga
     2281 tgcccgcggg gctggccgag caggccccgc tgccgaccct cggcgagcgc ctggagttgc
     2341 agcggcagtt gctgcacagc cgccagaccg cgctggccaa cgaactctcc gcattgcggg
     2401 cgaacatcga ggggctgcgc gcccagctcg aagggttgcg ccagaccgag ggcaaccagc
     2461 gcctgcaaca acgcctgttg aacagccagt tgagcggtgc gcgcgacctc gccgaggaag
     2521 gctacatgcc gcgcaaccag ttgctcgaac aggagcgcca actggccgag gtgaacgccc
     2581 ggctatcgga gagcagcggt cgcttcgggc agatccgcca gagcatcgcc gaggcgcaga
     2641 tgcgcatcgc ccaacgcgag gaggagtacc gcaaggaagt caacgggcaa ctggcggaaa
     2701 cccaggtcaa tgcgcggacg ctctgggagg agctgtcctc ggcgcgctac gaactgcgcc
     2761 acgccgagat ccgcgcgccg gtcagcggct acgtggccgg cttgaaggtg ttcaccgatg
     2821 gcggggtgat cggccccggc gaattgctga tgtacatcgt gcccaacagc gacagcctgg
     2881 aagtggaggg ccagctcgcg gtgaacctgg tcgaccggat ccacagcggg ctgccggtgg
     2941 agatgctgtt caccgccttc aaccagagca agaccccgcg ggtcaccggc gaggtgacca
     3001 tggtcagcgc cgaccgcctg ctcgacgagc agaacaagca gccctactac cgcgtgcgcg
     3061 cccaggtcga cgcggccgcg atgggtaagc tgaagggcct gcagatccgc ccggggatgg
     3121 cggtgcaggt gttcgtccgc accggcgagc gctcgctgct caattacctg ttcaagcccc
     3181 tgttcgaccg cgcccacgtg gcgctggcgg agaactgacc gatgaccatg cgccgcctga
     3241 tgacctggct gttcggcgcc ttcctgttgc tgctgcgcga ggacgccttc gccctcggcc
     3301 tgctggacgg ctatcacctg gccctggaga acgacccgca gttccaggcc gcgatccagg
     3361 agcacgaggc cggcaggcaa taccgtgcgc tcggccgcgc ggcgctgctg ccgcgcctgg
     3421 tgtacagcta caaccgtggg cgcagctggt ccgatgtgac ccagaccacc acccgcggcg
     3481 atttcaagga agaccgcgac tacgacagct acgtctccac cctcagcctg caacagccgt
     3541 tgttcgacta cgaggccttc agccgctacc gcaagggcgt ggcccaggcg ctgctgtccg
     3601 acgagcgctt ccgcagccag agccaggaac tgctggtccg ggtcctggaa gcctataccg
     3661 gcgcgctgct ggcccaggac cagatcgaac tggcgcgggc gcagaaacgc tcctaccgcg
     3721 agcagttcca gctcaaccag cgccagttcg agcgcggcaa cggcacccgc accgacaccc
     3781 tggagaccca ggcccgcttc aacctggccc aggcccagga aatcgaggcg cgggacagcc
     3841 aggatgccgc cctgcgcgaa ctggagcggc tggtcggggc gccgctggag atcgccgacc
     3901 tggcgccgct cggcgaacgc ttccaggtgc gcccgctgag ccccgccagc tacaccgcct
     3961 ggcgcgacct ggcgctggcc gagaatcccg agctggcctc gctgcgccac gcggtggacg
     4021 tggcccgcta cgaggtcgag cagaaccgcg ccgacttcct gccgcgcctg ggcctgtacg
     4081 ccagcaccgg caagtccaag tccggttcgg agaacaccta caaccagcgc tacgagaccg
     4141 acagcgtcgg catccagttg agcgttccgc tgttctccgg cggcgagacc ctggcggcga
     4201 cgcgccaggc cacgcatcgc atggagaaga gccattacga cctggacgac aaggtccgcg
     4261 agaccctcaa ccaggtgcgc aagatgtaca accagagcag cagcagcgcg gcgaagatcc
     4321 gcgcctacga gatgaccgtg gattcggcgc ggacgctggt gatggccacg cgcaagagca
     4381 tcgccgccgg ggtgcgggtc aacctcgacc tgctgaacgc cgagcaggcg ctgtacagcg
     4441 cgatgaatga gctgtccaag gccaagtacg actacctcac ggcctgggca cgcctgcgtt
     4501 tctacgccgg ggtgctggac gaagccgacc tggaactggt ggcggcgaac ttcgtttccg
     4561 gcgagacgcc ggcgcgccgc cgcgactgcg cgacgacgga ttgcccggcg ccgctgcaca
     4621 ccctgtcgaa gaccgatacg gaggaaaacc ggagcgcgct caattgagac cgcgaaggac
     4681 gtgcgggcaa ttgttcgcct ggcgtgaagt cgatgtcccg ggcgggtcga cagaggtcga
     4741 acaatacgca tggaattgtt ttttcaataa ggcgctattt atatgaaacg gtttttataa
     4801 tattccagtg tcttcggtag tttgtctttc ctgcatgatt tcatatatgc ggaattcgtt
     4861 cgtgcatatg ctttaagtgc agcttaattt ttttcatgaa acctgtggac attggaaaat
     4921 gagctgtgtc agtttggact tcggcctgat ctggccgata actgcaatta tccatccgat
     4981 aattgtatcc gagttatttc ctttacaact taaagggata tgacaatgtc cagcaattct
     5041 cttgcattga aaggtcgtag cgatgcgtat acccaggtag acaacttcct gcatgcctat
     5101 gcgcggggcg gggacgaatt ggtcaatggc catccgtcct ataccgtcga ccaggcggcg
     5161 gagcagatcc tccgcgaaca ggcgtcttgg cagaaagcgc cgggcgactc ggtgctgacc
     5221 ctgtcctatt cgttcctgac caaaccgaac gacttcttca atacgccgtg gaagtatgtc
     5281 agcgatatct actcgctggg caagttcagc gccttttccg cgcagcagca ggcccaggcc
     5341 aagttgtcgc tgcaatcctg gtcggacgtc accaatatcc acttcgtcga cgccggccag
     5401 ggcgatcagg gcgacctgac cttcggcaac ttcagcagta gtgtcggcgg tgcggcgttc
     5461 gccttcctgc cggatgtacc ggatgcgctc aaggggcaat cctggtacct gatcaacagc
     5521 agctacagcg ccaacgtcaa tccggccaac ggcaactacg gacgccagac cctgacccac
     5581 gagatcggcc ataccctggg cctgagccac cccggcgact acaacgccgg cgagggcgat
     5641 cccacctacg ccgacgctac ctacgccgag gacacccgcg cctattcggt gatgagctac
     5701 tgggaagagc agaacaccgg ccaggacttc aagggcgcct attcctcggc accgctgctg
     5761 gacgacatcg cggcgatcca gaagctctac ggggccaacc tgaccactcg caccggcgac
     5821 acggtgtacg gcttcaactc caacaccgag cgcgacttct atagcgccac ctcgtccagt
     5881 tccaagctgg tgttctcggt gtgggacgcc ggcggcaacg acaccctgga cttctccggc
     5941 ttcagccaga accagaagat caacctcaac gagaaggcgc tgtccgatgt cggcgggttg
     6001 aagggcaatg tgtcgatcgc tgccggggtc accgtggaaa acgccatcgg cggctcgggt
     6061 agcgacctgt tgatcggcaa cgacgtggcc aacgtgctca agggcggcgc cggcaacgac
     6121 atcctctacg gcggcctcgg cgcggaccag ctgtggggtg gcgcgggagc cgacaccttc
     6181 gtctacggcg atatcgccga gtcctccgcg gcggcgccgg ataccctgcg cgacttcgtc
     6241 agcggccagg acaagatcga cctgtccggg ctggacgcct tcgtcaacgg cgggctggtg
     6301 ctgcaatacg tcgacgcctt cgccggcaag gccggccagg cgatcctgtc ctacgacgcg
     6361 gcgagcaagg ccggcagcct ggcgatcgac ttcagcgggg acgcccatgc cgatttcgcg
     6421 atcaatctga tcggccaggc gacccaggcc gacatcgtcg tctgacgcgc tgacacgggc
     6481 gcggttcggt gcggcggtac gaaccgcggc ccttcggccg gccgggcact gttcgaattg
     6541 ataggtgtct tccacaggag cgaagttgtg aactgtggcg cgcccgtggg cctgcgaccc
     6601 ggcggacgaa cggaggtccg cggcgccctg gccggcgcct ggcgttcgaa gcgagggtgg
     6661 cgggcggcgt tcgccgtgtt atccgtagtt ccaggcttga aggagatctc tcgatgtcgg
     6721 caagcgcgaa gctctccagg atggtttgct tgctctgcgg ttttttcagc acaggtatca
     6781 gtatggccag cagtctgatt cttctcagcg cttccgatct cgccgggcaa tggaccctgc
     6841 agcaggacga ggcgcccgcg atctgccacc tggagctgcg cgacagcgaa gtggcggaag
     6901 ccagtggcta cgacctgggc ggcgataccg cctgcctcac gcgctggctg cccagcgagc
     6961 cgcgcgcctg gaggcctacc ccggccggga tcgcgctgct cgaacgcggc ggcctgaccc
     7021 tgatgctcct cggtcgccag ggcgagggcg actaccgggt gcagaagggc gacggcgggc
     7081 agttggtgct gcgccgcgcg acgccctagc tcgcttgcgg gtcagaccac gaagcgaccg
     7141 atcatgccct tgaggtcgtt ggccaggcgc gacagctcgt gctggcgccg cgggtctgct
     7201 cggcgccctg ggtggtctgc acggcaacgt cgcggatgtt caccaggttg tgatcgacct
     7261 cgcgggcgac gtgggcctgc tcctcggcag cgctggcgat caccagggtg cgctcgttga
     7321 tccgcgtggc gtgctcggcg atctcgccca gggcgtggtc ggcggcctcg gcctcgtcga
     7381 gcatctgccg ggcgcgctgg tcgttctcgc gca
//