From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Oryzias latipes hatching enzyme constituent protease (lce) mRNA,
Date: 3 Jan 93 22:18:19 GMT
Distribution: bionet
Checksum: 12886 4

LOCUS       ORZLCE        936 bp ss-mRNA            VRT       23-DEC-1992
DEFINITION  Oryzias latipes hatching enzyme constituent protease (lce) mRNA,
            complete cds.
ACCESSION   M96169
KEYWORDS    hatching enzyme; protease; zinc metalloprotease.
SOURCE      Oryzias latipes pre-hatching embryo exocrine gland cDNA to mRNA.
  ORGANISM  Oryzias latipes
            Eukaryota; Animalia; Chorqata; Vertebrata; Osteichthyes;
            Actinopterygii; Atheriniformes; Cyprinodontoidei; Oryziatidae.
REFERENCE   1  (bases 1 to 936)
  AUTHORS   Yasumasu,S., Yamada,K., Akasaka,K., Mitsunaga,K., Iuchi,I.,
            Shimada,H. and Yamagami,K.
  TITLE     Isolation of cDNAs for LCE and HCE, two constituent proteases of
            the hatching enzyme of Oryzias Latipes, and concurrent expression
            of their mRNAs during development
  JOURNAL   Dev. Biol. 153, 250-258 (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     sig_peptide     34..93
                     /gene="lce"
                     /codon_start=1
     misc_feature    94..246
                     /gene="lce"
                     /note="propeptide"
     misc_feature    121..123
                     /note="glycosylation site"
     misc_feature    193..195
                     /note="glycosylation site"
     mat_peptide     247..846
                     /gene="lce"
                     /codon_start=1
     misc_binding    547..549
                     /bound_moiety="zinc"
     misc_feature    550..552
                     /note="active site"
     misc_binding    559..561
                     /bound_moiety="zinc"
     misc_feature    664..666
                     /note="glycosylation site"
     CDS             34..849
                     /gene="lce"
                     /product="protease"
                     /codon_start=1
                     /translation="MDLLAKASVLLLLLLSLSNAQTDNMEEAENGSSKEEIDESELED
                     VSSIIFRMNNNSMEELLEGDLVLPKTRNAMKCFGAPDSCRWPKSSNGIVKVPYVVSDN
                     YESDEKETIRNAMKEFAEKTCIHFVPRNNERAYLSLEPRFGCKSMMGYVGDKQVVVLQ
                     RFGCIKHAVIQHELLHALGFYHEHTRSDRDQHVKINWENIIKDFTHNFDKNDTDNLGT
                     PYDYGSIMHYGRTAFGKDRKETITPIPNPKAAIGQTERMSDIDILRVNKLYKC"
BASE COUNT      288 a    210 c    225 g    213 t
ORIGIN      
        1 gtcagaagca tcagcattta aaaaaaaaaa aaaatggacc tgctggccaa agcatctgtg
       61 ctgctgttgc tgctcctgag cctcagcaac gctcaaactg acaatatgga ggaagcagaa
      121 aacggttcat caaaggagga aatagacgag tctgaactgg aggacgtgtc ctccatcatc
      181 ttcagaatga acaacaactc tatggaggaa ctgttggaag gagatcttgt tcttcccaaa
      241 accaggaatg ccatgaagtg ctttggcgct ccagatagct gccgctggcc aaagtcttcc
      301 aatggcatcg tgaaggttcc ttatgtggtt agcgacaact atgaaagtga cgagaaggaa
      361 accattcgga acgccatgaa ggagtttgca gaaaaaacct gcattcactt tgttcctcgc
      421 aacaatgaga gggcctacct gagccttgaa cccagatttg gctgcaagtc tatgatgggc
      481 tatgttggtg acaaacaagt ggtggtgctt cagcggtttg gctgcataaa gcacgccgtc
      541 atccagcatg agctcctgca tgctctgggt ttctaccacg agcacactcg gagcgaccgc
      601 gaccagcatg taaaaatcaa ctgggaaaac atcattaaag atttcacaca caactttgat
      661 aagaatgata ccgacaatct gggcaccccg tatgactatg gctccatcat gcactatgga
      721 agaactgcct ttggaaaaga cagaaaggag accataaccc ccatccctaa ccccaaagct
      781 gccattggcc aaacagagag gatgtcggac atcgatatac ttcgagtcaa taagctttac
      841 aaatgttgag atgacatgag ctgaaaatga tatttgatgc ttcatgtggg tgtacgacat
      901 taaagtgaat cggtaaaggg gagcgtttat ccttgt
//