From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Mycobacterium intracellulare (MI85) catalase-peroxidase gene,
Date: 27 Jan 93 05:50:39 GMT
Distribution: bionet
Checksum: 18305 6

LOCUS       MSGCATPXDS   2452 bp ds-DNA             BCT       26-JAN-1993
DEFINITION  Mycobacterium intracellulare (MI85) catalase-peroxidase gene,
            complete cds.
ACCESSION   M86741
KEYWORDS    broad-spectrum peroxidase; catalase; peroxidase.
SOURCE      Mycobacterium intracellulare (serovar 14) (library: lambda gt11)
            DNA.
  ORGANISM  Mycobacterium intracellulare
            Prokaryotae; Firmicutes; Mycobacteria; Mycobacteriaceae.
REFERENCE   1  (bases 1 to 2452)
  AUTHORS   Morris,S.L., Nair,J. and Rouse,D.A.
  TITLE     The catalase-peroxidase of Mycobacterium intracellulare: nucleotide
            sequence analysis and expression in Escherichia coli
  JOURNAL   J. Gen. Microbiol. 138, 2363-2370 (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     -35_signal      57..61
                     /note="Shine-Dalgarno"
     repeat_region   2323..2357
                     /rpt_type=inverted
     CDS             67..2307
                     /function="binds hydrogen peroxide"
                     /product="catalase-peroxidase"
                     /codon_start=1
                     /translation="MSSDTSSSRPPQPDSGTASKSESENPAIPSPKPKAHAPLTNRDW
                     WPDQVDVSSLHPHSPLSNPLGDDFDYAAEFAKLDVEALKADMISLMTTSQDWWPADYG
                     HYGGLFIRMSWHAAGTYRIHDGRGGAGQGMQRFAPLNSWPDNASLDKARRLLWPIKKK
                     YGNKISWADLITYAGNVALESMGFKTFGFGFGREDVWEPEEILWGEEEEWLGTDKRYS
                     GERELAQPYGATTMGLIYVNPEGPEGKPDPIAAAIDIRETFGRMAMNDEETAALIVGG
                     HSFGKTHGAGDADLVGPEPEAAPIEQQGLGWKSSYGTGSGKDAITSGLEVVWTPTPTK
                     WDNSFLETLYGYEWELTKSPAGAWQFTAKDGAGAGTIPDPFGGAGRAPTMLVTDISLR
                     ESPIYADITRRWLDHPEELADAFAKAWYKLLHRDMGPISRYLGPWVAEPQLWQDPVPA
                     VDHELVDDNDVAALKKKVLDSGLSIPQLVKTAWSAAASYRNTDKRGGANGGRLRLQPQ
                     RSWEVNEPSELDKVLPVLEKIQQDFNASASGGKKISLADLIVLAGSAAVEKAAKDAGY
                     EISVHFAPGRTDASQESTDVESFAVLEPRADGFRNYIRPGEKAPLEQLLIERAYLLGV
                     TGPEMTVLVGGLRALGANHGSSKHGVFTDRPGALTNDFFVNLLDMGTEWKASETAENV
                     YEGRDRASGALKWTATANDLVFGSNSVLRGLVEVYAQDDAHGKFVEDFVAAWVKVMNS
                     DRFDLK"
BASE COUNT      450 a    822 c    842 g    338 t
ORIGIN      
        1 gactgcaggc tcctagatca cagcccgtga tcgcagcccg attcacccac cctcgaaagg
       61 aatgctgtgt catctgatac atccagtagc cgcccacccc aaccagacag cgggacggct
      121 agcaagagcg aaagcgaaaa cccagcaatc ccttcaccca agccgaaggc acatgccccg
      181 ctgaccaacc gggactggtg gcccgaccag gtcgacgtct cgtcgctgca cccgcactcg
      241 ccgctgtcca acccgctcgg cgacgacttc gattacgccg cggagttcgc caagctcgac
      301 gtcgaggcgc tcaaggccga catgatctcg ctgatgacca cgtcgcagga ctggtggccg
      361 gccgactacg gccactacgg cggcctgttc atccggatga gctggcacgc cgcgggcacc
      421 taccgcatcc acgacggccg cggcggcgcc ggccagggca tgcagcgctt cgccccgctg
      481 aacagctggc cggacaacgc gagcctggac aaggcccggc ggctgctgtg gccgatcaag
      541 aagaagtacg gcaacaagat ctcctgggcc gacctgatca cctacgcggg caacgtggcc
      601 ctggagtcga tgggcttcaa gacctttggc ttcggcttcg gccgcgagga cgtctgggag
      661 cccgaagaga tcctctgggg agaagaggag gaatggctgg gcaccgacaa gcgctactcg
      721 ggtgagcgcg agctcgcgca gccctacggc gcgacgacca tgggcctgat ctacgtcaac
      781 cccgaaggcc cggagggcaa gcccgacccg atcgccgcgg cgatcgacat ccgcgagacg
      841 ttcggccgga tggcgatgaa cgacgaggag accgccgcgc tgatcgtcgg cgggcacagc
      901 ttcggcaaga cccacggcgc cggtgacgcc gacctggtgg gccccgagcc ggaggccgcc
      961 ccgatcgaac agcaggggct gggctggaag agctcgtacg gcaccggttc gggcaaggat
     1021 gcgatcacca gcggcctcga ggtggtctgg acgcccaccc cgacgaagtg ggacaacagc
     1081 ttcctggaga cgctgtacgg ctacgaatgg gagctgacca agagccccgc cggggcgtgg
     1141 cagttcaccg ccaaggacgg cgcgggtgcg ggcacgatcc ccgacccgtt cggcggtgcc
     1201 gggcgggccc cgacgatgct ggtgaccgac atctcgttgc gggagtcccc gatctacgcc
     1261 gacatcacgc ggcgctggtt ggaccacccc gaggagctgg ccgacgcgtt cgccaaggcg
     1321 tggtacaagc tgttgcaccg cgacatgggc ccgatcagcc gctacctggg cccgtgggtc
     1381 gccgagccgc agctgtggca ggaccccgtc cccgccgtcg accacgagct ggtcgacgac
     1441 aacgacgtcg cggcgctgaa gaagaaggtg ctcgactccg ggttgtccat cccgcagttg
     1501 gtcaagacgg cctggtcggc ggcggcgagc tatcgcaaca ccgacaagcg cggcggagcg
     1561 aacggtggcc ggctgcgcct gcagccgcag cgcagctggg aggtcaacga gccctccgag
     1621 ctcgacaagg tgctgccggt gctggaaaag atccagcagg acttcaacgc ctcggcatcc
     1681 ggtggcaaga agatctcgct ggccgacctg atcgtgctgg cgggctcggc ggccgtcgag
     1741 aaggcggcca aggacgccgg ctacgagatc tcggtgcact tcgcgccggg ccgcaccgac
     1801 gcctcgcagg agagcaccga cgtggagtcg ttcgcggtgc tcgaaccgcg ggccgacgga
     1861 ttccgcaact acatccggcc gggtgagaag gcgccgctcg agcagctact gatcgagcgt
     1921 gcgtacctgc tcggcgtgac cggtccggag atgacggtgc tcgtcggcgg tctgcgtgcc
     1981 ctcggtgcca accacggcag cagcaagcac ggggtcttca ccgacaggcc gggcgcgttg
     2041 accaacgact tcttcgtcaa cctgctcgac atgggcaccg agtggaaggc gtcggagacc
     2101 gcggagaacg tctacgaggg acgcgaccgg gcttccggcg cgctcaagtg gaccgcgacc
     2161 gcgaacgacc tcgtcttcgg ctccaactcg gtgctgcgtg ggctggtcga ggtctacgcc
     2221 caggatgacg cccacgggaa gttcgtcgag gacttcgtcg cggcgtgggt gaaggtcatg
     2281 aacagcgacc ggttcgacct caaataacag tcggatagcg agcgacaccc cgcgggccac
     2341 cggttcgcgg ggtgtcgtgc gttcggatca gcgcgccaga tcccattgtc cgtagtcggg
     2401 cgcgaggacg tcgtcgacgt ccacgttgat cccgccgagc agcgggcccg gg
//