From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: S.cerevisiae MET, LEU4, and POL1 genes encoding MET4 protein,
Date: 24 Jul 92 10:10:56 GMT
Distribution: bionet
Checksum: 08558    15

LOCUS       YSCMET4      3479 bp ds-DNA             PLN       24-JUL-1992
DEFINITION  S.cerevisiae MET, LEU4, and POL1 genes encoding MET4 protein,
            alpha-isoproplymalate (alpha-IPM) synthetase (partial), and DNA
            polymerase alpha (partial)
ACCESSION   Z12126
KEYWORDS    DNA polymerase; DNA polymerase alpha; LEU4 gene; MET gene;
            MET4 protein; alpha-isoproplymalate synthetase; pol1 gene.
SOURCE      Saccharomyces cerevisiae DNA.
  ORGANISM  Saccharomyces cerevisiae
            Eukaryota; Fungi; Eumycota; Hemiascomycetes; Endomycetales;
            Saccharomycetaceae.
REFERENCE   1  (sites)
  AUTHORS   Pizzagalli,A., Valsasnini,P., Plevani,P. and Lucchini,G.
  TITLE     DNA polymerase I gene of Saccharomyces cerevisiae: Nucleotide
            sequence, mapping of a temperature-sensitive mutation, and protein
            homology with other DNA polymerases
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 85, 3772-3776 (1988)
  STANDARD  full staff_review
REFERENCE   2  (bases 660 to 3302)
  AUTHORS   Mountain,H.A., Bystrom,A.S. and Korch,C.
  TITLE     The methionine pathway specific transcriptional activator MET4 of
            Saccharomyces cerevisiae is regulated by the general amino acid
            control.
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
REFERENCE   3  (bases 1 to 3479)
  AUTHORS   Korch,C.T.
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
REFERENCE   4  (sites)
  AUTHORS   Thomas,D., Jacquemin,I. and Surdin-Kerjan,Y.
  TITLE     MET4, a leucine zipper protein, and centromere-binding factor 1 are
            both required for transcriptional activation of sulfur metabolism
            in Saccharomyces cerevisiae
  JOURNAL   Mol. Cell. Biol. 12, 1719-1727 (1992)
  STANDARD  full staff_review
COMMENT     *source: strain=S288C; *source: clone_library=YCp50 (Rose et al.,
            GENE 60, 237-243, 1987); *source: clone=pHAM65, pHAM93, and pHAM94;
            *source: chromosome=XIV, Left arm, between LEU4 and POL1; *source:
            is_macronuclear=N; *source: is_proviral=N;
            From EMBL    entry SCMET4;  dated 22-MAY-1992.
FEATURES             Location/Qualifiers
     CDS             complement(<1..69)
                     /function="Synthesis of alpha-isopropylmalate"
                     /EC_number="4.1.3.12"
                     /product="Alpha-isoproplymalate (alpha-IPM) synthetase"
                     /gene="LEU4"
                     /label=SCLEU4
                     /codon_start=1
                     /translation="MVKESIIALAEHAASRASRVIPP"
     protein_bind    164..170
                     /function="DNAbinding sequence of general amino acid
                     activatorGCN4"
                     /bound_moiety="General amino acid geneactivator/regulator
                     protein GCN4"
                     /standard_name="GCN4 DNA binding sequence"
                     /label=GCN4-1
     protein_bind    212..219
                     /function="DNA binding site of HAP2 and HAP3 proteins"
                     /bound_moiety="HAP2 and HAP3 proteins"
                     /standard_name="HAP2-HAP3 DNA binding site"
                     /label=HAP2-HAP3
     protein_bind    385..389
                     /function="DNAbinding sequence of general amino acid
                     activatorGCN4"
                     /bound_moiety="General amino acid geneactivator/regulator
                     protein GCN4"
                     /standard_name="GCN4 DNA binding sequence"
                     /label=GCN4-2
     protein_bind    484..491
                     /function="DNAbinding sequence of general amino acid
                     regulatorGCN4"
                     /bound_moiety="General amino acid geneactivator/regulator
                     protein GCN4"
                     /standard_name="GCN4 DNA binding sequence"
                     /label=GCN4-3
     misc_signal     719..726
                     /function="Upstream activating sequence for Metgenes and
                     CPF1 binding"
                     /phenotype="Activator ofMET genes, maintain chromosome
                     stability"
                     /standard_name="UAS(Met)"
                     /label=UAS-Met-1
                     /citation=[4]
                     /citation=[2]
                     /citation=[3]
                     /citation=[1]
     misc_signal     762..769
                     /function="Upstream activating sequence for Metgenes and
                     CPF1 binding"
                     /phenotype="Activator ofMET genes, maintain chromosome
                     stability"
                     /standard_name="UAS (Met)"
                     /label=UAS-MEt-2
                     /citation=[4]
                     /citation=[2]
                     /citation=[3]
                     /citation=[1]
     protein_bind    803..818
                     /function="Activation of genes uponheat shock"
                     /bound_moiety="Yeast heat shock element bindingprotein
                     HSF1"
                     /standard_name="HSE"
                     /label=HSE
                     /citation=[4]
                     /citation=[2]
                     /citation=[3]
                     /citation=[1]
     protein_bind    858..889
                     /function="Recognition site of MAT-alpha 2 protein for
                     generegulation"
                     /bound_moiety="MAT-alpha 2 protein"
                     /standard_name="MAT-alpha 2"
                     /label=MAT-alpha_2a-2b
                     /citation=[4]
                     /citation=[2]
                     /citation=[3]
                     /citation=[1]
     TATA_signal     871..876
                     /citation=[4]
                     /citation=[2]
                     /citation=[3]
                     /citation=[1]
     TATA_signal     934..940
                     /citation=[4]
                     /citation=[2]
                     /citation=[3]
                     /citation=[1]
     CAAT_signal     996..999
     protein_bind    1075..1083
                     /function="Transcription activation upon amino
                     acidstarvation"
                     /bound_moiety="General amino acidactivator/regulator
                     protein GCN4"
                     /standard_name="GCN4"
                     /note="TATA elements (871, 934) are too distantfrom the 5'
                     end of the mRNA, but GCN4 elements canfunction as TATA
                     elements (Chen & Struhl, EMBO J8, 261-268, 1989; Mosch et
                     al. (EMBO J 9,2951-2957, 1990)."
                     /label=GCN4-4
                     /citation=[4]
                     /citation=[2]
                     /citation=[3]
                     /citation=[1]
     mRNA            1100..>3068
                     /function="5' end of least expressedtranscript of MET4
                     gene"
                     /gene="MET4"
                     /evidence=EXPERIMENTAL
                     /label=transcript-3
                     /citation=[4]
                     /citation=[2]
                     /citation=[3]
                     /citation=[1]
     prim_transcript 1135..>3068
                     /function="5' end of primary transcriptof the MET4 gene"
                     /gene="MET4"
                     /evidence=EXPERIMENTAL
                     /label=transcript-1
                     /citation=[4]
                     /citation=[2]
                     /citation=[3]
                     /citation=[1]
     mRNA            1136..>3068
                     /function="5' end of a minor transcriptof MET4 gene"
                     /gene="MET4"
                     /evidence=EXPERIMENTAL
                     /label=transcript-2
                     /citation=[4]
                     /citation=[2]
                     /citation=[3]
                     /citation=[1]
     CDS             1164..3068
                     /function="Transcriptional activator ofmethionine genes"
                     /note="The ATG (met) codon at1050-1052 is in-frame but
                     cannot be used since itis upstream of the transcriptional
                     start points."
                     /product="MET4"
                     /gene="MET"
                     /label=MET4
                     /codon_start=1
                     /translation="MGSTNSLDQFVATASSSSSLVTSSENRRPLIGDVTNRGNTNLYD
                     HAVTPEILLEQLAYVDNFIPSLDNEFSNVDWNVNTTHNNANNNGADTFSSINANPFDL
                     DEQLAIELSAFADDSFIFPDEDKPSNNNNNSNNGNDDHSNHDVLHEDPSTNNRQRNPH
                     FLTQRRNTFLTSQYDQSKSRFSSKNKRNGNNGETNNFGDNMQNNHPFEPNFMGSPSQF
                     PADATNMTSIDHGGFTNVDITSTENNTTGDNGVDALSNLLHRTTHTPNRSSPLSNVTS
                     AQNSSSQQRKHSESKVDSNSDNNSSNKAPNITVPDYSIIPTSVLVTLLPRVNVPNGAY
                     NSLISAGFDNDQIDAIAAIMAYHHQKKIRENNSNNNKNINTNDSQEAPILKNINELLS
                     VLIPPSPAETRGPTTLSTSPSFNEHGVVAEASFLSSILELGIKHPKSNNIHNQRQPSR
                     NDHKISRESDGNNGNDNVHHNNAVIKSSTTRGDEIAKIRSEPTLNASSSDHKENSLKR
                     SHSGDLKNKKVPVDRKYSDNEDDEYDDADLHGFEKKQLIKKELGDDDEDLLIQSKKSH
                     QKKKLKEKELESSIHELTEIAASLQKRIHTLETENKLLKNLVLSSGETEGIKKAESLK
                     KQIFEKVQKE"
                     /citation=[4]
                     /citation=[2]
                     /citation=[3]
                     /citation=[1]
     conflict        2763^2764
                     /note="replace(2764^2763,'tatg')"
                     /citation=[4]
                     /citation=[1]
                     /partial
     conflict        3024
                     /note="replace(3024,'gg')"
                     /citation=[4]
                     /partial
     CDS             3402..>3479
                     /function="DNA Replication"
                     /note="Our base 2724 corresponds to base 1 of thesequence
                     SCPOL1M (EMBL databank) reported byA.Pizzagalli et al.
                     Proc. Natl.Acad. Sci. USA85:3772-3776 (1988)."
                     /product="DNAPolymerase alpha"
                     /gene="POL1"
                     /label=SCPOL1
                     /codon_start=1
                     /translation="MSSKSEKLEKLRKLQAARNGTSIDDY"
BASE COUNT     1173 a    770 c    635 g    901 t
ORIGIN
        1 tggaggaata actcttgagg ctctggaggc cgcatgctca gcaagagcaa taatactctc
       61 tttaaccatg attgttcctt gtctttttgt aaggaaaaag tctacagtaa acttctagtg
      121 tgagaatcct tttttttctt caaaaaatac cctttgaaag aactgagtca cttacacgta
      181 agtttctttt tactggtcaa gttgaaaaat aaccaataaa tgattatgga aagaacgttg
      241 aaagaggaga gaggctgacc caagaggaga aaacatcgaa cctacgcgac tccatagcgc
      301 acatctccca tgcgcccgtg cttatatata tataaatata catacacaca tacatgcacg
      361 cacatacatg cacgcgcatg acagtgactg gccgttactg tacaattttt tcagccaagt
      421 atgacacaca ttcaactcag cttttctgag gccttctttc ttttcctgcg cgtcggtaga
      481 gcgatgacta acctactact gtctcagagc cggtcccgct ccggtagcaa tcctggggct
      541 ggtcataaca gccgagtgga agtgtcaaag cggagaacag aagcataagc tcaatcgctg
      601 gacatacgga tgcttatata cgtcttattg tcgttgaaaa atatcgaatt ttttacttca
      661 tttatcgagg cttcttcgag cacttttccg ctatggcttt ttccccgttt ccttttaatc
      721 acgtgcgcgg gtagcacccg gcacacagct ggtgtctcgt cgcacatgct attgtgtgtc
      781 atcgggccac acaagcatat tgcttgaatt ttctttcatc gttcaactta aatccaccca
      841 atctagatgt agccgtagca tgtaataacg tatatccttg tttacatgca tctgtgccag
      901 gtgaaacggt ctgtttgaac gcacatcatt tcatatatta gtcaactcct gaaggtcttc
      961 ttgcctgtcc gtcaactgtt tagacagact ctcgtcaata aagcgcactt ctgataagca
     1021 cttttattcc tttttttcca ctgtgaacga tgaagcagga gcagtcccac gaaggcgact
     1081 catacagcac ggaattcata aatctctttg gcaaagatac cgcaacacac cctagcagca
     1141 acaacggtgc taataataat ggcatgggga gcacgaactc gttggaccag tttgtggcaa
     1201 cagcctcatc gtcatcttct ctggtgacca gcagcgagaa taggcgcccc ctaataggtg
     1261 acgttaccaa taggggcaac actaacctat atgaccacgc tgtcacgcca gaaatactct
     1321 tagaacagtt ggcctacgtg gataacttca taccatctct ggataacgag ttctctaatg
     1381 tggattggaa tgtgaatacc acccataata atgcaaacaa taatggcgcg gacactttca
     1441 gcagcataaa tgcaaatcct tttgacttgg atgaacaact agccattgag ttgagtgcgt
     1501 ttgccgacga ttctttcatc ttcccagacg aggataagcc gagcaataac aataacaaca
     1561 gcaataatgg taatgacgac catagcaacc acgacgtatt gcatgaggac ccttctacca
     1621 ataatagaca aagaaatcct cacttcttga ctcaaagaag gaatactttc ctaacttccc
     1681 aatacgacca atcaaagtct cgattttcgt ccaaaaacaa aagaaatggc aataacggcg
     1741 aaacaaacaa ctttggcgac aatatgcaaa ataaccatcc ttttgagcca aactttatgg
     1801 gaagcccttc tcagtttccc gctgacgcaa ctaatatgac atcaatcgac catggcggct
     1861 tcacaaatgt tgacattaca tcaactgaga acaatactac cggtgacaat ggagtggatg
     1921 cgctatcaaa tctactacat aggacaacac acacaccgaa ccgctcctcc cccctaagca
     1981 atgtcacttc tgctcaaaat tcctcttcac aacaacgaaa acattcggaa agcaaagtcg
     2041 atagtaacag cgataataac agctccaaca aagcccccaa tataactgtt cctgactatt
     2101 caattatacc aacctctgtt ttagtaactc tattaccgag ggtcaacgtg cccaacggcg
     2161 catataactc gttgatcagc gcgggatttg acaatgatca aatagatgct atagccgcaa
     2221 taatggcgta tcatcatcaa aaaaagatta gggaaaataa cagtaataat aataaaaaca
     2281 tcaacaccaa tgacagtcaa gaggcaccca ttctaaaaaa catcaacgaa cttttaagtg
     2341 tcttaatacc accctctccg gctgaaactc gtgggccaac taccttatca acgtcgcctt
     2401 cgttcaatga gcacggtgta gtagcagagg cttcttttct aagctccatt ttggaactgg
     2461 gcataaagca tccaaaaagt aataatattc acaatcaacg acaaccttca cgaaacgatc
     2521 ataaaatatc aagagagagt gacggtaaca atggaaacga taatgtccat cataataacg
     2581 ctgttattaa gtcaagtacg acgcgtggag acgaaattgc caagatacga tccgagccaa
     2641 ctttaaatgc aagttcttct gatcacaagg aaaatagttt aaaaagatca cactccggag
     2701 atttgaaaaa taaaaaagta cccgtcgacc gcaagtattc tgataatgaa gacgatgaat
     2761 atgacgatgc agatttacac ggctttgaaa agaagcaact gatcaagaaa gagttagggg
     2821 acgacgatga agatttattg atacagtcga aaaaatctca tcaaaaaaaa aaactaaagg
     2881 aaaaggagtt agaatcatcg atacatgaac tgaccgaaat tgcagcatcc ttacaaaaac
     2941 ggatacatac gttagaaacg gaaaacaagc ttttaaagaa tttagttctg agtagcggtg
     3001 aaacggaagg aataaaaaaa gctgaaagct taaagaagca aatttttgag aaggttcaga
     3061 aagaataaaa taacagacta tacagtttaa ttatatatat atatatatat acgtgcattt
     3121 ttcttaaaga aatataactt ttttcttgta ctgcctgcaa tctctattct tcattcatca
     3181 cacatctatt caaacgcgtt aaaatttttt tgttagccta taagtaaaac gcgtcgcgtg
     3241 tatccaattt tactgaatta aattctttac tcattggatg atatatatct tcttggtcta
     3301 gacaacgaac acataccaat tcagactcac gagaaaaaaa actgtcttca ctctgctatt
     3361 agaaaccaaa tagtatcaag gcataaaata aacagtggaa aatgtcttct aaaagtgaaa
     3421 aattagaaaa attgagaaag ctgcaagccg ctcgaaatgg tacatccatc gatgattac
//