From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Escherichia coli helicase (dnaB) gene, 5' end, and quinone
Date: 29 Sep 92 10:02:59 GMT
Distribution: bionet
Checksum: 57056     5

LOCUS       ECODNABA     2325 bp ds-DNA             BCT       29-SEP-1992
DEFINITION  Escherichia coli helicase (dnaB) gene, 5' end, and quinone
            oxidoreductase (qor) gene, complete cds.
ACCESSION   L02312
KEYWORDS    dnaB gene; helicase; qor gene; quinone oxidoreductase.
SOURCE      Escherichia coli (strain K-12) DNA.
  ORGANISM  Escherichia coli
            Prokaryotae; Gram-negative facultatively anaerobic rods;
            Enterobacteriaceae.
REFERENCE   1  (sites)
  AUTHORS   Nakayama,N., Arai,N., Bond,M.W., Kaziro,Y. and Arai,K.
  TITLE     Nucleotide sequence of dnaB and the primary structure of the dnaB
            protein from Escherichia coli
  JOURNAL   J. Biol. Chem. 259, 97-101 (1984)
  STANDARD  full staff_review
REFERENCE   2  (bases 1 to 2325)
  AUTHORS   Dixon,N.E. and Lilley,P.E.
  TITLE     ..E. coli homolog of zeta crystallin.
  JOURNAL   Unpublished (1992)
  STANDARD  full staff_entry
FEATURES             Location/Qualifiers
     CDS             complement(<1..470)
                     /product="helicase"
                     /gene="dnaB"
                     /codon_start=1
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDL"
                     /citation=[1]
     CDS             553..1536
                     /product="quinone oxidoreductase"
                     /gene="qor"
                     /evidence=EXPERIMENTAL
                     /codon_start=1
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDRVVYAQSALGAYSSVHNII
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREEDLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
                     /citation=[2]
BASE COUNT      603 a    603 c    588 g    531 t
ORIGIN
        1 agatcttcgc tggtacgccc ctgcggatca aaaccagctt cggcaatctc attcgcaacc
       61 gagatcatct cacggacaac ggcacgttca cgcacgatgt ccgcataggc actgatgtta
      121 gccgcacttg gcgtattttt tgacagctct gccagataag caaaaccacc gacgctatcg
      181 agttgcccct ggcgttccag cgattccgca agagtaatca gatcgatagg gctaccgctt
      241 tcctgcaaac gcgccatttc agtaaagata tgacggtgtg ggcgggtgta aaaatcgtct
      301 gctaccacac gctcggctac atcatcccag cgttcgttat ctagcattaa accgcccaac
      361 accgactgct ccgcttcgat cgagtgcgga ggcactttca gcccggcaac ttgtggatcg
      421 cgttcgcggg gttcagcctg ctgtttgttg aagggtttat ttcctgccat agtgaatgga
      481 gttaccgaga taaagaatgg gtcgaaactt tatcatatga agcagaccct gacgatacgt
      541 tctggaggac acatggcaac acgaattgaa tttcacaagc acggtggccc ggaagtactt
      601 caagccgtag agttcactcc tgccgatccg gcggagaatg aaatccaggt cgaaaataaa
      661 gccatcggca tcaattttat cgacacatat atccgcagcg gcctttaccc gccgccatcg
      721 ctacccagcg gattaggcac cgaagcagca ggcatcgtga gtaaagtcgg cagtggtgta
      781 aagcatatta aggcaggtga tcgtgtagtc tatgcgcagt cggcgttagg cgcttacagc
      841 tctgtgcata acattattgc ggataaagcg gcgattctgc ctgcggcaat ttcttttgag
      901 caagctgcgg catccttcct gaaaggctta acggtttatt atctgctgcg caaaacctat
      961 gaaattaaac ccgatgaaca gttcctgttc cacgcagcgg ctggcggcgt tggcttaatt
     1021 gcctgccagt gggcaaaagc cctgggcgcg aaacttatcg gcaccgtagg aaccgcgcaa
     1081 aaagcgcaga gcgcgctaaa agcgggcgcg tggcaggtta ttaactatcg tgaagaggat
     1141 ctggtcgagc ggttaaaaga gatcaccggc ggtaagaaag tgcgcgtggt gtacgattcc
     1201 gtgggcagag acacctggga acggtcgctg gattgcctgc aacgccgcgg cttaatggtc
     1261 agttttggca actcatcagg tgcggttacc ggtgtgaact taggcattct caatcaaaaa
     1321 ggctcgttgt atgtgacacg cccttccctg caaggctata tcaccacgcg ggaggaatta
     1381 accgaggcca gtaatgaact gttctctttg attgccagcg gtgtgattaa ggtcgatgtc
     1441 gccgagcagc agaaatatcc gctgaaggat gcgcagcgtg cgcatgagat tctggaaagc
     1501 cgggcgacgc aaggttccag cctgctgatt ccataaaaga aatagggctt ccacctggga
     1561 agccctttct ttttatagtt cggctgtatg tagggtacag cacgatgaat ctgttagagg
     1621 cgcaatagtg acagatttga ttatcaattc ctattttgtt ctaaggataa aaccttaggt
     1681 tgtgatcatc cgcacaatcc cttagtaacg ccagcggtca taacgctgat atttcggcac
     1741 ttttggtgct ttaatcgcct taataaccca caccaccgca atcgccagta gtaaccacgg
     1801 cagcaactta atcatcaatg ccagcatacc gccgaggaac ataatggccg tcgccacaac
     1861 cagcgcggcg ataatgccca gcaacgaaac gccggtgacc atcagcatga caaaaaagcc
     1921 aatcacaaaa agtagttcca gcatgatgct ctcccaaata tgaaatctct tgctggcatt
     1981 acaagaatca tgccaaaaat aatctattga tttaacagca aaacgccccg cgacggtgcg
     2041 cagggcgtgg tgaatttgac tactttttgg tgaaaagtta acgcttatcc gccaccagtt
     2101 tgagcgcgtg ttccagcaca ttaatgtctg cacccgcttt atgggcattt tcacttaaat
     2161 aacgccgcca ctgccgcgcg ccaggaatac cctggaacaa gcccaacata tgccgggtaa
     2221 tatggccgag atacgtcccc tggctgagtt cacgctcaat gtacggatac atggcgcgca
     2281 ctaccgccac cggatcggca tcggtatccg aggaaccaaa gatct
//