From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: L.esculentum gene for polyphenol oxidase.
Date: 25 Jul 92 10:07:06 GMT
Distribution: bionet
Checksum: 06177     6

LOCUS       TOMPOOXF     2174 bp ds-DNA             PLN       25-JUL-1992
DEFINITION  L.esculentum gene for polyphenol oxidase.
ACCESSION   Z12838
KEYWORDS    polyphenol oxidase.
SOURCE      Lycopersicon esculentum DNA.
  ORGANISM  Lycopersicon esculentum
            Eukaryota; Plantae; Embryobionta; Magnoliophyta; Magnoliopsida;
            Asteridae; Solanales; Solanaceae.
REFERENCE   1  (bases 1 to 2174)
  AUTHORS   Newman,S.M.
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
REFERENCE   2  (bases 1 to 2174)
  AUTHORS   Newman,S.M., Eannetta,N.T., Yu,H., Prince,J.P., de Vicente,C.,
            Tanksley,S.D. and Steffens,J.C.
  TITLE     Organization of the tomato polyphenol oxidase gene family
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
COMMENT     *source: strain=cv. VFNT cherry; *source: clone_library=Charon 35
            library of R. Fischer; *source: chromosome=8; *source:
            is_macronuclear=N; *source: is_proviral=N; *source: is_germline=N;
            From EMBL    entry LEPOOXF;  dated 26-JUN-1992.
FEATURES             Location/Qualifiers
     CAAT_signal     45..48
     TATA_signal     100..104
     CDS             151..2004
                     /product="polyphenol oxidase precursor"
                     /codon_start=1
                     /translation="MSSSTPNTLPLLSTNKSLSSPFTNNHSTFLSKPSQPFLHGRRCQ
                     SFKVSCNVGEHDKNLDAVDRRNVLLGLGGFYGAANLAPLASAAPIPPPDLKSCGVAHI
                     DDKGTEVSYSCCPPVPDDIDSVPYYKFPPMTKLRIRPPAHAADEEYVAKYQLATSRMR
                     ELDKDPFDPLGFKQQANIHCAYCNGAYKIGGKELQVHFSWLFFPFHRWYLYFYERILG
                     SLINDPTFALPYWNWDHPKGMRIPPMFDREGSSLYDEKRNQNHRNGTIIDLGHFGKDV
                     RTPQLQIMTNNLTLMYRQMVTNAPCPSQFFGAPLGSDPEPGMGTIENIPHTPVHIWTG
                     DSPRQGHGEDMGNFYSAGLDPLFYCHHANVDRMWNEWKLIGGKRRDLSNKDWLNSEFF
                     FYDENRNPYRVKVRDCLDSKKMGFDYPPMPTPWRNFKPIRRSSSGKVNTASIAPVSKV
                     FPLAKLDRAISFSLTRPASSRTTQEKNEQQEILTFNKMAYDDTKYVRFDVFLNVDKTV
                     NAEELDKAEFAGSYTSLPHVHGNNDNHVKDVTFTLAITELLEDIGLEDEDTIAVTLVP
                     KVGGEGVSIESVEIKLDLKLGIYLDLDLYLDLKLDLNFILMLNIILILTSS"
     transit_peptide 151..408
     mat_peptide     409..2001
                     /product="polyphenol oxidase"
     misc_binding    739..849
                     /bound_moiety="copper, tyrosinase-like CuA bindingsite"
     misc_binding    1126..1290
                     /bound_moiety="copper, tyrosinase-like CuB bindingsite"
BASE COUNT      602 a    441 c    441 g    690 t
ORIGIN
        1 ttatcgaact aattttgtat tttttttctt ctcaagaacg gatccaatta tatttcatgt
       61 tttacacgtt ttgtgctaca ttaccttcac gattttttgt ataaataaag gtagcaaccc
      121 ttgcacaaag atcactcata acacaataca atgtcttctt ctactcctaa tactcttcct
      181 ttattatcca ccaacaaatc cctctcttct cccttcacca acaaccactc tactttctta
      241 tcaaaaccct ctcaaccttt cctccatgga aggcgctgtc aaagtttcaa ggtttcatgc
      301 aacgttggcg agcatgacaa aaaccttgac gctgttgata ggagaaatgt tcttttaggg
      361 ttaggaggtt tttatggtgc agctaatctt gcaccattag cctctgctgc tcctatacca
      421 ccacctgatc tcaaatcttg tggtgttgcc catatagatg ataaaggtac tgaagtctca
      481 tacagttgtt gccctcctgt acctgatgat atcgatagcg ttccgtacta caagttccct
      541 cctatgacga aactccgcat ccgcccccct gctcatgcgg cggatgagga gtacgtagcc
      601 aagtatcaat tggctacaag tcgaatgagg gaacttgata aagacccttt tgaccctctt
      661 gggtttaaac aacaagctaa tattcattgt gcttattgta acggtgctta taaaattggt
      721 ggtaaagaat tgcaagttca tttctcgtgg cttttctttc cgttccatag atggtacttg
      781 tacttttacg aaaggatatt ggggtcactt attaatgatc caacttttgc tttaccttac
      841 tggaattggg atcatccaaa aggtatgcgt atacctccca tgtttgatcg tgagggatca
      901 tctctttacg atgagaaacg taaccaaaat catcgcaatg gaactattat tgatcttggt
      961 cattttggta aggacgtcag aacacctcag ctacagataa tgactaataa tttaacacta
     1021 atgtaccgtc aaatggttac taatgctcct tgcccgtctc aattcttcgg tgctcctctg
     1081 ggttctgatc cagaaccagg catgggtact attgagaata ttcctcatac accggtccat
     1141 atctggaccg gtgatagtcc tagacaagga cacggtgaag acatgggtaa tttctattca
     1201 gctggtttag acccgctttt ctactgtcac catgctaatg tggacaggat gtggaacgag
     1261 tggaaattaa ttggcgggaa aagaagggat ttatcaaata aagattggtt gaactctgaa
     1321 ttctttttct acgatgaaaa tcgtaaccct taccgcgtga aagtccgtga ttgtttggat
     1381 agtaaaaaaa tgggattcga ttacccgcca atgcccactc catggcgtaa tttcaaacca
     1441 atcagaaggt catcatcagg aaaagtgaat acagcgtcaa ttgcaccagt tagcaaggtg
     1501 ttcccactgg ccaagctgga ccgtgcgatt tcgttctcgc tcacgcggcc agcttcgtca
     1561 aggacaacac aagagaaaaa tgagcagcag gagattctga cattcaataa aatggcatat
     1621 gatgatacga agtatgtaag gttcgatgtg tttttgaacg tggacaagac tgtgaatgca
     1681 gaagaacttg ataaggcgga gtttgcgggg agttatacta gcttgcctca tgttcatgga
     1741 aataatgata atcatgttaa ggatgttact tttacgctgg cgataactga actgttggag
     1801 gatattggat tggaagatga agatacaatt gcggtaactt tggttccaaa agttggtggt
     1861 gaaggtgtat ccattgaaag tgtggagatc aagcttgatc ttaaacttgg gatttatctt
     1921 gatcttgatc tctatcttga tcttaaactt gacttgaact ttatcttgat gttgaacatt
     1981 atcttaatct tgacttctag ttgaatccaa ggatttcgaa cttgatcttg aatccagcag
     2041 tcttgatctt tatcgttctt gatcttgatg gttcttgaaa ttttaatgct tgaattcttg
     2101 aacttgtaga gaaatctgca gtttttgatc cacgagcttt ctcgagcttc ttgttaaaag
     2161 tcttggagcc tata
//