From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: D.pseudoobscura Adh gene for alcohol dehydrogenase
Date: 28 Dec 92 21:26:29 GMT
Distribution: bionet
Checksum: 40637 8

LOCUS       DROADHGN13   3483 bp ds-DNA             INV       01-DEC-1992
DEFINITION  D.pseudoobscura Adh gene for alcohol dehydrogenase
ACCESSION   X62193
KEYWORDS    ADH gene; alcohol dehydrogenase.
SOURCE      Drosophila pseudoobscura DNA.
  ORGANISM  Drosophila pseudoobscura
            Eukaryota; Animalia; Metazoa; Arthropoda; Uniramia; Insecta;
            Pterygota; Neoptera; Holometabola; Diptera; Brachycera;
            Cyclorrhapha; Schizophora; Drosophiloidea; Drosophilidae.
REFERENCE   1  (bases 1 to 3483)
  AUTHORS   Schaeffer,S.W. and Miller,E.L.
  TITLE     Estimates of gene flow in Drosophila pseudoobscura determined from
            nucleotide sequence analysis of the alcohol dehydrogenase region
  JOURNAL   Genetics 132, 471-480 (1992)
  STANDARD  full automatic
COMMENT     *source: strain=GB109; *source: isolate=Gundlach Bundschu Winery,
            Sonoma Valley, *source: California; *source: chromosome=IV section
            88;
               From EMBL    entry DPADHGN13;  dated 16-OCT-1992.
FEATURES             Location/Qualifiers
     mRNA            join(40..119,915..1020,1084..1488,1549..1964)
                     /note="adult mRNA (Adh)"
     mRNA            join(882..1020,1084..1488,1549..1964)
                     /note="larval mRNA (Adh)"
     promoter        8..14
                     /note="adult promoter (Adh)"
     prim_transcript 40..1964
                     /note="adult primary transcript (Adh)"
     exon            40..119
                     /note="adult exon 1a"
     intron          120..914
                     /note="adult intron"
     promoter        846..853
                     /note="larval promoter (Adh)"
     prim_transcript 882..1964
                     /note="larval primary transcript (Adh)"
     exon            882..1020
                     /note="larval exon 1"
     exon            915..1020
                     /note="adult exon 1b"
     intron          1021..1083
                     /number=1
     exon            1084..1488
                     /number=2
     intron          1489..1548
                     /number=2
     exon            1549..1964
                     /number=3
     polyA_signal    1941..1946
     polyA_site      1964
     CDS             join(928..1020,1084..1488,1549..1815)
                     /EC_number="1.1.1.1"
                     /gene="Adh"
                     /product="alcoholdehydrogenase"
                     /codon_start=1
                     /translation="MSLTNKNVVFVAGLGGIGLDTSRELVKRNLKNLVILDRIDNPAA
                     IAELKAINPKVTITFYPYDVTVPVAETTKLLKTIFAQVKTIDVLINGAGILDDHQIER
                     TIAVNYTGLVNTTTAILDFWDKRKGGPGGIICNIGSVTGFNAIYQVPVYSGSKAAVVN
                     FTSSLAKLAPITGVTAYTVNPGITKTTLVHKFNSWLDVEPRVAEKLLEHPTQTSQQCA
                     ENFVKAIELNKNGAIWKLDLGTLEPITWTQHWDSGI"
BASE COUNT      977 a    797 c    796 g    913 t
ORIGIN      
        1 cgtagtgtat ttaaggcact gcacatgtcg aggaccacga ttattgtcta cagtgcagtc
       61 agtagttgtc aggtgcagca ggcggaccag catttcttta aatctactca atcgatcaag
      121 taagtaactg taatccaagt acagtcggcc catcccactc ggttctgccg taactcaaga
      181 aatttcttga tagcgcagtt ccagttgtaa aacgtcgttt aaaaaaacat ctcgttggag
      241 cttgggttga aaaacgttcg agttgggcgt agaaaagctc aataataaaa tatcaaaaaa
      301 gagaaaatat cttcccaaag cagctgtcca ttttttgtta aagtgataga tcaaaaattc
      361 gtatgacgta tttgccaagt gctctttatg caaataattt cctcaattct aattctacaa
      421 gaagcacttt tggcttttgt ccagaaaaaa caatttgttt acacttgtcg gatgataaca
      481 gaagcggacc gacggctaag taacataaca gtcgcctgtt aagtaatgcc ctcactcgct
      541 gttagatggc cgcgcgtgtc gactccgata gaccagcaga cacgcagtag ttttcctcaa
      601 gaacctgaac tctaaacata gacataattt acgatacctt gagactcaaa taaaaataca
      661 tgtatgagtg gtattctatt cctgattaga ctcgcacaca cacacgtacg agtattaaca
      721 taaatttgcc ggcaaataac caaaacaaaa cgaacgcaaa cgatccgaac acgtaatgcg
      781 agagataaga aacagaaaag ctcgacgtga aagaagcttc tggcggaagc tcttgccgaa
      841 ctccctataa atacgagaca gaaaaccaga ataaatataa taacaaactg ttgaattgtc
      901 ccatcgattc ccagatcaaa agacagaatg tctctcacca acaagaacgt cgttttcgtg
      961 gccggtctgg gcggcattgg cctggacacc agccgggagt tggtcaagcg taatctgaag
     1021 gtaagagaga acgaattcca tggaatctat ggaatccaaa atttaaaaat taatttattt
     1081 tagaacctgg tcatcctgga tcgcattgac aatccggctg ccattgccga actgaaggca
     1141 atcaatccca aggtgaccat caccttctat ccctacgatg tgactgtgcc cgtcgctgag
     1201 accaccaagc tcctgaagac catctttgcc caggtgaaga caatcgatgt cctgatcaac
     1261 ggtgctggca tcctggacga tcatcagatc gagcgcacca ttgccgttaa ctacacgggc
     1321 ctggtcaaca ccaccacagc cattctggac ttctgggaca agcgcaaggg cggcccaggc
     1381 ggcatcattt gcaacattgg ctccgtcacc ggtttcaatg ccatctacca ggtgcccgtt
     1441 tactccggct ccaaggcggc ggtggttaac tttacctcct ccctggcggt aagtaatcca
     1501 acgtttttcc tactccccaa aactaaatat ttctttgaaa tcccatagaa actggctccc
     1561 attactggtg tcactgctta cactgtcaat cctggcatca ctaagaccac tctggtccac
     1621 aaattcaact cgtggctgga tgtggagccc cgtgtggcgg agaagctgct cgagcatccc
     1681 acccagacct ctcagcagtg cgccgagaac tttgtgaagg ccatcgagct gaacaagaac
     1741 ggtgccatct ggaagttgga tttgggcacc ttggagccca tcacatggac ccagcactgg
     1801 gactcgggca tctaaaccat cccagagact ctatgggaca tggcgtttta gcttttagtt
     1861 tctttttttc cactcaattg ttacgtatat acatacacat atggcaataa ggctgatttg
     1921 aaccccgttt tgaatatgat aatacaaaat tatatttgag aaatttcaac aaaatcgata
     1981 agagctatag ctctctttta attaaaaaaa actagataaa aagagcaatg gtcagtggtg
     2041 gtcagtggtc gttgtggcct ctatcgattt ccacacaaaa actttactgt ttagtaatag
     2101 aaaagaactt gagaaaggca gccaaaagaa tgtacgatct gacgggtaag catgtctgct
     2161 atgtggctga ctgcggtggc attgcactgg agaccagcaa ggttctcatg accaagaata
     2221 tagcggtgag tggtagagtg gtgtagagag ttgagagtgt tccgaagtgg ttctggttct
     2281 taaacaagtc tctagtctct agtctctagt ctcttggctg ctgctggtag ctgtcgaaac
     2341 taattaagtt aatgactttt tcatgttttg tttttgtccg ctgacttttg ctgttcaaag
     2401 ctacaattaa gtttatgttt tgagtttgat ctacttttgc ttaagggaag tactttcgat
     2461 tacggattgc tggcatataa aaatagatac caaggaatgt gcatcatatc atattgattc
     2521 tatcttgatt ctttgcccag aaactggcca tccttcagag cgtggaaaac cccccggcca
     2581 tcgctcagtt acaatcgatt aagcacagca cacagatctt cttctggacc ttcgatgtga
     2641 ccatggcccg cgaggagatg aagaagtact tcgatgaggt catggtccag atggactaca
     2701 tcgatgtact gatcaatggg gccaccctgt gcgatgagcg gaacattgat gccaccatca
     2761 atacgaatct gaccggcatg atgaacaccg tggccactgt gctgccctat atggaccgaa
     2821 agatgggcgg ttcgggtgga ctgattgtga atgtcacctc cgtcatagga ctggatccat
     2881 cgccggtctt ttgtgcgtac agtgcctcaa agtttggtgt gattgggttc acccgaagtc
     2941 tagcggtgag tgggcgagat cttagcttca aaatgctctc taatgtcttc cattcttccg
     3001 caggatcccc tgtattacac ccaaaatggt gtggctgtca tggccgtctg ctgtggtccc
     3061 accaaagtgt ttgtcgatcg cgaactgact gccttcctgg aatacggcca aacctttgcc
     3121 gatcgcctgc gtcgtgcccc ctgccaatcg acggccgtct gcggccagaa tatagtaaat
     3181 gccatcgaga gatcggagaa cggacagatc tggattgccg acaagggcgg cctggagtcg
     3241 gttgccctcc actggtattg gcatatggcc gatcagtttg tgaactacat gcagagcact
     3301 gacgatgagg atcaggagtt ttttttgggc cagcgatgag gcatgccttg atccacggaa
     3361 taaaacatca aatgtgtcac aaaaagtagg caacttgttt gtgggtctgg aaatagattt
     3421 gaaatatttt gaggtcatat gaataatgac ataaaagagg gtgcctgaaa gtatgccaag
     3481 ttt
//