From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Escherichia coli guaC gene for guanosine 5'-monophosphate
Date: 22 Dec 92 22:51:19 GMT
Distribution: bionet
Checksum: 46682 8

LOCUS       ECOQUAC      1991 bp ds-DNA             BCT       30-NOV-1992
DEFINITION  Escherichia coli guaC gene for guanosine 5'-monophosphate
            oxidoreductase (EC 1.6.6.8)
ACCESSION   X07917 M33020
KEYWORDS    GMP reductase; guaC gene;
            guanosine 5'-monophosphate oxidoreductase; oxidoreductase;
            reductase.
SOURCE      Escherichia coli DNA.
  ORGANISM  Escherichia coli
            Prokaryotae; Gram-negative facultatively anaerobic rods;
            Enterobacteriaceae.
REFERENCE   1  (bases 1 to 1991; 1 to 1991)
  AUTHORS   Andrews,S.C. and Guest,J.R.
  TITLE     Nucleotide sequence of the gene encoding the GMP reductase of
            Escherichia coli K12
  JOURNAL   Biochem. J. 255, 35-43 (1988)
  STANDARD  full automatic
COMMENT     SWISS-PROT; P15344; GUAC$ECOLI.
               *source: strain=K12; clone=pGS89; **map: map position=2.6 min;
               From EMBL 27   entry ECQUAC;  dated 06-DEC-1990.
FEATURES             Location/Qualifiers
     -35_signal      25..30
                     /note="-35 region promoter 2"
     repeat_unit     44..49
                     /note="inverted repeat A"
     -10_signal      50..55
                     /note="-10 region promoter 2"
     precursor_RNA   61..>61
                     /note="transcript from promoter 2"
     repeat_unit     62..67
                     /note="inverted repeat A'"
     -35_signal      98..103
                     /note="-35 region promoter 1"
     repeat_unit     119..130
                     /note="inverted repeat B"
     -10_signal      122..127
                     /note="-10 region promoter 1"
     -35_signal      126..131
                     /note="-35 region promoter 3"
     misc_feature    128..134
                     /note="discriminator sequence promoter 1"
     repeat_unit     132..147
                     /note="inverted repeat B'"
     precursor_RNA   135..>135
                     /note="transcript from promoter 1"
     -10_signal      148..153
                     /note="-10 region promoter 3"
     misc_feature    155..161
                     /note="discriminator sequence promoter 3"
     precursor_RNA   160..>160
                     /note="transcript from promoter 3"
     RBS             198..201
                     /note="ribosome binding site"
     repeat_unit     285..291
                     /note="inverted repeat C"
     repeat_unit     301..307
                     /note="inverted repeat C'"
     repeat_unit     620..627
                     /note="inverted repeat D"
     repeat_unit     644..651
                     /note="inverted repeat D'"
     repeat_unit     908..915
                     /note="inverted repeat E"
     repeat_unit     923..928
                     /note="inverted repeat F"
     repeat_unit     932..939
                     /note="inverted repeat E'"
     repeat_unit     933..938
                     /note="inverted repeat F'"
     repeat_unit     979..987
                     /note="inverted repeat G"
     repeat_unit     997..1005
                     /note="inverted repeat G'"
     repeat_unit     1050..1055
                     /note="inverted repeat H"
     repeat_unit     1064..1069
                     /note="inverted repeat H'"
     terminator      1257..1286
                     /note="pot. transcriptional terminator; putative"
     repeat_unit     1324..1329
                     /note="inverted repeat J"
     repeat_unit     1335..1340
                     /note="inverted repeat J'"
     repeat_unit     1569..1577
                     /note="inverted repeat K"
     repeat_unit     1598..1606
                     /note="inverted repeat K'"
     repeat_unit     1710..1715
                     /note="inverted repeat L"
     repeat_unit     1733..1738
                     /note="inverted repeat L'"
     repeat_unit     1794..1802
                     /note="inverted repeat M"
     repeat_unit     1810..1818
                     /note="inverted repeat M'"
     CDS             210..1250
                     /note="GMP reductase;  (AA 1-346)"
                     /codon_start=1
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHYSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFARADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMKR
                     HVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFIR
                     VQEQENRIFNNL"
BASE COUNT      493 a    494 c    508 g    496 t
ORIGIN      
        1 gaattcatca tgattatcaa aacgttaaaa atgagtgcac gaaagcgaaa ttgatgaaac
       61 gttcgctcac tatttaccag gtaaatttat gggattgtag cgtaaaaaaa gacaatttcg
      121 cagtcttgcg ccgcattgat tagtgcgtat gatagcgtca ctggagttgc gctcttaccc
      181 ttatagccat taaccccagg aatccgcaca tgcgtattga agaagatctg aagttaggtt
      241 ttaaagacgt tctcatccgc cctaaacgct ccactcttaa aagccgttcc gatgttgaac
      301 tggaacgtca attcaccttc aaacattcag gtcagagctg gtccggcgtg ccgattatcg
      361 ccgcaaatat ggacaccgta ggcacatttt ctatggcctc tgcgctggct tcttttgata
      421 ttttgactgc tgtgcataaa cactattctg tcgaagagtg gcaagcgttt atcaacaatt
      481 cttccgctga tgtgctgaaa catgtgatgg tttctaccgg tacgtctgat gcggatttcg
      541 aaaaaactaa acagattctc gacctgaacc cggcattaaa cttcgtttgt attgacgtgg
      601 cgaatggtta ttccgaacac ttcgtgcagt tcgttgcgaa agcgcgtgaa gcgtggccga
      661 ccaaaaccat ttgtgctggt aacgtagtga ctggtgaaat gtgtgaggag cttatcctct
      721 caggtgccga tatcgttaaa gttggcattg gcccaggttc tgtttgtaca actcgcgtca
      781 aaacaggcgt cggttatccg caactttctg cggtaatcga atgtgccgat gctgcgcacg
      841 gtctgggcgg aatgatcgtc agcgatggtg gctgcaccac gccgggcgat gtggcgaaag
      901 cctttgcgcg tgccgatttc gtcatgcttg gcggcatgct ggcgggccac gaagagagcg
      961 gcggtcgcat cgttgaggag aacggcgaga aatttatgct gttctacggc atgagctccg
     1021 agtctgcgat gaaacgtcac gttggcggcg ttgcggaata tcgcgcagca gaaggtaaaa
     1081 ccgttaagct gccgctgcga ggcccggttg aaaataccgc gcgagatatt ttgggcggcc
     1141 tgcgttcagc ttgtacatac gttggggctt cacgcctgaa agagctgacc aagcgcacca
     1201 cgtttattcg tgtgcaggaa caagaaaacc gcatcttcaa caacctgtaa tctcccaacg
     1261 ctggcgtgga gcaacacgcc acggttatcc catcccactc atcgcatcgc ctaaatggaa
     1321 aattggcaga tacattgcca ccaccagcgt accaataatt cctcccgtta tgatcagcaa
     1381 cgcggttcag taaggctgcg aggttatccg ccagcgccat tgtgttttcc cgatgatgat
     1441 gggcgaggtt gtctaacatg agatccagag agccggatgc ctctcctgtt ctcactaatt
     1501 gcaaacagag cgggctaaac tcaccggtat tttttagcgc cagccagatg ggttgaccgt
     1561 tactgatatc gtgctggatt tgtgtcagaa gttgcaccca gtacgggcag cgcattgttt
     1621 ctctgacgct ctctacgccc tgtaaaaaag taatgcctgc actttgtgtc agcgccagaa
     1681 tcgtaaagat ctgcgtgagt ttttgtcccc gcatcagtga acccataatc gggatgcgta
     1741 acagcaattt ctgccgcact ataagccagg tcggtcggcg catcagcaac ttattggcta
     1801 tcgccagcag aaagccgaac acaccagcag ccagctccat tcgccactaa agtctgccag
     1861 cgtcatgatc ccctgcgtta gtgccggtag tggggtgttg aaggtcttat agatagcggc
     1921 aaactccggc agacacaaaa tgcagcattg ccacaaccac catgattagc catcgctaaa
     1981 atgatgatgg g
//