From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Clostridium thermaceticum large subunit corrinoid/iron-sulfur
Date: 22 Dec 92 22:50:53 GMT
Distribution: bionet
Checksum: 26019 5

LOCUS       CLOMTPA      1625 bp ds-DNA             BCT       30-NOV-1992
DEFINITION  Clostridium thermaceticum large subunit corrinoid/iron-sulfur
            protein gene, complete cds.
ACCESSION   L07099
KEYWORDS    large subunit corrinoid/iron-sulfur protein;
            methyl transfer protein.
SOURCE      Clostridium thermoaceticum (strain DMS 521) (library: Ragsdale)
            DNA.
  ORGANISM  Clostridium thermoaceticum
            Prokaryotae; Firmicutes; Endospore-forming rods and cocci;
            Bacillaceae.
REFERENCE   1  (bases 1 to 1625)
  AUTHORS   Lu,W.-P., Schiau,I., Cunningham,J.R. and Ragsdale,S.W.
  TITLE     Sequence and expression of the gene encoding the
            corrinoid/iron-sulfur protein from Clostridium thermoaceticum and
            reconstitution of the recombinant protein to full activity
  JOURNAL   J. Biol. Chem. (1992) In press
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     CDS             100..1440
                     /function="methyl transfer protein"
                     /evidence="EXPERIMENTAL"
                     /note="this is the corrinoid containing subunit of the
                     C/Fe-SP involved in transfer of methyl from
                     methyl-H4folate to carbon monoxide dehydrogenase in CO2
                     fixation by anaerobic bacteria"
                     /product="large subunit corrinoid/iron-sulfur protein"
                     /codon_start=1
                     /translation="MPLTGLEIYKQLPKKNCGECGTPTCLAFAMNLASGKASLDSCPY
                     VSDAAREALDAAAAPPIAKVVLGAGPTAVEMGDETELFRHDKRFYHETAIAIQVSDNL
                     SSEELKAKVEAINGLNFDRVGQHYTIQAIAIRHDADDPAAFKAAVASVAAATQLNLVL
                     MADDPDVLKEALAGVADRKPLLYAATGANYEAMTALAKENNCPLAVYGNGLEELAELV
                     DKIVALGHKQLVLDPGARETSRAIADFTQIRRLAIKKRFRSFGYPIIALTTAANPLDE
                     VLQAVNYVTKYASLVVLRTDAKEHLLPLLSWRQNLYTDPQVPIRVEEKLNEIGAVNEN
                     SPVYVTTNFSLTYYSVEGEIESTKIPSYLLSVDTDGLSVLTAYADGKFEAEKIAAVMK
                     KVDLDNKVKRHRIIIPGAVAVLKGKLEDLTGWEVIVGPREASGIVAFARANLAS"
BASE COUNT      381 a    474 c    441 g    329 t
ORIGIN      
        1 atcccattat gtgagagcct gcacccggtt ccggggctat cccggggatt ggccccggaa
       61 ccgagctgaa ttaatcatga aagaaaggag tcaacaaata tgcctttgac gggactggag
      121 atttacaagc agctacccaa aaagaattgt ggcgagtgcg ggacacccac ctgtctggcc
      181 ttcgccatga acctggcctc cggaaaggcc agccttgatt cctgtccgta tgtttcagat
      241 gccgcccggg aggccctgga cgcggccgcg gcaccaccca ttgccaaggt agtcctgggc
      301 gccgggccga ctgccgtaga aatgggggat gagacggaac tcttccgcca tgataaacgt
      361 ttttaccatg aaaccgccat tgccatccag gttagcgaca acttgagcag tgaagaactg
      421 aaggctaaag tcgaagctat aaatggcctg aacttcgacc gggtgggcca gcactacacc
      481 atccaggcca tagccatccg ccatgatgcc gatgaccctg ctgctttcaa ggcagcggta
      541 gccagtgtag ccgccgctac ccagttaaac cttgtcctta tggccgatga tcctgacgta
      601 ttaaaggaag ccctagcagg agtagccgac cgcaagcccc tcttatatgc cgccaccggc
      661 gctaattacg aagccatgac cgccctggcc aaagaaaaca actgccccct ggccgtctat
      721 ggtaacggtc tggaggaact ggccgaactg gtagataaaa tcgttgccct gggccacaag
      781 cagttggtcc tcgatcccgg tgccagggag acctccaggg ccatcgcgga tttcacccag
      841 atccgccgcc tggccattaa gaaacgtttc cgttccttcg gttatcccat tatcgccctt
      901 actactgctg ccaatccatt agacgaggta ctccaggcag ttaactatgt gaccaagtat
      961 gctagcttgg tggttttacg caccgatgcc aaagaacacc tgctccccct cttgtcctgg
     1021 cgccagaacc tctacaccga cccccaggtt cccatcaggg tagaggagaa actgaatgaa
     1081 atcggtgccg tcaacgagaa ttcgccggtc tacgtaacca ccaacttctc cctgacctat
     1141 tactccgtcg agggcgagat cgagagcacc aagatcccca gttacctgct ctcggtggat
     1201 accgacggac tgtcagtctt gacggcctat gccgatggta aatttgaagc cgagaaaatc
     1261 gccgccgtta tgaaaaaggt ggacctggac aataaggtta aacgccaccg gatcattatt
     1321 cccggggctg tcgccgtcct gaagggcaaa ctggaagact taactggatg ggaagttatc
     1381 gttggcccca gggaagccag cggcatcgtg gcctttgccc gggccaacct ggcttcatag
     1441 gataaacggg aggggatgcc tgtggatcag tttgctgtca cctttttacc tgataatatc
     1501 accgtccggg tggcggctgg taccagcatt atggaggcgg ccaaccaggc cggcctgccc
     1561 ctgaaaagca cctgcggcgg ggccggcacc tgtggtcgct gtgccatcaa ggtccaggag
     1621 gggaa
//