HDTenEightyP/NetNews huggingface dataset:
From: mulligan@kean.ucs.mun.ca (Martin E. Mulligan)
Newsgroups: bionet.molbio.methds-reagnts
Subject: Re: Which salt of X-P to use for detection of alkaline phosphatase?
Date: 12 Nov 92 20:14:16 GMT
Distribution: bionet
Organization: Memorial University of Newfoundland

In article <1992Nov12.160826.25460@menudo.uh.edu>, bchs1b@Elroy.UH.EDU (Michael Benedik) writes:
> In article <01GR2UC9PTO2934TI6@INVERMAY.CRI.NZ>, VANNESTEJ%RUA1@INVERMAY.CRI.NZ writes:
>>
>>
>>	Hello,
>>
>>
>>	Which salt of X-P to use for detection of alkaline phosphatase?
>>
....stuff deleted
>>
> 
> We use the toluidine salt in my lab which works just fine. I don't know
> if it really matters or not, but this does work. Lots of places sell
> it, including sigma, biorad and numerous others. Genetics folks call
> it XP but immunology folks call it BCIP used for detection of
> western blots etc with alkakline phosphatase conjugated antibodies. 
> BCIP and XP are one and the same...
> 
> 
> ----------------------------------------------------------------------
>  Michael Benedik				INTERNET: Benedik@uh.edu
>  Dept. of Biochemical & Biophysical Sciences	
>  University of Houston				BITNET: Benedik@uhou
> -----------------------------------------------------------------------

Once, we inadvertently ordered the Na salt from Boehringer instead
of the toluidine salt.  While I cannot tell you anything about its
relative effectiveness, we did find that the Na salt was completely
insoluble in dimethylformamide.  That tipped us off that something was
wrong.  However, we just ordered the correct salt rather than fiddle
around with the Na salt.

Martin E. Mulligan
Dept. of Biochemistry
Memorial University of Newfoundland
From: kristoff@net.bio.net (David Kristofferson)
Newsgroups: bionet.molbio.methds-reagnts
Subject: Re: Frequently Asked Questions (FAQ) List
Keywords: FAQ list
Date: 11 Nov 92 23:12:14 GMT
Distribution: bionet
Organization: BIOSCI International Newsgroups for Biology

The Methods FAQ will be available for FTP shortly in the METHODS
newsgroup archive on net.bio.net in pub/BIOSCI/METHDS-REAGNTS.

				Sincerely,

				Dave Kristofferson
				BIOSCI/bionet Manager

				kristoff@net.bio.net
From: award@vax.path.ox.ac.uk
Newsgroups: bionet.molbio.methds-reagnts
Subject: luciferase antibody
Date: 10 Nov 92 11:32:26 GMT
Organization: Oxford University Molecular Biology Data Centre
Nntp-Posting-User: award

Does anyone have, or know of, a source of antibody specific to firefly 
luciferase? If so, please let me know.

Thankyou,

Dr. Andrew Ward
CRC Growth Factors (Zoology) Oxford
Dept. of Zoology
Oxford University
South Parks Rd
Oxford
UK

Tel: 0865 27122  Fax: 0865 310447
From: muriana@aclcb.purdue.edu (Peter M. Muriana)
Newsgroups: bionet.molbio.methds-reagnts
Subject: Re: sequencing from 300-500 bp
Date: 12 Nov 92 20:50:29 GMT
Reply-To: muriana@aclcb.purdue.edu
Organization: Purdue University AIDS Center

In article <Af0KlOu00iV005CmZv@andrew.cmu.edu>, "Morris F. Manolson" <mm6y+@andrew.cmu.edu> writes:
>Dear Netters,  I am sure someone must have asked this before but......
>
>I am trying to sequence off of one reaction 300 to 500 bps using the 
>standard IBI or BRL 44 cm glates.  Without having to resort to ultra-long
>plates (of which I don't have), does anyone have any suggesting on how to
>improve the resolution of the bands in the 300 to 500 bp region.  My reactions
>are fine and I can see bands up to 600 bp from the primer, I just can't
>resolve them well enough to read.  I am using 6% acrylamide wedge gels and run
>them for 24 hours at 900 Volts, this gives me nice sequence on average
>up to 350 bp,  on a great gel I can get 400. Thanks in advance, Morrie 

Morrie,

I used to run double sets of lanes in which the second set (from the same
sequencing RXN) was not applied to the sequencing gel until the first set
of lanes had been electrophoresed for some time.  It takes a little trial 
and error to minimize the overlap of the two sets of lanes.
 - Peter
*********************************************************************
*  Peter M. Muriana, Ph.D.		Phone	= (317)-494-8284    *
*  Dept. of Food Science		FAX	= (317)-494-7953    *
*  Purdue University		E-mail	= muriana@aclcb.purdue.edu  *
*  W. Lafayette, IN  47907                                          *
*********************************************************************
From: VANNESTEJ%RUA1@INVERMAY.CRI.NZ
Newsgroups: bionet.molbio.methds-reagnts
Subject: Which salt of X-P to use for detection of alkaline phosphatase?
Date: 12 Nov 92 09:25:10 GMT
Distribution: bionet



	Hello,


	Which salt of X-P to use for detection of alkaline phosphatase?

	To know if the gene I cloned contains a signal peptides sequence, I mutagenized 
the gene with a transposon that carries a phoA gene derivative that lacks signal 
peptides sequence.  The next step is the detection (or the non detection) of the 
alkaline phosphatase, this is easily done (according to the publications) by 
incorporing in the medium the 5-bromo-4-chloro-3indolyl-phosphate,also called X-P 
or X-phosphate.  In the catalogue of Boehringer Mannheim (the only distributor of 
X-P I found) there is two salts of X-P that are listed; a disodium salt and a 
4-toluidine salt. The local representatives could not tell me which salt I should 
use.  Can anybody help me?  Could you please also let me know if you know of 
another source of the compound than Boehringer Mannheim.

	Thank you,

	Joel


------------------------------------------------------------------------------
  Joel L. Vanneste
  The Horticulture and Food Research Institute of New Zealand Ltd 
  Ruakura Agricultural Centre,		
  Private Bag 3123, Hamilton,		e mail: VANNESTEJ@RUAKURA.CRI.NZ  
  New Zealand						fax 64 7 838 5073
------------------------------------------------------------------------------
From: WAGNERF@BRUNB.BITNET (Wagner Fontes)
Newsgroups: bionet.molbio.methds-reagnts
Subject: RE: HPLC Query
Date: 3 Nov 92 20:36:48 GMT
Distribution: bionet


Hi!
   I was thinking about your problem, but you have many
variables to consider before choosing one instrument.
   First: you must define exactly the purposes for it,
that means kind of samples, kind of separations (preparative
or analytical), the precision you need, etc.
   Then comes another problem : how much do you want to
spend buying this instrument.
   Here we are working with seven HPLC instruments, each
one performing specific tasks. The brands are ABI, Waters
and LKB. I would be glad to help you, but I need more info
about your problem.

Wagner Fontes
Brazilian Center of Protein Sequencing
Biochemistry / Protein Chemistry Laboratory
University of Brasilia - Brazil


.
Newsgroups: bionet.molbio.methds-reagnts
From: pedykema@iastate.edu (Philip E Dykema)
Subject: Re: Western Blot staining
Keywords: Western Blot Staining
Organization: Iowa State University, Ames, IA
Distribution: bionet
Date: Thu, 12 Nov 1992 04:47:30 GMT

In article <9211112154.AA15755@nmr1.pt.Cyanamid.COM> sandy@pt.Cyanamid.COM (Sandy Silverman) writes:
>We once read about a method to detect protein on a Western blot prior
>to antibody detection.  It involved a red dye and acetic acid.  It was 
>reversible.  Does anyone have the reference?   Thanks-----Sandy

Sambrook et. al on page 18.67 (Vol 3) outline a method using Ponceau S 
(which can be obtained from Sigma).  The formula is a follows:
2 g Ponceau S
30 g trichloroacetic acid
30 g sulfosalicyclic acid
water to 100 ml
I highly recommend beginning with less than 20 ml water as the two acids bring
with embibed water.  The stain works great and reverses with a normal TS
(Tris/saline) wash.
Good Luck.

___________
Philip Dykema   (pedykema@vincent.iastate.edu)
Botany Department
Iowa State University
From: vaidyaa@HAL.HAHNEMANN.EDU
Newsgroups: bionet.molbio.methds-reagnts
Subject: YAC libraries of the rat genome
Date: 5 Nov 92 21:57:20 GMT
Distribution: bionet

Dear Colleagues:
	I am posting this a second (and ,for the time being, the last) time
asking to see if a YAC library of the rat genome was available in any
laboratory.  I am posting this on behalf of a colleague who would welcome even
rumors about the existence of such a library.  Please respond if you have any
information about this.

	Thank you for reading this message.

				Akhil Vaidya
From: deustachio@mcclb0.med.nyu.edu
Newsgroups: bionet.molbio.evolution
Subject: Re: RAPD list?
Date: 10 Nov 92 21:47:50 GMT
Organization: NYU Medical Center, New York, NY 10016, USA

> In article <1992Nov6.141029.17085@gserv1.dl.ac.uk> , ZANDE@RUGR86.RUG.nl
> writes:
>>You can subscribe to a RAPD newsgroup by sending the command
>>
>>subscribe RAPD-L <your name> to listserv@buyvm
>>
> 
> Are you sure this is the correct address?  When I try to subscribe, my
> mail is sent back because the host "buyvm" doesn't exist.  
> 
> Kathie Hodge
> kh11@cornell.edu

That's a bitnet address, not internet.  Also, isn't it byuvm, not buyvm?
From: bishopj@botany.washington.edu
Newsgroups: bionet.molbio.evolution
Subject: rapd mailing list
Date: 11 Nov 92 18:40:26 GMT
Organization: University of Washington

To subscribe to the RAPD mailing list, send a message to 
listserv@byuvm.bitnet, with the message containing the line 
SUB RAPD-L Your Name
To find out about other options send a message with the line
HELP

--

John Bishop
Department of Botany
University of Washington, Seattle
bishopj@botany.washington.edu
From: ZANDE@RUGR86.RUG.nl
Newsgroups: bionet.molbio.evolution
Subject: interpretation RAPD data
Date: 6 Nov 92 13:54:00 GMT
Distribution: bionet
X-Envelope-To: mol-evol@daresbury.AC.UK
Content-Identifier: interpretatio...
X-Vms-Cc: ZANDE
Original-To: mol-evol@uk.ac.daresbury
X-Vms-To: IN%"mol-evol@daresbury.ac.uk"


Hi there too

The problem is not whether the bands are faint, but if they are reproducible!
So always run replicate reactions so there can be no mistake about fragments.
If the fragments are too faint, try incorporating biotin (dUTP) during the last ten rounds od amplification. Visualisation after blotting of the pattern with
AMPPD (chemoluminiscence kits are available from many sources) will confirm
the faintest fragments. If fragments are reproducible, yes they should be scored. 

As for differences within and between populations, it is best to try to
identify "signature fragments" for each population. Ideally these are fragments present as a monomorphic band in one population and absent in any other.
Another, more basic problem seems to be: how is a RAPD locus defined?
Is it just the stretch of DNA between two primer sites, is it just one primer site, both primer sites? Only when a good definition of a RAPD locus is available
there is a possibility of meaningful quantitative comparison of species.

You can subscribe to a RAPD newsgroup by sending the command

subscribe RAPD-L <your name> to listserv@buyvm

I have not yet heard of any computer programs for RAPD data, possibly due to the above described troublesome interpretation of RAPD loci.

Good luck with your data-set.

Louis v.d. Zande 
Dept. Genetics
Univ. Groningen
The Netherlands
Newsgroups: bionet.molbio.evolution
From: lur@jester.usask.ca (Rui Lu)
Subject: How to interpret RAPD data?
Organization: University of Saskatchewan
Distribution: bionet.molbio.evolution
Date: Fri, 6 Nov 1992 02:23:34 GMT

Hi, there,

      I am currently doing RAPD analysis in area of population genetics.
As the data accumulating, I start to worry about how to interpret the result.
The problems are:

      1). How to score the very faint bands? Sometimes I can hardly tell
whether there is a band or not. Should I consider the kind of bands are the 
same as other strong bands?

      2). How can I deal with the variation within one population when I try
to find difference between populations(e.g. species-specific bands)? How many
individuals can be considered as representative of a population?

      3). Is there anyone know any computer programs that can be used for
RAPD analysis? Please send me a copy or tell me where to download them.

      4). I can obscurely remember there was a message about how to subscribe
to a RAPD directory. Can someone tell me how please?

      If anyone have met similar problem like these, I very much appreciate
his(her) discussion on this.
-- 
Ray Lu
lur@sask.usask.ca
Ph:(306)966-4440/4442
Newsgroups: bionet.molbio.evolution
From: lfiddick@nyx.cs.du.edu (larry fiddick)
Subject: behavioral ecology, evolution and evolutionary ecology
Organization: University of Denver, Dept. of Math & Comp. Sci.
Date: Fri, 13 Nov 92 02:18:47 GMT


are there any newsgroups or mailing lists devoted to behavioral ecology,
evolved behavior and evolutionary ecology in general?

Newsgroups: bionet.molbio.evolution
From: Your Name Here <NetID@cornell.edu>
Subject: Re: RAPD list?
X-Xxdate: Tue, 10 Nov 92 13: 12:38 GMT
Nntp-Posting-User: kh11@cornell.edu
Organization: Cornell University
X-Useragent: Nuntius v1.1.1d7
Date: Tue, 10 Nov 1992 18:10:42 GMT

In article <1992Nov6.141029.17085@gserv1.dl.ac.uk> , ZANDE@RUGR86.RUG.nl
writes:
>You can subscribe to a RAPD newsgroup by sending the command
>
>subscribe RAPD-L <your name> to listserv@buyvm
>

Are you sure this is the correct address?  When I try to subscribe, my
mail is sent back because the host "buyvm" doesn't exist.  

Kathie Hodge
kh11@cornell.edu
Newsgroups: bionet.molbio.evolution
From: Your Name Here <NetID@cornell.edu>
Subject: Re: RAPD list?
X-Xxdate: Tue, 10 Nov 92 13: 14:35 GMT
Nntp-Posting-User: kh11@cornell.edu
Organization: Cornell University
X-Useragent: Nuntius v1.1.1d7
Date: Tue, 10 Nov 1992 18:12:12 GMT

In article <1992Nov6.141029.17085@gserv1.dl.ac.uk> , ZANDE@RUGR86.RUG.nl
writes:
>You can subscribe to a RAPD newsgroup by sending the command
>
>subscribe RAPD-L <your name> to listserv@buyvm
>

Are you sure this is the correct address?  When I try to subscribe, my
mail is sent back because the host "buyvm" doesn't exist.  

Kathie Hodge
kh11@cornell.edu
From: toby@carson.u.washington.edu (Toby Bradshaw)
Newsgroups: bionet.molbio.evolution
Subject: Re:  Kosambi centimorgans
Date: 5 Nov 92 17:03:53 GMT
Organization: University of Washington, Seattle

In article <1992Nov3.183314.7159@mcclb0.med.nyu.edu> deustachio@mcclb0.med.nyu.edu writes:
>Nigel Walker asked:
>> What is a Kosambi centimorgan?  How does it differ from a plain vanilla
>> centimorgan?  Is there a difference? 
>and Joe Felsenstein replied:
>>
>> I think a Kosambi centimorgan is just a name for an ordinary centimorgan,
>> when that centimorgan is estimated from recombination fractions, using
>> the mapping function of Kosambi:
>>
>>    Kosambi, D. D.  1944. The estimation of map distance from recombination
>>    values.  Annals of Eugenics  12: 172-175.
>>
>> There are other mapping functions, and there is no real reason to append the
>> name of the mapping function to the name "centimorgan" as if what it
>> calculates is incommensurable with ordinary centimorgans.
>>
>It's not quite that simple.  Mapping functions embody theories about inter-
>ference (interactions - positive or negative - among a given number of
>progeny chromosomes into a 'true' (contingent on the validity of your theory)
>map distance.  Different mapping functions make quite different assumptions
>in this respect and, especially for low density maps (i.e., recombination
>fractions greater than 10% between adjacent markers), yield appreciably
>different results.  At the same time, all the mapping functions I know of
>converge as the distance between adjacent markers gets small. 
>
>Peter D'Eustachio
>NYU Medical Center - Biochemistry
>peter@mcbcm2.med.nyu.edu

I'm guessing that the creator of the "Felsenstein mapping function"
(Felsenstein [1979] A mathematically tractable family of of genetic
mapping functions with different amounts of interference. Genetics
91:769-775) is pretty well aware of the theory and practice of
genetic mapping, and of the underlying assumptions.  I think Joe's
point is that all of the mapping functions give the "true" map
distance if their assumptions are met; proving that the assumptions
are (or are not) met is the problem.

Toby Bradshaw
Department of Biochemistry and College of Forest Resources
University of Washington, Seattle
toby@u.washington.edu
From: dstothar@magnus.acs.ohio-state.edu (Diane Stothard)
Newsgroups: bionet.molbio.evolution
Subject: Re: How to interpret RAPD data?
Date: 12 Nov 92 01:02:43 GMT
Distribution: bionet.molbio.evolution
Organization: The Ohio State University

I attended a meeting this past weekend where the hot topic of conversation was 
exactly your question. We all came to the conclusion that, since RAPD data are 
so inconsistent (even using different machines will give you different 
results!) it is best to decide what experimental conditions you want to use 
(temp, MgCl2, etc) and stick with those conditions for all your RAPDs. If a 
certain set of RAPDs does not respond to these conditions, eliminate them from 
further analysis. As far as scoring faint bands, I think that may also be an 
arbitrary decision on your part. Good luck!
From: francis@monod.Biol.McGill.CA (Francis Ouellette)
Newsgroups: bionet.molbio.embldatabank
Subject: Re: new e-address for EMBL?
Date: 14 Nov 92 04:58:59 GMT
Organization: McGill University

alistair@sun.bdc.ubc.ca (Alistair Blachford) writes:

>I'd like to ask for an electronic order form from the folks at EMBL.
>The address I have tried, "datalib@embl.bitnet", does not work.  Is
>there a new address?  Please respond to me directly at:
>		alistair@bcu.ubc.ca

Dear Alistair,

here are the addresses for EMBL (with cc to you directly!)


 EMBL File Server (e-mail requests)       NetServ@EMBL-Heidelberg.DE
 FASTA e-mail server                        FASTA@EMBL-Heidelberg.DE
 Quicksearch e-mail server                  Quick@EMBL-Heidelberg.DE
 Anonymous FTP                                FTP.EMBL-Heidelberg.DE

 Problems, feedback (human contact)        NetHelp@EMBL-Heidelberg.DE
 EMBL Data Library enquiries               DataLib@EMBL-Heidelberg.DE
 EMBL Data Library sequence submissions   DataSubs@EMBL-Heidelberg.DE
 Software submissions and problems        Software@EMBL-Heidelberg.DE     


regards,

francis


--
| B.F. Francis Ouellette  
| manager, yeast chromosome I project
| dept of biology, McGill university, Montreal, Qc, Canada
| francis@monod.biol.mcgill.ca
From: alistair@sun.bdc.ubc.ca (Alistair Blachford)
Newsgroups: bionet.molbio.embldatabank
Subject: new e-address for EMBL?
Date: 13 Nov 92 21:51:51 GMT
Organization: Zoology, University of B.C., Vancouver, B.C., Canada
NNTP-Posting-Host: sun.bcu.ubc.ca

I'd like to ask for an electronic order form from the folks at EMBL.
The address I have tried, "datalib@embl.bitnet", does not work.  Is
there a new address?  Please respond to me directly at:
		alistair@bcu.ubc.ca
Thanks,
	Alistair
-- 
Alistair Blachford
Biosciences Computing Unit         |    Email: alistair@bcu.ubc.ca
University of British Columbia     |    Phone: 604-822-2645
Vancouver, Canada, V6T 1W5         |    Fax:   604-822-2416
From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human immunodeficiency virus type 1 (10304.D.31) RNA.
Date: 5 Nov 92 23:07:30 GMT
Distribution: bionet
Checksum: 14113 1

LOCUS       HIV304D31X     56 bp ss-RNA             VRL       22-SEP-1992
DEFINITION  Human immunodeficiency virus type 1 (10304.D.31) RNA.
ACCESSION   L03435
KEYWORDS    .
SOURCE      Human immunodeficiency virus type 1 RNA.
  ORGANISM  Human immunodeficiency virus type 1
            Viridae; ss-RNA enveloped viruses; Positive strand RNA virus;
            Retroviridae; Lentivirinae.
REFERENCE   1  (bases 1 to 56)
  AUTHORS   McNearney,T., Hornickova,Z., Markham,R., Birdwell,A., Arens,M.,
            Saah,A. and Ratner,L.
  TITLE     Relationship of human immunodeficiency virus type 1 sequence
            heterogeneity to stage of disease
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. (1992) In press
  STANDARD  full automatic
BASE COUNT       20 a      8 c     13 g     15 t
ORIGIN      
        1 ctgttaaatg gcagtctagc agaagaagag gtagtaatta gatctgacaa tttcac
//
From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: acfA-acfD intergenic region: acfD=accessory colonization factor
Date: 6 Nov 92 18:02:32 GMT
Distribution: bionet
Checksum: 46341 3

LOCUS       S110311       400 bp    DNA             NLM       21-OCT-1992
DEFINITION  acfA-acfD intergenic region: acfD=accessory colonization factor
            synthesis, acfA=accessory colonization factor synthesis [Vibrio
            cholerae, El Tor O395, Genomic, 400 nt]
ACCESSION   S41756
KEYWORDS    .
SOURCE      Vibrio cholerae El Tor O395
  ORGANISM  Vibrio cholerae
            Unclassified.
REFERENCE   1  (bases 1 to 400)
  AUTHORS   Parsot,C. and Mekalanos,J.J.
  TITLE     Structural analysis of the acfA and acfD genes of Vibrio cholerae:
            effects of DNA topology and transcriptional activators on
            expression.
  JOURNAL   J. Bacteriol. 174, 5211-5218 (1992)
  STANDARD  full automatic
COMMENT     This entry incorporates information provided by the National
            Library of Medicine's GenInfo Backbone as NCBI gibbsq 110311.
            This sequence comes from Fig. 3.
            Region: acfA-acfD intergenic region.
FEATURES             Location/Qualifiers
     CDS             complement(1..160)
                     /partial
                     /note="accessory colonization factor synthesis;  For the
                     protein sequence (NCBI gibbsq 110312):  This sequence
                     comes from Fig. 3."
                     /gene="acfD"
                     /product="AcfD"
                     /codon_start=1
                     /translation="MKIRIVSLIVLGFLIGCKHESIITPTVPADGSGGNALNPGLVGG
                     YLPDIGVRI"
     CDS             334..400
                     /partial
                     /note="accessory colonization factor synthesis;  For the
                     protein sequence (NCBI gibbsq 110313):  This sequence
                     comes from Fig. 3."
                     /gene="acfA"
                     /product="AcfA"
                     /codon_start=1
                     /translation="MQKTLSAIFLFTTLSANAAPYI"
BASE COUNT      172 a     68 c     50 g    110 t
ORIGIN      
        1 ggatccgaac accaatatct ggcaaataac cacctaccaa accaggattt agtgcgttac
       61 cacccgaacc atcagcaggc acagtagggg taattatact ttcatgctta caacctataa
      121 gaaaacccaa aactatcaat gatacaatac gaatcttcat cttaagccta ttaaacaaaa
      181 aacaagaatt aattatcctt gttaaaaatg aaaaaaatcg ctttatacat tttgttaaat
      241 ttatttttaa aaatttaacg agttagcata aaaaaaaata aaaaattaaa gttagaataa
      301 aatgtgaaac acaggtaaaa ataggagtaa aaaatgcaaa aaactttatc agcaattttt
      361 ttgtttacga ctttatcagc aaatgcagca ccgtatattg
//
From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: {Tst1 element homolog} [Solanum tuberosum=potatoes, line AM80/5793,
Date: 5 Nov 92 23:02:38 GMT
Distribution: bionet
Checksum: 22585 2

LOCUS       M177392S2      20 bp    DNA             NLM       22-OCT-1992
DEFINITION  {Tst1 element homolog} [Solanum tuberosum=potatoes, line AM80/5793,
            Genomic, 20 nt, segment 2 of 2] 
ACCESSION   S76102
KEYWORDS    .
SEGMENT     2 of 2
SOURCE      potatoes line AM80/5793
  ORGANISM  Solanum tuberosum
            Unclassified.
REFERENCE   1  (bases 1 to 20)
  AUTHORS   Kikuchi,S., Liu,X.J., Frommer,W.B., Koster-Topfer,M. and
            Willmitzer,L.
  TITLE     Identification and structural characterization of further DNA
            elements in the potato and pepper genomes homologous to the
            transposable element-like insertion Tst1.
  JOURNAL   Mol. Gen. Genet. 230, 494-498 (1991)
  STANDARD  full automatic
COMMENT     This entry incorporates information provided by the National
            Library of Medicine's GenInfo Backbone as NCBI gibbsq 76102.
            This sequence comes from Figure 3.A.
BASE COUNT        4 a      3 c      7 g      6 t
ORIGIN      
        1 atacgccttt ggattgaggg
//