From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: L.esculentum gene for polyphenol oxidase.
Date: 25 Jul 92 10:07:05 GMT
Distribution: bionet
Checksum: 33159     6

LOCUS       TOMPOOXE     2074 bp ds-DNA             PLN       25-JUL-1992
DEFINITION  L.esculentum gene for polyphenol oxidase.
ACCESSION   Z12837
KEYWORDS    polyphenol oxidase.
SOURCE      Lycopersicon esculentum DNA.
  ORGANISM  Lycopersicon esculentum
            Eukaryota; Plantae; Embryobionta; Magnoliophyta; Magnoliopsida;
            Asteridae; Solanales; Solanaceae.
REFERENCE   1  (bases 1 to 2074)
  AUTHORS   Newman,S.M.
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
REFERENCE   2  (bases 1 to 2073)
  AUTHORS   Newman,S.M., Eannetta,N.T., Yu,H., Prince,J.P., de Vicente,C.,
            Tanksley,S.D. and Steffens,J.C.
  TITLE     Organization of the tomato polyphenol oxidase gene family
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
COMMENT     *source: strain=cv. VFNT cherry; *source: clone_library=Charon 35
            library of R. Fischer; *source: chromosome=8; *source:
            is_macronuclear=N; *source: is_proviral=N; *source: is_germline=N;
            From EMBL    entry LEPOOXE;  dated 26-JUN-1992.
FEATURES             Location/Qualifiers
     CAAT_signal     43..46
     TATA_signal     104..108
     CDS             151..1914
                     /product="polyphenol oxidase precursor"
                     /codon_start=1
                     /translation="MSSSSSITTTLPLCTNKSLSSSFTTTNSSLLSKPSQLFLHGRRN
                     QSFKVSCNANNVDKNPDAVDRRNVLLGLGGLYGAANLAPLATAAPIPPPDLKSCGTAH
                     VKEGVDVIYSCCPPVPDDIDSVPYYKFPSMTKLRIRPPAHAADEEYVAKYQLATSRMR
                     ELDKDPFDPLGFKQQANIHCAYCNGAYKVGGKELQVHFSWLFFPFHRWYLYFYERILG
                     SLINDPTFALPYWNWDHPKGMRIPPMFDREGSSLYDEKRNQNHRNGTIIDLGHFGKEV
                     DTPQLQIMTNNLTLMYRQMVTNAPCPSQFFGAAYPLGSEPSPGQGTIENIPHTPVHIW
                     TGDKPRQKNGEDMGNFYSAGLDPIFYCHHANVDRMWNEWKLIGGKRRDLTDKDWLNSE
                     FFFYDENRNPYRVKVRDCLDSKKMGFDYAPMPTPWRNFKPIRKSSSGKVNTASIAPVS
                     KVFPLAKLDRAISFSITRPASSRTTQEKNEQEEILTFNKISYDDRNYVRFDVFLNVDK
                     TVNADELDKAEFAGSYTSLPHVHGSNTNHVTSLTFKLAITELLEDIGLEDEDTIAVTL
                     VPKAGGEEVSIESVEIKLEDC"
     transit_peptide 151..411
     mat_peptide     412..1911
                     /product="polyphenol oxidase"
     misc_binding    739..849
                     /bound_moiety="copper, tyrosinase-like CuA bindingsite"
     misc_binding    1132..1296
                     /bound_moiety="copper, tyrosinase-like CuB bindingsite"
BASE COUNT      594 a    423 c    433 g    624 t
ORIGIN
        1 attgtgggga gagagtgagt aaatactcca agataagatc tacaattatc agcaacgtgt
       61 tacacatttt gtgctacaat ataccttcac cattttgtgt atatataaag gttgcatctc
      121 ttcaaacaaa aatcactcca tcacaacaca atgtcttctt cttcttctat tactactact
      181 cttcctttat gcaccaacaa atccctctct tcttccttca ccaccaccaa ctcatccttg
      241 ttatcaaaac cctctcaact tttcctccac ggaaggcgta atcaaagttt caaggtttca
      301 tgcaacgcaa acaacgttga caaaaaccct gacgctgttg atagacgaaa cgttctttta
      361 gggttaggag gtctttatgg tgcagctaat cttgcaccat tagcgactgc tgcacctata
      421 ccacctcctg atctcaagtc ttgtggtact gcccatgtaa aagaaggtgt tgatgtaata
      481 tacagttgtt gccctcctgt acccgatgat atcgatagtg ttccgtacta caagttccct
      541 tctatgacta aactccgcat ccgcccccct gctcatgcgg cggatgagga gtacgtagcc
      601 aagtatcaat tggctacgag tcgaatgagg gaacttgata aagacccctt tgaccctctt
      661 ggctttaaac aacaagctaa tattcattgt gcttattgca acggtgctta caaagttggt
      721 ggtaaagaat tgcaagttca tttctcgtgg cttttctttc cctttcatag atggtacttg
      781 tacttttacg aaagaatttt gggatcactt attaatgatc caacttttgc tttaccttac
      841 tggaattggg atcatccaaa aggcatgcgt atacctccca tgtttgatcg tgagggatca
      901 tctctttacg atgagaaacg taaccaaaat catcgcaatg gaactattat tgatcttggt
      961 cattttggta aggaagttga cacacctcag ctacagataa tgactaataa tttaacccta
     1021 atgtaccgtc aaatggttac taatgctcct tgcccttccc aattcttcgg tgctgcttac
     1081 cctctgggtt ctgaaccaag tccgggtcag ggtactattg aaaacatccc tcatactccg
     1141 gttcacatct ggaccggtga caaacctcgt caaaaaaacg gtgaagacat gggtaatttc
     1201 tactcagccg gtttagatcc gattttttac tgccaccatg ccaatgtgga caggatgtgg
     1261 aatgaatgga aattaattgg cgggaaaaga agggatttaa cagataaaga ttggttgaac
     1321 tctgaattct ttttctacga tgaaaatcgt aacccttacc gtgtgaaagt ccgtgactgt
     1381 ttggacagta aaaaaatggg attcgattac gcgccaatgc ccactccatg gcgtaatttt
     1441 aaaccaatca gaaagtcatc atcaggaaaa gtgaatacag cgtcaattgc accagttagc
     1501 aaggtgttcc cattggcgaa gctggaccgt gcgatttcgt tctctatcac gcggccagcc
     1561 tcgtcaagga caacacaaga gaaaaatgag caagaggaga tactgacatt caataaaata
     1621 tcgtatgatg ataggaacta tgtaaggttc gatgtgtttc tgaacgtgga caagactgtg
     1681 aatgcagatg agcttgataa ggcggagttt gcagggagtt atactagctt gccgcatgtt
     1741 catggaagta atactaatca tgttaccagt cttactttca agctggcgat aactgaactg
     1801 ttggaggata ttggattgga agatgaagat actattgcgg tgactttggt tccaaaagct
     1861 ggcggtgaag aagtgtccat tgaaagtgtg gagatcaagc ttgaggattg ttaaagtctg
     1921 catgagttgg tggctatggt gccaaatttt atgtttaatt agtataatta tgtgtggttt
     1981 gagttatgtt ttatgttaaa atgtatcagc tcgatcgata gctgattgct agttgtgtta
     2041 atgctatgta tgaaataaat aaatggttgt cttc
//