HDTenEightyP/NetNews huggingface dataset:
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: H.francisci rearranged immunoglobulin heavy chain (Vh-Dh-Jh)
Date: 15 Aug 92 10:01:56 GMT
Distribution: bionet
Checksum: 26009     2

LOCUS       HEFRIGHJ      357 bp ss-RNA             VRT       15-AUG-1992
DEFINITION  H.francisci rearranged immunoglobulin heavy chain (Vh-Dh-Jh)
ACCESSION   Z11789
KEYWORDS    Ig D-segment; heavy chain; immunoglobulin; joining region;
            variable region.
SOURCE      Heterodontus francisci RNA.
  ORGANISM  Heterodontus francisci
            Eukaryota; Animalia; Chordata; Vertebrata; Chondrichthyes;
            Elasmobranchii; Euselachii; Neoselachii; Galeomorphii;
            Heterodontiformes; Heterodontidae.
REFERENCE   1  (bases 1 to 357)
  AUTHORS   Litman,G.
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
REFERENCE   2  (sites)
  AUTHORS   Hinds-Frey,K.R., Nishikata,H., Litman,R.T. and Litman,G.W.
  TITLE     Multiple somatic mechanisms are employed to generate immunoglobulin
            heavy chain diversity in a primitive vertebrate
  JOURNAL   J. Exp. Med. (1992) In press
  STANDARD  full staff_review
COMMENT
            From EMBL    entry HSRIGHJ;  dated 06-AUG-1992.
FEATURES             Location/Qualifiers
     CDS             1..357
                     /partial
                     /product="Ig heavy chain (Vh-Dh-Jh)"
                     /codon_start=1
                     /translation="DVVLTQPEADTGKPKGSLRLTCKTSGFSLGSYYMTWVRQGPGQG
                     LEWLVSYHSSSYQYYAPEIKDRFTASKDTSNNIFALDMKSLKTEDTAMYYCAREDSGG
                     NWGLDYWGQGTMVTVTA"
                     /partial
BASE COUNT       93 a     87 c    105 g     72 t
ORIGIN
        1 gatgtcgtgt tgactcagcc agaagcagac accgggaaac ccaaaggctc cctgagactg
       61 acctgtaaaa ccagcgggtt cagtcttggc agctactata tgacctgggt ccgacagggt
      121 cccgggcagg ggctggagtg gttggttagc taccatagtt catcatacca gtactacgcc
      181 cctgagatta aggatcgatt tactgcgtcc aaagacactt caaacaacat cttcgctttg
      241 gacatgaaga gcctgaagac cgaagacact gccatgtatt actgtgcaag agaggacagc
      301 ggtgggaact gggggctcga ctactgggga caggggacca tggtgacggt gactgca
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: M.musculus pva gene for parvalbumin, promoter region and exon 1
Date: 22 Aug 92 10:01:10 GMT
Distribution: bionet
Checksum: 05434     2

LOCUS       MUSPVAEX1     321 bp ds-DNA             ROD       22-AUG-1992
DEFINITION  M.musculus pva gene for parvalbumin, promoter region and exon 1
ACCESSION   X62826
KEYWORDS    cytosolic calcium binding protein; parvalbumin; pva gene.
SOURCE      Mus musculus DNA.
  ORGANISM  Mus musculus
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Rodentia; Myomorpha; Muridae; Murinae.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Schleef,M.
  JOURNAL   Unpublished (1991)
  STANDARD  simple staff_entry
REFERENCE   2  (sites)
  AUTHORS   Schleef,M., Zuehlke,C.H., Jockusch,H. and Schoeffl,F.
  TITLE     The structure of the mouse parvalbumin gene
  JOURNAL   Mamm. Genome 3, 217-225 (1992)
  STANDARD  full staff_review
COMMENT     *source: strain=ssp. domesticus, strain=A2G; *source: stage=adult;
            *source: chromosome=15;
            From EMBL    entry MMPVAEX1;  dated 01-JUL-1992.
FEATURES             Location/Qualifiers
     CAAT_signal     183..189
     TATA_signal     206..211
     exon            236..301
                     /number=1
                     /gene="pva"
     intron          302..321
                     /number=1
                     /note="5'portion"
BASE COUNT       67 a     82 c    105 g     67 t
ORIGIN
        1 aggtgtgccc tgcttggacc ttagtgctgg gtgctgtagg gaggtagagg ccaagggtta
       61 ttaataggcc tggctcaata ggcctggatg ggggtgaatg tgatacagac actcagcacc
      121 gtggctgggg agtacctgac accggaaggg gagggggcca ggggctgggg gagcgccacc
      181 tcccaaaata gccagggctg tagactattt aagtggctgt cccaccacga gtctcatttc
      241 ccaacaggat ctcccaccag cccagctttt ctgtataggc tccggcctct gctcatccaa
      301 ggtaagcggt agcctctgag g
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human chromosome 4 sequence-tagged site STS4-264
Date: 21 Aug 92 10:08:19 GMT
Distribution: bionet
Checksum: 37961     3

LOCUS       HUM4STS264    426 bp ds-DNA             PRI       21-AUG-1992
DEFINITION  Human chromosome 4 sequence-tagged site STS4-264
ACCESSION   L00768
KEYWORDS    STS; human chromosome 4; sequence tagged site.
SOURCE      Homo sapiens, clone p4-200 from a chromosome 4 plasmid library;
            vector pBluescript II KS + (Stratagene)
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 426)
  AUTHORS   Goold,R.D., diSibio,G., Dugaiczyk,A., Smith,K.A., Xu,H.,
            Magrane,G., Lang,D.B., Cox,D.R., Masters,S.B. and Myers,R.M.
  TITLE     Sequence-Tagged Sites for Human Chromosome 4
  JOURNAL   Unpublished (1992) see COMMENT for author address
  STANDARD  full automatic
COMMENT     PCR components: 25 ng of human genomic DNA, 10 pmol of each
            oligonucleotide, 200 micro-M dNTPs, 0.25 U Taq  polymerase (Cetus)
            in 10 micro-l of 50 mM KCl-20 mM  Tris-HCl, pH 8.3 (at room temp),
            2.5 mM MgCl-2.  Initial denaturation at 94degC for 1.5 min, then 30
            cycles of  94degC for 15 sec, 62degC for 23 sec, and 72degC for 30
            sec, followed by a final extension at 72degC for 3.5 min, using a
            Perkin-Elmer 9600 thermocycler. PCR-amplified product size 241 bp.
            Sequence submitted by:
            Human Genome Mapping Center
            Box 0925
            University of California San Francisco
            San Francisco, CA 94143-0925  USA
            Phone: (415) 502-1612   Fax: (415) 476-8391
            e-mail: hgmcprobes@cgl.ucsf.edu
FEATURES             Location/Qualifiers
     misc_feature    67..90
                     /label=Primer1
     misc_feature    complement(283..307)
                     /label=Primer2
BASE COUNT      117 a     79 c     55 g    165 t     10 others
ORIGIN
        1 gatctgtgca aactaatcat aattttagaa cattttcatc aactcatttt aaaaaagtac
       61 ccttagcaat cactttccat ttctccccac tccctcaacc taagcaaata actaatttac
      121 tttnngnctt cacagatttg nttatactgg acgtgggata taagtagaat catacattac
      181 atggtctttn ggncttgatt ttttcactta acataatgtt tttaaggttc aaacacattg
      241 taacatatat cagtacttca ttccttttta ttgacaaata atgtctcatt gtatggctct
      301 atcatatttt ctttacctat tatatatcca ttccttatca gttcatagac atttgggttg
      361 ttttggggtt gtttccactt nctggntagt ttnnataatg ctgctatgag cattcaggtg
      421 caagtt
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human chromosome 4 sequence-tagged site STS4-173
Date: 21 Aug 92 10:07:16 GMT
Distribution: bionet
Checksum: 27815     3

LOCUS       HUM4STS173    425 bp ds-DNA             PRI       21-AUG-1992
DEFINITION  Human chromosome 4 sequence-tagged site STS4-173
ACCESSION   M95245
KEYWORDS    STS; human chromosome 4; sequence tagged site.
SOURCE      Homo sapiens, clone C4-103 from Los Alamos National Laboratory
            chromosome 4 cosmid library, plate 4-1-1R, pos 1E; vector SuperCos
            1 (Stratagene)
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 425)
  AUTHORS   Goold,R.D., diSibio,G., Dugaiczyk,A., Smith,K.A., Xu,H.,
            Magrane,G., Lang,D.B., Cox,D.R., Masters,S.B. and Myers,R.M.
  TITLE     Sequence-Tagged Sites for Human Chromosome 4
  JOURNAL   Unpublished (1992) see COMMENT for author address
  STANDARD  full automatic
COMMENT     PCR components: 25 ng of human genomic DNA, 10 pmol of each
            oligonucleotide, 200 micro-M dNTPs, 0.25 U Taq  polymerase (Cetus)
            in 10 micro-l of 50 mM KCl-20 mM  Tris-HCl, pH 8.3 (at room temp),
            2.5 mM MgCl-2.  Initial denaturation at 94degC for 1.5 min, then 30
            cycles of  94degC for 15 sec, 62degC for 23 sec, and 72degC for 30
            sec, followed by a final extension at 72degC for 3.5 min, using a
            Perkin-Elmer 9600 thermocycler. PCR-amplified product size 267 bp.
            Sequence submitted by:
            Human Genome Mapping Center
            Box 0925
            University of California San Francisco
            San Francisco, CA 94143-0925  USA
            Phone: (415) 502-1612   Fax: (415) 476-8391
            e-mail: hgmcprobes@cgl.ucsf.edu
FEATURES             Location/Qualifiers
     misc_feature    27..50
                     /label=Primer1
     misc_feature    complement(268..293)
                     /label=Primer2
BASE COUNT      134 a     65 c     78 g    136 t     12 others
ORIGIN
        1 tnttcccttc atggtcaagt ctnaatcagt tgatttaccc aaagaaccag atgatgaaaa
       61 gattaggctg ggtcacctgg catctttnct ttctttgggg tatgtcctgg atgaagacag
      121 aaaatatatc aggtcacctg ctttaacttc acttaaatac atacaccttg tttagaggaa
      181 cttgtgtctt tatggagttt aaacttgttt tttataatag tatattttgt tttgggggga
      241 cactaaagaa gcccattgaa caaagtaggc aatatgctca gattatatac ttgccataac
      301 atgtttacaa natgtattaa aacacattaa gtaaaagcca cntattatat tagttaaata
      361 aaaaccagct aatgtagtat ncnncnggna tggtgagtgn gaccaagatt aggctggtta
      421 aatnt
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: H.sapiens dystrophin gene, exon 62.
Date: 20 Aug 92 10:02:46 GMT
Distribution: bionet
Checksum: 07124     2

LOCUS       HUMDYST03     232 bp ds-DNA             PRI       20-AUG-1992
DEFINITION  H.sapiens dystrophin gene, exon 62.
ACCESSION   M86886
KEYWORDS    Becker muscular dystrophy; Duchenne muscular dystrophy; dystrophin;
            integral membrane glycoprotein; membrane-associated protein.
SOURCE      Homo sapiens DNA.
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 232)
  AUTHORS   Roberts,R.G., Coffey,A.J., Bobrow,M. and Bentley,D.R.
  TITLE     Determination of the exon structure of the distal protion of the
            dystrophin gene by vectorette PCR
  JOURNAL   Genomics 13, 942-950 (1992)
  STANDARD  simple staff_entry
FEATURES             Location/Qualifiers
     exon            96..156
                     /number=62
                     /gene="dystrophin"
BASE COUNT       57 a     47 c     42 g     86 t
ORIGIN      chromosome X, map position Xp21
        1 agtgtaaaac agtaattctg gagattaatg ttgtctttcc tgtttgcgat gaatttgacc
       61 tccttgcctt tctttttttc ctcccttctt ttcagcgtct gtccagggtc cctgggagag
      121 agccatctcg ccaaacaaag tgccctacta tatcaagtaa gttggaagta tcacattttt
      181 aaaagagcat ttattgtgac taacctgtat attcacaagt gagtttattt tt
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human immunodeficiency virus type 2 (FOENVC42) envelope protein
Date: 18 Aug 92 10:02:06 GMT
Distribution: bionet
Checksum: 44943     2

LOCUS       HIVENVC42     453 bp ss-RNA             VRL       18-AUG-1992
DEFINITION  Human immunodeficiency virus type 2 (FOENVC42) envelope protein
            fragment.
ACCESSION   M87092
KEYWORDS    envelope protein.
SOURCE      Human immunodeficiency virus type 2 uncultured blood mononuclear
            cells (3/90 sample) RNA.
  ORGANISM  Human immunodeficiency virus type 2
            Viridae; ss-RNA enveloped viruses; Positive strand RNA virus;
            Retroviridae; Lentivirinae.
REFERENCE   1  (bases 1 to 453)
  AUTHORS   Gao,F., Yue,L., White,A.T., Pappas,P.G., Barchue,J., Hanson,A.P.,
            Greene,B.M., Sharp,P.M., Shaw,G.M. and Hahn,B.H.
  TITLE     Human infection by genetically-diverse SIVsm-related HIV-2 in West
            Africa
  JOURNAL   Nature (1992) In press
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     misc_feature    1..453
                     /note="PCR derived HIV-2 envelope fragment"
                     /gene="env"
                     /note="putative"
                     /citation=[1]
BASE COUNT      180 a     76 c     86 g    111 t
ORIGIN
        1 gacgtggtca agagacaaca aggattgttg caactaaccg tctaggaaac taaaaacctc
       61 cagactaaag tcactgctat cgaaaagtac ctaaaggacc aggcacagct aaattcatgg
      121 ggatgcgcgt tcagacaggt ctgccacacc actgtaaaat ggccaaatag tactctcaca
      181 cctgactgaa acaatatgac ttggcagaag tgagaaagaa aggttaattt cctaaaggca
      241 aatataacac agttgttaga ggaagcacaa attcaacagg agaaaaacat gtataagtta
      301 caaaaactaa ataactgaaa tgtatttagc aactggtttg accttacttc ttaaatcaag
      361 tatatacagt ataaagtctt catagtaata ggtataatat tgttaaaaat agtaatctat
      421 gtagtacaaa tgttagctaa gttaagtaag gga
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human chromosome 4 sequence-tagged site STS4-353
Date: 21 Aug 92 10:09:18 GMT
Distribution: bionet
Checksum: 53111     3

LOCUS       HUM4STS353    435 bp ds-DNA             PRI       21-AUG-1992
DEFINITION  Human chromosome 4 sequence-tagged site STS4-353
ACCESSION   L00811
KEYWORDS    STS; human chromosome 4; sequence tagged site.
SOURCE      Homo sapiens, clone p4-360 from a chromosome 4 plasmid library;
            vector pBluescript II KS + (Stratagene)
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 435)
  AUTHORS   Goold,R.D., diSibio,G., Dugaiczyk,A., Smith,K.A., Xu,H.,
            Magrane,G., Lang,D.B., Cox,D.R., Masters,S.B. and Myers,R.M.
  TITLE     Sequence-Tagged Sites for Human Chromosome 4
  JOURNAL   Unpublished (1992) see COMMENT for author address
  STANDARD  full automatic
COMMENT     PCR components: 25 ng of human genomic DNA, 10 pmol of each
            oligonucleotide, 200 micro-M dNTPs, 0.25 U Taq  polymerase (Cetus)
            in 10 micro-l of 50 mM KCl-20 mM  Tris-HCl, pH 8.3 (at room temp),
            2.5 mM MgCl-2.  Initial denaturation at 94degC for 1.5 min, then 30
            cycles of  94degC for 15 sec, 62degC for 23 sec, and 72degC for 30
            sec, followed by a final extension at 72degC for 3.5 min, using a
            Perkin-Elmer 9600 thermocycler. PCR-amplified product size 226 bp.
            STS sequence contains a simple sequence tandem repeat (CA).
            Sequence submitted by:
            Human Genome Mapping Center
            Box 0925
            University of California San Francisco
            San Francisco, CA 94143-0925  USA
            Phone: (415) 502-1612   Fax: (415) 476-8391
            e-mail: hgmcprobes@cgl.ucsf.edu
FEATURES             Location/Qualifiers
     misc_feature    20..44
                     /label=Primer1
     misc_feature    complement(233..245)
                     /label=Primer2
BASE COUNT      142 a    108 c     71 g    107 t      7 others
ORIGIN
        1 gatcacagca tggtataatc aaatactgtt acccatggtc actttttcct aattgttcag
       61 tggaacccag tctagaaaga tgagattaca ggcagggagg caaactcatg acactaaaaa
      121 tgctccatta taacaagcac tcttctaaac aacacacaca cacacacaca cacacacaca
      181 cacacacaca cacacacaag cacatacatt cctttagttt aagaaaacct gctcaccttg
      241 gagtcttttt ccagtagttg attntgataa aagagaatgc ttctattctt tctgaagact
      301 ttgtgnccag aagagttggn tttctcaaga acaagccana actcagcata ggacttactt
      361 accttaaaat ccagttgang atccactagt tctagagcgg ncgccaccgc ggtggagctc
      421 caattcgncc tatag
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Gallus gallus Ig unproductively rearranged L-chain gene V-J-region,
Date: 21 Aug 92 10:06:01 GMT
Distribution: bionet
Checksum: 59601     3

LOCUS       CHKIGLAZK     435 bp ds-DNA             VRT       21-AUG-1992
DEFINITION  Gallus gallus Ig unproductively rearranged L-chain gene V-J-region,
            partial cds.
ACCESSION   M96975
KEYWORDS    immunoglobulin light chain; joining region; pseudogene;
            variable region.
SOURCE      Gallus gallus (individual_isolate REV-T (CSV), strain SC line
            (Hyline International, Dallas Center, Iowa), sub_species
            domesticus) (library: TB3(2-15)) day 14 embryo bone marrow DNA.
  ORGANISM  Gallus gallus
            Eukaryota; Animalia; Chordata; Vertebrata; Aves; Neornithes;
            Neognathae; Galliformes; Phasianidae.
REFERENCE   1  (bases 1 to 435)
  AUTHORS   Benatar,T., Tkalee,L. and Ratcliffe,M.J.
  TITLE     Stochastic rearrangement of immunoglobulin variable-region genes in
            chicken B-cell development
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 89, 7615-7619 (1992)
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     intron          <1..105
                     /gene="IgL"
     CDS             106..>435
                     /pseudo
                     /note="V-J-region"
                     /product="immunoglobulin light chain"
                     /gene="IgL"
                     /codon_start=1
     mat_peptide     123..>431
                     /pseudo
                     /note="V-J-region"
                     /product="immunoglobulin light chain"
                     /gene="IgL"
                     /codon_start=1
     misc_recomb     399..402
                     /note="VJ junction"
                     /gene="IgL"
BASE COUNT       82 a    143 c    119 g     91 t
ORIGIN
        1 gggactgtgg gcacggggct ctgtcccact gctgcgcggg cagggctgtg cgtgcggggc
       61 cgtcactgat tgccgttttc tcccctctct cctctccctc tccaggttcc ctggtgcagg
      121 cagcgctgac tcagccgtcc tcggtgtcag cgaacccggg aggaaccgtc aagatcacct
      181 gctccgggga tagcagctac tatggctggt accagcagaa ggcacctggc agtgcccctg
      241 tcactgtgat ctatgacaac accaacagac cctcgaacat cccttcacga ttctccggtt
      301 ccaaatccgg ctccacagcc acattaacca tcactggggt ccgagccgac gacaatgctg
      361 tctattactg tgcgagtaca gacagcagca gtactgctgc atggtatatt tggggccggg
      421 acaaccctga ccgtc
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human chromosome 4 sequence-tagged site STS4-245
Date: 21 Aug 92 10:08:07 GMT
Distribution: bionet
Checksum: 40551     3

LOCUS       HUM4STS245    456 bp ds-DNA             PRI       21-AUG-1992
DEFINITION  Human chromosome 4 sequence-tagged site STS4-245
ACCESSION   L00759
KEYWORDS    STS; human chromosome 4; sequence tagged site.
SOURCE      Homo sapiens, clone p4-187 from a chromosome 4 plasmid library;
            vector pBluescript II KS + (Stratagene)
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 456)
  AUTHORS   Goold,R.D., diSibio,G., Dugaiczyk,A., Smith,K.A., Xu,H.,
            Magrane,G., Lang,D.B., Cox,D.R., Masters,S.B. and Myers,R.M.
  TITLE     Sequence-Tagged Sites for Human Chromosome 4
  JOURNAL   Unpublished (1992) see COMMENT for author address
  STANDARD  full automatic
COMMENT     PCR components: 25 ng of human genomic DNA, 10 pmol of each
            oligonucleotide, 200 micro-M dNTPs, 0.25 U Taq  polymerase (Cetus)
            in 10 micro-l of 50 mM KCl-20 mM  Tris-HCl, pH 8.3 (at room temp),
            2.5 mM MgCl-2.  Initial denaturation at 94degC for 1.5 min, then 30
            cycles of  94degC for 15 sec, 62degC for 23 sec, and 72degC for 30
            sec, followed by a final extension at 72degC for 3.5 min, using a
            Perkin-Elmer 9600 thermocycler. PCR-amplified product size 205 bp.
            Sequence submitted by:
            Human Genome Mapping Center
            Box 0925
            University of California San Francisco
            San Francisco, CA 94143-0925  USA
            Phone: (415) 502-1612   Fax: (415) 476-8391
            e-mail: hgmcprobes@cgl.ucsf.edu
FEATURES             Location/Qualifiers
     misc_feature    13..35
                     /label=Primer1
     misc_feature    complement(193..217)
                     /label=Primer2
BASE COUNT      117 a     94 c    106 g    134 t      5 others
ORIGIN
        1 gatcacggtg tcactagggt tggcttcttc taaggcctct ccttggcttg cagatggttc
       61 ccttctccct gtgtcttcat gtcgtctttc ctctgtccat gcagcacatg tctgcatcca
      121 aatttnctct tcttataagg acagcaacta tgttgtatga gggctcatat taaagaccta
      181 tttgaactta ttgtctgttc aatagttgta tctccaaata cagttgcatt gtgaggtact
      241 aggaattaca acttcagcat atgaattntt agggtcacaa ttcagcccat aacaatttct
      301 ctggccactc tggggaggat aactgtctga gggagacagn ggcgangagc aaaaacaagg
      361 tcagctgaga ggctatttca acagtctagg tgagagatag taaaagcttg gctccagngg
      421 gtactaaaga gtgggggaaa agtgaccact agttct
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human chromosome 4 sequence-tagged site STS4-105
Date: 21 Aug 92 10:06:34 GMT
Distribution: bionet
Checksum: 36900     3

LOCUS       HUM4STS105    421 bp ds-DNA             PRI       21-AUG-1992
DEFINITION  Human chromosome 4 sequence-tagged site STS4-105
ACCESSION   M95229
KEYWORDS    STS; human chromosome 4; sequence tagged site.
SOURCE      Homo sapiens, clone C4-54 from Los Alamos National Laboratory
            chromosome 4 cosmid library, plate 4-1-178R, pos 10D; vector
            SuperCos 1 (Stratagene)
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 421)
  AUTHORS   Goold,R.D., diSibio,G., Dugaiczyk,A., Smith,K.A., Xu,H.,
            Magrane,G., Lang,D.B., Cox,D.R., Masters,S.B. and Myers,R.M.
  TITLE     Sequence-Tagged Sites for Human Chromosome 4
  JOURNAL   Unpublished (1992) see COMMENT for author address
  STANDARD  full automatic
COMMENT     PCR components: 25 ng of human genomic DNA, 10 pmol of each
            oligonucleotide, 200 micro-M dNTPs, 0.25 U Taq  polymerase (Cetus)
            in 10 micro-l of 50 mM KCl-20 mM  Tris-HCl, pH 8.3 (at room temp),
            2.5 mM MgCl-2.  Initial denaturation at 94degC for 1.5 min, then 30
            cycles of  94degC for 15 sec, 62degC for 23 sec, and 72degC for 30
            sec, followed by a final extension at 72degC for 3.5 min, using a
            Perkin-Elmer 9600 thermocycler. PCR-amplified product size 113 bp.
            Sequence submitted by:
            Human Genome Mapping Center
            Box 0925
            University of California San Francisco
            San Francisco, CA 94143-0925  USA
            Phone: (415) 502-1612   Fax: (415) 476-8391
            e-mail: hgmcprobes@cgl.ucsf.edu
FEATURES             Location/Qualifiers
     misc_feature    62..86
                     /label=Primer1
     misc_feature    complement(149..174)
                     /label=Primer2
BASE COUNT      113 a     92 c     50 g    130 t     36 others
ORIGIN
        1 tctctgtgag aatcaaagtc caagantaaa catttattca ctgtaagtat caattgggta
       61 agcttctaga cattctttct acctacaata acttttttta caaaactaat attttggggg
      121 ttttcttcac ctttatttct agcctngnta ttcaacatat tagttatcct caccntngnt
      181 tcaatgatga cgnctatgtg tntaagccnc ctatgtctta atctangtca ttaataaaaa
      241 aagcctnntn ngtanntaac gagcaacaag atncttaatt ngnaaccccn nngggcnttg
      301 gcancattcg atnaagcact accctncaca acgattgttc acccacgncc ganttncacc
      361 caantgcatn atcacctnat caanttgttc catnttgtcc acatncattc catccagttt
      421 n
//