From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: P.modestum genes uncI, uncB, uncE, uncF, uncH, uncA, uncG, uncD and
Date: 24 Jul 92 10:09:07 GMT
Distribution: bionet
Checksum: 06933    17

LOCUS       PRPUNC2      8010 bp ds-DNA             BCT       24-JUL-1992
DEFINITION  P.modestum genes uncI, uncB, uncE, uncF, uncH, uncA, uncG, uncD and
            uncC for subunits of the sodium dependent F1F0 ATPase
ACCESSION   X58461
KEYWORDS    F1F0-ATPase; uncA gene; uncB gene; uncC gene; uncD gene; uncE gene;
            uncF gene; uncG gene; uncH gene; uncI gene.
SOURCE      Propionigenium modestum DNA.
  ORGANISM  Propionigenium modestum
            Prokaryotae; Gracilicutes; Scotobacteria;
            Anaerobic gram-negative straight, curved and helical rods;
            Bacteroidaceae.
REFERENCE   1  (bases 5888 to 7195)
  AUTHORS   Amann,R., Ludwig,W., Laubinger,W., Dimroth,P. and Schleifer,K.H.
  TITLE     Cloning and sequencing of the gene encoding the beta subunit of the
            sodium ion translocating ATP synthase of Propionigenium modestum
  JOURNAL   FEMS Microbiol. Lett. 56, 253-260 (1988)
  STANDARD  full automatic
REFERENCE   2  (bases 886 to 2954)
  AUTHORS   Esser,U.
  JOURNAL   Unpublished (1990)
  STANDARD  full automatic
REFERENCE   3  (bases 886 to 2954)
  AUTHORS   Esser,U., Krumholz,L.R. and Simoni,R.D.
  TITLE     Nucleotide Sequence of the F0-subunits of the Na-dependent F1F0
            ATPase of Propionigenium modestum
  JOURNAL   Nucleic Acids Res. 18, 5887-5888 (1990)
  STANDARD  full automatic
REFERENCE   4  (bases 1 to 8010)
  AUTHORS   Krumholz,L.R.
  JOURNAL   Unpublished (1991)
  STANDARD  full automatic
REFERENCE   5  (bases 1 to 885; 2955 to 5887; 7196 to 8010)
  AUTHORS   Krumholz,L.R., Esser,U. and Simoni,R.D.
  TITLE     Nucleotide sequence of the F1 subunits of the sodium dependent F1F0
            ATPase of Propionigenium modestum
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
COMMENT     *source: strain=DSM 2376; *source: tissue_library=lambda dash;
            X58461 supercedes X53960
            From EMBL    entry PMUNC2;  dated 15-APR-1992.
FEATURES             Location/Qualifiers
     CDS             709..1092
                     /product="F1 subunit"
                     /gene="uncI"
                     /codon_start=1
                     /translation="MNQEIKKILKNSLITSLIVLVYGIVVRNPIVYFGMFVGCLISTF
                     CFYMICQEAESAMKSGSPFKMTVTGYMKRYAIYGIYLGILVKFFGFPVFLGGAVGLLN
                     IKFNIFLKVVSTQFEKIKKKLSSLK"
     RBS             1112..1115
     CDS             1120..1989
                     /product="F0 subunit"
                     /gene="uncB"
                     /codon_start=1
                     /translation="MKKMGPIILAVVIAIGTFALKMMGVIGFKTPPLVEGPKIMFYVP
                     LPEAMHDFPFAMEMASGVYGFPVTITVISTWFVMLFLIMVFRWSSKNLEVVPERKQAF
                     FETIYGFLDDLIGQLLGNWKKKYFTYIGTLFLFLLISNIVSFFPIPGFSSENGVFSIA
                     PALRTPTADLNTTVGLALLTTYSFIAASFRTSGFFGFFKGLFEPMPLMFPINLAGEFA
                     KPTNISIRLFGNMFAGMVILGLLYKAAPVLIPAPLHLYFDLFSGVVQSFVFIMLTMVY
                     IQGSIGDAEYLED"
     RBS             2036..2040
     CDS             2053..2322
                     /product="F0 subunit"
                     /gene="uncE"
                     /codon_start=1
                     /translation="MDMVLAKTVVLAASAVGAGAAMIAGIGPGVGQGYAAGKAVESVA
                     RQPEAKGDIISTMVLGQAIAESTGIYSLVIALILLYANPFVGLLG"
     RBS             2416..2421
     CDS             2445..2936
                     /product="F0 subunit"
                     /gene="uncF"
                     /codon_start=1
                     /translation="MPAVSIDINMFWQIINFLILMFFFKKYFQKPIAKVLDARKEKIA
                     NDLKQAEIDKEMAAKANGEAQGIVKSAKTEANEMLLRAEKKADERKETILKEANTQRE
                     KMLKSAEVEIEKMKEQARKELQLEVTDLAVKLAEKMINEKVDAKIGANLLDQFIGEVG
                     EEK"
     CDS             3035..3457
                     /product="F1 subunit"
                     /gene="uncH"
                     /codon_start=1
                     /translation="MELYNTDKEFKTLVDHPLIKREDKKEFAKKIFGELEESSLNIIF
                     YLIEKDRLSSIRGIVAEYLKIYYAKNQILDVEAIFAIEPTKDQKAKLIEQLEKKTGKK
                     VNLEVSIDKSIIAGGIIKIGDEIIDGSVRRQLDTIARS"
     CDS             3676..4908
                     /product="F1 subunit"
                     /gene="uncA"
                     /codon_start=1
                     /translation="MALNLEENNVGAVILGDPTGVKEGDEVRATGQIAAVGAGEALLG
                     RVVNSLGEPIDGKGELKTEKMMPLDRKAYGIISRKPVHEPLQTGIKSIDGMVPIGRGQ
                     RELIIGDRQTGKTAVALDAIINQKDTGVKCIYVAIGQKRSTVAQIVKRLEDAGALEYT
                     IVVAATASESAPLQYMAPYTGVSMGEYFMDKGEHVLIVYDDLSKHAVAYREMSLLLKR
                     PPGREAFPGDVFYLHSRLLERAAKLSDEIGAGSITALPIIETQAGDVSAYIPTNVISI
                     TDGQIFLDSQLFNSGFRPAINAGISVSRVGGAAQIKAMKQVAAQVKLELAQYNELLTF
                     AQFGSDLDKATLAQLERGHRIMEILKQEQYKPFVVEEQVVSFFTVINGYLDDIAIDQV
                     RRFEKELLEELKTTQLFS"
     CDS             5008..5856
                     /product="F1 subunit"
                     /gene="uncG"
                     /codon_start=1
                     /translation="MAAGKEIKSRISSVQSTRQITKAMEIVSSTKFKKFQALVNQSKP
                     YSGSMDKVLANLAAGIKNERHPLFDGKTEVKRIGIIVMTSDRGLCGGFNSSTLKEMEK
                     LIVANPDKEVSVIAIGKKGRDYCKKKDRDLKAEYIQLIPETMFDKAKEISENIVEYFY
                     EDIFDEVYLIYNEFISALSTELIVKKLLPIERIEVQDNTTYIFEPSVEDILSSLLPKY
                     LNIQLYQAILENTASEHSARKNAMKNATDNAEDMIKDLTLQYNRERQAAITQEISEIV
                     SGASAL"
     CDS             5906..7195
                     /product="beta subunit"
                     /gene="uncD"
                     /codon_start=1
                     /translation="MENKGVITQIIGPVVDVTFENELPRIYNALKIDRGNGEYLVAEV
                     QQHLGNSVVRAVAMDATDGLQRGMEVVDTGPAITVPVGKAVLGRILNVLGEPVDEAGE
                     VKAEEYAPIHREAPAFEDQGTEKEVFETGIKVVDLLAPYVKGGKIGLFGGAGVGKTVL
                     IMELINNIAQGHGGLSVFAGVGERTREGRDLYDEMLESGVLDKTSLVYGQMNEPPGAR
                     LRVGLTGLTMAENFRDKLATDMGALQERITSTKTGSITSVQAVYVPADDLTDPAPATT
                     FTHLDATTVLSRRIASLGIYPAVDPLDSTSTALEPQIIGHEHYNTAREVQQILQRYKE
                     LQDIIAILGMDELSDEDKVTVNRARKIERFFSQPFHVAEQFTGMDGKYVTVKETIRGF
                     KEIIEGKHDDLPEQAFLYVGTIDEAIAKARELMKGAE"
     CDS             7198..7611
                     /product="F1 subunit"
                     /gene="uncC"
                     /codon_start=1
                     /translation="MATFKLEVVTPLKKVLDRDAEMVIMRTIEGDMGVMADHAPFVAE
                     LAVGEMKIKSANGEEAYFVSGGFLEISKEKTMILADEAIDVKEIDVERAKREAEIAKE
                     TLVKLKEDKDIAVTQKSLQEALTKVRIAEQYMHHL"
BASE COUNT     2809 a   1271 c   1742 g   2188 t
ORIGIN
        1 gatctaaaaa gtttatttga aagagttcac aaccatctca atgatggagg gatatatata
       61 tttgatgttg tagacaggat gtttatggat gagatgttcc caggtggata ttttgtcgat
      121 gaaagagatg atatgaccat aatatgggaa cattatttcg agggtggact ggactacata
      181 aatacgactt tctttgtgaa atattcggat aagttgtatg aaaaacacta tgaagagtat
      241 gtaaaaaaag ttttctcata ttcagaatta aaagatgctg cagacaaatc aaatttgatt
      301 gttgagggta tatttgaaga aaataaggtg gcaggagaga gatgttttta tgtcttgaaa
      361 aaatctgaac taacaattta gtaagcaggt gttgtcaatg gataatgata acaataacaa
      421 taataataat aaatttggca agtacaagga tattatctac tatttatctc tactaagcta
      481 tctagggttt gtagtcatat tcaacattct tttttttgtg gtgatataca agctcattga
      541 aaaatatttt ttctcaagca cagtcctttt tatagcattt gttattttgg gggttgtaag
      601 cggtttctac aatgcgtata aattggttat gaagaaaaaa taaccaaatc aaagtggaat
      661 gcaattgtat aaattgtgtt ctccagaaaa ttttttcgcg gtgataaaat gaatcaggaa
      721 ataaaaaaaa tattaaaaaa ttccttgata acctccttaa tagtactggt ttacggaatt
      781 gtagtcagaa atcccattgt ttattttggg atgtttgtag ggtgcttaat ctcaaccttt
      841 tgtttttata tgatatgtca agaggcagag tcagccatga aaagcgggtc accgtttaaa
      901 atgactgtta caggctatat gaaaagatat gctatttacg gtatatattt aggaatcctg
      961 gttaagttct tcggatttcc ggttttctta ggaggagccg tagggcttct aaacataaag
     1021 ttcaatattt ttctcaaggt ggtttcaacg caatttgaaa aaataaagaa gaaactaagc
     1081 agtttaaaat gattaaagaa agggggtaaa tggagagaaa tgaaaaaaat gggacctatc
     1141 atattagcag tagtaatcgc tattggaacc ttcgcgttga agatgatggg agtcattggt
     1201 tttaaaactc cgcctttagt ggaggggcca aagataatgt tttatgtgcc tctgcctgaa
     1261 gctatgcacg attttccttt tgcaatggaa atggctagtg gggtttacgg attcccggta
     1321 acaataacgg ttataagtac ttggtttgtc atgctttttc tgataatggt atttagatgg
     1381 agttcaaaga atctggaagt ggttcctgaa aggaaacaag ccttttttga aacaatttat
     1441 ggatttcttg atgatctcat cggtcagttg ttaggaaatt ggaagaaaaa atactttact
     1501 tacattggaa cattgttcct attcctactt atttcaaata tagtttcgtt ttttccgatt
     1561 ccaggcttct catcagagaa tggagtgttt tcaatagcac cggccttaag aacaccgaca
     1621 gcagacctta atactacagt tggcctggca ttacttacaa cttacagctt tatagctgcc
     1681 tcgtttagga cttcaggatt ctttgggttt ttcaaaggat tatttgaacc aatgcctctt
     1741 atgtttccga tcaacctagc gggagaattt gcaaaaccaa cgaatatttc aatcagactt
     1801 tttggtaaca tgttcgcagg gatggtaatc ttagggctac tttataaagc agcacctgta
     1861 ttaatcccag caccgcttca cctgtacttc gatcttttca gtggagtggt acaaagtttc
     1921 gtattcatca tgctgacaat ggtttatatt caaggatcta ttggagatgc agagtattta
     1981 gaagattagt tttaaacagt tttaaacaaa atattaataa aaaagaaatt aatttaagga
     2041 gggaatcaag atatggatat ggtattagct aaaactgtag tattagcagc atcagctgtt
     2101 ggtgcaggag cagcaatgat cgcaggtatt ggaccagggg ttggacaagg gtatgcagca
     2161 ggtaaagcgg tagaatctgt tgccagacaa ccagaagcaa aaggggacat catctctaca
     2221 atggtactag gacaagcgat tgcggaatca actggtatct actcactagt tattgcgtta
     2281 atcctacttt acgcaaaccc atttgttgga ttacttgggt aattattaaa gggggtaagc
     2341 taaccattta taaggtagct gccaaatttc gacaagaaaa ctttgcattt attgcataga
     2401 gatcgcttca tgggaaggag gtagacaact tggcaccaca aaatatgcct gctgtgtcta
     2461 ttgacatcaa tatgttttgg cagatcatta actttttgat cttaatgttt ttctttaaaa
     2521 aatattttca aaagccgatc gccaaagtgt tagatgccag aaaagagaaa atagctaatg
     2581 atttaaaaca ggctgaaatc gataaagaga tggcagccaa ggccaacggg gaagctcagg
     2641 gaatcgttaa atcagctaaa actgaggcca acgagatgct tttaagagct gaaaagaaag
     2701 ccgacgaaag aaaagaaact atcctaaaag aagcaaatac tcaaagagag aaaatgctta
     2761 agtctgctga agtagaaatc gagaagatga aagagcaggc aagaaaagag cttcaattag
     2821 aagttactga cttagcagtt aaacttgcag aaaaaatgat caacgaaaag gttgacgcta
     2881 agataggagc aaacctactt gaccaattca ttggagaggt aggggaagag aaatgataga
     2941 agcacaagtt ggtagaagat atgcagaagc tatttatgaa atagctgaat caaatgataa
     3001 tgtgaaagag ctttatgaaa ctttaaatgg agtaatggag ctttataaca ctgacaagga
     3061 gttcaaaacc cttgtagatc atcctctcat aaaaagagaa gataaaaaag agtttgccaa
     3121 gaaaattttt ggggaattag aggagagtag cttaaatatt atcttttatc taatagaaaa
     3181 agatagatta tccagtatca gagggattgt agcagagtac cttaaaatat actacgctaa
     3241 aaatcaaata ctggatgtag aggcgatttt cgcaatagaa cctactaaag atcaaaaagc
     3301 taaacttatc gaacaactcg aaaagaaaac aggtaaaaaa gttaaccttg aggttagcat
     3361 agataaatct ataatcgccg gtggaattat taaaatcgga gacgaaataa ttgacggaag
     3421 tgtcagaaga cagcttgata caatcgctag aagctagtat cttgccattt taaaggtagg
     3481 aggtgtaatc acttgaagat cagaccggaa gagatcagcg gcattattaa gaccgagatc
     3541 gagaattata aaaaatcttt agatgtaaag acttcaggtt ccgttgtaca agttggagat
     3601 ggaatcgcta gaatatacgg tcttagcaat gcaaaagccg gagaattatt agaatttcct
     3661 aacgggatta cagggatggc gcttaacctt gaagaaaaca acgttggtgc cgttatacta
     3721 ggtgatccta caggagttaa agagggagac gaagtaagag ctacaggaca gattgccgct
     3781 gttggagcag gggaagcatt attagggaga gtagtaaact ctcttggaga acctatcgac
     3841 ggtaaaggtg aactgaaaac tgaaaagatg atgccactag atagaaaggc atacggaatc
     3901 atttcaagaa aacctgtaca tgagccactt cagactggta tcaagtcaat cgacgggatg
     3961 gtaccaatcg gaagaggaca aagagaactt atcatcggtg atagacagac tggtaaaaca
     4021 gctgttgcac tagatgcaat cataaaccaa aaagatactg gagtaaaatg tatttacgta
     4081 gcaattggtc aaaaaagatc aactgtagct caaatcgtaa aaagacttga ggatgcagga
     4141 gctcttgaat acacaatcgt agttgcggct acagcttcag aatcagcacc acttcaatac
     4201 atggcaccat atacaggggt atctatgggt gaatacttca tggataaagg ggaacatgta
     4261 ttaatagtat atgatgatct ttctaagcac gcagttgcat acagagagat gtcactacta
     4321 cttaaaagac ctcctggaag ggaagcattc ccaggggatg tattctatct acattcgaga
     4381 ctactagaaa gagctgctaa gctttctgat gagattgggg ctggatcaat tacggcactt
     4441 cctataatcg aaactcaggc aggggatgta tcagcataca taccaacaaa tgttatatct
     4501 ataacagatg ggcaaatctt cctagattca caactattca actcaggatt cagaccagca
     4561 atcaatgccg gtatatctgt atcaagggtt ggaggggcag ctcaaatcaa agctatgaag
     4621 caggttgctg ctcaggttaa actagagctg gctcagtaca atgaactatt aacattcgct
     4681 cagttcggat cagatctgga caaagcaaca ttagctcagc ttgaaagagg acacagaatc
     4741 atggagattc ttaagcagga gcaatataaa ccattcgtag ttgaagaaca ggttgtatct
     4801 ttctttacag taatcaatgg atacctagat gatatagcta tcgatcaagt aagaagattc
     4861 gaaaaagagc ttcttgaaga gttaaagaca acacaactat ttagctgaga tcgtagaaaa
     4921 gaaagctata aaagaggatt tagatgcaaa acttagaaaa gccattgaag atttcaaaaa
     4981 gaaatttagt taaagagagg tgagaccatg gctgcgggaa aagagataaa gagtagaata
     5041 agcagtgttc agtcaactcg tcaaatcaca aaggctatgg agatagtttc ctcaactaag
     5101 ttcaaaaaat ttcaggcgct tgttaaccag tctaaaccat actcaggcag tatggataaa
     5161 gtactagcta acctggctgc aggaataaaa aatgaaagac atcctctttt cgatggtaaa
     5221 acagaagtga aaagaatcgg aatcattgtg atgacttcag acagaggact ttgcggagga
     5281 tttaacagtt ccaccttaaa agagatggaa aagctcattg tggctaatcc agacaaagaa
     5341 gtttcagtta ttgcaatagg taaaaaaggt agagactact gcaaaaagaa agatcgtgat
     5401 cttaaagcag agtacatcca gctgattcct gaaacaatgt ttgataaggc gaaagagata
     5461 agtgaaaata tagtagaata tttctacgaa gatatctttg atgaggttta cctgatctat
     5521 aatgaattca tctcagcttt gagtactgaa ctaattgtta aaaaattatt acctatcgaa
     5581 agaatagagg ttcaagataa tactacgtat atcttcgaac catcggtaga ggatattttg
     5641 tctagcctgt tacctaaata cttaaacata caattatatc aggctatttt ggaaaatacg
     5701 gcaagtgagc attctgccag aaagaatgct atgaaaaatg caactgacaa tgcagaagat
     5761 atgatcaaag accttactct tcaatacaac agagagagac aggctgcgat tactcaagaa
     5821 atttctgaaa ttgttagtgg agcttcagcg ctttaagtaa aaagaggcag aagcttgaaa
     5881 aagttaggga aaaggaggca atttggtgga aaataaggga gttattaccc agataatcgg
     5941 tccagttgta gacgttacat tcgaaaatga attgcctagg atttacaacg cccttaaaat
     6001 tgatcgtgga aatggggaat accttgtagc tgaggttcaa cagcacttag gtaatagcgt
     6061 tgtaagagcc gttgcgatgg acgcaactga cggtctacaa agaggaatgg aagtagtgga
     6121 cacaggtcct gcaattactg taccagtagg taaggcagtt cttggaagaa tattaaacgt
     6181 acttggggaa ccagttgacg aagctggtga agtaaaagca gaggaatatg cacctattca
     6241 tagggaagca cctgcatttg aggaccaagg gacagagaaa gaagtatttg aaactggtat
     6301 caaagtagta gatcttctag ctccatatgt aaaaggtgga aaaatcggtc tattcggtgg
     6361 tgcgggagtt ggtaaaacag ttcttataat ggagcttatc aacaacatcg cccaaggaca
     6421 tggtggacta tctgtatttg caggagttgg agagagaaca agagaaggta gagatcttta
     6481 cgacgaaatg cttgagtcag gagtactaga taaaacatca ctagtgtacg gacaaatgaa
     6541 tgagccgcct ggagcaagac ttagagttgg actaactggt cttactatgg cagaaaactt
     6601 cagagacaaa ctggcaactg acatgggtgc tcttcaagag agaatcacat ctactaagac
     6661 tggttctatc acgtcagtac aagctgtata cgtaccagca gatgatttga ctgacccggc
     6721 tccagcaaca acatttacac atcttgatgc aactacagta ctttcaagaa gaatcgcatc
     6781 actgggaatc tatccagcag ttgacccgct agattcaaca tcaacagctc tagaacctca
     6841 aatcatagga catgagcact acaatacagc aagggaagta caacaaatac ttcaaagata
     6901 taaagaactt caagatatca tcgccatcct aggtatggac gagttatctg atgaagataa
     6961 agttacagtt aacagagcca gaaaaatcga aagattcttc tctcaaccgt tccacgttgc
     7021 tgagcaattc actggaatgg acggtaaata tgtaactgtt aaggaaacta taagaggatt
     7081 taaagagatc atagaaggta agcatgatga tcttcctgag caagctttcc tttatgtagg
     7141 tacaattgat gaagcaatag caaaagcaag ggaactgatg aagggagctg aataatcatg
     7201 gcgactttca aattagaagt ggtgacacct ctaaaaaaag ttcttgatag agatgcagaa
     7261 atggtaatta tgagaaccat tgaaggggat atgggtgtta tggcagacca tgctcctttt
     7321 gtggcggaac ttgccgttgg tgagatgaaa ataaaatctg ccaatggaga agaggcttat
     7381 tttgtttctg gaggtttctt agaaatatct aaagaaaaaa ctatgatact tgctgatgag
     7441 gccattgatg taaaagagat cgatgttgag agagctaaga gagaggcaga aattgccaag
     7501 gaaacactcg ttaagctcaa agaggacaaa gatatcgctg ttactcaaaa atctctacaa
     7561 gaagctctta caaaagtaag aattgcagaa caatatatgc atcatctata aaaaaagact
     7621 gtgctttggc acagtctttt tctgtttatt tgacctctac aaatttaaac tttgcctgag
     7681 gtgaagcctt cttgctatgg gatacctcaa tgtcaaccct ctcctccaac aactcatctc
     7741 tgaaaagatt taggagtgta ttttttcttt gatccatcag ctgatcaatt ccggaatcta
     7801 aatcaagaac ttttatatcc cgcctgaaaa tcagatcttt tacattttga accctcaata
     7861 gctctctttc aacataaaaa tctgtgtaga tagtgatgga aactttgaga tcatcatttt
     7921 ctttcagggt ttttaagatt ttttcaagct tcactgattc tccagtagaa atatctgctg
     7981 agagaaattg aaaataaaga gtgtcttccc
//