From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: D.pseudoobscura Adh gene for alcohol dehydrogenase
Date: 28 Dec 92 21:26:31 GMT
Distribution: bionet
Checksum: 16474 8

LOCUS       DROADHGN14   3465 bp ds-DNA             INV       01-DEC-1992
DEFINITION  D.pseudoobscura Adh gene for alcohol dehydrogenase
ACCESSION   X62194
KEYWORDS    ADH gene; alcohol dehydrogenase.
SOURCE      Drosophila pseudoobscura DNA.
  ORGANISM  Drosophila pseudoobscura
            Eukaryota; Animalia; Metazoa; Arthropoda; Uniramia; Insecta;
            Pterygota; Neoptera; Holometabola; Diptera; Brachycera;
            Cyclorrhapha; Schizophora; Drosophiloidea; Drosophilidae.
REFERENCE   1  (bases 1 to 3465)
  AUTHORS   Schaeffer,S.W. and Miller,E.L.
  TITLE     Estimates of gene flow in Drosophila pseudoobscura determined from
            nucleotide sequence analysis of the alcohol dehydrogenase region
  JOURNAL   Genetics 132, 471-480 (1992)
  STANDARD  full automatic
COMMENT     *source: strain=GB112; *source: isolate=Gundlach Bundschu Winery,
            Sonoma Valley, *source: California; *source: chromosome=IV section
            88;
               From EMBL    entry DPADHGN14;  dated 16-OCT-1992.
FEATURES             Location/Qualifiers
     mRNA            join(40..119,915..1020,1084..1488,1551..1966)
                     /note="adult mRNA (Adh)"
     mRNA            join(882..1020,1084..1488,1551..1966)
                     /note="larval mRNA (Adh)"
     promoter        8..14
                     /note="adult promoter (Adh)"
     prim_transcript 40..1966
                     /note="adult primary transcript (Adh)"
     exon            40..119
                     /note="adult exon 1a"
     intron          120..914
                     /note="adult intron"
     promoter        846..853
                     /note="larval promoter (Adh)"
     prim_transcript 882..1966
                     /note="larval primary transcript (Adh)"
     exon            882..1020
                     /note="larval exon 1"
     exon            915..1020
                     /note="adult exon 1b"
     intron          1021..1083
                     /number=1
     exon            1084..1488
                     /number=2
     intron          1489..1550
                     /number=2
     exon            1551..1966
                     /number=3
     polyA_signal    1943..1948
     polyA_site      1966
     CDS             join(928..1020,1084..1488,1551..1817)
                     /EC_number="1.1.1.1"
                     /gene="Adh"
                     /product="alcoholdehydrogenase"
                     /codon_start=1
                     /translation="MSLTNKNVVFVAGLGGIGLDTSRELVKRNLKNLVILDRIDNPAA
                     IAELKAINPKVTITFYPYDVTVPVAETTKLLKTIFAQVKTIDVLINGAGILDDHQIER
                     TIAVNYTGLVNTTTAILDFWDKRKGGPGGIICNIGSVTGFNAIYQVPVYSGSKAAVVN
                     FTSSLAKLAPITGVTAYTVNPGITKTTLVHKFNSWLDVEPRVAEKLLEHPTQTSQQCA
                     ENFVKAIELNKNGAIWKLDLGTLEPITWTQHWDSGI"
BASE COUNT      974 a    791 c    793 g    907 t
ORIGIN      
        1 cgtactgtat ttaaggcact gcacatgtcg aggaccacga ttattgtcta cagtgcagtc
       61 agtagttgtc aggtgcagca ggcggaccag catttcttta aatctactca atcgatcaag
      121 taagtaactg taatccgagt acagtcggcc catcccactc ggttctgccg taactcaaga
      181 aatttcttga tagcgcagtt ccagttgtag aacgtcgttt aaaaaaacat ctcgttggag
      241 cttgggttga aaaacgttcg agttgggcgt agaaaatttc aaatataaaa tatcaaaaaa
      301 gagaaaatat cttcccaaag cagctgtcca ttttttgtta aagtgataga tcaaaaattc
      361 gtatgacgta tttcccaagt gctctttatg caaataattt cctcaattct aattctacaa
      421 gaagcacttt tggctcttgt ccagaaaaaa caatttgttt acacttttcg gttgataaca
      481 gaggcggacc gacggctaag taacataaca gtcgcctgtt aagtaatgcc ctcactctct
      541 gttagatggc cgcgcgtgtc gactccgata gaccagcaga cacgcagtag ttttcctcaa
      601 gaacctgaac tctaaacata gacataattt acgatacctt gagactcaaa taaaaataca
      661 agtatgagtg gtattctatt cctgattaga ctcgcacaca cacacgtacg agtattaaca
      721 taaatttgcc ggcaaataac caaaacaaaa cgaacgcaaa cgatccgaac acgtaatgcg
      781 agagataaga aacagaaaag ctcgacgtga aagaagcttc tggcggaagc tcttgccgaa
      841 ctccctataa atacgagaca gaaaaccaga ataaatataa taacaaactg ttgaattgtc
      901 ccatcgattc ccagatcaaa agacagaatg tctctcacca acaagaacgt cgttttcgtg
      961 gccggtctgg gcggcattgg cctggacacc agccgggagt tggtcaagcg taatctgaag
     1021 gtaagagaga acgaattcca tggaatatat ggaatcctaa atttaaaagt ttatattttt
     1081 tagaacctgg tcatcctgga tcgcattgac aatccggctg ccattgccga actgaaggca
     1141 atcaatccca aggtgaccat caccttctat ccctacgatg tgactgtgcc cgtcgctgag
     1201 accaccaagc tcctgaagac catctttgcc caggtgaaga caatcgatgt cctgatcaac
     1261 ggtgctggca tcctggacga tcatcagatc gagcgcacca ttgccgttaa ctacacgggc
     1321 ctggtcaaca ccaccacagc cattctggac ttctgggaca agcgcaaggg cggcccaggc
     1381 ggcatcattt gcaacattgg ctccgtcacc ggtttcaatg ccatctacca ggtgcccgtt
     1441 tactccggct ccaaggcggc ggtggttaac tttacctcct ccctggcggt aagaaatcct
     1501 acaactatcc caaaatcccc aaaactaaat atttctaaat attcccatag aaactggctc
     1561 ccattactgg tgtcactgct tacactgtca atcctggcat cactaagacc actctggtcc
     1621 acaaattcaa ctcgtggctg gatgtggagc cccgtgtggc agagaagctg ctcgagcatc
     1681 ccacccagac ctctcagcag tgcgccgaga actttgtgaa ggccattgag ctgaacaaga
     1741 acggtgccat ctggaagttg gatttgggca ccttggagcc catcacatgg acccagcact
     1801 gggactcggg catctaaacc atcccagaga ctctatggga catggcgttt tagcttttag
     1861 tttctttttt tccactcaat tgttacgtat atacatacac atatggcaat aaggctgatt
     1921 tgaaccccgt tttgaatatg ataatacaaa attatatttg agaaatttca acaaaatcga
     1981 taagagctat agctctcttt taattaaaaa aaactagata aaaagagcaa tggtcagtgg
     2041 tggtcagtgg tcgttgtggc ctctatcgat ttccacacaa aaactttact gtttagtaat
     2101 agaaaagaac ttgagaaagg cagccaaaag aatgtacgat ctgacgggta agcatgtctg
     2161 ctatgtggct gactgcggtg gcattgcact ggagaccagc aaggttctca tgaccaagaa
     2221 tatagcggtg agtggtagag aggtggtgta gagagtggag agtggttatg gttcttaaac
     2281 aagtctctag tctctagtct ctagtctctt ggctgctgct ggtagctgtc gaaactaatt
     2341 aagttaatga ctttttcatg ttttgttttt gtccgttcaa agctacaatt aagtttatgt
     2401 tttgagtttg atctactttt gcttaaggga agtactttcg attacggatt gctggcatat
     2461 aaaaatagat accaaggaat gtgcatcata tcatattgat tccatcttga ttctttgccc
     2521 agaaactggc catccttcag agcgtggaaa accccccggc catcgctcag ttacaatcga
     2581 ttaagcacag cacacagatc ttcttctgga ccttcgatgt gaccatggcc cgcgaggaga
     2641 tgaagaagta cttcgatgag gtcatggtcc agatggacta catcgatgtg ctgatcaatg
     2701 gggccaccct gtgcgatgag cggaacattg atgccaccat caatacgaat ctgaccggca
     2761 tgatgaacac cgtggccact gtgctgccct atatggaccg taagatgggc ggttcgggtg
     2821 gactgattgt gaatgtcacc tccgtcatag gactggatcc atcgccggtc ttttgtgcgt
     2881 acagtgcctc aaagtttggt gtgattgggt tcacccgaag tctagcggtg agtgggcgag
     2941 atcttagctt caaaatgctc tctaatgtct tccattcttt cgcaggatcc cctgtattac
     3001 acccaaaatg gtgtggctgt catggccgtc tgctgtggcc ccaccaaagt gtttgtcgat
     3061 cgggaactga ctgccttcct ggaatacggc cagtcctttg ccgatcgcct gcgtcgtgcc
     3121 ccctgccaat cgacggccgt ctgcggccag aatatagtaa atgccatcga gagatcggag
     3181 aacggacaga tctggattgc cgacaagggt ggcctggagt cggtggccct ccactggtat
     3241 tggcatatgg ccgatcagtt tgtgaactac atgcagagca ctgacgatga ggatcaggag
     3301 ttttttttgg gccagcgatg aggcatgcct tgatccacgg aataaaacat caaatgtgtc
     3361 acaaaaagta ggcaacttgt ttgtgggtct ggaaatagat ttgaaatatt ttgaggtcat
     3421 atgaataatg acataaatga gggtgcctga aagtatgcca agttt
//