From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: P. aeruginosa transposon-like structure, TnAP41, which carries the
Date: 12 Jan 93 05:49:33 GMT
Distribution: bionet
Checksum: 05893 9

LOCUS       PSEPAP41     3541 bp ds-DNA             BCT       12-JAN-1993
DEFINITION  P. aeruginosa transposon-like structure, TnAP41, which carries the
            genes for pyocin AP41.
ACCESSION   D12705
KEYWORDS    TnAP41; immunity protein; pyocin AP41.
SOURCE      Pseudomonas aeruginosa (strain PAF, isolate PAF41-2) R' plasmid
            library, DNA, clone pYK211.
  ORGANISM  Pseudomonas aeruginosa
            Prokaryotae; Bacteria; Gracilicutes; Scotobacteria;
            Pseudomonadaceae.
REFERENCE   1  (bases 1 to 3541)
  AUTHORS   Sano,Y. and Kageyama,M.
  TITLE     A novel transposon-like structure which carries the genes for
            pyocin AP41, a Pseudomonas aeruginosa bacteriocin having a DNase
            domain of E2-group colicins
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
COMMENT     Submitted (24-JUL-1992) to DDBJ by: Yumiko Sano Molecular Biology
            Mitsubishi kasei Institute of Life Sciences 11 Minamiooya, Machida
            Tokyo 194 Japan Phone:  0427-24-6253 Fax:    0427-29-1252.
FEATURES             Location/Qualifiers
     repeat_unit     143..147
                     /rpt_type=direct
                     /note="one of the duplicated insertion sites of a
                     transposon-like structure TnAP41"
     repeat_unit     148..185
                     /rpt_type=inverted
                     /note="the left terminal end of a transposon-like
                     structure TnAP41"
     misc_signal     395..441
                     /function="a regulatory sequence for the induced
                     expression"
                     /note="P box"
     RBS             538..543
     RBS             2872..2877
     terminator      3168..3199
     repeat_unit     3340..3376
                     /rpt_type=inverted
                     /note="the right terminal end of a transposon-like
                     structure TnAP41"
     repeat_unit     3377..3381
                     /rpt_type=direct
                     /note="one of the duplicated insertion sites of a
                     transposon-like structure of TnAP41"
     CDS             550..2883
                     /function="killing sensitive strains of P. aeruginosa by
                     causing the breakdown of chromosomal DNA. The C-terminal
                     part of this protein carries a DNA endonuclease"
                     /product="the large component of pyocin AP41"
                     /codon_start=1
                     /translation="MSDVFDLGSMTTVATATGQYSFYTPPPPTPIPYLTYIARPGINK
                     FDLPEGAKIKDLIKRYQYIGSQIPAAIMIRGVQEEIKKSTNTALANVGAIVDGELAYL
                     ASQKKEKLNPAEATPLQMASAEKAAAVELLASKQKELADARTIANAFFGYDPLTVNYV
                     NVMNEIYGRREDKDFSFDNWSKSYSAAQKIRLIEAKISVLNSRSSALDGKVAELTRLQ
                     RLEDAQHAAEAARQTEAERLAQEQRQAEARRQAEEARRQAEAQRQAELQRLAEAEAKR
                     VAEAEKKRQDEINARLQAIVVSESEAKRIEEIYKRLEEQDKISNPTVTTPPAVDAGSR
                     VDDALAHTGTRVTSGGETGATGGSGRDVDTGTGQGGITARPVDVGSVSIPDRRDPKIP
                     DQPRRDLGSLVPTFPDFPTFPSFPGVGVPAAAKPLIPAGGGAASVSRTLKTAVDLLSV
                     ARKTPGAMLGQVAAVVATMAVSSFWPKLNNGERQASFAIPVAELSPPLAVDWQAIAAA
                     KGTVDLPYRLKTLNVDGSIQIIAVPTEPGSAAVPVRALTLDSASGTYKYTTTGPGGGT
                     ILVTPDTPPGQIDPSSSTPAVPRGPLIMPGTLLIPKEPQIESYPELDQREFNDGIYVY
                     PEDSGIPPLYIVYRDPRDEPGVATGNGQPVTGNWLAGASQGDGVPIPSQIADQLRGKE
                     FKSWRDFREQFWMAVSKDPSALENLSPSNRYFVSQGLAPYAVPEEHLGSKEKFEIHHV
                     VPLESGGALYNIDNLVIVTPKRHSEIHKELKLKRKEK"
     CDS             2886..3158
                     /function="immunity protein of pyocin AP41"
                     /product="the small component of pyocin AP41"
                     /codon_start=1
                     /translation="MDIKNNLSDYTESEFLEIIEEFFKNKSGLKGSELEKRMDKLVKH
                     FEEVTSHPRKSGVIFHPKPGFETPEGIVKEVKEWRAANGLPGFKAG"
BASE COUNT      850 a    989 c    974 g    728 t
ORIGIN      34 min.
        1 gtcgacgccg aggaactggc ggcgagcagc agccgcaacg ccgccgagct gttcggctgg
       61 gactgatcaa gcgtcgcgca gaagttaaac atcccagttg cgcccttacc attgcggcag
      121 cgccagggcc tcgcgcaggc gctcgtgggg gacgatagct agcaaggaca aaatcgtacg
      181 ctaagcgtgc agccttgcgc agtgaggccc tagaggtagc aggtgaggcg tcagaaaatg
      241 catggataaa ccctaaacct agacattgcc cgccgctctg acgcccagtt agggcgtggc
      301 ccaatttgac atcgcgagaa cttaggatgc ggcagcgcca agctaactcg gtaacgatga
      361 aagcgaatac acggctactg cgacagcaag aggagatatt gtagtttgtt gtcgtgcatc
      421 gctcgtgcat caccgtttat tgagggtcgc tatcttcaat gttccgcagc aaacctgaag
      481 agtcccattt gcctactaga caggcagtga aagcaggttt tcttttatgg gtttcttaag
      541 ggaaatatca tgagcgacgt ttttgacctt ggaagcatga ccacagtagc aaccgctact
      601 ggtcagtact ctttctacac acctccgcca cctaccccta ttccatacct tacctatatc
      661 gcccgtcccg gcatcaataa atttgatctt cctgaagggg cgaaaatcaa ggatctgatt
      721 aagcgctatc agtatattgg ttcgcaaatc ccggcagcaa tcatgattcg tggtgtgcag
      781 gaagagatca aaaaatccac gaacactgcc ttggccaatg tgggggcaat tgtcgatggc
      841 gaactggcgt atcttgctag ccagaaaaag gaaaaattaa atcctgccga ggcgacaccc
      901 ttgcagatgg cctctgctga aaaggccgcg gcggtggaac tgcttgcgtc caaacagaag
      961 gaactggctg acgcacgaac cattgcaaat gcattctttg gctatgaccc tctcacggtc
     1021 aattatgtta atgtaatgaa tgaaatctac ggccgccgcg aagataaaga tttcagtttc
     1081 gacaactggt cgaagtctta ttcagccgca caaaagatcc gcttgatcga agcgaaaatc
     1141 agcgtcctca atagccgctc ctccgccctg gatggcaagg tcgccgaact gacccggctt
     1201 cagcggctgg aggacgcaca gcacgccgca gaggccgccc ggcagacgga agcggagcgc
     1261 ttagcgcaag agcaacgtca agctgaagcc cgacggcagg ccgaagaggc tcgtcgccag
     1321 gccgaggctc agcgacaagc cgaactgcaa cgcttggccg aagcagaggc caagcgagtc
     1381 gcagaggcgg agaaaaagcg tcaagacgaa atcaatgcgc ggcttcaggc cattgtcgta
     1441 tcggaaagtg aggccaagcg catcgaggaa atctacaagc gtctggaaga acaggacaaa
     1501 atcagcaacc ccaccgtcac tacccctccc gcagtcgatg ccggctctcg ggtcgatgac
     1561 gcactcgccc ataccggaac ccgtgtgacc tctggaggag aaactggagc aacgggtggc
     1621 tccggtcggg atgttgatac gggtactgga caaggaggca tcacggcgcg cccggtcgat
     1681 gtcggcagtg tttcaatccc tgaccggcgt gacccgaaaa ttcccgacca gccaaggagg
     1741 gatcttggct cgttggttcc tacttttccc gacttcccga cgttccctag ctttccgggc
     1801 gtcggtgtac cagcagcggc taaacctctg attccagcag gtggcggtgc tgcgagcgtt
     1861 tccaggactc tcaaaaccgc agtggatttg ctttcggtgg cccgcaagac ccctggtgca
     1921 atgttgggtc aggtagccgc tgtcgtcgcc actatggcgg tcagttcctt ctggccgaaa
     1981 ctgaacaatg gtgaacggca ggcctccttt gccattccgg ttgcggaact gtctccgcca
     2041 ctggcggttg actggcaagc cattgccgcc gccaaaggaa cggtggacct gccttaccgg
     2101 ctgaaaaccc tcaatgtcga tgggtcgatt caaatcatcg ccgtaccgac cgagccgggt
     2161 agtgcggcgg tccccgtcag ggcgctgact ctggactccg cctcggggac ctacaaatac
     2221 acgactaccg gccctggcgg cggcaccatc ctagtcaccc cggatactcc acctggccag
     2281 atcgatccgt cgtcttcgac acccgcagtg ccccgtggcc cgctgatcat gcccggcaca
     2341 ctgctgattc cgaaggaacc gcagatcgag tcctatccag aactcgacca acgggagttc
     2401 aacgacggca tctacgtcta cccggaggac agcggtattc cgccactcta tattgtttat
     2461 cgagatccgc gagatgagcc gggcgttgct accggtaatg gccagccggt cactgggaac
     2521 tggctggccg gcgcctcgca aggtgacgga gtgccgattc cgagccaaat cgccgatcag
     2581 ttacgcggta aggaattcaa gagttggcga gactttcggg agcagttttg gatggccgtt
     2641 agcaaagacc catcagctct cgaaaacctc tcccccagta ataggtactt tgtcagtcag
     2701 ggccttgctc cttatgctgt gccagaggag catctgggta gcaaagaaaa gtttgagatt
     2761 caccatgtag tccctctgga aagcggcggt gctctttaca atatagacaa tcttgttatc
     2821 gtcactccga aacgccacag cgaaattcat aaagaactga aacttaaacg taaggagaaa
     2881 taataatgga tattaaaaat aacctttccg attatactga aagcgagttc ctggagatca
     2941 ttgaagagtt cttcaagaac aaatcaggac taaagggtag tgagctagag aagagaatgg
     3001 ataaactggt caagcacttc gaggaagtta cttctcaccc acgaaagagt ggggtaattt
     3061 tccatccgaa acctggcttt gaaacgcccg aaggcatagt gaaagaagtc aaagagtggc
     3121 gtgccgccaa cggcctccct ggcttcaagg ctggctaatt agccaccaac aagccccgtt
     3181 ccggcggggc ttgttttttg agagactttc cttatttttc tggacgctac tcactctagc
     3241 cctgacaagt ccatttaatt ggcttgcaga gttttcggcg gtttcggctt gcgccccagg
     3301 ctgagtcatt gaagagcata ttctcaggct cacagaaacc ttagcgtagg atttttccga
     3361 attctgcggt ttcccctcgt gcaaccgcgc ggcgctgtcc acgtccgcca gctcgatggc
     3421 caggctcgcc gcccggttca gttcctcgtc catggagcct ccgccagcaa tgcgtcgcaa
     3481 cgctcgcgca cgaacgcgcg caagagggtc accgggcggg tcaactgggc gcggtgggta
     3541 c
//