From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: B.subtilis spoIIIAA and spoIIIAB genes.
Date: 11 Sep 92 10:02:21 GMT
Distribution: bionet
Checksum: 26161     4

LOCUS       BACO3A       1243 bp ds-DNA             BCT       11-SEP-1992
DEFINITION  B.subtilis spoIIIAA and spoIIIAB genes.
ACCESSION   X61962
KEYWORDS    spoIIIAA gene; spoIIIAB gene; sporulation.
SOURCE      Bacillus subtilis DNA.
  ORGANISM  Bacillus subtilis
            Prokaryotae; Firmicutes; Endospore-forming rods and cocci;
            Bacillaceae.
REFERENCE   1  (bases 1 to 1243)
  AUTHORS   Illing,N. and Errington,J.
  TITLE     The spoIIIA operon of Bacillus subtilis defines a new temporal
            class of mother-cell-specific sporulation genes under the control
            of the SigE form of RNA polymerase
  JOURNAL   Mol. Microbiol. 5, 1927-1940 (1991)
  STANDARD  full automatic
REFERENCE   2  (bases 1 to 1243)
  AUTHORS   Errington,J.
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
COMMENT     *source: strain=168; *source: clone=Phi105J112;
            From EMBL    entry SPO3A;  dated 26-AUG-1992.
FEATURES             Location/Qualifiers
     -35_signal      134..139
     -10_signal      155..162
                     /note="SigE-dependent promoter"
                     /citation=[2]
                     /citation=[1]
     RBS             183..190
     CDS             199..1122
                     /function="Sporulation,stage III"
                     /product="SpoIIIAA protein"
                     /gene="spoIIIAA"
                     /codon_start=1
                     /translation="MNEIAEVLPESMKNALSEIPEQQWLEIEEVRIRINRPVELIRRG
                     QPVYLSYAGTAEDAHLILSRLSNYSMYTLEEELKKGYVTIRGGHRVGLAGRVITENGG
                     VKGLRDIASFNIRIARQKLGIAEPLLPYLYQNSWLNTLIIGPPQTGKTTLLRDLARLS
                     STGKKNMLPVKTGIVDERSEIAGCLRGIPQHQFGQRIDVLDACPKAEGLMMMIRSMSP
                     EVMIVDEIGRMEDTDALLEALHAGVSVIVSAHGWSISDLMKRPSLKRLWEERAFDRYL
                     ELSRAKGPGTVSQIYDKDGNVLSRTTGVKTC"
                     /citation=[2]
                     /citation=[1]
     CDS             1116..>1243
                     /function="Sporulation,stage III"
                     /product="SpoIIIAB protein"
                     /gene="spoIIIAB"
                     /codon_start=1
                     /translation="MLKLLGAVFIVVATTWTGFEMAKIYTERPRQIRQLRAALQSL"
                     /citation=[2]
                     /citation=[1]
BASE COUNT      340 a    270 c    340 g    293 t
ORIGIN
        1 ccgttgattt ttatcattac aatgactgtc ggaatctacc aaattgccgg gcagctttcg
       61 tatgcaaagc tgattctgat ttttacgggt gttgtcttga ttttggcggg tgttcataaa
      121 taaagagagg cttgtcataa agtctgcctc acatcataca ttttaaagaa gccaatcggt
      181 agaaaggagg aggctctgtt gaatgaaatc gctgaggttc tccctgaatc catgaaaaac
      241 gccctttctg aaataccgga acaacaatgg ctggaaatcg aagaagtcag aattcggatc
      301 aaccgccctg ttgaattgat tcgaagagga caacctgttt atttgtccta tgcgggcacg
      361 gccgaggatg cccatctgat cctgagccgg ctcagcaact atagtatgta tacacttgaa
      421 gaagagctga agaaagggta tgtcacgatc agaggcggcc acagagtcgg actggcgggc
      481 agagtcatta cggaaaacgg aggtgtgaaa ggcctccgtg acatcgcatc ttttaatata
      541 cgaattgccc ggcaaaagct ggggattgcg gagccgctgc ttccatactt atatcaaaac
      601 agctggctga atacgctgat tatcggaccg ccccaaaccg gcaaaacaac acttctccgg
      661 gaccttgcga ggctctcaag caccggcaaa aaaaacatgc tgcccgtaaa gacagggatc
      721 gtcgatgaac ggtcagagat tgccggatgt cttcgcggta ttccgcagca tcaatttggt
      781 cagagaattg acgtgttaga cgcctgtcca aaagctgaag ggctgatgat gatgattcga
      841 tccatgagcc ctgaggtgat gattgtcgat gaaatcgggc gtatggaaga tacagatgca
      901 cttctggagg cgctgcatgc cggtgtttca gtaatcgtat cggcgcatgg ctggagtata
      961 tccgacctga tgaagcggcc ttcattgaag cgtttgtggg aagaaagagc ctttgaccgc
     1021 tatttggaat tatcaagagc aaaagggccg ggcacagtga gccaaattta tgacaaagac
     1081 ggaaatgtgc tgtctaggac gacgggcgtg aaaacatgct gaagctgctg ggcgctgtat
     1141 tcattgtggt agcgacaaca tggacaggtt ttgagatggc gaagatttat acagaacggc
     1201 cgcggcaaat ccgccagctg cgtgcggcgc ttcaatccct gca
//