HDTenEightyP/NetNews huggingface dataset:
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human immunodeficiency virus type 2 (22POLB3) polymerase fragment.
Date: 18 Aug 92 10:01:18 GMT
Distribution: bionet
Checksum: 24750     2

LOCUS       HIV2POLB3     708 bp ss-RNA             VRL       18-AUG-1992
DEFINITION  Human immunodeficiency virus type 2 (22POLB3) polymerase fragment.
ACCESSION   M87137
KEYWORDS    polymerase.
SOURCE      Human immunodeficiency virus type 2 uncultured blood mononuclear
            cells (5/89 sample) RNA.
  ORGANISM  Human immunodeficiency virus type 2
            Viridae; ss-RNA enveloped viruses; Positive strand RNA virus;
            Retroviridae; Lentivirinae.
REFERENCE   1  (bases 1 to 708)
  AUTHORS   Gao,F., Yue,L., White,A.T., Pappas,P.G., Barchue,J., Hanson,A.P.,
            Greene,B.M., Sharp,P.M., Shaw,G.M. and Hahn,B.H.
  TITLE     Human infection by genetically-diverse SIVsm-related HIV-2 in West
            Africa
  JOURNAL   Nature (1992) In press
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     misc_feature    1..708
                     /note="PCR derived HIV-2 polymerase fragment"
                     /note="putative"
                     /citation=[1]
BASE COUNT      260 a    131 c    168 g    149 t
ORIGIN
        1 gttagggatt atagcagggc agccaacaga aacagagtcc ccactagtaa acagaatcat
       61 agaagaatta ataaggaagg aagctgttta catcagctgg gtgccaactc acaagggtct
      121 cggaggaaat caagaagtag accacctagt aagtcaagga attaggcaag tattattctt
      181 agaaaaaata gaaccagcac aggaagagca tgaaagattt catagcaatg ccaaagagct
      241 gacacacaaa tttggtatac ctaacttagt ggctaaacag atagtaaatg cttgtcatgt
      301 gtgtcaacag aaaggagagg ccatacatgg gcaagtaaat gcagatttag gaacatggca
      361 aatggactgt acccatttag aaggaaaagt tatcatagta gcagtccatg tggccagtgg
      421 ctttatagaa gcagaggtaa tcccacaaga aactggcaga caaacagctc tttttctgtt
      481 aaaattggca gcaagatggc ctattaccca cctacacaca gataatggtg ccaatttcac
      541 ttcacaggaa gtaaaaatgg tggcgtggtg ggtaggcgtg gaacatacct ttggagtacc
      601 ttacaaccca cagagtcagg gagtagtaga ggccatgaac catcatctga aaaaccaaat
      661 agatagaatc agagagcagg caaacacaat ggagaccata gtcttgat
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human chromosome 4 sequence-tagged site STS4-517
Date: 21 Aug 92 10:10:55 GMT
Distribution: bionet
Checksum: 46735     3

LOCUS       HUM4STS517    421 bp ds-DNA             PRI       21-AUG-1992
DEFINITION  Human chromosome 4 sequence-tagged site STS4-517
ACCESSION   L00889
KEYWORDS    STS; human chromosome 4; sequence tagged site.
SOURCE      Homo sapiens, clone p4-599 from a chromosome 4 plasmid library;
            vector pBluescript II KS + (Stratagene)
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 421)
  AUTHORS   Goold,R.D., diSibio,G., Dugaiczyk,A., Smith,K.A., Xu,H.,
            Magrane,G., Lang,D.B., Cox,D.R., Masters,S.B. and Myers,R.M.
  TITLE     Sequence-Tagged Sites for Human Chromosome 4
  JOURNAL   Unpublished (1992) see COMMENT for author address
  STANDARD  full automatic
COMMENT     PCR components: 25 ng of human genomic DNA, 10 pmol of each
            oligonucleotide, 200 micro-M dNTPs, 0.25 U Taq  polymerase (Cetus)
            in 10 micro-l of 50 mM KCl-20 mM  Tris-HCl, pH 8.3 (at room temp),
            2.5 mM MgCl-2.  Initial denaturation at 94degC for 1.5 min, then 30
            cycles of  94degC for 15 sec, 62degC for 23 sec, and 72degC for 30
            sec, followed by a final extension at 72degC for 3.5 min, using a
            Perkin-Elmer 9600 thermocycler. PCR-amplified product size 245 bp.
            STS sequence contains a simple sequence tandem repeat (TTTG).
            Sequence submitted by:
            Human Genome Mapping Center
            Box 0925
            University of California San Francisco
            San Francisco, CA 94143-0925  USA
            Phone: (415) 502-1612   Fax: (415) 476-8391
            e-mail: hgmcprobes@cgl.ucsf.edu
FEATURES             Location/Qualifiers
     misc_feature    155..177
                     /label=Primer1
     misc_feature    complement(375..399)
                     /label=Primer2
BASE COUNT       89 a     78 c     68 g    182 t      4 others
ORIGIN
        1 gatcaagtta gctgttgctg ttgtctgttg tgtttttcat ttcgttcatc atattcttca
       61 gtttcagaat tcttctttta aaatattctt tccatctctt tgttaaactt ctagtttnng
      121 ttcatgtatt gttttcctga cgtcattgag ttttctgtgt tctcctatac ctcactgagc
      181 ttccttaaaa actattattt tgaaatcttt gtcagacaat gtatgtatct tcatttcttt
      241 gggggtggtt aatggaaaat tattgtgctt ctttggaaga aatgtgtttt cttaaaantg
      301 tttgtttgtt tgtttctggc acccttgcct ttctgtcttt gcatttgaaa aagcagtccc
      361 ccccagtttt taatgtctca ttttagcaga taaacacctg nactatcacc cagcgaggac
      421 c
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human chromosome 4 sequence-tagged site STS4-301
Date: 21 Aug 92 10:08:45 GMT
Distribution: bionet
Checksum: 11173     3

LOCUS       HUM4STS301    725 bp ds-DNA             PRI       21-AUG-1992
DEFINITION  Human chromosome 4 sequence-tagged site STS4-301
ACCESSION   L00787
KEYWORDS    STS; human chromosome 4; sequence tagged site.
SOURCE      Homo sapiens, clone p4-217 from a chromosome 4 plasmid library;
            vector pBluescript II KS + (Stratagene)
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 725)
  AUTHORS   Goold,R.D., diSibio,G., Dugaiczyk,A., Smith,K.A., Xu,H.,
            Magrane,G., Lang,D.B., Cox,D.R., Masters,S.B. and Myers,R.M.
  TITLE     Sequence-Tagged Sites for Human Chromosome 4
  JOURNAL   Unpublished (1992) see COMMENT for author address
  STANDARD  full automatic
COMMENT     PCR components: 25 ng of human genomic DNA, 10 pmol of each
            oligonucleotide, 200 micro-M dNTPs, 0.25 U Taq  polymerase (Cetus)
            in 10 micro-l of 50 mM KCl-20 mM  Tris-HCl, pH 8.3 (at room temp),
            2.5 mM MgCl-2.  Initial denaturation at 94degC for 1.5 min, then 30
            cycles of  94degC for 15 sec, 62degC for 23 sec, and 72degC for 30
            sec, followed by a final extension at 72degC for 3.5 min, using a
            Perkin-Elmer 9600 thermocycler. PCR-amplified product size 364 bp.
            STS sequence contains a simple sequence tandem repeat (CA).
            Sequence submitted by:
            Human Genome Mapping Center
            Box 0925
            University of California San Francisco
            San Francisco, CA 94143-0925  USA
            Phone: (415) 502-1612   Fax: (415) 476-8391
            e-mail: hgmcprobes@cgl.ucsf.edu
FEATURES             Location/Qualifiers
     misc_feature    209..233
                     /label=Primer1
     misc_feature    complement(551..573)
                     /label=Primer2
BASE COUNT      223 a    155 c    109 g    214 t     24 others
ORIGIN
        1 gatcgtgcca ctgcactcca gcctgggtga cagagcaaga ccctgtctca aaaaagaaaa
       61 aaaaaagagt aaactgaggc acgcctgctt caaaagtgtc tgtcatcaac atctttctct
      121 agctctgtgt cccattggta atatttaaat gttaatttng ggtttttcgc catcttagaa
      181 gattaatgag gaaatgttgg cataatattt agcgtttctt ggaagaaagc ttctggtgaa
      241 tacaattatc gntgngcatt ttcataataa tgaaatatca cactatttnn atattcttgt
      301 nctctttaat taatctttcc agtcccatca caatcaactt attttctana gccanngact
      361 tnggngtagg taaccnttct gttttctcct aagtttnnnt ncacacacac acacacacac
      421 acacacacac acacacacac acacacacac acacacacac acacacacat atatanagat
      481 atatatatat atatatatat atataatttc nttgattcca gcctcaagta tttatcactt
      541 ttgcttttgt tttccctgag ggtcagagct gtagggctga aagatattaa accctgcgtc
      601 agagaagagt ggtatctccc ccggtggaca gatgacctta tcagatttga atttaggggn
      661 ncnaancaac caaccgtagg ttaaaacttt aactactncc acagaattaa natctgttcc
      721 tctta
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human chromosome 4 sequence-tagged site STS4-153
Date: 21 Aug 92 10:07:02 GMT
Distribution: bionet
Checksum: 32643     3

LOCUS       HUM4STS153    300 bp ds-DNA             PRI       21-AUG-1992
DEFINITION  Human chromosome 4 sequence-tagged site STS4-153
ACCESSION   M95243
KEYWORDS    STS; human chromosome 4; sequence tagged site.
SOURCE      Homo sapiens, clone C4-101 from Los Alamos National Laboratory
            chromosome 4 cosmid library, plate 4-1-1R, pos 11D; vector SuperCos
            1 (Stratagene)
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 300)
  AUTHORS   Goold,R.D., diSibio,G., Dugaiczyk,A., Smith,K.A., Xu,H.,
            Magrane,G., Lang,D.B., Cox,D.R., Masters,S.B. and Myers,R.M.
  TITLE     Sequence-Tagged Sites for Human Chromosome 4
  JOURNAL   Unpublished (1992) see COMMENT for author address
  STANDARD  full automatic
COMMENT     PCR components: 25 ng of human genomic DNA, 10 pmol of each
            oligonucleotide, 200 micro-M dNTPs, 0.25 U Taq  polymerase (Cetus)
            in 10 micro-l of 50 mM KCl-20 mM  Tris-HCl, pH 8.3 (at room temp),
            2.5 mM MgCl-2.  Initial denaturation at 94degC for 1.5 min, then 30
            cycles of  94degC for 15 sec, 62degC for 23 sec, and 72degC for 30
            sec, followed by a final extension at 72degC for 3.5 min, using a
            Perkin-Elmer 9600 thermocycler. PCR-amplified product size 111 bp.
            Sequence submitted by:
            Human Genome Mapping Center
            Box 0925
            University of California San Francisco
            San Francisco, CA 94143-0925  USA
            Phone: (415) 502-1612   Fax: (415) 476-8391
            e-mail: hgmcprobes@cgl.ucsf.edu
FEATURES             Location/Qualifiers
     misc_feature    51..74
                     /label=Primer1
     misc_feature    complement(147..171)
                     /label=Primer2
BASE COUNT       80 a     40 c     71 g     90 t     19 others
ORIGIN
        1 aatgaacagg catggtatga ttttgaattt ttttcaatgt atgtgaattt gctttatggt
       61 tgagcatgtg gttgatctta gagtatgttg tattagggtt ctgtagaggg acagcactat
      121 taggatacat gtatatttga aagggagttt attaaggagt gttgactcac acaatcatag
      181 ggngangtnt cctantgggn ggttgcncan tnannaccca annnngtctg tctnnntttc
      241 aanacttcaa aagcaggaaa gccaacagtg caaacttcag tctgtggctg anggcccaaa
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: M.musculus pva gene for parvalbumin, exon 2 (5'-part)
Date: 22 Aug 92 10:01:11 GMT
Distribution: bionet
Checksum: 60077     2

LOCUS       MUSPVAEX21     76 bp ds-DNA             ROD       22-AUG-1992
DEFINITION  M.musculus pva gene for parvalbumin, exon 2 (5'-part)
ACCESSION   X62827
KEYWORDS    cytosolic calcium binding protein; parvalbumin; pva gene.
SOURCE      Mus musculus DNA.
  ORGANISM  Mus musculus
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Rodentia; Myomorpha; Muridae; Murinae.
REFERENCE   1  (bases 1 to 76)
  AUTHORS   Schleef,M.
  JOURNAL   Unpublished (1991)
  STANDARD  simple staff_entry
REFERENCE   2  (sites)
  AUTHORS   Schleef,M., Zuehlke,C.H., Jockusch,H. and Schoeffl,F.
  TITLE     The structure of the mouse parvalbumin gene
  JOURNAL   Mamm. Genome 3, 217-225 (1992)
  STANDARD  full staff_review
COMMENT     *source: strain=ssp. domesticus, strain=A2G; *source: stage=adult;
            *source: chromosome=15;
            From EMBL    entry MMPVAEX21;  dated 01-JUL-1992.
FEATURES             Location/Qualifiers
     prim_transcript <1..>76
     intron          1..48
                     /number=1
     misc_feature    11..17
                     /note="splice branch point consensus motif"
                     /product="parvalbumin"
                     /gene="pva"
                     /label=pva_CDS
     exon            49..>76
                     /number=2
                     /note="5'part ofprotein coding region"
BASE COUNT       17 a     20 c     17 g     22 t
ORIGIN
        1 ggagaacact ttttaatctt ttcgcacttg ctctgcccgc tcaaacagtt gcaggatgtc
       61 gatgacagac gtgctc
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human chromosome 4 sequence-tagged site STS4-415
Date: 21 Aug 92 10:10:06 GMT
Distribution: bionet
Checksum: 55315     3

LOCUS       HUM4STS415    603 bp ds-DNA             PRI       21-AUG-1992
DEFINITION  Human chromosome 4 sequence-tagged site STS4-415
ACCESSION   L00847
KEYWORDS    STS; human chromosome 4; sequence tagged site.
SOURCE      Homo sapiens, clone p4-354 from a chromosome 4 plasmid library;
            vector pBluescript II KS + (Stratagene)
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 603)
  AUTHORS   Goold,R.D., diSibio,G., Dugaiczyk,A., Smith,K.A., Xu,H.,
            Magrane,G., Lang,D.B., Cox,D.R., Masters,S.B. and Myers,R.M.
  TITLE     Sequence-Tagged Sites for Human Chromosome 4
  JOURNAL   Unpublished (1992) see COMMENT for author address
  STANDARD  full automatic
COMMENT     PCR components: 25 ng of human genomic DNA, 10 pmol of each
            oligonucleotide, 200 micro-M dNTPs, 0.25 U Taq  polymerase (Cetus)
            in 10 micro-l of 50 mM KCl-20 mM  Tris-HCl, pH 8.3 (at room temp),
            2.5 mM MgCl-2.  Initial denaturation at 94degC for 1.5 min, then 30
            cycles of  94degC for 15 sec, 66degC for 23 sec, and 72degC for 30
            sec, followed by a final extension at 72degC for 3.5 min, using a
            Perkin-Elmer 9600 thermocycler. PCR-amplified product size 177 bp.
            STS sequence contains a simple sequence tandem repeat (CA).
            Sequence submitted by:
            Human Genome Mapping Center
            Box 0925
            University of California San Francisco
            San Francisco, CA 94143-0925  USA
            Phone: (415) 502-1612   Fax: (415) 476-8391
            e-mail: hgmcprobes@cgl.ucsf.edu
FEATURES             Location/Qualifiers
     misc_feature    198..222
                     /label=Primer1
     misc_feature    complement(351..374)
                     /label=Primer2
BASE COUNT      178 a    143 c    141 g    124 t     17 others
ORIGIN
        1 gatctgcagc aggaaatagg gggtgaagtg atgacagtta gcactcccat ctctccctct
       61 gcctctagca cagccttcac gcagcctggc caagtgtatt gacagacaac acatctcagg
      121 gttcatttct aaagaaactg aaccaactgt ggcagcaggg agtaagagat ggcttgtgaa
      181 ggcttctgat gcgaattctg atacctcaga gtaaatactt cctcacggca ttttgggggt
      241 gaagaaaagc tggccagcag acaacccagt nctaancaca cacacacaca cacacacaca
      301 cacacacaca cacacacaca cagngtgctt catttagaat tctcactcag ggaagaggga
      361 taactgagga aaaagaagga aaaaaaaaaa gctctncana cacatctttt tnaaagagca
      421 gaggaaactt tcccatcttt ctttccntat tatgnatagg gggcccantc ccccngggna
      481 accagtaaca tgngggggga actgtccctg taaacgnagt ttttatttgg ggnacccaag
      541 agtgctgtta cttcaccaga gtggccagcc atacgtggta nctgnggctg nggcgggagg
      601 atg
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Leuconostoc mesenteroides recombination enzyme (recA) gene, partial
Date: 19 Aug 92 10:02:34 GMT
Distribution: bionet
Checksum: 00127     2

LOCUS       LEUIL1250     312 bp ds-DNA             BCT       19-AUG-1992
DEFINITION  Leuconostoc mesenteroides recombination enzyme (recA) gene, partial
            cds.
ACCESSION   M94060
KEYWORDS    recA gene; recombination enzyme.
SOURCE      Leuconostoc mesenteroides (library: IL1250) DNA.
  ORGANISM  Leuconostoc mesenteroides
            Prokaryotae; Firmicutes; Firmibacteria; Gram-positive cocci.
REFERENCE   1  (bases 1 to 312)
  AUTHORS   Duwat,P., Ehrlich,S.D. and Gruss,A.
  TITLE     A general method for cloning recA gene of gram-positive bacteria by
            polymerase chain reaction
  JOURNAL   J. Bacteriol. 174, 5171-5175 (1992)
  STANDARD  full staff_entry
FEATURES             Location/Qualifiers
     CDS             <1..>312
                     /function="SOS induction"
                     /product="recombination and repair protein"
                     /gene="recA"
                     /codon_start=1
                     /translation="KYAEALGVQKDELLLSQPDTGEQGLEIADALVQSGAVDIIVVDS
                     VAALVPRAEIEGEMGDSHVGLQARLMSQALRKLAGTLNRTGTIAIFINQIREKIGVMF
                     GN"
BASE COUNT       92 a     52 c     77 g     91 t
ORIGIN
        1 aagtacgctg aggcattggg cgtacaaaaa gatgaattac tgctatcaca accagatact
       61 ggcgaacaag gcttagaaat tgctgatgcg ctagtacaat caggtgcggt cgatattatt
      121 gttgttgatt cagtcgcagc ccttgtacct cgagctgaaa ttgaaggtga aatgggtgat
      181 tcgcatgttg gtttgcaagc acgattaatg agtcaagcgc ttcgtaaatt agctgggact
      241 ttaaatcgca caggaaccat tgctattttt attaaccaaa ttcgtgaaaa aattggtgtg
      301 atgtttggta at
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Odocoileus virginianus texanus cytochrome b gene, 3' end.
Date: 19 Aug 92 10:03:00 GMT
Distribution: bionet
Checksum: 35052     2

LOCUS       ODOLOBCB      307 bp ds-DNA             ORG       19-AUG-1992
DEFINITION  Odocoileus virginianus texanus cytochrome b gene, 3' end.
ACCESSION   M98492
KEYWORDS    cytochrome b.
SOURCE      Mitochondrion Odocoileus virginianus (chromosome Mitochondrion
            Odocoileus virginianus, sub_species texanus) adult liver DNA.
  ORGANISM  Mitochondrion Odocoileus virginianus
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Artiodactyla; Ruminantia; Pecora; Cervidae; Odocoileinae.
REFERENCE   1  (bases 1 to 307)
  AUTHORS   Carr,S.M. and Hughes,G.A.
  TITLE     The direction of hybridization between species of North American
            deer (Odocoileus) as inferred from mitochondrial cytochrome b
            sequences
  JOURNAL   J. Mammal. (1992) In press
  STANDARD  full staff_entry
FEATURES             Location/Qualifiers
     CDS             <1..298
                     /product="cytochrome b"
                     /codon_start=3
                     /translation="FGSLLGICLILQILTGLFLAMHYTSDTMTAFSSVTHICRDVNYG
                     WIIRYMHANGASMFFICLFMHVGRGLYYGSYTFLETWNIGVILLFTVMATAFVG"
BASE COUNT       88 a     76 c     47 g     96 t
ORIGIN
        1 acttcggctc tctgctagga atttgcttaa tcctacaaat tcttactggt ctattcctag
       61 caatacatta tacatccgac acaataacag cattctcctc tgtcactcac atctgccgag
      121 acgttaacta tggctgaatc attcgataca tacatgccaa tggagcatcc atattcttta
      181 tctgtctatt catacacgtg ggacgaggac tatactacgg atcatatact ttcttagaaa
      241 catgaaacat tggagtaatt ctcctattca cagttatagc cacagcattc gtagggtatg
      301 tcctacc
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human immunodeficiency virus type 2 (22ENVBB3) envelope (gp 41)
Date: 18 Aug 92 10:01:13 GMT
Distribution: bionet
Checksum: 44068     2

LOCUS       HIV2ENVBB3    453 bp ss-RNA             VRL       18-AUG-1992
DEFINITION  Human immunodeficiency virus type 2 (22ENVBB3) envelope (gp 41)
            fragment.
ACCESSION   M87126
KEYWORDS    envelope protein; glycoprotein.
SOURCE      Human immunodeficiency virus type 2 uncultured blood mononuclear
            cells (5/89 sample) RNA.
  ORGANISM  Human immunodeficiency virus type 2
            Viridae; ss-RNA enveloped viruses; Positive strand RNA virus;
            Retroviridae; Lentivirinae.
REFERENCE   1  (bases 1 to 453)
  AUTHORS   Gao,F., Yue,L., White,A.T., Pappas,P.G., Barchue,J., Hanson,A.P.,
            Greene,B.M., Sharp,P.M., Shaw,G.M. and Hahn,B.H.
  TITLE     Human infection by genetically-diverse SIVsm-related HIV-2 in West
            Africa
  JOURNAL   Nature (1992) In press
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     misc_feature    1..453
                     /note="PCR derived HIV-2 envelope (gp 41) fragment"
                     /note="putative"
                     /citation=[1]
BASE COUNT      154 a     75 c    112 g    112 t
ORIGIN
        1 gacgtggtca aaagacaaca ggaaatgttg cggctgaccg tctggggaac taaaaacctc
       61 cagactagag tcactgccat cgagaagtac ctaaaggacc aagcaaaact aaattcatgg
      121 ggatgtgcct ttagacaggt ttgccacact actgtgccat gggtgaacga cagtatgcag
      181 ccagagtggc aaaacatgac ttggcaacaa tgggaaaaac agattgcctt cttggaggat
      241 aatatcacag agctgttaga gcaagctcaa atacagcagg agaaaaatat gtatgagttg
      301 cagaaattaa atagctggga tgtttttggc aattggcttg atctcacctc ttgggtaaaa
      361 tatatctatt taggatttta tatagtagca ggagtaatag tgttaagaat agctatctat
      421 gtagtacaaa tgttaatgag acttaggaag ggc
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human branched chain alpha-keto acid dehydrogenase (BCKDHB) E1-beta
Date: 20 Aug 92 10:02:06 GMT
Distribution: bionet
Checksum: 08537     3

LOCUS       HUMBCKD05     186 bp ds-DNA             PRI       20-AUG-1992
DEFINITION  Human branched chain alpha-keto acid dehydrogenase (BCKDHB) E1-beta
            subunit gene, exon 5.
ACCESSION   D90386
KEYWORDS    branched chain alpha-keto acid dehydrogenase;
            maple syrup urine disease.
SEGMENT     5 of 10
SOURCE      Homo sapiens adult peripheral blood DNA.
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 16 to 171)
  AUTHORS   Matsuda,I., Asaka,J., Akaboshi,I., Endo,F., Mitsubuchi,H. and
            Nobukuni,Y.
  TITLE     Maple syrup urine disease: Complete primary structure of the
            E-1-beta subunit of human branched chain alpha-ketoacid
            dehydrogenase complex deduced from the nucleotide sequence and a
            gene analysis of patients with this disease
  JOURNAL   J. Clin. Invest. 86, 242-247 (1990)
  STANDARD  simple staff_review
REFERENCE   2  (bases 1 to 186)
  AUTHORS   Mitsubuchi,H., Nobukuni,Y., Endo,F. and Matsuda,I.
  TITLE     Structural organization and chromosomal localization of the gene
            for the E-1-beta subunit of human branched chain alpha-keto acid
            dehydrogenase
  JOURNAL   J. Biol. Chem. 266, 14686-14691 (1991)
  STANDARD  full staff_review
COMMENT     These data kindly submitted in computer readable form by: Hiroshi
            Mitsubuchi Department of Pediatrics Kumamoto University Medical
            School 1-1-1 Honjo, Kumamoto 860 Japan Phone:  096-344-2111 x5654
            Fax:    096-366-3471
FEATURES             Location/Qualifiers
     intron          <1..15
                     /number=4a
                     /gene="BCKDHB"
     exon            16..171
                     /number=5
                     /note="E-1-beta subunit"
                     /gene="BCKDHB"
     intron          172..>186
                     /number=5a
                     /gene="BCKDHB"
BASE COUNT       35 a     45 c     43 g     63 t
ORIGIN      chromosome 6p22-p21
        1 ctttctgacc ctcagattgt taatgaagct gccaagtatc gctatcgctc tggggatctt
       61 tttaactgtg gaagcctcac tatccggtcc ccttggggct gtgttggtca tggggctctc
      121 tatcattctc agagtcctga agcatttttt gcccattgcc caggaatcaa ggtatgttca
      181 tttatg
//