From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Clostridium thermohydrosulfuricum amylopullulanase (apu) gene,
Date: 30 Jul 92 10:01:03 GMT
Distribution: bionet
Checksum: 48860    10

LOCUS       CLOALU       5001 bp ds-DNA             BCT       30-JUL-1992
DEFINITION  Clostridium thermohydrosulfuricum amylopullulanase (apu) gene,
            complete cds.
ACCESSION   M97665
KEYWORDS    amylopullulanase.
SOURCE      Clostridium thermohydrosulfuricum (strain 39E) DNA.
  ORGANISM  Clostridium thermohydrosulfuricum
            Prokaryotae; Firmicutes; Endospore-forming rods and cocci;
            Bacillaceae.
REFERENCE   1  (bases 1 to 5001)
  AUTHORS   Mathupala,S.P., Saha,B.C. and Zeikus,J.G.
  TITLE     Substrate competition and specificity at the active site of
            amylopullulanase from Clostridium thermohydrosulfuricum
  JOURNAL   Biochem. Biophys. Res. Commun. 166, 126-132 (1990)
  STANDARD  full staff_entry
FEATURES             Location/Qualifiers
     RBS             319..326
                     /gene="apu"
     CDS             332..4777
                     /function="alpha amylase and pullulanase enzyme"
                     /product="amylopullulanase"
                     /gene="apu"
                     /evidence=EXPERIMENTAL
                     /codon_start=1
                     /translation="MFKRRTLGFLLSFLLIYTAVFGSMPVQFAKAETDTAPAIANVVG
                     DFQSKIGDSDWNINSDKTVMTYKGNGFYEFTTPVALPAGDYEYKVALNHSWEGGGVPS
                     QGNLSLHLDSDSVVTFYYNYNTSSVTDSTKYTPIPEEKLPRIVGTIQSAIGAGDDWKP
                     ETSTAIMRDYKFNNVYEYTANVPKRYYEFKVTLGPSWDINYGLNGEQNGPNIPLNVAY
                     DTKITFYYDSVSHNIWTDYNPPLTGPDNNIYYDDLKHDTHDPFFRFAFGAIKTGDTVT
                     LRIQAKNHDLESAKISYWDDIKKTRTEVPMYKIGQSPDGQYEYWEVKLSFDYPTRIWY
                     YFILKDGTKTAYYGDNDEQLGGVGKATDTVNKDFELTVYDKNLDTPDWMKGAVMYQIF
                     PDRFYNGDPLNDRLKEYSRGFDPVEYHDDWYDLPDNPNDKDKPGYTGDGIWNNDFFGG
                     DLQGINDKLDYLKNLGISVIYLNPIFQSPSNHRYDTTDYTKIDELLGDLDTFKTLMKE
                     AHARGIKVILDGVFNHTSDDSIYFDRYGKYLDNELGAYQAWKQGDQSKSPYGDWYEIK
                     PDGTYEGWWGFDSLPVIRQINGSEYNVKSWADFIINNPNAISKYWLNPDGDKDAGADG
                     WRLDVANEIAHDFWVHFRAAINTVKPNAPMIAELWGDASLDLLGDSFNSVMNYLFRNA
                     VIDFILDKQFDDGNVVHNPIDAAKLDQRLMSIYERYPLPVFYSTMNLLGSHDTMRILT
                     VFGYNSANENQNSQEAKDLAVKRLKLAAILQMGYPGMPSIYYGDEAGQSGGKDPDNRR
                     TFSWGREDKDLQDFFKKVVNIRNENQVLKTGDLETLYANGDVYAFGRRIINGKDVFGN
                     SYPDSVAIVVINKGEAKSVQIDTTKFVRDGVAFTDALSGKTYTVRDGQIVVEVVALDG
                     AILISDPGQNLTAPQPITDLKAVSGNGQVDLSWSAVDRAVSYNIYRSTVKGGLYEKIA
                     SNVTQITYIDTDVTNGLKYVYSVTAVDSDGNESALSNEVEAYPAFSIGWAGNMNQVDT
                     HVIGVNNPVEVYAEIWAEGLTDKPGQGENMIAQLGYRYIGDGGQDATRNKVEGVEINK
                     DWTWVDARYVGDSGNNDKYMAKFVPDMVGTWEYIMRFSSNQGQDWTYTKGPDGKTDEA
                     KQFIVVPSNDVEPPTALGLQQPGIESSRVTLNWSLSTDNVAIYGYEIYKSLSETGPFV
                     KIATVADTVYNYVDTDVVNGKVYYYKVVAVDTSFNRTASNIVKATPDIIPIKVIFNVT
                     VPDYTPDDGANIAGNFHDAFWNPSAHQMTKTGPNTYSITLTLNEGTQLEYKYARGSWD
                     KVEKGEYGEEIANRKITVVNQGSNTMVVNDTVQRWRDLPIYIYSPKDNTTVDANTNEI
                     EIKGNTYKGAKVTINDESFVQQENGVFTKVVPLEYGVNTTKIHVEPSGDKNNELTKDI
                     TITVIREEPVQEKEPTPTPESEPAPMPEPQPTPTPEPQPSAIMAL"
                     /citation=[1]
     sig_peptide     332..424
                     /gene="apu"
                     /codon_start=1
     mat_peptide     425..4774
                     /function="alpha amylase and pullulanase enzyme"
                     /product="amylopullulanase"
                     /gene="apu"
                     /codon_start=1
                     /citation=[1]
BASE COUNT     1767 a    771 c   1059 g   1404 t
ORIGIN
        1 ctgttcttac tgtttacaat acttttagca gaaaaagcag ataagatgga gataacacaa
       61 atgttaaaac aaatatgaat ttttcaaaga gggatgctta tgtgcatcct tttctttttt
      121 gcaaggtatt gacaaaatat atggggttgg ctataataac aataaggcag cgaaaacgat
      181 tgcgcaaaaa tttgcacaag ggatgtgtat ttataaacac atttatcttt ttgcaattta
      241 tgaaagcgat tgcctaaaag aaatgggggt ggtgttgtaa aggagctttt cgtaagattt
      301 ttaaataaaa acataggtaa agggggatgt agtgtttaag aggagaacat taggcttttt
      361 attgtcattt cttttaattt atacagcagt gtttggctca atgcctgtgc aatttgcaaa
      421 agctgagaca gatacggcgc ctgctatagc caatgttgtt ggcgattttc aatcaaagat
      481 tggagattct gactggaata taaacagtga caaaacagta atgacatata aaggtaatgg
      541 cttttatgaa tttactaccc cagttgcgtt acctgcaggt gattatgagt ataaagttgc
      601 tcttaatcat tcatgggaag gtggaggagt tccttcacaa ggtaatttaa gcttgcatct
      661 tgattcagat tctgtagtaa ctttttatta caactataat acttcaagtg ttactgattc
      721 tacaaaatat acaccaattc cggaagaaaa acttccaaga attgtaggta ctatacaatc
      781 agcaatagga gcaggtgatg attggaaacc tgaaacatcg acagctataa tgagagacta
      841 taagtttaac aatgtttacg aatacactgc aaatgttcca aaaaggtatt atgagtttaa
      901 agtaacttta gggccctcat gggatataaa ttatggctta aatggtgaac aaaatggtcc
      961 aaatattcct ttgaatgtag cctatgatac taagattaca ttttactatg attcggtttc
     1021 acataatata tggacagatt acaatccacc tctcacaggg cctgataata acatatatta
     1081 tgacgattta aaacatgaca cccatgaccc attcttccgc ttcgctttcg gtgcaataaa
     1141 aacaggtgat acagtgactt tgaggataca ggctaaaaat catgaccttg agtcagctaa
     1201 aatttcttat tgggatgata ttaaaaaaac aagaacagaa gtcccgatgt ataaaattgg
     1261 tcaaagtcct gacgggcaat atgaatactg ggaagtgaag ttaagctttg actatcccac
     1321 aagaatttgg tattacttta tacttaaaga cgggacaaaa actgcttatt acggagataa
     1381 cgatgaacaa ttaggtggag taggtaaagc cacagatacg gtaaataaag actttgaact
     1441 tactgtatac gataaaaatt tagacacccc tgattggatg aaaggggcag taatgtatca
     1501 aatattccca gatagatttt acaatggtga ccctttaaat gaccgcctaa aggaatacag
     1561 tagaggtttt gatcctgttg aatatcatga cgactggtat gaccttcccg acaatccgaa
     1621 tgataaagat aaacctggat atacagggga tggtatatgg aataatgact tctttggtgg
     1681 tgatttacaa ggtataaatg ataaattgga ttatctaaaa aaccttggaa tatcagttat
     1741 ttatctcaat ccaattttcc aatcaccttc caatcaccga tatgatacaa ccgattacac
     1801 aaagatagac gagttattgg gagatttaga tacatttaaa acacttatga aagaagccca
     1861 tgcaagagga attaaagtaa tacttgatgg cgtcttcaat catacaagtg atgatagtat
     1921 ttattttgat agatacggga agtacttgga taatgaatta ggtgcttatc aagcctggaa
     1981 acagggagat cagtcaaaat ctccatacgg tgactggtac gaaattaagc ctgacggtac
     2041 ctatgagggc tggtggggat ttgacagctt accggtaata aggcagataa acggaagtga
     2101 gtacaatgta aaaagttggg cagattttat cataaataat cctaatgcaa tatctaagta
     2161 ttggttaaat cctgatgggg ataaagatgc aggtgcagat ggctggagat tggatgttgc
     2221 aaatgaaatt gctcacgatt tctgggttca ttttagagct gcaattaata ctgtgaaacc
     2281 aaatgcgcca atgattgcag aactttgggg agatgcttca ttagatttac ttggagattc
     2341 ttttaactct gttatgaact atctttttag aaatgcagtt attgatttta tactcgataa
     2401 acagtttgat gatggaaatg tggttcacaa tcctatagat gcagcaaaac ttgaccaaag
     2461 gcttatgagc atatatgaga gatatcctct tccagtattt tattctacta tgaacctttt
     2521 aggttctcat gacaccatga gaatattgac agtatttgga tataactctg ctaatgaaaa
     2581 tcaaaattct caagaggcga aagaccttgc agttaagagg cttaaacttg ccgcaatatt
     2641 gcaaatgggc tatccgggaa tgccttctat ttactatggt gacgaggcag gacaatctgg
     2701 tggaaaagac ccagataaca ggagaacatt ctcttgggga agagaagata aagatctgca
     2761 ggatttcttt aagaaagtcg taaacataag gaatgaaaat caagttttaa aaacaggaga
     2821 ccttgaaaca ctttatgcaa atggcgatgt ttatgccttt ggaagaagaa ttataaatgg
     2881 aaaagatgta tttggtaatt cttatcctga cagtgtagct attgttgtga ttaataaagg
     2941 tgaggcaaag tcagtacaaa tagatactac taaatttgta agagatggag ttgcttttac
     3001 agatgcctta agtggtaaga catacacggt tcgtgatgga caaattgttg tagaagttgt
     3061 ggcattggat ggggctatac tcatttcaga tccaggacag aatttgacgg cacctcagcc
     3121 aataacagac cttaaagcag tttcaggaaa tggtcaagta gacctttcgt ggagtgcagt
     3181 agatagagca gtaagttata acatttaccg ctctacagtc aaaggagggc tatatgaaaa
     3241 aatagcttca aatgttacgc aaattactta tattgataca gatgttacca atggtctaaa
     3301 gtatgtgtat tctgtaacgg ctgtagatag tgatggaaat gaaagtgctt taagcaatga
     3361 agttgaggca tatccagcat tttctattgg ttgggcagga aatatgaacc aagttgatac
     3421 ccatgtaata ggcgtaaata atccagttga agtttatgct gaaatttggg cagaaggatt
     3481 aacagataaa cctggccaag gggaaaatat gattgcccag ttaggatata ggtatattgg
     3541 agatggtggg caagatgcta cacgcaacaa agtagaaggt gttgaaataa ataaggactg
     3601 gacatgggtt gatgcacggt atgtagggga ttctggaaat aacgacaaat acatggctaa
     3661 atttgtacct gatatggtag gcacatggga atatattatg agattttcct ctaaccaagg
     3721 acaggattgg acgtatacaa aagggccaga tgggaaaaca gatgaagcaa aacagtttat
     3781 tgtcgtgcca tcaaatgatg tagaaccacc tacagctcta ggcttacaac aaccaggaat
     3841 tgaatcctca agagttacac ttaactggag tctctcaact gataatgtag ctatctatgg
     3901 ctacgaaata tacaaatctt taagtgaaac aggaccattt gtaaagattg caactgtggc
     3961 tgacactgtg tataactacg tagatacaga tgtagtaaat ggaaaagtgt actattataa
     4021 agttgtagca gttgatactt cttttaacag aacagcatca aatatagtga aagctacacc
     4081 tgatataata cctatcaaag tgatatttaa tgtaacagtc cctgattata ctcctgatga
     4141 cggtgcaaat attgctggaa acttccatga cgctttctgg aatccaagtg cccatcagat
     4201 gacaaagaca ggacctaaca cttacagtat tacattgact ttaaatgaag gaacacagct
     4261 tgaatataaa tatgcaaggg gcagctggga taaggtagaa aaaggtgaat atggagagga
     4321 aattgcaaat agaaaaataa ctgttgtcaa tcaaggttca aataccatgg tggtaaatga
     4381 cacagtgcaa agatggagag acttaccaat atacatttat tctccaaaag ataatactac
     4441 agtagatgca aatacaaacg agatagagat taaaggcaat acctataaag gtgcaaaagt
     4501 aactataaat gatgaatctt ttgtacaaca agaaaatggc gtatttacaa aagtagtgcc
     4561 tcttgaatac ggtgtaaata ctactaaaat acatgtggag ccgagtggtg acaagaataa
     4621 tgaactcaca aaagatataa caataactgt tataagagag gagcctgtcc aggaaaaaga
     4681 accaactcct acgccagagt ctgagccagc accaatgcct gaaccacaac cgacaccaac
     4741 accagaacca cagccttctg caattatggc attgtgactg cctcaacact taatttaaga
     4801 gaaggagcaa gtatcacaag taaaattata ggtactatct gctgggaaag ttgtaaaatg
     4861 gcttgaggaa gtgaatggat ggtacaaagt tgactataac ggcaaagtag gatatgtttc
     4921 aaccaaatat gtttcttcag tgccagatcc atcaaaggta accgttgcaa aatcagtgaa
     4981 agttatagtg aagagcggat t
//