From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: D.pseudoobscura Adh gene for alcohol dehydrogenase
Date: 28 Dec 92 21:26:39 GMT
Distribution: bionet
Checksum: 43047 8

LOCUS       DROADHGN19   3475 bp ds-DNA             INV       01-DEC-1992
DEFINITION  D.pseudoobscura Adh gene for alcohol dehydrogenase
ACCESSION   X62199
KEYWORDS    ADH gene; alcohol dehydrogenase.
SOURCE      Drosophila pseudoobscura DNA.
  ORGANISM  Drosophila pseudoobscura
            Eukaryota; Animalia; Metazoa; Arthropoda; Uniramia; Insecta;
            Pterygota; Neoptera; Holometabola; Diptera; Brachycera;
            Cyclorrhapha; Schizophora; Drosophiloidea; Drosophilidae.
REFERENCE   1  (bases 1 to 3475)
  AUTHORS   Schaeffer,S.W. and Miller,E.L.
  TITLE     Estimates of gene flow in Drosophila pseudoobscura determined from
            nucleotide sequence analysis of the alcohol dehydrogenase region
  JOURNAL   Genetics 132, 471-480 (1992)
  STANDARD  full automatic
COMMENT     *source: strain=GB12; *source: isolate=Gundlach Bundschu Winery,
            Sonoma Valley, *source: California; *source: chromosome=IV section
            88;
               From EMBL    entry DPADHGN19;  dated 16-OCT-1992.
FEATURES             Location/Qualifiers
     mRNA            join(40..119,915..1020,1084..1488,1549..1963)
                     /note="adult mRNA (Adh)"
     mRNA            join(882..1020,1084..1488,1549..1963)
                     /note="larval mRNA (Adh)"
     promoter        8..14
                     /note="adult promoter (Adh)"
     prim_transcript 40..1963
                     /note="adult primary transcript (Adh)"
     exon            40..119
                     /note="adult exon 1a"
     intron          120..914
                     /note="adult intron"
     promoter        846..853
                     /note="larval promoter (Adh)"
     prim_transcript 882..1963
                     /note="larval primary transcript (Adh)"
     exon            882..1020
                     /note="larval exon 1"
     exon            915..1020
                     /note="adult exon 1b"
     intron          1021..1083
                     /number=1
     exon            1084..1488
                     /number=2
     intron          1489..1548
                     /number=2
     exon            1549..1963
                     /number=3
     polyA_signal    1940..1945
     polyA_site      1963
     CDS             join(928..1020,1084..1488,1549..1815)
                     /EC_number="1.1.1.1"
                     /gene="Adh"
                     /product="alcoholdehydrogenase"
                     /codon_start=1
                     /translation="MSLTNKNVVFVAGLGGIGLDTSRELVKRNLKNLVILDRIDNPAA
                     IAELKAINPKVTITFYPYDVTVPVAETTKLLKTIFAQVKTIDVLINGAGILDDHQIER
                     TIAVNYTGLVNTTTAILDFWDKRKGGPGGIICNIGSVTGFNAIYQVPVYSGSKAAVVN
                     FTSSLAKLAPITGVTAYTVNPGITKTTLVHKFNSWLDVEPRVAEKLLEHPTQTSQQCA
                     ENFVKAIELNKNGAIWKLDLGTLEPITWTQHWDSGI"
BASE COUNT      974 a    795 c    792 g    914 t
ORIGIN      
        1 cgtagtgtat ttaaggcact gcacatgtcg aggaccacga ttattgtcta cagtgcagtc
       61 agtagttgtc aggtgcagca ggcggaccag catttcttta aatctactca atcgatcaag
      121 taagtaactg taatccaagt acagtcggcc catcccactc ggttctgccg taactcaaga
      181 aatttcttga tagcgcagtt ccagttgtag aacgtcgctt aaaaaaacat ctcattggag
      241 cttgggttga aaaacgttcg agttgggcgt ataaaagttc aaatataaaa tatcaaaaaa
      301 gagaaaatat cttcccaaag cagctgtcca ttttttgtta aagtgataga tcaaaaattc
      361 gtatgacgta cattcctagt gcacttcaca caaacaattt cctcaattct aattctacaa
      421 gaagcacttt tggctcttgt ccagaaaaaa caatttgttt acacttttcg gttgataaca
      481 gaggcggacc gacggctaag taacataaca gtcgcctgtt aagtaatgcc ctcactctct
      541 gttagatggc cgcgcgtgtc gactccgata gaccagcaga cacgcagtag ttttcctcaa
      601 gaacctgaac tctaaacata gacataattt acgatacctt gagactcaaa taaaaataca
      661 agtatgagtg gtattctatt cctgattaga ctcgcacaca cacacgtacg agtattaaca
      721 taaatttgcc ggcaaataac caaaacaaaa cgaacgcaaa cgatccgaac acgtaatgcg
      781 agagataaga aacagaaaag ctcgacgtga aagaagcttc tggcggaagc tcttgccgaa
      841 ctccctataa atacgagaca gaaaaccaga ataaatataa taacaaactg ttgaattgtc
      901 ccatcgattc ccagatcaaa agacagaatg tctctcacca acaagaacgt cgttttcgtg
      961 gccggtctgg gcggcattgg cctggacacc agccgggagt tggtcaagcg taatctgaag
     1021 gtaagagaga acgaattcca tggaatctat ggaatcctaa atttaaaaat tcatttattt
     1081 tagaacctgg tcatcctgga tcgcattgac aatccggctg ccattgccga actgaaggca
     1141 atcaatccca aggtgaccat caccttctat ccctacgatg tgactgtgcc cgtcgctgag
     1201 accaccaagc tcctgaagac catctttgcc caggtgaaga caatcgatgt cctgatcaac
     1261 ggtgctggca tcctggacga tcatcagatc gagcgcacca ttgccgttaa ctacacgggc
     1321 ctggtcaaca ccaccacagc cattctggac ttctgggaca agcgcaaggg cggcccaggc
     1381 ggcatcattt gcaacattgg ctccgtcacc ggtttcaatg ccatctacca ggtgcccgtt
     1441 tactccggct ccaaggcggc ggtggttaac tttacctcct ccctggcggt aagtaatcca
     1501 acgtttttcc tactccccaa aactaaatat ttctttgaaa ttccatagaa actggctccc
     1561 attactggtg tcactgctta cactgtcaat cctggcatca ctaagaccac tctggtccac
     1621 aaattcaact cgtggctgga tgtggagccc cgtgtggcgg agaagctgct cgagcatccc
     1681 acccagacct ctcagcagtg cgccgagaac tttgtgaagg ccattgagct gaacaagaac
     1741 ggtgccatct ggaagttgga tttgggcacc ttggagccca tcacatggac ccagcactgg
     1801 gactcgggca tctaaaccat cccagagact ctatgggaca tggcgtttta gcttttagtt
     1861 tcttttttcc actcaattgt tacgtatata catacacata tggcaataag gctgatttga
     1921 accccgtttt gaatatgata atacaaaatt atatttgaga aatttcaaca aaatcgataa
     1981 gagctatagc tctcttttaa ttaaaaaaaa ctagataaaa agagcaatgg tcgtcagtgg
     2041 tggtcagtgg tcgttgtggc ctctatcgat ttccacacaa aaactttact gtttagtaat
     2101 agaaaagaac ttgagaaagg cagccaaaag aatgtacgat ctgacgggta agcatgtctg
     2161 ctatgtggct gactgcggtg gcattgcact ggagaccagc aaggttctca tgaccaagaa
     2221 tatagcggtg agtggtagag tggtgtagag agtggagagt ggttctggtt cttaaacaag
     2281 tctctagtct ctagtctcta gtctcttggc tgctgctggt agctgtcgaa actaattaag
     2341 ttaatgactt tttcatgttt tgtttttgtc cgctgacttt tgctgttgaa agctacaatt
     2401 aagtttatgt tttgagtttg atctactttt gcttaaggga agtactttcg attacggatt
     2461 gctggcatat aaaaatagat accaaggaat gtgcatcata tcatattgat tccatcttga
     2521 ttctttgccc agaaactggc catccttcag agcgtggaaa accccccggc catcgctcag
     2581 ttacaatcga ttaagcacag cacacagatc ttcttctgga ccttcgatgt gaccatggcc
     2641 cgcgaggaga tgaagaagta cttcgatgag gtcatggtcc agatggacta catcgatgta
     2701 ctgatcaatg gggccaccct gtgcgatgag cggaacattg atgccaccat caatacgaat
     2761 ctgaccggca tgatgaacac cgtggccact gtgctgccct atatggaccg taagatgggc
     2821 ggttcaggtg gactgattgt gaatgtcacc tccgtcatag gactggatcc atcgccggtc
     2881 ttttgtgcgt acagtgcctc aaagtttggt gtgattgggt tcacccgaag tctagcggtg
     2941 agtgggcgtg atcttaactt gaaaatgctc tctaatatct tccattcttc cgcaggatcc
     3001 cctgtattac acccaaaatg gtgtggctgt catggccgtc tgctgtggtc ccactaaagt
     3061 gtttgtcgat cgcgaactga ctgccttcct ggaatacggc caaacctttg ccgatcgcct
     3121 gcgccgtgcc ccctgccaat cgacggccgt ctgcggccag aatatagtaa atgccatcga
     3181 gagatcggag aacggacaga tctggattgc cgacaagggc ggcctggagt cggtggccct
     3241 ccactggtat tggcatatgg ccgatcagtt tgtgaactat atgcagagta ctgacgatga
     3301 ggatcaggag ttttttttgg ggcagcgatg aggcatgcct tgatccacgg aataaaacat
     3361 caaatgtgtc acaaaaagta ggcaacttgt ttgtgggttt ggaaatagat ttgaaatatt
     3421 ttgaggtcat atgaataatg acataaatga gggtgcctga aagtatgcca agttt
//