From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Guinea pig preproglucagon mRNA, complete cds.
Date: 29 Dec 92 01:27:42 GMT
Distribution: bionet
Checksum: 26204 5

LOCUS       GPIGG        1053 bp ss-mRNA            ROD       08-DEC-1992
DEFINITION  Guinea pig preproglucagon mRNA, complete cds.
ACCESSION   D00014 N00014 X04071
KEYWORDS    glucagon; preproglucagon.
SOURCE      Guinea pig pancreas, cDNA to mRNA, clone gpGCG-2.
  ORGANISM  Cavia porcellus
            Eukaryota; Animalia; Chorqata; Vertebrata; Mammalia; Theria;
            Eutheria; Rodentia; Hystricomorpha; Caviidae; Caviinae.
REFERENCE   1  (bases 1 to 1053)
  AUTHORS   Seino,S., Welsh,M., Bell,G.I., Chan,S.J. and Steiner,D.F.
  TITLE     Mutations in the guinea pig preproglucagon gene are restricted to a
            specific portion of the prohormone sequence
  JOURNAL   FEBS Lett. 203, 25-30 (1986)
  STANDARD  full automatic
COMMENT     A cDNA clone encoding guinea pig preproglucagon has been isolated
            from a pancreatic cDNA library. The predicted amino acid sequence
            of proglucagon is highly conserved in all regions,in comparison to
            other mammals,except for the C-terminal portion of the 29-residue
            glucagon region,in which 5 amino acid substitutions have occurred.
            These changes may serve to offset the reduced receptor-binding
            potency of the highly mutated insulin in this New World species.
               Proglucagon is processed into various small peptides: GRPP,
            Glucagon, GLP 1 and 2 and two spacer peptides
               Data kindly reviewed (20-OCT-1986) by S. Seino

              EMBL features not translated to GenBank features:
              key        from     to       description

              CDS         296    319       spacer peptide 1 (aa 64-71)
              CDS         437    481       spacer peptide 2 (aa 111-125)
              POLYA      1053   1053       polyA site.
FEATURES             Location/Qualifiers
     mRNA            <1..1053
                     /note="gluc mRNA"
     sig_peptide     47..106
                     /codon_start=1
                     /note="glucagon signal peptide"
     mat_peptide     107..202
                     /codon_start=1
                     /note="glicentin-related pancreatic peptide (GRPP)"
     mat_peptide     203..295
                     /codon_start=1
                     /note="glucagon"
     mat_peptide     320..436
                     /codon_start=1
                     /note="glucagon-like peptide 1 (GLP-1)"
     mat_peptide     482..586
                     /codon_start=1
                     /note="glucagon-like peptide 2 (GLP-2)"
     CDS             47..589
                     /note="preproglucagon"
                     /codon_start=1
                     /translation="MKSVYFVAGLFIMLAQGSWQRSLQDTEEKPRSVSASQTDMLDDP
                     DQMNEDKRHSQGTFTSDYSKYLDSRRAQQFLKWLLNVKRNRNNIAKRHDEFERHAEGT
                     FTSDVSSYLEGQAAKEFIAWLVKGRGRRDFPEEVAIVEELGRRHADGSFSDEMNTILD
                     NLATRDFINWLIQTKITDRK"
BASE COUNT      333 a    214 c    209 g    297 t
ORIGIN      Unreported.
        1 ggtgcacccg ttgctagcca cagcctacga gcagaaggta gcaaaaatga agagcgttta
       61 ctttgtggct ggattgttca taatgctagc acaaggcagc tggcaacgtt cccttcaaga
      121 cacagaagag aaacccagat ctgtctcagc ctcccaaaca gacatgcttg atgatccaga
      181 tcagatgaac gaagacaagc gccattcaca gggcacattc accagcgact acagcaagta
      241 cttggattcc aggcgtgctc aacaattttt gaaatggctg ttgaatgtca agaggaacag
      301 gaacaacatt gccaaacgtc atgatgaatt tgagagacat gctgaaggga cctttactag
      361 tgatgtaagt tcttacttgg aaggccaagc tgccaaggaa ttcattgctt ggctggtgaa
      421 aggccgaggg aggcgagact tcccagaaga agtcgccatt gtggaagaac tcggccgcag
      481 acatgccgat ggctcattct cagatgagat gaacactatt cttgacaatc ttgccaccag
      541 agactttatc aactggctta ttcagaccaa aatcactgac aggaagtaag tatgtcactc
      601 ttcaagacca tcttcacatc acctgccgtc cacttggaat gtttgaaatt ttacagttct
      661 gtaattttac agagttgtac tctcgagtat ttctttgcag gctattaaac attttttagc
      721 attgtatagc caaatgatta taaatggaat aaactatcgc cagaatgttg ctaaaatatc
      781 aactttacag tataaaagtc ctgtctcttg tttttatctt attttggttg aagtacccca
      841 acttgtttaa atttagcagt gaaattattt ttctattata tactttgtag atgtaaatta
      901 atccaatctg aaaatatctg catgcaatat caggaaaatg aaaaccttgt agccacagca
      961 gtgaaactga aaagagaact tcttaaagcc tttttcataa aaatgctcag ctttcaatgt
     1021 ctcaaagatt gaattaataa attttcaagc ttc
//