From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: L.mexinana gap gene for glycosomal glyceraldehyde-3-phosphate
Date: 3 Jan 93 22:07:08 GMT
Distribution: bionet
Checksum: 65479 8

LOCUS       LEIGAP       4303 bp ds-DNA             INV       21-DEC-1992
DEFINITION  L.mexinana gap gene for glycosomal glyceraldehyde-3-phosphate
            dehydrogenase
ACCESSION   X65226
KEYWORDS    gap gene; glyceraldehyde-3-phosphate dehydrogenase;
            glycosomal protein; tandemly linked gene.
SOURCE      Leishmania mexicana DNA.
  ORGANISM  Leishmania mexicana
            Eukaryota; Animalia; Protozoa; Sarcomastigophora; Mastigophora;
            Kinetoplastida; Trypanosomatina; Trypanosomatidae.
REFERENCE   1  (bases 1 to 4303)
  AUTHORS   Hannaert,V., Blaauw,M., Kohl,L., Allert,S., Opperdoes,F.R. and
            Michels,P.A.
  TITLE     Molecular analysis of the cytosolic and glycosomal
            glyceraldeyde-3-phosphate dehydrogenase in Leishamania mexicana
  JOURNAL   Mol. Biochem. Parasitol. 55, 115-126 (1992)
  STANDARD  full automatic
COMMENT     *source: clone_library=lambda GEM-11, Sau3A fragments;
               From EMBL    entry LMGAP;  dated 26-NOV-1992.
FEATURES             Location/Qualifiers
     CDS             266..1351
                     /EC_number="1.2.1.12"
                     /gene="gap"
                     /note="glycosomal enzyme"
                     /product="glyceraldehyde 3-phosphatedehydrogenase"
                     /codon_start=1
                     /translation="MAPIKVGINGFGRIGRMVFQAICDQGLIGTEIDVVAVVDMSTNA
                     EYFAYQMKHDTVHGRPKYTVEAVKSSPSVETADVLVVNGHRIKCVKAQRNPADLPWGK
                     LGVDYVIESTGLFTDKLKAEGHIKGGAKKVVISAPASGGAKTIVMGVNQHEYSPASHH
                     VVSNASCTTNCLAPIVHVLTKENFGIETGLMTTIHSYTATQKTVDGVSLKDWRGGRAA
                     AVNIIPSTTGAAKAVGMVIPSTKGKLTGMSFRVPTPDVSVVDLTFRATRDTSIQEIDK
                     AIKKAAQTYMKGILGFTDEELVSADFINDNRSSVYDSKATLQNNLPGEKRFFKVVSWY
                     DNEWAYSHRVVDLVRYMAAKDAASSKM"
     CDS             2101..3186
                     /EC_number="1.2.1.12"
                     /gene="gap"
                     /note="glycosomal enzyme"
                     /product="glyceraldehyde 3-phosphatedehydrogenase"
                     /codon_start=1
                     /translation="MAPIKVGINGFGRIGRMVFQAICDQGLIGTEIDVVAVVDMSTNA
                     EYFAYQMKHDTVHGRPKYTVEAVKSSPSVETADVLVVNGHRIKCVKAQRNPADLPWGK
                     LGVDYVIESTGLFTDKLKAEGHIKGGAKKVVISAPASGGAKTIVMGVNQHEYSPASHH
                     VVSNASCTTNCLAPIVHVLTKENFGIETGLMTTIHSYTATQKTVDGVSLKDWRGGRAA
                     AVNIIPSTTGAAKAVGMVIPSTKGKLTGMSFRVPTPDVSVVDLTFRATRDTSIQEIDK
                     AIKKAAQTYMKGILGFTDEELVSADFINDNRSSVYDSKATLQNNLPGEKRFFKVVSWY
                     DNEWAYSHRVVDLVRYMAAKDAASSKM"
BASE COUNT      865 a   1294 c   1203 g    941 t
ORIGIN      
        1 gtcaacagga gtacttgcac ccgagcagtt catgaacgcc actcccgcac gtgtgacctg
       61 cccccctccc ccgcttcctg cactgtccgg ggcacacaca cacccgctca caagcccctc
      121 ctgtcacgtt tctactacta ctctgtctcc gccctttctc tccccgtcgc tacaccgcac
      181 gcacctccat cacgcgtaag ttttctctct caacacaaca aaaccaacat ccttcacccc
      241 ctacccccac ccccaccact ttgcaatggc tcccatcaag gtcggcatca acggcttcgg
      301 ccgcatcggt cgcatggtat tccaggccat ctgtgaccag ggtcttatcg gcactgagat
      361 cgacgtcgtc gcggtggtgg acatgagcac gaatgccgaa tactttgcat accagatgaa
      421 gcacgacacg gtgcacggcc gcccgaaata cacagtggag gctgtgaaga gcagcccatc
      481 cgtggagacg gcggatgtgc ttgtggtgaa cggccaccgt atcaagtgcg tgaaggcgca
      541 gcgcaaccct gcggacctgc cgtggggcaa gcttggtgtg gactatgtga tcgagtctac
      601 cggcctgttc acggacaagt tgaaggctga gggccacatc aagggtggcg cgaagaaggt
      661 tgtgatcagc gccccggcgt ctggcggcgc caagacgatt gtgatgggcg tgaaccagca
      721 cgagtactcg ccggcgtcgc accacgtggt gtcgaatgcg tcgtgcacga ccaactgcct
      781 ggcccccatt gtgcacgtgc tgacgaagga gaacttcggc atcgagaccg gtctgatgac
      841 caccattcac tcctacacgg caacgcagaa gacggtggac ggcgtgtcgc tcaaggactg
      901 gcgtggtggc cgcgcggcgg ccgtaaacat catcccgagc acgactggcg ccgcgaaggc
      961 cgtgggcatg gtgattccgt ccaccaaggg caagctgact ggcatgtcct tccgcgtgcc
     1021 gacgccggac gtgtccgtcg tggatttgac gttccgcgca acgcgcgaca cgtctatcca
     1081 ggagatcgac aaggccatca agaaggcggc gcagacgtac atgaagggca tcctcggctt
     1141 caccgatgag gagctcgtca gcgccgactt tatcaacgac aaccgcagct ccgtctacga
     1201 ctccaaggcg accctgcaaa acaacctgcc tggagagaag cgcttcttca aggtcgtgtc
     1261 ttggtacgac aacgagtggg cctactcgca ccgcgtggtg gacctggtgc gctacatggc
     1321 cgccaaggac gctgcgagct ccaagatgtg aggtgcgcat atatcgcttt atccccgcca
     1381 ccgacagcgg cagtgcagga cttggtgctg tctcaagcac gcgtaccaca agcggtgtac
     1441 tcgcctgcgt ccagcgggcg attatgccat gcaagtgtcc cccgtgagta gacacaggca
     1501 ctcccaaaaa gtacgacaca cttttcccct tttcccatat ttgtttcttt cgttaagtgg
     1561 atcctccaga agctccctga gccgcatgca catgcaggcg cgtgcttgcg aaggcctcgg
     1621 tgcatttgat tttctattcc ctcacgcacc cggcgcagtg ccacggggca cacaggcgct
     1681 gccttgggtg cggagcggcg agttatgata ggccatagac ggtgtcactg gcaaggttga
     1741 ccgcgtgccg catctcatga gtgtatcaaa catgcaaaca agctcacggg catatggcca
     1801 caggagtctg tcttcatgca cagcgcggcc aggaatgttt gaaagggtcg gtcgtggaag
     1861 ggtgcgcact atacccttca cgcacatgtg acctgccccc ctcccccgct tcctgcactg
     1921 tccgggacac acacacaccc gctcacatgc ccctcctgtc acatttctac tactcctctg
     1981 tctccgccct ttctctcccc gtcgctacac cgcacgcacc tccatcacgc gtaagttttc
     2041 tctctcaaca caacaaaacc aacatccttc accccctacc cccaccccca ccactttgca
     2101 atggctccca tcaaggtcgg catcaacggc ttcggccgca tcggtcgcat ggtattccag
     2161 gccatctgtg accagggtct tatcggcact gagatcgacg tcgtcgcggt ggtggacatg
     2221 agcacgaatg ccgaatactt tgcataccag atgaagcacg acacggtgca cggccgcccg
     2281 aaatacacag tggaggctgt gaagagcagc ccatccgtgg agacggcgga tgtgcttgtg
     2341 gtgaacggcc accgtatcaa gtgcgtgaag gcgcagcgca accctgcgga cctgccgtgg
     2401 ggcaagcttg gtgtggacta tgtgatcgag tctaccggcc tgttcacgga caagttgaag
     2461 gctgagggcc acatcaaggg tggcgcgaag aaggttgtga tcagcgcccc ggcgtctggc
     2521 ggcgccaaga cgattgtgat gggcgtgaac cagcacgagt actcgccggc gtcgcaccac
     2581 gtggtgtcga atgcgtcgtg cacgaccaac tgcctggccc ccattgtgca cgtgctgacg
     2641 aaggagaact tcggcatcga gaccggtctg atgaccacca ttcactccta cacggcaacg
     2701 cagaagacgg tggacggcgt gtcgctcaag gactggcgtg gtggccgcgc ggcggccgta
     2761 aacatcatcc cgagcacgac tggcgccgcg aaggccgtgg gcatggtgat tccgtccacc
     2821 aagggcaagc tgactggcat gtccttccgc gtgccgacgc cggacgtgtc cgtcgtggat
     2881 ttgacgttcc gcgcaacgcg cgacacgtct atccaggaga tcgacaaggc catcaagaag
     2941 gcggcgcaga cgtacatgaa gggcatcctc ggcttcaccg atgaggagct cgtcagcgcc
     3001 gactttatca acgacaaccg cagctccgtc tacgactcca aggcgaccct gcaaaacaac
     3061 ctgcctggag agaagcgctt cttcaaggtc gtgtcttggt acgacaacga gtgggcctac
     3121 tcgcaccgcg tggtggacct ggtgcgctac atggccgcca aggacgctgc gagctccaag
     3181 atgtaggggg gctatgccca tgcacgatag gagcacacgt tgctggtatt cactgagttt
     3241 gccgtgagtt gtgttcgctg ccaacatacg cggcagtttt tttttttcag cgagtttttt
     3301 cgattggatt cgatcgatgc ttttggcgtt cgtgcacgat ctctggaaga agcgtggaag
     3361 ccgttcacga gctctcggcc tgttcgatat gtagaagcgt ggccttgaaa ttcgcatttg
     3421 ttgtcaagtg gtgggaaaaa gactttccct tttagcatgc atccaagcgg cgtggctcgc
     3481 gattgcgcag cttggcatat gtgcgtttgt gccggatttt ttgttgttgt cgtttctcga
     3541 tggatgagct gtctgccgag tacgtacaga tgcatctggt acgcgtggag cacccccacc
     3601 tccctgcctc cttgcacgag aagacggaga gtgcagtatg tcaaacgggg tgcgattggc
     3661 aattgtcggt ggctggatct cttttgagct accctccttt tttcctttgt ttttttttgt
     3721 gatttacgcc gttctctctc tctctctctg ccaccgccct ctttctgtct cgccacacca
     3781 ttggcttttc ttgttttggg ggctctctat tgcgggttgg ggaaagggtc tgacgggtgt
     3841 tggggtgtat cttatacgca cagagacacg catgcatacc cccttcgctg ggcatgtacc
     3901 aatctatgtc tgtatctgct cgcacatacc cacgcatcgt cattccttcc ccctcccgcc
     3961 cttccgtatt tgcttttcct ttctcccgct ttgtttcttg tattaggata cggttgtttt
     4021 ccgtgcaagc caagacatgc aggtgtgcgt atgtgtggat atgtcaatgc tggggaggtg
     4081 gcgcgaagcg gaccggagag taacctgttt cgcatattcc ctccttttct gtttttctcc
     4141 cgctcgatct gtccattttt cgagggcgcc cttgcaatga tgtccccagc gggttggaac
     4201 agcacgtcag tgataagagt atatactata aagagaaacg gatgtcgacg cacacacaca
     4261 cacacacacg aggcaggatg agatggggag ggaggagagc gag
//