From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Lactococcus lactis cysteine aminopeptidase (pepC) gene, complete
Date: 8 Jan 93 05:48:36 GMT
Distribution: bionet
Checksum: 40402 6

LOCUS       LACAPEPC     2520 bp ds-DNA             BCT       07-JAN-1993
DEFINITION  Lactococcus lactis cysteine aminopeptidase (pepC) gene, complete
            cds.
ACCESSION   M86245
KEYWORDS    aminopeptidase; cysteine aminopeptidase; proteolytic enzyme.
SOURCE      Lactococcus lactis (strain AM2, sub_species cremoris) DNA.
  ORGANISM  Lactococcus lactis
            Prokaryotae; Firmicutes; Firmibacteria; Gram-positive cocci;
            Streptococcaceae.
REFERENCE   1  (bases 1 to 2520)
  AUTHORS   Chapot-Chartier,M.-P., Nardi,M., Chopin,M.-C., Chopin,A. and
            Gripon,J.-C.
  TITLE     Cloning and sequencing of pepC, a cysteine aminopeptidase gene from
            Lactococcus lactis subsp. cremoris AM2
  JOURNAL   Appl. Environ. Microbiol. (1993) In press
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     CDS             <1..587
                     /codon_start=1
                     /partial
                     /note="ORF 2"
     promoter        591..619
                     /gene="pepC"
     terminator      2043..2063
                     /gene="pepC"
     CDS             718..2028
                     /gene="pepC"
                     /product="cysteine aminopeptidase"
                     /codon_start=1
                     /translation="MTVTSDFTQKLYENFAENTKLRAVENAVTKNGLLSSLEVRGSHA
                     ANLPEFSLDLTKDPVTNQKQSGRCWMFAALNTFRHKFINEFKTEDFEFSQAYTFFWDK
                     YEKSNWFMEQIIGDVAMDDRRLKFLLQTPQQDGGQWDMMVAIFDKYGIVPKAVYPESQ
                     ASSSSRELNQYLNKLLRQDAEILRYTIEQDGDVQAVKEELLQEVFNFLAVTLGLPPQN
                     FEFAFRNKDNEYKKFVGTPKEFYNEYVGIDLNNYVSVINAPTADKPYNKSYTVEFLGN
                     VVGGKEVKHLNVEMDRFKKLAIAQMQAGETVWFGCDVGQESNRSAGLLTMDSYDFKSS
                     LDIEFTQSKAGRLDYGESLMTHAMVLAGVDLDADGNSTKWKVENSWGKDAGQKGYFVA
                     SDEWMDEYTYQIVVRKDLLSEEELAAYEAKPQVLLPWDPMGALA"
BASE COUNT      887 a    372 c    494 g    767 t
ORIGIN      
        1 cagctgaaca actggtaatt gattattcag agaaaagtgt gatagttcaa aaagaaaaat
       61 taattgcaaa aaatactgac gaaaatttga agctaatttt tactgatgga gtgcaacaaa
      121 aagagattaa ccagtttatc agtaagagtc aagcttttgt tgctggactt atgcatgata
      181 ttggtggggt ttatccaaat catcagcgag tagaaaaagc agaattattt ggtatagaat
      241 tactgacaga agagcgtgaa tttcctttga ttattcatca aaaactttct aaatatctgg
      301 cgcgtgagca ttttaaaatt actgacgaaa atatattgtc agcgattgag tgccacacaa
      361 cactgaagga aaattttaca gagttagatt tgattgtttt tcttgctgac aaaatttctt
      421 gggatggtgg ggataatgca ccattcaaag aaggattact gacagctctg tcagtaaatt
      481 tgcaaagtgc agcactttat tatatagact tcatcatcaa tgatggcttg aaagttgcgc
      541 acccgtggct tttagaagca aagaaagatt tagaaaatca gctttcctag ctgatttttt
      601 tgataattta gattataata gaagaatgta aaaataatag aatatcatta atcaaaatcg
      661 ataatgatta tttttatcga aacttttgtc agaagtttcg atttattggg aggtattatg
      721 acagtaacat cagatttcac acaaaaactc tacgaaaatt ttgctgaaaa cacaaaattg
      781 cgtgcggtgg aaaatgccgt gactaaaaat ggtttgcttt catcactcga agtgcgtggt
      841 tcacatgcag cgaatttgcc tgagttttca cttgacttga caaaagaccc tgtaacgaat
      901 caaaaacaat ctggacgttg ctggatgttt gctgctttga acactttccg tcataaattt
      961 attaatgaat ttaaaacaga agattttgaa ttttcacaag cttatacttt cttctgggat
     1021 aaatatgaaa aatcaaactg gtttatggaa caaattatag gagatgttgc gatggacgac
     1081 cgtcgtttga aattcctctt gcaaactcca caacaagatg gtggtcaatg ggatatgatg
     1141 gtagccattt ttgacaaata tggaattgtt ccaaaagctg tttatcctga atcacaagct
     1201 tcaagtagct cacgcgaatt gaatcaatac ttgaacaaat tgctccgaca agatgctgaa
     1261 atcttacgtt atacaattga gcaagacggt gatgttcaag cggtcaaaga agaactttta
     1321 caagaagttt ttaatttcct tgctgtaact ctaggtttgc caccacaaaa ttttgaattt
     1381 gctttccgca acaaagataa tgaatacaaa aaatttgttg gaacgccaaa agaattctac
     1441 aacgaatatg ttgggattga tttgaacaat tatgtttcgg tcattaatgc tccaactgct
     1501 gataaacctt ataacaaaag ctataccgtt gaatttcttg gtaatgtagt cggtggtaaa
     1561 gaagtcaaac acttgaacgt tgagatggac cgtttcaaaa aattggctat tgcccaaatg
     1621 caagcgggcg aaactgtctg gtttggttgt gacgttggtc aagaatcaaa ccgttcagca
     1681 ggacttttga caatggattc ttatgatttt aaatcttcat tggatattga attcacacaa
     1741 agtaaggctg gacgtcttga ctatggcgaa tcactgatga ctcatgccat ggttttagcg
     1801 ggtgttgatt tagatgctga cggaaattca actaaatgga aagttgaaaa ttcttggggt
     1861 aaagatgccg gtcaaaaagg atatttcgtt gcttctgatg aatggatgga tgaatatact
     1921 tatcaaattg ttgtccgcaa agacctttta agcgaagaag aattagctgc ttatgaagca
     1981 aaacctcaag ttctgcttcc atgggatcca atgggagcct tggcttaatt tgtaaattga
     2041 aacatttaac ttttgttaaa tgttttttta ttgacttaaa gttcacttca agtgttaaaa
     2101 taaaagaatg aatataaaaa aggccgccga cttatcaggg ataaaaacag ataatatcag
     2161 atattatgaa agaattgggc taattcctaa aattgcacgg acagagtctg gaatacgaaa
     2221 tttttcagaa gcgaatatta gaacattaaa attcgtcaaa catatgagag atgcaggagt
     2281 gcaagtagaa cccttgacac gttatatggt attggtaact gaaggaaatc ctaatactaa
     2341 agaataaaga attgaaattc tgaagagtca agttgaacaa ttgagaacag aaattattga
     2401 aaaacaatct gctttagatt atttaacatt caaaattgaa aattatgatg aagtcatgct
     2461 tccaagcgaa atgaatttga atcaaagttc aacagaaaca ataaaaattt gaagaggata
//