From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human profilaggrin (FLG) gene exons 2-3, 5'end.
Date: 29 Dec 92 01:00:52 GMT
Distribution: bionet
Checksum: 44448 9

LOCUS       HUMPROFL02   4166 bp ds-DNA             PRI       04-DEC-1992
DEFINITION  Human profilaggrin (FLG) gene exons 2-3, 5'end.
ACCESSION   L01089 M90967
KEYWORDS    filaggrin; profilaggrin.
SEGMENT     2 of 2
SOURCE      Homo sapiens (library: Stratagene) male caucasian adult placenta
            DNA.
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 4166)
  AUTHORS   Dale,B.A., Resing,K.A. and Lonsdale-Eccles,J.D.
  TITLE     Filaggrin: A keratin filament associated protein
  JOURNAL   J. Biol. Chem. 455, 330-342 (1985)
  STANDARD  full automatic
REFERENCE   2  (bases 1 to 4166)
  AUTHORS   Dale,B.A., Resing,K.A. and Lonsdale-Eccles,J.D.
  TITLE     Filaggrin: A keratin filament associated protein
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
REFERENCE   3  (bases 1 to 4166)
  AUTHORS   Presland,R.B., Haydock,P.V., Fleckman,P., Nirunsuksiri,W. and
            Dale,B.A.
  TITLE     Characterization of the human epidermal profilaggrin gene: genomic
            organization and identification of an S-100-like calcium binding
            domain at the amino terminus
  JOURNAL   J. Biol. Chem. (1992) In press
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     protein_bind    1614..1621
                     /bound_moiety="AP1"
                     /standard_name="AP1 site"
                     /gene="FLG"
                     /citation=[3]
                     /note="not known at present if this sequence is bound by
                     thec-fos/cjun/AP1 complex, or whether this sequence
                     isimportant for the upregulation of profilaggrin
                     geneexpression by TPA.; putative"
     exon            1802..1960
                     /number=2
                     /gene="FLG"
                     /evidence="EXPERIMENTAL"
                     /citation=[3]
     intron          1961..2530
                     /number=2
                     /gene="FLG"
                     /evidence="EXPERIMENTAL"
                     /citation=[3]
     exon            2531..4166
                     /number=3
                     /gene="FLG"
                     /evidence="EXPERIMENTAL"
                     /citation=[3]
                     /partial
     intron          order(L01088:314..663,1..1801)
                     /number=1
                     /gene="FLG"
     CDS             join(1823..1960,2531..4166)
                     /gene="FLG"
                     /partial
                     /product="profilaggrin"
                     /codon_start=1
                     /translation="MSTLLENIFAIINLFKQYSKKDKNTDTLSKKELKELLEKEFRQI
                     LKNPDDPDMVDVFMDHLDIDHNKKIDFTEFLLMVFKLAQAYYESTRKENLPISGHKHR
                     KHSHHDKHEDNKQEENKENRKRPSSLERRNNRKGNKGRSKSPRETGGKRHESSSEKKE
                     RKGYSPTHREEEYGKNHHNSSKKEKNKTENTRLGDNRKRLSERLEEKEDNEEGVYDYE
                     NTGRMTQKWIQSGHIATYYTIQDEAYDTTDSLLEENKIYERSRSSDGKSSSQVNRSRH
                     ENTSQVPLQESRTRKRRGSRVSQDRDSEGHSEDSERHSGSASRNHHGSAWEQSRDGSR
                     HPRSHDEDRASHGHSADSSRQSGTRHAETSSRGQTASSHEQARSSPGERHGSGHQQSA
                     DSSRHSATGRGQASSAVSDRGHRGSSGSQASDSEGHSENSDTQSVSGHGKAGLRQQSH
                     QESTRGRSGERSGRSGSSLYQVSTHEQPDSAHGRTGTSTGGRQGSHHEQARDSSRHSA
                     SQEGQDTIRGHPGSSRGGRQGSHHEQSVNRSGHSGSHHSHTTSQGRSDASHGQSGSRS
                     ASRQTRNEEQSGDGTRHSGSRHHEA"
BASE COUNT     1501 a    736 c    851 g   1078 t
ORIGIN      
        1 ggtacctaaa ggcagatgtg agattgaggg tggggtggag atgggaaggt aagtgttaga
       61 tagatatttc agcatgaata tatattgaag ggaatgatcc agtagacaaa gaagaattaa
      121 tgatgcatat gagaaagtag gcaactgcaa aagcaaagtg tttgggtagg caagagagag
      181 tggggctcag tgccaaagta aaggacagga ggagcacaga ctgtttatta ccctaaataa
      241 agtgaaggca aggtatatgg atagaaatgt agtaggttgg gagatcagat attaggagaa
      301 tgaggaaact cttttccaat ggcttccaag ttctcaggaa tcaaaatcaa ggacatcaga
      361 gataaaggaa agaagaagtt gttgaagctt tggccagtgg gtataagaaa aagtaggaca
      421 agtggatgta tccaaggggc atttggggag attaaacata atgtctaacc aaggtaaaaa
      481 gggaagtgag tacataaagc agtaaataga gaggttaaag aattattgac atcagcgtac
      541 aagagaaggt caaagagtga gatccttcag aaagagattt tgcatatgtt gcaagtaacg
      601 ataagttcag agaatacaat atcatcataa ataagactgg ctgaaaataa tcataaactt
      661 taaaatgcat ttgtaagaag catatacctg taatttttta aattcaatct ttacaatcta
      721 tattttaact ggcaagatat ggtctatttt acatgtattg taattagtaa taataaaatt
      781 atttgtaatt ctattaattt attttatgct ttctgtttac ccttcttttt ctatgcttct
      841 tattttcttc tttcctggct tctgttgaat tattaaagtt tcttaattcc cctttttcat
      901 ctctaatggt ttagaaatta taaattctat tattttagta aatacccata aacttaccca
      961 aatacatata tcacttaacc acagcaaaat tcattaatat ctaattcctc ttaccaaaca
     1021 aaccaaggtc aaaaatgttt tagttgtgac tgctacagta atctccttca cattgtttct
     1081 acagtatttt agtttcacct ggttttgtaa gtcctaataa agttataatt ctttatataa
     1141 tacacactta gatttattga catgcttact aatttcttca ccaatgagat aaactgaaca
     1201 ttccttaaat ggcaaaatga aacccagtca gaaaaacctt tctctttaaa tattgaagag
     1261 ctattaaatc catcctatac aatgaaatat tataaacttc atgaaatgtt taaaaaacaa
     1321 ataaaaaatt gaatgaactt tcattgagta tcttgtcata tggctaactg gctttcagag
     1381 aattgtgtct tagcaaatta atcatttatt aagttagctt ttggggaagt aattttcagt
     1441 aaatttgtat aagtaaattt tactgcttaa aaccagtcag cctttctaaa ttcttgttgc
     1501 tagcaatggc aagatcagca agagggatga tcttacctag agcacactga ggtctgtgag
     1561 actactaagt ccagctgtaa gtggcacaag gccaaaaaat gggcaatttt atttaagtca
     1621 ggaaaactct attcatttgt tttatgtaga tggtagagaa aagtttctca tgtccatctt
     1681 ccattcttaa agatggatta cttagaggaa aatgtaggtt taaaatgtgg aaaatgattg
     1741 taataatata acctagaaaa aaagatgtat aaaaaagtgc attttcattt cttctttcta
     1801 ggttcacatt tattgccaaa agatgtctac tctcctggaa aacatctttg ccataattaa
     1861 tcttttcaag caatattcaa aaaaagataa aaacactgac acattgagta aaaaagagct
     1921 gaaggaactt ctggaaaagg aatttcggca aatcctgaag gtaagagtct ctgacaagac
     1981 caaagataga gcatttgttt ttctcatcaa taatctaagc aagggttatt ttgagctctt
     2041 tgtgaactat ttctcttaaa ataggaaaag agttaatctt gtctcaaaag agatagattt
     2101 tattttcttc ccctatacct ggatgtatat tataaacata aagacaccct cagagaaaaa
     2161 aatataaata tttaaaaatt aatgtaaatt agttcttagt attttggtat ttataatatt
     2221 ctcccaaaca tcaacattac taaggggatt tttgttaaag acaactaagt aatgaattat
     2281 atacacttct gacttttaaa ataagaaaag agtcacttac cccatcaaat cttgatacct
     2341 tactagtcta catctttgtt cacatataac acctgaatga ggatgagaca aaaaagtctt
     2401 aaaaaaaaaa aagattttgt cccacttttt aatactcaaa gatcaggttc cttaaattaa
     2461 ctttgaatta attggctgat aatgtgattc tgtctgatgc agtctccctc tgtgacttcc
     2521 ctctgtacag aatccagatg acccagatat ggttgatgtc ttcatggatc acttggatat
     2581 agaccacaac aagaaaattg acttcactga gtttcttctg atggtattca agttggctca
     2641 agcatattat gagtctacca gaaaagagaa tttaccgata tcaggacaca agcacagaaa
     2701 gcacagtcat catgataaac atgaagataa taaacaggaa gaaaacaaag aaaacagaaa
     2761 aagaccctca agtctggaaa gaagaaacaa tagaaaaggg aataagggaa gatccaagag
     2821 cccaagagaa acagggggga aaaggcatga atctagttct gaaaaaaaag aaagaaaagg
     2881 atattcacct actcatagag aagaagaata tggaaaaaac catcataact caagtaaaaa
     2941 agagaaaaac aagactgaaa atactagatt aggagacaat aggaagaggc taagtgaaag
     3001 acttgaagag aaagaagaca atgaagaagg agtatatgat tatgaaaata caggaagaat
     3061 gactcaaaaa tggatacaat caggccatat tgccacatat tacacaatcc aggatgaagc
     3121 ctatgacacc actgatagtc tattagaaga aaacaaaata tatgaaagat caaggtcatc
     3181 tgatggcaaa tcatcatctc aagtgaacag gtcaagacat gaaaatacaa gccaggtacc
     3241 attgcaggag tccaggacaa gaaagcgtag gggatccaga gttagccagg acagggacag
     3301 tgagggacac tcagaagact ctgagaggca ctctgggtcg gcttccagaa accatcatgg
     3361 atctgcgtgg gagcagtcaa gagatggctc cagacacccc aggtcccatg atgaagacag
     3421 agccagtcat gggcactctg cagacagctc cagacaatca ggcactcgtc acgcagagac
     3481 ttcctctcgt ggacagactg catcatccca tgaacaggca agatcaagtc caggagaaag
     3541 acatggatcc ggccaccagc agtcagcaga cagctccaga cactcagcca ctgggcgcgg
     3601 gcaagcttca tctgcagtca gcgatcgtgg acaccggggg tctagcggta gtcaggccag
     3661 tgacagtgag ggacattcag aaaactcaga cacacaatca gtgtcaggcc acggaaaggc
     3721 tgggctgaga cagcagagcc accaagagtc cacacgtggc cggtcagggg aacggtctgg
     3781 acgttcaggg tcttccctct accaggtgag cactcatgaa cagcctgact ctgcccatgg
     3841 acggaccggg accagcactg gaggaagaca aggatcgcac cacgagcagg cacgagacag
     3901 ctccaggcat tcagcgtccc aagagggtca ggacaccatt cgtggacacc cggggtcaag
     3961 cagaggagga aggcagggat cccaccacga gcaatcggta aataggtctg gacactcagg
     4021 ttcccatcac agccacacca catcccaggg aaggtctgat gcctcccatg ggcagtcagg
     4081 atccagaagt gcaagcagac aaacacgaaa tgaggaacaa tcaggagacg gcaccaggca
     4141 ctcagggtca cgtcatcatg aagctt
//