From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: S.cerevisiae gene MAG for 3-methyladenine DNA glycosylase
Date: 25 Jul 92 10:07:33 GMT
Distribution: bionet
Checksum: 30812     4

LOCUS       YSCMAGG      1219 bp ds-DNA             PLN       25-JUL-1992
DEFINITION  S.cerevisiae gene MAG for 3-methyladenine DNA glycosylase
ACCESSION   X57781
KEYWORDS    MAG gene; glycosylase.
SOURCE      Saccharomyces cerevisiae DNA.
  ORGANISM  Saccharomyces cerevisiae
            Eukaryota; Fungi; Eumycota; Hemiascomycetes; Endomycetales;
            Saccharomycetaceae.
REFERENCE   1  (bases 1 to 1219)
  AUTHORS   Chen,J., Derfler,B. and Samson,L.
  TITLE     Saccharomyces cerevisiae 3-methyladenine DNA glycosylase has
            homology to the AlkA glycosylase of E. coli and is induced in
            response to DNA alkylation damage.
  JOURNAL   EMBO J. 9, 4569-4575 (1990)
  STANDARD  full automatic
COMMENT     *source: clone_library=genomic; *source: chromosome=V;
            From EMBL    entry SCMAGG;  dated 25-JUN-1992.
FEATURES             Location/Qualifiers
     promoter        88..101
                     /gene="MAG"
     RBS             121..129
                     /gene="MAG"
     CDS             139..1029
                     /note="allelicto the MMS-5 gene"
                     /product="glycosylase"
                     /gene="MAG"
                     /codon_start=1
                     /translation="MKLKREYDELIKADAVKEIAKELGSRPLEVALPEKYIARHEEKF
                     NMACEHILEKDPSLFPILKNNEFTLYLKETQVPNTLEDYFIRLASTILSQQISGQAAE
                     SIKARVVSLYGGAFPDYKILFEDFKDPAKCAEIAKCGLSKRKMIYLESLAVYFTEKYK
                     DIEKLFGQKDNDEEVIESLVTNVKGIGPWSAKMFLISGLKRMDVFAPEDLGIARGFSK
                     YLSDKPELEKELMRERKVVKKSKIKHKKYNWKIYDDDIMEKCSETFSPYRSVFMFILW
                     RLASTNTDAMMKAEENFVKS"
     polyA_signal    1075..1080
     polyA_signal    1081..1086
BASE COUNT      449 a    186 c    246 g    338 t
ORIGIN
        1 gaattcttac ataaagttta agttatctat gaatcaatga gaattggcca ctgccctctg
       61 atatgacgat ggaagtggta cttttccttt ttaatttttt actgagaatc aagagaagct
      121 agagagggat tggtctcaat gaaactaaaa agggagtatg atgagttaat aaaagcagac
      181 gctgttaagg aaatagcaaa agaattaggg tctcgacctc tagaggttgc tcttcctgag
      241 aaatatattg ctagacatga agaaaagttc aatatggctt gcgaacacat tttagagaaa
      301 gatccatcac tttttcccat acttaagaat aatgaattta cgttgtactt gaaggagact
      361 caagtcccta atacactcga agattatttt attaggcttg caagcacaat tttgtctcaa
      421 cagatcagtg gccaagcagc tgaaagcatc aaggcaaggg ttgtcagtct ttatggcggt
      481 gcatttcctg attacaaaat ccttttcgaa gacttcaaag acccagcaaa atgtgcagaa
      541 atcgcaaaat gtggattgag taaaaggaaa atgatatatc tagagtctct tgctgtctat
      601 tttactgaaa aatataagga tatcgaaaag ctcttcggtc aaaaagataa tgatgaggaa
      661 gtgattgaaa gtttagttac gaatgtaaaa ggtataggcc catggagtgc caaaatgttc
      721 ttgatctccg gattgaaaag aatggatgta tttgctcctg aagatctagg tattgctagg
      781 ggtttttcaa aatacctttc agataagcca gaattggaaa aagaattaat gcgtgaaaga
      841 aaagtagtta aaaagagtaa gattaagcat aagaaataca actggaaaat atatgacgac
      901 gacataatgg aaaaatgctc tgaaacattt tctccgtata ggtctgtgtt tatgttcata
      961 ctttggaggc tcgcgagcac aaatacagat gccatgatga aggcagaaga aaatttcgtg
     1021 aaatcctaac ttaaagatat catgtattac tgacatttat ataagaaaaa aaaaaataaa
     1081 aataaataca ccctattgtt gtgtgtgatg cctactaaag aaattcatat ttacagcttc
     1141 tcgtagggat accaacatat aataagaaaa agcctcacac atacaatcca accattggag
     1201 cgacaaagcg gaaaagcga
//