From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: L. lactis trpE, trpG, trpD, trpF, trpC, trpB trpA genes, complete
Date: 31 Dec 92 01:45:55 GMT
Distribution: bionet
Checksum: 18185 18

LOCUS       LACTRPOP     8656 bp ds-DNA             BCT       18-DEC-1992
DEFINITION  L. lactis trpE, trpG, trpD, trpF, trpC, trpB trpA genes, complete
            cds.
ACCESSION   M87483
KEYWORDS    N-phosphoribosyl anthranilate isomerase;
            anthranilate synthase alpha subunit;
            anthranilate synthase beta subunit;
            anthranilate synthase component I;
            anthranilate synthase component II;
            indoleglycerol phosphate synthetase;
            phosphoribosyl anthranilate transferase;
            phosphoribosyl transferase; tryptophan synthase alpha subunit;
            tryptophan synthase beta subunit.
SOURCE      Lactococcus lactis (strain IL1403, sub_species lactis) DNA.
  ORGANISM  Lactococcus lactis
            Prokaryotae; Firmicutes; Firmibacteria; Gram-positive cocci;
            Streptococcaceae.
REFERENCE   1  (bases 1 to 8656)
  AUTHORS   Bardowski,J., Ehrlich,S.D. and Chopin,A.
  TITLE     Tryptophan biosynthesis genes in Lactococcus lactis subsp. lactis
  JOURNAL   J. Bacteriol. 174, 6563-6570 (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     -35_signal      515..520
     promoter        515..542
                     /label=p1
     -10_signal      537..542
     misc_structure  781..822
                     /standard_name="anti-terminator"
     terminator      808..837
                     /label=t1
     -35_signal      821..826
     promoter        821..850
     -35_signal      842..847
     promoter        842..870
                     /label=p3
     -10_signal      845..850
     -10_signal      865..870
     terminator      6271..6309
                     /label=t2
     -35_signal      6396..6401
     promoter        6396..6423
                     /label=p4
     -10_signal      6418..6423
     terminator      8594..8634
                     /label=t3
     CDS             137..496
                     /note="ORF 1"
                     /codon_start=1
                     /translation="LTILTIILSLLVALEFFYIMYLETFATSSKTTSRVFNMGKEELE
                     RSSVQTLFKNQGIYNGLIGLGLIYAIFFSSAQLEIVRLLLIYIILVALYGSLTSNKKI
                     ILTQGGLAILALISSFF"
     CDS             954..2324
                     /EC_number="4.1.3.27"
                     /gene="trpE"
                     /evidence="EXPERIMENTAL"
                     /product="anthranilate synthase alpha subunit"
                     /codon_start=1
                     /translation="MRKIKEISADTMTPISVYLRLKGKNKVILESIPRENDQSRFSII
                     ALNPVKHIKFTDGILSVNDEIISDENPMEFLEKLVCQPESTDENLDLPFTSGAIGYAG
                     FDTYGIFEGIQPELKDSIGTPDMYFMLYESALIFDHKREKLIFIEDNTYSQRSEKELQ
                     NALYRNIESLSLLTEAENELTELSKLNFVSNMSQELFEEKVAKAKELIRNGDMFQVVL
                     SQRLTADFTDNPFNYYRKLRVENPSSYMYFMEFDNFHVIGSSPERLVAVHGNQVSTNP
                     IAGTRKRGQTEFEDQALIEDLESDPKEVAEHKMLVDLGRNDIGKISKYGSIEVPVFMK
                     VEKYRYVMHITSEVTGELRPEFTAMDALRATLPAGTLSGAPKHRAYQRIYEFETQKRG
                     IYGGAIGYLTKNGNCDFAIAIRTMVLKDKKAHVQAGAGIVYDSVPEHEYQETLNKAQG
                     LLKVGQ"
     CDS             2449..3045
                     /EC_number="4.1.3.27"
                     /gene="trpG"
                     /product="anthranilate synthase beta subunit"
                     /codon_start=1
                     /translation="LILIIDNYDSFTYNLVQYVGVLTDVAVVKNDDDSLGNMAEKADA
                     LIFSPGPGWPADAGKMETLIQQFAGQKPILGICLGFQAIVEVFGGKLRLAHQVMHGKN
                     SQVRQTSGNLIFNHLPSKFLVMRYHSIVMDEAVALPDFAITAVATDDGEIMAIENEKE
                     QIYGLQFHPESIGTLDGMTMIENFVNQVNENKESSNEK"
     CDS             3035..4042
                     /EC_number="2.4.2.18"
                     /gene="trpD"
                     /product="phosphoribosyl anthranilate transferase"
                     /codon_start=1
                     /translation="MKNELEKVMSGRDMTENEMNMLANSIIQGELSEVQIASFLVALK
                     MKGEAASELTGLARALQKAAIPIPTNLTNAMDNCGTGGDRSFSFNISTTAAFVLAAGG
                     VNMAKHGNRSITSKSGSADVLEALGINLYLPAEKLAQVFDKVGLVFLFAQNLHPAMKY
                     FTPVRRQLEIPTIMNLTGPLINPIPLDTQLLGTSRPDLLELTANVLKGLGRKRALVIT
                     GEGGMDEATPFGLNHYALLENDKVTLHEFRASEVGISEVQLNDIRGGEAPENAEILKN
                     VLENQPSAFLETTVLNAGLGFYANGKVDSIKSGVDLAREVISTGAALTKLHELQAEQI
                     G"
     CDS             4089..4883
                     /EC_number="4.1.1.48"
                     /gene="trpC"
                     /product="indoleglycerol phosphate synthase"
                     /codon_start=1
                     /translation="MNIKKGKFLETILAEKRLEIAKMPEEQVGKVRQTYNFYDYLKEH
                     SDQLQVIAEVKKASPSLGDINLEVDIVDQAKNYEQAGAAMISVLTDPVFFKGNIEYLC
                     EISENVQIPTLNKDFIIDKKQINRAVNAGATVILLIVAVFENQYPKLQNLYNYALSLG
                     LEVLVETHNKAELEIAHQLGAKIIGVNNRNLKTFEVILQNSVDLTPYFKEDSIYISES
                     GIFSANEAQKVSDTFNGILVGTALMQSENLEKSLKDLKVKRKTNEN"
     CDS             4873..5928
                     /gene="trpF"
                     /product="phosphoribosyl anthranilate isomerase"
                     /codon_start=1
                     /translation="MKIKICGLSTKEAVDTAVESGVTHLGFILSPSKRQVAPEKILQI
                     TNDVPKTVKKVGVFVDEPIDFVKKAIQVAQLDLVQLHGNEDMNYINQLDISVIKAIRP
                     DQEFKEYEDVILLFDSPQAGSGQAFDWDSLVTSGLKNKFFIAGGLNPENVAAAIQHFP
                     NAYGVDVSSGVETDGIKNLTKIKNFVQNASLASSKQLFIEFLRITKKLNENKIIPYLM
                     GSLAVEQIINFPTNPDDIDIQLKTSDFENFEQLTSLMEKLGYQLIDLHEHKFEKASIH
                     VGFASVETLKNYAGVDYLTIQQERMENGEKYHLPNVEQSLKIYEAAKRDEWRGGKQKD
                     SFIFDELIKEQKRNDNE"
     CDS             6514..7722
                     /EC_number="4.2.1.20"
                     /gene="trpB"
                     /product="tryptophan synthase beta subunit"
                     /codon_start=1
                     /translation="MTYNQPNNKGFYGQFGGQFVPETLMTAVKQLEEAYVDSKKDPLF
                     QAELKELLKDYVGRENPLYYAKRLTEYAGGAKIYLKREDLNHTGAHKINNALGQVLLA
                     KKMGKNKVIAETGAGQHGVASATAAALFGMECTIYMGEEDVKRQSLNVFRMELLGAKV
                     HSVTDGSRVLKDAVNAALRAWVAQVEDTHYVMGSVLGPHPFPQIVRDYQAVIGQEARA
                     QFLEKENKLPDALVACVGGGSNSMGLFYPFVNDESVAMYGVEAAGLGIDTPHHAATIT
                     KGRPGVLHGTLMDVLQDENGQMLEAFSISAGLDYPGIGPEHSYFNAVGRAKYVDITDE
                     EALEGFKILSRTEGIIPALESSHAIAYAVKLAKELGADKSMIVCLSGRGDKDVVQVKE
                     RLEAEKEVKK"
     CDS             7719..8480
                     /EC_number="4.2.1.20"
                     /gene="trpA"
                     /product="tryptophan synthase alpha subunit"
                     /codon_start=1
                     /translation="MKTLQMTLSNKKNNFIPYIMAGDHEKGLEGLKETIQLLEQAGSS
                     AIEIGVPFSDPVADGPVIEQAGLRALARNVSLSSILETLKTIDTKVPLVIMTYFNPVY
                     QFGIEKFVAALEKTPVKGLIIPDLPKEHEDYIKPFINDKDICLVPLVSLTTPLSRQKE
                     LVADAEGFIYAVAINGVTGKENAYSNQLDQHLKALSSLTDVPVLTGFGISTLSDVDRF
                     NKVSSGVIVGSKIVRDLHEGKENEVIKFIENAINF"
BASE COUNT     3022 a   1456 c   1671 g   2507 t
ORIGIN      
        1 aagctttcga tttagacaaa gaacgttttg tcccagttat gattttgtca attggtaaag
       61 ccgttgaaga aggttatgaa tctgtccgca tgaatgctga taaaatcact acttttaaat
      121 aaaaaggaga agtaatttga ccattttaac aattattctg tcgcttttgg ttgctttgga
      181 atttttctac attatgtatt tagagacctt tgctacaagc tcaaaaacaa ccagtcgtgt
      241 gtttaatatg ggaaaagagg agctagaacg ttcttcagtt caaacgcttt ttaaaaatca
      301 agggatttat aacggtttga ttggtttagg attaatttat gcaatattct tttcgtcagc
      361 acaacttgaa attgttagat tactacttat ttatattatt ttagtggctt tgtatggctc
      421 tttgacaagt aataaaaaga tcattctcac ccaaggtggt ttagccattt tagctctaat
      481 ttcttcattt ttttaaaaaa atatttttaa atccttgaca agtctcataa aaagttttac
      541 aattattttc aacatcagaa aagtaactct atatcagaat tttttcagga agtctgcggt
      601 tgtgcgagca gataaattca tcagagcgaa tgggctgatt tgaaagtcga gaacgatttg
      661 tttttcgcaa tttgatttga aacaataaaa atcacggatg ctcccgatac caagcgactt
      721 gttgttagac gaggaaaaga agagtttttc tagtattttt ttaatacttt tagggactct
      781 taaattacgg tggcaccgcg tgacatacgc ccgtagagta tttgatactc tacgggcttt
      841 tttgtatttt tttgaaagga tttctaaaat gaaccaacga tggcgaagct taaattttaa
      901 ctaatcagta tcaatggaaa agactacctc aaacctatag atagagagaa aacatgagaa
      961 aaattaaaga aatttcagca gacaccatga ccccgatttc ggtttatctt cgcctgaaag
     1021 ggaaaaataa agtgatttta gaatccattc ccagagaaaa tgaccaatca cgtttttcaa
     1081 ttattgcgtt gaatcctgtg aagcacatca aatttactga cggaatttta agcgtaaatg
     1141 acgaaatcat tagcgatgaa aatccaatgg aatttctgga aaaattagtt tgtcagccag
     1201 aaagtactga cgaaaattta gacttgcctt ttacaagtgg agcgattgga tatgctggat
     1261 ttgataccta tggtattttt gaaggcattc aacctgaact gaaagattcg attggaacac
     1321 cagatatgta ttttatgtta tacgaatcag ctctaatttt tgaccacaaa cgagaaaaac
     1381 tgatttttat tgaagacaat acttacagtc agcgttcaga aaaagagtta caaaacgcgc
     1441 tgtacagaaa tattgagtca ttatctttac tgacagaagc agaaaatgaa ctgacagaat
     1501 taagtaaact gaatttcgtc agcaatatgt ctcaagaact ttttgaagaa aaagttgcta
     1561 aagcaaagga acttatcaga aatggtgaca tgttccaagt tgttttatca caacgtttaa
     1621 cagccgattt tactgacaac ccttttaatt attacagaaa gcttcgggtt gaaaatccgt
     1681 catcgtatat gtactttatg gaattcgata attttcatgt cattggcagc tcaccagagc
     1741 gtttggttgc agttcatgga aatcaagttt caaccaatcc aattgccgga acaaggaaac
     1801 gcgggcaaac tgaatttgaa gaccaagctc ttattgagga tttagaaagt gatcccaaag
     1861 aagtcgctga acacaaaatg cttgttgatt taggacgaaa tgatattggt aaaatttcca
     1921 aatatggttc aatcgaagtt cctgttttca tgaaagttga gaaatatcgc tacgtcatgc
     1981 atattacaag tgaagtgacc ggcgagcttc gtcctgaatt tacagctatg gatgccttac
     2041 gcgccacatt gccagcgggg acgctatcag gagcaccgaa gcatcgagcc taccaaagaa
     2101 tttatgaatt tgaaacgcaa aaacgaggaa tttatggtgg agctatcggt tatctaacga
     2161 aaaatggaaa ttgcgatttt gcaattgcca ttcggacaat ggtcttgaaa gacaagaaag
     2221 cacatgttca agcaggagcc ggaattgttt acgattcagt acccgaacat gaatatcaag
     2281 aaaccttaaa caaagcccaa ggcttattga aagttggtca ataaatagac ccaaaaagat
     2341 gtagagataa aaaatttaac atcatcagat aattttgtca gtaatttttt actgacagaa
     2401 aataaaaaat tactgacagg ccttgtcgtt aaaaaatagg agacaaaatt gattttaata
     2461 atagataatt acgatagttt cacttacaac ttagtgcagt atgtaggtgt tttgactgat
     2521 gtagcagttg tgaaaaatga cgatgattct ttgggaaata tggcagaaaa agcagatgcc
     2581 ttaatattct caccaggccc tggttggcct gcggatgctg gaaaaatgga aacacttatt
     2641 cagcaatttg caggtcaaaa accaatttta ggaatatgtc taggttttca agctatcgtt
     2701 gaagtttttg gtggcaaact tcgattggca catcaagtca tgcatggcaa gaattcacaa
     2761 gtccgtcaga cctcaggaaa tttaattttc aatcatcttc cctcaaaatt tctagttatg
     2821 cgctatcatt caattgtcat ggatgaggca gtcgctttac cagattttgc aatcacagca
     2881 gtcgcgacag atgatggtga aattatggct attgaaaatg aaaaagaaca gatttatgga
     2941 ttacaatttc accccgaatc cattggcaca ttggatggta tgaccatgat tgaaaatttt
     3001 gtcaaccaag taaatgaaaa caaggagtca tcaaatgaaa aatgaacttg aaaaagtgat
     3061 gtcaggtcgt gacatgaccg aaaatgaaat gaatatgctt gctaattcaa ttatccaagg
     3121 tgaattaagc gaggtccaaa ttgccagctt tttagtagca ttaaaaatga aaggtgaagc
     3181 agcaagcgaa ttgactggtt tggctcgagc tttacaaaaa gcagcgattc ccattccaac
     3241 aaatttgaca aatgcgatgg acaattgtgg aacaggaggc gaccgctcat tcagttttaa
     3301 tatttcaacc acagccgctt ttgttttagc agctggtgga gtcaatatgg caaaacacgg
     3361 aaatcgctcc attaccagta aatctggctc ggcagacgtt cttgaggcct taggaatcaa
     3421 tctttattta ccagcagaaa agttagctca agtttttgac aaagttggtt tagttttcct
     3481 ttttgctcaa aatctccacc cagcgatgaa atacttcacg ccagtccgca gacaactcga
     3541 aattccaaca attatgaact tgactgggcc actaatcaat ccaattccac ttgatacgca
     3601 acttcttggt acctcacgtc cagatttact tgaattaaca gcaaatgttt tgaaaggctt
     3661 gggccgtaag cgagcattag tcatcacagg tgaaggcgga atggacgaag caactccctt
     3721 tggacttaat cattacgcac ttttagaaaa tgacaaagtg actttgcatg aatttagagc
     3781 ctcagaagtt ggtatttcag aagttcaact caatgatatt cgtggaggtg aagccccaga
     3841 aaatgctgaa attttaaaaa atgtccttga aaatcaaccg tcagcctttt tagaaacgac
     3901 cgttttaaat gccggacttg gattttatgc caatggaaaa gttgattcca tcaaatccgg
     3961 agttgacctt gcaagagaag taattagtac aggagcagct cttaccaagt tgcatgaatt
     4021 acaagcagaa caaattggtt aaaaatcttg atggcaaatt ttgaaatagc agaaaatgag
     4081 agaaaaatat gaacataaaa aaaggaaaat ttctagaaac aatcctagca gaaaaacgac
     4141 ttgaaattgc taaaatgcca gaagaacaag taggaaaagt tcgtcaaaca tacaattttt
     4201 atgattactt aaaagaacat tccgaccagc ttcaagtgat tgccgaagtc aaaaaagctt
     4261 cgcccagtct aggtgatatt aacttagaag tggatatcgt tgaccaagcc aaaaattacg
     4321 aacaagccgg tgccgctatg atttccgtct taactgaccc tgtatttttt aaaggaaata
     4381 ttgaatatct ctgtgaaatt tcagaaaatg tccaaatccc caccttgaac aaggatttta
     4441 tcatcgataa aaaacaaatc aatcgggcag ttaatgcggg agcaacagtt attttactca
     4501 ttgtcgcagt ttttgaaaat caatacccca aactccaaaa cctctacaac tacgcacttt
     4561 cactaggact tgaagttctt gttgaaacac ataataaagc agaacttgag attgctcatc
     4621 agcttggagc taaaattatt ggagttaata atcgtaattt aaaaaccttt gaagtgatct
     4681 tacaaaattc agtagatttg acaccctact ttaaagaaga cagtatctac atttccgaat
     4741 caggcatttt tagcgcaaac gaagcccaaa aagtttccga tactttcaat ggaatattgg
     4801 ttggaacagc actcatgcaa tcagaaaatc tagaaaaatc tttgaaagat ttaaaagtca
     4861 agaggaaaac gaatgaaaat taaaatctgt ggcttatcta caaaagaagc tgttgataca
     4921 gctgtagaat ctggtgtcac acatctcggt tttattctta gtccctcaaa acgccaagtt
     4981 gcaccagaaa aaattcttca aatcacaaac gatgtcccaa aaacagtcaa aaaagtagga
     5041 gtttttgttg atgaacctat tgattttgta aaaaaagcca ttcaagttgc tcaactcgat
     5101 ctggttcagc ttcacggaaa tgaagatatg aattacatta atcaactaga tatttcggtt
     5161 attaaagcaa taagaccaga ccaagaattt aaagaatacg aagatgtaat tttattattt
     5221 gatagtccac aagctggaag tggtcaagca tttgattggg actctttggt gaccagcggt
     5281 ctgaaaaata aatttttcat cgctggtgga cttaatccag aaaatgtagc agctgctatt
     5341 caacattttc caaatgccta cggtgtggat gtttcttctg gagtagaaac tgacggaatt
     5401 aaaaacctta caaaaataaa aaactttgtt caaaatgcaa gccttgcctc atcaaagcaa
     5461 ttatttatag aatttttaag aatcacaaaa aagctaaatg aaaataagat tatcccttat
     5521 ttaatgggaa gtttagcagt tgagcaaata atcaattttc caacaaatcc tgatgacatt
     5581 gatattcaac tcaaaacgtc tgattttgaa aattttgagc aattaacaag tttaatggaa
     5641 aaattaggtt atcagcttat tgacttacat gagcataaat ttgaaaaagc tagtattcat
     5701 gttggctttg caagtgtgga gacccttaaa aactatgccg gggttgacta tttgaccatt
     5761 caacaagaaa gaatggaaaa tggcgaaaaa tatcatcttc caaatgttga acaatccctt
     5821 aaaatctatg aggcagcaaa acgagatgag tggcgaggag ggaagcaaaa agattccttt
     5881 attttcgatg agttaataaa ggaacagaag aggaatgaca atgaatgata atcttattga
     5941 agagggtgta gagattcgaa atggtctcat tattaagtca attcaaaaag aagatatatt
     6001 agagctttgg caaattagtt atggacctaa atctgattta cattggatgt ctttcaacgc
     6061 tccctatttt gaggagccaa tcctgagttg ggaagaattt tcaagaaaaa tatctcttaa
     6121 aataaattaa ccaaatgttg cacttattat ctttcaaaat cgaatcattg gaatgctgtc
     6181 agcttattgg gaagacggta aattacaaaa atggcttgag tttggtatag tgatttatga
     6241 tagtaaattg tggggacgtg gaattggaca ggatgcctta tctttttggt tgaagcacct
     6301 ttttgaaact tatccgaaga ttcagcacat aggatttaca acttggtcag gaaatcaagg
     6361 aatgatgaga ctaggagaaa aaagtggtct aaaacttgaa gggcaaatca gaaaagttag
     6421 atattggcaa gaaacttggt atgattcaat aaaatatgga attttaagag aagaactaaa
     6481 aaaataaata aaaaaaatca aaggagcaac aacatgacct acaaccaacc taacaacaaa
     6541 ggattttacg gccaattcgg gggccaattc gtacctgaga cactaatgac agcagtaaaa
     6601 caattagaag aagcctacgt agatagtaaa aaagaccctc tctttcaagc agaacttaaa
     6661 gaattactta aagactatgt tggacgagaa aacccactct attatgcaaa acgcttaaca
     6721 gaatatgcgg gcggagcaaa aatttatctt aaaagagaag acctaaacca tacaggagca
     6781 cacaaaatta acaatgccct cggacaagtc ctccttgcca aaaaaatggg aaaaaataaa
     6841 gtcattgctg aaacaggtgc aggccaacac ggtgtcgcaa gcgcaaccgc ggctgccctc
     6901 tttggcatgg aatgtacgat ttatatgggt gaagaagacg ttaaaagaca atctctcaat
     6961 gtctttcgca tggaattact cggggcaaaa gttcattcag taactgatgg ttcacgcgta
     7021 cttaaagatg cggttaatgc agcacttaga gcatgggttg ctcaagttga agatacgcat
     7081 tatgtaatgg gctcagttct tggaccacat ccatttccac aaattgtgcg tgattatcaa
     7141 gctgttattg gacaggaagc gcgtgcccaa tttttagaaa aagaaaataa acttccagat
     7201 gctttagtag cttgtgtcgg tggaggttca aattctatgg gactttttta tcccttcgtt
     7261 aatgatgaat cagttgccat gtatggtgtt gaagccgctg gccttgggat tgatacacca
     7321 catcatgcgg caacaattac taaaggccgc cccggtgttc ttcacggaac actcatggat
     7381 gtccttcaag atgaaaatgg tcaaatgtta gaagccttta gtatttcagc cggtttagac
     7441 tatccaggaa tcggaccaga acactcttat ttcaatgctg ttggacgagc aaaatatgtt
     7501 gatattacag atgaagaagc acttgaaggt tttaaaatct tatctagaac tgaaggaatt
     7561 atcccagcac tagaaagttc tcatgctatc gcctatgcag tcaaattagc aaaagaatta
     7621 ggagcagata aatcaatgat tgtttgtctt tcaggacgtg gagataagga tgtggttcaa
     7681 gttaaagaac gacttgaagc agaaaaagag gtgaaaaaat gaaaacttta caaatgactt
     7741 taagcaataa aaaaaataat tttattcctt atatcatggc tggcgaccat gaaaaaggct
     7801 tagaaggtct taaagaaacc attcaactgc ttgagcaagc tgggagttcc gctattgaaa
     7861 ttggcgttcc attttcagat ccggttgctg atggtccagt catcgaacaa gcaggtttgc
     7921 gtgcgttagc aagaaatgta tcactttcaa gtattcttga aaccttaaaa acaattgata
     7981 caaaagttcc tctagtaatt atgacctatt tcaatcccgt ttatcagttt ggaattgaaa
     8041 agtttgttgc agctcttgaa aaaacaccag ttaaaggcct tatcattcct gatttgccta
     8101 aagaacatga ggactatatc aaaccattta tcaatgataa agatatctgt ttagttcctc
     8161 tggtctcatt aaccacgcca ctttctcggc aaaaagaact tgtagccgat gctgaaggat
     8221 ttatctatgc cgttgcaata aatggagtaa ctgggaaaga aaatgcttat agtaaccagc
     8281 ttgaccaaca tttaaaagcg ttatcttcat taacggatgt tcctgttttg acaggatttg
     8341 gaatttctac attatctgat gtggaccgtt ttaataaagt gtcctcagga gttattgttg
     8401 gttcaaaaat tgttcgtgat ttacatgaag gtaaagaaaa cgaagttatt aaatttattg
     8461 aaaacgcaat caatttttaa gataattgaa aaattaataa aaccatatat ccttgacagt
     8521 ccactttaca aatggtatac ttttattaaa taaaacggtt acaaaaaatt ggatagtgat
     8581 tattaagtta agctagacct tatcaaacct atagttatag ggggataagg tctatttttg
     8641 tgaaatggag aagaat
//