From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Murine cytomegalovirus e1 protein gene, complete cds.
Date: 29 Dec 92 00:30:16 GMT
Distribution: bionet
Checksum: 34146 10

LOCUS       HS5E1A       4536 bp ds-DNA             VRL       03-DEC-1992
DEFINITION  Murine cytomegalovirus e1 protein gene, complete cds.
ACCESSION   L07320
KEYWORDS    e1 protein.
SOURCE      Murine cytomegalovirus (strain Smith) (library: ATCC VR-194) DNA.
  ORGANISM  Murine cytomegalovirus
            Viridae; ds-DNA enveloped viruses; Herpesviridae;
            Betaherpesvirinae.
REFERENCE   1  (bases 1 to 4536)
  AUTHORS   Buehler,B., Keil,G.M., Weiland,F. and Koszinowski,U.H.
  TITLE     Characterization of the murine cytomegalovirus early transcription
            unit e1 that is induced by immediate-early proteins
  JOURNAL   J. Virol. 64, 1907-1919 (1990)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     mRNA            join(1732..2643,2737..2913,3240..4259)
                     /function="e1 mRNA"
                     /standard_name="e1 gene"
                     /gene="e1"
                     /evidence="EXPERIMENTAL"
                     /label=e1_mRNA
                     /citation=[1]
     promoter        1..1731
                     /function="promoter upstream region"
                     /standard_name="e1-promoter"
                     /gene="e1"
                     /evidence="EXPERIMENTAL"
                     /note="inducible by the MCMV immediate-early protein 3"
     old_sequence    replace(1689..4278,"")
                     /citation=[1]
                     /note="recently annotated under accession number M35146.
                     Thissubmission contains additional upstream promoter
                     sequences.M35146 can be substituted by the new entry.; ''
                     in old_sequence"
     TATA_signal     1704..1708
                     /gene="e1"
                     /citation=[1]
                     /note="putative"
     exon            1732..2643
                     /number=1
                     /standard_name="e1 gene"
                     /gene="e1"
                     /evidence="EXPERIMENTAL"
                     /citation=[1]
     intron          2644..2736
                     /number=1
                     /gene="e1"
                     /evidence="EXPERIMENTAL"
                     /citation=[1]
     exon            2737..2913
                     /number=2
                     /standard_name="e1 gene"
                     /gene="e1"
                     /evidence="EXPERIMENTAL"
                     /citation=[1]
     intron          2914..3239
                     /number=2
                     /gene="e1"
                     /evidence="EXPERIMENTAL"
                     /citation=[1]
     exon            3240..4259
                     /number=3
                     /standard_name="e1 gene"
                     /gene="e1"
                     /evidence="EXPERIMENTAL"
                     /citation=[1]
     polyA_signal    4207..4213
                     /gene="e1"
     polyA_signal    4236..4241
                     /gene="e1"
                     /citation=[1]
                     /note="putative"
     polyA_signal    4259..4264
                     /gene="e1"
                     /citation=[1]
                     /note="putative"
     CDS             join(1851..2643,2737..2913,3240..3262)
                     /gene="e1"
                     /label=E1
                     /citation=[1]
                     /note="putative"
                     /product="e1 protein"
                     /codon_start=1
                     /translation="MAAPDRRGSPIVSRRRYFTFRNSSRTLHQNVTRMFDLRQWTYES
                     ARVLDCTNGEGRTENWGPGWLCATIMQSPDSGSSSGSAAQGCMSLDITSDDGPEKVMN
                     MFHRGSIVCNKTVSSVATPPGSDGTGASLLTLVADGSLLQVILVEHSPGAHRAEADDV
                     AESVGNAAVAAAVQSATSMGSSSSVGGAGGGGFNSSEERRRDKDSSKSHDDDRRKRFE
                     LCNGTLHRERTSGSSGSSGGGGGSGSGGSSGLSTKQKERSRRLEEECSPRSGGEPKRQ
                     KTHHDPRPERDLEPPRSSTTVDGNADEAGAVSFLNSYAASSLSAVSDGGQQPAF"
BASE COUNT      969 a   1261 c   1333 g    973 t
ORIGIN      
        1 tctagagaga atagagcgaa aatgatttac ctagtatcta gtttcatgtt tgcatattag
       61 tctattgtat aatattgtgt ttattatata tctgcaacag aacagaaaga agtttgatgt
      121 agtttggttt ggaattagtt tctttaattt tttcagactc tacctagcca ggattcctag
      181 gccgattgga gaaaaaatga gatctacgct cgaccgaggc cgagtgaaaa aagtctttta
      241 tagctttatg gaagatatag tacctctaac atagaaaaaa agtattctgt ttttcgggtt
      301 tttatgaatt accatctaaa ggaacgataa aagaggacag tagataaaga ttgatttagt
      361 gtacacacgt tgttcctacc tgagagctcc gggccttcgg gacgccgtca cctccgccgc
      421 ctcctcggcc tccgggagat gcgtccgccg cctcacctga gcctgctcgg ccgttcgctc
      481 aggtgtgata atgcaccttt cagtaacacc gtacgtttat atattctagc cgggatgcgt
      541 caggttgcgt ctgacggtga atacctacac tccaggtatc ctacctagcg cggtcacgtc
      601 cgacgccgca aagtataaca gcggagtacg gagacacacg gtggtagcgt gatcatttta
      661 tttccccgtg ccggtggggt tccttcggcc gacccggtca cggactttct ttttaacccg
      721 ggggctggat gggcgcatgg acggacggac ggacgggcgg gcgggcggac ggggacgggt
      781 tcggagggag tacgtaggga ggtgctccat cggcggcctg accgacgctc gtcgaccggt
      841 cactcgtctc gagtcaccgt ccgtagcgac tcggtgctcg cgtcgagtga ccgctcgcga
      901 cgagtgatct ctcgcttcga ccgacccctc gtagcgaccg accgctcgct tcgagtaccc
      961 gctcgtccgt gttatttgat tgccgcgaac gccacgaccg ctcgatcgaa gcgaacgggt
     1021 gctcgtacca cgtccgcgtg tccgatgcga tcaggtaacc gaagcggacc tgaaaccggg
     1081 gaaggtccgt cagccctcgg aggatcggac gaccgctcga cacgagccgc cgtcagaaac
     1141 gaacggagct ccgtacgccg tatacttaca cgtgcgtaac gaggcggaag acccacattt
     1201 cgggcggttg ctcgtgaacg agcggtcgct cgtcacggcg ataccgacgg tgctcgaccc
     1261 cctcatcgag ccgatcggac cgggaaaacc cctcttcgga cacccgctcg acgccaccgg
     1321 catctcgaac gccgtacggt gcgacggacg ccgcgctatc tcgagccgag gcccctgcgc
     1381 ttcggcgaag gcctcgcgcg gccggccagg acgaggagcg ccactaggtt gaacatccgc
     1441 acgagccgcc gggccaggtc tcggacgggc tctcgagact cgatctcgtg catgtcggcg
     1501 gtccgcggtg aggttataga ccatctgcta ggcgggtccg gggagacagg cacattactg
     1561 gcctcggcgc ccagcctagg cgtgtctaga gctcgaccgc gcgtccggag cgccattcga
     1621 ccggcgggta gcgagaagaa cgccggagac cgcaggttat aacaacgtca tgcataaatt
     1681 aagaatgggc gtgatgcaga ctttataaat cgcaagccgg agcggatcct aggctcattt
     1741 cttctgcgat cttcgcattt agcagaccaa atgctgatag ttcctgcgtc gcggtagatt
     1801 acgtgcccac ttttctcgtc gcgaccggtg aaaagacctt cgttcggacc atggccgcgc
     1861 cagatcgacg cggatcgccc atcgtttcga gacgacgtta cttcaccttc cggaactcga
     1921 gtcggacgct gcatcagaat gtcacccgca tgttcgacct ccgtcagtgg acctacgaga
     1981 gcgcccgcgt tttggactgc acgaacggcg agggccgcac cgagaactgg gggcccggct
     2041 ggctgtgcgc gaccatcatg cagtctccgg acagcggctc gtcgtccgga tcggcggccc
     2101 agggatgcat gtctctggac atcacctcgg acgacggacc ggagaaggtc atgaacatgt
     2161 tccaccgcgg gagcatcgtc tgtaacaaga ccgtctcttc ggtcgcgacg ccgccggggt
     2221 ccgacggcac gggcgccagc ctcttgactc tggtggcgga cggcagcttg ctgcaagtca
     2281 tcctggtcga acactcgccg ggcgcccacc gcgccgaagc cgacgatgtc gccgagagcg
     2341 tcggcaacgc cgcggtggcc gccgccgtgc agtcggccac ttcgatgggt agctcgagct
     2401 ccgtcggcgg cgccggtggc ggcggcttca attcttcgga agaacgtcgc cgcgataaag
     2461 actcgtccaa atctcacgat gacgaccgac gtaagagatt cgaactctgc aacggtaccc
     2521 ttcataggga gcggacctcg ggtagctccg gatcgagcgg cggcggcggc ggcagcggca
     2581 gcggaggcag cagcggcctc agtacaaagc agaaggagcg cagcaggagg ctcgaagagg
     2641 aatgtaagta gttctgatcg gggttttact ggtcgctttc cccctcgacc ttcggtgaac
     2701 cgtcacggga ctaatgttgt tactctgttc ggaaaggttc tccacgcagc gggggggagc
     2761 ccaagcgcca gaagacccac cacgatccga gaccggagag ggatctggaa ccgcctagga
     2821 gcagtaccac tgtcgatggt aatgcagacg aagctggagc ggtctccttt ttgaacagtt
     2881 atgctgctag ttcgctgtcc gccgtcagcg atggtgcgtt accaccctcc tctccggtga
     2941 ctatagagac accaaggttt aatgacttga tagaatccga ggaggaagac gatggaactc
     3001 ggtgttcgtc tgccgatggt acggcgacgg taaccgagac cggggcgtct gccgagatcg
     3061 agcaaacgtt caccgacggt gggctagcat gcatgaggtc cttaattgac tcgcgggccc
     3121 tcatggatct cgcctttacg agccgcatga cggcggtgaa gtcgggatct gagggagcgg
     3181 gcaacacctg tcctggggaa cagaggcttt ctaactgcaa tcttcctccg aattcacagg
     3241 aggacaacaa cccgcgtttt gaaggcgtct ttaattccct gatgagactc ttagacgagt
     3301 ataaagacaa gagcaccagc tcgagttccg ccgccgtacc gggatccagt tgtacaggca
     3361 ccacgcagac agaggggatg cctcaccaca ggggtatctt cgacagggac acatccgccg
     3421 ctcagcagaa acctgtatgt gagatacggc ccttcatcga catagctcgc tgtgtcgagc
     3481 cggcggcggc gccgaccgct tcgcgctcca gagccaccac ggccgccgct cgcaggcggg
     3541 gaagaggctc gcagcctcgg aggaacagtc gggtcgcggc gcgcgtcgcc caggtcgatc
     3601 cgaccgaccc acccgcagct gctgctgccg ccgccgccgg tcccaccacg cggggaagga
     3661 gcaggagtag gaggggaaga cgaggcccag acctcacgga ggacgggttg gagattgtcg
     3721 agacgggcgc caccgccggc actctggccg tttccgagga ggaaaccgcg atggccgccg
     3781 ctatgttaga ggatatggtc gacttggaca atgtgttcga tgatcttaat tgatctcatg
     3841 aaagatggat aaagaaatga tgctctgtaa actctaactg actgttgtta tcgatcgcct
     3901 cgtctcgctc gggtcttctg ccgatgctgc cgtctgtaag cacgcaagca agcactcgct
     3961 ctatagcagt agaatatgta tggatgatat acttactgtt gttgttcact tactattatc
     4021 acctatataa aacttgtatt catgatactt accactgaaa aaattgtgta tgtcttttta
     4081 tacttttgac caccgttatt gatgataagt atactcttct aactgtgtgt gtctctgtct
     4141 accgggccga tggagatatt atccctggtc cccctctgtc tgtgctaaca acataacatc
     4201 catgtaaata aaattaatat ttttaatatt ttatcaataa aaaccacaca tttgttacaa
     4261 taaaccgcgc tcgcctttat tttttgtgtt tggtggtgga gttggaggag gttgtggggg
     4321 agataataat attattctca tcggtggtta cttcgaacac agcacgttcc agtcgacgcg
     4381 ctccccgcgg cggctcagat actcgttcgc caggacgaag tggttgttcc ccacgaaggg
     4441 gttgcgtgct ccttgagcgc gtggggacgg gtggctggat cggaggatca gatgtttcct
     4501 cgggtcgatc aggtattcct tcttctgagc ctgcag
//