From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Psophocarpus tetragonolobus mRNA for Kunitz chymotrypsin inhibitor
Date: 6 Jan 93 07:01:53 GMT
Distribution: bionet
Checksum: 40286 4

LOCUS       PSTKCIWSP     762 bp ss-mRNA            PLN       06-JAN-1993
DEFINITION  Psophocarpus tetragonolobus mRNA for Kunitz chymotrypsin inhibitor
            with signal peptide.
ACCESSION   D13974
KEYWORDS    kunitz chymotrypsin inhibitor;
            kunitz chymotrypsin inhibitor with signal peptede.
SOURCE      Psophocarpus tetragonolobus (cultivar ishigaki4, strain (L.)DC.)
            Seed, storage deposition stage Embryo cDNA to mRNA, clone pWCI 6-3.
  ORGANISM  Psophocarpus tetragonolobus
            Eukaryota; Plantae; Embryobionta; Magnoliophyta; Magnoliopsida;
            Rosidae; Fabales; Fabaceae.
REFERENCE   1  (sites)
  AUTHORS   Peyachoknagul,S., Matsui,T., Shibata,H., Hara,S., Ikenaka,T.,
            Okada,Y. and Ohno,T.
  TITLE     Sequence and expression of the mRNA encoding the chymotrypsin
            inhibitor in winged bean (Psophocarpus tetragonolobus (L.) DC.)
  JOURNAL   Plant Mol. Biol. 12, 51-58 (1989)
  STANDARD  full automatic
REFERENCE   2  (bases 1 to 762)
  AUTHORS   Habu,Y., Peyachokunagul,S., Umemoto,K., Sakata,Y. and Ohno,T.
  TITLE     Structure and regulated expression of Kunitz chymotrypsin inhibitor
            genes in winged bean (Psophocarpus tetragonolobus (L.) DC.)
  JOURNAL   J. Biochem. 111, 249-258 (1992)
  STANDARD  full automatic
REFERENCE   3  (bases 1 to 762)
  AUTHORS   Habu,Y., Peyachokunagul,S., Umemoto,K., Sakata,Y. and Ohno,T.
  TITLE     Structure and regulated expression of Kunitz chymotrypsin inhibitor
            genes in winged bean (Psophocarpus tetragonolobus (L.)
  JOURNAL   J. Biochem. 111, 249-258 (1992)
  STANDARD  full automatic
COMMENT     Submitted (24-DEC-1992) to DDBJ by: Yoshiki Habu Department of
            Applied Bioscience Faculty of Agriculture Hokkaido University Nishi
            9, Kita 9 Kita-ku, Sapporo Hokkaido 060 Japan Phone:  011-716-2111
            x3887 Email:  yoshiki_habu%al%hines@xmail-gw.sys.hokudai.ac.jp Fax:
               011-746-0208.
FEATURES             Location/Qualifiers
     5'UTR           1..26
                     /partial
     sig_peptide     27..98
                     /codon_start=1
     mat_peptide     99..147
                     /product="Kunitz chymotrypsin inhibitor"
                     /codon_start=1
     3'UTR           651..762
     polyA_signal    708..713
     polyA_signal    728..733
     polyA_site      762
     CDS             27..650
                     /product="Kunitz chymotrypsin inhibitor with signal
                     peptide"
                     /codon_start=1
                     /translation="MKSTTFLALFLLSAIISHLPSSTADDDLVDAEGNLVENGGTYYL
                     LPHIWAHGGGIETAKTGNEPCPLTVVRSPNEVSKGEPIRISSQFLSLFIPRGSLVALG
                     FANPPSCAASPWWTVVDSPQGPAVKLSQQKLPEKDILVFKFEKVSHSNIHVYKLLYCQ
                     HDEEDVKCDQYIGIHRDRNGNRRLVVTEENPLELVLLKAKSETASSH"
BASE COUNT      222 a    168 c    150 g    222 t
ORIGIN      
        1 ggaaatcgtt ccacaagcat aaaaaaatga agagtactac atttcttgcc ctctttctac
       61 tctctgccat catctcacac ctaccatcat ccactgctga tgatgatttg gtcgatgctg
      121 aaggtaactt agttgaaaat ggtggcacat actatctgtt gccacatata tgggcacacg
      181 ggggaggaat agaaacagca aaaacaggaa acgaaccatg ccctctaaca gtggtacgat
      241 ctcccaatga ggtctctaaa ggggaaccaa taaggatctc atcccaattc ctttcattgt
      301 tcatccccag aggctctcta gtggctcttg gattcgctaa ccctccatct tgtgcagctt
      361 ctccgtggtg gactgttgtt gactctccac aaggacccgc tgttaaactt agtcagcaaa
      421 aacttccgga aaaggatatt ctagtgttta aattcgagaa agtttcccat tctaacattc
      481 acgtgtacaa gcttttgtac tgtcaacatg acgaagagga tgtgaagtgt gatcagtata
      541 tcgggattca tagggatcgc aatggaaaca gacgtttggt ggtgactgag gaaaacccat
      601 tagagcttgt gcttctgaaa gctaagtcag aaactgcatc aagccattaa gcacttctca
      661 tccttaaaag tcatctgtgc ttatgactaa atgaatgctt tgcttgtaat aaattgtcac
      721 accactcaat aaaattatct aagtttttgc ttttcttttt tt
//