From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human nonspecific crossreacting antigen mRNA, complete cds.
Date: 5 Nov 92 23:11:25 GMT
Distribution: bionet
Checksum: 42175 6

LOCUS       HUMNCA       2533 bp ss-mRNA            PRI       30-SEP-1988
DEFINITION  Human nonspecific crossreacting antigen mRNA, complete cds.
ACCESSION   M18728
KEYWORDS    nonspecific cross-reacting antigen.
SOURCE      Human lung carcinoma cell line HLC-1, cDNA to mRNA, clones
            lambda-NCA11 and lambda-NCA15.
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 2533)
  AUTHORS   Tawaragi,Y., Oikawa,S., Matsuoka,Y., Kosaki,G. and Nakazato,H.
  TITLE     Primary structure of nonspecific crossreacting antigen (NCA), a
            member of carcinoembryonic antigen (CEA) gene family, deduced from
            cDNA sequence
  JOURNAL   Biochem. Biophys. Res. Commun. 150, 89-96 (1988)
  STANDARD  full automatic
COMMENT     Draft entry and printed copy of sequence for [1] kindly provided by
            Y.Tawaragi, 19-MAR-1988.
FEATURES             Location/Qualifiers
     mRNA            <1..2533
                     /gene="NCA"
     sig_peptide     51..152
                     /gene="NCA"
                     /codon_start=1
     mat_peptide     153..1082
                     /product="non-specific cross reacting antigen"
                     /gene="NCA"
                     /codon_start=1
     CDS             51..1085
                     /product="non-specific cross reacting antigen"
                     /codon_start=1
                     /translation="MGPPSAPPCRLHVPWKEVLLTASLLTFWNPPTTAKLTIESTPFN
                     VAEGKEVLLLAHNLPQNRIGYSWYKGERVDGNSLIVGYVIGTQQATPGPAYSGRETIY
                     PNASLLIQNVTQNDTGFYTLQVIKSDLVNEEATGQFHVYPELPKPSISSNNSNPVEDK
                     DAVAFTCEPEVQNTTYLWWVNGQSLPVSPRLQLSNGNMTLTLLSVKRNDAGSYECEIQ
                     NPASANRSDPVTLNVLYGPDVPTISPSKANYRPGENLNLSCHAASNPPAQYSWFINGT
                     FQQSTQELFIPNITVNNSGSYMCQAHNSATGLNRTTVTMITVSGSAPVLSAVATVGIT
                     IGVLARVALI"
     CDS             1355..1657
                     /product="ORF1"
                     /codon_start=1
                     /translation="MDSFSQDVKTRLLIMIRLLPPFNLSLLMPASFAWQDDAVISISQ
                     EVASEGNLTECQIYLVNPNVLHKIRDPLVHPVTDISSIFNTAVCSNVQWSFSELDF"
     CDS             2370..2501
                     /product="ORF2"
                     /codon_start=1
                     /translation="MLTNVFISVVLFPCSNLTKPTVLVLYCPGGAITVLVEWCCFNS"
BASE COUNT      732 a    648 c    507 g    646 t
ORIGIN      Unreported.
        1 ggagctcaag ctcctctaca aagaggtgga cagagaagac agcagagacc atgggacccc
       61 cctcagcccc tccctgcaga ttgcatgtcc cctggaagga ggtcctgctc acagcctcac
      121 ttctaacctt ctggaaccca cccaccactg ccaagctcac tattgaatcc acgccattca
      181 atgtcgcaga ggggaaggag gttcttctac tcgcccacaa cctgccccag aatcgtattg
      241 gttacagctg gtacaaaggc gaaagagtgg atggcaacag tctaattgta ggatatgtaa
      301 taggaactca acaagctacc ccagggcccg catacagtgg tcgagagaca atatacccca
      361 atgcatccct gctgatccag aacgtcaccc agaatgacac aggattctat accctacaag
      421 tcataaagtc agatcttgtg aatgaagaag caaccggaca gttccatgta tacccggagc
      481 tgcccaagcc ctccatctcc agcaacaact ccaaccccgt ggaggacaag gatgctgtgg
      541 ccttcacctg tgaacctgag gttcagaaca caacctacct gtggtgggta aatggtcaga
      601 gcctcccggt cagtcccagg ctgcagctgt ccaatggcaa catgaccctc actctactca
      661 gcgtcaaaag gaacgatgca ggatcctatg aatgtgaaat acagaaccca gcgagtgcca
      721 accgcagtga cccagtcacc ctgaatgtcc tctatggccc agatgtcccc accatttccc
      781 cctcaaaggc caattaccgt ccaggggaaa atctgaacct ctcctgccac gcagcctcta
      841 acccacctgc acagtactct tggtttatca atgggacgtt ccagcaatcc acacaagagc
      901 tctttatccc caacatcact gtgaataata gcggatccta tatgtgccaa gcccataact
      961 cagccactgg cctcaatagg accacagtca cgatgatcac agtctctgga agtgctcctg
     1021 tcctctcagc tgtggccacc gtcggcatca cgattggagt gctggccagg gtggctctga
     1081 tatagcagcc ctggtgtatt ttcgatattt caggaagact ggcagattgg accagaccct
     1141 gaattcttct agctcctcca atcccatttt atcccatgga accactaaaa acaaggtctg
     1201 ctctgctcct gaagccctat atgctggaga tggacaactc aatgaaaatt taaagggaaa
     1261 accctcaggc ctgaggtgtg tgccactcag agacttcacc taactagaga cagtcaaact
     1321 gcaaaccatg gtgagaaatt gacgacttca cactatggac agcttttccc aagatgtcaa
     1381 aacaagactc ctcatcatga taaggctctt accccctttt aatttgtcct tgcttatgcc
     1441 tgcctctttc gcttggcagg atgatgctgt cattagtatt tcacaagaag tagcttcaga
     1501 gggtaactta acagagtgtc agatctatct tgtcaatccc aacgttttac ataaaataag
     1561 agatccttta gtgcacccag tgactgacat tagcagcatc tttaacacag ccgtgtgttc
     1621 aaatgtacag tggtcctttt cagagttgga cttctagact cacctgttct cactccctgt
     1681 tttaattcaa cccagccatg caatgccaaa taatagaatt gctccctacc agctgaacag
     1741 ggaggagtct gtgcagtttc tgacacttgt tgttgaacat ggctaaatac aatgggtatc
     1801 gctgagacta agttgtagaa attaacaaat gtgctgcttg gttaaaatgg ctacactcat
     1861 ctgactcatt ctttattcta ttttagttgg tttgtatctt gcctaaggtg cgtagtccaa
     1921 ctcttggtat taccctccta atagtcatac tagtagtcat actccctggt gtagtgtatt
     1981 ctctaaaagc tttaaatgtc tgcatgcagc cagccatcaa atagtgaatg gtctctcttt
     2041 ggctggaatt acaaaactca gagaaatgtg tcatcaggag aacatcataa cccatgaagg
     2101 ataaaagccc caaatggtgg taactgataa tagcactaat gctttaagat ttggtcacac
     2161 tctcacctag gtgagcgcat tgagccagtg gtgctaaatg ctacatactc caactgaaat
     2221 gttaaggaag aagatagatc caattaaaaa aaattaaaac caatttaaaa aaaaaaaaga
     2281 acacaggaga ttccagtcta cttgagttag cataatacag aagtcccctc tactttaact
     2341 tttacaaaaa agtaacctga actaatctga tgttaaccaa tgtatttatt tctgtggttc
     2401 tgtttccttg ttccaatttg acaaaaccca ctgttcttgt attgtattgc ccagggggag
     2461 ctatcactgt acttgtagag tggtgctgct ttaattcata aatcacaaat aaaagccaat
     2521 tagctctata act
//