From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: E.virginiana plastid rps14, rps2 and tRNA genes for ribosomal
Date: 15 Aug 92 10:02:50 GMT
Distribution: bionet
Checksum: 15563     9

LOCUS       ORGCPEVRPS   3900 bp ds-DNA             ORG       15-AUG-1992
DEFINITION  E.virginiana plastid rps14, rps2 and tRNA genes for ribosomal
            proteins S14/S2 and tRNA-Ser, tRNA-fMet, tRNA-Glu, tRNA-Tyr,
            tRNA-Asp, tRNA-Asn, tRNA-Cys and tRNA-Arg
ACCESSION   X61798
KEYWORDS    pseudogene; ribosomal protein S14; ribosomal protein S2;
            rps14 gene; rps2 gene; transfer RNA-Arg; transfer RNA-Asn;
            transfer RNA-Asp; transfer RNA-Cys; transfer RNA-Glu;
            transfer RNA-Ser; transfer RNA-fMet.
SOURCE      Chloroplast Epifagus virginiana DNA.
  ORGANISM  Chloroplast Epifagus virginiana
            Eukaryota; Plantae; Embryobionta; Magnoliophyta; Magnoliopsida;
            Asteridae.
REFERENCE   1  (bases 1 to 3900)
  AUTHORS   Morden,C.W., Wolfe,K.H., dePamphilis,C.W. and Palmer,J.D.
  TITLE     Plastid translation and transcription genes in a
            non-sphotosynthetic plant: Intact, missing, and pseudo genes
  JOURNAL   EMBO J. 10, 3281-3288 (1991)
  STANDARD  full automatic
COMMENT     *source: clone=pEV100, pEV802;
            From EMBL    entry CHEVRPS;  dated 10-OCT-1991.
FEATURES             Location/Qualifiers
     CDS             127..429
                     /product="ribosomal protein S14"
                     /gene="rps14"
                     /codon_start=1
                     /translation="MARKSLIQREKGRLKLENKYHFIRRSSKNEISKVPSLSDKWEIY
                     GKLESPPRNSAPTRLRRRCFYTGRPRANYRDFGLCGHILREMVNACLLPGATRSSW"
     tRNA            563..636
                     /product="transfer RNA-fMet"
                     /gene="trnfM(CAU)"
     tRNA            821..913
                     /product="transfer RNA-Ser"
                     /gene="trnS(UGA)"
     tRNA            1072..1144
                     /product="transfer RNA-Glu"
                     /gene="trnE(UUC)"
     tRNA            1225..1309
                     /product="transfer RNA-Tyr"
                     /gene="trnY(GUA)"
     tRNA            1459..1532
                     /product="transfer RNA-Asp"
                     /gene="trnD(GUC)"
     tRNA            complement(1937..2005)
                     /pseudo
                     /gene="trnC(GCA)"
     CDS             2128..2841
                     /product="ribosomal protein S2"
                     /gene="rps2"
                     /codon_start=1
                     /translation="MTRRYWNIDLEEMIGAGVSFGHGTRKWNPKMAPYISVKHKGIHF
                     TNLTKTARFLSEACDLVFYAASRGKQFLIVDTKNKAADSAAWAAIKAWCHCVNKKWPG
                     GMLTNWSTTETRLHKLRDLIMEQKAGRLNRLKKKDEAAVKRQLARLQTYLGGIKYMTR
                     LPDIVIIVDQHEEYKALHECITIGIPTIGLIDTNCDPDLADISIPANDDAISSIRLIL
                     NKLVFAICEGRSGYIIKNI"
     CDS             2909..3110
                     /pseudo
                     /gene="atpA1"
                     /codon_start=1
     CDS             3132..3171
                     /pseudo
                     /gene="atpA2"
                     /codon_start=1
     tRNA            complement(3155..3188)
                     /anticodon=(pos:unspecified,aa:Arg)
                     /pseudo
                     /gene="trnR(UCU)"
     tRNA            3279..3366
                     /product="transfer RNA-Cys"
                     /gene="trnS(GCU)"
     tRNA            3640..3711
                     /product="transfer RNA-Arg"
                     /gene="trnQ(UUG)"
BASE COUNT     1356 a    567 c    686 g   1291 t
ORIGIN
        1 atcgataatt aattatggcc aacctagttg taagatatga tttatatctt acaaaaaaat
       61 ttatttttat attttattta tacatctagg attcgacttg taaaaattaa taggacctga
      121 accattatgg caagaaaaag tttgattcag agggaaaagg gcaggctaaa gttggaaaat
      181 aaatatcatt ttattcgtcg atcctcaaaa aatgaaataa gtaaagttcc atcattgagt
      241 gacaaatggg aaatttatgg aaagttagaa tccccaccac gtaatagtgc acctacgcgc
      301 cttcgtcgtc gttgttttta taccggaagg ccaagagcta actaccgaga ctttggacta
      361 tgcggacaca tacttcgcga aatggttaat gcatgtttgt tgccaggagc aacaagatca
      421 agttggtagg taaggattaa cctttaaatt ttatttggtc gatgataata cctctttatc
      481 tttaccattc tgtataaatg tactatattg tacagatatg gtaaaggcga acatttaatc
      541 cctgttcatt tttattaatt tgggcggggt agagcagttt ggtagctcgc aaggctcata
      601 accttgaggt cacgggttca aatcctgtct ccgcaacatt ttatttttgt ataaaaataa
      661 atagtaagtt tttctctttt aatgttaact agtaattaat taccaccact ctaaaaaaag
      721 gagtcgttag taaaggaaaa atggagggga taaacttatt acactatata tacccaaaat
      781 tttatattag cttaaaatta gcttaaaaaa aattgttttt ggagagatgg ctgagtggtt
      841 gatagccccg gccttgaaaa ccggtatagt ttttaataaa aaactatcgg gggttcgaac
      901 ccctctctct cctttgcccg gatttatata ataataataa attaaaaaat aaagaaaggt
      961 taagagctca gctataccac ctagccaagt cataaaaata aattaaacaa ttatataaat
     1021 aagtaaaaaa aaaaaggcat atctatagta tgaggacagt tgcacaagtt agcccccatc
     1081 gtctagtggt ttaggacatc tctttttcaa ggaggcagcg gggattcgaa ttcccctggg
     1141 ggtaggtaag tagagtacta cggaagttga tagtggatta ctaataagct taaaatatat
     1201 ttgccttttt gggtttgtga ggtggggtcg atgcccgagc ggttaatggg gacggactgt
     1261 aaattcgttg tgtaatatgt ctacgctggt tcaaatccag ctcggcccaa caatacgcca
     1321 acttaaatac cattagtata aagtataaat agcataaatt ctctgtcctt aataaatatc
     1381 ccttatctta taaagaataa agataataaa taatctaatt tttgtaatat ccgttatttt
     1441 aaaattttta aattaccggg gattgtagtc caattggtca gagcaccgcc ctgtcaaggt
     1501 ggaaactgcg ggttcgagcc ccaccagtcc cgacggatcc aataataatt cgttttttat
     1561 ttattatatg aactagtaag gggtatctac aataaaaaaa tatgtaatta cgggtcttaa
     1621 tttatattaa ttaaaaaatt aaaatgaact aataggatta taattataat atagtaatat
     1681 aaagacatag atgtatcttc tggctatctt atctgtctat aaagttgtaa tatagaatta
     1741 agaactttta ttttattttt aataaaaatc taaaatttta tcttcatata tgtgtatatt
     1801 aattatatat attgtatgga atttacataa caccaaaatt gctgcatata tttggtgcga
     1861 taaatattaa ataattttta ttttatgaat ttactaaata aagaagagga aactttactt
     1921 tttttttatg ttgatcgagt gacacccgga ttgagacgga gaaaaaatat ttaaagcccc
     1981 caccttatca ctttgctata aaaaaaaatg aataatctat tcgtttatct atgaccaatc
     2041 cgtggtagaa taaaaggttc cataggaaca attttttaat tcatgattat tcattttttt
     2101 tttatttttt aatggggttg ggtaaaaatg acaagaagat attggaatat agacttggaa
     2161 gagatgatag gggcaggagt ttcttttggg catggtacta gaaaatggaa tccaaaaatg
     2221 gcaccttata tctctgtaaa gcataaaggt attcatttta caaatcttac gaaaactgct
     2281 cgttttttat ctgaagcctg tgatttggtt ttttatgcag caagtagggg aaaacaattc
     2341 ttgattgttg atacaaaaaa taaagcagct gattcagcag catgggctgc aataaaggcc
     2401 cggtgtcatt gtgttaataa aaaatggccc ggagggatgt taacgaattg gtccactaca
     2461 gaaacgcgac ttcataagtt aagggacttg ataatggaac aaaaagcagg aagactcaac
     2521 cgccttaaaa aaaaggatga ggctgccgtg aaaagacaat tagctcgatt gcaaacatat
     2581 ctaggtggta taaaatatat gacaaggtta ccagacatcg taatcatcgt tgatcagcac
     2641 gaagaatata aggctctgca cgagtgtatc accataggaa ttcctacaat tggtttaatc
     2701 gatacaaatt gtgatcccga tctagcagat atttctattc cagcgaatga tgacgctatt
     2761 tcttcaatac gtttaattct taacaaattg gtattcgcta tttgtgaggg ccgttccggc
     2821 tatataataa aaaatatttg atcttgatga agaataatat aaaagttttt tataataccc
     2881 tcctacacgc gtctccggta cctgaaaatc ttatattatg aagaccaccc ggccgtgaag
     2941 cttatattat tcaggggatg tttttatttg tattaacgta tttagtaaag agccactaaa
     3001 ttaataaata aattaagttc tagtttagtt gaagggaccg ccttactaac ttaacttacc
     3061 aaatttaaac ttaatcggga gatgtttcag cttatactac tactaatata taatataaaa
     3121 tttttaaaat agtaagtaaa attgttgttg aattacatac ttacttaaaa aaaatatgta
     3181 aataaattgc gtcctatagg atttgaactt atacctaagg tttagaaaac ctctatccta
     3241 ttttacaacg ggcgcagttc ttttaaatta gagtagtgga aaagatggct gagtggacta
     3301 aagcgtcggg ttgctaatcc gttgtacgag ttattcgtac cgggggttcg aatccctctc
     3361 ttttcgtttc cgtcgatgac ttgctttatt tttttagtaa aaagtaataa agtaggtaag
     3421 gtacaaaaaa atatttattt ctttttattt gaacaacccg cctaattatc aaataaagga
     3481 taatataata aataaaactt tatatacaat atattattaa ttataaatta tatgtaatga
     3541 tcaatattta ttatttttaa aaaccccagt accaatctat agatctttgg gttaaagttg
     3601 acaaaaaact aaataccagt tgtttattgg catttaaaat ggggcgtggc taagtggtaa
     3661 ggcaacgggc tttggtcccg ctattcgtag gttcgaatcc ttccgtccca gtgagtgata
     3721 tacctttttt tatactccgt catataaggt agttaattat aaattttata tctgttttaa
     3781 tatgaaaaat ataaaaacat atgatagatc aaatgttttt ttttatgtgt atattttagg
     3841 cttggctctt aaaaaaaaaa aaaatggata aaataaaaat atatttaaga tatttacagc
//