From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: K.pneumoniae genes sorC, sorD, sorF, sorB, sorA, sorM and sorE
Date: 21 Jul 92 10:06:44 GMT
Distribution: bionet
Checksum: 06172    14

LOCUS       KPNSOR       6853 bp ds-DNA             BCT       21-JUL-1992
DEFINITION  K.pneumoniae genes sorC, sorD, sorF, sorB, sorA, sorM and sorE
ACCESSION   X66059
KEYWORDS    D-glucitol-6-P-dehydrogenase; L-sorbose degradation;
            L-sorbose phosphotransferase system; L-sorbose-1-P-reductase;
            sor operon; sorA gene; sorB gene; sorC gene; sorD gene; sorE gene;
            sorF gene; sorM gene.
SOURCE      Klebsiella pneumoniae DNA.
  ORGANISM  Klebsiella pneumoniae
            Prokaryotae; Gram-negative facultatively anaerobic rods;
            Enterobacteriaceae.
REFERENCE   1  (bases 1 to 6853)
  AUTHORS   Wehmeier,U.F., Woehrl,B.M. and Lengeler,J.W.
  TITLE     The phosphoenolpyruvate-dependent L-sorbose phosphotransferase
            system and its multidomain structure
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
COMMENT     *source: strain=ssp. 1033 5P1, KAY2026;
            From EMBL    entry KPSOR;  dated 10-JUN-1992.
FEATURES             Location/Qualifiers
     misc_binding    784..805
                     /bound_moiety="cAMP/CAP"
     -10_signal      860..865
     RBS             911..916
     CDS             922..1869
                     /product="Sor regulator"
                     /gene="sorC"
                     /codon_start=1
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELGIYRTTISRLLKR
                     GREQGIVTIAINYDYNENLWLEQQLKQKFGLKEAVVASSDGLLEEEQLSAMGQHGALL
                     VDRLLEPGDIIGFSWGRAVRSLVENLPQRSQSRQVICVPIIGGPSGKLESRYHVNTLT
                     YGAAARLKAESHLADFPALLDNPLIRNGIMQSQHFKTISSYWDSLDVALVGIGSPAIR
                     DGANWHAFYGSEESDDLNARHVAGDICSRFYDINGGLVDTNMSEKTLSIEMAKLRQAR
                     YSIGIAMGEEKYSGILGALHGRYINCLVTNRETAELLLK"
     RBS             1928..1933
     CDS             1939..2742
                     /product="D-glucitol-6-P-Dehydrogenase"
                     /gene="sorD"
                     /codon_start=1
                     /translation="MNTWLNLKDNVIIVTGGASGIGLAIVDELLSQGAHVQMIDIHGG
                     DRHHNGDNYHFWSTDISSATEVQQTIDAIIQRWSRIDGLVNNAGVNFPRLLVDEKAPA
                     GRYELNEAAFEKMVNINQKGVFFMSQAVARQMVKQRAGVIVNVSSESGLEGSEGQSCY
                     AATKAALNSFTRSWSKELGKYGIRVVGVAPGILEKTGLRTPEYEEALAWTRNITVEQL
                     REGYTKNAIPIGRAGKLSEVADFVCYLLSARASYITGVTTNIAGGKTRG"
     RBS             2743..2747
     CDS             2752..3159
                     /product="EIII-F Sor PTS"
                     /gene="sorF"
                     /codon_start=1
                     /translation="MVHAIFCAHGQLAGAMLDSVCMVYGEVNVSAVAFVPGENAADIA
                     INLEKLVSAHTDEEWVIAVDLQCGSPWNAAAGLAMRHPQIRVISGLSLPLALELVDNQ
                     HTLSADDLCQHLQAIASQCCVVWQQPETVEEEF"
     RBS             3146..3151
     CDS             3159..3653
                     /product="EIII-B Sor PTS"
                     /gene="sorB"
                     /codon_start=1
                     /translation="MQITLARIDDRLIHGQVTTVWSKVANAQRIIICNDDVFNDEVRR
                     TLLRQAAPPGMKVNVVSLEKAVAVYHNPQYQDETVFYLFTNPHDVLTMVRQGVQIATL
                     NIGGMAWRPGKKQLTKAVSLDPQDIQAFRELDKLGVKLDLRVVASDPSVNILDKINET
                     AFCE"
     RBS             3708..3713
     CDS             3720..4520
                     /product="first subunit of EII-Sor"
                     /gene="sorA"
                     /codon_start=1
                     /translation="MEISTLQIIAIFIFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGVMLGGTLELIALGWMNVGAAQSPDSALASIISAILVIVGHQSIAIGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTIDLLHVSALGVQGLRVAIPALVVSL
                     FVSADMVSSMLSAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDFSLLAFGGVGVIIALIYIQLNPQWRKAEPAASTAPSAPALDQLDD"
     RBS             4522..4526
     CDS             4532..5356
                     /product="second subunit of EII-Sor"
                     /gene="sorM"
                     /codon_start=1
                     /translation="MEQKKITQGDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKADQVAALKRHLVFFNTTPAVCGPVIAVTAAMEEARANGAAIDDGAINGIKVG
                     LMGPLAGVGDPLVWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQL
                     GFRKGVNIVSDMGGNLLQKLTEGASILGLFVMGVLVTKWTTINVPLVVSQTPGADGAT
                     VTMTVQNILDQLCPGLLALGLTLLMVRLLNKKVNPVWLIFALFGLGIIGNALGFLS"
     RBS             5417..5422
     CDS             5428..6660
                     /product="L-Sorbose-1-P-Reductase"
                     /gene="sorE"
                     /codon_start=1
                     /translation="MQTTTALRLYGKRDLRLETFTLPAMQDDEILARVVTDSLCLSSW
                     KEANQGADHKKVPDDVATRPIIIGHEFCGEILAVGKKWQHKFQPGQRYVIQANLQLPD
                     RPDCPGYSFPWIGGEATHVVIPNEVMAQDCLLTWEGDTWFEGSLVEPLSCVIGAFNAN
                     YHLQEGSYNHVMGIRPQGHTLILGGTGPMGLLAIDYALHGPINPSLLVVTDTNKPKLS
                     YARRHYPSEPQTLIHYLDGHEASRDTLLALSGGHGFDDIFVFVPNEQLITLASSLLAP
                     DGCLNFFAGPQDKQFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVALMQAKKVQTAK
                     VVTHILGLNAAGETTLDLPAVGGGKKLVYTGKAFPLTPLGEIADPELAAIVARHHGIW
                     SQEAEAYLLAHAEDITHD"
BASE COUNT     1507 a   1904 c   1921 g   1521 t
ORIGIN
        1 ctcgcacctg cgacggctct ggcggtttaa cccctgcaca agggtgatgc ggaaaacgaa
       61 cggcgcttct ttctttaacc ttacattttt tggtcacggt cccgagaatc ggtacccccg
      121 cgcccatgcc gcgaatgaac tcatcggtca ccggcttatc gaccgtgacc agatattctt
      181 ttcatggtgc ttgccggcgc gcaggatctt gttcaccaga tcgccatggt tggtgaggaa
      241 gatcagcccc tgagaatctt tatccagacg gccaataggg aagatacgct tgctgtggtt
      301 gacgaaatcg acaatgttgt cgcgctcgcc gtcttccgtg gtgctcacaa tgccgaccgg
      361 cttattcagc gcaatcagca ccagatcatc gcttcacgcg gctcaatcaa ccgtccgtta
      421 actttgacta aatcgccagg ctcacctgat cggcaatggt ggcggcttgc cattgataaa
      481 gagcttgcct tgttcaataa agcggtcgct tcgcgacgcg aggcgatagc gcttcgctgt
      541 gtatttattt aatcgggttg atgagtcggg cagcatagat tctcctgtaa aagcgaaata
      601 taccctacct tgtgggcgag aaaaaagatg tgcctctgct ccggcgcaag gtgtgatcga
      661 ctacgccttt tttcacaaaa gtgtcccgct caacgcctgg caactttgca tgatgaaata
      721 tcacagaatc agcgatttag cgtgcaatgc acaaaggtgc caacaacccg ctattgataa
      781 cgcaaatgtg caagattatt cgccagatta gggtgatgaa ataaaagaaa atcgctttaa
      841 acggtggctc taatagggtt atatcagatc aacagcacaa acccgcgatt tgcacaaatg
      901 tgcaggagcc tgcagatgac catggaaaac agtgacgata tccggttgat tgtcaaaatc
      961 gcccagctct attacgagca ggatatgact caggcgcaaa ttgctcgcga gctggggatc
     1021 tatcgcacca ccatcagtcg cctgctgaag cgcggccgcg agcagggcat tgttaccatc
     1081 gccatcaact atgactacaa tgaaaacctg tggctcgagc aacagctcaa gcaaaaattt
     1141 ggcctgaaag aggcggtggt tgccagcagc gatgggctgc tggaagaaga acagctgagt
     1201 gcgatgggcc agcatggcgc cctgcttgtc gatcggttgc tggagccagg cgatatcatc
     1261 ggtttttcat ggggccgcgc cgtgcgttcg ctggtggaga acctgccgca gcgcagccag
     1321 tcacgccagg tgatctgcgt ccccatcatc ggtggacctt ccggtaaact ggagagccgc
     1381 taccatgtga acaccttaac ctacggcgcg gcagccagac tgaaagcgga atcccacctt
     1441 gccgattttc cagccctgct ggataacccg ctgatccgca acggcatcat gcagtcccag
     1501 cactttaaaa ccatctcatc ctactgggac agcctggatg tggcgctggt gggtattggt
     1561 tcaccggcca ttcgcgacgg cgcaaactgg cacgccttct acggcagcga agagagcgac
     1621 gatctcaacg cccgccacgt cgccggggat atctgttcgc gtttctacga tattaacggc
     1681 gggttagtcg ataccaatat gagcgaaaaa accctgtcga tcgaaatggc gaagctgcgc
     1741 caggctcgct attccatcgg catcgccatg ggggaagaga aatactctgg cattcttggc
     1801 gcattgcacg gacgctatat taattgtctg gtgacaaaca gagaaacggc tgagttatta
     1861 ctgaaataac acacaggata tgatttcacg cagcacccgc tgcggggatc cctttatcta
     1921 aagaaatggg agtgaataat gaatacctgg ttaaatttaa aagataacgt cattatcgtg
     1981 accggcggcg cctcgggaat tgggctggcc attgtcgatg aattattatc acaaggtgct
     2041 catgtccaga tgattgatat tcatggcggc gatcgtcatc acaatggcga taattatcac
     2101 ttctggtcga cggatatttc cagcgcgaca gaggtgcaac agactatcga tgccattatt
     2161 cagcgctggt cgcgtattga tggtttggtc aataacgctg gcgtgaattt cccgcgttta
     2221 ttagtcgacg aaaaagcacc ggccggccgc tatgaattaa acgaagccgc ttttgaaaaa
     2281 atggtcaata tcaaccagaa aggggtgttt ttcatgtcgc aggcggtggc gcgtcaaatg
     2341 gtcaaacagc gcgccggtgt gattgtcaat gtctcttcgg agagcggcct ggaaggctct
     2401 gaaggtcaaa gctgctacgc cgcgaccaag gccgcgctca acagctttac ccgctcctgg
     2461 tccaaagaat tgggtaaata tgggatccgc gtggtcggcg ttgcgccggg gatcctcgaa
     2521 aaaaccggtc tgcggacacc ggaatatgaa gaggcgctgg cctggacgcg caatatcacc
     2581 gtcgagcaac ttcgcgaggg atataccaaa aacgccattc ccatcgggcg ggcaggaaaa
     2641 ctctcagaag tcgctgattt tgtttgctat ctcttgtcag cgcgcgccag ctacatcacc
     2701 ggagtcacca ctaacattgc cggcggaaaa acgcgcggtt aaggaggcag tatggttcac
     2761 gctatcttct gcgcccacgg ccagctggcc ggggccatgc ttgattcggt atgcatggtc
     2821 tacggcgagg ttaacgtcag cgccgtcgcg tttgtccccg gcgaaaacgc ggcggatatc
     2881 gccattaacc tggaaaagtt agtaagcgcc cacaccgatg aggagtgggt aatcgcggta
     2941 gatttgcagt gcggaagccc atggaatgcc gcagccgggc tggcaatgcg tcacccgcag
     3001 atccgggtga ttagcggcct gtcgctgccg ctggcgctcg agctggtgga taaccagcat
     3061 accctgagcg ccgatgactt atgccagcat ctgcaggcca tcgccagtca gtgctgtgtg
     3121 gtctggcagc agccagaaac cgttgaggag gagttctgat gcaaattacc ctcgcccgta
     3181 ttgatgaccg actgattcat ggccaggtca ccaccgtgtg gtcaaaagtc gccaacgccc
     3241 agcggataat tatctgcaat gacgatgtat ttaacgatga ggttcgccgg accctgttgc
     3301 gccaggcggc tccgccaggc atgaaggtaa acgttgtcag tctggaaaaa gcggttgcgg
     3361 tctatcataa cccgcaatat caggacgaga ccgtctttta tttatttacc aatccacacg
     3421 atgttttaac gatggtgcgc caaggcgtgc agatcgccac gttaaatatt ggtggcatgg
     3481 cctggcgacc cggtaaaaaa cagctaacca aagccgtttc tttggatccg caggatattc
     3541 aggcattccg tgaactcgat aaactgggcg taaaactcga tttacgcgtg gtcgcatcag
     3601 atccgtcagt caatattctc gacaaaatta acgaaacagc tttctgcgaa taaaaaatag
     3661 cgcctgttgt gatatgccgt agcaggcagg atgactcaca ccttaaaggt gcataaatta
     3721 tggaaattag taccctacag ataatagcca tatttatttt ttcctgtatt gccggaatgg
     3781 gcagcgtgct ggatgaattt cagacccatc ggccccttat cgcctgtacc gtgattggcc
     3841 tcatcctggg cgatttaaaa accggggtta tgctcggcgg tacgctggag ctgatcgccc
     3901 tcggctggat gaacgtgggg gcagcgcagt cgccagattc ggcgctggcc agcattatct
     3961 ccgccattct ggtgattgtg ggccaccaga gcattgccat tggtatcgcc attgctctgc
     4021 cggtggccgc cgccgggcag gtgctgaccg ttttcgcccg taccattacc gtggtgttcc
     4081 agcacgccgc ggacaaagcg gccgaggagg cgcgctttcg caccatcgac ctgctgcatg
     4141 tctccgcgct gggggtgcag ggcctgcgcg tggcaatccc ggcgctggtg gtctcgctgt
     4201 tcgttagcgc ggatatggtc agcagtatgc tcagcgcgat cccggaattc gtcacccgcg
     4261 gcctgcagat tgccggtggt ttcattgtcg tcgtgggcta cgcgatggtg ctgcgaatga
     4321 tgggcgtgaa atacctgatg cccttctttt tcctcggttt tctcgccggg ggttatctcg
     4381 acttcagcct gctggccttc ggcggcgtgg gcgtcatcat cgcgctgatc tacatccagc
     4441 tcaatccaca gtggcgtaag gctgaacccg ccgcctccac tgccccctct gcccccgccc
     4501 ttgaccagct tgacgactaa tggagccgaa aatggaacag aaaaaaatca cgcaaggcga
     4561 cctggtgagc atgtttctcc gctccaacct ccagcaggcc tcctttaact tcgaacgtat
     4621 tcatggcctg gggttttgct acgacatgat cccggcgatc aaacgcctgt atccgctcaa
     4681 agccgatcag gtcgcggcgc tgaagcgtca tctggtgttc tttaatacca cgccggcggt
     4741 gtgtggcccg gtgatcgccg tcaccgccgc catggaggag gcccgggcta acggcgcggc
     4801 cattgacgat ggcgctatca acggcatcaa agtgggtctg atgggcccgc tggccggcgt
     4861 cggcgacccg ctggtctggg gcacgctgcg gccgattact gcggcccttg gcgcctcgct
     4921 ggccctctcc ggcaacattc tgggaccttt gttatttttc tttattttca atgcagtgcg
     4981 gctggcaatg aagtggtacg gcctgcaact gggcttccgt aaaggggtca atatcgtcag
     5041 cgatatgggc ggtaacctgc tgcagaagct gaccgaaggc gcctccattc tcggcctgtt
     5101 tgtcatgggg gtgctggtga ccaaatggac caccatcaat gtgccgctgg tggtttccca
     5161 aacgcccggt gcagacggcg ccaccgtcac gatgaccgtc cagaacatcc tcgatcagct
     5221 ctgccccggc ctgctggcgc tgggcctgac gctactgatg gtgcgtctgc tgaacaagaa
     5281 agtgaatccg gtctggctga ttttcgccct tttcggctta ggcattatcg gcaacgccct
     5341 cggctttctg tcctgattat tccgcccggc gatgccgggg tgacgtcaac aacatagccc
     5401 cgaaagatga aggggatgag gtggtttatg caaacaacaa cggccctgcg cctgtatggc
     5461 aaacgagacc tgcgcctgga aacctttacc ctcccggcga tgcaggacga tgagatcctc
     5521 gcccgggtgg tcacggacag cctgtgtctt tcctcatgga aagaggccaa tcagggtgcc
     5581 gatcataaaa aggtgccaga tgatgtggcc accaggccca ttattatcgg tcatgaattc
     5641 tgcggcgaaa tccttgccgt cggtaaaaag tggcagcata agtttcagcc agggcagcgc
     5701 tacgtgatcc aggccaacct gcagttgccc gaccgacccg actgccccgg ctactcattc
     5761 ccatggatcg gtggcgaagc cacccatgtg gtgatcccca atgaggtgat ggcgcaggat
     5821 tgcctgctca cctgggaggg ggatacctgg tttgaaggat cgctggtgga gccgctctcc
     5881 tgcgtcattg gcgctttcaa cgccaattat catctgcagg aggggagtta caaccacgtg
     5941 atggggatcc gtccgcaggg acacactctg atcctgggcg ggacggggcc gatggggctg
     6001 ctggctatcg actatgcgct gcacggcccc atcaatcctt cactactggt agtgaccgat
     6061 accaataagc cgaagctcag ctacgcccgc cgtcattacc cctctgagcc gcagacgctg
     6121 atccactacc tcgacggcca tgaggccagt cgcgatacgc tgctggcgct cagcggcggc
     6181 catggcttcg acgatatctt cgtgtttgtg ccaaacgaac agctgatcac cctggcctcc
     6241 tcgttgctgg ctccggacgg ctgcctgaat ttctttgctg gcccccagga taagcaattc
     6301 agcgccccga tcaacttcta cgacgtgcac tacgccttca cccactacgt cggcacctcc
     6361 ggcggtaata ctgacgatat gcgcgccgcg gtggcgctga tgcaggcgaa aaaggtccag
     6421 acggcgaaag tggtcaccca tattctcggc ctgaacgccg cgggcgaaac caccctcgat
     6481 ctgcctgccg tcggcggggg aaaaaagctg gtgtataccg gaaaagcctt cccgctcacg
     6541 ccgctgggcg agatcgccga tcccgaactg gcagcgattg tggcgcgtca ccatgggatc
     6601 tggtcccagg aggctgaagc gtatctgctg gcccacgcgg aggatattac gcatgattaa
     6661 ccgcgatacg ctgctgtgca tctccctggc gggtcgcccc ggcaacttcg gcacccgctt
     6721 tcataactat ctgtatgaca agctgggatt gaactacctc tacaaagcct ttacgactaa
     6781 ggatattgag gcggcggtaa aaggggttcg cgcattgggt atccgcggct gtgcggtctc
     6841 catgccgttt aaa
//