From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Spinach sucrose phosphate synthase (sps1) mRNA, complete cds.
Date: 5 Nov 92 23:13:30 GMT
Distribution: bionet
Checksum: 42215 8

LOCUS       SPISPS1A     3530 bp ss-mRNA            PLN       14-OCT-1992
DEFINITION  Spinach sucrose phosphate synthase (sps1) mRNA, complete cds.
ACCESSION   L04803
KEYWORDS    UDP glucuronosyltransferase; sucrose phosphate synthase.
SOURCE      Spinacia oleracea (strain Melody) leaf cDNA to mRNA.
  ORGANISM  Spinacia oleracea
            Eukaryota; Plantae; Embryobionta; Magnoliophyta; Magnoliopsida;
            Caryophyllidae; Caryophyllales; Chenopodiaceae.
REFERENCE   1  (bases 1 to 3530)
  AUTHORS   Salvucci,M.E. and Klein,R.R.
  TITLE     Identification of uridine binding domain of sucrose-phosphate
            synthase. Cloning and expression of a region of the protein that
            photoaffinity labels with 5-azidouridine diphosphate-glucose
  JOURNAL   Unpublished (1993)
  STANDARD  full automatic
REFERENCE   2  (bases 1 to 3530)
  AUTHORS   Klein,R.R., Crafts-Brandner,S.J. and Salvucci,M.E.
  TITLE     Cloning and developmental expression of sucrose phosphate synthase
            from spinach
  JOURNAL   Unpublished (1993)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     5'UTR           1..86
                     /gene="sps1"
     3'UTR           3255..3530
                     /gene="sps1"
     CDS             87..3257
                     /function="synthesis of sucrose phosphate"
                     /EC_number="2.4.1.14"
                     /gene="sps1"
                     /product="sucrose phosphate synthase"
                     /codon_start=1
                     /translation="MAGNDWINSYLEAILDVGGQGIDASTGKTSTAPPSLLLRERGHF
                     SPSRYFVEEVISGFDETDLHRSWVRAASTRSPQERNTRLENLCWRIWNLARKKKQIEG
                     EEAQRLAKRHVERERGRREATADMSEDLSEGERGDTVADMLFASESTKGRMRRISSVE
                     MMDNWANTFKEKKLYVVLISLHGLIRGENMELGRDSDTGGQVKYVVELARALGSMPGV
                     YRVDLLTRQVSAPGVDWSYGEPTEMLSSRNSENSTEQLGESSGAYIIRIPFGPKDKYV
                     AKELLWPYIPEFVDGALSHIKQMSKVLGEQIGGGLPVWPASVHGHYADAGDSAALLSG
                     ALNVPMVFTGHSLGRDKLDQLLKQGRLSREEVDATYKIMRRIEAEELCLDASEIVITS
                     TRQEIEEQWQLYHGFDLVLERKLRARMRRGVSCHGRFMPRMAKIPPGMEFNHIAPEDA
                     DMDTDIDGHKESNANPDPVIWSEIMRFFSNGRKPMILALARPDPKKNLTTLVKAFGEC
                     RPLRELANLTLIIGNRDDIDEMSTTSSSVLISILKLIDKYDLYGQVAYPKHHKQSDVP
                     DIYRLAAKTKGVFINPAFIEPFGLTLIEAAAYGLPIVATKNGGPVDIIGVLDNGLLID
                     PHDQKSIADALLKLVADKHLWTKCRQNGLKNIHLFSWPEHCKNYLSRIASCKPRQPNW
                     QRIDEGSENSDTDSAGDSLRDIQDISLNLKLSLDAERTEGGNSFDDSLDSEEANAKRK
                     IENAVAKLSKSMDKAQVDVGNLKFPAIRRRKCIFVIALDCDVTSDLLQVIKTVISIVG
                     EQRPTGSIGFILSTSMTLSEVDSLLDSGGLRPADFDAFICNSGSELYYPSTDYSESPF
                     VLDQDYYSHIDYRWGGEGLWKTLVKWAASVNEKKGENAPNIVIADETSSTTHCYAFKV
                     NDFTLAPPAKELRKMMRIQALRCHAIYCQNGTRLNVIPVLASRSQALRYLFMRWGVEL
                     SNFVVFVGESGDTDYEGLLGGVHKTVILKGIGSNTSNFHATRAYPMEHVMPVDSPNMF
                     QTGGCNIDDISDALSKIGCLKAQKSL"
BASE COUNT     1019 a    640 c    845 g   1026 t
ORIGIN      
        1 gacgccacac tatttcttcc atcttcttct cttccgaatt ttttaaaaaa agttcaacaa
       61 atttcacaga tttcgatcgg gaaaaaatgg cgggaaacga ctggattaac agttatctag
      121 aagccatttt agacgtcgga ggtcaaggta tcgacgcttc tactggaaaa acgtcgactg
      181 cgccgccgtc gttgcttctc cgtgaacgag gtcattttag tccttctcgt tattttgttg
      241 aagaagttat ctctggtttt gatgagaccg atcttcatcg ctcttgggtt cgcgctgcat
      301 caactcgcag ccctcaagag aggaatacac ggttggaaaa tctgtgctgg agaatatgga
      361 atttagcacg caaaaagaaa cagattgagg gagaggaagc tcagcgtctg gctaaacgcc
      421 atgtggaacg cgaaagaggt cgcagagagg ctactgctga catgtcagaa gacttgtccg
      481 aaggagaaag gggagacacc gttgctgata tgttatttgc tagtgaaagc actaaaggaa
      541 gaatgcgtag aataagttca gtggaaatga tggacaattg ggccaatacc ttcaaagaaa
      601 agaaactcta cgttgtgttg ataagccttc atggcttgat acgtggtgag aacatggaac
      661 ttggtcgcga ttctgataca ggtggtcagg taaagtatgt agtagaactt gccagggcgc
      721 ttggatcaat gcctggagtt taccgcgttg atctactgac aagacaggtt tcagctccag
      781 gtgtagattg gagttatggt gaacctactg agatgttgtc ttcaagaaat tctgaaaatt
      841 caactgagca gcttggagag agcagcggtg cttacattat ccgcatacca tttgggccga
      901 aggacaaata tgtagcaaaa gagttacttt ggccatacat tccagagttt gttgatggtg
      961 cacttagtca cataaaacaa atgtccaaag ttcttggtga gcaaattggt ggtgggttgc
     1021 ctgtatggcc agcttctgtc catgggcatt acgctgatgc tggggattct gcagctcttt
     1081 tatccggtgc tttaaatgtg ccgatggttt ttactggtca ttctcttgga cgagataagc
     1141 ttgaccagct gctgaaacag gggagactct ctagggaaga agttgatgca acatacaaaa
     1201 taatgcggcg catagaggct gaggagttat gtcttgatgc ctctgaaata gttatcacaa
     1261 gcactagaca ggaaatagaa gagcaatggc agctctatca tggatttgat ctagtgctag
     1321 aacgcaaact aagagctagg atgagacgtg gtgtgagctg tcacggtaga tttatgcccc
     1381 gcatggctaa aattcctcct ggcatggaat ttaaccatat cgctccagaa gatgctgata
     1441 tggacacaga tattgatgga cataaagaaa gcaatgcgaa tccagatcca gttatttggt
     1501 ctgagattat gcgttttttt tcaaatgggc gcaagccaat gatacttgcc cttgctaggc
     1561 cagatccaaa aaagaacctt acaacattag tcaaggcttt tggtgaatgc cggccattga
     1621 gagaactcgc aaatctgact ttgattattg gaaatcgtga tgatattgat gaaatgtcta
     1681 ccacaagttc atctgtcctg atctcgatcc ttaaattgat tgacaagtat gatctgtatg
     1741 gtcaagtggc gtatcctaag caccacaagc agtctgatgt tcctgatatt tatcgcctgg
     1801 cagctaaaac aaagggtgta ttcatcaatc ctgcgtttat tgaaccattt gggctgactt
     1861 taattgaggc tgctgcttat ggtttgccta ttgttgctac aaagaatgga ggccctgttg
     1921 acataattgg ggttctggac aatgggcttc tcattgatcc tcatgatcag aaatctattg
     1981 ctgatgctct cttgaagctt gttgctgata aacacctatg gacgaagtgt agacagaatg
     2041 ggttgaagaa cattcacctc ttttcgtggc cagagcattg caaaaattac ttgtcacgca
     2101 tagccagttg caaacctagg caaccaaatt ggcaaaggat cgatgaagga tctgaaaatt
     2161 cagatacaga ttcagctggt gattctttga gggacattca agatatatct ttgaacttaa
     2221 agctttcact ggatgcagag aggactgaag gaggcaacag ttttgatgat tcattagatt
     2281 ccgaagaagc taatgcaaag agaaaaatag agaatgcagt tgcgaaattg tcaaagtcta
     2341 tggataaagc acaggttgat gttggtaatc taaagttccc agctattagg aggagaaagt
     2401 gtatttttgt catcgctctg gattgtgatg tgacttcaga tctccttcaa gtcattaaga
     2461 cagttatttc tattgtcgga gagcaaagac caacagggtc aattggattt atactctcaa
     2521 catcaatgac cttatctgaa gttgattctc ttttggattc aggaggcttg agacctgcag
     2581 attttgatgc tttcatttgt aacagtggca gtgaacttta ctatccatct acggactatt
     2641 cagagtctcc gtttgtgcta gatcaagact attattcgca tattgactac cgttggggag
     2701 gagaaggttt gtggaagact ttggtgaaat gggctgcttc cgtgaacgag aaaaagggag
     2761 agaatgctcc aaatattgtt attgcagatg aaactagctc tacaactcac tgctatgcat
     2821 ttaaagtgaa tgatttcact ttggcacccc cagctaagga gcttaggaag atgatgagaa
     2881 tccaggctct acgttgtcat gcgatatatt gccaaaatgg taccaggttg aatgtcattc
     2941 ctgttttggc ttcaagatcc caagcactca gatacctatt tatgcgttgg ggagtagagt
     3001 tgtcaaattt cgtggttttt gttggcgaat ctggagatac agattatgaa ggattgttgg
     3061 gtggggttca caagacggtg atattaaagg gcatcggaag taatacttct aattttcatg
     3121 caaccagagc atatcctatg gagcatgtta tgccagttga cagccccaac atgttccaga
     3181 ctggaggatg taacattgac gacataagtg acgccctaag caagattggc tgtttaaaag
     3241 cacagaagag tctttaagac attatagcgt tgctgatttc gagccaccac agtttttttt
     3301 tttaaatata aatcttcatc aattgtcatt actctttata taccttgctg gtcatatttc
     3361 tgcaggaaag tttcgctggg aataggtcag ttgaatgttc tcttcaactt aattgtatat
     3421 cttgttgcat tattttttta gtttagatag ggctggaagc cgaaacataa aggcagtaaa
     3481 aagagactgg aaaagtttct gtaatctgta caatctgaaa aacaattttt
//