From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: S.typhimurium nirC and open reading frames 3 and 4, complete cds
Date: 27 Jan 93 06:02:29 GMT
Distribution: bionet
Checksum: 17119 10

LOCUS       STYCYSAA     5280 bp ds-DNA             BCT       26-JAN-1993
DEFINITION  S.typhimurium nirC and open reading frames 3 and 4, complete cds
            and cysG gene, 3' end.
ACCESSION   M64606
KEYWORDS    cysG gene; nirC gene.
SOURCE      Salmonella typhimurium DNA.
  ORGANISM  Salmonella typhimurium
            Prokaryotae; Gram-negative facultatively anaerobic rods;
            Enterobacteriaceae.
REFERENCE   1  (bases 1 to 5280)
  AUTHORS   Wu,J.-Y., Siegel,L.M. and Kredich,N.M.
  TITLE     High-level expression of escherichia coli nadph-sulfite reductase:
            requirement for a cloned cysG plasmid to overcome limiting siroheme
            cofactor
  JOURNAL   J. Bacteriol. 173, 325-333 (1991)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     CDS             469..1278
                     /gene="nirC"
                     /codon_start=1
                     /translation="MFTDSINKCAAKLRASAPVSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTLGVKAGTISHGQMW
                     AILPQTWLGNLVGSVFVALLYSWGGGSLLPVDTSIVHSVALAKTTAPATVLFFKGALC
                     NWLVCLAIWMAIRTEGTAKFLAIWWCLLAFIASGYEHSVANMTLFALSWFGHHSDAYT
                     LAGIGHNLLWVTLGNTLSGVVFMGLGYWYATPKSERPAPAKINQPEAAANN"
     CDS             1290..2663
                     /gene="cysG"
                     /codon_start=1
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLEAGARLTVNALT
                     FIPQFTVWANEGMLTLVEGPFDETLLDSCWLAIAATDDDTVNQRVSDAAESRRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPQHLGQVARYAGQ
                     LRARVKKQFATMGERRRFWEKFFVNDRLAQSLANADEKAVNATTERLFSEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADIVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCHAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLVTGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQE
                     KLIAFGMQADMPVALVENGTSVKQRVVHGVLTQLGELAQQVESPALIIVGRVVALRDK
                     LNWFSNH"
     CDS             2997..4247
                     /note="putative;  ORF 3"
                     /codon_start=1
                     /translation="MQKKKLISIAIALTLQSYYIPAIAAENNDDEKECPSNISSLPKE
                     KRAKLSPTCLATPENDNHWGWVAGGVAALVAGVAIGVENNGGGDSNHSYTPPKPDNGG
                     DVTPPDDGGNVTPPDDGGNVTPPDDGGDDNVTPPDDSGDDDVAPPDDSGDDDVTPPDD
                     SGDDDVTPPDDSGDGDVTPPDDSGDDDVTPPDDSGDDDVTPPDDSGDDDVTPPDDSGD
                     DDVTPPDDSGDDDVTPPDDSGDDDDTPPDDSVITFSNGVTIDKGKDTLTFDSFKLDNG
                     SVLEGAVWNYSEQDNQWQLTTADGKTLNVTGWDVTDANAAVIEGTQENGLYWKYDSRG
                     YLIIADDNTTVISGDDQAHNSDRGMDISGQDRTGVIISGDRTVNTLTGDSSVTDGATG
                     MVISGDGTTNTISGHSTVDKPPAR"
     CDS             4433..5227
                     /note="ORF 4"
                     /codon_start=1
                     /translation="MTITDGGTGGHITGDNVVIDNAGSTTVSGADRTALYIEGDNALV
                     INEGNQTISGGAVGTRIDGDDAHTTNTGDIAVDGAGSAAVIINGDNGSLTQAGDLLVT
                     DGAMGIITYGTGNEAKNTGNATVRDADSVGFVVAGEKNTFKNKGDIDVSLNGTGALVS
                     GDMSQVTLDGDINVVSVQDSEGVFSSATGVSVSGDSNAVDITGNVNISADYGQDDLAA
                     GAPPLTGVVVGGNGNTVTLNGALNIDDNDLSHRRTIPGRCWPERNR"
BASE COUNT     1226 a   1458 c   1467 g   1129 t
ORIGIN      
        1 gatcagcaac atcgacccgt tctttgaggc cagtgtgctg tctcgtgggc tgattgcgga
       61 acaccagggc gaactgtggg tcgccagccc gttgaaaaag cagccgttcc gcctgagcga
      121 tggtttatgc atggaagatg agcagttttc tgtgaaacat tacgacgcgc gggtaaaaga
      181 cggcgtggtg cagttgcgcg gttaattatt tttggaggcg caacgcctcc ccttttttgt
      241 attttttttc attttaattg ttatcacaaa atcatttgta ctgcatcgcg gcggcaactg
      301 agcaacaaat tcgtcaggaa cgaatttgaa cagccgctgg ctgccttcgg tgagggacaa
      361 ggatgtccct cattaatccc caggagatag ttcactatgt gactggggtg agcgagggca
      421 gccaacaaag aggcagtgca aaggatgaag tgagaaaagg ataatcaaat gtttacagac
      481 tctatcaata agtgtgcggc taagctgcgc gcatctgcac ccgtgtcggc gaataatccg
      541 ctcggcttct gggtcagttc ggcaatggcc ggggcttatg tcggtctcgg catcattctt
      601 attttcaccc tcggcaatct gctcgacccg tccgtacgtc ctctggtgat gggagccacc
      661 ttcggtatcg ccttaacgct ggtcatcatc gccggttccg aactgtttac cggccacacc
      721 atgttcctga cgctgggcgt caaagcaggc accatcagcc acggtcaaat gtgggctatc
      781 ctgccgcaaa cctggctcgg caacctggtc ggttccgtgt ttgtcgccct gctttacagc
      841 tggggcggcg gcagtttatt gccggtcgat accagcatcg ttcactcagt cgcgctggcg
      901 aaaaccaccg cgcccgccac ggtactgttc ttcaaaggcg cgctgtgtaa ctggctggtt
      961 tgtctggcaa tctggatggc aatccgcacc gaaggcacgg caaaatttct tgctatctgg
     1021 tggtgtctgc tggcgtttat cgcttccggc tacgagcact ccgtcgcgaa tatgacgctg
     1081 ttcgccctct cctggtttgg tcatcacagc gacgcctata ccctggccgg aattggtcat
     1141 aacctgttgt gggtgacact cggtaatact ttgtccggtg tcgtattcat gggattgggt
     1201 tattggtatg ctacgccaaa atcggagcgt ccggctccgg caaaaatcaa tcagccagag
     1261 gctgctgcca ataattaagg ggtaatgtcg tggaccattt gcctatattt tgtcaattac
     1321 gcgaccgcga ctgtctgatc gtcggcggtg gcgatgtcgc agaacgcaaa gcacggttac
     1381 tgctggaagc aggcgcacgt ttaacggtca atgcgctaac ctttattcca cagttcaccg
     1441 tatgggcaaa tgaaggcatg ttgactctgg ttgagggacc gttcgacgaa acgcttctcg
     1501 actcgtgttg gctggcgatc gcggccactg acgacgatac cgtcaaccag cgcgtcagcg
     1561 acgcggcgga gtcacgccgt atcttttgca acgtggtgga tgcgccgaaa gccgccagct
     1621 ttatcatgcc ctccattatt gaccgctcgc cgctgatggt cgccgtctcc tcgggcggca
     1681 cctccccggt gctggcgcgt ctgctgcgcg agaaactgga atcgctgctg ccgcagcatc
     1741 tggggcaggt cgcgcgctat gccgggcaac tccgcgcccg agtgaaaaag cagtttgcca
     1801 cgatgggcga gcgtcgtcgc ttctgggaaa aatttttcgt caatgaccgg ctggcgcagt
     1861 cgctggcgaa tgccgatgag aaagcggtta acgcgacaac cgaacgcctg tttagcgaac
     1921 cgctggatca ccgtggcgaa gtcgtgctgg tcggcgccgg gccgggcgat gccggactgc
     1981 tgacgctgaa agggttacaa caaatccaac aggcggatat cgtggtttac gatcgcctcg
     2041 tctccgacga cattatgaac ctggtacgcc gcgatgccga tcgggtcttt gtggggaaac
     2101 gcgcgggtta ccactgcgtc ccacaggagg aaatcaacca gatcctgctg cgtgaagcgc
     2161 aaaaaggtaa acgcgtggta cgcctgaaag gcggcgatcc ctttatcttt ggtcgcggcg
     2221 gcgaagagct ggaaacgctg tgtcatgccg gtattccttt ctcggtagtg ccggggatta
     2281 ccgcggcttc cggctgctcc gcctactccg gtattccgct aactcatcgc gattacgccc
     2341 agagcgtacg tctggtcacg ggtcacctga aaaccggcgg cgagctggac tgggaaaacc
     2401 tggcggcaga aaaacagacg ctggtgttct acatggggct gaatcaggca gcgactatcc
     2461 aggaaaaact gatcgcattc ggtatgcagg ccgatatgcc ggttgcgctg gtagaaaacg
     2521 gtacctccgt gaagcaacgc gtcgtccacg gtgtgctgac gcagctcggt gaattagcgc
     2581 aacaggttga aagcccggcg ctgattatcg ttggtcgcgt ggtagcctta cgcgataaat
     2641 taaattggtt ctctaatcat taattaaaat aacgccctgt tatcagggct ttattttaca
     2701 actactcgta atctcaaatt atttttactt aaaagtgaat taagaaacta actttaaata
     2761 caccggacaa atttaaataa taattctgcc taaaaaccct tttactcgtc aaattcacct
     2821 cttattcatt catacaataa ataacaccgt taagcactca atttgacctg acctggttat
     2881 cgggtgataa aataaacact aaagcataat ttttcttctg gccatttcat cattgcctgt
     2941 accgctctct gcatatgttt agtacgcaag gaaaatatta attaaggatg aaccctatgc
     3001 aaaagaaaaa acttatttct atcgctatcg ctttaacgct acaaagttat tacattccgg
     3061 ccatcgccgc agaaaataac gatgatgaaa aagaatgtcc cagtaatatc tcctccctgc
     3121 ctaaagaaaa acgcgcaaaa ctctcaccga cctgccttgc tacacctgaa aatgataatc
     3181 actggggctg ggttgctggc ggcgttgctg cattggtcgc aggtgtggcg attggcgttg
     3241 aaaataacgg tggcggagat tctaatcatt cttatacccc ccctaagccc gataatggcg
     3301 gcgacgtcac cccgcccgac gatggcggca acgtcacccc gcccgacgat ggcggcaacg
     3361 tcaccccgcc cgacgatggc ggcgatgaca atgtgacccc gcccgacgat agtggcgatg
     3421 acgatgtggc cccgcctgac gatagcggcg atgacgatgt aaccccgccc gacgatagcg
     3481 gcgatgatga tgtgaccccg cccgacgata gcggcgatgg cgatgtgacc ccgcccgacg
     3541 atagcggcga tgacgatgta accccgcccg acgatagcgg cgatgacgat gtgaccccgc
     3601 ctgacgatag cggcgatgac gatgtaaccc cgcccgacga tagcggcgat gacgatgtga
     3661 ccccgcccga tgatagcggc gatgacgatg taaccccgcc cgatgatagc ggcgatgacg
     3721 acgacacgcc cccagatgac tctgttatta ccttcagcaa cggcgtcacc atcgataaag
     3781 gcaaagacac cctgaccttc gacagcttca aactggataa cggcagcgtt cttgagggtg
     3841 ccgtgtggaa ttattcagaa caggacaacc agtggcagct caccaccgcg gacggtaaaa
     3901 cgctgaacgt caccggctgg gacgtgaccg acgccaatgc cgccgtgatt gaaggcaccc
     3961 aggaaaacgg tctctactgg aagtacgaca gccggggcta tctgattatt gccgacgata
     4021 acaccaccgt tatcagcggc gatgaccagg cgcataattc cgatcgcggc atggatatca
     4081 gcggccagga tcgcaccggc gtgattattt ccggcgatag aaccgtcaac acgctcaccg
     4141 gggactccag tgtgaccgac ggtgccaccg gcatggttat ctccggtgac ggcaccacca
     4201 acaccatttc gggccactcc acggtggaca agccaccggc gcgctgattt ccggcaacgg
     4261 caccaccacc aatttcgccg gtgacattgc cgtgagcggc ggcggcaccg ccatcatcat
     4321 cgacggcgac aacgccacga ttaagaatac cggtacctct gacatcagcg gcgcaggctc
     4381 caccggcacc gtcattgacg gcaataacgc ccgcgtcaac aatgacggtg atatgaccat
     4441 caccgacggc ggcaccggcg gccacattac cggcgacaac gtggttatcg ataacgccgg
     4501 gagcactacc gtcagcggcg cagaccgcac ggcgctgtat atcgaaggcg acaacgcgct
     4561 cgttatcaac gaaggtaatc aaactatctc tggcggcgcc gtcggtacgc gcattgacgg
     4621 cgacgacgcc cataccacca ataccggtga tatcgcggtg gatggcgcgg gctctgccgc
     4681 cgtgattatc aacggcgaca acggcagcct gacccaggcg ggcgatctgc tggtcaccga
     4741 cggcgcgatg ggcatcatca cctatggcac cggaaatgaa gcaaaaaata ccggcaacgc
     4801 caccgtacgt gatgcggact cggtgggttt tgtggttgca ggcgaaaaaa acaccttcaa
     4861 aaacaaaggg gatattgacg tcagccttaa cggcaccggc gcgctggtga gcggcgatat
     4921 gtcgcaggtt acgctggatg gcgatattaa cgttgtctca gtccaggaca gcgaaggcgt
     4981 gtttagctca gcgacagggg tgagcgtgag cggcgacagc aacgccgttg atatcaccgg
     5041 caacgtaaat atcagcgccg actacgggca ggatgatctg gctgccgggg ctcccccgtt
     5101 aaccggcgtt gtcgtcggcg gtaacggcaa taccgttacc cttaatggcg cgctgaatat
     5161 tgatgacaac gatctgtcgc accggcggac aatacctgga cgttgttggc ctgagcgtaa
     5221 caggtgatga taacgacgtt gagattgacg gcggtattaa tatcacccac agcgaggatc
//