From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Mouse mammary tumour virus (MMTV) proviral gag gene with IS1 and
Date: 22 Dec 92 22:55:28 GMT
Distribution: bionet
Checksum: 33296 5

LOCUS       MMTGA        1125 bp ss-RNA             VRL       30-NOV-1992
DEFINITION  Mouse mammary tumour virus (MMTV) proviral gag gene with IS1 and
            IS2 (NT 1500-2625).
ACCESSION   X04596
KEYWORDS    gag protein; insertion sequence; proviral gene.
SOURCE      Endogenous mouse mammary tumor virus.
  ORGANISM  Mouse mammary tumor virus
            Viridae; ss-RNA enveloped viruses; Positive strand RNA virus;
            Retroviridae; Oncovirinae; Type B oncovirus group; Mammalian type B
            oncoviruses.
REFERENCE   1  (bases 1 to 1125)
  AUTHORS   Brookes,S., Placzek,M., Moore,R., Dixon,M., Dickson,C. and
            Peters,G.
  TITLE     Insertion elements and transitions in cloned mouse mammary tumour
            virus DNA: further delineation of the poison sequences
  JOURNAL   Nucleic Acids Res. 14, 8231-8245 (1986)
  STANDARD  full automatic
COMMENT     [1]  enum. 1500 to 2625.
               *source: clone=507; strain=BR6(mouse);
               19 specific G to A transitions have been detected in clone 501,
            4 of them causing 2 stop codons (tAA; pos. 178/179 and 607/608);
            (pos. of G to A transitions: 150, 178, 179, 227, 384, 420, 465, 504
            520, 544,549, 607, 608, 768, 788, 796, 832, 1013, 1044)
               For complete sequence of MMTV provirus see also Journal of
            Virology 61:480-490(1987)
               Data kindly reviewed (11-MAY-1987) by Peters G.

              EMBL features not translated to GenBank features:
              key        from     to       description

              PROVRL        1   1125       provirus fragment

              SITE          9      9       gag gene precursor (Pr77) start site

              SITE        815    815       major core protein p27 start site
              SITE        438    582       region of poison sequence (IS rich
                                           sequence)
              SITE        439    438 (C)   IS2 junction (clone 1101)
              SITE        467    468       IS1 junction (clone 709)
              SITE        492    491 (C)   IS1 junction (clone 705)
              SITE        500    501       IS2 junction (clone 924)
              SITE        554    553 (C)   IS1 junction (clone 903)
              SITE        581    582       IS1 junction (clone 711).
FEATURES             Location/Qualifiers
     CDS             9..1125
                     /partial
                     /partial
                     /note="precursor polypeptide;  (Pr77; gag gene)(AA 1-372)
                     (1125 is 1st base in codon)"
                     /codon_start=1
                     /translation="MGVSGSKGQKLFVSVLQRLLSERGLHVKESSAIEFYQFLIKVSP
                     WFPEEGGLNLQDWKRVGREMKRYAAEHGTDSIPKQAYPIWLQLREILTEQSDLVLLSA
                     EAKSVTEEELEEGLTGLLSTSSQEKTYGTRGTAYAEIDTEVDKLSEHIYDEPYEEKEK
                     ADKNEEKDHVRKIKKVVQRKENSEGKRKEKDSKAFLATDWNDDDLSPEDWDDLEEQAA
                     HYHDDDELILPVKRKVVKKKPQALRRKPLPPVGFAGAMAEAREKGDLTFTFPVVFMGE
                     SDEDDTPVWEPLPLKTLKELQSAVRTMGPSAPYTLQVVDMVASQWLTPSDWHQTARAT
                     LSPGDYVLWRTEYEEKSKEMVQKAAGKRKGKVSLDMLL"
BASE COUNT      377 a    190 c    309 g    249 t
ORIGIN      
        1 cattggaaat gggggtctcg ggatcaaaag ggcagaaact ctttgtttct gttctacaaa
       61 gactcctctc agagaggggt cttcatgtga aagagagcag tgcaatagag ttttatcagt
      121 tcctaataaa ggtctctcct tggtttcccg aagaaggagg attaaattta caagattgga
      181 aaagggtggg aagagagatg aagaggtacg cagcagaaca tgggacggat agtataccaa
      241 agcaggctta ccccatttgg cttcagttga gagagatact gacagagcaa tcagacttgg
      301 ttttgttatc tgcagaagcc aagtccgtga ctgaagagga attagaggaa ggtttaaccg
      361 gactactatc gacaagttca caggaaaaaa cttatgggac taggggaaca gcatatgcag
      421 aaatagatac agaggtagac aagctgtctg aacatattta tgatgaacca tatgaagaaa
      481 aggagaaggc agataaaaat gaggaaaagg accatgttag aaaaataaag aaggtagtac
      541 aaagaaaaga aaatagtgag ggtaagagaa aagagaagga ttcaaaggcc tttttagcca
      601 cagattggaa cgatgatgac ctgtcccctg aggattggga cgatttagag gaacaagcgg
      661 cacattatca tgatgatgat gagctaatcc ttccagtaaa aaggaaggtg gttaagaaga
      721 aacctcaggc actcagaagg aaacccctgc ctccggtggg ttttgcagga gcgatggcag
      781 aggccaggga aaaaggagat ttgactttta cgtttcctgt agtttttatg ggagagagtg
      841 atgaagatga cacgcctgtt tgggaaccgc tgccattgaa aaccttaaag gaattgcaat
      901 cggcagttag gaccatggga ccatctgctc cctacaccct gcaggtggta gacatggtgg
      961 ctagtcaatg gctcaccccg agtgactggc accaaacagc cagagctacc ttgtctcctg
     1021 gagactatgt tttatggaga actgaatatg aagagaaaag taaagaaatg gtacaaaagg
     1081 ctgcaggcaa gcgaaagggc aaggtctctc ttgatatgtt actgg
//