From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Mouse Ins DNA; cellular component that mediates integration and
Date: 24 Jul 92 10:08:30 GMT
Distribution: bionet
Checksum: 30856     4

LOCUS       MUSINS       1628 bp ds-DNA             ROD       24-JUL-1992
DEFINITION  Mouse Ins DNA; cellular component that mediates integration and
            excision of polyoma virus DNA.
ACCESSION   K02592
KEYWORDS    .
SOURCE      Mouse Cyp cell line DNA.
  ORGANISM  Mus musculus
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Rodentia; Myomorpha; Muridae; Murinae.
REFERENCE   1  (bases 1 to 1628)
  AUTHORS   Bourgaux,P., Bourgaux-ramoisy,D., Roy,G., Allard,D. and Sylla,B.S.
  TITLE     A mouse DNA sequence that mediates integration and excision of
            polyoma virus DNA
  JOURNAL   Gene 29, 343-350 (1984)
  STANDARD  full staff_review
COMMENT     Draft entry and sequence on floppy kindly provided by P. Bourgaux,
            May 1985.
            In mouse cells, transformed by a temperature-sensitive polyoma
            virus genome, the integrated viral genome recombines with adjacent
            chromosomal DNA (Ins) upon excision to yield a small cyclic
            molecule (RmI) with defined viral and cellular components, capable
            of autonomous replication in mouse cells and able to transform rat
            cells.  Enhancer sequences were found at bp 332-341, 370-378,
            998-1006, 1150-1158, and 1203-1211; a TATA box at 161-172; and
            poly-A signals at 796-801 and 318-323; a CAAT box from 94-107; and
            a ribosome binding site at 333-337.  Bases 664-777 were found to be
            homologous to mouse repetitive element B2.
FEATURES             Location/Qualifiers
     misc_recomb     <1..1
                     /note="polyoma DNA end/mouse DNA (Ins) start"
BASE COUNT      490 a    334 c    364 g    434 t      6 others
ORIGIN      88 bp upstream of AluI site.
        1 tgtgcctagg atgttaatgt actgtgagta gggcatgacg tgtaaagtat cagcgtgcca
       61 gtattaaaag catgtattaa tgcttcagct aatgttttgg tcaatgcatt ttagttttcg
      121 aatcattaaa ttactgtgtc ccataagaat aagacctatt tataactata tacttattgt
      181 agtaccaata ttagataaat ttgttactgg catgcattgt caaagaaagc cagaaaggat
      241 gttagatcct gtcttagtca gggtttgtat tcttgcacaa aacntcntgn ccaagaagca
      301 agttggggag gaaagggttt attcagctta cacttccaca ttgctgatca tcaccaaagg
      361 aagcagaacg aaccacacag ggcaggaact tggagggcag gagctgatgc agaggccgtg
      421 aaggggtgct gcttactggc ttgcttccct tggcttgctc cgcctgattt cttatagaat
      481 caggactacc agcccaggga tggcaccact tacaatgggc tgggcccncc tncctngatc
      541 actaattgag aaaatgcctt acagctggat ctcatggacg cattttctca agggaggttt
      601 cttttgctgt gataacccca gcttgcgaca agtcggcaca cagaaccagc cagtacagat
      661 ctctaggaac tggagttaca gatgactgtg agccgccatg tgggtgctgg gaattgaacc
      721 caggtcctct ggaagagcag tccatgctcc taactcctga gccatctctc cctctctgtc
      781 tctcctctct gcatgtttat ttgtaacttc tgtctccagc tgtacaaaat ttgactctcc
      841 tctataactt atatacttat ttgttcaact ctagtgttga gcatgaaatt atttctaaca
      901 taattatcct ccctataggg aaagtcctac caataggaag cttaacacgc atttttgtca
      961 ttggtctcac agagtggagt caaaatgttg ctttgcaaaa ccacagaggc caaaagcaat
     1021 cattcaaagg tgtgacacaa attcacactg cccatgttct ctaggtaggt aacaccacag
     1081 tgaggatccc acggggataa ttgctacagt gggttttgtg ttcatcaaaa tggaagaatg
     1141 ccatgcgtat aaccacagtg gcaagcagag ggctctgggt ggcttagcag aagggactaa
     1201 cccaaccaca gcgatgtaaa atgtctatgt tatatacaaa gggtgtgaat gtaacaccaa
     1261 gagaaaacag tgtctaactg tgatccccag agcaagcctt ccgaaataat gacaaataat
     1321 tcaacttgaa agggcaccag atgcatcaaa atgggattct gaaatgtgct caaactgtgt
     1381 gaagggggaa agaaaatgag aagagaggaa gaaaattccc aaactggatc aaaccagaac
     1441 aacagcaaag ttggaattgt cagatggagg ccaaatgatg aaatggtaga cctaattcca
     1501 atcatattaa taattgaatg ggaatatatc attcggacca ttaagaagtt gctattcctt
     1561 gataatgtga ggagagtgag aggcatctaa atgggacgtc cttatcacat tcctcctttc
     1621 aaggctca
//