From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Clostridium acetobutylicum butanol dehydrogenase isozyme B gene,
Date: 29 Jan 93 05:45:39 GMT
Distribution: bionet
Checksum: 38620 3

LOCUS       CLOBDB       1173 bp ds-DNA             BCT       28-JAN-1993
DEFINITION  Clostridium acetobutylicum butanol dehydrogenase isozyme B gene,
            complete cds.
ACCESSION   M96946
KEYWORDS    butanol dehydrogenase; isozyme.
SOURCE      Clostridium acetobutylicum (library: ATCC 824) DNA.
  ORGANISM  Clostridium acetobutylicum
            Prokaryotae; Firmicutes; Endospore-forming rods and cocci;
            Bacillaceae.
REFERENCE   1  (bases 1 to 1173)
  AUTHORS   Walter,K.A., Bennett,G.N. and Papoutsakis,E.T.
  TITLE     Molecular characterization of two Clostridium acetobutylicum ATCC
            824 butanol dehydrogenase isozyme genes
  JOURNAL   J. Bacteriol. 174, 7149-7158 (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     CDS             1..1173
                     /note="isozyme B"
                     /product="butanol dehydrogenase"
                     /codon_start=1
                     /translation="MVDFEYSIPTRIFFGKDKINVLGRELKKYGSKVLIVYGGGSIKR
                     NGIYDKAVSILEKNSIKFYELAGVEPNPRVTTVEKGVKICRENGVEVVLAIGGGSAID
                     CAKVIAAACEYDGNPWDIVLDGSKIKRVLPIASILTIAATGSEMDTWAVINNMDTNEK
                     LIAAHPDMAPKFSILDPTYTYTVPTNQTAAGTADIMSHIFEVYFSNTKTAYLQDRMAE
                     ALLRTCIKYGGIALEKPDDYEARANLMWASSLAINGLLTYGKDTNWSVHLMEHELSAY
                     YDITHGVGLAILTPNWMEYILNNDTVYKFVEYGVNVWGIDKEKNHYDIAHQAIQKTRD
                     YFVNVLGLPSRLRDVGIEEEKLDIMAKESVKLTGGTIGNLRPVNASEVLQIFKKSV"
BASE COUNT      452 a    151 c    254 g    316 t
ORIGIN      
        1 gtggttgatt tcgaatattc aataccaact agaatttttt tcggtaaaga taagataaat
       61 gtacttggaa gagagcttaa aaaatatggt tctaaagtgc ttatagttta tggtggagga
      121 agtataaaga gaaatggaat atatgataaa gctgtaagta tacttgaaaa aaacagtatt
      181 aaattttatg aacttgcagg agtagagcca aatccaagag taactacagt tgaaaaagga
      241 gttaaaatat gtagagaaaa tggagttgaa gtagtactag ctataggtgg aggaagtgca
      301 atagattgcg caaaggttat agcagcagca tgtgaatatg atggaaatcc atgggatatt
      361 gtgttagatg gctcaaaaat aaaaagggtg cttcctatag ctagtatatt aaccattgct
      421 gcaacaggat cagaaatgga tacgtgggca gtaataaata atatggatac aaacgaaaaa
      481 ctaattgcgg cacatccaga tatggctcct aagttttcta tattagatcc aacgtatacg
      541 tataccgtac ctaccaatca aacagcagca ggaacagctg atattatgag tcatatattt
      601 gaggtgtatt ttagtaatac aaaaacagca tatttgcagg atagaatggc agaagcgtta
      661 ttaagaactt gtattaaata tggaggaata gctcttgaga agccggatga ttatgaggca
      721 agagccaatc taatgtgggc ttcaagtctt gcgataaatg gacttttaac atatggtaaa
      781 gacactaatt ggagtgtaca cttaatggaa catgaattaa gtgcttatta cgacataaca
      841 cacggcgtag ggcttgcaat tttaacacct aattggatgg agtatatttt aaataatgat
      901 acagtgtaca agtttgttga atatggtgta aatgtttggg gaatagacaa agaaaaaaat
      961 cactatgaca tagcacatca agcaatacaa aaaacaagag attactttgt aaatgtacta
     1021 ggtttaccat ctagactgag agatgttgga attgaagaag aaaaattgga cataatggca
     1081 aaggaatcag taaagcttac aggaggaacc ataggaaacc taagaccagt aaacgcctcc
     1141 gaagtcctac aaatattcaa aaaatctgtg taa
//