From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Rattus rattus phosphorylase kinase gene, exons 2 and 3.
Date: 15 Aug 92 10:03:05 GMT
Distribution: bionet
Checksum: 49545     5

LOCUS       RATPHOSKIN   1542 bp ds-DNA             ROD       15-AUG-1992
DEFINITION  Rattus rattus phosphorylase kinase gene, exons 2 and 3.
ACCESSION   M98826
KEYWORDS    catalytic subunit; phosphorylase kinase.
SOURCE      Rattus norvegicus (strain Wistar) (library: Stratagene;lambda DASH)
            DNA.
  ORGANISM  Rattus norvegicus
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Rodentia; Myomorpha; Muridae; Murinae.
REFERENCE   1  (sites)
  AUTHORS   Cawley,K.C., Ramachandran,C., Gorin,F.A. and Walsh,D.A.
  TITLE     Nucleotide sequence of cDNA encoding the catalytic subunit of
            phosphorylase kinase from rat soleus muscle
  JOURNAL   Nucleic Acids Res. 16, 2355-2356 (1988)
  STANDARD  full staff_review
REFERENCE   2  (bases 1 to 1542)
  AUTHORS   Cawley,K.C., Akita,C.G., Angelos,K.L. and Walsh,D.A.
  TITLE     Characterization of the gene for rat phosphorylase kinase catalytic
            subunit
  JOURNAL   Unpublished (1992)
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     intron          <1..730
                     /number=1
                     /cons_splice=(5'site:no,3'site:yes)
                     /note="entire intron 1 is ~3.3kb"
                     /evidence=EXPERIMENTAL
                     /citation=[2]
                     /citation=[1]
     CDS             join(764..846,1263..1441)
                     /product="phosphorylase kinase gamma subunit"
                     /codon_start=1
                     /translation="MTRDEALPDSHSAQNFYENYEPKEILGRGVSSVVRRCIHKPTCQ
                     EYAVKIIDITGGGSFSSEEVQELREATLKEVDILQKVSGHPNI"
                     /partial
     exon            731..846
                     /number=2
                     /evidence=EXPERIMENTAL
                     /citation=[2]
                     /citation=[1]
     intron          847..1262
                     /number=2
                     /evidence=EXPERIMENTAL
                     /citation=[2]
                     /citation=[1]
     exon            1263..1441
                     /number=3
                     /evidence=EXPERIMENTAL
                     /citation=[2]
                     /citation=[1]
     intron          1442..1542
                     /number=3
                     /cons_splice=(5'site:yes,3'site:no)
                     /evidence=EXPERIMENTAL
                     /citation=[2]
                     /citation=[1]
                     /partial
BASE COUNT      449 a    345 c    414 g    334 t
ORIGIN
        1 cggtgtgatg tatgcagaag ttcgtcccgg aaggagaggc aggaagggag tccagaggga
       61 tggaagagta ccctggggat ggaattgtac cagcaggaaa gtgggaagct atccttcttt
      121 gccacaagtg cagagaaaac acagaagaaa agcgagggtt ggagagatgg ctcagcagtt
      181 agcagtggaa gcccttgcag tcgccagagt ttggttccca gcacctgcat ggtggctcac
      241 aactgctcta atccagtatc tcgggctcct tctttatcca tcttctctcc tccgcaggca
      301 tctgcattcg tgcgcacatg cccacagaca gcattgtgtg ccttaaaaaa aaaaaaaata
      361 tttcaagaca gtgcttctct gttttagctc tgcgtatttt ggaactacag cggtggccca
      421 ggcctttaat ctcagcaccc aggaggcaaa ggtggcaaat ctctgtgagt ttgaggccga
      481 ctggtctaca gaaatagttc caggacagct ggaagtaaaa cacaaaaaac cctgtcatat
      541 ggaaaaaaga aagaaaggaa aagaaaagaa aagaaaagaa aaagaaagaa aggaaggaag
      601 aaagaaagaa aagagagagg aaagaaagaa aagagaaagg aaagaaagga ggaaggaaaa
      661 gaaaagaaag aaaaaggggg aaaaaggaag gaggagtggg aatttcctct tctttctgaa
      721 tccttttcag aggtgcttgg gatcccctga gatccagctg accatgaccc gggatgaggc
      781 cctccctgac tctcattctg cacagaattt ctatgagaac tacgagccca aggagatcct
      841 gggcaggtaa gactcggacc acccgggaag tcctgaccgc acctctgctc tgatcgctga
      901 ttggctcacc ctctgctcta acgggatttc tgacccttag ctctccttag aatgagaagg
      961 gaagtctgtg ttacaaccca gggccttcga attctgattg atttaatttc tgttttctga
     1021 gtacaagtgt ttcggccgta tgcagtttgt gcgccacatg cgtgtctggt gtccttagag
     1081 gtcagagtag ctgtcggatc ccctggattt ccagacagtt gttagtagcc atgtgggtgc
     1141 cgggaactga acccaggttc tctgaaagaa caagtactca gtctctgagc tttctctcca
     1201 gtccctttgg taagggagta tggagtatgg agtccccagg ccctctgttc tctgcccaca
     1261 aggggagtca gcagcgtggt caggagatgc attcacaaac ctacgtgcca agaatacgca
     1321 gtaaaaatca tcgacatcac cggcggagga agctttagct ccgaggaggt acaggagctt
     1381 cgggaagcca ccctgaagga ggtggacatt ctgcagaagg tctcaggaca ccccaacatc
     1441 agtaagtgta tcctccaaac tccccaactc ccctgggcac aaagcagggc agcgtcgttt
     1501 atagtgaatg attctgccct aataacttac gaagtcaggg tg
//