From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Giardia lamblia (clone pMM51, 4 kb BamHI fragment in pGEM-4)
Date: 29 Dec 92 01:17:14 GMT
Distribution: bionet
Checksum: 65195 4

LOCUS       GIATRIISOB   1701 bp ds-DNA             INV       07-DEC-1992
DEFINITION  Giardia lamblia (clone pMM51, 4 kb BamHI fragment in pGEM-4)
            triosephosphate isomerase gene, complete cds.
ACCESSION   L02116
KEYWORDS    triosephosphate isomerase.
SOURCE      Giardia lamblia (individual_isolate GS/M) (library: GS/H7 Sau3A
            partial genomic in lambda-FixII) trophozoite DNA.
  ORGANISM  Giardia lamblia
            Eukaryota; Animalia; Protozoa; Sarcomastigophora; Mastigophora;
            Zoomastigophora; Diplomonadida; Diplomonadina; Hexamitidae.
REFERENCE   1  (bases 1 to 1701)
  AUTHORS   Mowatt,M.R., Howard,T.C. and Nash,T.E.
  TITLE     Divergence of triosephosphate isomerase gene and protein sequences
            between independent Giardia lamblia isolates
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     5'UTR           534..542
                     /citation=[1]
                     /note="determined by primer extension sequence analysis"
     polyA_signal    1363..1369
                     /note="putative"
     CDS             543..1316
                     /EC_number="5.3.1.1"
                     /product="triosephosphate isomerase"
                     /codon_start=1
                     /translation="MPARRPFIGGNFKCNGSLDFIKSHVASIASYKIPESVDVVVAPS
                     FVHLSTAIAANTSKCLKIAAQNVYLEGNGAWTGETSVEMLLDMGLSHVIIGHSERRRI
                     MGETNEQSAKKAKRALDKGMTVIFCTGETLDERKANNTMEVNIAQLEALKKEIGESKK
                     LWENVVIAYEPVWSIGTGVVATPEQAEEVHVGLRKWFAEKVCAEGAQHIRIIYGGSAN
                     GSNCEKLGQCPNIDGFLVGGASLKPEFTTMIDILAKTRA"
BASE COUNT      455 a    375 c    436 g    435 t
ORIGIN      
        1 ggacaaatgc accgacccat tgcctatcat accctggatg gagattccca tttcaggcgc
       61 caagtggtct ctgaaggagg ctgttgaagc ccttcaatcc cacatctcag atagtaacgc
      121 atttgtacga aactcgggtt tatcagagcg cccgtttgga cggccatcct atgatcctgt
      181 tatctccaaa ggaatatatg tgctagcact atctaatgat gcgcttcatg ccactattct
      241 tcatgcttgt atcagcgaat gtaactctgt agtttttctt ctgttctgct tcttagatca
      301 gaatgcagag gctagcaagc ttctggcacg gcacagcaat gttattatat ttgatattct
      361 ccgacctaac tatactcaac ttcagcatgt acttctatcc atctgtggct gcattcgtta
      421 cagtgctgaa agtggaaaac gaaggcaatg catctgtagc tatcatgcta aataaaaggc
      481 tagtcgttgt acagctttgg tttaaaaacc ctgattcgaa aattctgatc agaaaataaa
      541 ttatgcctgc tcgtcgtccc ttcatcggtg gtaacttcaa atgcaatgga tcgctcgact
      601 tcattaagag ccacgtagcg tccatcgcct cctataagat ccccgagtcc gtggacgttg
      661 ttgttgctcc ctcctttgtg cacctttcta cagctattgc ggcgaatact tcgaagtgtc
      721 tgaaaatagc agcacagaac gtgtatctgg aagggaacgg tgcatggacc ggcgagacaa
      781 gcgtcgagat gctgctggac atggggctga gccatgtaat aataggacac tctgaaagac
      841 gtagaatcat gggcgagacc aatgagcaga gtgctaagaa ggcgaagcgt gctctggaca
      901 aaggtatgac tgttatcttc tgcaccggag agaccctgga tgaacgcaag gccaataaca
      961 ctatggaggt gaatattgct cagctcgagg ctcttaagaa ggagattgga gaatcaaaga
     1021 agttatggga gaacgttgta attgcctatg agccggtgtg gtctatcggc acgggtgtgg
     1081 tggccacacc ggagcaggca gaggaagtcc atgtgggact ccgcaaatgg tttgcggaaa
     1141 aggtttgcgc agaaggtgcg cagcacatcc gcatcatcta tggagggtct gccaatggga
     1201 gtaactgcga gaagcttggc cagtgcccga atatcgacgg attcctcgtc ggaggtgctt
     1261 ccctcaagcc ggaatttaca acgatgattg atattctcgc gaagactcgt gcatagacac
     1321 tctgcaaact ctgaaagctt ttacttgcag tacatttcaa tgagtaaatt gacaaggtac
     1381 cgacttcaac ccggatgctt gattagttgg tccatcacat ctgattgcat ttttatggga
     1441 ttgagatata tgagaccacg ctcagttgtt agctatacga ctacgtggta ctccgcatac
     1501 actgtggctt gagtagattg tggtggagta gagcctcaat tggaaccagc aggtggcgtc
     1561 agtgccttga taggagcaat cttgtgcaca cccaggacaa atgaaaggat aacgaaagca
     1621 gaggaaaagg atgattgggc gctgatcaat gtgtagaagt aactggtagt gatcccagta
     1681 catgcaagac ctgcggggcc g
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