From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Myxoma virus MVF8 gene for thymidine kinase, MVF6 gene (partial
Date: 21 Jul 92 10:07:39 GMT
Distribution: bionet
Checksum: 13382     5

LOCUS       MYXMVF       1866 bp ds-DNA             VRL       21-JUL-1992
DEFINITION  Myxoma virus MVF8 gene for thymidine kinase, MVF6 gene (partial
            cds), MVF7 gene, and MV8b gene
ACCESSION   X52655 X58790
KEYWORDS    MV8b gene; MVF6 gene; MVF7 gene; MVF8 gene; thymidine kinase.
SOURCE      Myxoma virus DNA.
  ORGANISM  Myxoma virus
            Viridae; ds-DNA enveloped viruses; Poxviridae; Chordopoxvirinae.
REFERENCE   1  (bases 1 to 1638; 1259 to 1866)
  AUTHORS   Jackson,R.J.
  JOURNAL   Unpublished (1990)
  STANDARD  full automatic
REFERENCE   2  (sites)
  AUTHORS   Jackson,R.J. and Bults,H.G.
  TITLE     The Myxoma virus thymidine kinase gene: sequence and
            transcriptional mapping
  JOURNAL   J. Gen. Virol. 73, 323-328 (1992)
  STANDARD  full staff_review
COMMENT     *source: clone=pUrTK1, pUrSal-AA2; *source: isolate=
            AUST/URIARRA/VERG-86/1;
            From EMBL    entry MVMVF;  dated 12-JUN-1992.
FEATURES             Location/Qualifiers
     CDS             <1..291
                     /gene="MVF6"
                     /codon_start=1
                     /translation="ELAVIALLIFLFFKTEFNMLLRNTVDLANDPIKQFTSTSLTCKG
                     NALFIKHMPNSTELKPALAINRKQIVHENCAALLQSINGSRKVSLNDILERR"
     CDS             245..691
                     /gene="MVF7"
                     /codon_start=1
                     /translation="MDHGKYLLTIFLNDDDSFFKYLAAQEDDVAMSDVHTIVDYLNFL
                     LALLIKSKDKLEAVGYYYAPLSEEYKAVFDFTDTKSLKQLFNKQPVYVESDSPICVDK
                     GYLADFVLATTRLKKQLPLVLDKEVTYVDPYKDKRFANILSILHKN"
     CDS             724..1260
                     /EC_number="2.7.1.21"
                     /product="thymidine kinase"
                     /gene="MVF8"
                     /codon_start=1
                     /translation="MAMYGGQIHLIIGPMFAGKSTELIRLVKRYQIARYKCLVVNYEK
                     DARYGNGVRTHDNTCISAVPTASLDDVESISEHVEVIGIDEGQFFPNIVSFCERMANA
                     GKVLIVAALDGTFQRKPFTNICELIPLAENVTKLNAVCMYCYKDASFSKRLGNETEIE
                     IIGGSDKYKSVCRKCYFF"
     promoter        1260..1275
     CDS             1333..1671
                     /gene="MV8b"
                     /codon_start=1
                     /translation="MGVQHKLDIFLVSEGIAIKEANLLKGDSYGCTIKIKLDKEKTFK
                     FVIVLEPEWIDEIKPIYMKVNDESVELELDYKDAIKRIYSAEVVLCSDSVINLFSDVD
                     RVLYVRIPYD"
     polyA_signal    1811..1818
     polyA_signal    1813..1819
BASE COUNT      601 a    365 c    422 g    478 t
ORIGIN
        1 gagctcgcag tgatcgcttt gttgattttc ctctttttca aaaccgagtt taacatgtta
       61 ctgcggaaca ccgtcgatct ggcgaacgat cctataaaac agtttacctc aacgagtttg
      121 acgtgtaagg gaaacgcgct atttatcaaa cacatgccta attcgacgga gttaaaaccg
      181 gcgctcgcga ttaatagaaa acaaatagtc cacgagaact gcgcggctct tttacaatca
      241 ataaatggat cacggaaagt atctcttaac gatattcttg aacgacgatg attcgttctt
      301 caagtatttg gcggcacagg aggacgacgt cgcgatgtcg gacgttcaca cgatcgtaga
      361 ctatttaaac tttttactag ccctattaat taaatcgaaa gataaactag aagccgtagg
      421 atattattac gctcctctgt cggaggaata caaagccgtg tttgatttca ccgacacaaa
      481 gtcgttgaaa caattgttta acaaacaacc cgtgtacgtt gagagcgact ctcccatttg
      541 cgtggataag ggatatttgg cagattttgt tctcgcaacg actagattaa agaaacaatt
      601 acctttggtg ttggataagg aagttacgta cgtagatccg tataaggata aacgattcgc
      661 aaatatcctg tctatattgc ataaaaactg aagcattaaa aactattatt acaagtttaa
      721 atcatggcca tgtacggggg acagattcac ctcattatag gacccatgtt tgccggtaaa
      781 agcacggaac taatccgtct agttaaacgt tatcagatag cgagatacaa atgtctcgtt
      841 gtaaattacg aaaaagacgc ccgttacgga aacggcgtac gcactcacga taacacatgc
      901 atctccgccg taccaaccgc gtccctagac gacgtagaat cgatatccga acacgtcgaa
      961 gtgatcggga tagacgaggg acagtttttc cccaacatcg tatcgttttg cgaacgtatg
     1021 gcgaatgccg gaaaggtgtt gatcgtggcc gcgttggacg gaacgtttca acgtaaaccg
     1081 tttaccaaca tttgtgaact gataccgttg gccgaaaacg taacaaaact gaacgcggta
     1141 tgtatgtact gttacaagga cgcatctttc tctaaacggt tgggaaacga gacagagatc
     1201 gaaataatag gaggtagcga caagtacaaa tcggtgtgta gaaaatgtta ttttttttaa
     1261 aatactgaaa aaataaatcg gatatttacg attaggttga tcgtctccga cagcgacctc
     1321 taggacgtcg gtatgggcgt gcaacacaaa ttggacatat tcctcgtgag cgaagggatc
     1381 gccatcaaag aggcaaatct actcaagggg gacagttacg ggtgcaccat caaaataaaa
     1441 ttggataagg aaaagacgtt caaattcgtc atcgtattgg aaccggagtg gatagatgag
     1501 ataaaaccta tatacatgaa agttaacgac gagtcggtgg agttagaatt agactataaa
     1561 gacgccatca aacgcatcta ttcggcggag gtggtattat gttcagattc cgtgatcaac
     1621 ctgttcagtg acgtcgaccg tgtcttatac gtgcgaatac cctacgatta aggtgaatac
     1681 gataaaaaaa tactacagcg tacagaacag agggatgacc tacgtacata tagaatcgcc
     1741 cattaatacg aaagataaat gctggttcgt ggaaaagaac ggatggtacg aggatagaac
     1801 acattcgtaa tttttttata tagtgaaaaa taatgtgagc attacgagcg tgggtttatc
     1861 tacacg
//