From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Kluyveromyces lactis beta-D-galactosidase (LAC4) gene, complete
Date: 15 Sep 92 10:06:33 GMT
Distribution: bionet
Checksum: 38717     8

LOCUS       YSKLAC4A     3703 bp ds-DNA             PLN       15-SEP-1992
DEFINITION  Kluyveromyces lactis beta-D-galactosidase (LAC4) gene, complete
            cds.
ACCESSION   M84410
KEYWORDS    LAC4 gene; beta-D-galactosidase.
SOURCE      Kluyveromyces lactis (library: CBS2359) DNA.
  ORGANISM  Kluyveromyces lactis
            Eukaryota; Fungi; Eumycota; Hemiascomycetes; Endomycetales;
            Saccharomycetaceae.
REFERENCE   1  (bases 1 to 3703)
  AUTHORS   Poch,O., L'Hote,H., Dallery,V., Debeaux,F., Fleer,R. and Sodoyer,R.
  TITLE     Sequence of the Kluyveromyces lactis beta-galactosidase: Comparison
            with prokaryotic enzymes and secondary structure analysis
  JOURNAL   Gene 118, 55-63 (1992)
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     TATA_signal     9..12
                     /gene="LAC4"
                     /note="putative"
     CDS             43..3120
                     /EC_number="3.2.1.23"
                     /product="beta-D-galactosidase"
                     /gene="LAC4"
                     /codon_start=1
                     /translation="MSCLIPENLRNPKKVHENRLPTRAYYYDQDIFESLNGPWAFALF
                     DAPLDAPDAKNLDWETAKKWSTISVPSHWELQEDWKYGKPIYTNVQYPIPIDIPNPPT
                     VNPTGVYARTFELDSKSIESFEHRLRFEGVDNCYELYVNGQYVGFNKGSRNGAEFDIQ
                     KYVSEGENLVVVKVFKWSDSTYIEDQDQWWLSGIYRDVSLLKLPKKAHIEDVRVTTTF
                     VDSQYQDAELSVKVDVQGSSYDHINFTLYEPEDGSKVYDASSLLNEENGNTTFSTKEF
                     ISFSTKKNEETAFKINVKAPEHWTAENPTLYKYQLDLIGSDGSVIQSIKHHVGFRQVE
                     LKDGNITVNGKDILFRGVNRHDHHPRFGRAVPLDFVVRDLILMKKFNINAVRNSHYPN
                     HPKVYDLFDKLGFWVIDEADLETHGVQEPFNRHTNLEAEYPDTKNKLYDVNAHYLSDN
                     PEYEVAYLDRASQLVLRDVNHPSIIIWSLGNEACYGRNHKAMYKLIKQLDPTRLVHYE
                     GDLNALSADIFSFMYPTFEIMERWRKNHTDENGKFEKPLILCEYGHAMGNGPGSLKEY
                     QELFYKEKFYQGGFIWEWANHGIEFEDVSTADGKLHKAYAYGGDFKEEVHDGVFIMDG
                     LCNSEHNPTPGLVEYKKVIEPVHIKIAHGSVTITNKHDFITTDHLLFIDKDTGKTIDV
                     PSLKPEESVTIPSDTTYVVAVLKDDAGVLKAGHEIAWGQAELPLKVPDFVTETAEKAA
                     KINDGKRYVSVESSGLHFILDKLLGKIESLKVKGKEISSKFEGSSITFWRPPTNNDEP
                     RDFKNWKKYNIDLMKQNIHGVSVEKGSNGSLAVVTVNSRISPVVFYYGFETVQKYTIF
                     ANKINLNTSMKLTGEYQPPDFPRVGYEFWLGDSYESFEWLGRGPGESYPDKKESQRFG
                     LYDSKDVEEFVYDYPQENGNHTDTHFLNIKFEGAGKLSIFQKEKPFNFKISDEYGVDE
                     AAHACDVKRYGRHYLRLDHAIHGVGSEACGPAVLDQYRLKAQDFNFEFDLAFE"
     polyA_signal    3401..3406
                     /gene="LAC4"
BASE COUNT     1156 a    678 c    793 g   1076 t
ORIGIN
        1 aaaaaaaaaa taaacacaca tactcatcga gaactgaaag atatgtcttg ccttattcct
       61 gagaatttaa ggaaccccaa aaaggttcac gaaaatagat tgcctactag ggcttactac
      121 tatgatcagg atattttcga atctctcaat gggccttggg cttttgcgtt gtttgatgca
      181 cctcttgacg ctccggatgc taagaattta gactgggaaa cggcaaagaa atggagcacc
      241 atttctgtgc catcccattg ggaacttcag gaagactgga agtacggtaa accaatttac
      301 acgaacgtac agtaccctat cccaatcgac atcccaaatc ctcccactgt aaatcctact
      361 ggtgtttatg ctagaacttt tgaattagat tcgaaatcga ttgagtcgtt cgagcacaga
      421 ttgagatttg agggtgtgga caattgttac gagctttatg ttaatggtca atatgtgggt
      481 ttcaataagg ggtcccgtaa cggggctgaa tttgatatcc aaaagtacgt ttctgagggc
      541 gaaaacttag tggtcgtcaa ggttttcaag tggtccgatt ccacttatat cgaggaccaa
      601 gatcaatggt ggctctctgg tatttacaga gacgtttctt tactaaaatt gcctaagaag
      661 gcccatattg aagacgttag ggtcactaca acttttgtgg actctcagta tcaggatgca
      721 gagctttctg tgaaagttga tgtccagggt tcttcttatg atcacatcaa tttcacactt
      781 tacgaacctg aagatggatc taaagtttac gatgcaagct ctttgttgaa cgaggagaat
      841 gggaacacga ctttttcaac taaagaattt atttccttct ccaccaaaaa gaacgaagaa
      901 acagctttca agatcaacgt caaggcccca gaacattgga ccgcagaaaa tcctactttg
      961 tacaagtacc agttggattt aattggatct gatggcagtg tgattcaatc tattaagcac
     1021 catgttggtt tcagacaagt ggagttgaag gacggtaaca ttactgttaa tggcaaagac
     1081 attctcttta gaggtgtcaa cagacatgat caccatccaa ggttcggtag agctgtgcca
     1141 ttagattttg ttgttaggga cttgattcta atgaagaagt ttaacatcaa tgctgttcgt
     1201 aactcgcatt atccaaacca tcctaaggtg tatgacctct tcgataagct gggcttctgg
     1261 gtcattgacg aggcagatct tgaaactcat ggtgttcaag agccatttaa tcgtcatacg
     1321 aacttggagg ctgaatatcc agatactaaa aataaactct acgatgttaa tgcccattac
     1381 ttatcagata atccagagta cgaggtcgcg tacttagaca gagcttccca acttgtccta
     1441 agagatgtca atcatccttc gattattatc tggtccttgg gtaacgaagc ttgttatggc
     1501 agaaaccaca aagccatgta caagttaatt aaacaattgg atcctaccag acttgtgcat
     1561 tatgagggtg acttgaacgc tttgagtgca gatatcttta gtttcatgta cccaacattt
     1621 gaaattatgg aaaggtggag gaagaaccac actgatgaaa atggtaagtt tgaaaagcct
     1681 ttgatcttgt gtgagtacgg ccatgcaatg ggtaacggtc ctggctcttt gaaagaatat
     1741 caagagttgt tctacaagga gaagttttac caaggtggct ttatctggga atgggcaaat
     1801 cacggtattg aattcgaaga tgttagtact gcagatggta agttgcataa agcttatgct
     1861 tatggtggtg actttaagga agaggttcat gacggagtgt tcatcatgga tggtttgtgt
     1921 aacagtgagc ataatcctac tccgggcctt gtagagtata agaaggttat tgaacccgtt
     1981 catattaaaa ttgcgcacgg atctgtaaca atcacaaata agcacgactt cattacgaca
     2041 gaccacttat tgtttatcga caaggacacg ggaaagacaa tcgacgttcc atctttaaag
     2101 ccagaagaat ctgttactat tccttctgat acaacttatg ttgttgccgt gttgaaagat
     2161 gatgctggtg ttctaaaggc aggtcatgaa attgcctggg gccaagctga acttccattg
     2221 aaggtacccg attttgttac agagacagca gaaaaagctg cgaagatcaa cgacggtaaa
     2281 cgttatgtct cagttgaatc cagtggattg cattttatct tggacaaatt gttgggtaaa
     2341 attgaaagcc taaaggtcaa gggtaaggaa atttccagca agtttgaggg ttcttcaatc
     2401 actttctgga gacctccaac gaataatgat gaacctaggg actttaagaa ctggaagaag
     2461 tacaatattg atttaatgaa gcaaaacatc catggagtga gtgtcgaaaa aggttctaat
     2521 ggttctctag ctgtagtcac ggttaactct cgtatatccc cagttgtatt ttactatggg
     2581 tttgagactg ttcagaagta cacgatcttt gctaacaaaa taaacttgaa cacttctatg
     2641 aagcttactg gcgaatatca gcctcctgat ttcccaagag ttgggtacga attctggcta
     2701 ggagatagtt atgaatcatt tgaatggtta ggtcgcgggc ccggcgaatc atatccggat
     2761 aagaaggaat ctcaaagatt cggtctttac gattccaaag atgtagagga attcgtatat
     2821 gactatcctc aagaaaatgg aaatcataca gatacccact ttttgaacat caaatttgaa
     2881 ggtgcaggaa aactatcgat cttccaaaag gagaagccat ttaacttcaa gatttcagac
     2941 gaatacgggg ttgatgaagc tgcccacgct tgtgacgtta aaagatacgg cagacactat
     3001 ctaaggttgg accatgcaat ccatggtgtt ggtagcgaag catgcggacc tgctgttctg
     3061 gaccagtaca gattgaaagc tcaagatttc aactttgagt ttgatctcgc ttttgaataa
     3121 gaattttata cttagataag tatgtactta caggtatatt tctatgagat actgatgtat
     3181 acatgcatga taatatttaa acggttatta gtgccgattg tcttgtgcga taatgacgtt
     3241 cctatcaaag caatacactt accacctatt acatgggcca agaaaatatt ttcgaacttg
     3301 tttagaatat tagcacagag tatatgatga tatccgttag attatgcatg attcattcct
     3361 acaacttttt cgtagcataa ggattaatta cttggatgcc aataaaaaaa aaaaacatcg
     3421 agaaaatttc agcatgctca gaaacaattg cagtgtatca aagtaaaaaa aagattttca
     3481 ctacatgttc cttttgaaga aagaaaatca tggaacatta gatttacaaa aatttaacca
     3541 ccgctgatta acgattagac cgttaagcgc acaacaggtt attagtacag agaaagcatt
     3601 ctgtggtgtt gccccggact ttcttttgcg acataggtaa atcgaatacc atcatactat
     3661 cttttccaat gactccctaa agaaagactc ttcttcgatg ttg
//