From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: E.coli D-serine deaminase (dsdA) gene, complete cds.
Date: 22 Dec 92 22:51:05 GMT
Distribution: bionet
Checksum: 34221 6

LOCUS       ECODSDA      1989 bp ds-DNA             BCT       30-NOV-1992
DEFINITION  E.coli D-serine deaminase (dsdA) gene, complete cds.
ACCESSION   J01603 M15237 M22415
KEYWORDS    D-Serine deaminase; D-serine dehydratase; deaminase; dsdA gene.
SOURCE      Escherichia coli K12 DNA, clone pAC13.
  ORGANISM  Escherichia coli
            Prokaryotae; Gram-negative facultatively anaerobic rods;
            Enterobacteriaceae.
REFERENCE   1  (bases 144 to 1142)
  AUTHORS   McFall,E. and Runkel,L.
  TITLE     DNA sequences of the D-serine deaminase control region and
            N-terminal portion of the structural gene
  JOURNAL   J. Bacteriol. 154, 1508-1512 (1983)
  STANDARD  full automatic
REFERENCE   2  (bases 1 to 560)
  AUTHORS   Bornstein-Forst,S.M., McFall,E. and Palchaudhuri,S.
  TITLE     In vivo D-serine deaminase transcription start sites in wild-type
            Escherichia coli and in dsdA promotor mutants
  JOURNAL   J. Bacteriol. 169, 1056-1060 (1987)
  STANDARD  full automatic
REFERENCE   3  (bases 545 to 1989)
  AUTHORS   Marceau,M., McFall,E., Lewis,S.D. and Shafer,J.A.
  TITLE     D-serine dehydratase from Escherichia coli: DNA sequence and
            identification of catalytically inactive glycine to aspartic acid
            variants
  JOURNAL   J. Biol. Chem. 263, 16926-16933 (1988)
  STANDARD  full automatic
COMMENT     [2]  revises [1]. [3]  revises [2].
               Printed copy of sequence for [2] kindly provided by E.McFall,
            15-APR-1987.
               Draft entry and printed copy of sequence for [3] kindly provided
            by M.Marceau, 03-FEB-1989.
FEATURES             Location/Qualifiers
     mRNA            477..>1889
                     /note="dsda mRNA"
     mat_peptide     558..998
                     /codon_start=1
                     /note="D-serine deaminase"
     mutation        1393
                     /note="g in wt; a in EM1201"
     mutation        1399
                     /note="g in wt; a in AC6082"
     CDS             214..540
                     /note="ORF"
                     /codon_start=1
                     /translation="MHMHPILLAWLVALILHAAVGSATVAMMGATAIVAPMLPLYPDI
                     SPEIIAIAIGSGAIGCTIVTDSLFWLVKQYCGATLNETFKYYTTATFIASVVALAGTF
                     LLSFII"
     CDS             558..1886
                     /note="D-serine deaminase;  (dsdA) precursor (EC
                     4.2.1.14)"
                     /codon_start=1
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPGTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFPETAATGGIIESELVAIRAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRTHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASGFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNTTHLVWATGGGMVPEEEMNQYLAKGR"
BASE COUNT      458 a    486 c    520 g    525 t
ORIGIN      143 bp upstream of HaeIII site; 51.0 min on K12 map.
        1 accctatcac tgccatgttt atcgccgtgt ttgtcgccta ttatgtgttg ggtatacggt
       61 ccagcatatg agcatgggga cgatgctcac acatacggaa aatggcttcg gttctattgc
      121 taatattttg ctgattatcg gggccggagg cgcattcaac gcattttaaa aagcagcagt
      181 ctcgctgata cgctggcagt tattctctcc aatatgcata tgcacccgat tcttctggcc
      241 tggttagtgg ctcttattct gcatgcggca gtgggctccg ctaccgtggc aatgatgggg
      301 gcaacggcaa ttgttgcacc catgctgccg ctgtatcccg acatcagccc ggaaattatt
      361 gcgattgcta tcggttcagg tgcaattggc tgcactatcg ttacggactc gcttttctgg
      421 ctagtgaagc aatattgcgg cgctacgctc aatgaaacat ttaaatacta tacgacagcg
      481 acatttatcg cttcagtcgt cgctctggcg ggcacattcc tgctgtcatt tatcatctaa
      541 gcgcaaagag acgtactatg gaaaacgcta aaatgaactc gctcatcgcc cagtatccgt
      601 tggtaaagga tctggttgct cttaaagaaa ccacctggtt taatcctggc acgacctcat
      661 tggctgaagg tttaccttat gttggcctga ccgaacagga tgttcaggac gcccatgcgc
      721 gcttatcccg ttttgcaccc tatctggcaa aagcatttcc tgaaactgct gccactgggg
      781 ggattattga atcagaactg gttgccattc gagctatgca aaaacggctg gaaaaagaat
      841 atcagcaacc gatcagcggg caactgttac tgaaaaaaga tagccatttg cccatttccg
      901 gctccataaa agcacgcggc gggatttatg aagtcctggc acacgcagaa aaactggctc
      961 tggaagcggg gttgctgacg cttgatgatg actacagcaa actgctttct ccggagttta
     1021 aacagttctt tagccaatac agcattgctg tgggctcaac cggaaatctg gggttatcaa
     1081 tcggcattat gagcgcccgc attggcttta aggtgacagt tcatatgtct gctgatgccc
     1141 gggcatggaa aaaagcgaaa ctgcgcacgc atggcgttac ggtcgtggaa tatgagcaag
     1201 attatggtgt tgccgtcgag gaaggacgta aagcagcgca gtctgacccg aactgtttct
     1261 ttattgatga cgaaaattcc cgcacgttgt tccttgggta ttccgtcgct ggccagcgtc
     1321 ttaaagcgca atttgcccag caaggccgta tcgtcgatgc tgataaccct ctgtttgtct
     1381 atctgccgtg tggtgttggc ggtggtcctg gtggcgtcgc attcgggctt aaactggcgt
     1441 ttggcgatca tgttcactgc ttttttgccg aaccaacgca ctccccttgt atgttgttag
     1501 gcgtccatac aggattacac gatcagattt ctgttcagga tattggtatc gacaacctta
     1561 ccgcagcgga tggccttgca gttggtcgcg catcaggctt tgtcgggcgg gcaatggagc
     1621 gtctgctgga tggcttctat acccttagcg atcaaaccat gtatgacatg cttggctggc
     1681 tggcgcagga agaaggtatt cgtcttgaac cttcggcact ggcgggtatg gccggacctc
     1741 agcgcgtgtg tgcatcagta agttaccaac agatgcacgg tttcagcgca gaacaactgc
     1801 gtaataccac tcatctggtg tgggcgacgg gaggtggaat ggtgccggaa gaagagatga
     1861 atcaatatct ggcaaaaggc cgttaataac gtttcaacgc agcatcgcaa tcctttccct
     1921 gggtgagcga tgctgccgat ggcgcagact taagatcccc ggtcttaccc gctataaccc
     1981 cctcctttt
//