From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Bos taurus endothelial nitric oxide synthase mRNA, complete cds.
Date: 21 Jul 92 10:03:55 GMT
Distribution: bionet
Checksum: 41739     8

LOCUS       BOVECNOS     3738 bp ss-mRNA            MAM       21-JUL-1992
DEFINITION  Bos taurus endothelial nitric oxide synthase mRNA, complete cds.
ACCESSION   M89952
KEYWORDS    guanylate cyclase; nitric oxide synthase.
SOURCE      Bos taurus aortic endothelial cDNA to mRNA.
  ORGANISM  Bos taurus
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Artiodactyla; Ruminantia; Pecora; Bovidae; Bovinae;
            Bovini.
REFERENCE   1  (bases 1 to 3738)
  AUTHORS   Lamas,S., Marsden,P.A., Li,G.K., Tempst,P. and Michel,T.
  TITLE     Endothelial nitric oxide synthase: Molecular cloning and
            characterization of a distinct constitutive enzyme isoform
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 89, 6348-6352 (1992)
  STANDARD  full staff_entry
FEATURES             Location/Qualifiers
     CDS             20..3637
                     /product="nitric oxide synthase"
                     /codon_start=1
                     /translation="MGNLKSVGQEPGPPCGLGLGLGLGLCGKQGPASPAPEPSRAPAP
                     ATPHAPDHSPAPNSPTLTRPPEGPKFPRVKNWELGSITYDTLCAQSQQDGPCTPRCCL
                     GSLVLPRKLQTRPSPGPPPAEQLLSQARDFINQYYSSIKRSGSQAHEERLQEVEAEVA
                     STGTYHLRESELVFGAKQAWRNAPRCVGRIQWGKLQVFDARDCSSAQEMFTYICNHIK
                     YATNRGNLRSAITVFPQRAPGRGDFRIWNSQLVRYAGYRQQDGSVRGDPANVEITELC
                     IQHGWTPGNGRFDVLPLLLQAPDEAPELFVLPPELVLEVPLEHPTLEWFAALGLRWYA
                     LPAVSNMLLEIGGLEFSAAPFSGWYMSTEIGTRNLCDPHRYNILEDVAVCMDLDTRTT
                     SSLWKDKAAVEINLAVLHSFQLAKVTIVDHHAATVSFMKHLDNEQKARGGCPADWAWI
                     VPPISGSLTPVFHQEMVNYILSPAFRYQPDPWKGSATKGAGITRKKTFKEVANAVKIS
                     ASLMGTLMAKRVKATILYASETGRAQSYAQQLGRLFRKAFDPRVLCMDEYDVVSLEHE
                     ALVLVVTSTFGNGDPPENGESFAAALMEMSGPYNSSPRPEQHKSYKIRFNSVSCSDPL
                     VSSWRRKRKESSNTDSAGALGTLRFCVFGLGSRAYPHFCAFARAVDTRLEELGGERLL
                     QLGQGDELCGQEEAFRGWAKAAFQASCETFCVGEEAKAAAQDIFSPKRSWKRQRYRLS
                     TQAEGLQLLPGLIHVHRRKMFQATVLSVENLQSSKSTRATILVRLDTAGQEGLQYQPG
                     DHIGICPPNRPGLVEALLSRVEDPPPPTESVAVEQLEKGSPGGPPPSWVRDPRLPPCT
                     LRQALTFFLDITSPPSPRLLRLLSTLAEEPSEQQELETLSQDPRRYEEWKWFRCPTLL
                     EVLEQFPSVALPAPLLLTQLPLLQPRYYSVSSAPNAHPGEVHLTVAVLAYRTQDGLGP
                     LHYGVCSTWLSQLKTGDPVPCFIRGAPSFRLPPDPYVPCILVGPGTGIAPFRGFWQER
                     LHDIESKGLQPAPMTLVFGCRCSQLDHLYRDEVQDAQERGVFGRVLTAFSREPDSPKT
                     YVQDILRTELAAEVHRVLCLERGHMFVCGDVTMATSVLQTVQRILATEGDMELDEAGD
                     VIGVLRDQQRYHEDIFGLTLRTQEVTSRIRTQSFSLQERHLRGAVPWAFDPPGPDTPG
                     P"
BASE COUNT      662 a   1306 c   1176 g    594 t
ORIGIN
        1 ggcacgaggc cccagcgaca tgggcaactt gaagagtgtg ggccaggagc ccgggccccc
       61 ctgcggcctg gggctggggc tgggcctcgg gctatgcggc aagcagggcc cagcctcccc
      121 ggcacctgag cccagccggg cccccgcacc cgccaccccg cacgcgccag accacagccc
      181 agctcccaac agccccacgc tgacccggcc tccggagggg cccaagttcc ctcgcgtgaa
      241 gaactgggag ctggggagca tcacctacga cactctgtgc gcgcagtccc aacaggacgg
      301 gccctgcact cccaggtgct gcctgggctc cctggtgttg ccccggaaac tgcagacccg
      361 gccctccccg ggacctccac ccgctgagca gctgctgagc caggccaggg acttcatcaa
      421 ccagtactac agctccatca agaggagtgg ctcccaggct cacgaggagc ggcttcagga
      481 ggtggaggcc gaggtggcat ccacgggcac ctaccacctc cgagagagcg agctggtgtt
      541 cggggccaag caggcctggc gcaacgcacc ccgctgcgtg ggccgcatcc agtgggggaa
      601 gctgcaggtg tttgatgccc gggactgcag ctcagcacag gagatgttca cctacatctg
      661 caaccacatc aagtacgcca ccaaccgcgg caaccttcgc tcggccatca cagtgttccc
      721 gcagcgcgcc ccgggccgcg gagacttccg gatctggaac agccagctgg tgcgctacgc
      781 aggctacaga cagcaggatg gctctgtgcg tggggaccca gccaatgtgg agatcacgga
      841 gctctgcatc cagcacggct ggacccccgg aaacggccgc ttcgacgtgc tgcccctgct
      901 gctccaggcc ccagacgagg ctccagagct ctttgttctg ccccccgagc tggtccttga
      961 agtgcccctg gagcacccca cactggagtg gttcgcggcc ctgggcctgc gatggtatgc
     1021 cctcccggcc gtgtccaaca tgctgctgga aatcgggggt ctggagttct ccgcggcccc
     1081 cttcagcggc tggtacatga gcacggagat tggcacgcgg aacctgtgtg accctcaccg
     1141 ctacaatatc ctggaggatg tggccgtctg catggacctc gacacgcgga ccacctcgtc
     1201 cctgtggaag gacaaggcgg ccgtggagat caacctggct gtgctgcaca gctttcagct
     1261 cgccaaggtg accatcgtgg accaccacgc cgccacggtg tccttcatga agcacctgga
     1321 caacgagcag aaggccaggg ggggctgccc cgccgactgg gcctggatcg tgccccccat
     1381 ctcaggcagc ctcacgcccg tcttccacca ggagatggtc aactacatcc tgtcccctgc
     1441 cttccgctac cagccagacc cctggaaagg gagcgcgacc aagggcgcag gcatcaccag
     1501 gaagaagacc tttaaggaag tggccaacgc ggtgaagatc tctgcctcac tcatgggcac
     1561 cctgatggcc aagcgagtga aagcaaccat cctgtacgcc tctgagaccg gccgggccca
     1621 gagctacgct cagcagctgg ggaggctctt ccggaaggcc ttcgatcccc gggtcctgtg
     1681 catggatgag tatgacgtgg tgtccctgga gcacgaggcg ctggtactgg tggtgaccag
     1741 cacctttggg aatggcgatc ccccggagaa tggagagagt tttgcagctg ccctgatgga
     1801 gatgtcgggg ccctacaaca gctccccgcg gccggaacag cacaagagtt acaagatccg
     1861 cttcaacagc gtctcctgct cagacccgct ggtgtcctcc tggcggcgga agagaaagga
     1921 gtccagcaac acagacagtg cgggggccct ggggaccctc aggttctgtg tgttcggact
     1981 gggctcccgg gcataccccc acttctgcgc cttcgcgcga gcggtggaca cccggctgga
     2041 agagcttgga ggggagcggc tgctgcagct gggccagggc gatgagctct gcggccagga
     2101 agaggccttc cgtggttggg caaaggcggc cttccaggcc tcctgtgaga ccttctgcgt
     2161 tggggaggag gccaaggccg ccgcccagga catcttcagc cccaaacgga gctggaaacg
     2221 ccagaggtac cggctgagca cccaggccga gggcctccag ctgctgccag gcctgatcca
     2281 cgtgcacaga cggaagatgt ttcaggccac agtcctctcg gtggaaaatc tgcaaagcag
     2341 caagtccacc cgggccacca tcctggtgcg cctggacact gcaggccagg aggggctgca
     2401 gtaccagccg ggggaccaca taggcatctg cccgcccaac cggccgggcc tggtggaggc
     2461 gctgctgagc cgcgtggagg acccgccacc gcccaccgag tctgtggctg tggagcagct
     2521 ggagaaaggc agcccaggcg gccctcctcc cagctgggtg cgggacccac ggctgccccc
     2581 gtgcacgctg cgccaggctc tcaccttctt cctggacatc acctccccac ccagcccccg
     2641 gcttctccga ctgctcagca ccctggccga agaacccagc gagcagcagg agcttgagac
     2701 cctcagtcag gacccccggc gctacgagga gtggaagtgg ttccgctgcc ccacgctgct
     2761 ggaggtgctg gagcagttcc cgtccgtggc gctgcccgcc ccgctgctcc tcacccagct
     2821 gcccctgctg cagccccggt actactctgt cagctcggcc cccaacgccc accccggaga
     2881 ggtccacctc acagtggccg tgctggcgta caggacccaa gatgggctgg gccccctaca
     2941 ctacggggtc tgctccacat ggctgagcca gctcaagact ggagaccccg tgccctgctt
     3001 catcaggggg gctccctcct tccggctgcc gcctgacccc tacgtgccct gcatcctcgt
     3061 gggccctggc actggcatcg cccccttccg gggattttgg caggagaggc tgcatgacat
     3121 tgagagcaaa gggctgcagc ccgcccccat gaccctggtg ttcggctgcc gctgctccca
     3181 actcgaccat ctctaccgcg acgaggtgca ggacgcccag gagcgcgggg tgtttggccg
     3241 cgtcctcacc gccttctccc gggaacctga cagccccaag acctacgtac aggacatcct
     3301 gagaaccgag ctggctgccg aggtgcaccg cgtgctgtgc ctcgagcggg gccacatgtt
     3361 tgtctgcggc gatgtcacta tggcaaccag cgtcctgcag acggtgcagc gcatcttggc
     3421 gacagagggc gacatggagc tggacgaggc gggcgacgtc atcggcgtgc tgcgggatca
     3481 gcaacgctat cacgaggaca ttttcggcct cacgctgcgc acccaggagg tgacaagccg
     3541 tatacgtacc cagagctttt ccctgcagga gcggcatctg cggggcgcgg tgccctgggc
     3601 cttcgacccg cccggcccag acacccccgg cccctgagac ccctcttgct tcccactgca
     3661 gttcccggag agaggggctg tcattccact atggctctac cgctgtcctg ttggccttta
     3721 ccgggaccgg ctcgtgcc
//