From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Newcastle disease virus (NDV) matrix protein (M) gene, complete
Date: 9 Apr 92 10:04:31 GMT
Distribution: bionet
Checksum: 28639     4

LOCUS       NDVMA        1210 bp ss-mRNA            VRL       09-APR-1992
DEFINITION  Newcastle disease virus (NDV) matrix protein (M) gene, complete
            cds.
ACCESSION   M16622
KEYWORDS    matrix protein; viral particle budding organization.
SOURCE      Newcastle disease virus (Australia-Victoria strain) from infected
            Chinese hamster ovary cells, cDNA to mRNA, pTL526.
  ORGANISM  Newcastle disease virus
            Viridae; ss-RNA enveloped viruses; Negative strand RNA viruses;
            Paramyxoviridae; Paramyxovirus.
REFERENCE   1  (bases 1 to 1210)
  AUTHORS   McGinnes,L.W. and Morrison,T.G.
  TITLE     The nucleotide sequence of the gene encoding the Newcastle disease
            virus membrane protein and comparisons of membrane protein
            sequences
  JOURNAL   Virology 156, 221-228 (1987)
  STANDARD  full staff_entry
REFERENCE   2  (sites)
  AUTHORS   Wang,C., Raghu,G., Morrison,T. and Peeples,M.E.
  TITLE     Intracellular processing of the Paramyxovirus F protein: Critical
            role of the predicted amphipathic alpha helix adjacent to the
            fusion domain
  JOURNAL   Unpublished (1991)
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     mRNA            1..>1210
                     /gene="M"
     CDS             35..1129
                     /product="matrix protein"
                     /gene="M"
                     /codon_start=1
                     /translation="MDSSRTIGLYFDSALPSSNLLAFPIVLQDIGDGKKQIAPQYRIQ
                     RLDSWTDSKEDSVFITTYGFIFQVGNEEVTVGMISDNPKHELLSAAMLCLGSVPNVGD
                     LVELARACLTMVVTCKKSATDTERMVFSVVQAPQVLQSCRVVANKYSSVNAVKHVKAP
                     EKIPGSGTLEYKVNFVSLTVVPRKDVYKIPTAALKVSGSSLYNLALNVTIDVEVDPKS
                     PLVKSLSKSDSGYYANLFLHIGLMSTVDKKGKKVTFDKLERKIRRLDLSVGLSDVLGP
                     SVLVKARGARTRLLAPFFSSSGTACYPISNASPQVAKILWSQTARLRSVKVIIQAGTQ
                     RAVAVTADHEVTSTKIEKRHTIAKYNPFKK"
     mutation        249
                     /note="replace (249,'g')"
                     /note="ts mutant D1 and revertant D1R3 derived from D1"
                     /evidence=EXPERIMENTAL
                     /citation=[2]
BASE COUNT      330 a    284 c    302 g    294 t
ORIGIN      108 bp upstream of PvuI site.
        1 acgggtagaa tcggagtgcc ctgattgtgc caagatggac tcatctagga caatagggct
       61 atactttgat tctgcccttc cttctagcaa cctgttagca ttcccgatcg tcctacaaga
      121 cataggagat gggaagaagc aaatcgcccc gcaatatagg atccagcgtc ttgactcgtg
      181 gacagacagt aaagaagact cggtattcat caccacctat ggattcatct tccaggttgg
      241 gaatgaagaa gtcactgtcg gcatgatcag cgataatccc aagcacgagt tactttcagc
      301 tgcgatgctc tgcctaggaa gtgtcccgaa tgtcggagat cttgttgagt tggcaagggc
      361 ctgcctcact atggtggtaa catgcaagaa gagtgcaact gatactgaga gaatggtctt
      421 ctcggtagta caggcgcccc aggtgctgca aagctgtagg gtcgtggcaa acaaatactc
      481 gtcagtgaat gcagttaagc acgtgaaagc accagagaag atccctggga gcggaaccct
      541 agagtacaag gtgaattttg tctctttgac tgtggtgcca aggaaggatg tctacaaaat
      601 cccaaccgca gcattgaagg tatctggctc gagcctgtac aatcttgcgc tcaatgtcac
      661 tattgatgtg gaggtagacc caaagagccc gttagtcaaa tctctttcaa agtccgacag
      721 tggatactat gctaatcttt tcttacatat cggacttatg tccactgtag ataagaaggg
      781 aaagaaagtg acatttgaca agctggagag gaagataaga agactcgatt tatctgtcgg
      841 gctcagtgat gtgctcggac cttccgtgct tgtgaaggcg agaggtgcac ggactaggct
      901 gctggcacct ttcttctcta gcagtgggac agcctgctat cctatatcaa atgcctctcc
      961 tcaggtagct aagatactct ggagtcaaac tgcgcgcctg cggagtgtaa aagtcattat
     1021 tcaagcgggc acccaacgcg ctgtcgcggt gaccgctgac cacgaggtca cctctactaa
     1081 gatagaaaag aggcatacca ttgctaaata caatcctttt aagaaataag ctgcatctct
     1141 gagactgcaa tccgcccgct ttcccgaatc atcacgacgc ttaataatgg atctgtcctg
     1201 attactcaca
//