From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: B.napus mRNA for myrosinase
Date: 25 Jul 92 10:02:42 GMT
Distribution: bionet
Checksum: 08130     5

LOCUS       BNAMYRO      1804 bp ss-RNA             PLN       25-JUL-1992
DEFINITION  B.napus mRNA for myrosinase
ACCESSION   X60214
KEYWORDS    myrosinase.
SOURCE      Brassica napus RNA.
  ORGANISM  Brassica napus
            Eukaryota; Plantae; Embryobionta; Magnoliophyta; Magnoliopsida;
            Dilleniidae; Capparales; Brassicaceae.
REFERENCE   1  (bases 1 to 1804)
  AUTHORS   Falk,A., Xue,J., Lenman,M. and Rask,L.
  TITLE     Sequence of a cDNA clone encoding the enzyme myrosinase, and
            expression of myrosinase in different tissues of Brassica napus
  JOURNAL   Plant Sci. 83, 181-186 (1992)
  STANDARD  full automatic
COMMENT     *source: strain=Swalfs Karat; *source: clone_library=plasmid;
            *source: clone=Myr1;
            From EMBL    entry BNMYRO;  dated 26-JUN-1992.
FEATURES             Location/Qualifiers
     CDS             8..1654
                     /product="myrosinase"
                     /codon_start=1
                     /translation="MKLLHGLALVFLLAAASCKADEEITCEENNPFTCSNTDILSSKN
                     FGKDFIFGVASSAYQIEGGRGRGVNVWDGFSHRYPEKAGSDLKNGDTTCESYTRWQKD
                     VDVMGELNATGYRFSFAWSRIIPKGKVSRGVNQGGLDYYHKLIDALLEKNITPFVTLF
                     HWDLPQTLQDEYEGFLDRQIIQDFKDYADLCFKEFGGKVKHWITINQLYTVPTRGYAI
                     GTDAPGRCSPMVDTKHRCYGGNSSTEPYIVAHNQLLAHATVVDLYRTKYKFQKGKIGP
                     VMITRWFLPFDESDPASIEAAERMNQFFHGWYMEPLTKGRYPDIMRQIVGSRLPNFTE
                     EEAELVAGSYDFLGLNYYVTQYAQPKPNPYPSETHTAMMDAGVKLTYDNSRGEFLGPL
                     FVEDKVNGNSYYYPKGIYYVMDYFKTKYGDPLIYVTENGFSTPSSENREQAIADYKRI
                     DYLCSHLCFLRKVIKEKGVNVRGYFAWALGDNYEFCKGFTVRFGLSYVNWEDLDDRNL
                     KESGKWYQRFINGTVKNAVKQDFLRSSLSSQSQKKRFADA"
     polyA_signal    1751..1756
     polyA_signal    1764..1769
BASE COUNT      559 a    390 c    387 g    468 t
ORIGIN
        1 attaaacatg aagcttcttc atggactcgc tttagttttt ctattagctg ctgcgagttg
       61 caaagctgat gaagaaatta cttgcgaaga gaacaaccca ttcacatgta gtaacactga
      121 tattttaagc agtaagaact tcggaaaaga tttcatcttc ggtgttgcat cttctgctta
      181 ccagattgaa ggagggagag gtcgtggtgt taacgtttgg gatggcttca gtcaccgata
      241 cccagagaaa gctgggtcag acttgaagaa tggagacact acttgtgagt catatacgag
      301 atggcagaaa gatgtagacg tgatgggcga actcaatgct actggctaca ggttctcctt
      361 tgcgtggtca agaatcattc caaaaggaaa ggtgagtagg ggagtgaacc aaggaggtct
      421 tgattactac cacaaactca tagatgcact cctcgaaaag aatataacgc ctttcgttac
      481 cctctttcac tgggaccttc ctcaaacact ccaagatgag tatgaaggtt tcttagaccg
      541 ccagatcata caagatttca aagactacgc ggatctatgt ttcaaagaat ttggtggaaa
      601 ggtaaagcac tggatcacga tcaaccagct atacacagtg cctacaagag gctatgctat
      661 cggaacagat gcaccaggtc gatgttctcc tatggttgat accaagcaca ggtgttacgg
      721 tggaaattct tcaacagaac cctacatcgt tgcacataac cagcttcttg ctcatgccac
      781 ggtcgtcgat ctttacagga ccaaatacaa gttccaaaaa gggaagattg gacctgtaat
      841 gattacaaga tggtttcttc catttgatga gtctgatcct gcctccatag aagcagctga
      901 gaggatgaac caattcttcc atggatggta catggagccg ctaacaaagg gtagataccc
      961 agacatcatg aggcagattg tgggtagtag gcttcccaac ttcaccgagg aagaagcaga
     1021 actcgttgct ggttcatatg attttcttgg tctaaactat tacgtcaccc aatacgccca
     1081 gccaaaacct aacccatatc cttcagagac acacactgcc atgatggacg ctggcgtaaa
     1141 gctcacatat gataattcac gtggtgaatt tcttggtcca ctgtttgttg aagacaaagt
     1201 aaacggcaac agctattact acccaaaagg catttattac gttatggact acttcaaaac
     1261 caaatacggc gaccctttaa tctatgtcac cgaaaatgga tttagtaccc ccagttcaga
     1321 aaaccgtgag caagctattg ccgactacaa gcgaatcgat tatctatgca gccatctatg
     1381 ttttctccgc aaggtgatca aggagaaggg tgtcaacgtg agaggatact ttgcatgggc
     1441 tcttggagat aattatgaat tctgtaaagg tttcaccgtc agatttggac tcagttacgt
     1501 taattgggaa gatcttgacg atagaaacct caaagaatct ggcaaatggt accagagatt
     1561 catcaacggg accgtcaaga acgctgtgaa acaagatttc ctccgctcaa gcctctcttc
     1621 ccagagtcag aagaagaggt tcgctgatgc atgaaacact tttccccccg gtcaagatca
     1681 tctccatgtc tctcactctt tctagttgct ccaaagataa ggagcttctt ctacctacaa
     1741 taatgtacta aataaatatt ctcaataaaa agatgatcag ttactaatga aaaaaaaaaa
     1801 aaaa
//