From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Lactobacillus sake catalase (katA) gene, complete cds.
Date: 1 Sep 92 10:02:50 GMT
Distribution: bionet
Checksum: 05376     5

LOCUS       LBAKATA      1779 bp ds-DNA             BCT       01-SEP-1992
DEFINITION  Lactobacillus sake catalase (katA) gene, complete cds.
ACCESSION   M84015
KEYWORDS    catalase; katA gene.
SOURCE      Lactobacillus sake (strain LTH677) DNA.
  ORGANISM  Lactobacillus sake
            Prokaryotae; Firmicutes; Regular asporogenous rods;
            Lactobacillaceae.
REFERENCE   1  (bases 1 to 1779)
  AUTHORS   Knauf,H.J., Vogel,R.F. and Hammes,W.P.
  TITLE     Cloning, sequence and phenotypic expression of katA, which encodes
            the catalase of Lactobacillus sake LTH677
  JOURNAL   Appl. Environ. Microbiol. 58, 832-839 (1992)
  STANDARD  simple staff_entry
FEATURES             Location/Qualifiers
     -35_signal      86..91
                     /gene="katA"
     -10_signal      109..115
                     /gene="katA"
     RBS             172..177
                     /standard_name="Shine Dalgarno sequence"
                     /gene="katA"
     CDS             187..1623
                     /EC_number="1.11.1.6"
                     /product="catalase"
                     /gene="katA"
                     /evidence=EXPERIMENTAL
                     /codon_start=1
                     /translation="MTNQLTTNEGQPWADNQHSQTAANAAPSLIQDYQLLEKLAHFNR
                     ERIPERVVHAKGAGRKGYFKVTKDMSAYTKAAVFSGVGKKTPLITRFSQVAGEAGYPD
                     TYRDVRGFAVKFYTEEGNYDIVGNNTPVFFVNDPLKFPDFIHSQKRDPRTHARSQDMQ
                     WDFWSLSPESVHQVTILMSDRGIPASYRMMHGFGSHTFKWVNAQGEQFWVIFHFKTNQ
                     GIHNLSNELADELAGKDTDYLQNDLFDAIETGDYPSWTVAVQLVLMKMAELSPKDIFD
                     VTKVISQKDYPLIEIGQMVLDENPTNNFEDIQELAFSPANLVPGIEASPDKLLQGRLF
                     GYKDAERYRLGANYEQLPVNRPKVPVHNYERDGAMAQNQATGVNYEPNSQDGPTEVPA
                     AKIHGDQLSGTTGNFSADPDYYSAAGKLYRLLSADEQTRLIENIRMNLGQVTKPEIQI
                     REVKQFYQADPEYGRRVATSVKLRFSSV"
     terminator      1638..1682
                     /standard_name="rho-independent termination sequence"
                     /gene="katA"
BASE COUNT      505 a    448 c    357 g    469 t
ORIGIN
        1 gcgtgtctaa ataccactaa ccccgaaaaa gaactgccga taacctcggc agttcttttt
       61 agtaacttgt tgagcaagct cttcattgac ggtgcctgtt gaaggtccta tagtgaccta
      121 ggtaagttgc gcaccatctt ttcagtgtgt tccatgtttt taactattct taggaggtca
      181 aatattatga caaatcaact aacgactaac gaggggcaac cgtgggcgga caatcaacat
      241 tcgcaaactg ccgccaacgc ggccccgtcc ttaatccaag attatcaatt actcgaaaaa
      301 ctcgcccact ttaaccgcga acgcatccct gaacgggtgg tgcatgccaa aggcgctggc
      361 cgtaaaggct atttcaaggt taccaaggac atgagcgcat ataccaaagc cgctgttttc
      421 agtggcgtcg gcaaaaaaac accgcttatc actcgttttt ctcaagtcgc tggtgaagcc
      481 ggctatcccg atacataccg cgatgtccgc ggtttcgccg ttaaattcta tacggaagaa
      541 ggcaattacg atattgtcgg caataacacg ccggtcttct tcgtcaatga tccactaaaa
      601 ttccccgatt tcatccactc tcaaaaacgt gatccccgga cacatgcccg tagccaagat
      661 atgcaatggg atttctggtc cctgtcaccc gaatctgtcc accaagtcac gattctcatg
      721 agtgatcgcg ggattcctgc tagttaccgg atgatgcacg gctttggtag ccacaccttc
      781 aaatgggtta acgcacaagg tgaacaattc tgggttatat tccatttcaa gacgaaccaa
      841 ggtattcaca atctcagcaa cgaactcgcc gatgaactcg ctggtaagga tactgattac
      901 cttcaaaatg atttattcga cgcaattgaa accggcgatt atccaagttg gacggttgcc
      961 gtccaactcg tccttatgaa gatggctgaa ttatctccca aggatatttt tgatgttact
     1021 aaggttattt cacaaaagga ttatccatta atcgaaatcg gtcaaatggt cctcgatgaa
     1081 aatccaacga ataacttcga agatatccaa gaactggcct tctcaccggc taacttagtc
     1141 cctgggattg aagcatcacc cgacaaatta cttcaaggtc gactatttgg ctataaggat
     1201 gctgaacgtt accggcttgg tgccaactac gagcaactcc ctgtcaaccg accaaaagtc
     1261 cccgttcata attacgaacg tgacggtgcg atggcccaaa atcaagcaac tggcgttaac
     1321 tacgaaccca acagtcaaga tggacccact gaagtcccag cagctaagat tcatggcgat
     1381 caactctctg gtacaactgg caacttctct gccgatcccg attattactc agcagctggc
     1441 aaactttacc ggttattatc agccgatgaa caaacccgct taatcgaaaa tattcgcatg
     1501 aatcttggtc aagtgactaa accagaaatt caaattcgcg aagttaaaca attttaccaa
     1561 gctgatccag aatatggtcg gcgcgtcgca accagcgtta aacttagatt tagctcagtt
     1621 tgaataatca ctaacacgaa aaaataggtg gccccagttt gggacaccta ttttttattc
     1681 gttatctttt ttccctgtct cttgttttgc cgccactctt ttttcggtta ataatcagaa
     1741 acgtcatctt gattcgtaac gttaagcagt caattatgc
//