From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Nicotiana sylvestris (clone 6PU4) pentameric polyubiquitin (ubi4)
Date: 31 Dec 92 01:41:17 GMT
Distribution: bionet
Checksum: 16025 5

LOCUS       TOBUBI4A     1321 bp ss-mRNA            PLN       17-DEC-1992
DEFINITION  Nicotiana sylvestris (clone 6PU4) pentameric polyubiquitin (ubi4)
            mRNA, complete cds.
ACCESSION   M74156
KEYWORDS    polyprotein; protein degradation; stress protein; ubiquitin.
SOURCE      Nicotiana sylvestris (library: lambda gt10) protoplast cDNA to
            mRNA.
  ORGANISM  Nicotiana sylvestris
            Eukaryota; Plantae; Embryobionta; Magnoliophyta; Magnoliopsida;
            Asteridae; Solanales; Solanaceae.
REFERENCE   1  (bases 1 to 1321)
  AUTHORS   Genschik,P., Parmentier,Y., Durr,A., Marbach,J., Criqui,M.C.,
            Jamet,E. and Fleck,J.
  TITLE     Ubiquitin genes are differentially regulated in protoplast-derived
            cultures of Nicotiana sylvestris and in response to various
            stresses
  JOURNAL   Plant Mol. Biol. 20, 897-910 (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     mat_peptide     <1..217
                     /product="ubiquitin monomer"
                     /gene="ubi4"
                     /codon_start=2
     mat_peptide     218..445
                     /product="ubiquitin monomer"
                     /gene="ubi4"
                     /codon_start=1
     mat_peptide     446..673
                     /product="ubiquitin monomer"
                     /gene="ubi4"
                     /codon_start=1
     mat_peptide     674..901
                     /product="ubiquitin monomer"
                     /gene="ubi4"
                     /codon_start=1
     mat_peptide     902..1129
                     /product="ubiquitin monomer"
                     /gene="ubi4"
                     /codon_start=1
     3'UTR           1136..1321
                     /gene="ubi4"
                     /evidence="EXPERIMENTAL"
     polyA_signal    1299..1304
                     /gene="ubi4"
     polyA_site      1321
                     /gene="ubi4"
                     /evidence="EXPERIMENTAL"
     CDS             <1..1135
                     /gene="ubi4"
                     /evidence="EXPERIMENTAL"
                     /product="pentameric polyubiquitin"
                     /codon_start=2
                     /translation="VKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGK
                     QLEDGRTLADYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVESSDTIDNVKAKI
                     QDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGGMQIFVKTLTGKT
                     ITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLV
                     LRLRGGMQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLED
                     GRTLADYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKE
                     GIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGGF"
BASE COUNT      368 a    285 c    333 g    335 t
ORIGIN      
        1 ggtgaaaacc ctaaccggaa aaacaatcac ccttgaggtt gaaagctccg acactattga
       61 caacgttaag gctaagattc aggacaaaga aggaatccca ccggaccagc agaggttgat
      121 cttcgccgga aagcagctcg aagacggtag aaccctagcc gactacaaca tccagaagga
      181 atcgaccctt cacttggtgc tccgtcttcg tggcggtatg caaatctttg ttaaaaccct
      241 aaccgggaaa acaatcaccc ttgaagtcga aagctccgac actatcgaca acgttaaggc
      301 taagattcag gacaaggagg gaatcccacc agaccagcaa aggttgattt tcgccggaaa
      361 gcaactcgaa gacggcagaa ccctagctga ttacaacatc cagaaggaat cgacccttca
      421 cttggtcctt cgtcttcgtg gtgggatgca gattttcgtc aaaaccttaa ctgggaaaac
      481 aatcaccctt gaagtcgaaa gctccgacac tattgacaat gttaaggcta aaatccagga
      541 taaggaggga atcccaccag accagcaaag gttgattttt gctggtaagc aattggaaga
      601 tggaaggacc ttagctgatt acaatattca gaaggagtcg actcttcact tggtgctccg
      661 tcttcgtggt ggaatgcaga tttttgtcaa gactttgacc gggaaaacca tcactcttga
      721 ggtggaaagc tctgacacta tcgacaacgt taaggcgaag atccaggata aggagggtat
      781 cccaccagat cagcaaaggc tgatctttgc tggtaagcag ttggaagatg gccgaaccct
      841 tgctgattac aacatccaga aggagtcaac tttgcacctt gtgcttcgtc ttcgtggtgg
      901 gatgcagatt tttgtgaaga cgttgacagg gaaaaccatc actttggagg tggagagttc
      961 cgatactatt gacaatgtga aggctaagat tcaggacaag gagggtattc ccccggatca
     1021 gcagaggctg atttttgctg ggaagcagtt ggaagatgga agaactctgg cggattataa
     1081 catccagaag gagtcgactc tgcaccttgt cctccgtctc cgtggtggtt tctaaactgt
     1141 ccgtcggtag tggtggcaat gcctgtgtct gtgtcttggg tctgtttgct gtttgtttga
     1201 ttcatgattt agtagtttgt gtagtttctg ttacttgtca tcatgtcatg tctccaaaag
     1261 aggcaaggag acttgttctc ttgtctctgt ttgtgaataa taaagtccga attatggttt
     1321 a
//