From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: H.sapiens GNS mRNA encoding glucosamine-6-sulphatase.
Date: 21 Jul 92 10:05:27 GMT
Distribution: bionet
Checksum: 50757     7

LOCUS       HUMGNSM      2379 bp ss-RNA             PRI       21-JUL-1992
DEFINITION  H.sapiens GNS mRNA encoding glucosamine-6-sulphatase.
ACCESSION   Z12173
KEYWORDS    GNS gene; N-acetylglucosamine-6-sulphatase.
SOURCE      Homo sapiens RNA.
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 618 to 2379)
  AUTHORS   Robertson,D.A., Freeman,C.C., Nelson,P.V., Morris,C.P. and
            Hopwood,J.J.
  TITLE     Human glucosamine-6-sulphatase cDNA reveals holmology with steroid
            sulphatase
  JOURNAL   Biochem. Biophys. Res. Commun. 157, 218-224 (1988)
  STANDARD  full automatic
REFERENCE   2  (bases 1 to 2379)
  AUTHORS   Morris,C.A.
  JOURNAL   Unpublished (1992)
  STANDARD  full staff_review
REFERENCE   3  (bases 1 to 2379)
  AUTHORS   Robertson,D.A., Freeman,C., Morris,C.A. and Hopwood,J.J.
  TITLE     A cDNA clone for human glucosamine-6-sulphatase reveals differences
            between aryl- and non-arylsulphatases
  JOURNAL   Biochem. J. (1992) In press
  STANDARD  full staff_review
COMMENT     *source: tissue=Endothelial; *source: cell_type=Fibroblast;
            *source: clone_library=Clontech lambda gt11 endothelial cDNA
            *source: library; *source: clone=p6s50, p6s41, p6s18; *source:
            chromosome=12; *source: is_macronuclear=N; *source: is_proviral=N;
            *source: is_germline=N;
            From EMBL    entry HSGNSM;  dated 09-JUN-1992.
FEATURES             Location/Qualifiers
     sig_peptide     88..195
                     /evidence=EXPERIMENTAL
                     /citation=[3]
                     /citation=[2]
     CDS             88..1746
                     /function="hydrolysis of heparan sulphate"
                     /EC_number="3.1.6.14"
                     /note="glucosamine-6-sulphatase"
                     /product="N-acetylglucosamine-6-sulphatase"
                     /gene="GNS"
                     /evidence=EXPERIMENTAL
                     /codon_start=1
                     /translation="MRLLPLAPGRLRRGSPRHLPSCSPALLLLVLGGCLGVFGVAAGT
                     RRPNVVLLLTDDQDEVLGGMTPLKKTKALIGEMGMTFSSAYVPSALCCPSRASILTGK
                     YPHNHHVVNNTLEGNCSSKSWQKIQEPNTFPAILRSMCGYQTFFAGKYLNEYGAPDAG
                     GLEHVPLGWSYWYALEKNSKYYNYTLSINGKARKHGENYSVDYLTDVLANVSLDFLDY
                     KSNFEPFFMMIATPAPHSPWTAAPQYQKAFQNVFAPRNKNFNIHGTNKHWLIRQAKTP
                     MTNSSIQFLDNAFRKRWQTLLSVDDLVEKLVKRLEFTGELNNTYIFYTSDNGYHTGQF
                     SLPIDKRQLYEFDIKVPLLVRGPGIKPNQTSKMLVANIDLGPTILDIAGYDLNKTQMD
                     GMSLLPILRGASNLTWRSDVLVEYQGEGRNVTDPTCPSLSPGVSQCFPDCVCEDAYNN
                     TYACVRTMSALWNLQYCEFDDQEVFVEVYNLTADPDQITNIAKTIDPELLGKMNYRLM
                     MLQSCSGPTCRTPGVFDPGYRFDPRLMFSNRGSVRTRRFSKHLL"
                     /citation=[3]
                     /citation=[2]
     mat_peptide     217..1743
                     /product="N-acetylglucosamine-6-sulphatase"
                     /evidence=EXPERIMENTAL
                     /citation=[3]
                     /citation=[2]
BASE COUNT      615 a    583 c    553 g    628 t
ORIGIN
        1 ggaattccgg tcggcctctc gcccttcagc tacctgtgcg tccctccgtc ccgtcccgtc
       61 ccggggtcac cccggagcct gtccgctatg cggctcctgc ctctagcccc aggtcggctc
      121 cggcggggca gcccccgcca cctgccctcc tgcagcccag cgctgctact gctggtgctg
      181 ggcggctgcc tgggggtctt cggggtggct gcgggaaccc ggaggcccaa cgtggtgctg
      241 ctcctcacgg acgaccagga cgaagtgctc ggcggcatga caccactaaa gaaaaccaaa
      301 gctctcatcg gagagatggg gatgactttt tccagtgctt atgtgccaag tgctctctgc
      361 tgccccagca gagccagtat cctgacagga aagtacccac ataatcatca cgttgtgaac
      421 aacactctgg aggggaactg cagtagtaag tcctggcaga agatccaaga accaaatact
      481 ttcccagcaa ttctcagatc aatgtgtggt tatcagacct tttttgcagg gaaatattta
      541 aatgagtacg gagccccaga tgcaggtgga ctagaacacg ttcctctggg ttggagttac
      601 tggtatgcct tggaaaagaa ttctaagtat tataattaca ccctgtctat caatgggaag
      661 gcacggaagc atggtgaaaa ctatagtgtg gactacctga cagatgtttt ggctaatgtc
      721 tccttggact ttctggacta caagtccaac tttgagccct tcttcatgat gatcgccact
      781 ccagcgcctc attcgccttg gacagctgca cctcagtacc agaaggcttt ccagaatgtc
      841 tttgcaccaa gaaacaagaa cttcaacatc catggaacga acaagcactg gttaattagg
      901 caagccaaga ctccaatgac taattcttca atacagtttt tagataatgc atttaggaaa
      961 aggtggcaaa ctctcctctc agttgatgac cttgtggaga aactggtcaa gaggctggag
     1021 ttcactgggg agctcaacaa cacttacatc ttctatacct cagacaatgg ctatcacaca
     1081 ggacagtttt ccttgccaat agacaagaga cagctgtatg agtttgatat caaagttcca
     1141 ctgttggttc gaggacctgg gatcaaacca aatcagacaa gcaagatgct ggttgccaac
     1201 attgacttgg gtcctactat tttggacatt gctggctacg acctaaataa gacacagatg
     1261 gatgggatgt ccttattgcc cattttgaga ggtgccagta acttgacctg gcgatcagat
     1321 gtcctggtgg aataccaagg agaaggccgt aacgtcactg acccaacatg cccttccctg
     1381 agtcctggcg tatctcaatg cttcccagac tgtgtatgtg aagatgctta taacaatacc
     1441 tatgcctgtg tgaggacaat gtcagcattg tggaatttgc agtattgcga gtttgatgac
     1501 caggaggtgt ttgtagaagt ctataatctg actgcagacc cagaccagat cactaacatt
     1561 gctaaaacca tagacccaga gcttttagga aagatgaact atcggttaat gatgttacag
     1621 tcctgttctg ggccaacctg tcgcactcca ggggtttttg accccggata caggtttgac
     1681 ccccgtctca tgttcagcaa tcgcggcagt gtcaggactc gaagattttc caaacatctt
     1741 ctgtagcgac ctcacacagc ctctgcagat ggatccctgc acgcctcttt ctgatgaagt
     1801 gattgtagta ggtgtctgta gctagtcttc aagaccacac ctggaagagt ttctgggctg
     1861 gctttaagtc ctgtttgaaa aagcaaccca gtcagctgac ttcctcgtgc aatgtgttaa
     1921 actgtgaact ctgcccatgt gtcaggagtg gctgtctctg gtctcttcct ttagctgaca
     1981 aggacactcc tgaggtcttt gttctcactg tatttttttt atcctggggc cacagttctt
     2041 gattattcct cttgtggtta aagactgaat ttgtaaaccc attcagataa atggcagtac
     2101 tttaggacac acacaaacac acagatacac cttttgatat gtaagcttga cctaaagtca
     2161 aaggacctgt gtagcatttc agattgagca cttcactatc aaaaatacta acatcacatg
     2221 gcttgaagag taaccatcag agctgaatca tccaagtaag aacaagtacc attgttgatt
     2281 gataagtaga gatacatttt ttatgatgtt catcacagtg tggtaaggtt gcaaattcaa
     2341 aacatgtcac ccaagctctg ttcatgtttt tgtgaattc
//