From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Equine herpesvirus type 1 (PR3 mutant) TK gene for mutant thymidine
Date: 11 Sep 92 10:04:02 GMT
Distribution: bionet
Checksum: 57972     3

LOCUS       HSE1PR3TK    1059 bp ds-DNA             VRL       11-SEP-1992
DEFINITION  Equine herpesvirus type 1 (PR3 mutant) TK gene for mutant thymidine
            kinase
ACCESSION   X67962
KEYWORDS    mutant; thymidine kinase; tk gene.
SOURCE      Equine herpesvirus type 1 DNA.
  ORGANISM  Equine herpesvirus type 1
            Viridae; ds-DNA enveloped viruses; Herpesviridae;
            Alphaherpesvirinae.
REFERENCE   1  (bases 1 to 1059)
  AUTHORS   Corrochano,L.M., Madueno,F., Field,H.J. and De la Fuente,R.
  TITLE     DNA sequence analysis of thymidine kinase defective mutants of
            equine herpesvirus-1 (EHV-1)
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
COMMENT     *source: strain=Ab4 (PR3 mutant);
            From EMBL    entry EH1PR3TK;  dated 26-AUG-1992.
FEATURES             Location/Qualifiers
     CDS             1..1059
                     /product="mutant thymidine kinase"
                     /gene="tk"
                     /codon_start=1
                     /translation="MAARVPSGEARRSASGAPVRRQVTIVRIYLDGVYGIGTSTTGRV
                     MASAASGGSPTLYFPEPMAYWRTLFEADVISGIYDTQNRKQQGDLAADDAASITAHYQ
                     SRFTTPYLILHDHTFGLFGGDSLQRGTRPDLTVVFDRHPVASAVCFPAARYLIGDMSM
                     CALIAMVATLPREPQGGNIVVTTLNVDEHVRRLRTRARIGEQIDMKLIATLRNVYSML
                     ANTSNFLRSGRVWRDGWGELPLSCETYKHRATQMDAFQERESPELSDTLFAMFKTPEL
                     LDDRGVILEVHAWALDALMLKLRNLSVFCADLSGTPRQCAATVESLIPLMSSTLSDSE
                     SASSLERAARTFNAEMGV"
     mutation        113
                     /note="replace(113,'a')"
                     /partial
BASE COUNT      223 a    307 c    308 g    221 t
ORIGIN
        1 atggcggctc gcgtaccttc cggggaagct cgacggagcg ccagcggggc gccggtcagg
       61 cggcaagtaa caatagttag aatttacctc gatggggtct acggcatcgg cacgagcacg
      121 actggacgag ttatggcatc ggctgcgagt ggaggaagtc caactctata ctttcctgag
      181 cctatggcgt actggcggac tctctttgaa gcggacgtaa ttagtggtat ttacgacacc
      241 cagaaccgga aacagcaggg agatttggcg gctgatgacg cggcgtcaat aacggcgcac
      301 taccagagcc gctttaccac gccctacctt atcctacacg atcacacatt tgggttgttt
      361 gggggtgaca gcctacagcg tgggacaaga ccagacctaa ccgtcgtttt tgaccgccac
      421 ccagtcgcct ctgccgtgtg ctttcccgcc gctcgctacc tcatcggaga catgtccatg
      481 tgcgcgctga ttgccatggt tgccacccta cccagggaac cgcaaggcgg aaacatcgtg
      541 gttaccaccc tcaatgtgga cgagcacgtg cgaagactgc gcacccgcgc cagaatcggg
      601 gaacagattg acatgaagct aatcgccaca ctgcgaaacg tgtactctat gctcgctaat
      661 actagcaact ttttgcgctc cgggagagta tggcgcgacg gctgggggga gttgcccctt
      721 tcgtgcgaga cctataaaca tcgcgcaacg cagatggacg ccttccagga gcgcgaatct
      781 cctgagctga gcgacacgtt gtttgccatg tttaagactc ccgagctgct agacgatcgt
      841 ggagtgatat tggaagttca cgcctgggcg cttgacgcgc tgatgctaaa gctgcgcaac
      901 ctgagtgttt tttgcgctga tctgagcggg actccgcgcc agtgtgctgc aaccgtggag
      961 tctctaatac ccctcatgag cagcaccctc tccgattcgg agtcggcctc ctccctggag
     1021 cgggccgcgc gcaccttcaa cgccgagatg ggcgtctga
//