From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Mouse pyruvate dehydrogenase (pdha-2) mRNA, complete cds.
Date: 30 Jul 92 10:02:10 GMT
Distribution: bionet
Checksum: 43713     5

LOCUS       MUSPDHA2A    2349 bp ss-mRNA            ROD       30-JUL-1992
DEFINITION  Mouse pyruvate dehydrogenase (pdha-2) mRNA, complete cds.
ACCESSION   M76728
KEYWORDS    lactic acidosis; mitochondrial matrix; pyruvate dehydrogenase.
SOURCE      Mus musculus (strain CD-1) testis cDNA to mRNA.
  ORGANISM  Mus musculus
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Rodentia; Myomorpha; Muridae; Murinae.
REFERENCE   1  (bases 1 to 2349)
  AUTHORS   Fitzgerald,J., Hutchison,W.M. and Dahl,H.-H.M.
  TITLE     Isolation and characterization of the mouse PDH E1a genes
  JOURNAL   Biochim. Biophys. Acta 1131, 83-90 (1992)
  STANDARD  full staff_entry
FEATURES             Location/Qualifiers
     RBS             465..475
                     /note="SP-1 binding site"
                     /gene="pdha-2"
     CDS             608..1783
                     /EC_number="1.2.4.1"
                     /product="pyruvate dehydrogenase"
                     /gene="pdha-2"
                     /codon_start=1
                     /translation="MRKMLTAVLSHVFSGMVQKPALRGLLSSLKFSNDATCDIKKCDL
                     YRLEEGPPTSTVLTRAEALKYYRTMQVIRRMELKADQLYKQKFIRGFCHLCDGQEACC
                     VGLEAGMNPTDHVITSYRAHGFCYTRGLSVKSILAELTGRKGGCAKGKGGSMHMYGKN
                     FYGGNGIVGAQVPLGAGVAFACKYLKNGQVCLALYGDGAANQGQVFEAYNMSALWKLP
                     CVFICENNLYGMGTSNERSAASTDYHKKGFIIPGLRVNGMDILCVREATKFAADHCRS
                     GKGPIVMELQTYRYHGHSMSDPGISYRSREEVHNVRSKSDPMMLLRERIISNNLSNIE
                     ELKEIDADVKKEVEDAAQFATTDPEPAVEDMANYLYHQDPPFEVRGAHKWLKYKSHS"
     misc_feature    608..697
                     /note="mitochondrial import sequence"
                     /gene="pdha-2"
     polyA_signal    1870..1879
                     /gene="pdha-2"
     polyA_signal    2123..2128
                     /gene="pdha-2"
BASE COUNT      618 a    527 c    557 g    647 t
ORIGIN
        1 ctgtattgta catactttca ataaatgtta agatgttgtt atttttttct ttaaaaacga
       61 aaaggtagaa gaaagaatga tgtcattttg ctccctccct ccttccctcc tgcctgccct
      121 tcctccttct cctcttttcc tcctctccac cgcccctcat tttgaggcca aagagaccac
      181 aaactgactg agaataggat tcaagtccgg ctcctacaaa cctcaatgcc tagtgtctga
      241 gattacaggt atgtgtggca cagttactgt ctgagtttag aggtgctggg atggaaccca
      301 aaagctctgc acagtaggct atgtccctac cctgccagga cttttttgag ttgtacaggg
      361 aaaagacact cagttgctgt gtgagtggca accatgatgt taaataggag gaaagtgtgg
      421 gagatcctcc ctttccagtt agaagcatca gaggagcagg ccccgtgggc gtggcttcca
      481 gcttgctgac gtaggcaacg cgtttgcatc ccgttattgt tgcatcagag gagctcggca
      541 gccatcttaa aagccactga gtgatcgttg ggagccgagc cgctaccgtt gtgcctcgcg
      601 tttctccatg aggaaaatgc tgaccgctgt gctgtctcac gtattttcgg gaatggtcca
      661 aaagccagct ctcagaggac tgctgtcatc tctgaagttc tccaacgacg ccacctgtga
      721 cattaagaaa tgtgacctgt accggctgga ggagggccca ccgacctcca ccgtgctcac
      781 ccgagccgag gccctcaagt actaccggac catgcaggta attcggcgca tggagttgaa
      841 ggccgaccag ctgtataagc agaaattcat ccgtggtttc tgtcacctgt gtgatgggca
      901 ggaagcctgc tgcgtggggc tggaggcagg gataaatccc acggatcacg tcatcacgtc
      961 ctaccgggct catggcttct gctacacgcg aggactgtcc gtgaagtcca ttctcgccga
     1021 gctgactgga cgcaaaggag gctgtgctaa aggcaaggga ggctccatgc acatgtacgg
     1081 caagaacttc tacggtggca atggcattgt tggggcccag gtacccctgg gagctggtgt
     1141 ggcttttgcc tgtaaatacc tgaagaatgg tcaggtctgc ttggctttgt acggcgatgg
     1201 tgcggctaac caagggcagg tattcgaagc atacaatatg tcagccttgt ggaaattacc
     1261 ctgtgttttc atctgtgaga ataacctcta tggaatggga acctccaacg agagatcagc
     1321 agccagtact gattaccaca agaaaggttt tattatcccc ggactgaggg tgaatgggat
     1381 ggatattctc tgtgttcggg aggcaaccaa gtttgcagct gatcactgca gatctggaaa
     1441 ggggcccatt gtgatggagc tgcagaccta ccgttatcat ggacacagta tgagcgaccc
     1501 agggatcagt tatcgttcac gagaagaagt tcataacgtg agaagtaaga gtgatcctat
     1561 aatgctgctc cgagagagaa ttatcagcaa caacctcagc aatattgaag aattgaaaga
     1621 aattgatgca gatgtgaaga aagaggtgga ggacgcagct cagtttgcta cgactgatcc
     1681 agaaccagct gtggaagata tagccaatta cctctaccac caagatccac cttttgaagt
     1741 ccgtggtgca cataagtggc tcaagtataa gtcccacagt tagatagatg ttacctatac
     1801 atttgttaaa ttttttttca gtgggacatt tatggtgtac tctaggaaac ttcaactttg
     1861 ttaaggagga ataaataaaa cgacattgca gacaaaagtc ttataaacct ttataaagat
     1921 agattcctga gttattaagg agattagaag atataaattt gtttaaagga gatgttccac
     1981 tttctgtttt aacattaaaa gcattgtgtt gcatactact atgaatatct tttagactat
     2041 ttcaaattta taaaattata atagaaaaaa cgggttaaat tccccaattt ggcatagtag
     2101 tttcatttgg ttttagtttt gaaataaata cttttattta aaagaagtag atttttgatt
     2161 ttttcttctt ctttgatgag tttgtcaaaa attaaaagtg aggaggatgg tttcaattct
     2221 gtccccgccc ccccctcacc aaatacctga ttatgttccc ctttccccct ccatgtccaa
     2281 tctctgaccc aggttcctcc ctccctctgt cccctgtggt tgatttttcc tcctaagtag
     2341 gattcaggc
//