From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: D.pseudoobscura Adh gene for alcohol dehydrogenase
Date: 28 Dec 92 21:26:38 GMT
Distribution: bionet
Checksum: 47221 8

LOCUS       DROADHGN18   3485 bp ds-DNA             INV       01-DEC-1992
DEFINITION  D.pseudoobscura Adh gene for alcohol dehydrogenase
ACCESSION   X62198
KEYWORDS    ADH gene; alcohol dehydrogenase.
SOURCE      Drosophila pseudoobscura DNA.
  ORGANISM  Drosophila pseudoobscura
            Eukaryota; Animalia; Metazoa; Arthropoda; Uniramia; Insecta;
            Pterygota; Neoptera; Holometabola; Diptera; Brachycera;
            Cyclorrhapha; Schizophora; Drosophiloidea; Drosophilidae.
REFERENCE   1  (bases 1 to 3485)
  AUTHORS   Schaeffer,S.W. and Miller,E.L.
  TITLE     Estimates of gene flow in Drosophila pseudoobscura determined from
            nucleotide sequence analysis of the alcohol dehydrogenase region
  JOURNAL   Genetics 132, 471-480 (1992)
  STANDARD  full automatic
COMMENT     *source: strain=GB119; *source: isolate=Gundlach Bundschu Winery,
            Sonoma Valley, *source: California; *source: chromosome=IV section
            88;
               From EMBL    entry DPADHGN18;  dated 16-OCT-1992.
FEATURES             Location/Qualifiers
     mRNA            join(40..119,915..1020,1084..1488,1548..1963)
                     /note="adult mRNA (Adh)"
     mRNA            join(882..1020,1084..1488,1548..1963)
                     /note="larval mRNA (Adh)"
     promoter        8..14
                     /note="adult promoter (Adh)"
     prim_transcript 40..1963
                     /note="adult primary transcript (Adh)"
     exon            40..119
                     /note="adult exon 1a"
     intron          120..914
                     /note="adult intron"
     promoter        846..853
                     /note="larval promoter (Adh)"
     prim_transcript 882..1963
                     /note="larval primary transcript (Adh)"
     exon            882..1020
                     /note="larval exon 1"
     exon            915..1020
                     /note="adult exon 1b"
     intron          1021..1083
                     /number=1
     exon            1084..1488
                     /number=2
     intron          1489..1547
                     /number=2
     exon            1548..1963
                     /number=3
     polyA_signal    1940..1945
     polyA_site      1963
     CDS             join(928..1020,1084..1488,1548..1814)
                     /EC_number="1.1.1.1"
                     /gene="Adh"
                     /product="alcoholdehydrogenase"
                     /codon_start=1
                     /translation="MSLTNKNVVFVAGLGGIGLDTSRELVKRNLKNLVILDRIDNPAA
                     IAELKAINPKVTITFYPYDVTVPVAETTKLLKTIFAQVKTIDVLINGAGILDDHQIER
                     TIAVNYTGLVNTTTAILDFWDKRKGGPGGIICNIGSVTGFNAIYQVPVYSGSKAAVVN
                     FTSSLAKLAPITGVTAYTVNPGITKTTLVHKFNSWLDVEPRVAEKLLEHPTQTSQQCA
                     ENFVKAIELNKNGAIWKLDLGTLEPITWTQHWDSGI"
BASE COUNT      971 a    795 c    796 g    923 t
ORIGIN      
        1 cgtagtgtat ttaaggcact gcacatgtcg aggaccacga ttattgtcta cagtgcagtc
       61 agtagttgtc aggtgcagca ggcggaccag catttcttta aatctactca atcgatcaag
      121 taagtaactg taatccaagt acagtcggcc catcccactc ggttccgcag tcactcaaga
      181 aatttcttga tagcgcagtt ccagttgtaa aacgtcgttt aaaaaaacat ctcgttggag
      241 cttgggttga aaaacgttcg agttgggcgt agaaaatttc aaatataaaa tatcaaaaaa
      301 gagaaaatat cttcccaaag cagctgtcca ttttttgtta aagtgataga tcaaaaattc
      361 gtatgacgta tttgccaagt gctctttatg caaataattt cctcaattct aattctacaa
      421 gaagcacttt tggctcttgt ccagaaaaaa caatttgttt acacttttcg gtttataaca
      481 gaagcggacc gacggctaag taacataaca gtcgcctgtt aagtaatgcc ctcactctct
      541 gttagatggc cgcgcgtgtc gactccgata gaccagcaga cacgcagtag ttttcctcaa
      601 gaacctgaac tctaaacata gacataattt acgatacctt gagactcaaa taaaaataca
      661 agtatgagtg gtattctatt cctgattaga ctcgcacaca cacacgtacg agtattaaca
      721 taaatttgcc ggcaaataac caaaacaaaa cgaacgcaaa cgatccgaac acgtaatgcg
      781 agagataaga aacagaaaag ctcgacgtga aagaagcttc tggcggaagc tcttgccgaa
      841 ctccctataa atacgagaca gaaaaccaga ataaatataa taacaaactg ttgaattgtc
      901 ccatcgattc ccagatcaaa agacagaatg tctctcacca acaagaacgt cgttttcgtg
      961 gccggtctgg gcggcattgg cctggacacc agccgggagt tggtcaagcg taatctgaag
     1021 gtaagagaga acgaattcca tggaatctat ggaatcctaa atttaaaaat ttattttttt
     1081 tagaacctgg tcatcctgga tcgcattgac aatccggctg ccattgccga gctgaaggca
     1141 atcaatccca aggtgaccat caccttctat ccctacgatg tgactgtgcc cgtcgctgag
     1201 accaccaagc tcctgaagac catctttgcc caggtgaaga caatcgatgt cctgatcaac
     1261 ggtgctggca tcctggacga tcatcagatc gagcgcacca ttgccgttaa ctacacgggc
     1321 ctggtcaaca ccaccacagc cattctggac ttctgggaca agcgcaaggg cggcccaggc
     1381 ggcatcattt gcaacattgg ctccgtcacc ggtttcaatg ccatctacca ggtgcccgtt
     1441 tactccggct ccaaggcggc ggtggttaac tttacctcct ccctggcggt aagtaatcca
     1501 actttttcct actccccaaa actaaatatt tctttgaaat cccatagaaa ctggctccca
     1561 ttactggtgt cactgcttac actgtcaatc ctggcatcac taagaccact ctggtccaca
     1621 aattcaactc gtggctggat gtggagcccc gtgtggcaga gaagctgctc gagcatccca
     1681 cccagacctc tcagcagtgc gccgagaact ttgtgaaggc cattgagctg aacaagaacg
     1741 gtgccatctg gaagttggat ttgggcacct tggagcccat cacatggacc cagcactggg
     1801 actcgggcat ctaaaccatc ccagagactc tatgggacat ggcgttttag cttttagttt
     1861 ctttttttcc actcaattgt tacgtatata catacacata tggcaataag gctgatttga
     1921 accccgtttt gaatatgata atacaaaatt atatttgaga aatttcaaca aaatcgataa
     1981 gagctatagc tctcttttaa ttaaaaaaaa ctagataaaa agagcaatgg tcagtggtgg
     2041 tgggcagtgg tcgttgtggc ctctatcgat ttccacacaa aaactttact gtttagtaat
     2101 agaaaagaac ttgagaaagg cagccaaaag aatgtacgat ctgacgggta agcatgtctg
     2161 ctatgtggct gactgcggtg gcattgcact ggagaccagc aaggttctca tgaccaagaa
     2221 tatagcggtg agtggtagag aggtggtgta gagagtggag agtggttctg gttcttaaac
     2281 aagtctctag tctctagtct ctagtctcta gtctcttggc tgctgctggt agctgtcgaa
     2341 actaattaag ttaatgactt tttcatgttt tgtttttgtc cgctgacttt tgctgttcaa
     2401 agctacaatt aagtttatgt tttgagtttg atctactttt gcttaaggga agtactttcg
     2461 attacggatt gctggcatat aaaaatagat accaaggaat gtgcctcata tcatattgat
     2521 tctatcttga ttctttgccc agaaactggc catccttcag agcgtggaaa accccccggc
     2581 catcgctcag ttacaatcga ttaagcacag cacacagatc ttcttctgga ccttcgatgt
     2641 gaccatggcc cgcgaggaga tgaagaagta cttcgatgag gtcatggtcc agatggacta
     2701 catcgatgtg ctgatcaatg gggccaccct gtgcgatgag cggaacattg atgccaccat
     2761 caatacgaat ctgaccggca tgatgaacac cgtggccact gtgctgccct atatggaccg
     2821 aaagatgggc ggttcgggtg gactgattgt gaatgtcacc tccgtcatag gactggatcc
     2881 atcgccagtc ttttgtgcgt acagtgcctc aaagtttggt gtgattgggt tcacccgaag
     2941 tctagcggtg agtgggcgag atcttaactt gaaaatgctc tctaatgtct tccattcttt
     3001 cgcaggatcc cctttattac acccaaaatg gtgtggctgt catggccgtc tgctgtggtc
     3061 ccaccaaagt gtttgtcgat cgggaactga ctgccttcct ggaatacggc cagtcctttg
     3121 ccgatcgcct gcgtcgtgcc ccctgccaat cgacggccgt ctgcggccag aatatcgtaa
     3181 atgccatcga gagatcggaa aacggacaga tctggattgc cgacaagggc ggcctggagt
     3241 cggtggccct ccactggtat tggcatatgg ccgatcagtt tgtgaactat atgcagagca
     3301 ctgacgatga ggatcaggag ttttttttgg gccagcgatg aggcatgcct tgatccacgg
     3361 aataaaacat caaatgtgtc acaaaaagta ggcaacttgt ttgtgggtct ggaaatagat
     3421 ttgaaatatt ttgaggtcat atgaataatg acataaatga gggtgcctga aagtatgcca
     3481 agttt
//