From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Bacillus alcalophilus gene for NADH dehydrogenase alpha-2 subunit,
Date: 21 Jul 92 10:03:34 GMT
Distribution: bionet
Checksum: 13575     6

LOCUS       BACNDH       2354 bp ds-DNA             BCT       21-JUL-1992
DEFINITION  Bacillus alcalophilus gene for NADH dehydrogenase alpha-2 subunit,
            complete cds.
ACCESSION   D10701 D01111
KEYWORDS    NADH dehydrogenase; alkalophile; membrane protein.
SOURCE      Bacillus alcalophilus (strain YN) DNA.
  ORGANISM  Bacillus alcalophilus
            Prokaryotae; Firmicutes; Endospore-forming rods and cocci;
            Bacillaceae.
REFERENCE   1  (bases 1 to 2354)
  AUTHORS   Xu,X., Koyama,N., Cui,M., Yamagishi,A., Nosoh,Y. and Oshima,T.
  TITLE     Nucleotide sequence of the gene encoding NADH dehydrogenase from an
            alkalophile, Bacillus sp. strain YN-1
  JOURNAL   J. Biochem. 109, 678-683 (1991)
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     CDS             <1..507
                     /product="ORF"
                     /codon_start=1
                     /translation="EFIEVSEESFKGQWSVLCFYPADFTFVCPTELEDLQNEYAALKE
                     LGVEVFSASTDTHFTHKGWHDSSETIGKITYAMIGDPSQTLSRNFDVLNEVSGLADRG
                     TFIIDPDGVVQAAEINAEGIGRDASTLVNKIKAAQYVRNNPGEVCPAKWQEGDETLKP
                     SLDLVGKI"
     RBS             505..513
     CDS             520..2079
                     /product="NADH dehydrogenase, alpha-2 subunit"
                     /gene="ndh"
                     /evidence=EXPERIMENTAL
                     /codon_start=1
                     /translation="MVLEPQIKSQLNQYLQLMEGDVLLKVSAGNDKVSEDMLSLVDEL
                     ASMSSRITVEKTNLERTPSFSVNRPGEDTGIVFAGIPLGHEFTSLVLALLQVSGRAPK
                     AEQNVIDQIKNIEGEYHFESYISLSCQNCPDVVQALNLMSVLNPGISHTMIDGAAFKD
                     EVESKDIMAVPTVYLNGESFTSGRMTVEEILAQLGSGPDASELADKDPFDVLVVGGGP
                     AGASSAIYAARKGIRTGIVADRFGGQIMDTLSIENFISQKYTEGPKLAASLEEHVKEY
                     DIDVMKLQRAKRLEKKDLIEIELENGAVLKSKSVILSTGARWRNVGVPGEQEFKNKGV
                     AYCPHCDGPLFEGKDVAVIGGGNSGVEAAIDLAGIVNHVTVLEFMPELKADEVLQERL
                     NSLPNVTVIKNAQTKEITGDDKVNGISYMDRDTEEVHHIELAGVFVQIGLVPNTDWLD
                     GTLERNRFGEIVVDSHGATNVPGVFAAGDCTNSAYKQIIISMGSGATAALGAFDYLIR
                     NTTPAESAAAK"
     repeat_region   2074..2102
                     /rpt_type=inverted
                     /rpt_unit=2074..2082
                     /rpt_unit=2094..2102
     repeat_region   2103..2144
                     /rpt_type=inverted
                     /rpt_unit=2103..2120
                     /rpt_unit=2126..2144
                     /note="it could form a stable stem-loop structure."
BASE COUNT      692 a    478 c    561 g    623 t
ORIGIN
        1 gaattcatcg aggtatcaga agaaagcttt aaaggccagt ggagcgttct ttgtttctac
       61 cctgcagact tcacttttgt ctgcccaaca gagcttgaag accttcaaaa tgagtatgct
      121 gctttgaaag aacttggagt ggaagtattc tcagcttcta cagatactca ctttactcat
      181 aaaggctggc atgacagctc tgaaacaatc ggaaaaatca cttacgctat gatcggtgat
      241 ccttcacaga cactttcccg taactttgat gtactgaacg aagtgtccgg tcttgctgat
      301 cgcggtacat tcatcatcga tccagacggt gtagtacaag ctgcggaaat taacgctgaa
      361 ggcatcggcc gtgatgcaag tactcttgtt aataaaatta aagcagcaca atatgtaaga
      421 aataaccctg gtgaagtttg cccggctaaa tggcaagagg gagacgaaac acttaagcca
      481 agcctggatc tggtagggaa aatctaagga gtgctataaa tggtacttga accgcaaatt
      541 aaatcacaat taaaccaata cttgcagctt atggaagggg acgtccttct taaagtaagc
      601 gcaggaaatg ataaagtttc tgaggatatg ctctctcttg tcgacgaact tgcttctatg
      661 tcttccagga ttactgttga aaaaactaat ctggaaagaa ctccaagctt tagtgtaaac
      721 cggccagggg aagatactgg tattgtattt gctggtattc ctttagggca tgagttcact
      781 tcgcttgttt tagcattgct gcaggtgagc ggaagagctc ctaaagcaga acagaatgtt
      841 atcgaccaga tcaaaaatat cgaaggcgaa tatcattttg aatcttatat cagcctgagc
      901 tgccaaaact gccctgacgt agtacaggca cttaacttaa tgagtgttct taaccctggc
      961 atcagccata caatgatcga tggtgctgcc tttaaagatg aggtagaaag caaagacatt
     1021 atggccgtgc caactgttta ccttaatggt gaatcattca cgagcggcag aatgacagtg
     1081 gaagagattc ttgcacagct gggaagcggc cctgatgcat cagaattagc tgacaaagat
     1141 ccattcgatg tactcgttgt cggcggcggt cctgcgggtg caagttctgc tatctatgct
     1201 gcccgtaaag ggattcgcac aggtatcgta gctgaccgtt tcggcggcca gattatggac
     1261 acgctgagca ttgaaaactt tatcagccag aagtacacag aaggaccaaa acttgcagca
     1321 agcctggaag agcacgtaaa agaatacgat atcgatgtaa tgaaacttca gcgtgctaaa
     1381 cgcctggaga aaaaagattt aattgagatc gagcttgaaa acggtgctgt tctgaaaagt
     1441 aagagcgtga ttctttctac tggagcacgc tggcgcaatg taggcgtacc tggagaacag
     1501 gagttcaaaa acaaaggggt agcttactgc cctcactgtg atggtcctct gtttgaagga
     1561 aaagacgttg cagttatcgg tggaggtaac tctggtgtag aagctgcaat cgaccttgca
     1621 ggtattgtaa accatgtgac ggttcttgag ttcatgcctg agctgaaagc tgacgaagtg
     1681 ctgcaggaac gcttaaacag cctgccaaac gtcactgtaa ttaaaaatgc tcagacgaaa
     1741 gaaattaccg gcgatgacaa agtaaacggt atttcttaca tggaccgcga tacggaagaa
     1801 gtacaccata ttgaattagc cggggtattc gttcagatcg ggcttgtacc taacactgac
     1861 tggctagacg ggactcttga acgtaatcgt ttcggtgaaa tcgtcgtaga ttctcatggt
     1921 gctacaaatg tacctggggt gtttgcggca ggagactgta ctaacagtgc ctacaagcaa
     1981 atcattattt ctatgggatc cggtgctaca gctgcattag gtgcattcga ttacctgatt
     2041 agaaatacaa cgccagcaga aagtgctgcg gctaaataat ttaaagataa cgcaatttat
     2101 ttaagaagcc acttcctatc aaaacgatag tgaggcggct tcttttttct cgtctcataa
     2161 ggttttgaga tgtacaccat ggagcataag ccgtggaagt atgctttata agttcatatt
     2221 attactgcat ctcttgccat tctcattggg cccattcagt tccttctgaa taaaacaagg
     2281 tcccgcagac tttccataca tcgatctctg ggtaagttat atgttatagc tatagccata
     2341 agtgggtgct gtgt
//