From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Bacillus subtilis csbA and uvr/dinA genes, complete cds.
Date: 15 Sep 92 10:02:17 GMT
Distribution: bionet
Checksum: 10858     5

LOCUS       BACCSBA      1353 bp ds-DNA             BCT       15-SEP-1992
DEFINITION  Bacillus subtilis csbA and uvr/dinA genes, complete cds.
ACCESSION   M80473
KEYWORDS    cytoplasmic protein; excision repair; membrane protein;
            mitomycin C sensitivity; regulon.
SOURCE      Bacillus subtilis (strain 168, sub_species Marburg) DNA.
  ORGANISM  Bacillus subtilis
            Prokaryotae; Firmicutes; Endospore-forming rods and cocci;
            Bacillaceae.
REFERENCE   1  (bases 1 to 1353)
  AUTHORS   Boylan,S.A., Thomas,M.D. and Price,C.W.
  TITLE     Genetic method to identify regulons controlled by nonessential
            elements: Isolation of a gene dependent on alternate transcription
            factor sigma-B of Bacillus subtilis
  JOURNAL   J. Bacteriol. 173, 7856-7866 (1991)
  STANDARD  simple staff_entry
FEATURES             Location/Qualifiers
     promoter        260..297
                     /note="sigma-B dependent"
                     /gene="csbA"
                     /evidence=EXPERIMENTAL
     mRNA            260..667
                     /note="sigma-B dependent promoter transcript"
                     /gene="csbA"
                     /note="putative"
     promoter        316..351
                     /note="sigma-B independent"
                     /gene="csbA"
                     /evidence=EXPERIMENTAL
     mRNA            316..667
                     /note="sigma-B independent promoter transcript"
                     /gene="csbA"
                     /note="putative"
     RBS             367..372
                     /gene="csbA"
     CDS             380..610
                     /note="putative membrane protein"
                     /gene="csbA"
                     /note="putative"
                     /codon_start=1
                     /translation="MITKAVFALFFPFMLVVLFTRVTFNHYVAIALTAALLFASYLKG
                     YTETYFIVGLDVVSLVAGGLYMAKKAAEKKEE"
     terminator      640..667
                     /gene="csbA"
                     /evidence=EXPERIMENTAL
     RBS             782..786
                     /gene="uvr/dinA"
     CDS             796..1353
                     /note="putative cytoplasmic protein"
                     /gene="uvr/dinA"
                     /note="putative"
                     /codon_start=1
                     /translation="MKDRFELVSKYQPQGDQPKAIEKLVKGIQEGKKHQTLLGATGTG
                     KTFTVSNLIKEVNKPTLVIAHNKTLAGQLYSEFKEFFPNNAVEYFVSYYDYYQPEAYV
                     PQTDTFIEKDASINDEIDKLRHSATSALFERRDVIIIASVSCIYGLGSPEEYREMVVS
                     LRTEMEIERNELLRKLVAIQYARNDI"
                     /partial
BASE COUNT      414 a    227 c    302 g    410 t
ORIGIN
        1 tttaaaaaat gatagtaaac actatcattt aattgtaggt tttcaggaaa atgctgtcaa
       61 acgcagaaag tcctaaataa aatgaatagt taagttggtt atattatcct gaaaaatgcg
      121 gccgtactgg tactgtatgc ctattttctt ttattctcgg aatctggcgg cggacttgat
      181 gatgtaataa aaaaagtgcc gctgattaga acagcggcac catggaataa gttttgaccg
      241 gcaccaaaat gcaaatatgg tgattgattt tggctgaaaa ggggtatggt gtaagtagag
      301 ggagcgaatg cacgattgtc cgattcttca ttttttacta taatcagatc agatgaagaa
      361 tccctgagga gggtttttgt tgattacaaa agccgttttt gcattgtttt tcccttttat
      421 gcttgttgtt ctatttacta gagtcacctt taatcattat gtggcgatcg ctttaacagc
      481 tgcattgctg tttgcctctt atttaaaagg ctatacagaa acgtatttta ttgtaggatt
      541 ggatgttgtg tctcttgtgg ctggcggact gtatatggcc aaaaaagctg cagagaaaaa
      601 agaagaataa atcggacata atgaatataa agactgaata cctgctttta cgttttaaaa
      661 gcaggttttt tatacacaaa aacagctgga aataaaaaac caccgaactt tagttcgtat
      721 ttttagtgat tttgctttcc attgtgttac tatatctata ggaagatttc gttaaagaaa
      781 cggaggctta tttttgtgaa agatcgcttt gagttagtct cgaaatatca gccccaggga
      841 gatcagccga aagccattga aaagcttgtg aaaggaattc aggagggcaa gaagcatcag
      901 actctgctgg gtgcaacagg aactgggaaa acatttacgg tgtccaattt gattaaagaa
      961 gtcaataagc cgactctcgt gattgcccat aacaaaaccc ttgctggaca gctttacagt
     1021 gagttcaagg aattttttcc gaacaatgct gtcgagtatt ttgtcagcta ctatgattat
     1081 tatcagccgg aggcgtatgt gcctcaaacg gatacattta tagagaaaga cgctagtatt
     1141 aatgatgaaa ttgataaact gagacactcc gccacatcag ctttgtttga gcgcagagat
     1201 gtcattatta ttgcgagtgt gtcttgtata tacggcctcg gttcgcctga ggaataccgg
     1261 gaaatggtcg tgtcactgcg gactgaaatg gaaattgagc gcaacgagct gctcagaaaa
     1321 cttgtagcaa tccaatatgc ccgcaacgat atc
//