From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: histidine biosynthesis operon: hisD=histidinol dehydrogenase,
Date: 27 Jan 93 05:55:26 GMT
Distribution: bionet
Checksum: 17638 6

LOCUS       S117731      2298 bp    DNA             BCT       12-JAN-1993
DEFINITION  histidine biosynthesis operon: hisD=histidinol dehydrogenase,
            hisC=histidinol-phosphate aminotransferase [Mycobacterium
            smegmatis, ATCC 607, Genomic, 2298 nt]
ACCESSION   S48356 X65542
KEYWORDS    .
SOURCE      Mycobacterium smegmatis ATCC 607
  ORGANISM  Mycobacterium smegmatis
            Unclassified.
REFERENCE   1  (bases 1 to 2298)
  AUTHORS   Hinshelwood,S. and Stoker,N.G.
  TITLE     Cloning of mycobacterial histidine synthesis genes by
            complementation of a Mycobacterium smegmatis auxotroph.
  JOURNAL   Mol. Microbiol. 6, 2887-2895 (1992)
  STANDARD  full automatic
COMMENT     This entry [NCBI gibbsq 117731] was created by the journal scanning
            component of NCBI/GenBank at the National Library of Medicine.
            This sequence comes from Fig. 3.
            Region: histidine biosynthesis operon.  
FEATURES             Location/Qualifiers
     CDS             305..1642
                     /note="HisD;  For the protein sequence (NCBI gibbsq
                     117732):  Method: conceptual translation supplied by
                     author.  This sequence comes from Fig. 3."
                     /gene="hisD"
                     /product="histidinol dehydrogenase"
                     /codon_start=1
                     /translation="MNMMAKFQMSRIDLRNRVLSAAQLRSALPRGGVDVDAVVPKVRP
                     IVDAVAQRGAQAALEYGESFDGIRPETVRVPRELLSQALENLDADVRAALQVSIDRAR
                     AVHADQRRTDTTTTLAPGATVTERWVPVERVGLYVPGGNAVYPSSVVMNVVPAQTAGV
                     DSMVIASPPQGQFGGRPHPTILAAAALLGVDEVWAVGGAQAVALLAYGGTDTDGAELA
                     PVDMITGPGNIYVTAAKRICRSQVGIDAEAGPTEIAILADHTADPVHVAADLISQAEH
                     DEMAASVLVTDSETLAEATDRELANQLATTKHVERVTAALSGKQSAIVLVDDIDAGVR
                     TVNAYAAEHLEIQTVDAPGVAGRIRSAGAIFVGAWSPVSLGDYCAGSNHVLPTAGCAR
                     HSSGLSVQTFLRGIHVVEYDEAALKDVSGHVITLSKAEDLPAHGEAVRRRFER"
     CDS             1642..2298
                     /partial
                     /note="HisC;  For the protein sequence (NCBI gibbsq
                     117734):  Method: conceptual translation supplied by
                     author.  This sequence comes from Fig. 3."
                     /gene="hisC"
                     /product="histidinol-phosphate aminotransferase"
                     /codon_start=1
                     /translation="MSADKVTLADLPLRDNLRGKSPYGAPQLQVPVRLNTNENPHPPS
                     KALVDDVAASVREAAAELHRYPDRDAVALRTDLAAYLTAATGVRLGVENLWAANGSNE
                     ILQQLLQAFGGPGRTAIGFVPSYSMHPIISDGTQTEWLQASRAEDFGLDIDVAVSAVT
                     ERKPDVVFVTSPNNPSGQSVPLDDLRRVLDAMQGGILIVDEAYGEFSSQPSAVALLDD
                     Y"
BASE COUNT      361 a    805 c    772 g    360 t
ORIGIN      
        1 gatcaccgcg aacacattgt ccttgcggta actgttgacc acggtctcgt acgcccggcc
       61 cgacttgcgg ccccggtgct tgatgacggt gaaaccgggt agtcgtttgg cgacgggcgt
      121 catcaccgga ttgacgtatt tgagttgcag tcgctccagc cagggcggga acagcatcgg
      181 tacgccaggt gccttgttgg ggtggtcttt tgccgacaac tcgggctccg ttcgcgggtg
      241 ccgcgaccac ccggaggttg acggtggccg acggattttt attcaagtgg actgatcagg
      301 gacaatgaac atgatggcca agtttcagat gtcccgcatc gacctgcgca accgtgtgct
      361 cagcgcggcg caactgcgct ccgccctgcc gcgcggtgga gtcgacgtcg acgccgtggt
      421 gcccaaggtc cgcccgatcg tcgacgcggt cgcacagcgg ggcgcgcagg ccgccctgga
      481 gtacggcgaa tccttcgacg gcatccgccc ggagacggtg cgggtgccgc gcgagttgct
      541 cagccaggcg ctggagaacc tcgacgccga cgtgcgcgcc gcgctgcagg tctccatcga
      601 ccgtgcgcgt gccgtgcatg ctgatcagcg tcgcaccgac accaccacca cactggcgcc
      661 cggcgccacc gtgaccgagc gctgggttcc ggtcgagcgg gtcggcctct acgttccggg
      721 cggcaacgcc gtctatccgt cgagcgtcgt gatgaacgtc gtgcccgccc agaccgcggg
      781 cgtggactcg atggtcatcg ccagtccgcc gcagggccag ttcggcggca ggccgcaccc
      841 gaccatcctc gccgcggccg cgttgctcgg tgtcgacgag gtgtgggcgg tcggcggcgc
      901 acaggccgtg gcgcttttgg cctacggcgg caccgacacc gacggcgccg aactcgcgcc
      961 ggtcgacatg atcaccggtc cgggcaacat ctacgtgacg gccgccaagc gcatctgccg
     1021 ctcccaggtc ggcatcgacg ccgaggccgg ccccaccgag atcgcgatcc tggccgacca
     1081 cacggccgat ccggtgcacg tggccgccga tctgatcagc caggccgagc acgacgagat
     1141 ggccgccagc gtgctggtca ccgacagtga gacgctggcc gaggccaccg accgcgaact
     1201 ggccaaccag ctcgccacca ccaagcacgt cgagcgggtc accgcggcac tctcgggcaa
     1261 acagtccgcg atcgttctgg tcgacgacat cgacgcgggc gtgcgcaccg tcaacgccta
     1321 cgccgccgag catctcgaga tccagaccgt cgacgccccc ggcgtggccg gccggatccg
     1381 ttcggccgga gccattttcg tgggcgcctg gtcaccggtg agcctcggcg actactgcgc
     1441 cgggtccaac cacgtgctgc ccaccgcggg atgcgcgcgg cactccagtg ggctgtcggt
     1501 gcagacgttc ctgcgtggca tccacgtcgt cgagtacgac gaggcggcgc tcaaagacgt
     1561 ttcggggcac gtgatcacgc tgtccaaggc cgaggatctg cccgcgcacg gcgaggcggt
     1621 acgacggagg ttcgagcggt aatgtctgct gacaaggtga cgttggcgga tctgccgctg
     1681 cgcgacaacc tgcggggcaa gtcgccgtac ggtgcgccgc aactgcaggt tccggtgcgc
     1741 ctgaacacca acgagaaccc gcatccgccc tccaaggcgc tcgtcgacga cgtcgcggcc
     1801 tcggtccgcg aggccgccgc cgaactgcac cgctaccccg atcgggacgc ggtcgcgctg
     1861 cgcaccgatc tcgcggcgta cctcacggcc gccaccggcg tccggttggg tgtcgaaaac
     1921 ctttgggcgg caaacggttc caatgagatc ctgcagcagc tgctgcaggc gttcggcggc
     1981 ccgggccgca ccgccatcgg cttcgtgccg tcgtactcga tgcacccgat catctccgac
     2041 ggcacgcaga ccgagtggct gcaggcctcg cgcgccgagg atttcggcct cgacatcgac
     2101 gttgcggtgt ccgcggtcac cgagcgcaaa cccgacgtgg tgttcgtgac gagccccaac
     2161 aacccgtcgg gccagagcgt tccgctcgac gatctgcgcc gcgtgctcga cgcgatgcag
     2221 ggcgggatac tcatcgtcga cgaggcctac ggcgagttct cgtcacagcc cagcgcggtg
     2281 gcgctgctcg acgactat
//