From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: T.aestivum PRK gene for ribulose-5-phosphate kinase
Date: 10 Sep 92 10:36:23 GMT
Distribution: bionet
Checksum: 20590     6

LOCUS       WHTPRK       2501 bp ds-DNA             PLN       10-SEP-1992
DEFINITION  T.aestivum PRK gene for ribulose-5-phosphate kinase
ACCESSION   X57952
KEYWORDS    chloroplast protein; phosphoribulokinase; prk gene;
            ribulose-5-phosphate kinase.
SOURCE      Triticum aestivum DNA.
  ORGANISM  Triticum aestivum
            Eukaryota; Plantae; Embryobionta; Magnoliophyta; Liliopsida;
            Commelinidae; Cyperales; Poaceae.
REFERENCE   1  (bases 1 to 2501)
  AUTHORS   Lloyd,J.C., Horsnell,P.H., Dyer,T.A. and Raines,C.A.
  TITLE     Structure and sequence of a wheat phosphoribulokinase gene.
  JOURNAL   Plant Mol. Biol. 17, 167-168 (1991)
  STANDARD  full automatic
COMMENT     *source: strain=Chinese spring; *source: stage=8 day; *source:
            tissue=etiolated shoots; *source: clone_library=genomic in lambda
            charon 35; *source: chromosome=6AL, 6BL, 6DL;
            See also X07780
            From EMBL    entry TAPRK;  dated 02-SEP-1991.
FEATURES             Location/Qualifiers
     mRNA            join(157..771,1029..1113,1201..1285,1379..1623,1860..2364)
                     /gene="PRK"
     CDS             join(224..771,1029..1113,1201..1285,1379..1623,1860..2111)
                     /EC_number="2.7.1.19"
                     /product="ribulose-5-phosphate kinase"
                     /gene="PRK"
                     /codon_start=1
                     /translation="MAFCSPHSTTSLRSPCTTIPNSGFRQNQVILFTTRSSRRSNTRH
                     GARTFQVSCAVEQPIVIGLAADSGCGKSTFMRRLTSVFGGAAEPPKGGNPDSNTLISD
                     TTTVICLDDYHSLDRTGRKEKGVTALDPKANDFDLMYEQVKAIKEGKAIEKPIYNHVT
                     GLLDPAELIQPPKIFVIEGLHPMYDERVRELLDFSIYLDISNEVKFAWKIQRDMAERG
                     HSLESIKASIEARKPDFDAFIDPQKQYADAVIEVLPTQLIPDDNEGKVLRVKLIMKEG
                     IKFFNPVYLFDEGSTINWIPCGRKLTCSYPGIKFSYGPDTYFGQEVSVLEMDGQFDRL
                     DELIYVESHLSNLSTKFYGEVTQQMLKHADFPGSNNGTGLFQTIVGLKIRDLYEQIIA
                     ERAGVPAEAAKV"
     mat_peptide     join(383..771,1029..1113,1201..1285,1379..1623,1860..2107)
                     /EC_number="2.7.1.19"
                     /product="ribulose-5-phosphate kinase"
                     /gene="PRK"
                     /codon_start=1
     exon            157..771
                     /number=1
     transit_peptide 224..382
                     /gene="PRK"
     intron          772..1028
                     /number=1
     exon            1029..1113
                     /number=2
     intron          1114..1200
                     /number=2
     exon            1201..1285
                     /number=3
     intron          1286..1378
                     /number=3
     exon            1379..1623
                     /number=4
     intron          1624..1859
                     /number=4
     exon            1860..2364
                     /number=5
BASE COUNT      736 a    584 c    528 g    653 t
ORIGIN
        1 gatcactata agctggccct gatatcacca gaattccaga aaagaaaaag agaaaaaagg
       61 ggaaataggg acaccacatc atcaccatct catttccctc cttatgtttt cttagctctt
      121 atctccacag ttctttcatc aagctctctt ctccccaagt ctcagttaca acttacaagg
      181 cttgaagggg agaagagcag ctccaagagg tcgggcagca aacatggcat tctgcagccc
      241 acacagcacc acatccctgc gctccccatg caccaccatc ccaaactctg gcttcaggca
      301 gaaccaagtc atcctcttca cgaccagaag cagcaggagg agtaacacga ggcatggggc
      361 aaggaccttc caggtctcat gcgcagttga gcagccaata gtgattggcc tggcagcaga
      421 ctcgggatgt gggaaatcca ccttcatgcg ccggctcacc agcgtgtttg gaggtgctgc
      481 agagccaccc aagggcggca acccagactc caacacgctc atcagcgaca cgacgacggt
      541 catatgcctt gatgactacc attccttgga cagaaccggg aggaaggaga aaggtgtgac
      601 cgccctggac cccaaggcaa acgactttga tctcatgtat gagcaggtga aggcaatcaa
      661 ggaaggcaag gctattgaga agccaatcta caaccacgtc actggcctcc tcgacccggc
      721 ggagctcatc cagcccccca agatcttcgt catcgagggt ttgcacccaa tgtgagttca
      781 cattctactc ccatgcttct cctgtctgtt tcagtatttg gtgtctgttt tttctttctt
      841 tcttcctcat attccagtag ctgttgggta agtggtcatc ataaaaacct tttgaatcac
      901 agcagcaact tctgttaagt ttccacaaaa tattcgattc cctgatgctt aacagttagc
      961 agtatttccc tgactaaaat aaacatgatc attttgcaag ctgtaacaaa cagaaatttt
     1021 taaaccaggt acgatgaacg tgtgagagag ctccttgatt tcagcatcta cttagacatc
     1081 agcaatgagg ttaagttcgc atggaaaatt caggtgagct gaacagtaag gtttagatgt
     1141 ttcaatggat tttgttgctg ttatctaact ttaaaaaaac aatcactggc acttttgcag
     1201 agagacatgg cagagcgtgg gcacagtctt gaaagcatca aggctagcat cgaagccagg
     1261 aaacctgatt ttgatgcctt tattggtact gcctttttac caactaatca cagcaagtgt
     1321 tttccataaa ccaaaaataa taaaagatgg tatgtaacct gaccacttgg tatgacagat
     1381 ccgcagaagc aatacgctga tgctgtgatt gaagttctgc caacccagct gattcctgat
     1441 gacaacgaag gaaaggtgct gcgagttaaa ttgatcatga aagaaggcat taagttcttc
     1501 aacccggttt acctcttcga tgaaggatca accatcaact ggatcccttg tggaagaaag
     1561 cttacatgct cttatcctgg catcaaattt tcctatggcc cagacactta ctttggccaa
     1621 gaggtactca tcttggctta tttacccttt tgtgtcaaac gagtaatgct aactgctgtt
     1681 acttggaaat aaatcaaaag aaacctagca agtagtccaa agtaggacaa agtaagagag
     1741 ctccaaaacc attcttagac tagagatcga ctattcactg agtagaacaa ttctagctgt
     1801 caaacaggtc ctgcctcaga caatgctgta caatcttagt gtttgcttgt ttatttcagg
     1861 tatcggtgct ggagatggat gggcaatttg acagactaga tgaactcatc tatgttgaga
     1921 gccacctaag caacctctca accaagttct acggggaggt gacacagcaa atgctaaagc
     1981 atgcagactt cccaggcagc aacaatggaa caggtctctt ccagaccatt gtaggactga
     2041 agattagaga tctctatgaa cagatcatcg ctgagagggc cggcgttcct gctgaagcag
     2101 caaaagtttg atgagcccca aattcaagtt cacgaatcat ttcatcacca actcaatgag
     2161 aatgagcatc ctcgcatttc ttgtacctag tttgaatata ttacagctag aagacggtag
     2221 aaagttcact gcagaactca tgaaaacctg cattttgaac ttctcgaaaa tcctctccat
     2281 gatgagggtc tcttatgtct cttcttaaca aatttcgatg tacatcttct cccatgtttc
     2341 aatgcagaaa atacatgtgc ttgctagaat caaaagcggc tgcttttatc tagtgtgtcc
     2401 tttttttggc tgttactgct gatcacgact aacattcata aaaggccatt taagaaattt
     2461 gaaaaaatgg tggtggattt gtagacagaa ttgtgaaaaa t
//