From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: B.licheniformis cwlM gene for N-acetylmuramoyl-L-alanine amidase
Date: 4 Sep 92 10:02:29 GMT
Distribution: bionet
Checksum: 27761     4

LOCUS       BACCWLM      1320 bp ds-DNA             BCT       04-SEP-1992
DEFINITION  B.licheniformis cwlM gene for N-acetylmuramoyl-L-alanine amidase
ACCESSION   X62116
KEYWORDS    N-acetylmuramoyl-L-alanine amidase; cwlM gene.
SOURCE      Bacillus licheniformis DNA.
  ORGANISM  Bacillus licheniformis
            Prokaryotae; Firmicutes; Endospore-forming rods and cocci;
            Bacillaceae.
REFERENCE   1  (bases 1 to 1320)
  AUTHORS   Kuroda,A., Sugimoto,Y., Funahashi,T. and Sekiguchi,J.
  TITLE     Genetic structure, isolation and characterization of a Bacillus
            licheniformis cell wall hydrolase
  JOURNAL   Mol. Gen. Genet. 234, 129-137 (1992)
  STANDARD  full automatic
COMMENT     *source: strain=FDO120;
            From EMBL    entry BLCWLM;  dated 10-AUG-1992.
FEATURES             Location/Qualifiers
     -35_signal      120..125
                     /note="promoter like I"
     -10_signal      142..147
                     /note="promoter like I"
     -35_signal      157..162
                     /note="promoter like II"
     -10_signal      181..186
                     /note="promoter like II"
     RBS             267..273
     CDS             280..1041
                     /EC_number="3.5.1.28"
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /gene="cwlM"
                     /evidence=EXPERIMENTAL
                     /codon_start=1
                     /translation="MVKIFIDPGHGGSDTGASANGLQEKQLTLQTALALRNMLLNEYQ
                     NVSVLLSRTSDQTVSLTQRTNAANSWGADYFLSIHMNAGGGTGFEDYIYPGVGAPTTT
                     YRDIMHEEILKVVDFRDRGKKTANFHVLRETAMPALLTENGFVDNTNDAEKLKSSAFI
                     QSIARGHANGLARAFNLSKNAAALYKVQIAAFRTKANADSLAAQAEAKGFDALVIYRD
                     SLYKVQIGAFSSKENAEALVQQAKNAEFDTFIYQE"
     terminator      1056..1094
                     /note="rho-independent"
BASE COUNT      381 a    316 c    284 g    339 t
ORIGIN
        1 ctgcagaaac gttcctgtcg tcctttcgag gaaggcatga cattccggtt ccgatataaa
       61 actccctatt acagcttaaa ggttaaaact taattatcat tacgtacaat gcagcgtaat
      121 tgaattatat gataaaaaca ttaaaaatgc aacctcttgc ctgaagagaa tatgacgctc
      181 tataataagt tgaatattta caaaattctg caatactgtg gcgttcgtac aacccttcat
      241 gtccatctgt tcatagactg tctattgagg tgatgtcttg tggtaaaaat ttttatcgat
      301 cccggccatg gcgggtctga tacaggtgct tcagctaatg gtttgcaaga aaaacagtta
      361 acattgcaaa ccgccttggc tttaagaaac atgctgctga atgaatatca aaatgtttca
      421 gtcctgttga gcaggacaag cgaccaaacc gtcagtctga cgcaacgcac gaacgccgcg
      481 aacagctggg gagcggacta ctttctatcg attcacatga atgccggggg cgggacaggt
      541 ttcgaggatt acatttatcc cggcgtcggc gcacctacca ccacctatcg ggatatcatg
      601 catgaagaga ttttaaaagt tgtcgatttc agggatcgcg gcaagaaaac ggccaatttt
      661 catgttctta gagaaacagc catgcccgca ctccttacgg aaaatggctt tgtagacaat
      721 acaaacgacg ccgagaaact gaagtcttcc gcgtttattc aatccattgc cagaggccac
      781 gccaacggac tcgcaagggc atttaacctt tccaaaaacg cagcagctct ctataaagtg
      841 cagatcgccg ctttcaggac caaagccaat gccgattcgc ttgccgctca agcggaagcg
      901 aaagggtttg atgcccttgt gatttacagg gacagccttt acaaagtgca aattggcgct
      961 ttttcttcca aagaaaatgc cgaagcgctc gttcagcaag ccaaaaacgc cgaatttgac
     1021 actttcattt accaggaata gggacaatca tcaagaaaag cccgcgatct gtctcgtata
     1081 gatcgcaggc tttttttatg cttttgcagc tttactttga atcgccggct tttgcgtttg
     1141 attcgatgca tggctgttca acaccattcc gataatcaca agtgccatgc cgctccatga
     1201 gatggcacca ggaagcggac ttgaaagaag caaaatttca ccagcggcag caaacagcac
     1261 ttcaaacgat tgtgtggcct cgacagcccc taattttcgc atatcccctt ttacaagatc
//