From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: D.pseudoobscura Adh gene for alcohol dehydrogenase
Date: 28 Dec 92 21:26:28 GMT
Distribution: bionet
Checksum: 44838 8

LOCUS       DROADHGN12   3481 bp ds-DNA             INV       01-DEC-1992
DEFINITION  D.pseudoobscura Adh gene for alcohol dehydrogenase
ACCESSION   X62192
KEYWORDS    ADH gene; alcohol dehydrogenase.
SOURCE      Drosophila pseudoobscura DNA.
  ORGANISM  Drosophila pseudoobscura
            Eukaryota; Animalia; Metazoa; Arthropoda; Uniramia; Insecta;
            Pterygota; Neoptera; Holometabola; Diptera; Brachycera;
            Cyclorrhapha; Schizophora; Drosophiloidea; Drosophilidae.
REFERENCE   1  (bases 1 to 3481)
  AUTHORS   Schaeffer,S.W. and Miller,E.L.
  TITLE     Estimates of gene flow in Drosophila pseudoobscura determined from
            nucleotide sequence analysis of the alcohol dehydrogenase region
  JOURNAL   Genetics 132, 471-480 (1992)
  STANDARD  full automatic
COMMENT     *source: strain=GB104; *source: isolate=Gundlach Bundschu Winery,
            Sonoma Valley, *source: California; *source: chromosome=IV section
            88;
               From EMBL    entry DPADHGN12;  dated 16-OCT-1992.
FEATURES             Location/Qualifiers
     mRNA            join(40..119,913..1018,1082..1486,1547..1962)
                     /note="adult mRNA (Adh)"
     mRNA            join(880..1018,1082..1486,1547..1962)
                     /note="larval mRNA (Adh)"
     promoter        8..14
                     /note="adult promoter (Adh)"
     prim_transcript 40..1962
                     /note="adult primary transcript (Adh)"
     exon            40..119
                     /note="adult exon 1a"
     intron          120..912
                     /note="adult intron"
     promoter        844..851
                     /note="larval promoter (Adh)"
     prim_transcript 880..1962
                     /note="larval primary transcript (Adh)"
     exon            880..1018
                     /note="larval exon 1"
     exon            913..1018
                     /note="adult exon 1b"
     intron          1019..1081
                     /number=1
     exon            1082..1486
                     /number=2
     intron          1487..1546
                     /number=2
     exon            1547..1962
                     /number=3
     polyA_signal    1939..1944
     polyA_site      1962
     CDS             join(926..1018,1082..1486,1547..1813)
                     /EC_number="1.1.1.1"
                     /gene="Adh"
                     /product="alcoholdehydrogenase"
                     /codon_start=1
                     /translation="MSLTNKNVVFVAGLGGIGLDTSRELVKRNLKNLVILDRIDNPAA
                     IAELKAINPKVTITFYPYDVTVPVAETTKLLKTIFAQVKTIDVLINGAGILDDHQIER
                     TIAVNYTGLVNTTTAILDFWDKRKGGPGGIICNIGSVTGFNAIYQVPVYSGSKAAVVN
                     FTSSLAKLAPITGVTAYTVNPGITKTTLVHKFNSWLDVEPRVAEKLLEHPTQTSQQCA
                     ENFVKAIELNKNGAIWKLDLGTLEPITWTQHWDSGI"
BASE COUNT      971 a    793 c    797 g    920 t
ORIGIN      
        1 cgtagtgtat ttaaggcact gcacatgtcg aggaccacga ttattgtcta cagtgcagtc
       61 agtagttgtc aggtgcagca ggcggaccag catttcttta aatctactca atcgatcaag
      121 taagtaactg taatccaagt acagtcggcc catcccactc ggttctgccg taactcaaga
      181 aatttcttga tagcgcagtt ccagttgtaa aacgtcgttt aaaaaaacat ctcgttggag
      241 cttgggttga aaaacgttcg agttgggcgt agaaaagttc aaatataaaa aatcaaaaaa
      301 gagaaaatat cttcccaaag cagctgtcca ttttttgtta aagtgataga tcaaaaattc
      361 gtatgacgta tttgccaagt gctctttatg caaataattt cctcaattct aattctacaa
      421 gaagcacttt tggctcttgt ccagaaaaaa caatttgttt acacttgtcg gttgataaca
      481 gaggcggacc gacggctaag taacataaca gtcgcctgtt aagtaatgcc ctcactctct
      541 gttagatggc cgcgcgtgtc gactccgata gaccagcaga cacgcagtag ttttcctcaa
      601 gaacctgaac tctaaacata gacataattt acgatacctt gagactcaaa taaaaataca
      661 agtatgagtg gtattctatt cctgattaga ctcgcacaca cacgtacgag tattaacata
      721 aatttgccgg caaataacca aaacaaaacg aacgcaaacg atccgaacac gtaatgcgag
      781 agataagaaa cagaaaagct cgacgtgaaa gaagcttctg gcggaagctc ttgccgaact
      841 ccctataaat acgagacaga aaaccagaat aaatataata acaaactgtt gaattgtccc
      901 atcgattccc agatcaaaag acagaatgtc tctcaccaac aagaacgtcg ttttcgtggc
      961 cggtctgggc ggcattggcc tggacaccag ccgggagttg gtcaagcgta atctgaaggt
     1021 aagagagaaa gaattccatg gaatctatgg aatcctatat ttaaaaattc atttatttta
     1081 gaacctggtc atcctggatc gcattgacaa tccggctgcc attgccgaac tgaaggcaat
     1141 caatcccaag gtgaccatca ccttctatcc ctacgatgtg actgtgcccg tcgctgagac
     1201 caccaagctc ctgaagacca tctttgccca ggtgaagacc atcgatgtcc tgatcaacgg
     1261 tgctggcatc ctggacgatc atcagatcga gcgcaccatt gccgttaact acacgggcct
     1321 ggtcaacacc accacagcca ttctggactt ctgggacaag cgcaagggcg gcccaggagg
     1381 catcatttgc aacattggct ccgtcaccgg tttcaatgcc atctaccagg tgcccgttta
     1441 ctccggctcc aaggcggcgg tggttaactt tacctcctcc ctggcggtaa gtaatccaac
     1501 gtttttccta ctccccaaaa ctaaatattt ctttgaaatc ccatagaaac tggctcccat
     1561 tactggtgtc actgcttaca ctgtcaatcc tggcatcact aagaccactc tggtccacaa
     1621 attcaactcg tggctggatg tggagccccg tgtggcagag aagctgctcg agcatcccac
     1681 ccagacctct cagcagtgcg ccgagaactt tgtgaaggcc attgagctga acaagaacgg
     1741 tgccatctgg aagttggatt tgggcacctt ggagcccatc acatggaccc agcactggga
     1801 ctcgggcatc taaaccatcc cagagactct atgggacatg gcgttttagc ttttagtttc
     1861 tttttttcca ctcaattgtt acgtatatac atacacatat ggcaataagg ctgatttgaa
     1921 ccccgttttg aatatgataa tacaaaatta tatttgagaa atttcaacaa aatcgataag
     1981 agctatagct ctcttttaat taaaaaaaac tagataaaaa gagcaatggt tgtcagtggt
     2041 ggtcagtggt cgttgtggcc tctatcgatt tccacacaaa aactttactg tttagtaata
     2101 gaaaagaact tgagaaaggc agccaaaaga atgtacgatc tgacgggtaa gcatgtctgc
     2161 tatgtggctg actgcggtgg cattgcactg gagaccagca aggttctcat gaccaagaat
     2221 atagcggtga gtggtagagt ggtgtagaga gtggagagtg gttctggttc ttaaacaagt
     2281 ctctagtctc tagtctctag tctctagtct cttggctgct gctggtagct gtcgaaacta
     2341 attaagttaa tgactttttc atgttttgtt tttgtccgct gacttttgct gttcaaagct
     2401 acaattaagt ttatgttttg agtttgatct acttttgctt aagggaagta ctttcgatta
     2461 cggattgctg gcatataaaa atagatacca aggaatgtgc ctcatatcat attgattcca
     2521 tcttgattct ttgcccagaa actggccatc cttcagagcg tggaaaaccc cccggccatt
     2581 gctcagttac aatcgattaa gcacagcaca cagatcttct tctggacctt cgatgtgacc
     2641 atggcccgcg aggagatgaa gaagtacttc gatgaggtca tggtccagat ggactacatc
     2701 gatgtactga tcaatggggc caccctgtgc gatgagcgga acattgatgc caccatcaat
     2761 acgaatctga ccggcatgat gaacaccgtg gccactgtgc tgccctatat ggaccgtaag
     2821 atgggcggtt cgggtggact gattgtgaat gtcacctccg tcataggact ggatccatcg
     2881 ccggtctttt gtgcgtacag tgcctcaaag tttggtgtga ttgggttcac ccgaagtcta
     2941 gcggtgagtg ggcgagatct taacttgaaa atgctctcta atgtcttcca ttctttcgca
     3001 ggatcccctg tattacaccc aaaatggtgt ggctgtcatg gccgtctgct gtggccccac
     3061 caaagtgttt gtcgatcggg aactgactgc cttcctggaa tacggccagt cctttgccga
     3121 tcgcctgcgt cgtgccccct gccaatcgac ggccgtctgc ggccagaata tagtaaatgc
     3181 catcgagaga tcggagaacg gacagatctg gattgccgac aagggcggcc tggagtcggt
     3241 ggccctccac tggtattggc atatggccga tcagtttgta aactatatgc agagcactga
     3301 cgatgaggat caggagtttt ttttgggcca gcgatgaggc atgccttgat ccacggaata
     3361 aaacatcaaa tgtgtcacaa aaagtaggca acttgtttgt gggtctggaa atagatttga
     3421 aatattttga ggtcatatga ataatgacat aaatgagggt gcctgaaagt atgccaagtt
     3481 t
//