From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Bacillus thuriengiensis delta-endotoxin (CryVB) gene, 5' end.
Date: 22 Dec 92 22:50:26 GMT
Distribution: bionet
Checksum: 16829 8

LOCUS       BACCRYVB     3771 bp ds-DNA             BCT       30-NOV-1992
DEFINITION  Bacillus thuriengiensis delta-endotoxin (CryVB) gene, 5' end.
ACCESSION   L07027
KEYWORDS    delta-endotoxin.
SOURCE      Bacillus thuringiensis (individual_isolate PS33F2) DNA.
  ORGANISM  Bacillus thuringiensis
            Prokaryotae; Firmicutes; Endospore-forming rods and cocci;
            Bacillaceae.
REFERENCE   1  (bases 1 to 3771)
  AUTHORS   Narva,K.E., Payne,J.M., Schwab,G.E., Hickle,L.A., Galasan,T. and
            Sick,A.J.
  TITLE     Novel Bacillus thuringiensis microbes active against nematodes, and
            genes encoding novel nematode-active toxins cloned from Bacillus
            thuringiensis isolates
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     CDS             1..>3771
                     /gene="CryVB"
                     /product="delta-endotoxin"
                     /codon_start=1
                     /translation="MATLNEVYPVNYNVLSSDAFQQLDTTGFKSKYDEMIKAFEKKWK
                     KGAKGKDLLDVAWTYITTGEIDPLNVIKGVLSVLTLIPEVGTVASAASTIVSFIWPKI
                     FGDKPNAKNIFEELKPQIEALIQQDITNYQDAINQKKFDSLQKTINLYTVAIDNNDYV
                     TAKTQLENLNSILTSDISIFIPEGYETGGLPYYAMVANAHILLLRDAIVNAEKLGFSD
                     KEVDTHKKYIKMTIHNHTEAVIKAFLNGLDKFKSLDVNSYNKKANYIKGMTEMVLDLV
                     ALWPTFDPDHYQKEVEIEFTRTISSPIYQPVPKNMQNTSSSIVPSDLFHYQGDLVKLE
                     FSTRTDNDGLAKIFTGIRNTFYKSPNTHETYHVDFSYNTQSSGNISRGSSNPIPIDLN
                     NPIISTCIRNSFYKAIAGSSVLVNFKDGTQGYAFAQAPTGGAWDHSFIESDGAPEGHK
                     LNYIYTSPGDTLRDFINVYTLISTPTINELSTEKIKGFPAEKGYIKNQGIMKYYGKPE
                     YINGAQPVNLENQQTLIFEFHASKTAQYTIRIRYASTQGTKGYFRLDNQELQTLNIPT
                     SHNGYVTGNIGENYDLYTIGSYTITEGNHTLQIQHNDKNGMVLDRIEFVPKDSLQDSP
                     QDSPPEVHESTIIFDKSSPTIWSSNKHSYSHIHLEGSYTSQGSYPHNLLINLFHPTDP
                     NRNHTIHVNNGDMNVDYGKDSVADGLNFNKITATIPSDAWYSGTITSMHLFNDNNFKT
                     ITPKFELSNELENITTQVNALFASSAQDTLASNVSDYWIEQVVMKVDALSDEVFGKEK
                     KALRKLVNQAKRLSKIRNLLIGGNFDNLVAWYMGKDVVKESDHELFKSDHVLLPPPTF
                     HPSYIFQKVEESKLKPNTRYTISGFIAHGEDVELVVSRYGQEIQKVMQVPYEEALPLT
                     SESNSSCCVPNLNINETLADPHFFSYSIDVGSLEMEANPGIEFGLRIVKPTGMARVSN
                     LEIREDRPLTAKEIRQVQRAARDWKQNYEQERTEITAIIQPVLNQINALYENEDWNGS
                     IRSNVSYHDLEQIMLPTLLKTEEINCNYDHPAFLLKVYHWFMTDRIGEHGTILARFQE
                     ALDRAYTQLESRNLLHNGHFTTDTANWTIEGDAHHTILEDGRRVLRLPDWSSNATQTI
                     EIEDFDLDQEYQLLIHAKGKGSITLQHGEENEYVETHTHHTNDFITSQNIPFTFKGNQ
                     IEVHITSEDGEFLIDHITVIEVSKTDTNTNIIENSPINTSMNSNVRVDIPRSL"
BASE COUNT     1417 a    614 c    591 g   1149 t
ORIGIN      
        1 atggctacac ttaatgaagt atatcctgtg aattataatg tattatcttc tgatgctttt
       61 caacaattag atacaacagg ttttaaaagt aaatatgatg aaatgataaa agcattcgaa
      121 aaaaaatgga aaaaaggggc aaaaggaaaa gaccttttag atgttgcatg gacttatata
      181 actacaggag aaattgaccc tttaaatgta attaaaggtg ttttatctgt attaacttta
      241 attcctgaag ttggtactgt ggcctctgca gcaagtacta ttgtaagttt tatttggcct
      301 aaaatatttg gagataaacc aaatgcaaaa aatatatttg aagagctcaa gcctcaaatt
      361 gaagcattaa ttcaacaaga tataacaaac tatcaagatg caattaatca aaaaaaattt
      421 gacagtcttc agaaaacaat taatctatat acagtagcta tagataacaa tgattacgta
      481 acagcaaaaa cgcaactcga aaatctaaat tctatactta cctcagatat ctccatattt
      541 attccagaag gatatgaaac tggaggttta ccttattatg ctatggttgc taatgctcat
      601 atattattgt taagagacgc tatagttaat gcagagaaat taggctttag tgataaagaa
      661 gtagacacac ataaaaaata tatcaaaatg acaatacaca atcatactga agcagtaata
      721 aaagcattct taaatggact tgacaaattt aagagtttag atgtaaatag ctataataaa
      781 aaagcaaatt atattaaagg tatgacagaa atggttcttg atctagttgc tctatggcca
      841 actttcgatc cagatcatta tcaaaaagaa gtagaaattg aatttacaag aactatttct
      901 tctccaattt accaacctgt acctaaaaac atgcaaaata cctctagctc tattgtacct
      961 agcgatctat ttcactatca aggagatctt gtaaaattag aattttctac aagaacggac
     1021 aacgatggtc ttgcaaaaat ttttactggt attcgaaaca cattctacaa atcgcctaat
     1081 actcatgaaa cataccatgt agattttagt tataataccc aatctagtgg taatatttca
     1141 agaggctctt caaatccgat tccaattgat cttaataatc ccattatttc aacttgtatt
     1201 agaaattcat tttataaggc aatagcggga tcttctgttt tagttaattt taaagatggc
     1261 actcaagggt atgcatttgc ccaagcacca acaggaggtg cctgggacca ttcttttatt
     1321 gaatctgatg gtgccccaga agggcataaa ttaaactata tttatacttc tccaggtgat
     1381 acattaagag atttcatcaa tgtatatact cttataagta ctccaactat aaatgaacta
     1441 tcaacagaaa aaatcaaagg ctttcctgcg gaaaaaggat atatcaaaaa tcaagggatc
     1501 atgaaatatt acggtaaacc agaatatatt aatggagctc aaccagttaa tctggaaaac
     1561 cagcaaacat taatattcga atttcatgct tcaaaaacag ctcaatatac cattcgtata
     1621 cgttatgcca gtacccaagg aacaaaaggt tattttcgtt tagataatca ggaactgcaa
     1681 acgcttaata tacctacttc acacaacggt tatgtaaccg gtaatattgg tgaaaattat
     1741 gatttatata caataggttc atatacaatt acagaaggta accatactct tcaaatccaa
     1801 cataatgata aaaatggaat ggttttagat cgtattgaat ttgttcctaa agattcactt
     1861 caagattcac ctcaagattc acctccagaa gttcacgaat caacaattat ttttgataaa
     1921 tcatctccaa ctatatggtc ttctaacaaa cactcatata gccatataca tttagaagga
     1981 tcatatacaa gtcagggaag ttatccacac aatttattaa ttaatttatt tcatcctaca
     2041 gaccctaaca gaaatcatac tattcatgtt aacaatggtg atatgaatgt tgattatgga
     2101 aaagattctg tagccgatgg gttaaatttt aataaaataa ctgctacgat accaagtgat
     2161 gcttggtata gcggtactat tacttctatg cacttattta atgataataa ttttaaaaca
     2221 ataactccta aatttgaact ttctaatgaa ttagaaaaca tcacaactca agtaaatgct
     2281 ttattcgcat ctagtgcaca agatactctc gcaagtaatg taagtgatta ctggattgaa
     2341 caggtcgtta tgaaagtcga tgccttatca gatgaagtat ttggaaaaga gaaaaaagca
     2401 ttacgtaaat tggtaaatca agcaaaacgt ctcagtaaaa tacgaaatct tctcataggt
     2461 ggtaattttg acaatttagt cgcttggtat atgggaaaag atgtagtaaa agaatcggat
     2521 catgaattat ttaaaagtga tcatgtctta ctacctcccc caacattcca tccttcttat
     2581 attttccaaa aggtggaaga atcaaaacta aaaccaaata cacgttatac tatttctggt
     2641 tttatcgcac atggagaaga tgtagagctt gttgtctctc gttatgggca agaaatacaa
     2701 aaagtgatgc aagtgccata tgaagaagca cttcctctta catctgaatc taattctagt
     2761 tgttgtgttc caaatttaaa tataaatgaa acactagctg atccacattt ctttagttat
     2821 agcatcgatg ttggttctct ggaaatggaa gcgaatcctg gtattgaatt tggtctccgt
     2881 attgtcaaac caacaggtat ggcacgtgta agtaatttag aaattcgaga agaccgtcca
     2941 ttaacagcaa aagaaattcg tcaagtacaa cgtgcagcaa gagattggaa acaaaactat
     3001 gaacaagaac gaacagagat cacagctata attcaacctg ttcttaatca aattaatgcg
     3061 ttatacgaaa atgaagattg gaatggttct attcgttcaa atgtttccta tcatgatcta
     3121 gagcaaatta tgcttcctac tttattaaaa actgaggaaa taaattgtaa ttatgatcat
     3181 ccagcttttt tattaaaagt atatcattgg tttatgacag atcgtatagg agaacatggt
     3241 actattttag cacgtttcca agaagcatta gatcgtgcat atacacaatt agaaagtcgt
     3301 aatctcctgc ataacggtca ttttacaact gatacagcga attggacaat agaaggagat
     3361 gcccatcata caatcttaga agatggtaga cgtgtgttac gtttaccaga ttggtcttct
     3421 aatgcaactc aaacaattga aattgaagat tttgacttag atcaagaata ccaattgctc
     3481 attcatgcaa aaggaaaagg ttccattact ttacaacatg gagaagaaaa cgaatatgtg
     3541 gaaacacata ctcatcatac aaatgatttt ataacatccc aaaatattcc tttcactttt
     3601 aaaggaaatc aaattgaagt ccatattact tcagaagatg gagagttttt aatcgatcac
     3661 attacagtaa tagaagtttc taaaacagac acaaatacaa atattattga aaattcacca
     3721 atcaatacaa gtatgaatag taatgtaaga gtagatatac caagaagtct c
//