From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Bacillus subtilis heat shock protein (groEL and groES) genes,
Date: 24 Jul 92 10:05:11 GMT
Distribution: bionet
Checksum: 52605     6

LOCUS       BACGROESL    2400 bp ds-DNA             BCT       24-JUL-1992
DEFINITION  Bacillus subtilis heat shock protein (groEL and groES) genes,
            complete cds.
ACCESSION   M81132
KEYWORDS    groEL gene; groES gene; heat shock protein.
SOURCE      Bacillus subtilis (strain 168) DNA.
  ORGANISM  Bacillus subtilis
            Prokaryotae; Firmicutes; Endospore-forming rods and cocci;
            Bacillaceae.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Li,M. and Wong,S.-L.
  TITLE     Cloning and characterization of the groESL operon from Bacillus
            subtilis
  JOURNAL   J. Bacteriol. 174, 3981-3992 (1992)
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     promoter        253..289
                     /evidence=EXPERIMENTAL
                     /citation=[1]
     -35_signal      253..259
                     /evidence=EXPERIMENTAL
                     /citation=[1]
     -10_signal      276..282
                     /evidence=EXPERIMENTAL
                     /citation=[1]
     RBS             340..346
                     /gene="groES"
                     /note="putative"
     CDS             354..638
                     /function="Molecular Chaperone"
                     /standard_name="GroES (cpn10)"
                     /product="heat shock protein"
                     /gene="groES"
                     /evidence=EXPERIMENTAL
                     /codon_start=1
                     /translation="MLKPLGDRVVIELVESEEKTASGIVLPDSAKEKPQEGKIVAAGS
                     GRVLESGERVALEVKEGDRIIFSKYAGTEVKYEGTEYLILRESDILAVIG"
                     /citation=[1]
     RBS             669..675
                     /gene="groEL"
                     /evidence=EXPERIMENTAL
                     /citation=[1]
     CDS             685..2319
                     /function="Molecular Chaperone"
                     /standard_name="GroEL (cpn60)"
                     /product="heat shock protein"
                     /gene="groEL"
                     /codon_start=1
                     /translation="MAKEIKFSEEARRAMLRGVDALADAVKVTLGPKGRNVVLEKKFG
                     SPLITNDGVTIAKEIELEDAFENMGAKLVAEVASKTNDVAGDGTTTATVLAQAMIREG
                     LKNVTAGANPVGVRKGMEQAVAVAIENLKEISKPIEGKESIAQVAAISAADEEVGSLI
                     AEAMERVGNDGVITIEESKGFTTELEVVEGMQFDRGYASPYMVTDSDKMEAVLDNPYI
                     LITDKKITNIQEILPVLEQVVQQGKPLLLIAEDVEGEALATLVVNKLRGTFNAVAVKA
                     PGFGDRRKAMLEDIAVLTGGEVITEDLGLDLKSTQIAQLGRASKVVVTKENTTIVEGA
                     GETDKISARVTQIRAQVEETTSEFDREKLQERLAKLAGGVAVIKVGAATETELKERKL
                     RIEDALNSTRAAVEEGIVSGGGTALVNVYNKVAAVEAEGDAQTGINIVLRALEEPIRQ
                     IAHNAGLEGSVIVERLKNEEIGVGFNAATGEWVNMIEKGIVDPTKVTRSALQNAASVA
                     AMFLTTEAVVADKPEENGGGAGMPDMGGMGGMGGMM"
                     /citation=[1]
BASE COUNT      757 a    475 c    584 g    584 t
ORIGIN
        1 catctgtagg ctgtcctccg gcataaccga atttaaataa caagggaatc gcgattaatg
       61 cggaaaactg catgatgata tatgtcaaaa taataaacca atactgtttt ctcaaatggt
      121 atgtactcct ttgttaagtg ggtttcgttc atctacagct attgtaacat aatcggtacg
      181 ggggtgaaaa agctaacgga aaagggagcg gaaaagaatg atgtaagcgt gaaaaatttt
      241 ttatcttatc acttgaaatt ggaagggaga ttctttatta taagaattgt gttagcactc
      301 tttagtgctg agtgctaaaa ttacatattc atactattga ggaggttatt tcattgttaa
      361 agccattagg tgatcgcgtt gtcattgaac tcgtagaatc tgaagaaaaa actgccagcg
      421 gaattgtgtt gccggattct gcaaaagaaa aaccgcaaga aggcaaaatc gtggcagctg
      481 gttcaggtcg cgtgttagaa agcggagagc gcgttgcttt agaagtaaaa gagggcgacc
      541 gcattatctt ctcaaaatac gcaggtactg aagtgaaata cgaaggtact gaatacttaa
      601 tcttacgtga aagcgacatt ttagctgtta tcggctaatt cttaaataaa caatacttaa
      661 aacatttgag gaggtcttgt aaacatggca aaagaaatta agtttagtga agaagctcgc
      721 cgcgcaatgc ttcgcggtgt cgatgcactt gctgatgctg ttaaagtaac tttaggacca
      781 aaaggacgca acgtggttct agagaaaaaa ttcggttctc cgttaatcac aaatgacggt
      841 gtaacaatcg ctaaagaaat cgagctagaa gacgcgtttg aaaacatggg tgctaagctt
      901 gttgctgaag tagccagcaa aacaaacgac gttgccggtg acggtacaac aactgcaaca
      961 gttcttgcgc aagcaatgat ccgtgaaggc cttaaaaacg taacagcagg cgctaaccct
     1021 gtaggcgtgc gtaaagggat ggaacaagct gtagcggttg cgatcgaaaa cttaaaagaa
     1081 atttctaagc caatcgaagg caaagagtct atcgctcagg ttgctgcgat ctctgctgct
     1141 gatgaggaag tcggaagcct tatcgctgaa gcaatggagc gcgtaggaaa cgacggcgtt
     1201 atcacaatcg aagagtctaa aggcttcaca actgagcttg aagttgttga aggtatgcaa
     1261 ttcgaccgcg gatatgcgtc tccttacatg gtaactgact ctgataagat ggaagcggtt
     1321 cttgacaatc cttacatctt aatcacagac aaaaaaatca caaacattca agaaatcctt
     1381 cctgtgcttg agcaggttgt tcagcaaggc aaaccattgc ttctgatcgc tgaggatgtt
     1441 gaaggcgaag cacttgctac acttgttgtg aacaaacttc gcggcacatt caacgcagtg
     1501 gctgttaaag ctcctggttt cggtgatcgc cgtaaagcaa tgcttgaaga catcgctgtc
     1561 cttactggcg gagaagtcat cacagaagat cttggccttg acctgaaatc tactcaaatc
     1621 gctcaattgg gacgcgcttc taaagttgtc gttactaaag aaaacacaac aatcgttgaa
     1681 ggcgctggcg aaacagacaa aatttctgcc cgtgtgactc aaatccgcgc tcaagtggaa
     1741 gaaacaactt ctgaattcga cagagaaaaa ttacaagagc gtcttgctaa acttgctggc
     1801 ggcgtagctg tcatcaaagt cggcgctgcg actgaaactg agctgaaaga gcgtaaactt
     1861 cgcatcgaag acgccttgaa ctcaactcgc gcagctgttg aagaaggcat cgtatccggt
     1921 ggtggtacag cgcttgtaaa cgtatataac aaagtcgctg cagttgaagc tgaaggcgat
     1981 gctcaaacag gtatcaacat cgtgcttcgc gcgcttgaag agccaatccg tcaaatcgca
     2041 cacaacgctg gccttgaagg atctgtcatc gttgagcgcc tcaaaaacga agaaatcggc
     2101 gtaggcttca acgctgcaac tggcgaatgg gtaaacatga tcgaaaaagg tatcgttgac
     2161 ccaacaaaag ttacacgctc agctcttcaa aacgctgcgt ctgtagctgc aatgttctta
     2221 acaacagaag ctgttgtcgc tgacaagcca gaagaaaacg gtggcggcgc aggaatgcct
     2281 gatatgggcg gcatgggcgg tatgggtgga atgatgtaat aaagggatat taacccttat
     2341 acatcaatgt tttaaccgtc ttaaaaaact agacaaagcg tgaataaaaa aagagaaggt
//