From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Acanthamoeba castellanii mitochondrial Met-tRNA, Ala-tRNA,
Date: 16 Dec 92 23:23:06 GMT
Distribution: bionet
Checksum: 24834 4

LOCUS       ACAMTTG       634 bp ds-DNA             ORG       15-DEC-1992
DEFINITION  Acanthamoeba castellanii mitochondrial Met-tRNA, Ala-tRNA,
            Pro-tRNA, and Asp-tRNA genes.
ACCESSION   M97651
KEYWORDS    transfer RNA-Ala; transfer RNA-Asp; transfer RNA-Met;
            transfer RNA-Pro.
SOURCE      Mitochondrion Acanthamoeba castellanii (strain Neff, chromosome
            Mitochondrion Acanthamoeba castellanii) (library: ATCC 30010) DNA.
  ORGANISM  Mitochondrion Acanthamoeba castellanii
            Eukaryota; Animalia; Protozoa; Sarcomastigophora; Sarcodina;
            Rhizopoda; Lobosa; Gymnamoeba; Amoebida; Acanthopodina;
            Acanthamoebidae.
REFERENCE   1  (bases 1 to 634)
  AUTHORS   Lonergan,K.M. and Gray,M.W.
  TITLE     Editing of transfer RNAs in Acanthamoeba castellanii mitochondria
  JOURNAL   Science (1993) In press
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     tRNA            34..104
                     /product="transfer RNA-Met"
                     /gene="Met-tRNA"
                     /evidence="EXPERIMENTAL"
                     /citation=[1]
                     /note="Post-transcriptional RNA editing changes position
                     35 from Ato G and position 36 from U to G.; anticodon
                     'cau'"
     tRNA            135..205
                     /product="transfer RNA-Ala"
                     /gene="Ala-tRNA"
                     /evidence="EXPERIMENTAL"
                     /citation=[1]
                     /note="Post-transcriptional RNA editing changes position
                     137 from Uto A.; anticodon 'tgc'"
     tRNA            213..285
                     /product="transfer RNA-Pro"
                     /gene="Pro-tRNA"
                     /evidence="EXPERIMENTAL"
                     /citation=[1]
                     /note="anticodon 'ugg'"
     tRNA            289..361
                     /product="transfer RNA-Asp"
                     /gene="Asp-tRNA"
                     /evidence="EXPERIMENTAL"
                     /citation=[1]
                     /note="Post-transcriptional RNA editing changes position
                     290 from Uto A and position 291 from U to G. The
                     5'-terminus(position 289) is also probably edited from U
                     to G.; anticodon 'ugg'"
     tRNA            498..569
                     /product="transfer RNA-Met"
                     /gene="Met-tRNA"
                     /evidence="EXPERIMENTAL"
                     /label=e
                     /citation=[1]
                     /note="Post-transcriptional RNA editing changes position
                     499 from Uto A.; anticodon 'cau'"
BASE COUNT      188 a     90 c    114 g    242 t
ORIGIN      
        1 ttaaaaaaaa atatcgaggg taaaaaatcc gataatccta tagtttaatg gtaaaatatt
       61 tcgctcataa cgaaaggtta ttggttcgat tccagttagg cctattttat tagggagatt
      121 gtaattagcc gcttggttgc atagtttaat ggtaaaatca ataccttgca cgtattagat
      181 atcagttcga ttctgattgc gtccactttg tgcagagtgt agcataatag gtaatgtgtc
      241 tgttttggga acagaagact ataggttcga gtcctgtcac tctgagcgtt tagtgtaact
      301 taattggtaa agagttaact tgtcgagtta aaagatgcag gttcgagtcc tgtcactctc
      361 actacttaga tttttaaatt accataaatt acttaaacta gtattttttt gaaaactgcc
      421 ttcttatatc ctatatattt cagctgtctt tttttttttt atataaaggt tacttaaatt
      481 tgttttttta ttggcttttg gaggtattgt aaatggaaac atatcaggct catgccctga
      541 cgatgtaggt tcgagtccta ccctcctgat ttttggtaaa gttatattta gtaaatttca
      601 aagatattat attatatata agtaattcca ttac
//