HDTenEightyP/NetNews huggingface dataset:
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Thylacinus cyncocephalus cytochrome b gene, partial cds.
Date: 20 Aug 92 10:05:00 GMT
Distribution: bionet
Checksum: 56247     3

LOCUS       THNCYTOB      642 bp ds-DNA             ORG       20-AUG-1992
DEFINITION  Thylacinus cyncocephalus cytochrome b gene, partial cds.
ACCESSION   M99452
KEYWORDS    cytochrome b.
SOURCE      Mitochondrion Thylacinus cyncocephalus (chromosome Mitochondrion
            Thylacinus cyncocephalus) (library: USNM 49723) DNA.
  ORGANISM  Mitochondrion Thylacinus cyncocephalus
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Metatheria; Marsupialia; Marsupicarnivora; Dasyuridae;
            Thylacinidae.
REFERENCE   1  (bases 1 to 642)
  AUTHORS   Krajewski,C., Driskell,A.C., Baverstock,P.R. and Braun,M.J.
  TITLE     Phylogenetic relationships of the thylacine (Marsupialia:
            Thylacinidae) among dasyuroid marsupials: evidence from
            cytochrome-b DNA sequences
  JOURNAL   Proc. R. Soc. Lond., B, Biol. Sci. (1992) In press
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     CDS             1..642
                     /product="cytochrome b"
                     /note="putative"
                     /codon_start=2
                     /translation="FGSLLGICLVIQILTGLFLAMHYTSDTSTAFSSVAHICRDVNYG
                     WLIRNLHANGASMFFMCLFLHVGRGIYYGSYLYKETWNIGVILLLTVMATAFVGYVLP
                     WGQMSFWGATVITNLLSAIPYIGTTLAEWVWGGFAVDKATLTRFFAFHFILPSIVTAR
                     ATVHLLFLHETGSNNPSGINPDSDKIPFHPYXSIKDTLGLMLLLLPLLPLALFS"
                     /citation=[1]
                     /partial
BASE COUNT      180 a    182 c     91 g    184 t      5 others
ORIGIN
        1 cttcgggtcc ttactaggaa tctgcctagt cattcaaatc ttaacaggcc tatttctagc
       61 aatacattat acatcagaca catcaactgc cttctcctca gtagcacata tctgccgaga
      121 cgtaaattat ggatgactta ttcgtaacct ccatgccaat ggagcctcca tattcttcat
      181 atgcttattt cttcatgtag gacgaggtat ctactacgga tcatacctgt acaaagaaac
      241 atgaaacatt ggagttatcc tcctactaac agtaatagca actgcattcg taggatatgt
      301 ccttccatga ggccaaatat cattctgagg tgctaccgtc attactaacc tactatctgc
      361 catcccttac attggaacta ctttagcaga atgagtttga ggaggattcg cagtggacaa
      421 agcaacacta acacgattct ttgccttcca ctttatccta ccctccattg taacagcacg
      481 agctactgtt cacctactat tccttcatga aacaggctct aataacccct caggaattaa
      541 cccagactca gacaaaatcc cattccatcc ctactywtcc atcaaagata ccctaggcct
      601 catactcctc ctncttccac tcctncccct agcyctattc tc
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: 1E9-VK
Date: 13 Aug 92 10:00:50 GMT
Distribution: bionet
Checksum: 62930     3

LOCUS       MUSD12729     334 bp ss-mRNA            ROD       13-AUG-1992
DEFINITION  1E9-VK
ACCESSION   D12729
KEYWORDS    .
SOURCE      Mus musculus (strain BALB/c) mRNA.
  ORGANISM  Mus musculus
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Rodentia; Myomorpha; Muridae; Murinae.
REFERENCE   1  (bases 1 to 334)
  AUTHORS   Sawada,J., Mizusawa,S., Terao,T., Naito,M. and Kurosawa,Y.
  TITLE     Molecular characterization of monoclonal anti-steroid antibodies:
            primary structures of the variable regions of seven antibodies
            specific for 17alpha-hydroxyprogesterone or 11-deoxycortisol and
            their pH-reactivity profiles
  JOURNAL   Mol. Immunol. 28, 1063-1072 (1991)
  STANDARD  full automatic
COMMENT     Submitted (27-JUL-1992) to DDBJ by:
            Jun-ichi Sawada
            Dept. of Biochem. and Immunochem.
            National Institute of Hygienic Sciences
            1-18-1 Kamiyoga
            Setagaya, Tokyo 158
            Japan
            Phone:  03-3700-1141 x240
            Fax:    03-3707-6950
FEATURES             Location/Qualifiers
     CDS             <1..>334
                     /product="'Ig kappa chain precursor'"
                     /codon_start=1
                     /translation="NIVLTQSPASLAVSLGQRAIISCRTSESVDRYGRSFMHWYQQKS
                     GQPPKLLIYLATNLESGVPARFSGSGSRTDFTLTIESVEADDAATYYCQQNYEDPYPF
                     GGGTKLEIK"
     mat_peptide     1..>334
                     /note="'VJ region (1-334)'"
                     /product="'Ig kappa chain'"
                     /codon_start=1
BASE COUNT       87 a     84 c     83 g     80 t
ORIGIN
        1 aacattgtgc tgacccaatc tccagcttct ttggctgtgt ctctagggca gagggccatc
       61 atatcctgta gaaccagtga aagtgttgac aggtatggca gaagttttat gcactggtac
      121 cagcagaagt caggacagcc acccaaactc ctcatctatc ttgcaaccaa ccttgaatct
      181 ggggtccctg ccaggttcag tggcagtggc tctaggacag acttcaccct caccattgaa
      241 tctgtggagg ctgatgatgc tgcaacctat tactgtcagc aaaattatga ggatccgtac
      301 ccgttcggag gggggaccaa gctggaaata aaac
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Rotavirus outer capsid protein VP7 RNA, complete cds.
Date: 13 Aug 92 10:01:11 GMT
Distribution: bionet
Checksum: 34452     3

LOCUS       ROTVP7AA     1062 bp ds-RNA             VRL       13-AUG-1992
DEFINITION  Rotavirus outer capsid protein VP7 RNA, complete cds.
ACCESSION   M93006
KEYWORDS    outer capsid protein VP7.
SOURCE      Rotavirus cDNA to genomic RNA.
  ORGANISM  Rotavirus
            Viridae; ds-RNA nonenveloped viruses; Reoviridae.
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Palombo,E.A., Bishop,R.F. and Cotton,R.G.
  TITLE     Intra- and inter- season genetic variability in the VP7 gene of
            serotype 1 (monotype 1A) rotavirus clinical isolates
  JOURNAL   Unpublished (1991)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     CDS             49..1029
                     /function="virus neutralization"
                     /standard_name="VP7"
                     /product="outer capsid protein VP7"
                     /gene="outer capsid protein VP7"
                     /note="putative"
                     /codon_start=1
                     /translation="MYGIEYTTILIFLISIILLNYILKSVTRIMDYIIYRSLLIYVAL
                     FALTRAQNYGLNLPITGSMDTVYANSTQEGIFLTSTLCLYYPTEASTQINDGEWKDSL
                     SQMFLTKGWPTGSVYFKEYSNIVDFSVDPQLYCDYNLVLMKYDQNLELDMSELADLIL
                     NEWLCNPMDITLYYYQQSGESNKWISMGSSCTVKVCPLNTQTLGIGCQTTNVDSFEMV
                     AENEKLAIVDVVDGINHKINFTTTTCTIRNCKKLGPRENVAVIQVGGSNVLDITADPT
                     TNPQTERMMRVNWKKWWQVFYTIVDYINQIIQVMSKRSRSLNSAAFYYRV"
                     /citation=[1]
BASE COUNT      381 a    149 c    191 g    341 t
ORIGIN
        1 ggctttaaaa gagagaattt ccgtctggct aacggttagc tccttttaat gtatggtatt
       61 gaatatacca caattctaat ctttctgata tcaattattc tactcaacta tatattaaaa
      121 tcagtaactc gaataatgga ctacattata tatagatctt tgttgattta tgtagcatta
      181 tttgccttga caagagctca gaattatggg cttaacttac caataacagg atcaatggac
      241 actgtatacg ctaactctac tcaagaagga atatttctaa catctacatt atgtttgtat
      301 tatccaactg aagcaagtac tcaaattaat gatggtgaat ggaaagactc attgtcacaa
      361 atgtttctca caaaaggttg gccaacagga tcagtctatt ttaaagagta ttcaaatatt
      421 gttgattttt ctgttgatcc acaattatat tgtgattata acttagtact aatgaaatat
      481 gatcaaaatc ttgaattaga tatgtcagag ttagctgatt taatattgaa cgaatggtta
      541 tgtaatccaa tggatataac attatattat tatcaacaat cgggagaatc aaataagtgg
      601 atatcaatgg gatcatcatg cactgtgaaa gtgtgtccac tgaatacgca aacgttagga
      661 ataggttgtc aaactacaaa tgtagactcg tttgaaatgg ttgctgagaa tgagaaatta
      721 gctatagtgg atgtcgttga tgggataaat cataaaataa atttcacaac tacgacatgt
      781 actattcgaa attgtaagaa gttaggtcca agagagaatg tagctgtaat acaagttggt
      841 ggctctaatg tattagacat aacagcagat ccaacgacta atccacaaac tgagagaatg
      901 atgagagtga attggaaaaa atggtggcaa gtattttaca ctatagtaga ttatattaac
      961 cagatcatac aggtaatgtc caaaagatca agatcattaa attctgcagc tttttactat
     1021 agagtataga tatatcttag attagaattg tatgatgtga cc
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human chromosome 4 sequence-tagged site STS4-108
Date: 21 Aug 92 10:06:37 GMT
Distribution: bionet
Checksum: 02290     3

LOCUS       HUM4STS108    431 bp ds-DNA             PRI       21-AUG-1992
DEFINITION  Human chromosome 4 sequence-tagged site STS4-108
ACCESSION   M95253
KEYWORDS    STS; human chromosome 4; sequence tagged site.
SOURCE      Homo sapiens, clone C4-158 from Los Alamos National Laboratory
            chromosome 4 cosmid library, plate 4-1-2R, pos 8A; vector SuperCos
            1 (Stratagene)
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 431)
  AUTHORS   Goold,R.D., diSibio,G., Dugaiczyk,A., Smith,K.A., Xu,H.,
            Magrane,G., Lang,D.B., Cox,D.R., Masters,S.B. and Myers,R.M.
  TITLE     Sequence-Tagged Sites for Human Chromosome 4
  JOURNAL   Unpublished (1992) see COMMENT for author address
  STANDARD  full automatic
COMMENT     PCR components: 25 ng of human genomic DNA, 10 pmol of each
            oligonucleotide, 200 micro-M dNTPs, 0.25 U Taq  polymerase (Cetus)
            in 10 micro-l of 50 mM KCl-20 mM  Tris-HCl, pH 8.3 (at room temp),
            2.5 mM MgCl-2.  Initial denaturation at 94degC for 1.5 min, then 30
            cycles of  94degC for 15 sec, 62degC for 23 sec, and 72degC for 30
            sec, followed by a final extension at 72degC for 3.5 min, using a
            Perkin-Elmer 9600 thermocycler. PCR-amplified product size 169 bp.
            Sequence submitted by:
            Human Genome Mapping Center
            Box 0925
            University of California San Francisco
            San Francisco, CA 94143-0925  USA
            Phone: (415) 502-1612   Fax: (415) 476-8391
            e-mail: hgmcprobes@cgl.ucsf.edu
FEATURES             Location/Qualifiers
     misc_feature    52..77
                     /label=Primer1
     misc_feature    complement(197..220)
                     /label=Primer2
BASE COUNT       97 a    114 c     80 g    123 t     17 others
ORIGIN
        1 aaatnaaatc ctgctttctt attatcatta tttngtattt gctcaaggaa agctacttaa
       61 agactaagta aagaacagag ttgggagagg taaagtacca acttcttgac cacatgtaag
      121 gagttcttag actggccttc ctgtgtgtat acttaggtct caattcacca ccatgataag
      181 gcctgctttg gcatatactc taaactcagg gatgatctca aaattatatc ttcttttttt
      241 ttttnnggng acggagtctc actctgtcac ccaggctgga gtgcagcngt gtntatctca
      301 gctcactgca agctccccct cccgggttca tgccattctc ctacctcagc ccccccccnt
      361 cngntggcac tacaggcacc ntgccaccac acctggntaa ttncctngtt ctcccgtcgn
      421 gncggcgcct n
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human polymorphic microsatellite DNA.
Date: 19 Aug 92 10:00:59 GMT
Distribution: bionet
Checksum: 08391     2

LOCUS       HUM5PLYSAT    288 bp ds-DNA             PRI       19-AUG-1992
DEFINITION  Human polymorphic microsatellite DNA.
ACCESSION   M99145
KEYWORDS    microsatellite polymorphism.
SOURCE      Homo sapiens DNA.
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 288)
  AUTHORS   Bowcock,A.M.Ph.D.., Osborne-Lawrence,S.L., Barnes,R.I.,
            Chakravarti,A., Washington,S. and Dunn,C.
  TITLE     A linkage map of human chromosome 13q containing microsatellite
            polymorphisms
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     satellite       1..288
                     /note="map=13q; awaiting approval /map qualifier"
BASE COUNT       76 a     80 c     33 g     96 t      3 others
ORIGIN
        1 tgtatggtca ttctcaagac tctactgaca tttgctccat aggaccacag acatcagagt
       61 ccttacaatg ttagtcaaaa gtaatctctc tctgtttctc tctctgtgtc tctgtctatc
      121 tctgtctctt tatttctctc tctccacaca cacacacaca cacacacaca cacatgcacg
      181 cctgctgtca cacaagttat ataatsctgt cccaggtcca aatactattt ctaactgcct
      241 tcatntgatn tattatttca actaagctat ttacaggtat ctaagact
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Odocoileus hemionus columbianus cytochrome b gene, 3' end.
Date: 19 Aug 92 10:03:04 GMT
Distribution: bionet
Checksum: 61129     2

LOCUS       ODOTEHCB      307 bp ds-DNA             ORG       19-AUG-1992
DEFINITION  Odocoileus hemionus columbianus cytochrome b gene, 3' end.
ACCESSION   M98487
KEYWORDS    cytochrome b.
SOURCE      Mitochondrion Odocoileus hemionus (chromosome Mitochondrion
            Odocoileus hemionus, sub_species columbianus) adult liver DNA.
  ORGANISM  Mitochondrion Odocoileus hemionus
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Artiodactyla; Ruminantia; Pecora; Cervidae; Odocoileinae.
REFERENCE   1  (bases 1 to 307)
  AUTHORS   Carr,S.M. and Hughes,G.A.
  TITLE     The direction of hybridization between species of North American
            deer (Odocoileus) as inferred from mitochondrial cytochrome b
            sequences
  JOURNAL   J. Mammal. (1992) In press
  STANDARD  full staff_entry
FEATURES             Location/Qualifiers
     CDS             <1..298
                     /product="cytochrome b"
                     /codon_start=3
                     /translation="FGSLLGICLILQILTGLFLAMHYTSDTMTAFSSVTHICRDVNYG
                     WIIRYMHANGASMFFICLFMHVGRGLYYGSYTFLETWNIGVILLFTVMATAFVG"
BASE COUNT       88 a     73 c     47 g     99 t
ORIGIN
        1 acttcggctc tctgctagga atttgcttaa tcctacaaat tcttactggt ctattcctag
       61 caatacatta tacatccgac acaataacag cattttcctc tgtcactcac atctgccgag
      121 acgttaacta tggctgaatc attcgatata tacatgccaa tggagcatcc atattcttta
      181 tctgtctatt tatacatgtg ggacgaggac tatactacgg atcatatact ttcttagaaa
      241 catgaaacat tggagtaatt ctcctattca cagttatagc cacagcattc gtagggtacg
      301 tcctacc
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human chromosome 4 sequence-tagged site STS4-310
Date: 21 Aug 92 10:08:50 GMT
Distribution: bionet
Checksum: 16989     3

LOCUS       HUM4STS310    442 bp ds-DNA             PRI       21-AUG-1992
DEFINITION  Human chromosome 4 sequence-tagged site STS4-310
ACCESSION   L00791
KEYWORDS    STS; human chromosome 4; sequence tagged site.
SOURCE      Homo sapiens, clone p4-248 from a chromosome 4 plasmid library;
            vector pBluescript II KS + (Stratagene)
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 442)
  AUTHORS   Goold,R.D., diSibio,G., Dugaiczyk,A., Smith,K.A., Xu,H.,
            Magrane,G., Lang,D.B., Cox,D.R., Masters,S.B. and Myers,R.M.
  TITLE     Sequence-Tagged Sites for Human Chromosome 4
  JOURNAL   Unpublished (1992) see COMMENT for author address
  STANDARD  full automatic
COMMENT     PCR components: 25 ng of human genomic DNA, 10 pmol of each
            oligonucleotide, 200 micro-M dNTPs, 0.25 U Taq  polymerase (Cetus)
            in 10 micro-l of 50 mM KCl-20 mM  Tris-HCl, pH 8.3 (at room temp),
            2.5 mM MgCl-2.  Initial denaturation at 94degC for 1.5 min, then 30
            cycles of  94degC for 15 sec, 64degC for 23 sec, and 72degC for 30
            sec, followed by a final extension at 72degC for 3.5 min, using a
            Perkin-Elmer 9600 thermocycler. PCR-amplified product size 251 bp.
            STS sequence contains a simple sequence tandem repeat (CA).
            Sequence submitted by:
            Human Genome Mapping Center
            Box 0925
            University of California San Francisco
            San Francisco, CA 94143-0925  USA
            Phone: (415) 502-1612   Fax: (415) 476-8391
            e-mail: hgmcprobes@cgl.ucsf.edu
FEATURES             Location/Qualifiers
     misc_feature    13..36
                     /label=Primer1
     misc_feature    complement(240..263)
                     /label=Primer2
BASE COUNT      118 a    121 c     85 g    113 t      5 others
ORIGIN
        1 gatctctcct ttctcattag ggaagaaaat acgctgtgct aaatactata cttcattgac
       61 tattctcagg tcagaaagcg cactttcgac ttcttgtcct tccgtcgcgg agaggatgat
      121 ggcagctgcc aaaagtacat acttggaagt tcatcccagc acaaacacac acacacacac
      181 gccccccaca cacacacaca caaacacact cacacacaca cacgcacacg gtttcctagg
      241 taaagatttc ttccctgcca ttgntttacc taaaataagg caactgtgag gncactgtcc
      301 caacccggtt acactcctat tatatgtgcc tatcatcctg aggagtaatt tgattcaggt
      361 gttctggaag tcatgctgtg ggctgtgtct gttggattcc cagcgatgnc agggganaan
      421 cctgtgactc cttcctgaat tg
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human chromosome 4 sequence-tagged site STS4-329
Date: 21 Aug 92 10:09:00 GMT
Distribution: bionet
Checksum: 52336     3

LOCUS       HUM4STS329    484 bp ds-DNA             PRI       21-AUG-1992
DEFINITION  Human chromosome 4 sequence-tagged site STS4-329
ACCESSION   L00799
KEYWORDS    STS; human chromosome 4; sequence tagged site.
SOURCE      Homo sapiens, clone p4-273 from a chromosome 4 plasmid library;
            vector pBluescript II KS + (Stratagene)
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 484)
  AUTHORS   Goold,R.D., diSibio,G., Dugaiczyk,A., Smith,K.A., Xu,H.,
            Magrane,G., Lang,D.B., Cox,D.R., Masters,S.B. and Myers,R.M.
  TITLE     Sequence-Tagged Sites for Human Chromosome 4
  JOURNAL   Unpublished (1992) see COMMENT for author address
  STANDARD  full automatic
COMMENT     PCR components: 25 ng of human genomic DNA, 10 pmol of each
            oligonucleotide, 200 micro-M dNTPs, 0.25 U Taq  polymerase (Cetus)
            in 10 micro-l of 50 mM KCl-20 mM  Tris-HCl, pH 8.3 (at room temp),
            2.5 mM MgCl-2.  Initial denaturation at 94degC for 1.5 min, then 30
            cycles of  94degC for 15 sec, 64degC for 23 sec, and 72degC for 30
            sec, followed by a final extension at 72degC for 3.5 min, using a
            Perkin-Elmer 9600 thermocycler. PCR-amplified product size 220 bp.
            STS sequence contains a simple sequence tandem repeat (CA).
            Sequence submitted by:
            Human Genome Mapping Center
            Box 0925
            University of California San Francisco
            San Francisco, CA 94143-0925  USA
            Phone: (415) 502-1612   Fax: (415) 476-8391
            e-mail: hgmcprobes@cgl.ucsf.edu
FEATURES             Location/Qualifiers
     misc_feature    13..36
                     /label=Primer1
     misc_feature    complement(209..232)
                     /label=Primer2
BASE COUNT      151 a     74 c    102 g    148 t      9 others
ORIGIN
        1 gatcttaaat accacttgtc ccctaaagca tcttgtttgt ataaaataaa tcatacttta
       61 aaatgtncac aatttatgat attatatatt tttacatata atgtatattc acaactgcca
      121 gcatgtgaat atatatatta tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg
      181 tatntgcatg ctggcaggtg nagagattca gaattgcatc gaaggagaca ctttaaagga
      241 tgtgcatggn acaaaagaat tcattagatg ttccatttca aaattaaaat acacatttta
      301 catagtgaaa tttattatca acaattacca tatcaaaata tgaagagggt atcaagctaa
      361 gagtgctatg tacaaatgga tccactagtt cttagagcgg ccgccacncg gggggagctc
      421 caattcgccc tatagggagt cggattacaa aacactggnc gcggnntaca acgcngggat
      481 tggg
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human chromosome 4 sequence-tagged site STS4-54
Date: 21 Aug 92 10:10:59 GMT
Distribution: bionet
Checksum: 23286     3

LOCUS       HUM4STS54     400 bp ds-DNA             PRI       21-AUG-1992
DEFINITION  Human chromosome 4 sequence-tagged site STS4-54
ACCESSION   M95311
KEYWORDS    STS; human chromosome 4; sequence tagged site.
SOURCE      Homo sapiens, clone L4-37 from the C25 lambda library (Pritchard,
            Genomics 4, 408, 1989); vector - Lambda Dash II (Stratagene)
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 400)
  AUTHORS   Goold,R.D., diSibio,G., Dugaiczyk,A., Smith,K.A., Xu,H.,
            Magrane,G., Lang,D.B., Cox,D.R., Masters,S.B. and Myers,R.M.
  TITLE     Sequence-Tagged Sites for Human Chromosome 4
  JOURNAL   Unpublished (1992) see COMMENT for author address
  STANDARD  full automatic
COMMENT     PCR components: 25 ng of human genomic DNA, 40 pmol of each
            oligonucleotide, 200 micro-M dNTPs, 0.25 U Taq  polymerase (Cetus)
            in 10 micro-l of 50 mM KCl-20 mM  Tris-HCl, pH 8.3 (at room temp),
            2.5 mM MgCl-2.  Initial denaturation at 94degC for 1.5 min, then 30
            cycles of  94degC for 15 sec, 66degC for 23 sec, and 72degC for 30
            sec, followed by a final extension at 72degC for 3.5 min, using a
            Perkin-Elmer 9600 thermocycler. PCR-amplified product size 275 bp.
            Sequence submitted by:
            Human Genome Mapping Center
            Box 0925
            University of California San Francisco
            San Francisco, CA 94143-0925  USA
            Phone: (415) 502-1612   Fax: (415) 476-8391
            e-mail: hgmcprobes@cgl.ucsf.edu
FEATURES             Location/Qualifiers
     misc_feature    36..60
                     /label=Primer1
     misc_feature    complement(288..310)
                     /label=Primer2
BASE COUNT      113 a     80 c     85 g    103 t     19 others
ORIGIN
        1 ttattntntg ccttaaacgg atcatccgag tgggccctaa tccaatatga ttggtatccc
       61 tataaaaggg aaaaactaga catagagatg cacactgagc acaagataat aagaagacac
      121 agaacgtcat gtgaaggcac aggattgcag tggtatattt ccangcctaa atgcatttat
      181 accactaang attgttgttt antccaccac aaactaggta ggggcaagga aggattcctt
      241 tacaggtttt ggaggaagca tggtccngcc agcactctga ctttgaactt ctggcctcca
      301 gaactggaag aaaataaatt tctgttgntn ttncgccact cagtttgngg aantttgtta
      361 cngcagcccc ggnanattct cagngncgnt tnccnttgct
//