From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Toxoplasma gondii granule antigen (GRA2) gene, exons 1-2.
Date: 29 Dec 92 19:51:54 GMT
Distribution: bionet
Checksum: 16420 6

LOCUS       TOXGRA2X     1650 bp ds-DNA             INV       08-DEC-1992
DEFINITION  Toxoplasma gondii granule antigen (GRA2) gene, exons 1-2.
ACCESSION   L01753
KEYWORDS    granule antigen.
SOURCE      Toxoplasma gondii (strain RH) tachyzoite DNA.
  ORGANISM  Toxoplasma gondii
            Eukaryota; Animalia; Protozoa; Apicomplexa; Sporozoa; Coccidia;
            Eucoccidiidae; Eimeriina; Sarcocystidae.
REFERENCE   1  (bases 1 to 1650)
  AUTHORS   Prince,J.B., Araujo,F.G., Remington,J.S., Burg,J.L., Boothroyd,J.C.
            and Sharma,S.D.
  TITLE     Cloning of cDNAs encoding a 28 kilodalton antigen of Toxoplasma
            gondii
  JOURNAL   Mol. Biochem. Parasitol. 34, 3-13 (1989)
  STANDARD  full automatic
REFERENCE   2  (bases 1 to 1650)
  AUTHORS   PARMLEY,S.S., SGARLATO,G.G. and REMINGTON,J.J.
  TITLE     Genomic and corrected cDNA sequence of the P28 gene from Toxoplasma
            gondii
  JOURNAL   Mol. Biochem. Parasitol. (1992) In press
  STANDARD  full automatic
REFERENCE   3  (bases 1 to 1650)
  AUTHORS   Mercier,C., Lecordier,L., Darch,F., Deslee,D., Murray,A.,
            Tourvieille,B., Maes,P., Capron,A. and Cesbron Delauw,M.
  TITLE     Toxoplasma gondii : molecular characterization of a dense granule
            antigen (Gra 2) associated with the network of the parasitophorous
            vacuole
  JOURNAL   Mol. Biochem. Parasitol. (1993) In press
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     misc_feature    428..1618
                     /note="This feature applies to a gene which lacks a coding
                     region feature. "
                     /gene="GRA2"
     conflict        replace(1..335,"''")
                     /citation=[1]
     exon            326..576
                     /number=1
                     /evidence="EXPERIMENTAL"
                     /citation=[3]
     sig_peptide     428..496
                     /gene="GRA2"
                     /codon_start=1
                     /citation=[3]
                     /note="putative"
     intron          577..817
                     /number=1
                     /gene="GRA2"
                     /evidence="EXPERIMENTAL"
                     /citation=[3]
     conflict        replace(816,"ctgc")
                     /citation=[3]
                     /note="insertion of the additional 'CTGC' bases in
                     sequence [2]"
     exon            818..1618
                     /number=2
                     /evidence="EXPERIMENTAL"
                     /citation=[3]
     misc_feature    871..1098
                     /gene="GRA2"
                     /note="alpha-helical domain"
     conflict        replace(1274..1279,"gagaag")
                     /citation=[3]
                     /note="The 'GAGAAG' bases are not present in the sequence
                     [2]"
     polyA_site      1618
                     /gene="GRA2"
                     /evidence="EXPERIMENTAL"
                     /citation=[3]
BASE COUNT      431 a    382 c    455 g    382 t
ORIGIN      
        1 aaaaacaagt tcgtcgcaaa aggttaatta cctacgcacc acgaaggaaa acgcgtatca
       61 cgtcagtcct tacggtcaat atacaaatac ttgggccgtc cagtggaggc aacgtgcccg
      121 tcgcacggtg atactgactg gtgacttgca cgtacgtctc tcgccggcgt ccaaaccaaa
      181 ttgacccggg gcagcctact ccctgtcgtc ccttaggcta agtgcgagca acatctctac
      241 acagagacga cgccagagac gcaaaatgaa cagcggaacc tgcgtcgctg tctgtcctgc
      301 gaactgatga cagaaagggt cattaaacga tttcttttgc aattcgcgtc gttatcgcac
      361 gttgtttctc ttcccacgaa tagttgtttt gattagatat tgcttcttct ccacatatcg
      421 cctcacaatg ttcgccgtaa aacattgttt gctggttgtt gccgttggcg ccctggtcaa
      481 cgtctcggtg agggctgccg agttttccgg agttgttaac cagggaccag tcgacgtgcc
      541 tttcagcggt aaacctcttg atgagagagc agttgggtaa gttggcaaaa gtaatgatag
      601 aggcaggggt tgaacgatag gcggctgcag atttgtataa cacaacatga tgtagctgcc
      661 acggtttttt ttcggagagt gatgccgtct gactgttcat cgcacccatg ggagctaggg
      721 aggtgcgctt tctgtgtgat atgtattgtc ctagtccaat ttcccacgca ctgtagtgtc
      781 ttgagactcg gtgccatgta gaattttgtg tctgcagagg aaaaggtgaa catacaccac
      841 cactcccaga cgagaggcaa caagagccag aagaaccggt ttcccaacgt gcatccagag
      901 tggcagaaca actgtttcgc aagttcttga agttcgctga aaacgtcgga catcacagtg
      961 agaaggcctt caaaaaagca aaggtggtgg cagaaaaagg cttcaccgcg gcaaaaacgc
     1021 acacggttag gggtttcaag gtggccaaag aagcagctgg aaggggcatg gtgaccgttg
     1081 gcaagaaact cgcgaatgtg gagagtgaca gaagcactac gacaacgcag gcccccgaca
     1141 gccctaatgg cctggcagaa accgaggttc cagtggagcc ccaacagcgg gccgcacacg
     1201 tgcccgtccc agacttttcg cagtaatgtt gactacgacg aaagtgatgc gcaggctgga
     1261 aagccgctga agggagaagt ctacaaagcc gatcagtgaa aaatgtgtgg ggaggtggtc
     1321 ttgttgcagg aatgcaatgg tgttaagcat cgtgttcgaa tgcagtgcgt gtatcagttg
     1381 tgcgcggaag gacactgctt caatgttaag aacctgtttt ctccgtagag aggaccaaaa
     1441 gacgattgca aaactggtat gtacgcaata gcccaatgcc ggacgtcagt tggttgtatg
     1501 tgacgctccc agatgtcata tgccttgtga gtgtgtctgg gatgcaagtt tttggtgtgc
     1561 gttgatttcg ccagcttatg acagtggcag acgaattatt gacatgatac aaggacgcag
     1621 aaaggaacaa acaccgtagt tccagtcgac
//