From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Plasmid pGT633 (from Lactobacillus reuteri) methylase (ermGT) gene,
Date: 27 Jan 93 05:47:28 GMT
Distribution: bionet
Checksum: 31530 5

LOCUS       G63ERMGT      938 bp ds-DNA             BCT       26-JAN-1993
DEFINITION  Plasmid pGT633 (from Lactobacillus reuteri) methylase (ermGT) gene,
            complete cds and leader peptide.
ACCESSION   M64090
KEYWORDS    MLS antibiotics resistance; erythromycin resistance; methylase.
SOURCE      Plasmid pGT633 DNA.
  ORGANISM  Plasmid pGT633
            Prokaryotae.
REFERENCE   1  (bases 1 to 938)
  AUTHORS   Tannock,G.W., Luchansky,J.B., Miller,L.A., Connell,H., Mercer,A.A.
            and Klaenhammer,T.R.
  TITLE     Characterization of a plasmid (pGT633) from Lactobacillus reuteri
            that encodes resistance to MLS antibiotics
  JOURNAL   Unpublished (1993)
  STANDARD  full automatic
COMMENT     *natural host strain = 100-63.
FEATURES             Location/Qualifiers
     stem_loop       111..149
                     /function="2:3 stem loop prediction for `on' conformation"
                     /note="hairpin that leaves open the ribosomal binding site
                     for ermGT methylase"
     stem_loop       138..172
                     /function="3:4 hairpin prediction for `off' conformation"
                     /note="secondary stem loop prediction that covers the
                     ribosomal binding site of the ermR methylase; free energy
                     at -16.4 kcal"
     stem_loop       898..938
                     /function="terminator"
                     /note="secondary stem loop prediction that covers the
                     termination codon of ermGT methylase; free energy
                     prediction at -21.1 kcal"
     CDS             23..82
                     /function="inoperative translational attenuator"
                     /gene="ermGT"
                     /evidence="EXPERIMENTAL"
                     /note="94.7% homologous to ermC leader peptide; ermGT
                     methylase is constitutively expressed indicating that the
                     ermGT leader does not function as a translational
                     attenuator"
                     /product="leader peptide"
                     /codon_start=1
                     /translation="MGIFSIFVINTVHYQPNKK"
     CDS             168..902
                     /function="erythromycin resistance"
                     /gene="ermGT"
                     /evidence="EXPERIMENTAL"
                     /note="77.5% identity with ermC methylase; methylase
                     confers resistance to erythromycin in various gram
                     postitive and gram negative bacterial hosts"
                     /product="methylase"
                     /codon_start=1
                     /translation="MNKKNIKDSQNFITSKHHINEILRNVHLNTNDNIIEIGSGKGHF
                     SFELAKRCNYVTAIEIDPKLCRITKNKLIEYENFQVINKDILQFKFPKNKSYKIFGNI
                     PYNISTDIIRKIVFESTATESYLIVEYGFAKRLLNTNRSLALFLMTEVDISILSKIPR
                     EYFHPKPRVNSSLIVLKRHPSKISLKDRKQYENFVMKWVNKEYIKLFSKNQFYQALKY
                     ARIDDLNNISFEQFLSLFNSYKLFNR"
BASE COUNT      395 a    119 c    125 g    299 t
ORIGIN      
        1 cataaaaaag gagaattaat atatgggcat ttttagtatt tttgtaatca acacagttca
       61 ttatcaacca aacaaaaaat aagtggttat caaccaaaca aaaaataagt ggttataatg
      121 gattgttaat ataaacattc attataacct cataggagtg ggttataatg aacaaaaaaa
      181 atataaaaga tagtcaaaac tttattactt caaagcatca tataaatgaa attttgagaa
      241 atgtacattt aaatacaaat gataatatta ttgagattgg ttcagggaaa ggtcatttct
      301 cgtttgaatt agctaaaagg tgtaattatg taaccgccat tgaaatagat cctaaattat
      361 gtaggataac taaaaacaaa cttattgaat atgagaactt ccaggttatc aataaagata
      421 ttttacaatt taagtttcct aaaaataagt catataagat atttggaaat ataccctaca
      481 atataagtac agatataatt cgaaaaattg tttttgaaag cacagctaca gaaagttatt
      541 taatagtgga atatggattt gctaaaaggt tgctaaatac aaatcgttca ctagcactat
      601 ttttaatgac agaagttgat atatccatat taagtaaaat ccctagagaa tactttcatc
      661 caaaacctag agttaatagc tcgttaattg tattaaaaag acacccttca aaaatatcac
      721 tcaaagatag aaaacaatat gaaaattttg ttatgaaatg ggttaacaaa gaatacataa
      781 aactattttc caaaaaccaa ttttatcaag ccttaaaata tgcaagaatt gacgatttaa
      841 acaatattag ctttgaacaa ttcttgtctc ttttcaatag ctataaatta tttaatagat
      901 aagttaaggg gtgcataaac tgcatccctt aacttatc
//