From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Streptomyces mycarofaciens acyltransferase (mdmB) and
Date: 15 Sep 92 10:06:09 GMT
Distribution: bionet
Checksum: 52637     6

LOCUS       STMMDMBC     2381 bp ds-DNA             BCT       15-SEP-1992
DEFINITION  Streptomyces mycarofaciens acyltransferase (mdmB) and
            O-methyltransferase (mdmC) genes, complete cds.
ACCESSION   M93958
KEYWORDS    O-methyltransferase; macrolide 3-O-acetyltransferase.
SOURCE      Streptomyces mycarofaciens (library: ATCC 21454) DNA.
  ORGANISM  Streptomyces mycarofaciens
            Prokaryotae; Firmicutes; Streptomycetaceae.
REFERENCE   1  (bases 1 to 2381)
  AUTHORS   Hara,O. and Hutchinson,C.R.
  TITLE     A macrolide 3-O-acyltransferase gene from the midecamycin-producing
            species Streptomyces mycarofaciens
  JOURNAL   J. Bacteriol. 174, 5141-5144 (1992)
  STANDARD  simple staff_entry
FEATURES             Location/Qualifiers
     CDS             126..1289
                     /function="catalyzes the addition of acyl groups to the 3
                     position of 16-membered macrolide antibiotics"
                     /product="acyltransferase"
                     /gene="mdmB"
                     /codon_start=1
                     /translation="MPPRVVRLPSLTGLRWFAALAVFACHIAQQQFFADQQVGTALLH
                     ITTLGSIAVSVFFLLSGFVLAWSARDKDSVTTFWRRRFAKIYPLHLVTFLIAGVIIFS
                     LAEPTLPGGSVWDGLVPDLLLVQSWLPEPTIIAGFNTPSWSLSCEFAFYLTFPLWYRL
                     VRKIPVRRLWWCAAGIAAAVICVPFVTSQFPASAETAPGMPLNELWFACWLPPVRMLE
                     FVLGIVMALILRTGVWRGPGVVSSALLLAAAYGVTQVVPPMFTIAACSIVPAALLITA
                     LANADVQGLRTGLRSAVLVRLGEWSFAFYLVHFMVIRYGHRLMGGELGYARQWSTASA
                     GALALAMLAVAIVAGGLLHTVVENPCMRLLGRRRPVATAPDPATDEAPKLTRA"
     CDS             1327..1992
                     /function="biosynthesis of 16-membered macrolide
                     antibiotics"
                     /product="O-methyltransferase"
                     /gene="mdmC"
                     /note="putative"
                     /codon_start=1
                     /translation="MADQTTLSPALLDYARSVALREDGLLRELHDMTAQLPGGRAMQI
                     MPEEAQFLGLLIRLVGARRVLEIGTFTGYSTLCMARALPAGGRIVTCDISDKWPGIGA
                     PFWQRAGVDGLIDLRIGDAARTLAELRERDGDGAFDLVFVDADKAGYLHYYEQALALV
                     RPGGLVAIDNTLFFGRVADPAADDPDTVAVRTLNDLLRDDERVDIALLTVADGITLAR
                     RRE"
BASE COUNT      341 a    813 c    783 g    444 t
ORIGIN
        1 gtcgaccgtt gactgtgtca tctccgcgtt gtcggccgaa ttgctcgcgc cgcagactgc
       61 cctgaccgtt tctctgcgtt cacccggcct cacttttccc cctccagaag gagagcgagc
      121 aatcgatgcc gcctcgtgtc gtccgcctgc cgtcgctcac cggccttcgc tggttcgcgg
      181 ccttagcggt attcgcctgt cacattgccc agcagcaatt cttcgctgac cagcaggtcg
      241 gaaccgcgct gctacacatc accacgctcg gttcgatcgc ggtctcggtc ttcttcctgc
      301 tcagcggttt tgttctggcg tggtcggccc gtgacaagga ctctgtcacg actttctggc
      361 ggcgccgatt tgccaagatc tacccgctgc acctcgtaac tttcctcata gcgggcgtca
      421 tcattttctc cctcgcggag ccgactctgc ccggtggttc cgtatgggac ggtctggttc
      481 ccgatcttct gctggtgcag tcctggcttc ccgaacccac gattatcgcc gggttcaaca
      541 ccccgagttg gtcgctctcc tgtgaattcg ctttctatct gacgttcccg ctgtggtatc
      601 ggctggtgcg gaagattccg gtacggcggc tgtggtggtg tgccgccggt attgccgcgg
      661 ccgtgatttg tgtaccgttc gtgacgagcc agttcccggc gagcgccgag acggcccccg
      721 ggatgccgct caacgagctg tggttcgcct gctggctgcc gccggtgcgg atgctggagt
      781 tcgtcctcgg catcgtgatg gcgctgatcc tgcgcacggg cgtgtggcgg ggccccggag
      841 tggtgtcctc cgcgctgctg ctcgccgcgg cctacggcgt cacccaggtg gtgcccccga
      901 tgttcaccat cgccgcgtgc tccatcgtgc ccgccgccct gctgatcacc gctttggcca
      961 atgccgatgt gcagggcctg cgcaccggtt tgcgctcggc ggtactggtg cggctgggcg
     1021 agtggtcctt cgccttctat ctggttcact tcatggtcat ccgctacggg caccggctga
     1081 tgggcggcga actgggctac gcgcgccagt ggagcaccgc gagcgcggga gcgctggccc
     1141 tggcgatgct ggcggtcgcg atcgtggccg gtgggctgct gcacaccgtc gtggagaacc
     1201 cctgcatgcg tctgctcggc cgccgccgcc cggtcgccac cgcaccagac cccgcaaccg
     1261 acgaggctcc gaaactcacc cgcgcctgac gtcaccgaac aagctttcct ggaaaggact
     1321 gtgagcgtgg cagaccagac cactctcagc cccgcgctgc tggactacgc ccgaagcgtc
     1381 gctctgcggg aagacggcct gctgcgggag ctgcacgaca tgaccgcgca gctccccggg
     1441 gggcgtgcca tgcagatcat gcccgaggag gcgcagttcc tcggtctgct gatccggctc
     1501 gtcggcgccc ggcgggtgct ggagatcggg acgttcaccg gttacagcac gctgtgcatg
     1561 gcacgggcac tgcctgccgg cggccggatc gtcacctgcg acatcagcga caagtggccc
     1621 gggattggcg ccccgttctg gcaacgcgcc ggggtggacg gcctgatcga cctgcgcatc
     1681 ggcgacgccg cccggacact cgccgagctg cgggagcgcg acggggacgg cgcgttcgac
     1741 ctggtcttcg tcgacgccga caaggccggg tacttgcact actacgagca ggcgctggca
     1801 ctggtccgcc ccggcgggct ggtggcgatc gacaacaccc tgttcttcgg ccgagtggcc
     1861 gacccggccg ccgacgaccc cgacacagtg gccgtgcgca ccctcaacga cctgctgcgc
     1921 gacgacgagc gcgtggacat cgctctgctg accgtcgccg atgggatcac cctcgcccga
     1981 cgacgggagt aagtaccgcg acccggtgcc gcggccgacg actcggacgc ctcgctgaag
     2041 agggacgaca gttgctgtcg acaggatggt tccgggccgg tgctcagggc ccgtccccac
     2101 tctggtatgc gccgccggta tcggcggggt gtgaagcccg gaggagaaac ccaagggctc
     2161 cgcggaggag aaggccggcg tctccgctgg cgttccggac aaccggccgg atacgggctt
     2221 cttgcccggc cccgaaaggg tgcttacgtg ggtcaggtgg cctgcgagga accgatgaac
     2281 atcaaatgcc ggaatccaag gacaagttgg acaccatgac gaccgcaacc accacggcat
     2341 gctgctgatc aagccgagta gagcggccgt gcgacggatc c
//