From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: mxiM=lipoprotein...mxiK [Shigella flexneri, virulence plasmid
Date: 27 Jan 93 05:49:25 GMT
Distribution: bionet
Checksum: 51799 6

LOCUS       M259598S1    1701 bp    DNA             BCT       19-JAN-1993
DEFINITION  mxiM=lipoprotein...mxiK [Shigella flexneri, virulence plasmid
            pWR100, Plasmid, 6 genes, 1701 nt, segment 1 of 2] 
ACCESSION   S49866 M98390
KEYWORDS    .
SEGMENT     1 of 2
SOURCE      Shigella flexneri virulence plasmid pWR100
  ORGANISM  Shigella flexneri
            Unclassified.
REFERENCE   1  (bases 1 to 1701)
  AUTHORS   Allaoui,A., Sansonetti,P.J. and Parsot,C.
  TITLE     MxiJ, a lipoprotein involved in secretion of Shigella Ipa invasins,
            is homologous to YscJ, a secretion factor of the Yersinia Yop
            proteins.
  JOURNAL   J. Bacteriol. 174, 7661-7669 (1992)
  STANDARD  full automatic
COMMENT     This entry [NCBI gibbsq 119112] was created by the journal scanning
            component of NCBI/GenBank at the National Library of Medicine.
            This sequence comes from Fig. 2.
FEATURES             Location/Qualifiers
     CDS             188..439
                     /note="YscF homolog;  For the protein sequence (NCBI
                     gibbsq 119115):  Method: conceptual translation supplied
                     by author.  This sequence comes from Fig. 2."
                     /gene="mxiH"
                     /product="MxiH"
                     /codon_start=1
                     /translation="MSVTVPNDDWTLSSLSETFDDGTQTLQGELTLALDKLAKNPSNP
                     QLLAEYQSKLSEYTLYRNAQSNTVKVIKDVDAAIIQNFR"
     CDS             452..745
                     /note="For the protein sequence (NCBI gibbsq 119116):
                     Method: conceptual translation supplied by author.  This
                     sequence comes from Fig. 2."
                     /gene="mxiI"
                     /product="MxiI"
                     /codon_start=1
                     /translation="MNYIYPVNQVDIIKASDFQSQEISSLEDVVSAKYSDIKMDTDIQ
                     VSQIMEMVSNPESLNPESLAKLQTTLSNYSIGVSLAGTLARKTVSAVETLLKS"
     CDS             751..1476
                     /note="Ipa invasin secretion factor;  For the protein
                     sequence (NCBI gibbsq 119117):  Method: conceptual
                     translation supplied by author.  This sequence comes from
                     Fig. 2."
                     /gene="mxiJ"
                     /product="MxiJ"
                     /codon_start=1
                     /translation="MIRYKGFILFLLLMLIGCEQREELISNLSQRQANEIISVLERHN
                     ITARKVDGGKQGISVQVEKGTFASAVDLMRMYDLPNPERVDISQMFPTDSLVSSPRAE
                     KARLYSAIEQRLEQSLVSIGGVISAKIHVSYDLEEKNISSKPMHISVIAIYDSPKESE
                     LLVSNIKRFLKNTFSDVKYENISVILTPKEEYVYTNVQPVKEVKSEFLTNEVIYLFLG
                     MAVLVVILLVWAFKTGWFKRNKI"
     CDS             1473..1700
                     /partial
                     /note="For the protein sequence (NCBI gibbsq 119118):
                     Method: conceptual translation supplied by author.  This
                     sequence comes from Fig. 2."
                     /gene="mxiK"
                     /product="MxiK"
                     /codon_start=1
                     /translation="MIRMDGIYKKYLSIIFDPAFYINRNRLNLPSELLENGVIRSEIN
                     NLIINKYDLNCDIEPLSGVTAMFVANWNLLPA"
BASE COUNT      596 a    235 c    327 g    543 t
ORIGIN      
        1 aagcttgagc tcattaatag actgatttct gaacataaaa atatatatgg agatcagtat
       61 attgagtttt ctgtactttt gatagatgat gattttaaag gtaaatcata tcttaacagc
      121 aaagacagtt atgtaatgtt gaatgataaa cactggtttt ttttagataa aaataagtga
      181 ggataaaatg agtgttacag taccgaatga tgattggaca ttgagttcat tatctgaaac
      241 ttttgatgat ggaactcaaa cattacaagg tgaactaaca ttggcactag ataaattagc
      301 taaaaatcct tcgaatccac agttgctggc tgaataccaa agtaaattat ctgaatatac
      361 attatatagg aacgcgcaat ccaatacagt gaaagtgatt aaggatgttg atgctgcaat
      421 tattcaaaac ttcagataat agggagcatt catgaattac atttatccag tcaatcaggt
      481 tgatattatc aaagccagtg attttcaatc tcaagagata tcaagtctgg aagacgtcgt
      541 gtcggctaaa tatagtgata ttaagatgga tacagatatt caagtatcac aaataatgga
      601 gatggtaagc aatccagaat cattaaaccc agaatctttg gccaagttac agacgacgct
      661 ctcaaattat tcaataggag tatcattagc tggcacgtta gcaagaaaaa cagtttcggc
      721 tgttgaaact ttattaaagt cttaatttat atgattaggt ataaaggttt tattttattc
      781 ttgttgctga tgttgattgg atgtgagcaa cgtgaagagt taatttctaa tttatctcaa
      841 agacaggcaa atgaaataat atctgtgcta gaacgccata atattactgc tagaaaagtt
      901 gatggaggta aacaggggat ctcggtacaa gtcgaaaagg ggacatttgc atcggcagtt
      961 gatttgatgc gcatgtacga tttgccaaat ccggagagag ttgatatctc acaaatgttt
     1021 cctacagatt cattagtgtc ttctccaaga gctgaaaagg cccgtttata tagtgctatt
     1081 gagcaacggc tggaacagtc tttagtttct attggtggtg ttatttcggc aaaaatacat
     1141 gttagctatg atcttgaaga aaaaaatata tcttcaaaac cgatgcatat atcagtaatc
     1201 gctatatatg actcaccgaa agagtctgaa ctattagtta gtaatattaa gcgatttttg
     1261 aaaaacacct tttctgatgt taagtatgaa aatatatctg tcatattaac tccgaaagaa
     1321 gaatatgttt atacaaatgt acaacctgtt aaggaagtta aatcggaatt tttaacaaat
     1381 gaagtaatat atttatttct cgggatggct gtactagttg tcattctttt ggtatgggca
     1441 ttcaaaacag ggtggttcaa gagaaacaaa atatgataag aatggatgga atttataaaa
     1501 aatatctttc aataattttt gatccagcgt tctatataaa tagaaatcgg ttgaatttgc
     1561 cttctgaact gttagaaaat ggcgtaatca gaagtgagat taataatctc ataattaata
     1621 aatatgatct aaattgcgat attgaacctt taagcggggt aaccgctatg tttgttgcca
     1681 actggaattt acttccagct g
//