From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Peptostreptococcus magnus protein L gene, complete cds.
Date: 28 Dec 92 21:30:54 GMT
Distribution: bionet
Checksum: 60101 9

LOCUS       PEPPROTEIN   3258 bp ds-DNA             BCT       01-DEC-1992
DEFINITION  Peptostreptococcus magnus protein L gene, complete cds.
ACCESSION   L04466
KEYWORDS    protein L.
SOURCE      Peptostreptococcus magnus (strain 3316) DNA.
  ORGANISM  Peptostreptococcus magnus
            Prokaryotae; Firmicutes; Firmibacteria; Gram-positive cocci;
            Peptococcaceae.
REFERENCE   1  (bases 1 to 3258)
  AUTHORS   Murphy,J.P., Trowern,A.R. and Duggleby,C.J.
  TITLE     Nucleotide sequence of peptostreptococcus protein L
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     sig_peptide     208..279
                     /codon_start=1
     mat_peptide     280..3183
                     /product="protein L"
                     /codon_start=1
     repeat_unit     490..573
                     /function="unknown"
                     /rpt_type=direct
                     /label=A-repeats
                     /note="3 A-REPEATS A1 (490-573); A2 (673-756); A3
                     (856-948)"
     repeat_unit     574..672
                     /function="UNKNOWN"
                     /rpt_type=direct
                     /label=B-REPEATS
                     /note="2 B-REPEATS B1 (574-672) B2 (757-855)"
     protein_bind    949..1596
                     /function="POSSIBLE VIRULENCE MECHANISM"
                     /bound_moiety="IMMUNOGLOBULIN KAPPA LIGHT CHAIN"
                     /evidence="EXPERIMENTAL"
                     /label=C-REPEATS
     repeat_unit     949..1161
                     /function="BINDS IMMUNOGLOBULIN KAPPA LIGHT CHAINS"
                     /rpt_type=direct
                     /evidence="EXPERIMENTAL"
                     /label=C-REPEATS
                     /note="4 C-REPEATS C1 (949-1161) C2 (1162-1374) C3
                     (1375-1596) C4(1597-1.."
     repeat_unit     1822..2034
                     /function="BINDS TO SERUM ALBUMIN"
                     /rpt_type=direct
                     /evidence="EXPERIMENTAL"
                     /label=D-REPEATS
                     /note="4 D-REPEATS D1 (1822-2034) D2 (2347-2544) D3
                     (2545-2730) D4(2731-2913)"
     repeat_unit     2035..2208
                     /function="UNKNOWN"
                     /rpt_type=direct
                     /label=E-REPEATS
                     /note="2 E-REPEATS E1 (2035-2208) E2 (2209-2346);
                     putative"
     repeat_region   2914..3051
                     /function="CHARGED CELL ANCHOR"
                     /rpt_type=tandem
                     /rpt_unit=2914..2934
                     /label=R-REPEATS
                     /note="8 R-REPEATS; putative"
     protein_bind    3052..3183
                     /function="ATTACH PROTEIN TO CELL SURFACE"
                     /bound_moiety="CELL ANCHOR REGION"
                     /label=M-REGION
                     /note="putative"
     CDS             208..3186
                     /product="protein L"
                     /codon_start=1
                     /translation="MKINKKLLMAALAGAIVVGGGANAYAAEEDNTDNNLSMDEISDA
                     YFDYHGDVSDSVDPVEEEIDEALAKALAEAKETAKKHIDSLNHLSETAKKLAKNDIDS
                     ATTINAINDIVARADVMERKTAEKEEAEKLAAAKETAKKHIDELKHLADKTKELAKRD
                     IDSATTINAINDIVARADVMERKTAEKEEAEKLAAAKETAKKHIDELKHLADKTKELA
                     KRDIDSATTIDAINDIVARADVMERKLSEKETPEPEEEVTIKANLIFADGSTQNAEFK
                     GTFAKAVSDAYAYADALKKDNGEYTVDVADKGLTLNIKFAGKKEKPEEPKEEVTIKVN
                     LIFADGKTQTAEFKGTFEEATAKAYAYADLLAKENGEYTADLEDGGNTINIKFAGKET
                     PETPEEPKEEVTIKVNLIFADGKIQTAEFKGTFEEATAKAYAYANLLAKENGEYTADL
                     EDGGNTINIKFAGKETPETPEEPKEEVTIKVNLIFADGKTQTAEFKGTFEEATAEAYR
                     YADLLAKVNGEYTADLEDGGYTINIKFAGKEQPGENPGITIDEWLLKNAKEEAIKELK
                     EAGITSDLYFSLINKAKTVEGVEALKNEILKAHAGEETPELKDGYATYEEAEAAAKEA
                     LKNDDVNNAYEIVQGADGRYYYVLKIEVADEEEPGEDTPEVQEGYATYEEAEAAAKEA
                     LKEDKVNNAYEVVQGADGRYYYVLKIEDKEDEQPGEEPGENPGITIDEWLLKNAKEDA
                     IKELKEAGISSDIYFDAINKAKTVEGVEALKNEILKAHAEKPGENPGITIDEWLLKNA
                     KEAAIKELKEAGITAEYLFNLINKAKTVEGVESLKNEILKAHAEKPGENPGITIDEWL
                     LKNAKEDAIKELKEAGITSDIYFDAINKAKTIEGVEALKNEILKAHKKDEEPGKKPGE
                     DKKPEDKKPGEDKKPEDKKPGEDKKPEDKKPGKTDKDSPNKKKKAKLPKAGSEAEILT
                     LAAAALSTAAGAYVSLKKRK"
BASE COUNT     1495 a    477 c    623 g    663 t
ORIGIN      
        1 tttggacagt ggacgaaaca agaacactga tttaataaat tggtgaaatt cgattgttga
       61 aatacctttt tgggtagaaa taactaagga atggcaatat aattgcttgg aaacgaattt
      121 gatttaaata gcattaaatg caaaaaattt aaaaggagga gacaaattcc accctttata
      181 aagggaagtt tccattgtca aaataatatg aagattaata agaaattatt aatggctgca
      241 cttgcaggag caattgtagt tggtggtgga gctaacgctt acgcagctga agaagataac
      301 actgataata acctttcaat ggatgaaatt agtgatgctt attttgatta tcacggagat
      361 gtttcagatt cagtagatcc tgtagaagaa gaaatagacg aagcattagc aaaagcatta
      421 gcagaagcta aagaaacagc aaaaaaacat atagattctt taaatcattt gtcagaaaca
      481 gcaaaaaaat tagctaagaa tgatatagat tcagctacta ctattaatgc aatcaatgac
      541 atcgtagcaa gagcagatgt aatggaaaga aaaacagctg aaaaagaaga agcagaaaaa
      601 ttagcagcag caaaagaaac agcaaagaaa catatagatg aattaaaaca cttagcagac
      661 aaaacaaaag aattagctaa gagagatata gattcagcta ctactattaa tgcaatcaat
      721 gacatcgtag caagagcaga tgtaatggaa agaaaaacag ctgaaaaaga agaagcagaa
      781 aaattagcag cagcaaaaga aacagcaaag aaacatatag atgaattaaa acacttagca
      841 gacaaaacaa aagaattagc taagagagat atagattcag ctactactat tgatgcaatc
      901 aatgatatcg tagctagagc agatgtaatg gaaagaaagt tatctgaaaa agaaacacca
      961 gaaccagaag aagaagttac aatcaaagct aacttaatct ttgcagatgg aagcacacaa
     1021 aatgcagaat tcaaaggaac attcgcaaaa gcagtatcag atgcttacgc ttacgcagat
     1081 gctttaaaga aagacaacgg agaatatact gtagacgttg cagataaagg cttaacttta
     1141 aatattaaat tcgctggtaa aaaagaaaaa ccagaagaac caaaagaaga agttacaatc
     1201 aaagttaact taatctttgc agatggaaag acacaaacag cagaattcaa aggaacattt
     1261 gaagaagcaa cagcaaaagc ttatgcttat gcagacttat tagcaaaaga aaatggcgaa
     1321 tatacagcag acttagaaga tggtggaaac acaatcaaca ttaaatttgc tggaaaagaa
     1381 acaccagaaa caccagaaga accaaaagaa gaagttacaa tcaaagttaa cttaatcttt
     1441 gcagatggaa agatacaaac agcagaattc aaaggaacat ttgaagaagc aacagcaaaa
     1501 gcttatgctt atgcaaactt attagcaaaa gaaaatggcg aatatacagc agacttagaa
     1561 gatggtggaa acacaatcaa cattaaattt gctggaaaag aaacaccaga aacaccagaa
     1621 gaaccaaaag aagaagttac aatcaaagtt aacttaatct ttgcagatgg aaaaacacaa
     1681 acagcagaat tcaaaggaac atttgaagaa gcaacagcag aagcttacag atatgcagac
     1741 ttattagcaa aagtaaatgg tgaatacaca gcagacttag aagatggcgg atacactatc
     1801 aacatcaaat ttgctggaaa agaacaacca ggcgaaaatc caggaatcac aattgatgaa
     1861 tggttattaa agaatgctaa agaagaagca atcaaagaat taaaagaagc aggaatcact
     1921 tctgatttat acttcagctt aatcaataaa gcaaaaacag ttgaaggcgt agaagcatta
     1981 aagaacgaaa tcttaaaagc acacgctgga gaagaaacac cagaattaaa agatggatat
     2041 gcaacatatg aagaagcaga agcagcagct aaagaagctt tgaaaaatga tgatgttaac
     2101 aacgcatacg aaatagttca aggtgcagac ggaagatact actatgtatt aaagattgaa
     2161 gttgcagacg aagaagaacc aggtgaagac actccagaag ttcaagaagg ttacgcaact
     2221 tacgaagaag cagaagcagc agctaaagaa gcattaaaag aagataaagt taacaatgca
     2281 tacgaagtag ttcaaggtgc agacggaaga tactactatg tattaaaaat cgaagataaa
     2341 gaagatgaac aaccaggtga agaaccaggc gaaaacccag gaatcacaat tgatgaatgg
     2401 ttattaaaga atgctaaaga agacgcaatc aaagaattaa aagaagcagg aatcagttct
     2461 gacatatact ttgatgcaat caacaaagca aaaacagtag aaggcgtaga agcgttaaag
     2521 aacgaaatct taaaagcaca cgctgaaaaa ccaggcgaaa acccaggaat cacaattgat
     2581 gaatggttat taaagaatgc taaagaagct gcaatcaaag aattaaaaga agcaggaatc
     2641 actgctgaat atctattcaa cttaatcaac aaagcaaaaa cagtagaagg cgtagaatca
     2701 ttaaagaacg aaatcttaaa agcacacgct gaaaaaccag gcgaaaaccc aggaatcaca
     2761 attgatgaat ggttattaaa gaacgctaaa gaagatgcaa ttaaagaatt aaaagaagca
     2821 ggaattactt ctgacatata ctttgatgct atcaacaaag caaaaactat tgaaggcgta
     2881 gaagcattaa agaatgaaat cttaaaggct cataaaaaag atgaagaacc aggtaaaaaa
     2941 ccaggtgaag acaaaaaacc agaagataaa aaaccaggtg aagataaaaa accagaagac
     3001 aaaaaacctg gtgaagataa aaaaccagaa gacaaaaaac caggtaaaac agataaagat
     3061 tctccaaata agaagaaaaa agctaaatta ccaaaagctg gtagcgaagc tgaaatctta
     3121 acattagcag cagcagcttt atcaacagca gcaggtgctt acgtttcact taaaaaacgt
     3181 aaataattaa tcttagataa agaatagatt aatataaaaa atgggactta taatagtccc
     3241 attttttaat gcgaaaaa
//