From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Rat glutathione S-transferase Yb subunit gene, complete cds.
Date: 2 Sep 92 10:01:27 GMT
Distribution: bionet
Checksum: 54989    10

LOCUS       RATGSTYD     5224 bp ds-DNA             ROD       02-SEP-1992
DEFINITION  Rat glutathione S-transferase Yb subunit gene, complete cds.
ACCESSION   J03914
KEYWORDS    glutathione S-transferase.
SOURCE      Rat (Sprague-Dawley, adult) liver DNA, clone lambda-GTR4-1.
  ORGANISM  Rattus norvegicus
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Rodentia; Myomorpha; Muridae; Murinae.
REFERENCE   1  (bases 1 to 5224)
  AUTHORS   Lai,H.-C.J., Qian,B., Grove,G. and Tu,C.-P.D.
  TITLE     Gene expression of rat glutathione S-transferase: Evidence for gene
            conversion in the evolution of the Yb multigene family
  JOURNAL   J. Biol. Chem. 263, 11389-11395 (1988)
  STANDARD  simple staff_entry
COMMENT     Exon 8 ends just beyond the poly-A addition site
            Draft entry and computer-readable sequence for [1] kindly provided
            by H.-C.J.Lai, 08-JUN-1988.
FEATURES             Location/Qualifiers
     exon            <405..440
                     /number=1
                     /note="glutathione S-transferase Yb subunit,  (EC
                     2.5.1.18)"
     CDS             join(405..440,709..784,1104..1168,1455..1536,1634..1734,
                     2600..2695,2777..2887,4672..4761)
                     /note="glutathione S-transferase Yb subunit"
                     /codon_start=1
                     /translation="MPMTLGYWDIRGLAHAIRLFLEYTDTSYEDKKYSMGDAPDYDRS
                     QWLSEKFKLGLDFPNLPYLIDGSHKITQSNAILRYLGRKHNLCGETEEERIRVDVLEN
                     QAMDTRLQLAMVCYSPDFERKKPEYLEGLPEKMKLYSEFLGKQPWFAGNKITYVDFLV
                     YDVLDQHRIFEPKCLDAFPNLKDFVARFEGLKKISDYMKSGRFLSKPIFAKMAFWNPK
                     "
     prim_transcript 372..>5096
                     /note="GST mRNA and introns"
     intron          441..708
                     /note="GST intron A"
     exon            709..784
                     /number=2
     intron          785..1103
                     /note="GST intron B"
     exon            1104..1168
                     /number=3
     intron          1169..1454
                     /note="GST intron C"
     exon            1455..1536
                     /number=4
     intron          1537..1633
                     /note="GST intron D"
     exon            1634..1734
                     /number=5
     intron          1735..2599
                     /note="GST intron E"
     exon            2600..2695
                     /number=6
     intron          2696..2776
                     /note="GST intron F"
     exon            2777..2887
                     /number=7
     intron          2888..4671
                     /note="GST intron G"
     exon            4672..>4761
                     /number=8
                     /note="glutathione S-transferase Yb subunit"
BASE COUNT     1125 a   1345 c   1345 g   1409 t
ORIGIN      708 bp upstream of PvuII site.
        1 aaggcaggga gttgacggtg aagaaggaac aatgccagga agataatgaa cagggttgca
       61 ctttgcccat caaaagtctc taactgcaca tctggtggaa acagcacacc agagcaagaa
      121 cagggagctg ccgacacagg tgctagccca ttcctgtctt tgattactga ctgcttagac
      181 tggttcctga gccagtatag atatattttt ttccctgtcc ctggatccat agctccttcc
      241 cccaacaatc cctcccgtgg aatgtctgaa atttgaaaca ctgtaggcca atggtccaat
      301 agaaagccat aaccaggtcc cgcctcctcc ttgcctaaga cattatcagg aagctcagac
      361 ttgcaagacc caggtttgtc tgctctgtac accctaccag cacgatgcct atgacactgg
      421 gttactggga catccgtggg gtgagtgaga gcctcttctg ctgggtggga catgtgtggg
      481 gtgaggagta gctaggatgt gatttccagg agacagattg agtgctgaag ttgttggaaa
      541 gttttgcctc tcagggcaag ttcaaacaca ggggggcttg tgcttatgtg gcctgtgtgt
      601 gagcttgtgt cgtgactgtt cagtgtaagg tgtaggctca ccaagagatt gcacagtcag
      661 actgtcaggc ttttccaatc tgtgacttgt gatatgtcat cttcccagct ggctcacgcc
      721 attcgcctgt tcctggagta tacagacaca agctatgagg acaagaagta cagcatgggg
      781 gatggtgatt acacctgctt ctcagacccc tgctcccctg accctggtgc tcagcaactc
      841 tgctctgacc ccctgttgct cagctctaca cagctcctgg agttgggttt agaaactgtc
      901 ccttctagaa accttgaatt ttggagggct gacttttgaa aatcttagtg atatacagaa
      961 gcattctctg tccttggggt gtgtgaagta ggtgaaattg cagatcttgg ggtgttctca
     1021 tgactcactc cttggaggga tccctagaga aggaagctgg gatactgggg tgatttcttt
     1081 tgacatcctc ttgtccacca cagctcccga ctatgacaga agccagtggc tgagtgagaa
     1141 gttcaaactg ggcctggact tccccaatgt aggtggaggg aaggggaggt gtggggaagg
     1201 cctagtgctc tcacctcatc tcctggcctg gctgaggggt tggcatcagt gtttctgctt
     1261 gcctgtttca tccctgctgg ctgcacagtg tttctgtgtt gggctgtgtc ctggctctcc
     1321 cactcagtcg acaccgtgct catggagggt ttcctggggc agcacactga gtgccagggc
     1381 catgtctatc ctcacccagg gaagggattc agctacccca taacccatct gaccatccct
     1441 gattgtctat ccagctgccc tacttaattg atgggtcaca caagatcacc cagagcaatg
     1501 ccatcctgcg ctaccttggc cggaagcaca acctttgtga gtggggctga ctgcagggtg
     1561 gggacagaag ccatccctct tggcttggct ggagcaggat gctgagagtg ggtctgtgtt
     1621 gtgtgtgctg caggtgggga gacagaggag gagaggattc gtgtggacgt tttggagaac
     1681 caggctatgg acacccgcct acagttggcc atggtctgct acagccctga ctttgtgagt
     1741 tccaccagcc ctgagttgaa gctggccctg cactcttgct cttgtatcag ctctagcccc
     1801 gtttgccacc acagcctctc agtgctactc atggtacagt gtttgaaatt gccgacagag
     1861 taacccccaa gctcagtttg ccaaatgaaa acttctagtc atttgctcta agatcgtatc
     1921 cagactctcc acagcgacat ttagtccctg ctaggacaga cagagtgtga tccctccagt
     1981 tctagctgct ggttctgtcc tgagctgtgt ctttctgttg ccctggggtc ttgccatgtc
     2041 tgcagccctc atactcacac tatgagaaga cactggggct agggaacact tcctcccaaa
     2101 tggcttccca gagctgtgtc cttgacaccc acagagagaa gcagatgctc ccaataggca
     2161 actcagtcag tcaaaggcct tggatcctcg gctcctgttt cattttgtcc tctcaaattc
     2221 ccctcatttc tttggaacct gtactgaagt cctcactgcc ccagtaggca gaacactacc
     2281 tgtttcctgg gccgtttcag ttgtttgctt ctgcctcatg tgaggtcaga gttcagagtc
     2341 aggtgcctac aactgtctca tgcaaggtgg ttctgataat gatggtggag tccagggaac
     2401 agagctgtat cttgttgggc tgtttccaaa gaacagtcta atcatggtgt tgctctaact
     2461 aaacacgtgg gcctcaaccc agactgaatc tcacgaaggt gactgcttct ctgcacgctg
     2521 gggcctgtac agccctgtga ggccagcctc tgccagggag cctgtgtctg aaggtagtga
     2581 tggttgttct ctgcttcagg agagaaagaa gccagagtac ttagagggtc tccctgagaa
     2641 gatgaagctt tactccgaat tcctgggcaa gcagccatgg tttgcaggga acaaggtaaa
     2701 ggcagcgggt ggggagaagg atttgccatt tcttcccagg tgtcaaattc tagcactcac
     2761 ccttggcttc ctgcagatta cgtatgtgga ttttcttgtt tacgatgtcc ttgatcaaca
     2821 ccgtatattt gaacccaagt gcctggacgc cttcccaaac ctgaaggact tcgtggctcg
     2881 gtttgaggtg atgtcctgac cccgcttcct cttgacctac ttcccttccc cccttccaga
     2941 atgcctttct actccttgaa atggagatga aaatggctag cttctgttga gcatagaact
     3001 gtgttctgct ctttcgtccc ttgcatggag tttcccagca caccctgcat gttgtgtagg
     3061 attatcagct ccttaggatc attttggaag cggattgtaa agactcagtt cctcagggag
     3121 tcagtaccat tggaagggga cgtggttttt ttccagtgtg cttctagctt ccaagaacag
     3181 ggggcaatag atctaccgga taccaaagga aaaaagccat aggttgcaat agagcctgga
     3241 ttttccagcc ctgaagccta tggaaattca ggacatgccc ggaatgtata gggagcacta
     3301 ttcaggattg atgcacagta ccaagataca gtatccatat ctggcctata caattctttg
     3361 ctcagtcaga cccctgagtg gggaagcact gggacccagg gctacagtta gtgtgagtag
     3421 acagctcact gctgttggag gattttatcc tccaacatcc tgtttctttc ctttcctttt
     3481 cctccttggt gacatcttga tgtttgactg tagaatcatt acagtgagac tgtactgcca
     3541 tcgtcatctt ctctagtgtg gcctccgtgt ggcacagttc tgagctcagt acgatgtgga
     3601 aacctgcgtc tctgtccagg catgcagagt ggcaggcacg cctgactatg atgtacatgt
     3661 gatccccaca agccccactt tattagagat ttgggggatc gaggccatag tccaatggga
     3721 atcttagcgt ggggtcttct cctctgtccc tgctgcacac gtgatgcgtt tttccttagt
     3781 tttcattggc ttgccttctg gtccagcctg ctcggctctg gagattgtgt gagaactgtt
     3841 gaacagtgtg gtgggagagt gtgggaggct gcagtccaag gccagccaag cctggcttct
     3901 tgggtaaggc tgccctggaa ctttgaattc atcacagttt atctgggcac cgtactggaa
     3961 agatagcaca cagcacagtg ccattctgta gaatgttctc tagcagggct gagtctaggc
     4021 aggatggaca cactaagtat gcatttagct cccagtgttc tgagtgtaga tttttctgca
     4081 tcaggagaat ggccaaggcc actccattgg ccttgctgtg tcacctatcc ctctgctcat
     4141 tcagtcagga tttcctgagg tactgggtga gatctttgct ctcttccaaa gtacactggc
     4201 atgttactgg tccctttgac ctgtttggtc ctttcccaat gtggaaacgc agggcaagaa
     4261 ggagcctgca ggtaaaaaag aaaagaaaag aaagcgagaa ttgcgtaacc gggtagcaac
     4321 aaggtagctt agggagtgaa ccgagggaat cagaatggag gctgctgagc ccctccctgt
     4381 gtagaccggg atgcagactc tcgctgttcc tgctgagcct gtgtgcctgg cttcctcctg
     4441 gcaggagcac agcactgttt tgcgggattc tgtggagagc tccctcttct tctatacctg
     4501 caccacagct gcagatggac gcagctgaac gcagtgccag tttcccctac atcagaggac
     4561 attaaagcat ccccttacca gagttgtgcc cctgagcaac ccgggctgtg ttggggttct
     4621 tagagatgtc ccagatcctc aattctcgct ttctcctcct cctcccttca gggcctgaag
     4681 aagatatctg actacatgaa gagcggccgc ttcctctcca agccaatctt tgcaaagatg
     4741 gccttttgga acccaaagta gcaccacaaa gtccagacct ggggatactc atgagtgccc
     4801 tgctggctgt gggcctagag catggctctg gcgcccacca catgcagctt tctcctcctt
     4861 tccattccct gttcctccat ctcctcttcc cagcccttgc ctcagtcaag cctcagttcc
     4921 ctggtctctc catttcttca ttagtcccct cccttgtctc tgccctgcat ccaacccttc
     4981 cctcactgat tttcggagga ctgtaccaga cccctgaatc cccagcctgg cctgagagat
     5041 tagatctcac tgtgctgccc tggtccccag gaaggaccca tttgatttgc aataaagtgt
     5101 gaaccacatt tgtccagtgt cctgttttgc tgtctgtgac actcagggct gactgtgttg
     5161 acttggttga ttttgttttg ttgctcgcag gaggagctag agggatggac tctgggctat
     5221 ttga
//