From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Clostridium thermaceticum small subunit corrinoid/iron-sulfur
Date: 22 Dec 92 22:50:54 GMT
Distribution: bionet
Checksum: 04020 4

LOCUS       CLOMTPB      1235 bp ds-DNA             BCT       30-NOV-1992
DEFINITION  Clostridium thermaceticum small subunit corrinoid/iron-sulfur
            protein gene, complete cds.
ACCESSION   L07100
KEYWORDS    methyl transfer protein;
            small subunit corrinoid/iron-sulfur protein.
SOURCE      Clostridium thermoaceticum (strain DMS 521) (library: Ragsdale)
            DNA.
  ORGANISM  Clostridium thermoaceticum
            Prokaryotae; Firmicutes; Endospore-forming rods and cocci;
            Bacillaceae.
REFERENCE   1  (bases 1 to 1235)
  AUTHORS   Lu,W.-P., Schiau,I., Cunningham,J.R. and Ragsdale,S.W.
  TITLE     Sequence and expression of the gene encoding the
            corrinoid/iron-sulfur protein from Clostridium thermoaceticum and
            reconstitution of the recombinant protein to full activity
  JOURNAL   J. Biol. Chem. (1992) In press
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     CDS             101..1072
                     /function="methyl transfer protein"
                     /evidence="EXPERIMENTAL"
                     /note="this is the corrinoid containing subunit of the
                     C/Fe-SP involved in transfer of methyl from
                     methyl-H4folate to carbon monoxide dehydrogenase in CO2
                     ixation by anaerobic bacteria"
                     /product="small subunit corrinoid iron-sulfur protein"
                     /codon_start=1
                     /translation="MAVQILRDRSRAAVQKVVLGATKDQGGTRSHTIVVGGDAALPFH
                     HFEGEIVNRPVIGMEVQDIVPDWPDVLKDPFTDVINEPGRWAQKCVAEYGADLIYLKL
                     DGADPEGANHSVDQCVATVKEVLQAVGVPLVVVGCGDVEKDHEVLEAVAEAAAGENLL
                     LGNAEQENYKSLTAACMVHKHNIIARSPLDINICKQLNILINEMNLPLDHIVIDPSIG
                     GLGYGIEYSFSIMERIRLGALQGDKMLSMPVICTVGYEAWRAKEASAPVSEYPGWGKE
                     TERGILWEAVTATALLQAGAHILLMRHPEAVARVKENIDQLMVSNAY"
BASE COUNT      290 a    332 c    351 g    262 t
ORIGIN      
        1 gtaaacccct ttttgatctg ccggcaactt ccgtatcggt tcaagcagta aaagctatcc
       61 tggcccgatg ccagttttaa gttgaaagga gtgttttaaa atggccgtcc agattttacg
      121 tgatcgtagc cgagctgccg tccagaaagt tgtcctgggc gccaccaaag accagggggg
      181 tacccgcagc cataccatcg tcgtcggtgg cgatgctgcc ctgcctttcc accatttcga
      241 aggagagatt gtcaacaggc cggtaatcgg tatggaagtg caggatatcg tacccgactg
      301 gcccgacgtt ctcaaagatc ccttcaccga tgttattaat gaaccagggc gctgggccca
      361 aaagtgcgta gccgagtatg gtgctgacct tatctacctg aaacttgacg gggccgaccc
      421 cgaaggcgcc aaccattctg tggaccagtg cgtagctact gttaaagagg tcctgcaggc
      481 cgtgggggta cccctggtag tggtaggttg cggcgatgtg gaaaaggacc atgaggtcct
      541 ggaagcagta gccgaggctg ctgccggcga gaatctcctc ctgggtaacg ctgaacagga
      601 aaactataaa tccctaacgg cagcctgcat ggtccacaag cataatatca tcgcccgttc
      661 gcccctggat attaacattt gtaaacaact caacatcctg atcaatgaaa tgaacctgcc
      721 cctggatcat atcgtcatcg acccgtccat cggcggcctg ggttatggta ttgaatactc
      781 cttctcgatt atggaacgca tccgtctggg ggccctgcag ggagataaga tgctctccat
      841 gccggtcatc tgcaccgtag gctatgaggc ctggcgcgcc aaggaagcct cggcaccggt
      901 gagcgaatac ccgggctggg gtaaggaaac cgagcgtggc atcctctggg aagccgttac
      961 cgccactgcc ctgctccagg ccggcgccca catcctcctc atgcgccatc cggaagccgt
     1021 agccagggtg aaggagaata tcgaccagtt aatggtgagc aacgcctatt aaacctggtt
     1081 aatgggccta catgaaaggt ttgtgctggc atttgttgct agcgacctgg acgagggaca
     1141 aaacggcccc gggcaaagca ggggagcctt tcatggaagg catgtgcccc ctcgatgaac
     1201 ggaaaaagtg agttgtggaa gccggggata tatcc
//