From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: African swine fever virus (clones p5RN' and p5RD)
Date: 28 Dec 92 21:25:36 GMT
Distribution: bionet
Checksum: 13815 8

LOCUS       ASVGUANYL    3855 bp ds-DNA             VRL       01-DEC-1992
DEFINITION  African swine fever virus (clones p5RN' and p5RD)
            guanylyltransferase (NP868R) gene, complete cds; D250R gene,
            complete cds.
ACCESSION   L07263
KEYWORDS    guanylyltransferase.
SOURCE      African swine fever virus (strain BA71V) (library: pBR325) DNA.
  ORGANISM  African swine fever virus
            Viridae; ds-DNA nonenveloped viruses; Iridoviridae.
REFERENCE   1  (bases 1 to 3855)
  AUTHORS   Pena,L., Yanez,R.J., Revilla,Y., Vinuela,E. and Salas,M.L.
  TITLE     African swine fever virus guanylyltransferase
  JOURNAL   Virology (1993) In press
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     CDS             38..2644
                     /EC_number="2.7.7.50"
                     /gene="NP868R"
                     /evidence="EXPERIMENTAL"
                     /citation=[1]
                     /note="catalyzes the following reaction in the capping of
                     the viral RNAs:ppRNA + GTP --> GpppRNA + PPi"
                     /product="guanylyltransferase"
                     /codon_start=1
                     /translation="MASLDNLVARYQRCFNDQSLKNSTIELEIRFQQINFLLFKTVYE
                     ALVAQEIPSTISHSIRCIKKVHHENHCREKILPSENLYFKKQPLMFFKFSEPASLGCK
                     VSLAIEQPIRKFILDSSVLVRLKNRTTFRVSELWKIELTIVKQLMGSEVSAKLAAFKT
                     LLFDTPEQQTTKNMMTLINPDDEYLYEIEIEYTGKPESLTAADVIKIKNTVLTLISPN
                     HLMLTAYHQAIEFIASHILSSEILLARIKSGKWGLKRLLPQVKSMTKADYMKFYPPVG
                     YYVTDKADGIRGIAVIQDTQIYVVADQLYSLGTTGIEPLKPTILDGEFMPEKKEFYGF
                     DVIMYEGNLLTQQGFETRIESLSKGIKVLQAFNIKAEMKPFISLTSADPNVLLKNFES
                     IFKKKTRPYSIDGIILVEPGNSYLNTNTFKWKPTWDNTLDFLVRKCPESLNVPEYAPK
                     KGFSLHLLFVGISGELFKKLALNWCPGYTKLFPVTQRNQNYFPVQFQPSDFPLAFLYY
                     HPDTSSFSNIDGKVLEMRCLKREINYVRWEIVKIREDRQQDLKTGGYFGNDFKTAELT
                     WLNYMDPFSFEELAKGPSGMYFAGAKTGIYRAQTALISFIKQEIIQKISHQSWVIDLG
                     IGKGQDLGRYLDAGVRHLVGIDKDQTALAELVYRKFSHATTRQHKHATNIYVLHQDLA
                     EPAKEISEKVHQIYGFPKEGASSIVSNLFIHYLMKNTQQVENLAVLCHKLLQPGGMVW
                     FTTMLGEQVLELLHENRIELNEVWEARENEVVKFAIKRLFKEDILQETGQEIGVLLPF
                     SNGDFYNEYLVNTAFLIKIFKHHGFSLVQKQSFKDWIPEFQNFSKSLYKILTEADKTW
                     TSLFGFICLRKN"
     CDS             2701..3453
                     /standard_name="D250R protein"
                     /gene="D250R"
                     /citation=[1]
                     /codon_start=1
                     /translation="MDTAMQLKTSIGLITCRMNTQNNQIETILVQKRYSLAFSEFIHC
                     HYSINANQGHLIKMFNNMTINERLLVKTLDFDRMWYHIWIETPVYELYHKKYQKFRKN
                     WLLPDNGKKLISLINQAKGSGTLLWEIPKGKPKEDESDLTCAIREFEEETGITREYYQ
                     ILPEFKKSMSYFDGKTEYKHIYFLAMLCKSLEEPNMNLSLQYENRIAEISKISWQNME
                     AVRFISKRQSFNLEPMIGPAFNFIKNYLRYKH"
BASE COUNT     1236 a    790 c    731 g   1098 t
ORIGIN      
        1 atttttttat aatttgaata gttattccaa attcaatatg gcttctttag ataatttagt
       61 ggcacgatat cagaggtgct ttaatgacca gtctcttaaa aatagtacta ttgaacttga
      121 aatacgtttt caacagataa attttttatt attcaaaacc gtatatgagg cacttgtggc
      181 acaagagatc cctagcacca tctcccacag catccgctgc atcaaaaaag ttcaccatga
      241 aaaccactgc cgggaaaaaa ttttgccgtc ggaaaatctt tacttcaaaa aacagcctct
      301 catgtttttt aagttttcag agcctgcatc tctgggctgt aaggtctcgc tggccatcga
      361 gcagcccatt cgtaaattta tcttggactc ctccgttctc gttcggctca aaaatcgtac
      421 gacctttcgg gtatctgaac tttggaaaat agagcttacc attgtaaagc agctgatggg
      481 aagcgaggtc tctgcaaaac ttgccgcttt caaaacgctt ctgtttgaca ccccagagca
      541 acaaacgaca aaaaatatga tgacgttaat aaacccagat gacgaatatc tttacgaaat
      601 agaaatagag tatacaggaa agcccgaatc cctaacggcg gcagatgtta taaaaattaa
      661 aaacacggtg ttgacactta tttctccgaa ccatttaatg ctaacagcct accaccaggc
      721 cattgaattc attgcctccc atatactgtc ctcagaaatc cttcttgctc gtattaagag
      781 cgggaagtgg gggcttaaac gcctcctccc ccaggtgaaa tccatgacca aagcggatta
      841 catgaaattt tatccgcccg ttggctacta tgtaacggac aaagcagatg gaattagagg
      901 catcgccgtc attcaggaca cgcaaattta tgtggttgca gaccagctat acagcctagg
      961 taccaccggc attgaacccc ttaaaccaac cattttggat ggtgaattta tgcctgaaaa
     1021 aaaagaattt tatgggtttg acgtcatcat gtatgagggc aatctgttga cgcaacaggg
     1081 gtttgaaaca agaattgagt ctttaagcaa gggcattaaa gtcttacaag cgtttaacat
     1141 aaaagcagaa atgaagccct ttatttcgct aacaagtgca gatcccaacg tgctcctcaa
     1201 aaactttgaa agcattttta agaaaaaaac tcgcccatat tctattgatg gcatcatttt
     1261 agtagaacct ggcaattctt atctaaatac aaatactttt aagtggaagc ccacctggga
     1321 taacacatta gactttttgg tgcgaaaatg tccggagagt ttaaacgtgc cagagtacgc
     1381 gcccaaaaaa gggttttccc tgcatctact atttgtaggc atctccggag agctttttaa
     1441 aaaattagcg ctaaattggt gtccaggata tacgaaacta ttccccgtta cacagcgcaa
     1501 ccaaaactac tttccagtac agtttcagcc atcggatttt ccactggcat ttctttatta
     1561 ccacccagat acctcgtcat tttctaatat agatggaaag gtccttgaaa tgcgttgtct
     1621 taagagagaa attaattacg tcagatggga aattgtaaaa atccgggagg ataggcagca
     1681 ggatcttaaa accggcgggt attttggcaa tgatttcaaa acagccgaac tcacatggct
     1741 taactatatg gatccctttt cctttgagga actggcaaag ggcccttctg gaatgtactt
     1801 cgccggtgcc aaaaccggca tataccgcgc tcaaacagca cttatttcct ttattaaaca
     1861 agaaatcatc caaaaaataa gtcaccaatc ctgggttatc gatcttggaa taggaaaagg
     1921 gcaggaccta ggacgttacc tggacgcagg ggtaaggcat cttgttggaa tcgataagga
     1981 ccaaaccgcg ctcgcggagc ttgtttatcg aaaattttcg catgctacaa cccgacagca
     2041 caagcacgct accaacattt acgtgttgca tcaagacctc gcagagcctg cgaaggaaat
     2101 cagcgaaaag gtacaccaaa tttacgggtt tcccaaggag ggagcttctt ccattgttag
     2161 caacctgttt attcactatc ttatgaaaaa cacgcagcag gtggaaaacc tggccgttct
     2221 gtgccataag cttcttcagc cggggggaat ggtgtggttt accaccatgt tgggagaaca
     2281 ggtcttagaa ttacttcatg aaaatagaat agagctcaat gaagtatggg aggcacgtga
     2341 aaacgaggtg gtcaaatttg ctattaaacg tctctttaaa gaggatatat tacaggaaac
     2401 tgggcaagaa attggagtcc tgttaccctt cagcaatggc gacttctaca atgaatatct
     2461 tgtgaacaca gcgtttttaa ttaaaatatt taaacatcac ggcttttccc tagttcaaaa
     2521 gcagtccttt aaggactgga ttccagaatt tcaaaacttt agtaaaagtt tgtataaaat
     2581 tcttacagaa gccgataaaa cttggacaag cctttttggg tttatttgtc tgcgcaaaaa
     2641 ttaaatattt tttcataaga agtactaccc aggttttaaa gaaatagcta aaaatatcat
     2701 atggatactg ccatgcagct taaaacgtct attggtttaa ttacttgtcg tatgaacacc
     2761 caaaataacc aaatagaaac tattctggtt caaaaacgtt acagccttgc tttttcagaa
     2821 tttattcatt gtcattactc gataaatgct aatcaaggtc atctgattaa aatgtttaat
     2881 aacatgacaa ttaatgaacg actgcttgtc aaaacactgg attttgaccg catgtggtat
     2941 catatttgga ttgaaactcc agtctacgaa ctataccaca aaaaatacca aaaatttagg
     3001 aaaaattggc ttctcccaga taatgggaaa aagcttattt cattaatcaa ccaagcaaag
     3061 ggctcaggaa cacttctatg ggaaattcct aagggtaagc cgaaggaaga tgagtcggac
     3121 cttacctgtg ccatacggga gtttgaagaa gaaaccggga ttacccgcga atattaccag
     3181 attctcccag agtttaaaaa atctatgtca tactttgacg gtaaaacaga atataagcat
     3241 atctacttcc ttgcaatgtt atgtaagtcg ttggaggaac ccaatatgaa tctttcttta
     3301 caatacgaaa atcggattgc cgaaatttct aaaatttctt ggcaaaatat ggaggctgta
     3361 cgttttatta gcaaacgcca gtcatttaac ctggagccta tgatcgggcc tgcatttaat
     3421 tttattaaaa actatttacg atataagcac taggatgccg cattaaaatg ccgcataagg
     3481 taatacacta ggaatgccgc acacgcacaa gaatacaatg tcgccggaga tttattatct
     3541 agtacacgtt ttatgtatgt acaatccgcc ttcatttaat atattgagcg gatgtactat
     3601 gtatttattt taacaaaaaa acattatttt tttttaatct tcatcatcta tttttataaa
     3661 ctcagtaata tcaaaagtag cttgtggggt ttcagagggt tcaccttggt tatcctccgt
     3721 gaggataaca tgttcttcag gttcgtcgtc actggagaac ccatcattta attcctcttc
     3781 actcaacatc tgtaaaaaat cttccaagct ttcgctatcg ttaaaatcct catcatccat
     3841 aagaataatg gtacc
//