From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Clostridium thermohydrosulfuricum cyclodextrinase (CDase) gene,
Date: 22 Aug 92 10:00:22 GMT
Distribution: bionet
Checksum: 42608     6

LOCUS       CLOCDASE     2455 bp ds-DNA             BCT       22-AUG-1992
DEFINITION  Clostridium thermohydrosulfuricum cyclodextrinase (CDase) gene,
            complete cds.
ACCESSION   M88602
KEYWORDS    CDase gene; cyclodextrinase; cyclomaltodextrin hydrolase.
SOURCE      Clostridium thermohydrosulfuricum (strain 39E) DNA.
  ORGANISM  Clostridium thermohydrosulfuricum
            Prokaryotae; Firmicutes; Endospore-forming rods and cocci;
            Bacillaceae.
REFERENCE   1  (bases 1 to 2455)
  AUTHORS   Podkovyrov,S.M. and Zeikus,J.G.
  TITLE     Structure of the gene encoding cyclomaltodextrinase from
            Clostridium thermohydrosulfuicum 39E and characterization of the
            enzyme purified from Escherichia coli
  JOURNAL   J. Bacteriol. 174, 5400-5405 (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     -35_signal      307..312
                     /gene="CDase"
     -10_signal      330..335
                     /gene="CDase"
     RBS             407..412
                     /gene="CDase"
     CDS             420..2144
                     /function="degrades cyclodextrins"
                     /EC_number="3.2.1.54"
                     /standard_name="cyclomaltodextrin hydrolase, decycling"
                     /product="cyclodextrinase"
                     /gene="CDase"
                     /evidence=EXPERIMENTAL
                     /codon_start=1
                     /translation="MIKEAIFHKSDVPYAYPLNENQLKIVLRTAVFDVDRVYVLYKDR
                     YDWLGKFKIKPMVLTHTNELFDYYETTLELNKKFVYFFYLVSDGGEKLYYTEAGFYKK
                     RPENHFWGFFHYPYIGEKDVFFAPEWTSDCMVYQIFPERFNNGDKSNDPENVKPWGEK
                     PTADSFFGGDLQGIIDKIDYLKDLGINAIYLTPIFLSHSTHKYDTTDYYTIDPHFGDT
                     QKARELVQKCHDNGIKVIFDAVFNHCGYDFFAFQDVIKNGKKSKYWDWFNIYEWPIKT
                     HGKPSYEAFADTVWRMPKLMTKNPEVQKYLLEVAEYWIKEVDIDGWRLDVANEIDHHF
                     WRKFREVVKAAKPEAIIVGEVWHDASPWLRGDQFDSVMNYPFRNAVVDFFAKRKISAS
                     RFNTMITEQLMRHMDSVNRVMFNLIGSHDTERFLTLANGMVARMKLALVFQFTFVGIP
                     YIYYGDEVGMVGDYDPDCRRCMIWEEEKQNKSIFNFYKKLISIRRENEELKYGSFCTL
                     YAIGRVFAFKREYKGKSIIVVLNNSSKQEVIFLNEVEGKEDILKMKELKRSGNLLYLQ
                     PNSAYILK"
     terminator      2179..2215
                     /gene="CDase"
BASE COUNT      908 a    309 c    518 g    720 t
ORIGIN
        1 ggacttgcac caattccaca acaaaatcaa agtgaagctt caacaatttc ggaagacaaa
       61 acaaatcttc cacaggaaag caatgtttct caacctatag taaagacgtc gattgaaaaa
      121 gtagcagaga cttctaaaaa aatagaatat aaagttgttc cctttggaga aagttataaa
      181 gtagaaataa gcgtacctgg cgaaaaatgt tggtttagtg taaaagtaga tggaaatgtc
      241 gtttatgaag gccttatgac aaaggatatg tctaaaattt ttgatgtaaa agatagtatt
      301 actatattga tgggatatcc gccagctgtt aaaataactg tagacggtga ggagctacca
      361 acagtacata caccatcacc ggttactata aatattcatc catagaaagg agaaaaaaga
      421 tgataaaaga agctatattc cataaaagcg atgtgcctta cgcatatcca ctgaatgaaa
      481 atcaattaaa gatagtacta aggactgctg tatttgatgt agatagggtg tatgttttat
      541 acaaagacag atacgattgg ctgggaaaat tcaaaataaa accaatggta ttaactcata
      601 ccaacgagtt atttgattat tatgagacta ctttagagct taataaaaaa tttgtgtatt
      661 ttttctattt ggtatctgat gggggcgaga agttatatta tacagaagca ggattttaca
      721 aaaaaaggcc agaaaatcat ttttggggat ttttccatta cccgtatata ggagaaaagg
      781 atgtcttttt tgctccagag tggacaagcg attgcatggt gtatcagata tttcctgaaa
      841 ggtttaataa cggtgataaa tcaaatgacc ctgaaaatgt aaagccctgg ggtgaaaaac
      901 ctactgcaga ttctttcttt ggaggagatt tgcaaggaat aatagataaa atagattatt
      961 taaaggattt gggtataaat gccatttatt taacccccat atttttatcg cattccaccc
     1021 acaaatatga tactactgat tattatacaa ttgaccccca ctttggtgat acgcaaaaag
     1081 cgagagaact tgtgcaaaaa tgtcatgaca atggcataaa agttatattt gatgccgttt
     1141 ttaatcactg cggatatgac ttttttgcat ttcaggatgt tattaaaaat ggtaaaaaat
     1201 caaaatactg ggattggttt aatatatacg aatggccaat taaaactcat ggaaaacctt
     1261 cctatgaagc ttttgcagat actgtatgga gaatgccaaa acttatgaca aaaaatccag
     1321 aagtacagaa gtatttattg gaagtagcgg aatattggat taaagaagtg gacatagacg
     1381 gctggaggct ggatgttgct aatgagatag accaccattt ttggagaaag tttagagaag
     1441 ttgtaaaggc agctaaaccg gaggccataa ttgtaggaga ggtttggcat gatgcttctc
     1501 cttggcttag aggggatcag tttgacagtg taatgaacta tccttttaga aatgcggttg
     1561 tggacttttt tgcaaaaagg aaaataagtg cttctcgctt taatacaatg attacagagc
     1621 aacttatgag gcatatggat agtgtaaata gggttatgtt taatttaata ggaagtcatg
     1681 atactgagag gtttttgact ttagctaatg gcatggttgc gagaatgaag ttagcattag
     1741 tgtttcaatt tacctttgtt ggaatcccat atatttacta tggggacgag gtaggaatgg
     1801 tgggagatta tgaccctgat tgcagaagat gcatgatatg ggaggaggaa aagcaaaata
     1861 aaagtatttt taatttctat aaaaagttga tttctataag gagagaaaat gaggagctca
     1921 aatacggaag tttctgtact ttatatgcta taggaagagt gtttgctttt aagagggaat
     1981 acaaaggtaa atcaataatt gttgtactaa ataacagtag caagcaggaa gtgatatttt
     2041 tgaatgaagt agaaggaaaa gaagatattt taaagatgaa ggaattaaaa agaagtggaa
     2101 atctccttta cttgcaacct aactctgcct atattttaaa gtaaccaatc tgagtttaag
     2161 tgaataatat gaagatagaa aaatataggg gaattaaaat tcccctatat tttttaaaaa
     2221 aaacaggggt gatggtatga aagatacatc ccgctatggc agagattatc aatattataa
     2281 ggattatgag agattatatg gaagttacga cattacaaag cttatcacat taaaaaaatt
     2341 agttgaagca agtgaatatg aagagtggaa agattacaaa gagttcaagg aatggtgtaa
     2401 ttctgaagaa agacataatt ggaaaaattc acctaaatat attgaatggg aaaag
//