From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: E.coli periplasmic phosphoanhydride phosphohydrolase (appA) gene,
Date: 8 Jan 93 05:46:54 GMT
Distribution: bionet
Checksum: 28611 5

LOCUS       ECOACPHF     1901 bp ds-DNA             BCT       07-JAN-1993
DEFINITION  E.coli periplasmic phosphoanhydride phosphohydrolase (appA) gene,
            mutant region.
ACCESSION   L03375
KEYWORDS    acid phosphatase; periplasmic phosphoanhydride phosphohydrolase.
SOURCE      Escherichia coli DNA.
  ORGANISM  Escherichia coli
            Prokaryotae; Gram-negative facultatively anaerobic rods;
            Enterobacteriaceae.
REFERENCE   1  (sites)
  AUTHORS   Dassa,J., Marck,C. and Boquet,P.-L.L.
  TITLE     The complete nucleotide sequence of the Escherichia coli gene appA
            reveals significant homology between pH 2.5 acid phosphatase and
            glucose-1-phosphatase
  JOURNAL   J. Bacteriol. 172, 5497-5500 (1990)
  STANDARD  full automatic
REFERENCE   2  (bases 1 to 1901)
  AUTHORS   Ostanin,K., Harms,E.H., Stevis,P.E., Kuciel,R., Zhou,M.-M. and Van
            Etten,R.L.
  TITLE     Overexpression, site-directed mutagenesis, and mechanism of
            Escherichia coli acid phosphatase
  JOURNAL   J. Biol. Chem. (1992) In press
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     mutation        299..301
                     /phenotype="H303A mutant"
                     /gene="appA"
                     /citation=[1]
     CDS             188..1486
                     /standard_name="acid phosphatase"
                     /gene="appA"
                     /citation=[1]
                     /product="periplasmic phosphoanhydride phosphohydrolase"
                     /codon_start=1
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESVVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     GQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILSRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLKREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIKTALTPHPPQKQAYGVTLPTSVLFIAGADTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
BASE COUNT      473 a    499 c    500 g    428 t      1 others
ORIGIN      
        1 taaggagcag aaacaatgtg gtatttactt tggttcgtcg gcattttgtt gatgtgttcg
       61 ctctccaccc ttgtgttggt atggctggac ccgcgtctga aaagttaacg aacgtaggcc
      121 tgatgcggcg cattagcatc gcatcaggca atcaataatg tcagatatga aaagcggaaa
      181 catatcgatg aaagcgatct taatcccatt tttatctctt ctgattccgt taaccccgca
      241 atctgcattc gctcagagtg agccggagct gaagctggaa agtgtggtga ttgtcagtcg
      301 tcatggtgtg cgtgctccaa ccaaggccac gcaactgatg caggatgtca ccccagacgc
      361 atggccaacc tggccggtaa aactgggttg gctgacaccg cgnggtggtg agctaatcgc
      421 ctatctcgga cattaccaac gccagcgtct ggtagccgac ggattgctgg cgaaaaaggg
      481 ctgcccgcag tctggtcagg tcgcgattat tgctgatgtc gacgagcgta cccgtaaaac
      541 aggcgaagcc ttcgccgccg ggctggcacc tgactgtgca ataaccgtac atacccaggc
      601 agatacgtcc agtcccgatc cgttatttaa tcctctaaaa actggcgttt gccaactgga
      661 taacgcgaac gtgactgacg cgatcctcag cagggcagga gggtcaattg ctgactttac
      721 cgggcatcgg caaacggcgt ttcgcgaact ggaacgggtg cttaattttc cgcaatcaaa
      781 cttgtgcctt aaacgtgaga aacaggacga aagctgttca ttaacgcagg cattaccatc
      841 ggaactcaag gtgagcgccg acaatgtctc attaaccggt gcggtaagcc tcgcatcaat
      901 gctgacggag atatttctcc tgcaacaagc acagggaatg ccggagccgg ggtggggaag
      961 gatcaccgat tcacaccagt ggaacacctt gctaagtttg cataacgcgc aattttattt
     1021 gctacaacgc acgccagagg ttgcccgcag ccgcgccacc ccgttattag atttgatcaa
     1081 gacagcgttg acgccccatc caccgcaaaa acaggcgtat ggtgtgacat tacccacttc
     1141 agtgctgttt atcgccggag ccgatactaa tctggcaaat ctcggcggcg cactggagct
     1201 caactggacg cttcccggtc agccggataa cacgccgcca ggtggtgaac tggtgtttga
     1261 acgctggcgt cggctaagcg ataacagcca gtggattcag gtttcgctgg tcttccagac
     1321 tttacagcag atgcgtgata aaacgccgct gtcattaaat acgccgcccg gagaggtgaa
     1381 actgaccctg gcaggatgtg aagagcgaaa tgcgcagggc atgtgttcgt tggcaggttt
     1441 tacgcaaatc gtgaatgaag cacgcatacc ggcgtgcagt ttgtaatgca taaaaaagag
     1501 cattcagtta cctgaatgct ctgaggctga tgacaaacga agaactgtct aatgcgtaga
     1561 ccggaaaagg cgttcacgcc gcatccggcc actttcagtt ttcctctttc tcggagtaac
     1621 tataaccgta atagttatag ccgtaactgt aagcggtgct ggcgcgttta atcacaccat
     1681 tgaggatagc gcctttaata ttgacgcctg cctgttccag acgctgcatt gacaaactca
     1741 cctctttggc ggtgttcaag ccaaaacgcg caaccagcag gctggtgcca acagaacgcc
     1801 ccacgaccgc ggcatcactc accgccagca tcggcggcgt atcgacaatc accagatcgt
     1861 aatggtcgtt cgcccattcc agtaattgac gcatccgatc g
//