From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: E.coli lipoic acid biosynthesis lipA, lipB, and ORFs 1, 2 and 3
Date: 31 Dec 92 01:44:27 GMT
Distribution: bionet
Checksum: 35283 11

LOCUS       ECOLIPAB     4534 bp ds-DNA             BCT       18-DEC-1992
DEFINITION  E.coli lipoic acid biosynthesis lipA, lipB, and ORFs 1, 2 and 3
            genes, complete cds; dacA gene, 3' end.
ACCESSION   L07636
KEYWORDS    D-alanine carboxypeptidase; dacA gene; lip genes; lipA gene;
            lipB gene; lipoic acid biosynthesis.
SOURCE      Escherichia coli (sub_strain W3110, strain K-12) (library: Kohara
            Bank miniset) DNA.
  ORGANISM  Escherichia coli
            Prokaryotae; Gram-negative facultatively anaerobic rods;
            Enterobacteriaceae.
REFERENCE   1  (sites)
  AUTHORS   Broome-Smith,J.K., Ioannidis,I., Edelman,A. and Spratt,B.G.
  TITLE     Nucleotide sequences of the penicillin-binding protein 5 and 6
            genes of Escherichia coli
  JOURNAL   Nucleic Acids Res. 16, 1617-1617 (1988)
  STANDARD  full automatic
REFERENCE   2  (sites)
  AUTHORS   Vanden Boom,T.J., Reed,K.E. and Cronan,J.E.Jr.
  TITLE     Lipoic acid metabolism in E. coli: isolation of null mutants
            defective in lipoic acid biosynthesis, molecular cloning and
            characterization of the E. coli lip locus, and identification of
            the lipoylated protein of the glycine cleavage system
  JOURNAL   J. Bacteriol. 173, 6411-6420 (1991)
  STANDARD  full automatic
REFERENCE   3  (sites)
  AUTHORS   Hayden,M.A., Huang,I., Bussiere,D.E. and Ashley,G.W.
  TITLE     The biosynthesis of lipoic acid: Cloning of LIP, a lipoate
            biosynthetic locus of Escherichia coli
  JOURNAL   J. Biol. Chem. 267, 9512-9515 (1992)
  STANDARD  full automatic
REFERENCE   4  (bases 1 to 4534)
  AUTHORS   Reed,K.E. and Cronan,J.E.Jr.
  TITLE     Lipoic acid metabolism in E. coli: sequencing and functional
            characterization of the lipA and lipB genes
  JOURNAL   J. Bacteriol. (1993) In press
  STANDARD  full automatic
COMMENT     [1] refers to bp 1..543 [3] refers to bp 3034..4115 Reed et al.,
            [J. Bacteriol. ??, ??-?? (1993)] refers to bp 1000..1700 and bp
            2980-4149.
FEATURES             Location/Qualifiers
     misc_feature    1707..2433
                     /note="sequence from this region has not been verified on
                     bothstrands"
     misc_feature    4121..4534
                     /note="sequence from this region has not been verified
                     from bothstrands"
     CDS             <1..543
                     /gene="dacA"
                     /citation=[1]
                     /product="D-alanine carboxypeptidase"
                     /codon_start=1
                     /translation="QLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISA
                     VMGGRTFKGREAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDV
                     YLTIPRGRMKDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPE
                     GNFFGKIIDYIKLMFHHWFG"
     CDS             653..916
                     /note="ORF 1"
                     /codon_start=1
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     CDS             1084..1659
                     /gene="lipB"
                     /citation=[4]
                     /product="LIPB protein"
                     /codon_start=1
                     /translation="MHEFTDTRDDDTLDEIWLVEHYPVFTQGQAGKAEHILMPGDIPV
                     IQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELVTLLEQTVVNTLAELGIEAHPRA
                     DAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDLSPFLRINPCGYAGMEMAKISQW
                     KPEATTNNIAPRLLENILALLNNPDFEYITA"
     CDS             complement(1932..2192)
                     /note="ORF 3"
                     /codon_start=1
                     /translation="MHRKCGRSDTLAFANKQWVREYNAQFLNRLTDCRGGQIKSTFAA
                     LTIPLCTYTASKIVRRLRSIFRKVVNICGLPSDDFLLKQGSI"
     CDS             2197..2871
                     /note="ORF 2"
                     /codon_start=1
                     /translation="MSISVRGLESILGALDIEGSYDKQRTITIATTPSVGALVLPVIY
                     RAIKTHYPQLLLRNPPISDAENQLSQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICR
                     EGNPLLSLEDDRETIDNAAHVLLLPEEQNFSGLRQRVQEMFPDRQINFTSYNILTIAA
                     LVANSDMLAIIPSRFYNLFSRCWPLEKLPFPSLNEEQIDFSIHYKFSLRDPILHGVID
                     VIRNAF"
     CDS             3079..4044
                     /gene="lipA"
                     /citation=[3]
                     /citation=[4]
                     /product="LIPA protein"
                     /codon_start=1
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALVRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
BASE COUNT     1187 a   1106 c   1025 g   1196 t     20 others
ORIGIN      14.9 1990 genetic map
        1 cagctgaacc gtaacggcct gttatgggat aacagcctga atgtcgacgg catcaaaacc
       61 ggacacactg acaaagcagg ttacaacctt gttgcttctg cgactgaagg ccagatgcgc
      121 ttgatttctg cggtaatggg cggacgtact tttaaaggcc gtgaagccga aagtaaaaaa
      181 ctgctaacct ggggcttccg tttctttgaa accgttaacc cactgaaagt aggtaaagag
      241 ttcgcctctg aaccggtttg gtttggtgat tctgatcgcg cttcgttagg ggttgataaa
      301 gacgtgtacc tgaccattcc gcgtggtcgc atgaaagatc tgaaagccag ctatgtgctg
      361 aacagcagtg aattgcatgc gccgctgcaa aagaatcagg tcgtcggaac tatcaacttc
      421 cagcttgatg gcaaaacgat cgagcaacgc ccgctggttg tgttgcaaga aatcccggaa
      481 ggtaacttct tcggcaaaat cattgattac attaaattaa tgttccatca ctggtttggt
      541 taaaaattaa acacttgaaa gtgtaatttc cgtccccata tactaagcat cagtaaaaaa
      601 ctcccgcctt ctggcgggag ttgctattta attacgttac gccggagctg acatgaaaac
      661 caaacttaac gaactgcttg aattccctac tccttttact tacaaagtta tggggcaggc
      721 gttacctgag ctggttgatc aggtggttga agtggtacag cgccatgcgc caggtgacta
      781 caccccaacg gtaaaaccaa gcagcaaagg caactaccac tcggtatcta tcactatcaa
      841 cgccactcat atcgagcagg ttgaaacact gtatgaagaa ctgggcaaaa tcgatattgt
      901 ccgcatggtt ctgtaactcg cttctccgtt acccggctcc tggtcgggta actcccctct
      961 cctcgctgtg atatactttt ccccccactt ttactcattc tccacggaga tgccgttttg
     1021 tatcaggata aaattcttgt ccgccagctc ggtcttcagc cttacgagcc aatctcccag
     1081 gctatgcatg aattcaccga tacccgcgat gatgataccc ttgatgaaat ctggctggtc
     1141 gagcactatc cggtattcac ccaaggtcag gcaggaaaag cggagcacat tttaatgccg
     1201 ggtgatattc cggtgatcca gagcgatcgc ggtgggcagg tgacttatca cgggccgggg
     1261 caacaggtga tgtatgtgtt gcttaacctg aaacgccgta aactcggtgt gcgtgaactg
     1321 gtgaccttgc ttgagcaaac agtggtgaat accctggctg aactgggtat agaagcgcat
     1381 cctcgggctg acgcgccagg tgtctatgtt ggggaaaaga aaatttgctc actgggttta
     1441 cgtattcgac gcggttgttc attccacggt ctggcattaa acgtcaatat ggatctttca
     1501 ccatttttac gtattaatcc ttgtgggtat gccggaatgg aaatggctaa aatatcacaa
     1561 tggaaacccg aagcgacgac taataatatt gctccacgtt tactggaaaa tattttagcg
     1621 ctactaaaca atccggactt cgaatatatt accgcttaat tccatacatc aatggcccaa
     1681 tttacactgg gtcattactc ataatatctg ctaatgcttt acatctcctt cgcgtttgta
     1741 tattttcttt atcacttgca gaaatgcggt gccacgcctt gctccgtaac tcaccccatt
     1801 gattaaccta ttgaaatagt tgattatggt gagcaaaagc gttccgttcc aattagacat
     1861 aagattattg taatttttta aatattattt ttccatgaat aaaattggag taagtgcgtg
     1921 gatagtaata atcaaattga accctgttta agcagaaagt cgtcagaagg taaaccacaa
     1981 atatttacaa ccttacgaaa tattgatctt aaccttctga ctatttttga agctgtatat
     2041 gtacataaag ggatcgttaa tgcagcgaaa gtggacttaa tctgaccccc tcggcaatca
     2101 gtcagtctat tcagaaactg cgcgttatat tccctgaccc attgtttatt cgcaaaggcc
     2161 agggtgtcac tcctaccgca tttgcgatgc atctacatga gtatatcagt caggggcctt
     2221 gagtccattc ttggcgcgct ggatatcgaa ggaagctatg ataagcaacg aacgataact
     2281 attgcgacca ctccctcggt cggagcactg gtccttcctg tcatctatcg ggcgattaaa
     2341 actcactatc cgcagctttt actgcgcaac ccgcccatca gcgacgcgga aaaccaactc
     2401 agtcagtttc aaaccgatct catcatcgat aacatgtttt gcaccaatcg tacggtgcaa
     2461 catcatgttc tgttcaccga caatatggtg ttaatttgcc gcgagggaaa tccactactc
     2521 tctttagaag atgacagaga gactatcgac aacgctgcgc atgtacttct gttaccggaa
     2581 gagcaaaatt tcagcggtct gcggcagaga gttcaagaaa tgtttccgga ccggcaaatc
     2641 aatttcacca gctacaacat tttgacaatc gctgcactgg ttgccaacag tgacatgtta
     2701 gcgattattc ccagccgttt ttataacctg tttagccgct gctggccgct ggaaaaactg
     2761 ccttttccgt ccttaaatga ggagcaaata gatttctcta tccactacaa attcagcctg
     2821 cgtgatccca tcctgcacgg agtaatagat gttatccgta atgcattttg aaaaagtatt
     2881 gttacagcca gatcacagcg cagaacgata aaatggcaca atccaacaac aattttatct
     2941 tttcgcgacg ctttggctgc tttcaggtcc gtttcaatga tatactgcca gtcgttaatt
     3001 caaaaatagt tgataattac aacaatctat tgaattgaaa cgctttcctt cgtaattcgc
     3061 aactggaaca cgcacgctat gagtaaaccc attgtgatgg aacgcggtgt taaataccgc
     3121 gatgccgata agatggccct tatcccggtt aaaaacgtgg caacagagcg cgaagccctc
     3181 gtgcgcaagc cggaatggat gaaaatcaag cttccagcgg actctacacg tatccagggc
     3241 atcaaagccg caatgcgcaa aaatggcctg cattctgtct gcgaggaagc ctcctgccct
     3301 aacctggcgg aatgcttcaa ccacggcaca gcaacgttta tgatcctcgg cgctatttgt
     3361 acccgccgtt gtccgttctg tgatgttgcc cacggtcgcc cggtagctcc tgatgccaat
     3421 gaaccagtga aactggcgca gaccattgcc gatatggcgc tgcgttatgt ggttatcacc
     3481 tccgttgacc gtgatgacct gcgcgatggc ggtgcccagc actttgcgga ttgcattact
     3541 gccattcggg aaaaaagccc gcaaatcaaa attgaaactc tggtgccgga tttccgcggt
     3601 cgtatggatc gtgctctgga tattctgact gcaacgccac cagatgtgtt caaccataac
     3661 ctggaaaacg taccgcgtat ttaccgtcag gtacggcctg gtgcagatta caactggtcg
     3721 ctgaagctgc tggaacgctt taaagaagcg catccggaaa tcccgaccaa gtctggtctg
     3781 atggtgggac tgggtgaaac caatgaagaa attattgagg taatgcgcga cctgcgccgt
     3841 catggtgtga cgatgttaac gctggggcaa tatttgcagc caagccgcca tcacctgccg
     3901 gttcaacgtt acgttagccc ggatgagttc gacgaaatga aagccgaagc gctggcgatg
     3961 ggctttaccc atgctgcatg cggtccgttt gtccgctctt cttaccacgc cgatttgcag
     4021 gcgaaaggga tggaagttaa gtaatcctgt aataattact tacatctgtc tgataaaaaa
     4081 cccgcttttt gaagcggttt tttgtatcga acaagatatt gagggagcgt cctgctcgcc
     4141 acgtcactct ttatgagaga gcttttcagc ctgaagatca acgtctgcgc cttttttcgc
     4201 cgcagcatcg tcatcattca tcgccttctt gaacctttaa ttggccgctc cnnnnnnnnn
     4261 nnnnnnnnnn nccgaccttc atcccttcca cctccagtaa cggacgagtt tcattaccac
     4321 gatcaacacg gcgtgattcc tgaatggcaa atgcatcata gagatgcagt ttggcataac
     4381 gggcgacgat gtttcctgcc tgaagtgcca ccagcatatt ccatgcggcc cccggcgttg
     4441 aaggaacatg aatcgtcaga attgtcgtca tcatgttacg tttactttct cgccgtaaag
     4501 tccgaggaat tctagaagct tctgcagacg cgtc
//