From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Erwinia herbicola dycopene cyclase (crtY), phytoene dehydrogenase
Date: 7 Apr 92 10:01:43 GMT
Distribution: bionet
Checksum: 59601    14

LOCUS       ERWCRTA      6965 bp ds-DNA             BCT       07-APR-1992
DEFINITION  Erwinia herbicola dycopene cyclase (crtY), phytoene dehydrogenase
            (crtI), prephytoene pyrophosphate synthase (crtB), crtE, crtX, and
            crtI genes, complete cds.
ACCESSION   M90698
KEYWORDS    dycopene cyclase; phytoene dehydrogenase;
            prephytoene pyrophosphate synthase.
SOURCE      Erwinia herbicola DNA.
  ORGANISM  Erwinia herbicola
            Prokaryota; Bacteria; Gram-negative facultatively anaerobic rods;
            Enterobacteriaceae.
REFERENCE   1  (bases 1 to 6965)
  AUTHORS   To,K.-Y., Lai,E.-M., Lin,T.-P., Lee,L.-Y., Chang,Y.-S. and
            Liu,S.-T.
  TITLE     Structure analysis of the Erwinia herbicola carotenoid synthesis
            genes
  JOURNAL   Unpublished (1992)
  STANDARD  full staff_entry
FEATURES             Location/Qualifiers
     mRNA            776..6687
     CDS             926..1834
                     /product="crtE protein"
                     /gene="crtE"
                     /codon_start=1
                     /translation="MTVCAKKHVHPTRSAAEQLLADIDRRLEQLLPVEGERDFVGAAM
                     REGALAPGKRIRPMLLLLTARDLGCAVSHEGLLDIACAVEMVHAASLILDDMPCMDDA
                     QIVGGRPTVHCQYGEHVAILAAVALLSKAFGVIADADGLTALAKNRAVSELSNAIGMQ
                     GLVQGQFKDLSEGDKRRSAEAILMTNHFKTSTLFCASMQMASIVANASSERRDYLHRF
                     SLDLGQAFQLLDDLTDGMADTGKDINQDEGKSTLVNLLGSRAVEERLRHSLHLASEHL
                     SAACQNGHSTQQFIQAWFDKKLAAVS"
     CDS             1844..3139
                     /product="crtX protein"
                     /gene="crtX"
                     /codon_start=1
                     /translation="MSHFAAIAPPFYSHVRALENLAQELVARGHQVTFIQQQDTKPLI
                     QHPHIGFHAVGEESHPSGSLSRLLHLAAHPLGPSMLRLINEMARTSDMLCLALPRAFN
                     DLAIDGVIVDQMEPAGALVAEALGLPFVSVACALPLNREPDLPLAVMPFDYGTSDAAR
                     ERYAASEKIYDWLMRRHDRVIAHHSHRMGLAPRDKLHQCFSPLAQISQLIPELDFPRK
                     ALPACFHAVGPLRETQTTSTSSPLYFSPSEKPRIFASLGTLQGHRYGLFKAIVKACEE
                     IDGQLLLAHCGRLTAFQAEELARSRHTQVVDFADQSAALSQAQLAITHGGMNTVLDAI
                     NYRTPLLALPLAFDQPGVASRIVSHGIGKRASRFTTSHALARHMRALLTNVDYPQRMT
                     KIQTALRLAGGTMAAADIVEQAMRTGQPVLKGSGYATAL"
     CDS             3123..4271
                     /product="dycopene cyclase"
                     /gene="crtY"
                     /codon_start=1
                     /translation="MRPHYDLILVGAGLANGLIALRLQQQQPDMRILLIEAAPQAGGN
                     HTWSFHDADLTESQHRWVAPLVVYHWPDYQVRFPTRRRKLNSGYFSVTSQRFAEVLQQ
                     KFGQHLWISRAVAEVHADAVRLNNGQVISASAVIDGRGYTPNSALNVGFQAFIGQEWR
                     LSKPHGLSSPIIMDATVDQQNGYRFVYSLPLSATELLIEDTHYIDNATLEPERARQNI
                     RDYAAQQDWQLQTLLREEQGALPITLTGDSTAFWQQQPLACSGLRAGLFHPTTGYSLP
                     LAVALADRLSALDVFTSSSIHQAITHFAHERWQQQRFFRMLNRMLFLAGPADSRWRVM
                     QRFYGLPEDLISRFYAGKLTLTDRLRILSGKPPVPVLAALQAIMTTHR"
     CDS             4283..5761
                     /product="phytoene dehydrogenase"
                     /gene="crtI"
                     /codon_start=1
                     /translation="MKPTTVIGAGFGGLALAIRLQAAGIPVLLLEQRDKPGGRAYVYE
                     DQGFTFDAGPTVITDPSAIEELFTLAGKQLKDYVELLPVAPFYRLCWESGKVFNYDND
                     QAQLEAQIQQFNPRDVEGYRQFLDYSRAVFKEGYLKLGTVPFLSFRDMLRAAPQLAKL
                     QAWRTVYSKVASYIEDEHLRQAFSFHSLLVGGNPFATSSIYTLIHALEREWGVWFPRG
                     GTGALVKGMIKLFQDLGGEVVLNAKVSHMETTGDTIEAVHLEDGRRFPTRAVASNADV
                     VHTYRDLLSQHPAAVKQSKKLQTKRMSNSLFVLYFGLNHHHDQLAHHTVCFGPRYREL
                     IHEIFNHDGLADDFSLYLHAPCVTDSSLAPEGCGSYYVLAPVPHLGTANLDWTVEGPR
                     LRDRIFEYLEQHYMPGLRSQLVTQRMFTPFDFRDQLNAYQGSAFSVEPVLTQSAWFRP
                     HNRDKTINNLYLVGAGTHPGAGIPGVIGSAKATAGLMLEDLI"
     CDS             5797..6687
                     /product="prephytoene pyrophosphate synthase"
                     /gene="crtB"
                     /codon_start=1
                     /translation="MAVGSKSFATASKLFDAKTRRSVLMLYSWCRHCDDVIDDQTLGF
                     QTDQPSLQTPEQRLMQLEMKTRQAYAGSQMHEPAFAAFQEVAMAHDIAPAYAFDHLEG
                     FAMDVREAEYIQLDDTLRYCYHVAGVVGLMMAQIMGVRDNATLDRACDLGLAFQLTNI
                     ARDIVEDAHVGRCYLPASWLENEGLNKENYAAPENRQALSRIARRLVQEAEPYYLCAT
                     AGLAGLPLRSAWAIATAKQVYRKIGVKVEQAGTEAWDHRQSTTTPEKLSLLLMASGQA
                     ITSRMRPHPPRPAHLWQRPI"
BASE COUNT     1580 a   1849 c   1858 g   1678 t
ORIGIN
        1 acgctggctg accggggtca ggctttttat ccagtttacg atctaaaaca ggaatgggca
       61 taaaacagtc aaaggattga taaaattgct aagctggtaa gatggctccc aataaccaga
      121 gataacacag tgcaacatta tggattgatg gcgcttttgc tcgtttcctg ttgggcaaac
      181 gcccataaca tcgttgtcgg gcagcctcta ccgtcggtct ttattgcgga taaaggtgag
      241 atgcggctcg atagcggaaa gattgactat caaaaatgga acagtctgac tctgacgggg
      301 aaaacacgtt taattattca cgttgcgggt cgattatcgg caaaagaaca gtcggcagct
      361 ctcattgacg ctcttcagca cgccaacctg cctaaaaaac aattccagac cacgaccatc
      421 gtcaatactg atgatgcttt acctggcagc agtctgtttg tggtcaatag tatccgtcgc
      481 cagtaaaaaa gcatcgccat gggaacagtt tattatcgac agtagcggtg tggcacagca
      541 ccgctggcag cttaagcccg aaggtgcggc agtcattgtg ctggaccaga agggacaggt
      601 gaaatttgct aaagacacgg cgctcagtgc taatgacgtt tctcaggtaa ttgcgacatt
      661 acgtgcactg gcaagctgaa ccccgggcgc tcaggaaaac ctgtcacgcc acaggcaaat
      721 acgacggagg tttatgaatt ctccaccttt tccacaggct caactagtat taattatgtg
      781 gatttagcga gaaaaaacct gaaaccctaa atgtaaaaat aacgggtaag cctgccaacc
      841 atgttatggc agattaagcg tttctttaaa gggcatcgca cttttagcgt tgtcataaat
      901 gtatccgttg ataaggacag cccgaatgac ggtctgcgca aaaaaacacg ttcaccccac
      961 tcgcagtgct gctgagcaat tactggctga tattgatcgg cggcttgagc agttattgcc
     1021 tgtcgaggga gaacgggatt ttgtaggtgc cgcgatgcgt gagggcgcgc tggcaccggg
     1081 taaacgtatt cgcccgatgc tgttgttgct cacagcacgc gatctgggct gcgctgtcag
     1141 ccacgaggga ttgctggata tcgcctgcgc cgtggaaatg gtgcacgcgg catcgttgat
     1201 tcttgatgat atgccctgta tggatgatgc gcagatcgtc ggcggccgcc ctaccgttca
     1261 ctgtcagtac ggcgagcatg tggcaatact ggcggcggtc gccttactga gcaaagcctt
     1321 tggggtcatt gctgatgcag atggcctgac cgcgctggca aaaaaccgcg cagtgtcaga
     1381 attatcaaac gccattggta tgcaaggttt ggtacagggt cagtttaagg atctgtcaga
     1441 aggcgataag cgccgcagcg ctgaagccat tttgatgacg aaccacttta aaaccagcac
     1501 cctgttttgc gcgtctatgc agatggcctc aattgtggcg aatgcctcca gcgaacgacg
     1561 cgattaccta caccgttttt cgcttgatct cggccaagcg tttcaactgc tggacgactt
     1621 aacggatggc atggctgaca cgggtaagga catcaatcag gatgaaggca aatcgaccct
     1681 ggtcaattta ttaggctcca gagcagttga agagcgtctg cggcacagtc tgcatctcgc
     1741 cagtgagcac ctctcagccg cgtgtcaaaa cggtcattct actcaacaat ttatccaggc
     1801 ctggtttgac aaaaaactcg ctgccgtcag ttaaggatgc tgcatgagcc acttcgcggc
     1861 gatcgctccg cctttttaca gccatgtccg tgcactggaa aacctcgctc aggagttagt
     1921 ggctcgcggt catcaggtga cctttattca gcaacaggac actaaaccac tcattcagca
     1981 tccccacatc ggatttcatg ctgtaggaga agaaagccat ccgtctggct cattatcgcg
     2041 cctgctgcac ttagcggccc atcctctggg gccgtcaatg cttcggctca tcaatgaaat
     2101 ggcgcgcacc agcgatatgc tgtgcctggc gctccccaga gcctttaacg atttggcgat
     2161 agatggtgtc atcgttgatc agatggagcc agcaggcgct ctggtcgctg aagcgctggg
     2221 gctgccgttt gtctcggttg cctgtgcact cccgcttaat cgcgaaccgg atttgcccct
     2281 ggcggtgatg ccttttgact atggcaccag cgatgccgca cgtgagcgct atgcagccag
     2341 cgaaaaaatt tatgactggc tgatgcgtcg gcatgaccgc gtcattgccc atcacagtca
     2401 caggatgggc ttagcacccc gggataaact ccatcagtgt ttttcgccat tggcacaaat
     2461 cagtcagctt attccagaac tggattttcc ccgtaaagcg ctaccggctt gttttcatgc
     2521 tgttggcccc ctgcgagaaa cgcagacgac atcaacctct tcgccgcttt atttttcgcc
     2581 gtctgaaaag ccccgaattt tcgcctcgct gggcacgttg cagggtcatc gctacggtct
     2641 gtttaaagcc atcgtgaaag cctgtgaaga gattgatggt cagcttctgt tggcgcactg
     2701 tggccgcctg actgcctttc aggccgaaga gctggcgcga agccgtcaca cgcaggttgt
     2761 ggattttgcc gatcagtcgg ccgcgctgtc gcaggcgcag cttgccatta cccatggcgg
     2821 gatgaatacg gtgctggatg ccattaatta tcgcacgccc cttttagccc ttccgttggc
     2881 atttgatcag cccggcgtgg cctcacgtat tgtttctcat gggataggaa aacgtgcgtc
     2941 ccgttttacc accagccatg cgctggcgcg ccacatgcgt gcattgctga ccaacgtcga
     3001 ctacccgcaa cgcatgacga aaatccagac cgcccttcgt ttagccggcg gcacaatggc
     3061 cgctgcagat atcgttgagc aggccatgcg caccggccag cccgttttga aggggagcgg
     3121 ctatgcgacc gcattatgat ctgattttag tgggcgccgg gctggctaat ggcctcattg
     3181 ccctgcgcct tcagcagcag caacctgaca tgcgtattct gcttatcgaa gcggcccccc
     3241 aggcgggcgg taatcatacc tggtcctttc acgacgctga tttaaccgag agccaacatc
     3301 gctgggtagc accgctggtg gtatatcact ggcccgacta tcaggtccgc tttcccacgc
     3361 ggcgtcgtaa gctgaacagc ggctattttt ctgtgacttc gcagcgtttt gccgaggttt
     3421 tacaacagaa gtttggtcag cacttatgga ttagccgcgc ggtggcagag gttcacgccg
     3481 acgctgttcg actgaataat ggtcaggtca tcagtgccag cgcggtgatt gatgggcgag
     3541 gttatacgcc aaattcagcg ctgaacgtgg gattccaggc gtttatcgga caggaatggc
     3601 gactcagtaa accgcacggt ttatcttcac ccattattat ggatgccacg gtcgatcagc
     3661 aaaatggtta tcgctttgtt tacagcctgc cgctatctgc gacagaactg ttaatcgaag
     3721 atacccacta tatcgataac gcgacactgg aacctgaacg cgcgcgacaa aatattcgcg
     3781 attatgctgc ccagcaggat tggcagcttc agactctgct tcgtgaggaa cagggcgcat
     3841 tgccaataac cttaacgggc gatagcaccg cgttttggca gcaacagcct ttagcctgca
     3901 gtggactgcg tgccggactg tttcacccca caaccggtta ttcactcccc cttgcggttg
     3961 cgctggccga tcgcctgagt gcgcttgatg tctttacatc gtcctcaatt catcaggcga
     4021 ttacccactt tgcccacgag cgctggcagc agcaacgctt tttccgcatg ttgaatcgca
     4081 tgctgttttt agcgggaccc gccgattcac gctggcgtgt tatgcagcgc ttctatggtt
     4141 tacctgaaga tttaatctcc cgtttttatg cgggcaaact cacgctgacc gatcggctac
     4201 gtattctgag cggcaagccg cctgttccgg tattagcggc attgcaggca attatgacga
     4261 ctcatcgtta aagagcgaca acatgaaacc aactacggta attggtgcag gctttggtgg
     4321 cctggcatta gcaattcgtc tgcaggcggc ggggatccct gtcttactgc ttgagcaacg
     4381 cgacaaaccc ggtggccggg cttatgtcta tgaagatcag ggattcactt ttgatgcagg
     4441 gcctaccgtt attaccgatc ccagcgccat tgaagaactc tttaccctgg cgggaaaaca
     4501 gttaaaagat tacgttgaac tgctgccggt tgcgccgttt tatcgcctgt gttgggagtc
     4561 aggaaaggtt ttcaattacg ataacgatca ggcacagctt gaggcgcaga ttcagcagtt
     4621 taatccacgc gatgttgaag gctatcgtca gtttctggac tattcacgtg cggtgtttaa
     4681 agaaggctat ctgaagcttg gcaccgtgcc tttcttgtcg ttcagggata tgcttcgcgc
     4741 cgcgccccaa ctggcgaaac tgcaggcatg gcgaaccgtt tacagtaaag ttgccagcta
     4801 cattgaggat gaacatcttc gtcaggcgtt ttcttttcac tcgctgttag tgggcggcaa
     4861 tcccttcgcc acctcctcca tttatacgct aatacatgca ctcgaacgcg aatggggcgt
     4921 ctggtttcca cgcggtggta ccggggcatt agtgaaaggg atgataaagc tgtttcagga
     4981 tctgggtggc gaagtggtac tgaatgcgaa ggtcagccac atggaaacca cgggggatac
     5041 cattgaagcc gtgcatttag aggacggacg cagattcccg acccgggctg tcgcttccaa
     5101 tgcggatgtg gttcacacct atcgcgacct gttaagtcag catcctgccg ccgtgaagca
     5161 gtcgaaaaaa ctgcaaacta agcgcatgag caactcattg tttgttctct attttggtct
     5221 gaatcaccat cacgatcagc ttgcccacca tacggtttgt ttcggccctc gctaccgtga
     5281 gctgattcat gaaattttca atcatgacgg actcgcagac gatttctcac tttacctgca
     5341 cgcaccttgt gtcacggatt cgtcactggc accggaaggt tgcggcagtt actatgtgtt
     5401 agctcccgtg ccacatttag gcacagcgaa cctcgactgg acggttgaag ggccgcgact
     5461 gcgcgatcgt atttttgagt accttgagca acattacatg cctggcttgc gtagccagtt
     5521 ggttacgcag cgaatgttta cgccgttcga ttttcgcgac cagctcaatg cctatcaagg
     5581 ctcagccttt tctgttgagc ccgttctcac acagagcgcc tggttccgac cgcacaaccg
     5641 cgataaaacc atcaataatc tttacctggt aggtgcaggc acccatcccg gcgcgggcat
     5701 tcccggcgtg atcggctcgg ctaaggcaac tgcaggttta atgctggagg acctgatttg
     5761 aataatccgt cgttactcaa tcatgcggtc gaaacgatgg ctgttggctc gaaaagtttt
     5821 gcgactgcct caaagttatt tgacgcaaaa acccggcgca gtgtacttat gctctactcc
     5881 tggtgccgcc attgtgacga cgtgattgac gatcaaaccc tgggttttca aaccgatcag
     5941 ccgtcattac aaacgcctga gcaacgcctg atgcagttgg agatgaaaac gcgccaggcc
     6001 tatgccggat cgcagatgca cgaaccggcg tttgctgcgt ttcaggaagt ggcgatggcc
     6061 catgatattg cgcccgctta cgcgtttgat catctggaag gctttgccat ggatgtgcgt
     6121 gaggcggagt acattcagtt ggacgataca ttacggtatt gctatcacgt tgcaggcgtg
     6181 gtcggcttga tgatggcgca gattatgggc gtacgcgata acgctacact ggaccgcgcc
     6241 tgtgaccttg ggctggcatt tcagttgacc aacatcgccc gcgatattgt ggaagatgcg
     6301 catgtgggtc gttgttatct gccggcgagt tggctggaaa atgaaggact gaataaagaa
     6361 aattatgcgg caccggaaaa ccgccaggcg ctgagccgca tcgcccgacg tctggtgcag
     6421 gaagccgaac cttactattt gtgcgctacg gcgggactgg ccgggctgcc cttgcgctca
     6481 gcctgggcca tcgccacagc gaagcaggtt tatcgtaaaa ttggggtgaa ggttgaacag
     6541 gcaggaacag aagcctggga ccatcggcag tcaaccacga cacccgaaaa attatcgctt
     6601 ctgttgatgg catccggtca ggccattact tcccggatgc ggcctcatcc gcctcgccct
     6661 gcccatctct ggcagcgccc gatttagccg aatgtcgttc tcttagcgtc gcctgaagtt
     6721 ttgatagcgg cggcgcatag agaaaaccaa aagaaacaca gccttctttg cctcttacgg
     6781 catgatgcat gcggtgggcc atatacaacc gcttcaggta gcctttgcgc ggaatatccg
     6841 gcaacggcca acgctgatgc accagcccgt cgtgaaccat gaaatagagc aatccatagg
     6901 ccgtcatgcc cgcaccaatc cactgaagcg gccacattcc cgtactgccc acataaataa
     6961 gcagg
//