From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Haemophilus somnus 31 kDa antigen gene, complete cds.
Date: 29 Dec 92 21:26:20 GMT
Distribution: bionet
Checksum: 01380 5

LOCUS       HEAANT31KD   1730 bp ds-DNA             BCT       14-DEC-1992
DEFINITION  Haemophilus somnus 31 kDa antigen gene, complete cds.
ACCESSION   L07795
KEYWORDS    31 kDa antigen.
SOURCE      Haemophilus somnus (strain 8025) DNA.
  ORGANISM  Haemophilus somnus
            Prokaryotae; Facultatively anaerobic gram-negative rods;
            Pasteurellaceae.
REFERENCE   1  (bases 1 to 1730)
  AUTHORS   Won,J. and Griffith,R.
  TITLE     Cloning and sequencing of the gene encoding a 31-kilodalton antigen
            of Haemophilus somnus
  JOURNAL   Unpublished (1993)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     CDS             <1..303
                     /pseudo
                     /function="unknown"
                     /product="unknown"
                     /codon_start=1
                     /note="putative"
     terminator      1326..1347
     CDS             487..1308
                     /function="Possess hemolytic activity and
                     immunoreactivity"
                     /evidence="EXPERIMENTAL"
                     /citation=[1]
                     /note="According to deletion mutation data, amino-terminal
                     aminoacids of 31-kDa antigen are important for hemolytic
                     activityand carboxy-terminal amino acids are resoinsible
                     forimmunoreactivity"
                     /product="31 kDa antigen"
                     /codon_start=1
                     /translation="MKLSRFVLSAVVAATLSACGNLSNVTEQGTSDNLKWPKIDQSRF
                     NHDGSQFGSWPNWDNLRMVERGMNKDQLYNLLGRPHFSEGLYGVREWDYAFNYRENGE
                     HKICQYKILFDKNMNAQNFYWFPNGCNGYASFSLNTDLLFDFDQDTLTSKGEEAVDNV
                     AMQLEAFSAKEIKIVGFTDRLGTDSYNLDLSQRRADRVKERLIEKGLNIDIIAIGYGK
                     TQQIKACNDVPAKELKDCLRPNRRVEISAYGNISKKYGNGELKGGTTGPSLYYEK"
BASE COUNT      602 a    246 c    330 g    552 t
ORIGIN      
        1 gatctagaag acactcattt attgtttaaa caattcgtta cacaaaacag accgcacttg
       61 gatattgata aaattgcaac aggtgaacat tggttcggca aacaagcatt agcgttacat
      121 ttagttgatg aaattgcgac aagtgatgat ttattgttac aagcaatcaa agataaaaca
      181 gtgctttcgg taaaatatat gatgaaaaaa tcactcattc aaaaattagg caaacaagca
      241 gaagaaagtg ctaataatgt gttgttacga ttgctgaaac gtgatgagag tactttgata
      301 taagcttact caacattgcc caaaaagaga ttaaaatttg gtttacattt ttgttgtttg
      361 atattaatat ctcaagcagt gctgacgtga tttaatgcga caatattttt gtaaagatat
      421 aaaattaaat cgtaagctat tcaaaaaatt tgaagaaatt tatatacata aaattaaggt
      481 aaaataatga aactatcacg ttttgtatta tctgctgttg tagcagcaac attgtcggct
      541 tgtggtaatt taagtaatgt caccgaacag ggtacatcag ataacttgaa gtggcctaaa
      601 attgatcagt caagatttaa tcatgatggt agccaatttg gatcgtggcc aaactgggat
      661 aacctacgta tggttgaacg cggaatgaat aaagatcaac tgtataacct attgggacgt
      721 ccgcattttt cggaaggttt atatggtgtt cgtgagtggg actatgcttt taattatcgt
      781 gagaatggtg agcataaaat ttgccaatac aaaattttat tcgataaaaa tatgaatgcc
      841 caaaatttct attggtttcc aaatggttgt aacggttatg catcttttag tttaaatacg
      901 gatctcttat ttgattttga tcaagatacg ttgacctcaa aaggtgaaga agctgttgat
      961 aatgttgcaa tgcaattaga agcattttca gccaaagaaa ttaaaattgt tggttttact
     1021 gatcgattgg gtacagattc ttataacttg gatctttctc aacgccgagc tgatcgtgtt
     1081 aaagagcgtt taattgaaaa agggttaaat attgatatta tcgctattgg atatggtaaa
     1141 acgcaacaga ttaaggcttg caatgatgta ccagccaaag aactaaaaga ttgcttacgt
     1201 ccaaaccgtc gtgttgaaat ttcagcatac ggaaacatct caaaaaaata tggtaatggt
     1261 gagctgaagg ggggtacaac tggtccatca ttatattacg aaaaataatc tctcttgata
     1321 taggtaaggg catcaaacga tgccctttat ttatagaatt atttatcact tttgtgcaaa
     1381 atttgattaa tcaatttctg agtgagagaa acataacttt gctttttatt ataatagcga
     1441 tagctgaaat tgagcagata atagagctga taaataagtt ttctttctaa gtaaccttct
     1501 tccagtggat atatttcatt atagcgttga taaaaattag ttgggaaagg ctcaaaaagt
     1561 gatgaaaaag caatatcgca ttctctatct ccccaatagc aagcaggatt gcagacaaaa
     1621 attttatcat ctacttgaat acagttttca atccataaat tgccatgcag tatagagggt
     1681 ttggggttat gtttgctcaa agtatctgca acaatttgca caatgagatc
//