From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: S. thermophilum tryptophanase gene.
Date: 9 Jan 93 05:47:57 GMT
Distribution: bionet
Checksum: 10354 5

LOCUS       STHTNA1      2026 bp ds-DNA             BCT       09-JAN-1993
DEFINITION  S. thermophilum tryptophanase gene.
ACCESSION   D10012
KEYWORDS    tryptophanase.
SOURCE      Symbiobacterium thermophilum (strain IAM 13621) DNA.
  ORGANISM  Symbiobacterium thermophilum
            Prokaryotae; Bacteria.
REFERENCE   1  (bases 1 to 2026)
  AUTHORS   Hirahara,T., Suzuki,S., Horinouchi,S. and Beppu,T.
  TITLE     Cloning, nucleotide sequences, and overexpression in Escherichia
            coli of tandem copies of a tryptophanase gene in an obligately
            symbiotic thermophile, symbiobacterium thermophilum
  JOURNAL   Appl. Environ. Microbiol. 58, 2633-2642 (1992)
  STANDARD  full automatic
COMMENT     These data kindly submitted in computer redable form by: Sueharu
            Horinouchi Department of Agricultural Chemistry Faculty of
            Agriculture The University of Tokyo Bunkyou-ku Tokyo 113 Japan
            Phone:  03-3812-2111 x5147 Fax:    03-3812-0544.
FEATURES             Location/Qualifiers
     RBS             396..402
     CDS             410..1771
                     /EC_number="4.1.99.1"
                     /gene="TNA1"
                     /product="tryptophanase"
                     /codon_start=1
                     /translation="VPKGEPFKIKMVEPIRLIPREDRERALKEAHYNPFFLRSSDVYI
                     DLLTDSGTGAMSQFQWSAMMLGDESYAGASSYYRLKETVTDITGYEYVIPTHQGRGAE
                     KVAFSQLITRPGMYVLSNMFFDTTRGHVQLGGRPVDLLIDVPTEEYHPFKGNMDTERL
                     EQFIREHGAENIACIVMTVTNNSAGGQPVSMANIRETYQIARKYGVLVLFDVARYAEN
                     CHFIRMREEGYADKHPIDIAREMFSYGDGLMMSAKKDALVNIGGLLAFRDEELFTKVG
                     AAVVPFEGFLTYGGLAGRDLEAMAVGLREALDPDYLAYRVGQVQYLGEMLRNAGIPIQ
                     WPVGGHAVFIDAAKFLPHVPWDQFPGHALTLALYLEGGVRTVEVGSLMIGRDPETGEN
                     VRGPFEFTRLAIPRRVYTNLHLEDVAETVINAFQKREEIRGVKFAREPKVLRHFTAWF
                     DPA"
BASE COUNT      371 a    634 c    649 g    372 t
ORIGIN      
        1 gtcgaccggc gtatgggtgt aggtcttctg attgatccgc aggtgccggt tcaacacgtc
       61 aaccaggttc agcctttgcg ctacccaacc aagagcgaca agaaaaccat gccgggtggt
      121 gcatttgatg gcttgcgcag tgacttcggg cgtcgtacca tgagactgca tcagtcgaac
      181 ctccacatgg tggtttaaga ggggcaaatc ccaccatgta tcattggttc agactgatgc
      241 tttttcaatg ggtttccatt gtacggagtg cgggttattc acgcgaaact tgagctagca
      301 gccgtttaca ggacttaaca ggtgatcgga gggctcgcct gacatacggg cgcgacggtt
      361 gctccatgct atgaccgaat cggtccactc tggcaaagga ggagttcacg tgcccaaggg
      421 cgagcccttc aagatcaaga tggtggagcc catccggctg atcccccggg aggaccgtga
      481 gcgggcgctg aaggaagcgc attacaaccc gttcttcctc cgatcttctg acgtctacat
      541 cgacctgctc accgactccg gtacgggcgc gatgagccag ttccagtggt ccgccatgat
      601 gctgggcgac gagtcgtacg ccggcgcaag cagctactac aggctgaaag agacggtcac
      661 cgacatcacg gggtacgagt acgtcatccc gacccaccag ggccgcggcg cagagaaggt
      721 cgccttctcg cagctcatca cccggccggg catgtacgtg ctttccaaca tgttcttcga
      781 cacgacccgc ggccacgtgc agctcggcgg ccggcccgtc gatctgctca tcgacgtacc
      841 caccgaggag taccacccct tcaagggcaa catggacacg gagaggctgg agcagttcat
      901 ccgggagcac ggtgcggaga acatcgcctg catcgtgatg accgtgacca acaactccgc
      961 gggcgggcag cccgtctcca tggccaacat ccgggagact taccagatcg cccggaagta
     1021 cggcgtgctg gtcctgttcg acgtggcccg gtacgccgag aactgccact tcatccggat
     1081 gcgggaggaa ggctacgccg acaagcaccc catcgacatc gcccgggaga tgttctccta
     1141 cggcgacggg ctgatgatga gtgccaagaa ggacgcgctg gtcaacatcg gcgggctcct
     1201 cgcctttcgg gatgaggaac tgttcaccaa ggtcggcgcg gccgtggtgc cgttcgaggg
     1261 cttcctgacc tacggcggcc tggccgggcg cgatctggag gccatggcgg tgggcctccg
     1321 ggaggcgctg gaccccgact acctggcgta ccgggtgggg caggtgcagt acctgggcga
     1381 gatgttgcgg aacgcgggca tcccgatcca gtggccggtg ggcggccatg cggtgttcat
     1441 cgatgccgcg aagttcctgc cgcacgtccc gtgggaccag ttccccggcc atgccctgac
     1501 cctcgcgctc tacctggagg gcggcgtgcg caccgtagag gtcggctcgc tgatgatcgg
     1561 ccgcgacccc gagaccggcg agaacgtgcg aggcccgttc gagttcaccc ggctggcgat
     1621 tccgcggcgg gtctacacca acctgcacct ggaggacgtc gccgagaccg tgatcaacgc
     1681 ctttcagaag cgcgaggaga tccgcggcgt gaagttcgcc cgggagccca aggtgctccg
     1741 ccacttcacg gcctggttcg acccggctta gaccagggct ttcggcaccc gttgacatag
     1801 ggcggccccg gctgtcccgc atgcgtcggg gccgtccact ccacctgttg ccacagagga
     1861 ggccgcatca catggaggca cagcgcgatc agtggaaatc ccgatccgga ttcatcttcg
     1921 ccacgatcgg tgctgccgtg gggctgggga acttctggcg tttcccgttc atggcgtatc
     1981 agaacggcgg cggggcgttc ctgcttccct acttcgtggc cctgct
//