From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: A. shirousamii DNA for glucoamylase, complete cds.
Date: 30 Jul 92 10:00:34 GMT
Distribution: bionet
Checksum: 11818     7

LOCUS       ASNGLA       2614 bp ds-DNA             PLN       30-JUL-1992
DEFINITION  A. shirousamii DNA for glucoamylase, complete cds.
ACCESSION   D10460 D01115
KEYWORDS    glucoamylase; starch inducible.
SOURCE      Aspergillus shirousamii (strain RIB 2504) DNA.
  ORGANISM  Aspergillus shirousamii
            Eukaryota; Fungi; Eumycota; Ascomycotina; Plectomycetes;
            Eurotiales; Trichocomaceae.
REFERENCE   1  (bases 1 to 2614)
  AUTHORS   Shibuya,I., Gomi,K., Iimura,Y., Takahashi,K., Tamura,G. and Hara,S.
  TITLE     Molecular cloning of the glucoamylase gene of Aspergillus
            shirousamii and its expression in Aspergillus oryzae
  JOURNAL   Agric. Biol. Chem. 54, 1905-1914 (1990)
  STANDARD  full staff_review
COMMENT     Submitted (20-Jan-1992) to DDBJ by:
            Ichiro Shibuya
            Institute for Production
            Research and Development
            967 Azamatsuyama
            Kashiwa, Chiba 277
            Japan
            Phone:  0471-72-3900
            Fax:    0471-75-0290
FEATURES             Location/Qualifiers
     mat_peptide     join(337..478,553..836,892..988,1051..1688,1747..2430)
                     /product="glucoamylase"
                     /codon_start=1
     CDS             join(265..478,553..836,892..988,1051..1688,1747..2433)
                     /EC_number="3.2.1.3"
                     /product="glucoamylase"
                     /gene="GLA"
                     /codon_start=1
                     /translation="MSFRSLLALSGLVCSGLASVISKRATLDSWLSNEATVARTAILN
                     NIGADGAWVSGADSGIVVASPSTDNPDYFYTWTRDSGIVLKTLVDLFRNGDTDLLSTI
                     EHYISSQAIIQGVSNPSGDLSSGGLGEPKFNVDETAYAGSWGRPQRDGPALRATAMIG
                     FGQWLLDNGYTSAATEIVWPLVRNDLSYVAQYWNQTGYDLWEEVNGSSFFTIAVQHRA
                     LVEGSAFATAVGSSCSWCDSQAPQILCYLQSFWTGSYILANFDSSRSGKDTNTLLGSI
                     HTFDPEAGCDDSTFQPCSPRALANHKEVVDSFRSIYTLNDGLSDSEAVAVGRYPEDSY
                     YNGNPWFLCTLAAAEQLYDALYQWDKQGSLEITDVSLDFFKALYSGAATGTYSSSSST
                     YSSIVSAVKTFADGFVSIVETHAASNGSLSEQFDKSDGDELSARDLTWSYAALLTANN
                     RRNSVVPPSWGETSASSVPGTCAATSASGTYSSVTVTSWPSIVATGGTTTTATTTGSG
                     GVTSTSKTTTTASKTSTTTSSTSCTTPTAVAVTFDLTATTTYGENIYLVGSISQLGDW
                     ETSDGIALSADKYTSSNPPWYVTVTLPAGESFEYKFIRVESDDSVEWESDPNREYTVP
                     QACGESTATVTDTWR"
     exon            one-of(1747..2542,1747..2543)
     polyA_site      one-of(2542,2543)
     CAAT_signal     76..79
     TATA_signal     147..152
     exon            217..478
                     /number=1
     sig_peptide     265..336
                     /codon_start=1
     intron          479..552
                     /number=1
     exon            553..836
                     /number=2
     intron          837..891
                     /number=2
     exon            892..988
                     /number=3
     intron          989..1050
                     /number=3
     exon            1051..1688
                     /number=4
     intron          1689..1746
                     /number=4
     exon            1747..2542
                     /number=5
                     /note="potential"
     exon            1747..2543
                     /number=5
                     /note="potential"
BASE COUNT      521 a    726 c    716 g    651 t
ORIGIN
        1 gtcgttctga agagctgaag tggcgagatg gtctctgcag gaataccaag ctagatgtta
       61 agcgatgctg cactgcaatc tgtactgtgt ggtgatgcat gtgcttcttc cttcaacttc
      121 ccccctcctg cagatgctgg tttggatata aactgacgtg gtcgctcagg ggtcactgag
      181 ggggtgaaac tagcttcctc cccctcaaga cgcgacaact gactgactga gcttcatcct
      241 caacatcgtt actcccccac cgcaatgtcg ttccgatctc ttctcgccct gagcggcctt
      301 gtctgctcgg ggttggcaag tgtgatttcc aagcgcgcga ccttggattc gtggttgagc
      361 aacgaagcga ccgtggctcg tactgccatc ctgaataaca tcggggcgga cggtgcttgg
      421 gtgtcgggcg cggactctgg cattgtcgtt gccagtccca gcaccgataa cccggactgt
      481 atgttttgag ttcggattat gaatgtgtct tgggtgaatg atggtgactg gcgtgtcttt
      541 tgatgattgt agacttctac acctggactc gcgactctgg tatcgtcctc aagaccctcg
      601 tcgacctctt ccgcaatgga gatactgatc tcctttccac cattgagcac tacatctcct
      661 ctcaggcaat tattcagggt gtcagtaacc cctctggtga tctgtccagc ggtggtcttg
      721 gtgagcccaa gttcaatgtc gatgagactg cctacgccgg ttcttgggga cggccgcagc
      781 gtgatggtcc tgccctgaga gcaactgcta tgatcggctt tgggcagtgg ctgcttgtat
      841 gttctccacc cccttgcgtc tgatctgtga catatgtagc tgactggtca ggacaatggc
      901 tacaccagcg ctgcaacaga gattgtttgg cccctcgtta ggaacgacct gtcgtatgtg
      961 gctcagtact ggaaccagac gggatatggt gtgtttgatt gatcggggtt caagggtgtt
     1021 tgtgcatcgg agctaacttc gcggtcgcag atctctggga agaagttaat ggctcgtcct
     1081 tcttcactat tgccgtgcaa caccgcgccc tcgtcgaagg tagtgccttc gcgacggccg
     1141 tcggctcgtc ctgctcctgg tgtgattcgc aggcacctca gattctctgt tacttgcagt
     1201 ccttctggac cggcagctac atcctggcca actttgacag cagccgttcc ggcaaggaca
     1261 caaacaccct cctgggaagc atccacacct ttgatcctga ggctggatgc gacgactcca
     1321 ccttccagcc ctgctccccg cgtgcgctcg ccaaccataa ggaggttgta gactctttcc
     1381 gctcgatcta tactctcaac gatggtctca gtgacagtga ggcggttgcg gtcggtcggt
     1441 accctgagga tagctactac aacggcaacc cgtggttcct gtgcaccttg gctgccgcgg
     1501 aacagctgta cgatgctctg taccagtggg acaagcaggg gtcgttggag atcacagacg
     1561 tgtcacttga cttcttcaag gctctgtaca gtggtgctgc caccggcacg tactcttcgt
     1621 ccagctcgac ctatagcagc attgtgagtg ccgtcaagac tttcgctgat ggttttgttt
     1681 ctattgtggt aagtctacgc tagacgagcg ctcatattta cagagggtgc gtactaacag
     1741 gattaggaaa ctcacgccgc aagcaacggc tctctgtctg agcaattcga caagtctgat
     1801 ggcgacgagc tttctgctcg cgatctgacc tggtcttacg ctgctctgct gaccgccaac
     1861 aaccgtcgta attctgtcgt gcccccgtct tggggtgaga cctctgccag cagcgtgccc
     1921 ggcacctgtg cggctacctc tgcctctggt acctacagca gtgtgaccgt cacctcgtgg
     1981 ccgagcatcg tggctactgg tggcaccact acgacggcta ccaccactgg atcgggcggc
     2041 gtgacctcga ccagcaagac caccacaact gctagtaaga ccagcaccac tacgtcctcg
     2101 acctcctgca ccacccccac tgccgtagct gtgacctttg atctgacggc gaccaccacc
     2161 tacggcgaga acatctacct ggtcgggtcg atctctcagc tcggtgactg ggagaccagc
     2221 gatggcatag ctctgagcgc tgacaagtac acttccagta acccgccttg gtatgtaact
     2281 gtgactctgc cggctggtga gtcatttgag tacaagttca tccgcgttga gagcgatgac
     2341 tccgtggagt gggagagcga cccgaaccgg gaatacaccg ttcctcaggc gtgcggcgag
     2401 tcgaccgcga cggtgaccga cacctggcgg tagactattc attcctattg atatgaagga
     2461 tgagtatgag ggtaattggt tatataatca tgtatgtagt ggatgtgcat aagagcaacg
     2521 aaatggaagc ctgatcatgt gattctttgt attgcgaccg acggaattga ggatattgcc
     2581 gggaaatcgg acaatgccaa gccattgtct tcca
//