From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Vibrio alginolyticus glutamine synthetase (glnA) and nitrogen
Date: 8 Jan 93 05:52:11 GMT
Distribution: bionet
Checksum: 07334 10

LOCUS       VIBGLNNTR    3747 bp ds-DNA             BCT       07-JAN-1993
DEFINITION  Vibrio alginolyticus glutamine synthetase (glnA) and nitrogen
            regulator 1 (ntrB) genes, complete cds and nitrogen regulator 2
            (ntrC) gene, partial cds.
ACCESSION   L08499
KEYWORDS    DNA binding protein; glutamine synthetase; kinase;
            nitrogen regulator.
SOURCE      Vibrio alginolyticus (library: NCIB 11038) DNA.
  ORGANISM  Vibrio alginolyticus
            Prokaryotae; Facultatively anaerobic gram-negative rods;
            Vibrionaceae.
REFERENCE   1  (sites)
  AUTHORS   Maharaj,R., Rumbak,E., Jones,W.A., Robb,S.M., Robb,F.T. and
            Woods,D.R.
  TITLE     Nucleotide sequence of the Vibrio alginolyticus glnA region
  JOURNAL   Arch. Microbiol. 152, 542-549 (1989)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     protein_bind    13..29
                     /bound_moiety="CAP"
                     /gene="glnA"
     TATA_signal     99..104
                     /gene="glnA"
     promoter        132
                     /function="t1 transcriptional start for the promoter"
                     /standard_name="canonical promoter"
                     /gene="glnA"
                     /evidence="EXPERIMENTAL"
     protein_bind    140..156
                     /bound_moiety="NRI-A"
                     /gene="glnA"
                     /note="putative"
     protein_bind    169..185
                     /bound_moiety="NRI-B"
                     /gene="glnA"
     protein_bind    190..206
                     /bound_moiety="NRI-C"
                     /gene="glnA"
     protein_bind    213..229
                     /bound_moiety="NRI-D"
                     /gene="glnA"
     promoter        269..285
                     /standard_name="glnAp2 (nitrogen regulated promoter)"
                     /gene="glnA"
     misc_signal     385..390
                     /gene="glnA"
                     /note="SD sequence"
     protein_bind    2513..(2529.2590)
                     /bound_moiety="NRI"
                     /gene="ntrB"
     misc_signal     2553..2558
                     /gene="ntrB"
                     /note="SD sequence"
     misc_signal     3656..3661
                     /gene="ntrC"
                     /note="SD sequence"
     CDS             399..1805
                     /function="ammonia utilization"
                     /EC_number="6.3.1.2"
                     /gene="glnA"
                     /note="a.a. 268..272 contains an oxidizable histidine; AMP
                     binding site (aa 397)"
                     /product="glutamine synthetase"
                     /codon_start=1
                     /translation="MSVEKVLSLIQENEVKFVDLRFTDTKGKEQHISIPAHQIDADFF
                     EEGKMFDGSSVAGWKGINESDMVMMPDASSAVLDPFTEDATLNIRCDILEPATMQGYD
                     RDPRSIAKRAEDFMRSTGVADTVLIGPEPEFFLFDDVKFATDMSGSFFKIDDVEAAWN
                     TGSDYEEGNKGHRPGVKGGYFPVAPVDSSQDIRSAMCLVMEEMGLVVEAHHHEATAGQ
                     NEIATRFNTLTTKADEIQIYKYVVHNVAHAFGKTATFMPKPLVGDNGSGMHVHQSLAK
                     DGVNLFAGDKYGGLSEMALYYIGGIIKHARAINAFANPSTNSYKRLVPGFEAPVMLAY
                     SARNRSASIRIPVVPSPKARRIEVRFGDPAANPYLCFASMLMAGLDGIKNKIHPGEAM
                     DKDLYDLPAEESAEIPTVAYSLKDALAELDADREFLTAGGVFSDDFIDSYIELKSQDV
                     ERVNMTTHPVEFELYYSV"
     CDS             2563..3609
                     /function="kinase activity"
                     /gene="ntrB"
                     /note="conserved Gly-rich region (aa. 311..316); conserved
                     Lys (aa. 330)"
                     /product="nitrogen regulator 1"
                     /codon_start=1
                     /translation="MDTSLPSAILNNMVTATLILDDGLAIRYANPAAELLFSQSAKRI
                     VEQSLSQLIQHASLDLALLTQPLQSGQSITDSDVTFVVDGRPLMLEVTVSPITWQKQL
                     MLLVEMRKIDQQRRLSQELNQHAQQQAAKLLVRGLAHEIKNPLGGLRGAAQLLEKMLP
                     DPSLTEYTHIIIEQADRLRALVDRLLGPQKPGKKTQENLHQILEKVRQLVELESQRSI
                     VIERDYDPSLPEILMDADQIEQAMLNIVSNAAQILSHQEHGKITIRTRTVHQANIHGK
                     RCKLAARVEITDNGPGIPPELQDTLFYPMVSGREGGTGLGLSISQNLIDQHNGKIDVE
                     SWPGHTTFTIYLPI"
     CDS             3667..>3747
                     /function="binds DNA"
                     /gene="ntrC"
                     /product="nitrogen regulator 2"
                     /codon_start=1
                     /translation="MSKGYVWVVDDDSSIRWVMEKTSPLPT"
BASE COUNT     1116 a    894 c    804 g    933 t
ORIGIN      
        1 tctagtcccg agcattgtgc gatttagcgt aatgcccatt tgttggtcag aacgaattca
       61 ttaaacgcaa ctttattcca atcgatgcat aattcaaatt gactcttgct caaaaactgg
      121 gctgaataag caatgcgcct gtcacattat ggtgcaataa ttattttccg caccacaata
      181 gtgcaagtaa agatcatttt ggtgcaatca tctgcaccaa cgtagcgcca aaaaaacaca
      241 attgcttgaa attaatggaa tatttttttt ggcacggttt tggctttagt taaaacagca
      301 tcgattaatg aaaagtcaaa ttgattaatc aatgccaggt ttcatgaata attcataaat
      361 cgagctttta ccgcttatta acactggagg ttatcaagat gtcagtagaa aaagttctat
      421 cgctgatcca agaaaacgaa gttaagtttg ttgacctacg cttcactgat acaaaaggta
      481 aagagcagca catttcgatc cctgctcacc aaatcgacgc agacttcttc gaagaaggta
      541 aaatgttcga tggttcatca gttgctggct ggaaaggtat caacgaatca gacatggtaa
      601 tgatgcctga cgcatcttct gctgtgcttg acccattcac ggaagacgca acactaaaca
      661 tccgttgtga catcttagag ccggcaacaa tgcaaggcta cgaccgtgac ccacgttcta
      721 tcgcaaagcg cgctgaagac ttcatgcgct ctactggcgt tgcagatact gtacttatcg
      781 gtcctgagcc agaattcttc ctatttgacg acgtgaaatt cgcgactgac atgtcaggct
      841 ctttcttcaa gatcgatgac gtagaagcag catggaacac aggttctgat tacgaagaag
      901 gtaacaaagg tcaccgtcca ggcgttaaag gtggttactt ccctgtagct ccagtggatt
      961 catctcaaga catccgttct gctatgtgtc tagtaatgga agaaatgggt cttgttgttg
     1021 aagcgcacca ccacgaagca acagcgggtc aaaacgaaat cgcaactcgt ttcaacacgc
     1081 taacaaccaa agctgacgaa atccaaatct acaagtacgt tgtacacaac gttgctcacg
     1141 cgtttggtaa aacggcaaca ttcatgccta aaccacttgt tggtgacaac ggtagcggta
     1201 tgcacgttca ccaatctcta gcgaaagatg gtgtaaacct atttgctggt gacaagtacg
     1261 gcggtctatc tgaaatggcg ctttactaca ttggcggtat catcaaacac gctcgtgcaa
     1321 tcaacgcatt tgctaaccca tcaacaaact cgtacaaacg tcttgtacca ggcttcgaag
     1381 cgccagttat gcttgcttac tctgcacgta accgctctgc ttcaatccgt atccctgtgg
     1441 taccaagccc gaaagcacgt cgtatcgaag ttcgctttgg tgacccagcg gctaacccat
     1501 acctatgctt tgcatcaatg ctaatggctg gtcttgacgg tattaagaac aagatccacc
     1561 caggcgaagc aatggataaa gacctttacg atctaccagc agaagaatca gcagaaatcc
     1621 caacggttgc atactcactg aaagacgcac tagcagagct agatgctgac cgtgaattcc
     1681 taacagcggg tggtgtattc tctgacgact tcatcgactc ttacatcgag ctaaaatctc
     1741 aggacgtaga gcgcgtgaac atgacaactc acccagttga gttcgaactt tactactctg
     1801 tataaggtcg acactgtgta aaatgtaaac acagcaacaa aatattaggc tcgctcgcag
     1861 gccttttttg tattcgtcag tcaagattcg acgtttaaca tgaggggata ataaaaatac
     1921 gcttggactt tttaccttgt aggtcaaaga gctaagccat tgaaacgtga aatcatcgat
     1981 caaaagaagg tgagcaatga aagttcagct tattccatcg ctatgtgctc tcagcggatt
     2041 gttaatcgcc tccatcgcat cagcgcaggt tgcttataca tgggtagata aaaatggcgg
     2101 tggttcattt tagtgatacg ccaaaccaag gtgcaaaagc catgccgctg ccaaaccttg
     2161 aagccaggca cccgctccta aggtagaaag cacggaatca ctcgcccctc agaccaagcc
     2221 atcgaaagag ccacaagaca agccggaaaa gaaaaccgat aaacctctcc cattaaagct
     2281 ctcgatgctg actcctcaac ataacgaaac catccgcagc aaccgaggca tcatcaatat
     2341 tcaactggaa ccaagcttgc atctactagc cctataacgg tgcatttaca tagagcaagt
     2401 gttaagccga ccgtaaaagg ggcaaaatga aatatctggt caaaaaaata gcatcactgc
     2461 ggtgcttttc actttttctt ctgagattta ttcgccatac ttaacgttca attgcaccat
     2521 tattgtgcaa cgcacaatct taaagcaaaa caaggacgta atgtggatac cagtcttcct
     2581 agtgccattc tcaacaacat ggtgacagca acgctgattc ttgatgatgg attggctatc
     2641 cgttatgcca atccggctgc cgagttgctg ttttctcaaa gcgcgaaacg tattgttgaa
     2701 caatcgcttt ctcaattgat tcaacatgcg tcgctggatc tcgcgctgct cactcaacca
     2761 ttgcaaagtg gccaaagcat caccgacagt gacgtcactt tcgttgttga tggccgcccc
     2821 ttaatgctag aggtgacagt tagcccaatc acttggcaaa agcagttgat gcttctggtt
     2881 gagatgcgca aaattgatca gcagcgccga ctctctcaag aactgaacca gcacgcccaa
     2941 caacaagcgg cgaaactgtt agtccgaggg cttgcacatg agatcaaaaa cccgctaggt
     3001 ggattacgtg gtgcagcgca attgttagaa aaaatgctgc cagatccctc tctcaccgag
     3061 tatacgcaca tcattattga gcaagccgac cgtttaagag cgcttgttga tcgattactc
     3121 ggaccacaaa agccaggtaa gaagacacaa gaaaacttgc atcaaattct ggaaaaggtc
     3181 cgccagttgg ttgaactaga atcgcaacgt tcaatcgtga ttgaacgtga ttacgacccg
     3241 agcttgccag aaattttgat ggatgcggac caaatcgaac aggcgatgct taatatcgtc
     3301 agtaatgcag cgcaaattct tagccatcaa gaacacggta aaatcaccat tcgcaccaga
     3361 acggtgcatc aagctaatat tcacggcaaa cgctgtaaac tcgctgcgcg tgtcgaaatc
     3421 acagacaacg gcccaggcat tccaccggaa ttacaagaca cgctgttcta tcccatggtc
     3481 agtggccgcg agggaggaac tggcttaggg ctgtctattt cacaaaattt gatcgaccag
     3541 cacaatggaa aaatagacgt agaaagctgg cctggtcaca caacatttac tatttatttg
     3601 ccgatctgat tgcgctaatg gacttcaagt cgcatcagtt tttcggtttg ctgcaaggat
     3661 tactacatga gtaaaggata tgtttgggtc gttgatgacg acagttccat tcgctgggtg
     3721 atggagaaaa cctctcctct cccaaca
//