From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Escherichia coli pantothenate kinase (coaA) gene, complete cds.
Date: 31 Dec 92 01:44:22 GMT
Distribution: bionet
Checksum: 47886 5

LOCUS       ECOCOAA      1540 bp ds-DNA             BCT       18-DEC-1992
DEFINITION  Escherichia coli pantothenate kinase (coaA) gene, complete cds.
ACCESSION   M90071
KEYWORDS    coaA gene; pantothenate kinase.
SOURCE      Escherichia coli (strain K-12) DNA.
  ORGANISM  Escherichia coli
            Prokaryotae; Gram-negative facultatively anaerobic rods;
            Enterobacteriaceae.
REFERENCE   1  (bases 1 to 1540)
  AUTHORS   Song,W. and Jackowski,S.
  TITLE     coaA and rts are allelic and located at kilobase 3532 on the
            Escherichia coli physical map
  JOURNAL   J. Bacteriol. 174, 1705-1706 (1992)
  STANDARD  full automatic
REFERENCE   2  (bases 1 to 1540)
  AUTHORS   Song,W. and Jackowski,S.
  TITLE     Cloning, sequencing, and expression of the pantothenate kinase
            (coaA) gene of Escherichia coli
  JOURNAL   J. Bacteriol. 174, 6411-6417 (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     CDS             271..1221
                     /EC_number="2.7.1.33"
                     /gene="coaA"
                     /evidence="EXPERIMENTAL"
                     /citation=[2]
                     /note="large protein"
                     /product="pantothenate kinase"
                     /codon_start=1
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHAVEEVRLRK"
     CDS             295..1221
                     /EC_number="2.7.1.33"
                     /gene="coaA"
                     /evidence="EXPERIMENTAL"
                     /note="small protein"
                     /product="pantothenate kinase"
                     /codon_start=1
                     /translation="MTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINEDLSLEEVA
                     EIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKSTTARVLQA
                     LLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFVSDLKSGVP
                     NVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVFVSDFVDFS
                     IYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMTLWKEINWL
                     NLKQNILPTRERASLILTKSANHAVEEVRLRK"
BASE COUNT      416 a    358 c    322 g    444 t
ORIGIN      
        1 atgaagaaac agccgaacgg agggcagatt agcacacttt ttcaacatca ttgtgctcaa
       61 caatgcgctc ctgctaaacc ataattcttt ttatcagatg gaatatctgt cacattgctt
      121 ttcaacgata gcttcctggc agagattttt tcttattatt cctccccatc tggtgttacc
      181 ctcctgccca ttaacccatt caacagaact gtgacgcgcc atggcaaata tcgctttgcc
      241 gatagagcta tgaccgccag aaacatgctt atgagtataa aagagcaaac gttaatgacg
      301 ccttacctac agtttgaccg caaccagtgg gcagctctgc gtgattccgt acctatgacg
      361 ttatcggaag atgagatcgc ccgtctcaaa ggtattaatg aagatctctc gttagaagaa
      421 gttgccgaga tctatttacc tttgtcacgt ttgctgaact tctatataag ctcgaatctg
      481 cgccgtcagg cagttctgga acagtttctt ggtaccaacg ggcaacgcat tccttacatt
      541 atcagtattg ctggcagtgt cgcggtgggg aaaagtacaa ccgcccgtgt attgcaggcg
      601 ctattaagcc gttggccgga acatcgtcgt gttgaactga tcactacaga tggcttcctt
      661 caccctaatc aggttctgaa agaacgtggt ctgatgaaga agaaaggctt cccggaatcg
      721 tatgatatgc atcgcctggt gaagtttgtt tccgatctca aatccggcgt gccaaacgtt
      781 acagcacctg tttactcaca tcttatttat gatgtgatcc cggatggaga taaaacggtt
      841 gttcagcctg atattttaat tcttgaaggg ttaaatgtct tacagagcgg gatggattat
      901 ccacacgatc cacatcatgt atttgtttct gattttgtcg atttttcgat atatgttgat
      961 gcaccggaag acttacttca gacatggtat atcaaccgtt ttctgaaatt ccgcgaaggg
     1021 gcttttaccg acccggattc ctattttcat aactacgcga aattaactaa agaagaagcg
     1081 attaagactg ccatgacatt gtggaaagag atcaactggc tgaacttaaa gcaaaatatt
     1141 ctacctactc gtgagcgcgc cagtttaatc ctgacgaaaa gtgctaatca tgcggtagaa
     1201 gaggtcagac tacgcaaata atttgcaggg gagcgaatac tcccctttct tatttttctg
     1261 cactacgcag ggatatttca ccgcccatcc agggttttat tattccatcc tgctcaagta
     1321 ataaagcccc ctgtttgtct attccgcgtg aaatgccaaa tatttcttta tcaccaatga
     1381 taagtttcac tgggcgatta ataaaattat ccagcttttc ccagcgcgac agataaggtg
     1441 ccaatccttc ttgttcgaag agttccaacg ctgcacgaaa atcacgtatt agcatggccg
     1501 ccaacgtatt acgatcgaga ttgatccccg cttcctgcag
//