From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Bacillus subtilis penicillin binding protein 1A (ponA) gene;
Date: 8 Jan 93 05:46:04 GMT
Distribution: bionet
Checksum: 05620 14

LOCUS       BACHEMEHY    6624 bp ds-DNA             BCT       07-JAN-1993
DEFINITION  Bacillus subtilis penicillin binding protein 1A (ponA) gene;
            uroporphyrinogen decarboxylase (hemE) gene; ferrochelatase (hemH)
            gene complete cds, (hemY) gene, complete cds; ORFA, complete cds;
            ORFB 5' end.
ACCESSION   M97208
KEYWORDS    ferrochelatase; hemE gene; hemEHY operon; hemH gene; hemY gene;
            penicillin binding protein 1A; ponA gene;
            uroporphyrinogen decarboxylase.
SOURCE      Bacillus subtilis (strain 168) DNA.
  ORGANISM  Bacillus subtilis
            Prokaryotae; Firmicutes; Endospore-forming rods and cocci;
            Bacillaceae.
REFERENCE   1  (bases 1 to 6624)
  AUTHORS   Hansson,M. and Hederstedt,L.
  TITLE     Cloning and characterization of the Bacillus subtilis hemEHY gene
            cluster which encodes protoheme IX biosynthetic enzymes
  JOURNAL   J. Bacteriol. 174, 8081-8093 (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     CDS             <2..1783
                     /function="cell wall synthesis"
                     /gene="ponA"
                     /citation=[1]
                     /product="penicillin binding protein 1A"
                     /codon_start=1
                     /translation="ITQQLAKNIFLTHDKTFLRKTKEVIIAINLERDYSKDKLLEMYL
                     NQLYFGHGVYGIQAASHYYFNKEVKDLTVSEGAVLAAIPKAPSTYSPILHPDKNKERR
                     DTILGMMNDQGYISAKEAVTAQGRTLGLHVKKQSETPWFDSYIDLVIKEAEDKYSISG
                     EQLLQGGYTIKVPLDSKLQKTAYQVMKEGSYYPGTDQNAEGSAVFINNKTGGVEAAIG
                     GRDYTSKGYNRVTAVRQPGSTFKPLAVYGPAMQEKKFKPYSLLKDELQSYGDYTPKNY
                     DSRYEGEVTMSDAITYSKNAPAVWTLNEIGVETGKSYLKANGIDIPDEGLALALGGLE
                     KGVSPLQLAGAFHTFAANGTYTEPFFISSIIDEDGETIADHKEEGKRVFSKQTSWNMT
                     RMLQQVVKKGTATSGTYHGDLAGKTGSTSYTGVSGATKDAWFAGYTPKITGAVWMGYD
                     KTDQNHYLKAGSSYPTRLFKDILTQAGETGHVFTKPKNVKELESPIELKPVKTLTADY
                     TFKAAGLFTIELKWDAQEDDRAVYRIYVNKDGEETLLDSVEGKGSYEIPYANLFSGAS
                     YKIVPYNTQTKREGEGTDYVQPKLFSS"
     CDS             1905..2966
                     /EC_number="4.1.1.37"
                     /gene="hemE"
                     /evidence="EXPERIMENTAL"
                     /citation=[1]
                     /product="uroporphyrinogen decarboxylase"
                     /codon_start=1
                     /translation="MSKRETFNETFLKAARGEKADHTPVWYMRQAGRSQPEYRKLKEK
                     YGLFEITHQPELCAYVTRLPVEQYGVDAAILYKDIMTPLPSIGVDVEIKNGIGPVIDQ
                     PIRSLADIEKLGQIDPEQDVPYVLETIKLLVNEQLNVPLIGFSGAPFTLASYMIEGGP
                     SKNYNKTKAFMYSMPDAWNLLMSKLADMIIVYVKAQIEAGAKAIQIFDSWVGALNQAD
                     YRTYIKPVMNRIFSELAKENVPLIMFGVGASHLAGDWHDLPLDVVGLDWRLGIDEARS
                     KGITKTVQGNLDPSILLAPWEVIEQKTKEILDQGMESDGFIFNLGHGVFPDVSPEVLK
                     KLTAFVHEYSQNKKMGQYS"
     CDS             3038..3970
                     /function="iron is inserted into protoporphyrin IX giving
                     protoheme IX"
                     /EC_number="4.99.1.1"
                     /gene="hemH"
                     /evidence="EXPERIMENTAL"
                     /citation=[1]
                     /product="ferrochelatase"
                     /codon_start=1
                     /translation="MSRKKMGLLVMAYGTPYKEEDIERYYTHIRRGRKPEPEMLQDLK
                     DRYEAIGGISPLAQITEQQAHNLEQHLNEIQDEITFKAYIGLKHIEPFIEDAVAEMHK
                     DGITEAVSIVLAPHFSTFSVQSYNKRAKEEAEKLGGLTITSVESWYDEPKFVTYWVDR
                     VKETYASMPEDERENAMLIVSAHSLPEKIKEFGDPYPDQLHESAKLIAEGAGVSEYAV
                     GWQSEGNTPDPWLGPDVQDLTRDLFEQKGYQAFVYVPVGFVADHLEVLYDNDYECKVV
                     TDDIGASYYRPEMPNAKPEFIDALATVVLKKLGR"
     CDS             3985..5397
                     /function="involved in a late step of protoheme IX
                     synthesis"
                     /gene="hemY"
                     /evidence="EXPERIMENTAL"
                     /citation=[1]
                     /codon_start=1
                     /translation="MSDGKKHVVIIGGGITGLAAAFYMEKEIKEKNLPLELTLVEASP
                     RVGGKIQTVKKDGYIIERGPDSFLERKKSAPQLVKDLGLEHLLVNNATGQSYVLVNRT
                     LHPMPKGAVMGIPTKIAPFVSTGLFSLSGKARAAMDFILPASKTKDDQSLGEFFRRRV
                     GDEVVENLIEPLLSGIYAGDIDKLSLMSTFPQFYQTEQKHRSLILGMKKTRPQGSGQQ
                     LTAKKQGQFQTLSTGLQTLVEEIEKQLKLTKVYKGTKVTKLSHSGSCYSLELDNGVTL
                     DADSVIVTAPHKAAAGMLSELPAISHLKNMHSTSVANVALGFPEGSVQMEHEGTGFVI
                     SRNSDFAITACTWTNKKWPHAAPEGKTLLRAYVGKAGDESIVDLSDNDIINIVLEDLK
                     KVMNINGEPEMTCVTRWHESMPQYHVGHKQRIKELREALASAYPGVYMTGASFEGVGI
                     PDCIDQGKAAVSDALTYLFS"
     CDS             5543..6118
                     /evidence="EXPERIMENTAL"
                     /citation=[1]
                     /note="ORFA"
                     /codon_start=1
                     /translation="MSIDRKKLILEAATKSFTQFGYKATTMDLVAKLANVGKGTIYTF
                     FKNKEELFDEIFTTLLKEMKQKADEAMEPSLPFHENVHRALFAILEFRKTHQLTIKIF
                     QENAEIGTMAVQEVIQKMERSILSYIKSKIEDGIKSGAIKPCDPELTAFVMLKLYIAL
                     IFDWEKQHPPLDKETIAGLLELYVVKGLSAN"
     CDS             6189..6623
                     /evidence="EXPERIMENTAL"
                     /citation=[1]
                     /partial
                     /note="ORFB"
                     /codon_start=1
                     /translation="MNTIRSQWKDIVTSKKLLIPIIAILFVPLIYSGVFLKAYWDPYG
                     TVDQLPVVVVNQDKGATYEGEKLQIGDDLVKELKDNNNFDWHFSNDLDQSLKDLLNQK
                     YYLVVEIPEDFSKNASTVLDKNPKKLDLKYHTNAGSNYVGATI"
BASE COUNT     2019 a   1381 c   1645 g   1579 t
ORIGIN      
        1 gatcacccag cagcttgcca aaaatatttt tctgacacat gacaaaacat ttctaagaaa
       61 aacaaaagaa gtgatcattg cgattaatct tgaacgggat tacagcaagg acaagctgct
      121 tgaaatgtat ttaaaccagc tttacttcgg acacggggtt tacggcatac aagcggcgtc
      181 ccactattat tttaataaag aagtaaaaga tttgactgta tctgaaggcg ctgttttggc
      241 cgctattccg aaggcgcctt ctacgtactc tccaattttg caccctgata aaaataagga
      301 gcggcgtgat acaattctag gtatgatgaa tgaccaaggc tatatcagtg cgaaagaggc
      361 tgtcaccgca cagggccgga cgcttggact gcatgtgaag aaacagtcgg aaacaccttg
      421 gtttgacagc tacattgacc ttgtgatcaa agaggcggaa gacaagtatt ccatttccgg
      481 agagcagctt cttcaaggcg gatacacgat caaggtgccg ctcgattcga agctgcagaa
      541 gacggcttac caggtgatga aagagggaag ctattatccc ggaacagatc agaatgccga
      601 gggaagcgcg gtctttatta ataacaagac aggcggtgta gaagcggcca tcggcggaag
      661 ggattacaca tccaaaggat ataaccgtgt cacagctgtc cgccagccgg gttccacatt
      721 taagccgctt gctgtatacg gaccggcgat gcaggaaaag aaatttaagc catattctct
      781 attgaaggat gagttgcagt cctacggaga ttatacgccg aaaaactacg acagccggta
      841 tgagggcgaa gtcactatgt ctgacgccat tacgtacagc aaaaacgcgc cggctgtctg
      901 gacgctgaat gagatcggcg tcgaaacagg gaaatcttat ttgaaagcaa acggcattga
      961 tatccctgat gaaggccttg ctcttgctct aggcggcttg gaaaaaggcg tttcaccgct
     1021 ccagcttgcc ggcgcgtttc atacatttgc cgcgaatgga acgtatactg aacccttctt
     1081 tatctcgagc ataatagatg aagacggaga aacaatagcc gatcataaag aagaaggaaa
     1141 gcgggtattc agcaagcaga cgtcttggaa tatgacgaga atgctgcagc aggtagtcaa
     1201 aaagggaacg gccacgtccg gcacctatca cggcgacctg gcgggaaaaa caggttcaac
     1261 atcttataca ggcgtctccg gcgcgacaaa ggacgcctgg tttgcaggct atacgccgaa
     1321 aataaccggg gcagtctgga tgggctatga caaaacggat caaaaccatt atttgaaagc
     1381 cgggagctca tacccgacgc ggctgtttaa ggatattttg acacaggcag gagaaaccgg
     1441 tcatgtgttc acaaagccta aaaacgttaa agaactggaa agcccgattg agctgaagcc
     1501 ggtcaaaaca ctcacagccg attacacatt taaagcagcg gggctcttta ctattgaatt
     1561 aaagtgggac gctcaagagg atgatcgggc cgtatacaga atttatgtaa acaaggacgg
     1621 cgaggagacg cttcttgatt cggttgaagg gaagggaagc tatgaaattc cttatgccaa
     1681 cttattttcg ggcgcgtctt ataaaattgt gccttataat acacagacaa aaagagaagg
     1741 ggaaggaacc gattacgttc agccaaaatt gttttcctct taatggaatt cggcgatttt
     1801 ttgaactttg agctacattt ctataccatt tgcctaaaac aaggtatagt gtaataagtc
     1861 aagaaatacg aacgtatggt tgccgtatga aaggtggaaa tcagatgagt aaacgagaaa
     1921 cgtttaacga gacgttttta aaagctgcgc ggggagaaaa agcggatcac acgcctgtgt
     1981 ggtatatgag gcaagcaggg cgctcacagc cggaataccg caagctgaag gaaaagtacg
     2041 gattgtttga gatcacacat cagcccgaac tttgtgcgta tgtcacaaga ctgccggttg
     2101 agcaatacgg agtcgatgct gcaatccttt ataaagatat catgacgccg ctgccgtcaa
     2161 tcggtgtgga tgttgaaatc aaaaacggga tcggtcctgt gattgatcag ccaatccggt
     2221 ctctggcgga cattgaaaaa ctcggccaga ttgatccgga acaggacgtg ccgtacgtgc
     2281 ttgagacgat taaactgctt gtcaatgagc agctgaacgt cccgctcatc ggtttctcag
     2341 gtgcgccttt tacgcttgca agctatatga tcgaaggcgg cccgtcgaaa aactacaata
     2401 aaacaaaagc cttcatgtac agcatgccag atgcatggaa tctgctgatg tccaagcttg
     2461 ccgacatgat catcgtgtac gtgaaagcgc agattgaggc aggcgcaaaa gcgattcaaa
     2521 tctttgattc gtgggtcggc gcattgaatc aggcagatta cagaacatac atcaaacccg
     2581 tgatgaaccg gatcttttca gagctcgcaa aggagaatgt gccgctgatc atgtttggcg
     2641 ttggcgcaag ccatcttgca ggtgattggc atgaccttcc tctcgatgtt gtcgggcttg
     2701 actggagact cggcattgat gaagccagat caaaaggaat tactaaaaca gtgcagggca
     2761 acctggaccc gtccattttg cttgcgccat gggaagtcat tgagcagaaa acgaaggaaa
     2821 tacttgatca gggtatggag tcagacggct tcattttcaa tcttggccat ggggtatttc
     2881 ctgatgtcag tcccgaggtt ttgaaaaaac tgacagcatt tgtccatgaa tattcacaaa
     2941 acaaaaaaat gggtcaatat tcctaagcat atcttttggt atcttaatga tatcctgtca
     3001 aaatgtttgt atcagatcat taaagagggt gtaaacagtg agtagaaaga aaatggggct
     3061 tctcgtgatg gcgtacggca cgccttataa ggaagaagat attgaacgtt attatacaca
     3121 tatcagaaga ggcagaaagc ctgaacctga aatgcttcaa gatttgaaag acagatacga
     3181 agcgattggc ggcatttcac cgcttgccca aatcacagaa cagcaggcgc ataatctgga
     3241 acagcacttg aatgaaattc aggatgagat tacgtttaaa gcgtatatcg gactgaagca
     3301 tatcgagccg tttattgaag acgccgtagc agagatgcat aaggatggca ttacagaagc
     3361 agtcagcatc gtgctggcgc cgcatttctc cacgttcagc gttcagtcct acaacaaacg
     3421 cgccaaagaa gaggctgaaa aactcggcgg cttaacgatt acgtcagttg aaagctggta
     3481 cgatgaaccg aagttcgtga catattgggt tgaccgagtg aaggaaacat atgcgtcaat
     3541 gcctgaggat gaaagagaaa acgcaatgct catcgtatcc gcacacagcc tgccggaaaa
     3601 aatcaaagaa tttggagacc cgtacccgga ccagcttcat gaatcggcga aattaattgc
     3661 tgaaggcgca ggcgtttccg aatatgctgt cggctggcaa agtgaaggga acacgcctga
     3721 tccttggctc ggacccgatg ttcaggattt aacacgtgat ttattcgaac aaaaaggcta
     3781 tcaagcattt gtatatgttc ctgttgggtt tgtcgcggat cacttagaag tgctttatga
     3841 taatgattat gaatgcaaag tggttactga cgatatcggc gcaagctatt accggccgga
     3901 aatgccaaat gccaagcctg aatttattga tgctttggca acagtcgtat taaaaaaatt
     3961 aggacgttaa agaaggcgat gaacatgagt gacggcaaaa aacatgtagt catcatcggc
     4021 ggcggcatta ccggtttagc cgccgccttc tatatggaaa aagaaatcaa agaaaagaat
     4081 ctgccgcttg agctgacact tgttgaggca agtccgagag tcggcgggaa aatccagact
     4141 gtcaagaaag acggctatat catcgaaaga gggccagact catttctgga acgaaagaaa
     4201 agcgccccgc agcttgttaa agacttaggt cttgagcatt tgcttgtcaa caatgcgacc
     4261 gggcaatcct atgtgcttgt aaaccgcacc ctgcatccaa tgccgaaggg cgctgtaatg
     4321 gggataccga caaaaattgc gccgtttgtt tctacgggtc tgttttcctt gtccgggaag
     4381 gcgagagctg ctatggattt catcctgcct gcaagcaaaa caaaggatga tcagtcattg
     4441 ggagaattct tccgcagacg tgtcggagat gaagtggtcg agaacttaat cgagccgcta
     4501 ctatcaggga tctacgcagg cgacattgac aagctcagcc tgatgtcgac atttccgcaa
     4561 ttttatcaga cggaacaaaa gcatagaagc ctgattctcg gcatgaaaaa aacaaggcct
     4621 caaggctcag gccagcagct gacggcaaaa aaacaagggc agttccaaac tctgtcaacc
     4681 ggtttgcaga cccttgtaga agagatcgaa aagcagttaa agctgacgaa ggtgtataaa
     4741 ggcacaaaag tgaccaaact tagccatagc ggctcttgct attcgctcga actggataac
     4801 ggcgtcacac ttgatgctga ttcagtaatt gtgactgctc cgcataaagc ggctgcggga
     4861 atgctttctg agcttcctgc catttctcat ttgaaaaata tgcactccac atccgtggca
     4921 aacgtcgctt taggtttccc tgaaggctcc gtccaaatgg agcatgaggg cacgggtttt
     4981 gtcatttcaa gaaacagtga ctttgcgatc acagcctgta cgtggacgaa taaaaaatgg
     5041 ccgcacgcag cgccggaagg caaaacgctg cttcgggcat atgtcggaaa agccggagac
     5101 gaatccattg tcgatctatc agataatgac attatcaaca ttgtgttaga agacttaaag
     5161 aaagtcatga acataaacgg cgagccggaa atgacatgtg taacccgatg gcatgaaagc
     5221 atgccgcagt accatgtcgg ccataagcag cgtatcaagg agctgcgtga agcacttgca
     5281 tctgcgtatc cgggtgttta tatgacaggc gcttctttcg aaggtgtcgg cattcccgac
     5341 tgcattgatc aaggaaaagc tgccgtgtct gacgcgctta cctatttatt cagctaaaac
     5401 ctccgcttta tcgcggaggt ttttttgatg tgcaatggca atattgtgta aaaaatgatt
     5461 gaattgaaca gcaggttatg atacatttgc atatgtactt aatgactaaa atgttcaaac
     5521 ggtcagtttc gaggtgaata caatgtcaat agatcgaaaa aagctcattt tggaggcggc
     5581 tacaaagtct ttcacgcagt tcgggtataa agcgacgaca atggatcttg tcgcaaaact
     5641 tgcgaacgtg gggaagggaa ccatttatac ttttttcaaa aacaaagaag agctgtttga
     5701 cgagattttc actactttat taaaggaaat gaagcaaaaa gcggatgaag cgatggagcc
     5761 gagccttccg ttccatgaaa atgtgcacag agcgctgttt gccattctgg agttcaggaa
     5821 aacacatcag ctgacgatta aaattttcca agagaatgcc gagattggta caatggcagt
     5881 tcaggaagtg atccagaaaa tggagcgatc catcctgtct tatatcaaaa gcaagattga
     5941 agacgggatc aaaagcggcg caatcaagcc gtgcgaccct gagctgacag cctttgtgat
     6001 gctgaagctt tatatcgcgc tcatttttga ctgggaaaaa caacaccccc cgcttgataa
     6061 agagacaatt gcgggactgc ttgaattgta tgttgtcaaa ggattgtcag cgaactagat
     6121 aatcctttct ctttgcaaga aaatgaccat tttttatgat gagtcagttt tagaatggag
     6181 gcatcaggat gaacacaata cgaagtcagt ggaaagacat cgtaacaagt aaaaaattat
     6241 tgattcccat catcgccatt ttgtttgtcc cgcttattta cagcggcgta ttcttaaagg
     6301 cttattggga cccgtacggc acagttgacc agctgcctgt cgttgtcgtc aaccaagaca
     6361 agggagcaac gtatgaagga gaaaaacttc aaatcggtga tgatctggtc aaagaattaa
     6421 aggataataa caactttgat tggcattttt ccaatgatct cgaccaatcg ttaaaggact
     6481 tattgaatca aaaatattat ttagttgtcg agattcctga ggatttttct aagaatgcca
     6541 gcactgtatt agacaaaaat ccgaaaaaat tggatctgaa ataccataca aatgcaggtt
     6601 ctaactatgt aggggcaacg atcg
//