From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: E. coli proline/betaine transporter (proP) gene, complete cds.
Date: 29 Dec 92 00:50:16 GMT
Distribution: bionet
Checksum: 22098 5

LOCUS       ECOPROBETT   2138 bp ds-DNA             BCT       04-DEC-1992
DEFINITION  E. coli proline/betaine transporter (proP) gene, complete cds.
ACCESSION   M83089
KEYWORDS    osmoregulator; proline/betaine transporter.
SOURCE      Escherichia coli (strain K-12) DNA.
  ORGANISM  Escherichia coli
            Prokaryotae; Gram-negative facultatively anaerobic rods;
            Enterobacteriaceae.
REFERENCE   1  (bases 1 to 2138)
  AUTHORS   Culham,D.E., Lasby,B., Marangoni,A.G., Milner,J.L., Steer,B.A., van
            Nues,R.W. and Wood,J.M.
  TITLE     Isolation and Sequencing of Escherichia coli Gene proP Reveals
            Unusual Structural Features of the Osmoregulatory Proline/Betaine
            Transporter, ProP
  JOURNAL   J. Mol. Biol. (1992) In press
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     CDS             433..1935
                     /function="active uptake of proline or betaine"
                     /standard_name="ProP"
                     /gene="proP"
                     /evidence="EXPERIMENTAL"
                     /note="Evidence that this open reading frame encodes a
                     transporterincludes deletion/complementation analysis and
                     comparisonwith the sequences of bacterial citrate and
                     alphaketoglutarate transporters"
                     /product="a proline/betaine transporter"
                     /codon_start=1
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVVGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDDIDHEIADLQAKRTRLVQQHPRIDE"
BASE COUNT      466 a    484 c    578 g    610 t
ORIGIN      
        1 tgatttttta agttggctgg gaaagtggac cacggcagat aaacaacccg tttgctggtc
       61 agtgacccaa cgctggcaaa ctgtcgcgct ggggatgcca cgactctgtt cagcgcagcg
      121 tctggcgggg gcaatgctcg aggaaatctt ctctgtaaat ttggcgtaaa taatcagtta
      181 catcaatgag tcctaaacga aatccatgtg tgaagttgat cacaaattta aacactggta
      241 gggtaaaaag gtcattaact gcccaattca ggcgtcaact ggtttgattg tacattcctt
      301 aaccggaggg tgtaagcaaa cccgctacgc ttgttacaga gattgcatcc tgcaattccc
      361 gctccccttt tgcggccgtc gcgctgattt ttctggcgtt tgcggaaatg ggccaactct
      421 gcgaggaaag ctatgctgaa aaggaaaaaa gtaaaaccga ttacccttcg tgatgtcacc
      481 attattgatg acggtaaact gcgtaaagcc attaccgcag catcactggg taatgcaatg
      541 gaatggttcg attttggtgt ttatggtttt gttgcttacg cattaggtaa agtttttttc
      601 ccgggggctg accccagcgt gcagatggtt gctgcacttg ccactttctc cgttcccttt
      661 ctgattcgac cgcttggcgg actcttcttt ggtatgttgg gcgataaata tggtcgccag
      721 aagatcctcg ctatcactat tgtgattatg tcgatcagta cgttctgtat tggcttaata
      781 ccgtcctacg acacgattgg tatttgggca ccgattctgc tgttgatctg taagatggca
      841 caaggtttct cggtcggcgg tgaatatacc ggggcgtcga tatttgttgc ggaatactcc
      901 cctgaccgta aacgtggctt tatgggcagc tggctggact tcggttctat tgccgggttt
      961 gtgctgggtg cgggcgtggt ggtgttaatt tcgaccattg tcggcgaagc gaacttcctc
     1021 gattggggct ggcgtattcc gttctttatc gctctgccgt tagggattat cgggctttac
     1081 ctgcgccatg cgctggaaga gactccggcg ttccagcagc atgtcgataa actggaacag
     1141 ggcgaccgtg aaggtttgca ggatggcccg aaagtctcgt ttaaagagat tgccactaaa
     1201 tactggcgca gcctgttgac atgtattggt ctggtaattg ccaccaacgt gacttactac
     1261 atgttgctga cctatatgcc gagttatttg tcgcataacc tgcattactc cgaagaccac
     1321 ggggtgctga ttattatcgc cattatgatc ggtatgctgt ttgtccagcc ggtgatgggc
     1381 ttgctgagtg accgttttgg ccgtcgtccg tttgtgctac ttggtagtgt tgccctgttt
     1441 gtgttggcga tcccggcgtt tattctgatt aacagtaacg tcatcggcct gatttttgcc
     1501 gggttactga tgctggcggt gatccttaac tgctttacgg gcgttatggc ttctaccttg
     1561 ccagcgatgt tcccgacgca tatccgttac agcgcgctgg cggcggcatt taatatttcg
     1621 gtgctggttg ccggtctgac gccaacgctg gcggcctggc tggtcgaaag ctcgcagaat
     1681 ctgatgatgc ctgcctatta cctgatggta gtggcggtgg ttggtttaat caccggcgta
     1741 accatgaaag agacggcaaa tcgtccgttg aaaggtgcga caccggcggc gtcagatata
     1801 caggaagcga aggaaattct tgtcgagcat tacgataata tcgagcagaa aatcgatgat
     1861 attgaccacg agattgccga tttgcaggcg aaacgtaccc gcctggtgca gcaacatccg
     1921 cgaattgatg aataagctga aacggatggc ctgatgtgac gctgtcttat caggccaatt
     1981 gaactcttaa ggttcactta atctctgacg cgcatactct cctccaggtt aacggaggag
     2041 agtgcaatga aaaaccgtgt ttatgaaagt ttaactaccg tgttcagcgt gctggtggtc
     2101 agcagctttc tttatatctg gtttgccacg tactgatc
//