From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: A.flavus UOX gene for urate oxydase
Date: 25 Jul 92 10:02:17 GMT
Distribution: bionet
Checksum: 24910     4

LOCUS       ASNUOXGENE   1440 bp ds-DNA             PLN       25-JUL-1992
DEFINITION  A.flavus UOX gene for urate oxydase
ACCESSION   X61765
KEYWORDS    urate oxidase.
SOURCE      Aspergillus flavus DNA.
  ORGANISM  Aspergillus flavus
            Eukaryota; Fungi; Eumycota; Ascomycotina; Plectomycetes;
            Eurotiales; Trichocomaceae.
REFERENCE   1  (bases 1 to 1440)
  AUTHORS   Legoux,R., Dumont,X., Delpech,B., Shire,D., Guillemot,J.C.,
            Ramond,P., Caput,D., Ferrara,P. and Loison,G.
  TITLE     Cloning and expression in E. coli of the gene encoding urate
            oxydase of A. flavus
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
COMMENT     *source: strain=ATCC 20047;
            From EMBL    entry AFUOXGENE;  dated 26-JUN-1992.
FEATURES             Location/Qualifiers
     CDS             join(169..305,367..1013,1078..1202)
                     /EC_number="1.7.3.3"
                     /product="urateoxidase"
                     /gene="UOX"
                     /codon_start=1
                     /translation="MSAVKAARYGKDNVRVYKVHKDEKTGVQTVYEMTVCVLLEGEIE
                     TSYTKADNSVIVATDSIKNTIYITAKQNPVTPPELFGSILGTHFIEKYNHIHAAHVNI
                     VCHRWTRMDIDGKPHPHSFIRDSEEKRNVQVDVVEGKGIDIKSSLSGLTVLKSTNSQF
                     WGFLRDEYTTLKETWDRILSTDVDATWQWKNFSGLQEVRSHVPKFDATWATAREVTLK
                     TFAEDNSASVQATMYKMAEQILARQQLIETVEYSLPNKHYFEIDLSWHKGLQNTGKNA
                     EVFAPQSDPNGLIKCTVGRSSLKSKL"
     exon            61..305
                     /number=1
     intron          306..366
                     /number=1
     exon            367..1013
                     /number=2
     intron          1014..1077
                     /number=2
     exon            1078..1295
                     /number=3
BASE COUNT      360 a    413 c    335 g    332 t
ORIGIN
        1 ggatccaaag gatttaaaag ctggcgacaa ttgtcctgtg acttcctctt gtcttgttcc
       61 aaaccctcac tgcctctctc attccttccg gtgcccccga tcctcaatcc aacttgtaca
      121 tacttctccc aactctctgc tatatccttc atattcccat actacaagat gtccgcagta
      181 aaagcagccc gctacggcaa ggacaatgtc cgcgtctaca aggttcacaa ggacgagaag
      241 accggtgtcc agacggtgta cgagatgacc gtctgtgtgc ttctggaggg tgagattgag
      301 acctcgtaag tcaccccccg tctaaaccga tcccttgaaa gtttggctaa taaccgagga
      361 ctgcagttac accaaggccg acaacagcgt cattgtcgca accgactcca ttaagaacac
      421 catttacatc accgccaagc agaaccccgt tactcctccc gagctgttcg gctccatcct
      481 gggcacacac ttcattgaga agtacaacca catccatgcc gctcacgtca acattgtctg
      541 ccaccgctgg acccggatgg acattgacgg caagccacac cctcactcct tcatccgcga
      601 cagcgaggag aagcggaatg tgcaggtgga cgtggtcgag ggcaagggca tcgatatcaa
      661 gtcgtctctg tccggcctga ccgtgctgaa gagcaccaac tcgcagttct ggggcttcct
      721 gcgtgacgag tacaccacac ttaaggagac ctgggaccgt atcctgagca ccgacgtcga
      781 tgccacttgg cagtggaaga atttcagtgg actccaggag gtccgctcgc acgtgcctaa
      841 gttcgatgct acctgggcca ctgctcgcga ggtcactctg aagacttttg ctgaagataa
      901 cagtgccagc gtgcaggcca ctatgtacaa gatggcagag caaatcctgg cgcgccagca
      961 gctgatcgag actgtcgagt actcgttgcc taacaagcac tatttcgaaa tcggtaggtt
     1021 tttctgcatt cccggtatcc cttgttttga agctgtcgct aatgagataa tctttagacc
     1081 tgagctggca caagggcctc caaaacaccg gcaagaacgc cgaggtcttc gctcctcagt
     1141 cggaccccaa cggtctgatc aagtgtaccg tcggccggtc ctctctgaag tctaaattgt
     1201 aaaccaacat gattctcacg ttccggagtt tccaaggcaa actgtatata gtctgggata
     1261 gggtatagca ttcattcact tgttttttac ttccacttcg gctcgtatag tacaaatgca
     1321 ccgtatatgt gtagatgcaa gtaacattct tccggggagc atcttccggg agcaggaacg
     1381 aaaagcccaa acattatatc agaacagatc aaaagaactc ccacccatgc gctagcagat
//