From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: L-gulono-gamma-lactone oxidase [rats, Genomic, 996 nt, segment 12
Date: 27 Jan 93 05:48:12 GMT
Distribution: bionet
Checksum: 46215 4

LOCUS       M251632S12    996 bp    DNA             ROD       14-JAN-1993
DEFINITION  L-gulono-gamma-lactone oxidase [rats, Genomic, 996 nt, segment 12
            of 12] 
ACCESSION   S47516
KEYWORDS    .
SEGMENT     12 of 12
SOURCE      rats
  ORGANISM  Unclassified.
            Unclassified.
REFERENCE   1  (bases 1 to 996)
  AUTHORS   Nishikimi,M., Kawai,T. and Yagi,K.
  TITLE     Guinea pigs possess a highly mutated gene for
            L-gulono-gamma-lactone oxidase, the key enzyme for L-ascorbic acid
            biosynthesis missing in this species.
  JOURNAL   J. Biol. Chem. 267, 21967-21972 (1992)
  STANDARD  full automatic
COMMENT     This entry [NCBI gibbsq 116849] was created by the journal scanning
            component of NCBI/GenBank at the National Library of Medicine.
            This sequence comes from Fig. 3 and 4B.
FEATURES             Location/Qualifiers
     CDS             join(S47452:541..543,S47456:17..118,S47461:15..140,
                     S47808:16..110,S47476:17..107,S47477:17..202,
                     S47479:14..121,S47480:17..93,S47481:17..166,
                     S47514:17..181,S47515:17..107,17..145)
                     /note="GLO;  For the protein sequence (NCBI gibbsq
                     116851):  Method: conceptual translation supplied by
                     author.  This sequence comes from Fig. 3."
                     /product="L-gulono-gamma-lactone oxidase"
                     /codon_start=1
                     /translation="MVHGYKGVQFQNWAKTYGCSPEVYYQPTSVEEVREVLALAREQK
                     KKVKVVGGGHSPSDIACTDGFMIHMGKMNRVLQVDKEKKQITVEAGILLADLHPQLDE
                     HGLAMSNLGAVSDVTVAGVIGSGTHNTGIKHGILATQVVALTLMTADGEVLECSESRN
                     ADVFQAARVHLGCLGIILTVTLQCVPQFHLQETSFPSTLKEVLDNLDSHLKRSEYFRF
                     LWFPHTENVSIIYQDHTNKAPSSASNWFWDYAIGFYLLEFLLWTSTYLPCLVGWINRF
                     FFWMLFNCKKESSNLSHKIFTYECRFKQHVQDWAIPREKTKEALLELKAMLEAHPKVV
                     AHYPVEVRFTRGDDILLSPCFQRDSCYMNIIMYRPYGKDVPRLDYWLAYETIMKKFGG
                     RPHWAKAHNCTRKDFEEMYPTFHKFCDIREKLDPTGMFLNSYLEKVFY"
BASE COUNT      219 a    270 c    228 g    279 t
ORIGIN      
        1 ctgattgctt ccacaggccc acaattgcac ccggaaggac tttgaggaaa tgtaccccac
       61 ctttcacaag ttctgtgaca tccgtgagaa gctggacccc actggaatgt tcttgaattc
      121 gtacctggag aaagtcttct actaaagcag gagtggaaac aaaccaccct gacccctcac
      181 acttctgctg cccccggggg tctggggagc agagaagtgc ctcacaagca caatgggaac
      241 tgacctctcc tcctgaccac aaagaaaggc tgggctctgg gcgggtcctc tctgccttcg
      301 gcatcatttc ccttacatcc aggcaagaag tggcctctca ctcaaattcc tgttagcatt
      361 tccatgggtc acacataaac tgcaatcctc tcaggagaag ggggatccct gatacatcat
      421 atctatccag actaaggatg tggttcttcc tagattctat ggctccacca ggtatagaga
      481 gattcctggg gcctgcagtt ctccatccct cttcagaagg gagggatccc ttggcgagag
      541 tttggctcag aggtggcatg aagcatgctc tgctctctct tacccttgaa ggtccttcgg
      601 atgcccagag atgtctgctg gtcctgggca agccatcatt caaacgggtc caacctggcc
      661 ttctgtctgc catggcctga ccctcgcagt gtctcttcca gaggtgttta gagtggaact
      721 cgcttcaacc tcttaaccag ttgctgatcc ctgtgtttct ctcccttctc cttggagact
      781 actcttggag ggggatccca ccatgtcctt ggctttccct gggtattgtt ctcctcttcc
      841 tcttcacaaa tatgatttca gtttgatttg tggcctttct ggagtgttcc ttggagaacc
      901 aagatgttcc agctactttg taagaattaa aaggcactga agatgttttt ctgcccttga
      961 gaaattcata ttttaattct ttatctcata gagagt
//