From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: D.pseudoobscura Adh gene for alcohol dehydrogenase
Date: 28 Dec 92 21:27:13 GMT
Distribution: bionet
Checksum: 25106 8

LOCUS       DROADHGN42   3479 bp ds-DNA             INV       01-DEC-1992
DEFINITION  D.pseudoobscura Adh gene for alcohol dehydrogenase
ACCESSION   X62222
KEYWORDS    ADH gene; alcohol dehydrogenase.
SOURCE      Drosophila pseudoobscura DNA.
  ORGANISM  Drosophila pseudoobscura
            Eukaryota; Animalia; Metazoa; Arthropoda; Uniramia; Insecta;
            Pterygota; Neoptera; Holometabola; Diptera; Brachycera;
            Cyclorrhapha; Schizophora; Drosophiloidea; Drosophilidae.
REFERENCE   1  (bases 1 to 3479)
  AUTHORS   Schaeffer,S.W. and Miller,E.L.
  TITLE     Estimates of gene flow in Drosophila pseudoobscura determined from
            nucleotide sequence analysis of the alcohol dehydrogenase region
  JOURNAL   Genetics 132, 471-480 (1992)
  STANDARD  full automatic
COMMENT     *source: strain=MV28; *source: isolate=Mesa Verde, Colorado;
            *source: chromosome=IV section 88;
               From EMBL    entry DPADHGN42;  dated 16-OCT-1992.
FEATURES             Location/Qualifiers
     mRNA            join(40..119,917..1022,1087..1491,1552..1967)
                     /note="adult mRNA (Adh)"
     mRNA            join(884..1022,1087..1491,1552..1967)
                     /note="larval mRNA (Adh)"
     promoter        8..14
                     /note="adult promoter (Adh)"
     prim_transcript 40..1967
                     /note="adult primary transcript (Adh)"
     exon            40..119
                     /note="adult exon 1a"
     intron          120..916
                     /note="adult intron"
     promoter        848..855
                     /note="larval promoter (Adh)"
     prim_transcript 884..1967
                     /note="larval primary transcript (Adh)"
     exon            884..1022
                     /note="larval exon 1"
     exon            917..1022
                     /note="adult exon 1b"
     intron          1023..1086
                     /number=1
     exon            1087..1491
                     /number=2
     intron          1492..1551
                     /number=2
     exon            1552..1967
                     /number=3
     polyA_signal    1944..1949
     polyA_site      1967
     CDS             join(930..1022,1087..1491,1552..1818)
                     /EC_number="1.1.1.1"
                     /gene="Adh"
                     /product="alcoholdehydrogenase"
                     /codon_start=1
                     /translation="MSLTNKNVVFVAGLGGIGLDTSRELVKRNLKNLVILDRIDNPAA
                     IAELKAINPKVTITFYPYDVTVPVAETTKLLKTIFAQVKTIDVLINGAGILDDHQIER
                     TIAVNYTGLVNTTTAILDFWDKRKGGPGGIICNIGSVTGFNAIYQVPVYSGSKAAVVN
                     FTSSLAKLAPITGVTAYTVNPGITKTTLVHKFNSWLDVEPRVAEKLLEHPTQTSQQCA
                     ENFVKAIELNKNGAIWKLDLGTLEPITWTQHWDSGI"
BASE COUNT      971 a    793 c    798 g    917 t
ORIGIN      
        1 cgtagtgtat ttaaggcact gcacatgtcg aggaccacga ttattgtcta cagtgcagtc
       61 agtagttgtc aggtgcagca ggcggaccag catttcttta aatctactca atcgatcaag
      121 taagtaactg taatccgagt acagtcggcc cgtcccactc ggttctgccg taactcaaga
      181 aatttcttga tagcgcagtt ccagttgtaa aacgtcgttt aaaaaaacat ctcgttggag
      241 cttgggttga aaaacgttcg agttgggcgt agaaaatttc aaatataaaa tatcaaaaaa
      301 gagaaaatat cttcccaaag cagctgtcca ttttttgtta aagtgataga tcaaaaattc
      361 gtatgacgta tttcccaagt gctcttcatg caaataatgt cctcaattct aattctacaa
      421 gaagcacttt tggctcttgt ccagaaaaaa caatttgttt acacttttcg gttgataaca
      481 gaggcggacc gacggctaag taacataaca gtcgcctgtt aagtaatgcc ctcactctct
      541 gttagatggc cgcgcgtgtc gactccgata gaccagcaga cacgcagtag ttttcctcaa
      601 gaacctgaac tctaaacata gacataattt acgatacctt gagactcaaa taaaaataca
      661 agtatgagtg gtattctatt cctgattaga ctcgcacaca cacacacgta cgagtattaa
      721 cataaatttg ccggcaaata accaaaacaa aacgaacgca aacgatccga acacgtaatg
      781 cgagagataa gaaacagaaa agctcgacgt gaaagaagct tctggcggaa gctcttgccg
      841 aactccctat aaatacgaga cagaaaacca gaataaatat aataacaaac tgttgaattg
      901 tcccatcgat tcccagatca aaagacagaa tgtctctcac caacaagaac gtcgttttcg
      961 tggccggtct gggcggcatt ggcctggaca ccagccggga gttggtcaag cgtaatctga
     1021 aggtaagaga gaacgaattc catggaatct atggaatcct aaatttcaaa aattcattta
     1081 ttttagaacc tggtcatcct ggatcgcatt gacaatccgg ctgccattgc cgaactgaag
     1141 gcaatcaatc ccaaggtgac catcaccttc tatccctacg atgtgactgt gcccgtcgct
     1201 gagaccacca agctcctgaa gaccatcttt gcccaggtga agacaatcga tgtcctgatc
     1261 aacggtgctg gcatcctgga cgatcatcag atcgagcgca ccattgccgt taactacacg
     1321 ggcctggtca acaccaccac agccattctg gacttctggg acaagcgcaa gggcggccca
     1381 ggcggcatca tttgcaacat tggctccgtc accggtttca atgccatcta ccaggtgccc
     1441 gtttactccg gctccaaggc ggcggtggtt aactttacct cctccctggc ggtaagtaat
     1501 ccaacgtttt tcctactccc caaaactaaa tatttctttg aaatcccata gaaactggct
     1561 cccattactg gtgtcactgc ttacactgtc aatcctggca tcactaagac cactctggtc
     1621 cacaaattca actcgtggct ggatgtggag ccccgtgtgg cagagaagct gctcgagcat
     1681 cccacccaga cctctcagca gtgcgccgag aactttgtga aggccatcga gctgaacaag
     1741 aacggtgcca tctggaagtt ggatttgggc accttggagc ccatcacatg gacccagcac
     1801 tgggactctg gcatctaaac catcccagag actctatggg acatggcgtt ttagctttta
     1861 gtttcttttt ttccactcaa ttgttacgta tatacataca catatggcaa taaggctgat
     1921 ttgaaccccg ttttgaatat gataatacaa aattatattt gagaaaattc aacaaaatcg
     1981 ataagagcta tagctctctt ttaattaaaa aaaactagat aaaaagagca atggtcagtg
     2041 gtggtcagtg gtcgttgtgg cctctatcga tttccacaca aaaactttac tgtttagtaa
     2101 tagaaaagaa cttgagaaag gcagccaaaa gaatgtacga tctgacgggt aagcatgtct
     2161 gctatgtggc tgactgcggt ggcattgcac tggagaccag caaggttctc atgaccaaga
     2221 atatagcggt gagtggtaga gaggtggtgt agagagtgga gagtggttct ggttcttaaa
     2281 caagtctcta gtctctagtc tctagtctct tggctgctgc tggtagctgt cgaaactaat
     2341 taagttaatg actttttcat gttttgtttt tgtccgctga cttttgctgt tcaaagctac
     2401 aattaagttt atgttttgag tttgatctac ttttgcttaa gggaagtact ttcgattacg
     2461 gattgctggc atataaaaat agataccaag gaatgtgcat catatcatat tgattctatc
     2521 ttgattcttt gcccagaaac tggccatcct tcagagcgtg gaaaaccccc cggccatcgc
     2581 tcagttacaa tcgattaagc acagcacaca gatcttcttc tggaccttcg atgtgaccat
     2641 ggcccgcgag gagatgaaga agtacttcga tgaggtcatg gtccagatgg actacatcga
     2701 tgtgctgatc aatggggcca ccctgtgcga tgagcggaac attgatgcca ccatcaatac
     2761 gaatctgacc ggcatgatga acaccgtggc cactgtgctg ccctatatgg accgtaagat
     2821 gggcggttcg ggtggactga ttgtgaatgt cacctccgtc ataggactgg atccatcgcc
     2881 agtcttttgt gcgtacagtg cctcaaagtt tggtgtgatt gggttcaccc gaagtctagc
     2941 ggtgagtggg cgagatctta acttgaaaat gctctctaat gtcttccatt ctttcgcagg
     3001 atcccctgta ttacacccaa aatggtgtgg ctgtcatggc cgtctgctgt ggtcccacca
     3061 aagtgtttgt cgatcgggaa ctgactgcct tcctggaata cggccagtcc tttgccgatc
     3121 gcctgcgtcg tgccccctgc caatcgacgg ccgtctgcgg ccagaatata gtaaatgcca
     3181 tcgagagatc ggagaacgga cagatctgga ttgccgacaa gggcggcctg gagtcggtgg
     3241 cccttcactg gtattggcat atggccgatc agtttgtgaa ctatatgcag agcactgacg
     3301 atgaggatca ggagtttttt ttggggcagc gatgaggcat gccttgatcc acggaataaa
     3361 acatcaaatg tgtcacaaaa agtaggcaac ttgtttgtgg gtttggaaat agatttgaaa
     3421 tattttgagg tcatatgaat aatgacataa atgagggtgc ctgaaagtat gccaagttt
//