From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Brassica napus isocitrate lyase (ILA133) gene, complete cds.
Date: 13 Jan 93 05:46:00 GMT
Distribution: bionet
Checksum: 12666 9

LOCUS       BNAICL       4373 bp ds-DNA             PLN       12-JAN-1993
DEFINITION  Brassica napus isocitrate lyase (ILA133) gene, complete cds.
ACCESSION   L08482
KEYWORDS    isocitrate lyase.
SOURCE      Brassica napus DNA.
  ORGANISM  Brassica napus
            Eukaryota; Plantae; Embryobionta; Magnoliophyta; Magnoliopsida;
            Dilleniidae; Capparales; Brassicaceae.
REFERENCE   1  (bases 1 to 4373)
  AUTHORS   Zhang,J.Z., Gomez-Pedrozo,M., Baden,C.S. and Harada,J.J.
  TITLE     Two classes of isocitrate lyase genes are expressed during late
            embryogeny and postgermination in Brassica napus L
  JOURNAL   Mol. Gen. Genet. (1993) In press
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     exon            1..107
                     /number=1
                     /gene="ILA133"
                     /evidence="EXPERIMENTAL"
                     /citation=[1]
     intron          108..214
                     /number=1
                     /gene="ILA133"
                     /evidence="EXPERIMENTAL"
                     /citation=[1]
     exon            215..621
                     /number=2
                     /gene="ILA133"
                     /evidence="EXPERIMENTAL"
                     /citation=[1]
     intron          622..1338
                     /number=2
                     /gene="ILA133"
                     /evidence="EXPERIMENTAL"
                     /citation=[1]
     exon            1339..1941
                     /number=3
                     /gene="ILA133"
                     /evidence="EXPERIMENTAL"
                     /citation=[1]
     intron          1942..3005
                     /number=3
                     /gene="ILA133"
                     /evidence="EXPERIMENTAL"
                     /citation=[1]
     exon            3006..3598
                     /number=4
                     /gene="ILA133"
                     /evidence="EXPERIMENTAL"
                     /citation=[1]
     intron          3599..4017
                     /number=4
                     /gene="ILA133"
                     /evidence="EXPERIMENTAL"
                     /citation=[1]
     exon            4018..4373
                     /number=5
                     /gene="ILA133"
                     /evidence="EXPERIMENTAL"
                     /citation=[1]
     CDS             join(78..107,215..621,1339..1941,3006..3598,4018..4115)
                     /EC_number="4.1.3.1"
                     /gene="ILA133"
                     /product="isocitrate lyase"
                     /codon_start=1
                     /translation="MAASFSVPSMIMEEEGRFEAEVAEVQTWWSSERFKLTRRPYTAR
                     DVVALRGHLKQGYASNEMAKKLWRTLKSHQANGTASRTFGALDPVQVTMMAKHLDTIY
                     VSGWQCSSTHTSTNEPGPDLADYPYDTVPNKVEHLFFAQQYHDRKQREARMSMSREER
                     AKTPFVDYLKPIIADGDTGFGGTTATVKLCKLFVERGAAGVHIEDQSSVTKKCGHMAG
                     KVLVAVSEHINRLVAARLQFDVMGTETVLVARTDAVAATLIQSNIDSRDHQFILGVTN
                     PSLRGKSLSSLLAEGMAVGNNGPALQAIEDQWLSSARLMTFSDAVVEALKRMNLSENE
                     KSRRVNEWLNHARYENCLSNEQGRELAAKLGVTDLFWDWDLPRTREGFYRFQGSVTAA
                     VVRGWAFAQIADLIWMETASPDLNECTQFAEGVKSKTPEVMLAYNLSPSFNWDASGMT
                     DQQMMEFIPRIARLGYCWQFITLAGFHADALVVDTFAKDYARRGMLAYVERIQREERS
                     NGVDTLAHQKWSGANYYDRYLKTVQGGISSTAAMGKGVTEEQFKETWTRPGAAGMGEG
                     TSLVVAKSRM"
BASE COUNT     1264 a    840 c    950 g   1319 t
ORIGIN      
        1 agcaacatgc ttgaagtttc tctactttca taagtcctta aaagaaaaat catttccaat
       61 tcataaaatt tgaagccatg gctgcatctt tctctgtccc ctctatggta tagcaacact
      121 aaatcacctc atcaatgttc cgttaagata tttttagtta tttaattatg taggttaaca
      181 acattggcta agtccattgc gaatgatata tcagatcatg gaagaggaag ggagattcga
      241 ggcggaagtt gcggaagtgc agacttggtg gagctcagag aggttcaagc taacaaggcg
      301 tccttacacg gcccgtgacg tggtggctct acgtggccat ctcaagcaag gttatgcttc
      361 gaacgagatg gctaagaagc tgtggagaac gctcaagagt caccaagcca acggcacggc
      421 gtctcgcacg tttggtgcct tggaccctgt tcaggtgaca atgatggcta aacatttgga
      481 caccatttat gtctctggtt ggcagtgctc gtctactcac acctccacta acgagcctgg
      541 tccggatctt gctgactatc catacgatac cgttcctaac aaagtcgaac atctcttctt
      601 cgctcagcag taccatgaca ggtaattttg gttactaata aaatattgta ttgtaattgt
      661 actatggctg atggtatata agtttcttca ctactacaac cttgatattt gctgcatggt
      721 atgggagtaa gttctttttt tttttctttt ttttttgaat accaaattat attaaattaa
      781 aacccaatgg gctatgggag taagttctga acgtagaaaa tagcgtgaaa attttctctc
      841 tgttaggtta atgtagtgtg aactctggtc gcggatagct cgtgtcaaaa atagttaagt
      901 aattatattt tatggctttc ggtgtcttgg ttacgaaatt gcttctttcg aggatttacg
      961 ttgccacgtc ttacgataat ttgatcgtct gtcaaactat agtttataca cgttctagtt
     1021 gagatcaaat ttacaatgtg aattcttgga aataacatga tagaatactt attttcttcg
     1081 gtaaggaaat attaaatttg gaccaatcgg cctatttagc attcaaaaat atattaagag
     1141 accattaccg aaatcagtta tggatagtgt gaagtgtttt gatcgttaga tttgtaacgt
     1201 tgcatgcagc ctgttttaat cccaaaaata catttttaga ccattaaatc caatggataa
     1261 tgttagacat ttaggtgtgt tttattaaca ctaggataat aattatctaa ttacagcatc
     1321 gatacgtctc tctcgcagaa aacagaggga ggcgagaatg agcatgagca gagaagaaag
     1381 agcaaaaact ccgtttgtgg actacttgaa gcccatcatc gccgacggag acaccggttt
     1441 cggtggcact accgccaccg tcaaactctg taagctcttc gtcgagagag gcgcagctgg
     1501 ggtccacatc gaggaccagt cctccgtcac caagaagtgt ggccacatgg cgggaaaagt
     1561 cctcgtggcg gtaagtgaac acattaaccg ccttgtcgcg gctcggctcc agttcgatgt
     1621 gatgggcaca gagaccgttc tggtcgctag aacggatgcg gtggcggcta ctttgatcca
     1681 gtcgaacatt gactcgaggg accatcagtt catcctcggt gtcactaacc cgagtcttag
     1741 aggcaagagt ttgtcctcgc ttctggccga gggaatggct gtaggcaata atggtccagc
     1801 gttgcaagcg attgaggatc aatggcttag ctcagctcgt ctcatgactt tctcggacgc
     1861 tgtcgtggag gctctcaagc gcatgaacct aagtgagaat gagaagagcc ggagagtgaa
     1921 cgagtggcta aaccatgcaa ggtaatgaat aagaatttta gtagtacatc cgaaaatatc
     1981 atatatcttt ggttaactgt tatagacttc tagtttgaaa ctatttatca aaactaacaa
     2041 ttttacccaa attaagaatt tggttttgtg taggttctag ttatgataat caaataatga
     2101 agcggtagta tttaatctca aatacaaaat ttagttagat ttaagtttac caactaaata
     2161 attattttgg tgcgctagtt acaaagttaa gaaccgtctc ttaaataaga tatataagag
     2221 catctttaac cctaagacca tgattatcgc tgcacggttt cataggtcga tctttaattt
     2281 tttttttgtt tctgctgatt aaaaaaaaaa ttagaaatcg aaccaatcgc gggccgccac
     2341 gtgtcagtgg ggcccgcgaa cagtgcaaga attgcaccaa agtcgatcat tgtttaacct
     2401 cttctatcac cgtttttaac acaaaaggtg ggacctccaa cttaaaaacc ccacttatat
     2461 cctccgataa ttctgcccta acttaatttt ttttttttct gatttaaaaa aaaaaaaatt
     2521 aaaaactaac caatcgtggg ccgcacgtgt cagtggggcc cgcaaacagt gcaagaacca
     2581 agctcttaga gaaagggttt tgagcatgat tattggaggg ttctttctta gtttttttag
     2641 ttaaaagtta agagacgggt tcttatattc cgctaagaac cccgcaataa tcatgttttt
     2701 aggagccaac tcttaagttt tttgtgggtc tcacctctta agaacccttt taagaggtgg
     2761 agataatcat gctctaagag ccgtctctta gccaaaaaat gtttttaaaa aagtgtcaaa
     2821 tcatgagtta ataaacccag gtaagagacc ggagttaatc atgctcttaa ggtttttcac
     2881 aaacaatatt cgtatctaat gggcatatct tattcctatt tataaagaaa cttttcaatg
     2941 ttgatgttta tcaaaagtac agtcttacac tagctaatga aaatgaaacc cattggacta
     3001 atcaggtacg agaactgcct gtcaaacgag caaggccgag aattagcagc aaaactcggt
     3061 gtgactgatc ttttctggga ctgggacttg cccagaacca gagaaggatt ctaccggttc
     3121 caaggctcgg tcacagcagc cgtggtccgt ggctgggcct ttgcacagat agctgatctc
     3181 atctggatgg aaaccgcaag ccctgacctc aacgaatgca cccaattcgc agaaggagtc
     3241 aagtccaaga caccagaggt aatgctcgcc tacaacctct ccccatcctt caactgggac
     3301 gcttctggta tgacggatca gcagatgatg gagttcattc cacgaatcgc caggctcggt
     3361 tattgctggc agtttataac ccttgcgggt ttccatgcgg atgctcttgt ggtcgatacg
     3421 tttgcaaagg attacgcgag gagagggatg ctggcttatg tcgagaggat acagagagaa
     3481 gagaggagca atggggttga cacattggct catcagaaat ggtcaggtgc taattactat
     3541 gatcgttatc ttaagaccgt ccaaggtgga atctcctcca ctgcagccat gggcaaaggt
     3601 aatattactt tttttgttta atttgctcac acactttata ataaataaat tagagtttgt
     3661 gatgggaaat agttatttat agtatgggga atttgcacac aaatagtttt tggattcagt
     3721 cttaagatct tccacttctt aggataaata aaagatgctt acacatgtta catattttta
     3781 gatcgcccaa tcataataat tcagttgttg aagattcgtc cggttggtcg agacaagtga
     3841 tctcttctat tgaacatata taatagtcac tgtagatact taaaatgcct tgattagtaa
     3901 gaaaaaatct gacttttaca taacattatg catgcagtta ccgttcgctc gtttcattat
     3961 gcatccaagt ataagctttt tttttcattt cctcaaatga tgtgttttgg catgtaggtg
     4021 ttaccgagga acaattcaaa gagacctgga cgaggccggg agctgctgga atgggcgaag
     4081 ggactagcct tgtggtggcc aagtccagaa tgtaagacag acgttcattg cggcggagcg
     4141 gccaaggcgg ttcggcatct tcgcagaaaa acttggaaac caccttatct ttattatatc
     4201 tttactcgag taaaagaaaa agacataata cttgtatctt cgttattgtg ttcgctttct
     4261 tctgcgtaat aattgggttg aaaagtttgg ggcaatattt acggggtttg cttctttgta
     4321 tggatatttg gaatttggtg tttttggctt tcattataaa atgatttcat att
//