From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: D.pseudoobscura Adh gene for alcohol dehydrogenase
Date: 28 Dec 92 21:28:07 GMT
Distribution: bionet
Checksum: 46042 8

LOCUS       DROADHGN78   3474 bp ds-DNA             INV       01-DEC-1992
DEFINITION  D.pseudoobscura Adh gene for alcohol dehydrogenase
ACCESSION   X64487
KEYWORDS    ADH gene; alcohol dehydrogenase.
SOURCE      Drosophila pseudoobscura DNA.
  ORGANISM  Drosophila pseudoobscura
            Eukaryota; Animalia; Metazoa; Arthropoda; Uniramia; Insecta;
            Pterygota; Neoptera; Holometabola; Diptera; Brachycera;
            Cyclorrhapha; Schizophora; Drosophiloidea; Drosophilidae.
REFERENCE   1  (bases 1 to 3474)
  AUTHORS   Schaeffer,S.W. and Miller,E.L.
  TITLE     Estimates of gene flow in Drosophila pseudoobscura determined from
            nucleotide sequence analysis of the alcohol dehydrogenase region
  JOURNAL   Genetics 132, 471-480 (1992)
  STANDARD  full automatic
COMMENT     *source: strain=PS287; *source: isolate=Kiabab National Forest,
            Arizona; *source: chromosome=IV section 88;
               From EMBL    entry DPADHGN78;  dated 16-OCT-1992.
FEATURES             Location/Qualifiers
     mRNA            join(40..119,913..1018,1082..1486,1547..1962)
                     /note="adult mRNA (Adh)"
     mRNA            join(880..1018,1082..1486,1547..1962)
                     /note="larval mRNA (Adh)"
     promoter        8..14
                     /note="adult promoter (Adh)"
     prim_transcript 40..1962
                     /note="adult primary transcript (Adh)"
     exon            40..119
                     /note="adult exon 1a"
     intron          120..912
                     /note="adult intron"
     prim_transcript 880..1962
                     /note="larval primary transcript (Adh)"
     exon            880..1018
                     /note="larval exon 1"
     exon            913..1018
                     /note="adult exon 1b"
     intron          1019..1081
                     /number=1
     exon            1082..1486
                     /number=2
     intron          1487..1546
                     /number=2
     exon            1547..1962
                     /number=3
     polyA_signal    1939..1944
     polyA_site      1962
     CDS             join(926..1018,1082..1486,1547..1813)
                     /EC_number="1.1.1.1"
                     /gene="Adh"
                     /product="alcoholdehydrogenase"
                     /codon_start=1
                     /translation="MSLTNKNVVFVAGLGGIGLDTSRELVKRNLKNLVILDRIDNPAA
                     IAELKAINPKVTITFYPYDVTVPVAETTKLLKTIFAQVKTIDVLINGAGILDDHQIER
                     TIAVNYTGLVNTTTAILDFWDKRKGGPGGIICNIGSVTGFNAIYQVPVYSGSKAAVVN
                     FTSSLAKLAPITGVTAYTVNPGITKTTLVHKFNSWLDVEPRVAEKLLEHPTQTSQQCA
                     ENFVKAIELNKNGAIWKLDLGTLEPITWTQHWDSGI"
BASE COUNT      968 a    792 c    796 g    918 t
ORIGIN      
        1 cgtagtgtat ttaaggcact gcacatgtcg aggaccacga ttattgtcta cagtgcagtc
       61 agtagttgtc aggtgcagca ggcggaccag catttcttta aatctactca atcgatcaag
      121 taagtaactg taatccaagt acagtcggcc catcccactc ggttctgccg taactcaaga
      181 aatttcttga tagcgcagtt ccagttgtag aacgtcattt aaaaaaacat ctcgttggag
      241 cttgggttga aaaacgttcg agttgggcgt agaaaatttc aaatataaaa tatcaaaaaa
      301 gagaaaatat tctcccaaag cagctgtcca ttttttgtta aagtgataga tcaaaaattc
      361 gattgacgta tttcccaagt gctctttatg caaataattt cctcaattct aattctacaa
      421 gaagcacttt tggctcttgt ccagaaaaaa caatttgttt acacttttcg gttgataaca
      481 gaggcggacc gacggctaag taacataaca gtcgcctgtt aagtaatgcc ctcactctct
      541 gttagatggc cgcgcgtgtc gactccgata gaccagcaga cacgcagtag ttttcctcaa
      601 gaacctgaac tctaaacata gacataattt acgatacctt gagactcaaa taaaaataca
      661 agtatgagtg gtattctatt cctgattaga ctcgcacaca cacgtacgag tattaacata
      721 aatttgccgg caaataacca aaacaaaacg aacgcaaacg atccgaacac gtaatgcgag
      781 agataagaaa cagaaaagct cgacgtgaaa gaagcttctg gcggaagctc ttgccgaact
      841 ccctataaat acgagacaga aaaccagaat aaatataata acaaactgtt gaattgtccc
      901 atcgattccc agatcaaaag acagaatgtc tctcaccaac aagaacgtcg ttttcgtggc
      961 cggtctgggc ggcattggcc tggacaccag ccgggagttg gtcaagcgta atctgaaggt
     1021 aagagtgaac gaattccatg gaatctatgg aatcctaaat ttaaaaattc atttatttta
     1081 gaacctggtc atcctggatc gcattgacaa tccggctgcc attgccgagc tgaaggcaat
     1141 caatcccaag gtgaccatca ccttctatcc ctacgatgtg actgtgcccg tcgctgagac
     1201 caccaagctc ctgaagacca tctttgccca ggtgaagaca atcgatgtcc tgatcaacgg
     1261 tgctggcatc ctggacgatc atcagatcga gcgcaccatt gccgttaact acacgggcct
     1321 ggtcaacacc accacagcca ttctggactt ctgggacaag cgcaagggcg gcccaggcgg
     1381 catcatttgc aacattggat ccgtcaccgg tttcaatgcc atctaccagg tgcccgttta
     1441 ctccggctcc aaggcggcgg tggttaactt tacctcctcc ctggcggtaa gtaatccaac
     1501 ctttttccta ctccccaaaa ctaaatattt ctttgaaatc ccatagaaac tggctcccat
     1561 tactggtgtc actgcttaca ctgtcaatcc tggcatcact aagaccactc tggtccacaa
     1621 attcaactcg tggctggatg tggagccccg tgtggcggag aagctgctcg agcatcccac
     1681 ccagacctct cagcagtgcg ccgagaactt tgtgaaggcc attgagctga acaagaacgg
     1741 tgccatctgg aagttggatc tgggcacctt ggagcccatc acatggaccc agcactggga
     1801 ctcgggcatc taaaccatcc tagagactct atgggacatg gcgttttagc ttttagtttc
     1861 tttttttcca ctcaattgtt acgtatatac atacacatat ggcaataagg ctgatttgaa
     1921 ccccgttttg aatatgataa tacaaaatta tatttgagaa atttcaacaa aatcgataag
     1981 agctatagct ctcttttaat taaaaaaaac tagataaaaa gagcaatggt cagtggtggt
     2041 cggtggtcgt tgtggcctct atcgatttcc acacaaaaac tttactgttt agtaatagaa
     2101 aagaacttga gaaaggcagc caaaagaatg tacgatctga cgggtaagca tgtctgctat
     2161 gtggctgact gcggtggcat tgcactggag accagcaagg ttctcatgac caagaatata
     2221 gcggtgagtg gtggagaggt ggtgtagaga gtggagagtg gttctggttc ttaaacaagt
     2281 ctctagtctt tagtctctag tctcttggct gctgctggta gctgtcgaaa ctaattaagt
     2341 taatgacttt ttcatgtttt gtttttgtcc gctgactttt gctgttcaaa gctacaatta
     2401 agtttatgtt ttgagtttga tctacttttg cttaagggaa gtactttcga ttacggattg
     2461 ctggcatata aaaatagata ccaaggaatg tgcctcatat catattgatt ctatcttgat
     2521 tctttgccca gaaactggcc atccttcaga gcgtggaaaa ccccccggcc atcgctcagt
     2581 tacaatcgat taagcacagc acacagatct tcttctggac cttcgatgtg accatggccc
     2641 gcgaggagat gaagaagtac ttcgatgagg tcatggtcca gatggactac atcgatgtac
     2701 tgatcaatgg ggccaccctg tgcgatgagc ggaacattga tgccaccatc aatacgaatc
     2761 tgaccggcat gatgaacacc gtggccactg tgctgcccta tatggaccgt aagatgggcg
     2821 gttcgggtgg actgattgtg aatgtcacct ccgtcatagg actggatcca tcgccagtct
     2881 tttgtgcgta cagtgcctca aagtttggtg tgattgggtt cacccgaagt ctagcggtga
     2941 gtgggcgaga tcttaacttg aaaatgctct ctaatatctt ccattcttcc gcaggatccc
     3001 ctgtattaca cccaaaatgg tgtggctgtc atggccgtct gctgtggtcc caccaaagtg
     3061 tttgtcgatc gggaactgac tgccttcctg gcatacggcc aaacctttgc cgatcgcctg
     3121 cgtcgtgccc cctgccaatc gacggccgtc tgcggccaga atatagtaaa tgccatcgag
     3181 agatcggaga acggacagat ctggattgcc gacaagggcg gcctggagtc ggtggccctt
     3241 cactggtatt ggcatatggc cgatcagttt gtgaactaca tgcagagcac tgacgatgag
     3301 gatcaggagt tttttttggg gcagcgatga ggcatgcctt gatccacgga ataaaacatc
     3361 aaatgtgtca caaaaagtag gcaacttgtt tgtgggtctg gaaatagatt tgaaatattt
     3421 tgaggtcata tgaataatga cataaatgag ggtgcctgaa agtatgccaa gttt
//