From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: C.fasciculata gene encoding trypanothione reductase.
Date: 25 Jul 92 10:07:15 GMT
Distribution: bionet
Checksum: 62710     6

LOCUS       TRFTRG       1771 bp ds-DNA             INV       25-JUL-1992
DEFINITION  C.fasciculata gene encoding trypanothione reductase.
ACCESSION   Z12618
KEYWORDS    trypanothione reductase.
SOURCE      Crithidia fasciculata DNA.
  ORGANISM  Crithidia fasciculata
            Eukaryota; Animalia; Protozoa; Sarcomastigophora; Mastigophora;
            Kinetoplastida; Trypanosomatina; Trypanosomatidae.
REFERENCE   1  (bases 1 to 1771)
  AUTHORS   Fairlamb,A.H.
  JOURNAL   Unpublished (1991)
  STANDARD  full staff_review
REFERENCE   2  (sites)
  AUTHORS   Hunter,W.N., Bailey,S.E., Habash,J., Harrop,S.J., Helliwell,J.R.,
            Aboagye-Kwarteng,T., Smith,K. and Fairlamb,A.H.
  TITLE     The active site of trypanothione reductase: a target for rational
            drug design.
  JOURNAL   J. Mol. Biol. (1992) In press
  STANDARD  full staff_review
REFERENCE   3  (bases 1 to 1771)
  AUTHORS   Aboagye-Kwarteng,T., Smith,K. and Fairlamb,A.H.
  TITLE     Molecular characterization of the trypanothione reductase gene from
            Crithidia fasciculata and Trypanosoma brucei: comparison with other
            flavoprotein disulphide oxidoreductases with respect to ...
  JOURNAL   Mol. Microbiol. (1992) In press
  STANDARD  full automatic
COMMENT     *source: strain=Wild type; *source: clone_library=EMBL3; *source:
            clone=pKSTR; *source: isolate=HS6;
            From EMBL    entry CFTRG;  dated 17-JUN-1992.
FEATURES             Location/Qualifiers
     CDS             111..1586
                     /function="Flavoproteindisulphide oxidoreductase"
                     /EC_number="1.6.4.8"
                     /standard_name="TR"
                     /note="Active-site cysteinedisulphide: cys52 and cys57.
                     Active site base:his461. Homodimer. FAD co-enzyme.
                     Cytosolicprotein. Similarity to glutathione reductase
                     anddihydrolipoamide dehydrogenase."
                     /product="trypanothione reductase"
                     /evidence=EXPERIMENTAL
                     /codon_start=1
                     /translation="MSRAYDLVVIGAGSGGLEAGWNAASLHKKRVAVIDLQKHHGPPH
                     YAALGGTCVNVGCVPKKLMVTGANYMDTIRESAGFGWELDRESVRPNWKALIAAKNKA
                     VSGINDSYEGMFADTEGLTFHQGFGALQDNHTVLVRESADPNSAVLETLDTEYILLAT
                     GSWPQHLGIEGDDLCITSNEAFYLDEAPKRALCVGGGYISIEFAGIFNAYKARGGQVD
                     LAYRGDMILRGFDSELRKQLTEQLRANGINVRTHENPAKVTKNADGTRHVVFESGAEA
                     DYDVVMLAIGRVPRSQTLQLDKAGVEVAKNGAIKVDAYSKTNVDNIYAIGDVTDRVML
                     TPVAINEGAAFVDTVFANKPRATDHTKVACAVFSIPPMGVCGYVEEDAAKKYDQVAVY
                     ESSFTPLMHNISGSTYKKFMVRIVTNHADGEVLGVHMLGDSSPEIIQSVAICLKMGAK
                     ISDFYNTIGVHPTSAEELCSMRTPAYFYQKGKRVEKIDSNL"
                     /citation=[2]
                     /citation=[1]
BASE COUNT      362 a    578 c    548 g    283 t
ORIGIN
        1 cgcctctacg cacagactca cacactctct agcgctctcc ccctcccgac ctcactcgca
       61 acacccacac ccaaaccgtc tcgaaaattc tccgccactc cgcccgactg atgtcccgcg
      121 cgtacgacct ggtggtgatc ggcgccggct ccggcggcct ggaggcgggc tggaacgcgg
      181 cgtcgctgca taagaagcgg gtggcggtga tcgacctgca gaagcaccac ggcccgccgc
      241 actacgccgc gctcggcggc acgtgcgtga acgtgggctg cgtccccaag aagctgatgg
      301 tgaccggcgc caactacatg gacaccatcc gcgagtccgc cggcttcggc tgggagctgg
      361 accgcgagtc ggtgcggccg aactggaagg cgctgatcgc cgccaagaac aaggcggtgt
      421 cggggatcaa cgacagctac gagggcatgt tcgccgacac agagggcctt accttccacc
      481 agggcttcgg cgcgctgcag gacaaccaca cagtgctcgt gcgtgagtcg gcggacccga
      541 acagcgcggt gctggagacg ctggacaccg agtacatcct gttggccacc ggctcgtggc
      601 cgcagcacct cggcatcgag ggcgacgacc tgtgcatcac cagcaacgag gccttctacc
      661 tggacgaggc cccgaagcgc gcgctgtgcg tcggaggcgg ctacatctcg atcgagttcg
      721 ccggcatctt caacgcgtac aaggcgcgcg gcggccaggt ggacctggcg taccgcggcg
      781 acatgatcct gcgcggcttc gactcggagc tgcgcaagca gctgacggag cagctgcgcg
      841 ccaacggcat caacgtgcgc acgcacgaga acccggcaaa ggtgaccaag aacgcggacg
      901 gcacgcgcca cgtggtgttc gagagcggcg cggaggcgga ctacgacgtg gtgatgctgg
      961 ccatcggccg cgtgccgcgc tcgcagacgc tgcagctgga caaggccggc gtggaggtgg
     1021 ccaagaacgg cgccatcaag gtggacgcct actccaagac caacgtggac aacatctacg
     1081 ccatcggcga cgtcacggac cgggtcatgc tgacgcccgt cgccatcaac gaaggtgccg
     1141 ccttcgtgga caccgtcttc gccaacaagc cgcgcgccac cgaccacacg aaggtggcct
     1201 gcgccgtctt ctccattccg cccatgggcg tgtgcggcta cgtcgaggag gacgcggcca
     1261 agaagtacga ccaggtggcg gtgtacgaga gcagcttcac gccgctcatg cacaacatca
     1321 gcggcagcac gtacaagaag ttcatggtgc gcatcgtcac caaccacgcc gacggtgagg
     1381 tgctgggcgt gcacatgctg ggcgacagct cgccggagat catccagagc gtggccatct
     1441 gcctgaagat gggtgccaag atcagcgatt tctacaacac catcggcgtt cacccaacca
     1501 gcgccgagga gctgtgctcg atgcgcacgc cggcctactt ttaccagaag ggcaagcgcg
     1561 tggagaagat cgacagcaac ctgtaagcgg tgaagcaccc gacaaagggt gaaaaatacg
     1621 tggacccgaa gctctcgttg ccgcacatca tcttgataag tgcgcaatcg tctattatga
     1681 ggaagagaaa gcataaagcc ccttctctta tcttccttct cttcaatttt ttctttttcg
     1741 agctcttcgc ggtcaaattt cgtgctttcg t
//