From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Mouse mammary tumor virus (proviral), 5' UT and half of gag gene.
Date: 22 Dec 92 22:55:18 GMT
Distribution: bionet
Checksum: 37072 5

LOCUS       MMT5EG       1371 bp ss-RNA             VRL       30-NOV-1992
DEFINITION  Mouse mammary tumor virus (proviral), 5' UT and half of gag gene.
ACCESSION   X00018
KEYWORDS    gag protein; long terminal repeat (LTR); polyprotein;
            repetitive sequence; terminal repeat.
SOURCE      Mouse mammary tumor virus (strain GR), cDNA to viral RNA, clone H.
  ORGANISM  Mouse mammary tumor virus
            Viridae; ss-RNA enveloped viruses; Positive strand RNA virus;
            Retroviridae; Oncovirinae; Type B oncovirus group; Mammalian type B
            oncoviruses.
REFERENCE   1  (bases 1 to 1371)
  AUTHORS   Fasel,N., Buetti,E., Firzlaff,J., Pearson,K. and Diggelmann,H.
  TITLE     Nucleotide sequence of the 5' noncoding region and part of the gag
            gene of mouse mammary tumor virus; identification of the 5'
            splicing site for subgenomic mRNAs
  JOURNAL   Nucleic Acids Res. 11, 6943-6955 (1983)
  STANDARD  full automatic
COMMENT     The mRNA exon/intron boundary may be at base 293 instead of 289.
            [1].
               This proviral sequence appears to be colinear with viral GR-RNAs
            isolated from infected cells [1].

              EMBL features not translated to GenBank features:
              key        from     to       description
              CAP           1      1       cap site
              CDS         313  >1371       gag precursor polypeptide
              SITE        288    288       putative 5' splicing site
              SITE        435    452       repetitive sequence
              MSG           1  >1371       messenger RNA.
FEATURES             Location/Qualifiers
     mRNA            <288..288
                     /note="env mRNA (5' end putative); putative"
     LTR             <1..133
                     /note="5' LTR"
     intron          289..>289
                     /note="env intron A"
     CDS             313..>1371
                     /note="gag precursor polypeptide"
                     /codon_start=1
                     /translation="MGVSGSKGQKLFVSVLQRLLSERGLHVKESSTIEFYQFLIKVSL
                     GFPKKEDLNLQDWKRVGREMKKYAADDGTDSIPKQAYPIWLQLREILTEQSDLVLLSA
                     EAKSVTEEELEEGLTGLLSASSQEKTYGTRGTAYAEIDTEADKLSEHIYDEPYEEKEK
                     ADKNEEKDHVRKVKKIVQRKENSEHKRKEKDQKAFLATDWNDDDLSPEDWDNLEEQAA
                     HYHDDDELILPVKRKVVKKKPLALRRKPLPPVGFAGAMAEAREKGDLTFTFPVVFMGE
                     SDDDDTPVWEPLPLKTLKELQSAVRTMGPSAPYTLEVVDMVASQWLTPSDWHQTARAT
                     LSPGDYVLWRTEYEEKSKE"
BASE COUNT      419 a    269 c    354 g    329 t
ORIGIN      134 bp upstream of PvuII site; 5' end of viral RNA.
        1 gcaacagtcc taacattctt ctctcgtgtg tttgtgtctg ttcgccatcc cgtctccgct
       61 cgtcacttat ccttcacttt tcagagggtc cccccgcaga tcccggtcac cctcaggtcg
      121 gccgactgcg gcagctggcg cccgaacagg gaccctcgga taagtgaccc ttgtctttat
      181 ttctactatt ttgtgttcgt cttgttttgt ctctatcttg tctggctatc atcacaagag
      241 cggaacggac tcaccacagg gaactgcagt ctcgcctacg gagaagaggt aggttacggt
      301 gtgccattgg atatgggggt ctcgggctca aaagggcaga aactctttgt ttctgtttta
      361 caaaggctcc tctcagagag gggtcttcat gtgaaggaga gcagcacaat agagttttat
      421 cagtttctga taaaggtttc ccttggtttc ccgaagaagg aggacttgaa tttacaagat
      481 tggaaaaggg taggaagaga aatgaagaaa tatgcagcgg acgatgggac agatagcata
      541 ccaaagcagg cctaccccat ttggcttcag ttaagagaga tattaacaga acagtcagat
      601 ttggtcttac tatccgctga ggccaagtct gtgactgaag aagagttaga agaaggttta
      661 accgggctac tatcagcaag ttcacaagaa aaaacttatg ggaccagggg aacagcatat
      721 gcagaaatag acacagaggc agacaaattg tctgaacaca tttatgatga accatatgaa
      781 gaaaaggaga aggcagataa aaatgaggaa aaggaccatg ttagaaaggt aaagaaaata
      841 gtacaaagaa aagaaaatag tgaacataag agaaaagaga aggatcaaaa ggccttttta
      901 gccacagatt ggaacgatga tgacctgtcc cctgaggatt gggataattt ggaggaacaa
      961 gcggcacatt atcatgatga tgatgagttg atccttccag taaaaaggaa ggtggttaag
     1021 aagaaacctc tggcactcag aaggaaaccc ctgcctccgg taggttttgc aggagcaatg
     1081 gcagaggcca gggaaaaggg agatttgact tttacgtttc ctgtagtttt tatgggagaa
     1141 agtgatgatg atgatacacc tgtttgggaa ccgctgccat tgaaaacctt aaaagaacta
     1201 caatcggcag ttaggactat gggaccatct gctccataca ccttggaggt ggtagacatg
     1261 gtagctagtc agtggctcac cccgagtgat tggcaccaaa cagccagagc taccttatcc
     1321 cctggggatt atgttttatg gagaactgaa tatgaagaaa aaagtaaaga g
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