From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Rat peptidylglycine alpha-amidating monooxygenase (PAM) gene, exon
Date: 28 Dec 92 21:44:54 GMT
Distribution: bionet
Checksum: 08467 6

LOCUS       RATPAM12     1373 bp ds-DNA             ROD       01-DEC-1992
DEFINITION  Rat peptidylglycine alpha-amidating monooxygenase (PAM) gene, exon
            13.
ACCESSION   L01679
KEYWORDS    alternative splicing;
            peptidylglycine alpha-amidating monooxygenase.
SEGMENT     12 of 26
SOURCE      Rattus norvegicus (strain Sprague-Dawley) (library: lambda Fix)
            male adult liver DNA.
  ORGANISM  Rattus norvegicus
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Rodentia; Myomorpha; Muridae; Murinae.
REFERENCE   1  (sites)
  AUTHORS   Stoffers,D.A., Ouafik,L. and Eipper,B.A.
  TITLE     Characterization of novel mRNAs encoding enzymes involved in
            peptide alpha-amidation
  JOURNAL   J. Biol. Chem. 266, 1701-1707 (1991)
  STANDARD  full automatic
REFERENCE   2  (sites)
  AUTHORS   Eipper,B.A., Green,C.B., Campbell,T.A., Stoffers,D.A.,
            Keutmann,H.T., Mains,R.E. and Ouafik,L.
  TITLE     Alternative splicing and endoproteolytic processing generate
            tissue-specific forms of peptidylglycine alpha-amidating
            monooxygenase (PAM)
  JOURNAL   J. Biol. Chem. 267, 4008-4015 (1992)
  STANDARD  full automatic
REFERENCE   3  (bases 1 to 1373)
  AUTHORS   Ouafik,L., Stoffers,D.A., Campbell,T.A., Johnson,R.C.,
            Bloomquist,B.T., Mains,R.E. and Eipper,B.A.
  TITLE     The multifunctional peptidylglycine alpha-amidating monooxygenase
            gene: Exon/intron organization of catalytic, processing and routing
            domains
  JOURNAL   Mol. Endocrinol. 6, 1571-1584 (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     exon            136..1253
                     /number=13
                     /gene="PAM"
     polyA_signal    425..430
                     /gene="PAM"
                     /note="putative"
     polyA_signal    649..655
                     /gene="PAM"
                     /note="putative"
     polyA_signal    1094..1099
                     /gene="PAM"
                     /note="putative"
     polyA_signal    1133..1138
                     /gene="PAM"
                     /note="putative"
     intron          order(L01678:290..387,1..135)
                     /number=12
                     /gene="PAM"
                     /note=".52 kb gap"
     mat_peptide     join(L01669:24..143,L01670:71..128,L01671:187..274,
                     L01672:89..174,L01673:162..245,L01674:77..125,
                     L01675:163..230,L01676:227..307,L01677:67..143,
                     L01678:186..289,136..151)
                     /EC_number="1.14.17.3"
                     /product="peptidylglycine alpha-amidating monooxygenase"
                     /gene="PAM"
                     /codon_start=1
     mRNA            join(L01668:114..518,L01669:23..143,L01670:71..128,
                     L01671:187..274,L01672:89..174,L01673:162..245,
                     L01674:77..125,L01675:163..230,L01676:227..307,
                     L01677:67..143,L01678:186..289,136..1253)
                     /product="peptidylglycine alpha-amidating monooxygenase"
                     /gene="PAM"
                     /partial
                     /note="minor alternative mRNA"
     CDS             join(L01668:415..518,L01669:23..143,L01670:71..128,
                     L01671:187..274,L01672:89..174,L01673:162..245,
                     L01674:77..125,L01675:163..230,L01676:227..307,
                     L01677:67..143,L01678:186..289,136..154)
                     /EC_number="1.14.17.3"
                     /gene="PAM"
                     /note="alternatively spliced inactive PAM5 protein"
                     /product="peptidylglycine alpha-amidating monooxygenase"
                     /codon_start=1
                     /translation="MAGRARSGLLLLLLGLLALQSSCLAFRSPLSVFKRFKETTRSFS
                     NECLGTIGPVTPLDASDFALDIRMPGVTPKESDTYFCMSMRLPVDEEAFVIDFKPRAS
                     MDTVHHMLLFGCNMPSSTGSYWFCDEGTCTDKANILYAWARNAPPTRLPKGVGFRVGG
                     ETGSKYFVLQVHYGDISAFRDNHKDCSGVSVHLTRVPQPLIAGMYLMMSVDTVIPPGE
                     KVVNADISCQYKMYPMHVFAYRVHTHHLGKVVSGYRVRNGQWTLIGRQNPQLPQAFYP
                     VEHPVDVTFGDILAARCVFTGEGRTEATHIGFKDTF"
BASE COUNT      448 a    232 c    289 g    404 t
ORIGIN      
        1 cccaaacata ctgacaatct ctaaggcatg aaggactctt cttaaaaata aattagaaac
       61 ttaaaatgaa aggcaagctt gcatgggctt tctgtgagaa aagaatggag gtgcctcctt
      121 tccatgttga cctagattca aagatacttt ctgagtgcaa ttggaagctt gggagaagat
      181 acagaaaagt gaaggtgata cagaaaaaaa gagttttcag aagagtcgtg actacgaact
      241 aggaaccaca ctggagagga gagaggaagg acaggagcat ttgatggagt tttcatagat
      301 gatgggtttt cgtagaagat ggctctccgt cttaccttaa actatcaatt tgtatcaaag
      361 aagaagcttt caagtagcat tcctagtgta tttgttaaat ttttggagaa ggctggagaa
      421 aatcaataaa cgtattgact ttcataaagg aagatagacg atatatacac aatcacactg
      481 cgtagaggga caggccgtga cctccgtgac agtctgtgac gcttggggtt ccacggggca
      541 gagagtattg atcagctgcc ctctgttcgg tgctcttgtg tggccacagg tagttaaacg
      601 ccagaggtta ttccgttaac cttttcagaa agtaaagaat gtagttcaaa ataaaccctt
      661 tcagagagct gcatgtattg atttacaagc tcaagtggga ggcagtagag tattttaacc
      721 ctccagggcc ttcacccttt ggataagggg gcatattaca cccttggcca gactgtatct
      781 gtggaggcat taacggcttg ttacagtgcc agaatgagaa accggagtgg cccccagaga
      841 tttcttccgg gttcatgagt ctagaataat tccagccttt ttaaaattat acattttgct
      901 ttaaaatgat accctaaaat gaggatatta ctctattctt tcgtaactct ctggtgagat
      961 aaagtaaaca ctatttttaa tacagaggag atagaaaggg attatggcaa attaagttgt
     1021 tggatgagtc tgaattttga atgtttaatt tatactactg tataaaacga ctatagtttt
     1081 tttccagaag aataataaac taataaggca tgtttgaaga aatctaatgg tgaataaaat
     1141 ttttattatg atgcatatta tatatgcata taatgtttaa tgtgcctttt aaatttggaa
     1201 aaaatactgt ctacagtgtt tctgtgtttt gtacaatagc aggtgtagtc tccacaatac
     1261 cataagtacc cagtgaaaat ataaacagta aagaaaaaaa gatttcttag taaattattg
     1321 tctgtaaatc tccttctttt ctctcctccc acccgcacac acacacacac aca
//