From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human germ line n-myc gene.
Date: 5 Nov 92 23:11:56 GMT
Distribution: bionet
Checksum: 27436 15

LOCUS       HUMNMYCA     8762 bp ds-DNA             PRI       15-JUN-1989
DEFINITION  Human germ line n-myc gene.
ACCESSION   Y00664
KEYWORDS    amplification; c-myc oncogene; n-myc cellular oncogene;
            proto-oncogene.
SOURCE      human (Homo sapiens).
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 8762)
  AUTHORS   Ibson,J.M.
  JOURNAL   Unpublished (1988) U of CA at SF,3rd and Parn.,San Fran.,CA 94143
  STANDARD  full automatic
REFERENCE   2  (bases 1 to 8762)
  AUTHORS   Ibson,J.M. and Rabbitts,P.H.
  TITLE     Sequence of a germ-line N-myc gene and amplification as a mechanism
            of activation
  JOURNAL   Oncogene (1988) In press
  STANDARD  full automatic
COMMENT     *source: cell line=T-cell line SUP-T1; library=lambda gt11;
            *source: clone=lambda SUP-T1.4; the sequence represents a
            non-activated N-myc gene; see X03293-95 and M13241 for activated
            amplyfied N-myc sequences

              EMBL features not translated to GenBank features:
              key        from     to       description

              SITE       1395   1399       CTF binding site
              SITE       1696   1700       CTF binding site
              SITE       1730   1735       GC-box
              SITE       1799   2004       GC-box
              PRM        1828   1836       pot. TATA-box
              TRANSCR       ?   8295       primary transcript
              MSG           ?   2360       exon 1

              PRM        2152   2157       pot. alt. TATA-box
              IVS        2361   3254       intron I
              MSG        3255   4161       exon 2
              RBS        3390   3395       pot. ribosome binding site

              IVS        4162   6797       intron II
              MSG        6798   8295       exon 3

              SITE       8289   8294       polyA signal
              POLYA      8295   8295       polyA site.
FEATURES             Location/Qualifiers
     CDS             join(3396..4161,6798..7402)
                     /gene="MYCN"
                     /product="N-myc protein"
                     /codon_start=1
                     /translation="MPGMICKNPDLEFDSLQPCFYPDEDDFYFGGPDSTPPGEDIWKK
                     FELLPTPPLSPSRGFAEHSSEPPSWVTEMLLENELWGSPAEEDAFGLGGLGGLTPNPV
                     ILQDCMWSGFSAREKLERAVSEKLQHGRGPPTAGSTAQSPGAGAASPAGRGHGGAAGA
                     GRAGAALPAELAHPAAECVDPAVVFPFPVNKREPAPVPAAPASAPAAGPAVASGAGIA
                     APAGAPGVAPPRPGGRQTSGGDHKALSTSGEDTLSDSDDEDDEEEDEEEEIDVVTVEK
                     RRSSSNTKAVTTFTITVRPKNAALGPGRAQSSELILKRCLPIHQQHNYAAPSPYVESE
                     DAPPQKKIKSEASPRPLKSVVPPKAKSLSPRNSDSEDSERRRNHNILERQRRNDLRSS
                     FLTLRDHVPELVKNEKAAKVVILKKATEYVHSLQAEEHQLLLEKEKLQARQQQLLKKI
                     EHARTC"
     CDS             1894..2154
                     /product="open reading frame 1"
                     /codon_start=1
                     /translation="MRGAPGNCVGAEQALARRKRAQTVAIRGHPRPPGPPGDTRAESP
                     PDPLQSAGGKEQGLQTARRPGKRRAPGQGKPWTGLRRAHRAP"
BASE COUNT     1977 a   2256 c   2408 g   2121 t
ORIGIN      
        1 ttcagcagga gggcttggtg gcgattaggg ggctagggaa aagcagaagt ggggctgtaa
       61 aaggcttttg caaagatgaa ggcttttaca tttgtgaggg aaataacaat aataaaggtg
      121 cttggaaggg ctgctcatcc gcattcatgg gcctcttcct agaactctgg gaatctttct
      181 ccctagagct ctgtaggcgt ccagggtcag ggctggaaag gccttcacaa atgctaatct
      241 gtgagcattt tatgggttgc cccctgaccc tcagcatcct cctccttaac tagatccaat
      301 ggtgaggtga agaggtgggg gcgatggaga atggcatttc cagtttggag aaaaaagtag
      361 ggagaggcgg ggaagccttc ccaccccaat cccagggcca ggttcccctc tctccaaaca
      421 agcctaagga atgtttttca aggcaaaatc ccacctcact gtgtatttgg ttgtgaaggg
      481 ggctgtccag gagatcaggg ttgtttttgg agagtttgga gggcaggggc aataatgtct
      541 gaccccctgc aaacaaatac acctgtaaga aaagctagac cagagtgaag taaagccagg
      601 atttgatggt ggcgggaagg gaaggggcca atgctgctgc tggacagagg ggtgttattt
      661 tccccagaag aatagcatgt cgtggtcatc atcataataa tagctgacac aactattatg
      721 ggctggggac ttataagagc tttcataact ctcaggtagg aggcactacg aactccattt
      781 cacaattgag gaaactgagg cacaaagaaa cgaagtagtg acagcagctt tcctttttcc
      841 cttcaacagt ttggaatcaa gctgtttgag ccgaggctgg gtctcaggag gtgtggacag
      901 cacttccatc tcggtaaatc agattcgcct gcccataact ggggagaatg gtggctttga
      961 aaaggttaac ttgggagccc tggggacggc tgcagggaac gtacgcccat tccccctagg
     1021 aagcagagcc aggccgcctc ctcctccgca gtggtgagga cgcgtggaca gtcccgcggg
     1081 ggcggcggga gacctgcgga gtggccgcac tccaggtccg cgccaaggcc gggcagctcc
     1141 gctttctgct cagtctccgc gaggtgtcgc cttcggccga agaaaccacc gcggcgccac
     1201 cctcgtagct cgcacttatt tatttattta ttttcaaaca aggggggcgc ccctcttctt
     1261 tcaatttgaa actggaaaca tccagaggtc ttgttcctaa gggggcgcgt cctctccctg
     1321 ctattttgca ccttcggact agccttcttt cgtaattaca caggagcaac ctccctgcaa
     1381 ggccttgctc aacgttggcc tcgcgctcag ctgcacaaca cgcagtcaaa gcgggggctg
     1441 ggttagaagc atcggtctcc cctccccaac acacaccccc ggagccctcc gtaatttttt
     1501 tttcttttaa tgacaagcaa ttgccaggct cgcagggtgg gtgctgcatt gcaccgctcc
     1561 gcgcgcagct ggttctcaga gtgcagccgg tgcaagcccg ggggtccaaa agggcgggag
     1621 gagcacaccc tgggcttccc agctttgcag ccttctctct gcaaagaaaa gcaagtggct
     1681 tttggcgcga aagccttggc gcctcccctg atttttatgg aaatcaggag ggcggggtaa
     1741 agccgctttc ctctcctttc tccctccccc ttgtctgcgc cacagccccc ttctctcccc
     1801 gccccccggg tgtgtcagat ttttcagtta ataatatccc ccgagcttca aagcgcaggc
     1861 tgtgacagtc atctgtctgg acgcgctggg tggatgcggg gggctcctgg gaactgtgtt
     1921 ggagccgagc aagcgctagc caggcgcaag cgcgcacaga ctgtagccat ccgaggacac
     1981 ccccgccccc ccggcccacc cggagacacc cgcgcagaat cgcctccgga tcccctgcag
     2041 tcggcgggag gtaaggagca gggcttgcaa accgcccggc gcccagggaa gcgacgagcg
     2101 ccggggcaag gcaagccctg gacgggattg cgacgtgcgc accgggcgcc ctaatatgcc
     2161 cgggggactg tttctgcttc cgaaacaaaa ccatctctgg gttttcccag aaaagccagt
     2221 tccagccccg aaggcatcct ggctagagga gacccgccct aatccttttg cagcccttac
     2281 cggggggagt aatggcttct gcgaaaagaa attccctcgg ctctagaaga tctgtctgtg
     2341 tttgagctgt cggagagccg gtgcgtcccc accccaggct ggggttcttc tccaaagggt
     2401 gcccctggag gaagaagagg gggggattag gcagggcgag gccgccgcgg tcgcaatctg
     2461 ggtcacggct gctccagctt ggaggagagg cggctctccc ggcgaccctc ctcgcgcggg
     2521 cgcccctgcc attcccggga acaggggctc agcctctccc tccctggaag aggacgttgt
     2581 cgtgggtttg gaagagcagg ggtgggctta gagagcttcc aattaagcta ttggcaggag
     2641 tatccctgca gcgggtgaat gccgaggggc gtttgctcaa atttggggag gggaaggatt
     2701 tgtggatatg ggtgtctgtt gttggtctct gtctagagaa aggctttttt ttatttgcaa
     2761 agttttctaa atcccctgct atcatttgca ctcctgaggt tgcattttta caaagggggt
     2821 agaaggtact ccaaatacca ttcccggtag ctgggtcgga gagcctgggg cttcccctga
     2881 gcagccggcc ccacaccgct gcgagtgcgg ttgtctgcgt gctcgtgaga gctagaattc
     2941 tgcagccagg aacagccccc tcccccaggc agtgccttgt gtgaatgaaa tggcagtttc
     3001 caaagttgcg gagcctcgcc accaccccct gcatctgcat gccccctccc accccctgtc
     3061 gtagacagct tgtacacaaa aggagggcgg gagggaggga gcgagaggca caacttcctc
     3121 caccttcggg agcagtgggc agagtggggg gcttggaggg aagattgggg aacctggtta
     3181 gagggggcgc ccattgccta tcccctcggt ctgccccgtt tgcccaccct ctccggtgtg
     3241 tctgtcggtt gcagtgttgg aggtcggcgc cggcccccgc cttccgcgcc ccccacggga
     3301 aggaagcacc cccggtatta aaacgaacgg ggcggaaaga agccctcagt cgccggccgg
     3361 gaggcgagcc gatgccgagc tgctccacgt ccaccatgcc gggcatgatc tgcaagaacc
     3421 cagacctcga gtttgactcg ctacagccct gcttctaccc ggacgaagat gacttctact
     3481 tcggcggccc cgactcgacc cccccggggg aggacatctg gaagaagttt gagctgctgc
     3541 ccacgccccc gctgtcgccc agccgtggct tcgcggagca cagctccgag cccccgagct
     3601 gggtcacgga gatgctgctt gagaacgagc tgtggggcag cccggccgag gaggacgcgt
     3661 tcggcctggg gggactgggt ggcctcaccc ccaacccggt catcctccag gactgcatgt
     3721 ggagcggctt ctccgcccgc gagaagctgg agcgcgccgt gagcgagaag ctgcagcacg
     3781 gccgcgggcc gccaaccgcc ggttccaccg cccagtcccc gggagccggc gccgccagcc
     3841 ctgcgggtcg cgggcacggc ggggctgcgg gagccggccg cgccggggcc gccctgcccg
     3901 ccgagctcgc ccacccggcc gccgagtgcg tggatcccgc cgtggtcttc ccctttcccg
     3961 tgaacaagcg cgagccagcg cccgtgcccg cagccccggc cagtgccccg gcggcgggcc
     4021 ctgcggtcgc ctcgggggcg ggtattgccg ccccagccgg ggccccgggg gtcgcccctc
     4081 cgcgcccagg cggccgccag accagcggcg gcgaccacaa ggccctcagt acctccggag
     4141 aggacaccct gagcgattca ggtaaagacc gaactcgggt ccggctgcct ccctggggca
     4201 ctggaccccg ggtcgcgtcc cctttgttag tgctcgtatg tcttggcctg gggagcattt
     4261 tggaggcagt gctaggggca gagaggtcct gtttccccca agtctctcct cggggtaaag
     4321 agaaggggct gagagaatgc cgttgcaaaa ggggtgctct ccaattctcg ccttcactaa
     4381 agttccttcc accctctcct ggggagccct cctctaggcc atcacgggcc ctcacccggt
     4441 cccccacctc tcttttgcag cgcagtctga ggaataaaat tggagaaagt tggtggctaa
     4501 accgggtggg ggtttagggg gttgctgggt gcactgcctg gacagaaacc tgttagcgca
     4561 ggggtgaaag ggactctctg gcccaggtca ggggagggaa agacatcccc gagaagattc
     4621 aagggctgtg caaagccctg tttaaggcgc aggaacttat aggagggttg caacagatgg
     4681 ctagagccga ttttctattc tttttctttt tttttttttt ttttcaaatg tcggtacctt
     4741 tcccttcccc catcctcggt gggtggtggg ctatttgctc ctggtgcgtg gccagcaggc
     4801 ggcgatatgc gaggccagca ggcgggcccg ggatctgaaa ggctgggggt ggtgggggca
     4861 ccctccctcc ctccattcag cagctggctg caagtgcaac agcagttgtg tacattctca
     4921 gggggcctcc tctttccagt gtgcagtgga aagtggctgt agttttgtct tccagcctga
     4981 attccaggcc taatttgaga tgtgagttgt atctgtaacc cagtgccctt gaaggtgagg
     5041 gcaggcactc agcagcctct ccaggaaggc tcacatcctg ggaggactca ctgattagtt
     5101 ctattgtgtt catttgtctg tgtcttaagc tgaagggaag agttaaaacc aagcctttcc
     5161 ctgggggtct ggatgaacag aactcaaccc aaagagtggc attgccttgt ccttggagca
     5221 gggagctggg accccccttg gactttgaaa accagtgttt tcagaatgca ggtggataac
     5281 aagcctaaat ttacttctgg gctgaggaga gatctttgag gctcctggaa ggaaacttgg
     5341 tgataagcct ccagtttgaa acggctctgt ccctttaatg tctgtgcctt gacagctttt
     5401 ggtgaggaag cacttccttc caacagctgt cttcttggca gaaaaccaaa aattggctta
     5461 aagggaccca cagactggaa cagcctcaca tttcggcttt agaacaaatc ccacaattgt
     5521 tcagctttcc ggtccccttc agatcaagca gaagatatgt tttgattttc atgcttgtat
     5581 tttaaacaat aattttctac cccagcgtgg tagtcaatga ggagagaggg gaagaatgcg
     5641 cacatgatgc tacacgtttc tgttgttgct gttattattg gtggctttga ggagagctgc
     5701 tcccatttgg gggtttatac caactgtgga ttatggcttt gtcattaaga tttgatcttt
     5761 gttaaatgaa aaactgttta ttgtataaaa ctcaggtttg tggacgaaaa gttgtttttt
     5821 ttcttcagtt aattaaattg ttcctcaagt ttgtttaagg acttaaaatc aaacacaacc
     5881 atgtgtaaac tgctaaatga ggctcctaaa atgagaggcc tcaactcttt aagtgtggag
     5941 ctagaaatgt aaataagtcc acagggcaga ctggtgatta tgataaaagc taccatttac
     6001 tgagcatctg tctactaggc tcagctctat gctaagtcta catgttatct gtcaaagtgg
     6061 tatcatcccc atttaatagc tgaggaaaca gaggcttaga aaggctgggt aacttgacca
     6121 gggtcatgca actagtctgc ggtggagcca ggattctgtc tgaccctaaa ggccaagttc
     6181 tttatattta tttctaccac ctgctaaagt cttgaatgga ggctgaaagc acagttgggg
     6241 tatggggaag aaaaatatat atacatacat atatgtatat gtatgtatgt atgtatgggg
     6301 ggttgttttg tttttgtttt tgataaggag ttttgctctt gttgcccagg ctggagtgca
     6361 gtggtatgat ctgggctcac tgcaacctcc gcctcccggg ttcaagtcat tctcctgcct
     6421 cagcctcccg agtagctggg attaccggag catgccacca cacccagcaa agttttgtat
     6481 ttttagtaga gacagggttt caccatgttg gccaggctga tcttgaactc ctcatctcag
     6541 gtgatctgcc cgcctccgct tcccaaagtg ctgggattac aggtgtgagt caccgcgtcc
     6601 ggcctacaga tatatttaat ttaaagagat ctaaaacaaa tacaaaactg tccacatcta
     6661 tgttgatgga cccataaaaa tagcagtctg ccagggtctg ccggaagaga cagataagca
     6721 tacatattaa catggatata tatgtgaatt tcattcaaat ggttctcaca tgagagtaac
     6781 tagcatcttt ctctcagatg atgaagatga tgaagaggaa gatgaagagg aagaaatcga
     6841 cgtggtcact gtggagaagc ggcgttcctc ctccaacacc aaggctgtca ccacattcac
     6901 catcactgtg cgtcccaaga acgcagccct gggtcccggg agggctcagt ccagcgagct
     6961 gatcctcaaa cgatgccttc ccatccacca gcagcacaac tatgccgccc cctctcccta
     7021 cgtggagagt gaggatgcac ccccacagaa gaagataaag agcgaggcgt ccccacgtcc
     7081 gctcaagagt gtcgtccccc caaaggctaa gagcttgagc ccccgaaact ctgactcgga
     7141 ggacagtgag cgtcgcagaa accacaacat cctggagcgc cagcgccgca acgaccttcg
     7201 gtccagcttt ctcacgctca gggaccacgt gccggagttg gtaaagaatg agaaggccgc
     7261 caaggtggtc attttgaaaa aggccactga gtatgtccac tccctccagg ccgaggagca
     7321 ccagcttttg ctggaaaagg aaaaattgca ggcaagacag cagcagttgc taaagaaaat
     7381 tgaacacgct cggacttgct agacgcttct caaaactgga cagtcactgc cactttgcac
     7441 attttgattt tttttttaaa caaacattgt gttgacatta agaatgttgg tttactttca
     7501 aatcggtccc ctgtcgagtt cggctctggg tgggcagtag gaccaccagt gtggggttct
     7561 gctgggacct tggagagcct gcatcccagg atgctgggtg gccctgcagc ctcctccacc
     7621 tcacctccat gacagcgcta aacgttggtg acggttggga gcctctgggg ctgttgaagt
     7681 caccttgtgt gttccaagtt tccaaacaac agaaagtcat tccttctttt taaaatggtg
     7741 cttaagttcc agcagatgcc acataagggg tttgccattt gatacccctg gggaacattt
     7801 ctgtaaatac cattgacaca tccgcctttt gtatacatcc tgggtaatga gaggtggctt
     7861 ttgcggccag tattagactg gaagttcata cctaagtact gtaataatac ctcaatgttt
     7921 gaggagcatg ttttgtatac aaatatattg ttaatctctg ttatgtactg tactaattct
     7981 tacactgcct gtatacttta gtatgacgct gatacataac taaatttgat acttatattt
     8041 tcgtatgaaa atgagttgtg aaagttttga gtagatatta ctttatcact ttttgaacta
     8101 agaaactttt gtaaagaaat ttactatata tatatgcctt tttcctagcc tgtttcttcc
     8161 tgttaatgta tttgttcatg tttggtgcat agaactgggt aaatgcaaag ttctgtgttt
     8221 aatttcttca aaatgtatat atttagtgct gcatcttata gcactttgaa atacctcatg
     8281 tttatgaaaa taaatagctt aaaattaaat gatgcaactc aaccttttcc ttaatggcat
     8341 tacactctgt cccttaagga gcaaccataa ataatccata acctatagag ggaatttggt
     8401 ttccgtaaat agcccttttt gcacctgtac aatcctggtt ggggggcatg agtcattgtc
     8461 cccacttaag gtggagggaa ctgagtttgg ggaaattaag gcagttctcc aagattatgc
     8521 agaatagaga tgttattagc gactattgtg tgcattgtag caatggcatt tgataattta
     8581 cagagcactt ctgtatactg tggctcctta gtggaattaa gctgagacct cagatcagtc
     8641 cctttaaaag aaaagtaaaa atagccacag ggttgtttaa ctcgcttgta ttgggctttg
     8701 gtagtattcg tcccattggc agacagtctt ctattttaag gtagagcata gtttgtctcc
     8761 aa
//