From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Medicago sativa isocitrate dehydrogenase mRNA, 3' end cds.
Date: 31 Dec 92 01:33:29 GMT
Distribution: bionet
Checksum: 01269 4

LOCUS       ALFISDHYA    1577 bp ss-mRNA            PLN       17-DEC-1992
DEFINITION  Medicago sativa isocitrate dehydrogenase mRNA, 3' end cds.
ACCESSION   M93672 M93982
KEYWORDS    isocitrate dehydrogenase.
SOURCE      Medicago sativa callus cell suspension culture cDNA to mRNA.
  ORGANISM  Medicago sativa
            Eukaryota; Plantae; Embryobionta; Magnoliophyta; Magnoliopsida;
            Rosidae; Fabales; Fabaceae.
REFERENCE   1  (bases 1 to 1577)
  AUTHORS   Shorrosh,B.S. and Dixon,R.A.
  TITLE     Molecular characterization and expression of an isocitrate
            dehydrogenase from alfalfa (Medicago sativa L.)
  JOURNAL   Plant Mol. Biol. 20, 801-807 (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     sig_peptide     <1..65
                     /codon_start=3
     mat_peptide     66..1301
                     /product="isocitrate dehydrogenase"
                     /codon_start=1
     polyA_signal    1569..1574
     polyA_site      1577
     CDS             <1..1304
                     /product="isocitrate dehydrogenase"
                     /codon_start=3
                     /translation="QFSPNLSFSAFFPIITFTTATMGFQKIKVANPIVEMDGDEMTRI
                     IWKYIKDKLIFPFVELDIKYFDLGLPYRDETNDKVTVESAEATLKYNVAIKCATITPD
                     EARVKEFGLKSMWRSPNGTIRNILNGTVFREPIICKNIPRLIPGWTKPICIGRHAFGD
                     QYRATDSVIKGPGKLKLVFVPEGQGETTDLEVYNFTGEGGVALAMYNTDESIRSFAEA
                     SMAVALEKKWPLYLSTKNTILKKYDGRFKDIFQEVYEAGWKSKYEAAGIWYEHRLIDD
                     MVAYALKSEGGYVWACKNYDGDVQSDFLAQGFGSLGLMTSVLVCPDGKTIEAEAAHGT
                     VTRHFRVHQKGGETSTNSIASIFAWTRGLAHRAKLDDNATLLDFTEKLEAACIGVVES
                     GKMTKDLALILHGSKLSREHYLNTEEFIDAVAAELKTKISA"
BASE COUNT      450 a    289 c    383 g    455 t
ORIGIN      
        1 ctcagttctc acctaacctc tctttctccg cattttttcc gatcatcacc ttcaccaccg
       61 ccaccatggg attccagaaa atcaaagtcg ccaatcccat cgttgagatg gacggagatg
      121 aaatgacccg tattatctgg aagtatatca aggataagct catttttccc ttcgtggagt
      181 tggacatcaa gtactttgac ctaggccttc cttatcgtga tgagaccaat gataaagtta
      241 cagttgagag tgcagaagca accctcaagt ataatgtagc aatcaagtgt gcaactatca
      301 ctccagatga agctcgtgtc aaggagtttg gcctgaagag tatgtggagg agtccaaatg
      361 gaacaatcag aaacattttg aatggaactg tcttcagaga accaattatt tgcaaaaaca
      421 tccctcgcct tattcctggt tggacaaagc caatatgtat tggaaggcat gcttttggtg
      481 atcaatatcg ggccactgac tcggtgatta aagggccagg aaagctgaag ctggtttttg
      541 ttccggaagg ccagggtgag accaccgatt tggaggttta taacttcacc ggtgaaggag
      601 gtgttgcatt ggccatgtac aacactgacg agtctattcg ttcttttgct gaggcgtcta
      661 tggctgttgc tttagagaaa aaatggccac tttatcttag caccaaaaat acaattctta
      721 agaaatatga tggaagattc aaggacatat ttcaagaagt ttatgaggca ggctggaaat
      781 caaagtatga ggctgctggt atatggtatg agcaccggct cattgatgat atggtggcat
      841 atgcactcaa gagtgaagga ggttatgtat gggcatgcaa aaactatgat ggagatgtcc
      901 agagtgattt cttagctcaa ggttttggat cattgggttt gatgacttct gtattggttt
      961 gccctgatgg gaagaccata gaagcagaag cagcccatgg gacagttacc cgtcatttca
     1021 gggttcacca aaagggtgga gaaaccagca caaatagcat agcatccatc tttgcttgga
     1081 caagaggtct agcccatagg gcaaaactgg atgacaatgc tacactcttg gatttcactg
     1141 aaaagctaga ggcagcttgt attggagttg tagagtctgg gaagatgacc aaggatttgg
     1201 cacttattct tcatggatcc aagctgtcta gggagcatta tttgaacaca gaggaattca
     1261 tcgatgctgt tgcagctgag cttaaaacaa agatctctgc gtagatttgt gatttgagat
     1321 gggagaaggg acaagattga aaggatcaat gcactttcaa aagaaataag aaaggggaac
     1381 gaagcattgg ccactgcgct cttttccttc atttgtctta tggatggatg ggaatgcaag
     1441 ggttgggtgt ttccccatat tgtttgactg ttttattgtt tgtaattttg aactaaatgc
     1501 cttagaagtt taaatggatt ttgctttctt tctgtcgatt ttaacttcgt atgcttgcat
     1561 cgtttcatta taaaagt
//