From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Klebsiella aerogenes nitrogen assimilation control protein (nac)
Date: 5 Sep 92 10:02:07 GMT
Distribution: bionet
Checksum: 46453     8

LOCUS       KPNNACP      2936 bp ds-DNA             BCT       05-SEP-1992
DEFINITION  Klebsiella aerogenes nitrogen assimilation control protein (nac)
            gene, complete cds and transfer RNA-Asn gene.
ACCESSION   L01114
KEYWORDS    nac gene; nitrogen assimilation control protein; transfer RNA-Asn.
SOURCE      Klebsiella aerogenes (individual_isolate KC1043, strain W70) DNA.
  ORGANISM  Klebsiella aerogenes
            Prokaryotae; Gram-negative facultatively anaerobic rods;
            Enterobacteriaceae.
REFERENCE   1  (bases 1 to 2936)
  AUTHORS   Bender,R.A. and Schwacha,A.
  TITLE     The nac (Nitrogen Assimilation Control) gene from Klebsiella
            aerogenes
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     CDS             <1..272
                     /note="unusual codon usage"
                     /note="ORF X"
                     /codon_start=3
                     /translation="IPVSRPCGPMPAICCSKPISGGFTITTMTRGRMSGWSWLSAPGW
                     RNICNERSLSPSVRNWFRLRRAHNISDITFYEVLREIDLKEESLR"
     tRNA            complement(366..441)
                     /product="transfer RNA-Asn"
                     /gene="tRNA-Asn"
     protein_bind    566..582
                     /function="enhancer; transcription activation"
                     /bound_moiety="NtrC (NRI)"
                     /standard_name="NtrC-site"
                     /note="strong NtrC-binding (NRI-binding) site"
                     /evidence=EXPERIMENTAL
     protein_bind    584..602
                     /function="weak enhancer; transcriptional activation"
                     /bound_moiety="NtrC (NRI)"
                     /standard_name="NtrC-site"
                     /note="weak NtrC-binding (NRI-binding) site"
                     /evidence=EXPERIMENTAL
     promoter        699..715
                     /function="NTR-regulated transcription of nac gene"
                     /note="sigma54-dependent promoter"
                     /gene="nac"
                     /evidence=EXPERIMENTAL
     CDS             772..1689
                     /function="transcriptional regulator"
                     /product="nitrogen assimilation control protein"
                     /gene="nac"
                     /evidence=EXPERIMENTAL
                     /codon_start=1
                     /translation="MNLRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGEMD
                     QQLLIRTKRGVTPTEAGKILYTHARTILRQCEQAQLAVNNVGQTLRGQVSIGLAPGTA
                     ASAITMPLLQTVRNELPEVMVYLQESSGTALNDKLLAGQLDMAVLYERSPVAGIVSQP
                     LLKEDLYLVGTRDCPGQSVDLTAVAEMNLFLPRDYSAVRARVTEAFTLRRLSAKIIGE
                     IESITTLTAAIASGMGATVLPESAARSLCGAANGWMARISTPSMSLSLSLNMSARGSL
                     SPQAQAVKEILLSLVSRPSLENRELQLVS"
     CDS             1796..2746
                     /transl_except=(pos:1796..1798,aa:Met)
                     /note="Lys R member; very similar to CysB"
                     /note="ORF 2"
                     /evidence=EXPERIMENTAL
                     /codon_start=1
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLYTSQSGVSRHIRELEEEL
                     GIEIFIRRGKRLLGMTEPGKALLSIAERILNEASNVRRLADLFTNDASGVLTIATTHT
                     QARYSLPPVIKAFRELFSDVRVELVQGTPQEIEALLHNGGADIGIASERLSNDPTLAA
                     FPWFRWHHSLLVPKDHPLTQVSPLTLEAIARWPLITYRQGITGRSRIDEAFNRKGLMP
                     DIVLSAQDSDVIKTYVELGLGVGLVAEQSGDAREADTFTRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFIELCNAGLSLDEIKRQAMEPEEVAIDYQI"
     CDS             2825..>2936
                     /note="ORF 3"
                     /evidence=EXPERIMENTAL
                     /codon_start=1
                     /translation="MTMRRVKLLCTALMLLASHGALAVSYPLPPEGSRLVG"
BASE COUNT      645 a    800 c    892 g    599 t
ORIGIN
        1 ggatcccagt gagcaggccc tgcgggccga tgccggcaat atgctgctcg aaacctatca
       61 gcggcggctt cactataacg acaatgacga ggggcaggat gtcgggctgg agctggctaa
      121 gcgcgccagg ctggagaaat atatgcaacg agaggtcatt atcgcccagc gtcaggaact
      181 ggttccggct gcgccgcgcc cataacatca gcgatatcac tttttatgag gtgctgcggg
      241 agatagatct gaaggaagag agtttacgct aaagccgcaa tcgccggagg gtggccattc
      301 gccttccggc agaagtaccg gcgctgaaaa gcaaaaagcc tgctcgaaag caggcttttt
      361 aaatttggct cctctgactg gactcgaacc agtgacatac ggattaacag tccgccgttc
      421 taccgactga actacagagg aatcgtgtga acggggcgca tattaacgat gccatccggc
      481 cttgtcaaag cctgattcaa agaattgcgt tcgactgctg acaatttcag caatcgccat
      541 ccatgcgctg tttttttcac catgttgcac cgttgtagtg cgaatgtacc cctaatgggg
      601 cataccacaa tctgcttacc gggcaaataa aaaccgaatt gctatcacta tcccttataa
      661 cactgcctgc ggcgggcgac agccgaggaa atgcaaagct ggcaagcaaa ttgcaaaagc
      721 cagtgcatca tactcccagc gttgacgctg gcgttcctta caggaggcaa aatgaactta
      781 agacgactga aatattttgt gaaaatcgtc gatatcggca gtctgaccca ggccgctgaa
      841 gtcttgcata tcgcccaacc ggcccttagc caacaggtcg cgaccctgga aggtgaaatg
      901 gaccagcagt tgctgatacg caccaagcga ggggtcacgc caacggaagc cggtaaaatt
      961 ctctacaccc atgcgcgcac tatcctgcgc cagtgcgagc aggcgcagct ggcggtcaat
     1021 aacgtcggcc agacgctgcg tggtcaggtg tcgatcggcc tggcgccggg caccgcggcc
     1081 tccgccatta ccatgccgtt gctgcaaacg gtacgcaatg agttgcccga ggtgatggtc
     1141 tatctgcagg agagcagcgg cacagcgctg aatgacaagc tgctggcggg ccagctggat
     1201 atggcggtgc tctatgagcg ttcgccggtg gcaggcatcg tcagccagcc gctgctgaaa
     1261 gaagaccttt acctggtcgg cacccgcgac tgcccggggc agagcgtgga tctcacggcg
     1321 gtggcggaga tgaacctgtt cctgccgcgc gattacagcg cagtgcgggc gcgggtcact
     1381 gaggcattca ccctgcgtcg tctgtcggcg aaaattatcg gcgagatcga atccattacc
     1441 accctgaccg cggccatcgc cagcggcatg ggggcgacgg tgttaccgga atccgctgcc
     1501 cgttccctgt gcggggcggc gaacggctgg atggcgcgga tcagcacgcc ttcaatgagc
     1561 ctgtcgctgt cgctgaacat gtcggctcgc ggcagtctgt cgccgcaggc gcaggcggtg
     1621 aaagagatcc tgctgtctct ggtgagccgc ccatcgctgg aaaaccgtga actgcagctg
     1681 gtcagttagg gtatattctc ttaaggaata agttgctggt ttttattatt tgttacctgg
     1741 gacaacagac tttaacaata gcagatacgt taagtccatt ggcccggagg aaagggtgaa
     1801 cttccagcag ctgaaaatta tccgtgaggc ggcccgtcag gattacaacc tgacggaagt
     1861 cgcgaatatg ctctatacct cgcagtcagg cgtcagtcgc catatccggg aactggaaga
     1921 ggagctgggg attgagatct ttatccgccg cggcaaacgc ctgttaggca tgaccgagcc
     1981 gggtaaagcg ctgttatcga ttgccgagcg gatcctcaat gaagccagta acgttcggcg
     2041 gctggcggat ctcttcacta acgatgcgtc aggcgtctta accatcgcca ccacccatac
     2101 tcaggcgcgc tacagcctgc cgccggtcat taaagctttc cgcgagctgt tctcagatgt
     2161 tcgcgtcgag ctggtgcagg gtaccccgca ggaaatcgaa gcgctgctgc ataacggtgg
     2221 agcggatatc ggcatcgcca gcgagcgcct gagcaacgat ccgacgctgg cggctttccc
     2281 ctggttccgc tggcatcaca gcctgctggt gccgaaggat catcctttga cgcaggtttc
     2341 tcccctgacg ctggaggcga tcgcccgctg gccgttaatc acctaccgcc aggggatcac
     2401 tggccgttcg cgcattgatg aggcgttcaa ccgcaagggg ttgatgccgg atatcgtgct
     2461 gagcgcgcag gattccgatg tgatcaagac ctacgtggag ctggggctgg gcgtcgggct
     2521 ggtcgccgag cagtccggtg atgcccggga agcggacacc tttacgcgtc ttgatacccg
     2581 ccatctgttc gatgccaata ccgtgtggtt gggactgaag cggggccagc tgcagcggaa
     2641 ttacgtttgg cgctttatcg aattgtgcaa tgccggactg tcgctggatg agattaagcg
     2701 tcaggcgatg gagccggaag aggtcgcaat cgactatcag atttaaggaa atgctggcca
     2761 gcgggtcggg gagtggaaat aacgctataa ttcacacacg tgagtcgcga ctgaggaagg
     2821 gaaaatgacg atgcggcgtg tgaaattact ctgtacggcg ctgatgctgc ttgccagcca
     2881 cggcgcgctg gccgtcagct atccgctgcc gccggagggg agccgtttgg tgggca
//