From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Bacillus subtilis RNA polymerase sigma factor gene and
Date: 16 Dec 92 23:23:36 GMT
Distribution: bionet
Checksum: 65353 5

LOCUS       BACRNAPSF    1800 bp ds-DNA             BCT       15-DEC-1992
DEFINITION  Bacillus subtilis RNA polymerase sigma factor gene and
            DD-carboxypeptidase gene, complete cds.
ACCESSION   M85047
KEYWORDS    DD-Carboxypeptidase; sigma factor; sporulation operon.
SOURCE      Bacillus subtilis spore DNA.
  ORGANISM  Bacillus subtilis
            Prokaryotae; Firmicutes; Endospore-forming rods and cocci;
            Bacillaceae.
REFERENCE   1  (bases 1 to 1800)
  AUTHORS   Wu,J.-J., Schuch,R. and Piggot,P.J.
  TITLE     Characterization of a Bacillus subtilis sproulation operon that
            includes genes for an RNA polymerase sigma factor and for a
            putative DD-carboxpeptidase
  JOURNAL   J. Bacteriol. 174, 4885-4892 (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     misc_RNA        378..405
                     /function="transcription terminator"
                     /standard_name="RNA polymerase sigma factor"
                     /note="putative"
     RBS             483..492
     sig_peptide     502..570
                     /codon_start=1
     mat_peptide     571..1668
                     /product="DD-carboxypeptidase"
                     /codon_start=1
                     /note="putative"
     CDS             502..1671
                     /note="putative"
                     /product="DD-carboxypeptidase"
                     /codon_start=1
                     /translation="MKRLLSTLLIGIMLLTFAPSAFAKQDGKRTSELAHEAKSAVLIE
                     RDTGKVLYNKNSNERLAPASMTKIMTMLLIMEALDKGKIKMSDKVRTSEHAASMGGSQ
                     IFLEPGEEMTVKEMLKGIAIASGNDASVAMAEFISGSEEEFVKKMNKKAKELGLKNTS
                     FKNPTGLTEEGHYSSAYDMAIMAKELLKYESITKFTGTYEDYLRENTDKKFWLVNTNR
                     LIKFYPGVDGVKTGYTGEAKYCLTASAKKGNMRAIAVVFGASTPKERNAQVTKMLDFA
                     FSQYETHPLYKRNQTVAKVKVKKGKQKFIELTTSEPISILTKKGEDMNDVKKEIKMKD
                     NISAPIQKGQELGTLVLKKDGEVLAESPVAAKEDMKKAGFISFLKRTMGDWTKFK"
BASE COUNT      614 a    338 c    418 g    430 t
ORIGIN      
        1 cggtacccgg ggatctgtcc agcctggctg aaaagattgc gaaagacctt aatatcccaa
       61 ttcaaaaagg cgtgtacact gatgtgacag gaccttctta cgaaacaccg gcagaagtcc
      121 gtttcttaag aacgatgggc tctgatgcag tcggcatgtc tactgttccg gaagtcattg
      181 tagcgaatca tgcgggaatg cgggttcttg ccatttcctg catctctaac gcggcagccg
      241 gaattctgga tcagccttta agtcacgatg aagttatgga agtgaccgaa aaagtaaaag
      301 ctggattctt aaagcttgtt aaagcgatcg tcgctcagta cgaataaata tgaatcaatg
      361 caggaccaaa agacagctgt gtctgatatc acacagctgt ctttttttat gcccaaaatt
      421 cgaaacattt tccatcagtg gcgtataaaa ccatcacgct tggaaaaaat aaaaaggatt
      481 atgaaatgga gggcttttga gatgaaacgt cttttatcca ctttgttgat tggtataatg
      541 ctgcttacat ttgcaccgtc tgcatttgca aaacaagacg gaaaacgtac atcggagctt
      601 gctcatgaag cgaagtctgc ggtgctgata gaacgtgaca cgggaaaagt gctttacaac
      661 aagaacagca atgagagact ggcgcctgca agcatgacga aaattatgac gatgcttttg
      721 attatggaag ctttagataa aggcaaaatc aaaatgagtg ataaggtccg tacaagcgag
      781 catgcggcgt caatgggcgg ctcacagata ttccttgagc ccggcgaaga aatgactgtc
      841 aaagaaatgc tgaaaggcat cgcaatcgct tcgggaaatg acgcttccgt cgccatggct
      901 gaatttattt ccggctctga agaagaattt gtgaagaaaa tgaataaaaa agcaaaagag
      961 ctgggattga aaaatacatc ctttaaaaac ccaacaggac tgaccgagga aggacactac
     1021 agctctgctt atgacatggc aatcatggct aaggaattat tgaaatacga atcaattacg
     1081 aagtttaccg gcacgtatga agattatctg cgtgaaaata cagataaaaa gttttggctt
     1141 gtaaatacaa atcgccttat caaattttat cctggtgtag acggcgtaaa aacaggctat
     1201 acaggcgaag cgaaatattg tctgactgct tcggctaaaa aaggaaacat gcgggccata
     1261 gcggttgtat tcggagcgag cacgcctaaa gaaagaaacg cgcaagtgac aaaaatgctt
     1321 gacttcgcct ttagccaata tgaaacgcat cctttatata aacgaaatca aacagtagca
     1381 aaagtaaagg tcaaaaaagg gaaacaaaaa tttatcgaac tcactacatc tgagccgatt
     1441 tcaatattga cgaaaaaagg cgaggatatg aacgatgtga aaaaagaaat caagatgaag
     1501 gacaatatta gtgctccgat tcaaaaaggc caagagcttg gcactcttgt tctgaaaaag
     1561 gatggagaag tactcgctga aagtcctgtt gctgcaaaag aagatatgaa gaaagccggg
     1621 tttatctcat tcttaaagcg gacgatggga gactggacaa aatttaagta attatgccga
     1681 atgaccacta gttttgtcac ggtgaaggaa ttcattccgt cgaaatcgaa acactcatta
     1741 tccgatcata tcaaggagga atgagcatga gccttggaat tgacatgaat gtcaaagaat
//