From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: S.cerevisiae gene for acetyl-Coenzyme A synthetase
Date: 21 Jul 92 10:09:05 GMT
Distribution: bionet
Checksum: 05264     6

LOCUS       YSCACOASN    2492 bp ds-DNA             PLN       21-JUL-1992
DEFINITION  S.cerevisiae gene for acetyl-Coenzyme A synthetase
ACCESSION   X66425
KEYWORDS    acetyl-coenzyme A synthetase.
SOURCE      Saccharomyces cerevisiae DNA.
  ORGANISM  Saccharomyces cerevisiae
            Eukaryota; Fungi; Eumycota; Hemiascomycetes; Endomycetales;
            Saccharomycetaceae.
REFERENCE   1  (bases 1 to 2492)
  AUTHORS   De Virgilio,C., Buerckert,N., Barth,G., Neuhaus,J.M., Boller,T. and
            Wiemken,A.
  TITLE     Cloning and disruption of a gene required for growth on acetate but
            not on ethanol: the acetyl-coenzyme a synthetase gene of
            saccharomyces cerevisiae
  JOURNAL   Yeast (1992) In press
  STANDARD  full automatic
COMMENT     *source: strain=GRF88; *source: clone_library=YCp50 genomic DNA
            library; *source: chromosome=I;
            From EMBL    entry SCACOASN;  dated 12-JUN-1992.
FEATURES             Location/Qualifiers
     TATA_signal     25..30
                     /note="putative"
     TATA_signal     40..45
                     /note="putative"
     TATA_signal     95..100
                     /note="putative"
     TATA_signal     108..113
                     /note="putative"
     TATA_signal     126..131
                     /note="putative"
     misc_feature    162..164
                     /note="potential start (1)"
     CDS             162..2303
                     /EC_number="6.2.1.1"
                     /product="acetate--CoA ligase"
                     /codon_start=1
                     /translation="MSPSAVQSSKLEEQSSEIDKLKAKMSQSAATAQRKKEHEYEHLT
                     SVKIVPQRPISDRLQPAIATHYSPHLDGLQDYQRLHKESIEDPAKFFGSKATQFLNWS
                     KPFDKVFIPDPKTGRPSFQNNAWFLNGQLNACYNCVDRHALKTPNKKAIIFEGDEPGQ
                     GYSITYKELLEEVCQVAQVLTYSMGVRKGDTVAVYMPMVPEAIITLLAISRIGAIHSV
                     VFAGFSSNSLRDRINDGDSKVVITTDESNRGGKVIETKRIVDDALRETPGVRHVLVYR
                     KTNNPSVAFHAPRDLDWATEKKKYKTYYPCTPVDSEDPLFLLYTSGSTGAPKGVQHST
                     AGYLLGALLTMRYTFDTHQEDVFFTAGDIGWITGHTYVVYGPLLYGCATLVFEGTPAY
                     PNYSRYWDIIDEHKVTQFYVAPTALRLLKRAGDSYIENHSLKSLRCLGSVGEPIAAEV
                     WEWYSEKIGKNEIPIVDTYWQTESGSHLVTPLAGGVTPMKPGSASFPFFGIDAVVLDP
                     NTGEELNTSHAEGVLAVKAAWPSFARTIWKNHDRYLDTYLNPYPGYYFTGDGAAKDKD
                     GYIWILGRVDDVVNVSGHRLSTAEIEAAIIEDPIVAECAVVGFNDDLTGQAVAAFVVL
                     KNKSSWSTATDDELQDIKKHLVFTVRKDIGPFAAPKLIILVDDLPKTRSGKIMRRILR
                     KILAGESDQLGDVSTLSNPGIVRHLIDSVKL"
     misc_feature    234..236
                     /note="potential start (2)"
     polyA_signal    2247..2452
                     /note="putative"
     polyA_signal    2472..2477
                     /note="putative"
BASE COUNT      673 a    554 c    521 g    744 t
ORIGIN
        1 aaatagttct cccttcattt tgtatataaa ctgggcggct attctaagca tatttctccc
       61 ttaggttatc tggtagtacg ttatatcttg ttcttatatt ttctatctat aagcaaaacc
      121 aaacatatca aaactactag aaagacattg cccactgtgc tatgtcgccc tctgccgtac
      181 aatcatcaaa actagaagaa cagtcaagtg aaattgacaa gttgaaagca aaaatgtccc
      241 agtctgccgc cactgcgcag cgaaagaagg aacatgagta tgaacatttg acttcggtca
      301 agatcgtgcc acaacggccc atctcagata gactgcagcc cgcaattgct acccactatt
      361 ctccacactt ggacgggttg caggactatc agcgcttgca caaggagtct attgaagacc
      421 ctgctaagtt cttcggttct aaagctaccc aatttttaaa ctggtctaag ccattcgata
      481 aggtgttcat cccagaccct aaaacgggca ggccctcctt ccagaacaat gcatggttcc
      541 tcaacggcca attaaacgcc tgttacaact gtgttgacag acatgccttg aagactccta
      601 acaagaaagc cattattttc gaaggtgacg agcctggcca aggctattcc attacctaca
      661 aggaactact tgaagaagtt tgtcaagtgg cacaagtgct gacttactct atgggcgttc
      721 gcaagggcga tactgttgcc gtgtacatgc ctatggtccc agaagcaatc ataaccttgt
      781 tggccatttc ccgtatcggt gccattcact ccgtagtctt tgccgggttt tcttccaact
      841 ccttgagaga tcgtatcaac gatggggact ctaaagttgt catcactaca gatgaatcca
      901 acagaggtgg taaagtcatt gagactaaaa gaattgttga tgacgcgcta agagagaccc
      961 caggcgtgag acacgtcttg gtttatagaa agaccaacaa tccatctgtt gctttccatg
     1021 cccccagaga tttggattgg gcaacagaaa agaagaaata caagacctac tatccatgca
     1081 cacccgttga ttctgaggat ccattattct tgttgtatac gtctggttct actggtgccc
     1141 ccaagggtgt tcaacattct accgcaggtt acttgctggg agctttgttg accatgcgct
     1201 acacttttga cactcaccaa gaagacgttt tcttcacagc tggagacatt ggctggatta
     1261 caggccacac ttatgtggtt tatggtccct tactatatgg ttgtgccact ttggtctttg
     1321 aagggactcc tgcgtaccca aattactccc gttattggga tattattgat gaacacaaag
     1381 tcacccaatt ttatgttgcg ccaactgctt tgcgtttgtt gaaaagagct ggtgattcct
     1441 acatcgaaaa tcattcctta aaatctttgc gttgcttggg ttcggtcggt gagccaattg
     1501 ctgctgaagt ttgggagtgg tactctgaaa aaataggtaa aaatgaaatc cccattgtag
     1561 acacctactg gcaaacagaa tctggttcgc atctggtcac cccgctggct ggtggtgtta
     1621 caccaatgaa accgggttct gcctcattcc ccttcttcgg tattgatgca gttgttcttg
     1681 accctaacac tggtgaagaa cttaacacca gccacgcaga gggtgtcctt gccgtcaaag
     1741 ctgcatggcc atcatttgca agaactattt ggaaaaatca tgataggtat ctagacactt
     1801 atttgaaccc ttaccctggc tactatttca ctggtgatgg tgctgcaaag gataaggatg
     1861 gttatatctg gattttgggt cgtgtagacg atgtggtgaa cgtctctggt caccgtctgt
     1921 ctaccgctga aattgaggct gctattatcg aagatccaat tgtggccgag tgtgctgttg
     1981 tcggattcaa cgatgacttg actggtcaag cagttgctgc atttgtggtg ttgaaaaaca
     2041 aatctagttg gtccaccgca acagatgatg aattacaaga tatcaagaag catttggtct
     2101 ttactgttag aaaagacatc gggccatttg ccgcaccaaa attgatcatt ttagtggatg
     2161 acttgcccaa gacaagatcc ggcaaaatta tgagacgtat tttaagaaaa atcctagcag
     2221 gagaaagtga ccaactaggc gacgtttcta cattgtcaaa ccctggcatt gttagacatc
     2281 taattgattc ggtcaagttg taatgatgat ttctttcctt tttatattga cgactttttt
     2341 tttttcgtgt gtttttgttc tcttataacc gagctgctta cttattatta tttcaccttc
     2401 tctttttatt tatacttata attatttatt ctttacatac tgttacaaga aactcttttc
     2461 tacattaatt gcataaagtg tcaatcagca ca
//