From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Mouse type 1 glucose transporter (GLUT1) gene, 5' flanking region
Date: 21 Jul 92 10:07:08 GMT
Distribution: bionet
Checksum: 60757     5

LOCUS       MUSGLUT1     1565 bp ds-DNA             ROD       21-JUL-1992
DEFINITION  Mouse type 1 glucose transporter (GLUT1) gene, 5' flanking region
            and exon 1.
ACCESSION   D10229
KEYWORDS    glucose transporter protein; immediate early gene;
            membrane glycoprotein.
SOURCE      Mus musculus (strain Swiss albino) (cell line: 3T3-L1) (library:
            bacteriophage lambda EMBL4 mouse genomic library) DNA
  ORGANISM  Mus musculus
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Rodentia; Myomorpha; Muridae; Murinae.
REFERENCE   1  (bases 1 to 1565)
  AUTHORS   Murakami,T., Nishiyama,T., Shirotani,T., Shinohara,Y., Kan,M.,
            Ishii,K., Kanai,F., Nakazuru,S. and Ebina,Y.
  TITLE     Identification of two enhancer elements in the gene encoding the
            type 1 glucose transporter from the mouse which are responsive to
            serum, growth factor and oncogenes
  JOURNAL   J. Biol. Chem. (1992) In press
  STANDARD  full staff_review
COMMENT     Data kindly submitted in computer readable form by:
            Takashi Murakami
            Institute for Enzyme Research
            The University of Tokushima
            3-18-15 Kuramoto
            Tokushima 770
            Japan
FEATURES             Location/Qualifiers
     GC_signal       complement(1201..1206)
     protein_bind    1217..1224
                     /bound_moiety="transcription factor AP2"
                     /note="pot. AP2 binding site"
     GC_signal       complement(1226..1231)
     CAAT_signal     1258..1262
                     /note="pot. CCAAT box"
     TATA_signal     1271..1274
                     /note="pot. TATA box"
     mRNA            1305..>1565
                     /note="two major mRNA start sites are known."
                     /evidence=EXPERIMENTAL
     exon            1305..1473
                     /number=1
     mRNA            1307..>1565
                     /note="two major mRNA start sites are known."
                     /evidence=EXPERIMENTAL
     CDS             1456..1473
                     /partial
                     /note="the part in exon 1"
                     /product="type 1 glucose transporter protein"
                     /gene="GLUT1"
                     /codon_start=1
                     /translation="MDPSSK"
                     /partial
     intron          1474..>1565
                     /number=1
     misc_feature    1475..1481
                     /function="transcriptional control"
                     /note="pot. TRE (TPA responsive element)"
BASE COUNT      369 a    435 c    442 g    319 t
ORIGIN
        1 aagatggctg attggtgact tgttggctga gatataggca tgcagttcta taatgggggc
       61 gactgaattt gtgaactggt accctaagaa agggcctggg agaccaggct tgggccttgt
      121 tccagccctg catcatatgt attgaaggaa agaaacaggt ccagggttgg agaaagcaag
      181 agcagaggag gcaggtcttg cagtcagcag aagaaactcg gggggggggg ggcaaagtgg
      241 tgatcaggag agaggagaag gggaacccca gactcaccag ttccacatgc tttacttttc
      301 aggggtccat gctcaattag ggtgccctcc taggccagca cctggcacac agctcagctt
      361 cctgtctcta acagaaagtc atttctccca gatcccccct tcaggtgaac ctagatggga
      421 catggactgg agggaccaca gaggctattg actgtcctac tcaccactcc aggagctcac
      481 ctgtaattct catagcccag ggtttgataa caaatctcat tttatccagg agaaacccgc
      541 tgctcattgg ctgggagctt gcccaggacc agctagaggc ggtttatagg tgagctggtt
      601 caatgccaag gtgttctatg tccttaacca tttagacacc cagcctctct ggtcttcaca
      661 cttaggtgtc accataaagt cagaaatgga gggaggtggt ggttttacag cttagagaag
      721 tggtgggcat ggtgtctagg agcaaaagag gagcttggcc ttgggcacag gaacacggag
      781 caaggagaga gaattcagct tcggagccag acctcccgtg gtctctctaa tgaacttaat
      841 gccactttac acataacaca cgcggttgta aacaccatcc aaatactaac tcgtttaatc
      901 ttcctaataa cccaggaggt tgtactatta ttatgactag tctaccaaag ttatccggca
      961 gcctagtgag gaagccagag tcctagcgct ggcttacagc cccgcaccaa gcgctaccat
     1021 tttgctagtg gccattgaga ctcagagagg tgtgcaaacc agctcagagc cacacagcga
     1081 gcgcccccaa cgggtgcccc gcacaccctg ggactgcagg ttctagcatc cagagccaga
     1141 ctgtggtcag tagccctccg tccctcccca cctccccaca gaaccacgcc tctcagagtc
     1201 ccgccccgga cgtgctcccc aggccccgcc cccttcgggc ccacctacac cccagaacca
     1261 atggcggcgg tcctataaaa aggcagctcc gcgcgctctc ccccaagagc agaggcttgc
     1321 ttgtagagtg acgatctgag ctacggggtc ttaagtgcgt cagggcgtgg aggtctggcg
     1381 ggagacgcat agttacagcg cgtccgttct ccgtctcgca gccggcacag ctagagcttc
     1441 gagcgcagcg cggccatgga tcccagcagc aaggtgagta acggggctgg gagtcccggg
     1501 tagggatgtg ctgccgagcg cgggccgggg cgcctgtccg agtccccgtt cctcacgcag
     1561 ccgcc
//