HDTenEightyP/NetNews huggingface dataset:
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human polymorphic microsatellite DNA.
Date: 19 Aug 92 10:01:02 GMT
Distribution: bionet
Checksum: 61650     2

LOCUS       HUM8PLYSAT    312 bp ds-DNA             PRI       19-AUG-1992
DEFINITION  Human polymorphic microsatellite DNA.
ACCESSION   M99148
KEYWORDS    microsatellite polymorphism.
SOURCE      Homo sapiens DNA.
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 312)
  AUTHORS   Bowcock,A.M.Ph.D.., Osborne-Lawrence,S.L., Barnes,R.I.,
            Chakravarti,A., Washington,S. and Dunn,C.
  TITLE     A linkage map of human chromosome 13q containing microsatellite
            polymorphisms
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     satellite       1..312
                     /note="map=13q; awaiting approval /map qualifier"
BASE COUNT      100 a     43 c     69 g    100 t
ORIGIN
        1 acttccacag attcttttct actacaaaaa tagaaataag gaacattaga ctcttcacgt
       61 gagattttta ctttctaagg tgcgcatata ccatagagat aagttctatg tctgtatatg
      121 tacatatgcg tgtgtggtgg gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt aaggtggctg
      181 aggggttaat tttcatctaa caggccagtg ttgagatgac ttctgttttt ctctaacctc
      241 ttgaactaat gaatgcaaaa taaaacaaca aaaatcaaag aaactaagca atagagaaaa
      301 gaaagggatt ta
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human chromosome 4 sequence-tagged site STS4-55
Date: 21 Aug 92 10:11:03 GMT
Distribution: bionet
Checksum: 52361     3

LOCUS       HUM4STS55     645 bp ds-DNA             PRI       21-AUG-1992
DEFINITION  Human chromosome 4 sequence-tagged site STS4-55
ACCESSION   M95301
KEYWORDS    STS; human chromosome 4; sequence tagged site.
SOURCE      Homo sapiens, clone L4-1 from the C25 lambda library (Pritchard,
            Genomics 4, 408, 1989); vector - Lambda Dash II (Stratagene)
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 645)
  AUTHORS   Goold,R.D., diSibio,G., Dugaiczyk,A., Smith,K.A., Xu,H.,
            Magrane,G., Lang,D.B., Cox,D.R., Masters,S.B. and Myers,R.M.
  TITLE     Sequence-Tagged Sites for Human Chromosome 4
  JOURNAL   Unpublished (1992) see COMMENT for author address
  STANDARD  full automatic
COMMENT     PCR components: 25 ng of human genomic DNA, 40 pmol of each
            oligonucleotide, 200 micro-M dNTPs, 0.25 U Taq  polymerase (Cetus)
            in 10 micro-l of 50 mM KCl-10 mM  Tris-HCl, pH 8.6 (at room temp),
            1.5 mM MgCl-2, 5 mM NH-4Cl.  Initial denaturation at 94degC for 1.5
            min, then 30 cycles of  94degC for 15 sec, 62degC for 23 sec, and
            72degC for 30  sec, followed by a final extension at 72degC for 3.5
            min, using a Perkin-Elmer 9600 thermocycler. PCR-amplified product
            size 153 bp.
            Sequence submitted by:
            Human Genome Mapping Center
            Box 0925
            University of California San Francisco
            San Francisco, CA 94143-0925  USA
            Phone: (415) 502-1612   Fax: (415) 476-8391
            e-mail: hgmcprobes@cgl.ucsf.edu
FEATURES             Location/Qualifiers
     misc_feature    85..112
                     /label=Primer1
     misc_feature    complement(210..237)
                     /label=Primer2
BASE COUNT      171 a    118 c    133 g    187 t     36 others
ORIGIN
        1 ttgtaatacg actcactata gggcgaattg gagctccacc gcggtggcgg ccgctctaga
       61 actagtggat cccccgggct gcaggaattc taataaaata ctctgaaaac tcttataaga
      121 ggaataggaa gcaattggac ccctgaagaa aagtataaaa ttcttagctg tattctcagg
      181 agccaaatcc aaacaaaaat tttatcaggc actaaaaatg tttagaaaat catgtggaat
      241 tcaaggaagg tgatattttg cagacaaaac agaaatgatt tacctctgag tgtaggatta
      301 tgtaatttgt cttaactgag ttcataccaa ttagaaaagg aaccactcag ttaagggcag
      361 atatctctcc taaacaattt taaaaattag ttttatatta tactttattg gcaacccttt
      421 taaagtaggn ttttgngnta ctagggtagg agggtttcag ttccgntaac tgtttccntt
      481 gctgngccan ctcctaggnt gntgcatccc tttgcctggt tgnatctggc tttaaacntn
      541 gtttnagggc ntcaggctgc tctcncnggc atnntccgtg gganngttng gnaagttcnt
      601 tcccgtnggc ccnggtnccn tgnnnnnttt cccnnttttt tnntg
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human chromosome 4 sequence-tagged site STS4-177
Date: 21 Aug 92 10:07:21 GMT
Distribution: bionet
Checksum: 48790     3

LOCUS       HUM4STS177    437 bp ds-DNA             PRI       21-AUG-1992
DEFINITION  Human chromosome 4 sequence-tagged site STS4-177
ACCESSION   M95247
KEYWORDS    STS; human chromosome 4; sequence tagged site.
SOURCE      Homo sapiens, clone C4-109 from Los Alamos National Laboratory
            chromosome 4 cosmid library, plate 4-1-1R, pos 7E; vector SuperCos
            1 (Stratagene)
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 437)
  AUTHORS   Goold,R.D., diSibio,G., Dugaiczyk,A., Smith,K.A., Xu,H.,
            Magrane,G., Lang,D.B., Cox,D.R., Masters,S.B. and Myers,R.M.
  TITLE     Sequence-Tagged Sites for Human Chromosome 4
  JOURNAL   Unpublished (1992) see COMMENT for author address
  STANDARD  full automatic
COMMENT     PCR components: 25 ng of human genomic DNA, 10 pmol of each
            oligonucleotide, 200 micro-M dNTPs, 0.25 U Taq  polymerase (Cetus)
            in 10 micro-l of 50 mM KCl-20 mM  Tris-HCl, pH 8.3 (at room temp),
            2.5 mM MgCl-2.  Initial denaturation at 94degC for 1.5 min, then 30
            cycles of  94degC for 15 sec, 62degC for 23 sec, and 72degC for 30
            sec, followed by a final extension at 72degC for 3.5 min, using a
            Perkin-Elmer 9600 thermocycler. PCR-amplified product size 189 bp.
            Sequence submitted by:
            Human Genome Mapping Center
            Box 0925
            University of California San Francisco
            San Francisco, CA 94143-0925  USA
            Phone: (415) 502-1612   Fax: (415) 476-8391
            e-mail: hgmcprobes@cgl.ucsf.edu
FEATURES             Location/Qualifiers
     misc_feature    57..80
                     /label=Primer1
     misc_feature    complement(221..245)
                     /label=Primer2
BASE COUNT       98 a     84 c     98 g    135 t     22 others
ORIGIN
        1 cngtgccaat cagcaaactn agtacggtna tgctttgaag aacgtgacca ttttggttac
       61 agacctcaat gacaatgtcc caatgtttat atcacaaaac gcccttgctg cagacccatc
      121 agctgtgatt ggttccgttc tgacaacaat tatggctgct gacccagatg aaggtgctaa
      181 tggagaaata gagtatgaga tcatcaatgg ggacacagac accttcattg ttgatcgtta
      241 tagtggngac ctnngagtgg cttnagcgtt ggtgccttca cagttgatct ncaatntcat
      301 agttttcagc aacagacctt tgggctgnaa ggaggaaatt gntcnctgnn tttgtccctt
      361 cattctttca tggggctttn ttggtcctgn tttttcttat nccnnntatt taactttttt
      421 ttnngggggg ggggttt
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human chromosome 4 sequence-tagged site STS4-241
Date: 21 Aug 92 10:08:04 GMT
Distribution: bionet
Checksum: 06698     3

LOCUS       HUM4STS241    477 bp ds-DNA             PRI       21-AUG-1992
DEFINITION  Human chromosome 4 sequence-tagged site STS4-241
ACCESSION   L00755
KEYWORDS    STS; human chromosome 4; sequence tagged site.
SOURCE      Homo sapiens, clone p4-183 from a chromosome 4 plasmid library;
            vector pBluescript II KS + (Stratagene)
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 477)
  AUTHORS   Goold,R.D., diSibio,G., Dugaiczyk,A., Smith,K.A., Xu,H.,
            Magrane,G., Lang,D.B., Cox,D.R., Masters,S.B. and Myers,R.M.
  TITLE     Sequence-Tagged Sites for Human Chromosome 4
  JOURNAL   Unpublished (1992) see COMMENT for author address
  STANDARD  full automatic
COMMENT     PCR components: 25 ng of human genomic DNA, 10 pmol of each
            oligonucleotide, 200 micro-M dNTPs, 0.25 U Taq  polymerase (Cetus)
            in 10 micro-l of 50 mM KCl-20 mM  Tris-HCl, pH 8.3 (at room temp),
            2.5 mM MgCl-2.  Initial denaturation at 94degC for 1.5 min, then 30
            cycles of  94degC for 15 sec, 62degC for 23 sec, and 72degC for 30
            sec, followed by a final extension at 72degC for 3.5 min, using a
            Perkin-Elmer 9600 thermocycler. PCR-amplified product size 190 bp.
            Sequence submitted by:
            Human Genome Mapping Center
            Box 0925
            University of California San Francisco
            San Francisco, CA 94143-0925  USA
            Phone: (415) 502-1612   Fax: (415) 476-8391
            e-mail: hgmcprobes@cgl.ucsf.edu
FEATURES             Location/Qualifiers
     misc_feature    95..118
                     /label=Primer1
     misc_feature    complement(260..284)
                     /label=Primer2
BASE COUNT      134 a    100 c     90 g    150 t      3 others
ORIGIN
        1 tccctgcagc ccgggggtat ctgcaggaaa acgtcaattc cttaagcaga gaatataaaa
       61 ttttatatca tctggtccct acccgccatg tgggtctcat cctgcataat tcccttacat
      121 atgttgagac acagccatat tacatggcct gaatgtatta gacatataag gttttattca
      181 gttatctgtt acttatccta ctttctgaaa atcattgaga atgaaattct acttcttctg
      241 actgagctca aattttaccc actcatttaa gccttctttg atgtctaaag ccaaagtaag
      301 gtcctccata aatacactct tatatatata ctggatatgt tacaggcatg gtatctgcca
      361 tgcccagact taagtaggct ttggccagag nccatgggtc tggggatagc atgcactatt
      421 ctgtgcacag acagaagcca atgattagat tataatttng gatntttctg aggggag
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human beta-hexosaminidase gene, exon 11.
Date: 22 Aug 92 10:01:00 GMT
Distribution: bionet
Checksum: 56755     2

LOCUS       HUMHEXBIIA    175 bp ds-DNA             PRI       22-AUG-1992
DEFINITION  Human beta-hexosaminidase gene, exon 11.
ACCESSION   D10517 D90494
KEYWORDS    beta-hexosaminidase; juvenile Sandhoff disease; lysosomal enzyme.
SOURCE      Human (patient with juvenile Sandhoff disease) DNA.
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 175)
  AUTHORS   Proia,R.L.
  TITLE     Gene encoding the human beta-hexosaminidase beta chain: extensive
            homology of intron placement in the alpha- and beta-chain genes
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 85, 1883-1887 (1988)
  STANDARD  full staff_review
REFERENCE   2  (bases 1 to 175)
  AUTHORS   Wakamatsu,N., Kobayashi,H., Niyatake,T. and Tsuji,S.
  TITLE     A novel exon mutation in the human beta-hexosaminidase beta subunit
            gene affecte 3' splice site selection
  JOURNAL   J. Biol. Chem. 267, 2406-2413 (1992)
  STANDARD  full staff_review
COMMENT     Data kindly submitted in computer readable form by:
            Shoji Tsuji
            Department of Neurology
            Brain Research Institute
            Niigata University
            1 Asahimachi-dori
            Niigata 951
            Japan
            Phone:  025-223-6161 x5184
            Fax:    025-225-6458
FEATURES             Location/Qualifiers
     exon            1..175
                     /number=11
                     /product="beta-hexosaminidase"
     variation       8
                     /note="replace(8, 'c')"
BASE COUNT       47 a     34 c     43 g     51 t
ORIGIN
        1 cttgcgctgg gcacaatagt tgaagtatgg aaagacagcg catatcctga ggaactcagt
       61 agagtcacag catctggctt ccctgtaatc ctttctgctc cttggatctt agatttgatt
      121 agctatggac aagattggag gaaatactat aaagtggaac ctcttgattt tggcg
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Homo sapiens DNA-binding protein (ZNF) gene, partial cds.
Date: 21 Aug 92 10:11:46 GMT
Distribution: bionet
Checksum: 21863     2

LOCUS       HUMZNFC       219 bp ds-DNA             PRI       21-AUG-1992
DEFINITION  Homo sapiens DNA-binding protein (ZNF) gene, partial cds.
ACCESSION   M88359
KEYWORDS    DNA binding protein; zinc finger protein.
SOURCE      Homo sapiens Placenta DNA.
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 219)
  AUTHORS   Bray,P.L., Lichter,P., Thiesen,H.J., Ward,D.C. and Dawid,I.B.
  TITLE     Characterization and mapping of human genes encoding zinc finger
            proteins
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 88, 9563-9567 (1991)
  STANDARD  full automatic
REFERENCE   2  (bases 1 to 219)
  AUTHORS   Lichter,P., Bray,P., Ried,T., Dawid,I.B. and Ward,D.C.
  TITLE     Clustering of C2-H2 zinc finger motif sequences within telomeric
            and fragile site regions of human chromosomes
  JOURNAL   Genomics 13, 999-1007 (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     CDS             1..219
                     /standard_name="zinc finger protein"
                     /product="DNA binding protein"
                     /gene="ZNF"
                     /note="putative"
                     /codon_start=1
                     /translation="IHTGEKPHKCNECGKSFCRLSHLIQHQRTHSGEKPYECEECGKS
                     FSRSSHLAQHQRTHTGEKPYECNECGRGF"
                     /partial
BASE COUNT       66 a     54 c     56 g     43 t
ORIGIN
        1 atccacactg gagagaagcc tcacaaatgt aatgagtgtg gaaaaagttt ctgccgtctc
       61 tctcacctaa tccaacacca aaggacccac agtggtgaga aaccctatga gtgtgaggag
      121 tgtgggaaaa gcttcagccg gagctctcat ctagctcagc accagaggac ccacacgggt
      181 gagaaacctt atgaatgtaa cgaatgtggc cgaggcttc
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Erwinia carotovora polygalacturonase (peh) gene.
Date: 20 Aug 92 10:01:34 GMT
Distribution: bionet
Checksum: 19732     3

LOCUS       ERWPEHB      1431 bp ds-DNA             BCT       20-AUG-1992
DEFINITION  Erwinia carotovora polygalacturonase (peh) gene.
ACCESSION   M87433
KEYWORDS    peh gene; polygalacturonase.
SOURCE      Erwinia carotovora DNA.
  ORGANISM  Erwinia carotovora
            Prokaryotae; Gram-negative facultatively anaerobic rods;
            Enterobacteriaceae.
REFERENCE   1  (bases 1 to 1431)
  AUTHORS   Lei,S.-P., Lin,H.-C., Wang,S.-S., Higaki,P. and Wilcox,G.
  TITLE     Characterization of the Erwinia carotovora peh gene and its product
            polygalacturonase
  JOURNAL   Gene 117, 119-124 (1992)
  STANDARD  full automatic
BASE COUNT      428 a    283 c    371 g    349 t
ORIGIN
        1 gagctcgccc tatgtatgtg cgatagtttt gagtttctat aaaagcgttt tagttttctt
       61 attaagttaa agaaagcgta accttttagt aaccgaaaaa ttacggacat ttataagatg
      121 aggtttgaca tggaatatca atcaggcaag cgcgttttat cattatcact gggtcttatc
      181 ggcttgttta gcgcatcggc atgggcttct gactcacgaa cggtgagtga accgaaaaca
      241 ccatcctctt gtacgacgct taaagcggac agcagcacgg caaccagtac gattcaaaaa
      301 gcgctgaata attgcgatca aggaaaagcc gtaaggttga gcgcagggag tacctctgtc
      361 tttctgagcg gtccactctc tctgccttca ggtgttagcc tattaatcga caaaggggtg
      421 accctacgcg ctgtgaataa cgccaagtct tttgagaatg cgccttcatc ctgtggtgtg
      481 gtcgataaaa atggtaaagg ctgtgatgcg tttattactg ccgtgagtac gacaaatagc
      541 ggaatctatg ggccgggcac gattgatggg cagggcggcg taaagcttca ggataaaaag
      601 gtgagctggt gggaactggc tgccgatgcc aaagtgaaaa agttaaaaca gaatactcct
      661 cggctgattc agattaataa gagtaagaat tttacgctgt ataacgtttc cttaatcaat
      721 tccccgaact tccacgtcgt ctttagcgac ggtgatgggt ttaccgcgtg gaaaaccacg
      781 attaaaacgc cgtccaccgc cagaaacacg gacggtatcg atccgatgtc gtcaaaaaat
      841 atcactattg cctacagcaa tattgcaacg ggcgacgata acgtcgcgat caaagcgtat
      901 aaaggtcggg cggaaacgcg caatatctcg atcctccata acgatttcgg tactggacat
      961 ggtatgtcga tcggcagtga aaccatgggc gtttataatg tcacggtcga tgatttgaag
     1021 atgaacggca cgaccaacgg tttgcgcatc aaaagcgata aatcggctgc cggggtggtc
     1081 aatggtgttc gatatagtaa tgtcgtgatg aagaatgtcg cgaaaccgat cgtgattgat
     1141 acggtatatg agaaaaaaga agggagtaat gtcccagact ggagtgatat cacgtttaag
     1201 gacgtgacgt ctgaaaccaa gggagtcgtg gtactgaacg gtgagaatgc taaaaaaccg
     1261 atagaggtga cgatgaagaa tgtcaaactg accagcgaca gcacatggca aatcaagaac
     1321 gtcaacgtca agaagtagtt tgattactac gttgttttgt catgacaacg ttcagagcgt
     1381 tgacaaatct acttacagaa cgagaacggt ggtaatggga tagaagagta a
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Rhesus macaque polyoma virus agnoprotein gene, complete cds.
Date: 22 Aug 92 10:01:32 GMT
Distribution: bionet
Checksum: 06312     3

LOCUS       SV4AGP2       456 bp ds-DNA             VRL       22-AUG-1992
DEFINITION  Rhesus macaque polyoma virus agnoprotein gene, complete cds.
ACCESSION   M99361
KEYWORDS    agnoprotein; enhancer.
SOURCE      Rhesus macaque polyomavirus (strain Pa-57) (library: ATCC VR-239)
            DNA.
  ORGANISM  Rhesus macaque polyomavirus
            Viridae; ds-DNA nonenveloped viruses; Papovaviridae;
            Polyomaviruses.
REFERENCE   1  (sites)
  AUTHORS   van Heuverswyn,H. and Fiers,W.
  TITLE     Nucleotide sequence of the Hind-C fragment of Simian Virus 40 DNA:
            comparison of the 5'-untranslated region of wild-type virus and of
            some deletion mutants
  JOURNAL   Eur. J. Biochem. 100, 51-60 (1979)
  STANDARD  full staff_review
REFERENCE   2  (bases 1 to 456)
  AUTHORS   Ilyinskii,P.O., Daniel,M.D., Horvath,C. and Desrosiers,R.C.
  TITLE     Genetic analysis of SV40 from brain and kidney of macaque monkeys
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     enhancer        complement(104..175)
                     /note="72-bp repeat"
     variation       replace(complement(175^176),
                     "ggtgtggaaagtccccaggctccccagcaggcagaagtatgcaaagcatgcatctca
                     attagtcagcaacca")
                     /note="2nd 72bp repeat (exists in SV40 strain 776,
                     nucleotides 179-250)"
                     /citation=[1]
     CDS             260..448
                     /product="agnoprotein"
                     /codon_start=1
                     /translation="MVLRRLSRQASVKVRRSWTESKKTAQRLFVFVLELLLQFCEGED
                     TVDGKRKKPERLTEKPES"
BASE COUNT      119 a     95 c    129 g    113 t
ORIGIN
        1 tcggcctctg cataaataaa aaaaattagt cagccatggg gcggagaatg ggcggaactg
       61 ggcggagtta ggggcgggat gggcggagtt aggggcggga ctatggttgc tgactaattg
      121 agatgcatgc tttgcatact tctgcctgct ggggagcctg gggactttcc acaccctaac
      181 tgacacacat tccacagctg gttctttccg cctcagaagg tacctaacca agttcctctt
      241 tcagaggtta tttcaggcca tggtgctgcg ccggctgtca cgccaggcct ccgttaaggt
      301 tcgtaggtca tggactgaaa gtaaaaaaac agctcaacgc ctttttgtgt ttgttttaga
      361 gcttttgctg caattttgtg aaggggaaga tactgttgac gggaaacgca aaaaaccaga
      421 aaggttaact gaaaaaccag aaagttaact ggtaag
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human chromosome 4 sequence-tagged site STS4-31
Date: 21 Aug 92 10:08:49 GMT
Distribution: bionet
Checksum: 48066     3

LOCUS       HUM4STS31     195 bp ds-DNA             PRI       21-AUG-1992
DEFINITION  Human chromosome 4 sequence-tagged site STS4-31
ACCESSION   M95322
KEYWORDS    STS; human chromosome 4; sequence tagged site.
SOURCE      Homo sapiens, clone L4-19 from the C25 lambda library (Pritchard,
            Genomics 4, 408, 1989); vector - Lambda Dash II (Stratagene)
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 195)
  AUTHORS   Goold,R.D., diSibio,G., Dugaiczyk,A., Smith,K.A., Xu,H.,
            Magrane,G., Lang,D.B., Cox,D.R., Masters,S.B. and Myers,R.M.
  TITLE     Sequence-Tagged Sites for Human Chromosome 4
  JOURNAL   Unpublished (1992) see COMMENT for author address
  STANDARD  full automatic
COMMENT     PCR components: 25 ng of human genomic DNA, 40 pmol of each
            oligonucleotide, 200 micro-M dNTPs, 0.25 U Taq  polymerase (Cetus)
            in 10 micro-l of 50 mM KCl-20 mM  Tris-HCl, pH 8.3 (at room temp),
            2.5 mM MgCl-2.  Initial denaturation at 94degC for 1.5 min, then 30
            cycles of  94degC for 15 sec, 62degC for 23 sec, and 72degC for 30
            sec, followed by a final extension at 72degC for 3.5 min, using a
            Perkin-Elmer 9600 thermocycler. PCR-amplified product size 195 bp.
            Sequence submitted by:
            Human Genome Mapping Center
            Box 0925
            University of California San Francisco
            San Francisco, CA 94143-0925  USA
            Phone: (415) 502-1612   Fax: (415) 476-8391
            e-mail: hgmcprobes@cgl.ucsf.edu
FEATURES             Location/Qualifiers
     misc_feature    1..25
                     /label=Primer1
     misc_feature    complement(172..195)
                     /label=Primer2
BASE COUNT       44 a     28 c     31 g     92 t
ORIGIN
        1 gattacgatt gaatctcctt gttaaatctg tccattttat tgtggttacc cagttgttgg
       61 catagaattg gtcatagtat tctgttataa cattttttta tttctgtgaa attgggtata
      121 atgttcctcc ttcattttta tcttagttat ttgagtcttg cttttttttt agtcactcta
      181 gctaaaggtt tacaa
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human alpha 1 collagen type II gene, exon 2.
Date: 13 Aug 92 10:00:33 GMT
Distribution: bionet
Checksum: 62400     2

LOCUS       HUMCOLAA02     30 bp ds-DNA             PRI       13-AUG-1992
DEFINITION  Human alpha 1 collagen type II gene, exon 2.
ACCESSION   M25655 J03065
KEYWORDS    collagen.
SEGMENT     2 of 5
SOURCE      Human DNA.
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 3 to 19)
  AUTHORS   Sangiorgi,F.O., Benson-Chanda,V., de Wet,W.J., Sobel,M.E.,
            Tsipouras,P. and Ramirez,F.
  TITLE     Isolation and partial characterization of the entire human pro
            alpha 1(II) collagen gene
  JOURNAL   Nucleic Acids Res. 13, 2207-2225 (1985)
  STANDARD  simple staff_entry
REFERENCE   2  (bases 1 to 2; 20 to 30)
  AUTHORS   Su,M., Bensen-Chanda,V., Vissing,H. and Ramirez,F.
  TITLE     Organization of the exons coding for pro-alpha-1(II) collagen
            N-propeptide confirms a distinct evolutionary history of this
            domain of the fibrillar collagen genes
  JOURNAL   Genomics 4, 438-441 (1989)
  STANDARD  full staff_entry
COMMENT     Draft entry and computer-readable sequence for [1] kindly provided
            by M.-W.Su, 21-NOV-1988).
FEATURES             Location/Qualifiers
     intron          <1..2
                     /note="COL2A1 intron A"
     exon            3..19
                     /number=2
                     /note="alpha-1 collagen type II precursor,
                     /hgml_locus_uid='LX0121B'"
                     /gene="COL2A1"
     intron          20..>30
                     /note="COL2A1 intron B"
BASE COUNT       10 a      6 c     10 g      4 t
ORIGIN      About 5 kb after segment 1; chromosome 12q14.3.
        1 agggcaacca ggaccaaagg taagggcttt
//