From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: divIVB region: mreB=cell shape determinant...minD=septum placement
Date: 27 Jan 93 05:52:55 GMT
Distribution: bionet
Checksum: 26237 12

LOCUS       S116569      4389 bp    DNA             BCT       12-JAN-1993
DEFINITION  divIVB region: mreB=cell shape determinant...minD=septum placement
            determinant [Bacillus subtilis, Genomic, 6 genes, 4389 nt]
ACCESSION   S47655 M95582
KEYWORDS    .
SOURCE      Bacillus subtilis
  ORGANISM  Bacillus subtilis
            Unclassified.
REFERENCE   1  (bases 1 to 4389)
  AUTHORS   Varley,A.W. and Stewart,G.C.
  TITLE     The divIVB region of the Bacillus subtilis chromosome encodes
            homologs of Escherichia coli septum placement (minCD) and cell
            shape (mreBCD) determinants.
  JOURNAL   J. Bacteriol. 174, 6729-6742 (1992)
  STANDARD  full automatic
COMMENT     This entry [NCBI gibbsq 116569] was created by the journal scanning
            component of NCBI/GenBank at the National Library of Medicine.
            This sequence comes from Fig. 2.
            Region: divIVB region.  
FEATURES             Location/Qualifiers
     CDS             326..1339
                     /note="Description: cell shape determinant;  For the
                     protein sequence (NCBI gibbsq 116570):  Method: conceptual
                     translation supplied by author.  This sequence comes from
                     Fig. 2."
                     /gene="mreB"
                     /codon_start=1
                     /translation="MFGIGARDLGIDLGTANTLVFVKGKGIVVREPSVVALQTDTKSI
                     VAVGNDAKNMIGRTPGNVVALRPMKDGVIADYETTATMMKYYINQAIKNKGMFARKPY
                     VMVCVPSGITAVEERAVIDATRQAGARDAYPIEEPFAAAIGANLPVWEPTGSMVVDIG
                     GGTTEVAIISLGGIVTSQSIRVAGDEMDDAIINYIRKTYNLMIGDRTAEAIKMEIGSA
                     EAPEESDNMEIRGRDLLTGLPKTIEITGKEISNALRDTVSTIVEAVKSTLEKTPPELA
                     ADIMDRGIVLTGGGALLRNLDKVISEETKMPVLIAEDPLDCVAIGTGKALEHIHLFKG
                     KTR"
     CDS             2..235
                     /partial
                     /note="Description: orf 5'of mreB;  For the protein
                     sequence (NCBI gibbsq 116580):  Method: conceptual
                     translation supplied by author.  This sequence comes from
                     Fig. 2."
                     /codon_start=1
                     /translation="NSSIVHPREVFKEAFKRSAASFICVHNHPSGDPTPSREDIEVTR
                     RLFECGNLIGIELLDHLVIGDKKFVSLKEKGYL"
     CDS             1370..2242
                     /note="Description: cell shape determinant;  For the
                     protein sequence (NCBI gibbsq 116571):  Method: conceptual
                     translation supplied by author.  This sequence comes from
                     Fig. 2."
                     /gene="mreC"
                     /codon_start=1
                     /translation="MPNKRLMLLLLCIIILVAMIGFSLKGGRNTTWPEKVIGDTTGVF
                     QNIFHTPAEFFAGIFENINDLKNTYKENERLREKLDGQTQYEAKLQELEEENKSLRDE
                     LGHVKSIKDYKPILATVIARSPDNWAKQVTINKGTQQNVAKDMAVTNEKGALIGKIKS
                     SGLNNFTSAVQLLSDTDRNNRVATKISGKKGSKGYGLIEGYDKEKKRLKMTIIERKDK
                     QDVKKGDLIETSGTGGVFPEGLTIGEVTDIESDSYGLTKVAYVKPAADLTDLNNVIVV
                     NRDVPTVDTEEEGS"
     CDS             2239..2757
                     /note="alternative start codon GTG.  Description: cell
                     shape determinant;  For the protein sequence (NCBI gibbsq
                     116572):  Method: conceptual translation supplied by
                     author.  This sequence comes from Fig. 2.
                     Author-given protein sequence differs from the conceptual
                     translation for reasons explained in citation"
                     /gene="mreD"
                     /codon_start=1
                     /translation="MKRFLLPFVMMLVFSAESIFTDLVHFPFVTDDQVLAPRFLMLVL
                     IFMSAFINQKHAMIYGFIFGFLYDMNYTSLLGVYMFGFAGLCYLASKAFKVLHTNAFV
                     VILIAVLAVCLLEFYVFGIQSLIHKDIMTFNGFVLDRFIPTILLNIAAALILVLPFRL
                     FFMSLKKELRDE"
     CDS             2810..3490
                     /note="alternative start codon GTG.  Description: septum
                     placement determinant;  For the protein sequence (NCBI
                     gibbsq 116573):  Method: conceptual translation supplied
                     by author.  This sequence comes from Fig. 2.
                     Author-given protein sequence differs from the conceptual
                     translation for reasons explained in citation"
                     /gene="minC"
                     /codon_start=1
                     /translation="MKTKKQQYVTIKGTKNGLTLHLDDACSFDELLDGLQNMLSIEQY
                     TDGKGQKISVHVKLGNRFLYKEQEEQLTELIASKKDLFVHSIDSEVITKKEAQQIREE
                     AEIISVSKIVRSGQVLQVKGDLLLIGDVNPGGTVRAGGNIFVLGSLKGIAHAGFNGNN
                     QAVIAASEMLPTQLRINHVLNRSPDHIQKGNEMECAYLDTDGNMVIERLQHLAHLRPD
                     LTRLEGGM"
     CDS             3492..4298
                     /note="alternative start codon TTG.  Description: septum
                     placement determinant;  For the protein sequence (NCBI
                     gibbsq 116574):  Method: conceptual translation supplied
                     by author.  This sequence comes from Fig. 2.
                     Author-given protein sequence differs from the conceptual
                     translation for reasons explained in citation"
                     /gene="minD"
                     /codon_start=1
                     /translation="MGEAIVITSGKGGVGKTTTSANLGTALAILGKRVCLVDTDIGLR
                     NLDVVMGLENRIIYDLVDVVEGRCKMHQALVKDKRFDDLLYLMPAAQTSDKTAVAPEQ
                     IKNMVQELKQEFDYVIIDCPAGIEQGYKNAVSGADKAIVVTTPEISAVRDADRIIGLL
                     EQEENIEPPRLVVNRIRNHLMKNGDTMDIDEIVQHLSIDLLGIVADDDEVIKASNHGE
                     PIAMDPKNRASIAYRNIARRILGESVPLQVLEEQNKGMMAKIKSFFGVRS"
BASE COUNT     1343 a    812 c   1056 g   1178 t
ORIGIN      
        1 gaattcatct attgtccacc cgcgagaggt gtttaaagaa gcgtttaaac gatctgccgc
       61 ttcctttatc tgtgttcata atcatccttc tggagatccg acgccgagca gggaagatat
      121 tgaagtgaca agacggctgt ttgaatgcgg aaacctgatt ggcatcgagc tgcttgacca
      181 tttggtgatc ggggataaaa aatttgtgag tttaaaggaa aaaggatatt tgtaacactt
      241 ttttttcgtc gaattaagct ataatagagt ttatgagttt ttccctttag ggtatttttg
      301 ctttaagaaa ggaagataca tacatatgtt tggaattggt gctagagacc ttggtataga
      361 tcttggaact gcgaatacgc ttgtttttgt aaaaggaaaa ggaattgttg tgagagagcc
      421 gtcagttgtc gctttgcaga cggatacgaa atcgattgtc gctgtcggaa atgatgcgaa
      481 aaatatgatt ggacggacac cgggcaacgt ggtggctctt cgcccgatga aagacggcgt
      541 tatcgctgat tatgaaacaa cggcgacgat gatgaaatat tacatcaatc aggccataaa
      601 aaataaaggc atgtttgcca gaaaaccata tgtaatggta tgtgtcccat caggcattac
      661 agctgttgaa gaacgcgctg ttatcgatgc gacaagacag gcgggagcgc gtgacgcgta
      721 tccgattgaa gagccttttg ccgcagcaat cggagccaat ctgccagttt gggaaccgac
      781 tggaagcatg gttgttgata tcgggggcgg tacgacagaa gttgcgatta tttccctcgg
      841 aggcatcgta acgtctcagt caatccgtgt agccggtgat gagatggatg acgcgattat
      901 caactacatc agaaaaacgt acaatctgat gatcggtgac cgtacggctg aagcgattaa
      961 aatggaaatc ggatctgcag aagctcctga agaatccgac aacatggaaa tccgcggccg
     1021 cgatttgctc acaggtttgc cgaaaacaat tgaaattaca ggaaaagaga tttctaacgc
     1081 tctacgcgac actgtatcta caattgtcga agcagtgaag agcacactcg aaaaaacacc
     1141 gcctgagctt gcagcagata tcatggacag aggtatagtg ttaaccggcg gcggagcgct
     1201 tttgcgcaat ttggacaaag tcatcagcga agaaacaaaa atgccggtcc ttatcgccga
     1261 agatccgctt gattgtgtag cgatcggaac agggaaagca ctggagcaca tccatctttt
     1321 caaagggaaa actagataat cgggagttca atagaagagg tgtaacacga tgccgaataa
     1381 gcggttaatg ctattacttc tgtgtattat catattggtg gctatgattg gattttcgct
     1441 gaagggcggc cgcaatacca cctggcctga gaaagtgatc ggcgatacga cgggagtatt
     1501 tcaaaatatt tttcatacgc ctgccgaatt ttttgcagga atatttgaga acatcaatga
     1561 tttaaaaaac acatacaaag aaaacgagcg tctaagagaa aaacttgacg gacagacaca
     1621 atatgaagcc aagcttcaag agcttgaaga agaaaacaaa tccttgcgtg acgagcttgg
     1681 ccatgtcaaa tcgattaaag attacaagcc gattttagca acggtcatcg ccagaagccc
     1741 tgataattgg gcgaaacagg tcaccattaa caaggggact cagcaaaacg tagcgaaaga
     1801 tatggccgtt acaaacgaaa aaggcgcatt aatcggcaag atcaaaagct ccggacttaa
     1861 caattttacg tctgctgttc agcttttaag cgatactgac cgcaataaca gagtcgcgac
     1921 aaaaatttcc ggaaaaaaag gcagcaaagg ctacggcttg atcgaaggat atgacaaaga
     1981 gaaaaaacgt cttaagatga caattattga gcgtaaggat aaacaagacg tgaaaaaagg
     2041 cgatcttatt gaaacatcag ggacaggcgg tgttttccca gaagggctga caatcggtga
     2101 agtgactgat atcgagtcag attcctatgg attaacgaag gttgcttatg taaaacctgc
     2161 ggctgacctt acagatttaa ataatgtgat cgttgttaac cgtgacgtgc cgactgtcga
     2221 tacagaggag gaaggatcgt gaaacgtttc cttctccctt tcgttatgat gcttgttttt
     2281 tctgcggaaa gcatttttac agatttggtg cattttcctt tcgttacaga tgaccaagtg
     2341 ctcgccccgc gttttttgat gcttgtattg atattcatgt cggcttttat caaccaaaaa
     2401 cacgcgatga tttacggatt tatttttggc tttctatatg acatgaacta tacaagtcta
     2461 ttaggcgttt acatgtttgg ttttgcaggg ctatgctatt tggcttcaaa agcgtttaaa
     2521 gtgttgcata caaacgcatt tgtagtgata ttgatagcag ttctggctgt ctgtctgctc
     2581 gaattttacg tattcggcat tcagtctttg attcataaag acattatgac gtttaacgga
     2641 tttgtgcttg accggtttat accgacaatt ttattaaata ttgcagcagc tcttattctt
     2701 gttctgccat ttagattgtt ttttatgagt ctaaagaaag aattgagaga tgagtaaaaa
     2761 ggattttatc tttttttgac gaaatgagta tgttgttgag gtgaatattg tgaagaccaa
     2821 aaagcagcaa tatgtaacaa taaaaggaac aaagaatgga ctaacattgc atctggatga
     2881 tgcgtgttct tttgatgagc ttctcgatgg tcttcagaat atgctgtcaa ttgaacaata
     2941 taccgatgga aaaggccaga aaatcagcgt tcatgttaag ctgggaaatc gctttttata
     3001 taaggagcaa gaggaacagc taaccgaatt gattgcgtca aagaaagatt tgtttgttca
     3061 ttctattgac agtgaagtca ttactaaaaa agaagcacag cagataagag aggaagccga
     3121 aattatttct gtttcaaaaa ttgtccgttc aggccaagtg ctgcaggtaa aaggcgactt
     3181 gctcctgatc ggtgacgtga atcccggcgg aacagtcagg gccggaggga acatttttgt
     3241 tctgggctca ctgaaaggaa ttgcgcatgc tggattcaat ggaaataatc aagcggtcat
     3301 cgccgcctct gaaatgcttc cgacacaatt aagaatcaat catgtgttaa atcgctcccc
     3361 agaccacatt caaaaaggga acgaaatgga atgtgcttat ttagatacag acggaaatat
     3421 ggtcattgaa cgccttcaac atttggctca tttaagacct gatctaacaa ggcttgaggg
     3481 aggaatgtga attgggtgag gctatcgtaa taacttcggg aaaaggcgga gtaggtaaga
     3541 caacaacatc tgcgaacctc ggtaccgcct tagccatttt agggaagcgc gtatgcttag
     3601 tagatactga tataggactg cgcaaccttg atgttgtaat gggtcttgaa aatagaatta
     3661 tttacgatct ggtagacgtt gtagagggca gatgcaaaat gcatcaggcg ctcgtaaaag
     3721 acaaacgttt cgatgatctg ctctatttaa tgcccgctgc tcaaacgagc gataagacag
     3781 ctgttgctcc tgaacaaatt aaaaacatgg tccaagagct caaacaggaa tttgactatg
     3841 tcatcataga ctgtcctgcc ggaatcgagc aagggtacaa aaatgccgtt tccggagctg
     3901 ataaagcgat tgtggtcact acgcctgaaa tctcagctgt tcgtgatgct gaccgtatta
     3961 taggactgct ggagcaagag gaaaatattg aaccgccgcg gctcgttgtt aacagaatca
     4021 gaaatcacct gatgaaaaac ggtgacacga tggatatcga cgaaatcgta cagcatctgt
     4081 cgatcgattt gctcggaatc gtggctgatg atgatgaagt cattaaagct tccaatcatg
     4141 gcgaaccgat tgcgatggac cctaaaaacc gcgcttccat tgcatatcgc aatattgccc
     4201 gccgcatctt aggtgaatct gttcctttac aggtgcttga agagcaaaac aaaggaatga
     4261 tggctaagat taagtcattt ttcggagtaa gatcttaatg tgatagaatc aaagagaaga
     4321 atctgacaaa gcatatgctg tgtcaggttt tttttgtttt tgcctgcttt gttcttgact
     4381 aaaccgaat
//