From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human rearranged immunoglobulin lambda light chain mRNA
Date: 15 Aug 92 10:02:14 GMT
Distribution: bionet
Checksum: 02575     4

LOCUS       HUMIGVL032    802 bp ss-RNA             PRI       15-AUG-1992
DEFINITION  Human rearranged immunoglobulin lambda light chain mRNA
ACCESSION   X57822
KEYWORDS    Ig lambda light chain; immunoglobulin.
SOURCE      Homo sapiens RNA.
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 802)
  AUTHORS   Klobeck,H.G.
  JOURNAL   Unpublished (1991)
  STANDARD  full automatic
REFERENCE   2  (bases 342 to 388)
  AUTHORS   Combriato,G. and Klobeck,H.G.
  TITLE     V(lambda) and J(lambda)-C(lambda) gene segments of the human
            immunoglobulin lambda light chain locus are separated by 14kb and
            rearrange by a deletion mechanism
  JOURNAL   Eur. J. Immunol. 21, 1513-1522 (1991)
  STANDARD  full automatic
COMMENT     *source: tissue=spleen; *source: cell_type=B-cell; *source:
            clone_library=phage library cML; *source: clone=cML93; *source:
            isolate=individual ML; *source: chromosome=22; *source:
            is_germline=N;
            for overlapping sequences see: X51754-55; J00252-54; M15641-42
            From EMBL    entry HSIGVL032;  dated 15-JUL-1991.
FEATURES             Location/Qualifiers
     CDS             <1..706
                     /gene="immunoglobulin lambda light chain"
                     /codon_start=2
                     /translation="ASFPLLLTLLTHCAGSWAQSVLTQPPSASGTPGQRVTISCSGST
                     SNIGSNTVNWYQQLPGTAPKVLIYNNNERPSGVPDRFSGSKSGTSASLAISGLQSDDE
                     ADYYCAAWDDSLNGRLLGGGTKLTVLGQPKAAPSVTLFPPSSEELQANKATLVCLISD
                     FYPGAVTVAWKADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHKSYSCQVT
                     HEGSTVEKTVAPTECS"
     sig_peptide     <1..55
                     /gene="immunoglobulin lambda light chain"
                     /codon_start=2
     mRNA            1..802
                     /note="cDNA"
                     /gene="immunoglobulin lambda light chain"
                     /evidence=EXPERIMENTAL
     misc_feature    56..386
                     /note="V(lambda)I"
                     /gene="immunoglobulin lambda light chain"
     misc_feature    356..386
                     /note="J-segment"
                     /gene="immunoglobulin lambda light chain"
     misc_feature    387..706
                     /note="constant region"
                     /gene="immunoglobulin lambda light chain"
BASE COUNT      177 a    269 c    206 g    150 t
ORIGIN
        1 ggccagcttc cctctcctcc tcaccctcct cactcactgt gcagggtcct gggcccagtc
       61 tgtgctgact cagccaccct cagcgtctgg gacccccggg cagagggtca ccatctcttg
      121 ttctggaagc acctccaaca tcggaagtaa tactgtgaac tggtaccagc aactcccagg
      181 aacggccccc aaagtcctca tctataataa taatgagcgg ccctcagggg tccctgaccg
      241 tttctctggc tccaagtctg gcacctcagc ctccctggcc atcagtgggc tccagtctga
      301 cgatgaggct gattattact gtgcagcatg ggatgacagt ctgaatggcc ggcttttggg
      361 cggagggacc aagttgaccg tcctaggtca gcccaaggct gccccctcgg tcactctgtt
      421 cccaccctcc tctgaggagc ttcaagccaa caaggccaca ctggtgtgtc tcataagtga
      481 cttctacccg ggagccgtga cagtggcctg gaaggcagat agcagccccg tcaaggcggg
      541 agtggagacc accacaccct ccaaacaaag caacaacaag tacgcggcca gcagctacct
      601 gagcctgacg cctgagcagt ggaagtccca caaaagctac agctgccagg tcacgcatga
      661 agggagcacc gtggagaaga cagtggcccc tacagaatgt tcataggttc tcatccctca
      721 ccccccacca cgggagacta gagctgcagg atcccagggg aggggtctct cctcccaccc
      781 caaggcatca agcccttctc cc
//