From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Lactococcus lactis lactate dehydrogenase (ldh) gene, complete cds.
Date: 29 Dec 92 21:38:21 GMT
Distribution: bionet
Checksum: 47107 4

LOCUS       LACLACD      1368 bp ds-DNA             BCT       15-DEC-1992
DEFINITION  Lactococcus lactis lactate dehydrogenase (ldh) gene, complete cds.
ACCESSION   M95919
KEYWORDS    lactate dehydrogenase.
SOURCE      Lactococcus lactis DNA.
  ORGANISM  Lactococcus lactis
            Prokaryotae; Firmicutes; Firmibacteria; Gram-positive cocci;
            Streptococcaceae.
REFERENCE   1  (bases 1 to 1368)
  AUTHORS   Llanos,R.M., Hillier,A.J. and Davidson,B.E.
  TITLE     Cloning, nucleotide sequence, expression, and chromosomal location
            of ldh, the gene encoding L-(+)-lactate dehydrogenase from
            Lactococcus lactis
  JOURNAL   J. Bacteriol. 174, 6956-6964 (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     RBS             98..104
                     /gene="ldh"
     stem_loop       1097..1140
                     /function="putative transcription terminator"
                     /gene="ldh"
                     /note="putative"
     stem_loop       1167..1202
                     /function="putative transcription terminator"
                     /gene="ldh"
                     /note="putative"
     CDS             118..1095
                     /EC_number="1.1.1.27"
                     /gene="ldh"
                     /evidence="EXPERIMENTAL"
                     /citation=[1]
                     /product="lactate dehydrogenase"
                     /codon_start=1
                     /translation="MADKQRKKVILVGDGAVGSSYAFALVNQGIAQELGIVDLFKEKT
                     QGDAEDLSHALAFTSPKKIYSADYSDASDADLVVLTSGAPQKPGETRLDLVEKNLRIT
                     KDVVTKIVASGFKGIFLVAANPVDILTYATWKFSGFPKNRVVGSGTSLDTARFRQALA
                     EKVDVDARSIHAYIMGEHGDSEFAVWSHANVAGVKLEQWFQENDYLNEAEIVELFESV
                     RDAAYSIIAKKGATFYGVAVALARITKAILDDEHAVLPVSVFQDGQYGVSDFYLGQPA
                     VVGAEGVVNPIHIPLNDAEMQKMEASGAQLKAIIDEAFAKEEFASAVKN"
BASE COUNT      420 a    252 c    270 g    426 t
ORIGIN      
        1 tttgtcaatt aatgatctta aaacaataca gcctgccgct atcgacaggc cctattgttg
       61 aaaagattat attttataaa atactcgttt ataatttgaa aggaattaaa gaaaatcatg
      121 gctgataaac aacgtaagaa agttatcctt gttggtgacg gtgctgtagg ttcatcatac
      181 gcttttgccc ttgttaacca aggaattgca caagaattag gtattgttga cctttttaaa
      241 gaaaaaactc aaggggatgc agaagacctt tctcatgcct tggcatttac atcacctaaa
      301 aagatttact ctgcagacta ctctgatgca agcgacgctg acctcgttgt cttgacttct
      361 ggtgctccac aaaaaccagg tgaaactcgt cttgaccttg ttgaaaaaaa tcttcgtatt
      421 actaaagatg ttgtaactaa aattgttgct tcaggattca aaggaatctt cctcgttgct
      481 gctaacccag ttgacatctt gacatacgca acttggaaat tctctggttt ccctaaaaac
      541 cgtgttgtag gttcaggtac ttcacttgat actgcacgtt tccgtcaagc attggctgaa
      601 aaagttgacg ttgatgctcg ttcaatccac gcatacatca tgggtgaaca cggtgactca
      661 gaatttgctg tttggtcaca cgctaacgtt gctggtgtta aattggaaca atggttccaa
      721 gaaaatgact accttaacga agcagaaatc gttgaattgt ttgagtctgt acgtgatgca
      781 gcttactcaa tcatcgctaa aaaaggtgca acattctacg gtgtggctgt agcccttgct
      841 cgtattacta aagcaattct tgatgatgaa catgcagtac ttcctgtatc agtattccaa
      901 gatggacaat atggggtaag cgacttctac cttggtcaac cagctgtagt tggtgctgaa
      961 ggtgttgtta acccaattca cattccattg aacgatgctg aaatgcaaaa aatggaagct
     1021 tctggagctc aattgaaagc tatcatcgat gaagcttttg ctaaagaaga atttgcttct
     1081 gcagttaaaa actaaaaata aaaaagagtt agtagaaaat ttcaactaac tctttttatt
     1141 atatttaaaa gtttgcttta aactataata aaaaagtctg ttcatcgaca gactttttta
     1201 tttttagaaa tttatttgga ttttatatag tttaccccgt cagctttagg cgcaattgct
     1261 tttccaagga agagtgccaa gacgataatt gtaaagacgt aaggtgccat ttgtaggaat
     1321 gaagaaggga tttctttgat tcctgggatt tgaccaccga caacggcc
//