From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Escherichia coli cyclopropane fatty acid synthase (cfa) gene,
Date: 27 Jan 93 05:47:21 GMT
Distribution: bionet
Checksum: 20131 4

LOCUS       ECOCFAX      1575 bp ds-DNA             BCT       26-JAN-1993
DEFINITION  Escherichia coli cyclopropane fatty acid synthase (cfa) gene,
            complete cds.
ACCESSION   M98330
KEYWORDS    cyclopropane fatty acid synthetase.
SOURCE      Escherichia coli (strain K-12) HfrC DNA.
  ORGANISM  Escherichia coli
            Prokaryotae; Gram-negative facultatively anaerobic rods;
            Enterobacteriaceae.
REFERENCE   1  (bases 1 to 1575)
  AUTHORS   Wang,A.-Y., Grogan,D.W. and Cronan,J.E.Jr.
  TITLE     Cyclopropane fatty acid synthase of Escherichia coli: deduced amino
            acid sequence, purification, and studies of the enzyme active site
  JOURNAL   Biochemistry 31, 11020-11028 (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     CDS             286..1434
                     /gene="cfa"
                     /evidence="EXPERIMENTAL"
                     /product="cyclopropane fatty acid synthase"
                     /codon_start=1
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
BASE COUNT      383 a    355 c    385 g    452 t
ORIGIN      
        1 atcgatatac ttatacttag gctgctaaca aaattttgtt gtatgattga aattagcggc
       61 ctatactaat ttcgagttgt taaagctacg ataaatatta tgtttttacg gggacaggat
      121 cgttcccgac tcactatgga tagtcatttc ggcaagggtt cctcctttcc ctctgttcta
      181 cgtcggatta tagactcgcg gttttttctg cgagatttct cacaaagccc aaaaagcgtc
      241 tacgctgttt taaggttctg atcaccgacc agtgatggag aaactatgag ttcatcgtgt
      301 atagaagaag tcagtgtacc ggatgacaac tggtaccgta tcgccaacga attacttagc
      361 cgtgccggta tagccattaa cggttctgcc ccggcggata ttcgtgtgaa aaaccccgat
      421 ttttttaaac gcgttctgca agaaggctct ttggggttag gcgaaagtta tatggatggc
      481 tggtgggaat gtgaccgact ggatatgttt tttagcaaag tcttacgcgc aggtctcgag
      541 aaccaactcc cccatcattt caaagacacg ctgcgtattg ccggcgctcg tctcttcaat
      601 ctgcagagta aaaaacgtgc ctggatagtc ggcaaagagc attacgattt gggtaatgac
      661 ttgttcagcc gcatgcttga tcccttcatg caatattcct gcgcttactg gaaagatgcc
      721 gataatctgg aatctgccca gcaggcgaag ctcaaaatga tttgtgaaaa attgcagtta
      781 aaaccaggga tgcgcgtact ggatattggc tgcggctggg gcggactggc acactacatg
      841 gcatctaatt atgacgtaag cgtggtgggc gtcaccattt ctgccgaaca gcaaaaaatg
      901 gctcaggaac gctgtgaagg cctggatgtc accattttgc tgcaagatta tcgtgacctg
      961 aacgaccagt ttgatcgtat tgtttctgtg gggatgttcg agcacgtcgg accgaaaaat
     1021 tacgatacct attttgcggt ggtggatcgt aatttgaaac cggaaggcat attcctgctc
     1081 catactatcg gttcgaaaaa aaccgatctg aatgttgatc cctggattaa taaatatatt
     1141 tttccgaacg gttgcctgcc ctctgtacgc cagattgctc agtccagcga accccacttt
     1201 gtgatggaag actggcataa cttcggtgct gattacgata ctacgttgat ggcgtggtat
     1261 gaacgattcc tcgccgcatg gccagaaatt gcggataact atagtgaacg ctttaaacga
     1321 atgtttacct attatctgaa tgcctgtgca ggtgctttcc gcgcccgtga tattcagctc
     1381 tggcaggtcg tgttctcacg cggtgttgaa aacggccttc gagtggctcg ctaaaggcta
     1441 ttctatcgcc ccctctccgg gggcgatttc agatcaggct tctgtgcctg gttgattcat
     1501 ggcattttct cgtgccgcca gcacacgttc taccgtatct accactgcct gagtttgtgg
     1561 atcgatttca atgtt
//