From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Nitrosomonas europaea ammonia monooxygenase acetylene-binding
Date: 31 Dec 92 01:46:36 GMT
Distribution: bionet
Checksum: 01416 5

LOCUS       NTOAMOAB     1883 bp ds-DNA             BCT       18-DEC-1992
DEFINITION  Nitrosomonas europaea ammonia monooxygenase acetylene-binding
            protein (amoA) gene, complete cds; ammonia monooxygenase (amoB)
            gene, partial cds.
ACCESSION   L08050
KEYWORDS    acetylene-binding protein; ammonia monooxygenase; amoA gene;
            amoB gene.
SOURCE      Nitrosomonas europaea DNA.
  ORGANISM  Nitrosomonas europaea
            Prokaryotae; Gracilicutes; Proteobacteria; Aerobic chemolithotroph;
            Nitrobacteriaceae.
REFERENCE   1  (bases 1 to 1883)
  AUTHORS   McTavish,H.E., Fuchs,J.A. and Hooper,A.B.
  TITLE     Cloning and sequencing of the gene for ammonia monooxygenase of
            Nitrosomonas europaea
  JOURNAL   J. Bacteriol. (1992) In press
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     CDS             <1192..>1883
                     /gene="amoB"
                     /evidence="EXPERIMENTAL"
                     /product="ammonia monooxygenase"
                     /codon_start=1
                     /translation="HGERSQEPFLRMRTVQWYDIKWGPEVTKVNENAKITGKFHLAED
                     WPRAAAQPDFSFFNVGSPSPVFVRLSTKINGHPWFISGPLQIGRDYEFEVNLRARIPG
                     RHHMHAMLNVKDAGPIAGPGAWMNITGSWDDFTNPLKLLTGETIDSETFNLSNGIFWH
                     VVWMSIGIFWIGVFTARPMFLPRSRVLLAYGDDLLMDPMDKKITWVLAILTLALVWGG
                     YRYTENKHPYTVP"
     CDS             249..1079
                     /gene="amoA"
                     /evidence="EXPERIMENTAL"
                     /product="ammonia monooxygenase acetylene-binding protein"
                     /codon_start=1
                     /translation="MSIFRTEEILKAAKMPPEAVHMSRLIDAVYFPILIILLVGTYHM
                     HFMLLAGDWDFWMDWKDRQWWPVVTPIVGITYCSAIMYYLWVNYRQPFGATLCVVCLL
                     IGEWLTRYWGFYWWSHYPINFVTPGIMLPGALMLDFTLYLTRNWLVTALVGGGFFGLL
                     FYPGNWPIFGPTHLPIVVEGTLLSMADYMGHLYVRTGTPEYVRHIEQGSLRTFGGHTT
                     VIAAFFSAFVSMLMFTVWWYLGKVYCTAFFYVKGKRGRIVHRNDVTAFGEEGFPEGIK
                     "
BASE COUNT      460 a    393 c    502 g    528 t
ORIGIN      
        1 gatcattacc cgttattcca atctgaccga tgtggtttgg aacaaccaaa gcaaagaaat
       61 tctgaataac cggattgtag cttaatctgg tatgtactcc aggcagaagt tgcgcttggg
      121 gtacattaaa agaggaagtc tggccgggtg ctgaaagtag cacctggtca gtcagaacca
      181 gagtgcggta ccttagagga aaagaggaat cgtaatgtta cttttaaact ataagagggg
      241 agggcagggt gagtatattt agaacggaag aaatcctgaa agcggccaag atgccgccgg
      301 aagcggttca tatgtcacgt ctgattgatg cagtttattt tccaattctg attattttgc
      361 tggtgggtac ctaccacatg cactttatgc tgctggcagg tgactgggat ttctggatgg
      421 actggaaaga tcgtcaatgg tggccggttg taacgccaat cgtggggatc acctactgtt
      481 cggcaatcat gtattacttg tgggtcaact accgccaacc gtttggtgca acgttgtgtg
      541 tggtgtgtct gctgattggt gagtggctga cacgttactg gggattctac tggtggtcac
      601 actaccccat caacttcgta acaccgggca ttatgcttcc gggtgcgctg atgctggact
      661 tcacgctgta tctgacacgc aactggctgg tgacggctct ggttggaggt ggattcttcg
      721 gtctgctgtt ctatccgggt aactggccga tttttggacc aacccatttg ccaatcgttg
      781 tagaaggcac attgctgtcg atggctgatt acatgggaca tctgtatgtt cgtacaggta
      841 cacccgagta tgttcgtcat attgagcaag gttcactgcg tacctttggt ggtcatacca
      901 cagttattgc agcattcttc tctgcgttcg tatcaatgtt gatgttcacc gtatggtggt
      961 atcttggaaa agtttactgt acagcctttt tctacgttaa aggtaaaaga ggtcgtatcg
     1021 tacatcgcaa tgatgttacc gcattcggtg aagaaggctt tccagagggg atcaaataaa
     1081 atgggtatca aaaaccttta taaacgggga gtgatggggc tttacggtgt tgcttatgca
     1141 gtagccgcat tagccatgac agtaacattg gatgtctcga cggtagcagc tcatggggag
     1201 cgatcccagg agccgttcct tcggatgcgt acagttcaat ggtatgacat caaatggggt
     1261 cctgaggtaa ccaaagttaa tgagaacgca aaaatcaccg gtaagtttca cttggctgaa
     1321 gattggccac gtgcagctgc acaacctgat ttttccttct ttaacgtagg aagcccaagc
     1381 ccggtattcg tacgtttgag taccaagatc aatggccatc catggttcat ttcaggtcct
     1441 ctgcaaattg gccgtgacta tgagtttgaa gtcaatctgc gtgcacgtat tccgggacgt
     1501 catcacatgc atgccatgct gaacgtcaaa gatgcaggtc cgattgctgg gccaggtgca
     1561 tggatgaaca ttaccggaag ctgggatgat tttaccaacc cactgaaatt attgacgggt
     1621 gagacaattg attcagaaac cttcaactta tcgaacggta tattctggca cgttgtctgg
     1681 atgtccatcg gtattttctg gattggtgtt tttacagcac ggccgatgtt cctgccacgt
     1741 agtcgtgtat tgttggccta cggtgatgat ctgttgatgg atccgatgga caaaaaaatc
     1801 acctgggtac ttgccattct gacgctggcg ctggtatggg gtggataccg ttatacggaa
     1861 aataaacatc cctacacggt acc
//