From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: D.pseudoobscura Adh gene for alcohol dehydrogenase
Date: 28 Dec 92 21:26:09 GMT
Distribution: bionet
Checksum: 50362 8

LOCUS       DROADHGN01   3494 bp ds-DNA             INV       01-DEC-1992
DEFINITION  D.pseudoobscura Adh gene for alcohol dehydrogenase
ACCESSION   X62181
KEYWORDS    ADH gene; alcohol dehydrogenase.
SOURCE      Drosophila pseudoobscura DNA.
  ORGANISM  Drosophila pseudoobscura
            Eukaryota; Animalia; Metazoa; Arthropoda; Uniramia; Insecta;
            Pterygota; Neoptera; Holometabola; Diptera; Brachycera;
            Cyclorrhapha; Schizophora; Drosophiloidea; Drosophilidae.
REFERENCE   1  (bases 1 to 3494)
  AUTHORS   Schaeffer,S.W. and Miller,E.L.
  TITLE     Estimates of gene flow in Drosophila pseudoobscura determined from
            nucleotide sequence analysis of the alcohol dehydrogenase region
  JOURNAL   Genetics 132, 471-480 (1992)
  STANDARD  full automatic
COMMENT     *source: strain=BC414; *source: isolate=British Columbia, Canada;
            *source: chromosome=IV section 88;
               From EMBL    entry DPADHGN01;  dated 16-OCT-1992.
FEATURES             Location/Qualifiers
     mRNA            join(40..119,923..1028,1092..1496,1557..1972)
                     /note="adult mRNA (Adh)"
     mRNA            join(890..1028,1092..1496,1557..1972)
                     /note="larval mRNA (Adh)"
     promoter        8..14
                     /note="adult promoter (Adh)"
     prim_transcript 40..1972
                     /note="adult primary transcript (Adh)"
     exon            40..119
                     /note="adult exon 1a"
     intron          120..922
                     /note="adult intron"
     promoter        854..861
                     /note="larval promoter (Adh)"
     prim_transcript 890..1972
                     /note="larval primary transcript (Adh)"
     exon            890..1028
                     /note="larval exon 1"
     exon            923..1028
                     /note="adult exon 1b"
     intron          1029..1091
                     /number=1
     exon            1092..1496
                     /number=2
     intron          1497..1556
                     /number=2
     exon            1557..1972
                     /number=3
     polyA_signal    1949..1954
     polyA_site      1972
     CDS             join(936..1028,1092..1496,1557..1823)
                     /EC_number="1.1.1.1"
                     /gene="Adh"
                     /product="alcoholdehydrogenase"
                     /codon_start=1
                     /translation="MSLTNKNVVFVAGLGGIGLDTSRELVKRNLKNLVILDRIDNPAA
                     IAELKAINPKVTITFYPYDVTVPVAETTKLLKTIFAQVKTIDVLINGAGILDDHQIER
                     TIAVNYTGLVNTTTAILDFWDKRKGGPGGIICNIGSVTGFNAIYQVPVYSGSKAAVVN
                     FTSSLAKLAPITGVTAYTVNPGITKTTLVHKFNSWLDVEPRVAEKLLEHPTQTSQQCA
                     ENFVKAIELNKNGAIWKLDLGTLEPITWTQHWDSGI"
BASE COUNT      973 a    796 c    804 g    921 t
ORIGIN      
        1 cgtagtgtat ttaaggcact gcacatgtcg aggaccacga ttattgtcta cagtgcagtc
       61 agtagttgtc aggtgcagca ggcggaccag catttcttta aatctactca atcgatcaag
      121 taagtaactg taatccaagt acagtcggcc catcccactc ggtcactcgg ttctgccgta
      181 actcaagaaa tttcttgata gcgcagttcc agttgtagaa cgtcgtttaa aaaaacatct
      241 cgttggagct tgggttgaaa aacgttcgag ttgggcgtag aaaatttcaa atataaaata
      301 tcaaaaaaga gaaaatatct tcccaaagca gctgtccatt ttttgttaaa gtgatagatc
      361 aaaaattcgg atgacgtaca ttcctagtgc acttcataca aataatttcc tcaattctaa
      421 ttctacaaga agcacttttg gcacttgtcc agaaaaaaca atttgtttac acttttcggt
      481 tgataacaga ggcggaccga cggctaagta acataacagt cgcctgttaa gtaatgccct
      541 cactctctgt tagatggccg cgcgtgtcga ctccgataga ccagcagaca cgcagtagtt
      601 ttcctcaaga acctgaactc taaacataga cataatttac gataccttga gactcaaata
      661 aaaatacaag tatgagtggt attctattcc tgattagact cgcacacaca cacgtacgag
      721 tattaacata aatttgccgg caaataacca aaacaaaacg aacgcaaacg atccgaacac
      781 gtaatgcgag agataagaaa cagaaaagct cgacgtgaaa gaagcttctg acggaagctc
      841 ttgccgaact ccctataaat acgagacaga aaaccagaat aaatataata acaaactgtt
      901 gaattgtccc atcgattccc agatcaaaag acagaatgtc tctcaccaac aagaacgtcg
      961 ttttcgtggc cggtctgggc ggcattggcc tggacaccag ccgggagttg gtcaagcgta
     1021 atctgaaggt aagagagaac gaattccatg gaatctatgg aatcctaaat ttaaaaattt
     1081 atatttttta gaacctggtc atcctggatc gcattgacaa tccggctgcc attgccgaac
     1141 tgaaggcaat caatcccaag gtgaccatca ccttctatcc ctacgatgtg actgtgcccg
     1201 tcgctgagac caccaagctc ctgaagacca tctttgccca ggtgaagaca atcgatgtcc
     1261 tgatcaacgg tgctggcatc ctggacgatc atcagatcga gcgcaccatt gccgttaact
     1321 acacgggcct ggtcaacacc accacagcca ttctggactt ctgggacaag cgcaagggcg
     1381 gcccaggcgg catcatttgc aacattggct ccgtcaccgg tttcaatgcc atctaccagg
     1441 tgcccgttta ctccggctcc aaggcggcgg tggttaactt tacctcctcc ctggcggtaa
     1501 gtaatccaac gtttttccta ctccccaaaa ctaaatattt ctttgaaatt ccatagaaac
     1561 tggctcccat tactggtgtc actgcttaca ctgtcaatcc tggcatcacg aagaccactc
     1621 tggtccacaa attcaactcg tggctggatg tggagccccg tgtggcggag aagctgctcg
     1681 agcatcccac ccagacctct cagcagtgcg ccgagaactt tgtgaaggcc attgagctga
     1741 acaagaacgg tgccatctgg aagttggatc tgggcacctt ggagcccatc acatggaccc
     1801 agcactggga ctcgggcatc taaaccatcc cagagactct atgggacatg gcgttttagc
     1861 ttttagtttc tttttttcca ctcaattgtt acgtatatac atacacatat ggcaataagg
     1921 ctgatttgaa ccccgttttg aatatgataa tacaaaatta tatttgagaa atttcaacaa
     1981 aatcgataag agctatagct ctcttttaat taaaaaaaac tagataaaaa gagcaatggt
     2041 cagtggtggt cagtggtggt cagtggtcgt tgtggcctct atcgatttcc acacaaaaac
     2101 tttactgttt agtaatagaa aagaacttga gaaaggcagc caaaagaatg tacgatctga
     2161 cgggtaagca tgtctgctat gtggctgact gcggtggcat tgcactggag accagcaagg
     2221 ttctcatgac caagaatata gcggtgagtg gtagagtggt gtagagagtg gagagtgttc
     2281 cgaagtggtt ctggttctta aacaagtctc tagtctctag tctcttggct gctgctggta
     2341 gctgtcgaaa ctaattaagt taatgacttt ttcatgtttt gtttttgtcc gctgactttt
     2401 gctgttcaaa gctacaatta agtttatgtt ttgagtttga tctacttttg cttaagggaa
     2461 gtactttcga ttacggattg ctggcatata aaaatagata ccaaggaatg tgcatcatat
     2521 catattgatt ctatcttgat tctttgccca gaaactggcc atccttcaga gcgtggaaaa
     2581 ccccccggcc atcgcccagt tacaatcgat taagcacagc acacagatct tcttctggac
     2641 cttcgatgtg accatggccc gcgaggagat gaagaagtac ttcgatgagg tcatggtcca
     2701 gatggactac atcgatgtgc tgatcaatgg ggccaccctg tgcgatgagc ggaacattga
     2761 tgccaccatc aatacgaatc tgaccggcat gatgaacacc gtggccactg tgctgcccta
     2821 tatggaccga aagatgggcg gttcgggtgg actgattgtg aatgtcacct ccgtcatagg
     2881 actggatcca tcgccggtct tttgtgcgta cagtgcctca aagtttggtg tgattgggtt
     2941 cacccgaagt ctagcggtga gtgggcgaga tcttagcttg aaaatgctct ctaatgtctt
     3001 tcattctttc gcaggatccc ctgtatttca cccaaaatgg tgtggctgtc atggccgtct
     3061 gctgtggtcc caccaaagtg tttgtcgatc gggaactgac tgccttcctg gcatacggcc
     3121 agtcctttgc cgatcgcctg cgtcgtgccc cctgccaatc gacggccgtc tgcggccaga
     3181 atatagtaaa tgccatcgag agatcggaga acggacagat ctggattgcc gacaagggcg
     3241 gcctggagtc ggtggccctc cactggtatt ggcatatggc cgatcagttt gtgaactata
     3301 tgcagagcac tgacgatgaa gatcaggagt tttttttggg ccagcgatga ggcatgcctt
     3361 gatccacgga ataaaacatc aaatgtgtca caaaaagtag gcaacttgtt tgtgtgtttg
     3421 gaaatagatt tgaaatattt tgaggtcata tgaataatga cataaatgag ggtgcctgaa
     3481 agtatgccaa gttt
//