From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: D.pseudoobscura Adh gene for alcohol dehydrogenase
Date: 28 Dec 92 21:27:44 GMT
Distribution: bionet
Checksum: 29589 7

LOCUS       DROADHGN63   3453 bp ds-DNA             INV       01-DEC-1992
DEFINITION  D.pseudoobscura Adh gene for alcohol dehydrogenase
ACCESSION   X64472
KEYWORDS    ADH gene; alcohol dehydrogenase.
SOURCE      Drosophila pseudoobscura DNA.
  ORGANISM  Drosophila pseudoobscura
            Eukaryota; Animalia; Metazoa; Arthropoda; Uniramia; Insecta;
            Pterygota; Neoptera; Holometabola; Diptera; Brachycera;
            Cyclorrhapha; Schizophora; Drosophiloidea; Drosophilidae.
REFERENCE   1  (bases 1 to 3453)
  AUTHORS   Schaeffer,S.W. and Miller,E.L.
  TITLE     Estimates of gene flow in Drosophila pseudoobscura determined from
            nucleotide sequence analysis of the alcohol dehydrogenase region
  JOURNAL   Genetics 132, 471-480 (1992)
  STANDARD  full automatic
COMMENT     *source: strain=PS214; *source: isolate=Kiabab National Forest,
            Arizona; *source: chromosome=IV section 88;
               From EMBL    entry DPADHGN63;  dated 16-OCT-1992.
FEATURES             Location/Qualifiers
     mRNA            join(40..119,913..1018,1083..1487,1548..1963)
                     /note="adult mRNA (Adh)"
     mRNA            join(880..1018,1083..1487,1548..1963)
                     /note="larval mRNA (Adh)"
     promoter        8..14
                     /note="adult promoter (Adh)"
     prim_transcript 40..1963
                     /note="adult primary transcript (Adh)"
     exon            40..119
                     /note="adult exon 1a"
     intron          120..912
                     /note="adult intron"
     prim_transcript 880..1963
                     /note="larval primary transcript (Adh)"
     exon            880..1018
                     /note="larval exon 1"
     exon            913..1018
                     /note="adult exon 1b"
     intron          1019..1082
                     /number=1
     exon            1083..1487
                     /number=2
     intron          1488..1547
                     /number=2
     exon            1548..1963
                     /number=3
     polyA_signal    1940..1945
     polyA_site      1963
     CDS             join(926..1018,1083..1487,1548..1814)
                     /EC_number="1.1.1.1"
                     /gene="Adh"
                     /product="alcoholdehydrogenase"
                     /codon_start=1
                     /translation="MSLTNKNVVFVAGLGGIGLDTSRELVKRNLKNLVILDRIDNPAA
                     IAELKAINPKVTITFYPYDVTVPVAETTKLLKTIFAQVKTIDVLINGAGILDDHQIER
                     TIAVNYTGLVNTTTAILDFWDKRKGGPGGIICNIGSVTGFNAIYQVPVYSGSKAAVVN
                     FTSSLAKLAPITGVTAYTVNPGITKTTLVHKFNSWLDVEPRVAEKLLEHPTQTSQQCA
                     ENFVKAIELNKNGAIWKLDLGTLEPITWTQHWDSGI"
BASE COUNT      971 a    786 c    790 g    906 t
ORIGIN      
        1 cgtagtgtat ttaaggcact gcacatgtcg aggaccacga ttattgtcta cagtgcagtc
       61 agtagttatc aggtgcagca ggcggaccag catttcttta aatctactca atcgatcaag
      121 taagtaacta atccaagtac agtcggccca tcccactcgg ttctgccgta gctcaagaaa
      181 tttcttgata gcgcagttcc agttgtagaa cgtcgcttaa aaaaacatct cattggagct
      241 tgggttgaaa aacgttcgag ttgggcgtag aaaagttcaa atataaaata tcaaaaaaga
      301 gaaaatatct tcccaaagca gctgtccatt ttttgtttaa gtgatagatc aaaaattcgg
      361 atgacgtaca ttcctagtgc acttcataca aataatttcc tcaattctaa ttctacaaga
      421 agcacttttg gctcttgtcc agaaaaaaca atttgtttac acttttcggt tgataacaga
      481 ggcggaccga cggctaagta acataacagt cgcctgttaa gtaatgccct cactctctgt
      541 tagatggccg cgcgtgtcga ctccgataga ccagcagaca cgcagtagtt ttcctcaaga
      601 acctgaactc taaacataga cataatttac gataccttga gactcaaata aaaatacaag
      661 tatgagtggt attctattcc tgattagact cgcacacaca cacgtacgag tattaacata
      721 aatttgccgg caaataacca aaacaaaacg aacgcaaacg atccgaacac gtaatgcgag
      781 agataagaaa cagaaaagct cgacgtgaaa gaagcttctg gcggaagctc ttgccgaact
      841 ccctataaat acgagacaga aaaccagaat aaatataata acaaactgtt gaattgtccc
      901 atcgattccc agatcaaaag acagaatgtc tctcaccaac aagaacgtcg ttttcgtggc
      961 cggtctgggc ggcattggcc tggacaccag ccgggagttg gtcaagcgta atctgaaggt
     1021 aagagagaac aaattccatg gaatctatgg aaatcctaaa tttaaaaatt tatttttttt
     1081 agaacctggt catcctggat cgcattgaca atccggctgc cattgccgaa ctgaaggcaa
     1141 tcaatcccaa ggtgaccatc accttctatc cctacgatgt gactgtgccc gtcgctgaga
     1201 ccaccaagct cctgaagacc atctttgccc aggtgaagac aatcgatgtc ctgatcaacg
     1261 gtgctggcat cctggacgat catcagatcg agcgcaccat tgccgttaac tacacgggcc
     1321 tggtcaacac caccacagcc attctggact tctgggacaa gcgcaagggc ggcccaggcg
     1381 gcatcatttg caacattggc tctgtcaccg gtttcaatgc catctaccag gtgcccgttt
     1441 actccggctc caaggcggcg gtggttaact ttacctcctc cctggcggta agtaatccaa
     1501 cgtttttcct actccccaaa actaaatatt tctttgaaat cccatagaaa ctggctccca
     1561 ttactggtgt cactgcttac actgtcaatc ctggcatcac taagaccact ctggtccaca
     1621 aattcaactc gtggctggat gtggagcccc gtgtggcaga gaagctgctc gagcatccca
     1681 cccagacctc tcagcagtgc gccgagaact ttgtgaaggc cattgagctg aacaagaacg
     1741 gtgccatctg gaagttggat ttgggcacct tggagcccat cacatggacc cagcactggg
     1801 actcgggcat ctaaaccatc ccagagactc tatgggacat ggcgttttag cttttagttt
     1861 ctttttttcc actcaattgt tacgtatata catacacata tggcaataag gctgatttga
     1921 accccgtttt gaatatgata atacaaaatt atatttgaga aatttcaaca aaatcgataa
     1981 gagctatagc tctcttttaa ttaaaaaaaa ctagataaaa agagcaatgg tcagtggtgg
     2041 tcggtggtcg ttgtggcctc tatcgatttc cacacaaaaa ctttactgtt tagtaataga
     2101 aaagaacttg agaaaggcag ccaaaagaat gtacgatctg acgggtaagc atgtctgcta
     2161 tgtggctgac tgcggtggca ttgcactgga gaccagcaag gttctcatga ccaagaatat
     2221 agcggtgagt ggtagagtgg tgtagagagt ggagagtggt tctggttctt aaacaagtct
     2281 ctagtctctt ggctgctgct ggtagctgtc gaaactaatt aagttaatga ctttttcatg
     2341 ttttgttttt gtccgctgac ttttgctgtt caaagctaca attaagttta tgttttgagt
     2401 ttgatctact tttgcttaag ggaagtactt tcgattacgg attgctggca tataaaaata
     2461 gataccaagg aatgtgcatc atattgattc tatcttgatt ctttgcccag aaactggcca
     2521 tccttcagag cgtggaaaac cccccggcca tcgctcagtt acaatcgatt aagcacagca
     2581 cacagatctt cttctggacc ttcgatgtga ccatggcccg cgaggagatg aagaagtact
     2641 tcgatgaggt catggtccag atggactaca tcgatgtact gatcaatggg gccaccctgt
     2701 gcgatgagcg gaacattgat gccaccatca atacgaatct gaccggcatg atgaacaccg
     2761 tggccactgt gctgccctat atggaccgaa agatgggcgg ttcgggtgga ctgattgtga
     2821 atgtcacctc cgtcatagga ctggatccat cgccagtctt ttgtgcgtac agtgcctcaa
     2881 agtttggtgt gattgggttc acccgaagtc tagcggtgag tgggcgagat cttaacttga
     2941 aaatgctctc taatgtcttc cattctttcg caggatcccc tgtattacac ccaaaatggt
     3001 gtggctgtca tggccgtctg ctgtggtccc accaaagtgt ttgtcgatcg ggaactgact
     3061 gccttcctgg aatacggcca aacctttgcc gatcgcctgc gtcgtgcccc ctgccaatcg
     3121 acggccgtct gcggccagaa tatagtaaat gccatcgaga gatcggagaa cggacagatc
     3181 tggattgccg acaagggcgg cctggagtcg gtggcccttc actggtattg gcatatggcc
     3241 gatcagtttg tgaactatat gcagagcact gacgatgagg atcaggagtt ttttttgggc
     3301 cagcgatgag gcaagccttg atccacggaa taaaacatca aatgtgtcac aaaaagtagg
     3361 caacttgttt gtgggtctgg gaatagattt gaaatatttt gaggtcatat gaataatgac
     3421 ataaatgagg gtgcctgaaa gtatgccaag ttt
//