From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: H.sapiens hTOP1 gene for topoisomerase, 5'end
Date: 10 Apr 92 10:06:50 GMT
Distribution: bionet
Checksum: 62946     6

LOCUS       HUMTOP1A     1684 bp ds-DNA             PRI       10-APR-1992
DEFINITION  H.sapiens hTOP1 gene for topoisomerase, 5'end
ACCESSION   X52601
KEYWORDS    top1 gene; topoisomerase I.
SOURCE      Homo sapiens DNA.
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 1684)
  AUTHORS   Kunze,N., Klein,M., Richter,A. and Knippers,R.
  TITLE     Structural characterization of the human DNA topoisomerase I gene
            promoter.
  JOURNAL   Eur. J. Biochem. 194, 323-330 (1990)
  STANDARD  full automatic
COMMENT     *source: cell_type=lymphocyte; *source: clone_library=lambda L47.1;
            *source: clone=J7.1;
            From EMBL    entry HSTOP15;  dated 12-FEB-1992.
FEATURES             Location/Qualifiers
     CDS             join(1090..1122,1433..1457)
                     /partial
                     /product="topoisomerase I"
                     /gene="TOP1"
                     /codon_start=1
                     /translation="MSGDHLHNDSQIEADFRLN"
                     /partial
     misc_binding    676..683
                     /bound_moiety="transcription-factor Oct1"
     misc_binding    722..729
                     /bound_moiety="transcription-factor Oct1"
     misc_feature    793..800
                     /note="cAMP responsive element"
     exon            (843.845)..1122
                     /number=1
                     /note="alternative start sites"
                     /gene="TOP1"
     exon            847..1122
                     /number=1
                     /note="alternative start site"
                     /gene="TOP1"
                     /evidence=EXPERIMENTAL
     exon            849..1122
                     /number=1
                     /note="alternative start site"
                     /gene="TOP1"
                     /evidence=EXPERIMENTAL
     exon            (851.852)..1122
                     /number=1
                     /note="alternative start sites"
                     /gene="TOP1"
                     /evidence=EXPERIMENTAL
     exon            856..1122
                     /number=1
                     /note="alternative start site"
                     /gene="TOP1"
                     /evidence=EXPERIMENTAL
     exon            879..1122
                     /number=1
                     /note="alternative start site"
                     /gene="TOP1"
                     /evidence=EXPERIMENTAL
     exon            881..1122
                     /number=1
                     /note="alternative start site"
                     /gene="TOP1"
                     /evidence=EXPERIMENTAL
     exon            883..1122
                     /number=1
                     /note="alternative start site"
                     /gene="TOP1"
                     /evidence=EXPERIMENTAL
     intron          1123..1432
                     /number=1
                     /gene="TOP1"
     exon            1433..1457
                     /number=2
                     /gene="TOP1"
     intron          1458..>1459
                     /number=2
                     /gene="TOP1"
BASE COUNT      306 a    536 c    539 g    303 t
ORIGIN
        1 gaattcagag ctgggcacag aatagatgct ctataaacgg tagttctatt ccccatttgc
       61 cccgtcctcc cagcatgcca gggatacagg ggcggataga gacaggttca gttccctttg
      121 gtctcttgca ttcccccttc cctcagcaca attcctgggc tcatagcgga tgctcaataa
      181 gtatttgtag aatgaaccaa agaaatcaga cccttggtga cccagggcta catgtactcg
      241 gcggaaaact ggggttaaac cggacccttg gttggttgaa aaattaaaat aatttttaaa
      301 aagtgggcgt gaaataatcc agaagttgga gggcctggac aaaggctggg gaggggactc
      361 ctcccaccag gcggcctcag ggtcggctta gcaagaaagg aggcgctggg gagacctctg
      421 gggtcccagc tgccaaggta ctcgccgctc caaggagatg cggacgccgg ataccagctc
      481 tgcccaagat tgctgggtcc cgccgcggcc tttaaaggcc caccacgtgg ctgcgcggcc
      541 agccctggtc cggaggcggt gcccgggtgg cgcctgcgca ctgcctagca gaggggctgg
      601 ggttccgaga aaaagcgtct ggagaggaag gggaggctgc ttggggtggg acgccgtggg
      661 aggagtcggc tccttatgca aatcacagcg gagcgcgcac ggtccggagg cggggcttgc
      721 gatgcaaaga caggtccgtc tggcgaacag cgagggggcg ggccgcaacc ctctgcctct
      781 ttccgcgagc gctgacgtcg ccgacgtgtt gtttaaaagc ggccgcgcag gcgcagtgag
      841 cccaaatgcg aacttaggct gttacacaac tgctggggtc tgttctcgcc gcccgcccgg
      901 cagtcaggca gcgtcgccgc cgtggtagca gcctcagccg tttctggagt ctcgggccca
      961 cagtcaccgc cgcttacctg cgcctcctcg agcctccgga gtccccgtcc cgcccgcaca
     1021 aggccggtcg ccgtctgcgt ctcccccacg ccgcctcgcc tgccgccgcg ctcgtccctc
     1081 cgggccgaca tgagtgggga ccacctccac aacgattccc aggtacggcc caacctgacc
     1141 ctggcggccc cgaccccggc ctggccgtcc gcgacccccg gacgcaggcc ccgaccccag
     1201 ccccggcccg gcagctttac aggccggagc ccccggtgag gggccgcctg ccggagtaga
     1261 tcggctcgct aggccgcagc gagggccgcg aagttacagt tcgaggcagg gatggctgcc
     1321 ctctgtggcc acccccgggg gcgcagggtg agccagaccc cggccgcgcg cgctcgccgc
     1381 cggaggggtt aaagtggctg ttgtttgata ttctctcctt ttctttttcc agatcgaagc
     1441 ggatttccga ttgaatggtg agtgtgccct gcgccgactc cggcccagcg caccggctcc
     1501 cgcgcctgcg tgcggcagag gacagactag gcgtccagag actagtccgg ctctcgctca
     1561 cggcccattg ttccatcggc gcttgaccct tcggaccagt ctcagatcgc tcaagaggac
     1621 aacggagacg tgtgtcgcac ggtggcgtgg attgatctta gagtgactag agtgcactgc
     1681 actg
//