From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Allium cepa alliinase (Alli) mRNA, complete cds.
Date: 29 Dec 92 19:56:34 GMT
Distribution: bionet
Checksum: 29623 5

LOCUS       ALCALLI      1740 bp ss-mRNA            PLN       10-DEC-1992
DEFINITION  Allium cepa alliinase (Alli) mRNA, complete cds.
ACCESSION   M98267
KEYWORDS    alliin lyase; alliinase.
SOURCE      Allium cepa (cultivar Southport White Globe) mature bulb and
            sprouting foliage leaf cDNA to mRNA.
  ORGANISM  Allium cepa
            Eukaryota; Plantae; Embryobionta; Magnoliophyta; Liliopsida;
            Liliidae; Liliales; Liliaceae.
REFERENCE   1  (bases 1 to 1740)
  AUTHORS   Clark,S.A., Shaw,M.L., Leung,D.W. and Lancaster,J.E.
  TITLE     Isolation and characterization of a cDNA clone encoding alliinase
            -the enzyme responsible for flavour release in onions
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     sig_peptide     138..239
                     /gene="Alli"
                     /codon_start=1
     mat_peptide     240..1574
                     /product="alliinase"
                     /gene="Alli"
                     /codon_start=1
     polyA_signal    1671..1676
                     /gene="Alli"
                     /note="putative"
     polyA_signal    1675..1680
                     /gene="Alli"
                     /note="putative"
     polyA_signal    1720..1726
                     /gene="Alli"
                     /note="putative"
     polyA_site      1740
                     /gene="Alli"
     CDS             138..1577
                     /gene="Alli"
                     /product="alliinase"
                     /codon_start=1
                     /translation="MESYHKVGSNKMPSLLILICIIMSSFVNNNIAQAKVTWSLKAAE
                     EAEAVANINCSGHGRAFLDGILSDGSPKCECNTCYTGADCSEKITGCSADVASGDGLF
                     LEEYWQQHKENSAVLVSGWHRMSYFFNPVSNFISFELEKTIKELHEIVGNAAAKDRYI
                     VFGVGVTQLIHGLVISLSPNMTATPCAPQSKVVAHAPYYPVFREQTKYFDKKGYEWKG
                     NAADYVNTSTPEQFIEMVTSPNNPEGLLRHEVIKGCKSIYDMVYYWPHYTPIKYKADE
                     DIMLFTMSKYTGHSGSRFGWALIKDETVYNKLLNYMTKNTEGTSRETQLRSLKILKEV
                     IAMVKTQKGTMRDLNTFGFQKLRERWVNITSLLDKSDRFSYQKLPQSEYCNYFRRMRP
                     PSPSYAWVKCEWEEDKDCYQTFQNGRINTQNGEGFEAGSRYVRLSLIKTKDDFDQLMY
                     YLKNMVEAKRKTPLIKQLSNDQISRRPFI"
BASE COUNT      551 a    321 c    369 g    499 t
ORIGIN      
        1 attttggttt gatgaaatat gtaggctgcc tcactttcta tttacccatg tctgttaatt
       61 ccatgttgcg gaatggaaca tacctcattt caaaacattt tcagtaagcg agatatccat
      121 agcagagcta attagctatg gagtcttacc acaaagttgg cagtaataaa atgccaagcc
      181 ttcttatttt gatatgcata atcatgtctt catttgttaa caataatata gctcaagcga
      241 aggtgacatg gagtttgaag gcagcagaag aggcagaagc agtggctaat ataaactgtt
      301 cagggcatgg gagagctttt ttggacggaa ttctttcaga tggatctcct aaatgcgagt
      361 gcaatacttg ctacactggt gcagactgct ctgaaaagat tacaggttgc tctgctgatg
      421 ttgccagtgg tgatggactg tttctagaag aatactggca gcagcacaag gaaaacagtg
      481 cagtgctggt ttcaggatgg cacagaatga gctacttttt caacccagtt agcaatttca
      541 tatctttcga gcttgaaaaa acaattaaag aactacatga gatagtcgga aatgctgctg
      601 caaaggacag gtacattgtg tttggagtag gggtgactca actcatccat ggattggtca
      661 tctccctttc accaaatatg actgccactc cttgtgcacc acaatctaaa gttgttgctc
      721 atgcccctta ttatccggtg tttagagaac aaacaaagta ctttgacaag aaagggtacg
      781 agtggaaagg aaatgcagcg gattacgtga acacttcaac tccagagcaa ttcattgaga
      841 tggttacttc acctaataac ccagaaggtc tgcttcgcca tgaagtaata aagggatgca
      901 aatccatcta cgatatggtt tactactggc ctcattacac cccaatcaag tacaaagccg
      961 atgaagatat catgttgttt acaatgtcta aatacactgg acactctggt agtcgatttg
     1021 ggtgggcact gataaaggat gaaactgtgt ataataaatt gttgaattac atgacaaaga
     1081 acacggaggg cacttcccga gaaacacagc tacgatcgct caaaattcta aaagaagtta
     1141 tagcaatggt taaaacacag aaaggcacca tgcgcgacct caacacattt ggttttcaga
     1201 aactaagaga gaggtgggta aatatcactt cattgctcga taaatccgac agattctcct
     1261 atcaaaagct tccacaaagt gaatattgca attacttcag gagaatgaga cctccatccc
     1321 catcttatgc atgggtgaag tgtgaatggg aagaagacaa agattgctac cagacatttc
     1381 agaatgggcg tatcaatacg caaaatggag agggtttcga agcaggtagt cgttatgtgc
     1441 gtttgagttt gatcaagaca aaagatgatt ttgatcaact aatgtactat ttgaagaata
     1501 tggttgaagc aaagaggaag actcctctca tcaaacaact ttccaatgat cagatttccc
     1561 gccgtccttt catttaagta ctcatgttat gtattgctct gctgttttgt tagtgtatga
     1621 ctatgttcat acatcctaat gctatggtag taaggagtat ctttctatgc aataaataaa
     1681 gttcatgttt gtgatcatgt atgggctact atgattttat aataaaatca attttcatat
//