From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: sCos-1 cosmid cloning vector.
Date: 4 Sep 92 10:07:13 GMT
Distribution: bionet
Checksum: 14915    11

LOCUS       SYNSCOS      7937 bp ds-DNA             SYN       04-SEP-1992
DEFINITION  sCos-1 cosmid cloning vector.
ACCESSION   M99566
KEYWORDS    cloning vector.
SOURCE      Cloning vector DNA.
  ORGANISM  Cloning vector
            Artificial sequences; Cloning vehicles.
REFERENCE   1  (sites)
  AUTHORS   Evans,G.A., Lewis,K. and Rothenberg,B.E.
  TITLE     High efficiency vectors for cosmid microcloning and genomic
            analysis
  JOURNAL   Gene 79, 9-20 (1989)
  STANDARD  full staff_review
REFERENCE   2  (sites)
  AUTHORS   Evans,G.A. and Lewis,K.
  TITLE     Physical mapping of complex genomes by cosmid multiplex analysis
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 86, 5030-5034 (1989)
  STANDARD  full staff_review
REFERENCE   3  (bases 1 to 7937)
  AUTHORS   Evans,G.A.
  JOURNAL   Unpublished (1992)
  STANDARD  simple automatic
BASE COUNT     1949 a   2033 c   1988 g   1967 t
ORIGIN
        1 gaattcgcgg ccgcaattaa ccctcactaa aggatcccta tagtgagtcg tattatgcgg
       61 ccgcgaattc tcatgtttga ccgcttatca tcgataagct ctgctttttg ttgacttcca
      121 ttgttcattc cacggacaaa aacagagaaa ggaaacgaca gaggccaaaa agctcgcttt
      181 cagcacctgt cgtttccttt cttttcagag ggtattttaa ataaaaacat taagttatga
      241 cgaagaagaa cggaaacgcc ttaaaccgga aaattttcat aaatagcgaa aacccgcgag
      301 gtcgccgccc cgtaacaagg cggatcgccg gaaaggaccc gcaaatgata ataattatca
      361 attgcatact atcgacggca ctgctgccag ataacaccac cggggaaaca ttccatcatg
      421 atggccgtgc ggacatagga agccagttca tccatcgctt tcttgtctgc tgccatttgc
      481 tttgtgacat ccagcgccgc acattcagca gcgtttttca gcgcgttttc gatcaacgtt
      541 tcaatgttgg tatcaacacc aggtttaact ttgaacttat cggcactgac ggttaccttg
      601 ttctgcgctg gctcatcacg caggatacca aggctgatgt tgtagatatt ggtcaccggc
      661 tgagggtttt cgattgccgc tgcgtggata gcaccatttg cgatcaggcg tccttgatga
      721 atgacactcc attgcgaata agttcgaagg agacggtgtc acgaatgcgc tggtccagct
      781 cggtcgattg ccttttgtgc agcagaggta tcaatctcaa cgccaaggct catcgaagcg
      841 caatattgct gctcaccaaa acgcgtattg accaggtgtt caacggcaaa tttctgccct
      901 tctgatgtca gaaaggcaaa gtgattttct ttctggtatt cagttgctgt gtgtcggttt
      961 cagcaaaacc aagctcgcgc aattcggctg tgcagattta gaaggcagat caccagacag
     1021 caacggccaa cggaaaacag cgcatacaga acatccgtcg ccgcgccgac aacgtgataa
     1081 tttttatgac ccatgattta tttcctttta gacgtgagcc tgtcgcacag caaagccgcc
     1141 gaaagttcct cgaagctagc ttcagacgtg tctagatacg tctgcttttt gttgacttcc
     1201 attgttcatt ccacggacaa aaacagagaa aggaaacgac agaggccaaa aagctcgctt
     1261 tcagcacctg tcgtttcctt tcttttcaga gggtatttta aataaaaaca ttaagttatg
     1321 acgaagaaga acggaaacgc cttaaaccgg aaaattttca taaatagcga aaacccgcga
     1381 ggtcgccgcc ccgtaacaag gcggatcgcc ggaaaggacc cgcaaatgat aataattatc
     1441 aattgcatac tatcgacggc actgctgcca gataacacca ccggggaaac attccatcat
     1501 gatggccgtg cggacatagg aagccagttc atccatcgct ttcttgtctg ctgccatttg
     1561 ctttgtgaca tccagcgccg cacattcagc agcgtttttc agcgcgtttt cgatcaacgt
     1621 ttcaatgttg gtatcaacac caggtttaac tttgaactta tcggcactga cggttacctt
     1681 gttctgcgct ggctcatcac gcaggatacc aaggctgatg ttgtagatat tggtcaccgg
     1741 ctgagggttt tcgattgccg ctgcgtggat agcaccattt gcgatcaggc gtccttgatg
     1801 aatgacactc cattgcgaat aagttcgaag gagacggtgt cacgaatgcg ctggtccagc
     1861 tcggtcgatt gccttttgtg cagcagaggt atcaatctca acgccaaggc tcatcgaagc
     1921 gcaatattgc tgctcaccaa aacgcgtatt gaccaggtgt tcaacggcaa atttctgccc
     1981 ttctgatgtc agaaaggcaa agtgattttc tttctggtat tcagttgctg tgtgtcggtt
     2041 tcagcaaaac caagctcgcg caattcggct gtgcagattt agaaggcaga tcaccagaca
     2101 gcaacggcca acggaaaaca gcgcatacag aacatccgtc gccgcgccga caacgtgata
     2161 atttttatga cccatgattt atttcctttt agacgtgagc ctgtcgcaca gcaaagccgc
     2221 cgaaagttcc tcgaccgatg cccttgagag ccttcaaccc agtcagctcc ttccggtggg
     2281 cgcggggcat gactatcgtc gccgcactta tgactgtctt ctttatcatg caactcgtag
     2341 gacaggtgcc ggcagcgctc tgggtcattt tcggcgagga ccgctttcgc tggagcgcga
     2401 cgatgatcgg cctgtcgctt gcggtattcg gaatcttgca cgccctcgct caagccttcg
     2461 tcactggtcc cgccaccaaa cgtttcggcg agaagcaggc cattatcgcc ggcatggcgg
     2521 ccgacgcgct gggctacgtc ttgctggcgt tcgcgacgcg aggctggatg gccttcccca
     2581 ttatgattct tctcgcttcc ggcggcatcg ggatgcccgc gttgcaggcc atgctgtcca
     2641 ggcaggtaga tgacgaccat cagggacagc ttcaaggatc gctcgcggct cttaccagcc
     2701 taacttcgat cattggaccg ctgatcgtca cggcgattta tgccgcctcg gcgagcacat
     2761 ggaacgggtt ggcatggatt gtaggcgccg ccctatacct tgtctgcctc cccgcgttgc
     2821 gtcgcggtgc atggagccgg gccacctcga cctgaatgga agccggcggc acctcgctaa
     2881 cggattcacc actccaagaa ttggagccaa tcaattcttg cggagaactg tgaatgcgca
     2941 aaccaaccct tggcagaaca tatccatcgc gtccgccatc tccagcagcc gcacgcggcg
     3001 catctcgggc agcgttgggt cctgcagatc cggctgtgga atgtgtgtca gttagggtgt
     3061 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     3121 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     3181 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg
     3241 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     3301 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     3361 ggcttttgca aaaagcttca cgctgccgca agcactcagg gcgcaagggc tgctaaagga
     3421 agcggaacac gtagaaagcc agtccgcaga aacggtgctg accccggatg aatgtcagct
     3481 actgggctat ctggacaagg gaaaacgcaa gcgcaaagag aaagcaggta gcttgcagtg
     3541 ggcttacatg gcgatagcta gactgggcgg ttttatggac agcaagcgaa ccggaattgc
     3601 cagctggggc gccctctggt aaggttggga agccctgcaa agtaaactgg atggctttct
     3661 tgccgccaag gatctgatgg cgcaggggat caagatctga tcaagagaca ggatgaggat
     3721 cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga
     3781 ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc
     3841 ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga
     3901 atgaactgca ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg
     3961 cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc
     4021 cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg
     4081 atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga
     4141 aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc
     4201 tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca
     4261 tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg
     4321 tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct
     4381 atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg
     4441 accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc
     4501 gccttcttga cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac
     4561 gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt
     4621 cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga
     4681 gttcttcgcc caccccgggc tcgatcccct cgcgagttgg ttcagctgct gcctgaggct
     4741 ggacgacctc gcggagttct accggcagtg caaatccgtc ggcatccagg aaaccagcag
     4801 cggctatccg cgcatccatg cccccgaact gcaggagtgg ggaggcacga tggccgcttt
     4861 ggtccggatc tttgtgaagg aaccttactt ctgtggtgtg acataattgg acaaactacc
     4921 tacagagatt taaagctcta aggtaaatat aaaattttta agtgtataat gtgttaaact
     4981 actgattcta attgtttgtg tattttagat tccaacctat ggaactgatg aatgggagca
     5041 gtggtggaat gcctttaatg aggaaaacct gttttgctca gaagaaatgc catctagtga
     5101 tgatgaggct actgctgact ctcaacattc tactcctcca aaaaagaaga gaaaggtaga
     5161 agaccccaag gactttcctt cagaattgct aagttttttg agtcatgctg tgtttagtaa
     5221 tagaactctt gcttgctttg ctatttacac cacaaaggaa aaagctgcac tgctatacaa
     5281 gaaaattatg gaaaaatatt ctgtaacctt tataagtagg cataacagtt ataatcataa
     5341 catactgttt tttcttactc cacacaggca tagagtgtct gctattaata actatgctca
     5401 aaaattgtgt acctttagct ttttaatttg taaaggggtt aataaggaat atttgatgta
     5461 tagtgccttg actagagatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt
     5521 taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg
     5581 ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     5641 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     5701 cttatcatgt ctggatctga cgggtgcgca tgatcgtgct cctgtcgttg aggacccggc
     5761 taggctggcg gggttgcctt actggttagc agaatgaatc accgatacgc gagcgaacgt
     5821 gaagcgactg ctgctgcaaa acgtctgcga cctgagcaac aacatgaatg gtcttcggtt
     5881 tccgtgtttc gtaaagtctg gaaacgcgga agtcagcgct cttccgcttc ctcgctcact
     5941 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     6001 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     6061 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     6121 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     6181 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     6241 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     6301 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     6361 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     6421 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     6481 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     6541 gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     6601 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     6661 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     6721 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     6781 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     6841 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     6901 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     6961 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     7021 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     7081 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     7141 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctgca ggcatcgtgg
     7201 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     7261 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     7321 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     7381 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     7441 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaaca cgggataata
     7501 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     7561 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     7621 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     7681 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     7741 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     7801 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     7861 ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga
     7921 ggccctttcg tcttcaa
//