From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: E. coli DNA helicase II (uvrD) gene, 3' end; Mg-transport system I
Date: 8 Jan 93 05:46:57 GMT
Distribution: bionet
Checksum: 21300 12

LOCUS       ECOCORAX     4722 bp ds-DNA             BCT       07-JAN-1993
DEFINITION  E. coli DNA helicase II (uvrD) gene, 3' end; Mg-transport system I
            (corA) gene, complete cds; chloamphenicol sensitive (rarD) gene,
            complete cds; detergent-resistant phospholipase A (pldA) gene, 5'
            end.
ACCESSION   L02122
KEYWORDS    DNA helicase II; Mg-transport system I;
            chloramphenicol sensitivity; corA gene;
            detergent-resistant phospholipase A; pldA gene; rarD gene;
            uvrD gene.
SOURCE      Escherichia coli (individual_isolate , strain K-12) DNA.
  ORGANISM  Escherichia coli
            Prokaryotae; Gram-negative facultatively anaerobic rods;
            Enterobacteriaceae.
REFERENCE   1  (bases 1 to 4722)
  AUTHORS   Ohmori,H.
  TITLE     Physical map of the corA region of the E.coli chromosome
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
REFERENCE   2  (bases 1 to 4722)
  AUTHORS   Daniels,D.L., Plunkett,G.III., Burland,V. and Blattner,F.R.
  TITLE     Analysis of the Escherichia coli Genome:DNA Sequence of the Region
            from 84.5 to 86.5 Minutes
  JOURNAL   Science 257, 771-778 (1992)
  STANDARD  full automatic
REFERENCE   3  (sites)
  AUTHORS   Daniels,D.L., Plunkett,G.III., Burland,V.D. and Blattner,F.R.
  TITLE     Analysis of the Escherichia coli genome: DNA Sequence of the region
            from 84.5 to 86.5 minutes
  JOURNAL   Science 257, 771-778 (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     misc_feature    1..3
                     /note="This feature applies to a gene which lacks a coding
                     region feature. "
                     /gene="uvrD"
     CDS             <1..3
                     /product="DNA helicase II"
                     /gene="uvrD"
                     /partial
                     /note="termination codon of uvrD"
     conflict        replace(535..536,"g")
                     /citation=[2]
                     /citation=[2]
     conflict        replace(1162..1163,"g")
                     /citation=[2]
                     /citation=[2]
     conflict        replace(1430..1433,"gcct")
                     /citation=[2]
                     /citation=[2]
     conflict        replace(2183..2184,"c")
                     /citation=[2]
                     /citation=[2]
     terminator      2246..2270
                     /note="terminator for transcriptions from both directions"
     attenuator      complement(3091..3124)
                     /note="terminator-like sequence"
     conflict        replace(3667..3678,"cg")
                     /citation=[2]
                     /citation=[2]
     conflict        replace(3732..3733,"cg")
                     /citation=[2]
                     /citation=[2]
     conflict        replace(3963..3965,"gt")
                     /citation=[2]
                     /citation=[2]
     conflict        replace(4270..4271,"cg")
                     /citation=[2]
                     /citation=[2]
     CDS             complement(150..914)
                     /function="unknown"
                     /codon_start=1
                     /translation="MAHQLLIGKGMITLNLKRIFLALTLLPLFAVAADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADINSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQKVALNLASGEGNFFIRPGGVFYVAGDKVGIVRLDAFKTSKEIQFAVQSGP
                     MLMENGVINPRIHPNVASSKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKAKLNVE
                     QLLYLDGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     CDS             1284..2234
                     /function="Mg-transport system I"
                     /gene="corA"
                     /codon_start=1
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     CDS             complement(2277..2657)
                     /function="unknown"
                     /codon_start=1
                     /translation="MSKEYMNDGSLSEKWKYRFNFYDQHGFPGFWGATPEYKAAFKAL
                     KVRQRLTIQMNFIAFFCSWIYLFVLGLWKKAIIVLLLGILSLFVGALIGVNILGIAVA
                     AYVAVNTNKWFYEKEVKGLNTWSL"
     CDS             complement(2671..3087)
                     /function="unknown"
                     /codon_start=1
                     /translation="MLTGDSYKDVKFMLRIFIPTSNGKISRRRYIFSFILINFIFAFL
                     IIFFNDGEAGFLVIVSTIVLHYLVINMNCQRLRDSGFIYIKTYVFGTLAVYIISIITM
                     IAEDFACSGNGSMIFLICYFSTFSMLMLAPTDSSKQ"
     CDS             complement(3146..4036)
                     /gene="rarD"
                     /codon_start=1
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFAFI
                     WVALAIFVMDAIYTQRRTSK"
     CDS             complement(4088..4573)
                     /function="unknown"
                     /codon_start=1
                     /translation="MKCKDDMSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKE
                     FAQLAFKNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRL
                     SRMGTIDLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMV
                     G"
     CDS             4720..4722
                     /gene="pldA"
                     /product="detergent-resistant phospholipase A"
                     /codon_start=1
                     /translation="M"
BASE COUNT     1304 a   1146 c   1062 g   1210 t
ORIGIN      
        1 taacgttgcc ggatgcggtg ctgcgcacct tatttggcct aaaaaatcat tcagattcaa
       61 taaattgcaa cgtcatgtag gccggatagg gcgtttacgc cgcatccggc atctgcgcca
      121 tcttcagtat ctgacacaaa actatcgttt taacctttcc gctcgacgga aatcatggtg
      181 acaaaaggat agcgttgcca cgggattgcc ccgcctttca tatacatatg tgaaatcgtg
      241 ccatcaagat aaagcagctg ctcaacgttc agtttcgctt tggcataaca ggcaaaatca
      301 taaaaatttg ttgcctgctg gctcaacaaa aacacggcgt tcccatgttt attaatccca
      361 acaccgttac gaattttgct tgaggcgacg ttgggatgaa tacgcggatt aattacaccg
      421 ttttccatca acattggccc tgactgcacc gcaaactgaa tctctttact ggttttgaag
      481 gcatccagac gaacgatgcc gactttatct cccgcgacat aaaacacgcc gccaggacgg
      541 ataaagaaat tcccttcacc tgaagcgaga tttaacgcca ccttctgctg accgttttcg
      601 atgtacaaac cgagcggcgc atagctttca tcatagatgc cgccgttcat cgccatctgc
      661 acctgaccct gactattaat atccgccagc agagcatgta acgttcccca cgcttcgcca
      721 ttggcttttt gccagtacat tttcacccgc tctgtttgag gattaacggt atacgcctgt
      781 acggtcagcg tcggatctga gagtgcgcaa tcatcagcgg caacagcaaa caagggaagt
      841 aaggtgaggg cgagaaaaat ccgtttgaga ttcaaggtga tcattccttt accaatgagt
      901 agctgatgcg ccattatagg tcctggatgt gggatttttt tatcctgtta gcgaccttga
      961 cgagtaccaa aaagcgcgaa gttcaactat tgttctgtgg tgttctgttg cgtgttgacg
     1021 gcaaaatttt gctggcgtaa catgcgcgca cgatcactct aagaggacat tcgccttgga
     1081 cacacccagt agatactggc tcactatcct gtcatccagg atcaactcct aaggctatcc
     1141 ctttttgctg atagccttag cggttgtcag cgacctcaat ttttcccgtc gcgctgagtc
     1201 aggctgttta atggtctgaa acccaatttg tttctgtgtg cccaccgaac tgtccgatat
     1261 tttaagcatt gggagtcccg gtcatgctga gcgcatttca actggaaaat aaccgactga
     1321 cccggctgga agtcgaagag tcacaacccc ttgtaaatgc agtatggatt gatcttgtcg
     1381 aaccggacga cgacgagcga ctgcgcgtac aatctgaact tggccagagc ctggcaaccc
     1441 gcccggaact ggaagacatc gaagcatcgg cacgtttctt tgaagacgac gacggcctgc
     1501 atattcactc cttcttcttc tttgaagatg cggaagatca cgccggtaac tccactgtgg
     1561 catttaccat ccgtgatggt cgtctgttta ctctgcgtga gcgtgaactg cccgcttttc
     1621 gtctgtatcg tatgcgtgcc cgtagccagt cgatggtaga cggtaacgcc tacgagttgc
     1681 tgctggatct gttcgaaacc aaaatcgaac agttggcaga tgaaattgaa aatatctata
     1741 gcgacctgga gcagttgagc cgggtgatta tggaagggca tcagggcgat gagtacgacg
     1801 aggcgctctc cactctggcg gaactggaag atatcggctg gaaagttcgc ctgtgtctga
     1861 tggataccca gcgcgcgctc aacttcctgg tgcgtaaagc gcgtttaccg ggtgggcaac
     1921 tggagcaggc gcgtgaaatc ctgcgagata tcgaatccct gctgccgcat aacgaatccc
     1981 tgttccagaa ggtgaacttc ctgatgcagg cggcaatggg ttttatcaac atcgagcaga
     2041 accgcatcat caaaatcttc tcggtggtat ccgtggtatt cctgccgccg acgctcgttg
     2101 cttccagcta tggcatgaac tttgagttta tgccagaact gaagtggagc ttcggctacc
     2161 ctggcgcgat tatctttatg atcctcgcgg gcctggcacc gtatctgtac tttaagcgga
     2221 agaactggtt gtaaaaaacg agagcggtgg cttagtctgg ctaagccacc tgttattcaa
     2281 aggctccagg tatttaaccc ttttacctct ttctcataga accatttgtt cgtgttaaca
     2341 gcaacatagg ctgctacggc aattcccaga atgttaacgc caattagtgc accaacaaat
     2401 agagatagaa taccaagtaa tagaactata attgcttttt tccataaccc caggacaaat
     2461 aaatatatcc aggaacagaa gaaagcaatg aaattcattt gaatagttaa gcgttgtctt
     2521 acttttaatg ctttaaatgc tgctttatat tctggtgttg ccccccagaa tccaggaaaa
     2581 ccatgttgat cataaaaatt aaatcggtat ttccattttt cacttaatga accatcgttc
     2641 atatattcct tactcataaa tactccataa ctattgtttt gatgaatcag taggtgcaag
     2701 cattagcata ctgaaagtgg aaaaataaca aatcaaaaaa atcatcgaac cattgcctga
     2761 acaggcaaaa tcttcggcta tcattgtgat gatagagatg atatatactg ctaatgtacc
     2821 aaaaacataa gtttttatat agatgaaacc actatcacgg agtcgctggc aattcatgtt
     2881 gatgacgaga taatggagta cgatggtaga gactataaca agaaagcctg cttctccatc
     2941 gttaaaaaag ataataagaa aggcaaaaat gaaatttatt aaaataaatg aaaatatata
     3001 acgacgtctg gaaatcttac cgttagatgt tgggataaat atacgtaaca taaattttac
     3061 atccttgtat gagtctccgg tcagcatggc aatatgccca ctctcatgca agagtcggca
     3121 tattttttca gaatatattt attttttatt tggacgttct acgctgcgta taaatcgcat
     3181 ccatcacaaa aattgccagc gccacccaaa taaaggcgaa agtcaccatc ttatcggcac
     3241 ccggtttttc accataaaac gtcacagcca gcaggaacat cagcgtcggg ccaatgtact
     3301 ggaaaaagcc taacgttgag agacgcaagc gcgtggcagc ggcggtaaaa cacaacagcg
     3361 gtacggtagt gacaataccg gcggcgatca gcagtaaatt cagcgacatc gggttttgcc
     3421 ccatatggct ggttgagctg tcggcaatag caaacaggta aattgccgcc acgggcagca
     3481 gccacatggt ttcgattaac atgccggttt gcgcttcaac ggcaatcttc ttgcgtacca
     3541 gaccgtagaa ggcaaaacta aatgccagtc ccagcgcgat aataggtagc gaaccaaaag
     3601 tccacagctg gactaacacg ccacatatcg ccagaatcac cgccagccat tgcatccggc
     3661 ggaatcgctc gccgaggaaa atcatcccca gcacaatgtt caccagcggg ttaataaagt
     3721 aaccaaggct cgcttccagc atatggtgat tgttcaccgc ccagataaac agtagccagt
     3781 tgccaccaat cagcacggca gagactgcca gcataaaaat tttctgtggc gtctgaatca
     3841 gcgtttttaa ataggaccac tggcggcaaa tgctcatcag caccaccata aagaaaaacg
     3901 accagatcac gcgatgcgtc aggatttcat cggcgggcac gtagtaaatc aacttgaagt
     3961 acgctggcgc tataccccaa ataaaataag cggcaagagc gagtaatacg ccctgccgcg
     4021 tttgttttgc atccatcggg aatactcatt tttaattggt aacagcagtt tacctgcttt
     4081 tatgtcttca acctaccata taggtggcgg tggcactggc aatataaagc tgttcttcat
     4141 tgtgtaattc aacgcgggcg acggcgactt tattgcctgc acgcaacagg ctactagtag
     4201 cagtaaaacg ctcgcccctg cctgggcgca gataatcaac gcgaagatca atggtcccca
     4261 tccgcgatag ccgctggcgt agttcatctt cactgatggt ttcgtggcgg gttaaggtac
     4321 ttcccacgca caccagaccg gcggcgacat ccagcgccga cgcaatgacc ccgccgtgca
     4381 aaatgctttg cgcccagttg cccaccatca ttggctgatt tttaaaggcc agctgtgcga
     4441 actctttttc gtaacgctcc agttccatcc ccaatgcgcg gttaaatggc atgtgataaa
     4501 caaacatctc acccactaat ttcagggctt gttcagcggt cagtacggca gacatatcat
     4561 ccttacactt cattggttaa tgaaatgttg attttatgct tctttgttgt tggtttctac
     4621 tttaggaagg gataactaac ggctatggag ttaagtatgt aaaatagccc gcagaaaaat
     4681 attcacctta tcaataattc gttacggaga acacgaccga tg
//