From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Tomato spotted wilt virus L RNA encoding RNA polymerase, complete
Date: 9 Apr 92 10:05:30 GMT
Distribution: bionet
Checksum: 38780    17

LOCUS       TSWLRPOLM    8897 bp ss-RNA             VRL       09-APR-1992
DEFINITION  Tomato spotted wilt virus L RNA encoding RNA polymerase, complete
            cds.
ACCESSION   D10066 D01230
KEYWORDS    RNA polymerase.
SOURCE      Tomato spotted wilt virus (strain BR-01 (CNPH1)) cDNA to genomic
            RNA.
  ORGANISM  Tomato spotted wilt virus
            Viridae; ss-RNA enveloped viruses; Negative strand RNA viruses;
            Bunyaviridae.
REFERENCE   1  (bases 1 to 8897)
  AUTHORS   De Haan,P., Kormelink,R., Resende,R.O., Van Poelwijk,F., Peters,D.
            and Goldbach,R.
  TITLE     Tomato spotted wilt virus L RNA encodes a putative RNA polymerase
  JOURNAL   J. Gen. Virol. 72, 2207-2216 (1991)
  STANDARD  full staff_review
COMMENT     Data kindly submitted in computer readable form by:
            Peter De Haan
            Department of Virology
            Agricultural University Wageningen
            Binnenhaven 11
            6709 PD Wageningen
            P.O.Box 8045
            The Netherlands
            Phone:  +31 837083094
            Telex:  NL45015
            Fax:    +31 837084820
FEATURES             Location/Qualifiers
     CDS             34..8661
                     /product="RNA polymerase"
                     /codon_start=1
                     /translation="MNIQKIQKLIENGTTLLLSIEDCVGSNHDLALDLHKRNSDEIPE
                     DVIINNNAKNYETMRELIVKITADGEGLNKGMATVDVKKLSEMVSLFEQKYLETELAR
                     HDIFGELISRHLRIKPKQRNEVEIEHALREYLDELNKKSCINKLSDDEFERINKEYVA
                     TNATPDNYVIYKESKNSELCLIIYDWKISVDARTETKQWRNTYKNIWKSFKDIKVNGK
                     PFLEEHPVFVSIVILKPIAGMPITVTSSRVLEKFEDSPSALHGERIKHAKNAKLLNIS
                     YVGQIVGTTPTVVRNYYANTQRIKSEVRGILGDDFGSKDVFFSHWTSKYKERNPTEIA
                     YSEDIERIIDSLVTDEIPREEIIHFLFGNFCFHIETMNDQHIADKFKGYQNSCINLKI
                     EPKADLADLKDHLIQKQQIWESLYGKHLEKIMLRIREKKRKEKEIPDITTAFNQNAAE
                     YEERYPNCFNDLSELKLTFHDLVPSLKIELSSEVDYNNAIINKFRESFKSSSRVIYNS
                     PYSSINNQTNKARDITNLVRLCLAELSCDTTKMEKQELEDEIDINTGSIKVERTKKSK
                     EWNKQGSCLTRNKNEFCMKDTGRENKTTYFKGLAVMNIGMSSKKRILKKEEIKERISK
                     GLEYDTSERQADPNDDYSSIDMSSLTHMKKLIRHDNDDSLSGKRFKGSFFLLHNFNII
                     EDGKITSVFNNYAKNPECLYIQDSVLKTELETCKKINKLCNDLAIYHYSEDMMQFSKG
                     LMVADRYMTKESFKILTTANTSMMLLAFKGDGMNTGGSGVPYIALHIVDEDMSDHFNI
                     CYTKEIYSYFRSGSNYIYIMRPQRLNQVRLLRLFKTPSKVPVCFPQFSKKANEIGKSL
                     KNKDIEKVNLFSMTMTVKQILINIVFSSVMIGTVTKLSRMGIFDFMRYAGFLRLSDYS
                     NIKEYIRDKSDPDITNCGRYLFRNGIKKLLFRMEDLNLSTNAKPVVVDHENDIIGGIT
                     NLNIKCPITGSTLLTLEDLYNNVYLAIYMMPKSLHNHVHNLTSLLNLPAEWELKFRKE
                     LGFNIFEDIYPKKAMFDDKDLFSINGALNVKALSDYYLGNIENVGLMRSEIENKEDFL
                     SPCYKISTLKSSKKCSQSNIISTDEIIECLQDAKIQDIENWKGNNLAIIKGLIRTYNE
                     EKNRLVEFFEDNCVNSLYLIEKLKEIISSGSITVGKSVTSKFIRNNHPLTVETYLKTK
                     LYYRNNVTVLKSKKVSEELYDLVKQFHDMMEIDLDSVMNLGKGTEGKKLTFLQMLEFV
                     MSKAKNVTGSVDFLVSVFEKMQRTKTDREIYLMSMKVKMMLYFIEHTFKHVAQSDPSE
                     AISISGDNKIRALSTLSLDTITSYNDILNKNSKKSRLAFLSADQSKWSASGLTTYKYV
                     LAIILNPILTTGEASLMIECILMYVKLKKVCIPTDIFLNLRKAQQTFGENETAIGLLT
                     KGLTTNTYPVSMNWLQGNLNYLSSVYHSCAMKAYHNTLECYKNCDFQTRWIVHSDDNA
                     TSLIASGEVDKMLTDFSSSSLPEMLFRSIEAHFKSFCITLNPKKSYASSSEVEFISER
                     ISKWSDYSSLLQAFSKLLHRIFAYKLFDDLMSLSIHVTMLLRKGCPNEVIPFAYGAVQ
                     VQALSIYSMLPGEVNDSIRIFNKLGVSLKSNEIPTNMGGWLTSPIEPLSILGPSSNDQ
                     IIYYNVIRDFLNKKSLEEVKDSVSSSSYLQMRFRELKEKYERGTLEEKDKKMIFLINL
                     FEKASVSEDSDVLTIGMKFQTMLTQIIKLPNFINENALNKMSSYKDFSKLYPNLKKNE
                     DLYKSTKNLKIDEDAVLEEDELYKKIASSLEMESVHDIMIKNPETILIAPLNDRDFLL
                     SQLFMYTSPSKRNQLSNQSTEKLALDRVLRSKARTFVNISSTVKMTYEENMEKKILEM
                     LKFDLDSYCSFKTCVNLVIKDVNFSMLIPILDSAYPCESRKRDNYNFRWFQTERWIPV
                     VEGSPGLVVMHAVYGSNYIENLGLKNIPLTDDSINVLTSTFGTGLIMEDVKSLVNGKD
                     SFETEAFSNSNECQRLVKACNYMIAAQNRLLAINTCFTRKSFPFYSKFNLGRGFISNT
                     LALLSTIYSKEESYHFVSTASYKLDKTIRTVVSAQQDMNLEKILDTAVYISDKLQSLF
                     PTITREDIVLILQNVCLDSKPIWQSLEDKMKKINNSTASGFTVSNVILSHNSELNTIQ
                     KQIVWMWNMGLCSHRTLDFVIRYIRRRDVRYVKTEEQDESGNYVSGTMYKIGIMTRSC
                     YVELIASDQDVAVSLRTPFEILNEREYLFDTYRESIEKLLAEIMFDKVNIINQTTTDC
                     FLRTRRSCIRMTTDNKMIVKVNATSRQIRLENVKLVVKIKYENVNSDVWDIIESQKSL
                     VLRLPEVGEFFSDMYKTADSETETIKTIKNRLMTSLTFIEAFGNLSQQIKEIVDDDIR
                     ETMDEFLMNIRDTCLEGLENCKSVEEYDSYLDENGFNDTVELFENLLRTHDNFENEYS
                     PLFSEIVDKAKQYTRDLEGFKEILLMLKYSLINDASGFKSYRATGMHAVELMAKKHIE
                     IGEFNLLGMIQLIKACETCHNNDSILNLASLRNVLSRTYATFGRRIRLDHDLDLQNNL
                     MEKSYDFKTLVLPEIKLSELSREILKENGFVISGENLKMDRSDEEFVGLASFNVLRLD
                     EEEMYEGLIKEMKIKRKKKGFLFPANTLLLSELIKFLIGGIKGTSFDIETLLRNSFRP
                     DIFSTDRLGRLSSSVPALKVYATVYMEYKNVNCPLNEIADSLEGYLKLTKSRSKEHFL
                     SGRVKKALIQLRDEQSRTKKLEVYKDIANFLARHPLCLSEKTLYGRYTYSDINDYIMQ
                     TREIILSKISELDEVVETDEDNFLLSYLRGEEDAFDEDELDEEEDTD"
BASE COUNT     3362 a   1294 c   1692 g   2549 t
ORIGIN
        1 agagcaatca ggtaacaacg attttaagca aacatgaaca tccagaaaat acaaaaatta
       61 atagaaaatg gaaccacttt actgttgtct attgaggatt gtgtaggttc taaccacgat
      121 ctagctttgg atttacataa gagaaatagt gatgagatcc cagaagatgt gattataaat
      181 aataatgcaa aaaattatga gacaatgaga gagttaattg tcaaaatcac tgctgatggt
      241 gaaggactaa acaaagggat ggcaactgtg gatgtcaaaa agctaagtga gatggtctct
      301 ctgtttgagc aaaaatacct agaaacagag ttagcaaggc atgacatttt tggagagctg
      361 atctccaggc acctgagaat aaagcccaaa caaagaaatg aagtggagat agagcatgca
      421 ctaagagaat atctggatga actcaacaaa aagtcctgca ttaacaagct ctctgatgat
      481 gagtttgaga gaataaataa agaatatgta gcaactaatg ccacccctga taactatgtg
      541 atatataaag aatcaaaaaa cagtgagctt tgtttaatca tttatgattg gaaaatatct
      601 gtcgatgcca ggactgaaac caaacaatgg agaaatacct acaagaatat ttggaaatct
      661 ttcaaagata taaaagtgaa tggaaagcca ttcctggaag agcatcctgt tttcgtttct
      721 atagttatat tgaaacctat tgctgggatg ccaatcactg ttactagtag cagggttttg
      781 gagaaattcg aagattctcc atcagcattg cacggagaaa gaataaagca tgctaaaaat
      841 gccaaattgc taaatatttc ttatgttggg caaatagttg gaaccacacc cacagtggtg
      901 agaaactatt atgcaaacac tcaaagaatc aaatctgaag tcagaggaat cttaggtgat
      961 gattttggat ctaaagatgt gtttttcagt cactggacca gcaaatacaa agaaagaaat
     1021 cctactgaga tagcctattc cgaagatatt gaaagaataa ttgattcact tgttacagat
     1081 gaaatcccta gagaggaaat aatacatttt ttgtttggaa atttctgttt ccacattgaa
     1141 acaatgaatg accagcatat agctgacaaa tttaaagggt accaaaactc ttgtatcaat
     1201 ttaaaaatag agccaaaagc tgatttagct gatttgaaag accacttaat ccaaaagcag
     1261 caaatatggg aatctctgta tggaaaacac cttgagaaga tcatgcttag aattagagaa
     1321 aaaaagagaa aagaaaaaga gatacctgac ataaccacag cttttaacca gaatgctgct
     1381 gaatatgaag aaaggtatcc taactgtttc aatgatctct ctgaactaaa actaactttc
     1441 catgacttgg tccccagttt gaagatagaa ttgagctcag aggtagatta caacaacgca
     1501 attattaaca agtttcggga gagcttcaaa agttcttcaa gggttattta taatagccca
     1561 tatagtagca taaataacca aacaaataaa gcaagagata taacaaactt agttagactg
     1621 tgtttagcag agctaagttg tgatacaacg aaaatggaaa agcaggaact tgaagatgaa
     1681 atagatataa acaccgggag tatcaaagtt gagagaacaa aaaagtctaa agaatggaat
     1741 aagcaaggtt cgtgtttaac cagaaacaaa aatgaatttt gcatgaaaga tacaggcagg
     1801 gagaacaaaa ctacctattt taaaggctta gcagtaatga atataggaat gagttctaag
     1861 aaaagaattc taaaaaaaga agaaataaaa gaaaggatct ctaaaggctt ggaatatgat
     1921 acctctgaaa ggcaggctga cccaaatgat gattactcaa gtatagacat gtcttctctg
     1981 actcatatga aaaaactgat aaggcatgac aatgatgata gcttaagtgg taaaagattt
     2041 aagggctctt tttttctact tcataatttt aatataatag aggatggtaa gatcacatct
     2101 gttttcaata attatgctaa aaatcctgaa tgcttgtaca ttcaagattc agtactgaag
     2161 actgaattag agacttgcaa aaagataaac aaattatgca atgatctagc catttaccat
     2221 tactctgaag acatgatgca attctccaaa ggtttaatgg tggctgacag gtacatgact
     2281 aaagaaagtt tcaagatatt aaccacagca aatactagca tgatgctatt agcattcaaa
     2341 ggggatggaa tgaacaccgg aggatcggga gttccttaca tagcattgca tatagtggat
     2401 gaagacatgt cagatcattt taacatatgt tatactaaag aaatttatag ctatttccga
     2461 agtggtagta attacattta tataatgagg ccgcagagac taaaccaggt gaggctgctg
     2521 aggcttttca aaacgcctag taaagttcct gtatgttttc cacaattttc aaagaaagct
     2581 aatgaaatcg gaaaatcgct gaaaaataaa gatatagaaa aagtaaatct cttttctatg
     2641 acaatgactg taaaacagat attaataaat attgtgtttt catctgtcat gataggaact
     2701 gtgacaaagc tcagtagaat gggaattttt gatttcatgc ggtatgcagg ttttttgcga
     2761 ctatccgatt attctaacat aaaagaatac attagagaca aatctgatcc tgatataact
     2821 aactgtggca gatatctatt tcgtaatgga atcaaaaaac tattgttcag aatggaagat
     2881 ctcaatttaa gcacaaatgc caagcctgtt gttgtggacc acgaaaatga tattatagga
     2941 gggataacaa acttgaatat aaaatgtcct ataacaggat caactctact gacacttgag
     3001 gacctgtaca ataatgttta tttggctatt tatatgatgc ctaaatcact gcacaatcat
     3061 gttcacaatc taacaagctt attaaatctc cctgctgagt gggagctaaa gttcagaaaa
     3121 gaattaggtt tcaacatatt tgaagacata taccctaaga aagcaatgtt tgatgacaaa
     3181 gacctattct ccataaatgg agctttgaac gtgaaagcat tatctgatta ctatctagga
     3241 aatatagaaa atgttggttt aatgagatca gaaatagaaa ataaagaaga tttcctaagc
     3301 ccttgttata aaatatctac tttaaaatct tcaaaaaaat gctcacaatc aaacattata
     3361 agtactgatg agataataga gtgtcttcag gatgcaaaga ttcaagatat agaaaattgg
     3421 aaaggaaata acctagctat tataaaaggg cttataagaa cctacaatga ggagaaaaat
     3481 agattggtgg aattttttga agataattgt gtcaattcat tatatcttat agaaaagctt
     3541 aaagagataa ttagtagtgg atcaataact gtagggaaat ctgtaacatc taaattcata
     3601 agaaacaatc atcctttaac agtagaaaca tatctcaaaa caaaactata ttatagaaat
     3661 aatgtaacag ttttaaagtc taaaaaagtg tcagaggagc tctatgacct tgtaaaacag
     3721 ttccatgaca tgatggaaat agacctagat tctgttatga acctcgggaa aggtacagaa
     3781 ggaaaaaaac tcacattctt gcagatgctt gaatttgtca tgtccaaggc taaaaatgtc
     3841 accgggtctg tagattttct agtttctgtt tttgaaaaaa tgcagagaac caaaacagac
     3901 agagaaatat acttgatgag catgaaagtg aaaatgatgc tttattttat agagcataca
     3961 ttcaaacatg tagcgcagag tgatccatcg gaagccatat ctataagtgg agacaataaa
     4021 ataagagcac tttctacgtt atctttggac acaatcacgt cttacaatga tattttaaac
     4081 aaaaattcaa agaagtcaag attggctttc ctatctgcag atcagtcgaa atggtcggca
     4141 tcaggcctta ccacctataa atatgtttta gctatcatat taaatccaat tttaactact
     4201 ggtgaagcta gcttaatgat agaatgcatc ttaatgtatg ttaaattgaa gaaggtttgt
     4261 ataccaacag atattttttt gaatctaaga aaagctcaac aaacttttgg ggaaaatgaa
     4321 actgccatag gacttttgac caaaggcttg acgacaaaca cataccctgt tagcatgaat
     4381 tggttgcaag gcaatttaaa ttatctgtct tctgtttatc actcttgtgc aatgaaagct
     4441 tatcacaaca ctttggaatg ttacaaaaac tgtgatttcc aaactagatg gattgtgcac
     4501 tctgatgata atgcaacatc attaatagcc agtggagagg ttgataaaat gctgacagac
     4561 ttttcaagct catctctgcc agaaatgttg tttagaagca ttgaagctca tttcaaaagt
     4621 ttttgcataa ctttgaaccc aaaaaagagt tatgcttctt catcagaagt agagtttata
     4681 tctgaaagaa ttagtaaatg gagcgattat tcctctctat tgcaggcatt tagcaaactg
     4741 ttgcacagaa tcttcgcata taagttattt gatgatctaa tgtcactcag tatacatgtt
     4801 acaatgcttc tgagaaaagg ctgtcctaat gaagttatac cttttgctta tggggctgtg
     4861 caggtacaag cgttaagcat ctattcaatg cttcctggtg aagtgaatga tagtattaga
     4921 atttttaaca agcttggagt aagtttaaag tcaaacgaga ttcccacaaa catggggggc
     4981 tggttgacct ctcctataga gccgttgtct atattaggtc catcatcaaa tgatcaaatc
     5041 atctattaca atgtgataag agattttttg aacaaaaaaa gtttagaaga agtaaaagat
     5101 agtgtctctt cttccagtta tctacagatg agattcagag agctaaaaga aaagtatgaa
     5161 agaggaactc tggaagaaaa agataaaaag atgatatttc ttatcaatct gtttgagaaa
     5221 gcatcagtgt ctgaagattc agacgttcta acaattggga tgaaatttca aactatgtta
     5281 actcagatta taaaattacc taattttata aatgagaatg ctttaaacaa gatgtcaagt
     5341 tataaagatt tttcaaaact ttatcctaat ttaaaaaaga atgaagattt atataaaagc
     5401 actaagaact taaagataga cgaggatgct gttttagagg aagatgagtt atataagaag
     5461 attgcatcta gcttagaaat ggaatctgtc catgacataa tgataaaaaa tcctgaaaca
     5521 attctgatag caccattgaa tgatagagat tttttactta gtcagctgtt catgtacaca
     5581 agcccttcta aaagaaacca gttatcgaac caatctacag agaaacttgc tttagataga
     5641 gtgttaaggt caaaagctag aacatttgta aacatttctt ccactgtgaa gatgacttat
     5701 gaagaaaaca tggaaaagaa aatcttagaa atgctaaaat ttgatttaga ttcatattgt
     5761 tcatttaaaa catgtgtaaa tctagttatc aaggatgtta atttcagcat gctgattcca
     5821 atattagatt ctgcataccc ttgtgaatct aggaaaagag ataactacaa tttcaggtgg
     5881 tttcagactg agagatggat acctgttgtt gaaggctctc cgggactagt agtaatgcat
     5941 gctgtctatg gatcaaatta tatagagaac ttaggtttaa aaaacatccc tctaacagac
     6001 gatagtatta atgttttaac aagcacgttt ggaacaggtt taatcatgga agatgtaaaa
     6061 tccctagtta atggcaaaga cagcttcgaa acagaggctt ttagcaattc taatgaatgt
     6121 caaagattgg tgaaagcatg caattatatg atagcagcac aaaacaggct tttagcaatt
     6181 aacacatgct ttactaggaa aagctttccc ttctattcta agttcaatct agggagaggg
     6241 tttatctcaa acacattagc tctcctatcc accatctaca gtaaagaaga atcctatcat
     6301 tttgtttcta cagctagtta taaattagac aaaactatca gaactgtggt aagtgctcag
     6361 caagatatga acttagagaa aatactggac actgctgtat acatatcaga taaattgcag
     6421 tcacttttcc caacaattac aagagaggat atagttttaa tattgcaaaa tgtttgcctt
     6481 gacagtaaac ctatatggca gagtctagaa gacaaaatga aaaagattaa caattcaaca
     6541 gcaagtggct tcacagtgtc aaatgtgatt ctatcacata acagtgaatt gaacacaatc
     6601 cagaaacaaa ttgtctggat gtggaacatg ggtttgtgtt ctcacagaac attagatttt
     6661 gttatcaggt atattagaag aagggatgta agatatgtaa aaactgaaga acaagatgaa
     6721 tcaggaaatt atgtctctgg aactatgtac aaaataggga tcatgacaag aagctgctat
     6781 gtggaattga tagcatctga tcaagatgta gcagtttctt tgagaacacc atttgagata
     6841 ttgaatgaaa gagagtatct ttttgacaca tacagagaaa gtatagagaa attattggca
     6901 gaaattatgt ttgataaagt gaacataata aatcaaacaa ccacagattg ttttcttaga
     6961 accaggagat cttgcatcag aatgaccaca gacaacaaaa tgattgtaaa ggttaatgct
     7021 acatcaagac aaataagact agagaatgta aaattagttg taaagataaa atatgaaaat
     7081 gtgaattccg atgtatggga tattatagaa agccaaaaat ctctagtctt aaggctccct
     7141 gaagtagggg aatttttctc tgatatgtat aaaactgcag actctgaaac tgaaacaatc
     7201 aaaaccataa aaaacaggct tatgacttct ttaactttca tagaagcctt tggaaactta
     7261 tcacagcaga tcaaagagat tgtagatgat gatatcagag aaacgatgga tgaattctta
     7321 atgaacatcc gggatacctg cttagaaggt ttggaaaact gcaaaagtgt ggaagaatat
     7381 gatagctatc ttgatgaaaa tggatttaat gacacagtag aactattcga aaacttgcta
     7441 agaacacatg acaactttga aaatgagtat agtcctcttt tttcagagat tgttgacaaa
     7501 gcaaaacagt atactagaga tttagaaggt ttcaaagaaa tactgctcat gcttaaatat
     7561 tctctaataa atgatgcatc aggatttaaa agctatagag ccactggaat gcatgctgtt
     7621 gagctaatgg caaaaaagca catagagata ggggaattca acttgttagg aatgatccaa
     7681 ttgattaaag cttgtgaaac atgccacaac aatgactcta tattaaactt agcaagttta
     7741 aggaatgttc ttagcaggac atatgccaca tttgggagga gaataagatt ggatcatgat
     7801 ctggacttgc aaaacaactt aatggaaaaa agttatgatt tcaagacgct ggttttacca
     7861 gaaataaaat tatcagaact atctagggaa atactgaaag aaaatgggtt tgttatatct
     7921 ggagagaatc taaaaatgga taggtctgat gaagaatttg tgggtcttgc cagttttaat
     7981 gtgttgaggc tagatgagga agaaatgtat gaaggtttga tcaaagaaat gaaaattaaa
     8041 aggaaaaaga aagggttttt atttccagca aacacacttc tactaagtga gttgataaag
     8101 ttcttgattg gaggaataaa gggaaccagc tttgatatag agacattgtt acggaacagt
     8161 tttagaccag acatattttc aactgacaga ttgggaagat taagttccag tgtacctgca
     8221 ctcaaagttt atgcaactgt ttatatggaa tataagaatg tcaattgtcc tttaaatgag
     8281 atagctgaca gcttagaagg ttatctaaaa ctgacaaaaa gcaggtccaa ggaacatttc
     8341 ttgtctggaa gagttaaaaa agctttgata caattaagag atgaacaatc gcgaactaaa
     8401 aaactagagg tctataagga tatcgcaaat ttccttgcta ggcacccact atgtttatca
     8461 gaaaaaacat tgtatggaag atatacctac tctgatatca atgattatat catgcaaaca
     8521 agagagatta ttttgagtaa aataagtgag ttggacgagg ttgttgaaac agatgaagac
     8581 aatttcttgc ttagttatct aagaggggaa gaagatgcct ttgatgaaga tgagcttgat
     8641 gaagaagaag acacagatta aattgaaagt aatgactaac aatccatgaa taacagatta
     8701 gatataactt agaatataaa tttattgcta ttttagaatt agattagatc tacttagcct
     8761 aaaacaattt ggtgaaccaa atctatagtg tatataaatg tagagtcccg gtatagtttc
     8821 actggaggga attcttatgt aatttgtaaa gtctggctgt ggagaggtta tatgttttag
     8881 ttgtacctga ttgctct
//