From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Plasmid HG1 (from Alcaligenes eutrophus) carbon dioxide fixation
Date: 21 Dec 92 20:34:30 GMT
Distribution: bionet
Checksum: 59877 6

LOCUS       PG1CFXXYEP   2655 bp ds-DNA             BCT       19-NOV-1992
DEFINITION  Plasmid HG1 (from Alcaligenes eutrophus) carbon dioxide fixation
            operon D-ribulose-5-phosphate 3-epimerase (cfxEp), cfxXp and cfxYp
            genes, complete cds.
ACCESSION   M64172
KEYWORDS    D-ribulose-5-phosphate 3-epimerase; carbon dioxide fixation;
            cfx operon; cfxE gene; cfxX gene; cfxY gene.
SOURCE      Plasmid pHG1 DNA.
  ORGANISM  Plasmid pHG1
            Prokaryotae.
REFERENCE   1  (sites)
  AUTHORS   Kusian,B., Yoo,J.-G., Bednarski,R. and Bowien,B.
  TITLE     The Calvin cycle enzyme pentose-5-phosphate 3-epimerase is encoded
            within the cfx operons of the chemoautotroph Alcaligenes eutrophus
  JOURNAL   J. Bacteriol. 174, 7337-7344 (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     CDS             157..1110
                     /note="cfxX"
                     /gene="cfxX"
                     /codon_start=1
                     /translation="MSAPETTAPLQPPAAQAASLPGSLAESLASSGITELLAQLDREL
                     IGLKPVKARIRDIAALLLVDKLRAARGFSAGAPSLHMCFTGNPGTGKTTVAMRMAQIL
                     HQLGYVRRGHLVAVTRDDLVGQYIGHTAPKTKEILKKALGGVLFIDEAYYLYRPENER
                     DYGQEAIEILLQVMENNRDDLVVILAGYKDRMDRFFESNPGMSSRVAHHVDFPDYQLD
                     ELRQIADLMLAEMQYRFDDESRAVFADYLARRMAQPHFANARSVRNALDRARLRHASR
                     LLDDAGTVADDRTLTTITASDLLASRVFSKAAPAAQTPAKE"
     CDS             1114..1878
                     /note="cfxY"
                     /gene="cfxY"
                     /codon_start=1
                     /translation="MQALIFDVDGTLADTETAHLQAFNAAFAEVGLDWHWDAPLYTRL
                     LKVAGGKERLMHYWRMVDPEEARGCKVKETIDAVHAIKTRHYAERVGAGGLPLRPGIA
                     RLIAEAGEAGLPLAIATTTTPANLDALLQAHLGADWRGRFAAICDAGTTAIKKPAPDV
                     YLAVLERLGLEAGDCLAIEDSGNGLRAARAAGIPTVVTPTTFSAQDSFEGALLVLPHL
                     GDPAEPMPQHVPGAAHRWADLAALRAWHHGTLIEAT"
     CDS             1880..2605
                     /gene="cfxE"
                     /product="D-ribulose-5-phosphate 3 epimerase"
                     /codon_start=1
                     /translation="MHATEPNTGHGSQRAIRLAPSILSADFARLGEEVCAIEAGGADL
                     VHFDVMDNHYVSNLTIGPLVCEAIRPLVSIPIDVHLMVEPVDALIPMFAKAGANLISF
                     HPEASRHVDRTIGLIRDHGCKAGLVLNPATPLSWLDHTLDKLDLVLLMSVNPGFGGQA
                     FIPGVLDKVRQARARIDRQVAAGGRPVWLEIDGGVKADNITEIARAGADTFVAGSAVF
                     GAPDADGGYRGILHRLREAATIT"
BASE COUNT      435 a    957 c    858 g    405 t
ORIGIN      
        1 gcggcaccgg ctgaaccgcc gcgcggcgct gccacgacgc tgccgccggc gatttcctga
       61 ctgtgccatc ccacggttgc gccgccgcca ggctgcgcgt ccggggggag ctgcgcctcg
      121 aatttatcga ggtgaacgat cctcacggag cctgccatgt ccgcacccga aacgaccgca
      181 ccgctgcagc caccagctgc ccaggccgca tcgctgcctg gatcgctggc cgaatcgctg
      241 gccagctcgg gcatcaccga gctgctggcc cagctcgacc gtgaactgat cggactgaag
      301 ccggtgaaag cgcgcattcg cgatattgcc gccttgctgc tggtggacaa gctgcgcgcc
      361 gcgcgcggct tcagcgccgg tgcgcccagc ctgcatatgt gcttcaccgg taatcccggc
      421 actggcaaga ccaccgtggc tatgcgcatg gcgcagatcc tgcaccagct tggctacgtg
      481 cggcgcggcc acctggtggc ggtgacccgc gacgacctgg tcggccagta catcggccat
      541 accgcgccca agaccaagga gatcctgaag aaggccttgg gcggggtgct cttcatcgac
      601 gaggcctact acctctaccg cccggagaac gagcgcgact acggccagga ggccatcgag
      661 atcctgctgc aggtgatgga gaacaaccgc gatgacctgg tggtgatcct ggccggctac
      721 aaggaccgca tggaccgctt cttcgagtcc aacccgggca tgtcctcgcg cgttgcccac
      781 catgtcgatt tccctgacta ccagctcgac gagctgcgtc agatcgccga cctgatgctg
      841 gccgagatgc agtaccgctt tgacgacgaa agccgggccg tgtttgcgga ttacctggcc
      901 cggcgcatgg cgcagccgca ctttgccaat gcccgcagcg tgcgcaatgc gctggaccgc
      961 gcgcggctgc gccatgcctc gcgcctgctg gacgatgccg gcacggtcgc cgacgaccgt
     1021 accctgacca ccatcacggc gtctgacctg ctggccagcc gcgtgttttc gaaggccgcg
     1081 ccagccgcac agacgccggc caaggagtaa gccatgcaag ccctgatctt cgatgtcgac
     1141 ggcacgctcg ccgataccga aaccgctcac ctgcaagcct tcaatgccgc cttcgccgag
     1201 gtcggcctgg actggcactg ggacgcgccg ctctacacgc gcctgctcaa ggtcgccggc
     1261 ggcaaggagc gcctgatgca ttactggcgc atggtcgacc cggaagaggc ccgcggctgc
     1321 aaggtgaagg aaaccatcga cgccgtgcac gccatcaaga cccgccacta cgccgagcgc
     1381 gttggggcgg gcggcctgcc gctgcgcccg ggcattgccc gcctgatcgc agaggccggc
     1441 gaggccggcc tcccgctcgc gattgccacc accaccacgc cggccaacct tgacgcgctg
     1501 ctgcaggcgc accttggcgc ggactggcgc gggcgctttg ctgccatctg cgacgccggc
     1561 accaccgcga tcaagaagcc tgcgcccgat gtttacctgg cggtgctgga gcggctcggc
     1621 ctggaggccg gcgattgcct ggccatcgag gactcgggga acggattgcg cgccgcccgg
     1681 gcggccggca ttcccaccgt tgtcacgccc accaccttca gcgcgcagga ttccttcgag
     1741 ggcgcgctgc tggtgctgcc gcatcttggc gatcccgccg aacccatgcc ccagcacgtg
     1801 cccggggcgg cacaccgctg ggccgacctt gccgcgttgc gcgcctggca ccacggcacc
     1861 ctgatcgagg cgacctgaca tgcatgccac cgaacccaac accggccatg gcagccagcg
     1921 cgccatccgc ctggcgccat ccatcctgtc ggccgatttc gcgcgcctgg gcgaagaggt
     1981 gtgcgcgatc gaggccggcg gcgcggacct ggtgcacttc gatgtgatgg acaaccacta
     2041 tgtgtccaac ctgaccatcg gcccgctggt gtgcgaggca atccggccgc tggtttcgat
     2101 ccccatcgac gtgcacctga tggtggagcc ggtcgatgcg ctgatcccga tgttcgccaa
     2161 ggccggcgcc aacctcatca gcttccatcc ggaagcgagc cgccatgtgg accgcaccat
     2221 cggcctgatc cgcgaccacg gctgcaaggc cggcctggtg ctgaacccgg ccacgccgct
     2281 gagctggctg gaccacacgc tggacaagct cgacctggtg ctgctgatga gcgtgaaccc
     2341 gggcttcggc ggccaggcct tcattccggg cgtgctggac aaggtgcgcc aggcacgggc
     2401 gcgcatcgac cggcaagtgg ccgccggcgg gcggccggtc tggctggaga tcgacggcgg
     2461 cgtcaaggcc gacaacatca ctgagatcgc ccgtgcgggc gccgacacct tcgtggccgg
     2521 cagcgccgtg ttcggcgcgc cggatgccga cggcggctac cggggcatcc tgcaccgctt
     2581 gcgcgaggcc gccaccatca cgtagcccgc acggcaccac acaagaatac atagccaatc
     2641 tataggagac ctgtc
//