From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: P.sativum pcr gene for protochlorophyllide reductase
Date: 21 Jul 92 10:07:46 GMT
Distribution: bionet
Checksum: 23845     7

LOCUS       PEAPCR       2844 bp ds-DNA             PLN       21-JUL-1992
DEFINITION  P.sativum pcr gene for protochlorophyllide reductase
ACCESSION   X63060
KEYWORDS    pcr gene; protochlorophyllide oxidoreductase.
SOURCE      Pisum sativum DNA.
  ORGANISM  Pisum sativum
            Eukaryota; Plantae; Embryobionta; Magnoliophyta; Magnoliopsida;
            Rosidae; Fabales; Fabaceae.
REFERENCE   1  (bases 1 to 2844)
  AUTHORS   Spano,A.J., He,Z., Michel,H., Hunt,D.F. and Timko,M.P.
  TITLE     Molecular cloning, nuclear gene structure, and developmental
            expression of NADPH protochlorophyllide oxidoreductase in Pea
            (pisum sativum L.)
  JOURNAL   Plant Mol. Biol. 18, 967-972 (1992)
  STANDARD  full automatic
COMMENT     *source: cell_line=variety: Progress No.9; *source: clone_library=
            EMBL4;
            From EMBL    entry PSPCR;  dated 10-JUN-1992.
FEATURES             Location/Qualifiers
     mRNA            join(313..406,498..599,898..1426,1544..1902,2131..2286)
                     /gene="pcr"
     CDS             join(353..406,498..599,898..1426,1544..1902,2131..2286)
                     /EC_number="1.3.1.33"
                     /product="protochlorophyllide reductase"
                     /gene="pcr"
                     /codon_start=1
                     /translation="MALQTASMLPASFSIPKEGKIGASLKDSTLFGVSSLSDSLKGDF
                     TSSALRCKELRQKVGAVRAETAAPATPAVNKSSSEGKKTLRKGNVVITGASSGLGLAT
                     AKALAESGKWHVIMACRDYLKAARAAKSAGLAKENYTIMHLDLASLDSVRQFVDNFRR
                     SEMPLDVLINNAAVYFPTAKEPSFTADGFEISVGTNHLGHFLLSRLLLEDLKKSDYPS
                     KRLIIVGSITGNTNTLAGNVPPKANLGDLRGLAGGLTGLNSSAMIDGGDFDGAKAYKD
                     SKVCNMLTMQEFHRRYHEETGITFASLYPGCIATTGLFREHIPLFRTLFPPFQKYITK
                     GYVSEEESGKRLAQVVSDPSLTKSGVYWSWNNASASFENQLSQEASDAEKARKVWEVS
                     EKLVGLA"
     TATA_signal     279..286
     prim_transcript 313..>2286
                     /gene="pcr"
     exon            313..406
                     /number=1
     intron          407..497
                     /number=1
     exon            498..599
                     /number=2
     intron          600..897
                     /number=2
     exon            898..1426
                     /number=3
     intron          1427..1543
                     /number=3
     exon            1544..1902
                     /number=4
     intron          1903..2130
                     /number=4
     exon            2131..>2286
                     /number=5
     polyA_signal    2449..2454
BASE COUNT      830 a    511 c    570 g    933 t
ORIGIN
        1 gaattcactt tctatccaaa aaattatcta caagcctcaa tcgttttctt cattgatgat
       61 attattataa tattattatc acagaacaca taggccataa ctagtagtaa taatgaagtc
      121 tgtgacacta ttgtgacttc tattggcaca tatattaaaa tatctttttc ttttatgaaa
      181 tccaaacctc aaaaccttat tggttggttt gaaaggatgt ggcaaatgca catactgcta
      241 attatcttat cctcacattt catgaataac tccttcactt tataaactca atttcaaatc
      301 catttttata ataacaccaa cactttttat tacataaaat tttcatatag taatggctct
      361 tcaaactgct tctatgcttc ctgcttcttt ctccattccc aaagaggttt gttgttcttc
      421 ttgttgttct tgtatatata ctttttcaaa gttgataatt gaactgaatt tgtggaggat
      481 tatttttgtg tttttagggg aagattggtg cgtctctcaa ggactcgact ctttttggtg
      541 tttcatcatt gtctgattct ttgaaaggtg atttcacctc ttctgcattg aggtgtaagg
      601 taactcaaag cataactttg gtttttatat ttctcaatta gtcattcaat tgttaatact
      661 tatacttgag tttgagtata taaaatatgt cacatgtata aataaatgtt aggaaaattt
      721 ctaagtgctt gtttggagtc acggtgtatt tgtcagagtc acggtgtggt catgacaaat
      781 tcaccgtagt tccaaacatg ccataaaggg ggcgtgttga ttataagaaa tagtatattg
      841 ccggtttatg ttttcgggac attgtctaat ttatgttttc ttgctatata taagagggaa
      901 ttacgacaaa aagttggtgc tgtgagggcg gagacagcgg ctccggccac tccagcagtt
      961 aacaagtcat catctgaagg gaagaagacg ttgaggaaag gaaatgttgt gatcactggt
     1021 gcttcatctg gtttaggtct agctactgct aaggctttgg ctgaatcagg aaaatggcat
     1081 gtaattatgg cttgcaggga ctacctcaaa gctgcaagag ctgcaaaatc ggctggattg
     1141 gctaaggaaa actacacgat tatgcattta gaccttgcgt ctcttgacag tgttcgtcaa
     1201 tttgttgata acttcagaag atctgaaatg ccattggatg tgcttattaa caatgctgct
     1261 gtttactttc caactgctaa ggaacctagt ttcacggcgg atggctttga aattagtgtt
     1321 gggacaaacc atcttgggca ttttcttctt tcgcgccttt tgcttgagga ccttaaaaaa
     1381 tccgattacc cttcaaagcg cttgatcatt gtcggctcaa tcacaggtag agttcattcg
     1441 tcgaggcttt tgatgtatct gcgaccacaa atgactgcga tctctgcaat aactaggatt
     1501 attattttgt tgtgttttgt actgattgtg tataattaaa caggtaacac aaacacattg
     1561 gctggaaatg ttccgccgaa ggctaacctc ggtgacttga gaggacttgc tggtggcttg
     1621 actggactta acagttcggc catgatagac ggtggagatt ttgatggtgc caaggcatac
     1681 aaggacagca aagtctgtaa tatgctcaca atgcaagagt tccatagaag atatcatgag
     1741 gaaactggaa tcacatttgc ttccctttac cctggttgca ttgccacaac aggcttgttt
     1801 agagagcaca ttcccttgtt cagaactcta ttccctccat tccaaaagta cataaccaaa
     1861 ggctacgtct cagaagaaga atcgggaaag agacttgctc aggtaattag agacttttga
     1921 ttaacttatt tgaacttatc taggagcata aataaccatt cggaagactc tttaaaagag
     1981 cttatgaaaa caacttataa acatttatag tcgctcagtt ccaaacatgg tctaatagca
     2041 gtaacactcg aggaaatgtg cttgtgagaa gtgtgtgaat gattgaagta ttgtcaattc
     2101 tcatgcatgg ttgtgttgtt ttcaatgtag gttgtaagtg atccaagcct aacaaaatct
     2161 ggtgtttact ggagctggaa taacgcttct gcttcatttg agaaccagct atcacaagag
     2221 gccagtgatg ccgagaaggc tcgcaaggtg tgggaggtta gtgagaagct tgttggtttg
     2281 gcctaaagtg agagccccca acatctatgc cgagttagag tctgaggcat tgccatggaa
     2341 ggaagatgat gccccagaag agtgaaatga tgatatcatg ttgagtgcat taattgttgt
     2401 tgtgttagat aggaactttt tttgtcttta tgtaggagtt gatgaatcaa taaaatcatt
     2461 caaccatttg gcaaagtgct tataacttct ctgaaataac tgtccatagt gaaatttctc
     2521 ttaaaatatt gtttaactcc agcactacac tttcgagatt ctaacaaaaa ctaccttcta
     2581 gattctaaac aattcaagat ccaaagcaca aatcaaatac atctcatcta ccgaaatatt
     2641 atggttttgg tacgggaatt tacatctgta atatacatat gttgctttat ctatcatgaa
     2701 ctcaaagttc atagccagga aaccttgcca cttgtggatc aatcatgtct agaaaggtat
     2761 atgccattcc tccaagacct tcgaataagg aataaggtcg atccccaccg tgcatttttc
     2821 cttctgtgat caatttttga gctc
//