From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Escherichia coli periplasmic putrescine binding protein (potF)
Date: 3 Sep 92 10:00:40 GMT
Distribution: bionet
Checksum: 54616    12

LOCUS       ECOPOTFGHI   5877 bp ds-DNA   Circular  BCT       03-SEP-1992
DEFINITION  Escherichia coli periplasmic putrescine binding protein (potF)
            gene, complete cds, (potG) gene, transmembrane protein (potH) and
            (potI) genes, complete cds.
ACCESSION   M93239
KEYWORDS    periplasmic putrescine binding protein; potF gene; potG gene;
            potH gene; potI gene; transmembrane protein.
SOURCE      Escherichia coli (strain DR112) DNA.
  ORGANISM  Escherichia coli
            Prokaryotae; Gram-negative facultatively anaerobic rods;
            Enterobacteriaceae.
REFERENCE   1  (bases 1 to 5877)
  AUTHORS   Pistocchi,R., Kashiwagi,K., Miyamoto,S., Nukui,E., Sadakata,Y.,
            Kobayashi,H. and Igarashi,K.
  TITLE     Characteristics of the gene for a putrescine transport system that
            maps at 19 min on the Escherichia coli chromosome
  JOURNAL   Unpublished (1992)
  STANDARD  full staff_entry
FEATURES             Location/Qualifiers
     -10_signal      1099..1104
                     /note="putative"
     sig_peptide     1260..1337
                     /gene="potF"
                     /codon_start=1
     CDS             1260..2372
                     /product="periplasmic putrescine binding protein"
                     /gene="potF"
                     /codon_start=1
                     /translation="MTALNKKWLSGLVAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLNVR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYANANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     mat_peptide     1338..2369
                     /product="periplasmic putrescine binding protein"
                     /gene="potF"
                     /codon_start=1
     CDS             2386..3600
                     /note="consensus nucleotide binding sequences"
                     /gene="potG"
                     /codon_start=1
                     /translation="MPEGRTTPAGNSHHYGALAHIQCRRAAVNDAIPRPQAKTRKALT
                     PLLEIRNLTKSYDGQHAVDDVSLTIYKGEIFALLGASGCGKSTLLRMLAGFEQPSAGQ
                     IMLDGVDLSQVPPYLRPINMMFQSYALFPHMTVEQNIAFGLKQDKLPKAEIASRVNEM
                     LGLVHMQEFAKRKPHQLSGGQRQRVALARSLAKRPKLLLLDEPMGALDKKLRDRMQLE
                     VVDILERVGVTCVMVTHDQEEAMTMAGRIAIMNRGKFVQIGEPEEIYEHPTTRYSAEF
                     IGSVNVFEGVLKERQEDGLVLDSPGLVHPLKVDADASVVDNVPVHVALRPEKIMLCEE
                     PPANGCNFAVGEVIHIAYLGDLSVYHVRLKSGQMISAQLQNAHRHRKGLPTWGDEVRL
                     CWEVDSCVVLTV"
     CDS             3610..4563
                     /product="transmembrane protein"
                     /gene="potH"
                     /codon_start=1
                     /translation="MSTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     CDS             4560..5405
                     /product="transmembrane protein"
                     /gene="potI"
                     /codon_start=1
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGLSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARRG"
BASE COUNT     1300 a   1403 c   1640 g   1534 t
ORIGIN
        1 gatatccgct gtctggttgt tggcgatgaa gtggtcgctg cgattgaacg gcgggcgaaa
       61 gagggcgatt ttcgttccaa tttgcatcgt ggcggcgcgg caagcgtcgc cagtatcaca
      121 ccacaggagc gtgaaatcgc gataaaagcc gcgcgaacga tggcgctgga cgttgctggt
      181 gtggatattc tgcgtgctaa tcgcgggccg ttggtgatgg aggtgaatgc gtcgccgggg
      241 ctggaaggaa tagaaaaaac caccggtatc gacatcgcgg gtaaaatgat ccgctggatc
      301 gaacgccacg ctacgacaga atattgcctg aaaacgggtg gttagtcgca atcacattac
      361 tgatcatggt tttgcctgcg ctttttgcgt aagctgtgcc ggtcttttta tcgaaagagg
      421 ttgtacaaaa ttatgacatc gctggtcgtt cctggtctgg atacgctgcg tcaatggctc
      481 gatgacctgg ggatgagttt ttttgaatgt gataactgtc aggctctgca tctgccccat
      541 atgcagaatt tcgacggtgt ctttgatgcc aaaatcgatc tgatcgataa ctcgatcctg
      601 ttttctgcca tggcggaagt ccgaccttca gccgtattgc cgctggcggc ggatttatct
      661 gccatcaatg ccagttcgct gaccgtgaaa gcatttcttg atatgcagga tgataatctg
      721 ccaaagctgg tggtttgcca gtctttatcc gttatgcagg gcgtaaccta tgagcagttt
      781 gcatggttcg tgcgtcagag cgaagagcag atttcgatgg tcattcttga agctaatgcc
      841 catcaactgc tgttaccgac tgatgatgaa gggcaaaaca acgttaccga aaactatttc
      901 ctccactgat aactcctttc gagcacgcag tcgctggtgc agtggctgcg cgctgcaaaa
      961 ttatctgctg tttttaacct tttcttaaag attatttcac ttctcttgtg tcgatttggc
     1021 tttatcacat agagcaaata tgcataaaaa tttgttaaat accgtttttt aatccgagct
     1081 atagtctcaa accctggcta aagttattct tgcgatgctt ttatatagcg agcagtgctg
     1141 gccgggagaa agttctcttt tcttacaccg cgccgataaa aaatatgcac gtttattgca
     1201 tatctttcag tgtgacaact tttgttcgtt tgttaacgaa ctttcagaag gaaagagata
     1261 tgaccgcctt aaataaaaaa tggctatcgg gtctggttgc gggtgctctg atggccgtct
     1321 ctgtcggcac gctcgcggct gaacaaaaaa cactccacat ttataactgg tctgattata
     1381 tcgccccgga cacggtggcc aattttgaaa aagaaaccgg tattaaagtc gtctacgatg
     1441 ttttcgactc taacgaagta ctggaaggca aattaatggc cgggagtacc ggctttgatc
     1501 tggtggttcc atctgccagc tttctggagc gccagttgac tgcgggagtt ttccagccgc
     1561 tggacaaaag caaattgccg gagtggaaga atctcgaccc ggaactgctg aagctggtcg
     1621 ccaaacacga tcccgacaat aaatttgcta tgccctatat gtgggcgacg accgggattg
     1681 gctataacgt tgataaagtt aaagcggtgc tgggcgaaaa cgcgcccgtc gatagctggg
     1741 acttgatcct caaacctgaa aatctggaaa aactgaaaag ctgcggtgtc tctttcctgg
     1801 atgcgccaga agaagttttt gctaccgtgt tgaattatct cggcaaagat cccaacagca
     1861 ctaaagcgga tgattacacc ggaccggcaa cagatctgct gttaaacgtg cgcccgaaca
     1921 ttcgttattt ccattcatct caatacatta acgacctggc aaacggcgat atttgcgtcg
     1981 ctatcggctg ggcaggtgat gtctggcagg cgtcaaaccg cgcgaaggaa gcgaagaatg
     2041 gcgtgaatgt ctcgttctcg attccaaaag aaggggcgat ggcgttcttt gatgtattcg
     2101 ccatgcctgc ggatgccaaa aacaaagacg aagcctatca gttcctgaat tacctgctgc
     2161 gcccggatgt agtagcgcat atttccgacc atgtgttcta tgccaacgcc aataaagcag
     2221 ccacgccgct ggtgagtgcg gaagtccgtg agaacccagg tatttatccg cctgcggatg
     2281 ttcgtgcgaa gctgttcact ctgaaagtgc aggatccgaa aatcgaccgt gtgcgcaccc
     2341 gcgcgtggac caaagtgaag agcggaaaat aatccgcagt cgtagatgcc ggaggggcgc
     2401 accacacccg ccggcaattc gcaccattat ggtgcgcttg cacacattca atgccggaga
     2461 gcagccgtga atgacgctat ccctcgcccg caggcgaaaa cccgtaaggc gctgacgccg
     2521 ctattagaaa tccgcaacct gaccaaatcc tacgatggtc aacatgcggt ggatgatgtc
     2581 agcctgacca tctacaaagg tgaaatcttc gcgctgctgg gcgcatccgg ctgtggcaag
     2641 tccacgctgc tgcgtatgct ggcaggtttc gaacaacctt ctgccggaca gataatgctt
     2701 gatggcgtcg atttgtcaca ggttccgcct tacctgcgcc ccatcaatat gatgtttcag
     2761 tcttacgcgc tgtttccgca tatgaccgtg gaacagaaca tcgcttttgg cctgaaacag
     2821 gacaaactac cgaaagcgga aattgccagc cgggtcaatg agatgctcgg cctggtgcat
     2881 atgcaggagt tcgccaaacg caaaccgcat cagctttccg gtggtcagcg acaacgtgtg
     2941 gccctggccc gaagccttgc gaagcgcccg aaactattac tgctcgatga gccgatgggc
     3001 gcgctggata aaaagctgcg tgacaggatg cagcttgaag tggtggatat tctggagcgc
     3061 gtcggtgtga cttgtgtgat ggtcacccac gatcaggaag aggcgatgac catggcgggg
     3121 cgcatcgcca ttatgaatcg tgggaaattt gtccagattg gcgaaccgga agagatctac
     3181 gagcatccga ctacccgcta tagcgccgaa tttattggct cggtaaacgt ctttgaaggc
     3241 gtactcaaag agcgtcagga agatggcctg gtgcttgatt cgccggggct ggtgcatcca
     3301 ctgaaagtcg acgcggatgc ctcggtggtc gataacgtgc cggtacatgt ggcgctgcgc
     3361 ccggaaaaaa tcatgctttg cgaagagccg cccgccaatg gttgtaactt cgcggtgggg
     3421 gaggtgatac acattgccta tctcggcgat ctttcggtgt atcacgttcg tctgaaaagt
     3481 gggcagatga ttagcgccca gctacaaaac gcccatcgtc atcgtaaagg gttaccgacc
     3541 tggggcgacg aagtgcgttt gtgctgggaa gtggacagct gtgtggtgct gacggtttaa
     3601 ggagcaaaga tgagtacact tgaacctgct gcccagtcga aaccgccggg cggatttaag
     3661 ctgtggttgt cgcagctgca aatgaagcat gggcgcaaac tggtcattgc gttgccatat
     3721 atctggttga tcttgctgtt tctgctgcca tttctgattg tctttaaaat aagcctggcg
     3781 gagatggcgc gcgctattcc accttatacc gaactgatgg agtgggctga cgggcaactg
     3841 tccatcactc ttaatctcgg taattttctg caactgaccg acgatccgct ctatttcgat
     3901 gcttatctcc agtcgctaca ggtggctgcg atttcgacat tttgctgttt actgatcggc
     3961 tatccgctgg cgtgggcggt ggcgcacagt aagccttcga cccgtaatat tttattacta
     4021 ctggtgatcc tgccgtcgtg gacctcgttt ctgatccgcg tttatgcctg gatgggaata
     4081 ttaaaaaaca acggtgtgct gaataatttt ctgctgtggc tgggggttat cgatcaaccg
     4141 ctgaccattc tgcataccaa tctggccgtt tatatcggca ttgtttacgc ttacgtgccg
     4201 tttatggtac tgccgattta taccgcgttg attcgtattg attattcgct ggtggaagca
     4261 gcgctggatc tcggtgcacg accgctgaaa acgttcttta ctgtgatcgt gccgctgact
     4321 aaaggtggga ttattgccgg atcgatgctg gtgtttatcc cggctgtggg cgagtttgtg
     4381 atcccggaac tgctcggtgg cccggacagc atcatgatcg ggcgcgtgct atggcaagag
     4441 tttttcaata accgcgactg gccggtggcc tcggcggtag cgatcatcat gttgctgctg
     4501 ctaattgtgc cgataatgtg gttccacaaa caccagcaaa aaagcgtggg agaacacgga
     4561 tgaataattt accggtagtt cgttcgccct ggcggattgt gattttgctg ctgggcttta
     4621 cctttctcta cgcgccaatg ctgatgctgg ttatctattc gtttaacagc tcgaagctgg
     4681 tgacggtgtg ggccggctgg tcaacgcgct ggtatggtga gttattgcgc gatgacgcga
     4741 tgatgagtgc ggttggttta agtctgacaa ttgcggcctg tgcggcgacg gcggcggcga
     4801 tcctcggaac tattgcggcg gtggtgctgg tgcgctttgg caggtttcgc ggatcaaatg
     4861 gctttgcctt tatgatcacc gcgccgctgg taatgccaga tgtcatcacg ggcttgtcgc
     4921 tgttgttatt attcgtcgcg cttgctcatg ccattggctg gcctgcggac cgcggtatgc
     4981 tcaccatctg gctggcgcat gtcacttttt gtacggctta tgtggcggtc gttatttctt
     5041 caagattgcg ggaactggat cgctcgatag aagaagcagc gatggatctc ggtgcgacgc
     5101 cgctgaaagt gtttttcgtc attacgctac cgatgatcat gcccgcgatc atttctggct
     5161 ggttactggc ttttactttg tcgcttgatg atctggtgat cgccagcttt gtttccgggc
     5221 cgggagccac cacgttaccg atgctggtct tttccagcgt gcggatgggg gtgaatccgg
     5281 aaatcaacgc cctggcaaca ttgatcctcg gcgcggtcgg aattgtcgga tttatcgcct
     5341 ggtatctgat ggctcgcgca gaaaaacagc ggatacgcga catccagcgt gcaagacgtg
     5401 gctgaagaca ctaaaatttg ccaacctggc tacataatgc cgcgcatgtc gcggcattgt
     5461 tttcatggaa gacgaaacgt tgggattttt taagaaaaca tcttcatctc atgctcgcct
     5521 gaatgtgcct gcgctggtgc aggtggcggc gctcgcatta ttatgatccg tggcctcgac
     5581 gtgctgatga ttttcaatac gctgggcgtg cgcggtattg gcgagttcat tcatcgcagc
     5641 gtacaaacct ggagtttaac gctggtcttt ttaagcagtc tggtgctggt tttcattgag
     5701 atctggtgtg cgttttcact ggtgaaaggg cgtcgctggg cgcgctggct atatctgctg
     5761 acacaaatca ccgccgcaag ttacttgtgg gcggcttcgc tggggtacgg ttatccggag
     5821 ctgttcagca ttcccggtga atcaaaacgt gaaatcttcc atagcctgat gctgcag
//