From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: pepN=zinc-metallo aminopeptidase [Lactococcus lactis, Genomic, 3200
Date: 6 Jan 93 07:03:01 GMT
Distribution: bionet
Checksum: 20076 7

LOCUS       S108327      3200 bp    DNA             BCT       30-NOV-1992
DEFINITION  pepN=zinc-metallo aminopeptidase [Lactococcus lactis, Genomic, 3200
            nt]
ACCESSION   S39955
KEYWORDS    .
SOURCE      Lactococcus lactis
  ORGANISM  Lactococcus lactis
            Unclassified.
REFERENCE   1  (bases 1 to 3200)
  AUTHORS   Tan,P.S., van Alen-Boerrigter,I.J., Poolman,B., Siezen,R.J., de
            Vos,W.M. and Konings,W.N.
  TITLE     Characterization of the Lactococcus lactis pepN gene encoding an
            aminopeptidase homologous to mammalian aminopeptidase N.
  JOURNAL   FEBS Lett. 306, 9-16 (1992)
  STANDARD  full automatic
COMMENT     This entry [NCBI gibbsq 108327] was created by the journal scanning
            component of NCBI/GenBank at the National Library of Medicine.
            This sequence comes from Fig. 3.
FEATURES             Location/Qualifiers
     CDS             324..2864
                     /note="Description: zinc-metallo aminopeptidase;  For the
                     protein sequence (NCBI gibbsq 108329):  Method: conceptual
                     translation with partial peptide sequencing.  This
                     sequence comes from Fig. 3."
                     /gene="pepN"
                     /codon_start=1
                     /translation="MAVKRLIETFVPENYKIFLDIDRKTKKIKGQVAITGEAKDTVVS
                     FHTKGLHFNKVRAFSVDTNFIENEEDEEIVVKIGETGRVTVSFEYEAELTDNMMGIYP
                     SYYEVNGEKKMLIGTQFESHFARQAFPSIDEPEAKATFDLSVKFDEEEGDIIVSNMPE
                     LLNINGIHVFERTVKMSSYLLAFVFGELQYKKGKTKSGVEVGAFATKAHSQAALDFPL
                     DIAIRSIEFYEDYYQTPYPLPHSWHIALPDFSAGAMENWGCITYREVCMLVDPENATI
                     QSKQYVATVIAHELAHQWFGDLVTMQWWDDLWLNESFANNMEYVCMDALEPSWNVWES
                     FSISEANMALNRDATDGVQSVHVEVTHPDEIGTLFDPAIVYAKGSRLMVMLRKWLGDE
                     DFAAGLALYFKRHQYGNTVGDNLWDALAEVSGKDVAAFMHSWVNQPGYPVVTAEVVDD
                     TLILSQKQFFVGEGVDKGRLWNVPLNTNWTGLPDLLSSEKVEIPGFAALKTKNNGKAL
                     FLNDANMAHYIIDYKGALLTDLLSEVESLENVTKFQILQDRKLLAKAGVISYADVVNI
                     LPSFTNEESYLVNTGLSQLISELELFVDEDSETEKAFQSLVGKLFAKNYARLGWDKVA
                     GESAGDESLRGIVLSKTLYSENADAKTKASQIFAAHKENLASIPADIRPIVLNNEIKT
                     TNSAELVKTYRETYIKTSLQEFKRELEGAVALIKDEKVIAELLESFKNADIVKPQDIA
                     FSWFYLLRNDFSQDAAWAWEKANWAFLEEKLGGDMSYDKFVIYPGNTFKTADKLAEYK
                     AFFEPKLENQGLKRSIEMAIKQITARVALIDSQKAAVDKAITDIAEKL"
BASE COUNT     1049 a    515 c    652 g    984 t
ORIGIN      
        1 aagcttatgg tcttgtttta aatgatggac cactcaaggg ccgtcttgct cgtagcgttt
       61 ttgttgtaaa agaggggcaa atcgtctata gtgaaatatt agcggacgtt tcggatgagc
      121 ctaattacga aaaagcctta gaagcaacta aataattaat gacaaaaaat tgaggatatt
      181 gataagagtg aatatcctct gtaaaagctg tcagtagaca gtttttttaa taagttaaag
      241 aaaagatgta atttttcttt gtactcgaaa ttttctattc aatttgatat aattatatta
      301 atactgaata tttaggagaa gatatggctg taaaacgttt aattgaaact tttgttccgg
      361 aaaattataa aattttcctt gatattgacc gtaaaactaa gaaaataaaa ggtcaagtgg
      421 caattactgg ggaagcaaaa gatactgttg tttcctttca taccaaagga ttacacttca
      481 acaaagttcg cgcttttagt gttgatacaa acttcattga aaatgaagaa gatgaagaaa
      541 ttgttgttaa aattggtgaa acagggcgcg tgactgtttc atttgaatat gaagccgaat
      601 tgactgacaa catgatggga atctatcctt catattatga agtaaatggt gaaaagaaaa
      661 tgctaattgg cacacagttt gaaagccatt ttgctcgcca agctttccca tctattgatg
      721 aaccagaagc gaaagcaact ttcgatttgt cagtaaaatt tgatgaagaa gaaggcgaca
      781 tcattgtttc aaatatgcca gaactcttga atattaatgg aattcacgtt tttgaacgta
      841 ctgttaaaat gagttcttac cttttggctt ttgtattcgg tgaacttcaa tataaaaaag
      901 gaaaaacaaa atctggtgtt gaagtaggtg cctttgcaac gaaagctcat agtcaagcag
      961 cacttgattt cccacttgat attgctattc gttcaatcga attttacgaa gattattatc
     1021 aaacacccta tccacttcca cacagctggc atattgcctt gcctgacttc tcagcaggag
     1081 caatggaaaa ctggggatgt atcacttatc gtgaagtttg catgttggtt gaccctgaaa
     1141 atgcaaccat tcaaagtaaa caatatgtgg caacagttat tgcccatgaa ttggctcacc
     1201 aatggttcgg tgaccttgta actatgcaat ggtgggatga tttgtggctc aacgaatcat
     1261 tcgctaacaa catggaatat gtttgtatgg atgctttgga accaagttgg aacgtttggg
     1321 aatcattctc aatctcagaa gccaatatgg cattgaatcg tgatgcaact gacggagttc
     1381 aatctgtcca cgttgaagta actcacccag atgaaattgg aacacttttt gatccagcaa
     1441 tcgtctatgc taaaggttca cgcttaatgg ttatgcttcg taaatggctc ggagatgaag
     1501 actttgctgc tggcttagcc ctttatttca aacgccatca atatggaaat acagttggag
     1561 acaatctttg ggatgccttg gctgaagttt ctggaaaaga tgtggcagcc ttcatgcact
     1621 catgggttaa tcaaccgggt taccctgtcg ttacagcaga agtcgttgat gatactttga
     1681 ttttgagtca aaaacaattc tttgttggtg aaggtgttga caaaggacgt ttgtggaatg
     1741 ttccattgaa taccaattgg acagggctac cagacttgct ttcttctgaa aaagttgaaa
     1801 ttccagggtt tgctgctttg aaaactaaaa acaatggtaa agctctcttt ttgaatgatg
     1861 caaatatggc tcattacatc attgattata agggtgcttt actgacagac cttctttcag
     1921 aagttgaatc tttggagaat gtgacaaaat tccaaatctt gcaagaccgt aaattgttgg
     1981 ctaaagcagg tgtgatttct tatgctgacg ttgtaaatat cttgccatca ttcactaatg
     2041 aagaatctta ccttgttaat actggtttga gtcaactcat tagtgagttg gaactttttg
     2101 ttgatgaaga ttcagaaact gaaaaagcct tccaaagctt ggtaggaaaa ctttttgcta
     2161 aaaattatgc tcgtttgggt tgggataaag ttgctggtga atctgctggg gatgagagcc
     2221 ttcgtggaat tgtgttgagt aaaactttgt attctgaaaa tgcagatgcc aaaacaaaag
     2281 ctagccaaat ttttgcagca cataaagaaa atttagcaag tattccagct gatattcgtc
     2341 caattgtttt gaacaatgaa atcaaaacaa ctaactcggc tgaattggtt aaaacttatc
     2401 gtgaaactta tatcaaaaca agcctccaag aattcaaacg tgaacttgaa ggagcagttg
     2461 ctttgattaa agatgaaaaa gttattgctg aattacttga aagcttcaaa aatgcagata
     2521 ttgttaaacc acaagatatt gctttctctt ggttctacct cttacgaaat gatttttcac
     2581 aagatgcagc atgggcttgg gaaaaagcaa attgggcttt ccttgaagaa aaattgggtg
     2641 gtgatatgag ttatgacaaa tttgtcatct atccagggaa tactttcaaa actgctgata
     2701 aattagcgga atataaagct ttctttgaac caaaattaga aaaccaaggc ttgaaacgtt
     2761 caattgaaat ggcaattaaa caaattacag cacgtgttgc tttgattgat agccaaaaag
     2821 cagcagttga taaagcaatt actgatattg ctgaaaaatt gtaatttata aataaaaatc
     2881 accttttaga ggtggttttt ttatttataa attattcgtt tgatttcgct ttcgatagaa
     2941 caatcaaagc gagaataagg aacataaatc ccataagggc gggagcagaa tgtccgagac
     3001 taatgtaaat ttgtccacca attaaaggac cgataacgcg tgtaagtgct tgaagagctt
     3061 gacttccgcc ttgaagttta ccttgttcag aagaagtcgc tgattttgaa agtttgccca
     3121 ttgaaggcag ttccgaaaat actatctcca aaagcaaaga taaacattgc cagaatgaag
     3181 agggccacat tttagaaaag
//