From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: O.volvulus antigen (pot. cysteine proteinase inhibitor) mRNA,
Date: 15 Sep 92 10:04:59 GMT
Distribution: bionet
Checksum: 08951     4

LOCUS       ONGOV7        719 bp ss-mRNA            INV       15-SEP-1992
DEFINITION  O.volvulus antigen (pot. cysteine proteinase inhibitor) mRNA,
            complete cds.
ACCESSION   M37105
KEYWORDS    antigen; cysteine proteinase inhibitor.
SOURCE      O.volvulus (strain Savanna) adult worm, cDNA to mRNA, (library of
            J.Donelson), clone OV7.
  ORGANISM  Onchocerca volvulus
            Eukaryota; Animalia; Metazoa; Arthropoda; Uniramia; Insecta;
            Pterygota; Neoptera; Holometabola; Diptera; Nematocera; Culicoidea;
            Similiidae.
REFERENCE   1  (bases 1 to 719)
  AUTHORS   Lustigman,S., Brotman,B., Huima,T. and Price,A.M.
  TITLE     Characterization of an Onchocerca volulus cDNA clone encoding a
            genus specific antigen present in infective larvae and adult worms
  JOURNAL   Mol. Biochem. Parasitol. 45, 65-75 (1991)
  STANDARD  simple staff_entry
REFERENCE   2  (bases 1 to 719)
  AUTHORS   Lustigman,S., Brotman,B., Huima,T., Prince,A.M. and McKerrow,J.H.
  TITLE     Molecular cloning and characterization of onchocystatin, a cysteine
            proteinase inhibitor of Onchocerca volvulus
  JOURNAL   J. Biol. Chem. 267, 17339-17346 (1992)
  STANDARD  simple staff_entry
COMMENT     Draft entry and computer-readable sequence for [Unpublished (1990)
            Lindsley F. Kimball Res Inst, The New York BloodCtr, 310 E. 6]
            kindly submitted
            by S.Lustigman, 25-JUL-1990.
FEATURES             Location/Qualifiers
     CDS             62..550
                     /product="cysteine proteinase inhibitor"
                     /note="putative"
                     /codon_start=1
                     /translation="MLTIKDGTLLIHLLLFSVVALVQLQGAKSARAKNPSKMESKTGE
                     NQDRPVLLGGWEDRDPKDEEILELLPSILMKVNEQSNDEYHLMPIKLLKVSSQVVAGV
                     KYKMDVQVARSQCKKSSNEKVDLTKCKKLEGHPEKVMTLEVWEKPWENFMRVEILGTK
                     EV"
     sig_peptide     62..157
                     /codon_start=1
     mat_peptide     158..547
                     /product="cysteine proteinase inhibitor"
                     /note="putative"
                     /codon_start=1
     polyA_signal    550..555
                     /note="putative"
     polyA_signal    692..697
                     /note="putative"
     polyA_site      719
BASE COUNT      242 a    105 c    155 g    217 t
ORIGIN
        1 ggtttaatta cccaagtttg aggaatctgc tgagtaaaca aagaggctta gcgacacgat
       61 aatgttgaca ataaaggatg gaacgttgct catacattta ttgctgttca gtgtggtagc
      121 attagttcag ttgcaaggag ccaagtctgc aagagccaaa aatccgtcaa aaatggagtc
      181 caaaacaggt gaaaatcaag atcgtcccgt tttattggga ggttgggaag atcgcgatcc
      241 aaaggatgaa gaaatcctgg aactattgcc aagcatattg atgaaagtaa atgaacaatc
      301 aaacgatgaa tatcatttga tgccgatcaa attactgaag gtttcatctc aagttgtcgc
      361 tggtgtgaaa tacaagatgg atgtgcaggt tgctcgatcg caatgtaaaa aaagttcgaa
      421 tgaaaaagtt gatctaacaa agtgcaaaaa attagaagga catcctgaaa aggttatgac
      481 tttggaagtt tgggagaaac catgggagaa ttttatgcgc gtcgaaattc tgggaacaaa
      541 agaagtatga ataaaattct ttctgtagtt tttttctcca ctctatttta gacttcttct
      601 ctggttcttt gctaaaacat ttctgtcgtt ttggtgcctt agatattttc gattatattg
      661 aataattttg taaaatccaa ggtaatcttt taattaatgc ataaagattt aaagctgat
//