From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: hsp83A; Pharbitis nil; ; 3563 base-pairs.
Date: 20 Aug 92 10:04:15 GMT
Distribution: bionet
Checksum: 60047     8

LOCUS       PHNHSP83A    3563 bp ds-DNA             PLN       20-AUG-1992
DEFINITION  hsp83A; Pharbitis nil; ; 3563 base-pairs.
ACCESSION   M99431
KEYWORDS    heat shock protein 83.
SOURCE      Pharbitis nil (strain Violet) DNA.
  ORGANISM  Pharbitis nil
            Eukaryota; Plantae; Embryobionta; Magnoliophyta; Magnoliopsida;
            Asteridae; Solanales; Convolvulaceae.
REFERENCE   1  (bases 1 to 3563)
  AUTHORS   Felsheim,R.F. and Das,A.
  TITLE     Structure and expression of a heat shock protein 83 (hsp83) gene of
            Pharbitis nil
  JOURNAL   Plant Physiol. (1992) In press
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     CDS             join(534..813,1254..1534,1636..3186)
                     /product="heat shock protein 83"
                     /gene="Hsp83A"
                     /label=cds
                     /codon_start=1
                     /translation="MADVQMAEAETFAFQAEINQLLSLIINTFYSNKEIFLRELISNA
                     SDALDKIRFESLTDKSKLDAQPELFIRLVPDKTNKTLSIIDSGVGMAKADLVNNLGTI
                     ARSGTKEFMEALQAGADVSMIGQFGVGFYSAYLVAEKVIVTTKHNDDEQYIWESQAGG
                     SFTVTRDVDGEQLGRGTKITLFLKEDQLEYLEERRIKDLVKKHSEFISYPIYLWTEKT
                     TEKEISDDEDDEPKKEEEGDIEEVDEDKEKEGKKKKKIKEVSHEWQLINKQKPIWLRK
                     PEEITKEEYASFYKSLTNDWEDHLAVKHFSVEGQLEFKAILFVPKRAPFDLFDTRKKM
                     NNIKLYVRRVFIMDNCEELIPEYLGFVKGVVDSDDLPLNISREMLQQNKILKVIRKNL
                     VKKCIEMFNEIAENKDDYNKFYEAFSKNLKLGIHEDSQNRAKLADLLRYYSTKSGDEL
                     TSLKDYVTRMKEGQKDIYYITGESKKAVENSPFLERLKKKGYEVLFMVDAIDEYAVGQ
                     LKEYDGKKLVSATKEGLKLEDDDEEEKKKREEKKKSFENLCKIIKDILGDKVEKVVVS
                     DRIVDSPCCLVTGEYGWTANMERIMKAQALRDSSMSSYMSSKKTMEINPDNGIMEELR
                     KRAEADKNDKSVKDLVLLLFETALLTSGFSLDDPNTFGARIHRMLKLGLSIDEEEAGD
                     DADMPALEEEAGEESKMEEVD"
     promoter        147..161
                     /note="alternate"
                     /gene="Hsp83A"
     promoter        290..309
                     /note="alternate"
                     /gene="Hsp83A"
     promoter        340..364
                     /note="alternate"
                     /gene="Hsp83A"
     TATA_signal     387
                     /note="Putative"
                     /gene="Hsp83A"
     exon            423..813
                     /number=1
                     /gene="Hsp83A"
     exon            1254..1534
                     /number=2
                     /gene="Hsp83A"
                     /label=exon2
     exon            1636..>3186
                     /number=3
                     /gene="Hsp83A"
                     /label=exon3
     polyA_signal    3515..3519
                     /note="putative"
                     /gene="Hsp83A"
BASE COUNT     1087 a    618 c    818 g   1040 t
ORIGIN
        1 tataaaaaaa attatattca aaatttatat aaaaaatatt aataaaaata taatttaatt
       61 aataaataat aaatagtaca aataaaatag acaatcataa ttctaataga ttacatatag
      121 gtacgcgtac gccggcccca acaacatgaa cattcgagat tttcatagaa gtatgatttc
      181 ccgcactaat ttatcatcag cattattaaa cgacatcgtt ccataggtgg atccaatcaa
      241 actttaaaaa taacggcgtg gagaagaagt cgagtgtctg gagggtagta gaatactcca
      301 gaagcttgag aaatccaata tttttcgcct tcaactttcc ttcgccaata gaaggaccta
      361 gaatacactc gttttcccta ctcgcgtata taaacacacc actcttcgtt tcaattagag
      421 tcatcaggac aaaagcggtt atacgtcgtt ctcgatctct ctgcgctttc tttctttgcg
      481 tttctaagat cttccccgcg agcgttcata aaggctacag agttctgtga tcaatggcgg
      541 atgttcagat ggccgaggcg gagaccttcg cattccaggc ggagatcaac cagctgctca
      601 gcttgatcat caacacgttc tacagcaaca aggagatttt cctccgtgag ctcattagca
      661 acgcctctga cgccttggat aagatccggt ttgagagctt gactgacaag agcaagctcg
      721 atgctcagcc tgagctcttc attcgccttg ttcctgacaa gactaacaag actctctcca
      781 ttattgatag cggtgtcggc atggccaaag caggtaccag attgcttcct tctcgtattt
      841 tttttttcgt atatgattac gtttttgttg ctctgttttg tttatggtaa atactgtgga
      901 attgtgatga ctttacatgt ctataaatat gcttctgttt agcacatgtt gtctctaaca
      961 ataatgccga tatcgctgtt gaattttgat atactgttaa tgtattatgt atttgcactg
     1021 atttacgcac ataaacatat gatgtgacgt tacacatgaa taatgctagt gttgtggagt
     1081 tttgatgata atgcatatgc acttacatga tccccatctc tgctatggta catttaagtg
     1141 ctcttcctgt gaagatatgt taaaatttat tgagatgaat cttgtgggaa gcatatactc
     1201 tagtgtactt ggaacagtca taatgatttg ttttatgctg tggtcatgta cagatttggt
     1261 gaacaacttg ggtacaattg cgagatcagg aaccaaggaa tttatggaag cattgcaggc
     1321 tggtgccgat gtgagcatga tcgggcaatt tggtgtgggt ttctactcag cataccttgt
     1381 tgcagagaag gtcattgtga ccaccaaaca caatgatgac gaacagtaca tctgggaatc
     1441 acaagctggt ggttcattca ctgtcactag ggatgtggat ggagagcaac tgggcagagg
     1501 aaccaagatt actctcttcc tcaaggaaga ccaggtaatt ctggttatgc aattaaattt
     1561 agctataatg tatatatata gtttgctttc tgattggcta aaaattgcta tgtatgtctt
     1621 tgaatattgt gccagcttga atacttggag gaaaggagaa tcaaggacct tgtgaagaag
     1681 cattctgaat tcattagcta cccaatctac ctctggactg agaagacaac cgagaaggaa
     1741 atcagtgatg atgaggatga tgagcccaag aaggaagagg agggtgacat tgaagaggtg
     1801 gatgaggaca aagagaagga gggtaagaag aagaagaaga tcaaggaggt ttctcatgag
     1861 tggcagctca ttaacaagca aaaaccaatc tggttgcgca agcctgagga gatcaccaag
     1921 gaggaatatg cctctttcta caagagttta accaatgatt gggaggatca tcttgctgtc
     1981 aagcacttct ctgttgaggg tcagcttgaa ttcaaggcca tcctatttgt tccaaagagg
     2041 gcaccatttg atctttttga cacccgcaag aagatgaaca acattaagct gtatgtcagg
     2101 agggtcttta tcatggacaa ttgtgaggaa ctcatccctg agtacctagg atttgtgaag
     2161 ggtgttgttg actctgatga tttgcccctc aacatttctc gtgagatgtt gcaacagaat
     2221 aagatcctca aggtgatcag gaagaacctt gtcaagaaat gcattgagat gttcaatgag
     2281 attgctgaga acaaggatga ctacaacaag ttctatgaag cattctcaaa gaatttgaag
     2341 ctaggaattc atgaagacag ccagaacagg gcaaaattgg cagacttgct gcgttactac
     2401 tccactaaga gtggtgatga gttgacaagc ctaaaggact atgtcactag gatgaaagag
     2461 ggtcaaaaag acatctacta catcactggt gagagcaaaa aagctgttga aaattcacca
     2521 ttcctggagc gcctcaagaa gaagggttat gaagtactgt ttatggttga tgccattgac
     2581 gagtatgctg taggccaatt gaaggagtat gatggtaaga aactggtgtc agccaccaag
     2641 gaagggctga aacttgaaga cgatgatgaa gaagagaaga agaagcggga ggagaagaag
     2701 aaatcttttg agaacctttg caagatcatc aaagatattc tgggtgacaa agtcgagaag
     2761 gtggttgtct ctgacaggat tgttgattca ccctgctgct tggtgactgg tgagtatgga
     2821 tggacagcaa atatggaaag gatcatgaag gctcaggccc taagggacag cagcatgagc
     2881 tcttacatgt ctagcaagaa aaccatggaa attaaccctg acaatggaat catggaggag
     2941 cttagaaaga gagcagaggc tgataagaat gacaaatcag tgaaggacct tgtgttgctg
     3001 ttgtttgaga ctgcactcct tacatccggt ttcagtcttg atgatcccaa cacttttgga
     3061 gcaagaattc acaggatgct gaagttgggt ctcagcattg atgaggagga agctggtgat
     3121 gatgctgaca tgcctgcatt ggaggaagaa gctggtgagg aaagcaagat ggaagaagtg
     3181 gactaaggga catatcttat ttcttcattt gtaacaaaag ctatattgag tcaatgagtc
     3241 cttttcctta gtgtcttctg gctgtgttat gtatgaattt tcattgcagt cattgtatgt
     3301 attcgggaag tgcaatacag ttgcgttttg gattttcctt cttttttttt ttttttgctg
     3361 aaaaagcctt catctctctt ttccaactga aatttatttg aagagtaagg tcttgacggc
     3421 aaattatagc atggaacatg gtctatttta tattgtagac tctagtataa aaattaacct
     3481 tcaaattttg ttgcattgta gactcaggtt taaaaataac cttcaaattt tgtatttata
     3541 gttaatatat tttatagcag ttg
//