From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: C.acetobutylicum ThrA gene encoding Thr-tRNA.
Date: 11 Sep 92 10:03:09 GMT
Distribution: bionet
Checksum: 22323     4

LOCUS       CLOTHRAG     1645 bp ds-DNA             BCT       11-SEP-1992
DEFINITION  C.acetobutylicum ThrA gene encoding Thr-tRNA.
ACCESSION   X65276
KEYWORDS    heat shock protein; heat shock protein 18; hsp18 gene; thrA gene;
            transfer RNA-Thr.
SOURCE      Clostridium acetobutylicum DNA.
  ORGANISM  Clostridium acetobutylicum
            Prokaryotae; Firmicutes; Endospore-forming rods and cocci;
            Bacillaceae.
REFERENCE   1  (bases 1 to 1645)
  AUTHORS   Sauer,U. and Drre,P.
  TITLE     Possible function of tRNA-Thr ACG in regulation of solvent
            formation of Clostridium acetobutylicum
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
REFERENCE   2  (bases 1 to 1645)
  AUTHORS   Sauer,U.
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
COMMENT     *source: strain=DSM 792;
            From EMBL    entry CATHRAG;  dated 03-SEP-1992.
FEATURES             Location/Qualifiers
     terminator      complement(176..204)
     CDS             complement(305..760)
                     /product="heat shockprotein"
                     /gene="hsp18"
                     /label=hsp18
                     /codon_start=1
                     /translation="MFGMVPFRRNNNGLMRREDFFDKMFDNFFSDDFFPTTTFNGNAG
                     FKVDIKEDDDKYTVAADLPGVKKDNIELQYENNYLTINAKRDDIVETKDDNNNFVRRE
                     RSYGELRRSFYVDNIDDSKIDASFLDGVLRITLPKKVKGKDNGRRIDIH"
     RBS             complement(769..773)
     -10_signal      complement(916..921)
     -35_signal      complement(940..945)
     tRNA            1141..1216
                     /anticodon=(pos:1174..1176,aa:thr)
                     /product="transfer RNA-Thr"
                     /gene="ThrA"
     terminator      1227..1259
BASE COUNT      599 a    225 c    246 g    575 t
ORIGIN
        1 gatcttgtta tgttccttta tagagatgaa tattacaata aggaaactga acaaaagaat
       61 gttgcagaat gcataatagc aaaacagaga aatggtccta caggaactgt taatcttgct
      121 tggcttggtc agtacagtaa gtttggtagg ttagatgtaa ttcatcaaga ataataaaaa
      181 acaacttgtg gataacacaa gttgtttttt atattttata gttattaaca ttgttaatat
      241 aaaaatcata ctactgtgga taagtagggg gaagaaagtt atcaacagtg tataactttt
      301 ttacttagtg gatatcaatt cttcttccat tatcttttcc cttaaccttc ttaggtaaag
      361 ttattctaag aactccatca agaaaagatg catcaatttt tgagtcatca atattatcta
      421 cataaaagct tcgtctaagt tctccataac ttctttcacg tcttacaaaa ttattgttat
      481 catctttagt ctcaacaatg tcatctcttt ttgcatttat agttaaatag ttgttttcgt
      541 attgaagctc aatgttatct tttttaacac caggtaaatc tgctgcaact gtatatttat
      601 catcatcttc ttttatatct actttgaagc ctgcatttcc attgaatgta gtagtaggga
      661 aaaagtcatc tgagaaaaag ttatcaaaca tcttatcgaa aaaatcctct cttctcatta
      721 aaccattatt atttcttcta aaaggaacca ttccaaacat aattaaaacc tcctaatatt
      781 aaatattttt tagttattag cttattttaa ttttttattc aatgtataca ttaaaggtgc
      841 aaagcgaatt gaatcaaaat atttttagaa aatactgaat gtttgaagtt tttcttctct
      901 tcaccttaca agtatatgat agtacgaaag tcaaagaaag tcaaaatcaa aaaggatttt
      961 aaatattaag attttaggag gtattcaaag cgattgtggg ataaatttac tttttcaaaa
     1021 aatataaaaa gttttagtag catttcatca gaaataaaaa ttacaatgtt agaacttcaa
     1081 tttttttcat aaaaagttgt gtaaaaggga ttaatgtagt aaaatatatt tcgtaagtat
     1141 gctcctgtag cgcagttggt agcgcagctg attcgtaatc agttggtcgt aggttcaaat
     1201 cctatctgga gcaccaagag agttaatgcc aaagctttta gagctttggc atttttttat
     1261 gtggtgaaaa gccccaatat aaaatgtaaa aggtaactga atttctacct aatatattta
     1321 tatataatac aatacgtaaa gcaactttta gggatttaca caaaaagaca atattaatta
     1381 tttcgttata attatcaata agatgtaaac tgttgaaaac ccttgaaata aaccattgac
     1441 agtcgaagat gagagtaata taataaatct gcaatgggaa aagtttggta atgaatacga
     1501 atggtattaa atttcgtatt taactgtagc aaacaatacc aaagatatat ataaggagga
     1561 gttattatgg ctcaaatatc tgtaacacct gaagagttaa aatctcaagc tcaggtttat
     1621 attcaatcta aagaagaaat cgatc
//