From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: ZAP-70=70 kda protein-tyrosine kinase [human, T-cells Jurkat, mRNA,
Date: 27 Jan 93 05:55:22 GMT
Distribution: bionet
Checksum: 01587 6

LOCUS       S117721      2454 bp    mRNA            PRI       12-JAN-1993
DEFINITION  ZAP-70=70 kda protein-tyrosine kinase [human, T-cells Jurkat, mRNA,
            2454 nt]
ACCESSION   S48685 L05148
KEYWORDS    .
SOURCE      human T-cells Jurkat
  ORGANISM  Unclassified.
            Unclassified.
REFERENCE   1  (bases 1 to 2454)
  AUTHORS   Chan,A.C., Iwashima,M., Turck,C.W. and Weiss,A.
  TITLE     ZAP-70: a 70 kd protein-tyrosine kinase that associates with the
            TCR zeta chain.
  JOURNAL   Cell 71, 649-662 (1992)
  STANDARD  full automatic
COMMENT     This entry [NCBI gibbsq 117721] was created by the journal scanning
            component of NCBI/GenBank at the National Library of Medicine.
            This sequence comes from Fig. 3.
FEATURES             Location/Qualifiers
     CDS             210..2069
                     /note="mismatch(527[C->V]).  70 kda protein-tyrosine
                     kinase;  For the protein sequence (NCBI gibbsq 117722):
                     Method: conceptual translation with partial peptide
                     sequencing.  This sequence comes from Fig. 3.
                     Author-given protein sequence is in conflict with the
                     conceptual translation"
                     /product="ZAP-70"
                     /codon_start=1
                     /translation="MPDPAAHLPFFYGSISRAEAEEHLKLAGMADGLFLLRQCLRSLG
                     GYVLSLVHDVRFHHFPIERQLNGTYAIAGGKAHCGPAELCEFYSRDPDGLPCNLRKPC
                     NRPSGLEPQPGVFDCLRDAMVRDYVRQTWKLEGEALEQAIISQAPQVEKLIATTAHER
                     MPWYHSSLTREEAERKLYSGAQTDGKFLLRPRKEQGTYALSLIYGKTVYHYLISQDKA
                     GKYCIPEGTKFDTLWQLVEYLKLKADGLIYCLKEACPNSSASNASGAAAPTLPAHPST
                     LTHPQRRIDTLNSDGYTPEPARITSPDKPRPMPMDTSVYESPYSDPEELKDKKLFLKR
                     DNLLIADIELGCGNFGSVRQGVYRMRKKQIDVAIKVLKQGTEKADTEEMMREAQIMHQ
                     LDNPYIVRLIGVCQAEALMLVMEMAGGGPLHKFLVGKREEIPVSNVAELLHQVSMGMK
                     YLEEKNFVHRDLAARNVLLVNRHYAKISDFGLSKALGADDSYYTARSAGKWPLKWYAP
                     ECINFRKFSSRSDVWSYGCTMWEALSYGQKPYKKMKGPEVMAFIEQGKRMECPPECPP
                     ELYALMSDCWIYKWEDRPDFLTVEQRMRACYYSLASKVEGPPGSTQKAEAACA"
BASE COUNT      499 a    782 c    761 g    412 t
ORIGIN      
        1 ggaataggtt agtttcagac aagcctgctt gccggagctc agcagacacc aggccttccg
       61 ggcaggcctg gcccaccgtg ggcctcagag ctgctgctgg ggcattcaga accggctctc
      121 cattggcatt gggaccagag accccgcaag tggcctgttt gcctggacat ccacctgtac
      181 gtccccaggt ttcgggaggc ccaggggcga tgccagaccc cgcggcgcac ctgcccttct
      241 tctacggcag catctcgcgt gccgaggccg aggagcacct gaagctggcg ggcatggcgg
      301 acgggctctt cctgctgcgc cagtgcctgc gctcgctggg cggctatgtg ctgtcgctcg
      361 tgcacgatgt gcgcttccac cactttccca tcgagcgcca gctcaacggc acctacgcca
      421 ttgccggcgg caaagcgcac tgtggaccgg cagagctctg cgagttctac tcgcgcgacc
      481 ccgacgggct gccctgcaac ctgcgcaagc cgtgcaaccg gccgtcgggc ctcgagccgc
      541 agccgggggt cttcgactgc ctgcgagacg ccatggtgcg tgactacgtg cgccagacgt
      601 ggaagctgga gggcgaggcc ctggagcagg ccatcatcag ccaggccccg caggtggaga
      661 agctcattgc tacgacggcc cacgagcgga tgccctggta ccacagcagc ctgacgcgtg
      721 aggaggccga gcgcaaactt tactctgggg cgcagaccga cggcaagttc ctgctgaggc
      781 cgcggaagga gcagggcaca tacgccctgt ccctcatcta tgggaagacg gtgtaccact
      841 acctcatcag ccaagacaag gcgggcaagt actgcattcc cgagggcacc aagtttgaca
      901 cgctctggca gctggtggag tatctgaagc tgaaggcgga cgggctcatc tactgcctga
      961 aggaggcctg ccccaacagc agtgccagca acgcctcagg ggctgctgct cccacactcc
     1021 cagcccaccc atccacgttg actcatcctc agagacgaat cgacaccctc aactcagatg
     1081 gatacacccc tgagccagca cgcataacgt ccccagacaa accgcggccg atgcccatgg
     1141 acacgagcgt gtatgagagc ccctacagcg acccagagga gctcaaggac aagaagctct
     1201 tcctgaagcg cgataacctc ctcatagctg acattgaact tggctgcggc aactttggct
     1261 cagtgcgcca gggcgtgtac cgcatgcgca agaagcagat cgacgtggcc atcaaggtgc
     1321 tgaagcaggg cacggagaag gcagacacgg aagagatgat gcgcgaggcg cagatcatgc
     1381 accagctgga caacccctac atcgtgcggc tcattggcgt ctgccaggcc gaggccctca
     1441 tgctggtcat ggagatggct gggggcgggc cgctgcacaa gttcctggtc ggcaagaggg
     1501 aggagatccc tgtgagcaat gtggccgagc tgctgcacca ggtgtccatg gggatgaagt
     1561 acctggagga gaagaacttt gtgcaccgtg acctggcggc ccgcaacgtc ctgctggtta
     1621 accggcacta cgccaagatc agcgactttg gcctctccaa agcactgggt gccgacgaca
     1681 gctactacac tgcccgctca gcagggaagt ggccgctcaa gtggtacgca cccgaatgca
     1741 tcaacttccg caagttctcc agccgcagcg atgtctggag ctatggggtc accatgtggg
     1801 aggccttgtc ctacggccag aagccctaca agaagatgaa agggccggag gtcatggcct
     1861 tcatcgagca gggcaagcgg atggagtgcc caccagagtg tccacccgaa ctgtacgcac
     1921 tcatgagtga ctgctggatc tacaagtggg aggatcgccc cgacttcctg accgtggagc
     1981 agcgcatgcg agcctgttac tacagcctgg ccagcaaggt ggaagggccc ccaggcagca
     2041 cacagaaggc tgaggctgcc tgtgcctgag ctcccgctgc ccaggggagc cctccacgcc
     2101 ggctcttccc caccctcagc cccaccccag gtcctgcagt ctggctgagc cctgcttggt
     2161 tgtctccaca cacagctggg ctgtggtagg gggtgtctca ggccacaccg gccttgcatt
     2221 gcctgcctgg ccccctgtcc tctctggctg gggagcaggg aggtccggga gggtgcggct
     2281 gtgcagcctg tcctgggctg gtggctcccg gagggccctg agctgagggc attgcttaca
     2341 cggatgcctt cccctgggcc ctgacattgg agcctgggca tcctcaggtg gtcaggcgta
     2401 gatcaccaga ataaacccag cttccctctt gaaaaaaaaa aaaaaaaaaa aacc
//