From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human (clones SW11-1 and cKi-ras2-76) cellular transforming
Date: 31 Jul 92 10:01:36 GMT
Distribution: bionet
Checksum: 62905     4

LOCUS       HUMRASK04     615 bp ds-DNA             PRI       31-JUL-1992
DEFINITION  Human (clones SW11-1 and cKi-ras2-76) cellular transforming
            c-Ki-ras2 proto-oncogene, exon 3.
ACCESSION   L00047 K00652 K00653
KEYWORDS    K-ras oncogene; c-myc oncogene; proto-oncogene; ras oncogene;
            transforming gene.
SEGMENT     4 of 6
SOURCE      Homo sapiens (library: Lawn et al.) lung DNA; and Homo sapiens
            colon and lung cDNA to mRNA.
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 299 to 458)
  AUTHORS   Shimizu,K., Birnbaum,D., Ruley,M.A., Fasano,O., Suard,Y.,
            Edlund,L., Taparowsky,E., Goldfarb,M. and Wigler,M.
  TITLE     Structure of the Ki-ras gene of the human lung carcinoma cell line
            Calu-1
  JOURNAL   Nature 304, 497-500 (1983)
  STANDARD  full staff_review
REFERENCE   2  (bases 1 to 615)
  AUTHORS   McGrath,J.P., Capon,D.J., Smith,D.H., Chen,E.Y., Seeburg,P.H.,
            Goeddel,D.V. and Levinson,A.D.
  TITLE     Structure and organization of the human Ki-ras proto-oncogene and a
            related processed pseudogene
  JOURNAL   Nature 304, 501-506 (1983)
  STANDARD  full staff_review
REFERENCE   3  (bases 299 to 458)
  AUTHORS   Capon,D.J., Seeburg,P.H., McGrath,J.P., Hayflick,J.S., Edman,U.,
            Levinson,A.D. and Goeddel,D.V.
  TITLE     Activation of Ki-ras2 gene in human colon and lung carcinomas by
            two different point mutations
  JOURNAL   Nature 304, 507-513 (1983)
  STANDARD  full staff_review
REFERENCE   4  (bases 299 to 458)
  AUTHORS   McCoy,M.S., Bargmann,C.I. and Weinberg,R.A.
  TITLE     Human colon carcinoma Ki-ras2 oncogene and its corresponding
            proto-oncogene
  JOURNAL   Mol. Cell. Biol. 4, 1577-1582 (1984)
  STANDARD  full staff_review
COMMENT     See comment for segment 1 of this entry.
            Complete source information:
            Human DNA (genomic library of Lawn et al.) [2]; human cDNA to colon
            (SW480) and lung (Calu-1) carcinoma cell lines mRNA, multiple
            clones [3]; human lung carcinoma cell line Calu-1 DNA [1]; human
            colon carcinoma cell line SW480, cDNA to mRNA, clone SW11-1, and
            human cell line GM637, cDNA to mRNA, clone cKi-ras2-76 [4]
FEATURES             Location/Qualifiers
     intron          order(L00046:537..>789,<1..298)
                     /number=C
                     /note="1.1 kb gap"
                     /gene="c-Ki-ras2"
     exon            299..458
                     /number=3
                     /gene="c-Ki-ras2"
     conflict        374
                     /gene="c-Ki-ras2"
                     /citation=[4]
BASE COUNT      211 a     81 c    108 g    215 t
ORIGIN
        1 tctagaattt ttcagtagtt tctgttttac tattatgatc tacctgcata ttaacctatt
       61 aggttatagt tttactatac ttctaggtat ttgatctttt gagagagata caaggtttct
      121 gtttaaaaag gtaaagaaac aaaataacta gtagaagaag gaaggaaaat ttggtgtagt
      181 ggaaactagg aattacattg ttttctttca gccaaatttt atgacaaaag ttgtggacag
      241 gttttgaaag atatttgtgt tactaatgac tgtgctataa cttttttttc tttcccagag
      301 aacaaattaa aagagttaag gactctgaag atgtacctat ggtcctagta ggaaataaat
      361 gtgatttgcc ttctagaaca gtagacacaa aacaggctca ggacttagca agaagttatg
      421 gaattccttt tattgaaaca tcagcaaaga caagacaggt aagtaacact gaaataaata
      481 cagatctgtt ttctgcaaaa tcataactgt tatgtcattt aatatatcag tttttctctc
      541 aattatgcta tactaggaaa taaaacaata tttagtaaat gtttttgtct cttgagaggg
      601 cattgcttct taatc
//