From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Plasmid pWR100 (from Shigella flexneri) intercellular spread gene
Date: 24 Jul 92 10:10:46 GMT
Distribution: bionet
Checksum: 59270     8

LOCUS       W10ICSB      3164 bp ds-DNA             BCT       24-JUL-1992
DEFINITION  Plasmid pWR100 (from Shigella flexneri) intercellular spread gene
            (icsB) and ipgA, ipgB, ipgC genes, complete cds.
ACCESSION   M86530
KEYWORDS    antigen; icsB gene; intercellular spread; ipaB gene; ipgA gene;
            ipgB gene; ipgC gene; periplasmic protein.
SOURCE      Plasmid pWR100 DNA.
  ORGANISM  Plasmid pWR100
            Prokaryotae.
REFERENCE   1  (sites)
  AUTHORS   Baudry,B., Kaczorek,M. and Sansonetti,P.J.
  TITLE     Nucleotide sequence of the invasion plasmid antigen B and C genes
            (ipaB and ipaC) of Shigella flexneri
  JOURNAL   Microb. Pathog. 4, 345-357 (1988)
  STANDARD  full staff_review
REFERENCE   2  (bases 1 to 3164)
  AUTHORS   Allaoui,A., Mounier,J., Prevost,M.C., Sansonetti,P.J. and
            Parsot,C.R.
  TITLE     icsB: A Shigella flexneri virulence gene necessary for the lysis of
            protrusions during intercellular spread
  JOURNAL   Mol. Microbiol. 6, 1605-1616 (1992)
  STANDARD  full staff_entry
FEATURES             Location/Qualifiers
     CDS             101..1585
                     /function="involved in intercellular spread"
                     /gene="icsB"
                     /codon_start=1
                     /translation="MSLKISNFIDASNTKGPIRVEDTEHGPILIAQKFNLKDLFFRTL
                     STINAKINSQILNEQLKNYRLENQKSLLLFLNTLASEKSAESAFAAYEAAKNSIQHSF
                     TGRDIKLMLNTAEAFHGIGTAKNLERHLVFRCWGNRGITHLGHTSISIKNNLLQEPTH
                     TYLSWYPGGNVTKDTEINYLFEKRSGYSVDTYKQDKLNMISEQTAERLDAGQEVRNLL
                     NSKQDQNNNKKIFFPRANQKKDPYGYWGVSADKVYIPLSGDNKTKDGKISHNLFGLDE
                     TNMSKFICKKKADAFRQLANYKLISKSENCAGMALNVLKAGNSEIYFPLPDVKLVATP
                     NDVYAYANKVRQRIESLNQSYNEIMKYIESDFDLSRLTQLRRSYLKSFNKINLIHTPK
                     TFKPLSISLYKHPTENVSSEDFDAVINACHSYLVKSAPSNMTRVLNELKTEATDKKEE
                     IIEKSIKIIDYYNSLKSPDLGTKLYIHDLLQINKLLLNNSHSNI"
     CDS             1598..1987
                     /gene="ipgA"
                     /codon_start=1
                     /translation="MCRKLYDKLYEITGAKLDFNDKNQAFILLEEQIPVCITDNDEYI
                     FLTGLLNEHELFTENIINPEHILILNYSLSRDYGSSICLLPDTHQCVLTKKHYKKYLS
                     PDELIESLYEFLFCIKLTIANITSEVN"
     CDS             2003..2629
                     /gene="ipgB"
                     /codon_start=1
                     /translation="MQILNKILPQVEFAIPRPSFDSLSRNKLVKKILSVFNLKQRFPQ
                     KNFGCPVNINKIRDSVIDKIKDSNSGNQLFCWMSQERTTYVSSMINRSIDEMAIHNGV
                     VLTSDNKKNIFAAIEKKFPDIKLDEKSAQTSISHTALNEIASSGLRAKILKRYSSDMD
                     LFNTQMKDLTNLVSSSVYDKIFNESTKVLQIEISAEVLKAVYRQSNTN"
     CDS             2685..3153
                     /gene="ipgC"
                     /codon_start=2
                     /translation="MSLNITENESISTAVIDAINSGATLKDINAIPDDMMDDIYSYAY
                     DFYNKGRIEEAEVFFRFLCIYDFYNVDYIMGLAAIYQIKEQFQQAADLYAVAFALGKN
                     DYTPVFHTGQCQLRLKAPLKAKECFELVIQHSNDEKLKIKAQSYLDAIQDIKE"
     CDS             3159..>3164
                     /gene="ipaB"
                     /codon_start=1
                     /translation="MH"
BASE COUNT     1205 a    569 c    486 g    904 t
ORIGIN
        1 gttaacaggc tcaagtttct aaatttatta attgaatata ttatatacca aatacatttt
       61 acatccccac aatcaccaag taatggagag ttaataaagt atgagcctca aaattagcaa
      121 tttcattgac gcaagcaata caaaagggcc tatacgcgtt gaagatacag agcatggacc
      181 tatattgata gcacagaaat tcaacctaaa ggatctcttt ttcagaacat taagcactat
      241 taatgcaaag ataaattcac agatattaaa tgaacaacta aaaaattatc gactagagaa
      301 tcaaaaatcc ttattgcttt tcctcaatac attagctagc gaaaaatctg cagaaagtgc
      361 gtttgccgcc tatgaagcag ctaaaaactc aattcaacac tctttcacag gtagagacat
      421 caaattaatg ttgaataccg cagaggcttt tcatggcatc ggtacagcca aaaatcttga
      481 aagacattta gtttttcgtt gctggggaaa tagaggcata acccatttag ggcatactag
      541 tatcagtata aaaaacaatt tactacagga accaactcat acctatctca gctggtatcc
      601 cggcgggaat gttacaaaag atacagaaat aaactacctt tttgaaaaac gctcaggtta
      661 cagtgtcgat acctataagc aagacaaact aaatatgatt tcagagcaaa cagccgaaag
      721 acttgatgca ggccaagagg ttagaaactt attaaattca aaacaagatc aaaataacaa
      781 caaaaaaata ttttttccca gagctaatca aaaaaaagac ccctatggtt attggggggt
      841 cagtgctgat aaagtataca ttccattatc aggtgataat aaaacaaagg atggtaaaat
      901 cagccataac ctttttggcc ttgatgaaac aaacatgtca aaatttatat gcaaaaagaa
      961 agcagatgct ttcagacaat tggcaaatta taaattaatc agtaaatctg aaaactgtgc
     1021 tggtatggca cttaatgttc ttaaagcagg aaattctgaa atatactttc cactccctga
     1081 cgttaaattg gttgctacac ctaacgatgt atacgcgtat gcaaataaag ttaggcaacg
     1141 cattgaaagt ctcaatcagt cttataacga aataatgaag tatattgaat ctgattttga
     1201 tctttccaga ttgactcaat tacgacgcag ctatcttaaa agtttcaata aaattaacct
     1261 tatccacaca ccaaaaacat tcaagccttt atcaatttca ctttacaaac atcctactga
     1321 aaatgtatct tcagaagact ttgatgcggt catcaacgct tgccactcat acttagtaaa
     1381 atcagcaccc agcaatatga ctcgtgtatt aaacgaatta aaaaccgaag caacagataa
     1441 aaaagaagaa atcatcgaaa aatcaataaa aattattgat tattacaatt ctcttaaatc
     1501 gccagatctt ggaaccaaac tttacattca cgacttatta cagatcaata agcttttatt
     1561 gaataactct cattctaata tatagaaggc catagaaatg tgtcgcaaac tatatgataa
     1621 actttatgaa ataacaggtg caaagcttga ttttaacgat aaaaatcaag cctttatatt
     1681 gctcgaagaa caaatacctg tttgtataac agataatgat gaatacatct ttttgaccgg
     1741 acttcttaat gaacatgaac tttttacaga aaatattatt aatccagaac acatactcat
     1801 ccttaactac tctttgagta gagattatgg ttcttctatc tgtcttctcc ctgatactca
     1861 tcaatgtgtt ttgaccaaga aacactacaa aaaatatctt tcacctgatg aactcattga
     1921 atctttgtat gaatttttat tttgtattaa actaaccatt gcaaacatca cttcagaagt
     1981 gaactaacat atagggggta tcatgcaaat tctaaacaaa atacttccac aggttgaatt
     2041 cgcgattcct cgcccatcat ttgattcgtt aagtcgcaat aagcttgtaa agaaaatatt
     2101 atctgttttt aacttaaaac aacgctttcc tcagaagaat tttggatgcc cggtgaatat
     2161 aaataaaatc agggacagcg tcatagacaa aataaaagat agtaattcgg ggaatcaact
     2221 attttgttgg atgagccaag agcgaaccac ttatgtctct tcaatgataa acagaagtat
     2281 agatgaaatg gccatacaca atggagttgt tttgacctca gataacaaaa aaaacatatt
     2341 tgcagctatt gaaaaaaaat tcccggatat aaaacttgat gaaaaatcag cacaaacatc
     2401 tatcagtcat acagcactaa acgagattgc ctcatctggc ctcagagcaa agattttaaa
     2461 acgctatagc agtgacatgg atttgtttaa cacacaaatg aaggatctta caaatctagt
     2521 atccagttcc gtctacgata aaatatttaa tgaatcaaca aaagttttac aaatagaaat
     2581 aagcgcagag gttctaaagg ctgtataccg tcaaagcaat acaaattaaa tcttttatat
     2641 taaatcttat acttattaaa gtaaagcaga caaaaaggag accttatgtc tttaaatatc
     2701 accgaaaatg aaagcatctc tactgcagta attgatgcaa ttaactctgg cgctacactg
     2761 aaagatatta atgcaattcc tgatgatatg atggatgaca tttattcata tgcttatgac
     2821 ttttacaaca aaggaagaat agaggaagct gaagttttct tcaggttttt atgtatatac
     2881 gacttttaca atgtagacta cattatggga ctcgcagcta tttatcagat aaaagaacag
     2941 ttccaacaag cagcagacct ttatgctgtc gcttttgcat taggaaaaaa tgactataca
     3001 ccagtattcc atactggaca atgtcagctt cggttgaaag cccccttaaa agctaaagag
     3061 tgcttcgaac tcgtaattca acacagcaat gatgaaaaat taaaaataaa agcacaatca
     3121 tacttggacg caattcagga tatcaaggag taattattat gcat
//