From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: D.pseudoobscura Adh gene for alcohol dehydrogenase
Date: 28 Dec 92 21:26:53 GMT
Distribution: bionet
Checksum: 50933 8

LOCUS       DROADHGN27   3467 bp ds-DNA             INV       01-DEC-1992
DEFINITION  D.pseudoobscura Adh gene for alcohol dehydrogenase
ACCESSION   X62207
KEYWORDS    ADH gene; alcohol dehydrogenase.
SOURCE      Drosophila pseudoobscura DNA.
  ORGANISM  Drosophila pseudoobscura
            Eukaryota; Animalia; Metazoa; Arthropoda; Uniramia; Insecta;
            Pterygota; Neoptera; Holometabola; Diptera; Brachycera;
            Cyclorrhapha; Schizophora; Drosophiloidea; Drosophilidae.
REFERENCE   1  (bases 1 to 3467)
  AUTHORS   Schaeffer,S.W. and Miller,E.L.
  TITLE     Estimates of gene flow in Drosophila pseudoobscura determined from
            nucleotide sequence analysis of the alcohol dehydrogenase region
  JOURNAL   Genetics 132, 471-480 (1992)
  STANDARD  full automatic
COMMENT     *source: strain=GB54; *source: isolate=Gundlach Bundschu Winery,
            Sonoma Valley, *source: California; *source: chromosome=IV section
            88;
               From EMBL    entry DPADHGN27;  dated 16-OCT-1992.
FEATURES             Location/Qualifiers
     mRNA            join(40..119,912..1017,1082..1486,1547..1962)
                     /note="adult mRNA (Adh)"
     mRNA            join(879..1017,1082..1486,1547..1962)
                     /note="larval mRNA (Adh)"
     promoter        8..14
                     /note="adult promoter (Adh)"
     prim_transcript 40..1962
                     /note="adult primary transcript (Adh)"
     exon            40..119
                     /note="adult exon 1a"
     intron          120..911
                     /note="adult intron"
     promoter        843..850
                     /note="larval promoter (Adh)"
     prim_transcript 879..1962
                     /note="larval primary transcript (Adh)"
     exon            879..1017
                     /note="larval exon 1"
     exon            912..1017
                     /note="adult exon 1b"
     intron          1018..1081
                     /number=1
     exon            1082..1486
                     /number=2
     intron          1487..1546
                     /number=2
     exon            1547..1962
                     /number=3
     polyA_signal    1939..1944
     polyA_site      1962
     CDS             join(925..1017,1082..1486,1547..1813)
                     /EC_number="1.1.1.1"
                     /gene="Adh"
                     /product="alcoholdehydrogenase"
                     /codon_start=1
                     /translation="MSLTNKNVVFVAGLGGIGLDTSRELVKRNLKNLVILDRIDNPAA
                     IAELKAINPKVTITFYPYDVTVPVAETTKLLKTIFAQVKTIDVLINGAGILDDHQIER
                     TIAVNYTGLVNTTTAILDFWDKRKGGPGGIICNIGSVTGFNAIYQVPVYSGSKAAVVN
                     FTSSLAKLAPITGVTAYTVNPGITKTTLVHKFNSWLDVEPRVAEKLLEHPTQTSQQCA
                     ENFVKAIELNKNGAIWKLDLGTLEPITWTQHWDSGI"
BASE COUNT      972 a    788 c    794 g    913 t
ORIGIN      
        1 cgtagtgtat ttaaggcact gcacatgtcg aggaccacga ttattgtcta cagtgcagtc
       61 agtagttgtc aggtgcagca ggcggaccag catttcttta aatctactca atcgatcaag
      121 taagtaactg taatccaagt acagtcggcc catcccactc ggttctgccg taactcaaga
      181 aatttcttga tagcgcagtt ccagttgtaa aacgtcgttt aaaaaaacat ctcgttagag
      241 cttgggttga aaaacgttcg agttgggcgt agaaaagttc aaatataaaa tatcaaaaag
      301 agaaaatatc ttcccaaagc agctgtccat tttttgttaa agtgatagat caaaaattcg
      361 tatgacgtat ttgccaagtg ctctttatgc aaataatttc ctcaattcta attctacaag
      421 aagcactttt ggctcttgtc cagaaaaaac aatttgttta cacttgtcgg atgataacat
      481 aagcggaccg acggctaagt aacataacag tcgcctgtta agtaatgccc tcactcgctg
      541 ttagatggcc gcgcgtgtcg actccgatag accagcagac acgcagtagt tttcctcaag
      601 aacctgaact ctaaacatag acataattta cgataccttg agactcaaat aaaaatacaa
      661 gtatgagtgg tattctattc ctgattagac tcgcacacac acatacgagt attaacataa
      721 atttgccggc aaataaccaa aacaaaacga acgcaaacga tccgaacacg taatgcgaga
      781 gataagaaac agaaaagctc gacgtgaaag aagcttctgg cggaagctct tgccgaactc
      841 cctataaata cgagacagaa aaccagaata aatataataa caaactgttg aattgtccca
      901 tcgattccca gatcaaaaga cagaatgtct ctcaccaaca agaacgtcgt tttcgtggcc
      961 ggtctgggcg gcattggcct ggacaccagc cgggagttgg tcaagcgtaa tctgaaggta
     1021 agagagaacg aattccatgg aatctatgga aatcctaaat ttaaaaattc atttatttta
     1081 gaacctggtc atcctggatc gcattgacaa cccggctgcc attgccgagc tgaaggcaat
     1141 caatcccaag gtgaccatca ccttctatcc ctacgatgtg actgtgcccg tcgctgagac
     1201 caccaagctc ctgaagacca tctttgccca ggtgaagaca atcgatgtcc tgatcaacgg
     1261 tgctggcatc ctggacgatc atcagatcga gcgcaccatt gccgttaact acacgggcct
     1321 ggtcaacacc accacagcca ttctggactt ctgggacaag cgcaagggcg gcccaggcgg
     1381 catcatttgc aacattggct ccgtcaccgg tttcaatgcc atctaccagg tgcccgttta
     1441 ctccggctcc aaggcggcgg tggttaactt tacctcctcc ctggcggtaa gtaatccaac
     1501 gtttttccta ctccccaaaa ctaaatattt ctttgaaatc ccatagaaac tggctcccat
     1561 tactggtgtc actgcttaca ctgtcaatcc tggcatcact aagaccactc tggtccacaa
     1621 attcaactcg tggctggatg tggagccccg tgtggcagag aagctgctcg agcatcccac
     1681 ccagacctct cagcagtgcg ccgagaactt tgtgaaggcc attgagctga acaagaacgg
     1741 tgccatctgg aagttggatt tgggcacctt ggagcccatc acatggaccc agcactggga
     1801 ctcgggcatc taaaccatcc cagagactct atgggacatg gcgttttagc ttttagtttc
     1861 tttttttcca ctcaattgtt acgtatatac atacacatat ggcaataagg ctgatttgaa
     1921 ccccgttttg aatatgataa tacaaaatta tatttgagaa atttcaacaa aatggataag
     1981 agctctagct ctcttttaat taaaaaaaac tagataaaaa gagcaatggt cagtggtggt
     2041 cagtggtcgt tgtggcctct atcgatttcc acacaaaaac tttactgttt agtaatagaa
     2101 aagaacttga gaaaggcagc caaaagaatg tacgatctga cgggtaagca tgtctgctat
     2161 gtggctgact gcggtggcat tgcactggag accagcaagg ttctcatgac caagaatata
     2221 gcggtgagtg gtagagaggt ggtgtagaga gtggagagtg gttctggttc ttaaacaagt
     2281 ctctagtctc tagtctcttg gctgctgctg gtagctgtcg aaactaatta agttaatgac
     2341 tttttcatgt tttgtttttg tccgctgact tttgctgttc aaagctacaa ttaagtttat
     2401 gttttgagtt tgatctactt ttgcttaagg gaagtacttt cgattacgga ttgctggcat
     2461 ataaaaatag ataccaagga atgtgcctca tatcatattg attctatctt gattctttgc
     2521 ccagaaactg gccatccttc agagcgtgga aaaccccccg gccatcgctc agttacaatc
     2581 gattaagcac agcacacaga tcttcttctg gaccttcgat gtgaccatgg cccgcgagga
     2641 gatgaagaag tacttcgatg aggtcatggt ccagatggac tacatcgatg tgctgatcaa
     2701 tggggccacc ctgtgcgatg agcggaacat tgatgccacc atcaatacga atctgaccgg
     2761 catgatgaac accgtggcca ctgtgctgcc ctatatggac cgaaagatgg gcggttcggg
     2821 tggactgatt gtgaatgtca cctccgtcat aggactggat ccatcgccag tcttttgtgc
     2881 gtacagtgcc tcaaagtttg gtgtgattgg gttcacccga agtctagcgg tgagtgggcg
     2941 agatcttaac ttgaaaatgc tctctaatgt cttccattct ttcgcaggat cccctgtatt
     3001 acacccaaaa tggtgtggct gtcatggccg tctgctgtgg tcccaccaaa gtgtttgtcg
     3061 atcgcgaact gactgccttc ctggaatacg gccagtcctt tgccgatcgc ctgcgtcgtg
     3121 ccccctgcca atcgacggcc gtctgcggca agaatatagt taatgccatc gagagatcgg
     3181 agaacggaca gatctggatt gccgacaagg gcggcctgga gtcggtggcc ctccactggt
     3241 attggcatat ggccgatcag tttgtgaact atatgcagag tactgacgat gaggatcagg
     3301 agtttttttt gggccagcga tgaggcatgc cttgatccac ggaataaaac atcaaatgtg
     3361 tcacaaaaag taggcaactt gtttgtgggt ttggaaatag atttgaaata ttttgaggtc
     3421 atatgaataa tgacataaat gagagtgcct gaaagtatgc caagttt
//