From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: P. fluorescens gene for arylesterase, complete cds
Date: 15 Sep 92 10:05:08 GMT
Distribution: bionet
Checksum: 57777     4

LOCUS       PSEARLE      1196 bp ds-DNA             BCT       15-SEP-1992
DEFINITION  P. fluorescens gene for arylesterase, complete cds
ACCESSION   D12484 D01134
KEYWORDS    aryl-ester hydrolase; arylesterase.
SOURCE      Pseudomonas fluorescens (strain SIK WI) (library: pUC19) DNA, clone
            pUE1251.
  ORGANISM  Pseudomonas fluorescens
            Prokaryotae; Gracilicutes; Scotobacteria; Pseudomonadaceae.
REFERENCE   1  (bases 1 to 1196)
  AUTHORS   Choi,K.D., Jeohn,G.H., Rhee,J.S. and Yoo,O.J.
  TITLE     Cloning and nucleotide sequence of an esterase gene from
            Pseudomonas fluorescens and expression of the gene in Escherichia
            coli
  JOURNAL   Agric. Biol. Chem. 54, 2039-2045 (1992)
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     -35_signal      158..164
     -10_signal      176..181
     RBS             236..240
     CDS             248..958
                     /EC_number="3.1.1.2"
                     /product="arylesterase"
                     /codon_start=1
                     /translation="MSTFVAKDGTQIYFKDWGSGKPVLFSHGWPLDADMWEYQMEYLS
                     SRGYRTIAFDRPVWPLGPTLDRQRLRHLRRRHRPVDRTPGPQGGDPGGLLHGRRRCGP
                     LHRPPRQRTGGRPGAAGRRHPAVRPEARLSAGCPVDVFARFKTELLKDRAQFISDFNA
                     PFYGINKGQVVSQGVQTQTLQIALLASLKATVDCVTGSPKPTSARTWPRSTYPPWYPW
                     RWRLRSCRSRPPAKWRRS"
     repeat_region   1041..1061
                     /rpt_type=inverted
                     /rpt_unit=1041..1048
                     /rpt_unit=1054..1061
BASE COUNT      225 a    378 c    357 g    236 t
ORIGIN
        1 gatctggacg tcctcggccc ccggcgcgcg gcgtcgaagg tgaagggctt gagggcatcc
       61 ttggcgttct gggcggcgta gctgtaagtc ttggccattt cgttcacctg tgtgatctga
      121 cggtagaggt cagtggacca gcatagacgc tgaaccgttc aaccgagcac accggtacaa
      181 gcccttcgtc cggtttcacc gaatagtgcg ctagagtctt tcagccccct caaccaggag
      241 tttttgcatg agcacatttg ttgcaaaaga cggtacccag atctatttca aggactgggg
      301 cagcggtaaa ccggtgttgt tcagccacgg ttggccactg gatgccgaca tgtgggaata
      361 ccagatggag tacctcagca gccgcggcta tcgcaccatc gcctttgacc ggccggtttg
      421 gccgctcgga ccaaccctgg accggcaacg actacgacac cttcgccgac gacatcgccc
      481 agttgatcga acacctggac ctcaaggagg tgaccctggt gggcttctcc atgggcggcg
      541 gcgatgtggc ccgctacatc gcccgccacg gcagcgcacg ggtggccggc ctggtgctgc
      601 tgggcgccgt caccccgctg ttcggccaga agcccgacta tccgcagggt gtcccgtcga
      661 tgtgttcgca aggttcaaga ctgagctgct gaaggatcgc gcgcagttca tcagcgattt
      721 caacgcaccg ttctatggca tcaacaaggg ccaggtcgtc tcccaaggcg tgcagaccca
      781 gaccctgcaa atcgccctgc tggcctcgct caaggccacg gtggattgcg tcaccggttc
      841 gccgaaaccg acttccgccc ggacatggcc aagatcgacg tacccaccct ggtatccatg
      901 gcgatggcga ctcagatcgt gccgttcgag accaccggca aagtggcggc ggagttgatc
      961 aagggcgccg aactgaaggt gtacaaggag ggccccacgg gttcgcggtg acccacgccc
     1021 agcagttgaa cgaagacctg ttggcgttct tgaaacgctg agttaatgtc tgcctttatc
     1081 ggctgggctt gcccggccct ttttttacgc gctcgaatct cgcgcggtcg ccgggcacga
     1141 tcgtgctgaa ccgccgcccc ctcgccacgg tcccaaccca tacgttcatc gctgca
//