From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Bradyrhizobium japonicum cytochrome P-450 BJ-1 (P450-BJ1) gene,
Date: 29 Sep 92 10:08:40 GMT
Distribution: bionet
Checksum: 20029     4

LOCUS       RHBP450A     1379 bp ds-DNA             BCT       29-SEP-1992
DEFINITION  Bradyrhizobium japonicum cytochrome P-450 BJ-1 (P450-BJ1) gene,
            complete cds.
ACCESSION   L02323
KEYWORDS    P450-BJ1 gene; cytochrome P-450 BJ-1; heme binding site;
            ribosome binding site.
SOURCE      Bradyrhizobium japonicum (library: USDA 110) DNA.
  ORGANISM  Bradyrhizobium japonicum
            Prokaryotae; Gracilicutes; Scotobacteria; Rhizobiaceae.
REFERENCE   1  (bases 1 to 1379)
  AUTHORS   Tully,R.E. and Keister,D.L.
  TITLE     Cloning, sequencing, and mutagenesis of a cytochrome P-450 gene
            from Bradyrhizobium japonicum
  JOURNAL   Unpublished (1992)
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     RBS             21..24
                     /gene="P450-BJ1"
                     /note="putative"
                     /citation=[1]
     CDS             32..1237
                     /standard_name="cytochrome P-450"
                     /product="cytochrome P-450 BJ-1"
                     /gene="P450-BJ1"
                     /note="putative"
                     /codon_start=1
                     /translation="MSEQQPLPTLPMWRVDHIEPSPEMLALRANGPIHRVRFPSGHEG
                     WWVTGYDEAKAVLSDAAFRPAGMPPAAFTPDSVILGSPGWLVSHEGREHARLRAIVAP
                     AFSDRRVKLLVQQVEAIAAHLFETLAAQPQPADLRRHLSFPLPAMVISALMGVLYEDH
                     AFFAGLSDEVMTHQHESGPRSASRLAWEELRAYIRGKMRDKRQDPDDNLLTDLLAAVD
                     QGKASEEEAVGLAAGMLVAGHESTVAQIEFGLHAMFRHPQQRERLVGDPSLVDKAVQE
                     ILRMYPPGAGWDGIMRYPRTDVTIAGEHIPAESKVLVGLPATSFDPHHFDDPEIFDIE
                     RQEKPHLAFSYGPHACIGVALARLELKVVFGSIFQRLPALRLAVAPEQLKLRKEIITG
                     GFEQFPVLW"
                     /citation=[1]
     misc_binding    1058..1087
                     /bound_moiety="P-450-type heme"
                     /gene="P450-BJ1"
                     /note="putative"
                     /citation=[1]
BASE COUNT      231 a    456 c    462 g    230 t
ORIGIN
        1 ccttccatcg cttagagagt gaagtgccac catgtccgaa caacaaccct tgccgacgct
       61 gccgatgtgg cgcgtcgatc acatcgagcc ctcgcccgag atgttggcgc tacgcgccaa
      121 cggtccgatc caccgcgtgc gcttcccctc cgggcacgaa ggctggtggg tgacaggcta
      181 tgacgaggcc aaggcggtgc tgtccgacgc ggcgttccgg cccgcgggaa tgccgccggc
      241 ggcattcacc ccggattcgg tgattctcgg ttcgccgggg tggctggtct cgcacgaggg
      301 gcgcgagcat gcccggctac gcgcgatcgt ggcgccggcc ttcagtgacc gcagggtgaa
      361 gctgctcgtg cagcaggtcg aagcgatcgc cgcgcacttg ttcgagacgc tggcggccca
      421 gccccagccc gccgacctgc ggcgccacct ctcctttccg cttccggcca tggtcatcag
      481 cgcgctgatg ggcgtgctct acgaggatca cgcctttttc gccgggctgt ccgatgaggt
      541 gatgacgcac cagcatgaaa gcggcccgcg cagcgcgtcc cgcctggcct gggaagaact
      601 gcgcgcctac attcgcggca agatgcgcga caagcgccag gatccggacg acaacctgct
      661 gacggatctg ctcgctgcgg tcgaccaggg caaggcgagc gaggaagagg cggttggcct
      721 ggcggcgggc atgctggtgg cggggcacga gagcaccgtc gcgcagatcg aattcggcct
      781 gcatgccatg ttccgccatc cgcaacagcg cgaacgcctg gtcggcgatc cgtccctggt
      841 cgacaaggcg gtacaggaaa tcctgcgcat gtacccgccg ggcgcgggct gggatggcat
      901 catgcgctat ccgaggaccg acgtgaccat cgcgggcgag catattcccg cggagagcaa
      961 ggtgctggtc ggcctcccgg caacgtcgtt cgatccgcac catttcgacg atccggaaat
     1021 cttcgatatc gaacgccagg aaaagccgca cctggctttt tcctacgggc cgcacgcgtg
     1081 catcggcgtg gcgctggcca ggctggagct caaggtggtg ttcggttcga tcttccagcg
     1141 ccttcccgcg ctgcgcctgg ccgtggcgcc ggaacaactg aagttgcgca aggagatcat
     1201 cacgggcggg ttcgagcagt tcccggtgct ctggtgatgt cgcggacgcc acgggcgatc
     1261 tcgatcttct ccggaatctg ccgcgcgcct gtcgcgcgcc ggtcagatca gcccgccaac
     1321 aggtaaccag gatggacgtg caagaaacca cggcagcatg ccgggacgcc ttcgccgaa
//