From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Nematode choline acetyltransferase (cha-1) mRNA exons 1-11,
Date: 26 Jan 93 05:46:38 GMT
Distribution: bionet
Checksum: 64232 8

LOCUS       CELCHA1X     2081 bp ss-mRNA            INV       25-JAN-1993
DEFINITION  Nematode choline acetyltransferase (cha-1) mRNA exons 1-11,
            complete cds.
ACCESSION   L08969
KEYWORDS    choline acetyltransferase.
SOURCE      Caenorhabditis elegans (strain Bristol N2) cDNA to mRNA.
  ORGANISM  Caenorhabditis elegans
            Eukaryota; Animalia; Eumetazoa; Nematoda; Secernentea; Rhabditida;
            Rhabditina; Rhabditoidea; Rhabditidae.
REFERENCE   1  (bases 1 to 2081)
  AUTHORS   Alfonso,A., Grundahl,K., McManus,J.R. and Rand,J.B.
  TITLE     Cloning and characterization of the choline acetyltransferase
            structural gene (cha-1) from C. elegans
  JOURNAL   Unpublished (1993)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     misc_RNA        <1..9
                     /function="trans-spliced onto many C. elegans mRNAs"
                     /standard_name="SL1"
                     /gene="cha-1"
                     /evidence="EXPERIMENTAL"
                     /citation=[1]
                     /note="these are the last 9 nucleotides of the
                     22-nucleotide spliced leader SL1"
     exon            10..75
                     /number=1
                     /gene="cha-1"
                     /citation=[1]
                     /note="putative"
     exon            76..126
                     /number=2
                     /gene="cha-1"
                     /citation=[1]
                     /note="putative"
     exon            127..176
                     /number=3
                     /gene="cha-1"
                     /citation=[1]
                     /note="putative"
     exon            177..318
                     /number=4
                     /gene="cha-1"
                     /citation=[1]
                     /note="putative"
     exon            319..491
                     /number=5
                     /gene="cha-1"
                     /citation=[1]
                     /note="putative"
     exon            492..768
                     /number=6
                     /gene="cha-1"
                     /citation=[1]
                     /note="putative"
     exon            769..937
                     /number=7
                     /gene="cha-1"
                     /citation=[1]
                     /note="putative"
     exon            938..1179
                     /number=8
                     /gene="cha-1"
                     /citation=[1]
                     /note="putative"
     exon            1180..1403
                     /number=9
                     /gene="cha-1"
                     /citation=[1]
                     /note="putative"
     exon            1404..1602
                     /number=10
                     /gene="cha-1"
                     /citation=[1]
                     /note="putative"
     exon            1603..2081
                     /number=11
                     /gene="cha-1"
                     /citation=[1]
                     /note="putative"
     polyA_site      2081
                     /evidence="EXPERIMENTAL"
                     /citation=[1]
                     /note="RM#50P terminates after residue #2078 (no polyA);
                     RM#17L ispolyadenylated following residue #2079; RM#69P
                     ispolyadenylated following residue 2081"
     CDS             76..1959
                     /function="synthesis of acetylcholine"
                     /EC_number="2.3.1.6"
                     /standard_name="ChAT"
                     /gene="cha-1"
                     /note="putative"
                     /product="choline acetyltransferase"
                     /codon_start=1
                     /translation="MEKEKVDELPPNDNWYETALPKPPVPSLEATLDRYLEYAAVVAV
                     GQKASLATTHDAAHKFVRQATPLQEQLLEIAEKSPNWATKFWLPEMYMRVRMPTPVNS
                     NPGYIFPKVKFETKEDHIKYTALLTRGLLEYKNLIDTKQVCREKSTGAQKLQMCMEQY
                     DRVLSCYREPGVGEDTQIRKQKTNDGNEHVLVMCRNQTFLLHSRINGALVSYADVEYQ
                     LAQIEEISKINQNNTANIGASGVGPRDNAALFWQDMLTVEQNSKSYEWVKSALFVVCL
                     DMEDPIDYGKNDTMSISEKEKEFVARGYSTLTGHGSSKFGLNRWYDATIQLVVSSSGV
                     NGLCIEHSTAEGIVIINMAETAIRYAQKYFKSKMVWNDVRNVHPKSLTWHFSENSRNI
                     LKKQAEVFDELANELELEVLIFNEFGKDSIKNWRVSPDGFIQLIMQLAHYKTHGHLVS
                     TYESASVRRFGAGRVDNIRANTQEALEWVTAMASKKESKERKLELFKKAVLKQVKVTL
                     ENISGYGVDNHLCALFCLAREREETTGEDIPSLFLDPLWSEVMRFPLSTSQVTTSLDI
                     PDCYLTYGAVVRDGYGCPYNIQPDRVIFAPTAFRSDPRTDLQHFKKSLAGAMRDVKEL
                     LSN"
BASE COUNT      601 a    418 c    533 g    529 t
ORIGIN      
        1 aagtttgagg taattcatcg aaagtctttc tattttccgc atctcttgtt caaaatagta
       61 acctactaaa acaagatgga aaaggaaaaa gttgacgaac ttccaccaaa tgacaattgg
      121 tatgagactg cactgccaaa gccgccggta ccaagcctgg aggccacgct agatagatat
      181 ctcgaatacg ctgccgttgt agccgtaggc cagaaagcct cactagcgac aacccacgat
      241 gcagctcaca agttcgtgcg tcaagctact ccgttacagg agcaactgct ggaaattgcg
      301 gagaagtcgc cgaattgggc cacaaaattt tggctccccg agatgtatat gagagttagg
      361 atgccaacgc ccgtgaattc aaatccgggg tatatttttc cgaaagtcaa atttgagaca
      421 aaggaggatc acatcaaata tacggcattg ctgacaagag ggctcttgga gtacaagaac
      481 ttgatagaca caaaacaagt gtgccgagaa aagtccactg gcgcgcaaaa actccagatg
      541 tgcatggaac agtacgaccg agttctcagc tgctacaggg agccgggcgt cggcgaggat
      601 acccaaatac gaaagcagaa gacgaatgat gggaatgaac atgtactcgt gatgtgccgg
      661 aatcagactt ttttgctcca ttcgcgaatc aatggagctc tagtgtcata tgcagatgtg
      721 gagtaccagc tggcgcagat cgaggagatt tctaagatta atcaaaacaa caccgcaaat
      781 atcggagcct ctggcgttgg cccgcgtgat aacgcggctc ttttttggca ggatatgctc
      841 acagtcgagc agaactcaaa atcctatgaa tgggtaaagt cggcgctttt cgtggtgtgt
      901 cttgacatgg aagatcctat tgattatggg aaaaatgata ccatgagcat ttccgagaag
      961 gagaaggaat ttgtggcgcg tggctactca actctgaccg gccatggaag ttcaaagttc
     1021 gggctcaatc ggtggtatga tgcgactatt cagctggtgg tgtcttccag tggtgtgaat
     1081 gggttgtgca tagagcactc gacggccgag gggattgtga ttattaatat ggctgaaact
     1141 gctatcagat atgcacagaa gtactttaaa agcaagatgg tttggaacga cgtgcgcaat
     1201 gtacacccca aatctctgac atggcatttt agtgaaaatt ctagaaatat tctgaaaaag
     1261 caggcggaag ttttcgatga gctcgccaac gaactggagc ttgaagtttt gattttcaac
     1321 gaattcggca aagattccat caaaaattgg cgtgtcagtc cggatggatt tattcagttg
     1381 attatgcaat tggcgcatta taaaacacat ggtcacctcg tttccaccta cgaatcagct
     1441 tcggttcggc gattcggtgc gggaagagtt gataacattc gggcgaacac acaagaggcg
     1501 ctggaatggg ttacggcaat ggcgagcaag aaggagtcta aagagagaaa gctggaatta
     1561 tttaagaagg ctgtgctcaa gcaggtcaag gtcacgttgg agaacatcag cggctacggt
     1621 gtcgacaatc atctttgcgc cctattctgt ctcgcccggg aaagggaaga aacgaccgga
     1681 gaagatatac catcactgtt tctggatcct ctgtggtctg aagttatgcg attcccattg
     1741 tcgacaagcc aagtcacaac ctctctggat atccctgatt gctacctgac gtatggtgca
     1801 gtggtccgcg atggctatgg atgcccgtac aatattcaac cggatcgagt gatttttgcg
     1861 ccgactgcgt tcagaagtga tccgaggact gatttacaac attttaagaa aagcttggct
     1921 ggagctatga gagatgtcaa ggagctgctg agcaactgaa ttttattatt attttttgag
     1981 atatttttta tttaatttgg caataatttg aatatagaga tgcatgtagt tgagtgctca
     2041 gaattttttc ccttaattgg aaataaagtg gatttgtata t
//