From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: D.pseudoobscura Adh gene for alcohol dehydrogenase
Date: 28 Dec 92 21:27:36 GMT
Distribution: bionet
Checksum: 27508 8

LOCUS       DROADHGN57   3470 bp ds-DNA             INV       01-DEC-1992
DEFINITION  D.pseudoobscura Adh gene for alcohol dehydrogenase
ACCESSION   X62237
KEYWORDS    ADH gene; alcohol dehydrogenase.
SOURCE      Drosophila pseudoobscura DNA.
  ORGANISM  Drosophila pseudoobscura
            Eukaryota; Animalia; Metazoa; Arthropoda; Uniramia; Insecta;
            Pterygota; Neoptera; Holometabola; Diptera; Brachycera;
            Cyclorrhapha; Schizophora; Drosophiloidea; Drosophilidae.
REFERENCE   1  (bases 1 to 3470)
  AUTHORS   Schaeffer,S.W. and Miller,E.L.
  TITLE     Estimates of gene flow in Drosophila pseudoobscura determined from
            nucleotide sequence analysis of the alcohol dehydrogenase region
  JOURNAL   Genetics 132, 471-480 (1992)
  STANDARD  full automatic
COMMENT     *source: strain=SB2; *source: isolate=San Bernadino Mountains,
            California; *source: chromosome=IV section 88;
               From EMBL    entry DPADHGN57;  dated 16-OCT-1992.
FEATURES             Location/Qualifiers
     mRNA            join(40..119,915..1020,1084..1488,1549..1964)
                     /note="adult mRNA (Adh)"
     mRNA            join(882..1020,1084..1488,1549..1964)
                     /note="larval mRNA (Adh)"
     promoter        8..14
                     /note="adult promoter (Adh)"
     prim_transcript 40..1964
                     /note="adult primary transcript (Adh)"
     exon            40..119
                     /note="adult exon 1a"
     intron          120..914
                     /note="adult intron"
     promoter        846..853
                     /note="larval promoter (Adh)"
     prim_transcript 882..1964
                     /note="larval primary transcript (Adh)"
     exon            882..1020
                     /note="larval exon 1"
     exon            915..1020
                     /note="adult exon 1b"
     intron          1021..1083
                     /number=1
     exon            1084..1488
                     /number=2
     intron          1489..1548
                     /number=2
     exon            1549..1964
                     /number=3
     polyA_signal    1941..1946
     polyA_site      1964
     CDS             join(928..1020,1084..1488,1549..1815)
                     /EC_number="1.1.1.1"
                     /gene="Adh"
                     /product="alcoholdehydrogenase"
                     /codon_start=1
                     /translation="MSLTNKNVVFVAGLGGIGLDTSRELVKRNLKNLVILDRIDNPAA
                     IAELKAINPKVTITFYPYDVTVPVAETTKLLKTIFAQVKTIDVLINGAGILDDHQIER
                     TIAVNYTGLVNTTTAILDFWDKRKGGPGGIICNIGSVTGFNAIYQVPVYSGSKAAVVN
                     FTSSLAKLAPITGVTAYTVNPGITKTTLVHKFNSWLDVEPRVAEKLLEHPTQTSQQCA
                     ENFVKAIELNKNGAIWKLDLGTLEPITWTQHWDSGI"
BASE COUNT      969 a    788 c    801 g    912 t
ORIGIN      
        1 cgtagtgtat ttaaggcact gcacatgtcg aggaccacga ttattgtcta cagtgcagtc
       61 agtagttgtc aggtgcagca ggcggaccag catttcttta aatctactca atcgatcaag
      121 taagtaactg taatccaagt acagtcggcc catcccactc ggttctgccg taactcaaga
      181 aatttcttga tagcgcagtt ccagttgtaa aacgtcgttt aaaaaaacat ctcgttggag
      241 cttgggttga aaaacgttcg agttgggcgt agaaaagctc aataataaaa tatcaaaaaa
      301 gagaaaatat cttcccaaag cagctgtcca ttttttgtta aagtgataga tcaaaaattc
      361 gtatgacgta tttgccaagt gctctttatg caaataattt cctcaattct aattctacaa
      421 gaagcacttt tggcttttgt ccagaaaaaa caatttgttt acacttgtcg gatgataaca
      481 gaagcggacc gacggctaag taacataaca gtcgcctgtt aagtaatgcc ctcactctct
      541 gttagatggc cgcgcgtgtc gactccgata gaccagcaga cacgcagtag ttttcctcaa
      601 gaacctgaac tctaaacata gacataattt acgatacctt gagactcaaa taaaaataca
      661 agtatgagtg gtattctatt cctgattaga ctcgcacaga cacacgtacg agtattaaca
      721 taaatttgcc ggcaaataac caaaacaaaa cgaacgcaaa cgatccgaac acgtaatgcg
      781 agagataaga aacagaaaag ctcgacgtga aagaagcttc tggcgacagc tcttgccgaa
      841 ctccctataa atacgagaca gaataccaga ataaatataa taacaaactg ttgaattgtc
      901 ccatcgattc ccagatcaaa agacagaatg tctctcacca acaagaacgt cgttttcgtg
      961 gccggtctgg gcggcattgg cctggacacc agccgggagt tggtcaagcg taatctgaag
     1021 gtaagagaga acgaattcca tggaatctat ggaaatccta aatttaaaaa tgtatttttt
     1081 tagaacctgg tcatcctgga tcgcattgac aatccggctg ccattgccga gctgaaggca
     1141 atcaatccca aggtgaccat caccttctat ccctacgatg tgactgtgcc cgtcgctgag
     1201 accaccaagc tcctgaagac catctttgcc caggtgaaga caatcgatgt cctgatcaac
     1261 ggtgctggca tcctggacga tcatcagatc gagcgcacca ttgccgttaa ctacacgggc
     1321 ctggtcaaca ccaccacagc cattctggac ttctgggaca agcgcaaggg cggcccaggc
     1381 ggcatcattt gcaacattgg ctccgtcacc ggtttcaatg ccatctacca ggtgcccgtt
     1441 tactccggct ccaaggcggc ggtggttaac tttacctcct ccctggcggt aagtaatcca
     1501 acgtttttcc tactccccaa aactaaatat ttctttgaaa tcccatagaa actggctccc
     1561 attactggtg tcactgctta cactgtcaat cctggcatca ctaagaccac tctggtccac
     1621 aaattcaact cgtggctgga tgtggagccc cgtgtggcag agaagctgct cgagcatccc
     1681 acccagacct ctcagcagtg cgccgagaac tttgtgaagg ccattgagct gaacaagaac
     1741 ggtgccatct ggaagttgga tctgggcacc ttggagccca tcacatggac ccagcactgg
     1801 gactcgggca tctaaaccag cccagagact ctatgggaca tggcgtttta gcttttagtt
     1861 tctttttttc cactcaattg ttacgtatat acatacacat atggcaataa ggctgatttg
     1921 aaccccgttt tgaatatgat aatacaaaat tatatttgag aaatttcaac aaaatcgata
     1981 agagctatag ctctctttta attaaaaaaa actagataaa aagagcaatg gtcagtggtg
     2041 gtgggcagtg gtgtggtgtg gcctctatcg atttccacac aaaaacttta ctgtttagta
     2101 atagaaaaga acttgagaaa ggcagccaaa agaatgtacg atctgacggg taagcatgtc
     2161 tgctatgtgg ctgactgcgg tggcattgca ctggagacca gcaaggttct catgaccaag
     2221 aatatagcgg tgagtggtag agtggtgtag agagtggaga gtggttctgg ttcttaaaca
     2281 agtctctagt ctctagtctc ttgtctgctg ctggtagctg tcgaaactaa ttaagttaat
     2341 gactttttca tgttttgttt ttgtccgctg acttttgctg ttcaaagcta caattaagtt
     2401 tatgttttga gtttgatcta cttttgctta agggaagtac tttcgattac ggattgctgg
     2461 catataaaaa tagataccaa ggaatgtgca tcatatcata ttgattctat cttgattctt
     2521 tgcctagaaa ctggccattc ttcagagcgt ggaaaacccc ccggccatcg ctcagttaca
     2581 atcgattaag cacagcacac agatcttctt ctggaccttc gatgtgacca tggcccgcga
     2641 ggagatgaag aagtacttcg atgaggtcat ggtccagatg gactacatcg atgtactgat
     2701 caatggggcc accctgtgcg atgagcggaa cattgatgcc accatcaata cgaatctgac
     2761 cggcatgatg aacaccgtgg ccactgtgct gccctatatg gaccgaaaga tgggcggttc
     2821 gggtggactg attgtgaatg tcacctccgt cataggactg gatccatcgc cggtcttttg
     2881 tgcgtacagt gcctcaaagt ttggtgtgat tgggttcacc cgaagtctag cggtgagtgg
     2941 gcgagatctt agcttcaaaa tgctctctaa tgtcttccat tctttcgcag gatcccctgt
     3001 attacaccca aaatggtgtg gctgtcatgg ccgtctgctg tggccccacc aaagtgtttg
     3061 tcgatcggga actgactgcc ttcctggaat acggccagtc ctttgccgat cgcctgcgtc
     3121 gtgccccctg ccaatcgacg gccgtctgcg gccagaatat agtaaatgcc atcgagagat
     3181 cggagaacgg acagatctgg attgccgaca agggcggcct ggagtcggtg gccctccact
     3241 ggtattggca tatggccgat cagtttgtga actatatgca gagcactgac gatgaggatc
     3301 aggagttttt tttgggccag cgatgaggca tgccttgatc cacggaataa aacatcaaat
     3361 gtgtcacaaa aagtaggcaa cttgtttgtg ggtctggaaa tagatttgaa atattttgag
     3421 gtcatatgaa taatgacata aatgagggtg cctgaaagta tgccaagttt
//