From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Homo sapiens skeletal muscle alpha 2 actinin (ACTN20 mRNA, complete
Date: 21 Jul 92 10:05:15 GMT
Distribution: bionet
Checksum: 06875     8

LOCUS       HUMACTN2A    4181 bp ss-mRNA            PRI       21-JUL-1992
DEFINITION  Homo sapiens skeletal muscle alpha 2 actinin (ACTN20 mRNA, complete
            cds.
ACCESSION   M86406
KEYWORDS    skeletal muscle alpha-actinin.
SOURCE      Homo sapiens (library: lambda-gt10 of M.Koenig) fetus skeletal
            muscle cDNA to mRNA.
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 4181)
  AUTHORS   Beggs,A.H., Byers,T.J., Knoll,J.H., Boyce,F.M., Bruns,G.A. and
            Kunkel,L.M.
  TITLE     Cloning and characterization of two human skeletal muscle
            alpha-actinin genes located on chromosomes one and eleven
  JOURNAL   J. Biol. Chem. 267, 9281-9288 (1992)
  STANDARD  full staff_entry
FEATURES             Location/Qualifiers
     5'UTR           1..173
                     /gene="ACTN2"
     CDS             174..2858
                     /function="'skeletal and cardiac muscle specific'"
                     /product="alpha-actinin"
                     /gene="ACTN2"
                     /codon_start=1
                     /translation="MNQIEPGVQYNYVYDEDEYMIQEEEWDRDLLLDPAWEKQQRKTF
                     TAWCNSHLRKAGTQIENIEEDFRNGLKLMLLLEVISGERLPKPDRGKMRFHKIANVNK
                     ALDYIASKGVKLVSIGAEEIVDGNVKMTLGMIWTIILRFAIQDISVEETSAKEGLLLW
                     CQRKTAPYRNVNIQNFHTSWKDGLGLCALIHRHRPDLIDYSKLNKDDPIGNINLAMEI
                     AEKHLDIPKMLDAEDIVNTPKPDERAIMTYVSCFYHAFAGAEQAETAANRICKVLAVN
                     QENERLMEEYERLASELLEWIRRTIPWLENRTPEKTMQAMQKKLEDFRDYRRKHKPPK
                     VQEKCQLEINFNTLQTKLRISNRPAFMPSEGKMVSDIAGAWQRLEQAEKGYEEWLLNE
                     IRRLERLEHLAEKFRQKASTHETWAYGKEQILLQKDYESASLTEVRALLRKHEAFESD
                     LAAHQDRVEQIAAIAQELNELDYHDAVNVNDRCQKICDQWDRLGTLTQKRREALERME
                     KLLETIDQLHLEFAKRAAPFNNWMEGAMEDLQDMFIVHSIEEIQSLITAHEQFKATLP
                     EADGERQSIMAIQNEVEKVIQSYNIRISSSNPYSTVTMDELRTKWDKVKQLVPIRDQS
                     LQEELARQHANERLRRQFAAQANAIGPWIQNKMEEIARSSIQITGALEDQMNQLKQYE
                     HNIINYKNNIDKLEGDHQLIQEALVFDNKHTNYTMEHIRVGWELLLTTIARTINEVET
                     QILTRDAKGITQEQMNEFRASFNHFDRRKNGLMDHEDFRACLISMGYDLGEAEFARIM
                     TLVDPNGQGTVTFQSFIDFMTRETADTDTAEQVIASFRILASDKPYILAEELRRELPP
                     DQAQYCIKRMPAYSGPGSVPGALDYAAFSSALYGESDL"
     3'UTR           2859..>4181
                     /gene="ACTN2"
     polyA_signal    3249..3254
                     /gene="ACTN2"
     polyA_site      3269
                     /gene="ACTN2"
     polyA_signal    3415..3420
                     /gene="ACTN2"
     polyA_site      3431
                     /gene="ACTN2"
BASE COUNT     1139 a    977 c   1102 g    963 t
ORIGIN      Chromosome 1q42-q43.
        1 ggaactccgc ttcgcccgag acccagcgcc caggcgtgtc gcccgagagg agccgcgcga
       61 aggtcacccc gcgcccgccg cccgccgccc gccgcctccg tgggtccgtt tgccagtcag
      121 cccgtgcgtc cgagcccctc gcgccccgcc gcagccccgg ccaaccgagc gccatgaacc
      181 agatagagcc cggcgtgcag tacaactacg tgtacgacga ggatgagtac atgatccagg
      241 aggaggagtg ggaccgcgac ctgctcctgg acccagcctg ggagaagcag cagaggaaga
      301 ccttcactgc ctggtgtaac tcccacctaa ggaaagccgg cacccagatt gagaacatcg
      361 aggaagactt caggaatggc cttaagctca tgctgctttt ggaagtcatc tcaggggaaa
      421 ggctgcccaa acctgaccgg ggaaaaatgc ggttccacaa aattgctaat gtcaacaaag
      481 ctttggatta catagccagc aaaggggtga aactggtgtc catcggcgct gaagaaattg
      541 ttgatggcaa tgtgaaaatg accctgggta tgatctggac catcatcctt cgctttgcta
      601 ttcaggatat ttcggttgaa gaaacatctg ccaaagaagg tctgctgctt tggtgtcaga
      661 ggaaaactgc tccttataga aatgtgaaca ttcagaactt ccatactagc tggaaagatg
      721 gccttggact ctgtgccctc atccaccgac accggcctga cctcattgac tactcaaagc
      781 ttaacaagga tgaccccata ggaaatatta acctggccat ggaaatcgct gagaagcacc
      841 tggatattcc taaaatgttg gatgctgaag acatcgtgaa cacccctaaa cccgatgaaa
      901 gagccatcat gacgtacgtc tcttgcttct accacgcttt tgcgggcgcg gagcaggccg
      961 agacagcggc taacaggata tgtaaggttc ttgctgtgaa tcaagagaat gagaggctga
     1021 tggaagaata tgagaggcta gcgagtgagc ttttggaatg gattcgtcgc acgatcccct
     1081 ggctggagaa ccggactccc gagaagacca tgcaagccat gcagaagaag ctggaggact
     1141 tccgggatta ccgccggaag cacaagccac ccaaggtgca ggagaaatgc cagctggaga
     1201 tcaacttcaa cacgctgcag accaagctgc ggatcagcaa ccgtcctgcc ttcatgccct
     1261 ccgagggcaa gatggtgtcg gatattgctg gtgcctggca gaggctggag caggctgaga
     1321 agggttacga ggagtggttg ctcaatgaga ttcggagact ggagcgcttg gaacacctgg
     1381 ctgagaagtt caggcagaag gcctcaacgc acgagacttg ggcttatggc aaagagcaga
     1441 tcttgctgca gaaggattac gagtcggcgt cgctgacaga ggtgcgggct ctgctgcgga
     1501 agcacgaggc gttcgagagc gacctggcag cgcaccagga ccgcgtggag cagatcgcag
     1561 ccatcgcgca ggagctcaat gaactggact atcacgacgc tgtgaatgtc aatgatcggt
     1621 gccagaaaat ttgtgaccag tgggaccgac tgggaacgct tactcagaag aggagagaag
     1681 ccctagagag aatggagaaa ttgctagaaa ccattgatca gcttcacctg gagtttgcca
     1741 agagggctgc tcctttcaac aattggatgg agggcgctat ggaggatctg caagatatgt
     1801 tcattgtcca cagcattgag gagatccaga gtctgatcac tgcgcatgag cagttcaagg
     1861 ccacgctgcc cgaggcggac ggagagcggc agtccatcat ggccatccag aacgaggtgg
     1921 agaaggtgat tcagagctac aacatcagaa tcagctcaag caacccgtac agcactgtca
     1981 ccatggatga gctccggacc aagtgggaca aggtgaagca actcgtgccc atccgcgatc
     2041 aatccctgca ggaggagctg gctcgccagc atgctaacga gcgtctgagg cgccagtttg
     2101 ctgcccaagc caatgccatt gggccctgga tccagaacaa gatggaggag attgcccgga
     2161 gctccatcca gatcacagga gccctggaag accagatgaa ccagctgaag cagtatgagc
     2221 acaacatcat caactataag aacaacatcg acaagctgga gggagaccat cagctcatcc
     2281 aggaggccct tgtctttgac aacaagcaca cgaactacac gatggagcac attcgtgttg
     2341 gatgggagct gctgctgaca accatcgcca gaaccatcaa tgaggtggag actcagatcc
     2401 tgacgagaga tgcgaagggc atcacccagg agcagatgaa tgagttcaga gcctccttca
     2461 accactttga caggaggaag aatggcctga tggatcatga ggatttcaga gcctgcctga
     2521 tttccatggg ttatgacctg ggtgaagccg aatttgcccg cattatgacc ctggtagatc
     2581 ccaacgggca aggcaccgtc accttccaat ccttcatcga cttcatgact agagagacgg
     2641 ctgacaccga cactgccgag caggtcatcg cctccttccg gatcctggct tctgataagc
     2701 catacatcct ggcggaggag ctgcgtcggg agctgccccc ggatcaggcc cagtactgca
     2761 tcaagaggat gcccgcctac tcgggcccag gcagtgtgcc tggtgcactg gattacgctg
     2821 cgttctcttc cgcactctac ggggagagcg atctgtgatg ctgagcttct gtaatcactc
     2881 atcccatcag aatgcaataa aagcggaagt cacagtttgt ttcctggaaa ctttgacaag
     2941 ctttattaag ttgagagaga gagaggggga aaaaaaaaaa gcctttcgta gttcagtaat
     3001 tgccagcaat ataacacggc taaaatgaag tttttacagt atatgacata gtgcgcttca
     3061 taaataggtt tatttctgag tttttagcaa aatgtaatga aatatcaggt tgatttcttt
     3121 gattaaacag aacaaattac ttgagtaata ggaaattagg aggatctagg gacagaagga
     3181 aagtgaaaaa tgtgaaaata caaaataccc aagatttaag accgggggga aaaaaccaca
     3241 aattggtaaa taaaggtttg ctatttgtaa aaaatttcat ttatctctaa tatgcttatg
     3301 tgattggccc taggggagta tatttgggat tctaatgttt tattttcatg cttatccaaa
     3361 gattactatt gtatcttcaa atgaacttaa tattgtgaga tggaactgcc ggggattaaa
     3421 aagactaccc aaaagatttt tggcacttac aatttttaaa atagtttatg tcatctcttc
     3481 attatttagg gctggatggt caactcagtc agtgattttt tgatgcttct cttatcctcc
     3541 agaatagaga cctaaggaca cgtggaagtc agtttaattg ccagagagaa ggatgcaatc
     3601 actaggtgaa atgaggtttt taggattatt tattgattcc aggttcccat gctttttgtt
     3661 agagcttatt agtacaggtt ctcaagagat gaccacataa aagtgctctg tttataaata
     3721 agcaggtttc tgtagtactg actggttcat cacaaggcaa gtcagaaacc agtatccttc
     3781 tagctctcca gtcaggactt ccttatgcct ctagttttat gaccggttaa ggagaagcca
     3841 gagttagagt aggagaggac taattctcag cagcagtgga ggtgagttct ttcttttgcg
     3901 gaagctttac atatgttttg tgtagtagga ataactagat attttagcta gtgtgcggtg
     3961 tgtgttcacc cctgggattg gacagtgtat cctaacaagt cccatgtctg gttctgtgtc
     4021 taaaggcctg ctccatgaca caggatgcta catgcactcc tgctagcaca tcttgatctg
     4081 ttgaatgttc attctttctt tttgctcata ctgctgtagg ctataattcc cccctgtttt
     4141 tccatcttgt tgacagcttg tagagaataa agcaggaatt c
//