From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: V. parahaemolyticus tryptophan operon including a leader peptide
Date: 28 Dec 92 21:47:02 GMT
Distribution: bionet
Checksum: 39410 17

LOCUS       VIBTRPOP     7419 bp ds-DNA             BCT       02-DEC-1992
DEFINITION  V. parahaemolyticus tryptophan operon including a leader peptide
            gene, anthranilate synthase alpha-subunit and beta-subunit genes,
            and anthranilate phosphoribosyltransferase gene.
ACCESSION   X17149
KEYWORDS    TrpE gene; anthranilate phosphoribosyltransferase;
            anthranilate synthase; anthranilate synthase alpha subunit;
            anthranilate synthase beta subunit; indo-3-glycerol phosphate;
            indoleglycerol phosphate; operon;
            phosphoribosylanthranilate isomerase; trpA gene; trpB gene;
            trpC gene; trpC(F) gene; trpD gene; trpF gene; trpG gene;
            tryptophan synthase; tryptophan synthase alpha subunit;
            tryptophan synthase beta-subunit.
SOURCE      Vibrio parahaemolyticus DNA.
  ORGANISM  Vibrio parahaemolyticus
            Prokaryotae; Facultatively anaerobic gram-negative rods;
            Vibrionaceae.
REFERENCE   1  (bases 1 to 7419)
  AUTHORS   Crawford,I.P., Han,C.Y. and Silverman,M.
  JOURNAL   Unpublished (1989)
  STANDARD  full automatic
REFERENCE   2  (bases 1 to 7419)
  AUTHORS   Crawford,I.P., Han,C.Y. and Silverman,M.
  TITLE     Sequence and features of the tryptophan operon of Vibrio
            parahemolyticus
  JOURNAL   DNA Sequence 1, 189-196 (1991)
  STANDARD  full automatic
COMMENT     In V. paraheomolyticus the trpC and trpF genes are fused together.

               From EMBL 26   entry VPTRPOP;  dated 19-MAR-1991.
FEATURES             Location/Qualifiers
     prim_transcript 190..>7419
     mat_peptide     220..342
                     /function="leader peptide"
                     /codon_start=1
     mat_peptide     473..2095
                     /EC_number="4.1.3.27"
                     /product="anthranilate synthase, alpha subunit"
                     /gene="trpE"
                     /codon_start=1
     mat_peptide     2104..2691
                     /EC_number="4.1.3.27"
                     /product="anthranilate synthase beta subunit"
                     /gene="trpG"
                     /codon_start=1
     mat_peptide     2714..3709
                     /EC_number="2.4.2.18"
                     /product="anthranilate phosphoribosyltransferase"
                     /gene="trpD"
                     /codon_start=1
     mat_peptide     3720..5162
                     /EC_number="4.1.1.48"
                     /product="phosphoribosylanthranilate isomerase (trpF)"
                     /gene="trpC(F)"
                     /codon_start=1
                     /note="trpC and TrpF are fused"
     mat_peptide     5206..6393
                     /EC_number="4.2.1.20"
                     /product="tryptophan synthase; beta subunit"
                     /gene="trpB"
                     /codon_start=1
     mat_peptide     6396..7199
                     /EC_number="4.2.1.20"
                     /product="tryptophan synthase alpha-subunit"
                     /gene="trpA"
                     /codon_start=1
     CDS             220..345
                     /function="leader peptide"
                     /codon_start=1
                     /translation="MLQEFNQNQKAKVAVCLNKTNSADLAWWRTWTSSWWANVYF"
     CDS             473..2098
                     /EC_number="4.1.3.27"
                     /gene="trpE"
                     /product="anthranilate synthase; alpha subunit"
                     /codon_start=1
                     /translation="MRLCTAGRLKQLQGGLVNKAIEIKKLGQLEVLKASVPYTQDPTR
                     LFHTICENKTDSLLLESAEIDSKQNLKSLLIVDSAVRIVCYGHTVSFHALTENGKNLL
                     THLNQNVRGEVASQFDGETLTLEFIQPCDTIDEDSRLREASSFDALRLVQHSFDLSSQ
                     DKHAIFLGGLFAYDLVANFEPLGDAVATNQCPDYVFYVAETLLVVDHQTESCQLQATL
                     FVSGSQKAALESRIADIRAQCTSPKRLPDATQVANITAQPSVSDQDFCQIVRDLKEFV
                     VKGDIFQVVPSRRFTLPCPSPLAAYKELKQSNPSPYMFYMQDELFTLFGASPESALKY
                     ETDTNQIEIYPIAGTRRRGKRPNGEIDFDLDGRIELELRSDKKENAEHMMLVDLARND
                     VARISQAGTRHVADLLKVDRYSHVMHLVSRVVGQLRDDLDALHAYQACMNMGTLTGAP
                     KIRAMQLIRDVEGARRGSYGGAVGYLTGEGTLDTCIVIRLAYVENGIAQVQAGAGVVF
                     DSDPQAEADETRGKAQAVISAIQAAHSQPANKE"
     CDS             2104..2694
                     /EC_number="4.1.3.27"
                     /gene="trpG"
                     /product="anthranilate synthase; beta subunit"
                     /codon_start=1
                     /translation="MANIVFIDNFDSFTYNLVDQFRSLGHSVKIYRNHIPAETIEQAI
                     NELENPVVLLSPGPGAPSEAGSMPELIQRMKGKVPMIGICLGHQAIVEAYGGTVAGAG
                     EIIHGKVSMMEHQDHAIYQNLPSPLAIARYHSLVATKVPDSLTITAEVDNLVMSVVHE
                     QDKVCGFQFHPESIMTTYGATLLGNAIEWALEKNNA"
     CDS             2714..3712
                     /EC_number="2.4.2.18"
                     /gene="trpD"
                     /product="anthranilate phosphoribosyltransferase"
                     /codon_start=1
                     /translation="MEAIINKLYEQQSLTQEESQQLFDIIIRGELDPILMASALTALK
                     IKGETPDEIAGAAKALLANANPFPRPDYDFADIVGTGGDGHNTINISTTAAFVAAACG
                     LKVAKHGNRSVSSKSGSSDLLDSFGINLAMSAEDTRKAVDDIGVAFLFAPQYHGGVRH
                     AMPVRQTMKTRTIFNILGPLINPARPNIELMGVYSEELVRPIAETMLQMGMKRAAVVH
                     GSGLDEVAIHGTTTVAEIKDGKITEYTLTPEDFGLESHPLEAIKGGDPEENKAIITNI
                     LTGKGTDAQLGAVAVNVALLMRLFGHEDLKANTQQAIEAMNSGKAYQLVQQLAAHA"
     CDS             3720..5165
                     /EC_number="4.1.1.48"
                     /gene="trpC(F)"
                     /note="trpC and TrpF are fused"
                     /product="phosphoribosylanthranilate isomerase (trpF)"
                     /codon_start=1
                     /translation="MKMTDFNTQQANNLSEHVSKKEAEMAEVLAKIVRDKYQWVAERK
                     ASQHLSTFQSDLLPSDRSFYDALSGDKTVFITECKKASPSKGLIRNDFDLDYIASVYN
                     NYADAISVLTDEKYFQGSFDFLPQVRRQVKQPVLCKDFMVDTYQVYLARHYGADAVLL
                     MLSVLNDEEYKALEEAAHSLNMGILTEVSNEEELHRAVQLGAKVIGINNRNLRDLTTD
                     LNRTKALAPTIRKLAPNATVISESGIYTHQQVRDLAEYADGFLIGSSLMAEDNLELAV
                     RKVTLGENKVCGLTHPDDAAKAYQAGAVFGGLIFVEKSKRAVDFESARLTMSGAPLNY
                     VGVFQNHDVDYVASIVTSLGLKAVQLHGLEDQEYVNQLKTELPVGVEIWKAYGVADTK
                     PSLLADNIDRHLLDAQVGTQTGGTGHVFDWSLIGDPSQIMLAGGLSPENAQQAAKLAC
                     LGLDLNSGVESAPGKKDSQKLQAAFRAIRNY"
     CDS             5206..6396
                     /EC_number="4.2.1.20"
                     /gene="trpB"
                     /product="tryptophan synthase; beta subunit"
                     /codon_start=1
                     /translation="MAKLNAYFGEYGGQYVPQILVPALEQLEQAFIDAQEDPEFRSEF
                     MTLLQEYAGRPTALTLTRNLTKGTKTKLYLKREDLLHGGAHKTNQVLGQALLAKRMGK
                     HEIIAETGAGQHGVATALACALLGLKCRVYMGAKDVERQSPNVFRMKLMGAEVIPVHS
                     GSATLKDACNEALRDWSGSYEDAHYLLGTAAGPHPFPTIVREFQRMIGEETKNQILAR
                     EGRLPDAVIACVGGGSNAIGMFADFIEEESVRLIGVEPAGKGIDTDQHGAPLKHGKTG
                     IFFGMKAPLMQDENGQVEESYSVSAGLDFPSVGPQHAHLNAIGRAEYDNVTDDEALEA
                     FQELARSEGIIPALESSHALAHALRMARENPEKEQLLVVNLSGRGDKDIFTVHAILEE
                     KGVI"
     CDS             6396..7202
                     /EC_number="4.2.1.20"
                     /gene="trpA"
                     /product="tryptophan synthase alpha-subunit"
                     /codon_start=1
                     /translation="MSRYEKMFARLNEKNQGAFVPFVTVCDPNAEQSYKIMETLVESG
                     ADALELGIPFSDPLADGPTIQGANIRALDSGATPDICFEQIGKIRAKYPDLPIGLLMY
                     ANLVYSRGIESFYERCAKAGIDSVLIADVPTNESAEFVAAAEKFGIHPIFIAPPTASD
                     ETLKQVSELGGGYTYLLSRAGVTGAETKANMPVDHMLEKLNQFDAPPALLGFGISEPA
                     QVKQAIEAGAAGAISGSAVVKIIEAHVEQPQIMLDKLGELVSAMKAATQK"
BASE COUNT     2139 a   1767 c   1729 g   1784 t
ORIGIN      
        1 caaccgtgtc gtgatctgta atcgccaaaa cctcgatatc aaaacccagt gccctatcca
       61 ctaaatcttt cggttctaat cgaccatcgg acgccgtggt gtgactatgt aaatcaattc
      121 tcatattttt gtcgttttaa tgctgttttg gggttgactt tttactctcg cactagttaa
      181 ctagtacaca aatacaaagc acgtaattta aggctcacta tgttacaaga atttaaccag
      241 aaccaaaaag cgaaagttgc tgtttgtctt aataagacaa actcagcaga tcttgcttgg
      301 tggcgcactt ggacaagttc ttggtgggct aacgtgtact tctagtttag aaaaacaaac
      361 gtcataaagc ccgctactca agcgggcttt ttattttgtc gcccaactca cattcaactg
      421 cctaaacatt aatcggatat gaccaactct tgattgggag aacgtttgct ttatgcgact
      481 atgtactgca ggacgattaa aacagctaca aggaggtctt gtgaacaagg ccattgaaat
      541 taagaaactt ggacaactgg aagtattaaa agcttctgtt ccttacactc aagacccaac
      601 ccgtctgttc catactattt gtgagaacaa aacggatagc ttgcttttag aatcggctga
      661 aattgattct aaacagaatc tcaaatcgct gttaatcgtc gattctgcgg ttcgcatcgt
      721 ttgttatgga cataccgtca gctttcatgc tctaacggag aacggcaaaa acctactgac
      781 gcatttgaat caaaacgtac gtggcgaagt cgcaagccaa tttgacggcg aaacactgac
      841 tttagaattc atccaaccat gcgatacgat tgatgaagat tctcgtctac gagaagcctc
      901 ttcttttgat gcgcttcgtt tggtacagca cagttttgat ctttcttcgc aagacaaaca
      961 tgccattttc cttggtggcc tatttgcgta tgacttggtg gcgaacttcg aaccattagg
     1021 agacgcggta gcaacaaacc agtgtccgga ttacgtcttc tatgttgcgg aaacattact
     1081 ggttgtagat caccaaacag aatcctgcca actgcaagca acattgttcg ttagcggctc
     1141 gcaaaaagca gcgctagaaa gtcgaatcgc agatatccga gctcaatgca catcgccaaa
     1201 gcgtcttcca gacgcaacgc aagtggccaa tatcacagct caaccaagcg tttcggatca
     1261 agacttctgt caaatcgtcc gtgatctaaa agaattcgtg gtaaaaggcg atattttcca
     1321 agtagttcct tctcgtcgat tcacattgcc ttgcccttct ccgctagcag cttacaaaga
     1381 gctaaagcaa agcaacccaa gcccttacat gttctacatg caagatgagc tgttcacttt
     1441 gtttggtgcg tctccagaaa gtgccttgaa gtatgaaacc gacaccaacc aaatagaaat
     1501 ctacccaatt gcggggactc gccgtcgcgg taaacgtcca aatggcgaaa tcgattttga
     1561 tttagatggc cgtattgaac ttgaactacg tagcgacaaa aaagagaacg ctgaacatat
     1621 gatgttggta gacctagctc gtaatgatgt agcgcgcatc tcgcaagctg gtactcgtca
     1681 tgttgccgat ctccttaaag tcgaccgcta tagccatgtt atgcacttgg tgtcccgcgt
     1741 ggttggccag ttgcgcgatg accttgatgc tttgcacgct taccaagctt gtatgaacat
     1801 gggcacgtta actggagcgc caaaaattcg tgcaatgcaa ctgattcgcg atgtcgaagg
     1861 tgctcgtcgt ggcagctatg gcggagcggt gggctacctg acaggagaag gcacgctcga
     1921 cacttgtatc gtgattcgct tggcttacgt ggaaaacggc attgctcaag tacaagcagg
     1981 tgcaggcgtg gtattcgatt ctgaccctca agcggaagcc gatgaaacac gcggaaaagc
     2041 tcaagcggtt atctctgcta ttcaagcagc gcattctcaa cctgcgaaca aggagtagca
     2101 gacatggcga acatcgtatt catcgataac tttgactcgt tcacctataa ccttgtggac
     2161 caattccgct cattaggtca ctcagtcaaa atttatcgta accacattcc ggcagaaacc
     2221 atcgaacaag caataaacga gctcgaaaac cccgtcgttc tgctatcacc ggggccaggt
     2281 gcgccatcag aagctggttc gatgccagag ctgattcaac gaatgaaagg caaagtgcca
     2341 atgattggta tttgtcttgg tcaccaagcg attgttgaag cgtacggcgg tactgtcgct
     2401 ggcgctggtg aaatcattca cggcaaagtg tcgatgatgg agcaccaaga ccacgcaatt
     2461 tatcaaaatc tgccatcgcc acttgcaatt gcgcgttacc attctttagt ggcgacaaag
     2521 gtaccagaca gcctgaccat cactgccgaa gtagacaatt tggtcatgtc tgttgtgcac
     2581 gaacaagata aagtttgcgg atttcagttc cacccagagt caataatgac gacttacggc
     2641 gcaaccttgt tgggcaacgc gattgaatgg gcgcttgaga aaaataacgc ataaggaaat
     2701 ggggagagaa actatggaag caatcatcaa caaactgtac gaacagcaat cactgactca
     2761 agaagaaagc caacagctat tcgacattat tattcgcggc gagcttgatc caattttaat
     2821 ggcatcggca ttaacagcat taaagatcaa aggcgaaacg cctgatgaaa ttgccggtgc
     2881 agcaaaagcc ctactggcga atgcgaaccc gttccctcgt cctgattacg actttgcaga
     2941 catcgtaggg acagggggcg atggtcacaa tactatcaat atctcgacga cggccgcctt
     3001 tgttgcagca gcatgtggct taaaagtcgc gaaacacggc aaccgcagcg tatcaagtaa
     3061 atccggctct tctgacttac tggactcgtt tggcatcaac ctagcaatga gtgctgaaga
     3121 cacccgcaaa gcggttgacg acattggcgt tgccttcttg ttcgcgcctc aatatcacgg
     3181 aggcgttcgc cacgcgatgc ctgtgcgcca aactatgaaa acgcgcacca ttttcaacat
     3241 tcttggcccg ttgataaatc ctgcacgtcc aaacatcgaa ctaatgggcg tctacagtga
     3301 agaactggtt cgtcctatcg cagaaaccat gctacaaatg ggtatgaagc gcgccgctgt
     3361 cgtacatggt agcggattgg atgaagtggc cattcacggt acaacaacgg ttgccgaaat
     3421 caaagatggc aagatcactg aatacaccct aacgccagaa gattttggtc tagagtctca
     3481 cccgctagaa gccattaaag gcggcgaccc ggaagaaaac aaagcaataa tcaccaacat
     3541 tctgacgggt aaaggtacag acgcacaatt aggcgcagtc gcggttaacg tcgcacttct
     3601 aatgcgttta ttcggccacg aagatttaaa agccaacacg cagcaagcga tcgaggctat
     3661 gaactcaggc aaagcttacc aattggtaca gcaacttgcc gcgcacgctt aaggaacaga
     3721 tgaagatgac tgacttcaac acccagcaag caaacaactt atcagagcac gtctcaaaaa
     3781 aagaagcaga aatggcggaa gtgcttgcca aaattgttcg cgataaatac caatgggtag
     3841 cagagcgaaa agcatcacag catctgagca cttttcaatc ggatctattg ccgtcggatc
     3901 gcagttttta tgatgcattg agtggcgata aaaccgtatt cattacagaa tgcaaaaagg
     3961 catcgccatc aaaaggcctt attcgcaacg acttcgattt ggactacatc gcgtcggttt
     4021 acaacaacta cgcagacgcg atctcggttc tgaccgatga aaaatacttc cagggtagct
     4081 tcgattttct acctcaagtt cgccgacaag tgaaacaacc agtgttgtgt aaggacttca
     4141 tggtcgatac ctaccaagtt taccttgctc gtcattatgg cgcggatgcg gtattgctca
     4201 tgctttcggt actgaatgac gaagagtaca aagcgttaga agaagctgcg cacagcctaa
     4261 atatgggcat tttaacggaa gtcagcaacg aagaagagct tcaccgcgca gttcagcttg
     4321 gcgctaaagt tatcggtata aacaaccgca acttgcgcga cttaacaacg gatttaaacc
     4381 gaactaaagc gcttgcgcca accattcgta agcttgcacc aaacgcaacg gttatttcag
     4441 agtctggcat ttacactcat caacaagttc gtgacttagc agaatatgca gacggctttt
     4501 tgattggcag ctcgctgatg gcggaagaca acttagaact tgcggttcgt aaagtaacgc
     4561 taggtgaaaa taaggtttgc ggtctgactc acccagatga tgcggcaaaa gcgtatcaag
     4621 caggcgcagt atttggtggt ctgattttcg ttgagaaatc gaaacgagcg gtcgattttg
     4681 aaagtgctcg cctaaccatg agcggcgcgc cacttaatta tgttggcgtg ttccaaaatc
     4741 acgacgttga ttatgtcgcc tctatcgtaa cgtctttggg cttaaaagcc gtacagttac
     4801 atgggctaga agatcaagag tacgtcaacc agctcaaaac agaactaccc gttggcgtcg
     4861 agatttggaa agcctacggc gtggcggata caaagccatc cttgctcgca gacaacattg
     4921 accgtcacct actggatgct caagttggta ctcaaactgg cggcacaggc cacgtgttcg
     4981 actggtcttt gatcggcgat ccaagtcaaa tcatgctggc aggtggactg tcgcctgaaa
     5041 atgcacagca agcagcaaaa ttagcctgct tgggattaga tttaaactct ggagtagaaa
     5101 gcgcaccggg gaaaaaagat tcacagaagt tgcaagccgc atttcgcgct attcgtaact
     5161 actaaagtta ttcgcagtta taaaaatacg agaaggatta tcaaaatggc aaaactgaat
     5221 gcctactttg gcgaatatgg cggtcaatac gttccgcaaa ttctagttcc agcacttgag
     5281 caactagagc aagcgtttat tgacgcgcaa gaagatccag aattccgcag cgagttcatg
     5341 acgctccttc aggagtacgc tggtcgccca acggcactga ctctgactcg taacctaacc
     5401 aaaggcacaa aaacaaaatt gtacctaaaa cgtgaagatc tgcttcacgg cggcgcacac
     5461 aaaaccaacc aagtacttgg ccaggcgctg cttgcgaagc gcatgggtaa acacgaaatc
     5521 atcgcagaaa caggcgcagg tcaacacggt gtagcaactg cacttgcgtg tgcactactt
     5581 ggccttaagt gccgagttta catgggtgca aaagacgttg aacgtcaaag cccgaacgtg
     5641 ttccgcatga aactgatggg tgcagaagta attcctgtcc attcaggctc agcaacactt
     5701 aaagatgctt gtaatgaagc gctgcgtgat tggtcaggca gctacgaaga cgcacactac
     5761 cttctcggta cagcagcagg ccctcaccca ttcccgacta ttgtgcgtga gttccaacgt
     5821 atgattggtg aagaaactaa aaaccaaatt ctggcgcgtg aaggtcgcct acctgatgct
     5881 gttatcgcct gtgtgggtgg tggctcaaac gcgattggta tgtttgcaga ttttatcgaa
     5941 gaagaaagcg ttcgcctgat tggcgtggag ccagcaggta aaggcatcga tacagatcaa
     6001 cacggtgcgc cacttaagca tggtaaaaca ggtatcttct tcggtatgaa ggcaccatta
     6061 atgcaagatg agaatggtca ggttgaagag tcttactctg tttcagcagg tctagacttc
     6121 ccttctgttg gtccacagca cgcacacctt aacgctatcg gtcgcgctga atacgacaac
     6181 gtaacggatg atgaagcatt agaagcattc caagaactag ctcgcagcga aggcatcatt
     6241 cctgcacttg aatcctccca tgctctggca catgcactgc gcatggctcg tgagaatcct
     6301 gaaaaagagc aattactggt tgttaaccta tctggtcgag gtgacaaaga catcttcacc
     6361 gtacacgcta tcttagaaga aaaaggagta atctaatgag ccgttatgag aagatgtttg
     6421 cacgcctaaa cgagaaaaac caaggcgcat ttgtaccatt cgtgacggtt tgtgatccaa
     6481 acgccgagca atcgtacaag atcatggaaa cactggttga atctggcgct gatgcgcttg
     6541 aacttggtat tccgttctca gacccattgg cggacggccc aacaattcaa ggtgccaaca
     6601 ttcgagcgtt agattcgggt gcaactccgg atatctgttt tgagcaaatc ggaaaaattc
     6661 gagcaaagta cccagacctg ccaattggtc tattgatgta cgccaatctt gtatactcac
     6721 gcggtataga gagcttctac gagcgctgtg caaaagcggg catcgattcc gttctcattg
     6781 cagacgttcc gactaacgaa agcgcagagt tcgtagccgc ggcagagaaa tttggaatcc
     6841 acccaatctt tatcgcaccc ccaacggcaa gtgacgagac gctaaaacaa gtttctgaat
     6901 taggcggcgg ctacacttac ctgctttcgc gggcgggtgt gacgggtgca gaaaccaaag
     6961 ctaacatgcc agttgaccac atgcttgaaa agctaaacca gtttgatgca ccaccagcgt
     7021 tacttggttt cggcatttct gaaccagcac aagtaaaaca agccattgaa gcgggtgctg
     7081 caggtgcaat ttcgggttca gcagtcgtaa aaatcatcga agcacacgta gagcaaccgc
     7141 aaatcatgct cgataagctt ggtgaacttg tctcagcaat gaaagcggca actcaaaagt
     7201 aatcgacacc acaagctaaa cactgcaaac gctcattgat tctcgttaat gggcgttttt
     7261 ttatcaactt gattgcaaac ctcgcaatgc tctcacttcc aaccgtcttt taaagaagcg
     7321 aatccataaa gtgataacat gccattgttc aaacctgttt tgtaaggtac tgacatggaa
     7381 gatatcaaat cgttgttgct gcaatgccct gttttagcc
//