From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Thermoanaerobacterium saccharolyticum sp. amylopullulanase gene,
Date: 29 Dec 92 21:28:41 GMT
Distribution: bionet
Checksum: 40540 9

LOCUS       TEOAPULNS    4375 bp ds-DNA             BCT       14-DEC-1992
DEFINITION  Thermoanaerobacterium saccharolyticum sp. amylopullulanase gene,
            complete cds.
ACCESSION   L07762
KEYWORDS    amylopullulanase.
SOURCE      Thermoanaerobacter saccharolyticum (strain B6A-RI) DNA.
  ORGANISM  Thermoanaerobacter saccharolyticum
            Prokaryotae; Irregular, nonsporing, gram-positive rods.
REFERENCE   1  (bases 1 to 4375)
  AUTHORS   Matur,R.V. and Zeikus,J.G.
  TITLE     Molecular cloning, expression in E. coli and sequencing of
            amylopullulanase gene of Thermoanaerobacterium saccharolyticum sp.
            nov
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     RBS             317..323
     sig_peptide     331..435
                     /codon_start=1
     CDS             331..4197
                     /evidence="EXPERIMENTAL"
                     /product="amylopullulanase"
                     /codon_start=1
                     /translation="MYKKLFTKKFISFVMSLLLVLTAAFSSMPFHNVYAADNASVVAN
                     IVGDFQDQLGDSNWNIDSNITIMQYVGNGLYEFTTPTQLKAGSYQYKVALNHSWNGGG
                     VPSQGNLTLNLTNDSYVTFWFDYNTQSVTDSTKYTPISNDKLPRLVGTIQSAIGAGKD
                     WDPGTSTAIMIDDNFDNVYSYTAHIPKGDYQYKVTLGNTWAENYGANGVQDGSNIQLS
                     VANDADITFFYDANTHNIWTNYSPTLTGLDNNIYYDDLKHDTHDPFFRNPFGAIKVGQ
                     TVTLRIQAKNHDLESARISYWDDINKTRTELPMTRIGESPDGNYEYWEIKLSFDHPTR
                     IWYYFILKDGTKTAYYGDNDDQLGGLGKATDTVNKDFELTVYDKNFDTNDWMKGAVMY
                     QIFPDRFYNGDTSNDHAKTLSRGNDPIEFHNDWNDLPDNPNNAGTPGYTGDGIWSNDF
                     FGGDLKGIDDKLDYLKGLGVSVIYLNPIFESPSNHKYDTADYTKIDEMFGTTQDFEKL
                     MSDAHAKGINIILDGVFNHTSDDSIYFNRYGKYPDLGAYQDWKDGNQSLSPYGDWYTI
                     NSDGTYECWWGYDSLPVIKSLNGSEYNVTSWANFIINDKNAISKYWLNPDENLNDGAD
                     GWRLDVENEVAHDFWTHFRDAINTVKPEAPMIAENWGDASLDLLGDSFNSVMNYQFRN
                     DIIDFLIGQSFDDGNGQHNPIDAAKLDQRLMSIYERYPLPAFYSTMNLLGSHDTMSIL
                     TVFGYNSADPNENSDAAKRLAEQKLKLATILQMGYPGMADIYYGDEAGVSGGKDPDDR
                     RTFPWGNEDTALQDFFKNVSSIRNNNQVLKTGDLETLYAQNDVYAIGRRIINGKDAFG
                     NSYPDSAAIVAINRSNSDQQITIDTTKFLRDGVAFKDLINGDKSYTINGGQITINIPA
                     MSGVMLISDDGQDLTAPQVPSNVVATSGNGKVDLSWSQSDGATGYNIYRSSVEGGLYE
                     KIASNVTGTTFEDTNVTNGLKYVYAISAVDELGNESEMSIDTVAYPAYPIGWVGNLTQ
                     VVDNHVISVSNPTEDIYAEVWADGLTNSTGQGPNMIAQLGYKYVGGTVNDSVYGSVYN
                     SVYGVDDSDFTWVNAQYVGDIGNNDQYKASLHLINRSMGYLMRFSDNQGQSWTTTDTL
                     SFYVVPSDDLIKPTAPILNQPGVESSRVSLTWSPSTDNVGIYNYEIYRSDGGTFNKIA
                     TVSNEVYNYVDTSVINGTTYSYKVVAADPSFNRTESNVVTIKPDVVPIKVTFNVTVPD
                     YTPNSVNLAGTFPNATWDPRVPSSNS"
BASE COUNT     1505 a    666 c    866 g   1338 t
ORIGIN      
        1 attcattcat agtcattttt ttataataag tagaaatttt agtaatttat ttgttgaaag
       61 caatattgta gagctttcaa accattatta aaatttctcc tatattaaaa caatagacat
      121 tattgacatt cttcggcaat aattatataa tattattcaa gaagaaatcg gttgcgcaac
      181 ttttgcacat tcagctcatt acaataatct caaattattt tattttaaat taatttcagc
      241 agataaatga ctttaaaagg aggtgaaaac ataggacata aattaatata aatttatttc
      301 aatccaaaaa attcacgggg aggcattaac gtgtacaaaa aactttttac taagaaattt
      361 ataagttttg ttatgtcgct tttgttggta ttgacagcgg cattttcttc aatgccattt
      421 cacaatgtat atgctgctga caatgcaagc gttgtagcaa atatcgttgg ggactttcaa
      481 gaccaactgg gtgattctaa ctggaatatt gatagtaaca ttaccataat gcaatatgta
      541 ggaaatggac tgtatgaatt tactacgcct actcaactaa aagctggtag ttatcagtat
      601 aaagttgcat taaatcattc atggaatggt ggaggtgttc catctcaagg aaatttaacg
      661 ctgaacctaa caaatgattc atatgttaca ttctggtttg actataatac acaatctgta
      721 actgattcta caaagtatac tccaatatct aatgacaaat tacctagatt agttggaaca
      781 attcaatcag ctattggagc aggtaaagat tgggaccccg gaacatcaac agcaataatg
      841 atagatgata attttgacaa cgtttattca tatactgctc atataccaaa gggtgactat
      901 cagtataagg tgacattagg gaatacatgg gccgaaaact atggtgcaaa tggtgtacaa
      961 gatggttcta acatacagct aagtgtcgca aatgatgctg atataacgtt tttttatgat
     1021 gcaaataccc acaatatctg gactaattat agtccaacac ttactggttt agataacaac
     1081 atatattatg atgatctaaa gcatgataca catgatccat tttttaggaa tccttttgga
     1141 gctataaaag tgggacaaac cgttacatta agaattcaag ccaaaaatca tgatctcgaa
     1201 tctgccagaa tatcgtattg ggatgatata aataagacga ggacggaatt acctatgaca
     1261 agaataggtg aaagtcctga tggtaattat gaatattggg agattaagtt aagctttgat
     1321 catccaacga gaatttggta ttactttata ttaaaagatg gaactaaaac tgcttattat
     1381 ggtgacaatg atgaccaact tggtgggtta gggaaggcaa cagatactgt aaataaagac
     1441 tttgaattaa ctgtatacga taaaaatttt gatacgaatg attggatgaa aggcgctgtt
     1501 atgtatcaaa ttttccctga caggttttat aatggagata catccaatga tcatgcaaag
     1561 acgttaagtc gaggcaatga cccaatagag ttccataatg attggaatga cctcccggat
     1621 aatcccaata atgctggaac accaggttat actggtgatg gtatttggtc taatgacttt
     1681 tttggtggtg atttaaaagg aatcgatgat aaacttgatt atctgaaagg gcttggagta
     1741 tccgtaattt acttaaatcc aatctttgaa tcgccttcaa atcataaata tgatacagct
     1801 gactatacaa agattgatga aatgtttggt actacccagg actttgaaaa gttaatgagt
     1861 gatgcacatg ctaaagggat aaatataatt cttgatggtg tattcaatca caccagtgat
     1921 gatagtatct attttaatag atacggcaag tatccagatt tgggtgcata tcaagattgg
     1981 aaagatggaa atcaatcttt atcaccgtat ggtgattggt ataccattaa ttcagacggt
     2041 acttacgagt gttggtgggg ctatgatagc ttacctgtta taaagtcatt aaatggcagt
     2101 gagtataacg ttacaagttg ggctaacttc ataataaacg ataaaaatgc gatttcgaag
     2161 tattggctaa atccagacga aaatttaaat gatggcgctg atggttggcg tttagatgtt
     2221 gaaaatgaag ttgcacatga tttttggaca cattttaggg atgccattaa tactgtaaaa
     2281 cctgaagctc caatgatagc agaaaattgg ggagatgcat ctctcgattt acttggcgat
     2341 tcttttaatt cagttatgaa ttaccagttt agaaatgaca taatagattt cttgattgga
     2401 caatctttcg atgatggaaa tggacaacac aatcctatag atgcagcaaa acttgaccaa
     2461 agattaatga gcatatatga aagatatcca ttgcctgctt tttattcaac aatgaactta
     2521 cttgggtcgc atgatacaat gagtatatta acggtatttg gatataactc tgcagatcct
     2581 aacgaaaact cagacgctgc taaacgactg gcagaacaaa agcttaagtt agcaacaatt
     2641 ttgcagatgg gctatcctgg gatggcagat atttactatg gcgatgaagc aggagtttct
     2701 ggcggtaaag atccagatga tagaagaaca ttcccttggg gaaatgaaga cactgcatta
     2761 caagatttct tcaaaaatgt atcaagcatt agaaataaca atcaagtatt aaaaactggt
     2821 gatttagaga ctttatatgc acaaaatgat gtttatgcca ttggtaggag aattataaat
     2881 ggcaaagatg cttttggtaa ttcgtatcct gatagcgctg caattgttgc aataaatagg
     2941 agcaattcag atcaacaaat taccattgat acgacaaagt ttttaagaga tggagttgcg
     3001 tttaaagatt taatcaacgg agataaatct tatacaataa atggtggaca gataactata
     3061 aatatacctg ccatgagtgg tgtaatgctc atatcagatg atggacaaga tttaactgct
     3121 ccacaagttc cttcaaatgt agttgcgact tccggcaatg gtaaagttga tttatcgtgg
     3181 tcgcaatctg atggtgcaac aggttacaat atatatcgtt catcagtaga aggtggttta
     3241 tatgaaaaaa tagcatcaaa tgtaactgga acaacgtttg aggataccaa tgttacaaat
     3301 ggattaaaat atgtatacgc aatatcagca gttgatgaat taggcaatga aagcgaaatg
     3361 agtattgata cagtagcata tccagcgtat cctataggat gggtaggaaa tcttacacaa
     3421 gtagttgata accatgtaat aagtgttagt aatccaacag aagatattta tgcagaagtt
     3481 tgggcagatg gacttacaaa tagcactggt caaggtccaa atatgatagc gcaattaggg
     3541 tataaatatg ttggtggtac tgtaaatgat tcagtatatg gcagtgttta taatagcgta
     3601 tatggcgtag atgatagtga ctttacctgg gtaaatgctc aatatgttgg agatatagga
     3661 aataatgatc agtacaaagc aagtttacac ctgataaata ggtcaatggg atatttaatg
     3721 agattttcag ataatcaagg tcaaagctgg actacgacag atacattatc gttttacgtt
     3781 gttccttcgg atgacttaat aaagccaact gcacctattt tgaatcagcc aggtgtggaa
     3841 tcatctaggg tctcgcttac atggagtccg tcgacagata acgttggaat atataattat
     3901 gaaatttata gatctgatgg tggaacattt aataaaatag ctactgtatc caatgaggtc
     3961 tataattatg ttgatacaag tgtaatcaat ggaacaacct acagttataa agttgttgct
     4021 gccgatccat catttaacag aacagaatct aatgtagtga ctataaaacc agatgtagtt
     4081 cctataaagg ttacttttaa tgttactgtg ccagattata caccaaattc tgttaatttg
     4141 gctggtacat tccctaatgc aacctgggat ccccgggtac cgagctcgaa ttcgtaatca
     4201 tggtcatagc tgtttcctgt gtgaaattgt tatcgctcac aattccacac aacatacgag
     4261 ccggaagcat aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     4321 gttcctcact gcccgctttc cagtcgggaa acctgtgtgc cactgcatta atgaa
//