From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: D.pseudoobscura Adh gene for alcohol dehydrogenase
Date: 28 Dec 92 21:28:09 GMT
Distribution: bionet
Checksum: 15316 8

LOCUS       DROADHGN79   3469 bp ds-DNA             INV       01-DEC-1992
DEFINITION  D.pseudoobscura Adh gene for alcohol dehydrogenase
ACCESSION   X64488
KEYWORDS    ADH gene; alcohol dehydrogenase.
SOURCE      Drosophila pseudoobscura DNA.
  ORGANISM  Drosophila pseudoobscura
            Eukaryota; Animalia; Metazoa; Arthropoda; Uniramia; Insecta;
            Pterygota; Neoptera; Holometabola; Diptera; Brachycera;
            Cyclorrhapha; Schizophora; Drosophiloidea; Drosophilidae.
REFERENCE   1  (bases 1 to 3469)
  AUTHORS   Schaeffer,S.W. and Miller,E.L.
  TITLE     Estimates of gene flow in Drosophila pseudoobscura determined from
            nucleotide sequence analysis of the alcohol dehydrogenase region
  JOURNAL   Genetics 132, 471-480 (1992)
  STANDARD  full automatic
COMMENT     *source: strain=PS289; *source: isolate=Kiabab National Forest,
            Arizona; *source: chromosome=IV section 88;
               From EMBL    entry DPADHGN79;  dated 16-OCT-1992.
FEATURES             Location/Qualifiers
     mRNA            join(40..119,923..1028,1092..1496,1559..1974)
                     /note="adult mRNA (Adh)"
     mRNA            join(890..1028,1092..1496,1559..1974)
                     /note="larval mRNA (Adh)"
     promoter        8..14
                     /note="adult promoter (Adh)"
     prim_transcript 40..1974
                     /note="adult primary transcript (Adh)"
     exon            40..119
                     /note="adult exon 1a"
     intron          120..922
                     /note="adult intron"
     prim_transcript 890..1974
                     /note="larval primary transcript (Adh)"
     exon            890..1028
                     /note="larval exon 1"
     exon            923..1028
                     /note="adult exon 1b"
     intron          1029..1091
                     /number=1
     exon            1092..1496
                     /number=2
     intron          1497..1558
                     /number=2
     exon            1559..1974
                     /number=3
     polyA_signal    1951..1956
     polyA_site      1974
     CDS             join(936..1028,1092..1496,1559..1825)
                     /EC_number="1.1.1.1"
                     /gene="Adh"
                     /product="alcoholdehydrogenase"
                     /codon_start=1
                     /translation="MSLTNKNVVFVAGLGGIGLDTSRELVKRNLKNLVILDRIDNPAA
                     IAELKAINPKVTITFYPYDVTVPVAETTKLLKTIFAQVKTIDVLINGAGILDDHQIER
                     TIAVNYTGLVNTTTAILDFWDKRKGGPGGIICNIGSVTGFNAIYQVPVYSGSKAAVVN
                     FTSSLAKLAPITGVTAYTVNPGITKTTLVHKFNSWLDVEPRVAEKLLEHPTQTSQQCA
                     ENFVKAIELNKNGAIWKLDLGTLEPITWTQHWDSGI"
BASE COUNT      975 a    784 c    799 g    911 t
ORIGIN      
        1 cgcagtgtat ttaaggcact gcacatgtcg aggaccacga ttattgtcta cagtgcagtc
       61 agtagttgtc aggtgcagca ggcggaccag cattgcttta aatctactca atcgatcaag
      121 taagtaactg taatccaagt acagtcggcc catcccactc ggtcagtcgg ttctgcagta
      181 attcaagaaa tttcttgata gcgcagttcc agttgtaaaa cgtcgtttaa aaaaacatct
      241 cgttggagct tgggttgaaa aacgttcgag ttgggcgtag aaaatttcaa ataaaaaata
      301 tcaaaaaaga gaaaatatct tcccaaagca gctgtccatt ttttgttaaa gtgatagatc
      361 aaaaattcgt atgacgtatt tcccaagtgc tctttatgca aataatttcc tcaattctaa
      421 ttctacaaga agcacttttg gcacttgtcc agaaaaaaca atttgtttac acttttcggt
      481 tgataacaga ggcggaccga cggctaagta acataacagt cgcctgttaa gtaatgccct
      541 cactctctgt tagatggccg cgcgtgtcga ctccgataga ccagcagaca cgcagtagtt
      601 ttcctcaaga acctgaactc taaacataga cataatttac gataccttga gactcaaata
      661 aaaatacaag tatgagtggt attctattcc tgattagact cgcacacaca cacgtacgag
      721 tattaacata aatttgccgg caaataacca aaacaaaacg aacgcaaacg atccgagcac
      781 gtaatgcgag agataagaaa cagaaaagct cgacgtgaaa gaagcttctg gcggaagctc
      841 ttgccgaact ccctataaat acgagacaga aaaccagaat aaatataata acaaactgtt
      901 gaattgtccc atcgattccc agatcaaaag acagaatgtc tctcaccaac aagaacgtcg
      961 ttttcgtggc cggtctgggc ggcattggcc tggacaccag ccgggagttg gtcaagcgta
     1021 atctgaaggt aagagagaac gaattccatg gaatctatgg aatcctaaat ttaaaaattc
     1081 atttatttta gaacctggtc atcctggatc gcattgacaa tccggctgcc attgccgaac
     1141 tgaaggcaat caatcccaag gtgaccatca ccttctatcc ctacgatgtg actgtgcccg
     1201 tcgctgagac caccaagctc ctgaagacca tctttgccca ggtgaagaca atcgatgtcc
     1261 tgatcaacgg tgctggcatc ctggatgatc atcagatcga gcgcaccatt gccgttaact
     1321 acacgggcct ggtcaacacc accacagcca ttctggactt ctgggacaag cgcaagggcg
     1381 gcccaggcgg catcatttgc aacattggct ccgtcaccgg tttcaatgcc atctaccagg
     1441 tgcccgttta ctccggctcc aaggcggcgg tggttaactt tacctcctcc ctggcggtaa
     1501 gaaatcctac aactatccta actatcccaa aactaaatat ttctttgaaa ttccatagaa
     1561 actggctccc attactggtg tcactgctta cactgtcaat cctggcatca ctaagaccac
     1621 tctggtccac aaattcaact cgtggctgga tgtggagccc cgtgtggcgg agaagctgct
     1681 cgagcatccc acccagacct ctcagcagtg cgccgagaac tttgtgaagg ccattgagct
     1741 gaacaagaac ggtgccatct ggaagttgga tttgggcacc ttggagccca tcacatggac
     1801 ccagcactgg gactcgggca tctaaaccat cccagagact ctatgggaca tggcgtttta
     1861 gcttttagtt tctttttttc cactcaattg ttacgtatat acatacacat atggcaataa
     1921 ggctgatttg aaccccgttt tgaatatgat aatacaaaat tatatttgag aaatttcaac
     1981 aaaatcgata agagctatag ctctctttta attaaaaaaa actagataaa aagagcaatg
     2041 gtcagtggtg gtaggcagtg gtgtggtgtg gcctctatcg atttccacac aaaaacttta
     2101 ctgtttagta atagaaaaga acttgagaaa ggcagccaaa agaatgtacg atctgacggg
     2161 taagcatgtc tgctatgtgg ctgactgcgg tggcattgca ctggagacca gcaaggttct
     2221 catgaccaag aatatagcgg tgagtggtag agtggtggtg tagagagtgg agagtggttc
     2281 tggttcttaa acaagtctct agtctctagt ctcttggctg ctgctggtag ctgtcgaaac
     2341 taattaagtt aatgactttt tcatgttttg tttttgtccg ctgacttttg ctgttcaaag
     2401 ctacaattaa gtttatgttt tgagtttgat ctacttttgc ttaagggaag tactttcgat
     2461 tacggattgc tggcatataa aaatagatac caaggaatgt gcatcatatc atattgattc
     2521 tatcttgatt ctttgcccag aaactggcca ttcttcagag cgtggaaaac cccccggcca
     2581 tcgcccagtt acaatcgatt aagcacagca cacagatctt cttctggacc ttcgatgtga
     2641 ccatggcccg cgaggagatg aagaagtact tcgatgaggt catggtccag atggactaca
     2701 tcgatgtgct gatcaatggg gccaccctgt gcgatgagcg gaacattgat gccaccatca
     2761 atacgaatct gaccggcatg atgaacaccg tggccactgt gcttccctat atggaccgta
     2821 agatgggcgg ttcgggtgga ctgatcgtga atgtcacctc cgtcatagga ctggatccat
     2881 cgccagtctt ttgtgcgtac agtgcctcaa agtttggtgt gattgggttc acccgaagtc
     2941 tagcggtgag tgggcgagat cttaacttga aaatgctctc aaatgtcttc cattctttcg
     3001 caggatcccc tgtattacac ccaaaatggt gtggctgtca tggccgtctg ctgtggtccc
     3061 accaaagtgt ttgtcgatcg cgaactgact gccttcctgg catacggcca aacctttgcc
     3121 gatcgcctgc gtcgtgcccc ctgccaatcg acggccgtct gcggcaagaa tatagttaat
     3181 gccatcgaga gatcggagaa cggacagatc tggattgccg acaagggcgg cctggagtcg
     3241 gtggccctcc actggtattg gcatatggcc gatcagtttg tgaactatat gcagagcact
     3301 gacgatgagg atcaggagtt ttttttgggc cagcgatgag gtggaataaa acatcaaatg
     3361 tgtcacaaaa agtaggcaac ttgtttgtgg gtctgggaat agatttgaaa tattttgagg
     3421 tcatatgaat aatgacataa atgagggtgc ctgaaagtat gccaagttt
//