From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Entamoeba histolytica tubulin 1 (tub1) gene, complete cds.
Date: 31 Dec 92 01:30:19 GMT
Distribution: bionet
Checksum: 01766 4

LOCUS       ENHTUBI      1670 bp ds-DNA             INV       16-DEC-1992
DEFINITION  Entamoeba histolytica tubulin 1 (tub1) gene, complete cds.
ACCESSION   L07898
KEYWORDS    GTP binding protein; cytoskeleton protein; tubulin.
SOURCE      Entamoeba histolytica (strain HM-1:IHSS) DNA.
  ORGANISM  Entamoeba histolytica
            Eukaryota; Animalia; Protozoa; Sarcomastigophora; Sarcodina;
            Rhizopoda; Lobosa; Gymnamoeba; Schizopyrenida; Tubulina;
            Entamoebidae.
REFERENCE   1  (sites)
  AUTHORS   Sanchez,M.A., Peattie,D.A., Wirth,D.F. and Orozco,E.
  TITLE     Cloning and characterization of two-tubulin genes which are
            specifically expressed in S and G2 phases during the cell cycle of
            Entamoeba histolytica
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     TATA_signal     204..207
                     /gene="tub1"
     polyA_signal    1617..1623
                     /gene="tub1"
     CDS             228..1595
                     /gene="tub1"
                     /product="tubulin 1"
                     /codon_start=1
                     /translation="MREVITINIGQAGCQLGNKCWELFCLEHGIQPDGTAIANSNEKR
                     SVITGGIDTAYNAFFQELQNGRHVPRAIFVDTEPTVIDEIKTGEYCGLYHPEHLICGK
                     EDASSNFAKGKTGYEPLLNQTMDAIRKATENCTGLQGFFIYNSVGGGTGSGFTAALCE
                     KLADKYTKKTKLNTVIWPSPKLSSGVVEPYNAVLNTHAMMKFVNCTFMVDNESIYKIC
                     QKQLGVHSPSYFHLNQLIAQAMSSITASLRFEGTLNVDLNEFPTNLVPFPRDHFAMMS
                     YAPIVTPEKAIRQTLSVQELTSSLFDPNSLMIQCDDINKGKFMSCCMMYRGDVSSREV
                     NLAVSGIKKQNTVPFVGWCPCSFKCGINSQPATAVPGSSYASTARSACMIANHTAMCQ
                     VFQKVNQNFDLMFGKRAFVHHYVGEGMEENEFTDARQDLYELEVDYANLALDNTIEGE
                     SMTAQ"
BASE COUNT      607 a    240 c    269 g    554 t
ORIGIN      
        1 atccacacag tatcacacta tctgatatac tattcctcat tatatctgta cttataccat
       61 gatattcttt ctattttatc tgaaagttta aataatctaa tcagctctag tcatcaaaaa
      121 ctattgaagg gaaaaataag taaataatct ataagaatca catcataatg ataataagat
      181 tttaactaga acttataaac tattaaatct aaaacctaat cagatctatg cgtgaagtga
      241 ttactattaa tattggtcaa gctggatgtc aacttggaaa taaatgttgg gagttatttt
      301 gccttgaaca tggaattcaa ccagatggaa cagccattgc caattctaat gaaaaaaggt
      361 cagttataac tggaggaata gatactgctt ataatgcctt tttccaagaa ctccaaaatg
      421 gaagacatgt tccaagagct atttttgtcg atactgaacc tactgtaatt gatgaaatta
      481 aaactggaga atattgtgga ctttatcatc ctgaacattt aatttgtggt aaagaagatg
      541 catcctctaa ttttgctaaa ggaaaaacag gatatgaacc attactaaat caaacaatgg
      601 atgctattag aaaagctaca gaaaattgta caggattaca aggatttttc atttataatt
      661 ctgttggtgg aggaactgga agtggtttta ctgcagcatt atgtgagaaa ttagcagaca
      721 aatatacaaa aaagactaaa ttgaacactg ttatttggcc atcaccaaaa ctttctagtg
      781 gagttgttga gccatataat gctgtgttaa atactcatgc tatgatgaaa tttgtaaatt
      841 gtacttttat ggttgataat gaatcaattt ataaaatatg tcaaaaacaa ttaggagttc
      901 atagtccatc atatttccat ttaaatcaat taattgcaca agccatgagt tctattactg
      961 catcattaag atttgaaggt acattaaatg ttgacttaaa tgaatttcca actaatcttg
     1021 ttccattccc aagagaccat tttgctatga tgtcatatgc accaattgtt acaccagaaa
     1081 aagctattag acaaacatta tcagttcaag aacttacatc atcattattt gatccaaata
     1141 gtttaatgat tcaatgtgat gatattaata aaggtaaatt tatgagttgt tgtatgatgt
     1201 atcgaggaga tgtttcatca cgtgaagtta atcttgctgt tagtggaatt aagaaacaaa
     1261 atacagttcc atttgttgga tggtgtccat gttcatttaa atgtggtatt aatagtcaac
     1321 cagctactgc tgttcctggt agttcatatg cttctactgc tagaagtgca tgtatgattg
     1381 ctaaccatac agctatgtgc caagtattcc aaaaagtaaa tcaaaacttt gatttaatgt
     1441 ttggtaaaag agcctttgtt catcattatg ttggagaagg aatggaagaa aatgaattta
     1501 ctgatgcacg acaagacttg tatgaactag aagtagatta tgccaatctt gctcttgaca
     1561 acacaattga aggagaatca atgacagcac aataaattta gacaaacctt tcatttaatt
     1621 aaaattttat tgaaaaaatt tttatcatat ttttataaaa aaaatagatt
//