From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: S.coelicolor genes actIORF1, actIORF2, actIORF3, actVII, actIV and
Date: 21 Dec 92 20:53:11 GMT
Distribution: bionet
Checksum: 38577 12

LOCUS       STMACTI6     5286 bp ds-DNA             BCT       18-NOV-1992
DEFINITION  S.coelicolor genes actIORF1, actIORF2, actIORF3, actVII, actIV and
            actVB for beta-ketoacyl synthase/acyl transferase, acyl carrier
            protein, cyclase/dehydrase, dehydrase and dimerase
ACCESSION   X63449
KEYWORDS    actI gene; actinorhodin; actinorhodin biosynthesis;
            actinorhodin polyketide synthase; acyl carrier protein;
            acyltransferase; condensing enzyme; cyclase; dehydrase; dimerase.
SOURCE      Streptomyces coelicolor DNA.
  ORGANISM  Streptomyces coelicolor
            Prokaryotae; Firmicutes; Streptomycetaceae.
REFERENCE   1  (bases 1 to 5286)
  AUTHORS   Fernandez-Moreno,M.A., Martinez,E., Boto,L., Hopwood,D.A. and
            Malpartida,F.
  TITLE     Nucleotide sequence and deduced functions of a set of
            contranscribed genes of streptomyces coelicolor A3(2) including the
            polyketide synthase for the antibiotic actinorhodin
  JOURNAL   J. Biol. Chem. 267, 19278-19290 (1992)
  STANDARD  full automatic
COMMENT     *source: strain=ssp. A3(2), M145;
               From EMBL    entry SCACTI6;  dated 12-OCT-1992.
FEATURES             Location/Qualifiers
     RBS             93..97
     RBS             1366..1370
     RBS             2616..2620
     RBS             2875..2878
     variation       4665
                     /note="replace(4665,'a')"
                     /partial
     RBS             4724..4727
     CDS             107..1381
                     /gene="actIORF1"
                     /product="Beta-Ketoacylsynthase/Acyl transferase"
                     /codon_start=1
                     /translation="LKRRVVITGVGVRAPGGNGTRQFWELLTSGRTATRRISFFDPSP
                     YRSQVAAEADFDPVAEGFGPRELDRMDRASQFAVACAREAFAASGLDPDTLDPARVGV
                     SLGSAVAAATSLEREYLLLSDSGRDWEVDAAWLSRHMFDYLVPSVMPAEVAWAVGAEG
                     PVTMVSTGCTSGLDSVGNAVRAIEEGSADVMFAGAADTPITPIVVACFDAIRATTARN
                     DDPEHASRPFDGTRDGFVLAEGAAMFVLEDYDSALARGARIHAEISGYATRCNAYHMT
                     GLKADGREMAETIRVALDESRTDATDIDYINAHGSGTRQNDRHETAAYKRALGEHARR
                     TPVSSIKSMVGHSLGAIGSLEIAACVLALEHGVVPPTANLRTSDPECDLDYVPLEARE
                     RKLRSVLTVGSGFGGFQSAMVLRDAETAGAAA"
     CDS             1378..2601
                     /gene="actIORF2"
                     /product="actIORF2"
                     /codon_start=1
                     /translation="MSVLITGVGVVAPNGLGLAPYWSAVLDGRHGLGPVTRFDVSRYP
                     ATLAGQIDDFHAPDHIPGRLLPQTDPSTRLALTAADWALQDAKADPESLTDYDMGVVT
                     ANACGGFDFTHREFRKLWSEGPKSVSVYESFAWFYAVNTGQISIRHGMRGPSSALVAE
                     QAGGLDALGHARRTIRRGTPLVVSGGVDSALDPWGWVSQIASGRISTATDPDRAYLPF
                     DERAAGYVPGEGGAILVLEDSAAAEARGRHDAYGELAGCASTFDPAPGSGRPAGLERA
                     IRLALNDAGTGPEDVDVVFADGAGVPELDAAEARAIGRVFGREGVPVTVPKTTTGRLY
                     SGGGPLDVVTALMSLREGVIAPTAGVTSVPREYGIDLVLGEPRSTAPRTALVLARGRW
                     GFNSAAVLRRFAPTP"
     CDS             2626..2886
                     /gene="actIORF3"
                     /product="Acyl carrier protein"
                     /codon_start=1
                     /translation="MATLLTTDDLRRALVECAGETDGTDLSGDFLDLRFEDIGYDSLA
                     LMETAARLESRYGVSIPDDVAGRVDTPRELLDLINGALAEAA"
     CDS             2883..3833
                     /gene="actIORF4"
                     /product="Cyclase/dehydrase"
                     /codon_start=1
                     /translation="MSRPGEHRVVHTLRTQAPARRLYELVARVEDWPAVFEPTVHVQV
                     LERGPGTERFRIWARVGGRVKTWTSRRTLDPDTLRVTFRQELTQPPIASMGGSWEFRG
                     DGDGTEVVLTHDFAAVDEAALPGLREALDANSGKELAALVALAERRQPPEELVFTFED
                     TLRVPSGDDAYAFIERSDLWQERLPHVRKVTLTEEAAGTGPAETRDMTVQDMTMETVT
                     TDGGTHTTRSIRLCVPARSIVYKQLVPPALLSGHCGAWTFGEDTVTARHTVAIDPARV
                     EEVLGKGATVADARTHLREVLGANSRATLRHAAAAAGPAS"
     CDS             3830..4723
                     /gene="actIORF5"
                     /product="Dehydrase"
                     /codon_start=1
                     /translation="MTVEVREVAEGVYAYEQAPGGWCVSNAGIVVGGDGALVVDTLST
                     IPRARRLAEWVDKLAAGPGRTVVNTHFHGDHAFGNQVFAPGTRIIAHEDMRSAMVTTG
                     LALTGLWPRVDWGEIELRPPNVTFRDRLTLHVGERQVELICVGPAHTDHDVVVWLPEE
                     RVLFAGDVVMSGVTPFALFGSVAGTLAALDRLAELEPEVVVGGHGPVAGPEVIDANRD
                     YLRWVQRLAADAVDRRLTPLQAARRADLGAFAGLLDAERLVANLHRAHEELLGGHVRD
                     AMEIFAELVAYNGGQLPTCLA"
     CDS             4735..5268
                     /gene="actIORF6"
                     /product="Dimerase"
                     /codon_start=1
                     /translation="MAADQGMLRDAMARVPAGVALVTAHDRGGVPHGFTASSFVSVSM
                     EPPLALVCLARTANSFPVFDSCGEFAVSVLREDHTDLAMRFARKSADKFAGGEFVRTA
                     RGATVLDGAVAVVECTVHERYPAGDHIILLGEVQSVHVEEKGVPAVYVDRRFAALCSA
                     AGACPSATGRGVPAHAG"
BASE COUNT      738 a   1925 c   1919 g    704 t
ORIGIN      
        1 ggatccggca tcgaggggtc ccgtatcggc cttcgagcct ccttcgagcc acggggccga
       61 cgatgacgac gaccaccgga cgaacgcatc gaaggagctg ttcggattga agcgcagagt
      121 cgtcatcacg ggcgtcggcg tccgcgcccc cggcgggaac ggcacccggc agttctggga
      181 actgctcacc tcggggcgga cggcgacgcg ccggatctcg ttcttcgacc cctcgcccta
      241 ccgttcacag gtcgcggcgg aggccgactt cgacccggtc gccgagggct tcggcccgcg
      301 ggagctcgac cggatggacc gggcctcgca gttcgccgtg gcctgtgccc gggaggcgtt
      361 cgccgccagc gggctcgacc cggacaccct cgaccccgcc cgggtcgggg tcagcctcgg
      421 cagcgcggtg gcagcggcga ccagcctgga gcgcgagtat ctgctgctgt cggactccgg
      481 ccgtgactgg gaggtcgacg cggcctggct gtcccggcac atgttcgact acctggtgcc
      541 cagcgtcatg ccggccgagg tcgcctgggc ggtcggcgcc gagggcccgg tcacgatggt
      601 ctccaccggc tgcacctcgg gcctggactc cgtcggcaac gcggtccggg cgatcgagga
      661 gggcagcgcc gacgtgatgt tcgcgggagc cgccgacacc ccgatcaccc cgatcgtcgt
      721 cgcctgcttc gacgcgatcc gcgccacgac ggcgcgcaac gacgacccgg agcacgcctc
      781 gcggccgttc gacggtacgc gggacggctt cgtgctggcc gaaggagccg cgatgttcgt
      841 cctggaggac tacgacagcg cgctggcccg cggagcccgc atccacgccg agatctcggg
      901 gtacgcgacg cgctgcaacg cgtaccacat gacgggcctg aaggccgacg gccgcgagat
      961 ggccgagacc atccgggtcg ccctcgacga gtcccgcacg gacgcgacgg acatcgacta
     1021 catcaacgcg cacggctccg gcacccggca gaacgaccgc cacgagacag cggcgtacaa
     1081 gcgcgccctc ggcgaacacg cccggcgcac tccggtcagc tcgatcaagt cgatggtcgg
     1141 ccactcgctg ggcgcgatcg gctcgctgga gatcgcggcc tgcgtactcg ccctcgaaca
     1201 cggcgtggtg cccccgaccg ccaacctgcg caccagcgac cccgagtgcg atctcgacta
     1261 cgttccgctg gaggccaggg agcggaagct gcggtccgta ctcaccgtgg gcagcggctt
     1321 cggcggcttc cagagcgcga tggtgctgcg cgacgccgag accgcggggg cggccgcatg
     1381 agcgtcctca tcaccggagt cggcgtcgtc gcacccaacg gcctcggcct ggcgccgtac
     1441 tggtcggcgg tgctggacgg ccgccacggc ctcggcccgg tcacccgctt cgacgtgagc
     1501 cggtacccgg cgaccctcgc cggccagatc gacgacttcc acgcgccgga ccacatcccg
     1561 ggccggttgc tgccgcagac ggacccctcc acacggctcg cgctgaccgc cgcggactgg
     1621 gccctccagg acgcgaaggc ggacccggag tcgctgaccg actacgacat gggcgtggtg
     1681 accgccaacg cctgcggtgg gttcgacttc acccaccggg agttccgcaa gctgtggtcc
     1741 gagggcccca agtcggtcag cgtgtacgag tccttcgcct ggttctacgc ggtgaacacc
     1801 ggccagatct ccatccggca cggtatgcgc ggtccgagca gtgccctggt cgccgagcag
     1861 gccgggggcc tggacgccct gggccacgcc cgccgtacga tccgccgcgg cacgccgctg
     1921 gtggtctccg gcggggtgga ctcggcactc gacccgtggg gctgggtctc gcagatcgcc
     1981 agtggccgga tcagcacggc caccgatccg gaccgggcgt atctgccctt cgacgagcgg
     2041 gcggccggct acgttccggg cgagggcggg gccatcctcg tcctggagga cagcgccgcg
     2101 gccgaggccc gcggccggca cgacgcgtac ggcgaactcg ccggctgcgc ctccaccttc
     2161 gaccccgccc ccggatccgg acggcccgcg ggcctggaac gcgcgatccg gctggcgctg
     2221 aacgacgccg ggaccggtcc cgaggacgtc gacgtcgtgt tcgccgacgg cgcgggcgtg
     2281 cccgagctgg acgccgccga ggcccgggcg atcggccggg tgttcggccg cgaaggggtg
     2341 cccgtcaccg tccccaagac gacgacgggc cgcctctact ccggcggcgg cccgctcgac
     2401 gtggtcaccg ccctgatgtc cctgcgcgaa ggggtgatcg cgccgaccgc gggcgtgacg
     2461 tccgtacccc gggagtacgg catcgacctg gtgctcggcg aaccccgcag cacggccccg
     2521 cgcaccgcac tggtcctggc caggggccgc tggggcttca actcggcggc ggtcctgcgc
     2581 cgtttcgcac cgaccccgta acgaccccga gaactggaga aagccatggc aaccctgctg
     2641 accaccgacg atctgcgccg cgccctcgtg gagtgcgccg gtgagacgga cgggacggac
     2701 ctgtccggcg acttcctcga cctccgcttc gaggacatcg ggtacgactc gctcgccctg
     2761 atggagaccg cggcgcgact cgagagccgg tacggcgtgt ccatacccga cgacgtcgcc
     2821 ggccgcgtcg acacgccgcg agagctgctc gacctgatca acggcgcact ggccgaggcg
     2881 gcatgagccg gccgggagaa caccgggtgg tccacaccct gaggacccag gccccggcgc
     2941 ggcggctcta cgagctggtc gcacgcgtcg aagactggcc ggccgtcttc gaacccaccg
     3001 tgcacgtaca ggtcctggag cgcggcccgg gcaccgaacg cttccggatc tgggcgcgcg
     3061 tgggcggccg ggtgaagacc tggacgtccc gccggacgct ggatcccgac accctgaggg
     3121 tgacgttccg ccaggagctc acccagccgc ccatcgcctc gatgggcggg agctgggagt
     3181 tccggggcga cggcgacggc accgaagtcg tgctgaccca cgacttcgcc gcggtcgacg
     3241 aagccgccct gccgggactg cgcgaggcgc tggacgcgaa cagcgggaag gagctggccg
     3301 cgctggtcgc gctggcggag cgccggcagc cgcccgagga gctggtcttc acgttcgagg
     3361 acacgctccg ggtgccgtcc ggggacgacg cctacgcctt catcgagcgc agcgacctgt
     3421 ggcaggaacg gctgccgcac gtgcggaagg tgacactcac cgaggaggcg gcgggtaccg
     3481 gccccgccga gacgcgggac atgaccgtcc aggacatgac catggagacg gtgaccaccg
     3541 acggcggtac ccacaccacc cgttcgatcc ggctctgcgt ccccgcgagg agcatcgtgt
     3601 acaagcagct cgtcccgccc gccctgctct ccgggcactg cggcgcctgg accttcggcg
     3661 aggacacggt caccgcccgc cacaccgtgg cgatcgaccc ggcccgcgtg gaagaggtcc
     3721 tgggcaaagg cgcgaccgtc gcggacgccc gtacccatct gcgcgaggtg ctcggtgcca
     3781 acagccgggc cacgctccgc cacgcggcgg ccgccgccgg gccggcgtca tgacggtcga
     3841 ggtccgtgag gtcgccgagg gtgtctacgc ctacgagcag gcccccggcg gctggtgcgt
     3901 cagcaacgcg ggcatcgtgg tgggcggcga cggcgcgctc gtcgtcgaca cgctgtcgac
     3961 gatcccgagg gcgcggcggc tggcggagtg ggtcgacaag ctcgccgcgg gccccggccg
     4021 taccgtggtc aacacccact tccacggcga ccacgccttc ggcaaccagg tcttcgcgcc
     4081 ggggacgcgg atcatcgcgc acgaggacat gcggtccgcc atggtgacga ccggcctggc
     4141 cctcaccggg ctctggccgc gggtcgactg gggcgagatc gagctgaggc cgccgaacgt
     4201 gaccttccgc gaccggttga ccctgcacgt cggcgagcga caggtcgaac tgatctgcgt
     4261 cggccccgcg cacaccgatc acgacgtggt ggtctggctg cccgaggagc gggtgctgtt
     4321 cgcgggggac gtcgtcatgt cgggcgtcac tccgttcgcc ctcttcggtt cggtggccgg
     4381 cacgctggcc gcgctcgacc ggctggcgga gctggagccc gaggtggtcg tcggcgggca
     4441 cggcccggtg gcgggacccg aggtgatcga cgccaaccgg gactatctgc gctgggtcca
     4501 gcgcctcgcc gccgatgcgg tcgaccgccg gctgacaccg ttgcaggccg cgcgccgggc
     4561 ggacctcggc gccttcgccg ggctgctgga cgcggagcgc ctcgtcgcga acctgcaccg
     4621 ggcccacgag gagctgctgg gcgggcacgt gcgtgacgcc atggagatct tcgcggagct
     4681 ggtcgcctac aacggtgggc agctgcccac ctgcctcgcc tagggagttc gaagatggca
     4741 gccgaccagg gaatgctccg ggacgccatg gcccgggtgc cggccggggt ggcgctcgtc
     4801 accgcccatg accgcggggg agtcccgcac ggtttcaccg ccagttcgtt cgtgtccgtc
     4861 tcgatggagc cgccactggc actggtctgc ctggctcgta cggccaactc cttcccggtg
     4921 ttcgacagtt gcggcgagtt cgcggtgagc gtgctgcgcg aggaccacac ggacctggcc
     4981 atgcgcttcg cgcgcaagtc cgcggacaag ttcgcgggcg gggagttcgt ccgtaccgcg
     5041 cggggagcga ccgtgctcga cggagcggtc gcggtcgtcg agtgcacggt ccacgagcgc
     5101 tacccggcgg gcgaccacat catcctgctc ggcgaggtcc agtccgtgca cgtcgaggag
     5161 aagggcgtac cggcggtcta cgtggaccgc cggttcgccg ccctgtgctc ggcggcgggt
     5221 gcctgcccgt ccgccaccgg gcggggcgtg cccgcgcatg ccggctgagg ccggccggga
     5281 aggccg
//