From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Aequorea victoria green-fluorescent protein mRNA, complete cds.
Date: 7 Apr 92 10:01:20 GMT
Distribution: bionet
Checksum: 07385     9

LOCUS       AEVGFPB      5170 bp ss-mRNA            INV       07-APR-1992
DEFINITION  Aequorea victoria green-fluorescent protein mRNA, complete cds.
ACCESSION   M62654
KEYWORDS    aequorin associated energy-transfer acceptor; bioluminescence;
            green-fluorescent protein; photocytes.
SOURCE      Aequorea victoria (library: lambda gt10) cDNA to mRNA.
  ORGANISM  Aequorea victoria
            Eukaryota; Animalia; Eumetazoa; Cnidaria; Hydrozoa; Hydroida;
            Leptomedusae; Aequoreidae.
REFERENCE   1  (bases 1 to 5170)
  AUTHORS   Prasher,D.C., Eckenrode,V.K., Ward,W.W., Prendergast,F.G. and
            Cormier,M.J.
  TITLE     Primary structure of the Aequorea victoria green-fluorescent
            protein
  JOURNAL   Gene 111, 229-233 (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     mRNA            join(198..413,946..1240,2308..>2744)
                     /gene="GFP"
                     /partial
     CDS             join(208..413,946..1240,2308..2523)
                     /product="green-fluorescent protein"
                     /gene="GFP"
                     /codon_start=1
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFYKD
                     DGNYKSRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKMEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHM
                     ILLEFVTAAGITHGMDELYK"
     intron          <1..197
                     /number=1
                     /gene="GFP"
     misc_feature    193..201
                     /note="fluorescent chromophore"
                     /gene="GFP"
                     /evidence=EXPERIMENTAL
     exon            198..413
                     /number=2
                     /gene="GFP"
     intron          414..945
                     /number=2
                     /gene="GFP"
     exon            946..1240
                     /number=3
                     /gene="GFP"
     intron          1241..2307
                     /number=3
                     /gene="GFP"
     exon            2308..>2744
                     /number=4
                     /gene="GFP"
BASE COUNT     1830 a    824 c    836 g   1679 t      1 others
ORIGIN
        1 aagcttcaaa ttaagtcagc tccttaaatg aaagataata aagtgtagtt caagaactat
       61 atgaatgatg tgttttcaga taaccaaaat ggggaaaaac atgctaaagt cagcatattt
      121 ttggaaaatt gatgacgtca tcatgacgtc gttttgatga caaaacttat tataagcgaa
      181 ttcttatatt tttacaggat aacaaagatg agtaaaggag aagaactttt cactggagtt
      241 gtcccaattc ttgttgaatt agatggtgat gttaatgggc acaaattctc tgtcagtgga
      301 gagggtgaag gtgatgcaac atacggaaaa cttaccctta aatttatttg cactactgga
      361 aagctacctg ttccatggcc aacacttgtc actactttct cttatggtgt tcagtaagtg
      421 cattttatac tcttttaata tcagtgttaa gaaaatcaag tgtcttgcta ttttttcgat
      481 tattggtgca attctagtca aattattgcg tttttttacc caaaatgtta atgtaaaact
      541 gaaatttggc acacttgcgc aaatatatac agggtatttt gaaaaaatta aacaggatga
      601 taaaagttgc acagaaactt atctcaagat ttacccgcag aaagatgctt naaaaattga
      661 tatttgacag agcaaaacct gagattcacg tcttttagtt gtttgacttg aaattttggt
      721 gacaggtagg tatcatgaaa aacaaacaaa acgtaaaaat atcacgtgat taaagtgtat
      781 cttacagacc agaaacagtt ttattaactt ctattattct attttgcaat atacacattg
      841 tatcaatttc ttgagttact cgaagtaata ccgacctatc atcagaattt caagtcaaca
      901 caacattata tggggctgat tagggaatga ttttgtctct tttagatgct tttcaagata
      961 cccagatcat atgaaacagc atgacttttt caagagtgcc atgcccgaag gttatgtaca
     1021 ggaaagaact atattttaca aagatgacgg gaactacaaa tcacgtgctg aagtcaagtt
     1081 tgaaggtgat accctcgtta atagaattga gttaaaaggt attgatttta aagaagatgg
     1141 aaacattctt ggacacaaaa tggaatacaa ctataactca cacaatgtat acatcatggc
     1201 agacaaacaa aagaatggaa tcaaagttaa cttcaaaatt gtatgtatac gttaagggca
     1261 taaatttttg cgggcataaa atcttgcgaa atttattatc gcgaataggt tacgcaaaat
     1321 ctataattaa aatgtatttt tttctgctga ttttctaaat aacaactcaa cccgtcattt
     1381 ttatatcgca aaaataaatt ccgaaataat ttatgctcgc aaaaatttag gcccataagt
     1441 agacttttga tatctgcgtg ctctgcaatg aagtaaaaat acgatatttt cattgaaata
     1501 cacgggttca aagttatttg ttaattcaat aagcgtgcgc agaaattaaa ggacgtataa
     1561 agatacgaac acatcaaacc attcatgcgt aaataatgtt ctatttttaa aattcaccaa
     1621 agcttaaata ttcttaagaa ttattcatgt gccatgggag caacaatata gttatggaca
     1681 aaaatttctg agttcacttt tatttctgcg cgcccgcatc aaagttcaaa caactgtgaa
     1741 cccgagtttt ttccagcttg caattttaat aagagacaaa aagcaaattg cagttcaaga
     1801 aaatcgagat attgccagat gtaaacattt aataagagac aaaaagttca taagcgttct
     1861 aaagaacagc aacaaaataa taattagaat taaacgagtt ctcaaacaaa ataaaaactg
     1921 aagtcaaaga gtcagtaagg aatttagtta acgatgcttt ataatcaaag ttttaattcc
     1981 agttcatgta tgcaattaac aataagatct tggagaattg aatatgtttc gaaattttat
     2041 aaattcggat ttaatttcta aagttgtgta tcaaaaatag ttcaaactat tttcatgaaa
     2101 agatgataaa ttacggtaat aagtatataa tataatcaat taaaattaat tttaggctca
     2161 aattacagaa tccacgtttt ttttctctag acatagcaca gtgtttagat gtttgtttta
     2221 tttcatccat ccttattaca gttttcctct gaactttaat actagcgtac aatttgaata
     2281 ataatctgaa atgattcaac ttttcagaga cacaacattg aagatggaag cgttcaacta
     2341 gcagaccatt atcaacaaaa tactccaatt ggcgatggcc ctgtcctttt accagacaac
     2401 cattacctgt ccacacaatc tgccctttcc aaagatccca acgaaaagag agatcacatg
     2461 atccttcttg agtttgtaac agctgctggg attacacatg gcatggatga actatacaaa
     2521 taaatgtcca gacttccaat tgacactaaa gtgtccgaac aattactaaa atctcagggt
     2581 tcctggttaa attcaggctg agatattatt tatatattta tagattcatt aaaattttat
     2641 gaataattta ttgatgttat taataggggt tattttctta ttaaataggc tactggagtg
     2701 cattcctaat tctatattaa ttacaatttg atttgacttg ctcagaatcc cgcttcattg
     2761 cttttccact tgcattatcc ttatttagta ttaatttgta ttttggtttg gctacattga
     2821 gtgcaaaaaa cctaattttc ggacgaattt tcgaacgaat ttttttgacg gaattttctt
     2881 cattctattt actcctctag ctaaattatt ttaccttttt gttaatttgg ttaaattatt
     2941 ctctgagccg atgattgaga aattaatgga ttaaaagtga gtaccttaca tgttgtcaac
     3001 ttgtaacgaa tggaaaaaga aattacgttt caagagtttg aaaggtaata cagttacagt
     3061 taaccgcaga aaaattgcat gatgattgat aaattcgatt tttgttatcc taaaattttc
     3121 caaacgtcag tggccgacga ctttatcagg gacttctaaa agtgaaaaat aatcaggtgc
     3181 ggatttcgaa ggcgcaaaac tataggaaga gagcgaaatg tcattaaatt atcatattct
     3241 attaactgat gacaatagat gatgaaaagt ttatgattat tcactctcct cctgtaatta
     3301 tgcgaccctt ctagattcac gcctgaaagt atagctacct gggatgaagt actagtctga
     3361 ggactcttca cctaaaaatt aaattcttat aagagtaaac aagaaactta gcagttacaa
     3421 acgggagagc gatgagaaac aaaaacaatt acgttgccac tatgaatatc gatgttcaat
     3481 caattttgtt ccttacttat aagaacgaga tcgtcttaac ttaaaatagt aaaatgttat
     3541 caagataata gcaatttttt accgacacag cgaagactca ctactgaaat gatcagtttt
     3601 aatcaggcaa ataatccgtg gcacataata gtgaccgaaa ataattaatc ggcattaaga
     3661 ctaccgaaat aataatgttt tttctactgc gtatacgcgt gagaaatttt caataagctc
     3721 atcatcttca gcatagttat acttttatgt aaagtatcaa ttccgacata aaataacggc
     3781 ttattatcga aataatagcg ttttctctac tccatgcgcg tcaaaagttc tctctaggct
     3841 catcatcttc agcataatta taatttttgt aaagtaccag ttccggtcga aaataatgac
     3901 taattaccga aattatagtg tttttctatt gccatgcgcg tgaaaaattt tgattgaatc
     3961 atcatcttca gcataggcat aattctttgt aaaatatcga ttccgacata aaataatggc
     4021 ctattaccga aataatcgcg tttttcctac tgcgcatgcg cgtcaaaaat tatattttta
     4081 ttcatcatct tcagcataat tatatttttt tgtaaagtac cagttccggt agaaaataat
     4141 gacttgttac tgaaataata gcgtttttct attgcgcatg cgctataaaa attaaagtaa
     4201 cgtcatcata ttcagcatgg tattgaaatt ttcaaattta attaacctat tgaacaagaa
     4261 tgtacacttg catcaaaata ggtgaaattc gccaatatcg ctaaatgtga cgcgcgggag
     4321 caatactacg catgtagctt caggtaaagc atgtagaaac tcggaggagt aggagtccac
     4381 cgtcgaaact aaaacgggat acactacgct atggccttcg ctctcccgta aaaagggact
     4441 aacaatacga cctaattgaa atactaaaaa aaacaagaga aatttaaccc ctttgttaac
     4501 acttttcaaa agtgggattt tttagccaac catctggtat atatggttgc tcattttatt
     4561 attatctctt tctttattgt tggtacaacg tagtcaaaat acaaattagg ttaataaaaa
     4621 gcaacattat aatgtataaa atctaattgt gtctaattac cgacaaattt tacaggaaca
     4681 gttttcacca gaccgagtct taattttagt tttaaaagaa attatgtttc tactgttctg
     4741 acaatctgaa gacaattagt tctagtgtaa caatgctctg aattgaatat attcagcaat
     4801 attttgtttg taagaattgg atgaatgtac gaaccttcag cagatttata ccaagtgtta
     4861 gatttaacaa gatttgcaag ctgatgagtt tcgagaaaat tcaacatatc tggatttgag
     4921 ggtggaacat taaaatctcc taagataata attctatcat aattagaata taaattatca
     4981 atgatgtcat ttaagtgatc tagaaaaata ttgatagtaa cagttggatg tttgtatata
     5041 gaaatagtaa gccatctatt tttcccaaat gcgagttcaa aaaccaaaat tggattcctt
     5101 caaagaaaaa agacattaag aaacttgatg gaatcccttc tcgactgtaa acaagcagtc
     5161 tctgggatcc
//