From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Thermus aquaticus YT-1 gene for aqualysin I.
Date: 28 Jul 92 10:08:06 GMT
Distribution: bionet
Checksum: 20060     4

LOCUS       TTHAQUALT    1105 bp ds-DNA             BCT       28-JUL-1992
DEFINITION  Thermus aquaticus YT-1 gene for aqualysin I.
ACCESSION   X07734
KEYWORDS    aqualysin I; protease; serine protease.
SOURCE      Thermus aquaticus.
  ORGANISM  Thermus aquaticus
            Prokaryotae; Gracilicutes; Scotobacteria; Neisseriaceae.
REFERENCE   1  (bases 1 to 1105)
  AUTHORS   Kwon,S.-T., Terada,I., Matsuzawa,H. and Ohta,T.
  TITLE     Nucleotide sequence of the gene for aqualysin I (a thermophilic
            alkaline serine protease) of Thermus aquaticus YT-1 and
            characteristics of the deduced primary structure of the enzyme
  JOURNAL   Eur. J. Biochem. 173, 491-497 (1988)
  STANDARD  simple staff_entry
COMMENT     The deduced amino acid sequence agrees with the NH2-terminal amino
            acid sequence reported by Matsuzawa et al. in Eur.J.Biochem., 1988,
            171:441-447.
FEATURES             Location/Qualifiers
     sig_peptide     <1..>1105
                     /note="aqualysin I signal peptide"
                     /codon_start=1
     CDS             <1..>1105
                     /product="aqualysin I"
                     /gene="YT-1"
                     /codon_start=1
                     /translation="LQARLAPQGVVVTQAYTGALQGFAAEMAPQALEAFRQSPDVEFI
                     EADKVVRAWATQSPAPWGLDRIDQRDLPLSNSYTYTATGRGVNVYVIDTGIRTTHREF
                     GGRARVGYDALGGNGQDCNGHGTHVAGTIGGVTYGVAKAVNLYAVRVLDCNGSGSTSG
                     VIAGVDWVTRNHRRPAVANMSLGGGVSTALDNAVKNSIAAGVVYAVAAGNDNANACNY
                     SPARVAEALTVGATTSSDARASFSNYGSCVDLFAPGASIPSAWYTSDTATQTLNGTSM
                     ATPHVAGVAALYLEQNPSATPASVASAILNGATTGRLSGIGSGSPNRLLYSLLSSGSG
                     STAPCTSCSYYTGSLSGPGDYNFQPNGTYYYSPA"
     mat_peptide     160..>1105
                     /note="aqualysin I"
                     /codon_start=1
BASE COUNT      193 a    370 c    340 g    202 t
ORIGIN
        1 ctgcaggcac ggctggctcc tcagggggta gtggtgaccc aggcctacac gggcgccctc
       61 cagggatttg cggcggagat ggcgccccag gccttagagg cctttaggca gagtcccgac
      121 gtggagttca tagaggcgga caaggtggta cgggcctggg ctacccagag cccggctcct
      181 tggggcctgg accggattga ccagcgggac cttccccttt ccaacagcta cacctacacc
      241 gctacgggaa ggggggttaa cgtctatgtg attgacaccg gaatccgcac gacccaccgg
      301 gagttcggcg gccgggcccg ggtaggctat gacgccttag gggggaacgg ccaggactgc
      361 aacggccacg gtacccatgt ggcgggaacg atcggcggag tgacctatgg ggtagccaaa
      421 gcggttaacc tctacgctgt gcgcgtcctg gactgcaacg gttccggctc cacctctggg
      481 gtcatcgctg gggtggactg ggtcacgcgg aaccaccgca ggccggccgt tgccaacatg
      541 agcttaggag gcggagtctc cactgccctg gacaacgccg tgaagaactc catcgccgcg
      601 ggagtggtct acgctgtggc tgcggggaac gacaacgcca acgcctgcaa ctactcccca
      661 gcccgggtgg ccgaggccct taccgtgggc gctaccacat cttccgacgc ccgtgccagc
      721 ttctccaact acggtagttg cgtggacctc ttcgcccctg gggcttccat tccctcggcg
      781 tggtacacct cggacacggc cacccagacc cttaacggca cctccatggc caccccccat
      841 gtggccgggg tggctgcttt gtatctagag caaaatcctt cggctacgcc ggcctctgtt
      901 gctagcgcta tcctcaacgg agccactacg gggcggcttt cggggatcgg atcggggtcc
      961 cccaaccgtc tcctttactc cctgctctcc tcggggagtg gttccacagc cccctgtacc
     1021 agctgtagct actacacggg cagcctttcc ggtcctggtg actataactt ccaacccaac
     1081 ggcacctact actacagccc tgcag
//