From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Plasmid R1033 from Pseudomonas aeruginosa cmlA gene, complete cds,
Date: 26 Jan 93 05:50:16 GMT
Distribution: bionet
Checksum: 34753 6

LOCUS       R10CMLA      2141 bp ds-DNA             BCT       25-JAN-1993
DEFINITION  Plasmid R1033 from Pseudomonas aeruginosa cmlA gene, complete cds,
            and streptomycin/spectinomycin 3''-adenyltransferase gene, 3' end.
ACCESSION   M64556
KEYWORDS    streptomycin-spectinomycin resistance gene;
            streptomycin/spectinomycin adenyltransferase.
SOURCE      Plasmid R1033 from Pseudomonas aeruginosa DNA.
  ORGANISM  Plasmid R1033
            Prokaryotae.
REFERENCE   1  (bases 1 to 2141)
  AUTHORS   Bissonnette,L., Champetier,S., Buisson,J.-P. and Roy,P.H.
  TITLE     Characterization of the nonenzymatic chloramphenicol resistance
            (cmlA) gene of the In4 integron of Tn1696: Similarity of the
            product to transmembrane transport proteins
  JOURNAL   J. Bacteriol. 173, 4493-4502 (1991)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     misc_recomb     394..396
                     /organism="'Pseudomonas aeruginosa, beginning of
                     integrase-mediated cmlA cassette insertion'"
                     /note="putative"
     -35_signal      416..421
                     /note="'p1, probable promoter'; putative"
     -35_signal      428..433
                     /note="'p2, possible alternate promoter'; putative"
     -35_signal      435..440
                     /note="'p3, possible alternate promoter'; putative"
     -10_signal      439..444
                     /note="'p1'; putative"
     -10_signal      450..455
                     /note="'p2'; putative"
     -10_signal      458..463
                     /note="'p3'; putative"
     RBS             588..592
                     /note="putative"
     misc_recomb     1943..1945
                     /organism="'Pseudomonas aeruginosa, end of cmlA cassette
                     insertion'"
                     /note="putative"
     CDS             <1..339
                     /gene="aadA2"
                     /note="putative"
                     /codon_start=1
                     /translation="EFFDPVPEQDLFEALRETLKLWNSQPDWAGDERNVVLTLSRIWY
                     SAITGKIAPKDVAADWAIKRLPAQYQPVLLEAKQAYLGQKEDHLASRADHLEEFIRFV
                     KGEIIKSVGK"
     CDS             601..1860
                     /gene="cmlA"
                     /note="putative"
                     /product="chloramphenicol resistance protein"
                     /codon_start=1
                     /translation="MSSKNFSWRYSLAATVLLLSPFDLLASLGMDMYLPAVPFMPNAL
                     GTTASTIQLTLTTYLVMIGAGQLLFGPLSDRLGRRPVLLGGGLAYVVASMGLALTSSA
                     EVFLGLRILQACGASACLVSTFATVRDIYAGREESNVIYGILGSMLAMVPAVGPLLGA
                     LVDMWLGWRAIFAFLGLGMIAASAAAWRFWPETRVQRVAGLQWSQLLLPVKCLNFWLY
                     TLCYAAGMGSFFVFFSIAPGLMMGRQGVSQLGFSLLFATVAIAMVFTARFMGRVIPKW
                     GSPSVLRMGMGCLIAGAVLLAITEIWALQSVLGFIAPMWLVGIGVATAVSVAPNGALR
                     GFDHVAGTVTAVYFCLGGVLLGSIGTLIISLLPRNTAWPVVVYCLTLATVVLGLSCVS
                     RVKGSRGQGEHDVVALQSAGSTSNPNR"
     CDS             2052..>2141
                     /gene="ORF4"
                     /note="putative"
                     /codon_start=1
                     /translation="MKGWLFLVIAIVGEVIATSALKSSEGFTKL"
BASE COUNT      447 a    529 c    596 g    569 t
ORIGIN      
        1 gaattctttg acccggttcc tgaacaggat ctattcgagg cgctgaggga aaccttgaag
       61 ctatggaact cgcagcccga ctgggccggc gatgagcgaa atgtagtgct tacgttgtcc
      121 cgcatttggt acagcgcaat aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg
      181 gcaataaaac gcctacctgc ccagtatcag cccgtcttac ttgaagctaa gcaagcttat
      241 ctgggacaaa aagaagatca cttggcctca cgcgcagatc acttggaaga atttattcgc
      301 tttgtgaaag gcgagatcat caagtcagtt ggtaaatgat gtctaacaat tcgttcaagc
      361 cgaccgcgct acgcgcggcg gcttaactcc ggcgttgggc gcacaataag gctccttgca
      421 gagttgcttg aaagttgtta cgattcaaat tcaatcatga gatagtcagc agatgagcac
      481 ttccaagaac gcagacaagt aagccgcagc aaccttcatt tttcggttgt tgcggcgttc
      541 tcatgaatcc ttttgctcta cgggagcgcc gccaaatcct ttgttcaagg agatggtttc
      601 gtgagctcaa aaaactttag ttggcggtac tcccttgccg ccacggtgtt gttgttatca
      661 ccgttcgatt tattggcatc actcggcatg gacatgtact tgccagcagt gccgtttatg
      721 ccaaacgcgc ttggtacgac agcgagcaca attcagctta cgctgacaac gtacttggtc
      781 atgattggtg ccggtcagct cttgtttgga ccgctatcgg accgactggg gcgccgcccc
      841 gttctactgg gaggtggcct cgcctacgtt gtggcgtcaa tgggcctcgc tcttacgtca
      901 tcggctgaag tctttctggg gcttcggatt cttcaggctt gtggtgcctc ggcgtgcctt
      961 gtttccacat ttgcaacagt acgtgacatt tacgcaggtc gcgaggaaag taatgtcatt
     1021 tacggcatac tcggatccat gctggccatg gtcccggcgg taggcccatt gctcggagcg
     1081 ctcgtcgaca tgtggcttgg gtggcgggct atctttgcgt ttctaggttt gggcatgatc
     1141 gctgcatctg cagcagcgtg gcgattctgg cctgaaaccc gggtgcaacg agttgcgggc
     1201 ttgcaatggt cgcagctgct actccccgtt aagtgcctga acttctggtt gtacacgttg
     1261 tgttacgccg ctggaatggg tagcttcttc gtctttttct ccattgcgcc cggactaatg
     1321 atgggcaggc aaggtgtgtc tcagcttggc ttcagcctgc tgttcgccac agtggcaatt
     1381 gccatggtgt ttacggctcg ttttatgggg cgtgtgatac ccaagtgggg cagcccaagt
     1441 gtcttgcgaa tgggaatggg atgcctgata gctggagcag tattgcttgc catcaccgaa
     1501 atatgggctt tgcagtccgt gttaggcttt attgctccaa tgtggctagt gggtattggt
     1561 gtcgccacag cggtatctgt ggcgcccaat ggcgctcttc gaggattcga ccatgttgct
     1621 ggaacggtca cggcagtcta cttctgcttg ggcggtgtac tgctaggaag catcggaacg
     1681 ttgatcattt cgctgttgcc gcgcaacacg gcttggccgg ttgtcgtgta ctgtttgacc
     1741 cttgcaacag tcgtgctcgg tctgtcttgt gtttcccgag tgaagggctc tcgcggccag
     1801 ggggagcatg atgtggtcgc gctacaaagt gcgggaagta catcaaatcc caatcgttga
     1861 gagaatgtgg caagctatcg cccaacaaat cgctgcagcc gacccaaaac cgctacgcgg
     1921 tttcggtcgg ctgagctcag gcgttagatg cactaagcac ataattgctc acagccaaac
     1981 tatcaggtca agtctgcttt tattattttt aagcgtgcat aataagccct acacaaattg
     2041 ggagatatat catgaaaggc tggctttttc ttgttatcgc aatagttggc gaagtaatcg
     2101 caacatccgc attaaaatct agcgagggct ttactaagct t
//