From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Trichomonas vaginalis beta-tubulin (btub1) gene, 5' end cds.
Date: 6 Nov 92 18:36:05 GMT
Distribution: bionet
Checksum: 29872 4

LOCUS       TRITUBA      1530 bp ds-DNA             INV       02-NOV-1992
DEFINITION  Trichomonas vaginalis beta-tubulin (btub1) gene, 5' end cds.
ACCESSION   L05468
KEYWORDS    beta-tubulin; tubulin.
SOURCE      Trichomonas vaginalis (strain Tv1:MCP) (library: lambda-EMBL3) DNA.
  ORGANISM  Trichomonas vaginalis
            Eukaryota; Animalia; Protozoa; Sarcomastigophora; Mastigophora;
            Kinetoplastida; Trichomonadida; Trichomonadidae.
REFERENCE   1  (bases 1 to 1530)
  AUTHORS   Katiyar,S.K. and Edlind,T.D.
  TITLE     Beta-tubulin genes of Trichomonas vaginalis
  JOURNAL   Unpublished (1993)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     5'UTR           235..244
                     /gene="btub1"
                     /evidence="EXPERIMENTAL"
                     /citation=[1]
     CDS             245..>1530
                     /note="btub1 DNA clone extended to BglII site at position
                     1492; remaining 3' segment encoding COOH terminus
                     sequenced from reverse PCR product"
                     /note="putative"
                     /citation=[1]
                     /gene="btub1"
                     /product="beta-tubulin"
                     /codon_start=1
                     /translation="MVREIVHIQAGQCGNQIGAKFWEVISDEHGIDPTGSYHRDSDLQ
                     LERINVYYNEATGAKYVPRAILVDLEPGTSESVRAGQFGQLFRPDNFVFGQSGAGNNW
                     AKGYYTEGQELCESILDVIRKEAESCDCLQGFQLVHSLGGGTGAGLGTLLLNKLREEY
                     PDRILSTYSIVPSPKVSDTVVEPYNCTLSVHQLVESADEVFCIDNEALYDICFRTLKL
                     TTPTYGDLNHLVSMVMSGTTCALRFPGQLNSDLRKLAVNLVPFPRLHFFIVGFAPLTS
                     RGSQQYRALTVPELTSQLFDNKNMMAACDPRRVSYLTSAHFRGRMSSKEVDEQMLNIQ
                     ARNTSYFVEWIPSNVKSAICDIPPRGLKMAATFIGNTTAFRELFTRVDSQFQKMYARR
                     AFIHWYVNEGLETVEFDEARSNMTDLIQEYEMYETAG"
BASE COUNT      383 a    451 c    298 g    398 t
ORIGIN      
        1 gatcacaaaa acattcattc aataaattct ttggcatact taaattctta gaatgaaggg
       61 ttattttgtg aaaatttcaa atctgaaaaa tcaacacgtg gaattcgatt tcttccatta
      121 tcgtttcatt atattcatgg taaatagtat tgctatcatg tttatttcgt ttaaaaaagt
      181 cttaacggac ggcattcaat taattgataa gtacgatatg atacgttatt aatcacaact
      241 cttcatggtt cgtgaaatcg ttcacatcca agctggacag tgcggtaacc aaattggtgc
      301 caagttctgg gaagtcatct ccgacgaaca cggcattgac ccaacaggct cctaccaccg
      361 cgactccgat cttcaactcg agcgcatcaa cgtttactac aacgaagcca caggcgccaa
      421 gtacgtccca cgtgctatcc ttgtcgacct tgaaccaggc acaagtgagt ccgtccgtgc
      481 cggccagttc ggtcagcttt tccgcccaga taacttcgtc ttcggccaat ccggtgctgg
      541 taacaactgg gctaagggct actacacaga aggccaggaa ctttgcgaat ccatcctcga
      601 tgtcatccgt aaggaagctg aatcctgcga ctgccttcag ggcttccagc ttgtccactc
      661 cctcggcggt ggcacaggcg ctggtctcgg cacactcctt ctcaacaagc tccgtgaaga
      721 gtacccagat cgtatcctct ccacatactc catcgtccca tctccaaagg tttctgatac
      781 agtcgtcgag ccatacaact gcacactctc cgtccaccag ctcgtcgaat ctgctgatga
      841 agttttctgc attgataacg aagctcttta cgatatctgc ttccgtacac tcaagctcac
      901 aacaccaaca tacggcgatc ttaaccacct tgtttccatg gttatgtccg gcacaacatg
      961 cgctctccgc ttcccaggtc agctcaactc cgaccttcgt aagctcgctg tcaaccttgt
     1021 tccattccca cgtctccact tcttcatcgt tggcttcgct ccactcacat cccgtggctc
     1081 ccagcagtac cgcgctctta cagtcccaga gctcacatcc cagcttttcg ataacaagaa
     1141 catgatggct gcttgcgatc cacgccgtgt gtcttacctt acatccgctc acttccgtgg
     1201 ccgtatgtca tctaaggaag tcgatgagca gatgcttaac atccaggctc gtaacacatc
     1261 ctacttcgtc gagtggatcc catccaacgt caagtcagct atctgcgata tcccaccacg
     1321 tggtcttaag atggctgcta cattcatcgg caacacaaca gccttccgtg aactcttcac
     1381 tcgtgtcgac tcccagttcc agaagatgta cgctcgccgt gctttcatcc actggtacgt
     1441 caacgaaggc ctcgaaacag tcgaattcga tgaagctcgc tccaacatga cagatctcat
     1501 ccaggagtac gaaatgtacg aaacagccgg
//