HDTenEightyP/NetNews huggingface dataset:
From: Dan_Szymanski@QMS1.LIFE.UIUC.EDU ("Dan Szymanski")
Newsgroups: bionet.molbio.proteins
Subject: stabilizing protein-DNA int
Date: 14 Aug 92 22:57:50 GMT
Distribution: bionet


                       Subject:                               Time:4:55 PM
  OFFICE MEMO          stabilizing protein-DNA interactions   Date:8/14/92
fellow scientists,
i would like some help in choosing a wash buffer for a filter binding assay of
free/bound dna probes.  washing leads to dissociation of the complex and a
deflation of the fraction bound at a given time point.  are there any low ionic
strength solutions which could be used to wash free probe, but favor
association of bound complexes??
Newsgroups: bionet.molbio.proteins
From: adwright@iastate.edu ()
Subject: peptide-bound niacin and biological availability
Keywords: niacin
Organization: Iowa State University, Ames IA
Date: Wed, 12 Aug 1992 14:16:23 GMT

Most of the niacin in cereal grains is bound in biologically unavailable
forms. These include trigonelline, and "niacytin". Another "bound" form
of niacin is peptide-bound.

I am interested in isolation of maize mutants with a higher level of
biologicaly available niacin. I seem to be having some success. I know
that the "niacytin" form (Nic-Aromatic Amine-oligosaccharide) is not
useable by mammals, but I do not know about the peptide-bound form.
Does proteolytic digestion render this form available? I would think so, but
can not find studies to confirm or deny this.
E-mail or posts to this group would be much appreciated.
Allen
From: charley@MCMAIL.CIS.MCMASTER.CA (Calvin Harley)
Newsgroups: bionet.molbio.ageing
Subject: Re: Why does calorie restriction reduce the rate of aging?
Date: 21 Aug 92 20:51:48 GMT
Distribution: bionet

I can't answer Bradbury's general or specific questions, but I would like
to suggest that maybe the way we think about the interaction between
caloric intake and lifespan is backwards. Perhaps it is not that caloric
restriction reduces the rate of aging (or increase maximum longevity), but
rather that ad lib consumption of food increases morbidity and mortality.
I think this is more than a philisophical point. I favour one or more
forms of the antagonistic pleiotropy (Williams' 1957) theory of
senescence, which leads one to predict that many (perhaps thousands) of
genes have co-evolved to dictate a certain maximum genetic lifespan. It
thus is unlikely that a single alteration in lifestyle or diet or a "pill"
will have a dramatic effect on the genetically determined lifespan. Maybe
the lab animals on which the caloric restriction theory has been tested
all have curtailed "normal" lifespans because of ad lib feeding. Maybe
first-world humans are in the same condition. If 120 y is our genetic
maximum, then I would predict that CR would allow more of us to live closer
to 120 y, but it would not push the tail of the curve out to 150-200 y or
more, as some have predicted. None of what I have said undermines the
usefulness of studying CR or ad lib feeding as a model to study factors at
various levels which affect aging. I just think the "experimental system"
is ad lib feeding and so-called caloric restriction may be closer to the
normal "control".

Calvin Harley
Dept. of Biochemistry
McMaster University
Hamilton, ON
Newsgroups: bionet.molbio.ageing
From: lagbok@abo.fi
Subject: Thanks for making the newsgroop alive
Organization: Abo Akademi University, Finland
Date: Sun, 23 Aug 1992 10:10:06 GMT

Thanks for making this newsgroop alive again

I am living in a cold climate here in Finland and people seem to live
to a fairly old age, my grandmother lived over 90 years. She was of the
turtle type of humans though. Also I believe pople living in cool
temperatures are more receptable to viruses like arthtitis (English is 
not my strong side... how is that spelled? - anyway) staying warm
is probably healthier but maybe shortens lifespan? Any comments on that?

In a worm-farm here where they were raising worms for fishing purposes
they found out that worms could live doubble the normal lifespan if
food was given only every seccond day instead of every day like normal.
How was that possible? Is that calorie restriction that is working. Worms
do not have any temperature of their own either.

I think this newsgroop is one of the most important for humans, why are
there so many books written in marketing when the key question of 
surviving here on earth longer gets so little attention. Please get 
discussions going here, it is both interesting and important.

Tom Appelqvist, Box 196, 20101  Turku, Finland (east of Sweden)


Newsgroups: bionet.molbio.ageing
From: dman@netcom.com (Dallman Ross)
Subject: Re: Why does calorie restriction reduce the rate of aging?
Date: Fri, 21 Aug 92 22:54:52 GMT
Organization: Netcom - Online Communication Services  (408 241-9760 guest) 
X-Newsreader: Tin 1.1 PL4

rbradbur@hardy.u.washington.edu (Robert Bradbury) writes:
:                                     When food is scarce, reproduction
: is not a particularly good idea.  Instead an organism would have a
: selective advantage if it could put the resources which would normally
: go into reproduction into preserving the organism until such time as
: times got better and food resources would allow better offspring survival.
: 
So, are you saying that we'll live longer but without any sex drive?
Longer, but less pleasure?


:   2) Protein synthesis is expensive from an energy requirement standpoint.
:      How can an organism get less overall energy and yet increase its
:      protein breakdown and synthesis?  One saves some energy because
:      you do not have the losses associated with building up/breaking
:      down fatty acids.  There also seems to be a less cell replication
:      due to lower growth hormone/T3 levels.  Do these and the slightly
:      lower temperatures provide enough energy savings to be able to
:      increase protein synthesis with 40% less calories or is there
:      something I am missing?
: 
Not sure on that one; but it is clear that less cell replication equates
to less oncogenetic mutation.

Good report.  Thanks.

--
 __D_a l_l m a_n  _ R o_s s _ |dman@netcom.com  /or/  |"You sound like a man|
 l  \\ l\\   /l  /\\  l\\   l |dross@well.sf.ca.us    |with a rubber nose." |
 l   >)l \\ /ll /  \\ l \\  l |vox/fax: 1.510.645.1883| -- One-Lung Bill    |
 l  // l  \X ll/--- \\l  \\ l |350 Perkins St., #108  |     Remmer (deceased)
_l//______________________\\l_|Oakland,_CA__94610-3422|_____________________|_
From: kristoff@genbank.bio.net (David Kristofferson)
Newsgroups: bionet.molbio.ageing
Subject: Re: spelling
Keywords: spelling
Date: 19 Aug 92 17:56:33 GMT
Distribution: bionet
Organization: GenBank Online Service

ipgrier@steam.uwaterloo.ca (Iain P. Grier) writes:

>Do you figure that the person who set up this
>newsgroup is too old to remember how to spell 'aging'?

>Haw haw haw

Haw haw - both spellings are acceptable according to Webster's.

This newsgroup is not "dead" but it doesn't do much more than twitch
occasionally.

				Sincerely,

				Dave Kristofferson
				GenBank Manager

				kristoff@genbank.bio.net
Newsgroups: bionet.molbio.ageing
From: ipgrier@steam.uwaterloo.ca (Iain P. Grier)
Subject: Oops
Keywords: aging, ageing
Organization: University of Waterloo
Distribution: bionet
Date: Wed, 19 Aug 1992 20:44:14 GMT

I guess a good thing about ag(e)ing is that
one gains the maturity to put up with snot-nosed young
punks who don't get their facts straight before posting.

10^3 apologies...
From: ipgrier@steam.uwaterloo.ca (Iain P. Grier)
Subject: spelling
Keywords: spelling
Organization: University of Waterloo
Distribution: bionet
Date: Wed, 19 Aug 1992 15:54:44 GMT

Do you figure that the person who set up this
newsgroup is too old to remember how to spell 'aging'?

Haw haw haw
From: smb3u@kiptron.psyc.virginia.edu (Steven M. Boker)
Newsgroups: bionet.molbio.ageing
Subject: Re: spelling
Keywords: spelling
Date: 19 Aug 92 17:26:28 GMT
Distribution: bionet
Organization: University of Virginia, Department of Psychology

In article <Bt8Mv9.J2B@watserv1.uwaterloo.ca> ipgrier@steam.uwaterloo.ca (Iain P. Grier) writes:
>Do you figure that the person who set up this
>newsgroup is too old to remember how to spell 'aging'?
>

2age vb  aged; ag7ing or age7ing vi
(14c)
1: to become old: show the effects or the characteristics of increasing age
2a: to acquire a desirable quality by standing undisturbed for some time aafter flour is milled it ages PS. C. Prescott & B E. Proctorq
b: to become mellow or mature: RIPEN athis cheese has aged for nearly two yearsq
~ vt
1: to cause to become old
2: to bring to a state fit for use or to maturity 



-- 
 #====#====#====#====#====#====#====#====#====#====#====#====#====#====#====#
 #  Steve Boker           #             "Two's bifurcation                  #
 #  smb3u@virginia.edu    #             but three's chaotic"                #
 #====#====#====#====#====#====#====#====#====#====#====#====#====#====#====#
From: podkayne@AI.MIT.EDU (alice cox)
Newsgroups: bionet.molbio.genome-program
Subject: subscription
Date: 13 Aug 92 19:16:24 GMT
Distribution: bionet


Hi, I'd like to get on this mailing list.
I'm a student at MIT who may major in biology.
I haven't decided yet...

the email address I'd like to receive 
information on is :  "podkayne@athena.mit.edu"

Thanks!
-Alice
Newsgroups: bionet.molbio.genome-program,bionet.genbank
From: francis@monod.Biol.McGill.CA (Francis Ouellette)
Subject: Re: Yeast Chromosome 11 info needed
Organization: McGill University
Date: Thu, 13 Aug 1992 03:27:01 GMT

elliston@msdrl.com (Keith Elliston) writes:

>Hello

>I have a user who would like to get a contig that contains the region on yeast
>chromosome 11, between ura1 and Fas1, or preferably all the way out to mak9. 
>Does anyone know where I can find these data, or who I could ask for help?

well Keith .. a great question for a bionet group I am thinking about
... bionet.molbio.yeast ...  but I will present something about that
when I get back from the international Yeast meeting in Vienna (I go
there tomorrow!).

meanwhile ... CHXI is being "managed" by Bernard Dujon, his Email
address is dujon@pasteur.bitnet ... but I will probably see him before
he sees his email ..  so I will tell him to check it when he gets back.

I am in the progress of getting a "who's who" electronic YEAST email
list together, and I will release it to the net in september ... and I
hope to be able to assign a coordinator for each of the yeast
chromosomes as they appear to all have one at this time.

I also have Dujon's fax and voice mail number somewhere ... I will get
that to you when I get back .       

regards,

francis


--
| B.F. Francis Ouellette  
| manager, yeast chromosome I project
| dept of biology, McGill university, Montreal, Qc, Canada
| francis@monod.biol.mcgill.ca
From: gpersily@lib.ucsf.EDU (Gail Persily)
Newsgroups: bionet.molbio.genome-program
Subject: DNA Symposium
Date: 18 Aug 92 22:33:35 GMT
Distribution: bionet

This announcement is being cross-posted to HUMBIO-L, MEDNEWS, AGRIC-L, 
MEDSTU-L and HUMAN-GENOME-PROGRAM.  I regret any inconvenience this may 
cause.
*       *       *       *       *       *       *       *       *       
	               	WINDING YOUR WAY THROUGH DNA

"Winding Your Way Through DNA" is an exciting adventure in public 
education being held in San Francisco on September 25 and 26, 1992.  The 
day-and-a-half symposium features the world's top scientists and experts 
discussing the history and technology, its applications in medicine and 
agriculture and the ethical and societal ramifications of DNA technology 
-- in terminology we all can understand.  It is being presented by the 
University of California, San Francisco School of Medicine with the San 
Francisco Exploratorium.

Speakers and session hosts include Nobel Laureates James D. Watson, the 
co-discoverer of the double-helix shape of DNA, Paul Berg, Leon Lederman 
and Harold Varmus; Herbert W. Boyer and  Stanley N. Cophen, whose 
innovative DNA cloning led to the birth of genetic engineering; Eric 
Lander, an expert on the use of DNA in forensics; Donald Kennedy who 
will address issues of regulation; and many more speakers.

Because of the overwhelming interest expressed by hospital and 
biotechnology industry employees, high school and college students and 
teachers and a very interested public, the symposium is now available 
for viewing live via satellite transmission.  If your facility is 
interested in learning more about receiving the symposium, please 
contact Katherine Riordan at 415/476-6714, or by FAX at 415/476-4099.

If you are interested in purchasing a set of videotapes of the 
symposium, please contact Ms. Riordan at the above numbers.

*       *       *       *       *       *       *       *       *  
Please feel free to forward this announcement to any groups or 
individuals you feel may be interested.

Do not respond to sender, but to individual named at end of 
announcement.  Thank you.
Newsgroups: bionet.molbio.genome-program,bionet.genbank
From: elliston@msdrl.com (Keith Elliston)
Subject: Yeast Chromosome 11 info needed
Organization: Merck Research Laboratories
Date: Wed, 12 Aug 92 19:08:59 GMT

Hello

I have a user who would like to get a contig that contains the region on yeast
chromosome 11, between ura1 and Fas1, or preferably all the way out to mak9. 
Does anyone know where I can find these data, or who I could ask for help?

Thanks

Keith Elliston
elliston@msdrl.com
-- 
Keith O. Elliston            elliston@msdrl.com           uunet!av8tr!elliston
AA5A/U  N9734U             elliston@mbcl.rutgers.edu     elliston@biovax.bitnet
"Beware of pseudo-experts with a mission and a grudge, especially if they are
 lawyers pretending to be scientists." -- H.W. Lewis in 'Technological Risk'
From: bsp1sz@surrey.ac.uk (ZHANG S MR)
Newsgroups: bionet.jobs
Subject: subscription to employment
Date: 19 Aug 92 10:25:25 GMT
Distribution: bionet

Subscription to employment.
From: una@phy.duke.edu (Una Smith)
Newsgroups: bionet.jobs
Subject: Ecology graduate assistantship
Date: 18 Aug 92 19:57:06 GMT
Organization: Duke University Physics Dept.; Durham, N.C.


Forwarded from OTS-L


A graduate research associate position is available for 3 years beginning
September 1993 in the Department of Ecology and Evolutionary Biology,
University of Connecticut, Storrs, CT.    This student will participate in an
NSF-funded project on environmental heterogeneity and woody species
diversity in low-elevation secondary forests of northeastern Costa Rica.
The position requires spending approximately 6 months a year in Costa Rica,
based at La Selva Biological Station (Organization for Tropical Studies).
Knowledge of Spanish and field research experience in plant ecology,
tropical  biology, forest ecology, micrometeorology, or plant physiological
ecology is strongly recommended.  A B.A. or B.S. degree is required.  The
student will work closely with project co-P.I.'s Dr. Robin Chazdon and Dr.
Julie Denslow (Tulane University), and is expected to conduct research
leading to the completion of a Ph.D. degree in Ecology and Evolutionary
Biology.  Specific duties of the research assistant may include studies of
growth and ecophysiology of transplanted tree seedlings and shrub cuttings,
measurements of forest structure, light environments and canopy
characteristics, and assistance with the development of a mechanistic,
simulation model of tropical forest dynamics.  For further information and
graduate school application materials please contact:  Dr. Robin Chazdon,
Department of Ecology and Evolutionary Biology, University of Connecticut,
Storrs, CT  06268-3042, e-mail address Chazdon@UCONNVM.bitnet, voice phone (203)
486-4057, or FAX (203) 486-6364.


End of forwarded message.  Please do not reply to me.
-- 

Una Smith   una@phy.duke.edu    School of the Environment
                                Duke University
                                Durham, NC  27706
Newsgroups: bionet.jobs
From: lboulet@magnus.acs.ohio-state.edu (Lillace D Boulet)
Subject: how to subscribe to cin.jobs?
Organization: The Ohio State University
Distribution: oh
Date: Wed, 19 Aug 1992 15:46:29 GMT

recently i read thru a bunch of postings stating that cincinnati and cleveland 
had similar newsgroups as columbus....i.e.  cmh.forsale = cin.forsale.  what i 
am interested in is a jobs newsgroup from cincinnati.  someone posted that 
there was such a newsgroup....is there?  and how does one suscribe?

i have tried to suscribe through the net at osu (g cin.jobs) but i received a
message that the newsgroup does not exist.  i would appricate any comments or 
suggestions on this matter.

boulet.1@osu.edu
From: lpan@uk.ac.crc (Dr. Langxing Pan)
Newsgroups: bionet.jobs
Subject: application
Date: 21 Aug 92 17:26:38 GMT
Distribution: bionet
Original-To: biojobs@uk.ac.daresbury

Can I join the list?

Dr. Pan LX
Histopathology
UCH
London
From: GWU5042@GWUVM.GWU.EDU
Newsgroups: bionet.jobs
Subject: looking for science writing position
Date: 18 Aug 92 15:42:11 GMT
Distribution: bionet

Medical science writer/editor with master's degree in biochemistry
is looking for research/writing position in Washington, DC area.  Has 4 years
experience writing proposals for biotechnology company, 2 years experience
writing for the National Institutes of Health, and 3 years writing for
pharmaceutical companies.
Please contact Patricia Duncan, 4808 Moorland Lane, #903, Bethesda, MD
20814, (301) 654-3918.
 or email : gwu5042@gwuvm.gwu.edu
From: pxlin@HELIX.NIH.GOV
Newsgroups: bionet.jobs
Subject: suscribe
Date: 21 Aug 92 16:07:35 GMT
Distribution: bionet

please send mail about information to me
Dr. Lin Peng Xin
Bldg.36 Rm2A21
NIH
Bethesda, MD 20892
Tel (301) 402-1484
Newsgroups: uiuc.jobs.offered,uiuc.cs.jobs,bionet.jobs
From: mrmike@geta.life.uiuc.edu (Mike McCaughey)
Subject: Research Information Specialist - Urbana,IL
Organization: University of Illinois at Urbana
Date: Wed, 12 Aug 1992 18:35:04 GMT



                RESEARCH INFORMATION SPECIALIST

   The Department of Microbiology, University of Illinois at
Urbana-Champaign, seeks a qualified Research Information Specialist to
assist in the development and maintenance of a ribosomal RNA sequence
database.  The primary duty will be to act as curator for the database.
These duties will include keeping the database current, coordinating
data exchange with other molecular biology databases, coordinating
activities necessary to assure availability and distribution of data
collection to its users, and collaborating with biologists and
computer scientists on the development of new software tools for
the maintenance and analysis of the data collection.  This is a
full time position.  The candidate should have a bachelor's or
master's degree in computer science or in library science
(with emphasis on databasing), or a bachelor's degree or higher
in biology (with extensive training in computers/databasing).

   Salary is negotiable on the basis of experience and qualifications.
Anticipated starting date is September 15, 1992.  This position is
supported by a grant from the National Science Foundation, and
continuation of the position is contingent upon continuation of grant funds.

   Applicants should send a resume, a summary of relevant
experience, and the names and addresses (incl. electronic)
of three references to: Ms. Carolyn Corn, Administrative
Assistant, Department of Microbiology, University of Illinois,
131 Burrill Hall, 407 S. Goodwin Avenue, Urbana, IL 61801;
Phone: 217-333-1736.

   To receive full consideration, applications should be received
by September 1, 1992.  Interviews may be conducted as soon as
applications are received.  However, all applications that are
received by the above date will be given equal consideration.


*The University of Illinois is an Equal Opportunity/Affirmative
Action Employer.
From: dokeefe@edu.ufl.agen.water (David Okeefe)
Newsgroups: bionet.jobs
Subject: bionet.jobs mail newsgroup
Date: 21 Aug 92 20:43:02 GMT
Distribution: bionet
Original-To: biojobs@uk.ac.daresbury

Please help me get off this list.
From: vanrooij@ACS.UCALGARY.CA
Newsgroups: bionet.jobs
Subject: job
Date: 21 Aug 92 21:54:20 GMT
Distribution: bionet


I received my Ph.D. degree in molecular biology, working on Neurospora (common 
bread mold). My area of specialization is heat-shock gene expression. 
I am seeking a post-Doctoral position in a lab that is currently working
on stress-induced gene expression either in plants or in animal 
systems. Please feel free to contact me by phone (403)220-7907 (Lab), (403)
286-0512 (res). I will send my CV if requested. Please use the following 
address for mailing: 
                   Dr. Hiranya S. Roychowdhury, Dept of Biological Sciences,
			University of Calgary, Calgary T2N 1N4, Alberta,
				Canada.

					OR:
		   Dr. Hiranya S. Roychowdhury,
		   B102, 4501-37th. Street, N.W.
	           Calgary T2L 2J5, Alberta, Canada.

I am also interested in joining the industrial sector.
From: skspoidn@uk.ac.rdg.susssys1 ("Mike Poidinger")
Newsgroups: bionet.jobs
Subject: NIH post-Docs
Date: 3 Aug 92 19:16:58 GMT
Distribution: bionet
Original-To: biojobs@uk.ac.daresbury

Sorry for yet more of the same, but I just got a
 reply to my earlier post from Jim (?), the mail
 now stupidly deleted, which said that the NIH Email
 address was cu.nih.gov. My question is who at this 
address? ie **@cu.nih.gov.

Thanks,
Mike Poidinger
Dept of Microbiology
University of Reading
skspoidn@susssys1.rdg.ac.uk
From: hatamian@gandalf.rutgers.edu (ShAhin)
Newsgroups: bionet.jobs
Subject: MS-Biomed Seeking Employment
Date: 19 Aug 92 15:08:37 GMT
Distribution: usa
Organization: Rutgers Univ., New Brunswick, N.J.



Hello,

A friend has asked me to post this for her. Please respond directly to her
or via email to me for a complete resume.
____________________________________________________________________________

Hi,

I am graduating in December '92 from NJIT with a MS in Biomedical Engineering.
I am currently employed by a well-known hospital products manufacturing
company as a Product Development Engineer. I am conducting my thesis research
concurrently at this company.
please feel free to contact me for a resume and/or to discuss my qualifications
further.

Thank you for your time.

Please call Elli at (201)644-9174

____________________________________________________________________________
Newsgroups: bionet.plants
From: delwiche@sunflower.bio.indiana.edu (Charles Delwiche)
Subject: Re: [Beverly Erlebacher: Re: energy crops] "Canola" oil
Organization: Biology, Indiana University - Bloomington
Distribution: bionet
Date: Sat, 22 Aug 1992 21:21:02 GMT

On 22 Aug 92 00:14:01 GMT Beverly Erlebacher wrote:

> p.s. Rapeseed is also called colza.  The name "canola" is a recent
>neologism, coined because people are uncomfortable saying "rape" or
>"rapeseed".  I assume it derives from "Canadian" and "oil"

"Canola", so far as I understand it, was coined from "canadian low
erucic acid rapeseed oil".  Erucic acid is a nasty tasting and
probably toxic fatty acid which is present in high concentrations in
older varieties of rapeseed (= colza/canola), which was virtually
eliminated from some cultivars through a breeding program.  This gave
rise to the current market for canola as a food oil.  Interestingly
enough, erucic acid, although undesireable in food, is one of the
reasons that rapeseed oil is an important industrial oil for
lubrication and quenching.  Apparently erucic acid helps the oil
withstand high temperatures without turning into tar.  I don't know if
erucic acid content would affect its value as a fuel oil.

-----

Charles Delwiche
-- 
----------------------------------------------------------------------------
Charles F. Delwiche, Dept. of Biology        |"We admit no more causes...
Indiana University, Bloomington, IN 47405    |than such as are both true
                                             |and sufficient." I. Newton