From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Arachis hypogea resveratrol synthase gene, complete cds.
Date: 1 Sep 92 10:00:46 GMT
Distribution: bionet
Checksum: 32230     5

LOCUS       ARQRES01     1170 bp ds-DNA             PLN       01-SEP-1992
DEFINITION  Arachis hypogea resveratrol synthase gene, complete cds.
ACCESSION   L00952 L00953 L00954
KEYWORDS    resveratrol synthase; stilbene synthase.
SOURCE      Arachis hypogea (library: lambda NM1149) DNA.
  ORGANISM  Arachis hypogea
            Eukaryota; Plantae; Embryobionta; Magnoliophyta; Magnoliopsida;
            Rosidae; Fabales; Fabaceae.
REFERENCE   1  (bases 1 to 1170)
  AUTHORS   Lanz,T., Schroeder,G. and Schroeder,J.
  TITLE     Differential regulation of genes for resveratrol synthase in cell
            cultures of Arachis hypogaea L
  JOURNAL   Planta 181, 169-175 (1990)
  STANDARD  full staff_review
REFERENCE   2  (bases 1 to 1170)
  AUTHORS   Schroeder,J.
  TITLE     The role of cysteines in polyketide synthases. Site-directed
            mutagenesis of resveratrol and chalcone synthases, two key enzymes
            in different plant specific pathways
  JOURNAL   J. Biol. Chem. 266, 9971-9976 (1991)
  STANDARD  full staff_review
REFERENCE   3  (sites)
  AUTHORS   Schroeder,G. and Schroeder,J.
  TITLE     A single change of histidine to glutamine alters the substrate
            preference of a stilbene synthase
  JOURNAL   J. Biol. Chem. (1992) In press
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     CDS             1..1170
                     /EC_number="2.3.1."
                     /standard_name="stilbene synthase"
                     /note="enzyme is functional after protein expression in
                     Escherichia coli"
                     /product="resveratrol synthase"
                     /evidence=EXPERIMENTAL
                     /codon_start=1
                     /translation="MVSVSGIRKVQRAEGPATVLAIGTANPPNCIDQSTYADYYFRVT
                     NSEHMTDLKKKFQRICERTQIKNRHMYLTEEILKENPNMCAYKAPSLDAREDMMIREV
                     PRVGKEAATKAIKEWGQPMSKITHLIFCTTSGVALPGVDYELIVLLGLDPCVKRYMMY
                     HQGCFAGGTVLRLAKDLAENNKDARVLIVCSENTAVTFRGPSETDMDSLVGQALFADG
                     AAAIIIGSDPVPEVEKPIFELVSTDQKLVPGSHGAIGGLLREVGLTFYLNKSVPDIIS
                     QNINDALNKAFDPLGISDYNSIFWIAHPGGRAILDQVEQKVNLKPEKMKATRDVLSNY
                     GNMSSACVFFIMDLMRKRSLEEGLKTTGEGLDWGVLFGFGPGLTIETVVLRSVAI"
                     /citation=[1]
                     /citation=[2]
     mutation        481..486
                     /phenotype="mutant"
                     /note="mutation of HisGln to GlnHis close to active site
                     cysteine inactivates enzyme"
                     /evidence=EXPERIMENTAL
     mutation        483
                     /phenotype="mutant"
                     /note="the mutation from HisGln to GlnGln close to the
                     active site cysteine changes the substrate preference of
                     the enzyme"
                     /evidence=EXPERIMENTAL
                     /citation=[3]
BASE COUNT      328 a    229 c    293 g    320 t
ORIGIN
        1 atggtgtctg tgagtggaat tcgcaaggtt caaagggcag aaggtccagc aactgtattg
       61 gcaattggaa cagcaaatcc accgaactgt attgatcaga gtacatatgc agattattat
      121 tttagagtaa ccaatagcga acacatgact gatctcaaga agaaatttca gcgcatctgt
      181 gagagaacac agatcaagaa cagacatatg tacttaacag aagagatact aaaagaaaat
      241 cctaacatgt gtgcatacaa ggcaccgtca ttggatgcaa gagaagacat gatgatcagg
      301 gaggtaccaa gagttggaaa agaggctgca accaaggcca tcaaggaatg gggccagcca
      361 atgtctaaga tcacacattt gatcttctgc accaccagcg gcgttgcgtt gcctggcgtt
      421 gattacgaac tcatcgtact tttagggctg gacccatgcg tcaagaggta catgatgtac
      481 caccaaggtt gcttcgctgg tggcactgtc cttcgtttgg ctaaggactt ggctgaaaac
      541 aacaaggatg ctcgtgtact tatcgtttgt tctgagaata ccgcagtcac tttccgcggt
      601 cctagtgaga cagacatgga tagtcttgta ggacaagcat tgtttgccga tggagctgct
      661 gcgattatca ttggttctga tcctgtgcca gaggttgaga agcctatctt tgagcttgtt
      721 tcgaccgatc aaaaacttgt ccctggcagc catggagcca tcggtggtct ccttcgtgaa
      781 gttggactta cattctatct taacaagagt gttcctgata ttatttcgca aaatatcaat
      841 gacgcgctca ataaagcttt tgatccattg ggtatttctg attataactc aatattttgg
      901 attgcacatc ctggtgggcg tgcaattttg gaccaggttg aacagaaggt gaacttgaag
      961 ccagagaaga tgaaagccac tagagatgtg cttagcaatt atggtaacat gtcaagtgcc
     1021 tgtgtgttct tcattatgga tttgatgagg aagaggtctc ttgaagaagg acttaaaact
     1081 accggagaag gacttgattg gggtgtgctt tttggctttg gtcctggtct cactattgaa
     1141 actgtcgttc tccgcagtgt ggccatataa
//