From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Transposon Tn2426 (from Shigella sonnei) integrase (tnpI),
Date: 27 Jan 93 06:02:40 GMT
Distribution: bionet
Checksum: 43298 10

LOCUS       TRNTN21CAS   3505 bp ds-DNA             BCT       26-JAN-1993
DEFINITION  Transposon Tn2426 (from Shigella sonnei) integrase (tnpI),
            2'-O-adenyltransferase (aadB), 3'-(9)-O-adenyltransferase (aadA),
            and 6'-N-acetyltransferase (aacA) genes, complete cds's.
ACCESSION   M86913
KEYWORDS    2'-O-adenyltransferase; 3'-(9)-O-adenyltransferase;
            6'-N-acetyltransferase; Tn21-cassette; Tn2426 transposable element;
            integrase.
SOURCE      Transposon Tn2426 DNA.
  ORGANISM  Transposon Tn2426
            Prokaryotae.
REFERENCE   1  (sites)
  AUTHORS   Fling,M.E., Kopf,J. and Richards,C.
  TITLE     Nucleotide sequence of the transposon Tn7 gene encoding an
            aminoglycoside-modifying enzyme, 3'(9)-O-nucleotidyltransferase
  JOURNAL   Nucleic Acids Res. 13, 7095-7106 (1985)
  STANDARD  full automatic
REFERENCE   2  (sites)
  AUTHORS   Wiedemann,B.
  JOURNAL   Antimicrob. Agents Chemother. 18 Su, 85-92 (1986)
  STANDARD  full automatic
REFERENCE   3  (sites)
  AUTHORS   Cameron,F.H., Obbink,D.J., Ackerman,V.P. and Hall,R.M.
  TITLE     Nucleotide sequence of the AAD(2'') aminoglycoside
            adenylyltransferase determinant aadB. Evolutionary relationship of
            this region with those surrounding aadA in R538-1 and dhfrII in
            R388
  JOURNAL   Nucleic Acids Res. 14, 8625-8635 (1986)
  STANDARD  full automatic
REFERENCE   4  (sites)
  AUTHORS   Tenover,F.C., Filpula,D., Phillips,K.L. and Plorde,J.J.
  TITLE     Cloning and sequencing of a gene encoding an aminoglycoside
            6'-N-acetyltransferase from an R factor of Citrobacter diversus
  JOURNAL   J. Bacteriol. 170, 471-473 (1988)
  STANDARD  full automatic
REFERENCE   5  (sites)
  AUTHORS   Mercier,J., Lachapelle,J., Couture,F., Lafond,M., Vezina,G.,
            Boissinot,M. and Levesque,R.C.
  TITLE     Structural and functional characterization of tnpI a recombinase
            locus in Tn21 and related transposons
  JOURNAL   J. Bacteriol. 172, 3745-3757 (1990)
  STANDARD  full automatic
REFERENCE   6  (bases 1 to 3505)
  AUTHORS   Zuehlsdorf,M.T. and Wiedemann,B.
  TITLE     Functional and physiological characterization of the Tn21-cassette
            for resistance genes in Tn2426
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     -10_signal      complement(1179..1184)
                     /gene="tnpI"
                     /note="promoter Pint"
     -10_signal      1180..1185
                     /note="promoter Pout for aadA, aadB and aacA"
     -35_signal      complement(1203..1208)
                     /gene="tnpI"
                     /note="promoter Pint"
     repeat_unit     1279..1292
                     /note="recombination sequence (rs)"
     repeat_unit     1829..1880
                     /note="recombination sequence (rs)"
     repeat_unit     2683..2737
                     /note="recombination sequence (rs)"
     repeat_unit     3330..3342
                     /note="recombination sequence (rs)"
     CDS             complement(137..1150)
                     /gene="tnpI"
                     /product="integrase"
                     /codon_start=1
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYFHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     CDS             1299..1832
                     /gene="aadB"
                     /note="inserted into the backbone of Transposon Tn21 at
                     recombination sequences (rs)"
                     /product="2'-O-adenyltransferase"
                     /codon_start=1
                     /translation="MDTTQVTLIHKILAAADERNLPLWIGGGWAIDARLGRVTRKHDD
                     IDLTFPGERRGELEAIVEMLGGRVMEELDYGFLAEIGDELLDCEPAWWADEAYEIAEA
                     PQGSCPEAAEGVIAGRPVRCNSWEAIIWDYFYYADEVPPVDWPTKHIESYRLACTSLG
                     AEKVEVLRAAFRSRYAA"
     CDS             1888..2679
                     /gene="aadA"
                     /product="3'-(9)-O-adenyltransferase"
                     /codon_start=1
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     CDS             2775..3332
                     /gene="aacA"
                     /note="inserted into the backbone of Transposon Tn21 at
                     recombination sequences (rs)"
                     /product="6'-N-acetyltransferase"
                     /codon_start=1
                     /translation="MNYQIVNIAECSNYQLEAANILTEAFNDLGNNSWPDMTSATKEV
                     KECIESPNLCFGLLINNSLVGWIGLRPMYKETWELHPLVVRPDYQNKGIGKILLKELE
                     NRAREQGIIGIALGTDDEYYRTSLSLITITEDNIFDSIKNIKNINKHPYEFYQKNGYY
                     IVGIIPNANGKNKPDIWMWKSLIKE"
BASE COUNT      917 a    849 c    978 g    761 t
ORIGIN      
        1 ggatccatca ggcaacgacg ggctgctgcc ggccatcagc ggacgcaggg aggactttcc
       61 gcaaccggcc gttcgatgcg gcaccgatgg ccttcgcgca ggggtagtga atccgccagg
      121 attgacttgc gctgccctac ctctcactag tgaggggcgg cagcgcatca agcggtgagc
      181 gcactccggc accgccaact ttcagcacat gcgtgtaaat catcgtcgta gagacgtcgg
      241 aatggccgag cagatcctgc acggttcgaa tgtcgtaacc gctgcggagc aaggccgtcg
      301 cgaacgagtg gcggagggtg tgcggtgtgg cgggcttcgt gatgcctgct tgttctacgg
      361 cacgtttgaa ggcgcgctga aaggtctggt catacatgtg atggcgacgc acgacaccgc
      421 tccgtggatc ggtcgaatgc gtgtgctgcg caaaaaccca gaaccacggc caggaatgcc
      481 cggcgcgcgg atacttccgc tcaagggcgt cgggaagcgc aacgccgctg cggccctcgg
      541 cctggtcctt cagccaccat gcccgtgcac gcgacagctg ctcgcgcagg ctgggtgcca
      601 agctctcggg taacatcaag gcccgatcct tggagccctt gccctcccgc acgatgatcg
      661 tgccgtgatc gaaatccaga tccttgaccc gcagttgcaa accctcactg atccgcatgc
      721 ccgttccata cagaagctgg gcgaacaaac gatgctcgcc ttccagaaaa ccgaggatgc
      781 gaaccacttc atccggggtc agcaccaccg gcaagcgccg cgacggccga ggtcttccga
      841 tctcctgaag ccagggcaga tccgtgcaca gcaccttgcc gtagaagaac agcaaggccg
      901 ccaatgcctg acgatgcgtg gagaccgaaa ccttgcgctc gttcgccagc caggacagaa
      961 atgcctcgac ttcgctgctg cccaaggttg ccgggtgacg cacaccgtgg aaacggatga
     1021 aggcacgaac ccagtggaca taagcctgtt cggttcgtaa gctgtaatgg aagtagcgta
     1081 tgcgctcacg caactggtcc agaaccttga ccgaacgcag cggtggtaac ggcgcagtgg
     1141 cggttttcat ggcttgttat gactgttttt ttggggtaca gtctatgcct cgggcatcca
     1201 agcagcaagc gcgttacgcc gtgggtcgat gtttgatgtt atggagcagc aacgatgtta
     1261 cgcagcaggg cagtcgccct aaaacaaagt taggccgcat ggacacaacg caggtcacat
     1321 tgatacacaa aattctagct gcggcagatg agcgaaatct gccgctctgg atcggtgggg
     1381 gctgggcgat cgatgcacgg ctagggcgtg taacacgcaa gcacgatgat attgatctga
     1441 cgtttcccgg cgagaggcgc ggcgagctcg aggcaatagt tgaaatgctc ggcgggcgcg
     1501 tcatggagga gttggactat ggattcttag cggagatcgg ggatgagtta cttgactgcg
     1561 aacctgcttg gtgggcagac gaagcgtatg aaatcgcgga ggctccgcag ggctcgtgcc
     1621 cagaggcggc tgagggcgtc atcgccgggc ggccagtccg ttgtaacagc tgggaggcga
     1681 tcatctggga ttacttttac tatgccgatg aagtaccacc agtggactgg cctacaaagc
     1741 acatagagtc ctacaggctc gcatgcacct cactcggggc ggaaaaggtt gaggtcttgc
     1801 gtgccgcttt caggtcgcga tatgcggcct aacaattcgt ccaagccgac gccgcttcgg
     1861 cgcggcttaa ctcaggtgtt aaacatcatg agggaagcgg tgatcgccga agtatcgact
     1921 caactatcag aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt gctggccgta
     1981 catttgtacg gctccgcagt ggatggcggc ctgaagccac acagtgatat tgatttgctg
     2041 gttacggtga ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg
     2101 gaaacttcgg cttcccctgg agagagcgag attctccgcg ctgtagaagt caccattgtt
     2161 gtgcacgacg acatcattcc gtggcgttat ccagctaagc gcgaactgca atttggagaa
     2221 tggcagcgca atgacattct tgcaggtatc ttcgagccag ccacgatcga cattgatctg
     2281 gctatcttgc tgacaaaagc aagagaacat agcgttgcct tggtaggtcc agcggcggag
     2341 gaactctttg atccggttcc tgaacaggat ctatttgagg cgctaaatga aaccttaacg
     2401 ctatggaact cgccgcccga ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc
     2461 cgcatttggt acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg
     2521 gcaatggagc gcctgccggc ccagtatcag cccgtcatac ttgaagctag acaggcttat
     2581 cttggacaag aagaagatcg cttggcctcg cgcgcagatc agttggaaga atttgttcac
     2641 tacgtgaaag gcgagatcac caaggtagtc ggcaaataat gtctaacaat tcgttcaagc
     2701 cgacgccgct tcgccggctt aactcaagcg ttagggcgac gccgctattg cggcgcgaat
     2761 acaaagagga agagatgaat tatcaaattg tgaatattgc ggaatgcagc aattatcagt
     2821 tagaagcagc aaatatacta acagaagcgt tcaatgatct tggtaacaat tcatggccag
     2881 atatgacgag tgcaacaaaa gaagtaaaag aatgtattga gagtccaaac ctttgtttcg
     2941 gtctgctaat aaataactcc ttagttggct ggataggctt aaggccaatg tacaaggaaa
     3001 cgtgggaatt gcatccattg gttgtcagac cagattatca aaataaaggt attggcaaga
     3061 tcctgcttaa ggaattagaa aacagagcta gagagcaagg tattattgga atcgctttag
     3121 gaacagatga tgaatactat agaacaagtc tctctttaat aactataaca gaagataata
     3181 tatttgattc aataaaaaat attaaaaata ttaataaaca tccatatgag ttttatcaga
     3241 agaatggtta ttatattgtt ggaataattc caaatgccaa tggtaaaaac aaaccagata
     3301 tttggatgtg gaaaagttta atcaaagagt aaaacaatgg aacagattaa taacatagaa
     3361 cttgtagatc catctatcta cccaacagat gaaatcttaa aaagaatact tggaaaatcg
     3421 tttagtatat atttaaaatt actacgactc tatgaaaatt atagtttaat accagagtgg
     3481 aagtattata aagatggaaa agctt
//