From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Drosophila melanogaster P-glycoprotein/multidrug resistance protein
Date: 22 Dec 92 22:51:03 GMT
Distribution: bionet
Checksum: 01656 12

LOCUS       DROMDR50A    5348 bp ds-DNA             INV       30-NOV-1992
DEFINITION  Drosophila melanogaster P-glycoprotein/multidrug resistance protein
            (Mdr50) gene, complete cds.
ACCESSION   L07065
KEYWORDS    P-glycoprotein; multidrug resistance protein.
SOURCE      Drosophila melanogaster (strain Canton S) (library: Stratagene
            Sau3A into XhoI, FIX II vector) embryo DNA.
  ORGANISM  Drosophila melanogaster
            Eukaryota; Animalia; Metazoa; Arthropoda; Uniramia; Insecta;
            Pterygota; Neoptera; Holometabola; Diptera; Brachycera;
            Cyclorrhapha; Schizophora; Drosophiloidea; Drosophilidae.
REFERENCE   1  (bases 1 to 5348)
  AUTHORS   Gerrard,B.C., Stewart,C. and Dean,M.
  TITLE     Analysis of Mdr50: A Drosophila P-glycoprotein/multidrug resistance
            gene
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     exon            <823..1083
                     /number=1
                     /gene="Mdr50"
     intron          1084..1152
                     /number=1
                     /gene="Mdr50"
     exon            1153..1436
                     /number=2
                     /gene="Mdr50"
     intron          1437..1615
                     /number=2
                     /gene="Mdr50"
     exon            1616..3565
                     /number=3
                     /gene="Mdr50"
     intron          3566..3624
                     /number=3
                     /gene="Mdr50"
     exon            3625..3931
                     /number=4
                     /gene="Mdr50"
     intron          3932..3994
                     /number=4
                     /gene="Mdr50"
     exon            3995..4134
                     /number=5
                     /gene="Mdr50"
     intron          4135..4203
                     /number=5
                     /gene="Mdr50"
     exon            4204..4765
                     /number=6
                     /gene="Mdr50"
     intron          4766..4827
                     /number=6
                     /gene="Mdr50"
     exon            4828..4974
                     /number=7
                     /gene="Mdr50"
     intron          4975..5037
                     /number=7
                     /gene="Mdr50"
     exon            5038..>5238
                     /number=8
                     /gene="Mdr50"
     CDS             join(823..1083,1153..1436,1616..3565,3625..3931,
                     3995..4134,4204..4765,4828..4974,5038..5238)
                     /gene="Mdr50"
                     /product="P-glycoprotein/multidrug resistance protein"
                     /codon_start=1
                     /translation="MIGVRTLYYYCSEPNKSKKKSKHDESDASDEEDGSEYHEDVKQV
                     SYFQLFRYATKKDRALYVIGLLSAVGTGLTTPANSLIFGNLANDMIDLSGLLESGKSY
                     RADDAISTLLLDKVRQFSLQNTYIGMIMLVCSYLSITCFNYAAHSQILTIRSKFFRSI
                     LHQDMKWYDFNQSGEVASRMNEDLSKMEDGLAEKVVMFVHYLVAFVGSLVLAFVKGWQ
                     LSLVCLTSLPLTFIAMGLVAVATSRLAKKEVTMYAGAAVVAEGALSGIRTVKAFEGEA
                     KEVAAYKERVVAAKILNIKRNMFSGIGFGLLWFFFIYASYALAFWYGVGLVIESAYEN
                     YDAGTMITVFFSVMMGSMNIGMAAPYIEAFGIAQGRLPKVFHIIEQIPEINPLMGRVK
                     VNEPLTTIEFKEVEFQYPTRPEVSILNKLNLKIHRGQTVALVGPSGCGKSTCIQLVQR
                     FYDPQAGNLLFNGTNLKDLDINWLRFLRIGVVGQEPILFATSIYENIRYGREDATREE
                     IEAARAAANAAIFIKKLPKGYDTLVGERGAQLSGGQKQRIAIRRALIRDPEILLLDEA
                     TSALDTASEAKVQAALEKVSAGRTTIIVAHRLSTVRRADRIVVINKGEVVESGTHQEL
                     MELKDHYFNLVTTQLGEDDGSVLSPTGDIYKNFDIKDEDEEEIKVLSEDEDEDVMVTD
                     EKNKKKKMKKVKDPNEVKPMLEVMKMNKPEWLQIAVGCISSVIMGCAMPIFAVLFGSI
                     LQILSVKDNDQYVRENSNQYSLYFLIAGIVVGIATFLQIYFFGIAGERLTERLRGLMF
                     EAMLRQEVAWFDDKANGTGSLCARLSGDAAAVQGATGQRIGTIIQSISTLALGIALSM
                     YYEWSLGLVALAFTPFILIAFYMQRTLMAKENMRPAKTMENCTKLAVEVVSNIRTVAS
                     LGREEMFHQNYIGMLIPAVEISKRNTHFRGLVYGLARSLMFFAYAACMYYGTWCVIHR
                     GILFGDVFKVSQAVIMGTASIANALAFAPNMQKGVSAAKTIFTFLRRQPSIVDRPGVS
                     RDPWHSEGYVRFDKVKFSYPTRSEIQVLKGLELAVSKGQKIALVGPSGCGKSTCIQLI
                     QRFYDVDEGATLIDECDVRNVSMTNLRNQLGIVSQEPILFDRTIRENISYGDNARNVT
                     DQEIISACKKSNIHEFIANLPLGYDTRMGEKGAQLSGGQKQRIAIARALIRNPKIMLL
                     DEATSALDAESEKVVQDALDAASEGRTTISIAHRLSTVVHSDVIFVFENGLVCEAGDH
                     KQLLANRGLYYTLYKLQSGSM"
BASE COUNT     1415 a   1205 c   1285 g   1443 t
ORIGIN      
        1 gaattcgcga tcgcagtaca aaacgaacat cgtcttgggc gccaaactag aggattcgga
       61 tcgagatcga aagagcttgt gagttttgaa aagaaagtcg ttcaaaggac attcaaagtt
      121 tgaaagcgga aatcggaatt ttgtgctgtg ccaacgaaat ggctgtaatc tcaattaatc
      181 ctgttaatac ctattgtcct ataggtatta aatcaatcga tactcaaatg aataaccgga
      241 agaaattatt ttattaattg acgtgtttcc gtcgagtgcc caattccatt caagttgttc
      301 acctttcaca aatttgcact tcaacgatga cgccgctgaa ttttcatacg cattattcag
      361 ctactaagtg gtaattttaa ttgtgtaccc atatgcggaa gaagtctaat aacggaacat
      421 atcacataat ttgcaaggat ctttaaatta aagtttgagc ctgaaatcaa tgctttacaa
      481 agctctacag ttttgtaacc cacataaaag attatatatg tagattgtgg agcgaacttt
      541 cgaaagttag caaatagtgt aaagtgtatt tggccttcgt tataatattc gaacaattac
      601 aactttatcg ttcattgatt gccaatgagt taaaccaaaa aaaaaatata gatagtaagt
      661 tcgtcgggca aaaccgaagc gtggtcattg tgttattggt tgttgcaaac cacttctcac
      721 ggagaatgtt aggtacagat aagcttggcg cgattggtga ttgacagcac tgtggggttc
      781 atggcaatgg cgtcaataga atcatcgaac tgataaccat ctatgattgg cgtgcgcacc
      841 ctttattatt attgcagtga gcccaacaag tcgaaaaaga agtccaagca tgacgagtcg
      901 gatgccagcg atgaggagga tggctccgag taccatgagg atgtcaagca ggttagctac
      961 ttccagctct ttcggtacgc caccaaaaag gatcgagcgc tctatgtgat tggactactg
     1021 tctgccgtcg gcacaggcct gaccactccg gccaacagtt tgatattcgg taatctcgcc
     1081 aatgtaagtc aaaaatgctg ttccaaaatc atagatcttt atctatttat acatctatct
     1141 taatatccaa aggacatgat tgacttgagt ggactgcttg agagcggaaa atcgtatcga
     1201 gctgacgatg ctatttctac tttgcttctg gacaaagtgc ggcagttctc cctgcaaaac
     1261 acctacatcg gcatgattat gctggtgtgt tcctatctct cgatcacctg cttcaactac
     1321 gcggcccact cccagatcct caccatccgc tcaaagttct ttcgctccat tctgcatcag
     1381 gacatgaagt ggtacgactt caaccagagt ggcgaagtag ccagccgaat gaatgagtaa
     1441 gtgactcaca catcttatca aatctttagt taatcaactg gaatttttgt catacgtaac
     1501 catacaccaa acaagtcaca cgtttcttat cagttgcttt caaagttacc atcttataaa
     1561 tagttaaaaa aaatcaaaga tatgccaatc gaattaaatg tgccgccttt ttcagagatc
     1621 tctccaaaat ggaggatggc ttggccgaaa aagtggtcat gttcgtccat taccttgtcg
     1681 ccttcgtggg atctttggtg ctagcctttg tcaagggctg gcagctatcc ctggtgtgct
     1741 tgaccagttt gccactaaca ttcatcgcca tgggcctggt ggccgtggcc acgtcccgcc
     1801 tggcgaaaaa ggaggtgacc atgtatgctg gtgctgccgt ggtggctgaa ggcgctctct
     1861 ccggaattcg aacggtaaag gcttttgagg gcgaggcgaa ggaggtggct gcgtacaagg
     1921 agcgtgtggt tgctgccaag atcttgaata tcaagagaaa tatgttctcc ggaatcggat
     1981 tcggtctgct ttggttcttc ttcatctacg catcctatgc tctggccttc tggtatggag
     2041 tgggtctggt aatcgaatcc gcgtatgaga actacgatgc tggcaccatg atcaccgtat
     2101 tcttttccgt gatgatgggt tccatgaata ttggaatggc ggctccctat atcgaggcat
     2161 ttggcatcgc gcaagggcgc ctgccgaagg ttttccacat tatcgagcag attccggaaa
     2221 ttaatccatt gatggggagg gtaaaagtta atgagcccct taccaccatt gagtttaagg
     2281 aagtggagtt ccagtatccc acgcgaccgg aggtttctat tctgaacaaa ctgaacctga
     2341 aaatccatcg tggccagacg gtggctcttg ttggtccatc cggctgtggc aaatcaacct
     2401 gcatccaact ggtccagcga ttctatgacc cccaggctgg taatcttttg ttcaatggca
     2461 ccaatcttaa ggacctcgac atcaactggc tacgcttcct taggattgga gtggtgggtc
     2521 aggaaccaat cctcttcgcc acttccatat acgagaatat caggtacggt cgagaggacg
     2581 ccactcgcga ggagatcgag gcagcacgtg ccgcagccaa tgcagccatc tttattaaga
     2641 agctacccaa aggatatgat actttggtgg gagagcgagg agctcagttg tccggagggc
     2701 agaagcagag gatagccatt cgtcgcgcat tgattcgtga tcccgaaatc ttgctgttgg
     2761 atgaagccac atctgcattg gataccgcca gtgaggctaa agtacaagct gccctggaga
     2821 aagtcagcgc tggaaggacg accatcatcg tggcccaccg tttgtccacc gttcgtcggg
     2881 cagacaggat tgtggtgatc aacaagggtg aggtggtgga gagtggaacg catcaagagt
     2941 tgatggagtt gaaggatcat tactttaatc ttgtaaccac tcaactgggt gaggatgatg
     3001 gctcggtcct tagtcccact ggtgatatct acaagaactt tgacatcaag gatgaagatg
     3061 aggaagagat caaagtactt agcgaggacg aggacgaaga tgtgatggtc actgacgaga
     3121 aaaataagaa aaagaaaatg aagaaggtca aggaccccaa cgaggtgaaa cccatgttgg
     3181 aggtgatgaa aatgaacaaa ccggaatggc ttcagatcgc agtgggttgc atctcctccg
     3241 tgatcatggg ctgcgccatg cccatctttg ccgtgctctt tggtagcatc cttcagattc
     3301 tttcggttaa ggataatgat caatacgttc gcgaaaactc caaccagtac agcttgtact
     3361 tcttgattgc cggtatcgta gtgggcattg ccacgttcct acagatatac ttctttggta
     3421 tcgccggtga gagactgacg gaacgccttc gaggactcat gttcgaggcc atgctgcgcc
     3481 aggaggtggc ctggttcgat gacaaggcga acggtaccgg aagcctttgt gcccgactct
     3541 ccggagatgc tgctgcagtt cagggtgtaa gtgtaaattg gatttcacat ttgtagctta
     3601 cctaatgatt tcaatgttcc cccatgccac tggtcaacga attggaacga tcattcaatc
     3661 gatctccact ctggcattgg gcattgcctt gtccatgtat tacgagtgga gtctgggatt
     3721 ggtggccctt gcctttacgc ccttcatcct gatcgccttc tacatgcagc gtactctaat
     3781 ggcaaaagag aacatgcgtc cggccaagac catggagaac tgcaccaaac tggccgtcga
     3841 agtggtttcc aatattcgta ctgtagcttc tctgggtcgt gaggaaatgt tccaccagaa
     3901 ctacattggc atgctgattc cagctgttga ggtaggctct atatagtact ttttttttta
     3961 atttgttaat tcatttccaa tattatctct atagatttcc aagagaaata cccacttccg
     4021 aggattggtc tacggtctcg cccgttccct gatgttcttt gcctatgctg cttgcatgta
     4081 ttatggaaca tggtgtgtga ttcaccgcgg aattttgttt ggagatgtat tcaagtaagt
     4141 gacgaaatag cacacttaac ttgataaatt tcctaacacc tcatctctcc ccatttattt
     4201 tagggtatcc caagccgtga tcatgggtac ggcttccatt gccaatgcat tggcctttgc
     4261 tcccaatatg cagaagggag tttcggcagc caagaccatc tttacgttcc tccgccgcca
     4321 gccgtcgatt gtggaccgac cgggagtgtc tcgtgatcct tggcatagcg agggatatgt
     4381 tcgcttcgac aaggtgaagt tcagctatcc cacacgcagt gagattcagg tactcaaggg
     4441 tctggagctt gctgtgagca agggtcagaa gatcgcccta gtgggtccat cgggctgtgg
     4501 caagtccacg tgcatccagc tgatacagcg attctacgat gtagatgagg gagctaccct
     4561 tatagatgag tgtgatgtgc gcaatgtttc tatgaccaat ctgcgcaacc agttgggtat
     4621 cgtgtcgcag gagcccattc ttttcgatcg cacaatccga gagaatattt cctacggtga
     4681 caatgccagg aatgtgactg atcaggagat catctcggcg tgcaaaaagt ccaatatcca
     4741 cgaattcatc gctaacctgc ccttggtaag agcccctatc ttgacttttc tctcgaattt
     4801 ctagtaatat agtaccttat cccatagggt tatgatacaa gaatgggcga gaagggtgct
     4861 cagttgtccg gaggtcagaa gcagcgcata gctattgcac gagccctcat ccggaatccg
     4921 aaaatcatgc tgcttgacga ggccacttct gccttggacg ctgaaagtga aaaggttgga
     4981 gtacagagcc ctgttctata taggacttcc gtattcattt atctaattct tcttcaggtt
     5041 gtccaagatg cactcgatgc cgcctctgag ggacgtacca cgatcagtat agcccaccga
     5101 ctctccaccg ttgtccactc cgacgtaata ttcgtcttcg agaacggatt agtgtgtgaa
     5161 gctggagacc acaagcaact gcttgcgaat cggggtctct actacacgct ctacaaactt
     5221 cagagcggca gcatgtagaa gaaaaatctt aataggttta gccaatccaa ctagcttagt
     5281 gaattgcgaa caacatcatc aattatatgt acaaaaactc catagaagtt aacaacaaga
     5341 ttatttat
//