From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Escherichia coli smbA gene.
Date: 31 Dec 92 01:44:35 GMT
Distribution: bionet
Checksum: 20906 6

LOCUS       ECOSMBA      2277 bp ds-DNA             BCT       18-DEC-1992
DEFINITION  Escherichia coli smbA gene.
ACCESSION   D13334
KEYWORDS    EF-Ts; FRR; ribosome releasing factor;
            translation elongation factor.
SOURCE      Escherichia coli DNA.
  ORGANISM  Escherichia coli
            Prokaryotae; Gram-negative facultatively anaerobic rods;
            Enterobacteriaceae.
REFERENCE   1  (sites)
  AUTHORS   An,G., Bendiak,D.S., Mamelak,L.A. and Friesen,J.D.
  TITLE     Organization and nucleotide sequence of a new ribosomal operon in
            Escherichia coli containing the genes for ribosomal proteins S2 and
            elongation factors Ts
  JOURNAL   Nucleic Acids Res. 9, 4163-4172 (1981)
  STANDARD  full automatic
REFERENCE   2  (sites)
  AUTHORS   Ichikawa,S. and Kaji,A.
  TITLE     Molecular cloning and expression of ribosome releasing factor
  JOURNAL   J. Biol. Chem. 264, 20054-20059 (1989)
  STANDARD  full automatic
REFERENCE   3  (bases 1 to 2277)
  AUTHORS   Yamanaka,K., Ogura,T., Niki,H. and Hiraga,S.
  TITLE     Identification and characterization of the smbA gene, a suppressor
            of the mukB null mutant in Esherichia coli
  JOURNAL   J. Bacteriol. (1992) In press
  STANDARD  full automatic
COMMENT     Submitted (03-OCT-1992) to DDBJ by: Kunitoshi Yamanaka Dept. of
            Molecualr Cell Biology Inst. of Mol. Embryol. and Genet. Kumamoto
            University 4-24-1 Kuhonji Kumamoto 862 Japan Phone:  092-344-2111
            x6762 Fax:    096-371-2408

               [1]  sites. [2]  sites.
FEATURES             Location/Qualifiers
     CDS             <1..318
                     /gene="tsf"
                     /citation=[3]
                     /citation=[2]
                     /product="translation elongation factor EF-Ts"
                     /codon_start=1
                     /translation="EFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEGRMKK
                     FTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEVAAMS
                     KQS"
     CDS             465..1190
                     /gene="smbA"
                     /product="SmbA"
                     /codon_start=1
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     CDS             1482..2039
                     /gene="frr"
                     /product="ribosome releasing factor (FRR)"
                     /codon_start=1
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
BASE COUNT      606 a    517 c    634 g    520 t
ORIGIN      
        1 gaattcatca aaccggaaga cgtatccgct gaagtggtag aaaaagaata ccaggtacag
       61 ctggatatcg cgatgcagtc tggtaagccg aaagaaatcg cagagaaaat ggttgaaggc
      121 cgcatgaaga aattcaccgg cgaagtttct ctgaccggtc agccgttcgt tatggaacca
      181 agcaaaactg ttggtcagct gctgaaagag cataacgctg aagtgactgg cttcatccgc
      241 ttcgaagtgg gtgaaggcat cgagaaagtt gagactgact ttgcagcaga agttgctgcg
      301 atgtccaagc agtcttaatt atcaaaaagg agccgcctga gggcggcttc tttttgtgcc
      361 catcttgtaa attcagctaa cccttgtggg gctgcgctga aaagcgacgt acaatgtcgc
      421 tagtattaat tcatttcaat cgttgacagt ctcaggaaag aaacatggct accaatgcaa
      481 aacccgtcta taaacgcatt ctgcttaagt tgagtggcga agctctgcag ggcactgaag
      541 gcttcggtat tgatgcaagc atactggatc gtatggctca ggaaatcaaa gaactggttg
      601 aactgggtat tcaggttggt gtggtgattg gtgggggtaa cctgttccgt ggcgctggtc
      661 tggcgaaagc gggtatgaac cgcgttgtgg gcgaccacat ggggatgctg gcgaccgtaa
      721 tgaacggcct ggcaatgcgt gatgcactgc accgcgccta tgtgaacgct cgtctgatgt
      781 ccgctattcc attgaatggc gtgtgcgaca gctacagctg ggcagaagct atcagcctgt
      841 tgcgcaacaa ccgtgtggtg atcctctccg ccggtacagg taacccgttc tttaccaccg
      901 actcagcagc ttgcctgcgt ggtatcgaaa ttgaagccga tgtggtgctg aaagcaacca
      961 aagttgacgg cgtgtttacc gctgatccgg cgaaagatcc aaccgcaacc atgtacgagc
     1021 aactgactta cagcgaagtg ctggaaaaag agctgaaagt catggacctg gcggccttca
     1081 cgctggctcg tgaccataaa ttaccgattc gtgttttcaa tatgaacaaa ccgggtgcgc
     1141 tgcgccgtgt ggtaatgggt gaaaaagaag ggactttaat cacggaataa ttcccgtgat
     1201 ggataaataa gggtaagatt ccgcgtaagt atcgcggggg cgtaagtctg gttataaggc
     1261 gttattgttg caggcagttt ggtcacggcc agcgcgcagc aaccggagcg tacaaaagta
     1321 cgtgaggatg gcgagcactg cccggggcca aaatggcaaa taaaatagcc taataatcca
     1381 gacgattacc cgtaatatgt ttaatcaggg ctatacttag cacacttcca ctgtgtgtga
     1441 ctgtctggtc tgactgagac aagttttcaa ggattcgtaa cgtgattagc gatatcagaa
     1501 aagatgctga agtacgcatg gacaaatgcg tagaagcgtt caaaacccaa atcagcaaaa
     1561 tacgcacggg tcgtgcttct cccagcctgc tggatggcat tgtcgtggaa tattacggca
     1621 cgccgacgcc gctgcgtcag ctggcaagcg taacggtaga agattcccgt acactgaaaa
     1681 tcaacgtgtt tgatcgttca atgtctccgg ccgttgaaaa agcgattatg gcgtccgatc
     1741 ttggcctgaa cccgaactct gcgggtagcg acatccgtgt tccgctgccg ccgctgacgg
     1801 aagaacgtcg taaagatctg accaaaatcg ttcgtggtga agcagaacaa gcgcgtgttg
     1861 cagtacgtaa cgtgcgtcgt gacgcgaacg acaaagtgaa agcactgttg aaagataaag
     1921 agatcagcga agacgacgat cgccgttctc aggacgatgt acagaaactg actgatgctg
     1981 caatcaagaa aattgaagcg gcgctggcag acaaagaagc agaactgatg cagttctgat
     2041 ttcttgaacg acaaaaacgc cgctcagtag atccttgcgg atcggctggc ggcgttttgc
     2101 tttttattct gtctcaactc tggatgtttc atgaagcaac tcaccattct gggctcgacc
     2161 ggctcgattg gttgcagcac gctggacgtg gtgcgccata atcccgaaca cttccgcgta
     2221 gttgcgctgg tggcaggcaa aaatgtcact cgcatggtag aacagtgcct ggaattc
//