HDTenEightyP/NetNews huggingface dataset:
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Lentinus lepideus ribosomal RNA (17LR3) large subunit, partial.
Date: 19 Aug 92 10:02:04 GMT
Distribution: bionet
Checksum: 64091     2

LOCUS       LENRRLSF      218 bp ss-rRNA            RNA       19-AUG-1992
DEFINITION  Lentinus lepideus ribosomal RNA (17LR3) large subunit, partial.
ACCESSION   M98587
KEYWORDS    large subunit ribosomal RNA.
SOURCE      Lentinus lepideus (library: Duke culture collection 612) cDNA to
            rRNA.
  ORGANISM  Lentinus lepideus
            Eukaryota; Fungi; Eumycota; Basidiomycotina; Hymenomycetes;
            Agaricales; Tricholomataceae.
REFERENCE   1  (bases 1 to 218)
  AUTHORS   Hibbett,D.S. and Vilgalys,R.
  TITLE     Phylogenetic relationships of the basidiomycete genus Lentinus
            inferred from molecular and morphological characters
  JOURNAL   Unpublished (1992)
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     rRNA            <1..>218
                     /note="Aligns to region between positions 371 to 597
                     inSaccharomyces cerevisiae large subunit nuclear rRNA."
                     /product="large subunit ribosomal RNA"
                     /citation=[1]
BASE COUNT       52 a     41 c     68 g     57 t
ORIGIN
        1 aaagatgaaa agcactttgg aaagagagtt aaacatgacg tgaaattgtt gaaagggaaa
       61 tgcttgaagt cagtcgcgtt agccgggact cagccttgcc tttcgcttgg tgtattttcc
      121 ggttgacggg ccagcatcga ctttgaccgc tggataaggg tcaggggaat gtggcacctc
      181 cgggtgtgtt atagcctctg gtcgtataca gtggtcgg
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: H.sapiens dystrophin gene, exon 69.
Date: 20 Aug 92 10:03:06 GMT
Distribution: bionet
Checksum: 20954     2

LOCUS       HUMDYST10     247 bp ds-DNA             PRI       20-AUG-1992
DEFINITION  H.sapiens dystrophin gene, exon 69.
ACCESSION   M86893
KEYWORDS    Becker muscular dystrophy; Duchenne muscular dystrophy; dystrophin;
            integral membrane glycoprotein; membrane-associated protein.
SOURCE      Homo sapiens DNA.
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 247)
  AUTHORS   Roberts,R.G., Coffey,A.J., Bobrow,M. and Bentley,D.R.
  TITLE     Determination of the exon structure of the distal protion of the
            dystrophin gene by vectorette PCR
  JOURNAL   Genomics 13, 942-950 (1992)
  STANDARD  simple staff_entry
FEATURES             Location/Qualifiers
     exon            96..207
                     /number=69
                     /gene="dystrophin"
BASE COUNT       68 a     43 c     55 g     81 t
ORIGIN      chromosome X, map position Xp21
        1 ctcaaattag aacgtggtag aaggtttatt aaagagtgtt ctttgggaat ttgattcgaa
       61 gaaatacata cgtgtttgtt tttgctcttt atcaggtaca ggagtctaaa gcactttaat
      121 tatgacatct gccaaagctg ctttttttct ggtcgagttg caaaaggcca taaaatgcac
      181 tatcccatgg tggaatattg cactccggta agtttgacgc cagcctgacg tgagagttag
      241 ttcacct
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human (cdc25) mRNA.
Date: 22 Aug 92 10:00:51 GMT
Distribution: bionet
Checksum: 42977     2

LOCUS       HUMCDC        834 bp ss-mRNA            PRI       22-AUG-1992
DEFINITION  Human (cdc25) mRNA.
ACCESSION   M91815
KEYWORDS    .
SOURCE      Homo sapiens fetal cDNA to mRNA.
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 834)
  AUTHORS   Wei,W., Mosteller,R.D., Sanyal,P., Gonzales,E., McKinney,D.,
            Dasgupta,C., Li,P., Liu,B.-X. and Broek,D.
  TITLE     Identification of a mammalian gene structurally and functionally
            related to the CDC25 gene of Saccharomyces cerevisiae
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 89, 7100-7104 (1992)
  STANDARD  simple staff_entry
FEATURES             Location/Qualifiers
     polyA_site      834
                     /gene="cdc25"
BASE COUNT      241 a    212 c    181 g    200 t
ORIGIN
        1 cagctgaccc tgctggatca cctcgccttc aagaagattc cttatgagga gttcttcgga
       61 caaggatgga tgaaactgga aaagaatgaa aggacccctt atatcatgaa aaccactaag
      121 cacttcaatg acatcagtaa cttgattgct tcagaaatca tccgcaatga ggacatcaac
      181 gccagggtga gcgccatcgg gaagtgggtg gccgtagctg acatatgccg ctgcctccac
      241 aactacaatg ccgtactgga gatcacctgc tccatgaacc gcagtgcaat cttccggctc
      301 aaaaagacgt ggctcaaagt ctctaagcag actaaagctt tgattgataa gctccaaaag
      361 cttgtgtcat cagagggcag atttaagaat ctcagagaag ctctgaaaaa ttgtgaccca
      421 ccctgtgtcc cttacctggg gatgtacctc accgacctgg ccttcatcga ggaggggacg
      481 cccaattaca cggaagacgg cctggtcaac ttctccaaga tgaggatgat atcccatatt
      541 atccgagaga ttcgccagtt tcaacaaact gcctacaaaa tagagcacca agcaaaggta
      601 acgcaatatt tactggacca atcttttgta atggatgaag aaagcctcta cgagtcttct
      661 ctccgaatag aaccaaaact ccccacctga agctgtgccc agacccagac cagctgctcc
      721 cggggacatg tgctagatga tactgtacat attcgtttgg tttcactgga ttttcttctt
      781 cagtatgtgc ttctccaaga aatacaaatc gtccttgttc ttagattcct gtag
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human chromosome 4 sequence-tagged site STS4-428
Date: 21 Aug 92 10:10:14 GMT
Distribution: bionet
Checksum: 34473     3

LOCUS       HUM4STS428    430 bp ds-DNA             PRI       21-AUG-1992
DEFINITION  Human chromosome 4 sequence-tagged site STS4-428
ACCESSION   L00854
KEYWORDS    STS; human chromosome 4; sequence tagged site.
SOURCE      Homo sapiens, clone p4-452 from a chromosome 4 plasmid library;
            vector pBluescript II KS + (Stratagene)
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 430)
  AUTHORS   Goold,R.D., diSibio,G., Dugaiczyk,A., Smith,K.A., Xu,H.,
            Magrane,G., Lang,D.B., Cox,D.R., Masters,S.B. and Myers,R.M.
  TITLE     Sequence-Tagged Sites for Human Chromosome 4
  JOURNAL   Unpublished (1992) see COMMENT for author address
  STANDARD  full automatic
COMMENT     PCR components: 25 ng of human genomic DNA, 10 pmol of each
            oligonucleotide, 200 micro-M dNTPs, 0.25 U Taq  polymerase (Cetus)
            in 10 micro-l of 50 mM KCl-20 mM  Tris-HCl, pH 8.3 (at room temp),
            2.5 mM MgCl-2.  Initial denaturation at 94degC for 1.5 min, then 30
            cycles of  94degC for 15 sec, 62degC for 23 sec, and 72degC for 30
            sec, followed by a final extension at 72degC for 3.5 min, using a
            Perkin-Elmer 9600 thermocycler. PCR-amplified product size 292 bp.
            STS sequence contains a simple sequence tandem repeat (CA).
            Sequence submitted by:
            Human Genome Mapping Center
            Box 0925
            University of California San Francisco
            San Francisco, CA 94143-0925  USA
            Phone: (415) 502-1612   Fax: (415) 476-8391
            e-mail: hgmcprobes@cgl.ucsf.edu
FEATURES             Location/Qualifiers
     misc_feature    25..49
                     /label=Primer1
     misc_feature    complement(290..316)
                     /label=Primer2
BASE COUNT      140 a     79 c     56 g    131 t     24 others
ORIGIN
        1 gatcagaacc tgaagacccc agtgcaccac attagacaac tgtaaatagt acattaacat
       61 gtgatgcaca cacacacaca cacacacaca cacacacaag cgcatcttag atttctgttt
      121 ctatattgat ataaaaataa tttancatca ntgaaggaca antccanttt tttaatttat
      181 tttattacct aaatagtgtc ctatttagga tattaanngg gatatntttn accaaattat
      241 gatatataaa gattaattag aaaaacactt atgtattttc tcagtcagnt gaactacatt
      301 ttagtggagt atatttctnn ttactaatgc aatnnggaca tggttattct caccnaccnc
      361 aanncncctc caccttaata gnnggtgnnt tantgcaatt tatgtgacag gtntgggttc
      421 tcagcatcca
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human immunodeficiency virus type 2 (73POLDSU) polymerase fragment.
Date: 18 Aug 92 10:01:25 GMT
Distribution: bionet
Checksum: 44919     2

LOCUS       HIV7POLDSU    708 bp ss-RNA             VRL       18-AUG-1992
DEFINITION  Human immunodeficiency virus type 2 (73POLDSU) polymerase fragment.
ACCESSION   M87145
KEYWORDS    polymerase.
SOURCE      Human immunodeficiency virus type 2 uncultured blood mononuclear
            cells (9/90 sample) RNA.
  ORGANISM  Human immunodeficiency virus type 2
            Viridae; ss-RNA enveloped viruses; Positive strand RNA virus;
            Retroviridae; Lentivirinae.
REFERENCE   1  (bases 1 to 708)
  AUTHORS   Gao,F., Yue,L., White,A.T., Pappas,P.G., Barchue,J., Hanson,A.P.,
            Greene,B.M., Sharp,P.M., Shaw,G.M. and Hahn,B.H.
  TITLE     Human infection by genetically-diverse SIVsm-related HIV-2 in West
            Africa
  JOURNAL   Nature (1992) In press
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     misc_feature    1..708
                     /note="PCR derived HIV-2 polymerase fragment"
                     /note="putative"
                     /citation=[1]
BASE COUNT      279 a    122 c    164 g    143 t
ORIGIN
        1 catggggata gtagctggac agccaacaga aacagagtca ccaatagtaa ataagataat
       61 tgaagaaatg atcaaaaagg aagcaatata tgtaggatgg gtgccagctc acaaaggact
      121 aggtggtaat caggaagtag accacctagt aagtcaggga attaggcagg ttctattcct
      181 agaaaagata gaaccagcac aagaagaaca tgaaaaatat catggcaatg taaaagagct
      241 ggttcataaa tttggaattc cacaactagt ggcaaaacaa atagtaaact cctgtgataa
      301 atgtcaacaa aaaggagaag ctgttcatgg acaggtaaat gcagaactag ggacatggca
      361 aatggactgt acacatttag aagggaaagt cataatagta gcagtccatg tagccagtgg
      421 gtttatagaa gcagaggtaa taccccaaga aacaggaaga caaacagccc tcttcctgtt
      481 aaagctggca agcagatggc ccattacaca cttgcacaca gacaatggta ccaactttac
      541 ttcacaagat gtaaagatgg tggcttggtg ggtaggaata gaacaaacct ttggagtacc
      601 ctacaaccca caaagtcagg gagtagtaga agcaatgaac catcatctaa aaaatcaaat
      661 agataggatc agagatcagg cagtatcaat agaaacagtt gtgctaat
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Gallus gallus Ig rearranged L-chain gene V-J-region, partial cds.
Date: 21 Aug 92 10:05:59 GMT
Distribution: bionet
Checksum: 25894     3

LOCUS       CHKIGLAZI     431 bp ds-DNA             VRT       21-AUG-1992
DEFINITION  Gallus gallus Ig rearranged L-chain gene V-J-region, partial cds.
ACCESSION   M96973
KEYWORDS    immunoglobulin light chain; joining region; processed gene;
            variable region.
SOURCE      Gallus gallus (individual_isolate REV-T (CSV), strain SC line
            (Hyline International, Dallas Center, Iowa), sub_species
            domesticus) (library: TB2(TE8)) day 12 embryo bursa DNA.
  ORGANISM  Gallus gallus
            Eukaryota; Animalia; Chordata; Vertebrata; Aves; Neornithes;
            Neognathae; Galliformes; Phasianidae.
REFERENCE   1  (bases 1 to 431)
  AUTHORS   Benatar,T., Tkalee,L. and Ratcliffe,M.J.
  TITLE     Stochastic rearrangement of immunoglobulin variable-region genes in
            chicken B-cell development
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 89, 7615-7619 (1992)
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     intron          <1..105
                     /gene="IgL"
     CDS             106..>431
                     /note="V-J-region"
                     /product="immunoglobulin light chain"
                     /gene="IgL"
                     /codon_start=3
                     /translation="SLVQAALTQPSSVSANPGETVKITCSGDRSYYGWYQQKAPGSAP
                     VTVIYANTNRPSDIPSRFSGSKSGSTATLTITGVQADDEAVYYCGSADSSSTDGIFGA
                     GTTLTV"
     mat_peptide     123..>431
                     /note="V-J-region"
                     /product="immunoglobulin light chain"
                     /gene="IgL"
                     /codon_start=1
     misc_recomb     396..398
                     /note="VJ junction"
BASE COUNT       81 a    138 c    121 g     91 t
ORIGIN
        1 gggactgtgg gcacggggct ctgtcccatt gctgcgcggg cagggctgtg cgtgcggggc
       61 cgtcactgat tgccgttttc tcccctctct cctctccctc tccaggttcc ctggtgcagg
      121 cagcgctgac tcagccgtcc tcggtgtcag caaacccggg agaaaccgtc aagatcacct
      181 gctccgggga taggagctac tatggctggt accagcagaa ggcacctggc agtgcccctg
      241 tcactgtgat ctatgctaac accaacagac cctcggacat cccttcacga ttctccggtt
      301 ccaaatccgg ctccacagcc acattaacca tcactggggt ccaagccgac gacgaggctg
      361 tctattactg tgggagtgca gacagcagca gtactgatgg tatatttggg gccgggacaa
      421 ccctgaccgt c
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Rattus norvegicus phosphorylase kinase gene, exon 1.
Date: 15 Aug 92 10:03:06 GMT
Distribution: bionet
Checksum: 47721     3

LOCUS       RATPHOSPRY    850 bp ds-DNA             ROD       15-AUG-1992
DEFINITION  Rattus norvegicus phosphorylase kinase gene, exon 1.
ACCESSION   M98827
KEYWORDS    catalytic subunit; phosphorylase kinase.
SOURCE      Rattus norvegicus (strain Wistar) DNA.
  ORGANISM  Rattus norvegicus
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Rodentia; Myomorpha; Muridae; Murinae.
REFERENCE   1  (sites)
  AUTHORS   Cawley,K.C., Ramachandran,C., Gorin,F.A. and Walsh,D.A.
  TITLE     Nucleotide sequence of cDNA encoding the catalytic subunit of
            phosphorylase kinase from rat soleus muscle
  JOURNAL   Nucleic Acids Res. 16, 2355-2356 (1988)
  STANDARD  full staff_review
REFERENCE   2  (bases 1 to 850)
  AUTHORS   Cawley,K.C., Akita,C.G., Angelos,K.L. and Walsh,D.A.
  TITLE     Characterization of the gene for rat phosphorylase kinase catalytic
            subunit
  JOURNAL   Unpublished (1992)
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     5'UTR           join(402..556,M98826:731..764)
                     /evidence=EXPERIMENTAL
                     /citation=[2]
                     /citation=[1]
                     /partial
     exon            402..556
                     /number=1
     intron          557..850
                     /number=1
                     /cons_splice=(5'site:yes,3'site:no)
                     /note="entire intron 1 is ~3.3kb"
                     /evidence=EXPERIMENTAL
                     /citation=[2]
                     /citation=[1]
                     /partial
BASE COUNT      192 a    247 c    178 g    233 t
ORIGIN
        1 ctttgtttgt tttgtttttc caggccaggt ttcttttctt ttcttttttt tttttttttt
       61 ggagctgggg accgaaccca gggcctccgt gtgctaggca agcgctctac cactgagcca
      121 aatccccaac cccccaggcc aggtttctat gtgtaacttt ctgcctgtca tggaaatcac
      181 tcggaactct aaaaatctac ctgcctctgc ctccacactg gaggcgcttt cctcagcaaa
      241 agccatttgc tttgtcccaa ttcacgtgtg tttccctcag cctccgccag gtgtcacccc
      301 tgatgtagtt ttgccccagg cctatccctc ctggaccttg gcctgcatcc caggtttgca
      361 gttgtgattt atttttggca gaccggcctt ccatcaccaa gtaccaaagt cccagaagag
      421 gagcaagata gcaactctaa caagtgtctg ctcaggacac agcttcaatc ctctgtgaca
      481 gtccgctgtc tcccaagggc tccaggagct ttggaattcc tgtcacttcc attcaagagc
      541 ctacaagaac ctaactgtaa gtagccaggc agcagtcggg gagcaatagg gttatgaggc
      601 aaaaacaaga ccccttcccc aggtccgttt gacccatttc tcctcagacc tggagagcac
      661 agattctcag aacacctgtt gggcagttat ctacagatca tgcatgtggc tgtgtcctct
      721 gactagcttt ctgggatatc gaattccaaa acgttggacc atctactcat atttagaact
      781 ccctcacacc tccagggagt cttggaacaa gcgtaacccg ctaatccacg cccagaataa
      841 agatccatgg
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human immunodeficiency virus type 2 (FOENVA1) envelope protein
Date: 18 Aug 92 10:01:26 GMT
Distribution: bionet
Checksum: 46121     2

LOCUS       HIVENVA1      453 bp ss-RNA             VRL       18-AUG-1992
DEFINITION  Human immunodeficiency virus type 2 (FOENVA1) envelope protein
            fragment.
ACCESSION   M87070
KEYWORDS    envelope protein.
SOURCE      Human immunodeficiency virus type 2 uncultured blood mononuclear
            cells (11/89 sample) RNA.
  ORGANISM  Human immunodeficiency virus type 2
            Viridae; ss-RNA enveloped viruses; Positive strand RNA virus;
            Retroviridae; Lentivirinae.
REFERENCE   1  (bases 1 to 453)
  AUTHORS   Gao,F., Yue,L., White,A.T., Pappas,P.G., Barchue,J., Hanson,A.P.,
            Greene,B.M., Sharp,P.M., Shaw,G.M. and Hahn,B.H.
  TITLE     Human infection by genetically-diverse SIVsm-related HIV-2 in West
            Africa
  JOURNAL   Nature (1992) In press
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     misc_feature    1..453
                     /note="PCR derived HIV-2 envelope fragment"
                     /gene="env"
                     /note="putative"
                     /citation=[1]
BASE COUNT      160 a     77 c    105 g    111 t
ORIGIN
        1 gacgtggtca agagacaaca agaattgttg cgactgaccg tctgggggac taaaaacctc
       61 cagactagag tcactgctat cgagaagtac ctaaaggacc aggcgcagct aaattcatgg
      121 ggatgcgcgt tcaggcaggt ctgccacacc actgtagaat ggccaaatag tactctcaca
      181 cctgactaga acaatatgac ttggcaggag tgggagagac aggttgattt cctagaggca
      241 aatataacac aattattaga ggaagcacaa attcaacaag aaaagaacat gtatgagtta
      301 caaaaactaa ataactggga tatatttggc aactggtttg accttacttc ttggattaag
      361 tatatacagt atggagtctt catagtagta ggtataatac tgttaagaat aataatctat
      421 gtagtacaaa tgttagctaa gttaagtaag gga
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Mouse T cell receptor rearranged alpha-chain variable region,
Date: 15 Aug 92 10:02:39 GMT
Distribution: bionet
Checksum: 35465     2

LOCUS       MUSTCRA6C     345 bp ss-mRNA            ROD       15-AUG-1992
DEFINITION  Mouse T cell receptor rearranged alpha-chain variable region,
            N_region, joining region, and constant region mRNA.
ACCESSION   M98570
KEYWORDS    T-cell receptor; alpha-subunit receptor; constant region;
            joining region; variable region.
SOURCE      Mus musculus (strain BALB/cKh) adult spleen cDNA to mRNA.
  ORGANISM  Mus musculus
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Rodentia; Myomorpha; Muridae; Murinae.
REFERENCE   1  (bases 1 to 345)
  AUTHORS   Solheim,J.C., Martinko,J.M., Alexander-Miller,M. and Connolly,J.M.
  TITLE     Biased T Cell Receptor Usage by Ld-restricted Tum-Peptide-specific
            Cytotoxic T Lymphocyte Clones
  JOURNAL   Unpublished (1992)
  STANDARD  full staff_entry
BASE COUNT      111 a     86 c     68 g     80 t
ORIGIN      Chromosome 14
        1 acagcaagtg gggaaatagt tttccttatt cgtcaggact cttacaaaaa ggaaaatgca
       61 acagtgggtc attattctct gaactttcag aagccaaaaa gttccatcgg actcatcatc
      121 accgccacac agattgagga ctcagcagta tatttctgtg ctatgagaga gggcaactat
      181 ggaaatgaga aaataacttt tggggctgga accaaactca ccattaaacc caacatccag
      241 aacccagaac ctgctgtgta ccagttaaaa gatcctcggt ctcaggacag caccctctgc
      301 ctgttcaccg actttgactc ccaaatcaat gtgccgaaaa ccatg
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human chromosome 4 sequence-tagged site STS4-135
Date: 21 Aug 92 10:06:52 GMT
Distribution: bionet
Checksum: 46361     3

LOCUS       HUM4STS135    396 bp ds-DNA             PRI       21-AUG-1992
DEFINITION  Human chromosome 4 sequence-tagged site STS4-135
ACCESSION   M95259
KEYWORDS    STS; human chromosome 4; sequence tagged site.
SOURCE      Homo sapiens, clone C4-167 from Los Alamos National Laboratory
            chromosome 4 cosmid library, plate 4-1-2R, pos5B; vector SuperCos 1
            (Stratagene)
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 396)
  AUTHORS   Goold,R.D., diSibio,G., Dugaiczyk,A., Smith,K.A., Xu,H.,
            Magrane,G., Lang,D.B., Cox,D.R., Masters,S.B. and Myers,R.M.
  TITLE     Sequence-Tagged Sites for Human Chromosome 4
  JOURNAL   Unpublished (1992) see COMMENT for author address
  STANDARD  full automatic
COMMENT     PCR components: 25 ng of human genomic DNA, 10 pmol of each
            oligonucleotide, 200 micro-M dNTPs, 0.25 U Taq  polymerase (Cetus)
            in 10 micro-l of 50 mM KCl-20 mM  Tris-HCl, pH 8.3 (at room temp),
            2.5 mM MgCl-2.  Initial denaturation at 94degC for 1.5 min, then 30
            cycles of  94degC for 15 sec, 62degC for 23 sec, and 72degC for 30
            sec, followed by a final extension at 72degC for 3.5 min, using a
            Perkin-Elmer 9600 thermocycler. PCR-amplified product size 113 bp.
            Sequence submitted by:
            Human Genome Mapping Center
            Box 0925
            University of California San Francisco
            San Francisco, CA 94143-0925  USA
            Phone: (415) 502-1612   Fax: (415) 476-8391
            e-mail: hgmcprobes@cgl.ucsf.edu
FEATURES             Location/Qualifiers
     misc_feature    13..35
                     /label=Primer1
     misc_feature    complement(104..125)
                     /label=Primer2
BASE COUNT      101 a     84 c     90 g    102 t     19 others
ORIGIN
        1 agagtgaaaa aagatctcgt ttctgtacgc tcatgtttat tgtaacacta ttcacaatag
       61 tcaagtatag tttngngata ctattagaat caacctaagt gtccatcaag agacaaatgg
      121 gctgggtgca gtggctcata cctgttctcc caggactttg gggggncaat acaggtggat
      181 tgcttgagcc caggggtttg ngaccagctt ggacatgatg aaactccatc tctacaaaaa
      241 atncaaaatt tagctaggct gggtagccca tcactgtggt ccccagctac tcaggagact
      301 gaggcaggag gattgcttga ncctnanagg ccgncgctnt agtnanctna ctttgngccn
      361 ntacactttc agcctcggta tcacagtnna ctcttn
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Zea mays copia-like retrotransposon reverse transcriptase gene,
Date: 14 Aug 92 10:01:04 GMT
Distribution: bionet
Checksum: 05560     2

LOCUS       MZECOPIAB     274 bp ds-DNA             PLN       14-AUG-1992
DEFINITION  Zea mays copia-like retrotransposon reverse transcriptase gene,
            partial cds.
ACCESSION   M94482
KEYWORDS    copia-like element; retrotransposon; reverse transcriptase.
SOURCE      Zea mays DNA.
  ORGANISM  Zea mays
            Eukaryota; Plantae; Embryobionta; Magnoliophyta; Liliopsida;
            Commelinidae; Cyperales; Poaceae.
REFERENCE   1  (bases 1 to 274)
  AUTHORS   Voytas,D.F., Cummings,M.P., Konieczny,A., Ausubel,F.M. and
            Rodermel,S.R.
  TITLE     Copia-like retrotransposons are ubiquitous among plants
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 89, 7124-7128 (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     CDS             6..269
                     /note="PCR product"
                     /product="reverse transcriptase"
                     /codon_start=1
                     /translation="KTAFLHGDLEEDIYMDQPEGFIVPGKEDYVCRLKKSLYGLKQSP
                     RQWYKRFDSFMLSKGFQRSQYDSCVYLKFVNGSPTYLLLYVDDM"
                     /partial
BASE COUNT       77 a     40 c     65 g     92 t
ORIGIN
        1 gtgacaagac tgctttttta catggtgatt tggaggagga catttatatg gaccagccag
       61 aggggttcat tgttcctgga aaagaagact atgtgtgcag gttgaagaag tctttatatg
      121 gtctgaaaca atctcccaga caatggtata agaggtttga ttcatttatg ctttctaagg
      181 gctttcagag gtctcaatat gatagttgtg tttatctaaa atttgttaat ggatcaccta
      241 catatttgct actatacgtg gacgatatgg atcc
//