From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Bacillus stearothermophilus proton glutamate symport protein (gltP)
Date: 6 Nov 92 18:05:24 GMT
Distribution: bionet
Checksum: 00266 4

LOCUS       BACGLTPA     1537 bp ds-DNA             BCT       28-OCT-1992
DEFINITION  Bacillus stearothermophilus proton glutamate symport protein (gltP)
            gene, complete cds.
ACCESSION   M86508
KEYWORDS    gltP gene; glutamate/aspartate transport protein;
            proton glutamate symport protein.
SOURCE      Bacillus stearothermophilus (library: ATCC 7954) DNA.
  ORGANISM  Bacillus stearothermophilus
            Prokaryotae; Firmicutes; Endospore-forming rods and cocci;
            Bacillaceae.
REFERENCE   1  (bases 1 to 1537)
  AUTHORS   Tolner,B., Poolman,B. and Konings,W.N.
  TITLE     Characterization and functional expression in Eschicheria coli of
            the sodium/proton/glutamate syport proteins of bacillus
            stearothermophilus and bacillus caldotenax get
  JOURNAL   Mol. Microbiol. 6, 2845-2856 (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     -35_signal      31..36
                     /gene="gltP"
     -10_signal      54..59
                     /gene="gltP"
     RBS             94..105
                     /gene="gltP"
     terminator      1379..1418
                     /note="rho-independent"
                     /gene="gltP"
     CDS             110..1375
                     /gene="gltP"
                     /product="proton glutamate symport protein"
                     /codon_start=1
                     /translation="MRKIGLAWQIFIGLILGIIVGAIFYGNPKVATYLQPIGDIFLRL
                     IKMIVIPIVISSLVVGVASVGDLKKLGKLGGKTIIYFEIITTIAIVVGLLAANIFQPG
                     TGVNMKSLEKTDIQSYVDTTNEVQHHSMVETFVNIVPKNIFESLTKGDMLPIIFFSVM
                     FGLGVAAIGEKGKPVLQFFQGTAEAMFYVTNQIMKFAPFGVFALIGVTVSKFGVESLI
                     PLSKLVIVVYATMVFFIFVVLGGVAKLFGINIFHIIKILKDELILAYSTASSETVLPK
                     IMEKMENFGCPKAITSFVIPTGYSFNLDGSTLYQALAAIFIAQLYGIDMPISQQISLL
                     LVLMVTSKGIAGVPGVSFVVLLATLGTVGIPIEGLAFIAGIDRILDMARTAVNVIGNS
                     LAAIIMSKWEGQYNEEKGKQYIAQLQQSA"
BASE COUNT      440 a    247 c    351 g    499 t
ORIGIN      
        1 gaattcaatg acaatattat atgaatattc ttgacataac gaaaatatga tagtagaata
       61 gttttgtctc tttttgtcta aaaatgtcat aaaagaaagg ggcgaatgaa tgagaaaaat
      121 tggattagct tggcaaattt ttattggtct cattctaggg attatcgtgg gagccatttt
      181 ttacggaaat ccgaaggttg ccacttattt acagcctatt ggagatattt tccttcgttt
      241 aatcaaaatg attgtcattc cgattgttat ttctagcctt gtagttggag tcgccagcgt
      301 tggggatttg aagaagcttg gaaaattagg cggcaaaacg attatttatt tcgagattat
      361 cacaacgatc gcgattgtcg tcggtttatt ggcagcgaat atttttcagc cagggaccgg
      421 cgttaatatg aaatcattag aaaaaaccga tattcaaagc tatgttgata caacaaacga
      481 agtgcagcat cattcgatgg tggaaacttt tgttaatatt gttccaaaaa atatttttga
      541 atcgttaacc aaaggggata tgctgccgat cattttcttc tctgttatgt tcggtttagg
      601 agtagcggcg attggcgaaa aaggaaagcc agttcttcaa tttttccaag gtacagcaga
      661 agcgatgttt tatgtaacaa accaaattat gaagtttgcg ccgttcggcg tgtttgcgct
      721 gattggtgta accgtttcta agtttggggt agagtcgctt attccgctca gcaagctcgt
      781 cattgttgtt tacgcaacga tggtgttctt tatctttgtc gtgcttggcg gtgttgctaa
      841 gttatttggc ataaatattt ttcatattat aaaaattttg aaagatgagt taattcttgc
      901 ttatagtaca gcaagttcgg aaaccgttct tccgaaaatt atggagaaaa tggagaattt
      961 cggttgtcca aaagcgatta catcctttgt cattccgaca gggtattctt ttaacttaga
     1021 cggttctacg ttatatcagg cgttggcggc catttttatc gcgcagttgt acggtattga
     1081 catgccgatt tctcaacaaa tctcgctttt gcttgtgtta atggtgactt cgaaaggaat
     1141 cgctggggtg ccgggtgtat cctttgtcgt gctgcttgct acgttaggca cggttgggat
     1201 tccgatagaa ggattagcat ttatcgctgg aatcgaccgt attttagata tggcgcgcac
     1261 agcagtgaat gttattggca actcgttagc agcgatcatt atgtcaaaat gggaaggcca
     1321 atataacgaa gaaaaaggaa aacaatacat cgcgcaattg cagcaaagtg cataaatgct
     1381 gaaaaagctg tcctgctaga aaaggacagc tttttcagta taaggactta tttttttctt
     1441 ccacgtagta tagtataatt tggtgctgaa taaggttgta atgagatgct tggctaagag
     1501 tacataatac ataggaggaa gaaccatgca cgaattc
//