From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Erwinia carotovora polygalacturonase (peh1) gene, complete cds.
Date: 26 Aug 92 10:00:31 GMT
Distribution: bionet
Checksum: 63936     4

LOCUS       ERWPLYGLNS   1209 bp ds-DNA             BCT       26-AUG-1992
DEFINITION  Erwinia carotovora polygalacturonase (peh1) gene, complete cds.
ACCESSION   M83222
KEYWORDS    polygalacturonase.
SOURCE      Erwinia carotovora (sub_species carotovora) DNA.
  ORGANISM  Erwinia carotovora
            Prokaryotae; Gram-negative facultatively anaerobic rods;
            Enterobacteriaceae.
REFERENCE   1  (bases 1 to 1209)
  AUTHORS   Laing,E. and Pretorius,I.S.
  TITLE     Primary structure and expression of the Erwinia carotovora subsp.
            carotovora polygalacturonase-encoding gene in Saccharomyces
            cerevisiae
  JOURNAL   Unpublished (1992)
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     CDS             1..1209
                     /function="enzyme that hydrolyzes pectate"
                     /EC_number="3.2.1.15"
                     /product="polygalacturonase"
                     /gene="peh1"
                     /evidence=EXPERIMENTAL
                     /codon_start=1
                     /translation="MEYQSGKRVLSLSLGLIGLFSASAFASDSRTVSEPKTPSSCTVL
                     KADSSTATSTIQKALNNCGQGKAVKLSAGSSSVFLSGPLSLPSGVSLLIDKGVTLRAV
                     NNAKSFENAPSSCGVVDTNGKGCDAFITATSTTNSGIYGPGTIDGQGGVKLQDKKVSW
                     WDLAADAKVKKLKQNTPRLIQINKSKNFTLYNVSLINSPNFHVVFSDGDGFTAWKTTI
                     KTPSTARNTDGIDPMSSKNITIAHSNISTGDDNVAIKAYKGRSETRNISILHNEFGTG
                     HGMSIGSETMGVYNVTVDDLVMTGTTNGLRIKSDKSAAGVVNGVRYSNVVMKNVAKPI
                     VIDTVYEKKEGSNVPDWSDITFKDITSQTKGVVVLNGENAKKPIEVTMKNVKLTSDST
                     WQIKNVNVKK"
                     /citation=[1]
BASE COUNT      358 a    254 c    319 g    278 t
ORIGIN
        1 atggaatatc aatcaggcaa gcgcgtttta tcattatcac tggggcttat cggtttgttt
       61 agcgcatcgg catttgcatc tgattcccga acggtgagtg aaccgaaaac accgtcttcc
      121 tgtacggtgc ttaaagcgga cagtagtact gccacgagta caattcaaaa agcgctgaat
      181 aattgcgggc aaggaaaagc ggtaaagctg agcgcaggga gttcatccgt ttttctgagt
      241 ggcccgcttt ctctaccttc tggcgtgagc ttattaatcg acaaaggagt aaccctacgt
      301 gctgtgaata atgccaagtc ttttgagaat gcgccgtcat cctgtggcgt ggtggataca
      361 aacggtaaag gctgcgatgc atttattacc gctacaagca cgacgaatag cggaatctat
      421 gggccgggca ccattgatgg acaagggggc gtgaagcttc aggataaaaa ggtgagctgg
      481 tgggatctgg ctgccgatgc caaagtgaaa aagttaaaac agaatacccc ccggctgatt
      541 cagattaata agagcaagaa tttcacgctg tataacgtct ctctcattaa ttctccgaac
      601 ttccacgtcg tgttcagcga tggtgacggc ttcaccgcgt ggaaaaccac gatcaaaacg
      661 ccgtccaccg ccagaaacac tgacggtatc gatccgatgt cgtcaaagaa catcaccatt
      721 gcccacagta atatttcgac gggcgacgat aatgtggcga tcaaagccta taaaggtcgt
      781 tctgagacac gcaatatttc cattttgcat aatgaatttg gaacgggaca tggcatgtcg
      841 atcggtagcg aaacgatggg agtttataac gtgacggtcg atgatctggt tatgactggc
      901 accacgaatg gattacgtat taaaagcgac aaatcagcag ccggcgttgt caatggtgtt
      961 cggtatagca acgtagtcat gaagaacgtg gcgaaaccga tcgtgattga cacggtatat
     1021 gagaaaaaag agggaagtaa tgttcctgac tggagcgaca ttacgtttaa ggatattacg
     1081 tctcaaacca aaggtgtggt ggtgctgaac ggcgagaatg cgaaaaagcc gatagaagtg
     1141 acgatgaaga acgtcaaact gacgagtgac agcacatggc aaatcaagaa cgtcaacgtc
     1201 aagaagtag
//