From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human alpha-L-iduronidase gene, exons 3 through 14.
Date: 29 Dec 92 20:55:32 GMT
Distribution: bionet
Checksum: 44704 13

LOCUS       HUMALIDN02   4480 bp ds-DNA             PRI       11-DEC-1992
DEFINITION  Human alpha-L-iduronidase gene, exons 3 through 14.
ACCESSION   M95740
KEYWORDS    alpha-L-iduronidase.
SEGMENT     2 of 2
SOURCE      Homo sapiens DNA.
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chorqata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 4480)
  AUTHORS   Scott,H.S., Guo,X.-H., Hopwood,J.J. and Morris,C.P.
  TITLE     Structure and sequence of the human alpha-L-iduronidase gene
  JOURNAL   Genomics 13, 1311-1313 (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     exon            427..512
                     /number=3
                     /function="cleavage of heparan and dermatan sulphate"
                     /EC_number="3.2.1.76"
                     /product="alpha-L-iduronidase"
                     /gene="IDUA"
                     /evidence="EXPERIMENTAL"
                     /citation=[1]
     intron          513..696
                     /number=3
                     /standard_name="intron 3"
                     /gene="IDUA"
                     /evidence="EXPERIMENTAL"
                     /citation=[1]
     exon            697..804
                     /number=4
                     /gene="IDUA"
                     /evidence="EXPERIMENTAL"
                     /citation=[1]
     intron          805..1282
                     /number=4
                     /gene="IDUA"
                     /evidence="EXPERIMENTAL"
                     /citation=[1]
     exon            1283..1378
                     /number=5
                     /gene="IDUA"
                     /evidence="EXPERIMENTAL"
                     /citation=[1]
     intron          1379..1493
                     /number=5
                     /gene="IDUA"
                     /evidence="EXPERIMENTAL"
                     /citation=[1]
     exon            1494..1696
                     /number=6
                     /gene="IDUA"
                     /evidence="EXPERIMENTAL"
                     /citation=[1]
     intron          1687..1796
                     /number=6
                     /gene="IDUA"
                     /evidence="EXPERIMENTAL"
                     /citation=[1]
     exon            1797..1976
                     /number=7
                     /gene="IDUA"
                     /evidence="EXPERIMENTAL"
                     /citation=[1]
     intron          1977..2083
                     /number=7
                     /gene="IDUA"
                     /evidence="EXPERIMENTAL"
                     /citation=[1]
     exon            2084..2300
                     /number=8
                     /gene="IDUA"
                     /evidence="EXPERIMENTAL"
                     /citation=[1]
     intron          2301..2546
                     /number=8
                     /gene="IDUA"
                     /evidence="EXPERIMENTAL"
                     /citation=[1]
     exon            2547..2759
                     /number=9
                     /gene="IDUA"
                     /evidence="EXPERIMENTAL"
                     /citation=[1]
     intron          2760..2850
                     /number=9
                     /gene="IDUA"
                     /evidence="EXPERIMENTAL"
                     /citation=[1]
     exon            2851..2972
                     /number=10
                     /gene="IDUA"
                     /evidence="EXPERIMENTAL"
                     /citation=[1]
     intron          2973..3159
                     /number=10
                     /gene="IDUA"
                     /evidence="EXPERIMENTAL"
                     /citation=[1]
     exon            3160..3285
                     /number=11
                     /gene="IDUA"
                     /evidence="EXPERIMENTAL"
                     /citation=[1]
     intron          3286..3363
                     /number=11
                     /gene="IDUA"
                     /evidence="EXPERIMENTAL"
                     /citation=[1]
     exon            3364..3440
                     /number=12
                     /gene="IDUA"
                     /evidence="EXPERIMENTAL"
                     /citation=[1]
     intron          3441..3826
                     /number=12
                     /gene="IDUA"
                     /evidence="EXPERIMENTAL"
                     /citation=[1]
     exon            3827..3927
                     /number=13
                     /gene="IDUA"
                     /evidence="EXPERIMENTAL"
                     /citation=[1]
     intron          3928..4074
                     /number=13
                     /gene="IDUA"
                     /evidence="EXPERIMENTAL"
                     /citation=[1]
     exon            4075..>4480
                     /number=14
                     /gene="IDUA"
                     /citation=[1]
                     /note="putative"
     intron          join(M95739:1448..1758,1..426)
                     /number=2
                     /cons_splice=(5'site:no,3'site:yes)
                     /gene="IDUA"
                     /evidence="EXPERIMENTAL"
                     /citation=[1]
                     /note="Intron 2 is about 13 kilobases long. The 5' end of
                     this intron is reported in another sequence containing
                     exons I and II of alpha-L-iduronidase"
     CDS             join(M95739:583..740,M95739:1307..1447,427..512,697..804,
                     1283..1378,1494..1696,1797..1976,2084..2300,2547..2759,
                     2851..2972,3160..3285,3364..3440,3827..3927,4075..4208)
                     /gene="IDUA"
                     /product="alpha-L-iduronidase"
                     /codon_start=1
                     /translation="MRPLRPRAALLALLASLLAAPPVAPAEAPHLVQVDAARALWPLR
                     RFWRSTGFCPPLPHSQADQYVLSWDQQLNLAYVGAVPHRGIKQVRTHWLLELVTTRGS
                     TGRGLSYNFTHLDGYLDLLRENQLLPGFELMGSASGHFTDFEDKQQVFEWKDLVSSLA
                     RRYIGRYGLAHVSKWNFETWNEPDHHDFDNVSMTMQGFLNYYDACSEGLRAASPALRL
                     GGPGDSFHTPPRSPLSWGLLRHCHDGTNFFTGEAGVRLDYISLHRKGARSSISILEQE
                     KVVAQQIRQLFPKFADTPIYNDEADPLVGWSLPQPWRADVTYAAMVVKVIAQHQNLLL
                     ANTTSAFPYALLSNDNAFLSYHPHPFAQRTLTARFQVNNTRPPHVQLLRKPVLTAMGL
                     LALLDEEQLWAEVSQAGTVLDSNHTVGVLASAHRPQGPADAWRAAVLIYASDDTRAHP
                     NRSVAVTLRLRGVPPGPGLVYVTRYLDNGLCSPDGEWRRLGRPVFPTAEQFRRMRAAE
                     DPVAAAPRPLPAGGRLTLRPALRLPSLLLVHVCARPEKPPGQVTRLRALPLTQGQLVL
                     VWSDEHVGSKCLWTYEIQFSQDGKAYTPVSRKPSTFNLFVFSPDTGAVSGSYRVRTLD
                     YWARPGPFSDPVPYLEVPVPRGPPSPGNP"
BASE COUNT      619 a   1569 c   1516 g    776 t
ORIGIN      
        1 tcctgtgcac tcatgttgcc tcttggggtg tgggagggga aatggggcac tcctgggcct
       61 ccaggaggtg cagagaacca gggtgaggtg tccaccaggt cctgcctggc tcctgacccc
      121 tggcccctgc tgctcgcgac tggcctgcct cgtgccactg agcctcagag ccattccgaa
      181 cccccacccc aagttttcca tctcttgatg gtgtagggtt ggggggtctc catgtacaga
      241 tactctagtt cataccaggc cttcataggg ttattttcca aggggaaggg cccctcggga
      301 agccgggatc ggagtcctgt gtggcacctt gcaggctccc acatgctccg ttgtggccac
      361 ggttccagcc tggagcatgg agctgtgtgg gcaccctgct tcctgacgct gaccgtcctt
      421 ctgcaggggg tccactggac ggggcctgag ctacaacttc acccacctgg acgggtacct
      481 ggaccttctc agggagaacc agctcctccc aggtgagctg tgggctctgc cctcccagcc
      541 cgcctgcacc cccttgccct gcccaccctc tccctcaccc agcccctctg agtccttgga
      601 tgtccattca gggctggcct tggtgccgga gcacaggcct ggcagagcat gggtgtggtg
      661 tgtggtgggc ggtggggcag ccctcctgtg ttccagggtt tgagctgatg ggcagcgcct
      721 cgggccactt cactgacttt gaggacaagc agcaggtgtt tgagtggaag gacttggtct
      781 ccagcctggc caggagatac atcggtgggc gagcgcaggc cctggggccc tggccggggc
      841 gggggtactc ctgggcaggt tgcaccccta tcacgcaggc tgctgcctgg tcaggagata
      901 cattggtggg caggcgcagg cccttgtggg gggatggggg tgacaaggga taggttggtg
      961 gtcggcgcag gccctggggc cccaggctgg ggggtactcc tgggcttggt gggtgggcga
     1021 aggccctggg cccctggggt ggggggtact cctgggcagg ctgcacccct atcacccagg
     1081 ccgcacccct atcacccagg ccgccgccca ggtcttggac ccccttgagc cagcgcttcc
     1141 tgatgtgggg cgggaggctg gcctgcatgg agatggggtt catcttgagt cagacgccct
     1201 tcatcacctt gcaccctccc tccgtgggag tcactgaggc gagattcacc tgtgctgggg
     1261 ggacagcaag gctcctctgc aggtaggtac ggactggcgc atgtttccaa gtggaacttc
     1321 gagacgtgga atgagccaga ccaccacgac tttgacaacg tctccatgac catgcaaggt
     1381 gtgcaccgct tcctggggtc ctgcccggct gaaagggggc agaggaaggc aggagcagag
     1441 gctaagccgc tcatccccag ggcaggtgta gacgcagtgc tcccccggcc caggcttcct
     1501 gaactactac gatgcctgct cggagggtct gcgcgccgcc agccccgccc tgcggctggg
     1561 aggccccggc gactccttcc acaccccacc gcgatccccg ctgagctggg gcctcctgcg
     1621 ccactgccac gacggtacca acttcttcac tggggaggcg ggcgtgcggc tggactacat
     1681 ctccctccac aggaaggtgc gccctgcccc tccgtccgcc ccggtgttct gcgccctcag
     1741 ccgctgtgcc ccgggccgcg ctgaccctgg tggtgctgag gcggccccgc ccgcagggtg
     1801 cgcgcagctc catctccatc ctggagcagg agaaggtcgt cgcgcagcag atccggcagc
     1861 tcttccccaa gttcgcggac acccccattt acaacgacga ggcggacccg ctggtgggct
     1921 ggtccctgcc acagccgtgg agggcggacg tgacctacgc ggccatggtg gtgaaggtgg
     1981 gccggcccaa cgccctgcgc gccccccggc caccttcctc ccgagacggg acaggcgagc
     2041 ggtggccgcg ccacccggtc ccagctgccc tggacacccg caggtcatcg cgcagcatca
     2101 gaacctgcta ctggccaaca ccacctccgc cttcccctac gcgctcctga gcaacgacaa
     2161 tgccttcctg agctaccacc cgcacccctt cgcgcagcgc acgctcaccg cgcgcttcca
     2221 ggtcaacaac acccgcccgc cgcacgtgca gctgttgcgc aagccggtgc tcacggccat
     2281 ggggctgctg gcgctgctgg gtgagccggg gccgctgggg tgggccggcc agggccctcc
     2341 aggctgggga gcggctcctg cgaaggcccc gctgcgggga gcgcacttcc tccagccgcg
     2401 cgcttcccgg ggtcggcctc cgcgtggcgg ggcctgggga ctccttcacc aaggggaggg
     2461 ggagcgagtg gtgggaggcc cggccctggg tcggggggcg gctgggcaac gaccccacgc
     2521 ggcgacggcc cccccccgcc ccgcagatga ggagcagctc tgggccgaag tgtcgcaggc
     2581 cgggaccgtc ctggacagca accacacggt gggcgtcctg gccagcgccc accgccccca
     2641 gggcccggcc gacgcctggc gcgccgcggt gctgatctac gcgagcgacg acacccgcgc
     2701 ccaccccaac cgcagcgtcg cggtgaccct gcggctgcgc ggggtgcccc ccggcccggg
     2761 taagccgggg ttccagggag gtctctggcc ccgctggggc tctggagggg gcggcccggg
     2821 gagccgaggc ctgagtgtca ggccccgcag gcctggtcta cgtcacgcgc tacctggaca
     2881 acgggctctg cagccccgac ggcgagtggc ggcgcctggg ccggcccgtc ttccccacgg
     2941 cagagcagtt ccggcgcatg cgcgcggctg aggtaggtgg gccgcggagg ggcgaggggc
     3001 cgggccgggc cggggtcccg ggggggtggg gtccggggcg ggggctccga ggcggtgtgg
     3061 gtgggaggtg gagcggtggg ccgggggcgt tcgccctgag gtcgggccga gcgtccccag
     3121 ctcccctgga gaaccctgag gaccggccac tgcgcccagg acccggtggc cgcggcgccc
     3181 cgccccttac ccgccggcgg ccgcctgacc ctgcgccccg cgctgcggct gccgtcgctt
     3241 ttgctggtgc acgtgtgtgc gcgccccgag aagccgcccg ggcaggcaag tggcagtccc
     3301 ctaacccgcg ccgcggcccg gactcccctt ccccgacgcc atcacagccc ttccctcccc
     3361 caggtcacgc ggctccgcgc cctgcccctg acccaagggc agctggttct ggtctggtcg
     3421 gatgaacacg tgggctccaa gtgcgtgagt ggggccgccc ctccctctgc ctggtcctag
     3481 gcaggtccct gggtcccgac cccttcaccc atgcggtcac tcgggccact tgccgtggcc
     3541 catcggctcc ctccctcgcc gccctgcgtc cctgcccttc accccacaca ctgtgggcca
     3601 cgcgccaggc cctgccagtg gggtgtgggt tctcctaggg gacatgagat ggacattcgg
     3661 gctccagccc tctcctgcct gggcaggaag agtgcccagg ggctggggag gtgccgccga
     3721 ggggcttgag ggaatgaggc tgtgggtcca cgcggccgtg ccctgcctgc tcccaccttt
     3781 gaggactgtc ttgaccccag ccttgttctt ggcctgacct ccccaggtgc ctgtggacat
     3841 acgagatcca gttctctcag gacggtaagg cgtacacccc ggtcagcagg aagccatcga
     3901 ccttcaacct ctttgtgttc agcccaggtg cgcccaccac ccgctgccct ggactcggcc
     3961 accccattct tgggcctcag ggcagtactg ggtgggggcc tcgagaagcc tggggtcagg
     4021 gggctttcgg gtgggggcag gttccggttg gcacacatgt ccccttgtct ccagacacag
     4081 gtgctgtctc tggctcctac cgagttcgga ccctggacta ctgggcccga ccaggcccct
     4141 tctcggaccc tgtgccgtac ctggaggtcc ctgtgccaag agggccccca tccccgggca
     4201 atccatgagc ctgtgctgag ccccagtggg ttgcacctcc accggcagtc agcgagctgg
     4261 ggctgcactg tgcccatgct gccctcccat cacccccttt gcaatatatt tttatatttt
     4321 attattttct tttatatctt ggtaccaacg ccccctttaa agcggctttg cacaggtcag
     4381 tctcgggttg aggctctgtg gcttggccct gggcacattc cagggcagcc tccaagggta
     4441 aaccccggtg gctgatgagg acccagctgg agcgaggcct
//