From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: D.pseudoobscura Adh gene for alcohol dehydrogenase
Date: 28 Dec 92 21:27:43 GMT
Distribution: bionet
Checksum: 56436 8

LOCUS       DROADHGN62   3493 bp ds-DNA             INV       01-DEC-1992
DEFINITION  D.pseudoobscura Adh gene for alcohol dehydrogenase
ACCESSION   X64471
KEYWORDS    ADH gene; alcohol dehydrogenase.
SOURCE      Drosophila pseudoobscura DNA.
  ORGANISM  Drosophila pseudoobscura
            Eukaryota; Animalia; Metazoa; Arthropoda; Uniramia; Insecta;
            Pterygota; Neoptera; Holometabola; Diptera; Brachycera;
            Cyclorrhapha; Schizophora; Drosophiloidea; Drosophilidae.
REFERENCE   1  (bases 1 to 3493)
  AUTHORS   Schaeffer,S.W. and Miller,E.L.
  TITLE     Estimates of gene flow in Drosophila pseudoobscura determined from
            nucleotide sequence analysis of the alcohol dehydrogenase region
  JOURNAL   Genetics 132, 471-480 (1992)
  STANDARD  full automatic
COMMENT     *source: strain=PS192; *source: isolate=Stemwinder Provincial Park,
            B.C. Canada; *source: chromosome=IV section 88;
               From EMBL    entry DPADHGN62;  dated 16-OCT-1992.
FEATURES             Location/Qualifiers
     mRNA            join(40..119,917..1022,1086..1490,1551..1966)
                     /note="adult mRNA (Adh)"
     mRNA            join(884..1022,1086..1490,1551..1966)
                     /note="larval mRNA (Adh)"
     promoter        8..14
                     /note="adult promoter (Adh)"
     prim_transcript 40..1966
                     /note="adult primary transcript (Adh)"
     exon            40..119
                     /note="adult exon 1a"
     intron          120..916
                     /note="adult intron"
     prim_transcript 884..1966
                     /note="larval primary transcript (Adh)"
     exon            884..1022
                     /note="larval exon 1"
     exon            917..1022
                     /note="adult exon 1b"
     intron          1023..1085
                     /number=1
     exon            1086..1490
                     /number=2
     intron          1491..1550
                     /number=2
     exon            1551..1966
                     /number=3
     polyA_signal    1943..1948
     polyA_site      1966
     CDS             join(930..1022,1086..1490,1551..1817)
                     /EC_number="1.1.1.1"
                     /gene="Adh"
                     /product="alcoholdehydrogenase"
                     /codon_start=1
                     /translation="MSLTNKNVVFVAGLGGIGLDTSRELVKRNLKNLVILDRIDNPAA
                     IAELKAINPKVTITFYPYDVTVPVAETTKLLKTIFAQVKTIDVLINGAGILDDHQIER
                     TIAVNYTGLVNTTTAILDFWDKRKGGPGGIICNIGSVTGFNAIYQVPVYSGSKAAVVN
                     FTSSLAKLAPITGVTAYTVNPGITKTTLVHKFNSWLDVEPRVAEKLLEHPTQTSQQCA
                     ENFVKAIELNKNGAIWKLDLGTLEPITWTQHWDSGI"
BASE COUNT      973 a    796 c    802 g    922 t
ORIGIN      
        1 cgtagtgtat ttaaggcact gcacatgtcg aggaccacga ttattgtcta cagtgcagtc
       61 agtagttgtc aggtgcagca ggcggaccag catttcttta aatctactca atcgatcaag
      121 taagtaactg tgtaatccaa gtacagtcgg cccatcccac tcggttctgc cgtaactcaa
      181 gaaatttctt gatagcgcag ttccagttgt aaaacgtcgt ctaaaaaaac atctcgttgg
      241 agcttgggtt ggaaaacgtt cgagttgggc gtagaaaagt tcaaatataa aatatcaaaa
      301 aagagaaaat atcttcccaa agcagctgtc cattttttgt taaagtgata gatcaaaaat
      361 tcgtgtgacg tacattccta gtgcacttca tacaaataat ttcctcaatt ctaattctac
      421 aagaagcact tttggctctt gtccagaaaa aacaatttgt ttacacttgt cggatgataa
      481 cataagcgga ccgacggcta agtaacataa cagtcccctg ttaagtaatg ccctcactct
      541 ctgttagatg gccgcgcgtg tcgactccga tagaccagca gacacgcagt agttttcctc
      601 aagaacctga actctaaaca tagacataat ttacgatacc ttgagactca aataaaaata
      661 caagtatgag tggtattcta ttcctgatta gactcgcaca cacacacgta cgagtattaa
      721 cataaatttg ccggcaaata accaaaacaa aacgaacgca aacgatccga acacgtaatg
      781 cgagagataa gaaacagaaa agctcgacgt gaaagaagct tctggcggaa gctcttgccg
      841 aactccctat aaatacgaga cagaaaacca gaataaatat aataacaaac tgttgaattg
      901 tcccatcgat tcccagatca aaagacagaa tgtctctcac caacaagaac gtcgttttcg
      961 tggccggtct gggcggcatt ggcctggaca ccagccggga gttggtcaag cgtaatctga
     1021 aggtaagagt gaacgaattc catggaatct atggaatcct aaatttaaaa attcatttat
     1081 tgtagaacct ggtcatcctg gatcgcattg acaatccggc tgccattgcc gaactgaagg
     1141 caatcaatcc caaggtgacc atcaccttct atccctacga tgtgactgtg cccgtcgctg
     1201 agaccaccaa gctcctgaag accatctttg cccaggtgaa gacaatcgat gtcctgatca
     1261 acggtgctgg catcctggac gatcatcaga tcgagcgcac cattgccgtt aactacacgg
     1321 gcctggtcaa caccaccaca gccattctgg acttctggga caagcgcaag ggcggcccag
     1381 gcggcatcat ttgcaacatt ggctccgtca ccggtttcaa tgccatctac caggtgcccg
     1441 tttactccgg ctccaaggcg gcggtggtta actttacctc ctccctggcg gtaagtaatc
     1501 caacgttttt tctactaccc aaaactaaat atttctttga aatcccatag aaactggctc
     1561 ccattactgg tgtcactgct tacactgtca atcctggcat cactaagacc actctggtcc
     1621 acaaattcaa ctcgtggctg gatgtggagc cccgtgtggc agagaagctg ctcgagcatc
     1681 ccacccagac ctctcagcag tgcgccgaga actttgtgaa ggccattgag ctgaacaaga
     1741 acggtgccat ctggaagttg gatctgggca ccttggagcc catcacatgg acccagcact
     1801 gggactcggg catctaaacc atcccagaga ctctatggga catggcgttt tagcttttag
     1861 tttctttttt tccactcaat tgttacgtat atacatacac atatggcaat aaggctgatt
     1921 tgaaccccgt tttgaatatg ataatacaaa attatatttg agaaatttca acaaaatcga
     1981 taagagctat agctctcttt ttttttaatt aaaaaaaact agataaaaag agcaatggtc
     2041 agtggtggtc agtggtcgtt gtggcctcta tcgatttcca cacaaaaact ttactgttta
     2101 gtaatagaaa agaacttgag aaaggcagcc aaaagaatgt acgatctgac gggtaagcat
     2161 gtctgctatg tggctgactg cggtggcatt gcactggaga ccagcaaggt tctcatgacc
     2221 aagaatatag cggtgagtgg tagagtggtg gtgtagagag tggagagtgt tccgaagtgg
     2281 ttctggttct taaacaagtc tctagtctct agtctctagt ctcttggctg ctgctggtag
     2341 ctgtcgaaac taattaagtt aatgactttt tcatgttttg tttttgtccg ctgacttttg
     2401 ctgttcaaag ctacaattaa gtttatgttt tgagtttgat ctacttttgc ttaagggaag
     2461 tactttcgat tacggattgc tggcatataa aaatagatac caaggaatgt gcatcgtatc
     2521 atattgattc tatcttgatt ctttgcccag aaactggcca tccttcagag cgtggagaac
     2581 cccccggcca tcgctcagtt acaatcgatt aagcacagca cacagatctt cttctggacc
     2641 ttcgatgtga ccatggcccg cgaggagatg aagaagtact tcgatgaggt catggtccag
     2701 atggactaca tcgatgtgct gatcaatggg gccaccctgt gcgatgagcg gaacattgat
     2761 gccaccatca atacgaatct gaccggcatg atgaacaccg tggccactgt gctgccctat
     2821 atggaccgaa agatgggcgg ttcgggtgga ctgattgtga atgtcacctc cgtcatagga
     2881 ctggatccat cgccagtctt ttgtgcgtac agtgcctcaa agtttggtgt gattgggttc
     2941 acccgaagtc tagcggtgag tgggcgagat cttaacttga aaatcctctc taatgtcttc
     3001 cattctttcg caggatcccc tgtattacac ccaaaatggt gtggctgtca tggccgtctg
     3061 ctgtggtccc accaaagtgt ttgtcgatcg ggaactgact gccttcctgg aatacggcca
     3121 gtcctttgcc gatcgcctgc gtcgtgcccc ctgccaatcg acggccgtct gcggccagaa
     3181 tatagtaaat gccatcgaga gatcggagaa cggacagatc tggattgccg acaagggcgg
     3241 cctggagtcg gtggccctcc actggtattg gcatatggcc gatcagtttg tgaactatat
     3301 gcagagcact gacgatgagg atcaggagtt ttttttgggg cagcgatgag gcatgccttg
     3361 atccacggaa taaaacatca aatgtgtcac aaaaagtagg caacttgttt gtgggtctgg
     3421 aaatagattt gaaatatttt gaggtcatat gaataatgac ataaatgagg gtgcctgaaa
     3481 gtatgccaag ttt
//