From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Drosophila melanogaster topoisomerase I mRNA, complete cds.
Date: 29 Dec 92 19:57:13 GMT
Distribution: bionet
Checksum: 37450 9

LOCUS       DROTOPISO    5132 bp ss-mRNA            INV       10-DEC-1992
DEFINITION  Drosophila melanogaster topoisomerase I mRNA, complete cds.
ACCESSION   M74557
KEYWORDS    topoisomerase I.
SOURCE      Drosophila melanogaster (library: lambda gt11) cDNA to mRNA.
  ORGANISM  Drosophila melanogaster
            Eukaryota; Animalia; Metazoa; Arthropoda; Uniramia; Insecta;
            Pterygota; Neoptera; Holometabola; Diptera; Brachycera;
            Cyclorrhapha; Schizophora; Drosophiloidea; Drosophilidae.
REFERENCE   1  (bases 1 to 5132)
  AUTHORS   Hsieh,T.-s., Brown,S.D., Fostel,J. and Huang,P.
  TITLE     Isolation and Characterization of a Gene Encoding DNA Topoisomerase
            I in Drosophila melanogaster
  JOURNAL   Nucleic Acids Res. 20, 6177-6182 (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     CDS             259..3177
                     /evidence="EXPERIMENTAL"
                     /product="topoisomerase I"
                     /codon_start=1
                     /translation="MSGDVAAENSIHIQNGGSCEVVQSNGVTTNGHGHHHHHHSSSSS
                     SSKHKSSSKDKHRDREREHKSSNSSSSSKEHKSSSRDKDRHKSSSSSSKHRDKDKERD
                     GSSNSHRSGSSSSHKDKDGSSSSKHKSSSGHHKRSSKDKERRDKDKDRGSSSSSRHKS
                     SSSSRDKERSSSSHKSSSSSSSSKSKHSSSRHSSSSSSKDHPSYDGVFVKPEPVSQQL
                     MHSGSVDAFQMQQLGSYEAAAAGTNFNGNGNVAGANYKNGYEESIVDIKKEEESFNNL
                     SQASSCDYSMSQFRADEPPFVVKHEQSYAEEDSTMNYNDHDDDADEMNDDEEDVPLAM
                     RKRKQEATDRPDGGMDNDDDDDIPLLARKKVKKEKIKKESKEKSKKRVKEEPSDDYGN
                     VKPKKKKMKKEPEPAVSPGKRQKAKAKVEEEEVWRWWEEEKRADGVKWSTLEHKGPVF
                     APRYERVPRNVRFYYDGKPLELSEETEEAATFYAKMLNHDYCTKEVFNNNFFKDFRKS
                     MTPREREIIKDFRKCNFQEMFNYFQAESEKRKAASKEEKLIKKNENEALMKEFGFCMI
                     DGHKEKIGNFRLEPPGLFRGRGEHPKMGMIKRRIQASDVSINCGKDSKVPSPPPGSRW
                     KEVRHDNTVTWLASWIENVQGQVKYIMLNPSSKLKGEKDHIKYETARRLDKVIDKIRA
                     TYRDEWKSKEMRVRQRAVALYFIDKLALRAGNEKDEDQADTVGCCSLRVEHVQLHKEL
                     NGKENVVVFDFPGKDSIRYYNEVEVEKRVFKNLELFMEHKKEGDDLFDRLNTQVLNEH
                     LKELMEGLTAKVFRTYNASKTLQSQLDLLTDPSATVPEKLLAYNRANRAVAILCNHQR
                     SVPKSHEKSMENLKEKIKAKREAIEKCESEYHSRDEKKGKQLERLRDQLKKLELQETD
                     RDENKTIALGTSKLNYLDPRISVAWCKKHDVPIEKIFNKTQRTKFLWAVHMADENYRF
                     "
BASE COUNT     1660 a   1209 c   1201 g   1062 t
ORIGIN      
        1 gcgtggctgc tgcgttccgt aaaacgcaaa gaagccaaaa agcaagggca ttcgcattga
       61 ccaaagcacc aaacgctgct ccttttcgaa aaacttcagt gcgcttttat ttccccgccg
      121 ctgtcaccgc tttaaggagc cccaacatct aattattgaa aatcctaacg aacagtttag
      181 tcgaacagtg taaaagcgtg aaaagaaaag aaaggaacga ataaaataaa agaaaaaccg
      241 ctaagcgagt tccgcaaaat gagtggggat gtggctgccg aaaatagcat ccacatacaa
      301 aatggtggca gctgcgaggt ggttcaatcc aacggggtga ccaccaatgg acacggacac
      361 caccatcatc accacagcag cagcagcagc agcagcaagc ataaatcctc cagcaaggac
      421 aagcatcgtg atagggagag ggagcacaag agctccaatt cctccagctc gtcgaaggag
      481 cacaagagca gcagtcgcga caaggatcga cacaagagca gcagttcgtc gtcaaagcat
      541 cgggacaagg acaaggagcg cgatggcagc agcaactcgc atcgcagcgg ctcatcgtcc
      601 agccacaagg ataaggatgg gagcagcagc agcaagcaca agtcgtcgtc gggccaccac
      661 aagaggtcct cgaaggataa ggagcgacgc gataaggaca aggatcgcgg cagcagttca
      721 tccagtcggc acaagtcctc gtccagctcc cgtgacaaag agcgcagcag cagcagtcac
      781 aagagcagct cctcttcatc ctcatcgaag agcaaacatt ccagttcgcg gcacagcagc
      841 tcatcttcct cgaaagacca tccatcgtac gatggcgtat ttgttaagcc ggagcccgtt
      901 tcccaacagc tgatgcactc gggctcggtg gatgctttcc agatgcaaca gctcggtagc
      961 tacgaagccg cagccgccgg caccaatttt aatggcaatg gaaacgttgc cggtgccaac
     1021 tataagaacg gctacgagga atcgatcgta gacatcaaga aggaggagga gagctttaac
     1081 aatctgtcgc aagccagctc ctgtgactac tccatgtccc agtttcgtgc cgatgagccg
     1141 ccgtttgtgg tgaagcacga gcagagctac gccgaagagg acagcaccat gaactacaat
     1201 gatcacgacg acgacgccga cgagatgaac gacgacgagg aggatgtgcc gctggccatg
     1261 cgtaagcgca aacaggaggc aaccgatcgt cctgatggcg gcatggataa cgacgatgac
     1321 gacgacattc cgctgctggc gcgcaagaag gttaagaagg agaagatcaa gaaggagtcc
     1381 aaggaaaagt ccaagaagcg ggtcaaggag gagcccagcg atgattacgg caatgtcaag
     1441 ccgaaaaaga aaaagatgaa aaaggagccc gagcctgccg tatcccccgg caaacgacag
     1501 aaggcaaagg ccaaggtgga ggaggaggag gtttggcgat ggtgggagga ggagaagcgt
     1561 gccgatggcg tcaaatggtc aacgctggag cacaagggac ccgtgtttgc accgcgatac
     1621 gaacgggtgc cacgcaatgt acggttctac tacgatggca agccgttgga gctctctgag
     1681 gaaacggagg aggcagccac gttctatgcc aagatgctga accacgacta ctgcaccaag
     1741 gaagtgttca ataacaactt cttcaaggac ttccgcaagt ccatgacgcc cagagagagg
     1801 gagattatca aggatttccg caagtgcaac ttccaggaga tgttcaacta cttccaagcg
     1861 gagtccgaaa agcgcaaggc agccagcaag gaggagaagc tgatcaagaa gaacgaaaac
     1921 gaggcgctga tgaaggaatt cggattctgc atgattgatg ggcacaagga gaagatcggt
     1981 aacttccgcc tggagccgcc aggcctgttc cgcggtcgtg gcgagcatcc caaaatgggc
     2041 atgatcaagc ggcgcatcca agccagcgat gtgtccatta actgtggcaa agattcaaag
     2101 gtgccttcac cgccgcccgg atctcgctgg aaggaggtgc gccacgacaa cacggtcacc
     2161 tggctggcct cctggatcga gaatgtgcag ggacaggtca agtacatcat gttgaatccc
     2221 tcctcgaaac tcaagggcga gaaggatcac attaagtacg agacggcgcg acgcctggac
     2281 aaggtaatcg ataagatacg tgccacctat cgcgacgagt ggaagtccaa ggagatgagg
     2341 gttcgccagc gagcggtggc cctttacttc atcgacaaac tggcgctgag agcaggcaac
     2401 gaaaaggacg aggatcaggc cgataccgtg ggctgttgct cgctacgcgt ggagcatgtc
     2461 cagctccaca aggaactgaa cggaaaggag aacgtggtgg tctttgattt ccccggtaag
     2521 gactccattc ggtattacaa cgaggtcgag gtggagaagc gtgtcttcaa gaacctcgaa
     2581 ctgttcatgg agcacaagaa ggagggcgac gacctcttcg atcgtctcaa cacgcaggtg
     2641 cttaatgagc atctcaagga gctgatggaa ggtctcacgg ccaaggtatt ccgtacgtac
     2701 aacgcatcga aaacactgca aagtcagctg gatctgttga ccgatccgag cgccacggtg
     2761 ccggaaaagc tgttggccta caatcgcgcc aaccgtgccg tggccatcct ctgtaaccat
     2821 cagcgttccg tgcccaagag ccacgagaag tcaatggaga atctgaagga gaagatcaag
     2881 gccaagcggg aagccatcga aaaatgcgag tccgagtatc attcgagaga cgagaagaag
     2941 ggcaaacagc tggagcgcct aagggatcag ctgaagaaac tagaactaca agagactgat
     3001 agagacgaga ataagaccat tgccctgggc acatctaagc taaactatct tgatccacgc
     3061 atatcggtgg catggtgtaa aaagcatgac gtgccgatcg agaagatatt caacaaaacg
     3121 caaagaacca aatttctgtg ggccgttcat atggccgatg agaattatcg tttctaaatg
     3181 ggttctcctc acagccacgc ctagacacaa acacacaagt acgccgaccc ttcctccacc
     3241 caccaccacc ccttcagaca cacacacaaa cacacactcg cacccgatcg aagaagatgt
     3301 aacaacaaac cacacccacc acaaggccgg tttataaata gattccatgc caatcccgcc
     3361 attgtttggt ccacagttta tgcaattgat agtgcatatg tccagctttc ggccaacgca
     3421 atgggggatt gttagccaaa atgagcggga cggaatgctg cactacatcg tagtcgtgga
     3481 accgcttgta cgcgtccgtt ttgtctgctt tgaatgggat taatgggatc aagtgcatac
     3541 tacgccgaag tctaacttgc gaaatgtgca cacccaaaca caagctaatc caatatatgt
     3601 cagttttatt cattaaaggc aagaggagat gtgaaacctg ggcaagacgt cgccgccacc
     3661 taaaaacaaa tcaggagcat aattttctat gtctctacaa aagaactttt ttgtcaagat
     3721 acccttatac ttcataattt tacatataaa acatatatac atatatatat ttatatttat
     3781 gtatataata atatttgtgt atatattttt atttttaata taatacatac atttacataa
     3841 atatatatat atagatatat atatatataa aaatatagat gtatatatat atatatatat
     3901 atttaatagc aaaagcaaac gtaaaattat acaatttaaa acgcaatttt ttttatgttt
     3961 acctatagtt gattttgttt agcgaaaaat gtctttcact attaattaat attaatatta
     4021 cgattactgc gtatttgtat aagtgcaaac tgaacagaga aaccttgtag cccaactttt
     4081 agcatagcat aatttattca attcaactct gaagcagcgc tatttgtcga ccgaccgccc
     4141 ccgaaaatga agaacaaagc cacattggat ggatctcgat gtggtttcgc tttgatttcg
     4201 taccttatga tcattttagt taacatttcg aaatatacaa atgctaaatt gatttgcaga
     4261 cagaccctta actttctata ctctaagcag acaggtttca agttctcaac cgcaaaccaa
     4321 gccgccatca cactgaaaac caattgcaga tacaaatcaa acgagcaatt caaataactc
     4381 gatgaaccaa aacagaaaca cagccagcaa accagtcgca gcgaaagaaa gaactttaat
     4441 ctgaaagaaa ggaactttat acatatagaa tctatagaag tatgtgcaat taagtgagct
     4501 aaaactatac cttaaacctt gtaaaaatcg agccttcact tgaatccttc agtcgacgag
     4561 agcagtggat tcaggtggag ttaggaacat ccccaacatc catgtgcccc aatcccatca
     4621 gctacgatta tggccgtact tttaactaag caaatcgatg gaaatttata tctcgcctca
     4681 cgccccccct agcataaatg gcaaacgcgc attgcaaatg aagagcgaac gatctttata
     4741 tacatacata taaacacaca cacacacacg cccaaacaca tattataatt agtgattgat
     4801 ataatgtgaa cgcaaacatc tgtgtgcgtt tgtcgtggat tcgcatacgg aagcgggaga
     4861 atgcttttaa aaggcaaaaa aaaaagttaa ataaactaat tacgaaccct tcttcgaacg
     4921 cccagccccg ccccgccccg ccacaacact cacatataaa cttggcatat tataatattg
     4981 atataagcaa acaaaaaaca acaagcgaaa tgataagaaa cccactattt taaatcgaaa
     5041 gcaaagtaaa tgaaaaacaa aatagcataa gtgaatgtaa ttgcaatgga atgatgcaaa
     5101 tatacataaa atatatattt aattttaaaa tc
//