From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: W.succinogenes genes psrA, psrB and psrC for polysulphide reductase
Date: 10 Apr 92 10:14:27 GMT
Distribution: bionet
Checksum: 44318    10

LOCUS       WOLPCRABC    4946 bp ds-DNA             BCT       10-APR-1992
DEFINITION  W.succinogenes genes psrA, psrB and psrC for polysulphide reductase
ACCESSION   X65042
KEYWORDS    polysulphide reductase; psrA gene; psrB gene; psrC gene.
SOURCE      Wolinella succinogenes DNA.
  ORGANISM  Wolinella succinogenes
            Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
            Anaerobic gram-negative straight, curved and helical rods.
REFERENCE   1  (bases 1 to 4946)
  AUTHORS   Krafft,T., Bakranz,M., Klimmek,O., Schroeder,L., Fahrenholz,F.,
            Kojro,E. and Kroeger,A.
  TITLE     Cloning and nucleotide sequuence of the psrA gene of wolinella
            succinogenes polusulphide reductase
  JOURNAL   Eur. J. Biochem. (1992) In press
  STANDARD  full automatic
COMMENT     From EMBL    entry WSPCRABC;  dated 27-MAR-1992.
FEATURES             Location/Qualifiers
     -35_signal      463..468
     -10_signal      491..496
     RBS             542..548
                     /gene="psrA"
     CDS             556..2847
                     /note="gtg start codon"
                     /gene="psrA"
                     /translation="METTMTRRDFLKSAGAAGAAGLVWSQTIPGTLGALEKQEIKGSA
                     KFVPSICEMCTSSCTIEARVEGDKGVFIRGNPKDKSRGGKVCARGGSGFNQLYDPQRL
                     VKPIMRVGERGEGKWKEVSWDEAYTFIAKKLDEIKQKHGAHTVAFTARSGWNKTWFHH
                     LAQAYGSPNIFGHESTCPLAYNMAGRDVFGGSMNRDFAKAKYIINMGHNVFEGIVISY
                     VRQYMEAIENGAKVVTLEPRLSVMAQKASEWHAIKPGHDLPFVLGFMHTLIFENLYDK
                     KFVQKYCTGFEELKASIEPCTPEKMALECDIPADTIKRLAREFAKAAPKAIFDFGHRV
                     TFTPQELELRRAMMMVNALVGNIERDGGMYFGKNASFYNQFLGEEDPKAKGLKKPKTP
                     AYPKVEVPRIDRIGEKDGEFFLANKGEGIVSLVPKATLNELPGVPCKIHGWFIVRNNP
                     VMTQTNADTVIKALKSMDLVVCVDIQVSDTAWFADVVLPDTTYLERDEEFTAGGGKNP
                     SFGIGRQKVVEPLGDAKPGWKIAKELSEKMGLGEYFPWKDIEDYRLQQVDGDLDLLAK
                     LKKDGSASFGVPLMLQEKKSVAEFVKKFPGAASKVNEEGLIDFPKKIQLFSPKLEEVS
                     GKGGLGYEPFKYKEEDELYFVQGKTPVRSNSHTGNVPWLNNLMEYDAIWIHPKTASKL
                     GIKNGDAIELYNKFSSQKSKALITEGVREDTLFGYFGFGHVSKDLKRAYGKGVNSNAL
                     MPSFTSPNSGMDLHVFGVKVKKA"
     RBS             2850..2854
                     /gene="psrB"
     CDS             2863..3438
                     /gene="psrB"
                     /translation="MAKKYGMIHDENLCIGCQACNIACRSENKIPDSVYRLQVWVQGP
                     KKLPDGTLSFNYHRQSCVQCENTPCVSVCPTKASYVNEDGIVSVNVDLCVGCLYCIAA
                     CPYQARYVDPVTKAPDKCNFCKDTRLARGEEPACVTVCPTDALTFGDMSDPKSKINKV
                     LASKPTLRPKESLGTKPKLFIVPNKRGGIES"
     RBS             3420..3425
                     /gene="psrC"
     CDS             3435..4388
                     /gene="psrC"
                     /translation="MNQMWGSIEQYNTVVWHWPIAVYLFLAGLSAGAIISAIIIKWMK
                     GNESSPWDGIIKAGALIAPLTIGAGLLLLIFDLTRPLHFWKLLIFYNFSSVMTLGVLA
                     LFAYFPVVLIFLLGVFKKELCDEGPFGFLAPLANIAYSMARPLEIVTFVLAIGVGAYT
                     GFLLSAMYSYPLLNTPILPLLFLASGISAGISGNLLIGLLFFGKSTKGENVGYLHGLD
                     FKVILFEAFLLFILFVGMYYQGGSTAEVAKAALTTGGLASLFWLGVAGMGLALPVVLN
                     VALPHGIKHSSGFVMLNALIVLAGVMALRFYILYAGQTFVG"
BASE COUNT     1304 a   1116 c   1270 g   1256 t
ORIGIN
        1 agatcttgac atagcgaccc ccctcgctct tctggacaaa gaaagatcgg ctaagaaggg
       61 tgtggtagcg gaggagattt tctagcgcca cagaaatcgt gggagagttc tctaggatgt
      121 agcctagaat ccctaaggcg ttagggcgaa tattttcccc gatatagaga cctagatttt
      181 gatttcccgt gatttttacc actcgcaaga aaatgttctt ataagaggcg atatccaccg
      241 aggcctttgc atctctgagt tggtctagaa tctcaggcga gagctcggag gaggggaata
      301 ggcgctgatt tctcgtgagg tgaccgagga tgaaattgat cgcggccgag ctaacctttt
      361 gcctcatgga ttcctttcag ttaatatcgc ctccaaagct cctgttaagg gtgttgggag
      421 ggattctcat aatggcacaa aatatcaatt tttttgaaaa ctttgtcaag aaaaaaagtc
      481 ggataaattg tatcatgaaa gcgtgactac aatcattaaa cgactgtaat ctatatcgaa
      541 taaggagggt ggcaagtgga aacaactatg acacgtagag atttcctaaa aagtgccggt
      601 gctgcgggcg cagcgggtct agtctggtcg cagacgattc ctggaacgct tggagcgctg
      661 gagaaacaag agattaaagg ctcggccaaa ttcgtcccca gtatctgtga gatgtgtaca
      721 agttcctgta cgatcgaagc aagagtggaa ggggataaag gagtctttat tcgaggcaat
      781 ccaaaagaca aaagccgtgg cggtaaagtg tgcgcgcgtg gcggctcagg ctttaatcaa
      841 ctctacgatc cccaaaggct agtgaagccc atcatgagag tgggtgagcg aggcgaaggg
      901 aagtggaaag aggtgagctg ggatgaggct tacaccttta tcgccaagaa gcttgatgag
      961 atcaagcaaa agcatggcgc tcatacggtg gctttcaccg ccagaagcgg ttggaacaag
     1021 acttggttcc atcatcttgc tcaagcctat ggctcaccta atattttcgg acacgaatca
     1081 acctgtcccc tcgcctacaa tatggcggga cgagatgtgt ttggtggcag catgaaccga
     1141 gattttgcga aggcaaaata tatcatcaac atgggtcaca atgtctttga aggaatcgtc
     1201 atctcctacg tgagacaata catggaggcg attgaaaatg gcgccaaagt ggtcacacta
     1261 gagcctagac tctctgtcat ggcacaaaaa gcgagcgaat ggcacgcgat caagccaggg
     1321 catgaccttc cctttgttct tggcttcatg cacacgctca tttttgagaa tctctatgac
     1381 aagaaatttg ttcaaaagta ttgcacaggc tttgaggagc ttaaagcctc tatcgagcct
     1441 tgcacaccag agaagatggc gctagagtgc gatattcccg ctgataccat caagcgactc
     1501 gcaagagagt ttgccaaagc agctcccaag gcgatttttg actttggtca ccgtgtcacc
     1561 ttcactcccc aagagttgga gctcagacga gcgatgatga tggtcaatgc gctagtgggt
     1621 aatatcgaga gagatggggg aatgtatttt ggcaaaaatg caagcttcta caaccaattc
     1681 ctaggtgaag aagaccccaa agccaaagga cttaaaaaac ctaaaactcc agcctacccc
     1741 aaggttgaag tgcctcgaat cgatcgcatc ggcgaaaaag atggagagtt cttcttggct
     1801 aataagggtg agggaatcgt gagtcttgtg ccaaaagcca cgctcaatga gcttcctggg
     1861 gttccctgta aaattcatgg ctggtttatc gtcagaaaca accctgtcat gactcaaacc
     1921 aacgcagaca ccgtcattaa ggcgctcaaa tcgatggatt tagtggtgtg cgtcgatatt
     1981 caggtctctg atacggcttg gtttgcggac gtggtgcttc ctgatacgac ctatcttgag
     2041 agagatgagg agttcaccgc aggtggaggc aagaatccta gctttggaat cgggcgccaa
     2101 aaagtggtcg agcccctagg agatgcaaag cctggatgga agattgccaa agagctgtct
     2161 gagaagatgg gtctgggtga atatttccca tggaaagata tcgaggatta tcgactgcag
     2221 caagttgatg gcgaccttga tcttttggcc aaactcaaaa aagatggaag cgctagcttt
     2281 ggcgtgcccc tcatgcttca agagaagaag agtgtggcag agtttgtcaa gaaattccct
     2341 ggcgctgctt ccaaggtcaa cgaagagggt ttgattgact tccctaaaaa gattcagctc
     2401 tttagtccca aactagaaga ggtttcaggg aagggagggc ttggctatga gcctttcaaa
     2461 tacaaagagg aagatgagct ctactttgtt caagggaaaa cccccgtgcg aagtaactct
     2521 cacacaggta atgtgccttg gctcaacaac ctcatggagt atgacgcgat ctggatccac
     2581 cccaaaaccg caagcaagct gggaattaaa aacggggatg cgattgagct ttataacaaa
     2641 ttcagctcgc aaaagtccaa agcgctcatc accgaagggg tgcgcgagga tacactcttt
     2701 ggatattttg gctttggtca tgtcagcaaa gacctaaaga gggcttatgg caagggagtc
     2761 aactccaatg ctctaatgcc cagcttcact tcgcctaact cagggatgga tctccatgtc
     2821 tttggcgtca aagtgaagaa agcgtaaggg gagggaagaa tcatggcaaa aaaatatgga
     2881 atgatccacg atgagaatct atgcatcggt tgccaagcgt gcaatatcgc ctgtcgatct
     2941 gaaaacaaga ttcctgatag tgtttaccga ttgcaagtct gggtgcaggg ccccaagaag
     3001 ctacctgatg gaacactcag cttcaactat caccgacaat cttgcgttca gtgtgaaaac
     3061 accccctgcg tgagtgtctg ccccaccaag gcctcttacg tgaatgaaga tgggattgtg
     3121 agcgtcaatg tcgatctttg cgttggatgc ctctattgca tcgcggcgtg tccctatcag
     3181 gcgcgatatg tggatcctgt gaccaaggct cccgataagt gcaacttctg taaagatacg
     3241 cgtctagcgc gtggcgaaga gcctgcgtgt gtgaccgtct gccctacaga tgcgcttact
     3301 tttggtgata tgagtgatcc caaaagcaaa atcaataaag tgttggcgtc caagccgacc
     3361 cttcgcccca aagaatcctt gggaactaaa cccaagctct ttatcgtgcc taacaagaga
     3421 ggaggaatcg agtcatgaac caaatgtggg gatcaatcga acaatacaac acagtcgtat
     3481 ggcattggcc tatcgcggtc tatctctttt tggcgggtct ctcggctgga gccatcatct
     3541 cagcgattat catcaaatgg atgaaaggca atgagagctc tccttgggat ggaatcatca
     3601 aagcgggcgc gctgattgcg cctttgacga tcggagcagg acttttgctc ttgatttttg
     3661 acctaacgcg accgcttcat ttctggaagc tgttgatttt ctacaatttc agttctgtaa
     3721 tgaccttggg cgttttggcg ctttttgcct atttccccgt ggtgcttatc ttcttgttgg
     3781 gtgtattcaa aaaagagctt tgcgatgagg gaccctttgg cttccttgca cccctagcca
     3841 atatcgccta tagcatggct agacctcttg agattgtcac ttttgttttg gccattggcg
     3901 taggagctta cacaggattc ttgctctcag cgatgtatag ctatccgttg ctcaatacgc
     3961 ctatcttacc tttgcttttc ctcgcttcag gaatctcagc gggaatctca ggcaaccttc
     4021 tcatcggtct tctcttcttt ggtaagagca ccaaaggtga gaatgtaggc tatcttcatg
     4081 ggcttgactt caaggttatc ttgtttgagg cctttttgct ctttattctt tttgtgggaa
     4141 tgtattacca aggtggaagc accgctgaag tcgccaaagc ggcgctgacc acaggagggc
     4201 tggcttcact cttctggctt ggtgtggctg ggatgggctt ggcgttgcct gtagtgctca
     4261 atgtcgcgct tcctcatgga atcaagcact ccagcggctt tgtcatgctc aacgcgctca
     4321 ttgtgctagc gggtgtgatg gcacttcgct tctatatcct ctacgcggga cagactttcg
     4381 taggctgatc cctctacttt ttgatgaccc ttggaggaaa atcctctggg ggttcccctt
     4441 tataatctca atctatggct gttggagctg aggataaatc gacaatgagg cgataaccta
     4501 ttcctttgtg cgtttggata gagctagcag gaatctttag acgcaattgt cgaatcatgg
     4561 agcgaatatt ctccctaggc acctctccct cttcccaaac atactcttca atctcctcaa
     4621 aactcacaag taggcctctt gccctcacca aaagctcaag cagacgcttc tctttggggc
     4681 taaggagaag gctttggcct tcacgcaaaa tcaaagcgtt ttctagatct aaaaatgtct
     4741 cttcacccaa aggaatgaga gggtgaagtt tgccgcagag gcggtgggtt ttaaaaagaa
     4801 gctcatccaa aaagaagggt tttttgagaa agtccgcaca tccatggcga taggcttggc
     4861 ggatcacatc tagatcgaca aaagagctga tgatgatcac aggggccttg atgaagcgtc
     4921 cttgaatcag cgcgagcaac tctaga
//