From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Vibrio parahaemolyticus flagellar protein (lafL and lafS) genes;
Date: 29 Dec 92 20:59:11 GMT
Distribution: bionet
Checksum: 07703 7

LOCUS       VIBLAFLSTU   2438 bp ds-DNA   Circular  BCT       11-DEC-1992
DEFINITION  Vibrio parahaemolyticus flagellar protein (lafL and lafS) genes;
            flagellin (lafT and lafU) genes.
ACCESSION   L06177
KEYWORDS    RNA polymerase; flagellar protein; flagellin; homologue; lafL gene;
            lafS gene; lafT gene; lafU gene; sigma subunit.
SOURCE      Vibrio parahaemolyticus (strain BB22) (library: pLAFRII) DNA.
  ORGANISM  Vibrio parahaemolyticus
            Prokaryotae; Facultatively anaerobic gram-negative rods;
            Vibrionaceae.
REFERENCE   1  (bases 1 to 2438)
  AUTHORS   McCarter,L.L. and Wright,M.E.
  TITLE     Identification of genes encoding components of the swarmer cell
            flagellar motor, propeller, and a sigma factor controlling
            differentiation of Vibrio parahaemolyticus
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     RBS             229..232
                     /gene="lafS"
     RBS             967..971
                     /gene="lafT"
     RBS             1826..1831
                     /gene="lafU gene"
     CDS             <1..227
                     /gene="lafL"
                     /note="carboxy terminus of a protein homologous to the
                     FliL flagellar protein of Salmonella typhimurium"
                     /product="flagellar protein"
                     /codon_start=3
                     /translation="AGRPERIKDIDNYMPMVQNSLLKLFSDKTFDELQQTGAIDILQN
                     EVKQTLLVAFAKTDIVRDIDDVLLTKYVVQ"
     CDS             240..968
                     /gene="lafS"
                     /note="lateral flagellar sigma subunit of RNA polymerase"
                     /product="flagellar protein"
                     /codon_start=1
                     /translation="MLDMNPQETYTAPEEVNTPSRPIDENALLQRHQVMVKRVVNQLR
                     VHATSHCSIEDMQQIGLIALVEAGRRYGDIDDTHFPAFAVCRVRGAILDELRRLDWRS
                     RKTRQQAHELNDVTRDLTRSLGRMPTDSEIIKALGTDEQDYYNRQNAALAGEMQSLDQ
                     LMENSTDSHFGGQYDGMEHEHIRRSLDSALGRLSKRDQLLLTLFYQHELNLHEIALVL
                     DLTPPRICQLHKQALKQLNQLMSS"
     CDS             979..1836
                     /gene="lafT"
                     /note="lateral flagellar protein homologous to flagellar
                     MotA protein of Escherichia coli"
                     /product="flagellin"
                     /codon_start=1
                     /translation="MQKFLGVLTILVCVFGGYMWAGGKLGAIWQPAEFLIIIGAAAGS
                     LIIGNPPHVLKEMRQQVPATIKGPTEEYEYYMELMALLNNLLETARSRGFKFLDSHIE
                     APEQSSIFLMYPLVSEDHRLISFITDNLRLMAMGQMSPHELEGLLEQEIEAIQNELLL
                     PSRSLQRTAEALPGFGILAAVGGIIITMQAIDGSIALIGYHVAAALVGTFIGIFGCYC
                     GLDPLSNAMAQRVKRNMTAFECVRATLVAYVAKKPTLLAIDAGRKHIQLDIKPTFNQM
                     EKWLAEQEG"
     CDS             1836..>2438
                     /gene="lafU"
                     /product="flagellin"
                     /codon_start=1
                     /translation="MQKQEHVVFKRAKAHGHDEPHGGAWKVAFADFMIALMALFLVLW
                     VMQVVDKEERKAIVAHLHSSSVFDKSYGNPFDTSQSISPIDLAQDSSVPSKHNSNHVV
                     SSYFQGDGDGPEINSLVPGTFDTQEQLAALAKVIEEMTAQINAQGNVNVTVTPQGLRI
                     VLQDDYKQHMFSRGGAELTPFFEDLLLALAPLFEQVTNPLI"
BASE COUNT      675 a    602 c    567 g    594 t
ORIGIN      
        1 ctgcaggtcg accagaacgt attaaagaca tcgataacta catgccaatg gttcaaaact
       61 cgcttttgaa gctgtttagc gacaaaactt tcgatgaact acaacagaca ggcgcgatcg
      121 atattctgca aaacgaagtg aagcaaacgc tgttggtagc ctttgcgaag actgacatcg
      181 tgcgtgatat cgatgatgta ctgctgacga aatacgttgt gcaataacgg agcacaacca
      241 tgttagacat gaaccctcaa gaaacgtaca ccgcaccaga ggaagtgaac acaccatcac
      301 gcccgattga tgaaaacgca cttttgcaac gtcatcaagt gatggtgaaa cgcgtagtta
      361 accagcttcg cgttcatgcc acttcccatt gcagtatcga agatatgcag caaattggtc
      421 ttatcgcgct ggttgaagca ggacgtcgtt atggagacat tgacgatact cacttcccgg
      481 cgtttgcggt ttgccgtgta cgcggcgcga tcttagatga actgcgccgt ttagactggc
      541 gctcacgtaa gacacgtcag caagcccatg agcttaacga cgtcacacgt gacctcaccc
      601 gctcgttagg tcgcatgcca acggactcag aaatcatcaa agctctcggc accgacgagc
      661 aagattatta caaccgtcaa aacgcggcgc ttgcaggtga aatgcaaagt ctcgatcaac
      721 tgatggaaaa cagcaccgac agccactttg gtggtcaata cgacggcatg gagcacgagc
      781 acattcgtcg tagcttagac agcgcactag gccgattgtc aaagcgagat caattgctac
      841 tgacgctgtt ttatcaacac gaactcaatt tacacgaaat agccctcgtc ttggatctga
      901 cgccacctcg aatttgtcag cttcataaac aagcgctgaa acaactcaac caattaatgt
      961 cctcttagga gtcacaagat gcaaaagttt ttaggagtcc tcactatcct tgtgtgtgtc
     1021 ttcggcggat acatgtgggc tggcggtaag ctaggtgcaa tttggcaacc agcagaattt
     1081 ctgatcatca tcggcgcggc agcgggttct ctcattattg gtaacccacc acacgtgcta
     1141 aaagagatgc gccaacaagt gccagcaacc atcaaaggtc caactgaaga gtacgagtac
     1201 tacatggagc tgatggcgct attaaacaac ctgctagaaa cagcgcgtag ccgtggtttc
     1261 aagtttttgg attctcacat cgaagcacca gaacaaagct ctatcttcct gatgtacccg
     1321 ctagtttctg aggatcatcg tctgatttcg ttcattactg ataatttacg tttaatggcg
     1381 atgggacaaa tgtcgcctca tgaattagag ggactgttag aacaagaaat cgaagctatc
     1441 cagaacgaac tgctgctgcc ttcacgctca ttacagcgta ccgcagaagc actacctggt
     1501 ttcggtattc ttgccgcagt tggtggcatc atcatcacta tgcaagccat agatggttct
     1561 attgcgctca ttggctatca cgtagccgct gcccttgtag gtacattcat tggtattttt
     1621 ggttgttact gtggtctaga tccactaagt aacgccatgg ctcagcgagt gaaacgtaat
     1681 atgaccgctt ttgaatgcgt acgcgcaacg ctcgttgctt acgtcgctaa aaagccaaca
     1741 ttgctcgcca tcgacgctgg ccgtaaacac attcagctag acatcaaacc aacattcaac
     1801 caaatggaga agtggttagc tgaacaggag ggataatgca aaaacaagag catgtagtgt
     1861 tcaaacgtgc caaagctcac ggccatgacg aacctcatgg cggcgcgtgg aaagtcgcgt
     1921 ttgctgactt catgatcgcc ctaatggcgc tgtttcttgt gctttgggtc atgcaagtgg
     1981 tggataaaga agagcgtaaa gcgattgttg cccacttgca tagctcaagc gtgttcgaca
     2041 agagttacgg caacccattt gatacctcac aaagtatctc tcctatcgac ttggcacagg
     2101 actcttctgt gccaagtaaa cacaattcca atcacgttgt gagctcttat tttcaaggtg
     2161 acggtgatgg tccagaaatt aactctctag ttcctggcac cttcgacacc caagagcaac
     2221 ttgctgctct ggcaaaagtt atcgaagaga tgaccgcgca gatcaacgcg caaggaaacg
     2281 tcaacgtgac tgtgacacca caaggcttgc gtatcgtgtt gcaagacgat tacaaacagc
     2341 acatgttcag tcgtggtgga gccgagctta cgccattttt tgaagattta ctgttggcac
     2401 ttgcccctct tttcgagcaa gtaaccaacc cattgatc
//