From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: virK=virulence-associated gene [Shigella flexneri, 2a YSH6000T,
Date: 27 Jan 93 05:51:54 GMT
Distribution: bionet
Checksum: 07915 4

LOCUS       S114617      1642 bp    DNA             BCT       26-JAN-1993
DEFINITION  virK=virulence-associated gene [Shigella flexneri, 2a YSH6000T,
            pMYSH6000, Plasmid, 1642 nt]
ACCESSION   S45618
KEYWORDS    .
SOURCE      Shigella flexneri pMYSH6000 2a YSH6000T
  ORGANISM  Shigella flexneri
            Unclassified.
REFERENCE   1  (bases 1 to 1642)
  AUTHORS   Nakata,N., Sasakawa,C., Okada,N., Tobe,T., Fukuda,I., Suzuki,T.,
            Komatsu,K. and Yoshikawa,M.
  TITLE     Identification and characterization of virK, a virulence-associated
            large plasmid gene essential for intercellular spreading of
            Shigella flexneri.
  JOURNAL   Mol. Microbiol. 6, 2387-2395 (1992)
  STANDARD  full automatic
COMMENT     This entry [NCBI gibbsq 114617] was created by the journal scanning
            component of NCBI/GenBank at the National Library of Medicine.
            This sequence comes from Fig. 7.
FEATURES             Location/Qualifiers
     CDS             373..1323
                     /note="Description: virulence-associated gene;  For the
                     protein sequence (NCBI gibbsq 114621):  Method: conceptual
                     translation supplied by author.  This sequence comes from
                     Fig. 7."
                     /gene="virK"
                     /product="VirK"
                     /codon_start=1
                     /translation="MFSVSNLSFIGFLKRIVFSSDSLPGKWEHRKFRFMYILRCAINP
                     VASIRYYYELRSLQCIEDILAIQPTLPARIHRPYLHKGGRAWSRGQYILEHYRFVQNL
                     PEKYSEFLFPQKSVSLVQFIGKDGEDFDIQCSPSGFDREGELMLSLFFNKIVIARLTF
                     SVILTQNGHTAFIGGLQGAPKNTGPDIIRCATRACYGLFPKRIIFEAFCALMKACNVS
                     ECLAVSEHSHVFRQLRYWYQKRKTFVAVYSDFWESVAGKTCGDWYKLPTQVVRKPLSN
                     IASKKRSEYRKRYALLDYIHETAIRSLDAYPVNSEHQDLN"
BASE COUNT      451 a    311 c    376 g    504 t
ORIGIN      
        1 gcatgcatga tgatgttttt attgctgaca atgtttttcc tgccgccccc gtatatcggg
       61 ttgccagtct ggtggttctg ccttcagaaa acgaatcgtt tggtatggta ctggcagaag
      121 catcggcatt ttctgtgcct gtactggcca gtcagattgg tggaatccct gatgttattc
      181 agaataacca gaccgggaca ttgttaccag caggtaataa gcacgcatgg atgtgcgccc
      241 tgaatgattt ttttaatgac cctgggcgtt tttatcagat ggctcgccag gcaaaacagg
      301 atatagaaga gcggtttgat attaataaaa ctgcgttaaa aatactcaca ttagcgaagc
      361 acaagtaaca atatgttttc tgtaagtaac ttatcattta tcggtttcct taaaaggatt
      421 gttttttcct cagattcact cccggggaag tgggaacaca gaaaattccg gttcatgtac
      481 attttgcgat gtgctataaa tccggttgcc agtattcgat attattacga actgcgttcc
      541 ttgcagtgca ttgaggatat tctggcaata caacccacgt tgccagcaag aattcatcga
      601 ccttatttgc ataaaggggg gcgagcctgg tcccgggggc aatatattct tgagcattat
      661 cgctttgtgc aaaatttgcc agaaaaatat tctgaatttt tgtttccaca aaaatcggta
      721 tcattagtcc aattcatcgg gaaagatgga gaggattttg atatccagtg ttctcccagt
      781 ggttttgacc gggaaggaga gttgatgttg tcattgtttt tcaataaaat agtgattgcc
      841 cggttaacat tttcagtcat tctgactcaa aatggacaca ccgcttttat tggcggatta
      901 cagggagccc ccaaaaatac cggacctgat attattcggt gcgcaacccg ggcctgttat
      961 gggcttttcc caaaacggat tatttttgaa gcattttgtg cgttgatgaa agcttgtaat
     1021 gtctctgagt gtctggcagt cagtgagcac agtcatgttt tccggcaatt gagatactgg
     1081 tatcagaaac gcaaaacctt tgttgctgtc tacagtgatt tctgggagtc tgtagcaggg
     1141 aaaacctgtg gtgactggta caaattacca acacaggtgg ttcgaaagcc gctaagcaat
     1201 atagctagta aaaaacgctc tgagtatcga aaacgatacg ctttgctgga ttatatccat
     1261 gaaactgcta tccgctcttt ggatgcatat cctgtaaact cagaacatca ggacttaaat
     1321 taaggtttga tgtattctct gaataaaata ttaatgatga ttttggtagg ggcattcgca
     1381 ctaaataatg aaaaaataca aatccgagtt tattcctgaa tttaagaaaa attatctttc
     1441 ccctgtttac tggtttacat ggttcgtttt gggaatgatt gcaggtattt caatgtttcc
     1501 cccttcattc agagatcctg tcttggccaa aatagggcgt tgggtgggca gattgagcag
     1561 aaaagctcgt cgcagggcga cgattaattt atcgctttgt ttcccggaaa agagtgatac
     1621 agaacgggaa ataattgtcg ac
//