From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Trypanosoma brucei NADH:ubiquinone oxidoreductase complex I (ndhK)
Date: 27 Aug 92 10:01:02 GMT
Distribution: bionet
Checksum: 03600     4

LOCUS       TRBNDHK       961 bp ds-DNA             INV       27-AUG-1992
DEFINITION  Trypanosoma brucei NADH:ubiquinone oxidoreductase complex I (ndhK)
            gene, complete cds.
ACCESSION   L00585
KEYWORDS    NADH:ubiquinone oxidoreductase complex I.
SOURCE      Trypanosoma brucei (strain EATRO 164) procyclic DNA.
  ORGANISM  Trypanosoma brucei
            Eukaryota; Animalia; Protozoa; Sarcomastigophora; Mastigophora;
            Kinetoplastida; Trypanosomatina; Trypanosomatidae.
REFERENCE   1  (bases 1 to 961)
  AUTHORS   Peterson,G.C., Souza,A.E. and Parsons,M.
  TITLE     Characterization of a Trypanosoma brucei nuclear gene encoding a
            potential subunit of NADH: Ubiquinone oxidoreductase (complex I)
  JOURNAL   Unpublished (1992)
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     misc_RNA        <1..1
                     /function="site of spliced leader addition"
                     /evidence=EXPERIMENTAL
                     /citation=[1]
     misc_RNA        31
                     /function="site of spliced leader addition"
                     /evidence=EXPERIMENTAL
                     /citation=[1]
     misc_RNA        48
                     /note="one of three PCR cDNA sequenced had two A residues
                     inserted at position 48"
                     /note="putative"
                     /citation=[1]
     CDS             143..751
                     /product="NADH:ubiquinone oxidoreductase"
                     /gene="ndhK"
                     /note="putative"
                     /codon_start=1
                     /translation="MLRRTSFNFTGRAMISRGSPEWSHRLDLKKGKKTTMMHKLGTSK
                     PNNALQYAQMTLHDLTEWCLAYSPWPLTFGLACCAVEMMHAYASRYDLDRFGIVPRPT
                     PRQAEIIIVSGTVTNKMAPILRNIYVQMVNPKWVISMGSCANGGGYYHFSYAVLRGCE
                     RAIPVDFWIPGCPPSAESLVFCLHTLQKKIRWHEIQKYSVRD"
                     /citation=[1]
BASE COUNT      260 a    197 c    220 g    284 t
ORIGIN
        1 ataataaatt tacgtacact tacaaaatag gggatacaaa aaaaaaaact tcctctactg
       61 cggggttaga tagaggacaa agtaggaaat agaatctttt ttaaaaaaaa aaagaaagaa
      121 gaggatagat aaatagtttg caatgcttcg tcgcacgtcg tttaatttca cagggcgtgc
      181 gatgatttcg cgcggcagcc ccgagtggtc gcatcgtttg gatctcaaga aggggaaaaa
      241 gacgacaatg atgcacaaac ttggcaccag taaacccaat aatgcattgc agtatgcaca
      301 aatgacttta cacgacctta cggagtggtg ccttgcttac tcgccgtggc ctctcacctt
      361 cggtttggca tgttgcgccg tagaaatgat gcatgcatat gcttctcggt atgatttgga
      421 tcgttttggt attgtaccgc gtccaacacc ccgtcaagca gaaattatta ttgtctccgg
      481 cactgtgaca aataaaatgg ctccaatctt gcggaacata tatgtgcaaa tggtgaaccc
      541 caagtgggtt atttccatgg gaagctgcgc taacggtggt ggttactatc acttctccta
      601 cgccgtgctc cgaggctgtg aaagagcaat accagttgat ttctggattc ccggttgccc
      661 tccttcagcg gaaagtcttg tcttttgctt gcatacatta cagaagaaaa tacgctggca
      721 cgaaattcaa aagtattctg ttcgcgatta aagaatcatg gtacaatagg agtttctatt
      781 tttggtgtgc atatgtgttt gtgtgtgtgt tacccgatag aaaagattta catcgttacg
      841 gccgttggca aactagcggc aggagtctgc gggaaatcaa atccctcttt ttctccgtcc
      901 ttttttgact tttccctttc cttcttttgt tttggatcag gggatggtgt tccattcttt
      961 t
//