From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: M.sativa leghemoglobin gene for leghemoglobin (clone BRC56)
Date: 25 Jul 92 10:02:12 GMT
Distribution: bionet
Checksum: 33552     6

LOCUS       ALFLEGBRC    2358 bp ds-DNA             PLN       25-JUL-1992
DEFINITION  M.sativa leghemoglobin gene for leghemoglobin (clone BRC56)
ACCESSION   X54089
KEYWORDS    leghemoglobin; oxygen binding protein; transport protein.
SOURCE      Medicago sativa DNA.
  ORGANISM  Medicago sativa
            Eukaryota; Plantae; Embryobionta; Magnoliophyta; Magnoliopsida;
            Rosidae; Fabales; Fabaceae.
REFERENCE   1  (bases 1 to 2358)
  AUTHORS   Davidowitz,E.J., Creissen,G., Lang-Unnasch,N., Vincze,l. and
            Kiss,G.B.
  TITLE     Heterogeneity of the Alfalfa, Medicago sativa, leghemoglobin gene
            family
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
COMMENT     *source: tissue=germinating seeds; *source: clone_library=alfalfa
            genomic library; *source: clone=BRC56;
            From EMBL    entry MSLEGBRC;  dated 04-MAY-1992.
FEATURES             Location/Qualifiers
     CDS             join(<181..320,462..573,1406..1513,1995..2123)
                     /product="leghemoglobin"
                     /gene="leghemoglobin"
                     /codon_start=1
                     /translation="LQIQIAKQKQKNKKRNMGFTEKQEALVNSSFESFKQNPGYSVLF
                     YTIILEKAPAAKGMFSFLKDSAGVQDSPKLQAHAGKVFGMVRDSAAQLRATGGVVLGD
                     ATLGAIHIQNGVVDPHFVVVKEALLKTIKESSGDKWSEELSTAWEVAYDALATAIKKA
                     MS"
                     /partial
     mRNA            join(<181..320,462..573,1406..1513,1995..>2358)
                     /gene="leghemoglobin"
                     /partial
     TATA_signal     143..150
     exon            181..320
                     /number=1
     prim_transcript 182..>2358
                     /gene="leghemoglobin"
     intron          321..461
                     /number=1
     exon            462..573
                     /number=2
     intron          574..1405
                     /number=2
     exon            1406..1513
                     /number=3
     intron          1514..1994
                     /number=3
     exon            1995..>2358
                     /number=4
BASE COUNT      850 a    322 c    323 g    863 t
ORIGIN
        1 tttaaagaaa actcgtttga ttaagttttt gaaaagatta ttgtctcttt aatgttgtca
       61 atagccattt ccacaagcca atagattcat taaaaaaata aatcacaaaa aatactacac
      121 ctcttttcac ctctaatttt tctatataaa catgtatgga atgtacaata tattattaca
      181 ttgcaaatac aaatagcaaa acaaaaacaa aaaaataaga agagaaatat gggtttcacc
      241 gagaaacaag aggctttagt gaatagctca tttgaatcat ttaaacaaaa tcctggttat
      301 agtgttttgt tctacactat gtaagtttga tatcttttga gtgtttatgt ttgtattttc
      361 tctttttgtt aattattggt ttaaaagaga agacataaat taacaataat attgtataaa
      421 aaaattaaca ataatatttt tggttgcttt atttaaaata gtatattgga gaaagcacct
      481 gcagcaaagg gtatgttctc ttttcttaag gactcagctg gagtacaaga tagtcctaaa
      541 cttcaagccc atgcaggaaa agtttttgga atggtgagtg taattaaaca tcgattaaat
      601 tttttccttc ttttatttat tcttcattca acgacgattt tactcttaaa ataataacta
      661 tgaaatatta ttttaaataa ctatacttcc tatttgtgtt ttattgtaac tgcatatcct
      721 tattattttg tgcttcgtat tccactttta taaagttttg aatccccgtg tttcagtagt
      781 ctaaattaga tattccacaa ttcttaattg ttatatcaaa acctctatta ctgttcaata
      841 tttaactcca agatcctaca tatattcaat agaagttggc actaactcat accagattca
      901 aaagctaata tagtcaatga agacccttca agcatgatac ttgcttgccg tataaacctt
      961 ttcatcacgt caccatgata cttccttggc cgtgttggct tatcatgcta taacttaagt
     1021 caacataaat ttatactcat tatggctttt agcattaaaa attactccaa tatgattaat
     1081 ggatggagaa aaagatgata ttgacaattg tgatatctag gagacatcat ccacacaatt
     1141 gaaatacatg aaatcaaaat ttcacaacgg cgggagattg aaaacgcaat aaaacatcta
     1201 tttaatcaag tctaatttta ttttttacta gttggaaaga ttgttcgatt aaatatagaa
     1261 aatgaattaa tatttttcta cttaagaata tataattaca ttatattaat aggaataata
     1321 acaaattcta tttgtacttt ttttaatagt tttcactaac acgaaaatta ttgatcttaa
     1381 gatatttata ccttttgaat tataggtacg cgattcagct gctcaactcc gagccacagg
     1441 gggagtagta ttgggagatg ctaccttggg tgctatccac attcagaatg gagttgttga
     1501 tcctcatttt gtggtatgat aaaatcatac aatatttcaa tagattcatt cttagttttt
     1561 tttactgcct aatggcattt attctttaat tcaatattac aacaataaat aaaatccacc
     1621 atttcatgcc actttttcat attataatat aaatattgaa aagaaattat taaagaaatt
     1681 tatgtaaact atgtttataa agataaaatt aacaatctta tgataaaata agatttggct
     1741 tgtctaaatt gggtagtaag taaagtgaca aataaatggt tgatattata acaaatcata
     1801 agcggttctt caataaaata atgtgttgat attttaacca ctcatgattt ggtattcagt
     1861 tctttaaaaa ttattgagtt gttgttattc aataaaataa ataatttttc aaaaaatatt
     1921 ttccattttt gttaacatgc ttttttcaag tggtaaattg ttaatccaaa attaatgctt
     1981 ttggcacctt acaggtggtt aaagaagctt tgttgaaaac aataaaggaa tcatcaggag
     2041 ataaatggag cgaagaattg agcactgctt gggaagtagc ttatgatgca ttggcaactg
     2101 caattaagaa ggcaatgagt taaacttggg atgatatata gttacaactc aatttataat
     2161 aaataaaagt tgtattacta aaacttgttt aacaagtttc tataataaat attattgaaa
     2221 tgactattaa atagttatta tatttatgct agtgcatttc ttggtaaaaa aatcccatca
     2281 atattcaaac aactattaaa tagttattat atttgtgcta tgtgcctttt ctttaatcca
     2341 ccaccaagag tgagtctt
//