From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Mus musculus alpha-2-macroglobulin mRNA, complete cds.
Date: 26 Jan 93 05:48:43 GMT
Distribution: bionet
Checksum: 52849 10

LOCUS       MUSMCGLBA    4674 bp ss-mRNA            ROD       25-JAN-1993
DEFINITION  Mus musculus alpha-2-macroglobulin mRNA, complete cds.
ACCESSION   M93264
KEYWORDS    alpha-2-macroglobulin.
SOURCE      Mus musculus cDNA to mRNA.
  ORGANISM  Mus musculus
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Rodentia; Myomorpha; Muridae; Murinae.
REFERENCE   1  (bases 1 to 4674)
  AUTHORS   van Leuven,F., Torrekens,S., Overbergh,L., Lorent,K., de
            Strooper,B. and van den Berghe,H.
  TITLE     The primary sequence and the subunit structure of mouse
            alpha-2-macroglobulin deduced from protein sequencing of the
            isolated subunits and from molecular cloning of the cDNA
  JOURNAL   Eur. J. Biochem. 210, 319-327 (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     sig_peptide     50..121
                     /codon_start=1
     mat_peptide     122..4534
                     /product="alpha-2-macroglobulin"
                     /codon_start=1
     polyA_signal    4650..4655
     CDS             50..4537
                     /product="alpha-2-macroglobulin"
                     /codon_start=1
                     /translation="MRRNQLPTPAFLLLFLLLPRDATTATAKPQYVVLVPSEVYQESL
                     KRPCVSLNHVNETVMLSLTLEYAMQQTKLLTDQAVDKDSFYCSPFTISGSPLPYTFIT
                     VEIKGPTQRFIKKKSIQIIKAESPVFVQTDKPIYKPGQIVKFRVVSVDISFRPLNETF
                     PVVYIETPKRNRIFQWQNIHLAGGLHQLSFPLSVEPALGIYKVVVQKDSGKKIEHSFE
                     VKEYVLPKFEVIIKMQKTMAFLEEELPITACGVYTYGKPVPGLVTLRVCRKYSRYRST
                     CHNQNSMSICAEFSQQADDKGCFSQVVKTKVFQLSQKGHDMKIEVEAKIKEEGTGIEL
                     TGIGSCEIANALSKLKFTKVNTNYRPGLPFSGQVLLVDEKGKPIPNKNITSVVSPLGY
                     LSIFTTDEHGLANISIDTSNFTAPFLRVVVTYKQNHVCYDNWWLDEFHTQADHSATLV
                     FSPSQSYIQLELVFGTLACGQTQEIRIHYLLNEDIMKNEKTLTFYYLIKARGSIGNLG
                     SHVLSLEQGNMKGVFSLPIQVEPGMAPEAQLLIYAILPNEELVADAQNFEIEKCFANK
                     VNLSFPSAQSLPASDTHLKVKAAPLSLCALTAVDQSVLLLKPEAKLSPQSIYNLLPGK
                     TVQGAFFGVPVYKDHENCISGEDITHNGIVYTPKHSLGDNDAHSIFQSVGINIFTNSK
                     IHKPRFCQEFQHYPAMGGVAPQALAVAASGPGSSFRAMGVPMMGLDYSDEINQVVEVR
                     ETVRKYFPETWIWDLVPLDVSGDGELAVKVPDTITEWKASAFCLSGTTGLGSSSTISL
                     QAFQPFFLELTLPYSVVRGEAFTLKATVLNYMSHCIQIRVDLEISPDFLAVPVGGHEN
                     SHCICGNERKTVSWAVTPKSLGEVNFTRTAEALESQELCGNKLTEVPALVHKDTVVKS
                     VIVEPEGIEKEQTYNTLLCPQDTELQDNSSLELPPNVVEGSARATHSVLGDILGSAMQ
                     NLQNLLQMPYGCGEQNMVLFVPNIYVLNYLNETQQLTEAIKSKAINYLISGYQRQLNY
                     QHSDGSYSTFGNHGGGNTPGNTWLTAFVLKAFAQAQSHIFIEKTHITNAFNWLSMKQK
                     ENGCFQQSGYLLNNAMKGGVDDEVTLSAYITIALLEMPLPVTHSAVRNALFCLETAWA
                     SISQSQESHVYTKALLAYAFALAGNKAKRSELLESLNKDAVKEEDSLHWQRPGDVQKV
                     KALSFYQPRAPSAEVEMTAYVLLAYLTSESSRPTRDLSSSDLSTASKIVKWISKQQNS
                     DGGLLLTQDTVVALQALSKYGSATFTRSQKEVLVTSRSSGTFSKTFHVNSGNRLLLQE
                     VRLPDLPGNYVTKGSGSGCVYLQTSLKYNILPVADGKAPFALQVNTLPLNFDKAEDHR
                     TFQIRINVSYTGERPSSNMVIVDVKMVSGFIPMKPSVKRLQDQPNIQRTEVNTNHVLI
                     YIEKLTNQTLGFSFAVEQDIPVKNLKPAPIKVYDYYETDEFTVEEYSAPFSDGSEQGN
                     A"
BASE COUNT     1332 a   1156 c   1059 g   1127 t
ORIGIN      
        1 cagagttcgg gggctgaggg ctcagacgtt cttctctgcc ctctccacca tgaggagaaa
       61 ccagctgccc acaccagctt ttcttttact gttcctgctt cttcccagag atgccaccac
      121 agctactgca aaaccacaat atgtggtgct ggtcccgtca gaggtctatc aggagtccct
      181 gaaaaggcct tgtgtcagcc tcaaccatgt gaatgagact gtgatgctca gcttaactct
      241 agagtatgca atgcagcaaa cgaagctcct cacagaccag gctgtggata aggactcctt
      301 ctactgcagc cccttcacga tctcaggttc acctttaccc tacacattca ttactgtgga
      361 gataaaagga ccaacgcagc gcttcataaa gaagaagtca atacaaataa taaaagctga
      421 gagcccagtc tttgtccaga cagacaaacc catatacaaa ccaggacaga tagtgaaatt
      481 ccgagttgtt tctgtggaca tcagttttcg cccgttgaat gaaacgttcc ctgtcgttta
      541 tattgagact cccaagagga accgaatttt tcaatggcaa aatatccatc tggcaggagg
      601 actccaccag ctctctttcc cactgtctgt tgagccagct ctgggtatct acaaggttgt
      661 agtgcagaag gactcaggga agaaaataga acactccttt gaggtgaagg aatacgtttt
      721 acccaaattt gaggtgataa taaaaatgca gaagactatg gctttcctgg aagaggaact
      781 tcctataact gcttgtggcg tatacacata tggaaagcct gttcccggtc tggtgacatt
      841 gagagtgtgc agaaaatatt cacgataccg ctccacctgc cataaccaaa actcaatgag
      901 tatctgtgcc gaattcagcc aacaggcaga tgataaagga tgtttcagcc aagttgtaaa
      961 aaccaaagta ttccagctca gccaaaaagg ccacgacatg aagatagagg tggaagccaa
     1021 aatcaaagag gaaggcacag gcatagaact aactggtatt ggatcatgtg aaatagcaaa
     1081 tgccttaagc aaactgaaat ttactaaagt aaatacaaat tacaggcctg ggctaccttt
     1141 ctctggtcag gttcttcttg ttgatgagaa aggtaaacca atccccaaca aaaacataac
     1201 ttccgtcgtg tctccacttg ggtacctgtc catttttact acggatgagc atggcttggc
     1261 taacatttcc attgacactt ccaacttcac agctccgttt ttgagagttg tggtcaccta
     1321 caagcagaac catgtctgct atgataactg gtggcttgat gaatttcaca cacaggcaga
     1381 ccattctgca actctagtct tttctccaag ccagagttat atccaacttg aacttgtttt
     1441 tggtactttg gcctgtgggc aaactcagga gattcggata cactacctct tgaatgaaga
     1501 tatcatgaag aatgaaaaga ctttaacctt ttactatctg atcaaagcaa ggggaagcat
     1561 cggcaactta ggaagccatg tgttgtcgct tgaacaagga aacatgaaag gagtcttttc
     1621 cctcccaatt caagtggagc caggcatggc tcccgaggct cagctgctca tttatgctat
     1681 tttacctaat gaagaacttg tcgctgatgc tcagaatttt gaaatcgaga agtgttttgc
     1741 caacaaggta aatttgagtt tcccatcagc acagagcctg ccagcctctg acacccacct
     1801 gaaggtcaaa gccgcgcctc tgtccctctg tgccctcact gcagtagacc agagtgtgct
     1861 gctactgaag cccgaagcca agctctcacc tcagtcaatc tacaatttgc tgccaggaaa
     1921 gactgtccag ggtgccttct ttggtgtccc agtgtacaaa gaccatgaga actgcatcag
     1981 cggagaagac atcactcaca atgggatcgt gtacacgcca aagcactctc tgggcgataa
     2041 tgatgcacac agcatttttc agtctgtagg aataaatatt tttaccaact ccaaaatcca
     2101 caaaccacgc ttttgtcaag agtttcaaca ctatccggca atgggaggag tggcacctca
     2161 agccttagct gtggctgctt caggcccagg atccagcttc agagcaatgg gcgtgccgat
     2221 gatggggttg gactactctg atgaaattaa tcaggtggtg gaagtaagag agacagtgcg
     2281 gaagtacttc cctgagacct ggatctggga cctggtgcca ctggacgtat ccggggacgg
     2341 tgaattggcg gtaaaggtcc ccgacaccat cactgagtgg aaggccagtg cattctgctt
     2401 gtctggaacc actggccttg gctcttcctc caccatctcc cttcaagcct tccagccctt
     2461 cttcttggag ctcactctcc catactctgt ggtgcgaggg gaagccttta ccctcaaagc
     2521 caccgtgctc aattacatgt ctcactgcat tcagatccga gtggacctag agatttctcc
     2581 tgatttcctg gcagtcccag tggggggcca tgaaaactct cattgcatct gtggaaatga
     2641 aaggaaaacc gtgtcctggg ctgtgacccc aaagtcgctg ggggaggtga acttcacacg
     2701 taccgcagaa gccttggagt ctcaagaatt gtgtggcaat aagttgacag aagtgccagc
     2761 ccttgtacac aaggacactg tggtgaagtc cgtaatagtt gagcctgaag gaattgagaa
     2821 ggagcaaacg tacaacacac tgttatgccc acaagatact gagttacaag ataattcctc
     2881 actggagctt ccacccaatg tggttgaagg atctgccagg gctacacatt ccgttttggg
     2941 tgatatactg ggctctgcaa tgcaaaacct ccagaatctt ctccagatgc cctatggctg
     3001 tggggaacaa aacatggtcc tttttgtccc taacatctat gttctgaact atctgaacga
     3061 gacacagcag ctgacagagg cgatcaagtc caaagccatt aactacctca tcagtgggta
     3121 ccagaggcag ctgaactatc agcacagtga tggctcgtac agcacattcg ggaaccatgg
     3181 tggtggcaac actccgggaa atacttggct cactgcgttt gtgctcaagg cctttgctca
     3241 agctcagtcg cacatcttta tagagaagac acacatcaca aatgctttca actggctctc
     3301 catgaaacaa aaggagaatg gctgtttcca acagtctgga tatctgctta acaatgcgat
     3361 gaagggtggt gtggatgatg aagtgactct ctctgcctac atcaccattg ctctgctgga
     3421 gatgcctctg cctgtcacgc acagtgctgt gcgaaatgct ctattttgcc tggaaacggc
     3481 ctgggcctcc atctcacaga gccaggaaag tcatgtctat accaaggcat tgctggccta
     3541 tgcctttgcc ctggcaggaa acaaggcgaa gagaagcgaa ctgcttgaat ccctaaacaa
     3601 agatgctgtg aaggaagagg attcactgca ctggcaacgc cctggggatg ttcagaaagt
     3661 gaaggcctta tctttctatc aacctcgggc cccttctgcc gaagtggaga tgacagctta
     3721 cgtgcttctc gcctatctaa cctctgagtc ttcccggccc acacgggacc tgtcttcatc
     3781 agacctgtca acagcatcca agattgtgaa gtggatcagc aagcaacaaa actcggacgg
     3841 ggggcttctc ctgactcagg atacagtggt ggctctccaa gccctctcca aatatggatc
     3901 tgccactttt acgagaagtc agaaagaagt gttggtcaca tcgagatctt cagggacctt
     3961 ctctaagact ttccatgtta acagtgggaa tcgcctgctg ctgcaggaag tcaggctgcc
     4021 agatctgccc ggaaattacg tcaccaaagg gtcaggatca ggatgtgtgt accttcagac
     4081 atctctaaag tacaacatcc tcccagtggc agatggaaaa gcacccttcg ctctgcaagt
     4141 caacactctc ccactaaact ttgacaaggc agaggatcac agaacattcc agattcgcat
     4201 caacgtaagc tacactggag aacgacccag ctccaacatg gtcattgttg atgtgaagat
     4261 ggtatcaggc ttcataccta tgaagccatc cgtgaaaagg ctccaagacc agcctaacat
     4321 tcagaggact gaagtgaaca ccaaccatgt tctaatctac attgaaaagc taaccaatca
     4381 aaccctcggt ttctccttcg cggtggaaca agacatccca gtaaagaact taaaaccagc
     4441 ccccataaaa gtctatgatt attatgagac agatgaattc accgttgaag aatacagtgc
     4501 ccctttcagt gatggctctg aacaaggaaa tgcttaaaag tcggaaccta tagatgtcct
     4561 cagaaggact cctccacaca tgaagaacca agaagaaaga cagcaagata aacggaaggg
     4621 aattaaaaaa ccataacatt tatatgttga ataaagttat gacatttaaa aaaa
//