From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human nitric oxide synthase mRNA, complete cds.
Date: 29 Sep 92 10:06:43 GMT
Distribution: bionet
Checksum: 01835     9

LOCUS       HUMNOSSN     4780 bp ss-mRNA            PRI       29-SEP-1992
DEFINITION  Human nitric oxide synthase mRNA, complete cds.
ACCESSION   L02881
KEYWORDS    nitric oxide synthase.
SOURCE      Homo sapiens (library: lambda gt11) adult brain cDNA to mRNA.
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 4780)
  AUTHORS   Nakane,M., Schmidt,H.H., Pollock,J.S., Forstermann,U. and Murad,F.
  TITLE     Molecular cloning and functional expression of nitric oxide
            synthase from human brain
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     5'UTR           1..430
     CDS             431..4732
                     /function="enzyme"
                     /EC_number="1.14.23"
                     /product="nitric oxide synthase"
                     /codon_start=1
                     /translation="MEDHMFGVQQIQPNVISVRLFKRKVGGLGFLVKERVSKPPVIIS
                     DLIRGGAAEQSGLIQAGDIILAVNGRPLVDLSYDSALEVLRGIASETHVVLILRGPEG
                     FTTHLETTFTGDGTPKTIRVTQPLGPPTKAVDLSHQPPAGKEQPLAVDGASGPGNGPQ
                     HAYDDGQEAGSLPHANGWPQAPRQDPAKKATRVSLQGRGENNELLKEIEPVLSLLTSG
                     SRGVKGGAPAKAEMKDMGIQVDRDLDGKSHKPLPLGVENDRVFNDLWGKGNVPVVLNN
                     PYSEKEQPPTSGKQSPTKNGSPSKCPRFLKVKNWETEVVLTDTLHLKSTLETGCTEYI
                     CMGSIMHPSQHARRPEDVRTKGQLFPLAKEFIDQYYSSIKRFGSKAHMERLEEVNKEI
                     DTTSTYQLKDTELIYGAKHAWRNASRCVGRIQWSKLQVFDARDCTTAHGMFNYICNHV
                     KYATNKGNLRSAITIFPQRTDGKHDFRVWNSQLIRYAGYKHRDGSTLGDPANVQFTEI
                     CIQQGWKPPRGRFDVLPLLLQANGNDPELFQIPPELVLELPIRHPKFEWFKDLALKWY
                     GLPAVSNMLLEIGGLEFSACPFSGWYMGTEIGVRDYCDNSRYNILEEVAKKMNLDMRK
                     TSSLWKDQALVEINIAVLYSFQSDKVTIVDHHSATESFIKHMENEYRCRGGCPADWVW
                     IVPPMSGSITPVFHQEMLNYRLTPSFEYQPDPWNTHVWKGTNGTPTKRRAIGFKKLAE
                     AVKFSAKLMGQAMAKRVKATILYATETGKSQAYAKTLCEIFKHAFDAKVMSMEEYDIV
                     HLEHETLVLVVTSTFGNGDPPENGEKFGCALMEMRHPNSVQEERKSYKVRFNSVSSYS
                     DSQKSSGDGPDLRDNFESAGPLANVRFSVFGLGSRAYPHFCAFGHAVDTLLEELGGER
                     ILKMREGDELCGQEEAFRTWAKKVFKAACDVFCVGDDVNIEKANNSLISNDRSWKRNK
                     FRLTFVAEAPELTQGLSNVHKKRVSAARLLSRQNLQSPKSSRSTIFVRLHTNGSQELQ
                     YQPGDHLGVFPGNHEDLVNALIERLEDAPPVNQMVKVELLEERNTALGVISNWTDELR
                     LPPCTIFQAFKYYLDITTPPTPLQLQQFASLATSEKEKQRLLVLSKGLQEYEEWKWGK
                     NPTIVEVLEEFPSIQMPATLLLTQLSLLQPRYYSISSSPDMYPDEVHLTVAIVSYRTR
                     DGEGPIHHGVCSSWLNRIQADELVPCFVRGAPSFHLPRNPQVPCILVGPGTGIAPFRS
                     FWQQRQFDIQHKGMNPCPMVLVFGCRQSKIDHIYREETLQAKNKGVFRELYTAYSREP
                     DKPKKYVQDILQEQLAESVYRALKEQGGHIYVCGDVTMAADVLKAIQRIMTQQGKLSA
                     EDAGVFISRMRDDNRYHEDIFGVTLRTIEVTNRLRSESIAFIEESKKDTDEVFSS"
     3'UTR           4733..4780
BASE COUNT     1159 a   1413 c   1292 g    916 t
ORIGIN
        1 gagcggacgg gctcatgatg cctcagatct gatccgcatc taacaggctg gcaatgaaga
       61 tacccagaga atagttcaca tctatcatgc gtcacttcta gacacagcca tcagacgcat
      121 ctcctcccct ttctgcctga ccttaggaca cgtcccaccg cctctcttga cgtctgcctg
      181 gtcaaccatc acttccttag agaataagga gagaggcgga tgcaggaaat catgccaccg
      241 acgggccacc agccatgagt gggtgacgct gagctgacgt caaagacaga gagggctgaa
      301 gccttgtcag cacctgtcac cccggctcct gctctccgtg tagcctgaag cctggatcct
      361 cctggtgaaa tcatcttggc ctgatagcat tgtgaggtct tcagacagga cccctcggaa
      421 gctagttacc atggaggatc acatgttcgg tgttcagcaa atccagccca atgtcatttc
      481 tgttcgtctc ttcaagcgca aagttggggg cctgggattt ctggtgaagg agcgggtcag
      541 taagccgccc gtgatcatct ctgacctgat tcgtgggggc gccgcagagc agagtggcct
      601 catccaggcc ggagacatca ttcttgcggt caacggccgg cccttggtgg acctgagcta
      661 tgacagcgcc ctggaggtac tcagaggcat tgcctctgag acccacgtgg tcctcattct
      721 gaggggccct gaaggtttca ccacgcacct ggagaccacc tttacaggtg atgggacccc
      781 caagaccatc cgggtgacac agcccctggg tccccccacc aaagccgtgg atctgtccca
      841 ccagccaccg gccggcaaag aacagcccct ggcagtggat ggggcctcgg gtcccgggaa
      901 tgggcctcag catgcctacg atgatgggca ggaggctggc tcactccccc atgccaacgg
      961 ctggccccag gcccccaggc aggaccccgc gaagaaagca accagagtca gcctccaagg
     1021 cagaggggag aacaatgaac tgctcaagga gatagagcct gtgctgagcc ttctcaccag
     1081 tgggagcaga ggggtcaagg gaggggcacc tgccaaggca gagatgaaag atatgggaat
     1141 ccaggtggac agagatttgg acggcaagtc acacaaacct ctgcccctcg gcgtggagaa
     1201 cgaccgagtc ttcaatgacc tatgggggaa gggcaatgtg cctgtcgtcc tcaacaaccc
     1261 atattcagag aaggagcagc cccccacctc aggaaaacag tcccccacaa agaatggcag
     1321 cccctccaag tgtccacgct tcctcaaggt caagaactgg gagactgagg tggttctcac
     1381 tgacaccctc caccttaaga gcacattgga aacgggatgc actgagtaca tctgcatggg
     1441 ctccatcatg catccttctc agcatgcaag gaggcctgaa gacgtccgca caaaaggaca
     1501 gctcttccct ctcgccaaag agtttattga tcaatactat tcatcaatta aaagatttgg
     1561 ctccaaagcc cacatggaaa ggctggaaga ggtgaacaaa gagatcgaca ccactagcac
     1621 ttaccagctc aaggacacag agctcatcta tggggccaag cacgcctggc ggaatgcctc
     1681 gcgctgtgtg ggcaggatcc agtggtccaa gctgcaggta ttcgatgccc gtgactgcac
     1741 cacggcccac gggatgttca actacatctg taaccatgtc aagtatgcca ccaacaaagg
     1801 gaacctcagg tctgccatca ccatattccc ccagaggaca gacggcaagc acgacttccg
     1861 agtctggaac tcccagctca tccgctacgc tggctacaag caccgtgacg gctccaccct
     1921 gggggaccca gccaatgtgc agttcacaga gatatgcata cagcagggct ggaaaccgcc
     1981 tagaggccgc ttcgatgtcc tgccgctcct gcttcaggcc aacggcaatg accctgagct
     2041 cttccagatt cctccagagc tggtgttgga acttcccatc aggcacccca agtttgagtg
     2101 gttcaaggac ctggcgctga agtggtacgg cctccccgcc gtgtccaaca tgctcctaga
     2161 gattggcggc ctggagttca gcgcctgtcc cttcagtggc tggtacatgg gcacagagat
     2221 tggtgtccgc gactactgtg acaactcccg ctacaatatc ctggaggaag tggccaagaa
     2281 gatgaactta gacatgagga agacgtcctc cctgtggaag gaccaggcgc tggtggagat
     2341 caatatcgcg gttctctata gcttccagag tgacaaagtg accattgttg accatcactc
     2401 cgccaccgag tccttcatta agcacatgga gaatgagtac cgctgccggg ggggctgccc
     2461 tgccgactgg gtgtggatcg tgccccccat gtccggaagc atcacccctg tgttccacca
     2521 ggagatgctc aactaccggc tcaccccctc cttcgaatac cagcctgatc cctggaacac
     2581 gcatgtctgg aaaggcacca acgggacccc cacaaagcgg cgagccatcg gcttcaagaa
     2641 gctagcagaa gctgtcaagt tctcggccaa gctgatgggg caggctatgg ccaagagggt
     2701 gaaagcgacc atcctctatg ccacagagac aggcaaatcg caagcttatg ccaagacctt
     2761 gtgtgagatc ttcaaacacg cctttgatgc caaggtgatg tccatggaag aatatgacat
     2821 tgtgcacctg gaacatgaaa ctctggtcct tgtggtcacc agcacctttg gcaatggaga
     2881 tccccctgag aatggggaga aattcggctg tgctttgatg gaaatgaggc accccaactc
     2941 tgtgcaggaa gaaaggaaga gctacaaggt ccgattcaac agcgtctcct cctactctga
     3001 ctcccaaaaa tcatcaggcg atgggcccga cctcagagac aactttgaga gtgctggacc
     3061 cctggccaat gtgaggttct cagtttttgg cctcggctca cgagcatacc ctcacttttg
     3121 cgccttcgga cacgctgtgg acaccctcct ggaagaactg ggaggggaga ggatcctgaa
     3181 gatgagggaa ggggatgagc tctgtgggca ggaagaggct ttcaggacct gggccaagaa
     3241 ggtcttcaag gcagcctgtg atgtcttctg tgtgggagat gatgtcaaca ttgaaaaggc
     3301 caacaattcc ctcatcagca atgatcgcag ctggaagaga aacaagttcc gcctcacctt
     3361 tgtggccgaa gctccagaac tcacacaagg tctatccaat gtccacaaaa agcgagtctc
     3421 agctgcccgg ctccttagcc gtcaaaacct ccagagccct aaatccagtc ggtcaactat
     3481 cttcgtgcgt ctccacacca acgggagcca ggagctgcag taccagcctg gggaccacct
     3541 gggtgtcttc cctggcaacc acgaggacct cgtgaatgcc ctgatcgagc ggctggagga
     3601 cgcgccgcct gtcaaccaga tggtgaaagt ggaactgctg gaggagcgga acacggcttt
     3661 aggtgtcatc agtaactgga cagacgagct ccgcctcccg ccctgcacca tcttccaggc
     3721 cttcaagtac tacctggaca tcaccacgcc accaacgcct ctgcagctgc agcagtttgc
     3781 ctccctagct accagcgaga aggagaagca gcgtctgctg gtcctcagca agggtttgca
     3841 ggagtacgag gaatggaaat ggggcaagaa ccccaccatc gtggaggtgc tggaggagtt
     3901 cccatctatc cagatgccgg ccaccctgct cctgacccag ctgtccctgc tgcagccccg
     3961 ctactattcc atcagctcct ccccagacat gtaccctgat gaagtgcacc tcactgtggc
     4021 catcgtttcc taccgcactc gagatggaga aggaccaatt caccacggcg tatgctcctc
     4081 ctggctcaac cggatacagg ctgacgaact ggtcccctgt ttcgtgagag gagcacccag
     4141 cttccacctg ccccggaacc cccaagtccc ctgcatcctc gttggaccag gcaccggcat
     4201 tgcccctttc cgaagcttct ggcaacagcg gcaatttgat atccaacaca aaggaatgaa
     4261 cccctgcccc atggtcctgg tcttcgggtg ccggcaatcc aagatagatc atatctacag
     4321 ggaagagacc ctgcaggcca agaacaaggg ggtcttcaga gagctgtaca cggcttactc
     4381 ccgggagcca gacaaaccaa agaagtacgt gcaggacatc ctgcaggagc agctggcgga
     4441 gtctgtgtac cgagccctga aggagcaagg gggccacata tacgtctgtg gggacgtcac
     4501 catggctgct gatgtcctca aagccatcca gcgcatcatg acccagcagg ggaagctctc
     4561 ggcagaggac gccggcgtat tcatcagccg gatgagggat gacaaccgat accatgagga
     4621 tatttttgga gtcaccctgc gaacgatcga agtgaccaac cgccttagat ctgagtccat
     4681 tgccttcatt gaagagagca aaaaagacac cgatgaggtt ttcagctcct aactggaccc
     4741 tcttgcccag ccggctgcaa gtttgtaagc gcgggacaga
//