From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Anabaena sp. (clone pAn651) ATPase subunits beta and epsilon (atpB
Date: 15 Sep 92 10:02:03 GMT
Distribution: bionet
Checksum: 38818     6

LOCUS       ANAATPBE     2345 bp ds-DNA             BCT       15-SEP-1992
DEFINITION  Anabaena sp. (clone pAn651) ATPase subunits beta and epsilon (atpB
            and atpE) genes, complete cds.
ACCESSION   M15336
KEYWORDS    ATPase.
SOURCE      Anabaena sp. (library: PCC 7120) DNA.
  ORGANISM  Anabaena sp.
            Prokaryotae; Gracilicutes; Oxyphotobacteria; Cyanobacteria;
            Nostocales; Nostocaceae.
REFERENCE   1  (bases 1 to 2345)
  AUTHORS   Curtis,S.E.
  TITLE     Genes encoding the beta and epsilon subunits of the
            proton-translocating ATPase from Anabaena sp. strain PCC 7120
  JOURNAL   J. Bacteriol. 169, 80-86 (1987)
  STANDARD  full staff_review
COMMENT     The atpB and atpE genes are expressed on an mRNA of about 2.3 kb.
            The endpoints of the two mRNAs have been mapped upstream of the
            atpB gene.  It is not known whether the shorter transcript is
            derived from the longer by processing or degradation, or whether
            the transcripts represent distinct transcription initiation sites.
FEATURES             Location/Qualifiers
     mRNA            105..>2223
                     /note="contains both the atpB and atpE genes (alt.)"
                     /gene="atp"
     mRNA            234..>2223
                     /note="contains both the atpB and atpE genes (alt.)"
                     /gene="atp"
     CDS             301..1716
                     /product="ATPase beta-subunit"
                     /gene="atpB"
                     /codon_start=1
                     /translation="MVTTAEKTNIGYITQIIGPVVDVKFPNGKLPQIYNALTIKGTNE
                     AGQQLNLTVEVQQLLGDNQIRAVAMSSTDGLVRGLEVVDTGAPISVPVGKATLGRIFN
                     VLGEPVDNRGPVNNQETLPIHRPAPKLTELETKPSVFETGIKVVDLLTPYRRGGKIGL
                     FGGAGVGKTVIMMELINNIATQHGGVSVFAGVGERTREGNDLYNEMIDSKNALVYGQM
                     NEPPGARMRVGLSGLTMAEYFRDVNKQDVLLFIDNIFRFVQAGSEVSALLGRMPSAVG
                     YQPTLGTDVGQLQERITSTTEGSITSIQAVYVPADDLTDPAPATTFAHLDGTTVLSRG
                     LASKGIYPAVDPLGSTSTMLQPNIVGDEHYNTARAVQSTLQRYKELQDIIAILGLDEL
                     SEEDRLLVARARKVERFLSQPFFVAEVFTGSPGKYVKLEDTIKGFQKILSGELDDLPE
                     QAFYLVGDINEAIAKAEKIKG"
     CDS             1810..2223
                     /product="ATPase epsilon-subunit"
                     /gene="atpE"
                     /codon_start=1
                     /translation="MTLTVRVISPDKTVWDAEVDEVILPSTTGQLGILSGHAPLSTAL
                     DTGVLRVRTSKSQNWQAIALLGGFAEVEEDEVTILVNGGERGDTINLEEARTAYSQAQ
                     TKLNQVPAGDRQAQIQANQAFKRARARFQATGGLA"
BASE COUNT      653 a    546 c    549 g    597 t
ORIGIN      110 bp upstream of DdeI site.
        1 atagtcgctt cttttttgtt tttctttcga ggctacaaac accatactta agcgcagttc
       61 tctgatgttg taagtcttaa gaatcaattt atgatataaa gttgggtcac tcagtcaagc
      121 tggtgggaat aaagttgggt cactcagtca agctggtggg aataaagtcg ccgaactact
      181 ttttaaggaa ttactaacgg aactctgtcc ccctccggtg ttagagtgaa agatgacatt
      241 gcataatctt atgtcttcta agacccattc tgaaatatag cttcaaccat taggcgcagc
      301 atggtcacca ccgcagaaaa aacaaacatc ggttacatta cccaaatcat tggtccggtt
      361 gtagacgtta aattccctaa cggaaaatta ccgcaaatct acaacgctct gaccatcaaa
      421 ggcactaacg aagctggaca gcaactcaac ctcaccgtag aagtacagca acttttgggc
      481 gacaaccaaa tacgcgccgt tgctatgagt tccacggatg gtttagtccg tggtctggaa
      541 gtcgtcgata caggcgctcc catcagcgta cccgttggta aagcaactct cggacggatt
      601 ttcaacgttc ttggcgaacc tgtagacaac agaggcccag tcaacaacca agaaacccta
      661 cccatccacc gtccggctcc caaattaacc gaactggaaa ctaaaccttc cgttttcgag
      721 accgggatta aagttgttga cctcctcaca ccctatcgac gcggcggtaa aattggtctg
      781 ttcggcggtg cgggtgtcgg caaaaccgta atcatgatgg agttgattaa caacattgca
      841 acccagcatg gtggcgtgtc tgtgttcgct ggcgtgggag agcgtacccg tgaagggaat
      901 gacctctaca acgaaatgat tgattcgaag aatgcgttgg tgtacggtca gatgaacgaa
      961 ccacccggag caagaatgcg ggttggtttg tctggtttga caatggctga atacttccgt
     1021 gatgtcaaca agcaagacgt attgttgttt attgacaaca ttttccggtt cgtacaagca
     1081 ggttccgaag tatcagcgtt gttgggtcgg atgccttctg ctgtgggata tcagccaact
     1141 ttgggtactg atgtaggtca attgcaagaa cggattacat ctactaccga aggttctatt
     1201 acctcaattc aagctgtata tgtacctgcg gatgacttga cagaccccgc acctgcaacc
     1261 acctttgctc acttagatgg tacaactgta ctatctcgtg gcttggcatc taaaggtatt
     1321 tatcctgcgg tagatccgct aggttccacc tctaccatgt tgcaacccaa cattgttggt
     1381 gacgaacact acaatactgc tcgtgctgtc caatcgaccc tgcaacgcta caaagaattg
     1441 caagacatca tcgctattct gggtctagat gaactgtctg aagaagacag actcctcgta
     1501 gcacgggccc ggaaagttga gcgtttcttg tctcaaccct tctttgtcgc tgaagtattc
     1561 acaggttctc ctggtaagta cgtgaagtta gaagacacca tcaaagggtt ccagaagatt
     1621 ctatctggtg agttggatga tctgccagag caagccttct acttggtagg cgacatcaac
     1681 gaagcgatcg ccaaagccga aaaaatcaaa ggctaatttg ttagtagtca gtagtcagta
     1741 gtcagtagat ttgaaggctg acagctgacg actgacaact gacaactgac aattcacaat
     1801 ggaagactta tgaccctaac tgtccgtgta atttccccag ataaaacagt gtgggatgcg
     1861 gaagtcgatg aagtgatttt acccagcact accggtcagc taggtatcct gagtggacac
     1921 gcaccacttt caaccgcact agatacaggc gtactacgag tacgcaccag caagagtcaa
     1981 aattggcaag cgatcgccct attgggtggc tttgccgaag ttgaagaaga tgaagtcacc
     2041 attcttgtca atggtggtga acgcggcgac acgattaacc tagaagaagc acgtactgct
     2101 tacagccaag cgcaaacaaa gctgaatcaa gtacccgcag gcgatcgtca agcccaaatc
     2161 caagctaatc aagcctttaa acgcgcccgc gctcgttttc aagcgactgg cggtttggca
     2221 taaaaaaata caagcagaat tttttagggt gggcaatttg ctcatccttc tttgttttta
     2281 ggatatgcgg ttttggaaca atacggttca gttaagtcta aaatctgggg tttgtgaagt
     2341 aagga
//