From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Phage-resistant plasmid pCI528 (from Lactococcus lactis cremoris)
Date: 16 Dec 92 23:23:51 GMT
Distribution: bionet
Checksum: 02169 5

LOCUS       CI5REPAP     1816 bp ds-DNA             BCT       15-DEC-1992
DEFINITION  Phage-resistant plasmid pCI528 (from Lactococcus lactis cremoris)
            replication-associated protein gene, complete cds. and repeat
            regions.
ACCESSION   L06274
KEYWORDS    replication-associated protein.
SOURCE      Plasmid pCI582 DNA.
  ORGANISM  Plasmid pCI582
            Prokaryotae.
REFERENCE   1  (bases 1 to 1816)
  AUTHORS   Lucey,M.T.
  TITLE     Analysis of the genetic, biochemical and conjugative properties of
            the bacteriophage resistance plasmid pCI528 from L. lactis ssp.
            cremoris UC503
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     repeat_region   one-of(436..446,466..476)
     repeat_region   one-of(387..399,409..421)
     repeat_region   one-of(320..341,342..363,364..385,386..402)
     repeat_unit     one-of(240..249,253..262)
     rep_origin      100..490
     -35_signal      403..408
     -10_signal      427..432
     RBS             494..498
     CDS             508..1659
                     /product="replication-associated protein"
                     /codon_start=1
                     /translation="MSIITEFEKNQKQVKALNELSKRKVVEHNSLITSIAKMDKTPLK
                     MFELAVSCINTEAPPKDHTVYLSKTELFAFFKVSDNDKHSRFKQAVENMQKQAFFKIQ
                     EKKEYGFEFENIVPIPYVKWADYHDEVTIRFSPEIMPYLINLKQNFTQHALSDIAELN
                     SKYSIILYRWLSMNYNQYEHYSAKGGRREEQVETYRNPPISIRELREMTDTMKDYPRF
                     QSLESYIIKNSLKEINEHTSFKVTYEKVKKGRSINSIVFHITKKRRADDNSYKLEDKV
                     YQKAKVQKEQKENLLYAEAMQSKYTKLLLEHFLLSPYEMTNPATMAGLQRNVYPKYDE
                     LKDLMGIDGVKKHLSYIYDKQEPYSKGNIAKYLKKAIEQYLPTVKRRGL"
BASE COUNT      728 a    257 c    308 g    523 t
ORIGIN      
        1 accaatgaat taaatatttg attcaaaagg ctttaaccga tgatttagaa aagaacaatt
       61 ctttaaagga attgatgcga gttattatta cgagggatat tatgaaataa aaacagaaga
      121 tttataaata accgatacaa acagactgat caaattctat attgattagt ctgtttgctt
      181 acgggatgat tttgactttt gattttaaat ttttgaaaaa aataaaaaaa gcgaagccta
      241 tattaattta tcatatatat tttaatcttt tcttcttttg cgtcaaaaaa aagttagtat
      301 ttttaagggg ttacagaata atatagcata aaaaaactgt gtatatagca taaaaaaact
      361 gtgtatatag cataaaaaaa ctgtgtatat agcataaaaa aatcatcatt ttatgctata
      421 ttatgatata ataaaagtat gaagaacaaa cttttgaacg agaatttctt catacttact
      481 tatgaacacg cagaggagcg tatctttatg tctattataa cagaatttga aaaaaatcag
      541 aagcaggtga aagccttgaa tgaattgtca aagcgtaaag tagtcgagca taactcttta
      601 attaccagca ttgcgaaaat ggataaaaca ccacttaaaa tgtttgagtt agcggtatca
      661 tgtattaata ccgaggctcc tcctaaagat catacggttt acctctcaaa aaccgaactt
      721 tttgcgttct ttaaagtttc tgacaatgac aaacatagtc gtttcaagca ggcagttgag
      781 aatatgcaaa aacaagcctt ctttaaaatc caagaaaaaa aagaatatgg gttcgagttt
      841 gaaaatattg tgccgattcc ctatgtaaaa tgggctgatt atcatgatga agttactatc
      901 aggtttagtc ctgaaattat gccttattta atcaatctaa aacaaaattt cacgcaacat
      961 gccttgtccg atatagcaga actgaatagc aagtacagca ttatcttgta tcgatggcta
     1021 tctatgaatt acaaccagta cgagcattac agtgccaaag gcggacgccg agaagaacaa
     1081 gtggaaacct atcgcaatcc gccaatttct atacgagaat tgcgagaaat gacagataca
     1141 atgaaggatt atcctcgttt tcaaagttta gaaagctaca taataaagaa ttctttgaaa
     1201 gaaattaatg agcatacctc ttttaaagtg acttatgaga aagtcaaaaa aggaagaagc
     1261 attaattcta ttgtttttca tatcactaaa aaacgaagag cagatgataa tagctacaag
     1321 ttagaagata aagtatatca aaaagcgaaa gttcaaaaag aacaaaaaga aaatttatta
     1381 tatgcagaag ctatgcaaag caaatacacc aagttattgc tggaacattt tcttttatca
     1441 ccttatgaaa tgacaaatcc agctactatg gctggattgc agagaaatgt ttatccaaaa
     1501 tatgatgaac tcaaagattt aatgggtata gatggagtta aaaaacacct ttcatacatt
     1561 tacgataaac aagaaccgta ttctaaagga aatattgcaa agtatcttaa aaaagccatt
     1621 gagcaatacc taccaacggt taaaaggcgg ggattataat gagtgataat ttaaaaacca
     1681 taaaggagct agcggacgag ttaggcgtta gtaagacagc tataaacaaa aaggtcacag
     1741 atagagaacg aaaactttgg ttttcgaaaa taggaaacaa gtttgtgatt aatgaagatg
     1801 gtcaaaaatc aataaa
//