From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: morphogene cluster: spoVE=mra homolog required for spore cortex
Date: 6 Jan 93 07:00:02 GMT
Distribution: bionet
Checksum: 42422 5

LOCUS       M257759S2    1400 bp    DNA             BCT       27-DEC-1992
DEFINITION  morphogene cluster: spoVE=mra homolog required for spore cortex
            formation...orf2 3' of murG [Bacillus subtilis, Genomic, 5 genes,
            1400 nt, segment 2 of 2] 
ACCESSION   S46399
KEYWORDS    .
SEGMENT     2 of 2
SOURCE      Bacillus subtilis
  ORGANISM  Bacillus subtilis
            Unclassified.
REFERENCE   1  (bases 1 to 1400)
  AUTHORS   Henriques,A.O., de Lencastre,H. and Piggot,P.J.
  TITLE     A Bacillus subtilis morphogene cluster that includes spoVE is
            homologous to the mra region of Escherichia coli.
  JOURNAL   Biochimie 74, 735-748 (1992)
  STANDARD  full automatic
COMMENT     This entry [NCBI gibbsq 116436] was created by the journal scanning
            component of NCBI/GenBank at the National Library of Medicine.
            This sequence comes from Fig. 3.
            Region: morphogene cluster.  
FEATURES             Location/Qualifiers
     CDS             3..95
                     /partial
                     /note="initial Thr codon incomplete.  mra homolog required
                     for spore cortex formation;  For the protein sequence
                     (NCBI gibbsq 116435):  Method: conceptual translation
                     supplied by author.  This sequence comes from Fig. 3.
                     Author-given protein sequence is in conflict with the
                     conceptual translation"
                     /gene="spoVE"
                     /product="SpoVE"
                     /codon_start=1
                     /translation="TLPFLSYGGSSLTLMLMAVGVLLNVSRYSRY"
     CDS             218..1309
                     /note="MurG;  peptidoglycan biosynthesis;  For the protein
                     sequence (NCBI gibbsq 116437):  Method: conceptual
                     translation supplied by author.  This sequence comes from
                     Fig. 3."
                     /gene="murG"
                     /product="UDP-N-acetylglucosamine:N-acetylmuramyl-(pentape
                     ptide) pyrophosphoryl-undecaprenol N-acetylglucosamine
                     transferase"
                     /codon_start=1
                     /translation="MRIAISGGGTGGHIYPALAFIKEVQRRHPNVEFLYIGTENGLEK
                     KIVERENIPFRSIEITGFKRKLSFENVKTVMRFLKGVKKSKSYLAEFKPDAVIGTGGY
                     VCGPVVYAAAKMGIPTIVHEQNSLPGITNKFLSKYVNKVAICFEEAKSHFPSEKVVFT
                     GNPRASEVVSIKTGRSLAEFKLSEDKKTVLIFGGSRGAAPINRAVIDMQDVLKTRDYQ
                     VLYITGEVHYEKVMNELKSKGAADNMVTKPFLHQMPEYLKAIDVIVARAGAATIAEIT
                     ALGIPSVLIPSPYVTANHQEVNARSLGQHDAAIVLKETELSGEKLIEALDRIVLNEQT
                     LKEMSERTKSLGVPDAAARLYSVLEELKK"
     CDS             1336..1400
                     /partial
                     /note="Description: orf2 3' of murG;  For the protein
                     sequence (NCBI gibbsq 116438):  Method: conceptual
                     translation supplied by author.  This sequence comes from
                     Fig. 3."
                     /codon_start=1
                     /translation="MEKVIQELKEREVGKVLANEPL"
BASE COUNT      441 a    258 c    354 g    347 t
ORIGIN      
        1 cgctcccgtt tctgagctac ggaggctctt cattgactct aatgctgatg gcagttggtg
       61 tgctgctgaa tgtaagccgc tactcgaggt attaacgaat gtatttccaa gcctcctgtc
      121 taacatgaag cttggaaaca attgatgcga taaccctgtc caacaaaaca gggttatcgt
      181 tatgttatag agaaacttag actgggggaa aaaagaaatg cgaattgcaa taagcggagg
      241 cggtacaggg ggacatattt atcctgctct tgcttttatt aaagaagtgc aaagacgtca
      301 tcctaatgtg gaatttttat atatcggaac agaaaacggg ctggagaaaa aaattgttga
      361 gcgggagaat ataccgtttc gttcaattga aattacagga tttaaaagaa aattgtcatt
      421 tgaaaatgtc aagactgtga tgcgttttct gaagggcgtc aaaaagagta aatcgtattt
      481 agcggaattt aagcccgatg ccgtaattgg aacgggcggt tatgtatgtg ggccggttgt
      541 ctatgccgct gcgaaaatgg gaattccgac tattgtccac gaacaaaaca gcctgccggg
      601 catcacgaat aagtttcttt caaaatatgt taataaagta gcgatttgtt ttgaagaagc
      661 gaaatcacat tttcctagcg agaaagttgt atttacggga aacccgagag cctctgaagt
      721 cgtctcaata aagacgggac gatcattggc tgaatttaaa ctttctgaag acaaaaaaac
      781 ggtcttaatt ttcggcggaa gccgaggcgc tgcaccgatt aaccgcgctg tcattgacat
      841 gcaggatgtg ctgaaaacaa gagactatca agtgctgtat atcaccggtg aagttcacta
      901 tgaaaaagta atgaatgagc tgaaaagcaa gggtgccgct gataatatgg tcacaaagcc
      961 gtttcttcat caaatgcctg agtatttaaa ggccattgat gtcattgtgg ccagagccgg
     1021 tgcagcaacg attgccgaaa ttactgctct cggaatccca agtgttctga tcccaagtcc
     1081 atatgtgaca gcgaatcacc aggaggtcaa tgcgaggtct cttggccagc atgatgcggc
     1141 gattgtactg aaagaaacag agctcagcgg ggagaagctg attgaagcgc tagaccggat
     1201 tgtactgaat gagcaaacgt taaaggaaat gagcgaacga acaaagtcac tcggtgttcc
     1261 tgacgcggct gcccgtttat acagcgtact cgaggaatta aaaaaataga aaagcaaatg
     1321 gaatgcgagg ttacgatgga gaaagtgata caggaattaa aagaacgaga agtcggcaag
     1381 gttcttgcaa atgaaccgct
//