From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Z.mobilis gnl gene for gluconolactonase
Date: 15 Aug 92 10:03:43 GMT
Distribution: bionet
Checksum: 54162     4

LOCUS       ZMOGNLG      1180 bp ds-DNA             BCT       15-AUG-1992
DEFINITION  Z.mobilis gnl gene for gluconolactonase
ACCESSION   X67189
KEYWORDS    gluconolactonase.
SOURCE      Zymomonas mobilis DNA.
  ORGANISM  Zymomonas mobilis
            Prokaryotae; Facultatively anaerobic gram-negative rods.
REFERENCE   1  (bases 1 to 1180)
  AUTHORS   Kanagasundaram,S.V. and Scopes,R.K.
  TITLE     Isolation and characterization of the gene encoding
            gluconolactonase from Zymomonase mobilis
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
COMMENT     *source: strain=ZM6 (ATCC 29191); *source: clone=pZVKL1;
            From EMBL    entry ZMGNLG;  dated 04-AUG-1992.
FEATURES             Location/Qualifiers
     RBS             115..118
     sig_peptide     127..231
     CDS             127..1089
                     /EC_number="3.1.1.17"
                     /product="gluconolactonase"
                     /gene="gnl"
                     /codon_start=1
                     /translation="MTTGRMSRRECLSAAVMVPIAAMTATATITGSAEAAKNNMNGST
                     IGKITKFSPRLDAILDVSTPIEVIASDIQWSEGPVWVKNGNFLLFSDPPANIMRKWTP
                     DAGVSIFLKPSGHAEPIPAGQFREPGSNGMKVGPDGKIWVADSGTRAIMKVDPVTRQR
                     SVVDNYKGKRFNSPNDLFFSKSGAVYFTDPPYGLTNLDESDIKEMNYNGVFRLSPDGR
                     LDLIEAGLSRPNGLALSPDETKLYVSNSDRASPNIWVYSLDSNGLPTSRTLLRNFRKE
                     YFDQGLAGLPDGMNIDKQGNLFASAPGGIYIFAPDGECLGLIFW"
     mat_peptide     232..1086
                     /EC_number="3.1.1.17"
                     /product="gluconolactonase"
     terminator      1123..1145
BASE COUNT      279 a    284 c    269 g    348 t
ORIGIN
        1 actttttttc aaacttattt tttgagacgt taactatcgt gatttttctt taatttttaa
       61 gaaaaataac aagacttttt atgacctttt ttgaataaag atgcggttag ttgcaggagt
      121 cgtaaaatga ccactggtcg tatgtctcgt cgagaatgcc tttccgcagc tgttatggtg
      181 cctatcgccg ctatgacagc aaccgccact attacaggat cagctgaggc tgcaaagaat
      241 aatatgaacg gatctaccat cggtaagatt accaaatttt cacctcgcct tgatgctatt
      301 ttagatgtct ctaccccaat tgaggtcatt gcatctgata ttcaatggtc agaaggccct
      361 gtatgggtca aaaatggcaa tttcctgctg tttagtgatc cgcccgccaa tattatgcgt
      421 aaatggacgc ctgatgccgg tgtcagcatc ttcctgaaac cttcaggtca tgccgaaccc
      481 attccggccg gtcagttccg tgaaccgggt tccaacggta tgaaagttgg ccccgatggc
      541 aaaatctggg ttgcagacag tggcacgcgc gcgatcatga aagtggatcc ggtcacccgt
      601 cagcgcagtg ttgtcgataa ctataaaggc aaacgcttta acagcccgaa tgacctcttc
      661 ttctcgaagt caggcgctgt ttatttcacc gatccgccct atggtctgac caatcttgat
      721 gaatccgaca tcaaagagat gaactacaat ggtgtgttcc gtctctctcc cgatggtcgt
      781 ttagatttga tcgaagctgg tcttagccgc ccgaatggtt tggctctgtc cccggatgaa
      841 acgaaacttt atgtttctaa ctcagaccgc gccagcccga atatctgggt ttattctttg
      901 gatagcaatg gcttgccaac cagccggact ttgctgcgga acttccgcaa ggaatatttc
      961 gatcagggtc tcgccggtct tcctgatggt atgaatatcg acaaacaggg caatcttttt
     1021 gcttctgctc cgggcggtat ctatattttt gcaccagatg gcgaatgctt gggtcttatt
     1081 ttctggtaac ccaagtcagc cgttgtcgaa ctgctgcttt ggtgaaaaag gccagacctt
     1141 gttcatctcg gcttctcaca ttgttgtgcc gttcgcacca
//