From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Saccharomyces cerevisiae kinesin-related protein (CIN8) gene,
Date: 15 Sep 92 10:06:22 GMT
Distribution: bionet
Checksum: 20166     8

LOCUS       YSCCIN8A     3884 bp ds-DNA             PLN       15-SEP-1992
DEFINITION  Saccharomyces cerevisiae kinesin-related protein (CIN8) gene,
            complete cds, and protease B (PRB1) gene, 3' end.
ACCESSION   M90522
KEYWORDS    CIN8 gene; kinesin-related protein.
SOURCE      Saccharomyces cerevisiae (strain S288C) DNA.
  ORGANISM  Saccharomyces cerevisiae
            Eukaryota; Fungi; Eumycota; Hemiascomycetes; Endomycetales;
            Saccharomycetaceae.
REFERENCE   1  (bases 1 to 3884)
  AUTHORS   Moehle,C.M., Tizard,R., Lemmon,S.K., Smart,J. and Jones,E.W.
  TITLE     Protease B of the lysosomelike vacuole of the yeast Saccharomyces
            cerevisiae is homologous to the subtilisin family of serine
            proteases
  JOURNAL   Mol. Cell. Biol. 7, 4390-4399 (1987)
  STANDARD  full staff_review
REFERENCE   2  (bases 1 to 3884)
  AUTHORS   Saunders,W.S., He,L., Loo,K.K. and Hoyt,M.A.
  TITLE     Two Saccharomyces cerevisiae kinesin-related gene products required
            for mitotic spindle assembly
  JOURNAL   J. Cell Biol. 118, 109-120 (1992)
  STANDARD  full staff_entry
FEATURES             Location/Qualifiers
     CDS             <1..96
                     /product="protease B"
                     /gene="PRB1"
                     /codon_start=1
                     /translation="KQELNMDEFIGSKTDLIKDQVRDILDKLNII"
                     /citation=[1]
     CDS             491..3607
                     /function="mitotic spindle assembly and stabilization"
                     /product="kinesin-related protein"
                     /gene="CIN8"
                     /codon_start=1
                     /translation="MVWPESNVEYRYRSKQFKGKSNKKYIKAQKIQQERICLMPAENQ
                     NTGQDRSSNSISKNGNSQVGCHTVPNEELNITVAVRCRGRNEREISMKSSVVVNVPDI
                     TGSKEISINTTGDTGITAQMNAKRYTVDKVFGPGASQDLIFDEVAGPLFQDFIKGYNC
                     TVLVYGMTSTGKTYTMTGDEKLYNGELSDAAGIIPRVLLKLFDTLELQQNDYVVKCSF
                     IELYNEELKDLLDSNSNGSSNTGFDGQFMKKLRIFASSTANNTTSNSASSSRSNSRNS
                     SPRSLNDLTPKAALLRKRLRTKSLPNTIKQQYQQQQAVNSRNNSSSNSGSTTNNASSN
                     TNTNNGQRSSMAPNDQTNGIYIQNLQEFHITNAMEGLNLLQKGLKHRQVASTKMNDFS
                     SRSHTIFTITLYKKHQDELFRISKMNLVDLAGSENINRSGALNQRAKEAGSINQSLLT
                     LGRVINALVDKSGHIPFRESKLTRLLQDSLGGNTKTALIATISPAKVTSEETCSTLEY
                     ASKAKNIKNKPQLGSFIMKDILVKNITMELAKIKSDLLSTKSKEGIYMSQDHYKNLNS
                     DLESYKNEVQECKREIESLTSKNALLVKDKLKSKETIQSQNCQIESLKTTIDHLRAQL
                     DKQHKTEIEISDFNNKLQKLTEVMQMALHDYKKRELDLNQKFEMHITKEIKKLKSTLF
                     LQLNTMQQESILQETNIQPNLDMIKNEVLTLMRTMQEKAELMYKDCVKKILNESPKFF
                     NVVIEKIDIIRVDFQKFYKNIAENLSDISEENNNMKQYLKNHFFKNNHQELLNRHVDS
                     TYENIEKRTNEFVENFKKVLNDHLDENKKLIMHNLTTATSAVIDQEMDLFEPKRVKWE
                     NSFDLINDCDSMNNEFYNSMAATLSQIKSTVDTSSNSMNESISVMKGQVEESENAISL
                     LKNNTKFNDQFEQLINKHNMLKDNIKNSITSTHSHITNVDDIYNTIENIMKNYGNKEN
                     ATKDEMIENILKEIPNLSKKMPLRLSNINSNSVQSVISPKKHAIEDENKSSENVDNEG
                     SRKMLKIE"
                     /citation=[2]
BASE COUNT     1496 a    652 c    680 g   1056 t
ORIGIN
        1 aagcaagaat tgaacatgga tgaattcatt ggatcaaaga ccgatttaat caaagatcaa
       61 gtgagagata ttcttgataa attgaatatt atttaattct tcatttagaa aaatttcagc
      121 tgcttttttt tttctttttc tttccttagg cgtctcgagg ttacaagtcg gagtccctct
      181 tcactatcgt ttgtccactt tttttatatc cccattattt tcaatctgaa tttcattttt
      241 tttttttaat tcatgaaatt tatatgtccc acgtattact acatatttgc gtttttaatt
      301 aaataaataa ctgttacttt tattatatct tatttgcaga tcacttatct gatcaaatgt
      361 tttcgttttc gtgtgtggtg acgatgtatt aggtacgcga aataaacaaa acaaacaaac
      421 aaggccgcaa caataacatc atctaaagac ttcctttgtg acccgcttct caacagcggg
      481 tgtagaactt atggtatggc cagaaagtaa cgttgagtat agatacagaa gcaagcaatt
      541 caaaggaaaa agtaataaaa agtatataaa agcgcaaaaa atacaacaag aaagaatttg
      601 tttgatgcca gcggaaaacc aaaatacggg tcaagataga agctccaaca gcatcagtaa
      661 aaatggcaac tctcaggttg gatgtcacac tgttcctaat gaggaactga acatcactgt
      721 agctgtgcga tgcagaggaa ggaatgaaag ggaaattagt atgaaaagct ccgttgtggt
      781 aaatgttcca gatattacag gttctaaaga aatttccatt aacacgacgg gagataccgg
      841 tataactgct caaatgaatg ccaagagata cacagtggac aaagtcttcg gtcccggcgc
      901 ttcccaggat ctaatttttg atgaagtggc gggcccatta ttccaggatt tcattaaagg
      961 ttacaattgc accgtactgg tatatggtat gacgtcaaca ggtaaaacat atacaatgac
     1021 gggcgacgaa aagttatata atggtgaatt gagcgatgca gcaggaatta taccgagggt
     1081 tcttttgaag ttgtttgaca cattggaact acaacagaac gattacgtag taaaatgttc
     1141 gttcattgaa ctctacaacg aagaattgaa ggacctcttg gacagcaata gcaacggctc
     1201 tagtaatact ggctttgacg gccaatttat gaaaaaattg aggatttttg cttcaagcac
     1261 agcaaataat accactagca acagtgctag tagttccagg agtaattcta ggaacagttc
     1321 tccgaggtca ttaaatgatc taacacctaa agctgctcta ttaagaaaaa ggttaaggac
     1381 aaaatcactg ccgaatacca tcaagcaaca gtatcaacaa caacaggcag tgaattccag
     1441 gaacaactct tcctctaact ctggctctac cactaataat gcttctagta acaccaacac
     1501 aaataacggt caaagaagtt cgatggctcc aaatgaccaa actaatggta tatacatcca
     1561 gaatttgcaa gaatttcaca taacaaatgc tatggagggg ctaaacctat tacaaaaagg
     1621 cttaaagcat aggcaagtag cgtccactaa aatgaacgat ttttccagta gatctcatac
     1681 catttttaca atcactttgt ataagaagca tcaggatgaa ctatttagaa tttccaaaat
     1741 gaatcttgtg gatttagctg gttcagaaaa catcaacaga tccggagcat taaatcaacg
     1801 tgccaaagaa gctggttcaa tcaaccaaag tctattgacg ctgggcaggg tcataaacgc
     1861 actcgtagat aaaagcggcc atataccttt ccgtgaatcg aaattgaccc gcctgcttca
     1921 agattccctg ggtggtaata cgaaaaccgc actaattgct actatatcgc ctgcaaaggt
     1981 aacttctgaa gaaacctgca gtacattaga gtatgcttcg aaggctaaaa acattaagaa
     2041 caagccgcaa ctgggttcat ttataatgaa ggatattttg gttaaaaata taactatgga
     2101 attagcaaag attaaatccg atttactctc tacaaagtcc aaagaaggaa tatatatgag
     2161 ccaagatcac tacaaaaatt tgaacagtga tttagaaagt tataaaaatg aagttcaaga
     2221 atgtaaaaga gaaattgaaa gtttgacatc gaaaaatgca ttgctagtaa aagataaatt
     2281 gaagtcaaaa gaaactattc aatctcaaaa ttgccaaata gaatcattga aaactaccat
     2341 agatcattta agggcacaac tagataaaca gcataaaact gaaattgaaa tatccgattt
     2401 taataacaaa ctacagaagt tgactgaggt aatgcaaatg gccctacatg attacaaaaa
     2461 aagagaactt gaccttaatc aaaagtttga aatgcatatt actaaagaaa ttaaaaaatt
     2521 gaaatctaca ctgtttttac aattaaacac tatgcaacag gaaagtattc ttcaagagac
     2581 taatatccaa ccaaatcttg atatgatcaa aaatgaagta ctgactctta tgagaaccat
     2641 gcaagaaaaa gctgaactaa tgtacaaaga ctgtgtgaag aaaattttaa acgaatctcc
     2701 taaattcttc aatgttgtta ttgagaaaat cgacataata agagtagatt tccaaaaatt
     2761 ttataaaaat atagccgaga atctttctga tattagcgaa gaaaataaca acatgaaaca
     2821 gtacttaaaa aaccattttt tcaagaataa ccatcaagaa ttactgaatc gtcatgtgga
     2881 ttctacttat gaaaatattg agaagagaac aaacgagttt gttgagaact ttaaaaaggt
     2941 cctaaatgac caccttgacg aaaataaaaa actaataatg cacaatctga caactgcaac
     3001 cagcgcggtt attgatcaag aaatggatct gtttgaaccc aagcgcgtta aatgggaaaa
     3061 ttcatttgat ctgataaatg attgtgactc catgaataac gaattctata atagcatggc
     3121 agcgacgcta tcgcaaatca agagtactgt tgatacatca tcaaattcga tgaatgagtc
     3181 tatttcagtc atgaaaggac aagtggaaga atcggagaac gctatatccc ttttgaagaa
     3241 caataccaaa tttaatgatc aatttgagca gcttattaac aagcataaca tgttgaaaga
     3301 taacattaaa aattcgataa catcaacaca ctctcatata actaatgtgg atgatatcta
     3361 taatacgatt gaaaacataa tgaaaaacta tggtaacaag gaaaacgcta ccaaagacga
     3421 aatgatcgag aacatattga aggaaatacc aaatctaagt aagaaaatgc cgttaaggtt
     3481 atcaaacata aatagcaatt cagtgcaaag tgtaatatcg cccaaaaagc atgcaattga
     3541 agatgaaaac aaatccagtg aaaatgtgga caatgagggc tcgagaaaaa tgttaaagat
     3601 tgaatagttg atattgcctt tcagtcgaat atatattcaa actagtggtt aataaaaaca
     3661 aagtatgtaa agaatactca gttattcatt agaaggcaag acagaagaga agggtgtgaa
     3721 accacctcta ccaaacacac caagagatga acctaaatca aattttcaca gagctaacta
     3781 tataaacgtt tggattcgtg tgtactatct ttatttacgg aaataagttg taatattaaa
     3841 aaaaaaaaaa aacattttga tggacaatga atttctctaa tttt
//