HDTenEightyP/NetNews huggingface dataset:
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Lentinus ponderosus ribosomal RNA (17LR0R) large subunit, partial.
Date: 19 Aug 92 10:01:43 GMT
Distribution: bionet
Checksum: 48169     2

LOCUS       LENRRLSAO     232 bp ss-rRNA            RNA       19-AUG-1992
DEFINITION  Lentinus ponderosus ribosomal RNA (17LR0R) large subunit, partial.
ACCESSION   M98647
KEYWORDS    large subunit ribosomal RNA.
SOURCE      Lentinus ponderosus (library: Duke culture collection 612) cDNA to
            rRNA.
  ORGANISM  Lentinus ponderosus
            Eukaryota; Fungi; Eumycota; Basidiomycotina; Hymenomycetes;
            Agaricales; Tricholomataceae.
REFERENCE   1  (bases 1 to 232)
  AUTHORS   Hibbett,D.S. and Vilgalys,R.
  TITLE     Phylogenetic relationships of the basidiomycete genus Lentinus
            inferred from molecular and morphological characters
  JOURNAL   Unpublished (1992)
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     rRNA            <1..>232
                     /note="Aligns to region between positions 70 to 306
                     inSaccharomyces cerevisiae large subunit nuclear rRNA."
                     /product="large subunit ribosomal RNA"
                     /citation=[1]
BASE COUNT       57 a     48 c     69 g     58 t
ORIGIN
        1 cccctagtaa ctgcgagtga agcgggaaga gctcaaattt aaaatctggc ggtcctttgg
       61 ccgtccgagt tgtattctgg agaagtgctt tccgcgctgg accgtgtata agtctcttgg
      121 aacagagcgt catagagggt gagaatcccg tcaaagacac ggactaccag tgctttgtga
      181 tgcgctctca aagagtcgag ttgtttggga atgcagctca aaatgggtgg ta
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human chromosome 4 sequence-tagged site STS4-334
Date: 21 Aug 92 10:09:04 GMT
Distribution: bionet
Checksum: 61077     3

LOCUS       HUM4STS334    459 bp ds-DNA             PRI       21-AUG-1992
DEFINITION  Human chromosome 4 sequence-tagged site STS4-334
ACCESSION   L00809
KEYWORDS    STS; human chromosome 4; sequence tagged site.
SOURCE      Homo sapiens, clone p4-239 from a chromosome 4 plasmid library;
            vector pBluescript II KS + (Stratagene)
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 459)
  AUTHORS   Goold,R.D., diSibio,G., Dugaiczyk,A., Smith,K.A., Xu,H.,
            Magrane,G., Lang,D.B., Cox,D.R., Masters,S.B. and Myers,R.M.
  TITLE     Sequence-Tagged Sites for Human Chromosome 4
  JOURNAL   Unpublished (1992) see COMMENT for author address
  STANDARD  full automatic
COMMENT     PCR components: 25 ng of human genomic DNA, 10 pmol of each
            oligonucleotide, 200 micro-M dNTPs, 0.25 U Taq  polymerase (Cetus)
            in 10 micro-l of 50 mM KCl-20 mM  Tris-HCl, pH 8.3 (at room temp),
            2.5 mM MgCl-2.  Initial denaturation at 94degC for 1.5 min, then 30
            cycles of  94degC for 15 sec, 62degC for 23 sec, and 72degC for 30
            sec, followed by a final extension at 72degC for 3.5 min, using a
            Perkin-Elmer 9600 thermocycler. PCR-amplified product size 202 bp.
            Sequence submitted by:
            Human Genome Mapping Center
            Box 0925
            University of California San Francisco
            San Francisco, CA 94143-0925  USA
            Phone: (415) 502-1612   Fax: (415) 476-8391
            e-mail: hgmcprobes@cgl.ucsf.edu
FEATURES             Location/Qualifiers
     misc_feature    66..89
                     /label=Primer1
     misc_feature    complement(243..267)
                     /label=Primer2
BASE COUNT      150 a    104 c     99 g    102 t      4 others
ORIGIN
        1 ttcctgcagc ccgggggtat ctgcctggag tcagagggac cagtgaggag actattgcaa
       61 agtccaggta ggaaatgcta gaagtctgaa ttggagtgct ggcactgtga catagagaga
      121 tagacatatt caacagagat tcaggggtaa accagatgac tcaacgtttg gagtagttga
      181 aaatgactgc ctgtttctca ggtaggtagc tgatggatac atatgtattt gtttgcattt
      241 aaaacatgct catgtctttc catttgaatt ctaaatagaa aaaaatatag cagcttgcaa
      301 actgtcactc aaaagccgta acacaaaaaa cagatttcaa gcactgaaag ggatgtcatc
      361 actcanncac acacacacac acacacacac acacacacac acacacacac acannctcca
      421 cttgaggatc cactagttct agagcggccg ccaccgcgg
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Gadus ogac (Greenland codfish) mitochondrial cytochrome b gene,
Date: 13 Aug 92 10:00:26 GMT
Distribution: bionet
Checksum: 52600     2

LOCUS       GADMTCYTBM    307 bp ds-DNA             ORG       13-AUG-1992
DEFINITION  Gadus ogac (Greenland codfish) mitochondrial cytochrome b gene,
            partial cds.
ACCESSION   M98550
KEYWORDS    cytochrome b.
SOURCE      Mitochondrion Gadus ogac (chromosome Mitochondrion Gadus ogac)
            heart DNA.
  ORGANISM  Mitochondrion Gadus ogac
            Eukaryota; Animalia; Chordata; Vertebrata; Osteichthyes;
            Actinopterygii; Gadiformes; Gadoidea; Gadidae.
REFERENCE   1  (bases 1 to 307)
  AUTHORS   Carr,S.M. and Marshall,H.D.
  TITLE     A direct approach to the measurement of genetic variation in fish
            populations: Applications of the polymerase chain reaction to
            studies of Atlantic cod, Gadus morhua L.
  JOURNAL   J. Fish Biol. 39, 101-107 (1991)
  STANDARD  full staff_entry
FEATURES             Location/Qualifiers
     CDS             <1..>307
                     /product="cytochrome b"
                     /codon_start=3
                     /translation="FGSLLGLCLITQLLTGLFLAMHYTSDIETAFSSVVHICRDVNYG
                     WLIRNMHANGASFFFICLYMHIARGLYYGSYLFVETWNIGVVLFLLVMMTSFVGYVLP
                     "
BASE COUNT       71 a     72 c     46 g    118 t
ORIGIN
        1 attttggctc tcttctaggc ctttgcttaa ttactcaact tctaacagga ctatttctag
       61 ctatacacta cacctcagac atcgagacag ccttctcatc cgtagtccac atctgtcgtg
      121 atgtaaatta cggttgactt attcggaata tacatgctaa cggtgcctct ttctttttta
      181 tttgtctcta tatacatatt gcccgaggtc tctattatgg ctcttatctt tttgtagaaa
      241 catgaaacat cggagttgtc cttttccttt tagtaataat aacctctttc gtaggttatg
      301 tcctccc
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human chromosome 4 sequence-tagged site STS4-366
Date: 21 Aug 92 10:09:31 GMT
Distribution: bionet
Checksum: 41678     3

LOCUS       HUM4STS366    428 bp ds-DNA             PRI       21-AUG-1992
DEFINITION  Human chromosome 4 sequence-tagged site STS4-366
ACCESSION   L00821
KEYWORDS    STS; human chromosome 4; sequence tagged site.
SOURCE      Homo sapiens, clone p4-282 from a chromosome 4 plasmid library;
            vector pBluescript II KS + (Stratagene)
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 428)
  AUTHORS   Goold,R.D., diSibio,G., Dugaiczyk,A., Smith,K.A., Xu,H.,
            Magrane,G., Lang,D.B., Cox,D.R., Masters,S.B. and Myers,R.M.
  TITLE     Sequence-Tagged Sites for Human Chromosome 4
  JOURNAL   Unpublished (1992) see COMMENT for author address
  STANDARD  full automatic
COMMENT     PCR components: 25 ng of human genomic DNA, 10 pmol of each
            oligonucleotide, 200 micro-M dNTPs, 0.25 U Taq  polymerase (Cetus)
            in 10 micro-l of 50 mM KCl-20 mM  Tris-HCl, pH 8.3 (at room temp),
            2.5 mM MgCl-2.  Initial denaturation at 94degC for 1.5 min, then 30
            cycles of  94degC for 15 sec, 62degC for 23 sec, and 72degC for 30
            sec, followed by a final extension at 72degC for 3.5 min, using a
            Perkin-Elmer 9600 thermocycler. PCR-amplified product size 201 bp.
            STS sequence contains a simple sequence tandem repeat (CA).
            Sequence submitted by:
            Human Genome Mapping Center
            Box 0925
            University of California San Francisco
            San Francisco, CA 94143-0925  USA
            Phone: (415) 502-1612   Fax: (415) 476-8391
            e-mail: hgmcprobes@cgl.ucsf.edu
FEATURES             Location/Qualifiers
     misc_feature    7..30
                     /label=Primer1
     misc_feature    complement(183..207)
                     /label=Primer2
BASE COUNT      142 a    106 c     63 g    112 t      5 others
ORIGIN
        1 gatccctctt caaatccaac ctccacaaca ccacatgcac acacacacac acacacacac
       61 acacacacac agaggcacag acagacacac acacacacat aggcacatgc acatgcagag
      121 tgaacttact tttagagtcc tcaactcaaa tggaacttgc ctagggtggn ccaccttaac
      181 ctcaagacta gaacagattc catcttatac actctcacag cacaccttgt tttttattta
      241 cagcacttac ttcagtntgc aattagaaaa tatttgtata atagctgatg ttccccttta
      301 agctataaga tacattaggt tggggctata tgaatttcgn gcactattgt ntttgataca
      361 ctcagcatag tgtctattac taaggaaatg cagcataaaa nttgttttct gaaggttaaa
      421 ggatacat
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human chromosome 4 sequence-tagged site STS4-236
Date: 21 Aug 92 10:08:00 GMT
Distribution: bionet
Checksum: 32377     3

LOCUS       HUM4STS236    486 bp ds-DNA             PRI       21-AUG-1992
DEFINITION  Human chromosome 4 sequence-tagged site STS4-236
ACCESSION   L00751
KEYWORDS    STS; human chromosome 4; sequence tagged site.
SOURCE      Homo sapiens, clone p4-176 from a chromosome 4 plasmid library;
            vector pBluescript II KS + (Stratagene)
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 486)
  AUTHORS   Goold,R.D., diSibio,G., Dugaiczyk,A., Smith,K.A., Xu,H.,
            Magrane,G., Lang,D.B., Cox,D.R., Masters,S.B. and Myers,R.M.
  TITLE     Sequence-Tagged Sites for Human Chromosome 4
  JOURNAL   Unpublished (1992) see COMMENT for author address
  STANDARD  full automatic
COMMENT     PCR components: 25 ng of human genomic DNA, 10 pmol of each
            oligonucleotide, 200 micro-M dNTPs, 0.25 U Taq  polymerase (Cetus)
            in 10 micro-l of 50 mM KCl-20 mM  Tris-HCl, pH 8.3 (at room temp),
            2.5 mM MgCl-2.  Initial denaturation at 94degC for 1.5 min, then 30
            cycles of  94degC for 15 sec, 62degC for 23 sec, and 72degC for 30
            sec, followed by a final extension at 72degC for 3.5 min, using a
            Perkin-Elmer 9600 thermocycler. PCR-amplified product size 186 bp.
            Sequence submitted by:
            Human Genome Mapping Center
            Box 0925
            University of California San Francisco
            San Francisco, CA 94143-0925  USA
            Phone: (415) 502-1612   Fax: (415) 476-8391
            e-mail: hgmcprobes@cgl.ucsf.edu
FEATURES             Location/Qualifiers
     misc_feature    70..92
                     /label=Primer1
     misc_feature    complement(233..255)
                     /label=Primer2
BASE COUNT      101 a    144 c     96 g    141 t      4 others
ORIGIN
        1 ggctcctngg atagtagtcc aattctgatg tctcctttat agtctctggg gggaattctg
       61 tactggaaaa tccacaggcc aggaaagata actgtggcct tattccactt gaccacccac
      121 agccatcctg tccttcctga ggcatccttt gnctaacgcc tgccaggccc ctgggntttg
      181 gtagaatcct cctctctatc gctgtcacca tgctgcttgc actgctgttc accagctcag
      241 gcacttacat cttcaagtgt ctctttaccc caccttgccc aaagaaggct gtgagtctcc
      301 cttggatggc agctgcatgt nctgcccggc ctaaatgccc ctactttcat gccttgcttc
      361 acaccctaca tgccatccac tttctaccgc aattggtgac tatttatcct agcaggtact
      421 aggtagcatc attcaatctt tgccatcctt aaagttgaaa catgagggag tgtacttact
      481 tgatcc
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human chromosome 4 sequence-tagged site STS4-21
Date: 21 Aug 92 10:07:45 GMT
Distribution: bionet
Checksum: 07535     3

LOCUS       HUM4STS21     320 bp ds-DNA             PRI       21-AUG-1992
DEFINITION  Human chromosome 4 sequence-tagged site STS4-21
ACCESSION   M95319
KEYWORDS    STS; human chromosome 4; sequence tagged site.
SOURCE      Homo sapiens, clone L4-12 from the C25 lambda library (Pritchard,
            Genomics 4, 408, 1989); vector - Lambda Dash II (Stratagene)
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 320)
  AUTHORS   Goold,R.D., diSibio,G., Dugaiczyk,A., Smith,K.A., Xu,H.,
            Magrane,G., Lang,D.B., Cox,D.R., Masters,S.B. and Myers,R.M.
  TITLE     Sequence-Tagged Sites for Human Chromosome 4
  JOURNAL   Unpublished (1992) see COMMENT for author address
  STANDARD  full automatic
COMMENT     PCR components: 25 ng of human genomic DNA, 40 pmol of each
            oligonucleotide, 200 micro-M dNTPs, 0.25 U Taq  polymerase (Cetus)
            in 10 micro-l of 50 mM KCl-20 mM  Tris-HCl, pH 8.3 (at room temp),
            2.5 mM MgCl-2.  Initial denaturation at 94degC for 1.5 min, then 30
            cycles of  94degC for 15 sec, 61degC for 23 sec, and 72degC for 30
            sec, followed by a final extension at 72degC for 3.5 min, using a
            Perkin-Elmer 9600 thermocycler. PCR-amplified product size 320 bp.
            Sequence submitted by:
            Human Genome Mapping Center
            Box 0925
            University of California San Francisco
            San Francisco, CA 94143-0925  USA
            Phone: (415) 502-1612   Fax: (415) 476-8391
            e-mail: hgmcprobes@cgl.ucsf.edu
FEATURES             Location/Qualifiers
     misc_feature    1..26
                     /label=Primer1
     misc_feature    complement(296..320)
                     /label=Primer2
BASE COUNT       74 a     59 c     30 g    157 t
ORIGIN
        1 cggatcattt atatgttatt tacttctttt atcttacttc ttatagaatc cttcttcttc
       61 cttactttga gcatttgatt atatgcactt gtagtagtct tatttggctc aaatctgttt
      121 tgtgttttct gaatgtcctt cagttgaata tttatctctt cttaacaaaa gcaaagtttt
      181 ctgttatatt tatttgaata acttctatcc ttctttgctc aactccttct tgaacactaa
      241 taattcttaa attttgactt ttttcttttt ctttcttttt tttaacctag ccattaacca
      301 cctaatatag atctgtcttt
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human chromosome 4 sequence-tagged site STS4-473
Date: 21 Aug 92 10:10:45 GMT
Distribution: bionet
Checksum: 24652     3

LOCUS       HUM4STS473    385 bp ds-DNA             PRI       21-AUG-1992
DEFINITION  Human chromosome 4 sequence-tagged site STS4-473
ACCESSION   L00880
KEYWORDS    STS; human chromosome 4; sequence tagged site.
SOURCE      Homo sapiens, clone p4-521 from a chromosome 4 plasmid library;
            vector pBluescript II KS + (Stratagene)
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 385)
  AUTHORS   Goold,R.D., diSibio,G., Dugaiczyk,A., Smith,K.A., Xu,H.,
            Magrane,G., Lang,D.B., Cox,D.R., Masters,S.B. and Myers,R.M.
  TITLE     Sequence-Tagged Sites for Human Chromosome 4
  JOURNAL   Unpublished (1992) see COMMENT for author address
  STANDARD  full automatic
COMMENT     PCR components: 25 ng of human genomic DNA, 10 pmol of each
            oligonucleotide, 200 micro-M dNTPs, 0.25 U Taq  polymerase (Cetus)
            in 10 micro-l of 50 mM KCl-20 mM  Tris-HCl, pH 8.3 (at room temp),
            2.5 mM MgCl-2.  Initial denaturation at 94degC for 1.5 min, then 30
            cycles of  94degC for 15 sec, 62degC for 23 sec, and 72degC for 30
            sec, followed by a final extension at 72degC for 3.5 min, using a
            Perkin-Elmer 9600 thermocycler. PCR-amplified product size 271 bp.
            Sequence submitted by:
            Human Genome Mapping Center
            Box 0925
            University of California San Francisco
            San Francisco, CA 94143-0925  USA
            Phone: (415) 502-1612   Fax: (415) 476-8391
            e-mail: hgmcprobes@cgl.ucsf.edu
FEATURES             Location/Qualifiers
     misc_feature    22..43
                     /label=Primer1
     misc_feature    complement(270..292)
                     /label=Primer2
BASE COUNT       97 a    103 c     79 g    103 t      3 others
ORIGIN
        1 gatcccaagt cccgctagcc ttgtctgccc tgtagtaagc caccctctgt tcatactgtg
       61 gcggatactg taagtgactc attcaacact gctcctattc cttttatccc tcaccttcct
      121 ctactacaga ggctagaaag ctaaatactt catatctcta atggccatgc agntggaaat
      181 gcccctgtaa cactgctctg gccaaagaaa cctaagcaga attctgctgg ctgtgtccca
      241 tcagncacct tcttcctgcc taagatgaaa ccaggtatct agtgttgtgg cagccatgag
      301 ggaagactgg aagagttgca gtcagtttgg cttgatatca tgctaaagac acaaacccag
      361 gaactgnctg cttctagaca tttgg
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Rattus norvegicus satellite repeat.
Date: 21 Aug 92 10:12:44 GMT
Distribution: bionet
Checksum: 53492     2

LOCUS       RATRSP        396 bp ds-DNA             ROD       21-AUG-1992
DEFINITION  Rattus norvegicus satellite repeat.
ACCESSION   M99615
KEYWORDS    satellite DNA.
SOURCE      Rattus norvegicus (strain LEW/N) liver DNA.
  ORGANISM  Rattus norvegicus
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Rodentia; Myomorpha; Muridae; Murinae.
REFERENCE   1  (bases 1 to 396)
  AUTHORS   Zha,H., Remmers,E.F., Goldmuntz,E.A., Cash,J.M., Crofford,L.J.,
            Mathern,P. and Wilder,R.L.
  TITLE     Simple sequence repeat identified in LEW/N rat genomic DNA
  JOURNAL   Unpublished (1992)
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     satellite       26..63
                     /rpt_type=TANDEM
BASE COUNT      102 a     77 c    108 g    109 t
ORIGIN
        1 gatcaaactt ttgcctaata gttacgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg
       61 tgtcgaggag tggttgttat tcacatgaaa tgtgcccttg tagtgtctat attctgagag
      121 ctcaccaagc ccgatgctta gacatatttt ctggataaat gagttaaata ctatggataa
      181 agtgtccacc cggtgcttgt ttgctagaga acttcctaga aacagtttca atgatgtgcc
      241 gtgacactca aggaaaagga gacaacgaga cacataaaga gaggcagttg gaggggcaga
      301 ccagagagcc aaggctaaga aggctgcccc tggcaggctg tctctgtcag ctctgggcct
      361 gagcccctca cttacagcag aatgctaatc ttgatc
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Synechococcus sp. photosystem I subunit IV (psaE) gene, complete
Date: 20 Aug 92 10:04:58 GMT
Distribution: bionet
Checksum: 64891     3

LOCUS       SYOPSAEFPG    701 bp ds-DNA             BCT       20-AUG-1992
DEFINITION  Synechococcus sp. photosystem I subunit IV (psaE) gene, complete
            cds; and formamidopyrimidine DNA N-glycosylase (fpg) gene, 5' end.
ACCESSION   M99432
KEYWORDS    DNA N-glycosylase; formamidopyrimidine-DNA glycosylase;
            photosystem I subunit IV.
SOURCE      Synechococcus sp. (library: PCC 6301) DNA.
  ORGANISM  Synechococcus sp.
            Prokaryotae; Gracilicutes; Oxyphotobacteria; Cyanobacteria;
            Chroococcales.
REFERENCE   1  (bases 1 to 701)
  AUTHORS   Rhiel,E. and Bryant,D.A.
  TITLE     Nucleotide sequence of the psaE gene of Synechococcus sp. PCC 6301
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     RBS             210..216
     CDS             225..452
                     /function="possible role in cyclic electron transport"
                     /standard_name="PsaE"
                     /product="photosystem I subunit IV"
                     /gene="psaE"
                     /evidence=EXPERIMENTAL
                     /codon_start=1
                     /translation="MAIARGDKVRILRPESYWFNEVGTVASVDQSGIKYPVVVRFEKV
                     NYNGFSGSDGGVNTNNFAEAELQVVAAAAKK"
     stem_loop       481..508
     CDS             561..701
                     /function="DNA N-glycosylase, removes formamidopyrimidine
                     (breakdown product from N7-alkylguanine, the major product
                     of DNA alkylation"
                     /product="formamidopyrimidine DNA N-glycosylase"
                     /gene="fpg"
                     /codon_start=1
                     /translation="LPELPEVETVRRGLTQQTLQRVCTGGEVLLSRTIATPTPELFLV
                     ALQ"
                     /partial
BASE COUNT      171 a    193 c    188 g    149 t
ORIGIN
        1 ctcgagatcc aaggacgctt caggcggagc agcaaggaca gtcataagcc aaggtcacgg
       61 aggtaggcgg acattatcgc actgggctgt agcgtccaga gaaacaaaac ttaaaggcga
      121 gaagcctcgg caattggaac accggtagta gtctagaaac gatgtctgca agctgattcc
      181 agccttttga gacatcgcag aagctcatcg aggaagcatc agttatggcc atcgcgcgtg
      241 gtgataaggt tcggatcctc cgcccagagt cctactggtt caacgaagtc ggcacggtgg
      301 cttcggtcga ccaaagcggc atcaagtatc ccgtggtcgt ccgcttcgag aaagtgaact
      361 acaacggctt cagcggctcg gatggcggtg tcaacaccaa caactttgcc gaagcagaat
      421 tacaagtggt tgcagcagcc gccaaaaaat agactgcttc tgtcttcaca agaggttcct
      481 gaaacggcta catcactgtg gccgtttttt gtggctgaat ttctcgccgt atactgcttc
      541 ggtcgcgatc gctctccact ttgcctgaac tgcccgaagt cgaaactgtc cgccgtggac
      601 tgactcaaca aaccctgcaa cgcgtttgca caggtggcga agtcttactc tctcgtacga
      661 tcgcgacacc cacgcccgaa ctgttcctcg tggcgctgca g
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human chromosome 4 sequence-tagged site STS4-95
Date: 21 Aug 92 10:11:23 GMT
Distribution: bionet
Checksum: 65484     3

LOCUS       HUM4STS95     420 bp ds-DNA             PRI       21-AUG-1992
DEFINITION  Human chromosome 4 sequence-tagged site STS4-95
ACCESSION   M95270
KEYWORDS    STS; human chromosome 4; sequence tagged site.
SOURCE      Homo sapiens, clone C4-155 from Los Alamos National Laboratory
            chromosome 4 cosmid library, plate 4-1-2R, pos 5A; vector SuperCos
            1 (Stratagene)
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 420)
  AUTHORS   Goold,R.D., diSibio,G., Dugaiczyk,A., Smith,K.A., Xu,H.,
            Magrane,G., Lang,D.B., Cox,D.R., Masters,S.B. and Myers,R.M.
  TITLE     Sequence-Tagged Sites for Human Chromosome 4
  JOURNAL   Unpublished (1992) see COMMENT for author address
  STANDARD  full automatic
COMMENT     PCR components: 25 ng of human genomic DNA, 10 pmol of each
            oligonucleotide, 200 micro-M dNTPs, 0.25 U Taq  polymerase (Cetus)
            in 10 micro-l of 50 mM KCl-20 mM  Tris-HCl, pH 8.3 (at room temp),
            2.5 mM MgCl-2.  Initial denaturation at 94degC for 1.5 min, then 30
            cycles of  94degC for 15 sec, 62degC for 23 sec, and 72degC for 30
            sec, followed by a final extension at 72degC for 3.5 min, using a
            Perkin-Elmer 9600 thermocycler. PCR-amplified product size 313 bp.
            Sequence submitted by:
            Human Genome Mapping Center
            Box 0925
            University of California San Francisco
            San Francisco, CA 94143-0925  USA
            Phone: (415) 502-1612   Fax: (415) 476-8391
            e-mail: hgmcprobes@cgl.ucsf.edu
FEATURES             Location/Qualifiers
     misc_feature    3..27
                     /label=Primer1
     misc_feature    complement(293..315)
                     /label=Primer2
BASE COUNT      126 a     96 c     80 g    109 t      9 others
ORIGIN
        1 tnaggaagtg atttcaggag aatcttttca aaaccaaagt tattaataac tgcttctgaa
       61 attataattt gagactttgt acaaaacttg atttgggctc cagaactcct aatgatggtt
      121 caacaaatca gatggacctt catgtggttc caggccaaaa gagggggaaa gtaccacagt
      181 tttctgtaaa cacatctaaa gttttcatgt ctccttcccc ttgttcctcc cagtcacctc
      241 ccttccctcc cttctactca actgtttaga gaaagcgtaa gaaatctggg actctcttag
      301 gcactaaggt gacagnagac aacacaggtc ctcgtctncc ctagaagacc tcttagtcta
      361 gnaaggaagg gaggagtaca caaagattcc ncaactaagn ggntagagga acacaccnnc
//