From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human glucokinase (GCK) mRNA, complete cds.
Date: 12 Sep 92 10:02:29 GMT
Distribution: bionet
Checksum: 52336     6

LOCUS       HUMGLCKNS    2731 bp ss-mRNA            PRI       12-SEP-1992
DEFINITION  Human glucokinase (GCK) mRNA, complete cds.
ACCESSION   M90299
KEYWORDS    D-hexose 6-phosphotransferase; glucokinase.
SOURCE      Homo sapiens (library: lambda gt11; lambda ZAP) adult pancreatic
            islets cDNA to mRNA.
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 2731)
  AUTHORS   Koranyi,L.I., Tanizawa,Y., Welling,C.M., Rabin,D.U. and
            Permutt,M.A.
  TITLE     Human islet glucokinase gene: Isolation and sequence analysis of a
            full length cDNA
  JOURNAL   Diabetes 41, 807-811 (1992)
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     mRNA            1..2731
                     /gene="GCK"
                     /evidence=EXPERIMENTAL
                     /citation=[1]
     5'UTR           1..458
                     /gene="GCK"
                     /note="putative"
                     /citation=[1]
     CDS             459..1856
                     /EC_number="2.7.1.1"
                     /standard_name="ATP:D-hexose 6-phosphotransferase"
                     /product="glucokinase"
                     /gene="GCK"
                     /note="putative"
                     /codon_start=1
                     /translation="MLDDRARMEAAKKEKVEQILAEFQLQEEDLKKVMRRMQKEMDRG
                     LRLETHEEASVKMLPTYVRSTPEGSEVGDFLSLDLGGTNFRVMLVKVGEGEEGQWSVK
                     TKHQMYSIPEDAMTGTAEMLFDYISECISDFLDKHQMKHKKLPLGFTFSFPVRHEDID
                     KGILLNWTKGFKASGAEGNNVVGLLRDAIKRRGDFEMDVVAMVNDTVATMISCYYEDH
                     QCEVGMIVGTGCNACYMEEMQNVELVEGDEGRMCVNTEWGAFGDSGELDEFLLEYDRL
                     VDESSANPGQQLYEKLIGGKYMGELVRLVLLRLVDENLLFHGEASEQLRTRGAFETRF
                     VSQVESDTGDRKQIYNILSTLGLRPSTTDCDIVRRACESVSTRAAHMCSAGLAGVINR
                     MRESRSEDVMRITVGVDGSVYKLHPSFKERFHASVRRLTPSCEITFIESEEGSGRGAA
                     LVSAVACKKACMLGQ"
                     /citation=[1]
     3'UTR           1857..2723
                     /gene="GCK"
                     /citation=[1]
     polyA_signal    2695..2701
                     /gene="GCK"
                     /citation=[1]
     polyA_site      2723
                     /gene="GCK"
                     /evidence=EXPERIMENTAL
BASE COUNT      626 a    778 c    864 g    463 t
ORIGIN
        1 ccgagcggcg cctgagcccc agggaagcag gctaggatgt gagagacaca gtcacctgca
       61 gcctaattac tcaaaagctg tccccaggtc acagaaggga gaggacattt cccactgaat
      121 ctgtctgaag gacactaagc cccacagctc aacacaacca ggagagaaag cgctgaggac
      181 gccacccaag cgcccagcaa tggccctgcc tggagaacat ccaggctcag tgaggaaggg
      241 tccagaaggg aatgcttgcc gactcgttgg agaacaatga aaaggaggaa actgtgactg
      301 aacctcaaac cccaaaccag cccgaggaga accacattct cccagggacc cagggcgggc
      361 cgtgacccct gcggcggaga agccttggat atttccactt cagaagccta ctggggaagg
      421 ctgaggggtc ccagctcccc acgctggctg ctgtgcagat gctggacgac agagccagga
      481 tggaggccgc caagaaggag aaggtagagc agatcctggc agagttccag ctgcaggagg
      541 aggacctgaa gaaggtgatg agacggatgc agaaggagat ggaccgcggc ctgaggctgg
      601 agacccatga agaggccagt gtgaagatgc tgcccaccta cgtgcgctcc accccagaag
      661 gctcagaagt cggggacttc ctctccctgg acctgggtgg cactaacttc agggtgatgc
      721 tggtgaaggt gggagaaggt gaggaggggc agtggagcgt gaagaccaaa caccagatgt
      781 actccatccc cgaggacgcc atgaccggca ctgctgagat gctcttcgac tacatctctg
      841 agtgcatctc cgacttcctg gacaagcatc agatgaaaca caagaagctg cccctgggct
      901 tcaccttctc ctttcctgtg aggcacgaag acatcgataa gggcatcctt ctcaactgga
      961 ccaagggctt caaggcctca ggagcagaag ggaacaatgt cgtggggctt ctgcgagacg
     1021 ctatcaaacg gagaggggac tttgaaatgg atgtggtggc aatggtgaat gacacggtgg
     1081 ccacgatgat ctcctgctac tacgaagacc atcagtgcga ggtcggcatg atcgtgggca
     1141 cgggctgcaa tgcctgctac atggaggaga tgcagaatgt ggagctggtg gagggggacg
     1201 agggccgcat gtgcgtcaat accgagtggg gcgccttcgg ggactccggc gagctggacg
     1261 agttcctgct ggagtatgac cgcctggtgg acgagagctc tgcaaacccc ggtcagcagc
     1321 tgtatgagaa gctcataggt ggcaagtaca tgggcgagct ggtgcggctt gtgctgctca
     1381 ggctcgtgga cgaaaacctg ctcttccacg gggaggcctc cgagcagctg cgcacacgcg
     1441 gagccttcga gacgcgcttc gtgtcgcagg tggagagcga cacgggcgac cgcaagcaga
     1501 tctacaacat cctgagcacg ctggggctgc gaccctcgac caccgactgc gacatcgtgc
     1561 gccgcgcctg cgagagcgtg tctacgcgcg ctgcgcacat gtgctcggcg gggctggcgg
     1621 gcgtcatcaa ccgcatgcgc gagagccgca gcgaggacgt aatgcgcatc actgtgggcg
     1681 tggatggctc cgtgtacaag ctgcacccca gcttcaagga gcggttccat gccagcgtgc
     1741 gcaggctgac gcccagctgc gagatcacct tcatcgagtc ggaggagggc agtggccggg
     1801 gcgcggccct ggtctcggcg gtggcctgta agaaggcctg tatgctgggc cagtgagagc
     1861 agtggccgca agcgcaggga ggatgccaca gccccacagc acccaggctc catggggaag
     1921 tgctccccac acgtgctcgc agcctggcgg ggcaggaggc ctggccttgt caggacccag
     1981 gccgcctgcc ataccgctgg ggaacagagc gggcctcttc cctcagtttt tcggtgggac
     2041 agccccaggg ccctaacggg ggtgcggcag gagcaggaac agagactctg gaagcccccc
     2101 acctttctcg ctggaatcaa tttcccagaa gggagttgct cactcaggac tttgatgcat
     2161 ttccacactg tcagagctgt tggcctcgcc tgggcccagg ctctgggaag gggtgccctc
     2221 tggatcctgc tgtggcctca cttccctggg aactcatcct gtgtggggag gcagctccaa
     2281 cagcttgacc agacctagac ctgggccaaa agggcaggcc aggggctgct catcacccag
     2341 tcctggccat tttcttgcct gaggctcaag aggcccaggg agcaatggga gggggctcca
     2401 tggaggaggt gtcccaagct ttgaataccc cccagagacc ttttctctcc cataccatca
     2461 ctgagtggct tgtgattctg ggatggaccc tcgcagcagg tgcaagagac agagccccca
     2521 agcctctgcc ccaaggggcc cacaaagggg agaagggcca gccctacatc ttcagctccc
     2581 atagcgctgg ctcaggaaga aaccccaagc agcattcagc acaccccaag ggacaacccc
     2641 atcatatgac atgccaccct ctccatgccc aacctaagat tgtgtgggtt ttttaattaa
     2701 aaatgttaaa agttttaaac atgaaaaaaa a
//