From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Synechococcus sp. putative ORF1, 5' end; ORF2, complete cds; psaC
Date: 9 Apr 92 10:05:27 GMT
Distribution: bionet
Checksum: 27344     5

LOCUS       SYOPSACA     1800 bp ds-DNA             BCT       09-APR-1992
DEFINITION  Synechococcus sp. putative ORF1, 5' end; ORF2, complete cds; psaC
            gene, complete cds.
ACCESSION   M86238
KEYWORDS    photosystem I reaction center.
SOURCE      Synechococcus sp. (strain PR6000) (library: PCC 7002) DNA.
  ORGANISM  Synechococcus sp.
            Prokaryota; Bacteria; Gracilicutes; Oxyphotobacteria;
            Cyanobacteria; Chroococcales.
REFERENCE   1  (bases 1 to 1800)
  AUTHORS   Rhiel,E., Stirewalt,V.L., Gasparich,G.E. and Bryant,D.A.
  TITLE     The psaC genes of Synechococcus sp. PCC7002 and Cyanophora
            paradoxa: cloning and sequence analysis
  JOURNAL   Gene 112, 123-128 (1992)
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     CDS             complement(1..>44)
                     /note="putative ORF1"
                     /codon_start=1
                     /translation="MKGFKLDVTGRLQP"
     CDS             complement(53..331)
                     /note="open reading frame"
                     /codon_start=1
                     /translation="MAIRFQALEVVALSVPEAPRPIQEYLQDIDCLVGAIADPERTEK
                     IAPDQYQLKMRPIGFLDLYKFQPIVTLKIWCGRTTKSISKVWTTSCGV"
     RBS             complement(344..348)
     -10_signal      521..526
     misc_feature    535
                     /note="mRNA 5' endpoint determined by primer extension"
                     /evidence=EXPERIMENTAL
                     /partial
     RBS             571..575
     CDS             586..831
                     /function="terminal electron acceptor for photosystem I
                     reaction center"
                     /note="product binds the terminal electron accepting Fe-S
                     centers of photosystem I'"
                     /gene="psaC"
                     /evidence=EXPERIMENTAL
                     /codon_start=1
                     /translation="MSHSVKIYDTCIGCTQCVRACPLDVLEMVPWDGCKAGQIASSPR
                     TEDCVGCKRCETACPTDFLSIRVYLGAETTRSMGLAY"
     misc_feature    complement(1068..1672)
                     /note="putative cds"
BASE COUNT      485 a    396 c    388 g    531 t
ORIGIN
        1 atgggttgta accgaccagt cacatcgagt ttaaagccct tcataaaggg ttctagaccc
       61 cgcaactggt agtccaaact tttgatatgg acttggtagt gcgaccgcac caaattttca
      121 aggtcacaat gggctgaaat ttgtacagat ctaaaaagcc aatgggccgc attttgagtt
      181 ggtattgatc cggggcaatt ttttcggtgc gttctggatc ggcgatcgcc cccaccagac
      241 aatcaatgtc ttggagatac tcttgaatcg gacggggtgc ctctggtacg gagagggcaa
      301 caacttcgag agcttggaag cggatggcca tagtaaagtt ttgttcctaa aattttgtag
      361 tcaacatcga atcaatagaa accctggttt tgcccccaaa aagcacccca gaaaatttca
      421 aaaaacgctc aaaattgcct agagacgccg gttttttagg gatatgtaac gcaacgtaaa
      481 cttatatcac cttttcgcca agggacttct agccctgcgg taaactaacc cataatccag
      541 aatgctgaca taatcaaacc acattcttgt aggagttaac actcaatgtc tcatagcgtt
      601 aaaatttacg acacttgcat tggctgcacc cagtgcgtcc gtgcctgtcc ccttgatgtc
      661 ctagagatgg ttccttggga tggctgcaaa gcgggtcaga tcgcatcttc tcctagaaca
      721 gaagattgcg ttggttgtaa gcggtgtgaa actgcttgcc ccaccgactt tctcagtatc
      781 cgggtttacc tcggtgccga gacaactcgt agtatgggtc tggcctacta aattcgccca
      841 tttaacacaa gcctttttgt attgggttat attaaaacac tatcccatta acgttttcaa
      901 ccattctggt catttccgga gaggcatttt agtcctctct tttttttatt gtccgctaca
      961 ttatatttgt ttcgccatta gaatgcggcg atccgcgtgg gtggcgatcg ccatgggtca
     1021 gacttgttca ggattaagtc ctatttttca aaacaatcaa gagtaaatta accaatccag
     1081 ttaatttctg tgggatcagt ctccttggcc gtgtttaggg tctctttaga gagtgggggt
     1141 tgagtcgctt tcgcgttggg ctgctgggcg agagatgttt tgggatgatg ggtggtctgc
     1201 caaaatcggc gatcgccaac atgttgattt aggatacgac gcaggaaaaa attcaaggtt
     1261 ttctggggaa tataaccttt caacaccgct aaactgccga ataacatatg gtttagccgt
     1321 tgctcatcaa gaattagagc gagctgctca gtggtcagta aatccgcttc aagtaaataa
     1381 tcaccaagcc gtttgctatc aattaagatc aactcttgtt tagcatactt tgcaaaaaaa
     1441 tcagccgtgg gttgacgaat ccagccccgc aaagtcaaaa tttcaccaaa ttttaaatca
     1501 ctataaattt gctgatcaaa aagagccgtt tgtaattggg cttcagaaat taagcctgca
     1561 tcttcaagga cttcgccgat caactgaatt ttcggaccat tgttggtaga tgctgcattc
     1621 ataagcgcta tttcaaccta ctgtgacgat gcaagaacaa tactttacag acccagtgat
     1681 actcattgcc gttaaaggat tgctctttta ttttttattt tccccataca aatataacgt
     1741 gacatctatt gttttaattg ggacgctaca gtacctattt tgttcaatct taatgcaatc
//