From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: E.coli pntA and pntB genes for pyridine nucleotide transhydrogenase
Date: 21 Jul 92 10:04:20 GMT
Article-I.D.: genbank.Jul.21.03.04.19.1992.18036
Distribution: bionet
Checksum: 04864     8

LOCUS       ECOPNT       3524 bp ds-DNA             BCT       21-JUL-1992
DEFINITION  E.coli pntA and pntB genes for pyridine nucleotide transhydrogenase
ACCESSION   X66086
KEYWORDS    proton pump; pyridine nucleotide transhydrogenase;
            pyridine nucleotide transhydrogenase alpha subunit;
            pyridine nucleotide transhydrogenase beta subunit;
            transmembrane protein.
SOURCE      Escherichia coli DNA.
  ORGANISM  Escherichia coli
            Prokaryotae; Gram-negative facultatively anaerobic rods;
            Enterobacteriaceae.
REFERENCE   1  (bases 1 to 3524)
  AUTHORS   Ahmad,S., Glavas,N.A. and Bragg,P.D.
  TITLE     A mutation at glycine-314 of the beta subunit of the E. coli
            pyridine nucleotide transhydrogenase abolishes activity and affects
            NADP(H) induced a conformation of change
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
COMMENT     *source: strain=K12;
            See also X04195
            From EMBL    entry ECPNT;  dated 10-JUN-1992.
FEATURES             Location/Qualifiers
     CDS             527..2059
                     /EC_number="1.6.1.1"
                     /note="alphasubunit"
                     /product="pyridine nucleotidetranshydrogenase"
                     /gene="pntA"
                     /codon_start=1
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCTCSPWRKYALMALAIILFGWMASVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     CDS             2070..3458
                     /EC_number="1.6.1.1"
                     /note="beta subunit"
                     /product="pyridine nucleotidetranshydrogenase"
                     /gene="pntB"
                     /codon_start=1
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
BASE COUNT      834 a    807 c    977 g    906 t
ORIGIN
        1 cataaaaata atccttcgcc ttgcgcaaac caggtactgg tattgttatt aacgagaaac
       61 gtggctgatt attgcattta aacggtgtaa ctgtctgcgt catttttcat atcacattcc
      121 ttaagccaat tttaatcctg ctcaaatgac cgtctatgct taaaaaacag ccgtatcagc
      181 atcattacta ctgaagcaac tgaattgtat aagttaattt aatgttaagt agtgattcgt
      241 gccggggcga tgtctcgttt tacccgaccg tcgaagacaa ttatcagtct ttatccggcg
      301 ttctaaggtg tttatcccac tatcacggct gaatcgttaa tattttgcga gttcacgccg
      361 aaatactgat ttttggcgct agatcacagg cataattttc agtacgttat agggcgtttg
      421 ttactaattt attttaacgg agtaacattt agctcgtaca tgagcagctt gtgtggctcc
      481 tgacacaggc aaaccatcat caataaaacc gatggaaggg aatatcatgc gaattggcat
      541 accaagagaa cggttaacca atgaaacccg tgttgcagca acgccaaaaa cagtggaaca
      601 gctgctgaaa ctgggtttta ccgtcgcggt agagagcggc gcgggtcaac tggcaagttt
      661 tgacgataaa gcgtttgtgc aagcgggcgc tgaaattgta gaagggaata gcgtctggca
      721 gtcagagatc attctgaagg tcaatgcgcc gttagatgat gaaattgcgt tactgaatcc
      781 tgggacaacg ctggtgagtt ttatctggcc tgcgcagaat ccggaattaa tgcaaaaact
      841 tgcggaacgt aacgtgaccg tgatggcgat ggactctgtg ccgcgtatct cacgcgcaca
      901 atcgctggac gcactaagct cgatggcgaa catcgccggt tatcgcgcca ttgttgaagc
      961 ggcacatgaa tttgggcgct tctttaccgg gcaaattact gcggccggga aagtgccacc
     1021 ggcaaaagtg atggtgattg gtgcgggtgt tgcaggtctg gccgccattg gcgcagcaaa
     1081 cagtctcggc gcgattgtgc gtgcattcga cacccgcccg gaagtgaaag aacaagttca
     1141 aagtatgggc gcggaattcc tcgagctgga ttttaaagag gaagctggca gcggcgatgg
     1201 ctatgccaaa gtgatgtcgg acgcgttcat caaagcggaa atggaactct ttgccgccca
     1261 ggcaaaagag gtcgatatca ttgtcaccac cgcgcttatt ccaggcaaac cagcgccgaa
     1321 gctaattacc cgtgaaatgg ttgactccat gaaggcgggc agtgtgattg tcgacctggc
     1381 agcccaaaac ggcggcaact gtgaatacac cgtgccgggt gaaatcttca ctacggaaaa
     1441 tggtgtcaaa gtgattggtt ataccgatct tccgggccgt ctgccgacgc aatcctcaca
     1501 gctttacggc acaaacctcg ttaatctgct gaaactgttg tgcaaagaga aagacggcaa
     1561 tatcactgtt gattttgatg atgtggtgat tcgcggcgtg accgtgatcc gtgcgggcga
     1621 aattacctgg ccggcaccgc cgattcaggt atcagctcag ccgcaggcgg cacaaaaagc
     1681 ggcaccggaa gtgaaaactg aggaaaaatg tacctgctca ccgtggcgta aatacgcgtt
     1741 gatggcgctg gcaatcattc tttttggctg gatggcaagc gttgcgccga aagaattcct
     1801 tgggcacttc accgttttcg cgctggcctg cgttgtcggt tattacgtgg tgtggaatgt
     1861 atcgcacgcg ctgcatacac cgttgatgtc ggtcaccaac gcgatttcag ggattattgt
     1921 tgtcggagca ctgttgcaga ttggccaggg cggctgggtt agcttcctta gttttatcgc
     1981 ggtgcttata gccagcatta atattttcgg tggcttcacc gtgactcagc gcatgctgaa
     2041 aatgttccgc aaaaattaag gggtaacata tgtctggagg attagttaca gctgcataca
     2101 ttgttgccgc gatcctgttt atcttcagtc tggccggtct ttcgaaacat gaaacgtctc
     2161 gccagggtaa caacttcggt atcgccggga tggcgattgc gttaatcgca accatttttg
     2221 gaccggatac gggtaatgtt ggctggatct tgctggcgat ggtcattggt ggggcaattg
     2281 gtatccgtct ggcgaagaaa gttgaaatga ccgaaatgcc agaactggtg gcgatcctgc
     2341 atagcttcgt gggtctggcg gcagtgctgg ttggctttaa cagctatctg catcatgacg
     2401 cgggaatggc accgattctg gtcaatattc acctgacgga agtgttcctc ggtatcttca
     2461 tcggggcggt aacgttcacg ggttcggtgg tggcgttcgg caaactgtgt ggcaagattt
     2521 cgtctaaacc attgatgctg ccaaaccgtc acaaaatgaa cctggcggct ctggtcgttt
     2581 ccttcctgct gctgattgta tttgttcgca cggacagcgt cggcctgcaa gtgctggcat
     2641 tgctgataat gaccgcaatt gcgctggtat tcggctggca tttagtcgcc tccatcggtg
     2701 gtgcagatat gccagtggtg gtgtcgatgc tgaactcgta ctccggctgg gcggctgcgg
     2761 ctgcgggctt tatgctcagc aacgacctgc tgattgtgac cggtgcgctg gtcggttctt
     2821 cgggggctat cctttcttac attatgtgta aggcgatgaa ccgttccttt atcagcgtta
     2881 ttgcgggtgg tttcggcacc gacggctctt ctactggcga tgatcaggaa gtgggtgagc
     2941 accgcgaaat caccgcagaa gagacagcgg aactgctgaa aaactcccat tcagtgatca
     3001 ttactccggg gtacggcatg gcagtcgcgc aggcgcaata tcctgtcgct gaaattactg
     3061 agaaattgcg cgctcgtggt attaatgtgc gtttcggtat ccacccggtc gcggggcgtt
     3121 tgcctggaca tatgaacgta ttgctggctg aagcaaaagt accgtatgac atcgtgctgg
     3181 aaatggacga gatcaatgat gactttgctg ataccgatac cgtactggtg attggtgcta
     3241 acgatacggt taacccggcg gcgcaggatg atccgaagag tccgattgct ggtatgcctg
     3301 tgctggaagt gtggaaagcg cagaacgtga ttgtctttaa acgttcgatg aacactggct
     3361 atgctggtgt gcaaaacccg ctgttcttca aggaaaacac ccacatgctg tttggtgacg
     3421 ccaaagccag cgtggatgca atcctgaaag ctctgtaacc ctcgactctg ctgaggccgt
     3481 cactctttat tgagatcgct taacagaacg gcgatgcgac tcta
//