HDTenEightyP/NetNews huggingface dataset:
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human chromosome 4 sequence-tagged site STS4-288
Date: 21 Aug 92 10:08:36 GMT
Distribution: bionet
Checksum: 31515     3

LOCUS       HUM4STS288    477 bp ds-DNA             PRI       21-AUG-1992
DEFINITION  Human chromosome 4 sequence-tagged site STS4-288
ACCESSION   L00779
KEYWORDS    STS; human chromosome 4; sequence tagged site.
SOURCE      Homo sapiens, clone p4-205 from a chromosome 4 plasmid library;
            vector pBluescript II KS + (Stratagene)
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 477)
  AUTHORS   Goold,R.D., diSibio,G., Dugaiczyk,A., Smith,K.A., Xu,H.,
            Magrane,G., Lang,D.B., Cox,D.R., Masters,S.B. and Myers,R.M.
  TITLE     Sequence-Tagged Sites for Human Chromosome 4
  JOURNAL   Unpublished (1992) see COMMENT for author address
  STANDARD  full automatic
COMMENT     PCR components: 25 ng of human genomic DNA, 10 pmol of each
            oligonucleotide, 200 micro-M dNTPs, 0.25 U Taq  polymerase (Cetus)
            in 10 micro-l of 50 mM KCl-20 mM  Tris-HCl, pH 8.3 (at room temp),
            2.5 mM MgCl-2.  Initial denaturation at 94degC for 1.5 min, then 30
            cycles of  94degC for 15 sec, 62degC for 23 sec, and 72degC for 30
            sec, followed by a final extension at 72degC for 3.5 min, using a
            Perkin-Elmer 9600 thermocycler. PCR-amplified product size 221 bp.
            Sequence submitted by:
            Human Genome Mapping Center
            Box 0925
            University of California San Francisco
            San Francisco, CA 94143-0925  USA
            Phone: (415) 502-1612   Fax: (415) 476-8391
            e-mail: hgmcprobes@cgl.ucsf.edu
FEATURES             Location/Qualifiers
     misc_feature    84..106
                     /label=Primer1
     misc_feature    complement(283..304)
                     /label=Primer2
BASE COUNT      165 a     93 c    111 g    104 t      4 others
ORIGIN
        1 gcctcacctg aggtcaggag tncaagacca gcctgaccaa catgaaacgc cctctctact
       61 aaaaatacaa aaaattagcc agccatggtg gcatgcaccc gtaattctag ctacttggga
      121 ggctgaagca ggaaaattgc tgagcccagg aagcaggggt tgcagtgagc cgagattgca
      181 ccattgcact ccagcctggg caacaagaat gaaactccat ctcaaagaaa aaaaaaataa
      241 taatatgcat gagtatgaag ggaaaaatta acgacttngt acatctgcca agccagctga
      301 ggagtttgaa aatgtatttg ggatttaccc taagagtata taatgaatat cctgaaatat
      361 ctgtggatga tactgatatt gaactatggt tagacagcag ntgccagagc agggaaggtg
      421 atagagacag agaaaatttt aaaagncttc ccctctatgc ccatagttga tagaagg
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human chromosome 4 sequence-tagged site STS4-247
Date: 21 Aug 92 10:08:09 GMT
Distribution: bionet
Checksum: 17872     3

LOCUS       HUM4STS247    450 bp ds-DNA             PRI       21-AUG-1992
DEFINITION  Human chromosome 4 sequence-tagged site STS4-247
ACCESSION   L00761
KEYWORDS    STS; human chromosome 4; sequence tagged site.
SOURCE      Homo sapiens, clone p4-190 from a chromosome 4 plasmid library;
            vector pBluescript II KS + (Stratagene)
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 450)
  AUTHORS   Goold,R.D., diSibio,G., Dugaiczyk,A., Smith,K.A., Xu,H.,
            Magrane,G., Lang,D.B., Cox,D.R., Masters,S.B. and Myers,R.M.
  TITLE     Sequence-Tagged Sites for Human Chromosome 4
  JOURNAL   Unpublished (1992) see COMMENT for author address
  STANDARD  full automatic
COMMENT     PCR components: 25 ng of human genomic DNA, 10 pmol of each
            oligonucleotide, 200 micro-M dNTPs, 0.25 U Taq  polymerase (Cetus)
            in 10 micro-l of 50 mM KCl-20 mM  Tris-HCl, pH 8.3 (at room temp),
            2.5 mM MgCl-2.  Initial denaturation at 94degC for 1.5 min, then 30
            cycles of  94degC for 15 sec, 62degC for 23 sec, and 72degC for 30
            sec, followed by a final extension at 72degC for 3.5 min, using a
            Perkin-Elmer 9600 thermocycler. PCR-amplified product size 191 bp.
            Sequence submitted by:
            Human Genome Mapping Center
            Box 0925
            University of California San Francisco
            San Francisco, CA 94143-0925  USA
            Phone: (415) 502-1612   Fax: (415) 476-8391
            e-mail: hgmcprobes@cgl.ucsf.edu
FEATURES             Location/Qualifiers
     misc_feature    78..101
                     /label=Primer1
     misc_feature    complement(243..268)
                     /label=Primer2
BASE COUNT      151 a     80 c     81 g    132 t      6 others
ORIGIN
        1 gatcagcttt gtttattccc ccacgctatt tatgacaatt ttactttttn gncattatat
       61 atttaaataa aatgtatata agccgatgat acatgagtac caaaaggtta aaaaaaaaca
      121 ttgtttcagt acaagctagt tttagcatct tggaaacatc tagtaaaggt taagttacca
      181 aatttgaatt ggatgatggt gagggcaaat acaaaacaaa agtagttaat tatcataaaa
      241 acctaaaagc attctgtttt cagattgctt tatgtcttaa aatttnngnt cccttgtaaa
      301 gaaaccaaaa ctagaattca ccaaggattt attgtgggcc agaagacaca tgtggacaac
      361 cactaataga tccactagtt ctagagcggc cgccacgcgg gggggctcca attcgcccta
      421 tagtgagtcg gnttacaatt cactggccgc
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human polymorphic microsatellite DNA.
Date: 19 Aug 92 10:01:03 GMT
Distribution: bionet
Checksum: 20889     2

LOCUS       HUM9PLYSAT    352 bp ds-DNA             PRI       19-AUG-1992
DEFINITION  Human polymorphic microsatellite DNA.
ACCESSION   M99149
KEYWORDS    microsatellite polymorphism.
SOURCE      Homo sapiens DNA.
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 352)
  AUTHORS   Bowcock,A.M.Ph.D.., Osborne-Lawrence,S.L., Barnes,R.I.,
            Chakravarti,A., Washington,S. and Dunn,C.
  TITLE     A linkage map of human chromosome 13q containing microsatellite
            polymorphisms
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     satellite       1..352
                     /note="map=13q; awaiting approval /map qualifier"
BASE COUNT       93 a     62 c     54 g    135 t      8 others
ORIGIN
        1 nggaattgca atgaagaaca caatataaat ttatcttttt aacaatttta agtgttcagt
       61 attgttaact ataaacacat tgttgtaaaa cagatctcta gaacttcttc atcttgcatg
      121 actgaaactc tacacccatt gaacaacttc ccttttgcct ctctgtttcc taaaagacct
      181 ctccctctgg cccctggaaa ccaccactct actttactat atgtgtgtgt gtgtgtgtgt
      241 gtgtgtgtgt gtgtgtgtgt gtataatatt tattccagtc taggttcaca atattttttn
      301 nntcttnnta aaaatgttnn tattttgtta tctttattta atagagacag gg
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Mouse T cell receptor rearranged beta-chain variable region,
Date: 15 Aug 92 10:02:46 GMT
Distribution: bionet
Checksum: 53469     2

LOCUS       MUSTCRB7H     213 bp ss-mRNA            ROD       15-AUG-1992
DEFINITION  Mouse T cell receptor rearranged beta-chain variable region,
            N_region, joining region, and constant region mRNA.
ACCESSION   M98565
KEYWORDS    T-cell receptor; beta-subunit receptor; constant region;
            joining region; variable region.
SOURCE      Mus musculus (strain BALB/cKh) adult spleen cDNA to mRNA.
  ORGANISM  Mus musculus
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Rodentia; Myomorpha; Muridae; Murinae.
REFERENCE   1  (bases 1 to 213)
  AUTHORS   Solheim,J.C., Martinko,J.M., Alexander-Miller,M. and Connolly,J.M.
  TITLE     Biased T Cell Receptor Usage by Ld-restricted Tum-Peptide-specific
            Cytotoxic T Lymphocyte Clones
  JOURNAL   Unpublished (1992)
  STANDARD  full staff_entry
BASE COUNT       55 a     60 c     47 g     51 t
ORIGIN      Chromosome 6
        1 ggatacaagg cctccagacc aagccaagag aacttctccc tcattctgga gttggctacc
       61 ccctctcaga catcagtgta cttctgtgcc agctttagtc aaaacacctt gtactttggt
      121 gcgggcaccc gactatcggt gctagaggat ctgagaaatg tgactccacc caaggtctcc
      181 ttgtttgagc catcaaaagc agagattgca aac
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human chromosome 4 sequence-tagged site STS4-333
Date: 21 Aug 92 10:09:03 GMT
Distribution: bionet
Checksum: 48171     3

LOCUS       HUM4STS333    370 bp ds-DNA             PRI       21-AUG-1992
DEFINITION  Human chromosome 4 sequence-tagged site STS4-333
ACCESSION   L00913
KEYWORDS    STS; human chromosome 4; sequence tagged site.
SOURCE      Homo sapiens, clone C4-113 from Los Alamos National Laboratory
            chromosome 4 cosmid library, 1E11; vector SuperCos 1 (Stratagene)
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 370)
  AUTHORS   Goold,R.D., diSibio,G., Dugaiczyk,A., Smith,K.A., Xu,H.,
            Magrane,G., Lang,D.B., Cox,D.R., Masters,S.B. and Myers,R.M.
  TITLE     Sequence-Tagged Sites for Human Chromosome 4
  JOURNAL   Unpublished (1992) see COMMENT for author address
  STANDARD  full automatic
COMMENT     PCR components: 25 ng of human genomic DNA, 10 pmol of each
            oligonucleotide, 200 micro-M dNTPs, 0.25 U Taq  polymerase (Cetus)
            in 10 micro-l of 50 mM KCl-20 mM  Tris-HCl, pH 8.3 (at room temp),
            2.5 mM MgCl-2.  Initial denaturation at 94degC for 1.5 min, then 30
            cycles of  94degC for 15 sec, 62degC for 23 sec, and 72degC for 30
            sec, followed by a final extension at 72degC for 3.5 min, using a
            Perkin-Elmer 9600 thermocycler. PCR-amplified product size 181 bp.
            Sequence submitted by:
            Human Genome Mapping Center
            Box 0925
            University of California San Francisco
            San Francisco, CA 94143-0925  USA
            Phone: (415) 502-1612   Fax: (415) 476-8391
            e-mail: hgmcprobes@cgl.ucsf.edu
FEATURES             Location/Qualifiers
     misc_feature    43..67
                     /label=Primer1
     misc_feature    complement(199..223)
                     /label=Primer2
BASE COUNT      141 a     61 c     56 g    101 t     11 others
ORIGIN
        1 atnctcctgc aattcctcca tgnttatgca tacaaattta tttagcatgc ataagcatga
       61 gggaataaca aaagtgaaac agtttgcaaa attttcagaa agcanatcac catacttttt
      121 agcattgctt gtaaacaaca gagaagagat aactgtaggt ttanctctgt agagttagaa
      181 gacttgtgct gaacgaagct tccacctaga gattcagaaa actanttnng nattaanatc
      241 cataaaaagc taattttgna acataaaata gtcaagatca cattcattgg aaaaattgta
      301 tatgaaaaat gagactatgg attgaancat ctttacatac aatgaaacca tacccaaaga
      361 cttgtccaca
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human chromosome 4 sequence-tagged site STS4-244
Date: 21 Aug 92 10:08:06 GMT
Distribution: bionet
Checksum: 47988     3

LOCUS       HUM4STS244    469 bp ds-DNA             PRI       21-AUG-1992
DEFINITION  Human chromosome 4 sequence-tagged site STS4-244
ACCESSION   L00758
KEYWORDS    STS; human chromosome 4; sequence tagged site.
SOURCE      Homo sapiens, clone p4-186 from a chromosome 4 plasmid library;
            vector pBluescript II KS + (Stratagene)
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 469)
  AUTHORS   Goold,R.D., diSibio,G., Dugaiczyk,A., Smith,K.A., Xu,H.,
            Magrane,G., Lang,D.B., Cox,D.R., Masters,S.B. and Myers,R.M.
  TITLE     Sequence-Tagged Sites for Human Chromosome 4
  JOURNAL   Unpublished (1992) see COMMENT for author address
  STANDARD  full automatic
COMMENT     PCR components: 25 ng of human genomic DNA, 10 pmol of each
            oligonucleotide, 200 micro-M dNTPs, 0.25 U Taq  polymerase (Cetus)
            in 10 micro-l of 50 mM KCl-20 mM  Tris-HCl, pH 8.3 (at room temp),
            2.5 mM MgCl-2.  Initial denaturation at 94degC for 1.5 min, then 30
            cycles of  94degC for 15 sec, 62degC for 23 sec, and 72degC for 30
            sec, followed by a final extension at 72degC for 3.5 min, using a
            Perkin-Elmer 9600 thermocycler. PCR-amplified product size 186 bp.
            Sequence submitted by:
            Human Genome Mapping Center
            Box 0925
            University of California San Francisco
            San Francisco, CA 94143-0925  USA
            Phone: (415) 502-1612   Fax: (415) 476-8391
            e-mail: hgmcprobes@cgl.ucsf.edu
FEATURES             Location/Qualifiers
     misc_feature    20..42
                     /label=Primer1
     misc_feature    complement(182..205)
                     /label=Primer2
BASE COUNT      132 a     90 c     86 g    154 t      7 others
ORIGIN
        1 ttcntgcagc ccgggggtat ctcagctcaa atgccatctc tcagaaaggt cttttttagc
       61 catacgtata gtcactctgt atcccaaagt agtactttat ttcctttata tcatcggtac
      121 tgcctaatag aaatgttctt atcgacttac ttgcatgctt attttctgtt tcctttcatt
      181 agaatgtacg aaccatgtct gtctttttct ccactgtatt cccagactct ggcagagtac
      241 ttgtcccata gtagctattc agtaaatgtn tgncgaatga tataaatgaa tgaatgaatg
      301 aggaggaagc taanaatctt ttaaaatcac gggtccagaa taatccctaa atacctccca
      361 atgtaatatt ctgtatttgn ggggagggtt caatgatgtg ttctagcttg taaatattaa
      421 aagtgatttn nataacctca gagcaaaagt catcctgagg aggcagaag
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human chromosome 4 sequence-tagged site STS4-383
Date: 21 Aug 92 10:09:41 GMT
Distribution: bionet
Checksum: 10653     3

LOCUS       HUM4STS383    431 bp ds-DNA             PRI       21-AUG-1992
DEFINITION  Human chromosome 4 sequence-tagged site STS4-383
ACCESSION   L00829
KEYWORDS    STS; human chromosome 4; sequence tagged site.
SOURCE      Homo sapiens, clone p4-328 from a chromosome 4 plasmid library;
            vector pBluescript II KS + (Stratagene)
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 431)
  AUTHORS   Goold,R.D., diSibio,G., Dugaiczyk,A., Smith,K.A., Xu,H.,
            Magrane,G., Lang,D.B., Cox,D.R., Masters,S.B. and Myers,R.M.
  TITLE     Sequence-Tagged Sites for Human Chromosome 4
  JOURNAL   Unpublished (1992) see COMMENT for author address
  STANDARD  full automatic
COMMENT     PCR components: 25 ng of human genomic DNA, 10 pmol of each
            oligonucleotide, 200 micro-M dNTPs, 0.25 U Taq  polymerase (Cetus)
            in 10 micro-l of 50 mM KCl-20 mM  Tris-HCl, pH 8.3 (at room temp),
            2.5 mM MgCl-2.  Initial denaturation at 94degC for 1.5 min, then 30
            cycles of  94degC for 15 sec, 62degC for 23 sec, and 72degC for 30
            sec, followed by a final extension at 72degC for 3.5 min, using a
            Perkin-Elmer 9600 thermocycler. PCR-amplified product size 165 bp.
            Sequence submitted by:
            Human Genome Mapping Center
            Box 0925
            University of California San Francisco
            San Francisco, CA 94143-0925  USA
            Phone: (415) 502-1612   Fax: (415) 476-8391
            e-mail: hgmcprobes@cgl.ucsf.edu
FEATURES             Location/Qualifiers
     misc_feature    30..53
                     /label=Primer1
     misc_feature    complement(171..194)
                     /label=Primer2
BASE COUNT      135 a     77 c     89 g    128 t      2 others
ORIGIN
        1 gatctccaga aaaaaactct gccctgaaaa catcttggtt ttaagccagt gagaactatt
       61 tcaggcatct gaccaccaga agagcaaaac aataaatttg tgttgtttta ggccactatg
      121 tttgtagtaa tttgttacag cagtgtgata gttaatttta agagtcaact tggctagact
      181 tgcatttggc aaatcactac tgtagatgtt gctgtaaagg tattttttag atgttataac
      241 atttacagtc agttgacctt aagtaanggg gattagactt cataatatgg gcaggcctta
      301 agagcagaga tggaggtttc ctaagggaga agaaattctg cctccangct gcaacatgga
      361 aaggcttgag ttttaccagc cttcaaattc aagacttgaa aacaacaact cttacctgaa
      421 tctccaggct c
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human chromosome 4 sequence-tagged site STS4-408
Date: 21 Aug 92 10:10:01 GMT
Distribution: bionet
Checksum: 13008     3

LOCUS       HUM4STS408    468 bp ds-DNA             PRI       21-AUG-1992
DEFINITION  Human chromosome 4 sequence-tagged site STS4-408
ACCESSION   L00843
KEYWORDS    STS; human chromosome 4; sequence tagged site.
SOURCE      Homo sapiens, clone p4-422 from a chromosome 4 plasmid library;
            vector pBluescript II KS + (Stratagene)
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 468)
  AUTHORS   Goold,R.D., diSibio,G., Dugaiczyk,A., Smith,K.A., Xu,H.,
            Magrane,G., Lang,D.B., Cox,D.R., Masters,S.B. and Myers,R.M.
  TITLE     Sequence-Tagged Sites for Human Chromosome 4
  JOURNAL   Unpublished (1992) see COMMENT for author address
  STANDARD  full automatic
COMMENT     PCR components: 25 ng of human genomic DNA, 10 pmol of each
            oligonucleotide, 200 micro-M dNTPs, 0.25 U Taq  polymerase (Cetus)
            in 10 micro-l of 50 mM KCl-20 mM  Tris-HCl, pH 8.3 (at room temp),
            2.5 mM MgCl-2.  Initial denaturation at 94degC for 1.5 min, then 30
            cycles of  94degC for 15 sec, 62degC for 23 sec, and 72degC for 30
            sec, followed by a final extension at 72degC for 3.5 min, using a
            Perkin-Elmer 9600 thermocycler. PCR-amplified product size 183 bp.
            STS sequence contains a simple sequence tandem repeat (CA).
            Sequence submitted by:
            Human Genome Mapping Center
            Box 0925
            University of California San Francisco
            San Francisco, CA 94143-0925  USA
            Phone: (415) 502-1612   Fax: (415) 476-8391
            e-mail: hgmcprobes@cgl.ucsf.edu
FEATURES             Location/Qualifiers
     misc_feature    65..88
                     /label=Primer1
     misc_feature    complement(224..247)
                     /label=Primer2
BASE COUNT      164 a     90 c     80 g    126 t      8 others
ORIGIN
        1 gatctttggg atgtgggagg aaaccagagt ccctggagna aacccacaca gacatgggga
       61 gaatgtacaa actccacata gacagtagtc ctggctgaga atcaggtttt tttctcatta
      121 atggtataat gaaatggcct tattcaagga cctcctgaac ctcccacact ctgatataac
      181 cctccaacac acacacacac acacacacac actcgntgaa atcattacca tagagactcc
      241 ttgtccttga taagaatgtg tctctctcac tgaaataggg ggaaagaaag ttgagaatta
      301 aagcctagag gcctaaaatc taccaatagg atttgnccac aaatataata ttacattata
      361 ataatatata catatatgaa tatgttggtn ttatataaca tcaatatatt aaatataata
      421 aagcaatcta ntcattatnt tttgattggn ggtngggata atacgcat
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human chromosome 4 sequence-tagged site STS4-252
Date: 21 Aug 92 10:08:15 GMT
Distribution: bionet
Checksum: 04359     3

LOCUS       HUM4STS252    369 bp ds-DNA             PRI       21-AUG-1992
DEFINITION  Human chromosome 4 sequence-tagged site STS4-252
ACCESSION   L00766
KEYWORDS    STS; human chromosome 4; sequence tagged site.
SOURCE      Homo sapiens, clone p4-197 from a chromosome 4 plasmid library;
            vector pBluescript II KS + (Stratagene)
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 369)
  AUTHORS   Goold,R.D., diSibio,G., Dugaiczyk,A., Smith,K.A., Xu,H.,
            Magrane,G., Lang,D.B., Cox,D.R., Masters,S.B. and Myers,R.M.
  TITLE     Sequence-Tagged Sites for Human Chromosome 4
  JOURNAL   Unpublished (1992) see COMMENT for author address
  STANDARD  full automatic
COMMENT     PCR components: 25 ng of human genomic DNA, 10 pmol of each
            oligonucleotide, 200 micro-M dNTPs, 0.25 U Taq  polymerase (Cetus)
            in 10 micro-l of 50 mM KCl-20 mM  Tris-HCl, pH 8.3 (at room temp),
            2.5 mM MgCl-2.  Initial denaturation at 94degC for 1.5 min, then 30
            cycles of  94degC for 15 sec, 62degC for 23 sec, and 72degC for 30
            sec, followed by a final extension at 72degC for 3.5 min, using a
            Perkin-Elmer 9600 thermocycler. PCR-amplified product size 234 bp.
            Sequence submitted by:
            Human Genome Mapping Center
            Box 0925
            University of California San Francisco
            San Francisco, CA 94143-0925  USA
            Phone: (415) 502-1612   Fax: (415) 476-8391
            e-mail: hgmcprobes@cgl.ucsf.edu
FEATURES             Location/Qualifiers
     misc_feature    25..49
                     /label=Primer1
     misc_feature    complement(235..258)
                     /label=Primer2
BASE COUNT      110 a     74 c     36 g    146 t      3 others
ORIGIN
        1 gatcaaaata atgactctca gatatgcatt attaacacag ttgtccttct tgagttttaa
       61 atttatatcc aactgtcttc taaatatttc tacgatagct aaaattacaa aaacacaaac
      121 ctccactcac cttctatgcc tcaaatgttt tctgtttttt ttttctctag atagttttaa
      181 cctgcaacta tctgagcaat aaattcaaat attgcatttg actctatgtt tattctccta
      241 cctccaaaag attactacat tttatcaaat tngggatctt agataacttt gntgcttttc
      301 atatagccat cactctagtt ggctacccta tctctggctt atttatttna atatactcat
      361 aaatttgtt
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: 57-VK
Date: 13 Aug 92 10:00:53 GMT
Distribution: bionet
Checksum: 17995     3

LOCUS       MUSD12733     322 bp ss-mRNA            ROD       13-AUG-1992
DEFINITION  57-VK
ACCESSION   D12733
KEYWORDS    .
SOURCE      Mus musculus (strain BALB/c) mRNA.
  ORGANISM  Mus musculus
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Rodentia; Myomorpha; Muridae; Murinae.
REFERENCE   1  (bases 1 to 322)
  AUTHORS   Sawada,J., Mizusawa,S., Terao,T., Naito,M. and Kurosawa,Y.
  TITLE     Molecular characterization of monoclonal anti-steroid antibodies:
            primary structures of the variable regions of seven antibodies
            specific for 17alpha-hydroxyprogesterone or 11-deoxycortisol and
            their pH-reactivity profiles
  JOURNAL   Mol. Immunol. 28, 1063-1072 (1991)
  STANDARD  full automatic
COMMENT     Submitted (27-JUL-1992) to DDBJ by:
            Jun-ichi Sawada
            Dept. of Biochem. and Immunochem.
            National Institute of Hygienic Sciences
            1-18-1 Kamiyoga
            Setagaya, Tokyo 158
            Japan
            Phone:  03-3700-1141 x240
            Fax:    03-3707-6950
FEATURES             Location/Qualifiers
     CDS             <1..>322
                     /product="'Ig kappa chain precursor'"
                     /codon_start=1
                     /translation="SIVMTQTPKFLLIRAGDRVTMTCKASQSARNGVAWYQQKPGQSP
                     KLLIYYTSDRYTGVPDRFTGSGYGTDFTFTISTVQAEDVAVYFCQQVYSSPYTFGGGT
                     KLEIK"
     mat_peptide     1..>322
                     /note="'VJ region (1-322)'"
                     /product="'Ig kappa chain'"
                     /codon_start=1
BASE COUNT       86 a     76 c     84 g     76 t
ORIGIN
        1 agtattgtga tgacccagac tcccaaattc ctgcttataa gagcaggaga cagggtcacc
       61 atgacctgca aggccagtca gagtgcgaga aatggtgtag cttggtacca acagaagcca
      121 gggcagtctc ctaaactgct gatatactat acatccgatc gctacactgg agtccctgat
      181 cgcttcactg gcagtggata tgggacggat ttcactttca ccatcagcac tgtgcaggct
      241 gaagacgtgg cagtttattt ctgtcagcag gtttatagtt ctccgtacac gttcggaggg
      301 gggaccaagc tggaaataaa ac
//