HDTenEightyP/NetNews huggingface dataset:
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human chromosome 4 sequence-tagged site STS4-267
Date: 21 Aug 92 10:08:25 GMT
Distribution: bionet
Checksum: 18074     3

LOCUS       HUM4STS267    458 bp ds-DNA             PRI       21-AUG-1992
DEFINITION  Human chromosome 4 sequence-tagged site STS4-267
ACCESSION   L00771
KEYWORDS    STS; human chromosome 4; sequence tagged site.
SOURCE      Homo sapiens, clone p4-203 from a chromosome 4 plasmid library;
            vector pBluescript II KS + (Stratagene)
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 458)
  AUTHORS   Goold,R.D., diSibio,G., Dugaiczyk,A., Smith,K.A., Xu,H.,
            Magrane,G., Lang,D.B., Cox,D.R., Masters,S.B. and Myers,R.M.
  TITLE     Sequence-Tagged Sites for Human Chromosome 4
  JOURNAL   Unpublished (1992) see COMMENT for author address
  STANDARD  full automatic
COMMENT     PCR components: 25 ng of human genomic DNA, 10 pmol of each
            oligonucleotide, 200 micro-M dNTPs, 0.25 U Taq  polymerase (Cetus)
            in 10 micro-l of 50 mM KCl-20 mM  Tris-HCl, pH 8.3 (at room temp),
            2.5 mM MgCl-2.  Initial denaturation at 94degC for 1.5 min, then 30
            cycles of  94degC for 15 sec, 62degC for 23 sec, and 72degC for 30
            sec, followed by a final extension at 72degC for 3.5 min, using a
            Perkin-Elmer 9600 thermocycler. PCR-amplified product size 179 bp.
            Sequence submitted by:
            Human Genome Mapping Center
            Box 0925
            University of California San Francisco
            San Francisco, CA 94143-0925  USA
            Phone: (415) 502-1612   Fax: (415) 476-8391
            e-mail: hgmcprobes@cgl.ucsf.edu
FEATURES             Location/Qualifiers
     misc_feature    110..133
                     /label=Primer1
     misc_feature    complement(265..288)
                     /label=Primer2
BASE COUNT      144 a    113 c    103 g     97 t      1 others
ORIGIN
        1 gatcaggaca gaagggaaat gcttgccaga tatcggaact gccagtggca acagcattca
       61 catgttgcca ggtccttgac cttaggtttt caccactcaa aagggacccc acagagaaga
      121 ctgaaatctt gaggtctgag aaccatggat gtgggagcca gaggaaaaga ccatgataac
      181 ccatgtccac catcaatggt actcatgcta tgaaatcccc cttggggtaa aaatctccac
      241 cagaacacat aggtggtgac aaatctcatt ggaccatcct aatctctgga tttaaaacga
      301 cctccttatg catccataag gagtctgtca aaggtcaaaa ggtgacctag tttgtatcca
      361 tcagtggccc agatggcagt gatcacagct gaagaagcta cacagagagt cttgtacccg
      421 cccagaacca gagtcaatgn accctgcaca agttacaa
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Figure 1. Genomic DNA sequence containing the 5' end of the murine
Date: 19 Aug 92 10:02:42 GMT
Distribution: bionet
Checksum: 32761     3

LOCUS       M23333       1190 bp ds-DNA             UNA       19-AUG-1992
DEFINITION  Figure 1. Genomic DNA sequence containing the 5' end of the murine
            alpha-1(IV) and alpha-2(IV) collagen genes and cDNA sequence for a
            portion of the 7S domain of the alpha-2(IV) chain.
ACCESSION   M23333
KEYWORDS    .
SOURCE
  ORGANISM  Unclassified.
            Unclassified.
REFERENCE   1  (bases 1 to 1190)
  AUTHORS   Burbelo,P.D., Martin,G.R. and Yamada,Y.
  TITLE     alpha-1(IV) and alpha-2(IV) collagen genes are regulated by a
            bidirectional promoter and a shared enhancer
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 85, 9679-9682 (1988)
  STANDARD  unannotated staff_entry
REFERENCE   2  (sites)
  AUTHORS   Bruggeman,L.A., Burbelo,P.D., Yamada,Y. and Klotman,P.E.
  TITLE     A novel sequence in the type IV collagen promoter binds nuclear
            proteins from engelbreth-holm-swarm tumor
  JOURNAL   Oncogene 7, 1497-1502 (1992)
  STANDARD  full staff_review
BASE COUNT      195 a    375 c    416 g    204 t
ORIGIN
        1 cttcgcagct gctcggctgc gctcctcgtg gagcagaagg gcggcgaaga gcagcagaag
       61 ccagacgctg agccggggcc ccatggtggc gcgcccgggg gcggcagggg accagcagag
      121 acggcgggga aatgcagccc agttcccgtg ggccgcggcg aggtgctcag tctcggaggg
      181 cccgggcgtg agccctgctc accgtcccgg gtgcggtgac tctgcgcaga gacctgagcg
      241 cgccgcccga ggagctcccc aatttgttgg cgctgccccc tcccctcggc agtgcgcggg
      301 cggcgctcct ccaaggggag gaacctgcgg cgcgggtaag aggcggaggc tgcgcgccgc
      361 tcgggacttg gtgctcgcag ggagtccgaa cgccggggct ctgctgtccg cacccagctc
      421 ctcccgcgcc ccgattccac gagcccgctt ggctcgccaa tcccgggcaa gcaccagcaa
      481 ggcacgccgg ggccgagagt ccggcgccca cggaggcaga gccaggccgg cctcggccgc
      541 cggtgtgcca cgggccaacg cttcttcagg ggcccaatgc gcgcgggcag cagctcctga
      601 ccctgaggtg agagcgaccc ccgggaggca cagagagcag gccccaaagt cgcggacctg
      661 tagcgccgga gtaggctgag gctgcgcgct tccgttggat cggagctggg ggaataggga
      721 tcgcgctgca tgggctgagg agctccaggc agggcctacg gagaagaatc tccctttttc
      781 tgtctcctga tgggacagaa ctttggtcac cccgcaagga aacgagggac attatgcgga
      841 gtcagggtgc taagatgacg atgctctctc tgcctgccac ctttgctaac ccgctttgtc
      901 tcttgcctcc aggttaggaa gggatcgagc cgagtctaga gctcaaagac tgggatcatg
      961 gaccgagtgc ggttcaaagc gtctggccct cccctgcgag ggtaagtcgc cttctgcctg
     1021 gtctcctagg tgaaggggac cttgcatgca tatttcagga gttgacaagt tggggcccgc
     1081 atgttttggt cgctttctta ccctccctcc ctctcctgtg caggtggctg ctgctagcaa
     1141 ctgtgacagt gggactcctg gctcagagcg tcttgggggt aagtaccgct
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human polymorphic microsatellite DNA.
Date: 19 Aug 92 10:00:55 GMT
Distribution: bionet
Checksum: 06444     2

LOCUS       HUM2PLYSAT    332 bp ds-DNA             PRI       19-AUG-1992
DEFINITION  Human polymorphic microsatellite DNA.
ACCESSION   M99142
KEYWORDS    microsatellite polymorphism.
SOURCE      Homo sapiens DNA.
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 332)
  AUTHORS   Bowcock,A.M.Ph.D.., Osborne-Lawrence,S.L., Barnes,R.I.,
            Chakravarti,A., Washington,S. and Dunn,C.
  TITLE     A linkage map of human chromosome 13q containing microsatellite
            polymorphisms
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     satellite       1..332
                     /note="map=13q; awaiting approval /map qualifier"
BASE COUNT      101 a     69 c     38 g    117 t      7 others
ORIGIN
        1 tactctcatt cccatgaatt ggttctgatc agcattcttc tattatgaaa tcgaaaacgc
       61 ttgaggtctc tatggaaagt tgtacataga gctgagaaat cacaacagag atgtaactca
      121 taatcctttt tcttatttaa atttaatcac agacacacac acacacacac acacacacac
      181 acacacacaa ttttccttgt ttcctggtac agagttctga aaacctatag aatctctntg
      241 anattagtaa gtcgtctttt tctattttat taactttttt nncttacatt gttncttttt
      301 ttnttttnct ggtaagaaca cctaaaatct ac
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Stereum complicatum ribosomal RNA (62LR0R) large subunit, partial.
Date: 19 Aug 92 10:03:57 GMT
Distribution: bionet
Checksum: 37650     2

LOCUS       STQRRLSB      228 bp ss-rRNA            RNA       19-AUG-1992
DEFINITION  Stereum complicatum ribosomal RNA (62LR0R) large subunit, partial.
ACCESSION   M98669
KEYWORDS    large subunit ribosomal RNA.
SOURCE      Stereum complicatum (library: Duke culture collection 783) cDNA to
            rRNA.
  ORGANISM  Stereum complicatum
            Eukaryota; Fungi; Amastigomycota; Basidiomycetes.
REFERENCE   1  (bases 1 to 228)
  AUTHORS   Hibbett,D.S. and Vilgalys,R.
  TITLE     Phylogenetic relationships of the basidiomycete genus Lentinus
            inferred from molecular and morphological characters
  JOURNAL   Unpublished (1992)
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     rRNA            <1..>228
                     /note="Aligns to region between positions 70 to 306
                     inSaccharomyces cerevisiae large subunit nuclear rRNA."
                     /product="large subunit ribosomal RNA"
                     /citation=[1]
BASE COUNT       53 a     48 c     69 g     58 t
ORIGIN
        1 ccctagtaac gcgagtgaag cggaaaagct caaatttaaa atctggcggt cttctggccg
       61 tccgagttgt agtctggaga agcgttttcc gcgttggacc gtgtacaagt ttcctggaac
      121 ggagcgtcat agagggtgag aatcccgtct ttgacacgga ccccaatgct tttgtgatgc
      181 gctctcaaag agtcgagttg tttgggaatg cagccaaaat gggtggtg
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human chromosome 4 sequence-tagged site STS4-359
Date: 21 Aug 92 10:09:24 GMT
Distribution: bionet
Checksum: 55545     3

LOCUS       HUM4STS359    434 bp ds-DNA             PRI       21-AUG-1992
DEFINITION  Human chromosome 4 sequence-tagged site STS4-359
ACCESSION   L00816
KEYWORDS    STS; human chromosome 4; sequence tagged site.
SOURCE      Homo sapiens, clone p4-301 from a chromosome 4 plasmid library;
            vector pBluescript II KS + (Stratagene)
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 434)
  AUTHORS   Goold,R.D., diSibio,G., Dugaiczyk,A., Smith,K.A., Xu,H.,
            Magrane,G., Lang,D.B., Cox,D.R., Masters,S.B. and Myers,R.M.
  TITLE     Sequence-Tagged Sites for Human Chromosome 4
  JOURNAL   Unpublished (1992) see COMMENT for author address
  STANDARD  full automatic
COMMENT     PCR components: 25 ng of human genomic DNA, 10 pmol of each
            oligonucleotide, 200 micro-M dNTPs, 0.25 U Taq  polymerase (Cetus)
            in 10 micro-l of 50 mM KCl-20 mM  Tris-HCl, pH 8.3 (at room temp),
            2.5 mM MgCl-2.  Initial denaturation at 94degC for 1.5 min, then 30
            cycles of  94degC for 15 sec, 62degC for 23 sec, and 72degC for 30
            sec, followed by a final extension at 72degC for 3.5 min, using a
            Perkin-Elmer 9600 thermocycler. PCR-amplified product size 181 bp.
            Sequence submitted by:
            Human Genome Mapping Center
            Box 0925
            University of California San Francisco
            San Francisco, CA 94143-0925  USA
            Phone: (415) 502-1612   Fax: (415) 476-8391
            e-mail: hgmcprobes@cgl.ucsf.edu
FEATURES             Location/Qualifiers
     misc_feature    72..97
                     /label=Primer1
     misc_feature    complement(229..252)
                     /label=Primer2
BASE COUNT      117 a    115 c     99 g     96 t      7 others
ORIGIN
        1 gatcctgtct caaaaacaca cacacacaca cacacacaca cacacacaca cgcacgcaca
       61 cacaaaatta cctcaaaact tggttgttga aaacagtaat atgcctttat aatctcacag
      121 tttntgtggg tcaggaattt gagagtatct gagctgggtg gttctagccc aaggtctctc
      181 ctgaggcttt agtcaggtca ccagccagga ccgcagtcat ccaaagtcct ctgaagatgt
      241 gaattgacgc aggcccattc acatggntag tcggttggag ccagctgtca caggaggntt
      301 cacctcctca ctcaccgacc tccctgtagg gtttcttcag tgtcctgatg tcatagnggg
      361 cagcttccct caggggaggg aatccaagca agagcaggca gaggnaggaa tntctcttag
      421 gacctggntc acaa
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Odocoileus virginianus texanus cytochrome b gene, 3' end.
Date: 19 Aug 92 10:02:55 GMT
Distribution: bionet
Checksum: 64865     2

LOCUS       ODOETXCB      307 bp ds-DNA             ORG       19-AUG-1992
DEFINITION  Odocoileus virginianus texanus cytochrome b gene, 3' end.
ACCESSION   M98493
KEYWORDS    cytochrome b.
SOURCE      Mitochondrion Odocoileus virginianus (chromosome Mitochondrion
            Odocoileus virginianus, sub_species texanus) adult liver DNA.
  ORGANISM  Mitochondrion Odocoileus virginianus
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Artiodactyla; Ruminantia; Pecora; Cervidae; Odocoileinae.
REFERENCE   1  (bases 1 to 307)
  AUTHORS   Carr,S.M. and Hughes,G.A.
  TITLE     The direction of hybridization between species of North American
            deer (Odocoileus) as inferred from mitochondrial cytochrome b
            sequences
  JOURNAL   J. Mammal. (1992) In press
  STANDARD  full staff_entry
FEATURES             Location/Qualifiers
     CDS             <1..298
                     /product="cytochrome b"
                     /codon_start=3
                     /translation="FGSLLGICLILQILTGLFLAMHYTSDTMTAFSSVTHICRDVNYG
                     WIIRYMHANGASMFFICLFMHVGRGLYYGSYTFLETWNIGVILLFTVMATAFVG"
BASE COUNT       88 a     75 c     47 g     97 t
ORIGIN
        1 acttcggctc tctgctagga atttgcttaa tcctacaaat tcttactggt ctattcctag
       61 caatacatta tacatccgac acaataacag cattttcctc tgtcactcac atctgccgag
      121 acgttaacta tggctgaatc attcgataca tacatgccaa tggagcatcc atattcttta
      181 tctgtctatt catacacgtg ggacgaggac tatactacgg atcatatact ttcttagaaa
      241 catgaaacat tggagtaatt ctcctattca cagttatagc cacagcattc gtagggtatg
      301 tcctacc
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Austrailian lungfish mitochondrion ribosomal RNA small subunit
Date: 21 Aug 92 10:12:24 GMT
Distribution: bionet
Checksum: 32481     2

LOCUS       NEEMTRRNA     303 bp ds-DNA             ORG       21-AUG-1992
DEFINITION  Austrailian lungfish mitochondrion ribosomal RNA small subunit
            gene, internal sequence.
ACCESSION   M87536
KEYWORDS    ribosomal RNA; ribosomal RNA small subunit.
SOURCE      Mitochondrion Neoceratodus foresteri (chromosome Mitochondrion
            Neoceratodus foresteri) DNA.
  ORGANISM  Mitochondrion Neoceratodus foresteri
            Eukaryota; Animalia; Chordata; Vertebrata; Osteichthyes;
            Sarcopterygii; Lepidosireniformes; Ceratodidae.
REFERENCE   1  (bases 1 to 303)
  AUTHORS   Meyer,A. and Dolven,S.I.
  TITLE     Molecules, fossils and the origin of tetrapods
  JOURNAL   J. Mol. Evol. 35, 102-113 (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     rRNA            <1..>303
                     /gene="ribosomal RNA small subunit"
BASE COUNT      106 a     70 c     63 g     64 t
ORIGIN
        1 agcttaaaac ccaaaggact tggcggtgcc tcacacccac ctagaggagc ctgttctaga
       61 accgataatc cacgttaaac ctcaccgctt cttgccacta ccgtctatat accaccgtcg
      121 ccagcttacc ccgtgagggt gaaaaagtaa gcacaattgg tacaaccaaa aacgtcaggt
      181 cgaggtgtag cgaatgaagc ggaaagaaat gggctacatt ttcttaaaag aatatacgaa
      241 agacctaatg aaacaaagtc agaaggtgga tttagcagta agaaaaacca agaatatttt
      301 tct
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human chromosome 4 sequence-tagged site STS4-180
Date: 21 Aug 92 10:07:25 GMT
Distribution: bionet
Checksum: 17409     3

LOCUS       HUM4STS180    427 bp ds-DNA             PRI       21-AUG-1992
DEFINITION  Human chromosome 4 sequence-tagged site STS4-180
ACCESSION   M95272
KEYWORDS    STS; human chromosome 4; sequence tagged site.
SOURCE      Homo sapiens, clone C4-175 from Los Alamos National Laboratory
            chromosome 4 cosmid library, plate 4-1-2R, pos1C; vector SuperCos 1
            (Stratagene)
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 427)
  AUTHORS   Goold,R.D., diSibio,G., Dugaiczyk,A., Smith,K.A., Xu,H.,
            Magrane,G., Lang,D.B., Cox,D.R., Masters,S.B. and Myers,R.M.
  TITLE     Sequence-Tagged Sites for Human Chromosome 4
  JOURNAL   Unpublished (1992) see COMMENT for author address
  STANDARD  full automatic
COMMENT     PCR components: 25 ng of human genomic DNA, 10 pmol of each
            oligonucleotide, 200 micro-M dNTPs, 0.25 U Taq  polymerase (Cetus)
            in 10 micro-l of 50 mM KCl-20 mM  Tris-HCl, pH 8.3 (at room temp),
            2.5 mM MgCl-2.  Initial denaturation at 94degC for 1.5 min, then 30
            cycles of  94degC for 15 sec, 64degC for 23 sec, and 72degC for 30
            sec, followed by a final extension at 72degC for 3.5 min, using a
            Perkin-Elmer 9600 thermocycler. PCR-amplified product size 200 bp.
            Sequence submitted by:
            Human Genome Mapping Center
            Box 0925
            University of California San Francisco
            San Francisco, CA 94143-0925  USA
            Phone: (415) 502-1612   Fax: (415) 476-8391
            e-mail: hgmcprobes@cgl.ucsf.edu
FEATURES             Location/Qualifiers
     misc_feature    68..92
                     /label=Primer1
     misc_feature    complement(245..267)
                     /label=Primer2
BASE COUNT      106 a     76 c     91 g    135 t     19 others
ORIGIN
        1 aaaagttcca tgtgttggta aacttgaatt gcagtngtaa cattgttgag aggngggcct
       61 ttggaaggtg attgaatatt gaagtctctg cccttatgaa tggattaacg ctattatcat
      121 ggtagttgtt ttgttatcta gcaattggct ctgttataaa agtgagcttt ctcttgcatg
      181 agctgtctcc tgccctttca cctatctgat aggggntaat tcttgccaga tgccagtgcc
      241 atgntcttgg acttcccagt ctgtagaanc ataagccaaa taaacctctt ttctttataa
      301 attacacaat ctggggtatt ntgtcacagc agcancaaca ncannaatta nncacactan
      361 tacaggaggt caggggnctt tgtcaagtgn tggctaangg anacaattag tctgccngng
      421 tgttttt
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Papio hamadrayas MHC DQB1*03 gene, partial cds, clone Badqb103.
Date: 12 Aug 92 10:00:27 GMT
Distribution: bionet
Checksum: 50671     2

LOCUS       BABMHDQBB     246 bp ds-DNA             PRI       12-AUG-1992
DEFINITION  Papio hamadrayas MHC DQB1*03 gene, partial cds, clone Badqb103.
ACCESSION   M81284
KEYWORDS    major histocompatibility complex.
SOURCE      Papio hamadryas (individual_isolate Marcel) blood DNA.
  ORGANISM  Papio hamadryas
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Cercopithecidae;
            Cercopithecinae; Papionini.
REFERENCE   1  (bases 1 to 246)
  AUTHORS   Bontrop,R.E., Jonker,M., van Weeren,P., Kenter,M. and Otting,N.
  TITLE     MHC-DQB repertoire variation in hominoid and old world primate
            species
  JOURNAL   J. Immunol. 149, 461-470 (1992)
  STANDARD  full staff_entry
FEATURES             Location/Qualifiers
     CDS             <1..>246
                     /gene="MHC DQ-beta 1"
                     /label=exon 2
                     /codon_start=1
                     /translation="FQFKGMCYFTNGTGRVWSVDRYIYNREEYVRFDSDVGLYLPVTP
                     LGRSWAEYWNSQKEALESKRAELDRVCIHNYQLELGTT"
BASE COUNT       58 a     59 c     85 g     44 t
ORIGIN
        1 ttccagttta agggcatgtg ctacttcacc aacgggacag ggcgcgtgtg gagtgtagac
       61 agatacatct ataaccgaga ggagtacgtg cgcttcgaca gcgacgtggg gttgtacctg
      121 ccggtgacgc cgctggggcg gtcttgggcc gagtactgga acagccagaa ggaagccctg
      181 gagagcaaac gggcggagtt ggacagggtg tgcatacaca actaccagtt agagctcggc
      241 acgacc
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human chromosome 4 sequence-tagged site STS4-133
Date: 21 Aug 92 10:06:50 GMT
Distribution: bionet
Checksum: 11794     3

LOCUS       HUM4STS133    409 bp ds-DNA             PRI       21-AUG-1992
DEFINITION  Human chromosome 4 sequence-tagged site STS4-133
ACCESSION   M95249
KEYWORDS    STS; human chromosome 4; sequence tagged site.
SOURCE      Homo sapiens, clone C4-152 from Los Alamos National Laboratory
            chromosome 4 cosmid library, plate 4-1-2R, pos 2A; vector SuperCos
            1 (Stratagene)
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 409)
  AUTHORS   Goold,R.D., diSibio,G., Dugaiczyk,A., Smith,K.A., Xu,H.,
            Magrane,G., Lang,D.B., Cox,D.R., Masters,S.B. and Myers,R.M.
  TITLE     Sequence-Tagged Sites for Human Chromosome 4
  JOURNAL   Unpublished (1992) see COMMENT for author address
  STANDARD  full automatic
COMMENT     PCR components: 25 ng of human genomic DNA, 10 pmol of each
            oligonucleotide, 200 micro-M dNTPs, 0.25 U Taq  polymerase (Cetus)
            in 10 micro-l of 50 mM KCl-20 mM  Tris-HCl, pH 8.3 (at room temp),
            2.5 mM MgCl-2.  Initial denaturation at 94degC for 1.5 min, then 30
            cycles of  94degC for 15 sec, 62degC for 23 sec, and 72degC for 30
            sec, followed by a final extension at 72degC for 3.5 min, using a
            Perkin-Elmer 9600 thermocycler. PCR-amplified product size 187 bp.
            Sequence submitted by:
            Human Genome Mapping Center
            Box 0925
            University of California San Francisco
            San Francisco, CA 94143-0925  USA
            Phone: (415) 502-1612   Fax: (415) 476-8391
            e-mail: hgmcprobes@cgl.ucsf.edu
FEATURES             Location/Qualifiers
     misc_feature    22..44
                     /label=Primer1
     misc_feature    complement(184..208)
                     /label=Primer2
BASE COUNT      112 a     81 c     86 g     97 t     33 others
ORIGIN
        1 cnggnggagn caccagtggg gagttgggta caaacagggt gtgagtgcca tggaaaggat
       61 caggcctcac tantctagag aaaccagcca atgaaaanta gcaacagact gaaataatgt
      121 caacacttgg aagggaaagg agcacaagga agactcactt ctaaatatac aacagncagt
      181 tcacattcct tgtcaaaata ctgagctccc tccgtgacga ctgangttga nagagcanca
      241 caattgtgtt ttgggtntgc atatttagaa tntaccaagt gaggatgncg caccntgggg
      301 aanaaangac ttgncatgnc ataggnttnc attgctgttt gcccncnnct tattttaact
      361 tactctnctt ccncctnctt nnnttcnttt attattccat cgncagnnn
//