From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Mouse mRNA for mouse melanoma antigen, complete cds.
Date: 9 Jan 93 05:47:09 GMT
Distribution: bionet
Checksum: 35568 7

LOCUS       MUSH52EP     2767 bp ss-mRNA            ROD       09-JAN-1993
DEFINITION  Mouse mRNA for mouse melanoma antigen, complete cds.
ACCESSION   D10049
KEYWORDS    Murine endogenous leukemia virus; env polyprotein;
            mouse melanoma antigen; tumor associated antigen.
SOURCE      Mouse (strain C57BL/6) malignant melanoma cell, cell line B16, cDNA
            to mRNA.
  ORGANISM  Mus musculus
            Eukaryota; Animalia; Chordata; Mammalia; Rodentia; Muridae.
REFERENCE   1  (bases 1 to 2767)
  AUTHORS   Hayashi,H., Matsubara,H., Yokota,T., Kuwabara,I., Kanno,M.,
            Koseki,H., Isono,K., Asano,T. and Taniguchi,M.
  TITLE     Molecular cloning and characterization of the gene encoding mouse
            melanoma antigen by cDNA library transfection
  JOURNAL   J. Immunol. 149, 1223-1229 (1992)
  STANDARD  full automatic
COMMENT     Submitted (06-Dec-1991) to DDBJ by: Hideki Hayashi Division of
            Molecular Immunology Center for Neurobiology and Immunology Chiba
            University, School of Medicine 1-8-1 Inohana Chiba 280 Japan Phone:
             0472-22-7171 x2156 Fax:    0472-27-1498.
FEATURES             Location/Qualifiers
     mat_peptide     368..1684
                     /product="gp70"
                     /codon_start=1
     mat_peptide     1685..2224
                     /product="p15E"
                     /codon_start=1
     LTR             2317..2699
                     /note="U3"
     LTR             2700..2767
                     /note="R"
     CDS             221..2284
                     /gene="H52"
                     /product="env polyprotein"
                     /codon_start=1
                     /translation="MDTRRPRQGSDHTPDKTIMESTTLSKPFKNQVNPWGPLIVLLIL
                     GGVNPVALGNSPHQVFNLSWEVTNGDRETVWAITGNHPLWTWWPDLTPDLCMLALHGP
                     SYWGLEYRAPFSPPPGPPCCSGSSDSTSGCSRDCEEPLTSYTPRCNTAWNRLKLSKVT
                     HAHNEGFYVCPGPHRPRWARSCGGPESFYCASWGCETTGRASWKPSSSWDYITVSNNL
                     TSDQATPVCKGNKWCNSLTIRFTSFGKQATSWVTGHWWGLRLYVSGHDPGLIFGIRLK
                     ITDSGPRVPIGPNPVLSDRRPPSRPRPTRSPPPSNSTPTETPLTLPEPPPAGVENRLL
                     NLVKGAYQALNLTSPDKTQECWLCLVSGPPYYEGVAVLGTYSNHTSAPANCSVASQHK
                     LTLSEVTGQGLCIGAVPKTHQVLCNTTQKTSDGSYYLVAPTGTTWACSTGLTPCISTT
                     ILNLTTDYCVLVELWPRVTYHSPSYVYHQFERRAKYKREPVSLTLALLLGGLTMGGIA
                     AGVGTGTTALVATQQFQQLQAAMHDDLKEVEKSITNLEKSLTSLSEVVLQNRRGLDLL
                     FLKEGGLCAALKEECCFYADHTGLVRDSMAKLRERLSQRQKLFESQQGWFEGLFNKSP
                     WFTTLISTIMGPLIILLLILLFGPCILNRLVQFIKDRISVVQALVLTQQYHQLKTIGD
                     CKSRE"
BASE COUNT      732 a    810 c    628 g    597 t
ORIGIN      
        1 acttacaggc cctccaaacg gtgcagcgag aaatttggaa accactggcc gaggcctacc
       61 gggaccgact agaccaacca gtgataccgc accccttccg gattggagac tccgtgtggg
      121 tgcgccggca ccagaccaaa aacttagaac ctcgctggaa gggaccctac accgtcctac
      181 tgaccacccc caccgctctc aaggtagacg gcatctctgc atggatacac gccgcccacg
      241 tcaaggcagc gaccacaccc ccgataaaac catcatggag agtacaacgc tctcaaaacc
      301 ctttaaaaat caggttaacc cgtggggccc cctaattgtc cttctgattc tcggaggggt
      361 caaccccgtt gcgttgggaa acagccccca ccaggttttt aacctctcct gggaagtgac
      421 taatggagac cgagaaacgg tgtgggcaat aaccggcaat caccctctgt ggacttggtg
      481 gcctgacctc acaccagatc tctgtatgtt ggccctccac gggccgtcct attggggcct
      541 agaatatcgg gctccttttt ctcctccccc ggggcccccc tgctgttcag gaagcagcga
      601 ctccacgtca ggctgttcca gagattgtga ggagcccctg acttcatata ctccccggtg
      661 caatacggcc tggaacagac ttaagttatc taaagtgaca catgcacaca atgaaggatt
      721 ctatgtctgc cccgggccac atcgcccccg gtgggcccgg tcgtgtggtg gtccagaatc
      781 cttctattgt gcctcttggg gctgcgaaac cacaggccga gcatcctgga aaccatcctc
      841 gtcctgggac tacatcacag taagcaacaa tctaacctca gaccaggcaa ccccagtatg
      901 caaaggtaat aagtggtgca actccttaac tatccggttc acgagctttg gaaaacaggc
      961 cacctcctgg gtcacaggcc attggtgggg attgcgccta tacgtctctg gacatgaccc
     1021 agggctcatc tttgggatcc gacttaaaat tacagactcg gggccccggg tcccaatagg
     1081 gccaaacccc gtcttgtcag accgacgacc accttcccgg cctagaccca ccagatctcc
     1141 cccgccttca aactccaccc caaccgagac acccctcacc ctccccgaac ccccgccagc
     1201 gggagtcgaa aaccgattgt taaatctagt aaaaggagcc taccaagccc tcaacctcac
     1261 cagtcctgat aaaacccaag agtgctggtt atgcctagta tcgggacccc catactacga
     1321 gggggttgcc gtcctaggta cctactccaa ccatacttct gccccagcta actgctctgt
     1381 ggcctctcaa cacaaattga ccttgtccga agtgaccgga cagggactct gcataggagc
     1441 ggtccctaaa acccatcaag tcttgtgtaa taccacccaa aagacaagcg atgggtccta
     1501 ctatttggtc gctcccacag gaactacctg ggcttgtagt actggactca ctccctgtat
     1561 ctcaaccacc atacttaacc tcaccaccga ttactgtgtc ctggtcgagc tttggccaag
     1621 ggtgacctac cattccccta gttatgttta ccaccaattt gaaagacgag ccaaatataa
     1681 aagagaaccc gtctcactaa ctctggccct actattagga ggactcacta tgggcggaat
     1741 tgccgctgga gtgggaacag ggactaccgc cctagtggcc actcagcagt tccaacaact
     1801 ccaggctgcc atgcacgatg accttaaaga agttgaaaag tccatcacta atctagaaaa
     1861 atctttgacc tccttgtccg aagtagtgtt acagaatcgt agaggcctag atctactatt
     1921 cctaaaagag ggaggtttgt gtgctgcctt aaaagaagaa tgctgtttct atgccgacca
     1981 cacaggattg gtacgggata gcatggccaa acttagagaa agattgagtc agagacaaaa
     2041 gctctttgaa tcccaacaag ggtggtttga agggctgttt aataagtccc cttggttcac
     2101 caccctgata tccaccatca tgggtcccct gataatcctc ttgttaattt tactctttgg
     2161 gccttgtatt ctcaatcgcc tggtccagtt tatcaaagac aggatttcgg tagtgcaggc
     2221 cctggttctg actcaacaat atcatcaact taagacaata ggagattgta aatcacgtga
     2281 ataaaagatt ttattcagtt tacagaaaga ggggggaatg aaagacccct tcataaggct
     2341 tagccagcta actgcagtaa cgccattttg caaggcatgg gaaaatacca gagctgatgt
     2401 tctcagaaaa acaagaacaa ggaagtacag agaggctgga aagtaccggg actagggcca
     2461 aacaggatat ctgtggtcaa gcactagggc cccggcccag ggccaagaac agatggtccc
     2521 cagaaatagc taaaacaaca acagtttcaa gagacccaga aactgtctca aggttcccca
     2581 gatgaccggg gatcaacccc aagcctcatt taaactaacc aatcagctcg cttctcgctt
     2641 ctgtacccgc gcttattgct gcccagctct ataaaaaggg taagaacccc acactcggcg
     2701 cgccagtcct ccgatagact gagtcgcccg ggtacccgtg tatccaataa agccttttgc
     2761 tgttgca
//