HDTenEightyP/NetNews huggingface dataset:
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human chromosome 4 sequence-tagged site STS4-414
Date: 21 Aug 92 10:10:05 GMT
Distribution: bionet
Checksum: 00913     3

LOCUS       HUM4STS414    516 bp ds-DNA             PRI       21-AUG-1992
DEFINITION  Human chromosome 4 sequence-tagged site STS4-414
ACCESSION   L00846
KEYWORDS    STS; human chromosome 4; sequence tagged site.
SOURCE      Homo sapiens, clone p4-355 from a chromosome 4 plasmid library;
            vector pBluescript II KS + (Stratagene)
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 516)
  AUTHORS   Goold,R.D., diSibio,G., Dugaiczyk,A., Smith,K.A., Xu,H.,
            Magrane,G., Lang,D.B., Cox,D.R., Masters,S.B. and Myers,R.M.
  TITLE     Sequence-Tagged Sites for Human Chromosome 4
  JOURNAL   Unpublished (1992) see COMMENT for author address
  STANDARD  full automatic
COMMENT     PCR components: 25 ng of human genomic DNA, 10 pmol of each
            oligonucleotide, 200 micro-M dNTPs, 0.25 U Taq  polymerase (Cetus)
            in 10 micro-l of 50 mM KCl-20 mM  Tris-HCl, pH 8.3 (at room temp),
            2.5 mM MgCl-2.  Initial denaturation at 94degC for 1.5 min, then 30
            cycles of  94degC for 15 sec, 66degC for 23 sec, and 72degC for 30
            sec, followed by a final extension at 72degC for 3.5 min, using a
            Perkin-Elmer 9600 thermocycler. PCR-amplified product size 177 bp.
            STS sequence contains a simple sequence tandem repeat (CA).
            Sequence submitted by:
            Human Genome Mapping Center
            Box 0925
            University of California San Francisco
            San Francisco, CA 94143-0925  USA
            Phone: (415) 502-1612   Fax: (415) 476-8391
            e-mail: hgmcprobes@cgl.ucsf.edu
FEATURES             Location/Qualifiers
     misc_feature    135..160
                     /label=Primer1
     misc_feature    complement(288..311)
                     /label=Primer2
BASE COUNT      136 a    138 c     91 g    124 t     27 others
ORIGIN
        1 gatcnctcca ccttgacctc ccaaagtgct ggccattaca ggcgtgagcc accgcacctg
       61 gcctcaagct attgtattag aagaggactt ttcaaattat tttattaacc ttcatcccct
      121 atctattaga aaccaagtgt attaatcaag tttctccaga ggaatggaac caaaaggata
      181 cacacacaca cacacacaca cacacacaca cacacacacc tcacatatgt atacttacat
      241 taatatnttt ctcacanata ttcnncatac tataganana natgagaatt ggctcatgta
      301 attatggagg cccagaagtc cccccatctn ccanatncna gctagagnnc ccgggnanct
      361 ggtggtgtga ttcagtctga gtctgnnggc cccagnnctg ggcacttcag tntgtngggc
      421 agnagaagat gtatttccca nctcccggag agagnggatt ttcccntccc ccnccttttc
      481 actctattca ggccctcacc cggattgggt gatgcc
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Polyporus squamosus ribosomal RNA (9LR0R) large subunit, partial.
Date: 19 Aug 92 10:03:32 GMT
Distribution: bionet
Checksum: 23365     2

LOCUS       POZRRLSG      235 bp ss-rRNA            RNA       19-AUG-1992
DEFINITION  Polyporus squamosus ribosomal RNA (9LR0R) large subunit, partial.
ACCESSION   M98643
KEYWORDS    large subunit ribosomal RNA.
SOURCE      Polyporus squamosus (library: Duke Herbarium SAR 89-468) cDNA to
            rRNA.
  ORGANISM  Polyporus squamosus
            Eukaryota; Fungi; Amastigomycota; Basidiomycetes.
REFERENCE   1  (bases 1 to 235)
  AUTHORS   Hibbett,D.S. and Vilgalys,R.
  TITLE     Phylogenetic relationships of the basidiomycete genus Lentinus
            inferred from molecular and morphological characters
  JOURNAL   Unpublished (1992)
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     rRNA            <1..>235
                     /note="Approximately aligns to region between positions 70
                     to 306in Saccharomyces cerevisiae large subunit nuclear
                     rRNA."
                     /product="large subunit ribosomal RNA"
                     /citation=[1]
BASE COUNT       57 a     44 c     69 g     65 t
ORIGIN
        1 gattccccta gtaactgcga gtgaagcggg aaaagctcaa atttaaaatc tggtggcctt
       61 tggtcatccg agttgtagtc tggagaagtg ctttccgcgt tggaccgtgt ataagtctct
      121 tggaatagag cgtcatagag ggtgagaatc ccgtctttga cacggactac cagtgctttg
      181 tgatgcgctc tcaaagagtc gagttgtttg ggaatgcagc tcaaaatggg tggta
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human immunodeficiency virus type 2 (FOENVA88) envelope protein
Date: 18 Aug 92 10:01:37 GMT
Distribution: bionet
Checksum: 28922     2

LOCUS       HIVENVA88     453 bp ss-RNA             VRL       18-AUG-1992
DEFINITION  Human immunodeficiency virus type 2 (FOENVA88) envelope protein
            fragment.
ACCESSION   M87086
KEYWORDS    envelope protein.
SOURCE      Human immunodeficiency virus type 2 uncultured blood mononuclear
            cells (11/89 sample) RNA.
  ORGANISM  Human immunodeficiency virus type 2
            Viridae; ss-RNA enveloped viruses; Positive strand RNA virus;
            Retroviridae; Lentivirinae.
REFERENCE   1  (bases 1 to 453)
  AUTHORS   Gao,F., Yue,L., White,A.T., Pappas,P.G., Barchue,J., Hanson,A.P.,
            Greene,B.M., Sharp,P.M., Shaw,G.M. and Hahn,B.H.
  TITLE     Human infection by genetically-diverse SIVsm-related HIV-2 in West
            Africa
  JOURNAL   Nature (1992) In press
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     misc_feature    1..453
                     /note="PCR derived HIV-2 envelope fragment"
                     /gene="env"
                     /note="putative"
                     /citation=[1]
BASE COUNT      163 a     77 c    102 g    111 t
ORIGIN
        1 gacgtggtca agagacaaca agaattgttg cgactgaccg tctgggggac taaaaacctc
       61 cagactagag tcactgctat cgagaagtac ctaaaggacc aggcgcagct aaattcatgg
      121 ggatgcgcgt tcaggcaggt ctgccacacc actgtagaat ggccaaatag tactctcaca
      181 cctgactaga acaatatgac ttggcaggag tgggagagac aggttgattt cctagaggca
      241 aatataacac aattattaga ggaagcacaa attcaacaag aaaagaacat gtatgagtta
      301 caaaaactaa ataactggga tatatttggc aactggtttg accttacttc ttggattaag
      361 tatatacagt atggagtctt catagtagta ggtataatac tgttaagaat aataatctat
      421 gtagtacaaa tgttagctaa gttaagtaaa aaa
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Methanosarcina thermophila ferredoxin (fdxA) gene.
Date: 22 Aug 92 10:01:03 GMT
Distribution: bionet
Checksum: 32647     2

LOCUS       MESFDXAA      501 bp ds-DNA             BCT       22-AUG-1992
DEFINITION  Methanosarcina thermophila ferredoxin (fdxA) gene.
ACCESSION   M83187
KEYWORDS    fdxA gene; ferredoxin.
SOURCE      Methanosarcina thermophila (strain ) DNA.
  ORGANISM  Methanosarcina thermophila
            Prokaryotae; Archaeobacteria; Euryarchaeota; Methanomicrobiales;
            Methanosarcinaceae.
REFERENCE   1  (bases 1 to 501)
  AUTHORS   Clements,A.P. and Ferry,J.G.
  TITLE     cloning, nucleotide sequence, and transcriptional analyses of the
            gene encoding a ferredoxin from Methanosarcina thermophila
  JOURNAL   J. Bacteriol. 174, 5244-5250 (1992)
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     mat_peptide     239..415
                     /function="electron acceptor from CO dehydrogenase"
                     /product="ferredoxin"
                     /gene="fdxA"
                     /evidence=EXPERIMENTAL
                     /codon_start=1
                     /citation=[1]
BASE COUNT      150 a     84 c    102 g    165 t
ORIGIN
        1 gaattcgatt ttttacttct tcttttattt ataaattaaa tgatatattt taatccccgg
       61 ctagttgact ccatcctgat tttgaaggct gcattttgtt aaaaagcgat aagtttttat
      121 gtttatcctg tcaatggcgt tatgaccaaa cttttcgata acctatggaa gttttaaaaa
      181 cgagctgtac aatttccagg tatatatcga gtaacaaaaa ggagtgtgaa aaaatatgcc
      241 agcattagtt aatgcagatg aatgttctgg atgcggaagc tgtgtcgatg aatgcccctc
      301 tgaagcaatc acccttgatg aagagaaagg aatcgcagtt gtcgaccagg acgaatgcgt
      361 agagtgcggc gcatgtgaag aagcatgccc gaaccaggca attaaagtag aagagtaaaa
      421 cggcaattag ttggaataag ctctttattc cacttttctt tttccttttt atcaatcatt
      481 ttttcttttt cggaggtagc t
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: 7D7-VK
Date: 13 Aug 92 10:00:56 GMT
Distribution: bionet
Checksum: 14613     3

LOCUS       MUSD12735     334 bp ss-mRNA            ROD       13-AUG-1992
DEFINITION  7D7-VK
ACCESSION   D12735
KEYWORDS    .
SOURCE      Mus musculus (strain BALB/c) mRNA.
  ORGANISM  Mus musculus
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Rodentia; Myomorpha; Muridae; Murinae.
REFERENCE   1  (bases 1 to 334)
  AUTHORS   Sawada,J., Mizusawa,S., Terao,T., Naito,M. and Kurosawa,Y.
  TITLE     Molecular characterization of monoclonal anti-steroid antibodies:
            primary structures of the variable regions of seven antibodies
            specific for 17alpha-hydroxyprogesterone or 11-deoxycortisol and
            their pH-reactivity profiles
  JOURNAL   Mol. Immunol. 28, 1063-1072 (1991)
  STANDARD  full automatic
COMMENT     Submitted (27-JUL-1992) to DDBJ by:
            Jun-ichi Sawada
            Dept. of Biochem. and Immunochem.
            National Institute of Hygienic Sciences
            1-18-1 Kamiyoga
            Setagaya, Tokyo 158
            Japan
            Phone:  03-3700-1141 x240
            Fax:    03-3707-6950
FEATURES             Location/Qualifiers
     CDS             <1..>334
                     /product="'Ig kappa chain precursor'"
                     /codon_start=1
                     /translation="DIVLTQYPASLAVSLGQRATISCRASESVDNYGISFMNWFQQKP
                     GQPPKLLIYGASNQGSGVPARFTGSGSGTDFSLNIQPMEEDDTAMYFCQQTKEVSYTF
                     GGGTKVEIK"
     mat_peptide     1..>334
                     /note="'VJ region (1-334)'"
                     /product="'Ig kappa chain'"
                     /codon_start=1
BASE COUNT       88 a     83 c     89 g     74 t
ORIGIN
        1 gacattgtgc tgacccaata tccagcttcc ttggctgtgt ctctagggca gagggccacc
       61 atctcctgca gagccagcga aagtgttgat aattatggaa ttagttttat gaactggttc
      121 caacagaaac cgggacagcc acccaaactc ctcatctatg gtgcatccaa ccaaggatcc
      181 ggggtccctg ccaggtttac aggcagtggg tctgggacag acttcagcct caacatccag
      241 cctatggagg aggatgacac tgccatgtat ttctgtcagc aaactaagga ggtgtcgtac
      301 acgttcggag gggggaccaa ggtggaaata aaac
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Rattus norvegicus pore-forming calcium channel alpha-1 subunit
Date: 19 Aug 92 10:03:39 GMT
Distribution: bionet
Checksum: 55385     2

LOCUS       RATCCAS       285 bp ss-mRNA            ROD       19-AUG-1992
DEFINITION  Rattus norvegicus pore-forming calcium channel alpha-1 subunit
            mRNA, partial cds.
ACCESSION   M99220
KEYWORDS    calcium channel alpha-1 subunit.
SOURCE      Rattus norvegicus (strain CD) male adult kidney, inner medulla cDNA
            to mRNA.
  ORGANISM  Rattus norvegicus
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Rodentia; Myomorpha; Muridae; Murinae.
REFERENCE   1  (bases 1 to 285)
  AUTHORS   Yu,A.S., Hebert,S.C., Brenner,B.M. and Lytton,J.
  TITLE     Molecular characterization and nephron distribution of a family of
            transcripts encoding the pore-forming subunit of Ca2+ channels in
            the kidney
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. (1992) In press
  STANDARD  full staff_entry
FEATURES             Location/Qualifiers
     CDS             <1..>285
                     /note="'PCR amplified fragment corresponding to skeletal
                     muscle L-type calcium channel'"
                     /product="calcium channel alpha-1 subunit"
                     /codon_start=1
                     /translation="PQAVLLLFRCATGEAWQEILLACSYGKRCDPESDYAPGEEYACG
                     TNFAYYYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKA"
BASE COUNT       56 a     87 c     76 g     66 t
ORIGIN
        1 ccacaagctg tgctgctgct cttcaggtgt gccacagggg aagcctggca ggagatcctg
       61 ctggcctgca gctacgggaa acgctgcgac ccggagtctg actacgcacc gggggaggag
      121 tacgcgtgtg gcaccaactt tgcctactac tacttcatca gcttctacat gctctgcgcc
      181 ttcctgatca tcaacctctt tgtggctgtc atcatggaca actttgacta cctcacaagg
      241 gactggtcca ttctggggcc tcaccacctg gatgagttca aggcc
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human chromosome 4 sequence-tagged site STS4-138
Date: 21 Aug 92 10:06:53 GMT
Distribution: bionet
Checksum: 19313     3

LOCUS       HUM4STS138    389 bp ds-DNA             PRI       21-AUG-1992
DEFINITION  Human chromosome 4 sequence-tagged site STS4-138
ACCESSION   M95228
KEYWORDS    STS; human chromosome 4; sequence tagged site.
SOURCE      Homo sapiens, clone C4-51 from Los Alamos National Laboratory
            chromosome 4 cosmid library, plate 4-1-180R, pos 1B; vector
            SuperCos 1 (Stratagene)
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 389)
  AUTHORS   Goold,R.D., diSibio,G., Dugaiczyk,A., Smith,K.A., Xu,H.,
            Magrane,G., Lang,D.B., Cox,D.R., Masters,S.B. and Myers,R.M.
  TITLE     Sequence-Tagged Sites for Human Chromosome 4
  JOURNAL   Unpublished (1992) see COMMENT for author address
  STANDARD  full automatic
COMMENT     PCR components: 25 ng of human genomic DNA, 10 pmol of each
            oligonucleotide, 200 micro-M dNTPs, 0.25 U Taq  polymerase (Cetus)
            in 10 micro-l of 50 mM KCl-20 mM  Tris-HCl, pH 8.3 (at room temp),
            2.5 mM MgCl-2.  Initial denaturation at 94degC for 1.5 min, then 30
            cycles of  94degC for 15 sec, 62degC for 23 sec, and 72degC for 30
            sec, followed by a final extension at 72degC for 3.5 min, using a
            Perkin-Elmer 9600 thermocycler. PCR-amplified product size 131 bp.
            Sequence submitted by:
            Human Genome Mapping Center
            Box 0925
            University of California San Francisco
            San Francisco, CA 94143-0925  USA
            Phone: (415) 502-1612   Fax: (415) 476-8391
            e-mail: hgmcprobes@cgl.ucsf.edu
FEATURES             Location/Qualifiers
     misc_feature    83..106
                     /label=Primer1
     misc_feature    complement(190..213)
                     /label=Primer2
BASE COUNT       99 a     76 c     57 g    131 t     26 others
ORIGIN
        1 atnaccagng cctgctgcat caactttttt tctatctctt tctttctttt nttnttttnn
       61 nngggtgaag tttaatgccg tnatcttgct tttctcccac cattgtatgt ttagaaanat
      121 ctatagagca gaatagtcaa aagcagagat tcttatgtac aantatagag attgaagtcc
      181 aagctctacc acatattagt tgtgtgaact tggctgantt atccaacatt nngntgtctc
      241 actctcctct cctcacaaaa gagataanat attgtggatt cagaacacag tggcccaggt
      301 acaccatgtt tttgttcctt ctnttgagga atgacataat tnnggnttcc aaatctaaan
      361 atcactnctt gnttattnca nncacctca
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Lentinus kauffmanii ribosomal RNA (35LR6) large subunit, partial.
Date: 19 Aug 92 10:01:29 GMT
Distribution: bionet
Checksum: 22590     2

LOCUS       LENRRLSAD     246 bp ss-rRNA            RNA       19-AUG-1992
DEFINITION  Lentinus kauffmanii ribosomal RNA (35LR6) large subunit, partial.
ACCESSION   M98627
KEYWORDS    large subunit ribosomal RNA.
SOURCE      Lentinus kauffmannii (library: Duke culture collection 619) cDNA to
            rRNA.
  ORGANISM  Lentinus kauffmannii
            Eukaryota; Fungi; Eumycota; Basidiomycotina; Hymenomycetes;
            Agaricales; Tricholomataceae.
REFERENCE   1  (bases 1 to 246)
  AUTHORS   Hibbett,D.S. and Vilgalys,R.
  TITLE     Phylogenetic relationships of the basidiomycete genus Lentinus
            inferred from molecular and morphological characters
  JOURNAL   Unpublished (1992)
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     rRNA            <1..>246
                     /note="Approximately aligns to region between positions
                     845 to 1093in Saccharomyces cerevisiae large subunit
                     nuclear rRNA."
                     /product="large subunit ribosomal RNA"
                     /citation=[1]
BASE COUNT       68 a     51 c     63 g     64 t
ORIGIN
        1 ctctgggtgg aggctcgtag cgattctgac gtgcaaatcg atcgtcaaat ttgggtatag
       61 gggcgaaaga ctaatcgaac catctagtag ctggttcctg ccgaagtttc cctcaggata
      121 gcagaaactc gtatcagatt tatgtggtaa agcgaatgat tagaggcctt ggggtgaaac
      181 aaccttaacc tattctcaaa ctttaaatat gtaagaacga gccgtcactt gattggaccg
      241 ctcggc
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human chromosome 4 sequence-tagged site STS4-320
Date: 21 Aug 92 10:08:56 GMT
Distribution: bionet
Checksum: 45006     3

LOCUS       HUM4STS320    700 bp ds-DNA             PRI       21-AUG-1992
DEFINITION  Human chromosome 4 sequence-tagged site STS4-320
ACCESSION   L00795
KEYWORDS    STS; human chromosome 4; sequence tagged site.
SOURCE      Homo sapiens, clone p4-243 from a chromosome 4 plasmid library;
            vector pBluescript II KS + (Stratagene)
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 700)
  AUTHORS   Goold,R.D., diSibio,G., Dugaiczyk,A., Smith,K.A., Xu,H.,
            Magrane,G., Lang,D.B., Cox,D.R., Masters,S.B. and Myers,R.M.
  TITLE     Sequence-Tagged Sites for Human Chromosome 4
  JOURNAL   Unpublished (1992) see COMMENT for author address
  STANDARD  full automatic
COMMENT     PCR components: 25 ng of human genomic DNA, 10 pmol of each
            oligonucleotide, 200 micro-M dNTPs, 0.25 U Taq  polymerase (Cetus)
            in 10 micro-l of 50 mM KCl-20 mM  Tris-HCl, pH 8.3 (at room temp),
            2.5 mM MgCl-2.  Initial denaturation at 94degC for 1.5 min, then 30
            cycles of  94degC for 15 sec, 62degC for 23 sec, and 72degC for 30
            sec, followed by a final extension at 72degC for 3.5 min, using a
            Perkin-Elmer 9600 thermocycler. PCR-amplified product size 373 bp.
            STS sequence contains simple sequence tandem repeats (CA and AT).
            Sequence submitted by:
            Human Genome Mapping Center
            Box 0925
            University of California San Francisco
            San Francisco, CA 94143-0925  USA
            Phone: (415) 502-1612   Fax: (415) 476-8391
            e-mail: hgmcprobes@cgl.ucsf.edu
FEATURES             Location/Qualifiers
     misc_feature    216..239
                     /label=Primer1
     misc_feature    complement(566..588)
                     /label=Primer2
BASE COUNT      217 a    140 c    108 g    223 t     12 others
ORIGIN
        1 gatcgtgcca ctgcactcca gcctgggtga cagagcaaga ccctgtctca aaaaagaaaa
       61 aaaaaagagt aaactgaggc acgcctgctt caaaagtgtc tgtcatcaac atctttctct
      121 agctctgtgt cccattggta atatttaaat gttaatttng gntttttcgc catcttagaa
      181 gattaatgag gaaatgttgg cataatattt agcgtttctt ggaagaaagc ttctggtgaa
      241 tacaattatc gttgtgcatt ttcataataa tgaaatatca cactatttnt atattcttgt
      301 tctcttnaat taatctttcc agtcccatca caatcaactt attttctaaa nccacagact
      361 ttggngtagt taacctttct gttttctcct aagtttaaat acacacacac acacacacac
      421 acacacacac acacacacac atatatatat atatatatat atatatatat atatatnatt
      481 tcattgattc cagcctcaag tatttatcac ttttgctttt gttttccctg agggtcagag
      541 ctgtagggct gaaagatatt aaaccctgcg tcagagaaga gtggtatctc ccccggtgga
      601 cagatgacct tatcagattt gaatttaggg gnncnaanca agcaaccgta ggttaaaact
      661 ttaactacta ctacagaatt aatatctgtn cctcttagag
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human chromosome 4 sequence-tagged site STS4-403
Date: 21 Aug 92 10:09:55 GMT
Distribution: bionet
Checksum: 49916     3

LOCUS       HUM4STS403    477 bp ds-DNA             PRI       21-AUG-1992
DEFINITION  Human chromosome 4 sequence-tagged site STS4-403
ACCESSION   L00840
KEYWORDS    STS; human chromosome 4; sequence tagged site.
SOURCE      Homo sapiens, clone p4-414 from a chromosome 4 plasmid library;
            vector pBluescript II KS + (Stratagene)
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 477)
  AUTHORS   Goold,R.D., diSibio,G., Dugaiczyk,A., Smith,K.A., Xu,H.,
            Magrane,G., Lang,D.B., Cox,D.R., Masters,S.B. and Myers,R.M.
  TITLE     Sequence-Tagged Sites for Human Chromosome 4
  JOURNAL   Unpublished (1992) see COMMENT for author address
  STANDARD  full automatic
COMMENT     PCR components: 25 ng of human genomic DNA, 10 pmol of each
            oligonucleotide, 200 micro-M dNTPs, 0.25 U Taq  polymerase (Cetus)
            in 10 micro-l of 50 mM KCl-20 mM  Tris-HCl, pH 8.3 (at room temp),
            2.5 mM MgCl-2.  Initial denaturation at 94degC for 1.5 min, then 30
            cycles of  94degC for 15 sec, 62degC for 23 sec, and 72degC for 30
            sec, followed by a final extension at 72degC for 3.5 min, using a
            Perkin-Elmer 9600 thermocycler. PCR-amplified product size 216 bp.
            Sequence submitted by:
            Human Genome Mapping Center
            Box 0925
            University of California San Francisco
            San Francisco, CA 94143-0925  USA
            Phone: (415) 502-1612   Fax: (415) 476-8391
            e-mail: hgmcprobes@cgl.ucsf.edu
FEATURES             Location/Qualifiers
     misc_feature    23..48
                     /label=Primer1
     misc_feature    complement(214..238)
                     /label=Primer2
BASE COUNT      138 a    112 c     72 g    149 t      6 others
ORIGIN
        1 gatcctccaa ttgctgcttc tagagtattg ttttaacttg ctcattcaaa aatccctttt
       61 ctgtgagtcc cacggcatct ttgggtagcc attttctcag ataataagca aagcatagtg
      121 tgattaggag catgaactcc tgagctatat actcctaggt ttaaacctca gattctccac
      181 ttattaagtg tctggccttg gtaaattatt taacctttca gtctcaattt catcatctgt
      241 aaaatgaggg taattataat gcctgtttca cagattttct gtgaacatta aatgacttat
      301 cacatgtaac agaacagtac caagcatgta gtagacattt tgtaaattcc agctgttatt
      361 taacaggntg aactcaatag gtgcacttcc tcttcctcgg nccacacaca cacacacaca
      421 ggncacacac acacacactc acacgngtcc cttnaaaccc attatangat ccacttt
//