From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Proteus mirabilis (clone pflidc1c2) flagellar hook-associated
Date: 28 Dec 92 21:31:02 GMT
Distribution: bionet
Checksum: 11151 10

LOCUS       PRMFLI       3567 bp ds-DNA             BCT       01-DEC-1992
DEFINITION  Proteus mirabilis (clone pflidc1c2) flagellar hook-associated
            protein (fliD) gene, 5' end; flagellin protein (fliC1) gene,
            complete cds; flagellin protein (fliC2) gene, complete cds.
ACCESSION   L07270
KEYWORDS    flagellar hook-associated protein; flagellin; fliC gene;
            fliC2 gene; fliD gene; promoter; repeat region; terminator.
SOURCE      Proteus mirabilis (strain BB2000) DNA.
  ORGANISM  Proteus mirabilis
            Prokaryotae; Gram-negative facultatively anaerobic rods;
            Enterobacteriaceae.
REFERENCE   1  (bases 1 to 3567)
  AUTHORS   Belas,R. and Flaherty,D.
  TITLE     DNA sequence and function of two flagellin genes of Proteus
            mirabilis
  JOURNAL   Unpublished (1993)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     RBS             complement(623..627)
                     /standard_name="Shine-Dalgarno sequence"
                     /gene="fliD"
                     /citation=[1]
                     /note="putative"
     promoter        complement(659..685)
                     /function="flagellin-specific promoter"
                     /standard_name="sigma-28"
                     /citation=[1]
                     /note="taaa-N15-tccgataa; putative"
     repeat_region   800..824
                     /rpt_type=direct
                     /standard_name="tandem direct repeat"
                     /citation=[1]
                     /note="putative"
     promoter        898..924
                     /standard_name="sigma-28"
                     /gene="fliC1"
                     /citation=[1]
                     /note="taaa-N15-gccgataa; putative"
     RBS             989..993
                     /standard_name="Shine-Dalgarno sequence"
                     /gene="fliC1"
                     /citation=[1]
                     /note="putative"
     terminator      2123..2165
                     /gene="fliC1"
                     /citation=[1]
                     /note="putative"
     repeat_region   2202..2235
                     /rpt_type=direct
                     /standard_name="tandem direct repeat"
                     /citation=[1]
                     /note="putative"
     promoter        2304..2330
                     /standard_name="sigma-28"
                     /gene="fliC2"
                     /citation=[1]
                     /note="taaa-N15-gacgatag; putative"
     RBS             2399..2403
                     /standard_name="Shine-Dalgarno sequence"
                     /gene="fliC2"
                     /citation=[1]
                     /note="putative"
     CDS             complement(<1..615)
                     /function="hook-associated protein necessary for capping
                     flagellum"
                     /standard_name="HAP2"
                     /gene="fliD"
                     /citation=[1]
                     /note="N-terminal region of FliD protein (HAP2) homologue;
                     assignment based on homology to E. coli and S. typhimurium
                     fliD gene and proximity to fliC; putative"
                     /codon_start=1
                     /translation="MAGISALGVGAKMPLGETLMQLEAAENKRLEPLDKQMKSYDAQI
                     TAYGKIRSQLDKLQKASEELKKFDKIVATKVDDEFDAFKVTTDGKASIGNYTVSVTEL
                     AHAQTLKSKAVSDIKDPIGETLGEGKTRTLVITQKGEKEPLKIELTDSQTSIIELRDA
                     INKKEGNVSATIVKAQDGVNHLVLTSRKEGTDSQMEITVEGDSKL"
     CDS             1002..2099
                     /standard_name="FliC1 protein"
                     /gene="fliC1"
                     /citation=[1]
                     /note="putative"
                     /product="flagellin"
                     /codon_start=1
                     /translation="MAQVINTNYLSLVTQNNLNKSQGTLGSAIERLSSGLRINSAKDD
                     AAGQAIANRFTSNVNGLTQASRNANDGISIAQTTEGALNEINNNLQRIRELTVQAKNG
                     TNSNSDITSIQNEVKNVLDEINRISEQTQFNGVKVLSGEKSEMVIQVGTNDNETIKFN
                     LDKVDNDTLGVASDKLFDTKTEKKGVTAAGAGVTDAKKINAAATLDMMVSLVKEFNLD
                     GKPVTDKFIVTKGGKDYVATKSDFELDATGTKLGLKASATTEFKVDAGKDVKTLNVKD
                     DALATLDKAINTIDESRSKLGAIQNRFESTINNLNNTVNNLSASRSRILDADYATEVS
                     NMSRGQILQQAGTSVLAQANQVPQTVLSLLR"
     CDS             2411..3514
                     /standard_name="FliC2 protein"
                     /gene="fliC2"
                     /citation=[1]
                     /note="putative"
                     /product="flagellin"
                     /codon_start=1
                     /translation="MAQVINTNYLSLVTQNNLNRSQSALGNAIERLSSGMRINSAKDD
                     AAGQAIANRFTSNINGLTQASRNANDGISVSQTTEGALNEINNNLQRIRELTVQAKNG
                     TNSNSDINSIQNEVNQRLDEINRVSEQTQFNGVKVLSGEKSKMTIQVGTNDNEVIEFN
                     LDKIDNDTLGVASDKLFDAKTEKKGVTAAGDAIDANALGISGSKKYVTGISVKEYKVD
                     GKVSSDKVVLNDGSDDYIVSKSDFTLKSGTTTGEVEFTGSKTTKFTADAGKDVKVLNV
                     KDDALATLDNAISKVDESRSKLGAIQNRFQSTINNLNNTVNNLSASRSRILDADYATE
                     VSNMSKNQILQQAGTAVLAQANQVPQTVLSLLR"
BASE COUNT     1168 a    636 c    647 g   1116 t
ORIGIN      
        1 aagcttacta tcaccttcaa cagtgatttc catttgagaa tctgtacctt ctttacgaga
       61 agtcagaact aaatggttaa cgccatcttg ggcttttaca atagtggcag aaacgttgcc
      121 ttctttttta ttgatagcat cacgtaattc aataatagaa gtttggctgt ctgttaactc
      181 tatttttaaa ggctcttttt cacctttttg ggtgatcact aaagtccgtg ttttgccttc
      241 acctaatgtt tcaccaatag gatctttgat atcactgacg gcttttgatt tcagtgtttg
      301 agcatgggca agctctgtta cagagaccgt ataattacca atgcttgctt taccatcagt
      361 ggttacttta aaggcatcaa actcatcatc aactttggtg gcgacgattt tatcgaattt
      421 ttttaattct tcagatgctt tctgtaattt atctaattgg ctacgaattt ttccatatgc
      481 agtaatttgt gcatcgtagc ttttcatctg tttgtctaaa ggttcaaggc gtttattttc
      541 cgcagcctct aattgcatca atgtttcgcc aagcggcatc ttggcgccta ctcctagtgc
      601 actaatacca gccatactgg tctcctttct gtgttcgtaa aataatccct acacagcgtt
      661 atcggaagaa ttggcgaaaa gtttacgtct atttactatt tttgatgagg gaaacagacc
      721 tccatataga agccttttat tgtcattgaa tactaaagtc gaattttgca ttaagaaaaa
      781 tggttcatgg cgagaattga taaaaataat ataaaaaaat aataaattaa tttctttata
      841 tatcatttgg ttaatggaga ttctataaaa aatgacagaa aaatttgttg gaaaagttaa
      901 aggttgtatc tggggtgccg ataaacttgt tgccggtgat tgacaccgta aatgataggc
      961 aaacttttat tttatcgact tgattttaaa ggaatctagc tatggcacaa gtcattaata
     1021 ccaactatct atccctggta actcaaaaca acctgaacaa atctcaggga actttaggaa
     1081 gtgcaatcga gcgtctgtct tctggtctac gtatcaacag tgcaaaagac gatgcagcgg
     1141 gtcaagcaat tgctaaccgt ttcacttcaa acgtaaatgg tttaactcaa gcttcacgta
     1201 atgcgaacga tggtatctct atcgctcaaa caactgaagg tgcattgaac gaaatcaaca
     1261 ataacttaca acgtatccgt gagttaactg ttcaagcgaa aaacggtact aactctaatt
     1321 cagatatcac ttctatccaa aacgaagtga agaacgtttt agacgaaatc aaccgtattt
     1381 ctgaacaaac tcagtttaac ggcgttaaag tactgagcgg tgagaaatca gaaatggtta
     1441 tccaagttgg tactaacgat aatgaaacta tcaaatttaa cttagataaa gttgataacg
     1501 atacattagg tgttgctagc gataaactgt ttgataccaa aacagagaaa aaaggtgtta
     1561 cagcagcagg tgcgggtgtt actgatgcta aaaaaatcaa tgctgctgcg acactggata
     1621 tgatggtatc actggtaaaa gaatttaatc ttgatggtaa accagtaact gataaattta
     1681 ttgttactaa aggtggtaaa gactatgtag caactaaaag tgattttgaa ttagatgcta
     1741 caggtactaa acttggatta aaagcatctg ccactacaga atttaaagtt gatgcaggta
     1801 aagacgttaa aactttaaac gttaaagatg acgctttagc aactttagac aaagctatca
     1861 acaccattga tgaaagccgt tctaaattag gtgcgattca aaaccgtttt gaatctacta
     1921 tcaacaacct aaacaacact gttaacaact tatctgcttc acgtagccgt atcttagatg
     1981 ctgactatgc aacagaagtt tctaacatga gccgtggtca aatcttgcaa caagcgggta
     2041 cttcagtact tgctcaagca aaccaagtac cacaaactgt tctgtctctg ttacgttaat
     2101 tcgtaccctg acacaaaaag atacctttct atatcaaagg ccctcatctg agggtctttt
     2161 ttgttatttt attttatatt taaactcact ctgttttagt ttattttttt ctctccatct
     2221 atttttgttt tttctgctag tcattatctc ttataaatta attaattggc tttttttgct
     2281 attttcgcga cggcgaaaac acataaatac cttcgattta atgacgatag tgtttaggca
     2341 acgttattac aggagtgtaa taacaaacct attaattaaa agtttttaaa taaaattgaa
     2401 ggaatattgt atggcacaag ttattaatac taattatctg tctttagtca ctcaaaataa
     2461 tctaaatcgt tcacaaagcg cacttggtaa tgcaattgaa cgtttatctt caggtatgcg
     2521 tattaacagc gcgaaagacg atgcggcagg tcaagctatt gctaaccgtt tcacttcaaa
     2581 tataaacggt ttaactcaag catctcgtaa tgcgaatgat ggtatttcag tgtctcaaac
     2641 cactgaaggt gcattgaacg aaatcaacaa taacttacaa cgtattcgtg aattaaccgt
     2701 tcaagcgaaa aacgggacta actctaactc agatatcaac tctatccaaa acgaagttaa
     2761 tcaacgttta gatgaaatca atcgtgtttc agaacaaact cagtttaacg gtgtaaaagt
     2821 gttgagtggt gaaaagtcaa aaatgaccat tcaagtgggt actaatgaca atgaagtgat
     2881 tgaattcaat ttagataaaa ttgataacga cacattaggt gttgctagtg ataaactgtt
     2941 tgatgctaaa acagagaaaa aaggggttac agcagcaggt gatgctatcg atgcaaacgc
     3001 tctgggcatt tcgggcagta aaaaatatgt gactggtatt tcagtaaaag agtataaagt
     3061 agatggtaag gtttcttctg ataaagttgt cttaaatgat ggtagcgacg actacatagt
     3121 aagcaaatca gactttacat taaaatcagg tactactact ggtgaagttg agtttactgg
     3181 tagtaaaacc actaaattta cagcagatgc aggtaaagat gttaaagttt taaatgttaa
     3241 ggatgacgct ttagcaactt tagataacgc aattagtaag gttgatgaaa gccgctctaa
     3301 attaggtgca attcaaaacc gtttccaatc tactatcaat aacttaaaca acactgttaa
     3361 taacttatct gcttcacgta gccgtatctt agatgctgac tatgcgacag aagtgtccaa
     3421 tatgagtaaa aaccaaatct tgcaacaagc aggtactgct gtgcttgctc aagcaaacca
     3481 agtaccacaa actgttctgt ctctgttacg ttaatccttc attaaactca tcttcagatc
     3541 cgggcaacgt tgttgccatt gctgcag
//