From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Streptococcus salivarius phosphoenolpyruvate:sugar
Date: 7 Jan 93 05:54:07 GMT
Distribution: bionet
Checksum: 10751 6

LOCUS       STREIPEPA    2259 bp ds-DNA             BCT       06-JAN-1993
DEFINITION  Streptococcus salivarius phosphoenolpyruvate:sugar
            phosphotransferase system enzyme I (ptsI) gene, complete cds.
ACCESSION   M81756
KEYWORDS    cytoplasmic protein;
            phosphoenolpyruvate:sugar phosphotransferase system enzyme I;
            protein kinase; ptsI gene; sugar transport protein.
SOURCE      Streptococcus salivarius (library: ATCC 25975) DNA.
  ORGANISM  Streptococcus salivarius
            Prokaryotae; Firmicutes; Firmibacteria; Gram-positive cocci;
            Streptococcaceae.
REFERENCE   1  (bases 1 to 2259)
  AUTHORS   Gagnon,G., Vadeboncoeur,C., Levesque,R.C. and Frenette,M.
  TITLE     Cloning, sequencing and expression in Escherichia coli of the ptsI
            gene encoding the enzyme I of the phosphoenolpyruvate: Sugar
            phosphotransferase system from Streptococcus salivarius
  JOURNAL   Gene 121, 71-78 (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     -35_signal      22..27
                     /gene="ptsI"
     TATA_signal     47..52
                     /gene="ptsI"
     terminator      1817..1847
                     /gene="ptsI"
                     /note="putative"
     terminator      1996..2023
                     /gene="ptsI"
                     /note="putative"
     CDS             79..1812
                     /gene="ptsI"
                     /product="phosphoenolpyruvate:sugar phosphotransferase
                     system enzyme I"
                     /codon_start=1
                     /translation="MTEMLKGIAASDGVAVAKAYLLVQPDLSFETVTVEDTSAEEARL
                     DAALAASQDELSVIREKAVESLGEEAAAVFDAHLMVLADPEMTGQIKETIRAKQVNAE
                     AALTEVTDMFIAIFEGMEDNPYMQERAADIRDVTKRVLANLLGKKLPNPATINEESIV
                     VAHDLTPSDTAQLNKKYVKAFVTNIGGRTSHSAIMARTLEIAAVLGTNNITELVKDGD
                     ILAVSGITGEVVINPTEEQIAEFKAAGEAYAKQKAEWALLKDAQTVTADGKHFELAAN
                     IGTPKDVEGVNDNGAEAVGLYRTEFLYMDSQDFPTEDDQYEAYKAVLEGMNGKPVVVR
                     TMDIGGDKELPYFDLPKEMNPFLGYRALRISISETGNQMFRTQLRALLRASVHGKLRI
                     MFPMVALLTEFRTAKGILEEEKAKLVAEGVAVADDIEVGIMIEIPAAAMLADQFAKEV
                     DFFSIGTNDLIQYTMAADRMNEQVSYLYQPYNPSILRLINNVIKAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSVLRTRSLMKKLDTAKMEEYANRALTECSTMEEV
                     LELSKEYVNVD"
BASE COUNT      683 a    438 c    478 g    660 t
ORIGIN      
        1 ctgcagaagg tgctgatgct gatgacgcaa tcgtagcaat cgcagaaaca atgacaaaag
       61 aaggattggc ataagaatat gacagaaatg cttaaaggaa tcgcagcatc tgatggtgtt
      121 gcagttgcta aggcatatct gcttgttcaa ccggacttgt catttgaaac tgtaacagtt
      181 gaagatacaa gtgcagaaga ggctcgtcta gatgctgctc tagcggcgtc acaagacgag
      241 ctttctgtta tccgtgaaaa agcagttgaa agccttggtg aagaagcggc agccgttttt
      301 gatgctcact tgatggttct tgctgaccca gaaatgactg gtcaaattaa agaaactatc
      361 cgtgctaaac aagttaatgc tgaagctgct ttgacagaag taacagacat gtttatcgct
      421 atttttgaag gtatggaaga caacccatac atgcaagaac gtgcagcgga tattcgtgac
      481 gttacaaaac gtgtgcttgc taacttgctt ggtaaaaaat tgcctaaccc agcaacaatc
      541 aacgaagaat caatcgttgt tgctcatgac ttgacaccat ctgatacagc tcagttgaac
      601 aaaaagtacg taaaagcttt tgttactaat atcggtggac gtacaagtca ctctgcaatc
      661 atggctcgta cacttgaaat cgcagccgtt cttggtacaa ataacatcac agaacttgtc
      721 aaagatggcg acattttggc ggtttcaggt attactggtg aagttgttat caacccaact
      781 gaagagcaaa ttgctgagtt taaagcagct ggtgaagctt acgctaaaca aaaagctgaa
      841 tgggctctcc ttaaagatgc tcaaacagtt actgctgatg gaaaacattt cgaattggct
      901 gctaacatcg gtacacctaa agacgttgaa ggtgtaaatg ataacggtgc agaagcagtt
      961 ggtctttatc gtacggaatt cttgtatatg gattctcaag acttcccaac tgaagatgac
     1021 caatatgaag catacaaagc agttcttgaa ggtatgaacg gtaaacctgt tgtcgttcgt
     1081 acaatggata tcggtgggga taaagaactc ccttacttcg atcttcctaa agaaatgaac
     1141 ccattcttgg gttaccgtgc tttgcgtatt tctatctctg aaactggtaa ccaaatgttc
     1201 cgtacacaat tgcgtgcctt gcttcgtgca tctgttcacg gtaaattgcg tatcatgttc
     1261 ccaatggttg ctttgttgac tgagttccgt actgctaaag gtattcttga agaagaaaaa
     1321 gctaaattgg ttgctgaagg tgttgccgtt gcagacgata tcgaagtagg tatcatgatt
     1381 gaaatccctg ctgcagctat gcttgctgac caatttgcta aagaagttga tttcttctca
     1441 attggtacaa acgaccttat ccaatacact atggctgctg accgtatgaa cgaacaagtt
     1501 tcataccttt accaaccata taacccatca atccttcgtt tgattaacaa tgttatcaaa
     1561 gcggctcacg ctgaaggtaa atgggctggt atgtgtggtg aaatggccgg tgaccaaact
     1621 gctgttccac ttcttgtcgg aatgggcttg gatgagttct caatgtcagc gacatctgtt
     1681 cttcgtacac gtagcttgat gaagaaactt gacacagcta aaatggaaga atatgctaac
     1741 cgtgcgctta ctgaatgttc aacaatggaa gaagttcttg aactttcaaa agaatacgtt
     1801 aacgtagact aatcataaaa agtggaggcc taggctttca ctttttttat aactaaacag
     1861 agacaaaata attgactcat tcattggtta ggagtagaat acggctaacc taggttaata
     1921 attaaaacta ttttttgccc taattgtcaa gtcaagtgca acaaagatgt tcctctgatt
     1981 aagtgagtat ctttcaagct gtttgagtta gctcaaacag cttttttgcg gactgatagc
     2041 catgaattcg tctaggtctt tcattgatag cctttgtata gtcctctaaa agattctgat
     2101 ttagttcttt taacgaacac cccttcggaa tgaactctct tagtagtcca ttgaaattct
     2161 catttgtacc tcgttcataa gatgaatagg catgtgcaaa ataaacatct aatccctcta
     2221 tcttactcaa tgatgaaaat tcgtttccat tatctgcag
//