From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: P.lutherii chloroplast secA gene, orf93 and orf42
Date: 21 Dec 92 20:35:00 GMT
Distribution: bionet
Checksum: 44473 8

LOCUS       PVLCPPLSED   3678 bp ds-DNA             ORG       18-NOV-1992
DEFINITION  P.lutherii chloroplast secA gene, orf93 and orf42
ACCESSION   X65961
KEYWORDS    secA gene.
SOURCE      Chloroplast Pavlova lutherii DNA.
  ORGANISM  Chloroplast Pavlova lutherii
            Eukaryota; Chromophycota; Chrysophyceae; Chrysophycidae;
            Ochromonadales.
REFERENCE   1  (bases 1 to 3678)
  AUTHORS   Scaramuzzi,C.D., Hiller,R.G. and Stokes,H.W.
  TITLE     Identification of a chloroplast-encoded secA gene homologue in a
            chromophytic alga: possible role in chloroplast protein
            translocation
  JOURNAL   Curr. Genet. 22, 421-427 (1992)
  STANDARD  full automatic
COMMENT     *source: isolate=CSIRO Fisheries Collection, Hobart, Australia, No.
            CS-23;
               From EMBL    entry CHPLSECA;  dated 31-JUL-1992.
FEATURES             Location/Qualifiers
     CDS             complement(301..582)
                     /note="orf93"
                     /codon_start=1
                     /translation="MRPIHICLNVRFLFFRPIYACNIDFITLAFAVRKSLFDDPLNPL
                     AIFKTLFLSYSSYTTSFNSHESPCIKSFTNSTLKINIYKILVNLPYLQK"
     CDS             776..3451
                     /gene="secA"
                     /product="secretory protein fortransport of proteins
                     across membranes"
                     /codon_start=1
                     /translation="MLKDILRKTTQSDLYWYENIVKKINDLERVMKPLTNEELRAKTL
                     GFRKSIEDGQSIDNILPEAFGLVWEASLRILGLRHYDVQLIGGCILHDSKIAEMKTGE
                     GKTLVAILPAYLNALSGKSVHIVTVNEYLAKRDSLSVGRVLSFLGLSVGLILADMNRE
                     ERQENYKCDVIYTTNSELGFDYLRDNLVGNPSEKVQNGFEFAIIDEVDSVLIDEARTP
                     LIISRSLETLNNIYLTAKNVAQAFEINTHYEIDKRNRNVYLNESGSKLAEKLLGVSSI
                     YKFETGTYILNAIKAKEFYTKDKDYLVMRNQITIVDEFTGRILKGRRWGDGLHQAIEA
                     KEGVTVGSETMTMASITYQNFFLFYKKLSGMTGTALTEAKEFKKIYNLSVDCVPINKK
                     VNRIDKEDVVYKSLYAKWKAVLYESLSIHEQGRPLLIGTSNVKNSEIVSGLLKEYNIK
                     HSLLNAKPENAANESEIIAQAGRKGSVTIATNMAGRGTDILLGGNPDFLTKGELRYIF
                     RSIVLSLDDMTVPKNELINNLKYKYVISEKNRLDVEELIDKLKSAYTVPEKNRIGVEE
                     LIENIDESFQPVDKFEILIQKLYEKTKEWYVRECLAEKEEVIQLGGLHIIGTEKHDSR
                     RIDNQLRGRAGRQGDPGSSKFFLSFEDRLIEIFTTGGLKNMIKELDLEDDQPVEGKIV
                     SLSIESAQKRIEDKNYQVRKQLFNYDNVLNLQRKVIYDERDRFLSLTDFKGLILQYLE
                     KLVDDVVAEMERSENQEDKSRGIVLFCKKFICLPYSIDPELLSNLSKEEIKIFLNDQV
                     KISYELKEIELESLRVGLSQSLEYAFLLQSIDQVWKEQLTRMELLKESIGWRAYGQRD
                     PLLEYQKEAYRIFAIQTRKIRHSASHLIMCSTSFA"
     CDS             3503..3631
                     /product="orf42"
                     /codon_start=1
                     /translation="MQVEETLLKKKQTINANNILSFNRVAVAPYSYIALTPYGQKI"
BASE COUNT     1411 a    482 c    737 g   1048 t
ORIGIN      
        1 ctgcaggaaa atagtattga atcaaacaaa ttagatatag aaatggaatt aagctcgatc
       61 gaagctataa agaatgcagt agaatcaggc ttgcgccgcc tttgtttcaa tatctgcgat
      121 aagaaaagaa ttagagttag acactcttag acatctaaaa gtaaatgact taactgttaa
      181 aagacaacta tcaattatta taaacaaaga aagatataga tcaaaggcag cagaaatgtt
      241 catgaaagag atactctttt ctacaaaaga tgagaatgaa attaatgatt aataaaaagt
      301 ttacttttgt aagtagggta aattgacaag tattttgtaa atatttattt ttagagtact
      361 attagtaaaa gattttatac aaggactttc atgagagtta aaagaggtag tgtagctaga
      421 gtaagaaaga aaaagtgttt taaagatagc tagcgggttt agaggatcgt caaatagact
      481 ttttcgtaca gcaaacgcaa gagttatgaa atctatgtta catgcatata ttgggcgaaa
      541 aaataaaaag cgtacattta ggcagatatg gattggacgc atcaaagcat ttgcgaatca
      601 aaagcagaca tcgtacagta aatttatgat gaacttaaaa aagtccaata ttggtctaaa
      661 tagaaaaatg ctatcacatt aatagtttta gaaggtttag catttgtgga cttttaaata
      721 aaaattctat gtaaagttac ataaaatttt agtgaaatat ggcaaatata gttttatgtt
      781 aaaggacata cttagaaaaa caacacaaag tgatttatac cggtacgaga atatcgttaa
      841 aaaaataaac gatctcgaaa gggttatgaa acctttgacc aatgaagaac ttagagctaa
      901 aacattgggt tttagaaaat caatagagga cgggcaaagc attgacaata tacttccaga
      961 agcctttgga ttagtacggg aagcgtcttt aagaattcta gggcttagac attatgatgt
     1021 gcaattgatt ggtggttgta tactccacga tagtaaaatc gccgagatga agacaggcga
     1081 gggaaaaaca ctggttgcaa tactacctgc atatttaaat gcgctaagtg gaaaaagtgt
     1141 acacatagtt acagtgaatg agtatttagc taaacgtgat agtttatcgg taggcagagt
     1201 acttagtttt cttggtctta gtgttggctt gatcttagct gatatgaata gagaagagag
     1261 gcaagaaaac tacaaatgtg acgttatata tacaacaaat agtgagctag gttttgatta
     1321 tttgagggat aacctagttg gaaatccttc tgagaaagtt caaaatggtt ttgaatttgc
     1381 gataattgat gaggttgatt ctgttttaat tgacgaagcg cgaacaccac taataatctc
     1441 ccgatcacta gaaacattaa ataatattta cctaacggcg aaaaatgtag cgcaagcatt
     1501 tgaaataaat actcactacg aaatagataa aagaaaccgt aatgtttatt taaatgaatc
     1561 tggtagcaaa ctagctgaaa aattacttgg cgttagtagt atatataaat ttgagactgg
     1621 cacttatata ttaaatgcaa taaaagcaaa agaattctat acgaaagata aagattatct
     1681 tgtaatgaga aatcaaatta caattgtaga tgagttcacc gggagaattt tgaaaggtag
     1741 gcgctggggt gatggtctcc atcaagctat agaagctaag gaaggcgtga cggttggtag
     1801 cgaaaccatg actatggcct cgatcactta ccagaatttc tttttgtttt acaaaaagtt
     1861 atccggtatg actggtacag cattaacaga agcgaaggaa ttcaaaaaaa tatataatct
     1921 atctgtggac tgcgtaccaa taaataagaa agttaatcgt atagacaaag aggatgttgt
     1981 ttacaaatct ctatatgcta aatggaaagc tgtcctatat gaatccttaa gtatacatga
     2041 gcaaggtaga ccattactta taggtacaag taacgtcaaa aactcagaga tcgtttcagg
     2101 tttattgaag gaatacaata taaagcatag tttacttaat gcgaaaccgg aaaatgcagc
     2161 taatgaatct gaaataattg cccaagccgg tagaaaagga agcgttacaa ttgcaacaaa
     2221 tatggcgggg cgtggtactg atattttatt aggtggtaat ccagactttt tgaccaaggg
     2281 tgagttaaga tacatattta ggtctatagt cttatcctta gatgatatga cagtgcctaa
     2341 aaacgagtta attaataacc ttaaatataa atatgttatt tctgagaaga ataggttaga
     2401 tgtcgaagaa ttaattgata aacttaaatc ggcttatact gttcctgaga aaaataggat
     2461 aggtgtcgaa gaattaattg aaaatattga cgagtctttt caaccagtag ataaattcga
     2521 aattttaata caaaagcttt atgaaaagac aaaagagcgg tatgtcagag aatgtttagc
     2581 cgaaaaggaa gaggttattc agcttggcgg tttacacata ataggtacgg aaaaacacga
     2641 ctctagaaga atagacaacc agttgagagg aagagctggt agacaaggag accctggctc
     2701 ttcaaaattt ttcttaagct ttgaagaccg cttgatagaa atttttacta cgggtggttt
     2761 gaaaaatatg ataaaagaac tggatttaga ggatgaccaa ccggttgaag ggaaaatagt
     2821 aagcctaagc atagagtcag ctcaaaagag aatagaagac aaaaactatc aagtaagaaa
     2881 acaattattt aactacgata acgttttgaa tctacagcga aaggtaattt atgacgagag
     2941 agatagattt ttaagtctta cagattttaa aggtctaatt ctccagtatt tagaaaagtt
     3001 agtagatgat gtggtagcag aaatggaaag gtctgaaaac caagaagaca aaagtagagg
     3061 tattgtactt ttttgtaaaa aatttatatg tcttccgtac tcgatagatc cagagctgct
     3121 tagcaatttg tcaaaagaag agataaaaat atttttgaat gatcaagtaa aaataagcta
     3181 tgaattgaaa gaaattgaac tagaatcatt gcgtgttggc ttgtctcagt ctcttgagta
     3241 tgctttttta ctgcaatcta tagaccaagt ttggaaagaa cagcttacta ggatggaact
     3301 gctaaaagaa agtattggtt ggagagctta tggacaaaga gatcctttac tcgaatacca
     3361 aaaggaagct tatagaatat tcgcaataca aacaagaaaa ataagacata gtgcttctca
     3421 tttaattatg tgttctacct cttttgctta aaaaaagaag gggctgttta ggtttcgatc
     3481 ctaaaaaaaa ctaataagtg tgatgcaagt agaggaaacc ctcttaaaaa aaaagcaaac
     3541 aataaatgca aacaatattt tatcttttaa tcgagtagct gtagccccgt atagttatat
     3601 agcattaact ccttatggtc aaaaaatcta gaaagtttga tcgtaaagca aaccttagct
     3661 agaagcacac aagaataa
//