HDTenEightyP/NetNews huggingface dataset:
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human chromosome 4 sequence-tagged site STS4-450
Date: 21 Aug 92 10:10:34 GMT
Distribution: bionet
Checksum: 55043     3

LOCUS       HUM4STS450    469 bp ds-DNA             PRI       21-AUG-1992
DEFINITION  Human chromosome 4 sequence-tagged site STS4-450
ACCESSION   L00870
KEYWORDS    STS; human chromosome 4; sequence tagged site.
SOURCE      Homo sapiens, clone p4-433 from a chromosome 4 plasmid library;
            vector pBluescript II KS + (Stratagene)
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 469)
  AUTHORS   Goold,R.D., diSibio,G., Dugaiczyk,A., Smith,K.A., Xu,H.,
            Magrane,G., Lang,D.B., Cox,D.R., Masters,S.B. and Myers,R.M.
  TITLE     Sequence-Tagged Sites for Human Chromosome 4
  JOURNAL   Unpublished (1992) see COMMENT for author address
  STANDARD  full automatic
COMMENT     PCR components: 25 ng of human genomic DNA, 10 pmol of each
            oligonucleotide, 200 micro-M dNTPs, 0.25 U Taq  polymerase (Cetus)
            in 10 micro-l of 50 mM KCl-20 mM  Tris-HCl, pH 8.3 (at room temp),
            2.5 mM MgCl-2.  Initial denaturation at 94degC for 1.5 min, then 30
            cycles of  94degC for 15 sec, 62degC for 23 sec, and 72degC for 30
            sec, followed by a final extension at 72degC for 3.5 min, using a
            Perkin-Elmer 9600 thermocycler. PCR-amplified product size 167 bp.
            STS sequence contains a simple sequence tandem repeat (CA).
            Sequence submitted by:
            Human Genome Mapping Center
            Box 0925
            University of California San Francisco
            San Francisco, CA 94143-0925  USA
            Phone: (415) 502-1612   Fax: (415) 476-8391
            e-mail: hgmcprobes@cgl.ucsf.edu
FEATURES             Location/Qualifiers
     misc_feature    72..96
                     /label=Primer1
     misc_feature    complement(213..238)
                     /label=Primer2
BASE COUNT      110 a     87 c     85 g    164 t     23 others
ORIGIN
        1 cntctnnctg gtgttncctt tnncaaatag gttcacgttt aaataaatta ctccatttat
       61 cggaaataaa tnccacagtt gtncaaatga ttgactgtgt tttcagagag ctcaaacatt
      121 tctncatctt gtttccctct ngggaagcca attcatttgt gtgtgtgtgt gtgtgtgtgt
      181 gtgtgtgtgt gtgtgtgtgt gtgaaatctc cccattctat taaatcaaac tgagcctgng
      241 acatactcta agagacaatn agagtatttt aacttttcta ancccatctn naaaaacatg
      301 tgtgtgngag tatatcatta tttcttncac atatgtgtct agtggggnct ntaagctccc
      361 tgncantctc ctaaggaaat gtggggtctg ccaacaggtc agttagtatt cctgacgtca
      421 ttttctttct cttccccatt actcttattg agaacgnaaa tantctttc
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Gnetum montanum copia-like retrotransposon reverse transcriptase
Date: 14 Aug 92 10:00:30 GMT
Distribution: bionet
Checksum: 39342     2

LOCUS       GNGCOPIA      272 bp ds-DNA             PLN       14-AUG-1992
DEFINITION  Gnetum montanum copia-like retrotransposon reverse transcriptase
            gene, partial cds.
ACCESSION   M94476
KEYWORDS    copia-like element; retrotransposon; reverse transcriptase.
SOURCE      Gnetum montanum DNA.
  ORGANISM  Gnetum montanum
            Eukaryota; Plantae; Embryobionta; Pinophyta; Gneticae; Gnetidae;
            Gnetaceae.
REFERENCE   1  (bases 1 to 272)
  AUTHORS   Voytas,D.F., Cummings,M.P., Konieczny,A., Ausubel,F.M. and
            Rodermel,S.R.
  TITLE     Copia-like retrotransposons are ubiquitous among plants
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 89, 7124-7128 (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     CDS             4..267
                     /note="PCR product"
                     /product="reverse transcriptase"
                     /codon_start=1
                     /translation="KTAFLHGDLEEEIYMLQPEGFAEEGKENLVCRLNKSLYGLKQAP
                     RCWYKRFDSYIMSLGYNRLNADPYTYFKRFDEDFIILLLYVDDM"
                     /partial
BASE COUNT       88 a     46 c     59 g     79 t
ORIGIN
        1 gttaagacag ctttcctgca tggagatctt gaagaagaga tttatatgct ccaaccagaa
       61 ggttttgcag aagaaggcaa agagaactta gtttgcagat tgaacaaatc tctgtacggt
      121 ctcaaacagg caccaaggtg ttggtacaaa agatttgact catatatcat gagccttggg
      181 tacaatagac tcaatgcaga cccttataca tatttcaaga ggtttgatga agatttcatc
      241 attttgctgt tatatgtaga tgacatggat cc
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human immunodeficiency virus type 2 (22ENVBB1) envelope (gp 41)
Date: 18 Aug 92 10:01:06 GMT
Distribution: bionet
Checksum: 31634     2

LOCUS       HIV2ENVB20    453 bp ss-RNA             VRL       18-AUG-1992
DEFINITION  Human immunodeficiency virus type 2 (22ENVBB1) envelope (gp 41)
            fragment.
ACCESSION   M87123
KEYWORDS    envelope protein; glycoprotein.
SOURCE      Human immunodeficiency virus type 2 uncultured blood mononuclear
            cells (5/89 sample) RNA.
  ORGANISM  Human immunodeficiency virus type 2
            Viridae; ss-RNA enveloped viruses; Positive strand RNA virus;
            Retroviridae; Lentivirinae.
REFERENCE   1  (bases 1 to 453)
  AUTHORS   Gao,F., Yue,L., White,A.T., Pappas,P.G., Barchue,J., Hanson,A.P.,
            Greene,B.M., Sharp,P.M., Shaw,G.M. and Hahn,B.H.
  TITLE     Human infection by genetically-diverse SIVsm-related HIV-2 in West
            Africa
  JOURNAL   Nature (1992) In press
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     misc_feature    1..453
                     /note="PCR derived HIV-2 envelope (gp 41) fragment"
                     /note="putative"
                     /citation=[1]
BASE COUNT      153 a     75 c    113 g    112 t
ORIGIN
        1 gacgtggtca aaagacaaca ggaaatgttg cggctgaccg tctggggaac taaaaacctc
       61 cagactagag tcactgccat cgagaagtac ctaaaggacc aagcaaaact aaattcatgg
      121 ggatgtgcct ttagacaggt ttgccacact actgtgccat gggtggacga cagtatgcag
      181 ccagagtggc aaaacatgac ttggcaacaa tgggaaaaac agattgcctt cttggaggat
      241 aatatcacag agctgttaga gcaagctcaa atacagcagg agaaaaatat gtatgagttg
      301 cagaaattaa atagctggga tgtttttggc aattggcttg atctcacctc ttgggtaaaa
      361 tatatctatt taggatttta tatagtagca ggagtaatag tgttaagaat agctatctat
      421 gtagtacaaa tgttaatgag acttaggaag ggc
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Lentinellus omphalodes ribosomal RNA (1LR0R) large subunit,
Date: 19 Aug 92 10:02:35 GMT
Distribution: bionet
Checksum: 60501     2

LOCUS       LNTRRLSA      237 bp ss-rRNA            RNA       19-AUG-1992
DEFINITION  Lentinellus omphalodes ribosomal RNA (1LR0R) large subunit,
            partial.
ACCESSION   M98638
KEYWORDS    large subunit ribosomal RNA.
SOURCE      Lentinellus omphalodes (library: Duke Herbarium DSH89-9) cDNA to
            rRNA.
  ORGANISM  Lentinellus omphalodes
            Eukaryota; Fungi; Eumycota; Basidiomycotina; Hymenomycetes;
            Agaricales; Tricholomataceae.
REFERENCE   1  (bases 1 to 237)
  AUTHORS   Hibbett,D.S. and Vilgalys,R.
  TITLE     Phylogenetic relationships of the basidiomycete genus Lentinus
            inferred from molecular and morphological characters
  JOURNAL   Unpublished (1992)
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     rRNA            <1..>237
                     /note="Approximately aligns to region between positions 70
                     to 306in Saccharomyces cerevisiae large subunit nuclear
                     rRNA."
                     /product="large subunit ribosomal RNA"
                     /citation=[1]
BASE COUNT       55 a     51 c     73 g     58 t
ORIGIN
        1 aaggattccc ctagtaactg cgagtgaagc gggaaaagct caaatttgta atctggcggc
       61 cttcggtcgt ccgagttgta atctagagaa gtgctttccg cgctggaccg tgtacaagtc
      121 tcctggaacg gagcgtcgta gagggtgaga atcccgtctt tgacacggac taccagtgct
      181 ctgtgatgcg ctctcgaagt cgagttgttt gggaatgcag ctcaaaatgg gaggtga
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: H.sapiens gene for CD59 protein, exon 2
Date: 22 Aug 92 10:00:47 GMT
Distribution: bionet
Checksum: 65215     3

LOCUS       HUMCD59EX2   1320 bp ds-DNA             PRI       22-AUG-1992
DEFINITION  H.sapiens gene for CD59 protein, exon 2
ACCESSION   Z14113
KEYWORDS    CD59 protein.
SOURCE      Homo sapiens DNA.
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 1320)
  AUTHORS   Tone,M.
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
REFERENCE   2  (sites)
  AUTHORS   Tone,M., Walsh,L.A. and Waldmann,H.
  TITLE     Gene structure of human CD59 and demonstration that discrete mRNAs
            are generated by alternative polyadenylation
  JOURNAL   J. Mol. Biol. (1992) In press
  STANDARD  full staff_review
COMMENT     *source: tissue=blood;
            From EMBL    entry HSCD59EX2;  dated 24-JUL-1992.
FEATURES             Location/Qualifiers
     intron          <1..654
                     /partial
     exon            655..739
     intron          740..>1320
                     /number=2
BASE COUNT      262 a    339 c    321 g    398 t
ORIGIN
        1 aagctttggc ttctttactg ggtaaagtag ggttggaggc atttcaggct ttccttcttc
       61 tcacctgatg ctatcactac acatctaggc attcacgtaa tggaaactta caaagttgct
      121 caatacctct cttcatgtcc ccaaagagag caagcaagtg tccccattat acttcttaca
      181 gtgaggagcc agagcttcca ggtatgctaa gccattgctc cctgttccac tgctgaatgc
      241 atgctggtgc cttccagagt cacccctttg gtctgattca cttagtgctg tggctccgag
      301 aagtggccct ctctttcagc cagcagcttg gggtggggcc agtagagtgg gaaccacagt
      361 ggctatatga gtcaatttgc tcatattgtc atttgactca ctgaccctga tggggaagat
      421 agtgttattc ccacagtgtg gtagtacaca ctggggctta tagggactga gcctactcaa
      481 gggtatatgg tgctgtgggt cagagctggg gcatggccca gggattcagt gtgccttgac
      541 tccccctgta aatgttcctc tcagaagcct tcttggcctt ccagcccttg gtttttgaga
      601 caaccagcag tcatttgttc gttcctgaca ttccttcctg tcccttcctt ccaggttctg
      661 tggacaatca caatgggaat ccaaggaggg tctgtcctgt tcgggctgct gctcgtcctg
      721 gctgtcttct gccattcagg tgagtgctcc agtctcagga catggatcta ggctgccttg
      781 gccatgaact cccttcttaa gcctcagttt cagccccagc tgctcctcca ggcctggctt
      841 tggttctttt gccttccagt gttacaggtc tggggttgta tgacagctcc ttcctatgtt
      901 atgaccttgt cctcagtggg gcatcgtcct gagttacagc ccgtcctgag ttacagcgtg
      961 gcttttggca ggggtgggtc aacaatcggc ggctgaagtt tcactcctgt caccataagc
     1021 tgcttgggga caccaatgga taccattctt agagtgctgt gcaagtccct ttggggtcca
     1081 ctctgcttag agccatctga gctcagtcct tctgctctga ggctcctcct gtgttttaat
     1141 gatgatgatg gtcatagtga cagcagcagc agctaactaa ccctgaagca tttatgtgcc
     1201 agtcactact ctaagcattt tacgtgtatt tgcttattta atcctcatgc caacctgatg
     1261 gagttggtgg tatttttatc cttgttttac aagcaacaag attgaggcct ccccaaaatt
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: A.eutrophus 16S rRNA
Date: 15 Aug 92 10:01:04 GMT
Distribution: bionet
Checksum: 19716     2

LOCUS       AFA16SRNA     158 bp ss-RNA             BCT       15-AUG-1992
DEFINITION  A.eutrophus 16S rRNA
ACCESSION   X59167
KEYWORDS    16S ribosomal RNA homologue; ribosomal RNA.
SOURCE      Alcaligenes eutrophus RNA.
  ORGANISM  Alcaligenes eutrophus
            Prokaryotae; Gracilicutes; Scotobacteria; Aerobic rods and cocci;
            Alcaligenaceae.
REFERENCE   1  (bases 1 to 158)
  AUTHORS   Busse,H.J., El Banna,T., Oyaizu,H. and Auling,G.
  TITLE     Identification of xenobiotic degrading isolates from the
            beta-subclass of Proteobacteria by a polyphasic approach including
            16S rRNA partial sequencing
  JOURNAL   Int. J. Syst. Bacteriol. 42, 19-26 (1992)
  STANDARD  full automatic
COMMENT     *source: strain=IAM 12368T;
            From EMBL    entry AE16SRNA;  dated 13-MAR-1992.
FEATURES             Location/Qualifiers
     rRNA            <1..>158
                     /note="16S rRNA"
BASE COUNT       41 a     38 c     48 g     31 t
ORIGIN
        1 gggcttcaca cgtcatacaa tggtgcgtac agagggttgc gaccgcgcga gggggagcta
       61 atcccagaaa acgcatcgta gtccggatcg tagtctgcaa ctcgactacg tgaagctgga
      121 atcgctagta atcgtggatc agcatgccac ggtgaata
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Liriodendron chinense copia-like retrotransposon reverse
Date: 14 Aug 92 10:00:55 GMT
Distribution: bionet
Checksum: 59700     2

LOCUS       LIRCOPIAA     281 bp ds-DNA             PLN       14-AUG-1992
DEFINITION  Liriodendron chinense copia-like retrotransposon reverse
            transcriptase gene, partial cds.
ACCESSION   M94477
KEYWORDS    copia-like element; retrotransposon; reverse transcriptase.
SOURCE      Liriodendron chinense DNA.
  ORGANISM  Liriodendron chinense
            Eukaryota; Plantae; Embryobionta; Magnoliophyta; Magnoliopsida;
            Magnoliidae; Magnoliales; Magnoliaceae.
REFERENCE   1  (bases 1 to 281)
  AUTHORS   Voytas,D.F., Cummings,M.P., Konieczny,A., Ausubel,F.M. and
            Rodermel,S.R.
  TITLE     Copia-like retrotransposons are ubiquitous among plants
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 89, 7124-7128 (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     CDS             13..276
                     /note="PCR product"
                     /product="reverse transcriptase"
                     /codon_start=1
                     /translation="KTAFPHGDIEEEIYMHQPIGYVAPGKENNVCRPKKSLYSLKQAP
                     RQWYKKFDSFMSENGYRRCHADHCCYLKKFDTSYIILLLYVDDV"
                     /partial
BASE COUNT       88 a     50 c     74 g     69 t
ORIGIN
        1 gaattcgatg ttaagacagc tttccctcat ggagacattg aagaagagat atatatgcat
       61 cagccgatag gatatgtggc accaggaaag gagaacaatg tgtgtagacc gaagaagagt
      121 ctatatagtc tgaagcaggc cccgaggcag tggtacaaga agttcgacag tttcatgtcg
      181 gaaaacgggt acaggagatg tcatgcagac cactgttgct acttgaagaa gtttgataca
      241 tcgtatatca ttcttctact gtatgtagac gacgtggatc c
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Gorilla gorilla MHC DQB1*06 gene, partial cds, clone GoDQB106.
Date: 12 Aug 92 10:00:39 GMT
Distribution: bionet
Checksum: 64786     2

LOCUS       GORMHDQBC     246 bp ds-DNA             PRI       12-AUG-1992
DEFINITION  Gorilla gorilla MHC DQB1*06 gene, partial cds, clone GoDQB106.
ACCESSION   M81266
KEYWORDS    major histocompatibility complex.
SOURCE      Gorilla gorilla (individual_isolate Rambo) blood DNA.
  ORGANISM  Gorilla gorilla
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Pongidae.
REFERENCE   1  (bases 1 to 246)
  AUTHORS   Bontrop,R.E., Jonker,M., van Weeren,P., Kenter,M. and Otting,N.
  TITLE     MHC-DQB repertoire variation in hominoid and old world primate
            species
  JOURNAL   J. Immunol. 149, 461-470 (1992)
  STANDARD  full staff_entry
FEATURES             Location/Qualifiers
     CDS             <1..>246
                     /gene="MHC DQ-beta 1"
                     /label=exon 2
                     /codon_start=1
                     /translation="YQFKGMCYFTNGTERVRLVSRNIYNREEIVRFDSDVGEFRAVTL
                     LGLPAAEYWNSQKDILERKRAAVDRVCRHNYQLELRTT"
BASE COUNT       58 a     63 c     85 g     40 t
ORIGIN
        1 taccagttta agggcatgtg ctacttcacc aacgggacag agcgcgtgcg tcttgtgagc
       61 agaaacatct ataaccgaga agagatcgtg cgcttcgaca gcgacgtggg ggagttccgg
      121 gcggtgacgc tgctggggct gcctgccgcc gagtactgga acagccagaa ggacatcctg
      181 gagaggaaac gggcggcggt ggacagggtg tgcagacaca actaccagtt ggagctccgc
      241 acgacc
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Rattus norvegicus 6-phosphofructo-2-kinase/fructose-2,
Date: 19 Aug 92 10:03:37 GMT
Distribution: bionet
Checksum: 23536     2

LOCUS       RAT6PKF       304 bp ds-DNA             ROD       19-AUG-1992
DEFINITION  Rattus norvegicus 6-phosphofructo-2-kinase/fructose-2,
            6-bisphosphatase gene, intron 1.
ACCESSION   M89910
KEYWORDS    6-phosphofructo-2-kinase; fructose-2, 6-bisphosphatase.
SOURCE      Rattus norvegicus (library: EMBL3) adult DNA.
  ORGANISM  Rattus norvegicus
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Rodentia; Myomorpha; Muridae; Murinae.
REFERENCE   1  (bases 1 to 304)
  AUTHORS   Lange,A.J., Espinet,C., Hall,R., El-Maghrabi,M.R., Vargas,A.M.,
            Miksicek,R.J., Granner,D.K. and Pilkis,S.J.
  TITLE     Regulation of gene expression of rat skeletal muscle/liver
            6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase: Isolation
            and characterization of a glucocorticoid response elements in the
            first intron of the gene
  JOURNAL   J. Biol. Chem. 267, 15673-15680 (1992)
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     protein_bind    168..182
                     /bound_moiety="glucocorticoid receptor"
     protein_bind    196..210
                     /bound_moiety="glucocorticoid receptor"
     protein_bind    229..242
                     /bound_moiety="NF-1"
BASE COUNT       73 a     51 c     50 g    130 t
ORIGIN
        1 actttagatt tagttcacta aaattttgtt ctgttgttga gtattttctt caggagactt
       61 aatattctca tgttgtcctt ctctggtttt gatgtcaagt tatactaaca tcatgcaatg
      121 aggtgagaac tattttcttt cattccatct ctgacaaagt ttgtcccaga actatctgtt
      181 cctcaaatat ttggcagaac tttcagatca aacagtctag tcctagtgtg gctttgaaac
      241 catttaactg ttacagtttc tctgaaagtt atggattttt tttgtttgtt tgtttgctac
      301 ttgt
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Babesia bovis aldo-keto reductase mRNA, 3'end.
Date: 18 Aug 92 10:00:26 GMT
Distribution: bionet
Checksum: 16040     3

LOCUS       BBOALDKET    1003 bp ss-mRNA            INV       18-AUG-1992
DEFINITION  Babesia bovis aldo-keto reductase mRNA, 3'end.
ACCESSION   M93122 M97631
KEYWORDS    aldo-keto reductase.
SOURCE      Babesia bovis (strain Samford attenuated) cDNA to mRNA.
  ORGANISM  Babesia bovis
            Eukaryota; Animalia; Protozoa; Microspora; Microsporea;
            Piroplasmia; Piroplasmida; Babesiidae.
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Dalrymple,B.P., Vuocolo,T. and Peters,J.M.
  TITLE     The sequence of a babesia bovis gene encoding a putative member of
            a new brance of the aldo-keto reductase superfamily
  JOURNAL   Unpublished (1992)
  STANDARD  full staff_entry
FEATURES             Location/Qualifiers
     CDS             1..859
                     /note="probable member of aldo-keto reductase superfamily"
                     /product="aldo-keto reductase"
                     /codon_start=2
                     /translation="SKVVIATKVASRSSHLSWLRSGDCKLSKDNILKAVDGSLSRLNT
                     DYIDLLQLHWPDRYVPMNANGDFHEVFHDTENMIDENSVPLEDQLDALQTLLTQGKIR
                     HWGLSNETPWGTLRFYKLAKQAGVAAPASVQLHYNLLCRNEVEKGFVELCRPQNTGIA
                     ILAYAPLAGGILTGKYLEYMDPTTSGRLLRFPSYMSRYRGSLAARAVKDYYNIAMSYK
                     YPNLCALALRWVLTRPFICSTVIGANDFYQLRENIHCSMPSLGITDLLEREINQLHWK
                     WRDPIRICQ"
                     /partial
BASE COUNT      277 a    202 c    223 g    301 t
ORIGIN
        1 gtcaaaggtt gttatcgcca caaaagttgc tagtaggagt tcacatttat catggctgag
       61 atcaggcgac tgtaagctat cgaaggataa tatattaaag gctgtcgacg ggtctttgtc
      121 tcgtttgaat acagactaca tcgatttatt gcaactacat tggcctgacc gttacgttcc
      181 aatgaatgct aacggggact ttcacgaagt cttccatgat actgagaaca tgatcgatga
      241 gaatagtgtc cctctagaag atcagttaga tgcacttcag acgttactaa cccagggaaa
      301 aattcgacat tggggtttgt ccaatgaaac tccctgggga acgttacgtt tctacaagct
      361 ggcaaaacaa gctggcgtag ctgctccagc atctgtgcaa ttgcattaca atttactttg
      421 ccgaaacgaa gtggaaaagg gatttgtgga attatgtcgt cctcaaaata caggcatcgc
      481 aattctggcc tacgcccccc ttgcgggtgg gatattgact gggaaatatt tggaatatat
      541 ggatccaacg acttcaggtc gcttactaag gttcccatca tatatgtcac gatacagggg
      601 ttcattggct gcacgtgcag tgaaggacta ttacaacatt gcaatgtcgt acaagtaccc
      661 taatttatgt gctcttgcct tacgctgggt gcttacgaga ccattcatct gtagtacagt
      721 aattggcgcg aacgactttt atcaattacg tgaaaacatt cactgctcaa tgccttcttt
      781 gggaatcact gacctactag agcgagagat taaccagctg cattggaagt ggcgtgatcc
      841 aattcgcatt tgtcagtagt atttttttct attggcaaat tccccctttg tttcaaacta
      901 tttgtagcag gaagtgtgct gatatcgcgc tattagttga gtttcgagtc atattaaact
      961 tcgttttaat tcgatgtgta atgttgcgac attttaaaaa aaa
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human chromosome 4 sequence-tagged site STS4-179
Date: 21 Aug 92 10:07:24 GMT
Distribution: bionet
Checksum: 32206     3

LOCUS       HUM4STS179    421 bp ds-DNA             PRI       21-AUG-1992
DEFINITION  Human chromosome 4 sequence-tagged site STS4-179
ACCESSION   M95262
KEYWORDS    STS; human chromosome 4; sequence tagged site.
SOURCE      Homo sapiens, clone C4-174 from Los Alamos National Laboratory
            chromosome 4 cosmid library, plate 4-1-2R, pos 12B; vector SuperCos
            1 (Stratagene)
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 421)
  AUTHORS   Goold,R.D., diSibio,G., Dugaiczyk,A., Smith,K.A., Xu,H.,
            Magrane,G., Lang,D.B., Cox,D.R., Masters,S.B. and Myers,R.M.
  TITLE     Sequence-Tagged Sites for Human Chromosome 4
  JOURNAL   Unpublished (1992) see COMMENT for author address
  STANDARD  full automatic
COMMENT     PCR components: 25 ng of human genomic DNA, 10 pmol of each
            oligonucleotide, 200 micro-M dNTPs, 0.25 U Taq  polymerase (Cetus)
            in 10 micro-l of 50 mM KCl-20 mM  Tris-HCl, pH 8.3 (at room temp),
            2.5 mM MgCl-2.  Initial denaturation at 94degC for 1.5 min, then 30
            cycles of  94degC for 15 sec, 62degC for 23 sec, and 72degC for 30
            sec, followed by a final extension at 72degC for 3.5 min, using a
            Perkin-Elmer 9600 thermocycler. PCR-amplified product size 213 bp.
            Sequence submitted by:
            Human Genome Mapping Center
            Box 0925
            University of California San Francisco
            San Francisco, CA 94143-0925  USA
            Phone: (415) 502-1612   Fax: (415) 476-8391
            e-mail: hgmcprobes@cgl.ucsf.edu
FEATURES             Location/Qualifiers
     misc_feature    64..86
                     /label=Primer1
     misc_feature    complement(252..276)
                     /label=Primer2
BASE COUNT      121 a     82 c     84 g    119 t     15 others
ORIGIN
        1 gaangtcctc catggnnagg ngactnaacc cccagtccag tgctatacaa gcaatagttg
       61 tncaatacat gctgcctcct gctaacagga gggnggaaga ctgctcaccc agtggtgact
      121 ggnggtttta ctaataaacg cattgccatg gtctcaagct ataattagag caagctccac
      181 aaaccaattg tcatcactca gaatggctca ccttcctgga ttataaaagt ttctagaatg
      241 taaatatttg tgtttgtatc gaaagatgac atatgcaaat gaaaacaatt tgtgttctac
      301 tctggtaaaa gttaggtccc ttagtagtag naaaaaaggt ttntagtggg ntttnccaaa
      361 tcttccntta ggnggtgctt tcaatggtaa cacttctntc aaacccagtt ttgttacaat
      421 g
//