From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Schizosaccharomyces pombe DNA polymerase delta (pold) gene,
Date: 29 Dec 92 20:00:42 GMT
Distribution: bionet
Checksum: 29866 9

LOCUS       YSPDNAPOLD   3751 bp ds-DNA             PLN       10-DEC-1992
DEFINITION  Schizosaccharomyces pombe DNA polymerase delta (pold) gene,
            complete cds.
ACCESSION   L07734
KEYWORDS    DNA polymerase; DNA polymerase-delta; promoter.
SOURCE      Schizosaccharomyces pombe (strain 972 h-) DNA.
  ORGANISM  Schizosaccharomyces pombe
            Eukaryota; Fungi; Eumycota; Hemiascomycetes; Endomycetales;
            Saccharomycetaceae.
REFERENCE   1  (sites)
  AUTHORS   Pignede,G., Bouvier,D.P., Recondo,A.M. and Baldacci,G.
  TITLE     Characterization of the POL3 gene product from Schizosaccharomyces
            pombe indicates inter-species conservation of the Catalytic subunit
            of DNA Polymerase Delta
  JOURNAL   J. Mol. Biol. 222, 209-218 (1991)
  STANDARD  full automatic
REFERENCE   2  (bases 1 to 3751)
  AUTHORS   Park,H., Francesconi,S. and Wang,T.S.-F.
  TITLE     Cell cycle expression of two respirative DNA polymerase alpha and
            delta from S. pombe
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     promoter        1..340
                     /gene="pold"
                     /evidence="EXPERIMENTAL"
     conflict        102
                     /gene="pold"
                     /citation=[1]
     exon            341..569
                     /number=1
                     /gene="pold"
                     /evidence="EXPERIMENTAL"
     conflict        419
                     /gene="pold"
                     /citation=[1]
     intron          570..621
                     /number=1
                     /gene="pold"
                     /evidence="EXPERIMENTAL"
     exon            622..3653
                     /number=2
                     /gene="pold"
                     /note="putative"
     conflict        777..784
                     /gene="pold"
                     /citation=[1]
     CDS             join(341..569,622..3653)
                     /gene="pold"
                     /note="putative"
                     /product="DNA polymerase delta"
                     /codon_start=1
                     /translation="MTDRSSNEGVVLNKENYPFPRRNGSIHGEITDVKRRRLSERNGY
                     GDKKGSSSKEKTSSFEDELAEYASQLDQDEIKSSKDQQWQRPALPAINPEKDDIYFQQ
                     IDSEEFTEGSVPSIRLFGVTDNGNSILVHVVGFLPYFYVKAPVGFRPEMLERFTQDLD
                     ATCNGGVIDHCIIEMKENLYGFQGNEKSPFIKIFTTNPRILSRARNVFERGEFNFEEL
                     FPVGVGVTTFESNTQYLLRFMIDCDVVGMNWIHLPASKYQFRYQNRVSNCQIEAWINY
                     KDLISLPAEGQWSKMAPLRIMSFDIECAGRKGVFPDPSIDPVIQIASIVTQYGDSTPF
                     VRNVFCVDTCSQIVGTQVYEFQNQAEMLSSWSKFVRDVDPDVLIGYNICNFDIPYLLD
                     RAKSLRIHNFPLLGRIHNFFSVAKETTFSSKAYGTRESKTTSIPGRLQLDMLQVMQRD
                     FKLRSYSLNAVCSQFLGEQKEDVHYSIITDLQNGTADSRRRLAIYCLKDAYLPQRLMD
                     KLMCFVNYTEMARVTGVPFNFLLARGQQIKVISQLFCKALQHDLVVPNIRVNGTDEQY
                     EGATVIEPIKGYYDTPIATLDFSSLYPSIMQAHNLCYTTLLDSNTAELLKLKQDVDYS
                     VTPNGDYFVKPHVRKGLLPIILADLLNARKKAKADLKKETDPFKKAVLDGRQLALKVS
                     ANSVYGFTGATNGRLPCLAISSSVTSYGRQMIEKTKDVVEKRYRIENGYSHDAVVIYG
                     DTDSVMVKFGVKTLPEAMKLGEEAANYVSDQFPNPIKLEFEKVYFPYLLISKKRYAGL
                     FWTRTDTYDKMDSKGIETVRRDNCPLVSYVIDTALRKMLIDQDVEGAQLFTKKVISDL
                     LQNKIDMSQLVITKALSKTDYAAKMAHVELAERMRKRDAGSAPAIGDRVAYVIIKGAQ
                     GDQFYMRSEDPIYVLENNIPIDAKYYLENQLSKPLLRIFEPILGEKASSLLHGDHTRT
                     ISMAAPSVGGIMKFAVKVETCLGCKAPIKKGKTALCENCLNRSAELYQRQVAQVNDLE
                     VRFARLWTQCQRCQGSMHQDVICTSRDCPIFYMRIAEHKKLQQSVDLLKRFDEMSW"
BASE COUNT     1173 a    657 c    727 g   1194 t
ORIGIN      
        1 ttttgttatg cccaagaata cattaagtag tggtttatgt agcttgatgg agattggtat
       61 aaacttgtat atttgatttc tttctccatc gtaatttgac agctaaccta tttaatgtgg
      121 ttttaccttt tcagcagttg acactcatac caaaatttat tggtgtacgc tttgaactta
      181 cctacaagat attcgcctaa gtacctattt gttgttacct acaaaaccct tttagttgct
      241 cgtcttccta ccaaaaacca agagcatgta agcttggtgt cttccttatt taccattttc
      301 tgttctaatt ttcataataa ataaaaaatt aattactaga atgacagata ggtcttcaaa
      361 tgagggcgtc gtgttaaata aagaaaacta tccatttccc cgaagaaatg gttcaattca
      421 tggcgaaatt acagatgtca aacggcgacg tttaagcgaa agaaatggat atggtgataa
      481 aaagggctca tcctcaaaag aaaaaacttc atcttttgag gatgagctgg ctgaatacgc
      541 ttcacaattg gaccaagatg aaataaaatg taaggttttt tggatagcta accatttatt
      601 agtatactga ctccttgata gcttcaaaag atcagcagtg gcaacgacca gctttacctg
      661 caataaatcc tgagaaggat gatatttatt ttcagcaaat cgatagtgag gagttcacag
      721 aaggatctgt accttcaatt cgtttgtttg gtgttactga caatggaaac agcatccttg
      781 ttcatgttgt gggattcctt ccgtactttt atgttaaagc cccggttgga tttcgacctg
      841 agatgctaga acgtttcaca caagatttag atgctacttg taatgggggt gtgatcgatc
      901 attgcattat agagatgaag gaaaatttat atggatttca gggaaatgaa aaatctccat
      961 tcattaaaat ctttactact aaccctcgta tattatctcg agcacgaaat gtatttgaaa
     1021 gaggagaatt caacttcgaa gagcttttcc ctgttggtgt cggtgtcact acttttgaga
     1081 gcaatacgca atatcttctt agattcatga ttgactgtga tgttgttggt atgaattgga
     1141 ttcatcttcc tgcttcaaaa tatcaatttc gttaccagaa tcgagtttct aattgtcaaa
     1201 ttgaagcttg gattaattac aaagacttga tcagtcttcc agctgaaggc cagtggtcta
     1261 aaatggcccc actgaggatc atgagctttg atattgaatg cgcaggtcgc aaaggcgttt
     1321 ttcctgatcc ctctattgac cctgtaattc aaattgcaag tatagtaacg cagtatggag
     1381 actccactcc ttttgttcgt aatgtctttt gtgttgatac atgctcacaa atcgttggaa
     1441 cacaagtata tgaatttcaa aatcaggctg aaatgctttc aagttggtca aaatttgttc
     1501 gtgatgttga cccagatgtt ttaattgggt acaacatctg caactttgat atcccttacc
     1561 tcttggatcg agcaaaaagc ttacgcattc acaatttccc attacttggc cgtattcata
     1621 attttttttc tgtcgcaaag gaaaccacgt tttcaagtaa agcatatggt actcgtgaaa
     1681 gcaaaaccac tagtattcct gggcgtttgc agcttgatat gttacaagta atgcagcgtg
     1741 attttaagct tcgatcttac tctttaaatg cagtctgctc tcagtttcta ggcgagcaaa
     1801 aagaagatgt acactattct atcatcactg acttgcaaaa tggcactgcc gattctagaa
     1861 gacgtttagc tatttattgt ctgaaagatg catatttacc ccaacgctta atggacaaat
     1921 taatgtgttt tgttaactat actgaaatgg caagagtaac aggtgtaccg tttaattttc
     1981 ttcttgctag aggccaacag attaaagtta tttctcaatt gttctgcaaa gctcttcaac
     2041 atgacctagt tgttccaaat atacgtgtaa atggaacaga tgagcaatat gaaggtgcta
     2101 cagtcattga accgattaaa ggatactatg atacccctat tgcaacactg gattttagtt
     2161 cactttatcc ttcgattatg caagcccata atttatgcta tacgacatta cttgactcaa
     2221 atactgcaga attattaaaa cttaagcaag atgttgacta ctcagtgact ccgaacggag
     2281 attatttcgt taaaccacat gttcgtaaag gactactccc tattatctta gctgatttac
     2341 ttaatgcacg taagaaagca aaagcagatc taaaaaagga aacggaccca ttcaaaaagg
     2401 ctgttttgga tggtagacag ctagctttaa aagtaagtgc taattcagta tatggattta
     2461 ctggtgctac caacggacgt ttgccgtgtt tagcaatttc ttcttctgtt acctcttatg
     2521 gtcgacaaat gattgaaaaa accaaggatg ttgtagaaaa gagatacaga attgagaatg
     2581 gatactctca cgatgcagtt gtaatctatg gtgacactga ttctgttatg gttaagtttg
     2641 gcgttaaaac gttacctgaa gccatgaaat taggagagga agcagctaat tatgtttcag
     2701 accagtttcc gaatcctatt aaactggagt ttgagaaagt gtactttcca tatcttttaa
     2761 tttccaaaaa acgttatgca ggattatttt ggactcgtac agatacttat gataaaatgg
     2821 actctaaagg aatagaaact gtcagaagag ataactgtcc tctagtatct tatgtgattg
     2881 acacagcttt gagaaaaatg ttaattgatc aggatgtaga gggtgcacag ttgtttacaa
     2941 aaaaagttat ttccgatttg ttacaaaaca aaattgatat gtcacaactt gtaatcacaa
     3001 aggcattatc aaaaactgat tatgctgcca aaatggcaca cgttgaattg gctgagcgaa
     3061 tgcgtaaacg agatgctggc tccgccccag ccataggaga tcgcgttgca tacgttatta
     3121 ttaaaggtgc acaaggtgat caattttata tgaggtcgga ggaccctata tatgtattgg
     3181 aaaataacat tcctattgat gcaaaatatt atttggaaaa tcaactctcc aaacctttgc
     3241 ttagaatttt tgagcctatt cttggtgaaa aagctagctc tttacttcat ggtgaccata
     3301 ctcggacgat ttccatggca gcccccagtg ttggaggaat aatgaaattc gcagtaaagg
     3361 ttgagacctg tcttggatgc aaagctccca taaaaaaagg taaaactgct ttatgtgaga
     3421 attgtctaaa tagatcggcg gaactgtatc aacgtcaggt tgctcaagtg aatgatttag
     3481 aagttcgttt tgctcgctta tggactcaat gtcagcgatg ccaaggaagt atgcatcaag
     3541 acgtcatttg taccagtaga gactgcccca tattttatat gcgaattgca gaacacaaga
     3601 aacttcagca atctgttgac ttactgaaaa gatttgatga aatgtcctgg tgaaaaagat
     3661 ggatgttttt aattactaaa tgtgtatatc aaacgtaatg atgaaaagtt ataataaaaa
     3721 ttctactttt cttaagtact tttgcaatgc g
//