HDTenEightyP/NetNews huggingface dataset:
From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: apolipoprotein C-I [rats, liver, mRNA Partial, 435 nt]
Date: 5 Nov 92 23:00:22 GMT
Distribution: bionet
Checksum: 43780 2

LOCUS       S110606       435 bp    mRNA            NLM       20-OCT-1992
DEFINITION  apolipoprotein C-I [rats, liver, mRNA Partial, 435 nt]
ACCESSION   S42394 X15512
KEYWORDS    .
SOURCE      rats liver
  ORGANISM  Unclassified.
            Unclassified.
REFERENCE   1  (bases 1 to 435)
  AUTHORS   Shen,P. and Howlett,G.J.
  TITLE     Two coding regions closely linked to the rat apolipoprotein E gene:
            nucleotide sequences of rat apolipoprotein C-I and ECL cDNA.
  JOURNAL   Arch. Biochem. Biophys. 297, 345-353 (1992)
  STANDARD  full automatic
COMMENT     This entry incorporates information provided by the National
            Library of Medicine's GenInfo Backbone as NCBI gibbsq 110606.
            This sequence comes from Fig. 2.
FEATURES             Location/Qualifiers
     CDS             84..350
                     /note="apo C-I;  For the protein sequence (NCBI gibbsq
                     110607):  This sequence comes from Fig. 3."
                     /product="apolipoprotein C-I"
                     /codon_start=1
                     /translation="MRLFIALPVLIVVVAMALEGPAPAQAAPDFSSAMESLPDKLKEF
                     GNTLEDKARAAIEHIKQKEIMIKTRNWFSETLNKMKEKLKTTFA"
BASE COUNT      114 a    114 c    119 g     88 t
ORIGIN      
        1 ggatcatagt ggtgggaggt ggtgggtact agtgatgccc cctatccgcg cgcgtctgcc
       61 tccagggtgc ccctccagcc aggatgaggc tcttcatcgc acttcctgtc ctgatcgtgg
      121 tcgtagccat ggctttggaa ggcccagccc ctgcccaggc ggcccctgac ttttccagcg
      181 caatggagag cttaccggat aaactaaaag agtttgggaa cactttggaa gataaggccc
      241 gggcagccat tgagcatatc aaacagaagg aaattatgat caagactcgg aactggtttt
      301 cagagacgct caacaaaatg aaggagaaat taaaaaccac atttgcctga gtgcctggcg
      361 agccacctcg cagcagcaaa gagggccacg tgacgggcca ggtccctctt caaataccaa
      421 taaaaacttg tagag
//
From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Brevibacterium sp. 23S rRNA gene insertion sequence.
Date: 6 Nov 92 18:14:52 GMT
Distribution: bionet
Checksum: 33369 2

LOCUS       BRL23RRNAA    104 bp ds-DNA             BCT       29-OCT-1992
DEFINITION  Brevibacterium sp. 23S rRNA gene insertion sequence.
ACCESSION   M85097
KEYWORDS    23S ribosomal RNA; insertion.
SOURCE      Brevibacterium sp. (library: DSM 20165) DNA.
  ORGANISM  Brevibacterium sp.
            Prokaryotae; Firmicutes; Irregular asporogenous rods.
REFERENCE   1  (bases 1 to 104)
  AUTHORS   Roller,C., Ludwig,W. and Schleifer,K.H.
  TITLE     Gram-positive bacteria with a high DNA G+C content are
            characterized by a common insertion within their 23S rRNA genes
  JOURNAL   J. Gen. Microbiol. 138, 1167-1175 (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     rRNA            1..104
                     /standard_name="23S rRNA insertion"
                     /note="this span absent in E. coli; located between
                     helices 54 and 55"
                     /product="23S ribosomal RNA"
BASE COUNT       19 a     29 c     34 g     22 t
ORIGIN      
        1 cgcccctggt gaatcagtga tgctaaccgt cctgaagccg gccggttcct tcgggaatcg
       61 gtcgtgtgga tgcacgggac ccgatctggt agtagccaag cgat
//
From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Leishmania amazonensis mini-exons.
Date: 6 Nov 92 18:07:48 GMT
Distribution: bionet
Checksum: 31689 2

LOCUS       LEIMINIEX     289 bp ds-DNA             INV       28-OCT-1992
DEFINITION  Leishmania amazonensis mini-exons.
ACCESSION   L05000
KEYWORDS    mini-exon.
SOURCE      Leishmania amazonensis (strain MHOM/Brazil/76/JOS) DNA.
  ORGANISM  Leishmania amazonensis
            Eukaryota; Animalia; Protozoa; Sarcomastigophora; Mastigophora;
            Kinetoplastida; Trypanosomatina; Trypanosomatidae.
REFERENCE   1  (bases 1 to 289)
  AUTHORS   Fernandes,O., Murthy,V.K., DeGrave,W.M. and Campbell,D.A.
  TITLE     Mini-exon variation in human pathogenic Leishmania species
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     exon            1..9
                     /number=1
                     /standard_name="mini-exon"
     intron          10..65
                     /number=1
     misc_feature    66..259
                     /note="intergenic region"
     exon            260..289
                     /standard_name="mini-exon"
BASE COUNT       36 a     89 c    104 g     60 t
ORIGIN      
        1 actttattgg tatgcgaaac ttccggaacc cgtcttccgg caagattttg gaagcgcgca
       61 cgcgattttt tttgtgtgcg cagcgccccg accaccattg cgcgggtgtg cgccgggcac
      121 ggggtgtgcg tgccggccgt tcccggcgac gcgcgctgcc ccgggccgtg gggccgaggg
      181 cgccggcagc cgtgacacgt ggcccggtgc ggtgcgggcg ggcgcccccc cggcgccgtg
      241 ctgtgggtga gcggctttta actaacgcta tataagtatc agtttctgt
//
From: POSTMASTER@NBRF.GEORGETOWN.EDU
Newsgroups: bionet.molbio.proteins
Subject: Temporary Interruption of BITNET Access to PIR
Date: 13 Nov 92 15:50:36 GMT
Distribution: bionet


            Announcement of Temporary Interruption of Access to
                     The Protein Information Resource

The National Biomedical Research Foundation will not have direct access to
BITNET from 17:00 EST Friday 20 November through about 12:00 EST Monday 23
November because of a system upgrade to be performed on a connecting node.
Users who normally access the PIR Network Request Server through BITNET
channels can expect a delay in response until full channel operation is
restored.

This interruption will not affect our On-line System users or users who
normally access the server through Internet channels.  BITNET users may
wish to attempt to access our server through a gateway at the Internet address
FILESERV@NBRF.GEORGETOWN.EDU

We regret any inconvenience this temporary loss of service will cause.
------------------------------------------------------------------------
                                 Dr. John S. Garavelli
                                 Database Coordinator
                                 Protein Information Resource
                                 National Biomedical Research Foundation
                                 Washington, DC  20007
                                 POSTMASTER@GUNBRF.BITNET
                                 POSTMASTER@NBRF.GEORGETOWN.EDU
From: bfrank@aardvark.ucs.uoknor.edu (FRANK,BART)
Newsgroups: bionet.molbio.proteins
Subject: anti-GST antibodies for fusion protein detection
Date: 12 Nov 92 21:14:00 GMT
Organization: University of Oklahoma - University Computing Services
Originator: usenet@midway.ecn.uoknor.edu
News-Software: VAX/VMS VNEWS 1.41

We are attempting to produce a GST fusion protein and either little
of it is made or little survives proteolysis.  We would like to 
demonstrate its presence by showing the expected increase in
molecular weight on a Western blot with an anti-GST antibody.
Can anyone recommend a source for such a reagent?

Thanks, 
Bart Frank
Internet: BFRANK@AARDVARK.UCS.UOKNOR.EDU
Newsgroups: bionet.molbio.proteins
From: duca@binah.cc.brandeis.edu
Subject: ...looking for just the right peptide...
Reply-To: duca@binah.cc.brandeis.edu
Organization: Brandeis University
Date: Sun, 8 Nov 1992 13:37:24 GMT

Hi there all you exquisitely architectured biological (and/or artificial)
        neural nets,

I'm trying to develop a theoretical model of ion channel formation within
a lipid bilayer.  I've settled on using certain "designer channels" made
by Lear and DeGrado at DuPont about 5-7 years ago, but unfortunately no
spectroscopy has been done on them and we don't have too clear an idea of
their structures.  Does anyone know of any serine and leucine rich alpha
helical proteins with good structural data available (X-ray or NMR)?
Seeing as initial parameter setting is so crucial in these models I want
to try to start with some known interatomic distances as first approximations.
Can anybody make a suggestion?  I'd really appreciate it.  Thanx in advance.

Karen Duca
Dept. of Biophysics
Brandeis U.
duca@binah.cc.brandeis.edu
Newsgroups: bionet.molbio.proteins
From: C-Y Khoo <cyk10@cus.cam.ac.uk>
Subject: anti-GST antibodies for fusion protein detection
X-Xxmessage-Id: <A7294C7D4C012D4A@cy-mac.welc.cam.ac.uk>
X-Xxdate: Fri, 13 Nov 92 12:13:49 GMT
Organization: Wellcome/CRC Institute, Cambridge
X-Useragent: Nuntius v1.1.1d11
Date: Fri, 13 Nov 1992 12:13:49 GMT

I too, have been looking for anti-GST antibodies, but without any success.
I called Pharmacia and they said that they are in the process of
developing
such a reagent, but that it won't be ready for some time yet. There are
commercial sources of anti-GST antibodies, but they're raised against the
human antigen, and according to Pharmacia, don't cross react with the S.
japonicum antigen. Your best bet is to express pGEX, purify GST from
the supernatant, and immunize a rabbit with it.

Depending on various things, I _may_ have some anti-GST by January next
year
(I'm currently immunizing with a fusion protein). Ask me later!

Regarding your expression problems, I found out that _most_ fusion
proteins
are insoluble and are found in inclusion bodies, _not_ the supernatant (as
stated in Smith and Johnson (or indeed, Current Protocols in Molecular
Biology). See Fiona Marston's articles in Biochem J. (1986) 240, 1-12 and
an article of the same name in another journal (don't have the reference
at hand, unfortunately!). Also, Nagai and Thogersen (1987) Methods Enzymol
153, 461-481 for inclusion body prep. As a first step, you may want to
boil some of the pellet to see if the fusion protein is actually being
produced, but in an insoluble form.

Good luck!

=======================================================================
=======
Chong-Yee Khoo                             E-mail: cyk10@cus.cam.ac.uk
Wellcome/CRC Institute                     Talk:  
cyk10@cy-mac.welc.cam.ac.uk
Tennis Court Road                          Phone:  +44 223 334110
Cambridge                                  Fax:    +44 223 334089
CB2 1QR
Newsgroups: bionet.molbio.gene-linkage
From: horn@schaefer.math.wisc.edu (Jeffrey Horn)
Newsgroups: bionet.molbio.gene-linkage
Subject: PD Genetic Mapping Software
Date: 10 Nov 92 21:59:21 GMT
Organization: Univ. of Wisconsin Dept. of Mathematics

Would anyone have any leads on acquiring public domain or at least
freely available genetic linkage software (for plant populations).
I am interested in calculating genetic maps, displaying them in 
MS-Windows or X Windows, and printing the maps.  I am aware of 
Lander's Mapmaker program, but would like something a bit more
automatic and a bit more graphical.

Thanks for your help, 

Jeffrey Horn
.
From: toby@carson.u.washington.edu (Toby Bradshaw)
Newsgroups: bionet.molbio.gene-linkage
Subject: Re: PD Genetic Mapping Software
Date: 13 Nov 92 18:36:06 GMT
Organization: University of Washington, Seattle

In article <1992Nov10.215921.10509@schaefer.math.wisc.edu> horn@schaefer.math.wisc.edu (Jeffrey Horn) writes:
>Would anyone have any leads on acquiring public domain or at least
>freely available genetic linkage software (for plant populations).
>I am interested in calculating genetic maps, displaying them in 
>MS-Windows or X Windows, and printing the maps.  I am aware of 
>Lander's Mapmaker program, but would like something a bit more
>automatic and a bit more graphical.

I saw Steve Lincoln at Plant Genome I, and MAPMAKER 3.0 has graphics
capability.  As for "automatic" -- it's automatic enough for most
folks, and it isn't always safe to trust the default settings anyway.

Toby Bradshaw
Department of Biochemistry and College of Forest Resources
University of Washington, Seattle
toby@u.washington.edu
From: basand@bb1t.monsanto.com
Newsgroups: bionet.molbio.genome-program
Subject: test message, ignore
Date: 10 Nov 92 16:26:04 GMT
Organization: Monsanto Company, St. Louis, MO

this is a test message, 

please read and discard at will.
From: kristoff@net.bio.net (David Kristofferson)
Newsgroups: bionet.jobs
Subject: Re: Career Opportunities at ...
Date: 5 Nov 92 06:47:00 GMT
Distribution: usa
Organization: BIOSCI International Newsgroups for Biology

I regret to have to remind everyone once again that NSF has not given
permission to post commercial job offerings here on bionet.jobs.  I am
continuing to make inquiries to get this policy changed but for now
please limit postings to jobs in the academic and non-profit sectors
in biology.

                                Sincerely,

                                Dave Kristofferson
                                BIOSCI/bionet Manager

                                kristoff@net.bio.net
From: figuei@caladan.rutgers.edu (Francisco Figueirido)
Newsgroups: bionet.jobs
Subject: Technical position available (protein folding)
Keywords: protein folding, NMR, CD
Date: 7 Nov 92 01:15:27 GMT
Organization: Rutgers Univ., New Brunswick, N.J.

I posted this a while ago but it seems it didn't get to the network, so I am 
reposting it.
===============================================================================

I am posting this for my wife. If you find it more convenient to reply by
e-mail please do so to me at the following address:

	figuei@lutece.rutgers.edu

===============================================================================

                            RESEARCH AIDE POSITION


Position            Research Aide (one year appointment, renewable).
Department          Chemistry (Biochemistry Division).
Responsibilities    Perform research (under supervision) on protein fold-
                    ing and carbohydrate recognition using recombinant
                    DNA, chemical, biochemical and biophysical techniques
                    (UV, Fl, CD, NMR spectroscopy). General lab duties of
                    ordering materials, mantaining instrumentation, and
                    keeping technical protocols.
Qualifications      A Bachelor's degree and experience in the field of
                    Biochemistry and Chemistry required.
Salary              Commensurate with experience.


Position available immediately. Please send resume including three ref-
erences to
                                Maria Luisa Tasayco
                                Assistant Professor of Chemistry
                                Chemistry Department
                                City College of New York
                                Convent Av. at 138 Street
                                New York, NY 10031
From: HSIEH@CVLAB.HARVARD.EDU (CHUNG-MING HSIEH)
Newsgroups: bionet.jobs
Subject: (none)
Date: 6 Nov 92 00:38:59 GMT
Distribution: bionet

Please reply this open position to the following email address:

LEE@CVLAB.HARVARD.EDU

or the address listed in the following message.  DO NOT REPLY TO THE ADDRESS
WHERE THE MESSAGE WAS SENT OUT!!!
____________________________________________________________________________
				POSITION OPEN			Science 10/9
____________________________________________________________________________

HARVARD/POSTDOCTORAL POSITIONS are available to study molecular mechanisms of 
atherosclerosis and hypertention with special emphasis on identification of 
cis- and trans-acting factors that regulate genes important for growth and 
differentiation of vascular endothelial and smooth muscle cells (J. Biol.
Chem. 266:19034, 266:16188, 267:9467).  Experience with molecular biology,
protein chemistry, cell biology, or transgenic animals is desirable.  Please
send curriculum vitae and names of three references to:

M.E. Lee, M.D., Ph.D.
Cardiovascular Biology Laboratory,
Building 2, Rm 129B
Harvard School of Public Health
665 Huntington Avenue
Boston, MA 02115 
(Fax) (617)432-0031
From: u235@rex.chb.uokhsc.edu (Yung-Jin Lee)
Newsgroups: bionet.jobs
Subject: Job wanted: for Vascular Pharmacologist
Date: 13 Nov 92 22:53:40 GMT
Followup-To: bionet.jobs
Organization: University of Oklahoma Health Sciences Center, OKC
Originator: usenet@essex.ecn.uoknor.edu


	This post is for one of my friends who doesn't
have e-mail address.  
------------------------------------------------------------------
I (Chia-Hsuh Hsu) am looking for a position that involves in the 
studies of vascular pharmacology.  Right now I'm doing the post-
doctoral research in this field.  Resume will be sent on request.
Please e-mail me.

Chia-Hsuh Hsu
------------------------------------------------------------------
From: utopia@nynexst.com (Sadhan Samal)
Newsgroups: bionet.jobs
Subject: Information Request for jobs in Middle East for a Medical Doctor
Date: 13 Nov 92 20:54:46 GMT
Organization: NYNEX Science & Technology, Inc.

I will appreciate any information regarding job oppurtunities in the
Middle East specifically in Saudi Arabia or Kuwait for a medical doctor
having 15+ years of experience.

Please send reply to my E-MAIL address:

		utopia@nynexst.com

Thanks in advance.

Sadhan
From: speedy@vax.oxford.ac.uk
Newsgroups: bionet.jobs
Subject: Visiting Research Fellowship
Date: 11 Nov 92 17:30:16 GMT
Organization: Oxford University VAX 6620

Herewith notice of a visiting fellowship that may be of interest
to scientists of various disciplines.  I am sending it to a number
of groups on NETNEWS.  Please bring it to the attention of any
suitable colleagues.  Replies to Dean's secretary at the address 
below - not by e-mail, please! Please indicate where you saw the
advert.

Andrew Speedy
Tutor in Biological Sciences
Christ Church
Oxford

---------------------------------------------------------------------
ADVERTISEMENT


CHRIST CHURCH,  Oxford



Dr Lee Visiting Research Fellowships in the Sciences


The college proposes to elect Visiting Fellows, in the Natural
Sciences and Mathematics, for up to nine months in the period
September 1993 to August 1994.

The Fellowships are intended to enable distinguished senior
scholars to pursue their own research as members of the college
community, and they will be expected to reside in Oxford during
the period of tenure.    Fellows must make their own research
arrangements with Oxford laboratories.

Each Fellow will be entitled to free family accommodation, a
study-room in college and free dinners.   Return fares from the
place of residence will be paid for each Fellow and his/her
family.   Limited stipends may also be offered, depending on
individual circumstances.

Further details may be obtained from The Dean's Secretary, Christ
Church, Oxford OX1 1DP, and applications should be received not
later than
12 December 1992.
From: BI599146@BROWNVM.BROWN.EDU (Paolo DePetrillo MD)
Newsgroups: bionet.jobs
Subject: Re: DNA Sequencing Technician Sought
Date: 9 Nov 92 17:11:42 GMT
Distribution: bionet

Master's level technical position open in the Division of Clinical Pharmacology
at Brown University, Providence, RI.  Earliest start date Dec. 1 1992.  We are
characterizing the acute and chronic effects of ethyl alcohol on protein kinase
C levels and activities in human peripheral circulating lymphocytes, both in
vitro and in vivo.  Familiarity with use of P(32) for enzymatic assays,
SDS/PAGE, Western and Northern blotting, and isolation of mRNA preferred, but
not absolutely required, depending on experience.  Position exists until 7/1/94
at which time it may be renewed.  Salary is commensurate with experience, and
includes an excellent benefits package including health and disability
insurance.  Reply to this message at BI599146@brownvm.brown.edu, or
FAX (USA) 401-456-6744 or send CV to:
P. B DePetrillo, MD
P.O. Box G/RWH
Brown University
Providence, RI 02912 (USA)
Newsgroups: bionet.jobs,bionet.research
From: ajsmith@nyx.cs.du.edu (Aaron Smith)
Subject: BIO Student Seeks Lab Position
Organization: Nyx, Public Access Unix @ U. of Denver Math/CS dept.
Distribution: na
Date: Fri, 6 Nov 92 16:08:09 GMT



Hello, I am a Biology Student at the College of William and Mary.  For the
summer of 1993 I wish to gain experience working in a lab.  I am not
particularly interested in any type of compensation, merely a place to 
stay, and the experience I can gain from working in the lab.  Please
contact me via e-mail if you have a position that I would qualify for,
or write me to obtain a listing of my qualifications and experience.
Thank You!

Phuong T. Lui
College of William and Mary
c/o Aaron J. Smith
    Virginia Tech

Newsgroups: bionet.jobs
From: jrichmn@ccu.umanitoba.ca (Joy Richman)
Subject: Developmental Biology studentship available
Organization: University of Manitoba, Winnipeg, Canada
Date: Wed, 11 Nov 1992 21:26:58 GMT

ATTENTION FINAL YEAR B.Sc. STUDENTS!!!

I have a position available in my lab (funded!!) for a M.Sc. student. I am
seeking a student interested in Developmental Biology with a B.Sc. degree
obtained in North America or Western Europe. 

The model system I am using is the chick embryo and I am focusing on
facial development. I have been combining both the traditional,
experimental embryo manipulation approach and the molecular approach to
elucidate the controls of facial patterning. The training I can offer in
my laboratory will prepare you for many acadmeic job opportunities. The
molecular biology of Development is fast becoming a key area of interest
being expanded in University departments around the world (see the
job advertizements in Nature and Cell). My laboratory currently
enjoys funding from the Medical Research Council of Canada and the
National Institute of Dental Research in the U.S.A. and can offer a
student many interesting projects. 

The position is available from January 1993 but I can wait until April
1993 to fill the position if necessary. I am also open to taking a Ph.D.
student if you have already taken a Master's degree.  


If you are interested please contact me via E-Mail. My address is
Jrichmn@ccu.umanitoba.ca 

I will answer all replies. 

Dr. Joy Richman
Dept. of Preventive Dental Science 
780 Bannatyne Avenue Winnipeg,
Manitoba, Canada
R3E 0W2