From: dfritz01@GUMEDLIB.DML.GEORGETOWN.EDU (DAVID FRITZINGER)
Newsgroups: bionet.jobs
Subject: CV
Date: 29 Sep 92 17:19:18 GMT
Distribution: bionet

To whom it may concern;

We do not get USENET news at Georgetown, so anyone interested in my CV
should contact me by phone, E-mail, or US mail.
 
Thank you
David C. Fritzinger
 
 
David C. Fritzinger, Ph.D.


                    CURRICULUM VITAE

Personal Information:

     Address:       6177  Old Brentford Ct.
                    Alexandria, VA 22310
                    (703)922-7610

     Business Address:   Dept. of Biochemistry and Molecular Biology
                    Georgetown University 
                    3900 Reservoir Road
                    Washington, D.C. 20007
                    (202)687-1444 (Lab)
                    (202)687-4398 (Office)
                    (202)687-7186 (Fax)
                    dfritz01@gumedlib.dml.georgetown.edu (E-mail)

     Citizenship:        United States

     Date of Birth:      January 14, 1951

     Marital Status:     Married

     Social Security Number:152-44-0330

Education:

Purdue University        B.S. Chemistry      1973
University of Massachusetts   Ph.D.     Biochemistry        1981

Objective:  Responsible, research-oriented position with biotechnology or phar
aceutical 
firm or government.  I prefer goal-oriented research with an emphasis on molec
lar biology 
(DNA cloning, sequencing, and gene expression).
Professional Experience:

August, 1988-Present:  Assistant Professor for Research, Dept. of Biochemistry
and 
Molecular Biology, Georgetown University Medical Center.  Head of project to s
udy the 
molecular biology of Cobra Venom Factor and cobra C3.  Supervised several 
undergraduates and graduate students and one post-doctoral research associate.
April, 1984-May, 1988: Scientist, Applied Microbiology, Inc.  Experience:  Par
icipated in 
research on gene expression in Bacillus subtilis, the design of shuttle vector
 for use in E. 
coli and Bacillus  species, production of recombinant proteins in Bacillus, an
 on the 
development of diagnostic assay systems.  
February, 1983- March, 1984:  Postdoctoral research associate in the laborator
es of Dr. 
M. J. Fournier and Dr. T. L. Mason, Department of Biochemistry, University of 
Massachusetts, Amherst, Massachusetts.  Project: Flavivirus Gene Structure and
Expression.

1981-1982:  Postdoctoral research associate in the laboratory of Dr. N. L. Bro
n at the 
University of Bristol (U. K.).  Project:  DNA sequence analysis of the Mercury
Resistance 
Transposon, Tn501.  Also taught in a DNA sequencing workshop (July, 1981, Univ
rsity 
of Bristol).
1974-1980  Graduate student in Laboratory of Dr. M. J. Fournier, Dept. of Bioc
emistry, 
University of Massachusetts (Amherst).  Thesis Title:  RA Chemical Modificatio
 Study of 
Changes in the Solution Structure of Yeast Phenylalanine-specific Transfer RNA
S  
Honors:  1977-1980; National Institutes of Health Cell and Molecular Biology  
raining 
grant awarded to the University of Massachusetts at Amherst.
Techniques:  Construction of expression systems, culturing of B. subtilis, clo
ing into B. 
subtilis, Western blotting for detection of antigens, purification, kinasing, 
nd ligation of 
oligonucleotides, in vivo and in vitro labeling of RNA and DNA, isolation of l
w 
molecular weight RNAs from yeast and bacteria, purification of single species 
f tRNA 
(both radioactively labeled and unlabeled), fingerprint analysis of RNA oligon
cleotides, 
base compositional analysis of tRNA, chemical modification of tRNA, enzyme iso
ation 
from yeast, bacteria, and mammalian tissue, fermentation, enzyme characterizat
on, one- 
and two-dimensional gel electrophoresis, column chromatography, HPLC (reverse-
hase 
chromatography), growth, isolation, and purification of bacteriophage, molecul
r cloning 
of DNA, DNA sequencing, plasmid preparation and purification, DNA restriction 
nalysis, 
isolation of total RNA and mRNA,  Northern analysis of RNA, cDNA cloning, Scre
ning 
of cDNA libraries by hybridization and immunological methods, Polymerase Chain
Reaction (PCR) amplification of DNA, use of PCR for library screening.


Publications:

Fritzinger, D. C, Bredehorst, R., and Vogel, C.-W. RComplete Sequence of a Sec
nd 
Cobra Venom Factor mRNA:  Comparison to Cobra Venom Factor and Cobra C3 
Sequences Reveals the Existence of 3 C3 Related GenesS  In Preparation.
Fritzinger, D. C, Bredehorst, R., and Vogel, C.-W. RPrimary Structure of Cobra
Venom 
Factor mRNA:  Comparison with Cobra C3 and Other C3 Sequences.S  In Preparatio
. 
Fritzinger, D. C., Connelly, M., Petrella, E. C., Bredehorst, R., and Vogel, C
-W.  
RPrimary Structure of Complement Component C3 from Cobra PlasmaS  J. Immunol.,
In 
Press.
Brown, N.L., Winnie, J.N., Fritzinger, D.C., and Pridmore, R.D. RThe Nucleotid
 
Sequence of the tnpA Gene Completes the Sequence of the Pseudomonal Transposon
Tn501.S  Nucleic Acids Res. 13, 5657-5669 (1985).

Misra, T K., Brown, N.L., Fritzinger, D.C., Pridmore, R.D., Barnes, W.M., 
Haberstroh, L., and Silver, S.  RMercuric Ion-Resistance Operons of Plasmid R1
0 and 
Tn501: the Beginning of the Operon Including the Regulatory Region and the Fir
t Two 
Structural Genes.S  Proc. Natl. Acad. Sci., USA 81, 5975-5979 (1984).
Brown, N.L., Pridmore, R.D., and Fritzinger, D.C.  RThe Mercury-Resistance Gen
s of 
Transposon Tn501: Nucleotide Sequence of the Mer Operon and a Possible Mechani
m for 
Mercury Detoxification.S  Biochem. Soc. Transactions, 604th Meeting, Cambridge
 276-
277, (1984).
Diver, W.P., Grinsted, J., Fritzinger, D.C., Brown, N.L., Altenbuchner, J., Ro
owsky, 
P., and Schmitt, R.  RDNA Sequences of and Complementation by the tnpR Genes o
 
Tn21, Tn501, and Tn1721.S  Molec. and Gen. Genetics 191, 189-193 (1983).
Brown, N.L., Ford, S.J., Pridmore, R.D., and Fritzinger, D.C.  RNucleotide Seq
ence of 
a Gene from the Pseudomonas Transposon Tn501 Encoding Mercuric Resistance.S 
Biochemistry 22, 4089-4095 (1983).
Fritzinger, D.C., and Fournier, M.J.  RCarbodiimide Modification Analysis of 
Aminoacylated Yeast Phenylalanine tRNA:  Evidence for Change in the Apex Regio
.S 
Nucleic Acids Res. 10, 2419-2437 (1982).
Fritzinger, D.C., and Fournier, M.J.,  RChemical Modification Analysis of IonP
ependent 
Changes in the Solution Structure of Yeast Phenylalanine Transfer Ribonucleic 
cid.S  
Biochemistry 21, 2118-2127 (1982).
Potts, R.O., Wang, C.C., Fritzinger, D.C., Ford, N.C., and Fournier, M.J.  REf
ects of 
Aminoacylation and Solution Conditions on the Structure of Transfer Ribonuclei
 AcidS. in 
Transfer RNA:  Structure, Properties and Recognition, Abelson, J., Schimmel, P
 and 
Sll, D. eds.  Cold Spring Harbor Laboratory, NY pp. 207-220 (1979).

Abstracts:

Fritzinger, D.C., Bredehorst, R., and Vogel, C.-W.  RComplete Sequence of Two 
Different Cobra Venom Factor cDNAs.S  FASEB J., 4, A1453 (1992).
Fritzinger, D., C.,  Petrella, E. C., Bredehorst, R. and Vogel, C.-W.  RComple
e Structure 
of Cobra Complement Component C3 mRNA.S  Complement and Inflammation, 8, 152 
(1991).
Fritzinger, D. C., Bredehorst, R., and Vogel, C.-W.  RSequence Analysis of Cob
a 
Venom Factor mRNA Reveals the Existence of Two Different mRNA Species.S  
Complement and Inflammation, 8, 152 (1991).
Fritzinger, D. C., Bredehorst, R., and Vogel, C.-W.  RComplete Structure of Co
ra 
Complement Component C3 mRNA.S  XIV International Congress of Biochemistry,  
Jerusalem, Israel,  August, 1991.
Fritzinger, D. C., Connelly, M.B., Petrella, E. C, Bredehorst, R., and Vogel, 
-W. 
RMolecular Cloning of Cobra Venom Factor and Cobra C3:  Structural Homology wi
h 
Human C3.S  FASEB J. 4, A1903 (1990).
Fritzinger, D. C., Bredehorst, R. and Vogel, C.-W.  RMolecular Cloning of Cobr
 Venom 
Factor and Cobra Complement Component C3:  Structural Homology with Human C3.S
 
Sixth Pan-American Biochemistry Congress, Sao Paulo, Brazil (1990).
Fritzinger, D.C., and Fournier, M.J.  RChemical Modification Analysis of Amino
cylated 
Transfer RNA.S Fed. Proc. 40, 1646 (1981).
Fritzinger, D.C., and Fournier, M.J.  RChemical Modification Analysis of Confo
mational 
Changes in Transfer RNA.S Fed. Proc. 39, 2023 (1980).
Potts, R., Fritzinger, D.C., Ford, N.C., and Fournier, M.J.  REffects of Amino
cylation 
and Magnesium on the Structure of Transfer Ribonucleic Acid.S Fed. Proc. 37, 1
32 
(1978).
Miscellaneous

Member American Association for the Advancement of Science.  Experienced using
Apple 
Macintosh computers and various Macintosh programs (including word processing,
graphics, and database programs).


Carl-Wilhelm Vogel, M.D., Ph.D.    Eugene A. Davidson, Ph.D.
Chairman, Dept. of Biochemistry    Chairman, Dept. of Biochemistry
University of Hamburg         and Molecular Biology
Martin Luther King Pl. 6      Georgetown University
2000 Hamburg 13               3900 Reservoir Rd.
Germany                  Washington, DC 20007
Phone:  49-40-41233214        Phone:  (202)687-1501
Fax:  49-40-41232848          Fax:  (202)687-7186

Reinhard Bredehorst, M.D.          M. J. Fournier, Ph.D.
Dept. of Biochemistry         Dept. of Biochemistry
University of Hamburg         GRC Twr. B
Martin Luther King Pl. 6      University of Massachusetts
2000 Hamburg 13               Amherst, MA  01003
Germany                  Phone:  (413)545-2732
Phone:  49-40-41232436
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