From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: C.botulinum gene for botulinum neurotoxin type E
Date: 25 Jul 92 10:03:04 GMT
Distribution: bionet
Checksum: 54863     8

LOCUS       CLONEUTOXE   4017 bp ds-DNA             BCT       25-JUL-1992
DEFINITION  C.botulinum gene for botulinum neurotoxin type E
ACCESSION   X62089
KEYWORDS    botulinum neurotoxin type E.
SOURCE      Clostridium botulinum DNA.
  ORGANISM  Clostridium botulinum
            Prokaryotae; Firmicutes; Endospore-forming rods and cocci;
            Bacillaceae.
REFERENCE   1  (bases 1 to 4017)
  AUTHORS   Poulet,S., Hauser,D., Quanz,M., Niemann,H. and Popoff,M.R.
  TITLE     Sequences of botulinal neurotoxin E gene derived from Clostridium
            botulinum type E (strain Beluga) and Clostridium butyricum (strains
            ATCC437555 and ATCC43181)
  JOURNAL   Biochem. Biophys. Res. Commun. 183, 107-113 (1992)
  STANDARD  full automatic
COMMENT     *source: strain=Beluga;
            Related sequence: M30196
            From EMBL    entry CBNEUTOXE;  dated 17-JUN-1992.
FEATURES             Location/Qualifiers
     RBS             215..220
     CDS             229..3984
                     /product="botulinum neurotoxin type E"
                     /codon_start=1
                     /translation="MPKINSFNYNDPVNDRTILYIKPGGCQEFYKSFNIMKNIWIIPE
                     RNVIGTTPQDFHPPTSLKNGDSSYYDPNYLQSDEEKDRFLKIVTKIFNRINNNLSGGI
                     LLEELSKANPYLGNDNTPDNQFHIGDASAVEIKFSNGSQDILLPNVIIMGAEPDLFET
                     NSSNISLRNNYMPSNHRFGSIAIVTFSPEYSFRFNDNCMNEFIQDPALTLMHELIHSL
                     HGLYGAKGITTKYTITQKQNPLITNIRGTNIEEFLTFGGTDLNIITSAQSNDIYTNLL
                     ADYKKIASKLSKVQVSNPLLNPYKDVFEAKYGLDKDASGIYSVNINKFNDIFKKLYSF
                     TEFDLRTKFQVKCRQTYIGQYKYFKLSNLLNDSIYNISEGYNINNLKVNFRGQNANLN
                     PRIITPITGRGLVKKIIRFCKNIVSVKGIRKSICIEINNGELFFVASENSYNDDNINT
                     PKEIDDTVTSNNNYENDLDQVILNFNSESAPGLSDEKLNLTIQNDAYIPKYDSNGTSD
                     IEQHDVNELNVFFYLDAQKVPEGENNVNLTSSIDTALLEQPKIYTFFSSEFINNVNKP
                     VQAALFVSWIQQVLVDFTTEANQKSTVDKIADISIVVPYIGLALNIGNEAQKGNFKDA
                     LELLGAGILLEFEPELLIPTILVFTIKSFLGSSDNKNKVIKAINNALKERDEKWKEVY
                     SFIVSNWMTKINTQFNKRKEQMYQALQNQVNAIKTIIESKYNSYTLEEKNELTNKYDI
                     KQIENELNQKVSIAMNNIDRFLTESSISYLMKIINEVKINKLREYDENVKTYLLNYII
                     QHGSILGESQQELNSMVTDTLNNSIPFKLSSYTDDKILISYFNKFFKRIKSSSVLNMR
                     YKNDKYVDTSGYDSNININGDVYKYPTNKNQFGIYNDKLSEVNISQNDYIIYDNKYKN
                     FSISFWVRIPNYDNKIVNVNNEYTIINCMRDNNSGWKVSLNHNEIIWTFEDNRGINQK
                     LAFNYGNANGISDYINKWIFVTITNDRLGDSKLYINGNLIDQKSILNLGNIHVSDNIL
                     FKIVNCSYTRYIGIRYFNIFDKELDETEIQTLYSNEPNTNILKDFWGNYLLYDKEYYL
                     LNVLKPNNFIDRRKDSTLSINNIRSTILLANRLYSGIKVKIQRVNNSSTNDNLVRKND
                     QVYINFVASKTHLFPLYADTATTNKEKTIKISSSGNRFNQVVVMNSVGNCTMNFKNNN
                     GNNIGLLGFKADTVVASTWYYTHMRDHTNSNGCFWNFISEEHGWQEK"
BASE COUNT     1673 a    430 c    565 g   1349 t
ORIGIN
        1 gaattcaagt agtagataat aaaaataatg ccacagattt ttattattaa taatgatata
       61 tttatctcta actgtttaac tttaacttat aacaatgtaa atatatattt gtctataaaa
      121 aatcaagatt acaattgggt tatatgtgat cttaatcatg atataccaaa aaagtcatat
      181 ctatggatat taaaaaatat ataaatttaa aattaggaga tgctgtatat gccaaaaatt
      241 aatagtttta attataatga tcctgttaat gatagaacaa ttttatatat taaaccaggc
      301 ggttgtcaag aattttataa atcatttaat attatgaaaa atatttggat aattccagag
      361 agaaatgtaa ttggtacaac cccccaagat tttcatccgc ctacttcatt aaaaaatgga
      421 gatagtagtt attatgaccc taattattta caaagtgatg aagaaaagga tagattttta
      481 aaaatagtca caaaaatatt taatagaata aataataatc tttcaggagg gattttatta
      541 gaagaactgt caaaagctaa tccatattta gggaatgata atactccaga taatcaattc
      601 catattggtg atgcatcagc agttgagatt aaattctcaa atggtagcca agacatacta
      661 ttacctaatg ttattataat gggagcagag cctgatttat ttgaaactaa cagttccaat
      721 atttctctaa gaaataatta tatgccaagc aatcaccgtt ttggatcaat agctatagta
      781 acattctcac ctgaatattc ttttagattt aatgataatt gtatgaatga atttattcaa
      841 gatcctgctc ttacattaat gcatgaatta atacattcat tacatggact atatggggct
      901 aaagggatta ctacaaagta tactataaca caaaaacaaa atcccctaat aacaaatata
      961 agaggtacaa atattgaaga attcttaact tttggaggta ctgatttaaa cattattact
     1021 agtgctcagt ccaatgatat ctatactaat cttctagctg attataaaaa aatagcgtct
     1081 aaacttagca aagtacaagt atctaatcca ctacttaatc cttataaaga tgtttttgaa
     1141 gcaaagtatg gattagataa agatgctagc ggaatttatt cggtaaatat aaacaaattt
     1201 aatgatattt ttaaaaaatt atacagcttt acggaatttg atttacgaac taaatttcaa
     1261 gttaaatgta ggcaaactta tattggacag tataaatact tcaaactttc aaacttgtta
     1321 aatgattcta tttataatat atcagaaggc tataatataa ataatttaaa ggtaaatttt
     1381 agaggacaga atgcaaattt aaatcctaga attattacac caattacagg tagaggacta
     1441 gtaaaaaaaa tcattagatt ttgtaaaaat attgtttctg taaaaggcat aaggaaatca
     1501 atatgtatcg aaataaataa tggtgagtta ttttttgtgg cttccgagaa tagttataat
     1561 gatgataata taaatactcc taaagaaatt gacgatacag taacttcaaa taataattat
     1621 gaaaatgatt tagatcaggt tattttaaat tttaatagtg aatcagcacc tggactttca
     1681 gatgaaaaat taaatttaac tatccaaaat gatgcttata taccaaaata tgattctaat
     1741 ggaacaagtg atatagaaca acatgatgtt aatgaactta atgtattttt ctatttagat
     1801 gcacagaaag tgcccgaagg tgaaaataat gtcaatctca cctcttcaat tgatacagca
     1861 ttattagaac aacctaaaat atatacattt ttttcatcag aatttattaa taatgtcaat
     1921 aaacctgtgc aagcagcatt atttgtaagc tggatacaac aagtgttagt agattttact
     1981 actgaagcta accaaaaaag tactgttgat aaaattgcag atatttctat agttgttcca
     2041 tatataggtc ttgctttaaa tataggaaat gaagcacaaa aaggaaattt taaagatgca
     2101 cttgaattat taggagcagg tattttatta gaatttgaac ccgagctttt aattcctaca
     2161 attttagtat tcacgataaa atctttttta ggttcatctg ataataaaaa taaagttatt
     2221 aaagcaataa ataatgcatt gaaagaaaga gatgaaaaat ggaaagaagt atatagtttt
     2281 atagtatcga attggatgac taaaattaat acacaattta ataaaagaaa agaacaaatg
     2341 tatcaagctt tacaaaatca agtaaatgca attaaaacaa taatagaatc taagtataat
     2401 agttatactt tagaggaaaa aaatgagctt acaaataaat atgatattaa gcaaatagaa
     2461 aatgaactta atcaaaaggt ttctatagca atgaataata tagacaggtt cttaactgaa
     2521 agttctatat cctatttaat gaaaataata aatgaagtaa aaattaataa attaagagaa
     2581 tatgatgaga atgtcaaaac gtatttattg aattatatta tacaacatgg atcaatcttg
     2641 ggagagagtc agcaagaact aaattctatg gtaactgata ccctaaataa tagtattcct
     2701 tttaagcttt cttcttatac agatgataaa attttaattt catattttaa taaattcttt
     2761 aagagaatta aaagtagttc agttttaaat atgagatata aaaatgataa atacgtagat
     2821 acttcaggat atgattcaaa tataaatatt aatggagatg tatataaata tccaactaat
     2881 aaaaatcaat ttggaatata taatgataaa cttagtgaag ttaatatatc tcaaaatgat
     2941 tacattatat atgataataa atataaaaat tttagtatta gtttttgggt aagaattcct
     3001 aactatgata ataagatagt aaatgttaat aatgaataca ctataataaa ttgtatgaga
     3061 gataataatt caggatggaa agtatctctt aatcataatg aaataatttg gacattcgaa
     3121 gataatcgag gaattaatca aaaattagca tttaactatg gtaacgcaaa tggtatttct
     3181 gattatataa ataagtggat ttttgtaact ataactaatg atagattagg agattctaaa
     3241 ctttatatta atggaaattt aatagatcaa aaatcaattt taaatttagg taatattcat
     3301 gttagtgaca atatattatt taaaatagtt aattgtagtt atacaagata tattggtatt
     3361 agatatttta atatttttga taaagaatta gatgaaacag aaattcaaac tttatatagc
     3421 aatgaaccta atacaaatat tttgaaggat ttttggggaa attatttgct ttatgacaaa
     3481 gaatactatt tattaaatgt gttaaaacca aataacttta ttgataggag aaaagattct
     3541 actttaagca ttaataatat aagaagcact attcttttag ctaatagatt atatagtgga
     3601 ataaaagtta aaatacaaag agttaataat agtagtacta acgataatct tgttagaaag
     3661 aatgatcagg tatatattaa ttttgtagcc agcaaaactc acttatttcc attatatgct
     3721 gatacagcta ccacaaataa agagaaaaca ataaaaatat catcatctgg caatagattt
     3781 aatcaagtag tagttatgaa ttcagtagga aattgtacaa tgaattttaa aaataataat
     3841 ggaaataata ttgggttgtt aggtttcaag gcagatactg tcgttgctag tacttggtat
     3901 tatacacata tgagagatca tacaaacagc aatggatgtt tttggaactt tatttctgaa
     3961 gaacatggat ggcaagaaaa ataaaaatta gattaaacgg ctaaagtcat aaattcc
//