From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: T.aquaticus tufA and fus genes for elongation factor Tu and
Date: 4 Sep 92 10:07:20 GMT
Distribution: bionet
Checksum: 56227     4

LOCUS       TTHEFTUG     1347 bp ds-DNA             BCT       04-SEP-1992
DEFINITION  T.aquaticus tufA and fus genes for elongation factor Tu and
            elongation factor G (partial)
ACCESSION   X66322
KEYWORDS    elongation factor G; elongation factor TU; fus gene; tufa gene.
SOURCE      Thermus aquaticus DNA.
  ORGANISM  Thermus aquaticus
            Prokaryotae; Gracilicutes; Scotobacteria; Neisseriaceae.
REFERENCE   1  (bases 1 to 1347)
  AUTHORS   Erdmann,V.A.
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
REFERENCE   2  (bases 31 to 1347)
  AUTHORS   Voss,R.H., Hartmann,R.K., Lippmann,C., Alexander,C.M., Jahn,O. and
            Erdmann,V.A.
  TITLE     Sequence of the tufA gene encoding elogation factor EF-Tu from
            Thermus aquaticus and overproduction of the protein in Escherichia
            coli
  JOURNAL   Eur. J. Biochem. 207, 839-846 (1992)
  STANDARD  full automatic
COMMENT     *source: strain=EP 00276;
            From EMBL    entry TAEFTUG;  dated 06-AUG-1992.
FEATURES             Location/Qualifiers
     CDS             <1..54
                     /product="elongationfactor G"
                     /gene="fus"
                     /codon_start=1
                     /translation="HYQEVPRQIQEKLIKGQ"
     RBS             100..107
                     /gene="tufA"
     CDS             115..1335
                     /product="elongation factor Tu"
                     /gene="tufA"
                     /codon_start=1
                     /translation="MAKGEFIRTKPHVNVGTIGHVDHGKTTLTAALTYVAAAENPNVE
                     VKDYGDIDKAPEERARGITINTAHVEYETAKRHYSHVDCPGHADYIKNMITGAAQMDG
                     AILVVSAADGPMPQTREHILLARQVGVPYIVVFMNKVDMVDDPELLDLVEMEVRDLLN
                     QYEFPGDEVPVIRGSALLALEEMHKNPKTKRGENEWVDKIWELLDAIDEYIPTPVRDV
                     DKPFLMPVEDVFTITGRGTVATGRIERGKVKVGDEVEIVGLAPETRKTVVTGVEMHRK
                     TLQEGIAGDNVGLLLRGVSREEVERGQVLAKPGSITPHTKFEASVYILKKEEGGRHTG
                     FFTGYRPQFYFRTTDVTGVVRLPQGVEMVMPGDNVTFTVELIKPVALEEGLRFAIREG
                     GRTVGAGVVTKILE"
BASE COUNT      265 a    318 c    542 g    222 t
ORIGIN
        1 cactaccagg aggtccccag gcagatccag gagaagctca tcaaggggca gtaggaaggc
       61 tttgggggtg ggtctcctgg gcccacccca cgctttgcgg aaaggagagc gaagatggcg
      121 aagggcgagt ttatccggac gaagccccac gtgaacgtgg ggacgattgg gcacgtggac
      181 cacgggaaga cgacgttgac ggcggccttg acctatgtgg cggcggcgga gaacccgaat
      241 gtggaggtga aggactacgg ggacattgac aaggcgccgg aggagcgggc gcgggggatc
      301 acgattaaca cggcgcacgt ggagtacgag acggcgaagc ggcactattc ccacgtggac
      361 tgcccggggc acgccgacta catcaagaac atgatcaccg gggcggcgca gatggacggg
      421 gcgatcctgg tggtttctgc ggcggacggt cctatgccgc agacccggga gcacattctt
      481 ttggcccgcc aggtgggggt gccgtacatc gtggtgttca tgaacaaggt ggacatggtg
      541 gacgacccgg agctcttgga cctggtggag atggaggtga gggacctcct gaaccagtac
      601 gagtttcctg gggacgaggt gccggtgatc cggggtagcg ccctgcttgc gttggaagag
      661 atgcacaaga acccgaagac caagcggggc gagaacgagt gggtggacaa gatctgggag
      721 cttttggacg ccattgacga gtacattccc acgccggtgc gggacgtgga caagcctttc
      781 ctgatgccgg tggaggacgt gtttacgatt accgggcgtg ggacggtggc caccgggcgg
      841 attgagcggg gcaaggtgaa ggttggggac gaggtggaga tcgtgggcct ggctccggag
      901 acgcggaaga cggtggtcac gggtgtggag atgcaccgga agacgttgca ggaggggatc
      961 gctggggaca acgtgggtct tcttctgcgg ggcgtgagcc gggaggaggt ggagcggggg
     1021 caggtattgg ccaagcctgg gagcatcacg ccgcacacca agtttgaggc ctcggtgtac
     1081 atcctgaaga aggaggaggg cgggcggcac acggggtttt tcacggggta ccggccgcag
     1141 ttttacttcc ggacgacgga cgtgacgggg gtggtgcggt tgccccaggg ggtggagatg
     1201 gtgatgcctg gggacaacgt gaccttcacg gtggagctca tcaagccggt ggcgctggag
     1261 gagggtttgc ggtttgccat ccgggagggt gggcggaccg tgggcgccgg cgtggtcacc
     1321 aagatcctgg agtgaggtga agcatgc
//