From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: beta-methylaspartase [Clostridium tetanomorphum, H1, ATCC 15920,
Date: 27 Jan 93 05:56:17 GMT
Distribution: bionet
Checksum: 16226 6

LOCUS       S118074      2244 bp    DNA             BCT       13-JAN-1993
DEFINITION  beta-methylaspartase [Clostridium tetanomorphum, H1, ATCC 15920,
            Genomic, 2244 nt]
ACCESSION   S48141
KEYWORDS    .
SOURCE      Clostridium tetanomorphum H1 ATCC 15920
  ORGANISM  Clostridium tetanomorphum
            Unclassified.
REFERENCE   1  (bases 1 to 2244)
  AUTHORS   Goda,S.K., Minton,N.P., Botting,N.P. and Gani,D.
  TITLE     Cloning, sequencing, and expression in Escherichia coli of the
            Clostridium tetanomorphum gene encoding beta-methylaspartase and
            characterization of the recombinant protein.
  JOURNAL   Biochemistry 31, 10747-10756 (1992)
  STANDARD  full automatic
COMMENT     This entry [NCBI gibbsq 118074] was created by the journal scanning
            component of NCBI/GenBank at the National Library of Medicine.
            This sequence comes from Fig. 2.
FEATURES             Location/Qualifiers
     CDS             3..629
                     /partial
                     /note="Description: orf A 5' of methylaspartase;  For the
                     protein sequence (NCBI gibbsq 118075):  Method: conceptual
                     translation supplied by author.  This sequence comes from
                     Fig. 2."
                     /codon_start=1
                     /translation="AYGYNDVFVTTVFHQWMGGFPQDESKAFGVIVTATTIASLAGAT
                     KVIVKTPHEAIGIPTKEANASGIKATKMALNMLEGQRMPMSKELETEMAIIKAETKCI
                     LDKMFELGKGDLAVGTVKAFETGVMDIPFGPSKYNAGKMMPVRDNLGCVRYLEFGNVP
                     FTEELKNYNRERLAERAKFEGREVSFQMVIDDIFAVGKGRLIGRPENK"
     CDS             756..1997
                     /note="3-methylaspartate ammonia-lyase;  For the protein
                     sequence (NCBI gibbsq 118076):  Method: conceptual
                     translation with partial peptide sequencing.  This
                     sequence comes from Fig. 2."
                     /product="beta-methylaspartase"
                     /codon_start=1
                     /translation="MKIVDVLCTPGLTGFYFDDQRAIKKGAGHDGFTYTGSTVTEGFT
                     QVRQKGESISVLLVLEDGQVAHGDCAAVQYSGAGGRDPLFLAKDFIPVIEKEIAPKLI
                     GREITNFKPMAEEFDKMTVNGNRLHTAIRYGITQAILDAVAKTRKVTMAEVIRDEYNP
                     GAEINAVPVFAQSGDDRYDNVDKMIIKEADVLPHALINNVEEKLGLKGEKLLEYVKWL
                     RDRIIKLRVREDYAPIFHIDVYGTIGAAFDVDIKAMADYIQTLAEAAKPFHLRIEGPM
                     DVEDRQKQMEAMRDLRAELDGRGVDAELVADEWCNTVEDVKFFTDNKAGHMVQIKTPD
                     LGGVNNIADAIMYCKANGMGAYCGGTCNETNRSAEVTTNIGMACGARQVLAKPGMGVD
                     EGMMIVKNEMNRVLALVGRRK"
BASE COUNT      846 a    305 c    479 g    614 t
ORIGIN      
        1 aagcttatgg atacaatgat gtatttgtaa caacagtatt ccatcagtgg atgggtggat
       61 tccctcaaga tgaatccaaa gcatttggcg ttatagtaac agctacaact atagcatcat
      121 tagcaggagc aactaaagtt atagttaaga ctccacatga agctattggt ataccaacaa
      181 aagaagctaa tgcttcaggt atcaaagcta caaagatggc attaaatatg ttagaaggac
      241 agagaatgcc aatgtcaaaa gaattagaaa ctgaaatggc aattataaaa gctgaaacta
      301 aatgcatact tgataagatg tttgaattag gaaaaggtga tttagcagta ggtactgtta
      361 aagcattcga aactggtgtt atggatatac catttggacc aagcaaatac aatgcaggaa
      421 aaatgatgcc agttagagac aacttaggat gcgtaagata cctagaattc ggtaacgttc
      481 catttactga agaattaaag aattataaca gagaaagatt agctgaaaga gctaaattcg
      541 aaggaagaga agttagcttc cagatggtta ttgatgatat atttgcagta ggtaaaggaa
      601 gacttatcgg aagaccagaa aataaataat ttataagacc ttgtttatta ccacatttaa
      661 tgctggcagg aaactgtcag catggatgtg gataaaaata tataataaca attagttgtt
      721 aaattttatt aaaaaaaaag gacaggtgaa taattatgaa aattgttgac gtactttgta
      781 caccaggatt aactggattc tattttgatg accaaagagc aatcaaaaag ggagcaggac
      841 atgatggatt tacatatact ggctctactg taacagaagg atttactcaa gtaagacaaa
      901 aaggtgaatc aatatctgta ttattagttc ttgaagatgg tcaggttgct cacggagatt
      961 gtgctgcagt tcagtactca ggagcaggcg gaagagatcc attattctta gctaaagatt
     1021 ttatacctgt tatagaaaaa gaaatagctc caaaattgat tggaagagaa ataactaact
     1081 ttaaaccaat ggcagaagaa ttcgacaaaa tgactgtaaa tggtaataga ttacatactg
     1141 ctatcagata tggtataact caggctatac ttgatgcagt agctaaaact agaaaagtaa
     1201 ctatggctga agtaatcaga gatgaataca atccaggagc agaaatcaat gcagttcctg
     1261 tatttgctca gtcaggtgat gatagatacg ataacgttga taagatgata attaaagaag
     1321 ctgatgtttt accacatgct ttaatcaaca atgttgaaga aaaattagga cttaaaggag
     1381 aaaaacttct tgagtacgtt aaatggttaa gagatagaat aatcaaatta agagtaagag
     1441 aagactatgc tccaatattc cacatcgacg tatatggaac aatcggtgct gcatttgatg
     1501 ttgacatcaa ggctatggct gattacattc agactttagc tgaagctgct aagccattcc
     1561 acttaagaat tgaaggacca atggacgttg aagatagaca aaaacaaatg gaagctatga
     1621 gagacttaag agctgaatta gacggaagag gagtagacgc agaattagtt gctgatgaat
     1681 ggtgtaatac agttgaagat gttaaattct tcactgataa caaagctgga cacatggttc
     1741 agatcaaaac tccagacctt ggtggagtta acaatatagc agatgctatt atgtactgta
     1801 aagctaacgg aatgggagct tattgtggag gaacttgtaa cgaaactaac agatccgctg
     1861 aagttacaac taacataggt atggcttgtg gagctagaca ggttcttgct aaaccaggta
     1921 tgggtgttga cgaaggaatg atgatcgtta agaacgaaat gaacagagtt ttagctcttg
     1981 taggaagaag aaaataatta tatagggtta tacaacttag gtttttgaag aggcggttta
     2041 cttttaaact gccttttcaa ctataaatgg ctaattaatc atattttgaa aggaggcatt
     2101 tatctagcaa gtgaattaaa cctacacttg cttggtaaat atgcatatat gaaagaagta
     2161 aatgttgcag agataacaaa ggctataaaa gaattatgta tagaagctaa ctatagactt
     2221 cctgctgatg ttaaagcaaa gctt
//