From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: S. marcescens thrA1+/A2-2 gene encoding aspartokinase-homoserine
Date: 21 Jul 92 10:08:25 GMT
Distribution: bionet
Checksum: 03273     8

LOCUS       SMATHR2      2787 bp ds-DNA             BCT       21-JUL-1992
DEFINITION  S. marcescens thrA1+/A2-2 gene encoding aspartokinase-homoserine
            dehydrogenase.
ACCESSION   D10386
KEYWORDS    aspartokinase I; homoserine dehydrogenase I.
SOURCE      Serratia marcescens (clone pMT401, individual_isolate HNr59, strain
            Sr41) DNA.
  ORGANISM  Serratia marcescens
            Prokaryotae; Gram-negative facultatively anaerobic rods;
            Enterobacteriaceae.
REFERENCE   1  (bases 1 to 2787)
  AUTHORS   Omori,K., Suzuki,S. and Komatsubara,S.
  TITLE     Analysis of the thrA1A2BC genes of Serratia marcescens Sr41
  JOURNAL   Unpublished (1991)
  STANDARD  full staff_review
COMMENT     These data kindly submitted in computer readable form by:
            Kenji Omori
            Research Laboratory of Applied Biochemistry
            Tanabe Seiyaku Co., Ltd.
            3-16-89 Kashima
            Yodagawa-ku, Osaka 532
            Japan
            Phone:  06-300-2591
            Fax:    06-300-2593
FEATURES             Location/Qualifiers
     RBS             53..56
     CDS             69..137
                     /function="attenuation control"
                     /product="leader peptide"
                     /codon_start=1
                     /translation="MRNISLTTTIITTTDTTGNGAG"
     repeat_region   126..170
                     /rpt_type=inverted
                     /rpt_unit=126..135
                     /rpt_unit=161..170
     repeat_region   156..190
                     /rpt_type=inverted
                     /rpt_unit=156..169
                     /rpt_unit=177..190
     RBS             205..208
     CDS             218..2677
                     /EC_number="2.7.2.4"
                     /note="fused-type enzyme"
                     /product="aspartokinase I"
                     /gene="thrA1+/A2-2"
                     /codon_start=1
                     /translation="MRVLKFGGTSVANAERFLRVADIMESNARQGQVATVLSAPAKIT
                     NHLVAMIDKTVAGQDILPNMSDAERIFADLLSGLAQALPGFEYDRLKGVVDQEFAQLK
                     QVLHGVSLLGQCPDSVNAAIICRGEKLSIAIMEGVFRAKGYPVTVINPVEKLLAQGHY
                     LESTVDIAESTLRIAAAAIPADHIVLMAGFTAGNDKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRTVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTSNPQAPGTLIGKDSTDADMPVKGITNLNNMAMINVSGPGMKGMVGMAAR
                     VFAVMSRAGISVVLITQSSSEYSISFCVPQGELQRARRALEEEFYLELKDGVLDPLDV
                     MERLAIISVVGDGMRTLRGISARFFSALARANINIVAIAQGSSERSISVVVSNDSATT
                     GVRVSHQMLFNTDQVIEVFVIGVGGVGGTLIEQIYRQQPWLKQKHIDLRVCGIANSRV
                     MLTNVHGIALDSWRDALAGAQEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYVD
                     FLADGFHVVTPNKKANTSSMNYYQQLRAAAAGSHRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELVRFSGILSGSLSFIFGKLDEGLSLSAATLQARANGYTEPDPRDDLSGMDVARK
                     LLILAREAGYKLELSDIEVEPVLPPSFDASGDVDTFLARLPELDKEFARNVANAAEQG
                     KVLRYVGLIDEGRCKVRIEAVDGNDPLYKVKNGENALAFYSRYYQPLPLVLRGYGAGN
                     DVTAAGVFADLLRTLSWKLGV"
     CDS             218..2677
                     /EC_number="1.1.1.3"
                     /note="fused-type enzyme"
                     /product="homoserine dehydrogenase I"
                     /gene="thrA1+/A2-2"
                     /codon_start=1
                     /translation="MRVLKFGGTSVANAERFLRVADIMESNARQGQVATVLSAPAKIT
                     NHLVAMIDKTVAGQDILPNMSDAERIFADLLSGLAQALPGFEYDRLKGVVDQEFAQLK
                     QVLHGVSLLGQCPDSVNAAIICRGEKLSIAIMEGVFRAKGYPVTVINPVEKLLAQGHY
                     LESTVDIAESTLRIAAAAIPADHIVLMAGFTAGNDKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRTVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTSNPQAPGTLIGKDSTDADMPVKGITNLNNMAMINVSGPGMKGMVGMAAR
                     VFAVMSRAGISVVLITQSSSEYSISFCVPQGELQRARRALEEEFYLELKDGVLDPLDV
                     MERLAIISVVGDGMRTLRGISARFFSALARANINIVAIAQGSSERSISVVVSNDSATT
                     GVRVSHQMLFNTDQVIEVFVIGVGGVGGTLIEQIYRQQPWLKQKHIDLRVCGIANSRV
                     MLTNVHGIALDSWRDALAGAQEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYVD
                     FLADGFHVVTPNKKANTSSMNYYQQLRAAAAGSHRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELVRFSGILSGSLSFIFGKLDEGLSLSAATLQARANGYTEPDPRDDLSGMDVARK
                     LLILAREAGYKLELSDIEVEPVLPPSFDASGDVDTFLARLPELDKEFARNVANAAEQG
                     KVLRYVGLIDEGRCKVRIEAVDGNDPLYKVKNGENALAFYSRYYQPLPLVLRGYGAGN
                     DVTAAGVFADLLRTLSWKLGV"
     mutation        1652
                     /note="wild type"
BASE COUNT      550 a    824 c    873 g    540 t
ORIGIN
        1 ttgacttggg atagcgattg ctttaaccaa tagaggggac agagtatttc atgagacaga
       61 cagacaacat gcgcaatatc agcctgacta ccacaattat taccaccacc gataccacag
      121 gtaacggggc gggctgacgc gcacaggaaa cacagaaaaa agcccgcacc taatcagtgc
      181 gggctttttt ttcggcttaa attcggagag atcacaaatg cgagtgctga aatttggcgg
      241 aacctcggta gcgaatgcgg aacgttttct gcgcgtcgcc gacatcatgg agagtaacgc
      301 gcgtcaggga caggtagcca cggtcttgtc cgcccccgca aagatcacca accatctggt
      361 ggcgatgatc gacaaaacgg tggcgggcca ggacattctg ccgaatatga gcgacgccga
      421 gcggatcttt gccgacctgc tgagcggact ggcgcaggcg ctgccgggct ttgaatacga
      481 tcgtttgaaa ggcgtggtcg atcaggaatt cgcgcagctc aaacaggtgc tgcatggcgt
      541 ctcgctgctg gggcagtgcc cggacagcgt gaacgcggcg atcatctgcc gtggcgaaaa
      601 gctctccatc gccatcatgg aaggggtgtt ccgcgccaag ggttatccgg tcacggtgat
      661 caacccggtg gagaaactgc tggcgcaggg ccactacctg gagtccaccg tggacatcgc
      721 cgagtctacg ctgcgcatcg ccgccgcggc gatcccggcc gatcacatcg tgctgatggc
      781 cggtttcacc gccggtaatg ataagggcga gctggtggtg ctgggccgca acggctccga
      841 ctattccgcc gcggtgctgg ccgcctgcct gcgcgccgac tgttgcgaga tctggaccga
      901 cgtcgacggc gtttatacct gcgatccgcg caccgtgccg gacgccaggt tactgaaatc
      961 gatgtcgtac caggaagcga tggagctttc ctatttcggc gccaaagtgt tacacccccg
     1021 caccatcact ccgattgccc agtttcaaat cccttgcctg attaaaaaca cctccaaccc
     1081 gcaggccccc ggcacgctga tcggcaaaga cagcaccgat gccgatatgc cggtgaaggg
     1141 catcaccaac ctgaacaaca tggcgatgat caacgtctcc ggcccgggca tgaaaggcat
     1201 ggtcggcatg gccgcacggg tcttcgccgt gatgtcgcgc gccggcattt cggtggtgct
     1261 gatcacccaa tcctcttccg aatacagcat cagcttctgc gtgccgcagg gtgaactgca
     1321 gcgcgcccgt cgcgcgctgg aagaggagtt ctatctggag ctgaaagacg gcgtgctgga
     1381 tccgctggac gtgatggaac gcctggcgat catctcggtg gttggcgacg gcatgcgcac
     1441 cctgcgcggc atttccgcac gcttcttctc cgcgctggcg cgcgccaaca tcaatatcgt
     1501 tgccatcgcc caagggtctt ccgaacgttc catttcggtg gtggtcagca atgattccgc
     1561 caccaccggc gtgcgcgtca gccaccagat gctgttcaac accgatcagg tgatcgaagt
     1621 gttcgtcatc ggcgtcggcg gcgtcggcgg gacgctgatc gagcagatct atcgccagca
     1681 gccgtggctg aagcaaaaac atatcgatct gcgggtgtgc ggcatcgcca actcgcgcgt
     1741 gatgctgacc aatgtgcacg gcatcgcgct ggacagctgg cgcgacgcgc tggccggcgc
     1801 gcaggagccg ttcaatctcg gccgtctgat ccggctggtg aaggaatatc acctgctgaa
     1861 cccggtgatc gtcgactgta cctccagtca ggcggtggcc gatcagtacg tggacttcct
     1921 ggcggacggc ttccatgtgg tgacgccgaa caaaaaggcc aacacctcgt cgatgaacta
     1981 ttatcagcaa ctgcgcgcgg ccgccgccgg ttcgcatcgc aagttcctgt acgacaccaa
     2041 cgtcggtgcc ggtctgccgg tgattgagaa cctgcaaaac ctgctgaacg ccggtgatga
     2101 actggtacgt ttctccggta tcctgtccgg ctcgctgtcc tttatcttcg gcaagctgga
     2161 cgaagggctg tcgctgtcgg cggcgaccct gcaggcccgg gccaacggct acaccgagcc
     2221 ggatccgcgc gacgatctgt ccgggatgga tgtggcgcgt aagctgctga tcctggcgcg
     2281 cgaggccggt tacaaactgg aactgagcga tatcgaggtc gagccggtgc tgccgccgtc
     2341 cttcgacgcg tcgggcgacg tggacacctt cctggcgcgg ttgccggagc tggataaaga
     2401 gttcgcgcgc aacgtggcta acgccgccga gcaaggcaag gtgctgcgct atgtcggcct
     2461 gatcgacgaa gggcgctgca aggtgcgcat tgaggcggtg gacggcaacg atccgttgta
     2521 taaagtgaag aacggcgaga acgcgctggc cttctacagc cgctactatc agccgttgcc
     2581 gctggtgctg cgcggctacg gcgccggtaa cgatgtgact gcagcgggcg tattcgccga
     2641 tctgctgcgc acactgtcat ggaagttggg agtttaatat ggttaaggtg tatgcaccgg
     2701 cctcgatcgg caacgtcagc gtcggtttcg acgtgctggg cgcggcggtt tcgccgatcg
     2761 acggcacgct gctgggcgac tgtgtca
//