From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Sarcocystis muris 31-kDa major surface antigen mRNA, complete cds.
Date: 21 Aug 92 10:12:48 GMT
Distribution: bionet
Checksum: 34413     5

LOCUS       SAR31MSA     1535 bp ss-mRNA            INV       21-AUG-1992
DEFINITION  Sarcocystis muris 31-kDa major surface antigen mRNA, complete cds.
ACCESSION   M76496
KEYWORDS    major surface antigen.
SOURCE      Sarcocystis muris cyst merozoites cDNA to mRNA.
  ORGANISM  Sarcocystis muris
            Eukaryota; Animalia; Protozoa; Apicomplexa; Perkinsea; Coccidia;
            Eucoccidiidae; Eimeriina; Sarcocystidae.
REFERENCE   1  (bases 1 to 1535)
  AUTHORS   Eschenbacher,K.-H., Sommer,I., Meyer,H.E., Mehlhorn,H. and
            Rueger,W.
  TITLE     Cloning and expression in Escherichia coli of cDNAs encoding a
            31-kilodalton surface antigen of Sarcocystis muris
  JOURNAL   Mol. Biochem. Parasitol. 53, 159-168 (1992)
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     mRNA            1..1535
                     /note="putative"
                     /citation=[1]
     5'UTR           1..329
                     /note="putative"
                     /citation=[1]
     RBS             312..317
                     /note="presence of a prokaryotic RBS within a eukaryotic
                     (protozoic) mRNA"
                     /note="putative"
                     /citation=[1]
     sig_peptide     330..386
                     /note="putative"
                     /codon_start=1
                     /citation=[1]
     CDS             330..1172
                     /product="31-kDa major surface antigen"
                     /note="putative"
                     /codon_start=1
                     /translation="MAAVVEVILAVMVVFLWPLTSVASSNVSSTLQCDKTNKRLATET
                     ISTPQATLKLACPSSTTFLPTYTGDAGTQTVYLTQDGSSTEKLQTALPGATAKQEDSQ
                     TNEMTLTFPQLPDTSQTVYFHCLGTENIAGQGSRKEVCGFAVTLTAPPPQGPQACVVP
                     GTTIRLGIANEGDTTRFTCGGDLKLSPTAADKVFKEDCSTEESLKDLKRSEDKNSYFV
                     LTATKTPSKTTHCYLCEPDPTKKGHNDKNCAVLIAVGAGSRPTARSVFGVAAPCILAL
                     LHFT"
     mat_peptide     387..1169
                     /product="31-kDa major surface antigen"
                     /note="putative"
                     /codon_start=1
                     /citation=[1]
     misc_feature    1122..1169
                     /note="possible GPI anchor attachment site"
     3'UTR           1173..1534
                     /note="putative"
                     /citation=[1]
     polyA_site      1535
                     /note="putative"
                     /citation=[1]
BASE COUNT      400 a    363 c    388 g    384 t
ORIGIN
        1 tttgctcctg ccggacagcc ggcgttgttc gcgttatttc ttttaaggac attcgcactg
       61 gacgatccgc cgaacaatca agtctttgga ggtctgaaac cggctgtgct cttcctcgca
      121 gtgtgaggtt ttgtgcgcga ttggttgaag aggcttgctg tatggctgcg aagctgcgct
      181 cacacagttc agttggggac agccataatt ctccagtcac ctgttgcgaa gggattacca
      241 ggagaagtgt ttgcggaaag aggagtgggt tacacattct tgccgtaaaa gtaaggattg
      301 cgtgagttca tgagaggctt agttttggga tggcggcggt tgtagaagtt atcttggctg
      361 tcatggtggt gttcctttgg ccattgactt ctgttgcatc ctctaatgtt tccagtactt
      421 tacaatgcga taagactaat aaaaggctcg cgacagaaac tattagtaca cctcaggcaa
      481 cgctgaagct ggcgtgccca tcaagcacca cattccttcc aacttataca ggagacgcgg
      541 gcactcaaac tgtataccta acgcaagatg gctcgagcac agagaagctg caaactgcgt
      601 taccaggggc aacagcaaaa caggaagaca gccaaacaaa tgagatgacc cttacctttc
      661 cgcagctgcc agatacttcg cagaccgtgt acttccactg cctaggaact gagaacatcg
      721 ctgggcaggg gtcaagaaag gaagtctgcg gcttcgcagt gacattgacg gcaccacccc
      781 cgcaaggtcc acaggcatgc gttgttcctg gcaccactat caggttggga attgccaacg
      841 aaggagacac gacgcggttt acttgtggag gtgacctaaa gttgagcccg acggctgcag
      901 ataaggtttt caaggaagac tgctccacgg aagagtcgct gaaagatttg aagcgtagtg
      961 aggacaaaaa ttcctacttt gtgttgacag ccacaaaaac tccttcaaag acgacgcatt
     1021 gttatttgtg tgagccagac cctacaaaaa aaggccacaa cgataagaac tgcgcagttt
     1081 taatcgcggt tggcgctggt tctcgaccaa ccgcacgatc agtatttgga gtcgcagctc
     1141 cctgtatttt ggcgttgctg catttcactt gaacgttcgc gctgccgcag agcattaagg
     1201 tgaacggaca acaataggtc atgagcaccc cgactacaat gttagacggg gagcatcctg
     1261 atctacataa ttgcagcagc atttccctca attccttcgc ttgcccaagt cgattagtga
     1321 agtatttcaa ttcttcgaga tgattcacat atgcgcgtga agtttcaaag acacgtgtta
     1381 ctcaggtacg ccgtagtgtg tttaacaaat atgaacttct gcactctttg cctgtgctgc
     1441 tctgttactg tataagcatt ttcaacacat cgaccataat tttgcagcgt tgtcagtaat
     1501 gtgacacgag aacgaagttc aagcagacct agaca
//