From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Rhizobium meliloti phosphate regulatory protein (phoU) gene,
Date: 21 Dec 92 20:35:19 GMT
Distribution: bionet
Checksum: 47918 4

LOCUS       RHMPHOB      1371 bp ds-DNA             BCT       19-NOV-1992
DEFINITION  Rhizobium meliloti phosphate regulatory protein (phoU) gene,
            complete cds; phosphate regulatory protein (phoB) gene, complete
            cds.
ACCESSION   M96261
KEYWORDS    phoB gene; phoU gene; phosphate regulatory protein.
SOURCE      Rhizobium meliloti (strain 1021) DNA.
  ORGANISM  Rhizobium meliloti
            Prokaryotae; Gracilicutes; Scotobacteria; Rhizobiaceae.
REFERENCE   1  (bases 1 to 1371)
  AUTHORS   McLean,P.A., Liu,C.-M., Sookdeo,C.C. and Cannon,F.C.
  TITLE     Genetics and phoB regulation of phosphonate utilization in
            Rhizobium meliloti 1021
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     RBS             452..457
                     /gene="phoB"
     stem_loop       1181..1210
                     /function="terminator"
                     /note="putative"
     CDS             <1..356
                     /standard_name="PhoU protein"
                     /gene="phoU"
                     /product="phosphate regulatory protein"
                     /codon_start=3
                     /translation="LARGLEHLAELALVQLKEVLDVYASRSPEKANSIRERDEEIDAI
                     YTSLFRELLTYMMEDPRNITPCTHLLFCAKNIERIGDHATNIAETIYYMATGAQPQGE
                     RPKDDMTSTLGSVTD"
     CDS             467..1150
                     /standard_name="PhoB protein"
                     /gene="phoB"
                     /product="phosphate regulatory protein"
                     /codon_start=1
                     /translation="MLPKIAVVEDEEALSVLLRYNLEAEGFEVDTILRGDEAEIRLQE
                     RLPDLLILDWMLPGVSGIELCRRLRQRPETERLPIIMLTARGEESERVRGLATGADDY
                     VVKPFSTPELMARVKAMLRRAKPEVLSTLLRCGDIELDRETHRVHRRSREVRLGPTEF
                     RLLEFLMSSPGRVFSRSQLLDGVWGHDIYVDERTVDVHVGRLRKALNFSNMPDVIRTV
                     RGAGYSLES"
BASE COUNT      260 a    424 c    443 g    244 t
ORIGIN      
        1 agcttgcgcg cggcctcgaa cacctggccg agctcgcgct cgttcagttg aaggaagtcc
       61 tggacgtcta cgcctcccgt tcgccggaaa aggcgaacag cattcgcgaa cgcgatgaag
      121 agatcgacgc gatctacact tctctgttcc gggaactgct gacctacatg atggaagatc
      181 cgcgcaacat cacgccctgc acgcatcttc ttttctgcgc caagaacatc gagcgtatcg
      241 gcgatcacgc gaccaacatt gccgaaacga tctactacat ggcgacaggc gcccagccgc
      301 agggcgaacg gccgaaggac gacatgacgt cgacattggg ttccgtgacg gactgagatc
      361 gcccggcatc cgggcctatg cagaatttgt tccggccgct ttggcgcggc ctaccaccgt
      421 gcgcggcggc atgacggctg tcgtgcgtag gaaggagtcc tactgaatgt tgccgaagat
      481 tgccgtagtc gaagatgagg aagccctaag cgttctcctg cgctacaatc tcgaggcaga
      541 aggcttcgag gtcgatacga tccttcgcgg cgacgaggcg gagatccgcc tgcaggagcg
      601 cctgcccgac cttctcatcc tcgattggat gctgcccggg gtttccggca tcgagctctg
      661 ccgcaggctg cgccagcggc cggaaacgga gcgcctgccg atcatcatgc tgacggcgcg
      721 cggcgaggag agcgagcgcg tgcggggcct tgccaccggg gccgacgatt acgtcgtcaa
      781 gcccttctcg acgccggaac tgatggcgag ggtcaaggct atgctcaggc gcgccaagcc
      841 cgaggttctg tcgacgctcc tgcgctgcgg cgatatcgag ctcgaccggg agacgcaccg
      901 cgtccaccgc cgcagccgtg aagtgcgcct cggcccgacg gagttccggc tgctggaatt
      961 tctcatgtcc tcaccggggc gcgtcttctc ccgttcacag ctcctggacg gtgtctgggg
     1021 gcacgacatc tatgtcgacg aacggaccgt ggacgtccat gtcggacgtc tgcgcaaggc
     1081 actgaacttc tccaacatgc ccgacgtcat ccggacggtg cgcggcgcgg gctattcgct
     1141 ggagagctga gaccgaaggt gccggcttac ggcatggcaa aaaaaaaggg gcctttccgg
     1201 ccccattttt cgtcgtgcat ccagagcgga atgagcaagg cgcctcagcg catgcgccgg
     1261 cgctcggcag agggctggta ggccagccgg gcatgaaagg tgcaataggg agaggcttcc
     1321 ggggagtcgt tgccgcagaa gtggaactct tccttgagcg ggtcgccgat c
//