From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Streptomyces sp.chitosanase gene, complete cds.
Date: 29 Dec 92 20:58:51 GMT
Distribution: bionet
Checksum: 20769 4

LOCUS       STMCHS       1575 bp ds-DNA             BCT       11-DEC-1992
DEFINITION  Streptomyces sp.chitosanase gene, complete cds.
ACCESSION   L07779
KEYWORDS    chitosanase; chs gene.
SOURCE      Streptomyces sp. (strain N174) DNA.
  ORGANISM  Streptomyces sp.
            Prokaryotae; Firmicutes; Streptomycetaceae.
REFERENCE   1  (bases 1 to 1575)
  AUTHORS   Fink,D., Boucher,I., Denis,F. and Brzezinski,R.
  TITLE     Cloning and expression in Streptomyces lividans of a
            chitosanase-encoding gene from the actinomycete Kitasatosporia N174
            isolated from soil
  JOURNAL   Biotechnol. Lett. 13, 845-850 (1991)
  STANDARD  full automatic
REFERENCE   2  (bases 1 to 1575)
  AUTHORS   Masson,J.-Y., Denis,F. and Brzezinski,R.
  TITLE     Nucleotide sequence of the chitosanase gene from Streptomyces N174
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     RBS             215..219
     mat_peptide     345..1058
                     /function="hydrolytic enzyme"
                     /product="chitosanase"
                     /gene="chs"
                     /evidence="EXPERIMENTAL"
                     /codon_start=1
                     /citation=[1]
     CDS             224..1061
                     /gene="chs"
                     /evidence="EXPERIMENTAL"
                     /citation=[1]
                     /note="precursor"
                     /product="chitosanase"
                     /codon_start=2
                     /translation="MHSQHRTARIALAVVLTAIPASLATAGVGYASTQASTAVKAGAG
                     LDDPHKKEIAMELVSSAENSSLDWKAQYKYIEDIGDGRGYTGGIIGFCSGTGDMLELV
                     QHYTDLEPGNILAKYLPALKKVNGSASHSGLGTPFTKDWATAAKDTVFQQAQNDERDR
                     VYFDPAVSQAKADGLRALGQFAYYDAIVMHGPGNDPTSFGGIRKTAMKKARTPAQGGD
                     ETTYLNAFLDARKAAMLTEAAHDDTSRVDTEQRVFLKAGNLDLNPPLKWKTYGDPYVI
                     NS"
BASE COUNT      259 a    574 c    518 g    224 t
ORIGIN      
        1 ggatccgtcc ggggcggggc ccctctgcgt gtccgggctc ctctgcgtga gccggagtct
       61 gacggtccgt cacttcggtg gcggatcgtt ggcatgcgcg cggtcaggga tttggggcgg
      121 ggcccttgcg gagggccggg agggggcgct tgaatcggtt aggaaagttt cctaactctc
      181 tctgcgggca cccccatgcc cagcgacaga agacggagcg tcatatgcac tcgcagcacc
      241 ggaccgcacg catcgccctg gccgtcgtcc tcaccgcgat acccgcatcg ctcgccaccg
      301 ccggagtcgg ctacgcctcc actcaggcga gcaccgccgt caaggccggt gccggcctcg
      361 acgatcccca caagaaggag atcgcgatgg agctcgtctc cagcgccgag aactcctccc
      421 tcgactggaa ggcccagtac aagtacatcg aggacatcgg tgacggccgc ggctacaccg
      481 gcggcatcat cggcttctgt tccgggaccg gcgacatgct ggaactcgtc cagcactaca
      541 ccgacctgga gcccggcaac atcctcgcca agtacctgcc cgcgctgaag aaggtcaacg
      601 gctcggcctc ccactccggc ctcggcaccc cgttcaccaa ggactgggcg accgccgcca
      661 aggacaccgt cttccagcag gcccagaacg acgagcgcga ccgggtctac ttcgacccgg
      721 ccgtcagcca ggcgaaggcc gacggcctgc gcgcgctggg ccagttcgcc tactacgacg
      781 ccatcgtgat gcacggcccc ggcaacgacc cgaccagctt cggtggcatc cgcaagaccg
      841 ccatgaagaa ggccaggacc cccgcccagg gcggcgacga gaccacctac ctcaacgcct
      901 tcctggacgc ccgcaaggcc gccatgctca ccgaggccgc gcacgacgac accagccgcg
      961 tggacaccga gcagcgggtc ttcctgaagg ccggcaacct cgacctcaac ccgccgctga
     1021 agtggaagac ctacggggac ccgtacgtca tcaacagctg agccggctcg tccccggtgc
     1081 ggcagcgcac caccccgccg caccgggggc acggcccggg atcgatcggc cgccgaggcc
     1141 agctcggacc tggttcgggg ctgtccggcc cgctgcggtg ttctgcggcc tgttccggtc
     1201 tgttccggcc actcgaaaat gtcgggcgga gtgcggcggg tgcccgtagc gtggtccgcc
     1261 atgacgcctc cctcccgtac ccgccccgcc tacgacgccg atgagcgcac gcagttgatc
     1321 gcctggctgg acatgcagcg ggcggtcgtc cactggaagt gcgacggact gtccggcgag
     1381 gacgcccacc gggtggtgat cccgacctcg cccctgatga ctgcggccgg gctggtctcc
     1441 catctgcggt gggtcgagca ctgctggttc gaggtcatgc tgacggggcg ccccgcgacc
     1501 ggaccgcagt tcgacgagag catcgaggac gcggacatgc gggtggaggg cgtcccgctg
     1561 gagcgactgc tgcag
//