From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: S.cerevisiae EMP70 gene for p24a protein
Date: 4 Sep 92 10:07:34 GMT
Distribution: bionet
Checksum: 57874     5

LOCUS       YSCEMP70     2221 bp ds-DNA             PLN       04-SEP-1992
DEFINITION  S.cerevisiae EMP70 gene for p24a protein
ACCESSION   X67316
KEYWORDS    integral membrane protein; p24a protein.
SOURCE      Saccharomyces cerevisiae DNA.
  ORGANISM  Saccharomyces cerevisiae
            Eukaryota; Fungi; Eumycota; Hemiascomycetes; Endomycetales;
            Saccharomycetaceae.
REFERENCE   1  (bases 1 to 2221)
  AUTHORS   Singer- Krueger,B., Krueger,U. and Riezman,H.
  TITLE     Molecular characterization of putative markers for early and late
            endosomes
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
COMMENT     *source: strain=MH 145-4B; *source: cell_line=MH 145-4B; *source:
            clone_library=pSEY8; *source: clone=pSEY-BS6, pSEY BS-5B;
            From EMBL    entry SCEMP70;  dated 29-JUL-1992.
FEATURES             Location/Qualifiers
     CDS             136..2139
                     /product="p24a 70 kDa precursor"
                     /gene="EMP70"
                     /codon_start=1
                     /translation="MIYKMAHVQLLLLYFFVSTVKAFYLPGVAPTTYRENDNIPLLVN
                     HLTPSMNYQHKDEDGNNVSGDKENFLYSYDYYYNRFHFCQPEKVEKQPESLGSVIFGD
                     RIYNSPFQLNMLQEKECESLCKTVIPGDDAKFINKLIKNGFFQNWLIDGLPAAREVYD
                     GRTKTSFYGAGFNLGFVQVTQGTDIEATPKGAETTDKDVELETRNDCNMVKTYELPYF
                     ANHFDIMIEYHDRGEGNYRVVGVIVEPVSIKRSSPGTCETTGSPLMLDEENDNEVYFT
                     YSVKFNESATSWATRWDKYLHVYDPSIQWFSLINFSLVVVLLSSVVIHSLLRALKSDF
                     ARYNELNLDDDFQEDSGWKLNHGDVFRSPSQSLTLSILVGSGVQLFLMVTCSIFFAAL
                     GFLSPSSRGSLATVMFILYALFGFVGSYTSMGIYKFFNGPYWKANLILTPLLVPGAIL
                     LIIIALNFFLMFVHSSGVIPASTLFFMVFLWFLFSIPSSFAGSLIARKRCHWDEHPTK
                     TNQIARQIPFQPWYLKTIPATLIAGIFPFGSIAVELYFIYTSLWFNKIFYMFGFLFFS
                     FLLLTLTSSLVTILITYHSLCLENWKWQWRGFIIGGAGCALYVFIHSILFTKFKLGGF
                     TTIVLYVGYSSVISLLCCLVTGSIGFISSMLFVRKIYSSIKVD"
     mat_peptide     202..885
                     /product="p24a"
BASE COUNT      605 a    407 c    422 g    787 t
ORIGIN
        1 tttgtatctt tttttggtac gttagttaag ttaatttttg gctccttgtc cagcttttcc
       61 ttcatctgct accgttatat tttcagatat taatttagta gcagagagca agtaccgaaa
      121 ggaaaacaaa acagaatgat ttacaaaatg gcacacgtac agcttttgtt gttgtatttt
      181 tttgtgtcaa cagtcaaagc cttctattta ccaggtgtag cgcctaccac ttacagggaa
      241 aatgacaaca tcccattgct cgttaatcat ttaactccat caatgaatta tcaacacaaa
      301 gatgaggacg gtaataacgt ttctggcgat aaagaaaatt tcttgtactc atacgactac
      361 tattacaata gattccattt ttgtcagcct gaaaaagtcg aaaagcaacc tgaatccttg
      421 ggatccgtta tatttggtga tcgaatttat aattcaccat tccaattgaa tatgttacaa
      481 gaaaaagagt gtgaatccct gtgtaagacc gtaattccgg gagatgatgc taaattcatc
      541 aataaattga taaaaaatgg cttcttccaa aactggctta ttgatggatt accggctgca
      601 agagaggttt atgatggcag aactaagact agtttttatg gtgctggctt caaccttggt
      661 tttgtacagg taactcaagg aaccgacatt gaggctactc caaaaggggc agaaaccacc
      721 gataaggatg tggaattgga aacacgtaat gattgtaaca tggtgaaaac ttacgaattg
      781 ccttactttg ccaaccattt cgatattatg attgaatatc acgatcgtgg tgaaggtaac
      841 tatcgcgtag ttggtgttat cgtcgaacca gtttccataa aacgttccag tccaggtaca
      901 tgtgaaacga ctggcagccc attgatgcta gacgaagaaa atgataatga agtttatttt
      961 acttattccg ttaaattcaa tgaatcagcg acttcttggg ccaccaggtg ggacaaatat
     1021 ttacacgtat atgatccttc tattcaatgg ttttctttga taaatttctc cttagttgtt
     1081 gtcttattgt catctgttgt tatccattcg ctattgcgcg cactgaaaag tgatttcgca
     1141 cgttacaacg agttaaattt ggacgacgat tttcaagaag attcaggttg gaaattgaat
     1201 catggtgatg tttttcgttc gccaagtcaa tcgctaaccc tttcaatttt ggttggttca
     1261 ggagttcaat tatttttgat ggtaacttgc agtatcttct tcgctgcatt gggtttccta
     1321 tcacctagtt ctagaggttc cttagccact gttatgttta tcttatatgc attattcggg
     1381 tttgtcggct cttatacatc tatgggcatc tacaaatttt tcaatgggcc atactggaag
     1441 gcaaacttga tattgactcc acttctagtt cctggtgcta ttctgctaat aattattgcg
     1501 ttgaactttt ttttaatgtt tgttcattct tctggtgtca taccagcaag tactctgttt
     1561 tttatggttt ttctatggtt tttgttctca attccatcat catttgctgg ttcattgatt
     1621 gccaggaaaa gatgtcactg ggatgaacat ccaaccaaga caaaccaaat cgcaagacaa
     1681 atacccttcc agccttggta tttaaagact ataccagcta ctttgattgc tggtatcttt
     1741 ccatttggct ccatcgccgt tgagttgtat ttcatataca caagtttatg gtttaataag
     1801 attttttaca tgtttggttt cctctttttc tctttcctcc tattaacttt gacaagttca
     1861 ctcgtcacta ttttgatcac ttatcattcc ttatgcctgg agaactggaa gtggcaatgg
     1921 agaggattca taattggagg tgccggttgt gctttgtatg tgtttattca ttctatttta
     1981 ttcaccaaat ttaagttggg tggattcacg actattgtct tatacgtggg atactcctca
     2041 gtcatttcat tactctgctg tttggtaacg gggtcaattg gctttatcag cagtatgctc
     2101 tttgttagaa aaatctactc atccataaaa gttgactaga ccaacgaaaa agaggtccta
     2161 tacgttgggt tagggcgttt tttatttatt tctctactgt tgatcttttt taactaagaa
     2221 g
//