From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: sigB=putative sigma factor [Anabaena, PCC 7120, Genomic, 1610 nt]
Date: 27 Jan 93 05:56:10 GMT
Distribution: bionet
Checksum: 40496 5

LOCUS       S118034      1610 bp    DNA             BCT       21-JAN-1993
DEFINITION  sigB=putative sigma factor [Anabaena, PCC 7120, Genomic, 1610 nt]
ACCESSION   S48588 M95760
KEYWORDS    .
SOURCE      Anabaena PCC 7120
  ORGANISM  Anabaena
            Unclassified.
REFERENCE   1  (bases 1 to 1610)
  AUTHORS   Brahamsha,B. and Haselkorn,R.
  TITLE     Identification of multiple RNA polymerase sigma factor homologs in
            the cyanobacterium Anabaena sp. strain PCC 7120: cloning,
            expression, and inactivation of the sigB and sigC genes.
  JOURNAL   J. Bacteriol. 174, 7273-7282 (1992)
  STANDARD  full automatic
COMMENT     This entry [NCBI gibbsq 118034] was created by the journal scanning
            component of NCBI/GenBank at the National Library of Medicine.
            This sequence comes from Fig. 3.
FEATURES             Location/Qualifiers
     CDS             2..445
                     /partial
                     /note="Description: orf 5' of sigB;  For the protein
                     sequence (NCBI gibbsq 118035):  Method: conceptual
                     translation supplied by author.  This sequence comes from
                     Fig. 3.  The authors begin their numbering at 537."
                     /codon_start=1
                     /translation="RVEAAVKLYKMGKISKLLMTGDNSTVSYNEVRSMLKYAHDLGVP
                     MKNITLDYAGFSTYESCYRAHKVFGVHKAVVITQNYHLPRTVYTCRQLGLKTVGLGTP
                     DIEIYGLRGMIPDLLRESLANVKALWEVDITRPRPTFLGQFEPIN"
     CDS             537..1535
                     /note="putative sigma factor;  For the protein sequence
                     (NCBI gibbsq 118036):  Method: conceptual translation
                     supplied by author.  This sequence comes from Fig. 3."
                     /gene="sigB"
                     /product="SigB"
                     /codon_start=1
                     /translation="MPNSTSQVTKLKSKNNEFSYTADAVSIYLHKIGRVPLLSHEQEI
                     FFAQQVQQMMVMFTAKEELAEKLQREPTLQEWADKMQLKEDVLLQQLSQGQIAKQKMI
                     QANLRLVVSIAKKYQKRNLEFLDLIQEGALGLERGVEKFDPTLGYKFSTYAYWWIRQG
                     ITRAIAQQSRTIRLPIHMADKLNKIKCVQRELSQKLGYIAGVTEIAQALNLEPSQIRE
                     YLQLVRQPVSLDMRIGFEQDTQLQDLLKDDGMSPERYAERELLYQDIHNLLAKLTPQQ
                     KEVLILRFGLAGGCELTLVQISQRMGISRERVRQVEKQALTLLRRYGIDSRSYLAD"
BASE COUNT      503 a    302 c    338 g    467 t
ORIGIN      
        1 ccgtgtagaa gctgctgtca aactttataa aatgggaaag attagtaaac tcttgatgac
       61 aggagataac agtacagttt cctataatga agtcaggtct atgctgaaat atgcccatga
      121 tctgggtgta ccgatgaaaa acattaccct agactatgca ggtttcagca cttatgagag
      181 ttgttatcgc gctcataaag tttttggtgt acataaagct gttgtcataa ctcaaaatta
      241 tcatcttccc cgcactgttt acacctgtcg ccaattagga ctaaagacag ttggtttagg
      301 aactccagat atagaaattt atggactacg aggaatgatt cctgatttat tacgggaatc
      361 attagcgaat gttaaggctt tgtgggaagt tgacattacc cgtccccgac ctactttttt
      421 aggacagttt gaaccaatta attgatgaag tttttaaaat ttatcatttc gattatttta
      481 tgcttattta tttttatgta accatttgat tttattcaat tgaggctaac atatttatgc
      541 ctaattcaac atcccaagtg actaaactta agagcaagaa taacgaattt tcatatacag
      601 cagatgcagt aagtatctat cttcataaaa ttggacgtgt acctctgtta agccatgagc
      661 aagaaatttt ttttgctcaa caagttcagc aaatgatggt gatgtttact gcaaaagaag
      721 agctagctga aaaattacag cgtgaaccga ctctgcaaga atgggctgac aagatgcagt
      781 tgaaagaaga tgtgctgctg caacaactaa gtcaaggaca aattgccaag cagaagatga
      841 ttcaggctaa tctgcggtta gtggtatcta ttgctaaaaa ataccagaaa cgcaatctgg
      901 agtttctaga cttaattcaa gaaggtgcat tggggctaga acgaggggta gagaaatttg
      961 atccaactct tggatacaag ttttccactt atgcttactg gtggattcgt cagggaatta
     1021 cacgagcaat agcacaacaa tcccgcacta ttcgtttacc cattcacatg gctgataagc
     1081 tgaacaaaat taagtgtgtt cagcgagagt tatctcaaaa gcttggttac attgctggcg
     1141 taacggaaat cgcccaagcc ctcaatttgg aacctagtca gattcgagaa tatttgcagc
     1201 ttgttcgtca acctgtttcc ttagatatgc gaattgggtt tgagcaggat acccaattac
     1261 aggatctgct gaaggatgat ggaatgtctc ccgaacgcta cgccgaacga gaattgctct
     1321 atcaagacat tcacaaccta ttagcaaagc tgactcccca gcaaaaagaa gtcttaatct
     1381 tgcgctttgg tttagcaggt ggatgcgaac tgaccctagt acagattagt caacggatgg
     1441 gtattagtcg ggaacgagta cgacaagtgg aaaaacaagc tctcacgctt ctacgaagat
     1501 atggaattga ctcacgcagc tatctagctg actgatactc tttaattttg aaagcttgta
     1561 gtgtcaactg gtaagtgaaa aaccgcctgc ttgctgtcac tggtctaaat
//