From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Aix sp. mRNA for malic enzyme
Date: 21 Jul 92 10:03:25 GMT
Distribution: bionet
Checksum: 59601     5

LOCUS       AIXPMAL      1674 bp ss-RNA             VRT       21-JUL-1992
DEFINITION  Aix sp. mRNA for malic enzyme
ACCESSION   X66418
KEYWORDS    malic enzyme.
SOURCE      Aix sp. RNA.
  ORGANISM  Aix sp.
            Eukaryota; Animalia; Chordata; Vertebrata; Aves; Neornithes;
            Neognathae; Anseriformes; Anatidae.
REFERENCE   1  (bases 1 to 1674)
  AUTHORS   Hsu,R.Y., Glynias,M.J., Satterlee,J.D., Feeney,R., Clarke,A.R.,
            Emery,D.S., Roe,B.A., Wilson,R.K., Goodrindge,A.G. and
            Holbrook,J.J.
  TITLE     Duck liver malic enzyme. Expression in Escherichia coli and
            characterization of the wild-type enzyme and site-directed mutation
  JOURNAL   Biochem. J. (1992) In press
  STANDARD  full automatic
COMMENT     *source: stage=1-3 week old duckling; *source: tissue=liver;
            *source: cell_line=E.coli TG-2 cells; *source: clone_library=
            pBR322; *source: clone=M13;
            From EMBL    entry ASPMAL;  dated 10-JUN-1992.
FEATURES             Location/Qualifiers
     CDS             1..1674
                     /EC_number="1.1.1.40"
                     /product="malatedehydrogenase (oxaloacetate
                     decarboxylating)(NADP+)"
                     /codon_start=1
                     /translation="MKRGYEVLRDPHLNKGMAFTLEERQQLNIHGLLPPCFLGQDVQV
                     FSILKNFERLTSDLDRYILLMSLQDRNEKLFYKVLTSDIERFMPIVYTPTVGLACQQY
                     GLAFRRPRGLFITIHDRGHIATMLKSWPESVIKAIVVTDGERILGLGDLGCYGMGIPV
                     GKLALYTACGGVKPHECLPVMLDVGTDNEALLKDPLYIGLRHKRIRGQAYDDLLDEFM
                     EAVTSRYGMNCLIQFEDFANANAFRLLHKYRNKYCTFNDDIQGTASVAVAGLLAALRI
                     TKNRLSDHTVLFQGAGEAALGIANLIVMAMEKEGVSKEAAVKRIWMVDSKGLIVKGRA
                     SLTAEKTRFAHEHAEMKNLEDIVKDIKPSVLIGVAAIGGAFTKEILQDMAAFNKRPII
                     FALSNPTSKAECTAEQCYKYTEGRGIFASGSPFDPVTLPNGKTLYPGQGNNSYVFPGV
                     ALGVIACGLKHIGEDVFLTTAEVIAEQVSEENLQEGRLYPPLVTIQHVSLKIAVRIAE
                     EAYRNNTASTYPQPKDLEAFIQSQIYSTDYNSFVADSYTWPEEAMKVKL"
BASE COUNT      469 a    353 c    406 g    446 t
ORIGIN
        1 atgaagaggg gctacgaggt gctccgcgac cctcacctca acaagggaat ggcatttacc
       61 ttggaggaga ggcaacaact gaatattcat ggactattgc caccttgctt tctcggtcaa
      121 gatgtccagg ttttcagtat tcttaagaat tttgaaagac tgacatccga cctagacaga
      181 tacattctac taatgagtct tcaggatcga aatgaaaagc tcttctataa agtgctgact
      241 tctgacatag aaaggttcat gcctattgtt tatactccca ctgtgggact ggcttgccag
      301 caatatggtt tagcatttcg gaggccaaga ggacttttta ttactatcca tgaccgggga
      361 cacatcgcta caatgctcaa gtcctggcca gaaagtgtca tcaaggctat tgtggtgaca
      421 gatggagaac gtattcttgg tcttggagac ctcggctgct atggcatggg catcccggtt
      481 ggtaaactgg cactttatac cgcgtgtgga ggagtgaaac ctcatgagtg cctaccagtg
      541 atgttggatg tgggaacaga taatgaggca ctgctgaaag atcctttgta tattgggctg
      601 agacacaaga gaatcagagg tcaagcctat gatgatctcc ttgatgaatt catggaagct
      661 gtgacttcaa ggtatggcat gaactgcctc attcaatttg aggattttgc taatgccaat
      721 gcattccgcc tcctgcataa gtaccgtaac aagtattgca ctttcaatga tgacattcaa
      781 ggaactgcat ctgtggctgt tgcaggtctt cttgctgctc tgcgtatcac taagaacagg
      841 ctatcagatc acacagtgct gttccagggg gctggagaag ctgcgctggg gatagcaaac
      901 ctcattgtca tggctatgga aaaagaagga gtatctaaag aggcagctgt caaaagaata
      961 tggatggttg actcaaaggg cttgatagtg aaggggcgtg cctcattgac agctgagaaa
     1021 acgcgatttg cacatgaaca tgctgaaatg aaaaatctag aagatattgt taaagatata
     1081 aaaccatctg tgttaatagg agttgctgca attggtggtg cttttactaa agagattctt
     1141 caggatatgg ctgctttcaa caaacgtcct attatttttg ctctcagcaa tcctaccagc
     1201 aaagcagagt gcactgctga gcagtgctac aaatacacag agggacgtgg tatttttgcc
     1261 agtggaagtc cttttgatcc tgtcactctt ccaaacggaa aaactctgta tcctggacag
     1321 ggtaacaatt cctatgtgtt cccaggagtt gcccttggtg ttattgcatg cggtctgaaa
     1381 catatcggcg aggatgtttt cctcacaact gctgaggtaa tagctgagca agtttcagaa
     1441 gagaatctac aggaaggtcg cctgtaccct cctttagtca caattcagca cgtgtcactg
     1501 aaaatcgctg tgaggatcgc agaggaagcc tacaggaata acactgcctc cacctatcct
     1561 cagcccaagg acctagaagc cttcatccag tctcagattt acagcactga ttataacagc
     1621 ttcgtggctg actcatatac ctggcctgaa gaagcgatga aagtgaaact gtaa
//