From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: E.coli cold shock protein 7.4 (cspA) gene, complete cds.
Date: 29 Jul 92 10:01:43 GMT
Distribution: bionet
Checksum: 33528     4

LOCUS       ECOCSPAA     1205 bp ds-DNA             BCT       29-JUL-1992
DEFINITION  E.coli cold shock protein 7.4 (cspA) gene, complete cds.
ACCESSION   M30139
KEYWORDS    cold shock protein; cspA gene.
SOURCE      E.coli (strain SB221) DNA.
  ORGANISM  Escherichia coli
            Prokaryotae; Gram-negative facultatively anaerobic rods;
            Enterobacteriaceae.
REFERENCE   1  (bases 1 to 1205)
  AUTHORS   Goldstein,J., Pollitt,N.S. and Inouye,M.
  TITLE     Major cold shock protein of Escherichia coli
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 87, 283-287 (1990)
  STANDARD  full staff_review
REFERENCE   2  (sites)
  AUTHORS   Tanabe,H., Goldstein,J., Yang,M. and Inouye,M.
  TITLE     Identification of the promoter region of the Escherichia coli major
            cold shock gene, cspA
  JOURNAL   J. Bacteriol. 174, 3867-3873 (1992)
  STANDARD  full staff_review
COMMENT     Draft entry and computer-readable sequence for [1] kindly submitted
            by J.Goldstein, 21-NOV-1989.
FEATURES             Location/Qualifiers
     misc_signal     329..334
                     /note="promoter sequence (put.)"
                     /note="putative"
     misc_signal     353..358
                     /note="promoter sequence (put.)"
                     /note="putative"
     -35_signal      421..426
     -10_signal      444..449
     mRNA            457..857
                     /note="cspA mRNA (alt.; 3' end approx.)"
     mRNA            458..857
                     /note="cspA mRNA (alt.; 3' end approx.)"
     mRNA            508..857
                     /note="cspA mRNA (alt.; 3' end approx.)"
     RBS             605..607
                     /note="ribosome binding site"
     CDS             617..829
                     /note="cold shock protein (cspA)"
                     /codon_start=1
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     misc_signal     857..878
                     /note="cspA transcription termination signal"
BASE COUNT      373 a    255 c    251 g    326 t
ORIGIN      1 bp upstream of HindIII site; 79 min. on K12 map.
        1 aagcttcgat gcaattcacg atcccgcagt gtgatttgag gagttttcaa tggaatataa
       61 agatccaatg catgagctgt tgagcagcct ggaacagatt gtttttaaag atgaaacgca
      121 gaaaattacc ctgacgcaca gaacaacgtc ctgtaccgaa attgagcagt tacgaaaagg
      181 gacaggatta aaaatcgatg atttcgcccg ggttttgggc gtatcagtcg ccatggtaaa
      241 ggaatgggaa tccagacgcg tgaagccttc aagtgccgaa ctaaaattga tgcgtttgat
      301 tcaagccaac ccggcattaa gtaagcagtt gatggaatag actttatcca cttatgctgt
      361 ttacggtcct gatgacagac cgttttccaa ccgattaatc ataaatatga aaaataattg
      421 ttgcatcacc cgccaatgcg tggcttaatg cacatcaacg gtttgacgta cagaccatta
      481 aagcagtgta gtaaggcaag tcccttcaag agttatcgtt gatacccctc gtagtgcaca
      541 ttcctttaac gcttcaaaat ctgtaaagca cgccatatcg ccgaaaggca cacttaatta
      601 ttaaaggtaa tacactatgt ccggtaaaat gactggtatc gtaaaatggt tcaacgctga
      661 caaaggcttc ggcttcatca ctcctgacga tggctctaaa gatgtgttcg tacacttctc
      721 tgctatccag aacgatggtt acaaatctct ggacgaaggt cagaaagtgt ccttcaccat
      781 cgaaagcggc gctaaaggcc cggcagctgg taacgtaacc agcctgtaat ctctgcttaa
      841 aagcacagaa tctaagatcc ctgccatttg gcggggattt ttttatttgt tttcaggaaa
      901 taaataatcg atcgcgtaat aaaatctatt attatttttg tgaagaataa atttgggtgc
      961 aatgagaatg cgcaacgccg taagtaaggc gggaataatt tcccgccgaa gactcttact
     1021 ctttcaattt gcaggctaaa aacgccgcca gctcataact ctcctgttta atatgcaatt
     1081 cacacagtga atctcttatc atccaggtga aaaataaaag cgtgaaacaa atcactatta
     1141 aagaaagtaa tctatatttc tgcgcattcc agctctgtgt tgatttcacg agtatgtact
     1201 gcacc
//