From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Bacillus thuringiensis delta-endotoxin (CryA(a)) gene, 5' end.
Date: 22 Dec 92 22:50:35 GMT
Distribution: bionet
Checksum: 38074 9

LOCUS       BACDRYVAA    4155 bp ds-DNA             BCT       30-NOV-1992
DEFINITION  Bacillus thuringiensis delta-endotoxin (CryA(a)) gene, 5' end.
ACCESSION   L07025
KEYWORDS    delta-endotoxin.
SOURCE      Bacillus thuringiensis (individual_isolate PS17, serotype 10,
            sub_species darmstadiensis) DNA.
  ORGANISM  Bacillus thuringiensis
            Prokaryotae; Firmicutes; Endospore-forming rods and cocci;
            Bacillaceae.
REFERENCE   1  (bases 1 to 4155)
  AUTHORS   Narva,K.E., Payne,J.M., Schwab,G.E., Hickle,L.A., Galasan,T. and
            Sick,A.J.
  TITLE     Novel Bacillus thuringiensis microbes active against nematodes, and
            genes encoding novel nematode-active toxins cloned from Bacillus
            thuringiensis isolates
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     CDS             1..>4155
                     /gene="CryVA"
                     /product="delta-endotoxin"
                     /codon_start=1
                     /translation="MAILNELYPSVPYNVLAYTPPSFLPDAGTQATPADLTAYEQLLK
                     NLEKGINAGTYSKAIADVLKGIFIDDTINYQTYVNIGLSLITLAVPEIGIFTPFIGLF
                     FAALNKHDAPPPPNAKDIFEAMKPAIQEMIDRTLTADEQTFLNGEISGLQNLAARYQS
                     TMDDIQSHGGFNKVDSGLIKKFTDEVLSLNSFYTDRLPVFITDNTADRTLLGLPYYAI
                     LASMHLMLLRDIITKGPTWDSKINFTPDAIDSFKTDIKNNIKLYSKTIYDVFQKGLAS
                     YGTPSDLESFAKKQKYIEIMTTHCLDFARLFPTFDPDLYPTGSGDISLQKTRRILSPF
                     IPIRTADGLTLNNTSIDTSNWPNYENGNGAFPNPKERILKQFKLYPSWRAGQYGGLLQ
                     PYLWAIEVQDSVETRLYGQLPAVDPQAGPNYVSIDSSNPIIQINMDTWKTPPQGASGW
                     NTNLMRGSVSGLSFLQRDGTRLSAGMGGGFADTIYSLPATHYLSYLYGTPYQTSDNYS
                     GHVGALVGVSTPQEATLPNIIGQPDEQGNVSTMGFPFEKASYGGTVVKEWLNGANAMK
                     LSPGQSIGIPITNVTSGEYQIRCRYASNDNTNVFFNVDTGGANPIFQQINFASTVDNN
                     TGVQGANGVYVVKSIATTDNSFTEIPAKTINVHLTNQGSSDVFLDRIEFIPFSLPLIY
                     HGSYNTSSGADDVLWSSSNMNYYDIIVNGQANSSSIASSMHLLNKGKVIKTIDIPGHS
                     ETFFATFPVPEGFNEVRILAGLPEVSGNITVQSNNPPQPSNNGGGDGGGNGGGDGGQY
                     NFSLSGSDHTTIYHGKLETGIHVQGNYTYTGTPVLILNAYRNNTVVSSIPVYSPFDIT
                     IQTEADSLELELQPRYGFATVNGTATVKSPNVNYDRSFKLPIDLQNITTQVNALFASG
                     TQNMLAHNVSDHDIEEVVLKVDALSDEVFGDEKKALRKLVNQAKRLSRARNLLIGGSF
                     ENWDAWYKGRNVVTVSDHELFKSDHVLLPPPGLSPSYIFQKVEESKLKPNTRYIVSGF
                     IAHGKDLEIVVSRYGQEVQKVVQVPYGEAFPLTSNGPVCCPPRSTSNGTLGDPHFFSY
                     SIDVGALDLQANPGIEFGLRIVNPTGMARVSNLEIREDRPLAANEIRQVQRVARNWRT
                     EYEKERAEVTSLIQPVINRINGLYENGNWNGSIRSDISYQNIDAIVLPTLPKLRHWFM
                     SDRFSEQGDIMAKFQGALNRAYAQLEQSTLLHNGHFTKDAANWTIEGDAHQITLEDGR
                     RVLRLPDWSSSVSQMIEIENFNPDKEYNLVFHGQGEGTVTLEHGEETKYIETHTHHFA
                     NFTTSQRQGLTFESNKVTVTISSEDGEFLVDNIALVEAPLPTDDQNSEGNTASSTNSD
                     TSMNNNQ"
BASE COUNT     1386 a    677 c    811 g   1281 t
ORIGIN      
        1 atggcaattt taaatgaatt atatccatct gtaccttata atgtattggc gtatacgcca
       61 ccctcttttt tacctgatgc gggtacacaa gctacacctg ctgacttaac agcttatgaa
      121 caattgttga aaaatttaga aaaagggata aatgctggaa cttattcgaa agcaatagct
      181 gatgtactta aaggtatttt tatagatgat acaataaatt atcaaacata tgtaaatatt
      241 ggtttaagtt taattacatt agctgtaccg gaaattggta tttttacacc tttcatcggt
      301 ttgttttttg ctgcattgaa taaacatgat gctccacctc ctcctaatgc aaaagatata
      361 tttgaggcta tgaaaccagc gattcaagag atgattgata gaactttaac tgcggatgag
      421 caaacatttt taaatgggga aataagtggt ttacaaaatt tagcagcaag ataccagtct
      481 acaatggatg atattcaaag ccatggagga tttaataagg tagattctgg attaattaaa
      541 aagtttacag atgaggtact atctttaaat agtttttata cagatcgttt acctgtattt
      601 attacagata atacagcgga tcgaactttg ttaggtcttc cttattatgc tatacttgcg
      661 agcatgcatc ttatgttatt aagagatatc attactaagg gtccgacatg ggattctaaa
      721 attaatttca caccagatgc aattgattcc tttaaaaccg atattaaaaa taatataaag
      781 ctttactcta aaactattta tgacgtattt cagaagggac ttgcttcata cggaacgcct
      841 tctgatttag agtcctttgc aaaaaaacaa aaatatattg aaattatgac aacacattgt
      901 ttagattttg caagattgtt tcctactttt gatccagatc tttatccaac aggatcaggt
      961 gatataagtt tacaaaaaac acgtagaatt ctttctcctt ttatccctat acgtactgca
     1021 gatgggttaa cattaaataa tacttcaatt gatacttcaa attggcctaa ttatgaaaat
     1081 gggaatggcg cgtttccaaa cccaaaagaa agaatattaa aacaattcaa actgtatcct
     1141 agttggagag cgggacagta cggtgggctt ttacaacctt atttatgggc aatagaagtc
     1201 caagattctg tagagactcg tttgtatggg cagcttccag ctgtagatcc acaggcaggg
     1261 cctaattatg tttccataga ttcttctaat ccaatcatac aaataaatat ggatacttgg
     1321 aaaacaccac cacaaggtgc gagtgggtgg aatacaaatt taatgagagg aagtgtaagc
     1381 gggttaagtt ttttacaacg agatggtacg agacttagtg ctggtatggg tggtggtttt
     1441 gctgatacaa tatatagtct ccctgcaact cattatcttt cttatctcta tggaactcct
     1501 tatcaaactt ctgataacta ttctggtcac gttggtgcat tggtaggtgt gagtacgcct
     1561 caagaggcta ctcttcctaa tattataggt caaccagatg aacagggaaa tgtatctaca
     1621 atgggatttc cgtttgaaaa agcttcttat ggaggtacag ttgttaaaga atggttaaat
     1681 ggtgcgaatg cgatgaagct ttctcctggg caatctatag gtattcctat tacaaatgta
     1741 acaagtggag aatatcaaat tcgttgtcgt tatgcaagta atgataatac taacgttttc
     1801 tttaatgtag atactggtgg agcaaatcca attttccaac agataaactt tgcatctact
     1861 gtagataata atacgggagt acaaggagca aatggtgtct atgtagtcaa atctattgct
     1921 acaactgata attcttttac agaaattcct gcgaagacga ttaatgttca tttaaccaac
     1981 caaggttctt ctgatgtctt tttagaccgt attgaattta tacctttttc tctacctctt
     2041 atatatcatg gaagttataa tacttcatca ggtgcagatg atgttttatg gtcttcttca
     2101 aatatgaatt actacgatat aatagtaaat ggtcaggcca atagtagtag tatcgctagt
     2161 tctatgcatt tgcttaataa aggaaaagtg ataaaaacaa ttgatattcc agggcattcg
     2221 gaaaccttct ttgctacgtt cccagttcca gaaggattta atgaagttag aattcttgct
     2281 ggccttccag aagttagtgg aaatattacc gtacaatcta ataatccgcc tcaacctagt
     2341 aataatggtg gtggtgatgg tggtggtaat ggtggtggtg atggtggtca atacaatttt
     2401 tctttaagcg gatctgatca tacgactatt tatcatggaa aacttgaaac tgggattcat
     2461 gtacaaggta attataccta tacaggtact cccgtattaa tactgaatgc ttacagaaat
     2521 aatactgtag tatcaagcat tccagtatat tctccttttg atataactat acagacagaa
     2581 gctgatagcc ttgagcttga actacaacct agatatggtt ttgccacagt gaatggtact
     2641 gcaacagtaa aaagtcctaa tgtaaattac gatagatcat ttaaactccc aatagactta
     2701 caaaatatca caacacaagt aaatgcatta ttcgcatctg gaacacaaaa tatgcttgct
     2761 cataatgtaa gtgatcatga tattgaagaa gttgtattaa aagtggatgc cttatcagat
     2821 gaagtatttg gagatgagaa gaaggcttta cgtaaattgg tgaatcaagc aaaacgtttg
     2881 agtagagcaa gaaatcttct gataggtggg agttttgaaa attgggatgc atggtataaa
     2941 ggaagaaatg tagtaactgt atctgatcat gaactattta agagtgatca tgtattatta
     3001 ccaccaccag gattgtctcc atcttatatt ttccaaaaag tggaggaatc taaattaaaa
     3061 ccaaatacac gttatattgt ttctggattc atcgcacatg gaaaagacct agaaattgtt
     3121 gtttcacgtt atgggcaaga agtgcaaaag gtcgtgcaag ttccttatgg agaagcattc
     3181 ccgttaacat caaatggacc agtttgttgt cccccacgtt ctacaagtaa tggaacctta
     3241 ggagatccac atttctttag ttacagtatc gatgtaggtg cactagattt acaagcaaac
     3301 cctggtattg aatttggtct tcgtattgta aatccaactg gaatggcacg cgtaagcaat
     3361 ttggaaattc gtgaagatcg tccattagca gcaaatgaaa tacgacaagt acaacgtgtc
     3421 gcaagaaatt ggagaaccga gtatgagaaa gaacgtgcgg aagtaacaag tttaattcaa
     3481 cctgttatca atcgaatcaa cggattgtat gaaaatggaa attggaacgg ttctattcgt
     3541 tcagatattt cgtatcagaa tatagacgcg attgtattac caacgttacc aaagttacgc
     3601 cattggttta tgtcagatag attcagtgaa caaggagata taatggctaa attccaaggt
     3661 gcattaaatc gtgcgtatgc acaactggaa caaagtacgc ttctgcataa tggtcatttt
     3721 acaaaagatg cagctaattg gacaatagaa ggcgatgcac atcagataac actagaagat
     3781 ggtagacgtg tattgcgact tccagattgg tcttcgagtg tatctcaaat gattgaaatc
     3841 gagaatttta atccagataa agaatacaac ttagtattcc atgggcaagg agaaggaacg
     3901 gttacgttgg agcatggaga agaaacaaaa tatatagaaa cgcatacaca tcattttgcg
     3961 aattttacaa cttctcaacg tcaaggactc acgtttgaat caaataaagt gacagtgacc
     4021 atttcttcag aagatggaga attcttagtg gataatattg cgcttgtgga agctcctctt
     4081 cctacagatg accaaaattc tgagggaaat acggcttcca gtacgaatag cgatacaagt
     4141 atgaacaaca atcaa
//