From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Propionibacterium shermanii (clone pLac5s) methylmalonyl-CoA
Date: 21 Dec 92 20:34:50 GMT
Distribution: bionet
Checksum: 12039 5

LOCUS       PRSMMCOACT   1560 bp ds-DNA             BCT       18-NOV-1992
DEFINITION  Propionibacterium shermanii (clone pLac5s) methylmalonyl-CoA
            carboxyltransferase subunit 5S monomer gene, complete cds.
ACCESSION   L06488
KEYWORDS    methylmalonyl-CoA carboxyltransferase subunit 5S monomer;
            transcarboxylase.
SOURCE      Propionibacterium shermanii (strain ST33) (library: pPS-lib) DNA.
  ORGANISM  Propionibacterium shermanii
            Prokaryotae; Firmicutes; Irregular asporogenous rods.
REFERENCE   1  (bases 1 to 1560)
  AUTHORS   Thornton,C.G., Kumar,G.K., Shenoy,B.C., Haase,C.F., Park,V.M.,
            Magner,W.J., Hejlik,D.P., Wood,H.G. and Samols,D.
  TITLE     Primary structure of the 5 S subunit of transcarboxylase as deduced
            from the genomic DNA sequence
  JOURNAL   Unpublished (1993)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     CDS             1..1560
                     /function="catalyzes carboxyl transfer to pyruvate"
                     /EC_number="2.1.3.1"
                     /standard_name="transcarboxylase"
                     /evidence="EXPERIMENTAL"
                     /product="methylmalonyl-CoA carboxyltransferase 5S subunit
                     monomer"
                     /codon_start=1
                     /translation="MSPREIEVSEPREVGITELVLRDAHQSLMATRMAMEDMVGACAD
                     IDAAGYWSVECWGGATYDSCIRFLNEDPWERLRTFRKLMPNSRLQMLLRGQNLLGYRH
                     YNDEVVDRFVDKSAENGMDVFRVFDAMNDPRNMAHAMAAVKKAGKHAQGTICYTISPV
                     HTVEGYVKLAGQLLDMGADSIALKDMAALLKPQPAYDIIKAIKDIRPEDADQPALHST
                     TGVTEVSLMKAIEAGVDTAISSMSLGPGHNPTESVAEMLEGTGYTTNLDYDRLHKIRD
                     HFKAIRPKYKKFESKTLVDTSIFKSQIPGGMLSNMESQLRAQGAEDKMDEVMAEVPRV
                     RRPAPVFPAPGHPVQPDRRHAGLFNVMMGEYKRMTGEFADIMLGYYGATPADRDPKWS
                     VGEEHRRAITQRPADHDPKVVKLAEEQSGKKPITQRPADLLPPEWEEQSKEPRPKGFN
                     GTDEDVLTYALFPQVAPVFFESRPRAAEVALTDAQLKAEARATRSVAVAGPVTYNVNV
                     RNRPQVTVQQA"
BASE COUNT      316 a    501 c    480 g    263 t
ORIGIN      
        1 atgagtccgc gagaaattga ggtttccgag ccgcgcgagg ttggtatcac cgagctcgtg
       61 ctgcgcgatg cccatcagag cctgatggcc acacgaatgg caatggaaga catggtcggc
      121 gcctgtgcag acattgatgc tgccgggtac tggtcagtgg agtgttgggg tggtgccacg
      181 tatgactcgt gtatccgctt cctcaacgag gatccttggg agcgtctgcg cacgttccgc
      241 aagctgatgc ccaacagccg tctccagatg ctgctgcgtg gccagaacct gctgggttac
      301 cgccactaca acgacgaggt cgtcgatcgt ttcgtcgaca agtccgctga gaacggcatg
      361 gacgtgttcc gtgtcttcga cgccatgaat gatccccgca atatggcgca cgccatggct
      421 gccgtcaaga aggccggcaa gcacgcgcag ggcaccattt gctacacgat cagcccggtc
      481 cacaccgttg agggctatgt caagcttgct ggtcagctgc tcgacatggg tgctgattcc
      541 atcgccctga aggacatggc cgccctgctc aagccgcagc cggcctacga catcatcaag
      601 gccatcaagg acatacggcc agaagacgca gatcaacctg cactgcactc caccacgggt
      661 gtcaccgagg tctccctcat gaaggccatc gaggccggcg tcgacaccgc catctcgtcc
      721 atgtcgctcg gcccgggcca caaccccacc gagtcggttg ccgagatgct cgagggcacc
      781 gggtacacca ccaaccttga ctacgatcgc ctgcacaaga tccgcgatca cttcaaggcc
      841 atccgcccga agtacaagaa gttcgagtcg aagacgcttg tcgacacctc gatcttcaag
      901 tcgcagatcc ccggcggcat gctgtccaac atggagtcgc agctgcgcgc ccagggcgcc
      961 gaggacaaga tggacgaggt catggcagag gtgccgcgcg tccgaaggcc ggcgccggtt
     1021 ttccccgccc ctggtcaccc cgtccagcca gatcgtcggc acgcaggcct gttcaacgtg
     1081 atgatgggcg agtacaagag gatgaccggc gagttcgcag atatcatgct cggctactac
     1141 ggcgccacgc cggccgatcg cgatccgaag tggtcagttg gcgaggagca tcgcagagcg
     1201 atcacccagc gcccggccga tcacgatccg aaggtggtca agttggccga ggagcagtcc
     1261 ggcaagaagc cgatcaccca gcgcccggcc gatctgctgc cccccgagtg ggaggagcag
     1321 tccaaggagc cgcgccctaa gggcttcaac ggcaccgacg aggacgtgct cacctatgca
     1381 ctgttcccgc aggtcgctcc ggtcttcttc gagagtcggc cgagggccgc agaggtggct
     1441 ctcaccgatg cccagctgaa ggccgaggcg agggcgacga gaagtgtcgc cgtggccggt
     1501 cccgtcacct acaacgtgaa cgtgcggaac cgtccgcaag tcaccgttca gcaggcgtga
//