From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: E.coli proA and ret genes encoding gamma-glutamylphosphate
Date: 25 Jul 92 10:03:14 GMT
Distribution: bionet
Checksum: 32087     9

LOCUS       ECOPRORET    3440 bp ds-DNA             BCT       25-JUL-1992
DEFINITION  E.coli proA and ret genes encoding gamma-glutamylphosphate
            reductase and reverse transcriptase
ACCESSION   Z12832
KEYWORDS    ATP binding protein; gamma-glutamyl phosphate reductase; proA gene;
            ret gene; reverse transcriptase; thrW gene.
SOURCE      Escherichia coli DNA.
  ORGANISM  Escherichia coli
            Prokaryotae; Gram-negative facultatively anaerobic rods;
            Enterobacteriaceae.
REFERENCE   1  (bases 1 to 3440)
  AUTHORS   Lim,D.
  TITLE     Structure and biosynthesis of unbranched multicopy single-stranded
            DNA by reverse transcriptase in a clinical E.coli isolate
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
REFERENCE   2  (bases 1 to 3440)
  AUTHORS   Lim,D.
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
COMMENT     *source: strain=Clinical strain; *source: clone=pDB32; *source:
            isolate=161; *source: is_macronuclear=N; *source: is_proviral=N;
            *source: is_germline=N;
            From EMBL    entry ECPRORET;  dated 26-JUN-1992.
FEATURES             Location/Qualifiers
     CDS             <1..153
                     /function="proline biosynthesis"
                     /product="gamma-glutamylphosphatereductase"
                     /gene="proA"
                     /codon_start=1
                     /translation="ASTRFTDGGQFGLGAEVAVSTQKLHARGPMGLEALTTYKWIGIG
                     DYTIRA"
     tRNA            268..343
                     /note="atachment site of phage P22"
                     /gene="thrW"
     repeat_unit     542..549
                     /rpt_type=inverted
     misc_RNA        542..624
                     /note="guanine atposition 550 is the branched residue"
                     /product="msdRNA"
                     /gene="msr"
     misc_feature    complement(616..694)
                     /note="final product ofreverse transcription"
                     /product="unbranched mature msDNA;
                     multicopysingle-stranded DNA"
     misc_feature    complement(616..698)
                     /note="precursor forunbranched msDNA"
                     /product="branched msDNA"
     repeat_unit     700..707
                     /rpt_type=inverted
     CDS             794..1732
                     /function="branched msDNA-RNA biosynthesis"
                     /note="bacterial reverse transcriptaseencoded by retron"
                     /product="reverse transcriptase"
                     /gene="ret"
                     /codon_start=1
                     /translation="MSIDIETTLQKAYPDFDVLLKSRPATHYKVYKIPKRTIGYRIIA
                     QPTPRVKAIQRDIIEILKQHTHIHDAATAYVDGKNILDNAKIHQSSVYLLKLDLVNFF
                     NKITPELLFKALARQKVDISDTNKNLLKQFCFWNRTKRKNGALVLSVGAPSSPFISNI
                     VMSSFDEEISSFCKENKISYSRYADDLTFSTNERDVLGLAHQKVKTTLIRFFGTRIII
                     NNNKIVYSSKAHNRHVTGVTLTNNNKLSLGRERKRYITSLVFKFKEGKLSNVDINHLR
                     GLIGFAYNIEPAFIERLEKKYGESTIKSIKKYSEGG"
     CDS             1733..3211
                     /product="hypothetical ATP binding protein"
                     /codon_start=1
                     /translation="MEQNLPSRITKLIKKSESGDFASSYQLYKVFGSKEYGVEPDEKM
                     SDYFKELSAKQLEGGQLRVADIHLENYKGFESLIMDFSMKKNSTILVGNNGCGKSTIL
                     DAIQKGLTHLSSRLSTRSHNGDGIEKHELRKGQNYASIAINYDYMGIRFPMIIATTEP
                     GYEDRAKSNYSGINELGSIFKTAHSINPNVSFPLIAMYTVERANDVSTRDIENSEEIK
                     EAQIWDKFKAYNKSLTGKADFKLFFRWFKELIEIENSDNADITALRAEIRAKEKDLDN
                     PLLKALLAENKNSETTKKLLEDHQNSLKVLKEKLNSYYSVNSKTLHTVEDAMYSFLPG
                     FSNLKLQRAPLDLIVDKNNVSLSVLQLSQGEKTILALIADIARRLTLLNPNSVNPLDG
                     TGIVLIDEIDLHLHPSWQQNIIPRLEKTFKNIQFIVTTHSPQVCHTIDSQNIWLLKNG
                     QKFKAPKELEEQYLLGYWRTCSKLLKGRQRISTQNSYRNIKI"
BASE COUNT     1194 a    569 c    677 g   1000 t
ORIGIN
        1 gcctctacgc gttttaccga cggcggccag tttggtctgg gtgcggaagt ggcggtaagc
       61 acacaaaaac tccacgcgcg tggcccaatg gggctggaag cactgaccac ttacaagtgg
      121 atcggcattg gtgattacac cattcgtgcg taaataaaac cgggtgatgc aaaagtagcc
      181 atttgattca caaggccatt gacgcatcgc ccggttagtt ttaaccttgt ccaccgtgat
      241 tcacgttcgt gaacatgtcc tttcagggcc gatatagctc agttggtaga gcagcgcatt
      301 cgtaatgcga aggtcgtagg ttcgactcct attatcggca ccatcttttc tcgtaaaatc
      361 attgagttac tattgcagtg ttttgctttg ttcaacacta atgagcaatt ttgcgcgttg
      421 taggtgtggc aagattgtgg caacaataat tgtttaattt agcactacaa agcgaaagcc
      481 tacatgagtt caattgcgtc tcatatttga aattttgtgg gggaaggcgt aagatgacaa
      541 tgctctttag cgttttatgg atttaccacc tgattggtca aatctagttg ggcgttgcgc
      601 aaactctaat ttattgatta catttacagt tgcggaacaa actttttgag ccgcaatcca
      661 gtaggttgcg gatcaaaaag tttgttccgc ggcttcaagt aaagagcctg gacctaggga
      721 tggacaggga acttaaagct ccttgttcat ctcttggtac tagttattga ctggtaaacg
      781 gaacaagctg gtaatgagca tcgacattga aacgacactt caaaaagctt accccgattt
      841 tgacgtactt ttaaaatcaa gaccagcgac acactacaaa gtatacaaaa ttccaaagcg
      901 tacaattggc tatcgtatca tagctcaacc tacgccacga gtaaaagcaa tacaaaggga
      961 tataattgaa atactcaaac agcatactca tatccacgat gcagccacag cgtatgttga
     1021 tgggaagaat atattagata acgccaagat acatcaaagc tcagtttatt tattaaagct
     1081 agatttggtc aattttttta ataaaataac accggaactt ttatttaagg cattagctag
     1141 acaaaaagta gatatttcag ataccaacaa aaatttatta aaacaatttt gtttttggaa
     1201 tagaacaaaa agaaaaaatg gtgcgttagt tttaagtgta ggagcaccaa gctctccttt
     1261 tatatcgaat attgttatga gcagcttcga tgaagagata agttcttttt gcaaggaaaa
     1321 taaaatatct tactcaagat atgctgacga cttaactttt tcaacaaatg aaagagatgt
     1381 tttagggtta gctcatcaaa aagtaaaaac aacattaatt cgtttttttg gcacgcggat
     1441 tattattaat aataacaaga tcgtatattc ttctaaagca cataatcgcc atgtcacagg
     1501 agtgacgtta actaataata ataaattatc actaggaaga gaaaggaaaa gatatattac
     1561 ttctttagta tttaaattta aagaaggcaa actttcgaat gtagatatta atcatcttag
     1621 aggcttgatc ggttttgctt ataatattga gccagctttt attgaaagac ttgagaaaaa
     1681 atatggagaa tcaaccatta agtctatcaa gaaatactct gaaggaggat aaatggaaca
     1741 gaacttaccg agtagaataa ctaaattaat taaaaaatcg gaaagcggcg attttgcttc
     1801 ttcgtatcag ctttataaag tatttggctc gaaagagtac ggagttgagc cagatgaaaa
     1861 aatgtctgat tacttcaaag aattatcagc taaacagtta gaaggtggtc aactgagagt
     1921 tgctgatatt catttggaaa actacaaagg gtttgagtcc ctaataatgg atttttccat
     1981 gaaaaaaaac tctacaattt tagtaggaaa taatggctgt gggaagtcga cgattctcga
     2041 tgcaatccag aaggggttaa cacatctatc ctcgagatta tctactcgct cgcataatgg
     2101 cgatggtatc gaaaaacatg agttaagaaa aggacaaaac tatgcatcga tcgctataaa
     2161 ttacgactat atgggaatac gttttcctat gatcatagct acaactgaac ctggttatga
     2221 ggatagagca aaaagtaact atagcggtat taatgagtta ggaagcattt tcaaaacagc
     2281 ccattcaatc aatccaaatg tttcatttcc tttaattgca atgtatacag ttgaaagggc
     2341 taatgacgtt tctactagag atattgagaa ttcagaagaa attaaagaag ctcaaatctg
     2401 ggataaattc aaagcatata ataagagcct gacaggaaag gctgatttca aattattctt
     2461 cagatggttt aaagaactaa tagaaatcga aaactctgat aacgctgata taacagcatt
     2521 aagagcagag attcgtgcta aagaaaaaga cttagacaat ccattgctaa aagctcttct
     2581 ggcagagaat aaaaattctg agactactaa aaaattgtta gaagatcatc agaactctct
     2641 gaaagtttta aaagagaaat taaatagcta ttattcagtc aatagtaaaa cattacacac
     2701 tgttgaagat gcaatgtatt ctttccttcc tggttttagc aaccttaaac ttcaaagggc
     2761 gcctcttgat ctgatagtgg ataagaataa tgtttcctta agtgttctgc aattatctca
     2821 aggtgaaaaa accattttag cattaattgc agatattgct cgtagattga cattgttaaa
     2881 ccctaatagt gttaaccctt tggacggtac tggaattgta ttaatcgatg aaatagacct
     2941 ccatttacat ccatcatggc agcaaaatat tattcctcgt cttgagaaaa cgtttaagaa
     3001 tattcaattt atagtcacga ctcatagtcc acaagtttgt catactattg atagtcagaa
     3061 tatatggttg ttaaagaatg gccaaaagtt taaagcaccg aaagagttag aggagcaata
     3121 tcttcttggg tactggagaa cttgttcgaa gttgctcaaa ggccgccaga ggataagtac
     3181 acaaaactct tacaggaata taaaaattta gtattttcag aaaaatatgc tagtgaagat
     3241 gcaagaaagc taggtgctac tttatcccaa catttcggac cggatgatga accttagttg
     3301 agttaaagct tgaaattgaa aaaagaattt gggaggatga ttttgaaaag gatcaataaa
     3361 actgctgaag atcaattctt aattaatttt aaagctcaaa atccaaatgg gacttgggat
     3421 gaatttagga atcatgaacg
//