From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Bacillus subtilis orfA, orfB, mreB, mreC, mreD, minC, and minD
Date: 31 Dec 92 01:43:05 GMT
Distribution: bionet
Checksum: 54249 13

LOCUS       BACDIVREG    5641 bp ds-DNA             BCT       18-DEC-1992
DEFINITION  Bacillus subtilis orfA, orfB, mreB, mreC, mreD, minC, and minD
            genes, complete coding regions.
ACCESSION   M96343
KEYWORDS    cell shape determination; division inhibition; homologue;
            minC gene; minD gene; mreB gene; mreC gene; mreD gene; orfA gene;
            orfB gene.
SOURCE      Bacillus subtilis (sub_strain PY79, strain W168) DNA.
  ORGANISM  Bacillus subtilis
            Prokaryotae; Firmicutes; Endospore-forming rods and cocci;
            Bacillaceae.
REFERENCE   1  (bases 1 to 5641)
  AUTHORS   Levin,P.A., Margolis,P.S., Setlow,P., Losick,R. and Sun,D.
  TITLE     Identification of Bacillus subtilis genes for septum placement and
            shape determination
  JOURNAL   J. Bacteriol. 174, 6717-6728 (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     CDS             156..725
                     /gene="orfA"
                     /note="homologous to unidentified E. coli protein"
                     /codon_start=1
                     /translation="MTKPLILASQSPRRKELLDLLQLPYSIIVSEVEEKLNRNFSPEE
                     NVQWLAKQKAKAVADLHPHAIVIGADTMVCLDGECLGKPQDQEEAASMLRRLSGRSHS
                     VITAVSIQAENHSETFYDKTEVAFWSLSEEEIWTYIETKEPMDKAGAYGIQGRGALFV
                     KKIDGDYYSVMGLPISKTMRALRHFDIRA"
     CDS             762..1457
                     /gene="orfB"
                     /note="homologous to E. coli radC gene product and to
                     unidentified protein from Staphylococcus aureus"
                     /codon_start=1
                     /translation="MVIHDLPLKLKDFPMKEKPRERLLKVGAENLANHELLAILLRTG
                     TKHESVLDLSNRLLRSFDGLRLLKEASVEELSSIPGIGMVKAIQILAAVELGSRIHKL
                     ANEEHFVIRSPEDGANLVMEDMRFLTQEHFVCLYLNTKNQVIHKRTVFIGSLNSSIVH
                     PREVFKEAFKRSAASFICVHNHPSGDPTPSREDIEVTRRLFECGNLIGIELLDHLVIG
                     DKKFVSLKEKGYL"
     CDS             1549..2562
                     /function="required for cell shape determination"
                     /gene="mreB"
                     /product="MreB protein"
                     /codon_start=1
                     /translation="MFGIGARDLGIDLGTANTLVFVKGKGIVVREPSVVALQTDTKSI
                     VAVGNDAKNMIGRTPGNVVALRPMKDGVIADYETTATMMKYYINQAIKNKGMFTRKPY
                     VMVCVPSGITAVEERAVIDATRQAGARDAYPIEEPFAAAIGANLPVWEPTGSMVVDIG
                     GGTTEVAIISLGGIVTSQSIRVAGDEMDDAIINYIRKTYNLMIGDRTAEAIKMEIGSA
                     EAPEESDNMEIRGRDLLTGLPKTIEITGKEISNALRDTVSTIVEAVKSTLEKTPPELA
                     ADIMDRGIVLTGGGALLRNLDKVISEETKMPVLIAEDPLDCVAIGTGKALEHIHLFKG
                     KTR"
     CDS             2593..3465
                     /function="required for cell shape determination"
                     /gene="mreC"
                     /product="MreC protein"
                     /codon_start=1
                     /translation="MPNKRLMLLLLCIIILVAMIGFSLKGGRNTTWPEKVIGDTTGVF
                     QNIFHTPAEFFAGIFENINDLKNTYKENERLREKLDGQTQYEAKLQELEEENKSLRDE
                     LGHVKSIKDYKPILATVIARSPDNWAKQVTINKGTQQNVAFDMAVTNEKGALIGKIKS
                     SGLNNFTSAVQLLSDPDRNNRVATKISGKKGSKGYGLIEGYDKEKKRLKMTIIERKDK
                     QDVKKGDLIETSGTGGVFPEGLTIGEVTDIESDSYGLTKVAYVKPAADLTDLNNVIVV
                     NRDVPTVDTEEEGS"
     CDS             3462..3980
                     /function="required for cell shape determination"
                     /gene="mreD"
                     /note="location of rodB1 mutation"
                     /product="MreD protein"
                     /codon_start=1
                     /translation="MKRFLLPFVMMLVFSAESIFTDLVHFPFVTDDQVLAPRFLMLVL
                     IFMSAFINQKHAMIYGFIFGFLYDMNYTSLLGVYMFGFAGLCYLASKAFKVLHTNAFV
                     VILIAVLAVCLLEFYVFGIQSLIHKDIMTFNGFVLDRFIPTILLNIAAALILVLPFRL
                     FFMSLKKELRDE"
     CDS             4033..4713
                     /function="division inhibitor"
                     /gene="minC"
                     /note="mutation results in minicell phenotype"
                     /product="MinC protein"
                     /codon_start=1
                     /translation="MKTKKQQYVTIKGTKNGLTLHLDDACSFDELLDGLQNMLSIEQY
                     TDGKGQKISVHVKLGNRFLYKEQEEQLTELIASKKDLFVHSIDSEVITKKEAQQIREE
                     AEIISVSKIVRSGQVLQVKGDLLLIGDVNPGGTVRAGGNIFVLGSLKGIAHAGFNGNN
                     QAVIAASEMLPTQLRINHVLNRSPDHIQKGNEMECAYLDTDGNMVIERLQHLAHLRPD
                     LTRLEGGM"
     CDS             4715..5521
                     /function="division inhibitor"
                     /gene="minD"
                     /note="location of the divIV-B1 mutation; mutation results
                     in minicell phenotype"
                     /product="MinD protein"
                     /codon_start=1
                     /translation="MGEAIVITSGKGGVGKTTTSANLGTALAILGKRVCLVDTDIGLR
                     NLDVVMGLENRIIYDLVDVVEGRCKMHQALVKDKRFDDLLYLMPAAQTSDKTAVAPEQ
                     IKNMVQELKQEFDYVIIDCPAGIEQGYKNAVSGADKAIVVTTPEISAVRDADRIIGLL
                     EQEENIEPPRLVVNRIRNHLMKNGDTMDIDEIVQHLSIDLLGIVADDDEVIKASNHGE
                     PIAMDPKNRASIAYRNIARRILGESVPLQVLEEQNKGMMAKIKSFFGVRS"
BASE COUNT     1729 a   1067 c   1343 g   1502 t
ORIGIN      
        1 taagagatag tgccctgagc cttcgtcctt tagtgacagg ctttttttat tttgtcatga
       61 aaaagttatt tttctgaaat tgtgacaagc ctcgtgattt ggtaggataa acgagtatct
      121 ttttttctta agcaaaccct gaaaggatga cgaacatgac aaaaccgcta atacttgcat
      181 cacaatctcc gcgcaggaaa gaactcctcg atcttctcca gctgccctac tccattattg
      241 tcagtgaagt agaagaaaaa ttaaatcgaa acttttcacc agaagaaaac gtccaatggt
      301 tggcaaaaca aaaagctaag gctgtagctg atctccaccc gcatgcaatc gttatcggtg
      361 ctgatacaat ggtatgcctt gacggcgaat gtcttggcaa accgcaggat caagaagaag
      421 ctgcttctat gctgcggagg ctttcaggcc gaagtcactc agtaataacc gcggtcagca
      481 ttcaagctga aaatcacagc gagacttttt atgataagac agaagttgcg ttttggtccc
      541 tcagtgaaga agagatttgg acatatattg aaacaaaaga gccgatggat aaagcaggtg
      601 catacggcat ccaaggaaga ggcgcgcttt ttgtaaagaa aatagacgga gattattatt
      661 cagtcatggg ccttcctata tcaaaaacaa tgagagctct tcgccatttt gatataaggg
      721 catgacggtt ctataaacgg gaagggaaag aaaaggagtg cttggtcata cacgatctgc
      781 cattaaaact caaagatttc ccaatgaaag aaaagccaag ggaacggctc ctgaaagtcg
      841 gagccgagaa cttagcgaat catgaacttt tggctatatt attgcggaca gggactaaac
      901 acgaatctgt tttggacctg tcaaaccggc tgctgcgctc atttgacggg ctgcgcctgc
      961 tcaaggaagc atcggttgaa gagctgtcaa gcatcccggg aatcggtatg gtaaaagcga
     1021 ttcaaatact ggcagcagtt gagcttggaa gccggattca taaattagcc aacgaagaac
     1081 atttcgttat tcgctccccg gaagacggcg ctaatcttgt catggaggat atgcgctttt
     1141 taacccagga gcattttgtc tgtttatact taaatacaaa aaatcaagtc atccataaac
     1201 gcaccgtatt tatcggaagc ctgaattcat ctattgtcca cccgcgagag gtgtttaaag
     1261 aagcgtttaa acgatctgcc gcttccttta tctgtgttca taatcatcct tctggagatc
     1321 cgacgccgag cagggaagat attgaagtga caagacggct gtttgaatgc ggaaacctga
     1381 ttggcatcga gctgcttgac catttggtga tcggggataa aaaatttgtg agtttaaagg
     1441 aaaaaggata tttgtaacac tttttttttc gtcgaattaa gctataatag agtttatgag
     1501 tttttccctt tagggtattt ttgctttaag aaaggaagat acatacatat gtttggaatt
     1561 ggtgctagag accttggtat agatcttgga actgcgaata cgcttgtttt tgtaaaagga
     1621 aaaggaattg ttgtgagaga gccgtcagtt gtcgctttgc agacggatac gaaatcgatt
     1681 gtcgctgtcg gaaatgatgc gaaaaatatg attggacgga caccgggcaa cgtggtggct
     1741 cttcgcccga tgaaagacgg cgttatcgct gattatgaaa caacggcgac gatgatgaaa
     1801 tattacatca atcaggccat aaaaaataaa ggcatgttta ccagaaaacc atatgtaatg
     1861 gtatgtgtcc catcaggcat tacagctgtt gaagaacgcg ctgttatcga tgcgacaaga
     1921 caggcgggag cgcgtgacgc gtatccgatt gaagagcctt ttgccgcagc aatcggagcc
     1981 aatctgccag tttgggaacc gactggaagc atggttgttg atatcggggg cggtacgaca
     2041 gaagttgcga ttatttccct cggaggcatc gtaacgtctc agtcaatccg tgtagccggt
     2101 gatgagatgg atgacgcgat tatcaactac atcagaaaaa cgtacaatct gatgatcggt
     2161 gaccgtacgg ctgaagcgat taaaatggaa atcggatctg cagaagctcc tgaagaatcc
     2221 gacaacatgg aaatccgcgg ccgcgatttg ctcacaggtt tgccgaaaac aattgaaatt
     2281 acaggaaaag agatttctaa cgctctacgc gacactgtat ctacaattgt cgaagcagtg
     2341 aagagcacac tcgaaaaaac accgcctgag cttgcagcag atatcatgga cagaggtata
     2401 gtgttaaccg gcggcggagc gcttttgcgc aatttggaca aagtcatcag cgaagaaaca
     2461 aaaatgccgg tccttatcgc cgaagatccg cttgattgtg tagcgatcgg aacagggaaa
     2521 gcactggagc acatccatct tttcaaaggg aaaactagat aatcgggagt tcaatagaag
     2581 aggtgtaaca cgatgccgaa taagcggtta atgctattac ttctgtgtat tatcatattg
     2641 gtggctatga ttggattttc gctgaagggc ggccgcaata ccacctggcc tgagaaagtg
     2701 atcggcgata cgacgggagt atttcaaaat atttttcata cgcctgccga attttttgca
     2761 ggaatatttg agaacatcaa tgatttaaaa aacacataca aagaaaacga gcgtctaaga
     2821 gaaaaacttg acggacagac acaatatgaa gccaagcttc aagagcttga agaagaaaac
     2881 aaatccttgc gtgacgagct tggccatgtc aaatcgatta aagattacaa gccgatttta
     2941 gcaacggtca tcgccagaag ccctgataat tgggcgaaac aggtcaccat taacaagggg
     3001 actcagcaaa acgtagcgtt tgatatggcc gttacaaacg aaaaaggcgc attaatcggc
     3061 aagatcaaaa gctccggact taacaatttt acgtctgctg ttcagctttt aagcgatcct
     3121 gaccgcaata acagagtcgc gacaaaaatt tccggaaaaa aaggcagcaa aggctacggc
     3181 ttgatcgaag gatatgacaa agagaaaaaa cgtcttaaga tgacaattat tgagcgtaag
     3241 gataaacaag acgtgaaaaa aggcgatctt attgaaacat cagggacagg cggtgttttc
     3301 ccagaagggc tgacaatcgg tgaagtgact gatatcgagt cagattccta tggattaacg
     3361 aaggttgctt atgtaaaacc tgcggctgac cttacagatt taaataatgt gatcgttgtt
     3421 aaccgtgacg tgccgactgt cgatacagag gaggaaggat cgtgaaacgt ttccttctcc
     3481 ctttcgttat gatgcttgtt ttttctgcgg aaagcatttt tacagatttg gtgcattttc
     3541 ctttcgttac agatgaccaa gtgctcgccc cgcgtttttt gatgcttgta ttgatattca
     3601 tgtcggcttt tatcaaccaa aaacacgcga tgatttacgg atttattttt ggctttctat
     3661 atgacatgaa ctatacaagt ctattaggcg tttacatgtt tggttttgca gggctatgct
     3721 atttggcttc aaaagcgttt aaagtgttgc atacaaacgc atttgtagtg atattgatag
     3781 cagttctggc tgtctgtctg ctcgaatttt acgtattcgg cattcagtct ttgattcata
     3841 aagacattat gacgtttaac ggatttgtgc ttgaccggtt tataccgaca attttattaa
     3901 atattgcagc agctcttatt cttgttctgc catttagatt gttttttatg agtctaaaga
     3961 aagaattgag agatgagtaa aaaggatttt atcttttttt gacgaaatga gtatgttgtt
     4021 gaggtgaata ttgtgaagac caaaaagcag caatatgtaa caataaaagg aacaaagaat
     4081 ggactaacat tgcatctgga tgatgcgtgt tcttttgatg agcttctcga tggtcttcag
     4141 aatatgctgt caattgaaca atataccgat ggaaaaggcc agaaaatcag cgttcatgtt
     4201 aagctgggaa atcgcttttt atataaggag caagaggaac agctaaccga attgattgcg
     4261 tcaaagaaag atttgtttgt tcattctatt gacagtgaag tcattactaa aaaagaagca
     4321 cagcagataa gagaggaagc cgaaattatt tctgtttcaa aaattgtccg ttcaggccaa
     4381 gtgctgcagg taaaaggcga cttgctcctg atcggtgacg tgaatcccgg cggaacagtc
     4441 agggccggag ggaacatttt tgttctgggc tcactgaaag gaattgcgca tgctggattc
     4501 aatggaaata atcaagcggt catcgccgcc tctgaaatgc ttccgacaca attaagaatc
     4561 aatcatgtgt taaatcgctc cccagaccac attcaaaaag ggaacgaaat ggaatgtgct
     4621 tatttagata cagacggaaa tatggtcatt gaacgccttc aacatttggc tcatttaaga
     4681 cctgatctaa caaggcttga gggaggaatg tgaattgggt gaggctatcg taataacttc
     4741 gggaaaaggc ggagtaggta agacaacaac atctgcgaac ctcggtaccg ccttagccat
     4801 tttagggaag cgcgtatgct tagtagatac tgatatagga ctgcgcaacc ttgatgttgt
     4861 aatgggtctt gaaaatagaa ttatttacga tctggtagac gttgtagagg gcagatgcaa
     4921 aatgcatcag gcgctcgtaa aagacaaacg tttcgatgat ctgctctatt taatgcccgc
     4981 tgctcaaacg agcgataaga cagctgttgc tcctgaacaa attaaaaaca tggtccaaga
     5041 gctcaaacag gaatttgact atgtcatcat agactgtcct gccggaatcg agcaagggta
     5101 caaaaatgcc gtttccggag ctgataaagc gattgtggtc actacgcctg aaatctcagc
     5161 tgttcgtgat gctgaccgta ttataggact gctggagcaa gaggaaaata ttgaaccgcc
     5221 gcggctcgtt gttaacagaa tcagaaatca cctgatgaaa aacggtgaca cgatggatat
     5281 cgacgaaatc gtacagcatc tgtcgatcga tttgctcgga atcgtggctg atgatgatga
     5341 agtcattaaa gcttccaatc atggcgaacc gattgcgatg gaccctaaaa accgcgcttc
     5401 cattgcatat cgcaatattg cccgccgcat cttaggtgaa tctgttcctt tacaggtgct
     5461 tgaagagcaa aacaaaggaa tgatggctaa gattaagtca tttttcggag taagatctta
     5521 atgtgataga atcaaagaga agaatctgac aaagcatatg ctgtgtcagg tttttttttg
     5581 tttttgcctg ctttgttctt gactaaaggg aatatttgcc atggacaaga catatgatgt
     5641 a
//