From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Z.mays ZSF4C2 gene for 22 kD zein
Date: 29 Dec 92 21:27:56 GMT
Distribution: bionet
Checksum: 25135 6

LOCUS       MZEC2ZEIN    3187 bp ds-DNA             PLN       14-DEC-1992
DEFINITION  Z.mays ZSF4C2 gene for 22 kD zein
ACCESSION   X55723
KEYWORDS    storage protein; zein protein.
SOURCE      Zea mays DNA.
  ORGANISM  Zea mays
            Eukaryota; Plantae; Embryobionta; Magnoliophyta; Liliopsida;
            Commelinidae; Cyperales; Poaceae.
REFERENCE   1  (bases 1 to 3187)
  AUTHORS   Liu,C. and Rubenstein,I.
  TITLE     Molecular characterisation of two types of 22 kd alpha-zein genes
            in a gene cluster in maize
  JOURNAL   Mol. Gen. Genet. 234, 244-253 (1992)
  STANDARD  full automatic
COMMENT     *source: strain=W22; *source: stage=after pollination; *source:
            tissue=leaf; *source: clone_library=W22 cosmid; *source: clone=1A;
            *source: is_germline=Y;
               From EMBL    entry ZMC2ZEIN;  dated 03-SEP-1992.
FEATURES             Location/Qualifiers
     CDS             join(1100..1636,1640..1756,1760..1900)
                     /product="22 kD zein"
                     /codon_start=1
                     /translation="MATKILALLALLALFVSATNAFIISQCSLAPSAIIPQFLPPVTS
                     MGFEHPAVHAYRLQQALAASVLQQPIAQLQQQSLAHLTIQTIATQQQQQFLPALSHLA
                     MVNPAAYLQQQLLASNPLALANVVAHQQQQQLQQFLPALSQLAMVNPAAYLQQQQLLS
                     STPLVLANAPTYLQQQLLQNLPALTQLAMANPAAYLQQLLPFNQLIVSNSVAYLQQRQ
                     LLNPLAVATPLVAAFLQQQQLLPYNQFSLMNPVLSRQQPIVGGAIF"
BASE COUNT     1020 a    664 c    524 g    979 t
ORIGIN      
        1 ggatccgatc cagcacagat ccactataat gcatagtggg tgatgcatgc ctaatggtgg
       61 ttgtttagtg ttagtttggg catgttggaa ctaagcaatt gtcgatgcca tacactagaa
      121 gtgaagaatg ttgttagcgt taggagtaga ggtgtcaatg gggaccgata cccgttaagc
      181 catggagaat ttctctatta gatttagggt atgggaagat tttaatttcc atttgctgaa
      241 gttaggtata catctatttg aatcaacagt tacagttcaa cagaaactcc ttccgctata
      301 gtttgttgca tgtcctacct ttttcctaaa ctattttccc taaattgaca tactctctag
      361 aaaatctatc caaattttga agatcaaatc tgttccattg aactattcat gacatatatc
      421 ttggtattgc tttttgtcat cagaatatat tttgaactca gtaaaagtta tacacgattt
      481 ttttagacaa tactaaagca agttgtaatc aagaacactt gaggtgtgcg ccaattgcca
      541 cattagatat aatcattata attttggtca tcctatatgt gtgcattcat ctatgcattc
      601 taggatttca attagtctca atcttgtagt atttgttcct tcattgtcaa atcacttctc
      661 atctaactac tatgcttttt taaccatcag aacgtaactc caacagtatc catttataaa
      721 ggttttgata gcaaacttta catattcata tcatgttaag gttgtcacat gtgtaaaggt
      781 gaagagatca tgcatgtcat tccacgtaga tgaaaagaat tcctatataa aatgacacct
      841 tttcttgtag gtagtggaaa gtatctttcc agcaaagacc atataatcca ataaagttga
      901 taactaaatg tcgaaataga gtagatgcca tatcatttat accttatctg ttgtttggaa
      961 aaaaagacta aatccaaaat atatatgaca tctcacttgt ataaatagct cccaaatcag
     1021 cagttaacca tcacccataa tattttgaac atttagaaac acacacctag cgaagcgcac
     1081 tagcaacgac ctaacaacaa tggctaccaa gatattagcc cttcttgcgc ttcttgccct
     1141 ttttgtgagc gcaacaaatg cgttcattat ttcacaatgc tcacttgctc ctagtgccat
     1201 tattccacaa ttcctcccac cagttacttc aatgggcttc gaacacccag ctgtgcatgc
     1261 ctacaggcta caacaagcgc ttgcggcgag cgtcttacaa caaccaattg cccaattaca
     1321 gcaacaatcc ttggcacatc taacaataca aaccatcgca acgcaacagc aacaacagtt
     1381 cctaccagca ctgagccacc tagccatggt gaaccctgcc gcctacttgc aacagcaact
     1441 gcttgcatcc aacccacttg ctctggcaaa cgtagttgca caccagcaac aacaacagct
     1501 gcaacagttt ctgccagcgc tcagtcaact agccatggtg aaccctgccg cctacctaca
     1561 acagcaacaa ctgctttcat ctaccccgct cgttctggcc aatgcaccta catacctgca
     1621 acaacaattg ttgcaataga atctaccagc tctgactcag ctagctatgg caaaccctgc
     1681 tgcctacttg caacagctgc ttccattcaa ccaactgatt gtgtcgaact ctgttgcgta
     1741 cctacaacaa cgacaatagt tacttaatcc actagcagtg gctaccccat tggtcgctgc
     1801 cttcctacag cagcaacaat tgctgccata caaccagttc tctttgatga accctgtctt
     1861 gtcgaggcag caacccatcg ttggaggtgc catcttttag attacatatg agatgtactt
     1921 gataatggcg ccctcatacc ggcatgtgtt tcctagaaat aatcaatata ttcattgaga
     1981 tttatctcga tatatttctg aactatgttc attatataaa taattgaaaa catcaaatca
     2041 taattataaa ctcatgcttg gtcaatacat agacaatgcg atattacttc aacatcccaa
     2101 tgatgtccta gcccaacctc ttgaatgtta ctgttttaat gcgtgagggt atatttataa
     2161 gatacacgtc actatttgcg ctttttttag tacatacata ttttgtatgt tgatatgata
     2221 tagttatgga cacatgcttt gaccatgaat attcaaatca gttgtacttg cacgaagcaa
     2281 aacataatat aatttaggag tacacttata actgtgtcca aacatgctca cacaaattca
     2341 taccacatta tattttttgg taaatattcc acacatgtat tttttacaag aacccaaatt
     2401 ttgcagacaa atgcagcatt atagacatgt agaattattt gaagcccgtg aacgtaacaa
     2461 caccaaggtc attaaatcaa ctggacccta tgagtaacaa tttcgatatt gcaaacacca
     2521 aattatagaa cttattggct gaaaaattat gagcaatgtg aagttttaat tattatgcca
     2581 taaatatatc aaattatttt tttgaggaac gtaaaaattg catggaatgg gctgcataac
     2641 gtgctagctc acttctatgc taggtagcat taccaaagat tggaatgttg ctgatgaaca
     2701 ccaaacccac tcaaataata tttatatttg ggttgtttag ttgtaaaagt gaagacccaa
     2761 gataaaagta ccaattgtcc aatgacattc gattattttg ttcggggagc acttggtgcg
     2821 tcatttggac tcgtatctta gtccaataga ttgcattttc cttcaatgtg tagaatccga
     2881 caaagtgcat gttctaaaat tgtaaatcta actaaattag aaagcttgtt actaatttga
     2941 tggttaatta ggtgtagctc ataaaactag aaccaacata gtttgcactc acatgttata
     3001 gaaaatagga gtatccaagc acaatttgtg tgagcatcca tgacacaaat tatatataga
     3061 ctgatataat taattccttc aaaaataaaa aaaggaaaaa ataaagtgtt ttttgataat
     3121 gcaggatatc tcctttagct aatgaggagt actaaaaaga tgttgcactt ttgggtggcc
     3181 agaattc
//