From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Saccharomyces cerevisiae nuclear pre-mRNA splicing factor (PRP21)
Date: 29 Dec 92 20:59:22 GMT
Distribution: bionet
Checksum: 50097 5

LOCUS       YSCPRP21A    2180 bp ds-DNA             PLN       11-DEC-1992
DEFINITION  Saccharomyces cerevisiae nuclear pre-mRNA splicing factor (PRP21)
            gene, complete cds.
ACCESSION   L07744
KEYWORDS    PRP21 gene; nuclear pre-mRNA splicing factor.
SOURCE      Saccharomyces cerevisiae (strain SS330) DNA.
  ORGANISM  Saccharomyces cerevisiae
            Eukaryota; Fungi; Eumycota; Hemiascomycetes; Endomycetales;
            Saccharomycetaceae.
REFERENCE   1  (bases 1 to 2180)
  AUTHORS   Arenas,J.E. and Abelson,J.N.
  TITLE     The Saccharomyces cerevisiae PRP21 gene product is required for
            pre-spliceosome assembly
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     CDS             173..1015
                     /function="required for pre-spliceosome assembly"
                     /gene="PRP21"
                     /product="nuclear pre-mRNA splicing factor"
                     /codon_start=1
                     /translation="MEPEDTQLKEDIKTTVNYIKQHGVEFENKLLEDERFSFIKKDDP
                     LHEYYTKLMNEPTDTVSGEDNDRKSEREIARPPDFLFSQYDTGISRRDMEVIKLTARY
                     YAKDKSIVEQMISKDGEARLNFMNSSHPLHKTFTDFVAQYKRVYSFTGQEIKKSKRTI
                     LDNCFERTQYWEFEKDKDREHDKLVELCKIQFAAIPWDKFTQVAKFSIPEDTEIFEGS
                     LDLEQMRLRRVQTGIKLFDSIKPTNEEEKIVSDQGKQKGGDSKGKKRKIRAVGETRLK
                     KSKK"
     CDS             1211..>2180
                     /note="ORF"
                     /codon_start=1
                     /translation="MIDINVNNIFFRSYSVDPNSGHAIYVFDSTYLPASDEIGDKQVY
                     DLLINALMDRLVMKLPQAPYSLVIFSSGFSQRKISWVYGIKMFAKLPKETKFYLQKIF
                     IVHESFFVRSVYQVISNAMNFNFLDSKDSQHDFPSLVHVLDLTSLSELIDITRLRISL
                     NVYLYDYQIREHINVPEEYYNRLTPLAIRQYRQLVFDKIFKKLQNDALLCELIFQKPG
                     NYKKVNIFLDIIKRNNYIDLSQWDIYSLASVWLNYFIKNKAKPLIPIELIPLPIVDDL
                     KFTSETFRKIIKFNQYQDLFMVIIPFFNRIIAHGESTKHDSRTLSKA"
BASE COUNT      761 a    355 c    425 g    639 t
ORIGIN      
        1 aagcttttgt tttcttttgg tttttctatt gtttgtctgt tgaccatttt ttgataaaat
       61 aagaaccttc cttcaacgaa atatttcggc aaaagttagc cttgaaacac ttaaatacag
      121 gaagaaataa ggtaaagcgt aaaactaaca gcaataattt gcagttgtat aaatggaacc
      181 agaagatacc cagctgaaag aggacattaa aaccacagta aattacataa agcagcatgg
      241 tgttgagttt gagaacaaac tgctagaaga tgagagattc tcattcatca agaaagatga
      301 cccactccat gaatactaca ccaaactcat gaatgagcca acagacactg tcagcggtga
      361 agataatgac agaaagagtg agcgagagat agcaaggccg ccggattttc tgttctctca
      421 atatgatact ggaatatccc gaagagacat ggaagtaatc aaattgacag cacgctacta
      481 tgcaaaggat aaaagtattg ttgaacagat gatttcaaag gatggtgaag ccagattaaa
      541 ctttatgaac agctcacatc cgctacataa gacattcact gattttgtcg cccagtacaa
      601 gcgagtatat tcttttactg gtcaagaaat caagaaaagt aagagaacca ttttagataa
      661 ttgttttgag agaacacagt attgggaatt tgagaaagat aaagaccggg aacatgataa
      721 gctcgtagag ttgtgcaaaa tacaatttgc agccattcca tgggataaat ttactcaagt
      781 ggcaaaattt tctatccctg aagacacaga aatcttcgaa ggctctctag atttagaaca
      841 gatgaggctg cgaagagtac aaactggtat taagttattc gatagtatca agccgacaaa
      901 tgaagaagaa aagatagtat ccgaccaagg taagcaaaaa ggaggagata gtaagggcaa
      961 aaagcgtaaa attagagctg tgggagagac aaggttaaag aaaagtaaaa aataagcctc
     1021 atgaaaatca aagttagcta gaagtatgta aaggttgatg atgcaccgta attgagcaca
     1081 tgtatcacta ttaacggcgt acgtattttg gcgttttttc atgttgatgt tattttgtat
     1141 atagaaagtg aaattctcga ggatatggcc ctactggata atactattgg tagcgtgaaa
     1201 gaaggccagg atgatagata tcaacgttaa taacatattt tttagatctt attcagttga
     1261 tcctaactct ggtcacgcaa tttatgtctt tgattcgact tatttgccag catctgatga
     1321 aattggcgat aagcaagttt atgacttgct tattaatgcc ctcatggatc gattggtaat
     1381 gaaattgcca caggcaccat attcactggt gattttttca tctgggtttt cccagagaaa
     1441 aataagctgg gtttacggta ttaaaatgtt tgcaaagtta cccaaagaga ccaagttcta
     1501 tttacaaaag atttttattg ttcatgagtc attcttcgtt aggtcggtct atcaagtaat
     1561 ttcaaacgct atgaacttta acttcttgga ctcaaaagat agtcagcatg attttccaag
     1621 tttagttcat gtcttggacc tgacttcttt atccgagcta atagatatta cgcgacttag
     1681 aatatcgttg aacgtgtact tatatgatta ccagatacgc gaacatataa atgtcccgga
     1741 ggaatattat aacagattga cccctctggc aataagacag tataggcagt tagtattcga
     1801 taaaattttc aagaaattac agaatgacgc tctgttgtgt gaattgatat tccagaagcc
     1861 gggaaactat aaaaaggtaa acattttttt ggatattatc aaaagaaaca actacattga
     1921 tttatctcaa tgggatattt attctttggc atccgtgtgg ttgaattact tcattaagaa
     1981 taaagccaaa cctctaatcc caattgaatt aattccgtta ccaattgttg atgatctaaa
     2041 gttcacaagt gaaacattcc gcaaaattat taaattcaac caataccagg acctctttat
     2101 ggtcataatt ccttttttta ataggatcat agcgcatggt gaatcgacta aacatgattc
     2161 aaggacccta agtaaagctt
//