From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: D.pseudoobscura Adh gene for alcohol dehydrogenase
Date: 28 Dec 92 21:27:49 GMT
Distribution: bionet
Checksum: 37290 8

LOCUS       DROADHGN65   3475 bp ds-DNA             INV       01-DEC-1992
DEFINITION  D.pseudoobscura Adh gene for alcohol dehydrogenase
ACCESSION   X64474
KEYWORDS    ADH gene; alcohol dehydrogenase.
SOURCE      Drosophila pseudoobscura DNA.
  ORGANISM  Drosophila pseudoobscura
            Eukaryota; Animalia; Metazoa; Arthropoda; Uniramia; Insecta;
            Pterygota; Neoptera; Holometabola; Diptera; Brachycera;
            Cyclorrhapha; Schizophora; Drosophiloidea; Drosophilidae.
REFERENCE   1  (bases 1 to 3475)
  AUTHORS   Schaeffer,S.W. and Miller,E.L.
  TITLE     Estimates of gene flow in Drosophila pseudoobscura determined from
            nucleotide sequence analysis of the alcohol dehydrogenase region
  JOURNAL   Genetics 132, 471-480 (1992)
  STANDARD  full automatic
COMMENT     *source: strain=PS220; *source: isolate=Kiabab National Forest,
            Arizona; *source: chromosome=IV section 88;
               From EMBL    entry DPADHGN65;  dated 16-OCT-1992.
FEATURES             Location/Qualifiers
     mRNA            join(40..119,915..1020,1084..1488,1551..1966)
                     /note="adult mRNA (Adh)"
     mRNA            join(882..1020,1084..1488,1551..1966)
                     /note="larval mRNA (Adh)"
     promoter        8..14
                     /note="adult promoter (Adh)"
     prim_transcript 40..1966
                     /note="adult primary transcript (Adh)"
     exon            40..119
                     /note="adult exon 1a"
     intron          120..914
                     /note="adult intron"
     prim_transcript 882..1966
                     /note="larval primary transcript (Adh)"
     exon            882..1020
                     /note="larval exon 1"
     exon            915..1020
                     /note="adult exon 1b"
     intron          1021..1083
                     /number=1
     exon            1084..1488
                     /number=2
     intron          1489..1550
                     /number=2
     exon            1551..1966
                     /number=3
     polyA_signal    1943..1948
     polyA_site      1966
     CDS             join(928..1020,1084..1488,1551..1817)
                     /EC_number="1.1.1.1"
                     /gene="Adh"
                     /product="alcoholdehydrogenase"
                     /codon_start=1
                     /translation="MSLTNKNVVFVAGLGGIGLDTSRELVKRNLKNLVILDRIDNPAA
                     IAELKAINPKVTITFYPYDVTVPVAETTKLLKTIFAQVKTIDVLINGAGILDDHQIER
                     TIAVNYTGLVNTTTAILDFWDKRKGGPGGIICNIGSVTGFNAIYQVPVYSGSKAAVVN
                     FTSSLAKLAPITGVTAYTVNPGITKTTLVHKFNSWLDVEPRVAEKLLEHPTQTSQQCA
                     ENFVKAIELNKNGAIWKLDLGTLEPITWTQHWDSGI"
BASE COUNT      980 a    790 c    792 g    913 t
ORIGIN      
        1 cgcagtgtat ttaaggcact gcacatgtcg aggaccacga ttattgtcta cagtgcagtc
       61 agtagttgtc aggtgcagca ggcggaccag catttcttta aatctactca atcgatcaag
      121 taagtaattg taatccaagt acagttggcc catcccactc ggttctgccg taactcaaga
      181 aatttcttga tagcgcagtt ccagttgtaa aacgtcgttt aaaaaaacat ctctttggag
      241 cttgggttgg aaaacgttcg agttgggcgt agaaaagttc aaatataaaa tatcaaaaaa
      301 gagaaaatat cttcccaaag cagctgtcca ttttttgtta aagtgataga tcaaaaattc
      361 gtatgacgta tttgccaagt gctctttatg caaataattt cctcaattct aattctacaa
      421 gaagcacttt tggctcttgt ccagaaaaaa caatttgttt acacttttcg gttgataaca
      481 taagcggacc gacggctaag taacataaca gtcgcctgtt aagtaatgcc ctcactctct
      541 gttagatggc cgcgcgtgtc gactccgata gaccagcaga cacgcagtag ttttcctcaa
      601 gaacctgaac tctaaacata gacataattt acgatacctt gagactcaaa taaaaataca
      661 agtatgagtg gtattctatt cctgattaga ctcgcacaca cacacgtacg agtattaaca
      721 taaatttgcc ggcaaataac caaaacaaaa cgaacgcaaa cgatccgaac acgtaatgcg
      781 agagataaga aacagaaaag ctcgacgtga aagaagcttc tggcggaagc tcttgccgaa
      841 ctccctataa atacgagaca gaaaaccaga ataaatataa taacaaactg ttgaattgtc
      901 ccatcgattc ccagatcaaa agacagaatg tctctcacca acaagaacgt cgttttcgtg
      961 gccggtctgg gcggcattgg cctggacacc agccgggagt tggtcaagcg taatctgaag
     1021 gtaagagaga acgaattcca tggaatctat ggaatcctaa atttaaaaat tcatttattt
     1081 tagaacctgg tcatcctgga tcgcattgac aatccggctg ccattgccga actgaaggca
     1141 atcaatccca aggtgaccat caccttctat ccctacgatg tgactgtgcc cgtcgctgag
     1201 accaccaagc tcctgaagac catctttgcc caggtgaaga caatcgatgt cctgatcaac
     1261 ggtgctggca tcctggacga tcatcagatc gagcgcacca ttgccgttaa ctacacgggc
     1321 ctggtcaaca ccaccacagc cattctggac ttctgggaca agcgcaaggg cggcccaggc
     1381 ggcatcattt gcaacattgg ctccgtcacc ggtttcaatg ccatctacca ggtgcccgtt
     1441 tactccggct ccaaggcggc ggtggttaac tttacctcct ccctggcggt aagaaatcct
     1501 acaactatcc caaaatcccc aaaactaaat atttctaaat attcccatag aaactggctc
     1561 ccattactgg tgtcactgct tacactgtca atcctggcat cactaagacc actctggtcc
     1621 acaaattcaa ctcgtggctg gatgtggagc cccgtgtggc agagaagctg ctcgagcatc
     1681 ccacccagac ctctcagcag tgcgccgaga actttgtgaa ggccattgag ctgaacaaga
     1741 acggtgccat ctggaagttg gatctgggca ccttggagcc catcacatgg acccagcact
     1801 gggattcggg catctaaacc atcccagaga ctctatggga catggcgttt tagcttttag
     1861 tttctttttt tccactcaat tgttacgtat atacatacac atatggcaat aaggctgatt
     1921 tgaaccccgt tttgaatatg ataatacaaa attatatttg agaaatttca acaaaatcga
     1981 taagagctat agctctcttt taattaaaaa aaactagata aaaagagcaa tggtcgtcag
     2041 tggtggtcag tggtcgttgt ggcctctatc gatttccaca caaaaacttt actgtttagt
     2101 aatagaaaag aacttgagaa aggcagccaa aagaatgtac gatctgacgg gtaagcatgt
     2161 ctgctatgtg gctgactgcg gtggcattgc actggagacc agcaaggttc tcatgaccaa
     2221 gaatatagcg gtgagtggta gagtggtgta gagagtggag agtgttccga agtggttctg
     2281 gttcttaaac aagtctctag tctctagtct ctagtctctt ggctgctgct ggtagctgtc
     2341 gaaactaatt aagttaatga ctttttcatg ttttgttttt gtccgttcaa agctacaatt
     2401 aagtttatgt tttgagtttg atctactttt gcttaaggga agtactttcg attacggatt
     2461 gctggcatat aaaaatagat accaaggaat gtgcatcata tcatattgat tctatcttga
     2521 ttctttgcct agaaactggc catccttcag agcgtggaaa accccccggc catcgctcag
     2581 ttacaatcga ttaagcacag cacacagatc ttcttctgga ccttcgatgt gaccatggcc
     2641 cgcgaggaga tgaagaagta cttcgatgag gtcatggtcc agatggacta catcgatgta
     2701 ctgatcaatg gggccaccct gtgcgatgag aggaacattg atgccaccat caatacgaat
     2761 ctgaccggca tgatgaacac cgtggccact gtgctgccct atatggaccg aaagatgggc
     2821 ggttcgggtg gactgattgt gaatgtcacc tccgtcatag gactggatcc atcgccggtc
     2881 ttttgtgcgt acagtgcctc aaagtttggt gtgattgggt tcacccgaag tctagcggtg
     2941 agtgggcgag atcttagctt gaaaacgctc tctaatatct tccattcttt cgcaggatcc
     3001 cctgtattac acccaaaatg gtgtggctgt catggccgtc tgctgtggcc ccaccaaagt
     3061 gtttgtcgat cgggaactga ctgccttcct ggaatacggc cagtcctttg ccgatcgcct
     3121 gcgtcgtgcc ccctgccaat cgacggccgt ctgcggccag aatatagtaa atgccatcga
     3181 gagatcggag aacggacaga tctggattgc cgacaagggc ggcctggagt cggtggccct
     3241 ccactggtat tggcatatgg ccgatcagtt tgtgaactat atgcagagca ctgacgatga
     3301 ggatcaggag ttttttttgg gccagcgatg aggcatgcct tgatccacgg aataaaacat
     3361 caaatgtgtc acaaaaagta ggcaacttgt ttgtgggttt ggaaatagat ttgaaatatt
     3421 ttgaggtcat atgaataatg acataaatga gggtgcctga aagtatgcca agttt
//