From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: B.licheniformis glutamic acid-specific protease gene.
Date: 9 Jan 93 05:45:38 GMT
Distribution: bionet
Checksum: 16154 4

LOCUS       BACGASP      1448 bp ds-DNA             BCT       09-JAN-1993
DEFINITION  B.licheniformis glutamic acid-specific protease gene.
ACCESSION   D10060
KEYWORDS    glutamic acid-specific protease.
SOURCE      Bacillus licheniformis (strain ATCC 14580) DNA.
  ORGANISM  Bacillus licheniformis
            Prokaryotae; Bacteria; Firmicutes; Endospore-forming rods and
            cocci; Bacillaceae.
REFERENCE   1  (bases 1 to 1448)
  AUTHORS   Kakudo,S., Kikuchi,N., Kitadokoro,K., Fujiwara,T., Nakamura,E.,
            Okamoto,H., Shin,M., Tamaki,M., Teraoka,H., Tsuzuki,H. and
            Yoshida,N.
  TITLE     Purification, characterization, cloning and expression of a
            glutamic acid-specific protease from Bacillus licheniformis ATCC
            14580
  JOURNAL   J. Biol. Chem. 267, 23782-23788 (1992)
  STANDARD  full automatic
COMMENT     Submitted (09-Dec-1991) to DDBJ by: Etsuo Nakamura Shionogi
            Research Laboratories Shionogi & Co. Ltd. Fukushima-ku, Osaka 553
            Japan Phone:  06-458-5861 x571 Fax:    06-458-0987 E mail:
            nakamura%shiogw.lab.shionogi.co.jp@kddlabs.co.jp.
FEATURES             Location/Qualifiers
     mat_peptide     605..1270
                     /product="glutamic acid-specific protease mature peptide"
                     /codon_start=1
     CDS             323..1273
                     /gene="BLase"
                     /note="ttg start codon, prepro sequence"
                     /product="glutamic acid-specific protease"
                     /codon_start=1
                     /translation="MVSKKSVKRGLITGLIGISIYSLGMHPAQAAPSPHTPVSSDPSY
                     KAETSVTYDPNIKSDQYGLYSKAFTGTGKVNETKEKAEKKSPAKAPYSIKSVIGSDDR
                     TRVTNTTAYPYRAIVHISSSIGSCTGWMIGPKTVATAGHCIYDTSSGSFAGTATVSPG
                     RNGTSYPYGSVKSTRYFIPSGWRSGNTNYDYGAIELSEPIGNTVGYFGYSYTTSSLVG
                     TTVTISGYPGDKTAGTQWQHSGPIAISETYKLQYAMDTYGGQSGSPVFEQSSSRTNCS
                     GPCSLAVHTNGVYGGSSYNRGTRITKEVFDNLTNWKNSAQ"
BASE COUNT      431 a    360 c    294 g    363 t
ORIGIN      
        1 tcgacggctt cccgtgcgcc tccgggatcg ctgtgataat tgacaaccac attcatcttt
       61 tcttttccaa accgttctgc aaccgccttg cctatacctt ttgaagagcc ggtcacaatt
      121 gctgtttttc cttttaaatc actatacaac ctaaacaccc ctcaatttct tttctccatg
      181 tacattaccc ggtatcaata tatgatcaaa caaaatgtta atacacacct ttagtatgat
      241 cttttttaaa catatggaaa attcagaatt attttgttaa tatctaactt gtacttacaa
      301 caaaataagg aagtgatatg atttggttag taaaaagagt gttaaacgag gtttgatcac
      361 aggtctcatt ggtatttcta tttattcttt aggtatgcac ccggcccaag ccgcgccatc
      421 gcctcatact cctgtttcaa gcgatccttc atacaaagcg gaaacatcgg ttacttatga
      481 cccaaacatt aagagcgatc aatacggctt gtattcaaaa gcgtttacag gcaccggcaa
      541 agtgaatgaa acaaaggaaa aagcggaaaa aaagtcaccc gccaaagctc cttacagcat
      601 taaatcggtg attggttctg atgatcggac aagggtcacc aacacaaccg catatccgta
      661 cagagcgatc gttcatattt caagcagcat cggttcatgc accggatgga tgatcggtcc
      721 gaaaaccgtc gcaacagccg gacactgcat ctatgacaca tcaagcggtt catttgccgg
      781 tacagccact gtttcgccgg gacggaacgg gacaagctat ccttacggct cagttaaatc
      841 gacgcgctac tttattccgt caggatggag aagcggaaac accaattacg attacggcgc
      901 aatcgaacta agcgaaccga tcggcaatac tgtcggatac ttcggatact cgtacactac
      961 ttcatcactt gttgggacaa ctgttaccat cagcggctac ccaggcgata aaacagcagg
     1021 cacacaatgg cagcattcag gaccgattgc catctccgaa acgtataaat tgcagtacgc
     1081 aatggacacg tacggaggac aaagcggttc accggtattc gaacaaagca gctccagaac
     1141 gaactgcagc ggtccgtgct cgcttgccgt acacacaaat ggagtatacg gcggctcctc
     1201 gtacaacaga ggcacccgga ttacaaaaga ggtgttcgac aatttgacca actggaaaaa
     1261 cagcgcacaa taatacacga agacagcccg cttccttttg gaacgggctg tcacatctaa
     1321 cggccgtata cttaatttcc tttaagcctg tactttttgc catctattga tatcgtgaaa
     1381 tttgaaggac cgctgatcgg caaataatag acaagctgaa actccgcttc ctcaccaggt
     1441 ttgaatgg
//