From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: B. laterosporus glutaryl 7-ACA acylase gene, complete cds.
Date: 3 Jan 93 22:11:37 GMT
Distribution: bionet
Checksum: 15115 6

LOCUS       BACG7AA      2466 bp ds-DNA             BCT       23-DEC-1992
DEFINITION  B. laterosporus glutaryl 7-ACA acylase gene, complete cds.
ACCESSION   D10200 D90431
KEYWORDS    cephalosporin acylase; glutaryl 7-ACA acylase.
SOURCE      Bacillus laterosporus (strain J1) genomic DNA, clone pACYJ1-3
  ORGANISM  Bacillus laterosporus
            Prokaryotae; Firmicutes; Endospore-forming rods and cocci;
            Bacillaceae.
REFERENCE   1  (bases 1 to 2466)
  AUTHORS   Aramori,I., Fukagawa,M., Tsumura,M., Iwami,M., Ono,H., Kojo,H.,
            Kohsaka,M., Ueda,Y. and Imanaka,H.
  TITLE     Cloning and nucleotide sequencing of a novel 7bata-
            (4-carboxybutanamido) cephalosporanic acid acylase gene of Bacillus
            laterosporus and its expression in Excherichia coli and Bacillus
            subtilis
  JOURNAL   J. Bacteriol. 173, 7848-7855 (1991)
  STANDARD  full automatic
COMMENT     Submitted (27-Jun-1991) to DDBJ by: Hitoshi Kojo Exploratory
            Research Laboratories Fujisawa Pharmaceutical Co.,Ltd. 5-2-3
            Tokodai Tsukuba Ibaraki 300-26 Japan Phone:  0298-47-8611 Fax:
            0298-47-8610.
FEATURES             Location/Qualifiers
     -35_signal      118..128
     -10_signal      143..148
     RBS             194..201
     sig_peptide     208..288
                     /codon_start=1
     mat_peptide     289..2109
                     /EC_number="3.5.1.11"
                     /product="glutaryl 7-ACA acylase"
                     /evidence="EXPERIMENTAL"
                     /codon_start=1
     terminator      2120..2141
     CDS             208..2112
                     /product="glutaryl 7-ACA acylase precursor"
                     /codon_start=1
                     /translation="MNRKKKFLSMLLTVLLVTSLFSSVAFGQSEQEKAEELYQYELKT
                     DVMVEMRDGVKLATDIYLPVAKTEQEKKDGFPTLVFRTPYNKDTYGKTEGPFFAKRGY
                     AVVVQDTRGRYKSEGEWNFVFDDAKDGYDLIEWAAVQDFSTGKVGTMGLSYMAYTQYV
                     LAESKPPHLVTMIPLEGMSNPAEEVFFTGGAMQLDRYLSWTLGQAVDTARRLDEKNGN
                     TVNQDKIKKALDDYEKWLNHMPRSKVAPLNQMIDWWKEAMDHPEYDEYWKSISPQEQH
                     DTWPVPTYHVGGWYDILLNGTSKNYIGITENGPTERYLPALEKTVNIQDTQKLLIGPW
                     THGYPQTAVGTFNFPKADLSDVHNAGNGADNWRLEQLRWFDYWLKGIDNGIMDEDPVK
                     LYIMKGENDGFWRTEKEWPIARTEYTNYYLHDGKSGTIDSLNDGILSTEKPKSGKKAD
                     SYLYDPKNPTPTVGGNISGTTPNDERGPQDQQGIEKDVLTYTTEVLNEDTEVTGPIKV
                     KLWASTNAKDTDFAVKLTDVYPDGRSIIIQDSIIRGRYHESREKETLLEPGKIYEFTI
                     DLGSTANIFKKGHRIRVDVSSSNYPRFDNNPNTGHKFGNDAAMKTAKNTIYHDSEHPS
                     HIILPIIPNE"
BASE COUNT      817 a    437 c    552 g    660 t
ORIGIN      
        1 acattgacag ttaagcaatt tttattaaat attacatacc aacttccaca tatatcaata
       61 aggtttatac tttattgata tagcaactat aataatccaa ctaaatacct atatcctttt
      121 tcccgcgaat gtcctattta cttatttttc ctatcgatat aatattagtt tgaaaatttt
      181 aaaaataaag aaaatggagg tgtgtgcatg aatagaaaga aaaaatttct ttctatgctg
      241 ctgactgttc ttttagtcac atcattattt agcagtgtgg cttttgggca gtcagagcag
      301 gaaaaggcag aagaacttta tcagtatgaa cttaagactg acgttatggt agaaatgcgc
      361 gatggtgtaa aactggctac agatatttac ctgccggttg ccaaaacgga gcaagaaaag
      421 aaagatggtt ttcctacgct tgtgtttcga actccttaca acaaggatac atatgggaaa
      481 actgaaggtc ctttctttgc aaaaagaggc tatgcagtgg ttgttcagga tacacgtggc
      541 cgctacaagt cagaaggaga atggaacttt gtatttgatg atgccaagga tggctatgat
      601 ttaattgaat gggctgcagt tcaggatttc agtactggga aggttggcac aatgggccta
      661 tcttacatgg cctataccca gtatgtattg gctgaatcaa aaccgcctca tcttgttaca
      721 atgattccgc ttgaagggat gagcaatcct gctgaagaag tcttttttac aggcggagct
      781 atgcagctag accgctattt atcatggact ttgggccagg cggtagatac agcaagacga
      841 cttgacgaaa agaatggaaa tactgttaac caggataaga ttaaaaaagc gttagatgat
      901 tatgagaagt ggcttaatca tatgccaaga tctaaggtgg caccattaaa ccaaatgatt
      961 gattggtgga aagaagcgat ggatcatcct gagtatgacg agtattggaa gagcatctct
     1021 cctcaggaac aacatgatac atggccagta ccaacctatc atgttggggg atggtacgat
     1081 attttactaa acggaacatc taaaaactat attgggatta cagaaaatgg tccgacagaa
     1141 agatatttgc ctgctttaga gaaaaccgta aacattcaag acacgcaaaa attattaatt
     1201 ggaccatgga ctcacggata tccgcaaaca gcggtgggga cgtttaattt tccaaaagct
     1261 gatttgagcg atgtgcacaa tgctggcaat ggggcagata attggcggct tgagcaatta
     1321 cgctggtttg attactggct aaaaggaata gataacggaa ttatggatga agatccggtc
     1381 aagctttata ttatgaaggg tgaaaatgat ggcttctggc gcacggaaaa ggagtggccg
     1441 atagctcgca ccgaatatac aaactactat cttcatgatg gtaaatctgg aacgattgat
     1501 tcattgaatg atggcattct gagcaccgaa aagccaaaat ctggtaaaaa agctgattct
     1561 tatctttatg atccgaaaaa cccaacgccg actgtgggcg gaaatattag cggaacgaca
     1621 ccaaatgatg agcgaggtcc acaagatcag cagggtattg aaaaagatgt gcttacctac
     1681 acaacagagg tgctgaatga ggacacagaa gtaactggcc cgattaaggt gaagctttgg
     1741 gcatcaacta acgctaagga cactgacttt gctgttaaat taacggatgt ctatcctgac
     1801 ggacgttcca tcatcattca agacagcatt atccgcggcc gataccatga atcccgtgaa
     1861 aaagaaacct tattagagcc agggaaaatc tatgaattta cgattgacct aggctcaacg
     1921 gctaatatat ttaaaaaggg acatcgcatc cgtgtagatg tttccagcag taactatcct
     1981 agattcgata ataacccgaa tacaggacat aagtttggca atgatgccgc tatgaagaca
     2041 gcgaaaaata cgatttatca tgattcagag catccgtcac atattatatt gccaattatt
     2101 ccaaatgaat aatttcaagg ggctggctca cgtgccagcc ctgttttttt tcaaaagctt
     2161 ttgcaaaata gggaaaattg ctcaataata gaattgtaca taaaggggga atcagcaatg
     2221 agaggaatta ttcacaacgc agcacgtgaa atgtcaaagg aagatgtgga aacattttta
     2281 caacaagccg aagtggtcca tgtggctaca accggaaaag acggctaccc atatgtcatt
     2341 cctttggtgt atgtctatga aggcggtcct aagttttata ttcatacagg caatctgaga
     2401 gaaagccatt ttgaacagaa tattaaagaa aatcctcgag tgtgtattga ggtagagcaa
     2461 atgggt
//