From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: D.pseudoobscura Adh gene for alcohol dehydrogenase
Date: 28 Dec 92 21:28:10 GMT
Distribution: bionet
Checksum: 32795 8

LOCUS       DROADHGN80   3472 bp ds-DNA             INV       01-DEC-1992
DEFINITION  D.pseudoobscura Adh gene for alcohol dehydrogenase
ACCESSION   X64489
KEYWORDS    ADH gene; alcohol dehydrogenase.
SOURCE      Drosophila pseudoobscura DNA.
  ORGANISM  Drosophila pseudoobscura
            Eukaryota; Animalia; Metazoa; Arthropoda; Uniramia; Insecta;
            Pterygota; Neoptera; Holometabola; Diptera; Brachycera;
            Cyclorrhapha; Schizophora; Drosophiloidea; Drosophilidae.
REFERENCE   1  (bases 1 to 3472)
  AUTHORS   Schaeffer,S.W. and Miller,E.L.
  TITLE     Estimates of gene flow in Drosophila pseudoobscura determined from
            nucleotide sequence analysis of the alcohol dehydrogenase region
  JOURNAL   Genetics 132, 471-480 (1992)
  STANDARD  full automatic
COMMENT     *source: strain=PS297; *source: isolate=Kiabab National Forest,
            Arizona; *source: chromosome=IV section 88;
               From EMBL    entry DPADHGN80;  dated 16-OCT-1992.
FEATURES             Location/Qualifiers
     mRNA            join(40..119,914..1019,1084..1488,1549..1964)
                     /note="adult mRNA (Adh)"
     mRNA            join(881..1019,1084..1488,1549..1964)
                     /note="larval mRNA (Adh)"
     promoter        8..14
                     /note="adult promoter (Adh)"
     prim_transcript 40..1964
                     /note="adult primary transcript (Adh)"
     exon            40..119
                     /note="adult exon 1a"
     intron          120..913
                     /note="adult intron"
     prim_transcript 881..1964
                     /note="larval primary transcript (Adh)"
     exon            881..1019
                     /note="larval exon 1"
     exon            914..1019
                     /note="adult exon 1b"
     intron          1020..1083
                     /number=1
     exon            1084..1488
                     /number=2
     intron          1489..1548
                     /number=2
     exon            1549..1964
                     /number=3
     polyA_signal    1941..1946
     polyA_site      1964
     CDS             join(927..1019,1084..1488,1549..1815)
                     /EC_number="1.1.1.1"
                     /gene="Adh"
                     /product="alcoholdehydrogenase"
                     /codon_start=1
                     /translation="MSLTNKNVVFVAGLGGIGLDTSRELVKRNLKNLVILDRIDNPAA
                     IAELKAINPKVTITFYPYDVTVPVAETTKLLKTIFAQVKTIDVLINGAGILDDHQIER
                     TIAVNYTGLVNTTTAILDFWDKRKGGPGGIICNIGSVTGFNAIYQVPVYSGSKAAVVN
                     FTSSLAKLAPITGVTAYTVNPGITKTTLVHKFNSWLDVEPRVAEKLLEHPTQTSQQCA
                     ENFVKAIELNKNGAIWKLDLGTLEPITWTQHWDSGI"
BASE COUNT      969 a    786 c    798 g    919 t
ORIGIN      
        1 cgtagtgtat ttaaggcact gcacatgtcg aggaccacga ttattgtcta cagtgcagtc
       61 agtagttgtc aggtgcagca ggcggaccag catttcttta aatctactca atcgatcaag
      121 taagtaactg taatccgagt acagtcgacc catcccactc ggttctgcag taactcaaga
      181 aatttcttga tagcgcagtt ccatttgtag aacgtcgttt aaaaaatcat ctcgttggag
      241 cttgggttgg aaaacgttcg agttgggcgt agaaaagttc aaatataaaa tataaaaaag
      301 agaaaatatc ttcacaaagc agctgtccat tttttgttaa agtgatagat caaaaattcg
      361 tatgacgtat ttcccaagtg ctctttatgc aaataatttc ctcaattcta attctacaag
      421 aagcactttt ggcacttgtc cagaaaaaac aatttgttta cacttttcgg ttgataacag
      481 aggcggaccg acggctaagt aacataacag tcgcctgtta agtaatgccc tcactcgctg
      541 ttagatggcc gcgcgtgtcg actccgatag accagcagac acgcagtagt tttcctcaag
      601 aacctgaact ctaaacatag acataattta cgataccttg agactcaaat aaaaatacat
      661 gtatgagtgg tattctattc ctgattagac tcgcacacac acacgtacga gtattaacat
      721 aaatttgccg gcaaataacc aaaacaaaac gaacgcaaac gatccgaaca cgtaatgcga
      781 gagataagaa acagaaaagc tcgacgtgaa agaagcttct ggcggaagct cttgccgaac
      841 tccctataaa tacgagacag aaaaccagaa taaatataat aacaaactgt tgaattgtcc
      901 catcgattcc cagatcaaaa gacagaatgt ctctcaccaa caagaacgtc gttttcgtgg
      961 ccggtctggg cggcattggc ctggacacca gccgggagtt ggtcaagcgt aatctgaagg
     1021 taagagagaa cgaattccat ggaatctatg gaaatcctaa atttaaaaat ttattttttt
     1081 tagaacctgg tcatcctgga tcgcattgac aatccggctg ccattgccga actgaaggca
     1141 atcaatccca aggtgaccat caccttctat ccctacgatg tgactgtgcc cgtcgctgag
     1201 accaccaagc tcctgaagac catctttgcc caggtgaaga ccatcgatgt cctgatcaac
     1261 ggtgctggaa tcctggacga tcatcagatc gagcgcacca ttgccgttaa ctacacgggc
     1321 ctggtcaaca ccaccacagc cattctggac ttctgggaca agcgcaaggg cggcccaggc
     1381 ggcatcattt gcaacattgg ctccgtcacc ggtttcaatg ccatctacca ggtgcccgtt
     1441 tactccggct ccaaggcggc ggtggttaac tttacctcct ccctggcggt aagtaatcca
     1501 acgtttttcc tactccccaa aactaaatat ttctttgaaa tcccatagaa actggctccc
     1561 attactggtg tcactgctta cactgtcaat cctggcatca ctaagaccac tctggtccac
     1621 aaattcaact cgtggctgga tgtggagccc cgtgtggcag agaagctgct cgagcatccc
     1681 acccagacct ctcagcagtg cgccgagaac tttgtgaagg ccattgagct gaacaagaac
     1741 ggtgccatct ggaagttgga tttgggcacc ttggagccca tcacatggac ccagcactgg
     1801 gactcgggca tctaaaccat cccagagact ctatgggaca tggcgtttta gcttttagtt
     1861 tctttttttc cactcaattg ttacgtatat acatacacat atggcaataa ggctgatttg
     1921 aaccccgttt tgaatatgat aatacaaaat tatatttgag aaatttcaac aaaatcgata
     1981 agagctatag ctctctttta attaaaaaaa aactagataa aaagagcaat ggtcagtggt
     2041 ggtcagtggt cgttgtggcc tctatcgatt tccacacaaa aactttactg tttagtaata
     2101 gaaaagaact tgagaaaggc agccaaaaga atgtacgatc tgacgggtaa gcatgtctgc
     2161 tatgtggctg actgcggtgg cattgcactg gagaccagca aggttctcat gaccaagaat
     2221 atagcggtga gtggtagaga ggtggtgtag agagtggaga gtggttctgg ttcttaaaca
     2281 agtctctagt ctctagtctc tagtctcttg gctgctgctg gtagctgtcg aaactaatta
     2341 agttaatgac tttttcatgt tttgtttttg tccgctgact tttgctgttc aaagctacaa
     2401 ttaagtttat gttttgagtt tgatctactt ttgcttaagg gaagtacttt cgattacgga
     2461 ttgctggcat ataaaaatag ataccaagga atgtgcatca tattgattct atcttgattc
     2521 tttgcccaga aactggccat ccttcagagc gtggaaaacc ccccggccat cgcccagtta
     2581 caatcgatta agcacagcac acagatcttc ttctggacct tcgatgtgac catggcccgc
     2641 gaggagatga agaagtactt cgatgaggtc atggtccaga tggactacat cgatgtgctg
     2701 atcaatgggg ccaccctgtg cgatgagcgg aacattgatg ccaccatcaa tacgaatctg
     2761 accggcatga tgaacaccgt ggccactgtg ctgccctata tggaccgtaa gatgggcggt
     2821 tcgggtggac tgattgtgaa tgtcacctcc gtcataggac tggatccatc gccggtcttt
     2881 tgtgcgtaca gtgcctcaaa gtttggtgtg attgggttca cccgaagtct agcggtgagt
     2941 gggcgagatc ttaacttgaa aatgctctct aatgtctttc attctttcgc aggatcccct
     3001 gtattacacc caaaatggtg tggctgtcat ggccgtctgc tgtggtccca ccaaagtgtt
     3061 tgtcgatcgg gaactgactg ccttcctggc atacggccaa acctttgccg atcgcctgcg
     3121 tcgtgccccc tgccaatcga cggccgtctg cggcaagaat atagttaatg ccatcgagag
     3181 atcggagaac ggacagatct ggattgccga caagggcggc ctggagtcgg tggcccttca
     3241 ctggtattgg catatggccg atcagtttgt gaactatatg cagagcactg acgatgagga
     3301 tcaggagttt tttttgggcc agcgatgagg catgccttga tccacggaat aaaacatcaa
     3361 atgtgtcaca aaaagtaggc aacttgtttg tgggtctgga aatagatttg aaatattttg
     3421 aggtcatatg aataatgaca taaatgaggg tgcctgaaag tatgccaagt tt
//