From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Streptomyces griseus histidine ammonia-lyase (hutH) gene, complete
Date: 27 Jan 93 06:02:27 GMT
Distribution: bionet
Checksum: 50127 6

LOCUS       STMHUTH      2946 bp ds-DNA             BCT       26-JAN-1993
DEFINITION  Streptomyces griseus histidine ammonia-lyase (hutH) gene, complete
            cds; ORF, partial cds.
ACCESSION   M77841
KEYWORDS    histidase; histidine ammonia-lyase; nonoxidative deamination.
SOURCE      Streptomyces griseus (library: lambda EMBL4; NRRL-B-2682) DNA.
  ORGANISM  Streptomyces griseus
            Prokaryotae; Firmicutes; Streptomycetaceae.
REFERENCE   1  (bases 1 to 2946)
  AUTHORS   Wu,P.-C., Kroening,T.A., White,P.J. and Kendrick,K.E.
  TITLE     Histidase from Streptomyces griseus: Purification of the enzyme and
            nucleotide sequence of the hutH structural gene
  JOURNAL   J. Bacteriol. 174, 1647-1655 (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     CDS             1..727
                     /partial
                     /note="ORF upstream of hutH"
                     /codon_start=2
                     /translation="IVLHGRAWGELYVARQAGQPVFDRADADFATVLAAVVASGIAQT
                     ERLEEVRKLAFTDPLTGLANRRAVDVRLDEAMERHRVDATVVSLVVCDLNGLKAVNDT
                     HGHAVGDRLLERFGSVLSLCGAMLPEALAARLGGDEFCLLTAGPPADAVVGVATELCD
                     RAAVIELGDGVACGVASTGDPIGPVRSARRLFRLADAAQYRAKAARAPGPVVAGRDGE
                     VVRLADSPPKSAHDRRRLRGNRP"
     CDS             835..2385
                     /gene="hutH"
                     /evidence="EXPERIMENTAL"
                     /product="histidine ammonia-lyase"
                     /codon_start=1
                     /translation="MDMHTVVVGTSGTTAEDVVAVARHGARVELSAAAVEALAAARLI
                     VDALAAKPEPVYGVSTGFGALASRHIGTELRAQLQRNIVRSHAAGMGPRVEREVVRAL
                     MFLRLKTVASGHTGVRPEVAQTMADVLNAGITPVVHEYGSLGCSGDLAPLSHCALTLM
                     GEGEAEGPDGTVRPAGELLAAHGIAPVELREKEGLALLNGTDGMLGMLVMALADLRNL
                     YTSADITAALSLEALLGTDKVLAPELHAIRPHPGQGVSADNMSRVLAGSGLTGHHQDD
                     APRVQDAYSVRCAPQVNGAGRDTLDHAALVAGRELASSVDNPVVLPDGRVESNGNFHG
                     APVAYVLDFLAIVAADLGSICERRTDRLLDKNRSHGLPPFLADDAGVDSGLMIAQYTQ
                     AALVSEMKRLAVPASADSIPSSAMQEDHVSMGWSAARKLRTAVDNLARIVAVELYAAT
                     RAIELRAAEGLTPAPASEAVVAALRAAGAEGPGPDRFLAPDLAAADTFVREGRLVAAV
                     EPVTGPLA"
BASE COUNT      380 a   1125 c   1054 g    387 t
ORIGIN      
        1 gatcgtgctg cacggccggg cgtggggtga gctgtacgtg gcgcggcagg ccggacagcc
       61 cgtcttcgac cgggccgacg cggacttcgc gaccgtcctc gccgccgtcg tggcctccgg
      121 gatcgcccag accgagcgcc tggaggaggt ccgcaagctg gccttcaccg atccgctgac
      181 cggtctggcg aaccgccggg cggtcgacgt ccggctcgac gaggccatgg agcgccaccg
      241 tgtcgacgcc acggtcgtca gcctcgtcgt ctgcgatctg aacggcctca aggcggtgaa
      301 cgacacccat ggccacgccg tcggcgaccg cctgctggaa cgtttcggct cggtgctctc
      361 cctgtgcggg gcgatgctgc ccgaggcgct ggcggcccgg ctgggcggcg atgagttctg
      421 cctgctgacg gcggggcccc cggccgacgc ggtggtcggc gtcgccaccg agctgtgcga
      481 ccgggcggcg gtgatcgagc tgggcgacgg ggtcgcctgc ggggtcgcct ccaccggcga
      541 cccgatcggc ccggtgcgct cggcccggcg gctgttccgg ctcgcggacg ccgcccagta
      601 ccgggccaag gcggcccgcg cgccgggccc cgtggtggcc gggcgggacg gcgaggtcgt
      661 ccggctcgcg gactccccgc cgaagtccgc gcacgaccgg cgcagactgc gcggcaaccg
      721 gccgtgagcc ggggtgcgcc cgtgccgtcg ccgtcgcggg gcgccccctc ccgtaagggg
      781 cccaccgcgg caccactagt gacatgaagg ctttcactac gtacgctgct gaatatggat
      841 atgcacactg ttgtggtggg gacgtccgga accaccgccg aggacgtcgt cgccgtggcc
      901 cgccacggcg cccgggtcga gctctccgcc gccgccgtgg aagccctggc cgccgcccgt
      961 ctcatcgtgg acgccctcgc cgccaaaccc gagccggtct acggtgtctc caccggcttc
     1021 ggcgccctcg ccagccgcca catcggcacg gaactgcggg cgcagctcca gcgcaacatc
     1081 gtccgctcgc acgcggccgg catgggcccg cgcgtcgagc gcgaggtcgt ccgcgcgctg
     1141 atgttcctcc ggctgaagac ggtcgcctcg gggcacaccg gcgtacgccc cgaggtcgcg
     1201 cagaccatgg ccgacgtgct gaacgcgggc atcacgcccg tcgtccacga atacggctcg
     1261 ctcggctgct ccggcgacct cgccccgctc tcgcactgcg cgctcaccct gatgggcgag
     1321 ggcgaagcgg aggggcccga cggcaccgtc cgcccggccg gggaactcct cgccgcgcac
     1381 ggcatcgccc cggtcgagct ccgcgagaag gagggcctgg ccctcctcaa cggcaccgac
     1441 ggcatgctcg gcatgctggt catggccctc gccgacctga ggaacctcta cacctcggcc
     1501 gacatcaccg cggccctgtc cctggaggcg ctcctcggta cggacaaggt cctcgccccc
     1561 gagctgcacg ccatccgccc gcaccccgga cagggcgtca gcgccgacaa catgtcgcgg
     1621 gtgctggccg gttcggggct gacgggccat caccaggacg acgccccccg ggtccaggac
     1681 gcctattcgg tgcgctgcgc gccccaggtc aacggcgcgg gccgcgacac cctcgaccac
     1741 gccgccctcg tcgcgggccg cgaactggcc tcctccgtcg acaatcccgt ggtcctcccc
     1801 gacggacgcg tcgagtccaa cgggaacttc cacggggcgc ccgtcgcgta cgtcctggac
     1861 ttcctcgcga tcgtcgccgc cgacctcggc tcgatctgcg agcgccgcac cgaccggctg
     1921 ctcgacaaga accgctcgca cggcctgccg ccgttcctcg cggacgacgc cggggtcgac
     1981 tcgggcctga tgatcgccca gtacacccag gccgccctgg tcagcgagat gaagcggctc
     2041 gcggtccccg cctccgccga ctccatcccg tcctccgcga tgcaggagga ccacgtctcc
     2101 atgggatggt ccgccgcccg caaactccgt acggccgtgg acaacctggc ccggatcgtc
     2161 gccgtcgagc tgtacgcggc gacccgcgcc atcgagctcc gcgccgccga gggcctcacc
     2221 ccggcccccg cctcggaggc cgtcgtggcc gcgctgcggg ccgccggcgc cgagggcccg
     2281 ggcccggacc gcttcctcgc gccggacctg gccgccgccg acacgttcgt acgggagggg
     2341 cgcctggtcg cggccgtgga gcccgtcacg gggccgctgg cctgagccgg tcccggaccc
     2401 gcgaaagagg ccccgtgccg tggcgcgggg cctctcgcgt acgcacggga ccgctcagag
     2461 cgccgggcgc gccccggatc cggagcggcg cacggagtag gtgacgaagc ctgcgccgag
     2521 gcccaacagg gctgtgccgc cgatgagata cggggtcgtg tccgggccgg gcccggtgtc
     2581 cgcgaggagc gcgccggagg cgtcgtcggg gctcccggcg ggccgttcgg cggctcgggc
     2641 ggacgcggcg gccggggcgc cctgatcgcc gtgctcaccg gtcgcgttgg ccgacgggac
     2701 gaaccagaga gcggccagca gcgtgcctgc ggcggtggcg gtcagcagtg tgcgacgagt
     2761 gacggacaca gattcgatcc ccttgcgaca accatgagtt agccccgtgg gccgatgcta
     2821 agcaaagcag cgggtcggtg gaaagtcgcg gcctcgccgg gccttacgct ccggccatga
     2881 gcacagaaga gacatccagg tttgttcgac ttcgcgtgga gatggtgctg gagatccgtc
     2941 gaccca
//