From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Zea mays tryptophan synthase beta-subunit (TSB1) mRNA, complete
Date: 31 Dec 92 01:39:07 GMT
Distribution: bionet
Checksum: 41573 4

LOCUS       MZEORP1A     1382 bp ss-mRNA            PLN       17-DEC-1992
DEFINITION  Zea mays tryptophan synthase beta-subunit (TSB1) mRNA, complete
            cds.
ACCESSION   M76684
KEYWORDS    TSB1 gene; tryptophan synthase beta-subunit.
SOURCE      Zea mays (library: R802 kernel library of R.Schmidt) endosperm, 22
            days after pollination cDNA to mRNA.
  ORGANISM  Zea mays
            Eukaryota; Plantae; Embryobionta; Magnoliophyta; Liliopsida;
            Commelinidae; Cyperales; Poaceae.
REFERENCE   1  (bases 1 to 1382)
  AUTHORS   Wright,A.D., Moehlenkamp,C.A., Perrot,G.H., Neuffer,M.G. and
            Cone,K.C.
  TITLE     The maize auxotrophic mutant orange pericarp is defective in
            duplicate genes for tryptophan synthase beta
  JOURNAL   Plant Cell 4, 711-719 (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     polyA_site      1382
                     /gene="TSB1"
     CDS             1..1170
                     /EC_number="4.2.1.20"
                     /standard_name="'orange pericarp-1/tryptophan synthase
                     beta-1'"
                     /gene="TSB1"
                     /evidence="EXPERIMENTAL"
                     /label=ORF
                     /partial
                     /product="tryptophan synthase beta-subunit"
                     /codon_start=1
                     /translation="GRFGGKYVPETLMHALTELENAFHALATDDEFQKELDGILKDYV
                     GRESPLYFAERLTEHYKRADGTGPLIYLKREDLNHRGAHKINNAVAQALLAKRLGKQR
                     IIAETGAGQHGVATATVCARFGLQCIIYMGAQDMERQALNVFRMKLLGAEVRAVHSGT
                     ATLKDATSEAIRDWVTNVETTHYILGSVAGPHPYPMMVREFHKVIGKETRRQAMHKWG
                     GKPDVLVACVGGGSNAMGLFHEFVEDQDVRLIGVEAAGHGVDTDKHAATLTKGQVGVL
                     HGSMSYLLQDDDGQVIEPHSISAGLDYPGVGPEHSFLKDIGRAEYDSVTDQEALDAFK
                     RVSRLEGIIPALETSHALAYLEKLCPTLPDGVRVVLNCSGRGDKDVHTASKYLDV"
BASE COUNT      310 a    358 c    423 g    291 t
ORIGIN      76 cM.
        1 gggaggttcg gcggcaagta cgtccccgag acgctcatgc acgcgctcac cgagctcgag
       61 aacgccttcc acgcactcgc caccgacgac gagttccaga aagaacttga cggtattctc
      121 aaggactacg ttggacgtga gagccccctt tacttcgcgg agcgcctgac ggagcactac
      181 aagcgcgctg atggcacagg cccgctcatc tacctcaaga gggaggacct caaccacaga
      241 ggcgcccaca agatcaacaa tgctgtggcg caggccctgc tcgccaagcg acttgggaag
      301 cagcgcatca ttgccgagac aggggctggc cagcacggtg tcgccaccgc caccgtgtgc
      361 gcccgtttcg ggctgcagtg catcatctac atgggcgcgc aagacatgga gaggcaggca
      421 ctcaatgttt tccggatgaa gcttcttggt gcagaggtga gggctgtaca ttctgggaca
      481 gcaaccctga aggatgctac ttcagaggct atccgtgact gggtcactaa cgtggagacc
      541 acacactata tattgggctc tgttgctggc ccgcacccgt acccgatgat ggtgagggag
      601 ttccacaagg ttatcggcaa ggagacccgg aggcaggcca tgcacaagtg gggtggcaag
      661 ccggacgtct tggtcgcttg cgtcgggggt ggatcaaacg ccatgggcct gttccacgag
      721 ttcgttgagg atcaggatgt gaggctgatc ggagtggagg cagctggcca tggcgtagac
      781 accgacaagc acgcggcgac tttgacaaag ggccaagttg gagttctcca tggatctatg
      841 agctatttgc tgcaggacga cgatgggcaa gttatcgagc cccactccat cagtgctgga
      901 ctggattacc ctggcgttgg acctgagcat agtttcttga aggacatcgg acgcgctgaa
      961 tatgacagcg tgactgatca ggaggcactg gatgctttca agcgcgtctc ccgtctggag
     1021 ggcatcatcc cggccctgga gacctcccac gcgctcgcct acctggagaa gctgtgcccg
     1081 accctgccgg acggcgtgag ggtggtgctg aactgcagtg ggcgaggcga caaggacgtg
     1141 cacaccgcta gcaagtacct ggacgtctag aacaccggag aggagggctg aatgagagtt
     1201 ttgcatgcca atttttgaag ggttcagcag ttatgtagcg ctgttttttt tgtgataaga
     1261 gtacaaataa cctggaagct aaagctagta ttgttgtagc cgccctttgt tctttttttg
     1321 agttgttgtt ctgtatctga aatgttttgg agtgcataat gatcaataaa caagtaaata
     1381 cc
//