From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: spidroin 2=dragline silk fibroin [Nephila clavipes=spiders, mRNA
Date: 3 Jan 93 22:20:06 GMT
Distribution: bionet
Checksum: 06552 5

LOCUS       S113892      1986 bp    mRNA            UNA       24-NOV-1992
DEFINITION  spidroin 2=dragline silk fibroin [Nephila clavipes=spiders, mRNA
            Partial, 1986 nt]
ACCESSION   S45372 M92913
KEYWORDS    .
SOURCE      spiders
  ORGANISM  Nephila clavipes
            Unclassified.
REFERENCE   1  (bases 1 to 1986)
  AUTHORS   Hinman,M.B. and Lewis,R.V.
  TITLE     Isolation of a clone encoding a second dragline silk fibroin.
            Nephila clavipes dragline silk is a two-protein fiber.
  JOURNAL   J. Biol. Chem. 267, 19320-19324 (1992)
  STANDARD  full automatic
COMMENT     This entry [NCBI gibbsq 113892] was created by the journal scanning
            component of NCBI/GenBank at the National Library of Medicine.
            This sequence comes from Fig. 1.
FEATURES             Location/Qualifiers
     CDS             1..1884
                     /partial
                     /note="dragline silk fibroin;  For the protein sequence
                     (NCBI gibbsq 113893):  Method: conceptual translation
                     supplied by author.  This sequence comes from Fig. 1."
                     /product="spidroin 2"
                     /codon_start=1
                     /translation="PGGYGPGQQGPGGYGPGQQGPSGPGSAAAAAAAAAAGPGGYGPG
                     QQGPGGYGPGQQGPGRYGPGQQGPSGPGSAAAAAAGSGQQGPGGYGPRQQGPGGYGQG
                     QQGPSGPGSAAAASAAASAESGQQGPGGYGPGQQGPGGYGPGQQGPGGYGPGQQGPSG
                     PGSAAAAAAAASGPGQQGPGGYGPGQQGPGGYGPGQQGPSGPGSAAAAAAAASGPGQQ
                     GPGGYGPGQQGPGGYGPGQQGLSGPGSAAAAAAAGPGQQGPGGYGPGQQGPSGPGSAA
                     AAAAAAAGPGGYGPGQQGPGGYGPGQQGPSGAGSAAAAAAAGPGQQGLGGYGPGQQGP
                     GGYGPGQQGPGGYGPGSASAAAAAAGPGQQGPGGYGPGQQGPSGPGSASAAAAAAAAG
                     PGGYGPGQQGPGGYAPGQQGPSGPGSASAAAAAAAAGPGGYGPGQQGPGGYAPGQQGP
                     SGPGSAAAAAAAAAGPGGYGPAQQGPSGPGIAASAASAGPGGYGPAQQGPAGYGPGSA
                     VAASAGAGSAGYGPGSQASAAASRLASPDSGARVASAVSNLVSSGPTSSAALSSVISN
                     AVSQIGASNPGLSGCDVLIQALLEIVSACVTILSSSSIGQVNYGAASQFAQVVGQSVL
                     SAF"
BASE COUNT      553 a    518 c    586 g    329 t
ORIGIN      
        1 cctggaggat atggaccagg acaacaaggc ccaggaggat atggccctgg acaacaagga
       61 ccatctggac ctggcagtgc cgctgcagca gcagcagccg ccgcagcagg acctggagga
      121 tatggccctg gacaacaagg acccggagga tatggaccag gacaacaagg acccggaaga
      181 tatggaccag gacaacaagg accatctgga cctggcagtg ccgctgcagc cgcagcagga
      241 tctggacaac aaggcccagg aggatatgga ccacgtcaac aaggtccagg aggttatgga
      301 caaggacaac aaggaccatc tggaccaggc agtgcagccg cagcctcagc cgcagcctca
      361 gcagaatctg gacaacaagg cccaggaggt tatggaccag gtcaacaagg cccaggaggt
      421 tatggaccag gtcaacaagg tcctggagga tatggaccag gacaacaagg accatctgga
      481 ccaggtagtg ccgctgcagc agccgccgcc gcatcaggac ctggacaaca aggaccagga
      541 ggatatggac caggtcaaca aggtcctgga ggatatggac caggacaaca aggaccatct
      601 ggaccaggta gtgccgctgc agccgccgcc gccgcatcag gacctggaca acaaggacca
      661 ggaggatatg gaccaggtca acaaggtcca ggaggttatg gaccaggaca acaaggacta
      721 tctggaccag gcagtgcagc tgcagcagcc gcagcaggac ctggacaaca aggacccgga
      781 ggatatggac caggacaaca aggaccatct ggacccggta gtgccgctgc agcagcagcc
      841 gccgcagcag gacctggagg atatggccct ggacaacaag gacccggagg atatggacca
      901 ggacaacaag gaccatctgg agcaggcagt gcagcagcag cagccgcagc aggacctgga
      961 caacaaggat taggaggtta tggaccagga caacaaggtc caggaggata tggaccagga
     1021 caacaaggtc caggaggata tggaccaggt agtgcatctg cagcagcagc cgcagcagga
     1081 cctggacaac aaggaccagg aggatatgga cctggacaac aaggaccatc tggaccaggc
     1141 agtgcatctg cagcagcagc cgcagccgca gcaggaccag gaggatatgg accaggacaa
     1201 caaggtccag gaggatatgc accaggacaa caaggaccat ctggaccagg cagtgcatct
     1261 gcagcagcag ccgcagccgc agcaggacca ggaggatatg gaccaggaca acaaggtcca
     1321 ggaggatatg caccaggaca acaaggacca tctggaccag gcagtgcagc agcagcagca
     1381 gctgctgcag caggacctgg tggatatgga ccagcgcaac agggaccatc tggtcctgga
     1441 atcgcagctt cagctgcttc agcaggacct ggaggttatg gaccagcaca acaaggacca
     1501 gctggatatg ggcctggaag cgcagtagca gcctctgccg gtgcaggatc tgcaggttat
     1561 gggccaggtt ctcaagcttc cgctgcagct tctcgtctgg cttctccaga ttcaggcgct
     1621 agagttgcat cagctgtttc taacttggta tccagtggcc caactagctc tgctgcctta
     1681 tcaagtgtta tcagtaacgc tgtgtctcaa attggcgcaa gtaatcctgg tctctctggt
     1741 tgcgatgtcc tcattcaagc tctcttggaa atcgtttctg cttgtgtaac catcctttct
     1801 tcatccagca ttggtcaagt taattatgga gcggcttctc agttcgccca agttgtcggc
     1861 caatctgttt tgagtgcatt ttaattgaaa aatttattaa aatatgcatg gattttctag
     1921 cctgggcaac taattgctcg tactatgtaa ttttttttta aataaattct ttgcaacttc
     1981 taaaaa
//