From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: LCB1=required for biosynthesis long-chain base component of
Date: 29 Dec 92 01:29:26 GMT
Distribution: bionet
Checksum: 59795 6

LOCUS       S41236       2470 bp    DNA             NLM       08-DEC-1992
DEFINITION  LCB1=required for biosynthesis long-chain base component of
            sphingolipids [Saccharomyces cerevisiae, Genomic, 2470 nt]
ACCESSION   S41236 M63674
KEYWORDS    .
SOURCE      Saccharomyces cerevisiae
  ORGANISM  Saccharomyces cerevisiae
            Unclassified.
REFERENCE   1  (bases 1 to 2470)
  AUTHORS   Buede,R., Rinker-Schaffer,C., Pinto,W.J., Lester,R.L. and
            Dickson,R.C.
  TITLE     Cloning and characterization of LCB1, a Saccharomyces gene required
            for biosynthesis of the long-chain base component of sphingolipids.
  JOURNAL   J. Bacteriol. 173, 4325-4332 (1991)
  STANDARD  full automatic
COMMENT     This entry [NCBI gibbsq 41236] was created by the journal scanning
            component of NCBI/GenBank at the National Library of Medicine.
            This sequence comes from Figure 4.  The authors begin their
            numbering at -333.  Zero is not present in the numbering.
FEATURES             Location/Qualifiers
     CDS             334..2010
                     /note="Description: required for biosynthesis long-chain
                     base component of sphingolipids;  For the protein sequence
                     (NCBI gibbsq 41237):  This sequence comes from Figure 4."
                     /gene="LCB1"
                     /codon_start=1
                     /translation="MAHIPEVLPKSIPIPAFIVTTSSYLWYYFNLVLTQIPGGQFIVS
                     YIKKSHHDDPYRTTVEIGLILYGIIYYLSKPQQKKSLQAQKPNLSPQEIDALIEDWEP
                     EPLVDPSATDEQSWRVAKTPVTMEMPIQNHITITRNNLQEKYTNVFNLASNNFLQLSA
                     TEPVKEVVKTTIKNYGVGACGPAGFYGNQDVHYTLEYDLAQFFGTQGSVLYGQDFCAA
                     PSVLPAFTKRGDVIVADDQVSLPVQNALQLSRSTVYYFNHNDMNSLECLLNELTEQEK
                     LEKLPAIPRKFIVTEGIFHNSGDLAPLPELTKLKNKYKFRLFVDETFSIGVLGATGRG
                     LSEHFNMDRATAIDITVGSMATALGSTGGFVLGDSVMCLHQRIGSNAYCFSACLPAYT
                     VTSVSKVLKLMDSNNDAVQTLQKLSKSLHDSFASDDSLRSYVIVTSSPVSPVLHLQLT
                     PAYRSRKFGYTCEQLFETMSALQKKSQTNKFIEPYEEEEKFLQSIVDHALINYNVLIT
                     RNTIVLKQETLPIVPSLKICCNAAMSPEELKNACESVKQSILACCQESNK"
BASE COUNT      711 a    587 c    490 g    682 t
ORIGIN      
        1 cgcgtatttt tttttttttg aggcgccatg atttcttaca cggtttcttt tttttttcct
       61 tctttccttc ttgcttctct gctaacaaat ttttcactca ttctttttta taggggcata
      121 ttgctgcggt taactgtagt gaacgaaagt aagattgaga aaatatagta cttaagaaaa
      181 agaaaaggaa aaataaaaaa aattcttttc aacatcatcg agtagcacag tataagagcg
      241 ctctaacctt ctgcctggcc tccaatatac acattttgct cgtgtagggt tatttatcct
      301 tttttcttcc ttcccaccca aaaaaaaaaa gcaatggcac acatcccaga ggttttaccc
      361 aaatcaatac cgattccggc atttattgtt accacctcat cgtacctatg gtactacttc
      421 aatctggtgt tgactcaaat cccgggaggc caattcatcg tttcgtacat caagaaatcg
      481 catcatgacg atccatacag gaccacggtt gagatagggc ttattttata cgggatcatc
      541 tattacttgt ccaagccaca acagaaaaag agtcttcaag cacagaagcc caacctatcg
      601 ccccaggaga ttgacgcgct aattgaggac tgggagcccg agcctctagt cgacccttct
      661 gccaccgatg agcaatcgtg gagggtggcc aaaacacccg tcaccatgga aatgcccatt
      721 cagaaccata ttactatcac cagaaacaac ctgcaggaga agtataccaa tgttttcaat
      781 ttggcctcga acaacttttt gcaattgtcc gctacggagc ccgtgaaaga agtggtcaag
      841 accactatca agaattacgg tgtgggcgcc tgtggtcccg ccgggttcta cggtaaccag
      901 gacgttcatt acacgttgga atatgattta gcacagttct ttggcaccca aggttccgtt
      961 ctgtacgggc aagacttttg tgccgcaccc tctgttctgc ctgctttcac aaagcgtggt
     1021 gatgttatcg tggcagacga ccaggtgtca ttaccagtgc aaaatgctct gcaactaagc
     1081 agatccacag tctactactt caaccacaac gatatgaatt cgctagaatg tttattaaac
     1141 gagttgaccg aacaggagaa acttgagaaa ctgcccgcca ttccaagaaa atttatcgtc
     1201 actgagggta ttttccacaa ctcgggcgat ttagctccgt tgcctgagtt gactaagctg
     1261 aagaacaagt acaagttcag actatttgtt gacgaaacct tctccattgg tgttcttggc
     1321 gctacgggcc gtgggttgtc agagcacttc aacatggatc gcgcaactgc cattgacatt
     1381 accgttgggt ccatggccac cgcgttgggg tccaccggtg gttttgtcct gggtgacagt
     1441 gttatgtgtt tgcaccagcg tattggttcc aatgcatatt gtttttctgc ctgtttgccg
     1501 gcttacaccg tcacatccgt ctccaaagtc ttgaaattga tggactccaa caacgacgcc
     1561 gtccagacgc tgcaaaaact atccaaatct ttgcatgatt cctttgcatc tgacgactcc
     1621 ttgcgttcat acgtaatcgt cacgtcctct ccagtgtctc ctgtcctaca tctgcaactg
     1681 actcccgcat ataggtctcg caagttcgga tacacctgcg aacagctatt cgaaaccatg
     1741 tcagctttgc aaaagaagtc ccagacaaac aaattcattg agccatacga agaggaggaa
     1801 aaatttctgc agtccatagt agatcatgct cttattaact acaacgttct catcacaaga
     1861 aacactattg ttttaaaaca ggagacgcta ccaattgtcc ctagcttgaa aatctgctgt
     1921 aacgccgcca tgtccccaga ggaactcaaa aatgcttgcg aaagtgtcaa gcagtccatc
     1981 cttgcctgtt gccaagaatc taataaataa aaatagaaag ccagtatatg cacacgcaca
     2041 tatatatata aatatttata caataataca aataatcgta acatcatctc tgtcaaattg
     2101 acgtggtgca cggcgcccag agaatgcgct aaaaattttc ggatccgaaa ttttctttcc
     2161 ttttaccatc gaggcaaagc aacctgtatt atttatttgt ttatttatta atagaaaaga
     2221 aaggagtact ttcgtggtac gctttcttga gcattttcgg tttcactagg cagagaacta
     2281 acacaagaga cacagcaaac atcaaacaag gttaaaacag cacaccaagg caatatgatg
     2341 cattttagaa agaaatccag tatcagtaac acgagtgatc atgacggagc gaaccgtgcc
     2401 tcagatgtca agatttctga agatgacaag gcaagattga agatgcgtac tgcttccgtt
     2461 gctgatccta
//