From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: D.pseudoobscura Adh gene for alcohol dehydrogenase
Date: 28 Dec 92 21:26:57 GMT
Distribution: bionet
Checksum: 05286 8

LOCUS       DROADHGN30   3454 bp ds-DNA             INV       01-DEC-1992
DEFINITION  D.pseudoobscura Adh gene for alcohol dehydrogenase
ACCESSION   X62210
KEYWORDS    ADH gene; alcohol dehydrogenase.
SOURCE      Drosophila pseudoobscura DNA.
  ORGANISM  Drosophila pseudoobscura
            Eukaryota; Animalia; Metazoa; Arthropoda; Uniramia; Insecta;
            Pterygota; Neoptera; Holometabola; Diptera; Brachycera;
            Cyclorrhapha; Schizophora; Drosophiloidea; Drosophilidae.
REFERENCE   1  (bases 1 to 3454)
  AUTHORS   Schaeffer,S.W. and Miller,E.L.
  TITLE     Estimates of gene flow in Drosophila pseudoobscura determined from
            nucleotide sequence analysis of the alcohol dehydrogenase region
  JOURNAL   Genetics 132, 471-480 (1992)
  STANDARD  full automatic
COMMENT     *source: strain=GB68; *source: isolate=Gundlach Bundschu Winery,
            Sonoma Valley, *source: California; *source: chromosome=IV section
            88;
               From EMBL    entry DPADHGN30;  dated 16-OCT-1992.
FEATURES             Location/Qualifiers
     mRNA            join(40..119,912..1017,1081..1485,1548..1963)
                     /note="adult mRNA (Adh)"
     mRNA            join(879..1017,1081..1485,1548..1963)
                     /note="larval mRNA (Adh)"
     promoter        8..14
                     /note="adult promoter (Adh)"
     prim_transcript 40..1963
                     /note="adult primary transcript (Adh)"
     exon            40..119
                     /note="adult exon 1a"
     intron          120..911
                     /note="adult intron"
     promoter        843..850
                     /note="larval promoter (Adh)"
     prim_transcript 879..1963
                     /note="larval primary transcript (Adh)"
     exon            879..1017
                     /note="larval exon 1"
     exon            912..1017
                     /note="adult exon 1b"
     intron          1018..1080
                     /number=1
     exon            1081..1485
                     /number=2
     intron          1486..1547
                     /number=2
     exon            1548..1963
                     /number=3
     polyA_signal    1940..1945
     polyA_site      1963
     CDS             join(925..1017,1081..1485,1548..1814)
                     /EC_number="1.1.1.1"
                     /gene="Adh"
                     /product="alcoholdehydrogenase"
                     /codon_start=1
                     /translation="MSLTNKNVVFVAGLGGIGLDTSRELVKRNLKNLVILDRIDNPAA
                     IAELKAINPKVTITFYPYDVTVPVAETTKLLKTIFAQVKTIDVLINGAGILDDHQIER
                     TIAVNYTGLVNTTTAILDFWDKRKGGPGGIICNIGSVTGFNAIYQVPVYSGSKAAVVN
                     FTSSLAKLAPITGVTAYTVNPGITKTTLVHKFNSWLDVEPRVAEKLLEHPTQTSQQCA
                     ENFVKAIELNKNGAIWKLDLGTLEPITWTQHWDSGI"
BASE COUNT      965 a    791 c    788 g    910 t
ORIGIN      
        1 cgcagtgtat ttaaggcact gcacttatcg aggaccacga ttattgtcta cagtgcagtc
       61 agtagttgtc aggtgcagca ggcggaccag catttcttta aatctactca atcgatcaag
      121 taagtaactg taatccaagt acagtcggcc catcccactc ggttctgccg tagctcaaga
      181 aatttcttga tagcgcagtt ccagttgtag aacgtcgttt aaaaaaacat ctcgttggag
      241 cttgggttga aaatgttcga gttgggcgta gaaaatttca aatataaaat atcaaaaaag
      301 agaaaatatc ttcccaaagc agctgtccat tttttgttaa agtgatagat caaaaattcg
      361 tatgacgtat ttgccaagtg ctctttatgc aaataatttc ctcaattcta attctacaag
      421 aagcactttt ggctcttgtc cagaaaaaac aatttgttta cacttttcgg ttgataacag
      481 aggcggaccg acggctaggt aacataacag tcgcctgtta agtaatgccc tcactctctg
      541 ttagatggcc gcgcgtgtcg actccgatag accagcagac acgcagtagt tttcctcaag
      601 aacctgaact ctaaacatag acataattta cgataccttg agactcaaat aaaaatacaa
      661 gtatgagtgg tattctattc ctgattagac tcgcacacac acgtacgagt attaacataa
      721 atttgccggc aaataaccaa aacaaaacga acgcaaacga tccgaacacg taatgcgaga
      781 gataagaaac agaaaagctc gacgtgaaag aagcttctgg cggaagctct tgccgaactc
      841 cctataaata cgagacagaa aaccagaata aatataataa caaactgttg aattgtccca
      901 tcgattccca gatcaaaaga cagaatgtct ctcaccaaca agaacgtcgt tttcgtggcc
      961 ggtctgggcg gcattggcct ggacaccagc cgggagttgg tcaagcgtaa tctgaaggta
     1021 agagagaacg aattccatgg aatctatgga atcctaaatt taaaaattca tttattttag
     1081 aacctggtca tcctggatcg cattgacaat ccggctgcca ttgccgaact gaaggcaatc
     1141 aatcccaagg tgaccatcac cttctatccc tacgatgtga ctgtgcccgt cgctgagacc
     1201 accaagctcc tgaagaccat ctttgcccag gtgaagacaa tcgatgtcct gatcaacggt
     1261 gctggcatcc tggacgatca tcagatcgag cgcaccattg ccgttaacta cacgggcctg
     1321 gtcaacacca ccacagccat tctggacttc tgggacaagc gcaagggcgg cccaggcggc
     1381 atcatttgca acattggctc cgtcaccggt ttcaatgcca tctaccaggt gcccgtttac
     1441 tccggctcca aggcggcggt ggttaacttt acctcctccc tggcggtaag taatcctaca
     1501 actatcccaa aatccccaaa actaaatatt tctaaatatt cccatagaaa ctggctccca
     1561 ttactggtgt cactgcttac actgtcaatc ctggcatcac taagaccact ctggtccaca
     1621 aattcaactc gtggctggat gtggagcccc gtgtggcgga gaagctgctc gagcatccca
     1681 cccagacctc tcagcagtgc gccgagaact ttgtgaaggc cattgagctg aacaagaacg
     1741 gtgccatctg gaagttggat ttgggcacct tggagcccat cacatggacc cagcactggg
     1801 actcgggcat ctaaaccatc ccagagactc tatgggacat ggcgttttag cttttagttt
     1861 ctttttttcc actcaattgt tacgtatata catacacata tagcaataag gctgatttga
     1921 accccgtttt gaatatgata atacaaaatt atatttgaga aatttcaaca aaatcgataa
     1981 gagctctagc tctcttttaa ttaaaaaaaa ctagataaaa agagcaatgg tcagtggtcg
     2041 ttgtggcctc tatcgatttc cacacaaaaa ctttactgtt tagtaataga aaagaacttg
     2101 agaaaggcag ccaaaagaat gtacgatctg acgggtaagc atgtctgcta tgtggctgac
     2161 tgcggtggca ttgcactgga gaccagcaag gttctcatga ccaagaatat agcggtgagt
     2221 ggagagtgga gagtgttccg aagtggttct ggttcttaaa caagtctcta gtctctagtc
     2281 tctagtctct tggctgctgc tggtagctgt cgaaactaat taagttaatg actttttcat
     2341 gttttgtttt tgtccgctga cttttgctgt tcaaagctac aattaagttt atgttttgag
     2401 tttgatctac ttttgcttaa gggaagtact ttcgattacg gattgctggc atataaaaat
     2461 agataccaag gaatgtgcat catattgatt ctatcttgat tctttgccca gaaactggcc
     2521 atccttcaga gcgtggaaaa ccccccggcc atcgcccagt tacaatcgat taagcacagc
     2581 acacagatct tcttctggac cttcgatgtg accatggccc gcgaggagat gaagaagtac
     2641 ttcgatgagg tcatggtcca gatggactac atcgatgtgc tgatcaatgg ggccaccctg
     2701 tgcgatgagc ggaacattga tgccaccatc aatacgaatc tgaccggcat gatgaacacc
     2761 gtggccactg tgctgcccta tatggaccgt aagatgggcg gttcgggtgg actgattgtg
     2821 aatgtcacct ccgtcatagg actggatcca tcgccggtct tttgtgcgta cagtgcctca
     2881 aagtttggtg tgattgggtt cacccgaagt ctagcggtga gtgggcgaga tcctaacttg
     2941 aaaatgctct ctaatgtctt ccattctttc gcaggatccc ttgtattaca cccaaaatgg
     3001 tgtggctgtc atggccgtct gctgtggtcc caccaaagtg tttgtcgatc gggaactgac
     3061 tgccttcctg gaatacggcc agtcctttgc cgatcgcctg cgtcgtgccc cctgccaatc
     3121 gacggccgtc tgcggccaga atatagtaaa tgccatcgag agatcggaga acggacagat
     3181 ctggattgcc gacaagggcg gcctggagtc ggtggccctc cactggtatt ggcatatggc
     3241 cgatcagttt gtgaactata tgcagagcac tgacgatgag gatcaggagt tttttttggg
     3301 gcagcgatga ggcatgcctt gatccacgga ataaaacatc aaatgtgtca caaaaagtag
     3361 gcaacttgtt tgtgggtttg gaaatagatt tgaaatattt tgaggtcata tgaataatga
     3421 cataaatgag ggtgcctgaa agtatgccaa gttt
//