From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: E.coli 177 kD protein involved in chromosome partitioning (mukB)
Date: 22 Dec 92 22:51:17 GMT
Distribution: bionet
Checksum: 50674 10

LOCUS       ECOMUKB      5353 bp ds-DNA             BCT       30-NOV-1992
DEFINITION  E.coli 177 kD protein involved in chromosome partitioning (mukB)
            gene, complete cds.
ACCESSION   M63930 M38402 X57550
KEYWORDS    mukB gene.
SOURCE      E.coli K-12 DNA.
  ORGANISM  Escherichia coli
            Prokaryotae; Gram-negative facultatively anaerobic rods;
            Enterobacteriaceae.
REFERENCE   1  (bases 1 to 5353)
  AUTHORS   Niki,H., Jaffe,A., Imamura,R., Ogura,T. and Hiraga,S.
  TITLE     The new gene mukB codes for a 177 kDa protein with coiled-coil
            domains involved in chromosome partitioning of E.coli
  JOURNAL   EMBO J. 10, 183-193 (1991)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     -35_signal      392..397
                     /gene="mukB"
     -10_signal      415..421
                     /gene="mukB"
     RBS             448..451
                     /gene="mukB"
     CDS             459..5063
                     /function="involved in chromosome partitioning"
                     /gene="mukB"
                     /codon_start=1
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISERTNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELADDNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQQENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADCAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFTS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQVRTVKRRLRVLSADAARCPSAGVCLTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLDRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMVQDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLNPQ
                     IDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGCCAQSLVHCRPVRRWYRYVDSGVV
                     VQSWEDESAACAVKISLLAACCSSMKQRDWMLVLSPRCLNCVSVCKCNSSSQRRKISA
                     RRKAPPINWCVKSSRIPNTFMSSACEDLRRNSLKRFQELTKRLLRRVKIKQQCRLFFF
                     RKLRFCTKKVAHYGALFFKLLYIRLCKNVRRLYTEDKPDE"
BASE COUNT     1279 a   1369 c   1564 g   1141 t
ORIGIN      
        1 gatccctcgt tccgtcttgt cggaactgga tatgatggtc gggaaaatcc tctgttatct
       61 ctatctacgc cggaacggct ggcgaatgag gggattttca cccagcagga actgtacgac
      121 gaactgctca ccctggccga tgaagcaaaa ctgctgaaac tggtgaacaa ccgttcaacc
      181 ggttcagacg ttgaccgtca gaagttgcag gagaaagtac gttcttcgct caaccgtctg
      241 cgtcgtttag gcatggtgtg gtttatgggc cacgacagca gcaagtttcg cattaccgaa
      301 tcggtgttcc gcttcggagc cgatgtgcgt gctggcgacg atccccgtga agcacagcgt
      361 cgcctgattc gtgatggcga agcaatgccg attgaaaatc atctgcaact caacgatgaa
      421 accgaagaga atcagccaga tagcggagag gaagaataat gattgaacgc ggtaaatttc
      481 gctcactgac gctgattaac tggaacggct tttttgcccg aacttttgac cttgacgagc
      541 tggtcacgac gctttctggc ggtaacgggg cgggtaaatc caccaccatg gcggcgttcg
      601 ttacggcgct gatccccgac ctgaccctgc tgcatttccg taacactacg gaagccgggg
      661 ccaccagcgg ttcgcgcgat aaaggtctgc acggtaagct gaaagcgggt gtctgttatt
      721 cgatgctcga caccattaac tcgcgccacc agcgcgtggt ggtcggtgtg cgtctgcaac
      781 aggttgccgg acgcgatcgt aaagtggata tcaagccgtt tgccattcag ggactgccga
      841 tgtcggtgca gccgacacag ctggtgaccg aaaccctgaa cgaacgccag gcgcgcgtgc
      901 tgccgcttaa cgagctgaaa gacaagctcg aggcgatgga aggcgtgcag tttaaacagt
      961 tcaactccat tactgattac cactcgctga tgttcgatct gggcatcatc gcgcgtcgtc
     1021 tgcgctccgc atctgaccgt agcaaattct atcgtctgat cgaagcttcg ctgtatggcg
     1081 ggatctccag tgccattacc cgttctctgc gcgactacct gttgccagaa aacagcggcg
     1141 tgcgtaaagc gttccaggac atggaagcgg cgctgcgtga aaaccgtatg acgctggaag
     1201 cgattcgtgt cacccagtcg gaccgcgacc tgtttaagca tctgatcagc gaacgcacca
     1261 actacgtggc ggcggactac atgcgtcacg ccaacgagcg ccgtgtccat ctcgacaaag
     1321 ccctggagtt tcgtcgcgag ctacatactt cgcgtcagca actggcggct gagcagtaca
     1381 aacacgtcga tatggcgcgt gagctggcag acgacaacgg tgccgaaggc gatctggaag
     1441 cggattatca ggcggccagt gatcacctga acctggtgca aaccgcactg cgtcagcagg
     1501 agaagatcga acgctacgaa gcggatctcg atgagctgca gatccgtctg gaagagcaaa
     1561 atgaagtggt ggcagaagcc atcgaacgcc agcaagagaa tgaggctcgt gcggaagctg
     1621 ccgaactgga agtggacgag ctgaaaagcc agcttgctga ctaccagcag gcgctggacg
     1681 tccagcaaac gcgcgcgatc cagtataacc aggcgattgc tgcgcttaat cgtgccaaag
     1741 aactgtgcca tctgccggac ttaaccgccg actgcgccgc cgaatggctg gaaaccttcc
     1801 aggcgaaaga gctggaagcg actgaaaaaa tgctctctct tgagcagaaa atgagcatgg
     1861 cgcaaaccgc gcacagccag tttgagcagg cttatcagct ggtggtggca atcaacggcc
     1921 cactggcgcg taacgaggcg tgggatgtcg ctcgcgaact attgcgcgaa ggggtcgatc
     1981 agcgtcacct ggcagagcag gttcagccgc tgcggatgcg attaagcgaa ctggaacagc
     2041 gtctacgcga gcagcaagaa gctgagcgtc tgctggcaga tttctgcaaa cgtcagggca
     2101 agaattttga tatcgacgaa ctggaagccc tgcatcagga actggaagca cgcattgcct
     2161 ctctttccga tagcgtgtct aacgcccgtg aagagcgcat ggcactgcgc caggagcagg
     2221 aacagctgca gtctcgcatt cagagtttga tgcagcgtgc gccggtttgg ctggcagcgc
     2281 aaaacagtct caaccagttg agcgaacagt gcggcgaaga gtttacctcc agccaggacg
     2341 tcaccgaata tctgcaacag ttgctggagc gtgagcgtga ggcgattgtt gaacgcgatg
     2401 aagtgggcgc gcgcaaaaac gccgtcgatg aagagatcga acgtttaagc cagcctggcg
     2461 gctctgaaga tcagcgtctg aacgcgctgg cggagcgttt tggtggtgtg ctgctgtcag
     2521 aaatttatga cgacgttagc ctggaagatg cgccgtactt ctcagcgctg tatggcccgt
     2581 cacgccacgc catcgtggtg ccagatctgt cacaggtaac tgaacacctg gaaggcttga
     2641 ccgattgccc ggaagatctc tatctgatcg aaggagatcc gcagtcattc gatgacagcg
     2701 tgttcagcgt tgatgagctg gaaaaagcgg tagtggtgaa aatcgccgat cgtcagtggc
     2761 gttattcacg tttcccggaa gtgccgctgt ttggtcgtgc tgcgcgtgaa agccgtattg
     2821 aaagcctcca tgccgagcgt gaagtgcttt ccgaacgctt cgccacgctc tcctttgatg
     2881 tacagaaaac tcagcgtctg catcaggcgt tcagccgctt tatcggcagt catctggcgg
     2941 ttgcgtttga gtctgacccg gaagcagaaa tccgtcaact gaacagccgt cgcgtcgaac
     3001 tggagcgggc gttaagtaat catgaaaatg ataaccagca gcagcgtatt cagtttgagc
     3061 aggcgaaaga gggcgttacg gcgctgaacc gcattctgcc gcgtctcaac ctgttggctg
     3121 atgacagcct ggcggatcgc gtcgatgaaa tccgcgaacg tctggatgaa gcccaggaag
     3181 ccgcgcgttt tgttcagcag tttggcaatc aactggcgaa actggaaccg attgtttcgg
     3241 tattgcagag cgacccggaa caggttcgaa cagttaaaag aagattacgc gtactctcag
     3301 cagatgcagc gcgatgcccg tcagcaggcg tttgcctgac ggaagtggtg cagcgtcgtg
     3361 cgcactttag ctattctgac tcggcagaaa tgcttagcgg taacagcgat ctcaacgaaa
     3421 aactgcgtga acgtctggaa caggcggaag cggagcgtac ccgtgctcgc gaagcgttgc
     3481 gcggtcacgc agcgcagttg agtcagtaca accaggtgct ggcttcgctg aaaagttctt
     3541 acgacaccaa aaaagagcta ctcaacgatc tgcaacgtga attgcaggat atcggcgtgc
     3601 gtgctgatag cggggcagaa gagcgggcgc gtattcgccg tgacgagctg catgcgcaac
     3661 tgagcaataa ccgttcacgc cgcaatcaac tggaaaaagc gcttaccttc tgcgaagcgg
     3721 agatggacaa cctgacccgc aaactgcgca agctggagcg ggattacttt gagatgcgcg
     3781 agcaggtagt gaccgccaaa gcgggctggt gtgcggtgat gcgcatggtg aaagataacg
     3841 gcgttgagcg ccgcttagac cgtcgtgagc tggcttatct ctccgctgat gatttgcgtt
     3901 ccatgtcgga taaggcgtta ggtgcgctgc gtctggcggt ggcggataac gaacatctgc
     3961 gcgacgtgct gcgcatggtg caagatccga aacgtccgga gcgtaaaatt cagttcttcg
     4021 tggcggttta tcagcatctg cgtgaacgta ttcgtcagga tattattcgt accgatgatc
     4081 cggtggaagc tatcgaacag atggagattg aacttagccg tctgaccgaa gaattaacct
     4141 cccgtgaaca gaaactggcg atcagttccc gcagcgtggc gaacatcatt cgcaaaacca
     4201 ttcagcgcga gcagaaccgt atccgtatgc tcaaccaggg gttgcagaac gtatcgtttg
     4261 gtcaggtgaa cagcgtgcgt ctcaacgtgc gtgaaacgca cgccatgcta ctggatgtgc
     4321 tctctgaaca gcacgagcag catcaggatc tgtttaacag caaccgtttg accttctcgg
     4381 aagcgctggc gaaactgtat caacgtctta acccgcagat tgatatgggg cagcgcacgc
     4441 cgcagaccat cggtgaagaa ctgctggatt accgcaacta tctggaaatg gaagttgagg
     4501 ttaaccgtgg ttccgatggc tgctgcgcgc agagtctggt gcattgtcga ccggtgaggc
     4561 gttggtaccg gtatgtcgat tctggtgtgg tggtacaaag ctgggaagat gaatctgccg
     4621 cctgcgcggt aaagatatct ctccttgccg cctgctgttc ctcgatgaag cagcgcgact
     4681 ggatgctcgt tctatcgcca cgctgtttga attgtgtgag cgtttgcaaa tgcaactcat
     4741 catcgcagcg ccggaaaata tcagcccgga gaaaggcacc acctataaac tggtgcgtaa
     4801 agtcttccag aataccgaac acgttcatgt cgtcggcctg cgaggatttg cgccgcaact
     4861 ccctgaaacg cttccaggaa ctgacgaagc gccttctcag gcgagttaaa attaagcagc
     4921 aatgccgcct tttctttttc cgaaaactcc gtttctgcac taaaaaagtg gcacattacg
     4981 gcgcgctttt ctttaaactt ctttacatta ggttatgtaa aaacgtacgg cgtttatata
     5041 ctgaagataa gcctgatgag taacaggctt gctcgtcata ctttcgtgag tattggcgtt
     5101 gtacaggcaa gtcgtaaaat aacagcctgg ctattcaggt atgataaaaa cagggggcaa
     5161 gggatgttgc ttaatatgat gtgtggtcgt cagctgtcgg caatcagttt gtgcctggcc
     5221 gtaacattcg ctccactgtt caatgcgcag gccgatgagc ctgaagtaat ccctggcgaa
     5281 cgcccggtgg ctgtcagtga acagggcgag gcactgccgc aggcgcaagc cacggcaata
     5341 atggcgggga tcc
//