From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human enkephalinase (MME) mRNA, complete cds.
Date: 5 Nov 92 23:10:38 GMT
Distribution: bionet
Checksum: 59107 7

LOCUS       HUMMRNAEN    3181 bp ss-mRNA            PRI       15-JUN-1989
DEFINITION  Human enkephalinase (MME) mRNA, complete cds.
ACCESSION   X07166
KEYWORDS    enkephalinase; metalloprotein; neutral endopeptidase.
SOURCE      human (Homo sapiens).
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 3181)
  AUTHORS   Malfroy,B., Kuang,W.-J., Seeburg,P.H., Mason,A.J. and
            Schofield,P.R.
  TITLE     Molecular cloning and amino acid sequence of human enkephalinase
            (neutral endopeptidase)
  JOURNAL   FEBS Lett. 229, 206-210 (1988)
  STANDARD  full automatic
COMMENT     *source: library=lambda gt10;tissue=placenta;clone=lambda H7;

              EMBL features not translated to GenBank features:
              key        from     to       description

              SITE       3073   3078       poly A signal.
FEATURES             Location/Qualifiers
     CDS             18..2249
                     /EC_number="3.4.24.11"
                     /gene="MME"
                     /product="enkephalinase"
                     /codon_start=1
                     /translation="MDITDINTPKPKKKQRWTPLEISLSVLVLLLTIIAVTMIALYAT
                     YDDGICKSSDCIKSAARLIQNMDATTEPCTDFFKYACGGWLKRNVIPETSSRYGNFDI
                     LRDELEVVLKDVLQEPKTEDIVAVQKAKALYRSCINESAIDSRGGEPLLKLLPDIYGW
                     PVATENWEQKYGASWTAEKAIAQLNSKYGKKVLINLFVGTDDKNSVNHVIHIDQPRLG
                     LPSRDYYECTGIYKEACTAYVDFMISVARLIRQEERLPIDENQLALEMNKVMELEKEI
                     ANATAKPEDRNDPMLLYNKMTLAQIQNNFSLEINGKPFSWLNFTNEIMSTVNISITNE
                     EDVVVYAPEYLTKLKPILTKYSARDLQNLMSWRFIMDLVSSLSRTYKESRNAFRKALY
                     GTTSETATWRRCANYVNGNMENAVGRLYVEAAFAGESKHVVEDLIAQIREVFIQTLDD
                     LTWMDAETKKRAEEKALAIKERIGYPDDIVSNDNKLNNEYLELNYKEDEYFENIIQNL
                     KFSQSKQLKKLREKVDKDEWISGAAVVNAFYSSGRNQIVFPAGILQPPFFSAQQSNSL
                     NYGGIGMVIGHEITHGFDDNGRNFNKDGDLVDWWTQQSASNFKEQSQCMVYQYGNFSW
                     DLAGGQHLNGINTLGENIADNGGLGQAYRAYQNYIKKNGEEKLLPGLDLNHKQLFFLN
                     FAQVWCGTYRPEYAVNSIKTDVHSPGNFRIIGTLQNSAEFSEAFHCRKNSYMNPEKKC
                     RVW"
BASE COUNT     1055 a    582 c    657 g    887 t
ORIGIN      
        1 gcaagtcaga aagtcagatg gatataactg atatcaacac tccaaagcca aagaagaaac
       61 agcgatggac tccactggag atcagcctct cggtccttgt cctgctcctc accatcatag
      121 ctgtgacaat gatcgcactc tatgcaacct acgatgatgg tatttgcaag tcatcagact
      181 gcataaaatc agctgctcga ctgatccaaa acatggatgc caccactgag ccttgtacag
      241 actttttcaa atatgcttgc ggaggctggt tgaaacgtaa tgtcattccc gagaccagct
      301 cccgttacgg caactttgac attttaagag atgaactaga agtcgttttg aaagatgtcc
      361 ttcaagaacc caaaactgaa gatatagtag cagtgcagaa agcaaaagca ttgtacaggt
      421 cttgtataaa tgaatctgct attgatagca gaggtggaga acctctactc aaactgttac
      481 cagacatata tgggtggcca gtagcaacag aaaactggga gcaaaaatat ggtgcttctt
      541 ggacagctga aaaagctatt gcacaactga attctaaata tgggaaaaaa gtccttatta
      601 atttgtttgt tggcactgat gataagaatt ctgtgaatca tgtaattcat attgaccaac
      661 ctcgacttgg cctcccttct agagattact atgaatgcac tggaatctat aaagaggctt
      721 gtacagcata tgtggatttt atgatttctg tggccagatt gattcgtcag gaagaaagat
      781 tgcccatcga tgaaaaccag cttgctttgg aaatgaataa agttatggaa ttggaaaaag
      841 aaattgccaa tgctacggct aaacctgaag atcgaaatga tccaatgctt ctgtataaca
      901 agatgacatt ggcccagatc caaaataact tttcactaga gatcaatggg aagccattca
      961 gctggttgaa tttcacaaat gaaatcatgt caactgtgaa tattagtatt acaaatgagg
     1021 aagatgtggt tgtttatgct ccagaatatt taaccaaact taagcccatt cttaccaaat
     1081 attctgccag agatcttcaa aatttaatgt cctggagatt cataatggat cttgtaagca
     1141 gcctcagccg aacctacaag gagtccagaa atgctttccg caaggccctt tatggtacaa
     1201 cctcagaaac agcaacttgg agacgttgtg caaactatgt caatgggaat atggaaaatg
     1261 ctgtggggag gctttatgtg gaagcagcat ttgctggaga gagtaaacat gtggtcgagg
     1321 atttgattgc acagatccga gaagttttta ttcagacttt agatgacctc acttggatgg
     1381 atgccgagac aaaaaagaga gctgaagaaa aggccttagc aattaaagaa aggatcggct
     1441 atcctgatga cattgtttca aatgataaca aactgaataa tgagtacctc gagttgaact
     1501 acaaagaaga tgaatacttc gagaacataa ttcaaaattt gaaattcagc caaagtaaac
     1561 aactgaagaa gctccgagaa aaggtggaca aagatgagtg gataagtgga gcagctgtag
     1621 tcaatgcatt ttactcttca ggaagaaatc agatagtctt cccagccggc attctgcagc
     1681 cccccttctt tagtgcccag cagtccaact cattgaacta tgggggcatc ggcatggtca
     1741 taggacacga aatcacccat ggcttcgatg acaatggcag aaactttaac aaagatggag
     1801 acctcgttga ctggtggact caacagtctg caagtaactt taaggagcaa tcccagtgca
     1861 tggtgtatca gtatggaaac ttttcctggg acctggcagg tggacagcac cttaatggaa
     1921 ttaatacact gggagaaaac attgctgata atggaggtct tggtcaagca tacagagcct
     1981 atcagaatta tattaaaaag aatggcgaag aaaaattact tcctggactt gacctaaatc
     2041 acaaacaact atttttcttg aactttgcac aggtgtggtg tggaacctat aggccagagt
     2101 atgcggttaa ctccattaaa acagatgtgc acagtccagg caatttcagg attattggga
     2161 ctttgcagaa ctctgcagag ttttcagaag cctttcactg ccgcaagaat tcatacatga
     2221 atccagaaaa gaagtgccgg gtttggtgat cttcaaaaga agcattgcag cccttggcta
     2281 gacttgccaa caccacagaa atggggaatt ctctaatcga aagaaaatgg gccctagggg
     2341 tcactgtact gacttgaggg tgattaacag agagggcacc atcacaatac agataacatt
     2401 aggttgtcct agaaagggtg tggagggagg aagggggtct aaggtctatc aagtcaatca
     2461 tttctcactg tgtacataat gcttaatttc taaagataat attactgttt atttctgttt
     2521 ctcatatggt ctaccagttt gctgatgtcc ctagaaaaca atgcaaaacc tttgaggtag
     2581 accaggattt ctaatcaaaa gggaaaagaa gatgttgaag aatacagtta ggcaccagaa
     2641 gaacagtagg tgacactata gtttaaaaca cattgcctaa ctactagttt ttacttttat
     2701 ttgcaacatt tacagtcctt caaaatcctt ccaaagaatt cttatacaca ttggggcctt
     2761 ggagcttaca tagttttaaa ctcatttttg ccatacatca gttattcatt ctgtgatcat
     2821 ttattttaag cactcttaaa gcaaaaaatg aatgtctaaa attgtttttt gttgtacctg
     2881 ctttgactga tgctgagatt cttcaggctt cctgcaattt tctaagcaat ttcttgctct
     2941 atctctcaaa acttggtatt tttcagagat ttatataaat gtaaaaataa taatttttat
     3001 atttaattat taactacatt tatgagtaac tattattata ggtaatcaat gaatattgaa
     3061 gtttcagctt aaaataaaca gttgtgaacc aagatctata aagcgatata cagatgaaaa
     3121 tttgagacta tttaaactta taaatcatat tgatgaaaag atttaagcac aaactttagg
     3181 g
//