From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Homo sapiens histone-binding protein mRNA, complete cds.
Date: 3 Jan 93 22:15:40 GMT
Distribution: bionet
Checksum: 11891 7

LOCUS       HUMHSTNBP    2561 bp ss-mRNA            PRI       23-DEC-1992
DEFINITION  Homo sapiens histone-binding protein mRNA, complete cds.
ACCESSION   M97856
KEYWORDS    histone-binding protein.
SOURCE      Homo sapiens (library: Clontech) male 50 year old adult testis cDNA
            to mRNA.
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chorqata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (sites)
  AUTHORS   Kleinschmidt,J.A., Dingwall,C., Maier,G. and Franke,W.W.
  TITLE     Molecualr characterization of a karyophilic, histone-binding
            protein: cDNA cloning, amino acid sequence and expression of
            nuclear protein N1/N2 of Xenopus laevis
  JOURNAL   EMBO J. 5, 3547-3552 (1986)
  STANDARD  full automatic
REFERENCE   2  (sites)
  AUTHORS   Welch,J.E., Zimmerman,L.J., Joseph,D.R. and O'Rand,M.G.
  TITLE     Characterization of a nuclear autoantigenic sperm protein: Complete
            sequence and homology with the Xenopus protein, N1/N2
  JOURNAL   Biol. Reprod. 43, 559-568 (1990)
  STANDARD  full automatic
REFERENCE   3  (bases 1 to 2561)
  AUTHORS   O'Rand,M.G., Richardson,R.T., Zimmerman,L.J. and Widgren,E.E.
  TITLE     Sequence and localization of human NASP: Conservation of a Xenopus
            histone binding protein
  JOURNAL   Dev. Biol. 154, 37-44 (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     5'UTR           1..85
     3'UTR           2447..2561
                     /evidence="EXPERIMENTAL"
                     /citation=[3]
                     /citation=[2]
     polyA_signal    2537..2542
                     /citation=[3]
                     /citation=[2]
     CDS             86..2449
                     /function="binds histones"
                     /label=hbp
                     /citation=[1]
                     /citation=[3]
                     /citation=[2]
                     /note="putative histone binding domain I: aa 115-126;
                     putativehistone binding domain II: aa 469-511; nuclear
                     translocationsignal: aa 715-720; putative"
                     /product="histone-binding protein"
                     /codon_start=1
                     /translation="MAMESTATAAVAADVVSADKIEDVPAPSTSADKVESLDVDSEAK
                     KLLGLGQKHLVMGDIPAAVNAFQEAASLLGKKYGETANECGEAFFFYGKSLLELARME
                     NGVLGNALEGVHVEEEEGEKTEDESLVENNDNIDEEAREELREQVYDAMGEKEEAKKT
                     EDKSLAKPETDKEQDSEMEKGGREDMDISKSAEEPQEKVDLTLDWLTETSEEAKGGAA
                     PEGPNEAEVTSGKPEQEVPDAEEEKSVSGTDVQEECREKGGQEKQGEVIVSIEEKPKE
                     VSEEQPVVTLEKQGTAVEVEAESLDPTVKPVDVGGDEPEEKVVTSENEAGKAVLEQLV
                     GQEVPPAEESPEVQTEAAEASAVEAGSEVSEKPGQEAPVLPKDGAVNGPSVVGDQTPI
                     EPQTSIERLTETKDGSGLEEKVRAKLVPSQEETKLSVEESEAAGDGVDTKVAQGATEK
                     SPEDKVQIAANEETQEREEQMKEGEETEGSEEDDKENDKTEEMPNDSVLENKSLQENE
                     EEEIGNLELAWDMLDLAKIIFKRQETKEAQLYAAQAHLKLGEVSVESENYVQAVEEFQ
                     SCLNLQEQYLEAHDRLLAETHYQLGLAYGYNSQYDEAVAQFSKSIEVIENRMAVLNEQ
                     VKEAEGSSEYKKEIEELKELLPEIREKIEDAKESQRSGNVAELALKATLVESSTSGFT
                     PGGGGSSVSMIASRKPTDGASSSNCVTDISHLVRKKRKPEEESPRKDDAKKAKQEPEV
                     NGGSGDAVPSGNEVSENMEEEAENQLKRGAAVEGTLEAGATVESTAC"
BASE COUNT      841 a    446 c    757 g    517 t
ORIGIN      
        1 gcctgagtga gtctctggcg tcccaaattg cctgtttttc tcgcaggctc tattccgttc
       61 gctggttcgc cacctcaggg gaacgatggc catggagtcc acagccactg ccgccgtcgc
      121 cgcggacgtg gtttctgccg acaaaattga agatgtccct gctccttcta catctgcaga
      181 taaagtggag agtctggatg tggatagtga agctaagaaa ctattgggtt taggacagaa
      241 acatctggtg atgggggata ttccagcagc tgtcaatgca ttccaggaag cagctagtct
      301 tttaggtaag aagtatggag agacagctaa tgagtgtgga gaagccttct ttttctatgg
      361 gaaatcactt ctggagttgg caagaatgga gaatggtgtg ttgggaaacg ccttggaagg
      421 tgtgcatgtg gaagaggaag aaggagaaaa aacagaagat gaatctctgg tagaaaataa
      481 tgataacata gatgaggaag caagggaaga gttgagagaa caggtttatg acgccatggg
      541 agaaaaagaa gaagccaaaa aaacagaaga caagtctttg gcaaagcctg aaactgataa
      601 agaacaggac agtgaaatgg agaagggtgg aagagaagat atggatataa gtaaatctgc
      661 agaggagcca caggaaaaag ttgacttgac tctagattgg ttaactgaaa cctctgaaga
      721 ggcaaaagga ggagcagcac cagaaggacc gaatgaagct gaggtcactt ctgggaagcc
      781 agaacaggaa gtaccagatg ctgaggaaga aaaatcagtt tctggaactg atgtccaaga
      841 agagtgcaga gaaaaaggag gtcaggagaa gcagggagag gtaattgtga gcatagagga
      901 gaagccaaaa gaagtttcag aagagcagcc tgtggtgact ctagaaaagc agggcactgc
      961 agtggaggta gaagcagagt ctttagaccc gacagtcaag ccagtggatg tgggtgggga
     1021 cgagccagag gagaaggtag ttacctctga aaacgaggca ggaaaggcgg ttcttgaaca
     1081 actggtaggt caagaagtac cacctgctga agagtcacca gaggtgcaaa cagaggctgc
     1141 agaggcctca gctgtagagg ctggatcaga agtctctgaa aagcctgggc aggaggctcc
     1201 agttctccct aaggatggtg cagtcaatgg accgtcagtt gtaggagatc agactcctat
     1261 tgaaccacag acttctatag aaagactgac agaaacaaaa gatggctcag gactagagga
     1321 gaaggtcagg gcaaagctgg ttcctagtca ggaggagact aagctgtctg tagaagagtc
     1381 tgaggcagct ggagatgggg ttgataccaa ggtagcccag ggagctactg agaaatcacc
     1441 tgaagacaaa gttcagatag ctgctaatga agagacacaa gagagagaag aacagatgaa
     1501 agagggtgaa gaaactgaag gctcggaaga ggatgataaa gaaaatgata agactgaaga
     1561 aatgccaaat gattcagtcc ttgaaaacaa gtctcttcaa gaaaatgagg aggaggagat
     1621 tgggaaccta gagcttgcct gggatatgct ggatttagca aagatcattt ttaaaaggca
     1681 agaaacaaaa gaagcacagc tttatgctgc ccaggcacat cttaaactcg gagaagttag
     1741 tgttgaatct gaaaactatg tgcaagctgt ggaggagttc cagtcctgcc ttaacctgca
     1801 ggaacagtac ctggaagccc acgaccgtct gcttgcagag acccactacc agctgggctt
     1861 ggcttatggg tacaactctc agtatgatga ggcagtggca cagttcagca aatctattga
     1921 agtcattgag aacagaatgg ctgtactaaa cgagcaggtg aaggaggctg aaggatcgtc
     1981 tgaatacaag aaagaaattg aggaactaaa ggaactgcta cccgaaatta gagagaagat
     2041 agaagatgca aaggagtctc agcgtagtgg gaatgtagct gaactggctc tgaaagctac
     2101 tctggtggag agttctactt caggtttcac tcctggtgga ggaggctctt cagtctccat
     2161 gattgccagt agaaagccaa cagacggtgc ttcctcatca aattgtgtga ctgatatttc
     2221 ccaccttgtc agaaagaaga ggaaaccaga ggaagagagt ccccggaaag atgatgcaaa
     2281 gaaagccaaa caagagccgg aggtgaacgg aggcagtggg gatgctgtcc cgagtggaaa
     2341 tgaagtttcg gaaaacatgg aggaggaggc tgagaatcag ctgaaacgcg gagcagcagt
     2401 ggaggggaca ctggaggctg gagctacagt tgaaagcact gcatgttaag agggggcaca
     2461 gcctcctccc aagggaaagt gtttttgtat ataatgtatt ttttcacttt tggaggattc
     2521 tttttgtata acttcaataa agattgtaag caaaaaaaaa a
//