From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Mouse MHC class II H2-I-A-alpha gene (k haplotype), partial mRNA,
Date: 5 Jan 93 06:48:02 GMT
Distribution: bionet
Checksum: 54404 4

LOCUS       MUSMHIAAK     978 bp ss-mRNA            ROD       04-JAN-1993
DEFINITION  Mouse MHC class II H2-I-A-alpha gene (k haplotype), partial mRNA,
            complete coding sequence.
ACCESSION   V00832 J00399
KEYWORDS    antigen; cell surface glycoprotein; class II gene; glycoprotein;
            histocompatibility antigen; integral membrane protein;
            major histocompatibility complex.
SOURCE      Mouse (B10.AKM; k haplotype) spleen, cDNA to mRNA, clone pAAC6.
  ORGANISM  Mus musculus
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Rodentia; Myomorpha; Muridae; Murinae.
REFERENCE   1  (bases 1 to 30)
  AUTHORS   Benoist,C.O.
  JOURNAL   Unpublished (1983) Dept Medical Microbiology, Stanford U Med School
  STANDARD  full automatic
REFERENCE   2  (bases 31 to 978)
  AUTHORS   Benoist,C.O., Mathis,D.J., Kanter,M.R., Williams,V.E.II. and
            McDevitt,H.O.
  TITLE     The murine Ia alpha chains E-alpha and A-alpha show a surprising
            degree of sequence homology
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 80, 534-538 (1983)
  STANDARD  full automatic
COMMENT     Data kindly reviewed (05-MAY-1983) by C.O. Benoist. Compared in [2]
            with the E-alpha amino acid sequence. The two are about 50%
            homologous. [1] replaces the first 19 bp reported in [2].
FEATURES             Location/Qualifiers
     misc_feature    24..92
                     /note="This feature applies to a gene which lacks a coding
                     region feature. "
                     /gene="MHC H2-I-A-alpha chain"
     mRNA            <1..>978
                     /note="H2-I-A-alpha mRNA"
     sig_peptide     24..92
                     /gene="MHC H2-I-A-alpha chain"
                     /codon_start=1
     mat_peptide     93..791
                     /product="MHC H2-I-A-alpha chain"
                     /codon_start=1
     CDS             24..794
                     /note="precursor"
                     /product="MHC H2-I-A-alpha chain"
                     /codon_start=1
                     /translation="MPRSRALILGVLALTTMLSLCGGEDDIEADHVGSYGITVYQSPG
                     DIGQYTFEFDGDELFYVDLDKKETVWMLPEFAQLRRFEPQGGLQNIATGKHNLEILTK
                     RSNSTPATNEAPQATVFPKSPVLLGQPNTLICFVDNIFPPVINITWLRNSKSVTDGVY
                     ETSFFVNRDYSFHKLSYLTFIPSDDDIYDCKVEHWGLEEPVLKHWEPEIPAPMSELTE
                     TVVCALGLSVGLVGIVVGTIFIIQGLRSGGTSRHPGPL"
BASE COUNT      218 a    263 c    247 g    250 t
ORIGIN      44 bp upstream of HinfI site.
        1 gcagagacct cccggagacc aggatgccgc gcagcagagc tctgattctg ggggtcctcg
       61 ccctgaccac catgctcagc ctctgcggag gtgaagacga cattgaggcc gaccacgtag
      121 gctcctatgg tataactgta tatcagtctc ctggagacat tggccagtac acatttgaat
      181 ttgatggtga tgagttgttc tatgtggact tggataagaa ggagactgtc tggatgcttc
      241 ctgagtttgc tcaactgaga agatttgagc cccaaggtgg actgcaaaac atagctacag
      301 gaaaacacaa cttggaaatc ttgactaaga ggtcaaattc caccccagct accaatgagg
      361 ctcctcaagc gactgtgttc cccaagtctc ctgtgctgct gggtcagccc aacaccctta
      421 tctgctttgt ggacaacatc ttccctcctg tgatcaacat cacatggctc agaaatagca
      481 agtcagtcac agacggcgtt tatgaaacca gcttcttcgt caaccgtgac tattccttcc
      541 acaagctgtc ttatctcacc ttcatccctt ctgacgatga catttatgac tgcaaggtgg
      601 agcactgggg cctggaggag ccggttctga aacactggga acctgagatt ccagccccca
      661 tgtcagagct gacagagact gtggtgtgtg ccctggggtt gtctgtgggc cttgtgggca
      721 tcgtggtggg caccatcttc atcattcaag gcctgcgatc aggtggcacc tccagacacc
      781 cagggccttt atgagtcaca ccctggaaag gaaggcgtgt gtccctcttc atggaagaag
      841 tggtgtgctg ggtgacctgg cacagtgtgt tttctggacc aatttatggt gttctttttc
      901 ttcttcaagt gacccccaac ttgcttttcc cttggccctg aggctgtccc tctcacagct
      961 cacacaccct tggaattc
//