From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: E.coli acetohydroxy acid synthase (AHAS) I (ilvB) and acetolactate
Date: 29 Sep 92 10:03:01 GMT
Distribution: bionet
Checksum: 55584     8

LOCUS       ECOILVBPR    2470 bp ds-DNA             BCT       29-SEP-1992
DEFINITION  E.coli acetohydroxy acid synthase (AHAS) I (ilvB) and acetolactate
            synthase small subunit (ilvN) genes (from the ilv-leu operon),
            complete cds.
ACCESSION   J01633 X01131 X02541
KEYWORDS    acetohydroxy acid synthetase; attenuator; ilvB gene; ilvB operon;
            ilvN gene; leader peptide; promoter region; synthetase.
SOURCE      Escherichia coli DNA.
  ORGANISM  Escherichia coli
            Prokaryotae; Gram-negative facultatively anaerobic rods;
            Enterobacteriaceae.
REFERENCE   1  (bases 1 to 370)
  AUTHORS   Friden,P., Newman,T. and Freundlich,M.
  TITLE     Nucleotide sequence of the ilvB promoter-regulatory region: A
            biosynthetic operon controlled by attenuation and cyclic AMP
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 79, 6156-6160 (1982)
  STANDARD  full staff_review
REFERENCE   2  (bases 45 to 332)
  AUTHORS   Hauser,C.A. and Hatfield,G.W.
  TITLE     Nucleotide sequence of the ilvB multivalent attenuator region of
            Escherichia coli K12
  JOURNAL   Nucleic Acids Res. 11, 127-139 (1983)
  STANDARD  full staff_review
REFERENCE   3  (bases 1 to 370)
  AUTHORS   Friden,P., Tsui,P., Okamoto,K. and Freundlich,M.
  TITLE     Interaction of cyclic AMP receptor protein with the ilvB
            biosynthetic operon in E.coli
  JOURNAL   Nucleic Acids Res. 12, 8145-8160 (1984)
  STANDARD  full staff_review
REFERENCE   4  (bases 353 to 2436)
  AUTHORS   Friden,P., Donegan,J., Mullen,J., Tsui,P., Freundlich,M.,
            Eoyang,L., Weber,R. and Silverman,P.M.
  TITLE     The ilvB locus of Escherichia coli K-12 is an operon encoding both
            subunits of acetohydroxyacid synthase I
  JOURNAL   Nucleic Acids Res. 13, 3979-3993 (1985)
  STANDARD  full staff_review
REFERENCE   5  (bases 8 to 2470)
  AUTHORS   Wek,R.C., Hauser,C.A. and Hatfield,G.W.
  TITLE     The nucleotide sequence of the ilvBN operon of Escherichia coli:
            Sequence homologies of the acetohydroxy acid synthase isozymes
  JOURNAL   Nucleic Acids Res. 13, 3995-4010 (1985)
  STANDARD  full staff_review
COMMENT     The ilvB gene, coding for the isoenzyme acetohydroxy acid synthase
            I (AHAS I), is repressed by both valine and leucine, and it is also
            subject to catabolite repression. The potential leader polypeptide
            consists of 32 amino acids, 12 of which are valine and leucine.
            Transcription termination produces a leader mRNA of approximately
            188 nucleotides. [1],[2] offer two different models for the
            secondary structure of this leader and its role in attenuation of
            ilvB expression.
               EMBL features not translated to GenBank features
               key        from     to       description
               RBS         341    344       pot. ribosome binding site (ilvB)
               RBS        2031   2035       pot. ribosome binding site (ilvN)
FEATURES             Location/Qualifiers
     mRNA            114..>2041
                     /note="alternate"
                     /gene="ilvB"
     mRNA            118..>2041
                     /note="alternate"
                     /gene="ilvB"
     CDS             149..247
                     /note="ilv operon leader peptide"
                     /gene="ilvB"
                     /codon_start=1
                     /translation="MTTSMLNAKLLPTAPSAAVVVVRVVVVVGNAP"
     CDS             353..2041
                     /product="acetohydroxy acid synthase (AHAS) I"
                     /gene="ilvB"
                     /codon_start=1
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPGPVWIDIPKDVQTAVFEIETQPAMAEKAAAPAFSEESIRDAAAMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPLVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQASLQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     CDS             2045..2335
                     /gene="invN"
                     /codon_start=1
                     /translation="MQNTTHDNVILELTVRNHPGVMTDVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
BASE COUNT      574 a    670 c    675 g    551 t
ORIGIN      4 bp upstream from MspI site.
        1 gctccggcgg ggttttttgt tatctgcaat tcagtacaaa acgtgatcaa cccctcaatt
       61 ttccctttgc tgaaaaattt tccattgtct cccctgtaaa gctgtgcttg tataaatatt
      121 gttaaacaca aaaccaacaa ggtccccaat gactacttcc atgctcaacg caaaactact
      181 accaactgcg ccatccgccg cagtggtcgt cgtgcgtgtg gtggtggtcg tcggcaatgc
      241 gccgtaggga ctggaacaac acacgattcc aaaaccccgc cggcgcaaac cgggcggggt
      301 ttttcgttta agcaccgccc ggaaagtcgg cccagaagaa aaggactgga gcatggcaag
      361 ttcgggcaca acatcgacgc gtaagcgctt taccggcgca gaatttatcg ttcatttcct
      421 ggaacagcag ggcattaaga ttgtgacagg cattccgggc ggttctatcc tgcctgttta
      481 cgatgcctta agccaaagca cgcaaatccg ccatattctg gcccgtcatg aacagggcgc
      541 gggctttatc gctcagggaa tggcgcgcac cgacggtaaa ccggcggtct gtatggcctg
      601 tagcggaccg ggtgcgacta acctggtgac cgccattgcc gatgcgcggc tggactccat
      661 cccgctgatt tgcatcactg gtcaggttcc cgcctcgatg atcggcaccg acgccttcca
      721 ggaagtggac acctacggca tctctatccc catcaccaaa cacaactatc tggtcagaca
      781 tatcgaagaa ctcccgcagg tcatgagcga tgccttccgc attgcgcaat caggccgccc
      841 aggcccggtg tggatagaca ttcctaagga tgtgcaaacg gcagtttttg agattgaaac
      901 acagcccgct atggcagaaa aagccgccgc ccccgccttt agcgaagaaa gcattcgtga
      961 cgcagcggcg atgattaacg ctgccaaacg cccggtgctt tatctgggcg gcggtgtgat
     1021 caatgcgccc gcacgggtgc gtgaactggc ggagaaagcg caactgccta ccaccatgac
     1081 tttaatggcg ctgggcatgt tgccaaaagc gcatccgttg tcgctgggta tgctggggat
     1141 gcacggcgtg cgcagcacca actatatttt gcaggaggcg gatttgttga tagtgctcgg
     1201 tgcgcgtttt gatgaccggg cgattggcaa aaccgagcag ttctgtccga atgccaaaat
     1261 cattcatgtc gatatcgacc gtgcagagct gggtaaaatc aagcagccgc acgtggcgat
     1321 tcaggcggat gttgatgacg tgctggcgca gttgatcccg ctggtggaag cgcaaccgcg
     1381 tgcagagtgg caccagttgg tagcggattt gcagcgtgag tttccgtgtc caatcccgaa
     1441 agcgtgcgat ccgttaagcc attacggcct gatcaacgcc gttgccgcct gtgtcgatga
     1501 caatgcaatt atcaccaccg acgttggtca gcatcagatg tggaccgcgc aagcttatcc
     1561 gctcaatcgc ccacgccagt ggttgacctc cggtgggctg ggcacgatgg gttttggcct
     1621 gcctgcggcg attggcgctg cgctggcgaa cccggatcgc aaagtgttgt gtttctccgg
     1681 cgacggcagc ctgatgatga atattcagga gatggcgacc gccagtgaaa atcagctgga
     1741 tgtcaaaatc attctgatga acaacgaagc gctggggctg gtgcatcagc aacagagtct
     1801 gttctacgag caaggcgttt ttgccgccac ctatccgggc aaaatcaact ttatgcagat
     1861 tgccgccgga ttcggcctcg aaacctgtga tttgaataac gaagccgatc cgcaggcttc
     1921 attgcaggaa atcatcaatc gccctggccc ggcgctgatc catgtgcgca ttgatgccga
     1981 agaaaaagtt tacccgatgg tgccgccagg tgcggcgaat actgaaatgg tgggggaata
     2041 agccatgcaa aacacaactc atgacaacgt aattctggag ctcaccgttc gcaaccatcc
     2101 gggcgtaatg accgacgttt gtggcctttt tgcccgccgc gcttttaacg ttgaaggcat
     2161 tctttgtctg ccgattcagg acagcgacaa aagccatatc tggctactgg tcaatgacga
     2221 ccagcgtctg gagcagatga taagccaaat cgataagctg gaagatgtcg tgaaagtgca
     2281 gcgtaatcag tccgatccga cgatgtttaa caagatcgcg gtgttttttc agtaaccgct
     2341 caaggcttga acaacatcgc gcttatcgtt aaggtaagcg cgtatttttt ttacccgcca
     2401 ggacaagacc atgatcaccg ttgcccttat agacgatcac ctcatcgtcc gctccggctt
     2461 tgcgcagctg
//