From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: S.tuberosum mRNA for beta-isopropylmalate dehydrogenase
Date: 10 Sep 92 10:35:25 GMT
Distribution: bionet
Checksum: 47134     4

LOCUS       POTBISODEH   1406 bp ss-RNA             PLN       10-SEP-1992
DEFINITION  S.tuberosum mRNA for beta-isopropylmalate dehydrogenase
ACCESSION   X67310
KEYWORDS    3-isopropylmalate dehydrogenase;
            beta-isopropylmalate dehydrogenase; isopropylmalate dehydrogenase.
SOURCE      Solanum tuberosum RNA.
  ORGANISM  Solanum tuberosum
            Eukaryota; Plantae; Embryobionta; Magnoliophyta; Magnoliopsida;
            Asteridae; Solanales; Solanaceae.
REFERENCE   1  (bases 1 to 1406)
  AUTHORS   Jackson,S.D.
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
REFERENCE   2  (bases 53 to 1123)
  AUTHORS   Jackson,S., Sonnewald,U. and Willmitzer,L.
  TITLE     Cloning and expression analysis of beta-isopropylmalate
            dehydrogenase from potato
  JOURNAL   Mol. Gen. Genet. (1992) In press
  STANDARD  full automatic
COMMENT     *source: strain=cv. Desiree; *source: stage=mature; *source:
            tissue=leaf; *source: clone_library=lambda-ZAP II;
            From EMBL    entry TBBISODEH;  dated 28-JUL-1992.
FEATURES             Location/Qualifiers
     transit_peptide 53..139
                     /note="chloroplast transit peptide"
     CDS             53..1126
                     /EC_number="1.1.1.85"
                     /product="3-isopropylmalatedehydrogenase"
                     /codon_start=1
                     /translation="MALQIAKRLLRCRADSVASSVRFFDRTFTSESNSNLIRATLFPG
                     DGIGPEIAESVRQIFKVAEVPIEWEEHYVGTEVDPRTNSFLTWESLESVRRNKVGLKG
                     PMATPIGKGHRSLNLTLRKELNLYANVRPCYSLPGYKTRYDDVNLITIRENTEGEYSG
                     LEHQVVRGVVESLKIITRQASLRVAEYAFHYAKTHGRERVSAIHKANIMQKTDGLFLK
                     CCREVAEKYPEIKYEEVVIDNCCMMLVKNPALFDVLVMPNLYGDIISDLCAGLIGGLG
                     LTPSCNIGEGGIALAEAVHGSAPDIAGKNLANPTALLLSSVSMLRHLELHDKADRIQD
                     AILKTIAGGKVPNWRPWRHCYNN"
     polyA_signal    1251..1256
BASE COUNT      395 a    282 c    329 g    400 t
ORIGIN
        1 aattagtcca ctccctttac atcaacaatt tctgcaattc taaggatcta caatggctct
       61 tcagattgcg aaaagactcc ttcgatgccg cgctgattct gttgcatcct ctgttagatt
      121 tttcgatcgg accttcactt ccgaatccaa ttccaacttg atccgcgcta cgcttttccc
      181 cggcgatggt attggacctg aaatcgccga gtccgtcagg cagatattca aggttgctga
      241 ggtaccaatt gaatgggaag aacactatgt ggggacggag gtagacccta gaaccaacag
      301 ctttctaaca tgggaaagtc ttgaatcagt gagacggaat aaggttggtt tgaaagggcc
      361 aatggccact cctattggaa aaggtcatcg ttccttgaac cttacattga ggaaggagtt
      421 aaatctatat gccaatgtca gaccttgcta cagcctacct ggatataaga ctcgctacga
      481 tgatgtgaat ctcatcacta ttagagagaa cactgaagga gagtacagtg gtcttgaaca
      541 tcaagtagta agaggtgtgg ttgaaagtct caagatcatt acccgtcaag caagtttaag
      601 ggttgcagag tatgcatttc actatgccaa gacccatgga agagagagag tgtctgcaat
      661 tcacaaagcc aacatcatgc agaagacaga tggtcttttt cttaagtgtt gccgagaggt
      721 cgcggaaaag taccctgaga taaagtatga ggaagttgtc attgacaatt gctgtatgat
      781 gcttgtgaaa aatccagcac tttttgatgt tctggtgatg cctaatcttt atggtgacat
      841 aatcagtgat ctttgtgctg gtttgattgg tggtttgggc ttaactccaa gttgcaatat
      901 tggcgaggga ggtattgccc ttgctgaggc tgtgcatggt tcagcacctg atattgccgg
      961 aaagaatttg gcgaatccaa ctgctttgct tttgagttct gtctctatgc ttcgtcatct
     1021 agagcttcat gataaagctg atcgcatcca agatgccatc ctaaaaacaa ttgcaggagg
     1081 gaaagtaccg aactggcgac cttggaggca ctgctacaac aactgagttc acaaatgcca
     1141 tctgcgatca tctttgaaca tgtgtatata tatatatagt caattttgat agttggaggt
     1201 gaccttggag cctatggcga acccaggatt tgaagacttg ggggtgaacc aataaactta
     1261 aaataaaaac aatgaaaata ttgggatctc tagctagatt tgaaccctga tctcatgggt
     1321 ggtacttcta gcttgtacca acggcacaag agcactccta tattcctcac gaaggtgtca
     1381 ttgctaatta tattctagtt tttggc
//