From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: D.pseudoobscura Adh gene for alcohol dehydrogenase
Date: 28 Dec 92 21:27:23 GMT
Distribution: bionet
Checksum: 42484 8

LOCUS       DROADHGN48   3464 bp ds-DNA             INV       01-DEC-1992
DEFINITION  D.pseudoobscura Adh gene for alcohol dehydrogenase
ACCESSION   X62228
KEYWORDS    ADH gene; alcohol dehydrogenase.
SOURCE      Drosophila pseudoobscura DNA.
  ORGANISM  Drosophila pseudoobscura
            Eukaryota; Animalia; Metazoa; Arthropoda; Uniramia; Insecta;
            Pterygota; Neoptera; Holometabola; Diptera; Brachycera;
            Cyclorrhapha; Schizophora; Drosophiloidea; Drosophilidae.
REFERENCE   1  (bases 1 to 3464)
  AUTHORS   Schaeffer,S.W. and Miller,E.L.
  TITLE     Estimates of gene flow in Drosophila pseudoobscura determined from
            nucleotide sequence analysis of the alcohol dehydrogenase region
  JOURNAL   Genetics 132, 471-480 (1992)
  STANDARD  full automatic
COMMENT     *source: strain=OREG3; *source: isolate=Brookings, Oregon; *source:
            chromosome=IV section 88;
               From EMBL    entry DPADHGN48;  dated 16-OCT-1992.
FEATURES             Location/Qualifiers
     mRNA            join(40..119,920..1025,1089..1493,1554..1969)
                     /note="adult mRNA (Adh)"
     mRNA            join(887..1025,1089..1493,1554..1969)
                     /note="larval mRNA (Adh)"
     promoter        8..14
                     /note="adult promoter (Adh)"
     prim_transcript 40..1969
                     /note="adult primary transcript (Adh)"
     exon            40..119
                     /note="adult exon 1a"
     intron          120..919
                     /note="adult intron"
     promoter        851..858
                     /note="larval promoter (Adh)"
     prim_transcript 887..1969
                     /note="larval primary transcript (Adh)"
     exon            887..1025
                     /note="larval exon 1"
     exon            920..1025
                     /note="adult exon 1b"
     intron          1026..1088
                     /number=1
     exon            1089..1493
                     /number=2
     intron          1494..1553
                     /number=2
     exon            1554..1969
                     /number=3
     polyA_signal    1946..1951
     polyA_site      1969
     CDS             join(933..1025,1089..1493,1554..1820)
                     /EC_number="1.1.1.1"
                     /gene="Adh"
                     /product="alcoholdehydrogenase"
                     /codon_start=1
                     /translation="MSLTNKNVVFVAGLGGIGLDTSRELVKRNLKNLVILDRIDNPAA
                     IAELKAINPKVTITFYPYDVTVPVAETTKLLKTIFAQVKTIDVLINGAGILDDHQIER
                     TIAVNYTGLVNTTTAILDFWDKRKGGPGGIICNIGSVTGFNAIYQVPVYSGSKAAVVN
                     FTSSLAKLAPITGVTAYTVNPGITKTTLVHKFNSWLDVEPRVAEKLLEHPTQTSQQCA
                     ENFVKAIELNKNGAIWKLDLGTLEPITWTQHWDSGI"
BASE COUNT      969 a    795 c    793 g    907 t
ORIGIN      
        1 cgtagtgtat ttaaggcact gcacatgtcg aggaccacga ttattgtcta cagtgcagtc
       61 agtagttgtc aggtgcagca ggcggaccag catttcctta aatctactca atcgatcaag
      121 taagtaactg taatccgagt acagtcggcc catcccactc ggttctgcag taactcaaga
      181 aatttcttga tagcgcagtt ccagttgtaa aacgtcgttt aaaaaaacat ctcgttggag
      241 cttgggttgg aaaacgttcg agttgggcgc agaaaatttt aaatataaaa taacgcaaaa
      301 gagaaaatat cttcccaaag cagctgtcca ttttttgtta aagtgataga tcaaaaattc
      361 gtatgacgta tttcccaagt gctctttgtg caaataattt cctcaattct aattctacaa
      421 gaagcacttt tggctcttgt ccagaaaaaa caatttgttt acacttgtcg gttgataaca
      481 gaggcggacc gacggctaag taacataaca gtcgcctgtt aagtaatgcc ctcactctct
      541 gttagatggc cgcgcgtgtc gactccgata gaccagcaga cacgcagtag ttttcctcaa
      601 gaacctgaac tctaaacata gacataattt acgatacctt gagactcaaa taaaaataca
      661 agtatgagtg gtattctatt cctgattaga ctcgcacaca cacacgtacg agtattaaca
      721 taaatttgcc ggcaaataac caaaacaaaa cgaacgcaaa cgtaccgatc cgaacacgta
      781 atgcgagaga taagaaacag aaaagctcga cgtgaaagaa gcttctggcg gaagctcttg
      841 ccgaactccc tataaatacg agacagaaaa ccagaataaa tataataaca aactgttgaa
      901 ttgtcccatc gattcccaga tcaaaagaca gaatgtctct caccaacaag aacgtcgttt
      961 tcgtggccgg tctgggcggc attggcctgg acaccagccg ggagttggtc aagcgtaatc
     1021 tgaaggtaag agagaacgaa ttccatggaa tctatggaat cctaaattta aaaattcatt
     1081 tattttagaa cctggtcatc ctggatcgca ttgacaatcc ggctgccatt gccgaactga
     1141 aggcaatcaa tcccaaggtg accatcacct tctatcccta cgatgtgact gtgcccgtcg
     1201 ctgagaccac caagctcctg aagaccatct ttgcccaggt gaagacaatc gatgtcctga
     1261 tcaacggtgc tggcatcctg gacgatcatc agatcgagcg caccattgcc gttaactaca
     1321 cgggcctggt caacaccacc acagccattc tggacttctg ggacaagcgc aagggcggcc
     1381 caggcggcat catttgcaac attggctccg tcaccggttt caatgccatc taccaggtgc
     1441 ccgtttactc cggctccaag gcggcggtgg ttaactttac ctcctccctg gcggtaagta
     1501 atccaacgtt tttcctactc cccaaaacta aatatttctt tgaaatccca tagaaactgg
     1561 ctcccattac tggtgtcact gcttacactg tcaatcctgg catcactaag accactctgg
     1621 tccacaaatt caactcgtgg ctggatgtgg agccccgtgt ggcggagaag ctgctcgagc
     1681 atcccaccca gacctctcag cagtgcgccg agaactttgt gaaggccatc gagctgaaca
     1741 aaaacggtgc catctggaag ttggatttgg gcaccttgga gcccatcaca tggacccagc
     1801 actgggactc gggcatctaa accatcccag agactctatg ggacatggcg ttttagcttt
     1861 tagtttcttt ttttccactc aattgttacg tatatacata cacatatggc aataaggctg
     1921 atttgaaccc cgttttgaat atgataatac aaaattatat ttgagaaatt tcaacaaaat
     1981 cgataagagc tatagctctc ttttaattaa aaaaaactag ataaaaagag caatggtcag
     2041 tggtcattgt ggcctctatc gatttccaca caaaaacttt actgtttagt aatagaaaag
     2101 aacttgagaa aggcagccaa aagaatgtac gatctgacgg gtaagcatgt ctgctatgtg
     2161 gctgactgcg gtggcattgc actggagacc agcaaggttc tcatgaccaa gaatatagcg
     2221 gtgagtggta gagaggtggt gtagagagtg gagagtgatt ctggttctta aacaagtctc
     2281 tagtctctag tctcttggct gctgctggta gctgtcgaaa ctaattaagt taatgacttt
     2341 ttcatgtttt gtttttgtcc gctgactttt gctgttcaaa gctacaatta agtttatgtt
     2401 ttgagtttga tctacttttg cttaagggaa gtactttcga ttacggattg ctggcatata
     2461 aaaatagata ccaaggaatg tgcatcatat catattgatt ccatcttgat tctttgccca
     2521 gaaactggcc attcttcaga gcgtggaaaa ccccccggcc atcgctcagt tacaatcgat
     2581 taagcacagc acacagatct tcttctggac cttcgatgtg accatggccc gcgaggagat
     2641 gaagaagtac ttcgatgagg tcatggtcca gatggactac atcgatgtgc tgatcaatgg
     2701 ggccaccctg tgcgatgagc ggaacattga tgccaccatc aatacgaatc tgaccggcat
     2761 gatgaacacc gtggccactg tgctgcccta tatggaccga aagatgggcg gttcgggtgg
     2821 actgatcgtg aatgtcacct ccgtcatagg actggatcca tcgccagtct tttgtgcgta
     2881 cagtgcctca aagtttggtg tgattgggtt cacccgaagt ctagcggtga gtgggcggga
     2941 tcttaacttg aaaatgctct ctaatgtctt ccattctttc gcaggatccc ctgtattaca
     3001 cccaaaatgg tgtggctgtc atggccgtct gctgtggtcc caccaaagtg tttgtcgatc
     3061 gggaactgac tgccttcctg gcatacggcc aaacctttgc cgatcgcctg cgtcgtgccc
     3121 cctgccaatc gacggccgtc tgcggccaga atatagttaa tgccatcgag agatcggaga
     3181 acggacagat ctggattgcc gacaagggcg gcctggagtc ggtggccctc cactggtatt
     3241 ggcatatggc cgatcagttt gtgaactata tgcagagcac tgacgatgag gatcaggagt
     3301 tttttttggg ccagcgatga ggcatgcctt gatccacgga ataaaacatc aaatgtgtca
     3361 caaaaagtag gcaacttgtt tgtgggtctg gaaatagatt tgaaatattt tgaggtcata
     3421 tgaataatga cataaatgag ggtgcctgaa agtatgccaa gttt
//