From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Klebsiella oxytoca tnpR gene, 3' end and TEM-12 beta-lactamase
Date: 24 Jul 92 10:07:50 GMT
Distribution: bionet
Checksum: 43697     6

LOCUS       KPNBETALAC   1268 bp ds-DNA             BCT       24-JUL-1992
DEFINITION  Klebsiella oxytoca tnpR gene, 3' end and TEM-12 beta-lactamase
            (blaT-12) gene, complete cds.
ACCESSION   M88143
KEYWORDS    TEM-12 beta-lactamase; extended-spectrum beta-lactamase.
SOURCE      Klebsiella oxytoca (strain 0400-1) DNA.
  ORGANISM  Klebsiella oxytoca
            Prokaryotae; Gram-negative facultatively anaerobic rods;
            Enterobacteriaceae.
REFERENCE   1  (bases 1 to 1268)
  AUTHORS   Heritage,J., Hawkey,P.M., Todd,N. and Lewis,I.J.
  TITLE     Transposition of the gene encoding the TEM-12 extended-spectrum
            beta-lactamase produced by an isolate of Klebsiella oxytoca from a
            blood culture of a child with leukaemia
  JOURNAL   Unpublished (1992)
  STANDARD  full staff_entry
COMMENT     The ultimate source of this sequence was strain 0400-1,
            an isolate of Klebsiella oxytoca which is resistant to the
            antibiotic ceftazidime. The resistance gene is located on a
            transposon Tn841, which is found on a self-transmissible plasmid.
            I introduced this plasmid into a derivative of E.coli strain UB5201
            which was carrying the multicopy plasmid pACYC184. I then obtained
            a recombinant plasmid in which the ceftazidime resistance
            determinant located on Tn841 had inserted itself, and the rest of
            Tn841 into plasmid pACYC184, giving the recombinant plasmid
            pACYC184::Tn841, and it was from this recombinant plasmid that
            I derived my sequence.
FEATURES             Location/Qualifiers
     CDS             1..185
                     /gene="tnpR"
                     /codon_start=3
                     /translation="TNEGRQEAKLKGIKFGRRRTVDRNVVLTLHQKGTGATEIAHQLS
                     IARSTVYKILEDERAS"
                     /partial
     mutation        321
                     /note="'t' in Tn841 vs. 'g' in Tn3 at nucleotide 3903"
                     /note="silent mutation varying from Tn3 sequence"
     CDS             368..1228
                     /note="At nucleotide 851 the codon reads SER (amino acid
                     162) in Tn841 and that at nucleotide 4443 in Tn3 the codon
                     reads ARG (amino acid 162), and it is this change which is
                     probably responsible for the inc"
                     /product="TEM-12 beta-lactamase"
                     /gene="blaT-12"
                     /codon_start=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DSWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     mutation        505
                     /note="'g' in Tn841 vs. 'a' in Tn3 at nucleotide 4087"
                     /note="silent mutation varying from Tn3 sequence"
     mutation        595
                     /note="'t' in Tn841 vs. 'c' in Tn3 at nucleotide 4177"
                     /note="silent mutation varying from Tn3 sequence"
     mutation        841
                     /note="'c' in Tn841 vs. 't' in Tn3 at nucleotide 4423"
                     /note="silent mutation varying from Tn3 sequence"
     mutation        851
                     /note="'a' in Tn841 vs. 'c' in Tn3 at nucleotide 4433"
                     /note="mutation increasing spectrum of activity"
     mutation        1084
                     /note="'a' in Tn841 vs. 'g' in Tn3 at nucleotide 4666"
                     /note="silent mutation varying from Tn3 sequence"
     misc_feature    1247..1268
                     /note="divergence from Tn3 sequence"
BASE COUNT      345 a    282 c    319 g    322 t
ORIGIN
        1 gcacgaatga gggccgacag gaagcaaagc tgaaaggaat caaatttggc cgcaggcgta
       61 ccgtggacag gaacgtcgtg ctgacgcttc atcagaaggg cactggtgca acggaaattg
      121 ctcatcagct cagtattgcc cgctccacgg tttataaaat tcttgaagac gaaagggcct
      181 cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg
      241 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
      301 aaatatgtat ccgctcatga tacaataacc ctgataaatg cttcaataat attgaaaaag
      361 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
      421 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
      481 gggtgcacga gtgggttaca tcgagctgga tctcaacagc ggtaagatcc ttgagagttt
      541 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggtgcggt
      601 attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
      661 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
      721 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
      781 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
      841 ccgccttgat agttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
      901 cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
      961 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     1021 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     1081 tggatctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     1141 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     1201 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttacggac tgttgcaaag
     1261 ttagcgat
//