From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Giardia lamblia (clone pMM59, 3.5 kb SalI fragment subcloned in
Date: 29 Dec 92 01:17:12 GMT
Distribution: bionet
Checksum: 43928 4

LOCUS       GIATRIISO    1583 bp ds-DNA             INV       07-DEC-1992
DEFINITION  Giardia lamblia (clone pMM59, 3.5 kb SalI fragment subcloned in
            pGEM-4) triosephosphate isomerase gene, complete cds.
ACCESSION   L02120
KEYWORDS    triosephosphate isomerase.
SOURCE      Giardia lamblia (individual_isolate WB) (library: WB/1267 Sau3A
            partial genomic in lambda-FixII) trophozoite DNA.
  ORGANISM  Giardia lamblia
            Eukaryota; Animalia; Protozoa; Sarcomastigophora; Mastigophora;
            Zoomastigophora; Diplomonadida; Diplomonadina; Hexamitidae.
REFERENCE   1  (bases 1 to 1583)
  AUTHORS   Mowatt,M.R., Howard,T.C., Nash,T.E. and Weinbach,E.C.
  TITLE     Complementation of an Escherichia coli glycolysis mutant by Giardia
            lamblia triosephosphate isomerase
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     5'UTR           519..527
                     /citation=[1]
                     /note="determined by primer extension sequence analysis"
     polyA_signal    1357..1363
                     /note="putative"
     CDS             528..1301
                     /EC_number="5.3.1.1"
                     /product="triosephosphate isomerase"
                     /codon_start=1
                     /translation="MPARRPFIGGNFKCNGSLDFIKSHVAAIAAHKIPDSVDVVIAPS
                     AVHLSTAIAANTSKQLRIAAQNVYLEGNGAWTGETSVEMLQDMGLKHVIVGHSERRRI
                     MGETDEQSAKKAKRALEKGMTVIFCVGETLDERKANRTMEVNIAQLEALGKELGESKM
                     LWKEVVIAYEPVWSIGTGVVATPEQAEEVHVGLRKWFAEKVCAEGAQHIRIIYGGSAN
                     GSNCEKLGQCPNIDGFLVGGASLKPEFMTMIDILTKTRT"
BASE COUNT      386 a    384 c    432 g    381 t
ORIGIN      
        1 ggcgcattcc atggatggag gttcctgtcc cgggggttaa gcgatttctt gaggaggcta
       61 tcgaggtcct tcattctcac atagtagatg ataacacatt agtgcgagtc tcgggccttt
      121 cggagcgtcc ctttgggcgg ttatcctatg acactgttat atctaaaaga atatatgcgc
      181 tagtgctatc taatgacgtg ctacactcca tagatcttca tacttgtatc agcgaatgta
      241 gttctgtggt gtttcttcta ttctgcttca tgcaccgtga tttggacccc gacaagcttc
      301 tggcacaata cagtaatgtt attatatttg atattcttcg acctgactat gaccaactcc
      361 agcacgtact tctttccatc tgcagttgca ttcgctacaa tgctgaaagc gaaaaacgca
      421 atcactgtat ctgtggatat cacattggct aaagggttat gccctggaca ccattggctg
      481 cctcggatca agacttcaaa ttagaaattc tagccagaaa ataaatcatg cctgctcgtc
      541 gccccttcat cggcggtaac ttcaagtgta acggctctct tgactttatc aagagccacg
      601 tggcggcaat tgctgcccat aagatccctg attccgtgga cgtcgtcatt gccccttccg
      661 ccgtacacct gtcaacagcc attgcggcaa acacgtcaaa acagttgagg atagcagcgc
      721 agaatgtgta cctagagggg aacggggcgt ggactggcga gacaagtgtt gagatgcttc
      781 aggacatggg tttgaagcat gtgatagtag ggcactctga aagacgcaga atcatggggg
      841 agaccgacga gcaaagcgcc aagaaggcta agcgtgccct ggaaaagggg atgacggtca
      901 tcttctgcgt cggagagacc ttggacgagc gcaaggccaa ccgcaccatg gaggtgaaca
      961 tcgcccagct tgaggcgctt ggcaaggagc tcggagagtc caagatgctc tggaaggagg
     1021 ttgtcattgc ttacgagccc gtgtggtcca ttggcacggg cgtggtggcc acgcccgagc
     1081 aggcagagga ggtccatgtg gggctccgaa agtggtttgc ggagaaggtt tgtgccgagg
     1141 gcgcacagca tatccgtatc atttacgggg gatcggccaa tggaagcaac tgcgagaagc
     1201 ttggccagtg tccgaatatt gacggcttcc ttgtcggtgg cgcttccctc aagccggagt
     1261 ttatgacgat gatcgacatt cttacgaaga cccgtacata ggcatgtggc taagcgtgtt
     1321 gtaagctctt gacctctggg ctgtatcaca tccaagagta aattgacaaa taaaggcgct
     1381 agcaaataaa tggtgctggc cgttctggct cttgccctgc ttttcgcaag caccgcagag
     1441 gcccagaatc tggcccttga cgttatctca gtctccgata cacatgggtg gatttatggc
     1501 cacagacacc agaaggagct aggagactac ggaacccttg tgtcttacat cgaacgcacc
     1561 agggacgagg cagccaagga tcc
//