From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Clostridium pasteurianum isopropylmalate isomerase (leuD) gene 3'
Date: 21 Dec 92 20:40:38 GMT
Distribution: bionet
Checksum: 44711 6

LOCUS       CLOLEUILV    2327 bp ds-DNA             BCT       20-NOV-1992
DEFINITION  Clostridium pasteurianum isopropylmalate isomerase (leuD) gene 3'
            end; isopropylmalate dehydrogenase (leuB) gene complete cds;
            dehydroxyacid dehydrogenase (ilvD) gene, 5' end.
ACCESSION   L06666
KEYWORDS    dehydroxyacid dehydrogenase; ilvD gene;
            isopropylmalate dehydrogenase; isopropylmalate isomerase;
            leuB gene; leuD gene.
SOURCE      Clostridium pasteurianum (library: ATCC 6013) DNA.
  ORGANISM  Clostridium pasteurianum
            Prokaryotae; Firmicutes; Endospore-forming rods and cocci;
            Bacillaceae.
REFERENCE   1  (bases 1 to 2327)
  AUTHORS   Oultram,J.D., Loughlin,M., Walmsley,R., Gunnery,S.M. and
            Minton,N.P.
  TITLE     The nucleotide sequence of genes involved in the leucine
            biosynthetic pathway of Clostridium pasteurianum ATCC 6013
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     CDS             <2..304
                     /function="unknown"
                     /gene="leuD"
                     /note="putative"
                     /product="isopropylmalate isomerase"
                     /codon_start=1
                     /translation="HAPIALKAAGVSCVIAKSFARIFYRNSINIGFPILECEEAVNDA
                     KNNHELEVDFTTGIIKNITLGKEYKAQAYPQFMIDIMKNEGLINCVKNGALNSFMR"
     CDS             382..1455
                     /gene="leuB"
                     /evidence="EXPERIMENTAL"
                     /citation=[1]
                     /product="isopropylmalate dehydrogenase"
                     /codon_start=1
                     /translation="MKEFKIAVIPGDGIGPDIVREAVKIMTKVGEKYDTKFNFVEVKA
                     GGDAIDAYGEPLPKETIDVCKSSAAVLLGAVGGPKWDNLEGSKRPERALLGLRGALGL
                     YANLRPAKVYNVLKSASPLKNEIIDEGVDLLVVRELIGGIYFGDRGTKEVNGVETAFD
                     TEKYNVDEVKRIAHSAFKAAMKRRKKVTSVDKANVLDASRLWRKTVNEVSKEYPEVEL
                     SHLYVDNTAMQLVRKPSQFDVILTNNIFGDILSDEASMITGSIGMLASSSIREDSFGM
                     YEPIHGSAPDIAGLDIANPLAQILSAAMLMEYSLDMSEAARDVEAAVEKVLNEGYRTA
                     DIYIEGTKKVGTSEMGNLVLERL"
     CDS             1470..>2327
                     /gene="ilvD"
                     /note="putative"
                     /citation=[1]
                     /product="dihydroxyacid dehydrase"
                     /codon_start=1
                     /translation="MRSDSARKGLEKAPHRSLFKALGFIDEEMERPLIGIASSYSEII
                     PGHMNLDKVVQAVKDGVRMAGGVPVTFGTIGVCDGISMNHKGMSYSLPSRQLIADSVE
                     IVASAHAFDGIVLVPNCDKVVPGMMMAAARLDIPAIVISGGPMLAGNTCGKATDLSGV
                     FEAVGAVKAGNMTEEELSELENTACPTCGSCSGMYTANSMNCLSEVLGLALPYNGTIP
                     AVYSERLRLAKTSGMKIVDLVKKGLTPSKILTEEAFMNAITADMALGCSTNSLLHLPA
                     IANEVGITID"
BASE COUNT      833 a    311 c    506 g    677 t
ORIGIN      
        1 gcatgctcct atagcattaa aagcagcagg agtatcctgt gttattgcta aaagctttgc
       61 aagaatattc tatagaaatt ccataaatat aggattccca atacttgaat gtgaagaggc
      121 tgtaaatgat gcaaaaaata atcatgaact tgaagttgat tttactacag gtataataaa
      181 aaatataact ttgggaaaag aatataaagc acaggcttat cctcagttta tgatagatat
      241 tatgaaaaat gaaggactta taaactgtgt taaaaatgga gctttaaata gttttatgag
      301 gtaatatata gtcaacggat aaaaattttg ttacatttta gatacgtata taaatatgga
      361 ttataaggag cttgaatata gatgaaagaa tttaaaattg cagttatacc tggagacggt
      421 ataggtccgg atattgtaag agaagctgta aagataatga ctaaagttgg agaaaaatat
      481 gatacaaaat tcaattttgt agaagttaaa gctgggggag atgccataga tgcttatggt
      541 gaaccacttc ctaaggaaac aatagatgtg tgcaaaagca gtgcggctgt acttttagga
      601 gcagtgggag gacctaagtg ggataatctt gaaggctcta aaagaccaga gagagccctt
      661 ctaggacttc gtggtgccct tggcttatat gcaaatctaa gaccagcaaa agtttataat
      721 gtattaaaat ctgcatctcc tttaaaaaat gaaataatag atgagggcgt tgatctatta
      781 gtagtaagag aacttatagg tggtatatat tttggagata gaggaactaa agaggtaaac
      841 ggagtagaaa cagcttttga cacagaaaaa tataatgtag atgaagttaa aagaatagct
      901 cattctgctt ttaaagctgc aatgaagaga agaaagaaag ttacttcagt ggataaagcc
      961 aatgtactgg atgcatcaag attatggaga aaaactgtaa acgaagtatc taaagaatat
     1021 cctgaagtag aattaagtca tctatacgtt gataatactg ctatgcagct ggttagaaag
     1081 ccatctcaat ttgatgtaat attaacaaac aatatatttg gtgatatatt atcagatgag
     1141 gcttctatga taacaggttc tataggaatg cttgcatctt caagtataag agaggacagc
     1201 tttggaatgt atgaacctat ccatggttca gctcctgata tagctggact ggatatagct
     1261 aacccccttg cacagatact ttcagctgct atgcttatgg agtattctct tgatatgtca
     1321 gaagcagcaa gagatgtaga agcagcagta gaaaaagtat taaatgaagg atatagaact
     1381 gcagatatat atattgaagg gactaaaaaa gttggaacat cagaaatggg aaatttagtt
     1441 cttgaacgac tatagaggag ggattttata tgagatcgga ttcagctaga aagggtttag
     1501 aaaaagcccc acatagatca ctttttaaag ccttaggttt tatagatgaa gaaatggaaa
     1561 gaccacttat tggtattgca agttcctata gtgaaataat accaggtcat atgaatttag
     1621 ataaagttgt acaagcggta aaagatggag taagaatggc tggaggagtt ccagtaactt
     1681 ttggaactat aggtgtttgt gatggtatat ccatgaatca taagggtatg agttattcat
     1741 taccttcaag acagttaatt gcagatagcg tagaaatagt tgcaagtgcc catgcctttg
     1801 atggtatagt acttgtacct aattgtgata aggttgttcc aggaatgatg atggcggcag
     1861 cgagacttga tattcctgca atagttataa gtggtgggcc aatgcttgca ggtaatactt
     1921 gtggtaaagc tacagactta agcggagtat ttgaagcagt aggagctgta aaagcaggaa
     1981 atatgactga agaggaactt tcagaacttg aaaatacagc ttgtcctaca tgtggctcct
     2041 gttcaggtat gtatacagct aattctatga attgtctttc agaagttctt ggacttgccc
     2101 ttccttataa tggaacaata ccagcagtat actctgaaag attaagattg gctaagactt
     2161 ctggaatgaa aatagtagac cttgtaaaaa aaggtttaac accatcaaaa atacttactg
     2221 aagaagcttt tatgaatgct ataacagcag atatggctct tggatgttct acaaactctc
     2281 tacttcattt accagctata gctaatgaag ttggaataac tatcgat
//