From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Bacillus stearothermophilus DNA modification methyltransferase
Date: 29 Dec 92 19:49:09 GMT
Distribution: bionet
Checksum: 58711 5

LOCUS       BACBSTVIM    1900 bp ds-DNA             BCT       09-DEC-1992
DEFINITION  Bacillus stearothermophilus DNA modification methyltransferase
            (VIM) gene, complete cds.
ACCESSION   L07642
KEYWORDS    methyltransferase; restriction-modification system.
SOURCE      Bacillus stearothermophilus (strain ) DNA.
  ORGANISM  Bacillus stearothermophilus
            Prokaryotae; Firmicutes; Endospore-forming rods and cocci;
            Bacillaceae.
REFERENCE   1  (bases 1 to 1900)
  AUTHORS   Vasquez,C., Saavedra,C. and Gonzalez,E.
  TITLE     Cloning the BstVI restriction-modification system in Escherichia
            coli
  JOURNAL   Gene 102, 83-85 (1991)
  STANDARD  full automatic
REFERENCE   2  (bases 1 to 1900)
  AUTHORS   Gonzalez,E., Saavedra,C. and Vasquez,C.
  TITLE     Sequence of the restriction-modification system from Bacillus
            stearothermophilus V
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     -35_signal      113..118
     -10_signal      141..147
     RBS             191..193
                     /note="putative"
     CDS             202..1887
                     /function="methylates the adenine residue in the sequence
                     5'-CTCGAG-3'"
                     /standard_name="BstVI methylase"
                     /gene="VIM"
                     /evidence="EXPERIMENTAL"
                     /citation=[1]
                     /product="DNA modification methyltransferase"
                     /codon_start=1
                     /translation="MVTQHSYRMHGAVYTKPIIVDLILDLTGYTSDKNLENFKLLDPS
                     FGDGVFLEAAVHRLMDSLIRRGYRPNELIDHLGNCIRGIELRLEAYQAGRHRLQKVLE
                     GYGFSKPEINWLINQWIIQADFLLWQEDTTEAIKFDFVVGNPPYVRQELIQDELIKKY
                     RKRYTTIYDRADLYVPFIQHSLELLSEQGTLGIICSDRFTKNRYGKKLRKFITDNYKV
                     RYIVDLHKTSPFENEVTAYPAIYVIKTKNYDKSVVRAVYTEVITSKVCQDAKDFLLSN
                     QKPDQSSKEMKTYVFSEWFAGDEPWIIQSQECREILRRLENRFPLIEDDVHSCKIRIG
                     VATGADKVYIVDPQQVDIEPEVLLPLVTTADISSGRIIWSGKHVINPFNSDGGLINLD
                     DFPRLKTYFQQHEEIIKNRNVAKKNPSQWFRTIDRIYPEIVHQPKLLIPDMKNTNHIV
                     KDDGAFYPHHNLYYILPGNWNIDILRAILLSSVVKFFIWSYATKMRGDTLRYQAQYLR
                     KIRLPDPKSLTNDQKERLMDERVIQSQEYLDSIVAEIYQLSKTEIEIIKDALE"
BASE COUNT      621 a    335 c    380 g    564 t
ORIGIN      
        1 aagcttccat acattcaaac gggcagttca taaccttatc ttttctctgc ttttcattaa
       61 gagcagagtg tatgaccata acataattat cgctatccat tacgtagtat atgtgccagg
      121 gggaagggaa cttaaaacca tatttaagaa gaaatatgtt attcttagga atagacctat
      181 atgtagtggg ggtaactcta tatggtaaca caacatagtt atagaatgca tggagcggtc
      241 tacaccaaac ccataattgt tgaccttata ctagatttaa cagggtatac ttcggacaaa
      301 aatcttgaga actttaaact cttagacccg tcatttggag atggagtatt cctagaagca
      361 gccgtccatc gtctgatgga ctcgttgata agacgtggat atcgtcctaa cgaattaata
      421 gatcacttag gtaattgtat tcgagggata gagttaaggt tagaagccta ccaagctggt
      481 cgacatcggt tacaaaaagt gttagaggga tatggttttt ccaagccgga gattaactgg
      541 ttaatcaatc aatggatcat ccaagctgat tttttacttt ggcaagaaga tactactgaa
      601 gcaataaaat ttgatttcgt ggttgggaac ccgccttacg ttaggcaaga attgattcaa
      661 gatgagctga tcaaaaaata cagaaagcga tacacaacca tttatgatcg agcggattta
      721 tatgtcccat tcattcagca ctctcttgaa ctacttagtg agcaaggtac tttagggatt
      781 atttgttctg accgatttac caaaaatcga tacgggaaga agttgcgtaa atttataacc
      841 gacaactata aagttcgtta tattgttgac ctacataaaa cttctccgtt tgaaaatgag
      901 gttacagcct atccagcaat ctatgtaatc aagactaaga actatgacaa atctgtggtg
      961 cgggcagttt acaccgaagt tattacttca aaggtctgtc aagacgcaaa agatttttta
     1021 ttatctaatc aaaagccaga tcaatcttcg aaagaaatga aaacctatgt tttctctgaa
     1081 tggtttgccg gagatgagcc gtggattatt caatctcaag agtgtcgtga gatcctaaga
     1141 aggcttgaga accgatttcc gctgatcgaa gatgatgtac acagttgcaa aatcaggatc
     1201 ggtgtagcta ccggcgctga taaagtttat attgttgatc ctcaacaggt cgatattgaa
     1261 ccggaggtat tgctgccctt agtaacaact gcagatatat ctagcggtcg gatcatttgg
     1321 tctggaaagc atgttattaa cccatttaat tctgacggtg gactaattaa tttagatgat
     1381 tttccacgtc ttaagacgta ttttcaacaa catgaagaaa ttattaagaa ccgtaacgta
     1441 gcaaagaaaa atccttcgca gtggtttaga acaattgatc gtatataccc ggaaattgtt
     1501 catcaaccga agttacttat tcctgatatg aaaaacacta accatatcgt taaggacgat
     1561 ggggcttttt atcctcatca caatttatac tatatccttc cgggaaattg gaatattgac
     1621 atcctacggg ctattctatt gtcctcggtt gttaaatttt ttatatggtc atacgcgaca
     1681 aaaatgagag gagataccct tcgttatcaa gctcaatatt tgcgaaaaat ccgtcttcct
     1741 gatccgaaat cattaacaaa tgatcagaaa gaacgattaa tggacgaaag agtcattcaa
     1801 tctcaagagt acttggatag tattgtagcc gagatttatc aactatcgaa gactgaaatc
     1861 gagattatta aagatgcatt ggaataggag gatattaaag
//