From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: D.pseudoobscura Adh gene for alcohol dehydrogenase
Date: 28 Dec 92 21:27:55 GMT
Distribution: bionet
Checksum: 22057 7

LOCUS       DROADHGN70   3459 bp ds-DNA             INV       01-DEC-1992
DEFINITION  D.pseudoobscura Adh gene for alcohol dehydrogenase
ACCESSION   X64479
KEYWORDS    ADH gene; alcohol dehydrogenase.
SOURCE      Drosophila pseudoobscura DNA.
  ORGANISM  Drosophila pseudoobscura
            Eukaryota; Animalia; Metazoa; Arthropoda; Uniramia; Insecta;
            Pterygota; Neoptera; Holometabola; Diptera; Brachycera;
            Cyclorrhapha; Schizophora; Drosophiloidea; Drosophilidae.
REFERENCE   1  (bases 1 to 3459)
  AUTHORS   Schaeffer,S.W. and Miller,E.L.
  TITLE     Estimates of gene flow in Drosophila pseudoobscura determined from
            nucleotide sequence analysis of the alcohol dehydrogenase region
  JOURNAL   Genetics 132, 471-480 (1992)
  STANDARD  full automatic
COMMENT     *source: strain=PS261; *source: isolate=Kiabab National Forest,
            Arizona; *source: chromosome=IV section 88;
               From EMBL    entry DPADHGN70;  dated 16-OCT-1992.
FEATURES             Location/Qualifiers
     mRNA            join(40..119,892..997,1061..1465,1528..1943)
                     /note="adult mRNA (Adh)"
     mRNA            join(859..997,1061..1465,1528..1943)
                     /note="larval mRNA (Adh)"
     promoter        8..14
                     /note="adult promoter (Adh)"
     prim_transcript 40..1943
                     /note="adult primary transcript (Adh)"
     exon            40..119
                     /note="adult exon 1a"
     intron          120..891
                     /note="adult intron"
     prim_transcript 859..1943
                     /note="larval primary transcript (Adh)"
     exon            859..997
                     /note="larval exon 1"
     exon            892..997
                     /note="adult exon 1b"
     intron          998..1060
                     /number=1
     exon            1061..1465
                     /number=2
     intron          1466..1527
                     /number=2
     exon            1528..1943
                     /number=3
     polyA_signal    1920..1925
     polyA_site      1943
     CDS             join(905..997,1061..1465,1528..1794)
                     /EC_number="1.1.1.1"
                     /gene="Adh"
                     /product="alcoholdehydrogenase"
                     /codon_start=1
                     /translation="MSLTNKNVVFVAGLGGIGLDTSRELVKRNLKNLVILDRIDNPAA
                     IAELKAINPKVTITFYPYDVTVPVAETTKLLKTIFAQVKTIDVLINGAGILDDHQIER
                     TIAVNYTGLVNTTTAILDFWDKRKGGPGGIICNIGSVTGFNAIYQVPVYSGSKAAVVN
                     FTSSLAKLAPITGVTAYTVNPGITKTTLVHKFNSWLDVEPRVAEKLLEHPTQTSQQCA
                     ENFVKAIELNKNGAIWKLDLGTLEPITWTQHWDSGI"
BASE COUNT      972 a    791 c    786 g    910 t
ORIGIN      
        1 cgcagtgtat ttaaggcact gcacatgtcg aggaccacga ttattgtcta cagtgcagtc
       61 agtagttgtc aggtgcagca ggcggaccag catttcttta aatctactca atcgatcaag
      121 taagtaactg taatccaagt acagtcggcc catcccactc ggtcactcgg ttctgcagta
      181 attcaagaaa tttcttgata gcgcagttcc agttgtaaaa cgtcgtttaa ggaaagatcg
      241 cgctagaagt tgagttgaac gcaaagaaaa tttcaaatat aaaatatctt cccaaagcag
      301 ctgtccattt tttgttaaag tgatagatca aaaattcgta tgacgtattt cccaagtgct
      361 ctttatgcaa ataatttcct caattctaat tctacaagaa gcacttttgg ctcttgtcca
      421 gaaaaaacaa tttgtttaca cttgtcggat gataacagaa gcggaccgac ggctaagtaa
      481 cataacagtc gcctgttaag taatgccctc actctctgtt agatggccgc gcgtgtcgac
      541 tccgatagac cagcagacac gcagtagttt tcctcaagaa cctgaactct aaacatagac
      601 ataatttacg ataccttgag actcaaataa aaatacaagt atgagtggta ttctattcct
      661 gattagactc gcacacacac acgtacgagt attaacataa atttgccggc aaataaccaa
      721 aacaaaacga acgcaaacga tccgaacacg taatgcgaga gataagaaac agaaaagctc
      781 gacgtgaaag aagcttctgg cggaagctct tgccgaactc cctataaata cgagacagaa
      841 aaccagaata aatataataa caaactgttg aattgtccca tcgattccca gatcaaaaga
      901 cagaatgtct ctcaccaaca agaacgtcgt tttcgtggcc ggcctgggcg gcattggcct
      961 ggacaccagc cgggagttgg tcaagcgtaa tctgaaggta agagagaaca aattccatgg
     1021 aatctatgga atcctaaatt taaaaattaa tttattttag aacctggtca tcctggatcg
     1081 cattgacaat ccggctgcca ttgccgaact gaaggcaatc aatcccaagg tgaccatcac
     1141 cttctatccc tacgatgtga ctgtgcccgt cgctgagacc accaagctcc tgaagaccat
     1201 ctttgcccag gtgaagacaa tcgatgtcct gatcaacggt gctggcatcc tggacgatca
     1261 tcagatcgag cgcaccattg ccgttaacta cacgggcctg gtcaacacca ccacagccat
     1321 tctggacttc tgggacaagc gcaagggcgg cccaggcggc atcatttgca acattggctc
     1381 cgtcaccggt ttcaatgcca tctaccaggt gcccgtttac tccggctcca aggcggcggt
     1441 ggttaacttt acctcctccc tggcggtaag aaatcctaca actatcccaa aatccccaaa
     1501 actaaatatt tctaaatatt cccatagaaa ctggctccca ttactggtgt cactgcttac
     1561 actgtcaatc ctggcatcac taagaccact ctggtccaca aattcaactc gtggctggat
     1621 gtggagcccc gtgtggcaga gaagctgctc gagcatccca cccagacctc tcagcagtgc
     1681 gccgagaact ttgtgaaggc cattgagctg aacaagaacg gtgccatctg gaagttggat
     1741 ttgggcacct tggagcccat cacatggacc cagcactggg actcgggcat ctaaaccagc
     1801 ccagagactc tatgggacat ggcgttttag cttttagttt ctttttttcc actcaattgt
     1861 tacgtatata catacacata tggcaataag gctgatttga accccgtttt gaatatgata
     1921 atacaaaatt atatttgaga aatttcaaca aaatcgataa gagctatagc tctcttttaa
     1981 ttaaaaaaaa ctagataaaa agagcaatgg tcagtggtgg tcagtggtcg ttgtggcctc
     2041 tatcgatttc cacacaaaaa ctttactgtt tagtaataga aaagaacttg agaaaggcag
     2101 ccaaaagaat gtacgatctg acgggtaagc atgtctgcta tgtggctgac tgcggtggca
     2161 ttgcactgga gaccagcaag gttctcatga ccaagaatat agcggtgagt ggtagagtgg
     2221 tgtagagagt ggagagtggt tctggttctt aaacaagtct ctagtctcta gtctctagtc
     2281 tctagtctct tggctgctgc tggtagctgt cgaaactaat taagttaatg actttttcat
     2341 gttttgtttt tgtccgctga cttttgctgt tcaaagctac aattaagttt atgttttgag
     2401 tttgatctac ttttgcttaa gggaagtact ttcgattacg gattgctggc atataaaaat
     2461 agataccaag gaatgtgcat catatcatat tgattccatc ttgattcttt gcccagaaac
     2521 tggccatcct tcagagcgtg gaaaaccccc cggccatcgc tcagttacaa tcgattaagc
     2581 acagcacaca gatcttcttc tggaccttcg atgtgaccat ggcccgcgag gagatgaaga
     2641 agtacttcga tgaggtcatg gtacagatgg actacatcga tgtgctgatc aatggggcca
     2701 ccctgtgcga tgagcggaac attgatgcca ctatcaatac gaatctgacc ggcatgatga
     2761 acaccgtggc cactgtgctg ccctatatgg accgtaagat gggcggttcg ggtggactga
     2821 ttgtgaatgt cacctccgtc ataggactgg atccatcgcc ggtcttttgt gcgtacagtg
     2881 cctcaaagtt tggtgtgatt gggttcaccc gaagtctagc ggtgagtggg cgagatctta
     2941 acttgaaaat gctctctaat gtcttccatt ctttcgcagg atcccctgta ttacacccaa
     3001 aatggtgtgg ctgtcatggc cgtctgctgt ggtcccacca aagtgtttgt cgatcgcgaa
     3061 ctgactgcct tcctggaata cggccagtcc tttgccgatc gcctgcgtcg tgccccctgc
     3121 caatcgacgg ccgtctgtgg tcagaatata gtaaatgcca tcgagagatc ggagaacgga
     3181 cagatctgga ttgccgacaa gggcggcctg gagtcggtgg ccctccactg gtattggcat
     3241 atggccgatc agtttgtgaa ctatatgcag agcactgacg atgaagatca ggagtttttt
     3301 ttgggccagc gatgaggcat gccttgatcc acggaataaa acatcaaatg tgtcacaaaa
     3361 agtaggcaac ttgtttgtgt gtttggaaat agatttgaaa tattttgagg tcatatgaat
     3421 aatgacataa atgagggtgc ctgaaagtat gccaagttt
//