From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Manduca sexta diuretic hormone mRNA, complete cds and partial
Date: 31 Dec 92 01:46:07 GMT
Distribution: bionet
Checksum: 47555 4

LOCUS       MOTDIURHO    1130 bp ss-mRNA            INV       18-DEC-1992
DEFINITION  Manduca sexta diuretic hormone mRNA, complete cds and partial
            intron sequences.
ACCESSION   L04628 L04624 L04625 L04626 L04627
KEYWORDS    diuretic hormone; prepropeptide.
SOURCE      Manduca sexta (library: Lambda ZAPII) pharate adult head cDNA to
            mRNA.
  ORGANISM  Manduca sexta
            Eukaryota; Animalia; Metazoa; Arthropoda; Uniramia; Insecta;
            Pterygota; Neoptera; Holometabola; Lepidoptera; Glossata;
            Sphingoidea; Sphingidae.
REFERENCE   1  (bases 1 to 1130)
  AUTHORS   Digan,M.E.
  TITLE     Characterization of the precursor form for the Manduca sexta
            diuretic hormone, Mas-DH
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 89, 11074-11078 (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     sig_peptide     323..562
                     /codon_start=1
     intron          replace(442^443,"aattctatcatttaacag")
                     /number=1
                     /partial
                     /note="3' end of intron occurring here; determined from
                     genomic clone"
     intron          replace(538^539,"gtaacaagaaatatttgattgcttaca")
                     /number=2
                     /partial
                     /note="5' end of intron occurring here; determined from
                     genomic clone"
     intron          replace(538^539,"gtattgatggggcgcgaag")
                     /number=2
                     /partial
                     /note="3' end of intron occurring here; determined from
                     genomic clone"
     mat_peptide     563..688
                     /product="diuretic hormone"
                     /codon_start=1
     intron          replace(695^696,"gtacggacacttatttg")
                     /number=3
                     /partial
                     /note="5' end of intron occurring here; determined from
                     genomic clone"
     polyA_signal    1111..1116
     polyA_site      1130
     CDS             323..739
                     /product="preprodiuretic hormone"
                     /codon_start=1
                     /translation="MMWWAIWCVMVVVSSAASAAPAPDSAPMDLVQIDSAGPDDESLG
                     YAVSSLEGRYGAEAPWLYLLAEMPRDSQIGRAAVKRRMPSLSIDLPMSVLRQKLSLEK
                     ERKVHALRAAANRNFLNDIGKRGLQWSRSEQPSAYY"
BASE COUNT      331 a    227 c    223 g    349 t
ORIGIN      
        1 gaattcgacc ctaactcatt acctttcaaa taattttata aagggtgtaa agaaaaaaca
       61 caattagttt acaatatcct gtatatttac ctaagtgtaa aggaatattt acaaagtgga
      121 caacgacgta tgatgatccc taccttagtc tatttcacgt cttacataaa acagttgcag
      181 ttgtgtccaa gttttattca gtgtattttt attgctataa attttcgctg catacttttc
      241 gttagttgag aagttttcgc gttacaaaac aaaactccgt gtttccacgt agattccccg
      301 ctacttcctt ttttcttcaa caatgatgtg gtgggctatc tggtgcgtga tggtggtggt
      361 cagcagcgcg gcgtcggccg cccctgcccc tgattccgct cccatggacc tcgtgcagat
      421 agactccgcc ggacctgatg acgaaagcct cggatatgca gtatcatctt tggaaggtcg
      481 ttatggtgcc gaagctccct ggctgtactt gctcgctgaa atgccccgtg actcacagat
      541 cggcagagca gcggtgaaga ggcgcatgcc ttcgctgtcc atcgacctgc ccatgtccgt
      601 gctgcgacag aagctcagcc tcgagaagga acgcaaggtg cacgccctgc gcgccgccgc
      661 caacagaaac ttcttgaacg acattggcaa acgaggactc cagtggtcgc gatcggaaca
      721 gccatccgca tactactaga gcctgtacta ttatagtttc atgtccctaa aaatgttgat
      781 tagctcgtaa aactggtaat atgtcggaat aaatgaataa ccattgtaca cttcatacga
      841 taaaaatgaa tatttaagtt tggcgtagtg ataatgaatt ttaagaattg tatctgtatt
      901 tattaataat tgatagcaaa attgtgaaaa tatattaata attttgggcg atttaagtaa
      961 tcattattaa tttattagaa ttgcagcttg taaattgtac ttctatttat cgcaataata
     1021 tgatttactc gttgtcataa ttaaaataat atataattat ttgttttaga aaatagattt
     1081 caattatcat ctttgaatgc ataaaaatgc aataaacaat cgttgatgac
//