From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Bacillus subtilis comC gene, 5' end cds; spoIIB gene, complete cds;
Date: 6 Nov 92 18:26:31 GMT
Distribution: bionet
Checksum: 13351 6

LOCUS       BACSPOIIB    1600 bp ds-DNA             BCT       02-NOV-1992
DEFINITION  Bacillus subtilis comC gene, 5' end cds; spoIIB gene, complete cds;
            orfA gene, 3' end cds.
ACCESSION   L04519
KEYWORDS    comC gene; mutation; orfA gene; protein binding site;
            ribosome binding site; spoIIB gene; terminator.
SOURCE      Bacillus subtilis (sub_strain PY79, strain W168) DNA.
  ORGANISM  Bacillus subtilis
            Prokaryotae; Firmicutes; Endospore-forming rods and cocci;
            Bacillaceae.
REFERENCE   1  (sites)
  AUTHORS   Mohan,S., Aghion,J., Guillen,N. and Dubnau,D.
  TITLE     Molecular cloning and characterization of comC, a late competence
            gene of Bacillus subtilis
  JOURNAL   J. Bacteriol. 171, 6043-6051 (1989)
  STANDARD  full automatic
REFERENCE   2  (bases 1 to 1600)
  AUTHORS   Levin,P.A., Margolis,P.S., Setlow,P., Losick,R. and Sun,D.
  TITLE     Identification of Bacillus subtilis genes for septum placement and
            shape determination
  JOURNAL   J. Bacteriol. (1992) In press
  STANDARD  full automatic
REFERENCE   3  (bases 1 to 1600)
  AUTHORS   Margolis,P.S., Driks,A. and Losick,R.
  TITLE     Sporulation gene spoIIB from Bacillus subtilis
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     protein_bind    211..217
                     /bound_moiety="Spo0A protein"
                     /note="5 for 7 match to consensus binding site"
     protein_bind    223..229
                     /bound_moiety="Spo0A protein"
     protein_bind    236..242
                     /bound_moiety="Spo0A protein"
                     /note="6 for 7 match to consensus binding site"
     terminator      238..262
                     /gene="comC"
     RBS             326..334
                     /gene="spoIIB"
     conflict        replace(516..518,"gc")
                     /note="insertion of nucleotide 517 compared to sequence of
                     M30805"
                     /citation=[1]
     mutation        replace(707,"a")
                     /gene="spoIIB"
     terminator      1355..1381
                     /gene="spoIIB"
     stem_loop       1393..1415
     RBS             1479..1487
                     /gene="orfA"
                     /citation=[2]
     CDS             <1..209
                     /function="required for the development of genetic
                     competence"
                     /citation=[1]
                     /note="comC"
                     /gene="comC"
                     /codon_start=3
                     /translation="FVLGVKMLAAAFFFSVLIGALYGAARVLTGRLAKRQPLPFAPAI
                     AAGSILAYLYGDSIISFYIKMALG"
     CDS             343..1341
                     /function="involved in endospore development"
                     /evidence="EXPERIMENTAL"
                     /note="spoIIB"
                     /gene="spoIIB"
                     /codon_start=1
                     /translation="MKKRKNKKNSKAAEKALKVTINGKEETVYEQETPETEANKSMTF
                     SNWEEKRQAEQEVAASQEHPDEDEFNWDSEEDKVFKEDPKVVPPFQKKKTKLYAKGKT
                     GAAKPVKRVAATIAFAAVIGTGLGLFALNISGNKEASAPASLEDSLGSQTAKAGDTSA
                     DKQTSGAEKQAAQTEGTYKTYAVQAGKFSNEKGAETLTEQLTEKGYSAVSLSKDDGYT
                     YVIAGLASEKEVSQQLGQVLIDSDFEAWGGKELSLSIESDMTDSFKETAELAAKAILD
                     EDITKASVEKIEKSLGETKASETGEKKAILQALKELEDPSAEAGWKAQQELLAVVK"
     CDS             1494..1600
                     /citation=[2]
                     /partial
                     /note="orfA"
                     /gene="orfA"
                     /codon_start=1
                     /translation="MTKPLILASQSPRRKELLDLLQLPYSIIVSEVEEK"
BASE COUNT      525 a    300 c    399 g    376 t
ORIGIN      
        1 gctttgtgct tggtgtgaaa atgctggcag ctgccttttt cttttcagtt ttgataggtg
       61 cattatatgg agcggcacgt gttctgactg gtagactcgc taaaaggcag ccgcttccct
      121 tcgcccccgc tatagccgca gggagcattt tagcctattt atacggtgac tctatcattt
      181 ctttttatat caaaatggca ttgggctgac tgctgaaaaa tttgacgaac gttttttgga
      241 caaggcgaca aaagtcttgt tctttttttc tttgcctgtg ctaaagtgtg tagcatgaaa
      301 agccgacaag aacggtcaag caaatgaagg gaggaagcgg aattgaaaaa aagaaagaat
      361 aaaaaaaaca gtaaagccgc agaaaaagcc ctcaaggtga cgattaatgg aaaagaagag
      421 acggtttacg aacaagagac gcctgagaca gaggcgaata aaagcatgac attttcaaat
      481 tgggaggaaa aaagacaggc agaacaagag gttgcggcat cccaggaaca ccctgatgaa
      541 gatgaattca attgggattc cgaggaggac aaggtgttta aagaggaccc taaagttgtt
      601 cctccttttc aaaagaaaaa aacaaagctt tacgcaaaag gaaagaccgg ggctgccaaa
      661 ccggtaaaaa gagtggccgc gacgattgcc ttcgcggctg tcattggcac tggattaggg
      721 ttattcgcgt taaacatatc ggggaataaa gaagcgtcag caccggcctc tttagaggat
      781 tctttaggca gccaaacggc aaaagcaggg gatacttctg ctgataaaca aacctcaggc
      841 gcagaaaaac aagcggcaca aactgaaggt acatacaaaa catacgctgt gcaagccgga
      901 aaattttcaa atgaaaaggg cgccgaaacg ttaacagaac aattgaccga aaaaggatat
      961 tccgccgtgt cactgtcaaa ggatgacggg tatacgtatg ttattgcagg cttagcaagt
     1021 gaaaaggagg tctcacagca gttaggacag gttttaatcg acagtgattt tgaagcctgg
     1081 ggaggcaagg agctttcctt atcaatagag tcagatatga cagattcttt taaggaaaca
     1141 gcggagcttg cagcaaaggc gattctcgac gaagacatta caaaagcgag tgtagagaaa
     1201 atagaaaagt cgcttggtga aacaaaagcg tcagaaacag gagagaaaaa agcgattttg
     1261 caggctttaa aggaattaga ggatccaagt gctgaagccg ggtggaaggc tcagcaggag
     1321 ctgttagccg tcgtaaaata agagatagtg ccctgagcct tcgtccttta gtgacaggct
     1381 ttttttattt tgtcatgaaa aagttatttt tctgaaattg tgacaagcct cgtgatttgg
     1441 taggataaac gagtatcttt ttttcttaag caaaccctga aaggatgacg aacatgacaa
     1501 aaccgctaat acttgcatca caatctccgc gcaggaaaga actcctcgat cttctccagc
     1561 tgccctactc cattattgtc agtgaagtag aagaaaaatt
//