From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Poliovirus type 2 (Lansing strain), complete genome.
Date: 29 Dec 92 21:27:59 GMT
Distribution: bionet
Checksum: 65030 15

LOCUS       POL2LAN      7440 bp ss-RNA             VRL       14-DEC-1992
DEFINITION  Poliovirus type 2 (Lansing strain), complete genome.
ACCESSION   M12197
KEYWORDS    RNA polymerase; capsid protein; complete genome;
            nonstructural protein; polymerase; protease.
SOURCE      Poliovirus type 2 (Lansing strain), cDNA to viral RNA, clone
            pVR204.
  ORGANISM  Poliovirus type 2
            Viridae; ss-RNA nonenveloped viruses; Isometric ss-RNA viruses;
            Picornaviridae; Enterovirus.
REFERENCE   1  (bases 1 to 7440)
  AUTHORS   La Monica,N., Meriam,C. and Racaniello,V.R.
  TITLE     Mapping of sequences required for mouse neurovirulence of
            Poliovirus type 2 Lansing
  JOURNAL   J. Virol. 57, 515-525 (1986)
  STANDARD  full automatic
COMMENT     *M80206 is the correct accession number for the sequence
            published in:
             Journal of Virology
                   66:28072813 (1992)
                   'Molecular cloning and expression of a murine homolog
                   of the human poliovirus receptor gene'

               Computer readable copy of sequence kindly provided via
            electronic mail by V.R. Racaniello, 07-MAY-1986.
FEATURES             Location/Qualifiers
     virion          1..7440
                     /note="pv RNA"
     mat_peptide     748..954
                     /codon_start=1
                     /note="capsid protein VP4"
     mat_peptide     955..1767
                     /codon_start=1
                     /note="capsid protein VP2"
     mat_peptide     1768..2481
                     /codon_start=1
                     /note="capsid protein VP3"
     mat_peptide     2482..3384
                     /codon_start=1
                     /note="capsid protein VP1"
     mat_peptide     3385..3831
                     /codon_start=1
                     /note="protease 2A"
     mat_peptide     3832..4122
                     /codon_start=1
                     /note="nonstructural protein 2B"
     mat_peptide     4123..5109
                     /codon_start=1
                     /note="nonstructural protein 2C"
     mat_peptide     5110..5370
                     /codon_start=1
                     /note="nonstructural protein 3A"
     mat_peptide     5371..5436
                     /codon_start=1
                     /note="genome-linked protein VPg"
     mat_peptide     5437..5985
                     /codon_start=1
                     /note="protease 3C"
     mat_peptide     5986..7368
                     /codon_start=1
                     /note="RNA polymerase 3D"
     CDS             748..7371
                     /note="poliovirus polyprotein"
                     /codon_start=1
                     /translation="MGAQVSSQKVGAHENSNRAYGGSTINYTTINYYRDSASNAASKQ
                     DFAQDPSKFTEPIKDVLIKTAPTLNSPNIEACGYSDRVMQLTLGNSTITTQEAANSVV
                     AYGRWPEYIKDSEANPVDQPTEPDVAACRFYTLDTVTWRKESRGWWWKLPDALKDMGL
                     FGQNMFYHYLGRAGYTVHVQCNASKFHQGALGVFAVPEMCLAGDSTTHMFTKYENANP
                     GEKGGEFKGSFTLDTNATNPARNFCPVDYLFGSGVLAGNAFVYPHQIINLRTNNCATL
                     VLPYVNSLSIDSMTKHNNWGIAILPLAPLDFATESSTEIPITLTIAPMCCEFNGLRNI
                     TVPRTQGLPVLNTPGSNQYLTADNYQSPCAIPEFDVTPPIDIPGEVRNMMELAEIDTM
                     IPLNLTNQRKNTMDMYRVELNDAAHSDTPILCLSLSPASDPRLAHTMLGEILNYYTHW
                     AGSLKFTFLFCGSMMATGKLLVSYAPPGAEAPKSRKEAMLGTHVIWDIGLQSSCTMVV
                     PWISNTTYRQTINDSFTEGGYISMFYQTRVVVPLSTPRKMDILGFVSACNDFSVRLLR
                     DTTHISQEAMPQGLGDLIEGVVEGVTRNALTPLTPANNLPDTQSSGPAHSKETPALTA
                     VETGATNPLVPSDTVQTRHVIQKRTRSESTVESFFARGACVAIIEVDNDAPTKRASKL
                     FSVWKITYKDTVQLRRKLEFFTYSRFDMEFTFVVTSNYTDANNGHALNQVYQIMYIPP
                     GAPIPGKWNDYTWQTSSNPSVFYTYGAPPARISVPYVGIANAYSHFYDGFAKVPLAGQ
                     ASTEGDSLYGAASLNDFGSLAVRVVNDHNPTKLTSKIRVYMKPKHVRVWCPRPPRAVP
                     YYGPGVDYKDGLAPLPGKGLTTYGFGHQNKAVYTAGYKICNYHLATQEDLQNAVNIMW
                     IRDLLVVESKAQGIDSIARCNCHTGVYYCESRRKYYPVSFTGPTFQYMEANEYYPARY
                     QSHMLIGHGFASPGDCGGILRCQHGVIGIITAGGEGLVAFSDIRDLYAYEEEAMEQGV
                     SNYIESLGAAFGSGFTQQIGNKISELTSMVTSTITEKLLKNLIKIISSLVIITRNYED
                     TTTVLATLALLGCDASPWQWLKKKACDILEIPYIMRQGDSWLKKFTEACNAAKGLEWV
                     SNKISKFIDWLKEKIIPQARDKLEFVTKLKQLEMLENQIATIHQSCPSQEHQEILFNN
                     VRWLSIQSKRFAPLYAVEAKRIQKLEHTINNYVQFKSKHRIEPVCLLVHGSPGTGKSV
                     ATNLIARAIAEKENTSTYSLPPDPSHFDGYKQQGVVIMDDLNQNPDGADMKLFCQMVS
                     TVEFIPPMASLEEKGILFTSNYVLASTNSSRITPPTVAHSDALARRFAFDMDIQIMSE
                     YSRDGKLNMAMATEMCKNCHHPANFKRCCPLVCGKAIQLMDKSSRVRYSIDQITTMII
                     NERNRRSSIGNCMEALFQGPLQYKDLKIDIKTTPPPECINDLLQAVDSQEVRDYCEKK
                     GWIVDITSQVQTERNINRAMTILQAVTTFAAVAGVVYVMYKLFAGHQGAYTGLPNKRP
                     NVPTIRTAKVQGPGFDYAVAMAKRNILTATTIKGEFTMLGVHDNVAILPTHASPGETI
                     VIDGKEVEVLDAKALEDQAGTNLEITIVTLKRNEKFRDIRPHIPTQITETNDGVLIVN
                     TSKYPNMYVPVGAVTEQGYLNLSGRQTARTLMYNFPTRAGQCGGVITCTGKVIGMHVG
                     GNGSHGFAAALKRSYFTQSQGEIQWMRPSKEVGYPVINAPSKTKLEPSAFHYVFEGVK
                     EPAVLTKSDPRLKTDFEEAIFSKYVGNKITEVDEYMKEAVDHYAGQLMSLDINTEQMC
                     LEDAMYGTDGLEALDLSTSAGYPYVAMGKKKRDILNKQTRDTKEMQRLLDTYGINLPL
                     VTYVKDELRSKTKVEQGKSRLIEASSLNDSVAMRMAFGNLYAAFHKNPGVVTGSAVGC
                     DPDLFWSKIPVLMEEKLFAFDYTGYDASLSPAWFEALKMVLEKIGFGDRVDYIDYLNH
                     SHHLYKNKTYCVKGGMPSGCSGTSIFNSMINNLIIRTLLLKTYKGIDLDHLKMIAYGD
                     DVIASYPHEVDASLLAQSGKDYGLTMTPADKSATFETVTWENVTFLKRFFRADEKYPF
                     LVHPVMPMKEIHESIRWTKDPRNTQDHVRSLCLLAWHNGEEEYNKFLAKIRSVPIGRA
                     LLLPEYSTLYRRWLDSF"
BASE COUNT     2163 a   1753 c   1722 g   1802 t
ORIGIN      631 bp upstream of BalI site.
        1 ttaaaacagc tctggggttg ttcccacccc agaggcccac gtggcggcca gtacactggt
       61 attgcggtac ctttgtacgc ctgttttata ctcccttccc ccgtaactta gaagcacaat
      121 gtccaagttc aataggaggg ggtacaaacc agtaccacca cgaacaagca cttctgttcc
      181 cccggtgagg ctgtataggc tgtttccacg gctaaaagcg gctgatccgt tatccgctca
      241 tgtacttcga gaagcctagt atcaccttgg aatcttcgat gcgttgcgct caacactcaa
      301 ccccagagtg tagcttaggt cgatgagtct ggacgttcct caccggcgac ggtggtccag
      361 gctgcgttgg cggcctacct gtggcccaaa gccacaggac gctagttgtg aacaaggtgt
      421 gaagagccta ttgagctacc tgagagtcct ccggcccctg aatgcggcta atcctaacca
      481 cggagcaggc agtggcaatc cagcgaccag cctgtcgtaa cgcgcaagtt cgtggcggaa
      541 ccgactactt tgggtgtccg tgtttccttt tatttttaca atggctgctt atggtgacaa
      601 tcattgattg ttatcataaa gcaaattgga ttggccatcc ggtgagaatt tgattattaa
      661 attactctct tgttgggatt gctcctttga aatcttgtgc actcacacct attggaatta
      721 cctcattgtt aagatatcat caccactatg ggcgcccaag tctcatcaca gaaagttgga
      781 gcccatgaga attcaaacag agcttatggc ggatccacca ttaattacac tactattaat
      841 tattacaggg attctgcgag caatgccgct agtaagcagg actttgcaca agacccatcc
      901 aagttcactg aacctattaa agatgttctc attaagaccg ctcccacact aaactctcct
      961 aatatcgagg cgtgtgggta tagcgacaga gtgatgcaac taaccctagg caattccacc
     1021 attaccacac aggaggcggc caattctgtc gttgcatacg gccggtggcc cgagtacatc
     1081 aaggactcag aagcaaatcc tgtggaccag ccaactgaac cggacgttgc cgcgtgcagg
     1141 ttttacacac tagacactgt tacttggcgc aaggagtcca gagggtggtg gtggaaactg
     1201 cctgatgcac taaaggacat gggattattc ggccagaaca tgttctacca ctacctcggg
     1261 agggctggct atactgtgca cgtacagtgt aatgcttcaa agtttcacca gggcgccctc
     1321 ggggtattcg cagttccaga aatgtgcctg gcaggcgaca gcacaaccca catgtttaca
     1381 aaatatgaga atgcaaatcc gggtgagaaa gggggtgaat tcaaagggag ttttactctg
     1441 gatactaacg ctaccaaccc tgcacgcaac ttttgtcccg ttgattatct cttcgggagc
     1501 ggagtactgg cgggaaatgc gtttgtttac ccacatcaga taattaatct gcgcaccaac
     1561 aactgtgcca cgttggtgct gccatacgtt aattcacttt ccatagacag catgacaaaa
     1621 cacaacaatt ggggaattgc tatccttccg ctggcaccac ttgactttgc caccgagtcc
     1681 tccactgaga tacccattac tctaactatt gcccctatgt gttgtgaatt caatgggttg
     1741 cgcaacatca ctgtacccag aactcaaggg ttgccagtct taaacactcc aggaagcaac
     1801 cagtacttaa cagcagacaa ctatcaatcc ccatgtgcga tacccgagtt tgatgtaaca
     1861 ccacccatag acatcccggg ggaagtgcgc aacatgatgg aattggcaga gatagacacc
     1921 atgatacctc tcaatctgac gaaccagcgc aagaacacca tggatatgta cagagtcgaa
     1981 ctgaatgatg cggctcactc tgacacacca atattgtgtc tctcactgtc tccagcatca
     2041 gatcctaggc tagcacacac tatgctaggt gaaatactga actactacac acactgggca
     2101 gggtcattga agttcacatt tctcttctgc ggctcaatga tggccactgg taaattgcta
     2161 gtgtcctatg cacctcctgg tgcggaagcc cctaaaagcc gcaaagaagc gatgctcggc
     2221 acccacgtga tctgggacat cggattacag tcatcatgca ctatggtggt accttggatt
     2281 agcaacacca catacagaca aaccatcaac gatagcttca cagaaggagg gtacatcagt
     2341 atgttttacc aaactagagt tgttgtgcca ttgtccaccc ctagaaagat ggacatattg
     2401 ggctttgtgt cagcctgcaa tgacttcagt gtgcgcctgt tgcgtgacac gacgcacata
     2461 agccaagagg ctatgccaca aggattgggt gatttaattg aaggggttgt tgagggagtc
     2521 acgagaaatg ccttgacacc actgacacct gccaacaact tgcctgatac acaatctagc
     2581 ggcccagccc actctaagga aacaccagcg ctaacagccg tagagacagg ggccaccaac
     2641 ccattggtgc cttcagacac ggtacaaact cgtcacgtca tccaaaagcg gacgcggtcg
     2701 gagtctacgg ttgagtcttt cttcgcaaga ggagcttgtg tggccattat tgaagtggat
     2761 aatgatgctc caacaaagcg tgccagtaaa ttattttcag tctggaagat aacttacaaa
     2821 gacaccgttc agttaagacg taagttggag ttctttacat attcaaggtt tgacatggag
     2881 ttcacctttg tggttacatc caattatacc gatgcaaaca atgggcacgc actaaatcaa
     2941 gtttaccaga taatgtacat accacctggg gcaccgatcc ctggcaagtg gaatgattac
     3001 acatggcaaa cgtcatctaa cccatcagtg ttttacactt acggggcacc tccagctaga
     3061 atatcagtgc cctacgtggg cattgccaat gcatattctc atttttacga tgggtttgcc
     3121 aaagtaccac tagcaggcca agcctcaaca gagggtgact cgctgtatgg agcggcttca
     3181 ttgaatgact tcggatcact ggctgttcga gtggtgaatg accacaaccc tacgaaactc
     3241 acctcaaaaa tcagagtgta catgaaacca aagcacgtca gagtgtggtg tccgcgaccc
     3301 cctcgagcag tcccatacta cggaccaggg gttgactaca aggatggact agccccactg
     3361 ccagggaaag gcttgacaac ctatggtttt ggccaccaaa ataaggcagt gtacacggca
     3421 ggttacaaaa tttgcaatta ccacctcgcc acccaggaag acttacaaaa tgcggtaaac
     3481 attatgtgga ttagagacct tttagtagtg gaatccaaag cccaaggcat agactcaatt
     3541 gctagatgta actgccacac tggagtgtac tactgtgaat ccaggaggaa gtactacccg
     3601 gtctctttta ctggccccac ctttcagtac atggaagcaa atgagtacta tccagcccga
     3661 taccaatccc acatgttaat tggccatggt tttgcatctc caggggactg tggtgggatt
     3721 ctcaggtgcc aacatggagt aattggaatc attacagctg gaggagaagg cctagtcgct
     3781 ttctcggaca tcagagatct gtacgcatac gaggaggagg ctatggagca gggagtctcc
     3841 aactatattg agtcccttgg ggctgcattt gggagtggat tcacccagca aataggaaac
     3901 aaaatttcag aactcactag catggtcacc agcactataa ctgagaaact actaaagaat
     3961 ctcattaaaa taatttcatc ccttgttatc atcaccagaa actatgaaga cacgaccaca
     4021 gtgctggcta cccttgctct cctcggttgt gatgcgtccc catggcaatg gctaaagaag
     4081 aaagcctgtg acatcttgga aatcccctac atcatgcgac agggcgatag ctggttgaag
     4141 aagtttacag aggcatgcaa tgcagctaag ggattggaat gggtgtctaa taaaatatcc
     4201 aaatttattg actggctcaa agagaagatc attccacagg ctagagacaa gctagagttt
     4261 gttaccaaac tgaagcaact agaaatgttg gagaaccaaa ttgcaaccat tcatcaatcg
     4321 tgcccaagtc aggagcatca agaaatcctg ttcaataacg tgagatggtt atccatacag
     4381 tcaaagagat ttgccccgct ctatgcggtt gaggctaaga gaatacaaaa gttagagcac
     4441 acgattaaca actacgtaca gttcaagagc aaacaccgta ttgaaccagt atgtttgttg
     4501 gtgcacggta gcccaggcac gggcaagtca gttgccacca atttaattgc cagagcaata
     4561 gcagagaagg agaacacctc cacatactca ctaccaccag atccctccca tttcgatggg
     4621 tacaagcaac aaggtgtggt gatcatggat gatttgaatc agaacccaga cggagcagac
     4681 atgaagctgt tttgtcagat ggtctccact gtagaattca taccaccaat ggcttcgcta
     4741 gaagaaaagg gtattttgtt cacatctaat tacgttttgg cctcaaccaa ttccagtcgc
     4801 atcaccccac caactgttgc gcacagcgat gccctagcca ggcgctttgc atttgacatg
     4861 gacatacaaa tcatgagcga gtattctaga gatggaaaat tgaacatggc gatggcaact
     4921 gaaatgtgta agaactgtca tcacccagca aacttcaaga gatgttgccc attggtgtgt
     4981 ggcaaagcca tccagctgat ggacaaatct tccagagtca gatatagtat agatcagatt
     5041 actaccatga ttattaatga gaggaacaga agatcaagta tcggtaattg catggaggca
     5101 cttttccaag gtcctcttca atacaaagac ctgaaaatag acattaagac cacacctcct
     5161 cctgagtgca tcaatgattt gctccaagca gttgattctc aagaggtaag agactactgt
     5221 gagaagaagg gttggatagt agacatcact agtcaggtgc aaaccgaaag aaacatcaat
     5281 agagcaatga ctattcttca ggcggtcacc acatttgccg cagttgctgg agtggtgtat
     5341 gtgatgtaca aactctttgc agggcatcaa ggagcgtata cagggcttcc caataagaga
     5401 cccaatgtcc ccaccatcag gactgccaag gttcagggcc caggatttga ctacgcagtg
     5461 gcaatggcca aaagaaacat tcttacggca actaccatta agggagagtt cacaatgctc
     5521 ggagtgcatg ataatgtggc cattctacca acccacgcat caccgggtga aacaatagtc
     5581 attgatggca aggaagtaga ggtactggat gctaaagccc tggaggacca ggccgggacc
     5641 aacctggaaa tcaccattgt cactcttaag agaaatgaga agttcaggga catcagacca
     5701 cacatcccca ctcaaatcac tgagacaaat gatggagttt taattgtgaa cactagtaag
     5761 taccccaaca tgtatgttcc tgtcggtgct gtgactgaac aggggtatct caatctcagt
     5821 ggacgccaaa ctgctcgtac tttaatgtac aactttccaa cgagagcagg tcaatgtggt
     5881 ggagttatca cctgcactgg caaggtcatc gggatgcatg ttggtgggaa cggttcacat
     5941 gggttcgcag cagccctgaa gcgatcctat ttcactcaga gtcaaggtga aatccagtgg
     6001 atgagaccat caaaagaagt gggctacccc gttattaatg ctccatctaa aactaaactg
     6061 gaacccagtg cattccatta tgtgtttgaa ggtgtcaagg aaccagctgt gctcaccaaa
     6121 agtgacccca gattgaagac agattttgaa gaggctatct tttccaagta tgtgggaaat
     6181 aagattactg aagtggatga gtacatgaaa gaagctgtcg atcattacgc aggccagctc
     6241 atgtcactag acatcaacac agaacaaatg tgccttgagg atgcaatgta tggcactgac
     6301 ggtctcgaag ctctagacct cagtaccagt gctgggtatc cctatgtggc aatggggaaa
     6361 aagaaaagag acattttgaa taagcaaacc agagacacaa aggaaatgca aaggcttctg
     6421 gacacctatg gtattaattt acctttagtc acctatgtga aagatgagct tagatccaag
     6481 accaaagtgg aacagggcaa gtccaggcta attgaggcct caagtctcaa tgactctgtc
     6541 gccatgagga tggcttttgg caacttgtac gcagcattcc acaagaaccc aggtgtagtg
     6601 acaggatcgg ctgttggctg tgacccagat ttgttttgga gtaaaatacc agtcctcatg
     6661 gaggaaaaac tctttgcatt tgattacacg ggttatgatg cttcactaag ccccgcctgg
     6721 tttgaggctc tcaagatggt tctagagaaa attgggtttg gtgacagagt ggattacatt
     6781 gattatctga atcactcgca ccatctatat aaaaataaga catattgtgt taagggcggc
     6841 atgccatctg gctgctctgg cacctcaatt tttaattcaa tgattaataa tctaataatc
     6901 aggactctct tactgaaaac ctacaagggc atagatttag accacctgaa gatgatagcc
     6961 tatggtgatg atgtaattgc ttcctacccc catgaggttg atgctagtct cctagcccaa
     7021 tcaggaaaag actatggact aaccatgaca ccagctgaca aatcagccac ctttgaaaca
     7081 gtcacatggg agaatgtaac attcttgaaa agattcttta gagcagatga aaagtatccc
     7141 tttctggtac atccagtgat gccaatgaaa gaaattcacg aatcaattag atggactaaa
     7201 gatcccagaa acactcagga tcatgttcgc tcactgtgct tattggcttg gcacaatggc
     7261 gaggaagagt acaataaatt tttagctaag attagaagtg tgccaatcgg aagagcatta
     7321 ctgctccctg agtactccac attgtaccgc cgttggctcg actcatttta gtaaccctac
     7381 ctcagtcgaa ttggattggg tcatactgtt gtaggggtaa atttttcttt aattcggagg
//