From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Plasmid pTiS4 (from Agrobacterium tumefaciens) T1 vitopine synthase
Date: 22 Dec 92 22:45:48 GMT
Distribution: bionet
Checksum: 56717 7

LOCUS       IS4T1OPINE   3720 bp ds-DNA             BCT       23-NOV-1992
DEFINITION  Plasmid pTiS4 (from Agrobacterium tumefaciens) T1 vitopine synthase
            gene, complete cds.
ACCESSION   M91608
KEYWORDS    vitopine synthase.
SOURCE      Plasmid pTiS4 DNA.
  ORGANISM  Plasmid pTiS4
            Prokaryotae.
REFERENCE   1  (bases 1 to 3720)
  AUTHORS   Canaday,J., Gerard,J.-C., Crouzet,P. and Otten,L.
  TITLE     Organization and functional analysis of three T-DNAs from the
            vitopine Ti Plasmid pTiS4
  JOURNAL   Mol. Gen. Genet. (1992) In press
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     TATA_signal     831..836
                     /note="6b ORF TATA-box"
     polyA_signal    1628..1633
     polyA_signal    complement(1948..1952)
     TATA_signal     complement(3182..3188)
     enhancer        complement(3400..3415)
                     /note="octopine synthase enhancer-like sequence"
     CDS             884..1504
                     /function="tumor formation and increases auxin and
                     cytokinin effects in host plants."
                     /note="ORF 6b"
                     /codon_start=1
                     /translation="MAVPRWAVRDLTQISSARELSLRLEQARNDFRATVGSICYFNAS
                     ARTPGQFDDEYIMTDQSLTYVYADGVTAQSCAMNRLLPSSSSNFGAAATAIPPWLLDP
                     PRLNRLLREGTDEGGLVNYYEGPHKNAFFLAIMRSCIFVRPGADEINGVSYDFFARSG
                     NYTEQAEEEEEEEEEEEEEEVLDREFQLGNLVSYPIIAWGSCPNSA"
     CDS             complement(2044..3126)
                     /function="opine synthesis"
                     /product="vitopine synthase"
                     /codon_start=1
                     /translation="MSKVAILGAGNLALTFAGDIARRLNEEVTAVIWAPTTNRRNFNE
                     VRGIGTLELVGPDYEGAFTPQLEDDLEAAILDAEFIFLTVPTLAQEGILRELVKFDLS
                     ETVLIALPGSATSLTCKNILFPSHSPVAVIESTTSPYACRRIGERVHMLGVKACFEVA
                     ATSLLSNDLTSRFEALFPNRLQWYKDAASIFFSNTNPVVHPPGILVAKDAIENGMSPL
                     PKFYREFVPAAIERVQELDNERLEIIAALGLESETGFTYSKKWYGGQATEWKEFFETY
                     EGYAEVGTPTTMHHRYLTEDVKHIMVLWVQIAEVAGVQVPAMKSVIKEAGNVLYEDLF
                     MTGRTLASMNLGGANPYRIVDALNGN"
BASE COUNT      931 a    864 c    920 g   1005 t
ORIGIN      10.2 kb left of T-DNA T2
        1 ctgcaggagg tcctcaaccg ctcgccgatc ataacatcaa ccggattgac gggctcttaa
       61 cgtggaacgt gcaagatccg ttagaaatgg cctgaccgga cgcttacttt aaacgctcag
      121 cccgatgtga cactcaatgc cggagaatag gcgagcgctt ttatccggca ctctcgcgcc
      181 gacgcgattg gcacgcgagc gatcgtcatt gccgcggtga caacggcggc ggcaggatat
      241 atttagttgt aaaaaaatca gacgtacttt cagtagttgc taccgccctc agaaatttga
      301 ttttgaaaaa acatacctgc ttctaagatt atcacttact gaatgatgcc caatgtcagt
      361 gtatattaca atcgacttgc gcaattttcc aagatcaagc acgcaatttt tgaaattgct
      421 tgtcagttct ctgaagaatt ctgatcactc catgatttcc gctgattgat tgggtgtccc
      481 ttggacctaa ttgctgcaca tttttgactt tactagcggg cggccgaatc tgttcacact
      541 gcggcgtgct tgatttgagc cttttgccaa agggcggtgc tgacgctgtc actgacattc
      601 gaaccgcgtg gcccccgaag aattgtcttg agcaacacgt cgctttctca actgacgccg
      661 tcaaagtctt tgggtaaggg cgtatccaaa agaatcaaaa gtgcgctgag acattgtcaa
      721 ctaggacggc gatgcatgaa atcagggcga tgattgagct agtatcatgg aaagtcacac
      781 tgctccgtag tcttcttgcg aaatgcccaa ctcttgcgtt ggcacacttc tataaatacg
      841 tcggatgaac cgtggtgcta tctcaaggca tttcgacatt gctatggcgg taccgagatg
      901 ggctgttcgt gacctaactc aaatatcgag tgctcgggaa ctctcacttc gcttggagca
      961 ggcccgcaac gatttccggg ccacggtagg atcaatctgt tacttcaacg cttcggcgcg
     1021 tactcctggt caatttgacg atgaatatat aatgaccgat caatctttga cttacgttta
     1081 tgctgatgga gtgactgccc aaagctgcgc tatgaaccgc cttctaccct ccagttcaag
     1141 taatttcggc gctgcggcaa cagccatccc cccgtggctg ttggatccgc cgcggttgaa
     1201 ccgcctcctt cgagagggaa ctgacgaagg gggcctagtc aattactatg agggcccaca
     1261 taaaaacgca ttctttctcg ctatcatgcg aagctgcata ttcgtacgtc ctggcgctga
     1321 tgagatcaat ggtgtatctt acgacttctt tgcaagaagt ggcaactaca ccgaacaagc
     1381 agaagaggaa gaggaagagg aagaggaaga ggaagaggaa gaagtgctag accgagagtt
     1441 tcagttgggc aatctagtca gctatccgat catcgcttgg ggctcatgtc ctaattctgc
     1501 ttaacctctc gcttggtggg gatgggcctc catttcagtt gccgtaggcc tgcctatctg
     1561 caaaatttgc caagaatatt tactgctctc tactattaaa gcgtacaaca tcacttttat
     1621 atagttaaat aaaacttgta cgcagtgtgt caattgtgat ttcggttggt tgtgtcatgt
     1681 agttttctgg tgtaagtgcg acatattatt gttgccagta atttaatatg catgttttat
     1741 aaacgttatt atgttcttgt tggcgtatct gatatttatt cgcgctaagt gaaatttaga
     1801 agcattaata tcgattgttg gtgtattttt tcgtttattt attttttaag ttatttaaaa
     1861 acaaattttt ccgtgaattt caactaagga aatacagcat attttacaat atcacatatt
     1921 atacagtggc gccgggcatg ccagacctta ttcaagcctc tcacaagaca agaaataaaa
     1981 acacacgctg gatgcttcag tgcatcataa tatattacat cagatcaagc tggaagacgg
     2041 gtttcagttt ccattgagcg cgtcgacaat acgatatggg ttagcgccac ctaagttcat
     2101 tgaggcaaga gtcctgccag tcataaagag gtcctcgtag agaacgttcc cggcctcctt
     2161 tatgacagac ttcatcgctg gaacctgcac tccggccact tctgctatct gaacccagag
     2221 caccatgatg tgtttgacat cttcagttaa gtagcggtga tgcattgttg tgggtgtccc
     2281 gacttcagca taaccttcgt aggtctcaaa gaattccttc cactcggtgg cctggccgcc
     2341 ataccacttt ttagaatatg tgaaacctgt ttcggactcg aggccgagcg ccgcaataat
     2401 ctcgaggcgt tcattgtcta attcctgaac acgctcgatg gctgccggaa caaactccct
     2461 gtagaattta ggtagagggc tcataccatt ttcaatcgca tctttcgcca ccaggatccc
     2521 tggaggatgc acaaccggat tggtatttga gaaaaatatg gaggcagcat ccttgtacca
     2581 ctgcagtcgg tttgggaaaa gggcctcaaa acgacttgtg agatcgttgc tcagaaggct
     2641 tgttgcggca acctcgaagc aggctttcac gccaagcata tgaacgcgtt cgccgatacg
     2701 gcggcaggcg tagggcgacg ttgttgattc aataacagca acgggtgaat ggcttgggaa
     2761 caagatattc ttacaagtca gggaagttgc actacctggc aacgcgatga gcacagtttc
     2821 gctcagatcg aacttcacca actcgcgcag aattccctcc tgcgccagag tcggtaccgt
     2881 tagaaaaatg aactcagcgt ccaagattgc cgcttcaaga tcatcctcca actggggagt
     2941 aaaagcgcct tcatagtcgg ggccgaccag ctccaaggtt ccgatacccc tgacttcatt
     3001 gaaatttctt cggttggtgg ttggtgccca gataacagct gtgacttcct cgttgagcct
     3061 tcgagcgatg tctccagcaa acgttagagc aaggttcccc gcaccgagaa ttgctacttt
     3121 ggacattgtg agattggttt tgaagaagtt ttgaggtgag ggttccctcg ttctttggcc
     3181 cttaaatagg gagaaggggg gtgggtccct tcaagttgcc agcacagctg tgttgaattt
     3241 ctcggataga taggtcactg cgtacgtagg aagcttgaat ttcatgcctt caattattta
     3301 tctgctgagc gcagttgatg aaaaaatgga cccgagaaag cgatgttgga cacgcggcgt
     3361 ccttaactac ctatcattac gggttaggtg cttagatgta cgtaagcgcc tacgcacgtt
     3421 taccgttacg ttatttggct gtaagcagtg acgtatgaca ggatttatcg ttatgtcatg
     3481 ttcgttaatt cgtgcattca tataatagtt tgcatgtatc taagctgttg acgatgcggt
     3541 gcgcgacgtg gtgctcacga tccattggaa gggcggtcac gactcggagc tgagagttcg
     3601 caagcctcag agcggtgaac acgggtgcgc cacaacggaa gacgccttgg cagtgatgcg
     3661 gacagtggct ggacgctggt cgatgaacat atcgccgcgt cgctcaatcg aatgggcatg
//