From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Clostridium botulinum neurotoxin type F gene, complete cds.
Date: 12 Sep 92 10:02:13 GMT
Distribution: bionet
Checksum: 36203     8

LOCUS       CLONEUTOXF   4234 bp ds-DNA             BCT       12-SEP-1992
DEFINITION  Clostridium botulinum neurotoxin type F gene, complete cds.
ACCESSION   M92906
KEYWORDS    neurotoxin type F.
SOURCE      Clostridium botulinum (strain 202F) (library: ATCC 23387) DNA.
  ORGANISM  Clostridium botulinum
            Prokaryotae; Firmicutes; Endospore-forming rods and cocci;
            Bacillaceae.
REFERENCE   1  (bases 1 to 4234)
  AUTHORS   East,A.K., Richardson,P.T., Allaway,D., Collins,M.D., Roberts,T.A.
            and Thompson,D.E.
  TITLE     Sequence of the gene encoding type F neurotoxin of Clostridium
            botulinum
  JOURNAL   Unpublished (1992)
  STANDARD  full staff_entry
FEATURES             Location/Qualifiers
     RBS             173..178
     CDS             186..4010
                     /product="neurotoxin type F"
                     /codon_start=1
                     /translation="MPVAINSFNYNDPVNDDTILYMQIPYEEKSKKYYKAFEIMRNVW
                     IIPERNTIGTNPSDFDPPASLKNGSSAYYDPNYLTTDAEKDRYLKTTIKLFKRINSNP
                     AGKVLLQEISYAKPYLGNDHTPIDEFSPVTRTTSVNIKLSTNVESSMLLNLLVLGAGP
                     DIFESCCYPVRKLIDPDVVYDPSNYGFGSINIVTFSPEYEYTFNDISGGHNSSTESFI
                     ADPAISLAHELIHALHGLYGARGVTYEETIEVKQAPLMIAEKPIRLEEFLTFGGQDLN
                     IITSAMKEKIYNNLLANYEKIATRLSEVNSAPPEYDINEYKDYFQWKYGLDKNADGSY
                     TVNENKFNEIYKKLYSFTESDLANKFKVKCRNTYFIKYEFLKVPNLLDDDIYTVSEGF
                     NIGNLAVNNRGQSIKLNPKIIDSIPDKGLVEKIVKFCKSVIPRKGTKAPPRLCIRVNN
                     SELFFVASESSYNENDINTPKEIDDTTNLNNNYRNNLDEVILDYNSQTIPQISNRTLN
                     TLVQDNSYVPRYDSNGTSEIEEYDVVDFNVFFYLHAQKVPEGETNISLTSSIDTALLE
                     ESKDIFFSSEFIDTINKPVNAALFIDWISKVIRDFTTEATQKSTVDKIADISLIVPYV
                     GLALNIIIEAEKGNFEEAFELLGVGILLEFVPELTIPVILVFTIKSYIDSYENKNKAI
                     KAINNSLIEREAKWKEIYSWIVSNWLTRINTQFNKRKEQMYQALQNQVDAIKTAIEYK
                     YNNYTSDEKNRLESEYNINNIEEELNKKVSLAMKNIERFMTESSISYLMKLINEAKVG
                     KLKKYDNHVKSDLLNYILDHRSILGEQTNELSDLVTSTLNSSIPFELSSYTNDKILII
                     YFNRLYKKIKDSSILDMRYENNKFIDISGYGSNISINGNVYIYSTNRNQFGIYNSRLS
                     EVNIAQNNDIIYNSRYQNFSISFWVRIPKHYKPMNHNREYTIINCMGNNNSGWKISLR
                     TVRDCEIIWTLQDTSGNKENLIFRYEELNRISNYINKWIFVTITNNRLGNSRIYINGN
                     LIVEKSISNLGDIHVSDNILFKIVGCDDETYVGIRYFKVFNTELDKTEIETLYSNEPD
                     PSILKNYWGNYLLYNKKYYLFNLLRKDKYITLNSGILNINQQRGVTEGSVFLNYKLYE
                     GVEVIIRKNGPIDISNTDNFVRKNDLAYINVVDRGVEYRLYADTKSEKEKIIRTSNLN
                     DSLGQIIVMDSIGNNCTMNFQNNNGSNIGLLGFHSNNLVASSWYYNNIRRNTSSNGCF
                     WSSISKENGWKE"
BASE COUNT     1710 a    442 c    684 g   1398 t
ORIGIN
        1 gcaagtaaat ttattattag taatgatata tttatttcaa attgtttaac gcatgctcat
       61 aacaataagt atatatgttt atctatgaaa gatgagaact ataattggat gatatgtaac
      121 aatgaaagca acatacctaa aaaggcatat ttgtggatat tgaaagaagt atagggggga
      181 tttttatgcc agttgcaata aatagtttta attataatga ccctgttaat gatgatacaa
      241 ttttatacat gcagatacca tatgaagaaa aaagtaaaaa atattataaa gcttttgaga
      301 ttatgcgtaa tgtttggata attcctgaga gaaatacaat aggaacgaat cctagtgatt
      361 ttgatccacc ggcttcatta aagaacggaa gcagtgctta ttatgatcct aattatttaa
      421 ccactgatgc tgaaaaagat agatatttaa aaacaacgat aaaattattt aagagaatta
      481 atagtaatcc tgcagggaaa gttttgttac aagaaatatc atatgctaaa ccatatttag
      541 gaaatgacca cacgccaatt gatgaattct ctccagttac tagaactaca agtgttaata
      601 taaaattatc aactaatgtt gaaagttcaa tgttattgaa tcttcttgta ttgggagcag
      661 gacctgatat atttgaaagt tgttgttacc ccgttagaaa actaatagat ccagatgtag
      721 tttatgatcc aagtaattat ggttttggat caattaatat cgtgacattt tcacctgagt
      781 atgaatatac ttttaatgat attagtggag ggcataatag tagtacagaa tcatttattg
      841 cagatcctgc aatttcacta gctcatgaat tgatacatgc actgcatgga ttatacgggg
      901 ctaggggagt tacttatgaa gagactatag aagtaaagca agcacctctt atgatagccg
      961 aaaaacccat aaggctagaa gaatttttaa cctttggagg tcaggattta aatattatta
     1021 ctagtgctat gaaggaaaaa atatataaca atcttttagc taactatgaa aaaatagcta
     1081 ctagacttag tgaagttaat agtgctcctc ctgaatatga tattaatgaa tataaagatt
     1141 attttcaatg gaagtatggg ctagataaaa atgctgatgg aagttatact gtaaatgaaa
     1201 ataaatttaa tgaaatttat aaaaaattat atagttttac agagagtgac ttagcaaata
     1261 aatttaaagt aaaatgtaga aatacttatt ttattaaata tgaattttta aaagttccaa
     1321 atttgttaga tgatgatatt tatactgtat cagaggggtt taatataggt aatttagcag
     1381 taaacaatcg cggacaaagt ataaagttaa atcctaaaat tattgattcc attccagata
     1441 aaggtctagt agaaaagatc gttaaatttt gtaagagcgt tattcctaga aaaggtacaa
     1501 aggcgccacc gcgactatgc attagagtaa ataatagtga gttatttttt gtagcttcag
     1561 aaagtagcta taatgaaaat gatattaata cacctaaaga aattgacgat acaacaaatc
     1621 taaataataa ttatagaaat aatttagatg aagttatttt agattataat agtcagacaa
     1681 tacctcaaat atcaaatcga acattaaata cacttgtaca agacaatagt tatgtgccaa
     1741 gatatgattc taatggaaca agtgaaatag aggaatatga tgttgttgac tttaatgtat
     1801 ttttctattt acatgcacaa aaagtgccag aaggtgaaac caatataagt ttaacttctt
     1861 caattgatac agcattatta gaagaatcca aagatatatt tttttcttca gagtttatcg
     1921 atactatcaa taaacctgta aatgcagcac tatttataga ttggataagc aaagtaataa
     1981 gagattttac cactgaagct acacaaaaaa gtactgttga taagattgca gacatatctt
     2041 taattgtacc ctatgtaggt cttgctttga atataattat tgaggcagaa aaaggaaatt
     2101 ttgaggaggc atttgaatta ttaggagtgg gtattttatt agaatttgtg ccagaactta
     2161 caattcctgt aattttagtg tttacgataa aatcctatat agattcatat gagaataaaa
     2221 ataaagcaat taaagcaata aataattcat taatcgaaag agaagcaaag tggaaagaaa
     2281 tatatagttg gatagtatca aattggctta ctagaattaa tactcaattt aataaaagaa
     2341 aagagcaaat gtatcaggct ttacaaaatc aagtagatgc aataaaaaca gcaatagaat
     2401 ataaatataa taattatact tcagatgaga aaaatagact tgaatctgaa tataatatca
     2461 ataatataga agaagaattg aataaaaaag tttctttagc aatgaaaaat atagaaagat
     2521 ttatgacaga aagttctata tcttatttaa tgaaattaat aaatgaagcc aaagttggta
     2581 aattaaaaaa atatgataac catgttaaga gcgatttatt aaactatatt ctcgaccata
     2641 gatcaatctt aggagagcag acaaatgaat taagtgattt ggtgactagt actttgaata
     2701 gtagtattcc atttgaactt tcttcatata ctaatgataa aattctaatt atatatttta
     2761 atagattata taaaaaaatt aaagatagtt ctattttaga tatgcgatat gaaaataata
     2821 aatttataga tatctctgga tatggttcaa atataagcat taatggaaac gtatatattt
     2881 attcaacaaa tagaaatcaa tttggaatat ataatagtag gcttagtgaa gttaatatag
     2941 ctcaaaataa tgatattata tacaatagta gatatcaaaa ttttagtatt agtttctggg
     3001 taaggattcc taaacactac aaacctatga atcataatcg ggaatacact ataataaatt
     3061 gtatggggaa taataattcg ggatggaaaa tatcacttag aactgttaga gattgtgaaa
     3121 taatttggac tttacaagat acttctggaa ataaggaaaa tttaattttt aggtatgaag
     3181 aacttaatag gatatctaat tatataaata aatggatttt tgtaactatt actaataata
     3241 gattaggcaa ttctagaatt tacatcaatg gaaatttaat agttgaaaaa tcaatttcga
     3301 atttaggtga tattcatgtt agtgataata tattatttaa aattgttggt tgtgatgatg
     3361 aaacgtatgt tggtataaga tattttaaag tttttaatac ggaattagat aaaacagaaa
     3421 ttgagacttt atatagtaat gagccagatc caagtatctt aaaaaactat tggggaaatt
     3481 atttgctata taataaaaaa tattatttat tcaatttact aagaaaagat aagtatatta
     3541 ctctgaattc aggcatttta aatattaatc aacaaagagg tgttactgaa ggctctgttt
     3601 ttttgaacta taaattatat gaaggagtag aagtcattat aagaaaaaat ggtcctatag
     3661 atatatctaa tacagataat tttgttagaa aaaacgatct agcatacatt aatgtagtag
     3721 atcgtggtgt agaatatcgg ttatatgctg atacaaaatc agagaaagag aaaataataa
     3781 gaacatctaa tctaaacgat agcttaggtc aaattatagt tatggattca ataggaaata
     3841 attgcacaat gaattttcaa aacaataatg ggagcaatat aggattacta ggttttcatt
     3901 caaataattt ggttgctagt agttggtatt ataacaatat acgaagaaat actagcagta
     3961 atggatgctt ttggagttct atttctaaag agaatggatg gaaagaatga taggccacag
     4021 taattaatct caaactacat gagtctgtca agaatttttg tataaacatt cataaaaaat
     4081 tttaaaatta atatatttaa gaatagctag atatgagtat tgctatgtta atatctagct
     4141 attttaattt atcccatatt attataataa gaaaaaatac tatttctatt gtaagtacaa
     4201 gtttattggt atctcataaa tgatacaaga tatc
//