From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: R.norvegicus (strain Wistar) b5R gene for NADH-cytochrome b5
Date: 3 Jan 93 22:08:32 GMT
Distribution: bionet
Checksum: 08087 4

LOCUS       RATB5RDNA    1133 bp ds-DNA             ROD       21-DEC-1992
DEFINITION  R.norvegicus (strain Wistar) b5R gene for NADH-cytochrome b5
            reductase (erythroid-specific exon 2)
ACCESSION   X65190
KEYWORDS    NADH-cytochrome b5 reductase; b5R gene;
            erythroid transcription factor GATA-1; erythroid-specific isoform.
SOURCE      Rattus norvegicus DNA.
  ORGANISM  Rattus norvegicus
            Eukaryota; Animalia; Chorqata; Vertebrata; Mammalia; Theria;
            Eutheria; Rodentia; Myomorpha; Muridae; Murinae.
REFERENCE   1  (bases 1 to 1133)
  AUTHORS   Pietrini,G., Aggujaro,D., Carrera,P., Malyszko,J., Vitale,A. and
            Borgese,N.
  TITLE     A single mRNA, transcribed from an alternative, erythroid-specific,
            promoter, codes for 2 non-myristylated forms of NADH-cytochrome b5
            reductase
  JOURNAL   J. Cell Biol. 117, 975-986 (1992)
  STANDARD  full automatic
COMMENT     *source: strain=Wistar; *source: tissue=liver; *source:
            clone_library=liver genomic in lambda Charon 35; *source: clone=B2,
            3;
               From EMBL    entry RNB5RDNA;  dated 05-OCT-1992.
FEATURES             Location/Qualifiers
     promoter        1..212
     CAAT_signal     104..112
     repeat_region   138..143
                     /rpt_type=inverted
     misc_feature    complement(147..152)
                     /note="GATA-1 recognition sequence"
     repeat_region   149..154
                     /rpt_type=inverted
     CAAT_signal     154..162
     TATA_signal     186..191
     mRNA            213..287
                     /gene="b5R"
     prim_transcript 213..>1133
     5'UTR           213..248
     exon            213..287
                     /number=2
     intron          288..1133
                     /number=2
     misc_feature    306..311
                     /note="GATA-1 recognition sequence"
     misc_signal     370..381
                     /note="GT box"
     CDS             249..287
                     /EC_number="1.6.2.2"
                     /gene="b5R"
                     /partial
                     /product="cytochrome b5 reductase"
                     /codon_start=1
                     /translation="MLGPLLWTASLPV"
BASE COUNT      210 a    291 c    349 g    283 t
ORIGIN      
        1 ccatggcctc atctgtccaa cagtcgctcg gtactgtctt tgatttatgg aactgactgg
       61 ggtcttacag tcctcagtgc ccacgatgca tattaggcct ggaggcccat gtgctctgtg
      121 tctgggcatt gagtgtacca gatgtcttat ctgggccatg caggccctgt gggaggcctc
      181 ctacatatat agggggcccc aacacagagg atggcacttg agcctgctgt ccatctggaa
      241 aacgttgcat gcttggcccg ctgctctgga ctgcctctct gcctgttgtg agttccagcc
      301 agccctgata gacccattcc ttttgtagag tatataaggt ctgccccagt agctcctagt
      361 gactctgggc ctggttggtg gcttagattc cacagaaggt gctcaattat ccacctgttc
      421 ctccttctgg gctcccttcc cccattgtct ggagcccagc ccagagcccc cctgtttcag
      481 gatttcctcc atatcctccc acttagaaca agatcaggag ggctgtggtc acttgatcct
      541 ggcaggacag caggagagtg ggtggtgggc aggtaaggga gcctatgact ggcttgtcta
      601 agccccaggt cagggagctg gcaccggagg gaggcgggga ctgaactctc taccaacctt
      661 ctctatccag cctgggcaga atggattcca gggctttaga gggggggaat gccacagtga
      721 gaagcccagg caggatgtcc cgtgggtgag gaacaggctg tgcatccctt agctatagtg
      781 agtgagctgg gggctccctc ctgggaccct ggaacccagt ttctttacct cctccctttg
      841 ctgtcagagc aaggagaaga agaggtaggt tccttggaat gtggcctgca aggtcaaggt
      901 ctgggaggca gctgccttta aggacttaaa actgagaggg gtgtgtgtgt gtgtgtgtgt
      961 gtgtgtgtgt gtgtgtgtgt gtacagctga ggagaagctg gggcagggac ttggccttgg
     1021 ccatctgcct cctgtttact tggcctttgc tgctgggact agggtggtcg catggccgcg
     1081 ggatatcact agtgcggccg cctgcaggtc gaccatatgg gagagctccc aac
//