From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Rattus norvegicus lipoprotein lipase mRNA, complete cds.
Date: 31 Dec 92 01:39:42 GMT
Distribution: bionet
Checksum: 21513 8

LOCUS       RATLLIPA     3617 bp ss-mRNA            ROD       17-DEC-1992
DEFINITION  Rattus norvegicus lipoprotein lipase mRNA, complete cds.
ACCESSION   L03294
KEYWORDS    lipoprotein lipase.
SOURCE      Rattus norvegicus (strain Sprague-Dawley) male 4-week-old
            testicular cDNA to mRNA.
  ORGANISM  Rattus norvegicus
            Eukaryota; Animalia; Chorqata; Vertebrata; Mammalia; Theria;
            Eutheria; Rodentia; Myomorpha; Muridae; Murinae.
REFERENCE   1  (sites)
  AUTHORS   Etienne,J. and Brault,D.
  TITLE     Les deficits en lipoproteine lipase
  JOURNAL   Ann. Biol. Clin. (Paris) 50, 299-309 (1992)
  STANDARD  full automatic
REFERENCE   2  (bases 1 to 3617)
  AUTHORS   Brault,D., Noe,L., Etienne,J., Hamelin,J., Raisonnier,A., Souli,A.,
            Chuat,J.C., Dugail,I., Quignard-Boulange,A., Lavau,M. and
            Galibert,F.
  TITLE     Sequence of rat lipoprotein lipase-encoding cDNA
  JOURNAL   Gene (1992) In press
  STANDARD  full automatic
COMMENT     In [Gene 121, 237-246 (1992)] (Fig 2 and 3) the rat sequence for
            lipoprotein lipase (LPL) has been compared to that of mouse, human,
            guinea pig, bovine and chicken. The major divergences between
            human, mouse and rat LPLs were observed in the untraslated exon 10,
            where:
              (i) The rat cDNA exhibits a 157-bp insertion and a
                   81-bp deletion relative to human.  The mouse also
                   has a deletion and an insertion (some homology
                   with the rat insertion).
             (ii) Neither the B1 repeat nor the homopurine stretch
                   reported in the mouse can be recognized in the rat.
            (iii) The rat cDNA displays several A+T-rich stretches.
FEATURES             Location/Qualifiers
     sig_peptide     175..255
                     /codon_start=1
     mat_peptide     256..1596
                     /EC_number="3.1.1.34"
                     /codon_start=1
     misc_binding    1129..1155
                     /bound_moiety="heparin"
                     /note="putative"
     misc_feature    2402..2558
                     /note="some homology with 157-bp insertion in mouse;
                     157-bp insertion relative to human sequence"
     polyA_signal    3266..3271
                     /note="putative"
     misc_feature    3329^3330
                     /note="71-bp deletion in mouse relative to human sequence;
                     81-bp deletion relative to human sequence"
     polyA_signal    3584..3589
                     /note="putative"
     CDS             175..1599
                     /product="prelipoprotein lipase"
                     /codon_start=1
                     /translation="MESKALLLVALGVWLQSLTAFRGGVAAADGGRDFSDIESKFALR
                     TPEDTAEDTCHLIPGLADSVSNCHFNHSSKTFVVIHGWTVTGMYESWVPKLVAALYKR
                     EPDSNVIVVDWLYRAQQHYPVSAGYTKLVGNDVARFINWLEEEFNYPLDNVHLLGYSL
                     GAHAAGVAGSLTNKKVNRITGLDPAGPNFEYAEAPSRLSPDDADFVDVLHTFTRGSPG
                     RSIGIQKPVGHVDIYPNGGTFQPGCNIGEAIRVIAEKGLGDVDQLVKCSHERSIHLFI
                     DSLLNEENPSKAYRCNSKEAFEKGLCLSCRKNRCNNVGYEINKVRAKRSSKMYLKTRS
                     QMPYKVFHYQVKIHFSGTENDKQNNQAFEISLYGTVAESENIPFTLPEVATNKTYSFL
                     IYTEVDIGELLMMKLKWKNDSYFRWSDWWSSPSFVIEKIRVKAGETQKKVIFCAREKV
                     SHLQKGKDAAVFVKCHDKSLKKSG"
BASE COUNT     1049 a    780 c    812 g    976 t
ORIGIN      
        1 ttctgcccct tgtagctgtt ctgccctccc ctttaaaggt tgacttgccc cgcggcgctc
       61 caccgcgctc tagtcctctg acgcctccgg ctcaaccctt tgcaacgcgg atccccgccc
      121 gcctgactgc ccgcgcagcg cagttccagc agcaaagcag aagggcgcgc cgagatggag
      181 agcaaagccc tgctcctggt ggccctggga gtttggctcc agagtttgac cgccttccgc
      241 ggaggggtgg ccgcagcaga cgggggaaga gatttctcag acatcgaaag taaatttgcc
      301 ctaaggaccc ctgaagacac agctgaggac acttgtcatc tgattcctgg attagcagac
      361 tctgtgtcta actgccactt caaccacagc agcaaaacct ttgtggtgat ccatggatgg
      421 acggtgacag gaatgtatga gagttgggtg cccaaacttg tggctgccct atacaaaaga
      481 gaacctgact ccaatgtcat tgtagtagac tggttgtatc gggcccagca acattatcca
      541 gtgtctgccg gctataccaa gctggtggga aatgatgtgg ccaggttcat caactggctg
      601 gaggaagaat ttaactaccc cctagacaat gtccacctct tagggtacag tcttggagcc
      661 catgctgctg gcgtggcagg aagtctgacc aacaagaagg tcaatagaat tactggcttg
      721 gatccagctg ggcctaactt tgagtatgca gaagccccta gtcgcctttc tcctgatgat
      781 gcggatttcg tagatgtctt acacacattt accagggggt cgcctggtcg aagtattggg
      841 atccagaaac cagtagggca tgttgatatt tatcccaatg gaggcacttt ccagccagga
      901 tgcaacattg gagaagccat tcgtgtaatt gcagagaagg ggcttggaga tgtggaccag
      961 ctggtgaagt gctcgcacga gcgctccatc catctcttca ttgactccct gctgaatgaa
     1021 gaaaacccca gcaaggcata caggtgcaat tccaaggagg cctttgagaa agggctctgc
     1081 ctgagttgca gaaagaatcg ctgtaacaac gtgggctatg agatcaacaa ggtcagagcc
     1141 aagagaagca gtaagatgta cctgaagact cgctctcaga tgccctacaa agtattccat
     1201 taccaagtca agattcactt ttctggaact gagaatgaca agcaaaacaa ccaggccttc
     1261 gagatttctc tgtatggcac agtggctgaa agtgagaaca ttcccttcac cctgccggag
     1321 gtcgccacaa ataaaaccta ctccttcttg atttacacgg aggtggacat cggggaattg
     1381 ctgatgatga agcttaagtg gaagaacgac tcctacttcc gctggtcaga ctggtggagc
     1441 agtcccagct ttgtcatcga gaagatccga gtgaaagccg gagagactca gaaaaaggtc
     1501 atcttctgtg ccagggagaa agtttctcat ctgcagaaag gaaaggacgc tgcagtgttt
     1561 gtgaaatgcc atgacaagtc tctgaagaag tcgggctgac actggacaaa ccaacaagag
     1621 aagaaagcat ctgagttctt tgaagaccga agaaaatgaa gtaaatttta tttaaaaaaa
     1681 atacccttgt ttgggtgttt gaaagtggat tttcctgagt attaatccca gctatatctt
     1741 gttagttaaa tagaagacag tgtcaaatat taaaaggtgg ctaacacaac gtgaggaacc
     1801 taatggccga tagcatgtcc tccagcatca gaagacagca gagaggagaa gcatgccatc
     1861 ttatatccct taagaaggaa tcatttgttc ccaaccatac aagactcctt catgtgaccc
     1921 atttggtcat ggtctaaaat tagtaagggc ctcttatttt cattagatct ctgaggtttt
     1981 aaattgagac cttctcaaag ttctcttgaa gtctaatata gacaacattt ttttgtgctg
     2041 tgagtcagat ccatttcttt agcagttgaa acagctggcc attgtaacta gttcttttac
     2101 catcaggata tagcacccct accaaataaa ataaataaat aaagtgacca gggacatgtg
     2161 actttgcaaa agcaatggac gacgtggctc gtggattcct gacccttagt cccaccacaa
     2221 cgaagtacaa gtcagtagag gtacaaaacc tagactgagt aattcttagt agacttcaag
     2281 ttttatggct taattcctct gtcttttaaa aacgtgtcac atattataac attattctct
     2341 agacagatgt tgaaatgagc ttgtgattca ggtgacatat gaattgagct gagagaaaat
     2401 aatgccctgg ctgattttat ttctctgttt tgctttcttg agaaaaggaa tacttgtccc
     2461 actccgtatc tgagcctgac caagaactaa actatgtact tcaggcttac cttgaactct
     2521 caaccatcct gccttggctt cctgagtgct gggagcttga taaccataat tttattatca
     2581 gatttttctt agtcattttc accaatagaa cacattcaat gcccaatcgt tagcatttcg
     2641 tttgagactc atcttgaccg tacctctgtc acacgtctaa cacatcacat taatttctag
     2701 tttagaagtg atcaagttca aattctgcac tgcgcaaagt acaagtttta gagcaggacc
     2761 attttttttt taccacgtaa aagtcgaaat tactaggaaa tgtgtatatc gatgcttgta
     2821 cactgttgcg tgcaaagtga ggagccttct attgtgatag ccatagacag taccaggctc
     2881 gttgccgctc ttttgtttta ctataaaaaa ataatgaaga attatttatg aacaagatct
     2941 catatgttca gattgctttt actattcatc aatataaaat gttaaaaaaa aaaaataaaa
     3001 caagttctat ctcagaggct gttgctggga acagaaactg tgaaatgtgt gggtatctga
     3061 acacctacac acaagcaaag ccccacaaga gtctttgtca ttcaatgtca ttcagaaagg
     3121 aaagagtcaa gagatatacc ataatatgtc agagaagtag ttccagatat gctggaatgc
     3181 tagcccttgc taggagaaag ttggttgtgc ctatgtaata taggacaaaa gtgactggtt
     3241 tcattaggct cagtgtcatt ctaacaataa aatgatgtac catatgtcac cagcatcccc
     3301 attattgcta attcatggca gtatatacgt acatatctca atgatgcttt gactttaaat
     3361 tttatttatt agctgtaaat aatgtgtggg tgtgtaagga agcttgtaaa cactggaaac
     3421 gctgttgtgg ctatctgggg tgtagatttg tggtgctaac tctgtgtcca cctccatcag
     3481 tgattgtctc actgagccaa ctcactctga tgaacagcac aatggaatag cttttgaagg
     3541 aagaaaataa actcacctgt gtgaagaaat gggatctgct ttcaataaaa tcgagaacgt
     3601 tttatccgga atccgcg
//