From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Saccharomyces cerevisiae 1,4-glucan-6-(1,4-glucano)-transferase
Date: 31 Jul 92 10:02:08 GMT
Distribution: bionet
Checksum: 43130     6

LOCUS       YSCGBEAA     2690 bp ds-DNA             PLN       31-JUL-1992
DEFINITION  Saccharomyces cerevisiae 1,4-glucan-6-(1,4-glucano)-transferase
            (GLC3) gene, complete cds.
ACCESSION   M76739
KEYWORDS    1,4-glucan-6-(1,4-glucano)-transferase; glycogen branching enzyme.
SOURCE      Saccharomyces cerevisiae (strain GRF88) MATa DNA.
  ORGANISM  Saccharomyces cerevisiae
            Eukaryota; Fungi; Eumycota; Hemiascomycetes; Endomycetales;
            Saccharomycetaceae.
REFERENCE   1  (bases 1 to 2690)
  AUTHORS   Thon,V.J., Vigneron-Lesens,C., Marianne-Pepin,T., Montreuil,J.,
            Decq,A., Rachez,C., Ball,S.G. and Cannon,J.F.
  TITLE     Coordiante regulation of glycogen metabolism in the yeast
            Saccharomyces cerevisiae: induction of glycogen branching enzyme
  JOURNAL   J. Biol. Chem. 267, 15224-15228 (1992)
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     CDS             226..2340
                     /EC_number="2.4.1.18"
                     /standard_name="glycogen branching enzyme"
                     /product="1,4-glucan-6-(1,4-glucano)-transferase"
                     /gene="GLC3"
                     /evidence=EXPERIMENTAL
                     /codon_start=1
                     /translation="MYNIPDNVKGAVEFDPWLKPFADVLSERRYLADKWLYDITHATP
                     DGSYQSLSKFARDSYKSYGLHANPETKEITYKEWAPNAERAFLVGDFNNWDTTSHELK
                     NKDEFGNFTITLHPLPNGDFAIPHDSKIKVMFILPDGSKIFRLPAWITRATQPSKETS
                     KQFGPAYEGRFWNPENPYKFVHPRPKFSESVDSLRIYEAHVGISSPEPKITTYKEFTE
                     KVLPRIKYLGYDAIQLMAIMEHAYYASFGYQVTNFFAASSRFGTPEELKELIDTAHSM
                     GILVLLDVVHSHASKNVEDGLNMFDGSDHQYFHSISSGRGEHPLWDSRLFNYGKFEVQ
                     RFLLANLAFYVDVYQFDGFRFDGVTSMLYVHHGVGAGGSFSGDYNEYLSRDRSFVDHE
                     ALAYLMLANDLVHEMLPNLAVTVAEDVSGYPTLCLPRSIGGTGFDYRLAMALPDMWIK
                     LIKEKKDDEWEMGSIVYTLTNRRYGEKVVAYCESHDQALVGDKTLAFWLMDAAMYTDM
                     TVLKEPSIVIDRGIALHKMIRLITHSLGGEAYLNFEGNEFGHPEWLDFPNVNNGDTYK
                     YARRQFNLADDPLLRYQNLNEFDRSMQLCEKRHKWLNTKQAYVSLKHEGDKMIVFERN
                     NLLFIFNFHPTNSYSDYRVGVEKAGTYHIVLNSDRAEFGGHNRINESSEFFTTDLEWN
                     NRKNFLQVYIPSRVALVLALKE"
BASE COUNT      789 a    497 c    586 g    818 t
ORIGIN
        1 aagctttgtc caaggggctg ttgcaacggt aaaggtagtc gagccaagtg acaccagcaa
       61 acaaggaaac aaggaagcaa ggaaggcttt ccattttcca ataacgacat gagtctatct
      121 atggtgtata aaagtggcag gcttttccta catttttttt ccctgataac ttcctgttac
      181 tatttaagaa caccaaacca agtataaaga accgtcaaga ataaaatgta caacattcca
      241 gataatgtca aaggggctgt agaatttgat ccatggttga aaccgtttgc cgatgtactt
      301 tctgaaagaa gatacctagc tgacaagtgg ttgtacgaca taactcatgc tacgcctgat
      361 ggttcgtatc agtcactcag taagtttgct agagattcat acaagtcata tgggttgcat
      421 gccaatccgg aaaccaaaga aatcacttac aaagagtggg cacccaatgc ggaacgtgca
      481 tttctagtgg gagatttcaa taactgggat accacatctc atgagctcaa gaacaaggac
      541 gaatttggaa attttacaat cacacttcat cctttaccta acggcgattt cgccatccct
      601 catgattcga agattaaagt catgtttatt ttgccagatg gttccaagat ctttcgttta
      661 cctgcgtgga tcacaagggc tacacagcct tctaaggaaa cttctaaaca gttcgggccc
      721 gcatatgaag gtaggttttg gaatccagag aacccctaca aatttgtaca tccaaggcca
      781 aagtttagtg aatctgtaga ttccttaagg atttatgaag cccacgtcgg tatttcgagt
      841 ccagaaccaa agataaccac atataaagaa tttactgaga aggtcttacc cagaatcaaa
      901 tatcttggct acgatgcaat tcaattaatg gctattatgg aacatgctta ttatgcatca
      961 tttggctacc aagtgaccaa tttttttgcg gcgagctccc gtttcggcac ccctgaggaa
     1021 ttaaaggagc tgattgatac tgcacattct atgggcatcc tagttttatt ggatgtagtt
     1081 catagccatg cttctaaaaa cgtcgaggat gggttgaaca tgtttgacgg ttctgatcat
     1141 cagtatttcc attctataag ctccggtagg ggtgagcatc cgctgtggga ttcccgtctt
     1201 tttaattacg gaaaatttga agtgcagcga ttcttattag caaacctggc attttatgtt
     1261 gacgtttatc agtttgatgg gtttagattc gatggtgtca catcaatgtt atacgttcat
     1321 catggtgttg gtgccggtgg gtcttttagc ggtgactata acgaatatct ttctcgtgac
     1381 aggtccttcg tcgatcatga agccttagct tatttaatgt tagccaatga tttggttcac
     1441 gaaatgttgc caaatctggc tgtaactgtt gcagaagatg tctctggtta tccaacttta
     1501 tgtttacccc gctccatagg tggtactggt ttcgactaca gattagcaat ggctttgcct
     1561 gatatgtgga tcaaactaat caaggagaag aaagatgatg aatgggagat gggaagtatt
     1621 gtatacactt taactaatag acgatatggt gaaaaagtgg ttgcttattg tgagtcgcac
     1681 gaccaagctt tagttggtga taagacatta gcgttttggt tgatggatgc cgctatgtac
     1741 accgatatga ctgtattgaa ggaaccatcc atcgttattg accgtggtat tgctctacac
     1801 aagatgatta ggctgatcac tcactcgctg ggaggcgagg cttatttgaa cttcgaaggt
     1861 aatgagttcg gtcatccaga atggttggat ttccctaatg ttaacaatgg cgatacgtac
     1921 aagtatgctc gtaggcagtt taatttggct gatgatccct tactgcgtta ccagaattta
     1981 aatgagtttg atagatcaat gcaattatgt gagaaaaggc ataagtggct gaacacgaaa
     2041 caggcttatg tttcattaaa acatgaaggt gacaaaatga ttgtgtttga aagaaataat
     2101 ctgttgttta ttttcaactt ccatcctaca aacagctaca gcgattatag agttggtgtc
     2161 gaaaaagctg gtacttatca catcgtgttg aactcagatc gtgctgaatt tggtggacat
     2221 aacagaatca acgaaagttc cgaattcttt accaccgatt tggaatggaa caacaggaag
     2281 aacttcctcc aagtttatat tcctagtaga gtcgctttgg tgcttgcctt aaaagaatga
     2341 actctatact atattgctta ctgaaaatca agactcaata atttttttct ctttttcgga
     2401 gaaaaaaaga aagccctgta ggggcctcga cccctaacct tatgattaag agtcatacgc
     2461 gctaccgatt gcgccaacaa ggcttttttt ccttgggaaa atctcttcca aggtttaacc
     2521 tatactgtct gtgtatgtct aaaacgaagc attggttgtt cattgattgc aacagtaatg
     2581 aataattagg gttattgaag agaatgtgga ttttgatgta attgttggga ttccattgtg
     2641 attaaggcta taatattagg tatgtagata tactagaagt tctcctcgag
//