From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Mycobacterium fortuitum DNA sequence, ORFs 1 and 2.
Date: 12 Sep 92 10:02:55 GMT
Distribution: bionet
Checksum: 31819     9

LOCUS       MSGORF       4821 bp ds-DNA             BCT       12-SEP-1992
DEFINITION  Mycobacterium fortuitum DNA sequence, ORFs 1 and 2.
ACCESSION   M60875
KEYWORDS    .
SOURCE      Mycobacterium fortuitum DNA.
  ORGANISM  Mycobacterium fortuitum
            Prokaryotae; Firmicutes; Mycobacteria; Mycobacteriaceae.
REFERENCE   1  (bases 1 to 4821)
  AUTHORS   Labidi,A., Mardis,E., Roe,B.A. and Wallace,R.J.Jr.
  TITLE     Cloning and DNA sequence of the Mycobacterium fortuitum biovarvar
            fortuitum plasmid pAL5000
  JOURNAL   Plasmid 27, 130-140 (1992)
  STANDARD  full staff_entry
FEATURES             Location/Qualifiers
     -35_signal      85..87
                     /note="-35 signal for ORF1"
     -10_signal      121..126
                     /note="-10 signal for ORF1"
     RBS             181..183
                     /note="RBS for ORF1"
     CDS             188..721
                     /note="ORF1"
                     /codon_start=1
                     /translation="MTKNPGHIAWETEWLHSDLYTLSHIEAELGANMPPPRWRQQTTY
                     KAAPTPLGRNCALFDSVRLWAYRPALMRIYLPTRNVDGLGRAIYAECHARNAEFPCND
                     VCPGPLPDSEVRAIANSIWRWITTKSRIWADGIVVYEATLSARQSAISRKGAAARTAA
                     STVARRAKSASAMEALL"
     -35_signal      2063..2067
                     /note="-35 signal for ORF2"
     -10_signal      2089..2092
                     /note="-10 signal for ORF2"
     RBS             2171..2174
                     /note="RBS for ORF2"
     CDS             2176..4026
                     /note="ORF2"
                     /codon_start=1
                     /translation="MPSNRCPRLRRSGHSDQHNNQRRHSDDEPRSDDPNHCAVHTGGH
                     PSNSPASMRVCKDCHGALLGPVSTHCSKKSTAASAPNSLCCSACSSALHVFTSGADNS
                     TTLLAIDALVAAAMPATSQSPGSRSARHNSSAIRPRSSACLTTFRRRGAHPGTEPDRH
                     YRNGYARRQRRRWRRHVHHHRTGNQRRFIDGRLNRPAFVGQQVRELVGIDVLPAGGSS
                     SRRMWDTSALSRASSPVRIRSEARSRRGCGMSGRAAPPAPPIGPPIGIPPMPPIIPVE
                     PAGPVFNGGRPADGDVEAVRPEIWAGSTRPPVTVGLAAGVVGATTPPTTPRPAIAEPR
                     GGGCVRPARIVPASRLLLNTAEPAASRESAGHHGRAGGHRSGCTCRGWWRRQRRRLHH
                     RPWAADTSVVAPPPPTIVSSAPPPTMVSSQPSRGWRSRGDRKMPLSWPDTLESVSGSS
                     GRPSVADVHARSNRSSAAWTSGSAAVPPRMTGRPRPASPTARRAVGDFQQVAAHFRHL
                     FLGGRSPHRTQCRPETARPRSTPATRPCRCAPETRPRWSANRRHNGWRPGRRAISSRE
                     RYRSWSCVIPSFELWIKRRYTLGQASWIRRQANSALKRARPCGGGTPQGL"
BASE COUNT      860 a   1601 c   1526 g    834 t
ORIGIN
        1 gggggtccca tccgctgccc aacgcgatcg tgggcaatcg cgccaacggc cacgcacacg
       61 cagtgtgggc actcaacgcc cctgttccac gcaccgaata cgcgcggcgt aagccgctcg
      121 catacatggc ggcgtgcgcc gaaggccttc ggcggccgtc gacggcgacc gcagttactc
      181 aggcctcatg accaaaaacc ccggccacat cgcctgggaa acggaatggc tccactcaga
      241 tctctacaca ctcagccaca tcgaggccga gctcggcgcg aacatgccac cgccgcgctg
      301 gcgtcagcag accacgtaca aagcggctcc gacgccgcta gggcggaatt gcgcactgtt
      361 cgattccgtc aggttgtggg cctatcgtcc cgccctcatg cggatctacc tgccgacccg
      421 gaacgtggac ggactcggcc gcgcgatcta tgccgagtgc cacgcgcgaa acgccgaatt
      481 cccgtgcaac gacgtgtgtc ccggaccgct accggacagc gaggtccgcg ccatcgccaa
      541 cagcatttgg cgttggatca caaccaagtc gcgcatttgg gcggacggga tcgtggtcta
      601 cgaggccaca ctcagtgcgc gccagtcggc catctcgcgg aagggcgcag cagcgcgcac
      661 ggcggcgagc acagttgcgc ggcgcgcaaa gtccgcgtca gccatggagg cattgctatg
      721 agcgacggct acagcgacgg ctacagcgac ggctacaacc ggcagccgac tgtccgcaaa
      781 aagccgtgac gcgccgaagg cgctcgaatc accggactat ccgaacgcca cgtcgtccgg
      841 ctcgtggcgc aggaacgcag cgagtggctc gccgagcagg ctgcacgcgc gcgaagcatc
      901 cgcgcctatc acgacgacga gggccactct tggccgcaaa cggccaaaca tttcgggctg
      961 catctggaca ccgttaagcg actcggctat cgggcgagga aagagcgtgc ggcagaacag
     1021 gaagcggctc aaaaggccca caacgaagcc gacaatccac cgctgttcta acgcaattgg
     1081 ggacgggtgt cgcgggggtt ccgtgggggg ttccgttgca acgggtcgga caggtaaaag
     1141 tcctggtaga cgctagtttt ctggtttggg ccatgcctgt ctcgttgcgt gtttcgttgc
     1201 gccgttttga ataccagcca gacgagacgg ggttctacga atcttggtcg ataccaagcc
     1261 atttccgctg aatatcgggg agctcaccgc cagaatcggt ggttgtggtg atgtacgtgg
     1321 cgaactccgt tgtagtgcct gtggtggcat ccgtggccac tctcgttgca cggttcgttg
     1381 tgccgttaca ggccccgttg acagctcacc gaacgtagtt aaaacatgct ggtcaaacta
     1441 ggtttaccaa cgatacgagt cagctcatct agggccagtt ctaggcgttg ttcgttgcgc
     1501 ggttcgttgc gcatgtttcg tgtggttgct agatggctcc gcaaccacac gcttcgaggt
     1561 tgagtgcttc cagcacgggc gcgatccaga agaacttcgt cgtgcgactg tcctcgttaa
     1621 cgctcgaagc gatgccgatc aaatcggctg tgccgtcgcg gttcttgcgg tacagcggcg
     1681 cgcctgagtc gccatgtacg gtgcgcagct gagcggtgat cgtgtacttg gcagccacaa
     1741 cgaccggccc acaggtctcg ccggatttca tcccgtactt gcagacctcg gtgccgtacg
     1801 gaacatcttt gcggtccttc agcaccgcac gcacggggta tcttccgccg attttgaacg
     1861 aagcgatcgg tgttcccgga gcaaacgcaa tcaccccagc gtcggtgtag accggcaata
     1921 gatcatcgtc gccggtaatc gtggtgcctg gtcctctcga cgcaacgtag gtacccaacg
     1981 tccgctgtgt atccggtttc tctgcaaccg aatacagcac cgggcccccg tggtcgcagt
     2041 gccccgcagc gatgccgtac agcttgtggg tcgacgggtc acctgcgatc acaccgagcg
     2101 tgcaggtagc gaagtcctca tcaccaccag gacgggcctg ggcgatacca gcgccggggg
     2161 cgatcccgcc aggaaatgcc gtccaatcgg tgtccgcgac tgcggcggag cggacactcc
     2221 gaccaacaca acaaccaacg tcgtcatagc gacgacgaac cacgatcgga tgatccgaat
     2281 cactgcgctg tccatacagg cggccacccc tcgaactcac cagcttcaat gcgcgtctgc
     2341 aaagactgcc atggagcgct actcgggccg gtctcaacgc actgctcgaa gaaatcgaca
     2401 gcggccagtg caccgaactc cttgtgctgc tcggcttgca gctcggcgct ccacgtcttc
     2461 acctcgggcg cggacaattc gacgaccttg ttagcgatcg acgcattggt cgccgcagca
     2521 atgcccgcca catcccagtc ccctggatcg aggtcggcgc ggcacaacag ctccgcgatc
     2581 cgaccccgat ccagcgcctg cctcaccact tttcgtcgtc gcggggctca cccgggtact
     2641 gaaccggatc gccactatcg aaacggctac gcgcggcggc agcggcggcg ctggcggcgg
     2701 cacgttcatc accaccggac cgggaaccag cgtcgattca tcgatggccg gctgaatcgg
     2761 ccggcgttcg tcgggcagca ggtccgcgag ctcgtcggca tcgatgtact gccggccggc
     2821 ggatcgtcgt cacgcagaat gtgggacacc agcgccttgt cgcgggcctc ttcgccggtg
     2881 aggatccgct cggaggcgcg gtcgcggcgc ggctgtggca tgtcggggcg tgccgctccc
     2941 ccggcgccgc ccatcggccc gcccattggc attccgccca tgccgcccat cattcctgtg
     3001 gagccagctg gcccggtctt caatggaggc aggcccgctg acggcgacgt ggaggcggtg
     3061 cgccccgaaa tctgggccgg atcaactcgg ccaccggtca cggtcggatt ggcggccggt
     3121 gttgtcggtg cgacaacacc gccgacaacg ccgcgccccg ccatcgccga accacggggt
     3181 ggtgggtgcg tccgacctgc cagaatcgtc ccggcgtcgc ggctgctgct gaacaccgcc
     3241 gagcccgccg ccagtcggga aagcgctggg catcatggtc gggccggggg ccatcggagc
     3301 gggtgcacct gtcggggctg gtggcggcgt cagcgccgtc gcctgcacca tcggccgtgg
     3361 gccgccgaca cctccgtggt cgcaccgccg ccgccgacga tcgtgtcgtc agcgccgccg
     3421 ccgacgatgg tgtcgtccca accgtcgcgc ggctggaggt cgcggggcga ccggaaaatg
     3481 cctttatcgt ggccggacac cttggaatcg gtgtccggct cgtcgggcag gccttccgtc
     3541 gctgacgtgc acgcgcgctc caatcgctcc agcgccgcct ggacctcggg atcggcagcc
     3601 gtcccgcccc gaatgaccgg gcggccgcgg ccggcctctc ccaccgcacg cagggccgtc
     3661 ggcgattttc agcaggtcgc cgcccatttc cgacatcttt tcctcggcgg ccgatcgccg
     3721 caccggaccc aatgtcgtcc ggaaacggct cggccgcgat cgactccagc aacgcggcca
     3781 tgtcgatgcg ctcctgaaac tcggcctcgt tggtcagcga atcgccgtca taacggatgg
     3841 cgcccgggcc gccgcgcgat atcgagccga gaacgttatc gaagttggtc atgtgtaatc
     3901 ccctcgtttg aactttggat taagcgtaga tacacccttg gacaagccag ttggattcgg
     3961 agacaagcaa attcagcctt aaaaagggcg aggccctgcg gtggtggaac accgcagggc
     4021 ctctaaccgc tcgacgcgct gcaccaacca gcccgcgaac ggctggcagc cagcgtaagg
     4081 cgcggctcat cgggcggcgt tcgccacgat gtcctgcact tcgagccaag cctcgaacac
     4141 ctgctggtgt gcacgactca cccggttgtt gacaccgcgc gcggccgtgc gggctcggtg
     4201 gggcggctct gtcgcccttg ccagcgtgag tagcgcgtac ctcacctcgc ccaacaggtc
     4261 gcacacagcc gattcgtacg ccataaagcc aggtgagccc accagctccg taagttcggg
     4321 cgctgtgtgg ctcgtacccg cgcattcagg cggcaggggg tctaacgggt ctaaggcggc
     4381 gtgtacgcgg ccacagcggc tctcagcggc ccggaaacgt cctcgaaacg acgcatgtgt
     4441 tcctcctggt tggtacaggt ggttgggggt gctcggctgt cgcggttgtt ccaccaccag
     4501 ggctcgacgg gagagcgggg gagtgtgcag ttgtggggtg gcccctcagc gaaatatctg
     4561 acttggagct cgtgtcggac catacaccgg tgattaatcg tggtctacta ccaagcgtga
     4621 gccacgtcgc cgacgaattt gagcagctct ggctgccgta ctggccgctg gcaagcgacg
     4681 atctgctcga ggggatctac cgccaaagcc gcgcgtcggc cctaggccgc cggtacatcg
     4741 aggcgaaccc aacagcgctg gcaaacctgc tggtcgtgga cgtagaccat ccagacgcag
     4801 cgctccgagc gctcagcgcc c
//