From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Rhizobium meliloti ORF1, complete cds; and phosphomannose isomerase
Date: 28 Jan 93 05:48:14 GMT
Distribution: bionet
Checksum: 47579 5

LOCUS       RHMPMIA      2022 bp ds-DNA             BCT       27-JAN-1993
DEFINITION  Rhizobium meliloti ORF1, complete cds; and phosphomannose isomerase
            (PMI) gene, complete cds.
ACCESSION   M96584
KEYWORDS    phosphomannose isomerase.
SOURCE      Rhizobium meliloti (strain 2011) DNA.
  ORGANISM  Rhizobium meliloti
            Prokaryotae; Gracilicutes; Scotobacteria; Rhizobiaceae.
REFERENCE   1  (bases 1 to 2022)
  AUTHORS   Schmidt,M.G., Arnold,W., Niemann,A., Kleickmann,A. and Puehler,A.
  TITLE     The Rhizobium meliloti pmi gene encodes a new type of
            phosphomannose isomerase
  JOURNAL   Gene 122, 35-43 (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     CDS             214..708
                     /note="putative;  ORF1"
                     /codon_start=1
                     /translation="MNIDNDLRRIALQEQQLQFERFDLDTAWKLGATLRRMAGERKLG
                     CVIDITLYSMQVFYAALDGATPDNPNWVRRKRNTVFRLFKSTYATGLSLLKQQTNLQA
                     KLGLPDAEFAAHGGSFPIVVKGTGCIGAVTVSGLPQREDHNLVVEALAELLGADHDAL
                     KLES"
     CDS             719..1882
                     /EC_number="5.3.1.8."
                     /gene="PMI"
                     /evidence="EXPERIMENTAL"
                     /partial
                     /product="phosphomannose isomerase"
                     /codon_start=1
                     /translation="VGMDIHLQAGELAGWLNDAALPLWRQKGFDGEGGGFVETIDMKG
                     EPTRDDRRSRVQPRQVYCFAAAGRRGWDGDWRTAAEGGLLYFDRVYGQPGGFYGALAN
                     ADGKLIDASFDLYNQAFALLAFAHLAEVLPERGAEMVGRSDKLRRQLEARCKHPLAGF
                     EEDDPPRLPLGSNPHMHLFEACLASEEVEGFDRVAWANLADEIAHLAMDRFIDAESGA
                     LREFFDHDWAPFPGEKGRIVEPGHLFEWAWLLLRWAERRGNAQAIVKARRLFEIGEKD
                     GTCPDRDVVVMTLFDDFSVADPTARLWPQTEWLKAAIRFAALTEGAERERYLASAGRA
                     AAALQRFLNVPVRGLWRDKQKADGSFVEEPAPASTFYHILCAIYELEDCLKRM"
BASE COUNT      359 a    618 c    676 g    369 t
ORIGIN      
        1 gtcgacgaga tttacggcga gttgccggcg tcgatcacca gatatctctt cggcgacagt
       61 ttcgagacgc cgattcgcaa ggcggtgcac ggcgacagtt ccggcgtgcg cggcgccgca
      121 tggctctgga aggattaacc ggccaggcgg ggaattttgc cggatcgcgc atcgcactcg
      181 tccagttcga accactgacc aagcggaggc atcatgaaca tagacaatga tctgcgccgg
      241 attgcgctgc aggagcagca attgcagttc gagcgcttcg atctcgatac ggcctggaag
      301 ctaggcgcca cgcttcgccg gatggccggg gaacgcaagc tcggctgcgt catcgacatc
      361 acgctctact ccatgcaggt cttctatgcg gccctcgatg gggcgacgcc ggacaatccg
      421 aactgggtgc gccgcaagcg caacacggtg ttccgcctgt tcaagagcac gtacgccacg
      481 gggctcagcc tgctcaagca gcaaacgaat ttgcaggcga agctcggcct gccggatgct
      541 gagtttgccg cccacggcgg cagcttcccg atcgtcgtca agggaacggg ctgcatcggc
      601 gcggtcacgg tctccggcct gccgcagcgg gaggaccaca atctcgtggt cgaggcgctc
      661 gcagaacttc tcggcgcgga tcacgacgcg ctgaagctgg aaagctgacg gaaagcgggt
      721 gggcatggat attcatctcc aggcaggcga actcgcgggc tggctgaacg atgctgcctt
      781 gccgctctgg cggcagaaag gcttcgacgg ggagggtggc ggcttcgtcg agacgatcga
      841 catgaaaggc gaaccgacgc gcgacgatcg ccgctcccgc gtgcagcccc ggcaggtcta
      901 ctgcttcgcc gcggcgggcc ggcgcggctg ggatggagac tggcgcaccg ccgccgaagg
      961 cggccttctc tatttcgatc gcgtctacgg acagccgggc ggcttctacg gggcgctggc
     1021 gaatgccgac ggcaagctca tcgacgcttc cttcgacctc tacaaccagg ccttcgcctt
     1081 gcttgccttc gcccatttgg cggaagtcct gccggagcgc ggagccgaga tggtggggcg
     1141 cagcgacaaa ctcaggcggc agctcgaggc ccgttgcaag catccgctcg ccggtttcga
     1201 ggaggacgac ccgccgcgcc tgccgctcgg ctccaatccg catatgcacc ttttcgaggc
     1261 ctgtcttgcg agcgaggagg tggaaggctt cgaccgcgtc gcctgggcca atctcgccga
     1321 cgagatcgcg catctggcca tggaccggtt catcgacgcc gagagcgggg cgctgcgtga
     1381 gttcttcgat cacgactggg cgccgttccc cggcgaaaag ggccgtatcg tggagccggg
     1441 gcaccttttc gaatgggcgt ggctcctcct gcgctgggcg gaacggcgcg gcaatgcgca
     1501 ggcgatcgtc aaggcgcggc ggcttttcga gatcggcgag aaagacggaa cctgccccga
     1561 ccgggacgtg gtcgtcatga cgctcttcga cgatttctcc gttgccgatc cgacagcgcg
     1621 gctttggcca cagacggagt ggctgaaggc ggcgatccgc tttgcggctc tgacggaagg
     1681 tgcggagcga gagcgttatc tggcttcggc tgggcgcgcc gcagcggcgc tgcagcgctt
     1741 cctgaacgtg cctgtccgcg gcctctggcg cgacaagcag aaggcggatg gcagcttcgt
     1801 cgaagagccg gcgccagcaa gcaccttcta tcatattctt tgcgcgatct atgaactgga
     1861 ggattgcctt aagcggatgt gaccggcgtc ggcagacctt ccgtcctcat cgagagagcg
     1921 cattattcgg cccgccatcc gcggcgggct attttattgc caatctgcaa ttgtgaccgg
     1981 tgcgtatgtt gacataaaga tatcttatgt gagtaaatcg at
//