From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: D.pseudoobscura Adh gene for alcohol dehydrogenase
Date: 28 Dec 92 21:27:47 GMT
Distribution: bionet
Checksum: 45027 8

LOCUS       DROADHGN64   3487 bp ds-DNA             INV       01-DEC-1992
DEFINITION  D.pseudoobscura Adh gene for alcohol dehydrogenase
ACCESSION   X64473
KEYWORDS    ADH gene; alcohol dehydrogenase.
SOURCE      Drosophila pseudoobscura DNA.
  ORGANISM  Drosophila pseudoobscura
            Eukaryota; Animalia; Metazoa; Arthropoda; Uniramia; Insecta;
            Pterygota; Neoptera; Holometabola; Diptera; Brachycera;
            Cyclorrhapha; Schizophora; Drosophiloidea; Drosophilidae.
REFERENCE   1  (bases 1 to 3487)
  AUTHORS   Schaeffer,S.W. and Miller,E.L.
  TITLE     Estimates of gene flow in Drosophila pseudoobscura determined from
            nucleotide sequence analysis of the alcohol dehydrogenase region
  JOURNAL   Genetics 132, 471-480 (1992)
  STANDARD  full automatic
COMMENT     *source: strain=PS219; *source: isolate=Kiabab National Forest,
            Arizona; *source: chromosome=IV section 88;
               From EMBL    entry DPADHGN64;  dated 16-OCT-1992.
FEATURES             Location/Qualifiers
     mRNA            join(40..119,923..1028,1093..1497,1560..1975)
                     /note="adult mRNA (Adh)"
     mRNA            join(890..1028,1093..1497,1560..1975)
                     /note="larval mRNA (Adh)"
     promoter        8..14
                     /note="adult promoter (Adh)"
     prim_transcript 40..1975
                     /note="adult primary transcript (Adh)"
     exon            40..119
                     /note="adult exon 1a"
     intron          120..922
                     /note="adult intron"
     prim_transcript 890..1975
                     /note="larval primary transcript (Adh)"
     exon            890..1028
                     /note="larval exon 1"
     exon            923..1028
                     /note="adult exon 1b"
     intron          1029..1092
                     /number=1
     exon            1093..1497
                     /number=2
     intron          1498..1559
                     /number=2
     exon            1560..1975
                     /number=3
     polyA_signal    1952..1957
     polyA_site      1975
     CDS             join(936..1028,1093..1497,1560..1826)
                     /EC_number="1.1.1.1"
                     /gene="Adh"
                     /product="alcoholdehydrogenase"
                     /codon_start=1
                     /translation="MSLTNKNVVFVAGLGGIGLDTSRELVKRNLKNLVILDRIDNPAA
                     IAELKAINPKVTITFYPYDVTVPVAETTKLLKTIFAQVKTIDVLINGAGILDDHQIER
                     TIAVNYTGLVNTTTAILDFWDKRKGGPGGIICNIGSVTGFNAIYQVPVYSGSKAAVVN
                     FTSSLAKLAPITGVTAYTVNPGITKTTLVHKFNSWLDVEPRVAEKLLEHPTQTSQQCA
                     ENFVKAIELNKNGAIWKLDLGTLEPITWTQHWDSGI"
BASE COUNT      976 a    803 c    797 g    911 t
ORIGIN      
        1 cgtagtgtat ttaaggcact gcacatgtcg aggaccacga ttattgtcta cagtgcagtc
       61 agtagttgtc aggtgcagca ggcggaccag catttcttta aatctactca atcgatcaag
      121 taagtaactg taatccaagt acagtcggcc catcccactc ggtcactcgg ttctgccgta
      181 actcaagaaa tttcttgata gcgcagttcc agttgtaaaa cgtcgtttta aaaaacatct
      241 cgttggagct tgggttggaa aacgttcgag ttgggcgtag aaaagttcaa atataaaata
      301 acacaaaaga gaaaatatct tcccaaagca gctgtccatt ttttgttaaa gtgatagatc
      361 aaaaattcgt atgacgtatt tcccaagtgc tctttatgca aataatttcc tcaattctaa
      421 ttctacaaga agcacttttg gctcttgtcc agaaaaaaca atttgtttac acttgtcgga
      481 tgataacaga agcggaccga cggctaagta acataacagt cgcctgttaa gtaatgccct
      541 cactctctgt tagatggccg cgcgtgtcga ctccgataga ccagcagaca cgcagtagtt
      601 ttcctcaaga acctgaactc taaacataga cataatttac gataccttga gactcaaata
      661 aaaatacaag tatgagtggt attctattcc tgattagact cgcacacaca cacgtacgag
      721 tattaacata aatttgccgg caaataacca aaacaaaacg aacgcaaacg atccgaacac
      781 gtaatgcgag agataagaaa cagaaaagct cgacgtgaaa gaagcttctg gcggaagctc
      841 ttgccgaact ccctataaat acgagacaga aaaccagaat aaatataata acaaactgtt
      901 gaattgtccc atcgattccc agatcaaaag acagaatgtc tctcaccaac aagaacgtcg
      961 ttttcgtggc cggtctgggc ggcattggcc tggacaccag ccgggagttg gtcaagcgta
     1021 atctgaaggt aagagagaac gaattccatg gaatctatgg aatcctaaat ttcaaaaatt
     1081 catttatttt agaacctggt catcctggat cgcattgaca atccggctgc cattgccgaa
     1141 ctgaaggcaa tcaatcccaa ggtgaccatc accttctatc cctacgatgt gactgtgccc
     1201 gtcgctgaga ccaccaagct cctgaagacc atctttgccc aggtgaagac aatcgatgtc
     1261 ctgatcaacg gtgctggcat cctggacgat catcagatcg agcgcaccat tgccgttaac
     1321 tacacgggcc tggtcaacac caccacagcc attctggact tctgggacaa gcgcaagggc
     1381 ggcccaggcg gcatcatttg caacattggc tccgtcaccg gtttcaatgc catctaccag
     1441 gtgcccgttt actccggctc caaggcggcg gtggttaact ttacctcctc cctggcggta
     1501 agaaatccta caactatcct aactatccca aaactaaata tttctttaaa atcccataga
     1561 aactggctcc cattactggt gtcactgctt acactgtcaa tcctggcatc actaagacca
     1621 ctctggtcca caaattcaac tcgtggctgg atgtggagcc ccgtgtggcg gagaagctgc
     1681 tcgagcatcc cacccagacc tctcagcagt gcgccgagaa ctttgtgaag gccatcgagc
     1741 tgaacaagaa cggtgccatc tggaagttgg atctgggcac cttggagccc atcacatgga
     1801 cccagcactg ggactcgggc atctaaacca tcccagagac tctatgggac atggcgtttt
     1861 agcttttagt ttcttttttt ccactcaatt gttacgtata tacatacaca tatggcaata
     1921 aggctgattt gaaccccgtt ttgaatatga taatacaaaa ttatatttga gaaatttcaa
     1981 caaaatcgat aagagctata gctctctttt aattaaaaaa aactagataa aaagagcaat
     2041 ggtcagtggt ggtcagtggt cgttgtggcc tctatcgatt tccacacaaa aactttactg
     2101 tttagtaata gaaaagaact tgagaaaggc agccaaaaga atgtacgatc tgacgggtaa
     2161 gcatgtctgc tatgtggctg actgcggtgg cattgcactg gagaccagca aggttctcat
     2221 gaccaagaat atagcggtga gtggtagagt ggtgtagaga gtggagagtg ttccgaagtg
     2281 gttctggttc ttaaacaagt ctctagtctc tagtctcttg gctgctgctg gtagctgtcg
     2341 aaactaatta agttaatgac tttttcatgt tttgtttttg tccgctgact tttgctgttc
     2401 aaagctacaa ttaagtttat gttttgagtt tgatctactt ttgcttaagg gaagtacttt
     2461 cgattacgga ttgctggcat ataaaaatag ataccaagga atgtgcatca tatcatattg
     2521 attctatctt gattctttgc ccagaaactg gccatccttc agagcgtgga aaaccccccg
     2581 gccatcgccc agttacaatc gattaagcac agcacacaga tcttcttctg gaccttcgat
     2641 gtgaccatgg cccgcgagga gatgaagaag tacttcgatg aggtcatggt ccagatggac
     2701 tacatcgatg tgctgatcaa tggggccacc ctgtgcgatg agcggaacat tgatgccacc
     2761 atcaatacga atctgactgg catgatgaac accgtggcca ctgtgctgcc ctatatggac
     2821 cgtaagatgg gcggttcggg tggactgatt gtgaatgtca cctccgtcat aggactggat
     2881 ccatcgccag tcttttgtgc gtacagtgcc tcaaagtttg gtgtgattgg gttcacccga
     2941 agtctagcgg tgagtgggcg agatcttaac ttgaaaatgc tctctaatgt cttccattct
     3001 tccgcaggat cccctgtatt acacccaaaa tggtgtggct gtcatggccg tctgctgtgg
     3061 tcccaccaaa gtgtttgtcg atcgcgagct gactgccttc ctggcatacg gccaaacctt
     3121 tgccgatcgc ctgcgtcgtg ccccctgcca atcgacggcc gtctgcggcc agaatatagt
     3181 aaatgccatc gagagatcgg agaacggaca gatctggatt gccgacaagg gcggcctgga
     3241 gtcggtggcc ctccactggt attggcatat ggccgatcag tttgtgaact acatgcagag
     3301 cactgacgat gaggatcagg agtttttttt gggccagcga tgaggcttgc cttgatccac
     3361 ggaataaaac atcaaatgtg tcacaaaaag taggcaactt gtttgtgggt ctggaaatag
     3421 atttgaaata ttttgaggtc atatgaataa tgacataaat gagggtgcct gaaagtatgc
     3481 caagttt
//