From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Y.enterocolitica ampC and ampR genes for beta-lactamase and AmpR
Date: 10 Apr 92 10:14:50 GMT
Distribution: bionet
Checksum: 18456     7

LOCUS       YEPAMPCR     3143 bp ds-DNA             BCT       10-APR-1992
DEFINITION  Y.enterocolitica ampC and ampR genes for beta-lactamase and AmpR
            regulatory protein
ACCESSION   X63149
KEYWORDS    AmpR gene; AmpR regulatory protein; amp-C gene; beta-lactamase.
SOURCE      Yersinia enterocolitica DNA.
  ORGANISM  Yersinia enterocolitica
            Prokaryota; Bacteria; Gram-negative facultatively anaerobic rods;
            Enterobacteriaceae.
REFERENCE   1  (bases 1 to 3143)
  AUTHORS   Seoane,A., Francia,M.V. and Garcia Lobo,J.M.
  TITLE     Nucleotide sequence of the ampC-ampR region from the chromosome of
            Yersinia enterocolitica
  JOURNAL   Antimicrob. Agents Chemother. (1992) In press
  STANDARD  full automatic
COMMENT     *source: strain=IP97; *source: sub_strain=serotype 0:5b;
            From EMBL    entry YEAMPCR;  dated 30-MAR-1992.
FEATURES             Location/Qualifiers
     CDS             complement(529..1413)
                     /product="AmpR regulatory protein"
                     /gene="ampR"
                     /codon_start=1
                     /translation="MVRSYIPLNSLRAFEAAARQLSFTKAAIELNVTHAAISQQVKAL
                     EQRLNCRLFIRISRGLVLTTEGENLLPILNDSFDRIADTLDRFSTGIIREKVRVGVVG
                     TFATGYLLSRLRDFQQHSPHVDILLSTHNNRVDVVAEGLDYAIRYGNGALAWHESHFM
                     YAPPLAQLCAPSISKRFTPPTDLQRFMLLGSYRAMNWSAWFAAAGGSVPSPSQQIMMF
                     DSSVSMLEAAQAEIGIALAPPAMFMHLLRSERIIQPFSTTVSLGGYWLTRLQSRTETP
                     AMRDFALWLLSEMKSEGE"
     promoter        complement(1450..1478)
                     /gene="ampR"
     promoter        1461..1489
                     /gene="ampC"
     CDS             1548..2714
                     /EC_number="3.5.2.6"
                     /product="beta-lactamase"
                     /gene="ampC"
                     /translation="MMKKSIINTLIFTSIATFPLYTLAQTKLTELQVATIVNNTLTPL
                     LEKQGIPGMAVAVFYDGKPQFFNYGMADIKAGRPVTENTLFELGSVSKTFTGVAGEYA
                     MQTGIMNLNDPVTEYAPELTGSQWKDVKMLHLATYTAGGLPLQLPDSVTDQKSLWQYY
                     QQWQPQWAPGVMRNYSNASIGLFGALAVKRSQLTFENYMKEYVFQPLKLDHTFITIPE
                     SMQSNYAWGYKDGQPVRVTLGMLGEEAYGVKSTSQDMVRFMQANMDPESLPAGNDKLK
                     EAIIASQSRYFQAGDMFQGLGWEMYSWPINPQGVIADSGNDIALKPRKVEALVPAQPA
                     VRASWVHKTGATNGFGAYIVFIPEEKVGIVMLANKNYPNPVRVQAAYDILQALR"
BASE COUNT      883 a    697 c    758 g    805 t
ORIGIN
        1 gaattcgtca gaatcatcag gatgaaccac agtatggtca gcaagtcgcc ccgatagatc
       61 agcgtgtgca gcggttggtc atggcactgg tgtttgcggc caaaagtgat gggcatattg
      121 agcgaaagat gcgcagtatt attgagcaga atatgcgcga agcgggtatc agtgagcagg
      181 gtgaacgtct ggtgcaacag gcaattgacc agccattgga tcctcaatta ctggcgcgtg
      241 atattcaaaa tgaagaggaa gcgctggagc tatacttcct gagctgcgcc gtcattgatg
      301 tggaccattt tatggagcgt acgtatcttg ccgcgctagg gcacgcactg aaaattccgc
      361 aagatgttcg tgatggaatt gaacaagaca ttcgagagaa aaagcaaagc attaccgact
      421 aatttacttg caggcggcct ttggtattct ccaataggcc actgtttcag aagcgatgct
      481 tcgcagattt tggcggttca aacagtatta ttagacgtta atgcggatct attctccctc
      541 agacttcatt tcagacaata gccacagagc aaaatcccgc atagccggcg tctcagtgcg
      601 ggactgtagg cgagttaacc aatatccccc gagactgact gtcgtgctaa aaggttggat
      661 tatgcgttca ctccgtagca ggtgcataaa catagcgggt ggagccagcg cgatacctat
      721 ttctgcctgc gccgcttcca gcatactgac tgacgagtcg aacatcatta tttgctggga
      781 aggtgaaggt acagaccccc ccgcagcagc aaaccaagcc gaccaattca tcgcacggta
      841 ggaaccgagc agcataaacc tttgaagatc cgtaggtggc gtaaatcttt tgctaattga
      901 gggggcgcac agttgggcca aaggtggagc atacataaaa tgtgactcgt gccatgccag
      961 ggctccattg ccgtaacgaa tggcataatc cagtccttca gccacaacat cgacacgatt
     1021 attatgtgtt gagagtaaaa tatccacatg tggggaatgt tgttgaaaat ccctcaatcg
     1081 tgacaataaa tagccggtag caaaggtccc aacaacaccc accctgactt tttcacgtat
     1141 tattccagtg gagaaacggt ctagcgtatc cgcgatacga tcaaaagaat cgttcaggat
     1201 ggggagcaga ttctcccctt cggttgtgag cactaatccg cgtgaaatac gaataaagag
     1261 gcgacagttg aggcgttgtt ccagcgcttt gacctgctga ctgattgctg catgggtaac
     1321 atttaattcg atagcagcct tagtaaaact taactgtctg gccgcagcct cgaaagcgcg
     1381 aagtgaattt aacggaatat agctgcgaac catagattga cttgttagat tttctattat
     1441 caagtgctaa atataatcga ttgttatcca tagtcaatca ttgcagaatt cttcacgcaa
     1501 aaggagccac gctgcatacc atttatcagt ctatggaaga tttactaatg atgaaaaagt
     1561 ctataatcaa taccttgatc tttacttcta tcgcaacttt cccactttat accttggcac
     1621 aaaccaaact tacggagctg caggttgcga ctatcgtcaa taacaccctg acgccgttgc
     1681 tcgaaaaaca gggtattccc ggtatggccg tcgcagtatt ttatgacgga aagccgcaat
     1741 tctttaacta tggtatggcg gatataaaag caggtcgtcc ggtaaccgaa aatacactgt
     1801 ttgaactcgg ttcagtgagt aagaccttta ccggcgtcgc cggagaatat gccatgcaga
     1861 ccggtattat gaaccttaat gatcccgtga cggaatatgc tcccgaacta acgggcagcc
     1921 agtggaaaga tgtgaaaatg ctgcatctgg cgacgtacac tgcgggtgga cttcctctgc
     1981 aactccccga ttcggtgact gaccagaaat cattgtggca atattatcaa caatggcagc
     2041 cacagtgggc acctggggtg atgcgtaatt attctaatgc cagcattggg ctattcggtg
     2101 cgctggcggt gaaaagaagc cagttaacat ttgaaaacta tatgaaggaa tatgtgttcc
     2161 aacctttaaa actggatcat acctttatca ctatccccga atccatgcag tcaaattatg
     2221 cgtggggata taaagatggt caaccagtgc gcgttacctt gggtatgctg ggtgaggaag
     2281 cctatggagt gaaatccacc tcgcaagata tggtgagatt tatgcaggcc aatatggacc
     2341 cagaaagctt acctgccggt aacgataagc taaaagaagc gattatcgca tctcaatcac
     2401 gttatttcca ggcaggagat atgttccagg gcttgggatg ggaaatgtat agctggccta
     2461 tcaatccaca gggggtgatt gccgatagtg gcaatgatat tgcgttaaaa ccacgtaagg
     2521 ttgaggcatt ggtacctgca caacctgctg tgcgagcatc ctgggttcat aagaccggtg
     2581 caaccaatgg ctttggcgct tatattgtat tcatcccaga ggagaaggtt ggcatagtga
     2641 tgttagcgaa caagaattac cctaatccag tccgcgtaca agcagcatac gatattcttc
     2701 aagcattgcg ttgagatgac agacggctaa acatttctgc tgcgcttcta tgaaataagt
     2761 tataatattg tgaaaaaaaa tccacgcaat tgcgatttta tagcattttc gattcttatg
     2821 agattcagcg aaacctcatg agataacgaa ttttatttag acgttgaaca agaagttcat
     2881 cacatcgcca tcttttacga tgtaatcttt accttctgag cgcatcttcc cagcttcttt
     2941 tgcaccttgc tcacctttgt aggtaataaa gtcgtcaaaa gcgatagttt gggcgcggat
     3001 aaagcctttt tcaaagtcgg tgtgaatttt accggcagct tgtggcgctg ttgcacctac
     3061 agggatggtc caggcacgaa cttctttaac accggcggtg aagtaagttt gcagattcaa
     3121 caattcataa ccggcacgga tcc
//