From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: D.pseudoobscura Adh gene for alcohol dehydrogenase
Date: 28 Dec 92 21:26:43 GMT
Distribution: bionet
Checksum: 38745 8

LOCUS       DROADHGN22   3476 bp ds-DNA             INV       01-DEC-1992
DEFINITION  D.pseudoobscura Adh gene for alcohol dehydrogenase
ACCESSION   X62202
KEYWORDS    ADH gene; alcohol dehydrogenase.
SOURCE      Drosophila pseudoobscura DNA.
  ORGANISM  Drosophila pseudoobscura
            Eukaryota; Animalia; Metazoa; Arthropoda; Uniramia; Insecta;
            Pterygota; Neoptera; Holometabola; Diptera; Brachycera;
            Cyclorrhapha; Schizophora; Drosophiloidea; Drosophilidae.
REFERENCE   1  (bases 1 to 3476)
  AUTHORS   Schaeffer,S.W. and Miller,E.L.
  TITLE     Estimates of gene flow in Drosophila pseudoobscura determined from
            nucleotide sequence analysis of the alcohol dehydrogenase region
  JOURNAL   Genetics 132, 471-480 (1992)
  STANDARD  full automatic
COMMENT     *source: strain=GB26; *source: isolate=Gundlach Bundschu Winery,
            Sonoma Valley, *source: California; *source: chromosome=IV section
            88;
               From EMBL    entry DPADHGN22;  dated 16-OCT-1992.
FEATURES             Location/Qualifiers
     mRNA            join(40..119,916..1021,1085..1489,1552..1985)
                     /note="adult mRNA (Adh)"
     mRNA            join(883..1021,1085..1489,1552..1985)
                     /note="larval mRNA (Adh)"
     promoter        8..14
                     /note="adult promoter (Adh)"
     prim_transcript 40..1985
                     /note="adult primary transcript (Adh)"
     exon            40..119
                     /note="adult exon 1a"
     intron          120..915
                     /note="adult intron"
     promoter        847..854
                     /note="larval promoter (Adh)"
     prim_transcript 883..1985
                     /note="larval primary transcript (Adh)"
     exon            883..1021
                     /note="larval exon 1"
     exon            916..1021
                     /note="adult exon 1b"
     intron          1022..1084
                     /number=1
     exon            1085..1489
                     /number=2
     intron          1490..1551
                     /number=2
     exon            1552..1985
                     /number=3
     polyA_signal    1962..1967
     polyA_site      1985
     CDS             join(929..1021,1085..1489,1552..1818)
                     /EC_number="1.1.1.1"
                     /gene="Adh"
                     /product="alcoholdehydrogenase"
                     /codon_start=1
                     /translation="MSLTNKNVVFVAGLGGIGLDTSRELVKRNLKNLVILDRIDNPAA
                     IAELKAINPKVTITFYPYDVTVPVAETTKLLKTIFAQVKTIDVLINGAGILDDHQIER
                     TIAVNYTGLVNTTTAILDFWDKRKGGPGGIICNIGSVTGFNAIYQVPVYSGSKAAVVN
                     FTSSLAKLAPITGVTAYTVNPGITKTTLVHKFNSWLDVEPRVAEKLLEHPTQTSQQCA
                     ENFVKAIELNKNGAIWKLDLGTLEPITWTQHWDSGI"
BASE COUNT      981 a    791 c    791 g    913 t
ORIGIN      
        1 cgtagtgtat ttaaggcact gcacatgtcg aggaccacga ttattgtcta cagtgcagtc
       61 agtagttgtc aggtgcagca ggcggaccag catttcttta aatctactca atcgatcaag
      121 taagtaactg taatccaagt acagtcggcc catcccactc ggttctgcag taattcaaga
      181 aatttcttga tagcgcagtt ccagttgtaa aacgtcgttt aaaaaaacat ctcgttggag
      241 cttgggttga aaaacgttcg agttgggcgt agaaaagctc aaatataaaa tatcaaaaaa
      301 gagaaaatat cttcccaaaa gcagctgtcc attttttgtt aaagtgatag atcaaaaatt
      361 cgtatgacgt atttcccaag tgctcttcat gcaaataatg tcctcaattc taattctaca
      421 agaagcactt ttggctcttg tccagaaaaa acaatttgtt tacacttttc ggttgataac
      481 agaagcggac cgacggctaa gtaacataac agtcccctgt taagtaatgc cctcactctc
      541 tgttagatgg ccgcgcgtgt cgactccgat agaccagcag acacgcagta gttttcctca
      601 agaacctgaa ctctaaacat agacataatt tacgatacct tgagactcaa ataaaaatac
      661 aagtatgagt ggtattctat tcctgattag actcgcacac acacacgtac gagtattaac
      721 ataaatttgc cggcaaataa ccaaaacaaa acgaacgcaa acgatccgag cacgtaatgc
      781 gagagataag aaacagaaaa gctcgacgtg aaagaagctt ctggcggaag ctcttgccga
      841 actccctata aatacgagac agaaaaccag aataaatata ataacaaact gttgaattgt
      901 cccatcgatt cccagatcaa aagacagaat gtctctcacc aacaagaacg tcgttttcgt
      961 ggcgggtctg ggcggcattg gcctggacac cagccgggag ttggtcaagc gtaatctgaa
     1021 ggtaagagag aaagaattcc atggaatcta tggaatccta aatttaaaaa tttatttttt
     1081 ttagaacctg gtcatcctgg atcgcattga caatccggct gccattgccg aactgaaggc
     1141 aatcaatccc aaggtgacca tcaccttcta tccctacgat gtgactgtgc ccgtcgctga
     1201 gaccaccaag ctcctgaaga ccatctttgc ccaggtgaag acaatcgatg tcctgatcaa
     1261 cggtgctggc atcctggacg atcatcagat cgagcgcacc attgccgtta actacacggg
     1321 cctggtcaac accaccacag ccattctgga cttctgggac aagcgcaagg gcggcccagg
     1381 cggcatcatt tgcaacattg gctccgtcac cggtttcaat gccatctacc aggtgcccgt
     1441 ttactccggc tccaaggcgg cggtggttaa ctttacctcc tccctggcgg taagaaatcc
     1501 tacaactatc ctaactatcc caaaactaaa tatttcttta aaatcccata gaaactggct
     1561 cccattactg gtgtcactgc ttacactgtc aatcctggca tcactaagac cactctggtc
     1621 cacaaattca actcgtggct ggatgtggag ccccgtgtgg cagagaagct gctcgagcat
     1681 cccacccaga cctctcagca gtgcgccgag aactttgtga aggccattga gctgaacaag
     1741 aacggtgcca tctggaagtt ggatctgggc accttggagc ccatcacatg gacccagcac
     1801 tgggattcgg gcatctaaac catcccagag actctatggg acatggcgtt ttagctttta
     1861 gtttcttttt ttccactcaa ttgttacgta tatacataca catatggcaa taaggctgat
     1921 ttgattctct tttatggaag gaaccccgtt ttgaatatga taatacaaaa ttatatttga
     1981 gaaatttcaa caaaatcgat aagagctcta gctctctttt aattaaaaaa aactagataa
     2041 aaagagcaat ggtcagtggt ggtcagtggt cgttgtggcc tctatcgatt tccacacaaa
     2101 aactttactg tttagtaata gaaaagaact tgagaaaggc agccaaaaga atgtacgatc
     2161 tgacgggtaa gcatgtctgc tatgtggctg actgcggtgg cattgcactg gagaccagca
     2221 aggttctcat gaccaagaat atagcggtga gtggtagaga ggtggtgtag agagtggttc
     2281 tggttcttaa acaagtctct agtctcttgg ctgctgctgg tagctgtcga aactaattaa
     2341 gttaatgact ttttcatgtt ttgtttttgt ccgctgactt ttgctgttca aagctacaat
     2401 taagtttatg ttttgagttt gatctacttt tgcttaaggg aagtactttc gattacggat
     2461 tgctggcata taaaaataga taccaaggaa tgtgcatcat atcatattga ttctatcttg
     2521 attctttgcc cagaaactgg ccatccttca gagcgtggaa aaccccccgg ccatcgctca
     2581 gttacaatcg attaagcaca gcacacagat cttcttctgg accttcgatg tgaccatggc
     2641 ccgcgaggag atgaagaagt acttcgatga ggtcatggtc cagatggact acatcgatgt
     2701 actgatcaat ggggccaccc tgtgcgatga gcggaacatt gatgccacca tcaatacgaa
     2761 tctgaccggc atgatgaaca ccgtggccac tgtgctgccc tatatggacc gaaagatggg
     2821 cggttcgggt ggactgatcg tgaatgtcac ctccgtcata ggactggatc catcgccagt
     2881 cttttgtgcg tacagtgcct caaagtttgg tgtgattggg ttcacccgaa gtctagcggt
     2941 gagtgggcga gatcttaact tgaaaatgct ctctaatgtc ttccattctt tcgcaggatc
     3001 ccctgtatta cacccaaaat ggtgtggctg tcatggccgt ctgctgtggt cccaccaaag
     3061 tgtttgtcga tcgggaactg actgccttcc tggcatacgg ccaaaccttt gccgatcgcc
     3121 tgcgtcgtgc cccctgccaa tcgacggccg tctgcggcca gaatatagta aatgccatcg
     3181 agagatcgga gaacggacag atctggattg ccgacaaggg cggcctggag tcggtggccc
     3241 tccactggta ttggcatatg gccgatcagt ttgtgaacta tatgcagagc actgacgatg
     3301 aggatcagga gttttttttg ggccagcgat gaggcatgcc ttgatccacg gaataaaaca
     3361 tcaaatgtgt cacaaaaagt aggcaacttg tttgtgggtc tggaaataga tttgaaatat
     3421 tttgaggtca tatgaataat gacataaatg agggtgcctg aaagtatgcc aagttt
//