From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Bacillus sp. methanol dehydrogenase (mdh) gene, complete cds.
Date: 4 Sep 92 10:02:32 GMT
Distribution: bionet
Checksum: 34014     6

LOCUS       BACMDH       2466 bp ds-DNA             BCT       04-SEP-1992
DEFINITION  Bacillus sp. methanol dehydrogenase (mdh) gene, complete cds.
ACCESSION   M65004
KEYWORDS    activator protein; methanol dehydrogenase.
SOURCE      Bacillus sp. (strain C1) DNA.
  ORGANISM  Bacillus sp.
            Prokaryotae; Firmicutes; Endospore-forming rods and cocci;
            Bacillaceae.
REFERENCE   1  (bases 1 to 2466)
  AUTHORS   de Vries,G.E., Arfman,N., Terpstra,P. and Dijkhuizen,L.
  TITLE     Cloning, expression, and sequence analysis of the Bacillus
            methanolicus C1 methanol dehydrogenase gene
  JOURNAL   J. Bacteriol. 174, 5346-5353 (1992)
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     promoter        661..690
                     /gene="mdh"
                     /note="putative"
                     /citation=[1]
     CDS             729..1874
                     /function="methylotrophy"
                     /EC_number="1.1.1.1"
                     /product="methanol dehydrogenase alpha-10 subunit"
                     /gene="mdh"
                     /note="putative"
                     /codon_start=1
                     /translation="MTNFFIPPASVIGRGAVKEVGTRLKQIGAKKALIVTDAFLHSTG
                     LSEEVAKNIREAGLDVAIFPKAQPDPADTQVHEGVDVFKQENCDALVSIGGGSSHDTA
                     KAIGLVAANGGRINDYQGVNSVEKPVVPVVAITTTAGTGSETTSLAVITDSARKVKMP
                     VIDEKITPTVAIVDPELMVKKPAGLTIATGMDALSHAIEAYVAKGATPVTDAFAIQAM
                     KLINEYLPKAVANGEDIEAREAMAYAQYMAGVAFNNGGLGLVHSISHQVGGVYKLQHG
                     ICNSVNMPHVCAFNLIAKTERFAHIAELLGENVSGLSTAAAAERAIVALERYNKNFGI
                     PSGYAEMGVKEEDIELLAKNAFEDVCTQSNPRVATVQDIAQIIKNAL"
                     /citation=[1]
     protein_bind    762..839
                     /bound_moiety="nicotinamide-adenine dinucleotide (NAD)"
                     /gene="mdh"
                     /note="putative"
                     /citation=[1]
     terminator      1885..1921
                     /gene="mdh"
                     /citation=[1]
BASE COUNT      864 a    444 c    468 g    690 t
ORIGIN
        1 ggatccgtcc ataccattct gagtcggatg atgaataaga atgctgtcat cgttttcttt
       61 tatggaaatg tcgggtacat atacattaaa aaagaccgcc gatgatacaa caccaaacgg
      121 ttatgtaata aaaggttccc ttaaaagagg gatatcagct aaccaaagaa aagtaaatcc
      181 agacctttaa cgcttaccgg ctcaaggatg gatttacttt ttttatgtta aagcaatata
      241 tcgatctagc ttaaaaacga aatagcgaac atcaatgctt gaaaaacggt cagaagccgc
      301 tctctctttc gggaatactg ctaccgttga gtttatctat atttattgcc tattctatta
      361 ctcttttatt ataaaagctt caagttgacc acaaaaaaac tttcattttt taagacaatt
      421 tgatgcctta ggatctagtc gatcagtcgt tttatgatat aactttaatc ctttttacaa
      481 agtcaaattt aaatcctgcc cttccacttt aatcctccac actatataag cgtcaaaatc
      541 actttttctt tggaaatctt ttattaggtt gtttttccct tgagcttttt atataaaatt
      601 aatattcaga tccttctaaa aaatataatt tcgaaaacta agaacattac gacaactata
      661 ttgacaaaca tttagattag catttaaact agttttgtaa acaattacat aaataggagg
      721 tagtaagaat gacaaacttt ttcattccac cagccagcgt aattggacga ggtgcagtaa
      781 aggaagtagg aacaagactt aagcaaattg gagctaagaa agcgcttatc gttacagatg
      841 catttcttca tagcacaggt ttatctgaag aagttgctaa aaacattcgt gaagctggcc
      901 ttgatgttgc gattttccca aaagctcaac cagatccagc agatacacaa gttcatgaag
      961 gtgtagatgt attcaaacaa gaaaactgtg atgcacttgt ttctatcggt ggaggtagct
     1021 ctcacgatac agctaaagca atcggtttag ttgcagcaaa cggcggaaga atcaatgact
     1081 atcaaggtgt aaacagtgta gaaaaaccag tcgttccagt agttgcaatc actacaacag
     1141 ctggtactgg tagtgaaaca acatctcttg cagttattac agactctgca cgtaaagtaa
     1201 aaatgcctgt tattgatgag aaaattactc caactgtagc aattgttgac ccagaattaa
     1261 tggtgaaaaa accagctgga ttaacaatcg caactggtat ggacgcatta tcacacgcaa
     1321 ttgaagcata tgttgcaaaa ggtgctacac cagttactga tgcatttgca attcaagcaa
     1381 tgaaactcat caatgaatac ttaccaaaag cggtggcaaa cggagaagac atcgaagcac
     1441 gtgaagcaat ggcttatgca caatacatgg caggagtggc atttaacaac ggtggtttag
     1501 gattagtaca ctctatttct caccaagtag gtggagttta caaattacaa cacggaatct
     1561 gtaactcagt taatatgcca cacgtttgcg cattcaacct aattgctaaa actgagcgct
     1621 tcgcacacat tgctgagctt ttaggcgaga atgtttctgg cttaagcact gcagcagctg
     1681 ctgagagagc aattgtagcg cttgaacgct ataacaaaaa cttcggtatc ccatctggct
     1741 atgcagaaat gggcgtgaaa gaagaggata tcgaattatt agcgaaaaac gcattcgaag
     1801 acgtatgtac tcaaagcaac ccacgtgttg ctacagttca agacattgca caaatcatca
     1861 aaaacgctct gtaatcataa tcttggaaaa gcgtctcttg aaattcaaga gacgcttttc
     1921 cgtgatgcta gattaaatag tagacacaga ttaatagagt aatagactaa agaatgagga
     1981 gactacatgt cgaaaaacaa aggcggtacg aggaagattt taaaaaatat atcgaatccc
     2041 gccaactgta taggagttca aaagttatga aaaagctcaa tcatgaagga attgatgttt
     2101 ctcagaagac ggtccaatga aagatgaagg aatgaagtct cgtaccgtaa aaaaataaaa
     2161 ccacacggtc catgaaaatg tcttaaacaa taaagtgtgg gtgaatgagg aaaaactaca
     2221 tggaaatcgg tcttgaaaac gaaataaaaa ttagaaaaac tctctctcgt ttaatacagt
     2281 atactattcg atactatatg ttaataaagt gcatacttac gaattgatat tatacacgtt
     2341 atactagatt ttgtataggt aatatcgaat atagaaagtc ataaataaca caggaggtga
     2401 tcctctagag tcgacctgca ggcatgcaag cttttgttcc ctttagtgag ggttaatttc
     2461 gagctt
//