From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: N.tabacum mRNA for pre-pro-cysteine proteinase
Date: 28 Jul 92 10:08:02 GMT
Distribution: bionet
Checksum: 22041     5

LOCUS       TOBCYP7      1304 bp ss-RNA             PLN       28-JUL-1992
DEFINITION  N.tabacum mRNA for pre-pro-cysteine proteinase
ACCESSION   Z13959
KEYWORDS    cysteine proteinase.
SOURCE      Nicotiana tabacum RNA.
  ORGANISM  Nicotiana tabacum
            Eukaryota; Plantae; Embryobionta; Magnoliophyta; Magnoliopsida;
            Asteridae; Solanales; Solanaceae.
REFERENCE   1  (bases 1 to 1304)
  AUTHORS   Linthorst,H.J.
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
REFERENCE   2  (bases 1 to 1304)
  AUTHORS   Linthorst,H.J., Van der Does,C., Brederode,F.T. and Bol,J.F.
  TITLE     Circadian expression and induction by wounding of tobacco genes for
            cysteine proteinase
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
COMMENT     *source: strain=cv. Samsun NN; *source: stage=Acutely infected;
            *source: tissue=Leaf; *source: clone_library=Lambda ZAP
            TMV-tobacco; *source: clone=CYP-7;
            From EMBL    entry NTCYP7;  dated 29-JUN-1992.
FEATURES             Location/Qualifiers
     CDS             7..1098
                     /function="Putative precursor of cysteineproteinase"
                     /product="tobacco pre-pro-cysteineproteinase"
                     /codon_start=1
                     /translation="MERLFLLSLLAFVLFSSAIAFSDEDPLIRQVVSETDDSHLLNAE
                     HHFSLFKSKFGKIYASEEEHDHRFKVFKANLRRARLNQLLDPSAEHGITKFSDLTPSE
                     FRRTYLGLHKPKPKLNAEKAPILPTSDLPADFDWRDHGAVTGVKNQGSCGSCWSFSTT
                     GAVEGAHFLATGELVSLSEQQLVDCDHECDPEQQDACDAGCGGGHYATAFEYTLKAGG
                     LQLEKDYPYTGKDGKCHFDKSKICAAVTNFSVIGLDEDQIAANLVKHGPLAVGINAAW
                     MQTYVGGVSCPLICFKRQDHGVLLVGYGSHGFAPIRLKEKAYWIIKNSWGENWGEHGY
                     YKICRGHNICGVDAMVSTVTAAHTTNPNL"
     sig_peptide     7..60
     mat_peptide     394..1095
                     /function="protein degradation"
                     /note="Matureprotein putatively processed from
                     pre-pro-protein,based on similarity to other cysteine
                     proteinases"
                     /product="tobacco cysteine proteinase"
     misc_feature    466..468
                     /product="encodes active Cys residue"
     misc_feature    891..893
                     /product="encodes active His residue"
     polyA_site      1304
                     /evidence=EXPERIMENTAL
                     /citation=[1]
                     /citation=[2]
BASE COUNT      320 a    295 c    318 g    371 t
ORIGIN
        1 ctccaaatgg aacgtctatt tctcttatct cttctcgctt tcgtactttt ctcgtcggcg
       61 atagcgttct ccgacgagga tccgttgatc cggcaagtcg tgtccgaaac cgacgacagt
      121 catctgttaa acgccgaaca tcacttttca cttttcaagt caaagttcgg aaagatctac
      181 gcttcggaag aggaacatga ccacaggttc aaggtattca aggcgaacct ccgccgtgca
      241 cggctcaatc agctccttga cccttccgcc gaacacggta ttacgaagtt ctccgatctt
      301 actccgtcgg agtttcgccg gacttacctt ggccttcaca aaccaaaacc taagcttaac
      361 gctgagaaag ctccgatcct cccgacttcc gatcttccag ctgattttga ctggcgtgac
      421 cacggcgccg tcaccggcgt aaaaaatcag ggctcgtgcg gttcatgctg gtcgttcagt
      481 acaacaggag cggtggaagg agctcacttt ttggcgacgg gagagctggt gagccttagt
      541 gaacagcagc ttgtggactg tgaccatgag tgtgacccgg agcaacaaga tgcttgtgat
      601 gcaggttgcg gtggtggtca ttatgctact gcctttgagt acaccctgaa agctggaggt
      661 cttcaactgg agaaagacta tccttacact ggcaaggatg gcaagtgcca ctttgacaag
      721 agcaaaatct gtgccgctgt gactaacttc agtgttattg gtcttgatga agaccaaatt
      781 gctgccaacc tggttaaaca tggtcctctt gcagtgggga tcaatgctgc ttggatgcag
      841 acatacgttg gaggagtttc gtgcccatta atctgcttca agcgtcagga tcacggagtg
      901 ctcttggtag gttatggttc tcatggcttt gctcctatca gactcaagga aaaggcttac
      961 tggatcatca agaattcatg gggagagaac tggggagagc atggatatta caagatttgc
     1021 aggggtcaca atatctgtgg agttgatgcc atggtctcta ctgtgactgc tgcccatact
     1081 actaatccta acctgtgaga tgaacttatc cgaggaatgt tgtctgtgat caagggtttt
     1141 actacattat cctaatttgt gtaaatatgt tatttagtaa tgtattgtga actcaagtct
     1201 tagatttcat cagcacttta ttatcgtgct tcaaaccacg tagtggattt gtatttctgt
     1261 agtttatact gcaaaaatga agttcgaatt ttaaagttgt ttgt
//