From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: D.melanogaster Tpi gene for Triosephosphate isomerase
Date: 28 Dec 92 21:28:13 GMT
Distribution: bionet
Checksum: 12578 7

LOCUS       DROTPIG      3419 bp ds-DNA             INV       01-DEC-1992
DEFINITION  D.melanogaster Tpi gene for Triosephosphate isomerase
ACCESSION   X57576
KEYWORDS    glycolytic enzyme; tpi gene; triosephosphate isomerase.
SOURCE      Drosophila melanogaster DNA.
  ORGANISM  Drosophila melanogaster
            Eukaryota; Animalia; Metazoa; Arthropoda; Uniramia; Insecta;
            Pterygota; Neoptera; Holometabola; Diptera; Brachycera;
            Cyclorrhapha; Schizophora; Drosophiloidea; Drosophilidae.
REFERENCE   1  (bases 1 to 3419)
  AUTHORS   Shaw-Lee,R.L., Lissemore,J.L. and Sullivan,D.T.
  TITLE     Structure and expression of the triose phosphate isomerase (Tpi)
            gene of Drosophila melanogaster
  JOURNAL   Mol. Gen. Genet. 230, 225-229 (1991)
  STANDARD  full automatic
COMMENT     FLYBASE; 03738; Tpi.
               *source: strain=Oregon-R; *source: clone_library=EMBL-4;
            *source: is_germline=Y;
               From EMBL    entry DMTPIG;  dated 20-JAN-1992.
FEATURES             Location/Qualifiers
     mRNA            join(2004..2028,2186..2773,2830..3036)
                     /gene="Tpi"
     prim_transcript 2004..3296
     exon            2004..2028
                     /number=1
     intron          2029..2185
                     /number=1
     misc_feature    2147..2151
                     /note="intron 1 lariat sequence"
     exon            2186..2773
                     /number=2
     intron          2774..2824
                     /number=2
     misc_feature    2789..2793
                     /note="intron 2 lariat sequence"
     exon            2825..3296
                     /number=3
     polyA_signal    3253..3257
     CDS             join(2237..2773,2830..3036)
                     /EC_number="5.3.1.1"
                     /gene="Tpi"
                     /product="triosephosphate isomerase"
                     /codon_start=1
                     /translation="MSRKFCVGGNWKMNGDQKSIAEIAKTLSSAALDPNTEVVIGCPA
                     IYLMYARNLLPCELGLAGQNAYKVAKGAFTGEISPAMLKDIGADWVILGHSERRAIFG
                     ESDALIAEKAEHALAEGLKVIACIGETLEEREAGKTNEVVARQMCAYAQKIKDWKNVV
                     VAYEPVWAIGTGKTATPDQAQEVHASLRQWLSDNISKEVSASLRIQYGGSVTAANAKE
                     LAKKPDIDGFLVGGASLKPEFLDIINARQ"
BASE COUNT      855 a    933 c    849 g    778 t      4 others
ORIGIN      
        1 gatctcgagc gagaaatgtg gaacatagtg gaggcctcca gtggcgccga gctgggtgaa
       61 accagctacg agttcccttc ccccgctccg gttcccagcg cagcagtgaa cgaaatagca
      121 gttccacagt cccaccagct cctcctgctc ctgcgaagcc ctcagttccg tccgcctcct
      181 atgacaacca caactacagt ttcagccagg atgaggacga agatgatgat gatctggagt
      241 ttgaggacgt attcgtgccg gccagctctg ttccaaatcc cgttcagcct ggcatagatc
      301 ccgtggaact gcgtcgctcc ctggctttgg tcatgaggga gaaattgcga tcggatgaca
      361 cggactccag gccaatgggc aacaatcagg atcttcccat agatgaacag tccagggaga
      421 gaccgctctc cactcaaaca tctcccacaa atggcccact tccggctctt ctgagggcca
      481 aactgcttgc tgggcaactc nnnncaatag cgctcactgc ctgccaggat ccacggcgag
      541 tcctgctccc caggagcaat ccggtatctt tgtgatcgat agtgaggcga gtcccggctc
      601 aaatgggcac aagcctaagt atcgaaaggg cacggcattc actcggagtt cgctgaagaa
      661 gagccgatcc tgcaactgta gctccatcgc taagggacga ggggtccacg acgagcccag
      721 cagtaatctc tgcagggatc aggagtcctc tgtacttcca cagcatccgc agccagccaa
      781 ccatcccaca gagaactttt ttagtccgct gagatcggtg ggcagtttca tgcagcattc
      841 caacttgttt cacttcctcc agcctcacgc cgcccgtccc acctcatcga cggcgtcgtc
      901 cacggcctcc acgcccactc catcaccacc gcccatgggc caggctcagg aggaatcggt
      961 tccagtgggt ctgaccacct cctcgccgtc ccttctgcga ccagcgcgga gagttgacct
     1021 tcgcggtcgg aaaagaagcg ggatcagcag gaaagtccac tggaagagga tcacaaggat
     1081 gcgaaagggg taagggtgac ccctgaggaa atactcgcaa gtcaaagctt ttacgttaag
     1141 tactttccag ctctcttagg ctctctttcg gaaacgttgt caaattctat atcggatcta
     1201 gatcaattag ttgggtagtg acatatagag attaggagct atgcgctttt attttgagtg
     1261 tatggtcata atacgtttct acggactagt taattatgta catacatatg tttaatgttg
     1321 ttagagtatt tactgtgtat atgtcagctt atgtatacct gaacgtctgt gaaaaccaag
     1381 ttaagccaac atgaatttca aagttgaagt taaataaagc gattaaacca gaatgtacat
     1441 tcatgttgtg ttttattttt gttggttaaa taagtcctga tgcgatgtat gcctaatggt
     1501 agggtataca agtttttctt ttacatatgc tagtatagcg gattttggca agcctgttca
     1561 taaaagcttt tcgagagctt tttggaagcg acgccaaaac tgttggagcc caagttcccc
     1621 attagatggt tggagtcgct gctaaatcta aaaccggcaa catttttaaa accccaacaa
     1681 gccgtttata taatttcaga ataacttttt tactacttct gacgaacaca aattggttac
     1741 cacaatagaa atgccgcctg gagtactcaa tgatctgaac aggaacggta tccagtgcat
     1801 tgcatcagta acgcttctcc agcgattccc gtatcatcgg aaccaccaca ccatccccac
     1861 catggaccag aaccgaccca ctgtgggtgg tgttacggcg gaggtactaa aagccgtcgt
     1921 ttcggaagct ctgctgggca aattcacagc tgttttccaa agcttttcaa gtgccagtgg
     1981 aagacttacg aaggtcaaaa gaaggttagt acataagcag acgaacttcg aggtgggtgt
     2041 gtgctctgtg ccgggattaa tctcgaatct gggaaaaatc tgagtggaaa agtcgacggc
     2101 gagcctccag tcatcgagtt acccacttga aattatcagt tccaaacact ctaatagcag
     2161 tccccttgtt ttgtcccccg atccgcagtt ctacgccaat ttcagcaccg attgcaccga
     2221 cagcaacagc aacaacatga gccgaaagtt ctgcgtggga ggcaactgga agatgaacgg
     2281 cgaccagaag tccatcgccg agatcgccaa gaccctgagc tcggccgccc tcgaccccaa
     2341 cacggaggtg gtcatcggct gcccggccat ctacctgatg tacgcccgca acctgctgcc
     2401 ctgcgagctg ggtctggccg gccagaatgc ctacaaggtg gccaagggcg cattcaccgg
     2461 cgagatctcc cctgcgatgc tgaaggacat cggcgccgac tgggtgatcc tgggacactc
     2521 ggagcgtcgc gccatcttcg gcgaatcgga cgccctgatc gccgagaagg ccgagcacgc
     2581 cctggccgag ggcctcaagg tcatcgcctg cattggtgag accctggagg agcgcgaggc
     2641 cggcaagacc aacgaggtgg tggcccgcca aatgtgcgcc tacgcccaaa agatcaagga
     2701 ctggaagaac gtggtggtgg cctacgaacc cgtctgggcc attggcaccg gcaagaccgc
     2761 cacacccgat caggtaaggt cgattgcaga tggctaatcc ttcagaacta tatttccttc
     2821 caattgcagg ctcaagaggt ccacgcttcc ctgcgccagt ggctgagcga caacatctcc
     2881 aaggaggtgt ccgccagcct gcgcatccag tacggtggat ccgtgaccgc cgccaacgcc
     2941 aaggagctgg ccaagaagcc cgacatcgat ggcttcctgg tcggtggcgc ctccctgaag
     3001 cccgagttct tggacatcat caacgcccgg cagtaagcaa cgcgatctca agcatcagat
     3061 cgctttcggc cagcgaactg cccgcgaaga ggcactggag ggcaggactc catactcctg
     3121 cgcgcgaatg cattacgcac acacctagtt attgtcacca ccaagtgcag tccaaatcat
     3181 agttaccttg cgagaatgct actcacatcc tgtgtgaacg tatagtagtc catgtgtaaa
     3241 tcttaaatta ttcaggcaat aattatgcgc tactcattta cttatatacc aaaccaaaca
     3301 ggtgtcttat tttttgcaag actgtttcag ctattttaga gcgattggtt gaacgacaag
     3361 tgtcgtgtca cctctgcgga ccttaaaccg aacaagttat tacttaattt ctgataagc
//