From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: D.pseudoobscura Adh gene for alcohol dehydrogenase
Date: 28 Dec 92 21:27:58 GMT
Distribution: bionet
Checksum: 03823 8

LOCUS       DROADHGN72   3494 bp ds-DNA             INV       01-DEC-1992
DEFINITION  D.pseudoobscura Adh gene for alcohol dehydrogenase
ACCESSION   X64481
KEYWORDS    ADH gene; alcohol dehydrogenase.
SOURCE      Drosophila pseudoobscura DNA.
  ORGANISM  Drosophila pseudoobscura
            Eukaryota; Animalia; Metazoa; Arthropoda; Uniramia; Insecta;
            Pterygota; Neoptera; Holometabola; Diptera; Brachycera;
            Cyclorrhapha; Schizophora; Drosophiloidea; Drosophilidae.
REFERENCE   1  (bases 1 to 3494)
  AUTHORS   Schaeffer,S.W. and Miller,E.L.
  TITLE     Estimates of gene flow in Drosophila pseudoobscura determined from
            nucleotide sequence analysis of the alcohol dehydrogenase region
  JOURNAL   Genetics 132, 471-480 (1992)
  STANDARD  full automatic
COMMENT     *source: strain=PS265; *source: isolate=Kiabab National Forest,
            Arizona; *source: chromosome=IV section 88;
               From EMBL    entry DPADHGN72;  dated 16-OCT-1992.
FEATURES             Location/Qualifiers
     mRNA            join(40..119,915..1020,1084..1488,1549..1982)
                     /note="adult mRNA (Adh)"
     mRNA            join(882..1020,1084..1488,1549..1982)
                     /note="larval mRNA (Adh)"
     promoter        8..14
                     /note="adult promoter (Adh)"
     prim_transcript 40..1982
                     /note="adult primary transcript (Adh)"
     exon            40..119
                     /note="adult exon 1a"
     intron          120..914
                     /note="adult intron"
     prim_transcript 882..1982
                     /note="larval primary transcript (Adh)"
     exon            882..1020
                     /note="larval exon 1"
     exon            915..1020
                     /note="adult exon 1b"
     intron          1021..1083
                     /number=1
     exon            1084..1488
                     /number=2
     intron          1489..1548
                     /number=2
     exon            1549..1982
                     /number=3
     polyA_signal    1959..1964
     polyA_site      1982
     CDS             join(928..1020,1084..1488,1549..1815)
                     /EC_number="1.1.1.1"
                     /gene="Adh"
                     /product="alcoholdehydrogenase"
                     /codon_start=1
                     /translation="MSLTNKNVVFVAGLGGIGLDTSRELVKRNLKNLVILDRIDNPAA
                     IAELKAINPKVTITFYPYDVTVPVAETTKLLKTIFAQVKTIDVLINGAGILDDHQIER
                     TIAVNYTGLVNTTTAILDFWDKRKGGPGGIICNIGSVTGFNAIYQVPVYSGSKAAVVN
                     FTSSLAKLAPITGVTAYTVNPGITKTTLVHKFNSWLDVEPRVAEKLLEHPTQTSQQCA
                     ENFVKAIELNKNGAIWKLDLGTLEPITWTQHWDSGI"
BASE COUNT      974 a    796 c    800 g    924 t
ORIGIN      
        1 cgtagtgtat ttaaggcact gcacatgtcg aggaccacga ttattgtcta cagtgcagtc
       61 agtagttgtc aggtgcagca ggcggaccag aatttcttta aatctactca atcgatcaag
      121 taagtaactg taatccaagt acagtcggcc catcccactc ggttctgcag taactcaaga
      181 aatttcttga tagcgcagtt ccagttgtaa aacgtcgttt aaaaaaacat ctcgttggag
      241 cttgggttga aaaacgttcg agttgggcgt agaaaagttc aaatataaaa tatcaaataa
      301 gagaaaatat cttcccaaag cagctgtcca ttttttgtta aagtgataga tcaaaaattc
      361 gtatgacgta tttcccaagt gctctttatg caaataattt cctcaattct aattctacaa
      421 gaagcacttt tggctcttgt ccagaaaaaa caatttgttt acactttgcc gttgataaca
      481 gaagcggacc gacggctaag taacataaca gtcgcctgtt aagtaatgcc ctcactctct
      541 gttagatggc cgcgcgtgtc gactccgata gaccagcaga cacgcagtag ttttcctcaa
      601 gaacctgaac tctaaacata gacataattt acgatacctt gagactcaaa taaaaataca
      661 agtatgagtg gtattctatt cctgattaga ctcgcacaca cacacgtacg agtattaaca
      721 taaatttgcc ggcaaataac caaaacaaaa cgaacgcaaa cgatccgagc acgtaatgcg
      781 agagataaga aacagaaaag ctcgacgtga aagaagcttc tggcggaagc tcttgccgaa
      841 ctccctataa atacgagaca gaaaaccaga ataaatataa taacaaactg ttgaattgtc
      901 ccatcgattc ccagatcaaa agacagaatg tctctcacca acaagaacgt cgttttcgtg
      961 gccggtctgg gcggcattgg cctggacacc agccgggagt tggtcaagcg taatctgaag
     1021 gtaagagaga acgaattcca tggaatctat ggaatcctaa atttaaaaat tcatttattt
     1081 tagaacctgg tcatcctgga tcgcattgac aatccggctg ccattgccga actgaaggca
     1141 atcaatccca aggtgaccat caccttctat ccctacgatg tgactgtgcc cgtcgctgag
     1201 accaccaagc tcctgaagac catctttgcc caggtgaaga caatcgatgt cctgatcaac
     1261 ggtgctggca tcctggacga tcatcagatc gagcgcacca ttgccgttaa ctacacgggc
     1321 ctggtcaaca ccaccacagc cattctggac ttctgggaca agcgcaaggg cggcccaggc
     1381 ggcatcattt gcaacattgg ctccgtcacc ggtttcaatg ccatctacca ggttcccgtt
     1441 tactccggct ccaaggcggc ggtggttaac tttacctcct ccctggcggt aagtaatcca
     1501 acgtttttcc tactccccaa aactaaatat ttctttgaaa ttccatagaa actggctccc
     1561 attactggtg tcactgctta cactgtcaat cctggcatca ctaagaccac tctggtccac
     1621 aaattcaact cgtggctgga tgtggagccc cgtgtggcgg agaagctgct cgagcatccc
     1681 acccagacct ctcagcagtg cgccgagaac tttgtgaagg ccattgagct gaacaagaac
     1741 ggtgccatct ggaagttgga tctgggcacc ttggagccca tcacatggac ccagcactgg
     1801 gactcgggca tctaaaccat cccagagact ctatgggaca tggcgtttta gcttttagtt
     1861 tctttttttc cactcaattg ttacgtatat acatacacat atggcaataa ggctgatttg
     1921 attctctttt atggaaggaa ccccgttttg aatatgataa tacaaaatta tatttgagaa
     1981 atttcaacaa aatcgataag agctctagct ctcttttaat taaaaaaaac tagataaaaa
     2041 gagcaatggt cagtggtggt cagtggtcgt tgtggcctct atcgatttcc acacaaaaac
     2101 tttactgttt agtaatagaa aagaacttga gaaaggcagc caaaagaatg tacgatctga
     2161 cgggtaagca tgtctgctat gtggctgact gcggtggcat tgcactggag accagcaagg
     2221 ttctcatgac caagaatata gcggtgagtg gtagagtggt gtagagagtg gagagtgttc
     2281 cgaagtggtt ctggttctta aacaagtctc tagtctctag tctcttggct gctgctggta
     2341 gctgtcgaaa ctaattaagt taatgacttt ttcatgtttt gtttttgtcc gctgactttt
     2401 gctgttcaaa gctacaatta agtttatgtt ttgagtttga tctacttttg cttaagggaa
     2461 gtactttcga ttacggattg ctggcatata aaaatagata ccaaggaatg tgcctcatat
     2521 catattgatt ctatcttgat tctttgccca gaaactggcc atccttcaga gcgtggaaaa
     2581 ccccccggcc atcgctcagt tacaatcgat taagcacagc acacagatct tcttctggac
     2641 cttcgatgtg accatggccc gcgaggagat gaagaagtac ttcgatgagg tcatggtcca
     2701 gatggactac atcgatgtgc tgatcaatgg ggccaccctg tgcgatgagc ggaacattga
     2761 tgccaccatc aatacgaatc tgaccggcat gatgaacacc gtggccactg tgctgcccta
     2821 tatggaccga aagatgggcg gttcgggtgg actgatcgtg aatgtcacct ccgtcatagg
     2881 actggatcca tcgccagtct tttgtgcgta cagtgcctca aagtttggtg tgattgggtt
     2941 cacccgaagt ctagcggtga gtgggcgaga tcttaacttg aaaatgctct ctaatgtctt
     3001 tcattctttc gcaggatccc ctgtattaca cccaaaatgg tgtggctgtc atggccgtct
     3061 gctgtggtcc caccaaagtg tttgtcgatc gggaactgac tgccttcctg gcatacggcc
     3121 aaacctttgc cgatcgcctg cgtcgtgccc cctgccagtc gacggccgtc tgcggccaga
     3181 atatagtaaa tgccatcgag agatcggaga acggacagat ctggattgcc gacaagggcg
     3241 gcctggagtc ggtggccctc cactggtatt ggcatatggc cgatcagttt gtgaactaca
     3301 tgcagagcac tgacgatgag gatcaggagt tttttttggg tcagcgatga ggcatgcctt
     3361 gatccacgga ataaaacatc aaatgtgtca caaaaagtag gcaacttgtt tgtgggtctg
     3421 gaaatagatt tgaaatattt tgaggtcata tgaataatga cataaatgag ggtgcctgaa
     3481 agtatgccaa gttt
//