From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Bacillus subtilis ORFR; ORFQ; transcriptional terminator (rho)
Date: 29 Dec 92 19:56:41 GMT
Distribution: bionet
Checksum: 56111 12

LOCUS       BACRHOTDKX   5522 bp ds-DNA             BCT       10-DEC-1992
DEFINITION  Bacillus subtilis ORFR; ORFQ; transcriptional terminator (rho)
            gene, 3' end; ribosomal protein L31; ORFP; thymidine kinase (tdk)
            gene, complete cds.
ACCESSION   M97678
KEYWORDS    rho gene; ribosomal protein L31; tdk gene; thymidine kinase;
            transcriptional terminator protein.
SOURCE      Bacillus subtilis (library: Transposon Tn insertional library) DNA.
  ORGANISM  Bacillus subtilis
            Prokaryotae; Firmicutes; Endospore-forming rods and cocci;
            Bacillaceae.
REFERENCE   1  (bases 1 to 5522)
  AUTHORS   Trach,K., Chapman,J.W., Piggot,P., LeCoq,D. and Hoch,J.A.
  TITLE     Complete sequence and transcriptional analysis of the spo0F region
            of the Bacillus subtilis chromosome
  JOURNAL   J. Bacteriol. 170, 4194-4208 (1988)
  STANDARD  full automatic
REFERENCE   2  (bases 1 to 5522)
  AUTHORS   Quirk,P.G., Dunkley,E.A.Jr., Lee,P. and Krulwich,T.A.
  TITLE     Identification of a Bacillus subtilis rho gene
  JOURNAL   J. Bacteriol. (1992) In press
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     RBS             255..259
                     /note="putative"
     terminator      1246..1267
     RBS             1625..1630
     terminator      2975..2996
     RBS             3031..3035
     terminator      3259..3289
     RBS             complement(4401..4405)
     RBS             4706..4710
     terminator      5295..5312
     CDS             <1..240
                     /function="unknown"
                     /citation=[2]
                     /citation=[1]
                     /note="putative;  this submission continues the sequence
                     published by Trach et al., and completes the cds
                     designated orfR by this group; ORFR"
                     /codon_start=1
                     /translation="SAIITGPVELQGAKVKASDLRAGACLVVAGLMADGVTEITGLEH
                     IDRGYSSLEKKLEGLGATIWRERMTDEEIEQLQNS"
     CDS             271..1236
                     /function="unknown"
                     /citation=[2]
                     /note="putative;  may form operon with orfR; ORFQ"
                     /codon_start=1
                     /translation="MERSLSMELVRVTEAAALASARWMGRGKKDEADEAATSAMRDVF
                     DTVPMKGTVVIGEGEMDEAPMLYIGEKLGNGYGPRVDVAVDPLEGTNILASGGWNALT
                     VIAVADHGTLLNAPDMYMQKIAVGPEAVGCIDIEAPVIDNLKAVAKAKNKDVEDVVAT
                     ILNRERHAKIISELREAGARIKLINDGDVAGAINTAFDHTGVDILFGSGGAPEGVLSA
                     VALKALGGEIIGKLLPQSEEEITRCHKMGLDLSKVLRMEDLVKGDDAIFAATGVTDGE
                     LLKGVQFKGSVGTTESLVIRAKSGTVRFVDGRHSLKKKPNLVIRP"
     CDS             1643..2926
                     /function="transcriptional terminator"
                     /standard_name="Rho factor"
                     /gene="rho"
                     /citation=[2]
                     /note="putative"
                     /codon_start=1
                     /translation="MKDVSISSLENMKLKELYELARHYKISYYSKLTKKELIFAILKA
                     NAEQEDLLFMEGVLEIIQSEGFGFLRPINYSPSSEDIYISASQIRRFDLRNGDKVSGK
                     VRPPKENERYYGLLHVEAVNGDDPESAKERVHFPALTPLYPDRQMVLETKPNFLSTRI
                     MDMMAPVGFGQRGLIVAPPKAGKTMLLKEIANSITANQPEAELIVLLIDERPEEVTDI
                     ERSVAGDVVSSTFDEVPENHIKVAELVLERAMRLVEHKKDVIILMDSITRLARAYNLV
                     IPPSGRTLSGGIDPAAFHRPKRFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEEF
                     KGTGNMELHLDRSLAERRIFPAIDIRRSGTRKEELLVPKEHLDRLWSIRKTMSDSPDF
                     AEKFMRKMKKTKTNQEFFDILNQEWKQANLSSARR"
     CDS             3045..3245
                     /gene="L31"
                     /citation=[2]
                     /note="putative"
                     /product="ribosomal protein"
                     /codon_start=1
                     /translation="MKAGIHPNFKKATVKCACGNEFETGSVKEEVRVEICSECHPFYT
                     GRQKFASADGRVDRFNKKYGLK"
     CDS             complement(3457..4395)
                     /function="unknown"
                     /citation=[2]
                     /note="ORFP; putative"
                     /codon_start=1
                     /translation="MPIVLVDWSDIREQKRLMVLRASVALHGRSVTLYEKAFPLSEQC
                     SKKAHDQFLADLASILPSNTTPLIVSDAGFKVPWYKSVEKLGWYWLSRVRGKVQYADL
                     GAENWKPISNLHDMSSSHSKTLGYKRLTKSNPISCQILLYKSRSKGRKNQRSTRTHCH
                     HPSPKIYSASAKEPWVLATNLPVEIRTPKQLVNIYSKRMQIEETFRDLKSPAYGLGLR
                     HSRTSSSERFDIMLLIALMLQLTCWLAGVHAQKQGWDKHFQANTVRNRNVLSTVRLGM
                     EVLRHSGYTITREDLLVAATLLAQNLFTHGYALGKL"
     CDS             4716..5258
                     /EC_number="2.7.1.21"
                     /gene="tdk"
                     /citation=[2]
                     /note="putative"
                     /product="thymidine kinase"
                     /codon_start=1
                     /translation="MCIHLNLMIFTKIIRGFISAKQEVRVFKPVIDNRYSEAAVVSHN
                     GTSMTSYAISSAADIWDHISESTDVVAVDEVQFFDQEIVEVLSSLADKGYRVIAAGLD
                     MDFRGEPFGVVPNIMAIAESVTKLQAVCSVCGSPASRTQRLIDGKPASYDDPVILVGA
                     AESYEARCRHHHEVPGKSKK"
BASE COUNT     1588 a   1115 c   1353 g   1466 t
ORIGIN      
        1 tctgccatca tcacaggtcc tgtcgagctt caaggcgcaa aagtgaaggc gagtgatctg
       61 cgtgccggag cctgcttggt ggtagccgga ctgatggctg atggcgtcac ggaaattacg
      121 ggactggagc atattgaccg aggatacagc agccttgaga agaagcttga ggggcttgga
      181 gcgacaattt ggcgtgaaag aatgactgac gaagaaatag aacagcttca aaattcataa
      241 tggtttcttg tgagaggaga tcgtgttgaa atggaaagaa gtttatcaat ggaattggta
      301 cgtgtgacag aagctgcggc attggcatca gcaagatgga tgggccgggg aaaaaaagat
      361 gaggctgatg aagcggcaac aagtgcaatg agagacgttt ttgatacagt gccgatgaaa
      421 gggaccgttg taatcggtga aggggaaatg gacgaagcgc caatgctgta tatcggggaa
      481 aaactcggaa acgggtacgg ccctcgcgtt gatgtcgcag ttgaccctct tgaaggcaca
      541 aacatcttag caagcggcgg ctggaacgcg ctgacggtta tcgcggtcgc agaccatggc
      601 acgcttctga atgctcctga catgtatatg caaaaaatcg cagtcggtcc ggaagcggtg
      661 ggctgcattg acatcgaagc acctgtcatt gacaacctta aagcggtggc aaaagcgaaa
      721 aacaaggacg ttgaggacgt cgttgccaca attttaaacc gtgaaagaca cgcaaaaatc
      781 atttccgagc ttcgtgaggc cggcgccaga atcaaattga tcaatgacgg cgatgtcgca
      841 ggcgccatca acacggcttt cgaccacacg ggcgttgata tcctgttcgg atcaggcggc
      901 gctcctgagg gtgtgctttc cgctgttgcg ctaaaagcac tcggcggaga aatcattggt
      961 aaacttcttc cgcaaagcga agaagaaatc actcgctgtc acaaaatggg ccttgattta
     1021 agcaaagtgc ttcgcatgga agaccttgtg aagggtgatg acgcgatttt tgcagctaca
     1081 ggggttactg acggcgagct gttaaaagga gttcaattca aaggctctgt cggtacaact
     1141 gaaagcttag ttatccgtgc aaaatcgggc actgttcgtt tcgttgacgg cagacacagc
     1201 ttgaaaaaga aaccaaatct tgtaatccgt ccataacaaa cagaaaaagc gggtgtgagc
     1261 ccgctttaac ttttgcagaa aagcgtcaac tctatcaatt ccgtgtcaaa accttatttc
     1321 agttgaataa ttttttgaaa taaggtaaaa ataaagagtg ctgcgaattt tactgcgtaa
     1381 taccttcttt taagcactag gaaagtttca atttctaccg catacagacg atataagtat
     1441 tgatcttctc atgtttttgt cctcgcttcc ttcaaaactc attaagacaa tccttttaac
     1501 aaaagcgccg atcatgggtg gaagtgttac cgatagaagt tcctttatgt taaaaaaaga
     1561 atgtgctttg gcggatgttg tgcaaaagca gcagatgaca tgttcagagt aagatattga
     1621 ctatgaaaag cgtggtgttt ttatgaaaga cgtatctatt tcctctttgg aaaatatgaa
     1681 attgaaagag ctttatgaac ttgcaagaca ttataaaatc tcctattaca gcaaactgac
     1741 aaaaaaagaa ctcattttcg ccattctgaa agcgaatgca gaacaggaag atctgctgtt
     1801 tatggaaggc gttctcgaga tcatccagtc tgaaggtttc ggattcctga gaccgatcaa
     1861 ctactctcca agctcagaag acatttacat ctcagcttca caaatccgcc gtttcgattt
     1921 gcggaacgga gacaaagtat ctggcaaggt tcgcccgcca aaagaaaatg agcgttacta
     1981 tggacttttg cacgttgaag cagtaaatgg ggatgatccc gaatctgcaa aagagcgtgt
     2041 gcatttcccg gctcttacgc cactttatcc ggatcgtcaa atggtgcttg aaacaaagcc
     2101 gaacttcttg tctacaagaa ttatggacat gatggcgccg gttggatttg ggcagcgcgg
     2161 attgattgtt gcgccgccga aagccggaaa aacgatgttg ctgaaggaaa ttgccaacag
     2221 cattacagcg aaccagcctg aagcagagct gatcgtgctt ttaattgacg aaagacctga
     2281 ggaagtaacc gatatcgagc gctctgtagc tggggatgtc gtcagctcaa cgtttgatga
     2341 agtgccggaa aaccatatca aagtggccga gcttgtgctt gaacgtgcga tgcgtctcgt
     2401 ggaacacaaa aaagacgtca ttatcctgat ggacagcatc acacgtcttg cccgcgccta
     2461 caacttagtg attccgccaa gtggaagaac gctttccggg gggattgacc cagcggcgtt
     2521 ccaccgtccg aaacgcttct ttggggctgc gagaaatatc gaagagggcg gcagcttaac
     2581 catccttgct acggctctgg tcgatacagg ttcacgtatg gatgatgtca tttatgaaga
     2641 attcaaggga acaggcaaca tggagctcca tcttgaccgc tctcttgccg agcgccgcat
     2701 cttccctgcc atcgatatcc gccgttcagg aacgcgcaaa gaagagctgc ttgtgcctaa
     2761 agagcatctt gatcgtttat ggtctatccg caaaacgatg tctgattcac ctgatttcgc
     2821 agaaaagttc atgagaaaaa tgaaaaaaac caaaacaaac caggaatttt tcgatattct
     2881 caatcaagaa tggaaacagg caaatctatc atctgcaaga aggtaaattg tatttgcaaa
     2941 agaagtaaat cactgatata attttctcat atgcgtttcg gacaatgctc cgaaataata
     3001 actctgtttc cacacgggat tcagggcgga aggagatgga tacaatgaaa gcaggaattc
     3061 atcctaattt caaaaaagca acagttaaat gcgcttgcgg aaatgaattt gaaacaggct
     3121 cagtaaaaga agaggtacgc gttgagattt gctctgaatg ccacccattc tacactggcc
     3181 gtcaaaaatt cgcttctgct gatggtcgtg ttgatcgctt taacaaaaaa tacggtctta
     3241 agtaataata gatttctcaa caggcaagca gcagtcttgc ctgtttttta tattgtctaa
     3301 aacacagctg gcagagagag gggagcgaag cctatgtaca taatgaaaca aagcgggtgg
     3361 cttgagctga tttgcggcag catgttctcg gggaaatctg aagagctgat cagaagagtg
     3421 aagagagcaa cttatgccaa gcactgagag atcccctcat aatttcccca aagcgtaacc
     3481 atgtgtgaat aaattttgag ctagtagggt tgcagccacg agtaagtctt cccttgttat
     3541 tgtgtagcca gaatgccgca aaacttccat gcctaagcga actgttgaga gtacgtttcg
     3601 atttctgact gtgttagcct ggaagtgctt gtcccaacct tgtttctgag catgaacgcc
     3661 cgcaagccaa catgttagtt gaagcatcag ggcgattagc agcatgatat caaaacgctc
     3721 tgagctgctc gttcggctat ggcgtaggcc tagtccgtag gcaggacttt tcaagtctcg
     3781 gaaggtttct tcaatctgca ttcgcttcga atagatatta acaagttgtt tgggtgttcg
     3841 aatttcaaca ggtaagttag ttgctagaac ccatggctcc tttgccgacg ctgagtagat
     3901 tttaggtgac gggtggtgac aatgagtccg tgtcgagcgc tgattttttc ggcctttaga
     3961 gcgagattta tacaatagaa tttggcatga gattggattg cttttagtca gcctcttata
     4021 gcctaaagtc tttgagtgac tagatgacat atcatgtaag ttgctgatag gtttccagtt
     4081 ttccgctcct aggtctgcat attgtacttt tcctcttact cgacttaacc agtaccaacc
     4141 cagcttctca acggatttat accatggcac tttaaagcca gcatcactga caatgagcgg
     4201 tgtggtgtta ctcggtagaa tgctcgcaag gtcggctaga aattggtcat gagctttctt
     4261 tgaacattgc tctgaaagcg ggaacgcttt ctcataaaga gtaacagaac gaccgtgtag
     4321 tgcgactgaa gctcgcaata ccataagtcg tttttgctca cgaatatcag accagtcaac
     4381 aagtactatg ggcatcgtat tctccgacag ataaagctag catgccagcg gtatacagcg
     4441 agtcgctctt tgtggaggtg acgattacct aacaatcggt cgattcgttt gatgttatgt
     4501 tttgttctcg ctttggttgg caggttacgg ccaagttcgg taagagtgag agttttacag
     4561 ccaagtaatg cgtggcaagc caacgtaaag ctgttgagtc gttttaagtg taattcgggg
     4621 cagaattggt aaagagagtc gtgtaaaata tcgagttcgc acatcttgtt gtctgattat
     4681 tgatttttcg cgaaaccatt tgatcatgtg acaagatgtg tatccacctt aacttaatga
     4741 tttttaccaa aatcattagg ggattcatca gtgccaagca ggaagtcagg gtatttaagc
     4801 ctgtaattga taaccgatac agcgaagctg ctgttgtctc ccataatgga acatcgatga
     4861 cgagctatgc catttcgtct gctgcggaca tttgggatca catcagtgaa agtacagatg
     4921 tggttgcggt tgatgaagtg cagttttttg atcaggaaat tgttgaggtt ttatcatctc
     4981 ttgccgataa aggctaccgt gtgatagcag caggccttga tatggatttt aggggagagc
     5041 cgttcggtgt cgtcccgaat atcatggcga ttgcggaaag tgtgacaaag ctgcaagccg
     5101 tctgttctgt ttgcggatca ccggcgagca gaacacagcg cctcattgac ggcaaacctg
     5161 cttcttacga tgatccggtc attttggttg gtgcagctga atcatatgaa gcaagatgca
     5221 gacatcatca cgaagtccca ggtaaatcta aaaaatagtc caaaacatgg gtataaagat
     5281 agaatgcacc ggctaaaaac accggtgttt tttttatatt ctgataatat tcaattaaaa
     5341 aatttaaata agtatattaa aaaattaagt aattttcttc tcttgaattt tgattttgac
     5401 aggcatacaa tagcttacgt aatgtaaata gttttgagac attgttcgaa taattgtcac
     5461 aaagtcgcat aaagatcatg atcggtcata ttttagatcc gttattactt aacttggtta
     5521 ac
//