From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: E.coli CFA/I fimbrial operon cfa a, cfa b, cfa c, cfa e, cfa d'
Date: 4 Sep 92 10:03:08 GMT
Distribution: bionet
Checksum: 05067    16

LOCUS       ECOCFAIA     7176 bp ds-DNA             BCT       04-SEP-1992
DEFINITION  E.coli CFA/I fimbrial operon cfa a, cfa b, cfa c, cfa e, cfa d'
            genes, complete cds.
ACCESSION   M55661
KEYWORDS    colonisation factor antigen I; regulatory protein.
SOURCE      Escherichia coli (enterotoxigenic) DNA.
  ORGANISM  Escherichia coli
            Prokaryotae; Gram-negative facultatively anaerobic rods;
            Enterobacteriaceae.
REFERENCE   1  (bases 1 to 7176)
  AUTHORS   Hamers,A.M., Pel,H.J., Willshaw,G.A., Kusters,J.G.,
            van der Zeijst,B.A. and Gaastra,W.
  TITLE     The nucleotide sequence of the first two genes of the CFA/I
            fimbrial operon of human enterotoxigenic Escherichia coli
  JOURNAL   Microb. Pathog. 6, 297-309 (1989)
  STANDARD  full staff_entry
REFERENCE   2  (bases 1 to 7176)
  AUTHORS   Gaastra,W., Jordi,B.J., Mul,E.M., Hamers,A.M., McConnell,M.M.,
            Willshaw,G.A., Smith,H.R. and van der Zeijst,B.A.
  TITLE     A silent regulatory gene cfaD' on region 1 of the CFA/I plasmid NTP
            113 of enterotoxigenic Escherichia coli
  JOURNAL   Unpublished (1990)
  STANDARD  full staff_entry
REFERENCE   3  (sites)
  AUTHORS   Jordi,B.J., Willshaw,G.A., Van der Zeijst,B.A. and Gaastra,W.
  TITLE     The complete nucleotide sequence of region 1 of the CFA/I fimbrial
            operon of human enterotoxigenic Escherichia coli
  JOURNAL   DNA Sequence 2, 257-263 (1992)
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     sig_peptide     848..904
                     /gene="cfa a"
                     /codon_start=1
     CDS             848..1468
                     /product="colonisation factor antigen a"
                     /gene="cfa a"
                     /codon_start=1
                     /translation="MHKLFYLLSLLMAPFVANANFMIYPISKDLKNGNSELVRVYSKS
                     KEIQYIKIYTKKIINPGTTEEYKVDIPNWDGGLVVTPQKVILPAGASKSIRLTQFKIP
                     KKEEVYRVYFEAVKPDSKENVIDNKKLTTELSVNIIYAALIRSLPSEQNISLNISRNA
                     KKNIIIYNNGNVRAGVKDIYFCKSSNIDDNCVKKAYNKNIYPEKVI"
     mat_peptide     905..1465
                     /product="colonisation factor antigen a"
                     /gene="cfa a"
                     /codon_start=1
     CDS             1595..2107
                     /product="colonisation factor antigen b"
                     /gene="cfa b"
                     /codon_start=1
                     /translation="MKFKKTIGAMALTTMFVAVSASAVEKNITVTASVDPAIDLLQAD
                     GNALPSAVKLAYSPASKTFESYRVMTQVHTNDATKKVIVKLADTPQLTDVLNSTVQMP
                     ISVSWGGQVLSTTAKEFEAAALGYSASGVNGVSSSQELVISAAPKTAGTAPTAGNYSG
                     VVSLVMTLGS"
     sig_peptide     1595..1663
                     /gene="cfa b"
                     /codon_start=1
     mat_peptide     1664..2104
                     /product="colonisation factor antigen b"
                     /gene="cfa b"
                     /codon_start=1
     CDS             2172..4781
                     /product="colonisation factor antigen c"
                     /gene="cfa c"
                     /codon_start=1
                     /translation="MKHKKKNRLVVAISVALIPYIGVTGDIPDSFRDLWGEQDEFYEV
                     KLYGQTLGIHRIKTTPTHIKFYSPESILDKINLKKEKEKELSVFFTNSFSRNGNMSCQ
                     GNTTIQYNCNYIKTKSVDVIVDDVDNVVNLFIGNEFLDSEAHNDEYHQLSRNVKKAFI
                     QSQTINLSDSGKYKRLSISGNSALGITDTSYAVLNWWMNYNKSNGYSNNEKTINSLYF
                     RHDLDKRYYYQFGRMDRTDLSQSISGSFNFNLLPLPDIDGIRTGTTQSYIKNTDKFIA
                     SPVTVMLTNFSRVEAFRNDQLLGVWYLDSGVNELDTARLPYGSYDLKLKIFENTQLVR
                     EEIIPFNKGRSSIGDMQWDIFVQGGNIVNDNDRYIEKQNNHKSSINTGLRLPITKNIS
                     VQQGVSVIDNKSYYEGSLKWNSGILSGSLNSEFSFLWGDNAKGNYQSISYTDGFSLSF
                     YHNDKRVDNCGRNYNAGWSGCYESYSASLSIPLLGWTSTLGYSDTYSESVYKSHILSE
                     YGFYNQNIYKGRTQRWQLTSSTSLKWMDYNFMPAIGIYNSEQRQLTDKGGYISVTITR
                     ASRENSLNTGYSYNYSRGNYSSNELFVDGYMTSTNNGDYHEAGMRFNKNRHNAEGRLS
                     GRINNRFGDLNGSFSMNKNRNTNSTNHSLTGGYNSSFALTSDGFYWGGSTAGLTKLAG
                     GIIKVKSNDTKKNLVKVTGTLYGDYSLGSNDNAFIPVPALTPASLIIEDNNYGDNNIS
                     ILAPTNNDMFMLPGNVYPVEIETKVSVSYIGRGFDPNGTPLSGAHVLNEPHVILDEDG
                     GFSFEYTGNEKTLFLLKGRTIYTCQLGKNKVHKGIVFVGDVICDINSTSSLPDEFVKN
                     PRVQDLLAKNDKG"
     sig_peptide     2172..2237
                     /gene="cfa c"
                     /codon_start=1
     mat_peptide     2238..4778
                     /product="colonisation factor antigen c"
                     /gene="cfa c"
                     /codon_start=1
     CDS             4785..5867
                     /note="unpublished sequence"
                     /product="colonisation factor antigen e"
                     /gene="cfa e"
                     /codon_start=1
                     /translation="MNKILFIFTLFFSSGFFTFAVSADKNPGSENMTNTIGPHDRGGS
                     SPIYNILNSYLTAYNGSHHLYDRMSFLCLSSQNTLNGACPSSDAPGTATIDGETNITL
                     QFTEKRSLIKRELQIKGYKQFLFKNANCPSKLALNSSHFQCNREQASGATLSLYIPAG
                     ELNKLPFGGVWNAVLKLNVKRRYDTTYGTYTINITVNLTDKGNIQIWLPQFKSNARVD
                     LNLRPTGGGTYIGRNSVDMCFYDGYSTNSSSLEIRFQDDNSKSDGKFYLKKINDDSKE
                     LVYTLSLLLAGKNLTPTNGQALNINTASLETNWNRITAVTMPEISVPVLCWPGRLQLD
                     AKVKNPEAGQYMGNIKITFTPSSQTL"
     sig_peptide     4785..4844
                     /gene="cfa e"
                     /codon_start=1
     mat_peptide     4845..5864
                     /product="colonisation factor antigen e"
                     /gene="cfa e"
                     /codon_start=1
     CDS             complement(6390..6826)
                     /note="truncated CfaD-like protein with a stop codon and
                     frameshift deletions between bp 6029 and 6824."
                     /product="colonisation factor antigen d'"
                     /gene="cfa d'"
                     /codon_start=3
                     /translation="MDFKYTEEKEMIKINNIMIHIYTVLYTSNCIMDIYSEEEKITCS
                     SNRLVFLERGVNISVRIQKQILSEKPYIAFRLNGDILRHLKNALMIIYGMSKIDINDC
                     RNMSRKIIKTEVDKTLLDVLKNINSYDDSVFISSLIYLISKI"
BASE COUNT     2442 a   1129 c   1271 g   2333 t      1 others
ORIGIN
        1 cttctctgct ctgrctgaag gtctgcagcc tcatcaactg agtgataaac aatcaactaa
       61 aggagcactt atgagcgatg tattgttgtc tatggatacc tgtcaccagc tgctgcggat
      121 gctgaaaaca cgccagtcag agtagttgac gtctaatagt taggggttca gatgagtggc
      181 tcagcgtatg taccatttgc ttccatacgg atttaaacct caggcggaat aggtgataga
      241 ttgtaaatct attcgtcatt atgttaactc aatttcaata tgcaaggcgg catagtgtgg
      301 tgtcattttg ttaaaaactt tttaactttg tcgattttag caggccacaa acgacaaacc
      361 ttcccgaagc cggtacccta gtgcttttcc tgataaatcg cctgtcagac tgatgtacac
      421 ccaacctaac ggtgacatta agcattaatc ttcacgccta aaccttatgt gagaacggcg
      481 ttgtacaacc ttgtagtggc gatgagcatt acaacgaaaa ttatccacat agtgcgttgg
      541 gatatttctt attgaggaaa tatcggtgtc attaagtaac gttaacttaa gataaagaat
      601 ctgtctggaa atcgcagggc caagaacact cagtacatct gtggcgataa tactatcgct
      661 tcttattcat tccaatatgc agttcctgtg gatatttgct tgaacatcgc agcattagtg
      721 agtgcacaga attatagcat cgataccaaa taacacaggt tacacaatga tttgtttgca
      781 ttgtcttgtt tttttatctc attttttttt gttttgattt attattgatg gaagctcagg
      841 aggaaatatg cataaattat tctatttact aagtttactc atggctccat ttgttgcaaa
      901 tgcaaacttt atgatatatc caatatcaaa agatttaaag aatggaaata gcgagttagt
      961 tcgtgtttat tcaaaatcaa aagagataca atatataaaa atatatacaa agaagattat
     1021 taatcccggt acaactgaag aatataaggt tgatataccc aattgggatg gtgggcttgt
     1081 agtcactcct cagaaagtta tcctccctgc aggagcgagt aaatcaatac gtttaactca
     1141 atttaaaata ccaaaaaaag aggaagttta cagagtatat tttgaggcgg taaaaccaga
     1201 tagcaaagaa aatgtaattg ataataaaaa actaacaaca gagctatctg ttaatataat
     1261 ttatgcggct ctaattagat ctttaccaag tgaacaaaat atatcactaa atatttctag
     1321 aaatgcaaaa aaaaatataa ttatttataa taatgggaat gttagagcag gcgttaaaga
     1381 tatttatttt tgtaagtcat ctaatatcga tgataactgt gtaaaaaaag cgtataacaa
     1441 gaatatatat ccagaaaaag tcatttgata cgctggttaa taacaatttt ttcttatgtt
     1501 ttcattaaat taaaccatga aggcatagaa aaagagcaag ggctaataca attaaaggtt
     1561 ccttgattac tcatctatat actaaggagt tctaatgaaa tttaaaaaaa ctattggtgc
     1621 aatggctctg accacaatgt ttgtagcagt gagtgcttca gcagtagaga aaaatattac
     1681 tgtaacagct agtgttgatc ctgcaattga tcttttgcaa gctgatggca atgctctgcc
     1741 atcagctgta aagttagctt attctcccgc atcaaaaact tttgaaagtt acagagtaat
     1801 gactcaagta catacaaacg atgcaactaa aaaagtaatt gttaaacttg ctgatacacc
     1861 acagcttaca gatgttctga attcaactgt tcaaatgcct atcagtgtgt catggggagg
     1921 acaagtatta tctacaacag ccaaagaatt tgaagctgct gctttgggat attctgcatc
     1981 cggtgtaaat ggcgtatcat cttctcaaga gttagtaatt agcgctgcac ctaaaactgc
     2041 cggtaccgcc ccaactgcag gaaactattc aggagtagta tctcttgtaa tgactttggg
     2101 atcctgattc ttttatttaa aaggaggggg tatgtttata catccctcct ttttcatgga
     2161 aatgaattta tatgaagcat aaaaaaaaga atagattagt cgtagcgatt tcagttgcat
     2221 taattccata tataggggtt accggagata tacccgactc tttccgtgat ttatggggag
     2281 aacaagatga attttatgaa gtaaaactat atgggcaaac tctaggaata catcgaatta
     2341 aaacaacccc aacacatatt aagttttatt cacccgaaag cattttagat aaaataaatt
     2401 taaaaaaaga aaaggaaaag gaattgagtg ttttttttac taattctttt tcaagaaatg
     2461 gcaatatgag ttgtcagggt aacactacta tacagtataa ctgcaattac attaaaacaa
     2521 aatcagtaga tgtcatcgtt gatgatgttg ataatgttgt taaccttttt ataggtaatg
     2581 aatttctgga ttctgaagca cacaatgatg aatatcatca attatcacgg aatgtaaaaa
     2641 aagcttttat acaaagccag acaattaatc tctcagattc tggaaagtat aaaagattgt
     2701 ctatttcagg gaatagcgcg ctgggtatta cagatacaag ttatgctgtc ttaaattggt
     2761 ggatgaatta caataaatct aatggttaca gcaacaacga aaaaacaatc aatagtttat
     2821 actttagaca tgatttagat aagagatatt attatcaatt tggacgaatg gatcgtacag
     2881 atttatcaca aagtattagc gggagcttta attttaactt acttccttta cccgatattg
     2941 atggtatacg gacaggaacc acacaatctt atatcaaaaa tacagataag tttatcgcat
     3001 cccctgtaac tgttatgtta actaattttt ccagagtgga agcttttcgc aatgatcaat
     3061 tattgggcgt atggtattta gattctggag taaatgaatt agatacagct cgtttacctt
     3121 atggcagtta cgatcttaaa ttaaaaattt ttgaaaacac tcaattagtt cgtgaagaaa
     3181 taattccttt taataaagga agaagctcta ttggtgatat gcaatgggat attttcgttc
     3241 agggagggaa tattgttaat gataatgatc gttacataga aaaacaaaat aatcataagt
     3301 catcgattaa tactgggcta cgtttaccaa ttacgaaaaa tatctctgtt caacagggag
     3361 tatctgttat agataataaa agttattatg aaggaagtct gaaatggaat tccggcattc
     3421 tatctggctc actaaatagt gagttcagtt ttctttgggg agataatgca aaaggtaatt
     3481 atcaaagtat ctcgtatacc gatggattta gcttatcatt ttatcataat gataagcggg
     3541 tcgataattg tggaagaaat tacaatgctg gttggagtgg atgctacgaa tcatattcgg
     3601 catctttaag tattccttta ttaggatgga caagtactct gggatatagt gacacttata
     3661 gtgaatcagt gtataaaagc catattcttt ctgaatatgg cttttataat caaaacatat
     3721 ataaagggag aacccaaaga tggcaactga cttcatccac ctctttaaaa tggatggatt
     3781 ataattttat gccagcaatt ggaatatata acagtgaaca aagacaactg actgataaag
     3841 gcggatatat atctgtaact atcacccgag ccagcagaga aaattcatta aatacagggt
     3901 attcttacaa ctattccaga ggaaactatt cttctaacga attatttgtt gatggatata
     3961 tgacatcaac aaataatggt gattatcatg aggcaggaat gcgttttaat aaaaatagac
     4021 ataatgcaga aggtagactt tcaggtcgta taaacaatcg atttggagat ttaaatggtt
     4081 cattcagcat gaataaaaac agaaacacca acagtaccaa tcattctctc actggtggtt
     4141 ataattcctc atttgctctt acaagtgatg gattttactg gggaggaagt acagctggtt
     4201 tgacaaaact ggctggcggt attatcaagg ttaaatcaaa cgatactaaa aaaaacttgg
     4261 taaaagtgac tgggacattg tacggtgatt attcgctagg gagcaacgat aatgctttta
     4321 ttcctgtacc agcattaact ccagccagtt taatcattga agataataat tatggtgata
     4381 ataatatttc tatacttgcg ccaacaaaca acgatatgtt tatgttgccg ggtaatgttt
     4441 atcctgttga aattgaaacc aaagtaagtg tttcttatat tggtagaggt tttgacccaa
     4501 acggcacgcc actttctggc gcacatgttt tgaatgaacc acatgttatc ctggatgagg
     4561 acggtggatt ttcgtttgaa tatacaggta atgagaaaac acttttttta ttaaagggca
     4621 ggactattta tacatgtcaa ctggggaaaa ataaagttca taaaggcatt gttttcgtcg
     4681 gggatgttat atgtgatatt aatagcacaa gttccttacc agatgaattt gtaaagaacc
     4741 cacgtgtgca ggatttgctg gcaaagaatg ataaaggata aacgatgaat aaaattttat
     4801 ttatttttac attgtttttt tcttcagggt tttttacatt tgccgtatcg gcagataaaa
     4861 atcccggaag tgaaaacatg actaatacta ttggtcccca tgacaggggg ggatcttccc
     4921 ccatatataa tatcttaaat tcctatctta cagcatacaa tggaagccat catctgtatg
     4981 ataggatgag ttttttatgt ttgtcttctc aaaatacact gaatggagca tgcccaagca
     5041 gtgatgcccc tggcactgct acaattgatg gcgaaacaaa tataacatta caatttacgg
     5101 aaaaaagaag tctaattaaa agagaactgc aaattaaagg ctataaacaa tttttgttca
     5161 aaaatgctaa ttgcccatct aaactagcac ttaactcatc tcattttcaa tgtaatagag
     5221 aacaagcttc aggtgctact ttatcgttat acataccagc tggtgaatta aataaattac
     5281 cttttggggg ggtctggaat gccgttctga agctaaatgt aaaaagacga tatgatacaa
     5341 cctatgggac ttacactata aacatcacag ttaatttaac tgataaggga aatattcaga
     5401 tatggttacc acagttcaaa agtaacgctc gtgtcgatct taacttgcgt ccaactggtg
     5461 gtggtacata tatcggaaga aattctgttg atatgtgctt ttatgatgga tatagtacta
     5521 acagcagctc tttggagata agatttcagg atgataattc taaatctgat ggaaaatttt
     5581 atctaaagaa aataaatgat gactccaaag aacttgtata cactttgtca cttctcctgg
     5641 caggtaaaaa tttaacacca acaaatggac aggcattaaa tattaacact gcttctctgg
     5701 aaacaaactg gaatagaatt acagctgtca ccatgccaga aatcagtgtt ccggtgttgt
     5761 gttggcctgg acgtttgcaa ttggatgcaa aagtgaaaaa tcccgaggct ggacaatata
     5821 tggggaatat taaaattact ttcacaccaa gtagtcaaac actctagata acaacatatt
     5881 ggcgctattg ccggccaata ttgtaaatag gtaatctgtt tgttaacaaa acattttatt
     5941 tcaattcagt ttgcgtcgca ataaatctct actagagaca tttttataca gcatagtatt
     6001 atgcaacaca ttcaaaataa agtaattttt atcctccttt aaaataagta aaaaactgct
     6061 ttggtgcaac accataatgt ttattaaaaa ccctaataaa ataagatata ctagaaattc
     6121 caatcatatt tgatatctgt gatatctggt atggattttc aagcagcaat aacgcagcct
     6181 tgctcattct cgattgcatt aagatctggt taaaattagt attctcagat tctagtcttt
     6241 tcctgatggt tatttctgat acattaaacg tatctgcaat aatagccaaa gtccattttc
     6301 tggatagatc tttttcgata acactcctga ccttatcaga aaaaaattca cagatgatat
     6361 ataaattgat tctattattt ttcattattc taaatttttg aaatcaaata tatcagacta
     6421 gatataaaaa ctgagtcatc ataactattt atatttttta atacatccag taaggtttta
     6481 tctacttctg ttttaattat ttttcttgac atatttctac aatcattgat atctattttt
     6541 gacatgccat atattatcat taatgcattc tttaaatgtc ttagtatgtc tccgttcaat
     6601 ctgaatgcaa tatatggttt ttctgataaa atttgcttct gtattcttac agatatattt
     6661 actcctcttt caagaaatac aagtctgtta ctagaacatg tgattttctc ttcttctgaa
     6721 tatatatcca taatacaatt tgatgtatat aatacagtgt atatatgaat cataatatta
     6781 ttaattttta tcatttcttt ttcttcagtg tatttaaaat ccatcttata cctacaaaag
     6841 gcaagaggtt tgatttgctt gtacaatcac cttttgaaca gttggcctaa cttttgctaa
     6901 aagaaagata tgttttttca acttttttgc tttttttatt attgtatctt tttgatgctt
     6961 ttctgtgatg tacacttatg tgtgattaat gttttatcgg agatagtgtt tcaatggaaa
     7021 taaaagagtg ttttcatgtt ttatcaaaaa aagccgctct ggcagaaaaa aacatggatt
     7081 tagtttctgc atttgaactt tggaaacaag catcactttt gtgtaaaaac gctaaaaata
     7141 tagactggtg cataaataga gcaatgtttt gtgaag
//