From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human Ig germline kappa chain V-region pseudogene (HK100), 5' end,
Date: 12 Sep 92 10:02:39 GMT
Distribution: bionet
Checksum: 03204     4

LOCUS       HUMIGKVC      632 bp ds-DNA             PRI       12-SEP-1992
DEFINITION  Human Ig germline kappa chain V-region pseudogene (HK100), 5' end,
            clone HK100/498.
ACCESSION   J00250 J00251
KEYWORDS    constant region; germline; immunoglobulin light chain;
            kappa-immunoglobulin; pseudogene; variable region.
SOURCE      Homo sapiens (library: Lawn et al) fetus liver DNA.
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 68 to 632)
  AUTHORS   Bentley,D.L. and Rabbitts,T.H.
  TITLE     Human immunoglobulin variable region genes - DNA sequences of two V
            kappa genes and a pseudogene
  JOURNAL   Nature 288, 730-733 (1980)
  STANDARD  full staff_review
REFERENCE   2  (bases 1 to 139)
  AUTHORS   Bentley,D.L., Farrell,P.J. and Rabbitts,T.H.
  TITLE     Unrearranged immunoglobulin variable region genes have a functional
            promoter
  JOURNAL   Nucleic Acids Res. 10, 1841-1856 (1982)
  STANDARD  full staff_review
COMMENT     Also reported [1] are V-kappa genes HK101 and HK102. [1] states
            that this pseudogene is 78% homologous to the V-kappa gene HK101.
            [2] shows that this gene cannot be transcribed in vitro or in vivo.
            All sites are relative to Ig V-kappa (HK101) homolog. [1] compared
            with NBRF data.
FEATURES             Location/Qualifiers
     CDS             join(131..184,304..606)
                     /pseudo
                     /gene="IGKVP1"
                     /codon_start=1
     sig_peptide     join(131..184,304..315)
                     /gene="IGKVP1"
                     /codon_start=1
     intron          185..303
                     /gene="IGKVP1"
                     /note="putative"
     exon            304..606
                     /number=2
                     /gene="IGKVP1"
     mat_peptide     316..606
                     /pseudo
                     /gene="IGKVP1"
                     /codon_start=1
                     /partial
     iDNA            (606.608)..>632
                     /note="V-J intervening DNA"
                     /gene="IGKVP1"
     misc_signal     608..632
                     /note="putative recombination recognition signal
                     (incomplete)"
                     /note="putative"
BASE COUNT      171 a    154 c    136 g    171 t
ORIGIN      630 bp upstream of AluI site [Nature 288, 730-733 (1980)]
        1 attatgcagg taaagtcatt cttgcatctg ttgaaatttt cattttcaaa aaaacacagc
       61 caacttcctg aagacttgta cacaggctgg tcataccact tgcaagagtc agtcccagtc
      121 aagacacagc atggacatga gggtccctgc tcagctcctg gggctcctgc tgctctggct
      181 ctcaggcaag gaaggagaac actaggaatt tactgagcca gtgtggtcag tactacctgg
      241 ctattcagga aagtcctctc ataacatggt taatagtgtt aatagttttg tttcccatct
      301 caggtgccag atatgacatg cagatgaccc agtctccatc ctcgctgtct tcctgtctag
      361 gtgagagtca ccatcacatg ccaggaaagt caggcagggc attagccatg ttttagcctg
      421 atacaaagag aagccaggga aagcttctga gctcctgatc tacgatgcat ccaatttgca
      481 aacctgggtc ccattgcagt tatgtggcat tggatccagg acagatttga ttctcaccat
      541 tagcatcctc cagtctgaag ttgctgcaac ttcttattat tggtcaacag tataaaagtg
      601 accctctcac agtgttacaa acccaataag ct
//