From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human cellular transforming c-Ki-ras2 proto-oncogene, exon 4a.
Date: 31 Jul 92 10:01:37 GMT
Distribution: bionet
Checksum: 05516     4

LOCUS       HUMRASK05     866 bp ds-DNA             PRI       31-JUL-1992
DEFINITION  Human cellular transforming c-Ki-ras2 proto-oncogene, exon 4a.
ACCESSION   L00048 K00652 K00653
KEYWORDS    K-ras oncogene; c-myc oncogene; proto-oncogene; ras oncogene;
            transforming gene.
SEGMENT     5 of 6
SOURCE      Homo sapiens (library: Lawn et al.) lung DNA; and Homo sapiens
            colon and lung cDNA to mRNA.
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 600 to 719)
  AUTHORS   Shimizu,K., Birnbaum,D., Ruley,M.A., Fasano,O., Suard,Y.,
            Edlund,L., Taparowsky,E., Goldfarb,M. and Wigler,M.
  TITLE     Structure of the Ki-ras gene of the human lung carcinoma cell line
            Calu-1
  JOURNAL   Nature 304, 497-500 (1983)
  STANDARD  full staff_review
REFERENCE   2  (bases 1 to 866)
  AUTHORS   McGrath,J.P., Capon,D.J., Smith,D.H., Chen,E.Y., Seeburg,P.H.,
            Goeddel,D.V. and Levinson,A.D.
  TITLE     Structure and organization of the human Ki-ras proto-oncogene and a
            related processed pseudogene
  JOURNAL   Nature 304, 501-506 (1983)
  STANDARD  full staff_review
REFERENCE   3  (bases 600 to 724)
  AUTHORS   Capon,D.J., Seeburg,P.H., McGrath,J.P., Hayflick,J.S., Edman,U.,
            Levinson,A.D. and Goeddel,D.V.
  TITLE     Activation of Ki-ras2 gene in human colon and lung carcinomas by
            two different point mutations
  JOURNAL   Nature 304, 507-513 (1983)
  STANDARD  full staff_review
COMMENT     Approximately 1% of the mRNAs generated from this gene have the
            exon 4a variant stucture containing the sequence 0-1-2-3-4a-4b with
            exon 4b spliced onto 4a 5 bp after the exon 4a stop codon. The
            other 99% of the mRNAs generated have no exon 4a in their sequence;
            exon 4b is spliced directly onto exon 3.
            See comment for segment 1 of this entry.
            Complete source information:
            Human DNA (genomic library of Lawn et al.) [2]; human cDNA to colon
            (SW480) and lung (Calu-1) carcinoma cell lines mRNA, multiple
            clones [3]; human lung carcinoma cell line Calu-1 DNA [1]
FEATURES             Location/Qualifiers
     intron          order(L00047:459..>615,<1..599)
                     /number=D
                     /note="9.1 kb gap"
                     /gene="c-Ki-ras2"
     exon            600..723
                     /number=4a
                     /gene="c-Ki-ras2"
BASE COUNT      301 a    143 c    138 g    284 t
ORIGIN
        1 acagaagacc cagtctcagc ttcacttgta taccctggaa atagactgaa aggtgttaaa
       61 atttaaaata aaactcaagg ttccagtttc ttgactcacc tttgagattc ttttatgttt
      121 ttgttgtttt ttaacaaagg tttcacgtcc atattttacc atttttcttc tcattctccc
      181 ctggaggagg gtgtgggaat cgatagtata taaatcactt ttttcctaag tcaaagaagt
      241 aatttaaagc taacttcagt ttaggcttta attccaggac tagcaaacta aaatggttgc
      301 attaattgac aaacagatgc taatacctgt gtttaggctt gtcataatct ctcctaattc
      361 ctaatttaaa aattttaaaa tttaattcca ttagaaaaca aaactgactt ttaagaacaa
      421 accaggattc tagcccatat tttaaaactg catcctcagt tttattcaaa cagtctgatg
      481 tctgtttaaa aaaaaaaaaa tctcaagctc ataatctcaa acttcttgca catggctttc
      541 ccagtaaatt actcttacca atgcaacaga ctttaaagaa gttgtgtttt acaatgcaga
      601 gagtggagga tgctttttat acattggtga gagagatccg acaatacaga ttgaaaaaaa
      661 tcagcaaaga agaaaagact cctggctgtg tgaaaattaa aaaatgcatt ataatgtaat
      721 ctggtaagtt taagttcagc acattaattt tggcagaaag cagatgtctt ttaaaggtaa
      781 caaggtggca accactttag aactacttag gtgtagtatt ctaacttgaa gtattaaaag
      841 ataagaaact tgtttccata attagt
//