From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: S.marcescens ssb gene for single-stranded DNA-binding protein (SSB)
Date: 22 Jul 92 10:03:41 GMT
Distribution: bionet
Checksum: 38756     4

LOCUS       SMASSB       1193 bp ds-DNA             BCT       22-JUL-1992
DEFINITION  S.marcescens ssb gene for single-stranded DNA-binding protein (SSB)
ACCESSION   X65080
KEYWORDS    single stranded DNA binding protein; ssb gene.
SOURCE      Serratia marcescens DNA.
  ORGANISM  Serratia marcescens
            Prokaryotae; Gram-negative facultatively anaerobic rods;
            Enterobacteriaceae.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   de Vries,J.E. and Wackernagel,W.
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
COMMENT     *source: strain=Sr 41; *source: cell_line=E.coli DH5; *source:
            clone_library=cosmid gene bank;
            From EMBL    entry SMSSB;  dated 26-MAR-1992.
FEATURES             Location/Qualifiers
     CDS             complement(<1..222)
                     /gene="uvrA"
                     /codon_start=1
                     /translation="MDNIEVRGARTHNLKNINLIIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVD"
     promoter        complement(326..354)
                     /note="uvrA"
     misc_feature    complement(344..360)
                     /note="SOS-box (LEXA-binding site)"
     -35_signal      362..388
                     /note="ssbPI"
     -10_signal      437..468
                     /note="ssbPN1"
     -10_signal      449..454
                     /note="ssbPN2"
     CDS             500..1030
                     /product="single stranded DNA binding protein"
                     /gene="ssb"
                     /codon_start=1
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEQKEKTEWHRVVLFGKLAEVAGEYLRKGSQVYIEGSLQTRKWQDQSGQDRYTTEI
                     VVNVGGTMQMLGGRQGGGAPAGQSAGGQGGWGQPQQPQGGNQFSGGQQQSRPAQNSAP
                     ATSNEPPMDFDDDIPF"
BASE COUNT      290 a    315 c    353 g    235 t
ORIGIN
        1 gtcgacgtcc ggtttttcca tcagcgacag gaactggcgc gcataggcgg agagtgattc
       61 gacatagcgc cgctgccctt cggcatacag cgtatcgaaa gccagcgaag acttgccgga
      121 accggacagg ccggtgacga cgatcagttt gtcacgcggg atgatcaggt tgatgttttt
      181 cagattatgg gttcgagcac cacgaacttc gatattgtcc atttacactt cccgtcttga
      241 tgatacaccg cgcctgtcgc gatttttgcg ggggattttg tgatggatct cgcaaaaaac
      301 cggcacagcc aggtcgaaac gcataattat gacacaaaaa aacctgaatg gatatccagt
      361 atgcgaatgc aataaggtcg attggaaaga caattctgga atgcaattcg cacttatcaa
      421 cacgggtgtg gtttcgtact cagcgtggta aactgacgcg tttatactgt gtagaaatca
      481 atcatcggga gaagtgctca tggccagcag aggcgtaaac aaagtaattc tggtcgggaa
      541 tctgggtcag gatccagaag tccgttacat gccgaacggc ggcgcagtgg ccaacattac
      601 cctggcgacc tccgaaagct ggcgtgacaa agcgaccggc gaacagaaag agaagaccga
      661 gtggcaccgc gtcgtgctgt tcggcaaact ggctgaagtg gcgggcgaat acctgcgcaa
      721 aggctctcag gtctatatcg aaggctccct gcagacccgt aaatggcagg atcagagcgg
      781 ccaggatcgc tacaccaccg agatcgtggt taacgtcggc ggcaccatgc agatgctggg
      841 cggccgtcag ggcggcggcg caccggccgg tcaatctgcc ggcggccagg gcggttgggg
      901 ccagcctcaa cagccacagg gcggcaacca gttcagcggc ggccagcagc agtctcgtcc
      961 ggcgcagaac agcgctccgg cgaccagcaa cgaaccgcca atggatttcg acgacgatat
     1021 tccgttctaa gcctggcgac agcgctaaaa aaaccccgct caggcggggt tttttgtggg
     1081 cgctcactgc cgattttctg cggcggtgtc gccaatcagt aaattgctac cgggttgatg
     1141 ttaacctggg ttgaaccgac atacagcggt tgccccagca cgaacaggaa ttc
//