From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Oryctolagus cuniculus muscle inhibitor-2 mRNA, complete cds.
Date: 28 Aug 92 10:01:07 GMT
Distribution: bionet
Checksum: 27510     3

LOCUS       RABMUSINHB    615 bp ss-mRNA            MAM       28-AUG-1992
DEFINITION  Oryctolagus cuniculus muscle inhibitor-2 mRNA, complete cds.
ACCESSION   M95581
KEYWORDS    inhibitor-2.
SOURCE      Oryctolagus cuniculus (library: lambda gt11) skeletal muscle cDNA
            to mRNA.
  ORGANISM  Oryctolagus cuniculus
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Lagomorpha; Leporidae.
REFERENCE   1  (sites)
  AUTHORS   Cohen,P.
  TITLE     The protein phosphatases involved in cellular regulation. Primary
            structure of inhibitor-2 from rabbit skeletal muscle
  JOURNAL   Eur. J. Biochem. 155, 173-182 (1986)
  STANDARD  full staff_review
REFERENCE   2  (sites)
  AUTHORS   Cohen,P.
  TITLE     Analysis of the in vivo phosphorylation state of protein
            phosphatase inhibitor-2 from rabbit skeletal muscle by fast atom
            bombardment mass spectrometry
  JOURNAL   Biochim. Biophys. Acta 929, 208-219 (1987)
  STANDARD  full staff_review
REFERENCE   3  (bases 1 to 615)
  AUTHORS   Lee,E.Y.
  TITLE     PCR cloning of the coding region of the cDNA for rabbit muscle
            inhibitor-2
  JOURNAL   Unpublished (1992)
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     CDS             1..615
                     /note="fast atom bombardment mass spectrometry indicates
                     that about 50% of the inhibitor-2 molecules have an
                     additional serine residue at the C-terminus (Biochimica et
                     Biophysica Acta 929: 208-219 (1987))"
                     /note="residues 1-25 and 586-615 are derived from the
                     primer sequences used"
                     /product="inhibitor-2"
                     /codon_start=1
                     /translation="MAASTASHRPIKGILKNKTSSTSSRVASAEQPRGSVDEELSKKS
                     QKWDEMNILATYHPADKDYGLMKIDEPSTPYHSMIGDDDDAYSDTETTEAMTPDTLAK
                     KLAAAEGSEPKYRIREQESSGEEDSDLSPEEREKKRQFEMKRKLHYNEGLNIKLARQL
                     ISKDLHDDEEDEEMSETADGESMNTEESNQGSTPSDQRQNKSQS"
                     /citation=[3]
BASE COUNT      218 a    137 c    152 g    108 t
ORIGIN
        1 atggccgcct ccaccgcctc ccaccggccc atcaagggga tcctgaagaa caagacctcc
       61 tcgacttcct ccagggtggc ctcggccgag cagccccgcg ggagtgtgga cgaggagctg
      121 agtaaaaaat cacagaagtg ggatgaaatg aacatcctgg caacctatca tccagcagac
      181 aaagactatg gtttaatgaa aatagatgaa ccaagcactc cttaccatag tatgataggt
      241 gatgatgatg atgcatacag cgatacagaa accactgaag ccatgactcc agacacctta
      301 gctaagaaat tggctgcggc tgaaggctca gagccaaagt atcggattcg ggaacaggaa
      361 agcagtggag aggaggatag tgacctctca cccgaagaac gagaaaaaaa gcgacaattt
      421 gaaatgaaaa ggaagcttca ctacaatgaa ggactaaata ttaaattagc tagacagtta
      481 atttcgaaag acctacatga tgatgaggaa gatgaagaaa tgtcagagac tgcagatgga
      541 gaaagcatga atacagaaga atcaaatcaa ggatctactc caagtgacca gcgccagaac
      601 aagtcccagt cctag
//