From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: E. coli primosomal replication protein n' (priC) gene.
Date: 5 Jan 93 06:46:23 GMT
Distribution: bionet
Checksum: 54513 4

LOCUS       ECOPRIC      1225 bp ds-DNA             BCT       05-JAN-1993
DEFINITION  E. coli primosomal replication protein n' (priC) gene.
ACCESSION   D13958 D00727
KEYWORDS    primosomal replication protein n'.
SOURCE      E. coli (strain K12; cell line HMS-83) genomic DNA, clone
            pBSndb-12.
  ORGANISM  Escherichia coli
            Prokaryotae; Bacteria; Gracilicutes; Scotobacteria; Facultatively
            anaerobic rods; Enterobacteriaceae.
REFERENCE   1  (bases 1 to 1225)
  AUTHORS   Zavitz,K.H., DiGate,R.J. and Marians,K.J.
  TITLE     The PriB and PriC replication proteins of E. coli: Genes, DNA
            sequence, overexpression, and purification
  JOURNAL   J. Biol. Chem. 266, 13988-13995 (1991)
  STANDARD  full automatic
COMMENT     Submitted (31-OCT-1990) to DDBJ by: Kenneth J. Marians Memorial
            Sloan-Kettering Cancer Center Rockefeller Research Laboratory 430
            East 67th Street New York, NY 10021 U.S.A. Phone:  212-639-5890
            Fax:    212-717-3627.
FEATURES             Location/Qualifiers
     -10_signal      392..397
                     /note="pot. -10 box"
     RBS             406..409
                     /note="pot. ribosome binding site"
     CDS             416..943
                     /gene="priC"
                     /product="primosomal replication protein n' (gtg start
                     codon)"
                     /codon_start=1
                     /translation="VKTALLLEKLEGQLATLRQRCAPVSQFATLSARFDRHLFQTRAT
                     TLQACLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
BASE COUNT      328 a    315 c    339 g    243 t
ORIGIN      
        1 gaattcgcca catatagaaa agcaaacagg cgagaattac cagcaacatg atgcgcaccc
       61 atggcatctg gacgaaccac agagaaatgg caaacgtgag caaaataagc aaaatcgccc
      121 gcggtttgac gccgcgccgc atcgcatgat gtttctgcca gaaacgtaga tagctgccaa
      181 accatgagcg gtacagcaac caggcgtgaa agcgcgggga agaacgggca aagcaccagg
      241 ccgccagcag gataaacggc gtcgtcggta ataccggtaa taccacgccc agcgtaccca
      301 gcactaccgc cagccagcca atgatgatta aaatgattcg ttgcatattg agtgttgtca
      361 gcttacagag tggctacttt agcataacaa ttatcatttt cattgaggtc ttatcgtgaa
      421 aaccgccctg ctgctggaaa aactggaagg acagctcgct acgctgcgtc agcgttgtgc
      481 cccggtgtca cagttcgcca cgctaagtgc tcgtttcgac aggcatcttt ttcagactcg
      541 tgcgacaaca ctacaggctt gtctcgacga ggcgggcgat aatctggctg cgcttcgtca
      601 tgcagttgag cagcaacagc tgccgcaagt ggcctggctg gcggaacatc tggcggcaca
      661 actggaagcc atcgcgcgtg aagcctccgc ctggtcattg cgcgagtggg acagtgcacc
      721 accgaaaatt gcccgctggc agcgtaaacg tattcagcat caggattttg agcggcggct
      781 acgtgagatg gttgccgaac gcagagcccg tctggcgcgg gtgaccgatc tcgtggaaca
      841 gcaaacgctg catcgtgaag tggaagccta tgaagcgcgc ctggcacgct gccgccatgc
      901 gctggaaaaa atcgaaaaca ggttagcgcg tttaacccgc tagcaatgga gagaatatgt
      961 cactggaaaa tgcccctgac gatgtcaaac tggctgtcga tttgattgtg ctgctggagg
     1021 aaaatcagat ccctgccagt accgtgctgc gcgcgctgga tattgtaaag cgtgattatg
     1081 aaaagaaatt aacgcgcgat gatgaggcgg aaaagtgaga aataaatggg gtcgctaaat
     1141 gtcccctaag gttgcgggca aacgcaaaac tgcctgatgc gctacgctta tcaggcctac
     1201 accgctcctg caatatattg aattc
//