From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Schizophyllum commune (strain UVM 9-1, clone cosmid W2R6)
Date: 15 Sep 92 10:06:06 GMT
Distribution: bionet
Checksum: 64390    13

LOCUS       SCOMEPAY1    5872 bp ds-DNA             PLN       15-SEP-1992
DEFINITION  Schizophyllum commune (strain UVM 9-1, clone cosmid W2R6)
            metalloendopeptidase (MEP) gene, 3'end; A-alpha Y1 protein
            (AalphaY1) gene, complete cds.
ACCESSION   M97179 M80789
KEYWORDS    A-alpha Y1 protein; A-alpha Y1 protein basic domain;
            A-alpha Y1 protein homeodomain region;
            A-alpha Y1 protein serine-rich domain; metalloendopeptidase.
SOURCE      Schizophyllum commune (strain UVM 9-1) homokaryon haploid DNA.
  ORGANISM  Schizophyllum commune
            Eukaryota; Fungi; Eumycota; Basidiomycotina; Hymenomycetes;
            Agaricales; Schizophyllaceae.
REFERENCE   1  (bases 1 to 5872)
  AUTHORS   Giasson,L., Specht,C.A., Milgrim,C., Novotny,C.P. and Ullrich,R.C.
  TITLE     Cloning and comparison of A-alpha mating-type alleles of the
            Basidiomycete Schizophylum commune
  JOURNAL   Mol. Gen. Genet. 218, 72-77 (1989)
  STANDARD  full automatic
REFERENCE   2  (bases 1 to 5872)
  AUTHORS   Specht,C.A., Stankis,M.M., Giasson,L., Novotny,C.P. and
            Ullrich,R.C.
  TITLE     Functional Analysis of the Homeodomain-related Proteins of the
            A-alpha Locus of Schizophyllum commune
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. (1992) In press
  STANDARD  full staff_review
REFERENCE   3  (bases 1 to 5872)
  AUTHORS   Stankis,M.M., Specht,C.A., Yang,H., Giasson,L., Ullrich,R.C. and
            Novotony,C.P.
  TITLE     The A-alpha mating locus of Schizophyllum commune encodes two
            dissimilar, multiallelic, homeodomain proteins
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 89, 7169-7173 (1992)
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     misc_feature    join(1876..1909,1960..2073,2124..2159)
                     /function="mating type"
                     /note="Homeodomain."
                     /product="A-alpha Y1 protein homeodomain region"
                     /note="putative"
                     /label=homeodomain
                     /citation=[3]
                     /citation=[2]
     CDS             join(1445..1909,1960..2073,2124..2183,2233..2787,
                     2841..2915,2964..3233,3283..4187,4239..4470)
                     /function="mating type"
                     /standard_name="A-alpha Y1"
                     /product="A-alpha Y1 protein"
                     /gene="AalphaY1"
                     /evidence=EXPERIMENTAL
                     /codon_start=1
                     /translation="MTDRLASLRAISASAKSMMSIAAARGAQPTAPIQTAPVHFDPLP
                     TPYLDGIRARLTEVKLPPKALKSALNSYDQACARWRYSLDESFSQAARSVSPHNLHLL
                     STLRFRLYTEQVERWAVQVLQVAEQWRAEMEKQRAHIAASTDKSKKPRPKFHSEYTPL
                     LELYFRFNAYPTYADRRVLAEKTGMLTRQITVWFQNHRRRAKGPLPRMTPTAKIPLEE
                     FERERENLARKLLPMLLPPHLRPITLGNNKTPDLTTSSRARALPPAKEDKPPQVTRKT
                     SKKVPKTAHPAPSTLVAPSQDTVMADAISTKKTKKAKSKQCADIEMKDSSKPKRRKMK
                     KLPKGVVGTADVAMCIDPPQVPKKNKVKPKKSMAFDSQAELAFAQAAYPSPSKYAWVH
                     TRKPQTDAPKAKASSVTSDVARLGKGRPGKPSPPASSTVPSRRVSTRLNAMRPPYAFP
                     ARYDSAAVPMTFAVAQVTKFTFATDSASFGFKPRISTRRPNITSDAMSQLVSSFERLR
                     LLSVELSVSRNPPLPAFEHQRLVDLRVEGLTSGEVTALHFTPGAYAARLAVTYIPPRA
                     PLPSTVLDLPRALRARLVHPMVLPETVTQPDAFAPFIALAERRARRKARKEKKRQEEK
                     QAKKDKKERKKAGLPHRAPSTVDAPDVSSRASSLDSDVSTSARKSSKKSKRQPSSSSR
                     ASSVASSGRTPSLSSTSSRRSSGMSMPSTPGPEQSLPIVAASDFALGGEEDVSMDADL
                     MAQLFGSDENADAVGYDLPMHPEPFTADMITFTSCADGALGDMTADVNMPNLGQSSID
                     DMNWTASVGSNAQDPASQESGGDEASHWLDISFDRPTTTSQINVLGGTYSCELGGSDN
                     MNAPLDFSDLTFGLDTGADYFSGFNNTIGGTTIMV"
                     /citation=[1]
                     /citation=[3]
                     /citation=[2]
     CDS             join(3..177,231..862)
                     /function="cleaves oligopeptides"
                     /standard_name="metalloendopeptidase"
                     /note="Identity to Rat testes MEP, Biochem. 29:
                     10323-10329 (1990)"
                     /product="metalloendopeptidase"
                     /gene="MEP"
                     /note="putative"
                     /codon_start=1
                     /translation="IPDQFEAVGRGPIPGLPGTYQQPLVVLLCEFARPSLGAAVLEWH
                     EVMTLFHEMGHAMHSMIGRTEYQNVSGTRCPTDFVELPSILMEHFLNSRQVLSLFHAD
                     STSSSSQPIGNQHEDPCHSIDTYAQIMLAALDQIYHSPAALEPGFDSTRELARLHDEK
                     GLIPYVPGTSFQTQFGHLFGYGATYYSYLFDRAIASRVWKDVFSSSPLSRETGERYKQ
                     EVLQYGGGKDPWEMVSALLKAPELASGDAEAMATVGRWKIEDEVGLPGRH"
                     /citation=[3]
                     /partial
     misc_feature    3565..3648
                     /function="mating type"
                     /note="Basic region."
                     /product="A-alpha Y1 protein basic domain"
                     /evidence=EXPERIMENTAL
                     /citation=[3]
                     /citation=[2]
     misc_feature    3697..3873
                     /function="Mating type"
                     /note="Serine-rich region."
                     /product="A-alpha Y1 protein serine-rich domain"
                     /evidence=EXPERIMENTAL
                     /citation=[3]
                     /citation=[2]
BASE COUNT     1219 a   1855 c   1525 g   1273 t
ORIGIN
        1 gaattccgga tcaattcgag gcggtcggtc ggggaccgat acctggactg ccggggactt
       61 accagcagcc cttggtcgtc ttgctctgcg agtttgcgcg cccttcgctc ggcgcggcgg
      121 tcctcgaatg gcacgaagtt atgaccctct tccatgagat gggccatgcc atgcattgta
      181 agcctacatt cctcactcct cctttctgtg cgctaacctc tcacctccag ccatgatcgg
      241 ccgcaccgag taccagaacg tctccggtac ccgttgccct actgacttcg tcgagctccc
      301 ctccatcctc atggagcact tcctcaactc ccgccaagtg ctctccctct tccacgccga
      361 ctccacctct tcttcctccc agcccatcgg caaccagcac gaggacccgt gccactccat
      421 cgacacgtac gcgcagatca tgctcgccgc cctcgaccag atctaccact cgcccgccgc
      481 gctcgagccc gggtttgact cgacgcgcga gctggcgagg ttgcacgatg agaaggggct
      541 gatcccgtac gtgcccggga cgagcttcca gacgcagttc gggcacttgt tcgggtatgg
      601 ggcgacatac tactcgtact tgttcgaccg cgcgatcgcg tcgagggtgt ggaaggacgt
      661 tttcagctcg tcgccgctca gtcgtgagac gggcgagcgg tacaagcagg aggtgctgca
      721 gtacggtggt ggcaaggacc catgggagat ggtcagcgcg ttgctgaagg cgccggagct
      781 ggcgagtggg gatgcagagg cgatggcgac tgtcgggcgg tggaagatcg aggatgaagt
      841 cggcttgcct ggcaggcact agatatacta ccatactgac gctcacatcg acacactact
      901 tactgtacgc ataatcctca atgcatctgt agcccactcg cgtcatgtcc gctgctatgg
      961 cgtgttgcca cggtcgcccg tgttcaagtg cctgatgtta ggaagacgtc gcatcggcag
     1021 agctttcgca cgtacgaccg aagggcatgc taagcagaca ggtatgacct gcgatgcagc
     1081 acaattggca acttagtaga tggcccgcgc gcaggccgta gaccattgac ccagcgtcac
     1141 acaacatggg ccggggcttg gaaggcccgc cacacatctc agtaatcagg ccatctcgaa
     1201 caagcggtgg aaggaagttg ttgttctaat tgtgatcggt tgctggccag ccttgtacgg
     1261 gatggaattt atgttgttct ccaaatggag taatcactgc gtgtgtggcg aggttgtgct
     1321 tatataagcg tggccaagct cgtggtgatc ggaagtaaac aagggtcaag tgatataacc
     1381 tgggcggcct cacggtctcc gttgcctact tccttccccc ctacagcccc tcgtcatgcc
     1441 cgtcatgacg gaccgactgg ctagccttcg ggccatatct gcctcagcga agagcatgat
     1501 gtctatcgct gccgcgcgtg gcgcgcagcc cactgctccc atccaaactg ctcccgtaca
     1561 cttcgaccct cttcccacac cctacctcga tggcatccgc gcccgcctca ccgaggtcaa
     1621 gctccctcca aaggcactca aatctgcttt gaactcgtac gaccaagcct gcgctcgatg
     1681 gcgatacagc ctcgacgagt cgttctcgca agccgcgcgc tccgtatctc cccacaacct
     1741 gcatcttctc tcgacactgc gcttccgtct atataccgag caggtcgaga gatgggcggt
     1801 ccaggtgctg caagttgcag agcaatggag ggccgagatg gagaagcagc gcgcgcacat
     1861 agccgcgtct accgataaat cgaagaagcc tcggcctaag ttccattccg tgagtgtgca
     1921 ctaccctgcc caaaggcttc gctgacactc ttcacacagg aatatacacc cttgctggag
     1981 ctgtacttcc gcttcaacgc ataccctacc tacgccgatc gccgcgtcct tgctgagaag
     2041 accggcatgc tcaccaggca aattactgtc tgggttagcc ttccgtcatt ccatcctcgt
     2101 cgcatgtaac tgacgatcta cagtttcaga accaccgccg ccgggcgaag ggaccgcttc
     2161 ctcgcatgac ccccacagca aaggtgcggt gacgacgaac gatcatggga gcatactaat
     2221 ctatcacttt agatcccgtt ggaggaattt gagcgtgaac gggagaactt ggcgcgcaag
     2281 ctgctgccca tgctccttcc cccgcacctc cgaccgatta cactgggcaa caacaagacc
     2341 cccgatctca ccaccagctc ccgcgctcgt gccttgccgc ccgccaaaga ggacaagcca
     2401 ccgcaggtca ctcgcaagac gtcgaagaag gtgccgaaga ccgcgcaccc ggccccgtct
     2461 acccttgtcg cgccgtcgca agatactgtc atggcggacg caatctcaac gaagaaaacg
     2521 aagaaggcga agtccaagca gtgcgctgat atcgagatga aggatagctc gaagcctaag
     2581 cgccgcaaga tgaaaaagct gcccaagggc gtcgtcggga ccgccgatgt cgcgatgtgc
     2641 atcgatcccc cgcaagtgcc gaagaagaac aaagtcaagc ccaagaagtc tatggcgttc
     2701 gattcgcaag cagagctcgc ctttgcccag gcagcgtatc cttcaccctc caagtacgcc
     2761 tgggtacata ctcgcaaacc tcagacggta agccttcgaa gcctcgctgg attgacaagc
     2821 gccctcatca cgatttccag gatgcaccca aagctaaagc aagctctgtc acatccgatg
     2881 ttgcgcgttt agggaagggg aggcccggaa agcctgtaag tctatctgca atcgctggat
     2941 tcggttgact cagctttgca cagagccctc cagcatcgtc caccgtcccc tctcgccgtg
     3001 tgtccacccg tctgaatgcc atgaggcctc cttatgcgtt ccctgcccgc tacgactctg
     3061 ccgctgtacc catgacgttc gctgtcgccc aggttacgaa gttcacattc gcaaccgact
     3121 cagcgtcgtt cggcttcaag ccccggattt cgaccagacg accaaacatc acctcggatg
     3181 ctatgtccca actcgtgtcg agtttcgaac gcctccgctt gttaagcgtc gaggtaagtg
     3241 ttgaatgctt atctgatata cctactctaa tccccctcac agctctccgt gtcccgcaat
     3301 cccccgctcc ccgccttcga acatcaacgt ctcgtcgacc tgcgtgtcga gggcctgacc
     3361 tctggagaag tcacggccct ccacttcact ccgggcgcgt atgctgcgcg tctggcggtg
     3421 acctatatcc cacctcgtgc ccccctccct tctactgtgc tggaccttcc gcgcgctctg
     3481 cgtgcgcgcc ttgttcaccc tatggtgctt cccgagaccg tcacgcagcc ggacgcattc
     3541 gctccgttca tcgctttggc ggaacgacga gcgaggcgca aggctcgcaa ggagaagaag
     3601 aggcaggagg agaagcaagc gaaaaaggac aagaaggagc gaaagaaggc gggtctaccc
     3661 caccgcgcgc catccaccgt agatgcgccg gacgtatcga gccgcgcatc ctcgctcgac
     3721 tccgatgtct cgacgagcgc tcgcaagtcc tcgaagaagt ccaagcgcca gccatcgtct
     3781 tcgtcacgcg catcctccgt tgcctcgtcg ggtcgcacac cgtccttgtc ctccacgtct
     3841 tcgcggcgct cttcgggcat gagcatgccg agtacccctg gcccggagca aagcctaccg
     3901 attgtagctg cctccgactt cgccctcggc ggcgaagagg acgtctccat ggatgcagac
     3961 ctcatggctc agctgttcgg cagcgatgag aatgcggacg ctgttggtta cgacctgccc
     4021 atgcatcccg agccatttac cgcagacatg atcacattca ccagctgcgc cgacggcgcc
     4081 ctcggcgaca tgacggcaga cgtcaatatg cccaacctgg gccagtcttc aatcgacgac
     4141 atgaattgga ctgccagcgt tgggtccaat gcccaggatc ctgcttcgtt tggctcgtcc
     4201 gaaaatacga cgaacatgga cctcaactgg cttctcagcc aggagagtgg cggcgacgag
     4261 gcctcccact ggctcgatat ctcatttgat cggcctacta ccacgtcgca gatcaatgtc
     4321 ctgggcggca cgtactcgtg tgagctcggc ggatccgaca acatgaatgc cccgctggac
     4381 ttcagcgact tgaccttcgg gctcgacaca ggcgctgact acttctctgg cttcaacaac
     4441 accatcggcg gcacgacaat catggtgtaa agcacgagca ccgccataca gtctccccca
     4501 tctacccttt ttgtcagcct cgcagcacat cactcacgta agtcatagcc aacgcgatgt
     4561 gattcctgaa ggagctgaca ctacatagga catcagcgcc aacgtaccgt cgttcgtggc
     4621 ggtatctcct tccccttccc cttttactag catcgctgca caacccctat gtaagtcccc
     4681 tttgtatacg atctgggtta cctaaaggct gacattgcgc aggctatcac cattgacacg
     4741 tcgttttcaa cgacggctca aatcatgcga catcttggcg cgaggccttc gcccccgtcc
     4801 tgcgataact cacctatgac atgcgtcttc tatgcgacca tgcgtatgct ctcttacctc
     4861 ttagtcttct tgcatcgttc tcagtctctt cgtcttgctc tgactgctgt gtcgctatcc
     4921 gttgctttcg gctatcttcg ttttcgttgc tttccactcc tggtgcattc acttcatctt
     4981 atttttatac atattttaat ccactactgc atcttctggt ctcgttgcac atatcggata
     5041 caatgtactt ttaggaactc aaccactgta ctataattca tcttcttggt caccgtcgcg
     5101 tcggatgtgg ttccggtcgt gggcaacgtc catattcatc catcgcaaac gacaacttca
     5161 tctggtgtgc cgtcgtgatc gtgcgacgca atcctcaggc attggcggta ccgttgctcc
     5221 tcaatgaccc aagatgcggg tctgcccttc ccaatctcgc gagcttgttc acccacgcag
     5281 ataacgagct cctctacggg gttcccttgc ttcagcatct tgagagtatg ccaaagcttc
     5341 ttggccagac gtgtattccg gccaataagc tctctcggtt cttccattcc atcctccagt
     5401 cgatcgaaat cgagcggaat gttgagggtg agcttgcgca gggcgtggaa ctgcgcgagc
     5461 gccaggcaaa ttccatcatc atcagtcggc cacccatcaa ggtcgaaggc aaagttggtt
     5521 agattaggac agcaagagtc gatggcaacg atctcgtcca tggtcaatgc atgtcgaagg
     5581 acgagaccag tgtacgaagg atattccact tcgtgaatgt gcagcgcccg tatctgatgt
     5641 ccctggtgtc gcaggacgcc ttccagcggt cgcaagtcat gtagatcaag cacagacagc
     5701 cactgaaggg aggggagagc gatgagaaaa gccctgacac tgtcgatggt gcgctcgcgc
     5761 tctgctgatg gcagatccgt ttccgtgagt cggtcgaagg ccagttggac ggtggagagt
     5821 gaggcgaaca tgctggcatc ccaaggaagg gtctggagat ccggaattga tc
//