From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Oryzias latipes hatching enzyme constituent protease (hce) mRNA,
Date: 3 Jan 93 22:18:18 GMT
Distribution: bionet
Checksum: 23230 4

LOCUS       ORZHCEB       940 bp ss-mRNA            VRT       23-DEC-1992
DEFINITION  Oryzias latipes hatching enzyme constituent protease (hce) mRNA,
            complete cds.
ACCESSION   M96171
KEYWORDS    hatching enzyme; protease; zinc metalloprotease.
SOURCE      Oryzias latipes pre-hatching embryo exocrine gland cDNA to mRNA.
  ORGANISM  Oryzias latipes
            Eukaryota; Animalia; Chorqata; Vertebrata; Osteichthyes;
            Actinopterygii; Atheriniformes; Cyprinodontoidei; Oryziatidae.
REFERENCE   1  (bases 1 to 940)
  AUTHORS   Yasumasu,S., Yamada,K., Akasaka,K., Mitsunaga,K., Iuchi,I.,
            Shimada,H. and Yamagami,K.
  TITLE     Isolation of cDNAs for LCE and HCE, two constituent proteases of
            the hatching enzyme of Oryzias Latipes, and concurrent expression
            of their mRNAs during development
  JOURNAL   Dev. Biol. 153, 250-258 (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     sig_peptide     26..85
                     /gene="hce"
                     /codon_start=1
     misc_feature    86..262
                     /gene="hce"
                     /note="propeptide"
     misc_feature    212..214
                     /gene="hce"
                     /note="glycosylation site"
     mat_peptide     263..862
                     /gene="hce"
                     /codon_start=1
     misc_binding    557..559
                     /bound_moiety="zinc"
                     /gene="hce"
     misc_feature    560..562
                     /gene="hce"
                     /note="active site"
     misc_binding    569..571
                     /bound_moiety="zinc"
                     /gene="hce"
     polyA_signal    906..911
                     /gene="hce"
     CDS             26..865
                     /gene="hce"
                     /product="protease"
                     /codon_start=1
                     /translation="MNLASSACLLLLFLLGIAQALPVQNEEGHEEGNKEGHGEEGVEE
                     GDEDDFVDFTTRILTSNNNTDQLLLEGDLVAPTNRNAMKCWYNSCFWKKASNGFVVIP
                     YVISSQYSRGEVATIEGAMRAFNGRTCIRFVRRTNEYDFISVVSKNGCYSELGRKGGQ
                     QELSLNRGGCMYSGIIQHELNHALGFQHEQTRSDRDSYVRINWQNIIPASAYNFNKHD
                     TNNLNTPYDYSSIMHYGRDAFSIAYGRDSITPIPNPNVPIGQRNGMSRWDITRSNVLY
                     NCR"
BASE COUNT      277 a    251 c    213 g    199 t
ORIGIN      
        1 tccgttctga ccctccacat ccaccatgaa cttggcttct tccgcctgcc tgcttttact
       61 gttcctgctc ggcatcgctc aggcgctgcc agtccaaaat gaagagggcc atgaagaagg
      121 caataaagaa ggtcatggag aagaaggcgt agaagaaggt gatgaagatg actttgtaga
      181 cttcactacc aggatcctca ccagcaacaa taacactgac cagctcttgc tagaaggaga
      241 cctggtggct cctacaaaca ggaatgccat gaagtgctgg tacaacagct gtttctggaa
      301 gaaagcctcc aacggttttg tggtgatccc ttacgtcatt agctctcagt actctagagg
      361 agaagtggcc accatcgagg gcgccatgag ggcttttaac ggcagaacat gcatccgctt
      421 tgtccgtcgc accaacgagt acgacttcat tagcgttgtg agcaaaaatg gatgctactc
      481 tgagctgggc aggaagggag gacaacagga gctgtctctc aacaggggag gctgcatgta
      541 cagtggcatc atccaacacg aactcaacca cgctctgggc ttccagcatg agcagaccag
      601 gagcgaccga gacagttacg tccggatcaa ctggcagaac atcatcccag cgagtgccta
      661 caattttaac aagcatgata ccaacaacct gaacaccccc tacgactact cctccatcat
      721 gcactatgga agagatgcct tctccattgc ctatgggcga gattccatca cccccatccc
      781 caatcccaat gtccccatcg gccagaggaa tggcatgtcc cgctgggaca tcaccagaag
      841 caacgtcctt tacaactgtc gttaaaaatg tttatgtcaa gatctataat gtctcaatta
      901 ctacaaataa agtaaatgca aagcaaaaaa aaaaaaaaaa
//