From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Pisum sativum ascorbate peroxidase (ApxI) gene, complete cds.
Date: 3 Jan 93 22:08:04 GMT
Distribution: bionet
Checksum: 62318 9

LOCUS       PEAAPXIA     3625 bp ds-DNA             PLN       21-DEC-1992
DEFINITION  Pisum sativum ascorbate peroxidase (ApxI) gene, complete cds.
ACCESSION   M93051
KEYWORDS    ascorbate peroxidase.
SOURCE      Pisum sativum (strain Alaska) (library: lambda EMBL3) etiolated
            seedling bud DNA.
  ORGANISM  Pisum sativum
            Eukaryota; Plantae; Embryobionta; Magnoliophyta; Magnoliopsida;
            Rosidae; Fabales; Fabaceae.
REFERENCE   1  (sites)
  AUTHORS   Mittler,R. and Zilinskas,B.A.
  TITLE     Molecular cloning and nucleotide sequence analysis of a cDNA
            encoding pea cytosolic ascorbate peroxidase
  JOURNAL   FEBS Lett. 289, 257-259 (1991)
  STANDARD  full automatic
REFERENCE   2  (bases 1 to 3625)
  AUTHORS   Mittler,R. and Zilinskas,B.A.
  TITLE     Molecular cloning and characterization of a gene encoding pea
            cytosolic ascorbate peroxidase
  JOURNAL   J. Biol. Chem. 267, 21802-21807 (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     mRNA            join((895.897)..974,1175..1304,1454..1628,1731..1796,
                     2237..2285,2373..2458,2725..2804,2896..2998,3090..3148,
                     3242..>3242)
                     /gene="ApxI"
                     /partial
     misc_signal     573..581
                     /gene="ApxI"
                     /note="antiperoxidative element"
     CAAT_signal     721..725
                     /gene="ApxI"
     GC_signal       745..750
                     /gene="ApxI"
     misc_signal     838..851
                     /gene="ApxI"
                     /note="heat shock element"
     exon            (895.897)..974
                     /number=1
     intron          975..1174
                     /number=1
                     /gene="ApxI"
     exon            1175..1304
                     /number=2
                     /gene="ApxI"
     intron          1305..1453
                     /number=2
                     /gene="ApxI"
     exon            1454..1628
                     /number=3
                     /gene="ApxI"
     intron          1629..1730
                     /number=3
                     /gene="ApxI"
     exon            1731..1796
                     /number=4
                     /gene="ApxI"
     intron          1797..2236
                     /number=4
                     /gene="ApxI"
     exon            2237..2285
                     /number=5
                     /gene="ApxI"
     intron          2286..2372
                     /number=5
                     /gene="ApxI"
     exon            2373..2458
                     /number=6
                     /gene="ApxI"
     intron          2459..2724
                     /number=6
                     /gene="ApxI"
     exon            2725..2804
                     /number=7
                     /gene="ApxI"
     intron          2805..2895
                     /number=7
                     /gene="ApxI"
     exon            2896..2998
                     /number=8
                     /gene="ApxI"
     intron          2999..3089
                     /number=8
                     /gene="ApxI"
     exon            3090..3148
                     /number=9
                     /gene="ApxI"
     intron          3149..3241
                     /number=9
                     /gene="ApxI"
     exon            3242..>3242
                     /number=10
                     /gene="ApxI"
     polyA_signal    3415..3420
     CDS             join(1186..1304,1454..1628,1731..1796,2237..2285,
                     2373..2458,2725..2804,2896..2998,3090..3148,3242..3257)
                     /function="hydrogen peroxide removal; oxidative stress"
                     /EC_number="1.11.1.11"
                     /gene="ApxI"
                     /product="ascorbate peroxidase"
                     /codon_start=1
                     /translation="MGKSYPTVSPDYQKAIEKAKRKLRGFIAEKKCAPLILRLAWHSA
                     GTFDSKTKTGGPFGTIKHQAELAHGANNGLDIAVRLLEPIKEQFPIVSYADFYQLAGV
                     VAVEITGGPEVPFHPGREDKPEPPPEGRLPDATKGSDHLRDVFGKAMGLSDQDIVALS
                     GGHTIGAAHKERSGFEGPWTSNPLIFDNSYFTELLTGEKDGLLQLPSDKALLTDSVFR
                     PLVEKYAADEDVFFADYAEAHLKLSELGFAEA"
BASE COUNT     1021 a    548 c    609 g   1447 t
ORIGIN      
        1 atgataatat aggtcaagcc ttagccaaat tcaaaacaaa ttataaaata tttttattta
       61 ttttaaaata tttaatatga ataaatattt ttatgtaatg aacataatta taatttacgt
      121 tatcactcat tatttaaaaa ataaaataat aatttataag gttctacgaa aattgaatac
      181 attgtactaa aacaaattga atagattatc tttaagaata atattttatc atttaaacat
      241 ctaaaaaaaa taaaaataaa gttggcattt atcaataatt aattaatact aatattaatt
      301 ttaaaataaa aaaactaaga ataatttatt atcaatataa ataattttac atcattattt
      361 aatcaaatta aaatatttcg tcacgtcata ttaatatttt aaaatcaata tgctagtata
      421 acttaataga atatatattc gtaattaatt tataatataa aaatatttta tattatcaca
      481 tttttcttat ctaaataaga ataaaaatat tgtatgcacg actttctttt ccgaaataat
      541 acaaatacaa tattactata gtcaacaata gggctctgtc ataactcatc acgcaacgaa
      601 acccgttttc acgcattcaa aaactcacga cctttcgaat ttatatttct taattattcc
      661 attattactc catttctctc acttctctca ttcgtcgttc acgaaaccta cctcacctac
      721 ccaataatcc acacactgtg aatttagtca tttacacgtg tagattcatc ttaacattca
      781 actctccgaa tcaacaataa ccgtacgatt ccaaccatcc aacgtctcac agtaaagcta
      841 ctagaacttt ctcctcttcc ttcctcttta tattacgtgt tcttcatttc taacacacgc
      901 tcttcactct tggcttgtgc tctcctcgtg tcactagggt ttaacttctt cgtttttgct
      961 tcttagattt cgaggttcga acgtttcgct tttgattcag cttttttctt cgtcgtaggg
     1021 attgttaata ctcgaattgc agttgattat tttctagaat catttttttt agattagatc
     1081 catgttttga tcttgttttt gcttcgattt attggtatac gcgatgatct gattctgttt
     1141 gtgatgtatg gatttttgtt tgattttgct gtagagtcgt ttgctatggg aaaatcatac
     1201 ccaactgtta gtcccgatta ccagaaggcc attgaaaagg ctaagaggaa gctcagaggt
     1261 tttatcgctg agaagaaatg cgctcctcta attctccgtt tggcgtaagt tttgttatct
     1321 gcaatttttt tatagaatcg ttgatttgtg agattttgat tttttgaatt gtctgtgttt
     1381 aagtttttga gtgatttact ttgttgatga tttgtttgtg atattttatt tgcgatggtt
     1441 ttgttttgtg tagatggcac tctgctggta cttttgattc caagacaaag actggtggtc
     1501 ctttcggaac aattaagcac caagccgagc ttgctcatgg tgctaacaac ggtcttgata
     1561 tcgcggttag gctgttggag cctattaagg agcaattccc tattgtgagc tatgctgatt
     1621 tctaccaggt tggtaatttt tgtgtgtgtt tagtttttag atttgaattt atgtggttgt
     1681 tcaatttttg tgatcatgtg gttgatggtt tattttaata cgtaacgcag ttggctggtg
     1741 ttgttgctgt tgagattacc ggtggacctg aagttccttt ccaccctggt agggaggtat
     1801 gtttgaccac aactatcgct tttgtcttca aatctaattt acatgattag taaatcaatt
     1861 attgggtatc acttttttcg gttatataat gattggattc atgttggtgg gtaccttttt
     1921 ttttaaagaa tagtgtatgt ttaattttta tatcatgttc ggacattagt ttgtaagctt
     1981 tgatatttgt cactttttgg tgccttctgg ttttcaagaa tttccattgg ttacataatt
     2041 gcggtcagaa tcacaacaat taatctaatg tgatggaata attggaaatg cttttcttac
     2101 atgttttact aaaatgtatg taaagtgtgg ttatattatt ttacacagtt gttgatgatg
     2161 tattctttat ctttttttac tcagtttttc agatagttga agctataaca gtccttttgt
     2221 tttgttttca tatcaggaca agcctgagcc accacctgag ggtcgcttgc ctgatgccac
     2281 taagggtcag tgatctgatt tgtgatgtga attaatctat atgattgatg tatttatttg
     2341 tctaaggatt tgattcttga ttattattgc aggttctgac catttgaggg atgtgtttgg
     2401 aaaggctatg gggcttagtg atcaggacat tgttgctcta tctggtggtc acaccattgt
     2461 atgtcataac tttaagctcg ctcctacttt tattgtagta ttaataaaac cattaatatt
     2521 gagatttgca taatcatctg cattatgatt gtgcaattca ggatgtggat gttaatatta
     2581 ctactactaa tggggctgga atatcaaaag cttgatactt ctaactgtat aattcagctg
     2641 acttttgtgt aaatgttata tatgataatg tatttttgat gctctgggct taactctttc
     2701 ggaatagctt aatgtaattc ttagggagct gcacacaagg agcgttctgg atttgaggga
     2761 ccatggactt ctaatcctct catttttgac aactcatatt tcacgtaagt cttctaaaac
     2821 attatctttc aaccatgcca ccttttatct attttataaa tctcttcatt gacaacatta
     2881 ttaataaatg tatagtgagt tgttgactgg tgagaaggat ggccttcttc agttgccaag
     2941 tgataaggct cttttgactg actctgtatt ccgccctctt gttgagaaat atgctgcggt
     3001 aagtatattt ctatatttct attctatgtg aaattataat gtctactgta aaggtaataa
     3061 aaataactgt catctgtcaa tgtttacagg atgaagatgt tttctttgct gattatgctg
     3121 aagcacatct taagctctct gagcttgggt aggtcatttt atcaatttaa ctttggagct
     3181 tatgaagaat atacttgttt atagagagtt gattcatttt tatgtatttt ttaccttgca
     3241 gatttgctga agcctaagtc acagttgttt ggtgtttaga gaggagcact gtcctgaatc
     3301 ttacataaat ttcatagacg ttgcttttat tttcaatgtg gattcatctt agttgggtag
     3361 cattttggat gtattttgga agtttgattg ttttctctat tgttgatcct tggttaaata
     3421 acattgttaa gtggtaatgc ccagctattg cattttcctg ataattactg gttttattct
     3481 tattttgcgg gtgaaatttc atctaaagtg agccatgtat ttagtttaga ttaaaatttt
     3541 aaacatcatc cgttgataaa cttcaaatgt atgatatttc atttcttatc tcccatgctt
     3601 cagaaatggt gaaaactcta ttatt
//