From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Brucella abortus heatshock protein 60 (hsp60) gene, complete cds.
Date: 27 Jan 93 05:46:55 GMT
Distribution: bionet
Checksum: 15670 5

LOCUS       BRUHSP60A    1800 bp ds-DNA             BCT       26-JAN-1993
DEFINITION  Brucella abortus heatshock protein 60 (hsp60) gene, complete cds.
ACCESSION   L09273
KEYWORDS    chaperonin; heat shock protein 60.
SOURCE      Brucella abortus (strain 2308) (library: Roop Sau3A/BamHI pUC9 in
            E.coli) DNA.
  ORGANISM  Brucella abortus
            Prokaryotae; Gracilicutes; Scotobacteria; Neisseriaceae.
REFERENCE   1  (bases 1 to 1800)
  AUTHORS   Roop,R.M.II., Price,M.L., Dunn,B.E., Boyle,S.M.,
            Srirananganathan,N. and Schurig,G.G.
  TITLE     Molecular cloning and nucleotide sequence analysis of the gene
            encoding the immunoreactive Brucella abortus Hsp60 protein, BA60K
  JOURNAL   Microb. Pathog. 12, 47-62 (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     RBS             49..53
                     /standard_name="Shine-Dalgarno sequence"
                     /note="putative"
     terminator      1736..1752
                     /standard_name="rho-independent transcription terminator"
                     /note="putative"
     CDS             60..1700
                     /function="chaperonin"
                     /label=BA60K
                     /note="60 kD protein reactive immunoblots with sera from a
                     variety of experimentally and naturally infected hosts,
                     including humans, cattle, goats, dogs and mice.; putative"
                     /product="heat shock protein"
                     /codon_start=1
                     /translation="MAAKDVKFGRTAREKMLRGVDILADAVKVTLGPKGRNVVIEKSF
                     GAPRITKDGVSVAKEVELEDKFENMGAQMLREVASKTNDTAGDGTTTATVLGQAIVQE
                     GAKAVAAGMNPMDLKRGIDLAVNEVVAELLKKAKKINTSEEVAQVGTISANGEAEIGK
                     MIAEAMQKVGNEGVITVEEAKTAETELEVVEGMQFDRGYLSPYFVTNPEKMVADLEDA
                     YILLHEKKLSNLQALLPVLEAVVQTSKPLLIIAEDVEGEALATLVVNKLRGGLKIAAV
                     KAPGFGDRRKAMLEDIAILTGGQVISEDLGIKLESVTLDMLGRAKKVSISKENTTIVD
                     GAGQKAEIDARVGQIKQQIEETTSDYDREKLQERLAKLAGGVAVIRVGGATEVEVKEK
                     KDRVDDALNATRAAVEEGIVAGGGTALLRASTKITAKGVNADQEAGINIVRRAIQAPA
                     RQITTNAGEEASVIVGKILENTSETFGYNTANGEYGDLISLGIVDPVKVVRTALQNAA
                     SVAGLLITTEAMIAELPKKDAAPAGMPGGMGGMGGMDF"
BASE COUNT      437 a    528 c    524 g    311 t
ORIGIN      
        1 gaattccatt cacagtacaa catttacaaa tctgaccggg atattcccag gagagtaaaa
       61 tggctgcaaa agacgtaaaa ttcggccgca ctgcgcgcga aaagatgctg cgcggcgtcg
      121 atatcctcgc tgacgctgtt aaggtcacgc tcggcccgaa gggccgcaat gtcgttatcg
      181 agaagtcctt cggcgctccg cgcatcacca aggacggcgt ttcggtcgcc aaggaagtcg
      241 aactggaaga caagtttgaa aacatgggcg cacagatgct gcgcgaagtg gcttccaaga
      301 ccaacgatac tgccggtgac ggcaccacga ccgcgaccgt tctcggtcag gccatcgttc
      361 aggaaggcgc caaggccgtt gccgctggca tgaacccgat ggacctgaag cgcggcatcg
      421 acctcgctgt caacgaagtt gtggctgagc tgctgaagaa ggccaaaaag atcaacactt
      481 cggaagaagt tgcccaggtt ggcaccatct ctgccaacgg cgaagccgaa atcggcaaga
      541 tgatcgccga agcgatgcag aaggtcggca acgaaggcgt catcacggtt gaagaagcca
      601 agaccgccga aaccgaactc gaagtcgtcg aaggcatgca gttcgaccgc ggctacctgt
      661 cgccttactt cgtcaccaac cctgaaaaga tggttgctga cctcgaagac gcctacatcc
      721 ttctgcacga aaagaagctc tcgaaccttc aggctctcct gccggttctc gaagctgtcg
      781 tccagacctc caagccgctt ctcatcattg ctgaagacgt cgaaggcgaa gctcttgcaa
      841 cgctcgtcgt caacaagctg cgcggcggcc tgaagattgc tgccgtcaag gctccgggct
      901 tcggcgatcg ccgcaaggcc atgctcgaag acatcgcgat cctcactggc ggtcaggtca
      961 tctccgaaga cctcggcatc aagcttgaaa gcgttacgct cgacatgctg ggccgcgcca
     1021 agaaggtttc gatctccaag gaaaacacga cgatcgttga cggtgcaggc cagaaggccg
     1081 aaatcgacgc tcgcgttggc cagatcaagc agcagatcga agaaaccact tcggactacg
     1141 accgtgaaaa gcttcaggaa cgtcttgcca agctcgctgg cggcgttgcc gtgatccgcg
     1201 tcggcggtgc aacggaagtt gaagtgaagg aaaagaagga ccgcgttgac gacgccctga
     1261 acgcaacccg cgctgcggtt gaagaaggta tcgttgccgg cggcggcacc gccctgctcc
     1321 gcgcttcgac caagatcacc gcaaagggtg tgaatgccga ccaggaagct ggcatcaaca
     1381 tcgttcgtcg cgcgatccag gctccggccc gccagatcac gaccaatgcc ggtgaagaag
     1441 cttcggtaat cgttggcaag atcctcgaaa acacgtccga aaccttcggc tacaacacgg
     1501 ccaatggcga atatggcgac ctgatctcgc tcggcattgt tgacccggtc aaggttgtcc
     1561 gcacggctct gcagaacgca gcttcggttg ccggcctgct gatcacgacg gaagcaatga
     1621 tcgccgaact gccgaagaag gacgcagctc cggctggcat gcctggcggt atgggtggca
     1681 tgggcggcat ggacttctaa gaagtccata aagctcgccc atgaactaaa tgcctgcgcc
     1741 gcaaagcggc gcgggggcgg catggacttc caagaagtcc atgccaccgc aggacaggcg
//