From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: E.coli ethidium efflux gene for ethidium resistance protein
Date: 10 Apr 92 10:04:37 GMT
Distribution: bionet
Checksum: 05464     4

LOCUS       ECOETHRES    1244 bp ds-DNA             BCT       10-APR-1992
DEFINITION  E.coli ethidium efflux gene for ethidium resistance protein
ACCESSION   Z11877
KEYWORDS    ethidium efflux gene; ethidium resistance protein.
SOURCE      Escherichia coli DNA.
  ORGANISM  Escherichia coli
            Prokaryota; Bacteria; Gram-negative facultatively anaerobic rods;
            Enterobacteriaceae.
REFERENCE   1  (bases 1 to 1244)
  AUTHORS   Purewal,A.S.
  TITLE     Nucleotide sequence of the Ethidium efflux gene from Escherichia
            coli
  JOURNAL   FEMS Microbiol. Lett. 82, 229-232 (1991)
  STANDARD  full staff_review
REFERENCE   2  (bases 1 to 1244)
  AUTHORS   Purewal,A.S.
  JOURNAL   Unpublished (1992)
  STANDARD  full staff_review
COMMENT     *source: clone=pASP505; *source: is_macronuclear=N; *source:
            is_proviral=N; *source: is_germline=N;
            From EMBL    entry ECETHRES;  dated 29-MAR-1992.
FEATURES             Location/Qualifiers
     -35_signal      368..373
                     /citation=[1]
                     /citation=[2]
     -10_signal      389..394
                     /citation=[1]
                     /citation=[2]
     RBS             471..476
                     /citation=[1]
                     /citation=[2]
     CDS             486..818
                     /function="ethidium efflux"
                     /product="ethidiumresistance"
                     /gene="EB"
                     /evidence=EXPERIMENTAL
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLIIN
                     LLSRSTPH"
                     /citation=[1]
                     /citation=[2]
BASE COUNT      367 a    205 c    286 g    386 t
ORIGIN
        1 aagcttcttt cattcgctga aagtggaatg tatccatgga gaacacttta tcagccggga
       61 aataatgcgg gcaacggtgt ttaattatat cgaatgtgat tacaatcggt ggcggcggca
      121 cagttggtgt ggcggcctca gtccggaaca atttgaaaac aagaacctcg cttaggcctg
      181 tgtccatatt acgtgggtag gatcaaccag cataaatcag gctgcggcta aaatggcgcg
      241 ggcaggaatc ctggtcgttg atggtaaggt ctggcgaacg gtgtattacc ggttcgctac
      301 cagagaagaa tgggaaggaa aggtgagcac gaatctgatt tttaaggagt gtcgccagag
      361 tgccgcgatg aaacgggtat tgagggtata taaaagaaca tcaatgggaa cacaatgatg
      421 aaacaggtga gttgagttca aactgtagta caattctctc cagtttgaac aggaaagaat
      481 atgctatgaa cccttatatt tatcttggtg gtgcaatact tgcagaggtc attggtacaa
      541 ccttaatgaa gttttcagaa ggttttacac ggttatggcc atctgttggt acaattattt
      601 gttattgtgc atcattctgg ttattagctc agacgctggc ttatattcct acagggattg
      661 cttatgctat ctggtcagga gtcggtattg tcctgattag cttactgtca tggggatttt
      721 tcggccaacg gctggacctg ccagccatta taggcatgat gttgatttgt gccggtgtgt
      781 tgattattaa tttattgtca cgaagcacac cacattaaaa taatttgttt ctaaacgact
      841 aaaatatgga ggctcttata tttatatgag cctcgtttta tgctttttgt taatgtcttt
      901 attttttatg tattcttttg tgctttcaag attatggcgt aagaaaattg caatacatta
      961 ttgttgtata ttcaagataa tgtgacctta attgtctttt taaataaaaa ataaacaaaa
     1021 attatatccc accactaagg tttataaaag catacgttag caggtgtcac catgaaaaaa
     1081 gccatagcat atatgcgatt ttcatcacca ggtcagatgt ctggcgactc attaaaccga
     1141 cagagaagac ttattgctga atggttaaag gtaaatagtg attattatct tgataccata
     1201 acatatgaag atttaggatt aagtgcattc aaaggaaagc atgc
//