From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: P.mirabilis ssb gene for single-stranded DNA-binding protein (SSB)
Date: 10 Apr 92 10:11:09 GMT
Distribution: bionet
Checksum: 62386     4

LOCUS       PRMSSB       1511 bp ds-DNA             BCT       10-APR-1992
DEFINITION  P.mirabilis ssb gene for single-stranded DNA-binding protein (SSB)
ACCESSION   X65079
KEYWORDS    single-stranded DNA-binding protein; ssb gene.
SOURCE      Proteus mirabilis DNA.
  ORGANISM  Proteus mirabilis
            Prokaryota; Bacteria; Gram-negative facultatively anaerobic rods;
            Enterobacteriaceae.
REFERENCE   1  (bases 1 to 1511)
  AUTHORS   de Vries,J. and Wackernagel,W.
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
COMMENT     *source: strain=PG1300; *source: cell_line=E.coli DH1; *source:
            clone_library=plasmid gene bank;
            From EMBL    entry PMSSB;  dated 26-MAR-1992.
FEATURES             Location/Qualifiers
     CDS             complement(<1..354)
                     /gene="nurA"
                     /codon_start=1
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVITGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEP"
     misc_feature    complement(421..437)
                     /note="SOS-box(LEXA binding site)"
     promoter        439..467
                     /note="ssbPI"
     -10_signal      554..559
                     /note="ssbPN2"
     -10_signal      568..573
                     /note="ssbPN1"
     CDS             607..1131
                     /product="single-stranded DNA-bindingprotein"
                     /gene="ssb"
                     /translation="MASRGVNKVILIGNLGQDPEIRYMPSGGAVANLTLATSESWRDK
                     QTGEMKEKTEWHRVVIFGKLAEIAGEYLRKGSQVYIEGQLQTRKWQDQSGQDRYSTEV
                     VVNIGGTMQMLGGRGGQDNAPSQGQGGWGQPQQPQASQQFSGGAPSRPAQQPAAAPAP
                     SNEPPMDFDDDIPF"
     terminator      1168..1228
BASE COUNT      477 a    307 c    317 g    410 t
ORIGIN
        1 cggttcaccc acacgtgcaa ataatagacg taggtaatcg tggatctcgg taatggtacc
       61 gaccgttgag cgtgggttat gtgatgtcga tttctgctca atcgaaatag cgggcgataa
      121 cccttcaata tgatcaacat caggcttttc cattaaggaa agaaactgac gagcataagc
      181 tgaaagcgat tcaacgtaac gccgttggcc ttccgcataa agcgtatcaa aggctaaaga
      241 ggatttacct gagccagaaa gtcccgtaat aacaataagt ttatcgcggg gaatgaccaa
      301 attgatattt tttaggttat gggtgcgagc gccccgtact tcgattttat ccatgaatat
      361 atcccggatt aagtgagtcg ccgaaaaaga aagaatcgga taattatgac acaaaactag
      421 ctgaatggat atacagtata tatattgaaa ctgaatgata atgaaaattt gccatattaa
      481 gacaataaaa tgtcgatcct gttctcaatg gagaaattca ttactcatca tattcaaatt
      541 ttagcttacg tgataaactt catcgcttat gatttcaaaa aatttacttt cgcaggagta
      601 cctcacatgg cgagcagagg cgttaacaaa gttattctta tcggtaattt agggcaggat
      661 ccagaaatcc gttatatgcc aagtggtggt gcagtggcaa atctgacact agcaacttca
      721 gaaagctggc gcgacaaaca aaccggtgaa atgaaagaga aaaccgaatg gcaccgtgtg
      781 gtcattttcg gcaaactagc agaaattgct ggcgagtatc tgcgtaaagg ttcacaagtt
      841 tatattgaag gtcaattaca aacacgtaaa tggcaagatc aaagtggtca agatcgctac
      901 agcactgaag ttgtggtgaa tatcggtggc acaatgcaga tgttaggtgg ccgtggtggt
      961 caagataatg caccttcaca aggccaaggc ggttggggtc aacctcagca accacaagca
     1021 tcacaacagt ttagtggtgg cgcaccatct cgcccagcac aacaacccgc agcggcacca
     1081 gcgccaagta atgaaccacc aatggacttt gatgacgata ttccgttctg acggttactt
     1141 aagaacttac cttatcttct aagtcatata acatcaaacg ctaaagcctg ttatatcatt
     1201 aacgggcttt tctttgtttc atgcttatcc cgcatttgcc aatcatattg tcacaccttg
     1261 tctatttccc attgactatt actcgttcat cattggcgtt aagataagtc ttgtgaatga
     1321 gtattagccc ttgcaccatg gttagatttg atttactgat aatgacaatt tatagcgtta
     1381 ttttctcaaa atcccacttt ccacaaaggt ttgctcaaaa aaaatatggt aagatcatcg
     1441 taattaatca cgtctgagag catcctatcc tatgaatgaa gagaaattgc tcgctcaggc
     1501 agaaaagatc t
//