From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: E. coli acetylase (rimJ) gene, complete cds.
Date: 29 Dec 92 01:27:28 GMT
Distribution: bionet
Checksum: 53748 4

LOCUS       ECORIMJA     1609 bp ds-DNA             BCT       08-DEC-1992
DEFINITION  E. coli acetylase (rimJ) gene, complete cds.
ACCESSION   M99278
KEYWORDS    30S ribosomal subunit; N-terminal acetylation; acetylase;
            pap operon; ribosomal modification; ribosomal protein S18;
            rimJ gene; thermoregulatory protein.
SOURCE      Escherichia coli (individual_isolate MC4100, strain K-12) DNA.
  ORGANISM  Escherichia coli
            Prokaryotae; Gram-negative facultatively anaerobic rods;
            Enterobacteriaceae.
REFERENCE   1  (bases 1 to 1609)
  AUTHORS   White-Ziegler,C.A. and Low,D.A.
  TITLE     Thermoregulation of the pap operon: Evidence for the involvement of
            RimJ, the N-terminal acetylase of ribosomal protein S5
  JOURNAL   J. Bacteriol. 174, 7003-7012 (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     CDS             289..873
                     /gene="rimJ"
                     /product="acetylase"
                     /codon_start=1
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     CDS             884..1414
                     /standard_name="g20.3"
                     /codon_start=1
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRVPRRQVNCAAAPRECMNSAIWRKWSRRWNNWQIAKMVLLWCVWPANRVNAK
                     TATCIFSVVRLKISRR"
BASE COUNT      416 a    356 c    431 g    406 t
ORIGIN      
        1 gtcgatgagc aggaaatatt tacccaggtt cctcgcctgc gacacgcggg acatttccct
       61 cccgggaaat aaaagatgag cactttctat tctgcattgc cgctaagact tttcccatca
      121 cttcggggac aatatttttt tatcaaaacg tcgctttgat agagagtttt tatcaaatat
      181 gtgaataatg cagaaaatgg cattttggac ttttcacagg gtctggttgc gcaggtatag
      241 taatacttac agcgtattaa agacgttacg ggaaggagta ggtatagaat gtttggctat
      301 cgcagtaacg tgccaaaagt gcgcttaacc acagaccgac tggtcgtgcg tctggtgcat
      361 gatcgtgatg cctggcgtct tgcggattat tacgcagaga atcgccattt cctcaagccc
      421 tgggagccag tgcgcgacga aagccactgt tatccatcag gctggcaggc caggctgggg
      481 atgattaacg aatttcataa acaaggttca gctttctact ttggcttatt cgacccggac
      541 gaaaaagaga ttattggcgt tgccaatttt tccaatgttg ttcgtggctc ttttcatgcc
      601 tgctatctcg gttattcgat tgggcaaaaa tggcagggca aaggactcat gtttgaagcc
      661 ctgaccgcag ccattcgtta tatgcagcgc acccaacata ttcatcgcat tatggctaat
      721 tatatgccgc acaataaacg cagcggtgat ttactggcgc gactgggttt tgaaaaagaa
      781 ggctatgcga aagactatct gttgattgat ggacaatggc gcgatcacgt actgacggca
      841 ttaactaccc cagactggac gcccggccgc taaggagaat ctcatgaaat atcaacttac
      901 tgcccttgaa gctcgcgtga ttggttgctt gctggaaaag caggtgacca cgccggagca
      961 atacccgctc tcagtcaatg gtgtagtcac ggcctgtaat cagaaaacga accgtgaacc
     1021 ggtcatgaat ctgagtgaat ccgaagtgca ggaacagctg gataatctgg tcaaacgtca
     1081 ttatctacgc acagtgagcg gttttggtaa tcgggtcacc aaatatgagc aacgtttttg
     1141 taattcagaa tttggcgatc tgaaactgag cgcagcggaa gtggcgttaa tcaccacgtt
     1201 gttattgcgt gtgcccagac gccaggtgaa ctgcgcagcc gcgccgcgcg aatgtatgaa
     1261 ttcagcgata tggcggaagt ggagtcgacg ctggaacaac tggcaaatcg cgaagatggt
     1321 ccttttgtgg tgcgtctggc ccgcgaaccg ggtaaacgcg aaaaccgcta catgcatctt
     1381 ttcagtggtg aggttgaaga tcagccggcg gtgacggata tgtcgaacgc ggttgacggt
     1441 gatttacagg cccgcgtcga aggcctggaa atcgaagtgg cagaactgaa acagcgtctt
     1501 gattcgttgc tggccatctg ggagattaaa gtgaaaaaat tacgtatcgg cgtagtggat
     1561 taggtgcatt cgcaaaagcg tgttacgtct gcgcagctct gactgacgt
//