From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: A.fermentans GCDA gene for carboxytransferase subunit
Date: 3 Jan 93 22:03:23 GMT
Distribution: bionet
Checksum: 11818 5

LOCUS       AMCGCDA      2069 bp ds-DNA             BCT       21-DEC-1992
DEFINITION  A.fermentans GCDA gene for carboxytransferase subunit
ACCESSION   X69435
KEYWORDS    GCDA gene; UDP-galacturonate decarboxylase;
            biotin-containing carboxylase; carboxytransferase subunit.
SOURCE      Acidaminococcus fermentans DNA.
  ORGANISM  Acidaminococcus fermentans
            Prokaryotae; Gracilicutes; Scotobacteria; Anaerobic gram-negative
            cocci; Veillonellaceae.
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Bendrat,K. and Buckel,W.
  TITLE     Cloning, sequencing and expression of the gene encoding the
            carboxytransferase subunit of the biotin-dependent NA+PUMP
            glutaconyl-CoA decarboxylase from Acidaminococcus fermentans in
            Escherichia coli
  JOURNAL   Eur. J. Biochem. (1992) In press
  STANDARD  full automatic
COMMENT     *source: strain=ATCC 85025; *source: clone_library=EMBL12, Genbank;
            *source: sub_clone=pUC19;
               From EMBL    entry AFGCDA;  dated 30-NOV-1992.
FEATURES             Location/Qualifiers
     RBS             28..33
                     /note="putative"
     CDS             41..1804
                     /EC_number="4.1.1.67"
                     /gene="GCDA"
                     /note="alpha (carboxytransferase) subunit"
                     /product="UDP-galacturonate decarboxylase"
                     /codon_start=1
                     /translation="MGFYSMPRYFQNMPQVGKPLKKADAANEEQLKKIEEEIHQLIKE
                     AQEAGKADADVNKRGELTALQRIEKLVEPGSWRPLNTLFNPQGNKNGSVAIVKGLGRV
                     NGKWCVVVASDNKKLAGAWVPGQAECLLRASDTAKTLHVPLVYVLNCSGVKFDEQEKV
                     YPNRRGGGTPFFRNAELNQLGIPVIVGIYGTNPAGGGYHSISPTVIIAHEKANMAVGG
                     AGIMGGMNPKGHVDLEYANEIADMVDRTGKTEPPGAVDIHYTETGFMREVYASEEGVL
                     EGIKKYVGMLPKYDPEFFRVDDPKAPAFPADDLYSMVPLNDKRAYDIYNVIARLFDNS
                     ELHEYKKGYGPEMVTGLAKVNGLLVGVVANVQGLLMNYPEYKAAGSVGIGGKLYRQGL
                     VKMNEFVTLCARDRLPIVWIQDTTGIDVGNDAEKAELLGLGQSLIYSIQTSHIPQFEI
                     TLRKGTAAAHYVLGGPQGNDTNAFSIGTAATEIAVMNGETAATAMYSRRLAKDRKAGK
                     DLQPTIDKMNNLIQAFYTKSRPKVCAELGLVDEIVDMNKIRGYVEAFTEAAYQNPESI
                     CPFHQMILPRAIREFETFVKK"
BASE COUNT      541 a    560 c    562 g    406 t
ORIGIN      
        1 ccacggaagc aaaataagac attcgggagg aagaatttaa atgggtttct actctatgcc
       61 tagatatttc caaaatatgc ctcaggtggg caaacccctg aaaaaggcgg atgccgccaa
      121 tgaagaacag ctgaaaaaga tcgaagaaga gatccatcag ctgatcaaag aggctcagga
      181 agccggcaag gcagatgctg atgtaaacaa gagaggggaa ctgaccgctc tccagagaat
      241 tgaaaaactg gtggaaccgg gctcctggag acccctgaac accctgttca accctcaggg
      301 caacaagaac ggctccgtgg ccatcgtcaa gggcctgggc cgtgtaaacg gcaaatggtg
      361 cgtggtcgtg gcttccgaca acaagaagct ggccggtgcc tgggtgccag gccaggctga
      421 atgcctgctg cgggcttccg ataccgccaa gaccctgcac gtgcctctgg tgtatgtgct
      481 gaactgctcc ggcgttaagt tcgacgaaca ggaaaaggta taccccaacc gtcgtggcgg
      541 cggtacgcct ttcttccgga acgctgaact gaaccagctg ggcattcccg tcatcgttgg
      601 catctacggc accaaccctg ccggcggcgg ctatcattcc atcagcccca cggtgatcat
      661 tgcccatgaa aaagccaaca tggctgtggg cggcgccggc attatgggcg gcatgaaccc
      721 caaaggtcat gtggacctgg aatatgccaa cgaaatcgcc gacatggtgg accgtaccgg
      781 caagaccgaa cctccgggag ctgtcgacat ccactacacc gaaaccggtt tcatgcgtga
      841 agtctatgct tccgaagaag gcgttctgga agggatcaag aagtatgtag gcatgctgcc
      901 gaaatacgat cctgaattct tccgggtgga cgatccgaaa gcaccggcat tcccggctga
      961 cgacctgtac agcatggttc ctctgaacga caaacgggct tatgacatct acaatgtaat
     1021 tgcccgcctg ttcgacaaca gcgaactcca tgaatacaaa aaaggctacg gccctgaaat
     1081 ggtcaccggt ctggccaaag tcaacggcct gctggtgggc gttgtggcca acgtccaggg
     1141 tctcctgatg aactaccctg aatacaaggc tgcaggctcc gtagggatcg gcggcaaact
     1201 gtaccgccag ggtctggtga aaatgaatga attcgtcacc ctgtgcgcca gagaccgtct
     1261 gcccatcgtc tggatccagg ataccaccgg gatcgatgtg ggcaacgatg cggaaaaggc
     1321 tgaactgctg ggtctgggcc agtccctgat ctactccatc cagacttccc acatccctca
     1381 attcgaaatt accctgagaa aaggcacggc tgctgcccac tacgtactgg gcgggcctca
     1441 gggcaatgac accaacgcct tctccatcgg taccgctgct acggaaatcg ccgttatgaa
     1501 cggtgaaacc gctgccaccg ccatgtactc ccggagactg gccaaagacc ggaaagccgg
     1561 caaggatctg cagccgacca tcgacaagat gaacaacctg atccaggctt tctacaccaa
     1621 atctcgtccg aaagtctgtg ctgaactggg tctggtggat gaaatcgtgg acatgaacaa
     1681 gatccgcggg tatgtggaag ccttcacgga agctgcttat cagaatccgg aaagcatctg
     1741 cccgttccat cagatgattc tgcctcgtgc catcagagag tttgagactt tcgtaaagaa
     1801 ataatcctta cggaggaaat gttttaatga gtatctatac cttgggaatc gatgttggat
     1861 ctactgcatc caagtgcatt atcctgaaag atggaaaaga aatcgtggcg aaatccctgg
     1921 tagccgtggg gaccggaact tccggtcccg cacggtctat ttcggaagtc ctggaaaatg
     1981 cccacatgaa aaaagaagac atggccttta ccctggctac cggctacgga cgcaattcgc
     2041 tggaaggcat tgccgacaag cagatgagc
//