From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Plasmid pTiS4 (from Agrobacterium tumefaciens) T2 tryptophan
Date: 22 Dec 92 22:45:44 GMT
Distribution: bionet
Checksum: 07943 11

LOCUS       IS4AAHM      5516 bp ds-DNA             BCT       23-NOV-1992
DEFINITION  Plasmid pTiS4 (from Agrobacterium tumefaciens) T2 tryptophan
            monooxygenase (iaaM) and indole acetamide hydrolase (iaaH) genes,
            complete cds.
ACCESSION   M91609
KEYWORDS    iaaH gene; iaaM gene; indole-3-acetamide hydrolase;
            tryptophan monooxygenase.
SOURCE      Plasmid pTiS4 DNA.
  ORGANISM  Plasmid pTiS4
            Prokaryotae.
REFERENCE   1  (bases 1 to 5516)
  AUTHORS   Canaday,J., Gerard,J.-C., Crouzet,P. and Otten,L.
  TITLE     Organization and functional analysis of three T-DNAs from the
            vitopine Ti Plasmid pTiS4
  JOURNAL   Mol. Gen. Genet. (1992) In press
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     misc_signal     357..364
                     /note="Chi sequence"
     polyA_signal    complement(676..680)
                     /gene="iaaH"
     TATA_signal     complement(2276..2281)
                     /gene="iaaH"
     TATA_signal     complement(3182..3188)
                     /gene="iaaM"
     polyA_signal    complement(4817..4822)
                     /gene="iaaM"
     repeat_region   complement(5206..5277)
     CDS             complement(854..2242)
                     /function="auxin synthesis; root formation on host plants"
                     /gene="iaaH"
                     /product="indole acetamide hydrolase"
                     /codon_start=1
                     /translation="MVEISSISQTLRALRRKQYSCRDLVESLVSRSESATHLNAFAAT
                     DWLHLRNEADRVDRNGSGGVGLMGIPLCFKANIATGIFPTSAGTQGLLRHKPAIPAKI
                     VERLHSAGALIGASGNMHELSFGITNDNKTFGPARNPWNQALISGGSSGGVAVSVAAN
                     LMLGGIGTDTGASVRLPAALCGVVGFRPTFGNYPTDGIVPVSPSRDTPGLIVRSVEDA
                     VLLDRIIRNKCPTQNMSLKGLRLGLPRSHFFDNLEPHVAAASERAIRRLATNQMTFVE
                     ADIPNVAELTRKVSLPVAIYEFPRALMAYLSFHGIGNTFDELIQNIQDPQVYDLVQSQ
                     LSKGLISESVYRRALRCYKPRLKATYENYYSSNGLDAVLFPSVPLTAKPVGLQTTLVH
                     NGVETDTFGIFVRNLDPSSNIGLPSLSVPVSLTPDRLPVGIQIEGPFGSDDMVLAIGR
                     AVEEMVKFGQEY"
     CDS             2573..4744
                     /gene="iaaM"
                     /product="tryptophan monooxygenase"
                     /codon_start=1
                     /translation="MANFFYSRITNRSYSTKNLLNIEDKGRLKDELEKTRQTNICEIC
                     LHPRGHRASVCRQILMGFAYTSKTVLEGLLSTMPHDDAPLGKIFVTDLPPYDEQVPQV
                     LLMQAAALVTSYEYSFEDLAYFLVLPLQMALMQKSPSLGKDFPVISGYSITKDSVHSP
                     VAFGRNLMPRGVSCEFPQIDVLYDYRGFLEGGAFSEGVTSFPKETKKPKVAVIGAGIS
                     GLVSATLLLRNGIDDVTIFEAKNVVGGRAHTHFFKGEPSVCAELGAMRFPRSQACLFY
                     LLEYLGINAMTKFPNPGTVDTGLYYRGRSYNWKAHSLPPAIFNRVHKGWRTFLHAGFV
                     DGVAAFASPFTLTECLRLRNYEFASSLWQKWLDAFSSETFSSGIERIFRGAHPPGGEK
                     WTRDVDMELFKELGVGSGGFGPVFGCGFIEILRLIVNGYEDNVMLLLDGIEEIPRRLS
                     QQKVGSYSIRDRIIHKEVKEIIRTESGISLAIGEGMHATFDRVIVTSGFTNIQLRHLL
                     TNDDSFFSYDVNQAIENSHMTGSSKLFVLTQNKFWKAEELPSCILTTGVAKAVYCLDY
                     EPDKPSGKGLVLLSYTWEDDSHKLLTFDKGERFQILKRDLAKSYPRFADLLEPADGDY
                     DNNIIQHDWILDPYAGGAFKLNRRCEDVYSKRLFFQPLRLNGEPDGRVCLAGCSCSFS
                     GGWVEGAIQTACNAAMATIRDAGGLISGDNPLTNEFVNYHY"
BASE COUNT     1509 a   1266 c   1273 g   1468 t
ORIGIN      10.2 kb left of T-DNA T2
        1 gaattcttct tcgtgttcgt catagggcga aatcctatca aaggttaggg attcgctctc
       61 cacttcttcc gggcaagtcc aacccttgat ccgctcccac tgatcatcat cccgcagtgc
      121 atcgcaatcc atcaccgaac tccaaaagcc agtgttgaac ctgatttgac gccccgcggg
      181 aatcccaaaa acgtaaatcg tcaccacagc ctaggcgcag acatagaagc tcacttcggc
      241 tgacaacgtc cgctaccgat atccgaccgc taaccctctt cgccagacgg ggtcgccttg
      301 ccgcttacgg tgcatcgttg agccataaac agcatcgccc cctacacgac caagtggctg
      361 gtggcaggat gtattgtcat gtaaacaaaa tgctgtaatt tgtgatgagt acccgcaatc
      421 aaataatcct agccgtagca ttcttattgt tcaattaagc tcgtcgtcat aagaagaaaa
      481 aattgtgata aaataattga ttccgtagtt cattgagaag caagattgga ccccaagccg
      541 tcaaatgtcg taagtttcgg tgtccgtctt taatataatt gtttgttcaa tatttctaat
      601 aatacaaacg taagtatgac gcgttatact ttattaatta aaatcacgat ttttacaata
      661 tattgagaat tcccgttatt gaaaatgcga tggatgttaa tgatgctaat atttagttag
      721 ggttctgcga ggtttaattg gagcggtatt tatataatat agaaaatgaa ttgcaaaaac
      781 ataaaaataa ataatatatg tttgctacat accccactaa tgggcaaagg aaacttactg
      841 aaattaaata ttactaatat tcttgaccga atttgaccat ttcctctaca gcacgcccga
      901 tcgctaacac catatcgtcc gacccaaacg ggccctcgat ctgtattcca accggtagcc
      961 tgtcgggtgt caacgacact gggacgctca agcttggcag cccgatgtta ctgctaggat
     1021 ctaggtttcg aacgaaaatt ccaaaggtat cagtctcgac gccgttgtgt acaagcgtag
     1081 tctgcagccc tactggtttg gcggttaggg gaactgaagg aaataaaaca gcgtcaaggc
     1141 cgttggagga gtaataattc tcatatgttg cttttagcct gggcttgtag caccgaagcg
     1201 ccctccggta gaccgactct gaaataagtc cttttgatag ttgagattgc acgaggtcat
     1261 agacctgtgg atcctgaata ttctgtatca actcatcaaa cgtgtttcca atcccgtgaa
     1321 acgagagata ggccatgagc gctcggggaa actcataaat agcaactggc aagctcactt
     1381 tgcgggttag ctccgcaaca ttcggaatgt ccgcttccac gaacgtcatt tggttggtag
     1441 ccagacgtcg aattgctcgt tccgaggctg ccgcaacatg cggttcgaga ttatcaaaga
     1501 agtgggatcg aggcagaccc aaccgaagcc ccttgagtga catattttga gtaggacact
     1561 tgttgcgaat gatccgatcc aatagaaccg catcttcaac cgagcggaca atgaggccag
     1621 gagtgtctct cgacggactc accggcacaa tgccatcggt tggatagttg ccaaatgttg
     1681 gacgaaaacc aacaacccca catagtgccg ccggaagcct aacagaagcg ccggtgtcag
     1741 ttcctatgcc gcccaacatc aaattggctg ctaccgagac agcaacgccc ccactcgatc
     1801 caccagagat cagagcctgg ttccaagggt ttcgtgcggg accaaacgtc ttgttgtcat
     1861 ttgttatacc aaaagataac tcgtgcatat ttccagacgc cccaatcagt gcaccggctg
     1921 aatgaagtcg ctctactatc tttgccggaa tagctggctt gtgccgaagc agtccctgtg
     1981 tgccagcgga cgtcgggaat attccagtcg ctatgtttgc tttgaaacaa agggggatac
     2041 ccataaggcc caccccgccg gatccgttcc tatccacacg atctgcctca tttcttagat
     2101 gaagccagtc agttgcagcg aaggcgttca agtgggttgc agattccgaa cgggagacca
     2161 aagactcaac taggtcccga catgaatact gtttcctcct tagagctctc aacgtctgcg
     2221 aaattgatga tatttcaacc attttactag caagaccggc tctatttttt tgatattata
     2281 tgacgagtac ccttaattat aattttgtaa tacaattaca ccgccaatta cggggttaaa
     2341 ttcaatgagt tgactttgcc cgacagtggg tcccttcaat tttgaatagg cgtgcaagga
     2401 tactgttgca gattgaagac ttttcttccg cacaaccctc cagaccagct ctgcaaattg
     2461 tcttcgtggg acctacacta ttaatcaaaa tgatttttcg gttaaaagtt acttctataa
     2521 aagatgcttt aagcacagag cagaaacagc atcgaaatat ttcttatttt caatggctaa
     2581 ttttttctat tctagaatca caaacagaag ctactccacc aagaacttgc tcaacattga
     2641 agataaaggt cgcttgaaag atgagctcga gaagacgcgg caaaccaaca tttgcgagat
     2701 ttgcttgcat cctagggggc acagagcaag cgtctgtcga caaatattga tggggttcgc
     2761 ttacacaagc aaaaccgtgc tggaaggttt actttcgaca atgcctcatg atgacgcccc
     2821 gcttgggaaa atcttcgtaa cggatttgcc gccctatgat gagcaagtcc ctcaagtcct
     2881 gctgatgcaa gctgcagcac tagtcacaag ctacgaatat tcctttgaag atttagcata
     2941 tttcttggtc ctaccgctgc agatggctct aatgcaaaag tctccaagtc ttgggaagga
     3001 ttttccggta atttctggtt attcgatcac aaaagacagt gtgcactctc ctgtcgcgtt
     3061 tggaagaaat ttgatgccta ggggcgtcag ttgtgagttt cctcaaatcg atgttctcta
     3121 cgactatcgc ggatttctcg agggaggagc ttttagcgaa ggggtcacct ctttccctaa
     3181 agaaactaaa aaaccaaaag ttgccgtcat tggagccggt atttcaggat tggtttccgc
     3241 aacacttttg ctgcgaaacg gtatcgacga cgttaccata ttcgaagcca aaaacgtggt
     3301 gggaggtaga gcccacacac acttcttcaa aggcgaaccg tcggtctgcg cggaacttgg
     3361 tgcaatgagg tttcctcgca gccaagcttg cttgttctac ctgctggaat atctaggaat
     3421 caatgcaatg actaaatttc caaatccagg aacggttgat accggcttgt actaccgcgg
     3481 gagaagctac aactggaaag ctcatagcct ccccccagca attttcaatc gagttcataa
     3541 aggctggcgt acattcttgc acgcgggttt tgtagatggt gttgcagcat tcgcgtcccc
     3601 gtttacacta actgagtgtc tccggcttcg taactatgag ttcgcttcct cgctttggca
     3661 gaagtggctg gatgccttca gcagcgaaac attttcgtcc ggaatagaga gaatctttag
     3721 gggtgctcac ccaccaggtg gggagaaatg gaccagagat gtcgacatgg agctattcaa
     3781 agagctcgga gtggggtcgg gcggattcgg tccggtattt gggtgcggct tcatcgagat
     3841 attgcggttg atagtgaatg gctatgaaga caatgtaatg ttgctgcttg atggaataga
     3901 ggaaatccca cgaaggctat ctcagcagaa agttgggtca tattcaatcc gggatcggat
     3961 aatccacaaa gaggtgaagg agataattag gaccgagtca ggaatctcgc tagctattgg
     4021 cgaaggaatg catgcaacct ttgatcgcgt gatcgtaaca tctggcttta cgaatattca
     4081 gttgaggcat ctcttaacca atgatgacag ctttttttct tacgacgtta accaagcgat
     4141 cgagaacagc cacatgacag gctcatcaaa gctgtttgtt ctcacgcaga ataagttctg
     4201 gaaagctgaa gaactccctt cctgcatact gacaaccggt gttgccaaag cagtttactg
     4261 tctggattac gagcctgata aaccgagcgg aaaaggcctt gtacttttga gctacacttg
     4321 ggaagacgat tcccataaac tcctgacatt cgataaagga gaaagatttc aaatcttgaa
     4381 gcgcgatctc gcaaaatcat atcctagatt tgcggatttg cttgagccgg ctgatgggga
     4441 ttacgataac aacatcatcc agcacgactg gattctggac ccatatgcag gcggtgcttt
     4501 caagttaaat cgccgatgcg aagacgtcta ttcaaaaagg cttttctttc agccgcttcg
     4561 actgaacgga gagcctgatg gacgtgtctg tttggccggg tgcagttgtt ccttttctgg
     4621 cggatgggtg gagggcgcta tccagactgc atgtaacgct gcgatggcaa cgattagaga
     4681 tgctggcggc ttaattagcg gcgataaccc cctgacaaac gagttcgtta actatcatta
     4741 ctagagtagt gtctacctgt agacataaaa aggatgctat aacgcataac gttacgtttt
     4801 ttatttcgta taatgtaata aaatattcta gttatgtgta tatgtgaaaa atagcgaaaa
     4861 taattgcaaa ttagagtagt aagtattata atggatggta ccaaacgcaa atttaattgc
     4921 aaaagtattt cagagcgata atggaatgct tattaaagca ttaatacatc ttctagcaat
     4981 acttcgaaat gtatttatat tatggaattt tttttaaaat ttgggttgat taatgctagc
     5041 gtcagctatt tagcgagaaa gaatttggtc aaaggtcgcc tgtctacctg ctccaaagtc
     5101 aaatgttgaa acagggcaat agcaaacaat tacaacgttg aattctcctg gaagacaacg
     5161 gcgcccttat tgtgtaagtc ttgacaggat atatggtgat gtcacgtgca tccgtgcatc
     5221 cgtgcatccg tgcatccgtg catccgtgca tccgtgcatc cgtgcatccg tgcatccgtc
     5281 gcacccctcc ggcgttgctt gcaattaccc acatttgagt gtcgccgcat ttcggttcca
     5341 aggcgaatat ctgtcagccg actgaggccg cgccagacaa ccgtatttcc cggatgcgga
     5401 tctgatgttc ttgcaaggta accgcccaaa cgagcgagtt tgatgagata gaagtttaac
     5461 gatctcagcg tcgcctgcct gttgcctgcg ttgctgacca tttgatcgag gatatc
//