From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Lactococcus lactis histidine biosynthesis operon.
Date: 6 Nov 92 18:30:46 GMT
Distribution: bionet
Checksum: 11152 24

LOCUS       LACHISBIO   11208 bp ds-DNA             BCT       02-NOV-1992
DEFINITION  Lactococcus lactis histidine biosynthesis operon.
ACCESSION   M90760
KEYWORDS    amidotransferase; aminotransferase; cyclase; dehydratase;
            hisA gene; hisB gene; hisC gene; hisD gene; hisF gene; hisG gene;
            hisH gene; hisIE gene; histidine biosynthesis;
            histidinol dehydrogenase; hydrolase; isomerase;
            phosphoribosyl-ATP synthetase; pyrophosphate phosphohydrolase.
SOURCE      Lactococcus lactis (sub_species lactis) (library: NCDO2118) DNA.
  ORGANISM  Lactococcus lactis
            Prokaryotae; Firmicutes; Firmibacteria; Gram-positive cocci;
            Streptococcaceae.
REFERENCE   1  (bases 1 to 11208)
  AUTHORS   Delorme,C.C., Ehrlich,D.S. and Renault,P.P.
  TITLE     Histidine biosynthesis genes in Lactococcus lactis subup. lactis
  JOURNAL   J. Bacteriol. 174, 6571-7679 (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     terminator      10851..10878
                     /note="putative"
     CDS             2..637
                     /function="unknown"
                     /note="ORF 1"
                     /partial
                     /note="putative"
                     /codon_start=1
                     /translation="NSDSHQAINFFADEIKGTSSEKALTYQLRHTMSTAVYSEKNTKH
                     FGLAATNYTHFTSPIRRYPGFNHPSFIAFISSDHSNRTKDEWKERLPEIASHSSDMEH
                     RAVVTERIIDAMKKAEYMSERIGEVYTGTITGLQKFGIFVALDNTVEGLIRVPNLHTG
                     TTEELEFDEEASIFTGKKSETVYQIGQEIKIRVIAANKRKGTVDFEQIAPE"
     CDS             1077..2159
                     /function="aminotransferase"
                     /standard_name="HisC"
                     /evidence="EXPERIMENTAL"
                     /note="HisC"
                     /gene="hisC"
                     /codon_start=1
                     /translation="MSWQNNSGSKSLYWQVKQPELTGMIKLNTNENPYPPTSVAQLFN
                     ERYKTKNLRLYPSTDAKSLRKKLAEYHHLEVEQVFIGNGSDEVLSLSFLTFFNSQSPL
                     LMPDITYSFYPIYCELYRIPFQKVPVDDDFKVSIKDYCIENGGIVIANPNAPTALALN
                     LKDIEEILKKNQNSIVLIDEAYIDFGGETCLPLLKKYDNLVVVQTFSKSRSLAGIRLG
                     VAYGSAEAISHLYDVKNSFNSYPIDSLAQIIGEASLMDEHYFQKNIQKIIKTREVFKD
                     NLVNLGFEVTDSKANFVFVHHPKVKAEDLFKALYEAKIIVRHWNQPRIDDWLRITIGT
                     NKEMNKVIEFLKGYLKKNEEIDEWKK"
     CDS             2147..3133
                     /function="unknown"
                     /note="ORF 3"
                     /note="putative"
                     /note="HisC"
                     /codon_start=1
                     /translation="MEKINYLLPEESAEMTLNQVKSLRQIEGRLRKLFSLKNYQEVMP
                     PSFEYTQLYTALESNGKTFNQEKMFQFINHEGQSITLRYDFTLPLVRLYSQIKDSTSA
                     RYSYFGKIFRKEKRHKGRSTENYQIGIELFGESADKSELEILSLALQVIEQLGLNKTV
                     FEIGSAKFFQRLCHLADGSTELLTELLLKKDLSGLNAFIEKNNFSKELRELLKEIFIT
                     NELSRLENLVTNTKDDVLISSFDQLKEFSEKLSMIKPIIIDLGMVPKMDYYTDLMFKA
                     YSSAANQPILSGGRYDQLLSNFQEEAVAIGFCCHMDTILKALERQELEEDND"
     CDS             3126..3752
                     /function="phosphoribosyl-ATP synthetase"
                     /standard_name="HisG"
                     /evidence="EXPERIMENTAL"
                     /note="HisG"
                     /gene="hisG"
                     /codon_start=1
                     /translation="MIKIAITKGRIQKQVTKLLENADYDVEPILNLGRELQIKTKDDL
                     QIIFGKANDVITFLEHGIVDIGFVGKDTLDENDFDDYYELLDLKIGQCIFALASYPDF
                     SNKNFQRRKRIASKYPRVTKKYFAQKQEDIEIIKLEGSVELGPVVGLADAIVDIVETG
                     NTLSANGLEVIEKISDISTRMIVNKSSFKFKKDKIIEMVERLEDAQTN"
     CDS             3736..5031
                     /function="histidinol dehydrogenase"
                     /standard_name="HisD"
                     /evidence="EXPERIMENTAL"
                     /note="HisD"
                     /gene="hisD"
                     /codon_start=1
                     /translation="MLKQIDYQGKLEEIAEKFQGRKTEVSKEVNKTVQQIVADIQKNG
                     DTALFNYAKKFDGYDVNTSNLLVTRMEREAGLEQIDEDYFRILRRTKSQIEEFHKHQL
                     GNSWNIFKENGVIMGQIARPLERVALYVPGGTAAYPSTVIMNAVPALLAGVKEIIMIT
                     PVKADGKVNPNILAAAEVCGIETIYKVGGAQGVAAVAYGTESIPKVDKIVGPGNIFVA
                     TAKKICYGVVDIDMIAGPSEVLVIADKTAKPKYIAADLMAQAEHDKLASAILVTTSEK
                     LVQQVDEELNRQVQNLERREIIESSIRNYGGAIVVKNIDDAFDVSNQLAPEHLEVLTS
                     EPLTQLPKIKNAGSIFIGEYTPEPLGDYMSGSNHVLPTGGTAKFYSGLGVYNFIKYLT
                     YSYYPKEVLADFKEDVETFAKSEGLTAHANSISVRFDEM"
     CDS             5082..5873
                     /function="unknown"
                     /note="ORF 6"
                     /note="putative"
                     /note="HisD"
                     /codon_start=1
                     /translation="MDFKILNKKNSREKNMTKQENYYAEVFEKPWGRMFYDLLFPQLL
                     PNLTKDSKILSFGSGFGRTETFLEEQGFEVTGYEPDVEKLEMMSDQTFRQLTGTFDDF
                     AETVKNERYDVILIHNVLEYVLDRKVVLELLLSLLTDGGTLSIVKHSKYGSMIEMAAG
                     RDNPQAALDVYENEAVASHNHGDILVYDDDWLTDFVANYKLKLQEKFGIRHFYGISQN
                     AEIKETENWYQPMLKLEQKVAKDQTLYPVARLHHLIFKKTKENLL"
     CDS             5870..6472
                     /function="dehydratase"
                     /standard_name="HisBd"
                     /evidence="EXPERIMENTAL"
                     /note="HisBd"
                     /gene="hisB"
                     /codon_start=1
                     /translation="MTRISHITRNTKETQIELSINLDGTGQADISTGIGFLDHMLTLL
                     TFHSDFDLKIIGHGDHETVGMDPHHLIEDVAIALGKCISEDLGNKLGIRRYGSFTIPM
                     DEALVTCDLDISGRPYLVFHADLSGNQKLGGYDTEMTEEFFRALAFNAGITLHLNEHY
                     GQNTHHIIEGMFKSTARALKQAVSIDESKVGEIPSSKGVL"
     CDS             6469..7257
                     /function="unknown"
                     /note="ORF 8"
                     /note="putative"
                     /note="HisBd"
                     /codon_start=1
                     /translation="MTNLKELRINIEKFPEALHNTLKDAKIYDSSSSPEAQVLFIDKK
                     DGYYLKIASSKTLEREAEMTAYFQKKKLGLGYISYLSDQSQDFLLKKKIQGENYLAKQ
                     YLNNPKRLCDNLAENLRFLHEQNFEDCPILDHSERYLKKVEKNASINNSNLDFVNNYN
                     IRTTEEAYDYIENKKLLLRNDTLLHGDYCLPNIILDNWKFKGFIDLDCAGVGDRHIDL
                     FWGAWTLNFNIGTDQYRDRFFDAYGRDRIDVDRLKLVGCCEVFG"
     CDS             7296..7904
                     /function="amidotransferase"
                     /standard_name="HisH"
                     /note="putative"
                     /note="HisH"
                     /gene="hisH"
                     /codon_start=1
                     /translation="MKKIVIIDYNIGNLQSVQAAFLRLGQETVISRDLEEIRKADALI
                     LPGVGAFPTAMNNLKKFNLIELIQERAAAGIPILGICLGMQVLFEKGYEIEERQGLGL
                     LKGEVIPIKTNEKIPHMGWNQLNLAKTSPTTHYLSGNDEVYFVHSYQATCPDDELIAY
                     TTYGEVKIPAIVGKNNVIGCQFHPEKSGEIGRKILKAFLEEI"
     CDS             7905..8624
                     /function="isomerase"
                     /standard_name="HisA"
                     /evidence="EXPERIMENTAL"
                     /note="HisA"
                     /gene="hisA"
                     /codon_start=1
                     /translation="MKIIPAIDLQNGEAVRLYKGDYDKKTVYSKNPLEIAQKFERMGA
                     TDLHLVDLDGAKIGQTRNLELVRKIKDETRLKIEIGGGIRDFDTVRMYLEQIGVERVI
                     LGTAAVEKPDFLKELLIKYGPSRIVVGVDIREGFVSTSGWLEKTSLPYLSFLKKLERI
                     GVKTTIITDISKDGTLTGPNFKLYDEISKENSLNVIISGGVKDNSDIQRATRSDFYGI
                     IVGKAYYEGKINLEKEFRNAN"
     CDS             8614..9348
                     /function="cyclase"
                     /standard_name="HisF"
                     /evidence="EXPERIMENTAL"
                     /note="HisF"
                     /gene="hisF"
                     /codon_start=1
                     /translation="MLTKRIIPCLDIKNGKVVKGINFVGLREIGDPVELAKIYEEQCA
                     DEIVFLDITASFEEREIIGELIGRAARELSIPLTVGGGIRSIDDFRRILARGADKVSV
                     NSAAIENPELIRQAANEFGVQCVVVAIDAKKRADHRGYDVYIKGGRENAGLDLVDWAK
                     KCERLGAGEILLTSMDKDGTKTGYDLEMLNDVCTAVNIPVVASGGCGKISDIVEVFQN
                     TRSDAALFASLFHYGEEQLMKLKTNL"
     CDS             9389..10027
                     /function="hydrolase, pyrophosphohydrolase"
                     /standard_name="HisIE"
                     /evidence="EXPERIMENTAL"
                     /note="HisIE"
                     /gene="hisIE"
                     /codon_start=1
                     /translation="MRPDFHKQELIPVIVQDYQTNQVLMLAYTNEVAFEKMLETGETW
                     FWSRSRQKLWHKGEESGHFQKIKGMRLDCDQDTLLVFVEQIGNACHTGAYSCFYDELI
                     PFDDSDIFSELEKQIIDRKLHPVEKSYTNYLLGEGIDKVLKKVGEEASEVTIASKNSD
                     KGELLGEIDDLLYHLFVLMNQQGISLEEVRQKAKERHQLEGNKKEFHTRTAD"
     CDS             10028..10837
                     /function="unkwown"
                     /note="ORF 13"
                     /note="putative"
                     /note="HisIE"
                     /codon_start=1
                     /translation="MKKLDYHFHSHFSADSEELPRKHVIEAIAYGLEEICFTEHRDFY
                     FPGMDFSLNLPEYFQEINRLQAEFKDEINIKIGLEMGIDLRFKSEINQFIDSAPFDFV
                     IASVHEIGDIEVYDGTEFYLQKTKEEAQREYLLACLDVVQNFETYNSFGHLDYVARYG
                     PYTDKSITFAENREILFEILRALASKGKALEINTRLFDDPKTGQFYSDLLINFKRLGG
                     KFITLGTDSHIAKRDWLSIHKARTLIKKAGFRELATFSGMKIDKNKKSIKE"
BASE COUNT     3864 a   1636 c   2239 g   3469 t
ORIGIN      
        1 gaattcagat tctcatcaag ccattaattt ctttgctgat gaaattaaag gaacaagttc
       61 tgaaaaggca ttgacttatc aacttcgaca tacgatgtca acagcggttt actctgaaaa
      121 aaatacaaaa cattttgggc tcgcagcgac taactatacg catttcacaa gcccaattag
      181 acgttatcct ggatttaatc atccatcgtt tattgcattt atatcctctg accattctaa
      241 ccgtactaaa gacgagtgga aagaacgttt acctgaaatt gctagccatt cttctgatat
      301 ggaacaccgt gcggtcgtca ctgaacgaat tattgatgca atgaaaaaag cagaatatat
      361 gtcagagcgg attggagaag tttacactgg tacaattaca ggcctacaaa aatttggaat
      421 ctttgttgca ttagataata cagttgaagg tttaattcgt gtacctaatt tacatactgg
      481 gactacggaa gaacttgaat ttgatgaaga agcaagtatt tttacaggaa aaaaatcaga
      541 aacagtctat caaataggac aagaaataaa aattcgtgtt attgctgcta ataaacgaaa
      601 aggaacagtt gattttgaac aaattgctcc tgaataaatc cagtctacta ttatgtcgaa
      661 taaataaagg actaataaga taaattaaac tattgaaatt taaatatttt tattatgcta
      721 aataacaaca tagatatttt attaaaataa atgttgactt ttaaaaacat aagtattata
      781 attcagataa ttaagaattg acaaacaaga gatgaaagag tagtcttagg tatcagttga
      841 gagagccttg ttgctgagaa aaggtctgat aatgggatga accacactca tcgctatctt
      901 actcacatta ttatgtgagc gttagctgcg ttaatgctaa gttgttataa cttaccgagg
      961 tcacttttgt gaaaaggtga taaattaagg tggaaccacg attaaaaccg tcctttaagc
     1021 caagtgcttt taggcgtttt tttatattat ctttctattc tcgaaaggag aaatctatga
     1081 gttggcaaaa taactcgggc agtaagtccc tatattggca ggtgaaacaa cctgaactta
     1141 ctggcatgat caaattaaac actaacgaaa atccatatcc gcccactagt gtagctcaat
     1201 tatttaatga acgttataag acaaaaaatt tgcgtcttta cccaagtact gacgcgaaaa
     1261 gtttaagaaa aaaattggcc gaatatcatc atttagaagt tgaacaggtt tttattggaa
     1321 atggctctga cgaagttttg tcacttagtt ttctgacttt ttttaatagt caaagccctt
     1381 tattaatgcc tgacattact tattcttttt accctattta ttgcgaactc tatcgaattc
     1441 cttttcaaaa agttcctgtg gatgatgatt ttaaagtttc aataaaagat tattgtattg
     1501 aaaatggtgg aattgtgatt gcaaatccta atgctccaac ggctttggcg ctaaatctta
     1561 aagatataga agaaattctg aaaaaaaatc aaaactcaat tgtcttgatt gatgaagcct
     1621 atattgattt tggcggtgaa acatgtcttc ctttgcttaa aaaatacgat aatttagtag
     1681 tggttcaaac tttttctaaa tcacggagtt tggcaggaat tcgtttgggt gtagcttatg
     1741 gctctgctga agcaatttct catttgtatg atgtgaaaaa ttcatttaat tcctatccaa
     1801 ttgatagttt ggcacaaatt attggagaag caagtttaat ggatgaacat tattttcaaa
     1861 aaaacattca gaaaatcatt aagacaagag aagtttttaa agataatctg gttaatttag
     1921 gatttgaagt gactgattca aaggctaact ttgtttttgt tcatcatcca aaagtaaaag
     1981 cagaagacct ttttaaggcg ctttatgagg caaaaattat tgtcaggcat tggaatcaac
     2041 cacggattga tgattggtta cggataacta ttggaactaa taaagaaatg aacaaagtga
     2101 ttgaattttt aaaaggctat ttaaaaaaga atgaggaaat tgacgaatgg aaaaaataaa
     2161 ttatctgctt cctgaagaat cggcagaaat gaccttgaat caagttaaaa gtctacggca
     2221 gatagaaggg cgtttaagaa aattatttag cttgaaaaat tatcaggaag tcatgccccc
     2281 aagctttgaa tatacacaac tctatacagc acttgaaagt aatggaaaaa cttttaatca
     2341 agaaaaaatg tttcagttta tcaatcatga gggacaatca attacacttc gttatgattt
     2401 tacacttcct ttagttagac tctattcgca aatcaaggat tctactagtg cccgttattc
     2461 atattttgga aaaatattta gaaaagaaaa acggcataaa ggtcgttcaa cggaaaatta
     2521 tcagattggt attgaacttt ttggtgaatc agccgataag tcagaattag aaattcttag
     2581 tttagccctt caagtgattg aacagttagg tttgaataaa acggtctttg aaataggctc
     2641 agcaaaattt tttcaacgtt tatgtcattt agctgacggt tcaacagagt tacttacaga
     2701 acttttactc aagaaagatt tgagtggtct taatgctttt atcgaaaaaa ataatttttc
     2761 taaggaatta agagaacttt tgaaagaaat atttattact aatgagttat caaggttgga
     2821 aaatttagtg acaaatacaa aagatgatgt gctcatttct tcctttgatc agctcaaaga
     2881 attttcagag aaactttcaa tgattaaacc gattatcatt gatttgggaa tggttcctaa
     2941 aatggattat tatactgatt taatgtttaa agcttacagt tcagcagcga atcaacctat
     3001 tttatcaggt ggaagatatg accaactatt aagtaatttt caagaagagg cggttgccat
     3061 tggtttttgt tgtcatatgg atactatttt aaaggcactg gaaagacaag aattggagga
     3121 agacaatgat taaaattgcc ataactaaag gtcgaatcca aaaacaagtc accaaacttt
     3181 tagaaaatgc ggactatgat gttgaaccaa ttctaaatct aggacgtgaa ttacaaatta
     3241 aaacgaaaga tgatttacaa atcatttttg gaaaagctaa tgatgtcatt acttttttag
     3301 aacatggaat tgttgatatt ggctttgttg gtaaagatac gcttgatgaa aatgattttg
     3361 atgattatta tgaattattg gatttaaaaa ttgggcagtg tatttttgcc cttgcttcct
     3421 atcctgactt ttcaaataaa aattttcaaa gacgtaaacg aattgcttca aaatatccaa
     3481 gagtgacaaa aaaatatttt gctcaaaagc aagaagatat tgaaattatc aagttggaag
     3541 gttctgttga gcttggacca gttgttggtt tagctgatgc aattgttgac attgtcgaaa
     3601 caggaaatac tttatctgca aatggtttag aggtcattga aaaaatcagt gacatttcaa
     3661 cacggatgat tgtcaataaa tctagtttca aatttaaaaa agataaaatt atagaaatgg
     3721 tggagaggtt agaagatgct caaacaaatt gattatcaag gaaagcttga agaaattgct
     3781 gaaaaatttc aaggtcgaaa aacagaagta tcaaaagaag ttaataaaac tgttcaacag
     3841 attgtagcag acattcaaaa aaatggagat actgccttat ttaactatgc caaaaagttc
     3901 gacggttatg atgtgaatac tagtaattta ctggtcacgc gcatggaacg tgaagcagga
     3961 ctagaacaaa ttgatgagga ttattttaga attcttagac gcaccaaatc acaaatcgaa
     4021 gaatttcata agcaccaact gggaaattca tggaatattt ttaaggaaaa tggtgttatc
     4081 atgggacaaa ttgcgcgtcc tctggaacgt gttgctctct atgttcccgg aggaacggct
     4141 gcatatccct caacagtcat tatgaatgct gttccagcgc ttttagcagg cgtcaaagaa
     4201 attattatga ttactccagt taaagctgat ggaaaagtaa atccaaatat tttagcagct
     4261 gctgaagttt gtggaataga aacaatctat aaggtgggtg gagcacaagg cgttgctgcc
     4321 gttgcgtatg ggacagaatc tattcccaaa gttgataaga ttgtcggacc cggaaatatt
     4381 tttgtggcta cagctaagaa aatctgttat ggggtggtag atattgatat gatagccggt
     4441 ccgtcagaag ttctagttat tgctgacaaa actgccaagc caaaatatat cgctgctgat
     4501 ttaatggcgc aagcagaaca tgataaactt gcgtcagcga ttctagtgac gacttctgaa
     4561 aaacttgttc aacaagtaga tgaggaatta aatagacaag ttcaaaattt ggaacgtcgt
     4621 gaaatcattg aaagttccat caggaattac ggtggagcca ttgttgtaaa aaatattgat
     4681 gatgcctttg atgtttccaa tcagctggct ccagaacatt tagaagtttt gactagtgaa
     4741 cctttaaccc aacttccaaa aatcaaaaat gctggctcaa tttttattgg agagtatacg
     4801 ccagaaccgt taggcgacta tatgtcagga agcaatcatg tcttaccaac tggaggaaca
     4861 gccaaatttt actctggttt gggtgtttat aattttataa aatatttgac ttatagctat
     4921 tatcctaaag aagttttggc tgactttaaa gaggatgttg agacatttgc aaaatcagaa
     4981 ggattgacgg ctcatgctaa ctcaatttct gtgagatttg atgaaatgta atacttgaga
     5041 caagattttc aaaatttatt ggtcaaggcg tgaggagcca catggatttt aaaatattga
     5101 ataagaaaaa tagtagggag aaaaacatga caaaacaaga aaattattac gcagaagttt
     5161 tcgaaaaacc atggggtcgg atgttctatg acttactttt tccacagctc ctaccaaatt
     5221 tgacaaaaga ttcaaaaatt ctgagtttcg gctctggatt tggacggacg gaaacatttt
     5281 tggaggaaca gggatttgaa gtcacgggct atgagcctga tgtagaaaag ctcgagatga
     5341 tgtctgacca aacttttcgt cagttgacag gaacttttga cgactttgca gaaactgtta
     5401 aaaatgagcg ttacgacgtg attctcattc acaatgtttt agaatacgtc cttgaccgaa
     5461 aagtcgtgtt ggaattactc ttgtcacttt tgacagatgg cggcacgctt tctattgtca
     5521 agcacagtaa gtacggtagc atgatagaaa tggcagcagg acgtgataat ccgcaggcag
     5581 cgcttgatgt ttatgaaaat gaagctgtcg cttctcacaa ccacggcgat atcctagttt
     5641 atgacgatga ttggctgaca gattttgtgg caaattacaa actgaaactc caagaaaaat
     5701 ttggaattcg tcatttttac ggtatttcac aaaacgcaga aatcaaagaa acagagaact
     5761 ggtatcaacc catgcttaag ttagagcaaa aagtagcgaa agaccaaacg ctgtatccag
     5821 tcgcacgatt acatcatttg atatttaaaa aaactaagga gaatctgtta tgacacgcat
     5881 atcacacatc acgcgtaata ccaaagaaac acaaatcgaa ctttccatca atcttgacgg
     5941 cacaggtcaa gcggacatta gtacaggtat tggttttctc gaccacatgc tgacacttct
     6001 cacctttcac agcgattttg acttaaaaat cataggacat ggggatcatg aaacagtagg
     6061 gatggacccg caccatctca ttgaagatgt tgcgattgct cttggcaaat gtatcagcga
     6121 agatttaggt aataagctcg gtattcgacg ttatggaagt tttaccattc caatggatga
     6181 agctttggtg acttgtgatt tagatattag tggacgacct tatttggtat ttcatgctga
     6241 tttatcagga aatcaaaaac ttggtggcta tgatacagaa atgactgaag aattttttcg
     6301 tgcccttgct tttaatgctg ggattacgtt acatctgaac gaacattatg ggcaaaatac
     6361 gcatcatatt attgaaggca tgtttaaatc tacagcaaga gcgctaaaac aagctgtaag
     6421 tattgatgaa tcgaaagttg gagaaatacc gagcagtaag ggagtgttat gactaatcta
     6481 aaagaattaa gaattaatat tgagaaattt cctgaagcac ttcacaatac gctaaaagat
     6541 gcaaaaatat atgatagcag ttcctctcct gaagctcaag tgttattcat tgataaaaaa
     6601 gatggctatt atttaaaaat agcttcatca aaaactttag agcgagaagc tgaaatgact
     6661 gcttattttc aaaagaaaaa gttaggttta ggatatattt cttatttatc agaccagtca
     6721 caggattttt tactcaagaa aaaaattcaa ggagaaaatt atttggctaa acaatatctt
     6781 aataatccga aacgtctgtg tgataatctt gctgaaaatc tacgatttct tcatgaacaa
     6841 aattttgagg attgtcccat attagaccat tctgaacgtt atctgaaaaa agtcgaaaag
     6901 aacgcaagta taaataattc aaatctagat tttgtcaata attataatat ccgaacaaca
     6961 gaagaagctt atgattatat agaaaataaa aaattgctat taaggaatga tacgctttta
     7021 catggagatt attgtttacc aaatataatc ctagacaatt ggaagttcaa aggttttatt
     7081 gatttggatt gtgcaggtgt tggcgaccga catattgacc tcttttgggg tgcatggacg
     7141 cttaatttta atattggtac tgatcaatat cgtgaccgat tttttgatgc ttatggtcgg
     7201 gatagaatcg atgttgatcg tttgaaattg gttggctgct gtgaggtttt tggttaaatt
     7261 gaattttagt atattttgat attgaaagga gcattatgaa aaaaattgtt atcatcgact
     7321 acaatattgg aaatcttcaa agtgtacagg ccgcattttt acgattgggg caagaaacag
     7381 tgatttcaag agatttagag gaaattcgta aagcagatgc acttattctt ccaggagttg
     7441 gggcttttcc tacagcaatg aataatttaa aaaagtttaa tttgattgaa cttatacaag
     7501 aacgagctgc tgctggaata ccaatattgg gaatttgttt agggatgcaa gtgctttttg
     7561 aaaaaggata cgagatagaa gaaagacaag gtcttggact tttaaagggt gaggtaattc
     7621 caatcaaaac taatgagaaa attccgcata tgggatggaa tcaattaaac ctggctaaaa
     7681 ccagtccaac aactcattat ttgtctggta atgatgaggt ctattttgtc cattcttatc
     7741 aggcgacttg tcctgatgat gaactcattg cctacaccac ttatggcgaa gtaaaaattc
     7801 cggcaattgt tggaaaaaat aatgtgatag gctgtcaatt tcaccctgaa aagagtggag
     7861 aaattggcag aaagatacta aaagcattct tggaggaaat ttaaatgaag attattccag
     7921 caattgattt gcaaaatggt gaagccgtgc gtctctacaa aggagattat gataagaaaa
     7981 cggtctattc aaaaaatccc cttgaaattg ctcaaaaatt tgaaagaatg ggagcgactg
     8041 acctccattt ggttgattta gatggtgcta agataggaca aactcgtaat ttagagcttg
     8101 tgcgaaaaat aaaagatgaa acaagattga aaatcgaaat tggtggtgga attagagatt
     8161 tcgatacagt tagaatgtat cttgaacaaa ttggtgtgga acgagtgatt ttaggaaccg
     8221 cagcggtaga aaagcctgat tttcttaagg aattattaat taaatatggt ccaagcagaa
     8281 tcgttgttgg agttgatatt agagagggtt ttgtatcaac aagtggttgg ttagaaaaaa
     8341 caagtcttcc ttacctgtca tttttaaaaa aattagagag aataggggtt aaaaccacta
     8401 ttattactga tatctccaaa gacggaacac tgacaggtcc aaattttaaa ctttatgatg
     8461 aaatttcaaa ggaaaattcc ctaaacgtga ttatttctgg aggtgtaaag gataattctg
     8521 atattcaacg tgcaactcgt tctgacttct atggaattat cgttgggaaa gcttactatg
     8581 agggaaaaat taatcttgaa aaggagttca ggaatgctaa ctaaaagaat catcccttgt
     8641 cttgatatta aaaatggtaa ggttgttaaa ggaatcaatt ttgtgggttt aagagaaata
     8701 ggtgatccag tggaattggc caaaatatat gaagaacagt gtgcagatga aattgttttt
     8761 cttgatatta cagcatcttt tgaagaacgt gaaattattg gtgaattaat tggtcgggcg
     8821 gcgcgtgaac tttcaattcc attaaccgtt ggtggaggaa ttcgttcaat tgacgatttt
     8881 agaagaattc ttgctagagg agctgataaa gtatccgtca actcagctgc aattgaaaat
     8941 cctgaactta ttcgtcaagc ggctaacgaa tttggcgttc aatgtgttgt tgttgctatt
     9001 gatgctaaaa aacgagcaga ccatagggga tatgatgtct atatcaaagg tgggcgtgaa
     9061 aatgcaggtc ttgacttagt tgattgggct aaaaagtgtg aaagattagg agcaggcgaa
     9121 attttactaa cctcaatgga taaggatgga acaaagacag gctacgattt ggaaatgctg
     9181 aatgacgttt gtactgctgt taatattcct gttgtagcaa gtggcggttg tggcaagatt
     9241 tcggacattg ttgaagtttt tcaaaataca agaagcgatg ctgccctttt tgcttcatta
     9301 tttcattatg gcgaagaaca gttgatgaag ttaaagacga acttataaaa aataatattc
     9361 cagcaagaat tattaagaag gagacattat gagaccagat tttcataagc aggaacttat
     9421 tcctgttatt gttcaagatt atcaaacaaa tcaagtatta atgcttgcct atacaaatga
     9481 agtggctttt gagaaaatgc ttgaaactgg tgagacttgg ttttggtcac gttcacgaca
     9541 aaaactttgg cacaaaggag aagaatcagg tcattttcaa aaaataaaag gaatgcgttt
     9601 ggattgcgac caagatactt tactggtttt tgttgaacaa attggtaatg cttgtcatac
     9661 tggtgcttac tcttgttttt atgatgaact tattcctttt gatgactctg atatttttag
     9721 tgagttggag aagcaaatca tcgaccgaaa gttacatcca gtcgaaaaat cttatacgaa
     9781 ttatttgctt ggagagggca ttgataaagt attaaaaaaa gttggtgaag aagcaagtga
     9841 agtgactatt gctagtaaaa attcagataa aggagaactt ttaggagaaa ttgatgattt
     9901 actttatcat ttgtttgtat taatgaatca gcaaggtatt tctctagaag aagtacggca
     9961 aaaagcaaaa gaacggcatc agctagaagg aaataagaag gaatttcata caaggacggc
    10021 agattaaatg aaaaaattag attatcattt ccactctcat ttttcggctg atagcgagga
    10081 gcttcccaga aagcatgtga tagaagctat agcatatggt ttagaagaaa tttgttttac
    10141 agagcatcga gatttttatt ttccaggaat ggatttttca ctaaatctac ctgagtattt
    10201 tcaagaaatc aatcgattac aagcggaatt taaagatgaa attaatataa aaatcggttt
    10261 ggaaatggga attgatttac gttttaaatc agaaattaat cagtttattg attcggctcc
    10321 ttttgacttt gtgattgcct ctgttcatga aattggagat attgaagttt atgacggaac
    10381 agaattttat ctacagaaaa caaaagaaga agcacaaaga gaatatcttt tagcttgttt
    10441 agatgttgtt caaaattttg agacttataa ttcttttggc catttagatt atgttgctag
    10501 atatgggcct tacacggata aatcaataac gtttgcagag aatcgggaga ttttatttga
    10561 aatcttgcgg gctttagctt caaaaggaaa agcattagaa ataaatacta gattgtttga
    10621 tgatccgaaa actggccaat tttatagtga tttattgatt aattttaaaa gattaggtgg
    10681 aaaatttata actttaggaa cggatagtca tatagcaaag cgtgattggc tttcaattca
    10741 taaagcaaga actttaatta aaaaagctgg ttttcgcgaa ctagcaactt ttagtgggat
    10801 gaaaattgat aaaaataaaa agtctattaa agaataatag actttttata ttatgatgct
    10861 aaatctttct caagtagcgc aagacgttct tgaacttctt gatttgatag ttggtgggct
    10921 ttgatgtaac ggttattggg atgaactcga cattcatgtg aacaagcacc aagatatttt
    10981 gcttcgtttt cctctgaagc taacatttgt ctgttacatt ctggatttcc gcaattgatg
    11041 taacgttcac atgggcttcc atcaaaccaa tcacgtccga caatgacatg ttctttttgg
    11101 ttgattggaa ctgcgattcg gctatcaaaa acatacattt gtccgtccca gagatctcct
    11161 tgaacttctg ggtcttttcc gtatgtggca attcctccgt gaagtatc
//