From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: S.pneumoniae epuA and endA genes for 7 kDa protein and membrane
Date: 3 Jan 93 22:22:19 GMT
Distribution: bionet
Checksum: 01783 4

LOCUS       STRENDA      1421 bp ds-DNA             BCT       23-DEC-1992
DEFINITION  S.pneumoniae epuA and endA genes for 7 kDa protein and membrane
            endonuclease
ACCESSION   X54225
KEYWORDS    DNA transport; endA gene; epuA gene; membrane bound protein;
            nuclease; terminator.
SOURCE      Streptococcus pneumoniae DNA.
  ORGANISM  Streptococcus pneumoniae
            Prokaryotae; Firmicutes; Firmibacteria; Gram-positive cocci;
            Streptococcaceae.
REFERENCE   1  (bases 1 to 1421)
  AUTHORS   Puyet,A., Greenberg,B. and Lacks,S.A.
  TITLE     Genetic and Structural Characterization of EndA A Membrane-bound
            Nuclease Required for Transformation of Streptococcus pneumoniae
  JOURNAL   J. Mol. Biol. 213, 727-738 (1990)
  STANDARD  full automatic
COMMENT     From EMBL    entry SPENDA;  dated 09-DEC-1992.
FEATURES             Location/Qualifiers
     RBS             123..126
     RBS             356..361
     stem_loop       1203..1233
     terminator      1203..1233
     -35_signal      1330..1335
     -10_signal      1354..1359
     RBS             1407..1411
     CDS             133..327
                     /gene="epuA"
                     /product="7 kDa protein"
                     /codon_start=1
                     /translation="MKMNKKSSYVVKRLLLVIIVLILGTLALGIGLMVGYGILGKGQD
                     PWAILSPAKWQELIHKFTGN"
     CDS             366..1190
                     /gene="endA"
                     /product="membrane nuclease"
                     /codon_start=1
                     /translation="MNKKTRQTLIGLLVLLLLSTGSYYIKQMPSAPNSPKTNLSQKKQ
                     ASEAPSQALAESVLTDAVKSQIKGSLEWNGSGAFIVNGNKTNLDAKVSSKPYADNKTK
                     TVGKETVPTVANALLSKATRQYKNRKETGNGSTSWTPPGWHQVKNLKGSYTHAVDRGH
                     LLGYALIGGLDGFDASTSNPKNIAVQTAWANQAQAEYSTGQNYYESKVRKALDQNKRV
                     RYRVTLYYASNEDLVPSASQIEAKSSDGELEFNVLVPNVQKGLQLDYRTGEVTVTQ"
BASE COUNT      461 a    252 c    331 g    377 t
ORIGIN      
        1 tgattttgac aggtttggta gcacagggag aaactgtggt cggtaaattg gttcacttgg
       61 atagaggtta ctacggtttc catgagaagt tggcgcagct aggtgctaag attcagcgga
      121 ttgaggcaaa tgatgaagat gaataagaaa tcaagctacg tagtcaagcg tttactttta
      181 gtcatcatag tactgatttt aggtactctg gctctaggaa tcggtttaat ggtaggttat
      241 ggaatcttgg gcaagggtca agatccatgg gctatcctgt ctccagcaaa atggcaggaa
      301 ttgattcata aatttacagg aaattaggct ggagaaccag cctttttcta aagataagga
      361 gaaatatgaa caaaaaaaca agacagacac taatcggact gctagtgtta ttgcttttgt
      421 ctacagggag ctattatatc aagcagatgc cgtcggcacc taatagtccc aaaaccaatc
      481 ttagtcagaa aaaacaagcg tctgaagctc ctagtcaagc attggcagag agtgtcttaa
      541 cagacgcagt caagagtcaa ataaagggga gtctggagtg gaatggctca ggtgctttta
      601 tcgtcaatgg taataaaaca aatctagatg ccaaggtttc aagtaagccc tacgctgaca
      661 ataaaacaaa gacagtgggc aaggaaactg ttccaaccgt agctaatgcc ctcttgtcta
      721 aggccactcg tcagtacaag aatcgtaaag aaactgggaa tggttcaact tcttggactc
      781 ctccaggttg gcatcaggtc aagaatctaa agggctctta tacccatgca gtcgatagag
      841 gtcatttgtt aggctatgcc ttaatcggtg gtttggatgg ttttgatgcc tcaacaagca
      901 atcctaaaaa cattgctgtt cagacagcct gggcaaatca ggcacaagcc gagtattcga
      961 ctggtcaaaa ctactatgaa agcaaggtgc gtaaagcctt ggaccaaaac aagcgtgtcc
     1021 gttaccgtgt aaccctttac tacgcttcaa acgaggattt agttccctca gcttcacaga
     1081 ttgaagccaa gtcttcggat ggagaattgg aattcaatgt tctagttccc aatgttcaaa
     1141 agggacttca actggattac cgaactggag aagtaactgt aactcagtaa aagataagcc
     1201 taaactccta tgtcacttat ggatgtagga gttcttttta ctagtttaag cagggctaga
     1261 acaggtacta agaaaaaata gcaacttcta aaactaactt ccagttttgg gagagagatg
     1321 gaagttactt tgagaagtta cggtaagaaa aaataatatg tattggatta aattttgtga
     1381 tataataaag gataagatac tattttagga gaagaactat g
//