From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Pseudomonas pickettii phenol/cresol hydroxylase (tbuD) gene,
Date: 31 Dec 92 01:47:21 GMT
Distribution: bionet
Checksum: 25674 6

LOCUS       PSETBUD      2191 bp ds-DNA             BCT       18-DEC-1992
DEFINITION  Pseudomonas pickettii phenol/cresol hydroxylase (tbuD) gene,
            complete cds.
ACCESSION   M98806
KEYWORDS    phenol hydroxylase; tbuD gene.
SOURCE      Pseudomonas pickettii (strain PKO1) DNA.
  ORGANISM  Pseudomonas pickettii
            Prokaryotae; Gracilicutes; Scotobacteria; Pseudomonadaceae.
REFERENCE   1  (bases 1 to 2191)
  AUTHORS   Kukor,J.J. and Olsen,R.H.
  TITLE     Complete nucleotide sequence of tbuD, the gene encoding
            phenol/cresol hydroxylase from Pseudomonas pickettii PKO1 and
            functional analysis of the encoded enzyme
  JOURNAL   J. Bacteriol. 174, 6518-6526 (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     promoter        19..45
                     /gene="tbuD"
     RBS             70..73
                     /gene="tbuD"
     mat_peptide     86..2095
                     /EC_number="1.14.13.7"
                     /product="phenol hydroxylase"
                     /gene="tbuD"
                     /evidence="EXPERIMENTAL"
                     /codon_start=1
     terminator      2141..2172
     CDS             83..2098
                     /gene="tbuD"
                     /product="phenol hydroxylase"
                     /codon_start=1
                     /translation="MTKYNEAYCDVLIVGAGPAGVMAAAHLLSYGTTARPHRVRIFDA
                     TKEVNGSDESTESLSTDVIADALNSGASGPEKDAASTTEDLPMLVTTLQVSDVLHDTG
                     DDTKIAYRETATEQQVLLLADTTANTSSTMNPRSMCEAGCRFHQIYQGHCFPEYELDS
                     ERLRSVDGRAQVLEDEHETGQLRLERLGRPEELLELDEENSMSVVTNLKAAPYKFLMK
                     DVDENFPGELSTSGGKTTSISADESAIDAALHAVWDADDLGAAWHLDEASGLRAVDWN
                     AAQWFKSGQPWTPDAAKSLQEGRVFLAGDARHRHPPLTGIGKNTSIADCYNLTWKLLG
                     VLLGVARADPARTYVAERVYIRMRAATDIAVDAEMESLAAKWITVQLTLSRSWISSAK
                     EAERWDAVLRDSAMSASKPMWTTSDMRASFDAGLMGHGHAHDHVTPTIKEFASSSISR
                     SISELASTSWWESRGWGNGGPFESLMEDARWTGAVESNCRYAAYDRDAPVLHEHVAWV
                     TRFTSRARTAVLEAAVGQAHVVDCWDVGLVEPALDDLDSAGAGLHVAHHADQWPAQLD
                     EAVWPRESLSDWRIVTDTSATGEGYQTSPREAPGDYADLNADNAKAHFNGQFAGHKAY
                     GDAAAADGGGCHGRILVGPAVRGRHLHREIPLGEECQRAAQPLFKEV"
BASE COUNT      461 a    626 c    708 g    396 t
ORIGIN      
        1 aggcctggga gatgtaccgc gatggcaagc gcggtgcttg ctacagtgga gggcaacggc
       61 ggcgcgctgg aggtggcgcg ctatgacgaa gtacaacgag gcgtactgcg acgtactcat
      121 cgttggggcg ggacccgccg gagtaatggc cgccgcgcat ctgctatctt atggaactac
      181 ggcgcggccg caccgtgtac gtatcttcga cgcgacgaag gaagtcaatg gctctgacga
      241 aagtaccgag agtctctcga cagatgttat cgccgacgct ttgaattctg gcgcgagcgg
      301 gccggagaag gacgccgctt ctacaacaga ggatctgccg atgctggtca cgaccctgca
      361 agtgtccgat gttcttcacg acaccgggga cgacaccaag atcgcctatc gcgagacagc
      421 tactgagcaa caagtactcc ttcttgctga caccactgca aacacatctt cgacgatgaa
      481 cccgagaagt atgtgcgaag ctggctgccg gtttcaccag atctatcagg gccattgctt
      541 cccggagtac gagctcgaca gcgagaggct tcgatccgtt gatggccgtg ctcaagtact
      601 agaggatgaa catgagacgg gacaacttcg acttgagagg ctcggaagac cagaagaact
      661 tctggagctg gacgaggaaa acagcatgag cgtcgtgacc aacctgaaag ccgcgcccta
      721 caagtttctg atgaaggatg tggacgagaa ctttcccggc gagctgtcta catcgggtgg
      781 gaagaccact tcaatttctg cggacgagtc tgccatcgac gccgccctac atgccgtttg
      841 ggacgctgat gacctcggtg ctgcctggca tctagacgag gcatccggac ttcgagcggt
      901 cgactggaat gcggcgcagt ggttcaagtc cggtcagccc tggacgcccg atgcggccaa
      961 gtcgctgcaa gagggccgtg tgttcctcgc gggcgacgcc cgtcatcgcc acccgcccct
     1021 caccggcatc ggtaagaaca ccagtatagc cgattgctat aacctcacct ggaagctcct
     1081 cggcgtcctg ctgggcgtgg cgagggcaga ccctgctcga acctacgttg ccgagcgggt
     1141 gtacatccgc atgcgtgcgg ccacggacat tgcggtagat gcagagatgg agtcactcgc
     1201 ggcgaagtgg atcacggtgc agctcaccct ctcgcgctca tggatatcga gcgcgaagga
     1261 ggcagaacgc tgggatgctg tgctccggga ctcagcgatg tctgcatcga agccgatgtg
     1321 gacgacgagc gacatgcgcg cgtcattcga tgcgggactt atgggccacg gtcacgcgca
     1381 tgaccacgtc acgcccacca tcaaggagtt cgcctcaagc tcgatcagcc gatcgatttc
     1441 cgagctggcc agtacgtcat ggtgggaatc ccggggctgg ggcaacggcg ggccttttga
     1501 gtcgctcatg gaggacgcga ggtggaccgg tgcggtggaa tcgaactgca ggtacgccgc
     1561 gtacgaccgc gacgcaccgg tgctgcacga gcacgttgcg tgggtgacac gcttcacgtc
     1621 acgggcccgt acggcggttc ttgaggcggc agtcggccaa gcccatgttg ttgattgctg
     1681 ggacgtcggg ctcgtcgagc ccgcgctcga tgatctcgac tctgctggag cagggctgca
     1741 cgttgcccat cacgctgatc aatggccagc gcagctggac gaagctgtat ggccacgaga
     1801 gagtttgtcg gactggcgga tcgtcaccga cacttcagct acgggtgagg gttatcaaac
     1861 gagcccgagg gaagcgcctg gagactacgc ggaccttaac gcagacaacg ccaaggcgca
     1921 tttcaacggg cagtttgcgg ggcacaaggc gtatggggac gctgccgccg ccgatggtgg
     1981 aggctgccat gggcgcattc ttgtagggcc ggctgttcga ggacgacatc tacaccgaga
     2041 aattcctctc ggcgaggagt gccaacgcgc agcgcagccg ctgttcaagg aggtttgaaa
     2101 ggagacccac gcgatgctga tacccgaccc aaggtggacg tatgcgtgaa cagacccagg
     2161 agtcactgca tagcgcgacc accgaaagct t
//