From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: R.capsulatus genes for potential iron-surfur protein, membrane
Date: 6 Jan 93 07:02:19 GMT
Distribution: bionet
Checksum: 64438 8

LOCUS       RCAFDXC      2977 bp ds-DNA             BCT       06-JAN-1993
DEFINITION  R.capsulatus genes for potential iron-surfur protein, membrane
            protein and FMN-protein, complete cds.
ACCESSION   D13625
KEYWORDS    FMN-protein; FdxC; ORFU1 product; ORFU2 product; ORFU3 product;
            ferredoxin; iron-sulfur protein; membrane protein.
SOURCE      Rhodobacter capsulatus (strain SB1003) DNA.
  ORGANISM  Rhodobacter capsulatus
            Prokaryotae; Bacteria; Gracilicutes; Anoxyphotobacteria; Purple
            nonsulfur bacteria.
REFERENCE   1  (bases 1 to 2977)
  AUTHORS   Saeki,K., Tokuda,K., Fujiwara,T. and Matsubara,H.
  TITLE     Nucleotide sequence and genetic analysis of the region essential
            for functional expression of the gene for ferredoxin I, fdxN, in
            Rhodobacter capsulatus
  JOURNAL   Plant Cell Physiol. (1993) In press
  STANDARD  full automatic
COMMENT     Submitted (10-NOV-1992) to DDBJ by: Kazuhiko Saeki Department of
            Biology Faculty of Science Osaka University 1-1 Machikaneyama
            Toyonaka, Osaka 560 Japan Phone:  06-844-1151 Email:
            ksaeki@niguts.nig.ac.jp Fax:    06-855-8139.
FEATURES             Location/Qualifiers
     CDS             2974..>2977
                     /product="FdxC"
                     /gene="fdxC"
                     /codon_start=1
     promoter        complement(1290..1308)
                     /label=Pu2
                     /note="-24/-12 type promoter (nif-promoter) before ORFU2"
     protein_bind    1505..1520
                     /function="upstream activator for both Pu1 and Pu2
                     promoters"
                     /bound_moiety="NifA"
     promoter        1592..1608
                     /label=Pu1
                     /note="-24/-12 type promoter (nif-promoter) before ORFU1"
     CDS             complement(64..627)
                     /function="required for nitrogen fixation"
                     /gene="ORFU3"
                     /note="183 aa (19 kD) protein with 2[4Fe-4S] culsters"
                     /product="ORFU3 product, potential iron-sulfur protein"
                     /codon_start=1
                     /translation="MIAAAASMSALGLGLGYLLGAAARKFHVETPPIVEEIAKILPGT
                     NCGACGFPGCNGLAEAMAEGNAPVTACTPGGRDVALALAEIVTVEAGADAGPIAEIEP
                     MVAFVFEDHCTGCQKCFKRCPTDAIVGGAKQIHTVVMDACIGCDACIEVCPTEAIVSR
                     VKPKTLKTWYWDKPQPGLVAASAETAA"
     CDS             complement(648..1229)
                     /function="required for nitrogen fixation"
                     /gene="ORFU2"
                     /note="193 aa (20 kD) with 6 membrane spanning region"
                     /product="ORFU2 product, potential membrane protein"
                     /codon_start=1
                     /translation="MQDFLLVLLSTALVNNVVLVKFLGLCPFMGVSRKTDAAIGMGLA
                     TTFVITVASAACWLVEALILEPLDLKFLRILSMILVIAAIVQFIETVMRKVTPDLHKA
                     LGIYLPLITTNCAVLGLPLMYIQGHLSLAMSTLSGFGASVGFTLVLVIFAGMRERLAQ
                     LSVPAAFAGTPIAFVSAGLLGLAFMGFAGLVHV"
     CDS             1651..2958
                     /gene="ORFU1"
                     /partial
                     /note="435 aa (48 kD) protein with flavodoxin-like domain"
                     /product="ORFU1 product, potential FMN-protein"
                     /codon_start=1
                     /translation="LSVPPFTIRPAAPRLDGPTGPVAVAPGVHWVGALDPGLRNFDVI
                     LKTANGTTYNAYAVRGSEGVAVIDTVKAEFAGDFFARLEAVARYDEIRLIVLNHLEPD
                     HTGAVPELLRRAPQAQVRLSPRGLPMLRALLKDDFERYDIKGVTTGQSVSLGDRDLQF
                     FTTPFVHWPDTQCTWLAAERVLFTCDLFGSHYCDGRLFNDLVGDFRFSFEYYFDRIMR
                     PFRSFVAQALDLIEPLDFGIIAPAHGPILRSHPRDYLTHTRRLISSRLAAETGSEKTL
                     LIFYVSAYGATAQLAQAIHDGAAESPDVRVSLFDLEGGEITPFLDLIEEADGIALGTP
                     TINGDAVRTIWEMLAALVDIETRGKLGAAFGSYGWSGEAVRLVETRLQGLKMRLPEPG
                     LRVKLHPSAAELKEGRAFGRRLADHLTGRAAPREVDFAEIAAR"
BASE COUNT      484 a    974 c   1005 g    514 t
ORIGIN      
        1 ggatcccgcc gcggatcgaa aaggattgca gcggatgaaa cagggtggcg atcgagggaa
       61 gtctcatgct gcggtctccg cgcttgccgc gaccagaccc ggttgcggct tgtcccaata
      121 ccaggtcttg agggtcttcg gcttgacgcg gctgacgatc gcctcggtcg ggcagacctc
      181 gatgcaggcg tcgcagccga tgcaggcgtc catcaccacg gtatggatct gcttggcgcc
      241 gccgacgatc gcatcggtcg ggcagcgctt gaaacatttc tggcagccgg tgcagtgatc
      301 ctcgaaaaca aaggcgacca tcggctcgat ctcggcgatc gggcctgcgt cggcgcccgc
      361 ctcgacggtg acgatctcgg ccagggccag cgccacatcg cgcccgcccg gggtgcaggc
      421 ggtcaccggc gcgttgccct cggccatcgc ctcggccaga ccgttgcagc cgggaaagcc
      481 gcaggcgccg caattggtgc cgggcaggat cttggcgatt tcctcgacga tcggcggggt
      541 ctcgacgtgg aacttgcggg cggcggcgcc gagcaggtag cccagcccga gcccgagggc
      601 cgacatggaa gcggcggctg cgatcatggc aggtctcctt tcgacgatca gacgtgcacg
      661 agcccggcaa agcccatgaa ggcgaggccc agaagccctg cggacacgaa ggcgatgggg
      721 gttccggcaa aggccgcggg caccgaaagc tgcgccagcc gttcgcgcat gcccgcaaag
      781 atcaccagaa cgagcgtgaa accgaccgag gcgccaaagc cgctcagcgt gctcatcgca
      841 aggctcagat ggccctgaat gtacatcagc ggcaggccga ggacggcgca gttcgtggtg
      901 atcagcggca gatagatgcc gagcgccttg tgcaggtcgg gggtgacctt gcgcatcacc
      961 gtctcgatga actgcacgat cgcggcgatg accaggatca tgctcaggat gcgcaggaat
     1021 ttcaggtcga gcggttccag gatcagcgcc tcgaccagcc agcaggcggc cgaggcgacg
     1081 gtgatcacga aggtcgtggc cagccccatg ccgatcgcgg catcggtctt gcgcgagacg
     1141 cccatgaacg ggcagagccc gaggaatttc accagcacga cgttgttcac gagcgcggtg
     1201 ctcaagagga cgagaaggaa gtcttgcatc ggtctccccg gttggctgtg ccgtcgcgcc
     1261 aaggcgtggc gaagggcttc atcggggatc aagcaagggc tgtgccaagc gggcggatcg
     1321 cggcctgtcg cgccccggcg tgcccgcccg cacgcgtgtc cgcgcgcgca tccgcccccg
     1381 cgcccgcgat aaagatgtga gcgctcccgc cgcagcggca aagcccgtcg gtttcggggg
     1441 gaaatcgccc cgatcacggc ttcgggagcc ggttttccgc gtgttttcgg cgctttcgcc
     1501 gcattgtcgg gtttccgaca atttgtcggg gccgcgccgc ggcttctgtc gtcttgcggc
     1561 cccgcacccc tgtttccttg ggcttccgcg cttggcacgg ttcttgctgc acatctgatg
     1621 cggcctgccc gcaaccacag gagtgttccc ttgtccgttc cgcctttcac catccgcccg
     1681 gccgcgcccc gccttgacgg ccccaccggc cctgtcgcgg tggcgcccgg cgtgcattgg
     1741 gtgggggcgc tcgatccggg gctgcgcaat ttcgacgtga tcctgaaaac cgcgaatggc
     1801 accacctaca atgcctatgc cgtgcgcggg tcagaggggg tcgcggtgat cgacacggtc
     1861 aaggccgaat tcgcgggcga cttctttgcc cggctggaag ccgttgcccg ctatgacgaa
     1921 atccgcctga tcgtgctgaa ccatctggag cccgatcaca ccggcgccgt gcccgaactc
     1981 ttgcgccgcg caccgcaggc gcaggtccgg ctgtcgcccc gcggcctgcc gatgctgcgc
     2041 gcgcttttga aggacgactt cgagcgttac gacatcaagg gcgtcaccac cgggcaatcg
     2101 gtcagcctgg gcgaccgcga tctgcagttc ttcaccacgc ctttcgtgca ttggcccgac
     2161 acgcaatgca cctggctggc ggcggaacgg gtgctgttca cctgtgacct gttcggcagc
     2221 cattattgcg acgggcggct gttcaatgac cttgtcggcg atttccggtt ttcttttgag
     2281 tattacttcg accggatcat gcgccccttc cgcagtttcg tggcgcaggc gctcgacctg
     2341 atcgagccgc tcgatttcgg catcatcgcc cccgcgcatg ggccgatcct gcgcagccat
     2401 ccgcgcgatt atctgaccca tacgcggcgg ctgatttcct cgcggctcgc cgccgaaacc
     2461 ggcagcgaaa agacgctgct gatcttttac gtctcggcct atggcgccac cgcgcagctg
     2521 gcgcaggcga tccatgacgg tgccgccgaa agccccgatg tgcgggtgtc gctctttgat
     2581 ctggagggcg gcgagatcac ccccttcctg gatctgatcg aggaggccga cggcattgcg
     2641 ctgggcacgc cgacgatcaa cggcgatgcg gtgcgcacga tctgggaaat gctggcggcg
     2701 ctggtcgata tcgaaacccg cggcaagctg ggggcggctt tcggctccta tggctggtcg
     2761 ggcgaggcgg tgcggctggt cgaaacccgg ctgcaggggc tgaagatgcg tctgccggaa
     2821 ccggggttgc gggtgaagct gcacccctcc gcggcggaac tgaaagaggg gcgggccttc
     2881 gggcgccgcc ttgccgatca cctgaccggc cgggccgcgc cccgcgaggt cgacttcgcc
     2941 gaaatcgcgg cgcgctgaaa caaaggacgg accatgg
//