From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Clostridium longisporum endo-1,4-alpha-D-glucanase (celA) gene,
Date: 29 Sep 92 10:02:52 GMT
Distribution: bionet
Checksum: 62779     7

LOCUS       CLOEGAA      3620 bp ds-DNA             BCT       29-SEP-1992
DEFINITION  Clostridium longisporum endo-1,4-alpha-D-glucanase (celA) gene,
            complete cds.
ACCESSION   L02868
KEYWORDS    cellulase; cellulose-binding domain; endo-1,4-alpha-D-glucanase.
SOURCE      Clostridium longisporum DNA.
  ORGANISM  Clostridium longisporum
            Prokaryotae; Firmicutes; Endospore-forming rods and cocci;
            Bacillaceae.
REFERENCE   1  (bases 1 to 3620)
  AUTHORS   Mittendorf,V. and Thomson,J.A.
  JOURNAL   Unpublished (1992)
  STANDARD  full staff_entry
FEATURES             Location/Qualifiers
     -35_signal      1167..1170
                     /gene="celA"
                     /note="putative"
     -10_signal      1191..1195
                     /gene="celA"
                     /note="putative"
     RBS             1230..1235
                     /gene="celA"
                     /note="putative"
     CDS             1246..2799
                     /standard_name="EGA"
                     /product="endo-1,4-alpha-D-glucanase"
                     /gene="celA"
                     /evidence=EXPERIMENTAL
                     /codon_start=1
                     /translation="MKRSLLKTCSIIAGATIIFSSLSISRNPLEVQAASMRSASEIVQ
                     EMGVGWNLGNTLDAKITNLSYNTSPISFETGWGNPVTTKAMIDKIKNAGFKTIRIPTT
                     WGEHLDGNNKLNEEWVKRVKEVVDYCIADDLYVILNTHHEGNWVIPTYAKESSVTPKL
                     KTLWTQISEAFKDYDDHLIFETLNEPRLEGTPYEWTGGTSESRDVVNKYNAAALESIR
                     KTGGNNLSRAVMMPTYAASGSSTTMNDFKVPDDKNVIASVHAYSPYFFAMDTSSNSVN
                     TWGSSYDKYSLDVELDSYLNTFKSKGVPVVIGEFGSINKNNTSSRAELAEYYVTAAQK
                     RGIPCVWWDNNYAETNKGETFGLLNRSTLNWYFSDIKDALIRGYKNVHPEATEDDKPS
                     TDVTNPDSGNTKPDSGNTNPGTETTTPTDNEKISITSKINDWGGAYQADFTLKNNTSS
                     DINNWSFKIKKNDIVFTNYWDVKITEENGYYVVTPQAWKTTILANSSIVISIQGTGKV
                     ISNFEYKFD"
     sig_peptide     1246..1350
                     /gene="celA"
                     /evidence=EXPERIMENTAL
                     /codon_start=1
BASE COUNT     1286 a    579 c    483 g   1272 t
ORIGIN
        1 tactaacaca tatagaaagc atttcagaat tcttcttaaa ccacaatctt caaatctttt
       61 caaaaatacc attatcatca gggtttacca tgtctacact tccagtaatg atttatatat
      121 gtataataaa tactggtata ggttatgcat gctattttaa ggctctagaa aaaacttcag
      181 caactaagac atctttagta tttttcttca aaccaatttt agctccaata ctagctttaa
      241 taatcttaaa agaatcttta tcttttaata tgcttatagg tatattatta atattgtgtg
      301 gttcaatgac atcaatactt ccatcactta aaaataataa aaatattaag cttaaaaagg
      361 cagcaataaa ataaaagggt atgtaatttt gaacaaaata gactgttcaa aactacatgc
      421 cctttaaaat atatagcttt ctagcttaag cacctttcca aaatctgata caatatcaaa
      481 gctcatcttt tttccattaa gcttttttat taactgtaaa cttggtatat ttaaaatatc
      541 agctgcaaaa ataagtcctt ctatcttata attttctttt aaaaaatctt ttataagaag
      601 taaagcttca taagcatatc ccttttttct ttcatcttca catatccaaa ttccaacttc
      661 cggacattca tccttcaaat tataaagctc tatacttcca acaaacttat cttcttcatt
      721 ccacaagcta agcatagtat tatcaccctt tatacttaaa tttataaatt cctgcaaaaa
      781 actttcagct tcttttatag acttaaaaga atttgcaagc tggtatttta ctacctcttc
      841 agtaaagttt tcataatata aagctgcctc ttcaattttt gtagaagtta ttctaagcct
      901 ttttcctttt atttcaattg tattattcat aaaaaacctt cttaaatatt cttaatgtat
      961 tctttaactg atataacctt ttaatctgaa attcttcata gcaatacaaa tcaactatac
     1021 tgttcattat agaataaata taaaaattat aaaagaaaaa tttatgttat tttacatgct
     1081 ggtaatgcta aaactggtag tattctaatt tttattaata acaatgacta actttatgta
     1141 ttaatagcac actttcacga attattattg aatttatttc tttaagatat tatattgaat
     1201 ttatgtaaat aattacatca aagaaataag ggagatgatt aaactatgaa aagatcttta
     1261 ttaaaaactt gttctatcat tgctggagca acaattattt tctcatcact ttcaatttca
     1321 agaaatcctc ttgaagtaca agctgcttct atgagatctg caagtgaaat tgtgcaggaa
     1381 atgggagttg ggtggaatct tggaaatact ttagatgcta agattactaa cctgtcttat
     1441 aatacttcac caatatcctt tgaaacaggc tggggaaatc ctgttactac aaaggctatg
     1501 attgataaaa tcaaaaatgc tggatttaaa accataagaa taccaactac ttggggagaa
     1561 cacttagatg gtaataacaa acttaatgaa gaatgggtaa agagagttaa agaagttgtt
     1621 gattattgta tagcagatga tctttatgtt atcttaaata ctcatcatga aggaaactgg
     1681 gttattccaa cttacgctaa agaatcttca gtaactccaa aactaaaaac tctttggact
     1741 caaatatctg aagctttcaa agattatgat gatcatttaa tatttgaaac tcttaacgaa
     1801 ccaagacttg aaggaactcc ttatgaatgg acaggtggta caagtgaatc tcgtgatgtt
     1861 gtaaataaat ataacgcagc tgctttagaa tctataagaa aaactggtgg taataactta
     1921 tctagagctg ttatgatgcc aacttatgca gcttctggtt catctactac aatgaatgat
     1981 tttaaagttc cagatgataa aaatgtaatt gcatctgttc atgcatattc tccttacttc
     2041 tttgctatgg acacaagcag caattcagtt aacacatggg gaagttctta tgacaaatat
     2101 tctttagatg tagaattaga ttcttactta aatactttta aatctaaagg agttcctgtt
     2161 gttattggtg aatttggttc tataaataaa aataatactt cttcaagagc agagcttgct
     2221 gaatattatg ttacagctgc tcaaaagaga ggtattcctt gtgtatggtg ggataacaac
     2281 tatgctgaaa ctaataaggg tgaaactttt ggattactaa atagaagtac tttaaactgg
     2341 tactttagtg atattaaaga tgctttaatt agaggatata aaaatgtaca tcctgaagct
     2401 actgaagatg ataaaccaag cacagatgta accaaccctg attcaggcaa tactaaacca
     2461 gacagtggta atactaatcc tggtacagaa actactactc caacagataa tgaaaagatt
     2521 tcaataactt caaaaattaa tgattggggc ggtgcttacc aagctgattt cactttaaaa
     2581 aataacactt caagtgacat taacaattgg tcttttaaga taaagaaaaa tgatattgtg
     2641 tttactaatt actgggatgt taagataact gaagaaaatg gatattatgt tgttacacct
     2701 caagcttgga aaacaactat cctcgcaaat tcttctatag ttatttctat tcaaggcact
     2761 ggaaaagtaa ttagtaattt tgagtataaa tttgactaga taacctaaat aaaatttaag
     2821 cctaaacaat ataacttcta tcttgtttag gcttaaatta tactactcgt ccatcatatt
     2881 acggttatta tatgagtaca cattataatc tctccaaata agttcaagat ttgtaaatgt
     2941 atctgaaata ctttcctttt ctctcattcc tactgctatt ataagtgcat ttattactga
     3001 taaaggtgct actaatgaat caacaaatga agccatatta ctttgagcta ttaacgtata
     3061 atctgcatgt gaagcaagtg gagataataa actatctgta agtgctaaaa ccttagctcc
     3121 tcttctctta gaaaaatcta aggcatctat agtttttgtt gcatatcttg ggaaaccaat
     3181 ccctataact aaatctcctt ctcccacatt aatcatttgt tcaaaaatgt ctgaaattcc
     3241 gtagcttact attcttacat tttgaagaat tatattcaaa taaaatccta agaattcagc
     3301 aagagctgta gaacttctaa ggcctattat atatattttc tttgcttcaa aaattgaatt
     3361 taaaacttgt tcaaaggtat aagggtttat cttttcaaga gttgctctta tattttccat
     3421 atctgccttt aatactccct ttaaagtatc tccatcagaa ataaggtcct ttgaaagctc
     3481 taatctttga acagttgtta gcttattttt tattagctct tgaagagctt tttgtaattt
     3541 aggataacct aaaaatccta attcatttgc aaatcttact actgtagatt cagatacacc
     3601 tacagaatct ccaagctttg
//