From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Dichelobacter nodosus extracellular subtilisin-like protease gene,
Date: 3 Jan 93 22:12:43 GMT
Distribution: bionet
Checksum: 15184 5

LOCUS       DIHEXCSLP    2122 bp ds-DNA             BCT       23-DEC-1992
DEFINITION  Dichelobacter nodosus extracellular subtilisin-like protease gene,
            complete cds.
ACCESSION   L08175
KEYWORDS    extracellular protease; subtilisin-like protease.
SOURCE      Dichelobacter nodosus (library: EGERTON Collection VCS1001) DNA.
  ORGANISM  Dichelobacter nodosus
            Prokaryotae; Gracilicutes; Scotobacteria; Anaerobic gram-negative
            straight, curved and helical rods; Cardiobacteriaceae.
REFERENCE   1  (bases 1 to 2122)
  AUTHORS   Good,R.T., Brandon,R.B., Langford,C.J. and Moses,E.K.
  TITLE     The cloning, nucleotide sequence and expression of a structural
            gene encoding a member of a family of extracellular proteases from
            Dichelobacter nodosus
  JOURNAL   Unpublished (1993)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     terminator      one-of(1963..1988,2002..2023)
     RBS             78..83
     CDS             89..1876
                     /product="subtilisin-like protease"
                     /codon_start=1
                     /translation="MKQSGINGVKTLTLVVCAALASQAYAAVNYESANYIGSQPEGSV
                     RFIIKYKDKSQSQQMMTNRSTTSVMNNNNITIAGFNAQFVRTMTIGAGIFAVPDLKTT
                     KEAHLVMDTIASNPDVEYVEVDRWLRPFAAPNDPFYNDQWHYYSEYGVKADKVWDRGI
                     TGKGVTVAVVDTGIVNHPDLNANVIPGSGYDFIQEAEIAQDGDGRDSNPADAGDWHSN
                     WACGKYPDPRYEKRNSSWHGSHVAGTIAAVTNNRIGVSGVAYDAKIVPVRVLGRCGGY
                     NSDINEGMYWAAGGHIDGVPDNKHPAQVINMSLGGPGVCGSTEQTLINRATQLGATII
                     VAAGNDNIDAYGVTPASCDNILTVGATTSNGTRAYFSNHGSVVDISAPGAGITSTVDS
                     GARYPSGPSYSLMDGTSMATPHVAGVAALVISAANSVNKEMTPAQVRDVLVRTVSSFN
                     GTPDRRIGAGIVDADAAVNAVLDGNVVERPIDELKPQAEYRNPQIKLIRDYQMMFSEI
                     KVNGRPGNTKFAVVKADIRHTDPSQLKLRLVSPKGYEYAVHYDNIKNKSSELITFPRD
                     EQMNGYWRLKIVDTKRGVTGYTRGWSVAF"
BASE COUNT      593 a    449 c    479 g    601 t
ORIGIN      
        1 gatcttggca catgtgaaaa gaagttaatc tctagttaaa tcccgttttc tccaccccac
       61 gtagtcaggt tatttttagg tgaaactcat gaaacaatcc ggaatcaatg gcgttaaaac
      121 gctgaccctt gtcgtatgcg ccgcgcttgc aagccaagct tatgccgccg taaattacga
      181 atccgctaat tatattggca gccaaccaga aggcagcgtt cgctttatca ttaaatacaa
      241 agataaaagc caatcccaac aaatgatgac caaccgcagc acaaccagcg ttatgaataa
      301 taacaacatt acaattgctg gatttaatgc tcaattcgtg cgcacaatga caattggcgc
      361 gggcattttt gctgtacccg atttaaaaac aacgaaagaa gcgcatttgg taatggatac
      421 catcgcttcc aatcctgatg ttgaatacgt tgaagttgac cgttggcttc gtccttttgc
      481 cgctccaaat gatccgttct ataacgatca atggcactat tacagcgaat atggcgttaa
      541 agccgataaa gtttgggatc gtggcatcac aggaaaaggc gtaactgttg ccgtagtaga
      601 tacaggtatc gtcaatcacc ctgatttaaa tgcaaacgta attcccggat ctggttatga
      661 ctttattcaa gaagcggaaa ttgctcaaga tggcgatggt cgtgattcta atcctgctga
      721 cgccggcgat tggcacagta actgggcgtg cggtaagtat cccgatccgc gttatgaaaa
      781 aagaaacagt tcttggcacg gctctcacgt tgctggaacc attgcagccg taactaataa
      841 ccgcattggt gtttctggcg ttgcctacga tgcgaaaatt gttccggtgc gtgtattagg
      901 tcgttgcggt ggttataact ctgatattaa cgaaggtatg tattgggcag cgggcggtca
      961 tattgatggc gtgcctgata acaaacaccc agcccaagtt attaacatga gtttgggcgg
     1021 acctggtgtt tgcggttcta ccgaacaaac tttaattaac agagctacac aattgggcgc
     1081 aaccattatc gttgcagcag gtaacgataa tatcgatgca tacggagtaa ccccagcaag
     1141 ctgcgacaat attttaacag tgggtgcaac cacttctaac ggtactcgcg cctatttctc
     1201 taaccacgga agcgttgttg atatttctgc accgggcgcc ggcattactt ctaccgttga
     1261 ctctggagca cgttatccaa gtggtccgag ttatagtttg atggacggta cttctatggc
     1321 gacaccgcac gttgctggcg ttgctgcatt agtaatttct gccgctaata gcgtcaataa
     1381 agaaatgaca ccggcacaag ttcgcgatgt tttagtgcgc acagttagca gctttaacgg
     1441 tactccagat cgcagaatcg gcgcaggtat tgttgatgct gatgctgctg ttaatgccgt
     1501 tttagatggt aatgttgttg aacgtccgat cgacgaatta aaaccgcaag cggaataccg
     1561 caatcctcaa attaaattaa ttcgcgatta tcaaatgatg ttcagcgaaa ttaaggttaa
     1621 cggtcgtcca ggaaatacta aatttgcggt tgtaaaagca gatattcgtc atactgatcc
     1681 gagtcaatta aaactgcgtc tcgtttctcc gaaaggttat gaatatgctg ttcattatga
     1741 caacattaaa aataaatcct ccgagctcat cactttccca agagatgaac aaatgaatgg
     1801 ttattggaga ctgaaaatcg tggataccaa acgcggtgtt accggttata ccagaggttg
     1861 gagtgttgca ttctaacttt attgagttgt cataattcaa ttaaagcctc ccgttttggg
     1921 acggtttctt gttttttatt ttaggagact tgtcatgcgt gaaaaaattg cggcaccgct
     1981 caatttttaa cttctattcg ccaatttttt gctcaaaaaa atgtgctcga agtggatacg
     2041 ctttattatc accagcggga gtgacggata ttaatttgcg atcattggcg gtgaagattt
     2101 aggttattta tcacttcacc ag
//