From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: T.brucei gene encoding trypanothione reductase.
Date: 25 Jul 92 10:07:14 GMT
Distribution: bionet
Checksum: 59446     5

LOCUS       TRBTRG       1522 bp ds-DNA             INV       25-JUL-1992
DEFINITION  T.brucei gene encoding trypanothione reductase.
ACCESSION   X63188
KEYWORDS    trypanothione reductase.
SOURCE      Trypanosoma brucei DNA.
  ORGANISM  Trypanosoma brucei
            Eukaryota; Animalia; Protozoa; Sarcomastigophora; Mastigophora;
            Kinetoplastida; Trypanosomatina; Trypanosomatidae.
REFERENCE   1  (bases 1 to 1522)
  AUTHORS   Fairlamb,A.H.
  JOURNAL   Unpublished (1991)
  STANDARD  full staff_review
REFERENCE   2  (bases 1 to 1522)
  AUTHORS   Aboagye-Kwarteng,T., Smith,K. and Fairlamb,A.H.
  TITLE     Molecular characterization of the trypanothione reductase gene from
            Crithidia fasciculata and Trypanosoma brucei: comparison with other
            flavoprotein disulphide oxidoreductases with respect to ...
  JOURNAL   Mol. Microbiol. (1992) In press
  STANDARD  full staff_review
COMMENT     *source: clone_library=lambda gt11; *source: clone=TRd3; *source:
            isolate=ILTat 1.1;
            From EMBL    entry TBTRG;  dated 17-JUN-1992.
FEATURES             Location/Qualifiers
     CDS             19..1497
                     /function="Reduction oftrypanothione disulphide in the
                     cytosol"
                     /EC_number="1.6.4.8"
                     /standard_name="TR"
                     /note="Redox active cysteines52 & 58. Active site base:
                     His461. Homodimer.Similarity to glutathione reductases and
                     lipoamidedehydrogenases. Enzyme-bound FAD.
                     Location:cytosol."
                     /product="trypanothione reductase"
                     /codon_start=1
                     /translation="MSKIFDLVVIGAGSGGLEAGWNAATLYKKRVAVIDVQTHHGPPH
                     YAALGGTCVNVGCVPKKLMVTGAQYMDHLRESAGFGWEFDGSSVKANWKKLIAAKNEA
                     VLDINKSYEGMFNDTEGLDFFLGWGSLESKNVVVVRETADPKSAVKERLQADHILLAT
                     GSWPQMPAIPGVEHCISSNEAFYLPEPPRRVLTVGGGFISVEFAGIFNAYKPPGGKVT
                     LCYRNNLILRGFDETIREEVTKQLTANGIEIMTNENPAKVSLNTDGSKHVTFESGKTL
                     DVDVVMMAIGRIPRTNDLQLGNVGVKLTPKGGVQVDEFSRTNVPNIYAIGDITDRLML
                     TPVAINEGAALVDTVFGNKPRKTDHTRVASAVFSIPPIGTCGLIEEVAAKEFEKVAVY
                     MSSFTPLMHNISGSKYKKFVAKIVTNHSDGTVLGVHLLGDGAPEIIQAVGVCLRLNAK
                     ISDFYNTIGVHPTSAEELCSMRTPSYYYLKGEKMETLPESSL"
                     /citation=[1]
                     /citation=[2]
BASE COUNT      381 a    361 c    430 g    350 t
ORIGIN
        1 tttttgaatc ttaagatcat gagcaaaatc tttgatttgg ttgttatcgg cgcagggagt
       61 ggcgggctcg aggccgggtg gaatgccgca acattgtaca aaaagcgcgt tgcggtgatc
      121 gatgtgcaaa cgcatcacgg gcctccccac tatgcagcct tgggcgggac gtgtgtgaac
      181 gtcgggtgcg tccccaaaaa gttgatggtc acaggtgcac agtacatgga ccaccttcgc
      241 gagtctgcgg ggtttggctg ggaatttgac ggctcgtccg ttaaggcaaa ctggaaaaag
      301 ctgatcgcgg caaagaacga agccgtactg gatatcaaca agagttatga aggaatgttc
      361 aacgacaccg aaggcctaga tttttttttg ggttgggggt cgttggagtc gaagaatgtt
      421 gttgttgtgc gtgagacggc tgatcccaag agcgccgtga aggagcgcct gcaggcagat
      481 catatactcc ttgccactgg gtcgtggcct cagatgcctg cgatccctgg cgttgagcat
      541 tgtataagca gtaacgaagc gttctacctt ccggagcccc cccgccgcgt gttgactgtg
      601 ggtggtggct ttatatccgt ggagtttgcc ggtatattta atgcctacaa gccaccaggg
      661 ggcaaggtaa cactctgcta ccgtaacaat ctcattcttc gtggatttga cgagacaatc
      721 cgggaggagg taacgaaaca actcaccgcc aatggtattg agataatgac aaatgaaaac
      781 cctgctaaag tgagtctcaa caccgatgga agcaaacatg tgacgttcga gagtggaaag
      841 actctggatg ttgacgtggt tatgatggca atcggtcgca ttccccgcac caacgacctc
      901 cagttgggga acgtcggtgt aaaacttact cccaagggag gtgtgcaggt ggatgagttc
      961 tcccgcacta acgtgccgaa tatttacgcc atcggcgaca tcacagaccg cctgatgctc
     1021 actcctgtgg ccatcaacga aggtgcagcc cttgtggata cagtcttcgg taataaacca
     1081 cggaaaacag accacacacg tgttgctagt gccgtctttt cgatacctcc cattggaacc
     1141 tgtggcctca tcgaggaagt tgcggcgaag gaattcgaaa aggttgcggt ttacatgtct
     1201 agcttcactc cgctcatgca caacatcagc gggtcgaagt acaaaaagtt tgtggcaaag
     1261 attgtcacaa accacagtga tggcacagtg cttggggtac atcttcttgg tgacggtgct
     1321 ccagagataa ttcaggccgt cggtgtgtgc ctaaggctga acgcaaagat cagcgacttt
     1381 tacaacacca taggtgtcca ccccacaagt gcggaagagc tgtgcagtat gcgcacccct
     1441 agttattact acctaaaagg ggaaaagatg gagactctgc ccgaatcgtc cttgtaaggg
     1501 gcgtgaagga gctcgcgtac ac
//