From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: B.stearothermophilus insertion sequence IS5377
Date: 4 Sep 92 10:02:31 GMT
Distribution: bionet
Checksum: 58155     4

LOCUS       BACIS5377    1249 bp ds-DNA             BCT       04-SEP-1992
DEFINITION  B.stearothermophilus insertion sequence IS5377
ACCESSION   X67862
KEYWORDS    transposable element.
SOURCE      Bacillus stearothermophilus DNA.
  ORGANISM  Bacillus stearothermophilus
            Prokaryotae; Firmicutes; Endospore-forming rods and cocci;
            Bacillaceae.
REFERENCE   1  (bases 1 to 1249)
  AUTHORS   Xu,K.Y., He,Z.Q., Mao,Y.M., Shen,R.Q. and Sheng,Z.J.
  TITLE     On two transposable elements from bacillus stearothermophilus
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
COMMENT     *source: strain=CU21;
            From EMBL    entry BSIS5377;  dated 13-AUG-1992.
FEATURES             Location/Qualifiers
     repeat_region   1..20
                     /rpt_type=inverted
     RBS             90..96
     CDS             104..1237
                     /note="putative"
                     /product="transposase"
                     /codon_start=1
                     /translation="MNKHTTLPNLMQKLVSDEEIQLIAEAVGYRDSSRTFTLRELIHF
                     FLLAAMHQWKSFRHGADVGPLYGLPRFHYSTVSKKAKEVPYDIMKRLLALIISKCNRQ
                     TRRSLRFPKPLRVVDSTTVTVGKNRLPWAPYHGERAGVKLHVAYSPEFSLPADVVETT
                     GLRHDGPVGEQLTNAQQVLVEDRAYFKIERLDRFVEQHQLFVIRMKDNIELHQKKSLN
                     RLSSTSSSVQTDFTCQLGTKQCRSTKRHRVVIFRDANGRDIRVVTNLFHASAETIADM
                     YQQRWAVEVFFRWVKQYLNVPTLFGTTENAVYNQLFAAFIAYVLLRWLYDQTKKQTNV
                     SLSFISFVRRFFSGQLPLDWKSGMAAALFEYAQIYGRRMYNFG"
     repeat_region   1229..1249
                     /rpt_type=inverted
BASE COUNT      307 a    317 c    315 g    310 t
ORIGIN
        1 caggcgtgtt gattatccaa taaaatccaa agggtataga aaaaagagca cctttcctgt
       61 agaatgtgag tagcaacaca aacaaaccca ggaggtgctc tctatgaaca agcataccac
      121 actcccgaat ttgatgcaaa aacttgtttc ggatgaagag attcaactga ttgccgaagc
      181 ggttgggtat cgtgattcgt ctcgaacctt tacgttgcgc gagttgattc acttcttcct
      241 gctggccgcc atgcatcaat ggaaaagctt tcgccacgga gccgatgtgg ggcctctgta
      301 tggattgccg cgattccatt attccacagt atccaagaaa gcgaaagaag ttccctatga
      361 catcatgaaa cgcttgttgg cgttgatcat ttccaagtgc aaccgccaaa cccgccgttc
      421 gcttcggttt cccaaaccgc ttcgggtggt ggattcgacg accgtcacgg tcgggaaaaa
      481 ccgcctccca tgggcgccgt atcacggcga acgcgccgga gtgaagctgc acgtcgcgta
      541 ttcgccggaa ttctcgttgc cggcagacgt ggtggaaacg accggactgc gtcacgatgg
      601 cccagtggga gaacagttga cgaacgctca acaagtgctc gtggaagacc gggcgtattt
      661 caaaatcgaa cgcctcgatc gatttgtgga gcagcatcag ctctttgtca ttcggatgaa
      721 ggacaacatc gaacttcatc agaaaaaaag cttgaaccgc ctttccagca catcctcatc
      781 ggttcaaacc gacttcacgt gccagttggg aacgaaacaa tgccgctcca ccaagcgtca
      841 ccgggtggtg atctttcgag atgcgaatgg ccgcgacatt cgggtcgtga cgaacctctt
      901 ccatgcgtct gcggaaacca ttgccgacat gtaccaacaa cgttgggctg ttgaggtctt
      961 tttccgttgg gtgaagcaat atctgaatgt cccgaccttg tttggcacga cggaaaatgc
     1021 ggtatacaac caactgtttg cggcgttcat cgcgtatgtg ttgctgcgat ggctgtatga
     1081 tcaaaccaaa aaacagacga acgtctctct ttccttcatt tcgttcgttc gccgtttttt
     1141 ctctgggcag cttcctctcg attggaaatc cgggatggcc gctgctttgt ttgagtatgc
     1201 ccaaatttat ggaaggcgta tgtataattt tggataatca acactcgtg
//