From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Schizosaccharomyces pombe ferric reductase (frp1+) gene, complete
Date: 29 Dec 92 20:59:27 GMT
Distribution: bionet
Checksum: 54658 6

LOCUS       YSPFRP1A     2337 bp ds-DNA             PLN       11-DEC-1992
DEFINITION  Schizosaccharomyces pombe ferric reductase (frp1+) gene, complete
            cds.
ACCESSION   L07749
KEYWORDS    ferric reductase.
SOURCE      Schizosaccharomyces pombe DNA.
  ORGANISM  Schizosaccharomyces pombe
            Eukaryota; Fungi; Eumycota; Hemiascomycetes; Endomycetales;
            Saccharomycetaceae.
REFERENCE   1  (bases 1 to 2337)
  AUTHORS   Roman,D.G., Dancis,A., Anderson,G.J. and Klausner,R.D.
  TITLE     The fission yeast ferric reductase gene frp1+ is required for
            ferric iron uptake and is homologous to the gp91-phox subunit of
            the human NADPH phagocyte oxidoreductase
  JOURNAL   Unpublished (1993)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     promoter        1..138
                     /gene="frp1+"
                     /note="'confers iron regulated activity to heterologous
                     coding region'; putative"
     enhancer        49..96
                     /gene="frp1+"
                     /note="'required for promoter activity and regulation by
                     iron'; putative"
     mRNA            139..2337
                     /gene="frp1+"
                     /note="'5'end mapped by primer extension, 3'end estimated
                     from mRNA size'; putative"
     CDS             377..2071
                     /gene="frp1+"
                     /note="'required for ferric reductase activity'; putative"
                     /product="ferric reductase"
                     /codon_start=1
                     /translation="MAINSSDKWTVIAICLILGILLAFILMFWLERFRVIIKSNAHKH
                     DPSDKRQIWLEKYYLFVRQIYTYLVTHKVILTLIAVPVVFAISIPFIGMQTPASSHGK
                     QTTQVSTGNWSKNAVAARLGFLACGLYVTSYFFSIKNNPFALLLISSHEKMNYVHRRL
                     SQYAIMIGAIHGFAYIGLAAQGKRALLTARVTIIGYVILGLMVIMIVSSLPFFRRRFY
                     EWFFVLHHMCSIGFLITIWLHHRRCVVYMKVCVAVYVFDRGCRMLRSFLNRSKFDVVL
                     VEDDLIYMKGPRPKKSFFGLPWGAGNHMYINIPSLSYWQIHPFTIASVPSDDFIELFV
                     AVRAGFTKRLAKKVSSKSLSDVSDINISDEKIEKNGDVGIEVMERHSLSQEDLVFESS
                     AAKVSVLMDGPYGPVSNPYKDYSYLFLFAGGVGVSYILPIILDTIKKQSRTVHITFVW
                     SARSSALLNIVHKSLCEAVRYTEMNINIFCHLTNSYPVEEVSSLNSQSARNYSLQYLN
                     GRPDVNDYFKDFLHATGTQTAALASCGSDKLLRHLKSCVNTHSPSTVDLYQHYEEI"
BASE COUNT      712 a    426 c    430 g    769 t
ORIGIN      
        1 gtttgtccaa aatgttgcaa caacagaggt gcaaatttgg ttgatctgat aacgcatcaa
       61 gataatcaga tatgacacaa atagcaaagt ggacaatcga atgcgatatt aataaggctg
      121 cgcttttaac aacattttca acgaatttca actaagaaac aaatagacat acgaaagtag
      181 ggaaactcgg attttgggga acgagtacta atagtttctg aaaattcagt ttaatttttt
      241 gctaactgca aacccaaagg attggatatc tcattccggt ccacttacat tacacccttt
      301 tctaattatt attaataatt tttaatatat acaattttga acaagaagaa tctccataca
      361 tttatacatt tgaaggatgg cgataaattc gagcgacaaa tggaccgtca ttgcaatatg
      421 cttaattctt gggatattac ttgcatttat attgatgttt tggcttgagc gttttcgggt
      481 cataataaaa tcaaatgcac acaaacatga cccatcagat aagaggcaaa tatggctcga
      541 gaaatattat ctttttgttc gacagattta tacgtatctt gtcactcaca aagtaatatt
      601 gactttaatt gctgttcctg tcgtcttcgc catctcaata ccatttattg gcatgcaaac
      661 tcccgcaagc tctcacggaa agcaaacaac acaagtaagt actggtaatt ggagcaaaaa
      721 tgcagtggcc gctcggttag gatttttggc atgtggcctc tatgtaactt cctacttttt
      781 tagcataaaa aacaatcctt ttgctttatt gctcatttcg tctcacgaaa aaatgaatta
      841 tgtgcatcgt cgtctttctc aatatgcaat tatgatcggt gctattcacg gctttgcata
      901 cattggtctt gcagcgcaag gtaaaagggc acttcttact gcaagagtca ccattatcgg
      961 ttatgttatt ttagggttaa tggtaattat gatcgtaagc agcttgccat ttttccgtcg
     1021 aagattttac gaatggtttt ttgttttgca ccatatgtgc agcattggct ttttaattac
     1081 tatttggctc caccatcgaa ggtgtgtggt ttacatgaag gtctgtgttg ccgtgtacgt
     1141 gtttgatcgt ggctgcagaa tgctaagaag ctttttaaat cgttccaaat tcgatgttgt
     1201 tttggtggaa gacgatctca tctatatgaa agggcctcgt ccaaaaaaat ctttctttgg
     1261 attaccttgg ggtgctggaa accatatgta tattaatatt ccctctttaa gttattggca
     1321 aatacatcca ttcacgattg cgagcgttcc ttccgatgat tttatcgaac tatttgtcgc
     1381 agtaagggct ggtttcacaa agcgattagc caagaaggtc tcttccaaaa gtttgagtga
     1441 tgtctccgac atcaatatta gtgatgagaa gatagaaaaa aatggagacg ttggaattga
     1501 agtgatggaa agacattcgt tatcacagga agacttagtc ttcgaaagtt cggctgccaa
     1561 agtatcggta ctaatggatg gcccttacgg cccagtctca aacccataca aagattattc
     1621 ttacctcttt ttgtttgctg gaggtgttgg agtttcgtat atcttaccga ttattttgga
     1681 tactataaaa aagcaaagca gaaccgttca tataactttt gtttggtctg ctcgttcttc
     1741 ggctttattg aacatcgtgc ataagtcact ttgtgaagct gttcgttaca cagaaatgaa
     1801 tatcaacata ttctgccatt tgactaacag ctaccctgtc gaggaagtct cttctctaaa
     1861 ctctcaaagt gctagaaatt acagtttgca atacctaaat ggaaggcctg atgtaaatga
     1921 ttattttaaa gattttctgc atgcaactgg tactcaaaca gccgctcttg catcatgcgg
     1981 ctcggataag cttctgagac atcttaaaag ctgtgtcaat acacactcac cttccactgt
     2041 cgatctttat caacactatg aagaaatttg aaaaatttaa tgaacctcaa aactctcttt
     2101 taacggtgac aaattactga aagtgtacaa aatattttcc gattatttag atactaagtt
     2161 gcttgtaaaa caatcaatac ttccatagaa tcgtaaaatt ccatctaatt ttcgctttac
     2221 atttagaaaa gcatacatta attaatttga tggtttaagt tacgaaactg tattgtcttt
     2281 atctaacggt agcttatgtt gatccaaact ttggtaagaa aatcatacaa tcaagtt
//