From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Cloning vector pACYC177.
Date: 8 Jan 93 05:51:44 GMT
Distribution: bionet
Checksum: 26936 9

LOCUS       SYNACYC177   3941 bp ds-DNA   Circular  SYN       07-JAN-1993
DEFINITION  Cloning vector pACYC177.
ACCESSION   L08776
KEYWORDS    .
SOURCE      Artificial gene DNA; Cloning vector DNA; Transposon Tn3 DNA;
            Transposon Tn3 DNA; Cloning vector DNA; Transposon Tn903 DNA;
            Transposon Tn903 DNA; Transposon Tn903 DNA; Transposon Tn3 DNA; and
            Cloning vector DNA.
  ORGANISM  Artificial gene
            Artificial sequences; Genes.
REFERENCE   1  (bases 1 to 3941)
  AUTHORS   Gilbert,W.
  TITLE     Obtained from VecBase 3.0
  JOURNAL   Unpublished (1991)
  STANDARD  full automatic
COMMENT     VecBase accession number VB0109 COLLECTION  ATCC 37031
               COMMENT
              This sequence was obtained from B.E. Enger-Valk on floppy disc
            COMMENT
              pACYC177 contains the p15A replicon that is compatible with
            replicons
              pMB1 (present in pBR322 and most other cloning vectors) and
            ColE1. COMMENT  from Enger-Valk
              Gesequenced door mensen van B.R.L.
              Ingevoerd 22-10-84    Robert
              0 is het midden van de HinDII site GTT\GAC
               CROSSREFERENCE
              #parent
                GenBank(50):p15AOri, GenBank(50):Trn3, GenBank(50):Trn903,
                VecBase(3):pBR322
               PARENT
              Features of pACYC177 (3941 bp)
                residue     source
                 1- 762  3909-3148 (c) pBR322
                 1- 762  4196-4957     Tn3
               725- 762    38-   1     Tn3
               763-1588   201-1025     p15AOri
              1587-1815  2266-2038 (c) Tn903
              1587-3023   829-2265     Tn903
              2796-3023  1057- 830 (c) Tn903
              3018-3941  3272-4195     Tn3
              3487-3941  4363-3910 (c) pBR322
              Conflict (cfl) and Mutations (mut):
                         pACYC177  source
              cfl 1223-29 AGGGGGA  AGG-GGA 661-66     p15AOri
              cfl 2281          C  T         1523     Tn903
              cfl 3173-74     C-G  CCG    3427-29     Tn3
              cfl 3511-13     GCA  G-A    3766-67     Tn3
              cfl 3511-13     GCA  G-A    4348-49 (c) pBR322 FEATURE
              3765-3941    1-177  Ap-R; b-lactamase
                 1- 612  178-789  Ap-R; b-lactamase
              1920-2735    1-816  Km-R coding region SELECTION
              #resistance  Km, Ap.
FEATURES             Location/Qualifiers
     CDS             1..612
                     /product="beta-lactamase"
                     /codon_start=1
                     /translation="DAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAIT
                     MSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPAAM
                     ATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRG
                     IIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW"
     CDS             1920..2735
                     /codon_start=1
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLY
                     GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIP
                     GKTAFQVLEEYPDSGENIADALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGL
                     VDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVG
                     RVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             3765..3941
                     /product="beta-lactamase"
                     /codon_start=1
                     /translation="HPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMM
                     STFKVLLCGAVLSRV"
BASE COUNT     1026 a    894 c    993 g   1028 t
ORIGIN      
        1 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
       61 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
      121 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
      181 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
      241 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca
      301 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
      361 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
      421 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
      481 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
      541 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
      601 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
      661 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
      721 atcccttaac gtgagttttc gttccactga gcgtcagacc ccttaataag atgatcttct
      781 tgagatcgtt ttggtctgcg cgtaatctct tgctctgaaa acgaaaaaac cgccttgcag
      841 ggcggttttt cgaaggttct ctgagctacc aactctttga accgaggtaa ctggcttgga
      901 ggagcgcagt caccaaaact tgtcctttca gtttagcctt aaccggcgca tgacttcaag
      961 actaactcct ctaaatcaat taccagtggc tgctgccagt ggtgcttttg catgtctttc
     1021 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcggact gaacgggggg
     1081 ttcgtgcata cagtccagct tggagcgaac tgcctacccg gaactgagtg tcaggcgtgg
     1141 aatgagacaa acgcggccat aacagcggaa tgacaccggt aaaccgaaag gcaggaacag
     1201 gagagcgcac gagggagccg ccagggggaa acgcctggta tctttatagt cctgtcgggt
     1261 ttcgccacca ctgatttgag cgtcagattt cgtgatgctt gtcagggggg cggagcctat
     1321 ggaaaaacgg ctttgccgcg gccctctcac ttccctgtta agtatcttcc tggcatcttc
     1381 caggaaatct ccgccccgtt cgtaagccat ttccgctcgc cgcagtcgaa cgaccgagcg
     1441 tagcgagtca gtgagcgagg aagcggaata tatcctgtat cacatattct gctgacgcac
     1501 cggtgcagcc ttttttctcc tgccacatga agcacttcac tgacaccctc atcagtgcca
     1561 acatagtaag ccagtataca ctccgctagc gctgaggtct gcctcgtgaa gaaggtgttg
     1621 ctgactcata ccaggcctga atcgccccat catccagcca gaaagtgagg gagccacggt
     1681 tgatgagagc tttgttgtag gtggaccagt tggtgatttt gaacttttgc tttgccacgg
     1741 aacggtctgc gttgtcggga agatgcgtga tctgatcctt caactcagca aaagttcgat
     1801 ttattcaaca aagccacgtt gtgtctcaaa atctctgatg ttacattgca caagataaaa
     1861 atatatcatc atgaacaata aaactgtctg cttacataaa cagtaataca aggggtgtta
     1921 tgagccatat tcaacgggaa acgtcttgct cgaggccgcg attaaattcc aacatggatg
     1981 ctgatttata tgggtataaa tgggctcgcg ataatgtcgg gcaatcaggt gcgacaatct
     2041 atcgattgta tgggaagccc gatgcgccag agttgtttct gaaacatggc aaaggtagcg
     2101 ttgccaatga tgttacagat gagatggtca gactaaactg gctgacggaa tttatgcctc
     2161 ttccgaccat caagcatttt atccgtactc ctgatgatgc atggttactc accactgcga
     2221 tccccgggaa aacagcattc caggtattag aagaatatcc tgattcaggt gaaaatattg
     2281 ctgatgcgct ggcagtgttc ctgcgccggt tgcattcgat tcctgtttgt aattgtcctt
     2341 ttaacagcga tcgcgtattt cgtctcgctc aggcgcaatc acgaatgaat aacggtttgg
     2401 ttgatgcgag tgattttgat gacgagcgta atggctggcc tgttgaacaa gtctggaaag
     2461 aaatgcataa gcttttgcca ttctcaccgg attcagtcgt cactcatggt gatttctcac
     2521 ttgataacct tatttttgac gaggggaaat taataggttg tattgatgtt ggacgagtcg
     2581 gaatcgcaga ccgataccag gatcttgcca tcctatggaa ctgcctcggt gagttttctc
     2641 cttcattaca gaaacggctt tttcaaaaat atggtattga taatcctgat atgaataaat
     2701 tgcagtttca tttgatgctc gatgagtttt tctaatcaga attggttaat tggttgtaac
     2761 actggcagag cattacgctg acttgacggg acggcggctt tgttgaataa atcgaacttt
     2821 tgctgagttg aaggatcaga tcacgcatct tcccgacaac gcagaccgtt ccgtggcaaa
     2881 gcaaaagttc aaaatcacca actggtccac ctacaacaaa gctctcatca accgtggctc
     2941 cctcactttc tggctggatg atggggcgat tcaggcctgg tatgagtcag caacaccttc
     3001 ttcacgaggc agacctcagc gctcaaagat gcaggggtaa aagctaaccg catctttacc
     3061 gacaaggcat ccggcagttc aacagatcgg gaagggctgg atttgctgag gatgaaggtg
     3121 gaggaaggtg atgtcattct ggtgaagaag ctcgaccgtc ttggccgcga cacgccgaca
     3181 tgatccaact gataaaagag tttgatgctc agggtgtagc ggttcggttt attgacgacg
     3241 ggatcagtac cgacggtgat atggggcaaa tggtggtcac catcctgtcg gctgtggcac
     3301 aggctgaacg ccggaggatc ctagagcgca cgaatgaggg ccgacaggaa gcaaagctga
     3361 aaggaatcaa atttggccgc aggcgtaccg tggacaggaa cgtcgtgctg acgcttcatc
     3421 agaagggcac tggtgcaacg gaaattgctc atcagctcag tattgcccgc tccacggttt
     3481 ataaaattct tgaagacgaa agggcctcgt gcatacgcct atttttatag gttaatgtca
     3541 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
     3601 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     3661 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     3721 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     3781 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     3841 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     3901 cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt t
//