From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Citrus greening disease-associated bacterium-like organism
Date: 29 Sep 92 10:02:45 GMT
Distribution: bionet
Checksum: 44348     9

LOCUS       CGDRPL       3197 bp ds-DNA             BCT       29-SEP-1992
DEFINITION  Citrus greening disease-associated bacterium-like organism
            ribosomal protein and RNA polymerase (rplKAJL-rpoB) gene cluster.
ACCESSION   M94319
KEYWORDS    RNA polymerase; attenuator; nusG gene; ribosomal protein L1;
            ribosomal protein L11; ribosomal protein L12;
            ribosome binding site; rplA gene; rplJ gene; rplK gene; rplL gene;
            rpoB gene; terminator; transcription antitermination protein.
SOURCE      Citrus greening disease-associated bacterium-like organism DNA.
  ORGANISM  Citrus greening disease-associated bacterium-like organism
            Prokaryotae.
REFERENCE   1  (bases 1 to 3197)
  AUTHORS   Villechanoux,S., Garnier,M., Laigret,F., Renaudin,J. and Bove,J.M.
  TITLE     The genome of the non-cultured bacterial-like organism associated
            with citrus greening disease contains the nusG-rplKAJL-rpoBC gene
            cluster and the gene for a bacteriophage type DNA polymorphism
  JOURNAL   Curr. Microbiol. (1992) In press
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     CDS             <1..213
                     /product="transcription antitermination protein"
                     /gene="nusG"
                     /note="putative"
                     /codon_start=1
                     /translation="NQVEAAVQRPVSSVFFEVGERVCVSDGPFASFNGIVKNVDEEKS
                     RVHVEVVIFGRVTPVELAYNQVEKIV"
                     /citation=[1]
     -35_signal      218..224
                     /gene="rplK"
     terminator      225..251
                     /gene="nusG"
     -10_signal      244..250
                     /gene="rplK"
     RBS             290..297
                     /gene="rplK"
     CDS             304..732
                     /product="ribosomal protein S11"
                     /gene="rplK"
                     /note="putative"
                     /codon_start=1
                     /translation="MAKVVSRIVKLQIESGSAKPSPPVGPAIGQAGIPIMAFCKAFNA
                     ATEGMEKGIPIPTTVTCYKDKSFTFTMSQPPVSFFLKKEVGIKSGSKLPGKESCGSIT
                     RENIRKIAQLKMQDMGAIDIEGAMRMVEGSACSMGISVVD"
     RBS             720..726
                     /gene="rplA"
     CDS             734..1432
                     /product="ribosomal protein L1"
                     /gene="rplA"
                     /note="putative"
                     /codon_start=1
                     /translation="MVSKISKRMQQISQGIDRSALYGLSDAVVMLKERATARFDETVE
                     IAMNLGIDPRHANQMVRGVVTMPNGTGVNVRVAVFATSSKADEAREAGADIVGGEDLF
                     EIVKGGQIDFDRCIATPDMMPLVGRLGRILGPRGIMPNLRVGTVTTDVATAVRESKSG
                     AVDFRSEKAGIIHAGIGKVSFENKKIEENVLAFVSAVVKAKPSVAKGDYVKRVTLSST
                     MGCGIKVDLSSFSV"
                     /citation=[1]
     RBS             1591..1599
                     /gene="rplJ"
     CDS             1605..2123
                     /product="ribosomal protein L11"
                     /gene="rplJ"
                     /note="putative"
                     /codon_start=1
                     /translation="LNRQGKSVEISELSKIFSSSGSIVVAHYKGISVAQIKDLRKKMR
                     EAGGGVKVAKNRLVKIAIRDTSIRGISDLFVGQSLIVYSDSPVIAPKISVSFSNDNNE
                     FRVLGGVVEKGVLNQDSIKQIASLPDLEGIRAGIISAIQSNATRLVRLLGTPQTQVVR
                     AISAFVDKNQQG"
                     /citation=[1]
     RBS             2157..2164
                     /gene="rplL"
     CDS             2172..2540
                     /product="ribosomal protein L12"
                     /gene="rplL"
                     /note="putative"
                     /codon_start=1
                     /translation="MSNIESIVEKLSSLTLIEAAELSKRLEKEWGVSASAPVSVVAPV
                     AAEAGSAASEKTEFEVVLKGFDDPKKKIAVIKEVRAITDLGLKEAKELVESAPKSLKT
                     GLSKDEANEMKKKLEDAGAT"
                     /citation=[1]
     attenuator      2575..2596
                     /gene="rpoB"
     RBS             2599..2605
                     /gene="rpoB"
     CDS             2664..>3197
                     /product="RNA polymerase beta-subunit"
                     /gene="rpoB"
                     /note="putative"
                     /codon_start=1
                     /translation="MAKGVVFNGLGRVRKFFGKNPEIIDIPDLIEVQKASYDHFLMMN
                     IAPDERPNEGLQAAFKSVFPITAFSGAAMLEFVSYEFDPPKFDVDDCLWRDLTYAVPL
                     KITLRLIVFDVDEFTGAKSIKDIKEQSIYMGDLPLMTKDGTFVIKGTQRIVVSQLHRS
                     PGIHFDHDKGRASLSGKL"
                     /citation=[1]
BASE COUNT      851 a    457 c    813 g   1076 t
ORIGIN
        1 aatcaagttg aagcagctgt ccaaaggcct gtatcgtctg ttttcttcga ggttggtgag
       61 cgggtttgtg tctctgatgg tccgtttgct tcttttaatg gtattgtaaa gaatgtcgat
      121 gaagaaaaat ctcgcgttca tgtagaagtt gtgatttttg gtcgtgtcac accagtagag
      181 ttagcataca atcaagttga gaagatcgta tgatattttg atttcggtca tgagtgggcg
      241 tattattatt gcttttgtgt ttgttttata tagtaggttg gttgtttttt agaaaggcta
      301 gggatggcta aagtagtttc tcgtatagtt aagttgcaga tagagtcggg ttctgcaaag
      361 ccttcgccgc cggttggtcc tgcaattgga caggctggta ttcccattat ggcgttttgt
      421 aaggcgttta atgcggcaac tgagggtatg gaaaagggta ttcccatccc aactactgtg
      481 acttgttata aagataagtc ttttactttt acgatgagtc agccacctgt aagctttttt
      541 cttaaaaaag aggtggggat taagtcggga tctaaacttc ctggtaagga gtcttgcggt
      601 tcgattactc gggaaaatat tagaaaaatt gcacagttaa agatgcaaga tatgggggct
      661 attgatattg aaggggctat gcgtatggtg gaaggttcgg cctgttcaat gggtataagt
      721 gtggtggatt aatatggtgt ctaaaatttc gaaacgtatg cagcagattt ctcaggggat
      781 tgatcgttca gcgttatatg ggttaagtga tgctgtggtt atgcttaaag agcgtgctac
      841 ggctaggttt gatgaaacag ttgagatagc aatgaatcta ggcattgatc ctcggcatgc
      901 taatcagatg gttcgtggcg ttgttactat gcctaatggc acgggggtaa atgttcgcgt
      961 ggcggttttt gctacatctt cgaaggcgga tgaagctagg gaagcagggg ctgatattgt
     1021 tggaggagaa gatcttttcg aaattgttaa aggcgggcag atagattttg accgttgtat
     1081 tgctacaccg gatatgatgc ctttagttgg aaggttgggt aggattttag gtcctcgtgg
     1141 tattatgcct aatttgaggg ttggtacggt gacaacagat gttgctactg ctgttcgaga
     1201 gtctaagagt ggtgcggttg attttcgttc tgaaaaagca gggattattc atgcggggat
     1261 aggtaaggtt tctttcgaga ataaaaaaat agaggaaaat gttttggctt ttgttagtgc
     1321 tgttgtaaag gcgaaacctt ctgttgcgaa aggtgattac gtaaagcgtg ttactttgtc
     1381 atcgactatg ggatgtggta taaaggttga cctttcgagt ttctctgttt aatactcttg
     1441 tttagttgtt ttttgtgtgg attccttttt cgctatcgga tcgcttcttt tttgtaaggg
     1501 atgcgttagg atttttgttc ttcttcgaaa tcaagatatg aaaatatttt cttggtatag
     1561 atataggaaa aggaatgggt atatttgtca tctggagatg aaagttgaat agacaaggaa
     1621 agagcgtaga aatttctgaa ttaagtaaga ttttttcttc ttctggatca attgttgttg
     1681 cacattataa gggaattagt gttgcgcaaa ttaaagatct tcggaaaaag atgcgggaag
     1741 ctggtggagg tgtaaaagtt gccaaaaatc gtctcgtcaa gattgctatc cgtgatacta
     1801 gtattagagg aatatctgat cttttcgttg ggcagtctct aattgtctat tcggatagtc
     1861 ctgttattgc tcctaaaatt tcggttagct tttcaaatga caataatgaa tttagagttc
     1921 ttggtggggt tgtagagaag ggcgtcctta atcaagattc tatcaagcaa attgcttctt
     1981 tacccgatct tgagggtatt cgagctggta tcataagtgc tatccaatct aatgcaacta
     2041 gattggttag acttcttggt acgccacaga ctcaagttgt tcgtgctatt tctgcttttg
     2101 tggataaaaa tcagcaaggt tagttctgtt catcgttaaa aactaagata aaaacaaagg
     2161 aaatatgtga tatgtccaat attgaatcaa ttgttgaaaa attgtcctct cttactctta
     2221 ttgaagctgc ggaactttca aaaagattag aaaaggaatg gggtgtttct gcttctgcgc
     2281 ccgtttccgt tgttgcgcct gttgctgcag aggcgggttc tgctgcatct gaaaagactg
     2341 aatttgaagt agtattgaaa ggttttgatg atcctaagaa aaaaatagct gtcatcaaag
     2401 aggtgcgcgc aattactgat ttagggctta aagaggctaa ggagcttgta gaaagtgctc
     2461 ctaagagctt aaaaacaggg cttagtaaag atgaggctaa tgaaatgaag aagaaacttg
     2521 aagatgctgg tgcaacgtag aattacgcat gtttatggta tgattgagtt ttaaaggctc
     2581 ctttacaaag gggcttcgtt tttaggaaaa aaatttattt tcctgctttg tattttgtca
     2641 cgaatttgtt atgaggagaa acgatggcaa aaggcgttgt gttcaatggt ctcgggcgtg
     2701 tgcgcaagtt ttttggaaaa aatcctgaga taattgacat acctgatctc attgaagttc
     2761 agaaggcatc ttatgatcat tttttgatga tgaacattgc tcccgatgag cgtcctaatg
     2821 aaggtttaca ggctgctttt aagtctgttt ttcctattac ggctttttct ggggctgcga
     2881 tgcttgaatt tgtatcttat gaatttgatc ccccaaagtt tgatgtagat gattgtttat
     2941 ggcgtgattt gacgtatgcc gtaccattga agataactct gcgtttgatc gtatttgatg
     3001 tagatgagtt cactggagct aagtctatca aagatataaa agagcaaagt atttatatgg
     3061 gcgatcttcc tcttatgact aaagacggaa ctttcgttat taagggtact cagcgtatag
     3121 ttgtttccca gttacatcgt tctcctggta tacattttga tcatgataaa ggaagagcta
     3181 gtttatcggg caagctt
//