From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Plasmid R386 (from E. coli) replication protein A (repA) gene,
Date: 10 Sep 92 10:35:30 GMT
Distribution: bionet
Checksum: 46414     4

LOCUS       R36REPAA     1525 bp ds-DNA             BCT       10-SEP-1992
DEFINITION  Plasmid R386 (from E. coli) replication protein A (repA) gene,
            complete cds.
ACCESSION   L01254
KEYWORDS    repA gene; replication protein A.
SOURCE      Plasmid R386 DNA.
  ORGANISM  Plasmid R386
            Prokaryotae.
REFERENCE   1  (bases 1 to 1525)
  AUTHORS   Gibbs,M.D., Spiers,A.J. and Bergquist,P.L.
  TITLE     RepFIB: A basic replicon of large plasmids
  JOURNAL   Unpublished (1992)
  STANDARD  full staff_entry
FEATURES             Location/Qualifiers
     CDS             276..1253
                     /note="central protein required for replication"
                     /product="replication protein A"
                     /gene="repA"
                     /codon_start=1
                     /translation="MDKSSGELVTLTPNNNNTVQPVALMRLGVFVPTLKSLKNSKKNT
                     LSRTDATEELTRLSLARAEGFDKVEITGPRLDMDNDFKTWVGIIHSFARHNVIGDKVE
                     LPFVEFAKLCGIPSSQSSRSLRERISPSLKRIAGTVISFSRTDEKHTREYITHLVQSA
                     YYDTERDIVQLQADPRLFELYQFDRKVLLQLKAINALKRRESAQALYTFIESLPRDPA
                     PISLARLRARLNLKSPVFSQNQTVRRAMEQLREIGYLDYTEIQRGRTKFFCIHYRRPR
                     LKAPNDESKENPLPPSPAEKVSPEMAEKLALLEKLGITLDDLEKLFKSR"
BASE COUNT      391 a    381 c    361 g    392 t
ORIGIN
        1 gggccgggag cacgatcaca taagctgtgg catgttactg ataaactgta acatgctaat
       61 gataagctgt attcagtaat ccatatactg aagtaagtta atgacataaa ctatggtcag
      121 tatgccagac tcagttgtta aatacaggct gcaggttttt cttcagtcag ttagcggagt
      181 tctgacacac gattttctgt ttattctttt actgtccaca ggcagaaggc tttctggaaa
      241 acgaaaattc agacatcaaa aaactgttcg gcgaggtgga taagtcgtcc ggtgagctgg
      301 tgacactgac accaaacaat aacaacaccg tacaacctgt ggcgctgatg cgtctgggcg
      361 tttttgtacc gacccttaaa tcactgaaga acagtaaaaa aaatacactg tcacgtactg
      421 atgccacgga agagctgact cgtctttccc tggcccgtgc tgagggattc gataaggttg
      481 agatcaccgg cccccgcctg gatatggata acgatttcaa gacctgggtg gggatcattc
      541 attcctttgc ccgccataac gtgattggtg acaaagttga actgcctttt gttgagtttg
      601 caaaactgtg tggtatacct tcaagccagt catcccgcag cctccgtgag cgcatcagcc
      661 cttccctgaa gcgcattgcc ggtaccgtga tctcgttttc ccgtaccgat gagaagcaca
      721 cccgggaata catcacccat ctggtacagt cagcctacta cgatactgag cgggatattg
      781 ttcagttaca ggctgatccc cgtctgtttg aactgtacca gtttgacaga aaagtccttc
      841 tccagcttaa ggcgattaat gccctgaagc gacgggagtc cgcccaggca ctctacacct
      901 ttatagagag cctgccccgg gatccggcac cgatatcgct ggcgcggctg cgtgcacgcc
      961 tcaatctgaa gtctcctgta ttttcccaga accagacggt cagacgggca atggagcagt
     1021 tgcgcgagat tggatatctt gattacacgg agatccagcg ggggcggaca aaattcttct
     1081 gtattcacta ccggcgtccc cggttaaaag cgccgaatga tgagagtaag gaaaatccgt
     1141 tgccaccttc acctgcggaa aaagtcagtc cggagatggc ggagaaactt gccctgcttg
     1201 aaaaactggg catcacactg gatgacctgg aaaaactctt caaatcccgc tgaacataaa
     1261 ctgtagtcag tgaagagtgc tcctttactg actacagctt atattatcag gtgcagtgag
     1321 tggtctgctc actgctgttt atattcagtt tcctgcagtg ctgcctgtag ctgagctgcc
     1381 atctgcctgt cccttacgta agtgatcccg taacctgatg ctgaggcatt gctcccttca
     1441 taaaacatga cttactcact acagcttata tacatgctcc agcttatgtt atgcctgttc
     1501 tgctgaccac agcttatata aggaa
//