HDTenEightyP/NetNews huggingface dataset:
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human immunodeficiency virus type 2 (22POLB1) polymerase fragment.
Date: 18 Aug 92 10:01:16 GMT
Distribution: bionet
Checksum: 17813     2

LOCUS       HIV2POLB1     666 bp ss-RNA             VRL       18-AUG-1992
DEFINITION  Human immunodeficiency virus type 2 (22POLB1) polymerase fragment.
ACCESSION   M87135
KEYWORDS    polymerase.
SOURCE      Human immunodeficiency virus type 2 uncultured blood mononuclear
            cells (5/89 sample) RNA.
  ORGANISM  Human immunodeficiency virus type 2
            Viridae; ss-RNA enveloped viruses; Positive strand RNA virus;
            Retroviridae; Lentivirinae.
REFERENCE   1  (bases 1 to 666)
  AUTHORS   Gao,F., Yue,L., White,A.T., Pappas,P.G., Barchue,J., Hanson,A.P.,
            Greene,B.M., Sharp,P.M., Shaw,G.M. and Hahn,B.H.
  TITLE     Human infection by genetically-diverse SIVsm-related HIV-2 in West
            Africa
  JOURNAL   Nature (1992) In press
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     misc_feature    1..666
                     /note="PCR derived HIV-2 polymerase fragment"
                     /note="putative"
                     /citation=[1]
BASE COUNT      245 a    121 c    158 g    142 t
ORIGIN
        1 actagtaaac agaatcatag aagagttaat aaggaaggaa gctgtttaca tcagctgggt
       61 gccagctcac aagggtctcg gaggaaatca agaagtagac cacctagtaa gtcaaggaat
      121 taggcaagta tgattcttag aaaaaataga accagcacag gaagagcatg aaagatttca
      181 tagcaatgcc aaagagctga cacacaaatt tggtatacct aacttagtgg ctaaacagat
      241 agtaaatact tgtcatgtgt gtcaacagaa aggagaggcc atacatgggc aagtaaatgc
      301 agatttagga acatggcaaa tggactgtac ccatttagaa ggaaaagtta tcatagtagc
      361 agtccatgtg gccagtggct ttatagaagc agaggtaatc ccacaagaaa ctggcagaca
      421 aacagctctt tttctgttaa aattggcagc aagatggcct attacccacc tacacacaga
      481 taatggtgcc aatttcactt cacaggaagt aaaaatggtg gcgtggtggg taggcgtgga
      541 acataccttt ggagtacctt acaacccaca gagtcaggga gtagtagagg ccatgaacca
      601 tcatctgaaa aaccaaatag atagaatcag agagcaggca aacacaatgg agaccatagt
      661 cttgat
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Homo sapiens 21 kDa protein gene, complete cds, clone D4S234.
Date: 14 Aug 92 10:00:38 GMT
Distribution: bionet
Checksum: 39496     3

LOCUS       HUMPPIB      1083 bp ds-DNA             PRI       14-AUG-1992
DEFINITION  Homo sapiens 21 kDa protein gene, complete cds, clone D4S234.
ACCESSION   M98529
KEYWORDS    21 kDa protein.
SOURCE      Homo sapiens DNA.
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 1083)
  AUTHORS   Carlock,L., Vo,T., Wisniewski,D. and Lorincz,M.
  TITLE     The Identification of a neuron-specific gene that maps adjacent to
            the Huntington's Disease marker D4S10 that shows homology to
            protein phosphatase inhibitors
  JOURNAL   Unpublished (1992)
  STANDARD  full staff_entry
FEATURES             Location/Qualifiers
     CDS             223..780
                     /function="unknown"
                     /product="21 kDa protein"
                     /gene="21 kDa protein"
                     /note="putative"
                     /codon_start=1
                     /translation="MVKLGNNFAEKGTKQPLLEDGFDTIPLMTPLDVNQLQFPPPDKV
                     VVKTKTEYEPDRKKGKARPPQIAEFTVSITEGVTERFKVSVLVLFALAFLTCVVFLVV
                     YKVYKYDRACPDGFVLKNTQCIPEGLESYYAEQDSSAREKFYTVINHYNLAKQSITRS
                     VSPWMSVLSEEKLSEQETEAAEKSA"
     polyA_signal    923..928
                     /gene="21 kDa protein"
BASE COUNT      258 a    312 c    277 g    236 t
ORIGIN
        1 ataatctaga aatacacagc accacccgac ccccgcatcg ggccgtgacc accgcgtccc
       61 cacgagccct ccccgagacg aagcggggcc ggggagctcg cggacgccgg gacgccggtg
      121 ggtgtgggtg cccacttccc ccccgccccg ccccgggtct tgcttgtggt gactcccccc
      181 ggccctcccg ccgcaggctg cagcctcgga gctcccggaa cgatggtgaa gttggggaac
      241 aatttcgcag agaagggcac caagcagccg ctgctggagg atggcttcga caccattccc
      301 ctgatgacgc ccctcgatgt caatcagctg cagttcccgc ccccggataa ggtggtcgtg
      361 aaaactaaga ccgagtatga acctgaccgc aagaaaggga aagcacgtcc tccccaaatt
      421 gctgagttca ccgtcagcat cacggagggt gtcaccgaga ggtttaaggt ctccgtgttg
      481 gtcctcttcg ccctggcctt cctcacctgc gtcgtcttcc tggttgtcta caaggtgtac
      541 aagtatgacc gcgcctgccc cgatgggttc gtcctcaaga acacccagtg catcccagaa
      601 ggcttggaga gctactacgc ggagcaagac tccagtgccc gggagaaatt ttacacagtc
      661 ataaaccact acaacctggc caagcagagc atcacgcgct ccgtatcgcc ctggatgtca
      721 gttctgtcag aagagaagct gtccgagcag gagactgaag cggctgagaa gtcagcttag
      781 cgggatgggc aagttcctta caatgtgtca cttgcaaata acaaagggac tttgagggac
      841 atttcattaa atataattac tgatacttta gaggttactc atttacggtg caattgcttc
      901 tgtttgctaa tgctgctttg caaataaaac ttgctgccga ccacccacgg gcataaaatc
      961 aagtgcattt cagcattgcc taaagagctc tgacaccact tttcatgtta agatcttcat
     1021 ttagctcctt tactgggatt tattggatgc tgtaaaaaaa taaatttaca ctggatatgc
     1081 gaa
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Mouse T cell receptor rearranged aplpha-chain variable region,
Date: 15 Aug 92 10:02:37 GMT
Distribution: bionet
Checksum: 36842     2

LOCUS       MUSTCRA2C     318 bp ss-mRNA            ROD       15-AUG-1992
DEFINITION  Mouse T cell receptor rearranged aplpha-chain variable region,
            N_region, joining region, and constant region mRNA.
ACCESSION   M98572
KEYWORDS    T-cell receptor; alpha-subunit receptor; constant region;
            joining region; variable region.
SOURCE      Mus musculus (strain BALB/cKh) adult spleen cDNA to mRNA.
  ORGANISM  Mus musculus
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Rodentia; Myomorpha; Muridae; Murinae.
REFERENCE   1  (bases 1 to 318)
  AUTHORS   Solheim,J.C., Martinko,J.M., Alexander-Miller,M. and Connolly,J.M.
  TITLE     Biased T Cell Receptor Usage by Ld-restricted Tum-Peptide-specific
            Cytotoxic T Lymphocyte Clones
  JOURNAL   Unpublished (1992)
  STANDARD  full staff_entry
BASE COUNT      100 a     77 c     66 g     75 t
ORIGIN      Chromosome 14
        1 acagcaagtg gggaaatagt tttccttatt cgtcaggact cttacaaaaa ggaaaatgca
       61 acagtgggtc attattctct gaactttcag aagccaaaaa gttccatcgg actcatcatc
      121 accgccacac agattgagga ctcggcagta tatttctgtg ctatgagaga ggggaactat
      181 ggaaatgaga aaataacttt tggggctgga accaaactca ccattaaacc caacatccag
      241 aacccagaac ctgctgtgta ccagttaaaa gatcctcggt ctcaggacag caccctctgc
      301 ctgttcaccg actttgac
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human immunodeficiency virus type 2 (FOENVA13) envelope protein
Date: 18 Aug 92 10:01:28 GMT
Distribution: bionet
Checksum: 09133     2

LOCUS       HIVENVA13     453 bp ss-RNA             VRL       18-AUG-1992
DEFINITION  Human immunodeficiency virus type 2 (FOENVA13) envelope protein
            fragment.
ACCESSION   M87069
KEYWORDS    envelope protein.
SOURCE      Human immunodeficiency virus type 2 uncultured blood mononuclear
            cells (11/89 sample) RNA.
  ORGANISM  Human immunodeficiency virus type 2
            Viridae; ss-RNA enveloped viruses; Positive strand RNA virus;
            Retroviridae; Lentivirinae.
REFERENCE   1  (bases 1 to 453)
  AUTHORS   Gao,F., Yue,L., White,A.T., Pappas,P.G., Barchue,J., Hanson,A.P.,
            Greene,B.M., Sharp,P.M., Shaw,G.M. and Hahn,B.H.
  TITLE     Human infection by genetically-diverse SIVsm-related HIV-2 in West
            Africa
  JOURNAL   Nature (1992) In press
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     misc_feature    1..453
                     /note="PCR derived HIV-2 envelope fragment"
                     /gene="env"
                     /note="putative"
                     /citation=[1]
BASE COUNT      159 a     77 c    106 g    111 t
ORIGIN
        1 gacgtggtca agagacaaca agaattgttg cgactgaccg tctgggggac taaaaacctc
       61 cagactagag tcactgctat cgagaagtac ctaaaggacc aggcgcagct aaattcatgg
      121 ggatgcgcgt tcagacaggt ctgccacacc actgtagaat ggccaaatag tactctcaca
      181 cctgactgga acaatatgac ttggcaggag tgggaaagac aggttgattt cctagaggca
      241 aatataacac aattattaga ggaagcacaa attcagcagg agaaaaacat gtatgagtta
      301 caaaaactaa ataactggga tatatttggc aactggtttg accttacttc ttggattaag
      361 tatatacagt atggagtctt catagtagta ggtataatac tgttaagaat aataatctat
      421 gtagtacaaa tgttagctaa gttaagtaag gga
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human chromosome 4 sequence-tagged site STS4-343
Date: 21 Aug 92 10:09:10 GMT
Distribution: bionet
Checksum: 11542     3

LOCUS       HUM4STS343    455 bp ds-DNA             PRI       21-AUG-1992
DEFINITION  Human chromosome 4 sequence-tagged site STS4-343
ACCESSION   L00804
KEYWORDS    STS; human chromosome 4; sequence tagged site.
SOURCE      Homo sapiens, clone p4-253 from a chromosome 4 plasmid library;
            vector pBluescript II KS + (Stratagene)
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 455)
  AUTHORS   Goold,R.D., diSibio,G., Dugaiczyk,A., Smith,K.A., Xu,H.,
            Magrane,G., Lang,D.B., Cox,D.R., Masters,S.B. and Myers,R.M.
  TITLE     Sequence-Tagged Sites for Human Chromosome 4
  JOURNAL   Unpublished (1992) see COMMENT for author address
  STANDARD  full automatic
COMMENT     PCR components: 25 ng of human genomic DNA, 10 pmol of each
            oligonucleotide, 200 micro-M dNTPs, 0.25 U Taq  polymerase (Cetus)
            in 10 micro-l of 50 mM KCl-20 mM  Tris-HCl, pH 8.3 (at room temp),
            2.5 mM MgCl-2.  Initial denaturation at 94degC for 1.5 min, then 30
            cycles of  94degC for 15 sec, 62degC for 23 sec, and 72degC for 30
            sec, followed by a final extension at 72degC for 3.5 min, using a
            Perkin-Elmer 9600 thermocycler. PCR-amplified product size 193 bp.
            STS sequence contains a simple sequence tandem repeat (CA).
            Sequence submitted by:
            Human Genome Mapping Center
            Box 0925
            University of California San Francisco
            San Francisco, CA 94143-0925  USA
            Phone: (415) 502-1612   Fax: (415) 476-8391
            e-mail: hgmcprobes@cgl.ucsf.edu
FEATURES             Location/Qualifiers
     misc_feature    7..30
                     /label=Primer1
     misc_feature    complement(173..199)
                     /label=Primer2
BASE COUNT      161 a     80 c     64 g    138 t     12 others
ORIGIN
        1 gatccactgt gcccagcctt tgttttctta ggacatatac aatacacaca ctcacacaca
       61 cacacacaca cacacacaca cctttccaat aagttgtagt agagaagaga aaaaaaacaa
      121 agaaacgtaa taaccaatgc cagaaatttc tggtgactat agatttcttt ttcattcaat
      181 ccattctaat tggtcataga atatttttat caagaaactt aatcatgacc tgagtttgnt
      241 ttatatcaaa gattagtaga aatgacatat ttagaaaaca aatttttatt tatattttnc
      301 cctgtaacat acattgtntg ggtaggatac atattcaaaa tgttttctgt ttctgcacag
      361 naaagtctta agaaaagtgc tgggaaatat ntccaccnna gaaatcaata aaaggtgggt
      421 acctnnncaa atgtgtacaa tatnttctgn cttgg
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Arabidopsis thaliana recombination and DNA-damage resistance
Date: 12 Aug 92 10:00:24 GMT
Distribution: bionet
Checksum: 28297     3

LOCUS       ATHDRT111X   1364 bp ss-mRNA            PLN       12-AUG-1992
DEFINITION  Arabidopsis thaliana recombination and DNA-damage resistance
            protein (DRT111) mRNA, complete cds.
ACCESSION   M98455
KEYWORDS    DNA-damage resistance protein; recombination protein.
SOURCE      Arabidopsis thaliana (strain Columbia) (library: Lambda-YES-R) cDNA
            to mRNA.
  ORGANISM  Arabidopsis thaliana
            Eukaryota; Plantae; Embryobionta; Magnoliophyta; Magnoliopsida;
            Dilleniidae; Capparales; Brassicaceae.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Pang,Q., Hays,J.B. and Rajagopal,I.
  TITLE     Two plant cDNAs from Arabidopsis thaliana that partially complement
            deficiencies in recombination and DNA-damage resistance in E. coli
            mutants lacking recombination-intermediate-resolution activities
  JOURNAL   Unpublished (1992)
  STANDARD  full staff_entry
FEATURES             Location/Qualifiers
     5'UTR           1..60
     3'UTR           1213..1364
BASE COUNT      420 a    261 c    362 g    321 t
ORIGIN
        1 cagatctttc caattgaaag cgaaccgtga attttgagtc tctttaccgg aggagaaaaa
       61 atgcttggtg gattatacgg agatcttcct ccaccgaccg atgatgagaa acccagtgga
      121 aactcctctt ccgtctggtc acgcagtacc aaaatggctc cacctacact tcgcaaacca
      181 ccagcttttg ctcccccgca aacaatctta agacctctca acaaacctaa acctatcgtt
      241 tcggctccgt acaagcctcc tcctccttca tcgcaatcgg tgcttattcc ggcgaacgaa
      301 tcagcacctt cgcatcagcc tgcattggtt ggtgtgactt catcggtgat tgaagagtac
      361 gatccagcga gacctaacga ttacgaggag tataagaggg agaagaagag gaaagctacg
      421 gaagctgaga tgaaacgaga gatggataag agaaggcaag tgtatccgga aagagatatg
      481 agagaaagag aagagaggga gaggagagag agagagataa cagtgatcct ctcggttgat
      541 atctccggtg aggaacgtgg aagagaccct gctagagtag tggtggaagt gttgggaagg
      601 gaagatcctc gtctcctccc tgggaatgtt gatgggttta gtattgggaa atcgaagccg
      661 agtgggttag gagtaggagc aggtggacag atgacacctg ctcagaggat gatgccgaag
      721 atgggatgga aacaaggaca agggcttggg aaatcagagc aagggattcc tacgccattg
      781 atggctaaga agactgatcg tagagctggt gtcattgtca atgctagtga gaataaatct
      841 tcttcggcgg agaagaaggt tgttaagagt gttaatatca atggtgaacc aaccagggtt
      901 ttgcttctta gaaacatggt tgggccagga caagtagatg atgagctaga agatgaggta
      961 ggaggcgagt gtgccaaata cggtacagtc actcgtgtac tgatattcga gatcactgaa
     1021 ccgaacttcc ctgtacacga agcagtaaga atctttgttc agttttcaag acccgaggaa
     1081 acaactaaag cccttgtaga cctcgatggg agatactttg gaggaagaac cgtacgtgca
     1141 acgttctatg atgaagagaa attcagtaag aacgagttgg ctccagttcc aggtgaaatc
     1201 cctggctatt aataactcct aagacaaaga agaaacttca tcttctagga gattcttgtt
     1261 agagaaagaa atcatagact gaggttttaa aaacatttgt aaagtactta gaaatcttgg
     1321 gttatctctt tattgttgtt caataaagct tcacaaggat caag
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human chromosome 4 sequence-tagged site STS4-178
Date: 21 Aug 92 10:07:23 GMT
Distribution: bionet
Checksum: 16267     3

LOCUS       HUM4STS178    442 bp ds-DNA             PRI       21-AUG-1992
DEFINITION  Human chromosome 4 sequence-tagged site STS4-178
ACCESSION   M95254
KEYWORDS    STS; human chromosome 4; sequence tagged site.
SOURCE      Homo sapiens, clone C4-159 from Los Alamos National Laboratory
            chromosome 4 cosmid library, plate 4-1-2R, pos 9A; vector SuperCos
            1 (Stratagene)
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 442)
  AUTHORS   Goold,R.D., diSibio,G., Dugaiczyk,A., Smith,K.A., Xu,H.,
            Magrane,G., Lang,D.B., Cox,D.R., Masters,S.B. and Myers,R.M.
  TITLE     Sequence-Tagged Sites for Human Chromosome 4
  JOURNAL   Unpublished (1992) see COMMENT for author address
  STANDARD  full automatic
COMMENT     PCR components: 25 ng of human genomic DNA, 10 pmol of each
            oligonucleotide, 200 micro-M dNTPs, 0.25 U Taq  polymerase (Cetus)
            in 10 micro-l of 50 mM KCl-20 mM  Tris-HCl, pH 8.3 (at room temp),
            2.5 mM MgCl-2.  Initial denaturation at 94degC for 1.5 min, then 30
            cycles of  94degC for 15 sec, 63degC for 23 sec, and 72degC for 30
            sec, followed by a final extension at 72degC for 3.5 min, using a
            Perkin-Elmer 9600 thermocycler. PCR-amplified product size 199 bp.
            Sequence submitted by:
            Human Genome Mapping Center
            Box 0925
            University of California San Francisco
            San Francisco, CA 94143-0925  USA
            Phone: (415) 502-1612   Fax: (415) 476-8391
            e-mail: hgmcprobes@cgl.ucsf.edu
FEATURES             Location/Qualifiers
     misc_feature    80..103
                     /label=Primer1
     misc_feature    complement(256..278)
                     /label=Primer2
BASE COUNT      114 a     88 c     85 g    123 t     32 others
ORIGIN
        1 tgnnnaactt actcnctnat tcacaagnaa cagcatgggg aaaccagtcc tatgattcaa
       61 ttgtatacac ctggncccac ccttgacaca tggagattat tacaattcaa ggtgagattt
      121 gggtcgggac atagagccag atcatatcgt tccatgcctg acccctcaaa atatcatgtc
      181 ctcctacatc aaggcaagtc ccttctgtct atgaggcagt aaaatcaaac acaagtgagt
      241 tacttttttn gatacaatgg gggtgcaggc attgcataan tgcacccatt ccaaatgggn
      301 gnagttngtc anaacaaagg ggntncaggg ttccattgcn aattctgaaa ttccaattna
      361 ggtgggtnat taanggtttn nngttttcaa aatnntcttc tttnncttct ttngtcactc
      421 atnctggggn ncanttgttg tn
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Lentinus kauffmannii ribosomal RNA (35LR0R) large subunit, partial.
Date: 19 Aug 92 10:01:56 GMT
Distribution: bionet
Checksum: 00979     2

LOCUS       LENRRLSAV     183 bp ss-rRNA            RNA       19-AUG-1992
DEFINITION  Lentinus kauffmannii ribosomal RNA (35LR0R) large subunit, partial.
ACCESSION   M98656
KEYWORDS    large subunit ribosomal RNA.
SOURCE      Lentinus kauffmannii (library: Duke culture collection 619) cDNA to
            rRNA.
  ORGANISM  Lentinus kauffmannii
            Eukaryota; Fungi; Eumycota; Basidiomycotina; Hymenomycetes;
            Agaricales; Tricholomataceae.
REFERENCE   1  (bases 1 to 183)
  AUTHORS   Hibbett,D.S. and Vilgalys,R.
  TITLE     Phylogenetic relationships of the basidiomycete genus Lentinus
            inferred from molecular and morphological characters
  JOURNAL   Unpublished (1992)
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     rRNA            <1..>183
                     /note="Aligns to region between positions 70 to 306
                     inSaccharomyces cerevisiae large subunit nuclear rRNA."
                     /product="large subunit ribosomal RNA"
                     /citation=[1]
BASE COUNT       38 a     37 c     56 g     52 t
ORIGIN
        1 ggtctttggc cgtccgagtt gtattctgga gaagtgcttt ccgcgctgga ccgtgtataa
       61 gtctcttgga acagagcgtc atagagggtg agaatcccgt ctttgacacg gactaccagt
      121 gctttgtgat gcgctctcaa agagtcgagt tgtttgggaa tgcagctcaa atgggtggta
      181 atc
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human chromosome 4 sequence-tagged site STS4-476
Date: 21 Aug 92 10:10:46 GMT
Distribution: bionet
Checksum: 49982     3

LOCUS       HUM4STS476    424 bp ds-DNA             PRI       21-AUG-1992
DEFINITION  Human chromosome 4 sequence-tagged site STS4-476
ACCESSION   L00881
KEYWORDS    STS; human chromosome 4; sequence tagged site.
SOURCE      Homo sapiens, clone p4-459 from a chromosome 4 plasmid library;
            vector pBluescript II KS + (Stratagene)
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 424)
  AUTHORS   Goold,R.D., diSibio,G., Dugaiczyk,A., Smith,K.A., Xu,H.,
            Magrane,G., Lang,D.B., Cox,D.R., Masters,S.B. and Myers,R.M.
  TITLE     Sequence-Tagged Sites for Human Chromosome 4
  JOURNAL   Unpublished (1992) see COMMENT for author address
  STANDARD  full automatic
COMMENT     PCR components: 25 ng of human genomic DNA, 10 pmol of each
            oligonucleotide, 200 micro-M dNTPs, 0.25 U Taq  polymerase (Cetus)
            in 10 micro-l of 50 mM KCl-20 mM  Tris-HCl, pH 8.3 (at room temp),
            2.5 mM MgCl-2.  Initial denaturation at 94degC for 1.5 min, then 30
            cycles of  94degC for 15 sec, 62degC for 23 sec, and 72degC for 30
            sec, followed by a final extension at 72degC for 3.5 min, using a
            Perkin-Elmer 9600 thermocycler. PCR-amplified product size 209 bp.
            STS sequence contains a simple sequence tandem repeat (CA).
            Sequence submitted by:
            Human Genome Mapping Center
            Box 0925
            University of California San Francisco
            San Francisco, CA 94143-0925  USA
            Phone: (415) 502-1612   Fax: (415) 476-8391
            e-mail: hgmcprobes@cgl.ucsf.edu
FEATURES             Location/Qualifiers
     misc_feature    22..44
                     /label=Primer1
     misc_feature    complement(207..230)
                     /label=Primer2
BASE COUNT      152 a     97 c     62 g    104 t      9 others
ORIGIN
        1 gatctcaaac acaaacccaa agcagactgc tgaaatggca tgtttagcaa caaaggacca
       61 ggcagacaaa aaaaatacac acacacacac acacacacac acacacacac attatttntt
      121 attgngggat aaaatatttc atcaaattaa gaagacaaaa tttagaactt gnggtcttca
      181 acacatgcat agcactaaac ctgtggctaa cattcattgc attccctcac gacaatttgt
      241 atttagtntg tgaacttggt ttagtccttc agccatgttc attaaatatt gactgctgtg
      301 tccttttacc atctgcaaag cacagatatc aangtaccat aatagagact tgnnaacaca
      361 cacacacaca atccgntggc cttatggaat gtacaaaaac ccaacatctt ctngggttac
      421 agag
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human chromosome 4 sequence-tagged site STS4-431
Date: 21 Aug 92 10:10:18 GMT
Distribution: bionet
Checksum: 11621     3

LOCUS       HUM4STS431    449 bp ds-DNA             PRI       21-AUG-1992
DEFINITION  Human chromosome 4 sequence-tagged site STS4-431
ACCESSION   L00857
KEYWORDS    STS; human chromosome 4; sequence tagged site.
SOURCE      Homo sapiens, clone p4-386 from a chromosome 4 plasmid library;
            vector pBluescript II KS + (Stratagene)
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 449)
  AUTHORS   Goold,R.D., diSibio,G., Dugaiczyk,A., Smith,K.A., Xu,H.,
            Magrane,G., Lang,D.B., Cox,D.R., Masters,S.B. and Myers,R.M.
  TITLE     Sequence-Tagged Sites for Human Chromosome 4
  JOURNAL   Unpublished (1992) see COMMENT for author address
  STANDARD  full automatic
COMMENT     PCR components: 25 ng of human genomic DNA, 10 pmol of each
            oligonucleotide, 200 micro-M dNTPs, 0.25 U Taq  polymerase (Cetus)
            in 10 micro-l of 50 mM KCl-20 mM  Tris-HCl, pH 8.3 (at room temp),
            2.5 mM MgCl-2.  Initial denaturation at 94degC for 1.5 min, then 30
            cycles of  94degC for 15 sec, 62degC for 23 sec, and 72degC for 30
            sec, followed by a final extension at 72degC for 3.5 min, using a
            Perkin-Elmer 9600 thermocycler. PCR-amplified product size 182 bp.
            Sequence submitted by:
            Human Genome Mapping Center
            Box 0925
            University of California San Francisco
            San Francisco, CA 94143-0925  USA
            Phone: (415) 502-1612   Fax: (415) 476-8391
            e-mail: hgmcprobes@cgl.ucsf.edu
FEATURES             Location/Qualifiers
     misc_feature    30..51
                     /label=Primer1
     misc_feature    complement(187..211)
                     /label=Primer2
BASE COUNT      126 a     91 c     80 g    139 t     13 others
ORIGIN
        1 gatcaatgca ctatgggccc tgcccccagg gggttctcag tcaaggatgg ggggtgatat
       61 atgtacatgg aaaataatat tcttgttgag aatggtaagt gtcatattac ataacaaatc
      121 taatgaaata gtatcaccag ctgccaaaat tttagaatca aatttgnaac cctctctctc
      181 acaaaagcac aggatattat ggcatatctt ttctgtacca acaccatttc tggtaccatc
      241 tcctnntctg acttttattt tccttntggc taaattttcc aattcatttt aagtctatta
      301 tctctagaaa tattgtctaa tctttgcaaa tcccctcttg aacaangang gttgnnaata
      361 aagggatatg tggntgtctt cacaaagcca cgatanatat gcacttgact gatgtatncc
      421 nccggntcgg ctcagagggc tcatgtgcc
//