From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Western diamondback rattlesnake preprometalloproteinase;
Date: 21 Jul 92 10:04:05 GMT
Distribution: bionet
Checksum: 48059     6

LOCUS       CRLPREHTE    1975 bp ss-mRNA            VRT       21-JUL-1992
DEFINITION  Western diamondback rattlesnake preprometalloproteinase;
            hemorrhagic toxin e; atrolysin e; mRNA, complete cds.
ACCESSION   M89784 J05372
KEYWORDS    atrolysin e; hemorrhagic toxin e; preprometalloproteinase; zymogen.
SOURCE      Crotalus atrox (library: H-lib) adult venom gland cDNA to mRNA.
  ORGANISM  Crotalus atrox
            Eukaryota; Animalia; Chordata; Vertebrata; Reptilia; Lepidosauria;
            Squamata; Serpentes; Culobroidea; Viperidae; Crotalinae.
REFERENCE   1  (bases 1 to 1975)
  AUTHORS   Hite,L.A., Shannon,J.D., Bjarnason,J.B. and Fox,J.W.
  TITLE     Sequence of a cDNA Clone Encoding the Zinc Metalloproteinase
            Hemorrhagic Toxin e From Crotalus atrox: Evidence for Signal,
            Zymogen and Disintegrin-like Structures
  JOURNAL   Biochemistry 31, 6203-6211 (1992)
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     5'UTR           1..61
                     /gene="Ht-e"
                     /note="putative"
                     /citation=[1]
                     /partial
     sig_peptide     62..115
                     /product="secretory protein"
                     /gene="Ht-e"
                     /note="putative"
                     /codon_start=1
                     /citation=[1]
     CDS             62..1498
                     /EC_number="3.4.24.44"
                     /note="precursor to hemorrhagic toxin e"
                     /product="preprometalloproteinase"
                     /gene="Ht-e"
                     /note="putative"
                     /codon_start=1
                     /translation="MIQVLLVTICLAAFPYQGSSIILESGNVNDYEVIYPRKVTALPK
                     GAVQPKYEDTMQYELKVNGEPVVLHLEKNKGLFSKDYSETHYSFDGRKITTNPSVEDH
                     CYYHGRIENDADSTASISACNGLKGHFKLQGEMYLIEPLKLSDSEAHAVFKLKNVEKE
                     DEAPKMCGVTQNWESYEPIKKASDLNLNPEHQRYVELFIVVDHGMYTKYNGDSDKIRQ
                     RVHQMVNIMKESYTYMYIDILLAGIEIWSNGDLINVQPASPNTLNSFGEWRETDLLKR
                     KSHDNAQLLTSIAFDEQIIGRAYIGGICDPKRSTGVVQDHSEINLRVAVTMTHELGHN
                     LGIHHDTDSCSCGGYSCIMSPVISDEPSKYFSDCSYIQCWEFIMNQKPQCILKKPLRT
                     DTVSTPVSGNELLEAGIECDCGSLENPCCYATTCKMRPGSQCAEGLCCDQCRFMKKGT
                     VCRVSMVDRNDDTCTGQSADCPRNGLYG"
                     /citation=[1]
     misc_feature    116..622
                     /note="propeptide"
                     /product="zymogen"
                     /gene="Ht-e"
                     /note="putative"
                     /citation=[1]
     mat_peptide     623..1228
                     /product="hemorrhagic toxin e; atrolysin e"
                     /gene="Ht-e"
                     /evidence=EXPERIMENTAL
                     /codon_start=1
                     /citation=[1]
     mat_peptide     1229..1495
                     /note="potential antiplatelet peptide"
                     /product="disintegrin-like peptide"
                     /gene="Ht-e"
                     /note="putative"
                     /codon_start=1
                     /citation=[1]
     3'UTR           1499..1975
                     /gene="Ht-e"
                     /note="putative"
                     /citation=[1]
     polyA_signal    1957..1962
                     /gene="Ht-e"
                     /note="putative"
                     /citation=[1]
     polyA_site      1975
                     /gene="Ht-e"
                     /note="putative"
                     /citation=[1]
BASE COUNT      608 a    406 c    407 g    554 t
ORIGIN
        1 ctcagattgg cttgaaagcg ggaagagatt gcctgtcttc cagccaaatc cagcctccaa
       61 aatgatccaa gttctcttgg tgactatatg cttagcagct tttccttatc aagggagctc
      121 tataatcctg gaatctggga acgtcaatga ttatgaagta atttatccac gaaaagtcac
      181 tgcattgccc aaaggagcag ttcagccaaa gtatgaagac accatgcaat atgaattgaa
      241 agtgaatgga gagccagtgg tccttcatct ggaaaaaaat aaaggacttt tttcaaaaga
      301 ttacagtgag actcattatt cctttgatgg cagaaaaatt acaacaaacc cttcagttga
      361 ggatcactgc tattatcatg gacgcatcga gaatgatgct gactcaactg caagcatcag
      421 tgcatgcaac ggtttgaaag gacatttcaa gcttcaaggg gagatgtacc ttattgaacc
      481 cttgaagctt tccgacagtg aagcccatgc agtcttcaaa ttgaaaaatg tagaaaaaga
      541 ggatgaggcc cccaaaatgt gtggggtaac ccagaattgg gaatcatatg agcccatcaa
      601 aaaggcctct gatttaaatc ttaatcctga acatcaaaga tatgttgagc ttttcattgt
      661 tgtggaccat ggaatgtaca caaaatataa tggcgattca gataagataa gacaacgggt
      721 acatcaaatg gtcaacatta tgaaggagag ttacacttat atgtacatcg atatattact
      781 ggctggtata gaaatttggt ccaacggaga tttgattaac gtgcagccag catcgcctaa
      841 tactttgaac tcatttggag aatggagaga gacagatttg ctgaagcgca aaagtcatga
      901 taatgctcag ttactcacgt ccattgcctt cgatgaacaa attataggac gggcttatat
      961 aggtggcata tgcgacccaa agcgttctac aggagttgtc caggatcata gcgaaataaa
     1021 tcttagggtt gcagttacaa tgacccatga gctgggtcat aatctgggca ttcatcatga
     1081 cacagattcc tgttcttgcg gtggttactc atgcattatg tctcctgtga taagcgatga
     1141 accttccaaa tatttcagcg attgtagtta catccaatgt tgggaattta ttatgaatca
     1201 gaagccacaa tgcattctca agaaaccctt gagaacagat actgtttcaa ctccagtttc
     1261 tggaaatgaa cttttggagg cgggaataga atgtgactgt ggctctcttg aaaatccgtg
     1321 ctgttatgct acaacctgta aaatgagacc agggtcacag tgtgcagaag gactgtgttg
     1381 tgaccagtgc agatttatga aaaaaggaac agtatgccgg gtatcaatgg ttgataggaa
     1441 tgatgatacc tgcactggcc aatctgctga ctgtcccaga aatggcctct atggctaaac
     1501 aacaatggag atggaaaggt ctgcagcaac aggcagtgtg ttgatgtgac tacagcctac
     1561 taatcaacct ctggcttctc tcagatttga tcttggagat ccttctttca gaaggttcaa
     1621 cttcccttta gtccaaagag acccacctgc ctgcatccta ctagtaaatc actcttacct
     1681 ttcatacgga atctaaattc tgcaatattt cttctccata tttaatctgt ttaccttttg
     1741 ctgtaatcaa accttttccc caccacaaag ctctatgggc atatacaaca ccaagggctt
     1801 atttgctgtc aagaaaaaca atggccattt taccgtttgc caattgcaaa gcacatttaa
     1861 tgcaacaagt tctgcctttt gagctggtat attcgaaatc aatgcttcct cttccaaaat
     1921 ttcatgctgc ctttccaaga tgtagctgct tccatcaata aactaactat tctca
//