From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: P.aeruginosa 6'-N-acetyltransferase (AAC (6')-II) gene, complete
Date: 29 Dec 92 19:59:09 GMT
Distribution: bionet
Checksum: 50713 4

LOCUS       PSEAAC6II    1507 bp ds-DNA             BCT       10-DEC-1992
DEFINITION  P.aeruginosa 6'-N-acetyltransferase (AAC (6')-II) gene, complete
            cds.
ACCESSION   M29695
KEYWORDS    acetyltransferase; gentamicin resistance; netilmicin resistance;
            tobramycin resistance.
SOURCE      P.aeruginosa (isolate SCH80090884) DNA, clones pSCH[884,1003].
  ORGANISM  Pseudomonas aeruginosa
            Prokaryotae; Gracilicutes; Scotobacteria; Pseudomonadaceae.
REFERENCE   1  (bases 1 to 1507)
  AUTHORS   Shaw,K.J., Cramer,C.A., Rizzo,M., Mierzwa,R., Gewain,K.,
            Miller,G.H. and Hare,R.S.
  TITLE     Isolation, characterization and DNA sequence analysis of an AAC
            (6')-II gene from Pseudomonas aeruginosa
  JOURNAL   Antimicrob. Agents Chemother. 33, 2052-2062 (1989)
  STANDARD  full automatic
COMMENT     Draft entry and computer-readable sequence for [1] kindly submitted
            by K.J.Shaw, 03-NOV-1989.
FEATURES             Location/Qualifiers
     -35_signal      430..436
                     /note="putative"
     -10_signal      458..463
                     /note="putative"
     RBS             534..539
     misc_feature    681..683
                     /note="Tn 21 recombinational hotspot"
     RBS             694..696
     RBS             749..751
     misc_feature    1256..1314
                     /note="integron '59 bp conserved box'"
     CDS             544..774
                     /note="ORF A"
                     /codon_start=1
                     /translation="MACYDCFFVQSMPRASKQQARYAVGRCLMLWSSNDVTQQGSRPK
                     TKLGSTERPFHVREHPPHNSSPHDRARPADAP"
     CDS             707..1261
                     /gene="AAC (6')-II"
                     /product="6'-N-acetyltransferase"
                     /codon_start=1
                     /translation="MSASTPPITLRLMTERDLPMLHDWLNRPHIVEWWGGDEERPTLD
                     EVLEHYLPRAMAEESVTPYIAMLGEEPIGYAQSYVALGSGDGWWEDETDPGVRGIDQS
                     LADPTQLNKGLGTRLVRALVELLFSDPTVTKIQTDPTPNNHRAIRCYEKAGFVREKII
                     TTPDGPAVYMVQTRQAFERKRGVA"
BASE COUNT      370 a    426 c    407 g    304 t
ORIGIN      
        1 ctcgggtaac atcaaggccc gatccttgga gcccttgccc tcccgcacga tgatcgtgcc
       61 gtgatcgaaa tccagatcct tgacccgcag ttgcaaaccc tcactgatcc gcatgcccgt
      121 tccatacaga agctgggcga acaaacgatg ctcgccttcc agaaaaccga ggatgcgaac
      181 cacttcatcc ggggtcagca ccaccggcaa gcgccgcgac ggccgaggtc ttccgatctc
      241 ctgaagccag ggcagatccg tgcacagcac cttgccgtag aagaacagca aggccgccaa
      301 tgcctgacga tgcgtggaga ccgaaacctt gcgctcgttc gccagccagg acagaaatgc
      361 ctcgacttcg ctgctgccca aggttgccgg gtgacgcaca ccgtggaaac gatgaaggca
      421 cgaacccagt ggacataagc ctgttcggtt cgtaagctat aatgcaagta gcgtatgcgc
      481 tcacgcaact ggtccagaac cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt
      541 ttcatggctt gttatgactg tttttttgta cagtctatgc ctcgggcatc caagcagcaa
      601 gcgcgttacg ccgtgggtcg atgtttgatg ttatggagca gcaacgatgt tacgcagcag
      661 ggcagtcgcc ctaaaacaaa gttaggcagc acagagcgcc catttcatgt ccgcgagcac
      721 cccccccata actcttcgcc tcatgaccga gcgcgacctg ccgatgctcc atgactggct
      781 caaccggccg cacatcgttg agtggtgggg tggcgacgaa gagcgaccga ctcttgatga
      841 agtgctggaa cactacctgc ccagagcgat ggcggaagag tccgtaacac cgtacatcgc
      901 aatgctgggc gaggaaccga tcggctatgc tcagtcgtac gtcgcgctcg gaagcggtga
      961 tggctggtgg gaagatgaaa ctgatccagg agtgcgagga atagaccagt ctctggctga
     1021 cccgacacag ttgaacaaag gcctaggaac aaggcttgtc cgcgctctcg ttgaactact
     1081 gttctcggac cccaccgtga cgaagattca gaccgacccg actccgaaca accatcgagc
     1141 catacgctgc tatgagaagg caggattcgt gcgggagaag atcatcacca cgcctgacgg
     1201 gccggcggtt tacatggttc aaacacgaca agccttcgag agaaagcgcg gtgttgccta
     1261 acaactcatt caagccgacg ccgcttcgcg gcgcggctta attcaggcgt tagatgcact
     1321 aagcacataa ttgctcacag ccaaactatc aggtcaagtc tgcttttatt atttttaagc
     1381 gtgcataata agccctacac aaattgggag atatatcatg aaaggctggc tttttcttgt
     1441 tatcgcaata gttggcgaag taatcgcaac atccgcatta aaatctagcg agggctttac
     1501 taagctt
//