From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: B.hermsii vmp7 gene encoding Vmp7 outer membrane lipoprotein
Date: 10 Apr 92 10:03:59 GMT
Distribution: bionet
Checksum: 06669    18

LOCUS       BORVMP7G     9118 bp ds-DNA             BCT       10-APR-1992
DEFINITION  B.hermsii vmp7 gene encoding Vmp7 outer membrane lipoprotein
ACCESSION   Z11876
KEYWORDS    Vmp7 outer membrane lipoprotein; insertion sequence;
            outer membrane lipoprotein.
SOURCE      Borrelia hermsii DNA.
  ORGANISM  Borrelia hermsii
            Prokaryota; Bacteria; Gracilicutes; Scotobacteria; Spirochetes;
            Spirochaetales; Spirochaetaceae.
REFERENCE   1  (bases 6807 to 8093)
  AUTHORS   Burman,N., Bergstroem,S., Restrepo,B.I. and Barbour,A.G.
  TITLE     The variable antigenes Vmp7 and Vmp21 of the relapsing fever
            bacterium Borrelia hermsii are structurally analogous to the VSG
            proteins ofthe African trypanosome
  JOURNAL   Mol. Microbiol. 4, 1715-1726 (1990)
  STANDARD  full staff_review
REFERENCE   2  (bases 8781 to 9118)
  AUTHORS   Kitten,T. and Barbour,A.G.
  TITLE     Juxtaposition of expressed variable antigen genes with a conserved
            telomere in the bacterium Borrelia hermsii
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 87, 6077-6081 (1990)
  STANDARD  full staff_review
REFERENCE   3  (bases 1 to 9118)
  AUTHORS   Barbour,A.G.
  JOURNAL   Unpublished (1991)
  STANDARD  full staff_review
REFERENCE   4  (bases 1 to 6876)
  AUTHORS   Barbour,A.G., Carter,C.J., Burman,N., Freitag,C.S., Garon,C.F. and
            Bergstrom,S.
  TITLE     Tandem insertion sequence-like elements define the expression site
            for variable antigen genes of Borrelia hermsii
  JOURNAL   Infect. Immun. 59, 390-397 (1991)
  STANDARD  full staff_review
REFERENCE   5  (bases 6689 to 6969)
  AUTHORS   Barbour,A.G., Burman,N., Carter,C.J., Kitten,T. and Bergstrom,S.
  TITLE     Variable antigen genes of the relapsing fever agent Borrelia
            hermsii are activated by promoter addition
  JOURNAL   Mol. Microbiol. 5, 489-493 (1991)
  STANDARD  full staff_review
REFERENCE   6  (bases 7901 to 8850)
  AUTHORS   Restrepo,B.I., Carter,C.J., Kitten,T. and Barbour,A.G.
  TITLE     Expression-linked telomeres of Borrelia hermsii linear plasmids are
            highly polymorphic
  JOURNAL   Unpublished (1992)
  STANDARD  full staff_review
REFERENCE   7  (bases 8094 to 8780)
  AUTHORS   Kitten,T., Burman,N., Bergstrom,S., Carter,C.J. and Barbour,A.G.
  TITLE     3' flanking region of active vmp7 gene of Borrelia hermsii.
            Sequence between end of gene and start of downstream homology
            sequence
  JOURNAL   Unpublished (1992)
  STANDARD  full staff_review
COMMENT     *source: strain=HS1 (ATCC 35209); *source: isolate=Serotype 7;
            *source: chromosome=Linear plasmid bp7E; *source: is_macronuclear=
            N; *source: is_proviral=N; *source: is_germline=N;
            From EMBL    entry BHVMP7G;  dated 27-MAR-1992.
FEATURES             Location/Qualifiers
     insertion_seq   1146..2121
                     /note="alternate"
                     /evidence=EXPERIMENTAL
                     /label=IS-like1
                     /citation=[2]
                     /citation=[1]
                     /citation=[5]
                     /citation=[6]
                     /citation=[3]
                     /citation=[4]
                     /citation=[7]
     insertion_seq   3347..4512
                     /note="alternate"
                     /evidence=EXPERIMENTAL
                     /label=IS-like2
                     /citation=[2]
                     /citation=[1]
                     /citation=[5]
                     /citation=[6]
                     /citation=[3]
                     /citation=[4]
                     /citation=[7]
     insertion_seq   5841..6789
                     /note="alternate"
                     /evidence=EXPERIMENTAL
                     /label=IS-like3
                     /citation=[2]
                     /citation=[1]
                     /citation=[5]
                     /citation=[6]
                     /citation=[3]
                     /citation=[4]
                     /citation=[7]
     -35_signal      6811..6815
                     /citation=[5]
                     /citation=[6]
                     /citation=[3]
     -10_signal      6833..6838
                     /citation=[5]
                     /citation=[6]
                     /citation=[3]
     mRNA            6846..>6846
                     /evidence=EXPERIMENTAL
                     /label=mRNA_start
                     /citation=[5]
                     /citation=[6]
                     /citation=[3]
     CDS             6874..7983
                     /product="Vmp7 outer membrane lipoprotein"
                     /gene="vmp7"
                     /evidence=EXPERIMENTAL
                     /label=expressed
                     /translation="MRKRISAIINKLNISIIIMTVVLMIGCGQQPEAGKTGVSGGVNG
                     NLGNSLMELGRSAENAFYAFIELVSDVLGFTAKSDTTKQEVGGYFNSLGAKLGEASND
                     LEQVAVKAETGVDKSDSSKNPIREAVNEAKEVLGTLKGYVESLGTIGDSNPVGYANNA
                     AGSGTTAADDELRKAFKALQEIVKAATDAGVKALKIGATTLQANGGADNKEGAKILAT
                     SGGNPAAADVAKAAAILSSVSGEEMLSSIVKSGENDAQLAAAADGNTSAISFAKGGSD
                     AHLAGANTPKAAAVAGGIALRSLVKTGKLAAGAADNATGGGKEVQGVGVAAANKLLRA
                     VEDVIKKTVKNVLEKAKEKIDKARGSQEPVSESSK"
                     /citation=[5]
                     /citation=[6]
                     /citation=[3]
     misc_recomb     8804..9017
                     /organism="Borrelia hermsii"
                     /note="DHS=Downstream Homology Sequence"
                     /evidence=EXPERIMENTAL
                     /label=DHS
                     /citation=[6]
                     /citation=[3]
     misc_feature    9096..9118
                     /note="Similar totelomeric sequences of linear plasmids of
                     Borreliaburgdorferi and poxviruses."
                     /label=Telomere
                     /citation=[2]
                     /citation=[5]
                     /citation=[6]
                     /citation=[3]
                     /citation=[7]
BASE COUNT     3210 a   1429 c   1851 g   2628 t
ORIGIN
        1 ctgcagaagc aacaattagt ggcccagtaa ataattagtt gagggtaaat actaaggaaa
       61 actctttttt ttctttctgt gaaacaggga gactatttgg ctagcggtag ttttgatgct
      121 gaaggtaatg cataagtaaa aggaggcacg taaaaaaatg agaagaataa attaagtgca
      181 ataataatga ctttatttat ggtattagta agctgtaata atggaggacc aaagcttaaa
      241 agtgacgaag tagccaagtc tgacggaaca gtacttgatt tggcaaaaat aagtaaaaaa
      301 ataaaagatg ctagtgattt tgcaacaagt gtaaaagaag ttcatacttt agttaagtca
      361 atagatgagc ttgctaaagc tattgggaaa aaaattcata acgatggttc tcttactact
      421 gaagatggta agaatggttc attacttgca ggggtacata gtgtaatatc agcctaaaga
      481 ctaaattggg atcattggaa caaaaagcta ttggagaatc tgctggaatg aaggttcaag
      541 ttgttgctat taagactgca agtatgattt attaaataaa tttaaagata aaaatgctga
      601 acttgggaaa acgaggttag taatgacgat gcgaaagctg ccatacttgt aagtaatacc
      661 actaaagata aaggagcttc tgagttgaag cactcaacac agcaatagat gggttgttaa
      721 aggctgctaa tggtgcagta gaagctgcaa tagcagagct tacaactcct gttaagggag
      781 aaaaaccttc tcaaaataac taactaggaa ataaataatt taagtagtta ttataagata
      841 agtatttaag taaaaagtaa actaaccctc ctgtatcaat aacaagaaaa gcgtttccct
      901 tacaataact ttatccttta tacttatcct taactagtta ttagtagtgc ctttaggggc
      961 gctctgttca ggagttatag gagtatcatt agcattaatt ttcatagctt ctttaaccgt
     1021 tttaagtccc acatcaattg tttttcttat tgcaatagtt aatgtatcta atgctttagt
     1081 tactgaactt attgctgctc ctttaattgc agtaacagca tcatcagcag cagcactagg
     1141 gccagcaaat ttaccaccct ttgccataac tcttaaagct atacctcctg caatagctcc
     1201 atctttaggg gcagcaccag cattttgagc agtagctaat ttagcagcat caccattatc
     1261 tttaatcata gcttgtaata tgtcagcacc agttacagtc ctactgcttt tgctgcatca
     1321 gctgcaactt tttttgcatt attagcatca ccagcagcac ctgcaccagc agtaaataat
     1381 ttacccgctt caccatcgcc agcagcagca gttcttgcag tattgccatc ttcagccttt
     1441 ttatcatcac cagcattagc acttcctaca cctttaagta ccacgtctac aattgtcttt
     1501 attcctttta ctagtttgtc aacttcagtc ccagcagcac cagcagcatt ctgagcagca
     1561 acattagcaa ttgggtcact agcatctcca atagcatcac taacggtctt agcaccttct
     1621 attatcttat caagagtatt atcaattagt gtttttaccg cagtctcagt tgcagaagca
     1681 ttaggatttc cttctctctt catgtcagta acaattttat taagcttgtc cttagtgcct
     1741 tgtatagtat cttgtattgt cttaaaataa gccccaacat cagacttttt tgtctctgta
     1801 ctaaaaccta ataccttgga aactatatct ccaaatgatg taaacacatt taaaaaatca
     1861 ttacctaagt ctattactga ctttaagaaa gcaaatatgc tgctggtgat gctaataatg
     1921 caaaaaaagt tgcagctgat gcagcaaaag cagttggagc aataacagat gctgatatat
     1981 tacaagctat ttctaaaggt tctgaatgca aagctgctaa attagctaag agtaatgatg
     2041 gcaatgttgg tgtttcccct aaagatggaa ctatagcagg agctattgca ttaacagtta
     2101 tggctaaggg tggtaaattt gctggtccta gtgatgctgc tagtgctgat gcaaagaaaa
     2161 taatagagag ctcagctact aaggcgctag atacattaac tattgcgata aggaaaacaa
     2221 ttgacgcagg ccttaaaaca attaaagaag ctatgaaaat taatcctaat gatactcctg
     2281 taactactga taatcagatt cctgaaacta agaagaacta atattagaag taataagtag
     2341 taaataacta aataaagtta tttaaggaga actcttctat tgttttcgat gcatgagagg
     2401 tatttcctta tttatatcta tctcctttac ttaaatagag taataaagga ggcacggaaa
     2461 aaaatgagaa gaagaataag tgcaataata atgactttat ttatggtatt tacatcatgt
     2521 aataatggag gaccaaagct taaaagtgac gaagtagcca agtctgacgg aacagtactt
     2581 gatttggcaa aaataagtgc aaagataaaa gaggctagta cttttgcagt aagtgttaaa
     2641 gaagttcata ctttagttaa atcagtagat gagcttgcta aggctattgg taagaaaatt
     2701 tagcaggaca ccgatacctt aggtactgat ggaaaccata atggatcttt agttgcaggt
     2761 gtatttcaag taatgttgac agtaaaagtt aaattggaaa cgttggtaaa attagatggg
     2821 atttctagtg aactgaaaac aaaagttgat gatactaagg gcaaagctga aacattatta
     2881 tctaaagtga aaacaaaaca tactgatctt ggcaaacaag atgctactga tgggaatgca
     2941 aaaaatgcta tagatgtatc agatggtgct aaggataaag gagttgctga acttattaaa
     3001 ttaaacacag caatagatga gttgttaaag gctgctaatg gtgcagtaga agctgcaatt
     3061 aaagagctta cagctcctgt taagggagaa aaccttctca aaataactaa ctaggaaata
     3121 aataagttaa ctagttatta gtagtacctt ttaggggcgc tctgtttagg acttatagga
     3181 gtatcattcg cattaatttt catagtatct ttaactgttt taagccctgt gtcaattgtt
     3241 tttcttatcg caatagttag tgtatctagt gccttagtaa cagcacttac agctgagctc
     3301 tctattattt tctttgcatc agcactagca gcatcactag gaccagcaaa tttaccaccc
     3361 ttagccattg ctcttaacac tatacctcct gctataacca catctttttt attagcatca
     3421 acagcagcaa cagcagcatt attagaagcc aatttcatag cctcaccatt atctttaacc
     3481 atagcttgca agatgtcagc acctgttaca gccccaatag cttttgctgc atcagtagcc
     3541 gatttttttg cattctcagc agtgccagca ttagcagaag caaataactt tcccgcttca
     3601 ccatcaccag cattgccacc gatccttgca gtattaccat cgctagcttt tttatcatcc
     3661 ccagccccag catttccttt gtctttaagt actatttcta caattgactt aattcctttt
     3721 actagtttat caacatcagt tccaacagta ccagcatcag caccagcacc accagcagca
     3781 acattagcaa ttggatcatt agcatcagta ccaatagcct cacttgctgt ctttgctccg
     3841 tcaattatct tatcaagtgt agtagtaatt aagtatttca atgatattga aaagattatg
     3901 aacacagtta aaaagaagtt gcaagatgaa gttgcaaaga atgataatta cgtaaaagtg
     3961 aaggaagtcg ttgacaagtt cgttgcagat gtcttagaca agattgcagt aggtgctaaa
     4021 gaagcagcta aaggggctac tggtgacgat aaaattggaa atgctacttc agctgggcat
     4081 ggagctattc ctgctagcaa ggattctgtt gtttttcttg ttaaagggat taagacactt
     4141 gttgaagtgg ttctaaaaag gatgaggggg ggatgcagga gctactaaaa caggtgatga
     4201 taaaaaggat attggtacat tgtttattga taataccggt aaagatgatg ctaaggaaga
     4261 aaatatttca aaggcatcag ctagtattgg tgctgtaact ggtgctgata ttcttcaagc
     4321 tattgctaaa tctgaagaaa atcctgttgt tgataatact aatggcatta acgcagccaa
     4381 aaatgcagct gaaattgctg ttgctccggc tgttaacggc aagaaagaag agttgccgta
     4441 gactcagcaa aaaaagatgc agtaatagca gcaggaatag tattaagagc aatagctaaa
     4501 gatggtaaat ttattgtaaa ggatactggt gacaataaga ctgaagctga agccgctaaa
     4561 ggtgtagcag caagtgcagt aggtaagaca ttaagtacac ttataatagc aataaggaat
     4621 acagttgata gtggattaaa aacaataaat gaagtactag ctaaagttaa acaagaagat
     4681 aagtcagcag aagcaactaa tactgcagaa gcaacaatta gtggcccagt aaataattag
     4741 ttgagggtaa atactaagga aaactctttt ttttctttct gtgaaacagg gagactattt
     4801 ggctagcggt agttttgatg ctgaaggtaa tgcataagta aaaggaggca cgtaaaaaaa
     4861 tgagaagaat aaattaagtg caataataat gactttattt atggtattag taagctgtaa
     4921 taatggagga ccaaagctta aaagtgacga agtagccaag tctgacggaa cagtacttga
     4981 tttggcaaaa ataagtaaaa aaataaaaga tgctagtgat tttgcaacaa gtgttaaaga
     5041 agttcatact ttagttaaat cagtagatga gcttgctaag gctattggta acgaaaattt
     5101 agcaggacac tgatacctta ggactgatgg aaaccataat ggatctttag ttgcaggtgt
     5161 atttcaagta atgttgacag taaaagttaa attggaaacg ttggtaaaat tagatgggat
     5221 ttctagtgaa ctgaaaacaa aagttgatga tactaagggc aaagctgaaa cattattatc
     5281 taaagtgaaa acaaaacata ctgatcttgg caaacaagat gctactgatg ggaatgcaaa
     5341 aaatgctata gatgtatcag atggtgctaa ggataaagga gttgctgaac ttattaaatt
     5401 aaacacagca atagatgagt tgttaaaggc tgctaatggc gcagtagaag ctgcaatagc
     5461 agagcttaca actcctgtta aggtagaaaa accttctcaa aataactaac taggaaataa
     5521 ataatttaag tagttattat aagataagta tttaagtaaa aagtaaacta accctcctgt
     5581 atcaataaca agaaaagcat ttccttacaa tgactttata tttaccctta actaattagc
     5641 agtagcttta ggggtgttct gttcaggact tataggagta tcattagcat taattttcat
     5701 agcttcttta actgttttaa gacctgcgtc aattgttttt cttattgcaa tagttaatgt
     5761 atctagtgct ttagtaattg cacttacagc tgctccttta gcaacagtga cataatcagc
     5821 actatcagca gtaggaccag caaatttacc acccttagcc ataactgtta atgcaatagc
     5881 tcctgctata gttccatctt ttgcattagc cttggcagca tcagcagctg cattagcacc
     5941 atttttaacc atagcttgta atatgtcagc accagttaca gccccaacgg cttttgctgc
     6001 atcggctgca actttttttg cattagcatc atcaccagca ttagcagcga acaatttacc
     6061 tgcttcacca ttaccagcag cggttcttgc gctaagatct tcagcctttt tatcggtacc
     6121 tgcctcagca tttccttctt ttccaagtac cacgtctaca attgccttaa ttccctttgt
     6181 cagactttca atcccagttc cagcaacacc agcagcattg ttagcaccta cattagcaat
     6241 tgggtcacta gcatctccaa tagcatcact aacggtcttt gatccttcta ttatcttatc
     6301 aagagtatta tcaattagtg tttttaccgc agtctcagtt gcagaagcat taggatttcc
     6361 ttctctcttc atgtcagtaa caattttatt aagcttgtcc ttagtgcctt gtatagtatc
     6421 ttgtattgtc ttaaaataag ccccaacatc agactttttt gtctctgtac taaaacctaa
     6481 taccttggaa actatatctc caaatgatgt aaacacattt aaaaaatcat tacctaagtc
     6541 tattactgac tttaagaaag caaatatgct gctggtgatg ctaataatgc aaaaaaagtt
     6601 gcagctgatg cagcaaaagc agttggagca ataacagatg ctgatatatt acaagctatt
     6661 tctaaaggtt ctgaatgcaa agctgctaaa ttagctaaga gtaatgatgg caatgttggt
     6721 gtttccccta aagatgaaac tatagcaaga gctatagcat taagagaatg gctaaaggtg
     6781 ataaatttga tttttttttt ttttttaact ttgtaaactt tgaaagttga ggtataatgc
     6841 taatgcataa gttaaaagga ggcacgtaaa aaaatgagaa aaagaataag tgcaataatt
     6901 aataagttaa atataagtat aattattatg acagttgttc taatgatagg ttgtggacaa
     6961 caaccagaag caggtaagac cggagtatca ggaggagtaa atggaaattt aggcaattca
     7021 ctaatggaat taggtaggag tgcggagaat gctttttacg catttataga gttagtgtca
     7081 gatgtgttgg gatttactgc aaaatcagat acaactaagc aagaagtagg aggttatttt
     7141 aacagcctag gtgcgaagct tggagaggcg tcaaatgact tggaacaagt agcagtaaaa
     7201 gcagaaacag gtgttgataa aagcgattca tcaaaaaatc caattagaga agcggttaat
     7261 gaagctaagg aagttttagg tacattaaaa ggatatgtag aatctttagg aacaataggc
     7321 gattctaatc cagtaggtta tgcaaataat gctgctggtt caggaacaac agcagctgat
     7381 gatgaattaa ggaaagcttt taaagcattg caagaaatag tcaaagcagc aacagatgca
     7441 ggtgttaaag cattaaaaat aggagctact acactacaag caaatggagg agcagataat
     7501 aaagagggtg ctaagatatt agctacaagt ggtggtaatc cagcagcagc agatgtagct
     7561 aaagcagcag caatactatc aagcgtaagt ggtgaagaga tgttaagctc aatagttaaa
     7621 tcaggagaga atgatgcgca gctagcagca gctgcagatg gaaatacaag tgcaatttct
     7681 tttgcaaaag gaggttcaga tgctcactta gcaggtgcaa atactccaaa agcagcagca
     7741 gtagcaggag gaatagcatt acgttcatta gtgaagacag gtaaattagc agcaggagca
     7801 gcagataatg ctacaggagg ggggaaagaa gtacaaggag taggagtggc tgcagcaaat
     7861 aagctgttaa gagcggtaga agatgtaatt aagaagacag taaagaatgt tcttgagaaa
     7921 gcaaaagaaa aaatagataa agcaagaggt tcacaagagc cagtttcaga atcaagtaag
     7981 tgagaaggca gattaataag ataaatagta aatttagatt attgaagaat aaaagcagaa
     8041 ggcaatctta gagagatgga tctaggttgc cttctacttt gttttaagta aaataagaag
     8101 ttaaatataa tagtggaaaa gaataagtgc aataataatg actttgttta tggtgtttat
     8161 gtcatgtaat aatggagggc cagagcttaa aagtgacgaa gtagctaagt ctgacggaac
     8221 agtacttgat ttggcaaaga taagtaaaaa gatcaaagag gctagtgctt ttgcagcaag
     8281 tgttaaagaa atagagactt tagttaagtc tattgatgag cttgctaaag ctattggaaa
     8341 aaagattaaa aatgatggaa ctttggaagc catagctgat aagaatggct cattaattgc
     8401 aggggtggtt agtgttgctg aagctgtgga aaaaaaatta ggagagttgc aagtagcagg
     8461 attccttaaa ggcttaaatg aaaaagttca agatgtggat gctaaggtta aagcatttac
     8521 aaagaagtta aaagataaac atgttgtttt gggagctgca gatggagcta ctacagatga
     8581 taatgcaaaa aaagctatag atcgcgtaaa tcaagttaat ggggagaatg gagcggaaga
     8641 acttggcaag ctcaacacag cagttgatgc cttgttaaag gcagctgagg gtgaagtaga
     8701 agctgcaatt acagagctta cagttcctgt taaggtagaa aaaccttctc aaaataacta
     8761 actagggaat aaataattta agtagttatt ataagataag ttaattaagt aagctgtaag
     8821 agcagggaaa aataaagtca aaaaggaata ggaagctagg agcgtaatgc tcttagcttc
     8881 taatgttatt tagggaatgt ttatttgtat ataaaattgt ttatatgagt aaagattgaa
     8941 tataaataat tgcaagtatg atattaagag tatgttttta ttgtaatcaa ataattaata
     9001 ctttaaaagt aagctaaatg tgtggtaagg gcagcaaaag ggaaattggg atagatgttg
     9061 gaaggaaaag aagcactggg gaagcggcaa ggggatttgt actaatatca aattatat
//