From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: S.typhimurium flagellar protein (fliT) gene, 3' end, alpha-amylase
Date: 5 Nov 92 23:13:40 GMT
Distribution: bionet
Checksum: 43855 5

LOCUS       STYFTAA      1700 bp ds-DNA             BCT       11-SEP-1992
DEFINITION  S.typhimurium flagellar protein (fliT) gene, 3' end, alpha-amylase
            (amyA) gene, complete cds.
ACCESSION   L01643
KEYWORDS    1,4-alpha-D-glucan glucanohydrolase; alpha-amylase; amyA gene;
            flagellar protein; fliT gene; glycogenase.
SOURCE      Salmonella typhimurium (strain SJW1103) DNA.
  ORGANISM  Salmonella typhimurium
            Prokaryotae; Gram-negative facultatively anaerobic rods;
            Enterobacteriaceae.
REFERENCE   1  (bases 1 to 1700)
  AUTHORS   Raha,M., Kawagishi,I., Mueller,V., Kihara,M. and Macnab,R.M.
  TITLE     Escherichia coli produces a cytoplasmic alpha-amylase, AmyA
  JOURNAL   J. Bacteriol. 174, 6644-6652 (1992)
  STANDARD  full automatic
REFERENCE   2  (sites)
  AUTHORS   Macnab,R.M., Irikura,V.M., Williams,A.W., Mueller,V. and
            Kawagishi,I.
  TITLE     Subdivision of flagellar region III of the Escherichia coli and
            Salmonella typhimurium chromosomes and identification of two
            additional genes
  JOURNAL   J. Gen. Microbiol. 138, 1051-1065 (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     CDS             <1..29
                     /number=1
                     /function="unknown"
                     /citation=1
                     /evidence="EXPERIMENTAL"
                     /citation=[2]
                     /citation=[1]
                     /gene="fliT"
                     /product="flagellar protein"
                     /codon_start=3
                     /translation="VPDAPGAS"
     CDS             101..1585
                     /number=2
                     /EC_number="3.2.1.1"
                     /note="This gene lies in the intervening region between
                     flagellar regions IIIa and IIIb"
                     /evidence="EXPERIMENTAL"
                     /citation=[2]
                     /citation=[1]
                     /gene="amyA"
                     /product="alpha-amylase"
                     /codon_start=1
                     /translation="MKNPTLLQYFHWYYPDGGKLWSELAERADGLNDIGINMVWLPPA
                     CKGASGGYSVGYDTYDLFDLGEFDQKGTIATKYGDKRQLLTAIDALKKNNIAVLLDVV
                     VNHKMGADEKERIRVQRVNQDDRTQIDDNIIECEGWTRYTFPARAGQYSNFIWDYHCF
                     SGIDHIENPDEDGIFKIVNDYTGDGWNDQVDDEMGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTHCDGFRLDAVKHIPAWFYKEWIEHVQAVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVDGKTMLFDAPLQMKFHEASRQGAEYDMRHIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGASYEDSGENGETCRVDMPVI
                     NQLDRLILARQRFAHGIQTLFFDHPNCIAFSRSGTEENPGCVVVLSNGDDGEKTLLLG
                     DNYANKTWRDFSGNRDEYVVTNDQGEATFFCNAGSVSVWVIEDV"
BASE COUNT      417 a    425 c    469 g    389 t
ORIGIN      
        1 tcgtgccaga tgcgcctggc gcctcataat attttcccgt ctcgtatgaa aattcttcca
       61 tactccagag gtcggctaaa cgacttctgg agcacggaag atgaaaaacc ccacgttatt
      121 gcagtacttc cactggtatt atcccgacgg cggtaaactc tggtctgagc tggcggaacg
      181 tgctgatggg ctgaatgata tcggtatcaa tatggtctgg ctaccgcccg cctgtaaagg
      241 cgcctccggc ggctattccg taggctatga tacctacgac ctgtttgacc tcggcgaatt
      301 tgaccaaaaa ggaactatcg cgacaaagta cggcgataaa cgccagttac tgacggcgat
      361 agacgcgctc aaaaaaaata atattgcggt gctgctcgac gtcgtcgtga accacaaaat
      421 gggcgcagac gaaaaagaac gtattcgcgt tcagcgcgtg aatcaggatg accgcacgca
      481 aatcgatgac aacatcattg aatgcgaagg ctggacgcgc tacaccttcc ctgcccgcgc
      541 gggccagtat tccaacttta tttgggacta tcactgtttc agcggcattg atcacatcga
      601 gaatcccgac gaagacggca tttttaagat cgtcaatgac tataccggcg atggctggaa
      661 cgatcaggtt gatgatgaga tgggtaattt cgactatctg atgggtgaaa atatcgattt
      721 tcgcaatcat gcggttacgg aagagattaa atattgggct cgttgggtca tggaacaaac
      781 ccactgtgac ggctttcgcc tggacgcggt aaaacatata cccgcctggt tttataaaga
      841 atggattgag catgtacagg cggttgcgcc aaaaccgctg tttattgtcg cagaatactg
      901 gtcgcatgaa gtggataaac tgcaaacgta catcgatcag gtcgacggga aaaccatgct
      961 gttcgacgcg ccgttgcaga tgaaatttca cgaggcctcg cgccagggcg cggagtatga
     1021 catgcgccac attttcaccg gcacgctggt agaagccgac ccttttcatg cggtgacgct
     1081 ggtcgctaac cacgatacac aaccgttaca ggcgctggaa gcgccggtag aaccctggtt
     1141 caaaccattg gcctatgcgc tgatcctgct tcgtgaaaac ggcgtgccgt cagtgtttta
     1201 tcccgattta tacggcgcca gctatgagga tagcggcgaa aatggcgaga cctgtcgggt
     1261 cgacatgccg gtgattaacc aactggatcg gctgatcctc gctcgtcagc gttttgcgca
     1321 cggtatacaa acactctttt tcgatcatcc taactgtatc gcctttagtc gcagcggtac
     1381 tgaagagaat ccaggctgtg tggtcgtact ttccaatggc gacgacggtg aaaaaaccct
     1441 cctgctcggc gacaattacg ctaacaagac ctggcgtgat ttttctggaa accgggatga
     1501 gtatgttgta actaatgatc aaggcgaagc gacgttcttc tgcaacgcag gcagcgtcag
     1561 cgtgtgggtc attgaggacg tgtgatgagc gttcggcgca gagcgctgcc gggaagcgaa
     1621 attacggcag cggcgtcggc agcggcgtcg gcagcggcgt tttttctaac gcggcgggcc
     1681 actccggcgt cggacgagcg
//