From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Yeast (FUN25 and FUN26) genes, partial cds; carbon catabolite
Date: 6 Nov 92 18:36:25 GMT
Distribution: bionet
Checksum: 41975 17

LOCUS       YSCF568CC    8700 bp ds-DNA             PLN       02-NOV-1992
DEFINITION  Yeast (FUN25 and FUN26) genes, partial cds; carbon catabolite
            repressor protein (CCR4) gene, partial cds; (FUN28) gene.
ACCESSION   L05027
KEYWORDS    repressor protein.
SOURCE      Saccharomyces cerevisiae (strain AB972) DNA.
  ORGANISM  Saccharomyces cerevisiae
            Eukaryota; Fungi; Eumycota; Hemiascomycetes; Endomycetales;
            Saccharomycetaceae.
REFERENCE   1  (bases 1 to 8700)
  AUTHORS   Clark,M.W., Zhong,W., Keng,T., Storms,R.K., Barton,A., Kaback,D.B.
            and Bussey,H.
  TITLE     Identification of a Saccharomyces cerevisiae Homolog of the SNF2
            Transcriptional Regulator in the DNA sequence of an 8.6kb Region in
            the LTE1-CYS1 Interval on the leftarm of Chromosome I
  JOURNAL   Yeast 8, 133-145 (1992)
  STANDARD  full automatic
REFERENCE   2  (bases 1 to 8700)
  AUTHORS   Ouellette,F., Clark,M.W., Keng,T., Storms,R.K., Zhong,W., Zeng,B.,
            Fortin,N., Delaney,S., Barton,A., Kaback,D.K. and Bussey,H.
  TITLE     Sequencing of Chromosome I from Saccharomyces cerevisiae: analysis
            of a 32 kilobase region between the LTE1 and SPO7 genes
  JOURNAL   Genome (1993) In press
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     misc_feature    complement(7474..8099)
                     /note="This feature applies to a gene which lacks a coding
                     region feature. "
                     /gene="FUN28"
     CDS             complement(7474..8099)
                     /note="ORF YAL020 ; homology to RCC1 from Golden Hamster
                     (Swiss-Prot #P23800), 6.9e-13 on a BLASTP score."
                     /note="RCC1 homologue"
                     /gene="FUN28"
                     /codon_start=1
     misc_feature    8099..8700
                     /note="overlap with accession number S93805, complete ORF
                     present for YAL020"
                     /citation=[1]
     CDS             complement(135..2409)
                     /function="unknown"
                     /partial
                     /note="ORF YAL23"
                     /gene="FUN25"
                     /codon_start=1
                     /translation="MSSSSSTGYSKNNAAHIKQENTLRQRESSSISVSEELSSADERD
                     AEDFSKEKPAAQSSLLRLESVVMPVIFTALALFTRMYKIGINNHVVWDEAHFGKFGSY
                     YLRHEFYHDVHPPLGKMLVGLSGYLAGYNGSWDFLLEIYPDYLDYVKMRLFNASFSAL
                     CVPLAYFTAKAIGFSLPTVWLMTVLVLFENSYSTLGRFILLDSMLLFFTVASFFSFVM
                     FHNQRSKPFSRKWWKWLLITGISLGCTISVKMVGLFIITMVGIYTVIDLWTFLADKSM
                     SWKTYINHWLARIFGLIIVPFCIFLLCFKIHFDLLSHSGTGDANMPSLFQARLVGSDV
                     GQGPRDIALGSSVVSIKNQALGGSLLHSHIQTYPDGSNQQQVTCYGYKDANNEWFFNR
                     ERGLPSWSENETDIEYLKPGTSYRLVHKSTGRNLHTHPVAAPVSKTQWEVSGYGDNVV
                     GDNKDNWVIEIMDQRGDEDPEKLHTLTTSFRIKNLEMGCYLAQTGNSLPEWGFRQQEV
                     VCMKNPFKRDKRTWWNIETHENERLPPRPEDFQYPKTNFLKDFIHLNLAMMATNNALV
                     PDPDKFDYLASSAWQWPTLNVGLRLCGWGDDNPKYFLLGTPASTWASSVAVLAFMATV
                     VILLIRWQRQYVDLRNPSNWNVFLMGGFYPLLAWGLHYMPFVIMSRVTYVHHYLPALY
                     FALIILAYCFDAGLQKWSRSKCGRIMRFVLYAGFMALVIGCFWYFSPISFGMEGPSSN
                     FRYLNWFSTWDIADKQEA"
     CDS             complement(2737..4288)
                     /function="unknown"
                     /partial
                     /note="ORF YAL022"
                     /gene="FUN26"
                     /codon_start=1
                     /translation="MSTSADTDTIKKPILAVPEPALADTHSEEISRSGEEHESENNEH
                     SDEEGDNYSEREQSVSTEPLDTLPLRKKLKNLSYITFFAIGIGLLWPWNCILSASQYF
                     KHDIFKDTSIWAKIFTSSMMSFSTISSMLFNIYLAKRQYKYSRRVINGLVWEIIVFTV
                     MCFFTILHFLLPKWFNFMFIMMLVVISSMGTAMTQNGIMAIANVFGSEYSQGVMVGQA
                     VAGVLPSLVLFALAFIENSSVSTTGGILLYFFTTTLVVTICVVMFSVSKISRKVNENW
                     NVEDGHITDVLLGSLRSNEEEIRIVGRIDQMEDEDHRRTNGTRDDNDEGEELQLKVPF
                     EVLFAKLKYLVLSIFTTFVVTLVFPVFASATYVTGLPLSNAQYIPLIFTLWNLGDLYG
                     RVIADWPMFRDQKFTPRKTFIYSLLRVAAIPLFLMFTAITSSSSGDEEHNGSVIVDLC
                     YMLLQFLFGVTNGHVISMSFMKVPEQLDNDDEKEAAGGFTNIFVSTGLALGSIISYVF
                     VFIIDFIIR"
     CDS             complement(4706..7217)
                     /function="carbon catabloite repressor protein"
                     /note="ORF YAL021 ; this gene is also known as FUN27"
                     /partial
                     /gene="CCR4"
                     /product="repressor protein"
                     /codon_start=1
                     /translation="MNDPSLLGYPNVGPQQQQQQQQQQHAGLLGKGTPNALQQQLHMN
                     QLTGIPPPGLMNNSDVHTSSNNNSRQLLDQLANGNANMLNMNMDNNNNNNNNNNNNNN
                     NGGGSGVMMNASTAAVNSIGMVPTVGTPVNINVNASNPLLHPHLDDPSLLNNPIWKLQ
                     LHLAAVSAQSLGQPNIYARQNAMKKYLATQQAQQAQQQAQQQAQQQVPGPFGPGPQAA
                     PPALQPTDFQQSHIAEASKSLVDCTKQALMEMADTLTDSKTAKKQQPTGDSTPSGTAT
                     NSAVSTPLTPKIELFANGKDEANQALLQHKKLSQYSIDEDDDIENRMVMPKDSKYDDQ
                     LWHALDLSNLQIFNISANIFKYDFLTRLYLNGNSLTELPAEIKNLSNLRVLDLSHNRL
                     TSLPAELGSCFQLKYFYFFDNMVTTLPWEFGNLCNLQFLGVEGNPLEKQFLKILTEKS
                     VTGLIFYLRDNRPEIPLPHERRFIEINTDGEPQREYDSLQQSTEHLATDLAKRTFTVL
                     SYNTLCQHYATPKMYRYTPSWALSWDYRRNKLKEQILSYDSDLLCLQEVESKTFEEYW
                     VPLLDKHGYTGIFHAKARAKTMHSKDSKKVDGCCIFFKRDQFKLITKDAMDFSGAWMK
                     HKKFQRTEDYLNRAMNKDNVALFLKLQHIPSGDTIWAVTTHLHWDPKFNDVKTFQVGV
                     LLDHLETLLKEETSHNFRQDIKKFPVLICGDFNSYINSAVYELINTGRVQIHQEGNGR
                     DFGYMSEKNFSHNLALKSSYNCIGELPFTNFTPSFTDVIDYIWFSTHALRVRGLLGEV
                     DPEYVSKFIGFPNDKFPSDHIPLLARFEFMKTNTGSKKV"
BASE COUNT     2264 a   1915 c   2012 g   2509 t
ORIGIN      
        1 gttaacaatt aaagagtgtt tatcgaaatt cattatatag tggtttatat agaccacttc
       61 ttctgctggt tgatatagaa attttattta attcttgttt tttacttatg tacttactac
      121 gatgatctta attcatgctt cttgcttgtc ggcaatgtcc caagtggaaa accagtttaa
      181 gtagcggaag ttactacttg gtccctccat accaaatgat attggggaga agtaccagaa
      241 gcaaccaatt acaagtgcca tgaatccggc gtataggacg aaccgcatga tacggccgca
      301 cttagatctg gaccattttt gcaaaccggc gtcgaaacag tacgccaaaa tgatcagtgc
      361 aaaatacaag gcaggcaagt aatgatgaac gtaggtgact ctagacatga taacgaatgg
      421 catgtagtgt aggccccaag ctagtagtgg gtagaacccg cccattaaga aaacgttcca
      481 gttagatgga tttcttaggt ccacatattg tctttgccat ctgatcagta agataacgac
      541 cgtggccatg aatgcgagga cggcaacact agaagcccac gtggaagctg gggtacccaa
      601 taggaagtat tttggattat catcacccca gccacatagt ctcaaaccca cattcaaagt
      661 tggccattgc catgctgagg aagctaagta atcaaatttg tctggatctg gcaccaaagc
      721 gttattagtg gccatcatgg ctagatttaa atgaatgaag tcttttaaga agttggtctt
      781 tgggtattga aaatcttcgg gtcttggtgg caacctttca ttttcgtggg tctcgatgtt
      841 ccaccaggtc ctcttgtccc tcttgaatgg gtttttcatg cagacaacct cttgttgtct
      901 gaaaccccat tcgggcaaac tgttaccggt ttgagccaag taacagccca tctccaagtt
      961 cttgatacgg aaagaggtgg tcaatgtgtg caacttctca gggtcttcat ctcctctttg
     1021 gtccatgatc tcaataaccc aattgtcttt gttgtcacca acaacattgt caccgtaacc
     1081 agaaacctcc cattgtgtct ttgacactgg tgcagcaact gggtgggtgt gcaagtttct
     1141 gcccgtgctt ttgtgtacca atctatagga ggtacctggc ttcaaatact cgatgtcagt
     1201 ttcgttttct gaccatgatg gtaagcctct ttctctgttg aaaaaccatt cgttgttagc
     1261 atctttgtaa ccataacagg ttacttgttg ttggttggac ccatctggat aagtttgtat
     1321 atgtgagtgc aatagagatc ctccaagagc ttggtttttg atggaaacaa cggaggaacc
     1381 tagagcaatg tcacgggggc cttgtccgac gtcagaaccc actaatcttg cttggaaaag
     1441 agatggcatg ttagcatcac ctgtaccaga atgcgataat aggtcaaaat gtattttgaa
     1501 gcacaatagg aaaatgcaga aggggacgat aataagacca aatattcttg ccaaccagtg
     1561 gttaatatag gttttccatg acatggattt atctgccaaa aaggtccata agtcaatcac
     1621 agtatagata ccgaccatag tgatgataaa tagacccacc attttgacgg aaatagtgca
     1681 acccaaagaa ataccagtga tcaacagcca tttccaccac tttctagaga acggcttgga
     1741 cctctggttg tggaacataa caaaactaaa gaacgatgcg acagtgaaga aaagtagcat
     1801 ggagtccaaa agaatgaacc tgcccaaagt actatacgag ttttcaaaca aaaccaacac
     1861 ggtcatcagc caaactgttg gtaaagaaaa tccaatagct ttggcagtga agtaggccaa
     1921 tggcacacag agcgcggaaa atgacgcgtt gaacagtctc attttaacat aatccaaata
     1981 gtctgggtaa atttccagaa ggaagtccca agaaccgttg tagcctgcca aataaccaga
     2041 caacccgacc agcatttttc ctaggggagg atggacatcg tggtaaaatt cgtgtctcaa
     2101 gtaataagaa ccaaatttac caaagtgcgc ctcatcccaa acaacatggt tgttgatgcc
     2161 gattttgtac atcctggtaa acaacgccaa tgcagtaaag atcaccggca ttacaacgga
     2221 ttccaggcgt aacagtgagc tttgtgcagc gggcttttcc ttcgagaaat cttccgcgtc
     2281 tctctcatca gcgctcgaaa gttcctcact gacgctgatg gaagacgatt ctctttgtct
     2341 cagtgtattc tcttgcttaa tgtgggcggc attgtttttg ctgtacccgg tagacgaaga
     2401 cgaggacatg attgctggac cacggttcga aacagaatga cagtagcgat gtggactaag
     2461 ccggttctcc ttggtaatgt tgttagtctc gagaaatgac ctttttttta ccctcaaaaa
     2521 gaatgcaaca ctattaataa acagtacacg aaacggatct ttccgtaagt tctttcgttc
     2581 ttttttttcg tattcttttc ttatgttttt ttttttcagg gcgacgtgtc caataatatg
     2641 tatgtttgtc gctatgtacg agatattatt gctaagtgac agtaaatcgt atcacgactg
     2701 taaatgatgg cgtttcggta tgtacagtat ctatctacct gataataaag tcaattatga
     2761 agacgaatac gtagcttatg atgctgccca gagctagtcc tgtagaaacg aaaatattag
     2821 tgaatccacc ggccgcttcc ttctcgtcgt cgttgtccag ttgctctggc accttcatga
     2881 aactcataga gatgacgtgc ccgttggtta cgccgaaaag gaactgcagt agcatgtaac
     2941 acaagtcaac gattacggag ccattgtgct cttcatcgcc gcttgaagag gaggtgatgg
     3001 ctgtgaacat caagaacagt ggtattgcgg ccacccgcaa caatgagtag atgaaggttt
     3061 tgcgtggcgt aaatttctgg tcacggaaca tgggccagtc ggcaatgact cttccgtaaa
     3121 ggtcgcctag gttccacagc gtgaatatga gaggtatgta ctgtgcgttg cttaaaggaa
     3181 gccctgtcac gtaggtggca gacgcaaata caggaaaaac aagagttacg acaaacgtgg
     3241 tgaatatgga aagaaccagg tactttagtt tggcaaataa gacctcgaaa ggcactttta
     3301 gttggagttc ctcaccttcg tcgttgtcgt cgcgagtgcc gttggtgcgg cggtggtctt
     3361 catcctccat ttggtcaatg cggccaacaa tacggatttc ctcctcattg gagcgcagag
     3421 accctaacaa cacatcagtg atatgtccgt cttccacatt ccaattctcg ttcactttcc
     3481 gactgatttt gctcacgctg aacatgacca cacaaatggt gaccacgagt gttgtggtaa
     3541 aaaagtatag aagaatcccg cccgtagtag acacagaaga gttctcgatg aaagctaggg
     3601 caaaaagcac tagggagggc aggacaccag caacggcttg ccccaccatg acaccttgac
     3661 tgtactcgga accgaagacg ttggctatgg ccatgatacc attctgtgtc atggctgtcc
     3721 ccatggaact gatcactaca agcatcatta taaacatgaa attgaaccat ttaggtaaaa
     3781 ggaaatgcaa aattgtaaag aagcacatga cagtaaagac aataatctcc cacacaagcc
     3841 cgtttatgac ccttctcgag tatttgtact gtcttttggc caagtagatg ttgaacagca
     3901 ttgacgatat ggtagaaaag gacatcatag agcttgtgaa gatctttgcc caaatggagg
     3961 tgtctttgaa aatatcgtgc ttaaaatatt gcgaggcact gaggatacag ttccacggcc
     4021 ataaaagacc tattcctatg gcgaaaaatg taatatatga aagatttttc aacttttttc
     4081 ttaacggcaa tgtatccagt ggttcggttg acacagattg ctctctttca gaataattat
     4141 cgccttcttc atctgagtgc tcgttgttct ctgattcatg ttcttctcca gagcgtgata
     4201 tctcctctga atgcgtatcg gccagtgcag gctctggcac cgcaaggatt ggcttcttga
     4261 tggtatcagt gtccgcacta gtactcattt ttgcaatctg gtatgctttc ttgcgtttga
     4321 tgataacgtg tcgccgtaac gtaaatcatt catcctttcc tatgattttt taagtcaatt
     4381 tttttttctt tttcgggaaa ttaattctgt ttttggcatt aataacaaca agaagaatta
     4441 aggattcgtt atagaataca agattcttgg tttggttaaa taattttggc tctcttaaat
     4501 attataaaaa gatacttaag taagaaagaa acaggacgaa aaagatacgc aaaagagaga
     4561 gagagagaga gagaaaaaaa agatgagaaa aaaaagtata acgtgaaggc tttatgggaa
     4621 gtgcggtgag attgggatcg tttcaatttt ataatgaggt agtgtacaga gaggagggag
     4681 ggagtgggat gaaagtgtgc ggtttatact ttcttactgc ctgtgtttgt cttcataaat
     4741 tcaaatcttg ctaacaatgg tatatggtca ctggggaatt tgtcgttggg gaacccgata
     4801 aacttactca cgtattcagg gtccacttca cccaatagcc cacgcaccct tagagcatgt
     4861 gtagaaaacc atatatagtc gataacatct gtgaatgacg gtgtgaaatt ggtgaatggt
     4921 agttctccga tacaattata acttgatttg agagccaagt tatgtgagaa atttttctcc
     4981 gacatgtaac cgaaatctct accatttccc tcttgatgta tttggacacg gcctgtattt
     5041 atcaattcgt atacggcgga gttgatgtat gaattgaagt caccacaaat gagcacagga
     5101 aatttcttaa tgtcctgtct aaaattgtgc gatgtctcct cctttagcag cgtttccaga
     5161 tgatctaaca ggacacctac ttggaatgtc ttgacatcat taaattttgg atcccagtgc
     5221 aaatgcgtgg tgaccgccca tatggtgtcg ccactaggaa tgtgttgtag ctttaagaac
     5281 agtgcaacgt tgtctttgtt cattgcacgg tttaaataat cttcagttct ttggaacttc
     5341 ttgtgtttca tccaagcacc gctgaaatcc atggcgtctt tggtgatcaa cttgaattgg
     5401 tcccttttga aaaaaatgca acacccgtcc actttcttgg agtccttgga atgcatggtc
     5461 ttggctcttg cctttgcatg gaagatgcct gtataaccgt gcttgtccaa taggggcacc
     5521 caatactctt caaaagtctt agactccact tcttgtaaac acaacagatc actgtcgtac
     5581 gagagaatct gctcctttaa tttattgcgc ctgtaatccc aacttaacgc ccacgacggt
     5641 gtgtaacggt acatttttgg ggtggcatag tgttgacata aggtgttgta ggataagacg
     5701 gtgaacgtcc tcttggctaa atcggtggcc agatgctcag tggattgctg caaagaatcg
     5761 tactccctct gtggttcccc atcggtattg atttcgatga acctacgttc atgcggtaag
     5821 ggaatctctg gtctgttgtc ccttaggtag aaaatcaatc ccgtgacaga tttttctgta
     5881 aggatcttta aaaactgttt ttctaagggg tttccttcta caccaagaaa ctgaaggtta
     5941 cacaggttcc caaactccca tggtaatgtg gtgaccatgt tatcaaaaaa gtagaagtat
     6001 ttcaattgga aacatgagcc tagttccgct ggtagagatg ttaacctatt atgcgacagg
     6061 tccaaaacgc gtaggttgct taggttcttg atctccgctg gcagttccgt gaggctattg
     6121 ccattcaaat atagtctcgt tagaaaatcg tacttgaaga tgttggcgct gatattgaag
     6181 atttgcaagt tggacaaatc tagcgcgtgc cataattggt cgtcgtattt cgagtccttg
     6241 ggcatgacca ttctgttttc aatgtcgtca tcttcgtcga tgctgtactg agacagtttc
     6301 ttgtgttgta agagcgcctg gttggcttcg tccttgccat tagcaaacag ctctatcttg
     6361 ggagtcaatg gtgtagaaac tgcactgtta gttgccgtgc ctgagggagt gctatctccc
     6421 gtaggttgtt gtttctttgc tgtcttgctg tcggtgagag tgtcggccat ttccatcaag
     6481 gcttgctttg tgcagtctac cagtgatttg gaggcttctg caatgtgaga ttgctggaaa
     6541 tcggtgggct gcaaagctgg tggtgcagcc tgaggtccgg ggccaaatgg gcccgggacc
     6601 tgctgctgtg cctgttgctg cgcttgttgc tgagcttgct gagcctgttg tgtagccaaa
     6661 tacttcttca tagcgttttg tctagcataa atgttgggtt gccccagaga ttgtgcggac
     6721 actgcagcca gatgcaattg aagcttccag atcggattgt ttagtaaaga agggtcatcc
     6781 aagtgcgggt gtaataaagg gttactggcg ttcacattga tgttcacagg cgtaccaacg
     6841 gtaggaacca tccctataga atttactgcg gctgtggagg cattcatcat caccccgctt
     6901 ccaccgccgt tgttgttgtt gttgttgttg ttgttattgt tgttattgtt gttatccatg
     6961 ttcatattga gcatgttcgc gttcccatta gccaattggt cgagcaactg gcgcgagttg
     7021 ttgttgctgg aagtgtgtac atcgctgttg ttcatgagtc ccggtggagg tatcccggtg
     7081 agctggttca tgtgtagctg ctgctgtaga gcgtttggtg taccctttcc tagtagcccg
     7141 gcatgctgct gttgctgctg ttgctgttgc tgctgcgggc ccacattagg gtagcctagt
     7201 aaagaagggt cgttcattag cggtagtttg cagggataac gtcagtcgga gttcccttgc
     7261 tggtgtccct tatgctgtgc ccttgtgctt gagaaccttc tctaaggtgg aaactttttc
     7321 aaattttttc gttgttggcc cgtttttcgc aagtacgggc gataacaaaa ggcctaaacg
     7381 gctactttac ctttgagatc taccacgatg agaattaatg ctgaatggac atctatgtat
     7441 atgatagtgg gtatatagtt acttatcagt gctagagcac gatccacgtg gtggcacatc
     7501 cgccaaacac gcgaggtttt ccagagtatt ggcccacgag ctgcagtcca ggctgggacg
     7561 ccttttgcgg gccgcagttg ccatgctctc cccagcccca gcagtataca ttgtaacagt
     7621 gagacttgcc ttcttgatta gcagtagtta gaataccgtg ctcactcccg gttgcaacac
     7681 cgacaatagg gaagtctagg cgctcttgcg ggaagagttg ggaatgtgtg cccctaccaa
     7741 acgactctac cgtattgagg cgcgcattcc acaggtggat cgaggtccac atgcacagta
     7801 ccactagatt gtgtcttttt tgctgttgtt tgagctcgaa cccagtgggc aggcgaccgg
     7861 atgcgtgcac tatgcggccg ccctcgtcca ctatgaccat gaagtccttg cccatggcca
     7921 cgtagtctac ggccacagac ccggtatcgt acaccaatac gggctctttc agtgatcggg
     7981 atttgggctc ttgcaattga cactttgtgt tgctgcccca gccgtatact cgggtgcctt
     8041 gcaccaccac aaagttctgg aaacatccgt atactgcgat gcgctcatcg ttggaattca
     8101 atggcacatg ttgctgagtg aactcgtagc aaccgcctcc tctctgccat acacggccat
     8161 cagcatccac aataactgtc gtgtcccagc cgcacgccac atccaccacg ggtgccggta
     8221 cttccacggg cctccagtca tgcacctgcc gcagtgcttg cgcactatcc agttctcccc
     8281 gtctgttatc tccacatcct accagattcc cgtcatttgt cagcatcacg ctgtggttcc
     8341 caccgcacgc tatcttcctg actattgctc catcatctcc tggcacagac ctctgtgggg
     8401 tatccatatc ctcatcgtgc cccagtccca gttgcctttg cccattagac ccaaacgcat
     8461 acacacaact catcgataca agcctgttat agcctttaat gatcacattc catcacttgc
     8521 gctttggatc tgcctgcatt atcaaggctc aaacggctgc gttacccccg tcgccgcgaa
     8581 atttttcata atttttcact ttgtaggatt aaaagagatc atgagcccat ctcgcaatgc
     8641 aacacgtaac ttaaatcagt actggcgtgt gctatagtgc tctatattcg agtttgttgc
//