From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Bacillus thurigiensis 50MDa plasmid replication-associated protein
Date: 31 Dec 92 01:43:08 GMT
Distribution: bionet
Checksum: 51522 5

LOCUS       BACREP       2393 bp ds-DNA             BCT       18-DEC-1992
DEFINITION  Bacillus thurigiensis 50MDa plasmid replication-associated protein
            (rep) complete cds.
ACCESSION   M77344
KEYWORDS    rep gene; replication-associated protein.
SOURCE      Bacillus thuringiensis (strain HD73, sub_species kurstaki) DNA.
  ORGANISM  Bacillus thuringiensis
            Prokaryotae; Firmicutes; Endospore-forming rods and cocci;
            Bacillaceae.
REFERENCE   1  (bases 1 to 2393)
  AUTHORS   Gamel,P.H. and Piot,J.-C.
  TITLE     Characterization and properties of a novel plasmid vector for
            Bacillus thuringiensis displaying compatibility with host plasmids
  JOURNAL   Gene 120, 17-26 (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     rep_origin      123..1843
     RBS             437..451
                     /gene="rep"
     insertion_seq   complement(1822..2393)
                     /note="'3'-end of IR2150'; 'exhibits complete homology to
                     B.thuringiensis IS232 nt1612-end'"
     CDS             449..1387
                     /gene="rep"
                     /note="putative"
                     /product="replication-associated protein"
                     /codon_start=1
                     /translation="MLLKNFILSKAKRELSPQLDRISTKPGNINKAVKKILKEIDIIL
                     LEDQGFTTEDISLQTPRKYKGLETILSYLVHEGFSCVKQDVIAQKAGISKPLINETLS
                     WLEKLGICHQIRNRKAGKKGPSVYILTIHNNYQKIIDYFKHKWHLAIEITETISNLIS
                     KWTLLKEKKEEVTDEQVDTDPKQEEANEQSEEPKDYFARKEDFNEYLSKDQQRAWHYI
                     MSSPFTNLSEKDAYAIANRMPPDIDRDAWYFFRQAADRFEASKADKSNAAYFIEIFSQ
                     NYKSYLKRKKEEAEKYVSSLSKPKQKLIIYDFIKGE"
BASE COUNT      916 a    376 c    345 g    756 t
ORIGIN      
        1 agatcttagc attatataat ttgtactcta ttaaatcaaa aggtagaaat ccccttattt
       61 cccttaataa gatatataga aaaggtaatt caatccagat taatcaaaca gaagaatcaa
      121 gcctagtcac taggttgata tttcataaaa aaaaagatag aatatataac ataatatata
      181 taattaaaaa agagatttat gatatagtta tttcatataa acaggaaatt aagttcaatt
      241 catttaaaaa agacaaaaaa taaaactcca cccaccgttt tcaattgatt cgaagtttgg
      301 tgactagatc aattgaaaac ccacgttagc agagtttttt tacaaaatta atattatggt
      361 taaattatat cataactaat gaagttttgt gaataaaaaa ctcgctaatt ttccgatatc
      421 gggctggaaa tattagggag gttttttcgt gttattaaaa aattttatat taagcaaagc
      481 aaaacgagaa ctaagtcctc aactggatcg tatatctact aaaccaggaa atattaataa
      541 agccgtaaaa aaaattctaa aggagattga tatcattctt ttagaagacc agggctttac
      601 tactgaagac atttctttac aaactcctcg taagtacaaa ggacttgaaa caattttatc
      661 ttatcttgtt cacgaaggtt tttcttgtgt gaaacaggat gtgatagccc aaaaagcggg
      721 aatttcaaaa ccccttatca atgaaactct ttcttggcta gaaaaacttg gtatctgcca
      781 ccaaattaga aatcgtaaag ctggaaagaa aggtccttct gtatacattt taacaataca
      841 taataattat caaaaaatca tagattactt taaacacaaa tggcatctag caattgagat
      901 aacagaaact atctcaaacc taatatccaa gtggactcta ttaaaagaaa aaaaagaaga
      961 agttacagac gaacaagtag atacagatcc aaaacaagaa gaagctaatg aacaaagtga
     1021 agaacctaaa gactattttg ctagaaaaga agatttcaat gaatatttaa gtaaagatca
     1081 acaaagagca tggcactata ttatgagttc tccatttact aatctgtctg aaaaagatgc
     1141 atatgcaatt gcaaatagaa tgccaccaga tatcgacaga gatgcttggt atttcttcag
     1201 acaagctgcc gatcgttttg aagctagtaa agctgataag agtaatgctg cttatttcat
     1261 tgaaatattt agccaaaact ataaatctta tttaaaacgt aaaaaagaag aagctgaaaa
     1321 atatgtttca tctttatcaa aaccaaaaca aaagttaata atttacgatt ttatcaaagg
     1381 ggaataatct tgcttaaaat cgccattttt atagccttgt ttaaaaatta tagcaaaaca
     1441 aggctatttg tcatgcgctt atattatttc ctaaatacct ctcatacttc acatccaaat
     1501 caattaaaaa taacagttaa tttttagcat ataatggaaa aacataaaaa gataacctac
     1561 taaaattgcc gcaataaatg aaccgaaaat tcagatacct cctttgtttc taccaaataa
     1621 ctactaagaa aaaaaatcaa acttcttgtt agagtaaggg ttaagtgcca tttttaattt
     1681 ctaatgtatt taatggtttt aatttttaaa acaagattta ttaaataaaa gaatcttatc
     1741 ataattcatc aaattttttt aaacaccttt tgtcattctc cctactgttt ccctaatcct
     1801 cgctaaaata gacacccaat atgtaaatgt caagataaac atgtacattt tcgcttgttt
     1861 aagcatgtac aaaatcaatc attttctttg ctaaaatgat ctttaattcg ataggattgt
     1921 cctacaatac tgaccaccgt ggcatgatgt aagacacgat ctagtatggc attggcgagt
     1981 ttagggtcct ggaatacttc gtcccaagac ttgaagttga tattggtcgt taggatggta
     2041 ctacgctttt cataacgcat atcgattaat tgaaagaata attttgcatc ctccggatca
     2101 ataggcaagt acccaatttc atcaataata agtaatttgt attttgtata gtgctttaaa
     2161 cgagattcta ggcgattctc aatcttggca cgttttaaat tttgaagtaa atcatgacat
     2221 ttaataaaat aagtacttgt tcgcttttta gctgctgcta taccaataga cgtggccaaa
     2281 tgggtcttac caacaccact aggtcctaaa aatactatgt tttcttgttg ctctaagaaa
     2341 cgtagagaaa taaaatctaa gatttgttgt ttattaatac tcggctggaa ttc
//