HDTenEightyP/NetNews huggingface dataset:
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human ADP-ribosylation factor 1 gene, exon 1.
Date: 22 Aug 92 10:00:44 GMT
Distribution: bionet
Checksum: 09342     3

LOCUS       HUMADPRF1    1009 bp ds-DNA             PRI       22-AUG-1992
DEFINITION  Human ADP-ribosylation factor 1 gene, exon 1.
ACCESSION   M84327
KEYWORDS    ADP-ribosylation factor 1.
SEGMENT     1 of 2
SOURCE      Homo sapiens DNA.
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 1009)
  AUTHORS   Kahn,R.A., Kern,F.G., Clark,J., Gelmann,E.P. and Rulka,C.
  TITLE     Human ADP-ribosylation factors: A functionally conserved family of
            GTP-binding proteins
  JOURNAL   J. Biol. Chem. 266, 2606-2614 (1991)
  STANDARD  full staff_entry
REFERENCE   2  (bases 1 to 1009)
  AUTHORS   Lee,C.-M., Haun,R.S., Tsai,S.-C., Moss,J. and Vaughan,M.
  TITLE     Characterization of the human gene encoding ADP-ribosylation factor
            1, a guanine nucleotide-binding activator of cholera toxin
  JOURNAL   J. Biol. Chem. 267, 9028-9034 (1992)
  STANDARD  full staff_entry
FEATURES             Location/Qualifiers
     GC_signal       164..169
     GC_signal       600..605
     GC_signal       701..706
     GC_signal       complement(720..725)
     GC_signal       773..778
     GC_signal       complement(793..798)
     exon            858..903
                     /number=1
     conflict        865
                     /note="g in conflict"
                     /citation=[1]
     conflict        867
                     /note="g in conflict"
                     /citation=[1]
     intron          904..>1009
                     /number=1
BASE COUNT      151 a    375 c    353 g    130 t
ORIGIN
        1 aagaagacct gagggtggtg ggcagagcca ggcctccagc acacggaaca cagagggact
       61 gcaacggtcc tctgttcagg tgcctgagag cgggatctcc tccgcctcaa ctggatctca
      121 gcttaaaggc cagctcatta gagaccactg tgacctccct tctgggcggc cgtccccaac
      181 cctgggcccc acccccacgg cccccgcctc accccggggt ccccaggcca cctcctcctt
      241 tgcttcatct gcggccagct ccccggcgtc tgctccgggc ccaggtatga gcccgcgcgt
      301 cactgcggcc gccggggcgc cgaggccagt gcggggacac agcaagcctc ccgccttcgg
      361 accttccgcc gtggccccgc gagtgccaca ggtttccaca gggccggggc acgtggaccc
      421 gcgcagcctg ggagccagga accccaaccc cggcgagggg cgcctcgcac ccccgtcctc
      481 acgcagtccc cgaggcctcc cggtgactgc cgggtgtggg aggaggaagg tggcaaccag
      541 ccccgcagct ccagttgctg caggagcccc ccgggcctca gtctccaaat acgccaaacg
      601 ggcgggagtg agccacaggc tgtcttcgcg gaagactcac aggcgcaggc gcagacgggt
      661 gcgcgcccct agggtgcgcg cgagctaaga ccgcggcccg gggcggggtg tccccggccc
      721 cgccccctgc agccctaccg gagccagcgc gcctgcgtcg cgggcgcacg gtgggcggag
      781 ttcccggcgt ctccgcccct tacccggcgt gccccgcgcc cggaggcgct gacgtggccg
      841 ccgtcagagc cgccatcttg tgggagcaaa accaacgcct ggctcggagc agcagcctct
      901 gaggtgaggg cgaggggcgc gggccggtgt gggccgcaga gacgttggag ccggcggggg
      961 ctggggactg gcctcggggc gacttgaggt tcgcgaaggg cacgtcgac
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human chromosome 4 sequence-tagged site STS4-201
Date: 21 Aug 92 10:07:43 GMT
Distribution: bionet
Checksum: 47428     3

LOCUS       HUM4STS201    460 bp ds-DNA             PRI       21-AUG-1992
DEFINITION  Human chromosome 4 sequence-tagged site STS4-201
ACCESSION   L00905
KEYWORDS    STS; human chromosome 4; sequence tagged site.
SOURCE      Homo sapiens, clone C4-129 from Los Alamos National Laboratory
            chromosome 4 cosmid library, 1G3; vector SuperCos 1 (Stratagene)
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 460)
  AUTHORS   Goold,R.D., diSibio,G., Dugaiczyk,A., Smith,K.A., Xu,H.,
            Magrane,G., Lang,D.B., Cox,D.R., Masters,S.B. and Myers,R.M.
  TITLE     Sequence-Tagged Sites for Human Chromosome 4
  JOURNAL   Unpublished (1992) see COMMENT for author address
  STANDARD  full automatic
COMMENT     PCR components: 25 ng of human genomic DNA, 10 pmol of each
            oligonucleotide, 200 micro-M dNTPs, 0.25 U Taq  polymerase (Cetus)
            in 10 micro-l of 50 mM KCl-20 mM  Tris-HCl, pH 8.3 (at room temp),
            2.5 mM MgCl-2.  Initial denaturation at 94degC for 1.5 min, then 30
            cycles of  94degC for 15 sec, 62degC for 23 sec, and 72degC for 30
            sec, followed by a final extension at 72degC for 3.5 min, using a
            Perkin-Elmer 9600 thermocycler. PCR-amplified product size 244 bp.
            Sequence submitted by:
            Human Genome Mapping Center
            Box 0925
            University of California San Francisco
            San Francisco, CA 94143-0925  USA
            Phone: (415) 502-1612   Fax: (415) 476-8391
            e-mail: hgmcprobes@cgl.ucsf.edu
FEATURES             Location/Qualifiers
     misc_feature    32..56
                     /label=Primer1
     misc_feature    complement(252..275)
                     /label=Primer2
BASE COUNT      143 a     88 c     82 g    139 t      8 others
ORIGIN
        1 ggcaccncca nacttgaacg tctggtgagg gaccattcac tggttcatag atataggaac
       61 tgtgccctca cgtggtagaa ggggcaaagg agctctctgg aatctccttc atagggacac
      121 taatcccatt cttgaagttg ccacccacat gacctcatct aatcataatt acttctgaaa
      181 aaccctacct tgtaacacca tcacataaaa gtagggtttc aacatacgaa tttgctgggg
      241 gacacacaca ttcagtcaat agcagccatt ttgtctaaat ataaaaaaga taaaagaaag
      301 ttttgatttt tttgttgttg ttgttagaaa atgtggtctc tctattcatc ttaagtactt
      361 gaaaatttgc tctcagcaac attggaagtt taangtaaga ataancatgg ntatatctnc
      421 atttaaaaaa atnacacttg ccatattggg gnttttttta
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human immunodeficiency virus type 2 (73ENVD2) envelope fragment.
Date: 18 Aug 92 10:01:20 GMT
Distribution: bionet
Checksum: 44463     2

LOCUS       HIV7ENVD2     452 bp ss-RNA             VRL       18-AUG-1992
DEFINITION  Human immunodeficiency virus type 2 (73ENVD2) envelope fragment.
ACCESSION   M87140
KEYWORDS    envelope protein.
SOURCE      Human immunodeficiency virus type 2 uncultured blood mononuclear
            cells (9/90 sample) RNA.
  ORGANISM  Human immunodeficiency virus type 2
            Viridae; ss-RNA enveloped viruses; Positive strand RNA virus;
            Retroviridae; Lentivirinae.
REFERENCE   1  (bases 1 to 452)
  AUTHORS   Gao,F., Yue,L., White,A.T., Pappas,P.G., Barchue,J., Hanson,A.P.,
            Greene,B.M., Sharp,P.M., Shaw,G.M. and Hahn,B.H.
  TITLE     Human infection by genetically-diverse SIVsm-related HIV-2 in West
            Africa
  JOURNAL   Nature (1992) In press
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     misc_feature    1..452
                     /note="PCR derived HIV-2 envelope fragment"
                     /note="putative"
                     /citation=[1]
BASE COUNT      162 a     75 c    108 g    107 t
ORIGIN
        1 gacgtggtca agagacaaca agaatgttgc gactgaccgt ctggggaaca aaaaatctcc
       61 aggcaagagt cactgctatt gagaaatact taaaggacca ggcgcaacta aattcatggg
      121 gatgcgcgtt taggcaaatc tgccacacta ctgtaccatg ggtaaatgac agcttgacac
      181 ctgattggga caacatgacg tggcaacaat gggaaaaaca aatccgctac ctggaggcaa
      241 atatcagtga aagtctagaa caggcacaaa tccagcaaga aaagaacatg tatgaattac
      301 aaaaattaaa tagctgggat gtttttggca actggtttga tttagcctcc tgggtcaagt
      361 atattcagta tggagtttat atagtagtag gaatagtagc tcttagaata ataatatatg
      421 tagtacaaat gataggtaga cttagaagag gt
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: B.subtilis synthetic gene for histone-like HBsu protein
Date: 15 Aug 92 10:01:11 GMT
Distribution: bionet
Checksum: 60350     2

LOCUS       BACHBSUHOM    309 bp ds-DNA             BCT       15-AUG-1992
DEFINITION  B.subtilis synthetic gene for histone-like HBsu protein
ACCESSION   X66448
KEYWORDS    DNA binding protein; histone homologue; synthetic gene.
SOURCE      Bacillus subtilis DNA.
  ORGANISM  Bacillus subtilis
            Prokaryotae; Firmicutes; Endospore-forming rods and cocci;
            Bacillaceae.
REFERENCE   1  (bases 1 to 309)
  AUTHORS   Groch,N., Schindelin,H., Schollz,A.S., Hahn,U. and Heinemann,U.
  TITLE     Determination of DNA-binding parameters.....
  JOURNAL   Eur. J. Biochem. (1992) In press
  STANDARD  full automatic
COMMENT     From EMBL    entry BSHBSUHOM;  dated 06-AUG-1992.
FEATURES             Location/Qualifiers
     CDS             4..282
                     /gene="hbsu"
                     /codon_start=1
                     /translation="MNKTELINAVAEASELSKKDATKAVDSVFDTILDALKNGDKIQL
                     IGFGNFEVRERSARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVAGK"
BASE COUNT       99 a     71 c     75 g     64 t
ORIGIN
        1 catatgaaca aaacggagct cattaacgcc gttgccgaag ccagcgaact gagcaaaaaa
       61 gatgccacga aagccgttga tagcgttttt gatacgattc tagatgccct gaaaaacggc
      121 gataaaattc agctgattgg ctttggcaac tttgaagttc gtgaaagatc tgcccgtaaa
      181 ggccgtaacc cgcagacggg cgaagaaatt gaaattccgg ccagcaaggt accggccttt
      241 aaaccgggca aagctttaaa agatgccgtt gccggcaaat aataactgca gaattccaag
      301 cttggatcc
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human chromosome 4 sequence-tagged site STS4-336
Date: 21 Aug 92 10:09:06 GMT
Distribution: bionet
Checksum: 09950     3

LOCUS       HUM4STS336    419 bp ds-DNA             PRI       21-AUG-1992
DEFINITION  Human chromosome 4 sequence-tagged site STS4-336
ACCESSION   L00801
KEYWORDS    STS; human chromosome 4; sequence tagged site.
SOURCE      Homo sapiens, clone p4-281 from a chromosome 4 plasmid library;
            vector pBluescript II KS + (Stratagene)
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 419)
  AUTHORS   Goold,R.D., diSibio,G., Dugaiczyk,A., Smith,K.A., Xu,H.,
            Magrane,G., Lang,D.B., Cox,D.R., Masters,S.B. and Myers,R.M.
  TITLE     Sequence-Tagged Sites for Human Chromosome 4
  JOURNAL   Unpublished (1992) see COMMENT for author address
  STANDARD  full automatic
COMMENT     PCR components: 25 ng of human genomic DNA, 10 pmol of each
            oligonucleotide, 200 micro-M dNTPs, 0.25 U Taq  polymerase (Cetus)
            in 10 micro-l of 50 mM KCl-20 mM  Tris-HCl, pH 8.3 (at room temp),
            2.5 mM MgCl-2.  Initial denaturation at 94degC for 1.5 min, then 30
            cycles of  94degC for 15 sec, 62degC for 23 sec, and 72degC for 30
            sec, followed by a final extension at 72degC for 3.5 min, using a
            Perkin-Elmer 9600 thermocycler. PCR-amplified product size 206 bp.
            STS sequence contains a simple sequence tandem repeat (CA).
            Sequence submitted by:
            Human Genome Mapping Center
            Box 0925
            University of California San Francisco
            San Francisco, CA 94143-0925  USA
            Phone: (415) 502-1612   Fax: (415) 476-8391
            e-mail: hgmcprobes@cgl.ucsf.edu
FEATURES             Location/Qualifiers
     misc_feature    72..96
                     /label=Primer1
     misc_feature    complement(254..277)
                     /label=Primer2
BASE COUNT      134 a     85 c     76 g    119 t      5 others
ORIGIN
        1 atcaaaagna tttgaaacaa ataaacatat gaggcttttc tcttctcatg ccttcacctt
       61 ttcctactac tctggaagga agtattaatg tagctgataa ctcttaaatt tgacttctct
      121 atgaaacaaa gaaaagaaaa agtaatcata tatgaaaggt tggcaatgat gttcatgaaa
      181 aaaatttctg ttatggattt ttttcgtnta cacacacaca cacacacaca cacacacaca
      241 cacacacaan cacactgata tggtttggtt gtgtccccac ccaaacaaat ctcatcttga
      301 attgtagctc ccataattcc catgtgttat gagagggacc tggtgggaga taactgaatc
      361 atggggggat gttctcccat gcttgttctc gngatagtga gggggggnct cacaagatc
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human chromosome 4 sequence-tagged site STS4-250
Date: 21 Aug 92 10:08:13 GMT
Distribution: bionet
Checksum: 15812     3

LOCUS       HUM4STS250    449 bp ds-DNA             PRI       21-AUG-1992
DEFINITION  Human chromosome 4 sequence-tagged site STS4-250
ACCESSION   L00764
KEYWORDS    STS; human chromosome 4; sequence tagged site.
SOURCE      Homo sapiens, clone p4-194 from a chromosome 4 plasmid library;
            vector pBluescript II KS + (Stratagene)
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 449)
  AUTHORS   Goold,R.D., diSibio,G., Dugaiczyk,A., Smith,K.A., Xu,H.,
            Magrane,G., Lang,D.B., Cox,D.R., Masters,S.B. and Myers,R.M.
  TITLE     Sequence-Tagged Sites for Human Chromosome 4
  JOURNAL   Unpublished (1992) see COMMENT for author address
  STANDARD  full automatic
COMMENT     PCR components: 25 ng of human genomic DNA, 10 pmol of each
            oligonucleotide, 200 micro-M dNTPs, 0.25 U Taq  polymerase (Cetus)
            in 10 micro-l of 50 mM KCl-20 mM  Tris-HCl, pH 8.3 (at room temp),
            2.5 mM MgCl-2.  Initial denaturation at 94degC for 1.5 min, then 30
            cycles of  94degC for 15 sec, 62degC for 23 sec, and 72degC for 30
            sec, followed by a final extension at 72degC for 3.5 min, using a
            Perkin-Elmer 9600 thermocycler. PCR-amplified product size 203 bp.
            Sequence submitted by:
            Human Genome Mapping Center
            Box 0925
            University of California San Francisco
            San Francisco, CA 94143-0925  USA
            Phone: (415) 502-1612   Fax: (415) 476-8391
            e-mail: hgmcprobes@cgl.ucsf.edu
FEATURES             Location/Qualifiers
     misc_feature    76..100
                     /label=Primer1
     misc_feature    complement(255..278)
                     /label=Primer2
BASE COUNT      137 a     83 c     79 g    145 t      5 others
ORIGIN
        1 gatcagatat ctgntgaagg aacataagtc attttcattg catttttttc ctctataaaa
       61 ttatgacatc ctccccctgc tttcttataa cctttcaatg agtatttgtc agaataagac
      121 tcttctgaag aaaatgcaaa ccttacttat gatgataaaa gtttgagaca ggataaacaa
      181 aactcctagg aagaaaaatg attcactcat ttttnngnat tactcaccaa gggctgagtt
      241 gtgctggttt ccccctcagc attattcctc tgtgtcaatg gtttagtcca aaacacacta
      301 cagagtatgc actagagagc ctattaaaaa ctgctgcagc acccagaagt gtgttgagaa
      361 tgaattattg atgaaattct accggagatt tggcacgact atcacttatt cctatttggg
      421 gttatagaac attttttggn attgtgtga
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: B.oleracea gene for storage protein (pBo2SC)
Date: 15 Aug 92 10:01:14 GMT
Distribution: bionet
Checksum: 27972     2

LOCUS       BNA2SCI       537 bp ds-DNA             PLN       15-AUG-1992
DEFINITION  B.oleracea gene for storage protein (pBo2SC)
ACCESSION   X65970
KEYWORDS    2S storage protein; storage protein.
SOURCE      Brassica oleracea DNA.
  ORGANISM  Brassica oleracea
            Eukaryota; Plantae; Embryobionta; Magnoliophyta; Magnoliopsida;
            Dilleniidae; Capparales; Brassicaceae.
REFERENCE   1  (bases 1 to 537)
  AUTHORS   Dasgupta,J., Dasgupta,S. and Mandal,R.K.
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
COMMENT     *source: clone=pBo2SC;
            From EMBL    entry BO2SCG;  dated 06-AUG-1992.
FEATURES             Location/Qualifiers
     CDS             1..537
                     /product="2S storage protein"
                     /codon_start=1
                     /translation="MANKLFLVSATLAFFFLLTNASIYRTVVEFDEDDATNPAGPFRI
                     PKCRKEFQQAQHLRACQQWLHKQAMQSGGGPSWTLDSEFDFEDDMENPQGPQQRPPLL
                     LQCCNELDQEEPLCVCPTLKGASKAVKQQIQQQGQQQGKQQMVSRIYQTATHLPKVCN
                     IPQVSVCPFQKTMPGPSY"
BASE COUNT      146 a    160 c    125 g    106 t
ORIGIN
        1 atggcgaaca agctcttcct cgtctcggca actctcgcct tcttcttcct tctcaccaat
       61 gcctccatct accggacggt ggtcgagttc gacgaagatg atgccacaaa cccagccggc
      121 ccatttagga tcccaaaatg taggaaggag tttcagcaag cacaacacct aagagcttgc
      181 cagcagtggc tccacaagca agcaatgcag tctggcggtg gtcctagctg gaccctcgac
      241 agtgagtttg actttgaaga cgacatggag aacccgcagg gtccacagca gagaccgcct
      301 ctactcctgc aatgctgtaa cgagctggac caggaagagc ccctttgcgt ttgcccaacc
      361 ttgaaaggag catccaaagc ggttaaacaa caaattcaac aacagggaca acagcaagga
      421 aagcagcaaa tggtgagccg tatctaccag accgctacgc acttacctaa agtttgcaac
      481 atcccgcaag ttagcgtttg tcccttccag aagaccatgc ctgggccctc ctactag
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Lentinus velutinus ribosomal RNA (37LR3) large subunit, partial.
Date: 19 Aug 92 10:02:13 GMT
Distribution: bionet
Checksum: 03296     2

LOCUS       LENRRLSM      234 bp ss-rRNA            RNA       19-AUG-1992
DEFINITION  Lentinus velutinus ribosomal RNA (37LR3) large subunit, partial.
ACCESSION   M98598
KEYWORDS    large subunit ribosomal RNA.
SOURCE      Lentinus velutinus (library: Duke culture collection 795) cDNA to
            rRNA.
  ORGANISM  Lentinus velutinus
            Eukaryota; Fungi; Eumycota; Basidiomycotina; Hymenomycetes;
            Agaricales; Tricholomataceae.
REFERENCE   1  (bases 1 to 234)
  AUTHORS   Hibbett,D.S. and Vilgalys,R.
  TITLE     Phylogenetic relationships of the basidiomycete genus Lentinus
            inferred from molecular and morphological characters
  JOURNAL   Unpublished (1992)
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     rRNA            <1..>234
                     /note="Aligns to region between positions 371 to 597
                     inSaccharomyces cerevisiae large subunit nuclear rRNA."
                     /product="large subunit ribosomal RNA"
                     /citation=[1]
BASE COUNT       60 a     49 c     70 g     55 t
ORIGIN
        1 aagatgaaaa gcactttgga aagagagtta aacagtacgt gaaattgctg aagggaaacg
       61 cttgaagtca gtcgcgtcat ccagaactca gcctgcttct gcctggtgta ctttctggtt
      121 gacgggccag catcagtttt gaccgctgga aaaaggccat gggaatgtgg caccttcggg
      181 tgtgttatag cctgtggtca tatacagcgg ttgggactga ggaccgcagc acgc
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: H.sapiens dystrophin gene, exon 68.
Date: 20 Aug 92 10:03:03 GMT
Distribution: bionet
Checksum: 48816     2

LOCUS       HUMDYST09     354 bp ds-DNA             PRI       20-AUG-1992
DEFINITION  H.sapiens dystrophin gene, exon 68.
ACCESSION   M86892
KEYWORDS    Becker muscular dystrophy; Duchenne muscular dystrophy; dystrophin;
            integral membrane glycoprotein; membrane-associated protein.
SOURCE      Homo sapiens DNA.
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 354)
  AUTHORS   Roberts,R.G., Coffey,A.J., Bobrow,M. and Bentley,D.R.
  TITLE     Determination of the exon structure of the distal protion of the
            dystrophin gene by vectorette PCR
  JOURNAL   Genomics 13, 942-950 (1992)
  STANDARD  simple staff_entry
FEATURES             Location/Qualifiers
     exon            91..257
                     /number=68
                     /gene="dystrophin"
BASE COUNT       82 a     94 c     64 g    114 t
ORIGIN      chromosome X, map position Xp21
        1 ttaatcgaac tgatatacac ctcctttgcc atcttgcctt ctttcctttc atcctttggc
       61 cctccttctc tctccctcct gtctttgcag gctaataata agccagagat cgaagcggcc
      121 ctcttcctag actggatgag actggaaccc cagtccatgg tgtggctgcc cgtcctgcac
      181 agagtggctg ctgcagaaac tgccaagcat caggccaaat gtaacatctg caaagagtgt
      241 ccaatcattg gattcaggta ttaggaacca aaaaaaaaaa tgtcattttt ttctcatcat
      301 ttttcacctt tttatgactt gatcttttat ctcctgtgcc agttgctgtt agtt
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human chromosome 4 sequence-tagged site STS4-444
Date: 21 Aug 92 10:10:30 GMT
Distribution: bionet
Checksum: 22262     3

LOCUS       HUM4STS444    414 bp ds-DNA             PRI       21-AUG-1992
DEFINITION  Human chromosome 4 sequence-tagged site STS4-444
ACCESSION   L00867
KEYWORDS    STS; human chromosome 4; sequence tagged site.
SOURCE      Homo sapiens, clone p4-431 from a chromosome 4 plasmid library;
            vector pBluescript II KS + (Stratagene)
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 414)
  AUTHORS   Goold,R.D., diSibio,G., Dugaiczyk,A., Smith,K.A., Xu,H.,
            Magrane,G., Lang,D.B., Cox,D.R., Masters,S.B. and Myers,R.M.
  TITLE     Sequence-Tagged Sites for Human Chromosome 4
  JOURNAL   Unpublished (1992) see COMMENT for author address
  STANDARD  full automatic
COMMENT     PCR components: 25 ng of human genomic DNA, 10 pmol of each
            oligonucleotide, 200 micro-M dNTPs, 0.25 U Taq  polymerase (Cetus)
            in 10 micro-l of 50 mM KCl-20 mM  Tris-HCl, pH 8.3 (at room temp),
            2.5 mM MgCl-2.  Initial denaturation at 94degC for 1.5 min, then 30
            cycles of  94degC for 15 sec, 62degC for 23 sec, and 72degC for 30
            sec, followed by a final extension at 72degC for 3.5 min, using a
            Perkin-Elmer 9600 thermocycler. PCR-amplified product size 150 bp.
            Sequence submitted by:
            Human Genome Mapping Center
            Box 0925
            University of California San Francisco
            San Francisco, CA 94143-0925  USA
            Phone: (415) 502-1612   Fax: (415) 476-8391
            e-mail: hgmcprobes@cgl.ucsf.edu
FEATURES             Location/Qualifiers
     misc_feature    43..66
                     /label=Primer1
     misc_feature    complement(169..192)
                     /label=Primer2
BASE COUNT      102 a     77 c     82 g    141 t     12 others
ORIGIN
        1 gatcccacct acccatttac agttaacctc cactcccacc aggcaactac taatctactt
       61 tccgtcttta tagatttncc ttttctggat acttcgtaca aatatattca tagaaaatgg
      121 ggccttttgt gtctagtttt tgntgtntct tttttactta gcatttgtga cccattgatg
      181 tagtagcaca tgtgagtaat tcagtctttt attgctaaat agtatccctt ctatgagttt
      241 ggcacgtgtt taaccantca gcagttgacg gagatttggg tgttctcact tnttggntat
      301 tnngtgtgca agtattnggg tggcaaagag atgtatntnn cccccctgaa agtgaggcag
      361 cagtgaagga aatactatga gtcaagactg gagatgaaaa ctgtgtcatg ttta
//