From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: S.cerevisiae A364A of DBF4 gene encoding cdc7 protein kinase
Date: 15 Aug 92 10:03:36 GMT
Distribution: bionet
Checksum: 11327     6

LOCUS       YSCDBF4      2760 bp ds-DNA             PLN       15-AUG-1992
DEFINITION  S.cerevisiae A364A of DBF4 gene encoding cdc7 protein kinase
            subunit
ACCESSION   X60279
KEYWORDS    cdc7 protein kinase; protein kinase.
SOURCE      Saccharomyces cerevisiae DNA.
  ORGANISM  Saccharomyces cerevisiae
            Eukaryota; Fungi; Eumycota; Hemiascomycetes; Endomycetales;
            Saccharomycetaceae.
REFERENCE   1  (bases 1 to 2760)
  AUTHORS   Kitada,K., Johnston,L.H., Sugino,T. and Sugino,A.
  TITLE     Temperature-sensitive cdc7 mutations of Saccharomyces cerevisiae
            are suppressed by the DBF4 gene, which is required for the G1/S
            cell cycle transition.
  JOURNAL   Unpublished (1991)
  STANDARD  full automatic
REFERENCE   2  (bases 1 to 2760)
  AUTHORS   Sugino,A.
  JOURNAL   Unpublished (1991)
  STANDARD  full automatic
COMMENT     *source: strain=A364A; *source: haplotype=haploid; *source:
            clone_library=YEp213 genomic; *source: clone=pDBF4-1; *source:
            chromosome=VII;
            From EMBL    entry SCDBF4;  dated 12-SEP-1991.
FEATURES             Location/Qualifiers
     enhancer        121..126
     mRNA            277..>2448
     CDS             361..2448
                     /product="cdc7 protein kinase subunit"
                     /gene="DBF4"
                     /codon_start=1
                     /translation="MVSPTKMIIRSPLKETDTNLKHNNGIAASTTAAGHLNVFSNDNN
                     CNNNNTTESFPKKRSLERLELQQQQHLHEKKRARIERARSIEGAVQVSKGTGLKNVEP
                     RVTPKELLEWQTNWKKIMKRDSRIYFDITDDVEMNTYNKSKMDKRRDLLKRGFLTLNT
                     QITQFFDTTVTIVITRRRSVENIYLLKDTDILSRAKKKYMKVWSYEKAARFLKNLDVD
                     LDHLSKTKSASLAAPTLSNLLHNEKLYGPTDRDPRTKRGDIHYFKYPHVYLYDLWQTW
                     APIITLEWKPQELTNLDELPYPILKIGSFGRCPFIGDRNYDESSYKRVVKRYSRDKAN
                     KKYALQLRALFQYHADTLLNTSSVNDQTKNLIFIPHTCNDSTKSFKKWMQEKAKNFEK
                     TELKKTDDSAVQDVRNEHADQTDETKEPPLKEEKENKRSIAEESNKYPQRKELAATPK
                     LNHPVLATFARQETEEVPDDLCTLKTKSRQAFEIKASGAHQSNDVATSFGNGLGPTRA
                     SVMSKNMKSLSRLMVDRKLGVKQTNGNNKNYTATIATTAETSKENRHRLDFNALKKDE
                     APSKETGKDSAVHLETNRKPQNFPKVATKSVSADSKVHNDIKITTTESPTASKKSTST
                     NVTLHFNAQTAQTAQPVKKETVKNSGYCENCRVKYESLEQHIVSEKHLSFAENDLNFE
                     AIDSLIENLRFQI"
BASE COUNT     1014 a    519 c    499 g    728 t
ORIGIN
        1 tatctctttg catctaattc ctcattacct tttttatttt tattttctta tcggttcttc
       61 gaacgcctaa gtttctttta atattttacc cgcagtaagt aaatggtttt ttttttgcaa
      121 acgcgtctta ggtcgatctt tgcgaaaaag aaaaaaataa agaaataacg ataataataa
      181 cggtaatggc tcaggccaag aaaaacaaaa atcaagttgc atagtctata ctcagccagg
      241 tgtagattcc tttagatctg aattcgcttg cagggctagt tatcttcaga attcaaggtt
      301 tgaatctgaa aggcatttgg aattctgctt ttggttcata tttagaaaag aaagaagaaa
      361 atggtttctc caacgaaaat gataataagg tctccgttaa aggaaacaga taccaacttg
      421 aaacataata atggaattgc cgcatcaaca acagcagcgg gccacttgaa tgtgttttct
      481 aacgacaata attgcaacaa taacaatacc actgaatcct ttccgaagaa aagatctctt
      541 gagcgcctcg agctccaaca gcagcagcat ttgcatgaaa agaaaagggc cagaatagaa
      601 agggccaggt ctattgaagg cgctgtccaa gtcagcaaag gtacgggtct gaaaaatgtc
      661 gagccaaggg ttacgcctaa ggaattgctg gaatggcaaa caaattggaa aaagataatg
      721 aaaagagatt ctcgcattta ctttgacatt actgatgatg tagagatgaa tacatataat
      781 aagtccaaga tggacaaacg cagagattta ttgaaaagag ggtttcttac attaaataca
      841 caaataactc aattttttga cactactgtc acaatagtta tcacaagaag aaggtctgtt
      901 gagaacatat atttactaaa agataccgac attttatcga gagctaaaaa aaagtacatg
      961 aaagtttgga gttacgaaaa ggctgccaga tttctgaaaa atcttgatgt tgatttggat
     1021 catttgagca agactaaatc tgcttcttta gctgcgccca cattgtccaa tcttctacac
     1081 aatgaaaaat tatatggacc aacggataga gaccccagaa ctaaaagagg cgatattcac
     1141 tactttaaat atcctcatgt atacctttat gacctatggc aaacttgggc ccccataata
     1201 actttggaat ggaaacctca agaactaaca aacttagacg aactacctta cccaatattg
     1261 aaaataggtt cattcggaag atgccctttt ataggggata ggaattatga cgaaagttct
     1321 tataagcgcg tagtaaagag atactcgaga gacaaagcaa acaaaaaata tgcactgcaa
     1381 cttcgtgctc tatttcaata tcatgccgac accttactga atacgtcatc agttaatgat
     1441 caaacgaaaa acctaatatt catacctcac acatgcaacg attctaccaa gagcttcaaa
     1501 aaatggatgc aagaaaaggc aaaaaatttt gagaagaccg agttaaagaa gacggatgat
     1561 agcgcagttc aagatgttcg taatgaacat gctgaccaaa ccgatgaaac aaaagagcct
     1621 ccgttgaaag aagaaaaaga aaataagcga tctatagcag aagaatcgaa taagtaccca
     1681 cagcgaaaag agctggctgc cacaccaaaa ctaaaccatc cagtattagc tacttttgca
     1741 aggcaagaaa ctgaagaagt gccggatgat ttgtgcactt tgaaaacaaa gtcacgtcag
     1801 gcatttgaaa tcaaagcaag tggtgcacat caatctaatg atgtggcaac ctcttttggc
     1861 aatggtttgg gcccaacaag agcaagcgtc atgagtaaga acatgaagtc attaagtaga
     1921 ctaatggttg atagaaagct gggagtaaag cagacaaatg gaaataacaa aaattataca
     1981 gccactatag caactactgc tgaaacatca aaggaaaata gacacagatt agattttaat
     2041 gctttgaaaa aagacgaagc cccttcgaaa gagacgggca aagatagtgc tgtacactta
     2101 gaaactaata gaaagcccca gaatttccct aaggtagcta ccaaatcagt ctccgcagac
     2161 tccaaagttc ataatgacat caagataaca accacagaat ctccaacagc atcgaagaaa
     2221 tcaacttcca caaacgtcac cttacatttt aacgcacaga cagcacagac agcacagccg
     2281 gtgaagaaag aaacggtaaa aaattccgga tactgtgaaa attgtcgtgt aaaatacgaa
     2341 tctttagaac aacacatagt ttctgagaag catttgtctt tcgctgaaaa cgatttaaat
     2401 tttgaggcta ttgactcgtt aattgaaaat ctcagatttc aaatataggg acacgacgta
     2461 aagtgcagta gcttttagtg ataaaatcaa aatagtattg ttccgtttcc attgctgctc
     2521 ggaacaaaaa agctatcaac ggcaatgtta ttgaatcact ttctcattca cccttgttac
     2581 tttcttgcta ttgacttaac tcttatttac tcgtccatat attatcaatt gcatatatat
     2641 acatatacat atatatatta tcatctagat taattaccgt tcatatcata actcattctg
     2701 aacaaataat gatcgagtac ccaccgcggt taatgtccca gttttcattt ttacccgata
//