From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Streptomyces griseus principal sigma factor (hrdB) gene, complete
Date: 31 Dec 92 01:48:27 GMT
Distribution: bionet
Checksum: 20249 5

LOCUS       STMSIGFACT   2127 bp ds-DNA             BCT       18-DEC-1992
DEFINITION  Streptomyces griseus principal sigma factor (hrdB) gene, complete
            cds.
ACCESSION   L08071
KEYWORDS    RNA polymerase sigma-factor; cytoplasmic protein; hrdB gene;
            principal sigma factor.
SOURCE      Streptomyces griseus (sub_species griseus) (library: HUT6037) DNA.
  ORGANISM  Streptomyces griseus
            Prokaryotae; Firmicutes; Streptomycetaceae.
REFERENCE   1  (bases 1 to 2127)
  AUTHORS   Hatada,Y., Shinkawa,H., Okada,M., Kinashi,H. and Nimi,O.
  TITLE     Nucleotide sequence and transcriptional analysis of a principal
            sigma factor gene from Streptomyces griseus
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     mRNA            241..>1907
                     /gene="hrdB"
                     /evidence="EXPERIMENTAL"
     RBS             549..559
                     /gene="hrdB"
     CDS             570..1907
                     /gene="hrdB"
                     /product="principal sigma factor"
                     /codon_start=1
                     /translation="MVSAAESPKRARKSVAAKSPVKRTATKTVAAKTTVTRTVAATAA
                     PAVESADAADDAVAAAPAKKTAAKKATAKKAAAKKTTAKKTAAKKSGKQDDEILDGDE
                     AAEEVKAGKGEEEEGEGENKGFVLSDDDEDDAPAQQVAVAGATADPVKDYLKQIGKVP
                     LLNAEQEVELAKRIEAGLFAEDKLANADKLAPKLKRELEIIAEDGRRAKNHLLEANLR
                     LVVSLAKRYTGRGMLFLDLIQEGNLGLIRAVEKFDYTKGYKFSTYATWWIRQAITRAM
                     ADQARTIRIPVHMVEVINKLARVQRQMLQDLGREPTPEELAKELDMTPEKVIEVQKYG
                     REPISLHTPLGEDGDSEFGDLIEDSEAVVPADAVSFTLLQEQLHSVLDTLSEREAGVV
                     SMRFGLTDGQPKTLDEIGKVYGVTRERIRQIESKTMSKLRHPSRSQVLRDYLD"
BASE COUNT      388 a    738 c    721 g    280 t
ORIGIN      
        1 ggtacccgcg ccgcgcgagc actgaccggg gcgcggccgg gtgcgtgggc cccggtgcgt
       61 gcgtccggcg ccgccgggtc gcacgggtcc ggcggcataa ttgtgggggg atggcacctt
      121 cggcccattt cgtcacgtat gaggtgtgac tcgggccacg cggattggcc gtaacactcc
      181 tcgaagcagc gcgatgactt aagaggtgag cgtcgcggaa ggaatacagc agccctcggg
      241 tgcgctgtcc agttccgagg ccaaacccgc gccgtcggcg acatccccag tcgacggtcg
      301 tcggttccag ccctctccag ggcggggccg gaagccgttt ccatcgttcc gagaggttgt
      361 tcgtgtcggc cagcacatcc cgtacgctcc cgccggagat cgccgagtcc gagtctgtga
      421 tggcgctcat cgagcgggga aaggctgatg ggcagatcgc cggcgatgac gtgcgtcggg
      481 ccttcgaggc tgaccagatt ccgccaaccc agtggaagaa cgttctgcgc agcctcaacc
      541 agatcctcga ggaagagggt gtgacgctga tggtcagtgc cgcggagtcg ccgaagcgcg
      601 cccgcaagag cgtcgcagcg aagagcccgg tcaagcgcac cgccaccaag accgtcgcgg
      661 cgaagacgac cgtgaccagg accgtcgcgg ccaccgccgc cccggcggtc gagagcgcgg
      721 acgcggccga cgacgccgtc gccgccgccc ccgccaagaa gacggcagcc aagaaggcca
      781 cggccaagaa ggcagccgcc aagaagacga cggccaagaa gaccgccgcc aagaagtccg
      841 gcaagcagga cgacgagatc ctcgacggcg acgaggcggc cgaggaagtc aaggccggca
      901 agggtgagga agaggagggc gagggcgaga acaagggctt cgtcctctcc gacgacgacg
      961 aggacgacgc gcccgcgcag caggtcgccg tcgccggtgc caccgccgac ccggtcaagg
     1021 actacctgaa gcagatcggc aaggttcccc tcctcaacgc cgagcaggag gtcgagctcg
     1081 ccaagcgcat cgaggccggt ctcttcgccg aggacaagct ggcgaacgcc gacaagctcg
     1141 cgccgaagct caagcgcgag ctggagatca tcgccgagga cggccgccgg gccaagaacc
     1201 acctcctgga ggccaacctc cgtctggtgg tctccctggc caagcgctac acgggtcgcg
     1261 gcatgctctt cctggacctg atccaggagg gcaacctcgg tctgatccgc gcggtcgaga
     1321 agttcgacta caccaagggc tacaagttct ccacgtacgc cacctggtgg atccgtcagg
     1381 cgatcacccg cgcgatggcc gaccaggccc gcaccatccg tatcccggtg cacatggtcg
     1441 aggtcatcaa caagctcgcg cgcgtgcagc gccagatgct ccaggacctg ggccgcgagc
     1501 ccaccccgga ggagctggcc aaggaactcg acatgacccc cgagaaggtc atcgaggtcc
     1561 agaagtacgg tcgcgagccg atctcgctgc acaccccgct cggtgaggac ggcgacagcg
     1621 agttcggtga cctgatcgag gactccgagg ccgtcgtccc ggcggacgcg gtcagcttca
     1681 cgctcctcca ggagcagctc cactccgtgc tcgacacgct ctccgagcgt gaggcgggcg
     1741 tggtctcgat gcgcttcggt ctcaccgacg gccagccgaa gacgctcgac gagatcggca
     1801 aggtctacgg cgtgacgcgt gagcgcatcc gtcagatcga gtcgaagacg atgtcgaagc
     1861 tccgccaccc gtcccgctcg caggtgctgc gcgactacct cgactagatc gaggagatcc
     1921 accggccgag ggcccggaac cccgagggga tccgggccct ccggcgtgtc ctcgtgggcg
     1981 gacggtgacc ggctggatca cgctgggtgg accgttaccg cctcagcgtc aggagcctcc
     2041 atgccccgtt ccctcgtccg cgccctgacc ggggtgctcg ccctgaccgc cgccgcgacc
     2101 gcgcgcccct cgccgcgccc tccgcgg
//