From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: D.pseudoobscura Adh gene for alcohol dehydrogenase
Date: 28 Dec 92 21:26:58 GMT
Distribution: bionet
Checksum: 28440 8

LOCUS       DROADHGN31   3475 bp ds-DNA             INV       01-DEC-1992
DEFINITION  D.pseudoobscura Adh gene for alcohol dehydrogenase
ACCESSION   X62211
KEYWORDS    ADH gene; alcohol dehydrogenase.
SOURCE      Drosophila pseudoobscura DNA.
  ORGANISM  Drosophila pseudoobscura
            Eukaryota; Animalia; Metazoa; Arthropoda; Uniramia; Insecta;
            Pterygota; Neoptera; Holometabola; Diptera; Brachycera;
            Cyclorrhapha; Schizophora; Drosophiloidea; Drosophilidae.
REFERENCE   1  (bases 1 to 3475)
  AUTHORS   Schaeffer,S.W. and Miller,E.L.
  TITLE     Estimates of gene flow in Drosophila pseudoobscura determined from
            nucleotide sequence analysis of the alcohol dehydrogenase region
  JOURNAL   Genetics 132, 471-480 (1992)
  STANDARD  full automatic
COMMENT     *source: strain=GB69; *source: isolate=Gundlach Bundschu Winery,
            Sonoma Valley, *source: California; *source: chromosome=IV section
            88;
               From EMBL    entry DPADHGN31;  dated 16-OCT-1992.
FEATURES             Location/Qualifiers
     mRNA            join(40..119,916..1021,1085..1489,1550..1965)
                     /note="adult mRNA (Adh)"
     mRNA            join(883..1021,1085..1489,1550..1965)
                     /note="larval mRNA (Adh)"
     promoter        8..14
                     /note="adult promoter (Adh)"
     prim_transcript 40..1965
                     /note="adult primary transcript (Adh)"
     exon            40..119
                     /note="adult exon 1a"
     intron          120..915
                     /note="adult intron"
     promoter        847..854
                     /note="larval promoter (Adh)"
     prim_transcript 883..1965
                     /note="larval primary transcript (Adh)"
     exon            883..1021
                     /note="larval exon 1"
     exon            916..1021
                     /note="adult exon 1b"
     intron          1022..1084
                     /number=1
     exon            1085..1489
                     /number=2
     intron          1490..1549
                     /number=2
     exon            1550..1965
                     /number=3
     polyA_signal    1942..1947
     polyA_site      1965
     CDS             join(929..1021,1085..1489,1550..1816)
                     /EC_number="1.1.1.1"
                     /gene="Adh"
                     /product="alcoholdehydrogenase"
                     /codon_start=1
                     /translation="MSLTNKNVVFVAGLGGIGLDTSRELVKRNLKNLVILDRIDNPAA
                     IAELKAINPKVTITFYPYDVTVPVAETTKLLKTIFAQVKTIDVLINGAGILDDHQIER
                     TIAVNYTGLVNTTTAILDFWDKRKGGPGGIICNIGSVTGFNAIYQVPVYSGSKAAVVN
                     FTSSLAKLAPITGVTAYTVNPGITKTTLVHKFNSWLDVEPRVAEKLLEHPTQTSQQCA
                     ENFVKAIELNKNGAIWKLDLGTLEPITWTQHWDSGI"
BASE COUNT      976 a    788 c    795 g    916 t
ORIGIN      
        1 cgtagtgtat ttaaggcact gcacatgtcg aggaccacga ttattgtcta cagtgcagtc
       61 agtagttgtc aggtgcagca ggcggaccag catttcttta aatctactca atcgatcaag
      121 taagtaactg taatccaagt acagtcggcc catcccactc ggttctgccg taactcaaga
      181 aatttcttga tagcgcagtt ccagttgtaa aacgtcgttt aaaaaaacat ctcgttagag
      241 cttgggttga aaaacgttcg agttgggcgt agaaaagttc aaatataaaa tatcaaaaag
      301 agaaaatatc ttcccaaagc agctgtccat tttttgttaa agtgatagat caaaaattcg
      361 tatgacgtat ttgccaagtg ctctttatgc aaataatttc ctcaattcta attctacaag
      421 aagcactttt ggctcttgtc cagaaaaaac aatttgttta cacttttcgg ttgataacag
      481 aggcggaccg acggctaagt aacataacag tcgcctgtta agtaatgccc tcactctctg
      541 ttagatggcc gcgcgtgtcg actccgatag accagcagac acgcagtagt tttcctcaag
      601 aacctgaact ctaaacatag acataattta cgataccttg agactcaaat aaaaatacaa
      661 gtatgagtgg tattctattc ctgattagac tcgcacacac acacacgtac gagtattaac
      721 ataaatttgc cggcaaataa ccaaaacaaa acgaacgcaa acgatccgaa cacgtaatgc
      781 gagagataag aaacagaaaa gctcgacgtg aaagaagctt ctggcggaag ctcttgccga
      841 actccctata aatacgagac agaaaaccag aataaatata ataacaaact gttgaattgt
      901 cccatcgatt cccagatcaa aagacagaat gtctctcacc aacaagaacg tcgttttcgt
      961 ggccggtctg ggcggcattg gcctggacac cagccgggag ttggtcaagc gtaatctgaa
     1021 ggtaagagag aacgaattcc atggaatcta tggaatccta aatttaaaaa ttcatttatt
     1081 ttagaacctg gtcatcctgg atcgcattga caatccggct gccattgccg aactgaaggc
     1141 aatcaatccc aaggtgacca tcaccttcta tccctacgat gtgactgtgc ccgtcgctga
     1201 gaccaccaag ctcctgaaga ccatctttgc ccaggtgaag acaatcgatg tcctgatcaa
     1261 cggtgctggc atcctggacg atcatcagat cgagcgcacc attgccgtta actacacggg
     1321 cctggttaac accaccacag ccattctgga cttctgggac aagcgcaagg gcggcccagg
     1381 cggcatcatt tgcaacattg gctccgtcac cggtttcaat gccatctacc aggtgcccgt
     1441 ttactccggc tccaaggcgg cggtggttaa ctttacctcc tccctggcgg taagtaatcc
     1501 aacgtttttc ctactcccca aaactaaata tttctttgaa attccataga aactggcccc
     1561 cattactggt gtcactgctt acactgtcaa tcctggcatc actaagacca ctctggtcca
     1621 caaattcaac tcgtggctgg atgtggagcc ccgtgtggcg gagaagctgc tcgagcatcc
     1681 cacccagacc tctcagcagt gcgccgagaa ctttgtgaag gccattgagc tgaacaagaa
     1741 cggtgccatc tggaagttgg atttgggcac cttggagccc atcacatgga cccagcactg
     1801 ggactcgggc atctaaacca tcccagagac tctatgggac atggcgtttt agcttttagt
     1861 ttcttttttt ccactcaatt gttacgtata tacatacaca tatggcaata aggctgattt
     1921 gaaccccgtt ttgaatatga taatacaaaa ttatatttga gaaatttcaa caaaatcgat
     1981 aagagctata gctctctttt aattaaaaaa aactagataa aaagagcaat ggtcgtcagt
     2041 ggtggtcagt ggtcgttgtg gcctctatcg atttccacac aaaaacttta ctgtttagta
     2101 atagaaaaga acttgagaaa ggcagccaaa agaatgtacg atctgacggg taagcatgtc
     2161 tgctatgtgg ctgactgcgg tggcattgca ctggagacca gcaaggttct catgaccaag
     2221 aatatagcgg tgagtggtag agtggtgtag agagtggaga gtggttctgg ttcttaaaca
     2281 agtctctagt ctctagtctc tagtctcttg gctgctgctg gtagctgtcg aaactaatta
     2341 agttaatgac tttttcatgt tttgttattg tccgctgact tgctgttcaa agctacaatt
     2401 aagtttatgt tttgagtttg atctactttt gcttaaggga agtactttcg attacggatt
     2461 gctggcatat aaaaatagat accaaggaat gtgcatcata tcatattgat tctatcttga
     2521 ttctttgccc agaaactggc catccttcag agcgtggaaa accccccggc aatcgctcag
     2581 ttacaatcga ttaagcacag cacacagatc ttcttctgga ccttcgatgt gaccatggcc
     2641 cgcgaggaga tgaagaagta ctttgatgag gtcatggtcc agatggacta catcgatgta
     2701 ctgatcaatg gggccaccct gtgcgatgag cggaacattg atgccaccat caatacgaat
     2761 ctgaccggca tgatgaacac cgtggccact gtgctgccct atatggaccg taagatgggc
     2821 ggttcgggtg gactgattgt gaatgtcacc tccgtcatag gactggatcc atcgccagtc
     2881 ttttgtgcgt acagtgcctc aaagtttggt gtgattgggt tcaccagaag tctagcggtg
     2941 agtgggcgag atcctaactt gaaaatgctc tctaatgtct tccattcatt cgcaggatcc
     3001 cctgtattac acccaaaatg gtgtggctgt catggccgtc tgttgtggtc ccaccaaagt
     3061 gtttgtcgat cgggaactga ctgccttcct ggaatacggc caaacctttg ccgatcgcct
     3121 gcgtcgtgcc ccctgccaat cgacggccgt ctgcggccag aatatagtaa atgccatcga
     3181 gagatcggag aacggacaga tctggattgc cgacaagggc ggcctggagt cggtggccct
     3241 ccactggtat tggcatatgg ccgatcagtt tgtgaactac atgcagagca ctgacgatga
     3301 ggatcaggag ttttttttgg ggcagcgatg aggcatgcct tgatccacgg aataaaacat
     3361 caaatgtgtc acaaaaagta ggcaacttgt ttgtgggttt ggaaatagat ttgaattatt
     3421 ttgaggtcat atgaataatg acataaatga gggtgcctga aagtatgcca agttt
//