From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Pisum sativum (clone pCLp) nuclear encoded precursor to chloroplast
Date: 28 Jan 93 05:48:04 GMT
Distribution: bionet
Checksum: 56347 7

LOCUS       PEAPCLP      3239 bp ss-mRNA            PLN       27-JAN-1993
DEFINITION  Pisum sativum (clone pCLp) nuclear encoded precursor to chloroplast
            protein mRNA, complete cds.
ACCESSION   L09547
KEYWORDS    ATP-dependant protease; nuclear-encoded precurso protein;
            regulatory subunit.
SOURCE      Pisum sativum (library: lamda-gt11 of Steven Gantt) seedling cDNA
            to mRNA.
  ORGANISM  Pisum sativum
            Eukaryota; Plantae; Embryobionta; Magnoliophyta; Magnoliopsida;
            Rosidae; Fabales; Fabaceae.
REFERENCE   1  (bases 1 to 3239)
  AUTHORS   Moore,T. and Keegstra,K.
  TITLE     Characterization of a cDNA clone encoding a chloroplast-targeted
            Clp homologue
  JOURNAL   Plant Mol. Biol. (1993) In press
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     CDS             52..2820
                     /function="regulatory subunit of an atp-dependent
                     protease"
                     /citation=[1]
                     /note="hypothetical protein function based on homology to
                     known sequences; putative"
                     /product="nuclear encoded precursor to chloroplast
                     protein"
                     /codon_start=1
                     /translation="MARVLAQSLSVPGLVAGHKDSQHKGSGKSKRSVKTMCALRTSGL
                     RMSGFSGLRTFNHLNTMMRPGLDFHSKVSKAVSSRRARAKRFIPRAMFERFTEKAIKV
                     IMLAQEEARRLGHNFVGTEQILLGLIGEGTGIAAKVLKSMGINLKDARVEVEKIIGRG
                     SGFVAVEIPFTPRAKRVLELSQEEARQLGHNYIGSEHLLLGLLREGEGVAARVLENLG
                     ADPTNIRTQVIRMVGESADSVTATVGSGSSNNKTPTLEEYGTNLTKLAEEGKLDPVVG
                     RQPQIERVTQILGRRTKNNPCLIGEPGVGKTAIAEGLAQRIANGDVPETIEGKKVITL
                     DMGLLVAGTKYRGEFEERLKKLMEEIKQSDDIILFIDEVHTLIGAGAAEGAIDAANIL
                     KPALARGELQCIGATTLDEYRKHIEKDPDLERRFQPVKVPEPTVDETIQILKGLRERY
                     EIHHKLRYTDEALIAAAQLSYQYISDRFLPDKAIDLVDEAGSRVRLQHAQLPEEAKEL
                     DKEVRKIVKEKEEYVRNQDFEKAGELRDKEMDLKAQISALIEKGKEMSKAETETADEG
                     PIVTEVDIQHIVSSWTGIPVDKVSADESDRLLKMEDTLHKRIIGQDEAVQAISRAIRR
                     ARVGLKNPNRPIASFIFSGPTGVGKSELAKALAAYYFGSEEAMIRLDMSEFMERHTVS
                     KLIGSPPGYVGYTEGGQLTEAVRRRPYTVVLFDEIEKAHPDVFNMMLQILEDGRLTDS
                     KGRTVDFKNTLLIMTSNVGSSVIEKGGRRIGFDLDYDEKDSSYNRIKSLVTEELKQYF
                     RPEFLNRLDEMIVFRQLTKLEVKEIADIMLKEVFQRLKTKEIELQVTERFRDRVVDEG
                     YNPSYGARPLRRAIMRLLEDSMAEKMLAREIKEGDSVIVDVDSDGKVIVLNGSSGTPE
                     SLPEALSI"
BASE COUNT      965 a    577 c    822 g    875 t
ORIGIN      
        1 ttttattttt cttgcttgat tcgttttatc tggggaactg cttccggaat catggctaga
       61 gttttggctc agtcacttag tgttcctggc ctagtggctg ggcacaagga cagtcaacat
      121 aagggatcgg gaaaatctaa gagatcagtc aagacgatgt gtgctttgcg aacaagcgga
      181 ttaagaatgt caggtttctc aggacttcgt actttcaatc atttgaatac tatgatgaga
      241 cctggactgg attttcactc aaaggtatca aaggcagttt cttcacggcg ggcaagggct
      301 aagagattca ttcctagagc catgtttgag cgtttcaccg agaaagcaat caaagtgata
      361 atgctagctc aggaggaagc aagacgtctt ggtcacaatt ttgtcggaac tgagcagatt
      421 cttttgggtc ttattggtga aggcactggt atcgccgcca aggttttaaa gtctatggga
      481 atcaacctta aagatgcacg tgttgaagtg gagaagataa ttggaagggg tagtggattt
      541 gttgctgtcg agattccatt tactccccgt gcaaagcgtg tattggaact ttcacaggag
      601 gaagctcgcc aacttggtca caattatatt ggatcagagc acttgcttct aggtcttctt
      661 cgagagggtg agggtgtagc agcacgtgtt cttgaaaact tgggcgctga tcctactaat
      721 attcgcacac aggttattcg catggtgggt gagagtgctg acagtgttac cgctactgtt
      781 ggctcaggaa gtagtaacaa caaaacgcca actttggagg agtatggaac caatttgacc
      841 aagctagcag aagagggaaa gttggatcct gttgtgggaa ggcagccaca gattgagcgt
      901 gtcacccaaa ttttaggccg ccgcaccaaa aataatcctt gtcttattgg agagcctggt
      961 gttgggaaga cagcaattgc tgaaggtctt gctcagcgga ttgctaatgg tgatgtacct
     1021 gaaacaatcg aaggaaaaaa ggttataacc ctcgatatgg gtctgcttgt agctggaacc
     1081 aaataccgtg gtgagtttga ggagaggttg aagaaactga tggaagaaat caaacaaagt
     1141 gatgatatta tactttttat cgatgaagtg cacactctaa ttggagcagg agcagctgaa
     1201 ggtgctattg atgctgctaa catactaaaa ccagctcttg caagaggtga actacagtgt
     1261 attggggcca caacattgga cgaatacagg aagcatattg aaaaagaccc agatttggag
     1321 agacgattcc agccagttaa agtaccagaa ccaactgtag atgaaaccat acaaatactg
     1381 aaaggactta gagaacgtta tgagattcac cacaagctcc gctatactga tgaggctctt
     1441 atagctgctg cgcaactttc gtaccagtat atcagtgatc gatttttgcc tgacaaagct
     1501 atagatttgg ttgatgaagc tggttcacga gtccgtcttc aacatgcaca gttgccagaa
     1561 gaggcaaaag aacttgacaa ggaggtcagg aagattgtta aggagaaaga ggaatatgtt
     1621 cgcaaccaag actttgaaaa ggctggagaa ctgcgtgata aagaaatgga tctcaaggca
     1681 cagatctcag cacttataga gaaaggcaag gagatgagca aagcagagac tgagacagca
     1741 gatgaaggtc ccattgtgac tgaagttgac attcaacata ttgtgtcctc ctggacaggc
     1801 attcctgttg ataaagtctc agcagatgaa tctgatcgtc tcctcaagat ggaagatact
     1861 ttacacaagc gaatcattgg tcaggatgaa gcagtgcaag ccattagccg tgctatccgt
     1921 cgagcccgag ttggattgaa gaaccctaat cgtccaattg ccagcttcat cttttctggt
     1981 ccaactggtg tggggaagtc tgaattggcc aaagcattgg ctgcatacta ctttggctct
     2041 gaagaagcca tgattcgtct tgacatgagt gaatttatgg aaaggcacac agtctccaaa
     2101 cttattggtt cacctccagg atatgttggt tacaccgaag gcggtcaatt gactgaagca
     2161 gttcgacgtc gtccttacac tgttgtactc tttgatgaga ttgagaaagc ccatcctgat
     2221 gtattcaaca tgatgcttca aatccttgaa gatggaagac taacagacag caagggtaga
     2281 actgtggatt tcaagaacac acttcttata atgacatcaa atgttggaag cagtgtgatt
     2341 gagaaaggag gccgccgaat tggatttgat ttggattacg atgagaaaga tagcagttat
     2401 aatagaatca agagcttggt gactgaggag ctaaaacaat acttcaggcc agagtttttg
     2461 aataggttgg atgaaatgat tgttttcaga caactcacaa aattggaagt gaaggagata
     2521 gcagacataa tgctcaagga ggtgtttcaa agacttaaga ccaaagaaat tgaacttcaa
     2581 gtcacagaaa gatttaggga cagggtggtt gatgaaggtt ataatcctag ttacggagct
     2641 aggcctctaa gaagagccat aatgcgactt ttggaggata gcatggctga gaagatgctc
     2701 gccagagaga tcaaagaggg tgattctgtc atagtggatg ttgattctga tggtaaagtg
     2761 attgttctca acggtagcag cggcactcca gagtcgttac cagaggctct ttctatataa
     2821 tgtggccgag ttacgatttc ccttagcagc cctagcaaat caagttataa aaggggttta
     2881 catcacagga acaggtggga taagggaaaa ccaaaaaggg tgtgtgaatg atgaaaaaat
     2941 attctgtgta tggcatgccc cggacatgtt catcttcatc catggctatt tcgtcggaag
     3001 ctggagtatt tttatgtttt ctctgataga ctataacatc ccagctttgt tgtatagtat
     3061 tgctatggcg tatttattga tcgacatgta atgacaaata cttgtatgac aacaacaaat
     3121 tcaaggtaat tagatctgtt ggaaatatgt gacctagtat acagtgggtt gcatgttttt
     3181 gaggttcatg tcataggttg tattcttggt tttatgagtc cgactttgtt ccatttatc
//