From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: T.cruzi gene encoding trypanothione reductase.
Date: 28 Jul 92 10:08:06 GMT
Distribution: bionet
Checksum: 03147     5

LOCUS       TRBTRYRED    1479 bp ds-DNA             INV       28-JUL-1992
DEFINITION  T.cruzi gene encoding trypanothione reductase.
ACCESSION   Z13958
KEYWORDS    trypanothione reductase.
SOURCE      Trypanosoma cruzi DNA.
  ORGANISM  Trypanosoma cruzi
            Eukaryota; Animalia; Protozoa; Sarcomastigophora; Mastigophora;
            Kinetoplastida; Trypanosomatina; Trypanosomatidae.
REFERENCE   1  (bases 1 to 1479)
  AUTHORS   Borges,A. and Fairlamb,A.H.
  TITLE     Mutagenesis of the redox-active cysteines in the trypanothione
            reductase from Trypanosoma cruzi. Implications for the mechanism of
            catalysis.
  JOURNAL   Unpublished (1992)
  STANDARD  full staff_review
REFERENCE   2  (bases 1 to 1479)
  AUTHORS   Fairlamb,A.H.
  JOURNAL   Unpublished (1992)
  STANDARD  full staff_review
COMMENT     *source: strain=Silvio; *source: clone=pBTR; *source: isolate=
            X10/1;
            From EMBL    entry TCTRYRED;  dated 29-JUN-1992.
FEATURES             Location/Qualifiers
     CDS             1..1479
                     /function="reduction of trypanothione disulphide"
                     /EC_number="1.6.4.8"
                     /note="pBTRwas constructed by ligation of a PCR
                     fragmentamplified from genomic DNA into the
                     expressionvector pBSTNAV."
                     /product="trypanothionereductase"
                     /evidence=EXPERIMENTAL
                     /codon_start=1
                     /translation="MMSKIFDLVVIGAGSGGLEAAWNAATLYKKRVAVIDVQMVHGPP
                     FFSALGGTCVNVGCVPKKLMVTGAQYMEHLRESAGFGWEFDRTTLRAEWKNLIAVKDE
                     AVLNINKSYEEMFRDTEGLEFFLGWGSLESKNVVNVRASADPASAVKERLETENILLA
                     SGSWPHMPNIPGIEHCISSNEAFYLPEPPRRVLTVGGGFISVEFAGIFNAYKPKDGQV
                     TLCYRGEMILRGFDHTLREELTKQLTANGIQILTKENPAKVELNADGSKSVTFESGKK
                     MDFDLVMMAIGRSPRTKDLQLQNAGVMIKNGGVQVDEYSRTNVSNIYAIGDVTNRVML
                     TPVAINEAAALVDTVFGTTPRKTDHTRVASAVFSIPPIGTCGLIEEVASKRYEVVAVY
                     LSSFTPLMHKVSGSKYKTFVAKIITNHSDGTVLGVHLLGDNAPEIIQGIGICLKLNAK
                     ISDFYNTIGVHPTSAEELCSMRTPSYYYVKGEKMEKPSEASL"
                     /citation=[1]
                     /citation=[2]
BASE COUNT      349 a    359 c    411 g    360 t
ORIGIN
        1 atgatgtcaa aaatttttga tttggttgtc attggcgccg gctcgggcgg actggaggct
       61 gcttggaacg cggcgacact ctacaagaag cgggttgcgg tgattgatgt tcagatggtt
      121 cacgggcccc cgtttttttc tgctctaggc ggtacgtgtg tcaatgttgg ctgcgttccg
      181 aagaaattga tggttacagg ggcccaatac atggagcacc tgcgcgagtc tgctgggttc
      241 gggtgggagt ttgatcgcac cactctcagg gcggaatgga agaaccttat tgctgtcaag
      301 gacgaggcgg tgctgaatat caacaagagt tatgaggaga tgtttcggga cacggagggt
      361 ctggagtttt tcctgggctg gggatcactg gagtcaaaga atgtcgtcaa tgttcgcgcg
      421 agtgccgacc cggccagcgc agtgaaggag cgcctggaga cggagaacat tctacttgcc
      481 agtggttcgt ggccgcacat gccaaacatc cctggtattg agcattgcat cagcagcaat
      541 gaggcattct acctgccgga gccaccgcgt cgtgtcctca ctgtcggcgg agggttcatt
      601 tccgtggagt ttgccggcat ttttaacgcc tacaagccga aggacggaca agtgacgttg
      661 tgctaccgcg gtgaaatgat ccttcgtggc tttgaccaca ctctccgtga ggaactcaca
      721 aagcagctca ccgccaacgg cattcaaatc cttacgaagg aaaatccggc caaggtggag
      781 ttgaacgcgg atggcagcaa aagtgttact ttcgagagcg gcaaaaagat ggactttgat
      841 cttgtcatga tggcgattgg ccgttctccc cgaacaaagg atttacagct gcaaaacgcc
      901 ggcgtcatga tcaagaacgg tggtgtgcag gtggacgagt actcgcgcac gaatgtttcc
      961 aacatttacg ccatcggtga cgtcacaaat cgtgtcatgt tgacacccgt tgccataaat
     1021 gaagccgctg cccttgtgga tacagtcttt ggtaccactc cgcgaaagac ggaccacacc
     1081 cgtgtggcga gtgccgtctt ctctattcct ccaattggta cctgcggtct cattgaagag
     1141 gttgcatcca agcgctacga ggtggtggcg gtataccttt ccagctttac cccgctcatg
     1201 cacaaagtca gcggatcaaa gtataagact tttgttgcaa agataattac caaccactcc
     1261 gatggcactg ttcttggtgt acatcttctt ggggacaatg ccccagaaat catccaaggt
     1321 attggtatct gtctcaagtt aaacgccaaa atatccgact tctacaacac tattggtgtg
     1381 catcccacaa gtgcggagga gctgtgctcc atgcgcactc cttcttacta ctatgttaaa
     1441 ggtgagaaga tggaaaagcc ttcagaggca tctctgtaa
//