From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Mouse myelin proteolipid protein mRNA, complete cds.
Date: 6 Nov 92 18:32:29 GMT
Distribution: bionet
Checksum: 47034 8

LOCUS       MUSPLPM      2892 bp ss-mRNA            ROD       02-NOV-1992
DEFINITION  Mouse myelin proteolipid protein mRNA, complete cds.
ACCESSION   M15442 M25442
KEYWORDS    alternative splicing; myelin proteolipid protein.
SOURCE      Mouse brain, cDNA to mRNA, clones lambda-PLP[1,2].
  ORGANISM  Mus musculus
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Rodentia; Myomorpha; Muridae; Murinae.
REFERENCE   1  (bases 1 to 2892)
  AUTHORS   Hudson,L.D.
  JOURNAL   Unpublished (1987) Bldg 36, Rm 4A01, NIH, Bethesda Md 20892
  STANDARD  full automatic
REFERENCE   2  (bases 10 to 2892)
  AUTHORS   Hudson,L.D., Berndt,J.A., Puckett,C., Kozak,C.A. and Lazzarini,R.A.
  TITLE     Aberrant splicing of proteolipid protein mRNA in the dysmyelinating
            jimpy mutant mouse
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 84, 1454-1458 (1987)
  STANDARD  full automatic
COMMENT     Draft entry and computer-readable copy of sequence for [2],[1]
            kindly provided by L.Hudson, 27-APR-1987.
               The jimpy mutation is caused by a deletion of exon 5, which is
            exactly 74 base pairs.  A DM-20 protein is formed by alternative
            splicing of the PLP gene.
               An alternative minor polyadenylation signal is located at
            positions 2297-2302.
FEATURES             Location/Qualifiers
     exon            <148..495
                     /number=1
                     /note="DM-20 protein"
     exon            <148..769
                     /number=1
                     /note="jimpy mutant proteolipid protein"
     mRNA            <1..>2892
                     /note="PLP mRNA (major alt.)"
     mRNA            <1..2297
                     /note="PLP mRNA (minor alt.)"
     exon            601..>981
                     /note="DM-20 protein"
     mutation        769..844
                     /note="c-70bp-t in wt; ct in jimpy mutant"
     exon            844..>953
                     /number=2
                     /note="jimpy mutant proteolipid protein"
     repeat_region   2535..2698
                     /note="purine repeat region"
     CDS             join(148..495,601..980)
                     /partial
                     /note="DM-20 protein"
                     /codon_start=1
                     /translation="MGLLECCARCLVGAPFASLVATGLCFFGVALFCGCGHEALTGTE
                     KLIETYFSKNYQDYEYLINVIHAFQYVIYGTASFFFLYGALLLAEGFYTTGAVRQIFG
                     DYKTTICGKGLSATFVGITYALTVVWLLVFACSAVPVYIYFNTWTTCQSIAFPSKTSA
                     SIGSLCADARMYGVLPWNAFPGKVCGSNLLSICKTAEFQMTFHLFIAAFVGAAATLVS
                     LLTFMIAATYNFAVLKLMGRGTKF"
     CDS             join(148..769,844..952)
                     /partial
                     /note="jimpy mutant proteolipid protein"
                     /codon_start=1
                     /translation="MGLLECCARCLVGAPFASLVATGLCFFGVALFCGCGHEALTGTE
                     KLIETYFSKNYQDYEYLINVIHAFQYVIYGTASFFFLYGALLLAEGFYTTGAVRQIFG
                     DYKTTICGKGLSATVTGGQKGRGSRGQHQAHSLERVCHCLGKWLGHPDKFVGITYALT
                     VVWLLVFACSAVPVYIYFNTWTTCQSIAFPSKTSASIGSLCADARMYVPNDLPPVYCC
                     VCGCCGHTSFPAHLHDCCHLQLRRP"
     CDS             148..981
                     /product="myelin proteolipid protein"
                     /codon_start=1
                     /translation="MGLLECCARCLVGAPFASLVATGLCFFGVALFCGCGHEALTGTE
                     KLIETYFSKNYQDYEYLINVIHAFQYVIYGTASFFFLYGALLLAEGFYTTGAVRQIFG
                     DYKTTICGKGLSATVTGGQKGRGSRGQHQAHSLERVCHCLGKWLGHPDKFVGITYALT
                     VVWLLVFACSAVPVYIYFNTWTTCQSIAFPSKTSASIGSLCADARMYGVLPWNAFPGK
                     VCGSNLLSICKTAEFQMTFHLFIAAFVGAAATLVSLLTFMIAATYNFAVLKLMGRGTK
                     F"
BASE COUNT      835 a    607 c    677 g    772 t      1 others
ORIGIN      3 bp upstream of EcoRI site; chromosome XF1.
        1 gggaattccg aggacaaaga tactcagaga gaaaaagtaa aggacagaag aaggagactg
       61 gagagaccag gatccttcca gctgagcaaa gtcagccgca aaacagacta gccaacaggc
      121 tacaattgga gtcagagtgc caaagacatg ggcttgttag agtgttgtgc tagatgtctg
      181 gtaggggccc cctttgcttc cctggtggcc actggattgt gtttctttgg agtggcactg
      241 ttctgtggat gtggacatga agctctcact ggtacagaaa agctaattga gacctatttc
      301 tccaaaaact accaggacta tgagtatctc attaatgtga ttcatgcttt ccagtatgtc
      361 atctatggaa ctgcctcttt cttcttcctt tatggggccc tcctgctggc tgagggcttc
      421 tacaccaccg gcgctgtcag gcagatcttt ggcgactaca agaccaccat ctgcggcaag
      481 ggcctgagcg caacggtaac agggggccag aaggggaggg gttccagagg ccaacatcaa
      541 gctcattctt tggagcgggt gtgtcattgt ttgggaaaat ggctaggaca tcccgacaag
      601 tttgtgggca tcacctatgc cctgactgtt gtatggctcc tggtgtttgc ctgctcggct
      661 gtacctgtgt acatttactt caatacctgg accacctgtc agtctattgc cttccctagc
      721 aagacctctg ccagtatagg cagtctctgc gctgatgcca gaatgtatgg tgttctccca
      781 tggaatgctt tccctggcaa ggtttgtggc tccaaccttc tgtccatctg caaaacagct
      841 gagttccaaa tgaccttcca cctgtttatt gctgcgtttg tgggtgctgc ggccacacta
      901 gtttccctgc tcaccttcat gattgctgcc acttacaact tcgccgtcct taaactcatg
      961 ggccgaggca ccaagttctg agctcccata gaaactcccc tttgtctaat agcaaggctc
     1021 taaccacaca gcctacagtg ttgtgtttta actctgcctt tgccactgat tggccctctt
     1081 cttacttgat gagtataaca agaaaggaga gtcttgcagt gattaatctc tctctgtgga
     1141 ctctccctct tagtacctct tttagtcatt ttgctccaca gcaggctcct gctagaaatg
     1201 ggggatgcct gagaaggtga ctccccagct gcaagtcgca gaggagtgaa agctctaatt
     1261 gattttgcaa gcatctcctg aagaccagga tgtgcttcct tctcaaaggg cacttccaac
     1321 tgaggagagc agaacggaaa ggttctcagg tagagagcag aaatgtccct ggtcttcttg
     1381 ccatcagtag gagtcaaata cattctcttt gatgcacaaa accaagaact cactcttacc
     1441 ttcctgtttc cactgaagac agaagaaaat aaaaagaatg ctagcagagc aatatagcat
     1501 ttgcccaaat ctgcctcctg cagctgggag aagggtgtca aagcaaggat ctttcgccct
     1561 tagaaagaga gctctgacgc cagtggcaat ggactattta agccctaact cagccaacct
     1621 tcttacggca attagggagc acagtgcctg tatagacaaa gcggggcgga gggggggggc
     1681 atcatctgtc cttatagctc attaggaaga gaaacagtgt tgtcaggatc atctcactcc
     1741 cttctccttg ataacagcta ccatgacaac cttgtggttt ccaaggagct gagaatagaa
     1801 aggaactagc ttatttgaaa taagactgtg acctaaggag catcagttgg tggatgctaa
     1861 aggtgtaatt tgaaatggcc ttcgggtaaa tgcaagatac ttaactcttt ggatagcatg
     1921 tgttcttccc ccacccctat ccgctagttc tggcccctgg cctctggcat aatatcttca
     1981 caatggtgct ttttttcctg gggttttatc cattcactca tagcaggtga ttagacgatc
     2041 ttgattagtt tcatatttcc caattgttta tctcttgttt ggagttgtat cagaaagacc
     2101 tggaggatga ttctttgagc atagttcttt ttgaaaacaa gaaagagaaa ctgggcagaa
     2161 agcatcacaa aaatatttga aattgtacgg tcccatgaaa ttattgggaa ttcccccaag
     2221 tagtctacca tttgtagaac taggcttgat aaatttgaac ctcaatttga ataattggtc
     2281 tggtattttc ttttctaata aatgacagat gattttactt gctaatatta tctcagcatt
     2341 ttgataattt aggcttacca tagaagttac tgtctcttgg tatatatagg tcacataata
     2401 gattctgcca gctgttagct gttcagttca taagcttctc atagagctct ggagccgcag
     2461 agaggacngg cagaatttga aacctaaaga actcccagat tttcaggctt atcctgtatt
     2521 tgttaacaaa gggtgaaaga aagaaagaaa gaaagaaaga aagaaagaaa gaaagaaaga
     2581 aagaaagaaa gaaagaaaga aagaaaaaga aaaggaagga aggaaggaag gaagaaagaa
     2641 agaaagaaag aaagaaagaa agaaagaaag aaagaaagaa agaaagagaa aaaaaaagcc
     2701 cctgatcgaa tttcctgagg aaaagttatt gtagctgttt cattgtagat ttgtgctgtc
     2761 attccccaaa gtgctttctg ctgtgttgaa agagatataa gaatttacaa gaagacactt
     2821 gagacttgtt cttgggccaa tatataaggt aaacaagcag gatgcacaag agtgaggaga
     2881 gctaaggaat tc
//