From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: P22 bacteriophage (from Salmonella typhimurium) injection protein
Date: 29 Dec 92 01:28:47 GMT
Distribution: bionet
Checksum: 24050 6

LOCUS       P22DNAIP     1454 bp ds-DNA             PHG       08-DEC-1992
DEFINITION  P22 bacteriophage (from Salmonella typhimurium) injection protein
            (20) gene, complete cds, and gene 7, 3' end.
ACCESSION   L07556
KEYWORDS    DNA injection protein.
SOURCE      Bacteriophage P22 (strain c1-7, 13-amber H101) DNA.
  ORGANISM  Bacteriophage P22
            Viridae; ds-DNA nonenveloped viruses; Podoviridae.
REFERENCE   1  (bases 1 to 1454)
  AUTHORS   Conlin,C.A., Vimr,E.R. and Miller,C.G.
  TITLE     Oligopeptidase A is required for normal phage p22 development
  JOURNAL   J. Bacteriol. 174, 5869-5880 (1992)
  STANDARD  full automatic
REFERENCE   2  (bases 1 to 1454)
  AUTHORS   Umlauf,B. and Dreiseikelmann,B.
  TITLE     Cloning, sequencing, and overexpression of gene 16 of Salmonella
            bacteriophage P22
  JOURNAL   Virology 188, 495-501 (1992)
  STANDARD  full automatic
REFERENCE   3  (bases 1 to 1454)
  AUTHORS   Adhikari,P. and Berget,P.B.
  TITLE     Sequence of a DNA Injection Gene from Salmonella typhimurium Phage
            P22
  JOURNAL   Unpublished (1993)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     old_sequence    1..306
                     /citation=[1]
                     /note="This span first reported in citation; '' in
                     old_sequence"
     RBS             19..24
                     /gene="20"
                     /note="putative ribosome binding site for gene 20"
     mutation        replace(973,"")
                     /phenotype="amber mutation"
                     /label=amH1025
                     /note="'T' in mutation"
     old_sequence    1067..1454
                     /citation=[2]
                     /note="This span first reported in citation; '' in
                     old_sequence"
     conflict        replace(1199..1200,"")
                     /citation=[2]
                     /note="Two nucleotides reversed at this position in cited
                     sequence; 'cg' in conflict"
     mutation        replace(1249,"")
                     /phenotype="amber mutation"
                     /label=amH1032
                     /note="'T' in mutation"
     conflict        replace(1272,"")
                     /citation=[2]
                     /note="G missing in cited sequence at this position; '' in
                     conflict"
     conflict        replace(1402,"")
                     /citation=[2]
                     /note="G missing in cited sequence at this position; '' in
                     conflict"
     RBS             1437..1441
                     /gene="16"
                     /note="putative"
     CDS             <1..20
                     /gene="7"
                     /codon_start=3
                     /translation="LGSLF"
     CDS             31..1446
                     /gene="20"
                     /product="DNA injection protein"
                     /codon_start=1
                     /translation="MATWQQGINSGGFLAGIGAQNENAPKARDINATLGLIRENNDLA
                     RSGANNVALTGLRGLAGVADIYNQEQQQKALNAFNQVHANAWATGDPSGLFKFAQENP
                     AFVAQAQQAFSGLNEQQRNDMGDLAMKANVALSQGPEAYSKFITDNKDRLNRVGANPD
                     WMIQTGVQNPEQLSHMLTTMSLGALGPEKAFAVQDKMVGREIDRGRLAETIRSNKAGE
                     GLQARGQNITMRGQDMSAATARRGQDLATQRANARTISGSEGNRVVQLADGRTVSVGG
                     KLHGAGANAFYEGIDDNGNMVRVPASAIAAPPTSAASAQNYAMKKDIDAIANADASAL
                     DFMTGMTGGAGNPAIGADVRSRLTGKEQRQLYNSAQRIQGRMQNQGVAAARDMGASGI
                     NTIAEAKMYFQGMPQVDYSSPEAMQQSIREIQEYTNNYNQQYNVNVGNGGLKSPRQQP
                     DTQQSAGGSYTSKSGIKFTVE"
BASE COUNT      409 a    324 c    411 g    310 t
ORIGIN      
        1 tgcttggctc gttgttttaa ggggtaatca atggctacgt ggcagcaggg cattaattca
       61 ggtggttttc tggctggcat tggtgcgcaa aatgagaacg caccaaaggc aagagatatt
      121 aacgcaacgc tgggtctgat tcgcgaaaac aatgatttag cccgttcagg cgctaataat
      181 gtggctttaa cagggctgcg tggtctggct ggcgttgctg atatttataa ccaggaacag
      241 caacagaaag cgctaaacgc attcaaccag gttcatgcca acgcatgggc tactggtgac
      301 ccgtctggcc tgtttaagtt tgctcaggaa aaccctgcgt ttgttgcgca ggcacagcag
      361 gcgttttccg gtcttaatga gcagcagcgt aacgatatgg gcgatttagc tatgaaggct
      421 aacgtcgctc tttctcaggg gccggaagcc tacagtaaat tcattactga taacaaggac
      481 aggttaaatc gtgttggcgc taatccggac tggatgatac agactggagt gcagaatcca
      541 gaacagctat cacacatgct gactacgatg tccctcggtg cgcttggacc cgaaaaggcg
      601 tttgctgttc aggataagat ggttggccgt gagattgacc gaggcaggct ggctgaaaca
      661 atccgcagca ataaagccgg tgaggggctt caggctcgcg ggcagaatat aacaatgcgt
      721 ggacaagata tgtcagcggc aacagcacga cgcggtcaag atttggcaac gcaaagagca
      781 aacgccagaa cgatatcagg cagcgaagga aatcgggtcg ttcagcttgc agacgggcga
      841 acagtcagcg tcggtggaaa acttcacggc gcaggggcga atgcgtttta cgaaggtatt
      901 gacgataacg gcaatatggt tcgtgtcccg gcaagcgcca ttgccgcacc tccaacgtct
      961 gcggcaagcg cacagaacta cgcaatgaag aaagacattg atgcaatcgc aaatgcagat
     1021 gcttctgctc tcgatttcat gactggaatg actggcggag caggaaatcc ggcaattggt
     1081 gcagatgttc gcagccgact cacaggcaaa gagcaacgcc agttatataa ctccgcacaa
     1141 cgtattcagg gaagaatgca gaatcagggc gtggcagcag caagagatat gggcgctagc
     1201 ggtatcaaca ccattgcaga agcgaagatg tattttcagg ggatgccgca ggttgactac
     1261 tcaagcccgg aggctatgca gcagtctatt cgtgagattc aggaatacac caacaattat
     1321 aaccagcagt acaacgttaa tgttggtaat ggtgggctga aatcaccaag gcagcagcca
     1381 gatactcagc aatcagccgg aggaagttac acgtctaaat ctggcattaa attcacggtg
     1441 gaataatgaa agtt
//