From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Zymonomas mobilis glucose-fructose oxidoreductase (gfor) gene,
Date: 25 Jul 92 10:07:42 GMT
Distribution: bionet
Checksum: 02878     5

LOCUS       ZMOGFOR      1666 bp ds-DNA             BCT       25-JUL-1992
DEFINITION  Zymonomas mobilis glucose-fructose oxidoreductase (gfor) gene,
            complete cds.
ACCESSION   M97379
KEYWORDS    glucose-fructose oxidoreductase.
SOURCE      Zymomonas mobilis (strain ZM6) (library: ATCC 29191) DNA.
  ORGANISM  Zymomonas mobilis
            Prokaryotae; Facultatively anaerobic gram-negative rods.
REFERENCE   1  (bases 1 to 1666)
  AUTHORS   Kanagasundaram,V. and Scopes,R.K.
  TITLE     Cloning, sequence analysis, and expression of the structrural gene
            encoding glucose-fructose oxidoreductase from Zymomonas mobilis
  JOURNAL   J. Bacteriol. 174, 1439-1447 (1992)
  STANDARD  simple staff_entry
FEATURES             Location/Qualifiers
     -35_signal      32..44
                     /gene="gfor"
     -10_signal      58..66
                     /gene="gfor"
     -35_signal      93..105
                     /gene="gfor"
     -10_signal      121..127
                     /gene="gfor"
     RBS             240..244
                     /gene="gfor"
     CDS             253..1572
                     /product="glucose-fructose oxidoreductase"
                     /gene="gfor"
                     /codon_start=1
                     /translation="MTNKISSSDNLSNAVSATDDNASRTPNLNRRALVGGGVGLAARG
                     ALASGLQAATLPAGASLVPTTPAGRPMPYAIRPMPEDRRFGYAIVGLGKYALNQILPG
                     FAGCQHSRMKLWSAVTEKAKIVAAEYGVDPRKIYDYSNFDKIAKDPKIDAVYIILPNS
                     LHAEFAIRSFKAGKHVMCEKPMATSVADCQRMIDAAKAANKKLMIGYRCHYDPMNRAA
                     VKLIRENQLGKLGMVTTDNSDVMDQNDLHSSGVCVVNSGGGSLMDIGIYGLNGTRYLL
                     GEEPIEVRAYTYSDPNDERFVEVEDRIIWQMRFRSGALSHGASSYSTTTTSRFSVQGD
                     KAVLLMDPATGYYQNLISVQTPGHANQSMMPQFIMPANNQFSAQLDHLAEAVINNKPV
                     RSPGEEGMQDVRLIQAIYEAARTGRAVNTDWGYVRQVVIDSDLTYLG"
     sig_peptide     253..408
                     /gene="gfor"
                     /codon_start=1
     terminator      1608..1644
                     /gene="gfor"
BASE COUNT      428 a    391 c    396 g    451 t
ORIGIN
        1 cagaaataat tatctgacag cgcttaccaa tcaattattg ccgaacgcag agtcccgtat
       61 taggacggtc aacaatctaa accgtttttc agaaaataat tgctttataa gcctcaaaac
      121 ttaaaagctg cggtatttta atataccaaa attttctgga aaagccggcg aatcagataa
      181 cagttccgca caggtgagaa ccacgacgga tcttctctga attgttggtt agttaagaaa
      241 gaaacaagga ttatgacgaa caaaatctcg tcttcagata atctttccaa tgctgtttca
      301 gcaacggatg acaacgcttc ccgtacgcca aatctgaacc gtcgcgctct cgttggtggt
      361 ggtgttggac tggccgcacg tggcgcctta gccagtggtc ttcaggcagc gacgcttcct
      421 gctggtgcca gcctggttcc gaccacgcct gcaggtcgcc cgatgcctta cgcgatccgc
      481 ccgatgccgg aagatcgtcg tttcggttat gctatcgtcg gtctgggtaa atatgccctt
      541 aaccagattt taccgggttt tgccggatgc cagcattccc gcatgaagct ttggtcagcg
      601 gtaaccgaaa aagctaaaat cgttgccgct gaatatggcg tcgatccccg taaaatttat
      661 gattacagca acttcgacaa gatcgctaaa gatccaaaaa tcgacgctgt ttacatcatt
      721 ttgccaaact ctttgcatgc tgaatttgct atccgttctt tcaaagccgg caagcatgtt
      781 atgtgtgaaa agccgatggc aacctctgtt gctgattgtc agcggatgat cgatgcagcc
      841 aaggctgcta ataaaaagct gatgatcggt taccgttgcc actatgatcc aatgaaccgt
      901 gcagcggtaa aattgatccg tgaaaaccag ttgggtaaac tgggcatggt taccaccgac
      961 aactcagacg ttatggatca gaacgacctg cacagcagtg gcgtctgcgt cgtgaactcc
     1021 ggtggcggtt ctttgatgga tatcggtatt tatggcttga acggtacccg ttacttgctg
     1081 ggtgaagaac cgatcgaagt ccgtgcttac acctacagcg atccgaatga tgaacgtttc
     1141 gttgaagtcg aagatcgtat tatttggcag atgcgcttca gaagcggtgc tctgtctcat
     1201 ggtgcatctt cttattcgac cacgacgact tcacgtttct cggtgcaggg cgacaaagct
     1261 gttctgttga tggatccggc taccggatat tatcagaatt tgatttctgt ccagacccca
     1321 ggccatgcta accagtcgat gatgccacag ttcatcatgc cagcgaacaa ccagttctct
     1381 gcacagttgg atcatctggc tgaagccgtc atcaataaca aaccagttcg tagcccgggt
     1441 gaagaaggta tgcaggatgt gcgcctgatt caggccattt atgaagcagc tcgtaccggt
     1501 cgcgccgtca acacggattg gggttatgtg cgtcaggtgg ttattgattc tgacttaacc
     1561 tatttgggtt aaacagactt atttttcctg ttttaggaaa atagttaaaa aggcgtcatt
     1621 ggttcttcca atgacgcctt tttttataaa caaaaaaatc ctttgt
//