From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Plasmid F (from E.coli) trbF, traH, and traG genes, complete cds,
Date: 9 Apr 92 10:02:56 GMT
Distribution: bionet
Checksum: 61691    11

LOCUS       FPLTRAH      4847 bp ds-DNA             BCT       09-APR-1992
DEFINITION  Plasmid F (from E.coli) trbF, traH, and traG genes, complete cds,
            and traS gene, 5' end.
ACCESSION   M59763
KEYWORDS    F pilus assembly.
SOURCE      Plasmid F DNA.
  ORGANISM  Plasmid F
            Prokaryota; Bacteria.
REFERENCE   1  (bases 1 to 4847)
  AUTHORS   Ham,L.M., Firth,N. and Skurray,R.
  TITLE     Nucleotide sequence of the F plasmid transfer gene, traH:
            identification of a new gene and a promoter within the transfer
            operon
  JOURNAL   Gene 75, 157-165 (1989)
  STANDARD  simple staff_entry
REFERENCE   2  (bases 1 to 4847)
  AUTHORS   Firth,N. and Skurray,R.A.
  TITLE     Characterization of the F plasmid bifunctional conjugation gene,
            traG
  JOURNAL   Mol. Gen. Genet. 232, 145-153 (1992)
  STANDARD  simple staff_entry
COMMENT     Base pairs 1 to 1671 appear in Gene, volume 75, pages 157-165,
            whereas base pairs 1666 to 4847 are in preparation.
FEATURES             Location/Qualifiers
     CDS             173..553
                     /gene="trbF"
                     /codon_start=1
                     /translation="MRENKSNPELKIRSTERDYKYISRITGRYAGLSLVFLTAGIVLW
                     TVMDIIFDACIDSWKADPELNNSSYMWNILIYAIPYTLYALAAGFLVTFFSVPNVRIN
                     IRKYRDIPAEMSYAPGEHIKGGQE"
     CDS             550..1926
                     /gene="traH"
                     /evidence=EXPERIMENTAL
                     /codon_start=1
                     /translation="MMPRIKPLLVLCAALLTVTPAASADVNSDMNQFFNKLGFASNTT
                     QPGVWQGQAAGYAYGGSLYARTQVKNVQLISMTLPDINAGCGGIDAYLGSFSFINGEQ
                     LQRFVKQIMSNAAGYFFDLALQTTVPEIKTAKDFLQKMASDINSMNLSSCQAAQGIIG
                     GLFPRTQVSQQKVCQDIAGESNIFADWAASRQGCTVGGKSDSVRDKASDKDKERVTKN
                     INIMWNALSKNRMFDGNKELKEFVMTLTGSLVFGPNGEITPLSARTTDRSIIRAMMEG
                     GTAKISHCNDSDKCLKVVADTPVTISRDNALKSQITKLLASIQNKAVSDTPLDDKEKG
                     FISSTTIPVFKYLVDPQMLGVSNSMIYQLTDYIGYDILLQYIQELIQQARAMVATGNY
                     DEAVIGHINDNMNDATRQIAAFQSQVQVQQDALLVVDRQMSYMRQQLSARMLSRYQNN
                     YHFGGSTL"
     sig_peptide     550..621
                     /gene="traH"
                     /evidence=EXPERIMENTAL
                     /codon_start=1
     mat_peptide     622..1923
                     /gene="traH"
                     /evidence=EXPERIMENTAL
                     /codon_start=1
     CDS             1923..4739
                     /gene="traG"
                     /codon_start=1
                     /translation="VNEVYVIAGGEWLRNNLNAIAAFMGTWTWDSIEKIALTLSVLAV
                     AVMWVQRHNVMDLLGWVAVFVLISLLVNVRTSVQIIDNSDLVKVHRVDNVPVGLAMPL
                     SLTTRIGHAMVASYEMIFTQPDSVTYSKTGMLFGANLIVKSTDFLSRNPEIINLFQDY
                     VQNCVLGDIYLNHKYTLEDLMASADPYTLIFSRPSPLRGVYDNNNNFITCKDASVTLK
                     DRLNLDTKTGGKTWHYYVQQIFGGRPDPDLLFRQLVSDSYSYFYGSSQSASQIMRQNV
                     TMNALKEGITSNAARNGDTASLVSLATTSSMEKQRLAHVSIGHVTMRNLPMVQTILTG
                     IAIGIFPLLILAAVFNKLTLSVLKGYVFALMWLQTWPLLYAILNSAMTFYAKQNGAPV
                     VLSELSQIQLKYSNLASTAGYLSAMIPPLSWMMVKGLGAGFSSVYSHFASSSISPTAS
                     AAGSVVDGNYSYGNMQTENVNGFSWSTNSTTSFGQMMYQTGSGATATQTRDGNMVMDA
                     SGAMSRLPVGINATRQIAAAQQEMAREASNRAESALHGFSSSIASAWNTLSQFGSNRG
                     SSDSVTGGADSTMSAQDSMMASRMRSAVESYAKAHNISNEQATRELASRSTNASLGLY
                     GDAYAKGHLGISVLGNGGGVGLQAGAKASIDGSDLDSHEASSGSRASHDARHDIDARA
                     TQDFKEASDYFTSRKVSESGSHTDNNADSRVDQLSAALNSAKQSYDQYTTNMTRSHEY
                     AEMASRTESMSGQMSEDLSQQFAQYVMKNAPQDVEAILTNTSSPEIAERRRAMAWSFV
                     QEQVQPGVDNTWRESRRDIGKGMESVPSGGGSQDIIADHQGHQAIIEQRTQDSNIRND
                     VKHQVDNMVTEYRGNIGDTQNSIRGEENIVKGQYSELQNHHKTEALTQNNKYNEEKLA
                     QERIPGADSPKELLEKAKSYQHKE"
                     /partial
     CDS             4772..>4847
                     /gene="traS"
                     /codon_start=1
                     /translation="MITQQIISSELEVLKKHIDSGDIRI"
BASE COUNT     1289 a   1099 c   1283 g   1176 t
ORIGIN
        1 cttttttatc ccgccagccc tgtagaaatt gtcgtttcgc tgagtcttac cttttctgtt
       61 tctgttgtgt tttttgtggg ggcaattgtg ggatggattt cggtgtctga aatatattac
      121 agccgcatga ccggtctgaa tgaatcatcg gaaatatcag agggaactta taatgagaga
      181 aaataaaagt aatcctgaac tcaaaatcag aagtacagaa cgagattata aatacatttc
      241 cagaattact ggcaggtatg ctggattgtc tctggtattc ctgactgccg gaattgtttt
      301 atggacagtt atggatatta tttttgatgc ctgtattgat tcgtggaaag cggacccgga
      361 attaaataat tcgagttata tgtggaatat cctgatatat gccattccgt atacgttata
      421 tgcgcttgca gccgggtttc ttgttacatt tttttctgtc cccaatgtac gaattaatat
      481 acggaaatat cgagatattc ctgctgaaat gtcgtatgca ccgggagaac acattaaggg
      541 agggcaagaa tgatgccacg cattaaacct cttctggttc tttgtgccgc cttactgacg
      601 gtgacgcctg cagcgtcagc ggatgtgaac agcgacatga atcagttctt taataagctg
      661 ggatttgctt ccaataccac acagccgggc gtctggcagg ggcaggccgc cggctatgct
      721 tacggtggct ccctgtatgc ccgtactcag gtcaaaaatg ttcagctaat ttccatgacg
      781 ctgccggata tcaatgctgg atgtggcggc atcgacgcct atctcggctc gttcagtttt
      841 attaatggtg agcagttgca gcgttttgtt aagcagatta tgagcaatgc tgccggttac
      901 ttttttgacc ttgccctgca gacaacggtg ccggaaataa aaaccgcaaa agacttcctg
      961 cagaaaatgg caagcgacat taacagtatg aacctcagtt cctgccaggc tgcacagggg
     1021 attatcggcg ggcttttccc ccggacgcag gtgtcccagc aaaaagtctg tcaggacatt
     1081 gccggtgaga gcaatatttt tgctgactgg gcggcttccc ggcagggatg taccgttggc
     1141 gggaaatctg acagcgtcag ggataaagcc agcgacaagg ataaggagcg ggtgaccaaa
     1201 aacatcaaca tcatgtggaa tgcgctttcc aaaaacagaa tgtttgacgg caacaaagag
     1261 ctgaaagagt ttgtgatgac gctgaccggc tcactggtgt ttggtcccaa cggcgaaatc
     1321 acaccgctgt cagccagaac cactgaccgt tcaattatcc gggccatgat ggaaggcggc
     1381 acagcaaaaa tttctcactg caacgattct gataaatgcc tgaaagtggt ggcagacaca
     1441 ccggtgacca tcagccggga taatgcactg aagtctcaga ttactaaact tctggccagc
     1501 attcagaaca aggctgtcag tgacacgcct ctggatgaca aggaaaaagg ctttatttcc
     1561 agtaccacca tccccgtctt caaatacctg gttgacccgc agatgctcgg tgtttccaac
     1621 agtatgattt accagctgac ggactatatc ggttacgaca tcctgctgca gtacattcag
     1681 gagctgatac agcaggcacg ggcgatggtg gccacgggaa attatgacga agcggttatc
     1741 gggcatatta acgacaacat gaatgatgcc acccggcaga ttgcggcgtt tcagtcacag
     1801 gtgcaggtac agcaggatgc gctgctggtt gtcgatcgtc agatgagcta catgcgtcag
     1861 cagctttccg cccgcatgct cagtcgttac cagaacaact atcacttcgg agggagcacg
     1921 ctgtgaatga agtttatgtg attgccggtg gtgagtggtt gcggaataac ctgaacgcca
     1981 ttgccgcctt tatgggaacc tggacgtggg attccattga aaaaattgcg ctcacattgt
     2041 ctgttctcgc ggtggccgta atgtgggtac agcggcacaa cgtgatggat ttgctgggct
     2101 gggtggccgt gtttgtgctt atcagcctgc tggttaatgt ccgcacatcg gtgcagatta
     2161 ttgataacag tgacctggtc aaagttcacc gggtggataa tgtgccggtc ggtctggcga
     2221 tgccactttc actgacgacc cgtatcgggc atgcaatggt ggccagttac gagatgatct
     2281 tcacgcaacc ggacagcgtc acctacagca aaacggggat gctgttcggg gcgaatctga
     2341 ttgtgaaaag caccgatttc ctgtcccgga atccggaaat catcaatctg ttccaggact
     2401 acgtccagaa ctgcgtgctg ggtgatattt acctgaatca taaatacacg ctggaagacc
     2461 tgatggcctc cgctgacccc tacacgctga ttttttcccg ccccagcccg ctacggggcg
     2521 tttatgacaa caataataat ttcataactt gtaaggatgc gtcggtcacg ctgaaagaca
     2581 ggctgaatct cgatacaaag acgggaggca agacctggca ttattatgtg cagcagatat
     2641 ttggcggcag accggacccg gacctgttat tcagacaact ggtcagtgac agttacagtt
     2701 atttctacgg ctccagtcag tctgccagcc agattatgcg ccagaacgtc accatgaatg
     2761 ccctgaaaga gggtatcacc agtaatgcag cccgtaacgg tgacaccgcc agcctggtaa
     2821 gtctggccac cacgtcatcg atggagaaac aacgtctggc acatgtctcc attggtcatg
     2881 taactatgcg aaacctgccg atggtccaga ctatcctgac ggggattgcc attggtatat
     2941 tcccgctcct gatactggcg gctgtcttta acaagctgac attgtctgta ttaaaggggt
     3001 atgtgtttgc cctgatgtgg ctgcagacct ggccgttgtt gtatgccatt ctgaacagtg
     3061 ccatgacatt ctatgcgaaa cagaatggtg cgccggtcgt gttgtctgaa ctctctcaga
     3121 tacagctgaa atactctaac ctggcctcca ctgccgggta tctttcagcc atgatccccc
     3181 cgttgtcatg gatgatggta aaaggccttg gcgcaggttt ttccagcgtg tacagccact
     3241 tcgcctcttc atctatcagt ccaacggcca gtgcagcagg cagtgtggtt gacggtaatt
     3301 actcctacgg caacatgcag acggaaaacg tgaacggctt cagctggagc accaacagca
     3361 ccacgtcgtt tggtcagatg atgtaccaga ccggcagtgg cgcaaccgcc acacagaccc
     3421 gtgacggtaa tatggtgatg gatgcaagcg gagcgatgtc ccggttaccg gtcggtatca
     3481 atgcaacgcg tcagattgca gcggcacaac aggaaatggc ccgggaggcg tcgaacagag
     3541 ccgaaagtgc cctgcatggt ttcagcagca gtattgccag tgcctggaac acactcagtc
     3601 agtttggctc taaccggggg agcagtgact ctgtcactgg tggtgctgac agcacgatga
     3661 gtgctcagga ctccatgatg gccagccgta tgcgcagtgc agtggaaagc tatgcgaagg
     3721 cgcataatat cagtaatgag caggcgacga gagaactggc ctcgagaagt acaaatgcgt
     3781 cgcttggcct ttacggtgat gcttatgcta aagggcattt aggcataagt gtattgggta
     3841 acggtggagg ggttggtctc caggccggag ctaaggcaag cattgatggc agtgatcttg
     3901 actcacatga agccagtagt ggttcacggg ccagccatga tgctcgtcat gatatcgatg
     3961 ccagagctac acaagacttt aaagaagcca gcgattactt taccagccgc aaagtcagtg
     4021 aatccggcag ccatactgac aataatgctg attcccgtgt ggatcagttg tctgcagccc
     4081 tgaattcagc gaaacagagt tacgaccagt acacgacgaa tatgacccgc agccatgaat
     4141 atgctgaaat ggcatcccgt actgagagta tgagtgggca gatgagtgaa gatctgtcgc
     4201 aacagtttgc acagtatgtg atgaaaaacg caccgcagga tgttgaagct attctgacaa
     4261 ataccagttc gccggagatt gcagaacgcc gtcgggctat ggcgtggtct tttgtgcagg
     4321 aacaggtaca gcctggtgtt gataacacct ggcgtgaatc ccgtagagat ataggtaaag
     4381 gaatggagag cgtaccttcg ggtggtggca gccaggatat tattgctgat catcagggac
     4441 atcaggccat tattgagcaa agaacgcagg acagtaatat ccgtaatgat gtaaaacatc
     4501 aggttgataa tatggtcaca gaatatcgag gtaatatcgg agatactcag aacagcatcc
     4561 gaggcgagga aaatattgta aaagggcagt attctgaact acaaaatcat cataaaacag
     4621 aagcattaac tcagaataat aaatataatg aagaaaagct ggctcaggaa agaatacctg
     4681 gtgctgatag tccgaaagag cttttagaga aagcaaagag ttaccagcat aaagaataat
     4741 cataacatgt tgggtagggt atggagagac catgattaca caacaaatta tcagtagtga
     4801 gttggaagtt ttaaagaaac atattgactc aggagatatt agaattc
//