From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Lactococcus lactis replication protein gene, complete cds.
Date: 29 Sep 92 10:07:19 GMT
Distribution: bionet
Checksum: 12073     5

LOCUS       LACREPPRO    2359 bp ds-DNA             BCT       29-SEP-1992
DEFINITION  Lactococcus lactis replication protein gene, complete cds.
ACCESSION   L02920
KEYWORDS    replication protein.
SOURCE      Lactococcus lactis DNA.
  ORGANISM  Lactococcus lactis
            Prokaryotae; Firmicutes; Firmibacteria; Gram-positive cocci;
            Streptococcaceae.
REFERENCE   1  (bases 1 to 2359)
  AUTHORS   von Wright,A. and Raty,K.
  TITLE     The nucleotide sequence of the replication region of pVS40, a
            lactococcal food grade cloning vector
  JOURNAL   Unpublished (1992)
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     repeat_region   537..621
                     /rpt_type=tandem
                     /note="putative"
                     /citation=[1]
                     /partial
     -35_signal      621..626
     -10_signal      644..649
     stem_loop       654..691
     RBS             710..716
     CDS             726..1925
                     /product="replication protein"
                     /note="putative"
                     /codon_start=1
                     /translation="MSIIPEKQENQKQVLTLNELSKRKVVEHNSLITSIAKMDKTPLK
                     MFELAVSCINTEEPPKDNTVYLSKRDLFAFFKVSDNDKHSRFKQAVEKMQKQAFFQIK
                     EEQGKGFKFKSIVPIPYVEWTDYNDEVKIEFHREIMPYLINLKKNFTQHALSDIAELN
                     SKYSIILYRWLSMQYNQYEHYSVKGGRRAEQVEAYRNPSISIKELRIITDTVNSYKQF
                     TRFNNDVLKTPLDEINAHTSFNVTYDKIKKGRSIDSIVFHIEKKRMADDNSYKLDDRA
                     YQEDKARKAETEDQLVLQAMDSPYTKLLMEHFLLSYLDLMDKKILAGLQKNVYPLYDE
                     LKDLRGLNGVKDHLSYVASKKEAYSKRNVAKYLKKAIEQYLPTVKLQDLEQPERAKVR
                     GKGASHE"
                     /citation=[1]
BASE COUNT      903 a    376 c    413 g    667 t
ORIGIN
        1 cgatttttaa aatttgcaac ggaaccaaaa gtagatcata acttaacagt tttgacctat
       61 ttttagcatg cagatgaata gtgagattag agcaggtcat aaataagggt catatttaaa
      121 agctaaaatt gtacgtttta gtcaattaca tacaagccta tttaagggct ttttctttta
      181 tccatgataa ttattactaa ctcacgttta aaatcgctta taatgcaaac tatgagcttg
      241 taggactatt taaagcgttt agactaaaca aagtagccac gaccgttagg gagttctttt
      301 tagtgtcaga atgtatctaa tataaaatta gagtgtacaa gcggagcgac aagagcaacg
      361 cagggagttt aaatttagaa cctcaattca atggttttga ctttttcttt tgaccttgat
      421 tttaattttt gaaaaaaata aaaaaaggcg aagcctatat taatttatca tatatatttt
      481 aatcttttat tcttttgcgt gggaaaaaag tcagtgttta aaggtggata cagaattata
      541 gcgtatgaaa aaactgtgta tagcgtatga aaaaactgtg tatagcgtat gaaaaaactg
      601 tgtatagcgt atgaaaaaac ttgatagata tacggtattc tgatacaata aaagcataga
      661 gaaatcgaca gcgaaatcag tttctctatg cctacttaaa atcactcaca aaggagtaac
      721 tttctatgtc tattatacca gaaaaacaag aaaaccaaaa gcaggtgctg accttgaatg
      781 aactttcaaa aagaaaagtg gtagagcata acagcttgat aacctctatt gctaaaatgg
      841 ataaaacgcc tttaaaaatg tttgagttag ccgtgtcttg tatcaatacc gaagagccac
      901 cgaaagacaa tacggtttat ctttcaaaaa gagacctttt cgcttttttt aaagtttccg
      961 acaatgataa gcatagtcgc tttaaacaag cggttgagaa aatgcaaaaa caagcttttt
     1021 ttcaaatcaa agaagaacaa ggaaagggtt ttaaattcaa aagcattgtg ccgattcctt
     1081 acgtagaatg gacggattat aacgacgaag taaaaattga atttcatcgt gaaattatgc
     1141 cttacttaat caatctcaaa aagaatttta cccaacacgc tttatcagat atagccgaac
     1201 tcaatagcaa gtattcaatt attctctacc gttggttatc tatgcaatac aaccaatacg
     1261 agcattacag tgtcaaaggc ggacggcgag ccgaacaagt ggaagcatac cgcaatccgt
     1321 ctatttcaat taaagagttg cgtatcataa ctgataccgt aaattcttat aaacagttta
     1381 caagatttaa caatgatgtt cttaaaacac ctttagatga aattaacgcc catacgtctt
     1441 ttaatgtgac ctatgacaaa atcaaaaaag ggcgttcaat tgattctatt gtctttcaca
     1501 ttgaaaagaa acgcatggca gacgataaca gttacaagtt ggacgatagg gcttatcaag
     1561 aagataaagc acgtaaagct gaaacagaag accaattagt cttgcaggca atggatagcc
     1621 cttatactaa gttacttatg gaacatttct tattgtctta tcttgacctt atggacaaaa
     1681 agatattggc aggcttacag aaaaacgttt atccgcttta tgacgaactc aaagacttac
     1741 gaggattaaa tggcgtcaaa gaccacttgt cttatgttgc cagcaaaaaa gaagcctatt
     1801 ctaaacgtaa tgtagcgaaa tatcttaaaa aagccattga acaataccta ccaactgtaa
     1861 aattacaaga ccttgaacaa ccagaacgtg caaaggttcg aggtaaaggt gcaagccatg
     1921 agtgagaatt taaaaacgat taaggagctt gcggatgagt taggtgtaag taaatcatat
     1981 attgataaaa taatacgcat actggaattg catactaaat tagataaagt aggtaataag
     2041 tatgtgattt ctaaaaaaca agagaaatct ataaaagata gacttaggtc gtctgaatca
     2101 acaacaaaat cacacactaa atcgacaact aaaacgcata ctgaagttga ttttgaagtc
     2161 gattttttga aagaagaaat cttatatctt agaaagagtc acgataaaca attgaccaac
     2221 aaagataagc agatagaaac tttaagtaat cttttagatc aacaacaacg tctagccttg
     2281 caagataaaa atttgctaga agaatacaaa gcagaaaatg acagattaaa agttctcaaa
     2341 atgccctcac aggaaacag
//