From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Escherichia coli (clone pN309) acyl-CoA synthetase (fadD) mRNA,
Date: 22 Dec 92 22:51:08 GMT
Distribution: bionet
Checksum: 01387 5

LOCUS       ECOFADD      2230 bp ds-DNA             BCT       30-NOV-1992
DEFINITION  Escherichia coli (clone pN309) acyl-CoA synthetase (fadD) mRNA,
            complete cds.
ACCESSION   L02649
KEYWORDS    acyl-CoA synthetase; fadD gene.
SOURCE      Escherichia coli (strain K-12) DNA.
  ORGANISM  Escherichia coli
            Prokaryotae; Gram-negative facultatively anaerobic rods;
            Enterobacteriaceae.
REFERENCE   1  (bases 1 to 2230)
  AUTHORS   Black,P.N., DiRusso,C.C., Metzger,A.K. and Heimert,T.L.
  TITLE     Cloning, sequencing, and expression of the fadD gene of Escherichia
            coli encoding acyl coenzymeA synthetase
  JOURNAL   J. Biol. Chem. (1993) In press
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     protein_bind    122..138
                     /function="FadR protein binding site OD2"
                     /bound_moiety="FadR protein"
                     /gene="fadD"
     protein_bind    218..234
                     /function="FadR protein binding site OD1"
                     /bound_moiety="FadR protein"
                     /gene="fadD"
     CDS             307..1983
                     /EC_number="6.2.1.3"
                     /transl_except=(pos:307..309,aa:Met)
                     /gene="fadD"
                     /product="acyl-CoA synthetase"
                     /codon_start=1
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVGALRRSTCVCE
                     YGGGNDLRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPTRLKMSSCSMVAYRKSRLLAYLPGSSGEGVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARQSGQ"
BASE COUNT      542 a    501 c    615 g    572 t
ORIGIN      
        1 gatccatggt tttatggcgg tcgtggctgg ccctatggct acggcggatg gggctggtat
       61 aatcccggcc ccgcgagagt acaaacagtt gtaactgaat aattgcttgt ttttaaagaa
      121 aaagaaacag cggctggtcc gctgtttctg cattcttacg gtaaagataa aaataaatag
      181 tgacgcgctt cgcaaccttt tcgttgggta attatcaagc tggtatgatg agttaatatt
      241 atgttaacgg catgtatatc atttggggtt gcgatgacga cgaacacgca ttttagaggt
      301 gaagaattga agaaggtttg gcttaaccgt tatcccgcgg acgttccgac ggagatcaac
      361 cctgaccgtt atcaatctct ggtagatatg tttgagcagt cggtcggcgc gctacgccga
      421 tcaacctgcg tttgtgaata tgggggaggt aatgaccttc gcaagctgga agaacgcagt
      481 cgcgcgtttg ccgcttattt gcaacaaggg ttggggctga agaaaggcga tcgcgttgcg
      541 ttgatgatgc ctaatttatt gcaatatccg gtggcgctgt ttggcatttt gcgtgccggg
      601 atgatcgtcg taaacgttaa cccgttgtat accccgcgtg agcttgagca tcagcttaac
      661 gatagcggcg catcggcgat tgttatcgtg tctaactttg ctcacacact ggaaaaagtg
      721 gttgataaaa ccgccgttca gcacgtaatt ctgacccgta tgggcgatca gctatctacg
      781 gcaaaaggca cggtagtcaa tttcgttgtt aaatacatca agcgtttggt gccgaaatac
      841 catctgccag atgccatttc atttcgtagc gcactgcata acggctaccg gatgcagtac
      901 gtcaaacccg aactggtgcc ggaagattta gcttttctgc aatacaccgg cggcaccact
      961 ggtgtggcga aaggcgcgat gctgactcac cgcaatatgc tggcgaacct ggaacaggtt
     1021 aacgcgacct atggtccgct gttgcatccg ggcaaagagc tggtggtgac ggcgctgccg
     1081 ctgtatcaca tttttgccct gaccattaac tgcctgctgt ttatcgaact gggtgggcag
     1141 aacctgctta tcactaaccc gcgcgatatt ccagggttgg taaaagagtt agcgaaatat
     1201 ccgtttaccg ctatcacggg cgttaacacc ttgttcaatg cgttgctgaa caataaagag
     1261 ttccagcagc tggatttctc cagtctgcat ctttccgcag gcggtgggat gccagtgcag
     1321 caagtggtgg cagagcgttg ggtgaaactg accggacagt atctgctgga aggctatggc
     1381 cttaccgagt gtgcgccgct ggtcagcgtt aacccatatg atattgatta tcatagtggt
     1441 agcatcggtt tgccggtgcc gtcgacggaa gccaaactgg tggatgatga tgataatgaa
     1501 gtaccaccag gtcaaccggg tgagctttgt gtcaaaggac cgcaggtgat gctgggttac
     1561 tggcagcgtc ccgatgctac cgatgaaatc atcaaaaatg gctggttaca caccggcgac
     1621 atcgcggtaa tggatgaaga aggattcctg cgcattgtcg atcgtaaaaa agacatgatt
     1681 ctggtttccg gttttaacgt ctatccaacg agattgaaga tgtcgtcatg cagcatggtg
     1741 gcgtacagga agtcgcggct gttggcgtac cttcctggct ccagtggtga aggcgtgaaa
     1801 atcttcgtag tgaaaaaaga tccatcgctt accgaagagt cactggtgac tttttgccgc
     1861 cgtcagctca cgggatacaa agtaccgaag ctggtggagt ttcgtgatga gttaccgaaa
     1921 tctaacgtcg gaaaaatttt gcgacgagaa ttacgtgacg aagcgcggca aagtggacaa
     1981 taaagcctga gcgttaagtc agtcgtcaga cgccggttaa tccggcgttt tttttgacgc
     2041 ccactaaaga gaaaacaatt tgaattacca aatgattacc acggacgatg cgctggcttc
     2101 tttgtgtgaa gccgtccgtg cctttccggc gatagccctg gatactgaat ttgttcgtac
     2161 gcgcacttat tacccgcagc cggggttgat tcaacttttc gatggcgagc atctggcgct
     2221 aatcggatcc
//