From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Drosophila melanogaster pumilio protein mRNA, complete cds.
Date: 7 Jan 93 05:46:11 GMT
Distribution: bionet
Checksum: 41536 16

LOCUS       DROPUM       6833 bp ss-mRNA            INV       06-JAN-1993
DEFINITION  Drosophila melanogaster pumilio protein mRNA, complete cds.
ACCESSION   L07943
KEYWORDS    pumilio protein.
SOURCE      Drosophila melanogaster 0 to 8 hours embryo cDNA to mRNA.
  ORGANISM  Drosophila melanogaster
            Eukaryota; Animalia; Metazoa; Arthropoda; Uniramia; Insecta;
            Pterygota; Neoptera; Holometabola; Diptera; Brachycera;
            Cyclorrhapha; Schizophora; Drosophiloidea; Drosophilidae.
REFERENCE   1  (bases 1 to 6833)
  AUTHORS   Barker,D.D., Wang,C., Moore,J., Dickinson,L.K. and Lehmann,R.
  TITLE     Pumilio is essential for function but not for distribution of the
            Drosophila abdominal determinant Nanos
  JOURNAL   Genes Dev. 6, 2312-2326 (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     exon            1..240
                     /number=1
                     /gene="pumilio"
                     /evidence="EXPERIMENTAL"
     exon            241..1376
                     /number=2
                     /gene="pumilio"
                     /evidence="EXPERIMENTAL"
     misc_difference replace(1277,"g")
                     /evidence="EXPERIMENTAL"
                     /note="change observed in genomic DNA"
     exon            1377..2159
                     /number=3
                     /gene="pumilio"
                     /evidence="EXPERIMENTAL"
     exon            2160..2401
                     /number=4
                     /gene="pumilio"
                     /evidence="EXPERIMENTAL"
     exon            2402..2528
                     /number=5
                     /gene="pumilio"
                     /evidence="EXPERIMENTAL"
     exon            2529..2647
                     /number=6
                     /gene="pumilio"
                     /evidence="EXPERIMENTAL"
     exon            2648..2936
                     /number=7
                     /gene="pumilio"
                     /evidence="EXPERIMENTAL"
     exon            2937..3023
                     /number=8
                     /gene="pumilio"
                     /evidence="EXPERIMENTAL"
     misc_difference replace(2975,"c")
                     /evidence="EXPERIMENTAL"
                     /note="change observed in genomic DNA"
     exon            3024..3488
                     /number=9
                     /gene="pumilio"
                     /evidence="EXPERIMENTAL"
     exon            3489..3705
                     /number=10
                     /gene="pumilio"
                     /evidence="EXPERIMENTAL"
     exon            3706..4983
                     /number=11
                     /gene="pumilio"
                     /evidence="EXPERIMENTAL"
     misc_difference replace(4351,"g")
                     /evidence="EXPERIMENTAL"
                     /note="change observed in genomic DNA"
     exon            4984..5152
                     /number=12
                     /gene="pumilio"
                     /evidence="EXPERIMENTAL"
     exon            5153..6833
                     /number=13
                     /gene="pumilio"
                     /evidence="EXPERIMENTAL"
     misc_difference replace(5396,"g")
                     /evidence="EXPERIMENTAL"
                     /note="change observed in genomic DNA"
     misc_difference replace(5399,"a")
                     /evidence="EXPERIMENTAL"
                     /note="change observed in genomic DNA"
     misc_difference replace(5529,"g")
                     /evidence="EXPERIMENTAL"
                     /note="change observed in genomic DNA"
     misc_difference replace(5598,"a")
                     /evidence="EXPERIMENTAL"
                     /note="change observed in genomic DNA"
     misc_difference replace(5851..5852,"atag")
                     /evidence="EXPERIMENTAL"
                     /note="change (insertion) observed in genomic DNA"
     misc_difference replace(5878,"a")
                     /evidence="EXPERIMENTAL"
                     /note="change observed in genomic DNA"
     misc_difference replace(5986,"c")
                     /evidence="EXPERIMENTAL"
                     /note="change observed in genomic DNA"
     misc_difference replace(6053,"a")
                     /evidence="EXPERIMENTAL"
                     /note="change observed in genomic DNA"
     misc_difference replace(6058,"g")
                     /evidence="EXPERIMENTAL"
                     /note="change observed in genomic DNA"
     misc_difference replace(6175,"t")
                     /evidence="EXPERIMENTAL"
                     /note="change observed in genomic DNA"
     misc_difference replace(6466,"c")
                     /evidence="EXPERIMENTAL"
                     /note="change observed in genomic DNA"
     misc_difference replace(6726,"a")
                     /evidence="EXPERIMENTAL"
                     /note="change observed in genomic DNA"
     polyA_signal    6814..6819
                     /gene="pumilio"
                     /note="putative"
     CDS             1044..5645
                     /gene="pumilio"
                     /evidence="EXPERIMENTAL"
                     /note="maternally required for embryonic abdomen
                     formation"
                     /product="pumilio protein"
                     /codon_start=1
                     /translation="MKFLGGNDDRNGRGGVGVGTDAIVGSRGGVSQDAADAAGAAAAA
                     AVGYVFQQRPSPGGVGVGVGGVGGGVPGVGAVGSTLHEAAAAEYAAHFAQKQQQTRWA
                     CGDDGHGIDNPDKWKYNPPMNPANAAPGGPPGNGSNGGPGAIGTIGMGSGLGGGGGGG
                     AGGGNNGGSGTNGGLHHQSMAAAAANMAAMQQAAALAKHNHMISQAAAAVAAQQQHQH
                     PHQQHPQQQQQQQQAQNQGHPHHLMGGGNGLGNGNGLGIQHPGQQQQQQQQQQQQQHP
                     GQYNANLLNHAAALGHMSSYAQSGGSMYDHHGGAMHPGMNGGMPKQQPLGPPGAGGPQ
                     DYVYMGGQTTVPMGAAMMPPQNQYMNSSAVAAANRNAAITTSTAKKLWEKSDGKGVSS
                     STPGGPLHPLQIPGIGDPSSVWKDHTWSTQGENILVPPPSRAYAHGGASDTSNSGNAG
                     ILSPRDSTCAKVVEYVFSGSPTNKDSSLSGLEPHLRNLKFDDNDKSRDDKEKANSPFD
                     TNGLKKDDQVTNSNGVVNGIDDDKGFNRTPGSRQPSPAEESQPRPPNLLFPPLPFNHM
                     LMDHGQGMGGGLGGVVGSGNGVGGGSGGGGAGGAYAAHQQMAAQMSQLQPPMMNGVGG
                     GMPMAAQSPMLNHQAAGPNHMESPGNLLQQQNFDVQQLFRSQNPGLAAVATNAAAAAA
                     AAAAATSAASAAAAVGAPPVPNGSLQQSQQQQQQQQQQQQQQQMHMAAASQQFLAAQQ
                     QAQNAAYAAQQATSYVINPGQEAAPYMGMIAAAQMPYYGVAPWGMYPGNLIPQQGTQP
                     RRPLTPSQQGAENQPYQVIPAFLDHTGSLLMGGPRTGTPMRLVSPAPVLVPPGATRAG
                     PPPPQGPQLYQPQPQTAQQNLYSQQNGSSVGGLALNTSSLTGRRDSFDRSTSAFSPST
                     MDYTSSGVAAAANAVNSTVAQAAAAAAAAAAARGKWPGAMSGAASGAYGALGAGNASA
                     SPLGAPITPPPSAQSCLLGSRAPGAESRQRQQQQQQLAAVGLPATAAAAQAAVAAAAN
                     NMFGSNSSIFSNPLAIPGTAAVAAAAAAAAAANSRQVAATAAAAAAVAAAAGGVGGAP
                     QPGRSRLLEDFRNQPYPNLQLRDLANHIVEFSQDQHGSRFIQQKLERATAAEKQMVFS
                     EILAAAYSLMTDVFGNYVIQKFFEFGTPEQKNTLGMQVKGHVLQLALQMYGCRVIQKA
                     LESISPEQQQEIVHELDGHVLKCVKDQNGNHVVQKCIECVDPVALQFIINAFKGQVYS
                     LSTHPYGCRVIQRILEHCTAEQTTPILDELHEHTEQLIQDQYGNYVIQHVLEHGKQED
                     KSILINSVRGKVLVLSQHKFASNVVEKCVTHATRGERTGLIDEVCTFNDNALHVMMKD
                     QYANYVVQKMIDVSEPTQLKKLMTKIRPHMAALRKYTYGKHINAKLEKYYMKITNPIT
                     VGTGAGGVPAASSAAAVSSGATSASVTACTSGSSTTTTSTTNSLASPTICSVQENGSA
                     MIVEPSSPDASESSSSVVSGAVNSGLGPIGPPTNGNVVL"
BASE COUNT     1904 a   1835 c   1721 g   1373 t
ORIGIN      
        1 ctctcctctc tctctagctc tttgttgtag ttgtatttgg ccagtgtgtc tttgcatttc
       61 gcttttatta tcgttctgcg acggacggcg gcgacgttga agaaattcct ggccgatttc
      121 ttcttcatca tcctgtgtaa ataattgtaa tttaaataat ctaaatttcg tatttaacga
      181 aagcacacac accatcaaat caatggaaat gcaaacgaga aggctttttt aagtgcatgt
      241 tttgtgtata ttttcatcat taaaacgtcg tgtaaattaa aattcatcct aattataatt
      301 aataaactac attaataaac ggaaattcca caattctcaa caagaaatct gtaaaaattc
      361 aagttttaac caattctcat acgtaattaa ataaataaag ttcagttttg ttaagtccca
      421 aaaacaacag ctgtgcaact gtgtaaaata ttattcataa ttttgtgtaa atttatgtaa
      481 aaaaaaaaaa aaaaacaaac caaccacgac aaccaaacca aacgaagcaa attcaatttt
      541 tttgatcaaa aagtgtgtga aaaatctcag caaagcctgt aaaataaata ttaaatatat
      601 taagcaaaaa gtgtggtttc ttttacaaca aaataaataa aagcaaaaaa caagtgtaaa
      661 tctgaagcga aaattaaaaa aaaattatat gtaacatttg tgaatatttc cgagcgaata
      721 gtgttgcaaa acgcgcgtgt ggttctttgt gctgcaagtt aaaatacaat tcaagttggc
      781 aatacgcgca aaattgtcag ctgcgatagc taggaaaagc ctccaaaatt gagctcctaa
      841 ccgcgcccac aattgccata tcgacgccct cgccgcagca gcaacaccaa cagcagcagc
      901 agcagcagca gcagcaactc tatcagcaac atcaacagca gcagcagcaa cattacggtc
      961 caccaccgcc ctactttcaa cagctacacc agcaacacca acagcagcag caacaacagc
     1021 agcagcagca acaccagcaa cacatgaagt ttttgggtgg taacgatgat cgcaatggcc
     1081 gcggaggcgt cggcgttggc acggatgcca ttgtaggatc tcgaggtggc gtctctcagg
     1141 atgccgccga tgcagctggt gccgccgcag ccgccgccgt cggctatgtc ttccagcagc
     1201 gtccatcgcc tggtggggtt ggcgtcggcg tgggcggagt gggtggcggt gtgccagggg
     1261 tcggagccgt aggctcaacc ttgcacgagg ccgccgccgc cgagtacgcc gcccactttg
     1321 cccagaagca acagcagacc cgatgggcgt gcggcgacga cggccatggg atcgataacc
     1381 cggacaaatg gaagtacaat ccgccgatga atccggccaa tgccgctcct ggcggtccac
     1441 cgggaaatgg cagtaatggt gggcccggcg ccattggaac cattggcatg ggcagcggat
     1501 tgggtggtgg tggcggcggc ggagctggcg gcggaaataa tggcggctct ggtacgaatg
     1561 gcggtctgca tcatcaatcg atggccgctg cagctgcgaa tatggcagcc atgcaacagg
     1621 cggcggcgtt ggccaagcac aatcacatga tatcacaggc agcagccgca gttgcagccc
     1681 agcaacaaca tcagcatcca caccagcagc atccccagca gcagcagcaa cagcagcagg
     1741 cgcagaacca ggggcatcca catcacctta tgggcggtgg caatggactg ggcaacggca
     1801 atggattggg catacaacat cccggccagc aacagcagca gcagcagcaa caacagcagc
     1861 agcaacatcc cggccagtac aacgcgaatc tgcttaacca tgcggctgcc ttgggtcaca
     1921 tgtcatctta tgcccaatcg ggtggcagca tgtacgacca tcatggtgga gccatgcacc
     1981 cgggaatgaa cggcggcatg cccaagcaac agccattggg tccacccgga gccggaggac
     2041 cccaggacta tgtctacatg ggtggccaga ccactgtgcc catgggagcc gcaatgatgc
     2101 cgccacagaa tcaatatatg aacagctctg ctgttgcagc tgccaatcgg aatgcagcga
     2161 ttaccacatc cactgccaag aaattgtggg agaaatccga tggcaagggc gtatcctcga
     2221 gcactcccgg tggaccgttg catcccctgc agatccccgg catcggggat ccctcctccg
     2281 tgtggaagga tcacacctgg tccacacagg gcgagaatat attggtgccg cccccctcgc
     2341 gagcctacgc ccatggaggc gcctccgata cttcaaacag cggcaatgcg ggcatactga
     2401 gtccccgcga ttcgacttgc gccaaagtgg ttgaatatgt tttcagtggc tcgcccacca
     2461 acaaagatag ctcgctttcc ggattggaac cgcatttgcg gaatctaaag tttgacgaca
     2521 acgataagtc acgcgacgat aaggagaaag caaactctcc gtttgacaca aacggtttga
     2581 agaaagacga tcaggtcaca aactcaaatg gtgttgtcaa cggcattgac gatgacaagg
     2641 gcttcaatcg cactcctggt tcacgtcaac catcacctgc agaggagtcc cagccacgtc
     2701 cccccaatct actctttcct ccactgccct tcaatcacat gctcatggat catggccaag
     2761 gcatgggagg cggcttgggc ggagttgttg gatctggcaa cggagtcggc ggtggcagcg
     2821 gcggaggcgg ggcaggcggc gcttatgcgg cccaccagca gatggccgcc cagatgagtc
     2881 aattgcaacc gccgatgatg aacggcgttg gcggcggaat gccaatggca gcacagtcac
     2941 caatgttgaa tcaccaggca gctggaccca atcatatgga atctcccgga aatctcttgc
     3001 agcagcaaaa ttttgatgtt cagcaactgt ttcgctcgca gaatccgggc ctagcagcag
     3061 ttgccacaaa tgcagcggcc gcagcagcag ccgcagcagc tgccacatcg gcagcgagtg
     3121 ctgcggcagc ggtgggcgca ccacccgttc ccaacggatc gctgcagcag tcgcagcagc
     3181 aacagcagca gcagcaacaa cagcagcagc aacaacagat gcacatggcg gccgcgtcgc
     3241 aacaattttt ggccgcccag cagcaggcgc aaaatgcggc ctatgccgcc caacaggcca
     3301 cgtcctacgt catcaatccg ggccaggagg ctgccccgta tatgggcatg attgccgccg
     3361 cccagatgcc gtactatggc gtagcaccat ggggcatgta tccgggcaat ctgattccgc
     3421 aacagggaac gcagccgcgc cgccccctca ccccctcgca gcagggtgcc gagaatcagc
     3481 cgtatcaggt catcccggca ttcctcgatc acacgggctc cttgctgatg ggaggacctc
     3541 gcaccgggac gccgatgcgt ctggttagcc ccgcccccgt tctggtgccc ccgggcgcta
     3601 cccgtgccgg ccccccgccc ccgcaggggc cacagctgta tcagccgcag ccgcagacgg
     3661 cccaacagaa tctctactcg cagcagaatg gatccagtgt cggaggcctc gccttgaaca
     3721 cgagctcgtt gacgggtcgc cgcgactcct tcgaccgcag cacctccgcc ttcagtccct
     3781 cgaccatgga ctacaccagc agcggtgtgg cagcggccgc caatgcggtg aacagcacag
     3841 tggcccaggc agcagcagct gccgcagcag ccgccgcagc gcgtggcaag tggccgggag
     3901 cgatgtcggg agcggccagt ggagcctacg gagccctggg agcgggcaat gcttcggcca
     3961 gtcccctggg cgcaccaatc acgccgccgc catcggcgca atcctgtctc ctgggcagtc
     4021 gggcacctgg agccgagtcc cgccagcggc agcagcaaca acagcagctg gccgccgttg
     4081 gtctgccggc gactgcagca gctgctcagg cagcggtggc cgcggctgcc aacaatatgt
     4141 tcggatccaa cagctcgatc ttctcgaatc ccctggccat tccgggtacc gcagctgtgg
     4201 cagctgcagc ggcagcagca gcggccgcca actcgcgtca ggtggctgcc acggcagcgg
     4261 cagcagcggc ggtggcagca gcagccggcg gagtgggagg tgccccacag ccaggaagat
     4321 ctcgccttct cgaagatttc cgcaaccagc cgtatccaaa tcttcagcta cgcgatctcg
     4381 ctaaccacat tgtggagttc tcacaggatc agcacggctc gcggtttatc caacagaagt
     4441 tggagcgggc caccgccgcc gagaagcaaa tggtgttcag cgagatcctg gcggcagcct
     4501 atagcctgat gaccgatgtc tttggcaact atgtcatcca gaagttcttt gagttcggca
     4561 ctcccgagca gaagaacacg ctgggcatgc aggtcaaggg tcatgtgctg cagctggcgc
     4621 tgcaaatgta tggctgccga gtgattcaga aggctctgga gagcatctcg ccggagcagc
     4681 agcaggaaat cgtccacgaa ctggacggac atgtgctgaa atgcgtcaag gatcagaatg
     4741 gcaatcatgt ggtgcaaaag tgcattgagt gcgtggaccc cgtggcgctg cagttcatca
     4801 tcaatgcttt caagggtcag gtttactcgc taagcaccca tccgtatgga tgccgggtga
     4861 tccagagaat ccttgagcat tgcactgccg aacagaccac gcccattttg gacgaactgc
     4921 atgagcacac cgaacagttg attcaggacc aatatggcaa ctatgttatt cagcatgtgc
     4981 ttgaacacgg caagcaggag gataagtcga ttcttatcaa cagcgtgcgc ggcaaagttc
     5041 tggtgctatc acagcacaag ttcgcctcaa acgttgtgga gaaatgtgtt acccatgcca
     5101 ctcgcggaga acgcactggt ctcatagacg aggtctgcac cttcaacgac aacgcgttgc
     5161 acgtgatgat gaaggatcag tatgccaact atgtggtcca aaaaatgatc gatgtatcgg
     5221 agccgacgca gctcaagaag ctgatgacca agatccggcc ccacatggcc gccttgcgca
     5281 agtacaccta cggcaagcac atcaatgcca agttggagaa gtactacatg aagataacca
     5341 atcccattac ggtgggcaca ggagctggag gagtgccggc agcctcgtcg gcggccgcgg
     5401 tcagcagtgg tgccacctcg gcatcggtaa ccgcctgcac cagtggcagc agcaccacca
     5461 cgaccagcac taccaacagc ctggcctcac ccaccatttg ttcggtgcag gagaacggca
     5521 gcgccatgat tgtggagccc tcctccccgg acgcctccga gtcctcgtcc tcggtggtgt
     5581 caggcgctgt caacagcggc ttgggtccca ttggaccccc gaccaacggc aacgttgtgc
     5641 tgtaaaggaa ataacaaatt aagccaggca gtcaaaggaa acttccttct cgaatcgcag
     5701 tatagttttt agaagctgta gagcttaaca taaacaacaa gtacatataa atgtaatctt
     5761 atttattgga aaagcagcga taaatggagc tgcactcgaa gatttgcaaa gaggatagta
     5821 aaacacacat gcgccaatct agagaaacaa agcaaacaaa gaagcacact ggcaagccaa
     5881 aaagcaaaag agcttaacag ctaaaactaa aagaaatttg tatttttacg aacaaaacta
     5941 ataacgttct catgaaaaaa gatttcaaaa tatttgtaaa atgcgatcgc ataattaatt
     6001 tgtaaaaaaa aggcatgaac cgcaaagatg aaagaaaaca aaaatgcgta gttaatctcg
     6061 atcaagaaaa aataatgaat gtaatgtaaa atgtcaatga aacagatttg tctgcgtaca
     6121 ttttcgttgt aactttgtat aaattaatta ttatatagca agtctatctg taaaggatta
     6181 atgtttcgac tgtaaattaa taagaagaca actgaagagc cggcgagctg aaaaaaaaga
     6241 aagtaaaaag agcgggctgc atgaattagc ctacgattta taagttcaga cagaggaacc
     6301 atttctaata tacaaacata tatacgaggg ataacagcag aagccgcact tagtgtagaa
     6361 tgtagagtaa taatgttttt ggagccagca gctacaaaga cacaatgaaa acagagacac
     6421 acgagacacg cccacgcccc ctcacgcaca ctcggttgca tacactcaca caatgaacga
     6481 ctcttcagcc cattcacgtt gcttttgcac tatgtaaaaa ttttgtataa aaaaaaaccc
     6541 caaacaacaa accatgtaaa ccatgtaatt ttcaaatgtt tcactgtaaa atgtatacat
     6601 actttatttt gtaaattttt tttaagtcgc aagtaactca tacatattct attctaaacc
     6661 tcacgcatgt atttataatt ttatacacat tagctggtga ccaccgatcg acgatctgca
     6721 tggatgttgg tcagctggtg gccagctaaa agaacctgtt agccaagtaa gccaaaaatg
     6781 ataataattg gattttaaaa caataaccat caaaataaac caattttttt caa
//