From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Haemophilus ducreyi heat shock protein (groES and groEL) gene,
Date: 5 Nov 92 23:06:06 GMT
Distribution: bionet
Checksum: 27838 6

LOCUS       HEAGROESL    2200 bp ds-DNA             BCT       13-APR-1992
DEFINITION  Haemophilus ducreyi heat shock protein (groES and groEL) gene,
            complete cds.
ACCESSION   M91030
KEYWORDS    groEL gene; groES gene; heat-shock protein.
SOURCE      Haemophilus ducreyi (strain 35000) (library: ATCC 33922) DNA.
  ORGANISM  Haemophilus ducreyi
            Prokaryotae; Facultatively anaerobic gram-negative rods;
            Pasteurellaceae.
REFERENCE   1  (bases 1 to 2200)
  AUTHORS   Parsons,L.M., Waring,A.L. and Shayegani,M.
  TITLE     Molecular analysis of the Haemophilus ducreyi groE heat shock
            operon
  JOURNAL   Infect. Immun. 60, 4111-4118 (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     -35_signal      70..79
                     /note="heat-shock promoter"
     -10_signal      94..99
                     /note="heat-shock promoter"
     mRNA            109..2200
                     /gene="groE"
                     /evidence="EXPERIMENTAL"
                     /citation=[1]
                     /partial
     RBS             146..149
                     /gene="groE"
                     /note="putative"
                     /citation=[1]
     RBS             461..465
                     /gene="groE"
                     /note="putative"
                     /citation=[1]
     terminator      2142..2170
                     /gene="groE"
                     /note="putative"
                     /citation=[1]
     CDS             160..450
                     /note="putative"
                     /citation=[1]
                     /gene="groES"
                     /product="heat shock protein"
                     /codon_start=1
                     /translation="MSIRPLHDKVILKREEVETCSAGGIVLTGSATVKSTRGKVIAVG
                     TGRLLENGSIQPLSVKEGDMVIFNEGYGAKVEKIDGEEILILSENDILAIVE"
     CDS             473..2116
                     /note="putative"
                     /citation=[1]
                     /gene="groEL"
                     /product="heat shock protein"
                     /codon_start=1
                     /translation="MAIKDVKFGNDARVKMLKGVNILADAVKVTLGPKGRKVVLDKAY
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDVAGDGTTTATVLAQSIVSE
                     GLRAVAAGMNPMDLKRGIDKAVAAVVEELKAISKPCETSKEIEQVGTISANSDETVGK
                     LIAQAMEKVGKEGVITVEDGTGLDDALDVVEGMQFDRGYLSPYFINKPEAGTVELDNP
                     YIILVDKKISNIREILPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIAILTAGTVISEEIGMELEKATLEELGQAKRVVITKDNTTIID
                     GIGDEAQIKARVVQIRQQIEDSTSDYDKEKLQERVAKLAGGVAVIKVGAATEVAMKEK
                     KDRVDDALHATRAAVEEGIVPGGGVALVRAANKVSATLTGDNEEQNVGIKLALRAMEA
                     PLRQIVENSGEDASVVARDVKDGSGNFGYNATTEEYGDMLEMGILDPTKVTRSALQFA
                     ASIAGLMITTECMITDLPKEDKLDAQAAMGGMGGMGGMM"
BASE COUNT      742 a    315 c    520 g    623 t
ORIGIN      
        1 taagatattc ctttattttt ggtgaaaaat acaacgaaat agacggcttt ttattaaagt
       61 tttgtaaaat caccttgaaa gtaataaatt gatcccaatc tagcaaccag cacacgtgga
      121 agcgtgttgt tataattttt atgttaggag taaaacacaa tgagtattcg tccattacac
      181 gataaagtta ttttaaaacg tgaagaagta gagacctgtt cagcgggtgg tattgtttta
      241 acaggttcag cgacagttaa atcaactcgc ggtaaagtga ttgcagtagg tactggtcgt
      301 ttattagaaa atggttctat acaaccttta tctgtgaaag aaggtgatat ggtcatcttt
      361 aatgaaggtt atggagcaaa agtagaaaaa attgatggcg aagaaatttt aattctttca
      421 gagaatgaca ttcttgcaat tgtagaataa tcgaagaata agggataata aaatggcaat
      481 aaaagacgtt aaatttggta atgatgcacg cgtaaaaatg cttaaaggcg tgaatatttt
      541 agctgatgca gtaaaagtta ctttgggacc aaaaggtcgt aaagtagtat tagataaagc
      601 ctatggcgca ccaacgatca caaaagatgg cgtatctgta gcccgtgaaa ttgaattaga
      661 agataaattc gaaaatatgg gcgctcaaat ggtgaaagaa gttgcttcta aagcaaatga
      721 tgtagctggt gatggtacaa caacggcaac tgttcttgct caatctattg tcagtgaagg
      781 cttgcgcgct gttgcagcag gaatgaatcc aatggattta aaacgaggta ttgataaagc
      841 ggttgctgca gtagtcgaag aattaaaagc gatctctaaa ccatgtgaaa catctaaaga
      901 gattgaacaa gtcggtacga tctcggctaa ctctgatgaa acggttggta agcttattgc
      961 tcaagccatg gaaaaagttg gtaaagaagg ggttattacg gttgaagatg gtacaggttt
     1021 agatgatgcc ttagatgttg tggaaggtat gcagtttgat cgtggttatt tatcgccata
     1081 ctttatcaat aaaccagaag ccggtacggt tgaattagac aacccatata tcattttagt
     1141 tgataaaaaa atcagtaata tccgtgaaat tttaccggtt ttagaagcag tggcaaaagc
     1201 gggtaaacca ttattaatta ttgcagaaga tgttgaaggt gaagcgttag caactttagt
     1261 ggttaatact atgcgtggta ttgttaaagt ggctgcagtg aaagcaccgg gcttcggcga
     1321 ccgtcgtaaa gcaatgttac aagatattgc cattttaaca gcaggtaccg ttatttctga
     1381 agaaattggt atggagctag aaaaagcaac attagaagaa ttaggtcaag ctaaacgcgt
     1441 agtaatcact aaagacaaca ctactattat tgatggtatt ggtgatgaag cacaaattaa
     1501 agcacgcgta gttcaaatcc gtcagcaaat tgaagattca acttcagatt atgataaaga
     1561 aaaattacaa gaacgcgtgg ctaaattagc cggtggtgtg gcagttatta aagttggcgc
     1621 agctactgaa gttgcaatga aagagaaaaa agatcgtgtg gatgatgcac tccatgcaac
     1681 gcgtgctgca gttgaagaag gaattgtccc agggggcggt gttgcattag ttagagcagc
     1741 aaataaagta tcagctacgt taaccggcga taatgaagaa caaaatgtag gtattaaatt
     1801 agcattacgt gcaatggaag cgccattacg tcaaattgta gaaaattcag gcgaagatgc
     1861 atctgtggtt gcgcgcgatg ttaaagatgg tagcggtaac tttggctata atgcgaccac
     1921 ggaagaatat ggggatatgt tagaaatggg tattttagat ccgactaaag ttactcgttc
     1981 agcacttcaa tttgcagcgt caattgcagg attgatgatt acaacggaat gtatgattac
     2041 cgatcttcca aaagaagata aattagatgc tcaagccgct atgggtggaa tgggcggtat
     2101 gggtggaatg atgtaatcat tcttttcata atatcttttt taaagccttt gagattttct
     2161 caaaggcttt ttatttggta agcggtgaat tttacagaaa
//