From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: A.vinelandii gene for 2-oxoglutarate dehydrogenase
Date: 3 Jan 93 22:03:31 GMT
Distribution: bionet
Checksum: 45418 7

LOCUS       AVI2OGDH     3060 bp ds-DNA             BCT       21-DEC-1992
DEFINITION  A.vinelandii gene for 2-oxoglutarate dehydrogenase
ACCESSION   X52433
KEYWORDS    2-oxoglutarate dehydrogenase.
SOURCE      Azotobacter vinelandii DNA.
  ORGANISM  Azotobacter vinelandii
            Prokaryotae; Gracilicutes; Scotobacteria; Aerobic rods and cocci;
            Azotobacteraceae.
REFERENCE   1  (bases 1 to 3060)
  AUTHORS   Schulze,E., Westphal,A.H., Hanemaaijer,R. and de Kok,A.
  TITLE     The 2-oxoglutarate dehydrogenase complex from Azotobacter
            vinelandii
  JOURNAL   Eur. J. Biochem. 187, 229-234 (1990)
  STANDARD  full automatic
COMMENT     *source: strain=ATCC478;
               From EMBL    entry AV2OGDH;  dated 30-NOV-1992.
FEATURES             Location/Qualifiers
     RBS             82..88
     CDS             98..2929
                     /product="2-oxoglutarate dehydrogenase"
                     /codon_start=1
                     /translation="MQDSVMQRMWNSAHLSGGNAAYVEELYELYLHDPNAVPEEWRTY
                     FEKLPAEAGTSTDVPHAPVRDQFVLLAKNQRRAQPVATSSVSTEHEKKQVEVLRLIQA
                     YRTRGHQASQLDPLGLWQRTAPSDLSITHYGLTNADLDTPFRTGELYIGKEEATLREI
                     LQALQETYCRTIGAEFTHIVDSEQRNWFAQRLESVRGRPVYSKEAKSHLLERLSAAEG
                     LEKYLGTKYPGTKRFGLEGGESLVPVVDEIIQRSGSYGTKEVVIGMAHRGRLNLLVNA
                     LGKNPRDLFDEFEGKHLVELGSGDVKYHQGFSSNVMTSGGEVHLAMAFNPSHLEIVSP
                     VVEGSVRARQDRRVDATGEKVVPISIHGDSAFAGQGVVMETFQMSQIRGYKTGGTIHI
                     VVNNQVGFTTSNPVDTRSTEYCTDPAKMIQAPVLHVNGDDPEAVLFVTQLAVDYRMQF
                     KRDVVIDLVCYRRRGHNEADEPSGTQPLMYQKIAKQPTTRELYADALVKEGSLSQEEV
                     QAKVDEYRTALDNGQHVLKSLVKEPNTELFVDWTPYLGHAWTARHDTSFELKTLQELN
                     AKLLQIPEGFVVQRQVAKILEDRGRMGVGAMPINWGCAETLAYATLLKEGHPVRITGQ
                     DVGRGTFSHRHAALHNQKDASRYIPLQNLYEGQPKFELYDSFLSEEAVLAFEYGYATT
                     TPNALVIWEASSGDFANGAQVVIDQFISSGETKWGALCGLTMLLPHGYEGQGPEHSSA
                     RLERYLQLCAEQNIQVCVPTTPAQVYHMLRRQVIRPLRKPLVALTPKSLLRHKSAIST
                     LEDLALGSFHPVLPEVDSLDPKKVERLVLCSGKVYYDLLDKRHAEGREDIAIVRIEQL
                     YPFPEEELAEVMAPYTNLKHVVWCQEEPMNQGAWYCSQHHMRRVASAHKKELFLQYAG
                     REASAAPACGYASMHAEQQEKLLQDAFTV"
     CDS             2972..>3060
                     /product="succinyl transferase"
                     /codon_start=1
                     /translation="MAIDIKAPTFPESIADGTVATWHKKPGEPV"
BASE COUNT      630 a    996 c    905 g    529 t
ORIGIN      
        1 gatccgtagt ggctttacca aagtcgctgc tccatcgact ttggaacgac catgtgcggc
       61 tcgcgccgat ggtatcccct caagagggtg accaagcatg caagatagcg ttatgcagcg
      121 catgtggaac agtgcccacc tttccggtgg aaatgctgct tatgtggaag agctctatga
      181 gctttacctg cacgacccca acgctgtgcc cgaagagtgg cgcacctatt tcgagaagct
      241 gcccgccgaa gccggtacct ccaccgatgt tccgcacgct cccgtccgcg accagttcgt
      301 tctgctggcc aagaaccagc ggcgtgcaca gccggtcgca acctccagcg taagcaccga
      361 acacgagaag aagcaggtcg aagtcctgcg tctgatccag gcttaccgca cgcgcggcca
      421 tcaggcctcg caactggatc cgctcggtct gtggcagcgc actgcgcctt ccgacctgtc
      481 gatcacccat tacgggctga ccaacgccga tctggatacc cccttccgta ccggagagct
      541 ctacatcggt aaggaagagg cgaccctacg cgaaatcctg caggcattgc aggagacata
      601 ttgccgcact atcggcgccg aattcaccca tatcgtcgat tccgagcagc gcaactggtt
      661 cgcccagcgc ctggaaagcg tacgcggtcg tccggtgtac tccaaggagg cgaaaagcca
      721 cctgctcgag cgcctgagcg ctgccgaagg cctggaaaaa tacctgggca ccaaatatcc
      781 gggcaccaag cgcttcggtt tggaaggcgg cgagagcctg gtgccggtcg tcgacgagat
      841 catccagcgc tcaggctcct acggcaccaa ggaagtcgtc atcggcatgg ctcaccgcgg
      901 ccgtctgaac ctgctggtca acgcgctggg caagaatcca cgcgacctgt tcgacgagtt
      961 cgaaggcaag catctggtcg aactgggctc cggtgacgtg aagtaccacc agggcttctc
     1021 ctccaacgtg atgacctcgg gtggcgaagt gcacctggcc atggcgttca acccctcgca
     1081 cctggaaatc gtctccccgg tggtcgaggg ctccgtgcgt gcccgccagg accgtcgcgt
     1141 cgacgctacc ggcgagaagg tggtaccgat ctccatccac ggcgactcgg cctttgccgg
     1201 tcagggcgtg gtgatggaaa ccttccagat gtcgcagatc cgcggctaca agacgggcgg
     1261 taccattcac atcgtggtca acaaccaggt cggcttcacc accagtaacc cggtcgacac
     1321 ccgttcgacc gagtactgca ccgatccggc gaagatgatt caggcgccgg tactgcacgt
     1381 caacggcgac gatccggaag ccgtgctgtt cgtgacccaa ctggccgtcg actatcgcat
     1441 gcagttcaag cgcgacgtag tcatcgatct ggtctgctac cgccgtcgcg gtcacaacga
     1501 ggccgacgag ccgagcggca cccagccgct gatgtaccag aagatcgcca agcagcccac
     1561 cacccgcgag ctgtatgccg acgcgctggt caaggagggc agcctgagcc aggaagaagt
     1621 ccaggccaag gtcgacgaat accgtaccgc gctggataac ggtcagcacg tgctcaagag
     1681 cctggtcaag gagccgaaca ccgagctgtt cgtcgactgg accccctatc tgggccatgc
     1741 ctggaccgct cgtcacgaca ccagcttcga gctgaagacc ctgcaggaac tgaacgccaa
     1801 gctgctgcag atcccggaag gtttcgtggt ccagcgccag gttgccaaga tcctcgagga
     1861 tcgcggccgc atgggcgtcg gcgccatgcc gatcaactgg ggctgcgccg agaccctggc
     1921 ctacgccact ctgctgaagg aaggccatcc ggtacgcatc accggtcagg acgtcggccg
     1981 tggcaccttc tcgcaccgcc atgccgcgct gcacaaccag aaggatgcca gccgctacat
     2041 cccgctgcag aacctctacg agggacagcc gaagttcgag ctgtatgatt ccttcctctc
     2101 ggaagaggcc gtgctggcct tcgaatacgg ctatgccacc accacgccga acgcgctggt
     2161 gatctgggaa gccagttccg gcgacttcgc caacggtgcc caggtggtga tcgaccagtt
     2221 catcagcagc ggcgagacca agtggggagc cctgtgcggc ctgaccatgc tgctgccgca
     2281 cggctacgag ggccagggtc cggagcactc ttccgcacgt ctggagcgct acctgcaact
     2341 gtgcgccgag cagaacatcc aggtgtgcgt gccgaccacc ccggcgcagg tctaccacat
     2401 gctgcgtcgc caggtgatcc gcccgctgcg caagccgctg gtggccttga cgccgaagtc
     2461 gctgctgcgt cacaaatcgg cgatctccac cctggaagat ctggctctcg gctccttcca
     2521 tccggtcctg ccggaggtcg atagcctcga tccgaagaag gtcgagcgtc tggtcctgtg
     2581 cagcggcaag gtctactacg acctgctgga caagcgccat gccgaaggtc gcgaggacat
     2641 cgccatcgtc cgtatcgagc agctctatcc gttcccggaa gaggaactgg ccgaggtcat
     2701 ggcgccgtac accaacctca agcatgtggt ctggtgtcag gaagagccga tgaaccaggg
     2761 cgcctggtac tgcagtcagc atcacatgcg tcgcgtcgcc agcgcgcaca agaaggagct
     2821 gttcctccag tatgctggtc gcgaggcgtc ggccgctccg gcttgcggtt acgcgtcgat
     2881 gcatgccgag cagcaggaaa aactgctgca ggacgctttc actgtttaac gccttctcgc
     2941 agaagcaacg aatcaatagg aactctagat aatggctatc gatatcaaag ccccgacttt
     3001 cccggaatcg atcgcggacg gcaccgtcgc cacctggcac aaaaagcccg gcgaaccggt
//