From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human Ig germline kappa L-chain V-region pseudogene a, V-kappa-1
Date: 12 Sep 92 10:02:40 GMT
Distribution: bionet
Checksum: 08423     6

LOCUS       HUMIGKVI1    2350 bp ds-DNA             PRI       12-SEP-1992
DEFINITION  Human Ig germline kappa L-chain V-region pseudogene a, V-kappa-1
            subgroup, clone V21.
ACCESSION   K02093
KEYWORDS    constant region; germline; immunoglobulin light chain;
            kappa-immunoglobulin; kappa-immunoglobulin subgroup vk-1;
            pseudogene; variable region.
SEGMENT     1 of 5
SOURCE      Homo sapiens (library: cosmid) placenta DNA.
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 2350)
  AUTHORS   Pech,M., Jaenichen,H.-R., Pohlenz,H.-D., Neumaier,P.S.,
            Klobeck,H.-G. and Zachau,H.G.
  TITLE     Organization and evolution of a gene cluster for human
            immunoglobulin variable regions of the kappa type
  JOURNAL   J. Mol. Biol. 176, 189-204 (1984)
  STANDARD  full staff_review
COMMENT     [1] has isolated six V-region genes along an 80 kb stretch of DNA.
            Five of them were sequenced and are presented in this and following
            segments.  The six genes in order of position are a, b, c, d, e,
            and f.  All of them are of subgroup 1, except c, which is of
            subgroup 2.  Genes a and c are pseudogenes.  Two additional
            V-region genes were isolated, a' and b', and are in the same
            configuration as a and b.
FEATURES             Location/Qualifiers
     CDS             join(427..481,608..903)
                     /pseudo
                     /gene="IGK"
                     /codon_start=1
                     /partial
     sig_peptide     join(427..481,608..618)
                     /gene="IGK"
                     /codon_start=1
     intron          482..607
                     /gene="IGK"
     exon            608..903
                     /number=2
                     /gene="IGK"
     mat_peptide     619..903
                     /pseudo
                     /pseudo
                     /gene="IGK"
                     /codon_start=1
                     /partial
     iDNA            (903.905)..>2350
                     /note="V-J intervening DNA"
                     /gene="IGK"
     misc_signal     906..933
                     /note="putative recombination recognition sequence"
                     /note="putative"
BASE COUNT      685 a    497 c    510 g    657 t      1 others
ORIGIN      302 bp upstream of PstI site on chromosome 2.
        1 cgggatcata tgagagtctt ttggagacct gataatcata ccgtctaaca ttttattata
       61 tatttcctac aaacaagaat attctcctaa ataatcccca tacaccaatg aaatacatta
      121 ctccatcaac tcctgaggaa tatttcaaat tgtcaaaaaa aacctaaaaa atgtctctca
      181 taataaaata gttcccagta gaaacacatt ctctggagac aaatttgtgc taccctggtc
      241 ttacctggga cacctgggga caytgaactg gtgctgagtt actgagatga gccagccctg
      301 cagctgtgcc cagcctgccc atcctctgct catttgcata ttcccagaac acaacctcct
      361 gccctgaaga cttcttaata ggctggtcac acttcttgca ggagtcagac ccactcagga
      421 cacagcatgg acatgagggt ccccgctcag ctcctggggc ttctgctgct ctggctccca
      481 ggtaaggaag gagaacacta gcagtttact cagcccaggg ggctcagtac agcctggcta
      541 ttcagggaaa ttctcttact acatgattaa ttgtgtggac catttgtgtt tatgcttcca
      601 atctcaggtg ccagatgtgc catccagttg acccagtctc catcctccct gtctgcatct
      661 gtaggagaca gagtcaccat cacttgccgg gcaagtcagg gcattagcag tgctttagcc
      721 tgatatcagc agaaaccagg gaaagctcct aagctcctga tctatgatgc ctccagtttg
      781 gaaagtgggg tcccatcaag gttcagcggc agtggatctg ggacagattt cactctcacc
      841 atcagcagcc tgcagcctga agattttgca acttattact gtcaacagtt taataattac
      901 cctcacatag tgttacaaac ccgaacataa acccccaggg aagcagatgt gtgagactgg
      961 gccgccccag ctgcttctcc tgatgcctcc attggctgag agtgttcctc agatgcagcc
     1021 acactctgat ggtgttggta gaggaggata tgagatcacc tctgcatccc aatttctttt
     1081 tcttttctca gccccagctg cacagacatt acaatgcctc tgctgattta ataaagatag
     1141 agatcatgac acctgaagag tctagtttat ggctttggtt agaattcata taacagagaa
     1201 gaagccatta tagatattct aagcaggaat agtcttaata gatagaatta gagtctaaag
     1261 tattgaagtc taaataaaat gtacagataa atttaatgtt ttatttgcta agaaattttt
     1321 gccaaatggg gcatacagga aaactcaatg gtcttcaata tgttggaaga gcaaagagtt
     1381 ttattaaaag ggaaattatt acctattgtt ctttgagaaa ttttgttggc tgtagtaagg
     1441 gttgggagct ggcaagctca gactggtaag cagtggtggt caaactgaat cctagaatta
     1501 tattaagtta tctcagaagt tgtgggtaaa tttgctttca ggttacaata agccaaagca
     1561 gtgaagcttg cagagaattt tgttactgaa atgccaggga ttcagtatag atcctgcggc
     1621 tcaccacaca gaaagccaat cactaagaca acaagtgttg tcaaagaaca ggctttaatc
     1681 aggtgctgca gccgaggaga cgggacacca ttctcaaatg tatctccctg acaaaataaa
     1741 ttaggcgttt atatagcagg gaagaaatgt ggaaaacagg aattagagag gggtaaggaa
     1801 gataatttag tcaacaggaa gctggaggtc agttaggcaa tcataatggg tgaagggtct
     1861 gatgtctcac tgtccccatt cagtgatata taactttcag ctccttgata gtatctagag
     1921 gcctgatggt tggtttcctg aaaaaagaac tcagattaac aaatgtaact accttgagtt
     1981 ttaagactgg gggagtcagt ttctatgttt attcaaaaaa tcataaacat tagttccatg
     2041 ggataatagg gtctatttca attgcattct tcagacaata ttttgcaccc tgagtggttt
     2101 tccctcctgg tttcttggct ctgttgggta tgtcaagaat gaccgaattc tatgattaac
     2161 ttttacacta caacctttca aagccaagga tatagtagtc aggcaggttg acattagaag
     2221 caggattctc tggtactccc tcagaaaata gaatgctctc gccactgaag tatgggctat
     2281 ctaaccatgt ggtcctcatc ctgtctgaaa gcttaagggt ggggttgcag ctgctctcag
     2341 cttcctatag
//