From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: lytRABC divergon: lytR=lyt divergon expression
Date: 6 Jan 93 06:57:56 GMT
Distribution: bionet
Checksum: 29468 10

LOCUS       M250984S2    4268 bp    DNA             BCT       11-DEC-1992
DEFINITION  lytRABC divergon: lytR=lyt divergon expression
            attenuator...lytC=N-acetylmuramoyl-L-alanine amidase [Bacillus
            subtilis, 168, Genomic, 4 genes, 4268 nt, segment 2 of 2] 
ACCESSION   S47057 M87645
KEYWORDS    .
SEGMENT     2 of 2
SOURCE      Bacillus subtilis 168
  ORGANISM  Bacillus subtilis
            Unclassified.
REFERENCE   1  (bases 1 to 4268)
  AUTHORS   Lazarevic,V., Margot,P., Soldo,B. and Karamata,D.
  TITLE     Sequencing and analysis of the Bacillus subtilis lytRABC divergon:
            a regulatory unit encompassing the structural genes of the
            N-acetylmuramoyl-L-alanine amidase and its modifier.
  JOURNAL   J. Gen. Microbiol. 138 ( Pt 9), 1949-1961 (1992)
  STANDARD  full automatic
COMMENT     This entry [NCBI gibbsq 116645] was created by the journal scanning
            component of NCBI/GenBank at the National Library of Medicine.
            This sequence comes from Fig. 2.  The authors begin their numbering
            at 1013.
            Region: lytRABC divergon.  
FEATURES             Location/Qualifiers
     CDS             184..492
                     /note="For the protein sequence (NCBI gibbsq 116647):
                     Method: conceptual translation with partial peptide
                     sequencing.  This sequence comes from Fig. 2."
                     /gene="lytA"
                     /product="LytA"
                     /codon_start=1
                     /translation="MKKFIALLFFILLLSGCGVNSQKSQGEDVSPDSNIETKEGTYVG
                     LADTHTIEVTVDNEPVSLDITEESTSDLDKFNSGDKVTITYEKNDEGQLLLKDIERAN
                     "
     CDS             516..2633
                     /note="TTG start codon.  LytB;  For the protein sequence
                     (NCBI gibbsq 116648):  Method: conceptual translation with
                     partial peptide sequencing.  This sequence comes from Fig.
                     2.
                     Author-given protein sequence differs from the conceptual
                     translation for reasons explained in citation"
                     /gene="lytB"
                     /product="N-acetylmuramoyl-L-alanine amidase modifier"
                     /codon_start=1
                     /translation="MKSCKQLIVCSLAAILLLIPSVSFAADSNISVKLLNYIGNKSSI
                     SLSPTGFYKVTGDNVAVTDRFAGASRYETATLASNSQWKNPNTVILVNRDIFIDALPV
                     IPLAKKLNAPVLFTQPDTLTKTTERQIAKFNPDNILIIGGARSISKDVENKLKSYGAV
                     KRISGKNRYVLSENIAKQMGSYDKAIVVTGRVFQDALAIAPYAAAHGYPILLTEKDKL
                     PDYDLPKQVIIIGSSFSVSDSVENQIKKTSTVQRIPGSTRYELTANIIKQLKLKADKV
                     VMTNGTKYADVLIGASLASKKNSQILFVKQDSVPAAAKSITKDKATYAYDFIGSTSSI
                     SAEVENSLADEFYLADGGTYNLKINSGKLNLENIKTYGNSLRIKPENYSTSNRISLDG
                     KQYLGTVNFSIESTKYIRPVNENIPFEDYLKGVIPNEMPASWSLEALKAQTVAARTYS
                     ITKTGTTVPDTTAFQVYGGYSWNSNTNKAVEQTKGKVLKYNGSLITAAYSSSNGGYTE
                     ASNEVWSSSVPYLIAKKDTKDPQIGWTLTLSKQQLDTKSLDLTKPSSWWSSATETDSA
                     RLSGVKNWILKNKETSADSVKIASIDDLSFSGTTQGQRAKTASMKVKYFVKSSTGSYN
                     LSKITTISVPTSELRTMIGATVFKSTYVTVKKDTSKYTISGKGYGHGIGMSQYGAKAR
                     AEAGDSYSSILKFYYPGTTLTSY"
     CDS             2672..4162
                     /note="TTG start codon.  LytC;  For the protein sequence
                     (NCBI gibbsq 116651):  Method: conceptual translation with
                     partial peptide sequencing.  This sequence comes from Fig.
                     2.
                     Author-given protein sequence differs from the conceptual
                     translation for reasons explained in citation"
                     /gene="lytC"
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /codon_start=1
                     /translation="MRSYIKVLTMCFLGLILFVPTALADNSVKRVGGSNRYGTAVQIS
                     KQMYSTASTAVIVGGSSYADAISAAPLAYQKNAPLLYTNSDKLSYETKTRLKEMQTKN
                     VIIVGGTPAVSSNTANQIKSLGISIKRIAGSNRYDTAARVAKAMGATSKAVILNGFLY
                     ADAPAVIPYAAKNGYPILFTNKTSINSATTSVIKDKGISSTVVVGGTGSISNTVYNKL
                     PSPTRISGSNRYELAANIVQKLNLSTSTVYVSNGFSYPDSIAGATLAAKKKQSLILTN
                     GENLSTGARKIIGSKNMSNFMIIGNTPAVSTKVANQLKNPVVGETIFIDPGHGDQDSG
                     AIGNGLLEKEVNLDIAKRVNTKLNASGALPVLSRSNDTFYSLQERVNKAASAQADLFL
                     SIHANANDSSSPNGSETYYDTTYQAANSKRLAEQIQPKLAANLGTRDRGVKTAAFYVI
                     KYSKMPSVLVETAFITNASDASKLKQAVYKDKAAQAIHDGTVSYYR"
BASE COUNT     1506 a    732 c    841 g   1189 t
ORIGIN      
        1 cctttgcacc tcgtctgtta aattactttc attatgagtt aaatttccaa taaatacaac
       61 tctatttaat acaagaatga aatcctaaaa tagtcgtttt ttataaaaaa aagtttcatt
      121 gtttcttaat ttttctttaa aatataaaat aggttgacga taaaatataa tgaggtgaaa
      181 aaaatgaaaa aatttattgc tttactgttc tttatattgc ttctttcggg ttgcggggtt
      241 aatagtcaaa agagtcaagg tgaagatgta tcgccagaca gtaacattga aacaaaagaa
      301 ggtacttatg tagggttagc tgatactcat acaatagaag taacagtaga taatgagccg
      361 gttagtcttg atatcactga agaatcgaca agtgatcttg ataagtttaa cagtggagat
      421 aaggtcacga ttacatatga aaaaaatgat gagggtcagc ttctgttaaa agatattgaa
      481 cgtgccaact aagatatagg gaggaacctg aaattttgaa atcttgcaaa caattgatag
      541 tgtgttcact tgctgcaatc ttattattga ttccatcagt ttcttttgca gcagactcaa
      601 acatctcagt taaattgtta aattatattg gaaataaatc tagcatcagt ctatcgccaa
      661 caggatttta taaagtaact ggagataatg ttgctgttac ggatcgtttt gcaggcgctt
      721 cacgatacga aactgcgact cttgcatcca acagccagtg gaaaaatcct aatacagtga
      781 tattggtgaa ccgagatatt tttattgatg cattgcctgt tattccattg gcgaaaaaac
      841 tgaacgcacc agttttattt actcagcctg acacgttaac aaaaactact gaaagacaaa
      901 ttgctaagtt taaccctgat aatattttaa tcattggggg agcgcgaagc atttcaaaag
      961 acgtagaaaa caagctgaaa tcatatggcg ctgtgaaaag gataagcgga aaaaatagat
     1021 atgtcttatc agaaaatatt gcaaagcaaa tgggcagcta tgataaagcg atcgttgtaa
     1081 ccggaagagt atttcaagac gctttagcca ttgctccata tgcagcagca cacggatacc
     1141 ctattttgct tacagagaaa gacaagcttc cggattatga tttgccaaaa caagtcatca
     1201 ttataggcag ttcatttagc gtcagtgata gtgtagaaaa ccaaattaaa aaaacttcaa
     1261 ctgtgcaaag gattcctgga tcaaccagat atgaacttac ggcaaatata attaagcaac
     1321 taaagttgaa ggctgataaa gtcgtaatga cgaacgggac aaaatatgca gacgtattga
     1381 taggcgcttc gcttgcctcg aagaaaaaca gccaaatttt atttgttaaa caagatagcg
     1441 ttccggctgc ggctaaaagt ataacgaaag ataaagccac atatgcatac gactttattg
     1501 gaagtaccag ttcaatttct gctgaagttg agaattcact tgctgatgaa ttttatttag
     1561 ctgacggtgg cacctataat ctaaaaatca acagcggaaa attaaacctt gaaaacatca
     1621 aaacttacgg aaactcccta agaattaaac cagaaaacta ttctacttca aacagaatta
     1681 gtcttgatgg aaagcagtat cttggcacgg ttaatttctc tattgagtct actaaatata
     1741 tccgaccggt aaatgagaat attccatttg aggattactt aaaaggagta attccgaatg
     1801 aaatgcctgc tagctggtca cttgaggctc ttaaagcaca gactgtcgct gcaagaacct
     1861 actcaataac aaaaacagga actactgtac ctgatacaac cgcttttcaa gtttatggtg
     1921 gctacagttg gaattcgaat accaataaag cggtagagca aacgaagggg aaagttctga
     1981 aatataatgg atctcttata actgcagctt attcttccag taatggtggt tacacagaag
     2041 ctagtaatga agtgtggtcg tcaagtgtac cttacttgat tgcaaaaaaa gatacaaagg
     2101 atcctcaaat aggctggacg cttactttgt caaaacaaca gctagatacg aaatctttgg
     2161 acttaacgaa gccttcttct tggtggtctt cagcgactga aactgattct gcaagacttt
     2221 ccggagttaa gaactggatc ttgaaaaata aagaaacaag cgctgattct gtcaaaattg
     2281 caagtattga cgatttaagc tttagtggaa cgacacaagg gcagcgtgca aaaacagcat
     2341 cgatgaaagt gaagtatttt gttaaaagca gtactggctc ctataacttg agcaaaatta
     2401 caaccattag tgtaccaaca agtgagttaa gaacaatgat tggagcgact gtatttaaaa
     2461 gtacttatgt gactgtaaaa aaagatactt caaagtacac aatcagcggt aaaggatatg
     2521 gtcacgggat cggaatgagt caatatggag caaaagcaag agcagaagct ggagattcat
     2581 atagttcaat tttgaaattt tactatccag gtacaactct gacaagttat tgattatttt
     2641 aggatataac gccatttaag gaggaaatca attgcgttct tatataaaag tcctaacaat
     2701 gtgttttctg gggctcatac tttttgtgcc aacagctttg gccgataact cagtgaaaag
     2761 agttggggga agcaatagat acggcactgc tgtacaaata tcaaagcaaa tgtattcaac
     2821 agcaagtaca gctgtaattg ttggtgggag ttcctatgca gatgctattt cagcagcacc
     2881 tcttgcttac cagaagaatg cgccattgct ttacactaat tctgataagc tttcatatga
     2941 aacgaaaaca agattgaaag agatgcagac taaaaatgta attattgtag gcggaacacc
     3001 tgctgtttct tctaacactg ctaaccagat taaaagcttg gggataagta ttaaacgaat
     3061 tgcaggaagc aaccgttatg atacggctgc acgggtggca aaagcgatgg gtgcgacttc
     3121 aaaagctgtt attttgaacg gcttcttata tgcagacgct ccggccgtca tcccttatgc
     3181 agcgaaaaac gggtatccaa ttctttttac aaataaaaca tctataaata gtgcgactac
     3241 gtctgtgata aaagataagg gaatttcgag taccgttgtt gtaggaggca ctggaagtat
     3301 cagcaatacg gtatacaaca agttaccttc tcctacaaga attagcggtt caaacagata
     3361 tgagcttgct gcaaatatcg tacaaaaact taatttatca acaagcaccg tatatgtaag
     3421 caatggattc agctaccctg actctattgc aggagctaca ctggcagcta agaagaagca
     3481 atctcttatt cttacaaatg gtgaaaattt atctacagga gcccgtaaaa ttattggaag
     3541 taaaaacatg tcaaacttta tgattatcgg aaacactcct gccgtaagca caaaggttgc
     3601 taatcagcta aagaatccag ttgtaggtga aacaatcttt attgatccgg gtcacggtga
     3661 tcaagattca ggagcaatcg gcaatggact ccttgagaaa gaagtcaacc ttgatatagc
     3721 gaaaagagtc aatacaaagc taaatgcttc aggtgctctt ccagtactgt caagatctaa
     3781 tgatactttt tattctttac aggagagagt aaataaagca gcttctgcac aagcagattt
     3841 atttctcagt atacatgcaa atgctaatga tagctcatca ccaaatggaa gtgagacgta
     3901 ctacgataca acatatcaag ctgcaaatag caagagactg gctgaacaaa ttcaaccaaa
     3961 gttagcggct aatcttggaa cgagagaccg gggagtaaaa acagctgctt tctatgttat
     4021 taaatattct aaaatgccga gtgttttagt tgaaactgcc tttatcacta atgcatcaga
     4081 tgcaagtaaa ttgaagcaag cggtttataa agataaagct gcacaagcta ttcatgacgg
     4141 cacagtatct tattacagat aatcgaaaga gacaaatcta atcacagatt tgtctctttt
     4201 ttatatgaaa tgattaaaac aaataggact tgtatataaa atagtaaaag agtattaaca
     4261 acatttat
//