From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: D.pseudoobscura Adh gene for alcohol dehydrogenase
Date: 28 Dec 92 21:26:22 GMT
Distribution: bionet
Checksum: 02832 8

LOCUS       DROADHGN08   3472 bp ds-DNA             INV       01-DEC-1992
DEFINITION  D.pseudoobscura Adh gene for alcohol dehydrogenase
ACCESSION   X62188
KEYWORDS    ADH gene; alcohol dehydrogenase.
SOURCE      Drosophila pseudoobscura DNA.
  ORGANISM  Drosophila pseudoobscura
            Eukaryota; Animalia; Metazoa; Arthropoda; Uniramia; Insecta;
            Pterygota; Neoptera; Holometabola; Diptera; Brachycera;
            Cyclorrhapha; Schizophora; Drosophiloidea; Drosophilidae.
REFERENCE   1  (bases 1 to 3472)
  AUTHORS   Schaeffer,S.W. and Miller,E.L.
  TITLE     Estimates of gene flow in Drosophila pseudoobscura determined from
            nucleotide sequence analysis of the alcohol dehydrogenase region
  JOURNAL   Genetics 132, 471-480 (1992)
  STANDARD  full automatic
COMMENT     *source: strain=BR5F; *source: isolate=Bryce Canyon, Utah; *source:
            chromosome=IV section 88;
               From EMBL    entry DPADHGN08;  dated 16-OCT-1992.
FEATURES             Location/Qualifiers
     mRNA            join(40..119,915..1020,1084..1488,1549..1964)
                     /note="adult mRNA (Adh)"
     mRNA            join(882..1020,1084..1488,1549..1964)
                     /note="larval mRNA (Adh)"
     promoter        8..14
                     /note="adult promoter (Adh)"
     prim_transcript 40..1964
                     /note="adult primary transcript (Adh)"
     exon            40..119
                     /note="adult exon 1a"
     intron          120..914
                     /note="adult intron"
     promoter        846..853
                     /note="larval promoter (Adh)"
     prim_transcript 882..1964
                     /note="larval primary transcript (Adh)"
     exon            882..1020
                     /note="larval exon 1"
     exon            915..1020
                     /note="adult exon 1b"
     intron          1021..1083
                     /number=1
     exon            1084..1488
                     /number=2
     intron          1489..1548
                     /number=2
     exon            1549..1964
                     /number=3
     polyA_signal    1941..1946
     polyA_site      1964
     CDS             join(928..1020,1084..1488,1549..1815)
                     /EC_number="1.1.1.1"
                     /gene="Adh"
                     /product="alcoholdehydrogenase"
                     /codon_start=1
                     /translation="MSLTNKNVVFVAGLGGIGLDTSRELVKRNLKNLVILDRIDNPAA
                     IAELKAINPKVTITFYPYDVTVPVAETTKLLKTIFAQVKTIDVLINGAGILDDHQIER
                     TIAVNYTGLVNTTTAILDFWDKRKGGPGGIICNIGSVTGFNAIYQVPVYSGSKAAVVN
                     FTSSLAKLAPITGVTAYTVNPGITKTTLVHKFNSWLDVEPRVAEKLLEHPTQTSQQCA
                     ENFVKAIELNKNGAIWKLDLGTLEPITWTQHWDSGI"
BASE COUNT      970 a    792 c    792 g    918 t
ORIGIN      
        1 cgtagtgtat ttaaggcact gcacatgtcg aggaccacga ttattgtcta cagtgcagtc
       61 agtagttgtc aggtgcagca ggcggaccag catttcttta aatctactca atcaatcaag
      121 taagtaactg taatccaagt acagtcggcc catcccactc ggttctgccg taactcaaga
      181 aatttcttga tagcgcagtt ccagttgtaa aacgtcgtct aaaaaaacat ctcgttggag
      241 cttgggttga aaaacgttcg agttgggcgt agaaaagttc aaatataaaa tatcaaataa
      301 gagaaaatat tctcccaaag cagctgtcca ttttttgtta aagtgataga tcaaaaattc
      361 gtatgacgta tttcccaagt gctctttatg caaataattt cctcaattct aattctacaa
      421 gaagcacttt tggctcttgt ccagaaaaaa acaatttgtt tacacttttc ggttgatagc
      481 ataacgtacc gacggctaag taacataaca gtcccctgtt aagtaatgcc ctcactctct
      541 gttagatggc cgcgcgtgtc gactccgata gaccagcaga cacgcagtag ttttcctcaa
      601 gaacctgaac tctaaacata gacataattt acgatacctt gagactcaaa taaaaataca
      661 agtatgagtg gtattctatt cctgattaga ctcgcacaca cacacgtacg agtattaaca
      721 taaatttgcc ggcaaataac caaaacaaaa cgaacgcaaa cgatccgaac acgtaatgcg
      781 agagataaga aacagaaaag ctcgacgtga aagaagcttc tggcggaagc tcttgccgaa
      841 ctccctataa atacgagaca gaaaaccaga ataaatataa taacaaactg ttgaattgtc
      901 ccatcgattc ccagatcaaa agacagaatg tctctcacca acaagaacgt cgttttcgtg
      961 gccggtctgg gcggcattgg cctggacacc agccgggagt tggtcaagcg taatctgaag
     1021 gtaagagaga acgaattcca tggaatctat ggaatcctaa atttaaaaat ttatattttt
     1081 tagaacctgg tcatcctgga tcgcattgac aatccggctg ccattgccga actgaaggca
     1141 atcaatccca aggtgaccat caccttctat ccctacgatg tgactgtgcc cgtcgctgag
     1201 accaccaagc tcctgaagac catctttgcc caggtgaaga caatcgatgt cctgatcaac
     1261 ggtgctggca tcctggacga tcatcagatc gagcgcacca ttgccgttaa ctacacgggc
     1321 ctggtcaaca ccaccacagc cattctggac ttctgggaca agcgcaaggg cggcccaggc
     1381 ggcatcattt gcaacattgg ctccgtcacc ggtttcaatg ccatctacca ggtgcccgtt
     1441 tactccggct ccaaggcggc ggtggttaac tttacctcct ccctggcggt aagtaatcca
     1501 acgtttttcc tactccccaa aactaaatat ttctttgaaa tcccatagaa actggctccc
     1561 attactggtg tcactgctta cactgtcaat cctggcatca ctaagaccac tctggtccac
     1621 aaattcaact cgtggctgga tgtggagccc cgtgtggcag agaagctgct cgagcatccc
     1681 acccagacct ctcagcagtg cgccgagaac tttgtgaagg ccattgagct gaacaagaac
     1741 ggtgccatct ggaagttgga tctgggcacc ttggagccca tcacatggac ccagcactgg
     1801 gactcgggca tctaaaccat cccagagact ctatgggaca tggcgtttta gcttttagtt
     1861 tctttttttc cactcaattg ttacgtatat acatacacat atggcaataa ggctgatttg
     1921 aaccccgttt tgaatatgat aatacaaaat tatatttgag aaatttcaac aaaatcgata
     1981 agagctatag ctctctttta attaaaaaaa acttgataaa aagagcaatg gtcagtggtg
     2041 gtcagtggtc gttgtggcct ctatcgattt ccacacaaaa actttactgt ttagtaatag
     2101 aatagaactt gagaaaggca gccaaaagaa tgtacgatct gacgggtaag catgtctgct
     2161 atgtggctga ctgcggtggc attgcactgg agaccagcaa ggttctcatg accaagaata
     2221 tagcggtgag tggtagagag gtggtgtaga gagtggagag tggttctggt tcttaaacaa
     2281 gtctctagtc tttagtctct agtctcttgg ctgctgctgg tagctgtcga aactaattaa
     2341 gttaatgact ttttcatgtt ttgtttttgt ccgctgactt ttgctgttca aagctacaat
     2401 taagtttatg ttttgagttt gatctacttt tgcttaaggg aagtactttc gattacggat
     2461 tgctggcata taaaaataga taccaaggaa tgtgcatcat attgattcta tcttgattct
     2521 ttgcccagaa actggccatc cttcagagcg tggaaaaccc cccggccatc gctcagttac
     2581 aatcgattaa gcacagcaca cagatcttct tctggacctt cgatgtgacc atggcccgcg
     2641 aggagatgaa gaagtacttc gatgaggtca tggtccagat ggactacatc gatgtgctga
     2701 tcaatggggc caccctgtgc gatgagcgga acattgatgc caccatcaat acgaatctga
     2761 ccggcatgat gaacaccgtg gccactgtgc tgccctatat ggaccgtaag atgggcggtt
     2821 cgggtggact gattgtgaat gtcacctccg tcataggact ggatccatcg ccagtctttt
     2881 gtgcgtacag tgcctcaaag tttggtgtga ttgggttcac ccgaagtcta gcggtgagtg
     2941 ggcgagatct taacttgaaa atgctctcct aatgtcttcc attcttcggc aggatcccct
     3001 gtattacacc caaaatggtg tggctgtcat ggccgtctgc tgtggtccca ccaaagtgtt
     3061 tgtcgatcgg gaactgactg ccttcctgga atacggccag tcctttgccg atcgcctgcg
     3121 tcgtgccccc tgccaatcga cggccgtctg tggcaagaat atagtaaatg ccatcgagag
     3181 atcggagaac ggacagatct ggattgccga caagggcggc ctggagtcgg tggccctcca
     3241 ctggtattgg catatggccg atcagtttgt gaactacatg cagagcactg acgatgagga
     3301 tcaggagttt tttttgggcc agcgatgagg catgccttga tccacggaat aaaacatcaa
     3361 atgtgtcaca aaaagtaggc aacttgtttg tgggtctgga aatagatttg aaatattttg
     3421 aggtcatatg aataatgaca taaaagaggg tgcctgaaag tatgccaagt tt
//