From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human paraonfluenza virus type 3 (PIV-3), phosphoprotein (P) mRNA,
Date: 21 Jul 92 10:07:51 GMT
Distribution: bionet
Checksum: 64916     6

LOCUS       PIF3P        2014 bp ss-mRNA            VRL       21-JUL-1992
DEFINITION  Human paraonfluenza virus type 3 (PIV-3), phosphoprotein (P) mRNA,
            complete cds.
ACCESSION   D10029 D00048
KEYWORDS    C; P; nonstructural protein C; phosphoprotein.
SOURCE      Human parainfluenza virus type 3 (HA-1 strain 47885), cDNA to mRNA,
            clones pB26, pA14.
  ORGANISM  Human parainfluenza virus type 3
            Viridae; ss-RNA enveloped viruses; Negative strand RNA viruses;
            Paramyxoviridae.
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Luk,D., Sanchez,A. and Banerjee,A.K.
  TITLE     Messenger RNA encoding the phosphoprotein (P) gene of human
            parainfuluenza virus 3 is bicistronic
  JOURNAL   Virology 153, 318-325 (1986)
  STANDARD  full staff_review
COMMENT     The complete nucleotide sequence of the phosphoprotein (P) mRNA of
            human parainfluenza virus 3 (PIV-3) was derived from two cDNA
            clones spanning almost the entire P gene. The mRNA, excluding the
            poly(A) tail, is 2014 nucleotides long and is bicistronic. The
            first open reading frame (ORF) codes for the phosphoprotein (P) of
            mol wt 68860. Seven nucleotides downstream from the first AUG
            codon, in a +1 reading frame, there is an additional ORF which can
            code for a polypeptide of mol wt 23266. The latter protein appears
            to be similar to the C proteins found in cells infected with
            several paramyxoviruses.
FEATURES             Location/Qualifiers
     mRNA            1..2014
     CDS             80..1927
                     /product="phosphoprotein P"
                     /codon_start=1
                     /translation="MESDAKNYQIMDSWEEEPRDKSTNISSALNIIEFILSTDPQEDL
                     SENDTINTRTQQLSATICQPEIKPTETSEKVSGSTDKNRQSGSSHECTTEAKHRNIDQ
                     ETVQGGSGRRSSSDSRAETVVSGGISGSITDSKNGTQNTENIDLNEIRKMDKDSIERK
                     MRQSADVPSEISGSDVIFTTEQSRNSDHGRSLEPISTPDTRSMSVVTAATPDDEEEIL
                     MKNSRMKKSSSTHQEDDKRIKKGGGGKGKDWFKKSRDTDNQTSTSDHKPTSKGQKKIS
                     KTTTTNTDTKGQTETQTESSETQSPSWNPIIDNNTDRTEQTSTTPPTTTPKSTRTKES
                     IRTNSESKPKTQKTIGKERKDTEESNRFTERAITLLQNLGVIQSTSKLDLYQDKRVVC
                     VANVLNNVDTASKIDFLAGLVIGVSMDNDTKLIQIQNEMLNLKADLKRMDESHRRLIE
                     NQREQLSLITSLISNLKIMTERGGKKNQNESNERVSMIKTKLKEEKIKKTRFDPLMEA
                     QGIDKNIPDLYRHAGNTLENDVQVKSEILSSYNESNATRLIPRKVSSTMRSLVAVINN
                     SNLPQSTKQSYINELKHCKSDEEVSELMDMFNEDVNNAKDQIKKTTPNK"
     CDS             90..689
                     /product="nonstructural protein C"
                     /codon_start=1
                     /translation="MLKTIKSWILGKRNQEINQLISPRPSTSLNSYSAPTPKKTYRKT
                     TQSTQEPSNSAPPSVNQKSNQQKQVKKLVDQLTKIDSLGHHTNVQQKQNIEILIRKLY
                     REDLGEEAAQIVELRLWSLEESLEASQILKMEPKTRRILISMKLERWIRTLLRGKCDN
                     LQMFQARYQEVMSYLQQNKVETVIMEEAWNLSVHLIQDQ"
BASE COUNT      882 a    382 c    372 g    378 t
ORIGIN
        1 aggattaaag aatcctatca taccagaaca tagagtggta aatttagagt ctgcttgcaa
       61 ctcaatcaat agagagttga tggaaagcga tgctaaaaac tatcaaatca tggattcttg
      121 ggaagaggaa ccaagagata aatcaactaa tatctcctcg gccctcaaca tcattgaatt
      181 catactcagc accgaccccc aagaagacct atcggaaaac gacacaatca acacaagaac
      241 ccagcaactc agcgccacca tctgtcaacc agaaatcaaa ccaacagaaa caagtgaaaa
      301 agttagtgga tcaactgaca aaaatagaca gtctgggtca tcacacgaat gtacaacaga
      361 agcaaaacat agaaatattg atcaggaaac tgtacaggga ggatctggga gaagaagcag
      421 ctcagatagt agagctgaga ctgtggtctc tggaggaatc tctggaagca tcacagattc
      481 taaaaatgga acccaaaaca cggagaatat tgatctcaat gaaattagaa agatggataa
      541 ggactctatt gagaggaaaa tgcgacaatc tgcagatgtt ccaagcgaga tatcaggaag
      601 tgatgtcata tttacaacag aacaaagtag aaacagtgat catggaagaa gcttggaacc
      661 tatcagtaca cctgatacaa gatcaatgag tgttgttact gctgcgacac cagatgatga
      721 agaagaaata ctaatgaaaa atagtaggat gaagaaaagt tcttcaacac accaagaaga
      781 tgacaaaaga attaaaaaag gggggggggg aaaagggaaa gactggttta agaaatcaag
      841 agatactgac aaccagacat caacatcaga tcacaaaccc acatcaaaag ggcaaaagaa
      901 aatctcaaaa acaacaacca ccaacaccga cacaaagggg caaacagaaa cacagacaga
      961 atcatcagaa acacaatccc catcatggaa tcccattatc gacaacaaca ctgaccgaac
     1021 cgaacagaca agcacaaccc ccccaacaac aactcccaaa tcaactcgta caaaagaatc
     1081 aatccgaaca aactctgaat ccaaacccaa gacacaaaag acaattggaa aggaaaggaa
     1141 ggatacagaa gagagcaatc gatttacaga gagggcaatt actctattgc agaatcttgg
     1201 tgtaattcaa tctacatcaa aactagattt atatcaagac aaacgagttg tatgtgtagc
     1261 aaatgtacta aacaatgtag atactgcatc aaagatagac ttcctagcag gattagtcat
     1321 aggggtttca atggacaatg acacaaaatt aatacagata caaaatgaaa tgttaaacct
     1381 caaagcagat ctaaagagaa tggacgaatc acatagaaga ttgatagaaa atcaaagaga
     1441 acaactgtca ttgatcacat cgttaatttc aaatcttaaa attatgactg agagaggagg
     1501 aaagaaaaac caaaatgaat ccaatgagag agtatctatg atcaagacaa aattgaaaga
     1561 agaaaagatc aagaaaacca ggtttgaccc acttatggag gcacaaggta ttgacaagaa
     1621 tatacctgat ctatatcgac atgcaggaaa tacgttagag aacgacgtac aagttaaatc
     1681 agagatatta agttcataca acgagtcaaa tgcaacaaga ctaataccca gaaaagtgag
     1741 cagtacaatg agatcactag ttgcagtcat caacaacagc aatctcccac aaagcacaaa
     1801 acaatcatat ataaacgaac tcaaacattg caaaagtgat gaagaagtat ctgaattgat
     1861 ggacatgttc aatgaagatg ttaacaatgc taaagatcaa ataaaaaaaa caacaccgaa
     1921 taaatagaca agaaacaaca gtagatcaaa acctatcaac acacacaaaa tcaagcagag
     1981 tgaaacaata gacatcaatc aatatacaaa taag
//