From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: squalene synthase [rats, hepatoma cell line H35, mRNA, 1708 nt]
Date: 27 Jan 93 05:53:06 GMT
Distribution: bionet
Checksum: 19577 5

LOCUS       S116694      1708 bp    mRNA            ROD       14-JAN-1993
DEFINITION  squalene synthase [rats, hepatoma cell line H35, mRNA, 1708 nt]
ACCESSION   S47419 M95591
KEYWORDS    .
SOURCE      rats hepatoma cell line H35
  ORGANISM  Unclassified.
            Unclassified.
REFERENCE   1  (bases 1 to 1708)
  AUTHORS   McKenzie,T.L., Jiang,G., Straubhaar,J.R., Conrad,D.G. and
            Shechter,I.
  TITLE     Molecular cloning, expression, and characterization of the cDNA for
            the rat hepatic squalene synthase.
  JOURNAL   J. Biol. Chem. 267, 21368-21374 (1992)
  STANDARD  full automatic
COMMENT     This entry [NCBI gibbsq 116694] was created by the journal scanning
            component of NCBI/GenBank at the National Library of Medicine.
            This sequence comes from Fig. 1.  The authors begin their numbering
            at -94.  Zero is not present in the numbering.
FEATURES             Location/Qualifiers
     CDS             95..1345
                     /note="RSS;  For the protein sequence (NCBI gibbsq
                     116696):  Method: conceptual translation with partial
                     peptide sequencing.  This sequence comes from Fig. 1."
                     /product="squalene synthase"
                     /codon_start=1
                     /translation="MEFVKCLGHPEEFYNLLRFRMGGRRNFIPKMDRNSLSNSLKTCY
                     KYLDQTSRSFAAVIQALDGDIRHAVCVFYLILRAMDTVEDDMAISVEKKIPLLRNFHT
                     FLYEPEWRFTESKEKHRVVLEDFPTISLEFRNLAEKYQTVIADICHRMGCGMAEFLNK
                     DVTSKQDWDKYCHYVAGLVGIGLSRLFSASEFEDPIVGEDTECANSMGLFLQKTNIIR
                     DYLEDQQEGRQFWPQEVWGKYVKKLEDFVKPENVDVAVKCLNELITNALQHIPDVITY
                     LSRLRNQSVFNFCAIPQVMAIATLAACYNNHQVFKGVVKIRKGQAVTLMMDATNMPAV
                     KAIIYQYIEEIYHRVPNSDPSASKAKQLISNIRTQSLPNCQLISRSHYSPIYLSFIML
                     LAALSWQYLSTLSQVTEDYVQREH"
BASE COUNT      465 a    426 c    419 g    398 t
ORIGIN      
        1 ggccccgctc ctcgccctgc ctaccgccgg catctaaaca caggtgggag tgggagatcc
       61 cgacaggtga gccccgcgcc ccgcagccac aaggatggag ttcgtgaagt gtctaggcca
      121 cccggaggag ttctacaacc tgctgcgatt ccgcatggga ggccggcgga atttcatacc
      181 caagatggac cggaactcgc tcagcaacag cttgaagact tgctataagt atcttgatca
      241 gaccagtcgc agcttcgccg cggttatcca ggcgctggat ggggacatac gtcatgcggt
      301 gtgtgtgttt tacctgatcc tccgagccat ggacacagtg gaggatgaca tggccatcag
      361 tgtggagaag aagatcccac tgctgcgaaa ctttcacact ttcctctatg agccggagtg
      421 gcggttcacc gagagcaagg agaagcaccg agtagtgctg gaggacttcc ccacgatctc
      481 cctggagttt agaaatttgg ctgagaaata tcaaacagtg atcgctgaca tctgtcacag
      541 gatgggatgt gggatggcag aatttctaaa caaggatgta acctccaaac aggactggga
      601 caagtactgt cactatgttg ctggactggt gggaatcggc ctttctcgcc tattctctgc
      661 ctcagagttt gaagatccca tagttggtga agacacagag tgtgccaatt ctatgggtct
      721 gtttctgcag aaaacaaata tcattcgtga ttatctggaa gaccaacaag aaggaagaca
      781 gttttggcct caagaggtat ggggcaaata tgttaagaag ctggaagact ttgttaagcc
      841 agagaacgta gatgtggccg tgaagtgctt gaatgaactc ataaccaacg ccctacaaca
      901 catccctgac gtcatcacct acctgtcaag gctccggaac caaagtgtgt ttaacttctg
      961 tgccattcca caggtaatgg ccattgctac gctggctgcc tgttacaata accatcaggt
     1021 attcaaggga gtagtgaaga ttcggaaggg gcaagcagtt accctcatga tggatgccac
     1081 caacatgcca gctgtcaaag ctatcatata ccagtacata gaagagattt atcaccgggt
     1141 ccccaactca gacccgtcag ctagcaaggc caagcagctc atctccaaca tcaggacgca
     1201 gagccttccc aattgccagc tcatctcccg aagccactac tcccccattt acctgtcctt
     1261 catcatgctc ttggctgccc tgagctggca gtacttgagc actctgtccc aggtcacaga
     1321 agactatgtc cagagagaac actgactttg tttagctgga agcggaagtc cacgtgaagt
     1381 gggtttttct tcttccccca gctggatttt gacttccctt ggtttttcct tctactctaa
     1441 tctttcggag aactgagtgt gggaccttta ggaactctga agaggaaagg acgccttgcc
     1501 ctcagcagcc tggtgcttcc tggatgtggt ccctgcctct tgtagccact ggcatcatgt
     1561 tgaccgaagc actggaaagg ccacatgtga tcctagtgaa cctggctaga atgctgattg
     1621 aatctattta atttgaaaca gcctttgaat acctatcaca gtaaaaaaaa aaaaaaaaaa
     1681 aaaaaaaaaa aaaaaaaaaa aaaaaaaa
//