From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Proteus vulgaris tryptophanase and tryptophan-specific permease
Date: 22 Aug 92 10:01:23 GMT
Distribution: bionet
Checksum: 42463     7

LOCUS       PRMTRY       3148 bp ds-DNA             BCT       22-AUG-1992
DEFINITION  Proteus vulgaris tryptophanase and tryptophan-specific permease
            genes, complete cds.
ACCESSION   M93277
KEYWORDS    tnaC gene; tryptophan-specific permease; tryptophanase.
SOURCE      Proteus vulgaris DNA.
  ORGANISM  Proteus vulgaris
            Prokaryotae; Gram-negative facultatively anaerobic rods;
            Enterobacteriaceae.
REFERENCE   1  (bases 1 to 3148)
  AUTHORS   Kamath,A.V. and Yanofsky,C.
  TITLE     Characterization of the Tryptophanase Operon of Proteus vulgaris:
            Cloning, Nucleotide sequence, Amino Acid Homology, In vitro
            Synthesis of the Leader Peptide and Regulatory Analysis
  JOURNAL   Unpublished (1992)
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     CDS             139..243
                     /gene="tnaC"
                     /codon_start=1
                     /translation="MFSSFNVLIILRGFVRLKKWFNIDSELAFFFPKK"
     CDS             359..1762
                     /product="tryptophanase"
                     /codon_start=1
                     /translation="MAKRIVEPFRIKMVEKIRVPSREEREAALKEAGYNPFLLPSSAV
                     YIDLLTDSGTNAMSDHQWAAMITGDEAYAGSRNYYDLKDKAKELFNYDYIIPAHQGRG
                     AENILFPVLLKYKQKEGKAKNPVFISNFHFDTTAAHVELNGCKAINIVTEKAFDSETY
                     DDWKGDFDIKKLKENIAQHGADNIVAIVSTVTCNSAGGQPVSMSNLKEVYEIAKQHGI
                     FVVMDSARFCENAYFIKARDPKYKNATIKEVIFDMYKYADALTMSAKKDPLLNIGGLV
                     AIRDNEEIFTLARQRCVPMEGFVTYGGLAGRDMAAMVQGLEEGTEEEYLHYRIGQVKY
                     LGDRLREAGIPIQYPTGGHAVFVDCKKLVPQIPGDQFPAQAVINALYLESGVRAVEIG
                     SFLLGRDPATGEQKHADMEFMRLTIARRVYTNDHMDYIADALIGLKEKFATLKGLEFE
                     YEPPVLRHFTARLKPIE"
     CDS             1842..3095
                     /product="tryptophan-specific permease"
                     /codon_start=1
                     /translation="MENTSVNKEPSIIVGGFVLGGAMIGAGMFSLPTIMSGAWFINSL
                     FILFIVCFFMFHSGIYILECISKYGAGTNYFDISKELLPKWACYIANASLIFVLYILI
                     YAYISAAGSIIYEASLLYGINFNLRAIFFIFTIALGATIWWGGACASRLTSIFLFIKI
                     VLFILAFSGLFFKAKGDLLFSATFAGKSQLYLYPFIFIIIPYAITSFGYHGNVCSLYK
                     LYNQNERKVVKSCIIGCLLALVIYLLWMIGTMGNLPREQFITIIQKGGNLDAFIDSLY
                     TVLNSKYIEGFLLWFSISAVFCSFLGVAIGLFDYILASLKFKDNKTGRLKSGVLCFTP
                     PLLLCLFFPNGFLIAIAYAGTAACVWAIICPAVMALKARQKFPNSGFKVWGGKKLIYA
                     VIAFGVVGIICQSWRNLIYCLFIVK"
BASE COUNT      970 a    457 c    597 g   1124 t
ORIGIN
        1 gatcacgtta atttataata aacactaaat agtgtgatta ttatccaatt tatactattg
       61 ttatattact gaacgaagct aactgttata taaactagtg tgtaaattgt actatttcct
      121 gactagaagg gtgaaagtat gttctcatca tttaatgtat tgataatatt aagaggtttc
      181 gtcagattga agaaatggtt taatattgac tctgaactcg ccttcttctt tcctaaaaaa
      241 taatcccttt tctatacata tctaaattaa tttaataatt cttttccgtt tatttaaaac
      301 ggagagacta ttttatttaa tctttattta ttatttaatt tatattaaag gtattatcat
      361 ggctaaaaga atcgtagaac cgttccgtat taaaatggta gaaaaaatcc gagttccttc
      421 aagagaagaa cgtgaagcag cattaaaaga agcaggatat aacccatttt tactaccaag
      481 cagcgcggta tatattgatt tattgactga ctctggaact aacgcaatga gtgatcatca
      541 atgggcagcg atgattacag gtgatgaagc ctatgcgggt tcaagaaact attatgattt
      601 aaaagataaa gcaaaagaac tttttaatta tgattatatt attccagctc accaaggtcg
      661 tggcgctgag aatattcttt ttcctgtgct attaaaatat aaacaaaaag aaggaaaagc
      721 gaaaaaccca gtttttattt ctaacttcca ctttgataca accgctgccc acgtagaatt
      781 aaatggttgt aaagcaatta atattgttac tgaaaaagcc ttcgattcag aaacctatga
      841 tgattggaaa ggcgattttg atattaaaaa attaaaagag aatattgcac aacacggtgc
      901 tgataatatt gttgctattg tatcaaccgt gacttgtaat agtgccggcg gccagcctgt
      961 ttctatgagt aatttaaaag aagtttatga aatagcgaaa caacacggta tttttgttgt
     1021 gatggattca gctcgttttt gtgagaatgc ttattttatt aaagcgcgtg atcctaaata
     1081 taaaaatgcc actattaaag aagttatttt tgatatgtat aaatatgctg atgcattaac
     1141 aatgtcagcg aaaaaagatc cattattaaa tatcggtggt ttagtcgcta ttcgtgataa
     1201 tgaagagata tttactttag ccagacaacg ttgtgtaccc atggaaggct ttgtaaccta
     1261 tggtggttta gctggtcgtg atatggctgc aatggtacaa ggtttagaag aaggaactga
     1321 agaagagtat ctgcactatc gtattggtca agttaagtat ttaggtgatc gcttacgtga
     1381 agcgggtatt ccaattcaat atcctactgg tgggcatgcg gttttcgttg actgtaaaaa
     1441 attagttccg caaattccgg gtgatcaatt tcctgctcaa gcagttatta atgcacttta
     1501 tctggagtca ggtgtgcgtg cggtagaaat tggttcattc ttattaggtc gtgatcctgc
     1561 aacgggtgaa caaaaacatg ctgatatgga atttatgcgt ttaactattg cacgccgtgt
     1621 ttataccaat gaccatatgg attatattgc tgatgcatta attgggttaa aagagaagtt
     1681 tgcaacactt aaaggtctag agttcgaata tgaaccacct gtattaagac actttactgc
     1741 aagattaaaa cctatcgaat aataagtaat aatccacaat taaataatgg ctctcgtgca
     1801 atgcgagagc cctatcctta tatatttaag ggatacaaat aatggaaaat acttcagtaa
     1861 ataaagaacc ctcaattatt gtagggggat ttgttctggg tggtgctatg ataggtgcag
     1921 gtatgtttag cctgccaaca ataatgtctg gtgcttggtt tataaactca ctatttatat
     1981 tgtttattgt ttgtttcttt atgttccact cggggatata tattcttgaa tgtatttcta
     2041 aatatggcgc aggaacaaat tactttgata tatccaaaga gttattaccg aagtgggctt
     2101 gttatattgc caatgcttca ttgatctttg tattatatat attgatctat gcttatatct
     2161 ctgcggcggg ttctattatc tatgaagcat cactgttata tggtattaat tttaatctga
     2221 gagctatatt ttttattttt acgatagccc ttggtgctac aatatggtgg ggtggcgctt
     2281 gtgctagccg tttaacctca attttcttat tcattaagat agtattattt atattagcgt
     2341 tttcgggttt gttttttaaa gcaaaaggtg atttattatt tagtgcaact tttgcaggaa
     2401 aaagccaatt atatctttat ccttttattt ttattatcat tccttatgcc attacctcat
     2461 ttggatatca tggtaatgtt tgtagtcttt ataagcttta taatcaaaac gaaagaaaag
     2521 tagttaagag ttgtatcatt ggttgcttgt tagcattagt catctattta ctttggatga
     2581 ttggcactat gggtaattta ccgagagaac aatttattac tattatccaa aaaggcggta
     2641 acttagatgc ctttattgat tcactctata cggtattaaa tagtaaatat attgaaggat
     2701 ttttattatg gttctctatt agtgctgttt tttgctcttt tttaggtgtc gcgataggtc
     2761 tttttgatta tattttagcg tcattaaaat ttaaagataa taaaacaggg cgattgaaat
     2821 caggtgtgct ttgttttaca cctccattgc tattatgtct atttttccct aatggtttct
     2881 taattgctat tgcgtatgca ggcactgctg cttgtgtatg ggccattatt tgtccggcag
     2941 taatggcact gaaagcacga cagaagtttc ctaactcagg ctttaaagta tggggtggta
     3001 aaaagctgat ttatgcagtt attgcttttg gtgttgttgg tattatttgt caatcgtggc
     3061 gcaatttaat ttactgccta tttatcgtta aataagaact caaatcaggg aaaataaaac
     3121 cagagtagca ccattgaata tacgttat
//