From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Paracentrotus lividus mitochondrion, complete genome.
Date: 15 Sep 92 10:05:01 GMT
Distribution: bionet
Checksum: 41635    32

LOCUS       PALMTCG     15696 bp ds-DNA   Circular  ORG       15-SEP-1992
DEFINITION  Paracentrotus lividus mitochondrion, complete genome.
ACCESSION   J04815
KEYWORDS    12S ribosomal RNA; 16S ribosomal RNA; ATPase; F-0-ATPase subunit 6;
            F-0-ATPase subunit 8; NADH dehydrogenase;
            NADH dehydrogenase subunit 1; NADH dehydrogenase subunit 2;
            NADH dehydrogenase subunit 3; NADH dehydrogenase subunit 4;
            NADH dehydrogenase subunit 4L; NADH dehydrogenase subunit 5;
            NADH dehydrogenase subunit 6; complete genome; cytochrome b;
            cytochrome c oxidase; cytochrome c oxidase subunit I;
            cytochrome c oxidase subunit II; cytochrome c oxidase subunit III;
            ribosomal RNA; transfer RNA; transfer RNA-Ala; transfer RNA-Arg;
            transfer RNA-Asn; transfer RNA-Asp; transfer RNA-Cys;
            transfer RNA-Gln; transfer RNA-Glu; transfer RNA-Gly;
            transfer RNA-His; transfer RNA-Ile; transfer RNA-Leu(CTN);
            transfer RNA-Leu(TTR); transfer RNA-Lys; transfer RNA-Met;
            transfer RNA-Phe; transfer RNA-Pro; transfer RNA-Ser(AGN);
            transfer RNA-Ser(TCN); transfer RNA-Thr; transfer RNA-Trp;
            transfer RNA-Tyr; transfer RNA-Val;
            ubiquinol-cytochrome c reductase.
SOURCE      P.lividus mitochondrial DNA.
  ORGANISM  Mitochondrion Paracentrotus lividus
            Eukaryota; Animalia; Eumetazoa; Echinodermata; Echinozoa;
            Echinoidea; Echinacea; Echinoida; Echinidae.
REFERENCE   1  (bases 1 to 15696)
  AUTHORS   Cantatore,P., Roberti,M., Rainaldi,G., Gadaleta,M.N. and Saccone,C.
  TITLE     The complete nucleotide sequence, gene organization, and genetic
            code of the mitochondrial genome of Paracentrotus lividus
  JOURNAL   J. Biol. Chem. 264, 10965-10975 (1989)
  STANDARD  full staff_entry
COMMENT     Draft entry and computer readable copy of sequence [1] kindly
            submitted by M.Roberti 26-JUL-1989.
FEATURES             Location/Qualifiers
     tRNA            1..73
                     /anticodon=(pos:35..37,aa:Phe)
                     /note="Phe-tRNA"
     rRNA            74..956
                     /note="12 S rRNA"
     tRNA            952..1020
                     /anticodon=(pos:982..984,aa:Glu)
                     /note="Glu-tRNA"
     tRNA            1026..1098
                     /anticodon=(pos:1060..1062,aa:Thr)
                     /note="Thr-tRNA"
     tRNA            1231..1299
                     /anticodon=(pos:1261..1263,aa:Pro)
                     /note="Pro-tRNA"
     tRNA            complement(1301..1371)
                     /anticodon=(pos:1337..1339,aa:Gln)
                     /note="Gln-tRNA"
     tRNA            1380..1451
                     /anticodon=(pos:1412..1414,aa:Asn)
                     /note="Asn-tRNA"
     tRNA            1452..1523
                     /anticodon=(pos:1485..1487,aa:Leu)
                     /note="Leu-ctn-tRNA"
     tRNA            complement(1523..1592)
                     /anticodon=(pos:1559..1561,aa:Ala)
                     /note="Ala-tRNA"
     tRNA            1593..1661
                     /anticodon=(pos:1623..1625,aa:Trp)
                     /note="Trp-tRNA"
     tRNA            1662..1729
                     /anticodon=(pos:1692..1694,aa:Cys)
                     /note="Cys-tRNA"
     tRNA            complement(1729..1798)
                     /anticodon=(pos:1765..1767,aa:Val)
                     /note="Val-tRNA"
     tRNA            1812..1884
                     /anticodon=(pos:1845..1847,aa:Met)
                     /note="Met-tRNA"
     tRNA            complement(1885..1953)
                     /anticodon=(pos:1921..1923,aa:Asp)
                     /note="Asp-tRNA"
     tRNA            1966..2036
                     /anticodon=(pos:1998..2000,aa:Tyr)
                     /note="Tyr-tRNA"
     tRNA            2041..2109
                     /anticodon=(pos:2070..2072,aa:Gly)
                     /note="Gly-tRNA"
     tRNA            2112..2184
                     /anticodon=(pos:2145..2147,aa:Leu)
                     /note="Leu-ttr-tRNA"
     CDS             2187..3158
                     /note="NADH dehydrogenase subunit 1"
                     /codon_start=1
                     /translation="MTYLFTVLELLSFLVPVLLAVAFLTLVERKVLGYMQFRKGPKMV
                     GPYGLLQPFADGLKLFIKETLKPSTASPYLFFLSPLLFMALALLLWKLMPVHTPTLNF
                     QLSLLLVLGLSSLSVYAMLGSGWAAKSKYSLMGAMRAVAQTISYEISLALILLSLIMI
                     TRRFNLTYMMNAQEFSWFALRCLPLFYIWFVSTLAETKRAPFDLTEGESEMVSGYNVE
                     YAGGPFALFFIAEYAKMILMNLFSVVIFLGGPWPANELFPLKVLTIGLKTTLLVFLFL
                     WVRAAYPRFRYDQLMFLTWKRYLPLSIGALCATMTMVLTLGIYLPLF"
     tRNA            3160..3230
                     /anticodon=(pos:3191..3193,aa:Ile)
                     /note="Ile-tRNA"
     CDS             3231..4289
                     /note="NADH dehydrogenase subunit 2"
                     /codon_start=1
                     /translation="MHQMMSIFLFLTVVSGTMMVVSAEKWFVMWLGLELRTLALIPIL
                     WFCFTPRKMEATMKYFLVQAFRAALLLKSALMQAWFSGSWSALIPMERFPSLCLRVAL
                     AFNLGLAACHFWLPDVLQGLPFIQGLIIATWQKMAPLFLLFYFNQLKFSYFIMLAALI
                     SILVGGWGGLNQTQTRKILAFSSMGNMGWMVVTSAFSLGTAAMMLFIYLVIKTSIFLI
                     LDFLRMFTLGHLKNTSQLSPMRMTLVILTMLSLGGLPPLTGFILKFSSLYRLMNKGFM
                     FFSSVMMMGSLLRLFFYLRMAFNTTLILFPQHLIRLTAWRNRTEAEPLMAKTWLVSSF
                     SVLRILAIPLTIPLYINV"
     rRNA            4292..5840
                     /note="16 S rRNA"
     CDS             5838..7391
                     /note="cytochrome c oxidase subunit I"
                     /codon_start=1
                     /translation="MQLRRWLFSTNHKDIGTLYLIFGAWAGMVGTAMRVIIRAELAQP
                     GSLLKDDQIYKVVVTAHALVMIFFMVMPIMMGGFGNWLIPLMIGAPDMAFPRMNNMRF
                     WLIPPSFILLLASAGVERGAGTGWTMYPPLSRKIAHAGGSVDLAIFSLHLAGASSILA
                     SINFMTTIINMRTPGMSFDRLPLFVWSVFVTAFLLLLSLPVLAGAITMLLTDRKINTT
                     FFDPAGGGDPILFQHLFWFFGHPEVYILMLPGFGMISHVMAHYSGKREPFGYLGMVYA
                     MMAIGVLGFLVWAHHMFTVGMDVDTRAYFTAATMMMAVPTGIKVFRWMATLQGSNLQW
                     ETPLLWALGFVFLFTLGGLTGIVLANSSMDVVLHDTYYVVAHFHYVLSMGAVFAIFAG
                     FTHWFPLFCGYNLHPLWGKAHFFMMFVGVNLTFFPQHFLGLAGMPRRYSDYPDAYTLW
                     NTVSSMGSTISLVAMLFFIFLIWEAFASQREGVTPEFANASLEWQYNSFPPSHHTFDE
                     TPSTVVMVK"
     tRNA            7401..7469
                     /anticodon=(pos:7431..7433,aa:Arg)
                     /note="Arg-tRNA"
     CDS             7471..7764
                     /note="NADH dehydrogenase subunit 4L (atc start codon)"
                     /codon_start=1
                     /translation="IALLMIILSMFYLGLMGILLNRLHFLSILLCLELLLMSLFMGIA
                     TWKTRKSMPQKTTFNLLLLTLSACEASMGLSLMVALSRTHSSDLVARLRLLQY"
     CDS             7764..8453
                     /note="cytochrome c oxidase subunit II (att start codon)"
                     /codon_start=1
                     /translation="MATWAQFGLQDASSPLMEELTYFHDYALIVLTLITMLVFYGLVS
                     LLLSSRTNRFFLEGQELETIWTVVPAFILIFIALPSLQLLYLMDEVKNPFLTIKAMGH
                     QWYWRYEYTDYKDLEFDSYMVPTSDVSLGKPRLLEVDNRLMLPMQNPIRVLVSSADVL
                     HSWAVPSLGVKMDAVPGRLNQTTFFAARAGLFYGQCSEICGANHSFMPILMESVPFSN
                     FENWVAQYMEE"
     tRNA            8456..8525
                     /anticodon=(pos:8486..8488,aa:Lys)
                     /note="Lys-tRNA"
     CDS             8526..8690
                     /note="F-0-ATPase subunit 8"
                     /codon_start=1
                     /translation="MPQLDFTWWMINFFIIWTAILLTLVILVNNKTAQKLTTTDSLQM
                     EKNRTKWQWL"
     CDS             8675..9373
                     /note="F-0-ATPase subunit 6"
                     /codon_start=1
                     /translation="MTMTMTVRIFGQFFPETLLFIPMNLFSIVFALSWMAFIYPTKWA
                     PSRFQSMWARFRANVLEMIFQKTRPKTAPWAGLMTTVFIVILSAKVLGLFPYAFTATR
                     HMSLTYRLGFPIWMAVKILGFYLAFNRRLSHLVPQGTPRALIPLMVWMETLRLFAQPM
                     ALGLRLAANLTAGHLLIFLLSTAIWLLSSSLMVRRIPMFVIFVLLFMLEMGVACIEAY
                     VFTALVHFYLQQNV"
     CDS             9374..10159
                     /note="cytochrome c oxidase subunit III (att start codon)"
                     /codon_start=1
                     /translation="IMAHQHPYYLVEQSPWPLTGAISGLMMNLGLVLWFHTGKMILLF
                     TGLLLLMLTLVNWWRDMVREATFQGRHTAIVEKGLRYPMILFMTSEVCFFFAFFWAFF
                     HRRLAPAVEIGVTWPPSGMTPLNPFLVPLLKTAVLLSSGVTITWSHHRILAGKRNEAM
                     QALFLTVVLGIYFTMLQAWEYIDAPFTMADRVYGSTFFVATGFHGLHVIMGTTFLLVC
                     LIRLSGHHFSTHHHFGFEAAAWYWHFVDVVWLFLYVCIYWWGS"
     tRNA            complement(10159..10228)
                     /anticodon=(pos:10195..10197,aa:Ser)
                     /note="Ser-tcn-tRNA"
     CDS             10242..10595
                     /note="NADH dehydrogenase subunit 3 (ata start codon)"
                     /codon_start=1
                     /translation="MMTTTIFLFSITSAVAMMFALAAHILPTRNKDGEKKSPYECGFD
                     PLKSARLPFSFRFFLVAMLFLLFDLEMALLFPLPAARFLTHPTTLILISLAFMAILDA
                     GLVFEWMKPPLEWAE"
     CDS             10612..12006
                     /note="NADH dehydrogenase subunit 4 (ata start codon)"
                     /codon_start=1
                     /translation="MMITLMLFTTGIVMTTLVIPQKKLWANAISQRALLSLSSPLLMF
                     SKHWSAPWHNLSNMLASDSISAPLMALSCWLAPMSLMARKKTMRKQSELNQRTFMIMV
                     MFIAGALMMTFSALELLLFYVAFETTLIPTLMLITRWGAQMERFQAGLYFMFYTLFGS
                     LPLLMRLIAMYISSSSLSIPKVELIWTPRETMETLKMWWALSIMAFLIKMPIYGFHLW
                     LPKAHVEAPVAGSMILAAILLKLGGYGLIRLINLFATMSLKSLSLALMTFCSWGALVT
                     RMICVRQTDLKALMAYSSVGHMRMVAAAIFSSTKWGTKGALILMMAYGLVSSDLFSLA
                     KTVYERRGTRTLAMTRGLKTILPLSTLWWLVMSAAKLGLPPSPKLIGEMLMLSSLMAW
                     SIWLFPMMGLATMFGAIYSLMIFQLSQQGTPTKKMNKISLSFSREHLLATLHTLPLML
                     IIINPMSALITWLK"
     tRNA            11997..12064
                     /anticodon=(pos:12026..12028,aa:His)
                     /note="His-tRNA"
     tRNA            12067..12132
                     /anticodon=(pos:12092..12094,aa:Ser)
                     /note="Ser-agn-tRNA"
     CDS             12134..14050
                     /note="NADH dehydrogenase subunit 5"
                     /codon_start=1
                     /translation="MVMNPSTMVSTLRMRMMAILAVSMFFFSKSYFYPPQNKKLVRFR
                     VSKDTLRNKPNGRRLDYKRGPFAMRVLKTLAFLSVMSLLISVQKRFKSMKLTFSLWFK
                     KTPTNVSMNFLLDQYFLLFLSVGLMVTWSMMEFSYYYMKEDPKGKAFFRPFGHFSLKM
                     LILTSSKRLFLVFLGWEGVGFLSFLLMRWWATRKDARRSALEAVMYNRIGDMGLMTFM
                     SLAVLTTKSWNLTEMMTKEPKNHFVLFMLFGLILAAAGKSAQFGLHPWLPAAMEGPTP
                     VSALLHRSTMVVAGVFLLVRTSELFSNYPNAKTMVLVLGGTTALFAASTAMAQHDMKK
                     IMAYSTTRQLGLMVAAMGMGQPVLAFFHMCTHAFFKAMLFLCLGRVMHSLKNERNLRK
                     MEGMRDLSPVTSACLAMGRLALMGTPFLAGFYSKDLILEAARARFAKTLGISLGMVAT
                     MLTTVYRFRIVFFCFSSNKSISPLSPIGEEKRNLMNALLRLSIGTILRGWFFSNFIFS
                     PPSFTVTPEEKGLPLLVTVVGLTVIFISLRYLSSNPMKSTSHSTTTSQWFFVDIVHSA
                     LTTTSFISSSFSSSRTLDRGWQENIGAQGMAQSSTALSKANQARQMGLMKRYIASSIA
                     AMTMMATLTFMVLS"
     CDS             complement(14051..14533)
                     /note="NADH dehydrogenase subunit 6"
                     /codon_start=1
                     /translation="MALYLILVLMLFGRTLVFYRLSPYYSALGLVVVSISGCIILSFL
                     GGSFVALVLVLVYMGGMLVVFAYSSAISAERFPSVNNLGEVIGLSVLFSSWVFLSFDN
                     FQDFSKSFSCFMSGERLVEAGFYMKGFLVLVGVFVLLVVLVGALMISRGIEKTVIRAI
                     "
     CDS             14556..15695
                     /product="apoprotein"
                     /gene="cytochrome b"
                     /codon_start=1
                     /translation="MLGPLRKEHPIFRILKRTFVDLPLPSKLSIWWKFGSLLGLCLMT
                     QILTGLFLAMHYTADISLAFSSARHMCRDVNYGWLLRKVHAKGASLFFICMYCHMGRG
                     LYYGGSNKMETWKVGVILFLVTVLTAFVGYVLVWGRMSFWAATVIANLVTAVPCVGTT
                     IVQWLWGGFSVDNATLTRFFAFHFLFPFIMAALAMIDLVFLHNSGANNPVGLKSNYDK
                     APFHIYYTTKDTVGFMALIAALFVLALLFPCALKDPEKFIPANPLSHPPHMQPEWYFL
                     FAYAILRSIPNKLGGVMALVAAMLVLFLMPLLKTSKKESNSFRPLSQATFWMLVATFF
                     VLTWMGSQPVEQPFVLMGQMASLLYFSLFMFGFPLVSSLEKKMMFS"
                     /partial
BASE COUNT     4829 a   3533 c   2697 g   4637 t
ORIGIN
        1 gcaaaggtag cttaacagat taaagcatag cactgaaaat gcttcaaaga gagttaaagt
       61 ccctccctta gcaaccgatt tggtcctagt cctaacttta gctaattttt agttgccaca
      121 tgcaagccca atgacaaacc agtgaagtaa aaattttggt ctccccacct aaaaagagac
      181 aggagcaaaa attctagtat caggccctaa ccaatgccca cgacactaag caatacgcca
      241 caactgcagt actaaacatt agacaatcgg aaaaatccgg atctagcaaa aaaataagca
      301 agggggcaaa atacgtgcca gccaccgcgg ttatacgtac ttcctaaagc taaataaaac
      361 ggtataaagg gcggttagaa aaatagattt gactgtagct cccagctagt ggtaaaaaac
      421 taatctgaga agaaacataa gtcaaaatat ttatttgaac ccgcgaaaac taagacctaa
      481 accaggatta gataccctac tatacttaga cgtaaacaac ctaagcacca gagaactaca
      541 aactaaaagt ttaaaactca aaggacttgg cggttttcca tacctccctg gagagcttgc
      601 cattgaatcg ataatccacg aaacacctca ccaacttttg taaatcagct tgtataccat
      661 cgtcgtaagt ctacttctta agaaagttgg ctcctaggga aaattcccag acgtcagatc
      721 aaggtgcagc acataagctg ggaatgagtg agctacaatg tttttaacga ccagcgaaaa
      781 tagaaatgaa acaattcttt agaagttgga ttcagcagta agccccaaat agaaaatggg
      841 gctgaaaaga gctctggaat gcgtacacat cgcccgtcac tctcgcctag cttaacttaa
      901 gtaaggggag aaaagtcgta acacaatagg cacaccggaa gcctggaaaa tgctcctata
      961 gttgaactac aacaagagct tttcacgctc taagtttgag ttaaaatctc aatagaagct
     1021 aacaaacctt gaaggctcaa tagttagagc gccaagtctt gtaaacttgg agagagggtt
     1081 aaagtccctc tcaaggcttt tgtatttccc atcacaacat gtccccctct ctcctcgtcc
     1141 gtcggtcggg cgtttctgta ttaccttttt tcttatgggg gggggggggg gggggggggg
     1201 ggatttatat ttatatataa cacacgttac caaagaatag tttaagaaaa acaacagctt
     1261 tgggagttgt agacgtaagt gaaaacctta cttctttgaa ttaaggagat gggaattgaa
     1321 cccacaacaa ggaagtcaaa gttcctcgtt tttccattaa actactcctt agaagaaact
     1381 gggttgtagc taaatgtaaa ggcgcttggc cgttaaccaa gaaatagtag gataaaaacc
     1441 tatcttccca ggctgaaata gcaaagtggt taatgcaaaa gacttaggat ttttcatcga
     1501 aggttcaact ccttctttca gctgtagttc ctaagaattt aacttaggat ttctgcttgc
     1561 aaagcaaacg tttttcacta aactagaacc acaagagctt aagttaaata aactgaaagc
     1621 cttcaaagct ttttataaga atggaaactt cttagctctt gggctttgta gtgtaaataa
     1681 cattttggat tgcaaatcct tagatgcaat tagttcattg ccaaagcttc aagacaaccc
     1741 gaattgcacg agtgtgacct ccgtgtaaaa gaggaactta ctaactagct atgtcttgta
     1801 tatatatata tagcagagta agctaagtgc taagcttttg ggctcatacc ccaagaatgg
     1861 aaggatagaa acctccctct gcttcagaag ccgctggaat ttaaccagca attttggcct
     1921 gacaacccaa agttatattt taactagacc tcttctttcc tttttaataa gatggctgag
     1981 agaacaagcg gtggattgta aatccataca caaaggttaa atcccttttc ttattatctt
     2041 actctatgag tacaacagta cctttgactt ccaatcaaat agctcttagc gaaaatctaa
     2101 gatagagtat ggctaaaata gcaaagtggt taatgcagaa ggcctaagac cttcctatca
     2161 aaggttcaac tccctttttt agctcgatga cttatttatt tacagtacta gaactacttt
     2221 cttttttggt gccagttctt cttgcagtag ctttcctcac tctagttgaa cgaaaggtat
     2281 tgggttacat gcaattccga aagggaccaa aaatagttgg accctatgga ctcttacaac
     2341 cctttgcaga cggccttaag ctttttatta aggaaacttt aaagccctcc acagcatcac
     2401 catacttgtt ttttttgtct cccctgttat ttatggcact tgcattgctc ttgtgaaaat
     2461 tgatgcctgt acacacacca accttaaatt tccaactgtc tctgctacta gtccttggac
     2521 tgtcaagctt atccgtttat gccatactag gatctggatg ggccgccaaa tctaagtact
     2581 cgttaatagg agcgatacga gccgtagccc aaactatttc ctacgagatt agcctcgcat
     2641 taatcttact ttcacttatt ataattacca gaaggtttaa cttaacatat ataatgaacg
     2701 cccaagagtt ttcctggttt gcactaagat gccttccact gttttatatt tgatttgtct
     2761 caaccctagc agagacaaaa cgtgccccct ttgatctaac agaaggagag tctgagatag
     2821 tatcgggata caacgttgaa tatgcaggag gacctttcgc cctgttcttt attgctgaat
     2881 atgctaaaat aattttaatg aatctctttt cagtggtcat ttttcttggt ggcccatgac
     2941 cggcaaacga actatttccc ctaaaagttt taactattgg attgaagacc actcttttag
     3001 tttttttatt cctatgagta cgggctgcct atccccgatt tcggtatgat caactaatgt
     3061 ttctaacttg aaagaggtac ctaccactct ccattggagc cctatgcgcc acaataacta
     3121 tagtactgac cctaggaatt tatcttcccc tattttaaag agcttgctcc caaagcttgg
     3181 gtgtctagct gataattaga ctatcaaggg ttaaattccc ttcaagctct atgcaccaaa
     3241 tgatatctat ctttttgttt ttgacagtcg tctctggaac tataatagtc gtttcagcag
     3301 aaaaatgatt cgttatatgg ctgggactag aactaagaac cctggcctta atcccaatcc
     3361 tttggttctg cttcacgccc cgaaaaatag aagcaaccat aaagtacttc cttgttcaag
     3421 cttttagagc cgctctccta ttaaaaagtg ccctaataca agcctgattt tccggatctt
     3481 gatcagccct aattccaatg gaaagattcc cctccctctg ccttagagta gcactagcct
     3541 tcaatttggg actagctgcc tgtcactttt ggctcccaga tgtacttcaa ggtctgccat
     3601 ttattcaagg actgattatt gctacgtgac agaagatagc tcctctcttt ctgctattct
     3661 attttaacca actaaaattt tcctatttca ttatactagc ggcgctaatt tcaattttgg
     3721 ttgggggatg aggagggtta aaccaaacac aaacacgaaa gattctagct ttttcctcta
     3781 taggaaatat gggatgaata gtagtaacct ccgctttctc actgggcact gcagccatga
     3841 tgctttttat ttacttagta attaaaactt ctatcttttt aatcttagac tttttaagaa
     3901 tatttacatt agggcactta aaaaatacct cacaactatc accaataaga ataaccttag
     3961 ttatccttac aatactatca ctgggcggtc ttcctccatt aacaggattt attctgaagt
     4021 tttcctccct ttatagacta ataaataaag ggtttatatt cttctcatct gtcatgataa
     4081 taggaagcct tttaagactt ttcttttacc tccgaatagc ctttaacacc accctaattc
     4141 tcttccctca gcatttgatc aggctaaccg cctgacggaa caggacagaa gcagaacctc
     4201 ttatggccaa gacatgatta gtttcatcct tttcagtcct aagaatttta gccatccctt
     4261 taactattcc tttatatatt aatgtataga aaaattatta ccataggcct ccccaaaata
     4321 aaaatattta gctttctttg taaaaaaaac actttcacta ttaaagtata accactagaa
     4381 attttttaaa ggaccaacag aaaaagtacc gaaaggaata atgaaataat actaacttaa
     4441 taaatattaa gaaggaaaga ttattccttt taccttgtgt ataatggatt aacgagacta
     4501 cccaaaaaac tatttgacac ccgaaactgg acgagctaat cttctcctct ttttagaaga
     4561 tttatcctta ctgttgcaat agtaagagaa aagggaagat tagatgtgaa atgctaaccg
     4621 cgcccagaga tagctggttc tccaagaaat tagttttagc taagccttta aaacaaaaat
     4681 agaattactt taacatataa aaccaaaaag attggttttt ttttaagttg aaggtcaggg
     4741 ctttagggat aagcctagag cccaaaatgg aaaaccccaa ttctgataga aaaagacaat
     4801 aaccacataa atagttgccc ctaaagaagt aggcttagaa gcagccacct aaataaaaag
     4861 cgttaaagct taattaaaaa acctcaacta aaaaatcctt ggcacaacct ctgaatctac
     4921 agaaaaatat tggagccctc tgattctcag aagagacaat gttaatatga gtaagaatac
     4981 tttcacctgc gttttatacc cccctaacct tggaaataaa aacaatgaga ctaaaaaaat
     5041 tgttttaact attgtcctcc aactcaggag aaagaaaatt aataaattgg aaaaaaggaa
     5101 ctcggcaaaa cacagtttcg cctgtttacc aaaaacatcg ctccccgata aataacaaca
     5161 tgaggagtcc tgcctgccca gtgactaaag gttaaacggc cgcggtatct tgaccgtgcg
     5221 aaggtagcat aatcatttgt cttctaaata aagactagta tgaatggcaa gacggaactg
     5281 aactgtctct tttccaaata atccgaattt cacttctccg tgaagaggcg gagggtaaaa
     5341 tcgttagacg agaagaccct gtcgagcttt tagcaaaaac ttaactagta aaacacttaa
     5401 actactctag taaatgaatg taagtataac taagctacta agattaagtg aagctttggt
     5461 tggggcaacc gcggagtaaa agaagcctcc gctaaaaaaa tattaaccac taaactaagg
     5521 actaccatcc tataatcatg aatgattaaa ttgatccatt tagatgacca aaggaacaag
     5581 ttaccgcagg ataacagcgt tatcttttct gagagttcac attgacgaaa aggtttgcga
     5641 cctcgatgtt ggatcgggac atcctaaagg tgcagaagct tttaagggtt ggtctgttcg
     5701 accattaaag tcctacgtga tctgagttca gaccggcgaa agcaggtcag tttctatcta
     5761 cgtatttatt ctcctcagta cgaaaggacc aggataagag tgcccatgca gacatgtatt
     5821 gcacttcaat taaaaacatg caactaagac gatgactatt ttctactaat cacaaggaca
     5881 ttggaacact ttatttaatt tttggtgcct gagcaggcat ggtaggaact gcaatgagag
     5941 taattatccg agccgaacta gcacaaccag ggtctttatt gaaagacgac caaatttaca
     6001 aagtagtagt tactgcacac gcactagtaa tgattttttt tatggtaatg ccaattatga
     6061 taggaggatt tggaaactga cttatcccac taatgattgg agcaccagat atggccttcc
     6121 cccgaatgaa caatatgaga ttctggttaa tccccccttc ttttattttg cttctagcct
     6181 cagcaggagt agaaagagga gccgggacag gatgaaccat atacccccct ctctccagaa
     6241 aaattgccca cgcaggaggc tcagtagact tagcaatttt ctcactccat cttgcgggtg
     6301 cctcttctat cctagcctca attaacttta taaccacaat tattaatatg cgaacgccgg
     6361 gaatgtcttt tgaccgacta cctttgtttg tttggtccgt ttttgttaca gcattcctac
     6421 ttcttctttc tctaccagta ctagcaggag cgattactat gcttctaaca gaccgtaaaa
     6481 ttaacacaac atttttcgac ccagcaggag gaggagaccc aattctgttt caacatttat
     6541 tctgattttt tggtcacccc gaagtatata ttctaatact acccggattt ggaatgattt
     6601 cacacgtaat agcccactac tcagggaagc gagaaccttt tggatacctg ggaatggtct
     6661 acgccatgat agcgatcgga gtccttggat tcctagtttg agctcaccat atgtttacag
     6721 ttggaatgga cgtagacaca cgagcgtact ttacagctgc cacaatgata atagcagtac
     6781 ccacaggaat taaggtattt agatgaatgg caacccttca aggatctaac ctacaatgag
     6841 aaactcctct actatgagcg ctcggatttg tcttcttatt caccctaggc ggacttactg
     6901 gaattgttct agcaaattct tctatagacg tagtacttca tgacacatac tacgttgttg
     6961 cccactttca ctatgtatta tcaatgggtg cagtattcgc tatttttgct gggtttactc
     7021 actggttccc actattttgc ggatataact tacaccctct atgaggaaag gctcactttt
     7081 ttatgatgtt cgttggggtc aacctaacat tcttccccca acatttccta ggtctggctg
     7141 gtatgccacg gcgatactca gactacccag atgcctatac cctctgaaac acggtttcat
     7201 ctataggatc aactatttct ttggtagcca tgctattctt tattttccta atttgagaag
     7261 ctttcgcctc tcaacgggaa ggggttaccc cagaatttgc taatgcctcc ctggagtggc
     7321 aatacaactc attcccccca tcccaccaca catttgacga aacaccttct accgtagtga
     7381 tagttaagta aaagtaattg tgagagttag tttaagaaga acccctgatt tcggctcaga
     7441 aggctttggt taaagtccaa aactctttat atcgccctac ttataattat tttatcaatg
     7501 ttttacctag gattaatggg aattctctta aaccgactac attttttatc tattcttttg
     7561 tgcctagagt tattactaat atcgctattt ataggaattg ctacctgaaa aaccaggaaa
     7621 tcaatacccc aaaaaactac atttaacctt cttttattga cactatctgc ttgtgaagca
     7681 agcataggac tctcccttat ggtagcactc tcccgaaccc actcttccga tctagtggca
     7741 agactaagac ttctccaata ttaatggcaa cttgagcaca atttggtcta caagatgcgt
     7801 cctccccact aatggaggag ctaacatact ttcacgatta cgccttaatc gtacttaccc
     7861 tcattacaat actggtattt tacgggctag tctcattact actatcttcc aggactaatc
     7921 gattttttct tgaaggccaa gaactagaga ctatttgaac tgtcgttcct gcttttattc
     7981 ttatcttcat cgcacttccc tccttacaac tactttacct tatggatgaa gttaaaaatc
     8041 cttttttgac aattaaggca ataggacacc aatgatattg aagatatgaa tacaccgact
     8101 ataaagacct agagtttgac tcatacatgg tccctacttc cgatgtttct ctaggcaaac
     8161 cccgacttct agaagtagac aatcgactaa tactacccat gcaaaaccct atccgagtct
     8221 tagtttcctc agcagacgta ttgcactcat gagctgttcc ctccttagga gtaaagatgg
     8281 atgcagtccc aggccgactt aatcaaacca ccttctttgc agctcgagca ggactgttct
     8341 atggacaatg ctctgaaatt tgcggagcaa atcatagttt catgcccatt cttatagaat
     8401 ccgtcccttt ctctaatttc gaaaactggg ttgcccaata catagaagaa taaaccctta
     8461 attagcttat ttaaagccct aaactcttaa tttaaaagaa aatagttaaa acctattatt
     8521 aaggagtgcc acaactagat tttacttgat ggataattaa tttttttatt atttgaactg
     8581 caatcttact aactcttgta attttggtta acaacaagac agcccaaaaa ttaactacaa
     8641 cagactcttt acaaatagaa aagaatagaa caaaatgaca atgactataa ctgtaagaat
     8701 ttttggtcag ttctttcccg aaaccctact gtttattcca atgaatttgt tttcaattgt
     8761 gtttgctctg tcttgaatag catttattta ccctacaaaa tgagccccat cccgattcca
     8821 atccatatga gctaggtttc gtgctaatgt cttagagatg attttccaaa aaacaaggcc
     8881 caaaaccgcc ccatgagccg gcctaataac tacagtattc atcgttattt tatctgccaa
     8941 agttctaggc ttatttcctt atgctttcac tgctacgaga cacatatccc taacctatag
     9001 attaggattc cctatctgaa tggccgtaaa aattcttggg ttttacctgg catttaacag
     9061 acgactaagc cacctagttc cacaaggaac ccctagagca ctaattcccc taatggtctg
     9121 aatagaaacc ctaagactat ttgctcaacc tatagctctc ggactccggc tcgccgccaa
     9181 ccttacagcc ggccatctat tgatttttct cctttctact gctatttgac ttctatcttc
     9241 tagcttgatg gtaagaagaa ttccaatatt cgtaatcttc gttttactat tcatactaga
     9301 aataggagta gcctgcattg aagcatacgt gtttaccgct ctggttcact tttacctcca
     9361 acagaatgtt taaattatgg ctcatcaaca cccatactat ttagtagaac aaagcccatg
     9421 acccctaact ggggctatta gtggcttaat gatgaacctc ggcctagtcc tatgatttca
     9481 taccggaaaa ataatcttac tatttacggg acttttgctt ctaatattaa cattggttaa
     9541 ctgatgacga gacatagtac gagaagctac attccaggga agacacacag caattgtaga
     9601 aaaaggtctt cgatatccta tgatcctttt tataacctcc gaagtatgct tcttttttgc
     9661 tttcttctga gccttttttc acagaagact ggcaccagca gttgaaattg gagttacttg
     9721 accaccctca ggaataacac ctctcaaccc ctttttagtc ccccttctta aaactgccgt
     9781 cctgctatcc tcaggagtta ccattacatg gtcccaccac agaattctag ccggaaaacg
     9841 aaatgaagcc atacaagcac tattcctaac agttgtccta ggaatttact tcactatact
     9901 acaagcatga gaatatattg atgccccatt tacaatagcc gacagggtct atggctctac
     9961 tttttttgta gcgacaggat tccatggcct tcatgtaatc ataggaacaa cttttttact
    10021 agtatgctta attcgactat cgggacacca cttttcaacc catcaccact ttggttttga
    10081 ggccgctgct tgatattgac attttgtaga cgtagtatga ctcttcttgt atgtctgcat
    10141 ttactgatga ggatcttaaa gagaagagag gaattgaacc tctatccaac ggtttcaagc
    10201 cgcatgcatt tctatctgcc acttctccta atcatatata aataatgaca acaacaatct
    10261 ttttattttc aattacctcc gccgtagcaa taatatttgc cttagcagca cacatcttac
    10321 caacccgaaa caaagacggg gagaagaaat ctccctacga atgtggattt gacccgctaa
    10381 aatcagctcg tcttccattt tcttttcggt ttttcctggt cgctatacta ttcctgctgt
    10441 ttgatctaga aatagcacta ttattcccac ttccagcagc aagattccta acccacccaa
    10501 ctactttaat ccttatttcc ttagcattca tggccattct agatgccggg ctagtctttg
    10561 aatgaataaa accaccacta gagtgagctg aatagcttct gatttgttaa aataatgatt
    10621 actctaatat tatttaccac cggaattgta atgaccacgt tagtgattcc ccaaaaaaag
    10681 ctatgagcta acgctatttc acaaagagct ttactctctc tctcttctcc cttactgata
    10741 ttttcaaaac attgatctgc cccttgacat aacttatcta acatactagc ttcggactct
    10801 atttcagcac ccctaatagc actaagctgt tgactggccc caatatccct tatagcaagg
    10861 aaaaaaacca taagaaagca aagtgaattg aatcaacgaa cttttataat tatagtaata
    10921 ttcattgcgg gtgctttgat aataacgttc tctgccctag aactactctt gttttacgtt
    10981 gcttttgaaa caaccttaat tcccacactt atactaatta cacgatgagg agcacagatg
    11041 gaacgattcc aagcagggct ttatttcatg ttctacactt tattcgggtc ccttccactc
    11101 cttataaggc ttatcgcgat atatatctca agttcctctc tctctattcc aaaagtcgag
    11161 ctaatctgaa cacccagaga gaccatggaa accctaaaaa tatggtgagc cctatcaatc
    11221 atagcatttt taattaagat gccaatctat gggtttcacc tatgactacc aaaggctcac
    11281 gtggaggccc cagtagcggg atccatgatt ctagctgcca tcctattgaa gttaggggga
    11341 tatggcctta ttcgactaat taatctgttt gccactatat cactaaaatc cctctctctt
    11401 gctctgataa cattctgctc ttggggggca ttggtaacaa gaataatttg tgtgcggcaa
    11461 acagacctaa aggcgcttat agcctactct tcagttggcc acatgagaat agtagcagca
    11521 gctatctttt cttcaaccaa atgaggaaca aaaggagcac taatcctaat gatagcatac
    11581 ggcctagttt cctcagacct attctcctta gctaaaactg tctatgagcg aagaggaaca
    11641 cgaaccttag ctataacccg aggactgaag acaattttac ccctaagcac cctctggtgg
    11701 ttagtaatgt ccgcagcgaa attaggacta ccgccctcac ccaaactaat cggggaaata
    11761 cttatactgt cctctctaat agcctgatct atttggctgt tcccaataat aggcctagca
    11821 acaatatttg gtgcaatcta ttcactaatg atttttcaac tgtcacaaca gggaacccca
    11881 accaaaaaaa taaataaaat ttccctttct ttttcccggg agcatctgct agccaccctt
    11941 cacacacttc ccctaatact gattattatt aaccccatat cagcccttat cacatgacta
    12001 aagtagttta taaaaacacc agcctgtgga actggagtaa ctggttaaaa cccggtctta
    12061 agtccgaaat ctattggttt accttagtcc tgctaagtct aaaggtctgc ggttcaactc
    12121 cgttgagttt tcgatggtaa taaacccatc aactatagta tctacactta gaatgagaat
    12181 aatggctatt cttgcagtga gtatattttt cttctccaag tcctactttt acccacccca
    12241 aaacaaaaaa ttagtaagat tccgagtatc aaaagatacc ctaagaaata aacccaacgg
    12301 aagaagactt gactataaaa gaggcccctt tgccataaga gtcctcaaga ccctggcttt
    12361 tctatccgtc atatctctac tcatttcagt gcaaaaaaga tttaagtcca taaaactaac
    12421 attttcccta tgatttaaaa aaaccccgac taacgtgtca ataaactttc tgcttgacca
    12481 gtattttcta ctttttttat ccgttggact aatagtaacc tgatcaataa tggaattttc
    12541 ttactactac atgaaggagg accctaaagg aaaggccttc ttccggcctt ttggccattt
    12601 ttctcttaaa atgttaattt tgacttcatc aaaaagacta ttcttagtat ttttgggatg
    12661 agagggagta gggttccttt cttttttact cataagatga tgagcaaccc gaaaagacgc
    12721 aaggagatcc gccctcgagg ctgtaatata caaccggatt ggagatatag gactgataac
    12781 ttttatgtca cttgcagttc tcacgaccaa atcttgaaac ctaacggaaa taataacgaa
    12841 agaacctaaa aatcactttg ttctatttat gttgttcggt ctaattttag ctgccgctgg
    12901 taagtcggcc caattcggcc ttcacccatg acttcccgca gcaatggaag ggccgacccc
    12961 agtctcagcc ctacttcaca ggtcaaccat ggttgtcgca ggggtatttc tactggtacg
    13021 aaccagcgag ctgttctcta attaccctaa tgcaaaaacc atagttttag tcttgggagg
    13081 gactaccgct ttatttgctg cctcaactgc aatagcccaa cacgacataa agaagattat
    13141 agcatactca acaacaagac agcttggttt aatggtagct gcaataggaa taggacagcc
    13201 agtattagca tttttccaca tatgcaccca tgcatttttt aaggcaatgc tatttctctg
    13261 cttaggaaga gtgatacaca gcttgaaaaa tgagcgaaac cttcgaaaga tggaagggat
    13321 aagagacctt tcaccagtta catcagcttg cttagccatg ggaagactgg ctttaatggg
    13381 aaccccattt ctggcagggt tttactcaaa ggatcttatt ttagaagcag cgagagcgag
    13441 atttgcgaaa acactgggaa tttcgttagg aatagttgca acaatgctaa ctaccgtata
    13501 tagattccga attgttttct tctgtttctc atcaaacaaa tctatttccc ccctttctcc
    13561 tattggagaa gaaaaaagaa acttaataaa tgccctctta cgactttcta tcggcacaat
    13621 tttaagaggc tgattcttct ctaattttat cttctcccct ccttccttta ccgttacacc
    13681 cgaggaaaag ggtctccctc tcttagtaac tgtagttggc ctaacggtaa tttttatctc
    13741 cctaagatac ttatcctcca accccataaa gagcacctct cactcaacaa caacatctca
    13801 gtggttcttt gtcgatattg tccactcggc actcacaact acttctttca tttcctcctc
    13861 attctcatcc tcacgaacac tagatcgggg gtggcaagaa aatatcggcg cacaaggaat
    13921 agctcagtct tcgactgctc tatccaaggc gaaccaagcc agacaaatag gactaataaa
    13981 gcgctacatt gcctcatcaa ttgcagctat aaccataata gcaaccctta cttttatagt
    14041 cttatcatag ctaaattgca cgaatgactg ttttttcaat tccacgagaa attattaaag
    14101 ctcctaccaa aaccacaaga agaacaaata caccaactaa gactaaaaac cccttcatgt
    14161 aaaagcctgc ctccactaac ctttctccac ttataaagca agagaaagat ttactaaaat
    14221 cttgaaagtt atcaaaagac aagaataccc aagaggaaaa taagacagat agcccaatca
    14281 cctcccccaa gttattaact gaggggaatc gctccgctga gatagcacta gaataggcaa
    14341 aaaccactag cattccaccc atatagacaa ggaccaagac aagagctacg aaagagccac
    14401 ccaaaaaaga taagatgata caaccagaaa tagacacaac tactagccca agagcagaat
    14461 aatagggaga caacctgtaa aataccaagg ttctcccgaa aagcattaac acaagaatta
    14521 aatacaacgc cattaattat atataacttt aaattatgct aggtccatta cgaaaggaac
    14581 accccatttt ccgaatctta aaaagaacat ttgttgacct cccacttccc tccaaacttt
    14641 ccatctggtg aaaattcggc tctttactag gactatgctt gataacacag attttgaccg
    14701 gactatttct agcaatgcac tacacagccg atatctccct agcattttcg tcagccagac
    14761 acatatgccg agatgtgaat tacgggtgac tgcttcgaaa agttcatgca aaaggagcct
    14821 ccctattttt tatctgcatg tattgccaca tagggcgagg actctactac ggaggatcca
    14881 ataagataga aacctgaaaa gtaggagtga ttttgttcct agtgaccgta cttactgcct
    14941 ttgtgggata cgtattagtc tgagggcgaa tgtctttttg agccgcaact gtgattgcga
    15001 acctagtcac cgctgttccc tgtgtaggga caacaattgt ccaatggttg tgaggaggat
    15061 tttcagtaga caatgccacc ctaactcggt tttttgcttt tcactttcta ttccctttta
    15121 ttatagcagc attggccata attgacttag tatttctcca caatagcgga gccaacaacc
    15181 cagtagggct gaaaagtaat tatgataagg ccccatttca catttattat acaacaaagg
    15241 acactgtagg attcatagcc ctgattgcag cgctatttgt cctagctctc ttatttcctt
    15301 gtgccttaaa agaccccgaa aaatttatcc ccgcaaatcc cctatcgcat cccccacaca
    15361 tacaaccgga atggtacttt ttatttgcct atgccattct acgatctatc ccaaataagc
    15421 tagggggagt tatagcatta gtagcagcca tactagtact ctttttgatg ccgttactca
    15481 aaacatctaa gaaagagtca aattcattcc gtcccctatc tcaagcgacc ttttgaatat
    15541 tagtggctac ttttttcgta ctaacatgaa taggaagcca acctgtagaa cagccgttcg
    15601 ttcttatagg acaaatagcc tcactgcttt actttagtct atttatattt ggctttccct
    15661 tagtttcttc tctagagaaa aagatgatat tttcat
//