From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: A.eutrophus nosZ gene encoding nitrous oxide reductase.
Date: 15 Aug 92 10:01:06 GMT
Distribution: bionet
Checksum: 37585     7

LOCUS       AFANOSZG     2442 bp ds-DNA             BCT       15-AUG-1992
DEFINITION  A.eutrophus nosZ gene encoding nitrous oxide reductase.
ACCESSION   X65278
KEYWORDS    FNR binding; Nitrous oxide reductase; nosZ gene.
SOURCE      Alcaligenes eutrophus DNA.
  ORGANISM  Alcaligenes eutrophus
            Prokaryotae; Gracilicutes; Scotobacteria; Aerobic rods and cocci;
            Alcaligenaceae.
REFERENCE   1  (bases 1 to 2442)
  AUTHORS   Zumft,W.G.
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
REFERENCE   2  (bases 1 to 2442)
  AUTHORS   Zumft,W.G., Dreusch,A., Loechelt,S., Cuypers,H., Friedrich,B. and
            Schneider,B.J.
  TITLE     Derived amino acid sequences of the nosZ genes (respiratory N2O
            reductase) from Alcaligenes eutrophus, Pseudomonas aeruginosa, and
            Pseudomonas stutzeri reveal potential copper-binding residues.
            Implic
  JOURNAL   Eur. J. Biochem. (1992) In press
  STANDARD  full automatic
COMMENT     *source: strain=H16, ATCC 17699;
            From EMBL    entry AENOSZG;  dated 06-AUG-1992.
FEATURES             Location/Qualifiers
     protein_bind    119..132
                     /function="Transcriptional activator"
                     /bound_moiety="FNR protein"
                     /label=FNR-box
     RBS             206..210
     CDS             227..2158
                     /function="Respiratory oxidoreductase ofdenitrification"
                     /EC_number="1.7.99.6"
                     /product="nitrous-oxide reductase"
                     /gene="nosZ"
                     /codon_start=1
                     /translation="MSKEKASIGNGPGGIGRRQFLGTAALAGLAGVVACTDKGAAPAA
                     AAVGAPASGAHGAAHGAGASVHLKPGELDTYYGLWSGGHTGDMRVLGLPSGREILRIP
                     CFVPDALVGWGITNESKKVMGARPDGTLRYTVADTHHTHASYKDGNYDGRYAWVNDKI
                     NSRIARIRLDYFICDKITELPNVQGFHGIFPDKRDPVDTKINYTTRVFCGGEFGIPLP
                     SAPTEDAGKYRSLFTCVDAETMAVRWQVLIDGNCDLVATSYDGKLAATNQYNTENGAH
                     FEDMMSAERDACVFFNIARIEAAVQAGKFKTYGDSKVPVVDGTQAANKDPKTALTAYV
                     SVPKNPHGVNASPDQKYFICAGKLSPTATVIELSRVLGWFDGKQEKLDDAIVAEVELG
                     LGPLHTAFDGRGNAYTTLFLDSQLVKWNLDAAIKFHKGDKNAKYVVDRLDLQYQPGHV
                     NASQSETVAADGKYLAVGCKFSKDRFLPVGPLHPENEQLIDISGQKMVLMADHPVRGE
                     PHDFIIFKRELVRPKQVYALDDFPLAIKDPKESGVFRNGRKVTVKITSQAPAFSLREF
                     KLKKGDEVTLILTNLDKIEDLTHGFAIPKYNVNFIVNPQETASVTFVADKPGVFWCYC
                     THFCHALHLEMRTRMIVEA"
                     /citation=[2]
                     /citation=[1]
     misc_feature    1982..2131
                     /function="Putative copper-binding site"
                     /note="This domain showsstructural similarity to the
                     putative CuA-bindingsite of the subunit II of cytochrome c
                     oxidase, EC1.9.3.1"
                     /label=CuA-site
     stem_loop       2169..2203
                     /function="Putative transcriptional terminator"
BASE COUNT      465 a    781 c    765 g    431 t
ORIGIN
        1 cttcctcttg ctcatcagat gctccgtttg tgtggcatcc attctaagaa gcagaggcgt
       61 gcagtggttg cacggtgccg gccttattca tgcatttttc tacttgtttt gttgcctttt
      121 gattcgagtc aagttcagac tggtccggaa cttcgaagat tcgcctgttc acaaacatct
      181 gcaaaacagc cggtgattcc ggcacatatc tcttcgaggg ttctccatga gcaaagagaa
      241 ggcatcgatc ggcaacggtc caggcggtat cggtcgccgc cagtttctcg gcacggcagc
      301 gctggccggc ctagcggggg tggtcgcctg cacagacaag ggcgccgcgc ccgcggcggc
      361 ggccgttggc gcaccggcaa gcggggcaca cggggccgcg cacggcgcag gcgcgtccgt
      421 gcacctcaag cccggggaac tggatacgta ctatggcctc tggagcggcg ggcataccgg
      481 cgacatgcgc gtgctggggc tgccgtcggg gcgcgagatc ctgcgcatcc cgtgcttcgt
      541 gcccgatgcg ctggtcggct ggggcattac caatgaatcg aagaaggtga tgggcgcccg
      601 gccggacggc acgctgcgct acacggtggc cgatacccac catacccatg cttcgtacaa
      661 ggacggcaac tacgacggcc gctatgcctg ggtcaacgac aagatcaatt cccgcatcgc
      721 gcgcatccgc ctcgactact tcatctgcga caagatcacc gagttgccca atgtgcaggg
      781 cttccacggc atcttcccgg acaagcggga tccggtcgac acgaagatca actacaccac
      841 acgcgtgttc tgcggcggcg agtttggcat tccgctgccc agtgcgccga ccgaggatgc
      901 cggcaagtac cgcagcctgt tcacctgcgt ggacgccgag acgatggcgg tgcgctggca
      961 ggtgctgatc gacggcaact gcgacctggt ggccacctcc tatgacggca agctggcggc
     1021 gaccaaccag tacaacacgg aaaacggcgc gcacttcgag gacatgatgt ccgccgagcg
     1081 cgatgcctgc gtattcttca acatcgctcg tatcgaggcg gcggtgcagg cgggcaagtt
     1141 caagacctac ggcgattcga aggtgccggt ggtcgacggc acgcaagcgg ccaacaagga
     1201 cccgaagacc gcgctgacgg cctacgtatc ggtgccgaag aacccgcacg gggtcaacgc
     1261 cagtcccgac cagaagtact tcatctgcgc gggcaagctc tcgcccacgg ccacggtgat
     1321 cgagctgtcg cgcgtgcttg gctggttcga cggcaagcag gagaagctcg acgatgccat
     1381 cgtcgccgag gtggagctgg gtctcgggcc gctgcacacg gccttcgatg gccgcggcaa
     1441 tgcctacacc acgctgttcc tcgacagcca gctcgtgaaa tggaatctcg atgcggcgat
     1501 caagttccac aagggtgaca agaacgccaa gtacgtggtc gatcggctgg acctgcagta
     1561 ccagcccggc cacgtcaacg cctcgcagtc cgagacggtt gcggccgacg gcaagtacct
     1621 ggcggtcggc tgcaagttct cgaaggaccg tttcctgccg gtcggaccgc tgcatccgga
     1681 gaacgagcag ctgatcgaca tctccggcca gaagatggtg ctgatggcgg accacccggt
     1741 gcgtggcgag ccgcatgact tcatcatctt taaacgcgag ctggtgcggc cgaagcaggt
     1801 ctacgcgctg gacgacttcc cgctggcgat caaggacccg aaggagtcgg gcgtgttccg
     1861 caatggccgc aaggtcaccg tgaagatcac gtcgcaggcg ccggccttca gcctgcgcga
     1921 gttcaagctg aagaagggtg acgaggtcac gctgatcctg accaatctcg acaagatcga
     1981 ggacctgacc cacggcttcg cgatcccgaa gtacaacgtc aacttcatcg tcaacccgca
     2041 ggagacggca tcggtcacct tcgttgccga caagccgggc gtgttctggt gctactgcac
     2101 ccacttctgc cacgcactcc acctggaaat gcgcacccgc atgattgtgg aggcctgagc
     2161 ccgagcagcc gcgcggccat ggtttcccct cgtggccgcg cgggacagac gagaacgaca
     2221 tgcagcccct gaagcatttt ggtggcatcg tggcgccctt ttcctgctgc tcatggccgg
     2281 ccgcgcccgg tccatgcggg cagtgcctac gacgcattgc cgccggacct gcgcaccgcc
     2341 cgcaaccttt gcgcactggt gccatgccag gaagtctttc caggcgcaga gagcttttcc
     2401 gagcgcatcg ggcagcctcc ctacgtcgcc aggtacggcc gc
//