From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Plasmid pTF-FC2 from Thiobacillus ferrooxidans mobA, mobB, mobC,
Date: 31 Dec 92 01:48:41 GMT
Distribution: bionet
Checksum: 31527 11

LOCUS       TFETFFC2     5317 bp ds-DNA             BCT       18-DEC-1992
DEFINITION  Plasmid pTF-FC2 from Thiobacillus ferrooxidans mobA, mobB, mobC,
            mobD, mobE genes, complete cds, ORF's 3, 5 and 8, complete cds, and
            merR gene, 3' end.
ACCESSION   M57717
KEYWORDS    MobA; MobB; MobC; MobD; MobE; merR regulatory protein.
SOURCE      Plasmid pTF-FC2 (from T.ferrooxidans) DNA.
  ORGANISM  Plasmid pTF-FC2
            Prokaryotae.
REFERENCE   1  (bases 1 to 5317)
  AUTHORS   Rohrer,J. and Rawlings,D.E.
  TITLE     Sequence analysis and characterization of the mobilization region
            of the broad-host-range plasmid, pTF-FC2, isolated from
            Thiobacillus ferrooxidans
  JOURNAL   J. Bacteriol. 174, 6230-6237 (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     CDS             complement(<1..1227)
                     /gene="mobA"
                     /note="ORF6"
                     /codon_start=1
                     /translation="MIALSQEAVRSKDTINHYVLSWREGEQPSPEQVEEAVSIFMDEL
                     GVKDHQAIYGLHADTDNLHLHLAINRVHPETLKVVKINNGFDIEAAHKAIARIENAQG
                     WQREQNGRYQVLENGELGREHIDKDKPRQPAQPKRDMENRTGEKSAERIAIEDGAPII
                     KKAQTWEQLHRELAAKGMRYEKTGSGATLFVGDVGVKASSADRDASLSKLQKRLGAYQ
                     PPQRQQVAQREPEPIKPDVPGWKDYITGRKAHYSEKNADKLVQDKRQEQERKQLAEQQ
                     KARRDELMRGNWKGKGEVLNAMRSVIAAEQAAEKAALKEKHQKQREQHRQQFRPYPDL
                     EQWQRMQKSPELAEQWRHRASEPQRIEGDRSEPPTPRDIRAYQPEIVGQQVHYSRKEE
                     GGRGGGVSFVDKGKSID"
     CDS             2007..2363
                     /gene="mobC"
                     /note="ORF1"
                     /codon_start=1
                     /translation="MKYTTEQLEAIASKLRDMPQVEKKKQEHSKQEAVRVLSKEIAAL
                     QKRGYTLDQISETLRGEGLSIATPTLKSYLQRAKPTKKAPVQAPGDTPPPAPAVKKPA
                     DTSKATFTPKPDSDDI"
     CDS             2376..3059
                     /gene="mobD"
                     /note="ORF2"
                     /codon_start=1
                     /translation="MAKSIYLIGGSKGGVGKSLVTMATVDYLQERGESVLLIESDTSN
                     PDVWKAYKESTETELINLDEADGWIQLVNLCDSKPDSVVVINAAARNNKGVSAYGETL
                     NSTLAELKRKLVTLWVINRQRDSLELLKEYMDAIPNADVHVVRNGHFGEEKKFELYNG
                     SKLRTAVEERGGQSVTFPDMADRVSDDIYSKRMSISVALKELPIGNRAELTRWRNEAK
                     KVLEGVIHE"
     CDS             3052..3693
                     /gene="mobE"
                     /note="ORF4"
                     /codon_start=1
                     /translation="MSDLDDSFAKLLGRQPSDAERQSLYRVRDALGLKNNDALWLVLM
                     ALQHYQGQYEKFPQAIAQAAKDTLVNFKVTADATVKASAEAAKADLAQAVAAAAQEVA
                     HNTSAKQMWQWAAGCIAVAFLCVGLFGWYMHSSGKDSGYQAGYGAGYTEAKDEKAAAA
                     WANTPEGRTAYRFAQSGELQRLARCSGKGWKVEKGACYPYPVANEGTYGWALP"
     CDS             3678..4190
                     /note="ORF3"
                     /codon_start=1
                     /translation="MGVAMIDALNIATMPIADKVHRHMLASYYALQLDALQAEAKRLG
                     YFFAQADTAATMPPVLADCLAWAVEYRRRCYLYPNCPESWERHTADSMSDGYAQEHCR
                     SMLAALAALGIRLQEGQQAYALLAAEECRQREKASLPPEPSPLSEVEVSSFVDEFFTK
                     LDAPKEEVPT"
     CDS             4187..4354
                     /note="ORF5"
                     /codon_start=1
                     /translation="MSTTAKIDFETGLFDEGQYEEAPDLPAIQPKRRTSRAPFIVAGG
                     LLLGVVSENGN"
     CDS             4585..4842
                     /note="ORF8"
                     /codon_start=1
                     /translation="MTTKRKVEVFSAGCPSCQTAIELVNRLACGSCEVSILDMNDINV
                     AKRARDLGVRSVPAVAINGQLASCCSGSGIEEQALRWPRQA"
     CDS             4951..5317
                     /partial
                     /product="merR regulatory protein"
                     /codon_start=1
                     /translation="MEDTMHTIGKVATLAGVSTDTLRYYEKEHLIEPASRTAAGYRLY
                     NDEAVRRIRFIKTAQHCGFSLSDIQELLTLKRTDSACCEDVRSVAIEKRLRIARKLRV
                     LQAMSAALNDLIQSCEGGAA"
BASE COUNT     1118 a   1569 c   1553 g   1077 t
ORIGIN      
        1 atcgatgctt ttgcccttgt ccacgaaaga cacacccccg ccccgccccc cttcttcctt
       61 ccggctgtaa tgcacctgct ggcccacgat ttcaggctga taggcgcgaa tgtcgcgcgg
      121 cgtcggcggc tcgctgcggt cgccctcgat gcgctgcggc tcgctggccc ggtgtcgcca
      181 ctgctcggca agctccgggc ttttctgcat ccgctgccac tgctccaggt cgggataggg
      241 tctgaactgc tgccggtgct gctcgcgctg cttctggtgc ttctccttca aggcggcttt
      301 ctctgcggcc tgctcggcgg caatcacgct gcgcatggcg ttgagcactt cgcccttgcc
      361 tttccagttg ccgcgcatga gttcatcgcg gcgggccttc tgctgctcgg caagttgctt
      421 gcgctcctgc tcctgccgct tgtcctgtac cagcttgtcc gcgttcttct ccgagtagtg
      481 cgccttgcgc ccggtgatgt agtccttcca tcccggcacg tctggcttga tcggctccgg
      541 ctcgcgctgt gctacctgct gacgctgcgg cggctggtaa gctccgagtc gcttttgcaa
      601 cttggatagg ctcgcgtccc ggtctgcgct gctggccttc acgccaacat caccgacaaa
      661 gagcgtcgcc ccgctgcctg ttttctcgta gcgcatcccc ttggctgcta actcgcggtg
      721 caactgctcc cacgtctgcg cctttttgat gatcggtgcg ccgtcctcaa tggcgatgcg
      781 ttcggcggat ttctccccgg tgcggttttc catgtcgcgc ttcggctggg ctggctggcg
      841 cggcttgtcc ttgtcgatgt gctcccggcc taactcacca ttctccaaca cctgataacg
      901 cccgttctgc tcacgctgcc agccttgtgc gttctcgatt cgtgctatcg ccttgtgcgc
      961 tgcctcaatg tcgaagccgt tattgatctt caccaccttg agcgtttcag gatgcacgcg
     1021 gttgatggca aggtgcaggt gcaagttgtc cgtgtcggcg tgcaaaccat agatggcttg
     1081 atggtcttta acacctagct catccatgaa aatgcttacc gcttcttcga cctgctccgg
     1141 gctgggctgc tcgccttctc gccaactcag gacatagtga ttgatcgtat ccttgcttcg
     1201 cactgcctct tgtgacaagg caatcatttc tgccgtctgg ctctgtggtt catcggtgat
     1261 gaatccacgc gccccggcat ggatgcactt ctcctgcgag ttctcacgct caggctcacg
     1321 gatgtagcca gttaggcggg aaacgcgaac cgccttgctt gctgccttct gcgggttctt
     1381 aaccttcttg acgatcacgg ctcagtttcc cgatataggc tttcaagacc acaagcgccc
     1441 cggctgtgtc cttgctgtac acgcctccgc tctcgttgtg gatgtgctta agcaatccac
     1501 cgatgcggcg caactctttg agcattaccg cgtcggcgct ggcaatgatc ggcctgccaa
     1561 agtagcggcg gcggacaagc tccgacatgc tcaacccggc aaggtcggca tcctctttca
     1621 agcgggcctt ctccgctgcg gtcagccgta cattgataac ggcgtccagc ggttccggcc
     1681 cctgcgtttc aaatggcatc gtgtctcctt ctcgcggggg gcgggcgggg tgtgtcttgg
     1741 acagtaagaa gcgagagcgc cgcactgtcc ggggtcaagg ggatacccct tgcaggataa
     1801 gccagaccgt gagaagcgaa gcgccgaatg ggcaggcgtc cgttggaacc acgtggcgaa
     1861 gccatgtgtg ttacaacggt catcctgtat tgctcaaccg ctctactatc atatcagcag
     1921 tttaggcaca ctagatgcac tttgcaacaa gcataaaagg cgctatgctg tcaactccga
     1981 aaccagtaag caaaggagtt agcagaatga aatacacgac agagcagcta gaggctatcg
     2041 catcgaagct gcgtgatatg ccacaagtcg aaaaaaagaa gcaggagcac agcaagcaag
     2101 aggccgtgcg cgtgctatca aaagagatcg cggctttgca aaagcgcgga tacacgcttg
     2161 accagatttc ggaaacgctt cgcggtgaag gtctgagcat cgcaaccccg acactcaaga
     2221 gctatttgca gagagcaaag ccaacgaaaa aagccccggt gcaagcgccg ggagacacac
     2281 ccccgcccgc ccccgccgtc aagaagcctg cggatacatc gaaggcgaca tttaccccaa
     2341 aacccgacag cgacgacatt taaggagtaa gcaacatggc aaaaagcatt tacctgatcg
     2401 gcggcagcaa gggcggcgtt ggcaagtcgc ttgtcacgat ggcaacggtt gattacctgc
     2461 aagaacgcgg cgaaagcgta ttgctgattg aatccgacac ctctaacccg gacgtgtgga
     2521 aggcatacaa ggaaagcacg gaaacggagc ttatcaatct ggatgaggcg gacggatgga
     2581 ttcagctagt gaacttgtgc gatagcaagc cggatagcgt cgtggtaatt aacgcggctg
     2641 cgaggaataa caagggagtg agcgcctacg gtgaaacatt gaatagcaca cttgcggaac
     2701 tcaagcgcaa gctggtcacg ctgtgggtga ttaaccgcca acgggatagc ctcgaactgc
     2761 tcaaggaata catggatgca atcccgaacg cggatgttca tgtggtgcgc aatggacact
     2821 tcggagagga aaagaaattc gagctttaca acggctcaaa gctgcgcacg gcagtagagg
     2881 aacgcggcgg gcagtcggtg acattcccgg atatggccga ccgggtgagc gatgacattt
     2941 acagcaagcg catgagcatt tcggtagcac tcaaggaact gccaattggc aaccgtgcgg
     3001 aacttacccg gtggcgcaat gaggctaaaa aggtattgga gggcgtaatc catgagtgac
     3061 cttgacgaca gctttgcaaa gctgctcggc aggcaaccca gtgacgcgga gcggcaaagc
     3121 ctttaccgtg tgcgggatgc actcgggctg aaaaacaatg atgcgctctg gctggtgctc
     3181 atggccttgc agcactacca aggccagtat gagaagttcc cgcaagcgat agcacaagca
     3241 gccaaagata ccttggtcaa tttcaaggta acggcggatg caaccgtgaa agcatcagcg
     3301 gaagcagcca aggcggattt agcgcaggcg gtagcagcag cagcgcaaga ggtcgcgcac
     3361 aacacatctg caaagcagat gtggcagtgg gcggcgggct gcatcgccgt ggccttcctg
     3421 tgcgtcggcc tgttcggctg gtacatgcac agcagcggca aggattccgg ctaccaagcc
     3481 ggatacggcg cgggctacac ggaagccaag gacgagaaag cggcggcggc atgggcaaac
     3541 acgccagaag gtcgaacggc ctaccgcttc gcccagtccg gcgaactgca acgccttgcc
     3601 cggtgcagtg gcaagggctg gaaggtcgag aaaggcgctt gctaccccta cccagtagcg
     3661 aatgagggca cttacggatg ggcgttgcca tgatcgacgc gctcaacatc gccacgatgc
     3721 cgattgcaga caaggtgcat cggcacatgc tggcaagcta ctacgccttg caactggatg
     3781 ccttgcaggc cgaagccaag cggctcggct acttcttcgc gcaagccgac acggcggcaa
     3841 ccatgccgcc agtgctggcc gattgcttgg cgtgggcggt cgagtaccgg cgcaggtgct
     3901 acctgtaccc gaattgcccg gaaagctggg aacgccacac cgccgacagc atgagcgacg
     3961 gttacgccca agagcattgc cgctcgatgc tcgcggcgct ggccgcgctc ggcatccgtt
     4021 tgcaggaagg ccagcaagcc tacgccctgc tggcggcgga ggaatgccgc caacgcgaga
     4081 aagccagctt gccacccgag ccttcgccac tctcggaagt cgaagtgtcc agcttcgtcg
     4141 atgagttttt caccaagctg gacgcaccca aggaggaagt cccgacatga gcacaaccgc
     4201 caagatcgac tttgaaaccg gcctgttcga tgagggccag tacgaggaag cgccagacct
     4261 gccagccatc cagccgaaac ggcggacaag tcgcgccccg ttcatcgtgg ccggtggctt
     4321 gctgcttggg gtcgtctcag aaaacggaaa ttaaagcacg ctaaggcacg acaagcgtgc
     4381 gcatatgctg cttcacggac tgaaggggct gttttcctgt tcccggtgcg ggcgatccgg
     4441 gcgttgcgcg aaaccgcata acccgctcag ttagtacttg acgttgaagt aagggccaag
     4501 gtctatcgta atgacgcgcg gcgctcatgg cttctccggc cgggagttct cgcgcacctg
     4561 accacacttt tttggagaaa taccatgact accaaacgca aagtcgaagt tttcagcgcg
     4621 ggatgcccct cctgccagac cgccattgaa ctcgtcaatc gcctcgcatg cggttcgtgc
     4681 gaggtctcaa tcctcgatat gaacgacatc aatgtggcca aacgcgcccg tgatctcggt
     4741 gttcggtcgg tgccggcggt cgccatcaac ggccagctgg cttcgtgctg ctccggcagc
     4801 ggcatcgagg aacaggcgct tcgctggcct cggcaagcct aggcctcccc atcggcacgc
     4861 gcgtgcctct cgctggaagc tcgcgattaa tttcttaggc ggaactcttg accatggtgt
     4921 tcactccaaa gtttatatta acacttacaa atggaggaca ccatgcacac catcggcaag
     4981 gtggcaacgc tagccggagt cagcaccgat acgctgcgct actatgaaaa agagcacctg
     5041 atcgaacctg catctagaac cgctgcgggc taccggctgt acaacgatga agcggtgcgg
     5101 cgcatccgtt ttatcaaaac cgctcagcac tgcggattct cgttgtcgga cattcaggag
     5161 ttgctgacgc tgaagcgaac tgatagtgcc tgttgcgagg atgtgcgcag cgtggccatc
     5221 gagaagaggc tgcgcatcgc gcgcaaacta cgggtcttgc aggccatgtc ggccgccctc
     5281 aatgacttga tccagagttg cgaaggcggt gctgcag
//