From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: E.coli malS gene for 1,4-alpha-D-glucan glucanohydrolase
Date: 10 Apr 92 10:04:40 GMT
Distribution: bionet
Checksum: 22454     9

LOCUS       ECOMALS      3617 bp ds-DNA             BCT       10-APR-1992
DEFINITION  E.coli malS gene for 1,4-alpha-D-glucan glucanohydrolase
ACCESSION   X58994
KEYWORDS    1,4-alpha-D-glucan glucanohydrolase; malS gene.
SOURCE      Escherichia coli DNA.
  ORGANISM  Escherichia coli
            Prokaryota; Bacteria; Gram-negative facultatively anaerobic rods;
            Enterobacteriaceae.
REFERENCE   1  (bases 2714 to 3617)
  AUTHORS   Liu,L., Whalen,W., Das,A. and Berg,C.M.
  TITLE     Rapid sequencing of cloned DNA using a transposon for bidirectional
            priming: sequence of the Escherichia coli K-12 avtA gene
  JOURNAL   Nucleic Acids Res. 15, 9461-9469 (1987)
  STANDARD  full automatic
REFERENCE   2  (bases 1 to 3617)
  AUTHORS   Schneider,E.
  JOURNAL   Unpublished (1991)
  STANDARD  full automatic
REFERENCE   3  (bases 1 to 3617)
  AUTHORS   Schneider,E., Freundlieb,S. and Boos,W.
  TITLE     Molecular characterisation of the periplasmic alpha-amylase of..
  JOURNAL   J. Biol. Chem. 267, 5148-5154 (1992)
  STANDARD  full automatic
COMMENT     *source: strain=K12;
            for overlapping sequences see: Y00490
            From EMBL    entry ECMALS;  dated 24-MAR-1992.
FEATURES             Location/Qualifiers
     CDS             1..478
                     /pseudo
                     /note="divergent ORF"
     protein_bind    529..549
                     /bound_moiety="cAMP /CAP"
     misc_binding    692..701
                     /bound_moiety="MalT"
     misc_binding    705..714
                     /bound_moiety="MalT"
     misc_binding    729..738
                     /bound_moiety="MalT"
     -10_signal      759..764
     mRNA            771..>2828
                     /evidence=EXPERIMENTAL
     prim_transcript 771..>2828
                     /evidence=EXPERIMENTAL
     CDS             798..2828
                     /EC_number="3.2.1.1"
                     /product="alpha-amylaseprecursor"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGEYTLQIDTRSGTPT
                     LMISIQNAAEPVASLVRECPKWDGLPLTVDVSATFPEGAAVRDYYSQQIAIVKNGQIM
                     LQPAATSNGLLLLERAETDTSAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGNEADLRTLVDSAHQRGIRILFDVVMNHTGYATLADMQEYQFGALYL
                     SGDEVKKSLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKMDTHAKAIDGYTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASAALREWKKANPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCLAQMDTTWQQMAEKLQGFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ASVAHWQKISQFRARHPAIGAGKQTTLLLKQGYGFVREHGDDKVLVVWAGQQ"
     sig_peptide     798..848
                     /note="alpha-amylase"
     mat_peptide     849..2825
                     /EC_number="3.2.1.1"
                     /product="alpha-amylase"
     conflict        2717..2718
                     /note="replace(2717..2718,'at')"
                     /citation=[1]
                     /partial
     conflict        2727..2729
                     /note="replace(2727..2729,'')"
                     /citation=[1]
                     /partial
     conflict        2791^2792
                     /note="replace(2792^2791,'g')"
                     /citation=[1]
                     /partial
     stem_loop       2831..2871
     stem_loop       2897..2920
     conflict        2899^2900
                     /note="replace(2900^2899,'tg')"
                     /citation=[1]
                     /partial
     conflict        2902
                     /note="replace(2902,'g')"
                     /citation=[1]
                     /partial
     conflict        2916
                     /note="replace(2916,'')"
                     /citation=[1]
                     /partial
     conflict        3002^3003
                     /note="replace(3003^3002,'g')"
                     /citation=[1]
                     /partial
     conflict        3512^3513
                     /note="replace(3513^3512,'g')"
                     /citation=[1]
                     /partial
BASE COUNT      902 a    926 c    920 g    869 t
ORIGIN
        1 ctgcagccat cgtcgccacc atactggtgg ggataatgtc tacgcgttca agcaaggtat
       61 tccacgggat ttttcgcgta ttaccggacc acttcacctt gtagcgtttg gcgatgtctt
      121 tcagacgcgc acgctcagca ggtgaccatt ggccctgata ctgttttgaa atgagccagt
      181 tacgttccgc agtaatggcc gcattttggc tggtaatgta aggcattacg gtccggagaa
      241 acgccttttt ccttggtgtt ccggaagggt attttcgcaa atcaggaagt gaactactct
      301 ttgcactatt gcgagaatac tcttgtttac tgcttacctg tttattacta gctttagtta
      361 agtgggactt ttgactcgct gttgttgtgt gcgtcttcgc taacacctca ctcgaaaaca
      421 ccagagtgag taacataaga atcatcgccc catatcgtcg tatgggagtc aaaatcatca
      481 ggtctcctgg tcggatttaa tcattccaac accttatatt tttcacaaat ttgagagttg
      541 aatctcaaat catatcaaaa atagctgtca agagcacccc aaggaatagt ccaaatctga
      601 aactatgtca cgtgttaacg attcagattg gcgctaaatc gcagaaaatg tgggggttat
      661 cgcaaaattc agccgttttt tgcgcgagat cgctcaccct tgcttctcat cctgtggact
      721 taccgctcag ggatgagttt tgtttggctt atcgctggca aactgtctga aatcgcagca
      781 ataaggactc atccgccatg aaactcgccg cctgttttct gacactcctt cctggcttcg
      841 ccgttgccgc cagctggact tctccggggt ttcccgcctt tagcgaacag gggacaggaa
      901 catttgtcag ccacgcgcag ttgcccaaag gtacgcgtcc actaacgcta aattttgacc
      961 aacagtgctg gcagcctgcg gatgcgataa aactcaatca gatgctttcc ctgcaacctt
     1021 gtagcaacac gccgcctcaa tggcgattgt tcagggacgg cgaatatacg ctgcaaatag
     1081 acacccgctc cggtacgcca acattgatga tttccatcca gaacgccgcc gaaccggtag
     1141 caagcctggt ccgtgaatgc ccgaaatggg atggattacc gctcacagtg gatgtcagcg
     1201 ccactttccc ggaaggagcc gccgtacggg attattacag ccagcaaatt gcgatagtga
     1261 agaacggtca aataatgtta caacccgctg ccaccagcaa cggtttactc ctgctggaac
     1321 gggcagaaac tgacacatcc gcccctttcg actggcataa cgccacggtt tactttgtgc
     1381 tgacagatcg tttcgaaaac ggcgatccca gtaatgacca gagttacgga cgtcataaag
     1441 acggtatggc ggaaattggc acttttcacg gcggcgattt acgcggcctg accaacaaac
     1501 tggattacct ccagcagttg ggcgttaatg ctttatggat aagcgcccca tttgagcaaa
     1561 ttcacggctg ggtcggcggc ggtacaaaag gcgatttccc gcattatgcc taccacggtt
     1621 attacacaca ggactggacg aatcttgatg ccaatatggg caacgaagcc gatctacgga
     1681 cgctggttga tagcgcacat cagcgcggta ttcgtattct ctttgatgtc gtgatgaacc
     1741 acaccggcta tgccacgctg gcggatatgc aggagtatca gtttggcgcg ttatatcttt
     1801 ctggtgacga agtgaaaaaa tcgctgggtg aacgctggag cgactggaaa cctgccgccg
     1861 ggcaaacctg gcatagcttt aacgattaca ttaatttcag cgacaaaaca ggctgggata
     1921 aatggtgggg aaaaaactgg atcagaacgg atatcggcga ttacgacaat cctggattcg
     1981 acgatctcac tatgtcgcta gcctttttgc cggatatcaa aaccgaatca actaccgctt
     2041 ctggtctgcc ggtgttctat aaaaacaaaa tggataccca cgccaaagcc attgacggct
     2101 atacgccgcg cgattactta acccactggt taagtcagtg ggtccgcgac tatgggattg
     2161 atggttttcg ggtcgatacc gccaaacatg ttgagttgcc cgcctggcag caactgaaaa
     2221 ccgaagccag cgccgcgctt cgcgaatgga aaaaagctaa ccccgacaaa gcattagatg
     2281 acaaaccttt ctggatgacc ggtgaagcct ggggccacgg cgtgatgcaa agtgactact
     2341 atcgccacgg cttcgatgcg atgatcaatt tcgattatca ggagcaggcg gcgaaagcag
     2401 tcgactgtct ggcgcagatg gatacgacct ggcagcaaat ggcggagaaa ttgcagggtt
     2461 tcaacgtgtt gagctacctc tcgtcgcatg atacccgcct gttccgtgaa gggggcgaca
     2521 aagcagcaga gttattacta ttagcgccag gcgcggtaca aatcttttat ggtgatgaat
     2581 cctcgcgtcc gttcggtcct acaggttctg atccgctgca aggtacacgt tcggatatga
     2641 actggcagga tgttagcggt aaatctgccg ccagcgtcgc gcactggcag aaaatcagcc
     2701 agttccgcgc ccgccatccc gcaattggcg cgggcaaaca aacgacactt ttgctgaagc
     2761 agggctacgg ctttgttcgt gagcatggcg acgataaagt gctggtcgtc tgggcagggc
     2821 aacagtaact tttccggctt cccgttcgtc agtacctcgg gaagccgcca accaggataa
     2881 aatgtcagcc ctaatcagcg ttgcaggata aagcaccgct cactcttcaa cagaccgatt
     2941 tgcaccccag caaatgtagc gttattgtta ccttccttgc tacagagttc gacagatatc
     3001 ccgctatgac attctccctt tttggtgaca aatttacccg ccactccggc attacgctgt
     3061 tgatggaaga tctgaacgac ggtttacgca cgcctggcgc gattatgctc ggcggcggta
     3121 atccggcgca gatcccggaa atgcaggact acttccagac gctactgacc gacatgctgg
     3181 aaagtggcaa agcgactgat gcactgtgta actacgacgg tccacagggg aaaacggagc
     3241 tactcacact gcttgccgga atgctgcgcg agaagttggg ttgggatatc gaaccacaga
     3301 atattgcact aacaaacggc agccagagcg cgtttttcta cttatttaac ctgtttgccg
     3361 gacgccgtgc cgatggtcgg gtcaaaaaag tgctgttccc gcttgcaccg gaatacattg
     3421 gctatgctga cgccggactg gaagaagatc tgtttgtctc tgcgcgtccg aatattgaac
     3481 tgctgccgga aggccagttt aaataccacg tcgattttga gcatctgcat attggcgaag
     3541 aaaccgggat gatttgcgtc tcccggccga cgaatccaac aggcaatgtg attactgacg
     3601 aagagttgct gaagctt
//