HDTenEightyP/NetNews huggingface dataset:
From: todd@behemoth.genetics.wisc.edu (Todd Richmond)
Subject: Most current genetic map?
Organization: UW Genetix
Distribution: bionet
Date: Fri, 4 Sep 1992 23:00:09 GMT


I'm looking for the most current genetic map
of Arabidopsis.  I'm busy mapping some cDNA's
with some RI lines and would like to see if
there's anything close to my clones.  Using
the new Arabidopsis database would be great-but
the only way for me to access it right now is
text only.  Are there any IBM or Mac programs
with accompanying data files which will allow
me to graphically display the most current map?

Email or post-if there's enough response I'll
summarize and post the results.

Thanks, 

Todd

--
*********************************************************
Todd Richmond  UW-Madison Dept of Genetics
todd@genetics.wisc.edu         
**insert standard disclaimer**
From: BTNYDWM@MVS.UCC.OKSTATE.EDU
Newsgroups: bionet.genome.arabidopsis
Subject: Just a Test
Date: 9 Sep 92 16:21:00 GMT
Distribution: bionet

This is just a test.
Please ignore.
From: CHU@MIS.BARNARD.COLUMBIA.EDU
Newsgroups: bionet.genome.arabidopsis
Subject: cDNA library kits
Date: 11 Sep 92 17:47:03 GMT
Distribution: bionet

I'm collecting various tissues from arabidopsis (e.g. pedicel and internodal
tissue) for organ and tissue specific cDNA libraries.  Can anyone give me 
any advice as to the "best" or most useful kit to purchase (in vitrogen,
stratagene, promega etc)?

Thanks for your time.

Nate Chu
Chu@mis.Barnard.Columbia.edu
From: SLS6Q@CC.USU.EDU
Newsgroups: bionet.genome.arabidopsis
Subject: terminate subscription
Date: 14 Sep 92 16:52:56 GMT
Distribution: bionet

I am leaving my current address and wish to discontinue my subscription
to the arabidopsis network. I will resubcribe when I get my new email address.
Thanks.
From: BTNYDWM@MVS.UCC.OKSTATE.EDU
Newsgroups: bionet.genome.arabidopsis
Subject: Mapping survey
Date: 9 Sep 92 14:11:00 GMT
Distribution: bionet

To the Arabidopsis Community:

     Please note that an important mapping survey will soon be
distributed in two different ways to the Arabidopsis community:
(1) through this electronic network; and (2) by postal mail to
individuals selected from the mailing list for the Arabidopsis
Biological Resource Center at Ohio State.  The purpose of this
survey is to update linkage information for incorporation into
existing databases.  Some of you may prefer to respond through
email.  Others may find it easier to complete the forms sent
by postal mail.  In either case, please respond without delay.
Results will be used to update linkage information for visible
and molecular markers.  A list of gene symbols currently used
in your laboratory is also requested.  This list should include
only mutants examined in some detail.  All information obtained
will be incorporated into AAtDB (Arabidopsis thaliana database
established by Mike Cherry, Sam Cartinhour, and Howard Goodman)
and AIMS (Arabidopsis Information Management System database
being developed by Sakti Pramanik and Randy Scholl).  I have
organized this survey with assistance from Randy Scholl, Maarten
Koornneef, Elliot Meyerowitz, Joe Ecker, Chris Somerville, and
Mike Cherry.  We look forward to receiving your response.
Regards - David Meinke
From: bafp3@central.sussex.ac.uk (Neil Butt)
Newsgroups: bionet.genome.arabidopsis
Subject: seed promoters
Date: 7 Sep 92 15:14:21 GMT
Distribution: bionet
Original-To: arab-gen@uk.ac.daresbury
X-Mailer: ELM [version 2.3 PL11]

Dear networkers,
I am interested in finding anything out about seed proteins/transcripts. I 
am especially interested in genes which may be tanscribed and stored in the 
seed before the poduct is translated. One such protein is Carboxypeptidase C, 
however, no sequence for this appears to be in the databases. If anyone has 
this or other sequences that may confer such expression I would be extremely 
interested to hear from you.
Thanks in advance
Neil Butt
Biological Sciences
University of Sussex
Brighton BN1 9QG
U.K
E-mail U.K       bafp3@uk.ac.sussex.central
       Abroad    bafp3@central.sussex.ac.uk
From: scolnipa@esvax.dnet.dupont.com
Newsgroups: bionet.genome.arabidopsis
Subject: Position
Date: 15 Sep 92 21:34:36 GMT
Distribution: bionet



The Du Pont Company has an immediate job opening for a scientist 
with a Masters degree in biology to work on Arabidopsis molecular 
genetics.  The succesful candidate will join  a team of plant 
physiologists, biochemists, and geneticists to ascertain the 
resistance potential of plants to new herbicides and the possible 
mode of action of these chemicals.   Initially, activities will involve 
mutagenesis and screening of Arabidopsis plants for herbicide 
resistance.  Similar activities with microbes and other plant 
species could be envisioned in the future.

Du Pont is located in Wilmington, Delaware, an attractive east Coast 
location near Philadelphia and Baltimore.
We require:

%	Ability to organize, schedule, prioritize and carry out multiple 
tasks of a technical nature in an effective and timely manner.
%	Familiarity with (or ability to rapidly learn) modern laboratory 
techniques and instrumentation.
%	Good interpersonal skills and willingness to be part of a multi-
disciplinary research effort .
%	Good written and oral communications skills.
%	An interest in developing a strong research program in novel 
approaches to  agrichemical discovery.
%	Good computer skills.

Please send c.v. and names of three references by fax (302-695-
8480) or e-mail
(scolnipa@esvax.dnet.dupont.com).
From: samodena@csemail.cropsci.ncsu.edu (S. A. Modena)
Newsgroups: bionet.genome.arabidopsis
Subject: Re: non-radioactive probes for Arabidopsis?
Date: 12 Sep 92 04:19:50 GMT
Distribution: bionet
Organization: Crop Science Dept., NCSU, Raleigh, NC 27695-7620

In article <9209101215.AA04941@dogwood.botany.uga.edu> russell@DOGWOOD.BOTANY.UGA.EDU (Russell Malmberg) writes:
>
>
>Has anyone tried using the non-radioactive systems to probe
>Arabidopsis southerns and northerns?
>
>I'm thinking about trying out the digoxigenin labelled
>DNA, followed by anti-digoxigenin-alkaline phosphatase,
>and then either chemiluminescence or standard blue BCIP/NTB
>detection mechanisms. 
>
>Has anyone had success with this with Arabidopsis or other
>plant blots?  If I obtain several replies I will summarize and
>re-post to this group.
>
>Russell Malmberg
>russell@dogwood.botany.uga.edu
>------------------------------
>
>

I would suggest contacting:  Dave Hoisington
                             Molecular Genetics Laboratory
			     CIMMYT
			     Lisboa 27
                             Apdo. Postal 6-641
                             06600 Mexico, D.F.
                             MEXICO

         tel. 011-525-954-2100    FAX:  011-525-954-1069
         (those numbers assume that you are dialing from the U.S.A.) 

A year ago, mail correspondance took 3 to 6 weeks for an Air Mail letter
to reach the P.O Box in Mexico City....I'm not sure if things have
improved.  Dave's EMail address currently bounces so I didn't give it.
The telephone number is directly for CIMMYT in El Batan and the CIMMYT 
operators can respond either in Spanish or English.

For a "preview" of what their non-radioisotopic methods can do (in maize)
you might contact:    Michel Ragot
		      Dept. of Genetics
                      Box 7614
		      North Carolina State University
		      Raleigh, NC  27695-7614

       tel. 1-919-515-2289   FAX: 1-919-515-3355
       NCORN1@NCSUMVS.BITNET

Michel was in the CIMMYT molecular genetics lab last year doing that
stuff and so, I believe, can be specific on details and performance.

The CIMMYT lab is specifically geared to developing molbio genetic
techniques for maize and wheat improvement that can be successfully
transfered to labs working under the constraints commonly found
once one leaves the USA/Western Europe/Japan/Australia.

Hope this helps.

Steve
---
+------------------------------------------------------------------+
|     In person:  Steve Modena     AB4EL                           |
|     On phone:   (919) 515-5328                                   |
|     At e-mail:  nmodena@unity.ncsu.edu                           | 
|                 samodena@csemail.cropsci.ncsu.edu                |
|                 [ either email address is read each day ]        |
|     By snail:   Crop Sci Dept, Box 7620, NCSU, Raleigh, NC 27695 |
+------------------------------------------------------------------+
         Lighten UP!  It's just a computer doing that to you.
OOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOO
From: BIOCUKM@MVS.UCC.OKSTATE.EDU
Newsgroups: bionet.genome.arabidopsis
Subject: How much leaf for PCR?
Date: 6 Sep 92 21:57:00 GMT
Distribution: bionet

Can you recommend:
How many mg of Arabidopsis leaf tissue to use in preparation
of DNA for 30 cycle PCR amplification of single copy sequences?
Thanks,
Ulrich Melcher
From: bjkang@RKNA50.RIKEN.GO.JP (Bong-Joong Kang)
Newsgroups: bionet.genome.arabidopsis
Subject: Revive SEEDS
Date: 7 Sep 92 02:14:18 GMT
Distribution: bionet
From: ANMR1@UORDB2.bitnet
Newsgroups: bionet.genome.arabidopsis
Subject: RE: How much leaf for PCR
Date: 8 Sep 92 13:12:00 GMT
Distribution: bionet

We can routinly amplify by PCR DNA isolated from approximately 50-200
leaf cells (microscopic sectors). We first make protoplasts fom these
cells in mannitol using cellulase and pectinase. The protoplasts are
spun down and resuspended in the following buffer, heated to 55degreeC
for 1 hour, boiled for 5 mins, spun again and the supernatant (typically)
20-30 microliters) is kept frozen until PCR. For each PCR we use 5-10
microliters of that DNA.
Buffer:
50 mM KCl
        10 mM Tris.HCl, pH 8.3
        2.5 mM MgCl2
        0.45% NP-40
        0.45% Tween-20
        3microl/10 ml of Proteinase K (20 mg/ml stock, kept frozen at -20degreeC
) to be
added just before the experiment. (filter sterilized)

Let me know if you have any problem.
Animesh Ray
From: russell@DOGWOOD.BOTANY.UGA.EDU (Russell Malmberg)
Newsgroups: bionet.genome.arabidopsis
Subject: non-radioactive probes for Arabidopsis?
Date: 10 Sep 92 12:15:52 GMT
Distribution: bionet



Has anyone tried using the non-radioactive systems to probe
Arabidopsis southerns and northerns?

I'm thinking about trying out the digoxigenin labelled
DNA, followed by anti-digoxigenin-alkaline phosphatase,
and then either chemiluminescence or standard blue BCIP/NTB
detection mechanisms. 

Has anyone had success with this with Arabidopsis or other
plant blots?  If I obtain several replies I will summarize and
re-post to this group.

Russell Malmberg
russell@dogwood.botany.uga.edu
------------------------------
From: 21847LAB@MSU.EDU ("Arabidopsis")
Newsgroups: bionet.genome.arabidopsis
Subject: angustofolia Landsberg wanted
Date: 7 Sep 92 14:44:00 GMT
Distribution: bionet

I would very much like to obtain an angustofolia strain of Landsberg erecta
which does not contain any other extraneous mutations.  Please let me know if
you have such a line available.  Thanks a lot.
                             Sincerely,
                             Ruth Wilson
                             DOE Plant Research Lab.
                             Michigan State University
                             East Lansing, MI  48824
                             ph.(517)-355-5197
From: bjkang@RKNA50.RIKEN.GO.JP (Bong-Joong Kang)
Newsgroups: bionet.genome.arabidopsis
Subject: Revive SEEDS
Date: 7 Sep 92 02:26:54 GMT
Distribution: bionet

Dear Networkers,
I have some strange problem with non-germinating Arabidopsis seeds
harvested five months ago.  These seeds doesn't germinate under 
general condition with cold treatment and/or GA supply.
What can I do to revive these seeds?  Hope someone can help.

Bong J. Kang
FRP, RIKEN, JAPAN
FAX : 81-48-462-4691
E-mail : bjkang@rkna50.riken.go.jp
From: ZIMMERE@SIMSC.BITNET
Newsgroups: bionet.genome.arabidopsis
Subject: codon usage in nuclear relative to chloroplast genes
Date: 11 Sep 92 23:14:00 GMT
Distribution: bionet

Dear Netters:

I am trying to get some info for a colleague:

Is there anything published cf. codon usage in plant nuclear genes relative
to plant chloroplast genes.  Specifically, my colleague Bruce Kirchoff is
interested in codon usage in monocots, particularly plants related to the
Zingiberales .  We have chloroplast gene sequence for rbcL for that group,
so we are particularly interested in whether the rbcL codon usage is
similar /predictive for nuclear genes of same organism?  Will usage
be a function of tissue/developmental stage?  If so, he is interested
in floral/inflorescence genes?

Thanks for the info.  I will try to post summary of responses.

--Liz Zimmer, Smithsonian Lab of Molecular Systematics
"Zimmere@simsc.bitnet"
From: vance_baird@QUICKMAIL.CLEMSON.EDU ("Vance Baird")
Newsgroups: bionet.genome.arabidopsis
Subject: Pollen germination/growth
Date: 11 Sep 92 13:50:43 GMT
Distribution: bionet


                       Subject:                               Time:9:47 PM
  OFFICE MEMO          Pollen germination/growth              Date:9/11/92
Does anyone out there have information on
(i) germinating plant (grass) pollen, and/or
(ii) outcrossing autogamous plants [e.g., treatments (chemical?) that kill
pollen but leave the egg alive and the stigma receptive to pollen]?
We are going to make F1 hybrids of goosegrass (Eleusine indica), which
reproduces predominantly by self-pollination.  We are trying to germinate
pollen in vitro, so that later we can confirm that we've killed it!  
Any help on the above two points will be appreciated.  Thanks
Vance_Baird@Quickmail.Clemson.EDU
803/656-4953 (-4960 FAX)
From: 101002.2366@CompuServe.COM ("Robert F. Whittier")
Newsgroups: bionet.genome.arabidopsis
Subject: reply: How much leaf for PCR?
Date: 7 Sep 92 02:08:32 GMT
Distribution: bionet

In response to Ulrich Melcher's question on the amount of leaf 
tissue to use in preparing DNA for PCR:

	We routinely obtain 500 ng to 1 ug of DNA from two leaves 
(about 20 mg) of fresh Arabidopsis tissue.  Since we use 10 ng of 
DNA in a 20 ul PCR reaction for single copy sequences, a 100 ul 
reaction could certainly be run with the DNA obtained from less 
than a mg of tissue.  If you want to standardize the amount of 
leaf tissue used without weighing it, you might try using the lid 
of  a microfuge tube to punch out a disc of leaf tissue into  the 
tube,  as  suggested by Edwards et al (1991)  Nucleic  Acids  
Research, Vol 19, p. 1349.

Bob Whittier
Mitsui Plant Biotechnology Research Institute
From: SLY2F@CC.USU.EDU
Newsgroups: bionet.genome.arabidopsis
Subject: subscribe to arabidopsis newsgroup
Date: 15 Sep 92 19:16:54 GMT
Distribution: bionet

Please add my name to the arabidopsis newsgroup.




 Thanks.
From: CHERRY@FRODO.MGH.HARVARD.EDU (Mike Cherry 726-5955)
Newsgroups: bionet.genome.arabidopsis
Subject: Re: Try the AAtDB Gopher
Date: 8 Sep 92 19:18:24 GMT
Distribution: bionet

I'd like to follow up on Chris Somerville's helpful message with two
comments about accessing the AAtDB Gopher and Wais services.

You will enjoy both Gopher and WAIS much more if you have a local
client.  While this is not required it would be very worthwhile to
obtain one of the clients. Gopher clients are available via anonymous
ftp from boombox.micro.umn.edu. Wais clients are available from
quake.think.com. Those who are using GopherApp by Don Gilbert on a
Macintosh can send me an email message and I'll send them a BinHexed
GopherApp document for the Arabidopsis Research Companion.

I know it can be difficult figuring out how to obtain and install
networking applications. The GOPHER account on ochre.mgh.harvard.edu
is provided to help make the Arabidopsis information more available to
the community. However this public access GOPHER account currently has
a limitation. You can only read the information via the terminal
screen, you cannot save the information to a file on your computer
unless your terminal emulator provides this feature (this is one of
the reasons to have Gopher or Wais client software).

An alternative public account can be used that allows any entry in the
WAIS server to be returned to you via electronic mail. Use the public
wais account on quake.think.com. You will need to "telnet
quake.think.com" from Unix or VMS.  On Macintosh and IBM compatibles
there are many different commands that provide telnet (PacerTerm,
VersaTerm, NCSA Telnet, ...).  As Chris pointed out in his message the
equivalient command on the MSU mainframe is tnvt.  Once connected to
quake.think.com enter the username wais (all lowercase), no password
is required. Then just follow the directions, or request help by
pressing the ? key at most any point. The AAtDB and Arabidopsis BioSci
WAIS servers are just two of about 300 WAIS databases available via
the Internet.

Mike Cherry

P.S. For those that need the IP number for networked computers and not
the hostname:
      ochre.mgh.harvard.edu            132.183.190.25
      quake.think.com                  192.31.181.1
From: kristoff@GENBANK.BIO.NET (Dave Kristofferson)
Newsgroups: bionet.genome.arabidopsis
Subject: ARAB-GEN test, please ignore
Date: 13 Sep 92 04:16:40 GMT
Distribution: bionet


This is a test.  Sorry for the bother.

Dave Kristofferson
From: BRIAN@BIO.TAMU.EDU
Newsgroups: bionet.genome.arabidopsis
Subject: Arabidopsis root culture
Date: 12 Sep 92 14:21:57 GMT
Distribution: bionet
Organization: Texas A&M University - Biology Dept

Does anyone have a good protocol for continuous in vitro culture of
Arabidopsis roots?.  I have grown cultures on a shaker from seed
germinated in MS liquid that are mostly root (the shoot remains
relatively small and misshapen in liquid), but when I subculture the
roots by themselves to a fresh flask they do not grow well.  I assume
the shoot is providing essential components to the root
(phytohormones?) that perhaps can be supplied exogenously.  Any
information will be appreciated.

Brian Taylor, Department of Biology, Texas A&M University
e-mail: Brian@bio.tamu.edu  Telephone (409)845-7754 FAX (-2891)
From: PLZMLH@VAX.CCC.NOTTINGHAM.AC.UK
Newsgroups: bionet.genome.arabidopsis
Subject: NASC change fax number
Date: 7 Sep 92 15:05:00 GMT
Distribution: bionet
Original-To: ARAB-GEN@UK.AC.DARESBURY


Please note that from September 7th 1992

The Nottingham Arabidopsis Stock Centre fax number will be
	
	0602 513251

Many thanks
Mary Anderson
From: kristoff@GENBANK.BIO.NET (Dave Kristofferson)
Newsgroups: bionet.genome.arabidopsis
Subject: Re: ARAB-GEN test, please ignore
Date: 13 Sep 92 04:34:12 GMT
Distribution: bionet

> 
> This is a test.  Sorry for the bother.
> 
> Dave Kristofferson
> 

I have received two reports of duplicate messages coming from the
ARABIDOPSIS mailing list but my test has so far not turned up anything
amiss here either with the news or mail distributions (nor has
anything been modified here recently so I am puzzled).  I'd appreciate
it if someone would send me two or three copies of what they received
including mail headers.

				Sincerely,

				Dave Kristofferson
				GenBank Manager

				kristoff@genbank.bio.net
From: 21847CRS@MSU.EDU ("Chris.Somerville")
Newsgroups: bionet.genome.arabidopsis
Subject: Try the AAtDB Gopher
Date: 8 Sep 92 04:27:00 GMT
Distribution: bionet


 I just tried out the AAtDB Gopher described earlier today by Mike Cherry and
I heartily recommend that everyone on the newsgroup take the time to figure
out how to use it.  It is extremely simple and very useful.  In particular, it
will give you access to all the old postings and the most current genetic
map(s) - including the YAC and cosmid contigs.  This is a tremendous service
to the community and I applaud Mike, Sam, Howard and colleagues for making it
available.

 Here are the steps I use to get on via a Western Digital ethernet connection
to my PC.

 Step 1. Type WD8003
         (This activates the program that permits communication with the
mainframe computer)

 Step 2. Type tnvt ochre.mgh.harvard.edu
         (tnvt.exe is a program that activates the telnet protocol. The
program may vary on your computer but it will look something like that.  The
result is that the MGH computer will ask you for the login (username) and
password.  Just type in ghopher and thaliana as per Mike's instructions and
you should be in and have a menu). If you get junk, type something like
alt-F10 then QUIT to close the connection.  OTherwise just follow
instructions.

 Have fun, Chris
From: kristoff@GENBANK.BIO.NET (Dave Kristofferson)
Newsgroups: bionet.genome.arabidopsis
Subject: Status of ARABIDOPSIS newsgroup
Date: 15 Sep 92 18:37:02 GMT
Distribution: bionet


Thanks to those who sent me copies of the repeated messages.  The
problem appears to have been with our mailer and it appears that the
mail system did not "believe" that the messages were correctly
received at remote sites.  This has not happened previously and
appears to have corrected itself as of Saturday afternoon.  Only the
ARABIDOPSIS mailing list appears to have been affected which is also
puzzling.  As we are extremely busy shutting down GenBank here, I am
not going to investigate this rather random event further unless it
recurs.

As was posted on bionet.announce/BIONEWS, our computer genbank.bio.net
will shut down after 29 September.  The genbank.bio.net host name will
be forwarded to our new machine which is called net.bio.net, so the
current posting address arab-gen@genbank.bio.net will continue to
work.  However, the official address after 29 Sept. will be

arab-gen@net.bio.net

and we recommend that you begin to use it in October if you particpate
in the ARABIDOPSIS group via e-mail.  The subscription
request/cancellation address will likewise change to 

biosci@net.bio.net

Please do not send subscription mail to the newsgroup or to my
personal e-mail address.

				Sincerely,

				Dave Kristofferson
				GenBank Manager

				kristoff@genbank.bio.net