From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Trichoderma harzianum alkaline proteinase (Prb1) gene, complete
Date: 2 Sep 92 10:01:38 GMT
Distribution: bionet
Checksum: 51093     6

LOCUS       TRRDBSCPRO   2449 bp ds-DNA             PLN       02-SEP-1992
DEFINITION  Trichoderma harzianum alkaline proteinase (Prb1) gene, complete
            cds.
ACCESSION   M87518 M87519
KEYWORDS    alkaline proteinase; secreted protein.
SOURCE      Trichoderma harzianum DNA.
  ORGANISM  Trichoderma harzianum
            Eukaryota; Fungi; Eumycota; Ascomycotina; Pyrenomycetes;
            Hypocreales; Hypocreaceae.
REFERENCE   1  (sites)
  AUTHORS   Geremia,R., Goldman,G., Jacobs,D., Vila,S., Ardiles,W.,
            Van Montagu,M. and Herrera-Estrella,A.
  TITLE     Purification and isolation of the gene of Prb1, an alkaline
            proteinase secreted by Trichoderma harzianum
  JOURNAL   Unpublished (1992)
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     CDS             join(425..724,783..1112,1186..1785)
                     /note="prepropeptide"
                     /product="alkaline proteinase"
                     /gene="Prb1"
                     /codon_start=1
                     /translation="MTSIRRLALYLGALLPAVLAAPAALHKKPEAVPNKFIVTLKEGA
                     SIDTDSHLAWVTDIHTRSLTKRSTAGVEKTYNIHTWNAYAGEFDEETIEQIKSNPDVA
                     SVEPDYIMHLSDIVEDKRALTTQSGAPWGLGTVSHRTSGSTSYIYDSSAGAGTFAYVV
                     DSGINTSHQQFGGRASLGYNAAGGQHVDTLGHGTHVSGTIGGSTYGVAKQASLISVKV
                     FAGESASTSVILDGYNWAVNDIVSKSRASKSAINMSLGGPASSTWTTAINAAFNQGVL
                     TIVAAGNGDSLGNPQPVSGTSPANVPNAITVAALDINWRTASFTNYGAGVDVFAPGVN
                     ILSSWIGSNTATNTISGTSMATPHVVGLALYLQSLEGLTSPTAVTNRIKALATSGRVT
                     GSLNGSPNVIIFNGNSS"
                     /partial
     exon            <425..724
                     /gene="Prb1"
     mat_peptide     join(843..1112,1186..1782)
                     /product="alkaline proteinase"
                     /gene="Prb1"
                     /codon_start=1
     sig_peptide     425..484
                     /gene="Prb1"
                     /codon_start=1
     intron          725..782
                     /gene="Prb1"
     exon            783..1112
                     /gene="Prb1"
     intron          1113..1185
                     /gene="Prb1"
     exon            1186..>1785
                     /gene="Prb1"
BASE COUNT      616 a    595 c    544 g    694 t
ORIGIN
        1 ggaatgatga aaagcaagac ggctattcat agaaatagac catgtattaa accatcttca
       61 tcgatatcca agttgatgcc ttggagatac tgcttcttca tcatgcttct ttccgcattc
      121 aaactagctg ccaagatctg ggcaccgtgg ctgccgtaac acaaacctat agtcttggtg
      181 tgatctattc gttctagttc tttttaggca gcttggatat ctatactcag cggcttggca
      241 agcaattaca atggagtgat tctggcagac gtgatggagg cttcagatag cgaaatcgag
      301 catatcatcc gcagtgacaa tgaaaactat ttaagttggc tgtattcgca agaatatctg
      361 gtaagttgga acagccatcg acttcctatc ttttctcctc cactatacaa agcacatcac
      421 agccatgacc agcattcgtc gtctcgctct ctatcttgga gctttgctcc cggctgtcct
      481 cgctgctcct gcagctcttc acaagaagcc tgaagccgta cccaacaagt tcatcgtcac
      541 tcttaaagag ggtgcttcta ttgataccga ctctcatctc gcctgggtaa cggacatcca
      601 cacccgttcg ttgaccaagc gtagcactgc tggtgttgaa aagacttata acattcatac
      661 ttggaatgct tatgcgggcg agtttgacga ggagacaatt gagcagatca agtccaaccc
      721 cgatgtacgt gtccgctgta ttgtgaagat aaaagaacaa aaagaagcta atcacactat
      781 aggttgcgtc tgttgagcca gactacatca tgcatctgtc ggacattgtt gaagacaagc
      841 gtgctctgac cacacaatct ggtgctcctt ggggtctcgg cactgtgtcc catcgcacat
      901 ctggatccac aagctacatt tatgatagct cagccggcgc cggcaccttt gcctatgtgg
      961 ttgactctgg tatcaacact tctcatcagc aattcggcgg gcgtgccagc cttggctaca
     1021 atgctgcagg aggacagcat gtcgatactc ttggccatgg tactcatgtt tccggaacaa
     1081 ttggtggatc tacatacggt gttgctaagc aggtgagcta ccctttgtcg actttttgtt
     1141 gtgaatacat gaatgcattc aagactaact cggtactgct cataggcgag cctaatctct
     1201 gtcaaggtct ttgctggtga aagtgcttcc acctctgtta tccttgacgg ctataactgg
     1261 gctgtaaacg acatcgtctc gaaaagccgt gctagcaagt ctgctattaa catgtcgctt
     1321 ggaggacctg cctcatctac ctggacgacc gccattaacg cagcctttaa ccagggcgtg
     1381 cttaccattg tcgccgctgg taatggcgac tctctgggaa acccccagcc agtctccggc
     1441 acttctcctg ctaacgttcc taacgccata accgttgccg cattggacat caactggcgc
     1501 actgcttcct tcaccaacta tggtgctggc gttgatgttt ttgcccctgg cgtcaacatt
     1561 ctgtcgtcat ggattggatc caacactgct accaacacaa tcagcggtac ttccatggct
     1621 acacctcacg ttgttggcct ggccctctat cttcaatctc tcgagggtct cacctctccg
     1681 accgctgtca ctaaccgaat taaagctctg gctacatctg gtcgtgtaac cggaagcctc
     1741 aacggcagcc ccaacgttat catcttcaac ggaaacagtt cttaaatcgt tgatggagcg
     1801 aaggatgaag tgaaatgttc atggaagggc agcacagctg ttgcttggct tgcttagctc
     1861 atttaagaag ctaatcacca tgatggagtg cgatgggtat ataagcttct cacagcgttt
     1921 tcaacttagc tagatgaccc tctatagatc gctaaaacaa aaagatatga cagatacctg
     1981 tgctttaatg tttcctgaca ctgtgtagtt cgttccaaaa cggcgaatct tgtagtattg
     2041 taactgtatg acatatatgt cctatcagga ttaggattag gattcggatt cggattatta
     2101 ttgctgaaaa ttagaatata atgattccct tttgatggct gtacagtcgg agggttttat
     2161 ttcccgcata taaagttata aacgctgtgc actcactagt ggttcaattc tacaaatcac
     2221 taaatttctg cgtttcaatg tataggtagt caagggcatt gcatatcata tgtaaatttt
     2281 catttcaatt tcgaaagtaa cgctaaagca gctctctata cttttgaact catccgcaat
     2341 ctatagttct aagacgcgct gagaatatgt agctcgcaga catgtattta gttgacaatc
     2401 ttcagggtct cgctgagggg aagcttctca gggacggtgg tgctgtggg
//