From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: E. coli DNA polymerase III delta subunit (holA) gene, complete cds.
Date: 3 Jan 93 22:05:03 GMT
Distribution: bionet
Checksum: 00370 4

LOCUS       ECODNAPDS    1147 bp ds-DNA             BCT       21-DEC-1992
DEFINITION  E. coli DNA polymerase III delta subunit (holA) gene, complete cds.
ACCESSION   M94267
KEYWORDS    DNA polymerase III delta subunit.
SOURCE      Escherichia coli (strain MAF102) DNA.
  ORGANISM  Escherichia coli
            Prokaryotae; Gram-negative facultatively anaerobic rods;
            Enterobacteriaceae.
REFERENCE   1  (bases 1 to 1147)
  AUTHORS   Takase,I., Ishino,F., Wachi,M., Kamata,H., Doi,M., Asoh,S.,
            Matsuzawa,H., Ohta,T. and Matsuhashi,M.
  TITLE     Genes encoding two lipoproteins in the leuS-dacA region of the
            Escherichia coli chromosome
  JOURNAL   J. Bacteriol. 169, 5692-5699 (1987)
  STANDARD  full automatic
REFERENCE   2  (bases 1 to 1147)
  AUTHORS   Carter,J.C., Franden,M.A., Aebersold,R.H. and McHenry,C.S.
  TITLE     Molecular cloning, sequencing, and overexpression of the structural
            gene encoding the delta subunit of Escherichia coli DNA polymerase
            III holoenzyme
  JOURNAL   J. Bacteriol. 174, 7013-7025 (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     RBS             38..43
                     /gene="holA"
     conflict        replace(79..107,"")
                     /citation=[1]
                     /note="the differences occur at position 735-761 of the
                     Takase paper; 'ctgctacgctcaatgaagggctgcggcc' in conflict"
     stem_loop       81..111
                     /gene="holA"
     CDS             55..1086
                     /function="accessory protein involved in replication"
                     /gene="holA"
                     /evidence="EXPERIMENTAL"
                     /citation=[2]
                     /product="DNA polymerase III delta subunit"
                     /codon_start=1
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAARAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQTQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
BASE COUNT      265 a    297 c    305 g    280 t
ORIGIN      
        1 cacaacggat actccggcaa cgcctgcacg cgtctccacc acgctgggta actgatgatt
       61 cggttgtacc cggaacaact ccgcgcgcag ctcaatgaag ggctgcgcgc ggcgtatctt
      121 ttacttggta acgatcctct gttattgcag gaaagccagg acgctgttcg tcaggtagct
      181 gcggcacaag gattcgaaga acaccacact ttttccattg atcccaacac tgactggaat
      241 gcgatctttt cgttatgcca ggctatgagt ctgtttgcca gtcgacaaac gctattgctg
      301 ttgttaccag aaaacggacc gaatgcggcg atcaatgagc aacttctcac actcaccgga
      361 cttctgcatg acgacctgct gttgatcgtc cgcggtaata aattaagcaa agcgcaagaa
      421 aatgccgcct ggtttactgc gcttgcgaat cgcagcgtgc aggtgacctg tcagacaccg
      481 gagcaggctc agcttccccg ctgggttgct gcgcgcgcaa aacagctcaa cttagaactg
      541 gatgacgcgg caaatcaggt gctctgctac tgttatgaag gtaacctgct ggcgctggct
      601 caggcactgg agcgtttatc gctgctctgg ccagacggca aattgacatt accgcgcgtt
      661 gaacaggcgg tgaatgatgc cgcgcatttc accccttttc attgggttga tgctttgttg
      721 atgggaaaaa gtaagcgcgc attgcatatt cttcagcaac tgcgtctgga aggcagcgaa
      781 ccggttattt tgttgcgcac attacaacgt gaactgttgt tactggttaa cctgaaacgc
      841 cagtctgccc atacgccact gcgtgcgttg tttgataagc atcgggtatg gcagaaccgc
      901 cggggcatga tgggcgaggc gttaaatcgc ttaagtcaga cgcagttacg tcaggccgtg
      961 caactcctga cacgaacgga actcaccctc aaacaagatt acggtcagtc agtgtgggca
     1021 gagctggaag ggttatctct tctgttgtgc cataaacccc tggcggacgt atttatcgac
     1081 ggttgatatg aaatctttac aggctctgtt tggcggcacc tttgatccgg tgcactatgg
     1141 tcatcta
//