From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: E.coli genes ubiC and ubiA
Date: 4 Sep 92 10:03:10 GMT
Distribution: bionet
Checksum: 20717     6

LOCUS       ECOUBI       2348 bp ds-DNA             BCT       04-SEP-1992
DEFINITION  E.coli genes ubiC and ubiA
ACCESSION   X66619
KEYWORDS    4-hydroxbenzoate-octaprenyltransferase; chorismate-pyruvate lyase;
            ubiA gene; ubiC gene.
SOURCE      Escherichia coli DNA.
  ORGANISM  Escherichia coli
            Prokaryotae; Gram-negative facultatively anaerobic rods;
            Enterobacteriaceae.
REFERENCE   1  (bases 1 to 2348)
  AUTHORS   Siebert,M., Bechthold,A., Melzer,M., May,U., Berger,U.,
            Schroeder,G., Schroeder,J.L., Severin,K. and Heide,L.
  TITLE     Ubiquinone biosynthesis Cloning of genes coding for chorismate
            pyruvate-lyase and 4-hydroxybenzoate octaprenyl transferase from
            escherichia coli
  JOURNAL   FEBS Lett. 307, 347-350 (1992)
  STANDARD  full automatic
COMMENT     *source: strain=MC4100; *source: clone_library=Sau3A partial
            digested DNA in pBluescript; *source: clone=pALMUI;
            See also K00127, M93136 & M93413
            From EMBL    entry ECUBI;  dated 10-AUG-1992.
FEATURES             Location/Qualifiers
     repeat_region   1994..2096
                     /note="palindrome"
     terminator      1965..1989
     misc_binding    1254..1322
                     /bound_moiety="polyprenyl"
     CDS             1080..1952
                     /product="4-hydroxybenzoate-octaprenyl transferase"
                     /gene="ubiA"
                     /codon_start=1
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSILVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     RBS             1068..1073
     CDS             570..1067
                     /product="chorismate-pyruvate lyase"
                     /gene="ubiC"
                     /codon_start=1
                     /translation="MSHPALTQLRALRYCKEIPALDPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     RBS             558..563
BASE COUNT      533 a    550 c    615 g    650 t
ORIGIN
        1 attcgtagtg atgatgtttc acctatattt atagaaactg aagataattt aagacccttt
       61 aaccacggaa tgaattaaca cgaaacgata atatctttat cctgttctcc tcagtcgatg
      121 atgggccagt tatggtggta agctcccagc gcttacatga tatgttgaat cctacaaaag
      181 ataccaattg gaattccacg tatatctaca aatccagaca tgagatgttg cctgttaatc
      241 ttactcagga aacacttttc agctccaaat ctcatggtaa atatgcgctt ttccccattt
      301 ttactgcgag ttggcgagct catcgtataa tgaataaggg tgtttaagta aaggaaaaca
      361 tcaccgttcc tggcatcctg gacggtgatg cccctacggt tgccctcgcc agcacgggca
      421 tcggtaaagc gtaaggttca acatcgtttt accacttcat gcgattgttg cgtttttgtt
      481 gcgtattaga tcacttaatt tgctttacat ctcccgtaaa cacttttctg cgatacaatg
      541 cctttacgtt atgtaacgga gagttcggca tgtcacaccc cgcgttaacg caactgcgtg
      601 cgctgcgcta ttgtaaagag atccctgccc tggatccgca actgctcgac tggctgttgc
      661 tggaggattc catgacaaaa cgttttgaac agcagggaaa aacggtaagc gtgacgatga
      721 tccgcgaagg gtttgtcgag cagaatgaaa tccccgaaga actgccgctg ctgccgaaag
      781 agtctcgtta ctggttacgt gaaattttgt tatgtgccga tggtgaaccg tggcttgccg
      841 gtcgtaccgt cgttcctgtg tcaacgttaa gcgggccgga gctggcgtta caaaaattgg
      901 gtaaaacgcc gttaggacgc tatctgttca catcatcgac attaacccgg gactttattg
      961 agataggccg tgatgccggg ctgtgggggc gacgttcccg cctgcgatta agcggtaaac
     1021 cgctgttgct aacagaactg tttttaccgg cgtcaccgtt gtactaagag gaaaaaaata
     1081 tggagtggag tctgacgcag aataagctgc tggcgtttca tcgcttaatg cgtacggata
     1141 agccaattgg cgcgttactg ctgctctggc caacattatg ggcgttgtgg gtggcgacac
     1201 cgggcgttcc ccagctctgg atcctggcgg tgtttgtcgc gggtgtctgg ctgatgcgcg
     1261 ctgccggatg tgtggtgaat gattatgctg accgcaagtt tgatggtcat gttaagcgca
     1321 cggcgaaccg accacttccc agcggcgcgg taacagagaa agaggcgcgc gcgctgtttg
     1381 tcgtgctggt actgatttcg tttttactgg tgctgacgct gaatacgatg accattctgt
     1441 tgtcgattgc cgcgctagcg ctggcgtggg tgtacccgtt tatgaagcgg tatacccatc
     1501 taccgcaagt ggtgctgggc gcggcgtttg gctggtcgat tccaatggct tttgccgctg
     1561 tgagtgagtc ggtgccattg agttgctggt taatgttcct cgccaatatt ctctgggcgg
     1621 tggcttacga cacgcagtat gcgatggttg accgcgatga tgatgtgaag attggcatta
     1681 aatccacggc aatcctgttc ggccaatacg ataaattgat tattggtatt ttgcagattg
     1741 gcgtactggc actgatggcg atcatcggtg agttaaatgg cttaggctgg ggatattact
     1801 ggtcaattct ggtggctggc gcgctgtttg tttatcaaca aaaactgatt gccaaccgcg
     1861 agcgtgaagc ctgctttaaa gcatttatga ataataacta tgttggtctg gtactatttt
     1921 tagggctggc aatgagttac tggcatttct gatgatgtaa aaaagccgga tgatcatccg
     1981 gctttcttct gggttgcctg atgcgcggcg cttctcaggc ctacacaaca catcgcaatt
     2041 tattgaattt gcagattatg gaaggccgga taaggcgttt tcgccgcatc cggcaattct
     2101 ctctgattac ccttcgccct gcgtcgcact ctcaatcgtc aaacgcacgt ctgatgtaat
     2161 caactccgcc agcaactgat aaaccttcat cgtttctgcc ggttcggcat cgccgctatc
     2221 gctgatatac ccttcatcac gcagtgtcag caccagagaa ctgaacaccg ccttgtcgaa
     2281 gaactccggc gcgttgatgc cgtgcagcac ggagagacgt tgcgcgacgg tgcggctctc
     2341 tttctcca
//