From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Bovine vacuolar H+-ATPase mRNA, complete cds.
Date: 9 Apr 92 10:02:20 GMT
Distribution: bionet
Checksum: 47464     6

LOCUS       BOVATPD      2573 bp ss-mRNA            MAM       09-APR-1992
DEFINITION  Bovine vacuolar H+-ATPase mRNA, complete cds.
ACCESSION   M88691
KEYWORDS    active proton transport; vacuolar H+-ATPase.
SOURCE      Bos taurus (library: lambda ZAP from Stratagene) adult kidney cDNA
            to mRNA.
  ORGANISM  Bos taurus
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Artiodactyla; Ruminantia; Pecora; Bovidae; Bovinae;
            Bovini.
REFERENCE   1  (bases 1 to 2573)
  AUTHORS   Nelson,R.D., Guo,X.-L., Masood,K., Brown,D., Kalkbrenner,M. and
            Gluck,S.
  TITLE     Selectively amplified expression of an isoform of the vacuolar
            H+-ATPase 56-kilodalton subunit in renl intercalated cells
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 89, 3541-3545 (1992)
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     5'UTR           1..125
     CDS             126..1667
                     /function="active proton transport"
                     /standard_name="H+ ATPase"
                     /note="kidney isoform, 56 kilodalton subunit"
                     /product="vacuolar H+-ATPase"
                     /codon_start=1
                     /translation="MAAEVDSRPRGLPGGGASLGAAREHVQAVTRNYITHPRITYRTV
                     CSVNGPLVVLDQVKFAQYAEIVNFTLPNGTQRSGQVLEVSGTKAIVQVFEGTSGIDAQ
                     KTTCEFTGDILRTPVSEDMLGRVFNGSGKPIDKGPVVMAEDFLDINGQPINPHDRIYP
                     EEMIETGISPIDVMNSIARGQKIPIFSAAGLPHNEIAAQICRQAGLVKKSKAVLDYHD
                     DNFAIVFAAMGVNMETARFFKSDFEQNGTMGNVCLFLNLANDPTIERIITPRLALTTA
                     EFLAYQCEKHVLVILTDMSSYAEALREVSAAREEVPGRRGFPGYMYTDLATIYERAGR
                     VEGRGGSITQIPILTMPNDDITHPIPDLTGFITEGQIYVDRQLHNRQIYPPINVLPSL
                     SRLMKSAIGEGMTRKDHGDVSNQLYACYAIGKDVQAMKAVVGEEALTSEDLLYLEFLQ
                     KFEKKFINQGPYEKRSVFESLDLGWKLLRTFPKEMLKRIPQNIIDEFFSREGAPQDTE
                     ADTAL"
     3'UTR           1665..2159
BASE COUNT      628 a    714 c    696 g    535 t
ORIGIN
        1 gaattccgga aaggagagga gggtggaaga aggggtggtt gcccgaggag gccggccggc
       61 tctctccagc tccagctcgg ctgagttgtg ggaggctcag gaacccgctc ccagcccgac
      121 acgccatggc cgcggaagta gacagtaggc ccaggggtct ccccggcgga ggcgcgagct
      181 tgggcgccgc ccgagaacac gtgcaggcgg tcacccgaaa ctacatcacc cacccccgca
      241 tcacctacag gaccgtgtgc agcgtcaatg ggcccctggt ggtgctggat caggtcaagt
      301 ttgcccagta tgctgagatt gtcaacttta ccctccccaa cgggactcag cggagtgggc
      361 aagtgctcga ggtgtctggg accaaggcaa ttgtgcaggt gtttgaaggg acctctggga
      421 ttgatgccca gaagaccacc tgcgaattca cgggggacat cctacggacc ccagtgtcag
      481 aggacatgct gggtcgggtt ttcaacggct caggcaaacc cattgacaag gggccagtgg
      541 tcatggcaga ggacttcctg gatatcaacg gccagcccat caacccccac gaccgcatct
      601 accccgagga gatgatcgag acaggcatct cgcccatcga cgtcatgaac agcattgccc
      661 gtggtcagaa gatccccatc ttctcagcag ctgggctccc ccacaatgag attgccgccc
      721 agatctgccg ccaggctggg ctggtgaaga agtccaaggc cgtgctggac tatcatgatg
      781 acaactttgc catcgtcttt gcagccatgg gggtgaacat ggagacagcc aggttcttca
      841 agtctgactt cgagcagaat ggcaccatgg gcaacgtctg cctcttcctg aacttggcca
      901 acgaccccac gatcgagcgg atcatcaccc cgcgcctggc actgaccacg gcagagttcc
      961 tcgcctacca gtgtgagaag cacgtgctgg tcatactgac ggacatgagt tcctatgcgg
     1021 aggccttgcg ggaagtctca gctgccagag aggaagtgcc tgggcgccga ggcttccctg
     1081 ggtacatgta cactgacttg gccaccatct acgagcgggc gggccgcgtg gagggccggg
     1141 gcggatccat cactcagatc cccatcctca ccatgcccaa tgacgatatc acccacccaa
     1201 tcccagacct gacgggcttc atcacagagg gacagatcta tgtggacaga cagcttcata
     1261 acagacagat ctacccgccc atcaacgtcc tcccttccct gtcgcggctg atgaagtccg
     1321 ccattgggga ggggatgacc agaaaggacc atggagatgt ctccaaccag ctgtatgcct
     1381 gctacgccat cgggaaggat gtgcaggcca tgaaggcagt ggtgggagag gaggcgctca
     1441 cttccgagga tctgctctac ctggaattcc tgcagaagtt tgagaagaag ttcatcaacc
     1501 agggtcccta cgagaaacgc tcggtgttcg agtctttgga cctgggctgg aagctgctgc
     1561 gcaccttccc caaggaaatg ctgaagcgca tcccgcagaa catcatcgat gaattctttt
     1621 cccgcgaggg ggcgccacag gacactgaag ccgacactgc gctgtagccc cgcccgccct
     1681 aggagcaggt ccagaccgcc ttcgtccgtc ccatctcacg gtccacacac tttaaccaac
     1741 tccgtgccca ccctcagccc acgggctatg aggagagagg aggccacttc tacattccag
     1801 tcctttccct ggattccccg aattgagaac cagaaggtgg ttaagcctgt attctctgtc
     1861 atgttctgat aggagcattt gtatattaaa gtaagccccc atcccataag aattcatctc
     1921 atgtaaaaat aagattggta aaacattgat gattgttgga gctgagggtg atggatttgt
     1981 gaggttcatt caactattct ctctacctta aaaagaaaaa acacattagt gtcttaacca
     2041 ataacagcat aattcttctt agggaaatat ttatcttcct taaaaagtat cttaataggt
     2101 atcttaggaa tgtgaggtca gggccataga agcagtctca aggccaccaa agatgcccgc
     2161 cttaagatat gttagaaagg aatttggttt actctaggga gttgctgggg atagcctgaa
     2221 gaaaagtgcc cccggttaac aaatgggtga agagcatcct aaaaaccagg atactgatgt
     2281 tcctttgaaa actgcccact cttctgtaat gccctccctt cctgcctcct ctgtcccatc
     2341 tacggtgaag tcatgaatgc ttcctaaaag gcagggaaag gcctggaagg tagatcttca
     2401 gcaaaagact gagtcccatt tctgggtcag tgtgttatgg aaccaggccg gaattcttgg
     2461 atcaagagct gaacccaggg acttccctgg cagtccagtg cttaggactt cggcttccaa
     2521 tgcaggggat gaagctgaga tcctgcatgc ctttccacca aaaaaaaaaa aaa
//