From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: G.max Gy4 gene for glycinin
Date: 21 Jul 92 10:08:29 GMT
Distribution: bionet
Checksum: 46136     7

LOCUS       SOYGY4       2942 bp ds-DNA             PLN       21-JUL-1992
DEFINITION  G.max Gy4 gene for glycinin
ACCESSION   X52863
KEYWORDS    glycinin.
SOURCE      Glycine max DNA.
  ORGANISM  Glycine max
            Eukaryota; Plantae; Embryobionta; Magnoliophyta; Magnoliopsida;
            Rosidae; Fabales; Fabaceae.
REFERENCE   1  (bases 1 to 2942)
  AUTHORS   Xue,Z.T., Xu,M.L., Shen,W., Zhuang,N.L., Hu,W.M. and Shen,S.C.
  TITLE     Characterization of the Gy4 Glycine gene from soybean Glycine max
            cv. Forrest
  JOURNAL   Plant Mol. Biol. 18, 897-908 (1992)
  STANDARD  full automatic
COMMENT     *source: strain=cv. Forrest; *source: tissue=leaf; *source:
            clone_library=Charon 4-EcoRI; *source: clone=lambda S312;
            From EMBL    entry GMGY4;  dated 12-JUN-1992.
FEATURES             Location/Qualifiers
     exon            <190..478
                     /number=1
     CDS             join(190..478,811..1073,1151..1897,2399..2788)
                     /product="glycinin"
                     /gene="Gy4"
                     /codon_start=1
                     /translation="MGKPFTLSLSSLCLLLLSSACFAISSSKLNECQLNNLNALEPDH
                     RVEFEGGLIQTWNSQHPELKCAGVTVSKLTLNRNGLHLPSYSPYPRMIIIAQGKGALQ
                     CKPGCPETFEEPQEQSNRRGSRSQKQQLQDSHQKIRHFNEGDVLVIPPGVPYWTYNTG
                     DEPVVAISLLDTSNFNNQLDQTPRVFYLAGNPDIEYPETMQQQQQQKSHGGRKQGQHQ
                     QEEEEEGGSVLSGFSKHFLAQSFNTNEDIAEKLQSPDDERKQIVTVEGGLSVISPKWQ
                     EQQDEDEDEDEDDEDEQIPSHPPRRPSHGKREQDEDEDEDEDKPRPSRPSQGKREQDQ
                     DQDEDEDEDEDQPRKSREWRSKKTQPRRPRQEEPRERGCETRNGVEENICTLKLHENI
                     ARPSRADFYNPKAGRISTLNSLTLPALRQFQLSAQYVVLYKNGIYSPHWNLNANSVIY
                     VTRGQGKVRVVNCQGNAVFDGELRRGQLLVVPQNFVVAEQAGEQGFEYIVFKTHHNAV
                     TSYLKDVFRAIPSEVLAHSYNLRQSQVSELKYEGNWGPLVNPESQQGSPRVKVA"
     mat_peptide     join(259..478,811..1073,1151..1897,2399..2785)
                     /product="glycinin"
                     /gene="Gy4"
                     /codon_start=1
     misc_feature    27..54
                     /note="legumin box, RY repeat element"
     TATA_signal     112..117
     sig_peptide     190..258
                     /gene="Gy4"
     intron          479..810
                     /number=1
     exon            811..1073
                     /number=2
     intron          1074..1150
                     /number=2
     exon            1151..1897
                     /number=3
     intron          1898..2398
                     /number=3
     exon            2399..>2788
                     /number=4
BASE COUNT      965 a    628 c    572 g    776 t      1 others
ORIGIN
        1 atgtatgagg tgtaacaaaa ttggaaacaa tagccatgca aggtgaagaa tgtcacaaac
       61 tcagcaaccc ttattcattg acgtgtccct cagtcactct cctctcatac ctataaatca
      121 ccactcctca tgttctttcc aattcaccaa ctccttcaaa cttaattatt aacacttcct
      181 tagttcaata tggggaagcc cttcactctc tctctttctt ccctttgctt gctactcttg
      241 tcgagtgcat gctttgctat tagctccagc aagctcaacg agtgccaact caacaacctc
      301 aacgcgttgg aacccgacca ccgcgttgag ttcgaaggtg gtttgattca aacatggaac
      361 tctcaacacc ctgagctgaa atgcgccggt gtcactgttt ccaaactcac cctcaaccgc
      421 aatggcctcc acttgccatc ttactcacct tatccccgga tgatcatcat cgcccaaggt
      481 aatcatatat aaggagtgct tctaacacac atatcagaaa gagtatcacc agcatttctc
      541 agtgtatatt aatccatttg tcaccacttg ttcaaatttc aacatcacat taccatagat
      601 catttactaa agataataat gatttaagta aatagtatct ctatagtaaa ttttacatga
      661 ttatttaact acaaattatt attatatata gaatgacttt gttgacatat caatcacctt
      721 aaaantttta ttaagttata tatatcaact aagatatctg acttaaataa aaatgtgagt
      781 gatctgcgcc tggtgatgag tgagtaccag ggaaaggagc actgcagtgc aagccaggat
      841 gtcctgagac gtttgaggag ccacaagaac aatcaaacag aagaggctca aggtcgcaga
      901 agcagcagct acaggacagt caccagaaga ttcgtcactt caatgaagga gacgtactcg
      961 tgattcctcc tggtgttcct tactggacct ataacactgg cgatgaacca gttgttgcca
     1021 tcagtcttct tgacacctct aacttcaata accagcttga tcaaacccct agggtaattc
     1081 atccaattta atttcattta ctattaacaa aacccctgtt ctccttactt gttaattctt
     1141 tcactttgag gtattttacc ttgctgggaa cccagatata gagtacccag agaccatgca
     1201 acaacaacaa cagcagaaaa gtcatggtgg acgcaagcag gggcaacacc agcaggagga
     1261 agaggaagaa ggtggcagcg tgctcagtgg cttcagcaaa cacttcttgg cacaatcctt
     1321 caacaccaac gaggacatag ctgagaaact tcagtctcca gacgacgaaa ggaagcagat
     1381 cgtgacagtg gaaggaggtc tcagcgttat cagccccaag tggcaagaac aacaagatga
     1441 agatgaagat gaagacgaag atgatgaaga tgaacaaatt ccctctcacc ctcctcgccg
     1501 accaagccat ggaaagcgtg aacaagacga ggacgaggac gaagatgaag ataaacctcg
     1561 tcctagtcga ccaagccaag gaaagcgtga acaagaccag gaccaggacg aggacgaaga
     1621 tgaagatgaa gatcaacctc gcaagagccg cgaatggaga tcgaaaaaga cacaacccag
     1681 aagacctaga caagaagaac cacgtgaaag aggatgcgag acaagaaacg gggttgagga
     1741 aaatatctgc accttgaagc ttcacgagaa cattgctcgc ccttcacgcg ctgacttcta
     1801 caaccctaaa gctggtcgca ttagtaccct caacagcctc accctcccag ccctccgcca
     1861 attccaactc agtgcccaat atgttgtcct ctacaaggta tgtaattcac ctcattcata
     1921 ttactaagta atcaacatga aactaattat acgtacatac ttacacatct aacagtaatt
     1981 tttccgtgga tattcaattg tcaattagtc tatcttgaga aaattaagaa ataaaaagaa
     2041 agcacaaaag ggaagcatct ttatgtcata aatcatatga tataataatt tagaagacat
     2101 ataaaaatgt cagtaagtat gttgtagggt tggattactt taaatgtcat taaaatatca
     2161 tttgatatgg gtaattcttt agtgattctc taggggtagt tgactgtaat gtattataat
     2221 tgtgcattga tttttatgag ttactttaac atgtcaatga agacttattt gataataatt
     2281 atagttactt gttggttcta ctacttttaa taaaaaaata ataaaaatat tggtgtaaat
     2341 atataatata taataataat gatgatgata cgtaacacat gttattatat ccatgcagaa
     2401 tggaatttac tctccacatt ggaatctgaa tgcaaacagt gtgatctatg tgactcgagg
     2461 acaaggaaag gttagagttg tgaactgcca agggaatgca gtgttcgacg gtgagcttag
     2521 gaggggacaa ttgctggtgg taccacagaa cttcgtggtg gcggagcaag ccggagaaca
     2581 aggattcgaa tacatagtat tcaagacaca ccacaacgca gtcactagct acttgaagga
     2641 tgtgtttagg gcaattccct cagaggttct tgcccattct tacaaccttc gacagagtca
     2701 agtgtctgag cttaagtatg aaggaaattg gggtcctttg gtcaaccctg agtctcaaca
     2761 aggctcaccc cgtgttaaag tcgcataaat gacaagcatg atggtgtaag gatgaggcca
     2821 tcttatgaaa taataacaaa taaataaatt ttgtatgata ataaaaagta tggcccatgt
     2881 ccctcccagc gagcctatgt ttatatctga gtggcgttgt acctttcaat cgccttaata
     2941 aa
//