From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human muscle phosphofructokinase (PFKM) mRNA, complete cds..
Date: 29 Sep 92 10:07:08 GMT
Distribution: bionet
Checksum: 30526     6

LOCUS       HUMPFKM23    2815 bp ss-mRNA            PRI       29-SEP-1992
DEFINITION  Human muscle phosphofructokinase (PFKM) mRNA, complete cds..
ACCESSION   M26066
KEYWORDS    phosphofructokinase.
SOURCE      Human (34 week old) placenta, cDNA to mRNA, clone HPFKM1.
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 2815)
  AUTHORS   Sharma,P.M., Reddy,G.R., Vora,S., Babior,B.M. and McLachlan,A.
  TITLE     Cloning and expression of a human muscle phosphofructokinase cDNA
  JOURNAL   Gene 77, 177-183 (1989)
  STANDARD  full staff_review
COMMENT     Draft entry and computer-readable sequence for [1] kindly submitted
            by P.M.Sharma, 12-JUL-1989.
FEATURES             Location/Qualifiers
     mRNA            <1..2815
                     /note="PFKM mRNA"
     CDS             77..2419
                     /EC_number="2.7.1.11"
                     /product="phosphofructokinase"
                     /gene="PFKM"
                     /codon_start=1
                     /translation="MTHEEHHAAKTLGIGKAIAVLTSGGDAQGMNAAVRAVVRVGIFT
                     GARVFFVHEGYQGLVDGGDHIKEATWESVSMMLQLGGTVIGSARCKDFREREGRLRAA
                     YNLVKRGITNLCVIGGDGSLTGADTFRSEWSDLLSDLQKAGKITDEEATKSSYLNIVG
                     LVGSIDNDFCGTDMTIGTDSALHRIMEIVDAITTTAQSHQRTFVLEVMGRHCGYLALV
                     TSLSCGADWVFIPECPPDDDWEEHLCRRLSETRTRGSRLNIIIVAEGAIDKNGKPITS
                     EDIKNLVVKRLGYDTRVTVLGHVQRGGTPSAFDRILGSRMGVEAVMALLEGTPDTPAC
                     VVSLSGNQAVRLPLMECVQVTKDVTKAMDEKKFDEALKLRGRSFMNNWEVYKLLAHVR
                     PPVSKSGSHTVAVMNVGAPAAGMNAAVRSTVRIGLIQGNRVLVVHDGFEGLAKGQIEE
                     AGWSYVGGWTGQGGSKLGTKRTLPKKSFEQISANITKFNIQGLVIIGGFEAYTGGLEL
                     MEGRKQFDELCIPFVVIPATVSNNVPGSDFSVGADTALNTICTTCDRIKQSAAGTKRR
                     VFIIETMGGYCGYLATMAGLAAGADAAYIFEEPFTIRDLQANVEHLVQKMKTTVKRGL
                     VLRNEKCNENYTTDFIFNLYSEEGKGIFDSRKNVLGHMQQGGSPTPFDRNFATKMGAK
                     AMNWMSGKIKESYRNGRIFANTPDSGCVLGMRKRALVFQPVAELKDQTDFEHRIPKEQ
                     WWLKLRPILKILAKYEIDLDTSDHAHLEHITRKRSGEAAV"
BASE COUNT      686 a    657 c    795 g    677 t
ORIGIN      310 bp upstream of PvuII site.
        1 cgggcctccc ccctttccca aggacaatct gcaagaaagc agcggcggag gagagctaag
       61 actaaaagag tggatcatga cccatgaaga gcaccatgca gccaaaaccc tggggattgg
      121 caaagccatt gctgtcttaa cctctggtgg agatgcccaa ggtatgaatg ctgctgtcag
      181 ggctgtggtt cgagttggta tcttcaccgg tgcccgtgtc ttctttgtcc atgagggtta
      241 tcaaggcctg gtggatggtg gagatcacat caaggaagcc acctgggaga gcgtttcgat
      301 gatgcttcag ctgggaggca cggtgattgg aagtgcccgg tgcaaggact ttcgggaacg
      361 agaaggacga ctccgagctg cctacaacct ggtgaagcgt gggatcacca atctctgtgt
      421 cattgggggt gatggcagcc tcactggggc tgacaccttc cgttctgagt ggagtgactt
      481 gttgagtgac ctccagaaag caggtaagat cacagatgag gaggctacga agtccagcta
      541 cctgaacatt gtgggcctgg ttgggtcaat tgacaatgac ttctgtggca ccgatatgac
      601 cattggcact gactctgccc tgcatcggat catggaaatt gtagatgcca tcactaccac
      661 tgcccagagc caccagagga catttgtgtt agaagtaatg ggccgccact gtggatacct
      721 ggcccttgtc acctctctgt cctgtggggc cgactgggtt tttattcctg aatgtccacc
      781 agatgacgac tgggaggaac acctttgtcg ccgactcagc gagacaagga cccgtggttc
      841 tcgtctcaac atcatcattg tggctgaggg tgcaattgac aagaatggaa aaccaatcac
      901 ctcagaagac atcaagaatc tggtggttaa gcgtctggga tatgacaccc gggttactgt
      961 cttggggcat gtgcagaggg gtgggacgcc atcagccttt gacagaattc tgggcagcag
     1021 gatgggtgtg gaagcagtga tggcactttt ggaggggacc ccagataccc cagcctgtgt
     1081 agtgagcctc tctggtaacc aggctgtgcg cctgcccctc atggaatgtg tccaggtgac
     1141 caaagatgtg accaaggcca tggatgagaa gaaatttgac gaagccctga agctgagagg
     1201 ccggagcttc atgaacaact gggaggtgta caagcttcta gctcatgtca gacccccggt
     1261 atctaagagt ggttcgcaca cagtggctgt gatgaacgtg ggggctccgg ctgcaggcat
     1321 gaatgctgct gttcgctcca ctgtgaggat tggccttatc cagggcaacc gagtgctcgt
     1381 tgtccatgat ggtttcgagg gcctggccaa ggggcagata gaggaagctg gctggagcta
     1441 tgttgggggc tggactggcc aaggtggctc taaacttggg actaaaagga ctctacccaa
     1501 gaagagcttt gaacagatca gtgccaatat aactaagttt aacattcagg gccttgtcat
     1561 cattgggggc tttgaggctt acacaggggg cctggaactg atggagggca ggaagcagtt
     1621 tgatgagctc tgcatcccat ttgtggtcat tcctgctaca gtctccaaca atgtccctgg
     1681 ctcagacttc agcgttgggg ctgacacagc actcaatact atctgcacaa cctgtgaccg
     1741 catcaagcag tcagcagctg gcaccaagcg tcgggtgttt atcattgaga ctatgggtgg
     1801 ctactgtggc tacctggcta ccatggctgg actggcagct ggggccgatg ctgcctacat
     1861 ttttgaggag cccttcacca ttcgagacct gcaggcaaat gttgaacatc tggtgcaaaa
     1921 gatgaaaaca actgtgaaaa ggggcttggt gttaaggaat gaaaagtgca atgagaacta
     1981 taccactgac ttcattttca acctgtactc tgaggagggg aagggcatct tcgacagcag
     2041 gaagaatgtg cttggtcaca tgcagcaggg tgggagccca accccatttg ataggaattt
     2101 tgccactaag atgggcgcca aggctatgaa ctggatgtct gggaaaatca aagagagtta
     2161 ccgtaatggg cggatctttg ccaatactcc agattcgggc tgtgttctgg ggatgcgtaa
     2221 gagggctctg gtcttccaac cagtggctga gctgaaggac cagacagatt ttgagcatcg
     2281 aatccccaag gaacagtggt ggctgaaact gaggcccatc ctcaaaatcc tagccaagta
     2341 cgagattgac ttggacactt cagaccatgc ccacctggag cacatcaccc ggaagcggtc
     2401 cggggaagct gccgtctaaa cctctctgga gtgaggggaa tagattacct gatcatggtc
     2461 agctcacacc ctaataagtc cacatcttct cagtgtttta gctgtttttt tcattaggtt
     2521 tccttttatt ctgtaccttg cagccatgac cagttctggc caggagctgg aggagcaggc
     2581 agtgggtggg agctcctttt aggtagaatt taacatgact tctgccccag ctttatctgt
     2641 cacacaaggc tgggcacctc tagtgctact gctagatatc acttactcag ttagaatttt
     2701 cctaaaaata agctttattt atttctttgt gataacaaag agtcttggtt cctctactac
     2761 ttttactaca gtgacaaatt gtaactacac taataaatgc caactggtca ctgtg
//