From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: E.coli K12 DNA with insertion element IS2, 5' end.
Date: 29 Aug 92 10:00:33 GMT
Distribution: bionet
Checksum: 26072     6

LOCUS       ECOINS2K     1442 bp ds-DNA             BCT       29-AUG-1992
DEFINITION  E.coli K12 DNA with insertion element IS2, 5' end.
ACCESSION   V00279 J01732 M18426
KEYWORDS    insertion element IS2; mobile genetic element; transposon.
SOURCE      E.coli (K12, strain HB101) DNA.
  ORGANISM  Escherichia coli
            Prokaryotae; Gram-negative facultatively anaerobic rods;
            Enterobacteriaceae.
REFERENCE   1  (bases 1 to 1442; 1 to 1442)
  AUTHORS   Ghosal,D., Sommer,H. and Saedler,H.
  TITLE     Nucleotide sequence of the transposable DNA-element IS2
  JOURNAL   Nucleic Acids Res. 6, 1111-1122 (1979)
  STANDARD  full staff_review
REFERENCE   2  (bases 66 to 1396)
  AUTHORS   Ronecker,H.-J. and Rak,B.
  TITLE     Genetic organization of insertion element IS2 based on a revised
            nucleotide sequence
  JOURNAL   Gene 59, 291-296 (1987)
  STANDARD  full staff_entry
COMMENT
            [2]  revises [1].
            Draft entry and computer-readable sequence for [2] kindly provided
            by B.Rak, 03-MAR-1988.
            The IS2 sequence is flanked by the gal operator promoter at the 5'
            end, and the galE sequence at the 3' end.
FEATURES             Location/Qualifiers
     transposon      66..1396
                     /note="insertion sequence IS2"
     CDS             complement(77..985)
                     /note="ORF2 (gtg start codon)"
                     /codon_start=1
                     /translation="KVDSARALIARGWGVSLVSRCLRVSRAQLHVILRRTDDWMDGRR
                     SRHTDDTDVLLRIHHVIGELPTYGYRRVWALLRRQAELDGMPAINAKRVYRIMRQNAL
                     LLERKPAVPPSKRAHTGRVAVKESNQRWCSDGFEFCCDNGERLRVTFALDCCDREALH
                     WAVTTGGFNSETVQDVMLGAVERRFGNDLPSSPVEWLTDNGSCYRANETRQFARMLGL
                     EPKNTAVRSPESNGIAESFVKTIKRDYISIMPKPDGLTAAKNLAEAFEHYNEWHPHSA
                     LGYRSPREYLRQRACNGLSDNRCLEI"
                     /partial
     old_sequence    replace(332
                     /citation=1
     CDS             506..937
                     /note="ORF4 (gtg start codon)"
                     /codon_start=1
                     /translation="MQCLTITAVQRERDTQSLSVITAELEPVRAPSLIAFFHGHSACM
                     CPFRWRYSRFSLKQQRILAHDPVNTFGIDRRHTIKFCLSAKQRPYPTITIRWQLSDNM
                     VYTEKHIRIISVTTAAAIHPVIGSSENDVQLRTRHPETTAD"
     CDS             complement(616..912)
                     /note="ORF3 (gtg start codon)"
                     /codon_start=1
                     /translation="RVRSCTSFSDEPMTGWMAAAVVTLMIRMCFSVYTMLSESCQRMV
                     IVGYGRCFADRQNLMVCLRSMPNVFTGSCARMRCCLSENLLYRHRNGHIQAEWP"
                     /partial
     CDS             complement(940..1308)
                     /note="ORF1"
                     /codon_start=1
                     /translation="QMIDVLGPEKRRRRTTQEKIAIVQQSFEPGMTVSLVARQHGVAA
                     SQLFLWRKQYQEGSLTAVAAGEQVVPASELAAAMKQIKELQRLLGKKTMENELLKEAV
                     EYGRAKKWIAHAPLLPGDGE"
                     /partial
     old_sequence    replace((975.977)..(975.977)
                     /citation=1
     old_sequence    replace((1033.1034)..(1033.1034)
                     /citation=1
     old_sequence    replace((1128.1130)..(1128.1130)
                     /citation=1
     old_sequence    replace((1198.1200)..(1198.1200)
                     /citation=1
     old_sequence    replace((1229.1231)..(1229.1231)
                     /citation=1
     old_sequence    replace((1289.1291)..(1289.1291)
                     /citation=1
BASE COUNT      312 a    412 c    336 g    382 t
ORIGIN      1 bp upstream of HinfI site.
        1 gattccacta atttattcca tgtcacactt ttcgcatctt tgttatgcta tggttatttc
       61 atacctggat ttgcccctat atttccagac atctgttatc acttaaccca ttacaagccc
      121 gctgccgcag atattcccgt ggcgagcgat aacccagcgc actatgcgga tgccattcgt
      181 tataatgctc gaacgcctct gcaaggttct ttgctgccgt taacccgtct ggtttgggca
      241 tgatactgat gtagtcacgc tttatcgttt tcacgaagct ctctgctatt ccgttactct
      301 ccggactccg caccgccgtg ttcttcggtt caagtcccaa catccgggcg aactggcgtg
      361 tttcattagc ccggtagcat gaaccattat ccgtcagcca ctccactgga gacgacggaa
      421 gatcgttgcc gaagcggcgt tccaccgctc ccagcatgac gtcctgtact gtttcactgt
      481 tgaagccgcc ggtagtgacc gcccagtgca gtgcctcacg atcacagcag tccagcgcga
      541 acgtgacacg cagtctctct ccgttatcac agcagaactc gaacccgtca gagcaccatc
      601 gctgattgct ttctttcacg gccactctgc ctgtatgtgc ccgtttcgat ggcggtacag
      661 caggttttcg ctcaagcaac agcgcattct ggcgcatgat ccggtaaaca cgtttggcat
      721 tgatcgcagg cataccatca agttctgcct gtctgcgaag cagcgcccat acccgacgat
      781 aaccatacgt tggcagctct ccgataacat ggtgtatacg gagaagcaca tccgtatcat
      841 cagtgtgacg actgcggcgg ccatccatcc agtcatcggt tcgtctgaga atgacgtgca
      901 actgcgcacg cgacacccgg agacaacggc tgactaagct tactccccat ccccgggcaa
      961 taagggcgcg tgcgctatcc acttttttgc ccgtccatat tcaacggctt ctttgaggag
     1021 ttcattttcc atcgttttct tgccgagcag gcgctggagt tctttaatct gcttcatggc
     1081 ggcagcaagt tcagaggcag gaacaacctg ttctccggcg gcgacagcag taagacttcc
     1141 ttcctggtat tgcttacgcc agagaaataa ctggctggct gctacaccat gttgccgggc
     1201 aacgagggag accgtcatcc ccggttcaaa gctctgctga acaattgcga tcttttcctg
     1261 tgtggtacgc cgtctgcgtt tctccggccc taagacatca atcatctgtt ctccaatgac
     1321 tagtctaaaa actagtatta agactatcac ttatttaagt gatattggtt gtctggagat
     1381 tcagggggcc agtctaatac cataagccta atggagcgaa ttatgagagt tctggttacc
     1441 gg
//