From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: C.freundii GN346 class C beta-lactamase gene
Date: 3 Jan 93 22:04:03 GMT
Distribution: bionet
Checksum: 56421 4

LOCUS       CFRBLCAA     1408 bp ds-DNA             BCT       21-DEC-1992
DEFINITION  C.freundii GN346 class C beta-lactamase gene
ACCESSION   X51632
KEYWORDS    beta-lactamase; cephalosporinase.
SOURCE      Citrobacter freundii DNA.
  ORGANISM  Citrobacter freundii
            Prokaryotae; Gram-negative facultatively anaerobic rods;
            Enterobacteriaceae.
REFERENCE   1  (bases 1 to 1408)
  AUTHORS   Tsukamoto,K., Tachibana,K., Yamazaki,N., Ishii,Y., Ujiie,K.,
            Nishida,N. and Sawai,T.
  TITLE     Role of lysine-67 in the active site of class C beta-lactamase from
            Citrobacter freundii GN346
  JOURNAL   Eur. J. Biochem. 188, 15-22 (1990)
  STANDARD  full automatic
COMMENT     *source: strain=GN346;
               From EMBL    entry CFBLCAA;  dated 17-DEC-1992.
FEATURES             Location/Qualifiers
     -35_signal      23..28
     -10_signal      46..51
     terminator      1295..1318
     CDS             143..1288
                     /note="The active site of this class Cbeta-lactamase was
                     confirmed to be Ser-64. Theprotein also exhibits the
                     substrate profile of atypical cephalosporinase"
                     /product="beta-lactamase"
                     /codon_start=1
                     /translation="MMKKSICCALLLTASFSTFAAAKTEQQIADIVNRTITPLMQEQA
                     IPGMAVAIIYEGKPYYFTWGKADIANNHPVTQQTLFELGSVSKTFNGVLGGDAIARGE
                     IKLSDPVTKYWPELTGKQWRGISLLHLATYTAGGLPLQIPDDITDKAALLRFYQNWQP
                     QWTPGAKRLYANSSIGLFGALAVKPSGMSYEEAMTRRVLQPLKLAHTWITVPQSEQKN
                     YAWGYREGKPVHVSPGQLDAEAYGLKSSVIDMARWVQANMDASHVQEKTLQQGIELAQ
                     SRYWRIGDMYQGLGWEMLNWPLKADSIINGSDSKVALAALPAVEVNPPVPAVKASWVH
                     KTGSTGGFGSYVAFVPEKNLGIVMLANKSYPNPVRVEAAWRILEKLQ"
BASE COUNT      362 a    373 c    357 g    316 t
ORIGIN      
        1 gtacgcgtca tcatccggcc tgttagaaaa acttatatct gctgctaaat ttaaccgttt
       61 gtcaacacgg tgcaaatcaa acacactgat tgcgtctgac gggcccggac acccttttgc
      121 ttttaattac ggaactgatt tcatgatgaa aaaatcgata tgctgcgcac tgctgctgac
      181 agcctctttc tccacgtttg ctgccgcaaa aacagaacaa caaattgccg atatcgttaa
      241 ccgcaccatc acaccactga tgcaggagca ggctattccg ggtatggccg tggcgattat
      301 ctacgagggg aaaccttatt actttacctg gggtaaagcc gatatcgcca ataaccaccc
      361 agtcacgcag caaacgctgt ttgagctagg gtcggtcagt aagacgttta acggcgtgtt
      421 gggcggcgac gctatcgccc gcggcgaaat taagctcagc gatccggtca cgaaatactg
      481 gccagaactg acaggcaaac agtggcgggg tatcagcctg ctgcacttag ccacctatac
      541 agcgggtggc ctgccgctgc agatccccga tgacattacg gataaagccg cattactgcg
      601 cttttatcaa aactggcaac cacaatggac tccgggcgct aagcgtcttt acgctaactc
      661 cagcattggt ctgtttggtg cgctagcggt gaaaccttca ggtatgagct acgaagaggc
      721 aatgaccaga cgcgtcctgc aaccattaaa actggcgcat acctggatta cggttccgca
      781 aagcgaacaa aaaaactatg cctggggcta tcgcgaaggg aagcctgtgc acgtttctcc
      841 gggacaactt gacgccgaag cctatggcct gaaatccagc gttatcgata tggcccgctg
      901 ggttcaggcc aacatggacg ccagccacgt tcaggagaaa acgctccagc agggcattga
      961 gcttgcgcag tctcgctact ggcgtattgg tgatatgtac cagggattag gctgggagat
     1021 gctgaactgg ccgctgaaag ctgattcgat catcaacggc agcgacagca aagtggcatt
     1081 ggcagcgctt cccgccgttg aggtaaaccc gccagtacct gccgtgaaag cctcatgggt
     1141 gcataaaacg ggatccacag gtggatttgg cagctacgtt gccttcgttc cagaaaaaaa
     1201 ccttggcata gtgatgctgg caaacaaaag ctatcctaac cctgtccgcg tcgaggcggc
     1261 ctggcgcatt cttgaaaaac tgcaataact gacagagggg tccggtaaat cggacctcct
     1321 ttcttgctct ttttttcctg ctgtcatcta tacttaacaa aaaacagtaa ggaaaacact
     1381 atgccgattc tgcccgtcgt tgctgcag
//