From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: M.musculus 26-10V kappa gene for anti-digoxin antibody
Date: 21 Dec 92 20:49:31 GMT
Distribution: bionet
Checksum: 22856 4

LOCUS       MUSADANBO    1055 bp ds-DNA             ROD       18-NOV-1992
DEFINITION  M.musculus 26-10V kappa gene for anti-digoxin antibody
ACCESSION   X56623
KEYWORDS    anti-digoxin antibody.
SOURCE      Mus musculus DNA.
  ORGANISM  Mus musculus
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Rodentia; Myomorpha; Muridae; Murinae.
REFERENCE   1  (sites)
  AUTHORS   Near,R.I., Ng,S.C., Mudgett-Hunter,M., Hudson,N.W., Margolies,M.N.
            and Seidman,J.G.
  TITLE     Heavy and light chain contributions to antigen binding in an
            anti-digoxin chain recombinant antibody produced by transfection of
            cloned anti-digoxin antibody genes.
  JOURNAL   Mol. Immunol. 27, 901-909 (1990)
  STANDARD  full automatic
REFERENCE   2  (bases 1 to 1055)
  AUTHORS   Anthony,J., Near,R.I., Wong,S.L., Iida,E., Ernst,E., Wittekind,M.,
            Haber,E. and Ng,S.C.
  TITLE     Production of stable anti-digoxin Fv in escherichia coli
  JOURNAL   Mol. Immunol. 29, 1237-1247 (1992)
  STANDARD  full automatic
COMMENT     From EMBL    entry MMADANBO;  dated 29-OCT-1992.
FEATURES             Location/Qualifiers
     exon            <193..238
                     /number=1
     intron          239..620
                     /number=1
     exon            621..968
                     /number=2
     intron          969..>1055
     CDS             join(193..238,621..968)
                     /partial
                     /partial
                     /product="anti-digoxin antibody"
                     /codon_start=1
                     /translation="MKLPVRLLVLMFWIPASSSDVVMTQTPLSLPVSLGDQASISCRS
                     SQSLVHSNGNTYLNWYLQKAGQSPKLLIYKVSNRFSGVPDRFSGSGSGTDFTLKISRV
                     EAEDLGIYFCSQTTHVPPTFGGGTKLEIK"
BASE COUNT      290 a    210 c    245 g    310 t
ORIGIN      
        1 ggttttgtgt cacttttaaa atcttaacta gtctatataa ctcttccttc tatactgcaa
       61 cactaaaggg agcttttgca tattgctccc cagggagggc ccacccttac tagtctgaga
      121 taaaagctca gctgtcttgt ttcagtgact gatcagtctc ctcaggctgt ctcctcaggt
      181 tgcctcctca aaatgaagtt gcctgttagg ctgttggtgc tgatgttctg gattcctggt
      241 gaggagagag ggaagtgagg gaggagaatg gacagggagc aggagcactg aatcccattg
      301 ctcattccat gtattctgcc atgggtgaga agatgggtct tatcctccag catggggcct
      361 ctggggtgaa tacttgttag agggaggttc cagatgggaa catgtgctat aatgaagatt
      421 atgaaatgga tgcctgggat ggtctaagta atgcctagaa gtgactagac acttgcaatt
      481 cacttttttt ggtaagaaga gaattttagg atataaaaaa atggtatgta aaaataaaca
      541 tcacagttga aataaaaaaa aaacataagg atgttcatga attttgtgtg ttagtatgta
      601 tttctctctc attatttcag cttccagcag tgatgttgtg atgacccaaa ctccactctc
      661 cctgcctgtc agtcttggag atcaagcctc catctcttgc agatctagtc agagccttgt
      721 acacagtaat ggaaatacct atttaaattg gtacctgcag aaggcaggcc agtctccaaa
      781 gctcctgatc tacaaagttt ccaaccgatt ttctggggtc ccagacaggt tcagtggcag
      841 tggatcaggg acagatttca cactcaagat cagcagagtg gaggctgaag atctgggaat
      901 ttatttctgc tctcaaacta cacatgttcc tccgacgttc ggtggaggca ccaagctgga
      961 aatcaaacgt aagtagaatc caaagtctct ttcttccgtt gtctatgtct gtggcttcta
     1021 tgtctaaaaa tgatgtataa aatcttactc tgaaa
//