HDTenEightyP/NetNews huggingface dataset:
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human immunodeficiency virus type 2 (FOENVAD7) envelope protein
Date: 18 Aug 92 10:01:47 GMT
Distribution: bionet
Checksum: 48236     2

LOCUS       HIVENVAD7     453 bp ss-RNA             VRL       18-AUG-1992
DEFINITION  Human immunodeficiency virus type 2 (FOENVAD7) envelope protein
            fragment.
ACCESSION   M87081
KEYWORDS    envelope protein.
SOURCE      Human immunodeficiency virus type 2 uncultured blood mononuclear
            cells (11/89 sample) RNA.
  ORGANISM  Human immunodeficiency virus type 2
            Viridae; ss-RNA enveloped viruses; Positive strand RNA virus;
            Retroviridae; Lentivirinae.
REFERENCE   1  (bases 1 to 453)
  AUTHORS   Gao,F., Yue,L., White,A.T., Pappas,P.G., Barchue,J., Hanson,A.P.,
            Greene,B.M., Sharp,P.M., Shaw,G.M. and Hahn,B.H.
  TITLE     Human infection by genetically-diverse SIVsm-related HIV-2 in West
            Africa
  JOURNAL   Nature (1992) In press
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     misc_feature    1..453
                     /note="PCR derived HIV-2 envelope fragment"
                     /gene="env"
                     /note="putative"
                     /citation=[1]
BASE COUNT      183 a     77 c     83 g    110 t
ORIGIN
        1 aacgtggtca aaagacaaca aaaattgttg cgactaaccg tctgggagac taaaaacctc
       61 cagactaaag tcactgctat caaaaagtac ctaaagaacc aggcacagct aaattcatag
      121 gaatgcgcgt tcagacaggt ctgccacacc actgtaccat ggccaaataa tagtctcaca
      181 cctaactgaa acaatataac ttggcagaag tagaaaagaa aggttaattt cctaaaggca
      241 aatataacac agatgttaaa gaaagcacaa attcaacagg agaaaaacat gtataagtta
      301 caaaaactaa ataactggga tatatttggc aactggtttg accttacttc ttggattaag
      361 tatatacagt atggagtctt catagtagta ggtataatac tgttaagaat aataatctat
      421 gtagtacaaa tgttagctaa gttaagtaag gga
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human Ig germline H-chain gene V region, partial cds.
Date: 22 Aug 92 10:00:41 GMT
Distribution: bionet
Checksum: 25061     2

LOCUS       HUM7IGVH      434 bp ds-DNA             PRI       22-AUG-1992
DEFINITION  Human Ig germline H-chain gene V region, partial cds.
ACCESSION   M99403
KEYWORDS    immunoglobulin heavy chain subgroup VH-III;
            immunoglobulin heavy chain variable region.
SOURCE      Homo sapiens (individual_isolate LS) blood DNA.
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 434)
  AUTHORS   Adderson,E.E., Carroll,W.L. and Shackelford,P.G.
  TITLE     The human VH3b gene subfamily is highly polymorphic
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     CDS             join(1..13,117..217)
                     /product="immunoglobulin heavy chain variable region"
                     /gene="IGHV@"
                     /codon_start=1
                     /translation="AILKGVQCEVQLVESGGGLVKPGGSLRLSCAASGFTFS"
                     /partial
     sig_peptide     1..13
                     /gene="IGHV@"
                     /codon_start=1
                     /partial
     intron          14..116
                     /gene="IGHV@"
BASE COUNT      112 a     83 c    135 g    104 t
ORIGIN
        1 gctattttaa aaggtgattt atggagaact agagagatta agtgtgagtg gacgtgagtg
       61 agagagacag tggatatgtg tggcagtttc tgatcttagt gtctctgtgt ttgcaggtgt
      121 ccagtgtgag gtgcagctgg tggagtctgg gggaggcttg gtaaagcctg gggggtccct
      181 tagactctcc tgtgcagcct ctggattcac tttcagtaac gcctggatga gctgggtccg
      241 ccaggctcca gggaaggggc tggagtgggt tggccgtatt aaaagcaaaa ctgatggtgg
      301 gacaacagac tacgctgcac ccgtgaaagg cagattcacc atctcaagag atgattcaaa
      361 aaacacgctg tatctgcaaa tgaacagtct gaaaaccgag gacacagccg tgtattactg
      421 taccacagac acag
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human gamma-aminobutyric acid receptor A5 subunit (GABRA5) gene.
Date: 20 Aug 92 10:03:37 GMT
Distribution: bionet
Checksum: 64430     2

LOCUS       HUMGABRA5X    323 bp ds-DNA             PRI       20-AUG-1992
DEFINITION  Human gamma-aminobutyric acid receptor A5 subunit (GABRA5) gene.
ACCESSION   M93435
KEYWORDS    gamma-aminobutyric acid A receptor.
SOURCE      Homo sapiens (library: EMBL3) adult DNA.
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 323)
  AUTHORS   Glatt,K.A., Sinnett,D. and Lalande,M.
  TITLE     Dinucleotide repeat polymorphism at the GABAa receptor A5 (GABRA5)
            locus of charomosome 15q11q13
  JOURNAL   Hum. Mol. Genet. 1, 348-348 (1992)
  STANDARD  full staff_entry
FEATURES             Location/Qualifiers
     repeat_region   61..146
                     /gene="GABRA5"
BASE COUNT       65 a     55 c     72 g    130 t      1 others
ORIGIN
        1 ggtcaacgga tctttatatg tttggtagaa tttccctgta aaggcacctg ggccctttgt
       61 ctctctctct ttctctctct ctctctctgt gtgtgtgtgc gtgcgtgtgt gtgtgtgtgt
      121 gtgtgtgtgt gtgtgtgtgt gtgtgtaggc gagtaaaagt tccttggtaa ttttctcttt
      181 ttccatagca aatggcccct tancattcta tctataatgg ggtcaatttc agtagtaaaa
      241 tatatttccc caagaaattc tccaatatgt atatgttcta aattttttga ataaaggtgg
      301 gtaagtcatc tgttatgact tta
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human chromosome 4 sequence-tagged site STS4-456
Date: 21 Aug 92 10:10:36 GMT
Distribution: bionet
Checksum: 11204     3

LOCUS       HUM4STS456    432 bp ds-DNA             PRI       21-AUG-1992
DEFINITION  Human chromosome 4 sequence-tagged site STS4-456
ACCESSION   L00872
KEYWORDS    STS; human chromosome 4; sequence tagged site.
SOURCE      Homo sapiens, clone p4-505 from a chromosome 4 plasmid library;
            vector pBluescript II KS + (Stratagene)
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 432)
  AUTHORS   Goold,R.D., diSibio,G., Dugaiczyk,A., Smith,K.A., Xu,H.,
            Magrane,G., Lang,D.B., Cox,D.R., Masters,S.B. and Myers,R.M.
  TITLE     Sequence-Tagged Sites for Human Chromosome 4
  JOURNAL   Unpublished (1992) see COMMENT for author address
  STANDARD  full automatic
COMMENT     PCR components: 25 ng of human genomic DNA, 10 pmol of each
            oligonucleotide, 200 micro-M dNTPs, 0.25 U Taq  polymerase (Cetus)
            in 10 micro-l of 50 mM KCl-20 mM  Tris-HCl, pH 8.3 (at room temp),
            2.5 mM MgCl-2.  Initial denaturation at 94degC for 1.5 min, then 30
            cycles of  94degC for 15 sec, 62degC for 23 sec, and 72degC for 30
            sec, followed by a final extension at 72degC for 3.5 min, using a
            Perkin-Elmer 9600 thermocycler. PCR-amplified product size 295 bp.
            Sequence submitted by:
            Human Genome Mapping Center
            Box 0925
            University of California San Francisco
            San Francisco, CA 94143-0925  USA
            Phone: (415) 502-1612   Fax: (415) 476-8391
            e-mail: hgmcprobes@cgl.ucsf.edu
FEATURES             Location/Qualifiers
     misc_feature    5..27
                     /label=Primer1
     misc_feature    complement(274..299)
                     /label=Primer2
BASE COUNT      102 a    111 c     88 g    130 t      1 others
ORIGIN
        1 gatctacaga ttgcagaaac cgtgggaaaa gcatagtaac cagtccctca cagctttcct
       61 tggctgtggg agggagttcc ctgactcctt gcacttcctg ggtaaagcga caccccaccc
      121 tacttctgtt catcctccat gggttggacc cactgcctaa ccagtcccga tgagatgaac
      181 tgagtacctc agttggaaat gcagaaatca tcttccttct gcgttggtct tgctgggagc
      241 tgcagaccgg agctgttcct gtttggctat ttactacctg aaattattta tcttcttgct
      301 tattatttgt cttttccact agaatgtgtg agagcaggac ccttgtctct tctaccactc
      361 tatacctacc accactgaac agngcttggt attcattaaa ctgctcaata aatattgaag
      421 gaatgatttc ac
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human zinc finger protein 20 (ZNF20) pentanucleotide repeat
Date: 22 Aug 92 10:01:02 GMT
Distribution: bionet
Checksum: 51527     3

LOCUS       HUMZNF20X    1226 bp ds-DNA             PRI       22-AUG-1992
DEFINITION  Human zinc finger protein 20 (ZNF20) pentanucleotide repeat
            polymorphism.
ACCESSION   M99593
KEYWORDS    pentanucleotide repeat polymorphism; zinc finger protein 20.
SOURCE      Homo sapiens DNA.
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 1226)
  AUTHORS   Antonarakis,S.E. and Chen,H.
  TITLE     Zinc finger gene with a pentanucleotide repeat polymorphism on
            human chromosome 19p
  JOURNAL   Unpublished (1992)
  STANDARD  simple staff_entry
BASE COUNT      414 a    215 c    230 g    367 t
ORIGIN
        1 gaattccgtt gatcagtcac atcttcaggc aaataggata actcacaatg gagaaacact
       61 ctatgaacag aagcaatgtg ggagagcttt tacttactcc acaagccatg ctgtgtctgt
      121 taaaatgcat actgtagaaa aaccctacga atgtaaggaa tgtggaaaat tctttagata
      181 ttcttcatat cttaatagtc acatgcgaac ccatactggg gagaaaccct atgaatgtaa
      241 ggaatgtggg aaatgcttca ctgtttcttc acacctagtt gaacatgtaa gaatccatac
      301 tggagagaaa ccttatcaat gtaaggaatg tggaagagcc ttcgctgggt gttcaggcct
      361 tactaaacat gtacgaatac acactggaga gaagccctat gaatgtaacg aatgtgggaa
      421 agcctacaat aggttttatc tactaactga acattttaaa actcacacag aggagaagcc
      481 ctttgaatgt aaggtatgtg gaaaatcctt cagaagctct tcatgcctta agaatcactt
      541 tagaattcac actggaataa aaccctataa atgtaaggag tgtgggaaag cattcactgt
      601 ttcctcaagc ttacataatc atgtaaaaat ccatactgga gagaagccct atgaatgtaa
      661 ggactgtggg aaagcctttg ctacatcttc acaactcatt gaacatataa gaactcacac
      721 tggagagaaa ccgtatatat gtaaggaatg tgggaaaacc ttccgtgctt cttcacatct
      781 acagaaacat gttagaattc acactggaga gaaaccctat atatgtaacg aatgtgggaa
      841 agcctacaat agattttatt tacttactaa acatttaaaa acacactgaa gaaaaactct
      901 gaatgtaagg aatgtggaaa tcttttagga atttctcata ccataattac cacatttgaa
      961 ttcacactga agagaaattt tattggtaca aagaatgtga ggaggccttc agttatcccc
     1021 attcacttcg aagacatgaa agaactcata ctctatgtgt cccctataaa ttttaggaat
     1081 gttagggagc caatggaatc ttttttttat tattttattt tattttattt tattttattt
     1141 tattttattt tattttattt tatttgagac agagttttgc tgttgtcatc acacaggctg
     1201 gagtgcaatg gcatgatccg gaattc
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Mouse VPre B1 gene promoter
Date: 15 Aug 92 10:02:26 GMT
Distribution: bionet
Checksum: 54387     3

LOCUS       MUSBPBPRO     817 bp ds-DNA             ROD       15-AUG-1992
DEFINITION  Mouse VPre B1 gene promoter
ACCESSION   X64930
KEYWORDS    Ig light chain v-region; light chain-homologue;
            pre-B cell receptor.
SOURCE      Mus musculus DNA.
  ORGANISM  Mus musculus
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Rodentia; Myomorpha; Muridae; Murinae.
REFERENCE   1  (bases 1 to 817)
  AUTHORS   Kudo,A.
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
REFERENCE   2  (bases 1 to 817)
  AUTHORS   Okabe,T., Bauer,S.R. and Kudo,A.
  TITLE     Pre-B lymphocyte-specific transcriptional control of the mouse V
            preB gene
  JOURNAL   Eur. J. Immunol. 22, 31-36 (1992)
  STANDARD  full automatic
COMMENT     *source: strain=BDF1; *source: cell_line=pre-B 702/3; *source:
            chromosome=16;
            From EMBL    entry MMBPBPRO;  dated 29-JUL-1992.
FEATURES             Location/Qualifiers
     promoter        1..817
     prim_transcript 657..>817
                     /gene="V-preB1"
BASE COUNT      236 a    184 c    210 g    187 t
ORIGIN
        1 ggatcctgtc ttaaaaaaaa aaaaaatata tatatatata tatgtatgta tatattcaga
       61 ttgtctcatt atgtagccaa ggccatcctg gaactcactg gaactcactg tggacccagg
      121 ctggcctcaa acttatgttt ttaagccatc cttttgttac caagactggc tatgacagcc
      181 ccaacttgtc aacgagacaa gtaagaaacc tataagatgg ctcagcaggc agacagtggc
      241 tgccaagctg gccatgtgaa cacaaaagaa atgaaaataa aaatgtaatt aaaagagata
      301 atactaaaaa gcagaggaag gagatttatt tagtgtgctc acatatggta gaggaacatc
      361 taaagaggcc cagtgactgc tcccacacat atttggggtc tgatatgagg cttaggttta
      421 aggagaaaac tcttgtcccc tttaccaggc atttaaagtg aactacagat ctgcttattt
      481 ggggctcagc ctctcaaggg gagttgaggt cactctccct cagggagaca agcccgcagt
      541 cctgggttct ggcccatgac gttccagcag gtgcttcctc ccagaatgct tccctgggtc
      601 aaacccagag ccacaaaggc ttccattaga ccattctggt aagtgacaga gtcacagagc
      661 ccagaaagcc tgggagggtg gtgagcagga accaggggtg cagtgaccct ctccccaaag
      721 cagggaggag agtgcttccc agctggtcag ggcccaggag cagtggctgt agggggcagg
      781 gtgctgcagg tctggagcca tggcctggac gtctgtc
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Gallus gallus Ig unproductively rearranged L-chain gene V-J-region,
Date: 21 Aug 92 10:05:57 GMT
Distribution: bionet
Checksum: 50538     3

LOCUS       CHKIGLAZG     427 bp ds-DNA             VRT       21-AUG-1992
DEFINITION  Gallus gallus Ig unproductively rearranged L-chain gene V-J-region,
            partial cds.
ACCESSION   M96971
KEYWORDS    immunoglobulin light chain; joining region; pseudogene;
            variable region.
SOURCE      Gallus gallus (individual_isolate REV-T (CSV), strain SC line
            (Hyline International, Dallas Center, Iowa), sub_species
            domesticus) (library: TB1(ZD2)) day 12 embryo bursa DNA.
  ORGANISM  Gallus gallus
            Eukaryota; Animalia; Chordata; Vertebrata; Aves; Neornithes;
            Neognathae; Galliformes; Phasianidae.
REFERENCE   1  (bases 1 to 427)
  AUTHORS   Benatar,T., Tkalee,L. and Ratcliffe,M.J.
  TITLE     Stochastic rearrangement of immunoglobulin variable-region genes in
            chicken B-cell development
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 89, 7615-7619 (1992)
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     intron          <1..105
                     /gene="IgL"
     CDS             106..>427
                     /pseudo
                     /note="V-J-region"
                     /product="immunoglobulin light chain"
                     /gene="IgL"
                     /codon_start=1
     mat_peptide     123..>427
                     /pseudo
                     /product="immunoglobulin light chain"
                     /gene="IgL"
                     /codon_start=1
     misc_recomb     399..400
                     /note="VJ junction"
                     /gene="IgL"
BASE COUNT       80 a    142 c    117 g     88 t
ORIGIN
        1 gggactgtgg gcacggggct ctgtcccact gctgcgcggg cagggctgtg cgtgcggggc
       61 cgtcactgat tgccgttttc tcccctctct cctctccctc tccaggttcc ctggtgcagg
      121 cagcgctgac tcagccgtcc tcggtgtcag cgaacccggg aggaaccgtc aagatcacct
      181 gctccgggga tagcagctac tatggctggt accagcagaa ggcacctggc agtgcccctg
      241 tcactgtgat ctatgacaac accaacagac cctcgaacat cccttcacga ttctccggtt
      301 ccaaatccgg ctccacagcc acattaacca tcactggggt ccgagccgac gacaatgctg
      361 tctattactg tgcgagtaca gacagcagca gtactgctga tttggggccg ggacaaccct
      421 gaccgtc
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human chromosome 4 sequence-tagged site STS4-338
Date: 21 Aug 92 10:09:07 GMT
Distribution: bionet
Checksum: 47479     3

LOCUS       HUM4STS338    445 bp ds-DNA             PRI       21-AUG-1992
DEFINITION  Human chromosome 4 sequence-tagged site STS4-338
ACCESSION   L00914
KEYWORDS    STS; human chromosome 4; sequence tagged site.
SOURCE      Homo sapiens, clone C4-187 from Los Alamos National Laboratory
            chromosome 4 cosmid library, 2D1; vector SuperCos 1 (Stratagene)
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 445)
  AUTHORS   Goold,R.D., diSibio,G., Dugaiczyk,A., Smith,K.A., Xu,H.,
            Magrane,G., Lang,D.B., Cox,D.R., Masters,S.B. and Myers,R.M.
  TITLE     Sequence-Tagged Sites for Human Chromosome 4
  JOURNAL   Unpublished (1992) see COMMENT for author address
  STANDARD  full automatic
COMMENT     PCR components: 25 ng of human genomic DNA, 10 pmol of each
            oligonucleotide, 200 micro-M dNTPs, 0.25 U Taq  polymerase (Cetus)
            in 10 micro-l of 50 mM KCl-20 mM  Tris-HCl, pH 8.3 (at room temp),
            2.5 mM MgCl-2.  Initial denaturation at 94degC for 1.5 min, then 30
            cycles of  94degC for 15 sec, 62degC for 23 sec, and 72degC for 30
            sec, followed by a final extension at 72degC for 3.5 min, using a
            Perkin-Elmer 9600 thermocycler. PCR-amplified product size 223 bp.
            Sequence submitted by:
            Human Genome Mapping Center
            Box 0925
            University of California San Francisco
            San Francisco, CA 94143-0925  USA
            Phone: (415) 502-1612   Fax: (415) 476-8391
            e-mail: hgmcprobes@cgl.ucsf.edu
FEATURES             Location/Qualifiers
     misc_feature    24..49
                     /label=Primer1
     misc_feature    complement(221..246)
                     /label=Primer2
BASE COUNT      139 a     73 c     97 g    128 t      8 others
ORIGIN
        1 tccaaaaagc aggtatttat aaagagagtt atttatctgg gtaaactcac taaaatttta
       61 gtgagtattt gggtgctcag attttaaaat caggcaagaa ttatnccagn tgaatggtgt
      121 ttaaatagaa taagacttat tattttctgg gtgtgtttta cctaatcctg gaatgcatat
      181 gataagatag gctgtagnct acagttttng gacctaaaat cattcagact ttaacacaat
      241 gacgttgcct ttccattctt gatgaacatc cgttaaattc agagtcacag aacaagatan
      301 tatcagaaag aagtggccgg gtgcggtggc tcacgcctgt aattccagca ctttgggagg
      361 ncgaggtgag tggatcatga ggtcaggaga tcgagtccat cctgggtaac atngcaaagg
      421 ccctnctcta ctaaaaatat aaaac
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Grifola frondosa ribosomal RNA (7LR0R) large subunit, partial.
Date: 18 Aug 92 10:00:56 GMT
Distribution: bionet
Checksum: 01161     2

LOCUS       GRIRRLSB      231 bp ss-rRNA            RNA       18-AUG-1992
DEFINITION  Grifola frondosa ribosomal RNA (7LR0R) large subunit, partial.
ACCESSION   M98641
KEYWORDS    large subunit ribosomal RNA.
SOURCE      Grifola frondosa (library: Duke Herbarium SAR 89-478) cDNA to rRNA.
  ORGANISM  Grifola frondosa
            Eukaryota; Fungi; Amastigomycota; Basidiomycetes.
REFERENCE   1  (bases 1 to 231)
  AUTHORS   Hibbett,D.S. and Vilgalys,R.
  TITLE     Phylogenetic relationships of the basidiomycete genus Lentinus
            inferred from molecular and morphological characters
  JOURNAL   Unpublished (1992)
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     rRNA            <1..>231
                     /note="Approximately aligns to region between positions 70
                     to 306in Saccharomyces cerevisiae large subunit nuclear
                     rRNA."
                     /product="large subunit ribosomal RNA"
                     /citation=[1]
BASE COUNT       53 a     49 c     71 g     58 t
ORIGIN
        1 tcccctagta actgcgagtg aagcgggaag agctcaaatt taaaatctgg cggtctcagg
       61 ccgtccgagt tgtagtctgg agaagtgctt tccgcgctgg accgtgtaca agtctcttgg
      121 aacagagcgt catagagggt gagaatcccg tctttgacac ggactaccag tgctttgtga
      181 tgcgctctcg aagagtcgag ttgtttggga atgcagctca aaatgggtgg t
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human chromosome 4 sequence-tagged site STS4-151
Date: 21 Aug 92 10:07:00 GMT
Distribution: bionet
Checksum: 00282     3

LOCUS       HUM4STS151    434 bp ds-DNA             PRI       21-AUG-1992
DEFINITION  Human chromosome 4 sequence-tagged site STS4-151
ACCESSION   M95265
KEYWORDS    STS; human chromosome 4; sequence tagged site.
SOURCE      Homo sapiens, clone C4-180 from Los Alamos National Laboratory
            chromosome 4 cosmid library, plate 4-1-2R, pos6C; vector SuperCos 1
            (Stratagene)
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 434)
  AUTHORS   Goold,R.D., diSibio,G., Dugaiczyk,A., Smith,K.A., Xu,H.,
            Magrane,G., Lang,D.B., Cox,D.R., Masters,S.B. and Myers,R.M.
  TITLE     Sequence-Tagged Sites for Human Chromosome 4
  JOURNAL   Unpublished (1992) see COMMENT for author address
  STANDARD  full automatic
COMMENT     PCR components: 25 ng of human genomic DNA, 10 pmol of each
            oligonucleotide, 200 micro-M dNTPs, 0.25 U Taq  polymerase (Cetus)
            in 10 micro-l of 50 mM KCl-20 mM  Tris-HCl, pH 8.3 (at room temp),
            2.5 mM MgCl-2.  Initial denaturation at 94degC for 1.5 min, then 30
            cycles of  94degC for 15 sec, 62degC for 23 sec, and 72degC for 30
            sec, followed by a final extension at 72degC for 3.5 min, using a
            Perkin-Elmer 9600 thermocycler. PCR-amplified product size 192 bp.
            Sequence submitted by:
            Human Genome Mapping Center
            Box 0925
            University of California San Francisco
            San Francisco, CA 94143-0925  USA
            Phone: (415) 502-1612   Fax: (415) 476-8391
            e-mail: hgmcprobes@cgl.ucsf.edu
FEATURES             Location/Qualifiers
     misc_feature    17..41
                     /label=Primer1
     misc_feature    complement(184..208)
                     /label=Primer2
BASE COUNT      146 a     59 c     91 g    108 t     30 others
ORIGIN
        1 aaaagtagcc cccaaacagg agcaacaaca acaaaaaact gttgaatgat tagtttaatt
       61 caagtgggaa ataataattg tgtcagagga gaagagaaga aagattcaag agataccctg
      121 gatgtagtgt ttacaagatt tagtaatcat cgaatataag gtagcacaaa gtagatgtgc
      181 gtaaactcat ggctctgggt gaaactatnt ggcttttatt ctnttaacaa agacagaata
      241 attcgngtag ttgnaatgcc agtgaacatt tagaantaaa tagccagnag ccanttanaa
      301 agatanatat aganctnaag aggaactttg nctgagagat acagatttgg gtncccncag
      361 cttncaaact nnangctgat ngtattgtcg cagattnggn ttnttanngg agagcgcntn
      421 nctnttntgt gncc
//