From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Spinacia oleracea 10-formyltetrahydrofolate synthetase (sfs1) mRNA,
Date: 4 Sep 92 10:07:09 GMT
Distribution: bionet
Checksum: 59827     5

LOCUS       SPISFS1A     2221 bp ss-mRNA            PLN       04-SEP-1992
DEFINITION  Spinacia oleracea 10-formyltetrahydrofolate synthetase (sfs1) mRNA,
            complete cds.
ACCESSION   M83940
KEYWORDS    10-formyltetrahydrofolate synthetase; cytoplasm;
            monofuntional protein.
SOURCE      Spinacia oleracea leaf cDNA to mRNA.
  ORGANISM  Spinacia oleracea
            Eukaryota; Plantae; Embryobionta; Magnoliophyta; Magnoliopsida;
            Caryophyllidae; Caryophyllales; Chenopodiaceae.
REFERENCE   1  (bases 1 to 2221)
  AUTHORS   Nour,J.M. and Rabinowitz,J.C.
  TITLE     Isolation and sequencing of the DNA coding for spinach
            10-formyltertrahydrofolate synthetase: Comparisons with the yeast,
            mammalian, and bacterial proteins
  JOURNAL   J. Biol. Chem. 267, 16292-16296 (1992)
  STANDARD  simple staff_entry
FEATURES             Location/Qualifiers
     CDS             28..1941
                     /EC_number="6.3.4.3"
                     /product="10-formyltetrahydrofolate synthetase"
                     /gene="sfs1"
                     /codon_start=1
                     /translation="MGGSSKTVRKLEVATPVPADIDIANAVEPLHIADIAADLNLSPR
                     HYDLYGKYKAKVLLSVLDEVQETQDGYYVVVGGITPTPLGEGKSTTTVGLCQALGAFL
                     DKKVVTCLRQPSQGPTFGIKGGAAGGGYSQVIPMDEFNLHLTGDIHAITASNNLLAAA
                     IDTRMFHESTQSDKALFNRLCPPNKEGKRTFCNIMHRRLKKLGIDKTNPDDLTPEEVT
                     KFARLDIDPDSITWRRVMDVNDRFLRKISVGQGPDEKGMVRETGFDISVASEIMAVLA
                     LTTSLADMRERLGKMVIGNSKAGEPITADDLGLGGALTVLMKDAINPTLMQTLEGTPV
                     LVHAGPFANIAHGNSSIVADKIALKLVGPGGFVVTEAGFGSDIGTEKFMNIKCRYSGL
                     TPQCAIVVATVRALKMHGGGPQVVAGKPLDRAYLTENVGLVEAGCVNLARHIINTKAY
                     GSNVVVAINMFSSDTEAELNAVKKAAMDAGAFDAVICTHHAHGGKGAVDLGIAVQKAC
                     ENVTQPLRFLYPLDISIKEKIEAIAKSYGAAGVEYSEQAEKKIEMYSKQGFSNLPICM
                     AKTQYSFSHNAAEKGAPSGFILPIRDVRGSIGAGFIYPLVGTMSTMPGLPTRPCFFDI
                     DLDTTTGKVIGLS"
BASE COUNT      613 a    426 c    542 g    640 t
ORIGIN
        1 cggcacgaga ttggattaac caaaaaaatg ggaggttcat cgaaaacggt gaggaagtta
       61 gaggtggcga caccggtacc ggcggacatt gacatagcaa atgcggtgga gccgcttcat
      121 atcgctgata ttgccgccga tctcaatctc tctcctcgtc actacgacct ttatggcaaa
      181 tacaaggcca aggttttgct gtctgtgttg gatgaagttc aagaaaccca ggatggatat
      241 tacgtcgtgg ttggtggaat tactcctact cctctcgggg aaggcaaatc taccaccact
      301 gttggtcttt gtcaagcctt gggagctttc cttgataaga aggttgtcac ttgccttcgt
      361 caaccatcac aagggccaac ttttggaata aaaggtggtg cagctggtgg tggttatagt
      421 caagtgattc caatggatga gttcaacctt cacttaactg gggatataca tgctataaca
      481 gcttcaaaca atcttctcgc tgctgccata gacaccagaa tgttccatga gtccacccag
      541 tcagataaag ctctctttaa ccgtttgtgc ccccctaaca aagaaggtaa aagaaccttt
      601 tgtaacatta tgcacaggcg tttgaaaaag cttggtattg ataagactaa tccagacgac
      661 cttacaccgg aagaggtcac aaaatttgct aggcttgata ttgaccccga ttccattaca
      721 tggagaagag ttatggatgt gaatgaccgg tttttgagga aaattagtgt tggtcagggg
      781 cctgatgaga aggggatggt aagagaaact ggatttgata tttccgttgc tagtgagata
      841 atggcagttc tggctctaac aacttctctg gctgatatga gggagagact tgggaaaatg
      901 gtgattggaa atagcaaggc tggagaaccg attactgctg atgatcttgg cttaggtggt
      961 gctttgactg tattaatgaa ggatgcaatc aaccccacgc tgatgcagac tctggaagga
     1021 acacctgtgc ttgttcatgc tggtcctttt gcaaatattg ctcatggtaa ttcctctatt
     1081 gttgctgaca agattgccct gaagctggtt ggacctggtg ggtttgtggt tactgaagca
     1141 gggtttggtt ctgatatcgg aactgagaag ttcatgaaca taaagtgccg atatagtggt
     1201 ttgactccac agtgtgctat tgttgttgcg acagtgaggg ctttgaaaat gcatggtggt
     1261 ggaccacaag tagtggctgg caagcctctt gatcgtgcat acttaacaga gaatgtgggt
     1321 cttgttgagg ctggctgtgt taatcttgcc aggcatatta taaatacgaa ggcctatggt
     1381 tcaaatgtcg tggttgccat caacatgttt tcttctgaca cagaagcaga gctgaatgct
     1441 gttaaaaaag cagcaatgga tgctggagcg tttgacgcag ttatttgtac ccatcacgcc
     1501 catggtggaa aaggagcggt ggatcttggc atagctgttc agaaagcatg tgagaacgta
     1561 acgcagcctt tgaggttctt atatcctctg gatattagca tcaaagagaa aatagaggca
     1621 attgcaaagt cttatggtgc tgctggcgtc gaatactcag aacaggctga gaaaaagatt
     1681 gagatgtaca gcaagcaagg gttctccaat cttccaatat gcatggcaaa aacgcaatat
     1741 tcattttccc acaatgctgc tgaaaaggga gctccaagcg gatttatctt gcctattcgg
     1801 gatgttagag gtagcatcgg agctggattc atttacccgt tggtaggaac catgagtaca
     1861 atgcctgggc tcccaacaag gccgtgcttc tttgatattg atctcgacac tacaacaggg
     1921 aaggttatcg gtctttctta ggaaaaaaat tgagttagtt tcccttttcc agccaaactt
     1981 cttggacctt ttttattttt tgtatgaact tctgtatttc ttatgagcca ttatttccca
     2041 ttgcttcact aataaataga agctaataag tcttgccctt tgttcggtct gtgtacccct
     2101 cggcctttct atactagatg tgtgcatcaa tgttggatct tatttgtaat catcaaattt
     2161 atagattata caagtaatct gaagatggtt attttaccat caaaaaaaaa aaaaaaaaaa
     2221 a
//