HDTenEightyP/NetNews huggingface dataset:
From: CRHUGHES@VM1.NODAK.EDU (Colin Hughes)
Newsgroups: bionet.molbio.gdb
Subject: Microsatellites
Date: 1 Oct 92 19:58:09 GMT
Distribution: bionet
Organization: North Dakota Higher Education Computer Network


  I am looking for a compilation of data on human microsatellite loci.  Ideally
I would like to find a source that has the following information on all such
loci that have been studied:  sequence, number and frequency of alleles, size
of alleles in bp, the heterozygosity or PIC value and the number of genomes
screened.  Does such a thing exist?  Can I get the info without paying an
enormous sum?  thanks  Colin.
From: chipper@welchgate.welch.jhu.edu (Mike Chipperfield)
Newsgroups: bionet.molbio.gdb
Subject: Re: Microsatellites
Date: 6 Oct 92 20:37:19 GMT
Distribution: bionet
Organization: Johns Hopkins Univ. Welch Medical Library

In article <1992Oct2.151057.1@kean.ucs.mun.ca> roger@kean.ucs.mun.ca writes:
>In article <1992Oct2.145619.24655@welchgate.welch.jhu.edu>, chipper@welchgate.welch.jhu.edu (Mike Chipperfield) writes:
>> In article <9210012005.AA05209@net.bio.net> CRHUGHES@VM1.NODAK.EDU (Colin Hughes) writes:
>>>
>>>  I am looking for a compilation of data on human microsatellite loci.  Ideally
>>>I would like to find a source that has the following information on all such
>>>loci that have been studied:  sequence, number and frequency of alleles, size
>>>of alleles in bp, the heterozygosity or PIC value and the number of genomes
>>>screened.  Does such a thing exist?  Can I get the info without paying an
>>>enormous sum?  thanks  Colin.
>> 
>> 
>> Colin,
>> 
>> Later on this fall, the latest edition of Genome Maps (edited by Stephen J.
>> O'Brien, Frederick Cancer Research Institute, Frederick, MD) will be published.
>> This will have a chapter containing data on all PCR-detected polymorphisms (the
>> vast majority of which are microsatellites) stored in GDB, as of Sept. 1, 1992.
>> The data will include primer sequences, max heterozygosity, number of alleles,
>> cytogenetic location, locus symbol and locus name.
>> 
>> If you need further information regarding this data, please contact me
>> directly. My email address is: chipper@welchgate.welch.jhu.edu
>> 
>> Sincerely,
>> 
>> Michael A. Chipperfield
>> DNA Data Coordinator
>> Genome Data Base
>		----------------------------------------------
>
>	A lot of people will be looking for this type of information on a 
>regular basis.  Will GDB consider putting together such a database and 
>making it obtainable via FTP ?  Monthly updates would be ideal!
>
>
>Roger C. Green,	Faculty of Medicine                      Phone: (709)737-6884
>Memorial University , St. John's, Newfoundland.          FAX  : (709)737-7010


GDB is currently in the process of releasing a new version, GDB version 5.0.
One of the features being planned is a set of standard reports of interest
to the community, to be placed on our anonymous FTP server. This report will
be made available at that time.

Our FTP server currently includes a number of items of interest:

	- Weekly statistics of GDB
	- GDB schema and data dictionary
	- Forms for data submission to GDB

The name of our server is mendel.welch.jhu.edu (128.220.59.42). Feel free to
explore the items on this server, and let us know if there are any additional
items which may be of interest that should be included.

Sincerely,

Michael A. Chipperfield
DNA Data Coordiantor
Genome Data Base
From: s882076@yallara.cs.rmit.OZ.AU (Sean Davidson)
Newsgroups: bionet.molbio.methds-reagnts
Subject: Re: LUCIFERASE EXPRESSION
Date: 30 Sep 92 13:34:10 GMT
Distribution: bionet

endo@angis.su.OZ.AU (Prince of Wales Endocrinology Unit) writes:


>Hi I'm using luciferase as a reporter gene in primary cultures
>of rat Leydig cells.  I posted a message previously asking if
>anyone has encountered the problem that I'm having.  I know
>that my cells are successfully transfected (the mRNA is present)
>and the extraction and assay are working well in other cells yet
>I get no detectible activity in my Leydig cell extracts.  

Sorry, I don't have any hints - just a further question:
I am hoping to get a luciferase reporter gene soon, and would like
to know how to assay it! I know of the method using a luminometer,
which I do not have, but I understand a scintillation counter can
be rigged up to work in a similar manner. Can some-one tell me
how this is done and how well it works?
Thanks, Sean
Newsgroups: bionet.molbio.methds-reagnts
From: gsouth@genome.wi.edu (Genome South user)
Subject: Hot Start PCR
Organization: Whitehead Institute for Biomedical Research
Distribution: bionet
Date: Fri, 2 Oct 1992 12:20:42 GMT

"Hot-start" PCR is a method that generally produces cleaner PCR
products.  Take your template DNA, denature it, and, leaving it
well above the annealing temp of your primers, add your buffer,
dNTPs, primers, and Taq.  The results tend to be cleaner because
the primers don't have a chance to anneal non-specifically at
a lower temperature.

This method is really a pain to do (you pretty much have to use
a 100C heat block as your work surface), so I do what I call
"hemi-demi-semi Hot-start";  denature your template DNA, snap
cool on ice, and LEAVE it on ice while you add the PCR mix
(primers, buffer, dNTPs, Taq).  Then put it on the PCR machine
(excuse me _thermocycler_) which is _already)_ at the denaturing
temperature.  This means that the primers never get a chance
to anneal at the wrong temperature.

Joyce
jcmill@eagle.mit.edu
From: jjw@rsbs0.anu.edu.au ( Jeremy Weinman)
Newsgroups: bionet.molbio.methds-reagnts
Subject: PCR to prove transgenic plants
Date: 6 Oct 92 05:29:15 GMT
Organization: Research School of Biological Sciences, Australian National University

Hello fellow netters,
I'm after any info/hints/protocols/suggestions you might have regarding the 
PCR detection of transgenic plants.  We've used a binary Ti system using 
A. tumafaciens to (presumably) make the transgenic plants, but as they're 
onle small little plantlets so far, I was considering a PCR approach to 
testing for the presence of the intoduced construction. (Only 1 leaf 
needed).
Please send any ideas or experiences you've had with such methods to me and
I'll summarise to the group.
Thanxs in advance,
		Cheers,
			Jeremy
=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=
Dr. Jeremy Weinman				Email:	jjw@rsbs1.anu.edu.au	
Plant Microbe Interaction Group			Phone:	61 6 2495051
Research School of Biological Sciences		Fax:	61 6 2490754
Australian National University			Snail:	PO Box 475, Canberra, 
							ACT 2601 AUSTRALIA
From: rhughes@UCSD (Richard J. Hughes)
Newsgroups: bionet.molbio.methds-reagnts
Subject: Primer compilation
Date: 28 Sep 92 17:26:40 GMT
Organization: ucsd.edu
NNTP-Posting-Host: sombsb224.ucsd.edu

I have a copy of the source code for Lander's primer but I'm not a 
programmer and I don't have a C compiler.  So far, at least, I haven't been 
able to find anyone else who has either.

So...could someone please mail me a copy of the executable files or post 
these on an ftp site?  Why aren't the executables posted along with the 
source code?

Thanks.

Richard J. Hughes
rhughes@ucsd.edu
From: gilbertd@sunflower.bio.indiana.edu (Don Gilbert)
Newsgroups: bionet.molbio.methds-reagnts
Subject: Re: Umea Drosophila stocks
Date: 2 Oct 92 22:33:37 GMT
Organization: Biology, Indiana University - Bloomington

In article <1992Sep28.213506.48246@embl-heidelberg.de> rice@embl-heidelberg.de (Peter Rice) writes:
>I have the Drosophila stock listings from the Indiana FTP site, but our
>fly labs here would like the listing from the European stock centre
>at Umea in Sweden. Something to do with how long it takes a fly to fly
>from the USA I suspect.
>
>Is the Umea list available online anywhere?

Thanks to the hard work of Kathy Matthews, curator of Bloomington's
Drosophila stock center, the Umea fly stock list is now available
electronically.  You can fetch it by ftp from ftp.bio.indiana.edu
as /fly/Umea.txt (a spreadsheet format), as /fly/gopher-s/umeagoph.txt
(a somewhat more readable format), or you can use Internet Gopher
to ftp.bio.indiana.edu, and ask questions of the
"Drosophila/Drosophila Stocks?" item  which will list out fly stocks
matching key words from three stock centers, Umea, Bloomington
and Bowling Green.   There will be new gopher items soon to let
you search individual stock center lists.

Now that an electronic list of the Umea stocks is available, Dr. Matthews
would prefer that orders for Drosophila stocks from Europe first go
to the Umea center if they have the stock.

The flies probably would prefer not to fly the Atlantic also.

-- Don
-- 
Don Gilbert                                     gilbert@bio.indiana.edu
biocomputing office, biology dept., indiana univ., bloomington, in 47405
From: rhughes@UCSD (Richard J. Hughes)
Newsgroups: bionet.molbio.methds-reagnts
Subject: Brain cDNA library
Date: 6 Oct 1992 23:42:01 GMT
Organization: ucsd.edu
NNTP-Posting-Host: sombsb224.ucsd.edu

	Quite recently , I started to clone certain receptor using Xenops 
oocyte expression system. However, my initial trial failed. Since the 
positive control (purified mRNA of other receptor) works fine, I think the 
library I used does not contain enough message (or does contain only 
partial / broken message) for the target protein.
	Is there someone who has rat (or dog) brain cDNA liblary in 
lambda-ZAP phage vector ? I would appreciate it very much if I could contact 
someone who has such a library.

Kazushi Urasawa
Dept. of Pharmacology, UCSD
KURASAWA@UCSD.EDU
From: kim@m44.unm.edu
Newsgroups: bionet.molbio.methds-reagnts
Subject: Re: Hot Start PCR
Date: 2 Oct 92 21:58:10 GMT
Reply-To: kim@m44.unm.edu
Distribution: bionet
Organization: UNM Cancer Center, Albuquerque, NM

In article <1992Oct2.122042.15605@genome.wi.edu>, gsouth@genome.wi.edu (Genome South user) writes:

[Stuff on hot start pcr deleted]

>This method is really a pain to do (you pretty much have to use
>a 100C heat block as your work surface), so I do what I call
>"hemi-demi-semi Hot-start";  denature your template DNA, snap
>cool on ice, and LEAVE it on ice while you add the PCR mix
>(primers, buffer, dNTPs, Taq).  Then put it on the PCR machine
>(excuse me _thermocycler_) which is _already)_ at the denaturing
>temperature.  This means that the primers never get a chance
>to anneal at the wrong temperature.

Hot starts are also done by creating a physical barrier between the primers and
template.  This barrier is created by putting a half-reaction mixture (buffer
and primers) into the bottom of the tube and melting wax over the mix.  The wax
used can be "PCR Gems" from Perkin-Elmer/Cetus or any number of home-grown
waxes described in this group (e.g. paraffin or Paraplast).  After the wax
barrier hardens, put the other half-reaction on top (template, buffer, and
enzyme).  Put the tube into the thermocycler and run.  As the machine brings
the mix up to denaturation temperature, the wax barrier melts, allowing the two
half reactions to mix only when the temperature has gone above the melting
temperature of the wax.  From this point on the PCR cycles proceed normally.

This obviates the need to pre-denature the DNA and watch over the reactions. 
In addition, the wax barrier hardens after the cycles are complete and the
temperature of the tube goes to room temp or 4 C.  It is fairly to recover the
sample from beneath the barrier with a minimum of contaminating wax.

Perkin-Elmer/Cetus would probably be happy to send literature describing the
Hot Start technique as it pertains to PCR Gems.  Several posters (?) described
successful use of alternative waxes to the gems, so there doesn't seem to be
any mystery about them.

Daniel Kim
KIM@FLOVAX.LANL.GOV
From: jmasters@magnus.acs.ohio-state.edu (Jeffrey N Masters)
Subject: Re: Etd-Br Mapping
Organization: The Ohio State University
Distribution: bionet
Date: Tue, 6 Oct 1992 13:54:12 GMT

In article <9210021714.AA05754@net.bio.net> KNECHT@UCONNVM.UCONN.EDU writes:
>Hi Netters-  I remember reading somewhere that you can use ethidium bromide in
>restriction digestions to make partial digests.  Does anyone know a protocol o
r
> reference to this technique.  Thanks- Dave Knecht (KNecht@uconnvm.edu)

Parker et al., PNAS 74:851-855 1977
Newsgroups: bionet.molbio.methds-reagnts
From: sknapp@helix.OSCS.ORST.EDU (Steven J. Knapp)
Subject: 33p
Reply-To: sknapp@helix.OSCS.ORST.EDU
Organization: University Computing Services - Oregon State University
Date: Mon, 28 Sep 1992 20:03:21 GMT

Sorry, but I missed the replys (if any) on the use/recommendations on using  
this isotope for Southerns.  Altho I've switched to chemiluminescence, there is  
occasionally a need for using isotope and I'd like to get away from 32p if  
possible.  Any info would be helpful.  Thanks.
From: wetsel_r@wums.wustl.edu
Newsgroups: bionet.molbio.methds-reagnts
Subject: RE: hybridization problems
Date: 5 OCT 92 10:17:57 GMT
Organization: MIT PLASMA FUSION CENTER
NNTP-Posting-Host: msnews.wustl.edu

In a previous article, Klaus.Matthaei@anu.edu.au wrote:
>>To molecular biologists:
>>I am having a problem in probing Northern blot membranes ans slot blot 
>>membranes (Hybond N+). Sllight hazy background is evident on the 
>>membranes, suggesting that the probe is OK.  However, where the RNA is 
>>applied in slot blots or where it has migrated to in Northerns appears 
>>white.  The image looks like a negative.
>>	If you have encountered this problem or if you know of a 
>>possible solution, please leave a message here.
>>Thanks. 
>>Juan
> 
> 
>G'Day Juan
> 
>You have a common problem of not blocking your membrane against
>non-specific hybridisation.  Nylon membrane needs better blocking than
>nitrocellulose.  The negative image is due to the probe not hybridising to
>that part of the membrane and because you have no target = no signal.
> 
>For a homologous DNA probe try hybing in: 50% deionised formamide, 2 x PE,
>7% SDS, 1% BSA (0.5mg/ml Salmon Sperm DNA optional) at 50 degrees.  Wash
>down to 0.2x SSC 1% SDS at 70.
> 
>Good Luck..Klaus
> 
>--------------------------------------------------------------------------
>Catherine Shang
>Gene Targeting
>The John Curtin School of Medical Research
>The Australian National University
>E-mail: Catherine.Shang@anu.edu.au
> 
> 
>--------------------------------------------------------------------------

Greetings... Normally I would agree with Catherine.  However, my wife
and I have experienced this problem in our lab too and it is her impression
that the problem lies with the lot of N+.  We have always used the standard
hybridization solution (5X SSC,, 0.2%Ficoll 400,0.2% BSA, 0.2%PVP,0.5%pyro,
and 1% SDS) ala Maniatis (Salmon sperm optional) and this has worked fine
for us for years...  until we got this one lot of N+ (#13709) and then all
h*ll broke loose.  As initially described, our bands were shadowed and the
background on the blot high.  Only when my wife changed lots of N+ and repeated
the Southern did it work with low background.  

Catherine's advise is sound; however, have you had this problem before with
different lots of N+ or is this a new problem?

Thanks,

David
haviland@kids.wustl.edu
From: bsh@MED.PITT.EDU (Basavaraju Shankarappa)
Newsgroups: bionet.molbio.methds-reagnts
Subject: Re: immune precipitation
Date: 5 Oct 92 13:42:11 GMT
Distribution: bionet

> In a previous article, skspoidn@susssys1.reading.ac.uk () says:
> >I am trying to Immune precipitate a cellular protein 
> >(CD4) with Mabs using protein A and S35 metabolically 
> >labelled cells, and finding that the immune reaction is
> > not occuring, although antibodies are being precipitated.
> > Is there some aspect of the immune reaction and susequent
> > precipitation which is critical in this procedure?
> >
> to PrA, I'd cite the classes here but I'd probably mix them up.  We usually
> test a new mAb empirically by binding some to PrASepharose and eluting and
> staining for the IgG on a PAGE.
> 
> If your mAb doesn't bind to PrA directly, not all is lost, as you can add
> rabbit anti-mouse IgG to sandwich the mAb to the PrA with the rabbit IgG
> that binds pretty efficiently.  In my experience, the Rabbit anti-mouse
> serum doesn't affect the quality of the IP's very much.
> dennis
	One suggestion to overcome these problems.  I had used this procedure
with some success dut did not need to explore in detail.  
Can we not use the say anti-mouse immunoglobulins that are bound to a solid-
phase.  Since these are polyclonal, I think it should be fairly easy for them
to bind as opposed to the limited binding epitope of ProteinA.  I know two
suppliers one is Kirkegaard and Perry Lab at Gaithersburg Md and the other is
Pierce (Call 1-800-555-1212) for both Toll free numbers.  
	If somebody has tried this method, can you please add your comments?
Raj Shankarappa
Univ of Pittsburgh.
From: jasper@vax.path.ox.ac.uk
Newsgroups: bionet.molbio.methds-reagnts
Subject: Re: ***Help CHICKEN cell line (urgent)******
Date: 6 Oct 92 19:16:03 GMT
Organization: Oxford University Molecular Biology Data Centre
Nntp-Posting-User: jasper


> 
> I am looking for an easy-to-grow, easy-to-maintain and easy-to-transfect
> CHICKEN cell line. Just about any type of chicken cell line will meet our needs as long as it can be grown and transfected with relative ease. 
> If anybody has such a chicken cell line I would appreciate hearing from you.
> Any response is welcome. Thanks.
> 

The only lines that grow in culture other than primary ones are Marek's
Lymphoma derived, there are (to the best of my knowledge) no others. I'm
afraid I don't have a contact for these, though if pushed I *might* be able to
contact a vetinarian who knows unless he has already gone to the Himilayas for
6 weeks vacation ! 

Anyone knowing of any others??? 

> 
> Hirdeypal Bhathal
> Dept. of Chemistry
> SDSU, San Diego 
> CA-92182, USA
> (619)594-3771
> hsb@ucselx.sdsu.edu
> hsb@chem.ucsd.edu

regards,    jasper


Jasper Rees
The Sir William Dunn School of Pathology
Oxford University

jasper@vax.path.ox.ac.uk
Newsgroups: bionet.molbio.methds-reagnts
From: don@helix.nih.gov (donald seto)
Subject: Re: Photographing Coomasie-stained Gels
Organization: National Institutes of Health, Bethesda
Date: Thu, 1 Oct 1992 17:47:52 GMT

In article <1992Sep29.165234.1@mscf.med.upenn.edu> kaya@mscf.med.upenn.edu writes:
>I photograph with a light box and prefer a yellow filter for Coomassie Blue 
>stained gels.
>
kodak sells a 1:1 contact photographic system, where all you need is
a lamp plus some dev solns.  good for coomassie and silver stains
also for westerns.
From: KNECHT@UCONNVM.UCONN.EDU (Dave Knecht)
Newsgroups: bionet.molbio.methds-reagnts
Subject: Hybridization Incubators
Date: 27 Sep 92 04:44:33 GMT
Distribution: bionet

I have been considering buying one of the new style rotating cylinder hybridiza
tion incubator for Southerns etc.  The look like a significant improvement over
 traditional methods.  However, I have never actually used one or know anyone t
hat has.  Any comments from Netland about various models or general utility of
these units.  The Techne style rotating cylinder looked better than the Bellco
style, but I would love to hear from some actual users.


Dave Knecht
Knecht@Uconnvm.edu                    "A foolish consistency is the hobgoblin
                                       of little minds"
Newsgroups: bionet.molbio.methds-reagnts
From: broe@aardvark.ucs.uoknor.edu (Bruce Roe)
Subject: Re: Size of exons and introns?
Originator: news@midway.ecn.uoknor.edu
Date: Sat, 3 Oct 1992 12:47:00 GMT
News-Software: VAX/VMS VNEWS 1.41    
Organization: University of Oklahoma - University Computing Services

In article <robison1.717956732@husc10>, robison1@husc10.harvard.edu (Keith Robison) writes...
>naffara@crc.ac.uk (Dr. N.A. Affara) writes:
> 
>>Does anyone know the maximum, minimum, (if any) and average size of exons
>>and introns in human DNA?
> 
>	I posted something a while back on the subject -- you might try
>the archives.  The largest exon known is in apolipoprotein B and is
>over 7 Kb.
>	If memory serves me, human introns smaller than 75 bp are
>very rare, but the largest ones can be enormous (10s of Kb, probably
>some in the 100s of Kb).
> 
>Keith Robison
>robison@ribo.harvard.edu 

The human cAbl gene has a 175kb first intron and I'm sure there are
other's out there even bigger.

Keith, it was nice to finally put a face with a name.

Cheers..........bruce
		broe@aardvark.ucs.uoknor.edu
From: pnh@fcsparc6.ncifcrf.gov (Paul N Hengen)
Newsgroups: bionet.molbio.methds-reagnts
Subject: Re: MICROPIPETTE CALIBRATION (FAQ contributions)
Summary: FAQ list creation
Date: 29 Sep 92 15:30:26 GMT
Distribution: bionet
Organization: Frederick Cancer Research and Development Center


WAGNERF@BRUNB.BITNET (Wagner Fontes) writes:

:Thanks for everyone who wrote an answer to my problem.
:Since I started the discussion, I'm making it's summary.

What a great idea! May I suggest others do this as well?
I'm saving a bunch of these things to create a FAQ list
for this newsgroup. If anyone has a list of questions or
has edited answers from other questions, you can e-mail
them to me and I'll put them together. Now that this group
may grow from the recent non-electronic publicity, we may
get swamped with the typical new-user questions...

Any other suggestions are welcome.

Paul N. Hengen
National Cancer Institute
Frederick Cancer Research and Development Center
Frederick, Maryland 21702-1201 USA
e-mail: pnh@ncifcrf.gov
Newsgroups: bionet.molbio.methds-reagnts
From: mpragnell@vaxa.weeg.uiowa.edu (Marlon Pragnell)
Subject: Looking for a good oligo design program. Help!
News-Software: VAX/VMS VNEWS 1.41    
Organization: University of Iowa, Iowa City, IA, USA
Date: Sun, 27 Sep 1992 03:05:00 GMT

Hi! our lab is looking for a good oligo design program
to help in choosing oligos for PCR and sequencing.  Does 
anyone have any suggestions?  Thanks!

Marlon Pragnell
mpragnell@vaxa.weeg.uiowa.edu
From: acc4636@tamsun.tamu.edu (Anthony C.)
Newsgroups: bionet.molbio.methds-reagnts
Subject: Re: Cetus questions.
Date: 3 Oct 92 03:50:54 GMT
Organization: Texas A&M University, College Station

The crowe article is the article.
From: inoue@ACS.UCALGARY.CA
Newsgroups: bionet.molbio.methds-reagnts
Subject: Genomic DNA purification system
Date: 30 Sep 92 18:23:41 GMT
Distribution: bionet


I am interested in using Invitrogen Genomic DNA Purification system, TurboGen and Micro-TurboGen. Can anyone using these systems provide opinions?

Masafumi Inoue
inoue@acs.ucalgary.ca
Molecular Pathology
U. of Calgary
Canada
From: pnh@fcsparc6.ncifcrf.gov (Paul N Hengen)
Newsgroups: bionet.molbio.methds-reagnts
Subject: Re: UV Shadowing
Date: 29 Sep 92 15:52:08 GMT
Organization: Frederick Cancer Research and Development Center

In article <19ss5jINN2dh@usenet.INS.CWRU.Edu>
djt2@po.CWRU.Edu (Dennis J. Templeton) writes:

:>>Hi!  To UV shadow an oligonucleotide, we pick the gel up on an intensifying
:>>screen - like you would get out of a film casette.  Then, we shine a hand-held
:>>UV lamp on it in the dark.  To take a photograph, we just put the UV lamp down

:>I mentioned this once before, but it is worth repeating... Not ALL 
:>intensifying screens work for this application, some just barely glow. 
:>Specifically, none of our Dupont Cronex (bought 2 years ago) work. 
:>If yours does, set it aside for this purpose.
:>TLC plates "with fluorescence indicator" all seem to work though.

:>dennis

Some people also use plain white typing paper, but this is not
as sensitive. It was mentioned in B.R.L. Focus 9:3, pg.16 (1987)
and 10:3, pg.57 (1988).

Paul N. Hengen
National Cancer Institute
Frederick Cancer Research and Development Center
Frederick, Maryland 21702-1201 USA
From: kaya@mscf.med.upenn.edu
Newsgroups: bionet.molbio.methds-reagnts
Subject: RE: Cracked PAGE gels
Date: 29 Sep 92 21:45:37 GMT
Organization: Univ of Penn - Med School Computer Facility

I have used the following destain/presoak for acrylamide gels:

30% Methanol
10% glycerol

I cannot find the reference for this, but I have used it for gels up to 27% 
(only some cracking with 27% gels)- the longer I soak the better the results 
with high percentage gels.

I hope this helps!

angie.