From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Ostertagia ostertagi cathepsin B-like cysteine protease gene, 5'
Date: 7 Jan 93 05:52:50 GMT
Distribution: bionet
Checksum: 29571 8

LOCUS       OSTCATBCPA   2500 bp ds-DNA             INV       06-JAN-1993
DEFINITION  Ostertagia ostertagi cathepsin B-like cysteine protease gene, 5'
            end.
ACCESSION   M88503
KEYWORDS    cathepsin B-like cysteine protease.
SOURCE      Ostertagia ostertagi DNA.
  ORGANISM  Ostertagia ostertagi
            Eukaryota; Animalia; Eumetazoa; Nematoda; Secernentea; Rhabditia;
            Strongylida; Strongylina; Trichostrongyloidea; Trichostrongylidae.
REFERENCE   1  (bases 1 to 2500)
  AUTHORS   Pratt,D., Boisvenue,R.J. and Cox,G.N.
  TITLE     Isolation ofputative cysteine protease genes of Ostertagia
            ostertagi
  JOURNAL   Mol. Biochem. Parasitol. 56, 39-48 (1992)
  STANDARD  full automatic
COMMENT     This is the 5' end of the gene, it includes exons 1-9 and the first
            178 bp of intron 9. The 3' end of the gene, exons 10-12, is
            reported separately. Intron 9 (
            1.9kb) has not been sequenced.
FEATURES             Location/Qualifiers
     exon            1..206
                     /number=1
                     /partial
                     /note="putative"
     intron          207..282
                     /number=1
     exon            283..346
                     /number=2
     intron          347..407
                     /number=2
                     /note="alternative 3' splice site for Intron 2; putative"
     intron          347..419
                     /number=2
                     /note="putative"
     exon            408..502
                     /number=3
                     /note="alternative splice acceptor location for Exon 3.;
                     putative"
     exon            420..502
                     /number=3
     intron          503..558
                     /number=3
     exon            559..689
                     /number=4
     intron          690..1103
                     /number=4
     exon            1104..1171
                     /number=5
     intron          1172..1231
                     /number=5
     exon            1232..1305
                     /number=6
     intron          1306..1364
                     /number=6
     exon            1365..1417
                     /number=7
     intron          1418..2038
                     /number=7
     exon            2039..2120
                     /number=8
     intron          2121..2171
                     /number=8
     exon            2172..2322
                     /number=9
     intron          2323
                     /number=9
                     /partial
                     /note="intron 9 has not been sequenced"
     CDS             join(204..206,283..346,420..502,559..689,1104..1171,
                     1232..1305,1365..1417,2039..2120,2172..2322)
                     /partial
                     /note="putative"
                     /product="cathepsin B-like cysteine protease"
                     /codon_start=1
                     /translation="MKYLFFALCLYLYQGISEAEVPAEQIPLEAQALSGLPLVEYLQK
                     NQRLFEVTATPVPYFKQRLMDLKYIDQNNIPDEEVEDEELEENNDDIPESYDPRIQWA
                     NCSSLFHIPDQANCGSCWAVSSAAAMSDRICIASKGAKQVLISAQDVVSCCTWCGDGC
                     EGGWPISAFRFHADEGVVTGGDYNTKGSCRPYEIHPCGHHGNETYYGECVGMADTPRC
                     KRRCLLGYPKSYPSDRYY"
     CDS             join(204..206,283..346,408..502,559..689,1104..1171,
                     1232..1305,1365..1417,2039..2120,2172..2322)
                     /partial
                     /note="alternative cds; putative"
                     /product="cathepsin B-like cysteine protease"
                     /codon_start=1
                     /translation="MKYLFFALCLYLYQGISEAEVPDALEAEQIPLEAQALSGLPLVE
                     YLQKNQRLFEVTATPVPYFKQRLMDLKYIDQNNIPDEEVEDEELEENNDDIPESYDPR
                     IQWANCSSLFHIPDQANCGSCWAVSSAAAMSDRICIASKGAKQVLISAQDVVSCCTWC
                     GDGCEGGWPISAFRFHADEGVVTGGDYNTKGSCRPYEIHPCGHHGNETYYGECVGMAD
                     TPRCKRRCLLGYPKSYPSDRYY"
BASE COUNT      697 a    592 c    561 g    650 t
ORIGIN      
        1 gaattctggt gagcaaaatg ttgcgtgttg cctggaaatt atccggatca tcctcatgga
       61 aatatttgct catcatttgc ggacctaagc gtcgatgact ctccttgaga ctttccctta
      121 tcacgaagga cactgattcc tctagaaaag cggactgtag cgattataac cagatgccga
      181 cgtagccgtg aattttcgag gcgatggtga gttcccttct cgacgttctc ctaaaaaagc
      241 ctttctgata taagcaattc ttggaaatgt caaatattcc agaaatacct tttctttgca
      301 ctttgcttat acctgtatca agggataagc gaagcagaag ttcctggtta gtccccattt
      361 attcctcggt attcacttat gaagacagat attctgtcct cctgcagatg ctttggaagc
      421 tgaacaaatc ccgctggaag cgcaggcgtt gagcggtttg ccgctggttg aatatctcca
      481 aaagaaccag agactcttcg aggttgtcca tggcagactt ccatggctgt cagccgccct
      541 aaccgtgccc atttgcaggt cactgcgaca ccagtgcctt acttcaagca gaggttgatg
      601 gatctgaaat acatagacca gaataacatt cccgatgaag aggtggaaga tgaggaactg
      661 gaagaaaaca acgatgacat acctgagagg ttggttgaca gccttttgca cagcttagac
      721 actccttgaa acctataaag tataaaccta gcataaactc taccgtactc aatgacataa
      781 acattaaacg tgaacgacaa tgatcagagc tctgaaatcc catgctcccg aacttccttc
      841 gaactgtaga ggggcctttt tcaatgcatg actagaaatt gatgtggtat tggatcaata
      901 tcaaggccca cccactgagt taccaaagcc gattccctct tgaattcaga gggtcccaac
      961 cgcagcactt tggtggtaca attcagaagg ggaggggaga tcgaactcag gtgtgtggct
     1021 caactcatct tagtaatggg gaaataacgc cttgaccctc aactcatctt agtaatgggg
     1081 aaataacgcc ttgacctttt tagctacgat cctcgaattc aatgggccaa ctgctcttcg
     1141 ctctttcaca tccccgacca agcgaactgc ggtaaaacct ttcaacagtc ccggcctaca
     1201 acatcctgaa atctaggtta gcttcgtcta ggctcatgtt gggcagtctc gtcggcagct
     1261 gcgatgtctg acaggatttg catcgctagc aagggtgcca aacaggtaca gaacaatcta
     1321 cacaatagaa ttctaaattc acaacctcag ggagcacaat tcaggtgctg atctcagctc
     1381 aagacgttgt gtcgtgctgc acatggtgtg gtgatgggta cgctttttct tgactatttg
     1441 catttctgaa acggttgcgg atgatcattc tcgcactggg cactgaggtc accttccttt
     1501 ggaccttgaa gaactggtgt atgtaccagg cctattttta agctttgacc taagcctaag
     1561 tctaagcctg aactgagcct aagcccaagc ttcggcatga tcatgaccta atgtgtaagc
     1621 gagtctaaga cccaacctaa tcgcccaagt ctagacataa gcctgaactt agggtctaag
     1681 cttgagccta agcccgagac tgagcctcat agactcaacc ctaatgtgta agcataagcc
     1741 tgctcaagca caagccgacg cccaagctca attttagcct tcgatgatgc gcagctccgg
     1801 taacctaaga agcaaactga gctcctatgt ttcgttcatg tgtgtaacaa ctcgcattgg
     1861 tccgcttaac cgaaaccatc gaaacgatta cccgacaagt tttctaccca agccggatga
     1921 ttttagtgag atggaatcta aattttggta agcggcgaat tcttgtctac caaaaaaaac
     1981 atccactaaa aagatcgaca aatcgtgttt cataaaatgg cagaactttc tgattcagat
     2041 gtgaaggtgg ctggccgatc agtgctttcc gatttcacgc agatgaaggc gttgtgactg
     2101 gtggagacta caacacaaag gtgagatcac aacgagctcc ttctcgtgat ccacgtaggg
     2161 taagcattta gggtagctgc cgtccttacg agattcatcc atgtggacat catggaaatg
     2221 agacatatta tggagaatgc gttggcatgg cggatactcc acggtgcaaa aggagatgcc
     2281 tgcttggcta cccgaaatcc tatccatcgg ataggtatta cggtacgtct aaattcgaac
     2341 ttaaaggcaa tttagccctg aaactgatcg tgcgaaagat agccataacg ctagatatca
     2401 atgtgaacgt tcataagttc tcgtgcacat aataaaccga aactcagtca agagggttgt
     2461 atagcaccac agggctcagt gcgctttgtt cttgtccctt
//