From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: D.pseudoobscura Adh gene for alcohol dehydrogenase
Date: 28 Dec 92 21:27:28 GMT
Distribution: bionet
Checksum: 42182 8

LOCUS       DROADHGN52   3499 bp ds-DNA             INV       01-DEC-1992
DEFINITION  D.pseudoobscura Adh gene for alcohol dehydrogenase
ACCESSION   X62232
KEYWORDS    ADH gene; alcohol dehydrogenase.
SOURCE      Drosophila pseudoobscura DNA.
  ORGANISM  Drosophila pseudoobscura
            Eukaryota; Animalia; Metazoa; Arthropoda; Uniramia; Insecta;
            Pterygota; Neoptera; Holometabola; Diptera; Brachycera;
            Cyclorrhapha; Schizophora; Drosophiloidea; Drosophilidae.
REFERENCE   1  (bases 1 to 3499)
  AUTHORS   Schaeffer,S.W. and Miller,E.L.
  TITLE     Estimates of gene flow in Drosophila pseudoobscura determined from
            nucleotide sequence analysis of the alcohol dehydrogenase region
  JOURNAL   Genetics 132, 471-480 (1992)
  STANDARD  full automatic
COMMENT     *source: strain=PS108; *source: isolate=Stemwinder Provincial Park,
            British Columbia, *source: Canada; *source: chromosome=IV section
            88;
               From EMBL    entry DPADHGN52;  dated 16-OCT-1992.
FEATURES             Location/Qualifiers
     mRNA            join(40..119,915..1020,1095..1499,1560..1975)
                     /note="adult mRNA (Adh)"
     mRNA            join(882..1020,1095..1499,1560..1975)
                     /note="larval mRNA (Adh)"
     promoter        8..14
                     /note="adult promoter (Adh)"
     prim_transcript 40..1975
                     /note="adult primary transcript (Adh)"
     exon            40..119
                     /note="adult exon 1a"
     intron          120..914
                     /note="adult intron"
     promoter        846..853
                     /note="larval promoter (Adh)"
     prim_transcript 882..1975
                     /note="larval primary transcript (Adh)"
     exon            882..1020
                     /note="larval exon 1"
     exon            915..1020
                     /note="adult exon 1b"
     intron          1021..1094
                     /number=1
     exon            1095..1499
                     /number=2
     intron          1500..1559
                     /number=2
     exon            1560..1975
                     /number=3
     polyA_signal    1952..1957
     polyA_site      1975
     CDS             join(928..1020,1095..1499,1560..1826)
                     /EC_number="1.1.1.1"
                     /gene="Adh"
                     /product="alcoholdehydrogenase"
                     /codon_start=1
                     /translation="MSLTNKNVVFVAGLGGIGLDTSRELVKRNLKNLVILDRIDNPAA
                     IAELKAINPKVTITFYPYDVTVPVAETTKLLKTIFAQVKTIDVLINGAGILDDHQIER
                     TIAVNYTGLVNTTTAILDFWDKRKGGPGGIICNIGSVTGFNAIYQVPVYSGSKAAVVN
                     FTSSLAKLAPITGVTAYTVNPGITKTTLVHKFNSWLDVEPRVAEKLLEHPTQTSQQCA
                     ENFVKAIELNKNGAIWKLDLGTLEPITWTQHWDSGI"
BASE COUNT      983 a    799 c    798 g    919 t
ORIGIN      
        1 cgcagtgtat ttaaggcact gcacatgtcg aggaccacga ttattgtcta cagtgcagtc
       61 agtagttgtc aggtgcagca ggcggaccag catttcttta aatctactca atcgatcaag
      121 taagtaactg taatccaagt acagtcggcc catcccactc ggttctgccg taactcaaga
      181 aatttcttga tagcgcagtt ccagttgtag aacgtcgttt aaaaaaacat ctcgttggag
      241 cttgggttga aaaacgttcg agttgggcgt agaaaagttc aaatataaaa tatcaaaaaa
      301 gataaaatat cttcccaaag cagctgtcca ttttttgtta aagtgataga tcaaaaattc
      361 gtatgacgta cattcctagt gcacttcata caaacaattt cctcaattct aattctacaa
      421 gaagcacttt tggctcttgt ccagaaaaaa caatttgttt acacttttcg gatgataaca
      481 gaggcggacc gacggctaag taacataaca gtcgcctgtt aagtaatgcc ctcactctct
      541 gttagatggc cgcgcgtgtc gactccgata gaccagcaga cacgcagtag ttttcctcaa
      601 gaacctgaac tctaaacata gacataattt acgatacctt gagactcaaa taaaaataca
      661 agtatgagtg gtattctatt cctgattaga ctcgcacaca cacacgtacg agtattaaca
      721 taaatttgcc ggcaaataac caaaacaaaa cgaacgcaaa cgatccgagc acgtaatgcg
      781 agagataaga aacagaaaag ctcgacgtga aagaagcttc tggcggaagc tcttgccgaa
      841 ctccctataa atacgagaca gaaaaccaga ataaatataa taacaaactg ttgaattgtc
      901 ccatcgattc ccagatcaaa agacagaatg tctctcacca acaagaacgt cgttttcgtg
      961 gccggtctgg gcggcattgg cctggacacc agccgggagt tggtcaagcg taatctgaag
     1021 gtaagagaga gaaagagcaa tcccttttcc aaggaatcta tggaatccta aatttaaaaa
     1081 tttatatttt ttagaacctg gtcatcctgg atcgcattga caatccggct gccattgccg
     1141 agctgaaggc aatcaatccc aaggtgacca tcaccttcta tccctacgat gtgactgtgc
     1201 ccgtcgctga gaccaccaag ctcctgaaga ccatctttgc ccaggtgaag acaatcgatg
     1261 tcctgatcaa cggtgctggc atcctggacg atcatcagat cgagcgcacc attgccgtta
     1321 actacacggg cctggtcaac accaccacag ccattctgga cttctgggac aagcgcaagg
     1381 gcggcccagg cggcatcatt tgcaacattg gctccgtcac cggtttcaat gccatctacc
     1441 aggtgcccgt ttactccggc tccaaggcgg cggtggttaa ctttacctcc tccctggcgg
     1501 taagaaatcc tacaactatc ctactcccca aaactaaata tttctttgaa attccataga
     1561 aactggctcc cattactggt gtcactgctt acactgtcaa tcctggcatc actaagacca
     1621 ctctggtcca caaattcaac tcgtggctgg atgtggagcc ccgtgtggca gagaagctgc
     1681 tcgagcatcc cacccagacc tctcagcagt gcgccgagaa ctttgtgaag gccattgagc
     1741 tgaacaagaa cggtgccatc tggaagttgg atttgggcac cttggagccc atcacatgga
     1801 cccagcactg ggactcgggc atctaaacca tcccagagac tctatgggac atggcgtttt
     1861 agcttttagt ttcttttttt ccactcaatt gttacgtata tacatacaca tatggcaata
     1921 aggctgattt gaaccccgtt ttgaatatga taatacaaaa ttatatttga gaaatttcaa
     1981 caaaatcgat aagagctata gctctctttt aattaaaaaa aactagataa aaagagcaat
     2041 ggtcagtggt ggtcagtggt cgttgtggcc tctatcgatt tccacacaaa aactttactg
     2101 tttagtaata gaaaagaact tgagaaaggc agccaaaaga atgtacgatc tgacgggtaa
     2161 gcatgtctgc tatgtggctg actgcggtgg cattgcactg gagaccagca aggttctcat
     2221 gaccaagaat atagcggtga gtggtagaga ggtggtgtag agagtggaga gtgttccgaa
     2281 gtggttctgg ttcttaaaca agtctctagt ctctagtctc tagtctctag tctcttggct
     2341 gctgctagta gctgtcgaaa ctaattaagt taatgacttt ttcatgtttt gtttttgtcc
     2401 gctgactttt gctgttcaaa gctacaatta agtttatgtt ttgagtttga tctacttttg
     2461 cttaagggaa gtactttcga ttacggattg ctggcatata aaaatagata ccaaggaatg
     2521 tgcatcatat tgattctatc ttgattcttt gcctagaaac tggccattct tcagagcgtg
     2581 gaaaaccccc cggccattgc tcagttacaa tcgattaagc acagcacaca gatcttcttc
     2641 tggaccttcg atgtgaccat ggcccgcgag gagatgaaga agtacttcga tgaggtcatg
     2701 gtccagatgg actacatcga tgtactgatc aatggggcca ccctgtgcga tgagcggaac
     2761 attgatgcca ccatcaatac gaatctgacc ggcatgatga acaccgtggc cactgtgctg
     2821 ccctatatgg accgaaagat gggcggttcg ggtggactga ttgtgaatgt cacctccgtc
     2881 ataggactgg atccatcgcc ggtcttttgt gcgtacagtg cctcaaagtt tggtgtgatt
     2941 gggttcaccc gaagtctagc ggtgagtggg cgtgatctta acttgaaaat gctctctaat
     3001 atcttccatt cttccgcagg atcccctgta ttacacccaa aatggtgtgg ctgtcatggc
     3061 cgtctgctgt ggtcccacta aagtgtttgt cgatcgcgaa ctgactgcct tcctggaata
     3121 cggccaaacc tttgccgatc gcctgcgccg tgccccctgc caatcgacgg ccgtctgcgg
     3181 ccagaatata gtaaatgcca tcgagagatc ggagaacgga cagatctgga ttgccgacaa
     3241 gggcggcctg gagtcggtgg ccctccactg gtattggcat atggccgatc agtttgtgaa
     3301 ctatatgcag agcactgacg atgaggatca ggagtttttt ttgggccagc gatgaggcat
     3361 gccttgatcc acggaataaa acatcaaatg tgtcacaaaa agtaggcaac ttgtttgtgg
     3421 gtctggaaat agatttgaaa tattttgagg tcatatgaat aatgacataa atgagggtgc
     3481 ctgaaagtat gccaagttt
//