HDTenEightyP/NetNews huggingface dataset:
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human immunodeficiency virus type 2 (FOENVA11) envelope protein
Date: 18 Aug 92 10:01:27 GMT
Distribution: bionet
Checksum: 00129     2

LOCUS       HIVENVA11     453 bp ss-RNA             VRL       18-AUG-1992
DEFINITION  Human immunodeficiency virus type 2 (FOENVA11) envelope protein
            fragment.
ACCESSION   M87089
KEYWORDS    envelope protein.
SOURCE      Human immunodeficiency virus type 2 uncultured blood mononuclear
            cells (11/89 sample) RNA.
  ORGANISM  Human immunodeficiency virus type 2
            Viridae; ss-RNA enveloped viruses; Positive strand RNA virus;
            Retroviridae; Lentivirinae.
REFERENCE   1  (bases 1 to 453)
  AUTHORS   Gao,F., Yue,L., White,A.T., Pappas,P.G., Barchue,J., Hanson,A.P.,
            Greene,B.M., Sharp,P.M., Shaw,G.M. and Hahn,B.H.
  TITLE     Human infection by genetically-diverse SIVsm-related HIV-2 in West
            Africa
  JOURNAL   Nature (1992) In press
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     misc_feature    1..453
                     /note="PCR derived HIV-2 envelope fragment"
                     /gene="env"
                     /note="putative"
                     /citation=[1]
BASE COUNT      159 a     78 c    106 g    110 t
ORIGIN
        1 gacgtggtca agagacaaca agaattgttg cgactgaccg tctgggggac taaaaacctc
       61 cagactagag tcactgctat cgagaagtac ctaaaggacc aggcgcagct aaattcatgg
      121 ggatgcgcgt tcagacaggt ctgccacacc actgtagaat ggccaaatag tactctcaca
      181 cctgactgga acaatatgac ttggcaggag tgggaaagac aggttgattt cctagaggca
      241 aatataacac aattattaga ggaagcacaa attcagcagg agaaaaacat gtatgagtta
      301 caaaaactaa ataactggga tatatttggc aactggtctg accttacttc ttggattaag
      361 tatatacagt atggagtctt catagtagta ggtataatac tgttaagaat aataatctat
      421 gtagtacaaa tgttagctaa gttaagtaag gga
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Brassica napus (BAG1) mRNA, complete cds.
Date: 20 Aug 92 10:00:56 GMT
Distribution: bionet
Checksum: 27073     3

LOCUS       BNABAG1X     1098 bp ss-mRNA            PLN       20-AUG-1992
DEFINITION  Brassica napus (BAG1) mRNA, complete cds.
ACCESSION   M99415
KEYWORDS    .
SOURCE      Brassica napus (cultivar Westar) inflorescence cDNA to mRNA.
  ORGANISM  Brassica napus
            Eukaryota; Plantae; Embryobionta; Magnoliophyta; Magnoliopsida;
            Dilleniidae; Capparales; Brassicaceae.
REFERENCE   1  (bases 1 to 1098)
  AUTHORS   Mandel,M.A., Bowman,J.L., Kempin,S.A., Ma,H., Meyerowitz,E.M. and
            Yanofsky,M.F.
  TITLE     Manipulation of Flower Structure in Transpenic Tobacco
  JOURNAL   Cell (1992) In press
  STANDARD  simple staff_entry
FEATURES             Location/Qualifiers
     mRNA            1..1098
                     /gene="BAG1"
     CDS             126..884
                     /gene="BAG1"
                     /codon_start=1
                     /translation="MAYQMELGGESSPQRKAGRGKIEIKRIENTTNRQVTFCKRRNGL
                     LKKAYELSVLCDAEVALIVFSSRGRLYEYSNNSVKGTIERYKKAISDNSNTGSVAEIN
                     AQYYQQESAKLRQQIISIQNSNRQLMGETIGSMSPKELRNLEGRLDRSVNRIRSKKNE
                     LLFAEIDYMQKREVDLHNDNQLLRAKIAENERNNPSMSLMPGGSNYEQIMPPPQTQPQ
                     PFDSRNYFQVAALQPNNHHYSSAGREDQTALQLV"
     misc_signal     176..345
                     /standard_name="MADS-box"
                     /gene="BAG1"
BASE COUNT      367 a    234 c    214 g    283 t
ORIGIN
        1 caatcaacaa cttcaccctt ccattttctg caacttctcc aaatctcata ctttccagaa
       61 aatcattttc ccaagagaaa taaaactttc ctctttgttc atctctcttc cccccaacag
      121 caaacatggc ttaccaaatg gagctaggag gagaatcctc tccacaaagg aaagctggga
      181 gaggaaagat cgaaataaaa cggatcgaga acacaacgaa ccgtcaagtt actttctgca
      241 aacgcagaaa tggtttgctc aagaaagctt acgaactctc tgttctttgt gatgctgaag
      301 tcgcactcat tgtcttctct agccgtggcc gtctctatga gtactcaaac aacagtgtaa
      361 aagggacaat tgagaggtac aagaaagcaa tatcggataa ttctaacacc ggatccgtgg
      421 cagaaattaa tgcacagtat tatcaacaag aatctgccaa attgcgtcaa caaattatca
      481 gcatacagaa ctcgaacagg caattgatgg gtgagacgat tgggtcaatg tctcccaaag
      541 agctcaggaa cttggaaggc agattagaca gaagtgttaa tcgaatccga tccaagaaga
      601 acgaactctt attcgccgaa attgactaca tgcagaagag agaagttgat ttgcataacg
      661 ataaccagct tcttcgtgct aagatagctg aaaatgagag gaacaatcca agtatgagtc
      721 tgatgccagg aggatctaac tacgagcaga tcatgccacc gcctcaaacg caacctcaac
      781 cgtttgactc acggaactat ttccaagtcg cggcattgca acctaacaat caccattact
      841 catccgcagg tcgcgaagac caaaccgctc ttcagttagt gtaatattgg ctgaagcatg
      901 aaggagcaag gactgaataa aaaccagaac tgggttaagg aacgagcgat ataaagctga
      961 tgcactgtta taaaaatatt tatatattta tttcacgaat gttgtgtcca tgctttctac
     1021 attttattta aattgcttat gttgatgtga aattaatatc ttaaaagaca tgtgattaat
     1081 gtgctttaat ttgtttcg
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Rhesus macaque polyoma virus large T antigen gene, 3' end.
Date: 22 Aug 92 10:01:36 GMT
Distribution: bionet
Checksum: 38493     3

LOCUS       SV4LTA2       337 bp ds-DNA             VRL       22-AUG-1992
DEFINITION  Rhesus macaque polyoma virus large T antigen gene, 3' end.
ACCESSION   M99351
KEYWORDS    large T antigen.
SOURCE      Rhesus macaque polyomavirus (strain Br54) DNA.
  ORGANISM  Rhesus macaque polyomavirus
            Viridae; ds-DNA nonenveloped viruses; Papovaviridae;
            Polyomaviruses.
REFERENCE   1  (sites)
  AUTHORS   van Heuverswyn,H. and Fiers,W.
  TITLE     Nucleotide sequence of the Hind-C fragment of Simian Virus 40 DNA:
            comparison of the 5'-untranslated region of wild-type virus and of
            some deletion mutants
  JOURNAL   Eur. J. Biochem. 100, 51-60 (1979)
  STANDARD  full staff_review
REFERENCE   2  (bases 1 to 337)
  AUTHORS   Ilyinskii,P.O., Daniel,M.D., Horvath,C. and Desrosiers,R.C.
  TITLE     Genetic analysis of SV40 from brain and kidney of macaque monkeys
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     CDS             complement(8..337)
                     /note="partial"
                     /product="large T antigen"
                     /codon_start=1
                     /translation="LDKEFSLSVYQKVKFNVAMGIGVLDWLRNSDDDDEDSQENADKN
                     EDGGEKNMEDSGHETGIDSQSQGSFQAPQPSQSSQSVHDHNQPYHICRGFTCFKKPPT
                     PPPEPET"
                     /partial
     variation       replace(68,"a")
                     /note="nucleotide 2751 in SV40 strain 776"
                     /citation=[1]
     variation       replace(74,"g")
                     /note="nucleotide 2757 in SV40 strain 776"
                     /citation=[1]
     mutation        replace(110..120,"ct")
                     /note="absent in SV40 strain 776"
                     /citation=[1]
     variation       replace(188,"c")
                     /note="nucleotide 2862 in SV40 strain 776"
                     /citation=[1]
     variation       replace(221,"t")
                     /note="nucleotide 2895 in SV40 strain 776"
                     /citation=[1]
     variation       replace(301,"t")
                     /note="nucleotide 2975 in SV40 strain 776"
                     /citation=[1]
BASE COUNT       85 a     75 c     65 g    112 t
ORIGIN
        1 attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag caagtaaaac
       61 ctctacagat gtgatatggc tgattatgat catgaacaga ctgtgaggac tgtgagggct
      121 gaggggcctg aaatgagcct tgggactgtg aatcaatgcc tgtttcatgc cctgagtctt
      181 ccatgttttt ctccccacca tcttcatttt tatcagcatt ctcctggctg tcttcatcat
      241 catcatcact gtttcttagc caatctaaaa ctccaattcc catagccaca ttaaacttca
      301 ctttttgata cactgacaaa ctaaactctt tgtccaa
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human chromosome 4 sequence-tagged site STS4-228
Date: 21 Aug 92 10:07:55 GMT
Distribution: bionet
Checksum: 24491     3

LOCUS       HUM4STS228    621 bp ds-DNA             PRI       21-AUG-1992
DEFINITION  Human chromosome 4 sequence-tagged site STS4-228
ACCESSION   M95278
KEYWORDS    STS; human chromosome 4; sequence tagged site.
SOURCE      Homo sapiens, clone C4-84 from Los Alamos National Laboratory
            chromosome 4 cosmid library, plate 4-1-1R, pos 6C; vector SuperCos
            1 (Stratagene)
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 621)
  AUTHORS   Goold,R.D., diSibio,G., Dugaiczyk,A., Smith,K.A., Xu,H.,
            Magrane,G., Lang,D.B., Cox,D.R., Masters,S.B. and Myers,R.M.
  TITLE     Sequence-Tagged Sites for Human Chromosome 4
  JOURNAL   Unpublished (1992) see COMMENT for author address
  STANDARD  full automatic
COMMENT     PCR components: 25 ng of human genomic DNA, 10 pmol of each
            oligonucleotide, 200 micro-M dNTPs, 0.25 U Taq  polymerase (Cetus)
            in 10 micro-l of 50 mM KCl-20 mM  Tris-HCl, pH 8.3 (at room temp),
            2.5 mM MgCl-2.  Initial denaturation at 94degC for 1.5 min, then 30
            cycles of  94degC for 15 sec, 62degC for 23 sec, and 72degC for 30
            sec, followed by a final extension at 72degC for 3.5 min, using a
            Perkin-Elmer 9600 thermocycler. PCR-amplified product size 244 bp.
            Sequence submitted by:
            Human Genome Mapping Center
            Box 0925
            University of California San Francisco
            San Francisco, CA 94143-0925  USA
            Phone: (415) 502-1612   Fax: (415) 476-8391
            e-mail: hgmcprobes@cgl.ucsf.edu
FEATURES             Location/Qualifiers
     misc_feature    61..86
                     /label=Primer1
     misc_feature    complement(280..304)
                     /label=Primer2
BASE COUNT      167 a    128 c    124 g    162 t     40 others
ORIGIN
        1 ctcacactct tgtgagcaaa aaacataatt tattanttta attngagtac aatgagtgag
       61 gatagataat atntggagca ggtttctncc tgaattaatg agggcactga aatctgaatg
      121 tnttgtccta tagaaataaa aagttgacag gtaggnttga cctcagacaa acagctcaaa
      181 caaagctggt gagnttctcc atcatgtgcc agttcaatca gtggacttta taacatgaaa
      241 ctncaacctc ccaaattcag gtgccttaaa caatggcata gcacttacaa atctcacatt
      301 atggaaangg gagagattct tttcctnata ttcattcaca agaaaagaag agtccctcca
      361 cctcggnagc tttancagtt ctgacctngt ntcatattgg tttccacctg gttacatgtc
      421 caacccctan nnttntccct ggtggcaaag ttgatgagng gtgggcagtn anaccccnac
      481 ancaagcagn gntngggaaa caggctctcc caaatctttg gtttggtcng tttgagnggg
      541 attngttcct ccacgaacag gnggtttngg gncttttncc ccnatnggag gtcaacggng
      601 gnaacnttcc ccccttcctn n
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human immunoglobulin heavy chain variable region, diversity region,
Date: 20 Aug 92 10:03:40 GMT
Distribution: bionet
Checksum: 64060     2

LOCUS       HUMIGVDJX     309 bp ds-DNA             PRI       20-AUG-1992
DEFINITION  Human immunoglobulin heavy chain variable region, diversity region,
            joining region (IGH@) gene.
ACCESSION   M99372
KEYWORDS    diversity region; heavy chain; immunoglobulin; joining region;
            variable region.
SOURCE      Homo sapiens blood DNA.
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (sites)
  AUTHORS   Schroeder,H.W.Jr., Hillson,J.L. and Perlmutter,R.M.
  TITLE     Early restriction of the human antibody repertoire
  JOURNAL   Science 238, 791-793 (1987)
  STANDARD  full staff_review
REFERENCE   2  (sites)
  AUTHORS   Deane,M. and Norton,J.D.
  TITLE     Immunoglobulin heavy chain variable region family usage is
            independent of tumor cell phenotype in human B lineage leukemias.
  JOURNAL   Eur. J. Immunol. 20, 2209-2217 (1990)
  STANDARD  full staff_review
REFERENCE   3  (bases 1 to 309)
  AUTHORS   Singal,D.P.
  JOURNAL   Unpublished (1992)
  STANDARD  full staff_entry
BASE COUNT       73 a     88 c     78 g     70 t
ORIGIN
        1 acctgtgcca tctccgggga cagtgtctct agcaacagtg ctgcttggaa ctgcatcagg
       61 cagtccccat cgagaggcct tgagtggctg ggaaggacat actacaggtc catctggtat
      121 cgtgattatg cagtatctgt gaaaagtcga ataaccatca acccagacac atccaagaac
      181 cagttctccc tgcagctgaa ctctgtgact cccgaggaca cggctatata tttctgtgca
      241 agagaccatg gtgggacctt ctacttcgac ccctggggcc agggaaccct ggtcaccgtc
      301 tcctcaggt
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human Ig germline H-chain gene V region, partial cds.
Date: 22 Aug 92 10:00:35 GMT
Distribution: bionet
Checksum: 44401     2

LOCUS       HUM15IGVH     434 bp ds-DNA             PRI       22-AUG-1992
DEFINITION  Human Ig germline H-chain gene V region, partial cds.
ACCESSION   M99411
KEYWORDS    immunoglobulin heavy chain subgroup VH-III;
            immunoglobulin heavy chain variable region.
SOURCE      Homo sapiens (individual_isolate LS) blood DNA.
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 434)
  AUTHORS   Adderson,E.E., Carroll,W.L. and Shackelford,P.G.
  TITLE     The human VH3b gene subfamily is highly polymorphic
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     CDS             join(1..13,117..434)
                     /product="immunoglobulin heavy chain variable region"
                     /gene="IGHV@"
                     /codon_start=1
                     /translation="AILKGVQCEVQLVESGGGLVKPGGSLRLSCAASGFTFSNAWMSW
                     VRQAPGKGLEWVGRIKSKTDGGTTDYAAPVKGRFTISRDDSKNTLYLQMNSLKTEDTA
                     VYYCTTDT"
                     /partial
     sig_peptide     1..13
                     /gene="IGHV@"
                     /codon_start=1
                     /partial
     intron          14..116
                     /gene="IGHV@"
BASE COUNT      115 a     83 c    133 g    103 t
ORIGIN
        1 gctattttaa aaggtgattt atggagaact agagagatta agagtgagtg gatatgagtg
       61 agagaaacag tggatatgtg tggcagtttc tgatcttagt gtctctgtgt ttgcaggtgt
      121 ccagtgtgag gtgcagctgg tggagtctgg gggaggcttg gtaaagcctg gggggtccct
      181 tagactctcc tgtgcagcct ctggattcac tttcagtaac gcctggatga gctgggtccg
      241 ccaggctcca gggaaggggc tggagtgggt tggccgtatt aaaagcaaaa ctgatggtgg
      301 gacaacagac tacgctgcac ccgtgaaagg cagattcacc atctcaagag atgattcaaa
      361 aaacacgctg tatctgcaaa tgaacagcct gaaaaccgag gacacagccg tgtattactg
      421 taccacagac acag
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human immunodeficiency virus type 2 (FOENVC11) envelope protein
Date: 18 Aug 92 10:01:51 GMT
Distribution: bionet
Checksum: 15399     2

LOCUS       HIVENVC11     453 bp ss-RNA             VRL       18-AUG-1992
DEFINITION  Human immunodeficiency virus type 2 (FOENVC11) envelope protein
            fragment.
ACCESSION   M87105
KEYWORDS    envelope protein.
SOURCE      Human immunodeficiency virus type 2 uncultured blood mononuclear
            cells (3/90 sample) RNA.
  ORGANISM  Human immunodeficiency virus type 2
            Viridae; ss-RNA enveloped viruses; Positive strand RNA virus;
            Retroviridae; Lentivirinae.
REFERENCE   1  (bases 1 to 453)
  AUTHORS   Gao,F., Yue,L., White,A.T., Pappas,P.G., Barchue,J., Hanson,A.P.,
            Greene,B.M., Sharp,P.M., Shaw,G.M. and Hahn,B.H.
  TITLE     Human infection by genetically-diverse SIVsm-related HIV-2 in West
            Africa
  JOURNAL   Nature (1992) In press
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     misc_feature    1..453
                     /note="PCR derived HIV-2 envelope fragment"
                     /gene="env"
                     /note="putative"
                     /citation=[1]
BASE COUNT      180 a     76 c     86 g    111 t
ORIGIN
        1 gacgtggtca agagacaaca agaattgttg caactaaccg tctaggaaac taaaaacctc
       61 cagactaaag tcactgctat cgaaaagtac ctaaaggacc aggcacagct aaattcatgg
      121 ggatgcgcgt tcaggcaggt ctgccacacc actgtaaaat ggccaaatag tactctcaca
      181 cctgactgaa acaatatgac ttggcagaag tgagaaagaa aggttaattt cctaaaggca
      241 aatataacac agttgttaga ggaagcacaa attcaacagg agaaaaacat gtataagtta
      301 caaaaactaa ataactgaaa tgtatttagc aactggtttg accttacttc ttaaatcaag
      361 tatatacagt ataaagtctt catagtaata ggtataatat tgttaaaaat agtaatctat
      421 gtagtacaaa tgttagctaa gttaagtaag gga
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human immunodeficiency virus type 2 (22ENVBB5) envelope (gp 41)
Date: 18 Aug 92 10:01:15 GMT
Distribution: bionet
Checksum: 33864     2

LOCUS       HIV2ENVBB5    453 bp ss-RNA             VRL       18-AUG-1992
DEFINITION  Human immunodeficiency virus type 2 (22ENVBB5) envelope (gp 41)
            fragment.
ACCESSION   M87128
KEYWORDS    envelope protein; glycoprotein.
SOURCE      Human immunodeficiency virus type 2 uncultured blood mononuclear
            cells (5/89 sample) RNA.
  ORGANISM  Human immunodeficiency virus type 2
            Viridae; ss-RNA enveloped viruses; Positive strand RNA virus;
            Retroviridae; Lentivirinae.
REFERENCE   1  (bases 1 to 453)
  AUTHORS   Gao,F., Yue,L., White,A.T., Pappas,P.G., Barchue,J., Hanson,A.P.,
            Greene,B.M., Sharp,P.M., Shaw,G.M. and Hahn,B.H.
  TITLE     Human infection by genetically-diverse SIVsm-related HIV-2 in West
            Africa
  JOURNAL   Nature (1992) In press
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     misc_feature    1..453
                     /note="PCR derived HIV-2 envelope (gp 41) fragment"
                     /note="putative"
                     /citation=[1]
BASE COUNT      154 a     75 c    112 g    112 t
ORIGIN
        1 gacgtggtca aaagacaaca ggaaatgttg cggctgaccg tctggggaac taaaaacctc
       61 cagactagag tcactgccat cgagaagtac ctaaaggacc aagcaaaact aaattcatgg
      121 ggatgtgcct ttagacaggt ttgccacact actgtgccat gggtgaacga cagtatgcag
      181 ccagagtggc aaaacatgac ttggcaacaa tgggaaaaac agattgcctt cttggaggat
      241 aatatcacag agctgttaga gcaagctcaa atacagcagg agaaaaatat gtatgagttg
      301 cagaaattaa atagctggga tgtttttggc aattggcttg atctcacctc ttgggtaaaa
      361 tatatctatt taggatttta tatagtagca ggagtaatag tgttaagaat agctatctat
      421 gtagtacaaa tgttaatgag acttaggaag ggc
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: 57-VH
Date: 13 Aug 92 10:00:53 GMT
Distribution: bionet
Checksum: 03819     3

LOCUS       MUSD12732     358 bp ss-mRNA            ROD       13-AUG-1992
DEFINITION  57-VH
ACCESSION   D12732
KEYWORDS    .
SOURCE      Mus musculus (strain BALB/c) mRNA.
  ORGANISM  Mus musculus
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Rodentia; Myomorpha; Muridae; Murinae.
REFERENCE   1  (bases 1 to 358)
  AUTHORS   Sawada,J., Mizusawa,S., Terao,T., Naito,M. and Kurosawa,Y.
  TITLE     Molecular characterization of monoclonal anti-steroid antibodies:
            primary structures of the variable regions of seven antibodies
            specific for 17alpha-hydroxyprogesterone or 11-deoxycortisol and
            their pH-reactivity profiles
  JOURNAL   Mol. Immunol. 28, 1063-1072 (1991)
  STANDARD  full automatic
COMMENT     Submitted (27-JUL-1992) to DDBJ by:
            Jun-ichi Sawada
            Dept. of Biochem. and Immunochem.
            National Institute of Hygienic Sciences
            1-18-1 Kamiyoga
            Setagaya, Tokyo 158
            Japan
            Phone:  03-3700-1141 x240
            Fax:    03-3707-6950
FEATURES             Location/Qualifiers
     CDS             <1..>358
                     /product="'Ig gamma chain precursor'"
                     /codon_start=1
                     /translation="DVQFQESGPGLVKPSQSLSLTCTVTGHSITSDHVWNWIRQFPGN
                     KLEWMAYITYSGSIGYNPSLTSRISVTRDTSKNQFFLQLNSVTTEDTATYYCTRGNSL
                     YYAMDYWGRGISVTVSS"
     mat_peptide     1..>358
                     /note="'VDJ region (1-358)'"
                     /product="'Ig gamma chain'"
                     /codon_start=1
BASE COUNT       88 a     95 c     80 g     95 t
ORIGIN
        1 gatgtgcagt ttcaggagtc gggacctggc ctggtgaaac cttctcagtc tctgtccctc
       61 acctgcactg tcactggtca ctcaatcacc agtgatcatg tctggaactg gatccggcag
      121 tttccaggaa acaagctgga gtggatggcc tatataacct acagtggtag tattggctac
      181 aacccatctc tcacaagtcg aatctctgtc actcgagaca catccaagaa tcagttcttc
      241 ttgcagttga attctgtgac tactgaggac acagccacat attactgtac aagaggtaac
      301 tctctctact atgctatgga ctactggggt cgaggaatct cagtcaccgt ctcctcag
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human chromosome 4 sequence-tagged site STS4-181
Date: 21 Aug 92 10:07:26 GMT
Distribution: bionet
Checksum: 47013     3

LOCUS       HUM4STS181    426 bp ds-DNA             PRI       21-AUG-1992
DEFINITION  Human chromosome 4 sequence-tagged site STS4-181
ACCESSION   L00740
KEYWORDS    STS; human chromosome 4; sequence tagged site.
SOURCE      Homo sapiens, clone p4-111 from a chromosome 4 plasmid library;
            vector pBluescript II KS + (Stratagene)
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 426)
  AUTHORS   Goold,R.D., diSibio,G., Dugaiczyk,A., Smith,K.A., Xu,H.,
            Magrane,G., Lang,D.B., Cox,D.R., Masters,S.B. and Myers,R.M.
  TITLE     Sequence-Tagged Sites for Human Chromosome 4
  JOURNAL   Unpublished (1992) see COMMENT for author address
  STANDARD  full automatic
COMMENT     PCR components: 25 ng of human genomic DNA, 10 pmol of each
            oligonucleotide, 200 micro-M dNTPs, 0.25 U Taq  polymerase (Cetus)
            in 10 micro-l of 50 mM KCl-20 mM  Tris-HCl, pH 8.3 (at room temp),
            2.5 mM MgCl-2.  Initial denaturation at 94degC for 1.5 min, then 30
            cycles of  94degC for 15 sec, 62degC for 23 sec, and 72degC for 30
            sec, followed by a final extension at 72degC for 3.5 min, using a
            Perkin-Elmer 9600 thermocycler. PCR-amplified product size 123 bp.
            Sequence submitted by:
            Human Genome Mapping Center
            Box 0925
            University of California San Francisco
            San Francisco, CA 94143-0925  USA
            Phone: (415) 502-1612   Fax: (415) 476-8391
            e-mail: hgmcprobes@cgl.ucsf.edu
FEATURES             Location/Qualifiers
     misc_feature    75..97
                     /label=Primer1
     misc_feature    complement(174..197)
                     /label=Primer2
BASE COUNT       96 a    111 c    112 g     97 t     10 others
ORIGIN
        1 gccaaaagtn ggtaccgncc cccccctcga ggtcgacggt atcgataagc ttgatatcga
       61 attcctgcag cccccacaag ttcaaccagg tcctactggg ctccagtcaa gatgtggacc
      121 gggtcgtttc tctcccaggg ntctaggaaa gaatccattc tcttgccttc tccagcttct
      181 agagttggaa tgcagaggct gcctgccatc cgtggatcag gccagttttc caacaccatt
      241 tataaaataa ggaatcctnc ccccattggt tgttnttgnc aggtttgtca aagatcccgg
      301 tactggngga gcaaatgggc tacctgccgg ggatgatccc tgtggttcac tgttgactaa
      361 gaacaaggna agctaggagc agnagagtga ngcccagggg gggatccact agttctagag
      421 cggccg
//