From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Xenopus laevis DNA topoisomerase I (TOP1) mRNA, complete cds.
Date: 29 Dec 92 20:59:16 GMT
Distribution: bionet
Checksum: 23022 8

LOCUS       XELTOPOIS    4020 bp ss-mRNA            VRT       11-DEC-1992
DEFINITION  Xenopus laevis DNA topoisomerase I (TOP1) mRNA, complete cds.
ACCESSION   L07777
KEYWORDS    DNA topoisomerase I.
SOURCE      Xenopus laevis embryo to tadpole cDNA to mRNA.
  ORGANISM  Xenopus laevis
            Eukaryota; Animalia; Chorqata; Vertebrata; Amphibia; Lissamphibia;
            Anura; Archeobatrachia; Pipoidea; Pipidae; Xenopodinae.
REFERENCE   1  (bases 1 to 4020)
  AUTHORS   Pandit,S. and Sternglanz,R.
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     polyA_signal    4001..4006
                     /gene="TOP1"
     polyA_site      4020
                     /gene="TOP1"
     CDS             112..2601
                     /gene="TOP1"
                     /product="DNA topoisomerase I"
                     /codon_start=1
                     /translation="MSEDHVQNDSQIEAVFRVNDSHKHKKDKEHRHKEHKKDKDREKS
                     KHNNSEHRDPSEKKHKDKHKNNDKHREKDGEKHRERDGEKHRDKNGEKHRDGEKHKEK
                     DIEKHKEVEKHRVKDGEKHKEKDVEKHKEKDVEKHRDGEKHKHRDKDREKKKEEKMKS
                     SSGGVKVKKENGFSSPVRVKDEPEDQGFYVSPKENKAMKRPREDDEDYKPKKIKSEDD
                     KKGKKRKQEEEDIKPKKKSKAKGNEEGVKKKKVKKEEEEKWKWWEEERHRDGIKWKFL
                     EHKGPVFAPPYEPVPDNVKFYYDGNLVKLSPKAEEVATFFAKMLDHEYTTKDIFRKNF
                     FKDWKKEMTTDERNLITNLSKCDFNAMSLYFKEQSEARKNMTKEEKLKIKAENERLLQ
                     EYGYCIMDNHKERIANFRIEPPGLFRGRGDHPKMGKLKKRIMPEDIIINCSKDSKIPV
                     APAGHKWKEVRHDGKVTWLVSWTENIQGSIKYIMLNPSSRIKGEKDWQKYETARRLKM
                     CVEKIRNTYKEDWKSKEMKVRQRAVALYFIDKLALRAGNEKEEGETADTVGCCSLRVE
                     HINLFQELDGQEFVVEFDFPGKDSIRYYNKVPVEKRVFKNLQLFMENKQPDDDLFDRL
                     NTSILNKHLQDLMEGLTAKVFRTYNASITLQQQLDELTNSDDNVPAKILSYNRANRAV
                     AILCNHQRAPPKTFEKSMMNLQGKIDAKKDQLADARREFKSAKADAKVRRDEKTKKLV
                     ESKKKAVQRIEEQLMKLEVQATDREENKQIALGTSKLNYLDPRISVAWCKKYGVPIEK
                     IYNKTQRKNLLGPSIWQTTTSNFNAEQRCFS"
BASE COUNT     1365 a    757 c    907 g    991 t
ORIGIN      
        1 gtgcctccct ccaccattgt accctttgta gccgctgtcc cgctacagac ctccctccct
       61 caccgccccc ccattcattc accgggcccc ctacttgtga tccttccaag gatgagtgag
      121 gatcatgtac agaacgactc gcagatcgag gctgtttttc gggtaaatga ctctcacaaa
      181 cacaagaaag acaaagaaca tcgccacaaa gaacacaaga aagacaaaga ccgtgaaaaa
      241 tccaaacaca acaacagtga acacagggat ccttcagaaa agaagcataa agataaacac
      301 aaaaacaatg ataaacatcg ggagaaagat ggggagaaac acagagaaag agatggggag
      361 aaacataggg acaaaaatgg ggagaaacac agagatggag aaaaacacaa agagaaagac
      421 attgagaaac acaaggaagt tgagaaacat cgggtaaagg atggagagaa acacaaagag
      481 aaggatgtgg agaagcacaa agagaaggat gtggagaagc atcgagatgg agaaaagcat
      541 aaacacagag ataaagatcg ggagaagaag aaagaggaaa aaatgaaatc ttccagcggc
      601 ggtgttaaag tgaaaaaaga aaacggtttc tctagtcctg tgcgagttaa ggatgagcca
      661 gaagatcaag gcttctacgt gtcccccaag gaaaacaagg caatgaagag gccaagagag
      721 gatgatgagg attacaaacc taagaagata aagtccgaag atgataaaaa agggaaaaag
      781 cgaaaacaag aggaagagga tattaagccc aagaaaaaaa gtaaggccaa aggcaatgaa
      841 gaaggagtga aaaagaaaaa agtcaaaaag gaggaggaag agaagtggaa atggtgggag
      901 gaggagcggc accgcgatgg cattaagtgg aagttcttgg aacataaagg tcctgtgttt
      961 gctcccccct atgaacccgt cccagacaat gtgaaatttt actatgacgg taatcttgta
     1021 aagcttagcc caaaagctga agaagtggcc acgttctttg caaaaatgtt ggatcacgag
     1081 tacactacta aggacatctt tcgcaaaaac ttcttcaaag actggaagaa ggagatgacg
     1141 actgatgaaa gaaacctcat caccaacttg agcaaatgcg acttcaatgc gatgagcctg
     1201 tactttaaag agcaatccga agcaaggaag aatatgacca aggaagaaaa gctgaaaatc
     1261 aaagcagaga acgagcggtt attgcaggag tacggctact gtatcatgga caaccacaag
     1321 gaacgtattg ctaactttcg tatagaacct cctggtctgt ttaggggcag aggagatcac
     1381 cccaaaatgg gcaaacttaa gaaaaggata atgcctgaag acatcataat caactgcagc
     1441 aaagactcaa aaattcctgt tgctcctgct ggtcacaagt ggaaagaggt tcggcatgat
     1501 ggcaaggtca catggctggt atcatggact gaaaacattc agggttctat caaatacatc
     1561 atgctcaacc ctagctcccg aatcaagggt gagaaggact ggcagaaata tgagactgcc
     1621 cgaaggctca agatgtgtgt tgaaaagatt cgaaatacct acaaagaaga ctggaagtcc
     1681 aaagagatga aagttcggca gagagcagta gctctctact ttatagacaa gcttgctttg
     1741 agagctggca atgaaaagga agagggagaa accgcagata cagtaggttg ttgctccttg
     1801 cgagtagaac acatcaatct gttccaagag ttggacggcc aagagtttgt ggtggagttt
     1861 gacttcccgg gtaaagattc catccgctac tacaacaagg tgcctgtgga gaaaagggtt
     1921 ttcaaaaatc ttcagctctt catggaaaac aagcagcctg acgatgacct gtttgacaga
     1981 ctcaatacaa gcattttgaa caagcacctt caggacctga tggagggact aacagccaaa
     2041 gtattcagaa catacaacgc ctccatcacc ctgcagcaac agctagatga gcttactaac
     2101 tctgatgaca acgttcccgc caagatcttg tcctataacc gtgccaacag agcagtcgcc
     2161 atcctgtgta accatcagcg ggcccctcct aaaacatttg agaaatctat gatgaaccta
     2221 cagggcaaga tagatgcgaa gaaggatcag ttggcagatg ccaggaggga gttcaagagt
     2281 gcgaaagcag atgcgaaggt caggcgggat gaaaaaacca aaaagcttgt agaaagcaaa
     2341 aagaaagccg tgcagaggat agaagaacag ctgatgaagt tggaggtaca agcaacagac
     2401 agggaagaaa acaaacagat tgcccttgga acctccaaac tcaactacct ggatcccaga
     2461 atctctgtgg catggtgtaa aaaatatggt gttcctattg aaaaaatcta caacaaaacc
     2521 cagaggaaaa atttgcttgg gccctcgata tggcagacga cgacttcaaa ttttaatgca
     2581 gaacagcgtt gtttttctta aattttatta ttttaagcct ggatgtcgcc tccgaaaggg
     2641 gacttttagg gaactgtgag ccttactttt ttgaatttta attatgattt tttttttttt
     2701 gtgtgcgtta aagctgggaa ggggatatgt acagattaag aaatgatggc tgagaaaaat
     2761 ccctctcccc ccccccacac ccgaatgaaa attgaattgg aggtgactga acaatcttcc
     2821 ccgagctgcc ttttgtattg cccatggaca tttagtatat aatcggatca catcaaatat
     2881 ggcaacgttt aaaatcttgg gggtgggagc gagaggttta tatgtaagaa acaaaaacat
     2941 tgcagattgt gttctagagg ctgtacagca tttacaagag aaacatgcat ttcatttttt
     3001 tttctttttt aaaatagcat ctattttaat gggggggggg atctttctgc tcatatcccc
     3061 ttaccctgaa ttcagttttt tttttttctt gttctgctga tcgatgagct tgtcttgctg
     3121 gcagctgagg ggttaattta actctcttct atccaactaa cttgatgcat agattccgac
     3181 caatcataga atttctgttt tgctactttt acaatgtgac catttttaac ccctccttca
     3241 gatatccagt ggaagaaaag cagatggtat catttttttt gggttttaga aatgtgaatt
     3301 ttttttttct taattatttt aactatttaa accttttaat atgtttggta gcatcaaaaa
     3361 aaaaaacaag aaagattcca caaacattga attttatgct tctacatttt gaattccaat
     3421 atggggaaag ggggattatc cttaaagtgc aatggagacc aaaaaaaaaa ccctttcctg
     3481 tggctgaagt ttgtgtaagg gagactcctg ctgcatgctg ggtataatcc cactactgac
     3541 accggttctg ttcctgcggt ggctcacgtg gagctcaaga aactgttgag acccctgcaa
     3601 tcgctaaggg aaatttaact tgatgaataa ccccattttt aaaggtcaaa ataacgattg
     3661 gtggatttgt tttgcatata aagaacttga aatccactca gcacaggatt cgctactgta
     3721 ttcctataaa ggcactagtg taaatgcaat ggaagacctt ttttttttct ctgtaacatg
     3781 tctggaaaat gaataaggcg agggaaaaga aacttgtaaa attttaatgt gaatatatat
     3841 tttttttttt tgcctagaat cctccatttt agttttgatt cttagatcaa ttccaaagtg
     3901 ttcagagcag tcttttcttt tttttttcct tggacaattt tcccctttta tcttctattt
     3961 ttatcatgaa tttgccatta atggagtgta ggttatttaa aataaaaccc tacaacttgc
//