From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Bacteriphage US3 lytic-enzyme (lytA) gene, complete cds.
Date: 28 Aug 92 10:01:18 GMT
Distribution: bionet
Checksum: 11331     4

LOCUS       US3LYTA      1619 bp ds-DNA             PHG       28-AUG-1992
DEFINITION  Bacteriphage US3 lytic-enzyme (lytA) gene, complete cds.
ACCESSION   M90423
KEYWORDS    aminase; lytA gene; phage lysin.
SOURCE      Bacteriophage US3 DNA.
  ORGANISM  Bacteriophage US3
            Viridae; Nonclassified viruses.
REFERENCE   1  (bases 1 to 1619)
  AUTHORS   Platteeuw,C. and de Bos,W.M.
  TITLE     Location, characterization and expression of lytic enzyme-encoding
            gene, lytA, of Lactococcus lactis bacteriophage phi US3
  JOURNAL   Gene 118, 115-120 (1992)
  STANDARD  full staff_entry
FEATURES             Location/Qualifiers
     RBS             348..353
     CDS             363..563
                     /note="ORF2"
                     /product="amidase"
                     /codon_start=1
                     /translation="MVTRMILITILILAILFATWVKDREAMNPPFKRRLVIDLTVVFA
                     LWVLYAVFYFTQTPSTSDIAKQ"
     RBS             693..701
                     /gene="lytA"
     CDS             712..1488
                     /product="amidase"
                     /gene="lytA"
                     /codon_start=1
                     /translation="MNITNAGVRGYNPTGVVIHNDAGSNGAKTSFYDSWLPNHDPEEG
                     FAHVYIASDGRLQASDFSNMAWHCANSYGNANYASWEVCQSEGDLNQFLRNEQAVLDD
                     VAKYMKQWGLTPNRDTVKLHQELSSTSCPRRSVEVHGGTVESCRSYFIAELNKRLTGQ
                     TSTIVNNTQTNTELEDDDLMKFTYQVNTKDGKPAGGVFYFNGTKVIGFTNADQWTIVK
                     QIYKDTTGKDLKHYVWNDGAPWHLRFLQANNIKVEMAPNK"
     misc_feature    1494..1511
                     /note="termination loop"
                     /gene="lytA"
BASE COUNT      525 a    279 c    322 g    493 t
ORIGIN
        1 gaattcttgc ttctggtgct tcgttgttac ttgacggaga tgttgtcaat gtttctaaaa
       61 atatatcaga ctgtgctaat ggttggatta ttcacttcac agagtttaaa tcacgtatga
      121 atggaaacac aagaaactca cttaatcagt ggttctttat tcctaaagaa tcagtacagt
      181 tagctgatgt aggacattct ttcgctcttg ctaattctac tggcggtgtt gtaactaaat
      241 ttgcatattt gcaaggtaat cgaatcacag gtcatggggt aaacaataac acatcttcaa
      301 aacaattcgc actacaacac gtattggaat attgataaat ataatttaga aagcaaacaa
      361 aaatggtaac tagaatgatt ttaataacta tcttaatttt ggcgatttta ttcgctacgt
      421 gggtcaaaga tagagaagcg atgaacccac ctttcaaacg tagacttgtg attgatttga
      481 cggtagtctt cgcgctatgg gttttatatg cagtctttta cttcacacaa acaccctcaa
      541 cttctgatat cgctaaacag tgattgacgt aggcttgttg tacttcgtag gacaatttat
      601 ttacttaatc gcaaaaatta gccctatgtt tgacggtttg gttaaactta tgaaaaagaa
      661 tggtttaagt gttcctgaag cggaagaaga acaaacggag gataaaaaag aatgaatata
      721 actaacgctg gtgtacgtgg ttataatcct actggggttg taattcataa tgacgctggt
      781 tcaaacggtg ctaaaactag tttttatgat agttggttac ctaatcatga tccagaagag
      841 ggctttgctc atgtatatat tgcttctgac ggacgattgc aggcttctga cttctctaat
      901 atggcatggc attgcgctaa ctcatacggt aatgcaaatt atgccagttg ggaagtatgc
      961 caatcagaag gcgatttaaa tcagttcttg aggaatgaac aagcggtact agatgacgta
     1021 gctaagtata tgaaacaatg gggactaact cctaatcgtg atactgtgaa gctacatcag
     1081 gagttatcat ctacttcatg tcctagacgt tccgtagagg tacatggtgg cacggtggaa
     1141 agttgtcgct catactttat cgcagaatta aataagcgcc ttacagggca aactagcaca
     1201 atagtaaaca atacacaaac aaatacagaa ttagaggacg atgatttaat gaaatttaca
     1261 tatcaagtta atacaaaaga cggaaaacca gctggcggag tattctactt caatggaaca
     1321 aaagtaattg gcttcactaa tgccgaccaa tggactatcg ttaaacaaat ttataaagat
     1381 acgacaggaa aagaccttaa acattatgtt tggaatgacg gagcgccttg gcacttgcgt
     1441 ttcttacaag ctaataacat caaagttgaa atggcaccga acaaataaaa aagaccacct
     1501 taattggtgg ttttcttttg taattgaaga tatcctactt tctatttttt aatttattgt
     1561 tttaccatgt ctcccatact gtaccgcctg aaccttggta tatgcttaca ggggaattc
//