From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: C.freundii tpl gene for tyrosine phenol-lyase
Date: 4 Sep 92 10:02:43 GMT
Distribution: bionet
Checksum: 46055    14

LOCUS       CFRTPLG      2392 bp ds-DNA             BCT       04-SEP-1992
DEFINITION  C.freundii tpl gene for tyrosine phenol-lyase
ACCESSION   X66978
KEYWORDS    lyase.
SOURCE      Citrobacter freundii DNA.
  ORGANISM  Citrobacter freundii
            Prokaryotae; Gram-negative facultatively anaerobic rods;
            Enterobacteriaceae.
REFERENCE   1  (bases 1 to 2392)
  AUTHORS   Iwamori,S., Yoshino,S., Ishiwata,K. and Makiguchi,N.
  TITLE     Structure of tyrosine phenol-lyase genes from Citrobacter freundii
            and structural comparison with tryptophanase from Escherichia coli.
  JOURNAL   J. Ferment. Bioeng. 72, 147-151 (1991)
  STANDARD  full staff_review
REFERENCE   2  (bases 1 to 2392)
  AUTHORS   Polak,J.M.
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
REFERENCE   3  (sites)
  AUTHORS   Polak,J.M., Plucienniczak,G., Galas,E. and Brzeski,H.
  JOURNAL   Unpublished (1992)
  STANDARD  full staff_review
COMMENT     *source: strain=NCIB 11490; *source: clone=pICT11;
            From EMBL    entry CFTPLG;  dated 30-JUN-1992.
FEATURES             Location/Qualifiers
     conflict        331
                     /note="replace a"
                     /citation=[1]
     conflict        341
                     /note="replace t"
                     /citation=[1]
     conflict        421
                     /note="replace a"
                     /citation=[1]
     conflict        422
                     /note="replace c"
                     /citation=[1]
     conflict        426
                     /note="replace a"
                     /citation=[1]
     conflict        444
                     /note="replace c"
                     /citation=[1]
     RBS             482..486
     CDS             493..1863
                     /EC_number="4.1.99.2"
                     /product="tyrosine phenol-lyase"
                     /gene="tpl"
                     /codon_start=1
                     /translation="MNYPAEPFRIKSVETVSMIPRDERLKKMQEAGYNTFLLNSKDIY
                     IDLLTDSGTNAMSDKQWAGMMMGDEAYAGSENFYHLERTVQELFGFKHIVPTHQGRGA
                     ENLLSQLAIKPGQYVAGNMYFTTTRYHQEKNGAVFVDIVRDEAHDAGLNIAFKGDIDL
                     KKLQKLIDEKGAENIAYICLAVTVNLAGGQPVSMANMRAVRELTEAHGIKVFYDATRC
                     VENAYFIKEQEQGFENKSIAEIVHEMFSYADGCTMSGKKDCLVNIGGFLCMNDDEMFS
                     SAKELVVVYEGMPSYGGLAGRDMEAMAIGLREAMQYEYIEHRVKQVRYLGDKLKAAGV
                     PIVEPVGGHAVFLDARRFCEHLTQDEFPAQSLAASIYVETGVRSMERGIISAGRNNVT
                     GEHHRPKLETVRLTIPRRVYTYAHMDVVADGIIKLYQHKEDIRGLKFIYEPKQLRFFT
                     ARFDYI"
     conflict        567
                     /note="replace t"
                     /citation=[1]
     conflict        588
                     /note="replace a"
                     /citation=[1]
     conflict        681
                     /note="replace c"
                     /citation=[1]
     conflict        702
                     /note="replace c"
                     /citation=[1]
     conflict        756
                     /note="replace c"
                     /citation=[1]
     conflict        777
                     /note="replace t"
                     /citation=[1]
     conflict        792
                     /note="replace c"
                     /citation=[1]
     conflict        822
                     /note="replace a"
                     /citation=[1]
     conflict        831
                     /note="replace g"
                     /citation=[1]
     conflict        951
                     /note="replace t"
                     /citation=[1]
     conflict        987
                     /note="replace a"
                     /citation=[1]
     conflict        1008
                     /note="replace c"
                     /citation=[1]
     conflict        1020
                     /note="replace c"
                     /citation=[1]
     conflict        1026
                     /note="replace t"
                     /citation=[1]
     conflict        1032
                     /note="replace g"
                     /citation=[1]
     conflict        1035
                     /note="replace a"
                     /citation=[1]
     conflict        1038
                     /note="replace c"
                     /citation=[1]
     conflict        1053
                     /note="replace a"
                     /citation=[1]
     conflict        1059
                     /note="replace g"
                     /citation=[1]
     conflict        1062
                     /note="replace g"
                     /citation=[1]
     conflict        1071
                     /note="replace c"
                     /citation=[1]
     conflict        1082..1083
                     /note="replace atg (MET)"
                     /citation=[1]
     conflict        1086
                     /note="replace c"
                     /citation=[1]
     conflict        1104
                     /note="replace t"
                     /citation=[1]
     conflict        1105..1107
                     /note="replace gca (ALA)"
                     /citation=[1]
     conflict        1110
                     /note="replace a"
                     /citation=[1]
     conflict        1113
                     /note="replace c"
                     /citation=[1]
     conflict        1163..1164
                     /note="replace ttc (PHE)"
                     /citation=[1]
     conflict        1164
                     /note="replace c"
                     /citation=[1]
     conflict        1194
                     /note="replace t"
                     /citation=[1]
     conflict        1248
                     /note="replace t"
                     /citation=[1]
     conflict        1266
                     /note="replace t"
                     /citation=[1]
     conflict        1278
                     /note="replace t"
                     /citation=[1]
     conflict        1341
                     /note="replace t"
                     /citation=[1]
     conflict        1353
                     /note="replace c"
                     /citation=[1]
     conflict        1383
                     /note="replace c"
                     /citation=[1]
     conflict        1386
                     /note="replace c"
                     /citation=[1]
     conflict        1395
                     /note="replace c"
                     /citation=[1]
     conflict        1407
                     /note="replace t"
                     /citation=[1]
     conflict        1413
                     /note="replace c"
                     /citation=[1]
     conflict        1428
                     /note="replace t"
                     /citation=[1]
     conflict        1431
                     /note="replace g"
                     /citation=[1]
     conflict        1434
                     /note="replace c"
                     /citation=[1]
     conflict        1437
                     /note="replace c"
                     /citation=[1]
     conflict        1464
                     /note="replace t"
                     /citation=[1]
     conflict        1473
                     /note="replace c"
                     /citation=[1]
     conflict        1488
                     /note="replace a"
                     /citation=[1]
     conflict        1491
                     /note="replace t"
                     /citation=[1]
     conflict        1512
                     /note="replace g"
                     /citation=[1]
     conflict        1533
                     /note="replace t"
                     /citation=[1]
     conflict        1566
                     /note="replace g"
                     /citation=[1]
     conflict        1569
                     /note="replace c"
                     /citation=[1]
     conflict        1581
                     /note="replace g"
                     /citation=[1]
     conflict        1587
                     /note="replace c"
                     /citation=[1]
     conflict        1599
                     /note="replace t"
                     /citation=[1]
     conflict        1620
                     /note="replace a"
                     /citation=[1]
     conflict        1623
                     /note="replace t"
                     /citation=[1]
     conflict        1683
                     /note="replace g"
                     /citation=[1]
     conflict        1719
                     /note="replace c"
                     /citation=[1]
     conflict        1722
                     /note="replace c"
                     /citation=[1]
     conflict        1731
                     /note="replace t"
                     /citation=[1]
     conflict        1737
                     /note="replace g"
                     /citation=[1]
     conflict        1749
                     /note="replace g"
                     /citation=[1]
     conflict        1828
                     /note="replace c"
                     /citation=[1]
     conflict        1830
                     /note="replace c"
                     /citation=[1]
     conflict        1854
                     /note="replace c"
                     /citation=[1]
     conflict        1857
                     /note="replace t"
                     /citation=[1]
     conflict        1865
                     /note="replace t"
                     /citation=[1]
     conflict        1870
                     /note="replace a"
                     /citation=[1]
     misc_structure  1876..1895
                     /note="termination loop"
     conflict        1884
                     /note="replace t"
                     /citation=[1]
     conflict        1886
                     /note="replace a"
                     /citation=[1]
     conflict        1887
                     /note="replace g"
                     /citation=[1]
     conflict        1901
                     /note="insertion t"
                     /citation=[1]
     conflict        1906
                     /note="replace t"
                     /citation=[1]
     conflict        1925
                     /note="replace g"
                     /citation=[1]
     conflict        1958
                     /note="replace c"
                     /citation=[1]
     conflict        1963
                     /note="replace g"
                     /citation=[1]
     conflict        1964
                     /note="deletion"
                     /citation=[1]
     conflict        1965
                     /note="deletion"
                     /citation=[1]
     conflict        2041
                     /note="deletion"
                     /citation=[1]
BASE COUNT      650 a    523 c    578 g    641 t
ORIGIN
        1 gatctgactc actgaagcaa atagcaaact ctgaaacaac accgttttgt acactttgct
       61 ttacactttt agtgatgtga atcacaaata tcactctgtg aagtgatccg actctcacaa
      121 aatgaggtgt actcattcac caatacatta tgcgcccagc ctggaaagtt atgttttcag
      181 tatttccact ctccgcagac acactacgac gaatgcaaat cgaaaagcaa aaaaaaactt
      241 gttgaatatt aacgggtaaa aaaatgacgt gtgatttgca tcacccacat ttacagcttt
      301 ttaaattatt gccagtgatt aatgttgacc gggctatttc cataatcaat caaatcttgc
      361 atagtgcctc cacattattt ctccccctga ctcaggaggc gaatagttat atttcatcag
      421 gatttgttga tgaaccaggt atgatttact tcacattaat acgtacatga ccactgttac
      481 tggagaaaca aaatgaatta tccggcagaa cccttccgta ttaaaagcgt tgaaactgta
      541 tctatgatcc cgcgtgatga acgcctcaag aaaatgcagg aagcgggtta caatactttc
      601 ctgttaaatt cgaaagatat ttatattgac ctgctgacag acagtggcac taacgcaatg
      661 agcgacaagc agtgggccgg aatgatgatg ggtgatgaag cgtacgcggg cagcgaaaac
      721 ttctatcatc tggaaagaac cgtgcaggaa ctgtttggct ttaaacatat tgttccgact
      781 caccaggggc gtggcgcaga aaacctgtta tcgcagctgg ctattaaacc tgggcaatat
      841 gttgccggga atatgtattt cactaccacc cgttatcacc aggaaaaaaa tggtgcggtg
      901 tttgtcgata tcgttcgtga cgaagcgcac gatgccggtc tgaatattgc gtttaaaggt
      961 gatatcgatc ttaaaaaatt acaaaagctg attgatgaaa aaggcgcaga gaatattgcg
     1021 tatatctgcc ttgcggtgac ggttaacctc gcgggcggcc aaccggtctc gatggctaac
     1081 atgcgtgcgg tgcgtgaact gacagaagcg catggcatta aagtgttcta cgacgctacc
     1141 cgctgcgtag aaaacgccta ctttatcaaa gagcaagagc agggctttga gaacaagagc
     1201 atcgccgaga tcgtgcatga gatgttcagc tacgccgacg gttgtaccat gagtggtaaa
     1261 aaagactgtc tggtgaacat cggcggcttc ctgtgcatga acgatgacga aatgttctct
     1321 tctgccaaag agttagtcgt ggtctacgaa gggatgccat cttacggcgg cctggccgga
     1381 cgtgatatgg aagcgatggc gattggcctg cgtgaagcca tgcagtacga atatattgag
     1441 caccgcgtga agcaggttcg ctacctgggc gataagctga aagccgctgg cgtaccgatt
     1501 gttgaaccgg taggcggtca cgcggtattc ctcgatgcgc gtcgcttctg cgagcatctg
     1561 acgcaagatg agttcccggc acaaagtctg gctgccagca tctatgtgga aaccggcgtg
     1621 cgcagtatgg agcgcggaat tatctctgcg ggccgtaata acgtgaccgg tgaacaccac
     1681 agaccgaaac tggaaaccgt gcgtctgact attccacgtc gtgtttatac ctacgcacat
     1741 atggatgttg tggctgacgg tattattaaa ctttaccagc acaaagaaga tattcgcggg
     1801 ctgaagttta tttacgagcc gaagcagttg cgtttcttta ctgcacgctt tgattacatc
     1861 taaaaaatag ttatggcccc atcacgtgat ggggcttttt gtattccttt tccatgaaca
     1921 ggaaaccttt gcggtgcccg ttaccgtgac ataactaaaa aaaagtaaac cctgactccg
     1981 acatttttat tatccgcatg atgtacaaat gacagctgta attataacga atagtctaca
     2041 aggaacattt atcatggata ttaatgccat tgagcgacag agtagtaccc ccggactgat
     2101 tagcggaacc atgttggtta tcgcgaccgt agtgggtggc gggatgttct ccctgccgat
     2161 tgcgatggcc ggcgtatggt tccccggtgc gtctgttatt cttctgttta tcgccatcat
     2221 gatgttatta accggtctga tgctggtcga agttaacctg cattatggcg ctggtgccag
     2281 ttttaatacc tttactcagg atttattagg caggaagtgg aatatcgtgg ttggtatcgc
     2341 tttcggcttt gtgctgtata ttctgaccta tgcctatatc tccgatgaat tc
//