HDTenEightyP/NetNews huggingface dataset:
From: kristoff@NET.BIO.NET (David Kristofferson)
Newsgroups: bionet.general
Subject: Yet another test
Date: 30 Sep 92 14:43:41 GMT
Distribution: bionet


Checking for duplicate postings.  Some messages may have been
duplicated last night when the residual genbank.bio.net mail queue was
transferred to net.bio.net.  I'm checking to see if this is still
happening.
From: olds@helix.nih.gov (James L. Olds)
Subject: Need advice on the *best* peptide sequencing outfit
Organization: (National Institutes of Health, Bethesda, MD)
Distribution: na
Date: Mon, 5 Oct 1992 20:06:55 GMT

Hi, we are currently in the midst of looking for a place to sequence
a low mol weight protein that we have isolated. Ideally, we're looking
for a place in the Washington DC area that has an absolutely stellar
reputation and is very timely. Money is no object.
Please e-mail me directly with any suggestions and I will summarize
if asked.

--
****************************************************************************
* James L. Olds Ph.D.                 Neural Systems Section               *
* domain:olds@helix.nih.gov           NINDS, NIH, Bethesda, MD. 20892 USA  *
****************************************************************************
Newsgroups: bionet.general,bionet.software
From: gilbertd@sunflower.bio.indiana.edu (Don Gilbert)
Subject: Re: Help with Macintosh Gopher Client(s)
Keywords: help me get it working
Organization: Biology, Indiana University - Bloomington
Distribution: bionet
Date: Wed, 30 Sep 1992 21:41:55 GMT

I can't help you with that gopher client, but you may want to
look at another one (in the same gopher server that you
connected to for Genbank searches): 

GopherApp is an Internet information service program for 
Macintosh computers.  It provides a Finder-like link to the range 
of Internet Gopher services.

You may obtain GopherApp thru anonymous ftp to ftp.bio.indiana.edu, in 
folder /util/gopher, as gopherapp.hqx. You may also obtain updates 
using Gopher to this same IUBio archive (host=ftp.bio.indiana.edu,
port=70). Look for a folder called "IUBio Software+Data/GopherApp".

Comments, bug reports and suggestions for new features
may be addressed via e-mail to

	     Software@Bio.Indiana.Edu
	
History
 
29 Sep 92, v 1.3b60  Only change over previous release is addition of
	display of text files with greater than 32,000 characters.  What a simple
	addition, and the Marvels of MacApp/OOP let me fold in this change from
	SeqApp into GopherApp with just one compile.
	

-- 
Don Gilbert                                     gilbert@bio.indiana.edu
biocomputing office, biology dept., indiana univ., bloomington, in 47405
Newsgroups: bionet.general
From: harper@convex.csc.FI (Rob Harper)
Subject: Re: Explanatory books (FAQ)
Organization: Finnish Academic and Research Network Project - FUNET
Distribution: bionet
Date: Mon, 5 Oct 92 07:39:52 GMT

In <4016@fcs280s.ncifcrf.gov> pnh@fcsparc6.ncifcrf.gov (Paul N Hengen) writes:

I would like to share this idea with you regarding the preparation of
FAQ's. It is something I have done here at CSC on our gopher.

When people respond to you via e-mail if you have a e-mail reader such as
ELM you can tag all the messages regarding hybridization and then save them
into a ELM mail folder called hybrid. Do the same for PCR or any other
subject that you want to make a FAQ from and save them all into different 
ELM folders.

If you pop down to the Mail directory you will see that the ELM folder is
in actual fact a file. You can copy this file into the the gopher directory
and when you look at it with gopher the Subject lines from the individual
mail messages appear as an ITEM on the gopher menu... which is very neat.

So instead of having ONE big file to scroll through you have small individual
items to sellect from and you can further use the / command within gopher
to search for specific words within the folder which is a quick way to
to get to the subjects that you want.

I have one directory in our gopher called "Essential reading for the novice
Bionaut". When I read Usenet and I find an article that has good information
in it I mail a copy of it to myself. Read it in ELM, tag it and save it to
the "esential" mail folder and it can then be read from within gopher. I am
also using this method to make up a FAQ on "biological FTP sites"

I would be interested in ALSO receiving any responses that you get from
your survey so if you forward them on to me I would willingly make up
a gopher FAQ from them since it is so easy to do.

RGDS -=ROB=- 
--
 ~ Rob Harper                    ~ E-mail:          harper@convex.csc.fi    
 ~ Finnish State Computer Centre ~ Molbio/software: harper@nic.funet.fi
 ~ P.O. Box 40                   ~ Telephone:       +358 0 457 2076
 ~ SF-02101 Espoo Finland        ~ Fax:             +358 0 457 2302
Newsgroups: bionet.general
From: AJB@DLVH.DARESBURY.AC.UK
Subject: DARESBURY email server alive
Original-To: BIOFORUM@UK.AC.DARESBURY
Distribution: bionet
Date: Tue, 6 Oct 1992 20:53:00 GMT

Email subscribers to Daresbury will already have realised the server is
alive after being deluged with a 3-day backlog of BIOSCI messages. This
message is essentially to inform NEWS subscribers that the complete BIOSCI
audience is once again there.

Alan Bleasby
SEQNET/Biosci manager
SERC Daresbury Laboratory
From: robert@arbo.microbiol.uwa.oz.au (Robert Coelen)
Newsgroups: bionet.general
Subject: GenBank farewell
Date: 30 Sep 1992 04:02:19 GMT
Organization: University of Western Australia
NNTP-Posting-Host: mve.microbiol.uwa.edu.au

From the land Down Under
to all of you at genbank.bio.net




WELL DONE   !!!!



Robert Coelen
on behalf of the internet citizens of AU !!!
From: g5000005@nickel.laurentian.ca
Newsgroups: bionet.general
Subject: Wanted: Statistica 3.1 manual
Date: 30 Sep 92 19:08:18 GMT
Organization: Laurentian University

Hello netters!

	I am looking for a manual/book for the statistical analysis program 
called Statistica 3.1 (for DOS). I am currently using VAX/VMS SPSSx but I will
be graduating soon (what time is it now?) and have to decide between SPSS-PC+ 
and Statistica for my PC.

	If anyone has used this package I would be interested to hear your 
comments/criticisms regarding this package.

	Thanks in advance!

Norm
From: bdest+@pitt.edu (Benoit Desjardins)
Newsgroups: bionet.general
Subject: information request: biomathematics / mathematical biology
Date: 6 Oct 92 05:22:55 GMT
Organization: University of Pittsburgh

I would like to know what are the good research centers (for
graduate training and postdocs) in biomathematics (mathematical
biology).  Can someone provide me with this information? Also, does
anyone know about the research activities of Oxford's Center for
Mathematical Biology?

                                       Thank you

                                       B. Desjardins
                                       benoit@med.pitt.edu

P.S. Please reply to "benoit@med.pitt.edu"
From: ADAMS_W@GOLD.COLORADO.EDU
Newsgroups: bionet.general
Subject: Formation of carotenoid newsgroup
Date: 2 Oct 92 20:52:00 GMT
Distribution: bionet

To Whom it May Concern,
	I should like to place my vote in favor of forming a
carotenoid newsgroup, to be headed by Pablo Scolnik.  I 
vote YES.
	Sincerely yours,  William W. Adams III
From: pnh@fcsparc6.ncifcrf.gov (Paul N Hengen)
Newsgroups: bionet.general
Subject: Re: Explanatory books (FAQ)
Summary: Please contribute
Date: 2 Oct 92 14:53:01 GMT
Distribution: bionet
Organization: Frederick Cancer Research and Development Center

I've started the construction of a FAQ list for
bionet.molbio.methds-reagnts and would like other people to
contribute to it's growth and evolution. Please feel free
to include what you like and any suggestions for a better
FAQ list. Thank You. -Paul.

Paul N. Hengen
National Cancer Institute
Frederick Cancer Research and Development Center
Frederick, Maryland 21702-1201 USA
voice: (301) 846-5581
FAX: (301) 846-5598
e-mail: pnh@ncifcrf.gov

--------------------------------------------------------------------------------
I think a simple format of questions and answers is a good start since I
haven't seen or heard of a FAQ list at all for this newgroup. Perhaps for
simplicity, I should ask for an ASCII format of not more than 80 characters
in line length. Please include references if possible. What do you think an
appropriate reference format should be? They can be provided as J. Bacteriol.,
Medlars, BiBTeX, etc.. If the list grows too large, it may need to be separated
into catagories such as  1) General information 2) PCR technology
3) Hybridization 4) Plasmids ..etc. 


The FAQ list could be in the form:

.........+.........+.........+.........+.........+.........+.........+.........+
        10        20        30        40        50        60        70        80


This is the FAQ list prototype for bionet.molbio.methds-reagnts
Last update was on 01 October 1992


[Note: Please add any relevant or missing information and return to me at
the internet address pnh@ncifcrf.gov]

================================================================================

What are some good reference books for methods in molecular biology?

There are many books which provide details and recipes for molecular
cloning techniques. The following is a list of reference books which
are commonly available...

Maniatis, T., E. F. Fritsch, and J. Sambrook. 1982. Molecular cloning: A
laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor,
New York.

Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular Cloning: A
laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor,
New York.

Miller, J. H. 1972. Experiments in molecular genetics, p.431-435. Cold
Spring Harbor Laboratory, Cold Spring Harbor, New York.
 
Ausubel, F. M., R. Brent, R. E. Kingston, D. D. Moore, J. G. Seeidman,
J. A. Smith, and K. Struhl. 1991. Current Protocols in Molecular Biology.
John Wiley and Sons, New York.



Please add to this list and return to me by e-mail. Thank You.
From: IAE@CU.NIH.GOV ("Irene Anne Eckstrand")
Newsgroups: bionet.general
Subject: (none)
Date: 30 Sep 92 11:50:58 GMT
Distribution: bionet

Now that the GenBank contract has ended, I want to take
this opportunity to publically thank the folks at IG
(especially Mike Kelly, Dave Kristofferson, and Julie
Lawrence), LANL (especially Paul Gilna, Michael Cinkosky,
and Christian Burks), and the GenBank Advisors (too
numerous to name here) for their contributions to making
GenBank a success.  While it is not perfect or even as
useful as we dream it could be, GenBank has made great
strides in providing a valuable resource to the scientific
community.  I am constantly amazed at the amount of work
and dedication it takes to keep GenBank up and running;
fortunately, there have been many people who contribute
both.  Thank you all.

And thanks, too, to the critics of GenBank for keeping us
on our toes and helping to make GenBank even more useful.

irene eckstrand
GenBank Project Officer
National Institute of General Medical Sciences
National Institutes of Health
From: scostant@cc.curtin.edu.au
Subject: Mouse Immunglobulin Allotypes?
Organization: Curtin University of Technology
Date: Sun, 27 Sep 1992 11:03:30 GMT



I am working with the following strains of mice; AKR, C57Bl/6J, CBA/H and BALB/c
I would like to know what the immunoglobulin gene allotypes are for these 
strains. ie. allotypes for gammaG1, gammaG2a, and gammaG2b?  The names of 
relavent journals would be appreciated. I have only been able to find the 
gamma2a allotype for the BALB/c mice but am looking for the others.

Thanks in advance,
Paul Costantino (Ph D student)
Curtin University of Technology
Perth, Western Austarlia

email (scostant@cc.curtin.edu.au)

Xref: sparky bionet.general:1758 bionet.software:1864
From: axa12@po.cwru.edu (Ashok Aiyar)
Newsgroups: bionet.general,bionet.software
Subject: Help with Macintosh Gopher Client(s)
Summary: Mac Gopher client
Keywords: help me get it working
Date: 30 Sep 92 20:15:32 GMT
Distribution: bionet
Organization: Department of Biochemistry, CWRU Medical School
NNTP-Posting-Host: meds38943.bioc.cwru.edu


Hello:

I am trying to get the Macintosh Gopher client working for
one of our users.

I FTPed the Mac client from boombox.micro.umn.edu (I tried
both the existing Mac Client and the beta-test Gopher+ client).

The user in my department has a Mac SE that is on our campus
TCP/IP network (running System 6.07).

We cannot get the Gopher client to work.  The Gopher+ client
does work and we can connect to ftp.bio.indiana.edu (port 70).

He can view and save all text-files.  However when he tries to
view and save Genbank sequence files that were obtained by searching 
with a keyword, he finds that he cannot view or save those files.
When we try to look at a sequence file, we get a little dialog box
with a an [OK] button in the middle.  Clicking on the button
does nothing.

When we view other text files, there is no problem in viewing or
saving them.

Please help.

Ashok
----------------------------------------------------------------------
                             Ashok A. Aiyar
                       Department of Biochemistry
                         CWRU School of Medicine
----------------------------------------------------------------------
From: ajb@gserv1.dl.ac.uk (Alan Bleasby)
Newsgroups: bionet.general
Subject: DARESBURY email server down
Date: 3 Oct 92 23:16:06 GMT
Distribution: bionet
Organization: SERC Daresbury Laboratory, Warrington, UK

The email server covering Europe, Africa & Asia is currently down. No
BIOSCI messages will be lost ... they are being queued on another
machine.

The problem does not affect USENET News.

Obviously, when European/Asian/African email subscribers receive this message
the problem has been solved.

Alan Bleasby
SEQNET/BIOSCI manager
SERC Daresbury Laboratory
Newsgroups: bionet.general
From: aarman@magnus.acs.ohio-state.edu (Ahmet Arman)
Subject: Rickettsia belli DNA Library
Organization: The Ohio State University
Date: Mon, 5 Oct 1992 01:13:46 GMT
From: JLOIJENS@MACC.WISC.EDU (Joost Loijens)
Newsgroups: bionet.general
Subject: Explanatory books
Date: 29 Sep 92 18:49:00 GMT
Distribution: bionet


     I was flipping through the September issue to Trends in Biochemical
Science over lunch today.  I noticed a book review of "Recombinant DNA" by
J. D. Watson and a few others (I'm not sure if it was a second edition).
According to the reviewer it was a easy to read and had references for
further reading in each section (for the grad. student as the article states).
I'm not sure if it is available yet but it might be what you are looking for.
If it is any good maybe you could tell us about it in a post...
 
A fellow grad. student,
 
 
  Joost Loijens
 
  Program in Cell and Molecular Biology     internet: jloijens@macc.wisc.edu
  University of Wisconsin-Madison           bitnet  : jloijens@wiscmac
From: kristoff@NET.BIO.NET (Dave Kristofferson)
Newsgroups: bionet.general
Subject: Hello from net.bio.net!
Date: 30 Sep 92 05:41:11 GMT
Distribution: bionet


So far, so good.  This is coming from the new net.bio.net machine.

Dave Kristofferson
From: kristoff@NET.BIO.NET (Dave Kristofferson)
Newsgroups: bionet.general
Subject: Re: biosci WAIS server down?
Date: 2 Oct 92 20:59:23 GMT
Distribution: bionet

> I think the biosci WAIS server may be down.  It is a very useful resource that
> is badly missed when it goes down.  Thanks.
> 
> 
> Keith
> 
> --
> Keith L. Steward                          Voice: 519 679-2111 ext. 6618
> Dept. Microbiology & Immunology             Fax: 519 661-3499
> Dental Sciences Bldg.                  Internet: KLSTEWARD@UWO.CA
> University of Western Ontario                    
> London, Ontario, Canada, N6A 5C1
> 

It is down temporarily as we transfer our services over to the new
machine.  It should be back on-line on net.bio.net instead of
genbank.bio.net soon.

				Sincerely,

				Dave Kristofferson
				BIOSCI/bionet Manager

				kristoff@net.bio.net
From: IAE@CU.NIH.GOV ("Irene Anne Eckstrand")
Newsgroups: bionet.general
Subject: (none)
Date: 5 Oct 92 16:03:02 GMT
Distribution: bionet

I would like the advice of scientists on the net regarding
some writing I am doing about the merits of basic research
support at NIH.  While most of us understand that products,
medical applications, etc. have their roots firmly embedded
in basic research, it is good to document this now and then.
Turns out now is a good time.

I am looking for good examples of biotechnologies and medical
applications (therapies, mapped genes,...) that are RECENT and
IMPORTANT and can be traced to basic research.  Your favorite
example would be gratefully appreciated!!

Irene Anne Eckstrand, Ph.D.
National Institute of General Medical Sciences
NIH
iae@cu.nih.gov
Tel: 301-496-7137
FAX: 301-402-3665
From: kristoff@GENBANK.BIO.NET (Dave Kristofferson)
Newsgroups: bionet.general
Subject: Re: GenBank Farewell
Date: 29 Sep 92 20:18:15 GMT
Distribution: bionet


Jim,

	I don't think that this will make it into an anthology of
English verse 8-), but thanks anyway!

	Less than four hours left for GOS e-mail server operation!

				Sincerely,

				Dave Kristofferson
				GenBank Manager for one last day

				kristoff@genbank.bio.net
From: kristoff@net.bio.net (David Kristofferson)
Newsgroups: bionet.general
Subject: Re: Formation of carotenoid newsgroup
Date: 3 Oct 92 18:18:35 GMT
Distribution: bionet
Organization: BIOSCI International Newsgroups for Biology

Everyone,

Please don't post further comments about this newsgroup or votes to
the BIOFORUM/bionet.general newsgroup.  Only votes sent to
biovote@net.bio.net or biovote@daresbury.ac.uk are counted, and
discussion is closed on any proposed newsgroup once voting commences.

				Sincerely,

				Dave Kristofferson
				BIOSCI/bionet Manager

				kristoff@net.bio.net
From: kristoff@NCBI.NLM.NIH.GOV (Dave Kristofferson)
Newsgroups: bionet.general
Subject: Another test
Date: 30 Sep 92 06:03:02 GMT
Distribution: bionet


Test of old bioforum@genbank.bio.net address.  New address is
bioforum@net.bio.net.
From: kh@litsun17.epfl.ch (Mark S.)
Newsgroups: bionet.general
Subject: Re: antibody fluorescence
Date: 28 Sep 92 21:06:08 GMT
Reply-To: kh@litsun.epfl.ch
Distribution: bionet
Organization: Ecole Polytechnique Federale de Lausanne
In-reply-to: wmelchior@NTBTOX.NCTR.FDA.GOV's message of 23 Sep 92 13:04:08 GMT


Trp fluorescence is dependent on the micro environment. Going from an aqueous surrounding (as in solution) to a more hydrophobic one (as in bound to lipids or within a protein surrounding there are two measurble changes: the fluorescence quantum yield goes up, probably because the exited state looses a raditionless relaxation mechanism thru contact with water molecules. Moreover there is a blue shift in the emision (250 -> 220 nm, depending on hydrophobicity), I think because the exited state is polar and 




has a higher energy in a hydrophobic environment.

If you want to use quenchers: there are a lot of them, both aqueous and hydrophobic. 


-------------------------------------------------------------------------
Mark Soekarjo				Living in a postcard

postcards to 	la Maison Rose		e-mail to	kh@litsun.epfl.ch
		Ch. des Clos 53		fax		+41'21'693'41'11
		CH-1024 Ecublens	voice		+41'21'693'37'02
		****europe*****
--
-------------------------------------------------------------------------
Mark Soekarjo				Living in a postcard

postcards to 	la Maison Rose		e-mail to	kh@litsun.epfl.ch
		Ch. des Clos 53		fax		+41'21'693'41'11
		CH-1024 Ecublens	voice		+41'21'693'37'02
		****europe*****
From: DEMMIGADAM_B@GOLD.COLORADO.EDU
Newsgroups: bionet.general
Subject: Creation of carotenoid newsgroup
Date: 2 Oct 92 20:42:00 GMT
Distribution: bionet

To whom it may concern,
	I should like to register a YES vote in favor of creating
a carotenoid newsgroup.
	Sincerely yours,
		Barbara Demmig-Adams
Xref: sparky bionet.general:1762 bionet.software:1872
From: axa12@po.cwru.edu (Ashok Aiyar)
Newsgroups: bionet.general,bionet.software
Subject: Re: Help with Macintosh Gopher Client(s)
Keywords: help me get it working
Date: 1 Oct 92 16:10:49 GMT
Distribution: bionet
Organization: Department of Biochemistry, CWRU Medical School
NNTP-Posting-Host: meds38943.bioc.cwru.edu

In article <BvEuxw.G0q@usenet.ucs.indiana.edu> gilbertd@sunflower.bio.indiana.edu (Don Gilbert) writes:

>I can't help you with that gopher client, but you may want to
>look at another one (in the same gopher server that you
>connected to for Genbank searches): 
>
>GopherApp is an Internet information service program for 
>Macintosh computers.  It provides a Finder-like link to the range 
>of Internet Gopher services.
>
>You may obtain GopherApp thru anonymous ftp to ftp.bio.indiana.edu, in 
>folder /util/gopher, as gopherapp.hqx. You may also obtain updates 
>using Gopher to this same IUBio archive (host=ftp.bio.indiana.edu,
>port=70). Look for a folder called "IUBio Software+Data/GopherApp".

Thank you, I didn't need a HyperCard based application.  Your
Gopher Client works beautifully.

Ashok
----------------------------------------------------------------------
                             Ashok A. Aiyar
                       Department of Biochemistry
                         CWRU School of Medicine
----------------------------------------------------------------------
From: kristoff@net.bio.net (David Kristofferson)
Newsgroups: bionet.general
Subject: Re: Creation of carotenoid newsgroup
Date: 3 Oct 92 18:15:26 GMT
Distribution: bionet
Organization: BIOSCI International Newsgroups for Biology

DEMMIGADAM_B@GOLD.COLORADO.EDU writes:

>To whom it may concern,
>	I should like to register a YES vote in favor of creating
>a carotenoid newsgroup.
>	Sincerely yours,
>		Barbara Demmig-Adams

Votes must be mailed to biovote@net.bio.net or biovote@daresbury.ac.uk
in order to be counted.

				Sincerely,

				Dave Kristofferson
				BIOSCI/bionet Manager

				kristoff@net.bio.net
From: kh@litsun17.epfl.ch (Mark S.)
Newsgroups: bionet.xtallography
Subject: neophyte
Date: 6 Oct 92 09:43:42 GMT
Reply-To: kh@litsun.epfl.ch
Distribution: bionet
Organization: Ecole Polytechnique Federale de Lausanne


i want to try crystallizing a membrane protein, but have no clue how to...

Any good books to read?
Hints?

thanx

-------------------------------------------------------------------------
Mark Soekarjo				Living in a postcard

postcards to 	la Maison Rose		e-mail to	kh@litsun.epfl.ch
		Ch. des Clos 53		fax		+41'21'693'41'11
		CH-1024 Ecublens	voice		+41'21'693'37'02
		****europe*****
--
-------------------------------------------------------------------------
Mark Soekarjo				Living in a postcard

postcards to 	la Maison Rose		e-mail to	kh@litsun.epfl.ch
		Ch. des Clos 53		fax		+41'21'693'41'11
		CH-1024 Ecublens	voice		+41'21'693'37'02
		****europe*****
From: bl275@cleveland.Freenet.Edu (Dan Diaz)
Newsgroups: bionet.xtallography
Subject: My crystals came and went
Date: 2 Oct 1992 01:27:46 GMT
Organization: Case Western Reserve University, Cleveland, OH (USA)
Reply-To: bl275@cleveland.Freenet.Edu (Dan Diaz)
NNTP-Posting-Host: hela.ins.cwru.edu


I set up a number of dialysis buttons in an attempt to crystallize
exonuclease VII from E. coli.  After a few weeks in the cold, I observed a
single cystal in one button sitting in ammonium sulfate.  After a few weeks
the crystal disappeared.  I am in the process of setting up some more
crystallizations.  What happened?
-- 
Dizzy Dan         ddiaz@cwru.bitnet      bl275@cleveland.freenet.edu
Dept. Biochemistry & Wine Tasting  CWRU School of Medicine & Sushi
"I usually like to live in the past, but my time machine is broken"
From: satish@a.chem.upenn.edu (Satish Nair)
Newsgroups: bionet.xtallography
Subject: PROTEIN question.
Date: 30 Sep 92 19:29:59 GMT
Distribution: bionet.xtallography
Organization: University of Pennsylvania

Hi,

I have a question regarding W. Steigmann's PROTEIN package.  I am using
the package for data scaling, merging and reduction. I've noticed that
the program only accepts structure factors (and not intensities) for 
data handling. Does this mean that the data reduction statistics that the
program produces are based on Fs and not Is?  Or does the program internally
convert structure factors to intensities for statistics purposes?  

Thanks,

Satish Nair