From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: SKC-2=streptokinase [Streptococcus equisimilis, group C, ATCC 9542,
Date: 31 Dec 92 01:48:13 GMT
Distribution: bionet
Checksum: 23124 4

LOCUS       S115306      1245 bp    DNA             BCT       18-DEC-1992
DEFINITION  SKC-2=streptokinase [Streptococcus equisimilis, group C, ATCC 9542,
            Genomic, 1245 nt]
ACCESSION   S46536
KEYWORDS    .
SOURCE      Streptococcus equisimilis group C ATCC 9542
  ORGANISM  Streptococcus equisimilis
            Unclassified.
REFERENCE   1  (bases 1 to 1245)
  AUTHORS   Estrada,M.P., Hernandez,L., Perez,A., Rodriguez,P., Serrano,R.,
            Rubiera,R., Pedraza,A., Padron,G., Antuch,W., de la Fuente,J. and
            et,al.
  TITLE     High level expression of streptokinase in Escherichia coli.
  JOURNAL   Biotechnology (N.Y.) 10, 1138-1142 (1992)
  STANDARD  full automatic
COMMENT     This entry [NCBI gibbsq 115306] was created by the journal scanning
            component of NCBI/GenBank at the National Library of Medicine.
            This sequence comes from Fig. 2A.
FEATURES             Location/Qualifiers
     CDS             1..1245
                     /note="Mature protein lacks initial Met.  streptokinase;
                     For the protein sequence (NCBI gibbsq 115308):  Method:
                     conceptual translation supplied by author.  This sequence
                     comes from Fig. 2B."
                     /product="SKC-2"
                     /codon_start=1
                     /translation="IAGPEWLLDRPSVNNSQLVVSVAGTVEGTNQDISLKFFEIDLTS
                     RPAHGGKTEQGLSPKSKPFATDSGAMPHKLEKADLLKAIQEQLIANVHSNDDYFEVID
                     FASDATITDRNGKVYFADKDGSVTLPTQPVQEFLLSGHVRVRPYKEKPIQNQAKSVDV
                     EYTVQFTPLNPDDDFRPGLKDTKLLKTLAIGDTITSQELLAQAQSILNKTHPGYTIYE
                     RDSSIVTHDNDIFRTILPMDQEFTYHVKNREQAYEINKKSGLNEEINNTDLISEKYYV
                     LKKGEKPYDPFDRSHLKLFTIKYVDVNTNELLKSEQLLTASERNLDFRDLYDPRDKAK
                     LLYNNLDAFGIMDYTLTGKVEDNHDDTNRIITVYMGKRPEGENASYHLAYDKDRYTEE
                     EREVYSYLRYTGTPIPDNPNDK"
BASE COUNT      428 a    271 c    234 g    312 t
ORIGIN      
        1 attgctggac ctgagtggct gctagaccgt ccatctgtca acaacagcca attagttgtt
       61 agcgttgctg gtactgttga ggggacgaat caagacatta gtcttaaatt ttttgaaatt
      121 gacctaacat cacgacctgc tcatggagga aagacagagc aaggcttaag tccaaaatca
      181 aaaccatttg ctactgatag tggcgcgatg ccacataaac ttgaaaaagc tgacttacta
      241 aaggctattc aagaacaatt gatcgctaac gtccacagta acgacgacta ctttgaggtc
      301 attgattttg caagcgatgc aaccattact gatcgaaacg gcaaggtcta ctttgctgac
      361 aaagatggtt cggtaacctt gccgacccaa cctgtccaag aatttttgct aagcggacat
      421 gtgcgcgtta gaccatataa agaaaaacca atacaaaatc aagcgaaatc tgttgatgtg
      481 gaatatactg tacagtttac tcccttaaac cctgatgacg atttcagacc aggtctcaaa
      541 gatactaagc tattgaaaac actagctatc ggtgacacca tcacatctca agaattacta
      601 gctcaagcac aaagcatttt aaacaaaacc cacccaggct atacgattta tgaacgtgac
      661 tcctcaatcg tcactcatga caatgacatt ttccgtacga ttttaccaat ggatcaagag
      721 tttacttacc atgtcaaaaa tcgggaacaa gcttatgaga tcaataaaaa atctggtctg
      781 aatgaagaaa taaacaacac tgacctgatc tctgagaaat attacgtcct taaaaaaggg
      841 gaaaagccgt atgatccctt tgatcgcagt cacttgaaac tgttcaccat caaatacgtt
      901 gatgtcaaca ccaacgaatt gctaaaaagc gagcagctct taacagctag cgaacgtaac
      961 ttagacttca gagatttata cgatcctcgt gataaggcta aactactcta caacaatctc
     1021 gatgcttttg gtattatgga ctatacctta actggaaaag tagaggataa tcacgatgac
     1081 accaaccgta tcataaccgt ttatatgggc aagcgacccg aaggagagaa tgctagctat
     1141 catttagcct atgataaaga tcgttatacc gaagaagaac gagaagttta cagctacctg
     1201 cgttatacag ggacacctat acctgataac cctaacgaca aataa
//