HDTenEightyP/NetNews huggingface dataset:
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Rhesus macaque polyoma virus agnoprotein gene, 5' end.
Date: 22 Aug 92 10:01:31 GMT
Distribution: bionet
Checksum: 12500     3

LOCUS       SV4AGP1       395 bp ds-DNA             VRL       22-AUG-1992
DEFINITION  Rhesus macaque polyoma virus agnoprotein gene, 5' end.
ACCESSION   M99348
KEYWORDS    agnoprotein; enhancer.
SOURCE      Rhesus macaque polyomavirus (strain Br124) DNA.
  ORGANISM  Rhesus macaque polyomavirus
            Viridae; ds-DNA nonenveloped viruses; Papovaviridae;
            Polyomaviruses.
REFERENCE   1  (sites)
  AUTHORS   van Heuverswyn,H. and Fiers,W.
  TITLE     Nucleotide sequence of the Hind-C fragment of Simian Virus 40 DNA:
            comparison of the 5'-untranslated region of wild-type virus and of
            some deletion mutants
  JOURNAL   Eur. J. Biochem. 100, 51-60 (1979)
  STANDARD  full staff_review
REFERENCE   2  (bases 1 to 395)
  AUTHORS   Ilyinskii,P.O., Daniel,M.D., Horvath,C. and Desrosiers,R.C.
  TITLE     Genetic analysis of SV40 from brain and kidney of macaque monkeys
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     enhancer        complement(170..241)
                     /note="72-bp repeat"
     variation       replace(complement(241^242),
                     "ggtgtggaaagtccccaggctccccagcaggcagaagtatgcaaagcatgcatctca
                     attagtcagcaacca")
                     /note="2nd 72bp repeat (exists in HV40 strain 776,
                     nucleotides 179-250)"
                     /citation=[1]
     CDS             326..395
                     /note="partial"
                     /product="agnoprotein"
                     /codon_start=1
                     /translation="MVLRRLSRQASVKVRRSWTESKK"
                     /partial
BASE COUNT       96 a     98 c    113 g     88 t
ORIGIN
        1 caaaagccta ggcctccaaa aaagcctcct cactacttct ggaatagctc agaggccgag
       61 gcggcctcgg cctctgcata aataaaaaaa attagtcagc catggggcgg agaatgggcg
      121 gaactgggcg gagttagggg cgggatgggc ggagttaggg gcgggactat ggttgctgac
      181 taattgagat gcatgctttg catacttctg cctgctgggg agcctgggga ctttccacac
      241 cctaactgac acacattcca cagctggttc tttccgcctc agaaggtacc taaccaagtt
      301 cctctttcag aggttatttc aggccatggt gctgcgccgg ctgtcacgcc aggcctccgt
      361 taaggttcgt aggtcatgga ctgaaagtaa aaaaa
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Stereum complicatum ribosomal RNA (62LR3) large subunit, partial.
Date: 19 Aug 92 10:03:54 GMT
Distribution: bionet
Checksum: 18356     2

LOCUS       STQRRLS       230 bp ss-rRNA            RNA       19-AUG-1992
DEFINITION  Stereum complicatum ribosomal RNA (62LR3) large subunit, partial.
ACCESSION   M98607
KEYWORDS    large subunit ribosomal RNA.
SOURCE      Stereum complicatum (library: Duke culture collection 783) cDNA to
            rRNA.
  ORGANISM  Stereum complicatum
            Eukaryota; Fungi; Amastigomycota; Basidiomycetes.
REFERENCE   1  (bases 1 to 230)
  AUTHORS   Hibbett,D.S. and Vilgalys,R.
  TITLE     Phylogenetic relationships of the basidiomycete genus Lentinus
            inferred from molecular and morphological characters
  JOURNAL   Unpublished (1992)
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     rRNA            <1..>230
                     /note="Aligns to region between positions 371 to 597
                     inSaccharomyces cerevisiae large subunit nuclear rRNA."
                     /product="large subunit ribosomal RNA"
                     /citation=[1]
BASE COUNT       50 a     50 c     74 g     56 t
ORIGIN
        1 gatgaaagca ctttgggaag agagttaaac gtacgtgatt gttgaaaggg aaacgcttaa
       61 gtcagtcgcg tcggccggga ctcagccttg atccgcttgg tgtactttcc ggtcgacggg
      121 ccagcatcag ttttgaccgc gagataaggc ggagggaatg tggctctctc gggagtttta
      181 tagcccttcg tcagatgtcg cggttgggac tgaggaactc agcacgcctt
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Brugia malayi antigen mRNA.
Date: 18 Aug 92 10:00:28 GMT
Distribution: bionet
Checksum: 30417     2

LOCUS       BRPSERANT     718 bp ss-mRNA            INV       18-AUG-1992
DEFINITION  Brugia malayi antigen mRNA.
ACCESSION   M98813
KEYWORDS    antigen.
SOURCE      Brugia malayi cDNA to mRNA.
  ORGANISM  Brugia malayi
            Eukaryota; Animalia; Eumetazoa; Nematoda; Secernentea; Spiruria;
            Spirurida; Spirurina; Filarioidea; Gnathostomatidae.
REFERENCE   1  (bases 1 to 718)
  AUTHORS   Dissanayake,S., Min,X. and Piessens,W.F.
  TITLE     A cloned antigen for serological diagnosis of Wuchereria bancrofti
            microfilaremia with day time blood
  JOURNAL   Unpublished (1992)
  STANDARD  simple staff_entry
BASE COUNT      258 a    133 c    122 g    205 t
ORIGIN
        1 gaattccggg tcacttcatc actcaatcta acaaaaatga aatattttat tttcctttca
       61 atcggtctga tagcagcagc atcagctcaa agagaagcac aattacctca gccagaaata
      121 cctccatttt tgtccggtgt acccagccat gttgtgaagc aattttttga tctactcaaa
      181 gctgatgaat caaaaactga cccgcagact gaagcagata tcgaagcatt cattcgcaga
      241 cttggtggcg attaccaaac tcgatttgag caattcaagc aagagataaa gaaagaaaaa
      301 gctcagtatg agaaaatcca tcaagcagca ttattaaaat tttcaccagc agcaagagaa
      361 gctgatgcca agatgtctgc tattgccgac agtacgcaac tgaccaatca ccagaaaacg
      421 gaacagatca aagcaattat ggattcatta agcgaagctg ttcgaaagga aatcttggaa
      481 ggatttaatt cacaataaag tgttatatca gtgctgctga gtattacaat taagattttt
      541 tcaaaatgaa agaagaatca tatatgcgac aaaaagtaat aaatagataa gaaaatattg
      601 ttgtcatcaa tgtggaatat ttcaagctaa ttaattatta cacatctgtt atctttgtta
      661 tttcattttt atcctcatta attattgcta ttaaagttct catgctagcc cggaattc
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Homo sapiens testicular protein (TSPY) mRNA, complete cds.
Date: 14 Aug 92 10:00:44 GMT
Distribution: bionet
Checksum: 57829     3

LOCUS       HUMRNATSPY   1075 bp ss-mRNA            PRI       14-AUG-1992
DEFINITION  Homo sapiens testicular protein (TSPY) mRNA, complete cds.
ACCESSION   M98525
KEYWORDS    testicular protein.
SOURCE      Homo sapiens male adult testis cDNA to mRNA.
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 1075)
  AUTHORS   Zhang,J.S., Yang-Feng,T.L., Muller,U., Mohandas,T., de Jong,P.J.
            and Lau,Y.-F.C.
  TITLE     Molecular isolation and characterization of an expressed gene from
            the human Y chromosome
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     mRNA            1..1075
     CDS             74..835
                     /product="testicular protein"
                     /codon_start=1
                     /translation="MEAVQEGAAGVESEQAALGEEAVLLLDDIMAEVEVVAEEEGLVE
                     RREEAQPRQQAVPGPGPMTPESALEELLAVQVELEPVNAQARKAFSRQREKMERRRKP
                     HLDRRGAVIQSVPGFWANVIANHPQMSALITDEDEDMLSYMVSLEVEEEKHPVHLCKI
                     MLFFRSNPYFQNKVITKEYLVNITEYRASHSTPIEWYPDYEVEAYRRRHHNSSLNFFN
                     WFSDHNFAGSNKIAESPDRSYVRTCGAIPCNTTRG"
BASE COUNT      278 a    249 c    336 g    212 t
ORIGIN
        1 gggtttctgt ggcgtgggtc gggcagcaca ggccttggtg tgtgcgagtg ccaaggaggg
       61 caccgccttc aggatggagg ctgtgcagga gggggcggcc ggggtggaga gtgagcaggc
      121 ggctttgggg gaggaggcgg tgctgctgtt ggatgacata atggcggagg tggaggtggt
      181 ggcggaggag gagggcctcg tggagcggcg ggaggaggcc cagccccgac agcaggctgt
      241 gcctggccct gggcccatga ccccagagtc tgcactggag gagctgctgg ccgttcaggt
      301 ggagctggag ccggttaatg cccaagccag gaaggccttt tctcggcagc gggaaaagat
      361 ggagcggagg cgcaagcccc acctagaccg cagaggcgcc gtcatccaga gcgtccctgg
      421 cttctgggcc aatgttattg caaaccaccc ccagatgtca gccctgatca ctgacgaaga
      481 tgaagacatg ctgagctaca tggtcagcct ggaggtggaa gaagagaagc atcctgttca
      541 tctctgcaag atcatgttgt tctttcggag taacccctac ttccagaata aagtgattac
      601 caaggaatat ctggtgaaca tcacagaata cagggcttct cattccactc caattgagtg
      661 gtatccggat tatgaagtgg aggcctatcg ccgcagacac cacaacagca gccttaactt
      721 cttcaactgg ttctctgacc acaacttcgc aggatctaac aagattgctg agtcccctga
      781 cagatcctat gtaaggacct gtggcgcaat cccctgcaat actacaagag gatgaagcca
      841 cctgaagagg gaacagagac gtcaggggac tcccagttgt tgagttgaat atgatggagc
      901 atcagatttt acctaataca gcagaactcc taaaaagtta cagccatatg caggacggca
      961 gtactcagca tggtcttatg cacaggaact aaaggaaaaa gagatcgagt cacaaaaatt
     1021 caggaagagg gggtaaatgt ggattgtatg gaatgaaaaa taaacattct caagg
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human chromosome 4 sequence-tagged site STS4-389
Date: 21 Aug 92 10:09:44 GMT
Distribution: bionet
Checksum: 20898     3

LOCUS       HUM4STS389    432 bp ds-DNA             PRI       21-AUG-1992
DEFINITION  Human chromosome 4 sequence-tagged site STS4-389
ACCESSION   L00832
KEYWORDS    STS; human chromosome 4; sequence tagged site.
SOURCE      Homo sapiens, clone p4-412 from a chromosome 4 plasmid library;
            vector pBluescript II KS + (Stratagene)
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 432)
  AUTHORS   Goold,R.D., diSibio,G., Dugaiczyk,A., Smith,K.A., Xu,H.,
            Magrane,G., Lang,D.B., Cox,D.R., Masters,S.B. and Myers,R.M.
  TITLE     Sequence-Tagged Sites for Human Chromosome 4
  JOURNAL   Unpublished (1992) see COMMENT for author address
  STANDARD  full automatic
COMMENT     PCR components: 25 ng of human genomic DNA, 10 pmol of each
            oligonucleotide, 200 micro-M dNTPs, 0.25 U Taq  polymerase (Cetus)
            in 10 micro-l of 50 mM KCl-20 mM  Tris-HCl, pH 8.3 (at room temp),
            2.5 mM MgCl-2.  Initial denaturation at 94degC for 1.5 min, then 30
            cycles of  94degC for 15 sec, 62degC for 23 sec, and 72degC for 30
            sec, followed by a final extension at 72degC for 3.5 min, using a
            Perkin-Elmer 9600 thermocycler. PCR-amplified product size 270 bp.
            Sequence submitted by:
            Human Genome Mapping Center
            Box 0925
            University of California San Francisco
            San Francisco, CA 94143-0925  USA
            Phone: (415) 502-1612   Fax: (415) 476-8391
            e-mail: hgmcprobes@cgl.ucsf.edu
FEATURES             Location/Qualifiers
     misc_feature    13..50
                     /label=Primer1
     misc_feature    complement(268..293)
                     /label=Primer2
BASE COUNT      110 a     86 c     86 g    142 t      8 others
ORIGIN
        1 gatctttttt atctgaacag aatgagttaa aagaattaat tctgtaaatt cagtgaaaca
       61 atatccagct ttgtccacaa ctcctttttc taaatgagtg tcttcatatc agattcctca
      121 gggagagagt cagattactg caagtgtatc tgattctgat tttaaagaag gttctggaat
      181 gattccattt gtcctaagaa tgcaccagaa taaaatgatt tcttatattc aggcagnatt
      241 tttaaaattc taagtagctt ttgcttatcc cacttattta gtcctcctgt tgagcgtaga
      301 tttggagtac attgtnggag cccctctaat gtgctttgnt gctaacaggn tgncacnccc
      361 acccatgaga agattgctca gngcactggg gtcctccttg ttctcccggg ttcctccctg
      421 tggnggggga cc
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human chromosome 4 sequence-tagged site STS4-126
Date: 21 Aug 92 10:06:45 GMT
Distribution: bionet
Checksum: 49509     3

LOCUS       HUM4STS126    354 bp ds-DNA             PRI       21-AUG-1992
DEFINITION  Human chromosome 4 sequence-tagged site STS4-126
ACCESSION   M95279
KEYWORDS    STS; human chromosome 4; sequence tagged site.
SOURCE      Homo sapiens, clone C4-86 from Los Alamos National Laboratory
            chromosome 4 cosmid library, plate 4-1-1R, pos8C; vector SuperCos 1
            (Stratagene)
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 354)
  AUTHORS   Goold,R.D., diSibio,G., Dugaiczyk,A., Smith,K.A., Xu,H.,
            Magrane,G., Lang,D.B., Cox,D.R., Masters,S.B. and Myers,R.M.
  TITLE     Sequence-Tagged Sites for Human Chromosome 4
  JOURNAL   Unpublished (1992) see COMMENT for author address
  STANDARD  full automatic
COMMENT     PCR components: 25 ng of human genomic DNA, 10 pmol of each
            oligonucleotide, 200 micro-M dNTPs, 0.25 U Taq  polymerase (Cetus)
            in 10 micro-l of 50 mM KCl-20 mM  Tris-HCl, pH 8.3 (at room temp),
            2.5 mM MgCl-2.  Initial denaturation at 94degC for 1.5 min, then 30
            cycles of  94degC for 15 sec, 64degC for 23 sec, and 72degC for 30
            sec, followed by a final extension at 72degC for 3.5 min, using a
            Perkin-Elmer 9600 thermocycler. PCR-amplified product size 277 bp.
            Sequence submitted by:
            Human Genome Mapping Center
            Box 0925
            University of California San Francisco
            San Francisco, CA 94143-0925  USA
            Phone: (415) 502-1612   Fax: (415) 476-8391
            e-mail: hgmcprobes@cgl.ucsf.edu
FEATURES             Location/Qualifiers
     misc_feature    23..43
                     /label=Primer1
     misc_feature    complement(278..299)
                     /label=Primer2
BASE COUNT      103 a     84 c     81 g     81 t      5 others
ORIGIN
        1 angaaggtat gccattattt tatgaagctg gtggaagggc ctccccacct ccacgtgagg
       61 cacccaagat ggcgaagtca cccataccaa agagtatnta ttcagaagct tcaacactgg
      121 accatcactc acactatgat ggggctgtga taacggaaga tcacttagtc taggaaagaa
      181 ctcctccatg tgccatcata accactgtag ggagcaggca agtcactcaa cttttcagat
      241 actgctgtcc atgtctgtaa gatggagcca agactaccta cctatccgtg tgctgtgagc
      301 accgaaggag acgatgcana ttnctttgtt aatgatgtng cacaaaagga tgac
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human chromosome 4 sequence-tagged site STS4-16
Date: 21 Aug 92 10:07:09 GMT
Distribution: bionet
Checksum: 36498     3

LOCUS       HUM4STS16     466 bp ds-DNA             PRI       21-AUG-1992
DEFINITION  Human chromosome 4 sequence-tagged site STS4-16
ACCESSION   M95316
KEYWORDS    STS; human chromosome 4; sequence tagged site.
SOURCE      Homo sapiens, clone L4-6 from the C25 lambda library (Pritchard,
            Genomics 4, 408, 1989); vector - Lambda Dash II (Stratagene)
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 466)
  AUTHORS   Goold,R.D., diSibio,G., Dugaiczyk,A., Smith,K.A., Xu,H.,
            Magrane,G., Lang,D.B., Cox,D.R., Masters,S.B. and Myers,R.M.
  TITLE     Sequence-Tagged Sites for Human Chromosome 4
  JOURNAL   Unpublished (1992) see COMMENT for author address
  STANDARD  full automatic
COMMENT     PCR components: 25 ng of human genomic DNA, 40 pmol of each
            oligonucleotide, 200 micro-M dNTPs, 0.25 U Taq  polymerase (Cetus)
            in 10 micro-l of 50 mM KCl-20 mM  Tris-HCl, pH 8.3 (at room temp),
            2.5 mM MgCl-2.  Initial denaturation at 94degC for 1.5 min, then 30
            cycles of  94degC for 15 sec, 63degC for 23 sec, and 72degC for 30
            sec, followed by a final extension at 72degC for 3.5 min, using a
            Perkin-Elmer 9600 thermocycler. PCR-amplified product size 466 bp.
            Sequence submitted by:
            Human Genome Mapping Center
            Box 0925
            University of California San Francisco
            San Francisco, CA 94143-0925  USA
            Phone: (415) 502-1612   Fax: (415) 476-8391
            e-mail: hgmcprobes@cgl.ucsf.edu
FEATURES             Location/Qualifiers
     misc_feature    1..25
                     /label=Primer1
     misc_feature    complement(442..466)
                     /label=Primer2
BASE COUNT      149 a     82 c     79 g    156 t
ORIGIN
        1 gaattctttc cagaggtttt tctgtttacc ttgttcagat ccttcagagg aatccctatt
       61 atatggcagg tatatgatat gtatttctta aacaataaac ttgaaagtcc aaaattactc
      121 cttgatccat ggactgcaga ataaatgtta ttttagcctg tcagaaaaac aatactaact
      181 tgcatatgtt catcagagcc ttggtgacca ggtgtattgc caataagcag taatattttg
      241 agaggaatct tgttttcaat gcagtaggtc tcaacagtgc acttaaaata ttcagtatta
      301 cataacgtaa acaaatgtcc tgtcattcga tctttgttgt tccatttata gagcatacgc
      361 aaagtacatt ttgtataatt cttaagagcc tagggtttca gaatggtaaa tgagcattga
      421 cttcaactta aagtaaccag ctgcattaac cttcaacaag agtcat
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Proteobacteria 16S rRNA (PSB_1)
Date: 15 Aug 92 10:02:56 GMT
Distribution: bionet
Checksum: 37987     2

LOCUS       PRTS16SRNA    158 bp ss-RNA             BCT       15-AUG-1992
DEFINITION  Proteobacteria 16S rRNA (PSB_1)
ACCESSION   X59161
KEYWORDS    16S ribosomal RNA homologue; ribosomal RNA.
SOURCE      Proteobacterium sp. RNA.
  ORGANISM  Proteobacterium sp.
            Prokaryotae; Eubacteriomycetes; Eubacteriales.
REFERENCE   1  (bases 1 to 158)
  AUTHORS   Busse,H.J., El Banna,T., Oyaizu,H. and Auling,G.
  TITLE     Identification of xenobiotic degrading isolates from the
            beta-subclass of Proteobacteria by a polyphasic approach including
            16S rRNA partial sequencing
  JOURNAL   Int. J. Syst. Bacteriol. 42, 19-26 (1992)
  STANDARD  full automatic
COMMENT     *source: strain=PSB_1 (PSB-1);
            From EMBL    entry PPS16SRNA;  dated 13-MAR-1992.
FEATURES             Location/Qualifiers
     rRNA            <1..>158
                     /note="16S rRNA"
BASE COUNT       39 a     42 c     48 g     29 t
ORIGIN
        1 gggcttcaca cgtcatacaa tggtcgggac agagggttgc caacccgcga gggggagcca
       61 atctcagaaa ccccatcgta gtccggattg caggctgcaa ctcgcctgca tgaagtcgga
      121 atcgctagta atcgcggatc agcatgtcgc ggtgaata
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: M.musculus parvalbumin gene exon 4 3'-part
Date: 22 Aug 92 10:01:16 GMT
Distribution: bionet
Checksum: 38950     2

LOCUS       MUSPVAEX42     32 bp ds-DNA             ROD       22-AUG-1992
DEFINITION  M.musculus parvalbumin gene exon 4 3'-part
ACCESSION   X62832
KEYWORDS    cytosolic calcium binding protein; parvalbumin; pva gene.
SOURCE      Mus musculus DNA.
  ORGANISM  Mus musculus
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Rodentia; Myomorpha; Muridae; Murinae.
REFERENCE   1  (bases 1 to 32)
  AUTHORS   Schleef,M.
  JOURNAL   Unpublished (1991)
  STANDARD  simple staff_entry
REFERENCE   2  (sites)
  AUTHORS   Schleef,M., Zuehlke,C.H., Jockusch,H. and Schoeffl,F.
  TITLE     The structure of the mouse parvalbumin gene
  JOURNAL   Mamm. Genome 3, 217-225 (1992)
  STANDARD  full staff_review
COMMENT     *source: strain=ssp. domesticus, strain=A2G; *source: stage=adult;
            *source: chromosome=15;
            From EMBL    entry MMPVAEX42;  dated 01-JUL-1992.
FEATURES             Location/Qualifiers
     prim_transcript <1..>32
                     /usedin=X62826:pva_transcr
     exon            <1..12
                     /number=4
                     /usedin=X62826:pva_mRNA
                     /usedin=X62827:pva_CDS
                     /note="3'end"
                     /gene="pva"
     intron          13..>32
                     /number=4
                     /note="5'end"
BASE COUNT        7 a      4 c     12 g      9 t
ORIGIN
        1 ttggggttga aggtgagtag ccgtgactct aa
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human dopamine transporter mRNA, partial cds.
Date: 14 Aug 92 10:00:32 GMT
Distribution: bionet
Checksum: 16209     2

LOCUS       HUMDOPATRA    278 bp ss-mRNA            PRI       14-AUG-1992
DEFINITION  Human dopamine transporter mRNA, partial cds.
ACCESSION   M96670
KEYWORDS    dopamine transporter.
SOURCE      Homo sapiens Substantia Nigra cDNA to mRNA.
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 278)
  AUTHORS   Bannon,M.J., Poosch,M.S., Xia,Y., Goebel,D.J., Cassin,B. and
            Kapatos,G.
  TITLE     Dopamine transporter mRNA content in human substantia nigra
            decreases precipitously with age
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 89, 7095-7099 (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     CDS             1..278
                     /product="dopamine transporter"
                     /note="putative"
                     /codon_start=2
                     /translation="LHYLFSSFTTELPWIHCNNSWNSPNCSDAHPGDSSGDSSGLNDT
                     FGTTPAAEYFERGVLHLHQSHGIDDLGPPRWQLTACLVLVIVLLYFSL"
                     /partial
BASE COUNT       48 a    102 c     68 g     60 t
ORIGIN
        1 gctgcactat ctcttctcct ccttcaccac ggagctcccc tggatccact gcaacaactc
       61 ctggaacagc cccaactgct cggatgccca tcctggtgac tccagtggag acagctcggg
      121 cctcaacgac acttttggga ccacacctgc tgccgagtac tttgaacgtg gcgtgctgca
      181 cctccaccag agccatggca tcgacgacct ggggcctccg cggtggcagc tcacagcctg
      241 cctggtgctg gtcatcgtgc tgctctactt cagcctct
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human chromosome 4 sequence-tagged site STS4-92
Date: 21 Aug 92 10:11:19 GMT
Distribution: bionet
Checksum: 39187     3

LOCUS       HUM4STS92     422 bp ds-DNA             PRI       21-AUG-1992
DEFINITION  Human chromosome 4 sequence-tagged site STS4-92
ACCESSION   M95239
KEYWORDS    STS; human chromosome 4; sequence tagged site.
SOURCE      Homo sapiens, clone C4-83 from Los Alamos National Laboratory
            chromosome 4 cosmid library, plate 4-1-1R, pos 5C; vector SuperCos
            1 (Stratagene)
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 422)
  AUTHORS   Goold,R.D., diSibio,G., Dugaiczyk,A., Smith,K.A., Xu,H.,
            Magrane,G., Lang,D.B., Cox,D.R., Masters,S.B. and Myers,R.M.
  TITLE     Sequence-Tagged Sites for Human Chromosome 4
  JOURNAL   Unpublished (1992) see COMMENT for author address
  STANDARD  full automatic
COMMENT     PCR components: 25 ng of human genomic DNA, 10 pmol of each
            oligonucleotide, 200 micro-M dNTPs, 0.25 U Taq  polymerase (Cetus)
            in 10 micro-l of 50 mM KCl-20 mM  Tris-HCl, pH 8.3 (at room temp),
            2.5 mM MgCl-2.  Initial denaturation at 94degC for 1.5 min, then 30
            cycles of  94degC for 15 sec, 62degC for 23 sec, and 72degC for 30
            sec, followed by a final extension at 72degC for 3.5 min, using a
            Perkin-Elmer 9600 thermocycler. PCR-amplified product size 255 bp.
            Sequence submitted by:
            Human Genome Mapping Center
            Box 0925
            University of California San Francisco
            San Francisco, CA 94143-0925  USA
            Phone: (415) 502-1612   Fax: (415) 476-8391
            e-mail: hgmcprobes@cgl.ucsf.edu
FEATURES             Location/Qualifiers
     misc_feature    10..32
                     /label=Primer1
     misc_feature    complement(240..264)
                     /label=Primer2
BASE COUNT      114 a     81 c     63 g    155 t      9 others
ORIGIN
        1 ttaagagggg tggagacttt gcactggtga tgtttgtctt tgaacagtca aatactctat
       61 gatgtcattt ggatatgtat catttcttgc acttgttttn ggccttgaat catttnctat
      121 tgtggaggct aatntaaatg tgtagaaaag aataatatgc tcagccttat taatctacac
      181 aacatgtagt gtcttattca gattattttt attaattctt caaaggnccc tctgcatcat
      241 gtcaaacccc atgataattt ctccctttcc ccaagtcgnt tttataatga attcagagtt
      301 cagcatccca tgcgtgttta cacactttta cataaacctt atctgatatn gttatgcata
      361 tctaaatctt tccataaatg attcttacct aatatgncat cttanatntg ggcctcccaa
      421 cc
//