From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: L.esculentum gene for polyphenol oxidase.
Date: 25 Jul 92 10:07:01 GMT
Distribution: bionet
Checksum: 65369     6

LOCUS       TOMPOOXA     2213 bp ds-DNA             PLN       25-JUL-1992
DEFINITION  L.esculentum gene for polyphenol oxidase.
ACCESSION   Z12833
KEYWORDS    polyphenol oxidase.
SOURCE      Lycopersicon esculentum DNA.
  ORGANISM  Lycopersicon esculentum
            Eukaryota; Plantae; Embryobionta; Magnoliophyta; Magnoliopsida;
            Asteridae; Solanales; Solanaceae.
REFERENCE   1  (bases 1 to 2212)
  AUTHORS   Newman,S.M., Eannetta,N.T., Yu,H., Prince,J.P., de Vicente,C.,
            Tanksley,S.D. and Steffens,J.C.
  TITLE     Organization of the tomato polyphenol oxidase gene family
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
REFERENCE   2  (bases 1 to 2213)
  AUTHORS   Newman,S.M.
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
COMMENT     *source: strain=cv. VFNT cherry; *source: clone_library=Charon 35
            library of R. Fischer; *source: clone=TGPPO 6; *source: chromosome=
            8; *source: is_macronuclear=N; *source: is_proviral=N; *source:
            is_germline=N;
            From EMBL    entry LEPOOXA;  dated 26-JUN-1992.
FEATURES             Location/Qualifiers
     CAAT_signal     5..8
     TATA_signal     103..107
     CDS             151..2043
                     /product="polyphenol oxidase precursor"
                     /codon_start=1
                     /translation="MASLCSNSSSTSLKTPFTSSTTCLSSTPTASQLFLHGKRNKTFK
                     VSCKVTNTNGNQDETNSVDRRNVLLGLGGLYGVANAIPLAASAAPTPPPDLSSCNKPK
                     INATTEVPYFCCAPKPDDMSKVPYYKFPSVTKLRIRPPAHALDEAYIAKYNLAISRMK
                     DLDKTQPDNPIGFKQQANIHCAYCNGGYSIDGKVLQVHNSWLFFPFHRWYLYFYERIL
                     GSLIDDPTFGLPFWNWDHPKGMRFPPMFDVPGTALYDERRGDQIHNGNGIDLGYFGDQ
                     VETTQLQLMTNNLTLMYRQLVTNSPCPLMSLVDLTLFGSTVEDAGTVENIPHSPVHIW
                     VGTRRGSVLPVGKISNGEDMGNFYSAGLDPLFYCHHSNVDRMWNEWKATGGKRTDIQN
                     KDWLNSEFFFYDENGNPFKVRVRDCLDTKKMGYDYHATATPWRNFKPKTKASAGKVNT
                     GSIPPESQVFPLAKLDKAISFSINRPASSRTQQEKNAQEEVLTFNAIKYDNRDYIRFD
                     VFLNVDNNVNANELDKAEFAGSYTSLPHVHRVGDPKHTATATLRLAITELLEDIGLED
                     EDTIAVTLVPKKGDISIGGVEIKLAIVKLVCVVNLLTLQLNKDRFCYDSVFVCWFVCL
                     FFNFHV"
     transit_peptide 151..408
     mat_peptide     409..2040
                     /product="polyphenol oxidase"
     misc_binding    742..805
                     /bound_moiety="copper, tyrosinase-like CuA bindingsite"
     misc_binding    1132..1290
                     /bound_moiety="copper, tyrosinase-like CuB bindingsite"
BASE COUNT      646 a    427 c    456 g    684 t
ORIGIN
        1 ttcccaatta ataaatatga atttatcgat taaataatat ctttataaaa taataaagct
       61 tatttgaaat ggagtaatat tttatttggc cccaacacat tatataaggc aatgtatagc
      121 cctatgaatc ttcatccaaa gctagcaata atggcaagtt tgtgtagtaa tagtagtagt
      181 acatctctca aaactccttt tacttcttcc accacttgtt tatcttccac tccaacggct
      241 tctcaacttt tcctacatgg aaaacgtaac aaaacattca aagtttcatg caaggttacc
      301 aatactaacg gtaaccaaga tgaaacgaat tctgttgatc gaagaaatgt tcttcttggc
      361 ttaggtggtc tttatggtgt tgctaatgct ataccattag ctgcatccgc tgctcctaca
      421 ccacctcctg atctctcatc ttgtaataaa ccaaagatta atgcaactac ggaggtgcca
      481 tacttttgtt gcgcgcctaa gcctgatgat atgtccaaag ttccttatta caagttccct
      541 tctgtgacta agctccgtat tcgtccccct gctcatgctc ttgatgaggc gtatattgcc
      601 aagtacaatc tggcgattag ccgaatgaaa gatcttgata agacacaacc tgataaccct
      661 attggtttta agcaacaagc taatatacat tgtgcttatt gtaatggtgg ttattctatt
      721 gatggcaaag tgttgcaagt tcataactcg tggcttttct tcccgttcca tagatggtac
      781 ttgtacttct acgaaagaat cttgggatca ctcatcgatg atccaacatt cggtttgcca
      841 ttttggaatt gggatcatcc aaagggcatg cgtttccctc ccatgtttga tgttccaggg
      901 accgcccttt atgacgaaag gcgtggtgat caaatccata atgggaacgg gatagatctt
      961 ggttattttg gggatcaagt tgagacaact cagttgcagt tgatgactaa taacttaacc
     1021 ctgatgtacc gtcagttggt aactaattct ccatgtcctc ttatgtcttt ggtggacctt
     1081 acactctttg ggagtacggt cgaagacgcg ggaactgttg aaaacatccc tcacagccct
     1141 gttcacattt gggttggtac gagacgaggt tcagttttac ctgtaggtaa aatatcaaat
     1201 ggtgaggata tgggtaattt ctattcagct ggtttagacc cgcttttcta ttgccatcac
     1261 agcaatgtgg acaggatgtg gaacgaatgg aaggcaactg gaggtaaacg aacagatata
     1321 caaaacaaag attggttgaa ctcggagttc tttttctatg atgagaatgg aaatccgttc
     1381 aaagtgagag tcagagactg tttggatacg aagaagatgg ggtatgatta ccacgcaacg
     1441 gcgaccccat ggcgtaactt caagccaaaa acaaaggctt cagctgggaa agtgaatacg
     1501 ggttcaattc ctcccgaaag ccaggtattc ccattggcta aactcgacaa agcaatttca
     1561 ttttccatca acaggcctgc ttcctcgagg actcaacaag agaaaaatgc acaagaggag
     1621 gtcctaacat tcaatgcaat aaaatatgat aacagagatt acataagatt cgatgtgttc
     1681 ctcaacgtgg ataacaatgt gaacgcgaat gagcttgaca aggcagagtt tgcggggagt
     1741 tatactagct tgccacatgt acacagagtt ggagatccta aacatactgc aactgctact
     1801 ctccggctgg cgataacaga actgttggag gacattggtt tggaagatga agatactatc
     1861 gcggtgactc tggtaccaaa gaaaggtgat atctccattg gaggtgtgga gatcaagctt
     1921 gcgattgtta aattagtgtg tgtagtgaat ctgctgacac tacagttgaa caaggatagg
     1981 ttctgttatg attctgtttt tgtttgttgg tttgtctgtt tgttttttaa tttccatgtt
     2041 tgaaacttga tttccttgta cttgttattc accaataaat gaatccattt tccaacttgt
     2101 tatttgtaac tataaaacaa acttaatatc cacttcgttt tatctcatac aagaaaatgt
     2161 tcaacatgtt gtttattgga atgagtcaaa tgttatgatt tgatgaattt cag
//