From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: B.stearothermophilus insertion sequence IS5376
Date: 4 Sep 92 10:02:30 GMT
Distribution: bionet
Checksum: 43525     5

LOCUS       BACIS5376    2107 bp ds-DNA             BCT       04-SEP-1992
DEFINITION  B.stearothermophilus insertion sequence IS5376
ACCESSION   X67861
KEYWORDS    transposable element.
SOURCE      Bacillus stearothermophilus DNA.
  ORGANISM  Bacillus stearothermophilus
            Prokaryotae; Firmicutes; Endospore-forming rods and cocci;
            Bacillaceae.
REFERENCE   1  (bases 1 to 2107)
  AUTHORS   Xu,K.Y., He,Z.Q., Mao,Y.M., Shen,R.Q. and Sheng,Z.J.
  TITLE     On two transposable elements from bacillus stearothermophilus
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
COMMENT     *source: strain=CU21;
            From EMBL    entry BSIS5376;  dated 13-AUG-1992.
FEATURES             Location/Qualifiers
     repeat_region   1..50
                     /rpt_type=inverted
     RBS             79..85
     CDS             93..1295
                     /note="ORF A"
                     /codon_start=1
                     /translation="MITRGEFFMIKEMYERGMSISDIARELGIDRKTVRKYIHSPNPP
                     SKSKRKQRKSKLDPFKPYLQKRMLEDGVFNSEKLFFEIRQQGYTGGKTILKDYMKPFR
                     ETAKKKYTVRYETLPGEQMQVDWKEVGEVVIEGKKVKLSLFVATLGYSRMKYAVFTTS
                     QDQEHLMECLIQSFKYFGGVPKKVLFDNMKTVTDGREQGVVKWNQRFSEFASYYGFIP
                     KVCRPYRAQTKGKVERAIQYIMDHFYVGTAFESIEELNFLLHRWLDQVANRKPNATTG
                     ISPQERWAEESLKPLPLKDYDTSYLSYRKVHWDGSFSYKGEQWLLSAEYAGKEILVKE
                     RLNGDIRLYFRGEEISHVDQQKKVISFAEKIKKKQTEMAATISPVSVEVDTRPLSVYD
                     AFLRGESS"
     CDS             1292..2047
                     /note="ORF B"
                     /codon_start=1
                     /translation="MKERIHEYCHRLHLPVMAERWSAMAEYASTHNISYSEFLFRLLE
                     AEIVEKQARSIQTLIKLSKLPYRKTIDTFDFTAQPSVDERRIRELLTLSFIDRKENIL
                     FLGPPGIGKTHLAISIGMEAIARGYKTYFITAHDLVNQLRRADQEGKLEKKLRVFVKP
                     TVLIIDEMGYLKLDPNSAHYLFQVIARRYEHAPIILTSNKSFGEWGEIVGDSVLATAM
                     LDRLLHHSIIFNLKGESYRLREKRLQEEKQKDQ"
     repeat_region   2058..2107
                     /rpt_type=inverted
BASE COUNT      650 a    420 c    524 g    513 t
ORIGIN
        1 tgttaaagcc gatgataaaa tccccaatat agccggaata aaattcccca cttacaatag
       61 aaccatagtg tcaacgagag gagctatggt tcatgattac gagaggggaa ttttttatga
      121 tcaaagagat gtatgaaagg ggaatgagta tttccgatat tgcgagggaa ttggggatcg
      181 atcggaaaac cgtccgaaaa tatattcact cccccaatcc tccttccaaa tccaagcgaa
      241 aacaaagaaa aagcaagtta gatccattta agccgtatct tcaaaaacga atgttagaag
      301 atggggtgtt taatagcgaa aagttgtttt ttgaaattcg acaacagggc tatacgggag
      361 gaaagacgat tttaaaagac tatatgaaac ctttccgaga gacggcgaaa aagaaataca
      421 ccgttcgtta tgaaacgctt cctggcgaac aaatgcaagt cgattggaaa gaagttgggg
      481 aggtcgtgat cgaagggaaa aaagtcaagt tatcgctatt tgtggccacg ttaggctatt
      541 cgcggatgaa atacgcggta tttacgacca gccaggatca ggaacactta atggaatgcc
      601 tgattcagag cttcaagtac tttggcgggg ttccgaagaa ggtgttattt gacaatatga
      661 agaccgttac agacgggcga gaacaaggag tggtgaaatg gaatcaacga ttttccgaat
      721 ttgcgagtta ctatggattt attccaaaag tatgccggcc ttaccgggcc cagacaaagg
      781 gaaaagtcga acgagccatt cagtatatca tggatcactt ctatgtgggg acagcgtttg
      841 aaagcatcga ggaattaaat ttccttctcc atcgttggct cgatcaagtg gcgaatcgga
      901 agccaaacgc cactaccggt atttctccgc aagagcgttg ggccgaggag tcactcaagc
      961 ctcttccgtt gaaagattac gatacgagct atctttccta tcggaaggtg cattgggatg
     1021 gcagtttctc ctacaaaggg gaacaatggc tcttatcggc ggagtatgcg ggcaaagaaa
     1081 ttctggtgaa ggagcgatta aatggagata ttcgattgta ctttcgaggg gaggagattt
     1141 ctcacgtgga ccaacagaaa aaagtgattt cattcgccga aaaaataaaa aagaaacaaa
     1201 cggaaatggc cgccaccatt tcgcctgttt cggtggaagt ggatactcgt ccattgtccg
     1261 tttatgacgc attcctgcga ggggaaagct catgaaagaa cgaatacacg agtattgcca
     1321 ccgactccat ttgcctgtca tggcggaacg atggtccgcc atggcagaat acgcctctac
     1381 tcataatata tcatattcag agtttttatt ccgcttatta gaggcagaaa tcgtcgaaaa
     1441 acaggcacga tcgatccaaa cgctcatcaa gctgtccaaa ctgccgtatc gcaagacgat
     1501 cgatacgttt gattttaccg cgcagccttc ggtggatgag cgccggattc gagaactgct
     1561 tacgttgtcc tttattgacc ggaaagaaaa tatcctcttt ctcggtccac cgggtattgg
     1621 gaagacacat ctggcaattt cgattggaat ggaggcgatc gcaagaggat ataaaacgta
     1681 ttttattacc gctcacgatt tggtcaatca gttaagaaga gccgaccagg aaggaaagtt
     1741 ggagaaaaag cttcgtgtct ttgtgaagcc aaccgttctc attattgatg aaatggggta
     1801 tctaaaactg gacccgaaca gcgctcatta cttatttcaa gtgatcgccc ggcggtacga
     1861 gcatgccccg attatcctca cctccaacaa aagctttggg gaatggggag aaatcgtggg
     1921 agactcggtt ttggcgacag cgatgttaga tcgattactg catcattcca tcattttcaa
     1981 cctaaagggg gaaagctatc gattacggga aaagaggctc caagaagaaa aacagaagga
     2041 tcaatgaaag gtccttctgg ggaattttaa accggcgatt ttggggaaaa aataatcggc
     2101 cttgaca
//