From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Transposon Tn1331 (from K.pneumoniae) tnpR, aac, aadA, bla, and
Date: 7 Apr 92 10:06:44 GMT
Distribution: bionet
Checksum: 44399     9

LOCUS       TRNTAAB      3933 bp ds-DNA             BCT       07-APR-1992
DEFINITION  Transposon Tn1331 (from K.pneumoniae) tnpR, aac, aadA, bla, and
            TEM-bla genes.
ACCESSION   M55547 J03325
KEYWORDS    adenylyl transferase; aminoglycosides resistance; beta-lactamase;
            resistance; resolvase.
SOURCE      Transposon Tn1331 DNA.
  ORGANISM  Transposon Tn1331
            Prokaryota; Bacteria.
REFERENCE   1  (bases 1 to 3933)
  AUTHORS   Tolmasky,M.E.
  TITLE     Sequencing and expression of aadA, bla, and tnpR from the
            multiresistance Transposon Tn1331
  JOURNAL   Plasmid 24, 218-226 (1990)
  STANDARD  full staff_entry
FEATURES             Location/Qualifiers
     CDS             53..610
                     /gene="tnpR"
                     /label=ORF1
                     /codon_start=1
                     /translation="MRIFGYARVSTSQQSLDIQIRALKDAGVKANRIFTDKASGSSTD
                     REGLDLLRMKVEEGDVILVKKLDRLGRDTADMIQLIKEFDAQGVAVRFIDDGISTDGD
                     MGQMVVTILSAVAQAERRRILERTNEGRQEAKLKGIKFGRRRTVDRNVVLTLHQKGTG
                     ATEIAHQLSIARSTVYKILEDERAS"
     CDS             793..1398
                     /gene="aac"
                     /label=ORF2
                     /codon_start=1
                     /translation="MSIQHFQTKLGITKYSIVTNSNDSVTLRLMTEHDLAMLYEWLNR
                     SHIVEWWGGEEARPTLADVQEQYLPSVLAQESVTPYIAMLNGEPIGYAQSYVALGSGD
                     GWWEEETDPGVRGIDQLLANASQLGKGLGTKLVRALVELLFNDPEVTKIQTDPSPSNL
                     RAIRCYEKAGFERQGTVTTPDGPAVYMVQTRQAFERTRSVA"
     CDS             1466..2254
                     /gene="aadA"
                     /label=ORF3
                     /codon_start=1
                     /translation="MREAVIAKVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
     CDS             2299..3138
                     /gene="bla"
                     /label=ORF4
                     /codon_start=1
                     /translation="MKDTLMKKILLLHMLVFVSATLPISSVASDEVETLKCTIIADAI
                     TGNTLYETGECARRVSPCSSFKLPLAIMGFDSGILQSPKSPTWELKPEYNPSPRDRTY
                     KQVYPALWQSDSVVWFSQQLTSRLGVDRFTEYVKKFEYGNQDVSGDSGKHNGLTQSWL
                     MSSLTISPKEQIQFLLRFVAHKLPVSEAAYDMAYATIPQYQAAEGWAVHGKSGSGWLR
                     DNNGKINESRPQGWFVGWAEKNGRQVVFARLEIGKEKSDIPGGSKAREDILVELPVLM
                     GNK"
     CDS             3338..3655
                     /gene="tnpR"
                     /label=ORF5
                     /codon_start=1
                     /translation="KEFDAQGVAVRFIDDGISTDGDMGQMVVTILSAVAQAERRRILE
                     RTNEGRQEAKLKGIKFGRRRTVDRNVVLTLHQKGTGATEIAHQLSIARSTVYKILEDE
                     RAS"
                     /partial
     CDS             3838..3933
                     /gene="TEM-bla"
                     /label=ORF6
                     /codon_start=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVK"
                     /partial
BASE COUNT     1040 a    904 c   1052 g    937 t
ORIGIN
        1 ttttggcata atagacacat cgtgtctgat attcgattta aggtacattt ttatgcgaat
       61 ttttggttat gcgcgggtct caaccagcca gcagtccctc gatattcaga tcagagcgct
      121 caaagatgca ggggtaaaag ctaaccgcat ctttaccgac aaggcatccg gcagttcaac
      181 agatcgggaa gggctggatt tgctgaggat gaaggtggag gaaggtgatg tcattctggt
      241 gaagaagctc gaccgtcttg gccgcgacac cgccgacatg atccaactga taaaagagtt
      301 tgatgctcag ggtgtagcgg ttcggtttat tgacgacggg atcagtaccg acggtgatat
      361 ggggcaaatg gtggtcacca tcctgtcggc tgtggcacag gctgaacgcc ggaggatcct
      421 agagcgcacg aatgagggcc gacaggaagc aaagctgaaa ggaatcaaat ttggccgcag
      481 gcgtaccgtg gacaggaacg tcgtgctgac gcttcatcag aagggcactg gtgcaacgga
      541 aattgctcat cagctcagta ttgcccgctc cacggtttat aaaattcttg aagacgaaag
      601 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
      661 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
      721 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
      781 aaaaggaaga gtatgagtat tcaacatttc caaacaaagt taggcatcac aaagtacagc
      841 atcgtgacca acagcaacga ttccgtcaca ctgcgcctca tgactgagca tgaccttgcg
      901 atgctctatg agtggctaaa tcgatctcat atcgtcgagt ggtggggcgg agaagaagca
      961 cgcccgacac ttgctgacgt acaggaacag tacttgccaa gcgttttagc gcaagagtcc
     1021 gtcactccat acattgcaat gctgaatgga gagccgattg ggtatgccca gtcgtacgtt
     1081 gctcttggaa gcggggacgg atggtgggaa gaagaaaccg atccaggagt acgcggaata
     1141 gaccagttac tggcgaatgc atcacaactg ggcaaaggct tgggaaccaa gctggttcga
     1201 gctctggttg agttgctgtt caatgatccc gaggtcacca agatccaaac ggacccgtcg
     1261 ccgagcaact tgcgagcgat ccgatgctac gagaaagcgg ggtttgagag gcaaggtacc
     1321 gtaaccaccc cagatggtcc agccgtgtac atggttcaaa cacgccaggc attcgagcga
     1381 acacgcagtg ttgcctaacc cttccatcga gggggacgtc caagggctgg cgccttggcc
     1441 gccctcatgt caaacgttaa acatcatgag ggaagcggtg atcgcgaaag tatcgactca
     1501 actatcagag gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca
     1561 tttgtacggc tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt
     1621 tacggtgacc gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga
     1681 aacttcggct tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt
     1741 gcacgacgac atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg
     1801 gcagcgcaat gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc
     1861 tatcttgctg acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga
     1921 actctttgat ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct
     1981 atggaactcg ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg
     2041 catttggtac agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc
     2101 aatggagcgc ctgccggccc agtatcagcc cgtcatactt gaagctaggc aggcttatct
     2161 tggacaagaa gatcgcttgg cctcgcgcgc agatcagttg gaagaatttg ttcactacgt
     2221 gaaaggcgag atcaccaagg tagtcggcaa ataatgtcta aaacaaagtt atgcacctat
     2281 taagcgcaca gcggagcaat gaaggatacc ttgatgaaaa aaattttgct gctgcatatg
     2341 ttggtgttcg tttccgccac tctcccaatc agttccgtgg cttctgatga ggttgaaacg
     2401 cttaaatgca ccatcatcgc agacgccatt accggaaata ccttatatga gaccggagaa
     2461 tgtgcccgtc gtgtgtctcc gtgctcgtct tttaaacttc cattggcaat catggggttt
     2521 gatagtggaa tcttgcagtc gccaaaatca cctacgtggg aattgaagcc ggaatacaac
     2581 ccgtctccga gagatcgcac atacaaacaa gtctatccgg cgctatggca aagcgactct
     2641 gttgtctggt tctcgcagca attaacaagc cgtctgggag ttgatcggtt cacggaatac
     2701 gtaaagaaat ttgagtacgg taatcaagat gtttccggtg actcggggaa gcataacggc
     2761 ttgacccagt catggctgat gtcgtcgctc accatatctc ccaaggagca aattcagttt
     2821 cttctacgct ttgtcgcgca taagctgcct gtatccgaag cggcttatga catggcgtat
     2881 gccacaatcc cgcagtacca ggcagccgaa ggatgggctg tacatggaaa aagcggcagc
     2941 ggctggcttc gggacaataa cggcaagata aatgaaagtc gtccgcaggg ctggttcgtg
     3001 ggctgggctg aaaaaaacgg acggcaagtt gttttcgccc gattggaaat aggaaaggaa
     3061 aagtccgata ttcccggcgg gtctaaagca cgagaggata ttctcgtgga attacccgtg
     3121 ttgatgggta acaaatgata tgtggcgtca tcgagagcag atgcataacc ctgcgctcga
     3181 gcggacctcg cgcataaagc cgcgcgagtc cgctcacctt gaacgttaga tgcactaagc
     3241 acataattgc tcacagccaa actatcaggt caagtctgct tttattattt ttaagcgtgc
     3301 ataataagcc ctacacaaat tgggagatat atcatgaaaa gagtttgatg ctcagggtgt
     3361 agcggttcgg tttattgacg acgggatcag taccgacggt gatatggggc aaatggtggt
     3421 caccatcctg tcggctgtgg cacaggctga acgccggagg atcctagagc gcacgaatga
     3481 gggccgacag gaagcaaagc tgaaaggaat caaatttggc cgcaggcgta ccgtggacag
     3541 gaacgtcgtg ctgacgcttc atcagaaggg cactggtgca acggaaattg ctcatcagct
     3601 cagtattgcc cgctccacgg tttataaaat tcttgaagac gaaagggcct cgtgatacgc
     3661 ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt
     3721 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     3781 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     3841 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     3901 tttgctcacc cagaaacgct ggtgaaagta aaa
//