From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: S.lividans lmp gene for metalloprotease.
Date: 29 Sep 92 10:10:20 GMT
Distribution: bionet
Checksum: 60534     4

LOCUS       STMLMP       1319 bp ds-DNA             BCT       29-SEP-1992
DEFINITION  S.lividans lmp gene for metalloprotease.
ACCESSION   D00670
KEYWORDS    lmp; metalloprotease.
SOURCE      S.lividans (strain 66) genomic DNA.
  ORGANISM  Streptomyces lividans
            Prokaryotae; Firmicutes; Streptomycetaceae.
REFERENCE   1  (bases 1 to 1319)
  AUTHORS   Takahashi,H.
  JOURNAL   Unpublished (1990)
  STANDARD  full staff_review
COMMENT     Submitted (31-Aug-1990) to DDBJ by:
            Hideo Takahashi
            Institute of Applied Microbiology
            University of Tokyo
            1-1-1 Yayoi, Bunkyo-ku
            Tokyo 113
            Japan
            Phone:  03-812-2111 x7825/7826
            Fax:    03-813-0539
FEATURES             Location/Qualifiers
     RBS             214..219
     CDS             226..909
                     /product="metalloprotease precursor"
                     /gene="lmp"
                     /codon_start=1
                     /translation="MRITLPLLSTAVGLGLTAAVLGTGPAATAAAPQEPVRAAQLGYQ
                     PSAGSGEDAAANRAFFEAVVKSVAEKRAANPSAAAAVTVYYSATNAPSFRSQISRSAQ
                     IWNSSVSNVRLAESSSGADFAYYEGNDSRGSYASTDGHGSGYIFLDYRQNQQYDSTRV
                     TAHETGHVLGLPDHYSGPCSELMSGGGPGPSCTNPYPNSTERSRVNQLWRYGFQAALD
                     KALEKASQR"
     sig_peptide     226..323
                     /note="signal peptide of metalloprotease"
                     /codon_start=1
     mat_peptide     324..906
                     /note="mature peptide of metalloprotease"
                     /codon_start=1
     repeat_unit     924..955
                     /note="inverted repeat"
BASE COUNT      203 a    492 c    438 g    186 t
ORIGIN
        1 gagctccatg cagggagcgt aaagcgaaca gttggttgcg ccaggtgaac aaaacgcggc
       61 ggatcagggc gagttctgca ctctggtcaa agctggaacg agagtggccg ggcggtgggt
      121 gatagcccgg ccctatcacc tcttgccatc atcacagcgg gctcatgggc gccccacact
      181 caccggtgac gacttctccc cactccccca ctcaaggagt catcgatgcg tatcaccctg
      241 ccccttcttt ccaccgcggt cggtctcggc ctgacggccg ccgtgctcgg caccggcccc
      301 gccgcgacgg ccgcggcgcc ccaggagccg gtcagagccg cccagctcgg ctaccagccc
      361 tcggccggct cgggcgagga cgcggccgcc aaccgcgcgt tcttcgaggc ggtcgtcaag
      421 tccgtcgccg agaagcgcgc cgccaacccg tccgccgccg cggccgtcac cgtctactac
      481 agcgccacca acgcgccgag cttccgttcc cagatatccc gctccgccca gatctggaac
      541 agctcggtgt ccaacgtacg gctcgcggag tcgagttccg gcgcggactt cgcgtactac
      601 gagggcaacg actcgcgcgg ctcgtacgcg tccacggacg ggcacggcag cggctacatc
      661 ttcctcgact accgccagaa ccagcagtac gactcgaccc gcgtgaccgc ccacgagacc
      721 gggcacgtgc tcggcctgcc cgaccactac tccgggccgt gcagcgagct gatgtcgggc
      781 ggcggccccg gcccgtcctg caccaacccc tacccgaact ccaccgagcg cagccgggtg
      841 aaccagctgt ggcgctacgg cttccaggcc gccctcgaca aggcgctgga gaaggcctcc
      901 cagcgctgac gtacgcggac caccgtgcgg gcggcccggc cgggccgccc gcacgcgtgc
      961 gcgctccctc cacctccgct tgggcaacct tcctgcgggt actcgcagtt cactggagag
     1021 cggcatcccg aaggagtcgg acgtggacga acggaaggca cctgtcgacg acggtctcgg
     1081 cgacctggag ccgggcacga ggcagcgcac gcagttgcgc gttcgcgtcg cagacatcgc
     1141 ctggacggcg gcgtgcgcgg tgctcgcgct gtgggccgtg tggaccgcga tcgatctcgc
     1201 gcggggcgcg acggcgtggg cgtactgcgc cgtggcctgg ctgctgctcg cggtggcact
     1261 gaccgtgcgc gtgatggcgg tccggcgcag gacgtcgctg tagcggcaag accccggac
//