From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Saccharomyces cerevisiae O-6-methylguanine-DNA methyltransferase
Date: 31 Jul 92 10:02:09 GMT
Distribution: bionet
Checksum: 45903     4

LOCUS       YSCMGT1WEI   1664 bp ds-DNA             PLN       31-JUL-1992
DEFINITION  Saccharomyces cerevisiae O-6-methylguanine-DNA methyltransferase
            (MGT1) gene, complete cds.
ACCESSION   M94227
KEYWORDS    6-O-methylguanine-DNA methyltransferase.
SOURCE      Saccharomyces cerevisiae (strain DBY747) DNA.
  ORGANISM  Saccharomyces cerevisiae
            Eukaryota; Fungi; Eumycota; Hemiascomycetes; Endomycetales;
            Saccharomycetaceae.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Xiao,W. and Samson,L.
  TITLE     The Saccharomyces cerevisiae MGT1 DNA repair methyltransferase
            gene: Its promoter and entire coding sequence, regulation and in
            vivo biological functions
  JOURNAL   Nucleic Acids Res. 20, 3599-3606 (1992)
  STANDARD  full staff_entry
FEATURES             Location/Qualifiers
     protein_bind    510..519
                     /bound_moiety="repressor"
                     /standard_name="upstream repression site (URS)"
                     /gene="MGT1"
     TATA_signal     586..592
                     /gene="MGT1"
     TATA_signal     620..627
                     /gene="MGT1"
     TATA_signal     669..676
                     /gene="MGT1"
     CDS             720..1340
                     /product="O-6-methylguanine-DNA methyltransferase"
                     /gene="MGT1"
                     /codon_start=1
                     /translation="MHKKKIENGRIFDLNGPTMKELLYYTFIETEVTGAFLVFREKTQ
                     NLVFASLGNDKLFLLGKVEGFLKKHEKQDTMYDLQELKEAETYKKSIENYTICLENKM
                     PLPSGAIPFEFLFGTDFQRKVWNELLNVEHGHVVTYGDIAKRIGKPTAARSVGRACGS
                     NNLALLVPCHRIVGSNRKLTGYKWSCKLKEQLLNNEKENSLSLSRL"
     mat_peptide     774..1337
                     /product="O-6-methylguanine-DNA methyltransferase"
                     /gene="MGT1"
                     /codon_start=1
BASE COUNT      533 a    304 c    310 g    517 t
ORIGIN
        1 actagtctca aacgttttat tggattttta agtggttcgt cagcttaata acccctatac
       61 ttattgaccg tctgtcttgg aagttttatc ttattccatc tttatcttcc tttatttcca
      121 ttatatttgt gctgaagatt tttcctatag aaacccgtga cgaaaggtta gattctgatg
      181 atgattctac cggaaacggt agcggtaacc atgatgatgt ttttgacgat accggttctg
      241 aattctcctc ctcaccatct ttttccccgt atcagataaa cactctagga agtagtatca
      301 aacaaaataa tcaagcctat tcatctattc aaaatgagca gattctaccc aaaaacggaa
      361 acttatcgaa ccagacacac ggttctgcac aaaatgttta tttcattaca tctgattcag
      421 gaccatcaag gactggagaa tttttcagtt ttcataacag aaccgatcca aatatcagcg
      481 acaacattgc tgcgaataaa cccagttcag gtggaggcca gaattcgcct ggagacatgg
      541 cagttgccta gtatggcctc ataaaaatat ttgcttcatc ctaagtataa atgtttagtt
      601 ttatcctaca tacatattct tattatagtc attagaaaaa aggcacggcg ctattggcct
      661 ggcagggcat ttaaaatgcg gtggaaacaa ggaagattaa tcaagtaatg atatagcata
      721 tgcacaaaaa aaaaattgaa aacggtcgca tttttgatct aaatggacca acgatgaagg
      781 aactgcttta ctatacattc attgaaactg aagtgactgg tgcatttttg gtgtttaggg
      841 aaaagactca aaaccttgtt tttgcctcgt taggtaatga taagcttttt ttattgggaa
      901 aggtggaagg cttcttgaag aaacatgaga aacaggatac aatgtacgat ttacaggaac
      961 taaaagaggc agaaacatat aagaaatcaa tcgaaaatta tacaatatgt ttagaaaaca
     1021 aaatgccatt accatcgggc gctattccct ttgagttcct gtttggaaca gattttcaac
     1081 gtaaagtttg gaatgagctt ttaaacgtgg aacacggcca cgtcgtaaca tatggtgata
     1141 ttgcaaagag aatagggaag ccaactgccg caagatctgt cggaagagct tgcggctcaa
     1201 ataacctggc attgttagta ccttgccata gaatcgttgg tagcaataga aaattaaccg
     1261 gatataaatg gagctgtaaa ctgaaagaac agttattaaa taatgaaaag gaaaatagct
     1321 taagccttag tagattgtag ttggataaag gattttagga aaataaacat aagaaatagt
     1381 tatgtatatg tggtaaattg ttctagttat acatctatgt tacaatatgg ctggcgttgg
     1441 taatcttgtg aaacaagcga cgaacttgtc acccttcttt ctttccactt ttttaccttc
     1501 ttctgtggca tttctcatta ttttcacaca ttcatcgttt tcttcccatt ctttgctcaa
     1561 ccgttcaaat aaaggatgct cttctaaatg ttttaccatc cactcatgca ggtctttgac
     1621 gtctgtaatt gtgtacacga caccgccctc tttcaaaaca tatg
//