From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Equine infectious anemia virus envelope protein (env); reverse
Date: 1 Sep 92 10:01:06 GMT
Distribution: bionet
Checksum: 38712     7

LOCUS       EIATTTAENR   1059 bp ss-mRNA            VRL       01-SEP-1992
DEFINITION  Equine infectious anemia virus envelope protein (env); reverse
            transcriptase (rev); trans-activator protein (tat); unknown protein
            (ttm) mRNA, exons 1-4.
ACCESSION   M93674
KEYWORDS    envelope protein; reverse transcriptase; trans-activator protein.
SOURCE      Equine infectious anemia virus (strain Wyoming pathogenic) cDNA to
            mRNA.
  ORGANISM  Equine infectious anemia virus
            Viridae; ss-RNA enveloped viruses; Positive strand RNA virus;
            Retroviridae; Lentivirinae.
REFERENCE   1  (bases 1 to 1059)
  AUTHORS   Stephens,R.M., Derse,D. and Rice,N.R.
  TITLE     Cloning and characterization of cDNAs encoding the equine
            infectious anemia virus tat and putative rev proteins
  JOURNAL   J. Virol. 64, 3716-3725 (1990)
  STANDARD  full staff_entry
REFERENCE   2  (bases 1 to 1059)
  AUTHORS   Beisel,C.E., Edwards,J.F., Dunn,L.L. and Rice,N.R.
  TITLE     Analysis of multiple mRNAs from pathogenic equine infectious anemia
            virus (EIAV) in an acutely infected horse reveals a novel protein,
            ttm, derived from the carboxy terminus of the EIAV transmembrane
  JOURNAL   Unpublished (1992)
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     exon            <1..164
                     /number=1
                     /evidence=EXPERIMENTAL
                     /citation=[2]
     CDS             join(78..164,408..>1059)
                     /function="unknown"
                     /note="ttm is encoded by the 2-exon transcript, and is
                     derived from the C-terminus of the EIAV transmembrane
                     protein. Non-AUG translation initiation at CTG. Full CDS
                     has additional 2bp before stop codon."
                     /gene="ttm"
                     /evidence=EXPERIMENTAL
                     /codon_start=1
                     /translation="MADRRIPGTAEENLQKSSGGVPGQNTGGQILRALWKVTSGAGSS
                     GSRYLKKKFXRKHASREDTWDQAQHNIHLAGVTGGSGNKYYRQKYSRNDWNGESEEYN
                     RWPKSWVKSIEAFGESYISEKTKGEISQPGATINKHKNGAGGNNPHQGSLDLEIRSEG
                     GNIYDCCIKAQEGTLAIPCCGFPLWLFWGLLIILGRIAGYGLRGLAVIIRICIRGLNL
                     IFEIIRKMLDYIGRALNPGTSHVSMPQY"
                     /citation=[2]
                     /partial
     mRNA            join(<1..164,408..>1059)
                     /note="two exon transcript"
                     /gene="ttm"
                     /evidence=EXPERIMENTAL
                     /citation=[2]
                     /partial
     mRNA            join(<1..306,408..>1059)
                     /note="three exon transcript"
                     /gene="tat"
                     /evidence=EXPERIMENTAL
                     /citation=[2]
                     /partial
     mRNA            <1..>1059
                     /note="four exon transcript"
                     /evidence=EXPERIMENTAL
                     /citation=[2]
     CDS             78..305
                     /transl_except=(pos:78..80,aa:OTHER)
                     /note="Tat is encoded by both the 4-exon and 3-exon
                     transcripts. Non-AUG translation initiation at CTG."
                     /product="trans-activating factor"
                     /gene="tat"
                     /evidence=EXPERIMENTAL
                     /codon_start=1
                     /translation="XADRRIPGTAEENLQKSSGGVPGQNTGGQEAGPNYHCQLCFLRS
                     LGIDYLDPSLRKKNKQRLKAIQQGRQPQYLL"
                     /citation=[1]
     exon            165..306
                     /number=2
                     /evidence=EXPERIMENTAL
                     /citation=[2]
     exon            307..407
                     /number=3
                     /evidence=EXPERIMENTAL
                     /citation=[2]
     CDS             317..814
                     /number=3
                     /note="rev is encoded by the 4-exon transcript"
                     /gene="rev"
                     /evidence=EXPERIMENTAL
                     /codon_start=1
                     /translation="MAESKEARDQEMNLKEESKEEKRRNDWWKIDPQGPLESDQWCRV
                     LRQSLPEEKIXSQTCIARRHLGPGPTQHTPSRRDRWIREQILQAEVLQERLEWRIRGV
                     QQVAKELGEVNRGIWRELHFREDQRGDFSAWGDYQQAQERRWGEQSSPRVLRPGDSKR
                     RRKHL"
                     /citation=[1]
     exon            408..>1059
                     /number=4
                     /evidence=EXPERIMENTAL
                     /citation=[2]
     variation       replace(479..480,"ct")
                     /frequency=0.2
                     /note="in clones of PCR-amplified cDNA"
                     /citation=[1]
                     /citation=[2]
     variation       replace(479..480,"tc")
                     /frequency=0.8
                     /note="in clones of PCR-amplified cDNA"
                     /citation=[1]
     unsure          replace(479..480
                     /note="primary sequence is from uncloned PCR-amplified
                     cDNA, gels show equal intensity C and T at 479,
                     predominantly T at 480"
                     /citation=[2]
BASE COUNT      337 a    213 c    276 g    220 t     13 others
ORIGIN
        1 ctagtttgtc tgttcgagat cctacagttg nnnyyygwac wgggacctga gangggcgca
       61 gnccctacct gttgaacctg gctgatcgta ggatccccgg gacagcagag gagaacttac
      121 agaagtcttc tggaggtgtt cctggccaga acacaggagg acaggaagcg ggacccaact
      181 accattgtca gctgtgtttc ctgaggtctc taggaattga ttacctcgat ccttcattaa
      241 ggaagaagaa taaacaaaga ctgaaggcga tccaacaagg aagacaacct caatatctgt
      301 tataagtaag aacagcatgg cagaatcgaa ggaagcaaga gaccaagaaa tgaacctgaa
      361 agaagaatct aaagaagaaa aaagaagaaa tgactggtgg aaaatagatc ctcagggccc
      421 tctggaaagt gaccagtggt gcagggtcct ccggcagtcg ctacctgaag aaaaaattyy
      481 atcgcaaaca tgcatcgcga gaagacacct gggaccaggc ccaacacaac atacacctag
      541 caggcgtgac cggtggatca gggaacaaat actacaggca gaagtactcc aggaacgact
      601 ggaatggaga atcagaggag tacaacaggt ggccaaagag ctgggtgaag tcaatcgagg
      661 catttggaga gagctacatt tccgagaaga ccaaagggga gatttctcag cctggggcga
      721 ctatcaacaa gcacaagaac ggcgctgggg ggaacaatcc tcaccaaggg tccttagacc
      781 tggagattcg aagcgaagga ggaaacattt atgactgttg cattaaagcc caagaaggaa
      841 ctctcgctat cccttgctgt ggatttccct tatggctatt ttggggactg ttaattatac
      901 taggacgcat agcaggctat ggattgcgtg gactcgctgt tataataagg atttgtatta
      961 gaggcttaaa tttgatattt gaaataatca gaaaaatgct tgattatatt ggaagagctt
     1021 taaatcctgg cacatcycat gtatcaatgc ctcagtatg
//