From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: TOL Plasmid pWW53 (Pseudomonas putida strain MT53) resolvase and
Date: 21 Jan 93 05:50:25 GMT
Distribution: bionet
Checksum: 22478 4

LOCUS       W53XYL       1447 bp ds-DNA             BCT       20-JAN-1993
DEFINITION  TOL Plasmid pWW53 (Pseudomonas putida strain MT53) resolvase and
            xylS3 positive regulator genes, complete coding regions.
ACCESSION   L02643
KEYWORDS    positive regulator; resolvase; xylS3 gene.
SOURCE      Plasmid pWW53 DNA.
  ORGANISM  Plasmid pWW53
            Prokaryotae.
REFERENCE   1  (bases 1 to 1447)
  AUTHORS   Assinder,S.J., De Marco,P., Sayers,J.R., Shaw,L.E., Winson,M.K. and
            Williams,P.A.
  TITLE     Identical resolvases are encoded by Pseudomonas TOL plasmids pWW53
            and pDK1
  JOURNAL   Nucleic Acids Res. 20, 5476-5476 (1992)
  STANDARD  full automatic
REFERENCE   2  (bases 1 to 1447)
  AUTHORS   Assinder,S.J., De Marco,P., Sayers,J.R., Shaw,L.E., Winson,M.K. and
            Williams,P.A.
  TITLE     Identical resolvases are encoded by Pseudomonas TOL plasmids pWW53
            and pDK1
  JOURNAL   Nucleic Acids Res. 20, 5476-5476 (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     CDS             34..579
                     /citation=[2]
                     /note="based on amino acid sequence homology with known
                     resolvases; putative"
                     /product="resolvase"
                     /codon_start=1
                     /translation="MRLFGYARVSTSQQSLDVQIKALKAENVRATRIFTDKVSGSHVN
                     REGLRMLRLKVEEGDVVLVKKLDRLGRDTADMIQLIKEFDDMGVAIRFLDDGISTEGT
                     MGKMVVTILSAVAQAERLRILERTNEGRLEAKAKGVKFGRKPTVDKAEVFTLHGQGIS
                     AMEIAKRLKIGRSTVYKVLAS"
     CDS             952..1425
                     /gene="pWW53xylS3"
                     /citation=[1]
                     /note="identified by homology with C-terminal of plasmid
                     pWW0 xylS protein; putative"
                     /product="positive regulator"
                     /codon_start=1
                     /translation="MSMTLVAEHYTKIIATKLLETLSSNVSRKGFVEGNPCFERVVQF
                     IEENVKRSISLEQLAELALMSPRSLYTMFEKHTGTTPMNYIRNRKLECVRACLSNPTT
                     NIRSITEVALDYGFLHLGRFAEKYRSTFGELPSDTLSLHKMKCIDSRESSLSSLF"
BASE COUNT      358 a    379 c    388 g    322 t
ORIGIN      
        1 agatctaaaa atcgacttat gagacatttt gccatgcgac tttttggtta tgcccgcgtc
       61 tccaccagtc aacagtccct tgatgtacag ataaaggcgc tcaaggctga aaacgtcaga
      121 gcaacccgaa tttttaccga caaggtatct ggcagtcacg tgaaccgcga aggcctaagg
      181 atgctccggc tcaaggtcga agaaggtgat gtggtcctgg taaaaaaact cgaccggctc
      241 ggtcgcgata ccgcggacat gatccagctc attaaggagt tcgacgacat gggcgtggcc
      301 atccgatttt tggatgacgg catcagcaca gagggaacca tgggcaaaat ggtggtgacc
      361 atcctgtcag ccgtggctca ggccgaacgc ctgcggatac tggaacggac caacgaaggt
      421 cgtttggaag caaaggccaa gggcgtgaag tttggccgca aacctacggt ggacaaagcc
      481 gaggtattca cgctccacgg tcagggtatc agtgcgatgg aaattgccaa acgactgaag
      541 attggtcgct cgacggtcta caaagtgctg gcatcataag ttcatctgac agacgcgtgc
      601 tgtcagcccc gcccatgctt agcgtacttt aaaatccctt ttctgaggcg cccccacctg
      661 cagctattgc tcaagggtca ttgcctttgg cgtggcaatg gcttggagca ctattttgcg
      721 ccaggcgaac tattggtgat caacccggat gaccgcgcgg agctgactta ttcggaggag
      781 tgcgagaaat tcattgtcaa attgccctcg gttatcattg atagcgcatg caatgagctc
      841 aattggcaga agccgagtgg aggtatccgt ttcccccccc gacatagcct gcagcagctt
      901 aatggcttca tcaatctaat cggactggtt tgtgacgaag cggaacataa catgtcgatg
      961 accttggtgg cggagcacta cacgaagatc atcgctacca agctgctcga gacgctcagc
     1021 agcaatgtca gccgcaaagg cttcgtcgaa ggcaacccat gtttcgagcg agtcgttcag
     1081 ttcattgagg aaaatgtcaa acggagtatc agccttgagc agttagcgga gctggcgctg
     1141 atgagcccac gctccctcta caccatgttc gagaagcata ccggcaccac gcccatgaat
     1201 tacattcgca accgcaagct tgagtgcgtc cgtgcctgct tgagcaatcc cactaccaat
     1261 atacgtagta tcactgaggt ggccctagac tacggcttct tacatctggg acgtttcgcc
     1321 gagaaataca ggagcacatt cggcgagctg ccttccgaca ccctgagtct acacaagatg
     1381 aagtgcatcg attcaaggga gtctagcctg tcctcgctct tttgactggc tgcttgtcgg
     1441 acttgag
//