From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Bovine vacuolar H+-ATPase mRNA, complete cds.
Date: 9 Apr 92 10:02:22 GMT
Distribution: bionet
Checksum: 05201     6

LOCUS       BOVATPX      2681 bp ss-mRNA            MAM       09-APR-1992
DEFINITION  Bovine vacuolar H+-ATPase mRNA, complete cds.
ACCESSION   M88690
KEYWORDS    active proton transport; vacuolar H+-ATPase.
SOURCE      Bos taurus (library: lambda gt11) adult brain cDNA to mRNA.
  ORGANISM  Bos taurus
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Artiodactyla; Ruminantia; Pecora; Bovidae; Bovinae;
            Bovini.
REFERENCE   1  (bases 1 to 2681)
  AUTHORS   Nelson,R.D., Guo,X.-L., Masood,K., Brown,D., Kalkbrenner,M. and
            Gluck,S.
  TITLE     Selectively amplified expression of an isoform of the vacuolar
            H+-ATPase 56-kilodalton subunit in renl intercalated cells
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 89, 3541-3545 (1992)
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     5'UTR           1..23
     CDS             24..1553
                     /function="active proton transport"
                     /standard_name="H+ ATPase"
                     /note="brain isoform, 56 kilodalton subunit"
                     /product="vacuolar H+-ATPase"
                     /codon_start=1
                     /translation="MALRAMRGIVNGAAPELPVPTSGLAGSREQALAVSRNYLSQPRL
                     TYKTVSGVNGPLVILDHVKFPRYAEIVHLTLPDGTKRSGQVLEVSGSKAVVQVFEGTS
                     GIDAKKTSCEFTGDILRTPVSEDMLGRVFNGSGKPIDRGPVVLAEDFLDIMGQPINPH
                     FRIYPEEMIQTGISAIDGMNSIARGQKIPIFSAAGLPHNEIAAQICRQAGLVKKSKDV
                     VDYSEENFAIVFAAMGVNMETARFFKSDFEENGSMDNVCLFLNLANDPTIERIITPRL
                     ALTTAEFLAYQCEKHVLVILTDMSSYAEALREVSAAREEVPGRRGFPGYMYTDLATIY
                     ERAGRVEGRNGSITQIPILTMPNDDITHPIPDLTGYITEGQIYVDRQLHNRQIYPPIN
                     VLPSLSRLMKSAIGEGMTRKDHADVSNQLYACYAIGKDVQAMKAVVGEEALTSDDLLY
                     LEFLQKFEKNFITQGPYENRTVYETLDIGWQLLRIFPKEMLKRIPQSTLSEFYPRDSA
                     KH"
                     /partial
     3'UTR           1554..2681
BASE COUNT      616 a    638 c    681 g    746 t
ORIGIN
        1 gaattcggcc gcgaggagac aagatggcgc tgcgggcgat gcgggggatc gtgaacgggg
       61 ccgcgcctga gctaccagta cccaccagcg ggctggcggg gtctcgagag caggcgctgg
      121 cagtgagccg aaactacctc tcccagcctc gtctcaccta caagactgtc tctggagtga
      181 atggtccact agtgatctta gatcatgtaa agtttcccag atatgctgag attgtccact
      241 tgacattacc agatggcaca aaaagaagtg ggcaagttct agaagttagt ggctccaaag
      301 ctgtggttca ggtatttgaa ggaacatccg gcatagatgc caagaaaaca tcctgtgagt
      361 ttactggaga tattctccgc acaccagtgt ctgaggatat gcttggtcga gtattcaatg
      421 gatcaggaaa acccattgac cgaggtcctg tggtgttggc cgaagacttc cttgacatca
      481 tgggtcagcc aatcaaccct cattttcgca tctacccaga agagatgatt cagactggca
      541 tttctgccat cgacggcatg aacagtattg cgaggggaca gaaaatcccc atcttttctg
      601 ctgccgggtt accacacaac gagattgcag ctcagatctg tcgccaggct ggtttggtaa
      661 agaaatccaa agacgtggta gactacagtg aagaaaactt tgccattgtg tttgctgcta
      721 tgggagtaaa catggaaaca gcccggttct tcaaatctga ctttgaagaa aatggctcaa
      781 tggacaatgt ctgccttttc ttgaatctgg ctaatgaccc aactatcgag aggatcatca
      841 ctcctcgcct ggctctgacc accgctgagt ttctggctta ccagtgtgag aagcatgtcc
      901 tggtcatcct gacagatatg agttcttacg ctgaagcact tcgagaggtt tcagctgcca
      961 gggaagaggt tcctggtcgg cgaggcttcc ccggctacat gtatacggat ttagccacca
     1021 tctatgaacg cgctgggcga gtggaaggta gaaatggctc tattacccaa atccctattc
     1081 tcaccatgcc caatgatgat atcactcatc ctatccctga cttgactggg tatattactg
     1141 agggccagat ctatgtggac agacagctgc acaacagaca gatttaccct cctattaatg
     1201 tgctgccctc actctctcgg ttaatgaagt cagctattgg agaaggaatg accaggaagg
     1261 atcatgctga tgtgtctaac cagttgtacg catgctatgc tatcggtaag gatgtgcaag
     1321 ccatgaaagc tgtggtggga gaagaagccc tgacctcaga tgacctcctt tacttggaat
     1381 ttctgcagaa gtttgagaaa aacttcatta ctcagggtcc ctatgaaaat cgaactgtct
     1441 atgagacttt ggacattggc tggcagttgc ttcgaatctt ccccaaagaa atgctgaaga
     1501 ggatccctca gagtaccctg agcgaatttt accctcgaga ctctgcaaag cactagctgc
     1561 tgctgcttgt gcggctcgac cctcttgtca agtgctggtt ctgtttgctg attccttttg
     1621 cactcctcca tccacctgtg tgtgggagtt cacctgttac cctgtaatta aagacaaagg
     1681 ctaggtaact gttgtgccag tgttcagcgt ttaaactgct aaccgattga gagatccccg
     1741 ctcagaacct caccttctgt gctgtcttta aagtggcgga ggtgaggctt gcttaccggt
     1801 gtatctattt gtacatagtg gagagctagt tgcgaataat gtcttgtttg ggtctcccaa
     1861 accctacctc tcaactccct taagagtatc aactgttttg aagttaaaat gcttcagtct
     1921 caaatttagg ggcaaggtgg agactggaag aattctcctt tcagaagaac catgaggctc
     1981 gtggctgagc tccctctgga gtactagtgt acctgtgggt ctgtcctctg ctctgtgcag
     2041 atgggtttta ctgtctgctt gagttttctt aggaaaagag ttctgttctg ccagtgctgc
     2101 gagttgggat tcctgtgtgg ccatctttct ctttgaggcc taaagagtca gcaccactgt
     2161 gcagcggcat tctcctgcag gggtggcgtg ccttgtgctg atgaccccac tgggctgcag
     2221 tcataggaga actgagactt ggaaaatgct ggggcacagt taagaaaacc tacatcccac
     2281 cctcatcttg tgtttatggt ggcttaggtc tctgcattgc cctccagatc ctgaggtggg
     2341 gcatggagat gacttgcctt aggtttgtgg atgctttaaa ctctgctcag tcctcaagct
     2401 ttctgactca gctctccctt ttctggttga tcttgtggca cgtgtagcaa tgtttctttc
     2461 attcctgccc cttcctggct tgagctctta gctgtattct gtgtgcctct gccgtgtctg
     2521 ctgtttgggt ctctgtgctg tgtgttctca ggtgcagcca taacttcccc actccgagca
     2581 ttccaccttc cagttgtttt tctctgaggg gatggggggg cggtcagcat gattatattt
     2641 taatgtagaa aatgtgacat ctcgttataa atgcggaatt c
//