From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Escherichia coli DNA binding protein (phoM) gene, complete cds.
Date: 28 Jul 92 10:02:47 GMT
Distribution: bionet
Checksum: 54272     5

LOCUS       ECOPHOMROB   1946 bp ds-DNA             BCT       28-JUL-1992
DEFINITION  Escherichia coli DNA binding protein (phoM) gene, complete cds.
ACCESSION   M97495
KEYWORDS    phoM operon.
SOURCE      Escherichia coli (sub_strain W3110, strain K-12) (library: EMBL4)
            DNA.
  ORGANISM  Escherichia coli
            Prokaryotae; Gram-negative facultatively anaerobic rods;
            Enterobacteriaceae.
REFERENCE   1  (bases 1 to 1946)
  AUTHORS   Amemura,M., Makino,K., Shinagawa,H. and Nakata,A.
  TITLE     Nucleotide sequence of the phoM region of Escherichia coli: four
            open reading frames may constitute an operon
  JOURNAL   J. Bacteriol. 168, 294-302 (1986)
  STANDARD  full staff_review
REFERENCE   2  (bases 1 to 1946)
  AUTHORS   Skarstad,K., Thony,B., Hwang,D. and Kornberg,A.
  TITLE     A novel binding protein of the origin of the E. coli chromosome
  JOURNAL   J. Biol. Chem. (1992) In press
  STANDARD  simple staff_entry
FEATURES             Location/Qualifiers
     CDS             1..87
                     /note="ORF1 in phoM operon"
                     /product="DNA binding protein"
                     /gene="phoM"
                     /codon_start=3
                     /translation="RPNQSLLHEPMAPALSGLKTGSNACISY"
                     /citation=[1]
                     /partial
     RBS             282..288
                     /standard_name="Shine-Dalgarno"
     CDS             295..1164
                     /product="DNA binding protein"
                     /gene="phoM"
                     /evidence=EXPERIMENTAL
                     /codon_start=1
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
BASE COUNT      466 a    523 c    470 g    487 t
ORIGIN
        1 gtcgaccgaa ccaatctctt ctgcatgagc caatggcccc agcattatca ggcttaaaga
       61 caggatcaaa tgcttgtatt tcatattgtt accattttta tctattacac gagcaattag
      121 ttcgtcacgg taaataacat attgattaag aacaaaatct caatactttt atttccgtca
      181 agccctaaaa catactctac taagaaaaaa acactgaatg ctaaaacagc aaaaaatgct
      241 attatccaat tacctgatgt caggtgctcg ttgttgaaag gatgaggata ttttatggat
      301 caggccggca ttattcgcga ccttttaatc tggctggaag gtcatctgga tcagcccctg
      361 tcgctcgaca atgtagcggc gaaagcaggt tattccaagt ggcacttaca gagaatgttt
      421 aaagatgtca ctggccatgc tattggcgcg tatattcgtg ctcgtcgttt gtcgaaatcg
      481 gcggtcgcac tacgcctgac tgcgcgtccg attctggaca tcgcgctgca ataccgcttc
      541 gactctcaac agacatttac ccgcgcattc aagaagcagt ttgcccagac tcctgcactt
      601 taccgccgtt ctcctgaatg gagcgccttt ggtattcgcc cgccgctacg cctgggtgaa
      661 ttcactatgc cagagcacaa atttgtcacc ctggaagata cgccgctgat tggtgttacc
      721 cagagctact cctgttcgct ggagcaaatc tctgatttcc gccatgaaat gcgttatcag
      781 ttctggcacg attttctcgg caacgcgccg accattccgc cggtgctcta cggcctgaat
      841 gaaacgcgtc cgagtcagga taaagacgac gagcaagagg tattctatac caccgcgtta
      901 gcccaggatc aggcagatgg ctatgtactg acggggcatc cggtgatgct gcagggcggc
      961 gaatatgtga tgtttaccta tgaaggtctg ggaaccggcg tgcaggagtt tatcctgacg
     1021 gtatacggaa cgtgcatgcc aatgctcaac ctgacgcgcc gtaaaggtca ggatattgag
     1081 cgatactacc cggcagaaga tgccaaagcg ggagatcgcc caattaatct acgctgtgaa
     1141 ctgctgattc cgatccgtcg ttaacgctgc agctcatcta atgcaggggc gtccagatgc
     1201 gagatgtccc ctgcggtttc aacgacccag ccagacgcca gccacaggct ttcctgataa
     1261 tccacgcgcg aaatagaaca gttacgcaga cgtaagcggc gctctgccca tgctggtaat
     1321 ccgagaatcg tactcaccag gcatcccagt gcaataccat gactcaggag cagcggtcgg
     1381 cttccctgcg gtaagtcccg gcaagattcc agtgccgcat tgacgcgatc gctgagctcc
     1441 tgcattgact cgccttcagg aatacgcccg tcaacggtgc cattgaccag ctgccgacgc
     1501 cagttctctt cttcttcggt cagagaatcg atatgtcttt tttccagcac acccatgttt
     1561 aattcacgca ggcgagaatc aaagatgatg tcacagccgc aggcctgggc gatgatttcc
     1621 gccgtacgcc gggtgcgtcc taaatcgcta ctgatgatat gcgtaatgcc aagctctttg
     1681 gcacgggttg ccacctgcat cgcctgttgc tcacctttgg cggtcagcgg gctgtcagac
     1741 tggccctgaa tacgtcgctc ggcgttccac tgcgtttcac cgtggcggac taggtatacc
     1801 tgtaacatgc tttttttccg tatactgctg taacgttaat tttctgagta atgctgatta
     1861 tgcaccaagt tgtctgtgcg accaccaatc ccgctaaaat tcaggccatt ctgcaggcat
     1921 ttcacgagat cttcggcgaa ggatcc
//