From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: M.capricolum rpmH, dnaA and dnaN genes.
Date: 10 Sep 92 10:34:11 GMT
Distribution: bionet
Checksum: 19545     7

LOCUS       MYCDNAA      2517 bp ds-DNA             BCT       10-SEP-1992
DEFINITION  M.capricolum rpmH, dnaA and dnaN genes.
ACCESSION   D90426
KEYWORDS    DNA polymerase III; DnaA protein; DnaN protein; dnaA gene;
            dnaN gene; ribosomal protein H; rpmH gene.
SOURCE      M.capricolum (strain ATCC27343) DNA.
  ORGANISM  Mycoplasma capricolum
            Prokaryotae; Tenericutes; Mollicutes; Mycoplasmas; Mycoplasmatales;
            Mycoplasmataceae.
REFERENCE   1  (bases 1 to 2517)
  AUTHORS   Fujita,M.Q., Yoshikawa,F. and Ogasawara,N.
  TITLE     Structure of the dnaA and DnaA-box region in the Mycoplasma
            capricolum chromosome: conservation and variation in course of
            evolution
  JOURNAL   Gene 110, 17-23 (1992)
  STANDARD  full staff_review
COMMENT     Data kindly submitted in computer readable form by:
            Masaki Q. Fujita
            Department of Pathology and Genetics
            Osaka University, Medical School
            2-2 Yamadaoka
            Suita, Osaka 565
            Japan
            Phone:  06-877-5111 X 5426
            Fax:    06-878-6336
FEATURES             Location/Qualifiers
     CDS             complement(<1..75)
                     /note="ribosomal protein H(aa 1-25)"
                     /codon_start=1
                     /translation="MKRTWQPSKLKHARVHGFRARMATK"
     RBS             complement(81..91)
                     /note="SD sequence for rpmH"
     -10_signal      complement(147..152)
                     /note="-10 sequence for rpmH"
     -35_signal      complement(169..174)
                     /note="-35 sequence for rpmH"
     RBS             361..368
                     /note="SD sequence for dnaA"
     CDS             371..1723
                     /note="DnaA protein"
                     /codon_start=1
                     /translation="MNLNDILKELKLSLIANKNIDESVYNDYIKTINIYKKDLSNYVV
                     VVKSQFGLLAIKQFRPTIENEIKKILKQPVNISFTYEQEYQKQLEKTESINKDHSDII
                     SKKNKKVNENTFENFVIGSSNEQAFIAVQTVSKNPGISYNPLFIYGESGMGKTHLLKA
                     AKNYIESNFSDLKVSYMSGDEFARKAVDILQKTHKEIEQFKNEVCQNDVLIIDDVQFL
                     SYKEKTNEIFFTIFNNFIENDKQLFFSSDKSPELLNGFDNRLITRFNMGLSIAIQKLD
                     NKTATAIIKKEIKNQNIKTEVTNEAINFISNYYSDDVRKIKGSVSRLNFWSQQNPEEK
                     VITIEIISDLFRDIPTSKLGILNVKKIKEVVSEKYGISVNAIDGKARSKSIVTARHIA
                     MYLTKEILNHTLAQIGEEFGGRDHTTVINAERKIEMMLKKDKQLKKTVDILKNKILTK
                     "
     RBS             1876..1882
                     /note="SD sequence for dnaN"
     CDS             1888..>2517
                     /note="DnaN protein (aa 1-210)"
                     /codon_start=1
                     /translation="MNFSINRIVLLDNLSKAAKVIDYKNVNPSLSGIYLNVLNDQVNV
                     ITTSGILSFKSILNNQNSDLEVKQEGKVLLKPKYVLEMLRRLDDEFVVFSMVEDNELI
                     IKTNNSDFSIGVLNSEDYPLIGFREKGIEFNLNPKEVKKTIYQVFVSMNENNKKLILT
                     GLNLKLNNNQAIFSTTDSFRISQKILEIQSDNNEDIDITIPFKTALELPK"
BASE COUNT     1085 a    242 c    308 g    882 t
ORIGIN
        1 tttagtagcc attcttgctc taaaaccatg aacacgtgca tgttttaatt ttgatggttg
       61 tcaagttctt ttcatatcac ttacctcctt tacaaaaaaa taaataattc acacctaatt
      121 atataatatt ttgtattaaa aatacactta taaacttatt ttttagctta tcaattaaat
      181 gtagataaaa ctaaaaatta ctcacaaaaa tattagttaa ttgttgataa gttgataaat
      241 aataaattca aggaactaat taagatttta tatttttgca ttttgttaat ataactataa
      301 tgtatggatt ttctccacgt tttccacatt tttaacaagt ctttacttta taatattttt
      361 ggagataaat atgaacctaa acgatatttt aaaagaactc aaactaagtt taatagcaaa
      421 taaaaatatt gatgaatctg tgtataacga ctatattaaa acaattaata tttataaaaa
      481 agatttatct aattatgttg ttgttgttaa atcacaattt ggcttattgg ctataaaaca
      541 atttcgtcca actattgaaa atgagataaa aaagatttta aaacaaccag taaatattag
      601 ttttacatat gaacaagaat atcaaaaaca attagaaaaa actgaatcaa taaataaaga
      661 tcattccgat attattagta aaaaaaataa aaaagtaaat gaaaatacat ttgaaaattt
      721 tgtaatcggt tcaagtaatg aacaagcttt tatagcagtt caaacagtaa gtaaaaatcc
      781 tggaatttct tataatccat tattcattta tggtgaatct ggaatgggaa aaactcattt
      841 attaaaggct gcaaaaaact atattgaatc taatttttct gatctaaaag ttagttatat
      901 gagtggtgat gagtttgcaa gaaaagcagt tgatatatta caaaaaactc ataaagagat
      961 tgagcaattt aaaaatgaag tatgtcaaaa tgatgtatta attattgatg atgttcaatt
     1021 tttaagttat aaagaaaaaa ctaatgaaat attttttact atttttaata actttataga
     1081 aaatgataaa cagctgtttt tttcaagtga taaatctcct gaattattaa atggttttga
     1141 taatagatta attactagat ttaatatggg gttaagtatt gctattcaaa aactagataa
     1201 taaaacagca acagctatca ttaaaaaaga gattaaaaat caaaacatta aaacagaagt
     1261 gactaatgaa gcaattaatt ttatttctaa ttattattca gatgatgtta gaaaaattaa
     1321 aggaagtgtt tcaagattaa acttttgatc tcaacaaaat ccagaagaaa aagttattac
     1381 tatagaaata atttctgatc tatttagaga tatacctact tcaaaattag gtattttaaa
     1441 tgttaaaaaa attaaagaag ttgttagtga aaaatatggt atttcagtta atgctattga
     1501 tggaaaggct agaagtaaat caattgtaac agcaagacac atagcaatgt atttaacaaa
     1561 agaaatttta aatcacactt tagctcaaat tggagaagaa tttggtggta gagaccacac
     1621 aacagttatt aatgctgaaa gaaaaataga aatgatgtta aaaaaagaca aacaattaaa
     1681 aaagactgtt gatattttaa agaataaaat tttaacaaaa taataaaaat aactatttta
     1741 aatctatgtt tttataagtt gttcacaaat taacctcata ataagaataa tattttagaa
     1801 ataaattata gaaatagaaa tacaaaacat tccttaattt aatttaaatt aaattagaat
     1861 aaaaaactta tcttaaaagg agtaattatg aatttttcaa taaatagaat cgttttatta
     1921 gataatttat caaaagctgc caaagtaatt gattataaaa atgtaaatcc aagcttatca
     1981 ggtatttatt taaatgtttt aaatgatcaa gttaatgtta ttactactag tggtatactt
     2041 tcttttaaaa gtattttaaa caatcaaaat tcagatttag aagttaaaca agaaggtaaa
     2101 gttttattaa aacctaaata tgttttagaa atgttaagaa gactagatga tgaatttgta
     2161 gtattttcaa tggttgaaga taatgaatta attattaaaa ctaataattc agattttagt
     2221 ataggagttt taaattcaga agattatcct ttaattgggt ttagagaaaa aggaattgaa
     2281 tttaatttaa atcctaaaga agttaaaaaa actatttatc aagtttttgt ttcaatgaat
     2341 gaaaataaca aaaaattaat tcttacaggt ttgaatttaa aactaaataa taatcaagca
     2401 attttttcaa caactgattc atttagaatt agccaaaaga ttttagaaat tcaaagtgat
     2461 aataatgaag atattgacat aacaattcct tttaaaactg ctttagaatt acctaag
//