From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Synechococcus sp. phycocyanin alpha subunit (cpcD, cpcE, cpcF)
Date: 1 Sep 92 10:03:25 GMT
Distribution: bionet
Checksum: 63513     9

LOCUS       SYOCPCDEF    4371 bp ds-DNA             BCT       01-SEP-1992
DEFINITION  Synechococcus sp. phycocyanin alpha subunit (cpcD, cpcE, cpcF)
            genes, complete cds.
ACCESSION   M93569
KEYWORDS    phycocyanin alpha subunit; phycocyanobilin lyase.
SOURCE      Synechococcus sp. (individual_isolate PR6000, strain PR6) DNA.
  ORGANISM  Synechococcus sp.
            Prokaryotae; Gracilicutes; Oxyphotobacteria; Cyanobacteria;
            Chroococcales.
REFERENCE   1  (bases 1 to 4371)
  AUTHORS   Zhou,J., Gasparich,G.E., Stirewalt,V.L., de Lorimier,R. and
            Bryant,D.A.
  TITLE     The cpcE and cpcF genes of synechococcus sp. PCC 7002: Construction
            and phenotypic haracterization of interposon mutants
  JOURNAL   J. Biol. Chem. 267, 16138-16145 (1992)
  STANDARD  full staff_entry
FEATURES             Location/Qualifiers
     RBS             32..38
                     /gene="cpcD"
     CDS             43..285
                     /function="phycocyanin-associated, 9 kDa rod-linker
                     polypeptide; reduces rodlength variation"
                     /product="'CpcD'"
                     /gene="cpcD"
                     /codon_start=1
                     /translation="MLSQFANGTEAASRVFTYEVQGLRQTEETDNQEYAFRRSGSVFI
                     NVPYARMNQEMQRILRLGGKIVSIKPYTGATASDEE"
     stem_loop       352..388
     RBS             410..418
                     /gene="cpcE"
     CDS             422..1228
                     /function="phycocyanin alpha subunit phycocyanobilin lyase
                     (with CpcF subunit)"
                     /product="'CpcE'"
                     /gene="cpcE"
                     /codon_start=1
                     /translation="MSDWQMAEAWTLEEAIANIQQTEDTGKRYYAAWWFGKFRVQDER
                     AVNALLAALKDETDRSPDGGYPLRRNAAKALGKLGNLAAVQPLIESLESPDYYVRESA
                     AQSLEMLGDRQAIPALQALLAGGVAAAVKAEGKPHLVQPYEAVIEALGTIGATAAIAE
                     IEPFLDHEFAKIPYAALRALYQLTQEAHYAEQLMEALNGNQLQLRRSALLDLGAIGYV
                     PAGQAIAKAYAENSLKLISLKGILESHLQRTAETLDADGLQLLELMDSLL"
     CDS             1282..1899
                     /function="phycocyanin alpha subunit phycocyanobilin lyase
                     (with CpcE)"
                     /product="'CpcF'"
                     /gene="cpcF"
                     /codon_start=1
                     /translation="MTVDVLIRAVNNPTSAQDLVKNVAQLAATKDEQAIPTLVEVLKF
                     NNPGAAVAAVNGLINIGEAVVPYLLENVDGYNYGARAWMLRIFAGIGDPRALDLLIEA
                     ANKDFAFSVRRSAAKGLGNIQWHKVPDSEREVQQQKVCDCLFLALEDGEWVVRYGAIA
                     GLEGLSQAIPEARKIVIKNKLTEFLTTEPEAAIRARIQKAILSLP"
     stem_loop       1906..1936
     stem_loop       2002..2046
BASE COUNT     1094 a   1093 c   1074 g   1110 t
ORIGIN
        1 tttattgtac ataaatcttt tacggagttc caagaaagaa ccatgttgag tcaatttgcg
       61 aatggaacgg aagcggcttc gcgtgttttt acctacgaag tgcaagggct gcgccaaaca
      121 gaagaaacag acaatcaaga atacgccttt cgtcgcagtg gtagtgtttt tatcaatgtg
      181 ccctatgctc ggatgaatca agagatgcaa cggattttgc gtctaggcgg caagattgtt
      241 tcgattaaac cttatacggg tgcgactgct tctgacgagg aataattttg ttgtttcttt
      301 ctgtcgctga ataagcttaa taacattgtt gttttgaata ttttttctag acggggtagc
      361 agtgctttgg ttttagtgct gttgctccga cttgttgtgg gggctattta aaggagaatt
      421 tatgtcggac tggcagatgg ctgaggcctg gaccctagag gaggcgatcg ccaatatcca
      481 acaaacggaa gatacaggta aacgctatta tgcggcttgg tggtttggca agttccgggt
      541 gcaggatgag cgggccgtaa atgcgttgct agcagcgtta aaagatgaga cggatcggtc
      601 gccggatgga ggttatcctc tacgtcgaaa cgcagcaaag gcgttgggca agctgggcaa
      661 tctggcagcg gtgcaaccgt taattgagag tttagaaagt cctgattatt atgtgcggga
      721 gtcggcggcc caatcgttgg agatgttggg agatcggcag gcaattcctg cgttacaagc
      781 gctacttgca ggaggggttg cagcggcagt caaggctgag ggcaaacccc atttagtgca
      841 gccctatgaa gcggtgattg aggcgttagg gacgattggt gccacggcgg cgatcgccga
      901 gattgagcca tttttagatc atgaattcgc aaaaattccg tacgcagcac tacgggcctt
      961 atatcaactg acccaagaag cccattacgc tgaacagtta atggaagcct tgaatggtaa
     1021 ccagctacag ttacgacggt ctgcgctgtt agatttgggt gctatcggct atgttccggc
     1081 gggtcaggcg atcgccaaag cctatgccga aaatagctta aagttaattt ccctcaaggg
     1141 cattctcgaa tcccatttac aacggaccgc cgaaaccctc gatgcggacg gtttacaatt
     1201 gctcgaatta atggatagtc tgctctagga acctcataaa ccaaatccct acattttttg
     1261 cgatcgctaa tttctgaacc catgaccgtc gacgttttaa ttcgtgccgt taacaacccc
     1321 acttcagccc aagacctggt caaaaatgtg gcccaactcg ccgcaacaaa agatgaacaa
     1381 gccattccaa ctctggtaga agtgctgaaa tttaataatc cgggggcggc cgtcgcggcg
     1441 gtcaatggtc tgatcaatat tggcgaggcc gttgtgccct acctcctcga aaatgtcgat
     1501 ggctataact acggagcgcg ggcatggatg ctgcggatct ttgccggaat tggtgatccc
     1561 agagctttag acctgctaat tgaggcggcg aataaggatt ttgcctttag tgtgcggcgc
     1621 tctgcggcca aagggttagg gaatattcaa tggcataaag tgccagattc tgagcgagaa
     1681 gtgcagcagc aaaaagtctg tgattgtcta ttcctggccc ttgaagatgg agaatgggtg
     1741 gttcgctatg gggcgatcgc cggactagag ggtttgtcgc aagccattcc agaagctaga
     1801 aaaatcgtca tcaaaaacaa actcaccgaa tttctcacca cagaaccaga agccgcaatc
     1861 cgtgcccgaa tccaaaaagc aatcctgagt cttccgtgac atactcccga cgctgaatca
     1921 aagattacag cgcgggcttc ttaaatcgct ttaagaaatt cctgcttcaa agcctcccta
     1981 ctgggcgtag ctccacagga ttgaacggtc tgtccaaccg ctacacttcg acatgctagc
     2041 tgttcgagtc gttgtacgag tcacgatcga tcaggtgctt tgcgaattac ttgaccactg
     2101 gccacatctc gattggtggt gtagccacat tctgggcatt catgccgacg catggacaag
     2161 tcttttttca ccgctacacc acaggatggg cattcctgac tcgtgccatt agcagcaacc
     2221 ttttggaaat gcacaccacg cttaaagcag acccatctca atgtctcgag gaactgcccc
     2281 caaccagcat ccagacaatg cttccccagc attccttttg ccagtccttt taggttcaac
     2341 tcctccgcaa atatcatctc tgcttggtcg cagagatgat gggctaactt acggtgaaaa
     2401 tctttgcgtc ggttcgcaat tcgttcatag aatctggcga ctcgttgacg agctttttgc
     2461 tggttctttg aacctttctg ctttctggca acggcacggt tcagcacttt cagcctgtgt
     2521 tgtgcatcca caaagaatcg gggtctccca atcaactcgc cttctgatgt cgcaacaaaa
     2581 tgacttaacc ccacatcaat cccaatggca taaccaccaa tcgggggatc tggaatcttt
     2641 acgtcgcttt ggataactag ctggatatac caaccagaag cgcgtttaac tacccgtgct
     2701 tgcttcacct caaacccatc agggagcggg cggtgcatca cggtcttaac ccaaccaaat
     2761 acaggcaact taatttgatt gtcaccaatt gggtttttgc ccaactgcgg gaagctaaag
     2821 ctgcgaaaac gcttcttaaa ccgtgggaat ccgaacccac tattccacaa tgcattaaac
     2881 gccttttcca gcctgcccat cacatcctga agcacttgag aatgcaccgc ttgagaaaag
     2941 aattagtttt ccttgcggcg gtcaaattgc gcttttgact gtaataggtc ggcttttctg
     3001 aatccattgg gtaaataaat tcccgctgaa tactgcaagc attaatcggg cacttacggg
     3061 actgaatcca atcacgacgt tcagctaagg cgtagttata gacgcgcttg cacgtgtcca
     3121 acagctaagg cgtagttata gacgcgcttg cacgtgtcca accattcatc cattttgatg
     3181 gcttggtcgg cagtcggata gactctgtac tcgtaagtca ggttaatcat acctgtacca
     3241 tagtaagact tagggaaatc gtacactttt tctaagacgc ttaactagga gtttgctgct
     3301 tcccgctggt cagtcgctcg ctcctcatcc cgacgttgat atttcacata caacgcgggg
     3361 cttccccgct gttaagctaa gcaaaaaaat ccccctgacc caggaggatt tgtgggaatg
     3421 tcattctcca agcgaaacta cttcacaaat tggggcatca tatccgccgc cgtgaagatg
     3481 cctgttaaac cttgcttttg caaccaaacc cccgttttga ggtaaccgaa agcggggcca
     3541 caaacattgg cggccatgct agtttcatcc ccaagggtga acgtatgggt tgcaatcttg
     3601 ccttcaaagg tacggcccgt caattgcaca ttggtgctga ggggtttttt cggattgcgc
     3661 gtatctacca caccgccgac actaacttga tcacgggaac aaatcccggc caattccagc
     3721 atgagatcat cggcatgttc catattttcg agggaaataa tgccattggt ttcatccaac
     3781 agggcttcaa cctctgcatc gctcatggcc cgcgctttgt ccacatcata gccaggcatg
     3841 tgggcaatat cttcccggat ggtcgcccgg taagcttccc agttcgcaat acccaccccg
     3901 aagtgatttt aacgctgtga acctctgcat agctttgggc cgcgatcgcc gccgccgccg
     3961 tcaacaaacc aggcgtcgcg ccgcaacccg tcatgtaggt aatacctgcg tttttcacct
     4021 cgtccttgag ggagaggatt tgttccatcg cactggtgcg cttcagggca tccactaaca
     4081 ccccttgcca accagaagca ataaattgcc gggtcacatc agccataaac gtgttgggca
     4141 agttcggcag agcgaggaaa aaaccatcca cttgagcatt agcgatcaga tcggcaatac
     4201 tgttttgact gaggacgcca tattctggca catgacccac agaaccctgc tggttgtagg
     4261 tgctgatgca ggcttgggta tcaaggcctt caggattata gatataacct ttatggtcag
     4321 cagcggcgac gaggcgcatt tctgattttg gagctagtaa tcgggctgca g
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