HDTenEightyP/NetNews huggingface dataset:
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human immunodeficiency virus type 2 (FOENVAD2) envelope protein
Date: 18 Aug 92 10:01:45 GMT
Distribution: bionet
Checksum: 47579     2

LOCUS       HIVENVAD2     453 bp ss-RNA             VRL       18-AUG-1992
DEFINITION  Human immunodeficiency virus type 2 (FOENVAD2) envelope protein
            fragment.
ACCESSION   M87082
KEYWORDS    envelope protein.
SOURCE      Human immunodeficiency virus type 2 uncultured blood mononuclear
            cells (11/89 sample) RNA.
  ORGANISM  Human immunodeficiency virus type 2
            Viridae; ss-RNA enveloped viruses; Positive strand RNA virus;
            Retroviridae; Lentivirinae.
REFERENCE   1  (bases 1 to 453)
  AUTHORS   Gao,F., Yue,L., White,A.T., Pappas,P.G., Barchue,J., Hanson,A.P.,
            Greene,B.M., Sharp,P.M., Shaw,G.M. and Hahn,B.H.
  TITLE     Human infection by genetically-diverse SIVsm-related HIV-2 in West
            Africa
  JOURNAL   Nature (1992) In press
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     misc_feature    1..453
                     /note="PCR derived HIV-2 envelope fragment"
                     /gene="env"
                     /note="putative"
                     /citation=[1]
BASE COUNT      183 a     77 c     83 g    110 t
ORIGIN
        1 aacgtggtca aaagacaaca aaaattgttg cgactaaccg tctgggagac taaaaacctc
       61 cagactaaag tcactgctat caaaaagtac ctaaagaacc aggcacagct aaattcatag
      121 gaatgcgcgt tcagacaggt ctgccacacc actgtaccat ggccaaataa tagtctcaca
      181 cctaactgaa acaatataac ttggcagaag tagaaaagaa aggttaattt cctaaaggca
      241 aatataacac agatgttaaa gaaagcacaa attcaacagg agaaaaacat gtataagtta
      301 caaaaactaa ataactggga tatatttggc aactggtttg accttacttc ttggattaag
      361 tatatacagt atggagtctt catagtagta ggtataatac tgttaagaat aataatctat
      421 gtagtacaaa tgttagctaa gttaagtaag gga
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Arabidopsis thaliana recombination and DNA-damage resistance
Date: 12 Aug 92 10:00:25 GMT
Distribution: bionet
Checksum: 65234     3

LOCUS       ATHDRT112X    705 bp ss-mRNA            PLN       12-AUG-1992
DEFINITION  Arabidopsis thaliana recombination and DNA-damage resistance
            protein (DRT112) mRNA, complete cds.
ACCESSION   M98456
KEYWORDS    DNA-damage resistance protein; recombination protein.
SOURCE      Arabidopsis thaliana (strain Columbia) (library: Lambda-YES-R) cDNA
            to mRNA.
  ORGANISM  Arabidopsis thaliana
            Eukaryota; Plantae; Embryobionta; Magnoliophyta; Magnoliopsida;
            Dilleniidae; Capparales; Brassicaceae.
REFERENCE   1  (bases 1 to 705)
  AUTHORS   Pang,Q., Hays,J.B. and Rajagopal,I.
  TITLE     Two plant cDNAs from Arabidopsis thaliana that partially complement
            deficiencies in recombination and DNA-damage resistance in E. coli
            mutants lacking recombination-intermediate-resolution activities
  JOURNAL   Unpublished (1992)
  STANDARD  full staff_entry
FEATURES             Location/Qualifiers
     5'UTR           1..30
     CDS             31..534
                     /gene="DRT112"
                     /codon_start=1
                     /translation="MASVTSTTVPIPSFTGLKASTTKSSATVRTQTAAVASPKLTVKS
                     SLKNFGVAAVAPAASIALAGNAMAIEVLLGGGDGSLAFIPNDFSIAKGEKIVFKNNAG
                     YPHNVVFDEDEIPSGVDVAKISMDEQDLLNGAGETYEVALTEPGTYSFYCAPHQGAGM
                     VGKVTVN"
     3'UTR           535..705
BASE COUNT      185 a    163 c    167 g    190 t
ORIGIN
        1 taaactaacc tactgaaaag aatcttcatc atggcctcag taacctcaac caccgttcca
       61 atcccatctt tcaccggcct taaagcctcc accaccaaat catccgccac cgtcagaacc
      121 caaactgctg ctgttgcatc accgaagctt acagtgaagt catctctaaa gaacttcgga
      181 gtcgcggccg tagcgcctgc agcttcaatt gctttggccg gaaacgccat ggcaatagaa
      241 gttctcttgg gaggagggga tgggtcgtta gcttttattc ccaacgactt ctctatagct
      301 aaaggagaga agattgtgtt caagaacaac gctggatacc cacacaatgt tgtcttcgat
      361 gaagacgaga tcccaagtgg cgtcgacgtg gccaagatct cgatggacga gcaagatcta
      421 ctcaacggtg cgggagagac gtacgaggtt gctttgaccg agccagggac ttacagcttc
      481 tactgtgcgc cacatcaggg tgctggtatg gtcggtaaag tcaccgttaa ctaaaatgtg
      541 tgtgtgagta agtgagggag tctcggactc aattagaggc tcttctttca cattcttaca
      601 ttattacata aagtgttttg taattctaaa gagtgttgaa tcgaatggac cgtgattgtt
      661 gttgtactgt gactggttcc ataaatggtt tttcttggtt tatat
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human BTF3 protein homologue gene, complete cds.
Date: 20 Aug 92 10:02:28 GMT
Distribution: bionet
Checksum: 35513     3

LOCUS       HUMBTFB      1450 bp ds-DNA             PRI       20-AUG-1992
DEFINITION  Human BTF3 protein homologue gene, complete cds.
ACCESSION   M90354
KEYWORDS    BTF3 protein homologue.
SOURCE      Homo sapiens DNA.
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 1450)
  AUTHORS   Kanno,M., Chalut,C. and Egly,J.-M.
  TITLE     Genomic structure of the putative BTF3 transcription factor
  JOURNAL   Gene 117, 219-228 (1992)
  STANDARD  full staff_entry
FEATURES             Location/Qualifiers
     CDS             join(553..649,950..1188)
                     /note="putative"
                     /product="BTF3 homologue"
                     /gene="BTF3 homologue"
                     /note="putative"
                     /codon_start=1
                     /translation="MKETIMNQKLTKRQAEVHTGRKGTAHRKKVVHTTAEDKKFQFSL
                     KKLGVNNIPGIEEVNMFTHQGTVIHFNNPEVQASLAANTFTMTGHAETKQLTEMLLSI
                     DHKPVLQMV"
BASE COUNT      465 a    311 c    289 g    385 t
ORIGIN
        1 aagcttttag ttccctttaa tcataaaagc cacttgctaa ctaaaactag agatagctca
       61 agctatctga ttttaaaggc ttagtctcaa tgtgtccctt ccctgaaatc ccagtagagt
      121 agccaattgt ctgaaacccg cttggattta gcaatgaaac acctcagtcc tggccaaacc
      181 aagacagtgg gtctcaggaa acattcttca tcctaataaa ggcaaattaa ctacagttga
      241 cccttgaaca acatgggggt tatgggtgct gacttcccat gcagtaaaaa atctgggtat
      301 aactttcgat tccacaaaaa ctttgctaat agccttaact gttaactgga agccttacca
      361 ataacacata cagttgatta acacatatat tgtatgtatt atattacaat aaattaagct
      421 agagaaaaga aaatgtattc cttttgctgc catcctgcct ccctgtgtgc ttctaatctc
      481 agctggtctc acccgggact ccccaagtgc caaccctagt cccccacgca gccccttttc
      541 cactcagata agatgaaaga aacaatcatg aaccaaaaac tcaccaaacg gcaagcagaa
      601 gtgcacactg gtcggaaagg aactgctcac aggaaaaagg tggttcacag catctgagac
      661 gctgtgtttg agggcaagta ggcccctttg acacctttgg tgttgaactc atgaggtttt
      721 gaatgtccag ggacattggc caatatcaaa agaactttaa aagtcagttt ggtaaggtac
      781 ttcttgactt cagggacaaa acagcaatgg aaccaatcca gaaaaagggt tctcattgtc
      841 caggccttct ggtacaacca aaagactggc agctggcatt tatcttgtcc cttcaaggct
      901 cagaggttaa cggttttata cataagggta gtcctgatca taaacctagc gacagcagag
      961 gataaaaaat ttcagttctc cttaaagaaa ttaggggtaa acaatatccc tggtattgaa
     1021 gaggtgaata tgtttacaca ccaaggaaca gtgattcact ttaacaaccc tgaagttcag
     1081 gcatcgctgg cagcaaacac tttcaccatg acaggccatg ctgagacaaa gcagctgaca
     1141 gaaatgctac tcagcatcga tcataaacca gtgctgcaga tggtctgact agttcaaaga
     1201 gactggctaa cactgcccaa acaatctgtg ggtggaaaag caccacttgc tactggagag
     1261 gatgatgaag ttctagatct tgtggagaat tctgatgagg cttccaacaa tgaggcaaac
     1321 tgaattaagt caacttctga agaagataaa acttgaagta gttactgaga gctgctgttt
     1381 tatgttatga ctgcttttta aaaatgtttt tgtttacaga tcttaataaa atctagatct
     1441 ctaatatttt
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human chromosome 4 sequence-tagged site STS4-398
Date: 21 Aug 92 10:09:51 GMT
Distribution: bionet
Checksum: 05577     3

LOCUS       HUM4STS398    391 bp ds-DNA             PRI       21-AUG-1992
DEFINITION  Human chromosome 4 sequence-tagged site STS4-398
ACCESSION   L00838
KEYWORDS    STS; human chromosome 4; sequence tagged site.
SOURCE      Homo sapiens, clone p4-403 from a chromosome 4 plasmid library;
            vector pBluescript II KS + (Stratagene)
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 391)
  AUTHORS   Goold,R.D., diSibio,G., Dugaiczyk,A., Smith,K.A., Xu,H.,
            Magrane,G., Lang,D.B., Cox,D.R., Masters,S.B. and Myers,R.M.
  TITLE     Sequence-Tagged Sites for Human Chromosome 4
  JOURNAL   Unpublished (1992) see COMMENT for author address
  STANDARD  full automatic
COMMENT     PCR components: 25 ng of human genomic DNA, 10 pmol of each
            oligonucleotide, 200 micro-M dNTPs, 0.25 U Taq  polymerase (Cetus)
            in 10 micro-l of 50 mM KCl-20 mM  Tris-HCl, pH 8.3 (at room temp),
            2.5 mM MgCl-2.  Initial denaturation at 94degC for 1.5 min, then 30
            cycles of  94degC for 15 sec, 62degC for 23 sec, and 72degC for 30
            sec, followed by a final extension at 72degC for 3.5 min, using a
            Perkin-Elmer 9600 thermocycler. PCR-amplified product size 150 bp.
            Sequence submitted by:
            Human Genome Mapping Center
            Box 0925
            University of California San Francisco
            San Francisco, CA 94143-0925  USA
            Phone: (415) 502-1612   Fax: (415) 476-8391
            e-mail: hgmcprobes@cgl.ucsf.edu
FEATURES             Location/Qualifiers
     misc_feature    47..71
                     /label=Primer1
     misc_feature    complement(173..196)
                     /label=Primer2
BASE COUNT      151 a     71 c     73 g     90 t      6 others
ORIGIN
        1 gatcaatcta aaattacctt tgttagagaa aattaggtta aaaatagcag aaacagacat
       61 tgtaagaaag attccttttt cagaactaaa attcctggtc cctaaaactg acacatacta
      121 tgtatttcag tggaccccta aatgtggctg agccatgggt cttgggtctt ggtttgggaa
      181 ctgatagtct gcattganat cacacacaca cagacacaca cacacacaca cacacacata
      241 cacacacaga aacagagaga gagagagaga gagagagaga gagagagaat atctccaagt
      301 anttcttaga agtattaaat cantccctag tatnataata ggacacataa aagactaacc
      361 nggtaggtaa aatnctctgg gttaaaaaaa t
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Mouse T cell receptor rearranged alpha--chain variable region,
Date: 15 Aug 92 10:02:38 GMT
Distribution: bionet
Checksum: 33766     2

LOCUS       MUSTCRA4CX    426 bp ss-mRNA            ROD       15-AUG-1992
DEFINITION  Mouse T cell receptor rearranged alpha--chain variable region,
            N_region, joining region, and constant region mRNA.
ACCESSION   M98574
KEYWORDS    T-cell receptor; alpha-subunit receptor; constant region;
            joining region; variable region.
SOURCE      Mus musculus (strain BALB/cKh) adult spleen cDNA to mRNA.
  ORGANISM  Mus musculus
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Rodentia; Myomorpha; Muridae; Murinae.
REFERENCE   1  (bases 1 to 426)
  AUTHORS   Solheim,J.C., Martinko,J.M., Alexander-Miller,M. and Connolly,J.M.
  TITLE     Biased T Cell Receptor Usage by Ld-restricted Tum-Peptide-specific
            Cytotoxic T Lymphocyte Clones
  JOURNAL   Unpublished (1992)
  STANDARD  full staff_entry
BASE COUNT      114 a    129 c     85 g     98 t
ORIGIN      Chromosome 14
        1 gtcactctca ccgaggggtt gcctgtgatg ctgaactgca cctatcagac tgcttactca
       61 actttccttt tctggtatgt gcaacatctc aatgaagccc ctaaactact cctgaagagc
      121 tccacagaca acaagaggac cgagcaccaa gggttccacg ccactctcca taagagcagc
      181 agctccttcc atctgcagaa gtcctcagcg cagctgtcag actctgccct gtactactgt
      241 gctctgagtg tttctggagg aagcaatgca aagctaacct tcgggaaagg cactaaactc
      301 tctgttaaat caaacatcca gaacccagaa cctgctgtgt accagttaaa agatcctcgg
      361 tctcaggaca gcaccctctg cctgttcacc gactttgact cccaaatcaa tgtgccgaaa
      421 accatg
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human chromosome 4 sequence-tagged site STS4-41
Date: 21 Aug 92 10:10:02 GMT
Distribution: bionet
Checksum: 64707     3

LOCUS       HUM4STS41     536 bp ds-DNA             PRI       21-AUG-1992
DEFINITION  Human chromosome 4 sequence-tagged site STS4-41
ACCESSION   M95304
KEYWORDS    STS; human chromosome 4; sequence tagged site.
SOURCE      Homo sapiens, clone L4-21 from the C25 lambda library (Pritchard,
            Genomics 4, 408, 1989); vector - Lambda Dash II (Stratagene)
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 536)
  AUTHORS   Goold,R.D., diSibio,G., Dugaiczyk,A., Smith,K.A., Xu,H.,
            Magrane,G., Lang,D.B., Cox,D.R., Masters,S.B. and Myers,R.M.
  TITLE     Sequence-Tagged Sites for Human Chromosome 4
  JOURNAL   Unpublished (1992) see COMMENT for author address
  STANDARD  full automatic
COMMENT     PCR components: 25 ng of human genomic DNA, 40 pmol of each
            oligonucleotide, 200 micro-M dNTPs, 0.25 U Taq  polymerase (Cetus)
            in 10 micro-l of 50 mM KCl-20 mM  Tris-HCl, pH 8.3 (at room temp),
            2.5 mM MgCl-2.  Initial denaturation at 94degC for 1.5 min, then 30
            cycles of  94degC for 15 sec, 66degC for 23 sec, and 72degC for 30
            sec, followed by a final extension at 72degC for 3.5 min, using a
            Perkin-Elmer 9600 thermocycler. PCR-amplified product size 205 bp.
            Sequence submitted by:
            Human Genome Mapping Center
            Box 0925
            University of California San Francisco
            San Francisco, CA 94143-0925  USA
            Phone: (415) 502-1612   Fax: (415) 476-8391
            e-mail: hgmcprobes@cgl.ucsf.edu
FEATURES             Location/Qualifiers
     misc_feature    65..91
                     /label=Primer1
     misc_feature    complement(244..269)
                     /label=Primer2
BASE COUNT      112 a    118 c     94 g    134 t     78 others
ORIGIN
        1 accnccncng ngtattgnat ggcgtgnctt caanngntnc ttccggngaa gtnattatgn
       61 gtntgagtgt atgtatgtgt gtgtgtgtgt ctgtgtgtgt gttatgacat tatacctgga
      121 gagaaaaaca antcaccaag gctgantcca gaagaagntt nggnatccat atgncactca
      181 cctnaatcat tttcaantca gnctgccctt nntttttnng acncagtcaa ggagctantt
      241 gtntcttgtc aatgccatag ataccctcaa agncctntaa angatgctct ggncttatgn
      301 aggctgacnc atangacant cactngnggn ggntcncnnn cacattggnt ganttcaaac
      361 acatcagatc ntatccaccc gcgttnnncg nctgtcctng cgctaccatn gtgttaancg
      421 cttcccnnca attagcccgc aagaaanccc tntctttnna tccncgctca nnntctgtac
      481 cctnccaaan aatnggtgga ctagcncnct nnatacccnc nnatatnatc tcgntc
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human Ig germline H-chain gene V region, partial cds.
Date: 22 Aug 92 10:00:38 GMT
Distribution: bionet
Checksum: 51803     2

LOCUS       HUM4IGVH      434 bp ds-DNA             PRI       22-AUG-1992
DEFINITION  Human Ig germline H-chain gene V region, partial cds.
ACCESSION   M99400
KEYWORDS    immunoglobulin heavy chain subgroup VH-III;
            immunoglobulin heavy chain variable region.
SOURCE      Homo sapiens (individual_isolate LS) blood DNA.
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 434)
  AUTHORS   Adderson,E.E., Carroll,W.L. and Shackelford,P.G.
  TITLE     The human VH3b gene subfamily is highly polymorphic
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     CDS             join(1..13,117..434)
                     /product="immunoglobulin heavy chain variable region"
                     /gene="IGHV@"
                     /codon_start=1
                     /translation="AILKGVQCEVQLVESAGGLVQPGGSLRLSCAASGFTCSNAWMSW
                     VRQAPGKGLEWVGCIKSKANGGTTDYAAPVKGRFTISRDDSKNTLYLQMISLKTEDTA
                     VYYCTTGT"
                     /partial
     sig_peptide     1..13
                     /gene="IGHV@"
                     /codon_start=1
                     /partial
     intron          14..116
                     /gene="IGHV@"
BASE COUNT      108 a     85 c    137 g    104 t
ORIGIN
        1 gctattttaa aaggtgattt atggagagct agagagattg agtgtgagtg gacatgagtg
       61 agagaaacag tggatatgtg tggcagtttc tgaccttagt gtctctgtgt ttgcaggtgt
      121 ccagtgtgag gtgcagctgg tggagtctgc gggaggcttg gtacagcctg gggggtccct
      181 tagactctcc tgtgcagcct ctggattcac ttgcagtaac gcctggatga gctgggtccg
      241 ccaggctcca gggaaggggc tggagtgggt tggctgtatt aaaagcaaag ctaatggtgg
      301 gacaacagac tacgctgcac ctgtgaaagg cagattcacc atctcaagag atgattcaaa
      361 aaacacgctg tatctgcaaa tgatcagcct gaaaaccgag gacacggccg tgtattactg
      421 taccacaggc acag
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human gene for branched chain alpha-keto acid dehydrogenase (EC
Date: 20 Aug 92 10:02:11 GMT
Distribution: bionet
Checksum: 35975     3

LOCUS       HUMBCKD07     128 bp ds-DNA             PRI       20-AUG-1992
DEFINITION  Human gene for branched chain alpha-keto acid dehydrogenase (EC
            1.2.4.4) E-1-beta subunit, exon 7.
ACCESSION   D90388
KEYWORDS    branched chain alpha-keto acid dehydrogenase;
            maple syrup urine disease.
SEGMENT     7 of 10
SOURCE      Human adult peripheral blood, white blood cell DNA.
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 16 to 113)
  AUTHORS   Matsuda,I., Asaka,J., Akaboshi,I., Endo,F., Mitsubuchi,H. and
            Nobukuni,Y.
  TITLE     Maple syrup urine disease: Complete primary structure of the
            E-1-beta subunit of human branched chain alpha-ketoacid
            dehydrogenase complex deduced from the nucleotide sequence and a
            gene analysis of patients with this disease
  JOURNAL   J. Clin. Invest. 86, 242-247 (1990)
  STANDARD  simple staff_review
REFERENCE   2  (bases 1 to 128)
  AUTHORS   Mitsubuchi,H., Nobukuni,Y., Endo,F. and Matsuda,I.
  TITLE     Structural organization and chromosomal localization of the gene
            for the E-1-beta subunit of human branched chain alpha-keto acid
            dehydrogenase
  JOURNAL   Unpublished (1991)
  STANDARD  full staff_review
COMMENT     These data kindly submitted in computer readable form by:
            Hiroshi Mitsubuchi
            Department of Pediatrics
            Kumamoto University Medical School
            1-1-1 Honjo, Kumamoto 860
            Japan
            Phone:  096-344-2111 x5654
            Fax:    096-366-3471
FEATURES             Location/Qualifiers
     intron          <1..15
                     /note="intron 6"
     exon            16..113
                     /number=7
                     /note="E-1-beta subunit"
     intron          114..>128
                     /note="intron 7"
BASE COUNT       34 a     29 c     33 g     32 t
ORIGIN      Chromosome 6.
        1 ctgttctgta tttagcggaa gaagtcccta tagaaccata caacatccca ctgtcccagg
       61 ccgaagtcat acaggaaggg agtgatgtta ctctagttgc ctggggcact caggtgagta
      121 gcattgat
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Lentinus dactyloides ribosomal RNA (55LR0R) large subunit, partial.
Date: 19 Aug 92 10:01:58 GMT
Distribution: bionet
Checksum: 25244     2

LOCUS       LENRRLSAX     230 bp ss-rRNA            RNA       19-AUG-1992
DEFINITION  Lentinus dactyloides ribosomal RNA (55LR0R) large subunit, partial.
ACCESSION   M98666
KEYWORDS    large subunit ribosomal RNA.
SOURCE      Lentinus dactyloides (library: Duke culture collection 785) cDNA to
            rRNA.
  ORGANISM  Lentinus dactyloides
            Eukaryota; Fungi; Eumycota; Basidiomycotina; Hymenomycetes;
            Agaricales; Tricholomataceae.
REFERENCE   1  (bases 1 to 230)
  AUTHORS   Hibbett,D.S. and Vilgalys,R.
  TITLE     Phylogenetic relationships of the basidiomycete genus Lentinus
            inferred from molecular and morphological characters
  JOURNAL   Unpublished (1992)
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     rRNA            <1..>230
                     /note="Aligns to region between positions 70 to 306
                     inSaccharomyces cerevisiae large subunit nuclear rRNA."
                     /product="large subunit ribosomal RNA"
                     /citation=[1]
BASE COUNT       58 a     47 c     66 g     59 t
ORIGIN
        1 attcccctag taactgcgag tgaagcggaa aagctcaaat ttaaaatctg gtggtctttg
       61 gccatccgag ttgtaactag agaagtgtta tccgcgctgg accgtgtata atcccctgga
      121 atggggcgtc atagagggtg agaatcccgt ctttgacacg gactaccagg gcttgtgatg
      181 cactctcaac gagtcgagtt gtttgggaat gcagctcaaa atgggtggta
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human chromosome 4 sequence-tagged site STS4-190
Date: 21 Aug 92 10:07:33 GMT
Distribution: bionet
Checksum: 53862     3

LOCUS       HUM4STS190    429 bp ds-DNA             PRI       21-AUG-1992
DEFINITION  Human chromosome 4 sequence-tagged site STS4-190
ACCESSION   L00898
KEYWORDS    STS; human chromosome 4; sequence tagged site.
SOURCE      Homo sapiens, clone C4-178 from Los Alamos National Laboratory
            chromosome 4 cosmid library, 2C4; vector SuperCos 1 (Stratagene)
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 429)
  AUTHORS   Goold,R.D., diSibio,G., Dugaiczyk,A., Smith,K.A., Xu,H.,
            Magrane,G., Lang,D.B., Cox,D.R., Masters,S.B. and Myers,R.M.
  TITLE     Sequence-Tagged Sites for Human Chromosome 4
  JOURNAL   Unpublished (1992) see COMMENT for author address
  STANDARD  full automatic
COMMENT     PCR components: 25 ng of human genomic DNA, 10 pmol of each
            oligonucleotide, 200 micro-M dNTPs, 0.25 U Taq  polymerase (Cetus)
            in 10 micro-l of 50 mM KCl-20 mM  Tris-HCl, pH 8.3 (at room temp),
            2.5 mM MgCl-2.  Initial denaturation at 94degC for 1.5 min, then 30
            cycles of  94degC for 15 sec, 62degC for 23 sec, and 72degC for 30
            sec, followed by a final extension at 72degC for 3.5 min, using a
            Perkin-Elmer 9600 thermocycler. PCR-amplified product size 182 bp.
            Sequence submitted by:
            Human Genome Mapping Center
            Box 0925
            University of California San Francisco
            San Francisco, CA 94143-0925  USA
            Phone: (415) 502-1612   Fax: (415) 476-8391
            e-mail: hgmcprobes@cgl.ucsf.edu
FEATURES             Location/Qualifiers
     misc_feature    77..100
                     /label=Primer1
     misc_feature    complement(235..258)
                     /label=Primer2
BASE COUNT      110 a     74 c    107 g    121 t     17 others
ORIGIN
        1 tntgcnttnt nnntngnntc tcnaagtgct gggattacag gcttgagcca ccgcgtctgg
       61 cccaaaagtg attttttaaa atcactctag ggtggtcctt gtaaggctct aactctccat
      121 gatgaccgtg tagtttggtt tgacctaata cctgttgctg gggagacaag accagactag
      181 gaaagtgtac tttgtatgtg acaagctgaa taggttgttg acagattttt acaaggacgc
      241 tgtcaaacat aattggaaac attgtggcag agctgttgat tcttgggaaa tgacacctgc
      301 anagaggtta cttgtcaatc tcaagccacg tggttctnct atagtaagag gaaacanaac
      361 ttcctgtggg gnaaaaatgg cagagctttt aaagggagaa ggctgttngg nagccacggt
      421 ncatttttt
//