From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: methylmalonate-semialdehyde dehydrogenase [rats, liver, mRNA, 2059
Date: 3 Jan 93 22:20:38 GMT
Distribution: bionet
Checksum: 39359 5

LOCUS       S114130      2059 bp    mRNA            ROD       24-NOV-1992
DEFINITION  methylmalonate-semialdehyde dehydrogenase [rats, liver, mRNA, 2059
            nt]
ACCESSION   S45381
KEYWORDS    .
SOURCE      rats liver
  ORGANISM  Unclassified.
            Unclassified.
REFERENCE   1  (bases 1 to 2059)
  AUTHORS   Kedishvili,N.Y., Popov,K.M., Rougraff,P.M., Zhao,Y., Crabb,D.W. and
            Harris,R.A.
  TITLE     CoA-dependent methylmalonate-semialdehyde dehydrogenase, a unique
            member of the aldehyde dehydrogenase superfamily. cDNA cloning,
            evolutionary relationships, and tissue distribution.
  JOURNAL   J. Biol. Chem. 267, 19724-19729 (1992)
  STANDARD  full automatic
COMMENT     This entry [NCBI gibbsq 114130] was created by the journal scanning
            component of NCBI/GenBank at the National Library of Medicine.
            This sequence comes from Fig. 2.
FEATURES             Location/Qualifiers
     CDS             82..1689
                     /note="2-methyl-3-oxopropanoate:NAD+ oxidoreductase
                     (CoA-propanoylating);  MMSDH;  For the protein sequence
                     (NCBI gibbsq 114132):  Method: conceptual translation
                     supplied by author.  This sequence comes from Fig. 2.  The
                     authors begin their numbering at -32.  Zero is not present
                     in the numbering."
                     /product="methylmalonate-semialdehyde dehydrogenase"
                     /codon_start=1
                     /translation="MAAAVAAAAAVRSRILQVSSKVNSTWYPASSFSSSSVPTVKLFI
                     DGKFVESKSDKWIDIHNPATNEVVGRVPQSTKAEMEAAVAACKRAFPAWADTSILSRQ
                     QVLLRYQQLIKENLKEIARLITLEQGKTLADAEGDVFRGLQVVEHACSVTSLMLGETM
                     PSITKDMDLYSYRLPLGVCAGIAPFNFPAMIPLWMFPMAMVCGNTFLMKPSERVPGAT
                     MLLAKLLQDSGAPDGTLNIIHGQHEAVNFICDHPDIKAISFVGSNQAGEYIFERGSRN
                     GKRVQANMGAKNHGVVMPDANKENTLNQLVGAAFGAAGQRCMALSTAVLVGEAKKWLP
                     ELVERAKNLRVNAGDQPGADLGPLITPQAKERVCNLIDSGAKEGASILLDGRKIKVKG
                     YENGNFVGPTIISNVKPSMTCYKEEIFGPVLVVLETETLDEAIKIVNDNPYGNGTAIF
                     TTNGAIARKYAHMVDVGQVGVNVPIPVPLPMFSFTGSRSSFRGDTNFYGKQGIQFYTQ
                     LKTITSQWKEEDATLSSPAVVMPTMGR"
BASE COUNT      538 a    498 c    509 g    514 t
ORIGIN      
        1 ggcaggagaa gtgatctgtt atctcacctg gcacatgaag cccattcctg gaactatgca
       61 aagggcagag tctggtgagc catggcggcg gcagtggcgg cagcagcagc agtgcggtcc
      121 cggatcctgc aggtttcttc taaagtaaac tccacatggt atccagcatc ttctttctct
      181 tcatcatcag tgccaactgt aaagctcttc attgatggga aattcgttga atccaaaagt
      241 gacaaatgga ttgacatcca caacccagcc accaatgagg ttgttggtcg ggttcctcag
      301 tccaccaaag ctgaaatgga ggcagccgta gccgcctgca aacgcgcttt ccctgcgtgg
      361 gcagacacat ctattttaag ccggcagcag gtcctgcttc gttatcagca actcattaaa
      421 gaaaacctga aagaaatcgc caggttaatc acattggaac aagggaagac tctggctgat
      481 gctgaaggag atgtgttccg aggccttcag gtggttgagc atgcctgcag tgtgacgtcc
      541 ctcatgctgg gagagaccat gccatctatt accaaagaca tggaccttta ttcctaccgc
      601 ctgcctctgg gagtgtgtgc aggcatcgca ccatttaatt ttcctgccat gatccccctt
      661 tggatgtttc ccatggctat ggtctgtgga aatactttcc taatgaaacc atcagagcga
      721 gtacctggag caaccatgct tcttgctaag ttgcttcagg attctggtgc cccggacgga
      781 acactgaaca tcatccatgg acaacatgaa gctgtaaatt ttatttgcga tcatccagac
      841 atcaaggcaa tcagctttgt gggatccaac caggcgggag agtacatctt cgaaagaggg
      901 tcaagaaatg gcaagagggt tcaagccaat atgggagcca agaaccatgg ggtagtcatg
      961 ccagatgcca ataaggaaaa taccctcaac cagctggttg gggcagcatt tggagctgct
     1021 gggcagcgct gcatggctct ttcaacagcc gtccttgtgg gagaagctaa gaagtggctg
     1081 ccagaactgg tagagcgtgc caagaatctc cgcgtcaatg caggagatca gcctggagct
     1141 gatcttggtc ccctgatcac cccccaggcc aaagagagag tctgcaatct gattgatagt
     1201 ggagcaaaag agggggcttc catccttctg gatgggcgga aaattaaagt taagggctac
     1261 gaaaacggta actttgttgg accaaccatc atctccaatg ttaagccaag tatgacttgt
     1321 tacaaagagg agatttttgg tccagttctt gtggtcttag agacagaaac cctggatgaa
     1381 gccatcaaga ttgttaatga caacccatat ggaaacggga ctgctatctt taccaccaac
     1441 ggagccatag ctcggaaata cgctcacatg gtggatgtgg gacaggtggg cgtgaatgtc
     1501 cccattccag tgcctttgcc aatgttttcc ttcaccggct ctcgatcttc cttcagggga
     1561 gacaccaatt tctatggcaa acagggcatc cagttttaca ctcagttaaa gactatcaca
     1621 tctcagtgga aggaagaaga tgctactctt tcctcacctg cagtggtcat gcctaccatg
     1681 gggcgttaga acgttcaaga ctccatccag cctgagtaac ttcccactgt ccttggcaat
     1741 cttcgtctgc catctttgct caaaacagat ccatgaggat ggagtatatt gcaatgaaat
     1801 tctttctcag aactcgcaat cccttcaaag ttatagggag tacaggataa caccaacgcc
     1861 aggccttcac ttgttctcca tacttctcta cggtttttga ggttaactgt tgttaagcgt
     1921 gaagtgtttg cctagaataa gagcttagaa ctgtcttcta acaattcttt tctgatgact
     1981 tggagggaca acgtaggggc ccggtccttg gcaatcttcg tctgccatct ttgctcaaaa
     2041 acagtccatg aggatggag
//