From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Dichelobacter nodosus vapA, B, C, D genes, complete cds.
Date: 31 Dec 92 01:34:14 GMT
Distribution: bionet
Checksum: 06739 7

LOCUS       DIHVAPGENE   2262 bp ds-DNA             BCT       17-DEC-1992
DEFINITION  Dichelobacter nodosus vapA, B, C, D genes, complete cds.
ACCESSION   M74565
KEYWORDS    vapA gene; vapB gene; vapC gene; vapD gene.
SOURCE      Dichelobacter nodosus DNA.
  ORGANISM  Dichelobacter nodosus
            Prokaryotae; Gracilicutes; Scotobacteria; Anaerobic gram-negative
            straight, curved and helical rods; Cardiobacteriaceae.
REFERENCE   1  (bases 1 to 2262)
  AUTHORS   Katz,M.E. and Rood,J.I.
  TITLE     Molecular characterization of a genomic region associated with
            virulence in Dichelobacter nodosus
  JOURNAL   Infect. Immun. 60, 4586-4592 (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     -35_signal      40..45
                     /gene="vapD"
                     /note="putative"
     -10_signal      63..68
                     /gene="vapD"
     RBS             106..112
                     /gene="vapD"
     terminator      448..484
                     /gene="vapD"
                     /citation=[1]
                     /note="putative"
     RBS             complement(937..941)
                     /gene="vapC"
                     /note="putative"
     RBS             complement(1163..1168)
                     /gene="vapB"
     -10_signal      complement(1191..1196)
                     /gene="vapC"
     -10_signal      complement(1209..1214)
                     /gene="vapB"
     -35_signal      complement(1213..1218)
                     /gene="vapC"
     -35_signal      complement(1231..1236)
                     /gene="vapB"
     terminator      1417..1445
                     /gene="vapA"
     repeat_unit     1437..1463
                     /gene="vapA"
     RBS             complement(1807..1813)
                     /gene="vapA"
     -10_signal      complement(1837..1842)
                     /gene="vapA"
     -35_signal      complement(1860..1865)
                     /gene="vapA"
     repeat_region   2165..2191
     repeat_region   2199..2231
     CDS             119..400
                     /gene="vapD"
                     /evidence="EXPERIMENTAL"
                     /citation=[1]
                     /product="VapD"
                     /codon_start=1
                     /translation="MYAIAFDLEIAELKKHYGEPYNGAYLEIGKELKAIGFEWTQGSV
                     YLSNDNNLATVYRAISTLSKIDWFKSSVRDIRAFKVEDWSDFTAIVKGA"
     CDS             complement(522..929)
                     /gene="vapC"
                     /citation=[1]
                     /note="putative"
                     /product="VapC"
                     /codon_start=1
                     /translation="MSIKLMLDTNICIYIINRKPPYIAEKFSRYEIGDIGISNITACE
                     LAFGVEKSGSAKNKTALTKFLAPLSILPFDKQAIWHYARIRQSLQNRGTPIGALDMLI
                     ASHALALDITLVTNNTKEFERVDGLMLDNWVID"
     CDS             complement(926..1156)
                     /gene="vapB"
                     /citation=[1]
                     /note="putative"
                     /product="VapB"
                     /codon_start=1
                     /translation="MKVAKVFTTGRSQAVRIPKAFQFDTKEVIIQRFGNGILLIPKNS
                     DWQGFLEALNEFEPDFKIEREPQIEQVREDFL"
     CDS             complement(1492..1800)
                     /gene="vapA"
                     /citation=[1]
                     /note="putative"
                     /product="VapA"
                     /codon_start=1
                     /translation="MAKMKNPPHPGLMLKVMYLEPLGLNITETAEKLDMPRSALSEIV
                     NAKRAISPEVAVKLEKAFPKHSASFWLRAQAGYALSRVNPHCADKVKPIKTSPLMESV
                     "
     CDS             complement(1813..2172)
                     /citation=[1]
                     /note="putative;  ORF 118"
                     /codon_start=1
                     /translation="MPRLLVALSLNPVTLRDTIPPFFQGTSMIKSFAHKGLKRFFEDD
                     DTRGIQPHHAEKLRVLLYAMDIAETIQELDIEGAELHRLKGKLKTHWSLKVNGNWRVI
                     FEFKDGNAYIVDYVDYH"
BASE COUNT      681 a    457 c    456 g    668 t
ORIGIN      
        1 gatcgcatta aaagcgcagg attttcctta tctttttgat tgaaactcaa ttattgataa
       61 cagataattt gtgcaggaga aatatctctt ccatttagac aacatgagga atttaaagat
      121 gtacgcaatt gcttttgatt tagaaattgc cgaattaaaa aaacactatg gcgaaccgta
      181 caatggcgca tacttagaaa ttggtaaaga acttaaagca atcggatttg aatggacaca
      241 agggagcgtt tatctatcta atgataacaa cctcgcaacg gtttaccgtg caattagcac
      301 cctgtctaag attgattggt ttaaaagttc cgtaagagac attagagcat tcaaagttga
      361 ggattggtca gattttaccg caatagtgaa aggtgcatga tcatctaaac gcccagattc
      421 aagcggaggc atggactaag tatcctccaa aaaatatgaa gcagccaaat aggctgcttt
      481 ttttattgtc taaaaagtca taaggttatg gtgggagtat gtcaatctat cacccaatta
      541 tctaacatca acccatcaac gcgctcaaat tctttggtat tatttgttac caacgtgata
      601 tcaagcgcca atgcatggct agcgataagc atatctaaag ccccgatagg ggtaccgcgg
      661 ttttgcagtg attgccggat tcgtgcgtaa tgccaaatag cttgtttatc aaaaggcaat
      721 attgatagtg gtgccaaaaa ttttgttaat gcggttttgt tttttgctga acctgatttt
      781 tctacgccaa acgcaagctc acaagcggta atgttagata taccaatatc gcctatttca
      841 tatcgcgaaa atttttcggc aatatatggc ggttttcgat tgatgatata aatgcagata
      901 ttggtatcaa gcattaattt gatactcata aaaagtcttc ccgcacttgt tctatttgcg
      961 gctcacgctc aattttaaaa tcaggctcaa actcattgag tgcttccaga aaaccttgcc
     1021 aatccgaatt tttagggatt aataaaattc cgttgccgaa acgctgaata attacctctt
     1081 ttgtatcaaa ttggaacgcc ttaggaatgc gaaccgcttg cgagcgcccc gtagtaaaga
     1141 ctttggctac tttcatgata tgcctccttt tcattgtgat atatcaatgc cgtatatcat
     1201 actatcgaat tttagcaagg tagtttttta ttcgaaaaag tcatcgaaaa cggatagcgc
     1261 ctcattggcg tttagagccg ttattttatt tgggggtacc aaaaccatta ccgataacca
     1321 tcgataatgc gccagcgcat ctgatgagcg ctttatcaaa ataaaggaat tataaagatg
     1381 agccataatt tcaatatgaa ctttgaccca gaagttaaga aaataccgcg atgagccgcg
     1441 gcattggaag attaagtcat gagcatgagc gggaatatat ttttccgctt gttatacgga
     1501 ctccatcagg ggagaagttt taatgggttt cactttatcg gcacaatggg gattgacgcg
     1561 cgacaatgca tatcctgcct gagcccgtaa ccaaaaagaa gccgaatgtt tggggaaggc
     1621 tttttctagc ttcaccgcga cttcaggact aattgcgcgt ttggcgttta caatttcaga
     1681 taaagcagaa cgcggcatat ccagcttttc cgcggtttcc gtaatgttga gccccaatgg
     1741 ttccaaatac atgactttca gcattaaacc ggggtgcggt ggatttttca tttttgccat
     1801 aactctttct ccttagtggt agtccacata atcaacaatg taggcattgc catctttaaa
     1861 ctcaaaaatg acgcgccaat tgccgttgac tttcaagctc caatgcgttt ttagtttgcc
     1921 ctttaaccgg tgcaattcag cgccttcaat gtccaattct tgaatggttt ccgcaatatc
     1981 cattgcgtac agcaacacgc gcaatttttc cgcatgatgc ggttgaattc ctcgagtgtc
     2041 atcatcttca aaaaaccgtt tcaatccttt gtgcgcaaaa gatttaatca tcgatgtacc
     2101 ttgaaagaaa ggcggtattg tatcgcgtaa cgtgacgggg ttcaaagaca atgccacaag
     2161 taagcgcggc atcagaaaat taagtcatga ggaaaaacgg taaccaccac ggtaaccact
     2221 atggtaacca ctgcctaaaa catgcttatc ttttaaaaga tc
//