From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: D.pseudoobscura Adh gene for alcohol dehydrogenase
Date: 28 Dec 92 21:27:22 GMT
Distribution: bionet
Checksum: 23922 8

LOCUS       DROADHGN47   3444 bp ds-DNA             INV       01-DEC-1992
DEFINITION  D.pseudoobscura Adh gene for alcohol dehydrogenase
ACCESSION   X62227
KEYWORDS    ADH gene; alcohol dehydrogenase.
SOURCE      Drosophila pseudoobscura DNA.
  ORGANISM  Drosophila pseudoobscura
            Eukaryota; Animalia; Metazoa; Arthropoda; Uniramia; Insecta;
            Pterygota; Neoptera; Holometabola; Diptera; Brachycera;
            Cyclorrhapha; Schizophora; Drosophiloidea; Drosophilidae.
REFERENCE   1  (bases 1 to 3444)
  AUTHORS   Schaeffer,S.W. and Miller,E.L.
  TITLE     Estimates of gene flow in Drosophila pseudoobscura determined from
            nucleotide sequence analysis of the alcohol dehydrogenase region
  JOURNAL   Genetics 132, 471-480 (1992)
  STANDARD  full automatic
COMMENT     *source: strain=MXZ26; *source: isolate=Zirahuen, Mexico; *source:
            chromosome=IV section 88;
               From EMBL    entry DPADHGN47;  dated 16-OCT-1992.
FEATURES             Location/Qualifiers
     mRNA            join(40..119,891..996,1060..1464,1525..1940)
                     /note="adult mRNA (Adh)"
     mRNA            join(858..996,1060..1464,1525..1940)
                     /note="larval mRNA (Adh)"
     promoter        8..14
                     /note="adult promoter (Adh)"
     prim_transcript 40..1940
                     /note="adult primary transcript (Adh)"
     exon            40..119
                     /note="adult exon 1a"
     intron          120..890
                     /note="adult intron"
     promoter        822..829
                     /note="larval promoter (Adh)"
     prim_transcript 858..1940
                     /note="larval primary transcript (Adh)"
     exon            858..996
                     /note="larval exon 1"
     exon            891..996
                     /note="adult exon 1b"
     intron          997..1059
                     /number=1
     exon            1060..1464
                     /number=2
     intron          1465..1524
                     /number=2
     exon            1525..1940
                     /number=3
     polyA_signal    1917..1922
     polyA_site      1940
     CDS             join(904..996,1060..1464,1525..1791)
                     /EC_number="1.1.1.1"
                     /gene="Adh"
                     /product="alcoholdehydrogenase"
                     /codon_start=1
                     /translation="MSLTNKNVVFVAGLGGIGLDTSRELVKRNLKNLVILDRIDNPAA
                     IAELKAINPKVTITFYPYDVTVPVAETTKLLKTIFAQVKTIDVLINGAGILDDHQIER
                     TIAVNYTGLVNTTTAILDFWDKRKGGPGGIICNIGSVTGFNAIYQVPVYSGSKAAVVN
                     FTSSLAKLAPITGVTAYTVNPGITKTTLVHKFNSWLDVEPRVAEKLLEHPTQTSQQCA
                     ENFVKAIELNKNGAIWKLDLGTLEPITWTQHWDSGI"
BASE COUNT      979 a    783 c    784 g    898 t
ORIGIN      
        1 cgtagtgtat ttaaggcact gcacatgtcg aggaccacga ttattgtcta cagtgcagtc
       61 agtagttgtc aggtgcagca ggcggaccag catttcttta aatctactca atcgatcaag
      121 taagtaactg taatccaagt acagtcggcc catcccactc ggttccgcag tcactcaaga
      181 aatttcttga tagcgcagtt ccagttgtaa aacgtcgttt aaaaaaacat ctcgttggag
      241 cttgggttgg aaaacgttcg agttgggcgt agaaaatttc agatataaaa tatcaaaaaa
      301 gagaaaatat cttcccaaag cagctgtcca ttttttgtta aagtgataga tcaaaaattc
      361 gtgtgacgta cattcctagt gcacttcata caaacaattt cctcaattct aattctacaa
      421 gaagcacttt tggctcttgt ccagaaaaaa caatttgttt acacttgtcg gatgataaca
      481 gaagcggacc gacggctaag taacataaca gtcgcctgtt agatggccgc gcgtgtcgac
      541 tccgatagac cagcagacac gcagtagttt tcctcaagaa cctgaactct aaacatagac
      601 ataatttacg ataccttgag actcaaataa aaaatacaag tatgagtggt attctattcc
      661 tgattagact cgcacacaca catacgagta ttaacataaa tttgccggca aataaccaaa
      721 acaaaacgaa cgcaaacgat ccgaacacgt aatgcgagag ataagaaaca gaaaagctcg
      781 acgtgaaaga agcttctggc ggaagctctt gccgaactcc ctataaatac gagacagaaa
      841 accagaataa atataataac aaactgttga attgtcccat cgattcccag atcaaaagac
      901 agaatgtctc tcaccaacaa gaacgtcgtt ttcgtggccg gtctgggcgg cattggcctg
      961 gacaccagcc gggagttggt caagcgtaat ctgaaggtaa gagagaacga attccatgga
     1021 atctatggaa tcctaaattt aaaaattaat ttattttaga acctggtcat cctggatcgc
     1081 attgacaatc cggctgccat tgccgagctg aaggcaatca atcccaaggt gaccatcacc
     1141 ttctatccct acgatgtgac tgtgcccgtc gctgagacca ccaaactcct gaagaccatc
     1201 tttgcccagg tgaagacaat cgatgtcctg atcaacggtg ctggcatcct ggacgatcat
     1261 cagatcgagc gcaccattgc cgttaactac acgggcctgg tcaacaccac cacagccatt
     1321 ctggacttct gggacaagcg caagggcggc ccaggcggca tcatttgcaa cattggatcc
     1381 gtcaccggtt tcaatgccat ctaccaggtg cccgtttact ccggctccaa ggcggcggtg
     1441 gttaacttta cctcctccct ggcggtaaga aatcctaaaa ctatcctact ccccaaaact
     1501 aaatatttct ttgaaatccc atagaaactg gctcccatta ctggtgtcac tgcttacact
     1561 gtcaatcctg gcatcactaa gaccactctg gtccacaaat tcaactcgtg gctggatgtg
     1621 gagccccgtg tggcagagaa gctgctcgag catcccaccc agacctctca gcagtgcgcc
     1681 gagaactttg tgaaggccat tgagctgaac aagaacggtg ccatctggaa gttggatctg
     1741 ggcaccttgg agcccatcac atggacccag cactgggact cgggcatcta aaccatccca
     1801 gagactctat gggacatggc gttttagctt ttagtttctt tttttccact caattgttac
     1861 gtatatacat acacatatgg caataaggct gatttgaacc ccgttttgaa tatgataata
     1921 caaaattata tttgagaaat ttcaacaaaa tcgataagag ctatagctct cttttaatta
     1981 aaaaaaacta gataaaaaga gcaatggtca gtggtggtca gtggtcgttg tggcctctat
     2041 cgatttccac acaaaaactt tactgtttag taatagaaaa gaacttgaga aaggcagcca
     2101 aaagaatgta cgatctgacg ggtaagcatg tctgctatgt ggctgactgc ggtggcattg
     2161 cactggagac cagcaaggtt ctcatgacca agaatatagc ggtgagtggt agagaggtgg
     2221 tgtagagagt ggagtgtggt tctggttctt aaacaagtct ctagtctcta gtctctagtc
     2281 tcttggctgc tgctggtagc tgtcgaaact aattaagtta atgacttttt catgttttgt
     2341 ttttgtccgc tgacttttgc tgttcaaagc tacaattaag tttatgtttt gagtttgatc
     2401 tacttttgct taagggaagt actttcgatt acggattgct ggcatataaa aatagatacc
     2461 aaggaatgtg catcatatca tattgattct atcttgattc tttgactaga aactggccat
     2521 ccttcagagc gtggaaaacc ccccggccat cgctcagtta caatcgatta agcacagcac
     2581 acagatcttc ttctggacct tcgatgtgac catggcccgc gaggagatga agaagtactt
     2641 cgatgaggtc atggtccaga tggactacat cgatgtactg atcaatgggg ccaccctgtg
     2701 cgatgagcgg aacattgatg ccaccatcaa tacgaatctg accggcatga tgaacaccgt
     2761 ggccactgtg ctgccctata tggaccgaaa gatgggcggt tcgggtggac tgattgtgaa
     2821 tgtcacctcc gtcataggac tggatccatc gccagtcttt tgtgcgtaca gtgcctcaaa
     2881 gtttggtgtg attgggttca cccgaagtct agcggtgagt gggcgagatc ttaacttgaa
     2941 aatgctctct aatatcttcc attctttcgc aggatcccct gtattacacc caaaatggtg
     3001 tggctgtcat ggccgtctgc tgtggtccca ccaaagtgtt tgtcgatcgg gaactgactg
     3061 ccttcctgga atacggccaa acctttgccg atcgcctgcg tcgtgccccc tgccaatcga
     3121 cggccgtctg cggccagaat atagtaaatg ccatcgagag atcggagaac ggacagatct
     3181 ggattgccga caagggcggc ctggagtcgg tggccctcca ctggtattgg catatggccg
     3241 atcagttttt gaactacatg cagagcactg acgatgagga tcaggagttt tttttggggc
     3301 agcgatgagg catgccttga tccacggaat aaaacatcaa atgtgtcaca aaaagtaggc
     3361 aacttgtttg taaatagatt tgaaatattt tgaggtcata tgaataatga cataaatgag
     3421 ggtgcctgaa agtatgccaa gttt
//