From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: M.bovis ArgF gene for ornithine carbamoyltransferase
Date: 28 Dec 92 21:30:16 GMT
Distribution: bionet
Checksum: 44325 4

LOCUS       MSGARGF      1124 bp ds-DNA             BCT       01-DEC-1992
DEFINITION  M.bovis ArgF gene for ornithine carbamoyltransferase
ACCESSION   X64203
KEYWORDS    argF gene; ornithine carbamoyltransferase.
SOURCE      Mycobacterium bovis DNA.
  ORGANISM  Mycobacterium bovis
            Prokaryotae; Firmicutes; Mycobacteria; Mycobacteriaceae.
REFERENCE   1  (bases 1 to 1124)
  AUTHORS   Timm,J., Van Rompaey,I., Tricot,C., Massaer,M., Haeseleer,F.,
            Fauconnier,A., Stalon,V., Bollen,A. and Jacobs,P.
  TITLE     Molecular cloning, characterization and purification of ornithine
            carbamoyltransferase from Mycobacterium bovis-BCG
  JOURNAL   Mol. Gen. Genet. 234, 475-480 (1992)
  STANDARD  full automatic
COMMENT     *source: strain=BCG; *source: sub_strain=Pasteur GL2; *source:
            sub_clone=pNIV2202, pNIV2203;
               From EMBL    entry MBARGF;  dated 14-OCT-1992.
FEATURES             Location/Qualifiers
     CDS             184..1107
                     /EC_number="2.1.3.3"
                     /gene="ArgF"
                     /evidence="EXPERIMENTAL"
                     /note="subunit structure homotrimer"
                     /product="ornithine carbamoyltransferase"
                     /codon_start=1
                     /translation="MIRHFLRDDDLSPAEQAEVLELAAELKKDPVSRRPLQGPRGVAV
                     IFDKNSTRTRFSFELGIAQLGGHAVVVDSGSTQLGRDETLQDTAKVLSRYVDAIVWRT
                     FGQERLDAMASVATVPVINALSDEFHPCQVLADLQTIAERKGALRGLRLSYFGDGANN
                     MAHSLLLGGVTAGIHVTVAAPEGFLPDPSVRAAAERRAQDTGASVTVTADAHAAAAAA
                     DVLVTDTWTSMGQENDGLDRVKPFRPFQLNSRLLALADSDAIVLHCLPAHRGDEITDA
                     VMDGPASAVWDEAENRLHAQKAVLVWLLERS"
BASE COUNT      170 a    404 c    382 g    168 t
ORIGIN      
        1 catcgcgctg accgccccgc acgccaagga cgccgaggcc accgcccgcg acgccggtta
       61 cctggtcaac gcggccgcac ccgacgtcat ccggttggcg ccgccgctga tcatcgccga
      121 agcacagctc gacggctttg tcgcccgctt gccggcaatc ctggaccgcg ccgtgggggc
      181 cccgtgatca ggcatttcct gcgcgacgac gatctgtccc cggccgaaca ggccgaggtg
      241 ctcgagctcg cggccgagct gaagaaagac ccggttagcc gtcgtcccct gcaagggccg
      301 cgcggggtgg cggtcatctt cgacaagaac tccacccgca cccggttctc cttcgagctg
      361 ggcatcgcgc agctgggcgg gcatgccgtc gtcgtcgaca gcggcagcac ccagctgggc
      421 cgcgacgaaa ccctgcagga caccgcaaag gtgttgtccc gctacgtcga tgccatcgtc
      481 tggcgaacct tcggccaaga gcggctggac gccatggcgt cggtcgcgac ggtgcccgtg
      541 atcaacgcgc tctccgatga gttccatccg tgtcaggtgt tggccgacct gcagaccatc
      601 gccgaacgca agggggcgct gcgcggcctg aggttgtcct acttcggcga cggcgccaac
      661 aacatggccc actcgctgct gctcggcggg gtcaccgcgg gtatccacgt caccgtcgcg
      721 gctcccgagg gcttcctgcc cgacccgtcg gtgcgggccg cggccgagcg ccgcgcccag
      781 gataccggcg cctcggtgac tgtgaccgcc gacgcccacg cggccgccgc cgcggccgac
      841 gttctggtca ccgacacctg gacgtcgatg ggccaggaaa acgacgggtt ggaccgagtg
      901 aagccgtttc ggccgtttca gctcaactcg cgacttctgg cgctggccga ctcggatgcc
      961 atcgtgttgc attgcctgcc ggcccatcgc ggcgacgaga tcaccgacgc ggtgatggac
     1021 gggccggcca gcgcggtgtg ggacgaggcc gaaaaccggc tgcacgcgca gaaggccgtg
     1081 ctggtgtggc tgctggagcg ctcatgagcc gcgccaaggc cgcg
//