From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: A.rabiei cut gene for cutinase
Date: 29 Jul 92 10:01:11 GMT
Distribution: bionet
Checksum: 24809     4

LOCUS       AOCCUT       1662 bp ds-DNA             PLN       29-JUL-1992
DEFINITION  A.rabiei cut gene for cutinase
ACCESSION   X65628
KEYWORDS    cut gene; cutinase.
SOURCE      Ascochyta rabiei DNA.
  ORGANISM  Ascochyta rabiei
            Eukaryota; Fungi; Eumycota; Deuteromycotina; Coelomycetes;
            Sphaeropsidales; Sphaeropsidaceae.
REFERENCE   1  (bases 1 to 1662)
  AUTHORS   Tenhaken,R. and Barz,W.
  TITLE     Characterization and cloning of cutinase from ascochyta rabiei
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
COMMENT     *source: clone_library=genomic cosmid; *source: clone=pCutAr;
            *source: isolate=CBS 534.65;
            From EMBL    entry ARCUT;  dated 13-MAY-1992.
FEATURES             Location/Qualifiers
     exon            <513..701
                     /number=1
     CDS             join(513..701,761..1243)
                     /product="cutinase"
                     /gene="cut"
                     /codon_start=1
                     /translation="MKFFAFSMLIGEASPIVLALRRTTLEVRQLDPIIRSELEQGSSS
                     SCPKAILIFARGSTEIGNMGVSAGPAVASALEAYGADQIWVQGVGGPYTADLPSNFLP
                     GGTSQSAINEAVRLFNEANTKCPSTPIVAGGYSQGTAVMAGAIPKLDAVRARVVGTVL
                     FGYTQNQQNNKGIKDYPQEDLQVYCEVGDLVCDGTLIITVSHFLYLEEAAGPAPEFLK
                     SKIGA"
     intron          702..760
                     /number=1
     exon            761..>1243
                     /number=2
BASE COUNT      358 a    441 c    460 g    403 t
ORIGIN
        1 tcgacgacag caagtattgt tgatcatgca gtaagaataa ggtttcgtcg tctatcttag
       61 cataacgatg atcacaactt cgcatgaacg cactccaaac gatcatgcgc cggtccctcc
      121 cgtaccctga tccatgcacg gcattgcacg gtacttacca gctcccaagc gctgaaacgc
      181 ggcgcctgtc cacttcatcg tcattcctgg ctcgaggaga cgacgaaccg tagaagggta
      241 tgcttgtggt cgatgacgac ttcgtctaag ctctcttgga atgggggatt gaggcctgaa
      301 cctttgtttt cactaccact accgagggcg gggccaagtg gggcgggatg atggtgtccg
      361 tgatgttggg tctggaggtt gtagacattg gataagtata gcttgaggat tttccccgtt
      421 tactctatca tggtcaagag taacatcaaa gggaaattca gcccacactc gctctcttct
      481 taccttgttt tgtccatatc atcgcctgca tcatgaagtt tttcgctttc tccatgctta
      541 ttggcgaggc ctcgccaatc gtactggccc tgcggcggac aacgctggaa gtccgccagc
      601 tcgacccgat catcaggtca gaactcgagc agggcagcag ctcgagctgt ccaaaggcca
      661 tcttaatttt tgcccggggc agcaccgaga tcggcaatat ggtatgcccc tactcacctg
      721 caccccacca cacatgcacg tcttctaaca cttacaccag ggggtctccg ccgggccggc
      781 cgtggccagc gccctcgagg cctatggcgc cgaccaaatc tgggtccaag gcgtcggcgg
      841 cccctacact gcagatctcc cctccaactt cctgcccggc ggcacctcgc aatccgccat
      901 caacgaagcg gtgcgcctgt tcaacgaagc caacaccaaa tgcccctcga cccccatcgt
      961 ggccggcggc tacagccagg gaacggccgt catggccggc gcaatcccca aactcgacgc
     1021 cgtgcgcgcc agggtcgtgg gcacggtgct gtttgggtac acgcagaacc agcagaataa
     1081 caaggggatc aaagattacc cccaggagga tctgcaggtg tattgtgaag tgggcgatct
     1141 ggtttgtgac ggtacgctga tcatcactgt gtcgcacttt ttgtacttgg aggaggctgc
     1201 gggaccggcg ccggagtttc tgaagagcaa gattggggct tgagcttgag cttgagcttg
     1261 agcttgagct tgagcctgag cttggggtta gggttggggt tgtctgaact caagggctag
     1321 gagatgacgg tgggaggcag gagagttcgg ataggggcat gacggggttg gatgggggct
     1381 ttctcttctc ctctttagcg tcttcatttc tataatattt actgactcat gacatgttga
     1441 cgttggctgg ctagcctcaa acgccacttt ctacctgctc cgtgtactga gttgatgttc
     1501 tgaaagaatg gtaaagtgag aggttgagaa cgattctaca cagaccctaa gactcatgtg
     1561 ttttgatgaa tggaatttga ctgtcaatct ttgtagtcta cgatcgagat tgcaagctac
     1621 tagcttatcg tagtgagtcg cactcaagga ccatgttttg ta
//