From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human branched chain alpha-keto acid dehydrogenase (BCKDHB) E1-beta
Date: 20 Aug 92 10:02:19 GMT
Distribution: bionet
Checksum: 58504     5

LOCUS       HUMBCKDHA    1339 bp ss-mRNA            PRI       20-AUG-1992
DEFINITION  Human branched chain alpha-keto acid dehydrogenase (BCKDHB) E1-beta
            subunit mRNA, complete cds.
ACCESSION   M55575
KEYWORDS    branched chain alpha-keto acid dehydrogenase E1-beta subunit.
SOURCE      Human placenta, cDNA to mRNA, clone lambda-hBE-1-beta-1.
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 1339)
  AUTHORS   Matsuda,I., Asaka,J., Akaboshi,I., Endo,F., Mitsubuchi,H. and
            Nobukuni,Y.
  TITLE     Maple syrup urine disease: Complete primary structure of the
            E-1-beta subunit of human branched chain alpha-ketoacid
            dehydrogenase complex deduced from the nucleotide sequence and a
            gene analysis of patients with this disease
  JOURNAL   J. Clin. Invest. 86, 242-247 (1990)
  STANDARD  simple staff_review
FEATURES             Location/Qualifiers
     mRNA            <1..1339
                     /gene="BCKDHB"
     CDS             5..1183
                     /product="branched chain alpha-keto acid dehydrogenase
                     E1-beta subunit"
                     /gene="BCKDHB"
                     /codon_start=1
                     /translation="MAVVAAAAGWLLRLRAAGAEGHWRRLPGAGLARGFLHPAATVED
                     AAQRRQVAHFTFQPDPEPREYGQTQKMNLFQSVTSALDNSLAKDPTAVIFGEDVAFGG
                     VFRCTVGLRDKYGKDRVFNTPLCEQGIVGFGIGIAVTGATAIAEIQFADYIFPAFDQI
                     VNEAAKYRYRSGDLFNCGSLTIRSPWGCVGHGALYHSQSPEAFFAHCPGIKVVIPRSP
                     FQAKGLLLSCIEDKNPCIFFEPKILYRAAAEEVPIEPYNIPLSQAEVIQEGSDVTLVA
                     WGTQVHVIREVASMAKEKLGVSCEVIDLRTIIPWDVDTICKSVIKTGRLLISHEAPLT
                     GGFASEISSTVQEECFLNLEAPISRVCGYDTPFPHIFEPFYIPDKWKCYDALRKMINY
                     "
     sig_peptide     5..154
                     /gene="BCKDHB"
                     /codon_start=1
     mat_peptide     155..1180
                     /product="branched chain alpha-keto acid dehydrogenase
                     E1-beta subunit"
                     /gene="BCKDHB"
                     /codon_start=1
     polyA_signal    1327..1332
                     /gene="BCKDHB"
     polyA_site      1339
                     /gene="BCKDHB"
BASE COUNT      344 a    285 c    344 g    366 t
ORIGIN
        1 ggggatggcg gttgtagcgg cggctgccgg ctggctactc aggctcaggg cggcaggggc
       61 tgaggggcac tggcgtcggc ttcctggcgc ggggctggcg cggggctttt tgcaccccgc
      121 cgcgactgtc gaggatgcgg cccagaggcg gcaggtggct cattttactt tccagccaga
      181 tccggagccc cgggagtacg ggcaaactca gaaaatgaat cttttccagt ctgtaacaag
      241 tgccttggat aactcattgg ccaaagatcc tactgcagta atatttggtg aagatgttgc
      301 ctttggtgga gtctttagat gcactgttgg cttgcgagac aaatatggaa aagatagagt
      361 ttttaatacc ccattgtgtg aacaaggaat tgttggattt ggaatcggaa ttgcggtcac
      421 tggagctact gccattgcgg aaattcagtt tgcagattat attttccctg catttgatca
      481 gattgttaat gaagctgcca agtatcgcta tcgctctggg gatcttttta actgtggaag
      541 cctcactatc cggtcccctt ggggctgtgt tggtcatggg gctctctatc attctcagag
      601 tcctgaagca ttttttgccc attgcccagg aatcaaggtg gttataccca gaagcccttt
      661 ccaggccaaa ggacttcttt tgtcatgcat agaggataaa aatccttgta tattttttga
      721 acctaaaata ctttacaggg cagcagcgga agaagtccct atagaaccat acaacatccc
      781 actgtcccag gccgaagtca tacaggaagg gagtgatgtt actctagttg cctggggcac
      841 tcaggttcat gtgatccgag aggtagcttc catggcaaaa gaaaagcttg gagtgtcttg
      901 tgaagtcatt gatctgagga ctataatacc ttgggatgtg gacacaattt gtaagtctgt
      961 gatcaaaaca gggcgactgc taatcagtca cgaggctccc ttgacaggcg gctttgcatc
     1021 ggaaatcagc tctacagttc aggaggaatg tttcttgaac ctagaggctc ctatatcaag
     1081 agtatgtggt tatgacacac catttcctca catttttgaa ccattctaca tcccagacaa
     1141 atggaagtgt tatgatgccc ttcgaaaaat gatcaactat tgaccatata ggtaggtatg
     1201 catcttgaga aagctactat gtgcccctga cattaacgta ctgttaacca agacacagca
     1261 atcatcagtg ttttgatggt aacaaacttt gatggtaaag ttgataaaag gcaactttca
     1321 gaagaaaata atgtgcttt
//