From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Saccharomyces cerevisia (clone pYsup46) ribosomal protein S13
Date: 1 Sep 92 10:03:29 GMT
Distribution: bionet
Checksum: 55319     6

LOCUS       YSCSUP46A    1814 bp ds-DNA             PLN       01-SEP-1992
DEFINITION  Saccharomyces cerevisia (clone pYsup46) ribosomal protein S13
            (SUP46) gene, complete cds.
ACCESSION   M88650
KEYWORDS    40S ribosomal subunit protein S13.
SOURCE      Saccharomyces cerevisiae (strain GRF88) (library: YCp50 library of
            M. Rose) DNA.
  ORGANISM  Saccharomyces cerevisiae
            Eukaryota; Fungi; Eumycota; Hemiascomycetes; Endomycetales;
            Saccharomycetaceae.
REFERENCE   1  (sites)
  AUTHORS   All-Robyn,J.A., Kelley-Geraghty,D.K., Griffin,E., Brown,N. and
            Liebman,S.W.
  TITLE     Isolation of omnipotent suppressors in an [eta+] yeast strain
  JOURNAL   Genetics 124, 505-514 (1990)
  STANDARD  full staff_review
REFERENCE   2  (bases 1 to 1814)
  AUTHORS   Vincent,A. and Liebman,S.W.
  TITLE     The yeast omnipotent suppressor SUP46 encodes a ribosomal protein
            which is a functional and structural homolog of the Escherichia
            coli S4 ram protein
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     CDS             join(646..652,1066..1646)
                     /function="40S small subunit ribosomal protein"
                     /product="ribosomal protein S13"
                     /gene="SUP46"
                     /evidence=EXPERIMENTAL
                     /codon_start=1
                     /translation="MPRAPRTYSKTYSTPKRPYESSRLDAELKLAGEFGLKNKREIYR
                     ISFQLSKIRRAARDLLTRDEKDPKRLFEGNALIRRLVRVGVLSEDKKKLDYVLALKVE
                     DFLERRLQTQVYKLGLAKSVHHARVLITQRHIAVGKQIVNIPSFMVRLDSEKHIDFAP
                     TSPFGGARPGRVARRNAARKAEASGEAAEEAEDEE"
     enhancer        425..436
                     /standard_name="RAP1/GRF1 UAS"
                     /gene="SUP46"
                     /note="putative"
                     /citation=[2]
     enhancer        439..452
                     /standard_name="RAP1/GRF1 UAS"
                     /gene="SUP46"
                     /note="putative"
                     /citation=[2]
     exon            646..652
                     /number=X
                     /gene="SUP46"
     intron          653..1065
                     /number=1
                     /gene="SUP46"
     exon            1066..1646
                     /number=X+1
                     /gene="SUP46"
     allele          replace(1177,"a")
                     /phenotype="wild-type"
                     /note="changes Arg to Lys"
                     /gene="SUP46"
     allele          replace(1338,"a")
                     /phenotype="dominant omnipotent suppressor"
                     /standard_name="mutant 1"
                     /note="changes Asp to Asn"
                     /gene="sup46-1"
                     /citation=[1]
     allele          replace(1348,"g")
                     /phenotype="dominant omnipotent suppressor"
                     /standard_name="mutant 2"
                     /note="changes Leu to Trp"
                     /gene="sup46-2"
                     /citation=[1]
BASE COUNT      555 a    338 c    351 g    570 t
ORIGIN
        1 ataatatatg gctgaccact ctctttttgt ttcgtcttct gaatagtttc ttgtacaaaa
       61 ctattcgttg cgttagagct ctgtcctgtt tgaatcaccg tgtcatatac aggttcattt
      121 gaaatattta aaccaacaaa atcattggct tcttctttgc ctgttttgcc cagctggttg
      181 atacgggcac gtaactgatt taattcttgg tccctttcta gtgataaatc tcgcagagag
      241 ggcatactgg ttgtaaaaag tagtcgcaac aagtaagtcg taaaaaagta gatgtaatgg
      301 aaggctttga aggaacggct agccagcttt tctgtatcat tcctagccta attgacagca
      361 tcttgacctt ctagtatgga aactttcaat ttcagaaagc ggtgttcccc gctgcgacga
      421 tttaatccgt acatttacac atctgtacat ttttcatatt cgcaaaacaa aggttacttg
      481 aaaaaatcat aaagttggcg gcttcaggtg gacgcgcttc actcatgtag cttaacattc
      541 aatcccatta agcattatgc ataaatttca tgaagtttac ttaataaaat tgttcatttg
      601 catagacaag aaagaaagca acaagacaac taagactaag caacaatgcc aagtacgtat
      661 tagactatat cgaagaggag ggaaattctt cactctgact cgttgatttt gaaagaggtt
      721 cgggtcctca taaatatttg agaatatgaa attccataat agtatacctt cattgagtag
      781 cacgacaaca gcctgaatta ctatccatat tatgaatatc tttatttaca ctgaactccc
      841 gacacttcag ttaaacaggg atacattaga gatcaaggtg atctaatagg gaacatctct
      901 ctcgtaacaa tgggacagta ttttattttc caaggcggat acctaattat gcgtttttaa
      961 tcatatctct acaatattta tgagcactta cttgggccct tgcagacttt tgttccggga
     1021 aaacttttga ctaacaagaa tccaatttta cttttttttt tttaggagct ccaagaactt
     1081 actctaagac ttactctaca ccaaagagac cttacgaatc ttctcgtttg gacgcagaat
     1141 tgaagttggc cggtgaattc ggtttgaaga acaagagaga aatttacaga atttctttcc
     1201 aattgtctaa aattcgtcgt gctgccagag acttgttaac tagagacgaa aaggacccaa
     1261 agagattgtt cgaaggtaat gccttgatca gaagattggt tagagttggt gtcttgtccg
     1321 aagacaagaa gaagttggat tatgttttgg ctttgaaggt tgaagatttc ttggaaagaa
     1381 gattgcaaac tcaagtctac aagttgggtt tggccaagtc tgtccaccac gccagagttt
     1441 taatcactca aagacacatt gctgttggta agcaaatcgt caacatccca tctttcatgg
     1501 tcagattgga ctctgaaaag cacattgact tcgctccaac ttctccattc ggtggtgcta
     1561 gaccaggtag agttgctaga agaaacgctg ctagaaaggc tgaagcttcc ggtgaagctg
     1621 ctgaagaagc cgaagacgaa gaataaataa ttcgctgatt tcataactgt ataataaatt
     1681 ttataacttt tcaaatcacg gatcattttt cattttactt tgtcattttt atttcatttg
     1741 aaaatgggta gaggtacata actggcgctt ttcttgcaag atgacatggc attggatgac
     1801 acaagtatta ctca
//