From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: D.pseudoobscura Adh gene for alcohol dehydrogenase
Date: 28 Dec 92 21:28:06 GMT
Distribution: bionet
Checksum: 12031 8

LOCUS       DROADHGN77   3475 bp ds-DNA             INV       01-DEC-1992
DEFINITION  D.pseudoobscura Adh gene for alcohol dehydrogenase
ACCESSION   X64486
KEYWORDS    ADH gene; alcohol dehydrogenase.
SOURCE      Drosophila pseudoobscura DNA.
  ORGANISM  Drosophila pseudoobscura
            Eukaryota; Animalia; Metazoa; Arthropoda; Uniramia; Insecta;
            Pterygota; Neoptera; Holometabola; Diptera; Brachycera;
            Cyclorrhapha; Schizophora; Drosophiloidea; Drosophilidae.
REFERENCE   1  (bases 1 to 3475)
  AUTHORS   Schaeffer,S.W. and Miller,E.L.
  TITLE     Estimates of gene flow in Drosophila pseudoobscura determined from
            nucleotide sequence analysis of the alcohol dehydrogenase region
  JOURNAL   Genetics 132, 471-480 (1992)
  STANDARD  full automatic
COMMENT     *source: strain=PS283; *source: isolate=Kiabab National Forest,
            Arizona; *source: chromosome=IV section 88;
               From EMBL    entry DPADHGN77;  dated 16-OCT-1992.
FEATURES             Location/Qualifiers
     mRNA            join(40..119,914..1019,1083..1487,1548..1963)
                     /note="adult mRNA (Adh)"
     mRNA            join(881..1019,1083..1487,1548..1963)
                     /note="larval mRNA (Adh)"
     promoter        8..14
                     /note="adult promoter (Adh)"
     prim_transcript 40..1963
                     /note="adult primary transcript (Adh)"
     exon            40..119
                     /note="adult exon 1a"
     intron          120..913
                     /note="adult intron"
     prim_transcript 881..1963
                     /note="larval primary transcript (Adh)"
     exon            881..1019
                     /note="larval exon 1"
     exon            914..1019
                     /note="adult exon 1b"
     intron          1020..1082
                     /number=1
     exon            1083..1487
                     /number=2
     intron          1488..1547
                     /number=2
     exon            1548..1963
                     /number=3
     polyA_signal    1940..1945
     polyA_site      1963
     CDS             join(927..1019,1083..1487,1548..1814)
                     /EC_number="1.1.1.1"
                     /gene="Adh"
                     /product="alcoholdehydrogenase"
                     /codon_start=1
                     /translation="MSLTNKNVVFVAGLGGIGLDTSRELVKRNLKNLVILDRIDNPAA
                     IAELKAINPKVTITFYPYDVTVPVAETTKLLKTIFAQVKTIDVLINGAGILDDHQIER
                     TIAVNYTGLVNTTTAILDFWDKRKGGPGGIICNIGSVTGFNAIYQVPVYSGSKAAVVN
                     FTSSLAKLAPITGVTAYTVNPGITKTTLVHKFNSWLDVEPRVAEKLLEHPTQTSQQCA
                     ENFVKAIELNKNGAIWKLDLGTLEPITWTQHWDSGI"
BASE COUNT      966 a    793 c    803 g    913 t
ORIGIN      
        1 cgtagtgtat ttaaggcact gcacatgtcg aggaccacga ttattgtcta cagtgcagtc
       61 agtagttgtc aggtgcagca ggcggaccag catttcttta aatctactca atcgatcaag
      121 taagtaactg taatccaagt acagtcggcc catcccactc ggttctgccg tagctcaaga
      181 aatttcttga tagcgcagtt ccagttgtaa aacgtcgttt aaaaaaacat ctcgttggag
      241 cttgggttgg aaaacgttcg agttgggcgt agaaaagttc aaatataaaa tatcaaaaag
      301 agaaaatatc ttcccaaagc agctgtccat tttttgttaa agtgatagat caaaaattcg
      361 tatgacgtat ttcccaagtg ctctttatgc aaataatttc ctcaattcta attctacaag
      421 aagcactttt ggcacttgtc cagaaaaaac aatttgttta cacttttcgg ttgataacag
      481 aagcggaccg acggctaagt aacataacag tcgcctgtta agtaatgccc tcactcgctg
      541 ttagatggcc gcgcgtgtcg actccgatag accagcagac acgcagtagt tttcctcaag
      601 aacctgaact ctaaacatag acataattta cgataccttg agactcaaat aaaaatacaa
      661 gtatgagtgg tattctattc ctgattagac tcgcacacac acacgtacga gtattaacat
      721 aaatttgccg gcaaataacc aaaacaaaac gaacgcaaac gatccgaaca cgtaatgcga
      781 gagataagaa acagaaaagc tcgacgtgaa agaagcttct ggcggaagct cttgccgaac
      841 tccctataaa tacgagacag aaaaccagaa taaatataat aacaaactgt tgaattgtcc
      901 catcgattcc cagatcaaaa gacagaatgt ctctcaccaa caagaacgtc gttttcgtgg
      961 ccggtctggg cggcattggc ctggacacca gccgggagtt ggtcaagcgt aatctgaagg
     1021 taagagtgaa cgaattccat ggagtctatg gaatcctaaa tttaaaaatt tatttttttt
     1081 agaacctggt catcctggat cgcattgaca atccggctgc cattgccgaa ctgaaggcaa
     1141 tcaatcccaa ggtgaccatc accttctatc cctacgatgt gactgtgccc gtcgctgaga
     1201 ccaccaagct cctgaagacc atctttgccc aggtgaagac aatcgatgtc ctgatcaacg
     1261 gtgctggcat cctggacgat catcagatcg agcgcaccat tgccgttaac tacacgggcc
     1321 tggtcaacac caccacagcc attctggact tctgggacaa gcgcaagggc ggcccaggcg
     1381 gcatcatttg caacattggc tccgtcaccg gtttcaatgc catctaccag gtgcccgttt
     1441 actccggctc caaggcggcg gtggttaact ttacctcctc cctggcggta agtaatccaa
     1501 cgtttttcct actccccaaa actaaatatt tctttgaaat cccatagaaa ctggctccca
     1561 ttactggtgt cactgcttac actgtcaatc ctggcatcac taagaccact ctggtccaca
     1621 aattcaactc gtggctggat gtggagcccc gtgtggcaga gaagctgctc gagcatccca
     1681 cccagacctc tcagcagtgc gccgagaact ttgtgaaggc catcgagctg aacaagaacg
     1741 gtgccatctg gaagttggat ttgggcacct tggagcccat cacatggacc cagcactggg
     1801 actcgggcat ctaaaccagc ccagagactc tatgggacat ggcgttttag cttttagttt
     1861 ctttttttcc actcaattgt tacgtatata catacacata tggcaataag gctgatttga
     1921 accccgtttt gaatatgata atacaaaatt atatttgaga aatttcaaca aaatcgataa
     1981 gagctatagc tctcttttaa ttaaaaaaaa ctagataaaa agagcaatgg tcagtggtgg
     2041 tcagtggtcg ttgtggcctc tatcgatttc cacacaaaaa ctttactgtt tagtaataga
     2101 aaagaacttg agaaaggcag ccaaaagaat gtacgatctg acgggtaagc atgtctgcta
     2161 tgtggctgac tgcggtggca ttgcactgga gaccagcaag gttctcatga ccaagaatat
     2221 agcggtgagt ggtggagagg tggtgtagag agtggagagt ggttctggtt cttaaacaag
     2281 tctctagtct ctagtctcta gtctcttggc tgctgctggt agctgtcgaa actaattaag
     2341 ttaatgactt tttcatgttt tgtttttgtc cgctgacttt tgctgttcaa agctacaatt
     2401 aagtttatgt tttgagtttg atctactttt gcttaaggga agtactttcg attacggatt
     2461 gctggcatat aaaaatagat accaaggaat gtgcatcata tcatattgat tctatcttga
     2521 ttctttgccc agaaactggc catccttcag agcgtggaaa accccccggc catcgctcag
     2581 ttacaatcga ttaagcacag cacacagatc ttcttctgga ccttcgatgt gaccatggcc
     2641 cgcgaggaga tgaagaagta cttcgatgag gtcatggtcc agatggacta catcgatgtg
     2701 ctgatcaatg gggccaccct gtgcgatgag cggaacattg atgccaccat caatacgaat
     2761 ctgaccggca tgatgaacac cgtggccacg gtgctgccct atatggaccg taagatgggc
     2821 ggttcgggtg gactgattgt gaatgtcacc tccgtcatag gactggatcc atcgccagtc
     2881 ttttgtgcgt acagtgcctc aaagtttggt gtgattgggt tcacccgaag tctagcggtg
     2941 agtgggcgag atcctaactt gaaaatgctc tctaatgtct tccattcttc cgcaggatcc
     3001 cctgtattac acccaaaatg gtgtggctgt catggccgtc tgctgtggtc ccaccaaagt
     3061 gtttgttgat cgggaactga ctgccttcct ggcatacggc cagtcctttg ccgatcgcct
     3121 gcgccgtgcc ccctgccaat cgacggccgt ctgcggcaag aatatagtaa atgccatcga
     3181 gagatcggag aacggacaga tctggattgc cgacaagggc ggcctggagt cggtggccct
     3241 ccactggtat tggcatatgg ccgatcagtt tgtgaactat atgcagagca ctgacgatga
     3301 ggatcaggag ttttttttgg ggcagcgatg aggcatgcct tgatccacgg aataaaacat
     3361 caaatgtgtc acaaaaagta ggcaacttgt ttgtgggtct ggaaatagat ttgaaatatt
     3421 ttgaggtcat atgaataatg acataaatga gggtgcctga aagtatgcca agttt
//