From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Drosophila melanogaster alcohol dehydrogenase null4 allele,
Date: 18 Aug 92 10:00:36 GMT
Distribution: bionet
Checksum: 17279     6

LOCUS       DROADHN4     2369 bp ds-DNA             INV       18-AUG-1992
DEFINITION  Drosophila melanogaster alcohol dehydrogenase null4 allele,
            (processed pseudogene).
ACCESSION   X04454
KEYWORDS    alcohol dehydrogenase.
SOURCE      Drosophila melanogaster DNA.
  ORGANISM  Drosophila melanogaster
            Eukaryota; Animalia; Metazoa; Arthropoda; Uniramia; Insecta;
            Pterygota; Neoptera; Holometabola; Diptera; Brachycera;
            Cyclorrhapha; Schizophora; Drosophiloidea; Drosophilidae.
REFERENCE   1  (bases 1 to 2369)
  AUTHORS   Ashburner,M.
  JOURNAL   Unpublished (1986)
  STANDARD  full automatic
REFERENCE   2  (bases 1 to 2369)
  AUTHORS   Chia,W., Savakis,C., Karp,R. and Ashburner,M.
  TITLE     Adh:n4 of Drosophila melanogaster is a nonsense mutation
  JOURNAL   Nucleic Acids Res. 15, 3931-3931 (1987)
  STANDARD  full automatic
COMMENT     Adh:n4 is an EMS induced Adh-null allele changing gln82 to TAG. n4
            was induced on Adh:D, an EMS induced electrophoretic allele due, as
            shown here, to gly232->glu232. This sequence corresponds to bases
            4316-6671 of DMADHALL and all changes with respect to that sequence
            are annotated in that entry.
            From EMBL 26   entry DMADHN4;  dated 06-JUL-1989.
FEATURES             Location/Qualifiers
     CDS             join(1237..1335,1401..1805,1876..2142)
                     /pseudo
                     /gene="pseudo-ADH"
                     /codon_start=1
     TATA_signal     418..424
     exon            451..536
                     /number=1
                     /gene="pseudo-ADH"
     intron          537..1200
                     /number=1
                     /gene="pseudo-ADH"
     allele          897..936
                     /note="insertion 1a"
     TATA_signal     1135..1142
     misc_feature    1167..1236
                     /note="leader 2"
     exon            1201..1335
                     /number=2
                     /gene="pseudo-ADH"
     intron          1336..1400
                     /number=2
                     /gene="pseudo-ADH"
     exon            1401..1805
                     /gene="pseudo-ADH"
                     /note="putative"
                     /partial
     allele          1548
                     /note="n4 mutation (C->T)"
     intron          1806..1875
                     /number=3
                     /gene="pseudo-ADH"
     exon            1876..2320
                     /number=4
                     /gene="pseudo-ADH"
     allele          1949
                     /note="F mutation (A->C)"
     allele          2069
                     /note="D mutation (G->A)"
     3'UTR           2143..2320
     polyA_signal    2269..2274
                     /gene="pseudo-ADH"
BASE COUNT      726 a    544 c    475 g    618 t      6 others
ORIGIN
        1 ccctacactg taagaaatta ctatagaaat aacggtacac ggaataagat atttttttta
       61 gtccatatgc ttttaacaaa tgtgttttaa gtttatgtta tattattgtt agaaaaccgg
      121 tgttttttta aaatcggtta aaaaattact acgagagaaa aatacaaatt ttgtaaataa
      181 gattgactct ttttcgattt tggaatattt tcattcattt tatgttttta cgttttcact
      241 tatttgtttc tcagtgcact ttctggtgtt ccattttcta ttgggctctt taccccgcat
      301 ttgtttgcag atcacttgtt gcgcattttt attgcatttt acatattaca cattatttga
      361 acgccgctgc tgctgcatcc gtcgacgtcg actgcactcg cccccacgag agaacagtat
      421 ttaaggagct gcgaaggtcc aagtcatgca ttattgtctc agtgcagttg tcagttgcag
      481 ttcagcagac gggctaacga gtacttgcat ctcttcaaat ttacttaatt gatcaagtaa
      541 gtagcaaaag ggcacacaat tgaaggaaat tcttgtttaa ttgaatttat tatgcaagtg
      601 cggaaataaa atgacagtat taaatagtaa atattttgta aaatcatata taatcaaatt
      661 tattcaatca gaactaattc aagctgtcac aagtagtgcg aactcaatta attggcatcg
      721 aattaaaatt tggannnnnn ggccgcatat tcctcttgga aaatcacctg ttagttaact
      781 tctaaaaata ggaattttaa gataactcgt ccctgttaat cggcgccgtg ccttcgttag
      841 ctatctcaaa agcgagcgcg tgcagacgag cagtaatttt ccaagcatca ggcatataat
      901 atactaatac taatactaat actaatacta atataagaat actaatatag aaaaagcttt
      961 gccggtacaa aatcccaaac aaaaacaaac cgtgtgtgcc gaaaaataaa aataaaccat
     1021 aaactaggca gcgctccgtc gccggctgag cagcctgcgt acatagccga gatcgcgtaa
     1081 cggtagataa tgaaaagctc tacgtaaccg aagcttctgc tgtacggatc ttcctataaa
     1141 tacggggccg acacgaactg gaaaccaaca actaacggag ccctcttcca attgaaacag
     1201 atcgaaagag cctgctaaag caaaaaagaa gtcaccatgt cgtttacttt gaccaacaag
     1261 aacgtgattt tcgttgccgg tctgggaggc attggtctgg acaccagcaa ggagctgctc
     1321 aagcgcgatc tgaaggtaac tatgcgatgc ccacaggctc catgcagcga tggaggttaa
     1381 tctcgtgtat tcaatcctag aacctggtga tcctcgaccg cattgagaac ccggctgcca
     1441 ttgccgagct gaaggcaatc aatccaaagg tgaccgtcac cttctacccc tatgatgtga
     1501 ccgtgcccat tgccgagacc accaagctgc tgaagaccat cttcgcctag ctgaagaccg
     1561 tcgatgtcct gatcaacgga gctggtatcc tggacgatca ccagatcgag cgcaccattg
     1621 ccgtcaacta cactggcctg gtcaacacca cgacggccat tctggacttc tgggacaagc
     1681 gcaagggcgg tcccggtggt atcatctgca acattggatc cgtcactgga ttcaatgcca
     1741 tctaccaggt gcccgtctac tccggcacca aggccgccgt ggtcaacttc accagctccc
     1801 tggcggtaag ttgatcaaag gaaacgcaaa gttttcaaga aaaaacaaaa ctaatttgat
     1861 ttataacacc tttagaaact ggcccccatt accggcgtga cggcttacac tgtgaacccc
     1921 ggcatcaccc gcaccaccct ggtgcacacg ttcaactcct ggttggatgt tgagcctcag
     1981 gttgccgaga agctcctggc tcatcccacc cagccctcgt tggcctgcgc cgagaacttc
     2041 gtcaaggcta tcgagctgaa ccagaacgaa gccatctgga aactggactt gggcaccctg
     2101 gaggccatcc agtggaccaa gcactgggac tccggcatct aagaagtgat actcccaaaa
     2161 aaaaaaaaaa aacataacat tagttcatag ggttctgcga acagaagata ttcacgcaag
     2221 gcaataaggc tgattcgatg cacactcaca ttcttctcct aatacgataa taaaactttc
     2281 catgaaaaat atggaaaaat atatgaaaat tgagaaatcc aaaaaactga taaacgctct
     2341 acttaattaa aatagataaa tgggagcgg
//