From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Bacillus ssp. gene for subtilisin 1
Date: 21 Jul 92 10:03:36 GMT
Distribution: bionet
Checksum: 13262     4

LOCUS       BACSUBTIL1   1508 bp ds-DNA             BCT       21-JUL-1992
DEFINITION  Bacillus ssp. gene for subtilisin 1
ACCESSION   X62369
KEYWORDS    serine proteinase; subtilisin1 gene.
SOURCE      Bacillus sp. DNA.
  ORGANISM  Bacillus sp.
            Prokaryotae; Firmicutes; Endospore-forming rods and cocci;
            Bacillaceae.
REFERENCE   1  (bases 1 to 1508)
  AUTHORS   Narinx,E., Davail,S., Feller,G. and Gerday,C.
  TITLE     Nucleotide and derived amino acid sequence of the subtilisin from
            the antarctic psychrotroph Bacillus TA39
  JOURNAL   Biochim. Biophys. Acta 1131, 111-113 (1992)
  STANDARD  full automatic
COMMENT     *source: strain=TA39 (antarctic strain); *source: cell_line=
            Eschericia coli RR1; *source: clone=pSUBT1;
            From EMBL    entry BSSUBTIL1;  dated 11-JUN-1992.
FEATURES             Location/Qualifiers
     -35_signal      142..146
     -10_signal      161..166
     RBS             172..178
     CDS             185..1447
                     /EC_number="3.4.21.14"
                     /note="subtilisin 1"
                     /product="microbial serineproteinases"
                     /codon_start=1
                     /translation="MKRSGKIFTTAMLAVTLMMPAMGVSANEGNAAAEGNEKFRVLVD
                     SVDQKNLKNAKQQYGVHWDFAGEGFTTDMNEKQFNALKKNKNLTVEKVPELEIATATD
                     KPEALYNAMAASQSTPWGIKAIYNNSSITQTSGGGGINIAVLDTGVNTNHPDLRNNVE
                     QCKDFTVGTTYTNNSCTDRQGHGTHVAGSALADGGTGNGVYGVAPDADLWAYKVLGDD
                     GSGYADDIAAAIRHAGDQATALNTKVVINMSLGSSGESSLITNAVNYSYNKGVLIIAA
                     AGNSGPYQGSIGYPGALVNAVAVAALENKVENGTYRVADFSSRGYSWTDGDYAIQKGD
                     VEISAPGAAIYSTWFDGGYATISGTSMASPHAAGLAAKIWAQYPSASNVDVRGELQYR
                     AYENDILSGYYAGYGDDFASGFGFATVQ"
     stem_loop       1459..1490
BASE COUNT      435 a    344 c    390 g    339 t
ORIGIN
        1 gtcgactaaa tactcataaa attatcccga atgaagaatt cagacaaaat tcgacaatat
       61 ttttcccaat tacgctatat ctattcttct gctaacgtta gatgtgtggc aggggcttat
      121 gcgggaccgc gcacaaccgg attcagcgcc ataaaaaacc tatacgggat aaggagattg
      181 atttatgaag agatcaggaa agattttcac aaccgcaatg ctggctgtta ctttgatgat
      241 gcctgcaatg ggagtaagtg caaacgaagg aaacgcagca gccgaaggca atgaaaaatt
      301 ccgggtactg gttgactcag tcgatcagaa aaacctgaag aacgcgaaac agcagtacgg
      361 ggtacactgg gacttcgctg gtgaaggttt cactaccgat atgaatgaga agcaatttaa
      421 tgcattgaaa aaaaataaga atctgacggt ggagaaagtg ccggaattgg aaattgctac
      481 ggcaacggac aagccggaag ctttatataa tgcaatggcg gcatcccaat cgacaccatg
      541 gggcatcaaa gccatataca ataacagcag catcacccaa acgagcggcg ggggcggcat
      601 caatatcgca gtccttgata caggagtcaa cacaaaccac ccggatcttc gcaataatgt
      661 cgaacagtgc aaagatttca cagtcggaac cacttacacg aacaatagct gtacagaccg
      721 ccagggccac ggtacacacg ttgccggctc agcactcgct gatggtggaa ccggaaatgg
      781 cgtttacggt gtggcaccgg acgcagacct ctgggcgtat aaagtattgg gtgatgacgg
      841 atccggttac gcagatgaca tcgctgcagc tattcgccac gctggcgacc aggcaacggc
      901 acttaacacg aaagtggtca tcaatatgtc tctgggctct tcaggtgaaa gcagcctgat
      961 cacgaatgca gtgaactatt catataataa aggcgttctt atcattgccg cagccggaaa
     1021 ctccggtcca taccagggct cgatcggcta cccgggggca ttggtgaatg cagtggcagt
     1081 agcggcgctt gaaaacaaag tggaaaacgg cacttaccgt gttgcagatt tttcttcgcg
     1141 cggctacagc tggactgacg gcgattacgc catccaaaaa ggtgatgtcg aaatctcagc
     1201 gccaggtgcg gcaatctact ccacttggtt tgatgggggc tacgcgacaa tcagcggaac
     1261 ttcaatggct tcaccgcatg ctgccggttt agcagcgaaa atctgggcgc aatatccgtc
     1321 cgcttcaaat gttgatgtga gaggcgaatt gcaataccgc gcttacgaaa acgacatcct
     1381 atccggttac tatgctggat acggagatga ttttgcttca gggttcggat ttgcaacagt
     1441 tcaataagat catcagtttg ggcagttccg gtgtttcgcc ggagctgttt ttatatgggt
     1501 tatttaaa
//