From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Pea gTUB2 mRNA for beta-tubulin 2
Date: 15 Sep 92 10:05:02 GMT
Distribution: bionet
Checksum: 47869     4

LOCUS       PEAGTUB02    1511 bp ss-RNA             PLN       15-SEP-1992
DEFINITION  Pea gTUB2 mRNA for beta-tubulin 2
ACCESSION   X54845
KEYWORDS    beta-tubulin; tubulin.
SEGMENT     2 of 3
SOURCE      Pisum sativum RNA.
  ORGANISM  Pisum sativum
            Eukaryota; Plantae; Embryobionta; Magnoliophyta; Magnoliopsida;
            Rosidae; Fabales; Fabaceae.
REFERENCE   1  (bases 1 to 1511)
  AUTHORS   Cerff,R.
  JOURNAL   Unpublished (1990)
  STANDARD  full staff_entry
REFERENCE   2  (sites)
  AUTHORS   Liaud,M.F., Brinkmann,H. and Cerff,R.
  TITLE     The beta-tubulin gene family of pea: primary structures, genomic
            organization and intron dependant evolution of genes
  JOURNAL   Plant Mol. Biol. 18, 639-651 (1992)
  STANDARD  full staff_review
COMMENT     *source: strain=Rosakrone; *source: stage=seedlings, 10 days old;
            *source: tissue=green photosynthetic; *source: clone_library=EcoRI
            site of lambda NM1149; *source: clone=pPsTUB2;
            From EMBL    entry PSGTUB2;  dated 29-APR-1992.
FEATURES             Location/Qualifiers
     CDS             <1..1343
                     /partial
                     /note="codons 1-2 are missing"
                     /product="beta-tubulin 2"
                     /gene="gTUB2"
                     /codon_start=3
                     /translation="EIVHIQGGQCGNQIGAKFWEVVCAEHGIDPTGRYGGDTDLQLER
                     INVYYNEASCGRYVPRAVLMDLEPGTMDSVRSGPYGQIFRPDNFVFGQSGAGNNWAKG
                     HYTEGAELIDSVLDVVRKEAENCDCLQGFQVCHSLGGGTGSGMGTLLISKIREEYPDR
                     MMLTFSVFPSPKVSDTVVEPYNATLSVHQLVENADECMVLDNEALYDICFRTLKLTTP
                     SFGDLNHLISATMSGVTCCLRFPGQLNSDLRKLAVNLIPFPRLHFFMLGFAPLTSRGS
                     QQYRALSVPEITQQMWDSKNMMCAADPRHGRYLTASAIFRGKMSTKEVDEQMMNVQNK
                     NSSYFVEWIPNNVKSTVCDIPPTGLKMASTFIGNSTSIQEMFRRVSEQFTAMFRRKAF
                     LHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATAEEDEYEEEEEDYHQEHDEM"
     polyA_signal    1483..1488
BASE COUNT      401 a    291 c    366 g    453 t
ORIGIN
        1 gagaaattgt tcatatccaa ggtggccaat gcggaaacca gatcggagcc aaattctggg
       61 aagttgtttg cgccgaacat ggaatcgacc ctaccggaag gtatggtggt gacacagatc
      121 ttcaattaga gagaatcaac gtctattaca acgaagctag ctgtggtcgt tatgttcctc
      181 gagcggttct tatggatctt gaaccgggta ccatggacag tgtccggtct ggaccgtacg
      241 gtcagatttt tagaccggat aactttgtgt ttggtcagag tggtgctggg aataactggg
      301 ctaagggtca ttatactgag ggcgctgagc ttattgattc tgttctcgat gttgttcgga
      361 aagaagctga aaactgtgat tgcttgcagg gttttcaagt atgtcattca ctaggtggag
      421 gaactggatc tggaatgggt acccttttga tttcaaagat tagggaagaa taccctgata
      481 ggatgatgct tactttctcc gtttttcctt ctcctaaggt ctctgacaca gtggtcgaac
      541 cctacaatgc tactctctcg gttcaccagc ttgttgaaaa tgctgatgaa tgtatggttc
      601 ttgataatga agccttgtat gatatttgct tccgtaccct taagctcaca accccaagct
      661 ttggtgatct taaccatttg atatctgcta ccatgtctgg tgtcacatgt tgtctgaggt
      721 tccctggtca gcttaactct gatcttcgca agcttgctgt gaatctgatt ccgttccctc
      781 gtctccactt tttcatgttg gggtttgcac ctttgacttc tagaggctct cagcagtaca
      841 gggctctgag tgtacctgaa atcacacagc aaatgtggga ttccaagaac atgatgtgcg
      901 ctgccgatcc tagacatgga cgatacctca ctgcatccgc catcttcaga ggcaaaatga
      961 gcacaaagga agttgatgaa caaatgatga atgttcagaa caagaactca tcctactttg
     1021 tggaatggat tccaaacaat gtgaaatcaa ctgtttgtga tattccccca acaggtttga
     1081 agatggcttc aactttcatt ggaaactcaa cctcaattca ggaaatgttc agaagggtga
     1141 gtgagcaatt cactgctatg tttaggagga aggctttctt gcattggtac acaggtgagg
     1201 gaatggacga gatggaattc accgaagccg agagcaacat gaacgatctt gtatcagaat
     1261 accaacagta tcaagatgct actgctgagg aggatgagta tgaagaggaa gaggaagatt
     1321 atcatcagga acatgatgaa atgtgaggac tatgatatgg atagaatgat ctatttgcgg
     1381 tggaattttg gattgaatgt ggaacttatc atgccgcaac ttcctgttgc tggttcatta
     1441 tctatctgtt tgtaatgcta tatatacttc attccggttt atgtgaaatt aattgattat
     1501 gcttttattt c
//