From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: R.sphaeroides crtD gene for methoxyneurosporene dehydrogenase
Date: 21 Jul 92 10:08:18 GMT
Distribution: bionet
Checksum: 61852     5

LOCUS       RCACRTD      1794 bp ds-DNA             BCT       21-JUL-1992
DEFINITION  R.sphaeroides crtD gene for methoxyneurosporene dehydrogenase
ACCESSION   X63204
KEYWORDS    crtD gene; methoxyneurosporene dehydrogenase.
SOURCE      Rhodobacter sphaeroides DNA.
  ORGANISM  Rhodobacter sphaeroides
            Prokaryotae; Gracilicutes; Anoxyphotobacteria;
            Purple nonsulfur bacteria.
REFERENCE   1  (bases 1 to 1794)
  AUTHORS   Gari,E., Toledo,J.C., Gibert,I. and Barbe,J.
  TITLE     Nucleotide sequence of the methoxyneurosporene dehydrogenase gene
            from Rhodobacter sphaeroides: Comparison with other bacterial
            carotenoid dehydrogenases
  JOURNAL   FEMS Microbiol. Lett. 93, 103-108 (1992)
  STANDARD  full automatic
COMMENT     From EMBL    entry RSCRTD;  dated 11-JUN-1992.
FEATURES             Location/Qualifiers
     promoter        5..50
                     /note="E.coli-like sigma-70 promoter"
     misc_feature    40..63
                     /note="Palindromic motif"
     RBS             93..99
                     /gene="crtC"
     CDS             115..1602
                     /product="methoxyneurosporenedehydrogenase"
                     /gene="crtD"
                     /codon_start=1
                     /translation="MRQIVPKVVVVGAGMGGLASAIRLARAGCEVTLLEAREAPGGRM
                     RTLPSVAGPVDAGPTVLTLREVFDDIFEVCGQKLDHHLTLLPQPLLARHWWLDGSTLD
                     LTTDLEANVEAVAAFAGAREAPAFRRFHDLSARLYDAFDRPMMRAARPDLRAIATGAL
                     KAPRTWPALLPGMTLDRLLRLFFRDRRLRQLFGRYATYVGGTPYGAPGVLALIWAAEA
                     RGVWAIEGGMHRLALALARLADDQGVRLRYGAPVAGILRQGGRPTGVQLADGRTLPAD
                     HIVFNGDPAALLAGCLGDGPQDAVPEDRIHPRSLSAWVWSYAARASGPPLVHHNVFFA
                     DDPRREFGPIAAGQMPEDATLYICAEDRSGGQLPDGPERFEIIMNGPPGRPAKPEDFA
                     QCRSRTFDRLRQFGLTFDPVPGETSLTAPSGFASLFPASQGSIYGLSATGPLASLKRP
                     LARTALPGLWLAGGGAHPGAGVPMAALSGRHAAEAILADLASRSR"
     CDS             1663..>1794
                     /gene="crtC"
                     /codon_start=1
                     /translation="MIGFIGSVFSPWYRWSGRKEPQNHCCLNVATYGPGGRFTMTDRA
                     "
BASE COUNT      222 a    646 c    642 g    284 t
ORIGIN
        1 atcgttggcc cccgggcgcc accgtgccgc cgactccaca ggtgtcaact ttttcttaca
       61 ccttctggtc tttgatactg gacagcccgc acgggggtgt caacaatccc gggcatgaga
      121 cagattgtgc cgaaggtggt tgtggtcggg gccgggatgg gcgggctggc ctctgcgatc
      181 cggctggcgc gggccgggtg cgaggtgacg ctcctcgagg cgcgagaggc gccggggggg
      241 cggatgcgta ccctcccgag cgtggcggga ccggtggacg caggcccgac cgtgctcacg
      301 cttcgcgaag ttttcgacga tatcttcgag gtttgcggcc aaaagctcga tcatcacctc
      361 actctcctgc cccagccgct tctggcccgg cactggtggc tcgacggcag cacgctcgac
      421 ctcaccaccg acctcgaggc caatgtcgag gcggtggcgg ccttcgccgg cgcgcgcgag
      481 gccccggcct tccgccgctt ccacgatctc tcggcgcggc tttatgacgc tttcgaccgg
      541 ccgatgatgc gcgcggcccg gcccgacctg cgggccatcg cgaccggggc cctgaaggcg
      601 ccccggacat ggcccgcgct cctgcccggc atgacgctcg accggctcct gcggctcttc
      661 ttccgcgacc ggcggctgcg tcagctcttc gggcgctacg ccacctatgt gggcggcacg
      721 ccctacggtg cgccgggcgt gctcgcgctc atatgggcgg ccgaggcgcg cggggtctgg
      781 gcaatcgagg gcgggatgca ccggctcgcg ctggcgctgg cccgcctggc cgacgatcag
      841 ggcgtgcggc tgcgctacgg ggcgccggtc gccggcatcc tgcgccaggg cggccggccc
      901 accggcgtgc agctggccga cgggcggacg cttccggccg atcacatcgt cttcaacggc
      961 gatccggcgg cgctccttgc ggggtgtctg ggcgacgggc cgcaggacgc cgtgcccgag
     1021 gaccggatcc acccgcgcag cctctcggcc tgggtctggt cctatgccgc ccgcgcctcg
     1081 ggcccgccgc tcgtccatca caacgtcttc ttcgccgacg acccccggcg cgagttcgga
     1141 ccgatcgccg caggacagat gccggaggat gcgacactct acatctgcgc cgaggaccgc
     1201 tccggcgggc agctccccga cgggcccgag cgattcgaga tcatcatgaa cggaccgccc
     1261 ggccggcccg cgaaacccga ggattttgcg caatgtcgca gccgaacctt cgaccgcctg
     1321 agacagttcg ggctgacctt cgacccggtg ccgggcgaga cgagcctgac ggccccctcg
     1381 ggcttcgcga gcctgttccc ggcgtcgcag ggctcgatct acgggctgag cgccacgggg
     1441 ccgctggcct cgctgaagcg tccgctggcg cggacggcgc tgccgggcct gtggctggcg
     1501 gggggcgggg cccatccggg ggcgggcgtg ccgatggcgg ccctctccgg acggcacgcg
     1561 gccgaggcga tactggcgga ccttgcttct cggtcgaggt agcgccgacg ctatgcctgg
     1621 tggtatatcg acggcgtgtc cgacgacggc accgggctct cggtgatcgg cttcatcggc
     1681 tcggtcttct cgccctggta tcgctggtcg ggccggaagg agccgcagaa ccactgctgc
     1741 ctcaatgtgg cgacctacgg ccccggcggg cgcttcacca tgaccgaccg ggcc
//