From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: S.cerevisiae gene TSF3
Date: 10 Apr 92 10:15:05 GMT
Distribution: bionet
Checksum: 47552    10

LOCUS       YSCTSF3G     5674 bp ds-DNA             PLN       10-APR-1992
DEFINITION  S.cerevisiae gene TSF3
ACCESSION   X64516
KEYWORDS    transcriptional silencer.
SOURCE      Saccharomyces cerevisiae DNA.
  ORGANISM  Saccharomyces cerevisiae
            Eukaryota; Fungi; Eumycota; Hemiascomycetes; Endomycetales;
            Saccharomycetaceae.
REFERENCE   1  (bases 1 to 5674)
  AUTHORS   Chen,S., West,R.W., Johnson,S.L., Gans,H. and Ma,J.
  TITLE     Regulatory genes required for transcriptional silencing in yeast:
            cloning and characterization of TSF3 (SIN4)
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
COMMENT     *source: strain=YEp24; *source: clone=pSC-TSF3;
            From EMBL    entry SCTSF3G;  dated 20-MAR-1992.
FEATURES             Location/Qualifiers
     CDS             2043..4967
                     /note="TSF3 is identical to SIN4"
                     /product="unknown"
                     /gene="TSF3"
                     /translation="MMLGEHLMSWSKTGIIAYSDSQSSNANICLTFLESINGINWRFH
                     TPQKYVLHPQLHEVQYQESSSTLSTHSTTTSVNGSTTAGVGSTPNFGGNSNKSPPQFF
                     YNISSIHWNNWFSLPGDMLAVCDELGNMTMLITGQRPDRATTYEKLTMVFQDNVYKIY
                     NHVMPLKPVDKLKPMNIERKQTRKEYNTSILEFRWLTSSKSVIVSQFCAFDSSSNTYR
                     SRAQQVPPYGVYHPPFIKYACLAIRKNGQIDFWYQFSNSKDHKKITLQLLDTSNQRFK
                     DLQWLEFARITPMNDDQCMLITTYSKLSKNISFYKLHVNWNLNATKPNVLNDPSLKIQ
                     FILSTTLDPTDDEGHVLKLENLHVVSKSSIEKDPSPEILVLYNVCDTSKSLVKRYRLA
                     PTQLSAEYLVILKPDLNIDRNNSTNQIFQSRRYNLRRHSDIVLDKKVTLITSEMFDAF
                     VSFYFEDGTIESYNQNDWKLETERLISQSQLGKFKNIIASPLSAGFNYGKLPLPPSVE
                     WMKVSPSMCGVIVKQYNKKWPQFYAAVQKNYADPEKDSINATALAFGYVKSLHKQISA
                     EDLTIAAKTHILRISFLDRKRAKEFITTLLKSLYSFFNISPDAPKEIMDKIITSRPLQ
                     KIMLLQLELGSCFSQENIEEMARVILYLKNVLFAFNGVARNFHFAIEQISNNSNQQQN
                     PKLFQTIFSKQDLIHSLIPVAKWFVKFITYLTQEILILINDPTNKEYTLVHGIFGAKM
                     SRTLILSILNEIKKVTQIVAKFPETSYPILNESSTFLKLVLSESPVDFEKFETFLVDV
                     NNKFIALCEQQPSQEREFSLLVKAEIPPEYAKVGDFLLQYANNAVISHANAAAVYFAD
                     TSGLKISNSEFFNPEIFHLLQPLEEGLIIDTDKLPIKNRTSKSFSKLLYDDVTCDKLS
                     VSEISDGKLKRCSRCGSVTRAGNIISSDKTIVPTSIQTKRWPTMYTRLCICSGMLFEM
                     DG"
BASE COUNT     1819 a   1068 c   1093 g   1694 t
ORIGIN
        1 aagcttcaaa ttacaaaata gcattttttt cttatagatt ataatactct ctcatacgta
       61 tacgtatatg tgtatatgat atataaacaa acattaatat cctattcctt ccgtttgaaa
      121 tccctatgat gtactttgca ttgtttgcac ccgcgaataa aatgaaaact ccgaaccgat
      181 atatcaagca cataaaaggg gagggtccaa ttaatgcata tttaagacca cagctgaata
      241 actttaaaac ggcagacaaa acaaaaaata ggtcgaataa accttacctg cctagaagga
      301 atgacagcag ctaataagaa tattgtcttc ggattttcca gatccattag cgcaattcta
      361 ctaatatgct ttttctttga aaaagtctgc ggtgatatgg agcatgatat gggcatggat
      421 gatacttcgg gatacacgag gccagaaatt gtgcaggctg ggtcgaaatc tttccactgg
      481 ctctgcactt tgggattctt gttgctttta ccatccgtgg tgacgtgcct ttcgttcgct
      541 ggcaggatat attcagctac cctcttacaa tgcacttgtg ccgtttacgc tttcttagaa
      601 gctgccgtat taagatttca agacaatgat ggggtagaaa atagaacttc aaggggaacc
      661 gcatggtttt tggtgggact tacttggata accttattct ttggtggatt agctggagga
      721 actggtttct tagtgaaaag caagaggttg caaacgttca tatcaaatgc aggtgagaaa
      781 aggttgtcat atatccatcg tggtttatcc tttctaactg ttctaacagg ttgggttaaa
      841 gtctgtttgg cacctgttgc gctctttggg ttttgtagag aggcacacac agggcaatgc
      901 atcgctcatg gtatcatggg atccgcgttt gtgttgtacg ggttcattta tgtactggtt
      961 ttggttatac catggattcg cagtgcccaa acctcatatt cacaggatta cgtcgacagt
     1021 tgggttatgt gcatatgggg cgttgtaaat acttttactg aacacaggtg gggacgtgaa
     1081 ggatggagtg ttcacgatta tcaacacacg tttatgggaa ttatctggtg gactggtgga
     1141 atactcggga ttttcctttc cagaaatggc agaagaacat ttgtaccaag tttgattatt
     1201 attttcaccg gatgggccat gtctgagcac gcccagcact tgataattag cacaaaagtc
     1261 cataatatgt ttgggctagt tttaatgtgt ggaggcgctt taagaattat cgaaatatct
     1321 tttttactac gtgataaaag aacgctggac aaaatacatt cattccaata ccttgctccc
     1381 ttttgtttag tatgttcagg tctcctattt atgggcgcaa acgaagaaca actcatttta
     1441 gtgttgcggt tgggaggcga ccatagtgcg tacgttctaa ttatcgtatc aggcgcattt
     1501 ttggtctact tctggatgat tgcatgtttg gaattctacc tatatctact tgaaaaggga
     1561 aaacaaggat tccttccgaa atcatacgaa cttgaagagg aaaacaataa cgtgagcttt
     1621 gagctagata acatatcgaa cgaagatgtg gatgaagata ccactccctt taacgtttga
     1681 atagtgggca actaatgtaa tataagacat ttgaaagtcc gatcctacac cctctttgtt
     1741 agaagcattg taactttatt tttttttata atactctaaa tgcggaactg tatacacttt
     1801 gcacagtttg tattcaaatt gtttgcataa atatataaaa attgccacga acacttttag
     1861 attccttcgt tagtctcctt tgtatattgc cgtttgcagg ctcatgatga taaatctcgc
     1921 tatttcggtg aagaaaaaaa aagctagctt cgaacttttc aaaagaagta taatttcatt
     1981 tcaaaaataa ggtccaaaga aaagaaacta gcagacctga ccttctgttg gtaaatatta
     2041 gtatgatgct tggagagcat ttaatgagct ggtctaagac tggtattatt gcatacagtg
     2101 actcacagtc ctcaaatgcc aatatttgcc tcacattttt agaatccata aatggaataa
     2161 actggcgatt tcatacgccg caaaaatatg tattacaccc gcaattgcac gaagttcaat
     2221 accaagaaag tagtagtact ctaagcactc actcgacaac aacttctgtc aatggatcaa
     2281 ctacagccgg ggttggaagc actccaaact tcggaggcaa cagcaacaag tctcctccac
     2341 agttctttta taatatatct tccattcatt ggaacaactg gtttagttta ccaggtgaca
     2401 tgttggctgt atgtgacgaa ttaggtaaca tgaccatgtt aattactgga caacgtcctg
     2461 atcgggccac tacatacgaa aagttaacga tggtatttca agataatgtt tacaaaattt
     2521 ataaccacgt tatgccttta aagcctgtag acaagctaaa gcccatgaac attgaaagga
     2581 aacaaacaag gaaagaatac aatacatcca tcttggaatt tcgctggctc acctcttcaa
     2641 aatccgtaat cgtttctcaa ttctgcgcat ttgatagtag ttctaataca tatagaagca
     2701 gagcacagca agtgcctcca tatggtgtgt accatccacc ctttataaaa tatgcgtgct
     2761 tggcaataag gaaaaacggt caaatagatt tttggtacca attttcaaac tcaaaggatc
     2821 ataaaaaaat aacgttacag ctgctggaca cttcaaatca aagattcaag gatcttcaat
     2881 ggcttgaatt tgctagaatt acacctatga atgatgatca gtgtatgttg ataaccacat
     2941 attcaaaatt atctaaaaat atctcttttt acaaactaca cgttaattgg aaccttaatg
     3001 cgaccaaacc aaacgtccta aatgacccat cgttgaaaat acaatttata ttaagcacta
     3061 ctttagatcc aacggatgat gaagggcatg tgttgaagtt agagaacctg catgttgtgt
     3121 ctaaatcatc aattgagaaa gatccctccc cagaaatttt agttttgtat aatgtttgcg
     3181 atacatcaaa atcattggtg aaaagatata gactagctcc aacacaactt tcagctgagt
     3241 atttggtgat attgaaacca gatttaaata tcgatcgaaa taattctacg aatcaaatat
     3301 tccaatcgcg ccgttacaac cttcgtcgcc acagtgatat cgttctcgac aaaaaagtca
     3361 ccttgataac ttctgagatg ttcgatgcat ttgtttcatt ttactttgaa gatggtacca
     3421 tcgaatccta caatcaaaat gattggaagt tggagactga acgacttata agtcagagcc
     3481 aattaggaaa attcaaaaat attattgcat ccccactaag cgcaggattt aattatggga
     3541 agctcccttt acctccatcg gttgagtgga tgaaggtttc accatctatg tgtggggtta
     3601 tcgttaaaca gtataataaa aaatggccac agttttatgc tgctgttcaa aaaaactacg
     3661 cagatcctga gaaggattct ataaatgcta cagctttggc atttggatac gtgaaaagtt
     3721 tacataagca aatatctgcc gaagacttga ccatcgctgc gaagacgcac atactcagaa
     3781 tttcattctt ggatagaaaa agggccaaag aatttattac gactttgttg aaaagcctat
     3841 actcgttttt caacatttct cctgatgcgc ctaaagagat tatggataaa ataataacaa
     3901 gtaggccact tcaaaaaatt atgctattgc agctggaact tggcagttgt ttttcgcaag
     3961 aaaatattga agaaatggcg agagttatac tctacttgaa aaatgttttg tttgcgttca
     4021 atggtgtggc gagaaatttt cattttgcga ttgaacaaat aagtaataat tctaaccaac
     4081 aacaaaaccc gaagttgttc cagactatat tttcaaaaca ggatctcatt cattcattga
     4141 ttcctgttgc taaatggttt gtcaaattta tcacttattt gacccaagaa attttaattt
     4201 taataaatga tcccacaaac aaggaataca cactagttca cggaatattt ggagccaaaa
     4261 tgtccaggac tttgatattg tctattctta acgaaatcaa gaaagtcacc caaatagtgg
     4321 cgaaatttcc agaaacatca tacccaattt taaacgagtc atcaactttt ttgaaactag
     4381 tcttatctga atcaccagtg gatttcgaaa aatttgaaac gtttttagtt gatgtaaaca
     4441 acaaatttat cgcgctatgc gaacaacagc catcacagga acgtgagttt tcgttgttgg
     4501 tgaaagctga aatcccccca gaatatgcca aagtgggaga tttcctcctg cagtatgcta
     4561 ataatgcagt gatttcccat gcgaatgcgg ctgcagtata ttttgcagat acaagtggct
     4621 tgaaaatatc taattcagag tttttcaatc ccgaaatatt tcatttattg caaccattag
     4681 aagagggatt aattattgat acagataaac tacccataaa aaatagaacc tccaaatctt
     4741 ttagcaaatt actgtacgac gatgtaacct gtgacaaact gagtgtatca gaaatatccg
     4801 atgggaaact taagagatgc agtaggtgcg gttccgtcac aagagcagga aatataatat
     4861 cgagtgataa gacaattgta cccactagca tccaaaccaa aagatggcca accatgtata
     4921 caagactgtg catatgttca ggtatgcttt ttgagatgga cggctgatta ttagtactat
     4981 agcatagttt tgtatagaat tgttttaaac atttcacttc tcgacttaat aaaaagaaat
     5041 gagagtgaca gtgtctggaa tttctttctt ctagaatacc aagggtacgc atttctctcc
     5101 tacggaattt tctgtgcaga tgtcccgatc tacgtggcag atagtgaaaa ctccgtaggc
     5161 aaaaggggca tcggaaaaac ctcttcaaaa aaaaaggttt ttaggctcga acgagccgga
     5221 gcaataatgt tcctttgaaa aatagcaatc gttatgcaac cattttatat gagtctgccc
     5281 ctcaagggac tcgccaccgg accgctgcag ttgtttgcat tcatccacat ataagctggt
     5341 tgtgtatgac aggagaaaaa atacttcatt cgcagttgtt aaccaacagc gacatgtcta
     5401 gcgggaatgt ccaccatacg aaaccgatga tgtacaacgt aaccctgcct tcctacaaca
     5461 gcagtagtat aggtccagtc gataatttga aaataaacga gagacctggc tctcatgatc
     5521 attccatgag aagtgaaata tcatccaaaa ctccggaagc gatttttatg cccacaaatt
     5581 ctattttcac gttccgaagt agtgtctacc aggtcaagat agatcgcggt gactcccgaa
     5641 tacagaggat tcgactttaa gttatgcaga gatc
//