HDTenEightyP/NetNews huggingface dataset:
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human chromosome 4 sequence-tagged site STS4-355
Date: 21 Aug 92 10:09:19 GMT
Distribution: bionet
Checksum: 19502     3

LOCUS       HUM4STS355    430 bp ds-DNA             PRI       21-AUG-1992
DEFINITION  Human chromosome 4 sequence-tagged site STS4-355
ACCESSION   L00812
KEYWORDS    STS; human chromosome 4; sequence tagged site.
SOURCE      Homo sapiens, clone p4-318 from a chromosome 4 plasmid library;
            vector pBluescript II KS + (Stratagene)
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 430)
  AUTHORS   Goold,R.D., diSibio,G., Dugaiczyk,A., Smith,K.A., Xu,H.,
            Magrane,G., Lang,D.B., Cox,D.R., Masters,S.B. and Myers,R.M.
  TITLE     Sequence-Tagged Sites for Human Chromosome 4
  JOURNAL   Unpublished (1992) see COMMENT for author address
  STANDARD  full automatic
COMMENT     PCR components: 25 ng of human genomic DNA, 10 pmol of each
            oligonucleotide, 200 micro-M dNTPs, 0.25 U Taq  polymerase (Cetus)
            in 10 micro-l of 50 mM KCl-20 mM  Tris-HCl, pH 8.3 (at room temp),
            2.5 mM MgCl-2.  Initial denaturation at 94degC for 1.5 min, then 30
            cycles of  94degC for 15 sec, 62degC for 23 sec, and 72degC for 30
            sec, followed by a final extension at 72degC for 3.5 min, using a
            Perkin-Elmer 9600 thermocycler. PCR-amplified product size 236 bp.
            Sequence submitted by:
            Human Genome Mapping Center
            Box 0925
            University of California San Francisco
            San Francisco, CA 94143-0925  USA
            Phone: (415) 502-1612   Fax: (415) 476-8391
            e-mail: hgmcprobes@cgl.ucsf.edu
FEATURES             Location/Qualifiers
     misc_feature    16..38
                     /label=Primer1
     misc_feature    complement(228..251)
                     /label=Primer2
BASE COUNT       88 a     89 c    127 g    120 t      6 others
ORIGIN
        1 gatcgcaggt gcaaacagcc tgtaggacca gctttagaaa aagccatgtc acaaagacac
       61 gtggcatttg acgtgcacat cagcattaga gaccaaaagg gactctgtgt ctgtaggact
      121 ggagaaggta tgacctgtct aaggaagacc ccctagtgcc agttgttggt ttgtttgtgt
      181 gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtntgt tttgagatgg
      241 agtctggctc tgttacccag gctggagtgc agtggcacga ttgcagctca ctgaaacctt
      301 ggcctccagg gttcaagtga ttcccctgcc tcagnctcct gngtagctgg gactacagnc
      361 atgcgccacc aagcccagnt aatttntgta tttttagcaa cagggtttcg ccatgttgac
      421 caggctggtc
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Trypanosoma rangeli trans-spliced leader sequence and 5S rRNA gene.
Date: 19 Aug 92 10:04:05 GMT
Distribution: bionet
Checksum: 45604     3

LOCUS       TRBSPLICE     916 bp ds-DNA             INV       19-AUG-1992
DEFINITION  Trypanosoma rangeli trans-spliced leader sequence and 5S rRNA gene.
ACCESSION   M62864
KEYWORDS    5S rRNA; trans-spliced leader RNA.
SOURCE      Trypanosoma rangeli (strain San Augustin) DNA.
  ORGANISM  Trypanosoma rangeli
            Eukaryota; Animalia; Protozoa; Sarcomastigophora; Mastigophora;
            Kinetoplastida; Trypanosomatina; Trypanosomatidae.
REFERENCE   1  (sites)
  AUTHORS   Aksoy,S., Shay,G.L., Villanueva,M., Beard,C.B. and Richards,F.F.
  TITLE     Spliced leader RNA sequences of Trypanosoma rangeli are encoded
            within the 5S rRNA genes
  JOURNAL   Gene 113, 239-243 (1992)
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     repeat_unit     1..913
                     /note="trans_spliced leader repeat unit"
     CDS             252..349
                     /transl_except=(pos:252..254,aa:Met)
                     /note="trans-spliced leader RNA coding sequence"
                     /codon_start=1
                     /translation="MDPSASYCDPHLRKTPGRGGRDRFQLTLLLIQ"
                     /partial
     rRNA            734..853
                     /note="5S rRNA"
BASE COUNT      207 a    225 c    263 g    221 t
ORIGIN
        1 gggtgtcgta ctctttcatg actatttttt tcaccccaca cacattgcct ttttaaaacc
       61 cacgcacgca cacgcatgca cattgtcggc cccccgcgcc ttattttttc tcccccagcg
      121 cacgccgccc aagaggcaaa cggcatatac acagcccccg cggtaacaat aagcgggggg
      181 ggggagggaa agggcgcggc cgctgcgccc gccgtgctca ccgaaagaag gggagtgtga
      241 ggcgtggaaa agtggacccg tccgcatcct attgtgatcc ccatcttcgc aaaacaccgg
      301 gcaggggcgg gagggatcgc tttcaactaa cgctattatt gatacagttt ctgtactata
      361 ttggtatgca gcgcttccgg atgcgtccga agtcgtgtct gtaatttgga gaggtctcga
      421 cctttttttt tgtcattttt tcttttgatg aatgcgcttg cagggtggta gttgcctctg
      481 ggcttgggaa ccggtgtggg ggtggcggcc gttgcgccca ttcgtttgtc cacacgcaag
      541 cgttgggcac acacacacac acacacacac atatatatat atatatgtat gtatgtatgt
      601 atattacaaa tgcacaagct gctgctatgt attattatgt aattggtgta tgcgtattgt
      661 tacctattac tatatataga gagcgcattg tgcaagatga ggcgtggctc tgcacaacgc
      721 cgtaggggtg gagggaggga gggagggaaa gggggagggc tagggagaga gcgcccagct
      781 ctctcaacac tgaaaacggg aggagtgggt acgaccatac ttggccgaat gcaccacatc
      841 ccgtccgatt tgtgaagtta agcgcacagg cctcgttagt acggcgatca gtgatggcgc
      901 tggaacccga cccggg
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Mouse 19 kDa protein gene, complete cds, clone D4S234E.
Date: 14 Aug 92 10:01:00 GMT
Distribution: bionet
Checksum: 55287     2

LOCUS       MUSPPIA       576 bp ds-DNA             ROD       14-AUG-1992
DEFINITION  Mouse 19 kDa protein gene, complete cds, clone D4S234E.
ACCESSION   M98530
KEYWORDS    19 kDa protein.
SOURCE      Mus musculus DNA.
  ORGANISM  Mus musculus
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Rodentia; Myomorpha; Muridae; Murinae.
REFERENCE   1  (bases 1 to 576)
  AUTHORS   Carlock,L., Vo,T., Wisniewski,D. and Lorincz,M.
  TITLE     The Identification of a neuron-specific gene that maps adjacent to
            the Huntington's Disease marker D4S10 that shows homology to
            protein phosphatase inhibitors
  JOURNAL   Unpublished (1992)
  STANDARD  full staff_entry
FEATURES             Location/Qualifiers
     CDS             1..558
                     /function="unknown"
                     /product="19kDa protein"
                     /gene="19 kDa protein"
                     /codon_start=1
                     /translation="MVKLGNNFAEKGTKQPLLEDGFDTIPLMTPLDVNQLQFPPPDKV
                     VVKTKTEYEPDRKKGKARPPKIAEFTVSITEGVTERFKVSVLVLFALAFLTCVVFLVV
                     YKVYKYDRAYPDGFVLKNTQCIPEGLESYYTEQDSSDREKFYTVINHYKLAKQSITRS
                     VSPWMSVLSEEKLSEQETEAAEKSA"
BASE COUNT      150 a    153 c    152 g    121 t
ORIGIN
        1 atggtgaagt tggggaataa tttcgcagag aagggcacca agcagccact gctggaggat
       61 ggcttcgaca ccattccttt gatgacgccc ctcgatgtca accagctgca gttcccaccc
      121 ccagataagg tcgtggtgaa aactaagact gaatatgaac ctgatcgcaa aaaaggaaaa
      181 gcacgtcctc ccaagatagc cgagttcacc gtcagcatca ccgagggtgt caccgagagg
      241 tttaaggtct ccgtgctggt cctctttgcc ctggccttcc tcacctgtgt cgtcttcctg
      301 gttgtctaca aagtgtacaa gtatgaccgc gcctaccctg atgggtttgt cttgaagaac
      361 acccagtgca tcccagaagg cttggagagc tactacacgg agcaagactc cagtgaccgg
      421 gagaaatttt acactgtcat aaaccactac aagctggcca agcagagcat cacgcgctcc
      481 gtgtcgccat ggatgtcagt tctgtcagaa gagaagctgt cggaacagga gaccgaagct
      541 gcagagaagt cagcttagca agcggggcag gttcct
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Mus musculus interleukin 3 (IL3) gene with an intracisternal A
Date: 19 Aug 92 10:02:51 GMT
Distribution: bionet
Checksum: 23622     3

LOCUS       MUSIL3GAG    1003 bp ds-DNA             ROD       19-AUG-1992
DEFINITION  Mus musculus interleukin 3 (IL3) gene with an intracisternal A
            particle (gag) gene insertion.
ACCESSION   M99279
KEYWORDS    interleukin 3; intracisternal A particle.
SOURCE      Mus musculus (strain BALB/cFull) (library: lambda EMBL3) fetus
            liver DNA.
  ORGANISM  Mus musculus
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Rodentia; Myomorpha; Muridae; Murinae.
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Algate,P.A. and McCubrey,J.A.
  TITLE     Autocrine transformation of hemopoietic cells resulting from
            cytokine message stabilization after IAP transposition
  JOURNAL   Unpublished (1992)
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     cellular        1..243
                     /organism="Mus musculus"
                     /gene="IL3"
                     /citation=[1]
     3'UTR           1..243
                     /gene="IL3"
                     /note="putative"
     LTR             244..577
                     /note="provirus 5' LTR"
                     /note="putative"
     polyA_signal    504..509
                     /note="putative"
     repeat_region   578..911
     CDS             912..>1003
                     /note="proviral intracisternal A particle"
                     /product="gag polyprotein"
                     /note="putative"
                     /codon_start=1
                     /translation="MFGLKFFLVLEALLFLFTCYIVLKIGLKIL"
BASE COUNT      266 a    215 c    224 g    298 t
ORIGIN
        1 tctagaccac ctcagcccgc atctggctcc gtctctccta accgtggaac cgtggaatgt
       61 taaaacagca ggcagagcac ctaaagtctg aatgttcctc atggcccatg gtcaaaagga
      121 ttttacattc ctttatgcca tcaaatgtct tatcaattta tctactttct gaaatttgca
      181 actctccttt ggctttacct aattatgttc ctattttatt ccattaaggc tatttattta
      241 tgtgtgggaa gccgccccca cattcgccgt cacaagatgg cgctgacatc ctgtgttcta
      301 agttggtaaa caaataatct gcgcatgagc caagggtatt tacgaccact tgtactctgt
      361 ttttcccgtg aacgtcagct cggccatggg ctgcagcaat cagggagtga tgcgccctag
      421 gcaatggttg ttctctttaa aatagaaggg gtttcgtttt tctctctctc ttgcttcttg
      481 ctctcttttc ctgaagatgt aagaataaag ctttgtcgca gaagattctg gtctgtggtg
      541 ttcttcctgg ccggtcgtga gaacgcgtcg aataacaatt ggtgccgaat tccgggacga
      601 gaaaatccgg gacgagaaaa aactccggac tggcgcagga gggatacctc attccagaac
      661 cagaactgcg aatcaaggtt ataaggttcc cgtaacacag actgttgaga aggattcaac
      721 tgccgaattc agaactcatc agctggggaa cgacggtgat aaaggttccc gtaaagcaga
      781 ctgttaagaa ggattcaact gtatgaattc agaacttttc agctggggaa cgaggtaagt
      841 ctgatcttga actttctaag gaaattcaag acagtctatc agaagtaaag tggaaaatgg
      901 ctttacaagt tatgtttggc cttaaatttt ttctagtgtt agaagccctt ttgttccttt
      961 tcacatgtta tatagtgctt aagatagggc tgaaaattct aga
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Pterospora andromedea 28S ribosomal RNA, partial.
Date: 14 Aug 92 10:01:11 GMT
Distribution: bionet
Checksum: 32811     2

LOCUS       PSXRR28S      502 bp ss-rRNA            RNA       14-AUG-1992
DEFINITION  Pterospora andromedea 28S ribosomal RNA, partial.
ACCESSION   M98772
KEYWORDS    28S ribosomal RNA.
SOURCE      Pterospora andromedea rRNA.
  ORGANISM  Pterospora andromedea
            Eukaryota; Plantae; Embryobionta; Magnoliophyta; Magnoliopsida;
            Dilleniidae; Ericales; Ericaceae.
REFERENCE   1  (bases 1 to 502)
  AUTHORS   Cullings,K.W.
  TITLE     Design and testing of a plant-specific PCR primer for ecological
            and evolutionary studies
  JOURNAL   Mol. Ecol. (1992) In press
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     rRNA            <1..>502
                     /product="28S ribosomal RNA"
                     /gene="28S rRNA"
                     /note="putative"
                     /citation=[1]
BASE COUNT      116 a    111 c    171 g    102 t      2 others
ORIGIN
        1 gggcgagaga ccgatagcaa acaagtaccg cgaggaaaga tgaaaaggnc tttgaaaaga
       61 gagtcaaaaa gtgcttgaaa ttgtcgggag ggaagcgaat gggggtcggc gatgtgcctc
      121 ggtcggatgt ggaacggtta tagccggtct gccaattgac tcggggtgtg gatcggtgcg
      181 gattgggacg gaggccaaag cccaggaagt cgttaaancc tgtggagacg ccttcgcccc
      241 aattgtggct ggcagcgtgc gtctttatgg cgtgcttcgg catctgtgcg ctcccggcac
      301 cgatcagtgg gctctccatt cggcccgtct tgaaacacgg accaaggagt ctgacatgtg
      361 tgcgagtcaa cgggtgagta aactcgtaag gcgtaaggaa gctgattggt gggatcccct
      421 agtgggttgc accaccgacc gaccgacctt gatcatttga gaagggttcg agtgtgagca
      481 tgcctgtcgg gacccgaaag at
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human chromosome 4 sequence-tagged site STS4-399
Date: 21 Aug 92 10:09:52 GMT
Distribution: bionet
Checksum: 36376     3

LOCUS       HUM4STS399    453 bp ds-DNA             PRI       21-AUG-1992
DEFINITION  Human chromosome 4 sequence-tagged site STS4-399
ACCESSION   L00839
KEYWORDS    STS; human chromosome 4; sequence tagged site.
SOURCE      Homo sapiens, clone p4-388 from a chromosome 4 plasmid library;
            vector pBluescript II KS + (Stratagene)
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 453)
  AUTHORS   Goold,R.D., diSibio,G., Dugaiczyk,A., Smith,K.A., Xu,H.,
            Magrane,G., Lang,D.B., Cox,D.R., Masters,S.B. and Myers,R.M.
  TITLE     Sequence-Tagged Sites for Human Chromosome 4
  JOURNAL   Unpublished (1992) see COMMENT for author address
  STANDARD  full automatic
COMMENT     PCR components: 25 ng of human genomic DNA, 10 pmol of each
            oligonucleotide, 200 micro-M dNTPs, 0.25 U Taq  polymerase (Cetus)
            in 10 micro-l of 50 mM KCl-20 mM  Tris-HCl, pH 8.3 (at room temp),
            2.5 mM MgCl-2.  Initial denaturation at 94degC for 1.5 min, then 30
            cycles of  94degC for 15 sec, 62degC for 23 sec, and 72degC for 30
            sec, followed by a final extension at 72degC for 3.5 min, using a
            Perkin-Elmer 9600 thermocycler. PCR-amplified product size 176 bp.
            Sequence submitted by:
            Human Genome Mapping Center
            Box 0925
            University of California San Francisco
            San Francisco, CA 94143-0925  USA
            Phone: (415) 502-1612   Fax: (415) 476-8391
            e-mail: hgmcprobes@cgl.ucsf.edu
FEATURES             Location/Qualifiers
     misc_feature    36..61
                     /label=Primer1
     misc_feature    complement(187..211)
                     /label=Primer2
BASE COUNT      156 a     85 c     82 g    121 t      9 others
ORIGIN
        1 gatctgtact tcatgattat tatataagtg attccaaaaa gataagttct aatgtgagac
       61 gtngagtttg aactcctcca gaggattaca gccatctcta ttaaactctt tatagcagtt
      121 atttataaac tgacaagggg caagtgttta caggccattt ataatcttct gttgtatctt
      181 tcctnatgac atcacagaaa aatgcatcca actgtgctaa antgtttnca tgaacacaaa
      241 gaatacacac acacactcac acacacactc actcacacag gggcatacac aggcaaacac
      301 atggggaagg tttcccangg ttgggaagta gtagagatat gctattgaaa ctgaaattac
      361 acanagaatg tctggcctat cttgtggcga gtaanctacc atggatgatt taanataaaa
      421 tagaagagat caaagatgct tanaactggc aca
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human chromosome 4 sequence-tagged site STS4-425
Date: 21 Aug 92 10:10:10 GMT
Distribution: bionet
Checksum: 58276     3

LOCUS       HUM4STS425    450 bp ds-DNA             PRI       21-AUG-1992
DEFINITION  Human chromosome 4 sequence-tagged site STS4-425
ACCESSION   L00851
KEYWORDS    STS; human chromosome 4; sequence tagged site.
SOURCE      Homo sapiens, clone p4-479 from a chromosome 4 plasmid library;
            vector pBluescript II KS + (Stratagene)
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 450)
  AUTHORS   Goold,R.D., diSibio,G., Dugaiczyk,A., Smith,K.A., Xu,H.,
            Magrane,G., Lang,D.B., Cox,D.R., Masters,S.B. and Myers,R.M.
  TITLE     Sequence-Tagged Sites for Human Chromosome 4
  JOURNAL   Unpublished (1992) see COMMENT for author address
  STANDARD  full automatic
COMMENT     PCR components: 25 ng of human genomic DNA, 10 pmol of each
            oligonucleotide, 200 micro-M dNTPs, 0.25 U Taq  polymerase (Cetus)
            in 10 micro-l of 50 mM KCl-20 mM  Tris-HCl, pH 8.3 (at room temp),
            2.5 mM MgCl-2.  Initial denaturation at 94degC for 1.5 min, then 30
            cycles of  94degC for 15 sec, 62degC for 23 sec, and 72degC for 30
            sec, followed by a final extension at 72degC for 3.5 min, using a
            Perkin-Elmer 9600 thermocycler. PCR-amplified product size 163 bp.
            STS sequence contains a simple sequence tandem repeat (CA).
            Sequence submitted by:
            Human Genome Mapping Center
            Box 0925
            University of California San Francisco
            San Francisco, CA 94143-0925  USA
            Phone: (415) 502-1612   Fax: (415) 476-8391
            e-mail: hgmcprobes@cgl.ucsf.edu
FEATURES             Location/Qualifiers
     misc_feature    5..27
                     /label=Primer1
     misc_feature    complement(142..167)
                     /label=Primer2
BASE COUNT      134 a     74 c    103 g    131 t      8 others
ORIGIN
        1 gatctccagt ttgcagacag cagattgtgg gacttttcag cctccataat catgtaagcc
       61 aatccaattc ctcctaaaat ctgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt
      121 atgaccaata cacattttat tggtttatac actctatata cacttcataa ttgaacttga
      181 ttggntctat ttctctggag aacccttact aatacatntg aaaacattat gctaagtgaa
      241 ataagccaat cacacacaca aaaatactgt atgattacac ttatatgagg gtacctaaag
      301 gaataaaatt catagacaaa gaaagtagaa ttggtgctta ccaggggctg gggtgagaga
      361 gaaaagagga gtnnggatta atgggtctta gaatttcaga tntggaaagt gaaaggttcc
      421 acttngtcct ngagcggcnc ccgcgggggg
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: S.lividus gene for 16S rRNA (partial)
Date: 22 Aug 92 10:01:49 GMT
Distribution: bionet
Checksum: 05591     2

LOCUS       SYO16SRRN3    233 bp ds-DNA             BCT       22-AUG-1992
DEFINITION  S.lividus gene for 16S rRNA (partial)
ACCESSION   X67093
KEYWORDS    16S rRNA; rRNA.
SOURCE      Synechococcus lividus DNA.
  ORGANISM  Synechococcus lividus
            Prokaryotae; Gracilicutes; Oxyphotobacteria; Cyanobacteria;
            Chroococcales.
REFERENCE   1  (bases 1 to 233)
  AUTHORS   Ward,D.M., Weller,R. and Bateson,M.M.
  TITLE     16S rRNA sequences reveal uncultured inhabitants of a well-studied
            thermal community
  JOURNAL   FEMS Microbiol. Rev. 75, 105-116 (1990)
  STANDARD  full automatic
COMMENT     *source: strain=y-7c7b-S; *source: isolate=Clearwater Spring,
            Yellowstone National Park, USA;
            From EMBL    entry SL16SRRN3;  dated 08-JUL-1992.
BASE COUNT       39 a     50 c     65 g     30 t     49 others
ORIGIN
        1 gggcnctctn aggnnactgc hggggannac hcggaggaag gnnggnatna cgtnaagtcn
       61 tnntngccnn nntncnnggg ctacacacgt ncthnhcnnh ngggggtaca nngggtnnga
      121 achnchnggg ngngctnatc ccagaaagcc cnnctcagtt cggatcgggg tctgcaactc
      181 gaccccgtga agccggaatc gctngtaatg gcggatcagc angccgcngt naa
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Lentinus kauffmanii ribosomal RNA (35LR3) large subunit, partial.
Date: 19 Aug 92 10:02:12 GMT
Distribution: bionet
Checksum: 53795     2

LOCUS       LENRRLSL      214 bp ss-rRNA            RNA       19-AUG-1992
DEFINITION  Lentinus kauffmanii ribosomal RNA (35LR3) large subunit, partial.
ACCESSION   M98596
KEYWORDS    large subunit ribosomal RNA.
SOURCE      Lentinus kauffmannii (library: Duke culture collection 619) cDNA to
            rRNA.
  ORGANISM  Lentinus kauffmannii
            Eukaryota; Fungi; Eumycota; Basidiomycotina; Hymenomycetes;
            Agaricales; Tricholomataceae.
REFERENCE   1  (bases 1 to 214)
  AUTHORS   Hibbett,D.S. and Vilgalys,R.
  TITLE     Phylogenetic relationships of the basidiomycete genus Lentinus
            inferred from molecular and morphological characters
  JOURNAL   Unpublished (1992)
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     rRNA            <1..>214
                     /note="Aligns to region between positions 371 to 597
                     inSaccharomyces cerevisiae large subunit nuclear rRNA."
                     /product="large subunit ribosomal RNA"
                     /citation=[1]
BASE COUNT       46 a     46 c     67 g     55 t
ORIGIN
        1 gaaagagagt taaacagtac gtgaaattgt tgaaagggaa acgcttgaag tcagtcgcgt
       61 tagccggaac tcagccttgc cttccgcttg gtgtattttc cggttgacgg gcccatcgat
      121 tttggctgct ggacaagggc tagcggaatg tggcaccttc gggtgtgtta tagccgctgg
      181 tcgcatacag tagttgggat cggaccgcag cttc
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human chromosome 4 sequence-tagged site STS4-251
Date: 21 Aug 92 10:08:14 GMT
Distribution: bionet
Checksum: 10176     3

LOCUS       HUM4STS251    423 bp ds-DNA             PRI       21-AUG-1992
DEFINITION  Human chromosome 4 sequence-tagged site STS4-251
ACCESSION   L00765
KEYWORDS    STS; human chromosome 4; sequence tagged site.
SOURCE      Homo sapiens, clone p4-196 from a chromosome 4 plasmid library;
            vector pBluescript II KS + (Stratagene)
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 423)
  AUTHORS   Goold,R.D., diSibio,G., Dugaiczyk,A., Smith,K.A., Xu,H.,
            Magrane,G., Lang,D.B., Cox,D.R., Masters,S.B. and Myers,R.M.
  TITLE     Sequence-Tagged Sites for Human Chromosome 4
  JOURNAL   Unpublished (1992) see COMMENT for author address
  STANDARD  full automatic
COMMENT     PCR components: 25 ng of human genomic DNA, 10 pmol of each
            oligonucleotide, 200 micro-M dNTPs, 0.25 U Taq  polymerase (Cetus)
            in 10 micro-l of 50 mM KCl-20 mM  Tris-HCl, pH 8.3 (at room temp),
            2.5 mM MgCl-2.  Initial denaturation at 94degC for 1.5 min, then 30
            cycles of  94degC for 15 sec, 64degC for 23 sec, and 72degC for 30
            sec, followed by a final extension at 72degC for 3.5 min, using a
            Perkin-Elmer 9600 thermocycler. PCR-amplified product size 149 bp.
            Sequence submitted by:
            Human Genome Mapping Center
            Box 0925
            University of California San Francisco
            San Francisco, CA 94143-0925  USA
            Phone: (415) 502-1612   Fax: (415) 476-8391
            e-mail: hgmcprobes@cgl.ucsf.edu
FEATURES             Location/Qualifiers
     misc_feature    55..78
                     /label=Primer1
     misc_feature    complement(179..203)
                     /label=Primer2
BASE COUNT      123 a     88 c    104 g    105 t      3 others
ORIGIN
        1 gatccattga gtccccaaat ccatcctctg gttatataca aagtggcttt gntgagattc
       61 aaattcaggg catccaacat agaggctgtg ctcttactca tgaaacattc tgacactagt
      121 aaccaattta aaaatgcaaa cacctcctgg ggctagccag agtcctccaa acagtcacgt
      181 aaattggttc tgtcaaggat ttcctcctac accctgctga gagccagttg caaggagagg
      241 ctagggaagg gcattgggta actttgtttc taaaagctct tttggataaa gacgtttggg
      301 aaaagaagca aatagagtcc agcagaagag gtaagaaagt aagtttatgt ttggncaggc
      361 acggtggctc acanctgtaa tcccaggact ttggggaggc cgaggtgggc agatccacta
      421 gtt
//