From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Aspergillus niger var awamori pH 2.5 optimum acid phosphatase (aph)
Date: 29 Dec 92 01:26:52 GMT
Distribution: bionet
Checksum: 38074 5

LOCUS       ASN25AP      2071 bp ds-DNA             PLN       08-DEC-1992
DEFINITION  Aspergillus niger var awamori pH 2.5 optimum acid phosphatase (aph)
            gene, complete cds.
ACCESSION   L02420
KEYWORDS    acid phosphatase.
SOURCE      Aspergillus niger (strain ALK0243, sub_species awamori) DNA.
  ORGANISM  Aspergillus niger
            Eukaryota; Fungi; Eumycota; Ascomycotina; Plectomycetes;
            Eurotiales; Trichocomaceae.
REFERENCE   1  (sites)
  AUTHORS   Piddington,C.S., Houston,C.S., Paloheimo,M.T., Cantrell,M.A.,
            Miettinen-Oinonen,A., Nevalainen,H. and Rambosek,J.A.
  TITLE     The cloning and sequencing of the genes encoding phytase (phy) and
            pH 2.5 optimum acid phosphatase (aph) from Aspergillus niger var
            awamori
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
REFERENCE   2  (bases 1 to 2071)
  AUTHORS   Carter,J.R., Franden,M.A., Aebersold,R.H. and McHenry,C.S.
  TITLE     Molecular cloning, sequencing and overexpression of the gene
            encoding the psi subunit of E. coli DNA polymerase III holoenzyme
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     exon            136..916
                     /evidence="EXPERIMENTAL"
                     /citation=[2]
     exon            971..1088
     exon            1141..1245
     exon            1305..1740
     CDS             join(136..916,971..1088,1141..1245,1305..1740)
                     /EC_number="3.1.3.2"
                     /standard_name="pH 2.5 optimum acid phsphatase; pH 2.5 AP"
                     /gene="aph"
                     /product="acid phosphatase"
                     /codon_start=1
                     /translation="MPRTSLLTLACALATGASAFSYGAAIPQSTQEKQFSQEFRDGYS
                     ILKHYGGNGPYSERVSYGIARDPPTSCEVDQVIMVKRHGERYPSPSAGKDIEEALAKV
                     YSINTTEYKGDLAFLNDWTYYVPNECYYNAETTSGPYAGLLDAYNHGNDYKARYGHLW
                     NGETVVPFFSSGYGRVIETARKFGEGFFGYNYSTNAALNIISESEVMGADSLTPTCDT
                     DNDQTTCDNLTYQLPQFKVAAARLNSQNPGMNLTASDVYNLMVMASFELNARPFSNWI
                     NAFTQDEWVSFGYVEDLNYYYCAGPGDKNMAAVGAVYANASLTLLNQGPKEAGSLFFN
                     FAHDTNITPILAALGVLIPNEDLPLDRVAFGNPYSIGNIVPMGGHLTIERLSCQATAL
                     SDEGTYVRLVLNEAVLPFNDCTSGPGYSCPLANYTSILNKNLPDYTTTCNVSASYPQY
                     LSFWWNYNTTTELNYRSSPIACQEGDAMD"
BASE COUNT      458 a    642 c    481 g    490 t
ORIGIN      
        1 gcatgctgga ccgcaatctc cgatcgccgg gtataaaagg tcctccaaac ccctctcggt
       61 cgatatgtac cccgctcgtc atctccaatc ctctcgagag caccttctcc agcttttgtc
      121 aattgtacct tcgcaatgcc tcgcacctct ctcctcaccc tggcctgtgc tctggccacg
      181 ggcgcatccg ctttctccta cggcgctgcc attcctcagt caacccagga gaagcagttc
      241 tctcaggagt tccgcgatgg ctacagcatc ctcaagcact acggtggtaa cggaccctac
      301 tccgagcgtg tgtcctacgg tatcgctcgc gatcccccga ccagctgcga ggtcgatcag
      361 gtcatcatgg tcaagcgtca cggagagcgc tacccgtccc cttcagccgg caaggacatc
      421 gaagaggccc tggccaaggt ctacagcatc aacactactg aatacaaggg cgacctggcc
      481 ttcctgaacg actggaccta ctacgtccct aatgagtgct actacaacgc cgagaccacc
      541 agcggcccct acgccggttt gctggacgcg tacaaccatg gcaacgatta caaggctcgc
      601 tacggccacc tctggaacgg tgagacggtc gtgcccttct tttctagtgg ctacggacgt
      661 gtcatcgaga cggcccgcaa gttcggtgag ggtttctttg gctacaacta ctccaccaac
      721 gctgccctca acatcatctc cgagtccgag gtcatgggcg cggacagcct cacgcccacc
      781 tgtgacaccg acaacgacca gaccacctgc gacaacctga cttaccagct gccccagttc
      841 aaggtcgctg ctgcccgcct aaactcccag aaccccggca tgaacctcac cgcatctgat
      901 gtctacaacc tgatgggtat gtgattacgg tacaatcatt ggctcaaacc tccagctgac
      961 agcatcctag ttatggcctc ctttgagctc aatgctcgtc ccttctccaa ctggatcaac
     1021 gcctttaccc aggacgaatg ggtcagcttc ggttacgttg aggatttgaa ctactactac
     1081 tgcgctgggt gagtttacca tttgatccat tattgtcttg gatcagctaa cgatcgatag
     1141 tcccggtgac aagaacatgg ctgctgtggg tgccgtctac gccaacgcca gtctcaccct
     1201 cctgaaccag ggacccaagg aagccggctc cttgttcttc aacttgtacg ttctcggcag
     1261 aatcagagtc tcacaaaaag aaactcttca ctaacatata gtagtgccca cgacaccaac
     1321 atcaccccca tcctcgccgc cctaggcgtc ctcatcccca acgaggacct tcctcttgac
     1381 cgggtcgcct tcggcaaccc ctactcgatc ggcaacatcg tgcccatggg tggccatctg
     1441 accatcgagc gtctcagctg ccaggccacc gccctctcgg acgagggtac ctacgtgcgt
     1501 ctggtgctga acgaggctgt actccccttc aacgactgca cctccggacc gggctactcc
     1561 tgccctctgg ccaactacac ctccatcctg aacaagaatc tgccagacta cacgaccacc
     1621 tgcaatgtct ctgcgtccta cccgcagtat ctgagcttct ggtggaacta caacaccacg
     1681 acggagctga actaccgctc tagccctatt gcctgccagg agggtgatgc tatggactag
     1741 atgcagaggg gtaggtcccg ggatacttta gtgatgattg atattcaagt ttggtggtga
     1801 cgatcacctt gttaatagtc ttgtacagtc atacggtgaa tgtaaataat gataatagca
     1861 atgatacatg ttggaatctc gttttgttct ttgtgtgcat aggcgctttg ggggtgtatt
     1921 tttaggcgtt agacttattt tcaattcgtg tataatgcgg tcagtaaatg aatcatcaat
     1981 tattcaaatg caatgctgta tacgtgaaac tattgggtta agacgcagct actagctgac
     2041 tgcttggtta ctttctgtgt acaccgcatg c
//