HDTenEightyP/NetNews huggingface dataset:
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: M.musculus rearranged TCR delta gene D-delta-2 region
Date: 25 Jul 92 10:04:53 GMT
Distribution: bionet
Checksum: 43584     2

LOCUS       MUSTCRD2      133 bp ds-DNA             ROD       25-JUL-1992
DEFINITION  M.musculus rearranged TCR delta gene D-delta-2 region
ACCESSION   X64901
KEYWORDS    D-region; T cell receptor; antigen receptor; delta gene.
SOURCE      Mus musculus DNA.
  ORGANISM  Mus musculus
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Rodentia; Myomorpha; Muridae; Murinae.
REFERENCE   1  (bases 1 to 133)
  AUTHORS   Chien,Y.H., Iwashima,M., Wettstein,D.A., Kaplan,K.B., Elliott,J.F.,
            Born,W. and Davis,M.M.
  TITLE     T-cell receptor delta gene rearrangements in early thymocytes
  JOURNAL   Nature 330, 24-31 (1987)
  STANDARD  full automatic
COMMENT     *source: stage=18-19 day fetus; *source: cell_type=thymocytes;
            *source: clone=12;
            See also X63933-9 & X64900-3
            From EMBL    entry MMTCRD2;  dated 25-JUN-1992.
FEATURES             Location/Qualifiers
     misc_feature    54..69
                     /note="D delta 2 region"
BASE COUNT       40 a     33 c     36 g     24 t
ORIGIN
        1 gacacgtgat acaaagccca gggaaggttt ttgcaaagct ctgtagcacc gtgatcggag
       61 ggatacgagc acagtgttgc aaaccccata gggacctgta caaaaactgc agtgaagaac
      121 gtctcccagg cct
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: H.sapiens gamma-delta T-cell Vgamma9+ mRNA
Date: 28 Jul 92 10:05:04 GMT
Distribution: bionet
Checksum: 24595     2

LOCUS       HUMS2TB12B    222 bp ss-RNA             PRI       28-JUL-1992
DEFINITION  H.sapiens gamma-delta T-cell Vgamma9+ mRNA
ACCESSION   Z11409
KEYWORDS    T-cell; T-cell Vgamma9+.
SOURCE      Homo sapiens RNA.
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 222)
  AUTHORS   Holroyd,K.
  JOURNAL   Unpublished (1991)
  STANDARD  full automatic
REFERENCE   2  (sites)
  AUTHORS   Holroyd,K.J., Maeda,H., Kirby,M. and Crystal,R.G.
  TITLE     Vgamma9+ mRNA sequences from sarcoidosis gamma-delta T-cell lines
            responsive to Mycobacterium tuberculosis in vitro are oligoclonal
            and overlap with Vgamma9+ mRNA sequences from autologous blood...
  JOURNAL   Unpublished (1992)
  STANDARD  full staff_review
COMMENT
            From EMBL    entry HSS2TB12B;  dated 02-JUL-1992.
FEATURES             Location/Qualifiers
     misc_feature    88..98
                     /note="N region insertion"
BASE COUNT       72 a     55 c     39 g     56 t
ORIGIN
        1 ataggatacc tgaaacgtct actaccactc tcaccattca caatgtagag aaacaggaca
       61 tagctaccta ctactgtgcc ttgtgggccc gatacaggga gttgggcaaa aaaatcaagg
      121 tatttggtcc cggaacaaag cttatcatta cagataaaca acttgatgca gatgtttccc
      181 ccaagcccac tatttttctt ccttcaattg ctgaaacaaa ac
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Macaca mulatta (SIV-infected) T-cell receptor beta chain mRNA,
Date: 30 Jul 92 10:01:52 GMT
Distribution: bionet
Checksum: 50566     2

LOCUS       MACTRCG       453 bp ss-mRNA            PRI       30-JUL-1992
DEFINITION  Macaca mulatta (SIV-infected) T-cell receptor beta chain mRNA,
            partial cds.
ACCESSION   M87475
KEYWORDS    T-cell receptor.
SOURCE      Macaca mulatta (individual_isolate 244) peripheral blood cDNA to
            mRNA.
  ORGANISM  Macaca mulatta
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Cercopithecidae;
            Cercopithecinae; Papionini.
REFERENCE   1  (bases 1 to 453)
  AUTHORS   Chen,Z.W., Yamamoto,H., Watkins,D.I., Levinson,G. and Letvin,N.L.
  TITLE     A predominant use of T cell receptor Vb gene family in SIV
            gag-specific cytoxic T lymphocytes
  JOURNAL   J. Virol. 66, 3913-3917 (1992)
  STANDARD  full staff_entry
FEATURES             Location/Qualifiers
     CDS             1..453
                     /product="T-cell receptor beta-chain"
                     /gene="TCR"
                     /codon_start=1
                     /translation="ESPHSCCDPAMDTWLLCWAIFSLLKAGHTEPEVTQTPSHQVTQM
                     GQEVILRCVPIPNHLNFYWYRQILGQKVEFLVIFFDNNISEKSEIFEDRFSVGRPDGS
                     NFTLKIKSTKLEDSAMYFCASSGDRAKDYTFGSGTKLTVVEDLKKVFPP"
                     /partial
BASE COUNT      124 a    109 c    107 g    113 t
ORIGIN
        1 gagagccctc attcctgctg tgatcctgcc atggatacct ggctcctatg ctgggcaatt
       61 tttagtctct tgaaagcagg acacacagaa cctgaagtca cccagactcc cagccatcag
      121 gtcacacaga tgggacagga agtgatcttg cggtgtgtcc ccatccctaa tcacttaaac
      181 ttctattggt acagacaaat cttggggcag aaagtcgagt ttctggttat cttttttgat
      241 aataacatct cagagaagtc tgaaatattt gaagatcgat tctcagtcgg aaggcctgat
      301 ggatcaaatt tcactctgaa gatcaagtcc acaaagctgg aggactcagc catgtacttc
      361 tgtgccagca gcggggacag ggcaaaggac tacaccttcg gttcagggac caagttaact
      421 gttgtagagg acctgaaaaa ggtgttccca ccc
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Babesia bovis probable member of the aldo-keto reductase
Date: 29 Jul 92 10:01:16 GMT
Distribution: bionet
Checksum: 30437     3

LOCUS       BBOALDKET    1003 bp ss-mRNA            INV       29-JUL-1992
DEFINITION  Babesia bovis probable member of the aldo-keto reductase
            superfamily mRNA, 3' end.
ACCESSION   M97631
KEYWORDS    aldo-keto reductase.
SOURCE      Babesia bovis (strain Samford attenuated) cDNA to mRNA.
  ORGANISM  Babesia bovis
            Eukaryota; Animalia; Protozoa; Microspora; Microsporea;
            Piroplasmia; Piroplasmida; Babesiidae.
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Dalrymple,B.P., Peters,J.M. and Vuocolo,T.
  TITLE     The sequence of a Babesia bovis gene encoding a member of a
            putative new branch of the aldo-keto reductase superfamily
  JOURNAL   Unpublished (1992)
  STANDARD  simple staff_entry
FEATURES             Location/Qualifiers
     CDS             1..859
                     /product="aldo-keto reductase"
                     /codon_start=2
                     /translation="SKVVIATKVASRSSHLSWLRSGDCKLSKDNILKAVDGSLSRLNT
                     DYIDLLQLHWPDRYVPMNANGDFHEVFHDTENMIDENSVPLEDQLDALQTLLTQGKIR
                     HWGLSNETPWGTLRFYKLAKQAGVAAPASVQLHYNLLCRNEVEKGFVELCRPQNTGIA
                     ILAYAPLAGGILTGKYLEYMDPTTSGRLLRFPSYMSRYRGSLAARAVKDYYNIAMSYK
                     YPNLCALALRWVLTRPFICSTVIGANDFYQLRENIHCSMPSLGITDLLEREINQLHWK
                     WRDPIRICQ"
                     /partial
BASE COUNT      277 a    202 c    223 g    301 t
ORIGIN
        1 gtcaaaggtt gttatcgcca caaaagttgc tagtaggagt tcacatttat catggctgag
       61 atcaggcgac tgtaagctat cgaaggataa tatattaaag gctgtcgacg ggtctttgtc
      121 tcgtttgaat acagactaca tcgatttatt gcaactacat tggcctgacc gttacgttcc
      181 aatgaatgct aacggggact ttcacgaagt cttccatgat actgagaaca tgatcgatga
      241 gaatagtgtc cctctagaag atcagttaga tgcacttcag acgttactaa cccagggaaa
      301 aattcgacat tggggtttgt ccaatgaaac tccctgggga acgttacgtt tctacaagct
      361 ggcaaaacaa gctggcgtag ctgctccagc atctgtgcaa ttgcattaca atttactttg
      421 ccgaaacgaa gtggaaaagg gatttgtgga attatgtcgt cctcaaaata caggcatcgc
      481 aattctggcc tacgcccccc ttgcgggtgg gatattgact gggaaatatt tggaatatat
      541 ggatccaacg acttcaggtc gcttactaag gttcccatca tatatgtcac gatacagggg
      601 ttcattggct gcacgtgcag tgaaggacta ttacaacatt gcaatgtcgt acaagtaccc
      661 taatttatgt gctcttgcct tacgctgggt gcttacgaga ccattcatct gtagtacagt
      721 aattggcgcg aacgactttt atcaattacg tgaaaacatt cactgctcaa tgccttcttt
      781 gggaatcact gacctactag agcgagagat taaccagctg cattggaagt ggcgtgatcc
      841 aattcgcatt tgtcagtagt atttttttct attggcaaat tccccctttg tttcaaacta
      901 tttgtagcag gaagtgtgct gatatcgcgc tattagttga gtttcgagtc atattaaact
      961 tcgttttaat tcgatgtgta atgttgcgac attttaaaaa aaa
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: H.sapiens gamma-delta T-cell Vgamma9+ mRNA
Date: 28 Jul 92 10:04:44 GMT
Distribution: bionet
Checksum: 36612     2

LOCUS       HUMN3TB16     222 bp ss-RNA             PRI       28-JUL-1992
DEFINITION  H.sapiens gamma-delta T-cell Vgamma9+ mRNA
ACCESSION   Z11278
KEYWORDS    T-cell; T-cell Vgamma9+.
SOURCE      Homo sapiens RNA.
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 222)
  AUTHORS   Holroyd,K.
  JOURNAL   Unpublished (1991)
  STANDARD  full automatic
REFERENCE   2  (sites)
  AUTHORS   Holroyd,K.J., Maeda,H., Kirby,M. and Crystal,R.G.
  TITLE     Vgamma9+ mRNA sequences from sarcoidosis gamma-delta T-cell lines
            responsive to Mycobacterium tuberculosis in vitro are oligoclonal
            and overlap with Vgamma9+ mRNA sequences from autologous blood...
  JOURNAL   Unpublished (1992)
  STANDARD  full staff_review
COMMENT
            From EMBL    entry HSN3TB16;  dated 02-JUL-1992.
FEATURES             Location/Qualifiers
     misc_feature    91..94
                     /note="N region insertion"
BASE COUNT       73 a     55 c     39 g     55 t
ORIGIN
        1 ataggatacc tgaaacgtct actaccactc tcaccattca caatgtagag aaacaggaca
       61 tagctaccta ctactgtgcc ttgtgggagg caccgcaaga gttgggcaaa aaaatcaagg
      121 tatttggtcc cggaacaaag cttatcatta cagataaaca acttgatgca gatgtttccc
      181 ccaagcccac tatttttctt ccttcaattg ctgaaacaaa ac
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Fusobacterium nucleatum small subunit rRNA sequence.
Date: 29 Jul 92 10:01:57 GMT
Distribution: bionet
Checksum: 27926     3

LOCUS       FUSSRRNAE    1522 bp ss-rRNA            RNA       29-JUL-1992
DEFINITION  Fusobacterium nucleatum small subunit rRNA sequence.
ACCESSION   M58683
KEYWORDS    ribosomal RNA small subunit.
SOURCE      Fusobacterium nucleatum (library: ATCC 25586) rRNA.
  ORGANISM  Fusobacterium nucleatum
            Prokaryotae; Gracilicutes; Scotobacteria;
            Anaerobic gram-negative straight, curved and helical rods;
            Bacteroidaceae.
REFERENCE   1  (bases 1 to 1522)
  AUTHORS   Paster,B.J., Dewhirst,F.E., Yang,D., Weisburg,W.G. and Woese,C.R.
  TITLE     Phylogeny of the fusobacteria and relatives
  JOURNAL   Unpublished (1992)
  STANDARD  full staff_entry
BASE COUNT      411 a    299 c    437 g    347 t     28 others
ORIGIN
        1 attgaacgaa gagtttgatc ctggctcagg atgaacgctg acagaatgct taacacatgc
       61 aagtctactt gaatttgggt tttttaactt cgatttgggt ggcggacggg tgagtaacgc
      121 gtaaagaact tgcctcacag ctagggacaa catttggaaa cgaatgctaa tacctaatat
      181 tatgattata gggcatccta gaattatgaa agctatatgc gctgtgagag agctttgcgt
      241 cccattagct agttggagag gtaacggctc accaaggcaa tgatgggnag ccggcctgag
      301 agggtgaacg gccacaaggg gactgagaca cggcccttac tcctacggga ggcagcagtg
      361 gggaatattg gacaatggac cgagagtctg atccagcaat tctgtgtgca cgatgacgtt
      421 tttcggaatg taaagtgctt tcagttggga agaaaaaaat gacggtacca acagaagaag
      481 tgacggctaa atacgtgcca gcagccgcgg taatacgtat gtcacgagcg ttatccggat
      541 ttattgggcg taaagcgcgt ctaggtggtt atgtaagtct gatgtgaaaa tgcagggctc
      601 aactctgtat tgcgttggaa actgtgtaac tagagtnctg gaaaggtaag cggaactaca
      661 agtgtagagg tgaaattcgt agatatttgt aggaatgccg atggggaagc cagcttactg
      721 gacagatact gacgctgaag cgcnnnagcg tgggtagcaa acaggattag ataccctggt
      781 agtccacgcc gtaaacgatg attactaggt gttgggggtc gaacctcagc gcccaagcaa
      841 acgcgataag taatccgcct ggggagtacg tacgcaagta tgaaactcaa aggaattgac
      901 ggggacccgc acaagcggtg gagcatgtgg tttaattcga ngcaacgcga ggaaccttac
      961 cagcgtttga catcttagga atgagacaga gatgtttcag tgtcccttcg gggaaaccta
     1021 aagacaggtg gtgcatggct gtcgtcagct cgtgtcgtga gatgttgggt taagtcccgc
     1081 aacgagcgca acccctttcg tatgttacca tcattaagtt ggggactcat gcgatactgc
     1141 ctacgatgag taggaggaag gtggggatga cgtcaagtca tcatgcccct tatacgctgg
     1201 gctacacacg tgctacaatg ggtagaacag agagttgcaa agccgtgagg tggagctaat
     1261 ctcagaaaac tattcttagt tcggattgta ctctgcaact cgagtacatg aagttggaat
     1321 cgctagtaat cgcgaatcag caatgtcgcg gtgaatacgt tctcgggtct tgtacacacc
     1381 gcccgtcaca ccacgagagt tggttgcacc tgaagtagca ggcctaaccg taaggaggga
     1441 tgttccgagg gtgtgattag cgannggggn nnnnnngtaa caaggtatcc gnnnnngaac
     1501 gnnnnnnnng atcacctcct tt
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: H.sapiens gamma-delta T-cell Vgamma9+ mRNA
Date: 28 Jul 92 10:04:21 GMT
Distribution: bionet
Checksum: 48555     2

LOCUS       HUMN3B8       219 bp ss-RNA             PRI       28-JUL-1992
DEFINITION  H.sapiens gamma-delta T-cell Vgamma9+ mRNA
ACCESSION   Z11252
KEYWORDS    T-cell; T-cell Vgamma9+.
SOURCE      Homo sapiens RNA.
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 219)
  AUTHORS   Holroyd,K.
  JOURNAL   Unpublished (1991)
  STANDARD  full automatic
REFERENCE   2  (sites)
  AUTHORS   Holroyd,K.J., Maeda,H., Kirby,M. and Crystal,R.G.
  TITLE     Vgamma9+ mRNA sequences from sarcoidosis gamma-delta T-cell lines
            responsive to Mycobacterium tuberculosis in vitro are oligoclonal
            and overlap with Vgamma9+ mRNA sequences from autologous blood...
  JOURNAL   Unpublished (1992)
  STANDARD  full staff_review
COMMENT
            From EMBL    entry HSN3B8;  dated 02-JUL-1992.
FEATURES             Location/Qualifiers
     misc_feature    91..92
                     /note="N region insertion"
BASE COUNT       73 a     53 c     38 g     55 t
ORIGIN
        1 ataggatacc tgaaacgtct actaccactc tcaccattca caatgtagag aaacaggaca
       61 tagctaccta ctactgtgcc ttgtgggagg accaagagtt gggcaaaaaa atcaaggtat
      121 ttggtcccgg aacaaagctt atcattacag ataaacaact tgatgcagat gtttccccca
      181 agcccactat ttttcttcct tcaattgctg aaacaaaac
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: M.musculus rearranged TCR delta gene V-region, D-delta-2 &
Date: 25 Jul 92 10:05:01 GMT
Distribution: bionet
Checksum: 01862     2

LOCUS       MUSTCRVDJ3    286 bp ds-DNA             ROD       25-JUL-1992
DEFINITION  M.musculus rearranged TCR delta gene V-region, D-delta-2 &
            J-delta-1 regions (clone M16)
ACCESSION   X63937
KEYWORDS    D-region; J-region; T cell receptor; V-region; antigen receptor;
            delta gene.
SOURCE      Mus musculus DNA.
  ORGANISM  Mus musculus
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Rodentia; Myomorpha; Muridae; Murinae.
REFERENCE   1  (bases 1 to 286)
  AUTHORS   Chien,Y.H., Iwashima,M., Wettstein,D.A., Kaplan,K.B., Elliott,J.F.,
            Born,W. and Davis,M.M.
  TITLE     T-cell receptor delta gene rearrangements in early thymocytes
  JOURNAL   Nature 330, 24-31 (1987)
  STANDARD  full automatic
COMMENT     *source: stage=18-19 day fetus; *source: cell_type=thymocytes;
            *source: clone=M16;
            See also X63933-9 & X64900-3
            From EMBL    entry MMTCRVDJ3;  dated 25-JUN-1992.
FEATURES             Location/Qualifiers
     misc_feature    1..238
                     /note="V region"
     misc_feature    239..249
                     /note="D delta 2 region"
     misc_feature    250..286
                     /note="J delta 1 region"
BASE COUNT       99 a     53 c     65 g     69 t
ORIGIN
        1 ggatcctgcc tccttctact gcactgtaac aggaggggac atgaagaatt atcatatgag
       61 ctggtataag aagaatggaa ctaatgctct gtttttagta tacaagctaa atagcaattc
      121 tactgatggt ggaaagagca acctcaaagg gaaaattaac atttcaaaaa atcagtttat
      181 actcgacatt cagaaggcaa caatgaaaga cgctggtacg tactactgtg ggtcagatcg
      241 gagggatacc gacaaactcg tctttggaca aggaacccaa gtgact
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: H.sapiens gamma-delta T-cell Vgamma9+ mRNA
Date: 28 Jul 92 10:03:41 GMT
Distribution: bionet
Checksum: 62741     2

LOCUS       HUMN1TB34     216 bp ss-RNA             PRI       28-JUL-1992
DEFINITION  H.sapiens gamma-delta T-cell Vgamma9+ mRNA
ACCESSION   Z11199
KEYWORDS    T-cell; T-cell Vgamma9+.
SOURCE      Homo sapiens RNA.
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 216)
  AUTHORS   Holroyd,K.
  JOURNAL   Unpublished (1991)
  STANDARD  full automatic
REFERENCE   2  (sites)
  AUTHORS   Holroyd,K.J., Maeda,H., Kirby,M. and Crystal,R.G.
  TITLE     Vgamma9+ mRNA sequences from sarcoidosis gamma-delta T-cell lines
            responsive to Mycobacterium tuberculosis in vitro are oligoclonal
            and overlap with Vgamma9+ mRNA sequences from autologous blood...
  JOURNAL   Unpublished (1992)
  STANDARD  full staff_review
COMMENT
            From EMBL    entry HSN1TB34;  dated 02-JUL-1992.
FEATURES             Location/Qualifiers
     misc_feature    93..96
                     /note="N region insertion"
BASE COUNT       69 a     53 c     38 g     56 t
ORIGIN
        1 ataggatacc tgaaacgtct actaccactc tcaccattca caatgtagag aaacaggaca
       61 tagctaccta ctactgtgcc ttgtgggagg tgctcgtggg caaaaaaatc aaggtatttg
      121 gtcccggaac aaagcttatc attacagata aacaacttga tgcagatgtt tcccccaagc
      181 ccactatttt tcttccttca attgctgaaa caaaac
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human mRNA sequence.
Date: 21 Jul 92 10:06:05 GMT
Distribution: bionet
Checksum: 04908     1

LOCUS       HUMRTPGEAX    207 bp ss-mRNA            PRI       21-JUL-1992
DEFINITION  Human mRNA sequence.
ACCESSION   M91505
KEYWORDS    sequence tagged site.
SOURCE      Homo sapiens cDNA to mRNA.
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 207)
  AUTHORS   Swaroop,A. and Gieser,L.
  TITLE     Expressed sequence tags and chromosomal localization of cDNA clones
            from a subtracted retinal pigment epithelium library
  JOURNAL   Genomics 13, 873-876 (1992)
  STANDARD  full automatic
BASE COUNT       44 a     44 c     59 g     55 t      5 others
ORIGIN
        1 ttcgtactta tncaggnaga tggcattgat ctctgaaggg tnaagcagga ggtcatagtt
       61 natgtcaact tgttgaacat ccagcccagg gagtncttgc agatgggcag gatgctagtg
      121 tcaaacctgc cttgggctgt gttggagctg gtgggcttat gactctgttc gcatctcgtg
      181 gagagctcca aaccaatcct tcagccg
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: M.musculus rearranged TCR delta gene V-region, D-delta-2 &
Date: 25 Jul 92 10:05:03 GMT
Distribution: bionet
Checksum: 19801     2

LOCUS       MUSTCRVDJ5    356 bp ds-DNA             ROD       25-JUL-1992
DEFINITION  M.musculus rearranged TCR delta gene V-region, D-delta-2 &
            J-delta-1 (clone M23)
ACCESSION   X63939
KEYWORDS    D-region; J-region; T cell receptor; V-region; antigen receptor;
            delta gene.
SOURCE      Mus musculus DNA.
  ORGANISM  Mus musculus
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Rodentia; Myomorpha; Muridae; Murinae.
REFERENCE   1  (bases 1 to 356)
  AUTHORS   Chien,Y.H., Iwashima,M., Wettstein,D.A., Kaplan,K.B., Elliott,J.F.,
            Born,W. and Davis,M.M.
  TITLE     T-cell receptor delta gene rearrangements in early thymocytes
  JOURNAL   Nature 330, 24-31 (1987)
  STANDARD  full automatic
COMMENT     *source: stage=18-19 day fetus; *source: cell_type=thymocytes;
            *source: clone=M23;
            See also X63933-9 & X64900-3
            From EMBL    entry MMTCRVDJ5;  dated 25-JUN-1992.
FEATURES             Location/Qualifiers
     misc_feature    16..305
                     /note="V region"
     misc_feature    306..316
                     /note="D delta 2 region"
     misc_feature    317..356
                     /note="J delta 1"
BASE COUNT      102 a     80 c     83 g     91 t
ORIGIN
        1 gcaggatcta atgtggccca gaaagtgatt caggtctggt caacaacaag caggcaggag
       61 ggagaaaaac tcacactgga ctgttcatat aagacaagtc aggtcttata ccatcttttc
      121 tggtacaagc accttcttag tggagagatg gttttgctta ttcgacaaat gccttctact
      181 attgcaatag agaggagcgg ccgctattct gtagtcttcc agaaatcacg caaatccatc
      241 agccttgtca tttcaacctt acaaccagac gattcgggaa agtatttctg tgctctctgg
      301 gagctggagg gatacgtacc gacaaactcg tctttggaca aggaacccaa gtgact
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: H.sapiens dystrophin exon 6 PCR primer, 6F
Date: 24 Jul 92 10:07:09 GMT
Distribution: bionet
Checksum: 17980     2

LOCUS       HUMPCR6F       27 bp ds-DNA             PRI       24-JUL-1992
DEFINITION  H.sapiens dystrophin exon 6 PCR primer, 6F
ACCESSION   X65345
KEYWORDS    PCR.
SOURCE      Homo sapiens DNA.
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 27)
  AUTHORS   Beggs,A.H., Koenig,M., Boyce,F.M. and Kunkel,L.M.
  TITLE     Detection of 98% of DMD/BMD gene deletions by polymerase chain
            reaction
  JOURNAL   Hum. Genet. 86, 45-48 (1990)
  STANDARD  full automatic
COMMENT     *source: allele=DMD/BMD; *source: tissue=peripheral blood; *source:
            cell_type=leukocyte; *source: chromosome=X;
            PCR reactions using reagents from Perkin-Elmer Cetus GeneAmp kits
            (Norwalk, Conn.) contained 0.5ul of each primer (e.g. ~0.5uM), 5
            units Taq polymerase and 250ng genomic DNA per 50ul reaction. In
            practice, all primers for a given reaction were generally premixed
            and stored in aliquots. Each set of reactions was always prepared
            as a master mix and aliquots added to 0.5ml microfuge tubes before
            addition of water and template to a final volume of 50ul. These
            were overlain with 25ul mineral oil and incubated in either a
            Perkin-Elmer Cetus (Norwalk, Conn.) thermal cycler or an MJ
            Research (Waterton, Mass.) thermal controller as follows: 94oC,
            7min to denature, followed by 25 cycles of 94oC, 30s (to denature);
            65oC, 4min (to anneal and elongate) with the last
            annealing/elongation step for 10min. PCR products were stored at
            4oC for up to several days prior to analysis.
            From EMBL    entry HSPCR6F;  dated 12-MAY-1992.
BASE COUNT       12 a      4 c      5 g      6 t
ORIGIN
        1 ccacatgtag gtcaaaaatg taatgaa
//