From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Escherichia coli K12 W3110 Asp-tRNA.
Date: 29 Jul 92 10:01:44 GMT
Distribution: bionet
Checksum: 00063     9

LOCUS       ECOD12650    3929 bp ds-DNA             BCT       29-JUL-1992
DEFINITION  Escherichia coli K12 W3110 Asp-tRNA.
ACCESSION   D12650
KEYWORDS    .
SOURCE      Escherichia coli DNA.
  ORGANISM  Escherichia coli
            Prokaryotae; Gram-negative facultatively anaerobic rods;
            Enterobacteriaceae.
REFERENCE   1  (bases 1 to 3929)
  AUTHORS   Nishimura,K., Komine,Y., Miyamoto,K., Kitabatake,M., Mathunaga,F.,
            Hisano,T., Miki,T. and Inokuchi,H.
  TITLE     Nucleotide sequence of the 3'flanking region of the ribosomal RNA
            gene (rrnH) in E.coli
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
COMMENT     Submitted (17-JUL-1992) to DDBJ by:
            Koichi Nishimura
            Dept. of Biophysics, Faculty of Science
            Kyoto University
            Kitashirakawa-oiwake-cho
            Sakyo-ku, Kyoto 606-01
            Japan
            Phone:  075-753-4200
            Fax:    075-791-0271
FEATURES             Location/Qualifiers
     tRNA            10..86
                     /product="'Asp-tRNA'"
     CDS             249..1052
                     /product="ORF267"
                     /codon_start=1
                     /translation="MAIPAFGLGTFRLKDDVVISSVITALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIAAYAAKHNATPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
     CDS             complement(1049..1963)
                     /note="'stop codons of ORF267 and ORF304 overlap each
                     other'"
                     /product="ORF304"
                     /codon_start=1
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHICLGFTEPASLNTWPIARSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPGGAVREA"
     CDS             2225..3004
                     /product="ORF259"
                     /codon_start=1
                     /translation="MRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERIRILVWNI
                     YKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFVLPQHPSG
                     VMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNFSLGVDVY
                     SKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTDDQRRRAF
                     GRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     CDS             3008..3631
                     /product="ORF207"
                     /codon_start=1
                     /translation="MSGLPQGRPTFGAAQNVSAVVAYDLSAHMLDVVAQAAEARQLKN
                     ITTRQGYAESLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRLIVMDVMSP
                     GHPVRDIWLQTVEALRDTSHVRNYASGEWLTLINEANLIVDNLITDKLPLEFSSWVAR
                     MRTPEALVDAIRIYQQSASTEVRTYFALQNDGFFTSDIIMVDAHKAA"
BASE COUNT      991 a    947 c    959 g   1032 t
ORIGIN
        1 gaattcggtg gagcggtagt tcagtcggtt agaatacctg cctgtcacgc agggggtcgc
       61 gggttcgagt cccgtccgtt ccgccactta ttaagaagcc tcgagttaac gctcgaggtt
      121 ttttttcgtc tgtatatcta ttattgccag aatcgcaaaa atcctctgca ttttacgctc
      181 tttttcctca acagtctgaa gcccataatc acctcagtta acgaaaatag cattaaaaga
      241 ggcatattat ggctatccct gcatttggtt taggtacttt ccgtctgaaa gacgacgttg
      301 ttatttcatc tgtgataacg gcgcttgaac ttggttatcg cgcaattgat accgcacaaa
      361 tctatgataa cgaagccgca gtaggtcagg cgattgcaga aagtggcgtg ccacgtcatg
      421 aactctacat caccactaaa atctggattg aaaatctcag caaagacaaa ttgatcccaa
      481 gtctgaaaga gagcctgcaa aaattgcgta ccgattatgt tgatctgacg ctaatccact
      541 ggccgtcacc aaacgatgaa gtctctgttg aagagtttat gcaggcgctg ctggaagcca
      601 aaaaacaagg gctgacgcgt gagatcggta tttccaactt cacgatcccg ttgatggaaa
      661 aagcgattgc tgctgttggt gctgaaaaca tcgctactaa ccagattgaa ctctctcctt
      721 atctgcaaaa ccgtaaagtg gttgcctggg ctaaacagca cggcatccat attacttcct
      781 atatgacgct ggcgtatggt aaggccctga aagatgaggt tattgctgcg tatgcagcta
      841 aacacaatgc gactccggcc caagtgattc tggcgtgggc tatgggggaa ggttactcag
      901 taattccttc ttctactaaa cgtaaaaacc tggaaagtaa tcttaaggca caaaatttac
      961 agcttgatgc cgaagataaa aaagcgatcg ccgcactgga ttgcaacgac cgcctggtta
     1021 gcccggaagg tctggctcct gaatgggatt aagcctctct gacagctcct ccgggagctg
     1081 tttttacatg ctcgctaagg aaatcgataa aagcccggat gcgcgtactt accgcacggt
     1141 cgctgtaata gacggcactg aatggcattt ccactggcaa cactttatct gccattaact
     1201 ccaccaattc tccgcgagcg atttctttgt cgatcatgta gtcggacaaa cacgcaatcc
     1261 cgttgccact caggcaaagc tgtttcagtg tttccccact attggatgac aaaccgtact
     1321 tcacctcatg taattgtcca tcgctacggg ctatcggcca ggtattgagg gaagcgggtt
     1381 cagtgaatcc caggcaaata tgttgcttta aatcgtcgat cgtttctggc ttcccgtagc
     1441 gggaaatata atcgggggag gcgataattt ttcgataact gttaaataac ggcctggcac
     1501 gtaagctgga atccgttaac gtaccagcgc gtatcgcgac atccactttt ctttcgatca
     1561 aattaataat cgtttcggag gagactagcg acaaagtgac ttccggatag cgttcacgga
     1621 aaggcttaat taacggcatc agaaagtgca gcaccactgg agttgcggca tcgatccgta
     1681 acagtccacg cggtgtatta cgcgtctcca taatttctga ttctgccgct gccatctcct
     1741 gcaaaattga ctgtacgcga cgaaaataac gctcgccttc ttccgtcagg ctaagttgtc
     1801 gcgtggtccg attaagcagg ctaacgccaa gtttcatctc cagctttttc accgcccggc
     1861 ttaccgctga gtttgcttgc cctaattgtt ccgctgcccg gctaaagctg ccgctttcga
     1921 cgaccgaaac aaaaatggcg agttcttccg acgtggcttt catttttgct cctgttgcaa
     1981 aatagaagag atattttgaa tttatttgtc attaaaccat caggatgcgt gatatgtcat
     2041 gcgatttaat gttctccata atgagcaaaa ttctgaccgg tgtaagcact tgcttacata
     2101 acaatataca attgctcgtt gaaagagtga gctaaaatcc ctataacagt agaaccctcc
     2161 cgagtgcgga agggttgacg taatagaggt ttcaaagtca aaagtgcgaa aaaacaccta
     2221 tgccatgcgc tatgttgccg gacaacctgc ggaaaggatc ttaccgccgg ggtcttttgc
     2281 gagcatcggc caggcattac cacctgggga accgttaagt accgaagagc gtattcggat
     2341 cctggtgtgg aacatataca aacagcaacg cgctgaatgg ttgtcggtat taaagaacta
     2401 cggcaaagat gcacatctgg tgttattgca ggaagcgcag acaacgccag agttagtaca
     2461 gtttgcgacc gctaactatc ttgccgccga tcaggtaccc gctttcgtgc tgccacaaca
     2521 tccttctggc gtaatgaccc tttcggcggc acatccagtg tattgctgcc cgttacgtga
     2581 acgagaaccc attttgcgtc tggcgaagtc ggcactggtg acggtctatc cattacctga
     2641 cacccgcctg ttgatggtgg ttaatataca cgccgtcaac ttcagtctgg gcgtggatgt
     2701 ctatagtaag cagttacttc ctattggcga tcagatagct caccacagcg gcccggtcat
     2761 tatggcggga gatttcaatg cctggagccg tagaaggatg aacgcgttat atcgctttgc
     2821 gcgggaaatg tcgctgcgcc aggtgcgttt taccgatgat cagcgccgtc gggcgtttgg
     2881 tcgcccgctc gattttgttt tctaccgtgg tctgaacgtc agtgaagctt ctgtactggt
     2941 tacgcgcgct tccgatcaca atccgctact cgttgaattc agtcccggca agcctgataa
     3001 ataaggtatg tcaggtctgc cacagggcag accaacgttt ggcgctgcgc aaaacgtgag
     3061 cgcggtggtg gcgtatgact tatctgccca catgctggat gtcgtggcac aagctgccga
     3121 agcccggcaa ctgaaaaata tcaccacccg ccagggatat gccgaaagtc tgccatttgc
     3181 cgataacgca tttgatattg ttatcagccg ttattctgcc catcactggc atgatgttgg
     3241 tgcagcactg cgagaagtga ataggatatt gaaacctggc ggtaggctga ttgtgatgga
     3301 cgtaatgtct ccgggtcacc cagtgcgcga catctggtta cagacggtag aagcattacg
     3361 cgatacctct cacgtacgaa actacgccag cggtgagtgg ttgacgttaa tcaatgaagc
     3421 caatctgata gttgataatt taattacaga taagttaccg ctggaatttt cttcatgggt
     3481 cgcgagaatg cgtacgccag aagcgttagt agacgctatt cgcatttacc aacagagcgc
     3541 atcgacagag gtgagaacgt attttgcctt gcagaatgat ggctttttca ccagtgatat
     3601 catcatggta gatgcacata aagcggcata aataaaaaag gcaccggggg aatcggtgcc
     3661 tttttattat ctggtttgtc aggaatctgg catgttgttg tttttcacaa acaacgtcag
     3721 cttatcgcct ggttgcagat tcgcagtgtc gctgttccag cgcatcacat ctttgatgtc
     3781 acgccgtggc gtttagcaat gcttgaaagc gaatcgcctt tgcgcacacg atacgtaatg
     3841 ctatcgctgt tgtttgccag tcgctgtgcg ctactgcctg caccaatcgt caaactttgg
     3901 cctggcttca gcttagatcc ggggaattc
//