From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Oryzias latipes hatching enzyme constituent protease (hce) mRNA,
Date: 3 Jan 93 22:18:16 GMT
Distribution: bionet
Checksum: 07071 4

LOCUS       ORZHCEA       910 bp ss-mRNA            VRT       23-DEC-1992
DEFINITION  Oryzias latipes hatching enzyme constituent protease (hce) mRNA,
            complete cds.
ACCESSION   M96170
KEYWORDS    hatching enzyme; protease; zinc metalloprotease.
SOURCE      Oryzias latipes pre-hatching embryo exocrine gland cDNA to mRNA.
  ORGANISM  Oryzias latipes
            Eukaryota; Animalia; Chorqata; Vertebrata; Osteichthyes;
            Actinopterygii; Atheriniformes; Cyprinodontoidei; Oryziatidae.
REFERENCE   1  (bases 1 to 910)
  AUTHORS   Yasumasu,S., Yamada,K., Akasaka,K., Mitsunaga,K., Iuchi,I.,
            Shimada,H. and Yamagami,K.
  TITLE     Isolation of cDNAs for LCE and HCE, two constituent proteases of
            the hatching enzyme of Oryzias Latipes, and concurrent expression
            of their mRNAs during development
  JOURNAL   Dev. Biol. 153, 250-258 (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     sig_peptide     26..85
                     /gene="hce"
                     /codon_start=1
     misc_feature    86..235
                     /gene="hce"
                     /note="propeptide"
     misc_feature    182..184
                     /gene="hce"
                     /note="glycosylation site"
     mat_peptide     236..835
                     /gene="hce"
                     /codon_start=1
     misc_binding    530..532
                     /bound_moiety="zinc"
                     /gene="hce"
     misc_feature    533..535
                     /gene="hce"
                     /note="active site"
     misc_binding    542..545
                     /bound_moiety="zinc"
                     /gene="hce"
     polyA_signal    879..884
                     /gene="hce"
     CDS             26..838
                     /gene="hce"
                     /product="protease"
                     /codon_start=1
                     /translation="MNLAPSTCLLLLFLLDIAQALPVWDEEGHEEGHEEGDGDDFVDI
                     TTRILTSNNNTDQLLLEGDLVAPTNRNAMKCWSSSCFWKKASNGLVVIPYVISSEYSG
                     GEVATIEGAMRAFNGKTCIRFVRRTNEYDFISVVSKTGCYSELGRKGGQQELSINRGG
                     CMYSGIIQHELNHALGFQHEQTRSDRDSYVRINWENIIPASAYNFNKHDTNNLNTPYD
                     YSSIMHYGRDAFSIAYGRDSITPIPNPNVPIGQRNGMSRWDITRINVLYNCR"
BASE COUNT      267 a    247 c    204 g    192 t
ORIGIN      
        1 cagttctgaa ccctccacat ccaccatgaa cttggctccc tccacctgcc tgcttttact
       61 gttcctgctc gacattgctc aggcactgcc tgtctgggac gaagaaggcc atgaagaggg
      121 ccatgaagaa ggtgatggag atgactttgt agacatcact accaggatcc tcaccagcaa
      181 caataacact gaccagctct tgctagaagg agacctggtg gctcctacaa acaggaatgc
      241 catgaaatgc tggagcagca gctgtttctg gaagaaagcc tccaacggtt tagtggtaat
      301 tccttacgtc attagctctg agtactctgg aggagaagtg gccaccatcg aaggcgccat
      361 gagggctttt aacggaaaaa catgcatccg ctttgtccgt cgcaccaacg agtacgactt
      421 cattagcgtt gtgagcaaaa ctggatgcta ctctgagctg ggcaggaagg gaggacaaca
      481 ggagctgtct atcaacaggg gaggatgcat gtacagcggc atcatccaac acgagctcaa
      541 ccacgctctg ggcttccagc atgaacagac caggagcgac cgagacagct acgtccggat
      601 caactgggag aacatcatcc cagcgagtgc ctacaatttt aacaagcatg ataccaacaa
      661 cctgaacacc ccctacgact actcctccat catgcactat ggaagagatg ccttctccat
      721 tgcctatggg cgagattcca tcacccccat ccccaatccc aatgtcccca tcggccagag
      781 gaatggcatg tcccgctggg acatcaccag aatcaacgtc ctttacaact gtcgttaaaa
      841 atgtttatgt caagatctat aatgtctcaa ttactacaaa taaagtaaat gcaaagcaaa
      901 aaaaaaaaaa
//