From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human carbamyl phosphate synthetase I (EC 6.3.4.16) mRNA.
Date: 21 Jul 92 10:05:22 GMT
Distribution: bionet
Checksum: 27399    10

LOCUS       HUMCPSI      5215 bp ss-mRNA            PRI       21-JUL-1992
DEFINITION  Human carbamyl phosphate synthetase I (EC 6.3.4.16) mRNA.
ACCESSION   D90282
KEYWORDS    carbamyl phosphate synthetase I; urea cycle.
SOURCE      Human liver, cDNA to mRNA.
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 5215)
  AUTHORS   Haraguchi,Y., Uchino,T., Takiguchi,M., Endo,F., Mori,M. and
            Matsuda,I.
  TITLE     Cloning and sequence of a cDNA encoding human carbamyl phosphate
            synthetase I: molecular analysis of hyperammonemia
  JOURNAL   Gene 107, 335-340 (1991)
  STANDARD  full staff_review
COMMENT     Submitted (18-JAN-1991) to DDBJ by:
            Yougo Haraguchi
            Department of Pediatrics
            Kumamoto University Medical School
            1-1-1 Honjo
            Kumamoto 860
            Japan
            Phone:  096-344-2111 x5654
            Fax:    096-366-3471
FEATURES             Location/Qualifiers
     CDS             119..4621
                     /note="carbamyl phosphate synthetase I (EC 6.3.4.16)"
                     /codon_start=1
                     /translation="MTRILTAFKVVRTLKTGFGFTNVTAHQKWKFSRPGIRLLSVKAQ
                     TAHIVLEDGTKMKGYSFGHPSSVAGEVVFNTGLGGYPEAITDPAYKGQILTMANPIIG
                     NGGAPDTTSLDELGLSKYLESNGIKVSGLLVLDYSKDYNHWLATKSLGQWLQEEKVPA
                     IYGVDTRMLTKIIRDKGTMLGKIEFEGQPVDFVDPNKQNLIAEVSTKDVKVYGKGNPT
                     KVVAVDCGIKNNVIRLLVKRGAEVHLVPWNHDFTKMEYDGILIAGGPGNPALAEPLIQ
                     NVQKILESDRKEPLFGISTGNLITGLAAGAKTYKMSMANRGQNQPVLNITNKQAFITA
                     QNHCYALDNTLPAGWKPLFVNVNDQTNEGIMHESKPFFAVQFHPEVTPGPIDTEYLFD
                     SFFSLIKKGKATTITSVLPKPALVASRVEVSKVLILGSGGLSIGQAGEFDYSGSQAVK
                     AMKEENVKTVLMNPNIASVQTNEVGLKQADTVYFLPITPQFVTEVIKAEQPDGLILGM
                     GGQTALNCGVELFKRGVLKEYGVKVLGTSVESIMATEDRQLFSDKLNEINEKIAPSFA
                     VESIEDALKAADTIGYPVMIRSAYALGGLGSGICPNRETLMDLSTKAFAMTNQILVEK
                     SVTGWKEIEYEVVRDADDNCVTVCNMENVDAMGVHTGDSVVVAPAQTLSNAEFQMLRR
                     TSINVVRHLGIVGECNIQFALHPTSMEYCIIEVNAKMSPNSALASKTTGYPLAFIAAK
                     IALGIPLPGIKNVVSGKTSACFEPSLDYMVTKIPRWDLDRFHGTSSRIGSSMKSVGEV
                     MAIGRTFEESFQKALRMCHPSIEGFTPRLPMNKEWPSNLDLRKELSEPSSTRIYAIAK
                     AIDDNMSLDEIEKLTYIDKWFLYKMRDILNMEKTLKGLNSESMTEETLKRAKEIGFSD
                     KQISKCLGLTEAQTRELRLKKNIHPWVKQIDTLAAEYPSVTNYLYVTYNGQEHDVNFD
                     DHGMMVLGCGPYHIGSSVEFDWCAVSSIRTLRQLGKKTVVVNCNPETVSTDFDECDKL
                     YFEELSLERILDIYHQEACGGCIISVGGQIPNNLAVPLYKNGVKIMGTSPLQIDRAED
                     RSIFSAVLDELKVAQAPWKAVNTLNEALEFAKSVDYPCLLRPSYVLSGSAMNVVFSED
                     EMKKFLEEATRVSQATPVVLTKFVEGAREVEMDAVGKDGRVISHAISEHVEDAGVHSE
                     NATLMLPTQTISQGAIEKVKDATRKIAKAFAISGPFNVQFLVKGNDVLVNECNLRASR
                     SFPSVSKTLGVDFIDVATKVLIGENVDEKHLPTLDHPIIPVDYVAIKAPMFSWPRLRD
                     ADPILRCEMASTGEVACFGEGIHTAFLKAMLSTGFKIPQKGILIGIQQSFRPRFLGVA
                     EQLHNEGFKLFATEATSDWLNANNVPANPVAWPSQEGQNPSLSSIRKLIRDGSIDLVI
                     NLPNNNTKFVHDNYVIRRTAVDSGIPLLTNFQVTKLFAEAVQKSRKVDSKSLFHYRQY
                     SAGKAA"
BASE COUNT     1484 a   1102 c   1207 g   1422 t
ORIGIN      short arm of chromosome 2.
        1 aagcaacctt aaaatgactg caccctccca gatttctttt acattaacta aaaagtctta
       61 tcacacaatc tcataaaatt tatgtaattt catttaattt tagccacaaa tcatcaaaat
      121 gacgaggatt ttgacagctt tcaaagtggt gaggacactg aagactggtt ttggctttac
      181 caatgtgact gcacaccaaa aatggaaatt ttcaagacct ggcatcaggc tcctttctgt
      241 caaggcacag acagcacaca ttgtcctgga agatggaact aagatgaaag gttactcctt
      301 tggccatcca tcctctgttg ctggtgaagt ggtttttaat actggcctgg gagggtaccc
      361 agaagctatt actgaccctg cctacaaagg acagattctc acaatggcca accctattat
      421 tgggaatggt ggagctcctg atactacttc tctggatgaa ctgggactta gcaaatattt
      481 ggagtctaat ggaatcaagg tttcaggttt gctggtgctg gattatagta aagactacaa
      541 ccactggctg gctaccaaga gtttagggca atggctacag gaagaaaagg ttcctgcaat
      601 ttatggagtg gacacaagaa tgctgactaa aataattcgg gataagggta ccatgcttgg
      661 gaagattgaa tttgaaggtc agcctgtgga ttttgtggat ccaaataaac agaatttgat
      721 tgctgaggtt tcaaccaagg atgtcaaagt gtacggcaaa ggaaacccca caaaagtggt
      781 agctgtagac tgtgggatta aaaacaatgt aatccgcctg ctagtaaagc gaggagctga
      841 agtgcactta gttccctgga accatgattt caccaagatg gagtatgatg ggattttgat
      901 cgcgggagga ccggggaacc cagctcttgc agaaccacta attcagaatg ttcagaagat
      961 tttggagagt gatcgcaagg agccattgtt tggaatcagt acaggaaact taataacagg
     1021 attggctgct ggtgccaaaa cctacaagat gtccatggcc aacagagggc agaatcagcc
     1081 tgttttgaat atcacaaaca aacaggcttt cattactgct cagaatcatt gctatgcctt
     1141 ggacaacacc ctccctgctg gctggaaacc actttttgtg aatgtcaacg atcaaacaaa
     1201 tgaggggatt atgcatgaga gcaaaccctt cttcgctgtg cagttccacc cagaggtcac
     1261 cccggggcca atagacactg agtacctgtt tgattccttt ttctcactga taaagaaagg
     1321 aaaagctacc accattacat cagtcttacc gaagccagca ctagttgcat ctcgggttga
     1381 ggtttccaaa gtccttattc taggatcagg aggtctgtcc attggtcagg ctggagaatt
     1441 tgattactca ggatctcaag ctgtaaaagc catgaaggaa gaaaatgtca aaactgttct
     1501 gatgaaccca aacattgcat cagtccagac caatgaggtg ggcttaaagc aagcggatac
     1561 tgtctacttt cttcccatca cccctcagtt tgtcacagag gtcatcaagg cagaacagcc
     1621 agatgggtta attctgggca tgggtggcca gacagctctg aactgtggag tagaactatt
     1681 caagagaggt gtgctcaagg aatatggtgt gaaagtcctg ggaacttcag ttgagtccat
     1741 tatggctacg gaagacaggc agctgttttc agataaacta aatgagatca atgaaaagat
     1801 tgctccaagt tttgcagtgg aatcgattga ggatgcactg aaggcagcag acaccattgg
     1861 ctacccagtg atgatccgtt ccgcctatgc actgggtggg ttaggctcag gcatctgtcc
     1921 caacagagag actttgatgg acctcagcac aaaggccttt gctatgacca accaaattct
     1981 ggtggagaag tcagtgacag gttggaaaga aatagaatat gaagtggttc gagatgctga
     2041 tgacaattgt gtcactgtct gtaacatgga aaatgttgat gccatgggtg ttcacacagg
     2101 tgactcagtt gttgtggctc ctgcccagac actctccaat gccgagtttc agatgttgag
     2161 acgtacttca atcaatgttg ttcgccactt gggcattgtg ggtgaatgca acattcagtt
     2221 tgcccttcat cctacctcaa tggaatactg catcattgaa gtgaatgcca agatgtcccc
     2281 gaactctgct ctggcctcca aaacgactgg ctacccattg gcattcattg ctgcaaagat
     2341 tgccctagga atcccacttc caggaattaa gaacgtcgta tccgggaaga catcagcctg
     2401 ttttgaacct agcctggatt acatggtcac caagattccc cgctgggatc ttgaccgttt
     2461 tcatggaaca tctagccgaa ttggtagctc tatgaaaagt gtaggagagg tcatggctat
     2521 tggtcgtacc tttgaggaga gtttccagaa agctttacgg atgtgccacc catctataga
     2581 gggtttcact ccccgtctcc caatgaacaa agaatggcca tcgaatttag atcttagaaa
     2641 agagttgtct gaaccaagca gcacgcgtat ctatgccatt gccaaggcca ttgatgacaa
     2701 catgtccctt gatgagattg agaagctcac atacattgac aagtggtttt tgtataagat
     2761 gcgtgatatt ttaaacatgg aaaagacact gaaaggcctc aacagtgagt ccatgacaga
     2821 agaaaccctg aaaagggcaa aggagattgg gttctcagat aagcagattt caaaatgcct
     2881 tgggctcact gaggcccaga caagggagct gaggttaaag aaaaacatcc acccttgggt
     2941 taaacagatt gatacactgg ctgcagaata cccatcagta acaaactatc tctatgttac
     3001 ctacaatggt caggagcatg atgtcaattt tgatgaccat ggaatgatgg tgctaggctg
     3061 tggtccatat cacattggca gcagtgtgga atttgattgg tgtgctgtct ctagtatccg
     3121 cacactgcgt caacttggca agaagacggt ggtggtgaat tgcaatcctg agactgtgag
     3181 cacagacttt gatgagtgtg acaaactgta ctttgaagag ttgtccttgg agagaatcct
     3241 agacatctac catcaggagg catgtggtgg ctgcatcata tcagttggag gccagattcc
     3301 aaacaacctg gcagttcctc tatacaagaa tggtgtcaag atcatgggca caagccccct
     3361 gcagatcgac agggctgagg atcgctccat cttctcagct gtcttggatg agctgaaggt
     3421 ggctcaggca ccttggaaag ctgttaatac tttgaatgaa gcactggaat ttgcaaagtc
     3481 tgtggactac ccctgcttgt tgaggccttc ctatgttttg agtgggtctg ctatgaatgt
     3541 ggtattctct gaggatgaga tgaaaaaatt cctagaagag gcgactagag tttctcaggc
     3601 cacgccagtg gtgctgacaa aatttgttga aggggcccga gaagtagaaa tggacgctgt
     3661 tggcaaagat ggaagggtta tctctcatgc catctctgaa catgttgaag atgcaggtgt
     3721 ccactcggag aatgccactc tgatgctgcc cacacaaacc atcagccaag gggccattga
     3781 aaaggtgaag gatgctaccc ggaagattgc aaaggctttt gccatctctg gtccattcaa
     3841 cgtccaattt cttgtcaaag gaaatgatgt cttggtgaat gagtgtaact tgagagcttc
     3901 tcgatccttc ccctctgttt ccaagactct tggggttgac ttcattgatg tggccaccaa
     3961 ggtgttgatt ggagagaatg ttgatgagaa acatcttcca acattggacc atcccataat
     4021 tcctgttgac tatgttgcaa ttaaggctcc catgttttcc tggccccggt tgagggatgc
     4081 tgaccccatt ctgagatgtg agatggcttc cactggagag gtggcttgct ttggtgaagg
     4141 tattcataca gccttcctaa aggcaatgct ttccacagga tttaagatac cccagaaagg
     4201 catcctgata ggcatccagc aatcattccg gccaagattc cttggtgtgg ctgaacaatt
     4261 acacaatgaa ggtttcaagc tgtttgccac ggaagccaca tcagactggc tcaacgccaa
     4321 caatgtccct gccaacccag tggcatggcc gtctcaagaa ggacagaatc ccagcctctc
     4381 ttccatcaga aaattgatta gagatggcag cattgaccta gtgattaacc ttcccaacaa
     4441 caacactaaa tttgtccatg ataattatgt gattcggagg acagctgttg atagtggaat
     4501 ccctctcctc actaattttc aggtgaccaa actttttgct gaagctgtgc agaaatctcg
     4561 caaggtggac tccaagagtc ttttccacta caggcagtac agtgctggaa aagcagcata
     4621 gagatgcaga caccccagcc ccattattaa atcaacctga gccacatgtt atataaagga
     4681 actgattcac aactttctca gagatgaata ttgataacta aacttcattt cagtttactt
     4741 tgttatgcct taatattctg tgtcttttgc aattaaattg tcagtcactt cttcaaaacc
     4801 ttacagtcct tcctaaggtt actcttcatg agattcatcc atttactaat actgtatttt
     4861 tggtggacta ggcttgccta tgtgcttatg tgtagctttt tactttttat ggtgtgatta
     4921 atggtgatca aggtaggaaa agttgtgttc tattttcttg aactccttct atactttaag
     4981 atactctatt tttaaaacac tatctgcaaa ctcaggacac tttaacaggg cagaatactc
     5041 taaaaacttg ataaaattaa atatagattt aatttatgaa ccttccatca tgtgtttgtg
     5101 tattgcttct ttttggatcc tcattctcac ccatttggct aatccaggaa tattgttatc
     5161 ccttcccatt atattgaagt tgagaaatgt gacagagcat ttagagtatg aattc
//