From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Vibrio cholerae transcriptional activator (toxT) gene, complete
Date: 31 Dec 92 01:41:23 GMT
Distribution: bionet
Checksum: 47869 5

LOCUS       VIBTCPFTOX   2175 bp ds-DNA             BCT       17-DEC-1992
DEFINITION  Vibrio cholerae transcriptional activator (toxT) gene, complete
            cds, ORF1 (tcpF) gene, complete cds.
ACCESSION   L01623
KEYWORDS    tcpF gene; toxT gene; transcriptional activator.
SOURCE      Vibrio cholerae (strain 569B) DNA.
  ORGANISM  Vibrio cholerae
            Prokaryotae; Facultatively anaerobic gram-negative rods;
            Vibrionaceae.
REFERENCE   1  (bases 1 to 2175)
  AUTHORS   Higgins,D.E., Nazareno,E. and DiRita,V.J.
  TITLE     The virulence gene activator ToxT from Vibrio cholerae is a member
            of the AraC family of transcriptional activators
  JOURNAL   J. Bacteriol. 174, 6974-6980 (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     CDS             63..1079
                     /gene="tcpF"
                     /codon_start=1
                     /translation="MRYKKTLMLSIMITSFNSFAFNDNYSSTSTVYATSNEATDSRGS
                     EHLRYPYLECIKIGMSRDYLENCVKVSFPTSQDMFYDAYPSTESDGAKTRTKEDFSAR
                     LLAGDYDSLQKLYIDFYLAQTTFDWEIPTRDQIETLVNYANEGKLSTALNQEYITGRF
                     LTKENGRYDIVNVGGVPDNTPVKLPAIVSKRGLMGTTSVVNAIPNEIYPHIKVYEGTL
                     SRLKPGGAMIAVLEYDVNELSKHGYTNLWDVQFKVLVGVPHAETGVIYDPVYEETVKP
                     YQPSNNLTGKKLYNVSTNDMHNGYKWSNTMFSNSNYKTQILLTKGDGSGVKLYSKAYS
                     ENFK"
     CDS             1286..2116
                     /function="AraC-like transcriptional activator of
                     virulence genes in Vibrio cholerae"
                     /gene="toxT"
                     /product="transcriptional activator"
                     /codon_start=1
                     /translation="MIGKKSFQTNVYRMSKFDTYIFNNLYINDYKMFWIDSGIAKLID
                     KNCLVSYEINSSSIILLKKNAIQRFSLTSLSDENINVSVITISDSFIRSLKSYILGDL
                     MIRNLYSENKDLLLWNCEHNDIAVLSEVVNGFREINYSDEFLKVFFSGFFSKVEKKYN
                     SIFITDDLDAMEKISCLVKSDITRNWRWADICGELRTNRMILKKELESRGVKFRELIN
                     SIRISYSISLMKTGEFKIKQIAYQSGFASVSYFSTVFKSTMNVAPSEYLFMLTGVAEK
                     "
BASE COUNT      742 a    297 c    396 g    740 t
ORIGIN      
        1 ggatcttcgg tgcgatcttt tcgctcgttg ataaactaag ttcatcttta tagtgaggat
       61 atatgagata taaaaaaacc ttaatgttat caatcatgat tacatcattt aactcatttg
      121 catttaacga taattatagt tcaaccagta cggtttatgc tacgtctaat gaagctacgg
      181 attcaagggg gagtgagcac cttagatacc cttatttaga gtgtatcaaa atcggtatga
      241 gtagagatta tcttgaaaac tgtgtgaaag tatcttttcc aacctctcaa gatatgtttt
      301 atgacgcata cccatcgaca gaatctgacg gtgctaagac aagaacaaaa gaagatttct
      361 ctgctcggct tttagctggt gattatgata gtttacagaa attgtatata gatttttatc
      421 ttgctcaaac gactttcgat tgggaaatac cgacaaggga tcaaattgag acactagtta
      481 attatgctaa tgaaggtaaa ttgtctacgg cacttaatca agagtatatt acaggtcgat
      541 ttcttacaaa agaaaatggt cgatatgata ttgtgaatgt tggtggtgtt ccagataata
      601 ctccagttaa actaccagct attgtttcta aacgcggact aatgggtaca acttctgtag
      661 ttaatgcaat tcccaatgaa atttatcctc atatcaaagt ttatgaaggg actcttagtc
      721 gtttaaaacc tggtggtgca atgatcgcag tattagaata tgacgtaaat gaattaagta
      781 agcacgggta taccaatctg tgggatgtgc agtttaaggt tcttgttgga gttccacatg
      841 cagaaacagg agttatctat gaccctgttt atgaagaaac tgtcaaacca tatcagccta
      901 gtaacaactt aacgggtaag aagttgtata atgtatctac aaatgacatg cataatggtt
      961 acaagtggtc aaatactatg ttctcaaatt ctaattataa aacgcaaata ttattaacta
     1021 aaggagatgg aagtggtgtg aaactgtata gcaaagcata ttcagagaac tttaaataaa
     1081 atagataaga taacagccac attcgtggct gttatcttat ctcaaaaaac ataaaataac
     1141 atgagttact ttatgttttt cttatgtaat acgtctgtaa cttgttctta tgtctgttat
     1201 gtagttttat attaacttcg attttaagat aactttacgt ggatggctct ctgcgtttat
     1261 ttatatatct tcagagtaga acgcaatgat tgggaaaaaa tcttttcaaa ctaatgtata
     1321 tagaatgagt aaatttgata cttacatatt caataactta tatataaacg actacaaaat
     1381 gttctggata gatagtggaa ttgcaaagct tatagataaa aattgcttgg ttagttatga
     1441 gataaattca agttcaatta tcttactcaa gaaaaacgct attcagcgat tttctttgac
     1501 ttcattatca gatgaaaata taaatgtttc tgtaattaca ataagtgatt catttatacg
     1561 ttcactaaaa tcttacattc ttggtgatct catgataagg aatttatata gtgaaaataa
     1621 agatctatta ctttggaatt gtgaacataa tgatatagct gtcctttctg aagtggtaaa
     1681 tggtttcaga gaaattaatt attcagatga gttcctaaaa gttttttttt cagggttctt
     1741 ctcgaaagta gaaaaaaaat ataactctat atttattact gatgatcttg atgctatgga
     1801 gaaaatttca tgtttagtaa aaagtgatat tacgcgtaat tggcgttggg cagatatttg
     1861 tggtgaatta agaacgaatc ggatgatttt gaaaaaagaa ctagagtctc gaggagtaaa
     1921 gtttagagaa ttaattaata gcattcgaat atcatattca atttcactaa tgaaaaccgg
     1981 tgaattcaaa ataaaacaga ttgcatatca gtctgggttt gctagcgttt catatttttc
     2041 tacagtattt aagtcaacca tgaatgtagc accaagtgaa tatttattta tgttgacagg
     2101 agttgcagaa aaataatgga atacgtttac ttgatcctat tttcgattgt atcactgatt
     2161 ttgggtagtt ttagt
//