From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: S.coelicolor abaA gene
Date: 24 Jul 92 10:10:11 GMT
Distribution: bionet
Checksum: 37797     7

LOCUS       STMABAA      2666 bp ds-DNA             BCT       24-JUL-1992
DEFINITION  S.coelicolor abaA gene
ACCESSION   X60316
KEYWORDS    abaA gene; antibiotic biosynthesis.
SOURCE      Streptomyces coelicolor DNA.
  ORGANISM  Streptomyces coelicolor
            Prokaryotae; Firmicutes; Streptomycetaceae.
REFERENCE   1  (bases 1 to 2666)
  AUTHORS   Fernandez-Moreno,M.A., Martin-Triana,A.J., Martinez,E., Niemi,J.,
            Kieser,H.M., Hopwood,D.A. and Malpartida,F.
  TITLE     abaA, a new pleiotropic regulatory locus for antibiotic production
            in Streptomyces coelicolor
  JOURNAL   J. Bacteriol. 174, 2958-2967 (1992)
  STANDARD  full automatic
COMMENT     *source: strain=ssp.A3, M145;
            From EMBL    entry SCABAA;  dated 13-MAY-1992.
FEATURES             Location/Qualifiers
     CDS             complement(93..428)
                     /note="orfE"
                     /gene="abaA"
                     /codon_start=1
                     /translation="MSAASHRISRLEPYLDRAEPAPTLLKMLVGVQLAGFREDAGLSQ
                     DQAARALGFSAAKLSRIESGKGRRLPRRPTSGRCWSTTTPIRTRPRSCSSCCNERANR
                     AGGSGTTSA"
     RBS             complement(435..438)
                     /note="orfE"
     CDS             complement(555..782)
                     /note="orfD"
                     /gene="abaA"
                     /codon_start=1
                     /translation="MPPVRNGVQASLLDACWKKSRHSNAEGNCVEVALVDGGIAMRNS
                     RDPDGPALVYTPAEVAAFLAGAKDGEFDHLV"
     RBS             complement(788..794)
                     /note="orfD"
     RBS             952..958
                     /note="orfA"
     CDS             963..1541
                     /note="orfA"
                     /gene="abaA"
                     /codon_start=1
                     /translation="MPSPAHPTLRSPGEPGSEAGEATRSGGLPYPVRATAGRAVPPSS
                     APYPGSPALGGRPTAAVLRVACSGEGFARARVFTRDTLRGWSLDHLGDDAVLVITELV
                     SNALTHAVPPSVAGGPEIGLGLALGSGRLKLTVSDPGDDAPRLNPSDGSALREHGRGL
                     CIVDALAEEWGWTPRPPAGKTVWATLSTRPLT"
     CDS             1672..2115
                     /note="orfB"
                     /gene="abaA"
                     /codon_start=1
                     /translation="MPWSDIQDSTGSAAAIPRLLRKVARGDAETARAALGDLRRRICQ
                     YGFVVEQATAATVPFLWELAQRPQVSCRAQIIQLLKNIADARQWETTATAYPKLLNHR
                     ENPVAWERAARQAVRARRDGLERLLADDDTEIMRATSELARTLGD"
     RBS             2259..2265
                     /note="orfC"
     CDS             2274..>2666
                     /note="orfC"
                     /gene="abaA"
                     /codon_start=1
                     /translation="MASSMEKPLDHRYRGEHPIRTLVYLFRADRRRLAGAVAVFTVKH
                     SPIWLLPLVTAAIVDTVVQHGPITDLWTSTGLIMFILVVNYPLHLLYVRLLYGSVRRM
                     GTALRSALCTRMQQLSIGYHSRVSAGVLQ"
BASE COUNT      384 a   1023 c    892 g    367 t
ORIGIN
        1 ctgcagcaga ccggggacgt actggatctc gaaggtacgg atggtcgccg cggcctcctg
       61 aaggccgacc aggcggtcga accactcggg catcaggcgc ttgtcgtacc gctgccacca
      121 gcccggttcg cccgctcgtt gcaacagctt gagcaggacc gaggcctcgt acggatcggt
      181 gtcgtagtgc tccagcagcg cccggacgtc ggtctccgag ggagcctgcg gcccttgccc
      241 gactcgatgc gcgagagctt cgcggcgctg aagccgagcg cgcgtgcggc ctggtcctgg
      301 gagaggccgg catcctcgcg gaagcccgcc agctgcacgc cgacgagcat cttcagcagg
      361 gtcggagccg gctcggcccg atccagatag ggttcgagac gggagatgcg atgcgacgcg
      421 gcggacatcc tgactcccag cagaccggca gacttgaagg aaagactatc tcatcccctc
      481 ggtgcccgcc gcatccgccc gcggaaatcg ggtagttggg ctccgcttcc gggggccgcc
      541 gcgccgcccg cgccctacac caggtggtcg aactcgccgt ccttcgcacc cgccaggaac
      601 gccgccacct cggccggggt gtagacgagc gcggggccgt cggggtcccg ggagttgcgc
      661 atcgcgatgc ctccgtcgac gagggcgacc tcgacgcagt tgccctcggc gttgctgtgc
      721 cgactcttct tccagcaggc gtccaacaag ctggcctgca ctccgttgcg cactggtggc
      781 accgcggtct ccttgctgtt cgggggagcg gaccgagtcg taccggtccc cgagcgcccc
      841 catccgactt ttttcgcgca atttctcgtg caattgcacg cgagcgctct aagcgtggat
      901 aatagccgtg gcgtcaaccc cccggtcgac gccccgttct gacgtcgcat cagggagatg
      961 ctgtgccgtc acctgcgcat ccaacgctcc ggtcgcccgg cgaacccggg tcggaggcag
     1021 gcgaggcgac ccgctccgga ggactcccgt acccggtgcg ggccaccgcc ggccgggccg
     1081 tcccgccgtc gtccgccccg tacccgggca gccccgctct cggcggccgc ccgaccgccg
     1141 ccgtactgcg cgtcgcgtgc agcggggagg ggttcgcccg ggcccgggtc ttcacgcggg
     1201 acaccctgcg cgggtggtcg ctcgaccacc tcggcgacga cgcggtcctc gtgatcaccg
     1261 aactcgtctc caacgcgctg acgcacgcgg tgccgccgtc ggtggccggc gggccggaga
     1321 tcgggctcgg actcgccctg ggctccggcc ggctgaagct gaccgtctcc gatcccgggg
     1381 acgacgcgcc ccggctcaac ccgtccgacg gctccgcact ccgggagcac ggacgcggcc
     1441 tgtgcatcgt cgacgccctc gccgaggagt ggggctggac cccgcgcccg ccggcgggca
     1501 agacggtctg ggccacgttg tcgacccgcc ccctcacctg acccgactgc cgcggaaggg
     1561 ccccacacca tgcgcagcgc tccgacaccc gagccgagca tccggcgcac cgaccgtcag
     1621 acgccgcccg gctccccccg taccaccacc ctgctcgcgg gcctggacgg cgtgccctgg
     1681 agcgacatcc aggactccac gggctcggcg gcggccattc cgcggctgct gcgcaaggtc
     1741 gcccggggcg acgccgaaac cgcccgcgcc gctctcggcg acctgcgcag gcgcatctgc
     1801 cagtacggct tcgtcgtcga gcaggcgacc gccgcgaccg tgcccttcct gtgggagctg
     1861 gcgcagcggc cccaggtgag ctgccgggcg cagatcatcc agctgctcaa gaacatcgcc
     1921 gacgcccggc agtgggagac caccgccacc gcctacccca agctgctcaa ccaccgggag
     1981 aacccggtgg cctgggagcg cgcggcgcgg caggccgtcc gcgcccgacg ggacggactg
     2041 gagcggctgc tggcggacga cgacaccgag atcatgcgcg ccaccagcga actcgcccgc
     2101 accctcgggg actgagaccg gcaccgcctg gaccgggacc cgcgccctcc acgggggaga
     2161 aggcgcgggt ccctcccaag gcgcggaacg gtgtggcgtg ctcccggccg acagggtagg
     2221 aaagcgcggg aagcaagcgc ttgccccact cggtcaccag gaggaccacg ccgatggcgt
     2281 cgtcgatgga aaagccgctc gatcaccgct accggggcga acacccgata cgcacgctcg
     2341 tctacctgtt ccgcgccgac cgccgccgac tggccggcgc ggtcgccgtc ttcaccgtca
     2401 agcacagccc gatctggctg ctgcccctcg tcaccgccgc catcgtcgac accgtggtcc
     2461 agcacggtcc gatcaccgac ctgtggacca gcaccgggct catcatgttc atcctggtgg
     2521 tcaactaccc gctgcacctg ctctacgtcc gcctcctgta cggcagcgtg cgccgcatgg
     2581 gcaccgccct gcggtccgcg ctgtgcacgc gcatgcagca gctctccatc ggctaccact
     2641 cgcgggtcag cgcgggcgtg ctgcag
//