From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: E.coli enzyme III-Man function protein (manX (ptsL)) gene, complete
Date: 22 Dec 92 22:51:15 GMT
Distribution: bionet
Checksum: 64725 4

LOCUS       ECOMANXF     1474 bp ds-DNA             BCT       30-NOV-1992
DEFINITION  E.coli enzyme III-Man function protein (manX (ptsL)) gene, complete
            cds, and manY (pel) gene, 5' end.
ACCESSION   M36404 X17443
KEYWORDS    enzyme III-Man function protein; manX gene; manY gene; pel gene;
            ptsL gene.
SOURCE      E.coli (strain K12) DNA.
  ORGANISM  Escherichia coli
            Prokaryotae; Gram-negative facultatively anaerobic rods;
            Enterobacteriaceae.
REFERENCE   1  (bases 1 to 1474)
  AUTHORS   Saris,P.E., Liljestroem,P. and Palva,E.T.
  TITLE     Nucleotide sequence of manX (ptsL) encoding the enzyme III-Man
            (II-a-Man) function in the phosphotransferase system of Escherichia
            coli K-12
  JOURNAL   FEMS Microbiol. Lett. 49, 69-73 (1988)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     mRNA            120..>1474
                     /note="manXYZ operon mRNA (5' end put.); putative"
     CDS             258..1205
                     /note="enzyme III-Man function protein;  (manX (ptsL))"
                     /codon_start=1
                     /translation="MGWGCRAGCLKRQKVLLGEQENVGWINFVPGENAETLIEKYNAQ
                     LAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVERLMARDDDPS
                     FDELVALAVETGREGVKALKAKPVEKAAPAPGAAAPKAAPTPAKPMGPNDYMVIGLAR
                     IDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTAHVVDVAKM
                     IRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQVNNAVSVD
                     EKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     CDS             1268..>1474
                     /note="manY;  (pel) gene product"
                     /codon_start=1
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGYRSW
                     GYENRYYYRWYAGNDRAGLDEHRCC"
BASE COUNT      411 a    319 c    376 g    368 t
ORIGIN      
        1 cctttgcaaa cgaatgtgac aaggatattt tacctttcga aatttctgct aatcgaaagt
       61 taaattacgg atcttcatca cataaaataa ttttttcgat atctaaaata aatcgcgaaa
      121 cgcaggggtt tttggttgta gcccttatct gaatcgattc gattgtggac gacgattcaa
      181 aaatacatct ggcacgttga ggtgttaacg ataataaagg aggtagcaag tgaccattgc
      241 tattgttata ggcacacatg ggttggggct gcagagcagg ttgcttaaaa cggcagaaag
      301 tgctgttagg cgagcaggaa aacgtcggct ggatcaattt cgttccaggt gaaaatgccg
      361 aaacgctgat tgaaaagtac aacgctcagt tggcaaaact cgacaccact aaaggcgtgc
      421 tgtttctcgt tgatacatgg ggaggcagcc cgttcaatgc tgccagccgc attgtcgtcg
      481 acaaagagca ttatgaagtc attgcaggcg ttaacattcc aatgctcgtg gaaaggttaa
      541 tggcccgtga tgatgaccca agctttgatg aactggtggc actggcagta gaaacaggcc
      601 gtgaaggcgt gaaagcactg aaagccaaac cggttgaaaa agccgcgcca gcacccggtg
      661 ccgcagcacc aaaagcggct ccaactccgg caaaaccaat ggggccaaac gactacatgg
      721 ttattggcct tgcgcgtatc gacgaccgtc tgattcacgg tcaggtcgcc acccgctgga
      781 ccaaagaaac caatgtctcc cgtattattg ttgttagtga tgaagtggct gcggataccg
      841 ttcgtaagac actgctcacc caggttgcac ctccgggcgt aacagcacac gtagttgatg
      901 ttgccaaaat gattcgcgtc tacaacaacc cgaaatatgc tggcgaacgc gtaatgctgt
      961 tatttaccaa cccaacagat gtagagcgtc tcgttgaagg cggcgtgaaa atcacctctg
     1021 ttaacgtcgg tggtatggca ttccgtcagg gtaaaaccca ggtgaataac gcggtttcgg
     1081 ttgatgaaaa agatatcgag gcgttcaaga aactgaatgc gcgcggtatt gagctggaag
     1141 tccgtaaggt ttccaccgat ccgaaactga aaatgatgga tctgatcagc aaaatcgata
     1201 agtaacgtat tgtgttgatt atcactcagt tttcacactt aagtcttacg taaacaggag
     1261 aagtacaatg gagattacca ctcttcaaat tgtgctggta tttatcgtag cctgtatcgc
     1321 aggtatggga tcaatcctcg atgaatttca gtttcaccgt cctctaatcg cgtgtaccct
     1381 ggtgggctat cgttcttggg gatatgaaaa ccggtattat tatcggtggt acgctggaaa
     1441 tgatcgcgct gggctggatg aacatcggtg ctgc
//