From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Clostridium perfringens beta-galactosidase alpha-peptide (lacZ)
Date: 31 Dec 92 01:34:07 GMT
Distribution: bionet
Checksum: 28932 13

LOCUS       CLOCLSA      6568 bp ds-DNA             BCT       17-DEC-1992
DEFINITION  Clostridium perfringens beta-galactosidase alpha-peptide (lacZ)
            gene, partial cds; replication enzyme (rep) gene, 3' end cds;
            chloramphenicol acetyltransferase (catP) gene, complete cds.
ACCESSION   L02937
KEYWORDS    beta-galactosidase alpha-peptide; catP gene;
            chloramphenicol acetyltransferase; lacZ gene;
            multiple cloning site; rep gene; replication enzyme;
            replication origin.
SOURCE      Clostridium perfringens DNA.
  ORGANISM  Clostridium perfringens
            Prokaryotae; Firmicutes; Endospore-forming rods and cocci;
            Bacillaceae.
REFERENCE   1  (sites)
  AUTHORS   Garnier,T. and Cole,S.T.
  TITLE     Characterization of a bacteriocinogenic plasmid from Clostridium
            perfringens and molecular genetic analysis of the
            bacteriocin-encoding gene
  JOURNAL   J. Bacteriol. 168, 1189-1196 (1986)
  STANDARD  full automatic
REFERENCE   2  (sites)
  AUTHORS   Bannam,T.L. and Rood,J.I.
  JOURNAL   Unpublished (1991)
  STANDARD  full automatic
REFERENCE   3  (bases 1 to 6568)
  AUTHORS   Bannam,T.L. and Rood,J.I.
  TITLE     Clostridium perfringens - Escherichia coli shuttle vectors which
            carry single antibiotic resistance determinants
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
REFERENCE   4  (sites)
  AUTHORS   Sloan,J., Warner,T.A., Scott,P.T., Bannam,T.L., Berryman,D.I. and
            Rood,J.I.
  TITLE     Construction of a sequenced Clostridium perfringens-Escherichia
            coli shuttle plasmid
  JOURNAL   Antimicrob. Agents Chemother. 27, 207-219 (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     CDS             <310..>6555
                     /product="beta-galactosidase alpha-peptide"
                     /gene="lacZ"
                     /codon_start=1
                     /partial
                     /note="derived from pUC18; putative"
     misc_feature    1..57
                     /standard_name="multiple cloning site (MCS)"
                     /evidence="EXPERIMENTAL"
                     /note="derived from pUC19 (Genbank accession #X02514;
                     Yannisch-Perron et al. Gene 33, 103-119. 1985)"
     misc_feature    1190..1419
                     /function="C. perfringens pIP404 replication origin"
                     /standard_name="ori"
                     /evidence="EXPERIMENTAL"
                     /note="derived from complete sequence of pIP404 (Garnier
                     and Cole, plasmid 19,134-150.1988; GenBank accession
                     #M32882)"
     misc_feature    5990..6210
                     /function="replication region from pUC18"
                     /standard_name="oriEC"
                     /note="putative"
     CDS             complement(<1482..2702)
                     /function="pIP404 replication"
                     /gene="rep"
                     /note="derived from pIP404 sequence (Garnier and Cole,
                     plasmid 19, 134-150. 1988; GenBank accession #M32882;
                     putative"
                     /product="replication enzyme"
                     /codon_start=1
                     /translation="VRNALKLNKKNYIDNIHSRSKGWITRSVIDKKGYSQWHYKYAEL
                     KDLDMSDENIYITLNTFYKPCRRLENIKELNTLFIDLDYYKTGKTKDQVLMDLEKNYF
                     NQSIPIPNYVIDSGRGMYLIWIINAVPSKALPLWKAVQEYLYNQLKYFGADRQALDAT
                     RILRVPGSINSKSKTVVNILDEYEYIYDLREIQNGFLPELKPYERKKGRPSKINYIYR
                     ERSLYYGRIQDIIKLCELREYDLKGHRELILFLYRYYLCSFTEDIEKALNDVLELNSM
                     FRQHLSEREVIRATRSAERCYLDKNKQYKYKNETLIELLEITEEEQKYMTIIISKKEY
                     KRRENIRGKKNYQEQLKAKGKATKKEELNVLRKKIKALKEKGFKNKEITLMLEVPIKT
                     LERHITYMKKNGLL"
     CDS             complement(4207..4830)
                     /standard_name="CAT"
                     /gene="catP"
                     /evidence="EXPERIMENTAL"
                     /citation=[2]
                     /note="derived from sequence of C. perfringens catP gene
                     (T. Bannam and J. Rood unpublished results; GenBank
                     accession #M74769)"
                     /product="chloramphenicol acetyltransferase"
                     /codon_start=1
                     /translation="MVFEKIDKNSWNRKEYFDHYFASVPCTYSMTVKVDITQIKEKGM
                     KLYPAMLYYIAMIVNRHSEFRTAINQDGELGIYDEMIPSYTIFHNDTETFSSLWTECK
                     SDFKSFLADYESDTQRYGNNHRMEGKPNAPENIFNVSMIPWSTFDGFNLNLQKGYDYL
                     IPIFTMGKYYKEDNKIILPLAIQVHHAVCDGFHICRFVNELQELINS"
BASE COUNT     2004 a   1273 c   1017 g   2274 t
ORIGIN      
        1 gaattcgagc tcggtacccg gggatcctct agagtcgacc tgcaggcatg caagcttggc
       61 actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg
      121 ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg
      181 cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc ctgatgcggt attttctcct
      241 tacgcatctg tgcggtattt cacaccgcat atggtgcact ctcagtacaa tctgctctga
      301 tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc
      361 ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg
      421 tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg tgatacgcct
      481 atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg
      541 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
      601 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
      661 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
      721 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
      781 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
      841 acgttttcca atgatgagca cttttaaatt aaaaatgaag ttttaaaact tcatttttaa
      901 tttaaattaa aaatgaagtt ttatcaaaaa aatttccaat aatcccactc taagccacaa
      961 acacgcccta taaaatcccg ctttaatccc actttgagac acatgtaata ttactttacg
     1021 ccctagtata gtgataattt tttacattca atgccacgca aaaaaataaa ggggcactat
     1081 aataaaagtt ccttcggaac taactaaagt aaaaaattat ctttacaacc tccccaaaaa
     1141 aaagaacagg tacaaagtac cctataatac aagcgtaaaa aaatgagggt aaaaataaaa
     1201 aaataaaaaa ataaaaaaat aaaaaaataa aaaaaataaa aaaataaaaa aataaaaaaa
     1261 taaaaaaata aaaaaataaa aaaataaaaa aataaaaaaa tataaaaata aaaaaatata
     1321 aaaataaaaa aatataaaaa taaaaaaata taaaaataaa aaaataaaaa aatataaaaa
     1381 taaaaaaata aaaaaatata aaaatatttt ttatttaaag tttgaaaaaa atttttttat
     1441 attatataat ctttgaagaa aagaatataa aaaatgagcc tttataaaag cccatttttt
     1501 ttcatatacg taatatgacg ttctaatgtt tttattggta cttctaacat tagagtaatt
     1561 tctttatttt taaagccttt ttctttaagg gcttttattt tttttcttaa tacatttaat
     1621 tcctcttttt ttgttgcttt tcctttagct tttaattgct cttgataatt ttttttacct
     1681 ctaatatttt ctcttctctt atattccttt ttagaaatta ttattgtcat atatttttgt
     1741 tcttcttctg taatttctaa taactctata agagtttcat tcttatactt atattgctta
     1801 tttttatcta aataacatct ttcagcactt ctagttgctc ttataacttc tctttcactt
     1861 aaatgttgtc taaacatact attaagttct aaaacatcat ttaatgcctt ctcaatgtct
     1921 tctgtaaagc tacaaagata atatctatat aaaaataata taagctctct gtgtcctttt
     1981 aaatcatatt ctcttagttc acaaagtttt attatgtctt gtattcttcc ataatataaa
     2041 cttctttctc tataaatata atttattttg cttggtctac cctttttcct ttcatatggt
     2101 tttaattcag gtaaaaatcc attttgtatt tctcttaagt cataaatata ttcgtactca
     2161 tctaatatat tgactactgt ttttgattta gagtttatac ttcctggaac tcttaatatt
     2221 ctggttgcat ctaaggcttg tctatctgct ccaaagtatt ttaattgatt atataaatat
     2281 tcttgaaccg ctttccataa tggtaatgct ttactaggta ctgcatttat tatccatatt
     2341 aaatacattc ctcttccact atctattaca tagtttggta taggaatact ttgattaaaa
     2401 taattctttt ctaagtccat taatacctgg tctttagttt tgccagtttt ataataatcc
     2461 aagtctataa acagtgtatt taactctttt atattttcta atcgcctaca cggcttataa
     2521 aaggtattta gagttatata gatattttca tcactcatat ctaaatcttt taattcagcg
     2581 tatttatagt gccattggct atatcctttt ttatctataa cgctcctggt tatccaccct
     2641 ttacttctac tatgaatatt atctatatag ttctttttat tcagctttaa tgcgtttctc
     2701 acttattcac ctccccttct gtaaaactaa gaaaattata tcatattttc aataattatt
     2761 aactattctt aaactcttaa taaaaaatag agtaagtccc caattgaaac ttaatctatt
     2821 ttttatgttt taatttatta tttttattaa aatattttaa actaaattaa atgattcttt
     2881 ttaatttttt actatttcat tccataatat attactataa ttatttacaa ataatatttc
     2941 ttcatttgta atatttagat gatttactaa ttttagtttt tatatattaa ataattaatg
     3001 tataatttat ataaaaaatc aaaggagctt ataaattatg attatttcca aagatactaa
     3061 agatttaatt ttttcaattt taacaatact ttttgtaata ttatgtttaa atttaattgt
     3121 atttttttca tataataaag ccgttgaagt aaaccaatcc attttcctta tgatgttatt
     3181 attaaattta agttttataa taatatcttt attatattta ttgtttttaa aaaaactagt
     3241 gaaatttccg gctttattaa acttattttt aggaatttta ttttcatttt catctttaca
     3301 ggatttgatt atatctttaa atatgtttta tcaaatatta tctttttcta aatttatata
     3361 tatttttatt atatttatta ttatatatat tttattttta agtttctttc taacagctat
     3421 taaaaagaaa cttaaaaata aaaacacgta ctctaaacca ataaataaaa ctatttttat
     3481 tattgctgcc ttgattggaa tagtttttag taaaattaat ttcaatattc cacaatatta
     3541 tattataagc tagctttgca ttgtactttt caatcgcttc acgaatgcgg ttatctccga
     3601 aagataaagt cttttcatct tccttgatga agataagatt ttctccgtct ccgccggcag
     3661 aattgaagcg gggtactacg gtatcgtctg cgtcatcttc cgttgtctga tagatgatag
     3721 tcataggctc attttcttcc gtttcggtaa aggggatagg ttcgcccttt gagagcaggg
     3781 cggcgatgga aagcattaac ttgcttttcc catcgcccgg atctccctgc aatagcgtaa
     3841 ctttgccaaa cggaatatac ggataccaca gccactttac ttctttcggc tcgatttcac
     3901 ttgccttgat gatttcaaga ggtacgctga aattcatttc gttttcattt agtttcattt
     3961 tttcttgttc tccttttctc tgaaaatata aaaaccacag attgatacta aaaccttggt
     4021 tgtgttgctt ttcggggctt aaatcaagga aaaatccttg ttttaagcct ttcaaaaaga
     4081 aacacaaggt ctttgtacta acctgtggtt atgtataaaa ttgtagattt tagggtaaca
     4141 aaaaacaccg tatttctacg atgtttttgc ttaaatactt gtttttagtt acagacaaac
     4201 ctgaagttaa ctatttatca attcctgcaa ttcgtttaca aaacggcaaa tgtgaaatcc
     4261 gtcacatact gcgtgatgaa cttgaattgc caaaggaagt ataattttgt tatcttcttt
     4321 ataatatttc cccatagtaa aaataggaat caaataatca tatcctttct gcaaattcag
     4381 attaaagcca tcgaaggttg accacggtat catagataca ttaaaaatgt tttccggagc
     4441 atttggcttt ccttccattc tatgattgtt tccataccgt tgcgtatcac tttcataatc
     4501 tgctaaaaat gatttaaagt cagacttaca ctcagtccaa aggctggaaa atgtttcagt
     4561 atcattgtga aatattgtat agcttggtat catctcatca tatatcccca attcaccatc
     4621 ttgattgatt gccgtcctaa actctgaatg gcggtttaca atcattgcaa tataataaag
     4681 cattgcagga tatagtttca ttcccttttc ctttatttgt gtgatatcca ctttaacggt
     4741 catgctgtat gtacaaggta cacttgcaaa gtagtggtca aaatactctt ttctgttcca
     4801 actattttta tcaatttttt caaataccat ctaagttccc tctcaaattc aagtttatcg
     4861 ctctaatgaa caaagatatt ataccacatt tttgtgaatt tttcaacttg cccacttcga
     4921 ctgcactccc gacttaataa cttcttgaac acttgccgaa aaagaaaaac tgccgggtac
     4981 gtacccggga tcgatccccg ccgagcgctt agtgggaatt tgtacccctt atcgatacaa
     5041 attccccgta ggcgctaggg acactttttc actcgttaaa aagttttgag aatattttat
     5101 atttttgttc atgtaatcac tccttcttaa ttacaaattt ttagcatcta atttaacttc
     5161 aattcctatt atacaaaatt ttaagatact gcactatcaa cacactctta agtttgcttc
     5221 taagtcttat ttccataact tcttttacgt ttccgggtac aattcgtaat catgtcatag
     5281 ctgtttcctg tgtgaaattc ttatccgctc acaattccac acaacatacg agccggaagc
     5341 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     5401 tcactgcccg ctttccagtc gggaaacctg tcgtgccaga aaacttcatt tttaatttaa
     5461 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     5521 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     5581 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     5641 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     5701 gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     5761 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     5821 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     5881 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     5941 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     6001 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     6061 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     6121 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     6181 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     6241 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     6301 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc
     6361 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa
     6421 agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc
     6481 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca
     6541 cacaggaaac agctatgacc atgattac
//