From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Enterobacter aerogenes alpha-acetolactase decarboxylase (budA)
Date: 21 Dec 92 20:41:20 GMT
Distribution: bionet
Checksum: 05479 4

LOCUS       ENTACETDEC   1064 bp ds-DNA             BCT       20-NOV-1992
DEFINITION  Enterobacter aerogenes alpha-acetolactase decarboxylase (budA)
            gene, complete cds.
ACCESSION   L04506
KEYWORDS    alpha-acetolactate decarboxylase; budA gene.
SOURCE      Enterobacter aerogenes (library: VTT-E-87292) DNA.
  ORGANISM  Enterobacter aerogenes
            Prokaryotae; Gram-negative facultatively anaerobic rods;
            Enterobacteriaceae.
REFERENCE   1  (bases 1 to 1064)
  AUTHORS   Blomqvist,K., Nikkola,M., Lehtovaara,P., Suihko,M.-L.,
            Airaksinen,U., Straby,K.B., Knowles,J.K. and Penttilae,M.E.
  TITLE     Characterization of the genes of the 2.3-Butanediol operon from
            Klebsiella terrigena and Enterobacter aerogenes
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     mRNA            143..>954
                     /note="alternate"
                     /gene="budA"
     mRNA            145..>954
                     /note="alternate"
                     /gene="budA"
     RBS             165..169
                     /gene="budA"
     CDS             175..954
                     /EC_number="4.1.1.5"
                     /gene="budA"
                     /product="alpha-acetolactate decarboxylase"
                     /codon_start=1
                     /translation="MMMHSSACDCEASLCETLRGFSAQHPDSVIYQTSLMSALLSGVY
                     VGETTIADLLAHGDFGLGTFNELDGEMIAFSSQVYQLRADGSARAAKPEQKTPFAVMT
                     WFQPQYRKTFNGPVSRQQIHDVIDQQIPSDNLFCVRIDGNFRHAHTRTVPRQTPPYRA
                     MTDVLDDQPVFRFNQREGVLVGFRTPQHMQGINVAGYHEHFITDDRQGGGHLLDYQLE
                     SGVLTFGEIHKLMIDLPADSAFLQANLHPSNLDAAIRAVEN"
BASE COUNT      239 a    315 c    303 g    207 t
ORIGIN      
        1 cgctttggcc gccctggtga agtgtcgctc gcgtgcaacc gcgacaaaat agcgcagata
       61 acgaagttcc atatcaaaaa cgtctcaaac cagcatggat tctatattgg aactctctgc
      121 tgaatcgggt caacatttat taaaccttta taaataaagt tgaagaggac gggcatgatg
      181 atgcattcat ctgcatgcga ctgtgaggcg agtttatgcg agaccctgcg tgggttctcg
      241 gctcagcatc ctgacagcgt gatctatcag acatcgctaa tgagcgccct gctaagcggt
      301 gtctacgtag gggagacgac gatcgccgac ctgctggcac acggtgattt tggtctcggc
      361 acctttaacg agctggacgg tgaaatgatt gccttcagca gccaggtgta ccagctgcgc
      421 gccgacggca gcgcccgcgc cgcgaagccg gagcaaaaaa cgccgttcgc ggtgatgacc
      481 tggttccagc cgcagtaccg caaaaccttt aacggtccgg tcagccgtca gcagatccac
      541 gacgtgatcg accagcagat cccctccgat aacctgttct gcgtgcgcat cgatggcaac
      601 ttccgccatg cgcatacccg caccgttccg cgccagacgc cgccgtaccg ggcaatgacc
      661 gacgtgctgg acgaccagcc ggtgttccgc tttaaccaac gcgagggcgt gctggtcggg
      721 ttccgcaccc cgcagcatat gcagggcatc aacgtggcgg gctatcacga gcactttatc
      781 accgacgacc gtcagggcgg cggccatctg ctcgactatc agctggagag cggcgtgctc
      841 acctttggcg aaatccacaa gctgatgatt gacctgcccg ccgacagcgc gtttttacag
      901 gccaacctgc accccagcaa ccttgatgcg gctatccgcg ccgtcgaaaa ctaacaggag
      961 aactaccgtg aacagtgaga aacagtcacg tcagtgggcg cacggcgccg atatggttgt
     1021 cggccagctg gaagcgcagg gtgtgaagca ggtgttcggg atcc
//