From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Erysipelothrix rhusiopathiae protein associated with DNA synthesis
Date: 18 Aug 92 10:00:51 GMT
Distribution: bionet
Checksum: 17333     5

LOCUS       ERYDNAK      2052 bp ds-DNA             BCT       18-AUG-1992
DEFINITION  Erysipelothrix rhusiopathiae protein associated with DNA synthesis
            (dnaK) gene, complete cds.
ACCESSION   M98865
KEYWORDS    ATPase; HSP70; dnaK gene; heat shock protein 70.
SOURCE      Erysipelothrix rhusiopathiae (strain E1-6P) DNA.
  ORGANISM  Erysipelothrix rhusiopathiae
            Prokaryotae; Firmicutes; Regular asporogenous rods.
REFERENCE   1  (bases 1 to 2052)
  AUTHORS   Partridge,J., King,J. and Blum,P.
  TITLE     Cloning, heterologous expression and characterization of the
            Erysipelothrix rhusiopathiae DnaK protein
  JOURNAL   Unpublished (1992)
  STANDARD  full staff_entry
FEATURES             Location/Qualifiers
     RBS             69..72
                     /gene="dnaK"
     CDS             83..1885
                     /function="ATPase"
                     /standard_name="HSP70"
                     /product="DnaK protein"
                     /gene="dnaK"
                     /evidence=EXPERIMENTAL
                     /codon_start=1
                     /translation="MSKVIGIDLGTTNSAVSVMDGGEAKVITNPEGNRTTPSVVSFKN
                     GERIVGDAAKRQVVTNPNSAVSVKRLIGTGEKVTLEGKDYTPEEISAMILGYMKSYAE
                     DYLGEKVTKAVITVPAYFNDAQRQATKDAGKIAGLEVERIINEPTAAALAFGIDKTDK
                     EEKVLVFDLGGGTFDVSILELADGTFEVLSTAGDNKLGGDDFDNIVVDYLVDIFKKEN
                     GIDLSSDKMAMQRLKEAAEKAKKDLSSTVNASISLPFISAGENGPLHLETTLSRAKFE
                     EMTKSLVERTMVPVRQALKDAGLTKNDIHQVLLVGGSTRIPAVVEAVKNDLGKEPNKS
                     VNPDEVVAMGAAIQGGVISGDGKDVLLLDVTPLSLGIETMGGVMTVLIERNTTIPTSK
                     SQVFSTAADNQPAVDINVLQGERPMAKDNKSLGLFKLDGIAPAKRGIPQIEVTFDIDV
                     NGIVNVSAMDKGTNKKQSITISNSSGLSDEEIERMVREAEENASEDLRLKEEAELKNR
                     AEQFIHQIDESLASEDSPVDDAQKEEVTKLRDELQAAMDNNDFETLKEKLDQLEQAAQ
                     AMSQAMYEQQAGQAEVDASSSDETVVDAEFEEKN"
                     /citation=[1]
BASE COUNT      764 a    306 c    422 g    560 t
ORIGIN
        1 gaattccaaa aaggatatat gttaaaagat cgcattctac gtgcgagttt agtaaaagtg
       61 agtcaatagg aggatatata ttatgagtaa agttattggt attgatttag gaacaacaaa
      121 ctcagctgtt tccgtaatgg acggtggaga agcaaaagta attacaaacc cagaaggaaa
      181 tcgtacaacg ccttctgttg taagttttaa aaatggtgaa cgtattgttg gggatgctgc
      241 aaagcgtcaa gttgttacaa accctaactc agcagtatct gttaaacgtt taattggtac
      301 aggcgaaaaa gttacacttg aaggcaaaga ttatacacca gaagaaattt cagcaatgat
      361 cttaggttat atgaagagct atgcagaaga ttacctcggt gaaaaagtta caaaagctgt
      421 aatcacagtt cctgcatact ttaatgatgc acaacgtcaa gctacaaaag atgctggtaa
      481 gattgctgga ttagaagtag aacgtattat taacgaacca actgcagctg cgcttgcatt
      541 tggaattgat aagacagata aggaagaaaa agttcttgta tttgaccttg gtggtggtac
      601 atttgacgtt tcgattcttg aattagcaga tggtactttt gaagtattat caacagctgg
      661 tgacaacaaa ttaggtggag atgattttga caacatcgtt gttgattatt tagtagatat
      721 tttcaaaaaa gagaacggaa ttgatttatc atccgacaag atggcaatgc aacgtctaaa
      781 agaagcagca gaaaaagcga aaaaagattt atcttcaact gtaaatgctt caatttcatt
      841 accatttatc tcagcaggtg aaaatggtcc attacacttg gaaacaacat tatcacgtgc
      901 taaatttgaa gaaatgacaa agagccttgt tgaacgtaca atggttccag ttcgtcaagc
      961 attaaaagat gctggactta caaaaaatga tattcatcaa gtattacttg ttggtggatc
     1021 aacacgtatt cctgcagttg ttgaagcagt taaaaatgat ttaggaaaag aacctaataa
     1081 atctgtaaac cctgatgaag ttgttgcaat gggtgccgca attcaaggtg gtgttatttc
     1141 tggagatggt aaagatgtat tgcttcttga cgttacacca ttatcattag gtattgaaac
     1201 aatgggtggt gtgatgacag ttcttattga acgtaataca acaatcccaa catcaaaatc
     1261 acaagtattc tcaacagcag cagataatca accagctgta gatattaacg tattacaagg
     1321 tgaacgtcca atggctaaag acaataaatc acttggttta tttaaattag atggtattgc
     1381 acctgcaaaa cgtggtattc ctcaaattga agttacattc gatattgatg taaatggtat
     1441 cgtaaacgtt tcagcaatgg ataaaggaac aaacaaaaaa caatctatta caatttcaaa
     1501 cagttcagga ttaagtgatg aagaaattga acgtatggtt cgtgaagcgg aagaaaatgc
     1561 ttcagaagat ttacgtttaa aagaagaagc agaacttaaa aaccgtgcag aacaattcat
     1621 ccatcaaatc gatgaatcat tagcaagtga agattcacct gtggatgatg ctcaaaaaga
     1681 agaagttaca aaattacgtg atgaattgca agcagcaatg gacaacaatg attttgaaac
     1741 attaaaagaa aaacttgatc aattagaaca agcagctcaa gcaatgtcac aagcaatgta
     1801 tgaacaacaa gcaggccaag ctgaagtaga tgcttcgtca agtgatgaaa cagttgttga
     1861 cgctgaattt gaagaaaaaa actagtttaa aaactttcgg aggtagatat ggcagataaa
     1921 agagattttt atgaaatact tggagtaagt aaatctgcca cagatgcaga aattaagaaa
     1981 gcctatcgcc agttagcgaa gaagtaccat cctgatatta acaaagagga tggtgctgaa
     2041 gctaaattta aa
//