From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Trypanosoma brucei (clone WaTat1.1, g2.00kb DraI fragment) surface
Date: 24 Jul 92 10:10:34 GMT
Distribution: bionet
Checksum: 56800     4

LOCUS       TRBSURGLYF   1240 bp ds-DNA             INV       24-JUL-1992
DEFINITION  Trypanosoma brucei (clone WaTat1.1, g2.00kb DraI fragment) surface
            glycoprotein (VSG) gene, 5'end.
ACCESSION   M83699
KEYWORDS    surface glycoprotein.
SOURCE      Trypanosoma brucei DNA.
  ORGANISM  Trypanosoma brucei
            Eukaryota; Animalia; Protozoa; Sarcomastigophora; Mastigophora;
            Kinetoplastida; Trypanosomatina; Trypanosomatidae.
REFERENCE   1  (bases 1 to 1240)
  AUTHORS   Barbet,A.F., Davis,W.C. and McGuire,T.C.
  TITLE     Cross-neutralization of two different trypanosome populations
            derived from a single organism
  JOURNAL   Nature 300, 453-456 (1982)
  STANDARD  full staff_review
REFERENCE   2  (bases 1 to 1240)
  AUTHORS   Barbet,A.F., Myler,P.J., Williams,R.O. and McGuire,T.C.
  TITLE     Shared surface epitopes among trypanosomes of the same serodeme
            expressing different variable surface glycoprotein genes
  JOURNAL   Mol. Biochem. Parasitol. 32, 191-200 (1989)
  STANDARD  full staff_review
REFERENCE   3  (bases 1 to 1240)
  AUTHORS   Kamper,S.M. and Barbet,A.F.
  TITLE     Surface epitope variation via mosaic gene formation is potential
            key to long-term survival of Trypanosoma brucei.
  JOURNAL   Mol. Biochem. Parasitol. 53, 33-44 (1992)
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     CDS             1..1240
                     /standard_name="VSG"
                     /product="surface glycoprotein"
                     /gene="VSG"
                     /evidence=EXPERIMENTAL
                     /codon_start=1
                     /translation="MTKSPTKLSQVALSYLLLIAAAPSHVQPASQSALDKGLWQGICK
                     ATAELNKVPGTMTHELTQILQKAKTMRRGELKAQIFGLRHAGTPSANKAAVMAAYFSR
                     RYKQIIEALETQDIEKAIDAAAKAAYLQGRIDDPLHLLAAVDNTNNLCLSTTGATGNK
                     PAKTPGKLGDVDCPLSAPSAKNKAAELSGITQAGFTMLKKDVSSGDAKQVASGTKKCN
                     LLSSVNGQGFGQTEAAISTHHEILGGYITIKDTNTEIDVTAHNKAHTVESGKHESWTA
                     AHAAIMVSPKHTTTPYINETGEIHSRSDMTKVLQAVYSAKPPISNTGLENKVIEIFQG
                     KEAKKLQDYEDAVNQVTIPAGIAGLETDQTLAAVNDEDKLIAIRAFYEQQVGAAYGKM
                     VATISEASNKQTATAPADCGK"
                     /citation=[2]
                     /citation=[1]
                     /citation=[3]
                     /partial
BASE COUNT      440 a    316 c    303 g    181 t
ORIGIN
        1 atgacaaaaa gtccaacgaa actgagccaa gtagcgcttt catacttgtt actaatcgcc
       61 gcagcaccga gtcatgtaca acccgctagt caatcagcct tggacaaagg gctctggcag
      121 ggcatatgca aagcgacggc agagctgaac aaagttcccg ggacgatgac gcacgaattg
      181 acgcagatcc tccaaaaagc gaagacgatg cggcgtggag aactgaaagc acaaatattt
      241 gggctaaggc atgcaggaac accatcggca aacaaagcag cggtaatggc ggcgtatttc
      301 agccgtaggt acaagcagat aattgaagcg cttgagacgc aagacatcga aaaagccatc
      361 gacgcggcag ccaaagcagc gtaccttcaa ggcagaatag acgacccgct tcatttactg
      421 gcagctgtgg ataacacaaa caatctttgt ctgtctacga ccggagcgac aggcaacaaa
      481 ccggcaaaga cgcccggcaa actgggcgac gtcgactgcc cactatcagc gccgtcagca
      541 aaaaacaagg cagctgaatt aagcggtata acacaagccg gctttacgat gctaaaaaag
      601 gatgtgtcaa gcggcgacgc caaacaagtc gcgagtggca caaagaaatg taaccttcta
      661 agttcagtaa acggccaagg cttcggacag acagaggcgg cgatcagcac ccaccacgaa
      721 atactcggag gatacattac aatcaaagac acaaacactg aaatagacgt aaccgcgcac
      781 aacaaagcac acacagtcga aagcggcaag cacgaaagct ggacggcggc acatgcagcc
      841 attatggtga gcccaaaaca cacaacgaca ccctatatca atgaaactgg ggaaattcac
      901 agcaggtcag acatgacaaa agtgttgcaa gccgtttaca gcgcaaaacc gccgataagc
      961 aacactggcc tagagaacaa ggtcatagaa atatttcaag gaaaagaagc aaagaaactg
     1021 caagactacg aagacgctgt aaaccaagta acgataccag ccggcatagc aggactggaa
     1081 actgaccaga cgctagcagc agtaaacgac gaagacaaac taatcgcaat cagagccttt
     1141 tatgaacagc aggtcggcgc agcctatggc aaaatggtag cgacgatatc cgaagcaagc
     1201 aataaacaaa cagccaccgc cccagcagac tgcggtaaaa
//