From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Z.ramigera thiolase gene, complete cds.
Date: 15 Sep 92 10:06:34 GMT
Distribution: bionet
Checksum: 58650     4

LOCUS       ZOGTHIZR     1524 bp ds-DNA             BCT       15-SEP-1992
DEFINITION  Z.ramigera thiolase gene, complete cds.
ACCESSION   J02631
KEYWORDS    thiolase.
SOURCE      Z.ramigera (strain I-16-M (ATCC 19623)) DNA, clones LDBK[1,2].
  ORGANISM  Zoogloea ramigera
            Prokaryotae; Gracilicutes; Scotobacteria; Pseudomonadaceae.
REFERENCE   1  (bases 1 to 1524)
  AUTHORS   Peoples,O.P., Masamune,S., Walsh,C.T. and Sinskey,A.J.
  TITLE     Biosynthetic thiolase from Zoogloea ramigera: III. Isolation and
            characterization of the structural gene
  JOURNAL   J. Biol. Chem. 262, 97-102 (1987)
  STANDARD  full staff_review
REFERENCE   2  (sites)
  AUTHORS   Williams,S.F., Palmer,M.A., Peoples,O.P., Walsh,C.T., Sinskey,A.J.
            and Masamune,S.
  TITLE     Biosynthetic thiolase from Zoogloea ramigera: Mutagenesis of the
            putative active-site base CYS-378 to SER-378 changes the
            partitioning of the acetyl S-enzyme intermediate
  JOURNAL   J. Biol. Chem. 267, 16041-16043 (1992)
  STANDARD  full staff_review
COMMENT     A potential ribosome binding site is located at positions 280-283.
FEATURES             Location/Qualifiers
     CDS             291..1466
                     /note="thiolase (EC 2.3.1.9)"
                     /codon_start=1
                     /translation="MSTPSIVIASARTAVGSFNGAFANTPAHELGATVISAVLERAGV
                     AAGEVNEVILGQVLPAGEGQNPARQAAMKAGVPQEATAWGMNQLCGSGLRAVALGMQQ
                     IATGDASIIVAGGMESMSMAPHCAHLAGVKMGDFKMIDTMIKDGLTDAFYGYHMGTTA
                     ENVAKQWQLSRDEQDAFAVASQNKAEAAQKDGRFKDEIVPFIVKGRKGDITVDADEYI
                     RHGATLDSMAKLRPAFDKEGTVTAGNASGLNDGAAAALLMSEAEASRRGIQPLGRIVS
                     WATVGVDPKVMGTGPIPASRKALERAGWKIGDLDLVEANEAFAAQACAVNKDLGWDPS
                     IVNVNGGAIAIGHPIGASGARILNTLLFEMKRRGARKGLATLCIGGGMGVAMCIESL"
BASE COUNT      264 a    523 c    484 g    253 t
ORIGIN      1 bp upstream of EcoRI site.
        1 gaattcctca tccccgtgtt cctcttttct tttttgagcc ttgcgagtat ccgcaaaaag
       61 acgcttcctg acaatcgaat tgtgcgatgc ggcgagggat gctgcgcaac atggattttc
      121 atgctgcgcg gccccccgca tgaaatttta gcgccgcggg atatcccgtt tgactctcct
      181 tctccgcttt gccagttttg atttcgggag aaccgccggg ctcgcgccgt tcgtgcgccg
      241 gacccggttc caaggccggc cgcaaggctg ccagaactga ggaagcacat atgagcaccc
      301 cgtccatcgt catcgccagc gcccgcaccg cggtcggttc cttcaacggc gctttcgcca
      361 acacgcccgc ccatgaactc ggggcgaccg tgatttcggc ggttctcgag cgcgcgggcg
      421 ttgcggcggg cgaggtgaac gaggtgattc tcggccaggt gctgccggcc ggcgaaggcc
      481 agaacccggc ccgccaggcc gccatgaagg ccggcgtgcc gcaggaggcg accgcctggg
      541 gcatgaacca gctttgcggc tcgggcctgc gcgccgtcgc gctcggcatg cagcagatcg
      601 ccacgggcga tgcgagcatc atcgtcgccg gcggcatgga atccatgtcc atggccccgc
      661 attgcgcgca tctggccggc gtgaagatgg gcgatttcaa gatgatcgac acgatgatca
      721 aggacggcct gaccgacgcc ttctacggct accacatggg cacgaccgcc gagaatgtcg
      781 ccaagcagtg gcagctttcc cgcgacgagc aggacgcctt cgccgtcgcc tcgcagaaca
      841 aggccgaggc cgcccagaag gacggccgct tcaaggacga gatcgttccc ttcatcgtca
      901 agggccgcaa gggcgacatc acggtcgatg ccgacgaata tatccgccac ggcgcgacgc
      961 tcgattccat ggcgaagctc cgcccggcct tcgacaagga aggcacggtg acggccggca
     1021 acgcctccgg cctcaatgac ggcgcggccg cggccctcct gatgagcgaa gcggaagcct
     1081 cgcgccgcgg catccagccg ctcggccgca tcgtttcctg ggcgacggtc ggcgtcgatc
     1141 ccaaggtcat gggcaccggc ccgatcccgg cctcccgcaa ggcgctcgag cgcgccggct
     1201 ggaagatcgg cgatctcgac ctcgtggaag ccaacgaagc cttcgcggcg caggcctgcg
     1261 cggtcaacaa ggacctcggc tgggatccgt ccatcgtcaa cgtcaacggc ggtgccatcg
     1321 ccatcggcca cccgatcggc gcgtccggcg cccgcatcct caacacgctc ctcttcgaga
     1381 tgaagcgtcg cggcgcccgc aagggtctcg ccacgctctg catcggcggc ggcatgggcg
     1441 tggcgatgtg catcgagagc ctttaggcgt cagcttatcc aaaactttgc catgacctgc
     1501 cgccggcgag ggcgacaggt cgac
//