From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: S. cerevisiae OCH1 gene for mannosyltransferase, complete cds.
Date: 29 Jul 92 10:03:59 GMT
Distribution: bionet
Checksum: 47870     6

LOCUS       YSCOCH1      2578 bp ds-DNA             PLN       29-JUL-1992
DEFINITION  S. cerevisiae OCH1 gene for mannosyltransferase, complete cds.
ACCESSION   D11095
KEYWORDS    OCH1 protein; mannosyltransferase; outer chain elongation;
            protein glycosylation.
SOURCE      Saccharomyces cerevisiae (strain EG1-103) DNA.
  ORGANISM  Saccharomyces cerevisiae
            Eukaryota; Fungi; Eumycota; Hemiascomycetes; Endomycetales;
            Saccharomycetaceae.
REFERENCE   1  (bases 1 to 2578)
  AUTHORS   Nakayama,K.-., Nagasu,T., Shimma,Y.-., Kuromitsu,J.-. and Jigami,Y.
  TITLE     OCH1 encodes a novel membrane bound mannosyltransferase: Outer
            chain elongation of asparagine-linked oligosaccharides
  JOURNAL   EMBO J. 11, 2511-2519 (1992)
  STANDARD  full staff_review
COMMENT     Submitted (08-MAY-1992) to DDBJ by:
            Ken-ichi Nakayama
            National Chemical Laboratory for Industry
            1-1 Higashi, Tsukuba
            Ibaraki 305
            Japan
            Phone:  0298-54-4678
            Fax:    0298-55-1397
FEATURES             Location/Qualifiers
     CDS             787..2229
                     /product="mannosyltransferase"
                     /gene="CHO1"
                     /codon_start=1
                     /translation="MSRKLSHLIATRKSKTIVVTVLLIYSLLTFHLSNKRLLSQFYPS
                     KDDFKQTLLPTTSHSQDINLKKQITVNKKKNQLHNLRDQLSFAFPYDSQAPIPQRVWQ
                     TWKVGADDKNFPSSFRTYQKTWSGSYSPDYQYSLISDDSIIPFLENLYAPVPIVIQAF
                     KLMPGNILKADFLRYLLLFARGGIYSDMDTMLLKPIDSWPSQNKSWLNNIIDLNKPIP
                     YKNSKPSLLSSDEISHQPGLVIGIEADPDRDDWSEWYARRIQFCQWTIQAKPGHPILR
                     ELILNITATTLASVQNPGVPVSEMIDPRFEEDYNVNYRHKRRHDETYKHSELKNNKNV
                     DGSDIMNWTGPGIFSDIIFEYMNNVLRYNSDILLINPNLNKNDEEGSESATTPAKDVD
                     NDTLSKSTRKFYKKISESLQSSNSMPWEFFSFLKEPVIVDDVMVLPITSFSPDVGQMG
                     AQSSDDKMAFVKHMFSGSWKEDADKNAGHK"
BASE COUNT      814 a    532 c    474 g    758 t
ORIGIN
        1 gtcgacaagg gtttcttgac tgaccgtgaa tactactccg ccttccacga catctggaac
       61 ttccataaga ccgacatgaa caagtactta gaaaagcatc atacagacga ggtttacatt
      121 gtcggtgtag ctttggagta ttgtgtcaaa gccaccgcca tttccgctgc agaactaggt
      181 tataagacca ctgtcctgct ggattacaca agacccatca gcgatgatcc cgaagtcatc
      241 aataaggtta aggaagagtt gaaggcccac aacatcaatg tcgtggataa ataagagctg
      301 aataatactt cttcaacctg atgaactagg gtggcttgca aatgcacaaa tctatataac
      361 aatatctata tatatgtatg tacaccgaat tccgacatat ggagaaggaa taataaaata
      421 ttaactaacg tctttacgcc tctctttatt cttttttggg taaattgctt aaactatttg
      481 gccggcccac cgcgaaaaga tttggctggg cctcaactaa acgcgccttt ttggactttt
      541 cacgttgcag ggacagcaac gtcaaaactt ctgcattaag gtagtttggt agcttggtag
      601 ccactttagt atttctgcct tcttcgaata ccgacattat ttctcgccaa tccacattct
      661 ctctccccat ctgcatcctt ttatatttaa tagggatagg ttgttttagt tctttgattc
      721 cgttttcatt tcaagagcaa taatagcaat ttggaaaaag aaagcaagta aaagaaagaa
      781 gagatcatgt ctaggaagtt gtcccacctg atcgctacaa ggaaatcaaa aacaatagtc
      841 gtaaccgtac ttcttattta ttctttgttg acatttcact tgtcaaacaa aaggctgctt
      901 tctcagtttt accctagcaa agatgatttc aagcaaactc ttctccctac gacttctcat
      961 tcacaagata taaatttgaa gaaacaaatt acagttaaca agaaaaaaaa tcaattgcat
     1021 aatttacgtg atcaattatc gtttgcgttt ccctacgact ctcaggcccc catcccgcaa
     1081 agggtgtggc agacctggaa agtcggcgca gatgataaga attttccctc ttcgttcaga
     1141 acctatcaaa aaacatggtc tggttcgtat tcaccggatt accaatattc tctgatttcg
     1201 gatgattcta ttatcccttt tttggagaat ctttacgcac ccgttccgat agtcatccaa
     1261 gcgtttaaat tgatgcctgg aaatatccta aaggcagatt ttttaaggta cctattatta
     1321 tttgcaagag gtggtattta ctcagatatg gatactatgc ttttgaagcc aattgattca
     1381 tggccttctc agaataagtc atggctaaac aacataatag atttgaataa acctattcct
     1441 tataagaact caaagccctc acttctctca agtgatgaga tatcacacca gccaggtttg
     1501 gtcatcggca ttgaggcaga tccggacaga gatgattgga gtgaatggta tgctcgtagg
     1561 atccagtttt gtcaatggac tatccaagcc aaaccaggtc acccaattct aagagaattg
     1621 atcttaaata ttactgcaac gactttggcg agcgtacaaa acccaggagt tcctgtcagt
     1681 gaaatgattg atccaagatt tgaagaagac tacaacgtaa actataggca caaaagacgt
     1741 catgatgaga cctacaaaca ctccgaattg aaaaataaca aaaatgttga tggctcagat
     1801 ataatgaatt ggacgggtcc aggtattttc tccgacatta ttttcgaata catgaacaat
     1861 gtgctccgat acaatagcga tatcttgtta atcaacccaa acctaaacaa gaacgacgaa
     1921 gaaggtagtg agagtgccac cacaccagca aaagatgttg ataacgatac gctgtccaaa
     1981 tcaacaagaa aattttataa gaaaatatca gagtctttgc aatcgtcaaa ttcaatgccc
     2041 tgggaattct tctctttttt gaaagaacca gttatcgttg atgatgtgat ggttctacca
     2101 ataacaagtt tttcaccaga tgtggggcag atgggcgccc agtctagtga cgacaaaatg
     2161 gcttttgtaa agcacatgtt tagcgggagc tggaaagaag atgctgataa aaatgcaggt
     2221 cataaataac gaggctctcc tttcatcata ccctctctaa ataaaattta ttcctagtta
     2281 ttcctttctt cttcatatcc ttaatcatgc attcactgcc atgtgcaaat aaaggctctt
     2341 gtggtagaaa cttttagctc aaacgtggta aaactatctc aacgtccttc cgtagacaac
     2401 tggtgagcag tgcttctgta acactgcaga tctccaatga taaatcgata ccgtactaac
     2461 tgcccgcact ataatgaact ctttgtatcc gtaactattt aaaatattca ttcatttcta
     2521 taagctatat gtttatattt agattagagg ggttaaaaga aagtttttct cgaagctt
//