From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Murine cytomegalovirus enhancer DNA sequence.
Date: 21 Dec 92 20:43:17 GMT
Distribution: bionet
Checksum: 46867 4

LOCUS       HS5ENHANC    1473 bp ds-DNA             VRL       20-NOV-1992
DEFINITION  Murine cytomegalovirus enhancer DNA sequence.
ACCESSION   L06570
KEYWORDS    enhancer; expression vector.
SOURCE      Murine cytomegalovirus DNA.
  ORGANISM  Murine cytomegalovirus
            Viridae; ds-DNA enveloped viruses; Herpesviridae;
            Betaherpesvirinae.
REFERENCE   1  (bases 1 to 1473)
  AUTHORS   Dorsch-Haesler,K., Keil,G.M., Weber,F., Jasin,M., Schaffner,W. and
            Koszinowski,U.H.
  TITLE     A long and complex enhancer activates transcription of the gene
            coding for the highly abundant immediate early mRNA in murine
            cytomegalovirus
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 82, 8325-8329 (1985)
  STANDARD  full automatic
REFERENCE   2  (bases 1 to 1473)
  AUTHORS   Messerle,M., Keil,G.M. and Koszinowski,U.H.
  TITLE     Structure and expression of murine cytomegalovirus immediate-early
            gene 2
  JOURNAL   J. Virol. 65, 1638-1643 (1991)
  STANDARD  full automatic
REFERENCE   3  (sites)
  AUTHORS   Messerle,M. and Koszinowski,U.H.
  TITLE     Highly efficient mammalian expression vectors based on the murine
            cytomegalovirus enhancer - an alternative to human cytomegalovirus
            enhancer expression vectors
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
COMMENT     [1] reports nucleotides 584-1473; [2] reports nucleotides 1-95;
            Messerle et al. (1992) reports features only.
FEATURES             Location/Qualifiers
     misc_signal     complement(49)
     TATA_signal     complement(73..77)
     repeat_region   300..1100
     TATA_signal     1396..1400
                     /note="putative"
     misc_signal     1424
BASE COUNT      395 a    329 c    372 g    377 t
ORIGIN      
        1 gttaacgtag gtagggtcgt atcgagccgc ggtccgtcct gctctgggct cgaatggcat
       61 gggggacagc aattatatgg ttaactccgc ccgttttatg actagaacca atagttttta
      121 atgccaaatg cactgaaatc ccctaatttg caaagccaaa cgccccctat gtgagtaata
      181 cggggacttt ttacccaatt tcccacgcgg aaagccccct aatacactca tatggcatat
      241 gaatcagcac ggtcatgcac tctaatggcg gcccataggg actttccaca tagggggcgt
      301 tcaccatttc ccagcatagg ggtggtgact caatggcctt tacccaagta cattgggtca
      361 atgggaggta agccaatggg tttttcccat tactggcaag cacactgagt caaatgggac
      421 tttccactgg gttttgccca agtacattgg gtcaatggga ggtgagccaa tgggaaaaac
      481 ccattgctgc caagtacact gactcaatag ggactttcca atgggttttt ccattgttgg
      541 caagcatata aggtcaatgt gggtgagtca atagggactt tccattgtat tctgcccagt
      601 acataaggtc aatagggggt gaatcaacag gaaagtccca ttggagccaa gtacactgcg
      661 tcaataggga ctttccattg ggttttgccc agtacataag gtcaataggg gatgagtcaa
      721 tgggaaaaac ccattggagc caagtacact gactcaatag ggactttcca ttgggttttg
      781 cccagtacat aaggtcaata gggggtgagt caacaggaaa gttccattgg agccaagtac
      841 attgagtcaa tagggacttt ccaatgggtt ttgcccagta cataaggtca atgggaggta
      901 agccaatggg tttttcccat tactggcacg tatactgagt cattagggac tttccaatgg
      961 gttttgccca gtacataagg tcaatagggg tgaatcaaca ggaaagtccc attggagcca
     1021 agtacactga gtcaataggg actttccatt gggttttgcc cagtacaaaa ggtcaatagg
     1081 gggtgagtca atgggttttt cccattattg gcacgtacat aaggtcaata ggggtgagtc
     1141 attgggtttt tccagccaat ttaattaaaa cgccatgtac tttcccacca ttgacgtcaa
     1201 tgggctattg aaactaatgc aacgtgacct ttaaacggta ctttcccata gctgattaat
     1261 gggaaagtac cgttctcgag ccaatacacg tcaatgggaa gtgaaagggc agccaaaacg
     1321 taacaccgcc ccggttttcc cctggaaatt ccatattggc acgcattcta ttggctgagc
     1381 tgcgttctac gtgggtataa gaggcgcgac cagcgtcggt accgtcgcag tcttcggtct
     1441 gaccaccgta gaacgcagag ctcctcgctg cag
//