From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Streptomyces halstedii hydroxylase (ORFC) gene, 5' end cds; ORFA,
Date: 6 Nov 92 18:35:59 GMT
Distribution: bionet
Checksum: 30967 12

LOCUS       STMHYDROXY   5224 bp ds-DNA             BCT       02-NOV-1992
DEFINITION  Streptomyces halstedii hydroxylase (ORFC) gene, 5' end cds; ORFA,
            complete cds; ORFB, complete cds; ketoacyl synthase (ORF1 and ORF2)
            genes, complete cds's; acyl carrier protein (ORF3) gene, complete
            cds; cyclase (ORF4) gene, 5' end cds.
ACCESSION   L05390
KEYWORDS    acyl carrier protein; cyclase; hydroxylase; ketoacyl synthase.
SOURCE      Streptomyces halstedii (library: NRRL 2381 in cosmid cos4) DNA.
  ORGANISM  Streptomyces halstedii
            Prokaryotae; Firmicutes; Streptomycetaceae.
REFERENCE   1  (bases 1 to 5224)
  AUTHORS   Blanco,G., Brian,P., Pereda,A., Mendez,C., Salas,J.A. and
            Chater,K.F.
  TITLE     Hybridization and DNA sequence analyses suggest an early
            evolutionary divergence of related biosynthetic gene sets for
            polyketide antibiotics and spore pigments in Streptomyces SPP.
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     CDS             complement(1..>226)
                     /function="hydroxylation"
                     /note="ORFC"
                     /note="putative"
                     /citation=[1]
                     /product="hydroxylase"
                     /codon_start=1
                     /translation="MNARADRAGDTVHRVPVLVVGGSLVGLSTSVFLGRLGVRHMLVE
                     RHAGTSVHPRGRGNNVRTMEVYRAAGVEQGI"
     CDS             452..1594
                     /note="ORFA"
                     /citation=[1]
                     /product="putative"
                     /codon_start=1
                     /translation="MTVSAAVSTVPDRVPLTVFDGSRVRVVLMLDIRDGTQAEVLDAY
                     ERMSDRVAAVPGHISDQLCQSLENPTQWLITSEWESAPEFLAWANSEEHLEMVRPLEP
                     YVRGTHSMRYSVLRETAEERAGAGAAARGALQPRPRIGDNVVRHAVTYTVKPDSVTEV
                     VKILSAYTSPEVRVDDTTRLVRTSLFLYGNRVVRAIEVRGDLQAALRHVARQPEVRAV
                     EEALTPHIEQDRDLTDPRSARLFFTRAALPAVHHVVSGRGTGGDTQRCALYYPAHPGA
                     GPALARLLARQGEATVGDPGSPVVACTVFHRDDLVVRLVDTAGAPERAPGAVLALHEP
                     DALAEAGRLLDAAALGADGPPDDRALPTFLAHARMRPLTDRQSPAS"
     CDS             1629..2045
                     /note="ORFB"
                     /citation=[1]
                     /product="putative"
                     /codon_start=1
                     /translation="MTEQQARIVAFDDVPPNRRRGGDVRALLTPTTAGATSGFMGVAV
                     VRPGERISEHYHPYSEEFVYVTAGAFEVDLDDVPHPLRTGQGLLIPKDVRHRFRNTGD
                     VEARLVFHLGPLAPRPDLGHVDTEETDETAPAGVVS"
     CDS             2042..3310
                     /function="condensation of acylCoA derivatives"
                     /standard_name="KS"
                     /note="ORF1"
                     /note="putative"
                     /citation=[1]
                     /product="ketoacyl synthase"
                     /codon_start=1
                     /translation="MSRRVVVTGIGVVAPGGIGAARFWDLLAGGRTATRRISLFDPAR
                     LRSQIAAECDFDPSAHGLDDETVRRCDRYVQFALVATAEAVRDAGLDTTREDPWRMGA
                     VLGTAVGGTTRLEHDYVLVSEGGSRWDVDHRRAEPHLHRAFAPSTLASTVAETFGAQG
                     PVQTVSTGCTSGLDAVGYAYHAIAEGRADVCLAGASDSPISPITMACFDAIKATSPSN
                     DDPEHASRPFDARRNGFVMGEGGAVLVLEELEHARARGADVYCELAGYATFGNAHHMT
                     GLTREGLEMARAIDTALDMARLDGTDIDYVNAHGSGTQQNDRHETAAVKRSLGEHAYR
                     TPMSSIKSMVGHSLGAIGSIEVVACVLALAHQVVPPTANYETPDPECDLDYVPREARE
                     RELRSVLSVGSGFGGFQSAVVLTGPERRLR"
     CDS             3307..4551
                     /function="condensation of acylCoA derivatives"
                     /standard_name="KS"
                     /note="ORF2"
                     /note="putative"
                     /citation=[1]
                     /product="ketoacyl synthase"
                     /codon_start=1
                     /translation="MSAPRRAVVTGLGVVAPHGIGAETFWKTAVDGTSSLARIDREGC
                     GHLPLKIAGQVPDFDPAALIEDTYLVQTDRFTHFAMAATQLALDDARLSRADIDSPYS
                     VGVVTAAGSGGGEFGQRELQKLWGQGSKYVGPYQSIAWFYAASTGQISIRGGFKGPCG
                     VVAADEAGGLDALAHAALAVRRGTATVVAGATEAPLAPYSMVCQLGYPELSRSADPGR
                     AYRPFTSAACGFVPAEGGAMFVLEEEGAARERGADARATVAGHAATFTGASRWEESRA
                     GLAHAIGTALARAGCRPQDVDVVFADALGVPEADRAEALALADALGPHARRVPVTAPK
                     AGIGRAFCAAAVLDVATALLAMEHELIPPTPHVLDVCHDLDLVVGRARPARPRTALVL
                     SRGLMGNNSALVLRRGAAPFPE"
     CDS             4617..4895
                     /standard_name="ACP"
                     /note="ORF3"
                     /note="putative"
                     /citation=[1]
                     /product="acyl carrier protein"
                     /codon_start=1
                     /translation="MTDMTERVGTQVTFEELSALMKRTAGVHVEPPDLRARAEEGFDG
                     FGLDSLGLLGIVAELEKKHGVGLPEQVERCKTPAEFLAQVNATLRTAV"
     CDS             4897..>5224
                     /note="ORF4"
                     /note="putative"
                     /citation=[1]
                     /product="cyclase"
                     /codon_start=1
                     /translation="MAGHTENEIVIAAPLDLVWDMTNDVENWPRLFSEYASAEILERE
                     GDRVRFRLTMHPDDEGRVWSWVSERVADRASLTVRAHRVETGPFQFMDIQWVYEQTPE
                     GVLMRWI"
BASE COUNT      710 a   1999 c   1848 g    667 t
ORIGIN      
        1 ggatcccctg ctcgacgccg gcggcccggt acacctccat cgtgcggacg ttgttcccgc
       61 gcccgcgagg gtggacggag gtgccggcgt gacgctccac cagcatgtgc cgcaccccga
      121 gccggcccag gaacacggac gtcgacaggc ccacgagcga tccgccgacg acgaggaccg
      181 gaaccctgtg gaccgtgtcc ccggcccgat cggctcttgc gttcatcttt ctcctccagc
      241 gcgtgatgtc cgcccactcg gccggtttcg gccggggtca tgcatgcccc gcgaggctgg
      301 agcgcggtgc gccggggacc acacttcacc cgcttaaccc gctgcgttcg cgcaggggca
      361 cggcacgccc gacgatcgtg ctcacgggcc gacgcaccgt catgtgacgc gtcggccgcc
      421 ttaccgttcc tccaggaaga ggtgcgcctc aatgacggtc tctgccgctg tgtccacggt
      481 cccggaccgt gtccccctca ccgtgttcga cggttcccgg gtgcgggtcg tgctgatgct
      541 ggacatccgc gacgggacgc aagcggaggt cctggacgcc tacgagcgga tgtccgaccg
      601 ggtcgccgcc gtgccggggc acatcagcga ccagctgtgc cagtcgctgg agaaccccac
      661 ccagtggctc atcaccagcg agtgggagag cgcaccggag ttcctcgcct gggccaacag
      721 cgaggaacac ctggagatgg tccgtcccct ggagccctac gtccgcggca cccactcgat
      781 gcgctactcg gtgctgcgcg agacggccga ggagcgggcc ggggcgggtg cggcggcccg
      841 gggcgcgctg cagccccggc cgcgcatcgg cgacaacgtg gtccggcacg ccgtcaccta
      901 caccgtcaag cccgacagcg tcaccgaggt cgtgaagatc ctctccgcct acacctcgcc
      961 cgaggtgcgc gtggacgaca ccacgcggct cgtgcgcacc tccctcttcc tgtacggcaa
     1021 ccgggtcgtc cgggcgatcg aggtgcgggg cgacctgcag gccgccctgc gccacgtggc
     1081 ccggcagccg gaggtgcgcg ccgtcgagga agccctcacc ccgcacatcg aacaggaccg
     1141 ggacctcacc gacccgcggt ccgcccggct gttcttcacc cgggccgcgc tgccggccgt
     1201 ccaccacgtg gtgtccgggc gcgggacggg cggcgacacg cagcggtgcg cgctgtacta
     1261 cccggcccac cccggcgccg gaccggcgct cgcccggctg ctggcgcggc agggcgaggc
     1321 caccgtgggc gacccgggca gtccggtcgt cgcctgcacc gtcttccacc gcgacgacct
     1381 cgtcgtacgg ctcgtcgaca cggcgggcgc accggagcgc gcgcccgggg ccgtcctggc
     1441 cctgcacgag ccggacgccc tcgccgaggc cgggcggctg ctggacgccg ccgcgctcgg
     1501 cgccgacggc cccccggacg accgggcgct gccgacgttc ctcgcgcacg cccggatgcg
     1561 gcctctgaca gaccgtcagt cgccggcctc ctgacccccc gctcgcccga cctcagggag
     1621 tgaccgacat gacagaacag caggcacgca tcgtcgcctt cgacgacgtc ccgcccaacc
     1681 ggcggcgcgg cggcgacgtc cgggccctgc tcacgcccac gaccgcgggg gcgaccagcg
     1741 gcttcatggg cgtggccgtc gtacggcccg gagaacgcat ctccgagcac taccacccgt
     1801 actccgagga gttcgtgtac gtcaccgccg gcgccttcga ggtggacctg gacgacgtgc
     1861 cgcatcccct gcgcaccggg cagggcctgc tcatccccaa ggacgtgcgc caccgcttcc
     1921 gcaacaccgg cgacgtcgag gcgcgcctcg tcttccacct gggtccgctg gccccccggc
     1981 cggacctcgg gcacgtcgac accgaggaga ccgacgagac cgcgccggcc ggggtggtgt
     2041 catgagccgc cgggtcgtcg tcaccggcat aggcgtcgtc gccccgggcg gcatcggcgc
     2101 ggcccggttc tgggacctgc tggccggcgg gcgtacggcg acgcgccgga tctccctgtt
     2161 cgacccggcg cgcctgcgct cgcagatcgc cgccgagtgc gacttcgacc cgtccgcgca
     2221 cggcctggac gacgagacgg tccggcggtg cgaccggtac gtgcagttcg cgctggtcgc
     2281 caccgccgag gcggtccgcg acgcgggcct ggacaccacg cgcgaggacc cctggcgcat
     2341 gggggccgtc ctcggcacgg cggtcggcgg caccacccgc ctggagcacg actacgtcct
     2401 ggtcagcgag ggcggctcgc gctgggacgt ggaccaccgg cgggccgagc cgcacctgca
     2461 ccgcgccttc gcccccagca cgctcgcctc caccgtcgcc gagaccttcg gcgcgcaggg
     2521 cccggtgcag accgtctcca ccggctgcac gtccgggctg gacgcggtgg ggtacgccta
     2581 ccacgccatc gccgagggcc gtgccgacgt gtgcctggcg ggcgcctcgg actcgccgat
     2641 atcgccgatc accatggcgt gcttcgacgc catcaaggcg acctcgccca gcaacgacga
     2701 cccggagcac gcctcccgcc ccttcgacgc ccgccgcaac gggttcgtga tgggcgaggg
     2761 cggcgcggtg ctcgtgctgg aggagctgga gcacgcccgg gcccgcggcg cggacgtcta
     2821 ctgcgagctc gccggctacg ccaccttcgg caacgcccac cacatgaccg ggctcacccg
     2881 ggagggcctg gagatggcgc gggccatcga caccgcgctg gacatggccc gcctggacgg
     2941 cacggacatc gactacgtca acgcgcacgg ctccggcacc cagcagaacg accggcacga
     3001 gaccgcggcg gtcaagcggt cgctgggcga gcacgcgtac cggaccccga tgagctcgat
     3061 caagtcgatg gtgggccact cgctcggcgc gatcggctcg atcgaggtcg tcgcctgcgt
     3121 cctcgccctg gcgcaccagg tggtgccgcc cacggccaac tacgagacac cggaccccga
     3181 gtgcgacctg gactacgtgc cgcgcgaggc acgcgagcgg gagctgcgca gcgtgctgtc
     3241 ggtgggcagc ggcttcggcg gcttccagtc cgcggtcgtg ctgaccggac cggagaggag
     3301 gctgagatga gcgcaccccg gcgagccgtc gtcaccggac tcggagtggt ggcaccccac
     3361 ggcatcggtg ccgagacgtt ctggaagacg gccgtggacg gcaccagcag cctggcccgg
     3421 atcgaccggg agggctgcgg ccacctgccc ctgaagatcg ccggccaggt ccccgacttc
     3481 gacccggccg ccctgatcga ggacacctac ctcgtccaga ccgaccgctt cacccacttc
     3541 gcgatggcgg ccacccagct cgccctcgac gacgcccggc tctcccgcgc cgacatcgac
     3601 tcgccgtact cggtgggcgt ggtgacggcc gcgggctccg gcggcggcga gttcggccag
     3661 cgcgagctgc agaaactgtg gggccagggc tcgaagtacg tcggccccta ccagtcgatc
     3721 gcctggttct acgcggcgag caccggccag atctccatcc gcggcggctt caagggcccc
     3781 tgcggcgtgg tggccgccga cgaggccggc ggcctggacg ccctcgcgca cgccgcgctg
     3841 gcggtacggc gcggcaccgc caccgtcgtc gccggcgcga ccgaggcccc gctggccccg
     3901 tactcgatgg tctgccagct gggttacccg gagctcagcc gcagcgccga cccgggccgg
     3961 gcctaccgtc ccttcacctc cgccgcctgc gggttcgtgc ccgccgaggg cggggcgatg
     4021 ttcgtcctgg aggaggaggg cgcggcacgc gagcgcggcg ccgacgcgcg ggcgacggtg
     4081 gccggccacg cggccacgtt caccggcgcc tcccgctggg aggagtccag ggccggcctg
     4141 gcgcacgcga tcggcacggc gctggcgcgg gccggctgcc gtccgcagga cgtggacgtc
     4201 gtgttcgccg acgccctcgg cgtgccggag gccgaccggg ccgaggccct ggccctggcc
     4261 gacgcgctcg gcccgcacgc gcggcgggtc cccgtcaccg ccccgaaggc gggcatcggc
     4321 cgggcgttct gcgcggccgc ggtgctcgac gtggcgaccg cgctgctcgc catggagcac
     4381 gagctgatcc cgcccacccc ccatgtgctc gacgtctgcc acgacctgga cctggtggtc
     4441 ggccgggcgc gtcccgcccg gccgcgcacc gcgctggtgc tcagccgcgg actcatgggc
     4501 aacaactcgg cgctcgtcct gcgcaggggc gccgcgccgt tccccgagta agtaccccga
     4561 acaggtgtct cacgtcccct tcgggcgcgg gcacccgagt caaggagctc aaccacatga
     4621 ccgacatgac cgaacgcgtg ggcacccagg tgaccttcga ggaactgtcc gccctgatga
     4681 agcgcaccgc gggcgtgcac gtggaaccgc ctgacctgcg ggcgcgggcc gaggagggct
     4741 tcgacggctt cggcctggac tccctgggcc tgctgggcat cgtggccgag ctggagaaga
     4801 agcacggcgt gggactgccg gagcaggtgg agcgctgcaa gacgcccgcg gagttcctcg
     4861 cgcaggtgaa cgccaccctc aggacggcgg tgtgacatgg ccgggcacac cgagaacgag
     4921 atcgtcatcg ccgcgccgct ggacctggtc tgggacatga ccaacgacgt cgagaactgg
     4981 ccgcggctgt tcagcgagta cgcctccgcc gagatcctgg agcgcgaggg cgaccgcgtc
     5041 cgcttccggc tcaccatgca cccggacgac gagggccggg tgtggagctg ggtctccgaa
     5101 cgcgtcgccg accgcgcctc cctgacggtc cgcgcccacc gcgtggagac cggccccttc
     5161 cagttcatgg acatccagtg ggtgtacgag cagacgcccg agggcgtgct gatgcgctgg
     5221 atcc
//