HDTenEightyP/NetNews huggingface dataset:
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Eimeria bovis developmental mRNA.
Date: 18 Aug 92 10:00:47 GMT
Distribution: bionet
Checksum: 21282     1

LOCUS       EIMDEVGENE    163 bp ss-mRNA            INV       18-AUG-1992
DEFINITION  Eimeria bovis developmental mRNA.
ACCESSION   M98840
KEYWORDS    .
SOURCE      Eimeria bovis (library: lambda gt11, ATCC CCL44) merozoite lamina
            propria cDNA to mRNA.
  ORGANISM  Eimeria bovis
            Eukaryota; Animalia; Protozoa; Apicomplexa; Perkinsea; Coccidia;
            Eucoccidiidae; Eimeriina; Eimeriidae.
REFERENCE   1  (bases 1 to 163)
  AUTHORS   Abrahamsen,M.S., Clark,T.G., Mascolo,P., Speer,C.A. and White,M.W.
  TITLE     Developmental Gene Expression in Eimeria bovis
  JOURNAL   Mol. Biochem. Parasitol. (1992) In press
  STANDARD  full staff_entry
BASE COUNT       47 a     31 c     38 g     47 t
ORIGIN
        1 aagggaagta aaggtgctca gggtcttacc gtcgggccat agggttcctc atattcaagg
       61 agttttctat tttaaaaagt cttacattag agacattagg aaagtcgtta caccattcat
      121 gcaggacgga atttacccga caaggaattt tgctaccttt ggc
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human immunodeficiency virus type 2 (73ENVD21) envelope fragment.
Date: 18 Aug 92 10:01:21 GMT
Distribution: bionet
Checksum: 23432     2

LOCUS       HIV7ENVD21    453 bp ss-RNA             VRL       18-AUG-1992
DEFINITION  Human immunodeficiency virus type 2 (73ENVD21) envelope fragment.
ACCESSION   M87143
KEYWORDS    envelope protein.
SOURCE      Human immunodeficiency virus type 2 short-term cultured blood
            mononuclear cells (9/90 sample) RNA.
  ORGANISM  Human immunodeficiency virus type 2
            Viridae; ss-RNA enveloped viruses; Positive strand RNA virus;
            Retroviridae; Lentivirinae.
REFERENCE   1  (bases 1 to 453)
  AUTHORS   Gao,F., Yue,L., White,A.T., Pappas,P.G., Barchue,J., Hanson,A.P.,
            Greene,B.M., Sharp,P.M., Shaw,G.M. and Hahn,B.H.
  TITLE     Human infection by genetically-diverse SIVsm-related HIV-2 in West
            Africa
  JOURNAL   Nature (1992) In press
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     misc_feature    1..453
                     /note="PCR derived HIV-2 envelope fragment"
                     /note="putative"
                     /citation=[1]
BASE COUNT      161 a     77 c    109 g    106 t
ORIGIN
        1 gacgtggtca agagacaaca agaaatgttg cgactgaccg tctggggaac aaaaaatctc
       61 cgggcaagag tcactgctat tgagaaatac ttaaaggacc aggcgcaact aaattcatgg
      121 ggatgcgcgt ttaggcaagt ctgccacact actgtaccat gggtaaatga cagcttgaca
      181 cctgattggg acaacatgac gtggcaacaa tgggaaaaac aaatccgctc cctggaggca
      241 aatatcagtg aaagtctaga acaggcacaa atccagcaag aaaagaacat gtatgaatta
      301 caaaaattaa atagctggga tgtttttggc aactggtttg atttagcctc ctgggtcaaa
      361 tatattcagt atggagttta tatagtagta ggaatagtag ctctcagaat aataatatat
      421 gtagtacaaa tgataggtag acttagaaga ggt
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Lentinula edodes ribosomal RNA (6LR0R) large subunit, partial.
Date: 19 Aug 92 10:01:23 GMT
Distribution: bionet
Checksum: 45553     2

LOCUS       LELRRLSB      236 bp ss-rRNA            RNA       19-AUG-1992
DEFINITION  Lentinula edodes ribosomal RNA (6LR0R) large subunit, partial.
ACCESSION   M98640
KEYWORDS    large subunit ribosomal RNA.
SOURCE      Lentinula edodes (library: Duke culture collection 607) cDNA to
            rRNA.
  ORGANISM  Lentinula edodes
            Eukaryota; Fungi; Amastigomycota; Basidiomycetes.
REFERENCE   1  (bases 1 to 236)
  AUTHORS   Hibbett,D.S. and Vilgalys,R.
  TITLE     Phylogenetic relationships of the basidiomycete genus Lentinus
            inferred from molecular and morphological characters
  JOURNAL   Unpublished (1992)
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     rRNA            <1..>236
                     /note="Approximately aligns to region between positions 70
                     to 306in Saccharomyces cerevisiae large subunit nuclear
                     rRNA."
                     /product="large subunit ribosomal RNA"
                     /citation=[1]
BASE COUNT       62 a     43 c     66 g     65 t
ORIGIN
        1 aaggattccc ctagtaactg cgagtgaagc gggaaaagct caaatttaaa atctggcagt
       61 ctctgattgt ccgagttgta atttagagaa gtgttacccg tgttggaccg tgtacaagtc
      121 tcctggaatg gagcgtcata gagggtgaga atcccgtctt tgatacggat cccaatacat
      181 tgtggtacac tctcgaagag tcgagttgtt tgggaatgca gctctaaatg ggtggt
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human chromosome 4 sequence-tagged site STS4-418
Date: 21 Aug 92 10:10:07 GMT
Distribution: bionet
Checksum: 41165     3

LOCUS       HUM4STS418    453 bp ds-DNA             PRI       21-AUG-1992
DEFINITION  Human chromosome 4 sequence-tagged site STS4-418
ACCESSION   L00848
KEYWORDS    STS; human chromosome 4; sequence tagged site.
SOURCE      Homo sapiens, clone p4-392 from a chromosome 4 plasmid library;
            vector pBluescript II KS + (Stratagene)
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 453)
  AUTHORS   Goold,R.D., diSibio,G., Dugaiczyk,A., Smith,K.A., Xu,H.,
            Magrane,G., Lang,D.B., Cox,D.R., Masters,S.B. and Myers,R.M.
  TITLE     Sequence-Tagged Sites for Human Chromosome 4
  JOURNAL   Unpublished (1992) see COMMENT for author address
  STANDARD  full automatic
COMMENT     PCR components: 25 ng of human genomic DNA, 10 pmol of each
            oligonucleotide, 200 micro-M dNTPs, 0.25 U Taq  polymerase (Cetus)
            in 10 micro-l of 50 mM KCl-20 mM  Tris-HCl, pH 8.3 (at room temp),
            2.5 mM MgCl-2.  Initial denaturation at 94degC for 1.5 min, then 30
            cycles of  94degC for 15 sec, 62degC for 23 sec, and 72degC for 30
            sec, followed by a final extension at 72degC for 3.5 min, using a
            Perkin-Elmer 9600 thermocycler. PCR-amplified product size 185 bp.
            Sequence submitted by:
            Human Genome Mapping Center
            Box 0925
            University of California San Francisco
            San Francisco, CA 94143-0925  USA
            Phone: (415) 502-1612   Fax: (415) 476-8391
            e-mail: hgmcprobes@cgl.ucsf.edu
FEATURES             Location/Qualifiers
     misc_feature    109..132
                     /label=Primer1
     misc_feature    complement(269..293)
                     /label=Primer2
BASE COUNT      155 a     87 c     62 g    148 t      1 others
ORIGIN
        1 gatctttgca cattaacaca tgagacgttg gtgtttctaa cagttacaga gaatgcaaag
       61 tcaccctgca aaaggattcc aaaatgtaca aaaattcaca ctttcaacag cacatttctc
      121 tctaacactc agatgactgc tcaaaaattt taacctctca caatctaaaa gatgaaagtg
      181 tatctcattg ctattttatt ttttccgtta tgagtatatt taggcatttt ttcacatgtc
      241 tactgaaaga aatggctaaa aaaagtttcc tttcctgtga actttctatt catgtcgtta
      301 ttattgagct atatttttct tatggattgg gtacgacctc tttttaacat taggaaaagt
      361 ggcacctaaa gaaaaacact taaacatcaa angttttctt aaaaaacaac acatgagggt
      421 ttggtttaca actttacaca cacacacaca caa
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Homo sapiens DNA-binding protein (ZNF) gene, partial cds.
Date: 21 Aug 92 10:11:59 GMT
Distribution: bionet
Checksum: 19646     2

LOCUS       HUMZNFP       291 bp ds-DNA             PRI       21-AUG-1992
DEFINITION  Homo sapiens DNA-binding protein (ZNF) gene, partial cds.
ACCESSION   M88372
KEYWORDS    DNA binding protein; zinc finger protein.
SOURCE      Homo sapiens Placenta DNA.
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 291)
  AUTHORS   Bray,P.L., Lichter,P., Thiesen,H.J., Ward,D.C. and Dawid,I.B.
  TITLE     Characterization and mapping of human genes encoding zinc finger
            proteins
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 88, 9563-9567 (1991)
  STANDARD  full automatic
REFERENCE   2  (bases 1 to 291)
  AUTHORS   Lichter,P., Bray,P., Ried,T., Dawid,I.B. and Ward,D.C.
  TITLE     Clustering of C2-H2 zinc finger motif sequences within telomeric
            and fragile site regions of human chromosomes
  JOURNAL   Genomics 13, 999-1007 (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     CDS             1..291
                     /standard_name="zinc finger motif"
                     /product="DNA binding protein"
                     /gene="ZNF"
                     /note="putative"
                     /codon_start=1
                     /translation="HQRTHTREKPYECNACGEAFIRSKSLARHQVLHTGKKPYKCNAC
                     GRAFCSNRNLIDHQRIHTWEKPYECSECGKAFSRSKCLIRHQSLHTGEKPYKC"
                     /partial
BASE COUNT       92 a     63 c     69 g     67 t
ORIGIN
        1 caccagagaa cccatactcg ggagaaacct tatgaatgca atgcgtgtgg agaggcattc
       61 attcgaagca aaagtcttgc tcgacatcag gtcctgcaca ctggtaagaa accttacaaa
      121 tgcaatgcgt gtgggagagc attctgttcc aatagaaatc tcattgacca tcagagaatc
      181 cacacttggg agaagcctta tgagtgtagt gaatgtggca aagccttcag tcggagtaaa
      241 tgtcttattc gacatcagag cctccatact ggggaaaagc catacaaatg t
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human chromosome 4 sequence-tagged site STS4-461
Date: 21 Aug 92 10:10:42 GMT
Distribution: bionet
Checksum: 45536     3

LOCUS       HUM4STS461    433 bp ds-DNA             PRI       21-AUG-1992
DEFINITION  Human chromosome 4 sequence-tagged site STS4-461
ACCESSION   L00877
KEYWORDS    STS; human chromosome 4; sequence tagged site.
SOURCE      Homo sapiens, clone p4-507 from a chromosome 4 plasmid library;
            vector pBluescript II KS + (Stratagene)
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 433)
  AUTHORS   Goold,R.D., diSibio,G., Dugaiczyk,A., Smith,K.A., Xu,H.,
            Magrane,G., Lang,D.B., Cox,D.R., Masters,S.B. and Myers,R.M.
  TITLE     Sequence-Tagged Sites for Human Chromosome 4
  JOURNAL   Unpublished (1992) see COMMENT for author address
  STANDARD  full automatic
COMMENT     PCR components: 25 ng of human genomic DNA, 10 pmol of each
            oligonucleotide, 200 micro-M dNTPs, 0.25 U Taq  polymerase (Cetus)
            in 10 micro-l of 50 mM KCl-20 mM  Tris-HCl, pH 8.3 (at room temp),
            2.5 mM MgCl-2.  Initial denaturation at 94degC for 1.5 min, then 30
            cycles of  94degC for 15 sec, 62degC for 23 sec, and 72degC for 30
            sec, followed by a final extension at 72degC for 3.5 min, using a
            Perkin-Elmer 9600 thermocycler. PCR-amplified product size 172 bp.
            Sequence submitted by:
            Human Genome Mapping Center
            Box 0925
            University of California San Francisco
            San Francisco, CA 94143-0925  USA
            Phone: (415) 502-1612   Fax: (415) 476-8391
            e-mail: hgmcprobes@cgl.ucsf.edu
FEATURES             Location/Qualifiers
     misc_feature    41..63
                     /label=Primer1
     misc_feature    complement(190..212)
                     /label=Primer2
BASE COUNT       94 a    124 c    122 g     87 t      6 others
ORIGIN
        1 gatccccagc tgggtgggga catctccacc cctcctggag gacctctctt acggagttca
       61 gtatttcctg tgctggcaca gagaaagtgc tgggcccaca aagcccctga actgcaggca
      121 ctggactcgg cctgaggggc tgactcggtc aggaggtcag gccactaaag cttccacagt
      181 gtgaccctga ctttcccgta acgattccac agaaaacccc gcacactggt caacctgctc
      241 acaagtgaca ggggnatggg ttctgcccac agtggattcc ggccgggggg tgctccgtta
      301 gatgcggnct gtcctccagg tcccgaggac atgcagttat ggacgtgcat tgnacagagn
      361 ccacagntcc tagatgagag caggaatgtt ccaggntgtc cctcatcaga accttcttac
      421 agtaaaagtc ggg
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human chromosome 4 sequence-tagged site STS4-100
Date: 21 Aug 92 10:06:28 GMT
Distribution: bionet
Checksum: 04902     3

LOCUS       HUM4STS100    626 bp ds-DNA             PRI       21-AUG-1992
DEFINITION  Human chromosome 4 sequence-tagged site STS4-100
ACCESSION   M95233
KEYWORDS    STS; human chromosome 4; sequence tagged site.
SOURCE      Homo sapiens, clone C4-67 from Los Alamos National Laboratory
            chromosome 4 cosmid library, plate 4-1-1R, pos 1B; vector SuperCos
            1 (Stratagene)
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 626)
  AUTHORS   Goold,R.D., diSibio,G., Dugaiczyk,A., Smith,K.A., Xu,H.,
            Magrane,G., Lang,D.B., Cox,D.R., Masters,S.B. and Myers,R.M.
  TITLE     Sequence-Tagged Sites for Human Chromosome 4
  JOURNAL   Unpublished (1992) see COMMENT for author address
  STANDARD  full automatic
COMMENT     PCR components: 25 ng of human genomic DNA, 10 pmol of each
            oligonucleotide, 200 micro-M dNTPs, 0.25 U Taq  polymerase (Cetus)
            in 10 micro-l of 50 mM KCl-20 mM  Tris-HCl, pH 8.3 (at room temp),
            2.5 mM MgCl-2.  Initial denaturation at 94degC for 1.5 min, then 30
            cycles of  94degC for 15 sec, 62degC for 23 sec, and 72degC for 30
            sec, followed by a final extension at 72degC for 3.5 min, using a
            Perkin-Elmer 9600 thermocycler. PCR-amplified product size 202 bp.
            Sequence contains short tandem repeat.
            Sequence submitted by:
            Human Genome Mapping Center
            Box 0925
            University of California San Francisco
            San Francisco, CA 94143-0925  USA
            Phone: (415) 502-1612   Fax: (415) 476-8391
            e-mail: hgmcprobes@cgl.ucsf.edu
FEATURES             Location/Qualifiers
     misc_feature    13..34
                     /label=Primer1
     misc_feature    complement(190..214)
                     /label=Primer2
BASE COUNT      160 a    136 c    118 g    190 t     22 others
ORIGIN
        1 ttatctcctg cttcacctga gtcgaaccca cttgcgcttc tctggaagtg ccagactgtt
       61 cntgtccccc ctctgcctgg gcttgctttt ctgccttgat gtcctggnaa gccctactcc
      121 cttcaaaacc cagcacaaac attttctctg tgaattattc tgttattatc acaggggatc
      181 ctcctttatg ccacatacta taatactttg agtcaatcaa tttnnatata tgtgttatat
      241 atgtgtacac acacacacac acacacacat atatatatat gtatctgtta gaaactttat
      301 attctgtatt acagatttta aaaaatgttt ttcttcctgt ctagacacta agtcccttga
      361 gggcagggaa tgggaaaagg aatgaatgtt gattacttac tctgtgtcag gtcctgaaca
      421 aggacttttg nnaatattat ttcatttaga gtctgtatct tcttccatat ctgggaggaa
      481 ggcagngaag aaagaaagga acaaaaggcc ngagaggaag gnngangtct tattccccat
      541 gtgttaacgc ttaggttccc cagttaacct gtgnaancca agcntacgtt tcgggggagg
      601 ngtnaggnan gccctntctc cnnccn
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Lentinus ponderosus ribosomal RNA (16LR3) large subunit, partial.
Date: 19 Aug 92 10:02:03 GMT
Distribution: bionet
Checksum: 17462     2

LOCUS       LENRRLSE      103 bp ss-rRNA            RNA       19-AUG-1992
DEFINITION  Lentinus ponderosus ribosomal RNA (16LR3) large subunit, partial.
ACCESSION   M98586
KEYWORDS    large subunit ribosomal RNA.
SOURCE      Lentinus ponderosus (library: Duke culture collection 592) cDNA to
            rRNA.
  ORGANISM  Lentinus ponderosus
            Eukaryota; Fungi; Eumycota; Basidiomycotina; Hymenomycetes;
            Agaricales; Tricholomataceae.
REFERENCE   1  (bases 1 to 103)
  AUTHORS   Hibbett,D.S. and Vilgalys,R.
  TITLE     Phylogenetic relationships of the basidiomycete genus Lentinus
            inferred from molecular and morphological characters
  JOURNAL   Unpublished (1992)
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     rRNA            <1..>103
                     /note="Aligns to region between positions 371 to 597
                     inSaccharomyces cerevisiae large subunit nuclear rRNA."
                     /product="large subunit ribosomal RNA"
                     /citation=[1]
BASE COUNT       17 a     21 c     35 g     30 t
ORIGIN
        1 attttccggt tgacgggcct gcatcgattt gaccgctgga taagggctag gggaatgtgg
       61 caccttcggg tgtgttatag cctctggtcg tatacagtgg tcg
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human chromosome 4 sequence-tagged site STS4-328
Date: 21 Aug 92 10:08:59 GMT
Distribution: bionet
Checksum: 39230     3

LOCUS       HUM4STS328    482 bp ds-DNA             PRI       21-AUG-1992
DEFINITION  Human chromosome 4 sequence-tagged site STS4-328
ACCESSION   L00798
KEYWORDS    STS; human chromosome 4; sequence tagged site.
SOURCE      Homo sapiens, clone p4-268 from a chromosome 4 plasmid library;
            vector pBluescript II KS + (Stratagene)
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 482)
  AUTHORS   Goold,R.D., diSibio,G., Dugaiczyk,A., Smith,K.A., Xu,H.,
            Magrane,G., Lang,D.B., Cox,D.R., Masters,S.B. and Myers,R.M.
  TITLE     Sequence-Tagged Sites for Human Chromosome 4
  JOURNAL   Unpublished (1992) see COMMENT for author address
  STANDARD  full automatic
COMMENT     PCR components: 25 ng of human genomic DNA, 10 pmol of each
            oligonucleotide, 200 micro-M dNTPs, 0.25 U Taq  polymerase (Cetus)
            in 10 micro-l of 50 mM KCl-20 mM  Tris-HCl, pH 8.3 (at room temp),
            2.5 mM MgCl-2.  Initial denaturation at 94degC for 1.5 min, then 30
            cycles of  94degC for 15 sec, 62degC for 23 sec, and 72degC for 30
            sec, followed by a final extension at 72degC for 3.5 min, using a
            Perkin-Elmer 9600 thermocycler. PCR-amplified product size 272 bp.
            STS sequence contains a simple sequence tandem repeat (CA).
            Sequence submitted by:
            Human Genome Mapping Center
            Box 0925
            University of California San Francisco
            San Francisco, CA 94143-0925  USA
            Phone: (415) 502-1612   Fax: (415) 476-8391
            e-mail: hgmcprobes@cgl.ucsf.edu
FEATURES             Location/Qualifiers
     misc_feature    9..35
                     /label=Primer1
     misc_feature    complement(257..280)
                     /label=Primer2
BASE COUNT      160 a     88 c     79 g    149 t      6 others
ORIGIN
        1 gatcaccaag gtatcttttt agtatcataa taagcatata aatttgnaaa aaatacacac
       61 acacacacac acacacacac acccctaaaa tgtggtttaa tcaatcgcag tttgctcaaa
      121 atgtcccatg tttagccaat ggaaacctct tcaggttggt tcccacatcc ttnngacagg
      181 acccaataat aattgataga ttcctggctt tctgtatgac aaaatgttcc aggtgcactt
      241 cccagactaa aaattagaca cttctcccaa gacctagttt cttttagtga gagaccacaa
      301 tctatattta gggtattatc ctttaatttc tatttngggg ggggttatgt tttataatgc
      361 acgtaatata ttaataatat gggtaaatac atattaagtt agaaatggca aacaaagaca
      421 tnttttttct tgaaagggat gnccgataca gaaagtttag aggttactta tttagggcat
      481 ag
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human chromosome 4 sequence-tagged site STS4-52
Date: 21 Aug 92 10:10:57 GMT
Distribution: bionet
Checksum: 19007     3

LOCUS       HUM4STS52     389 bp ds-DNA             PRI       21-AUG-1992
DEFINITION  Human chromosome 4 sequence-tagged site STS4-52
ACCESSION   M95310
KEYWORDS    STS; human chromosome 4; sequence tagged site.
SOURCE      Homo sapiens, clone L4-34 from the C25 lambda library (Pritchard,
            Genomics 4, 408, 1989); vector - Lambda Dash II (Stratagene)
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 389)
  AUTHORS   Goold,R.D., diSibio,G., Dugaiczyk,A., Smith,K.A., Xu,H.,
            Magrane,G., Lang,D.B., Cox,D.R., Masters,S.B. and Myers,R.M.
  TITLE     Sequence-Tagged Sites for Human Chromosome 4
  JOURNAL   Unpublished (1992) see COMMENT for author address
  STANDARD  full automatic
COMMENT     PCR components: 25 ng of human genomic DNA, 40 pmol of each
            oligonucleotide, 200 micro-M dNTPs, 0.25 U Taq  polymerase (Cetus)
            in 10 micro-l of 50 mM KCl-20 mM  Tris-HCl, pH 8.3 (at room temp),
            2.5 mM MgCl-2.  Initial denaturation at 94degC for 1.5 min, then 30
            cycles of  94degC for 15 sec, 64degC for 23 sec, and 72degC for 30
            sec, followed by a final extension at 72degC for 3.5 min, using a
            Perkin-Elmer 9600 thermocycler. PCR-amplified product size 213 bp.
            Sequence submitted by:
            Human Genome Mapping Center
            Box 0925
            University of California San Francisco
            San Francisco, CA 94143-0925  USA
            Phone: (415) 502-1612   Fax: (415) 476-8391
            e-mail: hgmcprobes@cgl.ucsf.edu
FEATURES             Location/Qualifiers
     misc_feature    28..52
                     /label=Primer1
     misc_feature    complement(217..240)
                     /label=Primer2
BASE COUNT      116 a     78 c     84 g     83 t     28 others
ORIGIN
        1 tcaacggatc cntcngacaa gaaacaaaca aggtaagctt tatagtcact ccttctgcct
       61 acaggtactn tctagaccta ctaaagagga gaaaacaagg agagtatgta tgagcttgca
      121 antagaggaa gccaaggtaa caattanaaa ttagtggaaa cacatgggat tgngcagcgc
      181 nntttttnca tttttgagag atctacagag ataaaaggtc cagaaatctg tntgggtttc
      241 cctcannatn ttctntncta ctaagctgca cgtgaatatg gcaganctcc atgagattgn
      301 gcaaatanga accaggacgc tgcantcaga aannttccca gaatgcannc ggccccggga
      361 gagnggntga cnctcactgc cncctcngg
//