From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Bacillus caldotenax pol gene for DNA polymerase.
Date: 9 Jan 93 05:45:42 GMT
Distribution: bionet
Checksum: 21519 7

LOCUS       BACPOLYTG    3329 bp ds-DNA             BCT       09-JAN-1993
DEFINITION  Bacillus caldotenax pol gene for DNA polymerase.
ACCESSION   D12982
KEYWORDS    DNA polymerase.
SOURCE      Bacillus caldotenax (strain YT-G) DNA.
  ORGANISM  Bacillus caldotenax
            Prokaryotae; Bacteria; Firmicutes; Endospore-forming rods and
            cocci; Bacillaceae.
REFERENCE   1  (bases 1 to 3329)
  AUTHORS   Uemori,T., Ishino,Y., Fujita,K., Asada,K. and Kato,I.
  TITLE     Cloning of the DNA polymerase gene of Bacillus caldotenax and
            characterization of the gene product
  JOURNAL   J. Biochem. (1993) In press
  STANDARD  full automatic
COMMENT     Submitted (24-AUG-1992) to DDBJ by: Yoshizumi Ishino Biotechnology
            Research Lab. Takara Shuzo Co., Ltd. 3-4-1 Seta, Otsu Shiga 520-21
            Japan Phone:  0775-43-7215 Fax:    0775-43-2494.
FEATURES             Location/Qualifiers
     CDS             315..2948
                     /gene="pol"
                     /note="ttg start codon"
                     /product="DNA polymerase"
                     /codon_start=1
                     /translation="MKKKLVLIDGSSVAYRAFFALPLLHNDKGIHTNAVYGFTMMLNK
                     ILAEEEPTHMLVAFDAGKTTFRHEAFQEYKGGRQQTPPELSEQFPLLRELLRAYRIPA
                     YELENYEADDIIGTLAARAEQEGFEVKVISGDRDLTQLASPHVTVDITKKGITDIEPY
                     TPEAVREKYGLTPEQIVDLKGLMGDKSDNIPGVPGIGEKTAVKLLRQFGTVENVLASI
                     DEIKGEKLKETLRQHREMALLSKKLAAIRRDAPVELSLDDIAYQGEDREKVVALFKEL
                     GFQSFLEKMESPSSEEEKPLAKMAFTLADRVTEEMLADKAALVVEVVEENYHDAPIVG
                     IAVVNEHGRFFLRPETALADPQFVAWLGDETKKKSMFDSKRAAVALKWKGIELCGVSF
                     DLLLAAYLLDPAQGVDDVAAAAKMKQYEAVRPDEAVYGKGAKRAVPDEPVLAEHLVRK
                     AAAIWALERPFLDELRRNEQDRLLVELEQPLSSILAEMEFAGVKVDTKRLEQMGEELA
                     EQLRTVEQRIYELAGQEFNINSPKQLGVILFEKLQLPVLKKSKTGYSTSADVLEKLAP
                     YHEIVENILQHYRQLGKLQSTYIEGLLKVVRPDTKKVHTIFNQALTQTGRLSSTEPNL
                     QNIPIRLEEGRKIRQAFVPSESDWLIFAADYSQIELRVLAHIAEDDNLMEAFRRDLDI
                     HTKTAMDIFQVSEDEVTPNMRRQAKAVNFGIVYGISDYGLAQNLNISRKEAAEFIERY
                     FESFPGVKRYMENIVQEAKQKGYVTTLLHRRRYLPDITSRNFNVRSFAERMAMNTPIQ
                     GSAADIIKKAMIDLNARLKEERLQARLLLQVHDELILEAPKEEMERLCRLVPEVMEQA
                     VTLRVPLKVDYHYGSTWYDAK"
BASE COUNT      841 a    811 c    959 g    718 t
ORIGIN      
        1 gtcgacaagg cgcgcacgcg cgattccggc ggaacggggt tgggcctggc gattgtgaaa
       61 catttggttg aggctcacca tggatatatt accgtagcga gcaaagtggg gcgcggcacc
      121 gtgttcacga tccattttcc aaagccgggg cggtagccgg cttcttttta tcatctccaa
      181 ctgagaagcc tgccattttt cagcgtgacg tgagcacggg atgaatccgc gcctcccatc
      241 atgttgggag agcgttcaag gcaagccgca ggcatggtac aataggacaa ggaagcatcc
      301 gaggagggat gagattgaaa aaaaagcttg ttttaatcga cggcagcagc gtggcgtacc
      361 gcgccttttt cgccttgccg cttttgcata acgacaaagg catccatacg aacgccgtct
      421 acgggtttac gatgatgttg aataaaattt tggcggaaga agagccaact catatgcttg
      481 tcgcgtttga cgccgggaaa acgacgttcc ggcatgaagc gtttcaagag tataaaggtg
      541 ggcgccagca gacgccaccg gagctgtcgg agcagtttcc gctgttgcgc gagctgctga
      601 gggcgtatcg catccccgcc tatgaactcg agaactacga agcggacgat attatcggaa
      661 cgcttgccgc ccgcgctgag caggaagggt ttgaggtgaa agtcatttcc ggcgaccgcg
      721 atctgaccca gctcgcctcc ccccatgtga cggtggacat tacgaaaaaa gggattaccg
      781 atatcgaacc gtacacgccg gaggcggtcc gcgaaaaata cggcttaact ccggaacaaa
      841 tcgttgattt gaaaggattg atgggcgaca aatcggacaa cattcccgga gtgccgggca
      901 tcggggaaaa gacggcggtc aagctgctca ggcaattcgg cacggtcgaa aacgtgcttg
      961 cctccattga cgagatcaaa ggcgaaaagt tgaaagaaac gctgcgccaa caccgggaga
     1021 tggcgctgtt aagcaaaaag ctcgccgcca ttcgccgcga cgccccggtc gagctctcgc
     1081 ttgatgacat cgcctatcaa ggggaagacc gggagaaagt ggtcgcttta tttaaagagc
     1141 ttgggtttca atcgttttta gagaaaatgg aatcgccgtc atcagaagag gaaaaaccgc
     1201 ttgccaagat ggcatttacg cttgctgacc gcgtgacgga ggagatgctt gccgacaagg
     1261 cggcgcttgt cgttgaagtg gtcgaggaaa attatcatga tgcgccgatc gtcggcatcg
     1321 ctgtggtcaa cgaacatgga cggtttttcc tgcgcccgga gacggcgctt gccgatccgc
     1381 agtttgtcgc ctggcttggt gatgaaacga agaaaaaaag catgtttgac tcaaagcgcg
     1441 cggcagtcgc cttgaaatgg aaaggaattg agctatgcgg cgtttccttt gatttattgc
     1501 tggccgccta tttgcttgat ccggcgcaag gtgttgatga tgtggctgcc gcagcaaaaa
     1561 tgaagcaata cgaagcggtg cgcccggatg aagcggtgta tggcaaaggg gcgaagcggg
     1621 ccgtgccgga tgagccagtg ctcgccgagc atttggtccg caaggcggcg gcgatttggg
     1681 cgctcgaacg gccgtttttg gatgagctgc gccgcaacga acaagatcgg ttgctcgtcg
     1741 agctcgagca gccgttgtct tcgattttgg cggaaatgga atttgccgga gtgaaagtgg
     1801 atacgaagcg gctcgaacag atgggcgaag agctcgccga gcagctgcgc acggtcgagc
     1861 agcgcattta tgagctcgcc ggccaagaat tcaacatcaa ttcaccgaaa cagctcggcg
     1921 tcattttatt tgaaaaactg cagctgcccg tcttgaaaaa aagcaaaacc ggctactcca
     1981 cttcggcgga tgtgcttgaa aaacttgcgc cttatcacga gatcgtggaa aacattttgc
     2041 aacattaccg ccagcttggc aagttgcagt cgacgtatat tgaaggattg ctgaaagtcg
     2101 tgcgacccga tacaaagaag gtgcatacga ttttcaatca ggcgttgacg caaaccggac
     2161 ggctcagctc gacggagccg aacttgcaaa acattccgat tcggcttgag gaaggacgga
     2221 aaatccgcca agcgttcgtg ccgtcggagt ctgattggct cattttcgct gccgactact
     2281 cgcaaattga gttgcgcgtc ctcgcccata ttgcggaaga tgacaattta atggaagcgt
     2341 tccgccgcga tttggatatc catacgaaaa cagcgatgga cattttccaa gtgagcgagg
     2401 acgaagtgac gcccaacatg cgccgtcagg cgaaggcggt caactttggg atcgtttacg
     2461 ggatcagtga ttacggcttg gcgcaaaact taaatatttc acgcaaagag gccgctgaat
     2521 tcatcgagcg ctacttcgaa agcttccctg gcgtgaagcg gtatatggaa aacattgtgc
     2581 aagaagcaaa acagaaaggg tatgtgacga cgctgctgca tcggcgccgc tatttgccgg
     2641 atattacgag ccgcaacttc aacgtccgca gctttgctga acggatggcg atgaacacgc
     2701 cgattcaagg gagcgccgct gacattatta aaaaggcgat gatcgatctg aacgccagac
     2761 tgaaggaaga gcggctgcaa gcgcgccttt tgctgcaggt gcatgacgag ctcattttgg
     2821 aggcgccgaa agaagagatg gagcggctgt gccggctcgt tccggaagtg atggagcaag
     2881 cggtcacact tcgcgtgccg ctcaaagtcg attaccatta cggctcgaca tggtatgacg
     2941 cgaaataaaa aggagtcttg gtgtgtggat cgccggcacc cctaaaaggc cggtgattta
     3001 aggggaaata ctgctctcca acagtgtttc tcaaattgaa aaaccttgca acaccatcac
     3061 ttcattcctt gtgatttctc ataaatcaag cgaatccatt gtttttcatc agccttctaa
     3121 gaaggcctgt gatggaatga aaaagcagtt tcacaacgac tcttctccag ttgagaagcc
     3181 ttgggacatc gagtcgtcct tctcaaccaa catgaccgat tttgcgaaaa tcagcgtttc
     3241 tcaccggcct tctaggcaga atctttcggt gcgacgattc tcggctgcaa ctcggatgaa
     3301 ttggagcgaa atcagctgcc gccccatgg
//