From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Influenza A virus genomic RNA for NS1 and NS2 protein, complete
Date: 21 Jul 92 10:04:30 GMT
Distribution: bionet
Checksum: 56057     4

LOCUS       FLANS         890 bp ss-RNA             VRL       21-JUL-1992
DEFINITION  Influenza A virus genomic RNA for NS1 and NS2 protein, complete
            cds.
ACCESSION   D10571 D90526
KEYWORDS    NS1 protein; NS2 protein; nonstructural protein.
SOURCE      Influenza virus type A (strain A/Aichi/2/68(Ao)) RNA.
  ORGANISM  Influenza virus type A
            Viridae; ss-RNA enveloped viruses; Negative strand RNA viruses;
            Orthomyxoviridae; Influenzavirus; Influenza A viruses.
REFERENCE   1  (sites)
  AUTHORS   Odagiri,T. and Tobita,K.
  TITLE     Mutation in NS2, a nonstructural protein of influenza A virus,
            exragenically causes aberrant replication and expression of the PA
            gene and leads to generation of degective interfering particles
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 87, 5988-5992 (1990)
  STANDARD  full staff_review
REFERENCE   2  (bases 1 to 890)
  AUTHORS   Odagiri,T., Tobita,K. and Ohta,S.
  TITLE     An amino acid substitution in the NS2 protein of influenza A virus
            suppresses complementary RNA synthesis of the PA poly-merase gene
            and leads to production of defective interfering particles.
  JOURNAL   Unpublished (1992)
  STANDARD  simple staff_entry
COMMENT     Data Kindly submitted in computer readable form by:
            Takato Odagiri
            Department of Virology
            Jichi Medical School
            Mianmi-kawach, Kawachi-gun
            Tochigi-ken 329-04
            Japan
            Phone:  0285-44-2111 x3153
            Fax:    0285-44-1827
            [1]  sites.
FEATURES             Location/Qualifiers
     CDS             join(27..56,529..864)
                     /product="NS2 protein"
                     /codon_start=1
                     /translation="MDSNTVSSFQDILLRMSKMQLGSSSEDLNGMITQFESLKLYRDS
                     LGEAVMRMGDLHSLQNRNGKWREQLGQKFEEIRWLIEEVRHRLKTTENSFEQITFMQA
                     LQLLFEVEQEIRTFSFQLI"
                     /partial
     exon            27..56
                     /number=1
     CDS             27..740
                     /product="NS1 protein"
                     /codon_start=1
                     /translation="MDSNTVSSFQVDCFLWHVRKQVVDQELGDAPFLDRLRRDQKSLR
                     GRGSTLGLNIEAATRVGKQIVERILKEESDEALKMTMASAPASRYLTDMTIEELSRDW
                     FMLMPKQKVEGPLCIRIDQAIMDKNVMLKANFSVIFDRLETLILLRAFTEEGAIVGEI
                     SPLPSLPGHTIEDVKNAIGVLIGGLEWNDNTVRVSKTLQRFAWGSSNENGRPPLTPKQ
                     KRKMARTVRSKVRRDKMAD"
     intron          57..528
     exon            529..864
                     /number=2
     variation       698
                     /type="A/Aichi/2/68"
                     /note="replace(698, g)"
BASE COUNT      290 a    175 c    213 g    212 t
ORIGIN      segment 8.
        1 agcaaaagca gggtgacaaa gacataatgg attctaacac tgtgtcaagt tttcaggtag
       61 attgcttcct ttggcatgtc cgaaaacaag ttgtagacca agaactaggt gatgccccat
      121 tccttgatcg gcttcgccga gatcagaagt ccctaagggg aagaggcagc actctcggtc
      181 taaacatcga agcagccacc cgtgttggaa agcagatagt agagaggatt ctgaaggaag
      241 aatccgatga ggcacttaaa atgaccatgg cctccgcacc tgcttcgcga tacctaactg
      301 acatgactat tgaggaattg tcaagggact ggttcatgct aatgcccaag cagaaagtgg
      361 aaggacctct ttgcatcaga atagaccagg caatcatgga taagaacgtc atgttgaaag
      421 cgaatttcag tgtgattttt gaccggctag agaccctaat attactaagg gctttcaccg
      481 aagagggagc aattgttggc gaaatctcac cattgccttc tcttccagga catactattg
      541 aggatgtcaa aaatgcaatt ggggtcctca tcggaggact tgaatggaat gataacacag
      601 ttcgagtctc taaaactcta cagagattcg cttggggaag cagtaatgag aatgggagac
      661 ctccactcac tccaaaacag aaacggaaaa tggcgagaac agttaggtca aaagttcgaa
      721 gagataagat ggctgattga agaagtgaga cacagattga agacaacaga gaatagtttt
      781 gagcaaataa catttatgca agccttacag ctactatttg aagtggaaca ggagataaga
      841 actttctcgt ttcagcttat ttaatgataa aaaacaccct tgtttctact
//