From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Mouse protein kinase inhibitor (testicular isoform) mRNA, complete
Date: 29 Dec 92 19:58:52 GMT
Distribution: bionet
Checksum: 29403 4

LOCUS       MUSPROKIN    1465 bp ss-mRNA            ROD       10-DEC-1992
DEFINITION  Mouse protein kinase inhibitor (testicular isoform) mRNA, complete
            cds.
ACCESSION   L02241
KEYWORDS    cAMP; cAMP dependent; protein kinase; protein kinase inhibitor;
            testicular protein.
SOURCE      Mus musculus (strain BALB/c, sub_species domesticus) (library:
            Uni-ZAP XR) neonatal brain cDNA to mRNA.
  ORGANISM  Mus musculus
            Eukaryota; Animalia; Chorqata; Vertebrata; Mammalia; Theria;
            Eutheria; Rodentia; Myomorpha; Muridae; Murinae.
REFERENCE   1  (bases 1 to 1465)
  AUTHORS   Scarpetta,M.A. and Uhler,M.D.
  TITLE     Evidence for two additional isoforms of the endogenous protein
            kinase inhibitor of cAMP-dependent protein kinase in mouse
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     5'UTR           1..464
     misc_difference replace(392..499,"ta")
                     /note="105 base pair deletion in clone PKI1.1"
     3'UTR           744..1465
     repeat_region   852..905
                     /rpt_type=direct
                     /rpt_type=tandem
                     /standard_name="dinucleotide GT repeat"
     misc_difference replace(1362,"t")
                     /note="found in clone PKI7.1"
     polyA_signal    1444..1449
     polyA_site      1465
     CDS             465..743
                     /evidence="EXPERIMENTAL"
                     /note="largest open reading frame reported; actaul start
                     codon unknown; testicular isoform"
                     /product="protein kinase inhibitor"
                     /codon_start=1
                     /translation="MGGGTSPEAQQDSVMRTDSSEMTDVESVITSFASSARAGRRNAL
                     PDIQSSLATSGSSDLPLKLEALAVKEDAKTKNEEKDQGQPKTPLNEGK"
BASE COUNT      413 a    315 c    355 g    382 t
ORIGIN      
        1 ggcacgagct taactgggca ggtcctgtgg agcctagctc agctcgcggg ggcgggaccg
       61 agcggctccc tccgccccac acattgctgc caccagctca gcccagccgc atccagagca
      121 gaggcgctgc aagctccagc cgtcggcagc tccgtgccgc agagaggccg gcagcaggcg
      181 ccagccgtga gggacctggc ccggcgccgc ccgcatctct tgcgcgcacg tggggtcatg
      241 atatactgga aaccacacaa gattagtctg tggaggcaga aaccataaag cccctgaaac
      301 caggttcgga gaggaacgat aaaggaaaaa ctccctttcg gcttcatcct tcacctgtct
      361 tagaaacaac agcacatttt aaccacagat ttcacctggg ccactggcac catggataag
      421 cacgctgatg cccctggggt caaaagaaaa caaaggagac cagcatggga ggcggcacga
      481 gcccagaagc ccagcaagat tctgtgatga ggacagattc atcagagatg actgatgtgg
      541 aatctgtgat caccagcttt gcgtcctcag caagggcagg ccgccgcaat gccttacccg
      601 acatccagag ttcactggct acaagtggat cctctgatct tccactgaaa ctggaagcgt
      661 tggccgtgaa ggaagatgca aaaacgaaga atgaagagaa agaccaaggc caaccaaaaa
      721 cacccctaaa tgaaggaaaa tgaaggttca gaattcatca agggtgttga ttttatgctc
      781 attgaaagac atagcagcta tgtattcctg gggtgtttga tctggtagta atcatggtaa
      841 cccatggtag cgtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg
      901 tgtgtgcaag cgcgcgcgac gtgtgtttta ggtaattttg ttgtgatgct ctgtccttaa
      961 actgcagcat gatacggaaa taggttattg aaaggtgtta ctttcaaatt gcacccaagg
     1021 aaggacacac tgtttatgcc ccaacaaaat ccactgtcta tcgtgggatt tacttagtca
     1081 gatatatgct taattctgtc ttagtgagtt ggaacatttt tcttggtatg tatagaccga
     1141 aatctattaa tttaatacag tttagaattt gtaggaaatt atattttttt ggtgagatgg
     1201 aattagcagt aaaaaaatca cacttataaa atgtgtttat ttttaattaa aattttcacc
     1261 atatttcctg taaaatatta ggagagatct gaaagcagcg taaacattgt aacaacttaa
     1321 acatctctag gatgtttttg tctaatgtat tttccatcta gcctgaatgt actgtaaatt
     1381 ttcatatgga cccacataac ataaaatttg tgacaaactt gcttatataa aacatgtgta
     1441 ttgaataaat ctcactatat tcctt
//