From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Alcaligenes eutrophus acetyl-coenzyme A synthetase (acoE) gene,
Date: 3 Jan 93 22:11:17 GMT
Distribution: bionet
Checksum: 48325 8

LOCUS       AFAACOSYN    3802 bp ds-DNA             BCT       23-DEC-1992
DEFINITION  Alcaligenes eutrophus acetyl-coenzyme A synthetase (acoE) gene,
            complete cds.
ACCESSION   M97217
KEYWORDS    acetyl-CoA synthetase; acetyl-coenzyme A synthetase.
SOURCE      Alcaligenes eutrophus (strain H16) DNA.
  ORGANISM  Alcaligenes eutrophus
            Prokaryotae; Gracilicutes; Scotobacteria; Aerobic rods and cocci;
            Alcaligenaceae.
REFERENCE   1  (bases 1 to 3802)
  AUTHORS   Priefert,H. and Steinbuechel,A.
  TITLE     Identification and molecular characterization of the acetyl
            coenzyme A synthetase gene (acoE) of Alcaligenes eutrophus
  JOURNAL   J. Bacteriol. 174, 6590-6599 (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     promoter        294..323
     terminator      2735..2768
     CDS             722..2704
                     /function="acetate and acetoin catabolism"
                     /gene="acoE"
                     /product="acetyl-coenzyme A synthetase"
                     /codon_start=1
                     /translation="MSAIESVMQEHRVFNPPEGFASQAAIPSMEAYQALCDEAERDYE
                     GFWARHARELLHWTKPFTKVLDQSNAPFYKWFEDGELNASYNCLDRNLQNGNADKVAI
                     VFEADDGSVTRVTYRELHGKVCRFANGLKALGIRKGDRVVIYMPMSVEGVVAMQACAR
                     LGATHSVVFGGFSAKSLQERLVDVGAVALITADEQMRGGKALPLKAIADDALALGGCE
                     AVRNVIVYRRTGGKVAWTEGRDRWMEDVSAGQPDTCEAEPVSAEHPLFVLYTSGSTGK
                     PKGVQHSTGGYLLWALMTMKWTFDIKPDDLFWCTADIGWVTGHTYIAYGPLAAGATQV
                     VFEGVPTYPNAGRFWDMIARHKVSIFYTAPTAIRSLIKAAEADEKIHPKQYDLSSLRL
                     LGTVGEPINPEAWMWYYKNIGNERCPIVDTFWQTETGGHMITPLPGATPLVPGSCTLP
                     LPGIMAAIVDETGHDVPNGNGGILVVKRPWPAMIRTIWGDPERFRKSYFPEELGGKLY
                     LAGDGSIRDKDTGYFTIMGRIDDVLNVSGHRMGTMEIESALVSNPLVAEAAVVGRPDD
                     MTGEAICAFVVLKRSRPTGEEAVKIATELRNWVGKEIGPIAKPKDIRFGDNLPKTRSG
                     KIMRRLLRSLAKGEEITQDTSTLENPAILEQLKQAQ"
     CDS             2807..>3802
                     /note="ORF 2"
                     /codon_start=1
                     /translation="MPEAQSRFRRRLLLYYGLFTLGLFGFVGMMGLLEKSNADALWLG
                     YVFLFITIAIYACIGLICRTSDLNEYYVASRRVPALFNGMAIAADWMSAASFIGLAGI
                     LFASGYEGLAYVMGWTGGYCLVAFLLAPYLRKYGGYTIPDFLAARYGNGKPGGNLPVR
                     AIAVLAASLCSFVYLVAQIQGVGLVVTRFIGVEFAVGIFFGLAGILVCSFLGGMRAVT
                     WTQVAQYIMMIVAFLVTVSMIAWKHHHEALPQLSYGTLLSQLDAREQQLEREPAEQAV
                     REYYRQQAILLQERIARLPDSFAQERDALDARLQDLRTRNAPLRDIKSLERERLEF"
BASE COUNT      672 a   1259 c   1259 g    612 t
ORIGIN      
        1 cccgggcgat attcagcacg gcgatgccgg tcatcgccag cagcaccgat gccaccgtgc
       61 gccgcgacag ccgttcgcgc agcagcagcc acgacaggat cgccaccgtg gccgggatgg
      121 tgctggtgat cacgccggcc gccatggcgc tggtcagccg cacgcccatt gagcatcagc
      181 agcgtaaaca ggaaggtgcc gaagaacgcc tgcaggaaca ggttcagcca ttccccgcgc
      241 gagacccggc gcatgcgcgc gggccggtac cagggcgcca ggcagacgat ggcaatgaca
      301 aaacgcagca gcgcgaacag cagcaccggc acatgcgcga tgatcgattt gccgaggccc
      361 acgttgctgc cgaccagtgc catcgaggca accaggaaga gggcgtagac cggggagaat
      421 gctgaagggt tggcgggcac gggtacggcc ttggtcttcc ggcgggcgcc gcggcgggtg
      481 ctgagccggc aggagcggca agggtaagcg ccgcgatcag gtatggaact cgcggcatta
      541 tagctgtgca tgcctaatgg agaaggctgc actttcgcgg cacgtgcgtg gatttttccc
      601 gctgcattgc aacaagttgc aggcacgccc cggcccaccg tcaggtctta ccgtcaggtt
      661 taagtaagcc gtcgggcgta acgtggcagc caaagccagt ccaaaatcag gagacaaacc
      721 tatgtccgcc atcgaatcgg tgatgcaaga gcatcgcgtg ttcaacccgc ccgaaggctt
      781 cgccagccag gccgcgatcc ccagcatgga ggcctaccag gcgctgtgcg acgaagccga
      841 gcgtgactat gaaggtttct gggcgcgcca cgcgcgcgag ctgctgcact ggaccaagcc
      901 cttcaccaag gtgctggacc aaagcaacgc accgttctac aagtggttcg aagacggcga
      961 gctcaacgcc tcttacaact gcctggaccg caatctgcag aacggcaatg cggacaaggt
     1021 cgcgatcgtg ttcgaggccg acgacggcag cgtgacgcgc gtcacctacc gcgagctgca
     1081 tggcaaggtg tgccgcttcg ccaacggcct gaaggcgctc ggcatcagga agggcgaccg
     1141 cgtggtgatc tacatgccga tgtcggtcga aggcgtggtc gcgatgcagg cctgcgcacg
     1201 cctgggcgcc acgcactcgg tggtgttcgg cggcttctcg gccaagtcgc tgcaggagcg
     1261 gctggtggac gtgggcgcgg tggcgctgat caccgccgac gagcagatgc gcggcggcaa
     1321 ggcgctgccg ctcaaggcca tcgccgatga cgcgctggcg ctgggcggct gcgaggccgt
     1381 caggaacgtg atcgtctacc gccgcaccgg cggcaaggtt gcctggaccg aaggccgcga
     1441 ccgctggatg gaagatgtca gcgccggcca gccggatacc tgcgaagccg agccggtgag
     1501 cgccgagcac ccgctgttcg tgctctacac ctccggctcc accggcaagc ccaagggcgt
     1561 gcagcacagc accggcggct acctgctgtg ggcgctgatg acaatgaagt ggaccttcga
     1621 catcaagccc gacgacctgt tctggtgtac cgcggacatc ggctgggtca ccggccacac
     1681 ctatattgcc tacggcccgc tggccgcggg cgccacccag gtggtgttcg aaggcgtgcc
     1741 gacctacccc aacgccggcc gcttctggga catgatcgcg cgccacaagg tcagcatctt
     1801 ctacaccgcg ccgaccgcga tccgctcgct gatcaaggcc gccgaggccg acgagaagat
     1861 ccacccgaaa cagtacgacc tgtccagcct gcgcctgctc ggcaccgtgg gcgagccgat
     1921 caaccccgaa gcctggatgt ggtactacaa gaacatcggc aacgagcgct gcccgatcgt
     1981 cgacaccttc tggcagaccg agaccggcgg ccacatgatc acgccgctgc cgggcgcgac
     2041 gccgctggtg ccgggttcgt gcacgctgcc gctgccgggc atcatggccg ccatcgtcga
     2101 cgagaccggc catgacgtgc ccaacggcaa cggcggcatc ctggtggtca agcgtccgtg
     2161 gccggccatg atccgcacca tctggggcga tccggagcgc ttcaggaaga gctacttccc
     2221 cgaagagctc ggcggcaagc tctacctggc cggcgacggc tcgatccgcg acaaggacac
     2281 cggctacttc accatcatgg gccgcatcga cgacgtgctg aacgtgtcgg gccaccgcat
     2341 ggggacgatg gagatcgagt ccgcgctggt gtccaacccg ctggtggctg aagccgccgt
     2401 ggtgggccgc cccgacgaca tgaccggcga ggccatctgc gccttcgtcg tgctcaagcg
     2461 ttcgcgtccg actggcgaag aggccgtcaa gatcgcgacg gagctgcgca actgggtcgg
     2521 caaggagatc ggcccgatcg ccaagcccaa ggacatccgc tttggcgaca acctgcccaa
     2581 gacgcgctcg ggcaagatca tgcggcgcct gctgcggtcg ctggccaagg gggaggagat
     2641 cacgcaggac acctcgacgc tggagaatcc ggccatcctg gagcagctca agcaggcgca
     2701 gtgatcgtct ggcttcggcc cgatcgagcc cttgaacccg ccgccgcgtt cccgcgcgcc
     2761 ggcgggtttg ttacgatagc cccccaccga ccacggaccg tccccaatgc ctgaagccca
     2821 gtcgcgcttt cgccgacggt tgctgctgta ctacggcctg ttcacgctag ggttgttcgg
     2881 cttcgtcggc atgatggggc tgcttgaaaa gtccaatgcc gatgcgctgt ggctcggcta
     2941 cgtcttcctc ttcatcacca tcgcgatcta tgcctgcatc ggcctgatct gccgcacctc
     3001 tgacctgaac gagtactacg ttgcctcgcg gcgcgtgccg gcgctgttca acggcatggc
     3061 gattgccgcc gactggatga gcgcggcgtc cttcatcggc ctggccggca tcctgtttgc
     3121 ctcgggctac gaagggctgg cctatgtgat gggctggacc ggcggctact gcctggtggc
     3181 cttcctgctg gcgccgtacc tacgcaagta cggcggctac accattcccg atttcctcgc
     3241 ggcgcgctac ggcaacggca agcccggcgg caacctgcca gtgcgcgcga tcgcggtgct
     3301 ggcggcctcg ctctgctcct tcgtctacct ggttgcgcag atccagggcg tggggctggt
     3361 ggtgacgcgc tttatcggcg tggagtttgc cgtcggcatc ttcttcgggc tggccggcat
     3421 cctggtgtgc tcgttcctgg gcggcatgcg cgcggtcaca tggacgcagg tggcgcagta
     3481 catcatgatg atcgtcgcct tcctggtcac ggtatcgatg atcgcgtgga agcatcacca
     3541 cgaagcgttg ccgcagctca gctacggcac gctgctgtcg caactcgacg cgcgcgagca
     3601 gcagctggag cgcgagccgg ccgagcaggc cgtgcgcgag tactaccggc agcaggccat
     3661 cctgctgcag gagcgcatcg cccggctgcc cgattccttt gcccaggagc gtgacgcgct
     3721 cgacgcgcgg ctgcaggacc tgcgcacgcg caatgccccg ctgcgcgaca tcaagtcgct
     3781 ggagcgcgaa cggctggaat tc
//