From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Salmonella typhimurium oxaloacetate decaroxylase (oad[G,A,B])
Date: 28 Dec 92 21:46:25 GMT
Distribution: bionet
Checksum: 05832 9

LOCUS       STYOADGABA   4053 bp ds-DNA             BCT       02-DEC-1992
DEFINITION  Salmonella typhimurium oxaloacetate decaroxylase (oad[G,A,B])
            genes, complete cds.
ACCESSION   M96434
KEYWORDS    membrane protein; oxaloacetate decarboxylase; sodium pump.
SOURCE      Salmonella typhimurium (strain LT2) DNA.
  ORGANISM  Salmonella typhimurium
            Prokaryotae; Gram-negative facultatively anaerobic rods;
            Enterobacteriaceae.
REFERENCE   1  (bases 1 to 4053)
  AUTHORS   Woehlke,G., Wifling,K. and Dimroth,P.
  TITLE     Sequence of the sodium ion pump oxaloacetate decarboxylase from
            Salmonella typhimurium
  JOURNAL   J. Biol. Chem. 267, 22798-22803 (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     misc_feature    3841..3854
                     /note="This feature applies to a gene which lacks a coding
                     region feature. "
                     /gene="oad"
     RBS             430..433
                     /gene="oadG"
     RBS             697..701
                     /gene="oadA"
     RBS             2484..2487
                     /gene="oadB"
     terminator      3841..3854
                     /gene="oad"
     CDS             440..691
                     /EC_number="4.1.1.3"
                     /gene="oadG"
                     /product="oxaloacetate decarboxylase"
                     /codon_start=1
                     /translation="MTNAALLLGEGFTLMFLGMGFVLAFLFLLIFAIRGMSAAVNRFF
                     PEPAPAPNAPAAAAPVVDDFTRLKPVIAAAIHHHHRLNA"
     CDS             707..2482
                     /EC_number="4.1.1.3"
                     /gene="oadA"
                     /product="oxaloacetate decarboxylase"
                     /codon_start=1
                     /translation="MTIAITDVVLRDAHQSLFATRLRLDDMLPIAAALDDVGYGSLEC
                     WGGATFDACIRFLGEDPWLRLRELKKAMPKTPLQMLLRGQNLLGYRHYADDVVERFVE
                     RAVKNGMDVFRVFDAMNDPRNMKAALQAVRSHGAHAQGTLSYTTSPAHTLQTWLDLTE
                     QLLETGVDSIAIKDMSGILTPMAAYELVSEIKKRFEVRLHLHCHATTGMAEMALLKAI
                     EAGVDGVDTAISSMSATYGHPATEALVATLAGTEHDTGLDILKLENIAAYFREVRKKY
                     HAFEGQLKGYDSRILVAQVPGGMLTNLESQLKQQNAADKLDQVLAEIPRVREDLGFIP
                     LVTPTSQIVGTQAVLNVLTGERYKTIAKETAGILKGEYGHTPVPVNAALQARVLEGGA
                     PVTCRPADLLKPELAELEADVRRQAQEKGITLAGNAIDDVLTVALFPQIGLKFLENRN
                     NPAAFEPLPQAEAAQPVAKAEKPAASGIYTVEVEGKAFVVKVSDGGDISQLTAAVPAA
                     SSAPVQAAAPAGAGTPVTAPLAGNIWKVIATEGQTVAEGDVLLILEAMKMETEIRAAQ
                     AGTVRGIAVKSGDAVSVGDTLMTLA"
     CDS             2495..3796
                     /EC_number="4.1.1.3"
                     /gene="oadB"
                     /product="oxaloacetate decarboxylase"
                     /codon_start=1
                     /translation="MESLNALLQGMGLMHLGAGQAIMLLVSLLLLWLAIAKKFEPLLL
                     LPIGFGGLLSNIPEAGLALTALESLLAHHDAGQLAVIAAKLHCAPDVHAIKEALALAL
                     PSVQNQMENLAVDMGYTPGVLALFYKVAIGSGVAPLVIFMGVGAMTDFGPLLANPRTL
                     LLGAAAQFGIFATVLGALTLNYFGLISFTLPQAAAIGIIGGADGPTAIYLSGKLAPEL
                     LGAIAVAAYSYMALVPLIQPPIMKALTSETERKIRMVQLRTVSKREKILFPVVLLMLV
                     ALLLPDAAPLLGMFCFGNLMRESGVVERLSDTVQNGLINIVTIFLGLSVGAKLVADKF
                     LQPQTLGILLLGVIAFGIGTAAGVLMAKLLNLCSKNKINPLIGSAGVSAVPMAARVSN
                     KVGLESDAQNFLLMHAMGPNVAGVIGSAIAAGVMLKYVLAM"
BASE COUNT      799 a   1180 c   1286 g    788 t
ORIGIN      
        1 ggtaccgaag agggctgtgc tgaacacaaa gcgctgcact gcgtattccc tgccggttgt
       61 gcggttgtag ccgcgtgtgc gtggaagaaa ttgaagatgc ccaatggcgc gatctgggga
      121 tgccggaaac gtctgtgggt ctgtcgggtt aaagagtttg gccgctgatt gtttctattg
      181 atacccatgg gaataatctg tttgaacaga acaaaattat ttttaatcag cgtaaagaga
      241 tcgtcgctga tgaaatatgc cagaacgtaa gtttcatcaa ataaacctga ttacgtaaat
      301 tgcacgataa tggtggtaat acgttatgac gataataata accaagggct ctggccgggg
      361 cggctcaggg aaatccaccc ttattatttc atcgtcataa atagccccat tatcgtgcct
      421 cctgcctgaa ggctatttta tgactaatgc agctctgcta ttaggtgaag gcttcaccct
      481 gatgttcctc ggcatggggt tcgtgctggc gtttctgttc ctgctgattt tcgccatccg
      541 cggcatgtcc gccgccgtca accgcttctt ccctgaaccg gctccggcgc cgaacgcacc
      601 tgccgccgcc gcgcccgtcg tggacgactt cacccgctta aagccggtca ttgccgccgc
      661 tatccaccac catcaccgcc ttaatgctta attcccggag ggatccatga ccattgccat
      721 taccgatgtc gtcctgcgtg acgctcacca gtctctgttc gccacccgcc tgcgccttga
      781 tgacatgctg ccgattgccg ccgcgctcga tgacgtgggc tacgggtcgc tggagtgctg
      841 gggcggcgcc acctttgacg cctgtatccg ctttctcggc gaagacccgt ggctgcgcct
      901 gcgcgaactc aaaaaagcca tgcccaaaac cccgctgcag atgttgctgc gcggccagaa
      961 cctgctcggc taccgccact acgccgatga cgtggtggag cgcttcgtcg aacgcgcggt
     1021 gaaaaacggc atggatgtgt tccgcgtctt cgatgccatg aacgacccgc gcaatatgaa
     1081 agccgcatta caggcggtgc gcagccacgg cgcgcacgcc caggggaccc tgagttacac
     1141 caccagcccg gcgcacaccc tgcagacctg gctggattta acggagcaac tgctggaaac
     1201 cggcgttgac tcgattgcca tcaaggatat gtccggcatt ctcacaccga tggcggcgta
     1261 tgagctggtc agcgaaatca aaaaacgttt tgaggtgcgc ctgcacctgc actgtcacgc
     1321 caccaccggg atggcggaga tggccctgct gaaggctatc gaggcgggcg tcgacggcgt
     1381 ggacacggcg atttcctcca tgagcgccac ctacggccac ccggccaccg aagcgctggt
     1441 ggcaacgctt gccggcacgg aacacgacac cgggctggat atcctgaagc tggaaaatat
     1501 cgccgcgtac ttccgcgagg tgcgcaaaaa gtatcacgcc tttgaaggcc agctgaaggg
     1561 ctacgacagc cgtattctgg tggcccaggt accgggcgga atgctcacca acctcgaaag
     1621 ccagctgaag cagcagaacg cggcggacaa actcgaccag gtgctggcgg aaatcccccg
     1681 cgtgcgcgag gacctcggct ttatcccgct ggtgacccca acctcgcaga ttgtcggcac
     1741 ccaggcggtg ctcaacgtcc tgaccggcga gcgctacaaa accattgcca aagaaacggc
     1801 gggcattctg aaaggcgaat acggccacac cccggtgccg gtgaacgccg cgttacaggc
     1861 ccgcgtgctg gaagggggcg ccccggtgac ctgccgtccg gcggatttac tcaaaccgga
     1921 gctggctgaa ctggaagcgg acgtcaggcg ccaggcgcag gagaagggga ttactctggc
     1981 gggaaacgcc atcgacgacg tgctcaccgt ggcgctgttc ccgcaaatcg gcctcaaatt
     2041 ccttgagaac cgcaataacc cggcggcgtt tgagccactg ccgcaggcgg aagccgcgca
     2101 gccggtggca aaagcagaga agcctgccgc ctccggtatc tacaccgtgg aagtggaagg
     2161 caaagccttt gtggtgaagg tcagcgacgg cggcgatatc agccagctca ctgcggctgt
     2221 tccggcggcc tcttccgcac cggttcaggc tgccgccccg gcaggcgccg gcaccccggt
     2281 caccgcgccg ctggcgggga atatctggaa ggtgattgcc accgagggcc agacggtggc
     2341 cgaaggcgac gtgctgctga ttctggaagc catgaagatg gaaaccgaaa tccgcgccgc
     2401 gcaggccggg acggtacgcg gtatcgcggt gaagtccggg gacgcggtct ccgtcggcga
     2461 caccctgatg acgctggcgt aacggagaac tgaaatggaa agtctgaacg ccctgcttca
     2521 gggcatgggg ctgatgcacc ttggcgcagg ccaggccatc atgctgctgg tcagcctgct
     2581 gctgctgtgg ctggcgattg cgaagaagtt cgagccgtta ctgctgctgc cgataggctt
     2641 cggcgggctg ctgtcgaaca ttccggaggc cgggctggcg ctgaccgcgc tggaaagcct
     2701 gctggcgcac cacgacgccg gacagctggc ggtgattgcc gcgaagctcc actgcgcgcc
     2761 ggacgtgcat gccattaagg aagcattagc gctggcgctg ccctccgtcc agaaccagat
     2821 ggaaaatctg gcggtggaca tgggctacac gccgggggtg ctggcgctgt tctacaaagt
     2881 ggcgattggc tccggtgtcg cgccgctggt catcttcatg ggcgtcggcg cgatgaccga
     2941 cttcggcccg ctgctggcca acccgcgcac cctgctgctc ggcgcggcgg cgcagttcgg
     3001 tatcttcgcc accgtgctgg gggcgctgac gctgaactac ttcggcctta tcagcttcac
     3061 cctgccgcag gcggcggcca tcggcattat cggcggcgcg gacggcccga cggcgattta
     3121 tctgtcgggc aagctggcgc cggagctgct gggggccatc gcggtggcgg cgtactcgta
     3181 tatggcgctg gtgccgttaa tccagccgcc gattatgaag gcgctgacca gcgagacgga
     3241 gcggaaaatc cgcatggtgc agctgcgcac ggtgagtaag cgggaaaaaa tcctcttccc
     3301 ggtggtgctg ctcatgctgg tggccctgct gctgccggac gcggcgccgc tgctggggat
     3361 gttctgtttt ggcaacctga tgcgcgaaag cggcgtggtg gagcgtctga gcgacacggt
     3421 acagaacggg ctgattaata tcgtcaccat cttcctcggg ctgtcggtgg gcgcgaagct
     3481 ggtcgcggac aagttcctgc agccgcagac gctgggcatt ctgctgctgg gtgtgattgc
     3541 gttcgggatt gggacggcgg ccggggtgct gatggcgaag ctgctgaacc tgtgcagtaa
     3601 gaacaaaatc aacccgctta tcggttcggc gggggtgtcg gcggtgccga tggcggcacg
     3661 cgtatcgaac aaagtgggcc tggaatcaga cgcgcagaac ttcctgctga tgcacgcgat
     3721 ggggccgaac gtggccgggg tgattggctc ggccattgcg gcgggcgtga tgctcaagta
     3781 cgtgctggcg atgtaatcca atctaatcgg gagcagtgat tctgttcccg caaaatgagg
     3841 acacagtaat gagtgaaatg gttgcgtttc ggcaaggtac atccatgcca tccagagaaa
     3901 ccatcttgca ttatgttgtt gagacggtga atcagataac tgagcttgag ccagcgctgc
     3961 atttactgcc gtggtctggc gtcaactcag caatatatga gcaacgtttt gcacagtgtt
     4021 acgacgaggg gctttgtgcc gcacagacgt cgg
//