From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human (clones SW11-1 and cKi-ras2-76) cellular c-Ki-ras2
Date: 31 Jul 92 10:01:34 GMT
Distribution: bionet
Checksum: 39955     5

LOCUS       HUMRASK02     290 bp ds-DNA             PRI       31-JUL-1992
DEFINITION  Human (clones SW11-1 and cKi-ras2-76) cellular c-Ki-ras2
            proto-oncogene, exon 1.
ACCESSION   L00045 K00652 K00653
KEYWORDS    K-ras oncogene; c-myc oncogene; proto-oncogene; ras oncogene;
            transforming gene; viral homology.
SEGMENT     2 of 6
SOURCE      Homo sapiens (library: Lawn et al.) lung DNA; and Homo sapiens
            colon and lung cDNA to mRNA.
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 96 to 206)
  AUTHORS   Shimizu,K., Birnbaum,D., Ruley,M.A., Fasano,O., Suard,Y.,
            Edlund,L., Taparowsky,E., Goldfarb,M. and Wigler,M.
  TITLE     Structure of the Ki-ras gene of the human lung carcinoma cell line
            Calu-1
  JOURNAL   Nature 304, 497-500 (1983)
  STANDARD  full staff_review
REFERENCE   2  (bases 1 to 290)
  AUTHORS   McGrath,J.P., Capon,D.J., Smith,D.H., Chen,E.Y., Seeburg,P.H.,
            Goeddel,D.V. and Levinson,A.D.
  TITLE     Structure and organization of the human Ki-ras proto-oncogene and a
            related processed pseudogene
  JOURNAL   Nature 304, 501-506 (1983)
  STANDARD  full staff_review
REFERENCE   3  (bases 96 to 206)
  AUTHORS   Capon,D.J., Seeburg,P.H., McGrath,J.P., Hayflick,J.S., Edman,U.,
            Levinson,A.D. and Goeddel,D.V.
  TITLE     Activation of Ki-ras2 gene in human colon and lung carcinomas by
            two different point mutations
  JOURNAL   Nature 304, 507-513 (1983)
  STANDARD  full staff_review
REFERENCE   4  (bases 96 to 206)
  AUTHORS   McCoy,M.S., Bargmann,C.I. and Weinberg,R.A.
  TITLE     Human colon carcinoma Ki-ras2 oncogene and its corresponding
            proto-oncogene
  JOURNAL   Mol. Cell. Biol. 4, 1577-1582 (1984)
  STANDARD  full staff_review
REFERENCE   5  (bases 96 to 206)
  AUTHORS   Nakano,H., Yamamoto,F., Neville,C., Evans,D., Mizuno,T. and
            Perucho,M.
  TITLE     Isolation of transforming sequences of two human lung carcinomas:
            Structural and functional analysis of the activated c-K-ras
            oncogenes
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 81, 71-75 (1984)
  STANDARD  full staff_review
COMMENT     Codon 12 at base 129 encodes glycine (ggt) in the normal c-Ki-ras2
            gene product, valine (gtt) in SW480 cells, and cysteine (tgt) in
            Calu-1 and PR371 cells. This is the only distinguishing feature
            among the three coding segments.
            See segment 1 of this entry for more comments.
            Human DNA (genomic library of Lawn et al.) [2]; human cDNA to colon
            (SW480) and lung (Calu-1) carcinoma cell lines mRNA, multiple
            clones [3]; human lung carcinoma cell line Calu-1 DNA [1]; human
            lung carcinoma cell lines PR310 and PR371 DNA [5]; human colon
            carcinoma cell line SW480 cDNA to mRNA, clone SW11-1, and human
            cell line GM637 cDNA to mRNA, clone cKi-ras2-76 [4].
FEATURES             Location/Qualifiers
     intron          order(L00044:580..>741,<1..84)
                     /number=A
                     /note="5.5 kb gap"
                     /gene="c-Ki-ras2"
     exon            85..206
                     /number=1
                     /type="nontransforming cells"
                     /gene="c-Ki-ras2"
     mutation        replace(129,"t")
                     /type="Calu-1 and PR371 cells"
                     /gene="c-Ki-ras2"
     mutation        replace(130,"t")
                     /type="SW480 cells"
                     /gene="c-Ki-ras2"
     conflict        replace(187
                     /citation=[1]
BASE COUNT       84 a     40 c     63 g    103 t
ORIGIN
        1 gtactggtgg agtatttgat agtgtattaa ccttatgtgt gacatgttct aatatagtca
       61 cattttcatt atttttatta taaggcctgc tgaaaatgac tgaatataaa cttgtggtag
      121 ttggagctgg tggcgtaggc aagagtgcct tgacgataca gctaattcag aatcattttg
      181 tggacgaata tgatccaaca atagaggtaa atcttgtttt aatatgcata ttactggtgc
      241 aggaccattc tttgatacag ataaaggttt ctctgaccat tttcatgagt
//