From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Eubacterium sp. (OS type C) 16S ribosomal RNA, partial sequence.
Date: 6 Nov 92 18:27:57 GMT
Distribution: bionet
Checksum: 57757 5

LOCUS       EUBRRDAAB    1138 bp ss-rRNA            RNA       02-NOV-1992
DEFINITION  Eubacterium sp. (OS type C) 16S ribosomal RNA, partial sequence.
ACCESSION   M62775 X52546
KEYWORDS    16S ribosomal RNA.
SOURCE      Cyanobacterium sp., cDNA to rRNA.
  ORGANISM  Eubacterium sp.
            Prokaryotae; Eubacteria.
REFERENCE   1  (bases 1 to 1138)
  AUTHORS   Ward,D.M., Weller,R. and Bateson,M.M.
  TITLE     16S rRNA sequences reveal numerous uncultured microorganisms in a
            natural community
  JOURNAL   Nature 345, 63-65 (1990)
  STANDARD  full automatic
REFERENCE   2  (bases 1 to 1138)
  AUTHORS   Weller,R., Weller,J.W. and Ward,D.M.
  TITLE     16S rRNA sequences of uncultivated hot spring cyanobacterial mat
            inhabitants retrieved as randomly primed cDNA
  JOURNAL   Appl. Environ. Microbiol. 57, 1146-1151 (1991)
  STANDARD  full automatic
REFERENCE   3  (bases 1 to 1138)
  AUTHORS   Ward,D.M., Bateson,M.M., Weller,R. and Ruff-Roberts,A.L.
  TITLE     Ribosomal RNA analysis of microorganisms as they occur in nature
  JOURNAL   Adv. Microb. Ecol. 12, 219-286 (1992)
  STANDARD  full automatic
REFERENCE   4  (bases 1 to 1138)
  AUTHORS   Ward,D.M., Kopczynski,E.D., Heimbuch,B.K., Bateson,M.M. and
            Weller,R.
  TITLE     Uncultivated cyanobacteria, Chloroflexus-like and Spirochete-like
            inhabitants of a hot spring microbial mat
  JOURNAL   Appl. Environ. Microbiol. (1992) In press
  STANDARD  full automatic
COMMENT     Unknown Eubacterium from Octopus Spring cyanobacterial mat,
            Yellowstone National Park: clones OSII-23; OSIII-1,15; OSV-L-8;
            OSV-S-1,14,17; OSVI-L-11.
               [1] reports bases 752-1138; [3] reports bases 1-1138; [4]
            reports bases 1-1138; [2] reports bases 1-1138.
FEATURES             Location/Qualifiers
     rRNA            <1..>1138
                     /note="aligns with E. coli 16S rRNA positions 236-1392"
                     /product="16S ribosomal RNA"
                     /citation=[2]
                     /citation=[1]
                     /citation=[4]
                     /citation=[3]
     unsure          212..249
                     /note="these base pairs may or may not be present"
                     /citation=[2]
                     /citation=[1]
                     /citation=[4]
                     /citation=[3]
     unsure          257
                     /note="this base pair may or may not be present"
                     /citation=[2]
                     /citation=[1]
                     /citation=[4]
                     /citation=[3]
     unsure          265
                     /note="this base pair may or may not be present"
                     /citation=[2]
                     /citation=[1]
                     /citation=[4]
                     /citation=[3]
     variation       replace(1024,"t")
                     /note="in clone OSV-L-8"
                     /citation=[2]
                     /citation=[1]
                     /citation=[4]
                     /citation=[3]
     variation       replace(1024,"c")
                     /note="in clones OSII-23, OSIII-15, and OSV-S-1"
                     /citation=[2]
                     /citation=[1]
                     /citation=[4]
                     /citation=[3]
BASE COUNT      232 a    305 c    377 g    173 t     51 others
ORIGIN      
        1 gcggcccatc aggtcgttgg tggggtaagg gcctaccaag ccgatgacgg gtagctggtc
       61 tgggaggatg accagccaga ctgggactga gacacggccc agactcctac gggaggcagc
      121 agcaaggaat tttcggcaat gggcgcaagc ctgaccgagc aacgccgcgt gcgggatgac
      181 ggccttcggg ttgtaaaccg cttttcgggg gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      241 nnnnnnnnng taccccggaa tcagccccgg ctaactctgt gccagcagcc gcggtaagac
      301 agagggggng agcgttgtcc ggagtcactg ggcgtaaagc gcgcgcaggc ggcaacctca
      361 gtgtcgtgtg aaagcccccg gctcaaccgg gggaggccat ggcaaactgg gtcgctcgag
      421 ctgyggagag gcccctcgaa ttgccggtgt agcggtgaaa tgcgtagaga tcggcaggaa
      481 gaccaagggg gaagccaggg ggctggccgc asstgacgct gaggcgcgac agcgtgggga
      541 gcaaaccgga ttagataccc gggtagtcca cgccgtaaac gatgaccact cggcgtgtgg
      601 cgactattga cgtcgcggng ngccctagct cacgcgataa gtggtccgcc tgggaactac
      661 gagcncaass ttaaaactca aaggaattga cgggggcccg cacaagcagc ggagnntgtg
      721 gtttaattcg acacaacccg cagaacctta cccagactgg acatgacggt gcagacggcg
      781 gaaacgtcgt cgcctgcgag ggtccgtcac aggtgctgca tggctgtcgt cagctcgtgt
      841 cgtgagatgt tgggttaagt cccgcaacga gcgcaacccc tgcggttagt tacccgtgtc
      901 taaccggact gcccttcagg gaggaaggcg gggatgacgt caagtccgca tggcccttac
      961 gtctggggcg acacacacgc tacaatggcg ccgacaatgc gtcgctcccg cgcaagcgga
     1021 tgcyaatcgc caaacggngc cccagtgcag atcgggggct gcaactcgcc cccgtgaagg
     1081 cggagttgct agtaaccgcg tatcagccat ggcgcggtga atacgtaccc gggccttg
//