From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Pseudomonas aeruginosa methylmalonate semialdehyde dehydrogenase
Date: 22 Jul 92 10:03:27 GMT
Distribution: bionet
Checksum: 08694    13

LOCUS       PSEMMSRAB    5417 bp ds-DNA             BCT       22-JUL-1992
DEFINITION  Pseudomonas aeruginosa methylmalonate semialdehyde dehydrogenase
            (mmsA) gene complete cds, 3-hydroxyisobutyrate dehydrogenase (mmsB)
            gene complete cds, ORF1 5'end.
ACCESSION   M84911
KEYWORDS    3-hydroxyisobutyrate dehydrogenase; acetyl-CoA synthase-like;
            methylmalonate semialdehyde dehydrogenase; mmsA gene; mmsAB operon;
            mmsB gene; mmsR gene; transcriptional regulator; valine metabolism.
SOURCE      Pseudomonas aeruginosa DNA.
  ORGANISM  Pseudomonas aeruginosa
            Prokaryotae; Gracilicutes; Scotobacteria; Pseudomonadaceae.
REFERENCE   1  (bases 1 to 5417)
  AUTHORS   Steele,M.I., Lorenz,D., Hatter,K., Park,A. and Sokatch,J.R.
  TITLE     Characterization of the mmsAB operon of Pseudomonas aeruginosa PAO
            encoding methylmalonate semialdehyde dehydrogenase and
            3-hydroxyisobutyrate dehydrogenase
  JOURNAL   J. Biol. Chem. 267, 13585-13592 (1992)
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     CDS             complement(463..1386)
                     /function="regulatory gene for mmsAB operon"
                     /note="member of the AraC/XylS family of transcriptional
                     regulators"
                     /gene="mmsR"
                     /evidence=EXPERIMENTAL
                     /codon_start=1
                     /translation="MDYIAEPATDMSHTSDWPLPADSLRLPIPAALLEKLAVHPLSRD
                     LYPTSLGHYRRARDHRMSRERHDEHLLIYCSEGQGLLRVREGEAWREYRVGSGDLLWL
                     PPGMAHDYAADDRQPWTIFWTHLRGDTATWLQHSAGYRDTPLRHWGLQHALLGGFEQL
                     LEVRHSGYRFQHFLLAASRLRSLLCQLPLLRPLREGSLDLDGLHAYMREHLHARLELE
                     RLAAFCNLSKFHFVSRYKAITGRTPIQHFLHLKIEYACQLLDSSDQSVARVGQAVGYD
                     DSYYFSRLFSKVMGLSPSAYRQRVRQGEGGA"
                     /citation=[1]
     mRNA            1441..3923
                     /note="joint mRNA encoding mmsA and mmsB gene"
                     /gene="mmsAB"
                     /evidence=EXPERIMENTAL
                     /citation=[1]
     RBS             1509..1520
                     /gene="mmsA"
                     /note="putative"
                     /citation=[1]
     CDS             1518..3011
                     /function="catalyzes step in distal pathway of
                     valinemetabolism, located on a operon with gene
                     for3-hydroxyisobutyrate dehydrogenase"
                     /EC_number="1.2.1.27"
                     /product="methylmalonate semialdehyde dehydrogenase"
                     /gene="mmsA"
                     /evidence=EXPERIMENTAL
                     /codon_start=1
                     /translation="MSVPVRHLIAGAFVEGLGAQRIPVSNPLDNSTLAEIACASAEQV
                     EQAVASARETFASWKETPVSERARVMLRYQALLKEHHDELAKIVSSELGKTFEDAKGD
                     VWRGIEVVEHACNVPSLLMGETVENVARNIDTYSITQPLGVCVGITPFNFPAMIPLWM
                     FPLAIACGNAFILKPSEQVPLTSVRLAELFLEAGAPKGVLQVVHGGKEQVDQLLKHPQ
                     VKAVSFVGSVAVGQYVYHTGTAHNKRVQSFAGAKNHMVIMPDADKAQVISNLVGASVG
                     AAGQRCMAISVAVLVGAAREWIPEIRDALAKVRPGPWDDSGASYGPVINPQAKARIER
                     LIGQGVEEGAQLLLDGRGYKVEGYPDGNWVGPTLFAGVRPDMAIYREEVFGPVLCLAE
                     VDSLEQAIRLINESPYGNGTSIFTSSGAAARTFQHHIEVGQVGINIPIPVPLPFFSFT
                     GWKGSFYGDLHAYGKQGVRFYTETKTVTARWFDSDSVAGTNFSIQMR"
                     /citation=[1]
     mat_peptide     1521..3008
                     /product="methylmalonate semialdehyde dehydrogenase"
                     /gene="mmsA"
                     /evidence=EXPERIMENTAL
                     /codon_start=1
                     /citation=[1]
     RBS             3015..3029
                     /gene="mmsB"
                     /note="putative"
                     /citation=[1]
     CDS             3027..3923
                     /function="catalyzes step in distal valine metabolic
                     pathway, located on an operon with methylmalonate
                     semialdehyde dehydrogenase"
                     /EC_number="1.1.1.31"
                     /product="3-hydroxyisobutyrate dehydrogenase"
                     /gene="mmsB"
                     /evidence=EXPERIMENTAL
                     /codon_start=1
                     /translation="MTDIAFLGLGNMGGPMAANLLKAGHRVNVFDLQPKAVLGLVEQG
                     AQGADSALQCCEGAEVVISMLPAGQHVESLYLGDDGLLARVAGKPLLIDCSTIAPETA
                     RKVAEAAAAKGLTLLDAPVSGGVGGARAGTLSFIVGGPAEGFARARPVLENMGRNIFH
                     AGDHGAGQVAKICNNMLLGILMAGTAEALALGVKNGLDPAVLSEVMKQSSGGNWALNL
                     YNPWPGVMPQAPASNGYAGGFQVRLMNKDLGLALANAQAVQASTPLGALARNLFSLHA
                     QADAEHEGLDFSSIQKLYRGKD"
                     /citation=[1]
     CDS             4025..5417
                     /note="ORF located downstream of mmsAB operon, has
                     sequence similarity to an acetyl-CoA synthetase"
                     /note="ORF1"
                     /note="putative"
                     /codon_start=1
                     /translation="MTYLQQHARSIAEPAAFWAEQARSLAWYQAPANILESLPDGTHR
                     WFADGRLNSAYLALDRQIEEGRGEQTALIYDSPVTGTQDRYSYLRLRDEVARLAGALR
                     ALGVGKGDRVIIYMPMVPQAAMAMLACARLGAVHSVVFGGFAPYELALRIDDATPKLV
                     LTASCGLEFDRVIEYKPLVDKALELAIHQPAHVMVWQRPQAPARLHPGRDLDWQDCLA
                     AAEPADPLPVASGDPLYIMYTSGTTGKPKGIVRDNGGHAVAVRYAVRTIYGMQAGDVW
                     WGISDVGWVVGHSLIVYGPLMCGCTTVFYEGKPVRTPDAGAYWRVIEEHRVNSLFCAP
                     TAIRAIRKEDPHGERVKRYDLGSLRHLFLAGEKLDSSTQHWLEEHTGRPVHDHWWQTE
                     TGWPVTAPCIGIAGHDLRAGSTNRAVPGYHVQVLDDEGRPLGANRQGAIVIALPLPPG
                     CAQTLWGDHPRYLQ"
                     /citation=[1]
                     /partial
BASE COUNT      881 a   1832 c   1880 g    824 t
ORIGIN
        1 ctgcagttca gcgtggcggc gggcgcttcg gcgctggtcg gcgtgctcca cgacggcacg
       61 gcgatcccca tgactctggt gatcgccgtc tgcggcgcgg tcgcgaccct gctcgcctgg
      121 cagaccgggc gcctggagcg ccaggccgcc tgagcgcgga tcaggccgtt cgctgtttca
      181 gttcgggcag cacatggggc gcgcgcaggc ggccctcgag gcggtcgacg aaacgccggg
      241 cctcgctttc gctacggaag ttgacggcct gctggtcgag gcagacttgc cagcattggc
      301 ctttcggggt ttgcgacggg cggacgagga tgttcatggc gattcatcca tcggggctgg
      361 aaagggagct tcagtctagt ctgtcggccc ggaaaacact gaccgggatc agtttgccga
      421 caactggtcg ccgcttcgtt tacagccccg ccgcagagcg cttcaggctc ctccctcgcc
      481 ctgccttacc cgctgccggt aggcgctcgg cgacaaaccc atgaccttgc tgaacaggcg
      541 cgagaagtag tagctgtcgt cgtagccgac cgcctggccg acccgggcca cgctctggtc
      601 gctgctgtcc agcaactggc aggcgtactc gatcttcagg tgcaggaagt gctggatagg
      661 cgtacggccg gtgatcgcct tgtagcgact gacgaaatgg aacttcgaca ggttgcagaa
      721 ggccgccagg cgttccagtt cgaggcgcgc atgcaggtgt tcgcgcatgt aggcgtgcag
      781 cccgtcgagg tccaggctgc cctcgcgcag cgggcgcagc agcggtagct ggcagagcag
      841 gctgcgcagg cggctggcgg cgagcaggaa gtgctggaag cggtagccgc tgtggcgcac
      901 ctccagcagt tgctcgaagc cgccgagcag ggcgtgttgc aggccccaat ggcgcagcgg
      961 ggtgtcgcgg tagccggcgc tgtgttgcag ccaggtggcc gtgtcgccgc gcaggtgggt
     1021 ccaaaagatg gtccagggtt ggcggtcgtc ggcggcgtag tcgtgggcca tgcccggcgg
     1081 cagccagagc aggtcgccgc tgccgacccg gtattcgcgc caggcctcgc cctcgcgcac
     1141 gcgcaacagg ccctggcctt cgctgcaata gatcagcagg tgctcgtcat ggcgctcgcg
     1201 gctcatgcgg tggtcgcggg cgcgccggta gtggcccagg gaggtggggt agaggtcgcg
     1261 gctgagcggg tggaccgcca gtttctccag cagcgccgcc gggatcggca agcgcaggct
     1321 gtcggccggc aggggccagt cggaggtgtg cgacatgtcg gtagccggct ccgcaatata
     1381 gtccatgaac aacacaattt agtcgatctc gcgaccttcc gcccagtgat agcttcttgg
     1441 ccaaagcgag gtccgaatcg acctcgtacc agtccgccac aacaataaaa gccggcctgg
     1501 ccggtgcagg agggttcatg tcagtcccag tccgccacct catcgcggga gccttcgtcg
     1561 aaggtctcgg cgcccagcgc atcccggtca gcaatcccct cgacaacagc accctggcgg
     1621 agatcgcctg cgccagcgcg gagcaggtcg agcaggcggt ggccagtgcc cgcgaaacct
     1681 tcgccagctg gaaggaaacc ccggtttccg agcgcgcgcg ggtgatgctg cgttaccagg
     1741 cgttgctcaa ggaacaccac gacgaactgg cgaagatcgt ctccagcgag ctgggcaaga
     1801 ctttcgagga cgccaagggc gacgtctggc gcggtatcga ggtggtcgaa cacgcctgca
     1861 acgtgccctc gctgctgatg ggcgagacgg tggagaacgt cgcccgcaac atcgacacct
     1921 acagcatcac ccagccgctg ggcgtgtgcg ttggcatcac cccgttcaac ttcccggcga
     1981 tgatcccgct gtggatgttc ccgctggcca tcgcctgcgg caacgccttc atcctcaagc
     2041 cttccgagca ggtgccgctg accagcgtgc ggctcgccga gctgttcctc gaggccggtg
     2101 cgccgaaggg cgtgctgcag gtggtgcatg gcggcaagga acaggtcgac cagttgctca
     2161 agcacccgca ggtgaaggcg gtgtccttcg tcggctcggt cgccgtcggc cagtacgtct
     2221 accacaccgg caccgcgcac aataagcgcg tgcagagctt cgccggggcg aagaaccaca
     2281 tggtgatcat gcccgacgcg gacaaggcac aggtcatcag caacctggtc ggtgcctcgg
     2341 tgggcgcggc cgggcagcgc tgcatggcga tctcggtggc ggtgctggtg ggggccgccc
     2401 gcgagtggat tccggaaatc cgcgacgcgc tggccaaggt ccgccccggc ccatgggacg
     2461 acagcggcgc cagctacggc ccggtgatca acccgcaggc caaggcgcgc atcgagcgcc
     2521 tgatcggcca gggcgtggag gagggcgcgc aactgctgct ggatggccgc ggctacaagg
     2581 tcgagggata cccggacggc aactgggtcg gcccgacgct gttcgccggg gtgcgcccgg
     2641 acatggcgat ctaccgcgaa gaggtgttcg gcccggtgct ctgcctggcc gaggtcgaca
     2701 gcctggagca ggcgatccgc ctgatcaacg aaagccccta cggcaacggc acctcgatct
     2761 tcaccagctc cggcgccgcg gcgcggacct tccagcacca catcgaggtc ggccaggtgg
     2821 gcatcaacat cccgattccg gtaccgctgc cgttcttctc cttcaccggt tggaagggct
     2881 cgttctacgg cgacctgcac gcctacggca agcagggcgt gcgcttctac accgagacca
     2941 agaccgtgac cgcgcgctgg ttcgacagcg acagcgtggc cgggaccaac ttctcgatcc
     3001 agatgcgctg aaagggagac ggaccgatga ccgacatcgc attcctcggc ctgggcaaca
     3061 tgggtgggcc gatggccgcc aacctgctca aggccggcca ccgggtgaat gtcttcgact
     3121 tgcagcccaa ggccgtgctg ggcctggtcg agcagggcgc gcagggcgcc gatagcgcct
     3181 tgcagtgctg cgaaggcgcc gaagtggtga tcagcatgct gccggccggg cagcacgtgg
     3241 aaagcctgta tctcggcgac gacggcctgc tcgcgcgggt cgccggcaag cccctgctga
     3301 tcgactgctc gaccatcgcc ccggagaccg cgcgcaaggt cgccgaggcc gccgcggcga
     3361 agggcctgac cctgctcgac gcgccggttt ccggcggcgt cggcggcgcc cgcgccggca
     3421 ccctgagctt catcgtcggc ggccccgccg aaggcttcgc gcgggcccgg ccggtcctcg
     3481 agaacatggg ccggaacatc ttccacgccg gcgatcacgg cgccggccag gtggcgaaga
     3541 tctgcaacaa catgctcctc ggcatcctca tggccggcac cgccgaggcc ctggcgctgg
     3601 gggtgaagaa cggcctcgac ccggcggtgc tgtccgaggt gatgaagcag agttccggcg
     3661 gcaactgggc gctgaacctc tacaacccct ggcccggggt gatgccgcag gcgccggcga
     3721 gcaacggcta tgccggcggt ttccaggtgc gcctgatgaa caaggacctc ggcctggcgc
     3781 tggccaacgc ccaggcggtg caggcctcga cgccgctcgg cgcgctggcg cgcaacctgt
     3841 tcagcctgca cgcccaggcc gatgccgagc acgaggggct ggacttctcc agcatccaga
     3901 agctctaccg cggcaaggac tgacccctac gaccaaaggt tgatggcggg tgcccggcac
     3961 ccgcccatac tccaaggctc actcactgct tcatccctaa taagaacaat cagagggctg
     4021 gaacatgacg tacctgcagc agcacgcccg ctcgatcgcc gagccggccg cgttctgggc
     4081 cgaacaggcc cgttccctgg cctggtacca ggcgcccgcg aacatcctcg aaagcctgcc
     4141 cgacggcacc catcgctggt tcgccgatgg ccgtctgaac agcgcctacc tggccctcga
     4201 ccggcagatc gaggaggggc gcggcgaaca gaccgcgctg atctacgact cgccggtgac
     4261 cggcacccag gaccgctaca gctacctgcg gctgcgcgac gaagtggcgc gcctggccgg
     4321 tgccctgcgg gccctcgggg tcggcaaggg cgaccgggtg atcatctaca tgccgatggt
     4381 gccgcaggcg gccatggcga tgctcgcctg cgcgcgcctg ggcgcggtgc attcggtggt
     4441 gttcggtggc ttcgctccct acgagctggc cctgcgcatc gacgacgcca cgccgaagct
     4501 ggtgctcacc gcttcctgcg ggctggagtt cgatcgggtg atcgaataca agccgctggt
     4561 ggacaaggcc ctcgaactgg cgatccacca gcctgcccac gtcatggtct ggcaacgccc
     4621 gcaggcgccc gcgcgcctgc acccgggccg cgacctggac tggcaggact gcctcgccgc
     4681 cgccgagccg gccgatccgc tgccggtggc cagcggcgat ccgctgtaca tcatgtacac
     4741 ctcgggcacc accggtaagc ccaagggtat cgtccgcgac aacggtggcc acgcggtcgc
     4801 ggtgcgctac gcggtccgca ctatctacgg catgcaggcc ggcgacgtct ggtgggggat
     4861 ctccgacgtc ggctgggtgg tcggccactc gctgatcgtc tacggcccgc tgatgtgcgg
     4921 ctgtaccacg gtgttctacg aaggcaagcc ggtgcgcacg ccggacgccg gcgcctactg
     4981 gcgggtgatc gaagagcaca gggtgaacag cctgttctgc gcgccgacgg cgatccgcgc
     5041 gatccgcaag gaagacccgc atggcgaacg ggtgaagcgc tacgacctgg gttcgctgcg
     5101 ccacctgttc ctggccggcg agaagctcga cagcagcacc cagcactggc tggaggagca
     5161 caccgggcgg ccggtccacg accattggtg gcagaccgag accggctggc cggtgaccgc
     5221 gccctgcatc gggatcgccg gccacgacct gcgggccggc tcgaccaacc gcgcggtgcc
     5281 cggctaccac gtgcaggtgc tggacgacga aggccggccg ctgggagcga accgccaggg
     5341 cgcgatcgtc atcgccctgc cgttgccgcc gggctgcgcg cagaccctct ggggcgacca
     5401 cccgcgctac ctgcagg
//