From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: PHO2=acid phosphatase [Yarrowia lipolytica, W29, Genomic, 2180 nt]
Date: 27 Jan 93 05:56:26 GMT
Distribution: bionet
Checksum: 22766 6

LOCUS       S118120      2180 bp    DNA             PLN       13-JAN-1993
DEFINITION  PHO2=acid phosphatase  [Yarrowia lipolytica, W29, Genomic, 2180 nt]
ACCESSION   S49387 X65225
KEYWORDS    .
SOURCE      Yarrowia lipolytica W29
  ORGANISM  Yarrowia lipolytica
            Unclassified.
REFERENCE   1  (bases 1 to 2180)
  AUTHORS   Treton,B.Y., Le Dall,M.T. and Gaillardin,C.M.
  TITLE     Complementation of Saccharomyces cerevisiae acid phosphatase
            mutation by a genomic sequence from the yeast Yarrowia lipolytica
            identifies a new phosphatase.
  JOURNAL   Curr. Genet. 22, 345-355 (1992)
  STANDARD  full automatic
COMMENT     This entry [NCBI gibbsq 118120] was created by the journal scanning
            component of NCBI/GenBank at the National Library of Medicine.
            This sequence comes from Fig. 4.
FEATURES             Location/Qualifiers
     CDS             914..1990
                     /note="APase;  orthophosphoric monoester phosphohydrolase;
                      For the protein sequence (NCBI gibbsq 118121):  Method:
                     conceptual translation supplied by author.  This sequence
                     comes from Fig. 4."
                     /gene="PHO2"
                     /product="acid phosphatase"
                     /codon_start=1
                     /translation="MKFSTIALPLLASAALAQTNSSHSGTNATSHNSTVPNENSKTTI
                     VVTNDDSWASANIRAFYDELKKEGYNVFMFAPALQQSGTGGTFVLPKNTTLAKGAEWG
                     SAPVGAPAWGQDEKDDHIWYFDGTPGAAVTFGFDYALPKFHNNITVDLVVSGPNEGWN
                     LGPFVYTLSGTEGAMYTSVLRGVPAIAFSGENKHTYYANASNSETASHNIYAKASTAI
                     VKNLLKNAKVRPSVLPYGVGLSVNLPLVGDIDPTGKCTDPKPIFTRQTGRGAITDKLV
                     FNETTGLFKYGDIKSDATKACLNGDCFLPDETDVINNWGCYSSISVVSTDYDAPGALA
                     AEAQFLNRGLVEFAPTGYGSFPGN"
     CDS             complement(1031..2002)
                     /note="Description: orf2 overlapping PHO2;  For the
                     protein sequence (NCBI gibbsq 118122):  Method: conceptual
                     translation supplied by author.  This sequence comes from
                     Fig. 4."
                     /codon_start=1
                     /translation="MISRLVTRERTVAGRSKLDQTSVEELSLGSKSTRGVIVGRDDRN
                     GGVAAPVVDDVSLIGKEAVSVQAGLGSIRLDISVLEQACSLVENQFVGDGASAGLSGE
                     DRLGISALSSRINVPNQRKIDRETDTVGQNRRSHLGVFEQVLDNGSGGLGVDVVGGGL
                     GVGGVGVVSVLVLSREGNCRNASQNRGVHGSLSSRQGVDEGTEVPALIGSRHNQVDGD
                     VVVELGQSVVETKGHGSSGGSVKVPDVVVLLVLAPSRSSNGSRAPLGTLGESSVLWEH
                     KGASGSRLLQGRGKHEHVVALLLELIVESSNVSRGPRIVIGDDNGGL"
BASE COUNT      524 a    646 c    482 g    528 t
ORIGIN      
        1 aaaaccatct ccatcacctt gtacgtcgta taacatctat aatccaccct caattactgc
       61 agccaagcca ctagcgacga cttaactttg cagagtccag ttatggcgta atatatccaa
      121 tgggacgagg agccctgcga cacgctgagc ccttgggttt cccccacctg cttgttttgt
      181 caaccagaaa ggtctcttgc ttgtcctctt gccatttgat ccccatttag cgctaaggtt
      241 aagccgacga tgacattgca aagtaagtct ctttctcacg ggaatcataa tatcgggtcc
      301 ccgcagatat gccagcatgt ctcagcggct gtgcaggtcg ttacacgtgt ctggtttggt
      361 tgatatgatt tactgaacgc ctctgctttg gtcctcttca cctctttgct cacagtaact
      421 tcacagacat acatcattag cccatgtcta attgatgaca agattgatct gataatctac
      481 cggcaccctc acaagtgggc aattagcaac caggcgatct gagcttgttt gggatggtgg
      541 tgactgcctt ccccaaaagc ctggcagaaa accaagccgt cgtagtgatt tttaggtcct
      601 actgaccctg ttatctcacc gtgtggagct gtcacgccca gtcactgcag gccgtttctc
      661 ttctcccact ttggcacggc actgtactaa tctatgcacc ttgtgcagca acgtgcagaa
      721 gcgtgcagaa acgtgcgcca acatgcacct acaccgacga gcgaaaaaac gaaccaaatg
      781 atagccaaac gatatccgaa atggtcaccc aggacgagac aaagacctgt ggatgtatat
      841 aaggaggagg gtcccgatcc aggattctca gaccagatcc tgtcatttgc tcactcgctc
      901 accccccctc ataatgaagt tctctaccat cgctcttcct cttctggcct cggctgctct
      961 ggcccagacc aactcttccc acagcggcac caacgccacc tcccacaact ctaccgtgcc
     1021 caatgagaac tcaaagacca ccattgtcgt caccaatgac gattcttggg cctctgctaa
     1081 cattcgagct ttctacgatg agctcaagaa ggagggctac aacgtgttca tgtttgcccc
     1141 tgccctgcaa cagtcgggaa ccggaggcac ctttgtgctc ccaaagaaca ctactctcgc
     1201 caagggtgcc gagtggggct cggctcccgt tggagctcct gcttggggcc aggacgagaa
     1261 ggacgaccac atctggtact ttgacggaac ccccggagct gccgtgacct ttggtttcga
     1321 ctacgctctg cccaagttcc acaacaacat caccgtcgac ctggttgtgt cgggacccaa
     1381 tgagggctgg aacctcggtc ccttcgtcta caccctgtct ggaactgagg gagccatgta
     1441 cacctctgtt ctgcgaggcg ttcctgcaat tgccttctct ggagagaaca agcacactta
     1501 ctacgccaac gcctccaact ccgagaccgc ctcccacaac atctacgcca aggcctccac
     1561 tgccattgtc aagaacctgc tcaaaaacgc caaggtgcga ccttctgttc tgccctacgg
     1621 tgtcggtctc tcggtcaatc ttcctctggt tggggacatt gatcctactg gaaagtgcac
     1681 tgatcccaag cctatcttca cccgacagac cggccgaggc gccatcaccg acaaactggt
     1741 tttcaacgag actacaggcc tgttcaagta cggagatatc aagtctgatg ctaccaaggc
     1801 ctgcctgaac ggagactgct tccttcccga tgagactgac gtcatcaaca actggggctg
     1861 ctactcctcc atttctgtcg tctctaccga ctatgacgcc cctggtgctc ttgctgccga
     1921 ggctcagttc ctcaacagag gtctggtcga gtttgctcct accggctacg gttcgttccc
     1981 tggtaactaa tcgagagatc atacacgcga gcgtatcgag cgcatcggtg cttgaacgct
     2041 ccaacaaaac attccttacg ataccattta gattctgtaa tattaatgat ttatgacaac
     2101 cataaaatga cggaaacgaa ttacttaaac cgaaagggtc tagtggatta aataatccaa
     2161 gattaaactg gaaactgttt
//