From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Rattus rattus steroid 5-alpha-reductase 2 mRNA, complete cds.
Date: 29 Dec 92 19:59:31 GMT
Distribution: bionet
Checksum: 14904 5

LOCUS       RATST25AR    1798 bp ss-mRNA            ROD       10-DEC-1992
DEFINITION  Rattus rattus steroid 5-alpha-reductase 2 mRNA, complete cds.
ACCESSION   M95058
KEYWORDS    steroid 5-alpha-reductase; steroid 5-alpha-reductase 2.
SOURCE      Rattus norvegicus (strain Sprague-Dawley) (library: Clonetech;
            Charon 4a) male adult testis cDNA to mRNA.
  ORGANISM  Rattus norvegicus
            Eukaryota; Animalia; Chorqata; Vertebrata; Mammalia; Theria;
            Eutheria; Rodentia; Myomorpha; Muridae; Murinae.
REFERENCE   1  (bases 1 to 1798)
  AUTHORS   Normington,K.D. and Russell,D.W.
  TITLE     Tissue distribution and kinetic characteristics of rat steroid 5
            alpha-reductaseisozymes. Evidence for distinct physiological
            functions
  JOURNAL   J. Biol. Chem. 267, 19548-19554 (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     mRNA            15..1789
                     /product="steroid 5-alpha-reductase 2"
     CDS             48..812
                     /function="catalyzes the reduction of delta 4,5 double
                     bonds in steroid"
                     /EC_number="1.3.99.5"
                     /standard_name="5a-reductase"
                     /evidence="EXPERIMENTAL"
                     /citation=[1]
                     /product="steroid 5-alpha-reductase 2"
                     /codon_start=1
                     /translation="MQIVCHQVPVLAGSATLATMGTLILCLGKPASYGKHTESVSSGV
                     PFLPARIAWFLQELPSFVVSVGMLAWQPRSLFGPPGNVLLALFSAHYFHRTFIYSLLT
                     RGRPFPAVLFLRATAFCIGNGLLQAYYLVYCAEYPEEWYTDVRFSFGVFLFILGMGIN
                     IHSDYTLRQLRKPGEVIYRIPRGGLFTYVSGANFLGEIIEWIGYALATWSVPAFAFAF
                     FTLCFLGMQAFYHHRFYLKMFKDYPKSRKALIPFIF"
BASE COUNT      487 a    436 c    412 g    463 t
ORIGIN      
        1 gaattccggc tgaggggcgg cagctaccaa ctgtgaccac aggcgagatg cagattgtct
       61 gccatcaggt cccggtgctg gcaggtagcg ccacattggc cactatgggg accctgatcc
      121 tgtgcttagg gaaacccgcc agttacggga aacacacaga gagtgtgtcg tcgggagttc
      181 ccttcctgcc ggcacgcatc gcctggttcc tgcaggagtt gccttccttt gtggtgtcgg
      241 tagggatgct cgcttggcag ccgcgctccc tcttcggacc gcccgggaat gtcctgctgg
      301 ctctcttctc tgcacattac ttccacagga catttattta ctcgttgctc acaagaggga
      361 ggcctttccc agcggtgctg tttttgagag ccactgcctt ctgcataggg aacggactcc
      421 ttcaagccta ctacctggtt tactgcgcag aataccccga ggagtggtac acagatgtgc
      481 ggtttagctt tggtgtcttc ctgtttattc tggggatggg aatcaacatc cacagtgact
      541 acaccctgcg ccagctcagg aagcctggag aagtcatcta taggattcct cgaggtggct
      601 tgtttacgta tgtctctgga gccaatttcc tgggcgagat tattgaatgg attggctacg
      661 ccttggccac gtggtccgtc ccagccttcg ctttcgcctt tttcacactt tgtttcctgg
      721 ggatgcaagc cttttaccac cacaggttct accttaagat gtttaaggat taccccaaat
      781 ctaggaaagc tctcattcca ttcatctttt aaagaacccc aattttaaag agcaaagttt
      841 ctgtggagga actgctcagc tgctgaaact ataaactgta aactataaca gtgtcttgct
      901 cacatgtata tatggtgatg tatgtgttaa aaggtctctt gttattccag ttgcttgagg
      961 catgcagggt catgcctgct tagcctatac tcctttctgc ccagggagct ctaacccaat
     1021 ttccttttgg agcttcacag aggccatttt attcttagct ataccatcta gaagcttctt
     1081 gtttcccacg gtatgatctg aagaggtaat tgttttctta ttccatgtca ctttggggaa
     1141 gttctgcagt caaaaccctt tggactgaca taatgaataa tgaaattata gcaattgcag
     1201 gaaagtgggt agaactggaa attattatat taagcaacct aatcctgact cagaaagaca
     1261 aatactgcat gtttgtttca tgtgcaggtt ctagatttta atatatatct aactgttcat
     1321 atgtgtgtgt tgggggtcat atagttcatt gatttggaaa ggagatcatg agaagagaaa
     1381 atgggtcttg ctgggaggaa aaaaagaggg aaataaaatt tgtgtggcag agagaggcta
     1441 ctgcaggagg aaggggagca tcaggagaaa tgtaggagag aaggggagga ggattggcaa
     1501 aaataaagca tgtatgaaaa tgtccataat gaaaccaatt actttatatg ttggttttaa
     1561 aaaacaacca gccagccagt caataaacca tccatccagg cagtcaatca accagctggc
     1621 cagccagcca gccggccagc caacctacca accaaccaac tatccaacca accaaccaac
     1681 caaccagcca accaactaac caaccaacca acaaatcaac caaccaacca aacagccagc
     1741 caccaaccaa ccaaccaaca aatcaaccaa gcactcaaac acgacaaccc cggaattc
//