From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: B.thuringiensis cryV gene for delta-endotoxin
Date: 24 Jul 92 10:05:09 GMT
Distribution: bionet
Checksum: 32980     7

LOCUS       BACCRYV      2965 bp ds-DNA             BCT       24-JUL-1992
DEFINITION  B.thuringiensis cryV gene for delta-endotoxin
ACCESSION   X62821
KEYWORDS    cry gene; cryV gene; delta-endotoxin; insecticidal delta-endotoxin;
            insecticidal protein.
SOURCE      Bacillus thuringiensis DNA.
  ORGANISM  Bacillus thuringiensis
            Prokaryotae; Firmicutes; Endospore-forming rods and cocci;
            Bacillaceae.
REFERENCE   1  (bases 1 to 2965)
  AUTHORS   Ely,S.
  JOURNAL   Unpublished (1991)
  STANDARD  full automatic
REFERENCE   2  (sites)
  AUTHORS   Tailor,R., Tippett,J., Gibb,G., Pells,S., Pike,D., Jordan,L. and
            Ely,S.
  TITLE     Identification and characterisation of a novel Bacillus
            thuringiensis delta-endotoxin entomocidal to coleopteran and
            lepidopteran larvae
  JOURNAL   Mol. Microbiol. 6, 1211-1217 (1992)
  STANDARD  full staff_review
COMMENT     *source: strain=ssp. Kurstaki, JHCC4835; *source: sub_strain=
            JHCC4835;
            For related sequence see Chambers J.A. et al, J.Bacteriol. 173:
            3966-3976(1991)
            From EMBL    entry BTCRYV;  dated 19-MAY-1992.
FEATURES             Location/Qualifiers
     RBS             343..347
     CDS             355..2514
                     /product="delta-endotoxin"
                     /gene="cryV"
                     /codon_start=1
                     /translation="MKLKNQDKHQSFSSNAKVDKISTDSLKNETDIELQNINHEDCLK
                     MSEYENVEPFVSASTIQTGIGIAGKILGTLGVPFAGQVASLYSFILGELWPKGKNQWE
                     IFMEHVEEIINQKISTYARNKALTDLKGLGDALAVYHDSLESWVGNRNNTRARSVVKS
                     QYIALELMFVQKLPSFAVSGEEVPLLPIYAQAANLHLLLLRDASIFGKEWGLSSSEIS
                     TFYNRQVERAGDYSYHCVKWYSTGLNNLRGTNAESWVRYNQFRRDMTLMVLDLVALFP
                     SYDTQMYPIKTTAQLTREVYTDAIGTVHPHPSFTSTTWYNNNAPSFSAIEAAVVRNPH
                     LLDFLEQVTIYSLLSRWSNTQYMNMWGGHKLEFRTIGGTLNISTQGSTNTSINPVTLP
                     FTSRDVYRTESLAGLNLFLTQPVNGVPRVDFHWKFVTHPIASDNFYYPGYAGIGTQLQ
                     DSENELPPEATGQPNYESYSHRLSHIGLISASHVKALVYSWTHRSADRTNTIEPNSIT
                     QIPLVKAFNLSSGAAVVRGPGFTGGDILRRTNTGTFGDIRVNINPPFAQRYRVRIRYA
                     STTDLQFHTSINGKAINQGNFSATMNRGEDLDYKTFRTVGFTTPFSFLDVQSTFTIGA
                     WNFSSGNEVYIDRIEFVPVEVTYEAEYDFEKAQEKVTALFTSTNPRGLKTDVKDYHID
                     QVSNLVESLSDEFYLDEKRELFEIVKYAKQLHIERNM"
BASE COUNT     1023 a    450 c    543 g    949 t
ORIGIN
        1 catatgtata gagcaactta atcaagcaga gatattttca cctatcgatg aaaatatctc
       61 tgctttttct ttttttattt ggtatatgct ttacttgtaa tcgaaaataa agcactaata
      121 agagtattta taggtgtttg aagttatttc agttcatttt taaagaaggt ttaaagacgt
      181 tagaaagtta ttaaggaata atatttatta gtaaattcca catatattat ataattaatt
      241 atgaaatata tgtataaatt gaaaatgctt tatttgacat tacagctaag tataattttg
      301 tatgaataaa attatatctg aaaattaaat aatagtataa gtggagggat taatatgaaa
      361 ctaaagaatc aagataagca tcaaagtttt tctagcaatg cgaaagtaga taaaatctct
      421 acggattcac taaaaaatga aacagatata gaattacaaa acattaatca tgaagattgt
      481 ttgaaaatgt ctgagtatga aaatgtagag ccgtttgtta gtgcatcaac aattcaaaca
      541 ggtattggta ttgcgggtaa aatacttggt accctaggcg ttccttttgc aggacaagta
      601 gctagtcttt atagttttat cttaggtgag ctatggccta aggggaaaaa tcaatgggaa
      661 atctttatgg aacatgtaga agagattatt aatcaaaaaa tatcaactta tgcaagaaat
      721 aaagcactta cagacttgaa aggattagga gatgccttag ctgtctacca tgattcgctt
      781 gaaagttggg ttggaaatcg taataacaca agggctagga gtgttgtcaa gagccaatat
      841 atcgcattag aattgatgtt cgttcagaaa ctaccttctt ttgcagtgtc tggagaggag
      901 gtaccattat taccgatata tgcccaagct gcaaatttac atttgttgct attaagagat
      961 gcatctattt ttggaaaaga gtggggatta tcatcttcag aaatttcaac attttataac
     1021 cgtcaagtcg aacgagcagg agattattcc taccattgtg tgaaatggta tagcacaggt
     1081 ctaaataact tgaggggtac aaatgccgaa agttgggtac gatataatca attccgtaga
     1141 gacatgactt taatggtact agatttagtg gcactatttc caagctatga tacacaaatg
     1201 tatccaatta aaactacagc ccaacttaca agagaagtat atacagacgc aattgggaca
     1261 gtacatccgc atccaagttt tacaagtacg acttggtata ataataatgc accttcgttc
     1321 tctgccatag aggctgctgt tgttcgaaac ccgcatctac tcgattttct agaacaagtt
     1381 acaatttaca gcttattaag tcgatggagt aacactcagt atatgaatat gtggggagga
     1441 cataaactag aattccgaac aataggagga acgttaaata tctcaacaca aggatctact
     1501 aatacttcta ttaatcctgt aacattaccg ttcacttctc gagacgtcta taggactgaa
     1561 tcattggcag ggctgaatct atttttaact caacctgtta atggagtacc tagggttgat
     1621 tttcattgga aattcgtcac acatccgatc gcatctgata atttctatta tccagggtat
     1681 gctggaattg ggacgcaatt acaggattca gaaaatgaat taccacctga agcaacagga
     1741 cagccaaatt atgaatctta tagtcataga ttatctcata taggactcat ttcagcatca
     1801 catgtgaaag cattggtata ttcttggacg catcgtagtg cagatcgtac aaatacaatt
     1861 gagccaaata gcattacaca aataccatta gtaaaagctt tcaatctgtc ttcaggtgcc
     1921 gctgtagtga gaggaccagg atttacaggt ggggatatcc ttcgaagaac gaatactggt
     1981 acatttgggg atatacgagt aaatattaat ccaccatttg cacaaagata tcgcgtgagg
     2041 attcgctatg cttctaccac agatttacaa ttccatacgt caattaacgg taaagctatt
     2101 aatcaaggta atttttcagc aactatgaat agaggagagg acttagacta taaaaccttt
     2161 agaactgtag gctttaccac tccatttagc tttttagatg tacaaagtac attcacaata
     2221 ggtgcttgga acttctcttc aggtaacgaa gtttatatag atagaattga atttgttccg
     2281 gtagaagtaa catatgaggc agaatatgat tttgaaaaag cgcaagagaa ggttactgca
     2341 ctgtttacat ctacgaatcc aagaggatta aaaacagatg taaaggatta tcatattgac
     2401 caggtatcaa atttagtaga gtctctatca gatgaattct atcttgatga aaagagagaa
     2461 ttattcgaga tagttaaata cgcgaagcaa ctccatattg agcgtaacat gtagaattaa
     2521 aatctaccta aatccagaaa aataaaaggg ttaaatatac aattcttgta ccaatatttt
     2581 gagtgattag atgtaggatg aaatttaatt gtatgctatt taacagtaga gatattaaaa
     2641 attaatttat ctatacatta atagtataga catacaaaca taagagagca ttgtcttttc
     2701 gtaggctaca atgctctcta tttactattt atttttcttt tgtatcttca aattgacgtt
     2761 gttctaagcg ttctattgca gctcgtcgtt tagtatcatc aatgtttgta taaagagatg
     2821 ttgtttccat agaattatgt cccatttgat ttgctaataa tactaaatct ttattttcat
     2881 tatagtgatt agtagcataa gtatgacgta atttatgagg gcttttcttt tcatcaaaag
     2941 cccttgtgta tttctctgta agctt
//