From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: cmr=chloramphenicol-resistance gene [Rhodococcus fascians, NCPPB
Date: 27 Jan 93 05:51:56 GMT
Distribution: bionet
Checksum: 48097 6

LOCUS       S114628      2716 bp    DNA             BCT       26-JAN-1993
DEFINITION  cmr=chloramphenicol-resistance gene [Rhodococcus fascians, NCPPB
            1675, pRF2, Plasmid, 2716 nt]
ACCESSION   S45617 Z12001
KEYWORDS    .
SOURCE      Rhodococcus fascians pRF2 NCPPB 1675
  ORGANISM  Rhodococcus fascians
            Unclassified.
REFERENCE   1  (bases 1 to 2716)
  AUTHORS   Desomer,J., Vereecke,D., Crespi,M. and Van Montagu,M.
  TITLE     The plasmid-encoded chloramphenicol-resistance protein of
            Rhodococcus fascians is homologous to the transmembrane
            tetracycline efflux proteins.
  JOURNAL   Mol. Microbiol. 6, 2377-2385 (1992)
  STANDARD  full automatic
COMMENT     This entry [NCBI gibbsq 114628] was created by the journal scanning
            component of NCBI/GenBank at the National Library of Medicine.
            This sequence comes from Fig. 2.  The authors begin their numbering
            at -713.  Zero is not present in the numbering.
FEATURES             Location/Qualifiers
     CDS             993..2168
                     /note="GTG start codon.  Description:
                     chloramphenicol-resistance gene;  tetracycline efflux
                     protein homolog;  For the protein sequence (NCBI gibbsq
                     114629):  Method: conceptual translation supplied by
                     author.  This sequence comes from Fig. 2.
                     Author-given protein sequence differs from the conceptual
                     translation for reasons explained in citation"
                     /gene="cmr"
                     /product="CMR"
                     /codon_start=1
                     /translation="MPFAIYVLGIAVFAQGTSEFMLSGLIPDMAQDLQVSVPTAGLLT
                     SAFAIGMIIGAPLMAIVSMRWQRRRALLTFLITFMVVHVIGALTDSFGVLLVTRIVGA
                     LANAGFLAVALGAAMSMVPADMKGRATSVLLGGVTIACVVGVPGGALLGELWGWRASF
                     WEVVLISAPAVAAIMASTPADSPTDSVPNATRELSSLRQRKLQLILVLGALINGATFC
                     SFTYLAPTLTDVAGFDSRWIPLLLGLFGLGSFIGVSVGGRLADTRPFQLLVAGSAALL
                     VGWIVFAITASHPVVTLVMLFVQGTLSFAVGSTLISRVLYVADGAPTLGGSFATAAFN
                     VGAALGPALGGVAIGIGMGYRAPLWTSAALVALAIVIGAATWTRWREPRPALDTVPP"
BASE COUNT      458 a    813 c    817 g    628 t
ORIGIN      
        1 tctagagcgg acgcagggcg cgccggcctc gttcgcattt gctgctctcg cccgcccgca
       61 gcgcggttgc atgctgcaat ggtggaccga actggattcg tgcaccgacg tctctgtgtg
      121 atgcccgacg ctgcgcgatc cgtctacgtg ctcgacttcg gcccagatgg cgttctgtat
      181 gagatcagat gaccggtgcg ttgggttggc ggcggatgaa gtccgccatt tcggggacag
      241 tgaaggcaac caggccgcgg gagaccagat gagtcctttg cgtatgcagg agtcacggtg
      301 cggtgccatc gattgcgggt tcgatccgag ggactcggcg agggttgctg tccgcggagt
      361 gtctgttccg ctgcgtgcga tggcgaccag atagcgcttt tcatgtcggt gcggtcgagt
      421 ccctgcaata gctctgtagg agctttacga cacggaactg tgccttcgct gggtgctctc
      481 tgcacatcgt tagcgcctac cgcgtgaaag tgtgccccat cgagcgactc acagtccact
      541 gcgtactccg gtagagcaat gagtgctatc cctggaatct gttgttgtgc agcgcttgtg
      601 ggcattgttg tgcggtgtac ggaccaactg cgcgaacgtc gcgtcatgcc gtctgggata
      661 cgcgacgccc cgaatcggtt ggacaatcac atccgacatt gctaacttcg atgacatgtc
      721 cagccccaag gcttccagac tctagccacc ggcagcagcc ggtggccacg caccttgcgt
      781 tcgatgccgt tctctctacg gctcaatttg cagtgcgcga ttcgtggcac cctctgccct
      841 ccgaagctta agtctggcct tggcggctca cattctttca ctgtggcgct gtcaggtcac
      901 ccgctccgag atcgatttgc atcgctcctg aatccgacga tgcaaacaat cccgtagcct
      961 cccttgacct ttctcagaca cggagtctca cagtgccatt cgccatctat gtcctgggta
     1021 ttgctgtatt cgcccagggc acatcggaat tcatgctgtc cggactcata ccggatatgg
     1081 ctcaggatct acaggtttcg gtccccactg caggacttct cacttcggca ttcgcaatcg
     1141 gcatgatcat cggtgccccg ttgatggcaa ttgtcagtat gcggtggcaa cgtcgacgag
     1201 cgctcttgac cttcctcatc acttttatgg ttgtgcatgt catcggcgca ctcaccgaca
     1261 gtttcggcgt cttgctggtc acccggatcg taggagcact ggccaacgcc ggtttcctgg
     1321 ctgtagcgct gggcgcagcc atgtcgatgg ttcctgccga catgaaggga cgagcgacct
     1381 cagttctact gggcggagtg accatcgcct gcgtagttgg agtcccgggc ggagcgctat
     1441 tgggcgaact gtggggatgg cgcgcctcgt tctgggaggt agtgctgatt tccgcaccgg
     1501 cagtggcagc gatcatggca tcgacccctg ctgattcccc tacagattct gttccgaacg
     1561 cgacccgcga actgtcctcg ctgcgtcaac gcaaacttca actgatcttg gtgctgggcg
     1621 cgctgatcaa cggtgccacc ttctgttcct tcacctacct ggctccgacg ctcaccgacg
     1681 tcgccgggtt cgactctcgc tggatccctt tgcttctcgg actgttcgga ctggggtcgt
     1741 tcatcggcgt cagtgtcggt ggccggctcg ctgacacccg tccgtttcaa ttgctggtgg
     1801 cgggctcggc agctcttctg gtcgggtgga tcgtgttcgc tatcactgcc tctcacccgg
     1861 tagtgaccct ggtgatgctg ttcgtgcaag gaacgctgtc gttcgctgtg gggtcgacgt
     1921 tgatctcgcg agtgctctac gtcgccgacg gtgctccgac tttgggggga tccttcgcta
     1981 cggctgcctt caatgtcgga gccgcattgg ggccggccct cggcggtgtg gccatcggta
     2041 tcggaatggg ctatcgcgct ccactgtgga ccagcgcggc tctggtggca cttgcgatcg
     2101 tgatcggtgc cgcgacgtgg acgcgttggc gggaaccacg tccagcgctg gacaccgttc
     2161 ctccgtgacg gcatcgacgg gtcgtgcgtg cgattgcgcc atttgtcgtc cttggtgctt
     2221 tccgcacgcc cgatccgccg tcaccgcgaa gccacgtgta agcggtcctc gccgctcgca
     2281 ctaagtagga aatttgctac gcatccccag gcagatttat cgagagctac gctcccacag
     2341 aacctttgga gaaattgaac atgactgcat tcgatgaatc gaacggccct tcgccgatcc
     2401 ttgcgcccgc gctcgtcgac gacgcacagg gcacatcctt acccgggcgg cgtctggccc
     2461 cttcctcagc agaagcgaca ataatgcaat tgagcaggcg gcaggaacaa ataattgagc
     2521 tcgtcgctga aggctactca ggcaaggaag tagcgcgtgc actcggaatg tcaccgaaaa
     2581 ccgttgagac gcatatccaa cgaattttcg accggtacgg cgttcggaat cgtgcgggtg
     2641 ttgtagcgag gtggatggct agtaagcacc tcgcggcgag ctgatagagc acagctgtgt
     2701 ccggccatac ggatcc
//