From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human Line-1 repeat mRNA with 2 open reading frames.
Date: 29 Jul 92 10:03:06 GMT
Distribution: bionet
Checksum: 54036    10

LOCUS       HUMRSLINE    5976 bp ss-mRNA            PRI       29-JUL-1992
DEFINITION  Human Line-1 repeat mRNA with 2 open reading frames.
ACCESSION   M19503 M59450
KEYWORDS    LINE repetitive sequence.
SOURCE      Human teratocarcinoma cell line NTera2D1, cDNA to mRNA, clone cD11.
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 5976)
  AUTHORS   Skowronski,J., Fanning,T.G. and Singer,M.F.
  TITLE     Unit length Line-1 transcripts in human teratocarcinoma cells
  JOURNAL   Mol. Cell. Biol. 8, 1385-1397 (1988)
  STANDARD  simple staff_entry
REFERENCE   2  (sites)
  AUTHORS   Swergold,G.D.
  TITLE     Identification, characterization, and cell specificity of a human
            line-1 promoter
  JOURNAL   Mol. Cell. Biol. 10, 6718-6729 (1990)
  STANDARD  full staff_review
COMMENT     Draft entry and computer-readable sequence for [1] kindly provided
            by M.Singer, 06-APR-1988.
            There is an in-frame stop codon in ORF2 at positions 2818-2820.
FEATURES             Location/Qualifiers
     repeat_region   <1..5976
                     /note="Line 1 repeat"
     CDS             877..1893
                     /note="ORF1"
                     /note="putative"
                     /codon_start=1
                     /translation="MGKKQNRKTGNSKTQSTSPPPKERSSSPATDQSWMENDFDELRE
                     EGFRRSNYSELREDIQTKGKEVENFEKNLEECITRITNTEKCLKELMELKTKARELRE
                     ECRSLRSQCDQLEERVSAMEDEMNEMKQEGKFREKRIKRNEQSLQEIWDYVKRPNLRL
                     IGVPESDAENGTKLENTLQDIIQETFPNLARQANVQIQEIQRTPQRYSSRRATPRHII
                     VRFTKVEMKEKMLRAAREKGRVTLKGKPIRLTADLSAETLQARREWGPILNILKEKNF
                     QPRISYPAKLSFISEGEIKYFTDKQMLTDFVTSRPALKELLKEALNMERNNRYQPLQN
                     HAKM"
     misc_feature    1930..5784
                     /note="ORF2"
                     /note="putative"
BASE COUNT     2289 a   1328 c   1213 g   1142 t      4 others
ORIGIN      631 bp upstream of BglII site.
        1 cctccggtct acagctccca gcgtgagcga cgcagaagac gggtgatttc tgcatttcca
       61 tctgaggtac cgggttcatc tcactaggga gtgccagaca gtgggcgcag gccagtgggt
      121 gcgcgcaccg tgcggagccg aagcagggcg aggcattgcc tcacctggga agcgcaaggg
      181 gtcagggagt tccctttccg agtcaaagaa aggggtgacg gacgcacctg gaaaatcggg
      241 tcactcccac ccgaatattg cgcttttcag accggcttaa aaaacggcgc accacgagac
      301 tatatcccac acctggcctt cggagggtcc tacgcccacg gaatctcgct gattgctagc
      361 acagcggtct gagatcaaac tgcaaggccg cagcaaggct gggggagggg cgcccgccat
      421 tgcccaggct tgcttaggta aacaaagcag ccggggaagc tcgaactggg tgcagcccac
      481 cacagctcaa ggaggcctgc ctgcctctgt aggctccacc tctgggggca gggcacagac
      541 aaacaaaaag acagcagtaa cctctgcaga cttaaatgtc cctgtctgac agctttgaag
      601 agagcagtgg ttctcccagc acgcagctgg agatctgaga acgggcagac tgcctcctca
      661 agtgggtccc tgacccctga cccccgagca gcctaactgg gaggcacccc ccagcagggg
      721 cacactgaca cctcacacgg cagggtattc caacagacct gcagctgagg gtcctgtctg
      781 ttagaaggaa aactaacaaa cagaaaggac atccacaccg aaaacgcatc tgtacatcac
      841 catcatcaaa gaccaaaagt agataaaacc acaaagatgg ggaaaaaaca gaacagaaaa
      901 actggaaact ctaaaacgca gagcacctct cctcctccaa aggaacgcag ttcctcacca
      961 gcaacggacc aaagctggat ggagaatgat tttgacgagc tgagagaaga aggcttcaga
     1021 cgctcaaatt actctgagct acgggaggac attcaaacca aaggcaaaga agttgaaaac
     1081 tttgaaaaaa atttagaaga atgtataact agaataacca atacagagaa gtgcttaaag
     1141 gagctgatgg agctgaaaac caaggctcga gaactacgtg aagaatgcag aagcctcagg
     1201 agccaatgcg atcaactgga agaaagggta tcagcaatgg aagatgaaat gaatgaaatg
     1261 aagcaagaag ggaagtttag agaaaaaaga ataaaaagaa acgagcaaag cctccaagaa
     1321 atatgggact atgtgaaaag accaaatcta cgtctgattg gtgtacctga aagtgatgcg
     1381 gagaatggaa ccaagttgga aaacactctg caggatatta tccaggagac tttccccaat
     1441 ctagcaaggc aggccaacgt tcagattcag gaaatacaga gaacgccaca aagatactcc
     1501 tcgagaagag caactccaag acacataatt gtcagattca ccaaagttga aatgaaggaa
     1561 aaaatgttaa gggcagccag agagaaaggt cgagttaccc tcaaagggaa gcccatcaga
     1621 ctaacagcgg atctctcggc agaaacccta caagccagaa gagagtgggg gccaatattg
     1681 aacattctta aagaaaagaa ttttcaaccc agaatttcat atccagccaa actaagcttc
     1741 ataagtgaag gagaaataaa atactttaca gacaagcaaa tgctgaccga ttttgtcacc
     1801 agcaggcctg ccctaaaaga gctcctgaag gaagcgctaa acatggaaag gaacaaccgg
     1861 taccagccac tgcaaaatca tgccaaaatg taaagaccat cgagactagg aagaaactgc
     1921 atcaactaac gagcaaaatc accagctaac atcataatga caggatcaaa ttcacacata
     1981 acaatattaa ctttaaatgt aaatggacta aattctccaa ttaaaagaca cagactggca
     2041 agttggataa agagtcaaga cccatcagtg tgctgtattc aggaaaccca tctcatgtgc
     2101 agagacacac ataggctcaa aataaaagga tggaggaaga tctaccaagc aaatggaaaa
     2161 caaaaaaagg caggggttgc aatcctagtc tctgataaaa cagactttaa accaacaaag
     2221 atcaaaagag acaaagaagg ccattacatg atggtaaagg gatcaattca acaagaggag
     2281 ctaactatcc taaatatgta tgcacccaat acaggagcac ccagattcat aaagcaagtc
     2341 ctgagtgacc tacaaagaga cttagactcc cacacattaa taatgggaga ctttaacacc
     2401 ccactgtcaa cattagacag atcaacgaga cagaaagtca acaaggatac ccaggaattg
     2461 aactcagctc tgcaccaagc agacctaata gacatctaca gaactctcca ccccaaatca
     2521 acagaatata catttttttc agcaccacac cacagctatt ccaaaattga ccacatactt
     2581 ggaagtgaag ctctcctcag caaatgtaaa agaacagaaa ttataacaaa ctatctctca
     2641 gaccacagtg caatcaaact agaactcagg attaagaatc tcactcaaag ccgctcaact
     2701 acatggaaac tgaacaacct gctcctgaat gactactggg tacataacga aatgaaggca
     2761 gaaataaaga tgttctttga aaccaacgag aacaaagaca caacatacca gaatctctag
     2821 gacgcattca aagcagtgtg tagagggaaa tttatagcac taaatgccca caagagaaag
     2881 caggaaagat ccaaaattga caccctaaca tcacaattaa aagaactaga aaagcaagag
     2941 caaacacatt caaaagctag cagaaggcaa gaaataacta aaatcagagc agaactgaag
     3001 gaaatagaga cacaaaaaac ccttcaaaaa atcaatgaat ccaggagctg gttttttgaa
     3061 aggatcaaca aaattgatag accgctagca agactaataa agaaaaaaag agagaagaat
     3121 caaatagaca caataaaaaa tgataaaggg gatatcacca ccgatcccac agaaatacaa
     3181 actaccatca gagaatacta caaacacctc tacgcaaata aactagaaaa tctagaagaa
     3241 atggatacat tccttgacac atacactctc ccaagactaa accaggaaga agttgaatct
     3301 ctgaatagac caataacagg agctgaaatt gtggcaataa tcaatagttt atcaaccaaa
     3361 aagagtccag gaccagatgg attcacagcc gaattctacc agaggtacaa ggaggaactg
     3421 gtaccattct ttctgaaact attccaatca atagaaaaag agggaatcct ccctaactca
     3481 ttttatgagg ccagcatcat tctgatacca aagccgggca gagacacaac caaaaaagag
     3541 aattttagac caatatcctt gatgaacatt gatgcaaaaa tcctcaataa aatactggca
     3601 aaccaaatcc agcagcacat caaaaagctt atccaccatg atcaagtggg cttcatccct
     3661 gggatgcaag gctggttcaa tatacgcaaa tcaataaatg taatccagca tataaacaga
     3721 gccaaagaca aaaaccacat gattgtctca atagatgcag aaaaagcctt tgacaaaatt
     3781 caacaaccct tcatgctaaa aactatcaat aaaataggta ttgatgggat gtatttcaaa
     3841 ataataagag ctatctatga caaaaccaca gccaatatca tactgaatgg gcaaaaactg
     3901 gaagcattcc ctttgaaaac cggcacaaga cagggatgcc ctctctcacc gctcctattc
     3961 aacatagtgt tggaatttct ggccagggca atcaggcagg agaaggaaat aaagggtatt
     4021 caattaggaa aagaggaagt caaattgtcc ctgtttgcag atgacatgat tgttgatcta
     4081 gaaaacccca tcgtctcagc ccaaaatctc cttaagctga taagcaactt cagcaaantc
     4141 tcaggataca aaatcaatgt acaaaaatca caagcattcc tatacaccaa caacagacaa
     4201 acagagagcc aaatcatgag tgaactccca ttcacaattg cttcaaagag aataaaatac
     4261 ctaggaatcc aacttacaag ggatgtgaag gacctcttca aggagaacta caaaccactg
     4321 ctcaaggaaa taaaagagga tacaaaaaaa tggaagaaca ntccatgctc atgggtagga
     4381 agaatcaata tcgtgaaaat ggccatactg cccaaggtaa tttacagatt caatgccatc
     4441 cccatcaagc taccaatgac tttcttcaca gaattggaaa aaactacttt aaagttcata
     4501 tggaaccaaa aaagagcccg cattgccaag tcaatcctaa gccaaaagaa caaagctgga
     4561 ggcatcacac tacctgactt caaactatac tacaaggcta cagtaaccaa aacagcatgg
     4621 tactggtacc aaaacagaga tatagatcaa tggaacagaa cagagccctc agaaataacg
     4681 nngcttacct acaactatct gatctttgac aaacctgaga aaaacaagca atggggaaag
     4741 gattccctat ttaataaatg gtgctgggaa aactggctag ccatatgtag aaagctgaaa
     4801 gtggatccct tccttacacc ttatacaaaa atcaattcaa gatggattaa agacttaaac
     4861 gttagaccta aaaccataaa aaccctagaa gaaaacctag gcattaccat tcaggacata
     4921 ggcgtgggca aggacttcat gtccaaaaca ccaaaagcaa tggcaacaaa agccaaaatt
     4981 gacaaatggg atctaattaa actaaagagc ttctgcacag caaaagaaac taccatcaga
     5041 gtgaacaggc aacctacaac atgggagaca attttcacaa cctactcatc tgacaaaggg
     5101 ctaatatcca gaatctacaa tgaactcaaa caaatttaca agaaaaaaac aaacaacccc
     5161 atcaaaaagt gggcgaagga catgaacaga cacttctcaa aagaagacat ttatgcagcc
     5221 aaaaaacaca tgaaaaaatg ctcatcatca ctggccatca gagaaatgca aatcaaaacc
     5281 actatgagat atcatctcac accagttaga atggcaatca ttaaaaagtc aggaaacaac
     5341 aggtgctgga gaggatgtgg agaaatagga acacttttac actgttggtg ggactgtaaa
     5401 ctagttcaac cattgtggaa gtcagtgtgg cgattcctta gggatctaga actagaaata
     5461 ccatttgacc cagccatccc attactgggt atatacccaa atgactataa atcatgctgc
     5521 tataaagaca catgcacacg tatgtttatt gcggcattat tcacaatagc aaagacttgg
     5581 aaccaaccca aatgtccaac aatgatagac tggattaaga aaatgtggca catatacacc
     5641 atggaatact atgcagccat aaaaaatgat gagttcatgt cctttgtagg gacatggatg
     5701 aaattggaaa ccatcattct cagtaaacta tcgcaagaac aaaaaaccaa acaccgcata
     5761 ttctcactca taggtgggaa ttgaacaatg agatcacatg gacacaggaa ggggaatatc
     5821 acactctggg gactatggtg gggagggggg aggggggagg gatagcattg ggagatatac
     5881 ctaatgctag atgacgagtt agtggtgcag tgcaccagca tggcacatgt atacatatgt
     5941 aactaacctg cacaatgtgc acatgtaccc taaaac
//