From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Bacillus firmus Cd2+ efflux system accessory protein (cadC) and
Date: 8 Apr 92 10:00:24 GMT
Article-I.D.: genbank.Apr.8.03.00.23.1992.16052
Distribution: bionet
Checksum: 36763     7

LOCUS       BACTNPRAB    2749 bp ds-DNA             BCT       08-APR-1992
DEFINITION  Bacillus firmus Cd2+ efflux system accessory protein (cadC) and
            cadmium efflux ATPase (cadA) gene, complete cds.
ACCESSION   M90750
KEYWORDS    cadmium-efflux ATPase; cadmium-efflux system accessory protein.
SOURCE      Bacillus firmus (strain OF4) DNA.
  ORGANISM  Bacillus firmus
            Prokaryota; Bacteria; Firmicutes; Endospore-forming rods and cocci;
            Bacillaceae.
REFERENCE   1  (bases 1 to 2749)
  AUTHORS   Ivey,D.M., Guffanti,A.A., Shen,Z., Kudyan,N. and Krulwich,T.A.
  TITLE     The cadC gene product of alkaliphilic Bacillus firmus OF4 partially
            restores Na+-resistance to an Escherichia coli strain lacking a
            Na+/H+ antiporter (NhaA)
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     RBS             116..119
                     /gene="cadC"
     CDS             127..495
                     /function="putative Cd2+ sequestration and delivery"
                     /product="cadmium-efflux system accessory protein"
                     /gene="cadC"
                     /codon_start=1
                     /translation="MNKKDTCEIFCYDEEKVNRIQGDLKTIDIVSVAQMLKAIADENR
                     AKITYALCQDEESCVCDIANIIGITAANASHHLRTLHKQGIVRYRKEGKLAFYSLDDE
                     HIRQIMMIVLEHKKEVNVNV"
                     /citation=[1]
     RBS             470..478
                     /gene="cadA"
     CDS             488..2659
                     /function="cadmium resistance"
                     /product="cadmium-efflux ATPase"
                     /gene="cadA"
                     /codon_start=1
                     /translation="MSDQKAITSEQEMKAYRVQGFTCANCAGKFEKNVKQLSGVEDAK
                     VNFGASKIAVYGNATIEELEKAGAFENLKVTPEKSARQASQEVKEDTKEDKVPFYKKH
                     STLLYASLLITFGYLSSYVNGEENIVTTLLFLASMFIGGLSLFKVGLQNLLRFEFDMK
                     TLMTVAVIGGAIIGEWAEVAIVVILFAISEALERFSMDRARQSIRSLMDIAPKEALVK
                     RNGQEIMIHVDDIAVGDIMIVKPGQKIAMDGVVVSGYSAVNQTAITGESVPVEKTVDN
                     EVFAGTLNEEGLLEVEITKLVEDTTISKIIHLVEEAQGERAPSQAFVDKFAKYYTPII
                     MIIATLVAIVPPLFFDGSWETWIYQGLAVLVVGCPCALVISTPISIVSAIGNAAKKGV
                     LVKGGVYLEEMGALKAIAFDKTGTLTKGVPAVTDYNVLNKQINEKELLSIITALEYRS
                     QHPLASAIMKKAEEENITYSDVQVEDFSSITGKGIKGIVNGTTYYIGSPKLFKELLTN
                     DFDKDLEQNVTTLQNQGKTAMIIGTEKEILAVIAVADEVRESSKEILQKLHQLGIKKT
                     IMLTGDNKGTANAIGGQVGVSDIEAELMPQDKLDFIKQLRSEYGNVAMVGDGVNDAPA
                     LAASTVGIAMGGAGTDTALETADVALMGDDLRKLPSTVKLSRKTLNIIKANITFAIAI
                     KFIASLLVIPGWLTLWIAILSDMGATLLVALNGLRLMRVKE"
                     /citation=[1]
BASE COUNT      930 a    422 c    588 g    809 t
ORIGIN
        1 cgttaatggg aattactatt agaaaaatca ttttatgttt ttcattcaaa tattcacttg
       61 aatattaacc aaggaaaggt atatactata ttcaagtgaa cacttgaata tcaatggggt
      121 gataatgtga ataagaaaga tacttgtgaa attttttgtt atgacgagga aaaggtcaat
      181 cgaatacaag gtgatttaaa aacaatcgat attgttagtg tcgcccaaat gttaaaagca
      241 attgcggatg aaaatagggc aaagattacc tatgctttgt gtcaagatga agagtcatgt
      301 gtgtgtgata tagcaaatat tattggtatt acggcggcaa atgcttctca tcatttaagg
      361 acccttcata agcaggggat tgtcagatat agaaaagaag gaaaactagc gttttattcg
      421 ttagacgatg aacatattag acaaataatg atgattgtac tagaacataa gaaagaagtg
      481 aatgtcaatg tctgatcaaa aggcaataac atctgaacaa gaaatgaagg cctatcgtgt
      541 acaaggtttt acttgcgcaa actgcgcggg taaattcgaa aaaaatgtaa aacagctatc
      601 tggagttgag gatgcaaaag taaattttgg tgcatctaaa attgctgttt atggtaatgc
      661 aacgatagaa gaactagaaa aagcaggtgc atttgagaat ctcaaagtga cacctgaaaa
      721 atctgctcgc caagcatcac aagaggtaaa agaagatacg aaagaagata aagtgccgtt
      781 ttataaaaag catagtactc tactttatgc ttccttattg attacctttg gttatctttc
      841 ctcgtatgtg aatggagaag agaacattgt tactacctta ttgtttttag catccatgtt
      901 tatcggagga ttatcacttt ttaaagttgg tttgcaaaac ttactacgtt ttgaatttga
      961 tatgaaaacc cttatgacag tggctgttat aggtggtgct attattggag aatgggcaga
     1021 agttgccatt gttgttattc tctttgcaat cagtgaagca cttgagcgat tctctatgga
     1081 tagagcaaga caatcgattc gttcattaat ggatatcgct cctaaagagg cacttgttaa
     1141 acgtaatgga caagaaataa tgattcatgt tgatgatatt gctgttgggg atatcatgat
     1201 tgtaaaacct ggtcaaaaga ttgcgatgga tggtgtagtt gtaagtgggt actctgccgt
     1261 taaccaaaca gctattacag gtgaatcagt ccctgtcgag aaaacggttg ataatgaagt
     1321 atttgcaggt accttaaatg aagaaggatt acttgaagta gaaataacga aacttgtaga
     1381 agatacaaca atttctaaaa ttattcatct tgtagaggaa gcacaagggg aacgtgctcc
     1441 ttcgcaagca tttgttgata aattcgcaaa atattacaca ccgattatta tgatcattgc
     1501 aacattagtt gctatagtcc ctccattatt ctttgatggc agttgggaaa catggattta
     1561 tcaaggatta gctgttcttg ttgttggttg tccttgtgcg ttggttatat cgactcctat
     1621 ttctattgtt tcagcgattg gaaatgctgc gaaaaaaggg gtccttgtaa aaggcggagt
     1681 gtatttagaa gaaatgggcg ccttaaaggc tattgcattc gataaaacag gaacattaac
     1741 aaaaggggtc ccagctgtaa ctgattataa tgtgttgaac aaacagataa atgaaaaaga
     1801 attactatcc attattactg cattggagta tcgttctcag catcctcttg cttcagccat
     1861 catgaaaaaa gcagaagaag aaaacattac ttattctgac gtacaggtag aggatttctc
     1921 ttctatcaca ggaaagggta taaagggtat cgtaaacggg acgacttatt acatcggtag
     1981 cccgaaactc tttaaggaat tattgactaa tgatttcgat aaagacttag agcaaaatgt
     2041 tactactctt caaaatcaag gtaagacagc catgattatt ggaaccgaaa aagaaatact
     2101 tgcagttatt gcagttgctg atgaagttcg tgagtcaagt aaggaaattc ttcaaaagtt
     2161 acatcaactt ggaatcaaaa aaacaattat gcttactggt gataacaaag gtactgcgaa
     2221 tgctatcgga gggcaggttg gagtatctga tatagaggca gaattaatgc cacaggacaa
     2281 attagacttt attaaacagt tgagatccga gtatggcaac gtagcaatgg taggagatgg
     2341 ggtcaatgat gcacctgcat tggcggcctc aacagttggg attgcaatgg gtggagctgg
     2401 tacagacaca gccttagaaa ctgcagacgt cgctctaatg ggagacgatt taagaaaact
     2461 tccatccact gtaaaactta gccggaaaac tcttaatatc atcaaagcta acatcacttt
     2521 tgcaattgct attaaattca ttgcctcact attggttatc ccaggttggt taacgctctg
     2581 gattgccatt ctttccgata tgggcgcaac ccttctagta gctctaaatg gcttgcgact
     2641 aatgagagtg aaagaataaa ggaaaaagtg ctaaaaaggg ctcttccaga cagatggaaa
     2701 ttcctttttt aaaaaggact cattattagt ataacttcta aatataata
//