From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: M.musculus mRNA for epithelin 1 and 2
Date: 11 Sep 92 10:05:01 GMT
Distribution: bionet
Checksum: 51946     6

LOCUS       MUSEPIT1     2100 bp ss-RNA             ROD       11-SEP-1992
DEFINITION  M.musculus mRNA for epithelin 1 and 2
ACCESSION   X62321
KEYWORDS    Epithelin 1; Epithelin 2; epithelial cell growth regulator;
            soluble protein.
SOURCE      Mus musculus RNA.
  ORGANISM  Mus musculus
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Rodentia; Myomorpha; Muridae; Murinae.
REFERENCE   1  (bases 1 to 2100)
  AUTHORS   Plowman,G.D.
  JOURNAL   Unpublished (1991)
  STANDARD  full automatic
REFERENCE   2  (sites)
  AUTHORS   Plowman,G.D., Green,J.M., Neubauer,M.G., Buckley,S.D.,
            McDonald,V.L., Todaro,G.J. and Shoyab,M.
  TITLE     The epithelin precursor encodes teo proteins with opposing
            activities on epithelial cell growth
  JOURNAL   J. Biol. Chem. 267, 13073-13078 (1992)
  STANDARD  full staff_review
COMMENT     *source: tissue=kidney; *source: cell_line=kidney T cell genomic;
            *source: clone_library=lambda FIXII, PCR clones in Bluescript;
            See also X62320-2
            From EMBL    entry MMEPIT1;  dated 26-AUG-1992.
FEATURES             Location/Qualifiers
     sig_peptide     8..58
     CDS             8..1777
                     /product="Epithelin 1 & 2"
                     /codon_start=1
                     /translation="MWVLMSWLAFAAGLVAGTQCPDGQFCPVACCLDQGGANYSCCNP
                     LLDTWPRITSHHLDGSCQTHGHCPAGYSCLLTVSGTSSCCPFSKGVSCGDGYHCCPQG
                     FHCSADGKSCFQMSDNPLGAVQCPGSQFECPDSATCCIMVDGSWGCCPMPQASCCEDR
                     VHCCPHGASCDLVHTRCVSPTGTHTLLKKFPAQKTNRAVSLPFSVVCPDAKTQCPDDS
                     TCCELPTGKYGCCPMPNAICCSDHLHCCPQDTVCDLIQSKCLSKNYTTDLLTKLPGYP
                     VKEVKCDMEVSCPEGYTCCRLNTGAWGCCPFAKAVCCEDHIHCCPAGFQCHTEKGTCE
                     MGILQVPWMKKVIAPRRLPDPQILKSDTPCDDFTRCPTNNTCCKLNSGDWGCCPIPEA
                     VCCSDNQHCCPQGFTCLAQGYCQKGDTMVAGLEKIPARQTTPLQIGDIGCDQHTSCPV
                     GQTCCPSLKGSWACCQLPHAVCCEDRQHCCPAGYTCNVKARTCEKDVDFIQPPVLLTL
                     GPKVGNVECGEGHFCHDNQTCCKDSAGVWACCPYLKGVCCRDGRHCCPGGFHCSARGT
                     KCLRKKIPRWDMFLRDPVPRPLL"
     mat_peptide     59..1774
                     /product="Epithelin 1 & 2"
     misc_feature    119..121
                     /note="pot. glycosylation site"
     misc_feature    182..349
                     /note="cysteine-motif 1"
     misc_feature    374..544
                     /note="cysteine-motif 2"
     misc_feature    620..790
                     /note="Epithelin 2"
     misc_feature    845..1012
                     /note="Epithelin 1"
     misc_feature    1091..1255
                     /note="cysteine-motif 5"
     misc_feature    1124..1126
                     /note="pot. glycosylation site"
     misc_feature    1325..1492
                     /note="cysteine-motif 6"
     misc_feature    1550..1717
                     /note="cysteine-motif 7"
     misc_feature    1583..1585
                     /note="pot. glycosylation site"
BASE COUNT      430 a    602 c    571 g    497 t
ORIGIN
        1 gcagaccatg tgggtcctga tgagctggct ggccttcgcg gcagggctgg tagccggaac
       61 acagtgtcca gatgggcagt tctgccctgt tgcctgctgc cttgaccagg gaggagccaa
      121 ctacagctgc tgtaaccctc ttctggacac atggcctaga ataacgagcc atcatctaga
      181 tggctcctgc cagacccatg gccactgtcc tgctggctat tcttgtcttc tcactgtgtc
      241 tgggacttcc agctgctgcc cgttctctaa gggtgtgtct tgtggtgatg gctaccactg
      301 ctgcccccag ggcttccact gtagtgcaga tgggaaatcc tgcttccaga tgtcagataa
      361 ccccttgggt gctgtccagt gtcctgggag ccagtttgaa tgtcctgact ctgccacctg
      421 ctgcattatg gttgatggtt cgtggggatg ttgtcccatg ccccaggcct cttgctgtga
      481 agacagagtg cattgctgtc cccatggggc ctcctgtgac ctggttcaca cacgatgcgt
      541 ttcacccacg ggcacccaca ccctactaaa gaagttccct gcacaaaaga ccaacagggc
      601 agtgtctttg cctttttctg tcgtgtgccc tgatgctaag acccagtgtc ccgatgattc
      661 tacctgctgt gagctaccca ctgggaagta tggctgctgt ccaatgccca atgccatctg
      721 ctgttccgac cacctgcact gctgccccca ggacactgta tgtgacctga tccagagtaa
      781 gtgcctatcc aagaactaca ccacggatct cctgaccaag ctgcctggat acccagtgaa
      841 ggaggtgaag tgcgacatgg aggtgagctg ccctgaagga tatacctgct gccgcctcaa
      901 cactggggcc tggggctgct gtccatttgc caaggccgtg tgttgtgagg atcacattca
      961 ttgctgcccg gcagggtttc agtgtcacac agagaaagga acctgcgaaa tgggtatcct
     1021 ccaagtaccc tggatgaaga aggtcatagc cccccgccgc ctgccagacc cacagatctt
     1081 gaagagtgat acaccttgtg atgacttcac taggtgtcct acaaacaata cctgctgcaa
     1141 actcaattct ggggactggg gctgctgtcc catcccagag gctgtctgct gctcagacaa
     1201 ccagcattgc tgccctcagg gcttcacatg tctggctcag gggtactgtc agaagggaga
     1261 cacaatggtg gctggcctgg agaagatacc tgcccgccag acaaccccgc tccaaattgg
     1321 agatatcggt tgtgaccagc ataccagctg cccagtaggg caaacctgct gcccaagcct
     1381 caagggaagt tgggcctgct gccagctgcc ccatgctgtg tgctgtgagg accggcagca
     1441 ctgttgcccg gccgggtaca cctgcaatgt gaaggcgagg acctgtgaga aggatgtcga
     1501 ttttatccag cctcccgtgc tcctgaccct cggccctaag gttgggaatg tggagtgtgg
     1561 agaagggcat ttctgccatg ataaccagac ctgttgtaaa gacagtgcag gagtctgggc
     1621 ctgctgtccc tacctaaagg gtgtctgctg tagagatgga cgtcactgtt gccccggtgg
     1681 cttccactgt tcagccaggg gaaccaagtg tttgcgaaag aagattcctc gctgggacat
     1741 gtttttgagg gatccggtcc caagaccgct actgtaagga agggctacag acttaaggaa
     1801 ctccacagtc ctgggaaccc tgttccgagg gtacccacta ctcaggcctc cctagcgcct
     1861 cctcccctaa cgtctccccg gcctactcat cctgagtcac cctatcacca taaaaggtgg
     1921 agcctcaaac taaaaccttc ttttatggaa agaaggctgt ggccaaaagc cccgtatcaa
     1981 actgccattt cttccggttt ctgtggacct tgtggccagg tgctcttccc gagccacagg
     2041 tgttctgtga gcttgcttgt gtgtgtgtgc gcgtgtgcgt gtgttgctcc aataaagttt
//