From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: P.sativum lpd mRNA for dihydrolipoamide dehydrogenase
Date: 10 Apr 92 10:10:29 GMT
Distribution: bionet
Checksum: 15889     5

LOCUS       PEALPD       1627 bp ss-mRNA            PLN       10-APR-1992
DEFINITION  P.sativum lpd mRNA for dihydrolipoamide dehydrogenase
ACCESSION   X63464
KEYWORDS    E3 protein; L protein; dihydrolipoamide dehydrogenase; lpd gene;
            oxido-reductase.
SOURCE      Pisum sativum RNA.
  ORGANISM  Pisum sativum
            Eukaryota; Plantae; Embryobionta; Magnoliophyta; Magnoliopsida;
            Rosidae; Rosales; Fabaceaea.
REFERENCE   1  (bases 1 to 1627)
  AUTHORS   Bourguignon,J., Macherel,D., Neuburger,M. and Douce,R.
  TITLE     Isolation characterization and sequence analysis of a cDNA clone
            encoding L-protein, the dihydrolipoamide dehydrogenase component of
            the glycine cleavege system from pea leaf mitochondria
  JOURNAL   Eur. J. Biochem. 204, 865-873 (1992)
  STANDARD  full automatic
COMMENT     *source: tissue=leaf; *source: clone_library=lambda gt11;
            From EMBL    entry PSLPD;  dated 20-MAR-1992.
FEATURES             Location/Qualifiers
     sig_peptide     34..126
     CDS             34..1539
                     /EC_number="1.8.1.4"
                     /product="dihydrolipoamide dehydrogenase"
                     /gene="lpd"
                     /translation="MAMANLARRKGYSLLSSETLRYSFSLRSRAFASGSDENDVVIIG
                     GGPGGYVAAIKAAQLGFKTTCIEKRGALGGTCLNVGCIPSKALLHSSHMYHEAKHSFA
                     NHGVKVSNVEIDLAAMMGQKDKAVSNLTRGIEGLFKKNKVTYVKGYGKFVSPSEISVD
                     TIEGENTVVKGKHIIIATGSDVKSLPGVTIDEKKIVSSTGALALSEIPKKLVVIGAGY
                     IGLEMGSVWGRIGSEVTVVEFASEIVPTMDAEIRKQFQRSLEKQGMKFKLKTKVVGVD
                     TSGDGVKLTVEPSAGGEQTIIEADVVLVSAGRTPFTSGLNLDKIGVETDKLGRILVNE
                     RFSTNVSGVYAIGDVIPGPMLAHKAEEDGVACVEYLAGKVGHVDYDKVPGVVYTNPEV
                     ASVGKTEEQVKETGVEYRVGKFPFMANSRAKAIDNAEGLVKIIAEKETDKILGVHIMA
                     PNAGELIHEAAIALQYDASSEDIARVCHANPTMSEAIKEAAMATYDKPIHI"
     mat_peptide     127..1536
                     /EC_number="1.8.1.4"
                     /product="dihydrolipoamide dehydrogenase"
                     /gene="lpd"
BASE COUNT      454 a    306 c    413 g    454 t
ORIGIN
        1 aaaaagttag cgttaagtga tcgaatcgaa aaaatggcta tggcgaactt ggctcgtcgg
       61 aagggttact ctcttctctc atcggagact ctccgctact ctttttctct caggtcaaga
      121 gctttcgcct ccggatctga tgaaaacgac gtcgtcatca tcggtggtgg tcccggcggt
      181 tacgtcgccg ccattaaagc cgctcagctt ggtttcaaaa ctacttgcat cgagaagcgt
      241 ggcgccctcg gtggtacttg tctcaacgtt ggatgcatcc cttccaaggc acttttgcat
      301 tcttcgcata tgtaccatga ggctaaacat tcatttgcca accatggtgt taaagtttca
      361 aatgtggaga ttgacttggc tgccatgatg gggcaaaaag ataaagctgt ttctaatctt
      421 acccggggta ttgaaggcct atttaagaag aataaggtaa cctatgttaa aggatatgga
      481 aaatttgttt cgccgtctga aatctctgta gacaccattg aaggtgaaaa tactgtggtt
      541 aagggaaagc atatcattat tgctactggt tcagatgtca aatctctccc cggtgtcact
      601 attgatgaaa agaaaattgt atcatcgact ggggctctcg cattgtctga aatccctaag
      661 aagcttgtag ttattggggc aggttacatt gggctggaaa tgggctcagt gtggggccga
      721 attgggtctg aggtaactgt tgttgagttt gcatcagaga ttgttccaac catggacgca
      781 gaaatccgga agcagtttca gcgttctctt gaaaagcaag gcatgaaatt caaactgaag
      841 accaaggtgg ttggagttga tacttctggg gatggtgtga agctaaccgt tgaaccatcc
      901 gctggcggtg aacagaccat aattgaagca gatgttgtcc ttgtttcggc tggtagaact
      961 ccattcactt ctggacttaa tttggataag ataggagttg agactgacaa gttaggacgg
     1021 attttggtaa atgaaagatt ttcaacaaat gtctctggtg tctatgcaat cggagatgtg
     1081 attccaggtc caatgttggc acacaaggca gaagaagatg gagttgcttg tgtcgagtac
     1141 ttagccggta aggttggcca tgtggactat gacaaagtac caggtgttgt ctatacaaac
     1201 cctgaagttg catctgtagg gaagacagag gagcaggtta aggaaactgg agttgaatac
     1261 cgtgttggaa agttcccttt catggctaat agcagggcaa aggcaattga taacgctgaa
     1321 ggactagtca agataattgc tgaaaaggag acagacaaaa tattgggagt acatattatg
     1381 gcacctaatg caggagaact cattcatgaa gcagccatag cattgcagta tgatgcatca
     1441 agtgaggaca tagcacgtgt gtgccatgcc aatccaacaa tgagcgaagc tattaaagaa
     1501 gctgcaatgg caacatatga caagcccatt cacatttgaa gagctggttg cttctttctt
     1561 ctcttgtttt catttttgtc caaggattta ccttgacttg gagaagatac tttgtgtgtt
     1621 aaactta
//