From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Choristoneura fumiferana nuclear polyhedrosis virus immediate-early
Date: 5 Nov 92 23:12:50 GMT
Distribution: bionet
Checksum: 37897 10

LOCUS       NPHIE1A      4179 bp ds-DNA             VRL       14-OCT-1992
DEFINITION  Choristoneura fumiferana nuclear polyhedrosis virus immediate-early
            protein 1 (IE1) gene and unidentified orf, complete coding regions
            and enhancers.
ACCESSION   L04945
KEYWORDS    enhancer; immediate-early protein; inverted repeat.
SOURCE      Choristoneura fumiferana nuclear polyhedrosis virus (strain HR3)
            DNA.
  ORGANISM  Choristoneura fumiferana nuclear polyhedrosis virus
            Viridae; ds-DNA enveloped viruses; Baculoviridae; Baculovirus.
REFERENCE   1  (bases 1 to 4179)
  AUTHORS   Kuzio,J., Schodella,E. and Faulkner,P.
  TITLE     Sequence and functional analysis of Choristoneura fumiferana
            Nuclear Polyhedrosis Virus IE1 gene and HR1 enhancer element
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     promoter        complement(41..45)
                     /standard_name="ATAAG box; baculovirus late promoter"
                     /gene="IE1"
                     /note="putative"
     promoter        complement(51..55)
                     /standard_name="ATAAG box; baculovirus late promoter"
                     /gene="IE1"
                     /note="putative"
     TATA_signal     69..76
                     /gene="IE1"
                     /note="putative"
     mRNA            100..>1817
                     /note="cap site: CAGT"
                     /gene="IE1"
                     /note="putative"
     enhancer        3099..3122
                     /standard_name="baculovirus core enhancer element"
                     /note="one of 11 24 basepair imperfect palindromic
                     sequences in the HR1 region; these inverted repeats may be
                     the core sequences of baculovirus enhancers"
                     /note="putative"
     enhancer        3200..3223
                     /note="inverted repeat"
                     /note="putative"
     enhancer        3259..3282
                     /note="inverted repeat"
                     /note="putative"
     enhancer        3325..3348
                     /note="inverted repeat"
                     /note="putative"
     enhancer        3510..3533
                     /note="inverted repeat"
                     /note="putative"
     enhancer        3572..3595
                     /note="inverted repeat"
                     /note="putative"
     enhancer        3682..3705
                     /note="inverted repeat"
                     /note="putative"
     enhancer        3755..3788
                     /note="inverted repeat"
                     /note="putative"
     enhancer        3811..3834
                     /note="inverted repeat"
                     /note="putative"
     enhancer        3873..3896
                     /note="inverted repeat"
                     /note="putative"
     enhancer        3933..3956
                     /note="inverted repeat"
                     /note="putative"
     CDS             138..1820
                     /standard_name="IE1"
                     /evidence="EXPERIMENTAL"
                     /product="immediate-early protein 1"
                     /codon_start=1
                     /translation="MPKNMAALQQSLYTGPSTPSHTQFSRSTEFPENLNFDVLNDSYE
                     TFSSVSLTTAEQDNQIDKILQESAAMNRDVNSELAQFTASEYVTGFRADTMEPEVIVE
                     TIGDSMKRKASELDSDSDSGESSKGKKRVIKPKMRQRYKKATIQNKTSLTEECNYNTE
                     ICTVAPTDQIAEYFKHDFSVYLEKQKSDCQMSANRFSDYISETGYYVFVVKKSEHKPF
                     EVVFAKFVNNVTNEYTNNYYMVDNRVFVVSLNNVKFMVSYKLVREQGIDIPPHVNLCD
                     DAQAERNPYDCYFEPVKNVFQTTLINHFHLDMYYSQTTFVTLMQSMGESKSGMLLNKL
                     YQMFQDRSLFTLPIMLSRKEPTIENTPLSRNYTSSYVAQIIKYSKNVRFPENNPDNGV
                     ISRLEEIVTQKSSLTYKYSSVANLLFSRYGHQRDNNADSLKKVKKEDGNRLLVEQYMS
                     QNENDETSHNFIVLQFGGVNDERLTIAKKGIEFFWIAAEIKDINVDDLVKKYTRNVHH
                     VFRIINVNRRESTTWHNNLLKLLQLLLQNLIRIDDVQQYSNKGDSKFIYKRL"
     CDS             complement(1893..3032)
                     /standard_name="ORF 1137"
                     /note="product unidentified"
                     /note="putative"
                     /product="ORF 1137"
                     /codon_start=1
                     /translation="MSTFFTNLRRVNKVYPNQATFLTDNTRLLTTTPAGFTNVLRAPS
                     TRNLGNGRFEPGYNLSNNQFVSAGDINRITRGNDVPRIRNVFQGISDPQIGSLNQLRR
                     ADNVPDAGLHVKRTRSDAVKQNFPETNVRSADGVDRALQQNPRLNTYLQGAKTAGVGV
                     LLAGGAYLTFSAATLVQDIIQALNNTGGSYYVRGADGGDTADACLLLSRTCQRDPNMN
                     TSDVVICNHDPLIADTAQLQAICSGFNYQQEQTVCRQSDPAADPDSPQFVDVSDLLPG
                     QTIMCIEPYNLGDLIGDLGLDHLLGEDGLVGKSSNSSDSVSNKLMPLIWLIGAVLFLG
                     LIIYLIYRFVIKGGAGAAGAARAPPVIVLPPPPTQQTYNSTKQQI"
BASE COUNT     1062 a    974 c   1082 g   1061 t
ORIGIN      
        1 cgataacgcc gttagtggcg tgtggcatgt agaacctaat atcgttatct tgttcaccat
       61 cgggttggta taaatagacg ttcatgttgg tttttgtttc agttgcaagt tgaccgcgac
      121 gcgcgcagta cctcgcaatg cctaagaata tggccgcttt acaacagtct ctgtacacgg
      181 gcccgtccac gccaagccac acgcagttca gcaggtccac cgaatttcct gaaaatttga
      241 actttgacgt cttaaacgat tcttatgaaa cattctcctc tgtaagcttg acaacagccg
      301 aacaagataa ccaaattgat aagattcttc aagaatccgc agctatgaat cgcgatgtca
      361 atagtgagct ggcgcagttt actgcgtcgg agtatgtgac cggttttcgc gccgatacga
      421 tggagcccga agtgatcgtg gaaacaattg gagacagcat gaaacgcaaa gcgtctgagc
      481 tggacagcga ctccgacagc ggggagtctt caaaaggcaa gaaacgcgtc atcaagccca
      541 aaatgcgcca gcgctacaag aaagccacca ttcagaacaa aacttctttg actgaagagt
      601 gcaattataa taccgagatt tgcaccgtgg cgcctacgga ccaaattgcg gaatatttta
      661 aacatgactt ttctgtgtac ctggagaagc agaaaagcga ttgtcaaatg agcgccaacc
      721 ggttttcgga ttacatttcc gagactggct attacgtgtt tgtcgttaag aaaagcgaac
      781 ataagccgtt tgaagtggtg tttgcaaagt ttgtcaacaa cgtgactaac gagtacacta
      841 ataactatta tatggtagac aatcgcgtgt ttgtggtgtc gctgaataac gtgaagttta
      901 tggtgtcgta taagctagtg cgcgagcagg gcatcgatat tccgccgcac gtgaacctgt
      961 gtgacgacgc gcaggcagag cgcaacccct acgattgcta ttttgagccg gtcaaaaatg
     1021 tgttccaaac cacgctgatc aaccattttc acttggacat gtactattct caaaccacct
     1081 ttgttacgct catgcagtcc atgggcgaga gcaagagcgg tatgctgctg aacaagctgt
     1141 atcaaatgtt ccaagaccgt tccctattca cgcttcccat aatgttgagc cgcaaggagc
     1201 ccactattga gaacacgccg ctcagcagga attacacgtc ttcttacgtg gcgcagatca
     1261 tcaaatattc caaaaacgtg cgctttcccg aaaacaatcc ggacaacggc gttattagtc
     1321 gactagagga gatcgtcact cagaaatcat cgctgactta caagtacagt agcgtggcca
     1381 atctgttgtt cagcagatac ggtcatcagc gtgacaataa cgcggacagc ttaaagaagg
     1441 tcaaaaagga agacggcaac cggctgctgg tggaacagta catgtcgcaa aacgagaacg
     1501 acgagaccag tcacaatttt attgtgctgc agttcggagg cgtcaacgac gagcgcctga
     1561 ccattgccaa aaagggaatt gaatttttct ggatcgctgc cgaaataaaa gacatcaacg
     1621 tggacgattt ggtcaaaaaa tatactcgta acgtgcacca cgtgtttaga attataaatg
     1681 taaaccggcg cgaaagcacc acgtggcaca ataacctgtt gaaattgcta cagctgttgt
     1741 tgcaaaattt aattagaata gacgacgtgc agcaatattc taacaaaggt gatagtaaat
     1801 ttatctataa aagactgtaa tatacacgat actcaacaaa agtgatagta gatttatttt
     1861 tgaaagttat tgtaaaaaat aaatatttgc atttaaatct gttgttttgt gctattatac
     1921 gtttgttgtg tgggcggggg cggtaatacg atgactggcg gggcgcgggc cgcgcccgct
     1981 gccccggcgc cgcctttaat tacgaatctg taaattagat aaatgatgag acctaaaaat
     2041 aacacggcgc cgatcagcca aatgagaggc ataagcttgt tgctgacgct gtcgctagaa
     2101 tttgaagact tgcccactaa tccgtcttcg cctaacaaat gatccaagcc caagtcgcca
     2161 attaggtcgc ccaggttgta cggctcaatg cacattatgg tctggccggg tagcaggtcg
     2221 ctaacgtcca caaactgcgg cgagtcgggg tctgcggcgg ggtcgctctg ccggcacacc
     2281 gtttgctcct gctggtagtt gaacccgctg cagatggctt gcagctgcgc cgtgtcggcg
     2341 ataagcgggt catggttaca aataaccacg tcggacgtgt tcatgttggg gtcacgctgg
     2401 cacgtgcggc tgagcagcag gcacgcgtcg gccgtgtcgc cgccgtcggc gccgcgcaca
     2461 tagtaactgc cgccagtgtt gttgagcgct tgaattatgt cctgcactag cgtcgccgcg
     2521 ctaaaggtga ggtaagcgcc gccggccagc aacacgccca cgccggccgt tttggcgccc
     2581 tgcagatagg tgttaaggcg cgggttctgc tgcagcgcgc ggtccacgcc gtcggccgag
     2641 cgcacgttgg tttcggggaa gttttgctta accgcatcgc tgcgcgtgcg cttcacgtgc
     2701 aggcccgcgt cgggcacgtt gtcggcgcgc cgtagctggt ttagcgaccc gatttgcggg
     2761 tcgctgatgc cctggaacac gttacggatg cgcggcacat cattgccgcg cgtaatgcgg
     2821 ttgatgtcgc ccgcgctcac gaactgattg ttggacaggt tgtagccagg ttcaaaacgg
     2881 ccgttgccca agttacgcgt gctgggcgct ctgagcacat ttgtgaaccc cgccggtgta
     2941 gttgtcagca ggcgcgtgtt gtcggtcaag aaggtggctt gattggggta cactttgtta
     3001 accctgcgca gatttgtaaa gaaagtgctc atgtttgcga cttaatatga cttaagtttc
     3061 aatgcaattt aattattggc atcagcattg accccgcttt tcaagtgcga gcgttgtcaa
     3121 aaacaagcgt tattaataac gtgcgtcagc gccgaccttg cttttcaagt acgagaaaac
     3181 aagcagcgcc gaccttgctt ttcgagtacg attgttgttg aaaaactagt gttatcttta
     3241 gacggcgccg acctcgcttt tcgagtacga ttgttgttga aaaacaagtg ctatctttag
     3301 acgtgcgtca gcgccgacct cgcttttcgg gtacgattgt tgttgagaaa actagtgtaa
     3361 taggttaaat cgtgcgtctg gttttgatga cgtgcgtcgc gtgcgtcagc gccgacctcg
     3421 cttttcgggt acgattgtta gttttatctt tagacgtgcg tcggcgccga ccttgctttt
     3481 tgatacgatt gaaaagcgcc gacctcgctt ttcgggaacg ggtgttctcg aaaagcgagt
     3541 gctatagacg tgcgtcagcg ccgacctcgc ttttcgggta cgggtgttct cgaaaagcga
     3601 gtgctataga cgtgcgtcag cgccgacctc gcttttctcg aaaagcgagt gctataaacg
     3661 tgcgtcagcg ccgacctcgc ttttcgggta cgggtgttct cgaaaaacaa gtgctatctt
     3721 tagactgcgt cggcgcctgt ctgaggcatt cgtttttcga gaacatctgt actcgaaaag
     3781 tggagtcggc gctgacgcat gtctggcatt tatttcgaac atctgtactt ggaaagcggg
     3841 atcggcgttg acgcatgtct aagggatttg ttttttgaga acatctgtac ttggaaagcg
     3901 gggtcggcgc caccaacacg tgactaaaat atttttaaac acgtccgtac acgtaaacgt
     3961 atggcactcg tacccgaaaa gcggggttgg cgccgacgca tgaaatgtat cgacaaaagc
     4021 atttgccgtt gacagttgtt ttttagggtt gcacaggtat ataaagacaa acagtttatt
     4081 aatacagttt attaatatat aaaacaatgt taatatcaat cgcataaatt ccattaggtt
     4141 tgtttgctga cgcaaaaact cttgcacgta agtaagctt
//