From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human lymphocyte surface protein exons 1-5, complete cds.
Date: 21 Dec 92 20:46:45 GMT
Distribution: bionet
Checksum: 06374 7

LOCUS       HUMLSPRO     2622 bp ss-mRNA            PRI       20-NOV-1992
DEFINITION  Human lymphocyte surface protein exons 1-5, complete cds.
ACCESSION   M99578
KEYWORDS    lymphocyte surface protein.
SOURCE      Homo sapiens (library: lambda gt11) neonate placenta cDNA to mRNA.
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 2622)
  AUTHORS   Voland,J.R., Wyzykowski,R.J., Mark,H. and Dutton,R.W.
  TITLE     Cloning and sequencing of a trophoblast- endothelial- activated
            lymphocyte surface protein: cDNA sequence and genomic structure
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. (1992) In press
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     mRNA            join(1..153,153..933,934..1082,1083..1323,1324..2622)
     exon            1..153
                     /number=1
                     /note="codes the 5' UTR"
                     /note="intron between exon 1 and exon 2 is 2.7Kb"
     5'UTR           1..171
                     /note="84% GC in 5'UTR"
     exon            153..933
                     /number=2
                     /note="codes the extracellular domain"
                     /note="intron between exon 2 and exon 3 is 0.7Kb"
     misc_feature    172..232
                     /note="codes for protein leader sequence"
     exon            934..1082
                     /number=3
                     /note="codes the putative basic region"
                     /note="intron between exon 3 and exon 4 is 3.5Kb"
     exon            1083..1323
                     /number=4
                     /note="codes the leucine zipper region"
                     /note="the intron between exon 4 and exon 5 is 1.3Kb"
     misc_feature    1199..1263
                     /note="codes for leucine zipper, homologous to myc"
     exon            1324..2622
                     /number=5
                     /note="codes the serine phosphorylation site and the
                     3'UTR"
     CDS             170..1822
                     /note="550 amino acids MW=61kDa, glycosylated=75 kDa"
                     /note="expressed on endothelium, activated lymphocytes and
                     syncytiotrophoblast, contains leucine zipper and basic
                     region homologous to myc"
                     /note="721P"
                     /codon_start=1
                     /translation="MAAATIVHDTSEAVELCPAYGLYLKPITKMTISVALPQLKQPGK
                     SISNWEVMERLKGMVQNHQFSTLRISKSTMDFIRFEGEVENKSLVKSFLACLDGKTIK
                     LSGFSDILKVRAAEFKIDFPTRHDWDSFFRDAKDMNETLPGERPDTIHLEGLPCKWFA
                     LKESGSEKPSEDVLVKVFEKFGEIRNVDIPMLDPYREEMTGRNFHTFSFGGHLNFEAY
                     VQYREYMGFIQAMSALRGMKLMYKGEDGKAVACNIKVSFDSTKHLSDASIKKRQLERQ
                     KLQELEQQREEQKRREKEAEERQRAEERKQKELEELERERKREEKLRKREQKQRDREL
                     RRNQKKLEKLQAEEQKQLQEKIKLEERKLLLAQRNLQSIRLIAELLSRAKAVKLREQE
                     QKEEKLRLQQQEERRRLQEAELRRVEEEKERALGLQRKERELRERLLSILQSKKPDDS
                     HTHDELGVAHGPAAARPGHPADRVVRLCERHHAAPPRGPAPGRCPQGEPGPPRGRRRS
                     QKRERERGRGGPMQGGSELLSCGPRGWLSREEVPGRRPLLHS"
BASE COUNT      589 a    792 c    898 g    343 t
ORIGIN      
        1 ggcgacggcg gtggcggcgt cggaggcgcc tccgggggac ggtggcggct cccggcggtg
       61 aggccgcgcc tgtccgggga tcgtcgaggg acggcgggag cttgggccag cggcggcggc
      121 ggcctgggac gcaggcggag ccccgcgcag gcccaaggtc ccggaggcta tggcagcggc
      181 taccatcgtg cacgacacgt ctgaggccgt ggagctctgc cctgcttacg gcttgtacct
      241 gaagcccatc accaagatga ccatcagcgt ggcactcccg cagctgaagc agccggggaa
      301 gtccatctcc aactgggagg tgatggagag gctgaagggc atggtgcaga accaccagtt
      361 ctccacgctg cgtatttcca agagcaccat ggacttcatc cgcttcgagg gggaggtgga
      421 gaacaagagc ctggtcaagt cttttctggc ctgcctggac ggcaagacca tcaagctcag
      481 cggcttctcc gacatcctga aggtgcgcgc ggccgagttc aagatcgact tccccacccg
      541 ccacgactgg gactccttct tccgcgacgc caaggacatg aacgagaccc tgccggggga
      601 gcggccggac accatccacc tggaggggct gccctgcaag tggttcgccc tgaaggagtc
      661 gggctccgag aagcccagcg aggacgtcct ggtcaaggtg tttgagaagt tcggggagat
      721 ccggaatgtg gacatcccca tgctggaccc ctaccgggag gagatgacgg gccgcaactt
      781 ccacaccttc agtttcgggg ggcacttgaa cttcgaggcc tatgtgcagt accgtgagta
      841 catgggcttc atccaggcca tgagcgccct gcgcgggatg aaactcatgt acaagggcga
      901 ggacggcaag gccgtggcct gcaacatcaa ggtttctttt gattcgacca aacacctgag
      961 tgatgcctca attaagaagc ggcagctgga gaggcagaag cttcaggaac tggagcagca
     1021 aagagaagaa caaaagcgca gagagaagga agcggaggag aggcagcgag cggaggaaag
     1081 gaaacaaaag gagctggaag agctggagcg agagaggaaa agagaagaga agcttcgcaa
     1141 gagggagcag aagcagaggg accgtgagct gcgccggaat cagaagaagc tggagaagct
     1201 gcaggcggag gagcagaagc agctgcagga gaagatcaag ctggaggagc gcaagctgct
     1261 gctggcccag aggaacctgc agtccatccg gctcatcgcc gagctgctca gcagagccaa
     1321 ggctgtgaag ctacgggaac aggagcagaa ggaggagaag ctgaggctcc agcagcagga
     1381 ggagcggcgg cggctgcagg aggccgagct gcggcgcgtg gaggaggaga aggagcgcgc
     1441 gctgggcctg cagcggaaag agcgggagct gcgcgagcgg ctgctgagca tcctgcagag
     1501 caagaagccg gacgacagcc acacacacga cgagctgggc gtggcacacg gacctgctgc
     1561 agcccgtcct ggacatcctg cagaccgtgt cgtccggctg tgtgagcgcc accacgctgc
     1621 accccctcgg gggccagccc ccggccggtg cccccaagga gagcccggcc cccccagagg
     1681 ccgacggcgc tcccaaaagc gtgaacggga gcgtggccga ggaggcccca tgcaaggagg
     1741 ttcagagctc ctgtcgtgtg gtccccgagg atggctctcc agagaagagg tgcccgggcg
     1801 gcgtcctctc ctgcattcct gacaacaacc aacagcccaa gggcatccct gcctgcgagc
     1861 agaatgtctc cagaaaggac acccggtcag aacaggacaa gtgcaaccgg gagcccagca
     1921 agggccgggg ccgggccacc ggagacgggc ttgctgaccg gcacaagcgg gagaggagcc
     1981 gggccaggcg ggccagcagc agggaggacg ggaggccacg caaggagcgg cggccccaca
     2041 agaagcacgc ctacaaggat gacagccccc gccggcgcag cacgagcccg gaccacaccc
     2101 ggtcccggag gtcccacagc aaagacaggc accggaggga gcggagccgg gagcggaggg
     2161 gcagcgccag caggaagcac agccgccacc gccgccgaag cgagcggtcg cgctcccggt
     2221 ccccgagcag gcaccgcagt acctggaaca ggtaatgacg ggcacggcct ccccacggcc
     2281 tgtccgggaa agaccaggac ctgctcgagc ctcctggccg ctccttggcc gctctccgtc
     2341 cacccctgca aagccaagac ccttctgcag ccacgaatgt ccacggagcc cgccggcagg
     2401 aaggaagaca ccatgcttta gagatccatc tttctccact caccgcagcg tacttggcac
     2461 ttcagtttca aacacgtagt cctttaaaac ttgatccgat agctttaatg cggccggtcc
     2521 tctctcagtc aggaaaattg cacagaccga cagtcgtgag gatggcagag ctgctgcatt
     2581 cccccacacg gggatttctg tgtctgcttg gcgacctcct ac
//