From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: V. radiata mRNA for invertase, complete cds.
Date: 21 Jul 92 10:08:52 GMT
Distribution: bionet
Checksum: 62464     6

LOCUS       VIRINVA      2477 bp ss-mRNA            PLN       21-JUL-1992
DEFINITION  V. radiata mRNA for invertase, complete cds.
ACCESSION   D10265
KEYWORDS    beta-fructofuranosidase; invertase.
SOURCE      Vigna radiata seedling stem, cDNA to mRNA, clone VAI1.
  ORGANISM  Vigna radiata
            Eukaryota; Plantae; Embryobionta; Magnoliophyta; Magnoliopsida;
            Rosidae; Fabales; Fabaceae.
REFERENCE   1  (bases 1 to 2477)
  AUTHORS   Imaseki,H., Mori,H. and Arai,M.
  TITLE     Cloning and sequence of cDNAs for an intracellular acid invertase
            from etiolated hypocotyls of mung bean and expression of the gene
            during growth of seedlings
  JOURNAL   Plant Cell Physiol. (1992) In press
  STANDARD  full staff_review
COMMENT     Submitted (21-Jan-1992) to DDBJ by:
            Masao Arai
            National Institute for Basic Biology
            Myodaiji-cho, Okazaki
            Aichi 444
            Japan
            Phone:  0564-55-7611
            Fax:    0564-53-7400
FEATURES             Location/Qualifiers
     CDS             161..2110
                     /EC_number="3.2.1.26"
                     /product="invertase"
                     /gene="invA"
                     /codon_start=1
                     /translation="MEHHKPLLPTSSHAAPTSSTRKDLLFVLCGLLFLSSLVAYGGYR
                     ASGVPHAHLSSPTSNHQQDHQSPTSLPSSKWYPVSRGVSSGVSEKSSNLLFAGEGGAS
                     EAFPWDNSMLSWQRTSFHFQPEKNWMNDPNGPMYYKGWYHFFYQYNPNGAVWGDIVWG
                     HAVSRDMIHWLHLPLAMVADQWYDKQGVWTGSATILPNGEIIMLYTGSTNESVQVQNL
                     AYPADPSDPLLLDWIKHTGNPVLVPPPGIGAKDFRDPTTAWLTSEGKWRITIGSKLNK
                     TGIALVYDTEDFKTYELKEGLLRAVPGTGMWECVDFFPVSKKNGNGLDTSVNGAEVKH
                     VMKVSLDDDRHDYYAIGTYDDNKVLFTPDDVKNDVGVGLRYDYGIFYASKTFYDQNKD
                     RRILWGWIGESDSEYADVTKGWASVQSIPRTVRLDTKTGSNLLQWPVDEVESLRLRSD
                     EFKSLKAKPGSVVSLDIETATQLDVVAEFEIDTESLEKTAESNEEFTCSSSGGAAQRG
                     ALGPFGLLVLADEGLSEYTPVYFYVIKGRNGNLRTSFCSDQSRSSQANDVRKQIFGSV
                     VPVLKGEKFSLRMLVDHSIVESFAQGGRTCVTSRVYPTKAIYGAARLFLFNNATEATV
                     TASLKVWQMNSAFIRPFPFNPDQKS"
     mat_peptide     464..2107
                     /product="invertase"
                     /codon_start=1
     polyA_site      2477
BASE COUNT      645 a    557 c    541 g    734 t
ORIGIN
        1 cttctgcaca cttccaaccc aaaccaatcc ccacacacat gcatgctgtc tctgccaact
       61 actcataaca caccataccc gattactcct tctctctttc tttcttttct ttctttcatt
      121 cacacccact tcctctctcc ctcatctttt ctttctcacc atggaacatc ataaaccctt
      181 gttacctact tcctctcacg ccgctccaac ctcatccact cgcaaggacc ttcttttcgt
      241 cctttgcggc ttgctcttcc tctcttccct cgtcgcctat gggggttaca gggcctccgg
      301 cgtgcctcat gctcaccttt catcacctac gtccaaccat caacaagatc accaaagtcc
      361 cacatcgcta ccttcttcca aatggtaccc tgtttcaagg ggtgtttctt ccggggtgtc
      421 cgagaagtca tcgaacttgt tattcgccgg tgaaggagga gcttccgaag catttccttg
      481 ggacaatagc atgttgtctt ggcagagaac ttcttttcat ttccagccag agaaaaactg
      541 gatgaacgat cctaatggtc ctatgtacta caagggatgg tatcactttt tctaccaata
      601 caatccgaac ggtgcagttt ggggtgacat agtttgggga cacgcggtgt caagggacat
      661 gatccattgg cttcaccttc cattggcaat ggtggctgat caatggtatg acaagcaagg
      721 cgtgtggaca ggctcagcta ccatcttacc aaatggtgaa atcatcatgt tatacacagg
      781 ttccaccaat gagtcagtgc aagttcaaaa cctggcatac cctgctgacc cctctgaccc
      841 tctgcttctg gattggatca aacacaccgg gaacccggtt ttggtcccac caccaggcat
      901 tggtgccaag gactttcgtg accctacaac tgcatggctc acctctgaag gaaagtggcg
      961 cataaccata ggatccaagc ttaataaaac tggcattgct ttggtttatg acactgagga
     1021 cttcaagacc tacgagctta aggaagggct gcttcgagct gtccctggca ctggcatgtg
     1081 ggagtgtgtc gacttcttcc ccgtgtccaa gaaaaatgga aatggattgg atacctctgt
     1141 taatggggct gaggtcaagc atgtcatgaa ggtcagcttg gatgatgata gacatgatta
     1201 ctacgccata gggacttacg atgataacaa agttctgttc acaccagatg atgttaagaa
     1261 tgatgttggt gttggtttaa ggtatgacta tggaatattc tatgcatcca agacatttta
     1321 tgatcagaac aaggatagga gaattttgtg gggttggatt ggagaatctg acagtgaata
     1381 tgcagatgtt accaaaggtt gggcttcagt tcagagtatt ccaagaactg tgaggcttga
     1441 tacgaagact ggcagcaact tacttcagtg gccggttgat gaggtggaga gtttgagatt
     1501 gagaagtgat gaattcaaaa gtttgaaggc aaaaccaggg tcagtggtgt cactagatat
     1561 tgaaacagcc acacagttgg acgttgttgc ggagtttgag atagacacgg aatccctaga
     1621 gaaaacagct gaatccaacg aggagttcac atgcagcagc agcggtggag ctgcacaacg
     1681 tggtgccttg ggaccttttg gtcttctggt tttggctgat gaggggcttt ctgagtatac
     1741 tcctgtgtat ttctacgtca ttaaaggaag aaatggaaat cttaggactt ccttttgctc
     1801 tgatcaatca aggtcttctc aggcaaatga cgttcgcaag caaatctttg ggagcgttgt
     1861 tccagtactt aaaggcgaaa agttttcctt acggatgctg gtggaccatt ctattgttga
     1921 aagctttgct caaggtggaa gaacgtgtgt aacatctcgg gtttatccaa caaaggcaat
     1981 ctatggagct gctagattgt tcttgttcaa caatgctact gaggccactg tgacagcctc
     2041 actcaaagtt tggcaaatga attctgcatt tatacgccca ttcccattca acccagatca
     2101 gaagagctaa agttcaaatt ggagatgaaa gatttatagt cacaaaggat tggaaaacct
     2161 cttgggttgg caatatgtgc ttatcaacat ctgtaatgac cttcccttaa acccagataa
     2221 agttatgggt tcttgttcaa tgccgattct ttcatttttg cccaattata ttcatcagat
     2281 gtttattgca atttgatctc ttatcatatg caattttagc gtacttgaga atatgttcct
     2341 caaagaaata cgatgttaat gttctccgat tctctgtaaa atcttttttt accttatttt
     2401 tttgagtttt gaggccaggt atagtaacaa ctatttattg tatattctta aaggtaatat
     2461 tatacttttg gtcattc
//