From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Trichoderma harzianum alkaline proteinase (Prb1) mRNA, complete
Date: 2 Sep 92 10:01:37 GMT
Distribution: bionet
Checksum: 65526     4

LOCUS       TRRBSCPRO    1458 bp ss-mRNA            PLN       02-SEP-1992
DEFINITION  Trichoderma harzianum alkaline proteinase (Prb1) mRNA, complete
            cds.
ACCESSION   M87516 M87517
KEYWORDS    alkaline proteinase; secreted protein.
SOURCE      Trichoderma harzianum cDNA to mRNA.
  ORGANISM  Trichoderma harzianum
            Eukaryota; Fungi; Eumycota; Ascomycotina; Pyrenomycetes;
            Hypocreales; Hypocreaceae.
REFERENCE   1  (bases 1 to 1458)
  AUTHORS   Geremia,R., Goldman,G., Jacobs,D., Vila,S., Ardiles,W.,
            Van Montagu,M. and Herrera-Estrella,A.
  TITLE     Purification and isolation of the gene of Prb1, an alkaline
            proteinase secreted by Trichoderma harzianum
  JOURNAL   Unpublished (1992)
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     sig_peptide     31..90
                     /note="The signal peptide for secretion and cleavage site
                     of the signal peptidase conform to the von Heijne rule."
                     /gene="Prb1"
                     /note="putative"
                     /codon_start=1
                     /citation=[1]
     CDS             31..1260
                     /note="prepropeptide"
                     /product="alkaline proteinase"
                     /gene="Prb1"
                     /codon_start=1
                     /translation="MTSIRRLALYLGALLPAVLAAPAALHKKPEAVPNKFIVTLKEGA
                     SIDTDSHLAWVTDIHTRSLTKRSTAGVEKTYNIHTWNAYAGEFDEETIEQIKSNPDVA
                     SVEPDYIMHLSDIVEDKRALTTQSGAPWGLGTVSHRTSGSTSYIYDSSAGAGTFAYVV
                     DSGINTSHQQFGGRASLGYNAAGGQHVDTLGHGTHVSGTIGGSTYGVAKQASLISVKV
                     FAGESASTSVILDGYNWAVNDIVSKSRASKSAINMSLGGPASSTWTTAINAAFNQGVL
                     TIVAAGNGDSLGNPQPVSGTSPANVPNAITVAALDINWRTASFTNYGAGVDVFAPGVN
                     ILSSWIGSNTATNTISGTSMATPHVVGLALYLQSLEGLTSPTAVTNRIKALATSGRVT
                     GSLNGSPNVIIFNGNSS"
     mat_peptide     391..1257
                     /product="alkaline proteinase"
                     /gene="Prb1"
                     /evidence=EXPERIMENTAL
                     /codon_start=1
                     /citation=[1]
BASE COUNT      337 a    407 c    332 g    382 t
ORIGIN
        1 ctcctccact atacaaagca catcacagcc atgaccagca ttcgtcgtct cgctctctat
       61 cttggagctt tgctcccggc tgtcctcgct gctcctgcag ctcttcacaa gaagcctgaa
      121 gccgtaccca acaagttcat cgtcactctt aaagagggtg cttctattga taccgactct
      181 catctcgcct gggtaacgga catccacacc cgttcgttga ccaagcgtag cactgctggt
      241 gttgaaaaga cttataacat tcatacttgg aatgcttatg cgggcgagtt tgacgaggag
      301 acaattgagc agatcaagtc caaccccgat gttgcgtctg ttgagccaga ctacatcatg
      361 catctgtcgg acattgttga agacaagcgt gctctgacca cacaatctgg tgctccttgg
      421 ggtctcggca ctgtgtccca tcgcacatct ggatccacaa gctacattta tgatagctca
      481 gccggcgccg gcacctttgc ctatgtggtt gactctggta tcaacacttc tcatcagcaa
      541 ttcggcgggc gtgccagcct tggctacaat gctgcaggag gacagcatgt cgatactctt
      601 ggccatggta ctcatgtttc cggaacaatt ggtggatcta catacggtgt tgctaagcag
      661 gcgagcctaa tctctgtcaa ggtctttgct ggtgaaagtg cttccacctc tgttatcctt
      721 gacggctata actgggctgt aaacgacatc gtctcgaaaa gccgtgctag caagtctgct
      781 attaacatgt cgcttggagg acctgcctca tctacctgga cgaccgccat taacgcagcc
      841 tttaaccagg gcgtgcttac cattgtcgcc gctggtaatg gcgactctct gggaaacccc
      901 cagccagtct ccggcacttc tcctgctaac gttcctaacg ccataaccgt tgccgcattg
      961 gacatcaact ggcgcactgc ttccttcacc aactatggtg ctggcgttga tgtttttgcc
     1021 cctggcgtca acattctgtc gtcatggatt ggatccaaca ctgctaccaa cacaatcagc
     1081 ggtacttcca tggctacacc tcacgttgtt ggcctggccc tctatcttca atctctcgag
     1141 ggtctcacct ctccgaccgc tgtcactaac cgaattaaag ctctggctac atctggtcgt
     1201 gtaaccggaa gcctcaacgg cagccccaac gttatcatct tcaacggaaa cagttcttaa
     1261 atcgttgatg gagcgaagga tgaagtgaaa tgttcatgga agggcagcac agctgttgct
     1321 tggcttgctt agctcattta agaagctaat caccatgatg gagtgcgatg ggtatataag
     1381 cttctacagc gttttcaact tagctagatg accctctata gatcgctaaa acaaaaagat
     1441 atgacagata cctgtgct
//