From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Aeromonas hydrophila DNA for prolyl endopeptidase, complete cds.
Date: 27 Jan 93 05:46:50 GMT
Distribution: bionet
Checksum: 37525 5

LOCUS       AEOPROEN     2230 bp ds-DNA             BCT       27-JAN-1993
DEFINITION  Aeromonas hydrophila DNA for prolyl endopeptidase, complete cds.
ACCESSION   D14005
KEYWORDS    prolyl endopeptidase.
SOURCE      Aeromonas hydrophila DNA.
  ORGANISM  Aeromonas hydrophila
            Prokaryotae; Bacteria; Gracilicutes; Scotobacteria; Facultatively
            anaerobic rods; Vibrionaceae.
REFERENCE   1  (bases 1 to 2230)
  AUTHORS   kanatani,A., Yoshimoto,T., Kokubo,T. and Tsuru,D.
  TITLE     Prolyl endopeptidase from Aeromonas hydrophila: Cloning, sequencing
            and expression of the enzyme gene, and characterization of the
            expressed enzyme
  JOURNAL   Unpublished (2048)
  STANDARD  full automatic
COMMENT     Submitted (05-JAN-1993) to DDBJ by: Tadashi Yoshimoto Nagasaki
            University School of Pharmaceutical Sciences 1-14 Bunkyou-machi
            Nagasaki 852 Japan Phone:  0958-47-1111 x2551 Fax:
            0958-44-6774.
FEATURES             Location/Qualifiers
     RBS             10..15
     CDS             20..2092
                     /EC_number="3.4.21.26"
                     /product="prolyl endopeptidase"
                     /codon_start=1
                     /translation="MSGKARLHYPVTRQSEQLDHYFGQAVADPYRWLEDDRSPETEAW
                     VKAQNRVTQDYLAQIPFRDAIKGKLATSWNYAKEGAPFREGRYHYFFKNDGLQNQNVL
                     CGQLAGKPAEVFLDPNLLSPDGTTALDQLSFSRDGKTLAYSLSLAGSDWREIHLMDVE
                     SKQPLETPLRDVKFSGISWLGNEGFFYSSYDKPDGSELSARTDQHKLYFHRLGTAQEE
                     DRLVFGAIPAQRHRYVGATVTEDDRYLLISAADSTSGNRLYVKDLTREGAPLLTVQGD
                     LAADVSLVDNKGSRLYLLTNRDAPNRRLVTVEADNPGPEQWRDLIPERQQVLTVHSGG
                     GYLFAEYMVDATARVEQFDHDGKRVREVGLPGLGSVSGFNGKQDDPALYFGFENYAQP
                     PTLYKFEPNSGAISLYRASAAPFKPEDYVSEQRFYRSKDGTRVPLIISYRKGLKLDGS
                     NPTILYGYGGFDVSLTPSFSVSVANWLDLGGVYAVANLRGGGEYGQAWHLAGTRMNKQ
                     NVFDDFIAAAEYLKAEGYTRTDRLAIRGGSNGGLLVGAVMTQRPDLMRVACQAVGVLD
                     MLRYHTFTAGAGWAYDYGTSADSEAMFDYLKGYSPLHSVRAGVSYPSTLVTTADHDDR
                     VVPAHSFKFAATLQADDAGPHPQLIRIETNAGHGAGTPVAKLIEQSADIYAFTLFEMG
                     YRQLPRQP"
BASE COUNT      405 a    738 c    725 g    362 t
ORIGIN      
        1 gtcaaccaaa gaggatgcca tgtcagggaa agcgcgcctt cactacccag ttacccgcca
       61 gagcgagcag ctggaccact acttcggcca ggccgtggcc gatccctacc gctggctgga
      121 ggatgatcgc agcccggaga ccgaggcctg ggtcaaggcc cagaaccggg tgacccagga
      181 ttacctggcg cagatcccgt ttcgcgatgc catcaagggc aagctggcca cctcctggaa
      241 ctacgccaag gagggggcgc cgtttcgcga ggggcgctac cactacttct tcaagaacga
      301 cggcctgcag aaccagaacg tgctgtgcgg ccagctggcg ggcaagccgg ccgaggtgtt
      361 cctcgatccc aacctgctca gccccgatgg caccacggcg ctggatcagc tgagcttctc
      421 ccgtgacggc aagacgcttg cctactcatt gtcgctggcc ggcagcgact ggcgcgagat
      481 ccacctgatg gacgtggaga gcaaacagcc gctggagaca cccctcaggg acgtgaagtt
      541 cagcggcatc agctggcttg gcaacgaggg tttcttctac tcgagctacg acaagccgga
      601 cggcagcgag ctgtcggcca ggacagatca gcacaagctc tacttccacc ggctcggcac
      661 ggcgcaggag gaggaccgcc tggtgttcgg cgccatcccg gcccagcggc accgctacgt
      721 gggggcgacc gtgactgaag atgatcgcta cctgctcatc tcggcggcgg actccacctc
      781 gggcaatcgc ctctatgtga aggacctgac ccgggagggg gccccgctgc tgacggtgca
      841 gggggatctg gctgcagacg tgagcctggt ggacaacaag ggcagccggc tttacctgct
      901 gaccaaccgg gatgccccca accgccggct ggtgactgtg gaggcggaca atccaggccc
      961 cgaacagtgg cgcgatctca tcccggagcg tcagcaggtg ctgacggtgc acagcggcgg
     1021 cggctacctg tttgccgagt acatggtgga cgccaccgcc agggtcgagc agttcgacca
     1081 tgacggcaag cgggtgcgcg aggtgggcct gccggggctc ggcagcgtga gcggcttcaa
     1141 cggcaagcag gatgatcccg ccctctactt cggcttcgag aactatgccc agccgcccac
     1201 cctctacaag ttcgagccca acagcggggc catcagcctc tatcgcgcct cggctgcccc
     1261 gttcaaaccg gaggattatg tctccgagca gcgtttctac cggagcaaag acggcacccg
     1321 ggtgccgctc atcatcagct accgcaaggg gctaaaactc gatggcagca acccgaccat
     1381 cctctacggc tatggcggct tcgacgtgag cctgaccccg agcttcagcg tctcggttgc
     1441 caactggctg gatctggggg gcgtttatgc ggtggccaac ctgcgtggcg gtggcgagta
     1501 cggccaggcc tggcatttgg cgggcaccag gatgaacaag cagaacgtgt tcgacgactt
     1561 catcgccgca gccgagtacc tcaaggccga gggttacacc cgcaccgacc ggctggccat
     1621 cagaggcggc tccaacggcg gtctgctggt gggggccgtc atgacccagc ggccggatct
     1681 gatgcgggtc gcctgccagg ctgtgggggt gctcgacatg ctgcgctacc acaccttcac
     1741 cgccggggcg ggctgggcct atgactacgg caccagcgcc gacagcgagg ccatgttcga
     1801 ctatctgaag ggctactcgc cgctgcacag cgtccgggcc ggggtcagct acccctcgac
     1861 cctggtgacc acggcggatc acgacgaccg tgtggtgccg gctcactcct tcaagtttgc
     1921 cgccaccctg caggctgacg acgcaggccc ccatccccag ctcatccgca tcgagaccaa
     1981 tgccgggcac ggggcgggca cgccggtggc caagctgatc gagcagagtg cggacatcta
     2041 tgccttcacc ttgttcgaga tgggttaccg gcaactgccg cgccaaccat gacccaggcg
     2101 atatgggtta ccggcagttg ccggtcagcc cctgagatca gggccctgcg gggccctttt
     2161 tgttgtcagc cccggctgcg accatccctg tctcgccgcg atccggtttc gggcggcggg
     2221 ggcatccgcg
//