From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Clostridium perfringens beta-galactosidase alpha-peptide (lacZ)
Date: 31 Dec 92 01:34:08 GMT
Distribution: bionet
Checksum: 30474 13

LOCUS       CLOCLSB      5954 bp ds-DNA             BCT       17-DEC-1992
DEFINITION  Clostridium perfringens beta-galactosidase alpha-peptide (lacZ)
            gene, partial cds; replication enzyme (rep) gene, 3' end cds; RNA
            methylase (ermBP) gene, complete cds.
ACCESSION   L02938
KEYWORDS    RNA methylase; beta-galactosidase alpha-peptide; ermBP gene;
            lacZ gene; multiple cloning site; rep gene; replication enzyme;
            replication origin; replication region.
SOURCE      Clostridium perfringens DNA.
  ORGANISM  Clostridium perfringens
            Prokaryotae; Firmicutes; Endospore-forming rods and cocci;
            Bacillaceae.
REFERENCE   1  (sites)
  AUTHORS   Garnier,T. and Cole,S.T.
  TITLE     Characterization of a bacteriocinogenic plasmid from Clostridium
            perfringens and molecular genetic analysis of the
            bacteriocin-encoding gene
  JOURNAL   J. Bacteriol. 168, 1189-1196 (1986)
  STANDARD  full automatic
REFERENCE   2  (sites)
  AUTHORS   Berryman,D.I. and Rood,J.I.
  JOURNAL   Unpublished (1991)
  STANDARD  full automatic
REFERENCE   3  (bases 1 to 5954)
  AUTHORS   Bannam,T.L. and Rood,J.I.
  TITLE     Clostridium perfringens - Escherichia coli shuttle vectors which
            carry single antibiotic resistance determinants
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
REFERENCE   4  (bases 1 to 5954)
  AUTHORS   Sloan,J., Warner,T.A., Scott,P.T., Bannam,T.L., Berryman,D.I. and
            Rood,J.I.
  TITLE     Construction of a sequenced Clostridium perfringens-Escherichia
            coli shuttle plasmid
  JOURNAL   Antimicrob. Agents Chemother. 27, 207-219 (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     CDS             <310..>5941
                     /product="beta-galactosidase alpha-peptide"
                     /gene="lacZ"
                     /codon_start=1
                     /partial
                     /note="derived from pUC18 (GenBank accession #VB0025;
                     putative"
     misc_feature    1..57
                     /standard_name="multiple cloning site (MCS)"
                     /evidence="EXPERIMENTAL"
                     /note="derived from pUC18 (GenBank accession #VB0025)"
     misc_feature    1190..1419
                     /function="C. perfringens pIP404 replication origin"
                     /standard_name="ori (oriCP)"
                     /evidence="EXPERIMENTAL"
                     /citation=[1]
                     /note="derived from complete sequence of pIP404 (Garnier
                     and Cole, Plasmid 19, 134-150. 1988; GenBank accession
                     #M14481)"
     misc_feature    5376..5596
                     /function="replication region from pUC18"
                     /standard_name="oriEC"
                     /note="putative"
     CDS             complement(<1482..2702)
                     /function="pIP404 replication"
                     /gene="rep"
                     /citation=[1]
                     /note="derived from complete sequence of pIP404 (Garnier
                     and Cole, Plasmid 19, 134-150. 1988; GenBank accession
                     #M14481); putative"
                     /product="replication enzyme"
                     /codon_start=1
                     /translation="VRNALKLNKKNYIDNIHSRSKGWITRSVIDKKGYSQWHYKYAEL
                     KDLDMSDENIYITLNTFYKPCRRLENIKELNTLFIDLDYYKTGKTKDQVLMDLEKNYF
                     NQSIPIPNYVIDSGRGMYLIWIINAVPSKALPLWKAVQEYLYNQLKYFGADRQALDAT
                     RILRVPGSINSKSKTVVNILDEYEYIYDLREIQNGFLPELKPYERKKGRPSKINYIYR
                     ERSLYYGRIQDIIKLCELREYDLKGHRELILFLYRYYLCSFTEDIEKALNDVLELNSM
                     FRQHLSEREVIRATRSAERCYLDKNKQYKYKNETLIELLEITEEEQKYMTIIISKKEY
                     KRRENIRGKKNYQEQLKAKGKATKKEELNVLRKKIKALKEKGFKNKEITLMLEVPIKT
                     LERHITYMKKNGLL"
     CDS             complement(3761..4498)
                     /function="methylation of 23S rRNA"
                     /gene="ermBP"
                     /evidence="EXPERIMENTAL"
                     /citation=[2]
                     /note="derived from sequence of C. perfringens ermBP gene
                     region (D. Berryman and J. Rood, unpublished results; EMBL
                     accession #X58285)"
                     /product="RNA methylase"
                     /codon_start=1
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT     1836 a   1114 c    918 g   2086 t
ORIGIN      
        1 gaattcgagc tcggtacccg gggatcctct agagtcgacc tgcaggcatg caagcttggc
       61 actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg
      121 ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg
      181 cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc ctgatgcggt attttctcct
      241 tacgcatctg tgcggtattt cacaccgcat atggtgcact ctcagtacaa tctgctctga
      301 tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc
      361 ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg
      421 tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg tgatacgcct
      481 atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg
      541 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
      601 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
      661 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
      721 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
      781 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
      841 acgttttcca atgatgagca cttttaaatt aaaaatgaag ttttaaaact tcatttttaa
      901 tttaaattaa aaatgaagtt ttatcaaaaa aatttccaat aatcccactc taagccacaa
      961 acacgcccta taaaatcccg ctttaatccc actttgagac acatgtaata ttactttacg
     1021 ccctagtata gtgataattt tttacattca atgccacgca aaaaaataaa ggggcactat
     1081 aataaaagtt ccttcggaac taactaaagt aaaaaattat ctttacaacc tccccaaaaa
     1141 aaagaacagg tacaaagtac cctataatac aagcgtaaaa aaatgagggt aaaaataaaa
     1201 aaataaaaaa ataaaaaaat aaaaaaataa aaaaaataaa aaaataaaaa aataaaaaaa
     1261 taaaaaaata aaaaaataaa aaaataaaaa aataaaaaaa tataaaaata aaaaaatata
     1321 aaaataaaaa aatataaaaa taaaaaaata taaaaataaa aaaataaaaa aatataaaaa
     1381 taaaaaaata aaaaaatata aaaatatttt ttatttaaag tttgaaaaaa atttttttat
     1441 attatataat ctttgaagaa aagaatataa aaaatgagcc tttataaaag cccatttttt
     1501 ttcatatacg taatatgacg ttctaatgtt tttattggta cttctaacat tagagtaatt
     1561 tctttatttt taaagccttt ttctttaagg gcttttattt tttttcttaa tacatttaat
     1621 tcctcttttt ttgttgcttt tcctttagct tttaattgct cttgataatt ttttttacct
     1681 ctaatatttt ctcttctctt atattccttt ttagaaatta ttattgtcat atatttttgt
     1741 tcttcttctg taatttctaa taactctata agagtttcat tcttatactt atattgctta
     1801 tttttatcta aataacatct ttcagcactt ctagttgctc ttataacttc tctttcactt
     1861 aaatgttgtc taaacatact attaagttct aaaacatcat ttaatgcctt ctcaatgtct
     1921 tctgtaaagc tacaaagata atatctatat aaaaataata taagctctct gtgtcctttt
     1981 aaatcatatt ctcttagttc acaaagtttt attatgtctt gtattcttcc ataatataaa
     2041 cttctttctc tataaatata atttattttg cttggtctac cctttttcct ttcatatggt
     2101 tttaattcag gtaaaaatcc attttgtatt tctcttaagt cataaatata ttcgtactca
     2161 tctaatatat tgactactgt ttttgattta gagtttatac ttcctggaac tcttaatatt
     2221 ctggttgcat ctaaggcttg tctatctgct ccaaagtatt ttaattgatt atataaatat
     2281 tcttgaaccg ctttccataa tggtaatgct ttactaggta ctgcatttat tatccatatt
     2341 aaatacattc ctcttccact atctattaca tagtttggta taggaatact ttgattaaaa
     2401 taattctttt ctaagtccat taatacctgg tctttagttt tgccagtttt ataataatcc
     2461 aagtctataa acagtgtatt taactctttt atattttcta atcgcctaca cggcttataa
     2521 aaggtattta gagttatata gatattttca tcactcatat ctaaatcttt taattcagcg
     2581 tatttatagt gccattggct atatcctttt ttatctataa cgctcctggt tatccaccct
     2641 ttacttctac tatgaatatt atctatatag ttctttttat tcagctttaa tgcgtttctc
     2701 acttattcac ctccccttct gtaaaactaa gaaaattata tcatattttc aataattatt
     2761 aactattctt aaactcttaa taaaaaatag agtaagtccc caattgaaac ttaatctatt
     2821 ttttatgttt taatttatta tttttattaa aatattttaa actaaattaa atgattcttt
     2881 ttaatttttt actatttcat tccataatat attactataa ttatttacaa ataatatttc
     2941 ttcatttgta atatttagat gatttactaa ttttagtttt tatatattaa ataattaatg
     3001 tataatttat ataaaaaatc aaaggagctt ataaattatg attatttcca aagatactaa
     3061 agatttaatt ttttcaattt taacaatact ttttgtaata ttatgtttaa atttaattgt
     3121 atttttttca tataataaag ccgttgaagt aaaccaatcc attttcctta tgatgttatt
     3181 attaaattta agttttataa taatatcttt attatattta ttgtttttaa aaaaactagt
     3241 gaaatttccg gctttattaa acttattttt aggaatttta ttttcatttt catctttaca
     3301 ggatttgatt atatctttaa atatgtttta tcaaatatta tctttttcta aatttatata
     3361 tatttttatt atatttatta ttatatatat tttattttta agtttctttc taacagctat
     3421 taaaaagaaa cttaaaaata aaaacacgta ctctaaacca ataaataaaa ctatttttat
     3481 tattgctgcc ttgattggaa tagtttttag taaaattaat ttcaatattc cacaatatta
     3541 tattataagc tagctttgca ttgtactttt caatcgcttc acgaatgcgg ttatctccga
     3601 aagataaagt cttttcatct tccttgatga agataagatt ttctccgtct ccgccgacct
     3661 ctttagctcc ttggaagctg tcagtagtat acctaataat ttatctacat tccctttagt
     3721 aacgtgtaac tttccaaatt tacaaaagcg actcatagaa ttatttcctc ccgttaaata
     3781 atagataact attaaaaata gacaatactt gctcataagt aacggtactt aaattgttta
     3841 ctttggcgtg tttcattgct tgatgaaact gatttttagt aaacagttga cgatattctc
     3901 gattgaccca ttttgaaaca aagtacgtat atagcttcca atatttatct ggaacatctg
     3961 tggtatggcg ggtaagtttt attaagacac tgtttacttt tggtttagga tgaaagcatt
     4021 ccgctggcag cttaagcaat tgctgaatcg agacttgagt gtgcaagagc aaccctagtg
     4081 ttcggtgaat atccaaggta cgcttgtaga atccttcttc aacaatcaga tagatgtcag
     4141 acgcatggct ttcaaaaacc acttttttaa taatttgtgt gcttaaatgg taaggaatac
     4201 tcccaacaat tttatacctc tgtttgttag ggaattgaaa ctgtagaata tcttggtgaa
     4261 ttaaagtgac acgagtattc agttttaatt tttctgacga taagttgaat agatgactgt
     4321 ctaattcaat agacgttacc tgtttactta ttttagccag tttcgtcgtt aaatgccctt
     4381 tacctgttcc aatttcgtaa acggtatcgg tttcttttaa attcaattgt tttattattt
     4441 ggttgagtac tttttcactc gttaaaaagt tttgagaata ttttatattt ttgttcatgt
     4501 aatcactcct tcttaattac aaatttttag catctaattt aacttcaatt cctattatac
     4561 aaaattttaa gatactgcac tatcaacaca ctcttaagtt tgcttctaag tcttatttcc
     4621 ataacttctt ttacgtttcc gggtacaatt cgtaatcatg tcatagctgt ttcctgtgtg
     4681 aaattcttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     4741 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     4801 ccagtcggga aacctgtcgt gccagaaaac ttcattttta atttaaaagg atctaggtga
     4861 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     4921 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     4981 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     5041 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     5101 tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     5161 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     5221 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     5281 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     5341 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     5401 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     5461 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     5521 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     5581 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     5641 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     5701 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
     5761 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
     5821 gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc
     5881 ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct
     5941 atgaccatga ttac
//