From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: D.pseudoobscura Adh gene for alcohol dehydrogenase
Date: 28 Dec 92 21:26:36 GMT
Distribution: bionet
Checksum: 44745 8

LOCUS       DROADHGN17   3490 bp ds-DNA             INV       01-DEC-1992
DEFINITION  D.pseudoobscura Adh gene for alcohol dehydrogenase
ACCESSION   X62197
KEYWORDS    ADH gene; alcohol dehydrogenase.
SOURCE      Drosophila pseudoobscura DNA.
  ORGANISM  Drosophila pseudoobscura
            Eukaryota; Animalia; Metazoa; Arthropoda; Uniramia; Insecta;
            Pterygota; Neoptera; Holometabola; Diptera; Brachycera;
            Cyclorrhapha; Schizophora; Drosophiloidea; Drosophilidae.
REFERENCE   1  (bases 1 to 3490)
  AUTHORS   Schaeffer,S.W. and Miller,E.L.
  TITLE     Estimates of gene flow in Drosophila pseudoobscura determined from
            nucleotide sequence analysis of the alcohol dehydrogenase region
  JOURNAL   Genetics 132, 471-480 (1992)
  STANDARD  full automatic
COMMENT     *source: strain=GB118; *source: isolate=Gundlach Bundschu Winery,
            Sonoma Valley, *source: California; *source: chromosome=IV section
            88;
               From EMBL    entry DPADHGN17;  dated 16-OCT-1992.
FEATURES             Location/Qualifiers
     mRNA            join(40..119,923..1028,1109..1513,1574..1989)
                     /note="adult mRNA (Adh)"
     mRNA            join(890..1028,1109..1513,1574..1989)
                     /note="larval mRNA (Adh)"
     promoter        8..14
                     /note="adult promoter (Adh)"
     prim_transcript 40..1989
                     /note="adult primary transcript (Adh)"
     exon            40..119
                     /note="adult exon 1a"
     intron          120..922
                     /note="adult intron"
     promoter        854..861
                     /note="larval promoter (Adh)"
     prim_transcript 890..1989
                     /note="larval primary transcript (Adh)"
     exon            890..1028
                     /note="larval exon 1"
     exon            923..1028
                     /note="adult exon 1b"
     intron          1029..1108
                     /number=1
     exon            1109..1513
                     /number=2
     intron          1514..1573
                     /number=2
     exon            1574..1989
                     /number=3
     polyA_signal    1966..1971
     polyA_site      1989
     CDS             join(936..1028,1109..1513,1574..1840)
                     /EC_number="1.1.1.1"
                     /gene="Adh"
                     /product="alcoholdehydrogenase"
                     /codon_start=1
                     /translation="MSLTNKNVVFVAGLGGIGLDTSRELVKRNLKNLVILDRIDNPAA
                     IAELKAINPKVTITFYPYDVTVPVAETTKLLKTIFAQVKTIDVLINGAGILDDHQIER
                     TIAVNYTGLVNTTTAILDFWDKRKGGPGGIICNIGSVTGFNAIYQVPVYSGSKAAVVN
                     FTSSLAKLAPITGVTAYTVNPGITKTTLVHKFNSWLDVEPRVAEKLLEHPTQTSQQCA
                     ENFVKAIELNKNGAIWKLDLGTLEPITWTQHWDSGI"
BASE COUNT      967 a    803 c    799 g    921 t
ORIGIN      
        1 cgtagtgtat ttaaggcact gcacatgtcg aggaccacga ttattgtcta cagtgcagtc
       61 agtagttgtc aggtgcagca ggcggaccag catttcttta aatctactca atcgatcaag
      121 taagtaactg taatccaagt acagtcggcc catcccactc ggtcactcgg ttctgccgta
      181 attcaagaaa tttcttgata gcgcagttcc agttgtaaaa cgtcgtttaa aaaaacatct
      241 cgttggagct tgggttggaa aacgttcgag ttgggcgtag aaaatttcag atataaaata
      301 tcaaaaaaga gaaaatatct tcccaaagca gctgtccatt ttttgttaaa gtgatagatc
      361 aaaaattcgt atgacgtatt tcccaagtgc tcttcatgca aacaatttcc tcaattctaa
      421 ttctacaaga agcacttttg gctcttgtcc agaaaaaaca atttgtttac acttttcggt
      481 tgataacaga ggcggaccga cggctaagta acataacagt cgcctgttaa gtaatgccct
      541 cactctctgt tagatggccg cgcgtgtcga ctccgataga ccagcagaca cgcagtagtt
      601 ttcctcaaga acctgaactc taaacataga cataatttac gataccttga gactcaaata
      661 aaaatacaag tatgagtggt attctattcc tgattagact cgcacacaca ctcgtacgag
      721 tattaacata aatttgccgg caaataacca aaacaaaacg aacgcaaacg atccgaacac
      781 gtaatgcgag agataagaaa cagaaaagct cgacgtgaaa gaagcttctg gcggaagctc
      841 ttgccgaact ccctataaat acgagacaga aaaccagaat aaatataata acaaactgtt
      901 gaattgtccc atcgattccc agatcaaaag acagaatgtc tctcaccaac aagaacgtcg
      961 ttttcgtggc cggtctgggc ggcattggcc tggacaccag ccgggagttg gtcaagcgta
     1021 atctgaaggt aagagagatg gagagagaga gcaatccctt ttccatggaa tctatggaat
     1081 cctaaattta aaaattcatt tattttagaa cctggtcatc ctggatcgca ttgacaatcc
     1141 ggctgccatt gccgaactga aggcaatcaa tcccaaggtg accatcacct tctatcccta
     1201 cgatgtgact gtgcccgtcg ctgagaccac caagctcctg aagaccatct ttgcccaggt
     1261 gaagacaatc gatgtcctga tcaacggtgc tggcatcctg gacgatcatc agatcgagcg
     1321 caccattgcc gttaactaca cgggcctggt caacaccacc acagccattc tggacttctg
     1381 ggacaagcgc aagggcggcc caggcggcat catttgcaac attggctccg tcaccggttt
     1441 caatgccatc taccaggtgc ccgtttactc cggctccaag gcggcggtgg ttaactttac
     1501 ctcctccctg gcggtaagta atccaacgtt tttcctactc cccaaaacta aatatttctt
     1561 tgaaatccca tagaaactgg ctcccattac tggtgtcact gcttacactg tcaatcctgg
     1621 catcactaag accactctgg tccacaaatt caactcgtgg ctggatgtgg agccccgtgt
     1681 ggcggagaag ctgctcgagc atcccaccca gacctctcag cagtgcgccg agaactttgt
     1741 gaaggccatt gagctgaaca agaacggtgc catctggaag ttggatctgg gcaccttgga
     1801 gcccatcaca tggacccagc actgggactc gggcatctaa accatcccag agactctatg
     1861 ggacatggcg ttttagcttt tagtttcttt ttttccactc aattgttacg tatatacata
     1921 cacatatggc aataaggctg atttgaaccc cgttttgaat atgataatac aaaattatat
     1981 ttgagaaatt tcaacaaaat cgataagagc tatagctctc ttttaattaa aaaaaactag
     2041 ataaaaagag caatggtcag tggtggtcag tggtcgttgt ggcctctatc gatttccaca
     2101 caaaaacttt actgtttagt aatagaaaag aacttgagaa aggcagccaa aagaatgtac
     2161 gatctgacgg gtaagcatgt ctgctatgtg gctgactgcg gtggcattgc actggagacc
     2221 agcaaggttc tcatgaccaa gaatatagcg gtgagtggag agtggagagt gttccaaagt
     2281 ggttctggtt cttaaacaag tctctagtct ctagtctcta gtctcttggc tgctgctggt
     2341 agctgtcgaa actaattaag ttaatgactt tttcatgttt tgtttttgtc cgctgacttt
     2401 tgctgttcaa agctacaatt aagtttatgt tttgagtttg atctactttt gcttaaggga
     2461 agtactttcg attacggatt gctggcatat aaaaatagat accaaggtat gtgcatcata
     2521 ttgattctat cttgattctt tgcccagaaa ctggccatcc ttcagagcgt ggaaaacccc
     2581 ccggccatcg ctcagttaca atcgattaag cacagcacac agatcttctt ctggaccttc
     2641 gatgtgacca tggcccgcga ggagatgaag aagtacttcg atgaggtcat ggtccagatg
     2701 gactacatcg atgtactgat caatggggcc accctgtgcg atgagcggaa cattgatgcc
     2761 accatcaata cgaatctgac cggcatgatg aacaccgtgg ccactgtgct gccctatatg
     2821 gaccgtaaga tgggcggttc gggtggactg attgtgaatg tcacctccgt cataggactg
     2881 gatccatcgc cggtcttttg tgcgtacagt gcctcaaagt ttggtgtgat tgggttcacc
     2941 cgaagtctag cggtgagtgg gcgagatctt aacttaaaaa tgctctctaa tgtcttccat
     3001 tctttcgcag gatcccctgt attacaccca aaatggtgtg gctgtcatgg ccgtctgctg
     3061 tggtcccacc aaagtgtttg tcgatcgcga actgactgcc ttcctggaat acggccagtc
     3121 ctttgccgat cgcctgcgtc gtgccccctg ccaatcgacg gccgtctgcg gccagaatat
     3181 agttaatgcc atcgagagat cggagaacgg acagatctgg attgccgaca agggcggcct
     3241 ggagtcggtg gccctccact ggtattggca tatggccgat cagtttgtga actacatgca
     3301 gagcactgac gatgaggatc aggagctttt tttggggcag cgatgaggca tgccttgatc
     3361 cacggaataa aacatcaaat gtgtcacaaa aagtaggcaa cttgtttgtg ggtctggaaa
     3421 tagatttgaa ttattttgag gtcatatgaa taatgacata aatgagggtg cctgaaagta
     3481 tgccaagttt
//