From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Pseudomonas lipase and its activator genes, complete cds.
Date: 9 Apr 92 10:04:53 GMT
Distribution: bionet
Checksum: 17743     7

LOCUS       PSELIPACT    2916 bp ds-DNA             BCT       09-APR-1992
DEFINITION  Pseudomonas lipase and its activator genes, complete cds.
ACCESSION   D10069 D01216
KEYWORDS    lipase; lipase-activator.
SOURCE      Pseudomonas sp. (strain KWI-56) DNA.
  ORGANISM  Pseudomonas sp.
            Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
            Pseudomonadaceae.
REFERENCE   1  (bases 1 to 2916)
  AUTHORS   Iizumi,T., Nakamura,K., Shimada,Y., Sugihara,A., Tominaga,Y. and
            Fukase,T.
  TITLE     Cloning, nucleotide sequencing, and expression in Escherichia coli
            of a lipase and its activator genes from Pseudomonas sp.KWI-56
  JOURNAL   Agric. Biol. Chem. 55, 2349-2357 (1991)
  STANDARD  full staff_review
COMMENT     Data kindly submitted in computer readable form by:
            Taro Iizumi
            Kurita Water Industries LTD.
            7-1 Wakamiya, Morinosato
            Atsugi, Kanagawa 243-01
            Japan
            Phone:  0462-48-2575
            Fax:    0462-48-2545
FEATURES             Location/Qualifiers
     RBS             366..370
     CDS             378..1472
                     /EC_number="3.1.1.3"
                     /product="lipase"
                     /gene="lip"
                     /codon_start=1
                     /translation="MARTMRSRVVAGAVACAMSIAPFAGTTAVMTLATTHAAMAATAP
                     ADGYAATRYPIILVHGLSGTDKYAGVVEYWYGIQEDLQQNGATVYVANLSGFQSDDGA
                     NGRGEQLLAYVKTVLAATGATKVNLVGHSQGGLTSRYVAAVAPDLVASVTTIGTPHRG
                     SEFADFVQNVLAYDPTGLSSSVIAAFVNVFGILTSSSHNTNQDALAALQTLTTARAAT
                     YNQNYPSAGLGAPGSCQTGAPTETVGGNTHLLYSWAGTAIQPTLSVFGITGATDTSTV
                     PLVDLANVLDPSTLALFGTGTVMINRGSGQNDGLVSKCSALYGKVLSTSYKWNHLDEI
                     NQLLGVRGAYAEDPVAVIRTHANRLKLAGV"
     CDS             1476..2510
                     /product="lipase-activator"
                     /gene="act"
                     /codon_start=1
                     /translation="MTSREGRAPLARRAVVYGVVGLAAIAGVAMWSGAGWHRATGASG
                     ESPEASVAGGSVTAPPQAAVPASTGLPPSLAGSSAPRLPLDAGGHLAKSRAVRDFFDY
                     CLTAQSDLSAAGLDAFVMREIAAQLDGTVAQAEALDVWHRYRAYLDALAKLRDAGAAD
                     KSDLGALQLALDQRASIAYRTLGDWSQPFFGAEQWRQRYDLARLKIAQDPTLTDAQKA
                     ERLAALEQQMPADERAAQQHIDQQRAAIDQIAQLQKSGATPDAMRAQLTQTLGPEAAA
                     RVAQMQQDDASWQSRYADYAAQRTQIESAGLSPQDRDAQIAALRQRVFTRPGEAVRAA
                     SLDRGAGSAR"
BASE COUNT      491 a    960 c   1032 g    433 t
ORIGIN
        1 agatctcggt cggcgtggac aacgtgctga acaaggccta caccgagcac ctgaacctcg
       61 ccggcaacgc gggcttcggc tatccggcga actcgccggt gatggagccg ggccggaccg
      121 catgggtgcg cgtgagtgtg aagctgtaac gcatcgcgcg tgtgcgttgc agcatgcgca
      181 cgcgtgaacg cagccccgcc tgaacaggcg gggcgcgtca accgattaga gaaccgtgtc
      241 tagtcagggc gcaaacgttc gcgactcgtg attcactccc gcattcgacg cacacgatgc
      301 tcgcgacgtt cgcgacgcat agtgtgcgtc gatccggctt cattcgcacc ggcagcacaa
      361 taatcaggag aacatgcatg gccagaacga tgcgttccag ggtggtggca ggagcagtgg
      421 catgcgcgat gagcatcgcg ccgttcgcgg ggacgaccgc agtgatgacg ctcgcgacga
      481 cgcacgcggc gatggcggcg accgcgcccg ccgatggcta cgcggcgacg cgttatccga
      541 tcatcctcgt gcacgggctc tcgggtaccg acaagtacgc cggcgtggtc gagtattggt
      601 atggcatcca ggaagacctg cagcagaacg gtgcgaccgt ctacgtcgcg aacctgtcgg
      661 ggttccagag cgacgacggc gcgaacgggc gcggcgaaca gttgctcgct tacgtgaaga
      721 cggtgctcgc ggcgacgggc gcgaccaagg tcaatctcgt cggccacagc cagggcggcc
      781 tcacgtcgcg ctatgtcgcg gccgtcgcgc ccgatctcgt ggcgtcggtg acgacgatcg
      841 gcacgccgca tcgcggctcc gagtttgccg acttcgtgca gaacgtgctg gcgtacgatc
      901 cgaccgggct ttcgtcatcg gtgatcgccg cgttcgtcaa tgtgttcggc atcctgacga
      961 gcagcagcca caacacgaac caggacgcgc tcgccgcgct gcagacgctg accaccgccc
     1021 gggctgccac gtacaaccag aactatccga gcgcgggcct gggtgcgccg ggcagttgcc
     1081 agaccggcgc gccgaccgaa accgtcggcg gcaacacgca cctgctgtat tcgtgggccg
     1141 gcacggcgat ccagccgacg ctttcggtgt tcggcatcac gggcgcgacc gacacgagca
     1201 ccgttccgct cgttgatctg gcgaacgtgc tcgacccgtc gacgctcgcg ctgttcggca
     1261 ccggcacggt gatgatcaac cgcggctccg ggcagaacga cgggctcgtg tcgaagtgca
     1321 gtgcgctgta cggcaaggtg ctgagtacga gctacaagtg gaaccacctc gacgagatca
     1381 accagctgct cggcgtgcgc ggcgcgtatg cggaagatcc ggtcgcggtg atccgcacgc
     1441 atgcgaaccg gctgaagctg gcgggcgtgt aatcgatgac gtcacgtgaa ggacgcgcgc
     1501 cgctggcgcg gcgcgccgtg gtctatggtg tcgtggggct ggcggcgatt gccggcgtgg
     1561 cgatgtggag cggcgcgggc tggcatcgcg caacgggcgc ttccggcgag tcgccggagg
     1621 cgtcggtggc agggggatcg gttaccgcac cgccgcaggc agccgtgccg gcaagcacgg
     1681 gcttgccgcc gtcactcgcc ggctccagcg cgccgcggtt gccgctcgat gccggcgggc
     1741 atctcgcgaa gtcgcgcgca gtgcgggatt tcttcgacta ctgcctcacc gcgcagagcg
     1801 acctgagcgc ggccggtctc gacgcgttcg tcatgcgcga gattgccgca cagctcgacg
     1861 gtaccgttgc gcaagccgag gcgctcgacg tgtggcaccg gtatcgcgcg tatctcgacg
     1921 cactcgcgaa attgcgcgat gccggcgcgg ccgacaagtc cgacctgggc gcgttgcaac
     1981 tcgcgctcga ccagcgcgcg tcgatcgcgt accgcacgct cggcgactgg agccagccgt
     2041 tcttcggtgc ggagcagtgg cggcagcgct acgacctggc gcgactgaag atcgcgcagg
     2101 atcccacgct gacggatgcg cagaaggccg aacggctcgc ggcgctcgaa cagcagatgc
     2161 cggccgacga acgcgccgcg cagcagcaca tcgaccagca gcgtgcggcg atcgaccaga
     2221 tcgcgcaatt gcagaagagc ggggcgacac ccgatgcgat gcgcgcacaa ctgacgcaga
     2281 cgctcggccc cgaagcggcc gcgcgcgtcg cgcagatgca gcaggacgac gcatcgtggc
     2341 agagccgcta cgcggactat gcggcgcagc gcacgcagat cgaatcggcc ggcctgtcgc
     2401 cgcaggatcg cgacgcgcag atcgccgcgc tgcggcagcg cgtgttcacg cggcccggcg
     2461 aagccgtgcg tgcggcatcg ctcgatcgcg gggcgggcag cgcgcggtaa cgcgggcggc
     2521 gcagcgcatg accgtcgcta tgccgcgcgc gtgagatccc gcggcaggtg ttgctcgatg
     2581 gtattgcgca gcgtgtcgaa cgccggctcg atgccttcgt gcgcgacgca tgcataaccg
     2641 agcagcaggc cgcgccgcgc ggtgtcgagg ctgctgtagt agctggtgag cgggcgcacg
     2701 atcacgcccg catcgaacgc gctctgcgtg accgcgcgat cgtcgcaggc gtcgggcagg
     2761 ccgagcacga gatgcaggcc tgcctcgtcc cccatcaccg gcagcgcgtc gccgaagcgc
     2821 gcgcggatcg cgtcgatcat cagttgccgg cgctcgccgt acagcgtgcg catccgtctg
     2881 acgtgcgaag tcaggtggcc gtccatgaat gaattc
//