From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: D.pseudoobscura Adh gene for alcohol dehydrogenase
Date: 28 Dec 92 21:28:05 GMT
Distribution: bionet
Checksum: 57945 8

LOCUS       DROADHGN76   3472 bp ds-DNA             INV       01-DEC-1992
DEFINITION  D.pseudoobscura Adh gene for alcohol dehydrogenase
ACCESSION   X64485
KEYWORDS    ADH gene; alcohol dehydrogenase.
SOURCE      Drosophila pseudoobscura DNA.
  ORGANISM  Drosophila pseudoobscura
            Eukaryota; Animalia; Metazoa; Arthropoda; Uniramia; Insecta;
            Pterygota; Neoptera; Holometabola; Diptera; Brachycera;
            Cyclorrhapha; Schizophora; Drosophiloidea; Drosophilidae.
REFERENCE   1  (bases 1 to 3472)
  AUTHORS   Schaeffer,S.W. and Miller,E.L.
  TITLE     Estimates of gene flow in Drosophila pseudoobscura determined from
            nucleotide sequence analysis of the alcohol dehydrogenase region
  JOURNAL   Genetics 132, 471-480 (1992)
  STANDARD  full automatic
COMMENT     *source: strain=PS282; *source: isolate=Kiabab National Forest,
            Arizona; *source: chromosome=IV section 88;
               From EMBL    entry DPADHGN76;  dated 16-OCT-1992.
FEATURES             Location/Qualifiers
     mRNA            join(40..119,914..1019,1083..1487,1548..1963)
                     /note="adult mRNA (Adh)"
     mRNA            join(881..1019,1083..1487,1548..1963)
                     /note="larval mRNA (Adh)"
     promoter        8..14
                     /note="adult promoter (Adh)"
     prim_transcript 40..1963
                     /note="adult primary transcript (Adh)"
     exon            40..119
                     /note="adult exon 1a"
     intron          120..913
                     /note="adult intron"
     prim_transcript 881..1963
                     /note="larval primary transcript (Adh)"
     exon            881..1019
                     /note="larval exon 1"
     exon            914..1019
                     /note="adult exon 1b"
     intron          1020..1082
                     /number=1
     exon            1083..1487
                     /number=2
     intron          1488..1547
                     /number=2
     exon            1548..1963
                     /number=3
     polyA_signal    1940..1945
     polyA_site      1963
     CDS             join(927..1019,1083..1487,1548..1814)
                     /EC_number="1.1.1.1"
                     /gene="Adh"
                     /product="alcoholdehydrogenase"
                     /codon_start=1
                     /translation="MSLTNKNVVFVAGLGGIGLDTSRELVKRNLKNLVILDRIDNPAA
                     IAELKAINPKVTITFYPYDVTVPVAETTKLLKTIFAQVKTIDVLINGAGILDDHQIER
                     TIAVNYTGLVNTTTAILDFWDKRKGGPGGIICNIGSVTGFNAIYQVPVYSGSKAAVVN
                     FTSSLAKLAPITGVTAYTVNPGITKTTLVHKFNSWLDVEPRVAEKLLEHPTQTSQQCA
                     ENFVKAIELNKNGAIWKLDLGTLEPITWTQHWDSGI"
BASE COUNT      973 a    795 c    793 g    911 t
ORIGIN      
        1 cgtagtgtat ttaaggcact gcacatgtcg aggaccacga ttattgtcta cagtgcagtc
       61 agtagttgtc aggtgcagca ggcggaccag catttcttta aatctactca atcgatcaag
      121 taagtaactg taatccgagt acagtcgacc catcccactc ggttctgcag taactcaaga
      181 aatttcttga tagcgcagtt ccagttgtaa aacgtcgttt aaaaaaacat ctcgtaggag
      241 cttgggttga aaaacgttcg agatgggcgt agaaaagttc aaatataaat tatcaaaaaa
      301 gagaaaatat cttcccaaag cagctgtcca ttttttgtta aagtgataga tcaaaaattc
      361 gtatgacgta tttcccaagt gctctttatg caaataattt cctcaattct aattctacaa
      421 gaagcacttt tggctcttgt ccagaaaaaa acaatttgtt tacacttttc ggttgataac
      481 agaggcggac cgacggctaa gtaacataac ggtcgcctgt taagtaatgc cctcactctc
      541 tgttagatgg ccgcgcgtgt cgactccgat agaccagcag acacgcagta gttttcctca
      601 agaacctgaa ctctaaacat agacataatt tacgatacct tgagactcaa ataaaaatac
      661 aagtatgagt ggtattctat tcctgattag actcgcacac acacatacga gtattaacat
      721 aaatttgccg gcaaataacc aaaacaaaac gaacgcaaac gatccgaaca cgtaatgcga
      781 gagataagaa acagaaaagc tcgacgtgaa agaagcttct ggcggaagct cttgccgaac
      841 tccctataaa tacgagacag aaaaccagaa taaatataat aacaaactgt tgaattgtcc
      901 catcgattcc cagatcaaaa gacagaatgt ctctcaccaa caagaacgtc gttttcgtgg
      961 ccggtctggg cggcattggc ctggacacca gccgggagtt ggtcaagcgt aatctgaagg
     1021 taagagagaa cgaattccat ggaatctatg gaatcctgaa tttaaaaatt catttatttt
     1081 agaacctggt catcctggat cgcattgaca atccggctgc cattgccgaa ctgaaggcaa
     1141 tcaatcccaa ggtgaccatc accttctatc cctacgatgt gactgtgccc gtcgctgaga
     1201 ccaccaagct cctgaagacc atctttgccc aggtgaagac aatcgatgtc ctgatcaacg
     1261 gtgctggcat cctggacgat catcagatcg agcgcaccat tgccgttaac tacacgggcc
     1321 tggtcaacac caccacagcc attctggact tctgggacaa gcgcaagggc ggcccaggcg
     1381 gcatcatttg caacattggc tccgtcaccg gtttcaatgc catctaccag gtgcccgttt
     1441 actccggctc caaggcggcg gtggttaact ttacctcctc cctggcggta agaaatccta
     1501 caactatcct actccccaaa actaaatatt tctttgaaat cccatagaaa ctggctccca
     1561 ttactggtgt cactgcttac actgtcaatc ctggcatcac taagaccact ctggtccaca
     1621 aattcaactc gtggctggat gtggagcccc gtgtggcgga gaagctgctc gagcatccca
     1681 cccagacctc tcagcagtgc gccgagaact ttgtgaaggc cattgagctg aacaagaacg
     1741 gtgccatctg gaagttggat ttgggcacct tggagcccat cacatggacc cagcactggg
     1801 actcgggcat ctaaaccatc ccagagactc tatgggacat ggcgttttag cttttagttt
     1861 ctttttttcc actcaattgt tacgtatata catacacata tggcaataag gctgatttga
     1921 accccgtttt gaatatgata atacaaaatt atatttgaga aatttcaaca aaatcgataa
     1981 gagctctagc tctcttttaa ttaaaaaaaa ctagataaaa agagcaatgg tcagtggtgg
     2041 tcagtggtcg ttgtggcctc tatcgatttc cacacaaaaa ctttactgtt tagtaataga
     2101 aaagaacttg agaaaggcag ccaaaagaat gtacgatctg acgggtaagc atgtctgcta
     2161 tgtggctgac tgcggtggca ttgcactgga gaccagcaag gttctcatga ccaagaatat
     2221 agcggtgagt ggtagagtgg tgtagagagt ggagagtggt tctggttctt aaacaagtct
     2281 ctagtctcta gtctctagtc tcttggctgc tgctggtagc tgtcgaaact aattaagtta
     2341 atgacttttt catgttttgt ttttgtccgc tgacttttgc tgttcaaagc tacaattaag
     2401 tttatgtttt gagtttgatc tacttttgct taagggaagt actttcgatt acggattgct
     2461 ggcatataaa aatagatacc aaggaatctg catcatatca tattgattct atcttgattc
     2521 tttgcccaga aactggccat ccttcagagc gtggaaaacc ccccggccat cgctcagtta
     2581 caatcgatta agcacagcac acagatcttc ttctggacct tcgatgtgac catggcccgc
     2641 gaggagatga agaagtactt cgatgaggtc atggtccaga tggactacat cgatgtgctg
     2701 atcaatgggg ccaccctgtg cgatgagcgg aacattgatg ccaccatcaa tacgaatctg
     2761 accggcatga tgaacaccgt ggccactgtg ctgccctata tggaccgtaa gatgggcggt
     2821 tcgggtggac tgattgtgaa tgtcacctcc gtcataggac tggatccatc gccggtcttt
     2881 tgtgcgtaca gtgcctcaaa gtttggtgtg attgggttca cccgaagtct agcggtgagt
     2941 gggcgagatc ttaacttgaa aatcctctct aatgtcttcc attctttcgc aggatcccct
     3001 gtattacacc caaaatggtg tggctgtcat ggccgtctgc tgtggtccca ccaaagtgtt
     3061 tgtcgatcgg gaactgactg ccttcctgga atacggccaa acctttgccg atcgcctgcg
     3121 ccgtgccccc tgccaatcga cggccgtctg cggccagaat atagtaaatg ccatcgagag
     3181 atcggagaac ggacagatct ggattgccga caagggcggc ctggagtcgg tggccctcca
     3241 ctggtattgg catatggccg atcagtttgt gaactatatg cagagcactg acgatgagga
     3301 tcaggagttt tttttgggcc agcgatgagg catgccttga tccacggaat aaaacatcaa
     3361 atgtgtcaca aaaagtaggc aacttgtttg tgggtctgga aatagatttg aaatattttg
     3421 aggtcatatg aataatgaca taaatgaggg tgcctgaaag tatgccaagt tt
//