From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: E.coli (pUC18T) RNaseT (rnt) and RNA helicase-like protein genes,
Date: 11 Sep 92 10:03:35 GMT
Distribution: bionet
Checksum: 48309     4

LOCUS       ECORNTASET   1323 bp ds-DNA             BCT       11-SEP-1992
DEFINITION  E.coli (pUC18T) RNaseT (rnt) and RNA helicase-like protein genes,
            complete cds.
ACCESSION   L01622
KEYWORDS    RNA helicase-like protein; RNaseT; rnt gene.
SOURCE      Escherichia coli (strain K-12) DNA.
  ORGANISM  Escherichia coli
            Prokaryotae; Gram-negative facultatively anaerobic rods;
            Enterobacteriaceae.
REFERENCE   1  (bases 1 to 1323)
  AUTHORS   Huang,S. and Deutscher,M.P.
  TITLE     Sequence and transcriptional analysis of the Escherichia coli rnt
            gene encoding RNaseT
  JOURNAL   J. Biol. Chem. (1992) In press
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     -35_signal      130..135
                     /gene="rnt"
     -10_signal      153..158
                     /gene="rnt"
     mRNA            166..>1323
                     /gene="rnt"
     5'UTR           166..182
                     /gene="rnt"
     CDS             193..840
                     /product="RNaseT"
                     /gene="rnt"
                     /codon_start=1
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLSAAEEV"
     CDS             933..>1323
                     /product="RNA helicase-like protein"
                     /codon_start=1
                     /translation="MADNPDPSSLLPDVFSPATRDWFLRAFKQPTAVQPQTWHVAARS
                     EHALVIAPTGSGKTLAAFLYALDRLFREGGEDTREAHKRKTSRILYISPIKALGTDVQ
                     RNLQIPLKGIADERRRRGETEVNLRVGI"
BASE COUNT      310 a    374 c    345 g    294 t
ORIGIN
        1 actacggtta tcgcgtttgt ggaagatccg gacggttaca aaattgagtt aatcgaagag
       61 aaagacgccg gtcgcggtct gggcaactaa tctcctgccg ggcgtgaact catcgcgccc
      121 gcatctttac tgcatcgaca agtaatattt gtcataatgc gcgctgcaat ttatccgtat
      181 taagagaatc agatgtccga taacgctcaa cttaccggtc tgtgcgaccg ttttcgtggt
      241 ttttatcctg ttgtgatcga tgttgaaaca gccggattta acgccaaaac cgatgcgctg
      301 cttgagattg ccgccatcac cctgaaaatg gatgaacaag gctggctgat gccggacacc
      361 acattacatt tccacgtcga accatttgtc ggcgcaaatt tgcaaccaga agccctcgcc
      421 ttcaacggca ttgacccgaa cgatcccgat cgcggcgcgg tcagcgaata cgaggcgctg
      481 cacgaaattt ttaaagttgt acgtaaaggt attaaagcga gcggctgtaa ccgcgccatt
      541 atggtggcgc acaatgccaa ttttgatcac agctttatga tggccgccgc agaacgcgcc
      601 tcactgaaac gtaacccgtt ccaccctttc gccacttttg acactgctgc actggccggg
      661 ctggcactcg gacaaaccgt attgtcaaag gcttgccaga ccgctggcat ggacttcgac
      721 agcacccagg cgcactccgc gctgtacgac accgaacgca ctgctgtgct gttttgtgaa
      781 atcgtcaacc gctggaaacg tctgggaggc tggccgctat ctgccgccga agaggtgtaa
      841 tcgagtcgat gcctgatgac atgcaatgat tcaggcatct atagtgaggc tattccacgc
      901 atcctgcatg atattcacgg ggaatagcgt taatggcaga taatccagac ccttcatcgc
      961 tcctgccgga cgtgttttca ccggcgaccc gcgactggtt tcttcgcgcc tttaaacagc
     1021 cgaccgctgt ccagccgcaa acctggcatg tggcggcgcg aagcgaacat gcgctggtga
     1081 ttgcaccgac cggctccggg aaaacgctgg cagcatttct ctacgccctc gatcggctct
     1141 tccgcgaagg cggcgaagat acccgcgagg cgcataagcg taaaacctca cgcatcctct
     1201 atatttcacc gataaaagcc ctgggcaccg acgttcagcg caacttgcag atcccgttga
     1261 agggtattgc cgatgaacgg cggcggcgcg gcgaaacgga agtcaatctt cgcgtaggga
     1321 tcc
//