From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Yeast putative transcriptional activator (ADA2) gene, complete cds.
Date: 19 Aug 92 10:04:08 GMT
Distribution: bionet
Checksum: 14794     8

LOCUS       YSCADA2X     3798 bp ds-DNA             PLN       19-AUG-1992
DEFINITION  Yeast putative transcriptional activator (ADA2) gene, complete cds.
ACCESSION   M95396
KEYWORDS    .
SOURCE      Saccharomyces cerevisiae (strain yBP1) DNA.
  ORGANISM  Saccharomyces cerevisiae
            Eukaryota; Fungi; Eumycota; Hemiascomycetes; Endomycetales;
            Saccharomycetaceae.
REFERENCE   1  (bases 1 to 3798)
  AUTHORS   Berger,S.L., Pina,B., Silverman,N., Marcus,G.A., Agapite,J.,
            Regier,J.L., Triezenberg,S.J. and Guarente,L.
  TITLE     Genetic isolation of ADA2: A potential transcriptional adaptor
            required for function of certain acidic activation domains
  JOURNAL   Cell 70, 251-265 (1992)
  STANDARD  full staff_entry
FEATURES             Location/Qualifiers
     misc_feature    complement(1..310)
                     /function="ribosomal protein"
                     /standard_name="RP51B"
                     /note="The 5'end of the RP51B genomic clone was found to
                     overlap with the start codon for ADA2. The sequence
                     included does not contain any RP51B coding sequences."
                     /note="putative"
     CDS             308..1612
                     /function="involved in transcriptional activation"
                     /gene="ADA2"
                     /evidence=EXPERIMENTAL
                     /codon_start=1
                     /translation="MSNKFHCDVCSADCTNRVRVSCAICPEYDLCVPCFSQGSYTGKH
                     RPYHDYRIIETNSYPILCPDWGADEELQLIKGAQTLGLGNWQDIADHIGSRGKEEVKE
                     HYLKYYLESKYYPIPDITQNIHVPQDEFLEQRRHRIESFRERPLEPPRKPMASVPSCH
                     EVQGFMPGRLEFETEFENEAEGPVKDMVFEPDDQPLDIELKFAILDIYNSRLTTRAEK
                     KRLLFENHLMDYRKLQAIDKKRSKEAKELYNRIKPFARVMTAQDFEEFSKDILEELHC
                     RARIQQLQEWRSNGLTTLEAGLKYERDKQARISSFEKFGASTAASLSEGNSRYRSNSA
                     HRSNAEYSQNYSENGGRKKNMTISDIQHAPDYALLSNDEQQLCIQLKILPKPYLVLKE
                     VMFRELLKTGGNLSKSACRELLNIDPIKANRIYDFFQSQNWM"
                     /citation=[1]
     CDS             complement(1823..3142)
                     /note="open reading frame found c-terminal of ADA2 that
                     was sequenced during the cloneing of ADA2. There is no
                     information available regarding its function."
                     /note="putative"
                     /codon_start=1
                     /translation="MSKTRYYLEQCIPEMDDLVEKGLFTKNEVSLIMKKRTDFEHRLN
                     SRGSSINDYIKYINYESNVNKLRAKRCKRILQVKKTNSLSDWSIQQRIGFIYQRGTNK
                     FPQDLKFWAMYLNYMKARGNQTSYKKIHNIYNQLLSAPTNVDIWISCAKYEYEVHANF
                     KSCRNIFQNGLRFNPDVPKLWYEYVKFELNFITKLINRRKVMGLINEREQELDMQNEQ
                     KNNQAPDEEKSHLQVPSTGDSMKDKLNELPEADISVLGNAETNPALRGDIALTIFDVC
                     MKTLGKHYINKHKGYYAISDSKMNIELNKETLNYLFSESLRYIKLFDEFLDLERDYLI
                     NHVLQFWKNDMYDLSLRKDLPELYLKTVMIDITLNIRYMPVEKLDIDQLQLSVKKYFA
                     YISKLDSASVKSLKNEYRSYLQDNYLKKMNAEDDPRYKILDLIISKL"
BASE COUNT     1225 a    681 c    677 g   1215 t
ORIGIN
        1 atcaatcaat gcgcgtcttc cagatgaaat tcttcggaat gcccataaat acgaaaaact
       61 aacgatggaa aaaatactat aaatgtatgg gtgtaatttt aaccgaatgt atggatgtta
      121 cagggagctt tatatatgta gaaaattcca gacgttccaa acaaataagt gaaaatcaac
      181 acgattggga aagtacatgc cagagttcga tgtttaggtt aatgataaaa ataatttttc
      241 tctaatcacc ctccattttc gataaaatat cagcgtagtc tgaaaatata tacattaagc
      301 aaaaagaatg tcaaacaagt ttcactgtga cgtttgttca gctgattgca cgaacagagt
      361 gagggtttca tgtgctattt gtcccgaata cgacttatgc gtgccctgct tttcacaagg
      421 ctcgtataca ggaaagcatc gcccttacca tgattatagg ataatagaga ccaattcata
      481 ccccattctc tgtcctgatt ggggtgccga tgaagaattg cagttgatta aaggcgcaca
      541 gactttaggg ctcggtaatt ggcaggatat tgctgaccat ataggcagca gaggcaaaga
      601 agaagttaag gaacattacc taaaatatta tctggaaagc aaatactatc caatacctga
      661 tattacccaa aatatacatg tcccacaaga tgaatttttg gaacagcgaa ggcatagaat
      721 cgagtccttc cgggagaggc cgctagagcc tccaagaaag cccatggcat cggttcctag
      781 ctgccatgaa gtacaggggt ttatgccggg cagattagaa tttgaaacag aattcgaaaa
      841 tgaagctgaa ggtcccgtaa aggatatggt ttttgaacct gatgaccagc ccttagacat
      901 tgaattgaag tttgccatct tggacattta caattctaga ttgacaacca gagcagaaaa
      961 gaaaaggcta ttatttgaaa accatttaat ggattacaga aagttgcaag ctattgacaa
     1021 aaagaggagc aaagaagcca aagaattgta taaccgcata aaaccttttg ctcgtgttat
     1081 gactgcacag gattttgagg aatttagcaa ggatatatta gaagaattgc attgcagggc
     1141 aagaatacag caattacaag aatggagaag caatgggtta accacgttag aagcaggtct
     1201 taagtatgaa cgagataagc aagctagaat tagcagtttc gagaaatttg gcgcttctac
     1261 ggcggcgtca ctcagtgagg gcaatagccg ttataggtcg aattctgcac ataggtcgaa
     1321 cgcagaatac agccaaaact acagcgaaaa tggcggtaga aagaaaaata tgaccattag
     1381 cgatatacag catgcacctg actatgcact gttgtccaac gacgagcagc aactttgtat
     1441 tcaactgaaa attttaccta aaccgtatct cgttctaaaa gaggtaatgt tcagagaact
     1501 gttgaagact gggggtaatc taagcaaaag cgcatgtagg gaactactta atatagaccc
     1561 cataaaagcg aatagaatat acgatttttt ccagagccag aattggatgt aacaaaaaaa
     1621 aaattgaaaa gtaactacaa ttgtcactag ttattattgg ccaagtcata aagggacctt
     1681 gtctcgattt tatataccgt atatcggttc tttccaattt tttttttttt tttttttttt
     1741 tttttttttt ttggttgtaa tattaaaaga ataaacattt atctgatatg taattgcatt
     1801 tataaaatgt acagtaccgc atttaaagtt tgctgataat taaatctaga attttatacc
     1861 taggatcatc ttctgcattc atctttttca aataattgtc ttgtaaatat gaacgatact
     1921 catttttcaa tgacttcacc gatgcgctat ccaatttcga tatatatgca aaatacttct
     1981 taacagacaa ttgtaattga tcaatatcga gtttctcaac tggcatatat ctgatattta
     2041 gagtgatgtc aatcattact gttttcaaat acaattctgg tagatcctta cggagcgaaa
     2101 ggtcatacat gtcatttttc caaaactgca acacgtgatt gataagatag tctctttcca
     2161 agtctaaaaa ttcatcaaat agtttaatat accttagtga ctcactgaat aggtaattca
     2221 aggtctcctt atttagttca atattcattt tggaatctga gatggcatag taacccttat
     2281 gcttattaat ataatgcttg cccaaagtct tcatacacac atcgaaaata gttaatgcaa
     2341 tgtcaccgcg taatgctggg ttggtctctg cattacctag tacgctgatg tctgcctctg
     2401 gtaactcgtt taatttatct ttcattgaat caccagtgga tggaacttgt aaatgagatt
     2461 tttcttcgtc gggggcttgg ttattctttt gttcattttg catgtcgagc tcctgctctc
     2521 tttcattgat taaacccatc acttttcttc tgttaatcaa cttagtgatg aaattcagct
     2581 cgaattttac atattcatac catagcttag gcacatcagg gttaaacctc aacccattct
     2641 gaaaaatatt tctacaactc ttgaagttag catgaacttc atattcgtat ttcgcacaac
     2701 taatccaaat gtccacattc gttggtgcac ttagaagctg attataaatg ttgtgaattt
     2761 ttttgtatga tgtttggtta cctctcgctt tcatgtagtt caaatacatt gcccaaaact
     2821 ttaaatcttg tggaaatttg ttcgttcctc tttgataaat aaaacctatt ctttgttgaa
     2881 tagaccaatc agatagactg ttcgtctttt taacctgtaa gattctctta caccgcttgg
     2941 cacgtaattt attgacgtta ctttcatagt taatgtattt tatatagtca tttatgctag
     3001 accctctgga atttaatcta tgctcaaaat cagttctttt cttcataatt aatgaaactt
     3061 catttttagt gaatagtcct ttctccacca agtcatccat ttcaggaata cattgttcca
     3121 aatagtatct tgtcttcgac attattagaa atcggtgatg tgtgtatctc tggtcaattt
     3181 cacaatgtta tgcccttaga tatgtgttta ttcacgttca ctgtgtgttc ctgatattta
     3241 gctcatcgcc cagatctttc taaaaaattt tcaagagaga atacccggat gaaaaagcgt
     3301 gttcggattc ccatcactta atgaagctta tatctgacag cgcaggaaaa gcacaaaata
     3361 gaacctagcc acttcgcgta aaagctcata atgttaacga tatagtaata ttttgacgca
     3421 tcgaacttta tcacccgcag cataaagtac tgatactaca agtttttttc gttattctta
     3481 ccggtattgc gctaaagcag ttttgcacct gaaaacaagt aaaaatagga cttgaaaaaa
     3541 ttaaatagca tacatgtata atataatctt gatcacggca agtgtttcaa tagagatgtg
     3601 gttataattt gtcacttgtg cagtcaatac cttaaattaa cttttacaaa gtattttttt
     3661 taaacggccc atgagcataa attgagaacg atattattaa ttctagtata tttttaggaa
     3721 cagcaggcca ttatatccaa aactttttta aaatagcatc cacatcactt actggcgtac
     3781 agtttcgtgt ctggcaag
//