HDTenEightyP/NetNews huggingface dataset:
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Ginkgo biloba copia-like retrotransposon reverse transcriptase
Date: 14 Aug 92 10:00:29 GMT
Distribution: bionet
Checksum: 25022     2

LOCUS       GBICOPIA      274 bp ds-DNA             PLN       14-AUG-1992
DEFINITION  Ginkgo biloba copia-like retrotransposon reverse transcriptase
            gene, partial cds.
ACCESSION   M94475
KEYWORDS    copia-like element; retrotransposon; reverse transcriptase.
SOURCE      Ginkgo biloba DNA.
  ORGANISM  Ginkgo biloba
            Eukaryota; Plantae; Embryobionta; Pinophyta; Pinicae; Ginkgoatae;
            Ginkoaceae.
REFERENCE   1  (bases 1 to 274)
  AUTHORS   Voytas,D.F., Cummings,M.P., Konieczny,A., Ausubel,F.M. and
            Rodermel,S.R.
  TITLE     Copia-like retrotransposons are ubiquitous among plants
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 89, 7124-7128 (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     CDS             6..269
                     /note="PCR product"
                     /product="reverse transcriptase"
                     /codon_start=1
                     /translation="KTAFLNGVIEEEVYIEQPQGFEAHGRESHVCRLKKALYGLKQAP
                     RAWYSRIDTYLHQLGFEKSEADSNLYYILVGDDPLILVLYVDDM"
                     /partial
BASE COUNT       76 a     55 c     66 g     77 t
ORIGIN
        1 atgtaaagac cgcttttttg aatggtgtta ttgaagaaga ggtgtacatc gaacaacctc
       61 aaggctttga ggcacatggg agagagtctc atgtctgtag actaaagaaa gcactttacg
      121 gactcaaaca agctcctaga gcttggtact cacggatcga cacttatttg catcagttgg
      181 gctttgagaa gagtgaggcg gattccaacc tctactacat tctggttggt gatgatcccc
      241 tcattcttgt actatatgta gacgacatgg atcc
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human Ig germline H-chain gene V region, partial cds.
Date: 22 Aug 92 10:00:43 GMT
Distribution: bionet
Checksum: 24093     2

LOCUS       HUM9IGVH      434 bp ds-DNA             PRI       22-AUG-1992
DEFINITION  Human Ig germline H-chain gene V region, partial cds.
ACCESSION   M99405
KEYWORDS    immunoglobulin heavy chain subgroup VH-III;
            immunoglobulin heavy chain variable region.
SOURCE      Homo sapiens (individual_isolate LS) blood DNA.
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 434)
  AUTHORS   Adderson,E.E., Carroll,W.L. and Shackelford,P.G.
  TITLE     The human VH3b gene subfamily is highly polymorphic
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     CDS             join(1..13,117..434)
                     /product="immunoglobulin heavy chain variable region"
                     /gene="IGHV@"
                     /codon_start=1
                     /translation="AILKGVQCEVQLVESGGGLVKPGGSLRLSCAASGFTFSNAWMSW
                     VRQAPGKGLEWVGRIKSKTDGGTTDYAAPVKGRFTISRDDSKNTLYLQMNSLKTEDTA
                     VYYCTTDT"
                     /partial
     sig_peptide     1..13
                     /gene="IGHV@"
                     /codon_start=1
                     /partial
     intron          14..116
                     /note="'human germline immunoglobulin VH3 intron'"
                     /gene="IGHV@"
BASE COUNT      115 a     83 c    133 g    103 t
ORIGIN
        1 gctattttaa aaggtgattt atggagaact agagagatta agagtgagtg gatatgagtg
       61 agagaaacag tggatatgtg tggcagtttc tgatcttagt gtctctgtgt ttgcaggtgt
      121 ccagtgtgag gtgcagctgg tggagtctgg gggaggcttg gtaaagcctg gggggtccct
      181 tagactctcc tgtgcagcct ctggattcac tttcagtaac gcctggatga gctgggtccg
      241 ccaggctcca gggaaggggc tggagtgggt tggccgtatt aaaagcaaaa ctgatggtgg
      301 gacaacagac tacgctgcac ccgtgaaagg cagattcacc atctcaagag atgattcaaa
      361 aaacacgctg tatctgcaaa tgaacagcct gaaaaccgag gacacagccg tgtattactg
      421 taccacagac acag
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Lentinus strigosus ribosomal RNA (23LR3) large subunit, partial.
Date: 19 Aug 92 10:02:08 GMT
Distribution: bionet
Checksum: 10438     2

LOCUS       LENRRLSI      123 bp ss-rRNA            RNA       19-AUG-1992
DEFINITION  Lentinus strigosus ribosomal RNA (23LR3) large subunit, partial.
ACCESSION   M98591
KEYWORDS    large subunit ribosomal RNA.
SOURCE      Lentinus strigosus (library: Duke culture collection 635) cDNA to
            rRNA.
  ORGANISM  Lentinus strigosus
            Eukaryota; Fungi; Eumycota; Basidiomycotina; Hymenomycetes;
            Agaricales; Tricholomataceae.
REFERENCE   1  (bases 1 to 123)
  AUTHORS   Hibbett,D.S. and Vilgalys,R.
  TITLE     Phylogenetic relationships of the basidiomycete genus Lentinus
            inferred from molecular and morphological characters
  JOURNAL   Unpublished (1992)
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     rRNA            <1..>123
                     /note="Aligns to region between positions 371 to 597
                     inSaccharomyces cerevisiae large subunit nuclear rRNA."
                     /product="large subunit ribosomal RNA"
                     /citation=[1]
BASE COUNT       27 a     22 c     38 g     36 t
ORIGIN
        1 tgtattttct ggttgacggg ccagcatcaa ttttgaccgc tggaaaaagg ctatgggaat
       61 gtggcacctt ttggtgtgtt atagcccatg gtcatataca gcggttggga ttgaggaccg
      121 cag
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human chromosome 4 sequence-tagged site STS4-111
Date: 21 Aug 92 10:06:39 GMT
Distribution: bionet
Checksum: 14038     3

LOCUS       HUM4STS111    410 bp ds-DNA             PRI       21-AUG-1992
DEFINITION  Human chromosome 4 sequence-tagged site STS4-111
ACCESSION   M95255
KEYWORDS    STS; human chromosome 4; sequence tagged site.
SOURCE      Homo sapiens, clone C4-161 from Los Alamos National Laboratory
            chromosome 4 cosmid library, plate 4-1-2R, pos 11A; vector SuperCos
            1 (Stratagene)
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 410)
  AUTHORS   Goold,R.D., diSibio,G., Dugaiczyk,A., Smith,K.A., Xu,H.,
            Magrane,G., Lang,D.B., Cox,D.R., Masters,S.B. and Myers,R.M.
  TITLE     Sequence-Tagged Sites for Human Chromosome 4
  JOURNAL   Unpublished (1992) see COMMENT for author address
  STANDARD  full automatic
COMMENT     PCR components: 25 ng of human genomic DNA, 10 pmol of each
            oligonucleotide, 200 micro-M dNTPs, 0.25 U Taq  polymerase (Cetus)
            in 10 micro-l of 50 mM KCl-20 mM  Tris-HCl, pH 8.3 (at room temp),
            2.5 mM MgCl-2.  Initial denaturation at 94degC for 1.5 min, then 30
            cycles of  94degC for 15 sec, 62degC for 23 sec, and 72degC for 30
            sec, followed by a final extension at 72degC for 3.5 min, using a
            Perkin-Elmer 9600 thermocycler. PCR-amplified product size 183 bp.
            Amplified band runs with an apparent size of 200 bp.
            Sequence submitted by:
            Human Genome Mapping Center
            Box 0925
            University of California San Francisco
            San Francisco, CA 94143-0925  USA
            Phone: (415) 502-1612   Fax: (415) 476-8391
            e-mail: hgmcprobes@cgl.ucsf.edu
FEATURES             Location/Qualifiers
     misc_feature    38..61
                     /label=Primer1
     misc_feature    complement(195..220)
                     /label=Primer2
BASE COUNT       86 a    108 c     44 g    127 t     45 others
ORIGIN
        1 ttncnnnngc cttnacnggc catttggaat tgtcttttta ggactttcca attcacaccc
       61 tttgcccatt ttcctattgg tttctgttgt ctttttcata ttaattttag agctgtttat
      121 atatttatta taggttatta accattaagt atcatctgca gaaaaaagta caaaaattat
      181 ttcccaatat tttaaagttg tatgacatat tttggtgcaa ttccattnng gggnaataca
      241 antaaatatg tcttncatgg ctttncccct cttcccnngg ngacagagcc ncactctntc
      301 anccagnctn gngtncantc ctnanccacc ncccctaagc cccacccccc ntcccnnccn
      361 ncnccnacnt cnctctnnct ttccnnccan cncctccacn ccanctccan
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human chromosome 4 sequence-tagged site STS4-22
Date: 21 Aug 92 10:07:49 GMT
Distribution: bionet
Checksum: 62246     3

LOCUS       HUM4STS22     341 bp ds-DNA             PRI       21-AUG-1992
DEFINITION  Human chromosome 4 sequence-tagged site STS4-22
ACCESSION   M95320
KEYWORDS    STS; human chromosome 4; sequence tagged site.
SOURCE      Homo sapiens, clone L4-15 from the C25 lambda library (Pritchard,
            Genomics 4, 408, 1989); vector - Lambda Dash II (Stratagene)
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 341)
  AUTHORS   Goold,R.D., diSibio,G., Dugaiczyk,A., Smith,K.A., Xu,H.,
            Magrane,G., Lang,D.B., Cox,D.R., Masters,S.B. and Myers,R.M.
  TITLE     Sequence-Tagged Sites for Human Chromosome 4
  JOURNAL   Unpublished (1992) see COMMENT for author address
  STANDARD  full automatic
COMMENT     PCR components: 25 ng of human genomic DNA, 40 pmol of each
            oligonucleotide, 200 micro-M dNTPs, 0.25 U Taq  polymerase (Cetus)
            in 10 micro-l of 50 mM KCl-20 mM  Tris-HCl, pH 8.3 (at room temp),
            2.5 mM MgCl-2.  Initial denaturation at 94degC for 1.5 min, then 30
            cycles of  94degC for 15 sec, 62degC for 23 sec, and 72degC for 30
            sec, followed by a final extension at 72degC for 3.5 min, using a
            Perkin-Elmer 9600 thermocycler. PCR-amplified product size 341 bp.
            Sequence submitted by:
            Human Genome Mapping Center
            Box 0925
            University of California San Francisco
            San Francisco, CA 94143-0925  USA
            Phone: (415) 502-1612   Fax: (415) 476-8391
            e-mail: hgmcprobes@cgl.ucsf.edu
FEATURES             Location/Qualifiers
     misc_feature    1..24
                     /label=Primer1
     misc_feature    complement(317..341)
                     /label=Primer2
BASE COUNT      111 a     75 c     64 g     91 t
ORIGIN
        1 gaattcaacc catgaagtct agtgtcaatc ctttttacaa cctcccgcct ctgctcattc
       61 cgagactatc atgtttatgt ctgagattcc cagccaaagg ctttggctag agtctggcca
      121 cagaacataa ttgcttcaga tgatgttata aactgcaaca attaattgag cagatattta
      181 ataagcatcc aactatgaca gcatggtaga tagtaaacca tgcagttgaa taagataaca
      241 tggctccaac ccttgagaag ctcataacac agtgggacct ctaaatacag tgccagtctg
      301 ggtgaccaaa aatgcagagt gtataaatca gttatctcag a
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Pleurotus eryngii ribosomal RNA (20LR3) large subunit, partial.
Date: 19 Aug 92 10:03:18 GMT
Distribution: bionet
Checksum: 37155     2

LOCUS       POSRRLSA      240 bp ss-rRNA            RNA       19-AUG-1992
DEFINITION  Pleurotus eryngii ribosomal RNA (20LR3) large subunit, partial.
ACCESSION   M98588
KEYWORDS    large subunit ribosomal RNA.
SOURCE      Pleurotus eryngii (library: Duke culture collection 625) cDNA to
            rRNA.
  ORGANISM  Pleurotus eryngii
            Eukaryota; Fungi; Eumycota; Basidiomycotina; Hymenomycetes;
            Agaricales; Tricholomataceae.
REFERENCE   1  (bases 1 to 240)
  AUTHORS   Hibbett,D.S. and Vilgalys,R.
  TITLE     Phylogenetic relationships of the basidiomycete genus Lentinus
            inferred from molecular and morphological characters
  JOURNAL   Unpublished (1992)
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     rRNA            <1..>240
                     /note="Aligns to region between positions 371 to 597
                     inSaccharomyces cerevisiae large subunit nuclear rRNA."
                     /product="large subunit ribosomal RNA"
                     /citation=[1]
BASE COUNT       66 a     38 c     73 g     63 t
ORIGIN
        1 agggaaagat gaaaagaact ttggaaagag agttaaacag tacgtgaaat tgttgaaagg
       61 gaaacgcttg aagtcagtcg cgtctgccag ggatcaacct tacttttgtg aggcgtactt
      121 cctggttgat gggtcagcat cagttttggt tgctggataa aggtcagaga aatgtggcac
      181 cttcgggtgt gttatagtct ttgatcagat acagtggctg ggactgagga actcagcgcc
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human immunodeficiency virus type 2 (FOENVC34) envelope protein
Date: 18 Aug 92 10:01:59 GMT
Distribution: bionet
Checksum: 47761     2

LOCUS       HIVENVC34     453 bp ss-RNA             VRL       18-AUG-1992
DEFINITION  Human immunodeficiency virus type 2 (FOENVC34) envelope protein
            fragment.
ACCESSION   M87098
KEYWORDS    envelope protein.
SOURCE      Human immunodeficiency virus type 2 uncultured blood mononuclear
            cells (3/90 sample) RNA.
  ORGANISM  Human immunodeficiency virus type 2
            Viridae; ss-RNA enveloped viruses; Positive strand RNA virus;
            Retroviridae; Lentivirinae.
REFERENCE   1  (bases 1 to 453)
  AUTHORS   Gao,F., Yue,L., White,A.T., Pappas,P.G., Barchue,J., Hanson,A.P.,
            Greene,B.M., Sharp,P.M., Shaw,G.M. and Hahn,B.H.
  TITLE     Human infection by genetically-diverse SIVsm-related HIV-2 in West
            Africa
  JOURNAL   Nature (1992) In press
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     misc_feature    1..453
                     /note="PCR derived HIV-2 envelope fragment"
                     /gene="env"
                     /note="putative"
                     /citation=[1]
BASE COUNT      172 a     78 c     93 g    110 t
ORIGIN
        1 gacgtggtca aaagacaaca agaattgttg cgactgaccg tctggaggac taaaaacctc
       61 cagactagag tcactgctat cgagaagtac ctaaaggacc aggcgcagct aaattcatga
      121 agatgcgcgt tcagacaggt ctgccacacc actgtagaat ggccaaatag tactctcaca
      181 cctgactaga acaatatgac ttggcaggag taagaaagac aggttgattt cctagaggca
      241 aatataacac aattattaga agaggcacaa attcaacaag aaaagaacat gtatgagcta
      301 caaaaactaa ataactagga tatatttggc aactggtttg accttacttc ttagattaag
      361 tatatacagt atggagtctt catagtagta agtataatac tgttaagaat aataatctat
      421 gtagtacaaa tgttagctaa gttaagtaaa gga
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human chromosome 4 sequence-tagged site STS4-243
Date: 21 Aug 92 10:08:05 GMT
Distribution: bionet
Checksum: 35516     3

LOCUS       HUM4STS243    460 bp ds-DNA             PRI       21-AUG-1992
DEFINITION  Human chromosome 4 sequence-tagged site STS4-243
ACCESSION   L00757
KEYWORDS    STS; human chromosome 4; sequence tagged site.
SOURCE      Homo sapiens, clone p4-185 from a chromosome 4 plasmid library;
            vector pBluescript II KS + (Stratagene)
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 460)
  AUTHORS   Goold,R.D., diSibio,G., Dugaiczyk,A., Smith,K.A., Xu,H.,
            Magrane,G., Lang,D.B., Cox,D.R., Masters,S.B. and Myers,R.M.
  TITLE     Sequence-Tagged Sites for Human Chromosome 4
  JOURNAL   Unpublished (1992) see COMMENT for author address
  STANDARD  full automatic
COMMENT     PCR components: 25 ng of human genomic DNA, 10 pmol of each
            oligonucleotide, 200 micro-M dNTPs, 0.25 U Taq  polymerase (Cetus)
            in 10 micro-l of 50 mM KCl-20 mM  Tris-HCl, pH 8.3 (at room temp),
            2.5 mM MgCl-2.  Initial denaturation at 94degC for 1.5 min, then 30
            cycles of  94degC for 15 sec, 62degC for 23 sec, and 72degC for 30
            sec, followed by a final extension at 72degC for 3.5 min, using a
            Perkin-Elmer 9600 thermocycler. PCR-amplified product size 176 bp.
            Sequence submitted by:
            Human Genome Mapping Center
            Box 0925
            University of California San Francisco
            San Francisco, CA 94143-0925  USA
            Phone: (415) 502-1612   Fax: (415) 476-8391
            e-mail: hgmcprobes@cgl.ucsf.edu
FEATURES             Location/Qualifiers
     misc_feature    56..81
                     /label=Primer1
     misc_feature    complement(207..231)
                     /label=Primer2
BASE COUNT      187 a     51 c     75 g    139 t      8 others
ORIGIN
        1 gatctgccca cctcggcctt ccaaacttct gggattacaa gtgtaagcaa ccatgcccgc
       61 ttgattgatt gattatttta ctgtaaaata aaaagtctca gtagttttta aataatcatt
      121 tnggntgaat gatacctata gtagaaaact aaaccaaatg ctgaaataat taaagatatt
      181 ttcaatgaga aaaataactg attaatgatg ttttgacagt aggctacaaa aaaatggaat
      241 atatattaaa gagtggtaaa ctgtatttng gnagaattat gaaaatattt ttatatatat
      301 aataatattc tgctgagaaa agtatatcta gtatatggnc annctaaagc aaaatattaa
      361 gagatgagaa acataaaatg aaaaaaaatt taaattcata agggtttaca cacattatga
      421 aaaagtgttt ggnaaatatt tgggctgaac aagatagaca
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human chromosome 4 sequence-tagged site STS4-544
Date: 21 Aug 92 10:11:00 GMT
Distribution: bionet
Checksum: 60883     3

LOCUS       HUM4STS544    434 bp ds-DNA             PRI       21-AUG-1992
DEFINITION  Human chromosome 4 sequence-tagged site STS4-544
ACCESSION   L00915
KEYWORDS    STS; human chromosome 4; sequence tagged site.
SOURCE      Homo sapiens, clone C4-85 from Los Alamos National Laboratory
            chromosome 4 cosmid library, 1C7; vector SuperCos 1 (Stratagene)
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 434)
  AUTHORS   Goold,R.D., diSibio,G., Dugaiczyk,A., Smith,K.A., Xu,H.,
            Magrane,G., Lang,D.B., Cox,D.R., Masters,S.B. and Myers,R.M.
  TITLE     Sequence-Tagged Sites for Human Chromosome 4
  JOURNAL   Unpublished (1992) see COMMENT for author address
  STANDARD  full automatic
COMMENT     PCR components: 25 ng of human genomic DNA, 10 pmol of each
            oligonucleotide, 200 micro-M dNTPs, 0.25 U Taq  polymerase (Cetus)
            in 10 micro-l of 50 mM KCl-20 mM  Tris-HCl, pH 8.3 (at room temp),
            2.5 mM MgCl-2.  Initial denaturation at 94degC for 1.5 min, then 30
            cycles of  94degC for 15 sec, 62degC for 23 sec, and 72degC for 30
            sec, followed by a final extension at 72degC for 3.5 min, using a
            Perkin-Elmer 9600 thermocycler. PCR-amplified product size 194 bp.
            Sequence submitted by:
            Human Genome Mapping Center
            Box 0925
            University of California San Francisco
            San Francisco, CA 94143-0925  USA
            Phone: (415) 502-1612   Fax: (415) 476-8391
            e-mail: hgmcprobes@cgl.ucsf.edu
FEATURES             Location/Qualifiers
     misc_feature    42..65
                     /label=Primer1
     misc_feature    complement(211..235)
                     /label=Primer2
BASE COUNT      150 a     82 c     70 g    122 t     10 others
ORIGIN
        1 tcaagagaat ctngatggtg gcccttacaa aaaaggaact gctatttcat ccctccctaa
       61 agaccgcatc aaccccttgc tctgatattt gtatgacact gccagtttta gaagtttgtg
      121 attttatcag agaagagaca tttgaggcaa ctgatgaaac aaaaagataa ttatatttca
      181 taaagagaaa attaaaaaac ttcaaaatta ccaactgctg tttattgcta gaacagataa
      241 ttagttcatt tantgccttc cagcatttnt ncaaagcaga tagaaatggn ctcttattaa
      301 aggagttata aaaagctgtg ttatctggca tctaaccaac ttcccagatt ccacaaatnc
      361 ccaagttcac antagggtng agggaaaang ttacccatan ttccaagaca ggatcaaccg
      421 tttttccaat gtaa
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human chromosome 4 sequence-tagged site STS4-79
Date: 21 Aug 92 10:11:10 GMT
Distribution: bionet
Checksum: 02242     3

LOCUS       HUM4STS79     406 bp ds-DNA             PRI       21-AUG-1992
DEFINITION  Human chromosome 4 sequence-tagged site STS4-79
ACCESSION   M95231
KEYWORDS    STS; human chromosome 4; sequence tagged site.
SOURCE      Homo sapiens, clone C4-59 from Los Alamos National Laboratory
            chromosome 4 cosmid library, plate 4-1-1R, pos 5A; vector SuperCos
            1 (Stratagene)
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 406)
  AUTHORS   Goold,R.D., diSibio,G., Dugaiczyk,A., Smith,K.A., Xu,H.,
            Magrane,G., Lang,D.B., Cox,D.R., Masters,S.B. and Myers,R.M.
  TITLE     Sequence-Tagged Sites for Human Chromosome 4
  JOURNAL   Unpublished (1992) see COMMENT for author address
  STANDARD  full automatic
COMMENT     PCR components: 25 ng of human genomic DNA, 10 pmol of each
            oligonucleotide, 200 micro-M dNTPs, 0.25 U Taq  polymerase (Cetus)
            in 10 micro-l of 50 mM KCl-20 mM  Tris-HCl, pH 8.3 (at room temp),
            2.5 mM MgCl-2.  Initial denaturation at 94degC for 1.5 min, then 30
            cycles of  94degC for 15 sec, 62degC for 23 sec, and 72degC for 30
            sec, followed by a final extension at 72degC for 3.5 min, using a
            Perkin-Elmer 9600 thermocycler. PCR-amplified product size 257 bp.
            Sequence submitted by:
            Human Genome Mapping Center
            Box 0925
            University of California San Francisco
            San Francisco, CA 94143-0925  USA
            Phone: (415) 502-1612   Fax: (415) 476-8391
            e-mail: hgmcprobes@cgl.ucsf.edu
FEATURES             Location/Qualifiers
     misc_feature    51..75
                     /label=Primer1
     misc_feature    complement(283..307)
                     /label=Primer2
BASE COUNT      145 a     62 c     82 g    113 t      4 others
ORIGIN
        1 tttgatggaa tctaattagc aacaagttca gattctgaaa acatttngtt agactgacaa
       61 gagattagaa aggaaacaga tgatacttga gaaaggtcct ttccccccaa tatatatgta
      121 taatttccat tatgaagaat tagaatttca tacatggaac agtgaaaaaa tagaccagaa
      181 catattttct ttcttgaaaa gcactatttc agcagcaatg gaacagactg ctgaggtcag
      241 gagggtccca gagctagacg gtgtttgcga agccagggaa caggttttag tgtgaaagaa
      301 attctccgtg gccaaatgat ggaagtataa ggaaattatt ctgatgaata tgaaacatat
      361 tgagcattat ttactctcat cataaaatag tatnaccagc tntcgn
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human immunodeficiency virus type 2 (22ENVB4) envelope (gp 41)
Date: 18 Aug 92 10:01:08 GMT
Distribution: bionet
Checksum: 54064     2

LOCUS       HIV2ENVB4     453 bp ss-RNA             VRL       18-AUG-1992
DEFINITION  Human immunodeficiency virus type 2 (22ENVB4) envelope (gp 41)
            fragment.
ACCESSION   M87132
KEYWORDS    envelope protein; glycoprotein.
SOURCE      Human immunodeficiency virus type 2 uncultured blood mononuclear
            cells (5/89 sample) RNA.
  ORGANISM  Human immunodeficiency virus type 2
            Viridae; ss-RNA enveloped viruses; Positive strand RNA virus;
            Retroviridae; Lentivirinae.
REFERENCE   1  (bases 1 to 453)
  AUTHORS   Gao,F., Yue,L., White,A.T., Pappas,P.G., Barchue,J., Hanson,A.P.,
            Greene,B.M., Sharp,P.M., Shaw,G.M. and Hahn,B.H.
  TITLE     Human infection by genetically-diverse SIVsm-related HIV-2 in West
            Africa
  JOURNAL   Nature (1992) In press
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     misc_feature    1..453
                     /note="PCR derived HIV-2 envelope (gp 41) fragment"
                     /note="putative"
                     /citation=[1]
BASE COUNT      154 a     75 c    112 g    112 t
ORIGIN
        1 gacgtggtca aaagacaaca ggaaatgttg cggctgaccg tctggggaac taaaaacctc
       61 cagactagag tcactgccat cgagaagtac ctaaaggacc aagcaaaact aaattcatgg
      121 ggatgtgcct ttagacaggt ttgccacact actgtgccat gggtgaacga cagtatgcag
      181 ccagagtggc aaaacatgac ttggcaacaa tgggaaaaac agattgcctt cttggaggat
      241 aatatcacag agctgttaga gcaagctcaa atacagcagg agaaaaatat gtatgagttg
      301 cagaaattaa atagctggga tgtttttggc aattggcttg atctcacctc ttgggtaaaa
      361 tatatctatt taggatttta tatagtagca ggagtaatag tgttaagaat agctatctat
      421 gtagtacaaa tgttaatgag acttaggaag ggc
//