From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: N. crassa mitochondrial linear plasmid (maranhar) DNA for putative
Date: 24 Jul 92 10:08:49 GMT
Distribution: bionet
Checksum: 12992    13

LOCUS       NEUMTMIDRQ   7052 bp ds-DNA             ORG       24-JUL-1992
DEFINITION  N. crassa mitochondrial linear plasmid (maranhar) DNA for putative
            RNA and DNA polymerase.
ACCESSION   X55361
KEYWORDS    DNA polymerase; RNA polymerase.
SOURCE      Mitochondrion Neurospora crassa DNA.
  ORGANISM  Mitochondrion Neurospora crassa
            Eukaryota; Fungi; Eumycota; Ascomycotina; Pyrenomycetes;
            Sordariales; Sordariaceae.
REFERENCE   1  (bases 1 to 7052)
  AUTHORS   Court,D.A. and Bertrand,H.
  TITLE     A method for sequencing uncloned termini of linear plasmids.
  JOURNAL   Nucleic Acids Res. 19, 1714-1714 (1991)
  STANDARD  full automatic
COMMENT     *source: strain=Aarey-1e; *source: isolate=fungal genetic stock
            centre number 2499;
            May encode a DNA & RNA polymerase similar to phage polymerases.
            From EMBL    entry NCMIDRPM;  dated 04-APR-1991.
FEATURES             Location/Qualifiers
     repeat_region   1..349
                     /rpt_type=inverted
                     /rpt_type=terminal
     CDS             291..1169
                     /product="putative RNA polymerase"
                     /gene="ORF1"
                     /codon_start=1
                     /translation="MIKFYFTTFHMKFPARLFSTSFTLNNTNMMNEKDVKYITKNFLL
                     ENSNGFNLIKNMINSDETSEVKQNKIEVELNNIWHTEMTDILQKKRSLGLDAMGSSIL
                     AKDFHNLMGDMENFINKGRTNKLPGVEYLKPSLIISIVLGKVIPFSLRHSDIFNQPTH
                     SLFAEMGKMLKYQSIFELHHRIGLIQNRVEEIKNYTEKKLVSEFNKLTKVLDEYKNTL
                     KNLEELSGESIMKVGLGFTALLSETSEFYSLEEQIMAKNKSMRYMMPKNKLKTLINNM
                     TLMDTIELPMIMPPLE"
     CDS             complement(3697..6762)
                     /product="putative DNA polymerase"
                     /gene="ORF2"
                     /codon_start=1
                     /translation="MIKFYFTTFHMKFPARLFSTSGNRANFEENSSNEVQNQIKYLKD
                     FKKENPKFNWESTTEPKHMILSNIGEYEKNDFNLKSLLNINEFNINVELLRQLFMKLD
                     KNFTYALGVMLRNVDSNTNMSVDRHFLVNFNTDPVMILQQIYDRIIFLSEKYYLVPVD
                     KLFMKLRKLNFKVKNPVFHPMAKEKTNMHTTIPKGKSKLSRAEFVPHTMDLKFYGVET
                     KHDKEKNIRIFDNGKVILKVKIIKDGVYHLMDVISRDNRLLYQFEDVKHGDGLKRYWI
                     NDNMYYYYYDNTLVNVETPQKVGNMEPAKRDKTQDKKMLAFDIETFQVPTGNGDSTMI
                     AYACGFYDGKKSLTYYISDFISQREMLLACMKDMLKYDKHTVYCHNFSKFDINFIIKM
                     LVQEFVVEKMMSKDLDILSMKISYKFEPKKKGGKAERHTMTIADSCRLLPGSLDKLAK
                     DHNIITKKGKFPYKFVNKDNLEYVGLLPDYEYYIDPKKGEMITLFEWAAMYTNKWSMR
                     KETIIYLEKDMKALYQLMMEMSNNTYSTFRMNITRVKTASALAFLVYRTMFLPNEVEE
                     ENETNSPNNILSLFDKKEEKKLTPKYFLPKLKGRLERAVRAAYFGGRNEIFIPIMNNI
                     FSFDFNSLYPTAMMMPMPVGMPVHTFCKNLNEMFGFVRAKIITPAMNIPVLPCRVKVN
                     GVQKLIFPIGEWTGWYFSEELKLAVEYGYKMEVLESYVFEKRDDPFKEYIEHFASIKD
                     NTKGSKKQMAKLLLNTLYGRTGMNDSAAEIKMLTTNELDNIQLTNNVIHEFEVDDDKH
                     YVRYDKKPCPVLCAQSEKNYELLSYLDGEKDDGFIMNSTSIAAATASWSRILMYKHMI
                     NSAYTDTDSMFVEKPLDSAFIGEGCGKFKAEYNGQLIKRAIFMSGKLYLLDFGGKLEI
                     KCKGMTKNKDNTTHNLDINDFEALYNGESRVLFQERWGRSLELGTVTVKYQKYNLMSG
                     YDKREKLYSLGKWVNTSPLCMNENFEVISKALVSDVGESWYRKRMHYNK"
     repeat_region   complement(6704..7052)
                     /rpt_type=inverted
                     /rpt_type=terminal
BASE COUNT     2559 a    981 c    969 g   2543 t
ORIGIN
        1 ggggggatcg ggtaaagccc ttctttttaa tatgaacatc ccccttatag aggaaatagc
       61 ctatataaca ctatgagtga tcattagatc agtctatagt gttttactat ttcctcaatg
      121 tataacatgc gttttacctt tcccgcttat gcggctcttg acagagttgg ggcagatcca
      181 aagactacag tacgcacatc tccagacaga tccgggagcg aagcctacta ttacctgtca
      241 agaggagagc tgaaaaatag taggaatcgg ggtcacagaa tattttttta atgattaaat
      301 tttattttac cacatttcac atgaaatttc ctgcacgttt attttccact tcattcacat
      361 tgaataatac gaatataata aatgaaaaag atgttaaata tattactaaa aacttcttat
      421 tagaaaattc aaatggtttt aatttaatta aaaatataat taattctgat gaaacatctg
      481 aagttaaaca aaataaaatt gaagttgaat taaataatat ttggcatact gaaataactg
      541 acattttaca gaaaaaaaga agcttagggt tggatgcaat aggaagttct attttagcta
      601 aagattttca taatttaata ggagatatag agaattttat taataaagga agaacaaata
      661 agttaccagg cgttgaatac ttgaaaccat cattaattat ttctattgtc ttaggaaaag
      721 ttattccttt cagtcttaga cattcagata tttttaacca acctactcat tctttatttg
      781 cagaaatagg aaaaatatta aaatatcaat ctatttttga attacatcat agaattggtc
      841 ttattcaaaa tcgtgttgaa gaaattaaaa attatactga aaaaaaatta gtatctgaat
      901 ttaataaatt aaccaaagta ctagatgaat ataaaaacac tttaaaaaac ttagaagaat
      961 taagcgggga aagtattata aaagtaggat taggatttac agcactttta agtgaaacta
     1021 gtgaatttta ctctctcgaa gaacaaatta tagctaaaaa taaatcaata agatatataa
     1081 tacctaaaaa taaattaaaa acattaatta ataatataac attaatggat acaattgaac
     1141 tcccaatgat tataccacca cttgaatgaa aaattgatga taatgagaaa attattgaat
     1201 atggtggaac tatacttaat aataaacaca ggattagacc attaagaaca aaatcagtag
     1261 agaattcaga tgcaaatgat atgacatata ataaagagtt agttgatgcc gttaacttta
     1321 aatcaaaaat accatatact attaatttaa aacttttaga ttttattact agagatgaat
     1381 ttattaatcg tgataaaaaa gataatgtta ttatttataa acatattcat cctgactcgg
     1441 ctttattggg tgagtatatg aaggatcgta aaaacccaaa gattagtgaa ataacgactc
     1501 ataatagtaa atttctttat cattcttcta taatttcaat agcaaaatta atgaaagatg
     1561 ttaaggagtt ctatatgacg gtatttatag attgaagagg aagattttat acttcaagct
     1621 gtgcgcttaa catacaaggt ggggaattgg ctagaagttt acttctattt aaagaaggac
     1681 agaaattaaa tgatattgga ttaaaagctt taaaaatata tactgcaaat gctttcggtc
     1741 ttgataaaag atctaaagaa gaaagattgg attgagtcga acaaaatttg cataaaatta
     1801 ttgatataga caattatgaa atatgaagag aagctgatga accattacta tttttagcct
     1861 gtgccttgga gttgaaaggg tataatgagg accccaattt tatttcacat ttaccaattc
     1921 ttatggatgc aacatgtaat ggattacaac atttaagtgc aatggtaaat gattttgttt
     1981 tagcagaaaa agttaattta cttaagagta ctgaaaatga taatcctagg gatttatatt
     2041 ctgaagtaat tcctcatata aaacaagaaa ttttagaagc atcaaaatcg tacgaacaca
     2101 caaaccttga gcgtataaat gtagagagat gtctagttaa aaggggactt atgactatta
     2161 catatggagc aactgaaaga ggaatttatg atcaaattgt atctaaattc tttcaaaaag
     2221 atgaatgaaa caaaactgta gggcttcatt ttgtatgtat agattcggat attgctccaa
     2281 aagatgttgt atttactcaa aagaatatac ttcttttgag taaaattatt tataattctt
     2341 tatttaaaat tcatcctaat ttaaatactc taatggtata ttttaatagt atagttaaag
     2401 tattatgtga attagattta cctgttaatt gagtaactcc ttatggttta gtaatacaac
     2461 aaaaatataa taaatttact aaatataatg aaacaactta tgttgcatct aaacgatata
     2521 aattagtatt aagaaaagca gatactactt ctatttctaa gcagaaacaa atacaagcgt
     2581 ttatacccaa ttttgttcac tcaatggatg gaagtaatat tgttttatta ataaatacaa
     2641 taagagatga aggacgcaaa attaactttg caagtattca tgattgtttt gcaactcatg
     2701 caaatgatac tgcatgacta tcatgatatg taaagcaaag ttttatacgt atttattctg
     2761 attctagttt tttaagaaga tttcataatt atatccaatt aagaatccaa gctaaatatg
     2821 gagaagatgc tcattttgat aaagatggtg tattaaccca tgtaactatt gataatggta
     2881 cagatattcc tgatcgtatt gatataccta aacctcttgt agtaaataaa gataataaaa
     2941 tatataaaga aattcttcat tctgagtatt ttattaacta aatgataaat aatgattgta
     3001 aataatataa atatagtggg atttaacaaa aagggacaac acaaatagtg tatacccttt
     3061 tttaaaggga atacacaaat ttgtgtattc cctttttttt tttaaattat tatacggtta
     3121 agtaaataca catgtaataa caaagtaatg tttgtttatg ataggtagta cgtgcattgc
     3181 acccatgatc tacttcctct caaaatctta tattctactg aaggttaaat catagaaaaa
     3241 tacctatatt taatactgtt aagtatataa tttataatta taatatttaa tattaaataa
     3301 accaaaatta cacctgtcca gctcgtgaca agtactgtaa cgagataggt aaaaccctcg
     3361 aagggagaaa aataaaaaaa aagggaatgc aaaacctgca ttcccataaa aaaaaatagg
     3421 aatacacaat tcttttgtgt attccatttt tttttctttt ttaaaggttt acacaaatac
     3481 tgtgtatacc ttttttttaa agtatacaca aatactgtgt ataccttata gcgaaaagta
     3541 ttaatcaagg ttaaccaaat aacatttata atatattaaa ctaaataaca atcattacct
     3601 catattgtaa ataatggtgt gaggaaaata aaatgtaata ttagttgtta tactttagaa
     3661 tacttttaga taatcatata gacgcctggg ggggctctat ttattataat gtattctttt
     3721 tctatatcaa ctttcaccca catctgatac caaggctttt gaaattacct caaaattttc
     3781 gtttatacat aatggtgaag tattaactca tttacctaaa gagtatagtt tttcacgttt
     3841 atcatatccc gatataagat tatatttttg atatttaact gtaactgttc ctaattccaa
     3901 gctacgtcct catctttctt gaaatagtac cctactttct ccattataca gtgcttcgaa
     3961 atcattaata tctagattat gtgtggtatt atctttgttt ttagttattc ctttacattt
     4021 aatttctaat ttaccaccaa aatctaatag atataatttt cctgatatga aaattgcacg
     4081 tttaattaat tgtccgttat attcagcttt aaatttccca catccttctc caataaaagc
     4141 actatctaat ggtttttcca caaatattga atcagtatca gtatatgctg aattaattat
     4201 atgtttatac atcagaattc tagatcatga tgctgttgct gcagcaatgg atgtagagtt
     4261 tatgataaat ccatcatctt tctctccatc taggtaagat aaaagttcgt aatttttttc
     4321 tgattgtgca cacaatacag gacatggttt tttatcgtat ctcacgtaat gtttatcatc
     4381 atctacttca aattcgtgaa taacgttatt agttaattga atattatcta attcattagt
     4441 agttagcatt ttaatttcag cggcgctatc attcatacct gttcgaccat ataaagtgtt
     4501 caaaagcaat ttagccatct gtttctttga acctttagta ttatctttaa ttgaggcaaa
     4561 atgctcaata tattccttaa aaggatcatc tcttttttcg aaaacgtaac tctcaagtac
     4621 ttctatttta tatccgtact caactgctaa ttttaattct tcagaaaaat atcaccctgt
     4681 tcattcacca atgggaaaaa ttaatttttg tactccattg actttaactc tgcaaggtaa
     4741 tactggaata tttattgctg gagtaataat ttttgctctt acaaatccaa atatttcatt
     4801 taagttttta caaaaagtat ggactggtat accaacaggc attggcatca tcatggcagt
     4861 agggtaaaga ctattaaaat caaatgagaa aatattattt ataattggaa taaaaatttc
     4921 attacgtcca ccgaaataag cagctctaac ggctctttca agtcttcctt ttagtttagg
     4981 taaaaaatat ttaggagtta attttttctc ctcttttttg tcaaacaatg ataaaatgtt
     5041 attaggtgaa ttagtttcat tttcttcttc tacttcattt ggtaaaaata tcgttctata
     5101 aactaagaag gccaatgcag atgcggtttt aactctagta atatttattc taaaagttga
     5161 atatgtatta ttactcattt ccatcattaa ttgatataaa gcctttatat ctttttctaa
     5221 atagataatg gtttcttttc tcatcgatca tttattagta tacatagcag ctcactcaaa
     5281 tagtgtaatc atttcacctt ttttgggatc aatataatac tcataatcag gtaataatcc
     5341 aacatattct aaattatctt tattaacaaa tttgtaagga aattttcctt ttttagtaat
     5401 gatattatga tctttagcta atttatctag agatcccggt aggagtctac atgaatcggc
     5461 aattgttata gtatgccgct cagcttttcc tccttttttt ttaggttcaa atttatatga
     5521 aatttttatt gaaagaatat caagatcttt agatattatt ttttctacaa caaattcttg
     5581 aactaatatt ttgataataa aattaatatc aaatttggaa aaattatggc aatatactgt
     5641 atgtttgtca tattttaaca tatcttttat acatgctagt aacatttcac gttgagaaat
     5701 aaaatcagaa atgtaataag taagagattt ttttccatca taaaatccac aagcataagc
     5761 aatcatcgtt gaatcaccat tcccagtagg aacttgaaat gtttcaatat caaatgctaa
     5821 tattttttta tcttgtgttt tatctctttt cgctggctct atattaccaa ctttttgagg
     5881 tgtttcaaca ttgactaaag tattatcata gtaataataa tacatattat cattaattca
     5941 atatctttta agaccatcac catgcttaac atcttcaaac tggtacaata atctattatc
     6001 acgtgaaata acatctataa gatgatatac accatctttg ataattttca ctttaagaat
     6061 aacttttcca ttatcaaaaa tacgaatgtt tttttcttta tcatgttttg tttcaacacc
     6121 ataaaattta agatccattg tgtgtggaac aaattctgct ctagaaagtt tagactttcc
     6181 tttaggaata gtagtgtgta tattagtttt ttcttttgct ataggatgaa aaactggatt
     6241 tttaacttta aaattaagct ttcttaattt tataaaaagt ttatcaactg gcactaaata
     6301 gtatttttca gaaagaaaaa taattctatc ataaatttgt tgtaagatta taactggatc
     6361 agtattaaaa ttgactaaaa agtgtctgtc tactgatata ttagtattac tatcaacatt
     6421 tctaagtata accccaagtg cgtatgtaaa atttttatct aatttcatga atagttgacg
     6481 tagtaattca acattaatat tgaattcatt aatatttaaa agtgatttta aattaaaatc
     6541 atttttttca tattcaccaa tgttacttaa aatcatatgt ttaggttcgg tagtactttc
     6601 tcaattaaat ttagggtttt ctttcttaaa atctttcaaa tatttaattt gattttgtac
     6661 ttcatttgaa gaattttctt caaaattggc tcgatttcca ctcgtggaaa ataaacgtgc
     6721 aggaaatttc atgtgaaatg tggtaaaata aaatttaatc attaaaaaaa tattctgtga
     6781 ccccgattcc tactattttt cagctctcct cttgacaggt aatagtaggc ttcgctcccg
     6841 gatctgtctg gagatgtgcg tactgtagtc tttggatctg ccccaactct gtcaagagcc
     6901 gcataagcgg gaaaggtaaa acgcatgtta tacattgagg aaatagtaaa acactataga
     6961 ctgatctaat gatcactcat agtgttatat aggctatttc ctctataagg gggatgttca
     7021 tattaaaaag aagggcttta cccgatcccc cc
//