From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: V.proteolyticus neutral protease gene, complete cds.
Date: 9 Apr 92 10:05:32 GMT
Distribution: bionet
Checksum: 27972     5

LOCUS       VIBNEUP      2070 bp ds-DNA             BCT       09-APR-1992
DEFINITION  V.proteolyticus neutral protease gene, complete cds.
ACCESSION   M64809
KEYWORDS    neutral protease.
SOURCE      V.proteolyticus (ATCC 15338) DNA, clone pVP8.
  ORGANISM  Vibrio proteolyticus
            Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
            Facultatively anaerobic rods; Vibrionaceae.
REFERENCE   1  (bases 1 to 2070)
  AUTHORS   David,V.A., Deutch,A.H., Sloma,A., Pawlyk,D., Ally,A. and
            Durham,D.R.
  TITLE     Cloning, sequencing and expression of the gene encoding the
            extracellular neutral protease, vibriolysin, of Vibrio
            proteolyticus
  JOURNAL   Gene 112, 107-112 (1992)
  STANDARD  simple staff_entry
FEATURES             Location/Qualifiers
     -10_signal      21..27
     misc_feature    151..222
                     /note="leader sequence"
     CDS             151..1980
                     /product="neutral protease"
                     /codon_start=1
                     /translation="MNKTQRHINWLLAVSAATALPVTAAEMINVNDGSLLNQALKAQS
                     QSVAPVETGFKQMKRVVLPNGKVKVRYQQTHHGLPVFNTSVVATESKSGSSEVFGVMA
                     QGIADDVSTLTPSVEMKQAISIAKSRFQQQEKMVAEPATENEKAELMVRLDDNNQAQL
                     VYLVDFFVAEDHPARPFFFIDAQTGEVLQTWDGLNHAQADGTGPGGNTKTGRYEYGSD
                     FPPFVIDKVGTKCSMNNSAVRTVDLNGSTSGNTTYSYTCNDSTNYNDYKAINGAYSPL
                     NDAHYFGKVVFDMYKDWMNTTPLTFQLTMRVHYGNNYENAFWNGSSMTFGDGYSTFYP
                     LVDINVSAHEVSHGFTEQNSGLVYENMSGGMNEAFSDIAGEAAEFYMKGSVDWVVGAD
                     IFKSSGGLRYFDQPSRDGRSIDHASDYYNGLNVHYSSGVFNRAFYLLANKAGWDVRKG
                     FEVFTLANQLYWTANSTFDEGGCGVVKAASDMGYSVADVEDAFNTVGVNASCGATPPP
                     SGDVLEIGKPLANLSGNRNDMTYYTFTPSSSSSVVIKITGGTGDADLYVKAGSKPTTT
                     SYDCRPYKYGNEEQCSISAQAGTTYHVMLRGYSNYAGVTLRAD"
     mat_peptide     742..1977
                     /product="neutral protease"
                     /codon_start=1
BASE COUNT      565 a    469 c    516 g    520 t
ORIGIN
        1 aattgacgtg ggatataaaa tatttttctt acaaactgga atgttacaga aatgtaatta
       61 atggttattt cgcgagttat cgcaggaggg tttaatttct gatttatcag tagttaaaca
      121 acgattgaaa ataatctcca ggattgagaa atgaataaaa cacaacgtca catcaactgg
      181 ctgctggctg ttagcgcggc aactgcgcta cctgtcaccg ctgcagaaat gatcaacgta
      241 aatgatggca gcctgctaaa ccaggctctt aaagctcagt cacagagcgt tgccccggtg
      301 gaaaccggat tcaaacaaat gaaacgagtt gttttgccaa atggcaaagt gaaagttcgt
      361 tatcaacaaa ctcaccacgg tctaccggtt ttcaacacct cggtagtggc gactgaatcg
      421 aagtctggta gtagcgaagt gttcggtgtg atggctcagg gtatcgcaga cgacgtgtct
      481 acactgacgc catccgttga gatgaagcag gccatttcaa ttgctaaatc gcgtttccaa
      541 cagcaagaaa aaatggttgc ggaacctgca acggaaaacg aaaaagccga gttgatggtt
      601 cgtctggacg acaacaatca agcgcaacta gtgtatctgg ttgatttctt cgttgccgag
      661 gatcacccag cgcgtccttt ctttttcatt gatgcgcaaa cgggtgaagt actgcaaact
      721 tgggatggtc tgaaccatgc acaagctgac ggtactggcc ctggcggtaa caccaaaaca
      781 ggtcgttatg aatacggttc tgactttcct ccgtttgtca tcgataaagt cggcactaag
      841 tgttcaatga acaacagcgc ggtaagaacg gttgacctga acggctcaac ttcaggtaac
      901 accacttaca gctatacctg taacgactca accaactaca acgattacaa agccattaac
      961 ggcgcgtact cgccactgaa cgatgcccac tacttcggta aagtggtttt cgatatgtac
     1021 aaagactgga tgaacaccac accactgacg ttccagctga ctatgcgtgt tcactatggt
     1081 aacaactacg aaaacgcgtt ctggaatggt tcatccatga ccttcggtga tggctacagc
     1141 accttctacc cgctggtgga tattaacgtt agtgcccacg aagtgagcca cggtttcacc
     1201 gaacaaaact cgggtctggt gtacgagaat atgtctggtg gtatgaacga agcgttctct
     1261 gatattgcag gtgaagcagc agagttctac atgaaaggca gcgttgactg ggttgtcggt
     1321 gcggatatct tcaaatcatc cggcggtctg cgttactttg atcagccttc gcgtgacggc
     1381 cgttctatcg accatgcgtc tgactactac aatggcctga atgttcacta ctcaagtggt
     1441 gtattcaacc gtgcgttcta cctgctggct aacaaagcgg gttgggatgt acgcaaaggc
     1501 tttgaagtgt ttaccctggc taaccaattg tactggacag cgaacagcac atttgatgaa
     1561 ggcggttgtg gtgtagtgaa agctgcgagc gacatgggtt acagcgttgc agacgtagaa
     1621 gatgcgttta acacggtagg cgttaacgcg tcttgtggtg caactcctcc tccgtctggc
     1681 gatgtactgg aaatcggtaa accgctggcg aacctttcag gtaaccgcaa tgacatgact
     1741 tactacacgt tcacaccaag cagctcatct agcgtagtga ttaagatcac tggcggtaca
     1801 ggtgatgcag acctttacgt gaaagcgggt agcaagccaa ccacgacttc ttacgattgc
     1861 cgtccatata agtatggtaa cgaagagcag tgttcaattt cagcgcaagc gggtactacg
     1921 tatcacgtta tgctgcgtgg ttacagcaat tacgctggtg taactttgcg tgctgactaa
     1981 actcagaatg gaaccagtga aggcgcacct taaggtcgcc ttttttgtat caggcgatct
     2041 gtgtaaacgt gacctgatcg aagtgaggat
//