From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: K.pneumoniae genes of the pulC operon
Date: 5 Jan 93 06:47:05 GMT
Distribution: bionet
Checksum: 52581 10

LOCUS       KPNPULC      5082 bp ds-DNA             BCT       04-JAN-1993
DEFINITION  K.pneumoniae genes of the pulC operon
ACCESSION   X52462
KEYWORDS    pullulanase.
SOURCE      Klebsiella pneumoniae DNA.
  ORGANISM  Klebsiella pneumoniae
            Prokaryotae; Gram-negative facultatively anaerobic rods;
            Enterobacteriaceae.
REFERENCE   1  (bases 1 to 5082)
  AUTHORS   Pugsley,A.P. and Reyss,I.
  TITLE     Five genes at the 3' end of the Klebsiella pneumomiae pulC operon
            are required for pullulanase secretion
  JOURNAL   Mol. Microbiol. 4, 365-379 (1990)
  STANDARD  full automatic
COMMENT     From EMBL    entry KPPULC;  dated 18-DEC-1992.
FEATURES             Location/Qualifiers
     CDS             <1..608
                     /gene="pulK"
                     /codon_start=3
                     /translation="IFTRLLENLGSEPLRALQLTAALRDWVDDDRQPLLNGAEDEVYM
                     AQSPGYLTGNQPLQDVSELRLLAGMDAALYQRLLPYVCALADETLQVNVNTLQPDRAA
                     LLAALFPAELTLVEARQLLQARAATGWSSVAAFLSQPALQKTDTAAARPWLAVHSERF
                     IATFSVVMGNARYQQRSLLQKQGRTFGVVQRRYGIYWVADE"
     CDS             601..1797
                     /gene="pulL"
                     /codon_start=1
                     /translation="MNNHHTSSAAVLIIRLNPDAATAIWRLAAPGDTAQTGEWHPDAG
                     DPTLSLLAQRHPAWVLVPASDCAFHRVALPAGARRRPQQALAFLLEEQLATEVEESHF
                     ALLHQHKTDCAVAVVGRGKMRAWQAWCDSLGLSVLALTPDVLALPHSPTGWSAVRCGE
                     QWLFRCDTWGGMAVETVWLDQLLTHWQDLAPIACYSPPPDIAAPWQPLPAQDLLQLAA
                     ANPDARRICLRQGDFAAKRRRQPTPRRWRPVIVAALALLLLWSSNCLHDHLMLGQQAD
                     AAVQASRDFYRQWFQTEKNVVNPRLQMQQHLRQMKNAGARPALISRLGALQQIIDDTP
                     GIRLRTLSFDAARNALQLEISAVSSQALEQFSQRARARFRVQTGEMKPRADGIEGRLT
                     LEGNDA"
     CDS             1790..2275
                     /gene="pulM"
                     /codon_start=1
                     /translation="MHNLLALWQQRTRRERCLLLGMAVVLLIGLVYYTLWQPWQNREA
                     QWRQTLAREQASLQWMRQQTPLIRQLRNQKPPTAPEEPSTVIMREAARHGLTIVRLQP
                     QGSRLSLTVQPADFQALMAWLDALGQAGMTTATLAVTAVAQQPGWVTVNTLVLERSDE
                     K"
     CDS             2265..3023
                     /gene="pulN"
                     /codon_start=1
                     /translation="MKNRLTIGLLLAAIYLFWLLLSAPARLLALTLSDDARLAQTSGT
                     LWQGEAHQASWRGVELAYLRWEFGFSTWLPGWHIRFNDPSGLRGQAWLHGLNEFVVRE
                     GRLVIPARLISQRLALGMPLEARGQLALTLPEASFNANGCRRIAASAVQWQDAALSSP
                     AGLLELAQVNGKLSCTPAGALAVALTQDSHQLSLTGQGVLTPDGRYTFNGTLQPRQAA
                     PALLTLLVAQNGRKDEQGRIPWRWQGEWLSEEKK"
     CDS             3023..3709
                     /gene="pulQ"
                     /codon_start=1
                     /translation="MVENIALLPEFAAQYPFLWGSFLFLSGLAFGSFFNVVIHRLPLM
                     MEQAEGINLCFPASFCPQCREPIAWRDNIPLLGFLFLKGRSRCCGQPISPRYPLMELA
                     TGALFVLAGYLMAPGVPLLGGLILLSLLLILAAIDAQTQLLPDGLTLPLMWAGLLFNL
                     SATYVPLAEAVVGAMAGYLSLWSVYWVFRLLSGKEALGYGDFKLLAALGAWLGWQALP
                     QTLLLASPAA"
BASE COUNT      807 a   1713 c   1645 g    917 t
ORIGIN      
        1 agatctttac ccgcctgctg gaaaacctcg gcagcgagcc gctgcgcgcc ttacagctca
       61 ctgccgccct gcgcgactgg gtcgatgacg atcgccagcc gctgctgaac ggcgcggaag
      121 acgaggtcta tatggcgcaa tcccccggct atctgaccgg caatcagccg ctgcaggacg
      181 tcagcgagct gcgtctgctg gccgggatgg acgccgcgct ctatcagcgc ctgctgccct
      241 acgtctgcgc cctggcggac gagacgctgc aggtgaacgt caataccctg cagcccgatc
      301 gggccgcgct gctggcggcg ctgttccccg ctgaactcac cctcgtcgaa gcccggcagc
      361 tgctgcaggc ccgcgcggcc accggctgga gcagcgtcgc cgccttcctg tcgcagccgg
      421 cattacaaaa aaccgatacc gccgccgcgc gcccgtggct ggcggttcac agcgagcgtt
      481 ttatcgccac cttcagcgta gtgatgggca acgcccgcta tcagcaacgc agcctcctgc
      541 aaaagcaggg gcgtaccttc ggcgtcgtcc agcggcgcta cggaatctac tgggtagccg
      601 atgaataacc accatacctc ttccgccgcc gtgctgatta ttcgcctcaa tcccgatgcc
      661 gcgacggcga tatggcggct ggccgcgccg ggcgacacgg cgcagactgg cgagtggcat
      721 cctgacgctg gcgatccgac gctcagcctg ctggcgcagc gccatccggc ctgggtactg
      781 gtgcccgcca gcgattgcgc atttcatcgc gtcgctctgc ccgccggggc gcgcagaaga
      841 ccgcagcagg cgctggcgtt tttactcgaa gaacagctgg cgacggaagt tgaggagagc
      901 cactttgcct tgctccacca gcacaaaacc gactgcgccg tggcggtggt cgggcgggga
      961 aagatgcgcg cctggcaggc ctggtgcgac agcctcgggc tgagcgtact ggctctgacg
     1021 ccggatgtcc tggcgctgcc gcacagtccc accggctgga gcgccgtacg ctgcggcgag
     1081 cagtggctgt ttcgctgtga tacgtggggc ggaatggcgg tggaaaccgt atggctcgac
     1141 cagctgctga cccactggca agacctcgcg cccatcgcct gctactcgcc gccgccggat
     1201 atcgccgcgc cgtggcagcc tctcccggcg caggatctgc tgcagctggc ggcggccaat
     1261 cctgatgcgc gcagaatctg cttacgccag ggcgattttg ccgcaaaacg ccgccggcag
     1321 ccgacgccac gacgctggcg cccggtaatc gtcgcggcgc tggcgctact gctgctgtgg
     1381 agcagtaatt gcctccacga tcatctcatg ctgggacagc aggccgacgc cgccgtgcag
     1441 gccagccgtg atttttaccg ccagtggttc caaacggaaa agaacgtcgt caacccgcgc
     1501 ctgcaaatgc agcagcatct gcggcagatg aaaaacgcgg gcgcccggcc ggcgcttatc
     1561 tcgcggctcg gcgcgctgca gcaaatcatc gacgacacgc cgggcatccg cctgcggacg
     1621 ttgagctttg acgccgcgcg caacgcgctg cagctggaga tatccgccgt ttcctcgcag
     1681 gccctggagc agttcagcca gcgggcgcgg gcgaggtttc gcgtgcagac cggcgagatg
     1741 aagccgcgcg ccgacggtat tgaagggcgc ctgacgctgg aggggaacga tgcataacct
     1801 gctcgcctta tggcaacagc gtacgcgccg cgaacggtgt ctgctgctgg gtatggccgt
     1861 ggtactgctc atcggcctgg tctattacac gttatggcag ccgtggcaaa accgcgaggc
     1921 gcagtggcgg caaacgctgg ccagggagca ggccagcctg cagtggatgc gccagcagac
     1981 gccccttatc cgtcagcttc gcaatcagaa gccgcccacc gcgccggaag aaccctcgac
     2041 ggtgattatg cgcgaagccg cccgtcacgg tctcaccatc gttcgcctgc agccgcaggg
     2101 ctcccgcctg agccttaccg tgcagcccgc cgatttccag gcgttgatgg catggctgga
     2161 cgcgctgggg caggcgggga tgaccaccgc caccctggcg gtgaccgccg tagcgcagca
     2221 gcccggctgg gtgaccgtca atacgctggt gctggagcgc agcgatgaaa aatagactca
     2281 ctatcggcct gctgctggcg gcgatctatc tcttctggct gctgctcagc gcgccggcga
     2341 gactgctggc cctgacgcta tcggacgacg cgcggctcgc gcaaacgtcg ggcactctgt
     2401 ggcaaggaga ggctcatcag gccagctggc gcggcgttga attagcctat ttacgctggg
     2461 agtttggctt ctcgacctgg cttcccggct ggcatatccg ttttaacgat ccctccggcc
     2521 tgcgcggcca ggcctggctg cacggcctga acgagtttgt ggtgcgcgag ggacggctcg
     2581 tgattccggc ccgtttaatc agccagcggc tggcgctggg tatgccgctg gaagcccgcg
     2641 gccagctggc gctgacgctg ccggaggcca gctttaacgc caacggctgc cggcggatag
     2701 ccgcgagcgc cgtgcagtgg caagatgcgg cgctcagctc ccccgccggg ttgctcgaac
     2761 tggcccaggt caacggcaaa ttaagctgta ccccggcggg tgcgctggct gtcgccctga
     2821 cccaggactc ccatcagctt agcctcaccg gacagggcgt tctgacgccc gacgggcgct
     2881 atacgtttaa cggtacgctg caacctcgtc aggcggcccc ggcgctgctg acgctgctgg
     2941 tcgcgcaaaa cggccgtaaa gatgagcagg gacgcatacc gtggcggtgg caaggagagt
     3001 ggctatccga ggagaaaaaa taatggtcga aaacatcgcg ttattacccg agttcgccgc
     3061 gcaatacccg tttctctggg gcagcttttt attcctgtcc ggactggcgt tcggcagctt
     3121 ttttaacgtc gttatccacc gtctgccgct gatgatggag caggcagagg gaatcaatct
     3181 ctgttttccc gcgtcgttct gtccgcagtg ccgtgagccc atcgcctggc gggacaatat
     3241 tccgctactg ggcttcctgt ttcttaaggg gcgttcgcgc tgctgcgggc agccgatctc
     3301 gcctcgctat cctctgatgg agctggcgac cggcgcgctg tttgtgctgg cgggatacct
     3361 aatggcgccg ggcgtgccgc tgctgggcgg gttgatttta ttgtcgctgc tgctgatcct
     3421 cgccgccatc gacgcgcaaa cgcagctgct gccggacggg ctgacgctac cgctgatgtg
     3481 ggccgggctg ttgttcaatc ttagcgcgac ttacgtgccg ctggcggagg cggtcgtggg
     3541 cgcgatggcc gggtatttat cgctgtggtc ggtgtactgg gtattccgtc tgctgagcgg
     3601 taaagaggcg ctgggctacg gcgatttcaa actgctggcg gcgctcggcg cgtggctcgg
     3661 ctggcaggcg ctgccgcaga cgctgctgct ggcttcgcca gcggcctgat ctggacgctg
     3721 ctgcagcgcc tgataacccg gcagtcgctc cagcagcctt tggccttcgg gccctggctg
     3781 gcgctggcgg gcggcggcat ctttttgtgg agccaaattt acccccccac tccccggtgg
     3841 cgctgcgctg accgggctac gggttccccg ccgtctgcgg gccggtagcc cggacaggtg
     3901 cgccagcacc ggctccggga agccgtaccc accgaggctt cgcatactcc cccccggtgg
     3961 cggctgacgc cttaccgggg ctacgggttc cccgccgtct gcgggccggt agcccggaca
     4021 ggtgcgccag caccggctcc gggaagccgt acccaccgag gcttcgcata ctcccccccg
     4081 gtggcggctg acgccttacc ggggctacgg gttccccgcc gtctgcggac cggtagcccg
     4141 gccaggtgcg ccagcaccgc ctccgggaag ccgttcctac cgaggcttcg catactcccc
     4201 cccggtggcg gctgacgcct taccggggct acgggttccc cgccgtctgc ggaccggtag
     4261 cccggacagg tgcgccagca ccgcctccgg gaaaataacc ggttacgaat attttttggt
     4321 gcgccgggtc ggggcggtat tggccggatc gtccggccag acgtgcttcg gatagcgccc
     4381 tttcatctct ttttgcacct cgcggtagct cccctgccag aatgcgccta aatcgcgggt
     4441 gatctgcagc gggcgctgcg ccggcgacag tagctccagc accagcggca cccgcccctg
     4501 ggcgatagtc ggggtcgtcg cctcgccaaa catctcctgc atccgcaccg ccagcgccgg
     4561 agggttatca tcgtgataac gaatcgcgat gcggctaccg gtcggcaccg tatagtgcgt
     4621 gggtagctcg ctgtccagct tctggcgcag cggccacggc agccagtttt gcagcgcctg
     4681 acgcacatcc agcgccttca gcgcgcgcag agaatggacg cccgtcatct gcggtaacag
     4741 ccactgctca agcgatgcca gcagcgatgc ctcatcaacg gctggccacg cctcctccgg
     4801 cagccactgc gccgcgcagt gcaggcgcaa acgcagctgc tccgcctccg gcgtccagtt
     4861 gagcacgctc agcccctttt cgcggatgcc gttgagcatc gcctgatgca gctcctcttc
     4921 cgacggcttc gccagcggtt gagttttaat caccagccgt ccgatacagg tacgccgcca
     4981 ggccttcagc gtgcctaacg cctcatccca ctcgacgata tcggagcgct ggaccagcgc
     5041 cggacaccgc tcaatcagcg cgttaatctc taccggcagc gc
//