From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human myeloperoxidase mRNA, complete cds.
Date: 5 Nov 92 23:10:29 GMT
Distribution: bionet
Checksum: 15003 8

LOCUS       HUMMPO       3215 bp ss-mRNA            PRI       15-JUN-1989
DEFINITION  Human myeloperoxidase mRNA, complete cds.
ACCESSION   M19507 M17709
KEYWORDS    myeloperoxidase.
SOURCE      Human promyelocytic leukemia cell line HL-60, cDNA to mRNA, clone
            pMP1 [1], clone lambda-MP-H17 [3],[2].
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1939 to 3208)
  AUTHORS   Yamada,M., Hur,S.-J., Hashinaka,K., Tsuneoka,K., Saeki,T.,
            Nishio,C., Sakiyama,F. and Tsunasawa,S.
  TITLE     Isolation and characterization of a cDNA coding for human
            myeloperoxidase
  JOURNAL   Arch. Biochem. Biophys. 255, 147-155 (1987)
  STANDARD  full automatic
REFERENCE   2  (bases 1 to 3215)
  AUTHORS   Yamada,M.
  JOURNAL   Unpublished (1988) Osaka Univ., Suita, Osaka Japan 565
  STANDARD  full automatic
REFERENCE   3  (bases 58 to 3215)
  AUTHORS   Hashinaka,K., Nishio,C., Hur,S.-J., Sakiyama,F., Tsunasawa,S. and
            Yamada,M.
  TITLE     Multiple species of myeloperoxidase messenger RNAs produced by
            alternative splicing and differential polyadenylation
  JOURNAL   Biochemistry 27, 5906-5914 (1988)
  STANDARD  full automatic
COMMENT     Draft entry and printed copy of sequence for [1] kindly provided by
            M.Yamada, 23-NOV-1987 and for [3] 11-MAY-1988.
FEATURES             Location/Qualifiers
     mRNA            <1..2590
                     /gene="MPO"
     mRNA            <1..2594
                     /gene="MPO"
     mRNA            <1..2597
                     /gene="MPO"
     mRNA            <1..3208
                     /gene="MPO"
     mRNA            <1..3215
                     /gene="MPO"
     mat_peptide     670..993
                     /product="myeloperoxidase light chain"
                     /gene="MPO"
                     /codon_start=1
     mat_peptide     1012..2409
                     /product="myeloperoxidase heavy chain"
                     /gene="MPO"
                     /codon_start=1
     CDS             178..2415
                     /EC_number="1.11.1.7"
                     /product="myeloperoxidase"
                     /codon_start=1
                     /translation="MGVPFFSSLRCMVDLGPCWAGGLTAEMKLLLALAGLLAILATPQ
                     PSEGAAPAVLGEVDTSLVLSSMEEAKQLVDKAYKERRESIKQRLRSGSASPMELLSYF
                     KQPVAATRTAVRAADYLHVALDLLERKLRSLWRRPFNVTDVLTPAQLNVLSKSSGCAY
                     QDVGVTCPEQDKYRTITGMCNNRRSPTLGASNRAFVRWLPAEYEDGFSLPYGWTPGVK
                     RNGFPVALARAVSNEIVRFPTDQLTPDQERSLMFMQWGQLLDHDLDFTPEPAARASFV
                     TGVNCETSCVQQPPCFPLKIPPNDPRIKNQADCIPFFRSCPACPGSNITIRNQINALT
                     SFVDASMVYGSEEPLARNLRNMSNQLGLLAVNQRFQDNGRALLPFDNLHDDPCLLTNR
                     SARIPCFLAGDTRSSEMPELTSMHTLLLREHNRLATELKSLNPRWDGERLYQEARKIV
                     GAMVQIITYRDYLPLVLGPTAMRKYLPTYRSYNDSVDPRIANVFTNAFRYGHTLIQPF
                     MFRLDNRYQPMEPNPRVPLSRVFFASWRVVLEGGIDPILRGLMATPAKLNRQNQIAVD
                     EIRERLFEQVMRIGLDLPALNMQRSRDHGLPGYNAWRRFCGLPQPETVGQLGTVLRNL
                     KLARKLMEQYGTPNNIDIWMGGVSEPLKRKGRVGPLLACIIGTQFRKLRDGDRFWWEN
                     EGVFSMQQRQALAQISLPRIICDNTGITTVSKNNIFMSNSYPRDFVNCSTLPALNLAS
                     WREAS"
BASE COUNT      652 a    949 c    933 g    681 t
ORIGIN      54 bp upstream of PvuII site; chromosome 17q21-q23.
        1 gacaatatca ggtgagctgt ggaggtgggg tccttggaag ctggatgaca gcagctggca
       61 aggggataag agagcagtga gcccctccct caaggaggtc tggctttatc catagacagg
      121 gccctctgag gtggggctga ggtacaaagg gggattgagc agcccaggag aagagagatg
      181 ggggttccct tcttctcttc tctcagatgc atggtggact taggaccttg ctgggctggg
      241 ggtctcactg cagagatgaa gctgcttctg gccctagcag ggctcctggc cattctggcc
      301 acgccccagc cctctgaagg tgctgctcca gctgtcctgg gggaggtgga cacctcgttg
      361 gtgctgagct ccatggagga ggccaagcag ctggtggaca aggcctacaa ggagcggcgg
      421 gaaagcatca agcagcggct tcgcagcggc tcagccagcc ccatggaact cctatcctac
      481 ttcaagcagc cggtggcagc caccaggacg gcggtgaggg ccgctgacta cctgcacgtg
      541 gctctagacc tgctggagag gaagctgcgg tccctgtggc gaaggccatt caatgtcact
      601 gatgtgctga cgcccgccca gctgaatgtg ttgtccaagt caagcggctg cgcctaccag
      661 gacgtggggg tgacttgccc ggagcaggac aaataccgca ccatcaccgg gatgtgcaac
      721 aacagacgca gccccacgct gggggcctcc aaccgtgcct ttgtgcgctg gctgccggcg
      781 gagtatgagg acggcttctc tcttccctac ggctggacgc ccggggtcaa gcgcaacggc
      841 ttcccggtgg ctctggctcg cgcggtctcc aacgagatcg tgcgcttccc cactgatcag
      901 ctgactccgg accaggagcg ctcactcatg ttcatgcaat ggggccagct gttggaccac
      961 gacctcgact tcacccctga gccggccgcc cgggcctcct tcgtcactgg cgtcaactgc
     1021 gagaccagct gcgttcagca gccgccctgc ttcccgctca agatcccgcc caatgacccc
     1081 cgcatcaaga accaagccga ctgcatcccg ttcttccgct cctgcccggc ttgccccggg
     1141 agcaacatca ccatccgcaa ccagatcaac gcgctcactt ccttcgtgga cgccagcatg
     1201 gtgtacggca gcgaggagcc cctggccagg aacctgcgca acatgtccaa ccagctgggg
     1261 ctgctggccg tcaaccagcg cttccaagac aacggccggg ccctgctgcc ctttgacaac
     1321 ctgcacgatg acccctgtct cctcaccaac cgctcagcgc gcatcccctg cttcctggca
     1381 ggggacaccc gttccagtga gatgcccgag ctcacctcca tgcacaccct cttacttcgg
     1441 gagcacaacc ggctggccac agagctcaag agcctgaacc ctaggtggga tggggagagg
     1501 ctctaccagg aagcccggaa gatcgtgggg gccatggtcc agatcatcac ttaccgggac
     1561 tacctgcccc tggtgctggg gccaacggcc atgaggaagt acctgcccac gtaccgttcc
     1621 tacaatgact cagtggaccc acgcatcgcc aacgtcttca ccaatgcctt ccgctacggc
     1681 cacaccctca tccaaccctt catgttccgc ctggacaatc ggtaccagcc catggaaccc
     1741 aacccccgtg tccccctcag cagggtcttt tttgcctcct ggagggtcgt gctggaaggt
     1801 ggcattgacc ccatcctccg gggcctcatg gccacccctg ccaagctgaa tcgtcagaac
     1861 caaattgcag tggatgagat ccgggagcga ttgtttgagc aggtcatgag gattgggctg
     1921 gacctgcctg ctctgaacat gcagcgcagc agggaccacg gcctcccagg atacaatgcc
     1981 tggaggcgct tctgtgggct cccgcagcct gaaactgtgg gccagctggg cacggtgctg
     2041 aggaacctga aattggcgag gaaactgatg gagcagtatg gcacgcccaa caacatcgac
     2101 atctggatgg gcggcgtgtc cgagcctctg aagcgcaaag gccgcgtggg cccactcctc
     2161 gcctgcatca tcggtaccca gttcaggaag ctccgggatg gtgatcggtt ttggtgggag
     2221 aacgagggtg tgttcagcat gcagcagcga caggccctgg cccagatctc attgccccgg
     2281 atcatctgcg acaacacagg catcaccacc gtgtctaaga acaacatctt catgtccaac
     2341 tcatatcccc gggactttgt caactgcagt acacttcctg cattgaacct ggcttcctgg
     2401 agggaagcct cctagaggcc aggtaagggg gtgcagcagt gaggggtata tctgggctgg
     2461 ccagttggaa ccacggagat ctccttgccc tagatgagcc cagccctgtt ctgggtgcag
     2521 ctgagaaaat gagtgactag acgttcattt gtgtgctcat gtatgtgcga agtatataaa
     2581 ttggcttttc atgcgtgtgt gttgtctgaa catggggagt gtttcatggg ttatgtgtat
     2641 gtgccattta tgtgagtgtg tgtttgtgct gatgagaata ctgagtatgt ggaaggcagc
     2701 agagcggact ggtgaggagc acagctcagg aactagactg cctgggttcc aatcctggct
     2761 ctgtggcttg ctagctatgt gaccttgagc aaattaccct ccttaaacaa gagttttctt
     2821 ccttgtaaat tacatctgtc atggtttctt ggagggccca cttgtatcct ctggttcttc
     2881 atttattgag cacctactac atgcaaggca ctgtactagg cgtgagaagc atatagaggc
     2941 aagaaagaga taccaagatg ccatctgtgt cctggttagc agagctggac cagtggtgcc
     3001 ttggagggat aagccagctg cagctgggct gtgtggttga cttatgggcc cagccagcca
     3061 ggctcaggcc atggctcccc tttttcttcc tcaccctgat ttcttgctta ttcactgaag
     3121 ttctcctgaa gaggaactgg gcctgttgcc ctttctgtac catttatttg ctcccaatgt
     3181 ttatgataat aaaggcaccg ctgatgggga cctcc
//