From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Rhizobium meliloti plasmid nfe locus ORF 7 and ORF 8, complete cds;
Date: 29 Jul 92 10:03:44 GMT
Distribution: bionet
Checksum: 36039     8

LOCUS       RHMPLNFE     3345 bp ds-DNA             BCT       29-JUL-1992
DEFINITION  Rhizobium meliloti plasmid nfe locus ORF 7 and ORF 8, complete cds;
            Nfe1 and Nfe2 genes, complete cds.
ACCESSION   X66124
KEYWORDS    Nfe1 gene; Nfe2 gene.
SOURCE      Rhizobium meliloti DNA.
  ORGANISM  Rhizobium meliloti
            Prokaryotae; Gracilicutes; Scotobacteria; Rhizobiaceae.
REFERENCE   1  (bases 1 to 3345)
  AUTHORS   Soto,M.J., Zorzano,A., Mercado,J., Lepek,V., Olivares,J. and
            Toro,N.
  TITLE     Nucleotide sequence and characterization of Rhizobium meliloti
            nodulation competitiveness locus nfe
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
COMMENT     *source: strain=GR4;
            From EMBL    entry RMPLNFE;  dated 12-JUN-1992.
FEATURES             Location/Qualifiers
     CDS             complement(<1..622)
                     /note="ORF8"
                     /codon_start=1
                     /translation="MRPSRPSSGKNDLFRDRLDAIINPRQALVRLAGVIPWARFDEAF
                     GGFYRPVGRPANATRLMVGLHYLKHVHDLSDEEVVERWVENPYWQFFCGFEFFQHEAP
                     IDASTMTPWRKRIGPQGLEELLKASVEAALQTGTARPESLERVNVDTTVQPKAIAHPT
                     DSRLYLKALQMLVRHAKKHGIELRQSYTRLAKRAAVRARRYAHARQF"
     CDS             complement(876..1574)
                     /note="ORF7"
                     /codon_start=1
                     /translation="MNAHNMRGPPGDLAKVVPGSRSGWNEGSRCRQADKGDGQCRNGG
                     RDASEHVEARALFLNSNISSKARFSLRTPRVVPSSRCRRRRADAEARRRTAHARHDTN
                     LRTGERCPNESPLLAAILADATNLGLSRRAAASQGVTATNSPGRRMPLSGSFHPIAHK
                     QLVAAAYASNAVSMDGDIQQDNLNGFLTSEPAARGCGQGRRLASSRPCMMASFHRNQS
                     FARAHRRPTPTRPD"
     misc_binding    1472..1487
                     /bound_moiety="unspecified"
                     /standard_name="NifA-binding motif"
                     /gene="Nfe1"
     misc_feature    1520..1536
                     /note="nif consensus"
                     /gene="Nfe1"
     CDS             1606..2031
                     /gene="Nfe1"
                     /codon_start=1
                     /translation="MAALRQVAFYGKGGIGNSKRKPELVTASIEDRSAGSPSKNKMHF
                     QSRMNVAASMRGGDGLPPSVRCRLHLEDGTTRGERKERTRLAATFQSVSEERPRLHMP
                     RRVTTTHTTLFVTFVGFTTPIVPSTVWSISRSNHLKQSN"
     misc_binding    1953..1968
                     /bound_moiety="unspecified"
                     /standard_name="NifA-binding motif"
                     /gene="Nfe2"
     misc_feature    2055..2071
                     /note="nif consensus"
                     /gene="Nfe2"
     CDS             2242..3177
                     /gene="Nfe2"
                     /codon_start=1
                     /translation="MTDLIGFYSDHKIAAPLAIAQRSAKFKPFAAEIARRGILQIATC
                     ARLEFYGEKSVLEDIDGKPFFGFSYGRVEGAISIAERLATIAAGVHSQILGEGFISGQ
                     LMQAIESGDPNLSIFKVARVAVDLGSAARKRQEFFAEFDYVQIVEDIIADRFSDKAPL
                     DRIYIIGAGMLGQELIRGNVGERFRSTVVITRNPKKLRKRLNTGPKTNVTFMRPSEVG
                     RTREPGSMVVIATTDVDNKYKAILQETLLRLGPRIILDLSSIPVLTREAIGELDYVNM
                     YGDEFLRFIEQNNQRLAPKLPLVVSDIKAALGAAV"
BASE COUNT      721 a    988 c    958 g    678 t
ORIGIN
        1 ggaattgccg ggcatgggca taacggcgcg cgcgcaccgc cgctcgtttc gccagacgcg
       61 tgtagctctg cctcagctcg atgccatgct tcttggcgtg acggaccagc atctgcagag
      121 ccttcagata cagcctgctg tcggtcggat gagcgatggc tttcggctgc accgtcgtgt
      181 cgacattgac ccgctccaaa gattccggcc tggccgttcc cgtctgaagc gccgcctcga
      241 cgctggcctt cagcaattcc tccagcccct gcggcccgat ccgcttgcgc cacggcgtca
      301 tcgtgctcgc gtcgatcggc gcttcatgct ggaagaactc aaagccgcag aaaaactgcc
      361 aatacggatt ctccacccag cgctcgacca cctcctcatc cgacaggtcg tggacatgct
      421 tcaggtaatg cagcccgacc atcagccgcg tcgcgttggc gggccggcca accggccgat
      481 agaacccacc gaacgcctcg tcaaatcgtg cccatgggat cacaccggcc aatcgcacca
      541 gcgcctggcg cgggttgatg atcgcgtcca gacggtcgcg aaaaagatca ttcttaccgg
      601 aagagggacg acttggccgc ataccaaaaa caccagaaat cagctacatc accgacgatt
      661 tccggcagtt tccaatactt caaaaaagat gggaatccct taatcgaagg cgttgagagg
      721 tttttcagcg ccgaccaagt atccggcaac tccttcctgg cgatgaaggt cacgttcatc
      781 aacgaaatgg cggacctctg cgaaaaggtc ggggcggagg tgcagaaggt cgccaaggca
      841 tggcctcggt agtggctcag ggcccgcgaa aggatttaat cgggccgggt gggggtgggt
      901 ctgcggtgcg ctcgagcgaa cgactgattg cggtggaagc tcgccatcat gcaaggcctg
      961 gatgatgcta gcctacgtcc ttgtccgcag ccgcgagccg ctggttcaga cgtaaggaat
     1021 ccgttcaggt tgtcctgctg aatgtctcca tccatactga cggcgtttga agcgtacgct
     1081 gcagcgacca gttgtttgtg agcgatgggg tggaagctcc cactcaacgg catcctgcgt
     1141 ccaggtgagt tggtggcggt caccccctgg ctggcggcag ccctccggga gagcccaaga
     1201 ttggtggcgt cggccagaat ggcggcgaga aggggacttt cgttggggca gcgttcgccg
     1261 gtgcgcaggt ttgtgtcatg tcgagcatgc gcggtgcggc gacgggcttc cgcgtccgca
     1321 cgccgccgtc tgcacctcga ggatggcacc acgcgcggcg tgcgcaaaga gaagcgggcc
     1381 ttgctgctaa tgttagagtt taggaagagc gcccgtgcct ccacatgctc ggacgcgtca
     1441 cgaccaccgt tacgacattg tccgtcacct ttgtcggctt gacgacaccg actaccttcg
     1501 ttccagccac tcctgctgcc cggcacgact tttgcaagat cacccggtgg gccgcgcatg
     1561 ttgtgcgcat tcattgtccc gaacaaaaac aacaaaggaa gcaagatggc tgctctgcgt
     1621 caggtcgcgt tctacggtaa gggcggtatc ggcaactcca agcggaagcc cgagctggta
     1681 accgcgtcca tcgaagacag gagcgctggc tccccgtcga aaaacaaaat gcacttccag
     1741 agccgcatga atgtcgcggc gagcatgcgc ggcggcgacg ggcttccgcc ttccgtccga
     1801 tgccgtctgc acttagagga cggcaccacg cgcggcgagc gcaaggagag gacgcgcctt
     1861 gccgctactt tccaaagtgt ttcggaagag cgcccccgcc tccacatgcc ccgacgcgtg
     1921 acgaccaccc atacgacatt gttcgtcacc tttgtcggct ttacgacacc aatcgttcct
     1981 tcaaccgtgt ggtcaatttc ccgatctaac catctgaaac aaagcaatta gcattgcttc
     2041 agtcactctg cggcctggca tgacttttgc acgattatcc ctgggcgcgc atgctgttgc
     2101 gcattcatgt gtccgaacaa ccgaaatagg agatccaagg gtcaaaatac gccacgctca
     2161 cgcactcgag gagaaagcca agttcgggcg cgttctgcgt caacttccat atgagcttca
     2221 cgtagtctcg gagatcaagc gatgaccgac ctcatcggat tttacagtga tcacaagata
     2281 gccgcgcctc tggcaatcgc gcagcgatcg gcgaagttta aacccttcgc cgctgagatc
     2341 gcgagacgtg gaattctgca gatcgcgacc tgcgcgcgcc ttgagttcta tggcgaaaaa
     2401 tctgtgctcg aagatatcga cgggaagcct ttttttggct tttcatatgg ccgcgtcgaa
     2461 ggtgccatct caattgccga acgtctcgcc acgattgcag ccggcgttca ctctcagatt
     2521 ctcggtgagg gcttcatttc cggccagctc atgcaggcga tcgaatctgg tgatccaaac
     2581 ctttcgatat tcaaggtggc ccgggtagca gttgatctgg gcagcgccgc ccgcaagcga
     2641 caggagtttt tcgccgagtt cgactacgta cagatcgttg aagatataat agcggatcga
     2701 ttttcggaca aagcgccact cgaccgcatt tacatcatcg gggcgggcat gctcggacag
     2761 gagctgatca ggggtaatgt cggagagcgt tttcgttcga cggtggtcat tacgcggaat
     2821 cccaagaagc tccgaaagcg cctcaacacc gggcctaaga caaacgtcac gttcatgcgc
     2881 ccgtcagagg tcgggcgcac acgggagccg gggtcaatgg tcgttatagc aaccaccgat
     2941 gtcgataata agtacaaggc cattctccag gagacccttc tgcgattggg ccctcgcatc
     3001 atactggatc tgtcgtcaat tccggtgctg acgcgcgaag cgatcggaga gctcgactat
     3061 gtcaacatgt atggcgacga atttctccgg ttcatcgaac aaaacaacca gcggctagct
     3121 ccgaaactac cgctcgtggt ttccgacata aaagcggcac tcggagcggc agtctagggg
     3181 catagccatc aggctgtacg caccgccttc agaggatcat ttaaaacgct taaacccgtt
     3241 cgtgctcgga cattcccacg aggccgagct gttccaaaag caataggaag gaggtaaaaa
     3301 tcatgatcgc tcctgtgaga tggcttagtc ggaaagacgt tgcac
//