From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Escherichia coli gltBDF operon gltF gene (involved in
Date: 6 Nov 92 18:06:05 GMT
Distribution: bionet
Checksum: 64255 6

LOCUS       ECOGLTF      2315 bp ds-DNA             BCT       28-OCT-1992
DEFINITION  Escherichia coli gltBDF operon gltF gene (involved in
            nitrogen-regulated gene expression), complete cds.
ACCESSION   M74162
KEYWORDS    gltF gene; regulatory protein.
SOURCE      Escherichia coli (strain K-12) DNA.
  ORGANISM  Escherichia coli
            Prokaryotae; Gram-negative facultatively anaerobic rods;
            Enterobacteriaceae.
REFERENCE   1  (bases 1 to 2315)
  AUTHORS   Flores,N., Valle,F., Covarrubias,A., Bolivar,F. and Castano,I.
  TITLE     gltF a member of the gltBDF operon of Escherichia coli is involved
            in nitrogen-regulated gene expression
  JOURNAL   Mol. Microbiol. 6, 2733-2741 (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     -35_signal      470..475
                     /gene="gltF"
     -10_signal      492..497
                     /gene="gltF"
     RBS             536..539
                     /gene="gltF"
     sig_peptide     549..623
                     /gene="gltF"
                     /codon_start=1
     mat_peptide     624..1310
                     /gene="gltF"
                     /codon_start=1
     protein_bind    736..759
                     /bound_moiety="ATP"
     -35_signal      1433..1438
     -10_signal      1457..1462
     RBS             1469..1472
     CDS             549..1313
                     /function="involved in nitrogen-regulated gene expression"
                     /note="gltF"
                     /gene="gltF"
                     /codon_start=1
                     /translation="MFFKKNLTTAAICAALSVAAFSAMATDSTDTELTIIGEYTPGAC
                     TPVVTGGGIVDYGKHHNSALNPTGKSNKLVQLGRKNSTLNITCTAPTLIAVTSKDNRQ
                     STIVALNDTSYIEKAYDTLVDMKGTKNAFGLGSAPNGQKIGAASIGIDRSNGGIHAAD
                     DTGEIPVDLIQTDHWSAATPTWKASSNGAFCSLTSCSAIERGYSVAKTGELTPVAITA
                     VTFPLLIDAAVNDNTILGSDETIKLDGNVTISVQYL"
     CDS             1485..2135
                     /note="ORF"
                     /codon_start=1
                     /translation="MLRHITFTVFITTSMNTLATGMVLKHQYCWLMKSVVKLVLILRI
                     QMIILHCFTPPLLILPESNKSIRLIPTQPVIRVEAGQVQQVRFLLQATVPLQSEELKR
                     VTFEGIPPKDDKSSRVTVSIRQDLPVLIHPASLPEERETWKFLEWRKNGDQIEISNPS
                     NYVVRMTLQFKTLPSGKTGAINKTYFLPHTSTTTALTNATDTKGRVLPSQPIWLSR"
BASE COUNT      716 a    493 c    427 g    679 t
ORIGIN      
        1 gaattcgcca gactgtgtct gggcggaatc aaattactga cgaagtgcgc gttgttttct
       61 ggcaaatgcg gcatgagcgt ttgacctttc ctataatagg gataattttc aataaattgt
      121 gctttgttca ccaagtctga tgagtgaagc acatcaagca atttctcatt tacaatccgt
      181 ctcaattact tgttaacacc tctgacaata ccgattctgc aagctattca gtcttttcat
      241 tccatgtaaa ttgtctcgat attaatatac aaaatatgaa tataaaaaac caatatatta
      301 tccttaatta tctatatatt ttcctatttt tcagcgcaaa cattcatgta atgaataatt
      361 atcaccacaa accacctcac cagcacctta ttattcacat gatcgtaact ataaaattca
      421 ttaaataaat acatcccagc aagttggcta tttctgaacg agttatttat ttacattcac
      481 aaacagatat atatactgct tcagcttttt attttaaaaa ataaatttgt tattaaggat
      541 atgttcatat gtttttcaaa aagaacctca caacagctgc tatttgtgca gcactttctg
      601 ttgcagcatt cagtgccatg gcaacagatt caactgatac agaattaact atcatcggcg
      661 agtatacccc tggtgcatgt accccagttg ttacgggcgg cggcattgtt gactatggga
      721 aacatcataa cagtgcgctg aatccgaccg gtaaaagtaa taaactggtt caactgggcc
      781 gcaaaaacag cacattaaat ataacctgca cagcaccaac gctaattgct gtaacctcca
      841 aggataaccg tcagtcgacc attgtagcgt taaacgacac atcctatatt gaaaaagctt
      901 atgatactct cgtagatatg aagggaacga aaaacgcctt tggtttaggt tctgcgccta
      961 atggacaaaa aattggcgct gcatccattg gtattgacag gtctaatggt gggattcacg
     1021 cggcggacga tacaggtgaa attcctgtcg acctgattca aactgaccac tggtcagcag
     1081 cgacccccac atggaaagcc tcatctaatg gcgcattctg ctcgctgaca agttgctcag
     1141 caatcgagcg cggttattct gttgcaaaaa caggggaact gacacctgtt gctattacag
     1201 cggtcacctt cccgttactg attgatgctg cggtaaatga caatactatt cttggttctg
     1261 atgaaacgat caaactggat ggtaacgtca ctatcagcgt tcagtattta taagaacttg
     1321 taatacagtt tttattttta cgcattaacc agttagctat aactcggaca gattatgccc
     1381 tatgacatgg tctgtcttaa tcaatctcat cgcaaacagt gcataacata tattaacaat
     1441 atgtttctgc gttatttatc attattacag gagttttaat ttaaatgctc agacacatta
     1501 cattcactgt atttataaca acatcaatga ataccttagc cacagggatg gttctgaaac
     1561 atcagtattg ctggttgatg aaaagcgtgg tgaagctagt attaatatta agaatacaga
     1621 tgatcatcct tcattgcttt acaccaccat tgttgatttt gcctgaaagc aataaatcaa
     1681 ttcgtttaat cccgactcag cccgttattc gtgttgaagc cggtcaagta caacaggttc
     1741 gatttttact tcaggcgact gttcctctgc aatcagaaga gctgaaacgc gtaacgttcg
     1801 aaggtattcc accaaaagat gacaagagca gcagagttac tgtttcaatt cgtcaggatc
     1861 tacctgtact gatccacccg gcttccctgc cggaagagcg agagacctgg aaattcctgg
     1921 aatggcgtaa aaatggcgac caaattgaga ttagcaaccc cagtaattat gtcgttcgta
     1981 tgaccttaca atttaaaacg ctcccttcag gaaaaacggg ggctataaat aagacctatt
     2041 ttctaccaca caccagtaca actactgcat taacaaacgc tacggataca aaaggtcgag
     2101 ttttacccag ccagccgata tggttatcgc ggtaataaat atgtcactga cttaaaataa
     2161 ctttgcctgg agcgacaagg atgttaaaaa aaacgttact ggcctacacc attggttttg
     2221 ccttttctcc cccagccaat gcagatggta tagagattgc cgctgttgat tttgatcggg
     2281 aaacattaaa tcactaggtg tagatcctaa tatat
//