From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: P.boryanum frxC gene for dinitrogenase reductase-like protein
Date: 13 Aug 92 10:01:04 GMT
Distribution: bionet
Checksum: 17000     5

LOCUS       PEEFRXC      1521 bp ds-DNA             BCT       13-AUG-1992
DEFINITION  P.boryanum frxC gene for dinitrogenase reductase-like protein
            involved in the light independent reduction of protochlorophyllide.
ACCESSION   D00665
KEYWORDS    dinitrogenase reductase-like protein; frxC.
SOURCE      P.boryanum (strain M101) DNA.
  ORGANISM  Plectonema boryanum
            Prokaryotae; Gracilicutes; Oxyphotobacteria; Cyanobacteria;
            Nostocales; Scytonemataceae.
REFERENCE   1  (bases 1 to 1521)
  AUTHORS   Fujita,Y., Takahashi,Y., Shonai,F., Ogura,Y. and Matsubara,H.
  TITLE     Cloning, nucleotide sequences and differential expression of the
            nifH and nifH-like (frxC) genes from the filamentous
            nitrogen-fixing cyanobacterium Plectonema boryanum
  JOURNAL   Plant Cell Physiol. 32, 1093-1106 (1991)
  STANDARD  full staff_review
COMMENT     These data kindly submitted in computer readable form by:
            Yuichi Fujita
            Department of Biology
            Faculty of Science, Osaka Univ.
            1-1 Machikaneyama
            Toyonaka, Osaka 560
            Japan
            Phone:  06-844-1151 x4296
            Fax:    06-855-8139
FEATURES             Location/Qualifiers
     -35_signal      4..9
                     /note="put. promoter 1 (-35)"
     -10_signal      26..31
                     /note="put. promoter 1 (-10)"
     -35_signal      81..86
                     /note="put. promoter 2 (-35)"
     -10_signal      105..110
                     /note="put. promoter 2 (-10)"
     RBS             196..200
     CDS             210..1070
                     /note="nitrogenase reductase-like protein (gtg start
                     codon)"
                     /codon_start=1
                     /translation="MKLAVYGKGGIGKSTTSCNISVALAKRGKKVLQIGCDPKHDSTF
                     TLTGFLIPTIIDTLQEKDYHYEDVWAEDVIYKGYGGVDCVEAGGPPAGAGCGGYVVGE
                     TVKLLKELNAFDEYDVILFDVLGDVVCGGFAAPLNYADYCMIVTDNGFDALFAANRIA
                     ASVREKARTHPLRLAGLIGNRTAKRDLIEKYVDAVPMPILEVLPLIEDIRVSRVKGKT
                     LFEMAESDPSLNYVCDYYLNIADQILANPEGVVPKDAADRDLFSLLSDFYLNPQQPKT
                     AEEELDLMMV"
     CDS             1158..>1521
                     /note="URF1 (gtg start codon)"
                     /codon_start=1
                     /translation="MNIDQLRRSLKDRWLDYYEANRSWISRLSIWVSCEGKRRPSSSF
                     ILGVLSTEEPRLTEMLPLIVDLSNHPDRIVVALGLNFDPEQELNHKQLKAETPKYLPA
                     SSATEAPQKPRRPAEVDEA"
BASE COUNT      417 a    314 c    372 g    418 t
ORIGIN
        1 gaattcaaga atttgaaaat tgctaaataa ttgcttaatt caccttagta agatttcttg
       61 gagttctgat aaatatattc ttcaactgaa ttgtcttagc gatgtaactt ctttttgcga
      121 ggactaggca gaaacggtct tatcaacaac aatgcaaaca gaacagattt gtactcaccc
      181 cttcagagac gacttaggag aaccttagag tgaaactcgc agtgtatggc aaaggcggca
      241 tcggcaaatc gacgacaagc tgcaacatct cagtcgcatt ggcaaaacgc ggcaaaaagg
      301 ttttacaaat cggttgcgat cccaagcacg atagtacgtt taccttgact ggcttcttga
      361 ttccaaccat tattgatacg cttcaggaaa aggactatca ctatgaggat gtctgggctg
      421 aagatgttat ctacaaaggc tatggcggtg tggactgtgt agaggcaggt gggcctccag
      481 caggagcagg ctgcggtggt tatgtagtcg gtgaaacggt caaactgctc aaagaactga
      541 acgcatttga cgaatatgat gtgattctgt ttgatgtctt aggagacgtg gtttgtggcg
      601 gattcgctgc tccattgaac tatgcagact attgcatgat cgtgacagat aacggatttg
      661 atgcgttgtt tgcagcaaat cggattgcgg cttcggtcag agagaaagcg cggacacatc
      721 cactacgatt agcaggactg attggtaatc gaaccgccaa gcgggatttg attgaaaagt
      781 atgtcgatgc agttccgatg ccgatcttgg aagtgttgcc tttgattgaa gacattcgcg
      841 tttcgcgagt caaaggaaaa accttgtttg aaatggcgga gtctgatccc tcattgaact
      901 atgtgtgtga ttactatctg aacattgctg atcagatttt ggcaaatcct gaaggagtcg
      961 ttccgaaaga tgctgccgat cgcgatttat tctcgttgct gtctgacttc tatctcaatc
     1021 cacaacagcc aaaaactgcc gaggaagaat tagatttgat gatggtctaa agaaagaatg
     1081 aaggggaaag gatgaaggct acgaattgcg atatgtgatc tttagtgcca actttgtcct
     1141 tttactttgt ttgacttgtg aacattgatc aattacgtcg atcgctcaaa gaccgctggc
     1201 tcgactatta cgaagcaaac cgttcttgga tttctcggtt gtcgatctgg gtcagttgcg
     1261 aagggaaacg gcgaccgtct tctagcttta ttctgggtgt tctctcaacc gaagaacccc
     1321 gtctgaccga aatgctgccg ctcattgtgg acttgagcaa tcatcccgat cgcattgttg
     1381 ttgcactggg actgaacttt gatcccgaac aagaactgaa tcacaaacaa ctcaaagctg
     1441 aaactcccaa gtatcttcct gcgagttctg caaccgaagc gcctcagaaa cctcgtcgtc
     1501 cggctgaagt tgatgaagct t
//