From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: toluene-4-monooxygenase gene cluster: tmoF=putative NADH:ferredoxin
Date: 27 Jan 93 05:56:08 GMT
Distribution: bionet
Checksum: 26708 4

LOCUS       S118027      1180 bp    DNA             BCT       21-JAN-1993
DEFINITION  toluene-4-monooxygenase gene cluster: tmoF=putative NADH:ferredoxin
            oxidoreductase component [Pseudomonas mendocina, KR1, Genomic, 1180
            nt]
ACCESSION   S49343 M95045
KEYWORDS    .
SOURCE      Pseudomonas mendocina KR1
  ORGANISM  Pseudomonas mendocina
            Unclassified.
REFERENCE   1  (bases 1 to 1180)
  AUTHORS   Yen,K.M. and Karl,M.R.
  TITLE     Identification of a new gene, tmoF, in the Pseudomonas mendocina
            KR1 gene cluster encoding toluene-4-monooxygenase.
  JOURNAL   J. Bacteriol. 174, 7253-7261 (1992)
  STANDARD  full automatic
COMMENT     This entry [NCBI gibbsq 118027] was created by the journal scanning
            component of NCBI/GenBank at the National Library of Medicine.
            This sequence comes from Fig. 4.  The authors begin their numbering
            at 3550.
            Region: toluene-4-monooxygenase gene cluster.  
FEATURES             Location/Qualifiers
     CDS             49..1029
                     /note="putative NADH:ferredoxin oxidoreductase component;
                     For the protein sequence (NCBI gibbsq 118029):  Method:
                     conceptual translation supplied by author.  This sequence
                     comes from Fig. 4."
                     /gene="tmoF"
                     /product="TmoF"
                     /codon_start=1
                     /translation="MFNIQSDDLLHHFEADSNDTLLSAALRAELVFPYECNSGGCGAC
                     KIELLEGEVSNLWPDAPGLAARELRKNRFLACQCKPLSDLKIKVINRAEGRASHPPKR
                     FSTRVVSKRFLSDEMFELRLEAEQKVVFSPGQYFMVDVPELGTRAYSAANPVDGNTLT
                     LIVKAVPNGKVSCALANETIETLQLDGPYGLSVLKTADETQSVFIAGGSGIAPMVSMV
                     NTLIAQGYEKPITVFYGSRLEAELEAAETLFGWKENLKLINVSSSVVGNSEKKYPTGY
                     VHEIIPEYMEGLLGAEFYLCGPPQMINSVQKLLMIENKVPFEAIHFDRFF"
BASE COUNT      300 a    256 c    311 g    313 t
ORIGIN      
        1 ggtcttcccc ttcggcattg ctgaaggggc tttttagaga cgttatctat gttcaatatt
       61 caatcggatg atctcctgca ccattttgag gcggatagta atgacactct acttagtgct
      121 gctctacgtg ctgaattggt atttccatat gagtgtaact caggagggtg cggcgcatgt
      181 aagatcgagc tgcttgaggg agaggtctct aacctatggc ctgatgcacc aggattagcc
      241 gcccgtgaac tccgtaagaa tcgttttttg gcgtgccagt gcaaaccatt atccgacctc
      301 aaaattaagg tcattaaccg tgcggaggga cgtgcttcac atccccccaa acgtttctcg
      361 actcgagtag ttagtaagcg cttcctctct gacgagatgt ttgagctgcg acttgaagcg
      421 gaacagaaag tggtgttttc accagggcaa tattttatgg ttgacgtgcc tgaactcggc
      481 accagagcat actccgcggc aaaccctgtt gatggaaaca cactaacgct gatcgtaaaa
      541 gcagtgccga atgggaaggt atcctgcgca ctcgcaaatg aaactattga aacacttcag
      601 ttggatggtc cttacgggct gtcagtatta aaaactgcgg atgaaactca atccgtcttt
      661 atcgctgggg ggtcaggtat cgcgccgatg gtgtcgatgg tgaatacgct gattgcccaa
      721 gggtatgaaa aaccgattac ggtgttttac ggttcacggc tagaagctga actggaagcg
      781 gccgaaaccc tgtttgggtg gaaagaaaat ttaaaactga ttaatgtgtc gtcgagcgtg
      841 gtgggtaact cggagaaaaa gtatccgacc ggttatgtcc atgagataat tcctgaatac
      901 atggaggggc tgctaggtgc cgagttctat ctgtgcggcc cgccgcagat gattaactcc
      961 gtccagaagt tgcttatgat tgaaaataaa gtaccgttcg aagcgattca ttttgatagg
     1021 ttcttttaaa attaataagc aatagttggt tttagtagaa ttttcagtgg cgtaatgtcg
     1081 gcgctaagga actccgaata agcctgcacc aatacggcag tagcctgttc aatgtgttct
     1141 cagcgcagcc gcagccagct gattcgtcca gtagcccggg
//