From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: D.pseudoobscura Adh gene for alcohol dehydrogenase
Date: 28 Dec 92 21:27:54 GMT
Distribution: bionet
Checksum: 61099 8

LOCUS       DROADHGN69   3506 bp ds-DNA             INV       01-DEC-1992
DEFINITION  D.pseudoobscura Adh gene for alcohol dehydrogenase
ACCESSION   X64478
KEYWORDS    ADH gene; alcohol dehydrogenase.
SOURCE      Drosophila pseudoobscura DNA.
  ORGANISM  Drosophila pseudoobscura
            Eukaryota; Animalia; Metazoa; Arthropoda; Uniramia; Insecta;
            Pterygota; Neoptera; Holometabola; Diptera; Brachycera;
            Cyclorrhapha; Schizophora; Drosophiloidea; Drosophilidae.
REFERENCE   1  (bases 1 to 3506)
  AUTHORS   Schaeffer,S.W. and Miller,E.L.
  TITLE     Estimates of gene flow in Drosophila pseudoobscura determined from
            nucleotide sequence analysis of the alcohol dehydrogenase region
  JOURNAL   Genetics 132, 471-480 (1992)
  STANDARD  full automatic
COMMENT     *source: strain=PS245; *source: isolate=Kiabab National Forest,
            Arizona; *source: chromosome=IV section 88;
               From EMBL    entry DPADHGN69;  dated 16-OCT-1992.
FEATURES             Location/Qualifiers
     mRNA            join(40..119,923..1028,1092..1496,1557..1972)
                     /note="adult mRNA (Adh)"
     mRNA            join(890..1028,1092..1496,1557..1972)
                     /note="larval mRNA (Adh)"
     promoter        8..14
                     /note="adult promoter (Adh)"
     prim_transcript 40..1972
                     /note="adult primary transcript (Adh)"
     exon            40..119
                     /note="adult exon 1a"
     intron          120..922
                     /note="adult intron"
     prim_transcript 890..1972
                     /note="larval primary transcript (Adh)"
     exon            890..1028
                     /note="larval exon 1"
     exon            923..1028
                     /note="adult exon 1b"
     intron          1029..1091
                     /number=1
     exon            1092..1496
                     /number=2
     intron          1497..1556
                     /number=2
     exon            1557..1972
                     /number=3
     polyA_signal    1949..1954
     polyA_site      1972
     CDS             join(936..1028,1092..1496,1557..1823)
                     /EC_number="1.1.1.1"
                     /gene="Adh"
                     /product="alcoholdehydrogenase"
                     /codon_start=1
                     /translation="MSLTNKNVVFVAGLGGIGLDTSRELVKRNLKNLVILDRIDNPAA
                     IAELKAINPKVTITFYPYDVTVPVAETTKLLKTIFAQVKTIDVLINGAGILDDHQIER
                     TIAVNYTGLVNTTTAILDFWDKRKGGPGGIICNIGSVTGFNAIYQVPVYSGSKAAVVN
                     FTSSLAKLAPITGVTAYTVNPGITKTTLVHKFNSWLDVEPRVAEKLLEHPTQTSQQCA
                     ENFVKAIELNKNGAIWKLDLGTLEPITWTQHWDSGI"
BASE COUNT      976 a    806 c    799 g    925 t
ORIGIN      
        1 cgtagtgtat ttaaggcact gcacatgtcg aggaccacga ttattgtcta cagtgcagtc
       61 agtagttgtc aggtgcagca ggcggaccag catttcttta aatctactca atcgatcaag
      121 taagtaactg taatccaagt acagtcggcc catcccactc ggtcactcgg ttctgccgta
      181 actcaagaaa tttcttgata gcgcagttcc agttgtagaa cgtcgtttaa aaaaacatct
      241 cgttggagct tgggctgaaa aacattcgag ttgggcgtag aaaatttcaa atataaaata
      301 tcaaaaaaga gaaaatatct tcccaaagca gctgtccatt ttttgttaaa gtgatagatc
      361 aaaaattcgg atgacgtaca ttcctagtgc acttcataca aataatttcc tcaattctaa
      421 ttctacaaga agcacttttg gctcttgtcc agaaaaaaca atttgtttac acttttcggt
      481 tgataacaga ggcggaccga cggctaagta acataacagt cgcctgttaa gtaatgccct
      541 cactctctgt tagatggccg cgcgtgtcga ctccgataga ccagcagaca cgcagtagtt
      601 ttcctcaaga acctgaactc taaacataga cataatttac gataccttga gactcaaata
      661 aaaatacaag tatgagtggt attctattcc tgattagact cgcacacaca cacgtacgag
      721 tattaacata aatttgccgg caaataacca aaacaaaacg aacgcaaacg atccgaacac
      781 gtaatgcgag agataagaaa cagaaaagct cgacgtgaaa gaagcttctg gcggaagctc
      841 ttgccgaact ccctataaat acgagacaga aaaccagaat aaatataata acaaactgtt
      901 gaattgtccc atcgattccc agatcaaaag acagaatgtc tctcaccaac aagaacgtcg
      961 ttttcgtggc cggtctgggc ggcattggcc tggacaccag ccgggagttg gtcaagcgta
     1021 atctgaaggt aagagagaac gaattccatg gaatctatgg aatcctaaat ttaaaaattt
     1081 atatttttta gaacctggtc atcctggatc gcattgacaa tccggctgcc attgccgaac
     1141 tgaaggcaat caatcccaag gtgaccatca ccttctatcc ctacgatgtg actgtgcccg
     1201 tcgctgagac caccaagctc ctgaagacca tctttgccca ggtgaagaca atcgatgtcc
     1261 tgatcaacgg tgctggcatc ctggacgatc atcagatcga gcgcaccatt gccgttaact
     1321 acacgggcct ggtcaacacc accacagcca ttctggactt ctgggacaag cgcaagggcg
     1381 gcccaggcgg catcatttgc aacattggct ccgtcaccgg tttcaatgcc atctaccagg
     1441 tgcccgttta ctccggctcc aaggcggcgg tggttaactt tacctcctcc ctggcggtaa
     1501 gtaatccaac gtttttccta ctccccaaaa ctaaatattt ctttgaaatc ccatagaaac
     1561 tggctcccat tactggtgtc actgcttaca ctgtcaatcc tggcatcact aagaccactc
     1621 tggtccacaa attcaactcg tggctggatg tggagccccg tgtggcagag aagctgctcg
     1681 agcatcccac ccagacctct cagcagtgcg ccgagaactt tgtgaaggcc attgagctga
     1741 acaagaacgg tgccatctgg aagttggatc tgggcacctt ggagcccatc acatggaccc
     1801 agcactggga ctcgggcatc taaaccatcc cagagactct atgggacatg gcgttttagc
     1861 ttttagtttc tttttttcca ctcaattgtt acgtatatac atacacatat ggcaataagg
     1921 ctgatttgaa ccccgttttg aatatgataa tacaaaatta tatttgagaa atttcaacaa
     1981 aatcgataag agctatagct ctcttttaat taaaaaaaac tagataaaaa gagcaatggt
     2041 cagtggtggt cagtggtcgt tgtggcctct atcgatttcc acacaaaaac tttactgttt
     2101 agtaatagaa aagaacttga gaaaggcagc caaaagaatg tacgatctga cgggtaagca
     2161 tgtctgctat gtggctgact gcggtggcat tgcactggag accagcaagg ttctcatgac
     2221 caagaatata gcggtgagtg gtagagtggt ggtgtagaga gtggagagtg gttctggttc
     2281 ttaaacaagt ctctagtctc tagtctctag tctctagtct ctagtctcta gtctcttggc
     2341 tgctgctggt agctgtcgaa actaattaag ttaatgactt tttcatgttt tgtttttgtc
     2401 cgctgacttt tgctgttcaa agctacaatt aagtttatgt tttgagtttg atctactttt
     2461 gcttaaggga agtactttcg attacggatt gctggcatat aaaaatagat accaaggaat
     2521 gtgcctcata tcatattgat tccatcttga ttctttgccc agaaactggc catccttcag
     2581 agcgtggaaa accccccggc catcgctcag ttacaatcga ttaagcacag cacacagatc
     2641 ttcttctgga ccttcgatgt gaccatggcc cgcgaggaga tgaagaagta cttcgatgag
     2701 gtcatggtcc agatggacta catcgatgtg ctgatcaatg gggccaccct gtgcgatgag
     2761 cggaacattg atgccaccat caatacgaat ctgaccggca tgatgaacac cgtggccact
     2821 gtgctgccct atatggaccg aaagatgggc ggttcgggtg gactgatcgt gaatgtcacc
     2881 tccgtcatag gactggatcc atcgccagtc ttttgtgcgt acagtgcctc aaagtttggt
     2941 gtgattgggt tcacccgaag tctagcggtg agtgggcgag atcttaactt gaaaatgctc
     3001 tctaatgtct tccattcttt cgcaggatcc cctgtattac acccaaaatg gtgtggctgt
     3061 catggccgtc tgctgtggtc ccaccaaagt gtttgtcgat cgcgaactga ctgccttcct
     3121 ggaatacggc cagtcctttg ccgatcgcct gcgtcgtgcc ccctgccaat cgacggccgt
     3181 ctgcggccag aatatagtaa atgccatcga gagatcggag aacggacaga tctggattgc
     3241 cgacaagggc ggcctggagt cggtggccct ccactggtat tggcatatgg ccgatcagtt
     3301 tgtgaactac atgcagagca ctgacgatga ggatcaggag ttttttttgg ggcagcgatg
     3361 aggcatgcct tgatccacgg aataaaacat caaatgtgtc acaaaaagta ggcaacttgt
     3421 tttgtgtgtt tggaaataga tttgaaatat tttgaggtca tatgaataat gacataaatg
     3481 agggtgcctg aaagtatgcc aagttt
//