From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: E.coli str operon with rpsL and rpsG genes coding for ribosomal
Date: 9 Apr 92 10:02:51 GMT
Distribution: bionet
Checksum: 15736     4

LOCUS       ECOSTR1      1016 bp ds-DNA             BCT       09-APR-1992
DEFINITION  E.coli str operon with rpsL and rpsG genes coding for ribosomal
            proteins S12 and S7.
ACCESSION   J01688
KEYWORDS    ribosomal protein; ribosomal protein S12; ribosomal protein S7;
            rpsG gene; rpsL gene; str operon.
SEGMENT     1 of 4
SOURCE      Escherichia coli DNA.
  ORGANISM  Escherichia coli
            Prokaryota; Bacteria; Gram-negative facultatively anaerobic rods;
            Enterobacteriaceae.
REFERENCE   1  (bases 1 to 305)
  AUTHORS   Post,L.E., Arfsten,A.E., Reusser,F. and Nomura,A.
  TITLE     DNA sequences of promoter region for the str and spc ribosomal
            protein operons in E. coli
  JOURNAL   Cell 15, 215-229 (1978)
  STANDARD  full staff_review
REFERENCE   2  (bases 173 to 1016)
  AUTHORS   Post,L.E. and Nomura,M.
  TITLE     DNA sequences from the str operon of Escherichia coli
  JOURNAL   J. Biol. Chem. 255, 4660-4666 (1980)
  STANDARD  full staff_review
REFERENCE   3  (sites)
  AUTHORS   Timms,A.R., Steingrimsdottir,H., Lehmann,A.R. and Bridges,B.A.
  TITLE     Mutant sequences in the rpsL gene of Escherichia coli B/r:
            mechanistic implictions for spontaneous and ultraviolet light
            mutagenesis.
  JOURNAL   Mol. Gen. Genet. 232, 89-96 (1992)
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     mRNA            234..>1016
                     /note="str operon mRNA"
     CDS             303..677
                     /note="ribosomal protein S12 /nomgen='rpsL'"
                     /codon_start=1
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     CDS             774..>1016
                     /note="ribosomal protein S7 /nomgen='rpsG'"
                     /codon_start=1
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVG"
BASE COUNT      259 a    247 c    246 g    264 t
ORIGIN      HindII site.
        1 gacggtaacc gctaccttga aagtctgcgt aatgccccca ttaaggtcta tgccctgaac
       61 gaagacctta ttgcccgcgg tttgactggt caaatttcga acgacatcat tctcattgac
      121 tatactgatt tcgtcagact tacggttaag caccccagcc agatggcctg gtgatgatgg
      181 cgggatcgtt gtatatttct tgacaccttt tcggcatcgc cctaaaattc ggcgtcctca
      241 tattgtgtga ggacgtttta ttacgtgttt acgaagcaaa agctaaaacc aggagctatt
      301 taatggcaac agttaaccag ctggtacgca aaccacgtgc tcgcaaagtt gcgaaaagca
      361 acgtgcctgc gctggaagca tgcccgcaaa aacgtggcgt atgtactcgt gtatatacta
      421 ccactcctaa aaaaccgaac tccgcgctgc gtaaagtatg ccgtgttcgt ctgactaacg
      481 gtttcgaagt gacttcctac atcggtggtg aaggtcacaa cctgcaggag cactccgtga
      541 tcctgatccg tggcggtcgt gttaaagacc tcccgggtgt tcgttaccac accgtacgtg
      601 gtgcgcttga ctgctccggc gttaaagacc gtaagcaggc tcgttccaag tatggcgtga
      661 agcgtcctaa ggcttaatgg ttctccgtta agtaaggcca aacgttttaa cttaaatgtc
      721 aaactaaact cgtagagttt tggacaatcc tgaattaaca acggagtatt tccatgccac
      781 gtcgtcgcgt cattggtcag cgtaaaattc tgccggatcc gaagttcgga tcagaactgc
      841 tggctaaatt tgtaaatatc ctgatggtag atggtaaaaa atctactgct gaatctatcg
      901 tatacagcgc gctggagacc ctggctcagc gctctggtaa atctgaactg gaagcattcg
      961 aagtagctct cgaaaacgtg cgcccgactg tagaagttaa gtctcgccgc gttggt
//