From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Escherichia coli DNA for chloramphenicol acetyl transferase.
Date: 29 Jan 93 05:45:43 GMT
Distribution: bionet
Checksum: 48810 6

LOCUS       ECOCATRP12   2247 bp ds-DNA             BCT       29-JAN-1993
DEFINITION  Escherichia coli DNA for chloramphenicol acetyl transferase.
ACCESSION   D14132
KEYWORDS    chloramphenicol acetyl transferase; ribosomal protein S12.
SOURCE      Escherichia coli (strain K12) DNA.
  ORGANISM  Escherichia coli
            Prokaryotae; Bacteria; Gracilicutes; Scotobacteria; Facultatively
            anaerobic rods; Enterobacteriaceae.
REFERENCE   1  (bases 1 to 2247)
  AUTHORS   Hashimoto-Gotoh,T.
  JOURNAL   Unpublished (2048)
  STANDARD  full automatic
COMMENT     Submitted (20-Jan-1993) to DDBJ by: Tamotsu Hashimoto-Gotoh
            Institute for Neurological Diseases and Geriatrics Dept. of
            Biochemistry and Molecular Genetics Kyoto Prefectural University of
            Medicine Kawaramachi-Hirokoji Kajii-cho 465 Kamigyo-Ku Kyoto 602
            Japan Phone:  075-251-5797 Fax:    075-251-5799.
FEATURES             Location/Qualifiers
     CDS             1..375
                     /product="Ribosomal Protein, S12"
                     /label=rpsLplus
                     /codon_start=1
                     /note="Synethetic gene driven by trp promoter, miltiple
                     cloning sites, fivemissense mutations at aa positions, 32,
                     51, 92, 110, 112, a single ambermutation at aa position
                     28"
     transposon      complement(404..1342)
                     /label=Tn9
                     /partial
     mutation        1308
                     /note="'c' in mutation"
     mutation        1316
                     /note="'c' in mutation"
     mutation        1613
                     /note="'c' in mutation"
     promoter        2170..2247
                     /standard_name="trp promotor/operater"
                     /label=POtrp
     CDS             complement(491..1150)
                     /label=cat
                     /product="chloramphenicol acetyl transferase"
                     /codon_start=1
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHTEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
BASE COUNT      586 a    565 c    550 g    546 t
ORIGIN      
        1 atggcaacag ttaaccagct ggttcgaaag ccgcgagctc gtaaagtggc caaatctaac
       61 gttccggctc tcgaggcatg cccgtagaag cgtggcatat gcacacgcgt atacactact
      121 actccgaaga aaccgaattc agcgctgcgc aagctttgcc gcgtacgcct gaccaacggt
      181 ttcgaggtca cctcatatat aggtggtgaa ggacacaacc tgcaggaaca ctctgttatc
      241 ctgatcagag gcggccgcgt taaagatctg cccgggatcc ggtaccacac cgtccgcggc
      301 gctctagact gctccggagt aaaggaccgt cgacaggatc gatcgaaata cggtgtaaaa
      361 cgtccgaagg cctaatagaa gctagcttgg cactgggcca agctcgaatt tctgccattc
      421 atccgcttat tatcacttat tcaggcgtag caccaggcgt ttaagggcac caataactgc
      481 cttaaaaaaa ttacgccccg ccctgccact catcgcagta ctgttgtaat tcattaagca
      541 ttctgccgac atggaagcca tcacagacgg catgatgaac ctgaatcgcc agcggcatca
      601 gcaccttgtc gccttgcgta taatatttgc ccatagtgaa aacgggggcg aagaagttgt
      661 ccatattggc cacgtttaaa tcaaaactgg tgaaactcac ccagggattg gctgagacga
      721 aaaacatatt ctcaataaac cctttaggga aataggccag gttttcaccg taacacgcca
      781 catcttgcga atatatgtgt agaaactgcc ggaaatcgtc gtggtattca ctccagagcg
      841 atgaaaacgt ttcagtttgc tcatggaaaa cggtgtaaca agggtgaaca ctatcccata
      901 tcaccagctc accgtctttc attgccatac gaaattccgt atgagcattc atcaggcggg
      961 caagaatgtg aataaaggcc ggataaaact tgtgcttatt tttctttacg gtctttaaaa
     1021 aggccgtaat atccagctga acggtctggt tataggtaca ttgagcaact gactgaaatg
     1081 cctcaaaatg ttctttacga tgccattggg atatatcaac ggtggtatat ccagtgattt
     1141 ttttctccat tttagcttcc ttagctcctg aaaatctcga taactcaaaa aatacgcccg
     1201 gtagtgatct tatttcatta tggtgaaagt tggaacctct tacgtgccga tcaacgtctc
     1261 attttcgcca aaagttggcc cagggcttcc cggtatcaac agggacacca ggatttattt
     1321 attctgcgaa gtgatcttcc gttcgacgga gttccactga gcgtcagacc ccgtagaaaa
     1381 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     1441 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     1501 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta
     1561 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     1621 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     1681 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     1741 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagcatt gagaaagcgc
     1801 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     1861 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     1921 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     1981 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     2041 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     2101 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     2161 ggaagaagca ttctgaaatg agctgttgac aattaatcat cgaactagtt aactagtacg
     2221 caagttcacg taaaaagggt atcgacc
//