From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: D.pseudoobscura Adh gene for alcohol dehydrogenase
Date: 28 Dec 92 21:26:59 GMT
Distribution: bionet
Checksum: 35710 8

LOCUS       DROADHGN32   3459 bp ds-DNA             INV       01-DEC-1992
DEFINITION  D.pseudoobscura Adh gene for alcohol dehydrogenase
ACCESSION   X62212
KEYWORDS    ADH gene; alcohol dehydrogenase.
SOURCE      Drosophila pseudoobscura DNA.
  ORGANISM  Drosophila pseudoobscura
            Eukaryota; Animalia; Metazoa; Arthropoda; Uniramia; Insecta;
            Pterygota; Neoptera; Holometabola; Diptera; Brachycera;
            Cyclorrhapha; Schizophora; Drosophiloidea; Drosophilidae.
REFERENCE   1  (bases 1 to 3459)
  AUTHORS   Schaeffer,S.W. and Miller,E.L.
  TITLE     Estimates of gene flow in Drosophila pseudoobscura determined from
            nucleotide sequence analysis of the alcohol dehydrogenase region
  JOURNAL   Genetics 132, 471-480 (1992)
  STANDARD  full automatic
COMMENT     *source: strain=GB78; *source: isolate=Gundlach Bundschu Winery,
            Sonoma Valley, *source: California; *source: chromosome=IV section
            88;
               From EMBL    entry DPADHGN32;  dated 16-OCT-1992.
FEATURES             Location/Qualifiers
     mRNA            join(40..119,915..1020,1084..1488,1549..1964)
                     /note="adult mRNA (Adh)"
     mRNA            join(882..1020,1084..1488,1549..1964)
                     /note="larval mRNA (Adh)"
     promoter        8..14
                     /note="adult promoter (Adh)"
     prim_transcript 40..1964
                     /note="adult primary transcript (Adh)"
     exon            40..119
                     /note="adult exon 1a"
     intron          120..914
                     /note="adult intron"
     promoter        846..853
                     /note="larval promoter (Adh)"
     prim_transcript 882..1964
                     /note="larval primary transcript (Adh)"
     exon            882..1020
                     /note="larval exon 1"
     exon            915..1020
                     /note="adult exon 1b"
     intron          1021..1083
                     /number=1
     exon            1084..1488
                     /number=2
     intron          1489..1548
                     /number=2
     exon            1549..1964
                     /number=3
     polyA_signal    1941..1946
     polyA_site      1964
     CDS             join(928..1020,1084..1488,1549..1815)
                     /EC_number="1.1.1.1"
                     /gene="Adh"
                     /product="alcoholdehydrogenase"
                     /codon_start=1
                     /translation="MSLTNKNVVFVAGLGGIGLDTSRELVKRNLKNLVILDRIDNPAA
                     IAELKAINPKVTITFYPYDVTVPVAETTKLLKTIFAQVKTIDVLINGAGILDDHQIER
                     TIAVNYTGLVNTTTAILDFWDKRKGGPGGIICNIGSVTGFNAIYQVPVYSGSKAAVVN
                     FTSSLAKLAPITGVTAYTVNPGITKTTLVHKFNSWLDVEPRVAEKLLEHPTQTSQQCA
                     ENFVKAIELNKNGAIWKLDLGTLEPITWTQHWDSGI"
BASE COUNT      971 a    792 c    790 g    906 t
ORIGIN      
        1 cgtagtgtat ttaaggcact gcacatgtcg aggaccacga ttattgtcta cagtgcagtc
       61 agtagttgtc aggtgcagca ggcggaccag catttcctta aatctactca atcgatcaag
      121 taagtaactg taatccgagt acagtcggcc catcccactc ggttctgcag taactcaaga
      181 aatttcttga tagcgcagtt ccagttgtaa aacgtcgttt aaaaaaacat ctcgttggag
      241 cttgggttgg aaaacgttcg agttgggcgc agaaaatttt aaatataaaa taacgcaaaa
      301 gagaaaatat cttcccaaag cagctgtcca ttttgtgtta aagtgataga tcaaaaattc
      361 gtatgacgta tttcccaagt gctctttatg caaataattt cctcaattct aattctacaa
      421 gaagcacttt tggctcttgt ccagaaaaaa caatttgttt acacttttcg gttgataaca
      481 gaagcggacc gacggctaag taacataaca gtcgcctgtt aagtaatgcc ctcactctct
      541 gttagatggc cgcgcgtgtc gactccgata gaccagcaga cacgcagtag ttttcctcaa
      601 gaacctgaac tctaaacata gacataattt acgatacctt gagactcaaa taaaaataca
      661 agtatgagtg gtattctatt cctgattaga ctcgcacaca cacacgtacg agtattaaca
      721 taaatttgcc ggcaaataac caaaacaaaa cgaacgcaaa cgatccgaac acgtaatgcg
      781 agagataaga aacagaaaag ctcgacgtga aagaagcttc tggcggaagc tcttgccgaa
      841 ctccctataa atacgagaca gaaaaccaga ataaatataa taacaaactg ttgaattgtc
      901 ccatcgattc ccagatcaaa agacagaatg tctctcacca acaagaacgt cgttttcgtg
      961 gccggtctgg gcggcattgg cctggacacc agccgggagt tggtcaagcg taatctgaag
     1021 gtaagagaga acgaattcca tggaatctat ggaatcctaa atttaaaaat tcatttattt
     1081 tagaacctgg tcatcctgga tcgcattgac aatccggctg ccattgccga actgaaggca
     1141 atcaatccca aggtgaccat caccttctat ccctacgatg tgactgtgcc cgtcgctgag
     1201 accaccaagc tcctgaagac catctttgcc caggtgaaga caatcgatgt cctgatcaac
     1261 ggtgctggca tcctggacga tcatcagatc gagcgcacca ttgccgttaa ctacacgggc
     1321 ctggtcaaca ccaccacagc cattctggac ttctgggaca agcgcaaggg cggcccaggc
     1381 ggcatcattt gcaacattgg ctccgtcacc ggtttcaatg ccatctacca ggtgcccgtt
     1441 tactccggct ccaaggcggc ggtggttaac tttacctcct ccctggcggt aagtaatcca
     1501 acgtttttcc tactccccaa aactaaatat ttctttgaaa tcccatagaa actggctccc
     1561 attactggtg tcactgctta cactgtcaat cctggcatca ctaagaccac tctggtccac
     1621 aaattcaact cgtggctgga tgtggagccc cgtgtggcgg agaagctgct cgagcatccc
     1681 acccagacct ctcagcagtg cgccgagaac tttgtgaagg ccatcgagct gaacaaaaac
     1741 ggtgccatct ggaagttgga tttgggcacc ttggagccca tcacatggac ccagcactgg
     1801 gactcgggaa tctaaaccat cccagagact ctatgggaca tggcgtttta gcttttagtt
     1861 tctttttttc cactcaattg ttacgtatat acatacacat atggcaataa ggctgatttg
     1921 aaccccgttt tgaatatgat aatacaaaat tatatttgag aaatttcaac aaaatcgata
     1981 agagctatag ctctctttta attaaaaaaa actagataaa aagagcaatg gtcagtggtc
     2041 attgtggcct ctatcgattt ccacacaaaa actttactgt ttagtaatag aaaagaactt
     2101 gagaaaggca gccaaaagaa tgtacgatct gacgggtaag catgtctgct atgtggctga
     2161 ctgcggtggc attgcactgg agaccagcaa ggttctcatg accaagaata tagcggtgag
     2221 tggtagagag gtggtgtaga gagtggagag tgattctggt tcttaaacaa gtctctagtc
     2281 tctagtctct tggctgctgc tggtagctgt cgaaactaat taagttaatg actttttcat
     2341 gttttgtttt tgtccgctga cttttgctgt tcaaagctac aattaagttt atgttttgag
     2401 tttgatctac ttttgcttaa gggaagtact ttcgattacg gattgctggc atataaaaat
     2461 agataccaag gaatgtgcat catatcatat tgattccatc ttgattcttt gcccagaaac
     2521 tggccattct tcagagcgtg gaaaaccccc cggccatcgc tcagttacaa tcgattaagc
     2581 acagcacaca gatcttcttc tggaccttcg atgtgaccat ggcccgcgag gagatgaaga
     2641 agtacttcga tgaggtcatg gtccagatgg actacatcga tgtgctgatc aatggggcca
     2701 ccctgtgcga tgagcggaac attgatgcca ccatcaatac gaatctgacc ggcatgatga
     2761 acaccgtggc cactgtgctg ccctatatgg accgaaagat gggcggttcg ggtggactga
     2821 tcgtgaatgt cacctccgtc ataggactgg atccatcgcc agtcttttgt gcgtacagtg
     2881 cctcaaagtt tggtgtgatt gggttcaccc gaagtctagc ggtgagtggg cgggatctta
     2941 acttgaaaat gctctctaat gtcttccatt ctttcgcagg atcccctgta ttacacccaa
     3001 aatggtgtgg ctgtcatggc cgtctgctgt ggtcccacca aagtgtttgt cgatcgggaa
     3061 ctgactgcct tcctggcata cggccaaacc tttgccgatc gcctgcgtcg tgccccctgc
     3121 caatcgacgg ccgtctgcgg ccagaatata gttaatgcca tcgagagatc ggagaacgga
     3181 cagatctgga ttgccgacaa gggcggcctg gagtcggtgg ccctccactg gtattggcat
     3241 atggccgatc agtttgtgaa ctatatgcag agcactgacg atgaggatca ggagtttttt
     3301 ttgggccagc gatgaggcat gccttgatcc acggaataaa acatcaaatg tgtcacaaaa
     3361 agtaggcaac ttgtttgtgg gtctggaaat agatttgaaa tattttgagg tcatatgaat
     3421 aatgacataa atgagggtgc ctgaaagtat gccaagttt
//