HDTenEightyP/NetNews huggingface dataset:
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Lentinus torulosus ribosomal RNA (21LR0R) large subunit, partial.
Date: 19 Aug 92 10:01:46 GMT
Distribution: bionet
Checksum: 45836     2

LOCUS       LENRRLSAQ     232 bp ss-rRNA            RNA       19-AUG-1992
DEFINITION  Lentinus torulosus ribosomal RNA (21LR0R) large subunit, partial.
ACCESSION   M98650
KEYWORDS    large subunit ribosomal RNA.
SOURCE      Lentinus torulosus (library: Duke culture collection 613) cDNA to
            rRNA.
  ORGANISM  Lentinus torulosus
            Eukaryota; Fungi; Eumycota; Basidiomycotina; Hymenomycetes;
            Agaricales; Tricholomataceae.
REFERENCE   1  (bases 1 to 232)
  AUTHORS   Hibbett,D.S. and Vilgalys,R.
  TITLE     Phylogenetic relationships of the basidiomycete genus Lentinus
            inferred from molecular and morphological characters
  JOURNAL   Unpublished (1992)
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     rRNA            <1..>232
                     /note="Aligns to region between positions 70 to 306
                     inSaccharomyces cerevisiae large subunit nuclear rRNA."
                     /product="large subunit ribosomal RNA"
                     /citation=[1]
BASE COUNT       54 a     46 c     69 g     63 t
ORIGIN
        1 tcccctagta actgcgagtg aagcgggaaa agctcaaatt taaaatctgg cagtctttgg
       61 ctgtccgagt tgtattctgg agaagcgttt tccgcgctgg accgtgtaca agtctcttgg
      121 aatagagcgt cgtagagggt gagaatcccg tctttgacac ggactaccag tgctttgtga
      181 tgcgctctca aagagtcgag ttgtttggga atgcagctca aaatgggtgg tg
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: T.equiperdum mRNA for 20star, Bordeaux trypanozoan antigenic type
Date: 15 Aug 92 10:03:23 GMT
Distribution: bionet
Checksum: 13321     2

LOCUS       TRB20ST       758 bp ss-RNA             INV       15-AUG-1992
DEFINITION  T.equiperdum mRNA for 20star, Bordeaux trypanozoan antigenic type
            20star
ACCESSION   X55538
KEYWORDS    antigenic variation; gene conversion; surface antigen;
            variable surface glycoprotein.
SOURCE      Trypanosoma equiperdum RNA.
  ORGANISM  Trypanosoma equiperdum
            Eukaryota; Animalia; Protozoa; Sarcomastigophora; Mastigophora;
            Kinetoplastida; Trypanosomatina; Trypanosomatidae.
REFERENCE   1  (bases 1 to 758)
  AUTHORS   Thon,G., Baltz,T., Giroud,C. and Eisen,H.
  TITLE     Trypanosome variable surface glycoproteins: composite genes and
            order of expression
  JOURNAL   Genes Dev. 9, 1374-1383 (1990)
  STANDARD  full automatic
COMMENT     From EMBL    entry TE20ST;  dated 05-AUG-1992.
BASE COUNT      282 a    173 c    168 g    135 t
ORIGIN
        1 cactacctcc acaacgaagc agcgcaggca ccagtctggt ggggaggcaa atttttcaaa
       61 acggcgggca acaatgcaga catcggggcg gcaagctggg cagcgaatga cgaagccgac
      121 atcagcaaaa gcaacttcca aaagtgccac gaaacattaa agacaatact ctcaagcggc
      181 gtcgacgaca tggcaacaag cgcaaactta gcaaagatac taacaaaaaa tgcaccaggc
      241 atagcagaac taaacatcgc cagcggcaac atccgaaaaa acgtcccttc agcagaagca
      301 aaactaacac cggcagagct gacaaccctg agcaatgata tagcatcttt gccgaaagta
      361 acaggagctg atcagctgag cgatacggag caagatgcca aggacgttca agaaattcta
      421 gagatcaacg aaactgcctg caagttgggg aaagccctaa cactacaaac cacaggcaaa
      481 acagaaagcg aagataaaac tactaaagac aaatgcagcg acaaaaagga agatgaatgc
      541 aaagatggat gcgagctggt agagggggtt tgcaaaccag taaagcaagg agagggagaa
      601 aacaaagaaa aaaccggcac cacaaacacc acaggaagca attcttttgt tattaagaag
      661 gctcctcttt ggcttgcatt tttgcttttt taactttccc ctcaattttc tttaaggaaa
      721 ttttgctact tgaaaaactt tctgatatat tttaacac
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human chromosome 4 sequence-tagged site STS4-394
Date: 21 Aug 92 10:09:49 GMT
Distribution: bionet
Checksum: 55957     3

LOCUS       HUM4STS394    452 bp ds-DNA             PRI       21-AUG-1992
DEFINITION  Human chromosome 4 sequence-tagged site STS4-394
ACCESSION   L00836
KEYWORDS    STS; human chromosome 4; sequence tagged site.
SOURCE      Homo sapiens, clone p4-384 from a chromosome 4 plasmid library;
            vector pBluescript II KS + (Stratagene)
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 452)
  AUTHORS   Goold,R.D., diSibio,G., Dugaiczyk,A., Smith,K.A., Xu,H.,
            Magrane,G., Lang,D.B., Cox,D.R., Masters,S.B. and Myers,R.M.
  TITLE     Sequence-Tagged Sites for Human Chromosome 4
  JOURNAL   Unpublished (1992) see COMMENT for author address
  STANDARD  full automatic
COMMENT     PCR components: 25 ng of human genomic DNA, 10 pmol of each
            oligonucleotide, 200 micro-M dNTPs, 0.25 U Taq  polymerase (Cetus)
            in 10 micro-l of 50 mM KCl-20 mM  Tris-HCl, pH 8.3 (at room temp),
            2.5 mM MgCl-2.  Initial denaturation at 94degC for 1.5 min, then 30
            cycles of  94degC for 15 sec, 62degC for 23 sec, and 72degC for 30
            sec, followed by a final extension at 72degC for 3.5 min, using a
            Perkin-Elmer 9600 thermocycler. PCR-amplified product size 156 bp.
            Sequence submitted by:
            Human Genome Mapping Center
            Box 0925
            University of California San Francisco
            San Francisco, CA 94143-0925  USA
            Phone: (415) 502-1612   Fax: (415) 476-8391
            e-mail: hgmcprobes@cgl.ucsf.edu
FEATURES             Location/Qualifiers
     misc_feature    73..97
                     /label=Primer1
     misc_feature    complement(205..228)
                     /label=Primer2
BASE COUNT      147 a    106 c     69 g    127 t      3 others
ORIGIN
        1 gatctcacac acacgcgcgc gcgcgcacac acacacacac acacacactg ccttttacta
       61 gtaaaatctt tcattcttgg aagtcacaaa cactacaaat tccctggagc taggagttcc
      121 cagcaatcct gaatganaca attagggcat agccagaaag gagaaagttg ttgcctacag
      181 cctagagaat ttcccaagga cctattgaaa atgactgctg cctaaaccac agttacaaaa
      241 ctcagtacac tcacaaataa tgcctattct atgttttatc ttaaatatct gttgtttcct
      301 tgattatctc tgaagatata tatacatact tctaatttgn ggagtacttc agcaacagaa
      361 taaaaacctc atccacattg taaagtgctt aaagtaactt tcccatgtta attgntcttg
      421 gctttcattc ataagaaata gtcattgggt cc
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human chromosome 4 sequence-tagged site STS4-134
Date: 21 Aug 92 10:06:51 GMT
Distribution: bionet
Checksum: 08191     3

LOCUS       HUM4STS134    408 bp ds-DNA             PRI       21-AUG-1992
DEFINITION  Human chromosome 4 sequence-tagged site STS4-134
ACCESSION   M95258
KEYWORDS    STS; human chromosome 4; sequence tagged site.
SOURCE      Homo sapiens, clone C4-166 from Los Alamos National Laboratory
            chromosome 4 cosmid library, plate 4-1-2R, pos4B; vector SuperCos 1
            (Stratagene)
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 408)
  AUTHORS   Goold,R.D., diSibio,G., Dugaiczyk,A., Smith,K.A., Xu,H.,
            Magrane,G., Lang,D.B., Cox,D.R., Masters,S.B. and Myers,R.M.
  TITLE     Sequence-Tagged Sites for Human Chromosome 4
  JOURNAL   Unpublished (1992) see COMMENT for author address
  STANDARD  full automatic
COMMENT     PCR components: 25 ng of human genomic DNA, 10 pmol of each
            oligonucleotide, 200 micro-M dNTPs, 0.25 U Taq  polymerase (Cetus)
            in 10 micro-l of 50 mM KCl-20 mM  Tris-HCl, pH 8.3 (at room temp),
            2.5 mM MgCl-2.  Initial denaturation at 94degC for 1.5 min, then 30
            cycles of  94degC for 15 sec, 62degC for 23 sec, and 72degC for 30
            sec, followed by a final extension at 72degC for 3.5 min, using a
            Perkin-Elmer 9600 thermocycler. PCR-amplified product size 180 bp.
            Sequence submitted by:
            Human Genome Mapping Center
            Box 0925
            University of California San Francisco
            San Francisco, CA 94143-0925  USA
            Phone: (415) 502-1612   Fax: (415) 476-8391
            e-mail: hgmcprobes@cgl.ucsf.edu
FEATURES             Location/Qualifiers
     misc_feature    9..31
                     /label=Primer1
     misc_feature    complement(163..188)
                     /label=Primer2
BASE COUNT      108 a     87 c     85 g    103 t     25 others
ORIGIN
        1 gnnnncaaag cacgatcaaa gcaatggcta caaagaggtg gaagtggttt agtcaaagca
       61 aaagtggact ggtcaagagc aaggtcatgg caacagtttt tnggtatgct caaggcattt
      121 tggtngttta ctctctggag ggctaaaaaa tgataacatc tgcttattat gagagtgttt
      181 tcagaaagnt agctgaagct ttagcagaaa antccccagg aaagcctcac cagtgagtcc
      241 tnctccaccg tggcaantct cctnntcatt tctctcatca agcanggcca anttngnatg
      301 cagttntgat gcgaaattgt tgagtatccn ccctacagnc ccntgattng ggttcctact
      361 gaagtactcc ncgtcanctt nctctcaana tctctgtaaa agcctccc
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: E.coli narX gene
Date: 15 Aug 92 10:01:38 GMT
Distribution: bionet
Checksum: 64754     2

LOCUS       ECONARX       179 bp ds-DNA             BCT       15-AUG-1992
DEFINITION  E.coli narX gene
ACCESSION   X65715
KEYWORDS    narQ gene.
SOURCE      Escherichia coli DNA.
  ORGANISM  Escherichia coli
            Prokaryotae; Gram-negative facultatively anaerobic rods;
            Enterobacteriaceae.
REFERENCE   1  (bases 1 to 179)
  AUTHORS   Nohno,T., Noji,S., Taniguchi,S. and Saito,T.
  TITLE     The narX and narL genes encoding the nitrate-sensing regulators of
            Escherichia coli are homologous to a family of prokaryotic two-
            component regulatory genes
  JOURNAL   Nucleic Acids Res. 17, 2947-2957 (1989)
  STANDARD  full automatic
REFERENCE   2  (bases 1 to 179)
  AUTHORS   Cavicchioli,R.
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
REFERENCE   3  (sites)
  AUTHORS   Cavicchioli,R., Gunsalus,R.P. and Chiang,R.C.
  TITLE     Identification and Characterization of narQ...
  JOURNAL   Unpublished (1992)
  STANDARD  full staff_review
COMMENT     *source: strain=MC4100; *source: chromosome=E.coli genomic map;
            From EMBL    entry ECNARX;  dated 06-AUG-1992.
FEATURES             Location/Qualifiers
     CDS             1..179
                     /partial
                     /gene="narX"
                     /codon_start=1
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRL"
                     /partial
     old_sequence    123..156
                     /citation=[1]
BASE COUNT       31 a     45 c     52 g     51 t
ORIGIN
        1 atgcttaaac gttgtctctc tccgctcacc ctggttaatc aggttgcgct tattgtgttg
       61 ctttctactg ctattggact ggcagggatg gcggtttctg gctggctggt gcaaggcgtt
      121 cagggcagcg cccatgcgat caacaaagcg ggatcgctgc gcatgcaaag ttaccgtct
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Odocoileus virginianus texanus cytochrome b gene, 3' end.
Date: 19 Aug 92 10:02:56 GMT
Distribution: bionet
Checksum: 26665     2

LOCUS       ODOGRACB      307 bp ds-DNA             ORG       19-AUG-1992
DEFINITION  Odocoileus virginianus texanus cytochrome b gene, 3' end.
ACCESSION   M98494
KEYWORDS    cytochrome b.
SOURCE      Mitochondrion Odocoileus virginianus (chromosome Mitochondrion
            Odocoileus virginianus, sub_species texanus) adult liver DNA.
  ORGANISM  Mitochondrion Odocoileus virginianus
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Artiodactyla; Ruminantia; Pecora; Cervidae; Odocoileinae.
REFERENCE   1  (bases 1 to 307)
  AUTHORS   Carr,S.M. and Hughes,G.A.
  TITLE     The direction of hybridization between species of North American
            deer (Odocoileus) as inferred from mitochondrial cytochrome b
            sequences
  JOURNAL   J. Mammal. (1992) In press
  STANDARD  full staff_entry
FEATURES             Location/Qualifiers
     CDS             <1..298
                     /product="cytochrome b"
                     /codon_start=3
                     /translation="FGSLLGICLILQILTGLFLAMHYTSDTMTAFSSVTHICRDVNYG
                     WIIRYMHANGASMFFICLFMHVGRGLYYGSYTFLETWNIGVILLFTVMATAFVG"
BASE COUNT       88 a     75 c     47 g     97 t
ORIGIN
        1 acttcggctc tctgctagga atttgcttaa tcctacaaat tcttactggt ttattcctag
       61 caatacatta tacatccgac acaataacag cattctcctc tgtcactcac atctgccgag
      121 acgttaacta tggctgaatc attcgataca tacatgccaa tggagcatcc atattcttta
      181 tctgtctatt catacacgtg ggacgaggac tatactacgg atcatatact ttcttagaaa
      241 catgaaacat tggagtaatt ctcctattca cagttatagc cacagcattc gtagggtatg
      301 tcctacc
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Polyporus alveolaris ribosomal RNA (55LR3) large subunit, partial.
Date: 19 Aug 92 10:03:24 GMT
Distribution: bionet
Checksum: 55020     2

LOCUS       POZRRLSB      230 bp ss-rRNA            RNA       19-AUG-1992
DEFINITION  Polyporus alveolaris ribosomal RNA (55LR3) large subunit, partial.
ACCESSION   M98605
KEYWORDS    large subunit ribosomal RNA.
SOURCE      Polyporus alveolaris (library: Duke culture collection 785) cDNA to
            rRNA.
  ORGANISM  Polyporus alveolaris
            Eukaryota; Fungi; Amastigomycota; Basidiomycetes.
REFERENCE   1  (bases 1 to 230)
  AUTHORS   Hibbett,D.S. and Vilgalys,R.
  TITLE     Phylogenetic relationships of the basidiomycete genus Lentinus
            inferred from molecular and morphological characters
  JOURNAL   Unpublished (1992)
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     rRNA            <1..>230
                     /note="Aligns to region between positions 371 to 597
                     inSaccharomyces cerevisiae large subunit nuclear rRNA."
                     /product="large subunit ribosomal RNA"
                     /citation=[1]
BASE COUNT       61 a     42 c     70 g     57 t
ORIGIN
        1 gaaagatgaa aagcactttg gaaagagagt taaacagtac gtgaaattgc tgaaagggaa
       61 acgcttgaag tcagtcgcgt tgtatggaac tcagcctcgc ttttgcgtgg tgcactttct
      121 gtacgacggg ccagcatcga ttttgaccgt cggataaggt tgagggaatg tggcacttcg
      181 ggtgtgttat agccttcaat cgcatacgac ggttgggatc gaggaacgca
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Onchocerca volvulus Ras homolog of the TC4/Ran/Spi1 family, mRNA.
Date: 19 Aug 92 10:03:06 GMT
Distribution: bionet
Checksum: 54803     2

LOCUS       ONGRASA       794 bp ss-mRNA            INV       19-AUG-1992
DEFINITION  Onchocerca volvulus Ras homolog of the TC4/Ran/Spi1 family, mRNA.
ACCESSION   M98811
KEYWORDS    .
SOURCE      Onchocerca volvulus (individual_isolate Toubaru) adult cDNA to
            mRNA.
  ORGANISM  Onchocerca volvulus
            Eukaryota; Animalia; Metazoa; Arthropoda; Uniramia; Insecta;
            Pterygota; Neoptera; Holometabola; Diptera; Nematocera; Culicoidea;
            Similiidae.
REFERENCE   1  (bases 1 to 794)
  AUTHORS   Dissanayake,S., Min,X. and Piessens,W.F.
  TITLE     Filarial parasites contain a Ras homolog of the TC4/Ran/Spi1 Family
  JOURNAL   Unpublished (1992)
  STANDARD  full staff_entry
BASE COUNT      252 a    148 c    187 g    207 t
ORIGIN
        1 ggaattccat aagaagttgg gatcaatcag aaagatcact ggaaatgaat cgatcggtat
       61 gattagtata aacctcaact gggaaaacag aacgatcagt cgatcgctca agggtttgag
      121 tggtagcaat ataagtaatg gccactggtg atgatattcc gacgtttaag cttgtgcttg
      181 ttggtgatgg cggtacgggc aagacgacgt ttgttaaaag gcatcttacg ggagatcccg
      241 aaaagaaata cgttgcaaca ttgggtgtgg aagttcatcc actgatattt cacacaaaca
      301 gaggtcagat tcgattcaat gtttgggata cagctgggca agaaaaattt ggcggattgc
      361 gagatggcta ttatatacaa ggtcagtgcg ccattattat gttcgatgta acagcacgcg
      421 ttacttacaa aaatgttcca aactggcatc gtgatttagc ccgtgtatgt gaaaatatcc
      481 cgatagtttt atgtgggaat tttgtagatg tcaaggacag aaaagttaag gctaagacga
      541 tcacatttca tcgaaagaaa aatctgcagt actatgacat ctcagcaaaa agcaattata
      601 atttcgaaaa accttccctc tggctggtcc gaaaattgtt aggcgaccca aatctagaat
      661 ttgttgcaat gcctgctctt gctccacctg aagtgcagat ggatccaaca atggttgcac
      721 aatacgaaca agaaattgct gccgctgcga acgctgaact tccagacgac gacgaagatc
      781 tctaatggaa ttcc
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human Ig germline H-chain gene V region, partial cds.
Date: 22 Aug 92 10:00:39 GMT
Distribution: bionet
Checksum: 36204     2

LOCUS       HUM5IGVH      434 bp ds-DNA             PRI       22-AUG-1992
DEFINITION  Human Ig germline H-chain gene V region, partial cds.
ACCESSION   M99401
KEYWORDS    immunoglobulin heavy chain subgroup VH-III;
            immunoglobulin heavy chain variable region.
SOURCE      Homo sapiens (individual_isolate LS) blood DNA.
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 434)
  AUTHORS   Adderson,E.E., Carroll,W.L. and Shackelford,P.G.
  TITLE     The human VH3b gene subfamily is highly polymorphic
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     CDS             join(1..13,117..434)
                     /product="immunoglobulin heavy chain variable region"
                     /gene="IGHV@"
                     /codon_start=1
                     /translation="AILKGVQCEVQLVESGGGLVQPGPSLRLSCTASGFTFGYYPMSW
                     VRQAPGKGLEWVGFIRSKAYGGTTEYAASVKGRFTISRDDSKSIAYLQMNSLKTEDTA
                     VYYCTRDT"
                     /partial
     sig_peptide     1..13
                     /gene="IGHV@"
                     /codon_start=1
                     /partial
     intron          14..116
                     /gene="IGHV@"
BASE COUNT      108 a     84 c    131 g    110 t      1 others
ORIGIN
        1 gctattttaa aaggtaattc atggtgtact agagatactg agtgtgagng gacatgagtg
       61 gtagaaacag tggatatgtg tggcagtttc tgaccttggt gtttctgtgt ttgcaggtgt
      121 ccaatgtgag gtgcagctgg tggagtctgg gggaggcttg gtacagccag ggccgtccct
      181 gagactctcc tgtacagctt ctggattcac ctttgggtat tatcctatga gctgggtccg
      241 ccaggctcca gggaaggggc tggagtgggt aggtttcatt agaagcaaag cttatggtgg
      301 gacaacagaa tacgccgcgt ctgtgaaagg cagattcacc atctcaagag atgattccaa
      361 aagcatcgcc tatctgcaaa tgaacagcct gaaaaccgag gacacagccg tgtattactg
      421 tactagagac acag
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human chromosome 4 sequence-tagged site STS4-373
Date: 21 Aug 92 10:09:38 GMT
Distribution: bionet
Checksum: 25765     3

LOCUS       HUM4STS373    362 bp ds-DNA             PRI       21-AUG-1992
DEFINITION  Human chromosome 4 sequence-tagged site STS4-373
ACCESSION   L00845
KEYWORDS    STS; human chromosome 4; sequence tagged site.
SOURCE      Homo sapiens, clone p4-277 from a chromosome 4 plasmid library;
            vector pBluescript II KS + (Stratagene)
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 362)
  AUTHORS   Goold,R.D., diSibio,G., Dugaiczyk,A., Smith,K.A., Xu,H.,
            Magrane,G., Lang,D.B., Cox,D.R., Masters,S.B. and Myers,R.M.
  TITLE     Sequence-Tagged Sites for Human Chromosome 4
  JOURNAL   Unpublished (1992) see COMMENT for author address
  STANDARD  full automatic
COMMENT     PCR components: 25 ng of human genomic DNA, 10 pmol of each
            oligonucleotide, 200 micro-M dNTPs, 0.25 U Taq  polymerase (Cetus)
            in 10 micro-l of 50 mM KCl-20 mM  Tris-HCl, pH 8.3 (at room temp),
            2.5 mM MgCl-2.  Initial denaturation at 94degC for 1.5 min, then 30
            cycles of  94degC for 15 sec, 62degC for 23 sec, and 72degC for 30
            sec, followed by a final extension at 72degC for 3.5 min, using a
            Perkin-Elmer 9600 thermocycler. PCR-amplified product size 190 bp.
            Sequence submitted by:
            Human Genome Mapping Center
            Box 0925
            University of California San Francisco
            San Francisco, CA 94143-0925  USA
            Phone: (415) 502-1612   Fax: (415) 476-8391
            e-mail: hgmcprobes@cgl.ucsf.edu
FEATURES             Location/Qualifiers
     misc_feature    19..43
                     /label=Primer1
     misc_feature    complement(186..208)
                     /label=Primer2
BASE COUNT       93 a     56 c     72 g    122 t     19 others
ORIGIN
        1 tggggggcct ctctccangg gtaaccactt agattcttag taatttttat agaaaattta
       61 gcatggatat catacatctg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtgca
      121 tnccaagtct acacaaatgg ggntgtatta taaatactga tatatnccng gctctctcac
      181 tngancagaa ggctaagtga ccatctgtgn gagnccctag aaatatactg tttttttatg
      241 antgcacaat gtctttntnt ntgacagtat cacaaaatat tgtntttgag tcttatcaat
      301 aagtctgngg attattcccc tgattatntc nattataaat aactctgnaa agaacancct
      361 ag
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Homo sapiens DNA-binding protein (ZNF) gene, partial cds.
Date: 21 Aug 92 10:11:52 GMT
Distribution: bionet
Checksum: 34677     2

LOCUS       HUMZNFI       159 bp ds-DNA             PRI       21-AUG-1992
DEFINITION  Homo sapiens DNA-binding protein (ZNF) gene, partial cds.
ACCESSION   M88365
KEYWORDS    DNA binding protein; zinc finger protein.
SOURCE      Homo sapiens Placenta DNA.
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 159)
  AUTHORS   Bray,P.L., Lichter,P., Thiesen,H.J., Ward,D.C. and Dawid,I.B.
  TITLE     Characterization and mapping of human genes encoding zinc finger
            proteins
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 88, 9563-9567 (1991)
  STANDARD  full automatic
REFERENCE   2  (bases 1 to 159)
  AUTHORS   Lichter,P., Bray,P., Ried,T., Dawid,I.B. and Ward,D.C.
  TITLE     Clustering of C2-H2 zinc finger motif sequences within telomeric
            and fragile site regions of human chromosomes
  JOURNAL   Genomics 13, 999-1007 (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     CDS             1..158
                     /standard_name="zinc finger protein"
                     /product="DNA binding protein"
                     /gene="ZNF"
                     /note="putative"
                     /codon_start=1
                     /translation="IHTGEKPYTCKQCGKAVLSSWKLSRHMIMHSGDGPHKCKICGKG
                     FDFTVQHE"
                     /partial
BASE COUNT       51 a     27 c     39 g     42 t
ORIGIN
        1 attcacactg gagagaaacc ctatacatgt aaacagtgtg ggaaagcggt tttgtcatct
       61 tggaagcttt caagacacat gataatgcac agtggagatg gacctcataa atgtaagata
      121 tgtgggaaag gctttgattt cacggttcag cacgaattc
//