From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: C.pneumoniae GroE operon
Date: 29 Sep 92 10:02:51 GMT
Distribution: bionet
Checksum: 44636     6

LOCUS       CHTGROF      2694 bp ds-DNA             BCT       29-SEP-1992
DEFINITION  C.pneumoniae GroE operon
ACCESSION   X60068
KEYWORDS    groE operon.
SOURCE      Chlamydia pneumoniae DNA.
  ORGANISM  Chlamydia pneumoniae
            Prokaryotae; Gracilicutes; Scotobacteria;
            Rickettsias and Chlamydias; Chlamydiales; Chlamydiaceae.
REFERENCE   1  (bases 1 to 2694)
  AUTHORS   Puolakkainen,M.H.
  JOURNAL   Unpublished (1991)
  STANDARD  full automatic
REFERENCE   2  (bases 1 to 2694)
  AUTHORS   Kikuta,L.C., Puolakkainen,M.H., Kuo,C.-C. and Campbell,L.A.
  TITLE     Isolation and sequence analysis of the Chlamydia pneumoniae GroE
            operon
  JOURNAL   Infect. Immun. 59, 4665-4669 (1991)
  STANDARD  full automatic
COMMENT
            From EMBL    entry CPGROE;  dated 18-DEC-1991.
FEATURES             Location/Qualifiers
     -35_signal      214..220
                     /note="putative"
     -10_signal      247..252
                     /note="putative"
     RBS             463..470
                     /note="putative groES ribosomal binding site"
                     /note="putative"
     CDS             477..785
                     /note="GroES homolog; 11,315 Da"
                     /note="putative"
                     /codon_start=1
                     /translation="MSDQATTLRIKPLGDRILVKREEEEATARGGIILPDTAKKKQDR
                     AEVLVLGTGKRTDDGTLLPFEVQVGDIILMDKYAGQEITIDDEEYVILQSSEIMAVLK
                     "
     RBS             813..821
                     /note="putative groEL ribosomal binding site"
                     /note="putative"
     CDS             827..2461
                     /note="GroEL homolog; 58,284 Da"
                     /note="putative"
                     /codon_start=1
                     /translation="MAAKNIKYNEEARKKIHKGVKTLAEAVKVTLGPKGRHVVIDKSF
                     GSPQVTKDGVTVAKEIELEDKHENMGAQMVKEVASKTADKAGDGTTTATVLAEAIYSE
                     GLRNVTAGANPMDLKRGIDKAVKVVVDELKKISKPVQHHKEIAQVATISANNDSEIGN
                     LIAEAMEKVGKNGSITVEEAKGFETVLDVVEGMNFNRGYLSSYFSTNPETQECVLEDA
                     LILIYDKKISGIKDFLPVLQQVAESGRPLLIIAEEIEGEALATLVVNRLRAGFRVCAV
                     KAPGFGDRRKAMLEDIAILTGGQLVSEELGMKLENTTLAMLGKAKKVIVTKEDTTIVE
                     GLGNKPDIQARCDNIKKQIEDSTSDYDKEKLQERLAKLSGGVAVIRVGAATEIEMKEK
                     KDRVDDAQHATIAAVEEGILPGGGTALVRCIPTLEAFLPMLANEDEAIGTRIILKALT
                     APLKQIASNAGKEGAIICQQVLARSANEGYDALRDAYTDMIDAGILDPTKVTRSALES
                     ARSIAGLLLTTEALIADIPEEKSSSAPAMPSAGMDY"
     stem_loop       2493..2524
                     /note="putative terminator"
                     /note="putative"
BASE COUNT      935 a    533 c    535 g    691 t
ORIGIN
        1 aatttcacac aggaaacagc tatgaccatg attacgaatc tacggaggtg ttgtaacctc
       61 ggattcccta tctgcattag aatgggctag aatccctcac ttctactata atttctatgt
      121 ttatcaatat gcgacaggta tcatagctgc cctctccttt gctgaaaaaa ttcttacaca
      181 agaaccaggg ctctcgaact ttatttaaaa ttttttgaaa agcggaaggt ctgacttccc
      241 actcaatata ttaaagaaat cgggattgga tatgaccacc tctgcctcca ttagataaag
      301 cctttgcatt cattacgaaa aagatagacc tactctcttc cttgctttca gaagattgag
      361 tttttagcac ttaaaattat tgagtgctaa aattattgca caaaaaaaaa cgctttgtta
      421 tcgtgattgc agaaatagca aagtctctta gaacgtaaaa cataaggagc atataaatgt
      481 ctgatcaagc aacgaccctc cgaattaaac ctttgggcga tagaatcttg gtaaaaaggg
      541 aagaagaaga agccactgct cgtggaggaa tcatcttacc cgatacagca aaaaagaaac
      601 aagatcgtgc tgaggtcctt gttttaggca caggcaaacg aactgatgac ggtactctac
      661 ttcctttcga agttcaagtt ggcgatatca ttttaatgga taagtatgca ggtcaagaaa
      721 tcacaatcga tgacgaagag tatgtcattc tacagtccag tgaaatcatg gccgtcctaa
      781 aataaaatac tagtttgcag attatagaaa gttaaggaga acaacgatgg cagcgaaaaa
      841 tattaaatat aatgaagaag ccagaaaaaa aatacataaa ggggtaaaaa ctcttgcaga
      901 agcagtaaaa gttactctag gtcctaaagg acgtcacgta gttatagata agagctttgg
      961 ctctccccaa gtgactaaag atggtgttac tgtagctaaa gaaatcgagc tcgaagacaa
     1021 acatgaaaac atgggcgctc agatggtaaa agaagtcgcc agcaaaactg ctgacaaagc
     1081 aggcgacgga actacaacag caactgttct tgcagaagca atctatagcg aaggtctaag
     1141 aaatgtcact gccggtgcca atcctatgga cctaaaaaga ggtatcgaca aagccgtaaa
     1201 agttgttgtt gatgaactca aaaaaattag taaacctgta caacatcaca aagaaatcgc
     1261 tcaagtagct actatctcag caaataatga ttccgaaatc ggaaatctta ttgcagaagc
     1321 tatggaaaaa gttggtaaaa acggatccat tactgttgaa gaagctaaag gcttcgaaac
     1381 tgttctcgac gttgtagaag gaatgaactt caaccgtgga tacctctcca gctacttctc
     1441 cacaaatcca gaaactcaag aatgcgtttt agaagacgct ctgattctaa tctacgataa
     1501 aaaaatctct ggaattaaag acttccttcc agttttacaa caagtagcag aatctggacg
     1561 ccctctttta atcattgcag aagaaattga aggagaagct ttagcaactc tagtagtcaa
     1621 tagactccgt gcaggattca gagtctgtgc agtgaaagct cctggtttcg gtgacagaag
     1681 aaaagctatg ttagaagaca tcgctatcct tactggtggc caactagtta gcgaagaact
     1741 tggcatgaaa ctagagaata caactctagc aatgttagga aaagctaaga aagttatcgt
     1801 aactaaagaa gataccacaa tcgtcgaagg cttaggaaac aaacctgata tccaagctcg
     1861 atgcgacaat attaaaaaac aaatcgaaga tagcacttca gattacgaca aagaaaaact
     1921 ccaagagcgt ttagctaaac tctccggtgg tgtcgccgta atccgcgtag gagctgctac
     1981 cgaaatagag atgaaagaga aaaaagacag agtagatgat gcacaacacg caaccattgc
     2041 agctgtcgaa gaaggaatcc tccctggtgg tggaactgcc ttagttcgct gtatccctac
     2101 actagaagct ttccttccta tgctagcaaa cgaagacgaa gctattggta ctcgtattat
     2161 tctaaaagca ttaacagctc cattaaagca aattgcaagt aacgcaggta aagaaggcgc
     2221 tatcatttgt cagcaagttc tagcaagatc tgcaaatgaa ggctatgatg ctttacgtga
     2281 cgcttataca gatatgattg acgcaggaat tttagatcca actaaagtga ctcgctcagc
     2341 tctagaaagc gcacgttcta tcgcaggatt actcctcaca acagaagcct taatcgctga
     2401 tatcccagaa gagaaatctt cttcagctcc agcgatgcca agcgcaggaa tggactacta
     2461 gtctcttaag ctagtattaa caattttcca tgaggtctct tttctaacta aagaaaagag
     2521 acctttttct ttgggaatat tcatttctta atctatctta attattaaga tataaaactc
     2581 ctctgcctat atgtttaaac tgctaaaaaa tctatttctt atagggtgct gcatcgttgg
     2641 atatttctgg atgcgcaaag aaagtatcgt tgagcaatgg ctatctaacc gcct
//