From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Gallus domesticus nicotinic acetylcholine receptor alpha-5 subunit
Date: 24 Jul 92 10:05:41 GMT
Distribution: bionet
Checksum: 04714     5

LOCUS       CHKA5SUBU    1365 bp ss-mRNA            VRT       24-JUL-1992
DEFINITION  Gallus domesticus nicotinic acetylcholine receptor alpha-5 subunit
            gene, complete cds.
ACCESSION   J05642
KEYWORDS    alpha-5-subunit; nicotinic acetylcholine receptor.
SOURCE      Chicken cDNA to mRNA and DNA.
  ORGANISM  Gallus gallus
            Eukaryota; Animalia; Chordata; Vertebrata; Aves; Neornithes;
            Neognathae; Galliformes; Phasianidae.
REFERENCE   1  (bases 1 to 1365)
  AUTHORS   Couturier,S., Erkman,L., Valera,S., Rungger,D., Bertrand,S.,
            Boulter,J., Ballivet,M. and Bertrand,D.
  TITLE     Alpha 5, alpha 3, and non-alpha 3. Three clustered avian genes
            encoding neuronal nicotinic acetylcholine receptor-related subunits
  JOURNAL   J. Biol. Chem. 265, 17560-17567 (1990)
  STANDARD  full staff_entry
COMMENT     Draft entry and computer-readable sequence for [J. Biol. Chem.
            (1990) In press] kindly submitted
            by M.Ballivet, 01-AUG-1990.
FEATURES             Location/Qualifiers
     exon            <1..64
                     /gene="alpha-5"
                     /evidence=EXPERIMENTAL
     CDS             join(1..64,65..216,217..261,262..371,372..1203,1204..1365)
                     /product="nicotinic acetylcholine receptor"
                     /gene="alpha-5"
                     /evidence=EXPERIMENTAL
                     /codon_start=1
                     /translation="MPLRARSRKPGAGPAARAPQAGVSEPSFVAKSEDRLFKHLFEDY
                     QRWVRPVEHLNDTIKIKFGLAISQLVDVDEKNQLMTTNVWLKQEWIHVKLRWNPEDYA
                     GITSIRVPSDSIWIPDIVLYDNADGRFEGTSTKTVVKYDGTIAWTPPVNYKSSCTIDV
                     TFFPFDLQNCSMKFGSWTYDGSQVDIILEDYEVDKRDFFDNGEWEIVTATGSKGNRTD
                     GCCWYPFVTYSFIIRRLPLFYTLFLIIPCIGLSFLTVLVFYLPSNEAEKISLCTSVLV
                     SLTVFLLVIEEIIPSSSKVIPLIGEYLVFTMIFVTLSIVITVFAINIHHRSSSTHNAM
                     APWVRKIFLHKLPKLLCMRSHVDRYFAQKEEKGNMSGSESSRNTLEAALDSIRYITRH
                     VMKENEVREVVEDWKFIAQVLDRMFLWAFLLVSIIGSLVLFIPVIHKWASIIVPVHIG
                     STNT"
     sig_peptide     1..174
                     /gene="alpha-5"
                     /codon_start=1
     exon            65..216
                     /number=2
                     /gene="alpha-5"
                     /evidence=EXPERIMENTAL
     mat_peptide     175..1362
                     /product="nicotinic acetylcholine receptor"
                     /gene="alpha-5"
                     /codon_start=1
     exon            217..261
                     /number=3
                     /gene="alpha-5"
                     /evidence=EXPERIMENTAL
     exon            262..371
                     /number=4
                     /gene="alpha-5"
                     /evidence=EXPERIMENTAL
     exon            372..1203
                     /number=5
                     /gene="alpha-5"
                     /evidence=EXPERIMENTAL
     exon            1204..>1365
                     /gene="alpha-5"
                     /evidence=EXPERIMENTAL
BASE COUNT      377 a    263 c    283 g    442 t
ORIGIN
        1 atgcctcttc gcgcccgttc tcggaagccc ggagccggcc cggccgcccg cgcccctcag
       61 gcgggtgtat ctgaaccttc ttttgttgcc aagagtgaag atcgtttgtt taaacattta
      121 ttcgaagact atcaaagatg ggttcgcccg gtggagcact tgaatgatac aataaaaatc
      181 aagtttggtc ttgcaatctc gcagctagta gatgtggatg agaagaatca actgatgacc
      241 acgaatgtct ggctgaaaca ggagtggata catgtaaaat tacgttggaa ccctgaagat
      301 tatgctggaa taacatctat tcgtgtccca tcagattcta tttggattcc agacattgtg
      361 ttatatgaca atgcagatgg acgttttgag gggacgtcta ctaaaactgt ggtaaaatat
      421 gatggcacca ttgcttggac tccaccagta aattataaaa gttcttgtac tattgacgta
      481 accttctttc cctttgacct ccaaaactgc tctatgaaat ttggttcctg gacttacgat
      541 ggctcccagg ttgatataat tttagaagat tatgaagttg acaaaagaga cttttttgat
      601 aatggagaat gggaaatagt gactgcaaca gggagcaaag gaaatagaac tgatggatgc
      661 tgctggtatc cctttgttac atattcattt ataattagac gtttaccact tttttacacg
      721 ttgtttctca tcattccttg tattggactt tcttttctaa ctgtcctcgt cttctatctt
      781 ccttcaaatg aagctgaaaa aatttccctt tgcacttcag tcctggtatc tctgactgtt
      841 tttcttcttg tcattgaaga gattattcca tcgtcttcta aagttatccc acttatagga
      901 gaatacttgg tgtttactat gatatttgtg acattgtcca ttgtgataac tgtctttgct
      961 atcaatattc atcaccgttc ttcatctaca cacaatgcca tggcaccttg ggttcgcaag
     1021 atatttcttc acaaacttcc caagctgctg tgcatgagaa gtcatgtaga tagatacttt
     1081 gctcagaagg aggaaaaagg aaacatgagt ggatcagaat cgtctagaaa caccttggaa
     1141 gcagctctag actctatccg atatatcaca aggcatgtta tgaaggaaaa tgaagttcgt
     1201 gaggttgttg aagactggaa atttattgct caggtgcttg atcgaatgtt cttatgggcc
     1261 tttcttctgg tttcaataat tggatcactt gtgttattta ttcctgttat tcataaatgg
     1321 gcaagtataa tagtacctgt gcatataggc agtacaaata cgtaa
//