HDTenEightyP/NetNews huggingface dataset:
From: suter@VAX.MPIZ-KOELN.MPG.dbp.de
Newsgroups: bionet.molbio.methds-reagnts
Subject: rna from seeds
Date: 16 Nov 92 19:31:38 GMT
Distribution: bionet
Content-Identifier: 12
Original-To: methods@uk.ac.daresbury
Conversion: Prohibited


Subj:	rna from seeds
--------------------------------------------------------------------------------

i am repeating this question on behalf of detlef becker, who is getting
rather frustrated....

he is trying to isolate mRNA from tobacco seeds. he has already tried two
classes of methods: guanidinium and phenol. basically what happens is that
everything turns an ugly kind of brown and is very sticky.
to circumvent this, he has added vitamin C and triton: total RNA looks 
better then.
but he is unable to isolate mRNA with either dynabeads or oligo dT cellulose...
both these matrices are attacked by his total RNA mix and partly
destroyed (this could be a pH effect: the Fe++ may dissolve from the
magnetobeads at low pH).

as i understood, he has learned from others in the mpi that they have 
practically the same problems with RNA from the seeds of other plants 
(rape i believe).

could anybody suggest what substances in seeds produce these problems ?
does anybody have a sure-fire method, that (s)he or a clever colleague
has used already ??? (yeah, yeah, he will accept a kit
also, as long as it works).

please post your answers to me or the newsgroup, i will forward them to detlef.



 
cheers,

clemens





===============================================================================
Clemens Suter-Crazzolara, PhD
Max-Planck-Institut fuer Zuechtungsforschung
Abteilung Genetische Grundlagen der Zuechtungsforschung
Carl-von-Linne Weg 10
5000 Koeln 30
Tel. xx49-221-5062.221           fax. xx49-221-5062.21
suter@vax.mpiz-koeln.mpg.dbp.de
===============================================================================
From: cochrane@icbr.ufl.edu
Newsgroups: bionet.molbio.evolution
Subject: Two positions
Date: 17 Nov 92 15:47:41 GMT
Organization: ICBR


  Below is a description of two tenure-track positions currently
open in our department.  Inquiries and applications are welcome.

Evolutionary Geneticist and Environmental Microbiologist.  The
University of South Florida and the Department of Biology has
two full-time, 9 month tenure-track positions at the level of
Assistant or Associate Professor.  Candidates need a Ph.D. and
postdoctoral research experience; are expected to train graduate
students and  establish research programs that are
molecularly-oriented and externally-funded.  Teaching will be
undergraduate genetics or microbiology and in specialty graduate
courses.  Intra- and interdepartmental collaboration is
facilitated through the Institute for Biomolecular Science and
the Center for Urban Ecology.  Title and salary will be
commensurate with qualifications. USF is a comprehensive
research and teaching university.  The Department is
multi-disciplinary with 35 faculty members.  Applicants should
forward a letter of intent, curriculum vitae, reprints,
statements of research and teaching interests, and should
arrange for 3 letters of recommendation to be forwarded. 
Deadline for receipt of all materials is 12/18/92.  Address 
materials to Chair of the appropriate Search Committee: 
Department of Biology, University of South Florida, Tampa,
Florida 33620-5150.

Positions are contingent upon funding.  USF is an EO/AA employer
and encourages applications from minorities and women.  If 
accommodation because of a disability is required to participate
in the application/selection process, notify the Chair in
advance.


For further information, contact Bruce Cochrane at
coch@ultrix.csc.usf.edu
From: blum@aeolus.ethz.ch (Blum)
Newsgroups: bionet.molbio.embldatabank
Subject: datasearch
Date: 20 Nov 92 14:22:06 GMT
Distribution: bionet
Content-Identifier: 11
Original-To: embl-db@uk.ac.daresbury (IPM Return Requested)
Conversion: Prohibited

Does anyone know  how to obtain the "BLAST" programmme to perform searche
much quicker than provided by "FASTA"? 
Newsgroups: bionet.molbio.embldatabank
From: raja@mayo.edu (Raja Muthupillai, BIR Grad Student, Dr. Robb)
Subject: Request for information on resources  
Reply-To: raja@mayo.edu
Organization: Mayo Foundation
Date: Sun, 15 Nov 92 23:36:24 GMT


Hi,

I am new to the newsgroups in the field of molecular biology.
I am very much interested in the resources availble in the network,
for researchers in molecular biology.  I'd greatly appreciate any
relevant information, like  major ftp sites, softwares available, etc

If anyone wants to send it directly, please do so.
My email address  is raja@mayo.edu

Thanks,
Raja.

Newsgroups: bionet.molbio.embldatabank
From: danj@merlot.gwu.edu (Dan Jacobson )
Subject: Re: datasearch
Organization: George Washington University, Washington, D.C.
Distribution: bionet
Date: Fri, 20 Nov 1992 18:55:21 GMT

In article <1992Nov20.143941.16564@gserv1.dl.ac.uk> blum@aeolus.ethz.ch (Blum) writes:
>Does anyone know  how to obtain the "BLAST" programmme to perform searche
>much quicker than provided by "FASTA"? 


You can get the blast code or binaries by anonymous ftp from

ncbi.nlm.nih.gov  in the pub/blast directory.

Dan Jacobson 

danj@welchgate.welch.jhu.edu
danj@chablis.gwu.edu
From: philstas@rc1.vub.ac.be (Stas Philippe)
Newsgroups: bionet.molbio.hiv
Subject: Re: AIDS Infections rates requested.
Date: 17 Nov 92 08:36:46 GMT
Organization: Vrije Universiteit Brussel, Brussels

In article <1992Nov3.012914.10984@u.washington.edu>, stick@carson.u.washington.edu (suzanne cherniack) writes:
> magnotta@icaen.uiowa.edu (Vincent Alfonso Magnotta) writes:
> 
> A simple answer...  the Centers for Disease Control in Atlanta, GA.  I'm not
> sure of the exact address, but I'm sure it's easy enough to find.
> 
> -Suzanne   
On the newsgroup sci.med.aids, a summary of the CDC AIDS articles is reviewed
each day.
Try a reply. Billy Goldberg helped me out with some scientific information requests, like popular and non-polpular articles.


Philippe

"If Man is five, Then the devil is six"
"And if the devil is six, Then GOD is seven"
"This monkey is going to heaven"
-------------------------------------------------------------
-  Philippe Stas                        philstas@vub.ac.be  -
-  Dept. of Cellular Immunology                             -
-  Free University of Brussels          tel: 32-2-359.03.58 -
-  Paardenstraat 65                     Fax: 32-2-359.03.90 -
-  B1640 St.Genesius Rode             Modem: 32-2-359.03.59 -
-  Belgium, Europe                           (2400,1,N,8,1) -
-------------------------------------------------------------
Newsgroups: bionet.molbio.genbank
From: raja@mayo.edu (Raja Muthupillai, BIR Grad Student, Dr. Robb)
Subject: Request for info on resources available
Reply-To: raja@mayo.edu
Organization: Mayo Foundation
Date: Sun, 15 Nov 92 23:38:23 GMT


Hi,

I am new to the newsgroups in the field of molecular biology.
I am very much interested in the resources availble in the network,
for researchers in molecular biology.  I'd greatly appreciate any
relevant information, like  major ftp sites, softwares available, etc

If anyone wants to send it directly, please do so.
My email address  is raja@mayo.edu

Thanks,
Raja.
From: kristoff@NET.BIO.NET (Dave Kristofferson)
Newsgroups: bionet.molbio.genbank
Subject: Re: FTP-ANONYMOUS REJECTED
Date: 18 Nov 92 22:25:25 GMT
Distribution: bionet

> In <1992Nov17.122929.1@bb1t.monsanto.com> jmrots@bb1t.monsanto.com writes:
> 
> >Hi,
> >I tried to FTP to genbank.bio.net [134.172.1.160] with ANONYMOUS and I got
> >rejected.Could someone tell me if the system has changed recently?
> 
> Try net.bio.net instead of genbank.bio.net that might help.
> 
> -=ROB=-
> 
> --
>  ~ Rob Harper                    ~ E-mail:          harper@convex.csc.fi    
>  ~ Finnish State Computer Centre ~ Molbio/software: harper@nic.funet.fi
>  ~ P.O. Box 40                   ~ Telephone:       +358 0 457 2076
>  ~ SF-02101 Espoo Finland        ~ Fax:             +358 0 457 2302
> 
> 

If  you are looking for GenBank data, genbank.bio.net is no longer the
place to go.  Please ftp to ncbi.nlm.nih.gov.

				Sincerely,

				Dave Kristofferson
				BIOSCI/bionet Manager

				kristoff@net.bio.net
From: harper@convex.csc.FI (Rob Harper)
Newsgroups: bionet.molbio.genbank
Subject: Re: FTP-ANONYMOUS REJECTED
Date: 17 Nov 92 19:15:21 GMT
Organization: Finnish Academic and Research Network Project - FUNET

In <1992Nov17.122929.1@bb1t.monsanto.com> jmrots@bb1t.monsanto.com writes:

>Hi,
>I tried to FTP to genbank.bio.net [134.172.1.160] with ANONYMOUS and I got
>rejected.Could someone tell me if the system has changed recently?

Try net.bio.net instead of genbank.bio.net that might help.

-=ROB=-

--
 ~ Rob Harper                    ~ E-mail:          harper@convex.csc.fi    
 ~ Finnish State Computer Centre ~ Molbio/software: harper@nic.funet.fi
 ~ P.O. Box 40                   ~ Telephone:       +358 0 457 2076
 ~ SF-02101 Espoo Finland        ~ Fax:             +358 0 457 2302
Newsgroups: bionet.molbio.genbank
From: dli@magnus.acs.ohio-state.edu (Dawei Li)
Subject: Consensus Sequences?
Organization: The Ohio State University
Date: Mon, 23 Nov 1992 01:41:29 GMT

     I am analysing the DNA sequence of a gene's promoter region.

     Can any one point to me a data base or other comprehensive source for 
transacting factor binding consensus sequences as well as other consensus 
sequences,for example: enhancer sequences ? Is this a FAQ?
     
     Your help will be greatly appreciated.

     Dawei
From: jmrots@bb1t.monsanto.com
Newsgroups: bionet.molbio.genbank
Subject: FTP-ANONYMOUS REJECTED
Date: 17 Nov 92 18:29:29 GMT
Organization: Monsanto Company, St. Louis, MO

Hi,
I tried to FTP to genbank.bio.net [134.172.1.160] with ANONYMOUS and I got
rejected.Could someone tell me if the system has changed recently?

Thanks
Jean

JMROTS@BB1T.MONSANTO.COM
Newsgroups: bionet.molbio.genbank
From: aarment@desire.wright.edu
Newsgroups: bionet.molbio.genbank
Subject: Old Genbank Searching Software, PUB?
Date: 23 Nov 92 08:13:22 EST
Organization:  Wright State University 

	Is the old search software for the Genbank, GenPept, and SwissProt
databases available in public domain?  I have FASTA still, but would like to be
able to find sequences in the database files by searching as per the old login:
genbank method.

aarment@desire.wright.edu
From: maize@bragg.bio.purdue.edu
Newsgroups: bionet.molbio.genbank
Subject: Re: FTP-ANONYMOUS REJECTED
Date: 18 Nov 92 17:49:31 GMT
Reply-To: maize@bragg.bio.purdue.edu
Organization: Purdue University, Dept. of Biological Sciences

In article <1992Nov17.122929.1@bb1t.monsanto.com>, jmrots@bb1t.monsanto.com writes:
>Hi,
>I tried to FTP to genbank.bio.net [134.172.1.160] with ANONYMOUS and I got
>rejected.Could someone tell me if the system has changed recently?
>
>Thanks
>Jean
>
>JMROTS@BB1T.MONSANTO.COM


Try anonymous.  (Lowercase.)  I'm not joking -- unix differentiates between
lower and uppercase letters.

Phillip
From: DSCT@db1.cc.rochester.edu (DAVID SCOTT)
Newsgroups: bionet.molbio.proteins
Subject: Re: TCA precipitations
Date: 18 Nov 92 21:53:22 GMT
Organization: University of Rochester, Rochester NY
X-News-Reader: VMS NEWS 1.11

In <199211180207.AA03982@ux1.cso.uiuc.edu> Dan_Szymanski@QMS1.LIFE.UIUC.EDU writes:

> 
>                        Subject:                               Time:7:59 PM
>   OFFICE MEMO          TCA precipitations                     Date:11/17/92
> wondering.... does TCA precipitate things in a way similar to ammonium sulfate?
>  who knows the real story?  further, what would are the characteristics of a
> protein that is soluable in low percentage solutions of TCA?  highly charged?
> h-bonding characteristics? stability/solvent acccesiblity? so many questions. 
> thanks for any responses

	TCA and ammonium sulfate do not precipitate in the same way.  TCA
irreversibly denatures proteins whereas amm. sulf. does not. Amm. sulf.
ppt.ated proteins can be readily redissolved in physiological buffers.  Can't
help with the rest. Sorry
Newsgroups: bionet.molbio.proteins
From: raja@mayo.edu (Raja Muthupillai, BIR Grad Student, Dr. Robb)
Subject: Request for info reg. resources In article 
Reply-To: raja@mayo.edu
Organization: Mayo Foundation
Date: Sun, 15 Nov 92 23:41:17 GMT


Hi,

I am new to the newsgroups in the field of molecular biology.
I am very much interested in the resources availble in the network,
for researchers in molecular biology.  I'd greatly appreciate any
relevant information.

If anyone wants to send it directly, please do so.
My email address raja@mayo.edu

Thanks,
Raja.

Newsgroups: bionet.molbio.proteins
From: karsten@asl.uni-bielefeld.de (Karsten Quast)
Subject: Looking for a protein secondary-structure databank 
Date: Fri, 20 Nov 1992 11:00:37 GMT
Organization: Universitaet Bielefeld, ASL.

Hi,

I,m looking for a databank which contains protein secondary-structure data.
I'd like to implement a neural network which predicts secondary-structure
and need very much data for the training.

Thanks in advance for any responses

					Karsten
From: srckuxw@lincoln.cri.nz
Newsgroups: bionet.molbio.proteins
Subject: peptide analysis
Date: Thu, 19 Nov 92 00:09:37 GMT  
Organization: Canty Ag & Science Centre, New Zealand.

Dear colleagues

I am interested in the glycosylation of a protein. My idea is to separate a
tryptic (or other) digest of my protein on a polyacrylamide gel, blot them on a
PVDF Membrane and screen the membrane afterwards for glycosylated peptides. Has
anybody experiance with that kind of work. I am manly interested in hints how
to bind small peptides (smaller than 10K) on membranes.
Thanks in advance
                         Urs Waefler
                         Crop & Food Research
                         Lincoln
                         New Zealand
From: ORTIGAO@rzmain.rz.uni-ulm.de (ORTIGAO FLAVIO)
Newsgroups: bionet.molbio.proteins
Subject: Solid-phase peptide modification
Date: 17 Nov 92 19:08:45 GMT
Organization: University of Ulm, Germany
X-News-Reader: VMS NEWS 1.24

Dear collegues:
I am looking for methods, compatible wiht Fmoc-chemistry to derivatize a peptide
with a handle, which could be used to attach the growing peptide covalently into
a different surface. Ideally the handle should be introduced while the peptide
is still protected. Even better it should be cleaved from support while still
protected, and would be deprotected after the coupling to the new surface.
I would appreciate any help.
Thanks in advance, Flavio
+++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++
Dr. J.F. Ramalho Ortigao
University of Ulm
Sektion Polymere
D-7900 Ulm, Germany
From: Dan_Szymanski@QMS1.LIFE.UIUC.EDU ("Dan Szymanski")
Newsgroups: bionet.molbio.proteins
Subject: TCA precipitations
Date: 18 Nov 92 02:05:04 GMT
Distribution: bionet


                       Subject:                               Time:7:59 PM
  OFFICE MEMO          TCA precipitations                     Date:11/17/92
wondering.... does TCA precipitate things in a way similar to ammonium sulfate?
 who knows the real story?  further, what would are the characteristics of a
protein that is soluable in low percentage solutions of TCA?  highly charged?
h-bonding characteristics? stability/solvent acccesiblity? so many questions. 
thanks for any responses
From: Dan_Szymanski@QMS1.LIFE.UIUC.EDU ("Dan Szymanski")
Newsgroups: bionet.molbio.proteins
Subject: TCA precipitations
Date: 20 Nov 92 15:09:37 GMT
Distribution: bionet


                       Subject:                               Time:7:59 PM
  OFFICE MEMO          TCA precipitations                     Date:11/17/92
wondering.... does TCA precipitate things in a way similar to ammonium sulfate?
 who knows the real story?  further, what would are the characteristics of a
protein that is soluable in low percentage solutions of TCA?  highly charged?
h-bonding characteristics? stability/solvent acccesiblity? so many questions. 
thanks for any responses
From: Dan_Szymanski@QMS1.LIFE.UIUC.EDU ("Dan Szymanski")
Newsgroups: bionet.molbio.proteins
Subject: competitive inhibition
Date: 18 Nov 92 02:22:03 GMT
Distribution: bionet


                       Subject:                               Time:7:59 PM
  OFFICE MEMO          competitive inhibition                 Date:11/17/92
to the net:
i am looking for a reference that will help in designing an gel mobility shift
experiment that will allow me to calculate the equilibrium binding constant of
a ligand (dna binding protein) for a competitor (different DNA polymers)
knowing the the eq. binding constant for a high affinity site with constrained
sequence.  i know im looking at around 10 -8 to 10 -9 for the high affinity
site, and the competitors will be within an order of magnitude, or two at the
most. 
sincerely,
molecular biologist with a cause,
dan
From: ebstokes@maxwell.crd.ge.com (Ed Stokes)
Newsgroups: bionet.molbio.proteins
Subject: tryp in IgG ?
Date: 16 Nov 92 16:25:58 GMT
Organization: GE Corp. Research & Development, Schenectady, NY

I am a physicist investigating the binding and optical properties
of antibodies for possible sensor applications. My understanding
is that if an IgG antibody contains tryptophan in the binding
pocket, then the perturbation of the tryptophan luminescence
properties can be used to identify an antigen binding event.

Are the sequences of IgG's from various species and for various
antigens known ? How can I know whether there are luminescent
amino acid residues in the binding pocket of a particular
antibody ? Can these properties be engineered ?

Thanks
Ed Stokes
ebstokes@crd.ge.com
From: tolvanen@klaava.Helsinki.FI (Martti Tolvanen)
Newsgroups: bionet.molbio.proteins
Subject: New PDB entries
Date: 23 Nov 92 13:53:45 GMT
Organization: University of Helsinki

I just read in Nature, Nov.  19, (360:232-239) about a crystal structure
of CD2 that has been deposited in the Protein Data Bank, and of course I
went ahead and checked the prerelease area in their ftp site
(pdb.pdb.bnl.gov).  Searching for cd2 in the NOTICE.UPDATE file or in
the filenames gave nothing, though. 

Does anyone have an idea how long it takes to process a submitted entry
into a prerelease entry?
-- 
Martti Tolvanen, Dept. Biochem., Univ. Helsinki, Finland
tolvanen@cc.helsinki.fi
From: Davison@UH.EDU (Dan Davison)
Newsgroups: bionet.molbio.proteins
Subject: Re: Looking for a protein secondary-structure databank
Date: 20 Nov 92 19:18:38 GMT
Distribution: bionet

POSTMASTER@NBRF.Georgetown.Edu said:
> Release 9.1 of NRL_3D is available through the PIR Network Request Server,
> through the PIR On-Line Access System and by FTP from the University
> of Houston server at ftp.bchs.uh.edu in the files
>   /pub/gene-server/incoming/pir33/nrl_3d-9.1-vms
>   /pub/gene-server/incoming/pir33/nrl_3d-9.1-ascii
> Our thanks to Bill Pearson and Dan Davison for their efforts in
> providing FTP access to the PIR databases.


The files are moved; they are in 
pub/gene-server/pir/pir_rel34/vms or
pub/gene-server/pir/pir_rel34/ascii
as compressed files (nrl_*.Z). Be sure to use "binary" mode when
transferring the files.

dan



-- 
dr. dan davison/dept. of biochemical and biophysical sciences/univ. of
Houston/4800 Calhoun/Houston,TX 77204-5934/davison@uh.edu/DAVISON@UHOU

-----RIP Isaac Asimov 1920-1992     I'll miss him --------------------

Disclaimer: As always, I speak only for myself, and, usually, only to
myself.
From: Carlos.Faerman@BRI.NRC.CA (Carlos Faerman)
Newsgroups: bionet.molbio.proteins
Subject: General Question
Date: 17 Nov 92 19:28:44 GMT
Distribution: bionet

I would like to find out whether a tabulation of the second-order rate
constants for enzymes is available. In other words, I would be very happy
to find a Table that lists Kcat/Km for a large set of the known
enzymes.
I look forward to hearing from you.

e-mail: carlos@bri.nrc.ca

Newsgroups: bionet.molbio.gene-linkage
From: toby@carson.u.washington.edu (Toby Bradshaw)
Subject: Re: Error analysis
Organization: University of Washington, Seattle
Date: Thu, 19 Nov 1992 16:28:17 GMT

In article <1ef0muINNlc3@gazette.bcm.tmc.edu> bwc@bcm.tmc.edu writes:
>
>If anyone has suggestions about software which detects possible errors in human linkage data, I would appreciate hearing about it.  I am not
>refering to directly detectable errors such as incompatible inheritance,
>but rather possible errors such as double recombination over a very
>short interval, or many recombinants within a family.

The new MAPMAKER 3.0 has such error detection.  The algorithms are
described in a paper (which I haven't read) by Steve Lincoln and
Eric Lander, possibly in Genomics.  Error detection becomes very
important in high-density maps, such as the mouse map, since
most errors overestimate the number of recombinants, inflate the
map distance, and cause problems ordering the markers.  MAPMAKER
3.0 should be available soon, and now will run on 32-bit PCs with
math coprocessors.

Toby Bradshaw
Department of Biochemistry and College of Forest Resources
University of Washington, Seattle
toby@u.washington.edu
From: ifenton@crc.ac.uk (I. Fenton)
Newsgroups: bionet.molbio.gene-linkage
Subject: Re: Error analysis
Date: 19 Nov 92 19:43:42 GMT
Organization: MRC Human Genome Resource Centre

In article <1992Nov19.162817.14480@u.washington.edu> toby@carson.u.washington.edu (Toby Bradshaw) writes:
[...]
>map distance, and cause problems ordering the markers.  MAPMAKER
>3.0 should be available soon, and now will run on 32-bit PCs with
>math coprocessors.
>
>Toby Bradshaw
>Department of Biochemistry and College of Forest Resources
>University of Washington, Seattle
>toby@u.washington.edu

toby,

what is soon ?  also what does 32-bit pcs mean ?  is that any intel386
or better machine ?  i would be dead interested in running this package
on a pc, even if it requires a high-end pc.  do you know what are the
memory etc requirements for this.

thanks in advance.

=============================================================================
IAIN FENTON                             | All errors of spelling, grammar &
Institute of Medical Genetics           | fact are due to a missing <Del> key
University of Wales College of Medicine |====================================
Heath Park                              | "No man should marry until he has
Cardiff  CF4 4XN                        |  studied anatomy and dissected at
U.K.                                    |  least one woman" - de Balzac
=============================================================================
Voice = +44 - 222 - 744049   | In 1912, Jack Johnson, the first black world
Fax   = +44 - 222 - 747603   | heavyweight boxing champion, was refused
Email = fenton@uk.ac.cardiff | permission to board the Titanic because of
   (or) wmgif@uk.ac.cardiff  | his colour.
   (or) ifenton@uk.ac.crc    |
=============================================================================
Newsgroups: bionet.molbio.gene-linkage
From: toby@carson.u.washington.edu (Toby Bradshaw)
Subject: Re: Error analysis
Organization: University of Washington, Seattle
Date: Thu, 19 Nov 1992 22:07:28 GMT

In article <1992Nov19.194342.1780@crc.ac.uk> ifenton@crc.ac.uk (I. Fenton) writes:
>In article <1992Nov19.162817.14480@u.washington.edu> toby@carson.u.washington.edu (Toby Bradshaw) writes:
>[...]
>>map distance, and cause problems ordering the markers.  MAPMAKER
>>3.0 should be available soon, and now will run on 32-bit PCs with
>>math coprocessors.
>>
>toby,
>
>what is soon ?

How high is up :)  Steve Lincoln was passing out request forms
for MAPMAKER 3.0 at the recent Plant Genome meeting.  Supposedly,
it will also be available from the Whitehead by anonymous ftp
(genome.wi.edu if I remember right).

>also what does 32-bit pcs mean ?  is that any intel386
>or better machine ?

Real 386 + coprocessor (as opposed to 386SX) and 486.

>i would be dead interested in running this package
>on a pc, even if it requires a high-end pc.  do you know what are the
>memory etc requirements for this.

According to Steve, MAPMAKER on a 486/4Mb is about half the speed
of a SPARCII, which is not bad considering the price differential.

Toby Bradshaw
Department of Biochemistry and College of Forest Resources
University of Washington, Seattle
toby@u.washington.edu