From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human Ig germline H-chain pseudogene V-J6-region, hybridoma AE6-5,
Date: 12 Sep 92 10:02:38 GMT
Distribution: bionet
Checksum: 14647     4

LOCUS       HUMIGHVW      778 bp ds-DNA             PRI       12-SEP-1992
DEFINITION  Human Ig germline H-chain pseudogene V-J6-region, hybridoma AE6-5,
            5' end, clone II4a.
ACCESSION   M29466
KEYWORDS    germline; immunoglobulin heavy chain; joining region;
            variable region; variable region subgroup VH-I.
SOURCE      Human (patient POP) hybridoma AE6-5 DNA, clone II4a, derived from
            B-CLL cell line UC729-6.
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 256)
  AUTHORS   Spatz,L.A., Wong,K.K., Williams,M., Desai,R., Golier,J.,
            Berman,J.E., Alt,F.W. and Latov,N.
  JOURNAL   Unpublished (1990)
  STANDARD  full staff_entry
REFERENCE   2  (bases 257 to 778)
  AUTHORS   Spatz,L.A., Wong,K.K., Williams,M., Desai,R., Golier,J.,
            Berman,J.E., Alt,F.W. and Latov,N.
  TITLE     Cloning and sequence analysis of the variable heavy (VH) and light
            (VL) chain regions of an anti-myelin/DNA antibody from a patient
            with peripheral neuropathy and chronic lymphocytic leukemia
  JOURNAL   J. Immunol. 144, 2821-2828 (1990)
  STANDARD  full staff_entry
COMMENT     Draft entry and printed sequence for [2] kindly submitted by
            L.A.Spatz, 26-OCT-1989, for release after publication.
            Columbia University, Department of Neurology BB-322, 630 W. 168th
            street, New York, NY 10032.
FEATURES             Location/Qualifiers
     CDS             join(257..302,384..>778)
                     /pseudo
                     /note="V-J6-region precursor"
                     /product="IgH"
                     /gene="IGH@"
                     /codon_start=1
                     /partial
     sig_peptide     join(257..302,384..394)
                     /gene="IGH@"
                     /codon_start=1
     misc_signal     157..164
                     /note="octamer recognition sequence"
     exon            384..>778
                     /number=2
                     /gene="IGH@"
     mat_peptide     395..>778
                     /pseudo
                     /gene="IGH@"
                     /codon_start=1
     misc_recomb     733..734
                     /note="V-region DNA end/J6-region DNA start"
BASE COUNT      193 a    197 c    216 g    172 t
ORIGIN
        1 aacacagagt tcagggatgg gtaggggatg cgtggcctct aacaggatta cggcttgaac
       61 cctcagcttc tacaattgtg tcgtccatgt gtcatgtatt tgctctttct catcctgggt
      121 caggaattgg gctattaaat agcatccttc atgaatatgc aaataactga ggtgaatata
      181 gatatctgtg tgccctgaga gcatcaccca aaaaccacac ccctccttgg gagaatccct
      241 agatcacagc tctaccatgg actggacctg gagcatcctt ttcttggtgg cggcagcaac
      301 actaacggac tccccagtcc cggggctgag agagaaacca ggccagtcat gtgagacttc
      361 acccatcctg tgtctctcca caggtgccca ctcccaggtt cgactggtgc agtctggagc
      421 tgacgtgaag aagcctgggg cctcagtgaa ggtctcctgc aaggcttctg gttacacctt
      481 taccagctat gatatcagct gggtgcgaca ggcccctgga caagggcttg agtggatggg
      541 atggatcagc gcttttaatg gtaacacaga ctatgcacag aagttccagg gcagggtcac
      601 catgaccaca gacacatcca cgagcacagc ctacacggag ctgaggagcc tgagatctga
      661 cgacacggcc gtgtattact gtgcgagaga cgggggcttg ggagtggtgg tgatagttga
      721 aacctaaagc aagtactaca acgatatgga cgtctggggc caagggacca cggtcacg
//