From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: E.coli AsnS gene for asparaginyl-tRNA synthetase
Date: 21 Dec 92 20:41:05 GMT
Distribution: bionet
Checksum: 08950 5

LOCUS       ECOASNS      2021 bp ds-DNA             BCT       18-NOV-1992
DEFINITION  E.coli AsnS gene for asparaginyl-tRNA synthetase
ACCESSION   X68192
KEYWORDS    asparaginyl-transfer RNA synthetase.
SOURCE      Escherichia coli DNA.
  ORGANISM  Escherichia coli
            Prokaryotae; Gram-negative facultatively anaerobic rods;
            Enterobacteriaceae.
REFERENCE   1  (bases 1 to 2021)
  AUTHORS   Aoki,H., Yaworsky,P.J., Patel,S.D., Margolin-Brzezinski,D.,
            Park,K.S. and Ganoza,C.
  TITLE     The asparaginyl-tRNA synthetase gene encodes one of the
            complementing factors for thermosensitive translation in the
            Escherichia coli mutant strain N4316
  JOURNAL   Eur. J. Biochem. 209, 511-521 (1992)
  STANDARD  full automatic
COMMENT     *source: strain=K12; *source: clone_library=KOHARA GENE BANK;
            *source: clone=11F5; *source: map_position=20.9;
               From EMBL    entry ECASNS;  dated 30-OCT-1992.
FEATURES             Location/Qualifiers
     -35_signal      47..52
     TATA_signal     72..77
     terminator      1504..1532
                     /note="terminator I"
     terminator      1637..1674
                     /note="terminator II"
     CDS             91..1491
                     /gene="AsnS"
                     /product="Asparaginyl-tRNA synthetase"
                     /codon_start=1
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
BASE COUNT      503 a    467 c    486 g    565 t
ORIGIN      
        1 tctgattgca gatgccaggt aacataggta tccccccatt tacgggtgga caagtgttta
       61 ttttttccga ctattaacag agagaatatt atgagcgttg tgcctgtagc cgacgtactc
      121 cagggccgtg tagccgttga cagcgaagtc accgtgcgcg gatgggtacg tacccgccga
      181 gattcaaaag ctggcatctc cttcctcgcc gtttatgacg gttcctgctt tgatcctgta
      241 caggctgtca tcaataattc tctgcccaat tacaatgaag acgtcctgcg tctgaccacc
      301 ggctgctcgg tcattgtgac gggtaaagtc gtggcgtcgc cgggccaggg gcaacaattt
      361 gaaattcagg ccagcaaggt tgaagttgct ggttgggttg aagatccaga cacttacccg
      421 atggcggcaa aacgccacag cattgagtat ctgcgtgaag tcgctcacct gcgtccgcgc
      481 acaaacctga ttggtgccgt cgcgcgcgtt cgccatacgc tggcgcaggc gctgcatcgc
      541 ttctttaacg agcagggatt cttctgggtt tcaacgccac tgattaccgc atctgatacc
      601 gaaggtgcag gcgaaatgtt ccgcgtttct acgctggatc tggaaaacct gccgcgtaac
      661 gatcagggca aagtggattt cgacaaagac ttctttggta aagagtcttt cctgaccgta
      721 tctggccagt tgaacggcga aacctacgct tgcgcattgt ccaaaattta taccttcggc
      781 ccgactttcc gtgctgaaaa ctccaacacc agccgtcacc tggcggaatt ctggatgctg
      841 gagccggaag tggcgtttgc taacctgaac gatattgcgg gtctggctga agccatgctg
      901 aaatatgtct tcaaagcggt tctcgaagaa cgcgctgacg acatgaaatt cttcgctgaa
      961 cgcgtagata aagatgccgt ttcacgtctg gaacgcttca ttgaagccga ttttgcgcag
     1021 gtggattata ccgacgcagt gaccattctc gaaaactgcg gcaggaagtt tgaaaacccg
     1081 gtttactggg gagtcgatct ctcttctgag catgagcgtt atctggcgga agaacacttt
     1141 aaagcaccgg tagtggttaa aaactatccg aaagatatta aagcgttcta tatgcgcctt
     1201 aacgaagacg gtaaaaccgt tgcggctatg gacgttctgg ctccgggcat cggtgagatc
     1261 attggtggct cccagcgtga agaacgtctg gacgtgctgg acgagcgtat gctggaaatg
     1321 ggcctgaata aagaagatta ctggtggtat cgcgatctgc gtcgctacgg tactgttccg
     1381 cattcaggtt tcggtcttgg ttttgaacgt ctgattgctt acgtaactgg cgtgcaaaac
     1441 gtacgtgatg tgattccgtt cccacgtact ccgcgtaacg ccagcttcta atttagcgtc
     1501 ttcaagagcc agcgcccgct ggctcttttt ttatcttttc tgtcaagtta tctgttgtta
     1561 aggtcaagca atctatttgc aaccccgcca taacgatcat cctgttacgg aatattacat
     1621 tgcaacattt acgcgcaaaa actaatccgc attcttattg cggattagtt ttttcttagc
     1681 taatagcaca attttcatac tattttttgg cattctggat gtctgaaaga agattttgtg
     1741 ccaggtcgat aaagtttcca tcagaaacaa aatttccgtt tagttaattt aaatataagg
     1801 gaaatcatat aaatagatta aaattgctgt aaatatcatc acgtctctat ggaaatatga
     1861 cggtgttcac aaagttcctt aaattttact tttggttaca tattttttct ttttggaaac
     1921 caaatcttta tctttgtagc actttcacgg tagcgaaacg ttagtttgaa tggaaagatg
     1981 cctgcagaca cataaagaca ccaaactctc atcaatagtt c
//