From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: H.vulgare Glb 1 gene for 1-3,1-4-beta-D-glucanase
Date: 4 Sep 92 10:02:39 GMT
Distribution: bionet
Checksum: 64119     9

LOCUS       BLYGLB1      4849 bp ds-DNA             PLN       04-SEP-1992
DEFINITION  H.vulgare Glb 1 gene for 1-3,1-4-beta-D-glucanase
ACCESSION   X56775
KEYWORDS    lichenase.
SOURCE      Hordeum vulgare DNA.
  ORGANISM  Hordeum vulgare
            Eukaryota; Plantae; Embryobionta; Magnoliophyta; Liliopsida;
            Commelinidae; Cyperales; Poaceae.
REFERENCE   1  (bases 1 to 4849)
  AUTHORS   Wolf,N.
  TITLE     Structure of the genes encoding Hordeum vulgare (L3,
            L4)-beta-glucanase isoenzymes I and II and functional analysis of
            their promoters in barley aleurone protoplasts
  JOURNAL   Mol. Gen. Genet. 234, 33-42 (1992)
  STANDARD  full automatic
COMMENT     *source: strain=cv. Northrup King 1558;
            From EMBL    entry HVGLB1;  dated 05-AUG-1992.
FEATURES             Location/Qualifiers
     exon            <955..1027
                     /number=1
     prim_transcript <955..4802
     CDS             join(955..1027,3533..4464)
                     /codon_start=1
                     /translation="MAGQGVASMLALALLLGAFASIPQSVESIGVCYGMSANNLPAAS
                     TVVNMFKSNGINSMRLYAPDQAALQAVGGTGVNVVVGAPNDVLSNLAASPAAAASWVR
                     SNIQAYPKVSFRYVCVGNEVAGGATQNLVPAMKNVQGALASAGLGHIKVTTSVSQAIL
                     GVYSPPSAGSFTGEADAFMGPVVQFLARTGAPLMANIYPYLAWAYNPSAMDMSYALFT
                     ASGTVVQDGSYGYQNLFDTTVDAFYTAMAKHGGSNVKLVVSESGWPSAGGTAATPANA
                     RIYNQYLINHVGRGTPRHPGAIETYVFSMFNENQKDNGVEQNWGLFYPNMQHVYPISF
                     "
     mat_peptide     join(1015..1027,3533..4461)
                     /EC_number="3.2.1.73"
                     /note="1-3,1-4-beta-D-glucanase"
                     /product="lichenase"
                     /codon_start=1
     TATA_signal     832..838
     sig_peptide     955..1014
     intron          1028..3532
                     /number=1
     exon            3533..4802
                     /number=2
     polyA_site      4795..4799
     polyA_site      4798..4802
BASE COUNT     1260 a   1217 c   1119 g   1253 t
ORIGIN
        1 tttgacaagt ataaagacat gcttaactaa agtggtttga gtaattctat gattctttga
       61 caaaaatctt aattaattag gtacaccaaa aacacaaatg ttcttatgat aaacaaaatt
      121 gttggtttct tgcaatataa taacaaaaca atagcatgta aaaatacgag catgtactat
      181 atatataccg gtatgagacg gtaaaaggta gagagaccaa aggcttttga tgtactccaa
      241 atctctttaa attgacccca aatatccatt actaaaatat aatttcattt gtatacatgt
      301 cacttcacga taagaaacaa caaagccgac aaaaccgttg cgcccagttc tttcctacgt
      361 aagtcatgtc aacttcaaag ataaaaaaaa aatcatcgcc aacaagtctc cgttgtgtca
      421 attcttctta cgtaagttat gtcggattcc accgaacacg gtccttgcgt gaaaaaaccg
      481 ccgccgaatg tcgttgagta agacgtgaac atatccgacg cggcgcgacc catcattgac
      541 ctagaaactt cactttcatg gtacatcatg ggtggagtcc aaaattcaaa ctattttttc
      601 aaaagttgtt tggtaccact atgaatgaat gaattatccc cttcccctac ctgcaacaac
      661 aacctggtgt accggataac ctctgcccac caccagacac acactgtgag aaggcggtga
      721 cgcatgcaaa ccggcctagg tagtcaatcg caacaggcta aataaatgtc gctggaggcg
      781 ttgggcctgc gctcccgagt ggattggacc gaactatgtc tcctcggatc ctatataagg
      841 ggcctgcacc ccgttgtggc ctcaccagaa aagaaacaac aagagcttta cagagagcct
      901 tggcatcacc cacccacacc ctcaccctcc aacgcagcta gagagagaaa gagaatggca
      961 ggccaaggcg ttgcctccat gttggctctg gcattgctcc tcggagcctt cgcctccatc
     1021 ccacaaagtg attccccttc cttccctccc tctctctctc ttgaagtgat tgggtgcagt
     1081 tttttttttt catgcaggga gtttctttga agatagtaat acgtacgttg atcttagctt
     1141 tcattaagag aagcattagg gagctagcta ggtagccggc ggccatggtg tacccatgct
     1201 cacataaacc tctgtgggac gtcacatagc ataccttcct ctgtttaggt gcatgtcgct
     1261 ttcgttctgc gttttattct tcccgcgcca tcagtcgcat ggtaaagcat gaacggccgg
     1321 cgcccgccgg tctcattatt tacctcgatg cgtccatcaa tgcatgggag cacagtaact
     1381 tttcaaaaaa aaaattcgaa aactaaacac ggtaaatttt gtaaaaaaaa tcgttttcac
     1441 tcgctaaaag aatcaggagt cttgggtttt gctgcggagt atacgtatca aaatatatgc
     1501 accgcacgct ccaagataaa agtgcacgtc agcacgtgtg atcggccttc attgtgtttc
     1561 ttctatggaa gaaaacgttg caagaagtag gtattccgca ggaaaattaa tatcttaaga
     1621 acaacaaaaa tgatattttc tagataaaac attgaaagag gaagtaagca gtgaaaacga
     1681 tgcttgtaga tagtttcaac tgttttaacc ataaattaat ctagttgatc gtacgcaaaa
     1741 gcatcaagca aaagcttgca aatgaagtct cgaaatgttt ggtgaccatg catgtcagtt
     1801 agctagctgc atctttttac aaagtcagaa ggtttaacta acgacctaca agtagctagc
     1861 taggccagtg tctctttctc cagtgtcatg catgctaatc acctagaaag tttttcttct
     1921 tcttcctcct cttccctgca tgcaaagctg tgcatgcatg tctcgacagt gtcgccctag
     1981 cactgtagac acgagcccta ccacttaatt ggtggtttgt tccctgatta attcggtgca
     2041 actactgctg aaccacccgt cgctaaccat ctttttcttc ttcctttggt ccgcatcttg
     2101 acgttttttc agcgatggac gatcggtacc ggccagtcca gtggtcctac tcgtttcagc
     2161 tagccactgt tccattcttt taagcttaac gaagacgatt acaccagctg ctagctagcc
     2221 agtaactata actcaactgc agcctttgtg tagtcatcat atcgactttg aaagtgcaac
     2281 cggtggatcc atggtacatg acgccatcga tcaccaggca ctaactaacc atgcataacc
     2341 caggtgttgg ttctgctggc ctttccaagt ttggtctgcc tgaaaagaat tgtgatataa
     2401 taggattgta ggttagtgta gtggtactag ctagtagttg gcacttgatc ggccgggcag
     2461 caagttaaga agaggattaa gttgcgtgta cttatatgga gtactttgtc atgcacgtga
     2521 gctagaccgt ttattggagc ttagctggga gcagccggag gcatgcatgc acgccatggc
     2581 gatccacatc gatcgtatgt ggactatcca acggccgtgc tgcggacgtt ggaccagaaa
     2641 ctttggtttg tcctagcatg tatgtatgca tgaggtctct acacccccct gtatttctag
     2701 gcttcctgcc aattgccatc tacgtgtggt cgacctccat tctgccccta cgatattcct
     2761 ggcccgttta gtttaataaa tccagctagc tagtgccgtg caagtactac acaatttatg
     2821 ccataccatt gattggcgat ggcactctta agtgtacacg tactgaaaaa tacatgtatt
     2881 ctttctagca taattgatgt accaactatc aagtgtaaca ctttaataga gtagattggt
     2941 gtagagttag ttggtaaacc aagcaaatca gggagggtaa aagattcact gcactggatg
     3001 ctagtcaaag ttcattcctt cctttaaggt tatctagttc ctttttctcc ttgctcgtgg
     3061 tagagtagct agtagctagt gacaagtcgg tcaaggcgcc ggccgtgaaa atagcaatgt
     3121 tcctcggccg tgtgcgtgca tctgacacca actcgtgact gtaaaaaaaa caatatttaa
     3181 gtggtgcatc agccaccaaa ctatatagag agaaagagat aaaaaaatgt gcaaaactag
     3241 ttagctagag gcttacacat atgcatccat gcatgctaca acatttcagt taaacgtcct
     3301 tggcggggag ttagtattat ccctcgccaa caggtcaaaa ggctctgtgt gcatgtgtgt
     3361 tcatgcatcg ccgccattct tgcttattgg tttctttttt atattccatc acatgccatc
     3421 atgggataat tttttaattt tctactatgg catggaacag tgctactact ctacctggtg
     3481 caaataattg attttgtgaa ggttaactaa ccgaggttat attacattgc aggcgtggag
     3541 tccatcgggg tgtgctacgg catgagcgcc aacaatctgc cggcggcgag caccgtggtc
     3601 aacatgttca agtccaacgg gatcaactcc atgcggctgt acgctcccga ccaggcggcg
     3661 ctgcaggcgg tcggcggcac gggcgtgaac gttgttgtgg gcgcgcccaa cgacgtgctc
     3721 tccaacctcg ccgccagtcc cgcagcggct gcatcgtggg tgaggagcaa catccaggcg
     3781 taccccaagg tctccttccg gtatgtctgc gtgggcaacg aggtcgccgg cggcgccacc
     3841 cagaaccttg tccccgccat gaagaacgtg cagggcgcgc tggcctccgc cgggctgggc
     3901 cacatcaagg tgaccacgtc ggtgtcgcag gccatcctgg gggtgtacag cccgccgtcc
     3961 gccgggtcct tcaccggaga ggcggacgcg ttcatgggcc ccgtggtgca gttccttgcc
     4021 cgcaccggcg cgccgctcat ggccaacatc tacccgtacc tggcctgggc ctacaacccg
     4081 agcgccatgg acatgagcta cgcgctcttc accgcctccg gcaccgtggt ccaggacggc
     4141 tcctacgggt accagaacct gttcgacacc accgtggacg ccttctacac ggccatggcc
     4201 aagcacggcg gctccaacgt gaagctggtg gtgtccgaga gcgggtggcc gtcagccggc
     4261 ggcacggcgg cgaccccggc caacgccagg atctacaacc agtacctcat caaccacgtc
     4321 gggcgcggca ccccccgcca cccgggcgcc atcgagacct acgtcttctc catgttcaac
     4381 gagaaccaga aggacaacgg cgtggagcag aactgggggc tcttctaccc caacatgcag
     4441 cacgtctacc ccatcagctt ctgatgaggt agcagctacc tagtgcccgt atgtccgtac
     4501 gtacgcgcgc gcgtataaga gcgtgtacgc cgtacgtatg cgcacattat gtattgtaca
     4561 gggcttgggt tgggaacttg ggatgcgacc gctgaggcag ctcagatcgt acgcgagtag
     4621 tggcttgcta tactagtgta ccagtacgta tgattttcga tggaagggaa gcatatgcaa
     4681 acgctccccc ttcctcgatt gatcatgcac ttgatacgta cacgcatgtg tgcgtaccta
     4741 ggaactatat tgtagggttc aaaatttcgt caaaacttga cgaaatttgt ccaaaataat
     4801 aaagtatgaa atatatcggc gaagaccgaa tattcctata tataatttt
//