From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: M. olivasterospora fms14 gene for formimidoyl fortimicin A
Date: 12 Jan 93 05:48:30 GMT
Distribution: bionet
Checksum: 11951 5

LOCUS       MPUFMS14     1896 bp ds-DNA             BCT       12-JAN-1993
DEFINITION  M. olivasterospora fms14 gene for formimidoyl fortimicin A
            synthetase.
ACCESSION   D10050
KEYWORDS    FAD containing; fms14 ing; formimdoyl fortimicin A synthetase.
SOURCE      Micromonospora olivasterospora DNA.
  ORGANISM  Micromonospora olivasterospora
            Prokaryotae; Bacteria; Firmicutes; Micromonosporaceae.
REFERENCE   1  (bases 1 to 1896)
  AUTHORS   Dairi,T., Yamaguchi,K. and Hasegawa,M.
  TITLE     N-formimidoyl fortimicin a synthase,a unique oxidase involved in
            fortimicin a biosynthesis: purification, characterization and gene
            cloning
  JOURNAL   Mol. Gen. Genet. 236, 49-59 (1992)
  STANDARD  full automatic
COMMENT     Submitted (06-Dec-1991) to DDBJ by: Dairi Tohru Tokyo Research
            Laboratories Kyowa Hakko Kogyo Co.,Ltd 3-6-6 Asahi-machi
            Machida-chi Tokyo 194 Japan Phone:  0427-25 2555 x272 Fax:
            0427-26-8330.
FEATURES             Location/Qualifiers
     misc_binding    340..357
                     /bound_moiety="putative FAD binding region"
     CDS             <301..1749
                     /gene="fms14"
                     /product="formimidoyl fortimicin A synthetase"
                     /codon_start=1
                     /translation="VVDAAPNYDVVVAGNGALGLSLGLVLARRGLRVAVVGRADRPYA
                     ASAAAGAMNGCFGEVTPHLLRSEHGRLKLAMDYQATGRWDAWEQGLLEESDETAIRTA
                     NGTVVILNTVGVHEIDTEGFRAIRAALDEYKEPYEDLDPAEVPWLHPAPTARPLHAMF
                     IPGEHAVDAPALLRALSAAFTRNGGTLVDDLVGEVLLVGGRAVGVRLTSGTTLSAGHV
                     ALATGAATTKLLDCLPEDVRHRIPAIVAGRGVALMVRTTDGVVPESVIRTPNRAFACG
                     LHVVPRRDGLIYLGATNEVESQLGTTAAIGELNLLLSGTSQMHDDLVNAWVERIMVGN
                     RPVPLDGFPLLGSVGVEGLWLMTGTYRDGLHQSPLLAADMAARILGEPHDSELDVFTP
                     VRPPIEAMTREESLEMAVRHTLGSGYEHEWRLPDDWPPMIEKHFRAAFGRTLEQVDPE
                     FVPPPEMFIFADESIHAALRTYYEAHRGASGR"
BASE COUNT      288 a    657 c    660 g    291 t
ORIGIN      
        1 tcgattccgc caggaccggt cggacacgct agcgcgttcg tccctccaac ccacggcgaa
       61 ccgccggttt ttgctccatt tgccgccact cgcgtcaatc cgctgcgcaa cccggaagcc
      121 taatcttggg cgcaatccag gggtggttgc gccggttgcg ctggggtagc atccttgccc
      181 agtatcggca tcataattgt tttccgatca ccgccaacgg gttcgatcgc gaacccagga
      241 aaacaactac ggcgccgccg gagttggcac gcaaggtctg cgatcgagat ggtaattgac
      301 gtggttgatg ctgccccaaa ttacgacgtg gtcgtcgccg gtaacggcgc actcgggttg
      361 tcgctcggac tggtcctggc gcggcggggc ctgcgggtgg cggtggtcgg ccgggccgac
      421 cggccctacg ccgcctccgc cgccgccggt gccatgaacg ggtgcttcgg cgaggtcacc
      481 ccgcacctgc tgcgcagcga gcacggccgg ttgaagctgg ccatggacta ccaggcgacc
      541 ggccggtggg acgcctggga gcagggtctg ctggaggagt ccgacgagac cgcgatccgc
      601 acggcgaacg gcaccgtggt catcctgaac accgtcggcg tccacgagat cgacacggag
      661 ggcttccggg ccatccgcgc ggcgctcgac gagtacaagg agccctacga ggacctcgac
      721 ccggccgagg tgccgtggct gcacccggcg ccgaccgccc ggccgctgca cgcgatgttc
      781 atcccgggcg agcacgcggt cgacgccccg gcgctgctcc gggcgctctc cgcggcgttc
      841 acccgcaacg gcggcacgct cgtcgacgac ctcgtcggcg aggtgctgct ggtcggtggt
      901 cgtgccgtcg gcgtacggct caccagcggt acgaccctgt ccgccggcca cgtcgccctg
      961 gcgaccggcg cggccaccac gaagctgctg gactgcctgc ccgaggacgt ccgccaccgg
     1021 atccccgcca tcgtcgctgg ccgcggggtg gccctcatgg tgcgcacgac ggacggcgtc
     1081 gttccggagt cggtgatccg cacccccaac cgggcgttcg cctgcgggct gcacgtggtg
     1141 ccgcgccgcg acgggctgat ctacctgggc gccaccaacg aggtggagtc gcagctcggt
     1201 acgacggccg cgatcggcga gctgaacctg ctgctcagcg gcaccagcca gatgcacgac
     1261 gacctggtga acgcctgggt ggagcggatc atggtgggca accgtccggt cccgctggac
     1321 ggcttcccgc tgctgggctc ggtcggagtc gagggtctgt ggctcatgac cggcacgtac
     1381 cgcgacggct tgcaccagtc gccgctgctg gccgcggaca tggcggcacg gatcctcggg
     1441 gaaccgcacg acagcgagct ggacgtcttc acgccggtac gcccgccgat cgaggcgatg
     1501 acgcgcgagg agtccctcga gatggccgtg cggcacaccc tgggcagcgg ttacgagcac
     1561 gagtggcggc tgccggacga ctggccgccg atgatcgaga agcacttccg ggccgcgttc
     1621 ggccgaacgc tggaacaggt cgatccggag ttcgtcccgc cgccggagat gttcatcttc
     1681 gccgacgaat ccatccacgc cgcgctgcgc acgtactacg aggcgcaccg gggcgcgtcc
     1741 ggacggtaga gccaatgtgg aaggggttca agtgtgtcga ccaggacgga taccggcgtg
     1801 acacgagtgc tgctgatcaa gccgcccatt cgcgcgtgca tgatcgagat cggccggcac
     1861 atgccgatcg ggctcgccta cctcgccgcc cagctg
//