From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Actinomadura sp. dac gene for DD-peptidase
Date: 22 Jul 92 10:00:57 GMT
Distribution: bionet
Checksum: 11915     5

LOCUS       ATDACG       1698 bp ds-DNA             BCT       22-JUL-1992
DEFINITION  Actinomadura sp. dac gene for DD-peptidase
ACCESSION   X64790
KEYWORDS    D-alanyl-D-alanine carboxypeptidase; dac gene.
SOURCE      Actinomadura sp. DNA.
  ORGANISM  Actinomadura sp.
            Prokaryotae; Firmicutes; Thallobacteria;
            Actinomycetes and related organisms; Maduromycetes.
REFERENCE   1  (bases 1 to 1698)
  AUTHORS   Dusart,J.
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
REFERENCE   2  (bases 1 to 1698)
  AUTHORS   Granier,B., Duez,C., Lepage,S., Englebert,S., Dusart,J.,
            Dideberg,O., Van Beeumen,J., Frere,J.M. and Ghuysen,J.M.
  TITLE     Primary and predicted secondary structures of the Actinomadura R39
            extracellular DD-peptidase, a penicillin-binding protein (PBP)
            related to the Escherichia coli PBP4
  JOURNAL   Biochem. J. 282, 781-788 (1992)
  STANDARD  full automatic
COMMENT     *source: strain=R39;
            From EMBL    entry ADACG;  dated 15-JUN-1992.
FEATURES             Location/Qualifiers
     RBS             34..39
     sig_peptide     44..190
     CDS             44..1660
                     /note="DD-peptidase"
                     /product="D-alanyl-D-alaninecarboxypeptidase"
                     /gene="dac"
                     /codon_start=1
                     /translation="MKQSSPEPLRPRRTGGRGGARRAAALVTIPLLPMTLLGASPALA
                     DASGARLTELREDIDAILEDPALEGAVSGVVVVDTATGEELYSRDGGEQLLPASNMKL
                     FTAAAALEVLGADHSFGTEVAAESAPGRRGEVQDLYLVGRGDPTLSAEDLDAMAAEVA
                     ASGVRTVRGDLYADDTWFDSERLVDDWWPEDEPYAYSAQISALTVAHGERFDTGVTEV
                     SVTPAAEGEPADVDLGAAEGYAELDNRAVTGAAGSANTLVIDRPVGTNTIAVTGSLPA
                     DAAPVTALRTVDEPAALAGHLFEEALESNGVTVKGDVGLGGVPADWQDAEVLADHTSA
                     ELSEILVPFMKFSNNGHAEMLVKSIGQETAGAGTWDAGLVGVEEALSGLGVDTAGLVL
                     NDGSGLSRGNLVTADTVVDLLGQAGSAPWAQTWSASLPVAGESDPFVGGTLANRMRGT
                     AAEGVVEAKTGTMSGVSALSGYVPGPEGELAFSIVNNGHSGPAPLAVQDAIAVRLAEY
                     AGHQAPEGARMMRGPVQGSGELECSWVQAC"
     mat_peptide     191..1657
BASE COUNT      227 a    637 c    629 g    205 t
ORIGIN
        1 cctgggctag ggtgcgttgc ttccgccccc ttcgaggaga cccatgaagc aatcctcccc
       61 cgaacccctg cgcccccgcc gcaccggagg gcgcggcggc gcccggaggg ccgccgccct
      121 cgtcacgatc cccctgctgc cgatgacgct cctgggagcg tcccccgcgc tcgccgacgc
      181 ctccggagcc cgcctgaccg aactgcgcga ggacatcgac gccatcctgg aggaccccgc
      241 actggagggc gccgtgtcgg gggtggtcgt cgtggacacc gcgaccggcg aggagctgta
      301 ctcgcgcgac ggcggcgagc agctgctgcc cgcctccaac atgaagctgt tcaccgcggc
      361 cgccgccctg gaggtcctgg gcgccgacca ctccttcggg accgaggtcg cggccgagtc
      421 cgctcccggg cgccggggag aggtgcagga cctctacctg gtgggccggg gcgacccgac
      481 gctctccgcc gaggacctgg acgccatggc cgccgaggtc gcggcctccg gggtccgcac
      541 ggtcaggggc gacctgtacg ccgacgacac gtggttcgac tccgagcggc tcgtggacga
      601 ctggtggccc gaggacgagc cctacgccta ctcggcccag atctcggccc tgacggtcgc
      661 ccacggggag cgcttcgaca ccggcgtgac ggaggtctcg gtgacccccg cggcggaggg
      721 cgagcccgcc gacgtggacc tcggcgccgc ggagggctac gccgagctcg acaaccgggc
      781 cgtcaccggc gccgccggca gcgccaacac cctcgtcatc gaccgcccgg tgggcaccaa
      841 caccatcgcg gtcaccggct cgctccccgc ggacgccgca cccgtgaccg cgctgcggac
      901 ggtcgacgag cccgccgcgc tcgcgggcca cctcttcgag gaggcgctgg agagcaacgg
      961 cgtcacggtg aagggcgacg tcggcctggg cggtgtcccc gccgactggc aggacgccga
     1021 ggtgctcgcc gaccacacgt cggccgagct ctccgagatc ctcgtgccct tcatgaagtt
     1081 cagcaacaac gggcacgccg agatgctggt caagagcatc ggccaggaga ccgccggcgc
     1141 gggcacctgg gacgccgggc tcgtcggcgt ggaggaagcg ctgtccggcc tgggcgtgga
     1201 caccgccggc ctggtcctca acgacggctc cggcctgtcg cggggcaacc tggtcaccgc
     1261 ggacaccgtc gtcgacctgc tcgggcaggc gggttccgcc ccctgggcgc agacctggtc
     1321 cgcctcgctg ccggtcgcgg gcgagagcga cccgttcgtc ggcggcaccc tcgccaaccg
     1381 gatgcgcggt accgccgccg agggcgtggt cgaggccaag accgggacga tgagcggggt
     1441 cagcgccctc tccgggtacg tgcccgggcc ggagggcgag ctggcgttca gcatcgtgaa
     1501 caacggccac tccggtcccg cgcccctcgc ggtgcaggac gcgatcgcgg tgcgcctggc
     1561 cgagtacgcg ggccaccagg cgccggaggg cgccaggatg atgcgcggcc cggtccaggg
     1621 cagcggcgag ctggagtgct cctgggtgca ggcctgctga ccgggaggag tacctggcgt
     1681 ccggcggtgg cccgaggg
//