From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: P3A2=DNA-binding protein [Strongylocentrotus purpuratus=sea
Date: 5 Nov 92 23:01:38 GMT
Distribution: bionet
Checksum: 07207 7

LOCUS       S77941       3660 bp    mRNA            NLM       21-OCT-1992
DEFINITION  P3A2=DNA-binding protein [Strongylocentrotus purpuratus=sea
            urchins, embryo, mRNA, 3660 nt]
ACCESSION   S77941 X57014
KEYWORDS    .
SOURCE      sea urchins embryo
  ORGANISM  Strongylocentrotus purpuratus
            Unclassified.
REFERENCE   1  (bases 1 to 3660)
  AUTHORS   Calzone,F.J., Hoog,C., Teplow,D.B., Cutting,A.E., Zeller,R.W.,
            Britten,R.J. and Davidson,E.H.
  TITLE     Gene regulatory factors of the sea urchin embryo. I. Purification
            by affinity chromatography and cloning of P3A2, a novel DNA-binding
            protein.
  JOURNAL   Development 112, 335-350 (1991)
  STANDARD  full automatic
COMMENT     This entry incorporates information provided by the National
            Library of Medicine's GenInfo Backbone as NCBI gibbsq 77941.
            This sequence comes from fig4.
FEATURES             Location/Qualifiers
     CDS             80..1459
                     /note="DNA-binding protein;  For the protein sequence
                     (NCBI gibbsq 77942):
                     Method: conceptual translation with partial peptide
                     sequencing.
                     This sequence comes from fig4."
                     /product="P3A2"
                     /codon_start=1
                     /translation="MMISEDISEPSSPDTPFDDSDLLNSSMTDDVSAQLAASGPIGVR
                     AAAAIATGKKRKRPHSFETNPSIRRRQQTRLIRKLKATIDEYATRVGQQAVVLTCTPG
                     KHDGNFKVFGAAPLENIMRNLKGIVLQDLDNALAQRAPQPSNENSDLYELPPLVIDGI
                     PTSVNKMTQAQLRAFIPLMLKYSTGRGKPGWGKESCRPVWWPSDLPWANVRSDVRSED
                     EKRKVSWTHALVTIVINCYKHHGRDDLLPEFIEDKCKEIEASQNQVASLPTATLLPSH
                     AVVHTINNPDGTVSLIQVDTGATVATLADVTQVQQLTNLQTLQQVRLQPLQIQHALGN
                     QQAEATQAVQTLAEVAAAQGGDGELTEGQTVTTLPEGTQLVLASDGSLQAINDGTAQG
                     IVIPASVYQTVVAGDGQPIQIANVNIAQQSGGGTTMAAIKNAVMQSQPIPSQVATLVV
                     NAASHDQHT"
BASE COUNT     1071 a    706 c    726 g   1157 t
ORIGIN      
        1 cgcatgtgaa aatcatcaca gaggacaaat atttggaccg tttttctcgc tattcatttg
       61 gaggatataa aaactcaata tgatgatcag tgaagatatc agtgagccgt cctccccgga
      121 cacgccgttt gatgattcag acttgcttaa ttcaagtatg acggatgatg tctctgcaca
      181 attagcagca tctggtccta taggcgtacg tgctgctgca gccatagcaa cagggaagaa
      241 acgcaagaga ccgcattctt ttgagacaaa tccttccatt cggcgtcgcc aacaaacaag
      301 attgatcagg aagctgaaag caaccattga tgagtatgca accagagtag ggcagcaagc
      361 tgtcgttcta acctgtactc ctggcaaaca tgatggcaat ttcaaagtat ttggtgctgc
      421 tcccctggaa aatattatga gaaacttgaa gggaatagtc ctacaagacc ttgacaatgc
      481 cttggcacag agagcccccc agccgtctaa tgagaattca gacctgtatg agctacctcc
      541 tctggtcatt gatggcattc ctacatcggt caacaagatg acccaagccc agctaagggc
      601 atttatacct ctcatgctta agtactcaac gggaagaggg aagccaggat ggggaaagga
      661 gagctgcagg ccagtgtggt ggccatctga cctgccatgg gctaatgtga gaagtgatgt
      721 cagatcagaa gatgaaaaga gaaaggtttc atggacccac gctttggtca cgattgtcat
      781 caactgctac aagcatcatg gaagggatga cctcttgcca gagtttattg aagataagtg
      841 caaggagata gaggcctctc aaaaccaggt tgcttcacta ccgacagcaa ccctactacc
      901 atcccatgct gtggtacaca caatcaacaa ccctgatggg acagtctctc ttatccaggt
      961 tgatacagga gctactgtag caaccctggc ggatgtcacg caagtacaac agttaaccaa
     1021 tcttcagact cttcagcaag taagacttca gcctctgcag attcagcatg cactaggcaa
     1081 tcagcaagca gaggctacac aagctgtaca gactctggct gaggtagctg ctgcacaagg
     1141 aggagatggc gagttgactg aagggcaaac agtaaccaca ttgccagagg gcactcaact
     1201 tgttctagca agcgatggat cactccaagc tataaatgat ggcacagcac aaggtattgt
     1261 tatcccggct tcggtgtacc agacagttgt agcaggagat ggacagccca ttcagatcgc
     1321 aaatgtgaat attgctcagc agagtggcgg aggaaccact atggcagcta ttaagaatgc
     1381 tgtcatgcaa tcccagccaa tcccttctca agtagctact ctagtagtca atgcagcatc
     1441 acatgatcaa catacatagc accaatcatc aacacattgc ccttctcaag gatggtggtc
     1501 ttgagctttc ccacaggccc aggacaagat ttcaggaaaa tggtatgcac aataatttgt
     1561 tgttgagaga ttgcatgggt tcattgctca ttcataacta tatcttttcc ttgtctcata
     1621 aatacttgtg atcaattcaa atcatgcaaa acttgatatt aatagcaagc atgtactaaa
     1681 gagatggaag tttgtaagtc tatgattgat tggaggaatt gcaatcaatc cacatcaatt
     1741 catctctttg taagcagggc ccatacacct aacagattgt cattgcagat agaaacatac
     1801 agtcagctgg cctgacttgt tagtttcttt tcttttatct ttttttctgt ttgttttgtt
     1861 ttatgttttt gttcttattc atttcagtta gcttttgagg gtcgtgaaat taatatgaca
     1921 tctcctttat ttcaaaattt ctcttgctga tattgaaaga gaggattggg ttcttgcata
     1981 ggagaaatat ttgatcatcc ccatgctgtc cagcttgttg aattttgttc tgtttagagc
     2041 atgtgttgta cttggttttc caaaacagga acacattctt tttatattga ggtaatgaat
     2101 attgttatat cagaacctta gtcttaaact ttgcaggtgc tgttagcagt tgagaaaatc
     2161 cacattaaac atcattttat tatgtgtgag ggaaccgata atataacatt ttgtggctat
     2221 ctgtgtgtga ttcaatgtta caaatattgg tgtagattgc taacaggaaa ggtgcttaac
     2281 tactgtaaaa ttcaaacttg cattaagcat tttagggacc cattcacagg aagcatggac
     2341 agaatgtata agtacgtttt gatggtgagg gtatataatt aatgtccttc tcataacatg
     2401 cactcctctt tatttcaaat tttggttgcc aacatgatga gctggttgtc tagttcacaa
     2461 cattcaactc acttcaatat cagtatttgt tttgcaaaat ttgtaatttc ccatccaggc
     2521 catgtttttt taataagggt gtctggtagg atttgtagtt gttcaatagc atcatgtaag
     2581 cacacaaatt atattttctt gcccacacat gtctattgtt tagggtgcag tcactattct
     2641 taattttttt tttctatcat cagggttggg ataaaactct attctcccta cttgatcagg
     2701 atgtattctg aattcacctt ttttaaatgc acaacaagca ctttgtcaca atgtttgtgc
     2761 atgttaaagc aaatgaatgc atggagaagt tattttttta aatccccccc ccccccatct
     2821 tttccatgat aggtagaaca gtaagactca agaatcttac taaaatggac atatccatta
     2881 tcaagctttg catgaattcc ctttgaattt cttgtcgtta tcatttttgt ggtattattg
     2941 ttgaaagagg ggaattcggt cagcaagtac acagttaatt gttcattttg ttagacttgc
     3001 atttggctac ttacatgtga agttcttcta cctcacttgt ttgcatgtat gtaagatgct
     3061 gattgaaatt caacagtact atggactgtt taactcgatg cttaccaaag agcattgtac
     3121 acttcatgta gtagcctata gcaaaggtga cagggaacaa gtgatttttc tcaatggatt
     3181 cccatgttca agattgaaat gttaaaccat acagttttat tttttgtcca tcctgcccat
     3241 gtgtcaaaaa tgtagagtgt tataagttta tgacactctt cccattttca agtttcttcc
     3301 ataattgttg tcatttcatg tttttagata tgaatatttt tgaagcagat tgacatacat
     3361 ctaaaggttt aaatcatgct taattaaaca tttgcaacat attcatctat tgattgaatt
     3421 gcaatgactt ttgttttatt aatttcaatt taaacttaaa acttatgaag tatatttatt
     3481 tgagccttgg taaatttaag tgtaaataac tatgaaatat gtatgatatc agagttattt
     3541 tctattggta aattcatgat atgcctgatc ccgctttgta caggtataga agttttaaac
     3601 attgtacagt gctacatgta ttgcttttat atgcacagta aattttgtca aaccgaattc
//