From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Anabaena sp. nucA gene for nuclease
Date: 21 Dec 92 20:25:50 GMT
Distribution: bionet
Checksum: 41857 4

LOCUS       ANAPNUCA     1318 bp ds-DNA             BCT       17-NOV-1992
DEFINITION  Anabaena sp. nucA gene for nuclease
ACCESSION   X64706
KEYWORDS    DNase activity; RNase activity; endonuclease;
            extracellular nuclease; nucA gene.
SOURCE      Anabaena sp. DNA.
  ORGANISM  Anabaena sp.
            Prokaryotae; Gracilicutes; Oxyphotobacteria; Cyanobacteria;
            Nostocales; Nostocaceae.
REFERENCE   1  (bases 1 to 1318)
  AUTHORS   Muro-Pastor,A.M., Flores,E., Herrero,A. and Wolk,C.P.
  TITLE     Identification, genetic analysis and characterization of a
            sugar-non-specific nuclease from the cyanobacterium Anabaena sp.
            PCC 7120
  JOURNAL   Mol. Microbiol. 6, 3021-3030 (1992)
  STANDARD  full automatic
COMMENT     *source: strain=PCC 7120; *source: clone_library=wild-type bank in
            cosmid vector pRL25C; *source: clone=35G5;
               From EMBL    entry ASPNUCA;  dated 10-NOV-1992.
FEATURES             Location/Qualifiers
     RBS             431..437
     CDS             447..1271
                     /gene="nucA"
                     /product="NucA"
                     /codon_start=1
                     /translation="MGICGKLGVAALVALIVGCSPVQSQVPPLTELSPSISVHLLLGN
                     PSGATPTKLTPDNYLMVKNQYALSYNNSKGTANWVAWQLNSSWLGNAERQDNFRPDKT
                     LPAGWVRVTPSMYSGSGYDRGHIAPSADRTKTTEDNAATFLMTNMMPQTPDNNRNTWG
                     NLEDYCRELVSQGKELYIVAGPNGSLGKPLKGKVTVPKSTWKIVVVLDSPGSGLEGIT
                     ANTRVIAVNIPNDPELNNDWRAYKVSVDELESLTGYDFLSNVSPNIQTSIESKVDN"
BASE COUNT      387 a    262 c    325 g    344 t
ORIGIN      
        1 tagcaatttt tcttactatt tcttactata tggcacactg agatgaacgc aggagggcga
       61 ttgcattgct tttgacaaca agcagccgca tggtattgaa cgggtggtgg gtgataggtg
      121 gtgtgtgtgt ttctttagac tcaaggggga gtgtttggag caggaatacg ggaagcaatt
      181 gagtttggtt tagcccttcc gccaaattca agcgttcgct cccgcctctc gcagagagga
      241 aaagtaagcg atgacctacg ctacaccgaa agagcgatgg cgtaacgcaa gagcgtagct
      301 gatcgtcagc aaccctaatt tttttagagg aaaaattagg gttgatcggg caggagactt
      361 gaaaatttga agcctagctg agtgaaagtc aggaaataca atcacaagtg ttgaagtgaa
      421 ataaatctct aaaggtgcgt caatttatgg gaatttgtgg aaaattgggt gtagcggcgt
      481 tggtggcgtt gattgtcggg tgttcgcctg tccaatccca agtgccacca ttaactgaac
      541 tttccccatc aatcagcgtg catttactgc tgggaaatcc cagtggtgca acgccaacaa
      601 agcttacacc tgataattac ctgatggtca aaaatcaata tgcactctcc tacaacaaca
      661 gcaagggaac tgctaactgg gtagcttggc agcttaactc ctcatggcta gggaacgcag
      721 agcgtcaaga taacttccgc ccagacaaaa cattgcctgc gggttgggtg cgagtgactc
      781 cttctatgta ctctgggagt ggttatgacc gggggcatat tgcaccttca gcagaccgca
      841 ccaagacaac agaagataat gcggctactt tcctgatgac aaacatgatg ccccaaacac
      901 ccgataacaa tagaaatacg tggggaaatt tagaagatta ttgtcgagaa ttagtcagtc
      961 agggtaaaga gctttacatt gttgccgggc ctaatggtag tcttggcaaa cccctcaaag
     1021 gtaaggtgac agttcccaaa tccacttgga agattgttgt cgtactagat agcccaggct
     1081 cagggcttga aggtattact gctaatactc gcgttatcgc agtaaatatt cccaacgacc
     1141 cagaattaaa taatgactgg agggcttata aagtcagtgt tgatgaatta gaaagtttga
     1201 cgggttatga ttttttgtct aatgtttccc ccaatattca aacaagtatt gagagtaaag
     1261 ttgataatta gtaaataggt caagtttcca caactttagt agaaatacca gctaaatt
//