From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: H.vulgare gene for bifunctional alpha-amylase/subtilisin inhibitor.
Date: 25 Jul 92 10:02:39 GMT
Distribution: bionet
Checksum: 21187     4

LOCUS       BLYBAASI      633 bp ds-DNA             PLN       25-JUL-1992
DEFINITION  H.vulgare gene for bifunctional alpha-amylase/subtilisin inhibitor.
ACCESSION   Z12961
KEYWORDS    alpha-amylase/subtilisin inhibitor.
SOURCE      Hordeum vulgare DNA.
  ORGANISM  Hordeum vulgare
            Eukaryota; Plantae; Embryobionta; Magnoliophyta; Liliopsida;
            Commelinidae; Cyperales; Poaceae.
REFERENCE   1  (bases 1 to 633)
  AUTHORS   Leah,R. and Mundy,J.
  TITLE     The bifunctional alpha-amylase/subtilisin inhibitor of barley:
            nucleotide sequence and patterns of seed-specific expression
  JOURNAL   Plant Mol. Biol. 12, 673-682 (1989)
  STANDARD  full automatic
REFERENCE   2  (bases 1 to 633)
  AUTHORS   McKinnon,G.E. and Henry,R.J.
  TITLE     Genomic sequence of bifunctional alpha-amylase/subtilisin inhibitor
            from barley
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
REFERENCE   3  (bases 1 to 633)
  AUTHORS   McKinnon,G.E.
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
COMMENT     *source: strain=Betzes; *source: clone=p3h; *source: chromosome=2H;
            *source: is_macronuclear=N; *source: is_proviral=N; *source:
            is_germline=N;
            From EMBL    entry HVBAASI;  dated 27-JUN-1992.
FEATURES             Location/Qualifiers
     CDS             10..624
                     /product="bifunctional alpha-amylase/subtilisininhibitor"
                     /codon_start=1
                     /translation="MGSRRAGLLLLSLILASTALSRSADPPPVHDTDGHELRADANYY
                     VLSANRAHGGGLTMAPGHGRHCPLFVSQDPNGQHDGFPVRITPYGVAPSDKIIRLSTD
                     VRISFRAYTTCLQSTEWHIDSELAAGRRHVITGPVKDPSPSGRENAFRIEKYSGAEVH
                     EYKLMSCGDWCQDLGVFRDLKGGAWFLGATEPYHVVVFKKAPPA"
     sig_peptide     10..78
                     /note="Differs from previously predicted signalpeptide
                     (Leah, R and Mundy,J (1989): Plant MolBiol 12: 673-682)"
                     /citation=[3]
                     /citation=[1]
     mat_peptide     79..621
                     /note="no introns"
                     /product="bifunctional alpha-amylase/subtilisininhibitor"
                     /citation=[3]
                     /citation=[1]
BASE COUNT      101 a    232 c    203 g     97 t
ORIGIN
        1 gtttcagtca tgggtagccg ccgtgcaggc ctcctcctcc tctcccttat tctggccagc
       61 accgccctct cgcgcagcgc cgatccgccg ccggtgcacg acacggacgg ccacgagctg
      121 cgcgccgacg ccaactacta cgtcctctcg gccaaccgcg cccacggcgg ggggctgacg
      181 atggcgcccg gccacgggcg ccactgcccg ctcttcgtgt cgcaggaccc caacgggcag
      241 cacgacgggt tccccgtgcg catcaccccg tacggcgtcg cgccgtccga caagatcatc
      301 cggctgtcga ccgacgtccg catctccttc cgcgcctaca cgacgtgtct gcagtccact
      361 gagtggcaca tcgacagcga gctggcggcg ggccgccggc acgtgatcac cggcccggtc
      421 aaggacccga gcccgagcgg cagggagaac gccttccgca tcgagaagta cagcggcgcc
      481 gaggtgcacg agtacaagct gatgtcgtgc ggggactggt gccaggacct cggcgtgttc
      541 agggacctca agggtggggc gtggttcttg ggcgccaccg agccatacca tgtcgtcgtg
      601 ttcaagaagg cgccgcccgc ttaaggtcca atg
//