HDTenEightyP/NetNews huggingface dataset:
From: kristoff@genbank.bio.net (David Kristofferson)
Newsgroups: bionet.announce
Subject: CALL FOR VOTES: bionet.plants.maize
Date: 2 Sep 92 11:54:06 GMT
Reply-To: biovote@genbank.bio.net

Voting is now open on bionet.plants.maize.  I have received from Allen
Wright a revised proposal for bionet.plants.maize (note the name
change from the original bionet.maize) which is provided below.  There
were several postings on BIOFORUM/bionet.general about the benefits of
a separate maize group but also many comments about first building up
a maize discussion in the existing bionet.plants newsgroup.  The
parties on opposite sides of the separate newsgroup issue did not
compromise.  I suggest that you read those messages, if you haven't
already, before voting.  Those who favor the creation of the maize
newsgroup should obviously vote YES while those who think that it is
premature and want discussion to begin first on bionet.plants should
vote NO.

----------------------------------------------------------------------
PROPOSAL TO ESTABLISH bionet.plants.maize

Discussion leader: Allen Wright (adwright@iastate.edu)

Purpose: Discussion/inquiries regarding the anatomy, breeding,
genetics, biochemistry, molecular biology, evolution, ethnobotany, and
utilization of maize and its related species, including information
exchange regarding mutants, genetic stocks, mapping and methodology.
------------------------------------------------------------------

Voting is now open on bionet.plants.maize and will run through 24:00
hrs Pacific Time on 1 October 1992.  Please send your vote to any of
the following addresses (the IRLEARN address *is* functional):

Address                               Location        Network
-------                               --------        -------
biovote@daresbury.ac.uk               U.K.            JANET
biovote@genbank.bio.net               U.S.A.          Internet/BITNET
biovote@irlearn.ucd.ie                Ireland         EARN/BITNET

Your vote should contain a single line:

YES on bionet.plants.maize

if you favor creation of the newsgroup or

NO on bionet.plants.maize

if you think that the creation of this newsgroup will adversely affect
the BIOSCI system.  If you are simply not interested in participating
in this newsgroup, please don't cast a NO vote, but instead just don't
vote at all.

The newsgroup proposal must receive at least 80 YES votes to pass and
the number of YES votes must be greater than the number of NO votes by
at least 40.  Discussion of the newsgroup proposal is now closed and
we strongly discourage posting any messages in other forums about the
fact that a CALL FOR VOTES has been issued.

				Sincerely,

				Dave Kristofferson
				GenBank Manager

				kristoff@genbank.bio.net
Newsgroups: bionet.neuroscience
From: comai@U.WASHINGTON.EDU (Luca Comai)
Newsgroups: bionet.genome.arabidopsis
Subject: Postdoctoral position
Date: 25 Aug 92 22:19:04 GMT
Distribution: bionet

Dear Network,
please bring the following ad to the attention of suitable candidates.
Luca Comai

Postdoctoral position: chromatin-organizing regions and gene regulation

A POSTDOCTORAL POSITION is available to study chromatin organization and
gene regulation in plants. We have identified DNA regions that may
organize chromatin domains. These elements, isolated from tomato, bind to
chromosomal scaffolds in vitro and, in transgenic plants, affect gene
expression positively or negatively depending on their configuration. The
objectives are to characterize these elements and their function by
physical and genetic studies. We use Arabidopsis as the model system for
transgenic work and genetic analysis. 

Starting date is as soon as possible. Strong background in molecular
biology or genetics is required. Send CV and ask three people to write
letters to: 

Luca Comai,
Botany Department KB-15
University of Washington
Seattle, WA 98195
Phone: 206-543-4841
From: WILKINSONM@SASK.USASK.CA (Muddled)
Newsgroups: bionet.genome.arabidopsis
Subject: ISO Male sterile lines
Date: 27 Aug 92 16:55:00 GMT
Distribution: bionet


Dear Netters, 

	Does anyone have a male sterile line in which sterility is the 
*only* trait segregating?  More specifically, I need a male sterile 
line in the *COL-0 Background* where the lesion causing the sterility 
has no affect on other aspects of plant growth and development.  If 
the basis for the sterility known that would be even better.  If there 
is such a beast, I would appreciate some seed from the line.  I can be 
reached at the E-mail address below, or by snail-mail at:

		Department of Biology
		University of Saskatchewan
		Saskatoon, SK
		Canada S7N 0W0

			Thanks in advance for you time, 

Mark Wilkinson             | Anyone who has to survive |
Office: tel (306) 966 4430 |    on 1800 calories of    |
"WilkinsonM@Sask.USask.CA" | synthetic orange flavoured|
                           | waffle every day DESERVES |
                           |       a little treat      |
From: PLZMLH@VAX.CCC.NOTTINGHAM.AC.UK
Newsgroups: bionet.genome.arabidopsis
Subject: NASC fax number change
Date: 4 Sep 92 15:03:00 GMT
Distribution: bionet
Original-To: ARAB-GEN@UK.AC.DARESBURY


Please note that from September the 7th

The fax number for the Nottingham Arabidopsis Stock Centre will change.

The new FAX number will be 0602 513251
Newsgroups: bionet.genome.arabidopsis
From: bafp3@uk.ac.sussex.central (Neil Butt)
Newsgroups: bionet.genome.arabidopsis
Subject: electroporation
Date: 28 Aug 92 10:39:50 GMT
Distribution: bionet
Original-To: arab-gen@uk.ac.daresbury
X-Mailer: ELM [version 2.3 PL11]

Dear networkers,
We are about to embark on some plant transformation experimentation, using 
electroporation. All the protocols we have seen use 0.4ml cuvettes for 
plant cells. Our group has used electroporation techniques on yeast using 
0.2ml cuvettes. This may seem a petty factor, but we have not performed 
these experiments before and do not want to take any chances, but is it O.K 
to use the smaller sized cuvettes. We intend to do these experiments in both 
Tobacco and Arabidopsis in case the plant species is a factor in the 
protocol.
Hope someone can help
Neil Butt
Biological Sciences
University of Sussex
Falmer
Brighton
East Sussex, BN1 9QG.
U.K
E-mail     bafp3@uk.ac.sussex.central
(from non-UK)   bafp3@central.sussex.ac.uk
From: DELANEYT@A1.CG.COM (Terry Delaney)
Newsgroups: bionet.genome.arabidopsis
Subject: subscription request
Date: 1 Sep 92 02:28:00 GMT
Distribution: bionet

Please subscribe me to ARABIDOPSIS

Also update my mailing address to:

Terrence P. Delaney
Department of Molecular Genetics
CIBA-GEIGY Agricultural Biotechnology
P.O. Box 12257
Research Triangle Park, NC  27709-2257
(919) 541-8577 	
FAX: (919) 451-8557
e-mail: delaneyt@abru.cg.com


Thanks 10^6!

Terry
From: TAGUE@FRODO.MGH.HARVARD.EDU
Newsgroups: bionet.genome.arabidopsis
Subject: Isolation of RNa from seeds
Date: 25 Aug 92 19:47:29 GMT
Distribution: bionet

Does anyone have a reliable method for the isolation of RNA from dry seeds 
and/or yellow siliques. With the standard RNA prep (SDS/LiCl/phenol/chloroform)
some kind of contaminant comes through with the RNA. Procedures for the removal
of polysaccharides (1M NaCl ppt @ 0C, acid ppt) don't remove it. Any hints or
suggestions would be greatly appreciated. Thanks in advance.
-- Brian W. Tague
From: fbekkaoui@pbi.lan.nrc.ca
Newsgroups: bionet.genome.arabidopsis
Subject: Subscription Request
Date: 4 Sep 92 20:45:00 GMT
Distribution: bionet

Please enter a subscription to the Arabidopsis network for
Faouzi BEKKAOUI at the address fbekkaoui@pbi.lan.nrc.ca

My mail address is 
Plant Biotechnology Institute
110 Gymnasium rd.
Saskatoon Sk. S7N 0W9
Canada

Tel. (306) 975 5241
Fax. (306) 975 4839

Thank you.
From: rob_last@QMRELAY.MAIL.CORNELL.EDU ("Rob Last")
Newsgroups: bionet.genome.arabidopsis
Subject: References on Amino Acids,
Date: 3 Sep 92 01:09:19 GMT
Distribution: bionet

REGARDING                References on Amino Acids, Vitamins, etc.
Dear Colleagues,
Alan Rose and I are writing an article for the book ARABIDOPSIS on amino acid,
purine, pyrimidine and vitamin biosynthesis in flowering plants, with an
emphasis on studies in Arabidopsis. We would like to include a discussion of
all relevant research.  Any assistance that you could provide to make this
article as comprehensive and up-to-date as possible would be appreciated. 
Unpublished results in the form of manuscript preprints or personal
communications would be especially useful.  Please also send reprints of any
relevant articles from your laboratory, especially references that might be
difficult to find (including those from conference proceedings, chapters in
specialized books, etc.).  

We are trying to identify all laboratories that are pursuing projects in these
fields.  Please let me know if you are aware of any other researchers who might
have interesting results to report and might not see this correspondance.  I
would appreciate if you would include your fax number and computer mail address
to facilitate future communications.

We are quite excited about having the opportunity to review this topic.  Thank
you very much for your assistance.

Rob Last
Boyce Thompson Institute at Cornell University
Tower Road
Ithaca, NY  14853-1801
Phone: (607) 254-1325
Fax: (607) 254-1242
E-Mail: Rob_Last@QMrelay.mail.cornell.edu
From: Mitch.McGrath@UM.CC.UMICH.EDU
Newsgroups: bionet.genome.arabidopsis
Subject: unsubscribe
Date: 27 Aug 92 13:42:14 GMT
Distribution: bionet

Please unsubscribe me.  I am moving.
From: Mitch.McGrath@UM.CC.UMICH.EDU
Newsgroups: bionet.genome.arabidopsis
Subject: unsubscribe
Date: 29 Aug 92 02:48:36 GMT
Distribution: bionet

please delete me from this network.  I am moving. Thanx
From: bjkang@RKNA50.RIKEN.GO.JP (Bong-Joong Kang)
Newsgroups: bionet.genome.arabidopsis
Subject: Notifying address and Subscribing
Date: 26 Aug 92 04:35:47 GMT
Distribution: bionet


I just opened a e-mail account as a bjkang@rkna50.riken.go.jp in a computer network of RIKEN(The Institute of Physical and Chemical Research) in Japan.  I am writing now to notify my address and subscribe to the network for futher correspond. I am working
 on transformation and gene isolation of Arabidopsis thaliana.
I hope this mail reaches correctly to arabidopsis e-mail network.
If you come across this mail, could you kindly send me a "ECHO"?
Thank you.

                             Bong J. Kang
                             Lab. for Plant-Hormone Function
                             FRP, RIKEN, Saitama, Japan
                             Fax : 81-48-462-4691
                             e-mail : bjkang@rkna50.riken.go.jp
From: slblowe@uccs.edu
Newsgroups: bionet.genome.arabidopsis
Subject: POSTDOC POSITION
Date: 31 Aug 92 15:08:06 GMT
Distribution: bionet

DEAR NETWORKERS,
PLEASE BRING THE FOLLOWING AD TO THE ATTENTION OF SUITABLE CANDIDATES.SANDY
BERRY-LOWE.

ONE USDA-FUNDED POSTOCTORAL POSITION IS AVAILABLE IMMEDIATELY TO STUDY THE
BIOCHEMISTRY, MOLECULAR BIOLOGY, AND GENETICS OF THE BIOSYNTHESIS OF DELTA-
AMINOLEVULINIC ACID IN ARABIDOPSIS. ALA IS THE PRECURSOR OF HEME AND 
CHLOROPHYLL IN PLANTS. 3 ENZYMES ARE INVOLVED IN THE SYNTHESIS OF ALA.
GLUTAMYL-REDUCTASE AND GLUTAMATE SEMIALDEHYDE AMINOTRANSFERASE WILL BE
ISOLATED AND OVEREXPRESSED IN ECOLI (SEE PLANT PHYSIOL. 99, 1597; 1992).
AND CHARACTERIZED. PH.D IN BIOCHEMISTRY OR MOLECULAR BIOLOGY IS REQUIRED.
POSITION IS FOR 2 YEARS. PLEASE SEND A CV AND HAVE 3 LETTERS OF REFERENCE
SENT TO:
	SANDY BERRY-LOWE
	DEPARTMENT OF BIOLOGY
	UNIVERSITY OF COLORADO-COLORADO SPRINGS
	1420 AUSTIN BLUFFS PARKWAY
	COLORADO SPRINGS, CO 80907

	TELE: 719-593-3671
	FAX: 719-593-1239
From: AGOOD@VM.UCS.UALBERTA.CA
Newsgroups: bionet.genome.arabidopsis
Subject: Arabidopsis Network
Date: 26 Aug 92 23:02:54 GMT
Distribution: bionet

From: Allen Good                       Assistant Professor
                                       Department of Genetics
                                       University of Alberta

I have received this E-Mail address to be put on the Arabidopsis Network.
Could you please send me information on how to become a member of the network
and tologon, plus any information you would have on the Arabidopsis stock
centre. My E-Mail address is AGOOD@VM.UCS.UALBERTA.CA
                                       Regards,
                                       Allen Good
From: MORRISJ@WSUVM1.CSC.WSU.EDU
Newsgroups: bionet.genome.arabidopsis
Subject: Re: electroporation
Date: 30 Aug 92 04:52:20 GMT
Distribution: bionet

On 28 Aug 92 10:39:50 GMT Neil Butt said:
>Dear networkers,
>We are about to embark on some plant transformation experimentation, using
>electroporation. All the protocols we have seen use 0.4ml cuvettes for
>plant cells. Our group has used electroporation techniques on yeast using
>0.2ml cuvettes. This may seem a petty factor, but we have not performed
>these experiments before and do not want to take any chances, but is it O.K
>to use the smaller sized cuvettes. We intend to do these experiments in both
>Tobacco and Arabidopsis in case the plant species is a factor in the
>protocol.
>Hope someone can help
>Neil Butt
>Biological Sciences
>University of Sussex
>Falmer
>Brighton
>East Sussex, BN1 9QG.
>U.K
>E-mail     bafp3@uk.ac.sussex.central
>(from non-UK)   bafp3@central.sussex.ac.uk
>
Dear Neil,

If you chose to use 0.2 cm cuvettes for electroporation, I'm confident that it
would work, but I'd suggest reducing the voltage setting by half.  Reducing
the cuvette width increases the field strength (volts per cm), and I'd
anticipate, reduces the pulse time.  I've had success with tobacco and
arabidopsis protoplasting in a thiosulfate buffer (Zaghmout et al. 1990, In
Vitro Cell. Dev. Biol. 26:315-317) and electroporating in a 0.45 M sucrose
buffer.  Pulse length seems to be important, I adjust cuvette volumes to get
an approximately 50-70 msec decay time.  Hope this helps.
John Morris  [morrisj@wsuvm1.csc.wsu.edu]
From: dkw@PLANTPATH.WISC.EDU ("D. Kyle Willis")
Newsgroups: bionet.genome.arabidopsis
Subject: (none)
Date: 2 Sep 92 01:38:11 GMT
Distribution: bionet

Ph.D. AND POSTDOCTORAL STUDIES IN PLANT BIOLOGY

UNIVERSITY OF WISCONSIN

	A new training program for graduate students and postdoctoral 
fellows, funded by the DOE/NSF/USDA Collaborative Research in 
Plant Biology Program, is accepting applications.  The focus of 
this group is use of a model higher plant, Arabidopsis thaliana, 
for research in plant signal transduction. The research plan has 
flexibility to accommodate candidates with different backgrounds 
and interests.  The purpose is to provide broad, 
multi-disciplinary training in genetics/molecular biology, 
biochemistry, electrophysiology, and anatomy/immunocytochemistry.  
The 12 participating faculty include: Amasino, Barton, Becker, 
Bleecker, Evert, Fernandez, Keegstra, Kung, Masson, Sussman, 
Vierstra, and Willis.  Interested students/postdocs should apply 
by writing to:

		Professor Michael R. Sussman
		University of Wisconsin
		Plant Sciences Building (Moore Hall)
		1575 Linden Drive
		Madison, WI  53706-1590
		<SUSSMAN%PSL.DECNET@MACC.WISC.EDU>
From: dkw@PLANTPATH.WISC.EDU ("D. Kyle Willis")
Newsgroups: bionet.genome.arabidopsis
Subject: Ph.D. AND POSTDOCTORAL STUDIES IN PLANT BIOLOGY at UW-Madison
Date: 2 Sep 92 02:10:02 GMT
Distribution: bionet

Ph.D. AND POSTDOCTORAL STUDIES IN PLANT BIOLOGY

UNIVERSITY OF WISCONSIN

	A new training program for graduate students and postdoctoral 
fellows, funded by the DOE/NSF/USDA Collaborative Research in 
Plant Biology Program, is accepting applications.  The focus of 
this group is use of a model higher plant, Arabidopsis thaliana, 
for research in plant signal transduction. The research plan has 
flexibility to accommodate candidates with different backgrounds 
and interests.  The purpose is to provide broad, 
multi-disciplinary training in genetics/molecular biology, 
biochemistry, electrophysiology, and anatomy/immunocytochemistry.  
The 12 participating faculty include: Amasino, Barton, Becker, 
Bleecker, Evert, Fernandez, Keegstra, Kung, Masson, Sussman, 
Vierstra, and Willis.  Interested students/postdocs should apply 
by writing to:

		Professor Michael R. Sussman
		University of Wisconsin
		Plant Sciences Building (Moore Hall)
		1575 Linden Drive
		Madison, WI  53706-1590
		<SUSSMAN%PSL.DECNET@MACC.WISC.EDU>
From: nam@POSTECH.AC.KR (Hong-Gil Nam)
Newsgroups: bionet.genome.arabidopsis
Subject: (none)
Date: 27 Aug 92 10:26:56 GMT
Distribution: bionet


 I have not received the Arabidopsis news letter since January, 1992.
I am sending you my E-mail address. Would you please send me the newsletter through E-mail. It could be nice to receive newsletters including the past ones since January 1992.

Thank you.
              Hong-Gil Nam, ph.D
                 Aug.27.1992

E-mail :nam@vision.postech.ac.kr
Fax:0562-79-2199
Tel:0562-79-2111 

Dr.Hong-Gil Nam 
Dept.of lifescience
POSTECH
P.O.box 125, Pohang Kyungbuk
790-600
South Korea
From: BTNYLAC@MVS.UCC.OKSTATE.EDU
Newsgroups: bionet.genome.arabidopsis
Subject: RNA from siliques
Date: 28 Aug 92 01:28:00 GMT
Distribution: bionet

Brian,
I also got a rusty color contaminant in silique
RNA preps with the phenol/LiCl method.  I switched
to guanidinium thyocyanate/ CsCl and got clean RNA.
Some references for this method are:
Chirgwin et al Biochemistry 18:5294-99 (1979)
Kopczynski and Scandalios MGG 203:185-8 (1986)
and Current Protocols in Molecular Biology 4.2
Hope this helps.
P.S. The rusty contaminant hung in the wells of
agarose gels and the RNA still ran fine.
Linda Castle, Botany, Oklahoma State University
Xref: sparky bionet.biology.tropical:70 bionet.population-bio:114
From: una@phy.duke.edu (Una Smith)
Newsgroups: bionet.biology.tropical,bionet.population-bio
Subject: Monarch butterfly migrations
Summary: Participate in butterfly marking program
Date: 3 Sep 92 18:55:49 GMT
Followup-To: bionet.biology.tropical
Distribution: usa
Organization: Duke University Physics Dept.; Durham, N.C.


This announcement appeared on the CONSLINK list.  Please reply
to the original sender (name and address below), not me.  Thanks.

	Una

---------------------- start of announcement --------------------

Dr. Orley Taylor and I (Brad Williamson) are attempting to
coordinate a Monarch butterfly marking effort in the eastern U.S.
The eastern population ranges to the front range of the Rockies.
Last winter on the Monarch's wintering sites in Mexico some of the
roosts suffered heavier than normal losses.  Personal
communications with widespread individuals indicate that the
resulting summer populations of the Monarchs are quite low for
much of the eastern U.S. and Canada with particularly low numbers
noted in the N.E.  There is apparently no long-term threat to the
Monarch population but this low number represents a unique
opportunity for significant population studies.  The migration is
beginning throughout the northern parts of the Monarch's range and
we would like to mark butterflies over a wide geographical area.
We are looking for interested schools (secondary and
post-secondary), nature groups, and qualified individuals who want
to take part in this study.  We currently have enrolled approx. 40
teachers and their students from Ks. and a number of individuals
from Minn.  Realize that this is a non-funded project...it's
coming out of our own pockets...so we may have to be selective.
This is a great opportunity for secondary students to take part in
valid scientific research.  If you are interested in the project
please contact me ASAP.

Brad Williamson
Olathe East High School
14545 W. 127th Street
Olathe, KS 66062
Phone: (913) 780-7120
E-mail: bwilliam@ksuvm.ksu.edu or williamsonks@igc.apc.org
----------------------- end of announcemment -------------------------


-- 

Una Smith   una@phy.duke.edu    Department of Biology
                                Yale University
                                New Haven, CT  06511-7444
From: erik@csa.bu.edu (Erik McCormick)
Newsgroups: bionet.biology.computational
Subject: Urgent question about a Hitachi chip
Date: 23 Aug 92 17:37:31 GMT
Distribution: bionet
Organization: Computer Science Department, Boston University, Boston, MA, USA
Originator: biocomp@lerkim.umiacs.umd.edu


URGENT PLEASE!!!!!!!

If any one has any information on the malfuntion of:

Hitachi chip: 6063 date code 0a2q and 9b38


Apparently a batch produced in malyasia from about 1990 Jan 12-18
(batch size about 100,000?) have an abnormality in the optical dye.

In most cases the chps are fine, except in some situations when there
are malfunctions (duhh!!) after hibernation.  However, if the chips are 
asked to rehibernate, and then taken out, the chips works fine.  In 
standard testing the chips perform properly as well.  Any assistance or 
information that you might be able to offer is greatly appreciated.

Please mail any information to honjo1@husc.harvard.edu.  


-Yuki Honjo
From: robert.h.gross@dartmouth.edu (Robert H. Gross)
Newsgroups: bionet.biology.computational
Subject: Re: A Wish-List for Gene Identification Programs
Date: 29 Aug 92 19:45:00 GMT
Distribution: bionet
Organization: Dartmouth College, Hanover, NH
Originator: biocomp@lerkim.umiacs.umd.edu

Eric-

I'd be very interested in seeing a tabulation of your responses posted
to the net. I agree that there has been no consistent way to compare
different algorithms. Perhaps what is needed is a standard set of DNA
sequences to use for the analysis?

GOOD QUESTION!

Bob Gross
bob.gross@dartmouth.edu
From: pat@mito (Pat Gillevet)
Newsgroups: bionet.biology.computational
Subject: Director of Computation, Harvard Genome Lab
Date: 25 Aug 92 21:37:30 GMT
Distribution: bionet
Organization: Harvard Genome Laboratory
Originator: biocomp@lerkim.umiacs.umd.edu



                         DIRECTOR OF COMPUTATION
                        HARVARD GENOME LABORATORY
                           HARVARD UNIVERSITY



The Harvard Genome Lab is seeking  an  experienced  UNIX/Xwindows
programmer with experience in Computational Biology to direct the
analysis of genomic data from the Mycoplasma capricolum project.

The present computational environment at the Harvard  Genome  Lab
is based on the Genetic Data Environment (GDE), a X-Windows based
Graphic User Interface, which allows the seamless integration  of
a  core  multiple  sequence editor with pre-existing external se-
quence analysis programs and internally developed
 programs into a single prototyping environment.  Furthermore, we
have  incorporated  our internal database and automated data con-
trol systems into the Graphic User Interface to handle both  ran-
dom fluorescent sequencing data and genomic walking data from the
Mycoplasma project at the Harvard Genome Lab.  We are now in  the
process  of expanding the database and integrating it into a Pro-
log based system.

The successful candidate would oversee  a  system  administrator,
scientific  programmer  and  a software support technician.  Pro-
gramming duties would include the support of  automated  sequence
analysis,  Integrated Genome databases, and X-Windows based tools
for controlling the production process.

Please submit CV and brief statement of interests via email to

Patrick M. Gillevet
Director  Harvard  Genome  Lab
Harvard University
16 Divinity Ave.  Cambridge, MA  02138

Phone: 617-496-9111
Fax  : 617-495-9300
email:  pat@nucleus.harvard.edu