From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: N.tabacum gene for pectate lyase
Date: 29 Dec 92 20:58:58 GMT
Distribution: bionet
Checksum: 48138 5

LOCUS       TOBPECL1     1713 bp ds-DNA             PLN       11-DEC-1992
DEFINITION  N.tabacum gene for pectate lyase
ACCESSION   X67158
KEYWORDS    pectatelyase.
SOURCE      Nicotiana tabacum DNA.
  ORGANISM  Nicotiana tabacum
            Eukaryota; Plantae; Embryobionta; Magnoliophyta; Magnoliopsida;
            Asteridae; Solanales; Solanaceae.
REFERENCE   1  (bases 1 to 1713)
  AUTHORS   Rogers,H.J., Harvey,A. and Lonsdale,D.M.
  TITLE     Isolation and characterization of a tobacco gene with homology to
            pectate lyase which is specifically expressed during
            microsporogenesis
  JOURNAL   Plant Mol. Biol. (1992) In press
  STANDARD  full automatic
COMMENT     *source: strain=Samsun; *source: stage=mature pollen; *source:
            clone_library=lambda EMBL3; *source: clone=G10; *source:
            is_germline=Y;
               From EMBL    entry NTPECL1;  dated 08-JUL-1992.
FEATURES             Location/Qualifiers
     mRNA            join(108..901,1000..1300,1398..1713)
     mat_peptide     join(400..901,1000..1300,1398..1710)
                     /product="pectate lyase"
                     /codon_start=1
     exon            108..901
                     /number=1
     prim_transcript 108..1713
     transit_peptide 325..399
     intron          902..999
                     /number=1
     exon            1000..1300
                     /number=2
     intron          1301..1397
                     /number=2
     exon            1398..1713
                     /number=3
     CDS             join(325..901,1000..1300,1398..1713)
                     /product="pectate lyase"
                     /codon_start=1
                     /translation="MDVYRIRISVFFLLVLLTFAALTTATNIPRRQLSNKKYKGPCRA
                     ENAIDKCWRCDPNWAENRQKMADCALGFGSNAIGGKLGRIYVVTDNSDDDVVDPKPGT
                     LRYGVIQKEPLWIIFGKNMKIKLSRELIVTSNKTIDGRGFNVHIQNGAGIKIQSASNI
                     IISNLRIHNIVPTPGGLLRESEDHVGLRGSDEGDGISIFSSHDIWIDHISMSRATDGL
                     IDAVAASTNITISNCHFTDHEKVMLFGANDHYVLDKDMKITLAYNHFGKRLDQRMPRC
                     RFGFFHLVNNDYTHWERYAIGGSSGATIISQGNRFIAEDELLVKEVTYREKLTASVAE
                     WMKWTWISDGDDMENGATFTPSGDQNLLDKIDHLNLIKPEPSSKVGILTKFSGALSCV
                     KGRPC"
BASE COUNT      546 a    338 c    353 g    476 t
ORIGIN      
        1 ggccattaac gagtgacaca ccatagttat cgctccttat ttcaactaat ggacccaaat
       61 gctctgcgct atcactatat aaggaacaat atatgctgtg aatgaaaata agcaaaaaag
      121 agaaacaaat aatgtggctt gtttaggaat aatcctgatt ttagaatttt ctcacaaact
      181 tttctcaggg ctggtttaat aagatcacgt tggtgaaaag gattgacctt gcaccattta
      241 acatcaacca tcaatcagcc cagcaaacaa attacaacca cctgactcta actctctaaa
      301 catttcaaga ttaccaatag ataaatggac gtctacagaa taagaattag tgtatttttt
      361 ctccttgtgt tactcacatt tgctgcatta acgacagcaa caaacatccc aagaaggcaa
      421 ctcagtaaca agaaatacaa aggcccatgc cgggccgaaa atgcaatcga caaatgctgg
      481 cgatgcgacc caaattgggc cgagaatcgc cagaaaatgg ccgattgcgc attgggcttt
      541 ggcagcaatg cgatcggagg aaagttgggc cgaatttacg tcgtcacgga caattctgac
      601 gacgatgttg tcgatcctaa gcccggaact ctccgatatg gcgttatcca aaaggagcca
      661 ttgtggatca tatttggcaa aaacatgaaa attaagctta gtagggaact tatcgtgact
      721 agcaacaaaa ctatcgacgg ccgaggattt aatgtccata tacaaaatgg agctggcatt
      781 aagatacaat ctgcatccaa tattattatt tctaacctcc gtattcacaa tattgtaccc
      841 accccaggtg gcctgctcag ggaatctgag gaccacgttg gtctaagggg tagcgatgaa
      901 ggtattgttg aattgtgtag ctatctcatc ttaagaattt aagctattag aaagaatgca
      961 ctttttaatt aattattgaa ttatattacg tctcaacagg tgatggcatc agcatcttca
     1021 gttcgcatga tatatggatc gatcacattt ccatgtctcg tgcaaccgat ggtcttatcg
     1081 atgctgttgc tgcttccact aacattacca tatccaattg ccacttcact gaccacgaaa
     1141 aagtaatgtt gttcggcgcc aacgatcatt acgtgcttga caaggacatg aaaattacat
     1201 tagcgtacaa ccactttgga aagaggttgg atcaaaggat gccaagatgc aggtttggat
     1261 ttttccatct ggtgaacaat gactacactc attgggagag gtatacacac tattgaattg
     1321 tctagccgtc gcatttaaat aagctagaaa atacgattcg attaaattac gtaattatac
     1381 tatgtctgta ttgataggta cgctattggc ggaagcagtg gagccaccat tattagccag
     1441 ggtaatcgat ttattgctga ggatgaattg ttggtgaaag aagtaacata tagagaaaaa
     1501 cttacggcga gtgtggcgga atggatgaaa tggacttgga tatcagatgg tgatgatatg
     1561 gaaaatggtg ctacatttac accatctggt gaccagaatt tactcgataa aattgaccat
     1621 ctcaatctga ttaaaccaga accttcttct aaagttggaa ttcttactaa gttttctggt
     1681 gctctgtctt gcgtcaaagg acgtccatgc taa
//