HDTenEightyP/NetNews huggingface dataset:
From: James Petts <pettsj@visigoth.demon.co.uk>
Subject: Re: Zn binding to polyhistidine
Organization: No Affiliation
Date: Fri, 28 Aug 1992 06:46:54 GMT
X-Useragent: Nuntius v1.1



In article <01GO32J3NJNY9357M2@minna.iit.edu> , CMSMUELLER@KARL.IIT.EDU
writes:
>Does anyone know the relationship between the number of 
>histidine residues and the binding affinity to Zn++?  

Therw was an article in "Protein Engineering" somewhen in 1991 which look 
at binding of metal ions to his and asp/glu residues in proteins. 
Also, the residues which bind a Zn ion seem to be dependent on the nature 
of the substrate of the protein. e.g., astacin, stromelysin, collagenase, 
thermolysin, etc, etc, which have a neutral substrate, bind the Zn with 
two his and one asp/glu residue, whereas e.g. carbonic anhydrase has 3 
his residues. That's about the limit of my knowledge. Hope it helps.


James Petts
********************************************************************
* "We use the classical theory on Mondays, Wednesdays and Fridays, *   
*  and the quantum theory on Tuesdays, Thursdays and Saturdays."   *
************************************** Wm. Bragg *******************
From: POSTMAST@GUNBRF.BITNET
Newsgroups: bionet.molbio.proteins
Subject: Announcement of PIR Network Request Server Downtime
Date: 3 Sep 92 17:47:30 GMT
Distribution: bionet


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From: CMSMUELLER@KARL.IIT.EDU
Newsgroups: bionet.molbio.proteins
Subject: Zn binding to polyhistidine
Date: 27 Aug 92 23:55:57 GMT
Distribution: bionet

Does anyone know the relationship between the number of 
histidine residues and the binding affinity to Zn++?  
We are working with an enzyme with 3 identical subunits.  
We would like to make hybrid enzymes containing 1, 2, and 
3 mutant subunits, purify them, and analyze the kinetics.  
The present scheme is to add 3 His residues on the end of 
the wild type subunit by genetic engineering, expressing 
this in the presence of the gene coding for the mutant 
subunit, and purifing the hybrid molecules by Zn chelate 
affinity chromatography.  For this to work, all three of 
the hybrid molecules must differ significantly in their 
ability to bind to Zn++.  

Any suggestions?

Thanks

David Mueller
Department of Biological Chemistry
The Chicago Medical School
North Chicago IL
Newsgroups: bionet.molbio.proteins
From: James Petts <pettsj@visigoth.demon.co.uk>
Subject: Re: Zn binding to polyhistidine
Organization: No Affiliation
Date: Fri, 28 Aug 1992 06:49:50 GMT
X-Useragent: Nuntius v1.1



In article <01GO32J3NJNY9357M2@minna.iit.edu> , CMSMUELLER@KARL.IIT.EDU
writes:
>Does anyone know the relationship between the number of 
>histidine residues and the binding affinity to Zn++? 

Furthermore, in all the cases I mentioned in the previous posting, two of 
the histidine residues are on a helix, separated by three other residues 
(i.e. they are residues n and n+4, i.e. on the same face of the helix, 
which repeats with period 4 of course.)

Fee free to e-mail me if you want me to look at the structures in more 
detail.
 
James Petts
********************************************************************
* "We use the classical theory on Mondays, Wednesdays and Fridays, *   
*  and the quantum theory on Tuesdays, Thursdays and Saturdays."   *
************************************** Wm. Bragg *******************
Newsgroups: bionet.molbio.proteins
From: kgg6519@uxa.cso.uiuc.edu (Kamalakar  Gulukota)
Subject: Aggregation of proteins on refolding
Organization: University of Illinois at Urbana
Date: Mon, 31 Aug 1992 14:42:36 GMT

	Here is a question on Protein refolding experiments.  Can someone
please answer it.

	Many proteins on refolding give only partial activity because the
rest of the protein has aggregated.  It is supposed that it is really
some partially folded intermediates on the folding pathway that form
interactions with other molecules rather than have intramolecular
interactions to form the native structure.  How much of the protein
(what %) aggregates depends upon the nature of the protein as well as,
in some cases, on the method of refolding (or, perhaps more to the point,
the method in which the protein was denatured in the first place).  Now,
the question is this:  Does it make sense to talk about a single number
as the percentage of the protein that aggregates?  i.e. is there a limit
that aggregation reaches?  If I found 30% aggregation at 2 hours, would
I find 35% at 3 hours and so on?  If I waited long enough, would aggregation
in any protein refolding process reach 100%?

	Undoubtedly, the RATE of aggregation decreases with time.  But does
it ever get close enough to zero that we can say that the aggregation
at that time is the limit and that at time = infinity, the % aggregation
would still be about the same?

	Thanks very much in advance

Gulu

Newsgroups: bionet.molbio.ageing
From: dman@netcom.com (Dallman Ross)
Subject: Re: Thanks for making the newsgroop alive
Date: Thu, 27 Aug 92 04:46:56 GMT
Organization: Netcom - Online Communication Services  (408 241-9760 guest) 
X-Newsreader: Tin 1.1 PL4

Oh, no problem!  :-)

I don't know why you couldn't reach me.  Both my active addresses are in my
.signature file, below.  (The second gets forwarded to the first auto-
matically.)

--
 __D_a l_l m a_n  _ R o_s s _ |dman@netcom.com  /or/  |"You sound like a man|
 l  \\ l\\   /l  /\\  l\\   l |dross@well.sf.ca.us    |with a rubber nose." |
 l   >)l \\ /ll /  \\ l \\  l |vox/fax: 1.510.645.1883| -- One-Lung Bill    |
 l  // l  \X ll/--- \\l  \\ l |350 Perkins St., #108  |     Remmer (deceased)
_l//______________________\\l_|Oakland,_CA__94610-3422|_____________________|_
From: macko@pilot.njin.net (Peter C. Macko)
Newsgroups: bionet.molbio.ageing
Subject: Re: Why does calorie restriction reduce the rate of aging?
Date: 26 Aug 92 14:50:50 GMT
Organization: Rutgers Univ., New Brunswick, N.J.


 bradbury@sftwks.UUCP (Robert Bradbury) writes:

>When one discusses aging one has to start with some facts (animals fed less
>live longer than those fed more), propose a theory which is consistent

  Have there been any studies that have varied the sources of the
calories fed to CR animals?  

>with natural selection (one would want to survive times when food is
>scarce and most animals in the wild do not live to reach "old age") and
>then look for biochemical explanations which connect the two.  I would
>do the same thing *if* caloric excess led to longer lifespan but it
>doesn't.  Animals who have lots of food on an ongoing basis would evolve
>not a longer lifespan but faster growth and increased reproductive capacity
>because that is what would increase the genes of those individuals in
>the gene pool.  In fact natural selection works against this because
>species which over-reproduce exhaust their food resources leading to
>population crashes and possible extinction.

  Is there any measurable difference in the metabolic rates of CR animals
and animals on a "normal diet" throughout different points in their 
lifespans before the normal diet animals reach "old age"?  Are youthful
animals on a normal diet more energetic  then those on a restricted diet?
At what point in life, if any, does this get reversed?  Should the CR diet
start later in the animal's life, does it have the same, somewhat reduced,
or no impact at all?  

  Please excuse my questions if they sound somewhat rudimentary.  This is 
not my area of expertise, but I do find this topic fascinating nonetheless.
So if there are any good scientific references on this subject and the
studies done to date please recommend them.


					    -PCM
  
-- 

  Peter C. Macko                      Internet: macko@pilot.njin.net
  LCSR/Network Research                         macko@cs.rutgers.edu
  Telecommunications/Network Services     UUCP: {backbone}!rutgers!cs!macko
  Rutgers Computing Services,  Rutgers University (Piscataway, NJ)
From: rbradbur@hardy.u.washington.edu (Robert Bradbury)
Subject: Re: BOOK: Aging, Sex and DNA Repair (Bernstein and Bernstein)
Summary: A good book with some problems
Organization: University of Washington, Seattle
Date: Thu, 27 Aug 1992 05:25:56 GMT

In article <1992Aug25.220510.16933@leland.Stanford.EDU>
  cpatil@leland.Stanford.EDU (Christopher Kashina Patil) writes:
> Recomends the book: Aging, Sex, and DNA Repair

This book is a good overview of our current understanding of aging.
It received some discussion a couple of months ago in this group.
Of particular interest (at least to me) is the fact they argue that
each cell in our body has 70,000+ "hits" to DNA bases per day.
I've reviewed the sources for these claims and the evidence seems
fairly solid.  The unknown factor is to what degree repair of this
damage is insufficient or erroneous so as to result in cells less able
to respond to stress or containing mutated genes.

>	I am extremely poorly versed in the controversies surrounding the 
>various theories on the evolution of sex, so I will refrain from summarizing
>the authors' arguments  on this issue. Suffice it to say that I found the  
>discussion provocative.
>
There has been a recent review of this book by AS Kondrashov (Trends in
Genetics (1992) 8(4):149-150).  He argues that the authors have ignored
some of the current literature in their arguments regarding the evolution
of sex.  A recent article by Kondrashov which people may want to read as
well is in Nature (1988) 336(6198) 435-440.

Robert Bradbury		uunet!sftwks!bradbury or rbradbur@u.washington.edu
From: Mark_Guyer@OCCSHOST.NLM.NIH.GOV
Newsgroups: bionet.molbio.genome-program
Subject: (none)
Date: 4 Sep 92 01:01:00 GMT
Distribution: bionet


          For the most up-to-date information on Chr 17 hybrids, I
          suggest that you contact Dr. David Ledbetter at the Baylor
          College of Medicine [(713 798-6511)].  There is also a
          Chromosome 17 bulleting board that is run out of Baylor by
          Dr. Bob Cottingham.  Ledbetter can tell you how to get in
          touch with him.

          The other resource that you might look into is a Chr 17
          radiation hybrid panel developed by Dr. Ray White at the
          University of Utah [(801) 581-4330)].
From: Mark_Guyer@OCCSHOST.NLM.NIH.GOV
Newsgroups: bionet.molbio.genome-program
Subject: Projects
Date: 4 Sep 92 00:56:00 GMT
Distribution: bionet


          We don't have a list of NCHGR-supported projects in
          e-mailable form.  If you let me know your address, I can
          have a report mailed to you.

Newsgroups: bionet.molbio.genome-program
From: michael@uwovax.uwo.ca
Subject: Projects
Organization: University of Western Ont, London
Date: Tue, 1 Sep 1992 13:48:45 GMT

Would anyone be able to provide a listing summary of projects currently
funded by the Genome Project?  Email directly to me would be greatly
appreciated.
Thanks,
Michael Clarke
michael@uwovax.uwo.ca
From: weigeld@STARBASE1.CALTECH.EDU ("WeigelD")
Newsgroups: bionet.jobs
Subject: FW: SJapan: bio ctrl. research
Date: 31 Aug 92 04:06:13 GMT
Distribution: bionet


L Godfrey writes:

>Regarding your choice of Germany or Japan, Germany is, in my opinion, the
>most appalling country to work in.  I worked there for two years, and the
>people are utterly humorless, racist, arrogant, and never admit to being
>wrong.  Furthermore, Asians of all kinds, particularly Orientals (my wife
>is one) are frequently threatened and often attacked by local neo-nazis.
>Finally it is expensive, the food is revolting, the rents are high, cars
>are outrageously expensive (particularly used cars), the bureacracy is
>impenetrable and arrogant (especially the Immigration people) and the country
>offers very little by way of amusement or entertainment.

What do you call somebody who condemns a people wholesale as racist?

A racist.

By the way, for an impenetrable Immigration bureaucracy, try the INS.

Detlef Weigel
Caltech 



Newsgroups: bionet.jobs
Tue, 1 Sep 1992 08: 46:28 +0100
               Tue, 1 Sep 1992 07: 59:08 +0100
               Tue, 1 Sep 1992 10: 41:31 +0100
From: suter@de.dbp.MPG.MPIZ-KOELN.VAX
Newsgroups: bionet.jobs
Subject: have this stopped simply
Date: 1 Sep 92 09:41:31 GMT
Distribution: bionet
Content-Identifier: 12
Original-To: biojobs@uk.ac.daresbury
Conversion: Prohibited

Subj:	Re: FW: SJapan: bio ctrl. research
L.godfrey wrote:

<In article <9208311903.AA19984@genbank.bio.net>, weigeld@STARBASE1.CALTECH.EDU
< ("WeigelD") writes...
<>
<>L Godfrey writes:
<>
<>>Regarding your choice of Germany or Japan, Germany is, in my opinion, the
<>>most appalling country to work in.  I worked there for two years, and the
<>>people are utterly humorless, racist, arrogant, and never admit to being
<>>wrong.  Furthermore, Asians of all kinds, particularly Orientals (my wife
<>>is one) are frequently threatened and often attacked by local neo-nazis.
<>>Finally it is expensive, the food is revolting, the rents are high, cars
<>>are outrageously expensive (particularly used cars), the bureacracy is
<>>impenetrable and arrogant (especially the Immigration people) and the country
<>>offers very little by way of amusement or entertainment.
<>
<>What do you call somebody who condemns a people wholesale as racist?
<>
<>A racist.
<>
<>By the way, for an impenetrable Immigration bureaucracy, try the INS.
<>
<>Detlef Weigel
<>Caltech
<>
<
<The Germans are a nation, not a race.  Criticism of national characteristics
<is legitimate, particularly if those characteristics are so prevalent and
<objectionable as they are in the Germans.
<
<I agree that the INS is awful (I am not American), but the US has compensations
<whereas Germany has none.
<
<LG

i am not german, i am dutch. as some of you may remember, the dutch have had 
some (minor) problems with the german nation in the past (before i was born, 
actually).
But the reaction of L. Godfrey (probably based on personal experience ?)
is extremely reactionary, AND DOES NOT BELONG ON THE NET !

(the only hope i have is that D. Kristofferson puts an end to it now, so that
it won't linger on for the next few weeks)

clemens
----------------------------------> cut here <----------------------------------
Clemens Suter-Crazzolara, PhD
Max-Planck-Institut fuer Zuechtungsforschung
Abteilung Genetische Grundlagen der Zuechtungsforschung
Carl-von-Linne Weg 10
5000 Koeln 30
Tel. xx49-221-5062.221           fax. xx49-221-5062.21
suter@vax.mpiz-koeln.mpg.dbp.de
-------------------------------> end of message <-------------------------------
From: cjs@mink.mt.att.com (Charanjit Singh)
Newsgroups: bionet.jobs
Subject: UNIX/C/DATABASE Professional seeks job in Canada, Europe or Australia
Date: 2 Sep 92 20:45:09 GMT
Distribution: bionet

Keywords: Unix/C/Oracle/Informix/Application or Systems programming

Unix/C/Oracle/Informix/Application or Systems programming professional seeks
contract or permanent position in the Canada, Europe or Australia.

        o Have MS in Computer Technology and Seven years' experience
          in the data processing industry.

        o Skilled in Application/Systems Programming, Database
          Administration, Design of Database Managers, Performance Tuning,
          Systems Administration, Unix Kernel and Device Drivers.

        o Have ability to communicate effectively.

        o Very self-motivated and strongly confident of taking challenges.

Please send email to cjs@mink.att.com for resume or more details.

+-+-+-+-+-+-+-+-+-+-+-+-+-+-+-+-+-+-+-+-+-+-+-+-+-+-+-+-+-+-+-+-+-+-+-+-+-+-+
|    Charanjit Singh, AT&T Bell Laboratories, Middletown, NJ 07748 (USA)    |
| Phone: (908) 957-3309     Fax: (908) 957-4428     Email: cjs@mink.att.com |
+-+-+-+-+-+-+-+-+-+-+-+-+-+-+-+-+-+-+-+-+-+-+-+-+-+-+-+-+-+-+-+-+-+-+-+-+-+-+
Newsgroups: bionet.jobs
From: samodena@csemail.cropsci.ncsu.edu (S. A. Modena)
Subject: Re: SJapan: bio ctrl. research
Organization: Crop Science Dept., NCSU, Raleigh, NC 27695-7620
Date: Tue, 1 Sep 1992 05:31:07 GMT

In article <1327000002@igc.apc.org> anlyyao@igc.apc.org (An-Ly Yao) writes:
>
>keywords: entomology, bio control, hymenoptera, mass breeding, QC, Shanghai,
>          Japan, scientific illustration (GNSI)
>-------------------------------------------------------------------------------
> 
> 
>In middle or at the end of 1993 I might move from Korea to Japan to continue
>my work there for a German company. The decision wether or not to make that
>move depends on what scientific environment my wife (US educated entomologist)
>could find in Japan. (Alternative would be Germany, Munich).
> 

Well, we've had a fairly loathsome anti-German tirade, so let's balance it
out a bit.

In a very recent Nature (no earlier than 1 July 92) there appeared a Letter
to the Editor on the subject of Official Treatment (or mistreatment) of
foreign nationals, especially professional scientists, with respect to
Work Permits and Permanant Residence in Japan.  I believe that the author
of that letter would not mind being contacted and he may offer further
references on your specific needs in making a choice between DeutcheLand
Nippon. Sorry, I don't have the specific issue of Nature at hand: it
definitely was a Letter to the Editor and not an article or news item.

On the German question, during some recent pre-dinner chit chat,
I had it pointed out to me recently by a Brit,
a Pole and a Hollander that Germany, unlike the U.S., turns no one
away who comes to that country in need.

Steve
---
+------------------------------------------------------------------+
|     In person:  Steve Modena     AB4EL                           |
|     On phone:   (919) 515-5328                                   |
|     At e-mail:  nmodena@unity.ncsu.edu                           | 
|                 samodena@csemail.cropsci.ncsu.edu                |
|                 [ either email address is read each day ]        |
|     By snail:   Crop Sci Dept, Box 7620, NCSU, Raleigh, NC 27695 |
+------------------------------------------------------------------+
         Lighten UP!  It's just a computer doing that to you.
OOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOO
From: kaiser@soma.umdnj.edu (Brian Kaiser)
Newsgroups: bionet.jobs
Subject: Aging Research Assistant
Summary: Research position available at the coriell institute, aging research
Keywords: Aging
Date: 2 Sep 92 17:39:30 GMT
Distribution: bionet

position available for research assistant in the coriell cell repositories.
to establish and maintain differentiated cell cultures from a variety of human
and animal tissue such as epithelial, endothelial, smooth muscle, keratinocyte
etc. cell cultures.  perform cell culture characterizations such as in vitro
life span and differentiated cell culture markers.  Contact the Coriell         Institute for Medical Research, 401 Haddon Avenue, Camden, New Jersey  USA 08103(609) 966-7377.  EOE/AA 
Newsgroups: bionet.jobs,ne.jobs,misc.jobs.offered
From: wrindone@world.std.com (Wayne P Rindone)
Subject: FlyBase Database Position
Organization: The World Public Access UNIX, Brookline, MA
Date: Tue, 25 Aug 1992 20:40:27 GMT

  Systems Analyst / Database Programmer
        for FlyBase Project at Harvard University, Cambridge

Job description:  Assist in designing and implementing a major scientific
relational database that will contain genetic and molecular data about
the fruit fly, Drosophila, a model organism for the Human Genome
Initiative.  The successful candidate will be able to demonstrate
experience and skills in relational database design and the SQL language,
Unix/C programming, and graphical user interface design.  Experience with
server/client distributed applications would be helpful.  Sybase
experience and interest in genetic or biological databases desirable but
not required.

Preferred background:  B.S. in computer sciences.  At least two years of
relational database experience.  

To apply:  Please send a resume, including the names of three references
together with a source code sample to:

FlyBase
c/o Wayne P. Rindone
Dept. of Cellular and Developmental Biology
Harvard University
16 Divinity Avenue
Cambridge, MA 02138

or by e-mail to:

flybase@nucleus.harvard.edu
Newsgroups: bionet.jobs
From: samodena@csemail.cropsci.ncsu.edu (S. A. Modena)
Newsgroups: bionet.jobs
Subject: Re: SJapan: bio ctrl. research
Keywords: trash
Date: 1 Sep 92 06:06:54 GMT
Organization: Crop Science Dept., NCSU, Raleigh, NC 27695-7620

In article <31AUG199212465469@sscvx1.ssc.gov> godfrey@sscvx1.ssc.gov (LAURENCE GODFREY) writes:
>
>Regarding your choice of Germany or Japan, Germany is, in my opinion, the
>most appalling country to work in.  I worked there for two years, and the
                                     ^^^^^^^^^^^^^^^^^^^^^^^^^^^^
>people are utterly humorless, racist, arrogant, and never admit to being
>wrong.  Furthermore, Asians of all kinds, particularly Orientals (my wife
                                                        ^^^^^^^^^^^^^^^^^^
>is one) are frequently threatened and often attacked by local neo-nazis.
^^^^^^

Well, he doesn't say it directly: was his wife threatened?  And you
say you stayed there TWO YEARS, subjecting your wife to these humiliations?
What won't people do to advance their careers?

>Finally it is expensive, the food is revolting, the rents are high, cars
>are outrageously expensive (particularly used cars), the bureacracy is
>impenetrable and arrogant (especially the Immigration people) and the country
>offers very little by way of amusement or entertainment.
^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^

Notice in the description of Japan (next) how Godfrey has us travelling all
over Asia (even several thousands of miles to Hindustan.....and unlike
DeutchLand, there is no AutoBahn to zip right into adjoining "civilized"
countries.

>
>Japan, on the other hand, is reasonably civilised and hospitable, not that
>expensive anymore, food is good, people are okay, close to interesting
>places (Thailand, Philippines, Malaysia, Indonesia, Cambodia, Laos, Burma
                                                                    ^^^^^^^^
>(bit awkward at the moment), Hong Kong, Singapore (if you like shopping malls)
  ^^^^^^^^^^^^^^^^^^^^^^^^^^
  Burma has been "awkward" for a very long time.  But if one looks at the
tourist lism, in the last 30 years some other of those countries have had
their share of "awkward" mass bloodshed (not counting anything the US has
had a hand in).

>and Indian subcontinent).
>
>Laurence Godfrey

Let's take this to one of the ALT.POLITICS.* groups where we can turn the
decibels up!

Steve
---
+------------------------------------------------------------------+
|     In person:  Steve Modena     AB4EL                           |
|     On phone:   (919) 515-5328                                   |
|     At e-mail:  nmodena@unity.ncsu.edu                           | 
|                 samodena@csemail.cropsci.ncsu.edu                |
|                 [ either email address is read each day ]        |
|     By snail:   Crop Sci Dept, Box 7620, NCSU, Raleigh, NC 27695 |
+------------------------------------------------------------------+
         Lighten UP!  It's just a computer doing that to you.
OOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOO