From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: E.coli CFA/I fimbrial operon cfa a, cfa b, cfa c, cfa e, cfa d'
Date: 15 Sep 92 10:02:52 GMT
Distribution: bionet
Checksum: 50643    16

LOCUS       ECOCFAIA     7175 bp ds-DNA             BCT       15-SEP-1992
DEFINITION  E.coli CFA/I fimbrial operon cfa a, cfa b, cfa c, cfa e, cfa d'
            genes, complete cds.
ACCESSION   M55661
KEYWORDS    colonisation factor antigen I; regulatory protein.
SOURCE      Escherichia coli (enterotoxigenic) DNA.
  ORGANISM  Escherichia coli
            Prokaryotae; Gram-negative facultatively anaerobic rods;
            Enterobacteriaceae.
REFERENCE   1  (bases 1 to 7175)
  AUTHORS   Hamers,A.M., Pel,H.J., Willshaw,G.A., Kusters,J.G.,
            van der Zeijst,B.A. and Gaastra,W.
  TITLE     The nucleotide sequence of the first two genes of the CFA/I
            fimbrial operon of human enterotoxigenic Escherichia coli
  JOURNAL   Microb. Pathog. 6, 297-309 (1989)
  STANDARD  full staff_entry
REFERENCE   2  (bases 1 to 7175)
  AUTHORS   Gaastra,W., Jordi,B.J., Mul,E.M., Hamers,A.M., McConnell,M.M.,
            Willshaw,G.A., Smith,H.R. and van der Zeijst,B.A.
  TITLE     A silent regulatory gene cfaD' on region 1 of the CFA/I plasmid NTP
            113 of enterotoxigenic Escherichia coli
  JOURNAL   Unpublished (1990)
  STANDARD  full staff_entry
REFERENCE   3  (sites)
  AUTHORS   Jordi,B.J., Willshaw,G.A., Van der Zeijst,B.A. and Gaastra,W.
  TITLE     The complete nucleotide sequence of region 1 of the CFA/I fimbrial
            operon of human enterotoxigenic Escherichia coli
  JOURNAL   DNA Sequence 2, 257-263 (1992)
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     sig_peptide     848..904
                     /gene="cfa a"
                     /codon_start=1
     CDS             848..1468
                     /product="colonisation factor antigen a"
                     /gene="cfa a"
                     /codon_start=1
                     /translation="MHKLFYLLSLLMAPFVANANFMIYPISKDLKNGNSELVRVYSKS
                     KEIQYIKIYTKKIINPGTTEEYKVDIPNWDGGLVVTPQKVILPAGASKSIRLTQFKIP
                     KKEEVYRVYFEAVKPDSKENVIDNKKLTTELSVNIIYAALIRSLPSEQNISLNISRNA
                     KKNIIIYNNGNVRAGVKDIYFCKSSNIDDNCVKKAYNKNIYPEKVI"
     mat_peptide     905..1465
                     /product="colonisation factor antigen a"
                     /gene="cfa a"
                     /codon_start=1
     CDS             1594..2106
                     /product="colonisation factor antigen b"
                     /gene="cfa b"
                     /codon_start=1
                     /translation="MKFKKTIGAMALTTMFVAVSASAVEKNITVTASVDPAIDLLQAD
                     GNALPSAVKLAYSPASKTFESYRVMTQVHTNDATKKVIVKLADTPQLTDVLNSTVQMP
                     ISVSWGGQVLSTTAKEFEAAALGYSASGVNGVSSSQELVISAAPKTAGTAPTAGNYSG
                     VVSLVMTLGS"
     sig_peptide     1594..1662
                     /gene="cfa b"
                     /codon_start=1
     mat_peptide     1663..2103
                     /product="colonisation factor antigen b"
                     /gene="cfa b"
                     /codon_start=1
     CDS             2171..4780
                     /product="colonisation factor antigen c"
                     /gene="cfa c"
                     /codon_start=1
                     /translation="MKHKKKNRLVVAISVALIPYIGVTGDIPDSFRDLWGEQDEFYEV
                     KLYGQTLGIHRIKTTPTHIKFYSPESILDKINLKKEKEKELSVFFTNSFSRNGNMSCQ
                     GNTTIQYNCNYIKTKSVDVIVDDVDNVVNLFIGNEFLDSEAHNDEYHQLSRNVKKAFI
                     QSQTINLSDSGKYKRLSISGNSALGITDTSYAVLNWWMNYNKSNGYSNNEKTINSLYF
                     RHDLDKRYYYQFGRMDRTDLSQSISGSFNFNLLPLPDIDGIRTGTTQSYIKNTDKFIA
                     SPVTVMLTNFSRVEAFRNDQLLGVWYLDSGVNELDTARLPYGSYDLKLKIFENTQLVR
                     EEIIPFNKGRSSIGDMQWDIFVQGGNIVNDNDRYIEKQNNHKSSINTGLRLPITKNIS
                     VQQGVSVIDNKSYYEGSLKWNSGILSGSLNSEFSFLWGDNAKGNYQSISYTDGFSLSF
                     YHNDKRVDNCGRNYNAGWSGCYESYSASLSIPLLGWTSTLGYSDTYSESVYKSHILSE
                     YGFYNQNIYKGRTQRWQLTSSTSLKWMDYNFMPAIGIYNSEQRQLTDKGGYISVTITR
                     ASRENSLNTGYSYNYSRGNYSSNELFVDGYMTSTNNGDYHEAGMRFNKNRHNAEGRLS
                     GRINNRFGDLNGSFSMNKNRNTNSTNHSLTGGYNSSFALTSDGFYWGGSTAGLTKLAG
                     GIIKVKSNDTKKNLVKVTGTLYGDYSLGSNDNAFIPVPALTPASLIIEDNNYGDNNIS
                     ILAPTNNDMFMLPGNVYPVEIETKVSVSYIGRGFDPNGTPLSGAHVLNEPHVILDEDG
                     GFSFEYTGNEKTLFLLKGRTIYTCQLGKNKVHKGIVFVGDVICDINSTSSLPDEFVKN
                     PRVQDLLAKNDKG"
     sig_peptide     2171..2236
                     /gene="cfa c"
                     /codon_start=1
     mat_peptide     2237..4777
                     /product="colonisation factor antigen c"
                     /gene="cfa c"
                     /codon_start=1
     CDS             4784..5866
                     /note="unpublished sequence"
                     /product="colonisation factor antigen e"
                     /gene="cfa e"
                     /codon_start=1
                     /translation="MNKILFIFTLFFSSGFFTFAVSADKNPGSENMTNTIGPHDRGGS
                     SPIYNILNSYLTAYNGSHHLYDRMSFLCLSSQNTLNGACPSSDAPGTATIDGETNITL
                     QFTEKRSLIKRELQIKGYKQFLFKNANCPSKLALNSSHFQCNREQASGATLSLYIPAG
                     ELNKLPFGGVWNAVLKLNVKRRYDTTYGTYTINITVNLTDKGNIQIWLPQFKSNARVD
                     LNLRPTGGGTYIGRNSVDMCFYDGYSTNSSSLEIRFQDDNSKSDGKFYLKKINDDSKE
                     LVYTLSLLLAGKNLTPTNGQALNINTASLETNWNRITAVTMPEISVPVLCWPGRLQLD
                     AKVKNPEAGQYMGNIKITFTPSSQTL"
     sig_peptide     4784..4843
                     /gene="cfa e"
                     /codon_start=1
     mat_peptide     4844..5863
                     /product="colonisation factor antigen e"
                     /gene="cfa e"
                     /codon_start=1
     CDS             complement(6389..6823)
                     /note="truncated CfaD-like protein with a stop codon and
                     frameshift deletions between bp 6029 and 6824."
                     /product="colonisation factor antigen d'"
                     /gene="cfa d'"
                     /codon_start=1
                     /translation="MDFKYTEEKEMIKINNIMIHIYTVLYTSNCIMDIYSEEEKITCS
                     SNRLVFLERGVNISVRIQKQILSEKPYIAFRLNGDILRHLKNALMIIYGMSKIDINDC
                     RNMSRKIIKTEVDKTLLDVLKNINSYDDSVFISSLIYLISKI"
BASE COUNT     2442 a   1129 c   1271 g   2332 t      1 others
ORIGIN
        1 cttctctgct ctgrctgaag gtctgcagcc tcatcaactg agtgataaac aatcaactaa
       61 aggagcactt atgagcgatg tattgttgtc tatggatacc tgtcaccagc tgctgcggat
      121 gctgaaaaca cgccagtcag agtagttgac gtctaatagt taggggttca gatgagtggc
      181 tcagcgtatg taccatttgc ttccatacgg atttaaacct caggcggaat aggtgataga
      241 ttgtaaatct attcgtcatt atgttaactc aatttcaata tgcaaggcgg catagtgtgg
      301 tgtcattttg ttaaaaactt tttaactttg tcgattttag caggccacaa acgacaaacc
      361 ttcccgaagc cggtacccta gtgcttttcc tgataaatcg cctgtcagac tgatgtacac
      421 ccaacctaac ggtgacatta agcattaatc ttcacgccta aaccttatgt gagaacggcg
      481 ttgtacaacc ttgtagtggc gatgagcatt acaacgaaaa ttatccacat agtgcgttgg
      541 gatatttctt attgaggaaa tatcggtgtc attaagtaac gttaacttaa gataaagaat
      601 ctgtctggaa atcgcagggc caagaacact cagtacatct gtggcgataa tactatcgct
      661 tcttattcat tccaatatgc agttcctgtg gatatttgct tgaacatcgc agcattagtg
      721 agtgcacaga attatagcat cgataccaaa taacacaggt tacacaatga tttgtttgca
      781 ttgtcttgtt tttttatctc attttttttt gttttgattt attattgatg gaagctcagg
      841 aggaaatatg cataaattat tctatttact aagtttactc atggctccat ttgttgcaaa
      901 tgcaaacttt atgatatatc caatatcaaa agatttaaag aatggaaata gcgagttagt
      961 tcgtgtttat tcaaaatcaa aagagataca atatataaaa atatatacaa agaagattat
     1021 taatcccggt acaactgaag aatataaggt tgatataccc aattgggatg gtgggcttgt
     1081 agtcactcct cagaaagtta tcctccctgc aggagcgagt aaatcaatac gtttaactca
     1141 atttaaaata ccaaaaaaag aggaagttta cagagtatat tttgaggcgg taaaaccaga
     1201 tagcaaagaa aatgtaattg ataataaaaa actaacaaca gagctatctg ttaatataat
     1261 ttatgcggct ctaattagat ctttaccaag tgaacaaaat atatcactaa atatttctag
     1321 aaatgcaaaa aaaaatataa ttatttataa taatgggaat gttagagcag gcgttaaaga
     1381 tatttatttt tgtaagtcat ctaatatcga tgataactgt gtaaaaaaag cgtataacaa
     1441 gaatatatat ccagaaaaag tcatttgata cgctggttaa taacaatttt tcttatgttt
     1501 tcattaaatt aaaccatgaa ggcatagaaa aagagcaagg gctaatacaa ttaaaggttc
     1561 cttgattact catctatata ctaaggagtt ctaatgaaat ttaaaaaaac tattggtgca
     1621 atggctctga ccacaatgtt tgtagcagtg agtgcttcag cagtagagaa aaatattact
     1681 gtaacagcta gtgttgatcc tgcaattgat cttttgcaag ctgatggcaa tgctctgcca
     1741 tcagctgtaa agttagctta ttctcccgca tcaaaaactt ttgaaagtta cagagtaatg
     1801 actcaagtac atacaaacga tgcaactaaa aaagtaattg ttaaacttgc tgatacacca
     1861 cagcttacag atgttctgaa ttcaactgtt caaatgccta tcagtgtgtc atggggagga
     1921 caagtattat ctacaacagc caaagaattt gaagctgctg ctttgggata ttctgcatcc
     1981 ggtgtaaatg gcgtatcatc ttctcaagag ttagtaatta gcgctgcacc taaaactgcc
     2041 ggtaccgccc caactgcagg aaactattca ggagtagtat ctcttgtaat gactttggga
     2101 tcctgattct tttatttaaa aggagggggt atgtttatac atccctcctt tttcatggaa
     2161 atgaatttat atgaagcata aaaaaaagaa tagattagtc gtagcgattt cagttgcatt
     2221 aattccatat ataggggtta ccggagatat acccgactct ttccgtgatt tatggggaga
     2281 acaagatgaa ttttatgaag taaaactata tgggcaaact ctaggaatac atcgaattaa
     2341 aacaacccca acacatatta agttttattc acccgaaagc attttagata aaataaattt
     2401 aaaaaaagaa aaggaaaagg aattgagtgt tttttttact aattcttttt caagaaatgg
     2461 caatatgagt tgtcagggta acactactat acagtataac tgcaattaca ttaaaacaaa
     2521 atcagtagat gtcatcgttg atgatgttga taatgttgtt aaccttttta taggtaatga
     2581 atttctggat tctgaagcac acaatgatga atatcatcaa ttatcacgga atgtaaaaaa
     2641 agcttttata caaagccaga caattaatct ctcagattct ggaaagtata aaagattgtc
     2701 tatttcaggg aatagcgcgc tgggtattac agatacaagt tatgctgtct taaattggtg
     2761 gatgaattac aataaatcta atggttacag caacaacgaa aaaacaatca atagtttata
     2821 ctttagacat gatttagata agagatatta ttatcaattt ggacgaatgg atcgtacaga
     2881 tttatcacaa agtattagcg ggagctttaa ttttaactta cttcctttac ccgatattga
     2941 tggtatacgg acaggaacca cacaatctta tatcaaaaat acagataagt ttatcgcatc
     3001 ccctgtaact gttatgttaa ctaatttttc cagagtggaa gcttttcgca atgatcaatt
     3061 attgggcgta tggtatttag attctggagt aaatgaatta gatacagctc gtttacctta
     3121 tggcagttac gatcttaaat taaaaatttt tgaaaacact caattagttc gtgaagaaat
     3181 aattcctttt aataaaggaa gaagctctat tggtgatatg caatgggata ttttcgttca
     3241 gggagggaat attgttaatg ataatgatcg ttacatagaa aaacaaaata atcataagtc
     3301 atcgattaat actgggctac gtttaccaat tacgaaaaat atctctgttc aacagggagt
     3361 atctgttata gataataaaa gttattatga aggaagtctg aaatggaatt ccggcattct
     3421 atctggctca ctaaatagtg agttcagttt tctttgggga gataatgcaa aaggtaatta
     3481 tcaaagtatc tcgtataccg atggatttag cttatcattt tatcataatg ataagcgggt
     3541 cgataattgt ggaagaaatt acaatgctgg ttggagtgga tgctacgaat catattcggc
     3601 atctttaagt attcctttat taggatggac aagtactctg ggatatagtg acacttatag
     3661 tgaatcagtg tataaaagcc atattctttc tgaatatggc ttttataatc aaaacatata
     3721 taaagggaga acccaaagat ggcaactgac ttcatccacc tctttaaaat ggatggatta
     3781 taattttatg ccagcaattg gaatatataa cagtgaacaa agacaactga ctgataaagg
     3841 cggatatata tctgtaacta tcacccgagc cagcagagaa aattcattaa atacagggta
     3901 ttcttacaac tattccagag gaaactattc ttctaacgaa ttatttgttg atggatatat
     3961 gacatcaaca aataatggtg attatcatga ggcaggaatg cgttttaata aaaatagaca
     4021 taatgcagaa ggtagacttt caggtcgtat aaacaatcga tttggagatt taaatggttc
     4081 attcagcatg aataaaaaca gaaacaccaa cagtaccaat cattctctca ctggtggtta
     4141 taattcctca tttgctctta caagtgatgg attttactgg ggaggaagta cagctggttt
     4201 gacaaaactg gctggcggta ttatcaaggt taaatcaaac gatactaaaa aaaacttggt
     4261 aaaagtgact gggacattgt acggtgatta ttcgctaggg agcaacgata atgcttttat
     4321 tcctgtacca gcattaactc cagccagttt aatcattgaa gataataatt atggtgataa
     4381 taatatttct atacttgcgc caacaaacaa cgatatgttt atgttgccgg gtaatgttta
     4441 tcctgttgaa attgaaacca aagtaagtgt ttcttatatt ggtagaggtt ttgacccaaa
     4501 cggcacgcca ctttctggcg cacatgtttt gaatgaacca catgttatcc tggatgagga
     4561 cggtggattt tcgtttgaat atacaggtaa tgagaaaaca ctttttttat taaagggcag
     4621 gactatttat acatgtcaac tggggaaaaa taaagttcat aaaggcattg ttttcgtcgg
     4681 ggatgttata tgtgatatta atagcacaag ttccttacca gatgaatttg taaagaaccc
     4741 acgtgtgcag gatttgctgg caaagaatga taaaggataa acgatgaata aaattttatt
     4801 tatttttaca ttgttttttt cttcagggtt ttttacattt gccgtatcgg cagataaaaa
     4861 tcccggaagt gaaaacatga ctaatactat tggtccccat gacagggggg gatcttcccc
     4921 catatataat atcttaaatt cctatcttac agcatacaat ggaagccatc atctgtatga
     4981 taggatgagt tttttatgtt tgtcttctca aaatacactg aatggagcat gcccaagcag
     5041 tgatgcccct ggcactgcta caattgatgg cgaaacaaat ataacattac aatttacgga
     5101 aaaaagaagt ctaattaaaa gagaactgca aattaaaggc tataaacaat ttttgttcaa
     5161 aaatgctaat tgcccatcta aactagcact taactcatct cattttcaat gtaatagaga
     5221 acaagcttca ggtgctactt tatcgttata cataccagct ggtgaattaa ataaattacc
     5281 ttttgggggg gtctggaatg ccgttctgaa gctaaatgta aaaagacgat atgatacaac
     5341 ctatgggact tacactataa acatcacagt taatttaact gataagggaa atattcagat
     5401 atggttacca cagttcaaaa gtaacgctcg tgtcgatctt aacttgcgtc caactggtgg
     5461 tggtacatat atcggaagaa attctgttga tatgtgcttt tatgatggat atagtactaa
     5521 cagcagctct ttggagataa gatttcagga tgataattct aaatctgatg gaaaatttta
     5581 tctaaagaaa ataaatgatg actccaaaga acttgtatac actttgtcac ttctcctggc
     5641 aggtaaaaat ttaacaccaa caaatggaca ggcattaaat attaacactg cttctctgga
     5701 aacaaactgg aatagaatta cagctgtcac catgccagaa atcagtgttc cggtgttgtg
     5761 ttggcctgga cgtttgcaat tggatgcaaa agtgaaaaat cccgaggctg gacaatatat
     5821 ggggaatatt aaaattactt tcacaccaag tagtcaaaca ctctagataa caacatattg
     5881 gcgctattgc cggccaatat tgtaaatagg taatctgttt gttaacaaaa cattttattt
     5941 caattcagtt tgcgtcgcaa taaatctcta ctagagacat ttttatacag catagtatta
     6001 tgcaacacat tcaaaataaa gtaattttta tcctccttta aaataagtaa aaaactgctt
     6061 tggtgcaaca ccataatgtt tattaaaaac cctaataaaa taagatatac tagaaattcc
     6121 aatcatattt gatatctgtg atatctggta tggattttca agcagcaata acgcagcctt
     6181 gctcattctc gattgcatta agatctggtt aaaattagta ttctcagatt ctagtctttt
     6241 cctgatggtt atttctgata cattaaacgt atctgcaata atagccaaag tccattttct
     6301 ggatagatct ttttcgataa cactcctgac cttatcagaa aaaaattcac agatgatata
     6361 taaattgatt ctattatttt tcattattct aaatttttga aatcaaatat atcagactag
     6421 atataaaaac tgagtcatca taactattta tattttttaa tacatccagt aaggttttat
     6481 ctacttctgt tttaattatt tttcttgaca tatttctaca atcattgata tctatttttg
     6541 acatgccata tattatcatt aatgcattct ttaaatgtct tagtatgtct ccgttcaatc
     6601 tgaatgcaat atatggtttt tctgataaaa tttgcttctg tattcttaca gatatattta
     6661 ctcctctttc aagaaataca agtctgttac tagaacatgt gattttctct tcttctgaat
     6721 atatatccat aatacaattt gatgtatata atacagtgta tatatgaatc ataatattat
     6781 taatttttat catttctttt tcttcagtgt atttaaaatc catcttatac ctacaaaagg
     6841 caagaggttt gatttgcttg tacaatcacc ttttgaacag ttggcctaac ttttgctaaa
     6901 agaaagatat gttttttcaa cttttttgct ttttttatta ttgtatcttt ttgatgcttt
     6961 tctgtgatgt acacttatgt gtgattaatg ttttatcgga gatagtgttt caatggaaat
     7021 aaaagagtgt tttcatgttt tatcaaaaaa agccgctctg gcagaaaaaa acatggattt
     7081 agtttctgca tttgaacttt ggaaacaagc atcacttttg tgtaaaaacg ctaaaaatat
     7141 agactggtgc ataaatagag caatgttttg tgaag
//