From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: B.taurus genes for lymphotoxin and tumor necrosis factor alpha
Date: 15 Aug 92 10:01:20 GMT
Distribution: bionet
Checksum: 56568    12

LOCUS       BOVTNFG      7229 bp ds-DNA             MAM       15-AUG-1992
DEFINITION  B.taurus genes for lymphotoxin and tumor necrosis factor alpha
ACCESSION   Z14137
KEYWORDS    TNF gene; lymphotoxin; tumor necrosis factor alpha.
SOURCE      Bos taurus DNA.
  ORGANISM  Bos taurus
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Artiodactyla; Ruminantia; Pecora; Bovidae; Bovinae;
            Bovini.
REFERENCE   1  (bases 1 to 7229)
  AUTHORS   Cludts,I.
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
REFERENCE   2  (bases 1 to 7229)
  AUTHORS   Cludts,I., Cleuter,Y., Burny,A. and Droogmans,L.
  TITLE     Cloning and characterization of the tandemly arranged bovine
            lymphotoxin and tumor necrosis factor-alpha genes.
  JOURNAL   Cytokine (1992) In press
  STANDARD  full automatic
COMMENT
            From EMBL    entry BTTNFG;  dated 02-AUG-1992.
FEATURES             Location/Qualifiers
     exon            <1213..1308
                     /number=1
     exon            <4189..4374
                     /number=1
     CDS             join(1213..1308,1396..1501,1714..2126)
                     /product="lymphotoxin"
                     /codon_start=1
                     /translation="MTPPGRLYLLRVCSTPPLLLLGLLLALPLEAQGLRGIGLTPSAA
                     QPAHQQLPTPFTRGTLKPAAHLVGDPSTQDSLRWRANTDRAFLRHGFSLSNNSLLVPT
                     SGLYFVYSQVVFSGRGCFPRATPTPLYLAHEVQLFSPQYPFHVPLLSAQKSVCPGPQG
                     PWVRSVYQGAVFLLTRGDQLSTHTDGISHLLLSPSSVFFGAFAL"
     CDS             join(4189..4374,4954..4999,5184..5231,5547..5968)
                     /product="tumor necrosis factor alpha"
                     /gene="TNF"
                     /codon_start=1
                     /translation="MSTKSMIRDVELAEEVLSEKAGGPQGSRSCLCLSLFSFLLVAGA
                     TTLFCLLHFGVIGPQREESPGGPSINSPLVQTLRSSSQASSNKPVAHVVADINSPGQL
                     RWWDSYANALMANGVKLEDNQLVVPADGLYLIYSQVLFRGQGCPSTPLFLTHTISRIA
                     VSYQTKVNILSAIKSPCHRETPEWAEAKPWYEPIYQGGVFQLEKGDRLSAEINLPDYL
                     DYAESGQVYFGIIAL"
     TATA_signal     767..772
     intron          1309..1395
                     /number=1
     exon            1396..1501
                     /number=2
     intron          1502..1713
                     /number=2
     exon            1714..>2126
                     /number=3
     polyA_signal    2742..2747
     TATA_signal     3967..3972
     intron          4375..4953
                     /number=1
     exon            4954..4999
                     /number=2
     intron          5000..5183
                     /number=2
     exon            5184..5231
                     /number=3
     intron          5232..5546
                     /number=3
     exon            5547..>5968
                     /number=4
     polyA_signal    6747..6752
BASE COUNT     1702 a   2071 c   1902 g   1554 t
ORIGIN
        1 gaattctgaa gcctgcagtg tagagaactt aggcagttac cacattcctt gatgctggaa
       61 tgtgtttgtt tggggttata taaatctgat cttccgagtc cacctaaggt tgggatgagg
      121 tggagggagt ccttcagtaa gatgagacct catcatacgt gagttcagag cctcatccct
      181 gagtgggatt ttccaaacca agacacttct aagagcagaa cggagctcca tgaatgtttc
      241 caccaggaca acaggcataa accaggatgt cccaggcagg agggtgtata atgcaacttc
      301 ttgatatgaa acccaccaga tgacctggaa aatacacaga ctgggacaag ttgacaagtg
      361 ttttgggaca aagacatggg gtatgagagg aggaaacatg cacacagaga aggctgtaat
      421 cagtcaaggg tgcagaggtg ttacacaaaa tcactcttca gggaaccggc ctccagccca
      481 cgccctagca gctccccttc tcctcctctg aatttactgt cccttctcta gaactcctaa
      541 gcctgacccc gctccctggc cctcccagcc cacggttccc ctgaccccac tccctttccc
      601 agaactcagt cgtctgagcc cccagcctgc ggttctgtcc taggcctcag cctttcctgc
      661 cttcgactga aacagcagca tcttctaagc cctgggggct tccccaggcc ccagccccgg
      721 cctagaaccc gcccgccgcc tgccacgctg ccactgccgc ttcctctata aagggaccca
      781 ggcgtccggg ccaaggggcc cccgcacagc aggtgagact ctcccagccc atctcctcgg
      841 gctgctgggg cggtggactc cttccccggg ggcgttcagt ccctcctctc acaccccagc
      901 ccctcctgca cctccagcca gggccagggc ttggtggggc tggttttggt tggttcccct
      961 ctctctgact ccatctgtca gtctctttct ctctgtctgt gtctaacgtt ctgagccctt
     1021 tctgttcccc tcctatctgt ctctgtctct ctccctctgc tcacctcggg gtttccctga
     1081 ttgcatcttg tcccccttct ctgtctgtca cgccgtgtgt ctctcggggt ggttgtctcc
     1141 accggcagga ggcccgttct tcctcatcgc gccccgcccc actcactgtc tctctccccg
     1201 caggttctcc ccatgacacc acctggacgt ctctacctcc tgagggtgtg cagcacccca
     1261 cccctcctcc tcctggggct gctgctggcc ctgccgctgg aggcccaggt gaggcagcag
     1321 gaccaagagg ggccgtgggg ggcggcccag cccaatcctg ggccttaaag cctgtctgac
     1381 tctcttctcc cctaggggct ccgtggcatt ggcctcacac cctcagctgc acagcctgcc
     1441 catcagcaac tcccgacgcc cttcacccgt ggcaccctca aacccgccgc tcaccttgtt
     1501 ggtaaacatc cacccgccct cccgacaagt agcccccagc cctcctcctg cccctttcag
     1561 agacccaata tccacccccc accagctcac cccactccca ctccctctgt cctcccagcc
     1621 atccccagga actcagtcca gcacctgttg ccttggggac tgagaccact gacccccaag
     1681 tccttaagta tcatcccctc tggctcttcc caggagaccc cagcacccag gactcgctgc
     1741 gctggagggc aaacacggac cgcgccttcc tccgccacgg cttctctctg agcaacaatt
     1801 ccctcctggt ccccaccagt ggcctgtact tcgtctactc ccaagtggtc ttctccggga
     1861 gaggctgctt ccccagggcc acccccaccc ctctctacct ggctcatgag gtccagctgt
     1921 tttcccccca gtaccccttc cacgtgcctc tcctcagcgc tcagaagtcc gtgtgcccag
     1981 ggccacaggg gccgtgggtg cgctcagtgt accagggggc tgtatttctg ctcacccggg
     2041 gagaccagct atccactcac acagatggca tctcccacct gctcctcagc cccagtagtg
     2101 tcttctttgg agccttcgct ctgtagaaac atccagaaag aaacaaatag gcttcaaggc
     2161 cttctcccca ttttgcctcc attctgacca attcaggggt caccacacct ctcctttgac
     2221 tattccaaca gtcttgagtc ttccccccgc ccggttctca gcacctggag cttccaaaga
     2281 aggaattcta ggcacaccag gggaccagac cttcctggac caccccagat gctcagctga
     2341 ggacttcaag cctgcctcga aatgcccacc cagatccctt cctcggccct gcccatctag
     2401 ggggcctcgt cctggacatg gagaggggag ctgacacatg agggagcttg ggtggatgac
     2461 taaaggcagg gaggggggct tatttatgaa gggaaaacaa ttaaattatt tatttatgga
     2521 gcatggagag aagggaataa tagcgagaca tcagaagaaa gagctaatac acccaagaga
     2581 tgacgagtga gagggcttag gcacaaggat gacccagtgg gagagagaaa gcgaggctct
     2641 gagggaccaa ggggttctgg aaggaagtga aaagctgtta agagccagcc gccacttaac
     2701 tagacacccc tcaaggagat gtctgaccct caatgaagcc aaataaactt ttgtctgaaa
     2761 ttctgtctgc ctgtatgtct gttggggagg ggagaattcc ccagatgtct ctgaggaatg
     2821 gaggaaagac aggaatcaga gaggcctgag gggctctggg gaaacacggt gggctcgcag
     2881 ggtggtgcag ccagctcgct gcaagggaac cagaggataa gctgagaaga cagaggcagg
     2941 tccaaggtct ggagaagtgg gggtcagggg ccccctgagg aatgggggtt acaggaggct
     3001 cctaaggagc actgacaaca ctaaagagtg ttcaggatga tgggggagga tcatggggcc
     3061 cccctggaag acatggccat gccagggccc cgagaaatgg gacaacctcc aaggctgggg
     3121 actagagaac accagaggca tttcaggaga cctggtcaca cacacaggag ctctcaaggg
     3181 cagtgctgtc tccaggaaac tggagggaga agggattctt tggggataca tggccataca
     3241 caggaactct gaaggggggg tctccgtgca aaagttgggg agtcacattc ccttggaaac
     3301 caagactgaa accagcctca gagtttcagc gagttcctgg tcagagtgaa aggagaagcc
     3361 ctgctgtgct ggagttcgtg aattcccagc gttactttca gcccctgggg ctgtcccagg
     3421 cttggacctg ccatccgccc ccagttcttg cagagcctca agcttccctt tctccagctc
     3481 ctctcactcc ccagggtccc aaacacacat ctcaggaccc agcacagctt cctctgagtt
     3541 ttctctccat gaaggactca gctttataaa gcccctccca tttctaattc tacccctaca
     3601 tgagtcctgt ctggaaatca gaaggaacag accacagaac tcactcccac ccctaaagga
     3661 acagaaacag tgggagctga ggggcagagg ggaaatgggt ccaaccctca gggtcccata
     3721 ttcacagcag taactggcct agagaagccc actcagaatc cgagcgggcg gagtgtagga
     3781 agtatccttg atgcctgggt gtccccaact ttccaaaccc ccgcccccgc gatggagaag
     3841 aaaccgagac agaaggtgta gggcccgcta ccgcttcctc cagatgagct catgggtttc
     3901 tccaccaagg aagttttccg ctggttgaat gagagccttt ccccgccctc ttctcacccc
     3961 acagcatata aatgcagctg tttgcacacc cagccaacag aagctcccag agcggggaca
     4021 ccaggggacc agccaggaga gagacaagca gctgcagaac cccctggaga taacctccca
     4081 gacaacacac ccccgagaga cagccaggca acttgctctc tctcacatac cctgccacaa
     4141 ggctctcctg tctcccgtct ggacttgaac ccttctgaaa aagacaccat gagcaccaaa
     4201 agcatgatcc gggatgtgga gctggcggag gaggtgctct ccgagaaagc agggggcccc
     4261 cagggctcca gaagttgctt gtgcctcagc ctcttctcct tcctcctggt tgcaggagcc
     4321 accacgctct tctgcctgct gcacttcggg gtaatcggcc cccagaggga agaggtgagt
     4381 ttctggccgg ccttggctca ttctcccacc cagagagaaa tgagagagaa agagggtgag
     4441 agatggggtg gagaaagagg tgtgttgatg gggaaagatg gggaggaaaa tagtggagaa
     4501 agatggggag gcaggaggag acatggagag agatggggga aaagggagaa gggtggagaa
     4561 ataacacttg gcacatgggg gatgccctct aaatatttgt tggacgaatg agtaagcaga
     4621 gatacagaca taaagagaga tgtgtagaca gataaggggt gtgagtagaa agatggagac
     4681 tcaaaacaag tgatctggat gaagatgatg agacagaaag agatacagac gataggaaag
     4741 agagataagg aaagatgaag ctaagtgtgt ctggcatgtg gaaggaactc aatgaaagag
     4801 ctgttaaatg gatgaatgaa caaaaaaaat ggatgaatgg agagagaaga caagacccat
     4861 cagggcagag agtacaagct agagaagcag ccagcagttt ctccttcagg ggtgacttgc
     4921 tctaacactc atcctccttt ttctccccag cagtccccag gtggcccctc catcaacagc
     4981 cctctggttc aaacactcag taagtgtctc cacaacctct cttctaattc tggacttggg
     5041 gggtgggtgt gggtggggtt tagtcaaggg atggaagcag taggggaaat ttagggtttg
     5101 ggatcggggg gagaaggaat ggaggttaaa gtatgggata ttttctggga agcttacctt
     5161 ttcctttcct cttttctcct cagggtcctc ttctcaagcc tcaagtaaca agccggtagc
     5221 ccacgttgta ggtaagagtt ctgaggatgt atctgggtca tgaagaagca gagagacagt
     5281 gcagggtagg attagggggc tgaatccaga ctgagggcag acagagacag tgtgagaaga
     5341 attcatgagc tcaaggggag ggtggtggat acttagaact tgagggggat actcagaacc
     5401 tcatggccag atgggatgtg ggaacacaga cagaggggac aggaaccgga tgtggggggc
     5461 gggcagaaca cgagggccag gatgtggaga gtagaactga cagggtcgca ctgatcaccc
     5521 ctccctgttc ttccccctcc ctccagccga catcaactct ccggggcagc tccggtggtg
     5581 ggactcgtat gccaatgccc tcatggccaa cggtgtgaag ctggaagaca accagctggt
     5641 ggtgcctgct gacgggcttt acctcatcta ctcacaggtc ctcttcaggg gccaaggctg
     5701 cccttccacc cccttgttcc tcacccacac catcagccgc attgcagtct cctaccagac
     5761 caaggtcaac atcctgtctg ccatcaagag cccttgccac agggagaccc cagagtgggc
     5821 tgaggccaag ccctggtatg aacccatcta ccagggagga gtcttccagc tggagaaggg
     5881 agatcgcctc agtgctgaga tcaacctgcc ggactacctg gactatgccg agtctgggca
     5941 ggtctacttt gggatcatcg ccctgtgagg gtgcaggaca tgcatcttct cccacctcag
     6001 ttaccttatt atttaatcct tcaggccctt ctcatcccct atctggttta gaaagggaat
     6061 tatgggctca gggctgggct ccaagcatcc aactttaaac aacagctaca tttagaaatt
     6121 agggatgtag ggaagtgagg cctggacaac gggccaccaa ccaccatcaa ggactggaac
     6181 tggaacttcc agaactccct ggggtccaca agcttgggtt cccggatgca acctgggaca
     6241 cccagaatgt gagggccagg gctcttaccg gaacacttcg caacattcct tgagaagatc
     6301 tcacctagaa cttgacatgc gtggacttca actctccctt cctgccaatg tttccagact
     6361 cccctgaggt gggaagccca gccccaaccc cactgggcca actccctctg tttatgtttg
     6421 cacttgtgat tatttattat ttatttatta tttatttatt tactaatgaa tgtatttatt
     6481 caggaggtca aggtgtcctg ggagacacaa actaagggct gccttggctc agatgtgttt
     6541 tctgtgaaaa cggagctgaa ctgcaggttg ctcccaccat gcctcctcgc ctttgtgcct
     6601 ccttttgctt atgtttttta aaaaatattt atctgatcga gttgtctaaa tgatgctgat
     6661 ttggtgactg atttgtcgct acatcactga acctccgctc cccaggggag tcatgcctgt
     6721 aaccgcccta ctggtcagtg gcgagaaata aagtgtcctg agaaaagaaa catgcctcta
     6781 tctggaaatt aattctgcat gggcctcttc ttgcgggtga agtgaagtga agtcgctcag
     6841 tcgtgcccga cactttgcga ccccatggac agtagcctgc accaagctcc tccatccatg
     6901 ggattttcta ggcaagagta ctggagtggg ttgccattgc cttctccagg gaatcttcct
     6961 gacccaggga tcgaacccag gtctcccgcc ttgtagatag acgctttacc gtctgagcca
     7021 ccagggaagt cctcttgggg gtaagaggct gctaaccgaa gttcttctcc acagggctta
     7081 agatccctgg acctcactcc ttgaacttct ggggctcaag agatcctacc taagacaaag
     7141 cccagctgaa tatgccccta cccaaagaaa ggaacttgaa cttaattacc tctcccttag
     7201 gacatgggaa tttccaactc tgggaattc
//