From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Trypanosoma brucei (clone WaTat1.12) surface glycoprotein (VSG)
Date: 24 Jul 92 10:10:30 GMT
Distribution: bionet
Checksum: 46987     6

LOCUS       TRBSURGLYB   1709 bp ss-mRNA            INV       24-JUL-1992
DEFINITION  Trypanosoma brucei (clone WaTat1.12) surface glycoprotein (VSG)
            mRNA, complete cds.
ACCESSION   M83695
KEYWORDS    surface glycoprotein.
SOURCE      Trypanosoma brucei cDNA to mRNA.
  ORGANISM  Trypanosoma brucei
            Eukaryota; Animalia; Protozoa; Sarcomastigophora; Mastigophora;
            Kinetoplastida; Trypanosomatina; Trypanosomatidae.
REFERENCE   1  (bases 1 to 1709)
  AUTHORS   Barbet,A.F., Davis,W.C. and McGuire,T.C.
  TITLE     Cross-neutralization of two different trypanosome populations
            derived from a single organism
  JOURNAL   Nature 300, 453-456 (1982)
  STANDARD  full automatic
REFERENCE   2  (bases 1 to 1709)
  AUTHORS   Barbet,A.F., Myler,P.J., Williams,R.O. and McGuire,T.C.
  TITLE     Shared surface epitopes among trypanosomes of the same serodeme
            expressing different variable surface glycoprotein genes
  JOURNAL   Mol. Biochem. Parasitol. 32, 191-200 (1989)
  STANDARD  full staff_review
REFERENCE   3  (bases 1 to 1709)
  AUTHORS   Kamper,S.M. and Barbet,A.F.
  TITLE     Surface epitope variation via mosaic gene formation is potential
            key to long-term survival of Trypanosoma brucei.
  JOURNAL   Mol. Biochem. Parasitol. 53, 33-44 (1992)
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     sig_peptide     86..169
                     /standard_name="VSG"
                     /product="surface glycoprotein"
                     /gene="VSG"
                     /evidence=EXPERIMENTAL
                     /codon_start=1
                     /citation=[2]
                     /citation=[3]
     CDS             86..1624
                     /standard_name="VSG"
                     /product="surface glycoprotein"
                     /gene="VSG"
                     /evidence=EXPERIMENTAL
                     /codon_start=1
                     /translation="MTKSPTKLSQVALSYLLLIAAAPSHVQPASQSALDKGLWEGICK
                     ATAELNKVPGTMTHELTQILQKAKTMRRGELKAQIFGLRHAGTELAKRAAVMAAYFGR
                     RHNQMLATLETKDIEKAIDAAAKAAYLQGRIDDPLHLLAAVDNTNNLCLSTTGATGNK
                     PAKTPGKLGDVDCPLSAPSAKNKAAELSGITEAGFTMLKTDVTSGNAKQVASGAKKCN
                     LLSSVNTQGFGETEAAISTHHEILGGYITIKNTNTEIDVTAHNKAHTVESGKHESWTA
                     AHAAIMVSPKHTTTPYINETGEIHSRSDMTKVLQAVYSAKPPITQTDLENKVIEIFQG
                     KEAKKLQDYEDAVNQVTIPAGIAGLETDQTLAAVNDEDKLIAIRAFYEQQVSAAYGKM
                     VATISEASNKQTATAPADCGKKLKKVDCKESDGCKWNSTDKSEGEFCKPKGEDEQKAQ
                     GAGTGDGAAGEQKKEDKCTGKKKDDCKDGCKWEGKECKDSSILATKKFALSVVSAAFV
                     ALLF"
                     /citation=[2]
                     /citation=[1]
                     /citation=[3]
     mat_peptide     170..1552
                     /standard_name="VSG"
                     /product="surface glycoprotein"
                     /gene="VSG"
                     /evidence=EXPERIMENTAL
                     /codon_start=1
                     /citation=[2]
                     /citation=[3]
BASE COUNT      624 a    391 c    404 g    290 t
ORIGIN
        1 aacgctatta ttagaacagt ttctgtacta tattgaagag aaaaaaggag tcaagaaaca
       61 tagaaaagct agccatcaaa agaagatgac aaaaagtcca acgaaactga gccaagtagc
      121 gctttcatac ttgttactaa tcgccgcagc accgagtcat gtacaacccg ctagtcaatc
      181 agccttggac aaagggctct gggagggcat atgcaaagcg acggcagagc tgaacaaagt
      241 tcccgggacg atgacgcacg aattgacgca gatcctccaa aaagcgaaga cgatgcggcg
      301 tggagaactg aaagcacaaa tatttgggct aaggcatgca ggaacagaat tggccaagag
      361 agcagcggta atggcggcat atttcggccg taggcacaat cagatgcttg caacgcttga
      421 aacgaaagac atcgaaaaag ccatcgacgc ggcagccaaa gcagcgtacc ttcaaggcag
      481 aatagacgac ccgcttcatt tactggcagc tgtggataac acaaacaatc tttgtctgtc
      541 tacgaccgga gcgacaggca acaaaccggc aaagacgccc ggcaaactgg gcgacgtcga
      601 ctgcccacta tcagcgccgt cagcaaaaaa caaggcagct gaattaagcg gtataacaga
      661 ggccggcttt accatgctaa aaacggatgt gaccagcggc aacgcaaaac aggtcgcgag
      721 cggcgcaaag aaatgcaacc ttctaagttc agtaaacacc caaggcttcg gagagacaga
      781 ggcggcgatc agcacccacc acgaaattct cggaggatac ataacaataa aaaacacaaa
      841 cactgaaata gacgtaaccg cgcacaacaa agcacacaca gtcgaaagcg gcaagcacga
      901 aagctggacg gcggcacatg cggccattat ggtgagccca aaacacacaa cgacacccta
      961 tatcaatgaa actggggaaa ttcacagcag gtcagacatg acaaaagtgt tgcaagccgt
     1021 ttacagcgca aagccgccca taacccaaac tgacctagag aacaaggtca tagaaatatt
     1081 tcaaggaaaa gaagcaaaga aactgcaaga ctacgaagac gctgtaaacc aagtaacgat
     1141 accagccggc atagcaggac tggaaactga ccagacgcta gcagcagtaa acgacgaaga
     1201 caaactaatc gcaatcagag ccttttatga acagcaggtc agcgcagcct atggcaaaat
     1261 ggtagcgacg atatccgaag caagcaataa acaaacagcc accgccccag cagactgcgg
     1321 taaaaaacta aagaaagtag actgcaaaga gagcgatgga tgcaagtgga atagcactga
     1381 caaaagtgaa ggagaattct gcaaacctaa aggggaagat gaacaaaaag cccaaggagc
     1441 aggaacagga gatggagctg caggagaaca aaagaaagag gataaatgca cagggaagaa
     1501 aaaggatgac tgtaaagatg gatgcaaatg ggaaggaaaa gaatgcaaag attcctctat
     1561 tctagcaact aaaaaattcg ccctcagcgt ggtttctgct gcctttgtgg ccttgctttt
     1621 ttaaaacaaa ttttttccct ctctttttaa aattttcctt tgctaaaatt cttgctactt
     1681 gaaaaacttt ctgatatatt ttaacacct
//