From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: E.coli metH gene encoding methionine synthase, complete cds.
Date: 27 Jan 93 05:47:25 GMT
Distribution: bionet
Checksum: 13702 8

LOCUS       ECOMETH      3828 bp ds-DNA             BCT       26-JAN-1993
DEFINITION  E.coli metH gene encoding methionine synthase, complete cds.
ACCESSION   J04975
KEYWORDS    metH gene.
SOURCE      E.coli (strain K12) DNA.
  ORGANISM  Escherichia coli
            Prokaryotae; Gram-negative facultatively anaerobic rods;
            Enterobacteriaceae.
REFERENCE   1  (bases 1 to 3828)
  AUTHORS   Banerjee,R.V., Johnston,N.L., Sobeski,J.K., Datta,P. and
            Matthews,R.G.
  TITLE     Cloning and sequence analysis of the Escherichia coli metH gene
            encoding cobalamin-dependent methionine synthase and isolation of a
            tryptic fragment containing the cobalamin-binding domain
  JOURNAL   J. Biol. Chem. 264, 13888-13895 (1989)
  STANDARD  full automatic
COMMENT     Computer readable copy of sequence [1] kindly submitted by
            R.V.Banerjee, 07-JUL-1989.
FEATURES             Location/Qualifiers
     CDS             220..3594
                     /note="methionine synthase;  (EC 2.1.1.13)"
                     /codon_start=1
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARRCADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDGLV
                     AAYRESTKALVEGGEDLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGEDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKTDDTANAQQAEWRSWEVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHEIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRTAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTAFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWAMRRTRTSATKS"
BASE COUNT      917 a    992 c   1107 g    812 t
ORIGIN      About 91 min. on K12 map.
        1 gcgggaatgg gtgcgaccat gacagtctct tttttctgta tcgtggaaat cattttcatt
       61 tttattgtta gctaatgcaa tagttactga actgatccga tgagttaatg ttgaacaaat
      121 ctcatgttgc gtggtggtcg cttttaccac agatgcgttt atgccagtat ggtttgttga
      181 atttttatta aatctgggtt gagcgtgtcg ggagcaagtg tgagcagcaa agtggaacaa
      241 ctgcgtgcgc agttaaatga acgtattctg gtgctggacg gcggtatggg caccatgatc
      301 cagagttatc gactgaacga agccgatttt cgtggtgaac gctttgccga ctggccatgc
      361 gacctcaaag gcaacaacga cctgctggta ctcagtaaac cggaagtgat cgccgctatc
      421 cacaacgcct actttgaagc gggcgcggat atcatcgaaa ccaacacctt caactccacg
      481 accattgcga tggcggatta ccagatggaa tccctgtcgg cggaaatcaa ctttgcggcg
      541 gcgaaactgg cgcgacgttg tgctgacgag tggaccgcgc gcacgccaga gaaaccgcgc
      601 tacgttgccg gtgttctcgg cccgaccaac cgcacggcgt ctatttctcc ggacgtcaac
      661 gatccggcat ttcgtaatat cacttttgac gggctggtgg cggcttatcg agagtccacc
      721 aaagcgctgg tggaaggtgg cgaggatctg atcctgattg aaaccgtttt cgacaccctt
      781 aacgccaaag cggcggtatt tgcggtgaaa acggagtttg aagcgctggg cgttgagctg
      841 ccgattatga tctccggcac catcaccgac gcctccgggc gcacgctctc cgggcagacc
      901 accgaagcat tttacaactc attgcgccac gccgaagctc tgacctttgg cctgaactgt
      961 gcgctggggc ccgatgaact gcgccagtac gtgcaggagc tgtcacggat tgcggaatgc
     1021 tacgtcaccg cgcacccgaa cgccgggcta cccaacgcct ttggtgagta cgatctcgac
     1081 gccgacacga tggcaaaaca gatacgtgaa tgggcgcaag cgggttttct caatatcgtc
     1141 ggcggctgct gtggcaccac gccacaacat attgcagcga tgagtcgtgc agtagaagga
     1201 ttagcgccgc gcaaactgcc ggaaattccc gtagcctgcc gtttgtccgg cctggagccg
     1261 ctgaacattg gcgaagatag cctgtttgtg aacgtgggtg aacgcaccaa cgtcaccggt
     1321 tccgctaagt tcaagcgcct gatcaaagaa gagaaataca gcgaggcgct ggatgtcgcg
     1381 cgtcaacagg tggaaaacgg cgcgcagatt atcgatatca acatggatga agggatgctc
     1441 gatgccgaag cggcgatggt gcgttttctc aatctgattg ccggtgaacc ggatatcgct
     1501 cgcgtgccga ttatgatcga ctcctcaaaa tgggacgtca ttgaaaaagg tctgaagtgt
     1561 atccagggca aaggcattgt taactctatc tcgatgaaag agggcgtcga tgcctttatc
     1621 catcacgcga aattgttgcg tcgctacggt gcggcagtgg tggtaatggc ctttgacgaa
     1681 cagggacagg ccgatactcg cgcacggaaa atcgagattt gccgtcgggc gtacaaaatc
     1741 ctcaccgaag aggttggctt cccgccagaa gatatcatct tcgacccaaa catcttcgcg
     1801 gtcgcaactg gcattgaaga gcacaacaac tacgcgcagg actttatcgg cgcgtgtgaa
     1861 gacatcaaac gcgaactgcc gcacgcgctg atttccggcg gcgtatctaa cgtttctttc
     1921 tcgttccgtg gcaacgatcc ggtgcgcgaa gccattcacg cagtgttcct ctactacgct
     1981 attcgcaatg gcatggatat ggggatcgtc aacgccgggc aactggcgat ttacgacgac
     2041 ctacccgctg aactgcgcga cgcggtggaa gatgtgattc ttaatcgtcg cgacgatggc
     2101 accgagcgtt tactggagct tgccgagaaa tatcgcggca gcaaaaccga cgacaccgcc
     2161 aacgcccagc aggcggagtg gcgctcgtgg gaagtgaata aacgtctgga atactcgctg
     2221 gtcaaaggca ttaccgagtt tatcgagcag gataccgaag aagcccgcca gcaggctacg
     2281 cgcccgattg aagtgattga aggcccgttg atggacggca tgaatgtggt cggcgacctg
     2341 tttggcgaag ggaaaatgtt cctgccacag gtggtcaaat cggcgcgcgt catgaaacag
     2401 gcggtggcct acctcgaacc gtttattgaa gccagcaaag agcagggcaa aaccaacggc
     2461 aagatggtga tcgccaccgt gaagggcgac gtccacgaga tcggtaaaaa tatcgttggt
     2521 gtggtgctgc aatgtaacaa ctacgaaatt gtcgatctcg gcgttatggt gcctgcggaa
     2581 aaaattctcc gtaccgctaa agaagtgaat gctgatctga ttggcctttc ggggcttatc
     2641 acgccgtcgc tggacgagat ggttaacgtg gcgaaagaga tggagcgtca gggcttcact
     2701 attccgttac tgattggcgg cgcgacgacc tcaaaagcgc acacggcggt gaaaatcgag
     2761 cagaactaca gcggcccgac ggtgtatgtg cagaatgcct cgcgtaccgt tggtgtggtg
     2821 gcggcgctgc tttccgatac ccagcgtgat gattttgtcg ctcgtacccg caaggagtac
     2881 gaaaccgtac gtattcagca cgggcgcaag aaaccgcgca caccaccggt cacgctggaa
     2941 gcggcgcgcg ataacgattt cgcttttgac tggcaggctt acacgccgcc ggtggcgcac
     3001 cgtctcggcg tgcaggaagt cgaagccagc atcgaaacgc tgcgtaatta catcgactgg
     3061 acaccgttct ttatgacctg gtcgctggcc gggaagtatc cgcgcattct ggaagatgaa
     3121 gtggtgggcg ttgaggcgca gcggctgttt aaagacgcca acgacatgct ggataaatta
     3181 agcgccgaga aaacgctgaa tccgcgtggc gtggtgggcc tgttcccggc aaaccgtgtg
     3241 ggcgatgaca ttgaaatcta ccgtgacgaa acgcgtaccc atgtgatcaa cgtcagccac
     3301 catctgcgtc aacagaccga aaaaacagcg ttcgctaact actgtctcgc tgacttcgtt
     3361 gcgccgaagc tttctggtaa agcagattac atcggcgcat ttgccgtgac tggcgggctg
     3421 gaagaggacg cactggctga tgcctttgaa gcgcagcacg atgattacaa caaaatcatg
     3481 gtgaaagcgc ttgccgaccg tttagccgaa gcctttgcgg agtatctcca tgagcgtgtg
     3541 cgtaaagtct actgggctat gcgccgaacg agaacctcag caacgaagag ctgatccgcg
     3601 aaaactacca gggcatccgt ccggcaccgg gctatccggc ctgcccggaa catacggaaa
     3661 aagccaccat ctgggagctg ctggaagtgg aaaaacacac tggcatgaaa ctcacagaat
     3721 ctttcgccat gtggcccggt gcatcggttt cgggttggta cttcagccac ccggacagca
     3781 agtactacgc tgtagcacaa acttcagcgc gatcaggttg aagattat
//