From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human mRNA for 2-oxoglutarate dehydrogenase, complete cds.
Date: 5 Nov 92 23:09:18 GMT
Distribution: bionet
Checksum: 36992 9

LOCUS       HUM2OGDH     4141 bp ss-mRNA            PRI       17-OCT-1992
DEFINITION  Human mRNA for 2-oxoglutarate dehydrogenase, complete cds.
ACCESSION   D10523 D90499
KEYWORDS    2-oxoglutarate dehydrogenase.
SOURCE      Homo sapiens (library: lamda gt11) cDNA to mRNA.
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 4141)
  AUTHORS   Koike,K., Urata,Y. and Goto,S.
  TITLE     cloning and nucleotide sequence of the cDNA encoding human
            2-oxoglutarate dehydrogenase (lipoamide)
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 89, 1963-1967 (1992)
  STANDARD  full automatic
COMMENT     Data kindly submitted in computer readable form by: Kichiko Koike
            Department of Pathological Biochemistry Atomic Disease Institute
            Nagasaki University School of Medicine 12-4 Sakamoto-machi
            Nagasaki-shi 852 Japan Phone:  0958-47-2111 Fax:    0958-47-8514.
FEATURES             Location/Qualifiers
     sig_peptide     59..178
                     /codon_start=1
     mat_peptide     179..3067
                     /EC_number="1.2.4.2"
                     /product="2-oxoglutarate dehydrogenase"
                     /codon_start=1
     polyA_site      4141
     CDS             59..3070
                     /product="2-oxoglutarate dehydrogenase precursor"
                     /codon_start=1
                     /translation="MFHLRTCAAKLRPLTASQTVKTFSQNRPAAARTFQQIRCYSAPV
                     AAEPFLSGTSSNYVEEMYCAWLENPKSVHKSWDIFFRNTNAGAPPALLPESPSLSRGS
                     LAAVAHAQSLVEAQPNVDKLVEDHLAVQSLIRAYQIRGHHVAQLDPLGILDADLDSSV
                     APLTLSHPQTNLGSMAWMSLTSTRSSTCPPTTFIGGQESALPLREIIRRLEMAYCQHI
                     GVEFMFINDLEQCQWIRQKFETPGIMQFTNEEKRTLLARLVRSTRFEEFLQRKWSSEK
                     RFGLEGCEVLIPALKTIIDKSSENGVDYVIMGMPHRGRLNVLANVIRKELEQIFCQFD
                     SKLEAADEGLRRCEVPPGHVSPQDQSCHRPGTLPCPWWPNPSHLEAADPVVMGKTKAE
                     QFYCGDTEGKKVMSILLHGDAAFAGQGIVYETFHLSDLPSYTTHGTVHVVVNNQIGFT
                     TDPRMARSSPYPTDVARVVNAPIFHVNSDDPEAVMYVCKVAAEWRSTFHKDVVVDLVC
                     YRRNGHNEMDEPMFTQPLMYKQIRKQKPVLQKYAELLVSQGVVNQPEYEEEISKYDKI
                     CEEAFARSKDEKILHIKHWLDSPWPGFFTLDGQPRSMSCPSTGLTEDILTHIGNVASS
                     VPVENFTIHGGLSRILKTRGEMVKNRTVDWALAEYMAFGSLLKEGIHIRLSGQDVERG
                     TFSHRHHVLHDQNVDKRTCIPMNHLWPNQAPYTVCNSSLSEYGVLGFGAGLRMASPNA
                     LVLWEAQFGDFHNTAQCIIDQFICPGQAKWVRQNGIVLLLPLGMEGMGPEHSSGPPRA
                     FLQMCNDDPDVLPDLKEANFDINQLYDCNWVVVNCSTPGNFFHVLRRQILLPFRKPLI
                     IFTPKSLLRHPEARSSFDEMLPGTHFQRVIPEDGPAAQNPENVKRLLFCTGKVYYDLT
                     RERKARDMVGQVAITRIEHVSPFPFDLLLKEVQKYPNAELAWCQEEHKNQGYYDYVKP
                     RLRTTISRAKPVWYAGRDPAAAPRTGNKKTH"
BASE COUNT      899 a   1197 c   1154 g    891 t
ORIGIN      
        1 cgggttcggg tggagctgag ccggagacag gcaattgtgg aaaaacttca ggacaaaaat
       61 gtttcattta aggacttgtg ctgctaagtt gaggccattg acggcttccc agactgttaa
      121 gacattttca caaaacagac cagcagcagc taggacattt caacagattc ggtgctattc
      181 tgcacctgtt gctgctgagc cctttctcag tgggactagt tcgaactatg tggaggagat
      241 gtactgtgct tggctggaaa accccaaaag tgtacataag tcatgggaca ttttttttcg
      301 caacacgaat gccggagccc caccggcact gctaccagag tccccttccc tgagccgagg
      361 ctccctggct gctgtggccc atgcacagtc cctggtagaa gcacagccca acgtggacaa
      421 gctcgtggag gaccacctgg cagtgcagtc actcatcagg gcatatcaga tacgagggca
      481 ccatgtagca cagctggacc ccctggggat tttggatgct gatctggact cctccgttgc
      541 cccgctgaca ttatctcatc cacagacaaa cttgggttct atggcctgga tgagtctgac
      601 ctcgacaagg tcttccactt gcccacccac cactttcatc gggggacagg aatcagcact
      661 tcctctgcgg gagatcatcc gtcggctgga gatggcctac tgccagcata ttggggtgga
      721 gttcatgttc atcaatgacc tggagcagtg ccagtggatc cggcagaagt ttgagacccc
      781 tgggatcatg cagttcacaa atgaggagaa acggaccctg ctggccaggc ttgtgcggtc
      841 caccaggttt gaggagttcc tacagcggaa gtggtcctct gagaagcgct ttggtctaga
      901 aggctgcgag gtactgatcc ctgccctcaa gaccatcatt gacaagtcta gtgagaatgg
      961 cgtggactac gtgatcatgg gcatgccaca cagagggcgg ctgaacgtgc ttgcaaatgt
     1021 catcaggaag gagctggaac agatcttctg tcaattcgat tcaaagctgg aggcagctga
     1081 tgaggggctc cggagatgtg aagtaccacc tgggcatgta tcaccgcagg atcaatcgtg
     1141 tcaccgacca ggaacattac cttgtccttg gtggccaaac ccttcccacc ttgaggccgc
     1201 tgaccccgtg gtgatgggca agaccaaagc cgaacagttt tactgtggcg acactgaagg
     1261 gaaaaaggtc atgtccatcc tgttgcatgg ggatgctgca tttgctggcc agggcattgt
     1321 gtacgagacc ttccacctca gcgacctgcc atcctacaca actcatggca ccgtgcacgt
     1381 ggtcgtcaac aaccagatcg gcttcaccac cgaccctcgg atggcccgct cctcccccta
     1441 ccccactgac gtggcccgag tggtgaatgc ccccattttc cacgtgaact cagatgaccc
     1501 cgaggctgtc atgtacgtgt gcaaagtggc ggccgagtgg aggagcacct tccacaagga
     1561 cgtggttgtc gatttggtgt gttaccggcg caacggccac aacgagatgg atgagcccat
     1621 gttcacgcag ccgctcatgt acaagcagat ccgcaagcag aagcctgtgt tacagaagta
     1681 cgctgagctg ctggtgtcgc agggtgtggt caaccagcct gagtatgagg aggaaatttc
     1741 caagtatgat aagatctgtg aggaagcttt tgccagatct aaagatgaga agatcttgca
     1801 cattaagcac tggctggact ctccctggcc tggcttcttc accctggacg ggcagcccag
     1861 gagcatgtcc tgcccctcca cgggtctgac ggaggatatt ctgacacaca tcgggaatgt
     1921 ggctagttct gtgcctgtgg aaaactttac tattcatgga gggctgagcc ggatcttgaa
     1981 gactcgtggg gaaatggtga agaaccggac tgtggactgg gctctagcgg agtacatggc
     2041 gtttggctcg ctcctgaagg agggcatcca cattcggctg agcggccagg acgtggagcg
     2101 gggcacattc agccaccgcc accatgtgct ccatgaccag aatgtggaca agagaacctg
     2161 catccccatg aaccatctct ggcccaatca ggccccctat actgtgtgca acagctcact
     2221 gtctgagtac ggcgtgctgg gctttggagc tgggcttcgc atggccagtc ctaatgccct
     2281 ggtcctctgg gaagcccaat ttggtgactt ccacaacacg gcccagtgta tcatcgacca
     2341 gttcatctgc ccgggacaag ccaagtgggt gcggcagaat ggcatcgtgt tgctgctgcc
     2401 cctaggcatg gagggcatgg gtccagaaca ttcctccggg ccgcccagag cgttcttgca
     2461 gatgtgcaac gatgacccag atgtcctgcc agaccttaaa gaagccaact tcgacatcaa
     2521 tcagctatat gactgcaatt gggttgttgt caactgctcc actcctggca acttcttcca
     2581 cgtgctacga cgccagatcc tgctgccatt ccggaagccg ttaattatct tcacccccaa
     2641 atccctgttg cgccaccccg aggccagatc cagctttgat gagatgcttc caggaaccca
     2701 cttccagcgg gtgatcccag aagatggccc tgcagctcag aacccagaaa atgtcaaaag
     2761 gcttctcttc tgcaccggga aagtgtatta tgacctcacc cgggagcgca aagcacgcga
     2821 catggtgggg caggtggcca tcacaaggat tgagcacgtg tcgccattcc cctttgacct
     2881 cctgctgaag gaggtgcaga agtaccccaa tgctgagctg gcctggtgcc aggaggagca
     2941 caagaaccaa ggctactatg actacgtgaa gccaagactt cggaccacca tcagccgcgc
     3001 caagcccgtc tggtatgccg gccgggaccc agcggctgct ccacgcaccg gcaacaagaa
     3061 gacccactga cggagctgca gcgcctcctg gacacggcct tcgacctgga cgtcttcaag
     3121 aacttctcgt agatgctgcc tagggttgct tgccactgcc ctctccacac ccatgactgc
     3181 cccttgcttc tcaactaaag aatagtgctt cagcgctgcc cacaccaccg cctcctcctc
     3241 gctgtgccac cacccctccc tctgctctca taggagttag gctgtcgtcc ccctccagtg
     3301 cttggctgcc ccacaggcca cacgctgccc aggctctgct gacttctgag cagttttcca
     3361 ggaggccggg gggagcagga ggaggaaagg tagcccccga gggatgtcct tggggagggg
     3421 tcagcctctg gcccacaatc ctccccacca gtctcaccca ctaggatagg aactgggcct
     3481 tgtgtgctgg cttccgctgt cacccagcaa ggcacaggct cctgtatttg agactaggat
     3541 agcttcatct tgagcctgag ccttagaatc tgtagaggag cctggagtcg gagtcggatc
     3601 tagccatggc tgggagaggg gagtctaggg tgggccccag ccgtggcgtg aactgaggat
     3661 gaacccgggg gcaggcttgg gcacggagag agccttggcc tgacctggga acagaaaggg
     3721 cagcttcagt ctctgcagtg tccattatct gctgttcctt cgagggttcc aggctgtgtg
     3781 tggggcccaa gcatgcccca cccaccctcc tgggcccagg cagcacctgg agcccacaga
     3841 gtctgtgtgt agccaggaag ccccgctcag gtagccaccg ccggggcact ggctgctctg
     3901 tcttggtcct gttaaccctc cacctcctct cttggactcc ctccccaccc caaccactct
     3961 ttctttctcc tttaacccaa tggagacttt ctgatgcatc gttttctttg ctgtgccaaa
     4021 gcaggtcaga agagggagag gagggggctg ggggtgaggg gccagccatg gccaaggcca
     4081 gctgcccctc atttatcact ctgaccttca cagggacaga tctgatttat ttattttggt
     4141 t
//