From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Hamster mevalonate transporter mRNA, complete cds.
Date: 29 Dec 92 19:49:22 GMT
Distribution: bionet
Checksum: 17263 7

LOCUS       CRUMEVTRSP   3345 bp ss-mRNA            ROD       09-DEC-1992
DEFINITION  Hamster mevalonate transporter mRNA, complete cds.
ACCESSION   M97382
KEYWORDS    mevalonate transporter.
SOURCE      Cricetulus sp. cDNA to mRNA.
  ORGANISM  Cricetulus sp.
            Eukaryota; Animalia; Chorqata; Vertebrata; Mammalia; Theria;
            Eutheria; Rodentia; Myomorpha; Cricetidae; Cricetinae; Cricetini.
REFERENCE   1  (bases 1 to 3345)
  AUTHORS   Kim,C.M., Goldstein,J.L. and Brown,M.S.
  TITLE     cDNA cloning of Mev, a mutant protein that facilitates cellular
            uptake of mevalonate, and identification of the point mutation
            responsible for its gain of function
  JOURNAL   J. Biol. Chem. 267, 23113-23121 (1992)
  STANDARD  full automatic
COMMENT     *cell line = Hamster (Chinese) ovary met-18b-2.
FEATURES             Location/Qualifiers
     5'UTR           18..211
     mutation        replace(1290,"")
                     /note="'t' in mutation"
     3'UTR           1697..3320
     CDS             212..1696
                     /product="mevalonate transporter"
                     /codon_start=1
                     /translation="MPPAIGGPVGYTPPDGGWGWAVVVGAFISIGFSYAFPKSITVFF
                     KEIEGIFNATTSEVSWISSIMLAVMYAGGPISSVLVNKYGSRPVMIAGGCLSGCGLIA
                     ASFCNTVQELYLCIGVIGGLGLAFNLNPALTMIGKYFYKKRPLANGLAMAGSPVFLST
                     LAPLNQAFFGIFGWRGSFLILGGLLLNCCVAGSLMRPIGPKPGKIEKLKSKESLQEAG
                     KSEANTDLMGGSPKGEKRSVLQTINKFLDLSLFAHRGFLLYLSGNVVMFFGLFTPLVF
                     LSNYGKSQHYSSEKSAFLLSILAFVDMVARPSMGLAANTKWIRPRIQYFFAASVVANG
                     VCHLLAPLSTSYIGFCIYAGVFGFACGWLSSVLFETLMDLVGPQRFSSAVGLVTIVEC
                     CPVLLGPPLLGRLNDMYGDYKYTYWACGVILIIAGIYLFIGMGINYRLVAKEQKAEEK
                     QKQEEGKEDDTSTDVDEKPKELTKATESPQQNSSGDPAEEESPV"
BASE COUNT      886 a    678 c    768 g   1013 t
ORIGIN      
        1 gtcgacccac gcgtccggcg acgtgactgg tcggccgtgt gggtgctgca gccaacgaac
       61 ccggcggcgg cggcggcaag ggaagcgagc gggaccccgg gctccgagga actgcggccc
      121 gcgccgccgc gtcacgcacc ctctgggcgc cgcgagacac acataacgat aatagatttg
      181 cattgcattt tgggattcat ctacacttaa aatgccacct gcaattggag gaccagttgg
      241 atataccccc ccagatggag gctgggggtg ggcagtggta gttggagctt tcatttctat
      301 tggcttctct tatgcatttc ccaaatccat caccgtcttc tttaaagaaa ttgaaggtat
      361 attcaatgct actaccagtg aagtgtcatg gatatcatcc atcatgttgg ctgtcatgta
      421 tgctggaggt cctatcagca gtgtcctggt gaataaatat ggcagccgtc cagtaatgat
      481 cgctggtgga tgcttgtctg gttgtggctt gattgcagct tctttctgta acacagtgca
      541 ggaactttac ttgtgcatcg gtgtcattgg aggtcttggg cttgctttca acttgaaccc
      601 agctctgacc atgattggca aatatttcta caagaagcga ccattggcca atgggctggc
      661 catggcaggc agccctgtgt tcctctctac cctggctcca cttaatcagg ctttctttgg
      721 tatttttggc tggagaggaa gcttcctaat tcttggtggc ctcctactaa actgttgtgt
      781 agctggatcc ctgatgcggc caatagggcc taagccaggc aagatagaaa aactaaagtc
      841 caaagaatct cttcaggaag ctggaaaatc tgaggcaaac acagatctca tgggaggaag
      901 ccccaaagga gaaaagcgat cagtcttaca gacaattaat aagttcctgg acctgtccct
      961 gtttgcccac agaggctttt tgctgtacct gtctgggaat gtagtcatgt tttttggact
     1021 gtttacccct ttggtctttc ttagtaatta tggtaagagt cagcattact ccagtgagaa
     1081 gtcagccttc ctcctttcca ttctggcctt tgttgatatg gtagccagac cttccatggg
     1141 acttgcagcc aacaccaagt ggatcagacc tcggatccag tacttctttg ctgcttctgt
     1201 ggttgccaat ggagtgtgcc atttgctagc acctttgtct acaagctaca tcgggttctg
     1261 tatctacgcg ggagtctttg gatttgcctg tggttggctc agctccgtat tatttgaaac
     1321 attgatggac cttgttggtc cccagaggtt ctccagtgct gtgggcttgg tgaccattgt
     1381 ggaatgctgt cctgtccttc tagggccacc acttttaggc cgccttaatg acatgtatgg
     1441 agactacaaa tacacgtact gggcttgtgg cgtgatcctc atcattgcag gtatctatct
     1501 cttcattggc atgggcatca attatcgact tgtggccaaa gaacagaaag cagaagagaa
     1561 gcagaaacag gaagaaggta aagaggacga caccagcact gatgttgatg agaaaccaaa
     1621 ggaattaaca aaagcaacag agtccccgca gcagaatagc tccggagacc ccgcagaaga
     1681 ggagagtcct gtctgaactg aagcatgaat agagcagtgt gtgacccaag acatctgaaa
     1741 ccattctgct ggcctctagt ctaccagtgg tgctcaatgc agacagtgga catttgtgtg
     1801 gaaaacctac caggtgttca ttggtggaat tttgttttgt tttgttttgt ttttgttttt
     1861 gttcactcct taccaatgcc agagtttaaa atttactatg ctttaggtag ggattggttg
     1921 gcaaaggata tgggaaagaa gtgtagggat ttgttttttt gtttttgttt tttttttaat
     1981 cttagctttt aacagtgtca tgaagattat aatatgtgcc ttaagtttta gttttagaac
     2041 tctttagaga gccttaactt ttaaaatcat tctgctgaat tcatctattt taaatgtttt
     2101 aaaaggaaaa ataacaacta acttgcttga ggtaactcta accttaatct tgttttgttg
     2161 ttgtttgtaa tgctttgtca gagattgtta ctggaacatt tatggataaa aatactagtt
     2221 aaaagttgga ggtttataaa atactgacta aagtattttt ctagcatcat agttgcctcg
     2281 agtaggtgcc tgctaggtat atatttaaga aatttaaagc atgaaattct ggaaacatct
     2341 tggctgttat agccacagtc tgtctgtctg tcatctgctg gggacttctc agatgcttac
     2401 tgcaagccta gtgctagaat gttgtcacta aattgctacc ttgctcctct tcagagacat
     2461 tgagtggtta caggtcattg ccatttttga aatctactgc aaaaaagtta gtattaaaat
     2521 ctacaaaact tttaacacag tctgatttaa tatagacagg tatttcaggc atctgctaaa
     2581 ttcagtttag ttttccaaaa ccctagttat ggtatgaaac tcttgtaatc tttgagtatg
     2641 tgtgggtttt ggggggcctg gtggttctga ggattgaacc cagggccaca agcatgctaa
     2701 gcacatgctg taccactgag ccacatccca ccagcaccct ggaatcctcc ttaaccccga
     2761 gacctttgct ctttgatttt tgatagttcc atttatatcc tagtttagag ctgtatgcga
     2821 gatatcagta tggactaaat gtgtgtgcta gttttgtttt ttacattgtt tttcagtatt
     2881 tgcaaaacca agagggccag agtttggccc cggggaagcc aataaagata aaatagggag
     2941 ggagtttgct gagttcactt gatttcagta agtacccacc tccctgccac atacaccaag
     3001 gcttaaagag aaacttatat catgcttaga attattggac atagccttac ctctacaaac
     3061 cttagctttc gtgacccttt tcacttacct gaaatataga aaatgggttc aatgtaagga
     3121 taggaggaag gatgggcagg attggaattg tagtattttt taaaaccaat atcttctaaa
     3181 tagagacaga aaagatatat gacactaaat tgtactcaat gcatctaaaa taccattgta
     3241 attgacgggg tgaaaatatc catttaaaac cttgtaagaa gccgactttt tccaaataaa
     3301 acatttattt tatttttaga aaaaaaaaaa aaaaagggcg gccgc
//