HDTenEightyP/NetNews huggingface dataset:
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Lentinus ponderosus ribosomal RNA (14LR6) large subunit, partial.
Date: 19 Aug 92 10:02:24 GMT
Distribution: bionet
Checksum: 22191     2

LOCUS       LENRRLSU      169 bp ss-rRNA            RNA       19-AUG-1992
DEFINITION  Lentinus ponderosus ribosomal RNA (14LR6) large subunit, partial.
ACCESSION   M98615
KEYWORDS    large subunit ribosomal RNA.
SOURCE      Lentinus ponderosus (library: Duke culture collection 585) cDNA to
            rRNA.
  ORGANISM  Lentinus ponderosus
            Eukaryota; Fungi; Eumycota; Basidiomycotina; Hymenomycetes;
            Agaricales; Tricholomataceae.
REFERENCE   1  (bases 1 to 169)
  AUTHORS   Hibbett,D.S. and Vilgalys,R.
  TITLE     Phylogenetic relationships of the basidiomycete genus Lentinus
            inferred from molecular and morphological characters
  JOURNAL   Unpublished (1992)
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     rRNA            <1..>169
                     /note="Approximately aligns to region between positions
                     845 to 1093in Saccharomyces cerevisiae large subunit
                     nuclear rRNA."
                     /product="large subunit ribosomal RNA"
                     /citation=[1]
BASE COUNT       54 a     28 c     37 g     50 t
ORIGIN
        1 aaatttgggt atagggaagc taatcgaacc atctagtagc tggttccgga agtttccctc
       61 aggatagaaa ctgtatcaga tttatgtggt aaaatgatta gaggccttgg ggttgaaaca
      121 accttaacct attctcaaac tttaaatatg taaacgagcc acttgattg
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Homo sapiens DNA-binding protein (ZNF) gene, partial cds.
Date: 21 Aug 92 10:11:47 GMT
Distribution: bionet
Checksum: 24552     2

LOCUS       HUMZNFD       264 bp ds-DNA             PRI       21-AUG-1992
DEFINITION  Homo sapiens DNA-binding protein (ZNF) gene, partial cds.
ACCESSION   M88360
KEYWORDS    DNA binding protein; zinc finger protein.
SOURCE      Homo sapiens Placenta DNA.
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 264)
  AUTHORS   Bray,P.L., Lichter,P., Thiesen,H.J., Ward,D.C. and Dawid,I.B.
  TITLE     Characterization and mapping of human genes encoding zinc finger
            proteins
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 88, 9563-9567 (1991)
  STANDARD  full automatic
REFERENCE   2  (bases 1 to 264)
  AUTHORS   Lichter,P., Bray,P., Ried,T., Dawid,I.B. and Ward,D.C.
  TITLE     Clustering of C2-H2 zinc finger motif sequences within telomeric
            and fragile site regions of human chromosomes
  JOURNAL   Genomics 13, 999-1007 (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     CDS             1..264
                     /standard_name="zinc finger protein"
                     /product="DNA binding protein"
                     /gene="ZNF"
                     /note="putative"
                     /codon_start=1
                     /translation="HQRVHTGERPYECDACGKAFSQSTHLTQHQRIHTGEKPYKCDAC
                     GRAFSDCSALIRHLRIHSGEKPYQCKVCPKAFAQSSSLIEHQRI"
                     /partial
BASE COUNT       62 a     82 c     76 g     44 t
ORIGIN
        1 caccagcggg tgcacacggg ggagcggccc tacgagtgtg acgcgtgtgg gaaagccttc
       61 agccagagca cgcacctgac tcaacaccag cgcatccaca ccggggagaa gccctacaag
      121 tgtgacgcgt gtggccgagc cttcagcgac tgctcagccc tgatccgaca tctgagaatc
      181 cactctggag agaagccata tcagtgtaag gtttgtccga aggcctttgc acagagctcc
      241 tccctcattg agcaccagag gatc
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Lenzites betulina ribosomal RNA (0LR0R) large subunit, partial.
Date: 19 Aug 92 10:02:33 GMT
Distribution: bionet
Checksum: 32285     2

LOCUS       LEQRRLS       223 bp ss-rRNA            RNA       19-AUG-1992
DEFINITION  Lenzites betulina ribosomal RNA (0LR0R) large subunit, partial.
ACCESSION   M98668
KEYWORDS    large subunit ribosomal RNA.
SOURCE      Lenzites betulina cDNA to rRNA.
  ORGANISM  Lenzites betulina
            Eukaryota; Fungi; Amastigomycota; Basidiomycetes.
REFERENCE   1  (bases 1 to 223)
  AUTHORS   Hibbett,D.S. and Vilgalys,R.
  TITLE     Phylogenetic relationships of the basidiomycete genus Lentinus
            inferred from molecular and morphological characters
  JOURNAL   Unpublished (1992)
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     rRNA            <1..>223
                     /note="Aligns to region between positions 70 to 306
                     inSaccharomyces cerevisiae large subunit nuclear rRNA."
                     /product="large subunit ribosomal RNA"
                     /citation=[1]
BASE COUNT       54 a     43 c     69 g     57 t
ORIGIN
        1 tagtaagcga gtgaagcgga aaagctcaaa tttaaaatct ggcggtcttt ggccgtccga
       61 gttgtagtct ggagaagtgc ttccgcgctg gaccgtgtac aagtctcttg gaacagagcg
      121 tcatagaggg tgagaatccc gtctttgaca cggactacca gtgctttgtg tagcgctctc
      181 aaagagtcga gttgtttggg aatgcagctc aaaatgggtg gtg
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human polymorphic microsatellite DNA.
Date: 19 Aug 92 10:01:00 GMT
Distribution: bionet
Checksum: 25809     2

LOCUS       HUM6PLYSAT    398 bp ds-DNA             PRI       19-AUG-1992
DEFINITION  Human polymorphic microsatellite DNA.
ACCESSION   M99146
KEYWORDS    microsatellite polymorphism.
SOURCE      Homo sapiens DNA.
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 398)
  AUTHORS   Bowcock,A.M.Ph.D.., Osborne-Lawrence,S.L., Barnes,R.I.,
            Chakravarti,A., Washington,S. and Dunn,C.
  TITLE     A linkage map of human chromosome 13q containing microsatellite
            polymorphisms
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     satellite       1..398
                     /note="map=13q; awaiting approval /map qualifier"
BASE COUNT       79 a     59 c     68 g    160 t     32 others
ORIGIN
        1 actccagcct ggacgacaga gcaagacgca gattccatcg tgctctctct ctctctctct
       61 ctctctctat atatatatat gtgtatatat atatatatac gtgtatatat atatacacgt
      121 atatatatat ggctagttta ttattatttt tttatctcta tatatatgtg tgtgtatgta
      181 tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtatata tggctcgctg tctctctcca
      241 tatatatata tatatatatg gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnggctagt
      301 ttattgactg ggtcacagtg ttgctttcca agggttattc ttactggctt atagtgtcct
      361 tcttttggat ctttgttcta ccttctgaaa cttatttt
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human chromosome 4 sequence-tagged site STS4-458
Date: 21 Aug 92 10:10:38 GMT
Distribution: bionet
Checksum: 43547     3

LOCUS       HUM4STS458    432 bp ds-DNA             PRI       21-AUG-1992
DEFINITION  Human chromosome 4 sequence-tagged site STS4-458
ACCESSION   L00874
KEYWORDS    STS; human chromosome 4; sequence tagged site.
SOURCE      Homo sapiens, clone p4-511 from a chromosome 4 plasmid library;
            vector pBluescript II KS + (Stratagene)
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 432)
  AUTHORS   Goold,R.D., diSibio,G., Dugaiczyk,A., Smith,K.A., Xu,H.,
            Magrane,G., Lang,D.B., Cox,D.R., Masters,S.B. and Myers,R.M.
  TITLE     Sequence-Tagged Sites for Human Chromosome 4
  JOURNAL   Unpublished (1992) see COMMENT for author address
  STANDARD  full automatic
COMMENT     PCR components: 25 ng of human genomic DNA, 10 pmol of each
            oligonucleotide, 200 micro-M dNTPs, 0.25 U Taq  polymerase (Cetus)
            in 10 micro-l of 50 mM KCl-20 mM  Tris-HCl, pH 8.3 (at room temp),
            2.5 mM MgCl-2.  Initial denaturation at 94degC for 1.5 min, then 30
            cycles of  94degC for 15 sec, 62degC for 23 sec, and 72degC for 30
            sec, followed by a final extension at 72degC for 3.5 min, using a
            Perkin-Elmer 9600 thermocycler. PCR-amplified product size 231 bp.
            Sequence submitted by:
            Human Genome Mapping Center
            Box 0925
            University of California San Francisco
            San Francisco, CA 94143-0925  USA
            Phone: (415) 502-1612   Fax: (415) 476-8391
            e-mail: hgmcprobes@cgl.ucsf.edu
FEATURES             Location/Qualifiers
     misc_feature    64..89
                     /label=Primer1
     misc_feature    complement(269..294)
                     /label=Primer2
BASE COUNT      140 a     72 c     69 g    143 t      8 others
ORIGIN
        1 gatctgaaaa catagaaaaa gcaaaatata aaactgtctg ataagtaaac ccaggcaaaa
       61 aaacgtatgt agacaattat gaagacattt taaattttat ttcattaaca acaattttat
      121 tttattttat ttatttttta ttatttntng ggngatggag tcttgntttc tcaccaggct
      181 ggagtgctgc agcacaatct tggctcactg caacctccgc ctcccaggtt caagcgattc
      241 ccctgcctca accaactagt ttaccaaaga tttacttacg tgacatgaac tttataaaat
      301 gggttaatta ctgtatattt natatgagtg ctcatttatt ganatcaatc aaaatacaaa
      361 tttcttaaga gatttctggc tggctacatc agattttatt atggtnaaca ggagatcagg
      421 ggtnatacat gt
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human chromosome 4 sequence-tagged site STS4-112
Date: 21 Aug 92 10:06:40 GMT
Distribution: bionet
Checksum: 22828     3

LOCUS       HUM4STS112    380 bp ds-DNA             PRI       21-AUG-1992
DEFINITION  Human chromosome 4 sequence-tagged site STS4-112
ACCESSION   M95256
KEYWORDS    STS; human chromosome 4; sequence tagged site.
SOURCE      Homo sapiens, clone C4-162 from Los Alamos National Laboratory
            chromosome 4 cosmid library, plate 4-1-2R, pos 12A; vector SuperCos
            1 (Stratagene)
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 380)
  AUTHORS   Goold,R.D., diSibio,G., Dugaiczyk,A., Smith,K.A., Xu,H.,
            Magrane,G., Lang,D.B., Cox,D.R., Masters,S.B. and Myers,R.M.
  TITLE     Sequence-Tagged Sites for Human Chromosome 4
  JOURNAL   Unpublished (1992) see COMMENT for author address
  STANDARD  full automatic
COMMENT     PCR components: 25 ng of human genomic DNA, 10 pmol of each
            oligonucleotide, 200 micro-M dNTPs, 0.25 U Taq  polymerase (Cetus)
            in 10 micro-l of 50 mM KCl-20 mM  Tris-HCl, pH 8.3 (at room temp),
            2.5 mM MgCl-2.  Initial denaturation at 94degC for 1.5 min, then 30
            cycles of  94degC for 15 sec, 62degC for 23 sec, and 72degC for 30
            sec, followed by a final extension at 72degC for 3.5 min, using a
            Perkin-Elmer 9600 thermocycler. PCR-amplified product size 292 bp.
            Sequence submitted by:
            Human Genome Mapping Center
            Box 0925
            University of California San Francisco
            San Francisco, CA 94143-0925  USA
            Phone: (415) 502-1612   Fax: (415) 476-8391
            e-mail: hgmcprobes@cgl.ucsf.edu
FEATURES             Location/Qualifiers
     misc_feature    17..41
                     /label=Primer1
     misc_feature    complement(286..308)
                     /label=Primer2
BASE COUNT      134 a     56 c     56 g    120 t     14 others
ORIGIN
        1 ttaaantaat agtnatgcct catataataa gcaatcgttt gctcatcctt caagtaatta
       61 agaatcagaa cagttagtag aggaagacaa actacttatt catggagttc ttaaatgtca
      121 agttacatga tgatatgtcc taagattgca ataaccctat gtgaaatttt acaaagcaat
      181 atataataat catttatatt aaaatatatt tangaaattt gnaggaccta agttaacatt
      241 ccagcaaagt tatgtgactt ttgnnaataa atactgttga accaacgcta gagaaatgac
      301 agtcaccgnt ctaagccatc agngtgggga tttnagantt tcttctcatt atnctaagat
      361 tngcctncca gaatttntat
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Gallus gallus Ig rearranged L-chain gene V-J-region, partial cds.
Date: 21 Aug 92 10:05:56 GMT
Distribution: bionet
Checksum: 31385     3

LOCUS       CHKIGLAZF     431 bp ds-DNA             VRT       21-AUG-1992
DEFINITION  Gallus gallus Ig rearranged L-chain gene V-J-region, partial cds.
ACCESSION   M96970
KEYWORDS    immunoglobulin light chain; joining region; processed gene;
            variable region.
SOURCE      Gallus gallus (individual_isolate REV-T (CSV), strain SC line
            (Hyline International, Dallas Center, Iowa), sub_species
            domesticus) (library: TB4(BF1) and TB3(TE11)) day 12 embryo bursa
            DNA.
  ORGANISM  Gallus gallus
            Eukaryota; Animalia; Chordata; Vertebrata; Aves; Neornithes;
            Neognathae; Galliformes; Phasianidae.
REFERENCE   1  (bases 1 to 431)
  AUTHORS   Benatar,T., Tkalee,L. and Ratcliffe,M.J.
  TITLE     Stochastic rearrangement of immunoglobulin variable-region genes in
            chicken B-cell development
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 89, 7615-7619 (1992)
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     intron          <1..105
                     /gene="IgL"
     CDS             106..>431
                     /note="V-J-region"
                     /product="immunoglobulin light chain"
                     /gene="IgL"
                     /codon_start=3
                     /translation="SLVQAALTQPSSVSANPGGTVKITCSGDSSYYGWYQQKAPGSAP
                     VTVIYDNTNRPSNIPSRFSGSKSGSTATLTITGVRADDNAVYYCASTDSSSTAGIFGA
                     GTTLTV"
     mat_peptide     123..431
                     /note="V-J-region"
                     /product="immunoglobulin light chain"
                     /gene="IgL"
                     /codon_start=1
     misc_recomb     396..398
                     /note="VJ junction"
                     /gene="IgL"
BASE COUNT       81 a    142 c    118 g     90 t
ORIGIN
        1 gggactgtgg gcacggggct ctgtcccact gctgcgcggg cagggctgtg cgtgcggggc
       61 cgtcactgat tgccgttttc tcccctctct cctctccctc tccaggttcc ctggtgcagg
      121 cagcgctgac tcagccgtcc tcggtgtcag cgaacccggg aggaaccgtc aagatcacct
      181 gctccgggga tagcagctac tatggctggt accagcagaa ggcacctggc agtgcccctg
      241 tcactgtgat ctatgacaac accaacagac cctcgaacat cccttcacga ttctccggtt
      301 ccaaatccgg ctccacagcc acattaacca tcactggggt ccgagccgac gacaatgctg
      361 tctattactg tgcgagtaca gacagcagca gtactgctgg tatatttggg gccgggacaa
      421 ccctgaccgt c
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Mouse gene IgE heavy chain C-region exons for membrane bound form
Date: 22 Aug 92 10:01:09 GMT
Distribution: bionet
Checksum: 23117     3

LOCUS       MUSIGEHC1     364 bp ds-DNA             ROD       22-AUG-1992
DEFINITION  Mouse gene IgE heavy chain C-region exons for membrane bound form
ACCESSION   X03624
KEYWORDS    constant region; epsilon-immunoglobulin;
            immunoglobulin heavy chain.
SOURCE      Mus musculus DNA.
  ORGANISM  Mus musculus
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Rodentia; Myomorpha; Muridae; Murinae.
REFERENCE   1  (bases 1 to 364)
  AUTHORS   Ishida,N., Ueda,S., Hayashida,H., Miyata,T. and Honjo,T.
  TITLE     The nucleotide sequence of the mouse immunoglobulin epsilon gene:
            comparison with the human epsilon gene sequence
  JOURNAL   EMBO J. 1, 1117-1123 (1982)
  STANDARD  full automatic
COMMENT     Exons 5 and 6 code for the membrane bound form of IgE and are about
            1.7 kb downstream of the CH4 domain coding exon 4. They are linked
            to exon 4 by alternative splicing. (see MMIGEHC)
            From EMBL    entry MMIGEHC1;  dated 10-JAN-1991.
FEATURES             Location/Qualifiers
     precursor_RNA   <1..>364
                     /note="IgE membrane bound form precursor"
     CDS             join(42..173,254..337)
                     /product="IgE membrane bound form"
                     /codon_start=1
                     /translation="LDLQDLCIEEVEGEELEELWTSICVFITLFLLSVSYGATVTVLK
                     VKWVLSTPMQDTPQTFQDYANILQTRA"
                     /partial
     mRNA            40..173
                     /note=" (membrane)"
     intron          174..253
                     /note="intron V"
     mRNA            254..>364
BASE COUNT       78 a    110 c    104 g     72 t
ORIGIN
        1 gtctggggaa gctaactggc tggtcccacc ccatcccaga gctagacctc caggacctat
       61 gtattgaaga ggtggagggc gaggagctgg aagagctgtg gaccagtatt tgtgtcttca
      121 tcaccctgtt cctgctcagt gtgagctatg gggccactgt caccgtcctc aaggtgggat
      181 cctgtacctc agcgggtggg tccgggaggg ccaggcaagc cgcagagcat cctcacatac
      241 acctttcccc taggtgaagt gggtcctctc cacaccgatg caggatacac cccagacctt
      301 ccaagactat gccaacatcc tccagaccag ggcataggtg cgatgccagc accatgcagg
      361 cctg
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human immunodeficiency virus type 2 (FOENVA33) envelope protein
Date: 18 Aug 92 10:01:33 GMT
Distribution: bionet
Checksum: 45422     2

LOCUS       HIVENVA33     453 bp ss-RNA             VRL       18-AUG-1992
DEFINITION  Human immunodeficiency virus type 2 (FOENVA33) envelope protein
            fragment.
ACCESSION   M87087
KEYWORDS    envelope protein.
SOURCE      Human immunodeficiency virus type 2 uncultured blood mononuclear
            cells (11/89 sample) RNA.
  ORGANISM  Human immunodeficiency virus type 2
            Viridae; ss-RNA enveloped viruses; Positive strand RNA virus;
            Retroviridae; Lentivirinae.
REFERENCE   1  (bases 1 to 453)
  AUTHORS   Gao,F., Yue,L., White,A.T., Pappas,P.G., Barchue,J., Hanson,A.P.,
            Greene,B.M., Sharp,P.M., Shaw,G.M. and Hahn,B.H.
  TITLE     Human infection by genetically-diverse SIVsm-related HIV-2 in West
            Africa
  JOURNAL   Nature (1992) In press
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     misc_feature    1..453
                     /note="PCR derived HIV-2 envelope fragment"
                     /gene="env"
                     /note="putative"
                     /citation=[1]
BASE COUNT      160 a     78 c    105 g    110 t
ORIGIN
        1 gacgtggtca agagacaaca agaattgttg cgactgaccg tctgggggac taaaaacctc
       61 cagactagag tcactgctat cgagaagtac ctaaaggacc aggcgcagct aaattcatgg
      121 ggatgcgcgt tcaggcaggt ctgccacacc actgtagaat ggccaaatag tactctcaca
      181 cctgactaga acaatatgac ttggcaggag tgggagagac aggttgattt cctagaggca
      241 aatataacac aattattaga ggaagcacaa attcaacaag aaaagaacat gtatgagtta
      301 caaaaactaa ataactggga tatatttggc aactggtctg accttacttc ttggattaag
      361 tatatacagt atggagtctt catagtagta ggtataatac tgttaagaat aataatctat
      421 gtagtacaaa tgttagctaa gttaagtaag gga
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Mouse T cell receptor rearranged alpha-chain variable region,
Date: 15 Aug 92 10:02:34 GMT
Distribution: bionet
Checksum: 18377     2

LOCUS       MUSTCR8B      496 bp ss-mRNA            ROD       15-AUG-1992
DEFINITION  Mouse T cell receptor rearranged alpha-chain variable region,
            N_region, joining region, and constant region mRNA.
ACCESSION   M98568
KEYWORDS    T-cell receptor; alpha-subunit receptor; constant region;
            joining region; variable region.
SOURCE      Mus musculus (strain BALB/cKh) adult spleen cDNA to mRNA.
  ORGANISM  Mus musculus
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Rodentia; Myomorpha; Muridae; Murinae.
REFERENCE   1  (bases 1 to 496)
  AUTHORS   Solheim,J.C., Martinko,J.M., Alexander-Miller,M. and Connolly,J.M.
  TITLE     Biased T Cell Receptor Usage by Ld-restricted Tum-Peptide-specific
            Cytotoxic T Lymphocyte Clones
  JOURNAL   Unpublished (1992)
  STANDARD  full staff_entry
FEATURES             Location/Qualifiers
     sig_peptide     1..60
                     /gene="TCRA"
                     /codon_start=1
BASE COUNT      100 a    139 c    135 g    122 t
ORIGIN      Chromosome 14
        1 atgctcctgg cactcctccc agtgctgggg atacactttg tcctgagaga tgcccaagct
       61 cagtcagtga cacagcccga tgctcgcgtc actgtctctg aaggagcctc tctgcagctg
      121 agatgcaagt attcctactc tgggacgcct tatctgttct ggtatgtcca gtacccgcgg
      181 caggggctgc agctgctcct caagtactat tccggagacc cagtggttca aggagtgaat
      241 ggctttgagg ctgagttcag caagagtaac tcttccttcc acctgcggaa agcctccgtg
      301 cactggagcg actcggctgt gtacttctgt gctctgagcg gggggagata gaggttcagc
      361 cttagggagg ctgcattttg gagctgggac tcagctgatt gtcatacctg acatccagaa
      421 cccagaacct gctgtgtacc agttaaaaga tcctcggtct caggacagca ccctctgcct
      481 gttcaccgac tttgac
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human chromosome 4 sequence-tagged site STS4-367
Date: 21 Aug 92 10:09:32 GMT
Distribution: bionet
Checksum: 01147     3

LOCUS       HUM4STS367    322 bp ds-DNA             PRI       21-AUG-1992
DEFINITION  Human chromosome 4 sequence-tagged site STS4-367
ACCESSION   L00822
KEYWORDS    STS; human chromosome 4; sequence tagged site.
SOURCE      Homo sapiens, clone p4-285 from a chromosome 4 plasmid library;
            vector pBluescript II KS + (Stratagene)
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 322)
  AUTHORS   Goold,R.D., diSibio,G., Dugaiczyk,A., Smith,K.A., Xu,H.,
            Magrane,G., Lang,D.B., Cox,D.R., Masters,S.B. and Myers,R.M.
  TITLE     Sequence-Tagged Sites for Human Chromosome 4
  JOURNAL   Unpublished (1992) see COMMENT for author address
  STANDARD  full automatic
COMMENT     PCR components: 25 ng of human genomic DNA, 10 pmol of each
            oligonucleotide, 200 micro-M dNTPs, 0.25 U Taq  polymerase (Cetus)
            in 10 micro-l of 50 mM KCl-20 mM  Tris-HCl, pH 8.3 (at room temp),
            2.5 mM MgCl-2.  Initial denaturation at 94degC for 1.5 min, then 30
            cycles of  94degC for 15 sec, 62degC for 23 sec, and 72degC for 30
            sec, followed by a final extension at 72degC for 3.5 min, using a
            Perkin-Elmer 9600 thermocycler. PCR-amplified product size 202 bp.
            STS sequence contains a simple sequence tandem repeat (CA).
            Sequence submitted by:
            Human Genome Mapping Center
            Box 0925
            University of California San Francisco
            San Francisco, CA 94143-0925  USA
            Phone: (415) 502-1612   Fax: (415) 476-8391
            e-mail: hgmcprobes@cgl.ucsf.edu
FEATURES             Location/Qualifiers
     misc_feature    14..37
                     /label=Primer1
     misc_feature    complement(191..215)
                     /label=Primer2
BASE COUNT      120 a     62 c     44 g     94 t      2 others
ORIGIN
        1 gatcttagaa aggcagagtc ataacaggaa gaaaccaaag accttggtgt attagtcagg
       61 gttttccaga gaagccaaac caataacata tatatatata catacatata tatatacaca
      121 cacacacaca cacacacaca cacacacatn tgtatgtaca ttattaattc aaagagaatt
      181 cttttacttt cttggaaatc actctatcct taaagtatgc atatatatta ggagtattct
      241 gctgattatt aatcatagct aacatccagt taacaggctg tagntcattt aaacaaacat
      301 gtattcacat caggtgtgtc ct
//