From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Serratia marcescens C protein, endonuclease, and methylase genes,
Date: 14 Aug 92 10:01:23 GMT
Distribution: bionet
Checksum: 57676     5

LOCUS       SMARME       1945 bp ds-DNA             BCT       14-AUG-1992
DEFINITION  Serratia marcescens C protein, endonuclease, and methylase genes,
            complete cds.
ACCESSION   M98769
KEYWORDS    C protein; endonuclease; methylase.
SOURCE      Serratia marcescens DNA.
  ORGANISM  Serratia marcescens
            Prokaryotae; Gram-negative facultatively anaerobic rods;
            Enterobacteriaceae.
REFERENCE   1  (bases 1 to 1945)
  AUTHORS   Dunbar,J.C. and Withers,B.
  TITLE     Characterization of the SmaI restriction and modification enzymes
  JOURNAL   Unpublished (1992)
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     CDS             38..292
                     /product="C protein"
                     /note="putative"
                     /codon_start=1
                     /translation="MPDADICFMDIKEILAENVRSYRNINNLSQEQLAEISGLHRTYI
                     GSVERKERNVTLSTLIILRKALNTSVPKLLTRQGLKNEQG"
     CDS             279..1022
                     /product="endonuclease"
                     /evidence=EXPERIMENTAL
                     /codon_start=1
                     /translation="MSRDDQLFTLWGKLNDRQKDNFLKWMKAFDVEKTYQKTSGDIFN
                     DDFFDIFGDRLITHHFSSTQALTKTLFEHAFNDSLNESGVISSLAESRTNPGHDITID
                     SIKVALKTEAAKNISKSYIHVSKWMELGKGEWILELLLERFLEHLENYERIFTLRYFK
                     ISEYKFSYQLVEIPKSLLLEAKNAKLEIMSGSKQSPKPGYGYVLDENENKKFSLYFDG
                     GAERKLQIKHLNLEHCIVHGVWDFILPPP"
     CDS             complement(1008..1886)
                     /product="methylase"
                     /evidence=EXPERIMENTAL
                     /codon_start=1
                     /translation="MKKHSNNLDLFDQTEECLESNLRSCKIIVGDAREAVQGLDSEIF
                     DCVVTSPPYWGLRDYGNGGQIGAEDNINDYIKDLVDLFRDVRRTLKDDGTLWLNIGDS
                     YTSGGRTWRDKDDKNKGRAMSYRPPTPEGLKPKDLIGVPWRLAFALQNDGWYLRTDII
                     WNKPNCQPESVRDRPTRSHEYIFLLSKGKKYYYDWESIKEPASDPKMDKKNRRTVWNI
                     NTEPYPGSHFAVFPRAMARLCVLAGSRPGGKVLDPFFGSGTTGVVCQELDRECVGIEL
                     NEEYASLAKERILRRR"
BASE COUNT      624 a    367 c    351 g    603 t
ORIGIN
        1 tcattataaa ttccctttaa tttaaagggg atgacatgtg cctgacgctg acatctgttt
       61 catggatatt aaagaaatcc ttgctgaaaa tgtaaggagc tacaggaata tcaataattt
      121 atcgcaagaa cagttagcgg aaatatccgg tttgcacaga acttacatag gttctgttga
      181 aagaaaagaa agaaatgtaa ctctaagtac tcttattatt ttacgtaaag cattgaatac
      241 ttccgtacca aaactcctaa caaggcaggg tttaaaaaat gagcagggat gaccaactct
      301 ttacactttg gggaaagctt aacgatcgtc agaaggataa ttttctaaaa tggatgaaag
      361 cttttgatgt agagaaaact taccaaaaaa caagtgggga tattttcaat gatgattttt
      421 tcgatatatt tggtgataga ttaattactc atcatttcag tagcacgcaa gctttaacaa
      481 aaactttatt cgaacatgct tttaatgact ccttaaatga atctggagtt atatcctctc
      541 ttgcggaaag tagaacaaac cctgggcatg acataacaat cgatagcata aaggttgctt
      601 taaaaacaga agcagctaaa aatattagca aatcatatat tcatgtaagt aagtggatgg
      661 agttaggcaa gggggagtgg attctagaat tattattaga acggttttta gagcatctag
      721 agaattatga acgtattttc acactcagat attttaaaat atccgagtat aaatttagct
      781 accagcttgt agaaataccc aagagtcttt tgttggaagc aaaaaatgcg aaattagaaa
      841 taatgtcggg aagcaaacaa agccctaagc ccggctatgg atatgtgtta gatgaaaatg
      901 aaaataagaa gttttctcta tactttgatg gtggtgccga gagaaaactt caaataaaac
      961 atttaaattt agaacattgc attgttcatg gagtttggga ttttattcta ccgccgcctt
     1021 aatattctct ctttggcaag agaagcatat tcttcattga gctcgatacc gacacactct
     1081 ctatcgagtt cttggcaaac aacccctgtg gttcccgatc caaaaaacgg atcaagaact
     1141 tttcctccgg gacgtgagcc agccaacaca cataacctag ccatggcacg tgggaaaaca
     1201 gcgaagtgac ttcctggata cggttctgtg ttaatattcc aaacagttcg ccggttcttc
     1261 ttatccattt taggatcaga tgccggttct ttaattgatt cccaatcata gtaatatttt
     1321 ttccctttag ataagagaaa tatatattca tgtgatctag ttggtctatc tctgacgctt
     1381 tcgggttgac agttcggttt gttccatatg atatcagtcc ttaaatacca accatcattt
     1441 tgaagtgcga atgccagccg ccatggaaca ccgatcaaat cttttggttt taaaccttcc
     1501 ggcgtaggcg gtctatagct cattgctcgc cctttgtttt tatcatcttt atcacgccaa
     1561 gttcttcctc cagatgtata gctgtcacct atattaagcc atagcgttcc atcatcttta
     1621 agagttcttc ttacatctct aaatagatca actaaatctt ttatgtaatc atttatgtta
     1681 tcttcagcgc ctatttgacc gccattaccg taatctctta acccccaata aggtggggat
     1741 gtaacaacac agtcaaatat ttcagaatct aacccctgaa ctgcttccct agcatcgcct
     1801 acaataattt tgcaagaacg caggttgctc tccaaacact cttctgtttg atcgaataag
     1861 tctaaattgt tactgtgttt tttcatagaa ctcaaagaaa caaagaaata atgctactta
     1921 tggtattcat ttgagagcgt aaaaa
//