From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Giardia intestinalis surface protein 11 (tsp11) gene, complete cds.
Date: 12 Jan 93 05:46:21 GMT
Distribution: bionet
Checksum: 61013 7

LOCUS       GIAMSP       3356 bp ds-DNA             INV       11-JAN-1993
DEFINITION  Giardia intestinalis surface protein 11 (tsp11) gene, complete cds.
ACCESSION   M95814
KEYWORDS    major surface antigen; major surface protein; surface protein 11.
SOURCE      Giardia lamblia (strain Adelaide-1) vegetative trophozoite DNA.
  ORGANISM  Giardia lamblia
            Eukaryota; Animalia; Protozoa; Sarcomastigophora; Mastigophora;
            Zoomastigophora; Diplomonadida; Diplomonadina; Hexamitidae.
REFERENCE   1  (bases 1 to 3356)
  AUTHORS   Ey,P.L., Khanna,K., Manning,P.A. and Mayrhofer,G.
  TITLE     A gene encoding a Mr 69-kDa major surface protein of Giardia
            intestinalis trophozoites
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     5'UTR           1..1260
     mRNA            1256..>3278
                     /gene="tsp11"
                     /evidence="EXPERIMENTAL"
     sig_peptide     1261..1311
                     /gene="tsp11"
                     /codon_start=1
     mat_peptide     1312..3261
                     /product="surface protein 11"
                     /gene="tsp11"
                     /codon_start=1
     polyA_signal    3273..3278
     CDS             1261..3264
                     /standard_name="major surface protein 11"
                     /gene="tsp11"
                     /product="surface protein 11"
                     /codon_start=1
                     /translation="MLLAIFYFVISTLTAKCTTQTQTCEAEKCEMVGETEICTRCQTG
                     KVPIDGKCVDATANANCKNASGDGDANQVCGKMSSVPGNTLCTTVSPDGVCSVAANEY
                     FVPPNADATHDSVVSCSEETPIHLANNKQYIGVAGCATCSAPKAPGEDNTPKAATCTK
                     CAAGFLHTPSEGLSSCEETCPEGYFGHTATAESKKTCKSCTGGSSEAPNVKGIGDCLK
                     CMYNEASGNTLTCEKCSAQKKPSLDKTSCNDCTGQNCAFCSSSGGDCEGCDSGFILDG
                     QNCVKSDCKTENCKACTNPKAANEVCTECISTHHLTPTSQCVQYCQALGNYYAGTNAD
                     NKKACKECTVANCKTCNDQGQCQTCNDGFYKNGDACSPCHESCKTCSAGTASDCTECP
                     TGKALKYGNDGTKGTCGEGCTTGQGSGACKTCGLTIDGASYCSECDTQNEYPQNGICT
                     STTARTVATCKNSNVANGICSSCTNGFLRMNGGCYETTKFPGKSVCEGANADADTCKA
                     PVPGYKVEAGKLVMCSKGCDTCSDATTCTKCGDGYTKIENSQTCTKCDSSCETCTGAA
                     TTCKVCATGYYKTALGESTCTSCENDSNGVIGVKGCLNCAPPSSSTGSVLCYLMKGEN
                     TGGSVNKSGLSTGAIAGISVAAVVVVAGLVGFLCWWFICRGKA"
BASE COUNT      926 a    773 c    898 g    759 t
ORIGIN      
        1 gatcctacga ttccaagagc tgacccctcc cgagagcatt agcaagcgct tatagtgacg
       61 cggagtagag gctgaccaat gcaagtcgac gcacactgtc gatcatagag gggagcgcca
      121 agccgtagca gtaggacttt tactgcgtgg tttgctggct cgcaacgcag gagataacgt
      181 gtctgtatcg accagtttat ctgttcaaga ttggggacag cttagacaga ggttgcagac
      241 aggaactggt tagcttgctt gtcagaaact ggaggcctga tttctccgcg tcctacatgt
      301 gggttcaaag aatggtggat ccggagacag acacgcaaat gataaaaaat ggcaactcat
      361 tacaacctcc ccgggcgtct gctcccacct tttgcaagca tttgtatgct agaggagctt
      421 tagtgcagag aattctgatg acgcaggcag cttctcggtg gttagcatcc acgtagagag
      481 tcccgtctct tgagacctgc agattcagta cgcgcttagg agttacgggt atggatactc
      541 taagacagac gtgtctatgg atgcagctgg gtaccagtac ttgtccctgc attaaccgtt
      601 gccatgattg ggttgtgcag cgtgtcctgt ccgctgaccc gagatagcac gtttggacaa
      661 tgccgctttc tatgcgggcc tggaagaatt aagctttttg aagtgttgcc agtcaaccca
      721 tcgcacaatg agaggctgta ccaatagcct agcgaagatg cagaaacaag ccgtgcacgt
      781 tccagaccgg agtgcccagc aagccagtct gttgctagca tacacctcct acaggcttct
      841 cggttgccac aatctccttt cggaatcctg caaagaaccg tttctgggct tatcccggat
      901 gccgggcttt gagtatgacc cgcgcaggac cagcagcagg cataaagcgt agagaatacc
      961 caggatgcca gctaattcca cagagtaagg gaggtaggca caaaggacag cgctgttgtc
     1021 tatttctgaa gtgtctgttc ccgctgcaca gaggggtcgc agagtctgta ttcggcggag
     1081 aaaggggcta cgcaagggac gcgtctaacg cttgacgaag tatatttccc aacccagagc
     1141 cacataaaag ggttccctta gactagtcat ccgtagatac aacaaccaga atcttaggct
     1201 tgggtgcttg agattattaa gaagattctt acccgtataa gtgagctgag aaggatttta
     1261 atgttgcttg caattttcta ctttgtgata agcactctta cagcaaagtg tactacgcag
     1321 acacaaactt gtgaagctga gaagtgcgaa atggttggtg aaacagaaat ctgtacacgg
     1381 tgtcaaacag gaaaagttcc aattgatgga aagtgcgtag atgctactgc taatgcaaac
     1441 tgcaaaaacg ctagcggtga tggtgatgca aaccaagtct gcgggaaaat gtcatcggta
     1501 ccgggaaata ctctgtgcac aactgtgtca ccagatggag tatgctcagt agctgcgaat
     1561 gaatactttg ttccaccaaa tgcggatgct actcatgatt cagtcgtatc atgttctgag
     1621 gaaaccccaa tacatcttgc taataacaag cagtacatag gcgtagcggg atgtgctact
     1681 tgcagtgctc ctaaggctcc tggtgaagac aacaccccaa aggctgctac gtgtactaag
     1741 tgtgcagcag gatttctaca tactccatct gaaggactga gttcatgcga agaaacatgc
     1801 ccagagggat acttcgggca cacagctact gctgaaagca agaagacttg caaatcatgc
     1861 acaggcggat ctagcgaagc accgaatgta aagggtattg gagactgtct gaagtgcatg
     1921 tacaatgagg ctagcggcaa tacccttacg tgcgaaaaat gcagtgcaca aaagaagcca
     1981 agtctagata aaacaagttg taatgattgt acaggtcaaa actgcgcttt ctgtagttct
     2041 agtgggggag actgtgaggg atgtgactct ggtttcatct tggatggaca aaactgtgtt
     2101 aagagcgact gcaagactga gaactgcaag gcgtgcacca atccgaaggc agcaaacgag
     2161 gtctgcacag agtgcatctc cactcaccat ctcacgccga caagtcaatg tgtgcagtac
     2221 tgtcaagcac ttggaaacta ctatgctggc actaatgctg acaacaaaaa ggcatgcaag
     2281 gagtgcactg tggctaactg caaaacttgc aatgatcagg ggcagtgtca aacatgcaac
     2341 gacggattct ataaaaacgg cgacgcatgc tctccgtgcc acgaaagctg caagacatgc
     2401 agcgcaggca ctgccagcga ctgcaccgag tgtcccactg gaaaagcact taagtatgga
     2461 aacgacggta ctaagggcac gtgtggagaa ggctgcacaa caggccaagg cagcggtgcc
     2521 tgcaaaacgt gtgggctcac tatcgatggt gctagctact gctctgagtg tgatacacaa
     2581 aatgaatatc ctcaaaatgg gatctgtact tcaacgaccg ctcgtactgt ggctacttgt
     2641 aagaatagca acgttgccaa tggcatatgt agctcatgta ccaatggatt tcttcgtatg
     2701 aacggaggct gctatgagac gaccaagttt ccgggaaaga gcgtctgcga aggtgcaaac
     2761 gcagacgcag acacgtgcaa agcacctgtt ccaggttaca aagtagaggc aggaaagctt
     2821 gtgatgtgct ctaagggctg tgacacgtgc agcgacgcaa ctacttgtac aaaatgtgga
     2881 gatggataca caaaaattga aaacagccaa acatgcacaa agtgtgattc cagctgtgaa
     2941 acatgtactg gagcagctac aacatgtaag gtgtgtgcta caggatacta caagaccgca
     3001 ttgggagaaa gtacgtgcac atcctgcgaa aatgatagca acggagtcat cggtgtcaag
     3061 ggctgcttaa actgcgctcc tccatcgtct agtactggct ccgttctctg ctacctcatg
     3121 aaaggtgaga atactggtgg aagcgtcaac aagagcggcc tttccactgg cgccatcgcg
     3181 gggatctccg tcgcagccgt cgtcgtcgtg gccggccttg tgggcttcct ctgctggtgg
     3241 ttcatctgcc gtggaaaggc gtagacttag atagtaaacg cgtcactgta tatggcttat
     3301 tacgctgtgc tgctggggct atctgagagc ggtccagaga tcatgcgagt gggccc
//