HDTenEightyP/NetNews huggingface dataset:
From: k_garson@uk.ac.icrf
Newsgroups: biosci.methods-and-reagents
Subject: SDS in Flakes
Date: 23 Jul 92 10:40:59 GMT
Organization: Imperial Cancer Research Fund, London, UK

Hello,

I wonder if anyone could give me the name of a company that sells
SDS in flakes. I used to use such a product in Canada, but cannot
find a supplier in the UK.  Weighing out the SDS was much easier as
one didn't have to contend with SDS powder spilling into the air.
I will appreciate all responses.

Ken Garson
ICRF
London, UK
k_garson@ICRF.AC.UK
Newsgroups: bionet.molbio.methds-reagnts
From: pcj1@cunixf.cc.columbia.edu (Pierre Jelenc)
Subject: Re: SS Phagemid gradients, KI or CsCl?
Reply-To: pcj1@cunixf.cc.columbia.edu (Pierre Jelenc)
Organization: Columbia University
Date: Thu, 23 Jul 1992 18:40:12 GMT

In article <1992Jul23.025622.9263@menudo.uh.edu> bchs1b@Elroy.UH.EDU writes:
>In article <1992Jul21.154049.25431@usenet.ins.cwru.edu>, djt2@po.CWRU.Edu (Dennis J. Templeton) writes:
>>
>>We're trying to purify a mg or so of phagemid DNA, and need it to be free
>>from contaminating double stranded DNA, such as might come from broken
>>cells.  The protocol (sketchy) we're following suggests using EtBr/KI
>>gradients for this, and collecting the lower band (SS) and discarding the
>>upper band.  Our first go at this was a bust, as we lost almost all of the

>
>Instead of running gradients, why don't you treat the lysate with DNAse,
>which will not get into the phage heads, then PEG ppt your phage (twice
>if you want to be paranoid) phenol extract and etoh ppt. Should be
>free of ds DNA.
>
It does not quite work. In our hands, there is always some ds DNA left.

Pierre


Pierre Jelenc                        pcj1@cunixf.cc.columbia.edu 
                                    Columbia University, New York
From: suter@de.dbp.MPG.MPIZ-KOELN.VAX
Newsgroups: bionet.molbio.methds-reagnts
Subject: antisense
Date: 27 Jul 92 13:00:53 GMT
Distribution: bionet

dear netters,

something has been puzzling me for some time. 
a lot of people are using anti-sense RNA to inactivate transcripts. Does 
this mean that the endogenous sense transcript is indeed destroyed 
in the target cells ? does it completely disappear ? what is 
the proposed mechanism for breakdown of a double stranded
RNA molecule (are there enzymes that recognize these moieties) ?
 
cheers ! clemens
----------------------------------> cut here <----------------------------------
Clemens Suter-Crazzolara, PhD
Max-Planck-Institut fuer Zuechtungsforschung
Abteilung Genetische Grundlagen der Zuechtungsforschung
Carl-von-Linne Weg 10
5000 Koeln 30
Tel. xx49-221-5062.221           fax. xx49-221-5062.213
-------------------------------> end of message <-------------------------------
From: bchs1b@Elroy.UH.EDU (Michael Benedik)
Newsgroups: bionet.molbio.methds-reagnts
Subject: Re: Ecoli strains
Date: 23 Jul 92 20:25:52 GMT
Reply-To: bchs1b@Elroy.UH.EDU
Distribution: bionet
Organization: University of Houston

In article <23013.9207231111@crc.ac.uk>, sbhattac@mrc-crc.ac.uk (Dr. Shoumo Bhattacharya B1 061) writes:
>Can anyone tell me of a source where I can find E.coli strains and 
>their genotypes?  Also are E. coli GM48 methylation defective (dam/dcm)? Many thanks  
>Shoumo Bhattacharya

Since there are hundreds of thousands (at least) different E. coli
strains around, there is no such thing as a master list. ASsuming you are
interested mostly in cloning strains, the New England Biolabs catalog
has a comprehensive list of standard strains and genotypes. GM48 is
supposed to be dam- dcm-


	---------------------------------------------------
	 Michael Benedik
	 Department of Biochemical and Biophysical Sciences
	 University of Houston
	 INTERNET: Benedik@UH.EDU	BITNET: Benedik@UHOU
From: bioysl@lure.latrobe.edu.au
Newsgroups: bionet.molbio.methds-reagnts
Subject: SEQUECING PCR PRODUCT
Date: 31 Jul 92 00:31:33 GMT
Organization: VAX Cluster, Computer Centre, La Trobe University

HI! FRIENDS!

   COULD ANYONE TELL ME ANY METHODS WHICH I CAN DIRECTLY SEQUECE MY PCR PRODUCT
WITHOUT CLONING IT FIRST?

   THANKS IN ADVANCE.

YONHSHU LIU
BIOCHEM. DEPT
LA TROBE UNI. 
AUSTRALIA
From: camdna@ubvmsb.cc.buffalo.edu (Darren Natale)
Newsgroups: bionet.molbio.methds-reagnts
Subject: Re: Smiles on Southerns
Date: 30 Jul 92 17:39:00 GMT
Distribution: bionet
Organization: University at Buffalo
News-Software: VAX/VMS VNEWS 1.41

In article <1992Jul30.124208.22038@gserv1.dl.ac.uk>, ray@uk.ac.le ("Dr R. Dalgleish") writes...
>If you run a large amount of DNA in a lane of a gel containing ethidium bromide
>the DNA tends to bind the dye. If the ethidium bromide is limiting then there
>may not be sufficient to saturate the DNA. At the _edges_ of the lane there
>will be more ethidium bromide available (it will be able to diffuse in from the
>spacer agarose between lanes) and so the DNA at the edges will tend to have
>more ethidium bromide bound. The efect of this will be to retard the DNA at the
>lane edges, hence smiling.
> 

Empirical observation: smiling within a lane occurs whenever DNA is overloaded.
I therefore disagree with the above hypothesis since I _never_ use EtBr in
my gels. Besides, the DNA in the middle of the lane (as well as the edge) is 
continually being "replenished" with EtBr upon electrophoresis, since EtBr and
DNA are oppositely charged.

DN
From: MRM@EPAVAX.RTPNC.EPA.GOV
Newsgroups: bionet.molbio.methds-reagnts
Subject: Glucocorticoid receptor and Ab
Date: 23 Jul 92 15:22:56 GMT
Distribution: bionet

Does anyone have:

1-Isolated purified human glucocorticoid receptor protein?

2-An antibody to the above suitable for use in gel shift assays?

Can you part with a small quantity of these?

Thanks,

Marc J. Mass, Ph.D.
MRM@epavax.rtpnc.epa.gov
Newsgroups: bionet.molbio.methds-reagnts
From: MREEBEN@BIOMED.UIO.NO (Mati Reeben)
Subject: two first PCR cycles with long times?
Organization: The Norwegian EMBnet Node
Date: Fri, 24 Jul 1992 22:08:31 GMT
X-News-Reader: VMS NEWS 1.22


Accidentally in a recent research article in Science from 3 July 1992 Vol. 257
p.55 I came over following PCR conditions. 

...All PCR were performed on single stranded cDNA's generated from
poly(A)+-enriched RNA from different brain regions.
The PCR conditions with nonredundant oligonucleotides consisted of two cycles
at 95 C (5 minutes), 62 to 68 C (2 minutes), and 72 C (10 minutes) followed by
25 cycles of 95 C (1 min), 62 to 68 C (2 minutes), and 72 C (2 minutes).

I would be grateful, if somebody can explain me the sense of these first two
cycles. OK, I understand initial more thorough denaturation, but why second
long denaturation ? And why two 10 min extensions in the beginning ? Is initial
template considered to be worse for elongation ? And how much this trick can
improve PCR ?

Besides any hints I would really appreciate  some references on the theme.
Thank you in advance.

Regards,

Mati
From: rybicki@uctvax.uct.ac.za
Newsgroups: bionet.molbio.methds-reagnts
Subject: dsDNA, denaturation, sequencing
Date: 21 Jul 92 11:42:55 GMT
Organization: University of Cape Town

Completely fool-proof dsDNA denaturing for sequencing:

Ku-chuan Hsiao (1991) NAR 19:2787.

Simply dissolve minprep DNA in 25-50ul TE; take 5ul, add 1 ul seq primer
(10ng/ul); add 1ul 1M NaOH, incub 37degC 10min; add 1ul 1M HCl (incub 10 min if
like); add 2ul Sequenase buffer; incub 37DegC 5 min if like, then follow
Sequenase protocol....  No ppte and desalt and resuspension, cuts max time off
many sequencing reactions, simple enough that honours students take to it like 
canards to HOH. 

Also strongly recommended by the famous Di "deoxy" James, our sequencer par
excellence.
 _________________________________________________________________________
|                                 |                                       |
|  Ed Rybicki                     |    Now you've got the hang of it      |
|  Dept Microbiology              | There's nothing you can't do with it  |
|  University of Cape Town        |         If you're very into it        |
|  PB Rondebosch 7700             |         You can't go wrong....        |
|  South Africa                   |                                       |
|  fax 27 21 650 4023             |              - Mad John               |
|  ed@micro.uct.ac.za             | (Ogden's Nut-Gone Flake, Small Faces) |
|_________________________________|_______________________________________|
Newsgroups: bionet.molbio.methds-reagnts
From: kim@m44.unm.edu
Subject: Re: SDS in Flakes
Date: Thu, 23 Jul 92 17:21:08 GMT
Organization: UNM Cancer Center, Albuquerque, NM
Distribution: bionet
Reply-To: kim@m44.unm.edu

In article <1992Jul23.093815.721@gserv1.dl.ac.uk>, K_GARSON@UK.AC.ICRF writes:
>
>I wonder if anyone could give me the name of a company that sells
>SDS in flakes. I used to use such a product in Canada, but cannot
>find a supplier in the UK.  Weighing out the SDS was much easier as
>one didn't have to contend with SDS powder spilling into the air.
>I will appreciate all responses.


I just take on faith the mass of SDS stated on the bottle of powder and add
water for a 10% stock solution.  This obviates the need to weigh out powder.

Lots of people will give you this answer.

Daniel Kim
From: hsieh@mcclb0.med.nyu.edu (Chung-Ming Hsieh)
Newsgroups: bionet.molbio.methds-reagnts
Subject: RSV LTR
Date: 23 Jul 92 04:33:47 GMT
Organization: NYU Medical Center, New York, NY 10016, USA

I would like to know if anyone out there has experience with the
enhancer/promotor activity of the ritrovirus RSV LTR especially
in HeLa cells?  What is the known factors that regulate its activity?

Chung-Ming Hsieh
NYU Medical Center
Newsgroups: bionet.molbio.methds-reagnts
From: lowreypl@casbah.acns.nwu.edu (Phillip Lowrey)
Subject: RAPD PCR Method
Organization: Northwestern University, Evanston Illinois.
Date: Thu, 30 Jul 1992 02:36:47 GMT

Hello,

	I am interested in a technique known as 'Random Amplified Polymorhic
DNA' (RAPD) as first reported by Williams et al., Nucleic Acids Research 8,
6531-6535 (1990).  If anyone has had any experience with this method I would
appreciate your input.  I am particularly interested in the efficiency of this
technique in the detection of polymorphisms for genome mapping studies.

Thanks for your help.
From: wetsel_r@wums.wustl.edu
Newsgroups: bionet.molbio.methds-reagnts
Subject: RE: S35- met labelling of proteins: URGENT
Date: 30 JUL 92 14:47:24 GMT
Organization: MIT PLASMA FUSION CENTER
NNTP-Posting-Host: msnews.wustl.edu

That's an open question, some additional details would help.  However, we
routinely label cultured cells using the 35S-Trans label from ICN.  We bring up
the volume such that 100 ul equals 1mCi.  We plate our cells in either 24 or 6
well plates at 3-4X10^5 or 1X10^6 cells per well, respectively.  Use met free
 medium with either 0.5 % BSA or 5% dialyzed calf serum.  Depending upon the
cell type, we will add 0.250 - 0.500 uCi per ml of labeling media and incubate
that on the cells anywhere from 2-24 hours, normally 4 hours at 37'C works very well for most continuous cell lines.  I should note, prior to adding the
labeled media to the cells, we will wash the cells 2-3 times in HBSS.  This
works very well in our hands for both adherent and non-adherent cells.  We oftenwill then use the corresponding supernatant and cellular lysate in
immunoprecipitations - I'll assume that is what you're doing?  Post back
if you need more help... best of luck...

David
Organization: Queen's University at Kingston
Date: Tuesday, 28 Jul 1992 12:15:04 EDT
From: <BACKD@QUCDN.QueensU.CA>
Newsgroups: bionet.molbio.methds-reagnts
Subject: Re: Smiles on Southerns
Distribution: bionet

Check very carefully to see if your gel is of uniform thickness. If the agarose
 is poured too hot ( > 55C) the plexiglass will warp and the gel will
be thinner in the center. This will give a "nice" smile.
Don Back
Department of Biochemistry
Queen's University
Kingston, Ontario. CANADA
Newsgroups: bionet.molbio.methds-reagnts
From: mingo@world.std.com (Charles H Mingo)
Subject: Desperately Seeking _Biotechniques_
Organization: The World Public Access UNIX, Brookline, MA
Date: Sun, 26 Jul 1992 13:39:48 GMT

 	I was wondering if anyone could assist me in finding an article
in a free journal for a friend without net access.
 
	I am looking for an article in _Biotechniques_ #6:632 (1988)
by C. Chen & H. Okayama.  The journal _Biotechniques_ is not distributed
in Canada, and is hard to find as libraries cannot order it through the
usual channels.
 
	If anyone could send a copy to my address below I would be most
appreciative.

-- 
______________________________________________________________________________
Charlie Mingo					Internet: mingo@well.sf.ca.us 
2209 Washington Circle #2				  mingo@cup.portal.com
Washington, DC  20037	    AT&T:  202/785-2089 	  mingo@world.std.com
From: wetsel_r@wums.wustl.edu
Newsgroups: bionet.molbio.methds-reagnts
Subject: RE: Separation of DNA fragments.
Date: 30 JUL 92 13:38:38 GMT
Organization: MIT PLASMA FUSION CENTER
NNTP-Posting-Host: msnews.wustl.edu

Yes.  Jack up the percent agarose in your gel up to between 3 and 4%.  It makes
for one stiff gel ::grin::  Our lab has used this to separate PCR products
derived from "allelic PCRs" where one allele had a 28 bp deletion in comparison
to the other allele.  However, I should add that the PCR products weren't much
over 500 bp.  A 3 or 4% gel will resolve the difference and separate the 281
from the 271 bp markers in the phi-X-174X HaeIII molecular weight markers.  At
3.3-3.4 kb you will probably have to run the gel for a while, like overnight, to
3.3-3.4 kb you will probably have to run the gel for a while, like overnight, toget the resolution and separation you need for purification...  Good luck...

David
From: wetsel_r@wums.wustl.edu
Newsgroups: bionet.molbio.methds-reagnts
Subject: RE: ethanol precipitation
Date: 22 JUL 92 10:01:20 GMT
Organization: MIT PLASMA FUSION CENTER
NNTP-Posting-Host: msnews.wustl.edu

Andrews conclusion is also supported in Bernard Perbal's textbook, "A practical
guide to molecular cloning", 2nd Ed. in which he states that temperature has
no effect on the recovery of DNA in an EtOH precipitation.  What DOES make a
major difference is the time of centrifugation.  30 mins at room temp is a
whole lot better that 10 minutes at 4'C when it comes to percentage of DNA
recovered.  I beleive the figure was something like 90-95% recovery with a
30 minute spin.

David
Newsgroups: bionet.molbio.methds-reagnts
From: kim@m44.unm.edu
Subject: Re: Smiles on Southerns
Date: Wed, 29 Jul 92 18:24:12 GMT
Organization: UNM Cancer Center, Albuquerque, NM
Reply-To: kim@m44.unm.edu

In article <1992Jul29.034016.22825@uniwa.uwa.edu.au>, robert@arbo.microbiol.uwa.oz.au (Robert Coelen) writes:
>In article <d3mmcsa@lynx.unm.edu> kim@m44.unm.edu writes:
>>In article <1992Jul28.110853.24275@gserv1.dl.ac.uk>, MORRIS@ch.unige.cmu ("Mike Morris, Geneva") writes:
                                    
Stuff about salt and temperature and gel migration deleted
 
IBI makes an electroelution device that works by setting up a high salt "trap"
in which the migration of DNA or proteins is so inhibited that they just stay
in the high salt chamber.  This is a V-shaped tunnel in a piece of Plexiglas in
which a high salt buffer is layered at the bottom, and normal electrophoresis
buffer is layered on top.  The gel slice is placed next to one end of the
tunnel and the current is then applied.  DNA migrates out of the gel slice to
be trapped in the high salt layer.

Does this operate on some other principle than high-salt gel smiles?  Has
anyone tried either the real thing from IBI or a home-made copy?

Daniel Kim
Xref: sparky bionet.molbio.methds-reagnts:1563 sci.misc:1397 sci.chem:2816
From: pk03+@andrew.cmu.edu (Paul Karol)
Newsgroups: bionet.molbio.methds-reagnts,sci.misc,sci.chem
Subject: Re: What happens to 32P-labelled compounds?
Date: 28 Jul 92 05:16:19 GMT
Article-I.D.: andrew.0eRIWXq00iUyA1blkI
Organization: Carnegie Mellon, Pittsburgh, PA

Decay of P-32, although producing S-32, will only rarely result in the
replacement reaction.  Of prime concern should be the radiolysis of the
labeled mixture.  The betas will generate many thousands of ions,
radicals, and excited molecules within a millimeter of their
'production'.  These, obviously, are very chemically reactive, and
frequently catalytic too.

Paul J. Karol
Nuclear Chemist
(but not Radiation Chemist...a radiation chemist will be able to provide
more detailed information)
From: wetsel_r@wums.wustl.edu
Newsgroups: bionet.molbio.methds-reagnts
Subject: RE: Separation of DNA fragments.
Date: 31 JUL 92 11:37:18 GMT
Organization: MIT PLASMA FUSION CENTER
NNTP-Posting-Host: msnews.wustl.edu

>jaf-  I have to disagree with a posted followup that says to use a 4% 
>gel; this would work with a smaller fragment but not with 3.4k.
> 
>We have had good luck by digesting the set with an enzyme that cuts in the
>middle of the piece you *don't* want, effectively removing it from the
>piece you do.  It's surprising how often a good enyme for this can be
>found.
---- text deleted ----
> 
>dennis
------

Yes, I agree with Dennis.  However, we have used both techniques to 
separate DNA fragments, high percentage gel and restriction enzyme to 
remove the contaminating band.  Dennis' approach is the better for the 
reasons he stated.  I assumed that enzymes were not a viable option (which 
has occured with our research) leaving us the only option of high 
percentage gel.

If you can "remove" or clear the contaminating band out of the way with an 
enzyme, that's your best approach.

David
From: ak990140@SPECIALK.IAIMS.BCM.TMC.EDU (Arthur Kania)
Newsgroups: bionet.molbio.methds-reagnts
Subject: S35- met labelling of proteins: URGENT
Date: 30 Jul 92 18:57:24 GMT
Reply-To: ak990140@SPECIALK.IAIMS.BCM.TMC.EDU (Arthur Kania)
Organization: Baylor College of Medicine, Houston, Tx
Originator: ak990140@SPECIALK.IAIMS.BCM.TMC.EDU



I'm trying to label proteins secreted by cultured cells. I would like to know how much labelled amino acid I should add in order to label adequate amounts. The medium is already met-deficient.
Thanks for Your help.

					AK
From: JOFTOPSM@VTVM1.CC.VT.EDU ("Laura E. Via")
Newsgroups: bionet.molbio.methds-reagnts
Subject: Thermocycling machines
Date: 31 Jul 92 17:38:17 GMT
Distribution: bionet

Dear Netters,

We are thinking of buying a "Thermocycler" device for PCR aplications, but we
can't and won't paid $12,000 for the machine. So my questions are as follows:

1) Has anyone used a Crocodile II from Appligene?  Does it behave well under
heavy use? Any opinions?

2) Would anyone like to recommend a machine? I can spend $5,000, but less
would be much better.  The above Croc. II is about 1/2 that price.

3) I know it is not nice to pan a company's product, but are there machines
I should avoid?

Please respond to JOFTOPSM@VTVM1.cc.vt.edu and I will send a summary to
anyone who requests it. Thanks in advance.

Laura Via
"Always improve lab procedures, but help the new grad students figure out
 what was problematic with the old procedure before allowing them to buy aKIT."
Newsgroups: bionet.molbio.methds-reagnts
From: Xiaodong Shao <shao@umdnj.edu>
Subject: Need help on Northern hyb 
Mailer: Elm [revision: 66.36.1.1]
Reply-To: shao@umdnj.edu
Organization: Indiana University
Date: Thu, 23 Jul 92 12:37:01 EDT

hi,netters.

I have a problem with Northern hyb using oligos probe. the probe
I used was 55-mers labelled with p-32 ATP. The counting was
200,000,000dpm/ug. Prehyb in 6XSSPE, 0.1%SDS, 10XDenhart's,50ug/ml
DNA. Hyb in 6XSSPE, 1%SDS onernight in65C. Washing 3 times In
6XSSPE, 0.1%SDS 15 mins each. Repeat final wash at 65C. and I got
nothing on the membrance. BTW, the membrance I used was MSI MAGNA
Nylen.

I know there were enough mRNA on the membrance and the mRNA I
needed was moderate expression. 

So what's going on there? any suggestion would be appreciated.

thanks in advance.
Newsgroups: bionet.molbio.methds-reagnts
From: MREEBEN@BIOMED.UIO.NO (Mati Reeben)
Subject: Re: Tracer Dyes in Agarose
Organization: The Norwegian EMBnet Node
Date: Mon, 27 Jul 1992 22:36:05 GMT
X-News-Reader: VMS NEWS 1.22

In <3643@fcs280s.ncifcrf.gov> pnh@fcsparc6.ncifcrf.gov writes:

> Hello. For some time I've been using a ficoll based loading
> solution for my agarose gels with both xylene cyanol and
> bromphenol blue added as tracer dyes. Recently, I saw an
> advertisement for a loading dye mix of three dyes used.
> When I called the company which sells these, they would not
> tell me what the dyes are. BUT, they told me that they are
> NOT the two mentioned above...So, I ask if anyone knows of
> any other dyes that can be used for this purpose? In particular
> I'm wondering if anyone uses a dye that migrates faster than the
> bromphenol blue front?
> 
> 
> Paul N. Hengen
(address deleted)

We use orange G (Sigma 1992 Catalog O 3756) in a ficoll based loading buffer we
call orange mix (OM): 20 % Ficoll
                      0.25 % orange G
                      20 mM EDTA

It runs faster than bromphenol blue.

Regards,

Mati Reeben
Newsgroups: bionet.molbio.methds-reagnts
From: waite07@angis.su.oz.au ()
Subject: Re: Sodium phosphate buffer
Reply-To: waite07@angis.su.oz.au ()
Organization: ANGIS, The University of Sydney, Australia
Date: Wed, 22 Jul 1992 02:48:24 GMT



	When reference is made to sodium phosphate buffer of a particular pH,
what is meant, is a specific ratio of the mono- and di-sodium salts which gives
a correspondingly specific pH value. The ratios can be obtained from;

	Dawson, R. M. C., Elliot, D. C., Elliot, W. H. and Jones, K. M.,
	(1986). Data for biochemical research. 3rd ed. 432. Clarendon
	Press, Oxford.

	In case this reference can not be found, I include the relevant table;

	pH, 25 deg. C	x ml 0.2M Di-sodium	y ml 0.2M mono-sodium
	--------------------------------------------------------------
	5.8		4.0			46.0
	6.0		6.15			43.85
	6.2		9.25			40.75
	6.4		13.25			36.75
	6.6		18.75			31.25
	6.8		24.5			25.5
	7.0		30.5			19.5
	7.2		36.0			14.0
	7.4		40.5			9.5
	7.6		43.5			6.5
	7.8		45.75			4.25
	8.0		47.35			2.65
	--------------------------------------------------------------
	Original reference;
	 Gomori, after Sorensen, Meth. Enzymol. 1, 143 (1955)

	I usually assume that any dilution of a 0.2M solution will not change
the pH provided that nanopure, or equivalent, water is used.
	I hope this is of some help.

						Alex Jeffries


-------------------------------------------------------------------------------
Alex Jeffries
Plant Virology Lab.
Department of Plant Sciences		waite07@angis.su.oz.au (129.78.25.38)
Waite Agricultural Research Institute
The University of Adelaide		Tel.;  61  8  372 2392
South Australia
Australia
===============================================================================
From: sbhattac@mrc-crc.ac.uk (Dr. Shoumo Bhattacharya B1 061)
Newsgroups: bionet.molbio.methds-reagnts
Subject: E.coli genotypes
Date: 24 Jul 92 08:40:45 GMT
Distribution: bionet

Many thanks to all those who responded to my query on E.coli
genotypes.  To summarise, the info can be had from company catalogues, ATCC
and Barbara Bachmann at Yale.  I wish someone would do an online database
on this though.  Perhaps Genbank?  Then we could put in a request for 
e.g. recA1 + dam-  +  whatever you please and see if any such
bugs are available.
Shoumo Bhattacharya
.


Newsgroups: bionet.molbio.methds-reagnts