From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Mus musculus dihydropyridine sensitive skeletal muscle calcium
Date: 6 Jan 93 07:00:12 GMT
Distribution: bionet
Checksum: 30942 12

LOCUS       MUSCACHB     6010 bp ss-mRNA            ROD       05-JAN-1993
DEFINITION  Mus musculus dihydropyridine sensitive skeletal muscle calcium
            channel mRNA, complete cds.
ACCESSION   L06234
KEYWORDS    dihydropyridine-sensitive calcium channel;
            skeletal muscle calcium channel; voltage-gated calcium channel.
SOURCE      Mus musculus (strain mdg in 129Re/J) immature skeletal muscle cDNA
            to mRNA.
  ORGANISM  Mus musculus
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Rodentia; Myomorpha; Muridae; Murinae.
REFERENCE   1  (bases 1 to 6010)
  AUTHORS   Chaudhari,N.
  TITLE     A single nucleotide deletion in the skeletal muscle-specific
            calcium channel transcript of muscular dysgenesis (MDG) mice
  JOURNAL   J. Biol. Chem. (1992) In press
  STANDARD  full automatic
REFERENCE   2  (sites)
  AUTHORS   Chaudhari,N. and Beam,K.G.
  TITLE     mRNA for cardiac calcium channel is expressed during development of
            skeletal muscle
  JOURNAL   Dev. Biol. (1993) In press
  STANDARD  full automatic
COMMENT     Reference [2] reports features only.
FEATURES             Location/Qualifiers
     mutation        4010
                     /note="The cDNA sequenced is from a naturally occuring
                     mutant mouse strain (mdg). In normal mouse, at nucleotide
                     4010, there is an additional C. The deleted single
                     nucleotide results in a frame-shift.; putative"
     CDS             117..4172
                     /function="voltage gated ion channel"
                     /standard_name="skeletal muscle calcium channel"
                     /citation=[2]
                     /citation=[1]
                     /note="putative"
                     /product="dihydropyridine sensitive calcium channel"
                     /codon_start=1
                     /translation="MEPPSPQDEGLRKKQPKKPVPEILPRPPRALFCLTLQNPLRKAC
                     ISIVEWKPFETIILLTIFANCVALAVYLPMPEDDNNTLNLGLEKLEYFFLIVFSIEAA
                     MKIIAYGFLFHQDAYLRSGWNVLDFIIVFLGVFTVILEQVNIIQTNTAPMSSKGAGLD
                     VKALRAFRVLRPLRLVSGVPSLQVVLNSIFKAMLPLFHIALLVLFMVIIYAIIGLELF
                     KGKMHKTCYFIGTDIVATVENEKPSPCARTGSGRPCTINGSECRGGWPGPNHGITHFD
                     NFGFSMLTVYQCISMEGWTDVLYWVNDAIGNEWPWIYFVTLILLGSFFILNLVLGVLS
                     GEFTKEREKAKSRGTFQKLREKQQLEEDLRGYMSWITQGEVMDVDDLREGKLSLDEGG
                     SDTESLYEIEGLNKIIQFIRHWRQWNRVFRWKCHDLVKSKVFYWLVILIVALNTLSIA
                     SEHHNQPLWLTHLQDVANRVLLTLFTIEMLMKMYGLGLRQYFMSIFNRFDCFVVCSGI
                     LEILLVESGAMSPLGISVLRCIRLLRLFKITKYWTSLSNLVASLLNSIRSIASLLLLL
                     FLFIIIFALLGMQLFGGRYDFEDTEVRRSNFDNFPQALISVFQVLTGEDWNSVMYNGI
                     MAYGGPTYPGVLVCIYFIILFVCGNYILLNVFLAIAVDNLAEAESLTSAQKAKAEERK
                     RRKMSKGLPDKSEEERATVTKKLEQKSKGEGIPTTAKLKIDEFESNVNEVKDPYPSAD
                     FPGDDEEDEPEIPVSPRPRPLAELQLKEKAVPIPEASSFFIFSPTNKIRVLCHRIVNA
                     TWFTNFILLFILLSSAALAAEDPIRADSMRNQILEYFDYVFTAVFTVEIVLKMTTYGA
                     FLHKGSFCRNYFNILDLLVVAVSLISMGLESSAISVVKILRVLRVLRPLRAINRAKGL
                     KHVVQCVFVAIRTIGNIVLVTTLLQFMFACIGVQLFKGKFYSCNDLSKMTEEECRGYY
                     YIYKDGDPTQIELRPRQWIHNDFHFDNVLSAMMSLFTVSTFEGWPQLLYKAIDSNEED
                     TGPVYNNRVEMAIFFIIYIILIAFFMMNIFVGFVIVTFQEQGETEYKNCELDKNQRQC
                     VQYALKARPLRCYIPKNPYQYQVWYVVTSSYFEYLMFALIMLNTICLGMQHYNQSEQM
                     NHISDILNVAFTIIFTLEMVLKLIAFKPRAYFGDPWNVFDFLIVIGSIIDVILSEIDD
                     PDESARISSAFFRLFRVMRLVKLLNRAEGVRTLLWTFIKSFQALPYVALLIVMLFFIY
                     AVIGMQMFGKIAMVDGTQINRNNNFQTFPQAVLLLLGVPQGKPGKRSCWPAAMGSCVT
                     RSLTTHPGRSIHAAPTSPTTTSSVSTCSAPS"
BASE COUNT     1409 a   1686 c   1588 g   1327 t
ORIGIN      
        1 gtctgcctgc ctcagcagat aggctcagtc aggtccagtc cccaaggtgg gggcactggg
       61 gacacaggga ggagagggca gtgggctggg ggaagagagg ggtgtcaggg aaagccatgg
      121 agccgccctc accccaggac gagggcctga ggaagaagca gccgaagaaa cctgttcctg
      181 agattctgcc gaggcccccg cgggccttgt tctgcctgac tctacagaac cccctgagga
      241 aggcctgcat cagcatcgtg gaatggaaac ccttcgagac catcatcttg ctcaccatct
      301 ttgccaactg tgtggccctg gcagtgtacc tgcccatgcc agaggatgac aacaacactc
      361 tgaacctcgg cctggagaaa ctggagtact tcttcctcat tgtcttctcc attgaagccg
      421 ccatgaagat catagcctac ggcttcctat tccatcagga tgcatacctg cgcagtggct
      481 ggaacgttct ggacttcatc attgtctttc tgggggtctt cactgtgatt ctggaacaag
      541 ttaacatcat tcaaaccaac acggccccca tgagcagcaa aggagccggc ttggatgtga
      601 aggcccttcg agcattccgc gtgctcagac ccctgcggct ggtgtcgggg gtgcccagtc
      661 ttcaggtggt cctcaactcc atcttcaagg ccatgctccc cctgttccac attgccctgc
      721 tcgtcctctt catggtcatc atctacgcca tcattgggct ggagctcttc aaaggcaaga
      781 tgcacaagac ttgctacttc attgggacag acattgtagc cacagttgag aatgagaaac
      841 catcaccctg tgctagaact ggctctgggc gtccctgcac catcaatggc agcgagtgcc
      901 gaggaggttg gccaggaccc aaccacggca tcacccactt cgacaacttt ggcttctcta
      961 tgctcactgt gtatcaatgc atctccatgg aggggtggac tgatgtcctg tactgggtca
     1021 acgatgccat cgggaatgag tggccctgga tctactttgt caccctcatt ctgctgggct
     1081 ccttcttcat cctcaacctg gtgctgggtg tcctgagtgg agaattcacc aaagagcgag
     1141 agaaggccaa gtccagggga accttccaaa agctccgaga gaaacagcag ctggaggagg
     1201 acctccgtgg ttacatgagc tggatcacac agggcgaggt catggacgtg gacgacttga
     1261 gagaaggcaa gctgtctttg gatgaagggg gatcagacac agagagcttg tatgaaatcg
     1321 aaggcttgaa caaaatcatc cagttcattc ggcactggcg acagtggaat cgcgtcttcc
     1381 gctggaagtg ccatgaccta gtgaaatcca aggtcttcta ttggctggtg atcctgatcg
     1441 ttgccctaaa caccctgtcc attgcctcgg agcaccacaa ccagccactc tggttgaccc
     1501 atttgcaaga tgttgccaac cgcgtgttgc tgaccctctt caccatcgaa atgctcatga
     1561 agatgtacgg gctgggcctg cgccagtact tcatgtccat cttcaaccgc ttcgactgct
     1621 tcgtggtgtg cagcggcatc ctggagatcc tgctggtgga gtcgggagcc atgtcgcccc
     1681 tgggcatctc tgtgctccgc tgtatccgcc tcctgaggct cttcaagatc accaagtact
     1741 ggacatcgct gagcaacctg gtggcctcac tgctcaactc catccgctcc attgcttccc
     1801 tcttgctgct gctctttctc ttcatcatca tctttgccct gctgggcatg cagctctttg
     1861 gaggcagata tgactttgaa gacacagaag tacgacgaag caactttgac aacttccccc
     1921 aggccctcat cagtgtcttc caggtcctga cgggtgaaga ctggaactct gtgatgtaca
     1981 atgggatcat ggcttacgga ggtccgacct accctggtgt gctggtgtgc atttatttca
     2041 tcatcctttt cgtctgtggc aactacatcc ttctcaacgt cttcctggct attgctgtgg
     2101 acaacctggc agaggcagag agtctgacat ctgcccaaaa ggccaaggct gaggagagaa
     2161 agcgcaggaa gatgtcgaaa gggctcccag acaagtcaga agaggagagg gccacggtga
     2221 ccaagaaact ggagcagaag tccaagggtg aaggcatccc caccaccgcc aagctgaaaa
     2281 tcgatgagtt tgaatcaaac gttaatgagg tgaaagaccc ctacccttcg gctgacttcc
     2341 caggggacga tgaggaagat gagcccgaga tcccagtgag cccccgacca cgccctctgg
     2401 ccgagctgca gctgaaagag aaagcagtgc ccatcccgga ggctagctcc ttcttcatct
     2461 tcagtcccac caacaagatc cgtgtcctgt gtcaccgcat tgtcaatgcc acctggttca
     2521 ccaacttcat cctgctcttc atcctgctca gcagtgccgc cctggccgct gaggatccca
     2581 tccgggccga ctccatgagg aatcagatcc tcgagtattt tgattatgtg ttcactgctg
     2641 ttttcacggt ggagattgtc cttaagatga ccacctatgg agccttcctg cacaaaggct
     2701 ccttctgccg caactacttc aacatcctgg acctgctggt ggtggctgtg tccctcatct
     2761 ccatgggcct cgagtccagc gccatctccg tggtgaagat cttaagggtt ctgagagtgc
     2821 tgagaccact ccgggccata aacagagcca aagggttgaa gcacgtggtg cagtgcgtgt
     2881 ttgttgccat tcgcaccatc gggaacatcg tgctggtcac cacgctcctg cagttcatgt
     2941 ttgcgtgcat tggagtgcag cttttcaagg gaaagttcta cagctgcaat gacttatcca
     3001 agatgactga agaggaatgc aggggctact actacatata caaggatgga gaccccactc
     3061 agatagagct gcgtccccgc cagtggatac acaatgactt ccactttgac aatgtactct
     3121 ccgctatgat gtcactcttc acggtctcca ccttcgaggg ttggcctcag ctactgtaca
     3181 aggccataga ctcaaatgag gaggacaccg gccctgtcta caacaaccga gtggagatgg
     3241 ccatcttctt cattatctac atcatcctca ttgccttctt catgatgaac atctttgtgg
     3301 gctttgtcat cgttaccttc caggaacagg gagagactga gtataagaac tgtgagctcg
     3361 acaagaacca gcgccaatgc gtacagtatg ccctgaaggc ccgcccactg aggtgttaca
     3421 tccccaaaaa cccataccag tatcaggtgt ggtatgtcgt cacttcctcc tactttgaat
     3481 acctgatgtt tgctctcatc atgctcaata ctatctgcct gggcatgcag cattacaacc
     3541 agtcggaaca gatgaaccac atctcagaca ttctcaacgt ggctttcacc atcatcttca
     3601 ccctggagat ggtcctcaag ctcatagctt tcaagcccag ggcctatttt ggagatcctt
     3661 ggaatgtgtt tgacttccta atcgtcatcg gcagcatcat tgacgtcatc ctgagcgaga
     3721 tcgatgaccc agatgagagc gcccgtatct ccagtgcctt cttccgcctg ttccgggtca
     3781 tgagactggt caagctgctg aaccgggcgg agggtgtgcg caccctgctg tggacattca
     3841 tcaagtcctt ccaggccctg ccgtacgtgg ctttgctgat cgtgatgctc ttcttcatct
     3901 acgcggtcat cggcatgcag atgttcggga agatcgccat ggtggacggg acgcagataa
     3961 accggaacaa caacttccag actttcccac aggccgtgct gctgctctta ggtgtgccac
     4021 aggggaagcc tggcaagaga tcctgctggc ctgcagctat gggaagctgt gtgacccgga
     4081 gtctgactac gcacccgggg aggagcatac atgcggcacc aacttcgcct actactactt
     4141 catcagtttc tacatgctct gcgccttcct gatcatcaac ctctttgtgg ctgtcatcat
     4201 ggacaacttt gattacctca caagggactg gtccattctg ggccctcacc acctggacga
     4261 gttcaaggcc atttgggctg agtacgaccc ggaggctaag ggcaggatca agcacctgga
     4321 tgtggtgacc ctgctgagga ggattcagcc ccctctgggc tttgggaagt tctgcccaca
     4381 cagggtagca tgtaagaggc tggtgggcat gaacatgccc ctgaacagcg atggcacagt
     4441 cacctttaat gccacactat ttgccctggt gcgcacagcc ctcaagatca agacagaagg
     4501 caactttgaa caagctaacg aggagctgag agccatcatc aagaagatct ggaagagaac
     4561 cagcatgaag ctcttggacc aagtcatccc tccaatagga gatgatgagg tgacagtagg
     4621 gaaattctat gccacattcc tcatccagga gcacttccgg aagttcatga agcgccagga
     4681 ggaatattat gggtatcgac ccaaaaagga cacagttcag atacaggctg gattacggac
     4741 catagaggaa gaggcagctc ctgagatcca ccgtgccatc tctggagacc cgacagcaga
     4801 ggaggaactg gagagggcca tggtagaggc tgccatggag gaggggatat tccggaggac
     4861 gggaggcctg tttggtcaag tggacaactt cctggaaagg accaactctc tgccccctgt
     4921 catggccaac caaagacccc tccaatttgc tgaaatagag atggaagagc tggagtcacc
     4981 agtcttcttg gaggacttcc ctcagaaccc aggcacccac cctcttgctc gtgccaatac
     5041 caacaacgcc aatgccaatg ttgcctatgg caacagcagc catagaaaca acccagtgtt
     5101 ttccagcatc tgctatgaac gggagttctt gggagaggcg gacatgcctg ttaccagaga
     5161 aggacccctc agtcagccct gcagcggctc aggacctcac agcagatctc atgtggacaa
     5221 actgaaaaga ccgatgaccc agagaggaat gcctgaaggc caggtgcctc cttccccgtg
     5281 ccagctctcc caggcagagc atcctgtgca gaaagaggga aagggcccca cgtcacggtt
     5341 tctggaaaca cccaacagca ggaactttga ggagcacgtt cccaggaatt cggctcacag
     5401 gtgcacggct ccagctactg ccatgctgat ccaggaggct ctggttcgag gaggactaga
     5461 ctccttagca gcagatgcta acttcgtcat ggcaacaggc caggccctgg cagatgcctg
     5521 ccagatggaa ccagaggaag tcgaggtggc agcaacagag ctactgaagc aagaatcccc
     5581 agaggcgggg ccgtgcctgg gagccttgag cctcaggtcc tcccctgggc cgcctgaatc
     5641 tgacgattgg ggctcccaaa cgacccttat tactcccaga tgtgaggcct atacagaatg
     5701 acacaagctt agaaatacca tgaccaaaag ccagaaagtg ctggagtgga gaagtaggtt
     5761 agcccagctt agcagcctcc cctggcccct cagtagccag ctgtatggac tgagtagcgg
     5821 tggtcgggaa ccacctcaac caggcaggag gccatgggtg ggcagggccg gcccttctcc
     5881 tcatcagcaa gaagcaggat tcggcagggc ttgtgtggcc attcacaaag gcctgatcgg
     5941 tgccagggtc tgtctgtcat aaagccactc tatcttgctg gacattaaag tctccaggcc
     6001 tgactttgga
//