From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Bacillus thuringiensis insecticidal protein (cryV) gene, complete
Date: 14 Aug 92 10:00:19 GMT
Distribution: bionet
Checksum: 09616     5

LOCUS       BACCRYVA     2160 bp ds-DNA             BCT       14-AUG-1992
DEFINITION  Bacillus thuringiensis insecticidal protein (cryV) gene, complete
            cds.
ACCESSION   M98544
KEYWORDS    cryV gene; insecticidal protein.
SOURCE      Bacillus thuringiensis (sub_species kurstaki) (library: DSIR732)
            DNA.
  ORGANISM  Bacillus thuringiensis
            Prokaryotae; Firmicutes; Endospore-forming rods and cocci;
            Bacillaceae.
REFERENCE   1  (bases 1 to 2160)
  AUTHORS   Gleave,A.P., Williams,R. and Hedges,R.J.
  TITLE     Screening by PCR of Bacillus thuringiensis serotypes for the
            presence of CRYV genes and the nucleotide sequence of the CRYV gene
            of Bacillus thuringiensis var. Kurstaki DSIR732
  JOURNAL   Unpublished (1992)
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     CDS             1..2160
                     /product="insecticidal protein"
                     /gene="cryV"
                     /codon_start=1
                     /translation="MKLKNQDKHQSFSSNAKVDKISTDSLKNETDIELQNINHEDCLK
                     MSEYENVEPFVSASTIQTGIGIAGKILGTLGVPFAGQVASLYSFILGELWPKGKNQWE
                     IFMEHVEEIINQKISTYARNKALTDLKGLGDALAVYHDSLESWVGNRNNTRARSVVKS
                     QYIALELMFVQKLPSFAVSGEEVPLLPIYAQAANLHLLLLRDASIFGKEWGLSSSEIS
                     TFYNRQVERAGDYSDHCVKWYSTGLNNLRGTNAESWVRYNQFRRDMTLMVLDLVALFP
                     SYDTQMYPIKTTAQLTREVYTDAIGTVHPHPSFTSTTWYNNNAPSFSAIEAAVVRNPH
                     LLDFLEQVTIYSLLSRWSNTQYMNMWGGHKLEFRTIGGTLNISTQGSTNTSINPVTLP
                     FTSRDVYRTESLAGLNLFLTQPVNGVPRVDFHWKFVTHPIASDNFYYPGYAGIGTQLQ
                     DSENELPPEATGQPNYESYSHRLSHIGLISASHVKALVYSWTHRSADRTNTIEPNSIT
                     QIPLVKAFNLSSGAAVVRGPGFTGGDILRRTNTGTFGDIRVNINPPFAQRYRVRIRYA
                     STTDLQFHTSINGKAINQGNFSATMNRGEDLDYKTFRTVGFTTPFSFLDVQSTFTIGA
                     WNFSSGNEVYIDRIEFVPVEVTYEAEYDFEKAQEKVTALFTSTNPRGLKTDVKDYHID
                     QVSNLVESLSDEFYLDEKRELFEIVKYAKQLHIERNM"
BASE COUNT      741 a    363 c    429 g    627 t
ORIGIN
        1 atgaaactaa agaatcaaga taagcatcaa agtttttcta gcaatgcgaa agtagataaa
       61 atctctacgg attcactaaa aaatgaaaca gatatagaat tacaaaacat taatcatgaa
      121 gattgtttga aaatgtctga gtatgaaaat gtagagccgt ttgttagtgc atcaacaatt
      181 caaacaggta ttggtattgc gggtaaaata cttggtaccc taggcgttcc ttttgcagga
      241 caagtagcta gtctttatag ttttatctta ggtgagctat ggcctaaggg gaaaaatcaa
      301 tgggaaatct ttatggaaca tgtagaagag attattaatc aaaaaatatc aacttatgca
      361 agaaataaag cacttacaga cttgaaagga ttaggagatg ccttagctgt ctaccatgat
      421 tcgcttgaaa gttgggttgg aaatcgtaat aacacaaggg ctaggagtgt tgtcaagagc
      481 caatatatcg cattagaatt gatgttcgtt cagaaactac cttcttttgc agtgtctgga
      541 gaggaggtac cattattacc gatatatgcc caagctgcaa atttacattt gttgctatta
      601 agagatgcat ctatttttgg aaaagagtgg ggattatcat cttcagaaat ttcaacattt
      661 tataaccgtc aagtcgaacg agcaggagat tattccgacc attgtgtgaa atggtatagc
      721 acaggtctaa ataacttgag gggtacaaat gccgaaagtt gggtacgata taatcaattc
      781 cgtagagaca tgactttaat ggtactagat ttagtggcac tatttccaag ctatgataca
      841 caaatgtatc caattaaaac tacagcccaa cttacaagag aagtatatac agacgcaatt
      901 gggacagtac atccgcatcc aagttttaca agtacgactt ggtataataa taatgcacct
      961 tcgttctctg ccatagaggc tgctgttgtt cgaaacccgc atctactcga ttttctagaa
     1021 caagttacaa tttacagctt attaagtcga tggagtaaca ctcagtatat gaatatgtgg
     1081 ggaggacata aactagaatt ccgaacaata ggaggaacgt taaatatctc aacacaagga
     1141 tctactaata cttctattaa tcctgtaaca ttaccgttca cttctcgaga cgtctatagg
     1201 actgaatcat tggcagggct gaatctattt ttaactcaac ctgttaatgg agtacctagg
     1261 gttgattttc attggaaatt cgtcacacat ccgatcgcat ctgataattt ctattatcca
     1321 gggtatgctg gaattgggac gcaattacag gattcagaaa atgaattacc acctgaagca
     1381 acaggacagc caaattacga atcttatagt catagattat ctcatatagg actcatttca
     1441 gcatcacatg tgaaagcatt ggtatattct tggacgcatc gtagtgcaga tcgtacaaat
     1501 acaattgagc caaatagcat tacacaaata ccattagtaa aagctttcaa tctgtcttca
     1561 ggtgccgctg tagtgagagg accaggattt acaggtgggg atatccttcg aagaacgaat
     1621 actggtacat ttggggatat acgagtaaat attaatccac catttgcaca aagatatcgc
     1681 gtgaggattc gctatgcttc taccacagat ttacaattcc atacgtcaat taacggtaaa
     1741 gctattaatc aaggtaattt ttcagcaact atgaatagag gagaggactt agactataaa
     1801 acctttagaa ctgtaggctt taccactcca tttagctttt tagatgtaca aagtacattc
     1861 acaataggtg cttggaactt ctcttcaggt aacgaagttt atatagatag aattgaattt
     1921 gttccggtag aagtaacata tgaggcagaa tatgattttg aaaaagcgca agagaaggtt
     1981 actgcactgt ttacatctac gaatccaaga ggattaaaaa cagatgtaaa ggattatcat
     2041 attgaccagg tatcaaattt agtagagtct ctatcagatg aattctatct tgatgaaaag
     2101 agagaattat tcgagatagt taaatacgcg aagcaactcc atattgagcg taacatgtag
//