From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human mitochondrial (clones lHT2K[1,2,3,6,7,8,9]) acetoacetyl-CoA
Date: 29 Jul 92 10:02:29 GMT
Distribution: bionet
Checksum: 53981     4

LOCUS       HUMMAT01     1072 bp ds-DNA             PRI       29-JUL-1992
DEFINITION  Human mitochondrial (clones lHT2K[1,2,3,6,7,8,9]) acetoacetyl-CoA
            thiolase (ACAT) gene, exon 1.
ACCESSION   D10500 D90476
KEYWORDS    acetoacetyl-CoA thiolase; mitochondria;
            mitochondrial acetoacetyl-CoA thiolase.
SEGMENT     1 of 12
SOURCE      Homo sapiens (individual_isolate Japanese) (library: lEMBL3 human
            genomic library) peripheral blood DNA.
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (sites)
  AUTHORS   Fukao,T., Yamaguchi,S., Kano,M., Orii,T., Fujiki,Y., Osumi,T. and
            Hashimoto,T.
  TITLE     Molecular cloning and sequence of the complementary DNA encoding
            human mitochondrial acetoacetyl-coenzyme A thiolase and study of
            the variant enzymes in cultured fibroblasts from patients with
            3-Ketothiolase deficiency
  JOURNAL   J. Clin. Invest. 86, 2086-2092 (1990)
  STANDARD  full staff_review
REFERENCE   2  (bases 1 to 1072)
  AUTHORS   Kano,M., Fukao,T., Yamaguchi,S., Orii,T., Osumi,T. and Hashimoto,T.
  TITLE     Structure and expression of the human mitochondrial acetoacetyl-coA
            thiolase gene
  JOURNAL   Unpublished (1992)
  STANDARD  full staff_review
COMMENT     Data kindly submitted in computer readable form by:
            Masatsugu Kano
            Department of Pediatrics
            Gifu University
            School of Medicine
            Gifu 500
            Japan
            Phone:  0582-65-1241 x 2819
            Fax:    0582-65-9011
            [1]  sites.
FEATURES             Location/Qualifiers
     exon            819..1060
                     /number=1
                     /standard_name="mitochondrial acetoacetyl-CoA thiolase"
                     /note="minor transcript"
                     /gene="ACAT"
                     /evidence=EXPERIMENTAL
     GC_signal       830..840
                     /gene="ACAT"
     CAAT_signal     852..856
                     /gene="ACAT"
     CAAT_signal     879..883
                     /gene="ACAT"
     exon            894..1060
                     /number=1
                     /standard_name="mitochondrial acetoacetyl-CoA thiolase"
                     /note="major transcript"
                     /gene="ACAT"
BASE COUNT      229 a    280 c    327 g    236 t
ORIGIN
        1 gaattcagac agcatggtgg gtatggcttg tctctgctcc atgatgtctg gggtctcagc
       61 tggagtggtt gaaggttggg gactggaatc acctgaggcc tttttaccca tatttgtatc
      121 tggactgagc ctggaatttg attgcttcaa aatattttca cggctgggtg tgatggctca
      181 cgcctgtaat cccagcactt tgagaggctg agtctggccg tctgcttgag cccaggagtc
      241 agcaaccagc ctaggcaaca tagggagatc tagtctctac gaaaaaaaaa aatacaacaa
      301 tttgctgggc gtggtcgcct gcgcctgtaa tcccagcact ttgggaggcc gaggagggag
      361 gctcacttga gtccaggagt tcaagaccag tctggccaac atggtgaaac cccgtctcca
      421 caaaaaatac aaaaattatc tggttgtgga ggctttcgcc tgtagtctca gctactggga
      481 tggctgggga gggaggatcg cttgagccct gatggtcgag attacagtga gctgtggtca
      541 cgtcactgca ctccagcttg ggtgacagag tgagaccttg tctcaaaaaa aaaaaaaaat
      601 cctaaaatat tttcatgagt ttgtgctggg taggcctaag ggaaacatgc tcagtaaatc
      661 gaaaaatctc ggattacaca taaaccaccg gccccggcag gttagacttg gtggacacgg
      721 gagccagcag cgtgtcaggt gctcgttgac cccagcgcca gtgtctccat ccacgtcctt
      781 cactcgtcaa cctttcctgg cccaggctgc ggtgtcccag cgccaggctc cgccccgccc
      841 ttggctgccg gccaatcgcc gccgactgag aggcgactat tggaggaagc gggatgggcg
      901 gtgcccgcgc cgggccgcta ggggtgcggg gttggggagg aggccgctag tctacgcctg
      961 tggagccgat actcagccct ctgcgaccat ggctgtgctg gcggcacttc tgcgcagcgg
     1021 cgcccgcagc cgcagccccc tgctccggag gctggtgcag gtgagcgggg tt
//