From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Wheat ubiquitin activating enzyme (E1) mRNA, complete cds.
Date: 31 Jul 92 10:02:05 GMT
Distribution: bionet
Checksum: 49182     7

LOCUS       WHTE1B       3588 bp ss-mRNA            PLN       31-JUL-1992
DEFINITION  Wheat ubiquitin activating enzyme (E1) mRNA, complete cds.
ACCESSION   M90664
KEYWORDS    ubiquitin activating enzyme.
SOURCE      Triticum aestivum (strain Augusta) seedling whole seedling cDNA to
            mRNA.
  ORGANISM  Triticum aestivum
            Eukaryota; Plantae; Embryobionta; Magnoliophyta; Liliopsida;
            Commelinidae; Cyperales; Poaceae.
REFERENCE   1  (bases 1 to 3588)
  AUTHORS   Hatfield,P.M. and Vierstra,R.D.
  TITLE     Multiple forms of ubiquitin-activating enzyme E1 from wheat:
            Identification of an essential cysteine by in Vitro mutagenesis
  JOURNAL   J. Biol. Chem. 267, 14799-14803 (1992)
  STANDARD  full staff_entry
FEATURES             Location/Qualifiers
     CDS             186..3347
                     /product="ubiquitin activating enzyme"
                     /gene="E1"
                     /codon_start=1
                     /translation="MLPSKRPSDAAAGDENGRGGDARGPGSGRRRARAAAGAVTAAPQ
                     EIDEDLHSRQLAVYGRETMRRLFASDVLVSGLNGLGAEIAKNLALAGVKSVTIHDVKT
                     VKMWDLSGNFFLSEDDIGKNRAAACVAKLQELNNAVLISALTEELTTEHLSKFQAVVF
                     TDIDLDKAYEFDDYCHNHQPPISFIKSEVCGLFGSVFCDFGPKFTVLDVDGEDPHTGI
                     IASISNDNPALISCVDDERLEFQDGDLVVFSEVHGMTELNDGKPRKVKNARPFSFSIE
                     EDTSNFGIYVKGGIVTQVKEPKVLCFKALRDAMTDPGEVLLSDFSKFERPPVLHLAFQ
                     ALDKFKKDHGRCPAAGCEEDAHSFLKIAAAINEASADRKLDTIDEKLFRQFASGSRAV
                     LNPMAAMFGGIVGQEVVKACSGKFHPLNQFFYFDSVESLPTYPLEPQDLKPSNNRYDA
                     QVSVFGSKLQKKMEEANTFVVGSGALGCEFLKNLALMGVSCSSKGKLTITDDDIIEKS
                     NLSRQFLFRDWNIGQAKSTVAATAASAINPSLHIDALQNRACPDTENVFHDTFWEGLD
                     VVINALDNVNARMYMDMRCLYFQKPLLESGTLGAKCNIQMVIPHLTENYGASRDPPEK
                     QAPMCTVHSFPHNIDHCLTWARSEFEGLLEKTPNEVNSFLSNPAQYAAAMRKAGDAQA
                     RELLERVSECLNKDRCSTFDDCISWARLKFEDYFSNRVKQLTFTFPEDAATSMGAPFW
                     SAPKRFPRALQFSAADQSHLNFIMSASILRAESFGVAIPEWAKDTSKLADVVNKIAVP
                     TFEPKQGVNIVTDEKASNLSSTSVDDVAVIEDLLAKLQEYAKMLLPGFQMKPIQFEKD
                     DDTNFHMDLISGLANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELY
                     KVIAGEHPVEDYRNTFANLALPLFSMAEPVPPKVMKHKETSWTVWDRWSVQGNLTLAE
                     LLQWFADKGLTAYSISCGTSLLYNNMFARHKDRLTKKVVDIAREVAKVDVPEYRRHLD
                     IGVACEDEDENDVDIPLVSVYFR"
BASE COUNT      875 a    912 c    919 g    882 t
ORIGIN
        1 ggggcgcctc cccccttgct catccccctc cctccccccg ccgcccgccc cacgaatcag
       61 taccccctcc cgaagctccc acgccgcccc gcgccgaaac ctagccgacc acgcgtcgcg
      121 cctcctccga tcccttccct cccccgcgcc ggacgaggcc gccgccccac aggggcctgc
      181 gcgccatgct cccgtccaag aggccgtccg acgccgcggc gggggacgaa aacggccgcg
      241 gaggggacgc aagaggccca ggctcgggga gacggagggc gcgggcggcg gcgggggcag
      301 taacggccgc gccgcaggag atcgacgagg acctccacag caggcagctc gccgtgtacg
      361 ggagggagac catgcgccgc ctcttcgcct ccgacgtcct cgtctccggg ctcaacgggc
      421 ttggcgccga aattgcaaag aatcttgccc ttgctggagt caagtctgtc actatacatg
      481 atgtaaaaac tgtgaagatg tgggacttgt ctggcaattt ctttttatct gaggatgaca
      541 ttgggaagaa cagggctgct gcttgtgtag caaagctgca ggagctgaac aatgctgttc
      601 ttatatcagc tctgacagaa gaactaacga cggagcacct gtctaagttc caggctgttg
      661 ttttcactga tatagattta gacaaggctt atgaatttga tgattattgt cacaaccacc
      721 agcctcctat ttcctttatc aaatctgaag tctgtggcct ttttggtagt gtcttttgtg
      781 actttggacc aaagtttact gttcttgatg ttgatggtga agatccgcat actggtataa
      841 ttgcatcgat cagcaatgat aatcctgccc tgatatcctg tgttgatgat gaacggcttg
      901 aattccaaga tggtgatctt gttgttttct ctgaggtcca tggtatgaca gaactgaatg
      961 atggaaaacc aaggaaggtt aaaaatgcaa gaccgttttc atttagcatc gaggaggaca
     1021 caagtaactt tggcatttat gtaaaaggtg gaattgtcac acaagtgaag gaaccaaagg
     1081 tgctatgctt caaggcacta agagatgcca tgacagatcc tggagaagtt cttctgagtg
     1141 acttctcaaa gtttgagcgc ccccctgtgc ttcatctggc atttcaagct ctggacaaat
     1201 ttaagaaaga ccatggacgc tgccctgctg ctggttgtga ggaggatgct catagttttc
     1261 tgaagattgc tgctgctatt aatgaagcct cagctgatcg caagctggac actattgatg
     1321 agaaactatt ccgacagttt gcaagtggtt ctcgagctgt attgaaccct atggctgcga
     1381 tgtttggtgg tattgttggt caagaagttg tgaaggcttg ttcagggaag ttccatcctc
     1441 tcaaccagtt cttctacttt gactctgttg aatccctgcc aacatatccg ttggaacccc
     1501 aagacttgaa gccatcaaac aaccgctatg atgctcaggt ctctgtattt ggttccaagc
     1561 ttcagaagaa aatggaggag gctaatactt ttgttgtagg gtctggtgct cttggatgtg
     1621 aattcctgaa aaaccttgcg ttaatggggg tgtcttgtag cagcaaggga aagttaacca
     1681 taacagatga tgatatcatt gagaaaagta atttaagccg tcaattcttg ttccgtgact
     1741 ggaatattgg acaggcaaag tctactgtgg ctgctacagc cgctagtgct atcaacccca
     1801 gccttcacat tgatgctctc cagaaccgtg cctgtccaga taccgagaat gtcttccatg
     1861 acacattctg ggagggccta gatgttgtca tcaatgcact tgataatgtc aatgccagga
     1921 tgtatatgga catgaggtgc ctgtacttcc agaagccact actggagtca ggtacattgg
     1981 gcgcaaagtg caatattcaa atggtgattc ctcaccttac tgaaaattac ggggcttcaa
     2041 gagatcctcc tgagaagcag gcgcctatgt gcacagtcca ttcttttcca cacaacattg
     2101 atcactgctt gacatgggct cgttcagagt ttgagggttt gctcgagaaa acgccaaatg
     2161 aagtgaactc ttttctgtct aaccctgctc aatatgctgc tgcaatgagg aaggcaggtg
     2221 atgctcaagc aagagaattg cttgaacgtg tctctgagtg tcttaacaag gaccggtgca
     2281 gtacatttga tgattgcata agctgggccc gactgaaatt tgaggattat ttctcaaacc
     2341 gcgtgaagca gctcacattc acttttcctg aagatgctgc cactagtatg ggtgctcctt
     2401 tctggtctgc ccccaagcgc ttcccccgtg cactgcagtt ttcagctgct gatcaatctc
     2461 accttaactt catcatgtct gcttcgatat tgagagcgga gtcatttggg gttgctatac
     2521 ctgaatgggc aaaggatact agtaagctgg ctgatgtagt aaacaaaatt gcagttccta
     2581 cgtttgagcc aaagcaaggg gttaatattg tgacagatga gaaggcatcg aatctttcca
     2641 gcacctcagt tgatgatgtc gccgttattg aagatcttct ggctaagttg caagaatatg
     2701 ccaagatgct acttccagga ttccaaatga aacctatcca atttgagaag gatgatgaca
     2761 ccaacttcca catggattta atatccggat tagcaaacat gcgtgcaagg aactacagca
     2821 tcccagaggt cgacaagctg aaggccaagt tcatcgcagg caggatcatc ccggccattg
     2881 caacctcaac cgccatggcc acaggactcg tgtgcctcga gctgtacaag gtcatcgccg
     2941 gcgagcaccc cgtcgaggac taccgcaaca cattcgcgaa cctcgccctc ccactcttct
     3001 cgatggccga gccggtcccg cccaaggtca tgaagcacaa ggagacgagc tggaccgtgt
     3061 gggaccggtg gtccgtccag ggcaacctca ccctcgccga gctcctgcag tggttcgccg
     3121 acaagggcct caccgcctac agcatctcgt gcggcacctc cctgctgtac aacaacatgt
     3181 ttgcgaggca caaggaccgg ctgaccaaga aggtggtcga catcgccagg gaggtggcca
     3241 aggtggacgt ccccgagtac cggaggcacc tggacatcgg cgtggcctgc gaggacgagg
     3301 acgagaacga cgtcgacatc cccctcgtgt cggtttactt ccgctagtct gggtctcgag
     3361 ataagaagcc ttgccgcatc tccagaactc tgttgtctcg cgaaagccac tgatgtagta
     3421 gtagtttagc cgccgctctc gcgctgatag ctctatcatg gtcacctaag atgttctctc
     3481 tttgtggtat actggtccta cagttatctg ctggttggtc gcagtcaaat agttggagtt
     3541 ggaggaatga acaacagaca catcggttac ggttaaaaaa aaaaaaaa
//