From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Drosophila melanogaster dihydroorotate dehydrogenase (dhod) gene,
Date: 1 Sep 92 10:00:59 GMT
Distribution: bionet
Checksum: 15023     8

LOCUS       DRODHORO     3078 bp ds-DNA             INV       01-SEP-1992
DEFINITION  Drosophila melanogaster dihydroorotate dehydrogenase (dhod) gene,
            complete cds.
ACCESSION   L00964
KEYWORDS    dihydroorotate dehydrogenase.
SOURCE      Drosophila melanogaster (strain Su(b) lab strain) (library: EMBL4)
            mature adults DNA.
  ORGANISM  Drosophila melanogaster
            Eukaryota; Animalia; Metazoa; Arthropoda; Uniramia; Insecta;
            Pterygota; Neoptera; Holometabola; Diptera; Brachycera;
            Cyclorrhapha; Schizophora; Drosophiloidea; Drosophilidae.
REFERENCE   1  (sites)
  AUTHORS   Jones,W.K., Kirkpatrick,R. and Rawls,J.M.
  TITLE     Molecular cloning and transcript mapping of the dihydroorotate
            dehydrogenase dhod locus of Drosophila melanogaster
  JOURNAL   Mol. Gen. Genet. 219, 397-403 (1989)
  STANDARD  full staff_review
REFERENCE   2  (bases 1 to 3078)
  AUTHORS   Rawls,J., Kirkpatrick,R., Yang,J. and Lacy,L.
  TITLE     The dhod gene and the deduced structure of mitochondrial
            dihydroorotate dehydrogenase in Drosophila melanogaster
  JOURNAL   Unpublished (1992)
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     CDS             join(1136..1183,1238..2359)
                     /EC_number="3.5.2.3"
                     /product="dihydroorotate dehydrogenase"
                     /gene="dhod"
                     /codon_start=1
                     /translation="MDQDHLKNAKNATRRVGRLRSLGIVTVGGAALVAGITAYKNQDQ
                     LFRTFVMPAVRLLPAEASHQLAVLACKYRLCPVSQYHDDQNLHTSFFGRMLSNPIGIA
                     AGFDKNAEAVDGLQDLGFGFIEVGTVTPAAQEGNPKPRVFRLTEDKAIINRYGFNSDG
                     HQAVLQRLRLLRKKENFNGVVGVNLGRNKTTMSPIADYVQGVRVFGPVADYLVINVSS
                     PNTKGLRDMQSKEKLRELLEQVNDTKSSLDKNKNVPILLKLSPDLSLDDMKDIVWVIK
                     RKKSRVDGLIVSNTTVSRENIEKNKLAEETGGLSGPPLKARSTEMIAQMYQLTDGKIP
                     IIGVGGVASGYDAYEKIEAGASYVQIYTALVYEGPALVEDIKAGAVGADHTAGSH"
                     /citation=[1]
                     /citation=[2]
     exon            1129..1183
                     /number=1
                     /note="based upon primer extension and S1 protection
                     analysis of embryonic RNA"
                     /gene="dhod"
                     /evidence=EXPERIMENTAL
                     /citation=[2]
     intron          1184..1237
                     /number=1
                     /note="based upon primer extension and S1 protection
                     analysis of embryonic RNA, plus sequencing of embryonic
                     cDNA clones"
                     /gene="dhod"
                     /evidence=EXPERIMENTAL
                     /citation=[2]
     exon            1238..2529
                     /number=2
                     /note="based upon sequencing embryonic and adult cDNA
                     clones"
                     /gene="dhod"
                     /evidence=EXPERIMENTAL
                     /citation=[2]
     old_sequence    replace(2281..2284,"agctg")
                     /citation=[1]
     polyA_site      2529
                     /note="based upon sequencing of embryonic and adult cDNA
                     clones"
                     /evidence=EXPERIMENTAL
                     /citation=[1]
                     /citation=[2]
BASE COUNT      830 a    697 c    790 g    761 t
ORIGIN
        1 aagcttcggc agagaccgcc cttcgtttac cagatattac attgaaagta gaagtacaat
       61 atagtgttta tatgctatgt aatcttacgc tagtttaaca ataaattaca tgtacacaga
      121 agctgctttg ggtggcttgg ttgggggggt agcaggcatc acaagttggt ggcttcgagt
      181 cagacatccg acttttgatt gagggccgca tagcccactg ggatctcctt catctcggtc
      241 tgcaggcaga gaaactcgcc gcctatgcac attattgtgc cggtgatgac attcagtagc
      301 caccacgatg cgttcgcggg aaaggagcgg ttgtaccacc agcagatcag caaatgcagc
      361 acgtggaagg tgcaactgcc atgaaaggga agcatgagtt gtaaaagcaa aagattaagt
      421 gtgtgcacta gctataagcc cacctaaagt ccagacagag cttggctctt ctcacaatca
      481 ccacagtgcc agggaggcta agaacgcatt taaaacaaat gcgcagatca ccaggcggcc
      541 acccatgtcg tagatgtgga tctcctttaa gcaacattaa atcaaagtaa ggagaggggt
      601 ttccatgata tatggtatat agcatatggc atacatggta ttcgaacaag tgatccagac
      661 tatagttgtc cccggacaac ttgttggcca cgaagacgag taggccaagg gtgaagtaca
      721 cgcagaactg catggacaca atctgtgagg acagcagcgt gggatcccac tgcgtgttgc
      781 gaaaggtgcc gcccttcatg gttgcctcgg gtggccaaat cagcgtttag agaacgaaat
      841 ttctaatttt tagctggcca taacaaactg actcaaccat tagaggtgac actggaggac
      901 tatcgattct cttcggcatg gttgtgaata gaaggggttc atttgctaca cagaaaaaaa
      961 agaaaattat tgatgcttgc cgtagtaact atatatcgca tgagttttcc ttcaaaatgg
     1021 ccagacttac tcccaaattg aattttcgtt aaaactctag tacatgcatt tactttttta
     1081 catattatac ttttaatact ctatcgggta caatcgatat ccggcgatag tcgtcatgga
     1141 tcaggatcac ttaaagaatg ctaaaaacgc cacgcggaga gttgtgagta aagtacggct
     1201 taaacgggac cggattacag aatgattccg cccacagggt cgtttgcggt cgctgggaat
     1261 cgttacagtc ggaggagctg ccttggtggc gggcattacg gcatacaaga accaggacca
     1321 gttgttccgg accttcgtga tgccagcggt gcgacttctt ccggcggaag ccagccatca
     1381 actggccgtg ctggcgtgca agtaccgcct gtgcccggtt tcacagtacc atgatgacca
     1441 gaacttgcac acgtcgttct ttggccggat gcttagcaat cccattggca ttgcagcggg
     1501 attcgataag aacgcggagg cggtggacgg cctgcaggat ctaggattcg gatttataga
     1561 ggtgggcaca gttaccccgg cggctcagga gggcaatccc aagccgagag tattccggtt
     1621 gaccgaggac aaggccatca ttaatcggta cggatttaat agcgacgggc atcaggccgt
     1681 gctccaacgg ctgcgcttgc tccgcaaaaa ggagaacttc aatggagttg tgggcgtgaa
     1741 tctgggccgg aacaagacca ccatgagccc gatagcggat tatgtacagg gtgttcgggt
     1801 tttcgggccc gtagcagact acctggtcat taatgtgagc agtccgaaca ccaagggcct
     1861 gcgcgatatg cagagcaagg agaagctaag agagctcctg gaacaggtca acgacacgaa
     1921 atcctccctg gataagaaca agaatgtgcc tatactcttg aaactttcgc cggatctatc
     1981 tttggacgac atgaaggaca tcgtgtgggt aatcaagcgc aagaagagcc gcgtggatgg
     2041 cctgatcgtt tcaaacacca cggtgtcgcg ggaaaacatc gaaaagaaca agctggccga
     2101 ggaaactggc ggattaagtg gtcctccact caaggcccgc tccacagaga tgatcgccca
     2161 gatgtaccag ctcaccgatg gaaagattcc catcataggc gtcggcggag tggcttccgg
     2221 ctatgatgca tacgaaaaga tcgaagctgg agcttcctac gtccaaatct acacggcttt
     2281 agtgtacgag ggacccgctc ttgtggaaga cattaaggcc ggagctgtcg gcgctgatca
     2341 cacggctggg tcacactaat gttgcggacg tggtgggcac caacacaagt tctacttgcc
     2401 caaatgaatc aaagatgcca cgatttgaag caagtgctgc attcatttgt gtaaaactaa
     2461 gctgttaaca aacatttctc atgcttttcc tttggtctaa ccaataaatc attaaattct
     2521 tcattgatta actctgagga ctttcattcg cccgctggac tgtgagcact cgtccatcga
     2581 ggaaatgggt gttctggttg ctggcgctgt tgaaggcatc ccgttggctg aaaaccacaa
     2641 agccatagtg cttggacagg cccagttgcc ggtcaaagac cacctccgcg tggccacgtg
     2701 cccgtacttt gagaagtagg tccgcagttc cttgtgctac ctatcgtcca tggcaggttg
     2761 ccgacgaaca atttgtagct gctgcgaaca aatccgctgg acatgattat gtatgtttta
     2821 attttgtggt tttaaatcac tggtgattgt gcatgatttt aaggtggcgc agattggttt
     2881 atgtagcgtc gacttattga cgaaatgtaa cttttcaact agtaaaaact ttaacaaagc
     2941 tgggattaat ttgtgaaaaa tagaaactca aatctcttgg ttttaaagaa ataattattt
     3001 ataggtgaca tttttatatt cgttttatta ccccacctca ctacttttgc acctgcagga
     3061 ctgcttatcg cacgtagt
//