From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Saccharomyces cerevisiae tRNA, Ile gene, partial cds; guanine
Date: 22 Dec 92 22:57:41 GMT
Distribution: bionet
Checksum: 56665 7

LOCUS       YSCGCDA      2903 bp ds-DNA             PLN       30-NOV-1992
DEFINITION  Saccharomyces cerevisiae tRNA, Ile gene, partial cds; guanine
            nucleotide exchange factor subunit gene, complete cds.
ACCESSION   L07115
KEYWORDS    GCD6 gene; guanine nucleotide exchange factor.
SOURCE      Saccharomyces cerevisiae (strain S288C) DNA.
  ORGANISM  Saccharomyces cerevisiae
            Eukaryota; Fungi; Eumycota; Hemiascomycetes; Endomycetales;
            Saccharomycetaceae.
REFERENCE   1  (bases 1 to 2903)
  AUTHORS   Bushman,J.L., Asuru,A.I., Matts,R.L. and Hinnebusch,A.G.
  TITLE     Evidence that GCD6 and GCD7, translational regulators of GCN4, are
            subunits of the guanine nucleotide exchange factor for eIF-2 in
            yeast
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     CDS             <94..>225
                     /evidence="EXPERIMENTAL"
                     /note="tRNA is not required for GCD6 gene function"
                     /product="tRNA, Ile"
                     /codon_start=1
                     /translation="ARVAQWLELRAYSNIRFPKFLCQRPFKQAFKSNATVVGSNPTSS
                     "
     CDS             459..2597
                     /standard_name="eIF-2B"
                     /gene="GCD6"
                     /citation=[1]
                     /note="putative"
                     /product="guanine nucleotide exchange factor subunit"
                     /codon_start=1
                     /translation="MAGKKGQKKSGLGNHGKNSDMDVEDRLQAVVLTDSYETRFMPLT
                     AVKPRCLLPLANVPLIEYTLEFLAKAGVHEVFLICSSHANQINDYIENSKWNLPWSPF
                     KITTIMSPEARCTGDVMRDLDNRGIITGDFILVSGDVLTNIDFSKMLEFHKKMHLQDK
                     DHISTMCLSKASTYPKTRTIEPAAFVLDKSTSRCIYYQDLPLPSSREKTSIQIDPELL
                     DNVDEFVIRNDLIDCRIDICTSHVPLIFQENFDYQSLRTDFVKGVISSDILGKHIYAY
                     LTDEYAVRVESWQTYDTISQDFLGRWCYPLVLDSNIQDDQTYSYESRHIYKEKDVVLA
                     QSCKIGKCTAIGSGTKIGEGTKIENSVIGRNCQIGENIRIKNSFIWDDCIIGNNSIID
                     HSLIASNATLGSNVRLNDGCIIGFNVKIDDNMDLDRNTKISASPLKNAGSRMYDNESN
                     EQFDQDLDDQTLAVSIVGDKGVGYIYESEVSDDEDSSTEACKEINTLSNQLDELYLSD
                     DSISSATKKTKKRRTMSVNSIYTDREEIDSEFEDEDFEKEGIATVERAMENNHDLDTA
                     LLELNTLRMSMNVTYHEVRIATITALLRRVYHFIATQTLGPKDAVVKVFNQWGLLFKR
                     QAFDEEEYIDLMNIIMEKIVEQSFDKPDLILFSALVSLYDNDIIEEDVIYKWWDNVST
                     DPRYDEVKKLTVKWVEWLQNADEESSSEEE"
BASE COUNT      984 a    462 c    587 g    870 t
ORIGIN      
        1 gaattctcag aaagagctat tttttttttt aataaaatta ttagtactaa ttccagtata
       61 aataacaatg ttatttacgt ctctttcgaa aatgctcgtg tagctcagtg gttagagctt
      121 cgtgcttata gcaacattcg gtttccgaag tttctgtgcc aaagaccttt caaacaggcc
      181 tttaaaagca acgcgaccgt cgtgggttca aaccccacct cgagcacttt ctcttttttt
      241 ttcacacgcg tttgcaacat gcccgtatta catgagctta tttgacttta gatggttttc
      301 agttcttttt tttttttttt tttttttcag tagattaatg cgaagagtta aatcaccaat
      361 tcaaagatgg cagatggaca ccttgtggga taagtataaa aataactaaa agtggtcttg
      421 tgttaaaaat agaatagaaa ccactcataa ggcaaaagat ggctggaaaa aagggacaaa
      481 agaaaagtgg actaggcaac catggaaaga actctgatat ggatgttgaa gatcgtctcc
      541 aggccgttgt cttgacagac tcttatgaaa ctaggtttat gccactgaca gctgtcaagc
      601 caaggtgttt gctgccactg gctaacgtac ctctcattga atacacctta gaatttttgg
      661 ctaaggctgg cgtacatgaa gttttcttaa tttgctcttc tcatgccaac caaattaatg
      721 actatattga gaattctaag tggaacttgc cttggtctcc atttaaaatt accaccatta
      781 tgtctccaga agctagatgt acgggtgatg ttatgagaga tctagataat agaggtatca
      841 ttactggaga ttttatttta gtcagtggtg atgtattgac taacatcgat ttcagcaaaa
      901 tgctagaatt tcacaaaaaa atgcatttgc aagataaaga tcacatctcg acaatgtgtt
      961 tgagcaaagc gagtacctat ccaaaaacaa gaactattga gcctgccgca tttgtcttag
     1021 acaaatccac cagtaggtgt atttattacc aagatttacc attaccaagc tctagagaga
     1081 agacttccat tcagattgac ccagaattgt tggataatgt cgatgaattt gtcataagga
     1141 atgacctcat cgattgtaga attgacattt gtacatctca tgtacctttg atatttcaag
     1201 aaaattttga ctaccaatca ttaaggacag actttgttaa aggtgtcatt tcaagcgata
     1261 tattgggaaa gcatatatat gcctatttga cggacgaata tgctgtaaga gttgaaagtt
     1321 ggcaaactta cgacaccatt tctcaagact ttttaggtag atggtgttat cccttggtct
     1381 tagactctaa catacaggac gatcaaacgt attcttatga atcaagacat atatacaagg
     1441 aaaaagacgt tgttttggcc caatcctgta aaattggtaa gtgcactgca attggatcag
     1501 gaacaaaaat cggagagggt acgaaaattg agaattccgt gattggaagg aactgccaaa
     1561 tcggtgaaaa tattagaatc aagaacagtt tcatttggga tgactgtatc atcggaaata
     1621 acagtataat tgaccattca ttaattgcct ctaatgccac gttagggagt aatgtacgcc
     1681 taaatgatgg ctgtataatt ggtttcaacg ttaaaattga tgataatatg gatttagata
     1741 gaaacacaaa aatatctgcc agtccattaa aaaatgccgg ctcaagaatg tatgataatg
     1801 aaagcaatga gcagtttgac caagaccttg atgatcagac actagccgtt tctattgttg
     1861 gagataaggg tgttggttat atttacgaaa gcgaggtgtc tgacgatgaa gatagttcta
     1921 cagaagcctg caaagaaata aacactttga gtaaccaatt agatgagtta tacttaagtg
     1981 acgattcaat ttcttccgcc actaaaaaga caaagaaaag aagaacgatg tctgtcaata
     2041 gtatatacac ggatagagaa gaaattgatt ctgaatttga ggacgaagat tttgagaaag
     2101 aaggtattgc caccgtggag cgtgctatgg aaaacaatca tgatcttgac acagcattat
     2161 tagaattgaa caccttgaga atgagtatga acgtgacata tcatgaggtg agaatagcaa
     2221 caataactgc attattgaga agagtttacc actttattgc aactcaaaca ttaggtccca
     2281 aggacgctgt ggtgaaggtt tttaatcagt ggggactgtt gttcaagaga caagcctttg
     2341 atgaagaaga gtatattgat ttgatgaaca tcataatgga aaaaattgta gaacagagtt
     2401 ttgacaaacc ggatttgatt ctatttagtg cattggtttc tctatacgat aatgacataa
     2461 ttgaggagga tgtcatttat aaatggtggg ataatgtttc tactgaccct cgctatgatg
     2521 aagtcaagaa attaactgta aagtgggttg agtggttaca gaatgctgac gaagaatctt
     2581 cctcagaaga ggaataaaag tccaccgagt accctgcata atcatgtatt accataaaaa
     2641 ggctttaata tgagaaaaag gtgagcgcta tatagattta gtaatttaca tgttacagaa
     2701 gcaaaaagca aatatatata tattttttat catattagtt tttatcctca gtagtactac
     2761 tgaaataata atctcagatt tttttttttc acttgccgag ggaccttgtc taagtggcaa
     2821 agaactgaaa aaaaaaaaaa ttgaaatgcg gtggtatcac atgaagagta aataacttaa
     2881 gcaaaagaga ggccactgag aga
//