From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: P.americana mRNA for polygalacturonase
Date: 28 Dec 92 21:25:46 GMT
Distribution: bionet
Checksum: 35193 4

LOCUS       AVOPOLYGU    1755 bp ss-RNA             PLN       01-DEC-1992
DEFINITION  P.americana mRNA for polygalacturonase
ACCESSION   X66426
KEYWORDS    polygalacturonase.
SOURCE      Persea americana RNA.
  ORGANISM  Persea americana
            Eukaryota; Plantae; Embryobionta; Magnoliophyta; Magnoliopsida;
            Magnoliidae; Laurales; Lauraceae.
REFERENCE   1  (bases 1 to 1755)
  AUTHORS   Dopico,B., Lowe,A.L., Wilson,I.D., Merodio,C. and Grierson,D.
  TITLE     Fruit mRNAs and their expression during ripening and low
            temperature storage
  JOURNAL   Plant Mol. Biol. (1992) In press
  STANDARD  full automatic
COMMENT     *source: strain=cv Hass; *source: stage=ripening fruit; *source:
            tissue=pericarp; *source: clone_library=cDNA; *source: clone=SBA24;

               From EMBL    entry PAPOLYGU;  dated 14-OCT-1992.
FEATURES             Location/Qualifiers
     CDS             49..1437
                     /EC_number="3.2.1.15"
                     /product="polygalacturonase"
                     /codon_start=1
                     /translation="MALTRLLLPISILWFCFYSSHTILQKDPLLICVNGDPGFDQRAY
                     PTYFGPILDEFSSIMGFEPSILSLERFNPVGGPETSPDTDISVDDFGARGDGTDDTKA
                     FEKAWKDACSSGSVLIVPENKNYLLKQITFSGPCKSDLRVKIRGTIEASSDQSDWVGH
                     NRKRWIEFEDISNLTLEGGGTSNGNGETWWDSSCKRKKSLPCKSAPTALTFRSCKNLI
                     VSDLSIKDSQKMHLSFDKCQDVIASNLMVTAPEHSPNTDGIHITGTQRIHVMNSVIGT
                     GDDCISIESGSKMVIATNITCGPGHGISIGSLGDRNSEAHVSGVLVDGGNLFDTTNGL
                     RIKTWQGGSGSAKNIKFQNIVMHNVTNPIIIDQYYCDSKDPCPEQESAVKVSNVAYMN
                     IRGTSASEVAVKFDCSKSSPCQGYIVGNINLVGNGGKETTMSCSNIVQGLLREGLSTF
                     LFMKRRVHECSY"
BASE COUNT      544 a    336 c    393 g    482 t
ORIGIN      
        1 aacaaacaca gagctattcg atatgtgggg ggttcttatc agtgagggat ggctctaaca
       61 agactcctgc tccctatttc tatcctttgg ttttgttttt attcctctca caccatattg
      121 caaaaggacc cactccttat atgtgtaaat ggagaccctg gctttgacca aagggcctac
      181 cccacctatt ttggtcccat tttagatgag ttctctagca tcatgggatt tgagccctcc
      241 atcttgagtt tggagcggtt taatccagtg ggtggtccgg aaacttcacc agatacagat
      301 ataagtgtgg atgattttgg agccagaggt gatgggacag atgacacaaa ggcatttgag
      361 aaggcttgga aggacgcttg ttcatcagga tctgtcctca ttgtgcctga aaacaagaac
      421 tatcttctta aacaaattac cttttcaggt ccttgtaaat ctgatcttcg tgtgaagatt
      481 cgcggaacga ttgaagcttc atctgatcaa tcagattggg tgggacataa tcgaaaacga
      541 tggattgaat ttgaagacat cagcaacctc acactagaag gcggtggaac atccaatgga
      601 aatggagaga catggtggga tagctcctgc aaaaggaaga aatcccttcc ttgcaaaagc
      661 gcacctacgg ctctaacctt caggtcctgc aagaacctga ttgtgagcga tctcagtatc
      721 aaggacagcc aaaagatgca tctttctttt gataagtgcc aagatgtcat agcttctaat
      781 ctcatggtaa ctgcaccaga gcatagcccc aacacagatg gcattcatat aacaggcacc
      841 caaagaatcc atgtgatgaa ttctgtcatc ggaacaggtg atgattgtat ctcaatagag
      901 agtggatcaa aaatggtgat agccacaaac ataacctgtg gaccaggcca tggaattagc
      961 attggaagct taggagatcg aaattcagaa gctcatgttt caggagtact tgtggatggc
     1021 ggcaaccttt tcgatacaac aaatggactc aggatcaaaa catggcaggg aggttcagga
     1081 agtgcaaaga acatcaaatt ccaaaacatt gtcatgcaca acgtcacaaa tcctataatc
     1141 atagatcaat actactgtga ctccaaggac ccatgtccgg aacaggaatc agctgtgaag
     1201 gtaagcaatg ttgcctacat gaacatccgt ggaacaagtg cctctgaggt ggctgtaaaa
     1261 ttcgattgca gcaagagctc tccatgccaa gggtatattg ttggaaacat caatctggtt
     1321 gggaatggag ggaaggaaac aaccatgtca tgcagcaata ttgttcaagg actactgagg
     1381 gaaggtctat ccaccttctt gtttatgaaa agaagggttc atgaatgtag ttattgatat
     1441 taagtgtcat tacatgaagg aaaaagtgac atgggccata gttacttcaa ccatgctata
     1501 gggtggtcag ttcagataac tagttttcag tatctctctg atgtacttga tgtagttcat
     1561 atatatttat atatatatat atatatatat atcaatgact agaaagtcaa ataaggaaga
     1621 ttgtttccat cagtcgggta aggaaaatgg ttggaaaatg tggccatgaa ttgtaacata
     1681 attcctgtta ttcatgttaa atcatgcctg attcaaataa taatcaatta ccaattgagt
     1741 gtgggtttct cattc
//