From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: type D neurotoxin [bacteriophage d-16 phi, host = C. botulinum,
Date: 26 Jan 93 05:50:46 GMT
Distribution: bionet
Checksum: 42335 9

LOCUS       S118639      4087 bp    DNA             PHG       25-JAN-1993
DEFINITION  type D neurotoxin [bacteriophage d-16 phi, host = C. botulinum,
            type D, CB16, Genomic, 4087 nt]
ACCESSION   S49407
KEYWORDS    .
SOURCE      bacteriophage d-16 phi CB16 type D host = C. botulinum
  ORGANISM  Unclassified.
            Unclassified.
REFERENCE   1  (bases 1 to 4087)
  AUTHORS   Sunagawa,H., Ohyama,T., Watanabe,T. and Inoue,K.
  TITLE     The complete amino acid sequence of the Clostridium botulinum type
            D neurotoxin, deduced by nucleotide sequence analysis of the
            encoding phage d-16 phi genome.
  JOURNAL   J Vet Med Sci 54, 905-913 (1992)
  STANDARD  full automatic
COMMENT     This entry [NCBI gibbsq 118639] was created by the journal scanning
            component of NCBI/GenBank at the National Library of Medicine.
            This sequence comes from Fig. 4.  The authors begin their numbering
            at -181.  Zero is not present in the numbering.
FEATURES             Location/Qualifiers
     CDS             182..4012
                     /note="For the protein sequence (NCBI gibbsq 118641):
                     Method: conceptual translation with partial peptide
                     sequencing.  This sequence comes from Fig. 4."
                     /product="type D neurotoxin"
                     /codon_start=1
                     /translation="MTWPVKDFNYSDPVNDNDILYLRIPQNKLITTPVKAFMITQNIW
                     VIPERFSSDTNPSLSKPPRPTSKYQSYYDPSYLSTDEQKDTFLKGIIKLFKRINERDI
                     GKKLINYLVVGSPFMGDSSTPEDTFDFTRHTTNIAVEKFENGSWKVTNIITPSVLIFG
                     PLPNILDYTASLTLQGQQSNPSFEGFGTLSILKVAPEFLLTFSDVTSNQSSAVLGKSI
                     FCMDPVIALMHELTHSLHQLYGINIPSDKRIRPQVSEGFFSQDGPNVQFEELYTFGGL
                     DVEIIPQIERSQLREKALGHYKDIAKRLNNINKTIPSSWISNIDKYKKIFSEKYNFDK
                     DNTGNFVVNIDKFNSLYSDLTNVMSEVVYSSQYNVKNRTHYFSRHYLPVFANILDDNI
                     YTIRDGFNLTNKGFNIENSGQNIERNPALQKLSSESVVDLFTKVCLRLTKNSRDDSTC
                     IKVKNNRLPYVADKDSISQEIFENKIITDETNVQNYSDNFSLDESILDGQVPINPEIV
                     DPLLPNVNMEPLNLPGEEIVFYDDITKYVDYLNSYYYLESQKLSNNVENITLTTSVEE
                     ALGYSNKIYTFLPSLAEKVNKGVQAGLFLNWANEVVEDFTTNIMKKDTLDKISDVSVI
                     IPYIGPALNIGNSALRGNFKQAFATAGVAFLLEGFPEFTIPALGVFTFYSSIQEREKI
                     IKTIENCLEQRVKRWKDSYQWMVSNWLSRITTQFNHINYQMYDSLSYQADAIKAKIDL
                     EYKKYSGSDKENIKSQVENLKNSLDVKISEAMNNINKFIRECSVTYLFKNMLPKVIDE
                     LNKFDLRTKTELINLIDSHNIILVGEVDRLKAKVNESFENTMPFNIFSYTNNSLLKDI
                     INEYFNSINDSKILSLQNKKNALVDTSGYNAEVRVGDNVQLNTIYTNDFKLSSSGDKI
                     IVNLNNNILYSAIYENSSVSFWIKISKDLTNSHNEYTIINSIEQNSGWKLCIRNGNIE
                     WILQDVNRKYKSLIFDYSESLSHTGYTNKWFFVTITNNIMGYMKLYINGELKQSQKIE
                     DLDEVKLDKTIVFGIDENIDENQMLWIRDFNIFSKELSNEDINIVYEGQILRNVIKDY
                     WGNPLKFDTEYYIINDNYIDRYIAPESNVLVLVRYPDRSKLYTGNPITIKSVSDKNPY
                     SRILNGDNIILHMLYNSRKYMIIRDTDTIYATQGGECSQNCVYALKLQSNLGNYGIGI
                     FSIKNIVSKNKYCSQIFSSFRENTMLLADIYKPWRFSFKNAYTPVAVTNYETKLLSTS
                     SFWKFISRDPGWVE"
BASE COUNT     1646 a    413 c    633 g   1395 t
ORIGIN      
        1 tatgcaaaag attacagtta atatctgatt attagacctg atgataacat tttcatataa
       61 tgataaatat ttttctctat cacttagaga tggagatata attggatgat atgcaatgac
      121 aataacaagg tgctaaaggt gcacacttgt ggatattaga aagttaggag atgttagtat
      181 tatgacatgg ccagtaaaag attttaatta tagtgatcct gttaatgaca atgatatatt
      241 atatttaaga ataccacaaa ataagttaat tactacacct gtaaaagctt ttatgattac
      301 tcaaaatatt tgggtaatac cagaaagatt ttcatcagat actaatccaa gtttaagtaa
      361 accgcccaga cctacttcaa agtatcaaag ttattatgat cctagttatt tatctactga
      421 tgaacaaaaa gatacatttt taaaagggat tataaaatta tttaaaagaa ttaatgaaag
      481 agatatagga aaaaaattaa taaattattt agtagttggt tcacctttta tgggagattc
      541 aagtacgcct gaagatacat ttgattttac acgtcatact actaatattg cagttgaaaa
      601 gtttgaaaat ggtagttgga aagtaacaaa tattataaca ccaagtgtat tgatatttgg
      661 accacttcct aatatattag actatacagc atcccttaca ttgcaaggac aacaatcaaa
      721 tccatcattt gaagggtttg gaacattatc tatactaaaa gtagcacctg aatttttgtt
      781 aacatttagt gatgtaacat ctaatcaaag ttcagctgta ttaggcaaat ctatattttg
      841 tatggatcca gtaatagctt taatgcatga gttaacacat tctttgcatc aattatatgg
      901 aataaatata ccatctgata aaaggattcg tccacaagtt agcgagggat ttttctctca
      961 agatggaccc aacgtacaat ttgaggaatt atatacattt ggaggattag atgttgaaat
     1021 aatacctcaa attgaaagat cacaattaag agaaaaagca ttaggtcact ataaagatat
     1081 agcgaaaaga cttaataata ttaataaaac tattccttct agttggatta gtaatataga
     1141 taaatataaa aaaatatttt ctgaaaagta taattttgat aaagataata caggaaattt
     1201 tgttgtaaat attgataaat tcaatagctt atattcagac ttgactaatg ttatgtcaga
     1261 agttgtttat tcttcgcaat ataatgttaa aaacaggact cattattttt caaggcatta
     1321 tctacctgta tttgcaaata tattagatga taatatttat actataagag atggttttaa
     1381 tttaacaaat aaaggtttta atatagaaaa ttcgggtcag aatatagaaa ggaatcctgc
     1441 actacaaaag cttagttcag aaagtgtagt agatttattt acaaaagtat gtttaagatt
     1501 aacaaaaaat agtagagatg attcaacatg tattaaagtt aaaaataata gattacctta
     1561 tgtagctgat aaagatagca tttcacaaga aatatttgaa aataaaatta ttacagatga
     1621 gactaatgta caaaattatt cagataattt ttcattagat gaatctattt tagatgggca
     1681 agttcctatt aatcctgaaa tagtagatcc actattaccc aatgttaata tggaaccttt
     1741 aaatcttcca ggtgaagaaa tagtatttta tgatgatatt actaaatatg ttgattattt
     1801 aaattcttat tattatttgg aatctcaaaa attaagtaat aatgttgaaa atattactct
     1861 tacaacttca gttgaagaag cattaggtta tagcaataag atatacacat ttttacctag
     1921 cttagctgaa aaagtgaata aaggtgttca agcaggttta ttcttaaatt gggcgaatga
     1981 agtagttgag gattttacta caaatattat gaagaaagat acattggata aaatatcaga
     2041 tgtatcagta ataattccat atataggacc tgccttaaat ataggaaatt cagcattaag
     2101 gggaaatttt aagcaagcat ttgcaacagc tggtgtagct tttttattag agggatttcc
     2161 agagtttact atacctgcac tcggtgtatt taccttttat agttctattc aagaaagaga
     2221 gaaaattatt aaaactatag aaaattgttt ggaacaaaga gttaagagat ggaaagattc
     2281 atatcaatgg atggtatcaa attggttgtc aagaattact actcaattta atcatataaa
     2341 ttatcaaatg tatgattctt taagttatca ggcagatgca atcaaagcta aaatagattt
     2401 agaatataaa aaatactcag gaagtgataa agaaaatata aaaagtcaag ttgaaaattt
     2461 aaaaaatagt ttagatgtaa aaatttcgga agcaatgaat aatataaata aatttatacg
     2521 agaatgttct gtaacatact tatttaaaaa tatgctccct aaagtaattg acgaattaaa
     2581 taagtttgat ttaagaacta aaacagaatt aattaatctt atagatagtc ataatattat
     2641 tctagttggt gaagtagata gattaaaagc aaaagtaaat gagagttttg aaaatacaat
     2701 gccttttaat attttttcat atactaataa ttctttatta aaagatataa ttaatgaata
     2761 tttcaatagt attaatgatt caaaaatttt gagcttacaa aacaaaaaaa atgctttagt
     2821 ggatacatca ggatataatg cagaagtgag ggtaggagat aatgttcaac ttaatacgat
     2881 atatacaaat gactttaaat taagtagttc aggagataaa attatagtaa atttaaataa
     2941 taatatttta tatagcgcta tttatgagaa ctctagtgtt agtttttgga ttaagatatc
     3001 taaagattta actaattctc ataatgaata tacaataatt aacagtatag aacaaaattc
     3061 tgggtggaaa ttatgtatta ggaatggcaa tatagaatgg attttacaag atgttaatag
     3121 aaagtataaa agtttaattt ttgattatag tgaatcatta agtcatacag gatatacaaa
     3181 taaatggttt tttgttacta taactaataa tataatgggg tatatgaaac tttatataaa
     3241 tggagaatta aagcagagtc aaaaaattga agatttagat gaggttaagt tagataaaac
     3301 catagtattt ggaatagatg agaatataga tgagaatcag atgctttgga ttagagattt
     3361 taatattttt tctaaagaat taagtaatga agatattaat attgtatatg agggacaaat
     3421 attaagaaat gttattaaag attattgggg aaatcctttg aagtttgata cagaatatta
     3481 tattattaat gataattata tagataggta tattgcacct gaaagtaatg tacttgtact
     3541 tgttcggtat ccagatagat ctaaattata tactggaaat cctattacta ttaaatcagt
     3601 atctgataag aatccttata gtagaatttt aaatggagat aatataattc ttcatatgtt
     3661 atataatagt aggaaatata tgataataag agatactgat acaatatatg caacacaagg
     3721 aggagagtgt tcacaaaatt gtgtatatgc attaaaatta cagagtaatt taggtaatta
     3781 tggtataggt atatttagta taaaaaatat tgtatctaaa aataaatatt gtagtcaaat
     3841 tttctctagt tttagggaaa atacaatgct tctagcagat atatataaac cttggagatt
     3901 ttcttttaaa aatgcataca cgccagttgc agtaactaat tatgaaacaa aactattatc
     3961 aacttcatct ttttggaaat ttatttctag ggatccagga tgggtagagt aatacaataa
     4021 aaatttaata taaactatta aattatatta caagttttag aaatttatcg tataaaatgt
     4081 tgaattc
//