From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Murine cytomegalovirus glycoprotein B (gB) gene, complete cds.
Date: 18 Aug 92 10:02:21 GMT
Distribution: bionet
Checksum: 15274     7

LOCUS       HS5GLYCPRB   3280 bp ds-DNA             VRL       18-AUG-1992
DEFINITION  Murine cytomegalovirus glycoprotein B (gB) gene, complete cds.
ACCESSION   M86302
KEYWORDS    envelope glycoprotein; glycoprotein B.
SOURCE      Murine cytomegalovirus DNA.
  ORGANISM  Murine cytomegalovirus
            Viridae; ds-DNA enveloped viruses; Herpesviridae;
            Betaherpesvirinae.
REFERENCE   1  (bases 1 to 3280)
  AUTHORS   Rapp,M., Messerle,M., Buehler,B., Tannheimer,M., Keil,G.M. and
            Koszinowski,U.H.
  TITLE     Identification of the murine cytomegalovirus glycoprotein B gene
            and its expression by recombinant vaccinia virus
  JOURNAL   J. Virol. 66, 4399-4406 (1992)
  STANDARD  full staff_entry
FEATURES             Location/Qualifiers
     TATA_signal     10..14
                     /gene="gB"
     mRNA            37..3190
                     /note="alternative transcription start"
                     /gene="gB"
                     /evidence=EXPERIMENTAL
     TATA_signal     83..88
                     /gene="gB"
     mRNA            117..3190
                     /note="alternative transcription start"
                     /gene="gB"
                     /evidence=EXPERIMENTAL
     CDS             287..3073
                     /product="glycoprotein B"
                     /gene="gB"
                     /codon_start=1
                     /translation="MSRRNERGCRSSSWYAMSTALAVTIWCLLACTSEVIAAASTPGT
                     TPKAKTDTSSETASAETETATSGAATGKKEATPTQASKITGTTIVPFVNETEDMVSVD
                     IDKYPYRVCMAVSTDLVRFGKSIDCINHTPKTPVQEGIMVVYKENIVAYTFEVITYHK
                     DAIFQRSYADTTTNYFLGTSVTKMAFPIWELDEVNRNNRCYSAASRILNGEVYVAYHE
                     DSYRNYTMVLVEDDYRSKNSKRYVTTKSRYHKGAWTWRYTESCNMNCVVVVTKARSNT
                     PYEFFVLSSGEVVEISPFYNGENSEPFEEDTRNFWIRKNYTMKTYFGELAAPKKVVPL
                     MAFLEREDMTIGWEIFPKQNVTCDWKKWQTVSRAIRTDTNTGYHFVSKGLTATFVASK
                     RKIDYNTTTEGKNYNTFRCVYDEFVEEVNRVFEDEYNETHVKDGELEMYRTTGGLIVL
                     WQGLKAKSLHNLEKFAALNNVSVATASPPVTTAATENGTTVRSRRKRSFDNLDDVVTD
                     ISYAQLQFTYDVLKDYINDALRNIMDAWCRDQKRTAEMLKELSKINPSNILSAIYERP
                     VTAKLAGDVIAMSECVKVDQSSVKVLKDMRIFQDGKVVNCYSRPLVVFQFINSTKLES
                     GQLGENNEIMLGTFRTENCDTNSRKIFVVGTVGYEYRDYRFRNVTSLEHIDLVDTLIG
                     LDIEPLENTDFKVLELYSKGELRASNVFSLDEIMREYNSQKQHIRTLSAKVNDNTPSY
                     LLGLDTFMQGLGVAGKGIGVAIGAVGGAVSSVVNAVTGFLTNPFGGFTTILLVIGVLA
                     VVYLIFTRPVEYFFPYATQTAVQYAPPGGAHGGLESGPPGAPGLHRRVNAGGSDDSGK
                     AWTSDKKGLERTYTEQDALLILRALKQLDDSQRNEKAQQKATRLPTGILDRLKGNDTS
                     GYQRLPAEDSDFEY"
     polyA_signal    3162..3167
                     /gene="gB"
     polyA_signal    3222..3227
                     /gene="gB"
BASE COUNT      838 a    809 c    938 g    695 t
ORIGIN      Map position 0.361 to 0.377 of the genome
        1 gtcgcactat ataacgagac gttcggcaag cagttggcct tgtggcgcgt cgaggacggc
       61 gatgagatcg gcgacgggat aatattaacg tacaatccag aatctccgtt gatcctgcgt
      121 cgcggagatc gaagttacag atccagggat ctgtacttgc tgctctacaa gcatctctcg
      181 gtcgactccg agactctcgc cgacgccggt tctcgcgcct cggtcgccga cttatgtcag
      241 gtcgagagac ccgggccgat cgctgaacaa cgctcgtcga cgcagaatgt caagaagaaa
      301 cgaaagagga tgtcgctcct cgagctggta cgcgatgtcg acggcgctgg cggtgacgat
      361 ctggtgcctc cttgcctgta caagtgaggt aatcgcggcg gcgagtacac caggtactac
      421 tccgaaagca aaaactgaca cttcgtcaga aaccgcgtcc gcagaaacag agacggcgac
      481 ttcgggcgcg gcgaccggaa agaaggaagc cacgccgact caggcctcga agataacggg
      541 gaccaccatc gttcccttcg taaacgagac ggaggacatg gtttctgttg acatagataa
      601 atatccttac agagtgtgca tggctgtgtc tactgatctc gttcggttcg ggaaaagtat
      661 agattgtatc aatcataccc cgaagacacc cgtacaagag gggatcatgg tagtttacaa
      721 agaaaatatc gtggcttaca cgtttgaggt gatcacttat cacaaagatg cgatctttca
      781 gagaagttat gctgacacga ctactaatta tttcttgggg actagcgtga ctaagatggc
      841 cttccccatc tgggagctgg atgaggtaaa cagaaacaac aggtgttact ctgccgcgag
      901 taggatactc aatggggaag tgtatgtcgc ttaccacgaa gatagctata ggaactatac
      961 tatggtgctt gtggaggatg attacaggag taagaacagt aagcgatacg tgaccaccaa
     1021 gagtaggtat cataagggcg cttggacgtg gagatacacc gaatcctgta acatgaactg
     1081 cgtcgtcgtc gtcacgaaag ctaggtctaa caccccctac gaattcttcg tgctcagctc
     1141 cggggaagtc gtcgagatat caccgtttta caacggtgaa aactccgagc ccttcgaaga
     1201 ggacacgaga aatttctgga ttcgcaagaa ttacaccatg aagacgtact tcggtgaact
     1261 cgcggcaccc aaaaaagtag tacctctgat ggcttttcta gagagggagg acatgaccat
     1321 cgggtgggag atctttccca aacagaatgt cacgtgcgac tggaagaaat ggcagacggt
     1381 ttcgagggcg atacgcactg acaccaatac tggctatcat tttgtttcca agggtttaac
     1441 tgctactttt gtggccagta aacgcaaaat agattataat actactaccg aaggtaaaaa
     1501 ttataacacc ttccgatgtg tttatgatga gtttgtagaa gaagtgaatc gtgtgtttga
     1561 agatgagtac aatgagacgc atgtgaaaga cggggagctt gaaatgtata gaacaacggg
     1621 aggtcttatt gtattgtggc agggattgaa agcgaaatca ctgcataatc tggaaaaatt
     1681 tgccgcgttg aataacgttt cagtggcgac tgctagccct cctgtaacca ctgccgcgac
     1741 tgagaatggt actactgttc gttctcgccg aaaacgcagc tttgacaacc tagatgatgt
     1801 ggtgacggat atcagctatg ctcaactgca atttacctac gacgtgctca aggactacat
     1861 caacgatgcg ctccgaaaca tcatggacgc ttggtgtcgc gaccagaaac ggacggcgga
     1921 gatgctcaag gagctcagta agatcaatcc gtccaacatc ttgtcggcca tctacgagag
     1981 accggtcacc gccaagctgg cgggcgacgt catcgcgatg tccgagtgtg tcaaggtcga
     2041 tcagagtagt gtgaaggtcc tgaaggacat gcggatcttc caggacggga aggtcgtcaa
     2101 ctgttattcg agaccgctgg tcgtctttca gttcatcaac tcgacgaagc tcgagtcggg
     2161 ccagctgggc gagaacaacg agatcatgct gggcaccttt cggacggaga actgcgacac
     2221 gaacagcagg aagatcttcg tcgtggggac tgtgggctac gagtatcggg actaccgttt
     2281 caggaacgtg accagtctgg agcacatcga cctcgtcgac accctgatcg ggctggacat
     2341 cgagcccctg gagaacaccg acttcaaggt gctcgagctg tactcgaagg gagagctgcg
     2401 cgcctcgaac gtgttcagcc tggacgagat catgcgggag tacaactcgc agaagcagca
     2461 catccgcacc ctgagcgcca aagtcaacga caacacgccc tcttatctct tggggttgga
     2521 tactttcatg caggggctag gggtcgcggg taaggggatc ggggtggcca tcggggccgt
     2581 cggcggggcc gtgtcttcgg tggtgaacgc cgtcacgggc ttcctgacga acccgtttgg
     2641 aggtttcacc accatcctcc tggtgatcgg cgtgctagcc gtcgtgtatc tcatcttcac
     2701 gaggccggtc gagtacttct tcccctacgc cacccagacc gccgtgcagt atgctcctcc
     2761 cggcggcgcg cacgggggac tcgagagcgg gccgccggga gcgcctggtc tgcatcggcg
     2821 cgtgaacgcg ggcggcagtg acgattcggg taaggcgtgg actagcgata aaaagggctt
     2881 ggagaggacc tacaccgagc aagatgctct attgatactc cgcgcgttaa aacagctaga
     2941 cgacagccaa cgcaacgaga aagcgcagca gaaagcgact agactgccga cgggcatctt
     3001 ggaccgtctc aaaggtaacg acaccagtgg ctaccagcga ttaccggcgg aggactccga
     3061 tttcgagtac tgacggccga catggacact tgcgtggaga ctttcttcaa cccctacctg
     3121 cgacggaaac cccgtcgcga ttggagacgc tgcgaggata ataataaaaa ctttttacaa
     3181 gtcgttccgc gtggcgtgtt gtatgatggt gcgacggggt taataaaggt acagtccggc
     3241 atggaaccca ggatgttcta cgccgagaaa gagtacgtgc
//