From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Puumala virus K27 polyprotein encoding glycoproteins G1 and G2,
Date: 8 Jan 93 05:49:45 GMT
Distribution: bionet
Checksum: 16753 8

LOCUS       PUUMG12A     3456 bp ss-RNA             VRL       07-JAN-1993
DEFINITION  Puumala virus K27 polyprotein encoding glycoproteins G1 and G2,
            complete cds.
ACCESSION   L08754
KEYWORDS    glycoprotein G1; glycoprotein G2; polyprotein.
SOURCE      Puumala virus (strain K27) cDNA to genomic RNA.
  ORGANISM  Puumala virus
            Viridae; ss-RNA enveloped viruses; Negative strand RNA viruses;
            Bunyaviridae; Hantavirus.
REFERENCE   1  (bases 1 to 3456)
  AUTHORS   Xiao,S.-Y., Spik,K. and Schmaljohn,C.S.
  TITLE     Nucleotide sequence of the M and S genome segments of Puumala virus
            K27
  JOURNAL   Unpublished (1993)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     misc_feature    1..3456
                     /note="This region was PCR amplified and is corresponding
                     to nt 39 to3439 of the putative full-length M genome
                     segment"
     CDS             3..3449
                     /note="glycoproteins G1 and G2"
                     /product="glycoprotein"
                     /codon_start=1
                     /translation="MGELSPVCLYLLLQGLLLCNTGAARNLNELKMECPHTIRLGQGL
                     VVGSVELPSLPIQQVETLKLESSCNFDLHTSTAGQQSFTKWTWEIKGDLAENTQASST
                     SFQTKSSEVNLRGLCLIPTLVVETAARMRKTIACYDLSCNQTVCQPTVYLMGPIQTCI
                     TTKSCLLSLGDQRIQVNYEKTYCVSGQLVEGICFNPIHTMALSQPSHTYDIMTMMVRC
                     FLVIKKVTSGDSMKIEKNFETLVQKNGCTANNFQGYYICLIGSSSEPLYVPALDDYRS
                     AEVLSRMAFAPHGEDHDIEKNAVSAMRIAGKVTGKAPSTESSDTVQGIAFSGSPLYTS
                     TGVLTSKDDPVYIWAPGIIMEGNHSICEKKTLPLTWTGFISLPGEIEKTTQCTVFCTL
                     AGPGADCEAYSETGIFNISSPTCSINRVQRFRGSEQQIKFVCQRVDMDITVYCNGMKK
                     VILTKTLVIGQCIYTFTSIFSLIPGVAHSLAVELCVPGLHGWATMLLLLTLCFGWVLI
                     PTITMILLKILIAFAYLCSKYNTDSKFRILIEKVKREYQKTMGSMVCEVCQYECETAK
                     ELESHRKSCSIGSCPYCLNPSEATTSALQAHFKVCKLTSRFQENLRKSLTVYEPMQGC
                     YRTLSLFRYRSRFFVGLVWCVLLVLELIVWAASAETQNLNAGWTDTAHGSGIIPMKTD
                     LELDFSLPSSASYTYRRQLQNPANEQEKIPFHLQLSKQVIHAEIQHLGHWMDATFNLK
                     TAFHCYGSCEKYAYPWQTAGCFIEKDYEYETGWGCNPPDCPGVGTGCTACGVYLDKLK
                     SVGKVFKIVSLRYTRKVCIQLGTEQTCKTVDSNDCLITTSVKVCLIGTISKFQPSDTL
                     LFLGPLQQGGLIFKQWCTTTCQFGDPGDIMSTPTGMKCPELNGSFRKKCAFATTPVCQ
                     FDGNTISGYKRMIATKDSFQSFNVTEPHISTSALEWIDPDSSLRDHINVIVSRDLSFQ
                     DLSETPCQIDLATASIDGAWGSGVGFNLVCTVSLTECSAFLTSIKACDAAMCYGSTTA
                     NLVRGQNTIHIVGKGGHSGSKFMCCHDTKCSSTGLVAAAPHLDRVTGYNQADSDKIFD
                     DGAPECGMSCWFKKSGEWILGVLNGNWMVVAVLVVLLILSILLFTLCCPRRPSYRKEH
                     KP"
BASE COUNT     1047 a    625 c    751 g   1033 t
ORIGIN      
        1 atatgggaga acttagtcca gtttgtctgt atctgcttct ccagggtcta ttactatgta
       61 atacaggggc tgccagaaac cttaatgagc ttaaaatgga atgtccacat actattagat
      121 tagggcaggg tcttgttgtg ggttcagtag aattgccatc tcttccaata cagcaggtcg
      181 agacactaaa gctggagagt tcttgtaatt ttgatctaca taccagtaca gcaggacaac
      241 aatcattcac aaaatggaca tgggaaatta aaggtgatct tgcagagaac acacaggcat
      301 catcaacaag ttttcaaaca aaaagcagtg aagtgaattt gagaggatta tgtttgatcc
      361 ctactttagt ggttgaaaca gcagcaagaa tgcgaaaaac aatagcatgt tatgacctgt
      421 catgcaatca aacagtgtgt cagcctactg tctatttaat gggacctatc cagacttgta
      481 taacaactaa aagttgtctc ctgagtttag gtgatcaaag gattcaagta aattatgaaa
      541 aaacatactg tgtttctggg cagcttgttg aaggtatctg ttttaatcca atacatacaa
      601 tggcactctc tcaacctagt catacatatg atataatgac catgatggtt cgctgtttct
      661 tggtaataaa gaaagtgact tctggtgaca gtatgaagat tgaaaagaac tttgagactc
      721 ttgttcaaaa aaatggctgc acagctaata acttccaagg gtattatatc tgtcttatag
      781 ggagtagttc agagccccta tatgttccag cattagatga ttatcgttca gctgaagttc
      841 tttcaaggat ggcatttgca ccacatggtg aagatcatga tattgagaaa aatgcagtga
      901 gtgcaatgcg tattgctgga aaggtgacag gaaaggcgcc atcaacagaa tcatcagata
      961 cagtacaggg gattgcattt tcaggtagtc ctctttatac atctactggt gtcttgacat
     1021 caaaagatga tcctgtctac atttgggctc ctggaatcat aatggaagga aaccattcta
     1081 tttgtgaaaa gaagacctta ccccttacat ggactggttt tatttcattg cctggagaga
     1141 ttgaaaaaac aacacaatgt acagtatttt gtacattggc tggaccaggt gcagattgtg
     1201 aagcttactc tgaaacaggc atcttcaaca taagttcacc tacttgctca ataaatcgtg
     1261 tccagagatt ccgtggttca gaacagcaaa taaagtttgt gtgccagaga gtggacatgg
     1321 atatcactgt ttactgtaat gggatgaaga aagtcattct caccaagacc ctagttattg
     1381 gacaatgcat ttatactttt actagtattt tctctctaat ccctggtgtt gcacattccc
     1441 ttgctgttga attatgtgta cctggtcttc atggttgggc aactatgcta ttattactaa
     1501 cactttgttt tggctgggtc ttaataccaa ctataacaat gatcctgcta aagatattga
     1561 ttgcattcgc atacttatgt tctaaatata acacagattc gaaattcagg atcttgattg
     1621 agaaagtgaa aagagagtac cagaaaacaa tgggttcaat ggtttgtgaa gtgtgtcagt
     1681 atgaatgtga gactgcaaaa gaactggagt cacatagaaa gagttgttcc attggttcat
     1741 gcccttattg tctcaatcca tctgaggcaa caacatctgc ccttcaggct cattttaaag
     1801 tgtgtaagct cacatcacgg tttcaggaga atttaagaaa gtcattaacg gtatatgagc
     1861 ctatgcaagg gtgctaccgg actttatccc tctttagata taggagtcgg ttctttgtgg
     1921 gtctagtctg gtgcgtgtta ttggttctag agttaattgt atgggctgcc agtgctgaaa
     1981 cacaaaattt aaatgcaggt tggacagaca cagcacatgg atctggaatt atacctatga
     2041 aaactgatct ggaattagac ttctctcttc cgtcatcagc aagctataca tataggagac
     2101 agctacaaaa cccagcaaac gaacaagaga aaatcccatt tcatctgcag ttaagcaaac
     2161 aagtgattca tgcagagatc cagcatttag gtcattggat ggatgctaca tttaatctta
     2221 aaactgcatt tcactgctat ggctcatgtg agaagtatgc ttatccttgg cagacagcag
     2281 gttgtttcat agaaaaagat tatgaatatg agactggttg gggttgtaat ccacctgatt
     2341 gcccaggggt agggacaggc tgtactgctt gtggggtata ccttgataaa ttaaaatcag
     2401 ttggaaaggt tttcaaaatt gtgtccttaa gatacacaag gaaagtatgc attcagttgg
     2461 gcacagaaca aacatgtaag actgttgata gtaatgactg tctcattacc acttcagtta
     2521 aagtgtgctt gatagggacc atatcaaaat tccaaccatc tgacactttg ctatttctag
     2581 gtccactaca gcagggtggt ctgatattta aacaatggtg cactacaaca tgccagtttg
     2641 gcgatcccgg ggacataatg agcacaccta caggcatgaa gtgcccagaa ttaaatggtt
     2701 cttttagaaa gaaatgtgca tttgcaacaa ctccagtttg ccagtttgat ggaaatacaa
     2761 tttcaggcta taagaggatg attgccacaa aggattcatt tcaatctttc aatgtgacag
     2821 aaccccatat ttctacaagt gcacttgaat ggattgatcc tgacagctca cttagggacc
     2881 atattaatgt aattgtgagt cgtgatctat ccttccaaga cctaagtgaa acaccatgtc
     2941 aaattgattt agcaacagcc tctatagatg gagcatgggg ttcaggagtt ggttttaatc
     3001 tggtttgtac tgttagttta acagaatgtt ctgcatttct gacatcaatc aaggcctgtg
     3061 atgctgcaat gtgttatggg tccaccacag ccaatctagt tcgagggcaa aataccattc
     3121 atatcgtcgg taagggtggg cattctggtt caaaatttat gtgttgtcat gacacaaaat
     3181 gttctagcac cggtctagtt gcagctgcac cacacttaga tcgtgtgaca ggatacaatc
     3241 aggctgatag tgacaaaatc tttgatgatg gggcaccaga atgtggtatg tcatgttggt
     3301 ttaaaaaatc aggtgaatgg attcttgggg ttttgaacgg gaattggatg gttgttgctg
     3361 tactggtagt attactgatc ttgtccatac tcttattcac attatgttgt cctcgtagac
     3421 ctagttacag gaaagaacat aagccctaag ttttgc
//