From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: L.lactis genes for nisin and biosynthetic enzymes
Date: 3 Jan 93 22:16:09 GMT
Distribution: bionet
Checksum: 48266 15

LOCUS       LACNISI      7500 bp ds-DNA             BCT       23-DEC-1992
DEFINITION  L.lactis genes for nisin and biosynthetic enzymes
ACCESSION   X68307
KEYWORDS    biosynthetic enzyme; lantibiotic; nisin.
SOURCE      Lactococcus lactis DNA.
  ORGANISM  Lactococcus lactis
            Prokaryotae; Firmicutes; Firmibacteria; Gram-positive cocci;
            Streptococcaceae.
REFERENCE   1  (bases 1 to 7500)
  AUTHORS   Engelke,G., Gutowski-Eckel,Z., Hammelmann,M. and Entian,K.-.
  TITLE     Biosynthesis of the Lantibiotic Nisin: Genomic Organization and
            Membrane Localization of the NisB Protein
  JOURNAL   Appl. Environ. Microbiol. 58, 3730-3743 (1992)
  STANDARD  full automatic
COMMENT     *source: strain=6F3; *source: clone=pGEN1, pGEN2, pGEN3;
               From EMBL    entry LLNISI;  dated 09-DEC-1992.
FEATURES             Location/Qualifiers
     CDS             1..329
                     /partial
                     /gene="nisX"
                     /partial
                     /product="E.coli-like transposase"
                     /codon_start=3
                     /translation="ACGKEHPQPGLIVHTDQGSQYTSSRYQSTLRQVGAQSSMSRKGN
                     PYDNAMMESFYKTLKRELINDAHFETRAEATQEIFKYIETYYNTKRMHSGLDYKSPKD
                     FEKYNS"
     CDS             1159..1332
                     /gene="nisA"
                     /product="Nisin-prepeptide"
                     /codon_start=1
                     /translation="MSTKDFNLDLVSVSKKDSGASPRITSISLCTPGCKTGALMGCNM
                     KTATCHCSIHVSK"
     CDS             1439..4420
                     /gene="nisB"
                     /note="biosynthetic gene"
                     /codon_start=1
                     /translation="MIKSSFKAQPFLVRNTILCPNDKRSFTEYTQVIETVSKNKVFLE
                     QLLLANPKLYDVMQKYNAGLLKKKRVKKLFESIYKYYKRSYLRSTPFGLFSETSIGVF
                     SKSSQYKLMGKTTKGIRLDTQWLIRLVHKMEVDFSKKLSFTRNNANYKFGDRVFQVYT
                     INSSELEEVNIKYTNVYQIISEFCENDYQKYEDICETVTLCYGDEYRELSEQYLGSLI
                     VNHYLISNLQKDLLSDFSWNTFLTKVEAIDEDKKYIIPLKKVQKFIQEYSEIEIGEGI
                     EKLKEIYQEMSQILENDNYIQIDLISDSEINFDVKQKQQLEHLAEFLGNTTKSVRRTY
                     LDDYKDKFIEKYGVDQEVQITELFDSTFGIGAPYNYNHPRNDFYESEPSTLYYSEEER
                     EKYLSMYVEAVKNHNVINLDDLESHYQKMDLEKKSELQGLELFLNLAKEYEKDIFILG
                     DIVGNNNLGGASGRFSALSPELTSYHRTIVDSVERENENKEITSCEIVFLPENIRHAN
                     VMHTSIMRRKVLPFFTSTSHNEVLLTNIYIGIDEKEKFYARDISTQEVLKFYITSMYN
                     KTLFSNELRFLYEISLDDKFGNLPWELIYRDFDYIPRLVFDEIVISPAKWKIWGRDVN
                     SKMTIRELIQSKEIPKEFYIVNGDNKVYLSQKNPLDMEILESAIKKSSKRKDFIELQE
                     YFEDENIINKGEKGRVADVVVPFIRTRALGNEGRAFIREKRVSVERREKLPFNEWLYL
                     KLYISINRQNEFLLSYLPDIQKIVANLGGNLFFLRYTDPKPHIRLRIKCSDLFLAYGS
                     ILEILKRSRKNRIMSTFDISIYDQEVERYGGFDTLELSEAIFCADSKIIPNLLTLIKD
                     TNNDWKVDDVSILVNYLYLKCFFQNDNKKILNFLNLVSTKKVKENVNEKIEHYLKLLK
                     VNNLGDQIFYDKNFKELKHAIKNLFLKMIAQDFELQKVYSIIDSIIHVHNNRLIGIER
                     DKEKLIYYTLQRLFVSEEYMK"
     CDS             4431..6233
                     /function="transport of Nisin"
                     /gene="nisT"
                     /codon_start=1
                     /translation="MDEVKEFTSKQFFYTLLTLPSTLKLIFQLEKRYAIYLIVLNAIT
                     AFVPLASLFIYQDLINSVLGSGRHLINIIIIYFIVQVITTVLGQLESYVSGKFDMRLS
                     YSINMRLMRTTSSLELSDYEQADMYNIIEKVTQDSTYKPFQLFNAIIVELSSFISLLS
                     SLFFIGTWNIGVAILLLIVPVLSLVLFLRVGQLEFLIQWQRASSERETWYIVYLLTHD
                     FSFKEIKLNNISNYFIHKFGKLKKGFINQDLAIARKKTYFNIFLDFILNLINILTIFA
                     MILSVRAGKLLIGNLVSLIQAISKINTYSQTMIQNIYIIYNTSLFMEQLFEFLKRESV
                     VHKKIEDTEICNQHIGTVKVINLSYVYPNSNAFALKNINLSFEKGELTAIVGKNGSGK
                     STLVKIISGLYQPTMGIIQYDKMRSSLMPEEFYQKNISVLFQDFVKYELTIRENIGLS
                     DLSSQWEDEKIIKVLDNLGLDFLKTNNQYVLDTQLGNWFQEGHQLSGGQWQKIALART
                     FFKKASIYILDEPSAALDPVAEKEIFDYFVALSENNISIFISHSLNAARKANKIVVMK
                     DGQVEDVGSHDVLLRRCQYYQELYYSEQYEDNDE"
     CDS             6214..7470
                     /gene="nisC"
                     /note="biosynthetic gene"
                     /codon_start=1
                     /translation="MRIMMNKKNIKRNVEKIIAQWDERTRKNKENFDFGELTLSTGLP
                     GIILMLAELKNKDNSKIYQKKIDNYIEYIVSKLSTYGLLTGSLYSGAAGIALSILHLR
                     EDDEKYKNLLDSLNRYIEYFVREKIEGFNLENITPPDYDVIEGLSGILSYLLLINDEQ
                     YDDLKILIINFLSNLTKENNGLISLYIKSENQMSQSESEMYPLGCLNMGLAHGLAGVG
                     CILAYAHIKGYSNEASLSALQKIIFIYEKFELERKKQFLWKDGLVADELKKEKVIREA
                     SFIRDAWCYGGPGISLLYLYGGLALDNDYFVDKAEKILESAMQRKLGIDSYMICHGYS
                     GLIEICSLFKRLLNTKKFDSYMEEFNVNSEQILEEYGDESGTGFLEGISGCILVLSKF
                     EYSINFTYWRQALLLFDDFLKGGKRK"
BASE COUNT     2744 a    943 c   1332 g   2481 t
ORIGIN      
        1 aagcttgtgg gaaagaacat cctcagcctg gcttgattgt ccatactgat caagggagtc
       61 aatatacaag ctctcgttat caatctactc ttcgtcaagt cggtgctcaa tctagcatga
      121 gtcgtaaagg aaatccctat gacaatgcaa tgatggagtc tttttataag acgctaaaga
      181 gggagcttat taatgatgct cattttgaga caagagctga ggctactcaa gaaatattta
      241 aatacattga gacctattac aatacaaaaa ggatgcattc aggtcttgat tacaagtctc
      301 caaaagactt tgaaaaatat aattcttaaa ttctcttaac tccgtgtcta gtttttcgtt
      361 gactttccat tatgcttgga ttttttattg tttaattccc ttttttgtat acaagctcgt
      421 attcttaaca aataattggc atatcgggtt taaaaatact atgtgtttta aagaatctct
      481 catgagtttg acgccaataa cttagattaa aatcattgtc accttatttt taggcacgtt
      541 cggcagtaac cttatcaaag gtatctcggt cattaagttt catgatagta tttactattt
      601 tgatggtttt tgttattatc caatcgttaa aatgacaaaa acaaatagat aaatagataa
      661 atatttatgg ggaggacaag tgaacttatc atgattaatt gtaaacgatt gagttctgat
      721 atgtttcaaa ttatgaggaa caacaggagt tggactattc tttaaacgcc tcgacgatac
      781 catcactctt cattagccta aaattaacaa gttaaaatca ttagaataat ctcttttaca
      841 aaaaatattt atttaagtta tagttgacga atatttaata attttattaa tatcttgatt
      901 ttctagttcc tgaataatat agagataggt ttattgagtc ttagacatac ttgaatgacc
      961 tagtcttata actatactga caatagaaac attaacaaat ctaaaacagt cttaattcta
     1021 tcttgagaaa gtattggtaa taatattatt gtcgataacg cgagcataat aaacggctct
     1081 gattaaattc tgaagtttgt tagatacaat gatttcgttc gaaggaacta caaaataaat
     1141 tataaggagg cactcaaaat gagtacaaaa gattttaact tggatttggt atctgtttcg
     1201 aagaaagatt caggtgcatc accacgcatt acaagtattt cgctatgtac acccggttgt
     1261 aaaacaggag ctctgatggg ttgtaacatg aaaacagcaa cttgtcattg tagtattcac
     1321 gtaagtaaat aaccaaatca aaggatagta ttttttagtt cagatatgga tactatccta
     1381 tttttataag ttatttaggg ttgctaaata gcttataaaa ataaagagag gaaaaaacat
     1441 gataaaaagt tcatttaaag ctcaaccgtt tttagtaaga aatacaattt tatgtccaaa
     1501 cgataaacgg agttttactg aatatactca agtcattgag actgtaagta aaaataaagt
     1561 ttttttggaa cagttactac tagctaatcc taaactctat gatgttatgc agaaatataa
     1621 tgctggtctg ttaaagaaga aaagggttaa aaaattattt gaatctattt acaagtatta
     1681 taagagaagt tatttacgat caactccatt tggattattt agtgaaactt caattggtgt
     1741 tttttcgaaa agttcacagt acaagttaat gggaaagact acaaagggta taagattgga
     1801 tactcagtgg ttgattcgcc tagttcataa aatggaagta gatttctcaa aaaagttatc
     1861 atttactaga aataatgcaa attataagtt tggagatcga gtttttcaag tttataccat
     1921 aaatagtagt gagcttgaag aagtaaatat taaatatacg aatgtttatc aaattatttc
     1981 tgaattttgt gagaatgact atcaaaaata tgaagatatt tgtgaaactg taacgctttg
     2041 ctatggagac gaatatagag aactatcgga acaatatctt ggcagtctga tagttaatca
     2101 ttatttgatc tctaatttac aaaaagattt gttgtcagat ttttcttgga acactttttt
     2161 gactaaagtt gaagcaatag atgaagataa aaaatatata attcctctga aaaaagttca
     2221 aaagtttatt caagaatact cagaaataga aattggtgaa ggtattgaga aactgaaaga
     2281 aatatatcag gaaatgtcac aaattcttga gaatgataat tatattcaaa ttgatttaat
     2341 tagtgatagt gaaataaatt ttgatgttaa acaaaagcaa caattagaac atttagctga
     2401 gtttttagga aatacgacaa aatctgtaag aagaacatat ttggatgact ataaggataa
     2461 atttatcgaa aaatatggtg tagatcaaga agtacaaata acagaattat ttgattctac
     2521 atttggcata ggagctccat ataattataa tcatcctcga aatgactttt atgagtccga
     2581 accgagtact ctatactatt cagaagagga gagagaaaag tacctcagca tgtatgtaga
     2641 agccgttaaa aatcataatg taattaatct tgacgactta gagtctcatt atcaaaaaat
     2701 ggacttagaa aagaaaagtg aacttcaagg gttagaatta tttttgaatt tggcaaagga
     2761 gtatgaaaaa gatattttta ttttagggga tatcgttgga aataataatt tgggaggggc
     2821 atcaggtaga ttttctgcac tctctccgga gttaacaagt tatcatagaa cgatagtaga
     2881 ttctgtcgaa agagaaaatg agaataaaga aattacatcg tgtgaaatag tatttcttcc
     2941 agaaaatatc agacatgcta acgttatgca tacatcaatt atgaggagga aagtacttcc
     3001 attttttaca agtacaagtc acaatgaagt tctgttaact aatatctata ttggaataga
     3061 cgaaaaagaa aaattttatg cacgagacat ttcaactcaa gaggtattga aattctacat
     3121 tacaagcatg tacaataaaa cgttattcag taatgagcta agatttcttt acgaaatttc
     3181 attagatgac aagtttggta atttaccttg ggaacttatt tacagagact ttgattatat
     3241 tccacgttta gtatttgacg aaatagtaat atctcctgct aaatggaaaa tttggggaag
     3301 ggatgtaaat agtaagatga caataagaga acttattcaa agcaaagaaa ttcccaaaga
     3361 gttttatatt gtcaatggag ataataaagt ttatttatca cagaaaaacc cattggatat
     3421 ggaaatttta gagtcggcga taaagaagag ctcaaaaaga aaagatttta tagagctaca
     3481 agaatatttt gaagatgaaa atatcataaa taaaggagaa aaggggagag ttgccgatgt
     3541 tgtagtgcct tttattagaa cgagagcatt aggtaatgaa gggagagcat ttataagaga
     3601 gaaaagagtt tcggttgaac ggcgtgaaaa attgcccttt aacgagtggc tttatctaaa
     3661 gttgtacatt tctataaatc gtcaaaatga atttttactg tcgtatcttc cagatattca
     3721 gaaaatagta gcaaacctgg gtggaaatct attcttccta agatatactg atcctaaacc
     3781 acatattaga ttgcgtataa aatgttcaga tttattttta gcttacggat ctattcttga
     3841 aatcttaaaa aggagtcgga aaaataggat aatgtcaact tttgatattt ctatttatga
     3901 tcaagaagta gaaagatatg gtggatttga tactttagag ttatccgaag caatattttg
     3961 tgccgattct aaaattattc caaatttgct tacattgata aaagatacta ataatgattg
     4021 gaaagtcgat gatgtatcaa tcttggtgaa ttatttatat ctgaaatgct tctttcagaa
     4081 tgataacaaa aagattctta attttttgaa tttagttagt actaaaaagg ttaaagaaaa
     4141 tgtcaatgaa aagattgaac attatcttaa gcttctgaaa gttaataatc taggtgacca
     4201 aattttttat gacaagaatt ttaaagaatt aaagcatgcc ataaaaaatt tatttttaaa
     4261 aatgatagct caagattttg aacttcagaa agtttattca attattgaca gtatcattca
     4321 tgtccataat aaccgactaa ttggtattga acgagataaa gagaaattaa tttattacac
     4381 acttcaaagg ttgtttgttt cggaagaata catgaaatga ggactaatag atggatgaag
     4441 tgaaagaatt cacatcaaaa caattttttt atactttact tactcttcca agcaccttga
     4501 agttaatttt tcagttggaa aaacgttatg caatttattt aattgtgcta aatgctatca
     4561 cagcttttgt tccgttggct agtcttttta tttatcaaga tttaataaac tctgtgctag
     4621 gttcagggag acatcttatc aatattatta tcatctattt tattgttcaa gtgataacaa
     4681 cagttctggg acagctggaa agttatgtta gtggaaaatt tgatatgcga ctttcttaca
     4741 gtatcaatat gcgcctcatg aggactacct catctcttga attaagtgat tatgagcagg
     4801 ctgatatgta taatatcata gaaaaagtta ctcaagacag cacttacaag ccttttcagc
     4861 tatttaatgc tatcattgtt gagctttcat cgtttatctc attgttatct agtctatttt
     4921 ttattggaac atggaacatt ggggtagcaa ttttactcct tattgttcca gtattatctt
     4981 tggtactttt tctcagagtg ggacaattag agtttttaat ccagtggcag agagcaagtt
     5041 ctgaaagaga aacatggtat attgtatatt tattgactca tgatttttca tttaaagaaa
     5101 tcaagttaaa taatattagc aattacttca ttcataaatt tggaaaatta aagaaaggat
     5161 ttatcaacca agatttagct attgctcgta agaagacata tttcaatatt tttcttgatt
     5221 tcattttgaa tttgataaat attcttacga tatttgctat gatcctttcg gtaagagcag
     5281 gaaaacttct tataggtaat ttggtaagtc tcatacaagc tatttctaaa atcaatactt
     5341 attctcaaac aatgattcaa aatatttaca tcatttataa tactagtttg tttatggaac
     5401 aactttttga gtttttaaag agagaaagtg tagttcacaa aaaaatagaa gatactgaaa
     5461 tatgcaatca acatatagga actgttaaag taattaattt atcatatgtt taccctaatt
     5521 cgaatgcctt tgcactaaag aatatcaatt tatcctttga aaaaggagaa ttaactgcta
     5581 ttgtaggaaa aaatggttca gggaaaagta cactagtaaa gataatttca ggattatatc
     5641 aaccaactat gggaataatc caatacgaca aaatgagaag tagtttgatg cctgaggagt
     5701 tttatcagaa aaacatatcg gtgctgttcc aagattttgt gaagtatgag ttaacgataa
     5761 gagagaatat aggattgagt gatttgtctt ctcaatggga agatgagaaa attattaaag
     5821 tactagataa tttaggactc gattttttga aaactaataa tcaatatgta cttgatacgc
     5881 agttaggaaa ttggtttcaa gaagggcatc aactttcagg aggtcagtgg caaaaaattg
     5941 cattagcaag gacattcttt aagaaagctt caatttatat tttagatgaa ccaagtgctg
     6001 cactcgatcc tgtagctgaa aaagaaatat ttgattattt tgttgctctt tcggaaaata
     6061 atatttcaat tttcatttct catagtttga atgctgccag aaaagcaaat aaaatcgtgg
     6121 ttatgaaaga tggacaggtc gaagatgttg gaagtcatga tgtccttctg agaagatgtc
     6181 aatactatca agaactttat tattcagagc aatatgagga taatgatgaa taaaaaaaat
     6241 ataaaaagaa atgttgaaaa aattattgct caatgggatg agagaactag aaaaaataaa
     6301 gaaaacttcg atttcggaga gttgactctc tctacaggat tgcctggtat aattttaatg
     6361 ttagcggagt taaaaaataa agataactca aagatatatc agaaaaagat agacaattat
     6421 attgaatata ttgttagcaa actttcaaca tatgggcttt taacaggatc actttattcg
     6481 ggagcagctg gcattgcatt aagtatccta catttacgag aagatgacga aaaatataag
     6541 aatcttcttg atagcctaaa tagatatatc gaatatttcg tcagagaaaa aattgaagga
     6601 tttaatttgg aaaacattac tcctcctgat tatgacgtga ttgaaggttt atctgggata
     6661 ctttcctatc tattattaat caacgacgag caatatgatg atttgaaaat actcattatc
     6721 aattttttat caaatctgac taaagaaaac aatggactaa tatcgcttta catcaaatcg
     6781 gagaatcaga tgtctcaatc agaaagtgag atgtatccac taggctgttt gaatatggga
     6841 ttagcacatg gacttgctgg agtgggctgt atcttagctt atgcccacat aaaaggatat
     6901 agtaatgaag cctcgttgtc agctttgcaa aaaattattt ttatttatga aaagtttgaa
     6961 cttgaaagga aaaaacagtt tctatggaaa gatggacttg tagcagatga attaaaaaaa
     7021 gagaaagtaa ttagggaagc aagtttcatt agagatgcat ggtgctatgg aggtccaggt
     7081 attagtctgc tatacttata cggaggatta gcactggata atgactattt tgtagataaa
     7141 gcagaaaaaa tattagagtc agctatgcaa aggaaacttg gtattgattc atatatgatt
     7201 tgccatggct attctggttt aatagaaatt tgttctttat ttaagcggct attaaataca
     7261 aaaaagtttg attcatacat ggaagaattt aatgttaata gtgagcaaat tcttgaagaa
     7321 tacggagatg aaagtggcac gggttttctt gaaggaataa gtggctgtat actggtatta
     7381 tcgaaatttg aatattcaat caattttact tattggagac aagcactgtt actttttgac
     7441 gattttttga aaggagggaa gaggaaatga gaagatattt aatacttatt gtggccttaa
//