From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Pseudomonas sp. lipase, complete cds.
Date: 26 Aug 92 10:00:48 GMT
Distribution: bionet
Checksum: 20746     4

LOCUS       PSELIPAS     1877 bp ds-DNA             BCT       26-AUG-1992
DEFINITION  Pseudomonas sp. lipase, complete cds.
ACCESSION   M74125
KEYWORDS    lipase.
SOURCE      Pseudomonas sp. (individual_isolate LS107d2) DNA.
  ORGANISM  Pseudomonas sp.
            Prokaryotae; Gracilicutes; Scotobacteria; Pseudomonadaceae.
REFERENCE   1  (bases 1 to 1877)
  AUTHORS   Johnson,L.A., Beacham,I.R. and MacRae,I.C.
  TITLE     Degradation of triglycerides by a pseudomonad isolated from milk :
            Identification and nucleotide sequence of a lipase-encoding gene
  JOURNAL   Appl. Environ. Microbiol. (1991) In press
  STANDARD  simple staff_entry
FEATURES             Location/Qualifiers
     RBS             33..36
     CDS             44..1468
                     /product="lipase"
                     /codon_start=1
                     /translation="MGVFDYKNLGAEGSKALFADAMAITLYTYHNLDNGFAVGYQHNG
                     LGLGLPATLVGALLGSSDSQGVIPGIPWNPDSEKAALEAVQHAGWTPITASALGYTGK
                     VDARGTFFGEKPGYTTAQVEVLGKYDDAGKLLEIGIGFRGTSGPRESLISDSIGDLVQ
                     RSARGPGAQGLREKLRRRTFGGLLKNIADYASAHGLSGHEVVVSGHSLGGLAVNSMAD
                     LSNGKWAGFFKDAKYVAYASPTQSSGDKVLNVGYENDPVFRALDGSSVNWSSLGVHDK
                     PHESTTDNIVSFNDHYASTLWNVLPFSITNLPTWISHLPTGYGDGMTRVLESGFYEVM
                     TRDSTIIVSNLSDPARANTWVQDLNRNAEPHKGDTFIIGSAGNDLIQGGKGADFIEAG
                     KGNDTIRDSSGHNTFLFSGQFGQDRIIGYQPTDKLVFTDVQSSGDYRDHAKVVGGDTV
                     ISFGGDSVTLVGVVGLSGEGIVIS"
BASE COUNT      393 a    524 c    586 g    374 t
ORIGIN
        1 aaccagcttt ctcgacaact ccaacaaaaa gagaggcagt accatgggtg tattcgacta
       61 taaaaacctg ggcgccgaag gctccaaagc gttgttcgcc gatgccatgg cgatcacgct
      121 gtacacctac cacaacctgg ataacggctt tgccgtgggt tatcagcaca acggtctagg
      181 ccttggcttg ccggccaccc tggtcggcgc gttgttaggc agcagtgatt cccagggcgt
      241 gatccccggc attccctgga accccgactc ggaaaaagcc gccctggagg ccgtgcaaca
      301 cgccggctgg acacctatca cggccagcgc cctgggctat accggcaagg tcgacgccag
      361 gggcaccttc tttggcgaaa aaccgggcta caccacggcc caggtcgaag tgctcggcaa
      421 gtacgatgac gccggcaagc tgctggaaat tggcatcggt tttcgtggta cttcaggccc
      481 acgggaaagc ctgatcagcg actccatcgg cgatctggtc cagcgatctg ctcgcggccc
      541 tggggcccaa ggattacgcg aaaaactacg ccggcgaacg ttcggcggtt tgctcaagaa
      601 catcgctgac tacgccagtg cccacggcct cagcggccac gaggtggtag tcagcggcca
      661 cagcctgggt ggccttgcgg tgaacagcat ggcggacttg agcaacggta aatgggcggg
      721 cttcttcaag gacgccaagt atgtggccta cgcctcgccg acccagagca gcggcgacaa
      781 ggtgctcaat gtcggctatg aaaacgatcc ggttttccgt gcgctggacg gctcatccgt
      841 caactggtcg tcgttgggcg tgcatgacaa gccccatgaa tcgaccaccg acaatatcgt
      901 cagcttcaac gaccattacg cctcgacgct gtggaatgtg ctgccgtttt ccatcaccaa
      961 cctgccgacc tggatctccc atttgcccac cggatatggc gacggcatga cgcgtgttct
     1021 ggagtccggg ttctatgagg tgatgactcg cgactcgacg attattgtct ccaacctgtc
     1081 ggaccctgcg cgggccaaca cgtgggtgca ggacctcaac cgcaacgccg agccccacaa
     1141 aggcgacacc ttcatcattg gcagcgctgg caatgacttg atccagggcg gcaagggcgc
     1201 ggactttatc gaggccggca agggtaacga cacgatccgt gacagcagcg ggcacaacac
     1261 gtttttattc agcgggcagt ttggccagga ccggattatt ggctatcagc cgacggacaa
     1321 attggtgttt acggacgtgc agagcagtgg cgattatcgt gatcacgcca aggtggtggg
     1381 cggggatacc gtgatcagtt ttggcgggga ttcagtgacg ctggtgggag tggttgggtt
     1441 gtcgggggag gggattgtca tcagttaaaa cggtgggagg ggggttgctc ccgatagcgg
     1501 tggttcagtc aatggagatg ttgactggaa gatcgccatc gggggcaagc cccctcccac
     1561 atttgggcag tgttggcagc ggagaatcag tcttcctttc cgcatcagtg ggcagcggac
     1621 gctattggtc caaataggaa cgcggtcaac cctgtggagg gggcttgctt gccccgatgg
     1681 cgttggttca gtcaatggag atgttgacta agatcgccat cgggagcaag ccccctccca
     1741 catttgggca gtgttggcag cggagaatca gtcttccttc cgcatcagtg ggcagcggac
     1801 gctattggtc aaattggaac gcggtcaacc tgtgggaggg gcttgccccc gatggcggtg
     1861 gctcagtcaa taaagat
//