From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human pyruvate dehydrogenase complex (PDHA2) gene, complete cds.
Date: 30 Jul 92 10:01:21 GMT
Distribution: bionet
Checksum: 50832     7

LOCUS       HUMPYRDH     3055 bp ds-DNA             PRI       30-JUL-1992
DEFINITION  Human pyruvate dehydrogenase complex (PDHA2) gene, complete cds.
ACCESSION   M86808
KEYWORDS    pyruvate dehydrogenase complex.
SOURCE      Homo sapiens (library: ATCC 57718) Adult DNA.
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (sites)
  AUTHORS   Dahl,H.-H.M., Brown,R.M., Hutchison,W.M., Maragos,C.D. and
            Brown,G.D.
  TITLE     A testis-specific form of the human pyruvate dehydrogenase E1a
            subunit is coded for by an intronless gene on chromosome 4
  JOURNAL   Genomics 8, 225-232 (1990)
  STANDARD  full staff_review
REFERENCE   2  (bases 1 to 3055)
  AUTHORS   Dahl,H.-H.M.
  TITLE     Human testis-specific pyruvate dehydrogenase E1a gene (PDHA2)
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
REFERENCE   3  (sites)
  AUTHORS   Fitzgerald,J., Hutchison,W.M. and Dahl,H.-H.M.
  TITLE     Isolation and characterization of the mouse PDH E1a genes
  JOURNAL   Biochim. Biophys. Acta 1131, 83-90 (1992)
  STANDARD  full staff_review
REFERENCE   4  (sites)
  AUTHORS   Fitzgerald,J., Hutchison,W.M. and Dahl,H.-H.M.
  TITLE     Isolation and characterization of the mouse pyruvate dehydrogenase
            E1 alpha genes
  JOURNAL   Biochim. Biophys. Acta 1131, 83-90 (1992)
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     repeat_unit     989..1000
                     /rpt_type=direct
                     /gene="PDHA2"
     CDS             1631..2797
                     /EC_number="1.2.4.1"
                     /standard_name="testis-specific pyruvate dehydrogenase E1a
                     subunit"
                     /product="pyruvate dehydrogenase complex"
                     /gene="PDHA2"
                     /codon_start=1
                     /translation="MLAAFISRVLRRVAQKSARRVLVASRNSSNDATFEIKKCDLYLL
                     EEGPPVTTVLTRAEGLKYYRMMLTVRRMELKADQLYKQKFIRGFCHLCDGQEACCVGL
                     EAGINPSDHVITSYRAHGVCYTRGLSVRSILAELTGRRGGCAKGKGGSMHMYTKNFYG
                     GNGIVGAQGPLGAGIALACKYKGNDEICLTLYGDGAANQGQIAEAFNMAALWKLPCVF
                     ICENNLYGMGTSTERAAASPDYYKRGNFIPGLKVDGMDVLCVREATKFAANYCRSGKG
                     PILMELQTYRYHGHSMSDPGVSYRTREEIQEVRSKRDPIIILQDRMVNSKLATVEELK
                     EIGAEVRKEIDDAAQFATTDPEPHLEELGHHIYSSDSSFEVRGANPWIKFKSVS"
     polyA_signal    2885..2890
                     /gene="PDHA2"
     polyA_signal    2934..2939
                     /gene="PDHA2"
     repeat_unit     2969..2978
                     /rpt_type=direct
                     /gene="PDHA2"
BASE COUNT      939 a    566 c    707 g    843 t
ORIGIN      chromosome 4, map position 4q22-23
        1 gaattccaac agttgaattt tcaattgagt gaaaagatga ggcctattat gatggctggg
       61 ttcagaaggt ggggagacga tcaagtttta agaggtatct taacttttta acaagctatt
      121 ttctcttttc ttcctgaata ttaaaagaag tgcccaaaat aacacctgca actagatata
      181 gagaaggcag ctgctttgaa catgtaatag ggacatttgg gtaagaattt atcatttgca
      241 aataggcaat ggatataaaa cagagcatgc attaaggtga atggcttaat attctaaata
      301 aaacaggatc cctagttagg gttggcttat tggggcacaa aatcgccttc ctggtaaaag
      361 cacaaaaaga gagaaatgat atgtgatctt tgtatccctt ggtctaaggc ttctcttctc
      421 ttgtgattga acggtttcca gtggcataag caaactctgt tgctctcttg tagtccaaac
      481 tgtgtattgt ttggtgggtc actttataag aagttacact ttctaatggt tactacataa
      541 aaaggcacta taagaacatg tttctagatt tcctggacta aggctggtcc agtttgattg
      601 atatgaaagc aaaataaata aataaataaa taaataccac ctgaaagtct atggaaacct
      661 gctgaagaca tttttataga aaaccaaaaa gaaaaaaaag aggaaataaa caaaaggaag
      721 agcaaaagtg aagtagtcag aaaataggga taaaactagg taaaaacaga gattcagaat
      781 tattaaacag agtaaggaaa agtggaatgt cacaaactac aaacttttta ccacaatctg
      841 cacctcaacc ctctctccct gcttccaatt ttcccacacc aagcaaagtt tattactggg
      901 tttaaagtgt tatcctcagg ccgaggtttg tatacatttt ccctccccac ttcaagtgtc
      961 acaggtcgta gacgccatca ggataaatgt ggcatattat ttgagagtac tgacttttct
     1021 ttaaaagggg tttttgttaa cgtgcgtgtg cttgttttgt ttttaaagtt aattaagcta
     1081 caaataattt acagctttgt tgttacctta aaatacatat ggcacattat ggagcaggat
     1141 aaatattcat acctatttta aagatcaaaa acaagacatt aaagaaatat tggtaggaag
     1201 aaataccttt ggactaactc tcagaactct catgtgcctt agggactgag tttgttcctt
     1261 tcttctattt gatacgcttg tcgggaaagc ttgagataca gtgctccagg gtttaacagg
     1321 gaaaagggac tcagattatt gaggttcagc aatgacggcg attaggatgc cctgtagttt
     1381 gcccaagtcc tccagaaaag gaaatcactt ttagtcttca ttttacagct ggcaggcact
     1441 gtacaaatca gtcagctcag gctgcgtggc ccatgtccca gtgctgtgca ggacctgcct
     1501 ctatcacccg tgtgcactgc ccggatggtg ccaagcgcat tggagcaccg gaagagacgc
     1561 ggacgcgagg ccaggccttc cggcggtcgt tacgggacgc cgctgccatc tacagcactc
     1621 cgtgaagaat atgctggccg ccttcatctc ccgcgtgttg aggcgagttg cccagaaatc
     1681 agctcgcaga gtgctggtgg catcccgtaa ctcctcaaat gacgctacat ttgaaattaa
     1741 gaaatgtgat ctttatctgt tggaagaggg tccccctgtc actacagtgc tcactagggc
     1801 ggaggggctt aaatactaca ggatgatgct gactgttcgc cgcatggaat tgaaggcaga
     1861 tcagctgtac aaacagaaat tcattcgcgg tttctgtcac ctgtgcgatg gtcaggaagc
     1921 ttgttgcgtg ggccttgagg ccggcataaa cccctcggat cacgtcatta catcctatag
     1981 ggctcatggt gtgtgctata ctcggggact ttctgtccga tccattctcg cagagctgac
     2041 gggaagaaga ggaggttgtg ctaaaggaaa aggaggatcg atgcatatgt ataccaagaa
     2101 cttctatggg ggcaatggca tcgtcggtgc acagggcccc ctgggcgctg gcattgctct
     2161 ggcctgtaaa tataaaggaa acgatgagat ctgtttgact ttatatgggg atggcgctgc
     2221 gaatcagggg cagatagccg aagctttcaa tatggcagct ttatggaaat taccttgtgt
     2281 tttcatctgt gagaataacc tatatggaat gggaacatct actgagagag cagcagccag
     2341 ccctgattac tacaagaggg gcaattttat ccctgggcta aaggtcgatg gaatggatgt
     2401 tctgtgtgtt cgtgaggcaa caaaatttgc agctaactac tgtagatctg gaaaggggcc
     2461 catactgatg gagctgcaaa cctaccgtta tcatggacac agtatgagtg atcctggagt
     2521 cagttatcgt acacgagaag aaattcagga agtaagaagt aagagggatc ctataataat
     2581 tctccaagat agaatggtaa acagcaagct cgccactgtg gaagaattaa aggaaattgg
     2641 ggctgaggtg aggaaagaaa ttgatgatgc tgcccagttt gctaccactg atcctgagcc
     2701 acatttggaa gaattaggcc atcacatcta cagcagtgat tcatcttttg aagttcgtgg
     2761 tgcaaatcca tggatcaagt ttaagtccgt cagttaaagg gaggctacgt gtgaatttat
     2821 catcagtctc tcaatggaat gttcatggtc aaatttaaga aactgtgttc tcaactcaag
     2881 gaggaataaa actcataaaa caaaagcctt gtaagcattt attaaaagag attattaaaa
     2941 gagattgaaa gacatgcgtt caataaaagt gatattatat ttcaattata ctgtatattt
     3001 ttgcattaaa agatactata taggtaataa tttagtgtga atatcttaaa gatta
//