From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Plasmid pSa (from Escherichia coli) dihydropteroate synthase gene,
Date: 21 Dec 92 20:50:53 GMT
Distribution: bionet
Checksum: 64043 7

LOCUS       PLPDHOS      3119 bp ds-DNA             BCT       20-NOV-1992
DEFINITION  Plasmid pSa (from Escherichia coli) dihydropteroate synthase gene,
            3' end.
ACCESSION   L06822
KEYWORDS    dihydropteroate synthase.
SOURCE      Plasmid pSa DNA.
  ORGANISM  Plasmid pSa
            Prokaryotae.
REFERENCE   1  (bases 1 to 3119)
  AUTHORS   Stokes,H.W., Tomaras,C., Parsons,Y. and Hall,R.M.
  TITLE     The partial 3'-conserved segment duplications in the integrons In6
            from pSa and In7 from pDGO100 have a common origin
  JOURNAL   Plasmid (1993) In press
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     misc_feature    1..508
                     /standard_name="'3'-conserved segment'"
                     /note="'bases 807-1355 of the 3'-conserved segment'"
     misc_feature    509..2613
                     /note="'region also found in In7 from pDGO100'"
     -35_signal      640..645
                     /citation=[1]
     -10_signal      662..667
                     /citation=[1]
     RBS             833..837
                     /citation=[1]
     misc_feature    2614..3006
                     /note="'duplication of bases 2218..2613'"
     CDS             <1..484
                     /note="aa120-279"
                     /product="dihydropteroate synthase"
                     /codon_start=2
                     /translation="CRLVVMHSAQRDGIATRTGHLRPEDALDEIVRFFEARVSALRRS
                     GVAADRLILDPGMGFFLSPAPETSLHVLSNLQKLKSALGLPLLVSVSRKSFLGATVGL
                     PVKDLGPASLAAELHAIGNGADYVRTHAPGDLRSAITFSETLAKFRSRDARDRGLDHA
                     "
     CDS             844..1869
                     /citation=[1]
                     /product="'ORF341'"
                     /codon_start=1
                     /translation="MSLARNATASQSPTQTNGYERHQPDQTLLYQLVEQHYPAFKASL
                     EAQGQHLPRYIQQEFNDLLQCGRLEYGFMRVRCEDCHHERLVAFSCKRRGFCPSCGAR
                     RMAESAALLIDEVFPKEPIRQWVLSFPFQLRFLLARHPQLMGQVLSIVYRTLSTHLIK
                     KAGYTKASAQTGSVTLIQRFGSALNLNVHYHMLFLDGVYAEDDYGKQRFHRVKAPTYD
                     ELNTLAHTLSHRIARCMEKRGILERDAENTWLTLEEGEDDTLTQLHGASVTYRIAVGP
                     QQGRKVFTLQTLPGREDKADSSSRVANHAGFSLHAGVMAEAHQRISLSACVATLVGQR
                     FQKNVWH"
BASE COUNT      750 a    871 c    812 g    686 t
ORIGIN      
        1 ctgcaggctg gtggttatgc actcagcgca gcgggatggc atcgccaccc gcaccggtca
       61 ccttcgaccc gaagacgcgc tcgacgagat tgtgcggttc ttcgaggcgc gggtttccgc
      121 cttgcgacgg agcggggtcg ctgccgaccg gctcatcctc gatccgggga tgggattttt
      181 cttgagcccc gcaccggaaa catcgctgca cgtgctgtcg aaccttcaaa agctgaagtc
      241 ggcgttgggg cttccgctat tggtctcggt gtcgcggaaa tccttcttgg gcgccaccgt
      301 tggccttcct gtaaaggatc tgggtccagc gagccttgcg gcggaacttc acgcgatcgg
      361 caatggcgct gactacgtcc gcacccacgc gcctggagat ctgcgaagcg caatcacctt
      421 ctcggaaacc ctcgcgaaat ttcgcagtcg cgacgccaga gaccgagggt tagatcatgc
      481 ctagcattca ccttccggcc gcccgctaaa tatctccttt tgggttgtta ataaaacatc
      541 caataagttg actgtgcgtg aaaaagaaag ttttgtgtga tggcgttgaa gatcgcaccg
      601 ttaagctctt atgtgggatg gtgcagagct cgacgactac cgataaaacg caaccgccca
      661 gtattgtgat tggtggagca atagcaccct gaacccaaaa ccttctcgct caaccggtag
      721 tggctgataa caactcgtga gggctattgc gggttaagca tttagcgatg tctagggcca
      781 gactggacgt ctgaacgcaa gccgctgata ctgtacataa ccacagtatc agggaggata
      841 cccatgtcgc tggcaaggaa cgccacggcg agtcaatcgc ccactcaaac aaacggttac
      901 gaacgccacc aacccgacca gacgctgctc taccagctgg ttgagcagca ctacccagcc
      961 ttcaaagcct cactcgaagc ccaaggtcaa cacctgcctc gctacatcca acaagaattc
     1021 aacgacctcc tccaatgtgg ccgtctggag tatggtttca tgcgggttcg ctgcgaggat
     1081 tgtcatcacg agcgtctggt cgccttcagc tgtaaacgac gcggcttttg ccctagctgc
     1141 ggtgcccgcc ggatggccga gagtgcggcg ctgctgatag acgaagtctt ccccaaggag
     1201 cccattcgcc agtgggtgct cagctttcct ttccagctac gctttttgct ggctcgccat
     1261 ccccagctga tgggccaggt cttgagtatc gtctatcgta cactctcaac tcatctgatc
     1321 aaaaaagccg gttacaccaa agcctctgca caaactggct cagtgactct tatccaacgc
     1381 tttggctccg cgctaaatct caatgtccac taccacatgc tgtttctcga tggtgtctat
     1441 gccgaagatg actatggcaa gcaacgcttc catcgtgtca aggcacccac ttacgatgag
     1501 ctgaatacgc tcgctcacac cctcagccat cgcatcgctc gctgcatgga aaagcgtggg
     1561 attttggagc gtgatgccga gaatacgtgg ttgacactgg aagagggcga agacgatacg
     1621 ctgactcaat tacatggtgc ttcggttacg tatcgcattg ccgtcggccc ccagcaaggg
     1681 cgcaaagtct tcaccctgca aaccttgcca gggcgtgagg ataaagccga ctcaagcagt
     1741 cgagtagcca accatgctgg tttctcgcta cacgccggtg tgatggccga agcgcatcag
     1801 cggataagct tgagcgcttg tgtcgctaca ttagtcggcc agcggtttca gaaaaacgtc
     1861 tggcattaac cgccaatggg caggtgcgtt acgagctcaa aactccgtac cgcaatggca
     1921 ccacccatgt gatcttcgag ccgctggact tcatcgccaa actcgctgcg ttggtaccta
     1981 agccgcgagt caacctcaca cgcttccacg gcgtctttgc accgaacagc aaacaccgag
     2041 ttcaagtaac acccgccaag cggcaagaag cccgacaaat cggaaggtct cgatactaac
     2101 tggcgtgaca agagtcctgc agagcgccac cgcgcatgac ctggatgcaa cgcctcaagc
     2161 gagtcttcaa tattgatatt gaagtctgcg aacactgcgg cggtcacgtc aaagtgattg
     2221 ccagcatcga agatccgaag gtcattgagc agattctcaa gcatctgaaa cagaaaacag
     2281 ccaaggcgaa tgccgccaag cagcgtgagc tgccaccaga acgagcgccg ccactgactc
     2341 ccagcctgtt cgatccatca cagagtcgtc tctttgactg acgaccccaa atccaacact
     2401 gctcaacact gccaactttt aaacggggcg gtggggcagt ttgtatctct cgagctatca
     2461 ggctagagat tttaccgcca aatcgaacct tattagagcg gtttaggctg gaccggcagt
     2521 taaaattggg gcttgagcgg taaacgagtg agggaatttc aggtaagata cttcggatga
     2581 ggagcaaaaa ggtggtttat acttcctata cccttgccag catcgaagat ccgaaggtca
     2641 ttgagcagat tctcaagcat ctgaaacaga aaacagccaa ggcgaatgcc gccaagcagc
     2701 gtgagctgcc agaacgagcg ccgccactga ctcccagcct gttcgatcca tcacagagtc
     2761 gtctctttga ctgacgaccc caaatccaac actgctcaac actgccaact tttaaacggg
     2821 gcggtggggc agtttgtatc tctcgagcta tcaggctaga gattttaccg ccaaatcgaa
     2881 ccttattaga gcggtttagg ctggaccggc agttaaaatt ggggcttgag cggtaaacga
     2941 gtgagggaat ttcaggtaag atacttcgga tgaggagcaa aaaggtggtt tatacttcct
     3001 atacccaaaa aggtggttta tacttcctat accccggtta tccggtggtt ttctgtttgc
     3061 ggaataaaac agattagtga cagttttctc agaatggcgt gttccggtga cttctgttt
//