From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Escherichia coli membrane-bound lytic transglycosylase (mlt) gene,
Date: 31 Dec 92 01:44:29 GMT
Distribution: bionet
Checksum: 20675 5

LOCUS       ECOMLT       1743 bp ds-DNA             BCT       18-DEC-1992
DEFINITION  Escherichia coli membrane-bound lytic transglycosylase (mlt) gene,
            complete cds; ORF gene, 3'end.
ACCESSION   M87660
KEYWORDS    membrane-bound lytic transglycosylase; mlt gene;
            open reading frame.
SOURCE      Escherichia coli (strain K-12) DNA.
  ORGANISM  Escherichia coli
            Prokaryotae; Gram-negative facultatively anaerobic rods;
            Enterobacteriaceae.
REFERENCE   1  (bases 1 to 1743)
  AUTHORS   Engel,H., Smink,A.J., Keck,W. and van Wijngaarden,L.
  TITLE     Murein-metabolizing enzymes from Escherichia coli: existence of a
            second lytic transglycosylase transglycosylase from escherichia
            coli
  JOURNAL   J. Bacteriol. 174, 6394-6403 (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     misc_feature    237..284
                     /gene="mlt"
                     /note="predicted transmembrane helix"
     misc_feature    432..482
                     /gene="mlt"
                     /note="predicted transmembrane helix"
     misc_feature    564..626
                     /gene="mlt"
                     /note="predicted membrane associated helix"
     CDS             168..1139
                     /gene="mlt"
                     /product="membrane-bound lytic transglycosylase"
                     /codon_start=1
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVDWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IARRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     CDS             complement(1359..1743)
                     /partial
                     /note="C-terminal ORF"
                     /codon_start=2
                     /translation="RGQYPSETVAAMARVCLGAEKIPSINVSKHRLDVQFDNVEEAIA
                     MSAMYAANHLKGVTAITMTESGRTALMTSRISSGLPIFAMSRHERTLNLTALYRGVTP
                     VHFDSANDGVAAASERLICCAIKVT"
BASE COUNT      425 a    423 c    476 g    419 t
ORIGIN      
        1 aatctggcgc aggccaaaga gattgtgccg cagctacggt ttgattaatt aacatccatt
       61 cgcagccggt acgcagtcag taccggcttt ttttatttgg tgcggggcaa gttgcgccgc
      121 tacactatca ccagattgat ttttgcctta tccgaaactg gaaaagcatg gaaacgaaaa
      181 aaaataatag cgaatacatt cctgagtttg ataaatcctt tcgccacccg cgctactggg
      241 gagcatggct gggcgtagca gcgatggcgg gtatcgcttt aacgccgcca aagttccgtg
      301 atcccattct ggcacggctg ggacgttttg ccggacgact gggaaaaagc tcacgccgtc
      361 gtgcgttaat caatctgtcg ctctgctttc cagaacgtag tgaagctgaa cgcgaagcga
      421 ttgttgatga gatgtttgcc accgcgccgc aagcgatggc aatgatggct gagttggcaa
      481 tacgcgggcc ggagaaaatt cagccgcgcg ttgactggca agggctggag atcatcgaag
      541 agatgcggcg taataacgag aaagttatct ttctggtgcc gcacggttgg gccgtcgata
      601 ttcctgccat gctgatggcc tcgcaagggc agaaaatggc agcgatgttc cataatcagg
      661 gcaacccggt ttttgattat gtctggaaca cggtgcgtcg tcgctttggc ggtcgtctgc
      721 atgcgagaaa tgacggtatt aaaccattca tccagtcggt acgtcagggg tactggggat
      781 attatttacc cgatcaggat catggcccag agcacagcga atttgtggat ttctttgcca
      841 cctataaagc gacgttgccc gcgattggtc gtttgatgaa agtgtgccgt gcgcgcgttg
      901 taccgctgtt tccgatttat gatggcaaga cgcatcgtct gacgattcag gtgcgcccac
      961 cgatggatga tctgttagag gcggatgatc atacgattgc gcggcggatg aatgaagaag
     1021 tcgagatttt tgttggtccg cgaccagaac aatacacctg gatactaaaa ttgctgaaaa
     1081 ctcgcaaacc gggcgaaatc cagccgtata agcgcaaaga tctttatccc atcaaataaa
     1141 aaagcctctc gcgaggagag gccttcgcct gatgataagt tcaagtttgc ttcagaatat
     1201 tcgaaatctg ttgaactatc attgaactgt aggccggatg tggcgttttc gccgcatccg
     1261 gcaacgtact tactctaccg ttaaaatacg cgtggtatta gtagaaccca cggtactcat
     1321 cacgtcgccc tgggtgacaa tcaccaggtc accagacatc aagtaacctt tatcgcgcag
     1381 cagattaacc gttcgctggc agctgctacg ccgtcattag cgctatcaaa gtgcaccggc
     1441 gtaacgccac gatagagagc agtcaggttc agcgtacgtt catggcgcga catggcgaaa
     1501 attggcagac cagagctgat acgggaggtc atcagcgcgg tacgacccga ttcggtcatg
     1561 gtgatcgccg taacgccttt caggtggtta gctgcgtaca ttgctgacat ggcaatagct
     1621 tcttccacat tgtcgaactg aacgtccaga cggtgtttag aaacgttgat gctcgggatt
     1681 ttttccgcac ccaggcaaac gcgcgccatg gctgcaacgg tttctgacgg atactgccca
     1741 cgg
//