From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human pre-mRNA splicing factor SF2p32, complete sequence.
Date: 29 Jul 92 10:03:07 GMT
Distribution: bionet
Checksum: 09264     4

LOCUS       HUMSF2P32    1240 bp ss-mRNA            PRI       29-JUL-1992
DEFINITION  Human pre-mRNA splicing factor SF2p32, complete sequence.
ACCESSION   M69039
KEYWORDS    SF2p32.
SOURCE      Homo sapiens Female cDNA to mRNA.
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 1240)
  AUTHORS   Krainer,A.R., Mayeda,A., Kozak,D. and Binns,G.
  TITLE     Functional expression of cloned human splicing factor sf2: Homology
            to RNA-binding proteins, U1 70K, and drosophila splicing regulators
  JOURNAL   Cell 66, 383-394 (1991)
  STANDARD  simple staff_entry
COMMENT     SF2p32 is a polypeptide from HeLa cells that copurifies with
            the essential pre-mRNA splicing factor SF2. It may be a subunit of
            SF2,
            although its function is presently unknown. This polypeptide has an
            apparent electrophoretic mobility of 32 kd, although its predicted
            molecular weight is 23,784 daltons. The HeLa protein has an
            unblocked
            N-terminus beginning with leucine.
            Purification of this protein from HeLa cells is described in
            Krainer, A.R.,
            Conway, G.C., and Kozak, D. (1990) Purification and
            Characterization of
            SF2, a Human Pre-mRNA Splicing Factor. Genes Dev. 4, 1158-1171.
FEATURES             Location/Qualifiers
     5'UTR           <1..207
     CDS             208..837
                     /codon_start=1
                     /translation="MHTDGDKAFVDFLSDEIKEERKIQKHKTLPKMSGGWELELNGTE
                     AKLVRKVAGEKITVTFNINNSIPPTFDGEEEPSQGQKVEEQEPELTSTPNFVVEVIKN
                     DDGKKALVLDCHYPEDEVGQEDEAESDIFSIREVSFQSTGESEWKDTNYTLNTDSLDW
                     ALYDHLMDFLADRGVDNTFADELVELSTALEHQEYITFLEDLKSFVKSQ"
     3'UTR           838..1226
     polyA_site      1227..1228
BASE COUNT      326 a    297 c    299 g    318 t
ORIGIN
        1 ctgcgctgcg tgccccgtgt gctgggctcc tccgtcgccg gcctccgcgc tgccgcgccc
       61 gcctcgcctt tccggcagct cctgcagccg gcaccccggc tgtgcacccg gcccttcggg
      121 ctgctcagcg tgcgcgcagg ttccgagcgg cggccgggcc tcctgcggcc tcgcggaccc
      181 tgcgcctgtg gctgtggctg cggctcgctg cacaccgacg gagacaaagc ttttgttgat
      241 ttcctgagtg atgaaattaa ggaggaaaga aaaattcaga agcataaaac cctccctaag
      301 atgtctggag gttgggagct ggaactgaat gggacagaag cgaaattagt gcggaaagtt
      361 gccggggaaa aaatcacggt cactttcaac attaacaaca gcatcccacc aacatttgat
      421 ggtgaggagg aaccctcgca agggcagaag gttgaagaac aggagcctga actgacatca
      481 actcccaatt tcgtggttga agttataaag aatgatgatg gcaagaaggc ccttgtgttg
      541 gactgtcatt atccagagga tgaggttgga caagaagacg aggctgagag tgacatcttc
      601 tctatcaggg aagttagctt tcagtccact ggcgagtctg aatggaagga tactaattat
      661 acactcaaca cagattcctt ggactgggcc ttatatgacc acctaatgga tttccttgcc
      721 gaccgagggg tggacaacac ttttgcagat gagctggtgg agctcagcac agccctggag
      781 caccaggagt acattacttt tcttgaagac ctcaagagtt ttgtcaagag ccagtagagc
      841 agacagatgc tgaaagccat agtttcatgg caggctttgg ccagtgaaca aatcctactc
      901 tgaagctaga catgtgcttt gaaatgatta tcatcctaat atcatggggg aaaaaatacc
      961 aaatttaaat tatatgtttt gtgttctcat ttattatcat ttttttctgt acaaatctat
     1021 tatttctaga tttttgtata acatgataga cataaaattg gtttatctcc tgctgagtct
     1081 tcctagaagt acctgctggg ttctcagttc cagttcccat cctttgattg atcactctcc
     1141 ctgacatcca cctgtatgac tttgtcacca aatgctgtct tctctttctc caatcaagaa
     1201 ataataatcc ctcgagttta caaaacaaaa aaaaaaaaaa
//