From: danj@welchgate.welch.jhu.edu (Dan Jacobson)
Subject: Re: looking for h2-Kd cDNA clone
Organization: Johns Hopkins Univ. Welch Medical Library
Date: Sun, 13 Dec 1992 23:24:34 GMT

In article <1992Dec13.140531.1@icrf.icnet.uk> p_travers@icrf.icnet.uk writes:
>Does anyone have an H2-Kd cDNA clone that they would be willing to send me?
>We are interested in peptide binding to MHC alleles and it turns out that Kd
>is best suited for our purposes
>
>			Paul Travers
>				
>			    Dept of Crystallography
>			    Birkbeck College, London
>			    P_TRAVERS@ICRF.ICNET.UK
> 

Below I have included the headers of three genes in Genbank which you
may find to be of interest - I would suggest that you look up the references 
given here and contact the authors as they may well have the clone that you're
looking for. If you would like to retrieve the full entries (ie these headers
and the sequence) you can do so via Gopher or e-mail server - if you don't
know how to do either drop me a note and I'll send you instructions.

If you look up the references and come up with a location for but not
the address of the authors - post a note to bionet.users.addresses ( or the
bionaut e-mail address) and the group will try and find an address for you.



Best of luck,


Dan Jacobson


danj@welchgate.welch.jhu.edu
 

============================================================================


LOCUS       MUSMHKD      1560 bp ss-mRNA            ROD       07-NOV-1984
DEFINITION  Mouse MHC class I H2-K gene (haplotype d), mRNA complete coding
            sequence, clone pH-2-d-33.
ACCESSION   J00402
KEYWORDS    antigen; histocompatibility antigen.
SOURCE      mouse (mus musculus), cDNA to mRNA, clone pH-2-d-33.
  ORGANISM  Mus musculus
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Rodentia; Myomorpha; Muridae; Murinae.
REFERENCE   1  (bases 1 to 1560)
  AUTHORS   Lalanne,J.-L., Delarbre,C., Gachelin,G. and Kourilsky,P.
  TITLE     a cdna clone containing the entire coding sequence of a mouse h-2kd
            histocompatibility antigen
  JOURNAL   Nucleic Acids Res. 11, 1567-1577 (1983)
  STANDARD  simple staff_review
COMMENT     submitted as clean copy from authors.
FEATURES             Location/Qualifiers
     CDS             26..1131
                     /note="MHC H2-K antigen"
                     /codon_start=1
//

 

LOCUS       MUSMHKDA     5141 bp ds-DNA             ROD       16-JUN-1986
DEFINITION  Mouse MHC class I H2-K gene (haplotype d), complete cds.
ACCESSION   X01815
KEYWORDS    antigen; class I antigen; histocompatibility antigen;
            membrane protein; polymorphism.
SOURCE      Mouse (DBA/2) liver DNA, clone lambda-2.14.
  ORGANISM  Mus musculus
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Rodentia; Myomorpha; Muridae; Murinae.
REFERENCE   1  (bases 1 to 5141)
  AUTHORS   Kvist,S., Roberts,L. and Dobberstein,B.
  TITLE     Mouse histocompatibility genes: Structure and organisation of a K-d
            gene
  JOURNAL   EMBO J. 2, 245-254 (1983)
  STANDARD  simple staff_review
COMMENT     EMBL features not translated to GenBank features:
               key        from     to       description

               PRM         160    164       CAAT-box
               PRM         187    190       TATA-box
               SITE       4460   4465       pot. polyA signal
               SITE       4475   4480       pot. polyA signal
//

 

LOCUS       MUSMHKDM     1111 bp ss-mRNA            ROD       07-NOV-1984
DEFINITION  Mouse MHC class I H2-K gene (haplotype d), mRNA, complete coding
            sequence, clone pH-2-d-24.
ACCESSION   K01182
KEYWORDS    antigen; cell surface glycoprotein; class I antigen; glycoprotein;
            histocompatibility antigen; major histocompatibility complex.
SOURCE      mouse (DBA/2 (H-2d haplotype)) cDNA to liver mRNA, clone pH-2d-24.
  ORGANISM  Mus musculus
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Rodentia; Myomorpha; Muridae; Murinae.
REFERENCE   1  (bases 1 to 1111)
  AUTHORS   Lalanne,J.-L., Cochet,M., Kummer,A.-M., Gachelin,G. and
            Kourilsky,P.
  TITLE     Different exon-intron organization at the 5' part of a mouse class
            I gene is used to generate a novel H-2Kd-related mRNA
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 80, 7561-7565 (1983)
  STANDARD  full staff_review
COMMENT     Clone pH-2d-24 codes for 24 polypeptide, which is almost identical
            to a genuine H-2Kd histocompatibility antigen. Clone pH-2d-24 was
            compared with clone pH-2d-33, also sequenced by the authors, and
            was found to be almost identical except at its 5' end. The authors
            suggest that this gene may encode two distinct products through an
            alternative use of splicing sites in the 5' region of the primary
            gene transcript.
//

 

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