From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: S. sanguis gene for StsI restriction endonuclease and StsI
Date: 31 Dec 92 01:40:25 GMT
Distribution: bionet
Checksum: 45403 10

LOCUS       STRSTSI      5183 bp ds-DNA             BCT       17-DEC-1992
DEFINITION  S. sanguis gene for StsI restriction endonuclease and StsI
            methylase, complete cds.
ACCESSION   D11101
KEYWORDS    StsI methylase; StsI restriction endonuclease.
SOURCE      Streptococcus sanguis (individual_isolate #54) genomic DNA, clone
            pSts627.
  ORGANISM  Streptococcus sanguis
            Prokaryotae; Firmicutes; Firmibacteria; Gram-positive cocci;
            Streptococcaceae.
REFERENCE   1  (bases 1 to 5183)
  AUTHORS   Kita,K., Suisha,M., Kotani,H., Yanase,H. and Kato,N.
  TITLE     Cloning and sequence analysis of the StsI restriction-modification
            gene: presence of homology to FokI restriction-modification enzymes
  JOURNAL   Nucleic Acids Res. 20, 4167-4172 (1992)
  STANDARD  full automatic
COMMENT     Submitted (11-MAY-1992) to DDBJ by: Keiko Kita Department of
            Biotechnology Tottori University 4-101 Koyama Tottori 680 Japan
            Phone:  0857-28-0321 Fax:    0857-31-0881.
FEATURES             Location/Qualifiers
     CDS             555..2363
                     /product="StsI restriction endonuclease"
                     /codon_start=1
                     /translation="MTISINEYSDLNNLAFGLGQDVSQDLKELVKVASIFMPDSKIHK
                     WLIDTRLEEVVTDLNLRYELKSVITNTPISVTWKQLTGTRTKREANSLVQAVFPGQCS
                     RLAIVDWAAKNYVSVAVAFGLLKFHRADKTFTISEIGIQAVKLYDSEELAELDKFLYE
                     RLLEYPYAAWLIRLLGNQPSKQFSKFDLGEHFGFIDELGFETAPIEIFLNGLAQAEID
                     GDKTAAQKIKSNFESTSDKYMRWLAGVLVTAGLATSTTKKVTHTYKNRKFELTLGTVY
                     QITAKGLTALKEVNGKSRYPRSRKRVMWEFLATKDKEAIAKKTSRSLMLKHLTEKKNP
                     IQAEVIATLINTDYPTLEITPEEVIDDCIGLNRIGIEILIDGDKLTLNDKLFDFEIPV
                     QKDVVLEKSDIEKFKNQLRTELTNIDHSYLKGIDIASKKKTSNVENTEFEAISTKIFT
                     DELGFSGKHLGGSNKPDGLLWDDDCAIILDSKAYSEGFPLTASHTDAMGRYLRQFTER
                     KEEIKPTWWDIAPEHLDNTYFAYVSGSFSGNYKEQLQKFRQDTNHLGGALEFVKLLLL
                     ANNYKTQKMSKKEVKKSILDYNISYEEYAPLLAEIE"
     CDS             complement(2489..4450)
                     /product="StsI methylase"
                     /codon_start=1
                     /translation="MRYIGSKKLLLPEIKKMVDKHTDGSEEVFLDLFAGTNVVANYFK
                     QFYTVYSNDMLFFSYVNAKATIENNSKPSFSKLIQAGISSPMTYLQNLEVNDETIGYY
                     EVAYSPTGEANYLSVHNAKKLDIIRSQIESWKNQNLLTEHEYYYLLSSLIEALPFISN
                     TTGTYGAFLKHWDKRSLNDLELQDFTIFDNSKQNKAFNEDANELVQKIKADIVYIDTP
                     YNSRQYASNYHLLENVARNEHPTLKGITKIFDWKNLKSDYATKGKALVAMRDLIQNIN
                     STHIILSYNNEGIISEEDLTNILKEFSVDGIVDIKKIPYRKYQSKNVSKNKEIYELLF
                     YIQRKPFSKNKTLNKPLNNVRVSSTKKYIKSPLNYIGGKYKLLNQILPLFPKNINTFV
                     DIFSGGANVGINVKAKKYIFNDMNTRINEMFRYFQTQPPVKLVQQIEEKIDEWGLSKT
                     NEDAFLAFRKHYNTNPNPLDLYVLSSFSYNYQFRFNNSMEFNNPFGRNRSHFSENMRN
                     NLLNFVTKLQTLDATFTDNYFNEFDFSNLTSNDFIYLDPPYLITTGSYNDGKRGFSDW
                     NNTSEMKLLNFMDYLNQHGIRFALSNVTEHKGKTNQLLKDWAYSRNLNVNYLDHNYNN
                     SSHNSKSKGSQEVLITNYETKTFNLLNTK"
BASE COUNT     1779 a    745 c    874 g   1785 t
ORIGIN      
        1 agtatatctg caaaatttaa aagattcatc cgcttacttt ttaatattat ccagttagag
       61 agggataaaa gctgaatcgg cgaacacctc tgtatttcga caataaaaca gccactgata
      121 gaagattttg ctaaaaatgg ctgtttttcc ttattttatc gttcagaatg agagtttaaa
      181 aaagaattga attattaact aaaaagataa ttatggtata ataggtacat ttaggaggtt
      241 cactatggtt gaatttactc ttgaaccttt ttctaacaac acttttaaat tacttaaatc
      301 attgaaagaa aaccaagttg aagtaaaagt catttattat ataccgctct ctcaacaaga
      361 gattgcaaac attaacaaaa tgtaaaactg aaaacgaata gacttctgcg tgatttgatt
      421 gaaggcggct ttgtctgccc ttatcaaaat aaacgtggaa aatatgaaat cactgaaaaa
      481 gtgcataagg tattgcgtct gattcagaag aaaaatatat agaaaaataa agaaataaaa
      541 atttaggagg aaatatgaca ataagcatta atgaatacag tgatttgaat aacttagcgt
      601 ttggtttagg gcaagatgtt agtcaggatt taaaagagtt agtaaaagtt gcttcaattt
      661 ttatgcctga ttcaaaaatt cataaatggt tgatagatac gagattagaa gaagtagtta
      721 cagatttaaa tcttcgatat gaattaaaat ctgtaattac taatacgcct atttcagtta
      781 cttggaaaca acttactgga acaagaacga agagagaagc taatagttta gttcaagcag
      841 tatttccagg acaatgttca aggcttgcaa ttgtagattg ggctgctaaa aattatgtga
      901 gtgtagcagt tgcttttgga ctattgaaat tccatcgggc agataaaact ttcacgattt
      961 cagagatagg aattcaagca gtaaagttat atgattctga agaattggca gaactggaca
     1021 aatttttata tgaaaggcta cttgagtatc cgtatgcagc gtggttgata agattattag
     1081 ggaatcaacc ctctaaacag ttttcaaaat ttgatttagg agaacatttt gggtttatag
     1141 atgaacttgg atttgaaaca gcccctatcg aaatattttt aaatggacta gcccaggctg
     1201 agattgatgg cgataaaaca gcagctcaaa aaatcaaatc aaacttcgaa agtacctctg
     1261 ataagtatat gagatggcta gctggtgttt tggttactgc aggattggca acttccacta
     1321 ctaaaaaagt tacccataca tacaaaaacc gtaaatttga acttactctt ggtacagtgt
     1381 atcagatcac agctaaagga ttaactgcct taaaggaggt taacggaaaa tcaagatatc
     1441 ctcgaagtag gaaaagagtg atgtgggaat tcttagcaac aaaggataaa gaagcaatcg
     1501 ctaaaaaaac atcacgatct ctaatgttaa aacaccttac tgaaaagaaa aatccaattc
     1561 aagcagaggt gattgctact ttaattaaca cagattatcc aactcttgaa ataacacctg
     1621 aagaagtgat agatgactgt attgggctta atagaattgg tattgaaatt ctgattgatg
     1681 gagataagct aacgttaaat gataaattgt ttgattttga aattcctgtg cagaaagatg
     1741 tggtattaga aaaaagtgat attgaaaaat ttaagaatca attgcgtacg gaactaacca
     1801 atattgacca ttcttatctt aaaggaattg atatagctag taaaaagaaa accagtaatg
     1861 ttgaaaatac ggaatttgaa gcaatatcaa ccaagatttt tacggatgag ttgggttttt
     1921 caggcaaaca tctaggagga agcaacaaac cagatggact cctgtgggat gatgattgtg
     1981 caattattct tgattcaaaa gcttactcag aaggctttcc actcactgcc tcccacacag
     2041 atgctatggg aagatatttg aggcaattta cagagcgaaa agaagaaata aagccaacgt
     2101 ggtgggatat tgctccagaa catttagaca atacatattt cgcttacgtt tctgggagtt
     2161 tttcgggtaa ttataaggaa cagttacaaa aatttaggca agatacaaac catttaggtg
     2221 gggcactaga gtttgttaaa ttgttattac tagcaaataa ttataaaact caaaaaatga
     2281 gtaaaaaaga agttaagaaa agtattcttg attataatat ttcatatgaa gaatatgctc
     2341 cattacttgc agaaatagag taagagaagt tatcgggata tggattgttt aaataataaa
     2401 agatgatttt cgttttgaaa agcatctttt ttcatcttgc ttgaagttat tatgtattga
     2461 ggttggtatg ggatagtatg tagttcttct attttgtatt aagcagatta aatgttttag
     2521 tttcataatt agttattaga acttcttgac tacctttgct ttttgaatta tgagacgaat
     2581 tgttataatt atgatcaaga taattgacat ttagatttct tgaataagcc caatctttta
     2641 acaattgatt tgttttacct ttgtgttcag ttacattact gagagcaaat cgaataccat
     2701 gttgatttag ataatccata aaatttagta acttcatttc agaagtatta ttccaatctg
     2761 aaaatcctcg ttttccatca ttataacttc cagttgtaat taaatatgga ggatcaagat
     2821 aaataaaatc attagatgtc aaattcgaaa aatcaaattc attaaaatag ttatctgtga
     2881 acgtggcatc taatgtttga agcttagtaa caaaatttaa taaattattt ctcatgtttt
     2941 cactaaagtg actacgattt ctaccaaaag gattattgaa ttccatagag ttattgaatc
     3001 taaattggta attgtaacta aatgaggata gaacatataa atctaaagga ttaggatttg
     3061 tattataatg tttcctgaaa gctaaaaatg catcttcatt agttttactc aatccccact
     3121 catcaatctt ttcttcgatt tgttgaacta atttgacagg tggttgagtt tgaaaatatc
     3181 gaaacatttc gttgatacga gtattcatat cattaaaaat atatttctta gctttaacat
     3241 ttatgcctac attagcgcct ccactaaata tatctacgaa cgtattaatg ttcttaggaa
     3301 aaaggggaag tatttgattt aatagtttat attttcctcc aatgtaatta agtggacttt
     3361 taatatattt ttttgttgaa ctgactctga cgttatttag aggtttgttc agagttttat
     3421 ttttagaaaa tggctttctt tggatataaa ataataattc ataaatttct ttgttcttag
     3481 aaacattctt tgattgatac tttcgatatg gaattttttt aatatcaact atgccatcaa
     3541 cagaaaattc tttcaaaata ttagtcaaat cctcttcgga gataatacct tcattattgt
     3601 aactcaaaat tatatgagtt gaatttatat tttgaattaa atctctcatt gctaccagag
     3661 ccttgccttt tgtagcgtaa tcacttttaa ggtttttcca atcgaaaatt tttgtaatac
     3721 cttttaaagt tgggtgttca tttctagcga cattttccag taaatgataa ttagaggcat
     3781 attgtcttga attataaggg gtatcaatat agacaatatc tgcctttatt ttttgaacta
     3841 gttcattcgc atcttcattg aatgctttgt tttgttttga gttatcaaat atagtaaaat
     3901 cttgtaattc taaatcattt aaagatcttt tatcccagtg ttttaggaaa gcgccataag
     3961 ttcctgtagt gttgctaata aatggcaatg cttctatgag agaagagagt agataataat
     4021 attcgtgttc tgttaataaa ttttgattct tccaactctc aatttgacta cgaattatat
     4081 ccagtttttt tgcattatgt actgaaaggt aatttgcctc tccagtgggc gaataagcaa
     4141 cttcgtagta tcctatcgtt tcatcgttga cttctaaatt ttgtaaataa gtcataggag
     4201 aactgatacc tgcctgaata agtttagaaa atgatggttt tgaattattt tcaattgtag
     4261 ctttagcatt gacatagcta aaaaataaca tgtcgttcga atagactgtg taaaattgtt
     4321 taaaataatt tgcaactaca tttgtaccag caaataaatc taagaacact tcctcagagc
     4381 catcagtatg tttatctacc atttttttaa tttcaggtag aagtaatttt tttgaaccaa
     4441 tatatctcat ttttacatcc tttatcatcc ttattttatc ataatagaag gaaaaatcca
     4501 actatgaata tatatttttc tgccttgata gtttaaatta cgcatgttgg cccactccaa
     4561 cacctgtaca ttctcctcat cgatcaatga aactctttcc tctaccaatt cctgatagag
     4621 aatatcttga atattcttct gatttaaaat tattgagact agcgaccacc tcgctagttt
     4681 tctatatcgg aggatatgac attctttggc aatggcatat agtctcgttt taggaagttc
     4741 aaggacctat tcacgtttgt taaaggaaag aacaccatct tcctcgttaa atttgaaata
     4801 agagctgtca acagcatctc ttgccttctc ggtttcttcc ttcaaaactt ccatgtaatg
     4861 ggaacttggt tttggtaata ctaattcttt ttgccttact tcagatgaag taaggttttt
     4921 ctttgtcttt aaaatctaaa taaagataga ttctgcatct ttttgagaag tgggataatc
     4981 ccatgtctgt tctccagtta cgaatatagg tgaaaaaacc aatatagtct tgtagatact
     5041 tagtggatac atttctcata tcacggtaga tatacttctt aatctcttta tggagtccat
     5101 ttactcaacc aagggataag ccattctggt atttagttcg gtgaaggtat caaatagcat
     5161 ttctccctat tggtaatatg atg
//