From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Chlorobium vibrioforme glutamyl-transfer RNA reductase (hemA) gene,
Date: 29 Dec 92 00:49:58 GMT
Distribution: bionet
Checksum: 60955 9

LOCUS       CHBHEMACD    3041 bp ds-DNA             BCT       04-DEC-1992
DEFINITION  Chlorobium vibrioforme glutamyl-transfer RNA reductase (hemA) gene,
            porphobilinogen deaminase (hemC) gene, uroporphyrinogen III
            cosynthase (hemD) gene, complete cds's.
ACCESSION   M96364
KEYWORDS    glutamyl-transfer RNA reductase; porphobilinogen deaminase;
            uroporphyrinogen III cosynthase.
SOURCE      Chlorobium vibrioforme (library: NCIB 8327) DNA.
  ORGANISM  Chlorobium vibrioforme
            Prokaryotae; Gracilicutes; Anoxyphotobacteria; Green bacteria;
            Green sulfur bacteria.
REFERENCE   1  (bases 1 to 3041)
  AUTHORS   Majumdar,D. and Wyche,J.H.
  TITLE     Hem a, c, d genes of the green sulfur bacterium, Chlorobium
            vibrioforme: Initial evidence of an operon
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     mRNA            52..3016
                     /function="heme/chlorophyll/siroheme/bilin/vitamin B12
                     biosynthesis"
                     /standard_name="glutamyl-tRNA reductase, PBG deaminase,
                     UROIII cosynthase"
                     /label=hemACD Operon
                     /citation=[1]
                     /note="A poly(A) signal, AATAAA, is present 20 nt
                     downstream of thehemA gene, 1320 to 1325. Shine-Dalgarno
                     sequences start at120,1583 and 2329, inside hemA, hemC and
                     hemD genes,respectively.; putative"
     polyA_site      1341..1342
                     /label=polyA-site
                     /citation=[1]
                     /note="putative"
     CDS             52..1299
                     /number=1
                     /function="synthesis of glutamate-1-semialdehyde"
                     /standard_name="glu-tRNA reductase"
                     /gene="hemA"
                     /label=hemA
                     /citation=[1]
                     /note="putative"
                     /product="glutamyl-transfer RNA reductase"
                     /codon_start=1
                     /translation="MNIISVGVNHKTAPIEIRERIALSEVQNKEFVTDLVSSGLASEA
                     MVVSTCNRTELYVVPGMPEVNCDYLKDYIISYKDARNAVRPEHFFNRFYCGTARHLFE
                     VSSAIDSLVLGEGQILGQVKDAYRIAAEVGTAGILLTRLCHSPFSVAKKVKTRTKLME
                     GAVSVSYAAVELAQKIFSNLSMKKVLLIGAVKQSWQQSTCTPRTPGTSSSPTGRNPRP
                     RACEELGTNRVLPYESYKEHLHEFDIIITAVSTKEYILNAAEMQQSMAKRRLKPVIIL
                     DLGLPRNVDPEVGALQNMFLKDIDALKHIIDKNLERRRAELPKVKSIIDEELIASASG
                     STPSRYVRPSLTCNPSSSKSRRKNSSVPPQGERRGVEAHGTPDRQDPEKNPASSYQDA
                     QGSGRYRRQHPQQSQPRQEHLRS"
     CDS             1437..2276
                     /number=2
                     /function="synthesis of hydroxymethylbilane"
                     /EC_number="4.3.1.8"
                     /standard_name="PBG deaminase"
                     /gene="hemC"
                     /label=hemC
                     /citation=[1]
                     /note="putative"
                     /product="porphobilinogen deaminase"
                     /codon_start=1
                     /translation="MNISLKLVKTTGDVLLDSPLSKIGDMGLFTKDIEKHLLAGEIDL
                     AVHSLKDVPTVRRKAWLSPRSPSVKTPRRHHFQVRQGVDGPSAEAKMATSSLRRMSQL
                     LSLRPDLEIMDIRGNLNTRFKKFDEGDFDAMMLAYAGVYRLEFSDRITEILPHETMLP
                     AVGQGALGIETRTDDAETREIVRVLNDDNTEMCCRAERALLRHLQGGCQIPIGSFGSY
                     IDGTLKLLAFVGSVDGKTGLRNEVTKAVKTPEEAEAVGIELAEILLSMGAEKILADIR
                     KTC"
     CDS             2276..3016
                     /number=3
                     /function="synthesis of uroporphyrinogenIII"
                     /EC_number="4.2.1.75"
                     /standard_name="UROIII cosynthase"
                     /gene="hemD"
                     /label=hemD
                     /citation=[1]
                     /note="putative"
                     /product="uroporphyrinogenIII cosynthase"
                     /codon_start=1
                     /translation="MKTVLVTRPKHQAAPFVRELEQYGLSTVVFPTIEIRPVAGWNVP
                     DLTKFAGIFFTSPNSVQFFLKHLLQTAPDELKNLQQTRVWAVGKTTGQDLEKHGVSIE
                     PLPKIADAVNLMADIDPAEIKGQTFLFVRGSLSLGTIPELIAEFGGTCVELTVYENLP
                     PSLEDTQRVKDMLAEGKLDCLSFTSPSTAKNFFEAIGSKELSDSVQIAAIGTTTSGAL
                     EKMGIKVDIIPEYFDGPSFAKAIAEALK"
BASE COUNT      800 a    866 c    757 g    618 t
ORIGIN      
        1 gatcaggctc tttttgcacg gtttacgaac ggttaacaaa ataatcctgc tatgaacatc
       61 atttcagttg gtgtcaacca caagacagca cctattgaaa tccgtgaaag gatcgctctt
      121 tcggaggttc agaacaagga gttcgtcacc gacctggtat cgagcggact ggcaagtgaa
      181 gccatggttg tatcgacctg caaccgtacg gaactctatg tggttccggg aatgcccgag
      241 gtgaactgcg attatctcaa agattacatc atctcataca aggacgcccg taacgccgtc
      301 cgtcccgaac acttcttcaa ccgcttctac tgcggcaccg cccgtcacct cttcgaggtt
      361 tccagcgcca tcgactctct ggtgctcggc gagggccaga ttctcggtca ggtcaaggac
      421 gcctaccgca tcgccgcgga ggtgggaact gccggcattc tgctcacaag actttgccac
      481 agccctttca gcgtcgcaaa aaaagtcaag actcgcacca aactcatgga gggcgccgtt
      541 tcggtcagct atgcagctgt cgaacttgcc cagaagatat tctcgaacct ctcgatgaag
      601 aaagtgctgc tgatcggcgc ggtgaaacag agctggcagc aaagcacatg tacgccaaga
      661 acgcccggaa catcgtcatc accaacagga cgcaatccaa ggccgagggc ttgcgaagag
      721 ctgggcacca accgcgtact tccttacgaa agctacaagg aacatctgca cgaattcgac
      781 atcatcatca cggcagtcag caccaaggag tatatcctca atgccgccga gatgcagcag
      841 agcatggcga aacgccgcct gaaaccggtc atcattctcg atctcggtct gccgagaaac
      901 gtcgatcccg aagtaggcgc actgcagaat atgttcctca aggatatcga cgcgctcaag
      961 catatcatcg acaaaaacct cgaacgccgc cgcgccgaac ttccgaaggt caagagcatt
     1021 atcgacgaag agctgatcgc ttcggccagt ggatcaacac cctcaaggta cgtccgacca
     1081 tcgttgacct gcaatccaag ttcatcgaaa tcaaggagaa agaactcgag cgtaccgcca
     1141 caaggtgagc gaagaggagt tgaagcgcat ggaacacctg accgacagga tcctgaaaaa
     1201 aatcctgcat catcctatca agatgctcaa ggctccggtc gataccgccg acaacatccc
     1261 cagcaaagtc aacctcgtca ggaacatctt cgatcttgaa gaaccaaacc agtcactcca
     1321 ataaaatcaa tctgtattcg cattgaaaaa agagattatc atcggcactc gttcagccgc
     1381 tggctctgtg gcaggctgag ttcaccaaag cagaactttc caagcgcttt cccgacatga
     1441 acatcagcct caagctggtc aaaacgaccg gtgacgtgct gctcgactct ccgctctcca
     1501 aaatcggaga tatgggtctg ttcaccaagg atatcgagaa acacctgctt gccggtgaga
     1561 tcgatctggc cgtgcacagc ctgaaggacg ttccgacggt acgccggaag gcctggttat
     1621 cacctcgttc accgagcgtg aagacaccgc gacgtcatca tttccaagtc cggcaagggg
     1681 ttgatggacc ttccgcagaa gccaaaatgg ctaccagctc cctgcgcaga atgtctcagc
     1741 tcctgagcct gaggcctgac ctggagatta tggacatccg cggcaacctc aacacccgct
     1801 tcaagaagtt cgacgaaggt gatttcgatg caatgatgct tgcttatgcc ggcgtctatc
     1861 gtcttgaatt cagcgaccgc atcaccgaaa tcctgccaca cgaaaccatg cttccggcag
     1921 ttggacaggg cgcactcggt atcgagaccc gcacggatga tgccgagacc agagaaattg
     1981 tccgcgtgct caacgatgac aacactgaaa tgtgctgccg cgccgaacgt gcgctgctca
     2041 ggcatttgca gggtggttgc cagatcccga tcggatcgtt cggctcgtac atcgacggca
     2101 ctctgaagtt gcttgcattt gttggttcgg tggatggcaa aaccggtctt cgcaacgaag
     2161 tgaccaaagc ggtcaagacc ccggaagaag cagaagccgt tggcatcgag ctggcagaga
     2221 tcctgctgtc aatgggcgca gaaaaaatcc ttgccgacat ccgcaaaacc tgctgatgaa
     2281 aacagttctc gttacacgcc caaaacatca ggcagcgcct tttgtcaggg agcttgagca
     2341 gtacggcctg agcacggttg tctttccgac aatcgagatc aggccggttg ccggctggaa
     2401 cgtacccgac ctcaccaaat tcgcgggcat ttttttcacc agccccaaca gcgtccagtt
     2461 tttcctcaaa catcttttgc agacagctcc tgacgagttg aaaaacctgc aacagacgcg
     2521 cgtgtgggct gtaggcaaaa ccacgggcca ggatctcgaa aaacatggcg tcagcattga
     2581 gccactgcct aaaatcgccg atgcggtcaa ccttatggcc gacattgatc cggctgaaat
     2641 caaaggccaa acattcctct ttgttcgcgg cagcctgtca cttggcacca ttccggaact
     2701 gatcgcagag ttcggtggaa cctgcgtcga gctgacagtt tatgaaaatc tgccaccctc
     2761 ccttgaagac acacagaggg tcaaggatat gcttgctgaa ggcaagctcg actgcctctc
     2821 cttcaccagt ccgtcgacgg ccaaaaactt cttcgaagca ataggctcga aagagctctc
     2881 tgactcggtt cagattgcag ccatcggcac cacaacttcc ggtgcgcttg aaaaaatggg
     2941 catcaaggtg gacatcattc ccgaatactt tgacggcccg agctttgcca aggcaatagc
     3001 tgaagctctg aaatgagttg caaaaaaaag aaattgtaag g
//