From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: D.pseudoobscura Adh gene for alcohol dehydrogenase
Date: 28 Dec 92 21:26:25 GMT
Distribution: bionet
Checksum: 44301 8

LOCUS       DROADHGN10   3485 bp ds-DNA             INV       01-DEC-1992
DEFINITION  D.pseudoobscura Adh gene for alcohol dehydrogenase
ACCESSION   X62190
KEYWORDS    ADH gene; alcohol dehydrogenase.
SOURCE      Drosophila pseudoobscura DNA.
  ORGANISM  Drosophila pseudoobscura
            Eukaryota; Animalia; Metazoa; Arthropoda; Uniramia; Insecta;
            Pterygota; Neoptera; Holometabola; Diptera; Brachycera;
            Cyclorrhapha; Schizophora; Drosophiloidea; Drosophilidae.
REFERENCE   1  (bases 1 to 3485)
  AUTHORS   Schaeffer,S.W. and Miller,E.L.
  TITLE     Estimates of gene flow in Drosophila pseudoobscura determined from
            nucleotide sequence analysis of the alcohol dehydrogenase region
  JOURNAL   Genetics 132, 471-480 (1992)
  STANDARD  full automatic
COMMENT     *source: strain=BR8F; *source: isolate=Bryce Canyon, Utah; *source:
            chromosome=IV section 88;
               From EMBL    entry DPADHGN10;  dated 16-OCT-1992.
FEATURES             Location/Qualifiers
     mRNA            join(40..119,915..1020,1084..1488,1549..1964)
                     /note="adult mRNA (Adh)"
     mRNA            join(882..1020,1084..1488,1549..1964)
                     /note="larval mRNA (Adh)"
     promoter        8..14
                     /note="adult promoter (Adh)"
     prim_transcript 40..1964
                     /note="adult primary transcript (Adh)"
     exon            40..119
                     /note="adult exon 1a"
     intron          120..914
                     /note="adult intron"
     promoter        846..853
                     /note="larval promoter (Adh)"
     prim_transcript 882..1964
                     /note="larval primary transcript (Adh)"
     exon            882..1020
                     /note="larval exon 1"
     exon            915..1020
                     /note="adult exon 1b"
     intron          1021..1083
                     /number=1
     exon            1084..1488
                     /number=2
     intron          1489..1548
                     /number=2
     exon            1549..1964
                     /number=3
     polyA_signal    1941..1946
     polyA_site      1964
     CDS             join(928..1020,1084..1488,1549..1815)
                     /EC_number="1.1.1.1"
                     /gene="Adh"
                     /product="alcoholdehydrogenase"
                     /codon_start=1
                     /translation="MSLTNKNVVFVAGLGGIGLDTSRELVKRNLKNLVILDRIDNPAA
                     IAELKAINPKVTITFYPYDVTVPVAETTKLLKTIFAQVKTIDVLINGAGILDDHQIER
                     TIAVNYTGLVNTTTAILDFWDKRKGGPGGIICNIGSVTGFNAIYQVPVYSGSKAAVVN
                     FTSSLAKLAPITGVTAYTVNPGITKTTLVHKFNSWLDVEPRVAEKLLEHPTQTSQQCA
                     ENFVKAIELNKNGAIWKLDLGTLEPITWTQHWDSGI"
BASE COUNT      970 a    794 c    796 g    925 t
ORIGIN      
        1 cgtagtgtat ttaaggcact gcacatgtcg aggaccacga ttattgtcta cagtgcagtc
       61 agtagttgtc agttgcagca ggcggaccag catttcttta aatctactca atcgatcaag
      121 taagtaactg taatccaagt acagtcggcc catcccactc ggttctgccg taactcaaga
      181 aatttcttga tagcgcagtt ccagttgtaa aacgtcgttt aaaaaaacat ctcgttagag
      241 cttgggttga aaaacgttcg agttgggcgt agaaaagttc aaatataaaa tatcaaaaaa
      301 gagaaaatat cttcccaaag cagctgtcca ttttttgtta aagtgataga tcaaaaattc
      361 gtttgacgta tttcccaagt gctctttatg caaataattt cctcaattct aattctacaa
      421 gaagcacttt tggctcttgt ccagaaaaaa caatttgttt acacttgtcg gttgataaca
      481 gaggcggacc gacggctaag taacataaca gtcgcctgtt aagtaatgcc ctcactctct
      541 gttagatggc cgcgcgtgtc gactccgata gaccagcaga cacgcagtag ttttcctcaa
      601 gaacctgaac tctaaacata gacataattt acgatacctt gagactcaaa taaaaataca
      661 agtatgagtg gtattctatt cctgattaga ctcgcacaca cacacgtacg agtattaaca
      721 taaatttgcc ggcaaataac caaaacaaaa cgaacgcaaa cgatccgaac acgtaatgcg
      781 agagataaga aacagaaaag ctcgacgtga aagaagcttc tggcggaagc tctttctgaa
      841 ctccctataa atacgagaca gaaaaccaga ataaatataa taacaaactg ttgaattgtc
      901 ccatcgattc ccagatcaaa agacagaatg tctctcacca acaagaacgt cgttttcgtg
      961 gccggtctgg gcggcattgg cctggacacc agccgggagt tggtcaagcg taatctgaag
     1021 gtaagagaga acgaattcca tggaatctat ggaatcctaa atttaaaaat acattttttt
     1081 tagaacctgg tcatcctgga tcgcattgac aatccggctg ccattgccga actgaaggca
     1141 atcaatccca aggtgaccat caccttctat ccctacgatg tgactgtgcc cgtcgctgag
     1201 accaccaagc tcctgaagac catctttgcc caggtgaaga caatcgatgt cctgatcaac
     1261 ggtgctggca tcctggacga tcatcagatc gagcgcacca ttgccgttaa ctacacgggc
     1321 ctggtcaaca ccaccacagc cattctggac ttctgggaca agcgcaaggg cggcccaggc
     1381 ggcatcattt gcaacattgg ctccgtcacc ggtttcaatg ccatctacca ggtgcccgtt
     1441 tactccggct ccaaggcggc ggtggttaac tttacctcct ccctggcggt aagtaatcca
     1501 acgtttttcc tactacccaa aactaaatat ttctttgaaa tcccatagaa actggctccc
     1561 attactggtg tcactgctta cactgtcaat cctggcatca ctaagaccac tctggtccac
     1621 aaattcaact cgtggctgga tgtggagccc cgtgtggcgg agaagctgct cgagcatccc
     1681 actcagacct ctcagcagtg cgccgagaac tttgtgaagg ccattgagct gaacaagaac
     1741 ggtgccatct ggaagttgga tctgggcacc ttggagccca tcacatggac ccagcactgg
     1801 gattcgggca tctaaaccat cccagagact ctatgggaca tggcgtttta gcttttagtt
     1861 tctttttttc cactcaattg ttacgtatat acatacacat atggcaataa ggctgatttg
     1921 aaccccgttt tgaatatgat aatacaaaat tatatttgag aaatttcaac aaaatcgata
     1981 agagctctag ctctctttta attaaaaaaa ctagataaaa agagcaatgg tcagtggtgg
     2041 tcagtggtcg ttgtggcctc tatcgatttc cacacaaaaa ctttactgtt tagtaataga
     2101 aaagaacttg agaaaggcag ccaaaagaat gtacgatctg acgggtaagc atgtctgcta
     2161 tgtggctgac tgcggtggca ttgcactgga gaccagcaag gttctcatga ccaagaatat
     2221 agcggtgagt ggtagagtgg tggtgtagag agtggagagt gttccgaagt ggttctggtt
     2281 cttaaacaag tctctagtct ctagtctcta gtctcttggc tgctgctggt agctgtcgaa
     2341 actaattaag ttaatgactt tttcatgttt tgtttttgtc cgctgacttt tgctgttcaa
     2401 agctacaatt aagtttatgt tttgagtttg atctactttt gcttaaggga agtactttcg
     2461 attacggatt gctggcatat aaaaatagat accacggaat gtgcatcata tcatattgat
     2521 tctatcttga ttctttgccc agaaactggc catccttcag agcgtggaaa accccccggc
     2581 catcgctcag ttacaatcga ttaagcacag cacacagatc ttcttctgga ccttcgatgt
     2641 gaccatggcc cgcgaggaga tgaagaagta cttcgatgag gtcatggtcc agatggacta
     2701 catcgatgtg ctgatcaatg gggccaccct gtgcgatgag cgtaacattg atgccaccat
     2761 caatacgaat ctgaccggca tgatgaacac cgtggccact gtgctgccct atatggaccg
     2821 aaagatgggc ggttcgggtg gactgattgt gaatgtcacc tccgtcatag gactggatcc
     2881 atcgccagtc ttttgtgcgt acagtgcctc aaagtttggt gtgattgggt tcacccgaag
     2941 tctagcggtg agtgggcgag atcttaactt gaaaatcctc tctaatgtct tccattcttt
     3001 cgcaggatcc cctgtattac acccaaaatg gtgtggctgt catggccgtc tgctgtggtc
     3061 ccaccaaagt gtttgtcgat cgggaactga ctgccttcct ggaatacggc cagtcctttg
     3121 ccgatcgcct gcgtcgtgcc ccctgccaat cgacggccgt ctgcggccag aatatagtaa
     3181 atgccatcga gagatcggag aacggacaga tctggattgc cgacaagggc ggcctggagt
     3241 cggtggccct ccactggtat tggcatatgg ccgatcagtt tgtgaactac atgcagagca
     3301 ctgacgatga ggatcaggag ttttttttgg gccagcgatg aggcatgcct tgatccacgg
     3361 aataaaacat caaatgtgtc acaaaaagta ggcaacttgt ttgtgggttt ggaaatagat
     3421 ttgaaatatt ttgaggtcat atgaataatg acataaatga gggtgcctga aagtatgcca
     3481 agttt
//