From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: S.pneumoniae (8250) ponA gene for penicillin-binding protein 1a
Date: 28 Dec 92 21:46:15 GMT
Distribution: bionet
Checksum: 30648 5

LOCUS       STRPONA6     1826 bp ds-DNA             BCT       02-DEC-1992
DEFINITION  S.pneumoniae (8250) ponA gene for penicillin-binding protein 1a
ACCESSION   X67871
KEYWORDS    penicillin-binding protein 1a; ponA gene.
SOURCE      Streptococcus pneumoniae DNA.
  ORGANISM  Streptococcus pneumoniae
            Prokaryotae; Firmicutes; Firmibacteria; Gram-positive cocci;
            Streptococcaceae.
REFERENCE   1  (bases 1 to 1826)
  AUTHORS   Martin,C., Sibold,C. and Hakenbeck,R.
  TITLE     Relatedness of penicillin-binding protein 1a genes from different
            clones of penicillin-resistant Streptococcus pneumoniae isolated in
            South Africa and Spain
  JOURNAL   EMBO J. 11, 3831-3836 (1992)
  STANDARD  full automatic
COMMENT     *source: strain=8250; *source: cell_line=plant cultivar; *source:
            clone=pcMK1-pcMK16;
               From EMBL    entry SPPONA6;  dated 21-OCT-1992.
FEATURES             Location/Qualifiers
     CDS             1..>1826
                     /gene="ponA"
                     /product="penicillin-binding protein1a"
                     /codon_start=1
                     /translation="MNKQTILRIAKYVSICFLTLFITAVMLGGGIFLYYVSKAPALSE
                     SKLVATTSSKIYDNKNQLIADLGSERRVNAQANDIPTDLVKAIVSIEDHRFFDHRGID
                     TIRILGAFLRNLQSNSLQGGSTLTQQLIKLTYFSTSTSDQTISRKAQEAWLAIQLEQK
                     ATKQEILTYYINKVYMSNGNYGMQTAAQNYYGKDLNNLSLPQLALLAGMPQAPNQYDP
                     YSHPEAAQDRRNLVLSEMKNQGYISAEQYEKAVNTPITDGLQSLKSASNYPAYMDNYL
                     KEVINQVEEETGYNLLTTGMDVYTNVDQEAQKHLWDIYNTDEYVAYPDDELQVASTIV
                     DVSNGKVIAQLGARHQSSNVSFGINQAVETNRDWGSTMKPITDYAPALEYGVYDSTAT
                     IVHDEPYNYPGTDTPVYNWDRGYFGNITLQYALQQSRNVPAVETLNKVGLNRAKTFLN
                     GLGIDYPSLHYSNAISSNTTESDKKYGASSEKMAAAYAAFANGGTYYKPMYIHKVVFS
                     DGSEKEFSNVGTRAMKEATAYMMTEMMKTVLTYGIGRGAYLPWLPQAGKTGTSNYTDE
                     EIEKYIKNTGYVAPDEMFVGYTRKYAMAVWTGYSNRLTPIIG"
BASE COUNT      582 a    422 c    364 g    458 t
ORIGIN      
        1 atgaacaaac aaactatcct gcgaatagct aagtatgtga gtatctgctt cttaacttta
       61 tttatcacag cagttatgct gggaggaggc atatttctct attacgtcag taaagcccca
      121 gccctatctg agagcaaatt agtcgcaacc acgtctagta aaatctacga caataaaaat
      181 caactcattg ctgacttggg ttctgaacgc cgcgtcaatg cccaagctaa tgatattccc
      241 acagatttgg ttaaggcaat cgtttctatc gaagaccatc gcttcttcga ccacaggggg
      301 attgatacca tccgtatcct gggagctttc ttgcgcaatc tgcaaagcaa ttccctccaa
      361 ggtggatcaa ctctcaccca acagttgatt aagttgactt acttttcaac ttcgacttcc
      421 gaccagacta tttctcgtaa ggctcaggaa gcttggttag cgattcagtt agaacaaaaa
      481 gcaaccaagc aagaaatctt gacctactat ataaataagg tctacatgtc taatgggaac
      541 tatggaatgc agacagcagc tcaaaactac tatggtaaag acctcaataa tttaagttta
      601 cctcagttag ccttgctggc tggaatgcct caggcaccaa accaatatga cccctattca
      661 catccagaag cagcccaaga ccgccgaaac ttggtcttat ctgaaatgaa aaatcaaggc
      721 tacatttctg ctgaacagta tgagaaagca gtcaatacac caattactga tggactacaa
      781 agtctcaaat cagcaagtaa ttaccctgct tacatggata attacctcaa ggaagtcatc
      841 aatcaagttg aagaagaaac aggctataac ctgctcacaa ctgggatgga tgtctacaca
      901 aatgtagacc aagaagctca aaaacatctg tgggatattt acaatacaga cgaatacgtt
      961 gcctatccag acgatgaatt gcaagttgct tctaccattg ttgatgtttc taacggtaaa
     1021 gtcattgccc agctaggagc acgccatcag tcaagtaatg tttccttcgg aattaaccaa
     1081 gcagtggaaa caaaccgcga ctggggatca actatgaaac ccatcacaga ttatgctcct
     1141 gccttggagt acggtgtcta cgattcaact gctactatcg ttcacgacga gccctataac
     1201 taccctggga cagatacccc tgtttataac tgggataggg gctactttgg caacatcacc
     1261 ttgcaatacg ccctgcaaca atcgcgaaac gtcccagccg tagaaactct aaacaaggtc
     1321 gggctcaacc gcgccaagac tttcctaaac ggtctcggaa tcgactaccc aagtcttcac
     1381 tactcaaatg ccatttcaag taacacaacc gagtcagata aaaaatatgg agcaagtagt
     1441 gaaaagatgg ctgctgctta cgctgccttt gcaaatggtg gaacttacta taaaccaatg
     1501 tatatccata aagtcgtctt tagtgatggg agtgaaaaag agttctctaa tgtcggaact
     1561 cgtgccatga aggaagcgac tgcttacatg atgacagaaa tgatgaaaac agtcttgacg
     1621 tacggaattg gtcgtggtgc ctacctgcct tggcttcctc aagctggtaa aacaggtacc
     1681 tctaactata cagatgaaga aattgaaaag tatatcaaga acactggtta cgtagctcca
     1741 gatgaaatgt ttgtagggta tacccgcaaa tatgcaatgg ctgtttggac aggatactca
     1801 aatcgtctaa ctccaatcat cggaga
//