From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: S.cerevisiae 8.2 kb segment left of MAT
Date: 15 Aug 92 10:03:37 GMT
Distribution: bionet
Checksum: 10385    17

LOCUS       YSCSMAT      8280 bp ds-DNA             PLN       15-AUG-1992
DEFINITION  S.cerevisiae 8.2 kb segment left of MAT
ACCESSION   X56909
KEYWORDS    ORF; ribokinase.
SOURCE      Saccharomyces cerevisiae DNA.
  ORGANISM  Saccharomyces cerevisiae
            Eukaryota; Fungi; Eumycota; Hemiascomycetes; Endomycetales;
            Saccharomycetaceae.
REFERENCE   1  (bases 1 to 8280)
  AUTHORS   Thierry,A., Fairhead,C. and Dujon,B.
  TITLE     The Complete Sequence of the 8.2kb Segment Left of MAT on
            Chromosome III Reveals Five ORFs, Including a Gene for a Yeast
            Ribokinase
  JOURNAL   Yeast 6, 521-534 (1990)
  STANDARD  full automatic
COMMENT     *source: strain=FY1679; *source: haplotype=diploid; *source:
            chromosome=III;
            From EMBL    entry SCSMAT;  dated 05-JUN-1992.
FEATURES             Location/Qualifiers
     TATA_signal     115..119
                     /note="YCR521"
     CDS             179..1222
                     /gene="YCR521"
                     /codon_start=1
                     /translation="MNSLVTQYAAPLFERYPQLHDYLPTLERPFFNISLWEHFDDVVT
                     RVTNGRFVPSEFQFIAGELPLSTLPPVLYAITAYYVIIFGGRFLLSKSKPFKLNGLFQ
                     LHNLVLTSLSLTLLLLMVEQLVPIIVQHGLYFAICNIGAWTQPLVTLYYMNYIVKFIE
                     FIDTFFLVLKHKKLTFLHTYHHGATALLCYTQLMGTTSISWVPISLNLGVHVVMYWYY
                     FLAARGIRVWWKEWVTRFQIIQFVLDIGFIYFAVYQKAVHLYFPILPHCGDCVGSTTA
                     TFAGCAIISSYLVLFISFYINVYKRKGTKTSRVVKRAHGGVAAKVNEYVNVDLKNVPT
                     PSPSPKPQHRRKR"
     polyA_signal    complement(1307..1311)
                     /note="YCR522"
     CDS             complement(1421..2605)
                     /gene="YCR522"
                     /codon_start=1
                     /translation="MAESTTLETIEIHPITFPPEVLARISPELSLQRHLSLGIRPCLR
                     KYEEFRDVAIENNTLSRYADAGNIDTKNNILGSNVLKSGKTIVITSITGGIIEETSAS
                     IKDLDDFGEEELFEVTKEEDIIANYASVYPVVEVERGRVGACTDEEMTISQKLHDSIL
                     HSRILPKKALKVKAGVRSANEDGTFSVLYPDELEDDTLNETNLKMKRKWSYVLYAKIV
                     VLSRTGPVFDLCWNSLMYALQSVKLPRAFIDERASDLRMTIRTRGRSATIRETYEIIC
                     DQTKSVPLMINAKNIAFASNYGIVELDPECQLQNSDNSEEEEVDIDMDKLNTVLIADL
                     DTEAEETSIHSTISILAAPSGNYKQLTLMGGGAKITPEMIKRSLLLSRVRADDLSTRF
                     NI"
     TATA_signal     complement(2685..2689)
                     /note="YCR522"
     misc_feature    complement(2701..2717)
                     /note="CT block upstream of YCR522"
     TATA_signal     2720..2724
                     /note="YCR523"
     TATA_signal     2779..2783
                     /note="YCR523"
     CDS             2884..3885
                     /product="ribokinase"
                     /gene="YCR523"
                     /codon_start=1
                     /translation="MGITVIGSLNYDLDTFTDRLPNAGETFRANHFETHAGGKGLNQA
                     AAIGKLKNPSSRYSVRMIGNVGNDTFGKQLKDTLSDCGVDITHVGTYEGINTGTATIL
                     IEEKAGGQNRILIVEGANSKTIYDPKQLCEIFPEGKEEEEYVVFQHEIPDPLSIIKWI
                     HANRPNFQIVYNPSPFKTMPKKDWELVDLLVVNEIEGLQIVESVFDNELVEEIREKIK
                     DDFLGEYRKICELLYEKLMNRKKRGIVVMTLGSRGVLFCSHESPEVQFLPAIQNVSVV
                     DTTGAGDTFLGGLVTQLYQGETLSMAIKFSTLASSLTIQRKGAAESMPLYKDVQKDA"
     polyA_signal    3972..3976
                     /note="YCR523"
     CDS             complement(4001..6772)
                     /gene="YCR524"
                     /codon_start=1
                     /translation="MRFSHFLKYNAVPEWQNHYLDYNELKNLIYTLQTDELKQETPTG
                     DLNDDADSQTPGPIADIESNIAAGEPSPSKRRFTHKLKRKLFGSKTPSGSKRGDSDEK
                     AIDGNNINEETIELDELSPQGKTTSFNKNFIRKKFFESRSSSVSSEGKTLFSSYDTFV
                     TNLSDEKLKVDDFYKRMEAKFYERFDHLINDLEKEGIVTRLNETFNPEIQALPPLREI
                     ISGTSETHSSNNPFEIHSSNIDSELRNRFDYSEEEMDEDDDVDVFADTTDNTALLNYS
                     QFNIKSQKKSLLKQTIINLYIDLCQLKSFIELNRMGFSKITKKSDKVLHMNTRQELIE
                     SEEFFKDTYIFQHETLSSLNSKIAQLIEFYAVLMGQPGNVDSCKQELKSYLHDHIVWE
                     RSNTWKDMLGLSSQNNDIITIEDEAEKLMQEKLQIEYFKYPLPKPINLKFTKIENLAV
                     PKLFFGKRAMKIGFIIIVTGVLLGVKTFNDPVEHRCMALVECCAFLWASEAIPLHITG
                     LLVPLLTVLFRVLKDDDGKVMGAAAASTEILGTMWSSTIMILLAGFTLGEALSQYNVA
                     KVLASWLLALAGTKPRNVLLMAMSVVFFLSMWISNVASPVLTYSLLTPLLDPLDYTSP
                     FAKALVMGVALSADIGGMASPISSPQNIISMQYLKPYGIGWGQFFAVALPTGILSMLC
                     SWALMILTFKIGKTKLEKFKPIRTRFTIKQYFIIIVTIATILLWCVESQIESAFGSSG
                     EIAVIPIVLFFGTGLLSTKDFNTFPWSIVVLAMGGIALGKAVSSSGLLVTIARALQKK
                     IQNDGVFAILCIFGILMLVVGTFVSHTVSAIIIIPLVQEVGDKLSDPKAAPILVFGCA
                     LLASCGMGLASSGFPNVTAISMTDKKGNRWLTVGAFISRGVPASLLAFVCVITLGYGI
                     SSSVLKGST"
     TATA_signal     complement(6785..6789)
                     /note="YCR524"
     TATA_signal     complement(6866..6870)
                     /note="YCR524"
     misc_feature    complement(6928..6958)
                     /note="CT block upstream of YCR524"
     polyA_signal    complement(7150..7154)
                     /note="YCR526"
     CDS             complement(7208..8280)
                     /partial
                     /gene="YCR526"
                     /codon_start=3
                     /translation="SVPRLIQIWVESYWQDSETTLKDILNFWYSHLAEYYEYQELFAD
                     IVQLFINKKRTRQLKIHYIGLTDKEIEENKPPLDYENLFLQYEIDKTNANDELCGATD
                     LSDLLFQWKQGEPLEVEAFALNVSPWSLAKTLTLLESSLYLDIETIEFTRHFKHNDTT
                     IDSVFTLSNQLSSYVLETTLQQTHTISYWLQVALACLYLRNLNSLASIITSLQNHSIE
                     RLSLPIDVKSDHLFQRLKVVVHPNNNYNVYRRTIKHIFHSQLPCVPFTSLLIRDITFI
                     RDGNDTFTKDGNNVNMQKFNQITKIVAFAQYLQQKQYEDIHCSNTTARSLLGAMIKVH
                     TLYNDNKDRAYQVSIAKVPRLT"
                     /partial
     misc_feature    8258
                     /note="MAT W junction"
BASE COUNT     2455 a   1537 c   1561 g   2727 t
ORIGIN
        1 tttgtaagta ttcacatgtc ctggcgtgcg gcattgctga aaatcgcaaa acccacagag
       61 aaataaacat cgcgaaaaag tcaatgaaaa attggaaaat atttttcatt tcactattat
      121 ccacaagcaa ttttgtacaa agtgaaaagg ttgaactaat tatcttcgtc tagaagccat
      181 gaattcactc gttactcaat atgctgctcc gttgttcgag cgttatcccc aacttcatga
      241 ctatttacca actttggagc gaccattttt taatatttcg ttgtgggaac atttcgatga
      301 tgtcgtcact cgtgtaacta acggtagatt tgttccaagc gaattccaat tcattgcagg
      361 tgaattacca ttaagcactt tgccccctgt gctatacgcc atcactgcct attacgttat
      421 tatttttggt ggcaggtttt tgttaagtaa gtcgaaacca tttaaattaa atggcctttt
      481 ccaattgcat aatttggttt taacttcact ttcattgacg cttttattgc ttatggttga
      541 acaattagtg ccaattattg ttcagcacgg gttatacttc gctatctgta atattggtgc
      601 ttggactcaa ccgctcgtta cattatatta catgaattac attgtcaagt ttattgaatt
      661 tatagacacc tttttcttgg tgctaaaaca taaaaaattg acatttttgc atacttatca
      721 ccatggcgct actgccttat tatgttacac ccaattgatg ggcaccacat ctatttcttg
      781 ggtccctatt tcattgaacc ttggtgttca cgtggttatg tattggtact atttcttggc
      841 tgccagaggc atcagggtct ggtggaagga atgggttacc agatttcaaa ttatccaatt
      901 tgttttggat atcggtttca tatattttgc tgtctaccaa aaagcagttc acttgtattt
      961 cccaattttg ccacattgtg gtgactgtgt gggttcaaca actgccacct ttgcaggttg
     1021 tgccattatt tcttcatatt tggtactatt tatttcattt tacattaacg tttataaacg
     1081 taaaggcacc aaaaccagta gagtggtaaa gcgtgcccac ggcggtgttg ccgcaaaggt
     1141 taatgagtat gttaacgttg acttgaaaaa cgttcctact ccatctccat caccaaaacc
     1201 tcaacacaga agaaaaaggt aagtgtaaaa tctttgaaag aatttaagta ttcaactttc
     1261 gtatattcgt tttttcttag tggatctatt gttactatta tcactattat tatattgtaa
     1321 aagaccggat ggttttgtta tatattacat acacatgtta tcgttgaaaa aagttttccg
     1381 tttcctttcg acagtcatca gataatttta tccgagtctt ttatatgtta aatcttgttg
     1441 acaaatcgtc cgctctaacc ctagataaca acaatgatct ttttatcatt tctggcgtta
     1501 ttttagcgcc tcctcccatt aatgttaatt gcttatagtt acctgaagga gcagcgagga
     1561 tggagatcgt actgtgtata cttgtttctt ccgcttcagt atctaggtct gctattagca
     1621 cagtgttcaa tttatccata tcaatgtcca cttcctcttc ttcagagtta tcagagtttt
     1681 gtaattggca ttcggggtct aactccacta tcccataatt tgaagcaaat gcaatattct
     1741 ttgcgtttat cattaacggt actgacttag tttggtcgca aataatttca tacgtttctc
     1801 ttatggtggc acttcttcct cttgttctta tagtcattct taaatcggac gcacgctcat
     1861 ctataaatgc tctaggtaac tttacgctct gtaaagcgta catcaaagaa ttccaacaca
     1921 aatcgaagac tggaccagtg cgactcaaga ctacaatttt cgcatacaaa acgtatgacc
     1981 attttctttt cattttcagg tttgtttcat tcagcgtatc atcttctaat tcatcgggat
     2041 acaagacgga aaacgtaccg tcttcattcg cactacgaac accagctttt accttcaagg
     2101 cttttttagg caatatccta gagtgcaata tggaatcgtg tagtttttgt gatatagtca
     2161 tttcttcatc ggtgcaagcg cccactctac ctctttcaac ttccaccacc ggatagacag
     2221 aagcataatt tgcaattatg tcctcctctt tggtcacttc gaacaattct tcctcaccga
     2281 aatcatccaa atctttaatc gaggcgctag tttcttctat tattccaccc gtaatagaag
     2341 ttatgactat ggttttccca cttttcaaaa catttgagcc taaaatgttg tttttagtgt
     2401 ctatattacc agcatccgca taacgcgata aagtattatt ttctatagca acatctctaa
     2461 attcttcata ttttcttaga catggcctga ttcctaggga taagtgtctt tgtaaagaca
     2521 attctggaga tattctcgca agaacttcag gtggaaaagt aattgggtgt atctcaatag
     2581 tttctaacgt ggtactttca gccatttcca atattagatg cctgtgaaac tactgtcaaa
     2641 cttatttgtg tagcaatact catcgtctaa tttgtccatt attgttatat actatgtcat
     2701 aaaaaaagaa agaaaaatct tatactttcg tcgactgtga tttgaaaatc tctccagatc
     2761 aaacgggaat aaaagggttt atatccgaag gtggccagaa caatcatgaa gcaaatagga
     2821 taaaaatagc caataagatg tttgttgtct ttcatcccaa agttccaaca gcatatttca
     2881 aagatgggta ttacagtaat aggttctcta aactatgatt tggacacatt tacggataga
     2941 ttacctaacg ctggagaaac tttcagggct aaccacttcg aaacacatgc tggtggtaag
     3001 ggattgaacc aagctgcggc cattggtaaa ttaaaaaacc ccagcagcag atatagtgtt
     3061 cgaatgattg gtaatgttgg aaatgataca tttggtaaac aattgaagga cactttatcc
     3121 gattgcggag tcgatatcac tcacgtcggt acttacgaag gcattaatac gggtaccgct
     3181 accatattaa ttgaagagaa agctggtggc caaaatagga tattgattgt agaaggtgct
     3241 aacagcaaga ctatttatga cccgaaacag ttgtgtgaaa tttttccaga gggcaaggag
     3301 gaagaagagt atgttgtttt tcaacacgaa attcctgatc ctctttccat tattaaatgg
     3361 atacatgcga acaggccgaa ttttcagatc gtatataacc cctcaccttt caagaccatg
     3421 cctaagaaag attgggagtt ggtagacctt ttggtcgtta atgaaattga gggtcttcaa
     3481 atcgtggaaa gtgtatttga taatgaactt gttgaagaaa taagggagaa gataaaggac
     3541 gactttttag gagaatatcg taaaatttgt gagcttttgt atgaaaaact catgaatcga
     3601 aagaaaagag gaattgtggt tatgactttg ggttcgagag gggtgctttt ctgttcgcac
     3661 gaaagccctg aagtacaatt ccttccggct attcaaaatg tttcggttgt tgatactaca
     3721 ggagctggag atactttcct gggcggtttg gttactcaat tgtatcaagg agagaccttg
     3781 tctatggcta taaagttctc tacattagct agttcattga ccattcaaag aaaaggtgct
     3841 gctgaaagca tgccactgta taaagatgtt cagaaagatg cataaaatct actgcaattt
     3901 tttactttta gtttgttaaa ttttagtttt cgtcatatat ataaagttta aaaaggtaca
     3961 aagaatgcag taataagaaa tgtaattaca tgagggcagt ttaagtgcta ccttttaaga
     4021 cggaagaact aataccataa ccgagagtaa ttacgcagac aaacgctaac aacgaagcag
     4081 gaacacctct ggagataaaa gcgcctacag ttagccatct attacccttt ttatcggtca
     4141 tagaaatagc agtaacgttt ggaaatccag atgaagccaa ccccataccg catgaggcta
     4201 acaaggcgca accgaacaca agaattggag ctgcctttgg atcggataat ttgtcaccaa
     4261 cttcttgcac caagggaata atgatgattg ctgacacagt atgtgagaca aaagtgccca
     4321 caactaacat taaaataccg aaaatacata agatagcaaa aacaccatcg ttctgaattt
     4381 tcttttgtaa tgctcttgca atagttacca acaagcctga agatgaaact gccttaccaa
     4441 gggctatacc acccatagca agaacaacaa ttgaccaagg gaatgtgttg aaatcctttg
     4501 ttgatagtag acctgtacca aaaaacagga ctatcggtat tactgcaatt tcaccggacg
     4561 atccaaaagc actttctatt tgtgactcta cacaccatag aagaatagta gcaatagtta
     4621 caatgatgat aaaatattgc tttatagtaa atctggtcct tattggttta aatttttcca
     4681 gtttagtttt gcctatttta aaggtgagta tcatcaaggc ccaggagcac agcatcgata
     4741 gaatacctgt aggcagagcg acagcaaaaa attgccccca gccgattcca taaggtttta
     4801 agtactgcat ggagatgata ttctgtggcg aagaaatagg tgaagccata ccaccaatat
     4861 ctgccgaaag tgcaacaccc atgactaatg ccttagcaaa cggtgaagtg tagtccagcg
     4921 gatctagtaa gggtgttaat agagaatatg tcaatactgg ggaggcaacg ttggaaatcc
     4981 acatcgaaag aaagaataca acactcattg ccattaaaag gacatttctt ggcttggtac
     5041 ctgcaagggc caataaccac gatgccaaaa ctttcgcaac gttatattgc gacaaggctt
     5101 cacccaatgt gaaacctgct aataaaatca taattgttga cgaccacatt gtacctaaga
     5161 tttctgtaga ggcagctgct gctcccatta ccttaccgtc atcgtctttt agtaccctaa
     5221 aaaggacagt tagaagggga accaataaac ctgtgatgtg taatggaatg gcttcactag
     5281 cccataagaa agcacagcat tctaccaatg ccatacaccg gtgttcgaca gggtcattga
     5341 aagttttaac acccaacaaa acacctgtga caatgataat gaagcctatt ttcattgctc
     5401 ttttcccaaa aaatagctta ggaactgcca aattttcaat tttagtaaac ttcaaattaa
     5461 ttggcttagg caatggatac ttgaaatatt caatttgaag cttttcttgc ataagtttct
     5521 cagcttcatc ttcaatagtt attatatcgt tattttgcga agagaggccc aacatgtctt
     5581 tccatgtgtt gcttctttcc caaacaatgt ggtcgtgcag gtacgacttt aactcttgct
     5641 tgcatgaatc tacgttccca ggctgaccca tgagaacagc ataaaattca ataagttgtg
     5701 caattttact gtttaaactg cttaaagttt catgctggaa gatgtaggtg tctttgaaaa
     5761 attcttcact ttctattaat tcttgcctag tgttcatgtg caatacttta tcagacttct
     5821 tagtaatttt actgaaaccc attctgttca attcgataaa agatttcaac tggcaaaggt
     5881 ctatgtaaag atttattatt gtctgtttta ataatgattt tttctgagat ttaatgttaa
     5941 attgcgaata attcaagagg gcggtattgt cggtagtgtc agcaaacacg tcaacgtcat
     6001 catcttcatc catttcttct tcgctgtaat caaacctatt tctcaattca ctgtcgatgt
     6061 ttgaagagtg tatttcaaat gggttattag atgaatgtgt ctctgatgta ccagaaataa
     6121 tttctcttaa aggaggcaat gcttgaattt caggattgaa agtttcattc aatcttgtta
     6181 caatgccttc cttctccaaa tcattaatca agtggtcaaa tctttcatag aacttagctt
     6241 ccattctttt gtagaaatca tctactttca atttctcgtc gctcaggtta gttacgaatg
     6301 tatcataaga actgaagagc gtctttccct cgctactcac agatgagctg cgtgattcaa
     6361 agaatttctt acgtataaaa ttcttattga aagaggtggt tttcccttga ggagataact
     6421 cgtctaactc aattgtttcc tcgttaatat tgttcccatc tatggccttt tcgtcggagt
     6481 ctcccctttt gcttcctgaa ggtgttttag aaccaaagag cttacgcttg agtttatgtg
     6541 taaatcttct tttcgatgga gatggttctc ctgcagctat gttgctttct atatcagcga
     6601 ttggacctgg agtctgagag tcagcgtcat cgtttaagtc accggttggc gtttcttgtt
     6661 tcaattcatc tgtctgtaat gtgtagatca aatttttcaa ttcgttataa tctaggtaat
     6721 gattctgcca ttcagggaca gcgttgtatt tgagaaagtg tgagaatctc atttcgaggc
     6781 acaataatag ccgcctttac cgtagttttg ctgcaccttt atctgagagc tgactgcttt
     6841 tttggtgtga aacactgttt tctggtaata atttttcaat gcatcggatt acctttccca
     6901 cgtgcgaaat catcaattaa ttagattgaa aaaagggtaa gggaaaataa gaaagaggcg
     6961 gtagcctaaa gatacggtaa ttgaaacgtt tcctatgcac aatcttaaac ctttttaggt
     7021 aattgattaa gttgactgta atatctgtaa aagattacat ctaatttacg tctggtttct
     7081 tatcaaaaaa tattatctac tgtagttatg caagtattct taactttcag tactaaacat
     7141 gaatattaat tatttgtgag cgtacaagaa gcaaaaggaa gtcatctttc tttgaacagt
     7201 tctgttttta ggtaagcctt ggaaccttag ctatactgac ttgatacgcc ctgtctttgt
     7261 tgtcgttata taaagtgtgc acctttatca tagcccctaa taagcttctt gcagtagtat
     7321 ttgaacagtg tatatcttca tattgctttt gttgtaaata ttgcgcaaaa gcgactatct
     7381 ttgtgatttg gttgaacttt tgcatattca cgttattacc atctttagtg aatgtatcgt
     7441 ttccgtctct tatgaaggta atgtccctga taagcagtga tgtaaaaggt acacaaggaa
     7501 gctgactgtg gaaaatatgt ttaattgttc ttctataaac gttgtagttg ttgtttggat
     7561 gtacgacgac ttttaggcgc tgaaaaaggt ggtctgattt aacatctatc gggagagata
     7621 gtctttctat tgaatgattt tgcaatgatg taatgattga agcaagtgag tttaagtttc
     7681 gtaagtatag acaagcaagt gcaacttgta accagtagga aatggtgtgc gtttgctgca
     7741 aagttgtctc aagaacgtag gacgataact ggttggaaag cgtaaacacg gagtcaattg
     7801 ttgtatcgtt gtgtttgaaa tgtcttgtga attctattgt ttcaatatcc aagtaaagag
     7861 aagattctaa gagagtcaat gtctttgcaa gtgaccatgg agatacgttt agagcgaagg
     7921 cttcgacttc tagaggttca ccctgtttcc attggaaaag taaatcactg aggtcagttg
     7981 caccgcacaa ttcatcattt gcgttcgttt tgtctatctc gtattggaga aataagtttt
     8041 cgtagtccag gggcggttta ttttcttcga tttccttatc agttagacca atgtaatgaa
     8101 tcttcaattg cctcgttctt tttttgttta taaacagctg aactatgtct gcaaacagtt
     8161 cttggtattc ataatattca gccaagtgac tgtaccaaaa attgaggata tcttttaatg
     8221 ttgtctcact atcttgccaa taagactcta cccagatttg tattagacga gggacggagt
//