From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: E.coli MsbA gene
Date: 21 Jul 92 10:04:19 GMT
Article-I.D.: genbank.Jul.21.03.04.18.1992.18030
Distribution: bionet
Checksum: 27462     8

LOCUS       ECOMSBAG     3468 bp ds-DNA             BCT       21-JUL-1992
DEFINITION  E.coli MsbA gene
ACCESSION   Z11796
KEYWORDS    MsbA gene; OrfE; suppressor.
SOURCE      Escherichia coli DNA.
  ORGANISM  Escherichia coli
            Prokaryotae; Gram-negative facultatively anaerobic rods;
            Enterobacteriaceae.
REFERENCE   1  (bases 1 to 3468)
  AUTHORS   Karow,M.L. and Georgopoulos,C.P.
  TITLE     The essential Escherichia coli msbA gene, a multicopy suppressor of
            htrB, is related to the family of ATP-dependent translocators
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
REFERENCE   2  (bases 1 to 3468)
  AUTHORS   Karow,M.L.
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
COMMENT     *source: clone_library=mini-Mu, pMu d5005; *source: clone=pMM4;
            *source: is_macronuclear=N; *source: is_proviral=N; *source:
            is_germline=N;
            From EMBL    entry ECMSBAG;  dated 09-JUN-1992.
FEATURES             Location/Qualifiers
     CDS             562..2310
                     /note="member of ATP-dependenttransport family, very
                     similar to mdr proteins andhemolysin B, export protein"
                     /gene="MsbA"
                     /codon_start=1
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DSLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     CDS             2307..3293
                     /function="unknown"
                     /note="essential gene product"
                     /gene="OrfE"
                     /codon_start=1
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARNVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTLLASGN"
BASE COUNT      785 a    835 c    960 g    888 t
ORIGIN
        1 tccgttaaac tgggaacatc atcagccctg tgtgcgtggc gaacgtggca atggcaagga
       61 ttgcgtttca gcgcgcactg gcctttacaa ggtagcaacg ataaaggaaa taaccattcc
      121 tgtgtggtta aggttgatga cgggacgaat agcattcttc taaccggtga tattgaagcc
      181 ccagctgaac aaaagatgct aagccgttac tggcagcaag tgcaggcaac attgcttcag
      241 gtacctcacc atggcagtaa tacctcatca tcgttgccat taattcagcg agtgaatgga
      301 aaagtggcac tcgcatcggc atcgcgctat aacgcatggc gactgccctc taacaaagtt
      361 aagcatcgct atcaactgca aggatatcaa tggattgata ctccacatca aggtcaaaca
      421 acggtcaatt tttcagcgca aggctggcgg attagcagcc tcagggagca aattttacct
      481 cgttggtatc atcagtggtt tggcgtgcca gtggataacg ggtagaatat gcggctattt
      541 caacaaatgc tggttttttg aatgcataac gacaaagatc tctctacgtg gcagacattc
      601 cgccgactgt ggccaaccat tgcgcctttc aaagcgggtc tgatcgtggc gggcgtagcg
      661 ttaatcctca acgcagccag cgataccttc atgttatcgc tccttaagcc acttcttgat
      721 gatggctttg gtaaaacaga tcgctccgtg ctggtgtgga tgccgctggt ggtgatcggg
      781 ctgatgattt tacgtggtat caccagctat gtctccagct actgtatctc ctgggtatca
      841 ggaaaggtgg taatgaccat gcgtcgccgc ctgtttggtc acatgatggg aatgccagtt
      901 tcattctttg acaaacagtc aacgggtacg ctgttgtcac gtattaccta cgattccgaa
      961 caggttgctt cttcttcttc cggcgcactg attactgttg tgcgtgaagg tgcgtcgatc
     1021 atcggcctgt tcatcatgat gttctattac agttggcaac tgtcgatcat tttgattgtg
     1081 ctggcaccga ttgtttcgat tgcgattcgc gttgtatcga agcgttttcg caacatcagt
     1141 aaaaacatgc agaacaccat ggggcaggtg accaccagcg cagaacaaat gctgaagggc
     1201 cacaaagaag tattgatttt cggtggtcag gaagtggaaa cgaaacgctt tgataaagtc
     1261 agcaaccgaa tgcgtcttca ggggatgaaa atggtttcag cctcttccat ctctgatccg
     1321 atcattcagc tgatcgcctc tttggcgctg gcgtttgttc tgtatgcggc gagcttccca
     1381 agtgtcatgg atagcctgac tgccggtacg attaccgttg ttttctcttc aatgattgca
     1441 ctgatgcgtc cgctgaaatc gctgaccaac gttaacgccc agttccagcg cggtatggcg
     1501 gcttgtcaga cgctgtttac cattctggac agtgagcagg agaaagatga aggtaagcgc
     1561 gtgatcgagc gtgcgactgg cgacgtggaa ttccgcaatg tcacctttac ttatccggga
     1621 cgtgacgtac ctgcattgcg taacatcaac ctgaaaattc cggcagggaa gacggttgct
     1681 ctggttggac gctctggttc gggtaaatca accatcgcca gcctgatcac gcgtttttac
     1741 gatattgatg aaggcgaaat cctgatggat ggtcacgatc tgcgcgagta taccctggcg
     1801 tcgttacgta accaggttgc tctggtgtcg cagaatgtcc atctgtttaa cgatacggtt
     1861 gctaacaaca ttgcttacgc acggactgaa cagtacagcc gtgagcaaat tgaagaagcg
     1921 gcgcgtatgg cctacgccat ggacttcatc aataagatgg ataacggtct cgatacagtg
     1981 attggtgaaa acggcgtgct gctctctggc ggtcagcgtc agcgtattgc tatcgctcga
     2041 gccttgttgc gtgatagccc gattctgatt ctggacgaag ctacctcggc tctggatacc
     2101 gaatccgaac gtgcgattca ggcggcactg gatgagttgc agaaaaaccg tacctctctg
     2161 gtgattgccc accgcttgtc taccattgaa aaggcagacg aaatcgtggt cgtcgaggat
     2221 ggtgtcattg tggaacgcgg tacgcataac gatttgcttg agcaccgcgg cgtttacgcg
     2281 caacttcaca aaatgcagtt tggccaatga tcgaaaaaat ctggtctggt gaatcccctt
     2341 tgtggcggct attgctgcca ctctcctggt tgtatggcct ggtgagtggc gcgatccgtc
     2401 tttgctataa actaaaactg aagcgcgcct ggcgtgcccc cgtaccggtt gtcgtggttg
     2461 gtaatctcac cgcaggcggc aacggaaaaa ccccggtcgt tgtctggctg gtggaacagt
     2521 tgcaacagcg cggtattcgc gtgggggtcg tatcgcgggg atatggtggt aaggctgaat
     2581 cttatccgct gttattgtcg gcagatacca caacagcaca ggcgggtgat gaacctgtgt
     2641 tgatttatca acgcactgat gcgcctgttg cggtttctcc cgttcgttct gatgcggtaa
     2701 aagccattct ggcgcaacac cctgatgtgc agatcatcgt aaccgacgac ggtttacagc
     2761 attaccgtct ggcgcgtaat gtggaaattg tcgttattga tggtgtgcgt cgctttggca
     2821 atggctggtg gttgccggcg gggccaatgc gtgagcgagc ggggcgctta aagtcggttg
     2881 atgcggtaat cgtcaacggc ggtgtccctc gcagcggtga aatccccatg catctgctgc
     2941 cgggtcaggc ggtgaattta cgtaccggta cgcgttgtga cgttgctcag cttgaacatg
     3001 tagtggcgat ggcggggatt gggcatccgc cgcgcttttt tgccacgctg aagatgtgtg
     3061 gcgtacaacc ggaaaaatgt gtaccgctgg ccgatcatca gtctttgaac catgcggatg
     3121 tcagtgcgtt ggtaagcgcc gggcaaacgc tggtaatgac tgaaaaagat gcggtgaaat
     3181 gccgggcctt tgcagaagaa aattggtggt atttgcctgt agacgcacag ctttcaggtg
     3241 atgaaccagc gaaactgctt acgcaactaa ccttgctggc ttctggcaac tagttacgcc
     3301 gcggcagcgt tcgattgatg gagtcatgaa tgtcgctgcc gcacctctcc cttgctgatg
     3361 cgcgtaatct tcaccttgcc gcacaaggcc tgttaaacaa accccgccgt cgagcgtcgt
     3421 tggaggatat tccggcaacg atctcccgca tgtccttgct gcaaatcg
//