From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: B.taurus mRNA for immunoglobulin G1 heavy chain
Date: 25 Jul 92 10:02:44 GMT
Distribution: bionet
Checksum: 62488     5

LOCUS       BOVIGG1HCX   1581 bp ss-RNA             MAM       25-JUL-1992
DEFINITION  B.taurus mRNA for immunoglobulin G1 heavy chain
ACCESSION   X62916
KEYWORDS    Ig heavy chain; IgG1; IgG1 heavy chain; anti-testosterone antibody.
SOURCE      Bos taurus RNA.
  ORGANISM  Bos taurus
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Artiodactyla; Ruminantia; Pecora; Bovidae; Bovinae;
            Bovini.
REFERENCE   1  (bases 1 to 1581)
  AUTHORS   Jackson,T.F., Morris,B.A. and Sanders,P.G.
  TITLE     Nucleotide sequences and expression of cDNAs for a bovine
            anti-testosterone monoclonal IgG1 antibody
  JOURNAL   Mol. Immunol. (1992) In press
  STANDARD  full automatic
COMMENT     *source: tissue=mouse x bovine heterohybridoma; *source: cell_line=
            B/MT.4A.17.H5.A5.; *source: is_germline=N;
            From EMBL    entry BTIGG1HCX;  dated 17-JUN-1992.
FEATURES             Location/Qualifiers
     CDS             22..1434
                     /product="anti-testosterone antibody"
                     /gene="immunoglobulin G1 heavy chain"
                     /codon_start=1
                     /translation="MNPLWTLLFVLSAPIGVLSQVQLRESGPSLVKPSQTLSLTCTVS
                     GFSLSSYALTWVRQAPGKALEWVGGITSGGTTYYNPALKSRLSITKENSKSQVSLSVS
                     SVTPEDTATYYCARSTYGEVGDGAIADAWGQGLLVTVSSASTTAPKVYPLSSCCGDKS
                     SSTVTLGCLVSSYMPEPVTVTWNSGALKSGVHTFPAVLQSSGLYSLSSMVTVPGSTSG
                     QTFTCNVAHPASSTKVDKAVDPTCKPSPCDCCPPPELPGGPSVFIFPPKPKDTLTISG
                     TPEVTCVVVDVGHDDPEVKFSWFVDDVEVNTATTKPREEQFNSTYRVVSALRIQHQDW
                     TGGKEFKCKVHNEGLPAPIVRTISRTKGPAREPQVYVLAPPQEELSKSTVSLTCMVTS
                     FYPDYIAVEWQRNGQPESEDKYGTTPPQLDADSSYFLYSKLRVDRNSWQEGDTYTCVV
                     MHEALHNHYTQKSTSKSAGK"
     sig_peptide     22..78
     mat_peptide     79..1431
                     /product="anti-testosterone antibody"
     misc_feature    79..444
                     /note="Vh domain"
     misc_feature    445..732
                     /note="Ch1 domain"
     misc_feature    733..771
                     /note="hinge"
     misc_feature    772..1101
                     /note="Ch2 domain"
     misc_feature    1102..1431
                     /note="Ch3 domain"
     polyA_signal    1546..1551
     polyA_site      1577
BASE COUNT      339 a    539 c    432 g    271 t
ORIGIN
        1 ctgactctca tctgctccaa gatgaaccca ctgtggaccc tcctctttgt gctgtcagcc
       61 cccatagggg tcctgtccca ggtgcagctg cgggagtcgg gccccagcct ggtgaagccc
      121 tcacagaccc tctccctcac gtgcacggtg tctggattct cattgagcag ctatgctcta
      181 acctgggtcc gccaggctcc agggaaggcg ctggagtggg ttggtggtat aaccagtggt
      241 ggaaccacat actataatcc agccctgaaa tcccggctca gcatcaccaa ggagaactcc
      301 aagagccaag tctctctgtc agtgagcagc gtgacacctg aggacacagc cacatactac
      361 tgtgcaagaa gtacttatgg tgaggttggt gatggtgcca tcgccgatgc ctggggccaa
      421 ggactcctgg tcaccgtctc ctcagcctcc accacagccc cgaaagtcta ccctctgagt
      481 tcttgctgcg gggacaagtc cagctccacc gtgaccctgg gctgcctggt ctccagctac
      541 atgcccgagc cggtgaccgt gacctggaac tcgggtgccc tgaagagcgg cgtgcacacc
      601 ttcccggctg tccttcagtc ctccgggctg tactctctca gcagcatggt gaccgtgccc
      661 ggcagcacct caggacagac cttcacctgc aacgtagccc acccggccag cagcaccaag
      721 gtggacaagg ctgttgatcc cacatgcaaa ccatcaccct gtgactgttg cccaccccct
      781 gagctccccg gaggaccctc tgtcttcatc ttcccaccga aacccaagga caccctcaca
      841 atctcgggaa cgcccgaggt cacgtgtgtg gtggtggacg tgggccacga tgaccccgag
      901 gtgaagttct cctggttcgt ggacgacgtg gaggtaaaca cagccacgac gaagccgaga
      961 gaggagcagt tcaacagcac ctaccgcgtg gtcagcgccc tgcgcatcca gcaccaggac
     1021 tggactggag gaaaggagtt caagtgcaag gtccacaacg aaggcctccc ggcccccatc
     1081 gtgaggacca tctccaggac caaagggccg gcccgggagc cgcaggtgta tgtcctggcc
     1141 ccaccccagg aagagctcag caaaagcacg gtcagcctca cctgcatggt caccagcttc
     1201 tacccagact acatcgccgt ggagtggcag agaaacgggc agcctgagtc ggaggacaag
     1261 tacggcacga ccccgcccca gctggacgcc gacagctcct acttcctgta cagcaagctc
     1321 agggtggaca ggaacagctg gcaggaagga gacacctaca cgtgtgtggt gatgcacgag
     1381 gccctgcaca atcactacac gcagaagtcc acctctaagt ctgcgggtaa atgagcctca
     1441 cgtccctgca ccagcaagcc ctcacccagc ccaccctccc cgggctccag gtccagccag
     1501 gacgccctag cccctccctg tgtgcattcc tcctgggccg ccgtgaataa agcacccagg
     1561 ccgccctggg accctgcaaa a
//