From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Acanthamoeba castellanii (clone pAr1) gene, complete ribosomal
Date: 16 Dec 92 23:23:03 GMT
Distribution: bionet
Checksum: 10614 7

LOCUS       ACAMTRGT     2675 bp ds-DNA             INV       15-DEC-1992
DEFINITION  Acanthamoeba castellanii (clone pAr1) gene, complete ribosomal
            intergenic spacer, partial 26S rRNA and 5'ETS flanking region.
ACCESSION   L07635
KEYWORDS    26S ribosomal RNA; enhancer; external transcribed spacer; promoter.
SOURCE      Acanthamoeba castellanii (strain Neff) trophozoite DNA.
  ORGANISM  Acanthamoeba castellanii
            Eukaryota; Animalia; Protozoa; Sarcomastigophora; Sarcodina;
            Rhizopoda; Lobosa; Gymnamoeba; Amoebida; Acanthopodina;
            Acanthamoebidae.
REFERENCE   1  (bases 1 to 2675)
  AUTHORS   Kownin,P., Iida,C.T., Brown-Shimer,S. and Paule,M.R.
  TITLE     The ribosomal RNA promoter of Acanthamoeba castellanii determined
            by transcription in a cell-free system
  JOURNAL   Nucleic Acids Res. 13, 6237-6248 (1985)
  STANDARD  full automatic
REFERENCE   2  (bases 1 to 2675)
  AUTHORS   Kownin,P., Bateman,E. and Paule,M.R.
  TITLE     Eukaryotic RNA Polymerase I Promoter Binding Is Directed by Protein
            Contacts with Transcription Initiation Factor and Is DNA
            Sequence-Independent
  JOURNAL   Cell 50, 693-699 (1987)
  STANDARD  full automatic
REFERENCE   3  (bases 1 to 2675)
  AUTHORS   Yang,Q., Zwick,M.G. and Paule,M.R.
  TITLE     Sequence organization of the Acanthamoeba rRNA intergenic spacer:
            Identification of transcriptional enhancers
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     rRNA            1..126
                     /product="26S ribosomal RNA"
                     /citation=[3]
                     /partial
                     /note="putative"
     misc_RNA        127..2456
                     /evidence="EXPERIMENTAL"
                     /citation=[3]
                     /partial
                     /note="rRNA intergenic spacer"
     enhancer        466..1296
                     /standard_name="rRNA enhancer"
                     /evidence="EXPERIMENTAL"
                     /citation=[3]
                     /note="six repeats (ave 139bp) enhance promoterin vitro in
                     cis, inhibit in trans"
     promoter        2410..2464
                     /evidence="EXPERIMENTAL"
                     /citation=[3]
                     /citation=[2]
                     /citation=[1]
                     /note="rRNA core promoter"
     misc_feature    2457..2675
                     /standard_name="external transcribed spacer"
                     /evidence="EXPERIMENTAL"
                     /label=ETS
                     /citation=[2]
                     /citation=[1]
                     /note="transcription start site is at 2457
                     (+1-AAAGGGAC.....)"
BASE COUNT      609 a    667 c    658 g    741 t
ORIGIN      
        1 gaattcgagc tcggtacccg gggatccttt gcagacgact tatataaaga acggggtatt
       61 gtaaggggca gagtagactt gtttctacga tcctctgaga ttaagccttt gttctcttga
      121 ttcgtatata tattatatta tattatatta tatatacacc cttctcctct ctttttcccc
      181 ctactcgctt atcgcttggg ggcttgtctc tatttgagtg agtgtgagtg agtgagagag
      241 acactctcgt ttctttgtca tgtgtgtttg tgtgttttgt gtgtttgtgt ttgtgtgtat
      301 ttgtgtttgt gtgttttgtg tttgtgcaca caaaacacac acgaatacac aaatacacaa
      361 atacacacac acacaaacac acaaaacaca caaacacaca aacaaacaca caaaacacac
      421 aaacaaacaa acacacaaac aaacaaacac aaacaaacaa acaaacacaa acacacacac
      481 acacacacac atctgctcgg gggcttggcc caacgacttg gcgtttttcg acgggtcgtg
      541 cggaaaatag gggtccgttg tgtgtttttg tgtttgtgtg tttgtgtgtg tttgtgtttg
      601 tgtgttttgt gttttgtgtt tgtgcacaaa cacacacaca caaacacatc tgcttggggg
      661 cttggcccaa cgacttggca tttttcgatg ggtcgtgcgg aaaatagggg tccgttgtgt
      721 ttgtttgtgt ttttgtgttt gtgtgtttgt gcacacacaa aacatgtttg cttgggggct
      781 tggcccaacg acttggcatt tttcggcggg tcgtgcggaa aataggggtc cgttgtgttt
      841 ttgtgtttgt gtgtttttgt gtttttgtgt ttttgtgttt gtgtgtgttt ttgtgtttgt
      901 gtgtgtttgt gtttgtgtgt ttgtgcacac aaaacatgtt tgcttggggg cttggcccaa
      961 cgacttggca tttttcgacg ggtcgtgcgg aaaaataggg gtccgttgtg tgtttttgtg
     1021 tttgtgtttt tgtgtttgtt tttgtgtttg tgtgtgtttg tgtttgtgtg tttgtgcaca
     1081 caaaacatgt ttgcttgggg gcttggccca acgacttggc atttttcgac gggtcgtgcg
     1141 gaaaatgggg gtccgttgtg tttttgtgtt tttgtgtttg tgcacacaca aaacatgttt
     1201 gcttgggggc ttggcccaac gacttggcat ttttcgacgg gtcgtgcgga aaaatagggg
     1261 tccgttgtgt gttttttgtg tttgtttgtg tttgtgcaca aacacaaaac acaaaacaca
     1321 aacacaaaca cacctagtcg gggacttgac ttggcatttt tcggccctgc tcgggggctt
     1381 gtaccgagcc gccaccccag tttttagacc ccactagggg gcttgttatg gccggccatg
     1441 tcgttttttg tgtggaatgg aatttgccaa gaccgccggt tcgaccacct ctttaggtgc
     1501 acccatgtgt cgcacatcaa aatgatgccc ctcgtgtgct ctatgcatga cccgatgcga
     1561 tgggcgcgtt cgtgcgggcg tgttcgatct ggacgctcgc ctcgccaaag ttcggtcccc
     1621 cccggccaat tttgaccccc ttttttcgta ttttaattct cgcgccccaa cggctgcgat
     1681 tttccacctt cccattgcta tccgatgctc cccgcgctct ggcctatcca acgaacccag
     1741 cctcgcccgt gcacctatcc gtttgccctc gttgaccctc ccaaacgcct gacatcacct
     1801 atgccattta tgcaacaaat tcgacctcac gcccgtgcaa tcacaagtac gcacaaacaa
     1861 acgtatatgc acgcaaccac acccccaggc acacacacaa acgtgcacac cgacacgaca
     1921 cgcaaacaca cggatgcaca aacgcgaacg caccgaaaaa cacgcagacg cacaaacgca
     1981 acacacgcaa cacacacgcc cacaaacatg cgcacggaca cgaacgacac aacaggggga
     2041 cacaacaaat gccaacgcac acgcacaagc atgcacaaac acgacatgca ctcaaacaca
     2101 ccggcacaac atttaaaatt ggtctcaaac accacacagg caccacacac aaacacgcac
     2161 agacacgccc atgcggggtg ggaggggggg cttgtccctc tggctcgatg cacgtggtca
     2221 cgcttgtggg tttgtgtgtg tgtgtgtgtg tgcttttgcc ttcacgttgt tgtgccacac
     2281 acacacaaac acgacctcat gaaaaaaaac acgtttgctt gggggcttgc tctagggact
     2341 ttgctgctgc aaggtgtctc ggccgggccg gtggccggaa aaatcccggc aaaccgcaaa
     2401 ccggccggag cacttttctg gcacctaaac tggtcggacc gtccgaaagt atatataaag
     2461 ggacgggtcc ggccggaaaa aaaaaatacg cccattaagg cagtccgctg ccgttttgga
     2521 ccaccgtatt ttagacctcg gttgggcatt aaataggtcc ccccccacag gcactccccc
     2581 cgccctatgt cgcctactct tgagtagagt agggggcaca gacaggggcg gcagtgcggc
     2641 ctagtgttga tccagtcccg tctcgttgtg tctga
//