From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: D.pseudoobscura Adh gene for alcohol dehydrogenase
Date: 28 Dec 92 21:27:50 GMT
Distribution: bionet
Checksum: 08225 8

LOCUS       DROADHGN66   3470 bp ds-DNA             INV       01-DEC-1992
DEFINITION  D.pseudoobscura Adh gene for alcohol dehydrogenase
ACCESSION   X64475
KEYWORDS    ADH gene; alcohol dehydrogenase.
SOURCE      Drosophila pseudoobscura DNA.
  ORGANISM  Drosophila pseudoobscura
            Eukaryota; Animalia; Metazoa; Arthropoda; Uniramia; Insecta;
            Pterygota; Neoptera; Holometabola; Diptera; Brachycera;
            Cyclorrhapha; Schizophora; Drosophiloidea; Drosophilidae.
REFERENCE   1  (bases 1 to 3470)
  AUTHORS   Schaeffer,S.W. and Miller,E.L.
  TITLE     Estimates of gene flow in Drosophila pseudoobscura determined from
            nucleotide sequence analysis of the alcohol dehydrogenase region
  JOURNAL   Genetics 132, 471-480 (1992)
  STANDARD  full automatic
COMMENT     *source: strain=PS224; *source: isolate=Kiabab National Forest,
            Arizona; *source: chromosome=IV section 88;
               From EMBL    entry DPADHGN66;  dated 16-OCT-1992.
FEATURES             Location/Qualifiers
     mRNA            join(40..119,915..1020,1084..1488,1549..1964)
                     /note="adult mRNA (Adh)"
     mRNA            join(882..1020,1084..1488,1549..1964)
                     /note="larval mRNA (Adh)"
     promoter        8..14
                     /note="adult promoter (Adh)"
     prim_transcript 40..1964
                     /note="adult primary transcript (Adh)"
     exon            40..119
                     /note="adult exon 1a"
     intron          120..914
                     /note="adult intron"
     prim_transcript 882..1964
                     /note="larval primary transcript (Adh)"
     exon            882..1020
                     /note="larval exon 1"
     exon            915..1020
                     /note="adult exon 1b"
     intron          1021..1083
                     /number=1
     exon            1084..1488
                     /number=2
     intron          1489..1548
                     /number=2
     exon            1549..1964
                     /number=3
     polyA_signal    1941..1946
     polyA_site      1964
     CDS             join(928..1020,1084..1488,1549..1815)
                     /EC_number="1.1.1.1"
                     /gene="Adh"
                     /product="alcoholdehydrogenase"
                     /codon_start=1
                     /translation="MSLTNKNVVFVAGLGGIGLDTSRELVKRNLKNLVILDRIDNPAA
                     IAELKAINPKVTITFYPYDVTVPVAETTKLLKTIFAQVKTIDVLINGAGILDDHQIER
                     TIAVNYTGLVNTTTAILDFWDKRKGGPGGIICNIGSVTGFNAIYQVPVYSGSKAAVVN
                     FTSSLAKLAPITGVTAYTVNPGITKTTLVHKFNSWLDVEPRVAEKLLEHPTQTSQQCA
                     ENFVKAIELNKNGAIWKLDLGTLEPITWTQHWDSGI"
BASE COUNT      967 a    793 c    798 g    912 t
ORIGIN      
        1 cgtagtgtat ttaaggcact gcacatgtcg aggaccacga ttattgtcta cagtgcagtc
       61 agtagttgtc aggtgcagca ggcggaccag catttcttta aatctactca atcgatcaag
      121 taagtaactg taatccaagt acagtcggcc catcccactc ggttctgccg taactcaaga
      181 aatttcttga tagcgcagtt ccagttgtag aacgtcgttt aaaaaaacat ctcgttggag
      241 cttgggttgg aaaacgttcg agttgagcgc agaaaatttc aaatataaaa tatcaaaaaa
      301 gagaaaatat cttcccaaag cagctgtcca ttttttgtta aagtgataga tcaaaaattc
      361 gtatgacgta tttcccaagt gctctttgtg caaataattt cctcaattct aattctacaa
      421 gaagcacttt tggctcttgt ccagaaaaaa caatttgttt acacttgtcg gttgataaca
      481 gaggcggacc gacggctaag taacataaca gtcgcctgtt aagtaatgcc ctcactctct
      541 gttagatggc cgcgcgtgtc gactccgata gaccagcaga cacgcagtag ttttcctcaa
      601 gaacctgaac tctaaacata gacataattt acgatacctt gagactcaaa taaaaataca
      661 tgtatgagtg gtattctatt cctgattaga ctcgcacaca cacacgtacg agtattaaca
      721 taaatttgcc ggcaaataac caaaacaaaa cgaacgcaaa cgatccgaac acgtaatgcg
      781 agagataaga aacagaaaag ctcgacgtga aagaagcttc tggcggaagc tcttgccgaa
      841 ctccctataa atacgagaca gaaaaccaga ataaatataa taacaaactg ttgaattgtc
      901 ccatcgattc ccagatcaaa agacagaatg tctctcacca acaagaacgt cgttttcgtg
      961 gccggtctgg gcggcattgg cctggacacc agccgggagt tggtcaagcg taatctgaag
     1021 gtaagagaga acgaattcca tggaatctat ggaatcctaa atttaaaaat acattttttt
     1081 tagaacctgg tcatcctgga tcgcattgac aatccggctg ccattgccga actgaaggca
     1141 atcaatccca aggtgaccat caccttctat ccctacgatg tgactgtgcc cgtcgctgag
     1201 accaccaagc tcctgaagac catctttgcc caggtgaaga ccatcgatgt cctgatcaac
     1261 ggtgctggca tcctggacga tcatcagatc gagcgcacca ttgccgttaa ctacacgggc
     1321 ctggtcaaca ccaccacagc cattctggac ttctgggaca agcgcaaggg cggcccaggc
     1381 ggcatcattt gcaacattgg ctccgtcacc ggtttcaatg ccatctacca ggtgcccgtt
     1441 tactccggct ccaaggcggc ggtggttaac tttacctcct ccctggcggt aagtaatcca
     1501 acctttttcc tactacccaa aactaaatat ttctttgaaa tcccatagaa actggctccc
     1561 attactggtg tcactgctta cactgtcaat cctggcatca ctaagaccac tctggtccac
     1621 aaattcaact cgtggctgga tgtggagccc cgtgtggcgg agaagctgct cgagcatccc
     1681 acccagacct ctcagcagtg cgccgagaac tttgtgaagg ccattgagct gaacaagaac
     1741 ggtgccatct ggaagttgga tttgggcacc ttggagccca tcacatggac ccagcactgg
     1801 gactcgggca tctaaaccat cccagagact ctatgggaca tggcgtttta gcttttagtt
     1861 tctttttttc cactcaattg ttacgtatat acatacacat atggcaataa ggctgatttg
     1921 aaccccgttt tgaatatgat aatacaaaat tatatttgag aaatttcaac aaaatcgata
     1981 agagctatag ctctctttta attaaaaaaa actagataaa aagagcaatg gtcagtggtg
     2041 gtgggcagtg gtgtggtgtg gcctctatcg atttccacac aaaaacttta ctgtttagta
     2101 atagaaaaga acttgagaaa ggcagccaaa agaatgtacg atctgacggg taagcatgtc
     2161 tgctatgtgg ctgactgcgg tggcattgca ctggagacca gcaaggttct catgaccaag
     2221 aatatagcgg tgagtggtag agtggtgtag agagtggaga gtggttctgg ttcttaaaca
     2281 agtctctagt ctctagtctc ttggctgctg ctggtagctg tcgaaactaa ttaagttaat
     2341 gactttttca tgttttgttt ttgtccgctg acttttgctg ttcaaagcta caattaagtt
     2401 tatgttttga gtttgatcta cttttgctta agggaagtac tttcgattac ggattgctgg
     2461 catataaaaa tagataccaa ggaatgtgca tcatatcata ttgattctat cttgattctt
     2521 tgcccagaaa ctggccatcc ttcagagcgt ggaaaacccc ccggccatcg cccagttaca
     2581 atcgattaag cacagcacac agatcttctt ctggaccttc gatgtgacca tggcccgcga
     2641 ggagatgaag aagtacttcg atgaggtcat ggtccagatg gactacatcg atgtgctgat
     2701 caatggggcc accctgtgcg atgagcggaa cattgatgcc accatcaata cgaatctgac
     2761 cggcatgatg aacaccgtgg ccactgtgct gccctatatg gaccgtaaga tgggcggttc
     2821 gggtggactg atcgtgaatg tcacctccgt cataggactg gatccatcgc cagtcttttg
     2881 tgcgtacagt gcctcaaagt ttggtgtgat tgggttcacc cgaagtctag cggtgagtgg
     2941 gcgagatctt aacttgaaaa tgctctctaa tgtcttccat tctttcgcag gatcccctgt
     3001 attacaccca aaatggtgtg gctgtcatgg ccgtctgctg tggtcccacc aaagtgtttg
     3061 tcgatcggga actgactgcc ttcctggcat acggccaaac ctttgccgat cgcctgcgtc
     3121 gtgccccctg ccaatcgacg gccgtctgcg gccagaatat agtaaatgcc atcgagagat
     3181 cggagaacgg acagatctgg attgccgaca agggcggcct ggagtcggtg gcccttcact
     3241 ggtattggca tatggccgat cagtttgtga actatatgca gagcactgac gatgaagatc
     3301 aggagttttt tttgggccag cgatgaggca tgccttgatc cacggaataa aacatcaaat
     3361 gtgtcacaaa aagtaggcaa cttgtttgtg ggtctggaaa tagatttgaa atattttgag
     3421 gtcatatgaa taatgacata aatgagggtg cctgaaagta tgccaagttt
//