From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Penicillium roqueforti aristolochene synthase (ariL) gene, complete
Date: 6 Nov 92 18:33:52 GMT
Distribution: bionet
Checksum: 51544 5

LOCUS       PECARIL      1562 bp ds-DNA             PLN       02-NOV-1992
DEFINITION  Penicillium roqueforti aristolochene synthase (ariL) gene, complete
            cds.
ACCESSION   L05193
KEYWORDS    Ari1 gene; aristolochene synthase; sesquiterpene cyclase.
SOURCE      Penicillium roqueforti (library: NRRL 849) DNA.
  ORGANISM  Penicillium roqueforti
            Eukaryota; Fungi; Eumycota; Ascomycotina; Plectomycetes;
            Eurotiales; Trichocomaceae.
REFERENCE   1  (bases 1 to 1562)
  AUTHORS   Proctor,R.H. and Hohn,T.M.
  TITLE     Aristolochene synthase. Isolation, characterization, and bacterial
            expression of a sesquiterpenoid biosynthetic gene (Ari1) from
            Penicillium roqueforti
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     exon            <220..565
                     /number=1
                     /gene="Ari1"
     exon            <220..565
                     /number=1
                     /gene="Ari1"
     intron          566..614
                     /number=1
                     /gene="Ari1"
     exon            615..888
                     /gene="Ari1"
     intron          889..939
                     /gene="Ari1"
     exon            940..>1348
                     /number=3
                     /gene="Ari1"
     exon            940..>1348
                     /number=3
                     /gene="Ari1"
     CDS             join(220..565,615..888,940..1348)
                     /function="cyclization of farnesyl pyrophosphate to
                     aristolochene"
                     /standard_name="sesquiterpene cyclase"
                     /note="putative"
                     /gene="Ari1"
                     /product="aristolochene synthase"
                     /codon_start=1
                     /translation="MATSTETISSLAQPFVHLENPINSPLVKETIRPRNDTTITPPPT
                     QWSYLCHPRVKEVQDEVDGYFLENWKFPSFKAVRTFLDAKFSEVTCLYFPLALDDRIH
                     FACRLLTVLFLIDDVLEHMSFADGEAYNNRLIPISRGDVLPDRTKPEEFILYDLWESM
                     RAHDAELANEVLEPTFVFMRAQTDRARLSIHELGHYLEYREKDVGKALLSALMRFSMG
                     LRLSADELQDMKALEANCAKQLSVVNDIYSYDKEEEASRTGHKEGAFLCSAVKVLAEE
                     SKLGIPATKRVLWSMTREWETVHDEIVAEKIASPDGCSEAAKAYMKGLEYQMSGNEQW
                     SKTTRRYN"
BASE COUNT      397 a    386 c    398 g    381 t
ORIGIN      
        1 tggttattga ccgggcgcga actcggaaag aacaccagat gtgacagttc gaattagttt
       61 catccaggtc gtcggagtcc tacgtacacg atcaattcga gctcctgata agactggacg
      121 agtttccgaa gtctggtatt ttttcgacga tatgcttcct cgcacttact gaaagctgtt
      181 atccacgagt ataacccttc aaacccggtg agcgtcgaca tggctacctc aacagaaacc
      241 atttcttccc tggcccaacc gttcgtgcac cttgaaaacc ctatcaatag ccctctggtc
      301 aaagagacaa tcaggcccag aaatgacacg acgatcactc cgcctcctac tcagtggtcg
      361 tacctttgcc atccacgagt gaaggaggta caggacgaag tcgatggata cttcctggag
      421 aactggaaat ttcccagctt caaggctgtt cgcaccttct tggatgccaa gttctcagag
      481 gttacttgtc tttacttccc tcttgcactg gacgatcgca tccactttgc ctgccgactg
      541 ctgaccgttc tcttccttat cgatggtgag tgcgaaagga catggcctaa tgtagccttc
      601 actgacgagg gtagatgttc ttgagcatat gtctttcgcg gatggagaag cctacaacaa
      661 cagattgatc ccgatatcgc gtggagacgt gctcccggac cgaaccaagc cagaagagtt
      721 cattctctat gacctctggg aaagcatgcg cgcccatgat gcggagctgg ccaacgaagt
      781 tcttgagccg actttcgtgt tcatgcgcgc gcagacagat cgagcgcgtt tgagcatcca
      841 tgaattgggg cattatctcg agtaccgtga gaaggatgta ggcaaggcgt atgtgttctc
      901 gcccaggatt cgagctcgtg atactgactg ttcggccagg ttgctttcgg ctctaatgag
      961 attctcgatg ggacttagac tcagtgcaga tgagcttcag gatatgaaag cccttgaagc
     1021 caactgtgcc aagcagcttt ctgtagtcaa tgacatatac agttatgaca aggaagagga
     1081 agcgtctcgg actggacaca aggagggagc cttcctttgt tcagctgtga aggttctggc
     1141 ggaggagtcc aagcttggta tacccgcaac gaaacgcgtg ctttggtcta tgactcggga
     1201 gtgggagact gtgcatgacg agatcgtggc agagaagatc gcatccccag acggctgctc
     1261 tgaagctgcc aaggcgtata tgaagggcct agagtaccag atgagcggca atgagcagtg
     1321 gagcaagacc acgcgtcgct acaactaaat gtatagattc agcaagaata gatataagca
     1381 tgaagcgcgt tacacagatt aagcttaagc aagatgcatt gtgcgaccct caaaccccct
     1441 ttttagggga atcctgatgc cttgatacct gaattagtac tgctttctat accgccctaa
     1501 tacagtgtac ggtgaattgt caatacatca acctgcctag atgaagcttt tggacaccat
     1561 ac
//