From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Synechococcus sp. ferredoxin NADP oxidoreductase (petH) gene, 5'
Date: 21 Aug 92 10:12:56 GMT
Distribution: bionet
Checksum: 51002     9

LOCUS       SYOFNND      4592 bp ds-DNA             BCT       21-AUG-1992
DEFINITION  Synechococcus sp. ferredoxin NADP oxidoreductase (petH) gene, 5'
            end, NADH dehydrogenase subunit 5 (ndhF) gene, complete cds.
ACCESSION   M99378
KEYWORDS    NADH dehydrogenase subunit 5; ferredoxin-NADP oxidoreductase;
            ndhF gene; petH gene.
SOURCE      Synechococcus sp. (individual_isolate PR6000, strain PR6) (library:
            PCC 7002) DNA.
  ORGANISM  Synechococcus sp.
            Prokaryotae; Gracilicutes; Oxyphotobacteria; Cyanobacteria;
            Chroococcales.
REFERENCE   1  (bases 1 to 4592)
  AUTHORS   Schluchter,W.M., Zhao,J. and Bryant,D.A.
  TITLE     Isolation and characterization of the ndhF gene of Synechococcus
            sp. PCC 7002 and initial characterization of an interposon mutant
  JOURNAL   Unpublished (1992)
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     CDS             complement(1..>54)
                     /EC_number="1.18.1.2"
                     /product="ferredoxin-NADP oxidoreductase"
                     /gene="petH"
                     /codon_start=1
                     /translation="MYGITSTANSTGNQSYAN"
     RBS             complement(64..69)
                     /gene="petH"
     RBS             713..717
                     /gene="ndhF"
     RBS             722..724
                     /gene="ndhF"
     CDS             733..2727
                     /product="NADH dehydrogenase subunit 5"
                     /gene="ndhF"
                     /codon_start=1
                     /translation="MEPLYQYAWLIPVLPLLGAMVIGIGLISLNKFTNKLRQLYAVFV
                     LSLIGTSMALSFGLLWSQIQGHEAFTYTLEWAAAGDFHLQMGYTVDHLSALMSVIVTT
                     VALLVMIYTDGYMAHDPGYVRFYAYLSIFSSSMLGLVFSPNLVQVYIFWELVGMCSYL
                     LIGFWYDRKAAADACQKAFVTNRVGDFGLLLGMLGLYWATGSFEFDLMGDRLMDLVST
                     GQISSLLAIVFAVLVFLGPVAKSAQFPLHVWLPDAMEGPTPISALIHAATMVAAGVFL
                     VARMYPVFEPIPEAMNVIAWTGATTAFLGATIALTQNDIKKGLAYSTMSQLGYMVMAM
                     GIGGYTAGLFHLMTHAYFKAMLFLGSGSVIHGMEEVVGHNAVLAQDMRLMGGLRKYMP
                     ITATTFLIGTLAICGIPPFAGFWSKDEILGLAFEANPVLWFIGWATAGMTAFYMFRMY
                     FLTFEGEFRGTDQQLQEKLLTAAGQAPEEGHHGSKPHESPLTMTFPLMALAVPSVLIG
                     LLGVPWGNRFEAFVFSPNEAAEAAEHGFELTEFLIMGGNSVGIALIGITIASLMYLQQ
                     RIDPARLAEKFPVLYQLSLNKWYFDDIYNNVFVMGTRRLARQILEVDYRVVDGAVNLT
                     GIATLLSGEGLKYIENGRVQFYALIVFGAVLGFVIFFSVA"
     stem_loop       2747..2799
                     /gene="ndhF"
BASE COUNT      972 a   1070 c   1254 g   1296 t
ORIGIN
        1 attggcgtag gattgatttc cagtagagtt tgctgtgcta gtgataccgt acattatgtg
       61 cagtttccca attattttga tttatcagtt tttttgatct taaagggggt ggatcgtcgg
      121 tacaaaccgc tttttcccgc tttagagtca agattataag ggctttactc catttagact
      181 atcattgtgc tgcccatgct ttactgaatg gggcgggaag tgcgactgat tttgatgtat
      241 cagaattggg atgcaaggcc actggaaaca cgatcttagc cgggctcggt ccttggagtt
      301 agttagtgca agattgtgag tcaaggaagt ttagaaagac attttaggga tgtttctcta
      361 ggggttttgt gatggctgtt gcattcttct tggggctttg taaggggatc ttcatagagg
      421 taatcttttt agattttctt tagatttttt tgaaatggat tagaacgggg cgatcgcctt
      481 tgctggggaa ttgtgatagc aaaatataat caaacggcgg taatccgttg aagatttagg
      541 gggatatgtc acaatgaggg gcgaaactct ttgaccattg gtgtctattt agaacagccc
      601 aaacagagca actcgcccca agcaattccg aaaaatgccc ttggtcaact gtgcgctgtc
      661 acccaaaatt tgttgggaaa tttgacatca atggatgtgt aacttagaaa gcaagaatcc
      721 taggcattcg taatggaacc gctttatcaa tatgcatggt tgattccggt gctgcctctg
      781 ttgggggcaa tggttatcgg gatcggctta atctccttaa acaaattcac gaacaagcta
      841 cggcaactct atgcggtatt tgttctgtcg ctcatcggca cttcgatggc cctctctttt
      901 gggctactct ggagtcaaat tcagggtcat gaagccttta cctacaccct agaatgggcg
      961 gcggcaggcg attttcatct gcagatgggc tataccgtgg atcacctgag tgccttgatg
     1021 tcggtgatcg taaccacggt ggccttgctg gtgatgatct ataccgatgg ttacatggcc
     1081 cacgatccgg gttatgtccg tttttatgct tatttgagca tttttagctc gtcgatgttg
     1141 ggcttggtgt ttagcccaaa ccttgtgcag gtttacattt tctgggaatt ggtggggatg
     1201 tgttcctacc tcctgattgg cttctggtat gaccgcaagg cggcggccga tgcttgccaa
     1261 aaggcttttg tcacaaaccg tgtcggcgat tttggtctct tgctggggat gctcggtctc
     1321 tattgggcca cgggcagttt tgagtttgat ctgatgggcg atcgcctgat ggatctcgtc
     1381 tcgacggggc aaatcagctc tctcctggcc attgtctttg ctgtacttgt gttccttggc
     1441 cctgtggcta aatccgccca atttccgctc catgtgtggc tccccgatgc catggaaggg
     1501 ccaaccccca tttccgcttt gatccacgcg gcgaccatgg ttgctgctgg agtgttcctc
     1561 gtggcgcgga tgtatcccgt gtttgagccg atcccagagg cgatgaatgt cattgcctgg
     1621 acgggggcaa ccaccgcttt tctaggggcg accattgcct taacccaaaa cgacatcaaa
     1681 aaaggtcttg cctattccac catgtcccag ttgggctaca tggtgatggc catggggatc
     1741 ggtggctaca ccgctgggtt gtttcacctg atgacccatg cctacttcaa agcgatgctc
     1801 ttcttgggtt ccggttctgt catccatggt atggaagagg tggtcggcca taatgctgtg
     1861 ctggcccagg atatgcggct gatgggtggc ctgcgaaaat atatgcccat cactgccaca
     1921 acgtttctca ttgggaccct cgctatctgt gggatcccgc cctttgctgg tttctggtca
     1981 aaggacgaaa ttttgggcct ggcgtttgag gccaatcctg tgctctggtt cattggttgg
     2041 gccaccgctg gcatgaccgc tttttatatg ttccggatgt acttcctgac ctttgaaggg
     2101 gagtttcgcg gcacggatca gcaactgcaa gaaaagctcc tgacggcggc aggtcaagcc
     2161 cccgaagaag gccaccatgg ctctaagccc cacgagtctc ctttgacgat gactttcccg
     2221 ttgatggctt tggcagttcc gtcggtattg atcggtttgc tgggggttcc ctggggtaat
     2281 cggtttgaag cttttgtctt ttctcccaac gaagcagcag aggccgctga gcatggtttt
     2341 gaattgacag aatttttgat catgggtggt aattctgtgg ggatcgccct cattgggatt
     2401 accattgcct cgctgatgta tctccagcag agaattgatc cggctcgttt ggccgaaaaa
     2461 ttcccggtgt tgtatcagtt gtctttgaac aaatggtact tcgacgatat ctacaacaat
     2521 gtttttgtga tgggtacccg gcgcttagcc cgacaaatcc ttgaggtgga ctatcgcgtc
     2581 gtcgatgggg ccgtaaacct cacggggatc gccactctcc tgagtggtga gggcttgaaa
     2641 tacattgaaa atggccgcgt ccagttctat gcgctcattg tgtttggggc ggtgctaggc
     2701 ttcgtcattt tctttagtgt ggcctagccg tcactatatt ccgaaagttg agaagataaa
     2761 atttcccctt gcctgagtgg tgaggggttt tatttttcct gataagattt gcagtggcgt
     2821 tgttacaggg gttagcagaa aaattatggc tgaatttaaa caaggaaccc aggtaaagct
     2881 gacacagttg cctccctatc tcaagaccgc agacccgatg ccgatgttgc gtccggcgga
     2941 tttattggcg gtgggggcgg ttggcaccct aggcgatcgc cgccctggtg gttattgggc
     3001 ggttaagttc gagcggggct cttttttgct tgaagaaacc tatctagcag tcgccactgg
     3061 gacggcctaa acctaggaat tgggcagatc gacagccgga ctcaggggga caaaatcgcc
     3121 gtgaatagtg aggccgaggc acatggtttc gcctgtttgg atgcgtttcc ccgtaagggg
     3181 cgcaggtttc atcctttttg gcgatcgcct caagggtcgc ccgaatatct gcttcgctaa
     3241 actgggggcg ataatcccct gagttgtgat tgacgtagtt ccagagaata tcgcggatta
     3301 gagcttggta tccctggttt ccagcgagtt ccttgagttt atctttcagt ttgcgctcta
     3361 ggcgaatact ggtgacttcc atttctgtcg tgctagttgc ccgagtcata attttcgctg
     3421 atccccttac atttaagact ttactaaata tctgtcctcg gaatgaggca aaacatcaaa
     3481 agttgtagtg tcagtgtagt gaaaatgttt tagaaaaaaa agcgcgattt ggctgaattg
     3541 ttactcattg acaatatcta aggcgatcgc ctccgccact ttgaccccat caatggctgc
     3601 cgagagaatg ccccctgcat aaccagctcc ctcccccgca ggatagaggc cctgggtatt
     3661 aatactttgg taatctttgc cccgtttaat gcgaattggc gaagaggtgc gcgtttccac
     3721 ccccgttaac atcgcctcag ccatggcaaa ccccttaatt tgtttgtcaa aagcgggtag
     3781 agcttcccga atcgcttcga tggcgtagtc cgggagcgcg gtgctgagat ccgttagctt
     3841 aactcccggg gcataggaag gttttacttt gcccaacgac tcagaagaac aacccgcgag
     3901 gaagtccccc accagttgac ccggcgcgct gtaatcacca ccacccagtt caaaggccct
     3961 ggcttctaat tttcgttgga gatcaatgcc tgcgagggga tgacctggaa aatcctcttc
     4021 tggggtaatg ccgacaacga tcccgcattg gcgttacgtt cgttgcggga atattggctc
     4081 atgccgttgg tgacgacttt gcctgcctcg gaggtggccc caatcaccaa tccccccgga
     4141 cacatacaaa agctatagac cgagcgccca ttttgacagt ggtggacgag cttgtaatcg
     4201 gcggcaccga gaatcttatt gccagcaaat tccccgtagc gacattcatc gatcaggggt
     4261 tggggatgtt caatgcgaaa gccaatggaa aagggcttgg gttcgatgta aacgccctgg
     4321 tcatggagca tttggaaggt atcccgcgca ctgtggccca tggccagcac cacgtgggaa
     4381 ctgggaataa attcgccatt ttcgagggtg acgcctttga cctgatggtt ttctagttcg
     4441 agggttttga ccttgctttg gaaacgaatt tcgccgccga gggattcgat ggttttgcga
     4501 atgctttgga cgatgcccac gagtttaaag gtgccgatgt ggggcttggc gatgtagagg
     4561 atttcgggat tggccccggc ggcgacgaat tc
//