From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: P.americana cel1 gene for cellulase (endo-beta-1,4-glucanase)
Date: 28 Jul 92 10:02:33 GMT
Distribution: bionet
Checksum: 47786    10

LOCUS       AVOCELLG     5081 bp ds-DNA             PLN       28-JUL-1992
DEFINITION  P.americana cel1 gene for cellulase (endo-beta-1,4-glucanase)
ACCESSION   X59944 X55789
KEYWORDS    cel1 gene; cellulase; endo-beta-1,4-glucanase.
SOURCE      Persea americana DNA.
  ORGANISM  Persea americana
            Eukaryota; Plantae; Embryobionta; Magnoliophyta; Magnoliopsida;
            Magnoliidae; Laurales; Lauraceae.
REFERENCE   1  (bases 8 to 5081)
  AUTHORS   Cass,L.G., Kirven,K.A. and Christoffersen,R.E.
  TITLE     Isolation and characterization of a cellulase gene family member
            expressed during avocado fruit ripening
  JOURNAL   Mol. Gen. Genet. 223, 76-86 (1990)
  STANDARD  full automatic
REFERENCE   2  (bases 1 to 5081)
  AUTHORS   Cass,L.G.
  JOURNAL   Unpublished (1991)
  STANDARD  full automatic
COMMENT     *source: strain=cv. Haas; *source: stage=ripe fruit; *source:
            clone_library=EMBL3; *source: clone=CEL1-6;
            See M17634 for overlapping sequence
            From EMBL    entry PACELLG;  dated 01-JUL-1992.
FEATURES             Location/Qualifiers
     CDS             join(1624..1833,1922..2131,2923..3123,3210..3302,
                     3408..3569,3650..3761,4159..4322,4409..4741)
                     /EC_number="3.2.1.4"
                     /product="cellulase"
                     /gene="cel1"
                     /codon_start=1
                     /translation="MDCSSPLSLFHLLLVCTVMVKCCSASDLHYSDALEKSILFFEGQ
                     RSGKLPTNQRLTWRGDSGLSDGSSYHVDLVGGYYDAGDNLKFGLPMAFTTTMLAWGII
                     EFGCLMPEQVENARAALRWSTDYLLKASTATSNSLYVQVGEPNADHRCWERPEDMDTP
                     RNVYKVSTQNPGSDVAAETAAALAAASIVFGDSDSSYSTKLLHTAVKVFEFADQYRGS
                     YSDSLGSVVCPFYCSYSGYNDELLWGASWLHRASQNASYMTYIQSNGHTLGADDDDYS
                     FSWDDKRVGTKVLLSKGFLQDRIEELQLYKVHTDNYICSLIPGTSSFQAQYTPGGLLY
                     KGSASNLQYVTSTAFLLLTYANYLNSSGGHASCGTTTVTAKNLISLAKKQVDYILGQN
                     PAKMSYMVGFGERYPQHVHHRGSSLPSVQVHPNSIPCNAGFQYLYSSPPNPNILVGAI
                     LGGPDNRDSFSDDRNNYQQSEPATYINAPLVGALAFFAANPVTE"
     mRNA            join(1508..1833,1922..2131,2923..3123,3210..3302,
                     3408..3569,3650..3761,4159..4322,4409..5005)
                     /gene="cel1"
                     /evidence=EXPERIMENTAL
     misc_feature    1153..1172
                     /note="GAGA box"
     CAAT_signal     1438..1450
     TATA_signal     1475..1480
     exon            1508..1833
                     /number=1
     intron          1834..1921
                     /number=1
     exon            1922..2131
                     /number=2
     intron          2132..2922
                     /number=2
     exon            2923..3123
                     /number=3
     intron          3124..3209
                     /number=3
     exon            3210..3302
                     /number=4
     intron          3303..3407
                     /number=4
     exon            3408..3569
                     /number=5
     intron          3570..3649
                     /number=5
     exon            3650..3761
                     /number=6
     intron          3762..4158
                     /number=6
     exon            4159..4322
                     /number=7
     intron          4323..4408
                     /number=7
     exon            4409..5005
                     /number=8
BASE COUNT     1396 a   1083 c   1029 g   1573 t
ORIGIN
        1 gtcgacgctt ttaattcagg tggctctttc ttttgattag cccacctgag tcttttaacg
       61 gctggatggg aatgtccgca tgcagatatt acctagggag aaaacttaag ctccaaacaa
      121 gttacaacaa tcaggcttaa gtgaaagttt aacaagaaaa taaagggtaa gactctgaaa
      181 gagtgttttt agtgtgagtg ttagggtaat gagttttctc ccttttgttc tcactccaac
      241 gtccagtttt ctgcagctga tcccgtgaac agcaccggcg gaatcatagg agggccggca
      301 ggggccattg gcccctccac tgccacccaa aatttcaaaa aacccaccct ttgtcttttc
      361 aaaatttata atctttttac attatggtcc ccccaaagtt ttcataaaag cctattttga
      421 aaactgcatt cattttttat gtggcccttt ccaaaaaaat ttctacatag tggccccctc
      481 aaggaagaat tcatgggtct gcccctgcaa ataggtgaga tatgggggga attagtgggt
      541 caaggttaga ttttgctgca tctaattgcg aaaggttaga gatataaggt ggaaatttgg
      601 atgagacctt gactgtctta taatttaatt tattaaggtg atggctgagt attcatttta
      661 taacttcctt tagttcattt tttaaccaaa cggaacatta ttcccataat tttaaacaac
      721 gcccccccgc gccccccaac ttttaaagaa aattctatga tttttcacat acagcccctc
      781 caaaatttct aaaaaacccc tgtttcttta gaaaattatt tcttttacat atggacccct
      841 gacatttcaa aaaatctact atatattttg aaaattacat acatttctta tatgtgggct
      901 cccaagaact tcttttctac ccagtgcccc cggcctccca ataatttatt ttctactgtt
      961 ttctcaaaaa aatggacttc tcttaagagt tttgcacgta tgcttcaccc ttctttctta
     1021 agctagagaa ttgctaactt tatgcagtta gttgaaattt cttccatcgc tggttggttt
     1081 taaaaaaggg ttgcaggcat gcagcttgaa ttttaaaaaa gggttgcagg tatggcgctc
     1141 gaacgtaaat atgagagaga gagagagaga gattgagatt ttgaagtcct aaaagctaaa
     1201 agcggacaat tacatcaagt cctttcacag tgatcttaac aattagatgg acgcagatgg
     1261 gatggtcgcg tctcccacac gtagttgtga gctaattgag agcaccgctt attacttatt
     1321 agtaccaagc cagttgtacg aggaaccaag caccgtatcg actttttaag ccttggcgtt
     1381 tgtagttttg tggagagaga ttgagatttt accaccgttt cttccctcca acgaccaact
     1441 gctattttga cagccctttc tgctcctttt cctctataaa tagagcagtc tctacaccaa
     1501 cactcttcat actcattcaa gcctgctctg gtagtagcag tactagtagt attggtaata
     1561 gaaatagtag aatatcgaca ctagttgttg cagttgttca ctccttttga gggttgcaga
     1621 gagatggatt gtagtagccc actttctttg tttcatcttt tgttagtatg cacagtgatg
     1681 gttaaatgct gctctgcttc tgatttgcat tactctgatg cactcgaaaa atcaatcctc
     1741 tttttcgaag ggcaaaggtc ggggaaatta cccaccaacc agcgtctcac atggaggggt
     1801 gactctggtt tatcagatgg ctcctcttat catgtatgtg tttttctgat gctataactt
     1861 cactgtccat taactttgtc atatgtatac agagattgaa cttttgattt gttgtatgca
     1921 ggtggattta gtaggcggat actatgatgc aggggataat ctgaagtttg gtctgcctat
     1981 ggccttcact acgacaatgc tggcatgggg tatcattgag ttcggctgct tgatgccgga
     2041 gcaggtagag aatgcaagag cggcccttcg ctggagcacg gattatctac tcaaggcctc
     2101 tacagccact tccaattctt tatatgtcca agtgagcgat cagcttatac tattctcttt
     2161 cccttctcat ctttgggcaa catagagcag gggttacaat tcatcaagat agaaacaaga
     2221 tgtaaacggt aactggaata taatcaacca atccattggt gtctcaagaa ttgagtacat
     2281 gatgcatgta attctttgta agatgacatc cttcttacaa agatgatgag ctttttactt
     2341 gttgaggagg gttgtgggtt atccgaataa ccggtttgag agttgttgca cgtacccatg
     2401 tttcacaacg ttgtcatcgt tgtatgagta ggtgcgcaac tcattaccca tgacttccta
     2461 tctgtggaga gctatcatga atcgcccata atacagaaac actcaagagg ttttgttgca
     2521 gctgtccaat gaccaggtct cttttctttg tgcaattgta gttcacagat ggaaagttag
     2581 gattgcacta gaagtgtggt tcttctacca aggaacaccc attggatggc acaattgcag
     2641 tgggtatcta aattgctgca atgaataata atcgcataga agaccaggca ttctcttttt
     2701 aattgcttca agcccattag aaaagttgct cttgttaatc aagttcataa ttcacgtttt
     2761 gttactagtc aatgttttca tagataatag cgatttcttt cagaaatata tccaatctag
     2821 gaatgtatac atcaccacca tagtttcacc actgccgatt tgccctgctt tttcgatgaa
     2881 tgctttgctt aattgtaatc cgcttggata ttgggaatgc aggtaggaga gcccaacgcg
     2941 gatcaccgat gctgggagag gccagaagac atggatacac ctcgcaacgt ttacaaagtg
     3001 tctacacaga atccaggctc tgatgttgca gcagagacag cagccgcact ggctgctgcc
     3061 tccatcgtat ttggtgactc ggacagttcc tactccacca aattacttca cactgcggtg
     3121 aaagtaatgc tccctcagaa gtgaattcaa tcgggactca gaaattctct tcattgatct
     3181 ttttataagc cactttctct ggaatgcagg tctttgaatt tgcagatcag tacagaggat
     3241 cttacagtga ttccctcggt tcagttgtct gcccatttta ctgctcttat tctgggtaca
     3301 atgtaagaac tgccattaaa cactagcggc attttctttc gaaaactctc atgtccattg
     3361 aactggcttg aaatcaaatt attcctcatg tgaatttgca aaaacaggat gagcttctgt
     3421 ggggtgcatc atggcttcat cgggcatcac aaaatgcgtc gtatatgact tacattcagt
     3481 cgaatggtca cacactaggg gcagatgatg atgattattc tttcagttgg gatgataaac
     3541 gagttggaac caaagttctt ctttccaagg ttttcattct ccacctttct tctagtttct
     3601 caaatgcgag agaagctaat aatttacctt ccatggccca cttccacagg gcttcttgca
     3661 ggacaggatt gaagaactgc agctatataa agtacatact gataattaca tctgctctct
     3721 tatccctgga acatctagtt tccaagccca gtacacacca ggtttgtcgt gtctttgatt
     3781 aacaagaaaa gggtccttag cctcatcaaa ctatttctga ggcatctatt ccttacaagt
     3841 ggagctcgtg agtctgtgat ctggaccaat gatcttatgg gtccactttg ggcatggcct
     3901 aaaataatct cctgggatgg gcattattac ccttgggata caatagaagc ttaagaaaag
     3961 gatgcagtaa aggccatcct tcacatggtc gggaagtagg atcttttgac attgagacat
     4021 tgtggggcat caaggcgtga tgaccgggat ccatacgatg aatagtgtag accatgaaat
     4081 gggttcattt tgcagtatag attcttgttt cctttgaatc aagcattcca taaactattt
     4141 atctgcatcg actgacaggt gggcttctct acaaaggaag tgcgagcaat ctacagtatg
     4201 taacatccac agcttttctc ctactaacat atgctaatta tctgaactct tccggaggac
     4261 atgcttcatg tggtaccact actgttacag ccaagaacct catctcactc gcaaagaaac
     4321 aggtacctat atttagtgat atagactatc agcaactcaa ccattcatct ccctgtatgc
     4381 tcacagactc gaatggattt attatcaggt tgactacata ttaggtcaga acccggccaa
     4441 gatgtcatac atggtaggat tcggagagag gtatccacag catgtccatc acagaggctc
     4501 ctcacttcca tctgtacagg tgcaccctaa ttccataccc tgcaatgctg gatttcagta
     4561 cctgtattct agcccaccca acccaaatat cctagtgggt gccatattgg gtggtccaga
     4621 taacagagac agtttctcag atgaccggaa caattatcag cagtcagaac cagccactta
     4681 cataaatgcc ccacttgttg gtgctctagc attcttcgca gcaaacccag tgacggagtg
     4741 acagagattg ccctctcttt tacgtaaact gatagaaata ataaactatg gggtttcttt
     4801 gtaatttcct atgatggacc tagcagcaaa ccaggtgatg gagtcacaga gattgtcctc
     4861 tcttttatgt aaactgttag aaataataaa ctatgggtgt ttctttgtaa tttcctatga
     4921 tgggcctagc tgtaatattt gaaagtgtgt ttgaatatat tgagttcagc tctcgattag
     4981 ggcctgagct cttttgccct ctctcaatct cacgaatctc tctcaatctc acaaacaaca
     5041 tggtatcaga gcattcgatc gtcgcatgaa ttttcatttc t
//