From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Escherichia coli restriction-modification enzyme type I M subunit
Date: 31 Dec 92 01:44:24 GMT
Distribution: bionet
Checksum: 41305 4

LOCUS       ECOHSDMA     1470 bp ds-DNA             BCT       18-DEC-1992
DEFINITION  Escherichia coli restriction-modification enzyme type I M subunit
            (hsdM) gene, complete cds.
ACCESSION   L02505
KEYWORDS    hsdM gene; restriction-modification enzyme type I M subunit;
            restriction-modification system type I M subunit.
SOURCE      Escherichia coli (strain 15T) DNA.
  ORGANISM  Escherichia coli
            Prokaryotae; Gram-negative facultatively anaerobic rods;
            Enterobacteriaceae.
REFERENCE   1  (bases 1 to 1470)
  AUTHORS   Sharp,P.M., Kelleher,J.E., Daniel,A.S., Cowan,G.M. and Murray,N.E.
  TITLE     Roles of selection and recombination in the evolution of type I
            restriction-modification systems in enterobacteria
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 89, 9836-9840 (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     CDS             1..1470
                     /gene="hsdM"
                     /citation=[1]
                     /note="homologue to X06545, and to X13145; putative"
                     /product="restriction-modification enzyme type I M
                     subunit"
                     /codon_start=1
                     /translation="MSISSVIKSLQDIMRKDAGVDGDAHGLGQLSWLLFLKIFDAQEE
                     ALELEQDNYQYPIPQRYLWRSWAANAQGITGDSLLEFVNDDLFPALKNLTAPIDKNPR
                     GYVVKQAFSDAYNYMKNGTLLRQVINKLNEIDFTSASERHLFGDIYEQILKDLQSAGN
                     AGEFYTPRAVTRFMVDRVDPKLGESIMDPACGTGGFLACAFDHVKNKYVKSVADHQTL
                     QQQIHGVEKKQLPHLLATTNMLLHGIEVPVQIRHDNTLNKPLSSWDEQLDVIVTNPPF
                     GGTEEDGIEKNFPAEMQTRETADLFLQLIVEVLAKNGRAAVVLPDGTLFGEGVKTKIK
                     KLLTEECNLHTIVRLPNGVFNPYTGIKTNLLFFTKGQPTKEIWFYEHPYPAGVKNYSK
                     TKPMKFEEFQAEIDWWGNEADGFASRVENEQAWKVSIDDVIARNFNLDIKNPHQAETV
                     SHDPDELLAQYAKQQAEIQTLRNQLRDILGAALSVKEVN"
BASE COUNT      398 a    359 c    381 g    332 t
ORIGIN      
        1 atgtctatca gttcagtaat caaatcatta caagatatta tgcgcaaaga tgccggtgtg
       61 gacggcgatg cgcacggtct cggtcagctc tcttggctgc tgtttttgaa aatcttcgat
      121 gcccaggaag aggcgctgga actggagcag gataactatc aatatccgat cccacagcgt
      181 tatttgtggc gcagttgggc cgcaaacgct cagggcatta ccggtgattc cctgctggaa
      241 ttcgttaatg atgatctgtt cccggcgttg aaaaacctca ctgcgcctat cgataaaaac
      301 ccacgcggct acgtggtgaa gcaggcgttc agcgatgcct ataactatat gaaaaacggt
      361 acgctactgc gtcaggtgat caacaagctg aacgaaattg actttaccag cgccagcgaa
      421 cgccatctgt ttggtgatat ttacgaacaa atccttaaag atctgcaatc tgcgggcaat
      481 gctggcgaat tctatactcc acgcgccgtc actcgcttta tggtggatcg cgttgatccg
      541 aaactcggcg aatccattat ggacccggcc tgcggtacgg gcggttttct tgcctgcgca
      601 tttgatcatg taaagaacaa atacgtgaag agcgtcgccg atcatcagac gctgcaacaa
      661 cagatccacg gtgttgagaa aaaacagctt ccgcacctgc tggcgaccac caatatgctg
      721 ctacacggca ttgaagtgcc agtgcaaatt cgtcacgaca acactctgaa caaaccgctt
      781 tcctcctggg atgagcaact ggatgtcatt gttaccaacc cgccatttgg tggcacggaa
      841 gaagacggta ttgagaagaa ctttccggca gagatgcaaa cccgcgaaac ggcggatctg
      901 ttcctgcaac tgattgtgga agtactggcg aaaaacggtc gtgcggcggt ggtattgccg
      961 gatggcacgc tatttggcga aggcgttaaa accaaaatca aaaagctact taccgaagag
     1021 tgcaacctgc ataccatcgt gcgcttaccg aatggtgtgt ttaaccccta taccggcatt
     1081 aaaaccaacc tgttgttctt taccaaaggc cagccaacca aagagatttg gttctatgag
     1141 catccgtatc cggcgggcgt gaaaaactac agcaaaacca agccgatgaa gtttgaagag
     1201 tttcaggcgg agatcgactg gtggggtaac gaggccgatg gttttgccag ccgcgtcgag
     1261 aatgagcaag cgtggaaagt cagcattgat gacgtgattg cgcgtaactt caatctggat
     1321 attaaaaacc cacatcaggc ggaaaccgta agccatgatc cggacgaact gttagcgcag
     1381 tatgcaaagc aacaggcgga gatccagacg ctgcgtaatc aactgcgcga tattctcggc
     1441 gctgcgctgt ctgtcaagga ggttaactga
//