From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Clostridium thermocellum celX gene, 3' end and endo-beta-1,
Date: 7 Apr 92 10:01:38 GMT
Distribution: bionet
Checksum: 43025     8

LOCUS       CLOCELEX     3471 bp ds-DNA             BCT       07-APR-1992
DEFINITION  Clostridium thermocellum celX gene, 3' end and endo-beta-1,
            4-glucanase (celE) gene, complete cds.
ACCESSION   M22759
KEYWORDS    endo-beta-1,4-glucanase; endoglucanase; xylan-hydrolysing activity.
SOURCE      Clostridium thermocellum DNA.
  ORGANISM  Clostridium thermocellum
            Prokaryota; Bacteria; Firmicutes; Endospore-forming rods and cocci;
            Bacillaceae.
REFERENCE   1  (bases 1 to 3471)
  AUTHORS   Hall,J., Hazlewood,G.P., Barker,P.J. and Gilbert,H.J.
  TITLE     Conserved reiterated domains in Clostridium thermocellum
            endoglucanases are not essential for catalytic activity
  JOURNAL   Gene 69, 29-38 (1988)
  STANDARD  simple staff_entry
FEATURES             Location/Qualifiers
     CDS             <1..676
                     /note="product homologous to other C.thermocellum
                     endoglucanases"
                     /gene="celX"
                     /codon_start=2
                     /translation="IMKVTQDGSIPQIASNINNWLNTHNPDVVFLWIGGNDLLLSGNV
                     NATGLSNLIDQIFTVKPNVTLFVADYYPWPEAVKQYNAVIPGIVQQKANAGKKVYFVK
                     LSEIQFDRNTDISWDGLHLSEIGYTKIANIWYKYTIDILKALAGQTQPTPSPSPTPTD
                     SPLVKKGDVNLDGQVNSTDFSLLKRYILKVVDINSINVTNADMNNDGNINSTDISILK
                     RILLRN"
     -10_signal      897..902
                     /gene="celE"
     CDS             1027..3471
                     /product="endo-beta-1,4-glucanase"
                     /gene="celE"
                     /codon_start=1
                     /translation="MKKIVSLVCVLVMLVSILGSFSVVAASPVKGFQVSGTKLLDASG
                     NELVMRGMRDISAIDLVKEIKIGWNLGNTLDAPTETAWGNPRTTKAMIEKVREMGFNA
                     VRVPVTWDTHIGPAPDYKIDEAWLNRVEEVVNYVLDCGMYAIINLHHDNTWIIPTYAN
                     EQRSKEKLVKVWEQIATRFKDYDDHLLFETMNEPREVGSPMEWMGGTYENRDVINRFN
                     LAVVNTIRASGGNNDKRFILVPTNAATGLDVALNDLVIPNNDSRVIVSIHAYSPYFFA
                     MDVNGTSYWGSDYDKASLTSELDAIYNRFVKNGRAVIIGEFGTIDKNNLSSRVAHAEH
                     YAREAVSRGIAVFWWDNGYYNPGDAETYALLNRKTLSWYYPEIVQALMRGAGVEPLVS
                     PTPTPTLMPTPSPTVTANILYGDVNGDGKINSTDCTMLKRYILRGIEEFPSPSGIIAA
                     DVNADLKINSTDLVLMKKYLLRSIDKFPAEDSQTPDEDNPGILYNGRFDFSDPNGPKC
                     AWSGSNVELNFYGTEASVTIKSGGENWFQAIVDGNPLPPFSVNATTSTVKLVSGLAEG
                     AHHLVLWKRTEASLGEVQFLGFDFGSGKLLAAPKPLERKIEFIGDSITCAYGNEGTSK
                     EQSFTPKNENSYMSYAAITARNLNASANMIAWSGIGLTMNYGGAPGPLIMDRYPYTLP
                     YSGVRWDFSKYVPQVVVINLGTNDFSTSFADKTKFVTAYKNLISEVRRNYPDAHIFCC
                     VGPMLWGTGLDLCRSYVTEVVNDCNRSGDLKVYFVEFPQQDGSTGYGEDWHPSIATHQ
                     LMAERLTAEIKNKLGW"
     sig_peptide     1027..1128
                     /gene="celE"
                     /codon_start=1
     mat_peptide     1129..3468
                     /product="endo-beta-1,4-glucanase"
                     /gene="celE"
                     /codon_start=1
BASE COUNT     1074 a    632 c    811 g    954 t
ORIGIN
        1 gatcatgaag gtcactcagg atggatcgat tccccagata gcaagcaata tcaacaactg
       61 gcttaatacc cataacccgg atgtggtatt tctgtggatt ggaggaaatg acctgctttt
      121 gagcggaaac gtgaatgcaa caggccttag taatcttata gaccagattt tcacagtgaa
      181 acccaatgta acactgtttg tggccgatta ttatccgtgg cctgaagcgg tcaagcaata
      241 caatgcggtg attccgggaa tagttcaaca gaaggccaat gccggcaaga aagtttattt
      301 tgtaaagctt agtgagattc agtttgacag gaacaccgat atttcatggg atggtttgca
      361 cttgagcgaa ataggataca caaagattgc aaatatttgg tacaagtata cgattgacat
      421 actaaaagct ttggcaggac aaacgcagcc aactccaagt ccgtctccga ctcccacaga
      481 ttctcctctg gttaaaaaag gtgatgttaa tttggacggt caggtcaatt cgacagattt
      541 cagccttttg aaaagatata tactgaaagt tgtggatata aattcaataa atgtgacaaa
      601 tgctgatatg aacaatgatg gcaatatcaa ctctacagac atttcaatac taaagagaat
      661 acttcttaga aattagaaaa tatattgtgg atttttaaag tttttaataa acggatatat
      721 aaaagaagct ccaaattctt tggagcttct tttatatatc ccgggtttcc aacaaagtgc
      781 aacctttttt cagcagaggt tactatgttt ttaattattt ttttgctttg cgctgtccaa
      841 aagaaaatga tttttcgaat taatataata aacagtaaca ttaccgttta gtttgttata
      901 atgttttatt tcaagccaaa taggaagggg gagctgtaag aataaagttg attgatgcaa
      961 gaaaagagga tttaaagtaa aagattattg ttttttgtaa aggatttttg taaaggagag
     1021 ggtaatgtga aaaaaattgt ttctttggtt tgtgtgcttg tgatgctggt aagcatctta
     1081 ggctcgtttt cagtcgtagc ggcatcaccg gtaaaaggct ttcaggtatc gggaacaaag
     1141 cttttggatg caagcggaaa cgagcttgta atgaggggca tgcgtgatat ttcagcaata
     1201 gatttggtta aagaaataaa aatcggatgg aatttgggaa atactttgga tgctcctaca
     1261 gagactgcct ggggaaatcc aaggacaacc aaggcaatga tagaaaaggt aagggaaatg
     1321 ggctttaatg ccgtcagagt gcctgttacc tgggatacgc acatcggacc tgctccggac
     1381 tataaaattg acgaagcatg gctgaacaga gttgaggaag tggtaaacta tgttcttgac
     1441 tgcggtatgt acgcgatcat aaatcttcac catgacaata catggattat acctacatat
     1501 gccaatgagc aaaggagtaa agaaaaactt gtaaaagttt gggaacaaat agcaacccgt
     1561 tttaaagatt atgacgacca tttgttgttt gagacaatga acgaaccgag agaagtaggt
     1621 tcacctatgg aatggatggg cggaacgtat gaaaaccgag atgtgataaa cagatttaat
     1681 ttggcggttg ttaataccat cagagcaagc ggcggaaata acgataaaag attcatactg
     1741 gttccgacca atgcggcaac cggcctggat gttgcattaa acgaccttgt cattccgaac
     1801 aatgacagca gagtcatagt atccatacat gcttattcac cgtatttctt tgctatggat
     1861 gtcaacggaa cttcatattg gggaagtgac tatgacaagg cttctcttac aagtgaactt
     1921 gatgctattt acaacagatt tgtgaaaaac ggaagggctg taattatcgg agaattcgga
     1981 accattgaca agaacaacct gtcttcaagg gtggctcatg ccgagcacta tgcaagagaa
     2041 gcagtttcaa gaggaattgc tgttttctgg tgggataacg gctattacaa tccgggtgat
     2101 gcagagactt atgcattgct gaacagaaaa actctctcat ggtattatcc tgaaattgtc
     2161 caggctctta tgagaggtgc cggcgttgaa cctttagttt caccgactcc tacacctaca
     2221 ttaatgccga ccccctcgcc cacggtgaca gcaaatattt tgtacggtga cgtaaacggg
     2281 gacggaaaaa taaattctac agactgtaca atgctaaaga gatatatttt gcgtggcata
     2341 gaagaattcc caagtcctag cggaattata gccgctgacg taaatgcgga tctgaaaatc
     2401 aattccaccg acttggtatt gatgaaaaaa tatctactgc gctcaataga caaatttcct
     2461 gcggaggatt ctcaaacacc tgatgaagac aatccgggca ttttgtataa cggaagattc
     2521 gatttttcag atccgaacgg tccgaaatgc gcctggtccg gcagcaatgt tgagctgaat
     2581 ttttacggca cggaagcaag tgtgactatc aaatccggcg gtgagaactg gttccaggct
     2641 attgtagacg gcaatcctct tcctcctttt tcggttaacg ctactacctc taccgtaaag
     2701 cttgtaagcg gtcttgcaga aggagctcat catcttgtat tgtggaagag gacagaggca
     2761 tccttgggag aagttcagtt ccttgggttt gattttggtt caggaaagct tcttgccgca
     2821 ccgaagcctt tggaaagaaa gattgagttt atcggagact ccatcacatg tgcatacgga
     2881 aatgaaggaa caagcaagga gcagtctttt acaccgaaaa atgaaaacag ctatatgtct
     2941 tatgcggcaa ttacagcccg taatttgaat gcaagtgcaa atatgattgc gtggtccgga
     3001 atcggactta ccatgaacta cggcggagcc cccggacctc ttataatgga ccgttatcct
     3061 tatacccttc cttacagcgg agtcagatgg gattttagca aatatgtgcc tcaggttgtt
     3121 gtaatcaatc ttggtaccaa tgatttttct acatcatttg cagataaaac aaagtttgta
     3181 acggcatata aaaaccttat aagtgaagtt cgcaggaact atccggatgc ccatatattc
     3241 tgctgtgtcg gtccgatgct ttggggaacg ggcctggatt tgtgccgcag ttatgttacg
     3301 gaagttgtaa atgattgtaa cagaagcggg gatttaaagg tgtattttgt tgagtttccg
     3361 cagcaggacg gaagcaccgg atacggagaa gactggcatc caagtattgc cacccaccag
     3421 ctgatggctg agcggcttac tgcggaaata aaaaacaagc ttggatggta a
//