From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: A.niger pelB gene for pectin lyase B
Date: 4 Sep 92 10:02:19 GMT
Distribution: bionet
Checksum: 55344     6

LOCUS       ASNPELB      2774 bp ds-DNA             PLN       04-SEP-1992
DEFINITION  A.niger pelB gene for pectin lyase B
ACCESSION   X65552
KEYWORDS    pectin lyase; pelB gene.
SOURCE      Aspergillus niger DNA.
  ORGANISM  Aspergillus niger
            Eukaryota; Fungi; Eumycota; Ascomycotina; Plectomycetes;
            Eurotiales; Trichocomaceae.
REFERENCE   1  (bases 1 to 2774)
  AUTHORS   Kusters-Van Someren,M.A., Flipphi,M., de Graaff,L.,
            van den Broeck,H., Kester,H.C., Hinnen,A. and Visser,J.
  TITLE     Characterization of the aspergillus niger pelB gene: structure and
            regulation of expression
  JOURNAL   Mol. Gen. Genet. 234, 113-120 (1992)
  STANDARD  full automatic
COMMENT     *source: strain=N400;
            From EMBL    entry ANPELB;  dated 10-AUG-1992.
FEATURES             Location/Qualifiers
     exon            <1134..1337
                     /number=1
     CDS             join(1134..1337,1400..1540,1600..1725,1783..2112,
                     2170..2505)
                     /product="pectin lyase B"
                     /gene="pelB"
                     /codon_start=1
                     /translation="MHYKLLFAAAAASLASAVSAAGVVGAAEGFAHGVTGGGSASPVY
                     PTTTDELVSYLGDNEPRVIILDRTFDFTGTEGTETTTGCAPWGTASQCQVAINLHSWC
                     DNYQASAPKVSVTDKAGILPITVNSNKSIVGQGTKGVIKGKGLRVVSGAKNVIIQNIA
                     VTDINPKYVWGGDAITVDDSDLVWIDHVTTARIGRQHIVLGTSADNRVTISYSLIDGR
                     SDYSATCNGHHYWGVYLDGSNDMVTLKGNYFYNLSGRMPKVQGNTLLHAVNNLFHNFD
                     GHAFEIGTGGYVLAEGNVFQDVNIVVETPISGQLFSSPDANTNQQCASVFGRSCQLNA
                     FGNSGSMSGSDTSIISKFAGKTIAAAHPPGNIAQWTMKNAGQGK"
     TATA_signal     951..958
     intron          1338..1399
                     /number=1
     exon            1400..1540
                     /number=2
     intron          1541..1599
                     /number=2
     exon            1600..1725
                     /number=3
     intron          1726..1782
                     /number=3
     exon            1783..2112
                     /number=4
     intron          2113..2169
                     /number=4
     exon            2170..>2505
                     /number=5
BASE COUNT      653 a    785 c    673 g    663 t
ORIGIN
        1 ctcgagtcca gtagttagta gttgcggata acaaggatag atcatcccct ttggttatgt
       61 gtgagtctga ccaaggaagc attgggggag agactattgg gtcaatggca ctcacgggaa
      121 aaagaggcga aagggatggg agaggtgatg cctgaggccg catgaatata tcatgtgaga
      181 gcaaagaaga tcaaacaaac aatacaaacc ccagcgtgac ccctcactgc cacgtgattt
      241 cccagctaat cctcttcggg gtgttttccc ccccaatcgg ggcttcttgg caaccactaa
      301 tttccataat ttccactgga ctcttgaacg gttcttgcaa tgtctcactg tcagactgag
      361 agtaccactt ttctccctct accaagcctt gaaataggtc agggccgttc atgggaccct
      421 gacctccacc ccttcggaag ccagccggat tgcttctaag acatcctgaa gtgggccacc
      481 atccggctct caccattcct atgctctgcc aagcgactcg acctcgttct tcaccggagt
      541 gcccgggtcc gccctgaact caaggtctgc atgggaagag atttccggct gtttccccca
      601 acggcgtcta ggcagatagg ctaggcgttg tgttcctggc ttgatcagca aggtttatca
      661 tagtcctcat gactcgtaaa acccaagaag ataaaatgaa gatgttgggt ccgcgagtcc
      721 ggtgatgtct gggcagcagt agtaacactc taaaatataa gccaccacac ccactgacag
      781 gttaatctcc ggtagaagaa ccaagaccta cctgccaagc cacgttgatt gggttctcat
      841 ccatttcagg tcctgtcccc ggtcccctgg tgtcattcca gaacatggtc aaggaaatca
      901 aacggccact gtctgactgc cagctccacc atcatgtaat ccggcttctc tatataaact
      961 tgggaatgtt cccctctcct gtgccaccca gaagaagaca tcaccttcct tgcttctaca
     1021 agcctgtcac tctttccagg ccgtcgttct ttgatatttc tcactagact ttcattctct
     1081 tgaatatttt ctttttgttt tccctcgtat tttctgtgct ttgagagccc aacatgcatt
     1141 acaaactgct ttttgctgct gccgcagcat ccttggccag cgctgtcagt gccgccggtg
     1201 ttgttggtgc cgccgagggt ttcgcccatg gtgtcactgg cggtggcagc gcttcccccg
     1261 tctatcctac gactactgat gagctggtct cttacctcgg agataacgag ccccgggtga
     1321 ttatcctgga tagaacgtag gtcgatcctc aattggaaat ggatgggtag acgtgggcct
     1381 aacaggttga taatccagct tcgacttcac cggcactgag ggtactgaaa ctaccaccgg
     1441 atgtgccccc tggggaactg cttcccaatg ccaggtggcc atcaacctgc acagctggtg
     1501 tgacaactac caggctagcg cccccaaggt atccgtgact tagtatgttg tccccgttcg
     1561 attggtgacc ctgtctttcc atgctgatag ccgtatagtg ataaggcggg tatcctcccc
     1621 attacggtca actccaacaa aagtatcgtt ggtcagggca ccaagggagt catcaagggc
     1681 aagggtctcc gtgtggtcag cggtgccaag aacgtcatca tccagtgagt gcagaacatc
     1741 ggtcttagga tgtgaaaaaa ccctttgcta ataaaggcca ggaacattgc cgttaccgac
     1801 atcaacccca agtatgtctg gggtggtgat gccattactg tcgatgactc tgatctggtc
     1861 tggatcgacc atgtgaccac tgctcgcatt ggtcgccagc acatcgttct gggaaccagc
     1921 gccgacaacc gcgtcaccat ctcctactcc ctcatcgatg gtcgctccga ctactctgcc
     1981 acctgcaacg gccaccacta ctggggcgtg tacctggacg gcagcaacga catggtcacc
     2041 cttaagggca actacttcta caacctgagc ggccgcatgc ccaaggttca gggtaacact
     2101 ctgctgcacg ccgtatgtcg ccttgagcct cctatatgat ttgcacatta ctgacaagtc
     2161 tgactacagg tgaacaacct cttccacaac tttgacggcc acgccttcga aatcggcact
     2221 ggtggctacg tcctggccga gggtaacgtc ttccaggacg ttaacattgt ggtggagacg
     2281 cccatcagcg gccagctctt cagctccccc gacgccaaca ccaaccagca gtgcgcctcc
     2341 gtcttcggtc gttcctgcca gctcaacgcc ttcggcaact ccggctcgat gtccggatcg
     2401 gacaccagca tcatcagcaa gttcgctggc aagaccattg ctgcggctca ccccccgggt
     2461 aacattgccc agtggaccat gaagaacgct ggccagggca aataaggttt tacagcagaa
     2521 caattctgtg aaggaggctg gccaaagccc agatgaggat agagggtacc tgcttcgaca
     2581 tctatagttc aatagtccag gattatagac gaatgattat tgctccagaa tggtgaagta
     2641 tttctagcag accatgacgc gtacgcaaca tagaccgctc catgttactg actgcgactg
     2701 tatttggagg attgaggaaa ggtagataaa agtatatact acaaaaccgc cataacagca
     2761 accgacgcct cgag
//