From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: E.coli genes for H-ns thymidine kinase alcohol dehydrogenase
Date: 15 Aug 92 10:01:40 GMT
Distribution: bionet
Checksum: 48380     7

LOCUS       ECOTKAD      3060 bp ds-DNA             BCT       15-AUG-1992
DEFINITION  E.coli genes for H-ns thymidine kinase alcohol dehydrogenase
ACCESSION   X67326
KEYWORDS    adhE gene; alcohol dehydrogenase; hns gene; tdk gene;
            thymidine kinase.
SOURCE      Escherichia coli DNA.
  ORGANISM  Escherichia coli
            Prokaryotae; Gram-negative facultatively anaerobic rods;
            Enterobacteriaceae.
REFERENCE   1  (bases 1 to 3060)
  AUTHORS   Danchin,A.
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
REFERENCE   2  (sites)
  AUTHORS   Krin,E. and Danchin,A.
  TITLE     Filling the gap between hns and adhE
  JOURNAL   Unpublished (1992)
  STANDARD  full staff_review
COMMENT     *source: strain=K12;
            From EMBL    entry ECTKAD;  dated 28-JUL-1992.
FEATURES             Location/Qualifiers
     CDS             complement(<1..405)
                     /product="H-ns"
                     /gene="hns"
                     /codon_start=1
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLI"
     RBS             complement(414..417)
                     /gene="hns"
     -10_signal      complement(447..453)
                     /gene="hns"
     -35_signal      complement(471..476)
                     /gene="hns"
     -35_signal      937..942
                     /gene="tdk"
     -10_signal      959..964
                     /gene="tdk"
     RBS             995..998
                     /gene="tdk"
     CDS             1010..1627
                     /product="thymidine kinase"
                     /gene="tdk"
                     /evidence=EXPERIMENTAL
                     /codon_start=1
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVDSLTAIQERHRHD"
     CDS             complement(1909..2499)
                     /note="orf"
                     /codon_start=1
                     /translation="MALPPDRTDRGAERYLKDDWHGLQLFAIDGAQFRTPDKPELREY
                     YGSANTSTKRQNAYPVMRLVALMNLGSHILLNAVTAPYRQSETVLAHSMLATIPDNSI
                     TLFDKLFYSEDLLLTLNQKGCNRHWLLPAWKNIASEMIELGNTASPGTIPKRLEHLRG
                     ALEVVFITKRPRPSRPRSVKISKTRYPVKHSAAPLK"
     misc_feature    2809..2838
                     /note="palindrome"
     misc_feature    2850..2875
                     /note="palindrome"
     CDS             complement(2929..>3060)
                     /product="alcoholdehydrogenase"
                     /gene="adhE"
                     /codon_start=1
                     /translation="PLISELKQILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
BASE COUNT      765 a    686 c    751 g    858 t
ORIGIN
        1 gatcaggaaa tcgtcgaggg atttaccttg ctcatccatt gcttttttga ttacagctgg
       61 agtacggcct tggccagtcc aggttttagt ttcgccgttt tcgtcaacgt agctatattt
      121 tgccggacgc tgagcacgtt tagctttggt gccagattta acggcagcaa ggctattcag
      181 cagttcgttc gggtcaatac cgtcagcgat cagcatttcg cgatattgct gcagtttacg
      241 agtgcgctct tcaacttcag cagcagccgc gctttcttct tcgcgacgtt cgttaacaac
      301 aacttctaat ttttccagca tttcttccag cgtttcaagt gtacattctc ttgcctgcgc
      361 acgaagagta cggatgttgt tcagaatttt aagtgcttcg ctcattgtag taatctcaaa
      421 cttatattgg ggtggtttgt tgaggtaata atagagcctt aaattcagtt gtgcaatagc
      481 caggaatgta aggaattcaa aattgttctt tattttgtgc cgccaataaa tatcttttca
      541 taaaattagc cagaaaagac gcggcatata gccctattta caccgatgat ttcgcagcac
      601 gtgaggttaa aacttcctga ttcatgtcac attttatggg gagattatcg taggctgacg
      661 acctttcagt cttctgtatt agttgtgttt acgagaattc cctattaagc gaatgatgaa
      721 aagtagaaca gtcgcaataa gagcatggac ttagtattgc actatctcct ggaggtcaac
      781 agagggctat tacttgcgca acaggttaaa gattgtgaat agttaccagc agtcatttac
      841 ccgcttataa caagcgaggc agttgtaatg atagctcaga aggattatgc aaggcttcgt
      901 aagggagaac gcatataccc acttctgtgc atactgttga gctgaaaaac tgacgaatta
      961 tgataaactc cagccaactt tatttcatat cattgagggc ctgtggctga tggcacagct
     1021 atatttctac tattccgcaa tgaatgcggg taagtctaca gcattgttgc aatcttcata
     1081 caattaccag gaacgcggca tgcgcactgt cgtatatacg gcagaaattg atgatcgctt
     1141 tggtgccggg aaagtcagtt cgcgtatagg tttgtcatcg cctgcaaaat tatttaacca
     1201 aaattcatca ttatttgatg agattcgtgc ggaacatgaa cagcaggcaa ttcattgcgt
     1261 actggttgat gaatgccagt ttttaaccag acaacaagta tatgaattat cggaggttgt
     1321 cgatcaactc gatatacccg tactttgtta tggtttacgt accgattttc gaggtgaatt
     1381 atttattggc agccaatact tactggcatg gtccgacaaa ctggttgaat taaaaaccat
     1441 ctgtttttgt ggccgtaaag caagcatggt gctgcgtctt gatcaagcag gcagacctta
     1501 taacgaaggt gagcaggtgg taattggtgg taatgaacga tacgtttctg tatgccgtaa
     1561 acactataaa gaggcgttac aagtcgactc attaacggct attcaggaaa ggcatcgcca
     1621 cgattaataa gaatttcttt actgacaggg tgagcagggc acttttatcc tgtcagttcg
     1681 ttttacgcac ttcttccggg ctatataccc ttctcggcag ttttttaacg ccgctatacg
     1741 cctcacaggg ctcttaagca ccgacgttga cttgtgacct gtaaagtaca atatccctgt
     1801 gtttaggcgt tatacatcgt cgcaaatatg atgaaggcta atgctgtcgg tttatggaaa
     1861 agttgctttg ggtaaacaaa aaatacggcc ccagaagggc aatgccgttc acttaagagg
     1921 agcggcacta tgtttcacag gataacgggt ttttgatatc ttaaccgacc tcggccttga
     1981 tggtcggggg cgttttgtta tgaacaccac ttccagagca ccccgaagat gctccagtcg
     2041 tttcgggatg gtccctggtg acgccgtgtt tcccagctct atcatttctg atgcgatatt
     2101 cttccacgca ggcagtagcc agtggcggtt acaacccttc tggttcagcg tcagcagcag
     2161 gtcttcgctg taaaaaagtt tatcaaacaa cgtaatagag ttatccggga tggtggcgag
     2221 catggagtgg gccagcacag tttcgctctg ccggtaaggt gcggtcacgg cattcagcag
     2281 aatgtgactt cccaggttca ttaaggccac cagacgcata accgggtagg cgttctgccg
     2341 cttagtggat gtgttggcag acccataata ttcacgcagc tcgggtttat caggtgtcct
     2401 gaactgtgcg ccatcaatgg caaaaagttg caggccgtgc cagtcatcct tcaggtaacg
     2461 ttccgcgccc ctgtctgtgc ggtctggcgg aagagccatt ccactggggc ggcccccacg
     2521 cgctgacgcg cctgggtgac agcgctgcgg gccagcaggt tcatccccgc ttcgccatcc
     2581 gcgctcaggt tcagacggcg aacaacatcg gtaattggct cattgcacca ccatccagat
     2641 aaccatgtcc cccggtaaac gacggcggcg aacggtcgca tgagcagaaa gcgtcaggca
     2701 gtgttgtatc cactcggtgg gaaggtgttc tgcaaatagt tgtgcagagg gcggaggcat
     2761 aagcggatgg tcactgaaat cgagcagatc attgagaagt ggcataagaa aacggctccc
     2821 tgttgtggaa gccgttatag tgcctcagtt taaggatcgg tcaactaatc cttaactgat
     2881 cggcattgcc cagaaggggc cgtttatgtt gccagacagc gctactgatt aagcggattt
     2941 tttcgctttt ttctcagctt tagccggagc agcttctttc ttcgctgcag tttcaccttc
     3001 tacataatca cgaccgtagt aggtatccag cagaatctgt ttcagctcgg agatcagcgg
//