From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Bacillus stearothermophilus gene for aminopeptidase II, complete
Date: 3 Jan 93 22:11:34 GMT
Distribution: bionet
Checksum: 17018 8

LOCUS       BACAMINOII   4548 bp ds-DNA             BCT       23-DEC-1992
DEFINITION  Bacillus stearothermophilus gene for aminopeptidase II, complete
            cds.
ACCESSION   D13385
KEYWORDS    aminopeptidase II.
SOURCE      Bacillus stearothermophilus (strain NCIB 8924) DNA.
  ORGANISM  Bacillus stearothermophilus
            Prokaryotae; Firmicutes; Endospore-forming rods and cocci;
            Bacillaceae.
REFERENCE   1  (bases 1 to 4548)
  AUTHORS   Motoshima,H., Minagawa,E., Tsukasaki,F. and Kaminogawa,S.
  TITLE     Molecular cloning and nucleotide sequence of the aminopeptidase II
            gene from Bacillus stearothermophilius NCIB8924 and
            characterization of expressed enzyme
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
COMMENT     Submitted (09-Oct-1992) to DDBJ by: Hidemasa Motoshima Research
            Center Yotsuba Milk Products 465-1 Wattsu, Hiroshima-cho
            Sapporo-gun Hokkaido 061-12 Japan Phone:  011-377-5561 Fax:
            011-377-4393.
FEATURES             Location/Qualifiers
     CDS             2607..3848
                     /EC_number="3.4.11.12"
                     /gene="aminopeptidase II"
                     /evidence="EXPERIMENTAL"
                     /product="aminopeptidase II"
                     /codon_start=1
                     /translation="MNRWEKELDKYAELAVKVGVNIQPGQTLFVNAPLEAAPLVRKIA
                     KTAYETGAKHVYFEWNDEALTYIKFHHAPEEAFSEYPMLRARAMEELAEQGAAFLSIH
                     APNPDLLKDVDPKRIATANKTAAQALANYRSAIMADRNCWSLISVPTPAWAQKVFGDL
                     RDEEAIDKLWEAIFRITRIDQDDPIAAWREHNDRLARIVDYLNNKQYKQLVYEAPGPI
                     FTVELVDGHVWHGGAATSQSGVRFNPNIPTEEVFTMPHKDGVNGTVRNTKPLNYNGNV
                     IDGFTLTFKDGQVVDFSAEQGYETLKHLLDTDDGARRLGEVALVPHQSPVSLSNLIFY
                     NTLFDENAACHLALGKAYPTNIENGASLSKEELDRRGVNDSLVHVDFMIGSADLNIDG
                     VTKDGKREPIFRSGNWAFELA"
BASE COUNT     1148 a   1310 c   1138 g    952 t
ORIGIN      
        1 gaattcgata taacgcggat aacttgaacg ccgccatatg cactttcgcc cattcgatca
       61 aactcagacg gcgccacctt tcctttttcc accccttttt cccgcaaata gcgggcaaaa
      121 cggtgaacgt gatcgagcaa ggttccccac gtgatcacct tgttatagga aaatgtacgc
      181 cggctcgttt tccttcgttc gccgcgatgg cgcaaatagc catagagcgg ctgttcgccg
      241 agctcatagc ggagttcctt cggcagcccg ctcggccagt atgggcgtgt ttcattcatt
      301 cgtttcacca tcccttataa aaaaatactg aatggttagt atgttacctt tcagaaaacg
      361 atttcaaata cttgttcatg tctcctgctt catccatatg catatcattt cgccgtcatt
      421 cgccaaattc ctttctcgaa cagccaatca ttttatccga cgcggcaaac gaccaccccc
      481 ttccacgttg gagaaggagc ggccgttcgg aaaaggagga agcctgaagc agcgcatgcg
      541 gcttggttac ggtcattgat gcgatgaggc cggtcggtgt ggacgatgcg ttttatccct
      601 ccaccagtgg cggagatatg gcagcaccca atgatccaag ccatatcgtc ccgcattggc
      661 cccggcgacg aggagaaaga cggtaagaag caccatttgc gggttcgtgc tcgtcgtgcc
      721 tgagaacatg aaggcgaagt tcatcacagc tcccatgagg gcggcaaacg tcgtaaacaa
      781 tccgagaatg agcgcgatcc cgacgagaaa ttcaccccat ggcacaagaa cgttgaacaa
      841 tccgacattc ggcaacgcca cgtgttcgat aaacgccgcc caccagcttt gcacggccgg
      901 atgttcccca gcggctttcg ccaacgctcc ttttaaaaat ccggtggcgt caaacccttg
      961 cgtgattttt tcccaccctg ccgtcatcca ttcatatcct aaatacaagc ggaatacgac
     1021 catcaacacc gatgccgtga cgtgttcgcg caaccatttg acaaccatcg tgtttctccc
     1081 ctttcgtttt ggaagatgtt ccttacactt ttatccttaa tcgttttttc ctggcggcgc
     1141 agagttgctc actatttcac aatatcttca aaaagttatg acggaatgat aaatgcaaga
     1201 ttcgttccat cgcagtaggc ctttcctcac aggaaaaagg attggcgcct tgcctctcat
     1261 accggaaaat taaataaaca atgttaattt ttagagaaaa ggaacgattc aatggagggg
     1321 aaaagaaaga acatgctact aagataaatg gattacttgt acgagcaaat tgtcgaacat
     1381 acgagctaag ggattatggt gactgatgcc gacgcccgca ttcgttcaaa cggttcaacg
     1441 aacgtacggc catcaatgcg gagacgactg tttgcgcaaa gtccgccttg tcgctgaagc
     1501 aaacagccaa gcaatacggc gggacggcag cgcgctatgg ggggaggaat ttgccgttat
     1561 tttgccgcac acttcgccgt ccgaagcagc ggctgctgct gaggccatac gggcaaacgt
     1621 cgagcggctc gccattccgc acggttcctc gcccgctgct tgtgtcgtta cagtcagcgt
     1681 cggagccgcc gtcgccgtcc cgcaaccaaa tgagagcgcg gaaaccgttc tttcccttgc
     1741 cgatcaggcg ctttatcgag ccaaacaaaa cgggcgcaac cggatggaaa tcgcctgtcc
     1801 cgctcgtgga tgaacctcct ccttttctca aaagaagcga gatatcgtca agcgttcagt
     1861 gcagatacgc aacaatcaga aaagggtgcc ccgcttcttt cgggacaccc tcatgttcat
     1921 gtactgacgt tatcggttac atcgtccgtt gccggtcagg gaacaacagg ctaaacagca
     1981 ccccggccgc cagcgcaatg gcaaatgtcg taaaccgcga gctaacgatg gcctcaagcg
     2041 gagaagaaat ccaaatgacc gtgctgacaa aaccggccac aatcgtttcg atgacgccga
     2101 cgccggaata ccgcttccag aagaaagaga gcaaaatcgc cggcgacaac gtgcagccga
     2161 tccctgccca tgcccaactg acaatgaaat acacaagcga ctccgatgtc atcgccagca
     2221 ccaagccaag cagcccggcg atgaccacta ccgcgcggga aagcgcgaca agctgttttt
     2281 ccgacaggcg aaggcgcagc gagcggcgga caatatcttc gctgatggag ctcgtgaatc
     2341 acgagcagct gcgaatcggc cgttgtcacg atgcggccaa aatgccggac aaaagcactc
     2401 ccgcgagcca cggcggcagc aagtcgagcg ccatgtgcgg caacaccgtt atgtgtcaac
     2461 ccatcctgtt attccaacat gaggcgcgtc ctacatcgga atgtttcgct ctcgtccgga
     2521 cagcgtccac atgtaaagtt ccctaagttg caaggcggtg ggaattataa taaagtggag
     2581 atgaatccaa aaaaaggagt aacaagatga atcgctggga aaaagaattg gacaagtacg
     2641 ccgaactggc ggtgaaagtc ggggtgaaca ttcagcccgg gcagacgctg ttcgtcaacg
     2701 ccccgctcga agcggcgccg ctcgtccgca aaatcgccaa aaccgcgtac gaaaccgggg
     2761 cgaaacacgt gtatttcgag tggaacgacg aggcgcttac atacattaaa tttcaccacg
     2821 ccccagaaga agcgttttcc gaatatccga tgttgcgggc aagagcgatg gaagaactcg
     2881 ccgaacaagg cgctgccttt ttatccattc acgccccgaa tccggacttg ttaaaagacg
     2941 tcgatccaaa gcggatcgcg accgccaata aaacagcagc gcaagcgctc gccaactacc
     3001 ggagcgccat tatggccgac cggaactgct ggtcgctcat ttccgtcccc acaccggcgt
     3061 gggcgcaaaa agtatttggc gatctgcgcg atgaagaagc catcgacaaa ttatgggaag
     3121 cgattttccg catcacccgc atcgaccaag acgatccgat cgctgcctgg cgcgaacata
     3181 acgaccggct cgcccgcatc gtcgattact taaacaacaa gcaatacaaa cagctcgttt
     3241 acgaagcgcc aggaccaatc ttcacagtcg agctcgtcga cgggcacgtc tggcacggcg
     3301 gcgcggcgac gagtcaaagc ggcgtgcgct tcaatcccaa catcccgacc gaagaagtgt
     3361 ttaccatgcc gcataaagac ggcgtcaacg gcacagtgcg caacacgaag ccgctcaatt
     3421 acaacggcaa cgtgatcgat ggttttacgc tcacgttcaa agacgggcaa gtcgtcgact
     3481 tcagcgccga acaaggatat gagacgctta agcacttgct tgacaccgat gacggcgcgc
     3541 gccgcctcgg ggaagtcgcc ctcgtgccgc accaatcgcc ggtgtcgctg tccaatctca
     3601 ttttttacaa cacgctgttt gatgaaaacg ccgcttgcca tttggcgctc ggcaaggcgt
     3661 atccgaccaa tatcgaaaac ggcgcctctc tttccaaaga ggagcttgac cgccgcggcg
     3721 tcaacgacag cctcgtccac gtcgacttta tgatcggctc agctgatctg aacattgacg
     3781 gcgtgacgaa agacgggaag cgcgagccga ttttccgcag tggcaactgg gcattcgaac
     3841 tggcgtaatc ggtttcgcca gctctaaaca attaagtgcg ccaaacgagc ggccagaccc
     3901 gaccgttgcg cagccttatt ccgttcaagc acggttttgc acggaaccat tagaccgaac
     3961 ggattgattt gttttcgtcc tgtagcaatt gcacagcggc agatgccgcc tgcgccagct
     4021 gctcgcattg ccgcaaacga atgtcaaaga gcacgagcgg gtcgcggtac gcctcagggt
     4081 ttttgcgcat atgtttgagc tcgccttcct gggcagcgat ctccagctcc agccgtttca
     4141 acgtttcatc gagcgccgtc aatggcgcat ggaaaaattg agtgacatca cgctcaagcg
     4201 atttggcaaa ccattcaaac ggcagcaaca actgctggat gatcgcctcc accacctcag
     4261 cgtaatcttg cgtctggttc aactgcttat acttttgcgc cagatcgcct tgttttgcgc
     4321 aaaagcaccg agcagtttgc ccgccccttt caacagcagc tggcttggcg ttctccgcaa
     4381 gaaaacgttc accttgccca cttgcacccc gttgatcaac cgatccgtat cgcgccgcca
     4441 atcggcgaga aggtgaaaat cgcattcgag cttgagtcgt tcctggccga acatggcgtt
     4501 aaacccttcg cccatttcat gcaaaaacac ctggcattcg ttgaattc
//