From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Plasmid P6 from Shigella sonnei 47 restriction endonuclease (SsoIR)
Date: 28 Jul 92 10:07:46 GMT
Distribution: bionet
Checksum: 44505     5

LOCUS       PP6SSOIRM    1993 bp ds-DNA             BCT       28-JUL-1992
DEFINITION  Plasmid P6 from Shigella sonnei 47 restriction endonuclease (SsoIR)
            gene complete cds, methyltransferase (SsoIM) gene, complete cds.
ACCESSION   M97479
KEYWORDS    methyltransferase; restriction endonuclease.
SOURCE      Plasmid P6 DNA.
  ORGANISM  Plasmid P6
            Prokaryotae.
REFERENCE   1  (bases 1 to 1993)
  AUTHORS   Karyagina,A.S., Lunin,V.G. and Nikolskaya,I.I.
  TITLE     Cloning and sequencing of methyltransferase and restriction
            endonuclease SsoI genes: strong homology to nucleotide sequence of
            the EcoRI genes
  JOURNAL   Unpublished (1992)
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     CDS             101..934
                     /function="cleavage of DNA (G^AATTC)"
                     /standard_name="R.SsoI"
                     /product="restriction endonuclease"
                     /gene="SsoIR"
                     /evidence=EXPERIMENTAL
                     /codon_start=1
                     /translation="MSNKKQSNRLTEQHKLSQGVIGIFGDYAKAHDLAVGEVSKLVKK
                     ALSNEYPQLSFRYRDSIKKTEINEALKKIDPDLGGTLFVSNSSIKPDGGIVEVKDDYG
                     EWRVVLVAEAKHQGKDIINIRNGLLVGKRGDQDLMAAGNAIERSHKNISEIANFMLSE
                     SHFPYVLFLEGSNFLTENISITRPDGRVVNLEYNSGILNRLDRLTAANYGMPINSNLC
                     INKFVNHKDKSIMLQAASIYTQGDGREWDSKIMFEIMFDISTTSLRVLGRDLFEQLTS
                     K"
                     /citation=[1]
     CDS             964..1944
                     /function="methylation of adenine in the sequence GAATTC"
                     /standard_name="M.SsoI"
                     /product="methyltransferase"
                     /gene="SsoIM"
                     /evidence=EXPERIMENTAL
                     /codon_start=1
                     /translation="MARNATNKLLHKAKKSKSDEFYTQYCDIENELQYYREHFSDKVV
                     YCNCDDPRVSNFFKYFAVNFDNLGLKKLIASCYVENKEGFSSSEAAKNGFYYEYHKEN
                     GKKLVFDDISVSSFCGDGDFRSSESIDLLKKSDIVVTNPPFSLFREYLDQLIKYDKKF
                     LIIANVNSITYKEVFNLIKENKIWLGVHLGRGVSGFIVPEHYELYGTEARIDSNGNRI
                     ISPNNCLWLTNLDVFIRHKDLPLTRKYFGNESSYPKYDNYDAINVNKTKDIPLDYNGV
                     MGVPITFLHKFNPEQFELIKFRKGVDEKDLSINGKCPYFRILIKNKRLQK"
                     /citation=[1]
BASE COUNT      696 a    258 c    393 g    646 t
ORIGIN
        1 cgtttcagcg gtgcgtacaa taagggatta tggtaaatca aacgtatgtt aatctatcga
       61 catatgtaac tttataaaat aacagtggaa acatggattc atgtctaata aaaaacagtc
      121 aaataggcta actgaacaac ataagttatc tcaaggtgta attgggattt ttggggatta
      181 tgcaaaagct catgatctcg ctgttggtga ggtttcaaaa ttagtaaaga aagctcttag
      241 caacgaatac cctcaattat catttcgata tagagatagt ataaagaaaa cagaaataaa
      301 tgaagcttta aaaaaaattg accctgatct tggcggtact ttatttgttt caaattccag
      361 catcaaacct gatggtggaa ttgtagaggt caaagatgat tatggtgaat ggagagttgt
      421 acttgttgct gaagccaaac accaaggtaa agatattata aatataagga atggtttgtt
      481 agttgggaaa agaggagatc aagatttaat ggctgctggt aatgctatcg aaagatctca
      541 taagaatata tcagagatag cgaattttat gctctctgag agccactttc cttacgtcct
      601 tttcttagag gggtctaact ttttaacaga aaatatctca ataacaagac cagatggaag
      661 ggttgttaat cttgagtata attctggtat attaaatagg ttagatcgac taactgcagc
      721 taattatgga atgcctataa atagtaatct atgtattaac aaatttgtaa atcataaaga
      781 caaaagcatt atgctacaag cagcatctat atatactcaa ggagatggga gggagtggga
      841 ttcgaaaatt atgtttgaaa taatgtttga tatatcaacg acttcgctca gagtgttggg
      901 gcgtgacttg tttgaacagc ttacatctaa gtgatatttt ttattttaat aaggttttaa
      961 ttaatggcta gaaatgcaac aaacaagtta ctgcacaaag ctaaaaaatc gaaaagcgac
     1021 gaattttaca ctcagtattg tgatattgag aacgaactgc aatactacag agagcacttc
     1081 tctgataagg ttgtttattg caattgtgat gatcctagag taagcaattt ctttaaatat
     1141 tttgcagtga attttgataa tcttggcttg aaaaagttaa tagcatcttg ctatgtagag
     1201 aataaagaag gtttttctag tagcgaagcc gcgaagaacg gattttacta tgaatatcat
     1261 aaagaaaatg gaaagaaatt agtttttgat gatattagtg tttcttcttt ctgtggcgat
     1321 ggcgattttc gcagttcgga gagcattgat ctgctaaaaa aatcagatat tgttgttacg
     1381 aatcctccat tctcgttatt tagagagtat cttgatcaac taattaagta tgataagaaa
     1441 ttccttataa ttgcaaatgt taattcaata acatataaag aggtgtttaa tctaattaag
     1501 gaaaataaga tttggcttgg ggttcatctc gggagaggtg tttctggatt tattgttcca
     1561 gagcattatg aattatatgg tactgaggcg agaattgatt ctaatggtaa tagaattatc
     1621 tcgccaaaca actgcttatg gctaactaac ctagatgtct ttattaggca taaagacttg
     1681 cctcttacaa gaaaatattt tgggaatgaa agttcatatc caaaatatga taattatgat
     1741 gctataaatg taaacaaaac aaaggatatt ccattagatt acaatggggt tatgggggtt
     1801 cctatcacat tcttgcataa gtttaaccct gagcaatttg agttaataaa atttagaaag
     1861 ggtgttgatg aaaaagattt gtctataaat ggtaaatgcc cttatttcag aattttgata
     1921 aaaaacaaac gattacaaaa gtaattgatg tttgttagtt ttttcttgag atcattagct
     1981 tcgtcgtaag ctt
//