From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Saccarum hybrid phosphoenolpyruvate carboxylase (SCPEPCD1) gene,
Date: 29 Dec 92 00:47:17 GMT
Distribution: bionet
Checksum: 39845 16

LOCUS       SCFSCPEPCD   8638 bp ds-DNA             PLN       03-DEC-1992
DEFINITION  Saccarum hybrid phosphoenolpyruvate carboxylase (SCPEPCD1) gene,
            complete cds.
ACCESSION   M86661
KEYWORDS    phophoenolpyruvate carboxylase.
SOURCE      Saccharum hybrid (variety H32-8560) (library: lambda EMBL4) DNA.
  ORGANISM  Saccharum hybrid
            Eukaryota; Plantae; Embryobionta; Magnoliophyta; Liliopsida;
            Commelinidae; Cyperales; Poaceae.
REFERENCE   1  (bases 1 to 8638)
  AUTHORS   Henrik,A.H., Martin,T. and Sun,S.S.
  TITLE     Structure and expression of a sugarcane gene encoding a
            housekeeping phosphoenolpyruvate carboxylase
  JOURNAL   Plant Mol. Biol. 20, 663-671 (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     exon            1766..1933
                     /number=1
                     /gene="SCPEPCD1"
     intron          1934..2729
                     /number=1
                     /gene="SCPEPCD1"
     exon            2730..3122
                     /number=2
                     /gene="SCPEPCD1"
     intron          3123..3462
                     /number=2
                     /gene="SCPEPCD1"
     exon            3463..3546
                     /number=3
                     /gene="SCPEPCD1"
     intron          3547..3682
                     /number=3
                     /gene="SCPEPCD1"
     exon            3683..3904
                     /number=4
                     /gene="SCPEPCD1"
     intron          3905..4022
                     /number=4
                     /gene="SCPEPCD1"
     exon            4023..4127
                     /number=5
                     /gene="SCPEPCD1"
     intron          4128..4203
                     /number=5
                     /gene="SCPEPCD1"
     exon            4204..4293
                     /number=6
                     /gene="SCPEPCD1"
     intron          4294..5457
                     /number=6
                     /gene="SCPEPCD1"
     exon            5458..5613
                     /number=7
                     /gene="SCPEPCD1"
     intron          5614..5836
                     /number=7
                     /gene="SCPEPCD1"
     exon            5837..6835
                     /number=8
                     /gene="SCPEPCD1"
     intron          6836..7145
                     /number=8
                     /gene="SCPEPCD1"
     exon            7146..7532
                     /number=9
                     /gene="SCPEPCD1"
     intron          7533..7816
                     /number=9
                     /gene="SCPEPCD1"
     exon            7817..8113
                     /number=10
                     /gene="SCPEPCD1"
     polyA_signal    8371..8376
                     /gene="SCPEPCD1"
     CDS             join(1766..1933,2730..3122,3463..3546,3683..3904,
                     4023..4127,4204..4293,5458..5613,5837..6835,7146..7532,
                     7817..8113)
                     /EC_number="4.1.1.3.1"
                     /gene="SCPEPCD1"
                     /product="phosphoenolpyruvate carboxylase"
                     /codon_start=1
                     /translation="MARNAVDKATSIDAQLRLLAPQKLSDDDKLVEYDALLLDRFLDI
                     LQDLHGEDIRETVQECYELAAEYENKLDPKMLDEIGNVLTSLDPGDSIVITKSFSHML
                     ILANLAEEVQIAYRRRIKLKKGDFVDENSATTESDIEETLKRLMHQLKKSPLEVFDAL
                     KNQTVDLVLTAHPTQSVRRSLLQKHGRIRNCLTQLYAKDITPDEKQELDEALQREIQA
                     AFRTDEIRRAPPTPQDEMRAGMSYFHETIWKGVPKFLRRVDTALKNIGINERLPYNAP
                     IIQFSSWMGGDRDGNPRVTPEITRDVCLLARMMAANLYNAQIEDLMFELSMWRCSDEL
                     RVKVDELHRSSKKDTTKHYIEFWKQVPPSEPYRVILSDVRDKLYNTRERARHLLASGF
                     SEIPEEATFTDVEQFLEPLELCYRSLCACGDRSVADGSLLDFLRQVSTFGLSLVRLDI
                     RQESDRHTDVMDAITEYLGIGSYRKWTEEKRQEWLLSELNGKRPLFGPDLPKSDEIAD
                     VLDTFHVLAELPSDSFGAYVISMATAPSDVLAVELLQRECHVKKPLRVVPLFEKLADL
                     EAAPAALARLFSVEWYRNRINGKQEVMIGYSDSGKDAGRFSAAWQLYKAQEELINVAK
                     LYGVKLTMFHGRGGTVGRGGGPTHLAILSQPPETIHGSLRVTVQGEVIEQSFGEEHLC
                     FRTLQRFTAATLEHGMHPPISPKPEWRALMDEMAIVATKEYRSIVFEEPRFVEYFRLA
                     TPEMEYGRMNIGSRPSKRKPSAGIESLRAIPWIFAWTQTRFHLPVWLGFGAAFKHVLD
                     KDIRNLQTLQEMYNQWPFFRVTIDLVEMVFAKGDPGIAALYDKLLVSEDLWSFGKRLR
                     ANYEETKQLLLQVAGHKDLLEGDPYLKQRLRIRDSYITALNVCQAYMLKRIRDPGFQV
                     NPGPHLSKDIMDMGKPASELVKLNTTSEYAPGLEDTLILTMKGIAAGMQNTG"
BASE COUNT     2272 a   1794 c   1863 g   2707 t      2 others
ORIGIN      
        1 gatccgtcac tgtcctcatg taatactcac accctttgga tacaaaaata cgagcatacg
       61 gctatgtttt caagagtaca acaacttctt tgccatgtct accatagctt gcccaatata
      121 aaaatcgaac atatctctct agagccaacc tccttcattc attattcatg tccatggaca
      181 aaccacaaaa ttcactcgat tttcactcta gatggtgtga taaatcttta tctatctatc
      241 tgtctgtcta ggtagccagc tggttttttt ttatctgtct gtctatctat ctatctatta
      301 ttaaagaata aaactttact tccaactcat tttgatttat tcccaaaaaa tacatgtcct
      361 ttctttcgtc ccattctttt tttaaaatgt gttgaattta atgcaacata cggacacact
      421 tgctgacgaa caaaattccg ataaaacaaa ggtcttgctc tcccgacgcg acgggccgcc
      481 accatgaaac ctgtaggcga gagccgagag gagtaagact atccgcactc gtggtactct
      541 aaattcatca tctaaaagca atatttcatt atagtcactc ctctatacca ctttctttcg
      601 cactcatcca tcctctgtaa tcatcctcta taccaactac cagctgatgg ggtccacacg
      661 tcatactctt attttctctc tcccttcccc tctcctccct cttctctctc ctctatcttc
      721 ttttccggct gcttccggtt gggcgcagca cacgaggtgg ggccgccctg ggcacgtagc
      781 ggactgctgc gacatgccag gaatggcgga cgatgagtgg gtagcggtcg gtttgcgtcc
      841 ccggtgcagc cgatttgagt cgctatgtcc acgctgtccc tttagcgctc gtgtatacgt
      901 cccggtgcgg acggtcttaa cgctccacta ggatgggctt tcaccactgg agatgaaaac
      961 gaaacagaaa tattccgaat tgactgtttt cgttttctat attatgaacc ggttttttaa
     1021 catgaaaaat tgtcagtttc aattggtttc acaatcattt tctaccattt ttttgtattc
     1081 tttaattgct aaatgtagaa atattccgaa ccgaccgttt ttatttttct atactgtaag
     1141 ctcgttttcg accgttttgt tcctggttca ccgccacagt ccgagttcga agggatatac
     1201 gacgaatcca tccagcacaa gcgacagtcc cgtcctgtcc tataaatagc agaccacagc
     1261 agagagagag agagggtgtc cctctccgct ccgtcacagg aaacccggta tagcatcaag
     1321 gaangttttt ttgtcgtgtt gattgatctc cctccccttt cgcccccgtc ctgcctcctg
     1381 gcctcacccc agaaaaaaaa aggtgagatg ctttcctctc ctctcccttc gatccagtag
     1441 gtttgcagtc tgtttcctag atcttgttta ggcatttgtc catcttggag tggcaaagtc
     1501 cctagctccc acaacggctg attgctgtag gttttgatca tacttttttt tttgcattcg
     1561 agaaagcccc gtgctttgtt ggaatctgcg tgcttagggc tgggtggatc gtcttctcgc
     1621 ttcaagaaca tgtcgaaatc cgtggattgg agggatttgt ggggttttct cttctctttt
     1681 ggttggaatt gtgggttttg atctgtgtgg attctgttgc agggggcgag aagggaaggg
     1741 gagtgcttgg gggggagaga agtagatggc gcgcaatgcg gtggacaagg cgacgtccat
     1801 cgacgcgcag ctgcggctgc tggccccgca gaagctctcc gacgatgaca agcttgtcga
     1861 gtacgacgcc ctgctcctcg accggttcct cgacatccta caggacctgc acggcgagga
     1921 catcagggag acggtatgta aatgctccaa ttgtttcctg cccgtggtgc tatttgttcc
     1981 tttcttttcg gtgctggcaa ttgatttagg tggtagatag ctcgatcatg cttgaaaatc
     2041 tgtttatact gtcatgcatg catgggtcgc gatagcagga gaatcagaca cagcatctgg
     2101 gccttgtccg tgggtgtttc tatatggtga tattcttctc atctgttttg gtattgcact
     2161 ctggtgatgg aaactagtgc tgcatttaac aaatcatagt agcactgggg gtccacccat
     2221 ttgttctaca gttgctgatc aatactgtgc tagttcccac tttgtgtttt ctagcttttg
     2281 aaattggtac atcaccatga tgttcaaaac tggtggggtt atcatgatgt gtggagcact
     2341 tgcactataa tcatggcctt ttgatactct cgagttatta tgccctttga actattgttg
     2401 ttgctgatac gaggtagttt gcagatcaga tttattgtct tttctatgca taaatagtat
     2461 gataaaatgg taaaaggcca ccttttttac aatgcataga cctatcacgc tattttacat
     2521 tatcatttta ccactcaagt aagtcccatc tccttgccca ttttttttgt atatctgcaa
     2581 gccaatcttt gcttgacatt ctgataggtg gcttgatttc tgtggtcatc cgttacacag
     2641 caattccagt gtgttcacca actcactcta cattcgataa aatttatact tttttgcaat
     2701 gccgtaaagt ttccctttct catgaatagg tgcaagaatg ctatgagtta gctgctgagt
     2761 atgaaaataa gcttgacccc aaaatgctgg atgagattgg gaatgtgctg accagcttgg
     2821 atcctggaga ctccattgtc ataaccaagt cattctcaca catgcttatc ctggcaaact
     2881 tggctgagga ggtacaaatt gcataccgca ggagaataaa actgaagaag ggtgactttg
     2941 ttgatgagaa ctcagcaaca actgaatcag acatcgagga gacattaaag aggcttatgc
     3001 atcagcttaa gaagtcccct ctggaggtat ttgatgctct caagaatcaa actgttgacc
     3061 tcgtcctgac tgcacatcca actcagtcag tccggagatc actgctccaa aagcatggca
     3121 ggttagtctt gaagctttct gaattgttag atctcaggat gagatttact cgatggaatt
     3181 gaatatttgc tatgcagaaa tgttggttct gtttgttctt tatgcacatc agagcaatgc
     3241 aatttgctca cactgaattt cacatactgc tttcatagaa aacctgatgt ttttttgtcc
     3301 acttttcttt tatgtgtaaa tattccttgc atggttttgc tgtagtctgc cttttaagaa
     3361 acaacagcaa tagaaaaaaa taatatattt ctcgaggcat gttaaatcat acgttatatg
     3421 tgccttttga tgccttagat tcctcacctt tcaatgtgca ggataagaaa ctgtttaact
     3481 caactttatg caaaggacat cactccagat gagaaacagg aacttgatga agcacttcaa
     3541 agagaggtaa tttgcttaga tattttggta tattagtgtt gtataatact atctgtgtag
     3601 tagttaatca ggaaaacttt gtcaccttca ctaaatatat atttatatga ttctaatatc
     3661 ctgttccatt tcttatctac agattcaagc tgcctttaga acagatgaaa tcaggcgggc
     3721 gcctcctact ccgcaggatg aaatgcgtgc tggaatgagt tattttcatg agactatatg
     3781 gaagggtgta cccaagttct tacgtagggt tgacactgct cttaagaaca ttggcataaa
     3841 tgagcggttg ccatataatg cccctatcat tcagttttct tcttggatgg gtggtgatcg
     3901 tgatggtgca tggcattctc tctccttttt tttggatagt atatttgatg atgtgccgaa
     3961 tcatgcaact gtagttcaca agaaagaagt gcatatataa ctctaaactg atatccacaa
     4021 cagggaatcc aagagtcacg ccagagatta caagggatgt atgcttgttg gctagaatga
     4081 tggctgctaa tttgtataat gcccagatag aagacctaat gtttgaggta aaaataagga
     4141 cacagttttt atttgatatt gtagtgatat tagtctcaca agaattcttt ttatcgcttg
     4201 cagttatcta tgtggcgctg cagtgacgag ttacgtgtaa aggtcgatga gttacaccga
     4261 tcctccaaga aagatacaac aaaacactat ataggtgcag attatattat gatatttttg
     4321 ttaaacttat atattatatc ttcaacatcc ttgcctacca tctgctgcaa aatatttaac
     4381 caaactagtc gccaatctgt tctttccaat ggatgaaata gctacacatt ttataaagta
     4441 cctccatcca gaaaagagtg cagttctagt tctactctta gtcaaacaat ctaaagtttg
     4501 accaagttca tagaaaagaa tatcaataat tatgacacca aataggtata ctatgaaaat
     4561 atatttcatg acaaatctaa tgatacttat ttggtatcat aaatattgat atttttttgg
     4621 cacaaagtta gtcaaacttg agatggtttg acttagtcat aaatatattt ggtatccaga
     4681 attgcattct tttccggatg aagggagtat cttacttatg attaatatat ggatcctcat
     4741 cataaccata aatgtggttc cactatgctg ctgaagatta atctgagaca tgcagatcag
     4801 gattctaatc taaatggttc taacccaagt cagcaattgg ggaaaaaata ggcatggcaa
     4861 atccatattc caaaagggtt ggactgtaag aatcagaaac agttaaaagg gcagacccag
     4921 tgccagaggc tcccacacga gtggggtcta gggaaaggaa taaccgaagt aaagtcttac
     4981 ctccgcagaa attctacaga gagaaacagt taccttgcaa aaaaaaaggc attgaaggat
     5041 ttgaaacatg caataaaact aatgtattta gctgttagct taataaatat gatcatgaag
     5101 tattaagaaa tagctgtcat caggactcag gagttgcatt gatatatgtg ctttcttgcc
     5161 aacataggct ggmatttgaa tcatgtttcg ttctcttgat ttgtttctga gacacgtgga
     5221 tgtctatttc tgtcgttgta ctattttaat attggaaaga accatgtcac tatggagtaa
     5281 tttttttaaa gacaaattct tgctattttc atgcagactt tgatatttag gatgacattt
     5341 ttggcagcaa cagacatgat attgatgcat gctatttttg tctattcttt tagattaatg
     5401 cagccaatta gacaaaacta ggtgaagtta atacatgctg acatgctttt ccatgcagag
     5461 ttctggaaac aagttccccc cagtgaacct taccgtgtga ttctgagtga tgtcagagat
     5521 aaactctaca acacgcgtga acgagcacgc catttattag ctagtgggtt ttctgaaatt
     5581 ccagaggaag caaccttcac cgatgttgaa caggtatgta aagtgaaaac agtagtgttc
     5641 atttgatgtt cctggctagg ataaaaaatt aatctgtacc atacactttt agtcctatat
     5701 taagattgga ttctttcatt ctatggtgta ctgtaaaagg ttgaaacatt cccaagataa
     5761 aggatctttg tagctgtttc aggatatatt atgttttatg aattcttgtc tcgattgaat
     5821 cttttttctc ttgcagttct tggagcctct tgagctctgt tacagatccc tctgtgcctg
     5881 tggtgatcgc tctgttgcag atggaagcct tcttgacttc ttacggcaag tgtctacgtt
     5941 tggactatcc cttgttagac ttgatatcag gcaagaatct gaccgacata ctgatgttat
     6001 ggatgctata actgaatacc tcggcattgg atcataccgt aaatggacag aggagaaacg
     6061 ccaagaatgg ctactctcgg aacttaatgg aaagaggccg ttatttggtc ctgaccttcc
     6121 caagtcagat gagattgctg atgttctaga tactttccac gtattagctg aacttccttc
     6181 agacagcttt ggtgcatatg taatatctat ggcgacagct ccctcagatg ttcttgctgt
     6241 cgagctcttg cagcgtgaat gtcatgtgaa aaaaccactg agagttgtgc cattgttcga
     6301 aaaactggca gatcttgagg cagcacccgc agctttagct cggctgttct ctgttgaatg
     6361 gtacagaaac aggatcaatg gcaagcagga agtgatgatc gggtattcag attctggcaa
     6421 ggatgctggt cgtttctctg ctgcttggca actgtacaag gctcaagagg agctaattaa
     6481 tgttgctaag ctgtatgggg ttaagttaac tatgttccac ggacgaggtg ggactgtggg
     6541 aagaggtggg ggccctaccc atcttgctat actgtcacaa cctcctgaaa ctattcatgg
     6601 atcacttagg gtaactgttc aaggtgaagt cattgagcaa tcttttggag aggagcattt
     6661 gtgctttaga accctgcaac gtttcacagc tgctactctt gaacatggta tgcatcctcc
     6721 aatttcgcca aaaccggaat ggcgtgcttt aatggatgaa atggctattg ttgccacaaa
     6781 ggaataccga tcaattgtat ttgaagaacc acgctttgtt gaatatttcc gccttgtaag
     6841 tcttcggttt tctttgcttg tgttttcctt ttgaacacac ctttacttgt ctttatattg
     6901 ttattatgca attaccacta tgagatttta attgtagctt ttatttaggc cttggtattt
     6961 cactcttgtt tttgttagtt gggaattcat ctagctagtc ctgaagatta atatactgtt
     7021 tcatttcgtt tgttttactg tcatgtcatt actccttgta gtaccctata aatttattat
     7081 tttttggtaa tgggtccatt tttctgcata actaatcctg gcatttgtgt ctttctttca
     7141 aataggcaac accagagatg gaatatggta ggatgaatat tggaagcaga ccatcgaaaa
     7201 gaaagccaag tgcaggcatt gaatcactgc gtgcaatccc ttggatcttt gcttggacgc
     7261 agactcggtt ccacctacca gtgtggcttg gttttggagc ggcattcaag catgtcttgg
     7321 ataaggacat acggaatctt caaacccttc aagagatgta caatcagtgg ccatttttca
     7381 gggttacaat agacttggtt gagatggtgt ttgccaaagg cgatcctggt atagcggctc
     7441 tgtatgacaa gcttctagtt tcagaggatt tgtggtcatt cggcaagcgt cttagagcca
     7501 actatgaaga aacaaagcaa cttcttctac aggtgatttg tctaagatct tcttgcattc
     7561 gttatggact gctagagttg catgtagact atgtgatgcc tctaccaggc ttgaacattg
     7621 gtgcgtctga atgagttgat aattaagtta atccgtaatc tatagccaca tcatgcccaa
     7681 ctatttccag agaatgttta gatacaagct caaactggta gataaaccaa ggaggaaatg
     7741 gttctggcaa taggatgtcc attcaccccg tcttgttctt gtctcaccca ctgcattgtg
     7801 gcctgctgaa tttcaggttg ctgggcacaa agaccttctg gagggcgacc cttacctgaa
     7861 gcagaggctg cgcatccgtg actcctacat cactgccctg aacgtgtgcc aagcttacat
     7921 gctgaagcgc atcagggacc ctggcttcca ggtgaacccg gggcctcacc tgtcgaagga
     7981 catcatggac atgggcaagc cggcctcgga gcttgtgaag ctcaacacca cgagcgagta
     8041 cgccccggga ctggaggaca ccctcatcct gaccatgaag ggcatcgctg ccggaatgca
     8101 gaacaccggc tgaaagaaca ggatccggag tgccttcttt attctgagct gatgtatcta
     8161 tcgtagcgta ggggatcatg gcgtgaaccg gttaacaaga cgggggtgca cacctggata
     8221 tgggggatta cctgttttag cagtagtagt atttactttt atgctatgct ctatcgaaac
     8281 tcctatgtat gcatatccat gttgcttgag cctgcactag cttttggatc atagcttgtt
     8341 aagtaatgcg cagggcatct tgttacggtg aaataactct gtgcttgtgc ttggtgctgg
     8401 gctgctggca tatttgtgcc gaggagtcta ctgaattgca tggatcattg taccttttct
     8461 gtcgtgcggt atcttgtcga atactactgt cctgggctaa cggacaggct gtagagacta
     8521 gatgcgcacg tcgtgtatca cgcacgcaca acggccccct ggaaactgcg gaggcccgga
     8581 gccctcggac gtgcacgcgc aaacaggggc tttgtcaaaa ttcaattttt cttctaat
//