From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Synthetic plasmid pDSP1: a cloning vector for eukaryotic genes and
Date: 24 Jul 92 10:10:15 GMT
Distribution: bionet
Checksum: 58194     5

LOCUS       SYNDSP2      2143 bp ds-DNA             SYN       24-JUL-1992
DEFINITION  Synthetic plasmid pDSP1: a cloning vector for eukaryotic genes and
            gene regulatory elements, segment 2.
ACCESSION   K03394
KEYWORDS    xgprt gene.
SEGMENT     2 of 2
SOURCE      SV40 DNA and E.coli DNA.
  ORGANISM  Cloning vector
            Artificial sequences; Cloning vehicles.
REFERENCE   1  (bases 1 to 2143)
  AUTHORS   Reff,M.E.
  JOURNAL   Unpublished (1986) Smith Kline and French Labs, Swedeland PA 19479
  STANDARD  full staff_review
COMMENT     Draft entry and sequence in computer readable form for [1] and [2]
            kindly provided by M.E.Reff, 29-MAY-1986.
            Vector pDSP1 contains two independent mammalian transcription
            cassettes containing SV40 early gene regulatory elements
            controlling either the E.coli galK or xgprt genes.  The regulatory
            elements of the galK cassette are bounded by multiple restriction
            sites allowing for easy removal and replacement of the regulatory
            sequences or the galK gene itself.
FEATURES             Location/Qualifiers
     misc_recomb     <1..1
                     /note="pBR322 DNA end/SV40 early DNA start"
     misc_recomb     355..356
                     /note="SV40 early DNA end/xgprt gene start"
     CDS             434..892
                     /note="xpgrt"
                     /codon_start=1
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     misc_recomb     1312..1313
                     /note="xgprt gene end/SV40 DNA start"
     misc_recomb     1904..1905
                     /note="SV40 DNA end/SV40 poly-A early DNA start"
BASE COUNT      576 a    495 c    473 g    599 t
ORIGIN      About 1.6 kb after segment 1.
        1 gatccggctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg
       61 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg
      121 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc
      181 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
      241 tgcatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
      301 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctgatct
      361 ctataatctc gcgcaaccta ttttcccctc gaacactttt taagccgtag ataaacaggc
      421 tgggacactt cacatgagcg aaaaatacat cgtcacctgg gacatgttgc agatccatgc
      481 acgtaaactc gcaagccgac tgatgccttc tgaacaatgg aaaggcatta ttgccgtaag
      541 ccgtggcggt ctggtaccgg gtgcgttact ggcgcgtgaa ctgggtattc gtcatgtcga
      601 taccgtttgt atttccagct acgatcacga caaccagcgc gagcttaaag tgctgaaacg
      661 cgcagaaggc gatggcgaag gcttcatcgt tattgatgac ctggtggata ccggtggtac
      721 tgcggttgcg attcgtgaaa tgtatccaaa agcgcacttt gtcaccatct tcgcaaaacc
      781 ggctggtcgt ccgctggttg atgactatgt tgttgatatc ccgcaagata cctggattga
      841 acagccgtgg gatatgggcg tcgtattcgt cccgccaatc tccggtcgct aatcttttca
      901 acgcctggca ctgccgggcg ttgttctttt taacttcagg cgggttacaa tagtttctag
      961 taagtattct ggaggctgca tccatgacac aggcaaacct gagcgaaacc ctgttcaaac
     1021 cccgctttaa acatcctgaa acctcgacgc tagtccgccg ctttaatcac ggcgcacaac
     1081 cgcctgtgca gtcggccctt gatggtaaaa ccatccctca ctggtatcgc atgattaacc
     1141 gtctgatgtg gatctggcgc ggcattgacc cacgcgaaat cctcgacgtc caggcacgta
     1201 ttgtgatgag cgatgccgaa gctaccgacg atgatttata cgatacggtg attggctacc
     1261 gtggcggcaa ctggatttat gagtgggccc cggatctttg tgaaggaacc ttacttctgt
     1321 ggtgtgacat aattggacaa actacctaca gagatttaaa gctctaaggt aaatataaaa
     1381 tttttaagtg tataatgtgt taaactactg attctaattg tttgtgtatt ttagattcca
     1441 acctatggaa ctgatgaatg ggagcagtgg tggaatgcct ttaatgagga aaacctgttt
     1501 tgctcagaag aaatgccatc tagtgatgat gaggctactg ctgactctca acattctact
     1561 cctccaaaaa agaagagaaa ggtagaagac cccaaggact ttccttcaga attgctaagt
     1621 tttttgagtc atgctgtgtt tagtaataga actcttgctt gctttgctat ttacaccaca
     1681 aaggaaaaag ctgcactgct atacaagaaa attatggaaa aatattctgt aacctttata
     1741 agtaggcata acagttataa tcataacata ctgttttttc ttactccaca caggcataga
     1801 gtgtctgcta ttaataacta tgctcaaaaa ttgtgtacct ttagcttttt aatttgtaaa
     1861 ggggttaata aggaatattt gatgtatagt gccttgacta gagatcataa tcagccatac
     1921 cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa
     1981 acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa
     2041 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     2101 tggtttgtcc aaactcatca atgtatctta tcatgtctgg atc
//