From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human chromosome 4 sequence-tagged site STS4-386
Date: 21 Aug 92 10:09:43 GMT
Distribution: bionet
Checksum: 36247     4

LOCUS       HUM4STS386    896 bp ds-DNA             PRI       21-AUG-1992
DEFINITION  Human chromosome 4 sequence-tagged site STS4-386
ACCESSION   L00831
KEYWORDS    STS; human chromosome 4; sequence tagged site.
SOURCE      Homo sapiens, clone p4-309 from a chromosome 4 plasmid library;
            vector pBluescript II KS + (Stratagene)
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 896)
  AUTHORS   Goold,R.D., diSibio,G., Dugaiczyk,A., Smith,K.A., Xu,H.,
            Magrane,G., Lang,D.B., Cox,D.R., Masters,S.B. and Myers,R.M.
  TITLE     Sequence-Tagged Sites for Human Chromosome 4
  JOURNAL   Unpublished (1992) see COMMENT for author address
  STANDARD  full automatic
COMMENT     PCR components: 25 ng of human genomic DNA, 10 pmol of each
            oligonucleotide, 200 micro-M dNTPs, 0.25 U Taq  polymerase (Cetus)
            in 10 micro-l of 50 mM KCl-20 mM  Tris-HCl, pH 8.3 (at room temp),
            2.5 mM MgCl-2.  Initial denaturation at 94degC for 1.5 min, then 30
            cycles of  94degC for 15 sec, 62degC for 23 sec, and 72degC for 30
            sec, followed by a final extension at 72degC for 3.5 min, using a
            Perkin-Elmer 9600 thermocycler. PCR-amplified product size 423 bp.
            STS sequence contains  simple sequence tandem repeats (CA and CT).
            Sequence submitted by:
            Human Genome Mapping Center
            Box 0925
            University of California San Francisco
            San Francisco, CA 94143-0925  USA
            Phone: (415) 502-1612   Fax: (415) 476-8391
            e-mail: hgmcprobes@cgl.ucsf.edu
FEATURES             Location/Qualifiers
     misc_feature    248..271
                     /label=Primer1
     misc_feature    complement(646..670)
                     /label=Primer2
BASE COUNT      245 a    215 c    173 g    253 t     10 others
ORIGIN
        1 gatctaatca ccccatatca gtcccctctc caacatgggg gattacaact ggacatgtga
       61 tttgggtggg gacacaaatc caaaccatat cagggaggca tcagggctca gagagaggat
      121 acatcaaaag ttagaagnga gtaagattta tctacaatgt ccttcacaaa gttgaagggc
      181 tgtatttgcc aggctatttc agttttgaca gaattaccac ctggatttga ttaaagcaaa
      241 gtaaaatcta ctgactccta caaatgggaa gccaggggac caagttgcct tttgtctaag
      301 agttgattca gggctcaagt gaggctgttg gtttnctttt ctctggnttg gtctctctct
      361 ctctctctct ctctctctct ctctctctca cacacacaca cacacacaca cacacacaca
      421 ccggncacan acacacacac acacattccc tacnttncca nccaactttc catgcttnaa
      481 ggagaagaca gcaccagtgc cctgagcttc acatccttcc tgttctaagg tcaagatgat
      541 aaaaggggtg gggaggaaat gtactgggct tccaagtcca acaactaaaa cgaggtcctg
      601 gtaactccat atgaaagaga ttttagtcat ttatggagta tccaagtgac cgtctgtatt
      661 ctatcatgat atggacttct gttctcctag tctggccctg aaacaccagg aaatgaaaat
      721 agttcattcc cattgggtac ttaccaaatg tcagcctctg ttcttttatt tatgtattat
      781 ctcactaagt tctttaagta actacttact gtagttctga tgtggtttgg ctgtgtcccc
      841 acccaagtct catcttgaat tgtaactcca caattcccat gtntcatggg aggagc
//