From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: D.pseudoobscura Adh gene for alcohol dehydrogenase
Date: 28 Dec 92 21:27:51 GMT
Distribution: bionet
Checksum: 50808 7

LOCUS       DROADHGN67   3457 bp ds-DNA             INV       01-DEC-1992
DEFINITION  D.pseudoobscura Adh gene for alcohol dehydrogenase
ACCESSION   X64476
KEYWORDS    ADH gene; alcohol dehydrogenase.
SOURCE      Drosophila pseudoobscura DNA.
  ORGANISM  Drosophila pseudoobscura
            Eukaryota; Animalia; Metazoa; Arthropoda; Uniramia; Insecta;
            Pterygota; Neoptera; Holometabola; Diptera; Brachycera;
            Cyclorrhapha; Schizophora; Drosophiloidea; Drosophilidae.
REFERENCE   1  (bases 1 to 3457)
  AUTHORS   Schaeffer,S.W. and Miller,E.L.
  TITLE     Estimates of gene flow in Drosophila pseudoobscura determined from
            nucleotide sequence analysis of the alcohol dehydrogenase region
  JOURNAL   Genetics 132, 471-480 (1992)
  STANDARD  full automatic
COMMENT     *source: strain=PS230; *source: isolate=Kiabab National Forest,
            Arizona; *source: chromosome=IV section 88;
               From EMBL    entry DPADHGN67;  dated 16-OCT-1992.
FEATURES             Location/Qualifiers
     mRNA            join(40..119,913..1018,1082..1486,1547..1962)
                     /note="adult mRNA (Adh)"
     mRNA            join(880..1018,1082..1486,1547..1962)
                     /note="larval mRNA (Adh)"
     promoter        8..14
                     /note="adult promoter (Adh)"
     prim_transcript 40..1962
                     /note="adult primary transcript (Adh)"
     exon            40..119
                     /note="adult exon 1a"
     intron          120..912
                     /note="adult intron"
     prim_transcript 880..1962
                     /note="larval primary transcript (Adh)"
     exon            880..1018
                     /note="larval exon 1"
     exon            913..1018
                     /note="adult exon 1b"
     intron          1019..1081
                     /number=1
     exon            1082..1486
                     /number=2
     intron          1487..1546
                     /number=2
     exon            1547..1962
                     /number=3
     polyA_signal    1939..1944
     polyA_site      1962
     CDS             join(926..1018,1082..1486,1547..1813)
                     /EC_number="1.1.1.1"
                     /gene="Adh"
                     /product="alcoholdehydrogenase"
                     /codon_start=1
                     /translation="MSLTNKNVVFVAGLGGIGLDTSRELVKRNLKNLVILDRIDNPAA
                     IAELKAINPKVTITFYPYDVTVPIAETTKLLKTIFAQVKTIDVLINGAGILDDHQIER
                     TIAVNYTGLVNTTTAILDFWDKRKGGPGGIICNIGSVTGFNAIYQVPVYSGSKAAVVN
                     FTSSLAKLAPITGVTAYTVNPGITKTTLVHKFNSWLDVEPRVAEKLLEHPTQTSQQCA
                     ENFVKAIELNKNGAIWKLDLGTLEPITWTQHWDSGI"
BASE COUNT      970 a    788 c    793 g    906 t
ORIGIN      
        1 cgtagtgtat ttaaggcact gcacatgtcg aggaccacga ttattgtcta cagtgcagtc
       61 agtagttgtc aggtgcagca ggcggaccag catttcttta aatctactca atcgatcaag
      121 taagtaactg taatccgagt acagtcgacc catcccactc ggttctgcag taactcaaga
      181 aatttcttga tagcgcagtt ccatttgtag aacgtcgttt aaaaaatcat ctcgttggag
      241 cttgggttgg aaaacgttcg agttgggcgt agaaaagttc aaatataaaa tatcaaaaaa
      301 gagaaaatat cttcccaaag cagctgtcca ttttttgtta aagtgataga tcaaaaattc
      361 gtatgacgta tttcccaagt gctctttgtg caaataattt cctcaattct aattctacaa
      421 gaagcacttt tggctcttgt ccagaaaaaa caatttgttt acacttttcg gttgataaca
      481 gaagcggacc gacggctaag taacataaca gtcgcctgtt aagtaatgcc ctcactctct
      541 gttagatggc cgcgcgtgtc gactccgata gaccagcaga cacgcagtag ttttcctcaa
      601 gaacctgaac tctaaacata gacataattt acgatacctt gagactcaaa taaaaataca
      661 agtatgagtg gtattctatt cctgattaga ctcgcacaca cacatacgag tattaacata
      721 aatttgccgg caaataacca aaacaaaacg aacgcaaacg atccgaacac gtaatgcgag
      781 agataagaaa cagaaaagct cgacgtgaaa gaagcttctg gcggaagctc ttgccgaact
      841 ccctataaat acgagacaga aaaccagaat aaatataata acaaactgtt gaattgtccc
      901 atcgattccc agatcaaaag acagaatgtc tctcaccaac aagaacgtcg ttttcgtggc
      961 cggtctgggc ggcattggcc tggacaccag ccgggagttg gtcaagcgta atctgaaggt
     1021 aagagagaac gaattccatg gaatctatgg aatcctaaat ttaaaaattc atttatttta
     1081 gaacctggtc atcctggatc gcattgacaa tccggctgcc attgccgaac tgaaggcaat
     1141 caatcccaag gtgaccatca ccttctatcc ctacgatgtg actgtgccca tcgctgagac
     1201 caccaagctc ctgaagacca tctttgccca ggtgaagaca atcgatgtcc tgatcaacgg
     1261 tgctggcatc ctggacgatc atcagatcga gcgcaccatt gccgttaact acacgggcct
     1321 ggtcaacacc accacagcca ttctggactt ctgggacaag cgcaagggcg gcccaggcgg
     1381 catcatttgc aacattggct ccgtcaccgg tttcaatgcc atctaccagg tgcccgttta
     1441 ctccggctcc aaggcggcgg tggttaactt tacctcctcc ctggcggtaa gaaatcctac
     1501 aactatccta ctccccaaaa ctaaatattt ctttgaaatc ccatagaaac tggctcccat
     1561 tactggtgtc actgcttaca ctgtcaatcc tggcatcact aagaccactc tggtccacaa
     1621 attcaactcg tggctggatg tggagccccg tgtggcggag aagctgctcg agcatcccac
     1681 ccagacctct cagcagtgcg ccgagaactt tgtgaaggcc attgagctga acaagaacgg
     1741 tgccatctgg aagttggatc tgggcacctt ggagcccatc acatggaccc agcactggga
     1801 ctcgggcatc taaaccatcc cagagactct atgggacatg gcgttttagc ttttagtttc
     1861 tttttttcca ctcaattgtt acgtatatac atacacatat ggcaataagg ctgatttgaa
     1921 ccccgttttg aatatgataa tacaaaatta tatttgagaa atttcaacaa aatcgataag
     1981 agctatagct ctcttttaat taaaaaaaac tagataaaaa gagcaatggt cagtggtggt
     2041 cggtggtcgt tgtggcctct atcgatttcc acacaaaaac tttactgttt agtaatagaa
     2101 aagaacttga gaaaggcagc caaaagaatg tacgatctga cgggtaagca tgtctgctat
     2161 gtggctgact gcggtggcat tgcactggag accagcaagg ttctcatgac caagaatata
     2221 gcggtgagtg gtagagtggt gtagagagtg gagagtggtt ctggttctta aacaagtctc
     2281 tagtctcttg gctgctgctg gtagctgtcg aaactaatta agttaatgac tttttcatgt
     2341 tttgtttttg tccgctgact tttgctgttc aaagctacaa ttaagtttat gttttgagtt
     2401 tgatctactt ttgcttaagg gaagtacttt cgattacgga ttgctggcat ataaaaatag
     2461 ataccaagga atgtgcatca tatcatattg attctatctt gattctttgc ccagaaactg
     2521 gccatccttc agagcgtgga aaaccccccg gccatcgctc agttacaatc gattaagcac
     2581 agcacacaga tcttcttctg gaccttcgat gtgaccatgg cccgcgagga gatgaagaag
     2641 tacttcgatg aggtcatggt ccagatggac tacatcgatg tgctgatcaa tggggccacc
     2701 ctgtgcgatg agcggaacat tgatgccacc atcaatacga atctgaccgg catgatgaac
     2761 accgtggcca ctgtgctgcc ctatatggac cgtaagatgg gcggttcggg tggactgatt
     2821 gtgaatgtca cctccgtcat aggactggat ccatcgccgg tcttttgtgc gtacagtgcc
     2881 tcaaagtttg gtgtgattgg gttcacccga agtctagcgg tgagtgggcg agatcttaac
     2941 ttgaaaaggc tctctaatgt cttccattct tcggcaggat cccctgtatt acacccaaaa
     3001 tggtgtggct gtcatggccg tctgctgtgg tcccaccaaa gtgtttgtcg atcgggaact
     3061 gacagccttc ctggcatacg gccaaacctt tgccgatcgc ctgcgtcgtg ccccctgcca
     3121 atcgacggcc gtctgcggcc agaatatagt aaatgccatc gagagatcgg agaacggaca
     3181 gatctggatt gccgacaagg gtggcctgga gtcggtggcc ctccactggt attggcatat
     3241 ggccgatcag tttgtgaact atatgcagag cactgacgat gaagatcagg agtttttttt
     3301 gggccagcga tgaggcatgc cttgatccac ggaataaaac atcaaatgtg tcacaaaaag
     3361 taggcaactt gtttgtgggt ctggaaatag atttgaaata ttttgaggtc atatgaataa
     3421 tgacataaat gagggtgcct gaaagtatgc caagttt
//