From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human gamma-glutamylcysteine synthetase (GCS) mRNA, complete cds.
Date: 11 Sep 92 10:04:28 GMT
Distribution: bionet
Checksum: 38585     6

LOCUS       HUMGCS       2634 bp ss-mRNA            PRI       11-SEP-1992
DEFINITION  Human gamma-glutamylcysteine synthetase (GCS) mRNA, complete cds.
ACCESSION   M90656
KEYWORDS    gamma-glutamylcysteine synthetase.
SOURCE      Homo sapiens (library: statagene) liver cDNA to mRNA.
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 2634)
  AUTHORS   Mulcahy,R.T., Chang,C. and Gipp,J.J.
  TITLE     Cloning and nucleotide sequence of a full-length cDNA for human
            liver gamma-glutamylcysteine synthetase
  JOURNAL   Biochem. Biophys. Res. Commun. 185, 29-35 (1992)
  STANDARD  simple staff_entry
FEATURES             Location/Qualifiers
     CDS             93..2006
                     /EC_number="6.3.2.2"
                     /product="gamma-glutamylcysteine synthetase"
                     /gene="GCS"
                     /codon_start=1
                     /translation="MGLLSQGSPLSWEETKRHADHVRRHGILQFLHIYHAVKDRHKDV
                     LKWGDEVEYMLVSFDHENKKVRLVLSGEKVLETLQEKGERTNPNHPTLWRPEYGSYMI
                     EGTPGQPYGGTMSEFNTVEANMRKRRKEATSILEENQALCTITSFPRLGCPGFTLPEV
                     KPNPVEGGASKSLFFPDEAINKHPRFSTLTRNIRHRRGEKVVINVPIFKDKNTPSPFI
                     ETFTEDDEASRASKPDHIYMDAMGFGMGNCCLQVTFQACSISEARYLYDQLATICPIV
                     MALSAASPFYRGYVSDIDCRWGVISASVDDRTREERGLEPLKNNNYRISKSRYDSIDS
                     YLSKCGEKYNDIDLTIDKEIYEQLLQEGIDHLLAQHVAHLFIRDPLTLFEEKIHLDDA
                     NESDHFENIQSTNWQTMRFKPPPPNSDIGWRVEFRPMEVQLTDFENSAYVVFVVLLTR
                     VILSYKLDFLIPLSKVDENMKVAQKRDAVLQGMFYFRKDICKGGNAVVDGCGKAQNST
                     ELAAEEYTLMSIDTIINGKEGVFPGLIPILNSYLENMEVDVDTRCSILNYLKLIKKRA
                     SGELMTVARWMREFIANHPDYKQDSVITDEMNYSLILKCNQIANELCECPELLGSAFR
                     KVKYSGSKTDSSN"
BASE COUNT      792 a    527 c    609 g    706 t
ORIGIN
        1 ggcacgaggc tgagtgtccg tctcgcgccc ggaagcgggc gaccgccgtc agcccggagg
       61 aggaggagga ggaggaggag gagggggcgg ccatggggct gctgtcccag ggctcgccgc
      121 tgagctggga ggaaaccaag cgccatgccg accacgtgcg gcggcacggg atcctccagt
      181 tcctgcacat ctaccacgcc gtcaaggacc ggcacaagga cgttctcaag tggggcgatg
      241 aggtggaata catgttggta tcttttgatc atgaaaataa aaaagtccgg ttggtcctgt
      301 ctggggagaa agttcttgaa actctgcaag agaaggggga aaggacaaac ccaaaccatc
      361 ctaccctttg gagaccagag tatgggagtt acatgattga agggacacca ggacagccct
      421 acggaggaac aatgtccgag ttcaatacag ttgaggccaa catgcgaaaa cgccggaagg
      481 aggctacttc tatattagaa gaaaatcagg ctctttgcac aataacttca tttcccagat
      541 taggctgtcc tgggttcaca ctgcccgagg tcaaacccaa cccagtggaa ggaggagctt
      601 ccaagtccct cttctttcca gatgaagcaa taaacaagca ccctcgcttc agtaccttaa
      661 caagaaatat ccgacatagg agaggagaaa aggttgtcat caatgtacca atatttaagg
      721 acaagaatac accatctcca tttatagaaa catttactga ggatgatgaa gcttcaaggg
      781 cttctaagcc ggatcatatt tacatggatg ccatgggatt tggaatgggc aattgctgtc
      841 tccaggtgac attccaagcc tgcagtatat ctgaggccag atacctttat gatcagttgg
      901 ctactatctg tccaattgtt atggctttga gtgctgcatc tcccttttac cgaggctatg
      961 tgtcagacat tgattgtcgc tggggagtga tttctgcatc tgtagatgat agaactcggg
     1021 aggagcgagg actggagcca ttgaagaaca ataactatag gatcagtaaa tcccgatatg
     1081 actcaataga cagctattta tctaagtgtg gtgagaaata taatgacatc gacttgacga
     1141 tagataaaga gatctacgaa cagctgttgc aggaaggcat tgatcatctc ctggcccagc
     1201 atgttgctca tctctttatt agagacccac tgacactgtt tgaagagaaa atacacctgg
     1261 atgatgctaa tgagtctgac cattttgaga atattcagtc cacaaattgg cagacaatga
     1321 gatttaagcc ccctcctcca aactcagaca ttggatggag agtagaattt cgacccatgg
     1381 aggtgcaatt aacagacttt gagaactctg cctatgtggt gtttgtggta ctgctcacca
     1441 gagtgatcct ttcctacaaa ttggattttc tcattccact gtcaaaggtt gatgagaaca
     1501 tgaaggtagc acagaaaaga gatgctgtct tgcagggaat gttttatttc aggaaagata
     1561 tttgcaaagg tggcaatgca gtggtggatg gttgtggcaa ggcccagaac agcacggagc
     1621 tcgctgcaga ggagtacacc ctcatgagca tagacaccat catcaatggg aaggaaggtg
     1681 tgtttcctgg actgatccca attctgaact cttaccttga aaacatggaa gtggatgtgg
     1741 acaccagatg tagtattctg aactacctaa agctaattaa gaagagagca tctggagaac
     1801 taatgacagt tgccagatgg atgagggagt ttatcgcaaa ccatcctgac tacaagcaag
     1861 acagtgtcat aactgatgaa atgaattata gccttatttt gaagtgtaac caaattgcaa
     1921 atgaattatg tgaatgccca gagttacttg gatcagcatt taggaaagta aaatatagtg
     1981 gaagtaaaac tgactcatcc aactagacat tctacagaaa gaaaaatgca ttattgacga
     2041 actggctaca gtaccatgcc tctcagcccg tgtgtataat atgaagacca aatgatagaa
     2101 ctgtactgtt ttctgggcca gtgagccaga aattgattaa ggctttcttt ggtaggtaaa
     2161 tctagagttt atacagtgta catgtacata gtaaagtatt tttgattaac aatgtatttt
     2221 aataacatat ctaaagtcat catgaactgg cttgtacatt tttaaattct tactctggag
     2281 caacctactg tctaagcagt tttgtaaatg tactggtaat tgtacaatac ttgcattcca
     2341 gagttaaaat gtttactgta aatttttgtt cttttaaaga ctacctggga cctgatttat
     2401 tgaaattttt ctctttaaaa acattttctc tcgttaattt tcctttgtca tttcctttgt
     2461 tgtctacatt aaatcacttg aatccattga aagtgcttca agggtaatct tgggtttcta
     2521 gcaccttatc tatgatgttt cttttgcaat tggaataatc acttggtcac cttgccccaa
     2581 gctttcccct ctgaataaat acccattgaa ctctgaaaaa aaaaaaaaaa aaaa
//