From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: E. coli protease II gene, complete cds.
Date: 21 Dec 92 20:26:58 GMT
Distribution: bionet
Checksum: 27591 6

LOCUS       ECOPT2       2339 bp ds-DNA             BCT       18-NOV-1992
DEFINITION  E. coli protease II gene, complete cds.
ACCESSION   D10976 D01148
KEYWORDS    protease II.
SOURCE      Escherichia coli (strain HB101) DNA.
  ORGANISM  Escherichia coli
            Prokaryotae; Gram-negative facultatively anaerobic rods;
            Enterobacteriaceae.
REFERENCE   1  (bases 1 to 2339)
  AUTHORS   Kanatani,A., Masuda,T., Shimoda,T., Misoka,F., Lin,X.S.,
            Yoshimoto,T. and Tsuru,D.
  TITLE     Protease II from Escherichia coli: Sequencing and expression of the
            Enzyme gene and characterization of the expressed Enzyme
  JOURNAL   J. Biochem. 110, 315-320 (1991)
  STANDARD  full automatic
COMMENT     Submitted (01-July 1991) to DDBJ by: Tadashi Yoshimoto School of
            Pharmaceutical Sciences Nagasaki University 1-14 Bunkyo-machi
            Nagasaki 852 Japan Phone:  0958-47-1111 x2551 Fax:
            0958-44-6774.
FEATURES             Location/Qualifiers
     -35_signal      50..55
     -10_signal      80..85
     RBS             95..100
     CDS             110..2233
                     /EC_number="3.4.21.14"
                     /gene="ptrII"
                     /product="protease II"
                     /codon_start=1
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWIFYYVRKHPVTLLPYQVWRHAIGTPASQDKLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFYAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDDHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPL
                     RLRTKYFPDNVSVLNAAPGSCCPGY"
BASE COUNT      579 a    576 c    587 g    597 t
ORIGIN      
        1 atccgcgcca ttacctgtca tcatctaagc aatgactccc ctgtttcgct tgcatccccg
       61 gtgagttttg ccacccttat aagatgtttc aaccagaaag aacaataaca tgctaccaaa
      121 agccgcccgc attccccacg ccatgacgct tcatggcgat acgcgcatcg ataattacta
      181 ctggctgcgg gacgatacgc gttctcagcc agaagtcctg gactacctgc aacaagaaaa
      241 tagttacggt catcgggtga tggcctcaca acaagccttg caggatcgca tcttaaagga
      301 aatcatcgac cgcattccgc aacgagaagt ttctgcgccc tacatcaaaa atggctaccg
      361 ctatcggcat atttatgaac caggctgtga atatgctatc taccagcgtc aatcggcatt
      421 cagtgaagag tgggatgagt gggaaacatt gctcgatgcc aataagcgcg cagctcatag
      481 tgagttttat tcgatgggcg gaatggcgat tacgcccgat aacaccatta tggcgctggc
      541 agaagatttt ctttcccgac gccagtacgg cattcgtttt cgtaatctgg aaactggtaa
      601 ctggtacccg gaactgctgg ataacgttga acccagcttt gtctgggcaa atgactcctg
      661 gattttctac tatgttcgca agcatccggt gacgctgctg ccttatcagg tctggcgtca
      721 cgccatcggt acgccagcat cgcaagataa actgatctac gaagaaaaag acgataccta
      781 ttacgtcagc ctgcataaaa cgacctcgaa gcactatgta gtcattcatt tggccagcgc
      841 caccaccagt gaagttcgcc tgctggacgc ggaaatggcc gatgccgagc cgtttgtttt
      901 tctgccgcgc cgcaaagatc acgaatacag ccttgatcac taccagcatc gtttttatct
      961 gcgttccaac cgccacggca aaaactttgg cttataccgt acccgtatgc gtgatgagca
     1021 acagtgggaa gagttaattc cgccacgcga aaacatcatg ctggaagggt ttacgctgtt
     1081 taccgactgg ctggtggttg aagagcgtca gcgcgggtta accagtttgc gccaaattaa
     1141 ccgcaagacc cgggaagtca ttggtattgc ctttgatgat ccggcctatg tgacctggat
     1201 tgcctacaat ccagaacctg aaaccgcgcg attgcgttat ggttattctt ccatgactac
     1261 accagacact ttgtttgaac tggatatgga taccggtgag cgtcgtgtat taaaacaaac
     1321 ggaagttcct ggtttttatg cggcgaatta ccgcagtgaa cacctgtgga tagtcgcccg
     1381 tgatggcgtc gaagttccgg tttcgttggt ctaccatcgc aaacattttc gcaaaggaca
     1441 caacccgttg ctggtgtatg gctatggttc ttacggcgca agtattgatg ccgatttcag
     1501 ttttagccgc ttgagtttgt tagatcgtgg ctttgtctac gccattgtcc atgttcgcgg
     1561 cggtggtgag ctggggcaac aatggtacga agacggaaaa tttctgaaga agaaaaatac
     1621 gtttaatgat tatcttgatg cctgcgatgc attgttaaaa ctgggctatg gctctccttc
     1681 gctttgttat gcgatgggcg ggagtgcggg gggcatgttg atgggcgttg caattaatca
     1741 acgcccggaa ttattccacg gcgttatcgc ccaggtaccg tttgttgatg ttgtaacaac
     1801 gatgcttgat gaatcaattc ctcttaccac tggtgagttt gaagagtggg gtaacccgca
     1861 ggatccgcaa tattacgagt acatgaaaag ctacagcccg tatgacaacg tcaccgcaca
     1921 ggcttatccg catttactgg taacgaccgg tttgcacgat tctcaggtgc aatattggga
     1981 accggcaaaa tgggtcgcta aattgcgcga gctgaaaacc gatgaccatc ttttattgct
     2041 ctgtaccgac atggactcag gccatggcgg taaatctggt cgctttaaat cgtacgaagg
     2101 cgtagcgatg gaatatgctt ttctggtcgc gctggcgcag ggaacattac ccctacgcct
     2161 gcggactaag tattttccag ataatgtttc agtgttaaac gcagctccgg gctcatgctg
     2221 tccaggttat taaataacca gcgcagatag cccggatcgc gttcgccaac gtcggaaacc
     2281 gctttgccac ggtatttgcc aaaggtgaag gtcgtcatca acgacggacg tccggtgat
//