From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Pseudomonas sp. DL-hydantoinase (hyuR) gene, 3' end and (hyuA,
Date: 25 Jul 92 10:05:43 GMT
Distribution: bionet
Checksum: 12916    15

LOCUS       PSEHYURABC   7440 bp ds-DNA             BCT       25-JUL-1992
DEFINITION  Pseudomonas sp. DL-hydantoinase (hyuR) gene, 3' end and (hyuA,
            hyuB, hyuC) genes, complete cds.
ACCESSION   M72717
KEYWORDS    DL-hydantoinase; N-carbamyl-L- amino acid amidohydrolase.
SOURCE      Pseudomonas sp. (strain NS671) DNA.
  ORGANISM  Pseudomonas sp.
            Prokaryotae; Gracilicutes; Scotobacteria; Pseudomonadaceae.
REFERENCE   1  (bases 1 to 7440)
  AUTHORS   Watabe,K.
  TITLE     Cloning and sequencing of genes involved in conversion of
            5-substituted hydantoins to corresponding L-amino acids from a
            native plasmid of Pseudomonas sp. strain NS671
  JOURNAL   J. Bacteriol. 174, 962-969 (1992)
  STANDARD  full staff_entry
FEATURES             Location/Qualifiers
     CDS             <1..888
                     /product="DL-hydantoinase"
                     /gene="hyuR"
                     /codon_start=1
                     /translation="DQEIVVKSMAMEKVYELACRVADSDATIFLQGETGVGKEVLART
                     IHNSSIRKEAPFIKVNCGAIPESIMESELFGYSKGTFTGGNKDGKKGLAQAAHNGTLF
                     LDEIGELPLNLQAKLLQLLNEKQFTPLGEIKPVQVDVRFIAATNRNLEDMVREGTFRE
                     DLYYRLFVIPITIPSLSERREDIPFLINHFLETFNHKYKLYKTIDKEVVQFFIDYEWK
                     GNVRELQNTIERLVLISSAQQIELSDLSDKFKKATSHTKGGISGEGLNLKQKMEQFEK
                     QILIQTLESSNTMKEASKN"
     promoter        1099..1126
                     /type="sigma 70"
                     /gene="hyuA"
     promoter        1099..1115
                     /type="sigma 54"
                     /gene="hyuA"
     CDS             1156..3228
                     /product="DL-hydantoinase"
                     /gene="hyuA"
                     /codon_start=1
                     /translation="MKLFGVDVGGTFTDIIFSDTETRVTAIHKVPTTLDDPSTGVVQG
                     ILELCDRQYIDRTAIDHVFHGTTIATNAILEYDGAKTGMITTEGYRDIIHIGRHQRPQ
                     NYSIMQEIPWQDRPLVQRRHRLAIAERMGPVKGQVITPVQEDQVRGAVATLKERGVDS
                     IIVNFLFSYTNPEHEQRVKEIIEEEYPEAFVTISSEVSPQFREFERFTTASINGFVGP
                     KVKNYIQNLEQSLKDSGISAELHIMCSNGGVATPKTVSEKPVNTLLSGPAAGILGGAW
                     AGELTNRQKLITFDVGGTSADIGIITDSGYGESSARDTWIAGYPVMVPMIDIHTIGAG
                     GGSIAHIDEGGAFKVGPRSAGSRPGPACYGHGGLKPTVSDANVVLGRIDEHNFLGGEM
                     KIYTNAAYKVIDELAGQLDLSRERTAEGVLQIMNNNMANAIREKTIQKGEDPREFSLV
                     AFGGAGPLHAVEVAQILNIPEVIIPLYPGINSATGLLTTDLKYDVIKTEFMMSTNMDF
                     SGLNEDLAGLETQLINQLKEDGVSKQDIRILRSADCRYAGQGYELRVDLPDVFLDEET
                     IVDALNNFHESHKAEYGHNFTDSPIEFVNIRVTGTGYMPKIEKQAIHHEYQLEDALLK
                     TGDATFNIDGSLVKVEINFYQREKIPVGAEFNGPCIVLQKDTTTVIPPNCTAYIEEYG
                     NMIIKVGV"
     CDS             3232..5010
                     /product="DL-hydantoinase"
                     /gene="hyuB"
                     /codon_start=1
                     /translation="MSKIHTDLKKIDPITVQVVLGSLENVAVEMGHKLARMSYSSIIR
                     ESEDFGCALVDVRGQQLCESSHSTPLQSGPIPGYIKGIREIMEDRNDTFNQGDVIMHN
                     SPYHGASHGPDVGFCIPVFYKDELIGFSVTTAHHLDIGSSTPGSCGIVDAVDAYAEGL
                     QFKAIKVYDQGVKNRYVWDILKDNIRAPKLVVGDMEAQIAAARIGAQRYIEIIEKYGL
                     DTVQAASEELMNYSEKMMRDAIKKLPDGEYTAEGFLDGYLDSDDPAKKDLRINVTVKV
                     DGSDLTVDLTGTSPQVTDKPINMPLLGTVDIAIYLTLRSILLDSTVYGNFPQNSGLIR
                     PIKIVAPKGTLCNPIFPAPTIARFNSGNAVADTLMKALAQVVPHQVSAGVGNLQVVAF
                     SGQSNENYWVYMDIMEGSYGGRYGKDGMDAVDTLYANTRNNPIEDIESHYPLRVNRYE
                     LRDNDSAPGKWRGGIGSIREVSFLADGSFSVEADGHKYAPWGFDDGQDGYVGSLSIRD
                     NETNELVQLPSKLPNRHAQSGSTIQLVGPCGGGYGNPLEREPEKVLSDYLDGFITKEK
                     ALVEYGVTITDSEEIDYEKTNELRKV"
     CDS             5031..6275
                     /product="DL-hydantoinase"
                     /gene="hyuC"
                     /codon_start=1
                     /translation="MKTVTISKERLRIHIEQLGEIGKTKDKGVQRLALSKEDREATLL
                     VSEWMREAGLTVTHDHFGNLIGRKEGETPSLPSVMIGSHIDSVRNGGKFDGVIGVLAG
                     IEIVHAISEANVVHEHSIEVVAFCEEEGSRFNDGLFGSRGMVGKVKPEDLQKVDDNNV
                     TRYEALKTFGFGIDPDFTHQSIREIGDIKHYFEMHIEQGPYLEKNNYPIGIVSGIAGP
                     SWFKVRLVGEAGHAGTVPMSLRKDPLVGAAEVIKEVETLCMNDPNAPTVGTVGRIAAF
                     PGGSNIIPESVEFTLDIRDIELERRNKIIEKIEEKIKLVSNTRGLEYQIEKNMAAVPV
                     KCSENLINSLKQSCKELEIDAPIIVSGAGHDAMFLAEITEIGMVFVRCRNGISHSPKE
                     WAEIDDILTGTKVLYESIIKHI"
     promoter        6301..6316
                     /type="sigma 54"
                     /note="ORF 5"
     promoter        6301..6328
                     /type="sigma 70"
                     /note="ORF 5"
     CDS             6741..>7440
                     /note="ORF 5"
                     /codon_start=1
                     /translation="MPTVNKERNMSSIAYFILWVGIAVQLVTPITAAQLYPALSPLQI
                     IIACILGNLIVAILLTLLGDIGVRYGIPYAVYIRASFGYLGAHIPGIVRAVPAVFWFG
                     FQTWMGAYALDAIMEMLTGYSNLTLLIILFGVVQIINTAMGMEAITKFEWLASPSILI
                     IGIILQVYIMKQHHLTFSEIFSHGGDGGVSMGYAVVVMMGTYITMALNAPDFTRFLKT
                     KTGSSEPNWWKVNKG"
BASE COUNT     2417 a   1230 c   1691 g   2102 t
ORIGIN
        1 gatcaagaaa tagttgttaa atccatggct atggaaaagg tttatgaact ggcatgtaga
       61 gtagccgatt cggatgccac catttttctg caaggggaaa caggtgtagg taaggaagtt
      121 ctggctagaa cgattcataa ttcaagcatt agaaaagaag ctccgtttat aaaagtgaat
      181 tgtggagcaa ttccggaaag tattatggaa tctgagcttt ttggttattc aaaaggtact
      241 ttcaccggtg ggaataaaga tggaaaaaag gggttagccc aggcagcgca taatggaact
      301 ttatttttag atgagattgg tgaattacca cttaatttac aggcgaaatt gcttcaactt
      361 ttaaatgaaa agcagttcac tccacttgga gaaataaagc cagtacaagt agatgttcga
      421 tttatagctg cgaccaatcg taatttagag gatatggtga gagagggaac ttttcgtgaa
      481 gacctatatt atcgtttatt tgttatacct attactattc cctctttatc agaacgaaga
      541 gaggacatac cctttctaat caaccatttt ttagaaacat ttaaccataa atacaaatta
      601 tataaaacaa ttgataagga agtagtacag tttttcatag attatgaatg gaagggaaat
      661 gtaagagaat tacaaaatac tattgaaaga ctcgttctta tatcatcagc tcagcaaata
      721 gagttaagtg atctatcaga taaatttaaa aaggccactt ctcacactaa aggaggaatc
      781 agtggcgaag gtttgaatct aaagcaaaaa atggagcaat ttgaaaagca aatccttatt
      841 caaactttag aaagctctaa tacaatgaaa gaagcaagta aaaattagga gtggatgcat
      901 cgacaattac cagaaaagtt aaaaaataca acataacgtg caaatgcaat ttctattgtg
      961 atttgcaatt tttattttaa gcctagtttt atgaattttc tgatataaat tatggatgtt
     1021 agttcattta ggattgcacc tttgcaattt cgatttattc tcaatcttaa aaagctattt
     1081 ttatcagctt tttatacttt ggcatgaaac ttgcggtaaa taatattaga acacaattta
     1141 aagggggaaa gtgttatgaa attatttggc gtagacgtcg gaggaacttt taccgatata
     1201 atttttagcg atactgaaac acgggtgaca gctattcata aagtcccaac aacattggac
     1261 gatccatcaa ccggtgtggt tcaaggtatt cttgaacttt gtgaccgtca atatattgac
     1321 agaactgcaa ttgatcatgt gtttcacgga acaacgattg ccactaatgc gattcttgag
     1381 tatgacgggg caaaaacagg gatgatcaca acagaaggat acagggatat catacatatc
     1441 gggaggcatc aacgaccaca aaactattcc atcatgcaag aaattccatg gcaggataga
     1501 ccattagtcc agcgaagaca tcgattagcg attgcagagc gtatgggccc agttaaaggc
     1561 caggtcatta ctccagtaca ggaagatcaa gttcggggtg cggttgcaac gttaaaggaa
     1621 aggggcgttg attctattat tgttaacttc ttattttcct ataccaaccc tgagcatgaa
     1681 caacgtgtaa aagaaataat cgaagaagaa tatccggaag cattcgtcac gatttcttct
     1741 gaagtttcac cgcaatttcg tgagtttgaa cgttttacca cagcttccat taacggcttt
     1801 gtggggccga aggtgaaaaa ttacattcaa aacttggaac agagtttgaa agattcagga
     1861 atctctgctg aacttcatat tatgtgctca aacggtggag tagcaacgcc taaaactgta
     1921 tctgaaaagc ctgtcaatac gctgttgtca gggcctgcag caggtatctt gggaggagct
     1981 tgggccgggg aattaacaaa tcgtcaaaaa ctgattacct ttgacgtagg cggcactagt
     2041 gctgacatag gtattatcac tgatagcggt tatggcgaat cgtcagctcg tgatacatgg
     2101 atagccggtt atccagtcat ggtaccgatg attgatattc atacaatcgg cgcaggtggc
     2161 ggcagtatag cccatattga tgaaggcggt gcatttaagg ttgggccaag aagtgcaggt
     2221 tctcgtccag gaccagcctg ttacggccat ggcgggttaa agccaacagt aagtgatgct
     2281 aacgttgtgc tcggaaggat tgatgaacat aatttccttg gtggagagat gaaaatctat
     2341 acaaatgcag catacaaggt aatagacgaa ttagcgggac aattggattt aagtagagaa
     2401 agaacagctg aaggtgtctt gcaaataatg aataacaaca tggcaaatgc gattcgtgaa
     2461 aaaacgattc aaaaaggcga ggacccgagg gagttctcat tggttgcttt tggcggagcc
     2521 ggtccgctac atgccgtaga agtcgctcag attttaaata taccagaggt aattataccg
     2581 ttatacccag gaatcaactc cgccactgga ttattaacga cagatttaaa atatgacgtt
     2641 atcaaaacgg aatttatgat gagtactaat atggatttct ccggcttgaa cgaagattta
     2701 gctggattag aaacccagct tattaatcaa ctaaaagaag acggagtctc caaacaagat
     2761 attcgcattc ttcgaagtgc tgattgccga tatgccggtc aggggtatga gttgcgtgtt
     2821 gatctgccag acgtatttct tgatgaagaa actattgtcg atgcattgaa caattttcat
     2881 gagagccata aagcagagta tggacataat tttacagaca gcccaattga gtttgtcaat
     2941 attcgcgtaa ctggaactgg ctacatgccg aaaatcgaaa agcaggcgat ccatcacgag
     3001 tatcaattgg aagatgcatt gttaaaaaca ggtgatgcta cttttaatat cgatggaagc
     3061 ctagtaaaag tcgaaatcaa tttctatcaa agagagaaga ttcctgttgg tgctgaattt
     3121 aatggtcctt gtattgtgct acaaaaagat acaaccactg tcattcctcc taactgtact
     3181 gcttatatcg aggaatacgg aaatatgatt attaaggtag gtgtttaatt tatgtcaaaa
     3241 atccatactg acttaaagaa gatcgaccca attacggttc aggttgttct aggttcttta
     3301 gaaaatgttg cggttgaaat gggacacaag cttgcccgga tgtcttattc cagtattatt
     3361 cgggaatctg aagactttgg ttgtgcgcta gttgatgtca gaggacaaca gctatgtgag
     3421 tcctctcaca gtacacctct tcaatctggt ccgattccgg gatatataaa ggggatccgt
     3481 gaaatcatgg aggatcgcaa cgataccttc aatcagggtg atgtcattat gcataattca
     3541 ccatatcatg gagcttccca cggtccagat gtaggatttt gtattccggt gttttataag
     3601 gacgaattaa ttggattctc tgttacgacg gcacaccatt tagatatagg atcatcaact
     3661 ccaggaagct gtgggatcgt agatgcggta gatgcctatg ccgaaggcct tcaatttaaa
     3721 gctataaaag tctatgacca aggtgttaaa aaccgttatg tatgggatat tttgaaagat
     3781 aacatacgcg ctccgaaatt agtcgttggt gatatggaag cccaaatagc agccgctaga
     3841 atcggtgcac aaaggtatat agagattatt gaaaaatatg gattagacac cgttcaagca
     3901 gcaagtgaag aactgatgaa ctactcagaa aaaatgatgc gggatgccat taaaaagctg
     3961 ccagatggag aatacactgc tgaaggtttt ttggatggat atttagacag tgacgatcct
     4021 gctaaaaaag atttaagaat caatgtaaca gtgaaggttg atgggagtga tttgacagtc
     4081 gatttgactg ggacatctcc tcaagtgact gacaaaccaa tcaatatgcc cttattagga
     4141 acagtcgata ttgctatcta tcttacatta cggtcaatct tactagattc cacagtatat
     4201 gggaatttcc cgcaaaattc agggttaatc cggccaatta agattgtggc accgaaaggt
     4261 acattatgta atccaatctt tcctgcgccg acgattgctc gattcaattc tgggaatgca
     4321 gttgctgata ctttaatgaa agctttagca caggttgttc cgcatcaggt cagtgctggt
     4381 gtcggcaacc ttcaggttgt ggcattcagc ggtcaaagta atgagaatta ctgggtttat
     4441 atggatatca tggaagggag ctatggaggt cgctacggca aggatggaat ggatgcagta
     4501 gatactttat acgctaacac gagaaataat ccgatagaag atattgaatc tcactatcct
     4561 ttaagagtaa atcgatatga gctaagagat aatgatagtg ctccagggaa gtggcgcgga
     4621 ggaataggat ccattcgtga ggtcagtttc ttggctgatg gaagtttctc tgttgaagcg
     4681 gatggacata aatatgcacc gtggggattt gatgacgggc aagacggcta tgtcggaagc
     4741 ttgtctattc gagacaatga aacaaatgaa ctcgtccaat tgccttccaa gctccctaat
     4801 agacacgctc aatccggaag caccattcaa ttagtaggcc catgtggagg aggttatggc
     4861 aatccacttg aaagggagcc tgagaaagtt ctatcagact atttagatgg atttattaca
     4921 aaggaaaaag ctctagtgga atatggagtc acgattactg attcagagga aatagattat
     4981 gagaaaacga acgagttaag aaaggtttaa agttaaaaat ggaggacgct atgaaaacag
     5041 taactattag taaagaaagg ttacgcatcc atatagaaca actaggtgaa ataggaaaga
     5101 cgaaagataa gggcgtacaa cgtcttgcat tatccaagga agatagagaa gctaccctgc
     5161 tagtttctga atggatgaga gaggccggcc ttactgttac tcatgatcat tttgggaatt
     5221 taattggacg gaaagaggga gaaaccccaa gccttccttc cgtaatgatc ggttctcata
     5281 ttgattctgt tcgaaatggc ggtaagtttg atggggttat tggagtactg gcagggattg
     5341 aaattgtcca cgccatatcc gaggcaaatg tcgttcatga acattcaatt gaagtggtag
     5401 ctttttgtga agaggaaggt tcaaggttta atgacggatt attcggaagt agaggcatgg
     5461 ttggaaaggt aaaacccgag gatttacaaa aagttgacga taacaatgta actagatatg
     5521 aagcgctaaa aacctttggc tttgggatag atcctgattt tacccaccaa tctatacggg
     5581 agattggtga cattaaacat tattttgaga tgcatattga gcaagggcca tatctagaaa
     5641 agaataatta cccaataggg attgtaagtg ggattgcggg tccttcttgg ttcaaggtaa
     5701 ggctagttgg agaagctggc catgcgggga ctgttcctat gagtctacgc aaagaccccc
     5761 tagtcggggc tgcggaagtc attaaagaag tagaaactct ttgtatgaac gatccaaatg
     5821 ctcccacagt cggcactgtt ggtagaatag cggcttttcc tggaggaagt aatattattc
     5881 ctgaatcagt ggagtttacc cttgatatca gggatattga actggagaga cgaaacaaaa
     5941 ttattgagaa gatagaagaa aagatcaaac ttgtaagtaa caccagagga ttggaatatc
     6001 agattgaaaa aaacatggct gcagttcctg tgaaatgctc tgaaaatcta atcaactcct
     6061 taaaacaatc atgcaaggag ctggagatag atgcaccgat tatcgttagt ggtgcaggac
     6121 acgatgcgat gtttttagcg gaaataacag aaataggaat ggtgtttgtc cgttgccgta
     6181 atggtataag ccactcacca aaagagtggg cggaaataga tgatattctc actggaacta
     6241 aggtactgta tgaatcaatt ataaaacata tataaactgt tgtatggcgg tctgtatgga
     6301 ctgccataca attgaataat tataatattt attttcggaa aattcttaat ttttaaaaat
     6361 atttttttta gaattcacga acaaaggaat tagtacacct attgaaggta aatgaagtgg
     6421 actaaatctc aaaacttcga atcttagaat acttactgat aggtaaaaaa ataattgtta
     6481 attaactatt tgaaagtgaa ttattgatgt taggccccaa catcaacgtt attttctcca
     6541 taagtattag aaatggtttt gctcttagac aatcatcaca ataaaactag taacacaaac
     6601 aaaatgtctg ttatgagaga atcaatcgga acattatgaa gaacttcaaa gaagatttct
     6661 ttctaaggaa aaattaaaag gaggtaattt ctttgaataa cgttaatgaa aaggggatta
     6721 gtttaaaggg aaaagacatc atgccgacag tgaacaagga aagaaatatg tcctcaatag
     6781 cgtattttat tttatgggtt gggatagctg tacaactagt aacccccata acagcggctc
     6841 agctttatcc agcattatca cccctgcaaa tcatcatagc ttgtatcctt ggtaatctaa
     6901 ttgttgctat tcttttaaca ttattagggg atattggggt gagatatgga attccatatg
     6961 cggtttatat tagggcgagc ttcggttatt taggagccca tataccaggt attgtacgtg
     7021 ccgttccagc ggtattctgg tttggttttc aaacatggat gggtgcatat gccttggatg
     7081 caatcatgga aatgttaacg ggttactcga accttacatt gttaattatt ttatttggtg
     7141 tagtgcaaat cattaacact gccatgggaa tggaagctat tacaaaattt gaatggctgg
     7201 cttcccctag tattttaatt ataggtatta ttttacaagt atatattatg aaacagcatc
     7261 atttaacatt cagtgagatt ttctcccatg ggggtgacgg tggtgtttca atgggctatg
     7321 ctgtcgtcgt aatgatggga acatatatta cgatggcttt aaatgccccg gattttaccc
     7381 ggttcttaaa gacaaaaact ggtagtagtg agcctaattg gtggaaagtc aataaaggaa
//