From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: S.bicolor chloroplast rpoC2 gene (C2.2).
Date: 12 Sep 92 10:03:49 GMT
Distribution: bionet
Checksum: 54174     4

LOCUS       SRGSBRPO22   1142 bp ds-DNA             PLN       12-SEP-1992
DEFINITION  S.bicolor chloroplast rpoC2 gene (C2.2).
ACCESSION   Z14984
KEYWORDS    rpoC2 gene.
SOURCE      Sorghum bicolor DNA.
  ORGANISM  Sorghum bicolor
            Eukaryota; Plantae; Embryobionta; Magnoliophyta; Liliopsida;
            Commelinidae; Cyperales; Poaceae.
REFERENCE   1  (bases 1 to 1142)
  AUTHORS   Chen,Z.
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
REFERENCE   2  (bases 1 to 1142)
  AUTHORS   Chen,Z.
  TITLE     A chloroplast DNA deletion located in RNA polymerase gene rpoC2 in
            CMS lines of sorghum
  JOURNAL   Mol. Gen. Genet. (1992) In press
  STANDARD  full automatic
COMMENT     *source: strain=KS 39A; *source: tissue=Leaf; *source:
            clone_library=cpDNA library; *source: clone=2-92 95 and 2-92;
            *source: is_macronuclear=N; *source: is_proviral=N; *source:
            is_germline=N;
            Mutant of rpoC2.1; differs by 1bp conflict and 165bp internal
            deletion.
            From EMBL    entry CHSBRPO22;  dated 25-AUG-1992.
FEATURES             Location/Qualifiers
     CDS             <1..>1142
                     /partial
                     /gene="rpoC2"
                     /codon_start=2
                     /translation="SLILVQNDQYVESEQVIAEIRAGTSTLHFKEKVQKHIYSESDGE
                     MHWSTDVYHAPEYQYGNLRRLPKTSHLWILSVSMCRSSIASFSLHKDQDQMNTYSFSV
                     DGRYIFDFSMANDQVSHRLLDTFGKKDREILDYLTPDRIVSNGHWNCFYPSILQDNSD
                     LLAKKRRNRFAVPLQYHQEQEKERISCLGISMEIPFMGVLRRNTIFAYFDDPRYRKDK
                     RGSGIVKFRYRTLEEEYRTREEEYRTREEDSEDEYESPENKYRTREGEGEYEILEEDS
                     EEEYGSSEDGSEKEYGTLEEDSEEDSEEDSEDEYGSPEEDSILKKEGFIEHRGTKEFS
                     LKYQKEVDRFFFILQELHILPRSSSLKVLDNSIIGVDTQLTKNTRS"
     conflict        39..41
                     /note="replace(39..41,'ctg')"
                     /citation=[2]
                     /citation=[1]
                     /partial
     misc_feature    819
                     /note="165bp deletion"
BASE COUNT      405 a    179 c    254 g    304 t
ORIGIN
        1 aagcttgatt ctagtgcaaa atgatcaata tgtagaatca gaacaagtaa ttgcggagat
       61 tcgtgccgga acgtccactt tgcattttaa agaaaaggta caaaagcata tttattccga
      121 atcagacggg gaaatgcact ggagtactga tgtttaccat gcgcccgaat atcaatatgg
      181 taatcttcgt cgattaccaa aaacaagcca tttatggata ttgtcagtaa gtatgtgcag
      241 atccagtata gcatcttttt cactgcacaa ggatcaagat caaatgaata cttattcttt
      301 ttctgttgat ggaagatata tctttgactt ctcaatggct aatgatcaag taagccatag
      361 actgttggat acttttggta aaaaagatag ggaaattctt gattatttaa cgccagatcg
      421 aatcgtgtcc aacggtcatt ggaattgttt ctatccttct attcttcaag ataattcgga
      481 tttgttggcg aaaaagcgaa gaaataggtt tgccgttcca ttacaatatc atcaagaaca
      541 agaaaaagag cgaatatcct gtttgggtat ttcaatggaa atacccttta tgggtgtttt
      601 acgtagaaat actatttttg cttattttga cgatccacga tacagaaaag ataaaagggg
      661 ttcaggaatt gttaaattta gatataggac cctagaggaa gaatatcgga cccgagagga
      721 agagtatagg actcgagagg aagactcaga agacgaatat gagagcccag agaacaaata
      781 taggacccga gagggagagg gcgaatatga aatcctagag gaagactcag aagaagaata
      841 tgggagctct gaagatggct cagagaagga atatgggact ttagaagaag actcagagga
      901 agactcagag gaagactcag aagacgaata tgggagcccg gaggaagatt ctatcttaaa
      961 aaaagagggt tttattgagc atcgaggaac aaaagaattt agtctaaaat accaaaaaga
     1021 agtagatcgg ttttttttca ttcttcaaga actgcatatc ttgccgagat cctcatccct
     1081 aaaggtactt gacaatagta ttattggagt cgatacacaa ctcacaaaaa atacaaggtc
     1141 ga
//