From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Peptostreptococcus magnus protein L gene, complete cds.
Date: 8 Jan 93 05:49:21 GMT
Distribution: bionet
Checksum: 32263 9

LOCUS       PEPLPRTN     2657 bp ds-DNA             BCT       07-JAN-1993
DEFINITION  Peptostreptococcus magnus protein L gene, complete cds.
ACCESSION   M86697
KEYWORDS    protein L.
SOURCE      Peptostreptococcus magnus (strain 312) DNA.
  ORGANISM  Peptostreptococcus magnus
            Prokaryotae; Firmicutes; Firmibacteria; Gram-positive cocci;
            Peptococcaceae.
REFERENCE   1  (bases 1 to 2657)
  AUTHORS   Kastern,W., Holst,E., Nielsen,E., Sjobring,U. and Bjorck,L.
  TITLE     Protein L, a bacterial immunoglobulin-binding protein and possible
            virulence determinant
  JOURNAL   Infect. Immun. 58, 1217-1222 (1990)
  STANDARD  full automatic
REFERENCE   2  (bases 1 to 2657)
  AUTHORS   Bjoerck,L., Sjoebring,U. and Kastern,W.
  TITLE     Structure of peptostreptococcal protein L and identification of
            repeated immunoglobulin light chain-binding domain
  JOURNAL   J. Biol. Chem. 267, 12820-12825 (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     misc_feature    301..2025
                     /note="This feature applies to a gene which lacks a coding
                     region feature. "
                     /gene="protein L"
     sig_peptide     301..354
                     /gene="protein L"
                     /codon_start=1
     mat_peptide     355..2457
                     /function="binds kappa chain of immunoglobulins."
                     /product="protein L"
                     /evidence="EXPERIMENTAL"
                     /codon_start=1
                     /citation=[2]
     repeat_region   591..1685
                     /gene="protein L"
     repeat_unit     591..818
                     /function="one of five kappa-binding domains"
                     /rpt_family="B family in protein L gene"
                     /rpt_type=direct
                     /gene="protein L"
                     /evidence="EXPERIMENTAL"
                     /citation=[1]
                     /citation=[2]
     repeat_unit     819..1034
                     /function="one of the five kappa-binding domains"
                     /rpt_family="B family in protein L gene"
                     /rpt_type=direct
                     /gene="protein L"
                     /evidence="EXPERIMENTAL"
                     /citation=[2]
     repeat_unit     1035..1250
                     /function="one of the five kappa-binding domains"
                     /rpt_family="B family in protein L gene"
                     /rpt_type=direct
                     /gene="protein L"
                     /evidence="EXPERIMENTAL"
                     /citation=[2]
     repeat_unit     1251..1466
                     /function="one of the five kappa-binding domains"
                     /rpt_family="B family in protein L gene"
                     /rpt_type=direct
                     /gene="protein L"
                     /evidence="EXPERIMENTAL"
                     /citation=[2]
     repeat_unit     1467..1685
                     /function="one of the five kappa-binding domains"
                     /rpt_family="B family in protein L gene"
                     /rpt_type=direct
                     /gene="protein L"
                     /evidence="EXPERIMENTAL"
                     /citation=[2]
     repeat_unit     1711..1866
                     /function="region of unknown function"
                     /rpt_family="C family in protein L gene"
                     /rpt_type=direct
                     /gene="protein L"
                     /citation=[2]
                     /note="putative"
     repeat_region   1711..2025
                     /gene="protein L"
     repeat_unit     1870..2025
                     /function="region of unknown function"
                     /rpt_family="C family in protein L gene"
                     /rpt_type=direct
                     /gene="protein L"
                     /evidence="EXPERIMENTAL"
                     /citation=[2]
     CDS             301..2460
                     /function="Binds kappa chain of immunoglobulins."
                     /note="putative"
                     /product="protein L"
                     /codon_start=1
                     /translation="MAALAGAIVVTGGVGSYAADEPIDLEKLEEKRDKENVGNLPKFD
                     NEVKDGSENPMAKYPDFDDEASTRFETENNEFEEKKVVSDNFFDQSEHPFVENKEETP
                     ETPETDSEEEVTIKANLIFANGSTQTAEFKGTFEKATSEAYAYADTLKKDNGEYTVDV
                     ADKGYTLNIKFAGKEKTPEEPKEEVTIKANLIYADGKTQTAEFKGTFEEATAEAYRYA
                     DALKKDNGEYTVDVADKGYTLNIKFAGKEKTPEEPKEEVTIKANLIYADGKTQTAEFK
                     GTFEEATAEAYRYADLLAKENGKYTVDVADKGYTLNIKFAGKEKTPEEPKEEVTIKAN
                     LIYADGKTQTAEFKGTFAEATAEAYRYADLLAKENGKYTADLEDGGYTINIRFAGKKV
                     DEKPEEKEQVTIKENIYFEDGTVQTATFKGTFAEATAEAYRYADLLSKEHGKYTADLE
                     DGGYTINIRFAGKEEPEETPEKPEVQDGYASYEEAEAAAKEALKNDDVNKSYTIRQGA
                     DGRYYYVLSPVEAEEEKPEAQNGYATYEEAEAAAKKALENDPINKSYSIRQGADGRYY
                     YVLSPVEAETPEKPVEPSEPSTPDVPSNPSNPSTPDVPSTPDVPSNPSTPEVPSNPST
                     PGNEEKPGNEQKPGNEQKPGNEQKPGNEQKPGNEQKPDQPSKPEKEENGKGGVDSPKK
                     KEKAALPKAGSEAEILTLAAASLSSVAGAFISLKKRK"
BASE COUNT     1141 a    412 c    490 g    614 t
ORIGIN      
        1 atagtgctac aataaaggat ggcactgaac taccgaacct aacgcttgtg gacattgtct
       61 tgggaaattt ggacagtgga cgaatcaaga acaccattaa ttaaattggt gaagttcgat
      121 tgttgatcac cctttttggg taaacaataa ctaaggaatg gcaatatatt tgcttggaaa
      181 cgaatttgat ttaaatagca ttgaatgcaa aaaaatttaa aaggaggaga caaattccac
      241 ccttattaga agggaagttt ccattgtcat gatattatga aaattaataa gaaattatta
      301 atggctgcac ttgcaggtgc aattgtagta acaggtggag taggatctta cgcagctgat
      361 gaacctattg atcttgaaaa acttgaagaa aaaagggata aagaaaatgt aggaaattta
      421 ccaaaattcg ataatgaagt taaagatggt tcagaaaatc caatggctaa atatccagat
      481 ttcgatgatg aagccagtac aagatttgaa acagaaaaca atgaatttga agaaaaaaaa
      541 gttgtttctg ataacttttt tgatcaatca gaacatccgt ttgtagaaaa taaagaagaa
      601 acaccagaaa caccagaaac tgattcagaa gaagaagtaa caatcaaagc taacctaatc
      661 tttgcaaatg gaagcacaca aactgcagaa ttcaaaggaa catttgaaaa agcaacatca
      721 gaagcttatg cgtatgcaga tactttgaag aaagacaatg gagaatatac tgtagatgtt
      781 gcagataaag gttatacttt aaatattaaa tttgctggaa aagaaaaaac accagaagaa
      841 ccaaaagaag aagttactat taaagcaaac ttaatctatg cagatggaaa aacacaaaca
      901 gcagaattca aaggaacatt tgaagaagca acagcagaag catacagata tgcagatgca
      961 ttaaagaagg acaatggaga atatacagta gacgttgcag ataaaggtta tactttaaat
     1021 attaaatttg ctggaaaaga aaaaacacca gaagaaccaa aagaagaagt tactattaaa
     1081 gcaaacttaa tctatgcaga tggaaaaaca caaacagcag aattcaaagg aacatttgaa
     1141 gaagcaacag cagaagcata cagatatgct gacttattag caaaagaaaa tggtaaatat
     1201 acagtagacg ttgcagataa aggttatact ttaaatatta aatttgctgg aaaagaaaaa
     1261 acaccagaag aaccaaaaga agaagttact attaaagcaa acttaatcta tgcagatgga
     1321 aaaactcaaa cagcagagtt caaaggaaca tttgcagaag caacagcaga agcatacaga
     1381 tacgctgact tattagcaaa agaaaatggt aaatatacag cagacttaga agatggtgga
     1441 tacactatta atattagatt tgcaggtaag aaagttgacg aaaaaccaga agaaaaagaa
     1501 caagtaacaa ttaaagaaaa tatatatttt gaagatggaa cagtacaaac tgcaacattt
     1561 aaaggaacat ttgcagaagc gacagcagaa gcatacagat atgcagattt gttatcaaaa
     1621 gaacatggta aatacacagc agacttggaa gatggtggat acactatcaa cattagattt
     1681 gctggaaaag aagaaccaga agaaacacca gaaaaaccag aagtacaaga cggatatgca
     1741 tcatacgaag aagctgaagc agcagctaaa gaagctttga aaaatgatga tgtaaataaa
     1801 tcatatacta ttagacaagg tgcagatgga agatattact atgtattatc accagtagaa
     1861 gctgaagaag aaaaaccaga agcacaaaat ggatatgcaa catacgaaga agcagaagca
     1921 gcagctaaga aagctttgga aaatgatcca atcaataaat cttacagcat aagacaaggt
     1981 gcagatggaa gatactacta tgtattatca ccagtagaag ctgaaactcc tgaaaaacca
     2041 gtagaaccat cagaaccatc aacaccagat gtaccatcaa acccatcaaa tccatcaaca
     2101 ccagatgttc catcaactcc tgatgtacca tcaaatcctt caactccaga agttccttca
     2161 aacccatcaa ctcctggtaa tgaagaaaaa cctggtaacg aacaaaaacc tggtaacgaa
     2221 caaaaacctg gtaacgaaca aaaacctggt aatgaacaaa aacctggtaa tgaacaaaaa
     2281 ccagaccaac cttcaaaacc agaaaaagaa gaaaatggta agggtggagt agattctcca
     2341 aagaaaaaag aaaaagctgc attaccaaaa gctggtagcg aagctgaaat cttaacatta
     2401 gcagcagctt cattatcaag cgttgcaggt gctttcattt cacttaaaaa acgtaaataa
     2461 ttaatcttag ataaaaaata gattaatcta aaaaaatggg acttttataa gtcccatttt
     2521 tgattgcaat gaaactgata caaaaaatgt atcagttttt tcatttacgc ttattttcct
     2581 gtgagtatgt ccaagtttgt cgtataatca tctatcaaaa gcctggcgag tttcgctttt
     2641 tggattttgt cttcgaa
//