From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human glucokinase (GCK) gene, exons 1a-10.
Date: 21 Aug 92 10:11:29 GMT
Distribution: bionet
Checksum: 59882    14

LOCUS       HUMGCK       7807 bp ds-DNA             PRI       21-AUG-1992
DEFINITION  Human glucokinase (GCK) gene, exons 1a-10.
ACCESSION   M93280
KEYWORDS    glucokinase.
SOURCE      Homo sapiens (library: stratagene cat no.946203) placenta DNA.
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 7807)
  AUTHORS   Stoffel,M., Froguel,P., Takeda,J., Zouali,H., Vionnet,N., Nishi,S.,
            Weber,I.T., Harrison,R.W., Pilkis,S.J., Lesage,S., Vaxillaire,M.,
            Velho,G., Sun,F., Iris,F., Passa,P., Cohen,D. and Bell,G.I.
  TITLE     Human glucokinase gene: Isolation, characterization, and
            identification of two missense mutations linked to early-onset
            non-insulin-dependent (type 2) diabetes mellitus
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 89, 7698-7702 (1992)
  STANDARD  full staff_entry
FEATURES             Location/Qualifiers
     mRNA            join(816..1187,1813..1988,2523..2646,3197..3359,
                     3727..3881,4192..4311,4710..4805,4915..5014,5268..5451,
                     5808..5963,6241..6474,6805..7807)
                     /gene="GCK"
     exon            816..1187
                     /number=1a
                     /note="pancreatic beta cell specific"
                     /gene="GCK"
     intron          1188..1812
                     /number=1a
                     /gene="GCK"
     exon            1813..1988
                     /number=1b
                     /note="major liver specific"
                     /gene="GCK"
     intron          1989..2522
                     /number=1b
                     /gene="GCK"
     exon            2523..2646
                     /number=1c
                     /note="'alternative splicing'"
                     /note="minor liver specific"
     intron          2647..3196
                     /number=1c
                     /gene="GCK"
     exon            3197..3359
                     /number=2
                     /gene="GCK"
     intron          3360..3726
                     /number=2
                     /gene="GCK"
     exon            3727..3881
                     /number=3
                     /gene="GCK"
     intron          3882..4191
                     /number=3
                     /gene="GCK"
     exon            4192..4311
                     /number=4
     intron          4312..4709
                     /number=4
                     /gene="GCK"
     exon            4710..4805
                     /number=5
                     /gene="GCK"
     intron          4806..4914
                     /number=5
                     /gene="GCK"
     exon            4915..5014
                     /number=6
                     /gene="GCK"
     intron          5015..5267
                     /number=6
                     /gene="GCK"
     exon            5268..5451
                     /number=7
     intron          5452..5807
                     /number=7
                     /gene="GCK"
     exon            5808..5963
                     /number=8
                     /gene="GCK"
     intron          5964..6240
                     /number=8
                     /gene="GCK"
     exon            6241..6474
                     /number=9
                     /gene="GCK"
     intron          6475..6804
                     /number=9
                     /gene="GCK"
     exon            6805..7807
                     /number=10
                     /gene="GCK"
BASE COUNT     1542 a   2305 c   2311 g   1449 t    200 others
ORIGIN
        1 aggaggagcc agtccctgtg gcctctgttc tgacagtgtc aacctcagcc aggcttgtgg
       61 gccaggtgta ctgttgtggt tcagatttca aggagatagt cagggcaggc cgcgcaaagc
      121 cctccgatgg gctcccctac tgcctggcag acctgtccag ctttggactc tggccctgcg
      181 acctgtccag ctttggactc tggccctgcg acctggaagt caggctgcca agaggtccag
      241 gcagtggctc cactgtggag ggtctctgga gagtttacag ccctagatag gggggttagg
      301 gatgtgagat ggtcccaggg gcctgctcct gagccacgcc aagctgcctg ctccctttcc
      361 tctgcttcca gactcacggg atcctctgct catcagaaca ggagtgtggg agaccctgag
      421 acactgcccc aggatctgaa caggtggcaa aggcttaaca ggctagcgtg cactgtagtg
      481 acaaggcgat tgagtggtca ccatggtgat ggggatggag gctctttgcc accagtccca
      541 gttttatgca tggcagctct aatgacagga tggtcagccc tgctgaggcc actcctggtc
      601 accatgacaa ccacaggccc tctcaggagc acagtaagcc ctggcaggag aatcccccac
      661 tccacacctg gctggagcag gaaatgccga gcggcgcctg agccccaggg aagcaggcta
      721 ggatgtgaga gacacagtca cctgcagcct aattactcaa aagctgtccc caggtcacag
      781 aagggagagg acatttccca ctgaatctgt ctgaaggaca ctaagcccca cagctcaaca
      841 caaccaggag agaaagtgct gaggacgcca cccaagcgcc cagcaatggc cctgcctgga
      901 gaacatccag gctcagtgag gaagggtcca gaagggaatg cttgccgact cgttggagaa
      961 caatgaaaag gagaaactgt gactgaacct caaaccccaa accagcccga ggagaaccac
     1021 attctcccag ggacccaggg cgggcctgac ccctgcggcg gagaagcctt ggatatttcc
     1081 acttcagaag cctactgggg aaggctgagg ggtcccagct ccccacgctg gctgctgtgc
     1141 agatgctgga cgacagagcc aggatggagg ccgccaagaa ggagaaggta tctcgccctc
     1201 cattgggcat tctgggagtg tttgcttgcc tgtccccaac attccatggt ttgtttgagc
     1261 ctcagaatct gattttatgc acaggctctt tgagaagggt cttgccaggg gtgccttctg
     1321 gggcaggaag gcctactgcc tggcagaccc atccagcttt ggactctggt cctgcgaccc
     1381 ggaagtcagg cgtccaagag gtcnnnnnnn nnnnnnnnnn nnngggatcc ccttgcctcc
     1441 aagagcaagt ccagacctgc accagcgcgg gccaggcccc cttaggaccc cctccctgtc
     1501 caagggcatt tcagtaagtg ttctgtggcc aaggcagcct ggtgactttc tgcccgcaca
     1561 aggctgagga atggaagatg ggtaggctct gcacaccccc tcctgctggg cagcaatccc
     1621 taccccatgt tcacagagtg tggccggctg ccccatggct ctgtccccgt ggccctgtca
     1681 actgttaccc cacatgggcc taccctccct ttctggccct gcctctgacc ccatggcagg
     1741 gggcagagta tttgagcagc cgccagctga gccctttcag tgcagaagcc ctgggctgcc
     1801 agcctcaggc agctctccat ccaagcagcc gttgctgcca caggcgggcc ttacgctcca
     1861 aggctacagc atgtgctagg cctcagcagg caggagcatc tctgcctccc aaagcatcta
     1921 cctcttagcc cctcggagag atggcgatgg atgtcacaag gagccaggcc cagacagcct
     1981 tgactctggt aagggtcaca ccaaagttag ggactttgca ctgggagagc agcacccagg
     2041 gcagggcctt ggttttgcag attaccaaaa ctaaggctgg gggcagggaa ggcgagcagg
     2101 cttggggcac cttggaagga ggcacatggg cttgggggtc ctggctaggg cagctgtgcc
     2161 tcnnnnnnnn nnnnnnnnnn nnctgcagca tctgcccagg gactgccctg gcccttggca
     2221 tttcctcagg gacccacagc tccaccagcc ggcccctccc agtgctggaa tagacagttc
     2281 ctcagtccac atctgccaaa ggcggcacta gaaggcatcc tgcctttttt actgcgttct
     2341 ggaggtgggg tcacaaagca ctgctcactg cataaaaggg acagcatcct gcccctggca
     2401 gccctgcctg accagctccg cctctcccac tgctatccaa cctgtacacc ctggtgacca
     2461 tgtccaggcc agtggcctta aggactgtct ctgtactgat ggctccacat ctacctctcc
     2521 agccagactc tcctctgaac tcgggcctca catggccaac tgctacttgg aacaaatcgc
     2581 cccttggctg gcagatgtgt taacatgccc agaccaagat cccaactccc acaacccaac
     2641 tcccaggtca gatggaacct cttcttccca ggcccttctg ttcctctcct cagcccctcc
     2701 cacctccctt cagaataagt ctagactctt atcgctttca ccaagcctgc gcccagcatc
     2761 cctgcacagg gattgttagg acagcctgac gccctgcttc caccctgccc caagatgccc
     2821 tgctctgcag nnnnnnnnnn nnnnnnnnnn gggacgaagc agggtgctac aggacgctgt
     2881 gccacaggga tatcgtcagg gacagaagct actctgccct ctgctgctca cccctccaac
     2941 acgctgtggg ctgcatttgt tgagtggctg gtaccagact ctgctcttct gactttccag
     3001 ctggttttac ctgtagtaaa gtttgagaag atggtcatcc tgaccccggg gtcagaagac
     3061 agaaggaggc ccatggcgtg tgggggagat gccccgtgag gccctcggtg tgcagatgcc
     3121 tggtgacagc cccaccctga ggtccccagc ctaccccctc cccagcccga ctgctcccat
     3181 ccccctccct gtgcaggtag agcagatcct ggcagagttc cagctgcagg aggaggacct
     3241 gaagaaggtg atgagacgga tgcagaagga gatggaccgc ggcctgaggc tggagaccca
     3301 tgaagaggcc agtgtgaaga tgctgcccac ctacgtgcgc tccaccccag aaggctcagg
     3361 taccacatgg taaccggctc ctcatccaga agcagctgtg ggctcagcct agctgggaga
     3421 agcaccccag gcactcccag actcacagcc agcccgagac agaatctcct ggggagcaat
     3481 gaagtcctcg acttgggcca gttctcaccc ttggctcctc tggtccggcc ctggggcact
     3541 cgggctcacc ctggagctgg caaacctcag gaaaactggc gttttaannn nnnnnnnnnn
     3601 nnnnnnntcc cttgtgcctt ccctcctcct ctttgtaata tccggctcag tcacctgggg
     3661 cccacccagc ccaaggccag cctgtgggtg tccctgaggc tgacacactt ctctctgtgc
     3721 ctttagaagt cggggacttc ctctccctgg acctgggtgg cactaacttc agggtgatgc
     3781 tggtgaaggt gggagaaggt gaggaggggc agtggagcgt gaagaccaaa caccagatgt
     3841 actccatccc cgaggacgcc atgaccggca ctgctgagat ggtgagcagc gcaggggccg
     3901 gggcaggggg caaggcatgc aggatctcag ggcccagcta gtcctgacgg gaggtgccac
     3961 ctgtctacca ggggtgggga gagcgggggc tggaggacca cccagcctca gaggcagctg
     4021 gaggcctggg tgaacaggac tnnnnnnnnn nnnnnnnnnn ngacaggcct ggcattcagt
     4081 ggccaggtgt tgcagtgtcc ctgaggaata gcttggcttg aggccgtggg gagggctgcc
     4141 ggccagcgca cccccccatg ccagatggtc accatggcgt gcatcttcca gctcttcgac
     4201 tacatctctg agtgcatctc cgacttcctg gacaagcatc agatgaaaca caagaagctg
     4261 cccctgggct tcaccttctc ctttcctgtg aggcacgaag acatcgataa ggtgggccgg
     4321 gtggaggggc agaaggcaga tgaggggagg cacaggcacc ccagaggaac tctgccttca
     4381 aatgtagccc ccataccctg tgctcagaag ggagatctgg attcaaattg tggccatgtc
     4441 acctgccact ctaatgctgt ggaaaagaag catcacatta gctaattctg gctgtgcgcc
     4501 ttgtgaggca ccagctatga tcaccccact ccagtggaaa gagcagctgg cagtagggtg
     4561 gggctcaaac tcaggcagcc ggctctgggt catcagccag tcatgtcaca tgcctatgct
     4621 agtcagaatg tgnnnnnnnn nnnnnnnnnn nnccctagca ccctgcctcc agatatgtta
     4681 gcagccacga ggcctatctc tccccacagg gcatccttct caactggacc aagggcatca
     4741 aggcctcagg agcagaaggg aacaatgtcg tggggcttct gcgagacgct atcaaacgga
     4801 gaggggtgag ggggcacctg tacctgccgg gggggctgcc ctgggccacc caccccagca
     4861 ctgcctgcct ttctccttgg cttccagcac tgcagcttct gtgcttcttg gcaggacttt
     4921 gaaatggatg tggtggcaat ggtgaatgac acggtggcca cgatgatctc ctgctactac
     4981 gaagaccatc agtgcgaggt cggcatgatc gtgggtaagg gctccttgca cccctgcccc
     5041 ttccagactg ccgaggctcc ctgtgtacaa caggcttcaa gggccctgtg ggtgagggcc
     5101 aaactactta acaaccggtg atgtcagagc agagcctggt gctacagcct gggtggtctt
     5161 ggggtatcaa gatggaagcn nnnnnnnnnn nnnnnnnnng gcaggaacca ggccctactc
     5221 cggggcagtg cagctctcgc tgacagtccc cccgacctcc accccaggca cgggctgcaa
     5281 tgcctgctac atggaggaga tgcagaatgt ggagctggtg gagggggacg agggccgcat
     5341 gtgcgtcaat accgagtggg gcgccttcgg ggactccggc gagctggacg agttcctgct
     5401 ggagtatgac cgcctggtgg acgagagctc tgcaaacccc ggtcagcagc tgtaaggatg
     5461 cccccctccc ccacaaccca ggccctgggc gctctggtgc agcggcagat gggagccggg
     5521 ccattgcaga taatgggctt gtttttaaac aactctgggg aaaagcaaac tgacaatccg
     5581 ttcgtaagct ccatcccttc tgctcagtca tgacctgccc ctgtgagaga tgaagggtta
     5641 gtcccagttg tgatgtgata agcccagacc tctttccttc cgacaggtga tnnnnnnnnn
     5701 nnnnnnnnnn ncctcagtgg ggagcactgg ggccggctcc cggcttccac ctgcatgagg
     5761 ggccctccct cgtgcctgct gatgtaatgg tcctgcccta tgtccaggta tgagaagctc
     5821 ataggtggca agtacatggg cgagctggtg cggcttgtgc tgctcaggct cgtggacgaa
     5881 aacctgctct tccacgggga ggcctccgag cagctgcgca cacgcggagc cttcgagacg
     5941 cgcttcgtgt cgcaggtgga gaggtgtgcg gaggaggagg gtgggtgcaa agggcagggg
     6001 ctggggtcgc ccgggcactg cagacttggt ctcagggcga cgctgagtcc caggcccggg
     6061 gcgcagggac gggaaactag ggcctggggc gggattccgg gcgtgggnnn nnnnnnnnnn
     6121 nnnnnnngct gggggacggc tggccggggc ccctccctgg agaacgagag gccgccgctg
     6181 gagggggatg gactgtcgga gcgacactca gcgaccgccc tacctcctcc cgccccgcag
     6241 cgacacgggc gaccgcaagc agatctacaa catcctgagc acgctggggc tgcgaccctc
     6301 gaccaccgac tgcgacatcg tgcgccgcgc ctgcgagagc gtgtctacgc gcgctgcgca
     6361 catgtgctcg gcggggctgg cgggcgtcat caaccgcatg cgcgagagcc gcagcgagga
     6421 cgtaatgcgc atcactgtgg gcgtggatgg ctccgtgtac aagctgcacc ccaggtgagc
     6481 ctgccccgct ctctccctgg taaagtgggg cccaaaaagc gcgcgctcca aggttccttg
     6541 cggttcccaa gctccaagat ttcgtagtcc tcttctcgtc ccccttggcc tagatttggg
     6601 ggaagggtcg actgcgtgca gggcgcccgg taatgaatgt ggaggatgag gtgggaggan
     6661 nnnnnnnnnn nnnnnnnnnt agagtcttct cgaccccctt ggcctagatt tgggggaagg
     6721 gtcgactgcg tgcagggcgc ccggtaatga atgtggagga tgaggtggga ggagggacgg
     6781 cagccctgct tctcttctgc ccagcttcaa ggagcggttc catgccagcg tgcgcaggct
     6841 gacgcccagc tgcgagatca ccttcatcga gtcggaggag ggcagtggcc ggggcgcggc
     6901 cctggtctcg gcggtggcct gtaagaaggc ctgtatgctg ggccagtgag agcagtggcc
     6961 gcaagcgcag ggaggatgcc acagccccac agcacccagg ctccatgggg aagtgctccc
     7021 cacacgtgct cgcagcctgg cggggcagga ggcctggcct tgtcaggacc caggccgcct
     7081 gccataccgc tggggaacag agcgggcctc ttccctcagt ttttcggtgg gacagcccca
     7141 gggccctaac gggggtgcgg caggagcagg aacagagact ctggaagccc cccacctttc
     7201 tcgctggaat caatttccca gaagggagtt gctcactcag gactttgatg catttccaca
     7261 ctgtcagagc tgttggcctc gcctgggccc aggctctggg aaggggtgcc ctctggatcc
     7321 tgctgtggcc tcacttccct gggaactcat cctgtgtggg gaggcagctc caacagcttg
     7381 accagaccta gacctgggcc aaaagggcag ccaggggctg ctcatcaccc agtcctggcc
     7441 attttcttgc ctgaggctca agaggcccag ggagcaatgg gagggggctc catggaggag
     7501 gtgtcccaag ctttgaatac ccccagagac cttttctctc ccataccatc actgagtggc
     7561 ttgtgattct gggatggacc ctcgcagcag gtgcaagaga cagagccccc aagcctctgc
     7621 cccaaggggc ccacaaaggg gagaagggcc agccctacat cttcagctcc catagcgctg
     7681 gctcaggaag aaaccccaag cagcattcag cacaccccaa gggacaaccc catcatatga
     7741 catgccaccc tctccatgcc caacctaaga ttgtgtgggt tttttaatta aaaatgttaa
     7801 aagtttt
//