From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Saccharomyces cerevisiae long chain base biosynthesis protein
Date: 7 Jan 93 05:54:27 GMT
Distribution: bionet
Checksum: 18164 6

LOCUS       YSCLCB1      2468 bp ds-DNA             PLN       06-JAN-1993
DEFINITION  Saccharomyces cerevisiae long chain base biosynthesis protein
            (LCB1) gene, complete cds.
ACCESSION   M63674
KEYWORDS    long chain base biosynthesis protein; serine palmitoyltransferase.
SOURCE      Saccharomyces cerevisiae DNA.
  ORGANISM  Saccharomyces cerevisiae
            Eukaryota; Fungi; Eumycota; Hemiascomycetes; Endomycetales;
            Saccharomycetaceae.
REFERENCE   1  (bases 1 to 2468)
  AUTHORS   Buede,R., Rinker-Schaffer,C., Pinto,W.J., Lester,R.L. and
            Dickson,R.C.
  TITLE     Cloning and characterization of LCBl, a Saccharomyces gene required
            for biosynthesis of the long-chain base component of sphingolipids
  JOURNAL   J. Bacteriol. 173, 4325-4332 (1991)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     5'UTR           309..331
                     /gene="LCB1"
     CDS             332..2008
                     /function="required for biosynthesis of the long-chain
                     base component of sphingolipids"
                     /EC_number="2.3.1.50"
                     /gene="LCB1"
                     /product="serine palmitoyltransferase"
                     /codon_start=1
                     /translation="MAHIPEVLPKSIPIPAFIVTTSSYLWYYFNLVLTQIPGGQFIVS
                     YIKKSHHDDPYRTTVEIGLILYGIIYYLSKPQQKKSLQAQKPNLSPQEIDALIEDWEP
                     EPLVDPSATDEQSWRVAKTPVTMEMPIQNHITITRNNLQEKYTNVFNLASNNFLQLSA
                     TEPVKEVVKTTIKNYGVGACGPAGFYGNQDVHYTLEYDLAQFFGTQGSVLYGQDFCAA
                     PSVLPAFTKRGDVIVADDQVSLPVQNALQLSRSTVYYFNHNDMNSLECLLNELTEQEK
                     LEKLPAIPRKFIVTEGIFHNSGDLAPLPELTKLKNKYKFRLFVDETFSIGVLGATGRG
                     LSEHFNMDRATAIDITVGSMATALGSTGGFVLGDSVMCLHQRIGSNAYCFSACLPAYT
                     VTSVSKVLKLMDSNNDAVQTLQKLSKSLHDSFASDDSLRSYVIVTSSPVSPVLHLQLT
                     PAYRSRKFGYTCEQLFETMSALQKKSQTNKFIEPYEEEEKFLQSIVDHALINYNVLIT
                     RNTIVLKQETLPIVPSLKICCNAAMSPEELKNACESVKQSILACCQESNK"
BASE COUNT      711 a    586 c    489 g    682 t
ORIGIN      
        1 cgtatttttt tttttttgag gcgccatgat ttcttacacg gtttcttttt tttttccttc
       61 tttccttctt gcttctctgc taacaaattt ttcactcatt cttttttata ggggcatatt
      121 gctgcggtta actgtagtga acgaaagtaa gattgagaaa atatagtact taagaaaaag
      181 aaaaggaaaa ataaaaaaaa ttcttttcaa catcatcgag tagcacagta taagagcgct
      241 ctaaccttct gcctggcctc caatatacac attttgctcg tgtagggtta tttatccttt
      301 tttcttcctt cccacccaaa aaaaaaaagc aatggcacac atcccagagg ttttacccaa
      361 atcaataccg attccggcat ttattgttac cacctcatcg tacctatggt actacttcaa
      421 tctggtgttg actcaaatcc cgggaggcca attcatcgtt tcgtacatca agaaatcgca
      481 tcatgacgat ccatacagga ccacggttga gatagggctt attttatacg ggatcatcta
      541 ttacttgtcc aagccacaac agaaaaagag tcttcaagca cagaagccca acctatcgcc
      601 ccaggagatt gacgcgctaa ttgaggactg ggagcccgag cctctagtcg acccttctgc
      661 caccgatgag caatcgtgga gggtggccaa aacacccgtc accatggaaa tgcccattca
      721 gaaccatatt actatcacca gaaacaacct gcaggagaag tataccaatg ttttcaattt
      781 ggcctcgaac aactttttgc aattgtccgc tacggagccc gtgaaagaag tggtcaagac
      841 cactatcaag aattacggtg tgggcgcctg tggtcccgcc gggttctacg gtaaccagga
      901 cgttcattac acgttggaat atgatttagc acagttcttt ggcacccaag gttccgttct
      961 gtacgggcaa gacttttgtg ccgcaccctc tgttctgcct gctttcacaa agcgtggtga
     1021 tgttatcgtg gcagacgacc aggtgtcatt accagtgcaa aatgctctgc aactaagcag
     1081 atccacagtc tactacttca accacaacga tatgaattcg ctagaatgtt tattaaacga
     1141 gttgaccgaa caggagaaac ttgagaaact gcccgccatt ccaagaaaat ttatcgtcac
     1201 tgagggtatt ttccacaact cgggcgattt agctccgttg cctgagttga ctaagctgaa
     1261 gaacaagtac aagttcagac tatttgttga cgaaaccttc tccattggtg ttcttggcgc
     1321 tacgggccgt gggttgtcag agcacttcaa catggatcgc gcaactgcca ttgacattac
     1381 cgttgggtcc atggccaccg cgttggggtc caccggtggt tttgtcctgg gtgacagtgt
     1441 tatgtgtttg caccagcgta ttggttccaa tgcatattgt ttttctgcct gtttgccggc
     1501 ttacaccgtc acatccgtct ccaaagtctt gaaattgatg gactccaaca acgacgccgt
     1561 ccagacgctg caaaaactat ccaaatcttt gcatgattcc tttgcatctg acgactcctt
     1621 gcgttcatac gtaatcgtca cgtcctctcc agtgtctcct gtcctacatc tgcaactgac
     1681 tcccgcatat aggtctcgca agttcggata cacctgcgaa cagctattcg aaaccatgtc
     1741 agctttgcaa aagaagtccc agacaaacaa attcattgag ccatacgaag aggaggaaaa
     1801 atttctgcag tccatagtag atcatgctct tattaactac aacgttctca tcacaagaaa
     1861 cactattgtt ttaaaacagg agacgctacc aattgtccct agcttgaaaa tctgctgtaa
     1921 cgccgccatg tccccagagg aactcaaaaa tgcttgcgaa agtgtcaagc agtccatcct
     1981 tgcctgttgc caagaatcta ataaataaaa atagaaagcc agtatatgca cacgcacata
     2041 tatatataaa tatttataca ataatacaaa taatcgtaac atcatctctg tcaaattgac
     2101 gtggtgcacg gcgcccagag aatgcgctaa aaattttcgg atccgaaatt ttctttcctt
     2161 ttaccatcga ggcaaagcaa cctgtattat ttatttgttt atttattaat agaaaagaaa
     2221 ggagtacttt cgtggtacgc tttcttgagc attttcggtt tcactaggca gagaactaac
     2281 acaagagaca cagcaaacat caaacaaggt taaaacagca caccaaggca atatgatgca
     2341 ttttagaaag aaatccagta tcagtaacac gagtgatcat gacggagcga accgtgcctc
     2401 agatgtcaag atttctgaag atgacaaggc aagattgaag atgcgtactg cttccgttgc
     2461 tgatccta
//