From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: N. sylvestris tufA gene for chloroplast elongation factor TuA
Date: 12 Jan 93 05:51:16 GMT
Distribution: bionet
Checksum: 16428 4

LOCUS       TOBTUFA      1390 bp ss-mRNA            PLN       12-JAN-1993
DEFINITION  N. sylvestris tufA gene for chloroplast elongation factor TuA
            (EF-TuA), partial sequence.
ACCESSION   D11375
KEYWORDS    EF-TuA; G-binding protein;
            nuclear-encoded chloroplast elongation factor TuA.
SOURCE      Nicotiana sylvestris young leaf cDNA to mRNA.
  ORGANISM  Nicotiana sylvestris
            Eukaryota; Plantae; Embryobionta; Magnoliophyta; Magnoliopsida;
            Asteridae; Solanales; Solanaceae.
REFERENCE   1  (bases 1 to 1390)
  AUTHORS   Murayama,Y., Matsubayashi,T., Sugita,M. and Sugiura,M.
  TITLE     Purification of tobacco chloroplast EF-Tu and analysis of its cDNAs
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
COMMENT     Submitted (19-Jun-1992) to DDBJ by: Masahiro Sugiura Center for
            Gene Research Nagoya University Chikusa, Nagoya 464-01 Japan Phone:
             052-781-5111 x3449 Fax:    052-782-3849.
FEATURES             Location/Qualifiers
     transit_peptide 20..229
     mat_peptide     230..>1390
                     /product="chloroplast elongation factor TuA (EF-TuA)"
                     /codon_start=1
     CDS             20..>1390
                     /gene="tufA"
                     /product="chloroplast elongation factor TuA (EF-TuA)"
                     /codon_start=1
                     /translation="MASISAATATSSTKLVSSNSTNPLLPSSTKPSKLILSSSFTPNC
                     STLFLHSPATPSSTATHRHRRFTVRAARGKFERKKPHVNIGTIGHVDHGKTTLTAALT
                     MALASMGNSAPKKYDEIDAAPEERARGITINTATVEYETENRHYAHVDCPGHADYVKN
                     MITGAAQMDGAILVCSGADGPMPQTKEHILLAKQVGVPNMVVFLNKQDQVDDEELLQL
                     VELEVRELLSSYEFPGDDIPIISGSALLALEALMANPSIKRGENQWVDKIYELMDAVD
                     SYIPIPVRQTELPFLMAIEDVFSITGRGTVATGRVERGTVRIGDTVDIVGLKDTRSTT
                     VTGVEMFQKILDEAMAGDNVGLLLRGIQKIDIQRGMVLAKPGTITPHTKFEAIVYVLK
                     KEEGGRHSPFFSGYRPQFYMRTTDVTGKVTSITTDKGEESKMVMPGDRVNLVVELIMP
                     VACEQGM"
BASE COUNT      339 a    309 c    340 g    402 t
ORIGIN      
        1 ccaacttcaa tctccggcca tggcttcaat ttcagcagcc accgccacct cctccactaa
       61 gcttgtatca tccaactcca ccaatcctct tcttccttcc tccactaaac cctctaagct
      121 tatcctatcc tcttccttta cccctaattg ttccaccctt ttcctccact cacccgctac
      181 cccttcttct actgccaccc atcgccaccg ccgttttact gtccgcgctg cccgtggcaa
      241 attcgagcgg aaaaaacccc atgtcaacat cggtactatt ggccatgttg accacggaaa
      301 gactactctc accgctgctt taaccatggc tcttgcttct atgggaaact ccgcccctaa
      361 gaaatacgac gaaattgatg ccgccccaga agagcgtgct cgtggtatta ctattaacac
      421 tgctactgtt gagtacgaga ctgagaaccg tcactacgcc cacgtggatt gccctggtca
      481 cgctgattat gtcaagaata tgattaccgg tgctgcccaa atggacggcg ctattcttgt
      541 ttgttcaggt gctgatggtc ccatgccaca gactaaggaa cacattttgc ttgctaagca
      601 agtaggtgtc cccaacatgg ttgtgttctt gaacaaacaa gatcaggttg atgatgagga
      661 gctgcttcag cttgttgagt tggaggtaag agagttattg tcaagttatg agtttcctgg
      721 tgatgatatt cctattattt ctggctctgc tcttttggct ttagaggctt tgatggctaa
      781 tcctagtatt aagagaggtg aaaatcaatg ggttgataag atatatgagt tgatggacgc
      841 tgttgatagt tatattccta ttccagttag gcaaactgaa ttgcctttct tgatggctat
      901 tgaagatgtg ttttccatta ctggtagagg tactgtggcg acggggaggg tagagagagg
      961 gactgttagg attggagaca ctgttgatat tgtaggtttg aaggacacta ggagtactac
     1021 cgtgacgggt gttgagatgt ttcaaaagat tttggatgaa gcaatggctg gagacaatgt
     1081 ggggttgttg ttgagaggta ttcaaaagat tgatattcag agaggaatgg tgttggcaaa
     1141 acccggaaca attacccctc acaccaagtt tgaagcgatt gtgtatgtgt tgaagaagga
     1201 ggagggtggt aggcattccc ccttcttttc agggtacagg cctcagtttt acatgaggac
     1261 tactgatgtg accggaaagg ttacttccat tacgactgat aaaggagagg aatctaagat
     1321 ggtcatgcct ggtgatcgtg tgaacttggt ggttgagctc attatgccgg tggcttgtga
     1381 gcaagggatg
//