HDTenEightyP/NetNews huggingface dataset:
From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Rattus norvegicus carboxypeptidase E (CPE) gene, exon 9.
Date: 31 Dec 92 01:31:30 GMT
Distribution: bionet
Checksum: 14023 3

LOCUS       RATCPE09      722 bp ds-DNA             ROD       16-DEC-1992
DEFINITION  Rattus norvegicus carboxypeptidase E (CPE) gene, exon 9.
ACCESSION   L07281
KEYWORDS    carboxypeptidase E; carboxypeptidase H; enkephalin convertase.
SEGMENT     9 of 9
SOURCE      Rattus norvegicus DNA.
  ORGANISM  Rattus norvegicus
            Eukaryota; Animalia; Chorqata; Vertebrata; Mammalia; Theria;
            Eutheria; Rodentia; Myomorpha; Muridae; Murinae.
REFERENCE   1  (bases 1 to 722)
  AUTHORS   Jung,Y.-K., Kunczt,C.J., Pearson,R.K., Dixon,J.E. and Fricker,L.D.
  TITLE     Structural characterization of the rat carboxypeptidase E gene
  JOURNAL   Mol. Endocrinol. 5, 1257-1268 (1991)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     exon            35..722
     3'UTR           131..722
     intron          order(L07280:131..164,1..34)
                     /number=8
                     /gene="CPE"
     CDS             join(L07273:5463..5769,L07274:32..228,L07275:23..190,
                     L07276:49..166,L07277:40..221,L07278:14..154,
                     L07279:25..125,L07280:10..130,35..133)
                     /EC_number="3.4.17.10"
                     /gene="CPE"
                     /product="carboxypeptidase E"
                     /codon_start=1
                     /translation="MAGRGGRVLLALCAALVAGGWLLAAEAQEPGAPAAGMRRRRRLQ
                     QEDGISFEYHRYPELREALVSVWLQCTAISRIYTVGRSFEGRELLVIELSDNPGVHEP
                     GEPEFKYIGNMHGNEAVGRELLIFLAQYLCNEYQRGNETIVNLIHSTRIHIMPSLNPD
                     GFEKAASQPGELKDWFVGRSNAQGIDLNRNFPDLDRIVYVNEKEGGPNNHLLKNLKKI
                     VDQNSKLAPETKAVIHWIMDIPFVLSANLHGGDLVANYPYDETRSGTAHEYSSCPDDA
                     IFQSLARAYSSFNPVMSDPNRPPCRKNDDDSSFVDGTTNGGAWYSVPGGMQDFNYLSS
                     NCFEITVELSCEKFPPEETLKSYWEDNKNSLINYLEQIHRGVKGFVRDLQGNPIANAT
                     ISVDGIDHDVTSGLRMGITGDCLCLETINLQPQLPATWQSQRKWQFLSALLLGVDFEL
                     ESFSERKEEEKEELMEWWKMMSETLNF"
BASE COUNT      214 a    118 c    167 g    223 t
ORIGIN      
        1 tgtacaagcc acaaatctgt tctgttttcc tcaggtggac tttgagctgg agtctttctc
       61 tgaaaggaag gaggaggaga aggaagaatt gatggagtgg tggaaaatga tgtcagagac
      121 tttgaatttc taagaaaggc tcctaactaa tggctttaat atatccatag actatagtaa
      181 gatgcaacgt ggctctttta ttttaggttg tgtgcagtta atatttaaca tcggtttatt
      241 tttgatcatt taagtagtag ttgctaatca cttaaataca cttggacaga accgtagtgt
      301 ctggatcgac ttcattcttt catgaacatt cgctttaaaa tctaggaaga tcctttaacc
      361 tagttggtga caatgtcaca tgacagatgc catgaaaaag tcaaccgatg tagcttggat
      421 ctgtgagcac tgtgtgtatg gcgagaatca catagttcca tataagttgt ccttagtctc
      481 ttgtgctgat ccactgtata agcatgatcc tgttaatgcg cttctgaggg aaggaaaacg
      541 tacgtgcttt gcaaaggggc tctgagcaga aggaaaacct agttcttgcg tgtactttga
      601 agaacagaac tgtattagtc agcctgttag tgcaacttca gagtttgggg ttttgtcttg
      661 attgcagatt ggcccagaat tgcattctaa atgaataaag gataaaaaaa aaaaaccacg
      721 gc
//
From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: dihydrofolate reductase [Pneumocystis carinii, mRNA, 415 nt]
Date: 21 Dec 92 20:11:52 GMT
Distribution: bionet
Checksum: 56365 2

LOCUS       S112346       415 bp    mRNA            NLM       17-NOV-1992
DEFINITION  dihydrofolate reductase [Pneumocystis carinii, mRNA, 415 nt]
ACCESSION   S44630
KEYWORDS    .
SOURCE      Pneumocystis carinii
  ORGANISM  Pneumocystis carinii
            Unclassified.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Reddy,L.V., Zammit,C., Schuman,P. and Crane,L.R.
  TITLE     Detection of Pneumocystis carinii in a rat model of infection by
            polymerase chain reaction.
  JOURNAL   Molecular and Cellular Probes 6, 137-143 (1992)
  STANDARD  full automatic
COMMENT     This entry incorporates information provided by the National
            Library of Medicine's GenInfo Backbone as NCBI gibbsq 112346.
            This sequence comes from Fig. 1.  The authors begin their numbering
            at 139.
FEATURES             Location/Qualifiers
     misc_feature    1..415
                     /partial
                     /note="This feature applies to a gene which lacks a coding
                     region feature. Description: dihydrofolate reductase"
BASE COUNT      135 a     54 c     81 g    145 t
ORIGIN      
        1 gattcatttg aatcgatgaa tgttgtattg atgggtcgaa aaacatggga aagtattcct
       61 ttgcaatttc ggccccttaa aggtcgtatt aatgttgtta tcactcgaaa tgaatctctg
      121 gatctagggc atgttaaaaa aattaatctg aaattcgatt gacaatatta gaaatggaat
      181 tcattctgca aaatccttgg atcatgcttt ggaattgtta tatcgtacat atggttctga
      241 aagttcggtt caaattaatc gaattttcgt tataggtggt gcacagctat ataaagcagc
      301 tatggatcat cctaaattag atagaattat ggctacaata atatacaagg atattcattg
      361 tgatgtattt tttccactta aatttaggga taaagaatgg tcttctgtat ggaaa
//
From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: N.plumbaginifolia mitochondrial DNA for 26S rRNA
Date: 21 Dec 92 20:23:41 GMT
Distribution: bionet
Checksum: 42907 2

LOCUS       TOBMT26S      625 bp ds-DNA             ORG       18-NOV-1992
DEFINITION  N.plumbaginifolia mitochondrial DNA for 26S rRNA
ACCESSION   X68710
KEYWORDS    26S ribosomal RNA; mitochondria; peptidyl tranferase loop region;
            ribosomal RNA.
SOURCE      Mitochondrion Nicotiana plumbaginifolia DNA.
  ORGANISM  Mitochondrion Nicotiana plumbaginifolia
            Eukaryota; Plantae; Embryobionta; Magnoliophyta; Magnoliopsida;
            Asteridae; Solanales; Solanaceae.
REFERENCE   1  (bases 1 to 625)
  AUTHORS   Cseplo,A., Eigel,L., Horvath,V.G., Medgyesy,P., Herrmann,R.G. and
            Koop,H.
  TITLE     Subcellular location of lincomycin resistence in Nicotiana mutants
  JOURNAL   Mol. Gen. Genet. (1992) In press
  STANDARD  full automatic
COMMENT     *source: strain=cv. Viviani; *source: haplotype=diploid; *source:
            is_germline=Y;
               From EMBL    entry NP26S;  dated 29-OCT-1992.
FEATURES             Location/Qualifiers
     rRNA            <1..>625
                     /product="26S ribosomal RNA"
BASE COUNT      152 a    143 c    195 g    135 t
ORIGIN      
        1 ccgtgaagat gcggagtacc aacggctaga cggtaagacc ccgtgcacct taactatagc
       61 ttcgcagtga caaccttaat cgaatgtgta ggataggtgg gaggtggtga cacacaacga
      121 ccaatcctga aagaccactc tttcgtctaa ggatgcctaa ccgccgcacc gatcattcgg
      181 ggggaggcgg gacactgcga ggtgggtagt ttatctgggg cggatgctcc taaagagtaa
      241 cggaggtgtg cgaaggtagg ctcaagctaa gattctgctc gtgagcgtaa tggtataagc
      301 ctgcctgact gtgagaccga ctggtcgaac agagacgaaa gtcggccata gtgatccggg
      361 agtcccgtgt ggaagggctc tcgctcaacg gatcaaaggt acgccgggga taacaggctg
      421 atgactccca agagctctta tcgacggagt cgtttggcac ctcgatgtcg actcatcaca
      481 tcctggggtt gaagaaggtc ccaagggttc ggttgttcgc cgattcaagt ggtacgtgag
      541 ttgggtttag aacgtcgtga gacagttcgg ttcctatcta ccgttggtgt taaagggaga
      601 actgcgagga gccaacccta gtacg
//
From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Aeromonas caviae 16S rDNA
Date: 21 Dec 92 20:38:26 GMT
Distribution: bionet
Checksum: 64220 3

LOCUS       AEO16SRDN    1502 bp ds-DNA             BCT       20-NOV-1992
DEFINITION  Aeromonas caviae 16S rDNA
ACCESSION   X60408
KEYWORDS    16S ribosomal DNA; ribosomal DNA.
SOURCE      Aeromonas caviae DNA.
  ORGANISM  Aeromonas caviae
            Prokaryotae; Facultatively anaerobic gram-negative rods;
            Vibrionaceae.
REFERENCE   1  (bases 1 to 1502)
  AUTHORS   Martinez-Murcia,A.J., Benlloch,S. and Collins,M.D.
  TITLE     Phylogenetic interrelationships of members of the genera Aeromonas
            and Plesiomonas as determined by 16S ribosomal DNA sequencing: lack
            of congruence with results of DNA-DNA hybridizations.
  JOURNAL   Int. J. Syst. Bacteriol. 42, 412-421 (1992)
  STANDARD  full automatic
COMMENT     *source: strain=NCIMB 13016 (T);
               From EMBL    entry AC16SRDN;  dated 13-OCT-1992.
BASE COUNT      373 a    340 c    488 g    301 t
ORIGIN      
        1 gagtttgatc atggctcaga ttgaacgctg gcggcaggcc taacacatgc aagtcgagcg
       61 gcagcgggaa agtagcttgc tacttttgcc ggcgagcggc ggacgggtga gtaatgcctg
      121 ggaaattgcc cagtcgaggg ggataacagt tggaaacgac tgctaatacc gcatacgcct
      181 acgggggaaa gcaggggacc ttcgggcctt gcgcgattgg atatgcccag gtgggattag
      241 ctagttggtg aggtaatggc tcaccaaggc gacgatccct agctggtctg agaggatgat
      301 cagccacact ggaactgaga cacggtccag actcctacgg gaggcagcag tggggaatat
      361 tgcacaatgg gggaaaccct gatgcagcca tgccgcgtgt gtgaagaagg ccttcgggtt
      421 gtaaagcact ttcagcgagg aggaaaggtc agtagctaat atctgctggc tgtgacgtta
      481 ctcgcagaag aagcaccggc taactccgtg ccagcagccg cggtaatacg gagggtgcaa
      541 gcgttaatcg gaattactgg gcgtaaagcg cacgcaggcg gttggataag ttagatgtga
      601 aagccccggg ctcaacctgg gaattgcatt taaaactgtc cagctagagt cttgtagagg
      661 ggggtagaat tccaggtgta gcggtgaaat gcgtagagat ctggaggaat accggtggcg
      721 aaggcggccc cctggacaaa gactgacgct caggtgcgaa agcgtgggga gcaaacagga
      781 ttagataccc tggtagtcca cgccgtaaac gatgtcgatt tggaggctgt gtccttgaga
      841 cgtggcttcc ggagctaacg cgttaaatcg accgcctggg gagtacggcc gcaaggttaa
      901 aactcaaatg aattgacggg ggcccgcaca agcggtggag catgtggttt aattcgatgc
      961 aacgcgaaga accttacctg gccttgacat gtctggaatc ctgcagagat gcgggagtgc
     1021 cttcgggaat cagaacacag gtgctgcatg gctgtcgtca gctcgtgtcg tgagatgttg
     1081 ggttaagtcc cgcaacgagc gcaacccctg tcctttgttg ccagcacgta atggtgggaa
     1141 ctcaagggag actgccggtg ataaaccgga ggaaggtggg gatgacgtca agtcatcatg
     1201 gcccttacgg ccagggctac acacgtgcta caatggcgcg tacagagggc tgcaagctag
     1261 cgatagtgag cgaatcccaa aaagcgcgtc gtagtccgga ttggagtctg caactcgact
     1321 ccatgaagtc ggaatcgcta gtaatcgcaa atcagaatgt tgcggtgaat acgttcccgg
     1381 gccttgtaca caccgcccgt cacaccatgg gagtgggttg caccagaagt agatagctta
     1441 accttcggga gggcgtttac cacggtgtga ttcatgactg gggtgaagtc gtaacaaggt
     1501 aa
//
From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Influenza virus type A/Ibaraki/1/90 RNA for hemagglutinin.
Date: 29 Dec 92 00:27:09 GMT
Distribution: bionet
Checksum: 57855 2

LOCUS       FLAH3N2C      329 bp ss-RNA             VRL       03-DEC-1992
DEFINITION  Influenza virus type A/Ibaraki/1/90 RNA for hemagglutinin.
ACCESSION   D13583
KEYWORDS    hemagglutinin.
SOURCE      Influenza virus type A (individual_isolate A/Ibaraki/1/90) cDNA to
            genomic RNA.
  ORGANISM  Influenza virus type A
            Viridae; ss-RNA enveloped viruses; Negative strand RNA viruses;
            Orthomyxoviridae; Influenzavirus; Influenza A viruses.
REFERENCE   1  (bases 1 to 329)
  AUTHORS   Okuno,Y., Isegawa,Y., Sasao,F. and Ueda,S.
  TITLE     A common neutralizing epitope conserved between H1 and H2
            hemagglutinin of influenza A virus
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
COMMENT     Submitted (07-NOV-1992) to DDBJ by: Yoshinobu Okuno Department of
            Preventive Medicine Research Institute for Microbial Diseases Osaka
            University 3-1 Yamadaoka, Suita Osaka 565 Japan Phone:  06-877-5121
            x3127 Fax:    06-875-1170.
BASE COUNT      125 a     61 c     80 g     63 t
ORIGIN      gene segment 4.
        1 atgacaaacc ctttcaaaat ataaacagga tcacatatgg ggcatgtccc agatatgtta
       61 agcaaaacac tctgaaattg gcaacaggga tgcggaatgt accagagaaa caaactagag
      121 gcatattcgg cgcaatcgca ggtttcatag aaaatggttg ggagggaatg gtagacggtt
      181 ggtacggttt caggcatcaa aattctgagg gcacaggaca agcagcagat cttaaaagca
      241 ctcaagcagc aatcgaccaa atcaacggga aactgaatag gttaatcgag aagacgaacg
      301 agaaattcca tcaaatcgaa aaggaattc
//
From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: D.novaezealandiae mitochondrial gene for 12S ribosomal RNA
Date: 22 Dec 92 22:42:29 GMT
Distribution: bionet
Checksum: 29842 2

LOCUS       DNAMT12RRN    385 bp ds-DNA             ORG       23-NOV-1992
DEFINITION  D.novaezealandiae mitochondrial gene for 12S ribosomal RNA
ACCESSION   X67632
KEYWORDS    12S ribosomal RNA.
SOURCE      Mitochondrion Dinornis novaezealandiae DNA.
  ORGANISM  Mitochondrion Dinornis novaezealandiae
            Eukaryota; Animalia; Chordata; Vertebrata; Aves; Neornithes;
            Eoaves; Ratitae; Dinornithiformes; Dinornithidae.
REFERENCE   1  (bases 1 to 385)
  AUTHORS   Cooper,A., Mourer-Chauvire,C., Chambers,G.K., von Haeseler,A.,
            Wilson,A.C. and Paeaebo,S.
  TITLE     Independent origins of new zealand moas and kiwis
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 89, 8741-8744 (1992)
  STANDARD  full automatic
COMMENT     From EMBL    entry DN12RRNA;  dated 21-AUG-1992.
FEATURES             Location/Qualifiers
     rRNA            <1..>385
                     /product="12S ribosomal RNA"
                     /gene="12S rRNA"
BASE COUNT      124 a    108 c     79 g     74 t
ORIGIN      
        1 gcttggccct aaatctagat acttacccta cacaagtatc cgcctgagaa ctacgagcac
       61 aaacgcttaa aactctaagg acttggcggt gccccaaacc cacctagagg agcctgttct
      121 ataatcgata atccacgata cacccgacca tctcttgccc atgcagccta cataccgccg
      181 tccccagccc gcctaatgaa agaatagcga gcacaacagc tatctcgcta acaagacagg
      241 tcaaggtata gcatatgaga tggaagaaat gggctacatt ttctaacata gaacacctac
      301 gaaagagaag gtgaaaccct cctcagaagg cggatttagc agtaaaatag gacaagaacg
      361 cctactttaa gcctggccct ggggc
//
From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Rattus norvegicus tumor suppressor (p53) gene, exon 10.
Date: 29 Dec 92 20:58:16 GMT
Distribution: bionet
Checksum: 30185 3

LOCUS       RATP53TS8     536 bp ds-DNA             ROD       11-DEC-1992
DEFINITION  Rattus norvegicus tumor suppressor (p53) gene, exon 10.
ACCESSION   L07910 L07781
KEYWORDS    tumor suppressor.
SEGMENT     8 of 8
SOURCE      Rattus norvegicus (strain Sprague-Dawley) DNA.
  ORGANISM  Rattus norvegicus
            Eukaryota; Animalia; Chorqata; Vertebrata; Mammalia; Theria;
            Eutheria; Rodentia; Myomorpha; Muridae; Murinae.
REFERENCE   1  (bases 1 to 536)
  AUTHORS   Hulla,J.E. and Schneider,R.P.Sr..
  TITLE     Structure of the rat p53 tumor suppressor gene
  JOURNAL   Nucleic Acids Res. (1992) In press
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     exon            28..536
                     /number=10
                     /gene="p53"
     intron          order(L07909:134..159,1..27)
                     /number=9
                     /gene="p53"
                     /note="intron 9 is
                     700 bp"
     mRNA            join(L07903:1..174,L07904:41..123,L07905:174..195,
                     L07906:44..316,L07907:34..217,L07907:295..517,
                     L07908:26..162,L07908:241..314,L07909:27..133,28..536)
                     /product="tumor suppressor"
                     /gene="p53"
     CDS             join(L07904:50..123,L07905:174..195,L07906:44..316,
                     L07907:34..217,L07907:295..517,L07908:26..162,
                     L07908:241..314,L07909:27..133,28..109)
                     /gene="p53"
                     /product="tumor suppressor"
                     /codon_start=1
                     /translation="MEDSQSDMSIELPLSQETFSCLWKLLPPDDILPTTATGSPNSME
                     DLFLPQDVAELLEGPEEALQVSAPAAQEPGTEAPAPVAPASATPWPLSSSVPSQKTYQ
                     GNYGFHLGFLQSGTAKSVMCTYSISLNKLFCQLAKTCPVQLWVTSTPPPGTRVRAMAI
                     YKKSQHMTEVVRRWPHHERCSDGDGLAPPQHLIRVEGNPYAEYLDDRQTFRHSVVVPY
                     EPPEVGSDYTTIHYKYMCNSSCMGGMNRRPILTIITLEDSSGNLLGRDSFEVRVCACP
                     GRDRRTEEENFRKKEEHCPELPPGSAKRALPTSTSSSPQQKKKPLDGEYFTLKIRGRE
                     RFEMFRELNEALELKDARAAEESGDSRAHSSYPKTKKGQSTSRHKKPMIKKVGPDSD"
BASE COUNT      111 a    170 c     94 g    161 t
ORIGIN      
        1 atccctccct tttctgtatt cctatagcta cccgaagacc aagaagggcc agtctacgtc
       61 ccgccataaa aaaccaatga tcaagaaagt ggggcctgac tcagactgac agcctctgca
      121 tcctgtcccc atcaccagcc tccccgtccc ctcctttctt gccattttat gactttaggg
      181 cttgttatga gagctgacaa gacaatgcta gtcccttcac tgcctttttt taccttgtag
      241 atagtactcg gccccctcta tgcaaactgg ttcctggccc agattgggga atgggttggt
      301 agttgctggg tctctgctgg tccagcgaaa tcctatccgg tcagttgttg gacctggcac
      361 ctacagtgaa atttcacccc accccaccgc ctgtaagatt ctatcttggg ccctcatacg
      421 atctgtatcc tccggaccca tttcctccac tctgcaaagc ctgtctgcat ttatccatcc
      481 cccacccctc tccctctttt tatctctttt tatatatcca atttcttatt ttacaa
//
From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Rat peptidylglycine alpha-amidating monooxygenase (PAM) gene, exon
Date: 29 Dec 92 19:50:44 GMT
Distribution: bionet
Checksum: 44435 3

LOCUS       RATPAM12      387 bp ds-DNA             ROD       08-DEC-1992
DEFINITION  Rat peptidylglycine alpha-amidating monooxygenase (PAM) gene, exon
            12.
ACCESSION   L01678
KEYWORDS    alternative splicing;
            peptidylglycine alpha-amidating monooxygenase.
SEGMENT     12 of 27
SOURCE      Rattus norvegicus (strain Sprague-Dawley) (library: lambda Fix)
            male adult liver DNA.
  ORGANISM  Rattus norvegicus
            Eukaryota; Animalia; Chorqata; Vertebrata; Mammalia; Theria;
            Eutheria; Rodentia; Myomorpha; Muridae; Murinae.
REFERENCE   1  (sites)
  AUTHORS   Stoffers,D.A., Ouafik,L. and Eipper,B.A.
  TITLE     Characterization of novel mRNAs encoding enzymes involved in
            peptide alpha-amidation
  JOURNAL   J. Biol. Chem. 266, 1701-1707 (1991)
  STANDARD  full automatic
REFERENCE   2  (sites)
  AUTHORS   Eipper,B.A., Green,C.B., Campbell,T.A., Stoffers,D.A.,
            Keutmann,H.T., Mains,R.E. and Ouafik,L.
  TITLE     Alternative splicing and endoproteolytic processing generate
            tissue-specific forms of peptidylglycine alpha-amidating
            monooxygenase (PAM)
  JOURNAL   J. Biol. Chem. 267, 4008-4015 (1992)
  STANDARD  full automatic
REFERENCE   3  (bases 1 to 387)
  AUTHORS   Ouafik,L., Stoffers,D.A., Campbell,T.A., Johnson,R.C.,
            Bloomquist,B.T., Mains,R.E. and Eipper,B.A.
  TITLE     The multifunctional peptidylglycine alpha-amidating monooxygenase
            gene: Exon/intron organization of catalytic, processing and routing
            domains
  JOURNAL   Mol. Endocrinol. 6, 1571-1584 (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     variation       184
     exon            186..289
                     /number=12
                     /gene="PAM"
     intron          order(L01677:144..168,1..185)
                     /number=11
                     /gene="PAM"
                     /note="1.3 kb gap"
BASE COUNT       98 a     79 c     85 g    125 t
ORIGIN      
        1 tggggaactg atgaattatg agggaactcg tttgatctaa caattatttt ttaaaggatc
       61 aggggagcta tctgtgtctt tcttcattca ttctacccac aatgctccaa acctcacagt
      121 actccaacat atagttctgg gcaataagaa gtttaattca ttgttgtatt tgtcttttgg
      181 ggcaggcttt ctaccctgtg gaacaccccg ttgatgttac ttttggtgat atactggcag
      241 ccagatgtgt gttcactggt gaagggagga cagaggccac ccacatcggg taagattctg
      301 cacctccctg ttcagttata ccaggttaaa ccatggttgt aggtaattgt tatctctgcg
      361 aacgaattat acactttcag catcatg
//
From: astwood@ccu.umanitoba.ca (James Astwood)
Subject: ESTs - How Big?
Organization: University of Manitoba, Winnipeg, Manitoba, Canada
Distribution: World
Date: Mon, 21 Dec 1992 20:03:36 GMT

I noticed that GenBank release 74.0 has a new sub-division for ESTs.  Are
there any clear definitions or standards for these things?  For
example, how big should the cDNA be?  What's the difference between a
partial cDNA sequence and an EST?  Are all species of interest, or do we
want only human brain cDNA bits?  Was this sub-division created for NIH and
Craig Venter?
                      - Jim 
--------------------------------------------------------------------
James D. Astwood                    E-Mail: Astwood@ccu.UManitoba.CA
Department of Plant Science         Phone:  (204)-474-6064
University of Manitoba              FAX:    (204)-261-5732
Winnipeg, Manitoba, CANADA          Postal:  R3T 2N2
--------------------------------------------------------------------

-- 
James D. Astwood                    E-Mail: Astwood@ccu.UManitoba.CA
Department of Plant Science         Phone:  (204)-474-6064
University of Manitoba              FAX:    (204)-261-5732
Winnipeg, Manitoba, CANADA          Postal:  R3T 2N2
From: shibumi@net.bio.net (Kenton A. Hoover)
Newsgroups: bionet.molbio.genbank
Subject: Re: bionet.molbio.genbank.updates resumes
Date: 3 Jan 93 22:44:07 GMT
Reply-To: shibumi@net.bio.net
Distribution: bionet
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| Kenton A. Hoover                                 |      shibumi@net.bio.net |
| BIOSCI Network Administrator                     |                          |
| BIOSCI/IntelliGenetics, Inc.                     |          +1 415 962 7300 |
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From: stoehr@embl-heidelberg.de
Newsgroups: bionet.molbio.genbank,bionet.molbio.embldatabank
Subject: Re: EMBL <> GenBank
Date: 23 Dec 92 15:06:54 GMT
Organization: EMBL, European Molecular Biology Laboratory

In article <1992Dec23.081643.11004@comp.bioz.unibas.ch>,
doelz@comp.bioz.unibas.ch (Reinhard Doelz) writes:
> 
> It has been brought to our attention by one of our customers that there 
> are currently thousands (!) of these cases. In contrast to the original 
> assumption that Genbank 74 will now be sort of identical to EMBL 33, I 
> can only warn all of you who trusted in this rather than trying it out. 
> 

Firstly, the assumption that GenBank 74 should be 'sort of identical' to
EMBL 33 is false (depending on how identical 'sort of' means). The two
databases are made at different times.
We do not yet have Genbank 74 here: when we do, as for other quarterly
releases, we determine what we are missing and work to include it all in the
EMBL database. I'm surprised at the figure of 10,000, as I believe that when
we made EMBL 33 that all acc#'s from the previous GenBank release were in.
Another current difference is the accession numbers beginning with 'S' (about
3000 according to your figures) which we do not include in EMBL yet - but
that's a different story.

Regards,
Peter Stoehr
EMBL Data Library
Newsgroups: bionet.molbio.genbank
From: toms@fcs260c2.ncifcrf.gov (Tom Schneider)
Subject: Re: Apologies for Disappearing Division
Organization: Frederick Cancer Research and Development Center
Date: Tue, 22 Dec 1992 02:33:22 GMT

In article <Bzn2A6.559@ncifcrf.gov> toms@fcs260c2.ncifcrf.gov (Tom Schneider) writes:

|Right.  You get splattered with garbage.  Fragments.  Irrelevant species.

Please understand that this is NOT an attack on the NLM, nor on you.  My goal
is to get people to understand the magnitude and character of the problem
facing us, and to suggest some possible solutions.  The more people that
understand these problems, the more likely we will find innovative solutions,
and also the more likely people will chose to pitch in and help clean the
mess.  It probably will take a world wide effort by all molecular biologists to
straighten out and to keep straight.  (Principle 4 in the Philosophy:  The
contributing scientists hold primarily responsibility for the quality of their
contributions to the database.)  My main point is that we can't ignore this
problem too much longer.

  Tom "Cassandra-points-at-Sisyphus, Delila weeps" Schneider
  National Cancer Institute
  Laboratory of Mathematical Biology
  Frederick, Maryland  21702-1201
  toms@ncifcrf.gov
Newsgroups: bionet.molbio.genbank