From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: sgm=sisomicin-gentamicin resistance methylase [Micromonospora
Date: 27 Jan 93 05:59:18 GMT
Distribution: bionet
Checksum: 03952 4

LOCUS       S119202      1122 bp    DNA             BCT       19-JAN-1993
DEFINITION  sgm=sisomicin-gentamicin resistance methylase [Micromonospora
            zionensis, Genomic, 1122 nt]
ACCESSION   S49806 M87057
KEYWORDS    .
SOURCE      Micromonospora zionensis
  ORGANISM  Micromonospora zionensis
            Unclassified.
REFERENCE   1  (bases 1 to 1122)
  AUTHORS   Kojic,M., Topisirovic,L. and Vasiljevic,B.
  TITLE     Cloning and characterization of an aminoglycoside resistance
            determinant from Micromonospora zionensis.
  JOURNAL   J. Bacteriol. 174, 7868-7872 (1992)
  STANDARD  full automatic
COMMENT     This entry [NCBI gibbsq 119202] was created by the journal scanning
            component of NCBI/GenBank at the National Library of Medicine.
            This sequence comes from Fig. 3.
FEATURES             Location/Qualifiers
     CDS             215..1039
                     /note="Sgm;  For the protein sequence (NCBI gibbsq
                     119203):  Method: conceptual translation supplied by
                     author.  This sequence comes from Fig. 3."
                     /gene="sgm"
                     /product="sisomicin-gentamicin resistance methylase"
                     /codon_start=1
                     /translation="MTAPAADDRIDEIERAITKSRRYQTVAPATVRRLARAALVAARG
                     DVPDAVKRTKRGLHEIYGAFLPPSPPNYAALLRHLDSAVDAGDDEAVRAALLRAMSVH
                     ISTRERLPHLDEFYRELFRHLPRPNTLRDLACGLNPLAAPWMGLPAETVYIASDIDAR
                     LVGFVDEALTRLNVPHRTNVADLLEDRLDEPADVTLLLKTLPCLETQQRGSGWEVIDI
                     VNSPNIVVTFPTKSLGQRSKGMFQNYSQSFESQARERSCRIQRLEIGNELIYVIQK"
BASE COUNT      207 a    368 c    335 g    212 t
ORIGIN      
        1 tcgacgtgca aaaagcggcc aagacgggct tgcaggacag gccgaccaat atggacgtga
       61 ctgtcgtgtg gtgtgcggcg cgccccctgc ccgctcgtaa tacccctgta acaactcttg
      121 gtagacgaag cagcaactct tggtgtgcga tccggtgggc cggcgagcgg aattacccgg
      181 ttcgccgccc attgatgacc ttttggaggg accgatgacg gcacctgcgg ccgacgaccg
      241 tatcgacgag attgagcggg ccatcaccaa gagcaggcgt taccagacgg tggcgccggc
      301 caccgtgcgc cgcctggccc gcgctgctct cgtcgccgcg cggggtgacg tgcccgacgc
      361 ggtgaagcgc accaagcggg gtctgcacga gatctacggc gccttcctgc cgcccagccc
      421 tcccaactac gcagcgttgc tgcggcacct ggactcggca gtggacgccg gtgacgacga
      481 ggcggttcga gcggccctac ttcgcgctat gtccgtacat atctccaccc gcgagcgatt
      541 gccgcacctc gacgagttct accgggaact cttccggcac ctcccccgac cgaacacgct
      601 gcgtgacctc gcctgtggtc tcaaccccct ggccgcgccc tggatgggcc tgcccgccga
      661 gaccgtctac atcgcctcgg acatcgacgc ccgcctggtc ggcttcgtgg acgaggccct
      721 gacccgactc aatgttccac atcggacgaa cgtggccgac ctgctcgagg accgtcttga
      781 cgagccggcc gacgtcacgc tattgctgaa gacgctgccc tgtctggaga ctcagcaacg
      841 aggatcgggc tgggaagtga ttgacattgt caactcgccg aatatcgtgg taaccttccc
      901 gaccaagtct ctcggtcagc gatcgaaggg gatgtttcag aactattcac agagttttga
      961 gtcccaggcc agagagcggt catgccgtat tcagcgactg gagattggca acgagctgat
     1021 ttacgtcatt cagaaatagc ttttcgccac tcctcgggag aatcggacgg agtcgtgcgt
     1081 gttcttcggc taaccccttt tttccaccac gactacgtcg ac
//