From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Pseudomonas syringae syrD gene, required for syringomycin
Date: 21 Jul 92 10:07:55 GMT
Distribution: bionet
Checksum: 60100     5

LOCUS       PSESYRD      2508 bp ds-DNA             BCT       21-JUL-1992
DEFINITION  Pseudomonas syringae syrD gene, required for syringomycin
            production, complete cds.
ACCESSION   M97223
KEYWORDS    secretion protein; syrD gene.
SOURCE      Pseudomonas syringae (strain B301D, pathovar syringae) DNA.
  ORGANISM  Pseudomonas syringae
            Prokaryotae; Gracilicutes; Scotobacteria; Pseudomonadaceae.
REFERENCE   1  (bases 1 to 2508)
  AUTHORS   Quigley,N.B., Mo,Y.-Y. and Gross,D.C.
  TITLE     SyrD is required for syringomycin production by Pseudomonas
            syringae pv. syringae and is homologous with a family of secretion
            proteins
  JOURNAL   Unpublished (1992)
  STANDARD  full staff_entry
FEATURES             Location/Qualifiers
     TATA_signal     436..441
                     /gene="syrD"
     RBS             448..451
                     /gene="syrD"
     CDS             462..2162
                     /gene="syrD"
                     /codon_start=1
                     /translation="MKTKQEKKARPGSIMRLLWSSHPWLTFFTLLTGLISGVASIAVV
                     NVINQAIHEETFQRQSLFWFVGLSVVALLFRNGASLFPAYASMRIMTRLRIALCRKIL
                     GTPLEEVDRRGAPNVLTLLTSDIPQLNATLLIMPTILVESAVFLFGIAYLAYLSWVVF
                     AITISLMILGVAMYLLFFMGGMKFTHKVRDEFTAFNEYTHALVFGLKELKLNGIRRRW
                     FSRSAIQESSVRVAKYNYIERLWFTAAENVGQLTLSLLVGCLLFAAPMFAVIDAKTMS
                     ASVLAVLYIMGPLVMLVSAMPMLAQGRIACTRLADFGFSINEPHPEPETSDADNVLLL
                     DHKKSWGSIQLKNVHMNYKDPQSSSGFALGPIDLTIHSGELVYIVGGNGCGKSTLAKV
                     FCGLYIPQEGQLLLDGAAVTDDSRGDYRDLFSAVFSDFHLFNRLIGPDEKEHPSTDQA
                     QTYLSTLGLEDKVKIEGLGYSTTTALSYGQQKRLALVCAYLEDRPIYLLDEWAADQDP
                     PFKRFFYEELLPDLKRRGKTILIITHDDQYFQLADRIIKLADGCIVSDVKCAVEGKRA
                     "
BASE COUNT      484 a    762 c    733 g    529 t
ORIGIN
        1 ctgcagctgc gcacccagtg cccggcggtg atcacccatc cattcacggg cgagcgttgt
       61 ttcttcaacc aagtgcagtt gcatcacccg tactgcatgg gcgaggagct gcgagaggac
      121 ctgctcgaca tgttcggccc cgaccgcctg ccacggttgg tcagctatgg cgacggctca
      181 gccatcgagg acccggtaat ggccttgatc ggtgaagcct acgaggcttg cgctgtgcgt
      241 ttcgaatggc gcaagggcga tgtggtgatg cttgacaaca tgcttgccgc ccacgcccgt
      301 gatccttatg aagaacctcg gctgatcgtt gtcgccatgg gcgaaatgac cgctcggggc
      361 gacgtttggc aaccggcctg aatgatcgac atcccgttat caagccagga cttttaacca
      421 ccggcgcccg cgctggctaa tgcattcaag ggcagcagct aatgaaaacc aagcaagaga
      481 agaaggccag accaggctcg atcatgcgtc tgttatggag cagccatccc tggctgacgt
      541 tctttacgct gctgacaggg ctcatcagcg gcgttgcgtc cattgccgtg gtcaatgtga
      601 tcaaccaggc gattcacgag gagacctttc agcgtcagtc gctgttctgg tttgtcggcc
      661 tgagcgtggt ggcgctcctg ttccgcaacg gtgcgtcgct gtttccggct tacgccagca
      721 tgcgcatcat gacccgtctg cgcattgccc tgtgccgcaa gatcctcggc acgccgcttg
      781 aggaagtcga ccgccgcggt gcgccgaatg tgctgaccct gctgaccagc gatattccgc
      841 aactcaacgc cacgctgttg atcatgccga cgatcctggt ggagtcggcg gtgttcctgt
      901 tcggtattgc ctacctggcc tacctgtcct gggtggtgtt tgcgataacc atctcgctga
      961 tgatcctggg cgttgcaatg tacctgctgt ttttcatggg tggcatgaag ttcacccaca
     1021 aggtgcgcga cgaattcacg gccttcaacg aatacacaca cgcgcttgtg ttcggcctca
     1081 aggaactgaa actcaacggt attcgccgcc gctggttcag ccgctcggcg atccaggagt
     1141 cttcggttcg ggtcgcgaag tacaactaca tcgaacggct ctggttcact gccgccgaaa
     1201 acgttggcca gttgaccctg tcgttgctgg tcggctgcct gctgttcgct gcgccgatgt
     1261 tcgctgttat cgacgccaag accatgagcg ccagtgtgct ggcggtgctg tacatcatgg
     1321 gcccgctggt gatgctggtc agcgccatgc cgatgctggc ccagggacgt attgcctgca
     1381 cgcgactggc ggatttcgga ttctccatca acgaaccgca tccggagccg gaaaccagcg
     1441 atgcagacaa tgtgctgctt ctcgatcaca agaagtcctg gggcagtatc cagctcaaga
     1501 acgtacacat gaactacaag gacccgcaga gcagttccgg ttttgccctg ggacctatcg
     1561 acctgacgat ccactccggt gagctggtct acatcgtcgg cggcaacggt tgtggcaaaa
     1621 gcaccctggc caaagtgttc tgcggcctgt acatcccgca agagggtcag ctactgctcg
     1681 acggtgcggc ggtcacggac gactcgcgcg gcgactatcg cgacctgttc tcggcggtgt
     1741 tctctgactt ccatctgttc aaccgattga taggtcctga cgagaaagaa catcccagca
     1801 ccgatcaggc gcagacatac ctctcgactc tgggcctgga agacaaggtc aagatcgagg
     1861 gacttggtta ctcgaccacc acggccttgt catacggcca gcagaaacgc ctggcactgg
     1921 tgtgcgccta cctggaggac cgtccgattt acctgctgga cgagtgggcc gccgaccagg
     1981 acccgccgtt caagcgcttc ttttacgagg aactgctgcc ggacctcaag cgccggggca
     2041 agacgatcct gatcattact cacgacgatc agtacttcca gctggccgac cgcatcatca
     2101 agttggcgga cggttgcatc gtctccgacg tgaagtgcgc ggttgagggc aagcgcgcct
     2161 gatctgaagc gctgttcaac tttccaggga cgatccttca cgacaggcat gaagccggtt
     2221 gctccctggc cacgacctga ctaccggctg ccgccgcctt ttgcggcgga ggccgcccct
     2281 ttgcgagaag aaatgtatgc acttgccctg accgctgccc aacgcaatat ctggctggat
     2341 cagatgaccc aggcgactcg ccgttataca acatcggcgg ccatctggag atcgacggcg
     2401 ccctcgatta cgaactgttc cagcgagccg tggacctgtt gattgcaaaa cacgacgccc
     2461 tgcgcatggt cctgctcgac cagcgtgccg aggagggcgt gcctgcag
//