From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: L.lactis genes for RecA-like functions, transposase and
Date: 11 Sep 92 10:04:54 GMT
Distribution: bionet
Checksum: 09626     9

LOCUS       LACRECA      4278 bp ds-DNA             BCT       11-SEP-1992
DEFINITION  L.lactis genes for RecA-like functions, transposase and
            p-beta-galactosidase
ACCESSION   X60456
KEYWORDS    p-beta-galactosidase; recA gene; transposase.
SOURCE      Lactococcus lactis DNA.
  ORGANISM  Lactococcus lactis
            Prokaryotae; Firmicutes; Firmibacteria; Gram-positive cocci;
            Streptococcaceae.
REFERENCE   1  (bases 1 to 4278)
  AUTHORS   Huang,D.C., Novel,M., Huang,X.F. and Novel,G.
  TITLE     Nonidentity between plasmid and chromosomal copies of ISS1-like
            sequences in Lactococcus lactis subsp. lactis CNRZ270 and their
            possible role in chromosomal integration of plasmid genes
  JOURNAL   Gene 118, 39-46 (1992)
  STANDARD  full automatic
COMMENT     *source: strain=Z270; *source: clone=X214;
            From EMBL    entry LLRECA;  dated 31-AUG-1992.
FEATURES             Location/Qualifiers
     CDS             294..1127
                     /product="p-beta-galactosidase"
                     /gene="pbg"
                     /codon_start=1
                     /translation="MMVSHARAVKLYKDKGYKGEIGVVHALPTKYPYDPENPADVRAA
                     ELEDIIHNKFILDATYLGHYSDKTMEGVNHILAENGGELDLRDEDFQALDAAKDLNDF
                     LGINYYMSDWMQAFDGETEIIHNGKGEKGSSKYQIKGVGRRVAPDYVPRTDWDWIIYP
                     EGLYDQIMRVKNDYPNYKKIYITENGLGYKDEFVDNTVYDDGRIDYVKQHLEVLSDAI
                     ADGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFDTQERYPKKSAHWYKKLAETQVI
                     E"
     CDS             1419..2318
                     /note="orf2"
                     /product="35 kD protein,identicalwith protein X"
                     /codon_start=1
                     /translation="MTIELKNEYLTVQFKTLGGQLTSIKDKDGLEYLWQADPEYWNGQ
                     APILFPICGSLRNDWAIYRPQDRPFFTGLIRRHGFVRKEEFTLEEVNENSVTFSIKPN
                     AEMLDNYLYQFELRVVYTLNGKSIRTEFQVTNLETEKTMPYFIGAHPAFNCPLVEGEK
                     YEDYSLEFSEVESCSIPKSFPETGLLDLQDRTPFLENQKSLDLDYSLFSHDAITLDRL
                     KSRSVTLRSRKSGKGLRVDFDDFPNLILWSTSNKSPFIALEPWSGLSTSLEEGNILED
                     KPQVTKVLPLDTSKKSYDITILN"
     CDS             complement(2534..3061)
                     /product="transposase"
                     /codon_start=1
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQELLYDRGINVCH
                     TTIYRWVQEYSKVLYYLWKKKNRQSFYSWKMDETYIKIKGRGHYLYRAIDADGLTLDI
                     WLRKKRETQAAYAFLKRLHKQFGEPKAIVTDKAPSLGSAFRKLQSVGLYTKTEHRTVK
                     YLNNLIEQDHRPIKR"
     RBS             3069..3076
     -10_signal      complement(3098..3103)
     -35_signal      complement(3121..3126)
     -35_signal      3271..3276
     -10_signal      3297..3302
     RBS             3311..3315
     CDS             3369..3959
                     /note="orf3"
                     /product="23 kD protein"
                     /codon_start=1
                     /translation="MEVYNLRNTKEKILTATEQLIYKKGYTGTSINDILDETATGKGQ
                     FYYYFDSKKEACLAVIDNHVKIWQKHLLNGILSRDESPLANLKEMLDWIYSDHAQKKI
                     YYGCPVGNLVIELSALDEDFRKPLEQLFSDLQKKIAENLSALTGLLVKQNLPAAHAII
                     AQIQGSLLLLKVTQDLNVLESNFDLLKTSFEKVGEK"
BASE COUNT     1384 a    739 c    811 g   1344 t
ORIGIN
        1 aattctacca taaactattt gctgaatgcc acaagcgtca tgttgaacca tttgtgactt
       61 tgcatcattt tgacacacca gaagctcttc attcaaatgg agatttctta aaccgcgaaa
      121 atatagaaca ctttatagat tatgccgctt tctgttttga agagtttcca gaagtaaact
      181 actggacaac tttcaatgaa attggcccaa ttggtgatgg tcaatacttg gttggtaaat
      241 tccctccagg cattaaatat gatcttgcca aagtcttcca atcacaccac aacatgatgg
      301 tctctcatgc acgtgctgta aaattgtata aggataaagg ttataaaggt gaaattggtg
      361 ttgttcatgc tttaccaact aaataccctt atgatccgga aaatccagca gatgttcgtg
      421 ctgctgaact tgaagacatc atccataaca aatttatctt ggatgcaacc tatcttgggc
      481 actattcaga taaaacgatg gaaggtgtca accatatcct agctgagaat ggtggagaac
      541 ttgatcttcg tgatgaagac ttccaagctc ttgacgcagc taaagatttg aatgatttcc
      601 ttggtatcaa ctattacatg agtgattgga tgcaagcttt tgatggtgag actgaaatca
      661 ttcacaatgg taagggtgaa aaaggaagct ctaagtacca aattaagggt gttggtcgtc
      721 gagtagctcc tgactatgtt ccgcgcacag actgggattg gattatttat cctgaaggct
      781 tgtatgacca aatcatgcga gtgaaaaatg attatccgaa ttacaagaag atttacatca
      841 ctgaaaacgg tctcggatac aaagatgagt ttgtagataa tactgtttat gatgatggtc
      901 gtattgatta cgtgaagcaa cacttggaag ttttatcaga cgcgattgcg gatggtgcaa
      961 atgttaaagg ttacttcatt tggtcactta tggacgtttt ctcatggtca aatggttatg
     1021 aaaaacgtta tggattgttc tatgtagact ttgatacgca agaacgctat cctaagaaat
     1081 cagcacattg gtataagaaa ttagcagaaa ctcaagtgat agagtaatat cacctgagaa
     1141 tgtaataatt tgttatctga tttttagatc aggctttgaa aaggtatata ttattaattt
     1201 agaagattgt cctataattc ctgagcttga agctacctca ctacaaaatg tagtaactgg
     1261 tatgtttcac ctggaaaaga aaggatttgc cattctgatt tagcattatc cgaatttgaa
     1321 tgggaagttt tacaattact cgatgtagca atatgtgaga ctgttcactt cgaggtagat
     1381 tatttgcctt tccaataaca aaaagaaagg aattgactgt gactatcgaa ttaaaaaacg
     1441 agtatcttac ggttcagttt aaaacacttg gtggtcaact gacttcgatt aaagacaaag
     1501 atgggttaga atatctctgg caagctgatc cagagtattg gaatggacag gcaccaattc
     1561 tatttcctat ctgtggtagt ttacgtaatg attgggctat ttatagacct caagacagac
     1621 ctttttttac aggtctaatt cgtagacatg gttttgttcg aaaagaagaa tttactcttg
     1681 aagaagttaa tgaaaatagc gtgactttca gtattaaacc aaatgctgag atgcttgata
     1741 attaccttta tcagtttgaa ctaagagttg tttatacctt aaatgggaaa tccattagaa
     1801 ccgagtttca ggtaacgaac ttggaaactg aaaaaacgat gccttacttc attggagccc
     1861 atccagcatt caattgtcct ttagtagaag gcgaaaagta tgaggactat tctcttgaat
     1921 ttagtgaggt tgagtcttgt tcgataccaa agagtttccc tgagacaggg ttattagatt
     1981 tacaagatcg gacgccattt ttggaaaacc aaaagtctct tgatttggat tactcacttt
     2041 tttctcatga cgccattaca cttgaccgac tgaaatctcg cagcgtgaca cttcgctcga
     2101 gaaagtctgg taaaggtctt cgagttgatt ttgatgattt tccaaacttg attctgtggt
     2161 ctacttccaa taaaagtcca ttcattgcct tagaaccttg gagtgggctt tccacttcac
     2221 ttgaagaagg taatatttta gaagataaac cacaggtaac gaaagtttta cctctagata
     2281 cttctaaaaa gtcctatgac attacgatcc ttaattaaaa tctgtggttc tgttgcgaag
     2341 tttgaaaaat caaaaaccga tctctctgaa ctccaaattc ttaggatgtt attcccatta
     2401 ataccttgat ttcagtagac atcgaaaagc cgaagagcgt tccatttctt cggttctttt
     2461 tatatattcc tcgaagggtc tccatgccct taatcgtgga agaggctgta cggagacttt
     2521 gataaaattt atttcatcgt ttaataggtc gatggtcttg ttctattaaa ttgttaagat
     2581 acttcacagt tcggtgctct gtcttagtat ataaacccac actctgtaac tttctaaaag
     2641 cggagccaag agaaggtgct ttatcggtca caattgcttt cggctcacca aactgtttat
     2701 ggagtcgttt taagaaagca taggctgctt gcgtctcccg tttctttcgt aaccagatat
     2761 ctaaggttaa gccgtccgca tcaattgcac gataaagata atgcccacgt cccttaattt
     2821 tgatataggt ttcgtccatt ttccatgaat agaaggattg tctatttttc ttcttccaaa
     2881 gataatagag gactttgctg tactcttgca cccaacgata aatcgtagta tgacaaacat
     2941 ttattccacg atcatataac aattcctgaa cttcacgata gcttagattg taacgcaggt
     3001 agtaaccaac agcgacaata atgacgtctt ttttgaattg tttgccttta aaatgattca
     3061 ttactctgtc ctctctgtct ttttctcaat tttacactaa aatagatttt ttggaaaact
     3121 ttgcaacaga accgggcaca gttttaaatt ttgcttctga ttctatgcaa agaactgaaa
     3181 tctatcaaaa tgataaggtt caacaacaaa atcatttttt atactaggac acagttaatg
     3241 atatgttttg tactattcgg tacaaaatgt ttgtatttca aaaaagaaga tgttaatata
     3301 attatactaa gtggtacaaa acaatgttgt tagatacaat ctaatctaac catttgtttc
     3361 ctaaaaatat ggaggtgtac aacttgagaa atacgaaaga aaagatattg acggctacag
     3421 aacagcttat atataaaaaa ggttatacag ggacttctat taatgatatt ttagatgaaa
     3481 ctgcgacagg caaagggcaa ttctattatt actttgattc aaaaaaagaa gcttgcctag
     3541 cagtcatcga taatcatgtt aaaatttggc aaaaacattt gctaaatgga atcctatcga
     3601 gagatgaaag tccactggca aacttgaaag agatgctgga ttggatttac agtgatcatg
     3661 cgcaaaaaaa aatatactac ggctgtcctg taggtaatct cgttattgag ctttccgccc
     3721 tagatgagga ttttagaaaa cctcttgaac aactattttc agatttacaa aaaaaaattg
     3781 cggaaaatct atcagcttta acaggactgc tggtgaaaca aaatcttcct gcagcacatg
     3841 caattattgc tcaaattcaa ggaagtcttc tactcttgaa agtgactcaa gatttaaatg
     3901 ttctggaatc aaattttgat ttattaaaaa ctagttttga aaaagtggga gaaaaataat
     3961 gaaaaaattg gatatgatag ttatcggacc aggacctgcc ccgcctactg ctgtgatacg
     4021 acgtaaatct ttatgtcagc agttgaatct aaagaaaaag tcaagctttt gcaggccgat
     4081 gcgattcgga gagaatatcc cattgccgat gttcaaaaaa gatggcaaag ttgtcaaaca
     4141 ttcatagacg ttcttggtca ggggatacct taagcaactc attgcagaat tatcataaaa
     4201 attttatatt taaaggagaa caaaatgttc aataacaata atacagcatt cgtcgttaca
     4261 gacccacaag gtcgaatt
//