From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: E. coli 4-(2'-carboxyphenyl)-4-oxybutyric acid synthase (menC)
Date: 28 Dec 92 21:28:18 GMT
Distribution: bionet
Checksum: 04763 4

LOCUS       ECOMENC       970 bp ds-DNA             BCT       01-DEC-1992
DEFINITION  E. coli 4-(2'-carboxyphenyl)-4-oxybutyric acid synthase (menC)
            gene, complete cds.
ACCESSION   L07256
KEYWORDS    4-(2'-carboxyphenyl)-4-oxybutyric acid synthase; OSB synthase;
            menB gene; menC gene; menaquinone; menaquinone synthesis;
            vitamin K2.
SOURCE      Escherichia coli (individual_isolate PL2024, strain K-12) DNA.
  ORGANISM  Escherichia coli
            Prokaryotae; Gram-negative facultatively anaerobic rods;
            Enterobacteriaceae.
REFERENCE   1  (sites)
  AUTHORS   Sharma,V., Suvarna,K., Meganathan,R. and Hudspeth,M.E.
  TITLE     Menaquinone (Vitamin K2) biosynthesis: Nulceotide sequence and
            expression of the menB gene from Escherichia coli
  JOURNAL   J. Bacteriol. 174, 5057-5062 (1992)
  STANDARD  full automatic
REFERENCE   2  (bases 1 to 970)
  AUTHORS   Sharma,V., Meganathan,R. and Hudspeth,M.E.
  TITLE     Menaquinone (vitamin K2) biosynthesis: Cloning, nucleotide
            sequence, and expression of the menC gene from Escherichia coli
  JOURNAL   Unpublished (1993)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     RBS             3..8
                     /standard_name="Shine-Dalgarno sequence"
                     /gene="menC"
                     /citation=[2]
                     /note="putative"
     CDS             <1..14
                     /gene="menB"
                     /citation=[1]
                     /note="nucleotides 1-149 overlap with 1201-1349 of
                     reported menB gene"
                     /codon_start=3
                     /translation="RNP"
     CDS             14..970
                     /function="converts SHCHC to OSB in menaquinone
                     biosynthesis"
                     /standard_name="OSB synthase"
                     /gene="menC"
                     /evidence="EXPERIMENTAL"
                     /citation=[2]
                     /product="4-(2'-carboxyphenyl)-4-oxybutyric acid synthase"
                     /codon_start=1
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETWEEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELTDTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAVNPDYRDRIAFLEEPCKTRDDSRAFARETGIAIAWDESLREPD
                     FAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLARIA
                     AWLTPDTIPGLDTLDLMQAQQVRRWPGSTLPVVEVDALERLL"
BASE COUNT      201 a    248 c    325 g    196 t
ORIGIN      
        1 aacggaatcc gtaatgcgta gcgcgcaggt ataccgctgg cagatcccca tggacgcggg
       61 ggtggttctg cgcgacaggc ggttaaaaac ccgcgacggg ctgtatgttt gcctgcgtga
      121 aggcgagcgc gaagggtggg gggagatctc cccactgccg ggcttcagtc aggaaacctg
      181 ggaagaggcg caaagtgtgc tgcttgcctg ggtaaataac tggctggcag gcgattgcga
      241 gctaccgcag atgccttccg tggcctttgg cgtaagctgt gcattggcag aactgacaga
      301 tacgttgccg caagcagcca actaccgtgc ggcaccgctg tgtaatggcg atccggacga
      361 tctgatcctc aaacttgcag atatgccagg cgagaaagtg gcgaaggtca aagtgggatt
      421 gtacgaagcg gtgcgcgacg gcatggtggt gaatctgttg ctggaggcaa ttccggatct
      481 gcatttgcgt cttgacgcaa atcgcgcctg gacaccgctg aaaggtcagc agtttgccgt
      541 taacccggat tatcgcgacc gcatcgcgtt tctcgaagag ccgtgcaaaa cccgcgatga
      601 ttcgcgagcg tttgcccgtg aaaccggcat tgccattgcc tgggatgaaa gcctgcgcga
      661 gccggatttt gcctttgtgg ctgaagaggg cgtgcgcgcg gtagttatca aacccacgct
      721 cacgggcagt ctggaaaaag tacgcgagca ggtacaggcg gcgcacgcgc tggggctgac
      781 ggcggtgatc agttcttcca ttgaatcgag cttaggctta acgcaactgg cgcggattgc
      841 cgcctggtta acgccggaca ccattccagg gctggacacg ctggatctga tgcaggcgca
      901 gcaggtacgt cgctggccgg gtagcacgct gcctgtcgtg gaagttgatg cactggagcg
      961 gttgttatga
//