From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: pWE15 cosmid cloning vector.
Date: 4 Sep 92 10:07:12 GMT
Distribution: bionet
Checksum: 25262    12

LOCUS       SYNPWE15     8164 bp ds-DNA             SYN       04-SEP-1992
DEFINITION  pWE15 cosmid cloning vector.
ACCESSION   M99569
KEYWORDS    cloning vector.
SOURCE      Cloning vector DNA.
  ORGANISM  Cloning vector
            Artificial sequences; Cloning vehicles.
REFERENCE   1  (sites)
  AUTHORS   Wahl,G.M., Lewis,K., Ruiz,J., Rothenberg,B.E., Zhao,J. and
            Evans,G.A.
  TITLE     Cosmid vectors for rapid genomic walking, restriction mapping and
            gene transfer
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 84, 2160-2164 (1987)
  STANDARD  full staff_review
REFERENCE   2  (sites)
  AUTHORS   Evans,G.A., Lewis,K. and Rothenberg,B.E.
  TITLE     High efficiency vectors for cosmid microcloning and genomic
            analysis
  JOURNAL   Gene 79, 9-20 (1989)
  STANDARD  full staff_review
REFERENCE   3  (sites)
  AUTHORS   Evans,G.A. and Lewis,K.
  TITLE     Physical mapping of complex genomes by cosmid multiplex analysis
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 86, 5030-5034 (1989)
  STANDARD  full staff_review
REFERENCE   4  (bases 1 to 8164)
  AUTHORS   Evans,G.A.
  JOURNAL   Unpublished (1992)
  STANDARD  simple automatic
BASE COUNT     1935 a   2127 c   2067 g   2035 t
ORIGIN
        1 gaattcgcgg ccgcaattaa ccctcactaa aggatcccta tagtgagtcg tattatgcgg
       61 ccgcgaattc tcatgtttga ccgcttatca tcgataagct ttaatgcggt agtttatcac
      121 agttaaattg ctaacgcagt caggcaccgt gtatgaaatc taacaatgcg ctcatcgtca
      181 tcctcggcac cgtcaccctg gatgctgtag gcataggctt ggttatgccg gtactgccgg
      241 gcctcttgcg ggatatcgtc cattccgaca gcatcgccag tcactatggc gtgctgctag
      301 cgctatatgc gttgatgcaa tttctatgcg cacccgttct cggagcactg tccgaccgct
      361 ttggccgccg cccagtcctg ctcgcttcgc tacttggagc cactatcgac tacgcgatca
      421 tggcgaccac acccgtcctg tggatctgcc tcgctggcct gccgcagttc ttcaacctcc
      481 cggcgcagct tttcgttctc aatttcagca tccctttcgg cataccattt tatgacggcg
      541 gcagagtcat aaagcacctc attacccttg ccaccgcctc gcagaacggg cattccctgt
      601 tcctgccagt tctgaatggt acggatactc gcaccgaaaa tgtcagccag ctgctttttg
      661 ttgacttcca ttgttcattc cacggacaaa aacagagaaa ggaaacgaca gaggccaaaa
      721 agctcgcttt cagcacctgt cgtttccttt cttttcagag ggtattttaa ataaaaacat
      781 taagttatga cgaagaagaa cggaaacgcc ttaaaccgga aaattttcat aaatagcgaa
      841 aacccgcgag gtcgccgccc cgtaacaagg cggatcgccg gaaaggaccc gcaaatgata
      901 ataattatca attgcatact atcgacggca ctgctgccag ataacaccac cggggaaaca
      961 ttccatcatg atggccgtgc ggacatagga agccagttca tccatcgctt tcttgtctgc
     1021 tgccatttgc tttgtgacat ccagcgccgc acattcagca gcgtttttca gcgcgttttc
     1081 gatcaacgtt tcaatgttgg tatcaacacc aggtttaact ttgaacttat cggcactgac
     1141 ggttaccttg ttctgcgctg gctcatcacg ctggatacca aggctgatgt tgtagatatt
     1201 ggtcaccggc tgaggtgttt cgattgccgc tgcgtggata gcaccatttg cgatagcggc
     1261 gtccttgatg aatgacactc cattgcgaat aagttcgaag gagacggtgt cacgaatgcg
     1321 ctggtccagc tcgtcgattg ccttttgtgc agcagaggta tcaatctcaa cgccaagcgt
     1381 catcgaagcg caatattgct gctcaccaaa acgcgtattg accaggtgtt caacggcaaa
     1441 tttctgccct tctgatgtca gaaaggtaaa gtgattttct ttctggtatt cagttgctgt
     1501 gtgtctggtt tcagcaaaac caagctcgcg caattcggct gtgccagatt tagaaggcag
     1561 atcaccagac agcaacgcgc cacggaaaaa cagcgcatac agaacatccg tcgccgcgcc
     1621 ggacaacgtg ataattttat gacccatgat ttatttcctt ttagacgtga gcctgtcgca
     1681 cagcaaagcc gccgaaagtt aacggtttgc ccaggctcac aactgaaaga ctttctacgg
     1741 tgtgcgcgtg cgatgcgcgt agaagactga tttatcaacc tgtctttata tcaggattca
     1801 ttacctgact atttgtgggt aaagttcgta gtgcgctgat cgtgcaaaat gattttagtt
     1861 gggaacagtt cgcaactctg tcccataaaa atcagcatat tcccatctat cccatatcca
     1921 gcgcattgac catcgggata ctgaagggag attccatcat ctcttagaaa gatcaccatc
     1981 tcttttgttt caatttgcat atagctacct ggaggattta tgaatacaag gattttcatg
     2041 gactattacc atgagattga ttttccatct ttattcgcga gagcagtgga aagcgatgac
     2101 gatgtgggta ctacattgcg cattcaccta ctttgtgagc gcatggtcga agcatggata
     2161 tgcgcatgct gtgactgcca agatcctcta cgccggacgc atcgtggccg gcatcaccgg
     2221 cgccacaggt gcggttgctg gcgcctatat cgccgacatc accgatgggg aagatcgggc
     2281 tcgccacttc gggctcatga gcgcttgttt cggcgtgggt atggtggcag gccccgtggc
     2341 cgggggactg ttgggcgcca tctccttgca tgcaccattc cttgcggcgg cggtgctcaa
     2401 cggcctcaac ctactactgg gctgcttcct aatgcaggag tcgcataagg gagagcgtcg
     2461 accgatgccc ttgagagcct tcaacccagt cagctccttc cggtgggcgc ggggcatgac
     2521 tatcgtcgcc gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc
     2581 agcgctctgg gtcattttcg gcgaggaccg ctttcgctgg agcgcgacga tgatcggcct
     2641 gtcgcttgcg gtattcggaa tcttgcacgc cctcgctcaa gccttcgtca ctggtcccgc
     2701 caccaaacgt ttcggcgaga agcaggccat tatcgccggc atggcggccg acgcgctggg
     2761 ctacgtcttg ctggcgttcg cgacgcgagg ctggatggcc ttccccatta tgattcttct
     2821 cgcttccggc ggcatcggga tgcccgcgtt gcaggccatg ctgtccaggc aggtagatga
     2881 cgaccatcag ggacagcttc aaggatcgct cgcggctctt accagcctaa cttcgatcat
     2941 tggaccgctg atcgtcacgg cgatttatgc cgcctcggcg agcacatgga acgggttggc
     3001 atggattgta ggcgccgccc tataccttgt ctgcctcccc gcgttgcgtc gcggtgcatg
     3061 gagccgggcc acctcgacct gaatggaagc cggcggcacc tcgctaacgg attcaccact
     3121 ccaagaattg gagccaatca attcttgcgg agaactgtga atgcgcaaac caacccttgg
     3181 cagaacatat ccatcgcgtc cgccatctcc agcagccgca cgcggcgcat ctcgggcagc
     3241 gttgggtcct gcagatccgg ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
     3301 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
     3361 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     3421 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     3481 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
     3541 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
     3601 agcttcacgc tgccgcaagc actcagggcg caagggctgc taaaggaagc ggaacacgta
     3661 gaaagccagt ccgcagaaac ggtgctgacc ccggatgaat gtcagctact gggctatctg
     3721 gacaagggaa aacgcaagcg caaagagaaa gcaggtagct tgcagtgggc ttacatggcg
     3781 atagctagac tgggcggttt tatggacagc aagcgaaccg gaattgccag ctggggcgcc
     3841 ctctggtaag gttgggaagc cctgcaaagt aaactggatg gctttcttgc cgccaaggat
     3901 ctgatggcgc aggggatcaa gatctgatca agagacagga tgaggatcgt ttcgcatgat
     3961 tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta
     4021 tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca
     4081 ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga
     4141 cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga
     4201 cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct
     4261 cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg
     4321 gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga
     4381 gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca
     4441 tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga
     4501 ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg
     4561 cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc
     4621 gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt
     4681 gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga
     4741 gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca
     4801 tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc
     4861 cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac
     4921 cccgggctcg atcccctcgc gagttggttc agctgctgcc tgaggctgga cgacctcgcg
     4981 gagttctacc ggcagtgcaa atccgtcggc atccaggaaa ccagcagcgg ctatccgcgc
     5041 atccatgccc ccgaactgca ggagtgggga ggcacgatgg ccgctttggt ccggatcttt
     5101 gtgaaggaac cttacttctg tggtgtgaca taattggaca aactacctac agagatttaa
     5161 agctctaagg taaatataaa atttttaagt gtataatgtg ttaaactact gattctaatt
     5221 gtttgtgtat tttagattcc aacctatgga actgatgaat gggagcagtg gtggaatgcc
     5281 tttaatgagg aaaacctgtt ttgctcagaa gaaatgccat ctagtgatga tgaggctact
     5341 gctgactctc aacattctac tcctccaaaa aagaagagaa aggtagaaga ccccaaggac
     5401 tttccttcag aattgctaag ttttttgagt catgctgtgt ttagtaatag aactcttgct
     5461 tgctttgcta tttacaccac aaaggaaaaa gctgcactgc tatacaagaa aattatggaa
     5521 aaatattctg taacctttat aagtaggcat aacagttata atcataacat actgtttttt
     5581 cttactccac acaggcatag agtgtctgct attaataact atgctcaaaa attgtgtacc
     5641 tttagctttt taatttgtaa aggggttaat aaggaatatt tgatgtatag tgccttgact
     5701 agagatcata atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc
     5761 acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat
     5821 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     5881 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     5941 gatctgacgg gtgcgcatga tcgtgctcct gtcgttgagg acccggctag gctggcgggg
     6001 ttgccttact ggttagcaga atgaatcacc gatacgcgag cgaacgtgaa gcgactgctg
     6061 ctgcaaaacg tctgcgacct gagcaacaac atgaatggtc ttcggtttcc gtgtttcgta
     6121 aagtctggaa acgcggaagt cagcgctctt ccgcttcctc gctcactgac tcgctgcgct
     6181 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     6241 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagca
     6301 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     6361 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     6421 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     6481 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     6541 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     6601 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     6661 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     6721 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     6781 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     6841 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     6901 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     6961 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     7021 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     7081 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     7141 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     7201 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     7261 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     7321 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     7381 agttaatagt ttgcgcaacg ttgttgccat tgctgcaggc atcgtggtgt cacgctcgtc
     7441 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     7501 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     7561 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     7621 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     7681 tatgcggcga ccgagttgct cttgcccggc gtcaacacgg gataataccg cgccacatag
     7741 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     7801 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     7861 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     7921 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     7981 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     8041 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga
     8101 aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct
     8161 tcaa
//