From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: lux operon: luxC=fatty acid reductase...luxE=fatty acid reductase
Date: 6 Nov 92 18:02:44 GMT
Distribution: bionet
Checksum: 25112 15

LOCUS       S110516      7669 bp    DNA             NLM       21-OCT-1992
DEFINITION  lux operon: luxC=fatty acid reductase...luxE=fatty acid reductase
            complex synthetase {enteric repetitive intergenic consensus
            sequences} [Xenorhabdus luminescens, strain Hw, Genomic, 5 genes,
            7669 nt]
ACCESSION   S41782 M90092
KEYWORDS    .
SOURCE      Xenorhabdus luminescens strain Hw
  ORGANISM  Xenorhabdus luminescens
            Unclassified.
REFERENCE   1  (bases 1 to 7669)
  AUTHORS   Meighen,E.A. and Szittner,R.B.
  TITLE     Multiple repetitive elements and organization of the lux operons of
            luminescent terrestrial bacteria.
  JOURNAL   J. Bacteriol. 174, 5371-5381 (1992)
  STANDARD  full automatic
COMMENT     This entry incorporates information provided by the National
            Library of Medicine's GenInfo Backbone as NCBI gibbsq 110516.
            This sequence comes from Fig. 2.
            Region: lux operon.
FEATURES             Location/Qualifiers
     CDS             1215..2657
                     /note="For the protein sequence (NCBI gibbsq 110518):
                     This sequence comes from Fig. 3."
                     /gene="luxC"
                     /product="fatty acid reductase"
                     /codon_start=1
                     /translation="MNKKISFIINGRVEIFPESDDLVQSINFGDNSVHLPVLNDSQVK
                     NIIDYNENNELQLHNIINFLYTVGQRWKNEEYSRRRTYIRDLKRYMGYSEEMAKLEAN
                     WISMILCSKGGLYDLVKNELGSRHIMDEWLPQDESYIRAFPKGKSVHLLTGNVPLSGV
                     LSILRAILTKNQCIIKTSSTDPFTANALALSFIDVDPHHPVTRSLSVVYWQHQGDISL
                     AKEIMQHADVVVAWGGEDAINWAVKHAPPDIDVMKFGPKKSFCIIDNPVDLVSAATGA
                     AHDVCFYDQQACFSTQNIYYMGSHYEEFKLALIEKLNLYAHILPNTKKDFDEKAAYSL
                     VQKECLFAGLKVEVDVHQRWMVIESNAGVELNQPLGRCVYLHHVDNIEQILPYVRKNK
                     TQTISVFPWEAALKYRDLLALKGAERIVEAGMNNIFRVGGAHDGMRPLQRLVTYISHE
                     RPSHYTAKDVAVEIEQTRFLEEDKFLVFVP"
     CDS             2671..3594
                     /note="For the protein sequence (NCBI gibbsq 110519):
                     This sequence comes from Fig. 3."
                     /gene="luxD"
                     /product="fatty acid reductase complex transferase"
                     /codon_start=1
                     /translation="MENKSRYKTIDHVICVEENRKIHVWETLPKENSPKRKNTLIIAS
                     GFARRMDHFAGLAEYLSQNGFHVIRYDSLHHVGLSSGTIDEFTMSIGKQSLLAVVDWL
                     NTRKINNLGMLASSLSARIAYASLSEINVSFLITAVGVVNLRYTLERALGFDYLSLPI
                     DELPDNLDFEGHKLGAEVFARDCFDSGWEDLTSTINSMMHLDIPFIAFTANNDDWVKQ
                     DEVITLLSSIRSHQCKIYSLLGSSHDLGENLVVLRNFYQSVTKAAIAMDNGCLDIDVD
                     IIEPSFEHLTIAAVNERRMKIEIENQVISLS"
     CDS             3776..4858
                     /note="For the protein sequence (NCBI gibbsq 110522):
                     This sequence comes from Fig. 3."
                     /gene="luxA"
                     /product="luciferase"
                     /codon_start=1
                     /translation="MKFGNFLLTYQPPQFSQTEVMKRLVKLGRISEECGFDTVWLLEH
                     HFTEFGLLGNPYVAAAYLLGATKKLNVGTAAIVLPTAHPVRQLEEVNLLDQMSKGRFR
                     FGICRGLYNKDFRVFGTDMNNSRALMECWYKLIRNGMTEGYMEADNEHIKFHKVKVLP
                     TAYSQGGAPIYVVAESASTTEWAAQHGLPMILSWIINTNEKKAQIELYNEVAQEYGHD
                     IHNIDHCLSYITSVDHDSMKAKEICRNFLGHWYDSYVNATTIFDDSDKTKGYDFNKGQ
                     WRDFVLKGHKNTNRRVDYSYEINPVGTPQECIDIIQTDIDATGISNICCGFEANGTVD
                     EIISSMKLFQSDVMPFLKEKQRSLLY"
     CDS             4873..5847
                     /note="For the protein sequence (NCBI gibbsq 110523):
                     This sequence comes from Fig. 3."
                     /gene="luxB"
                     /product="luciferase"
                     /codon_start=1
                     /translation="MKFGLFFLNFINSTTIQEQSIARMQEITEYVDKLNFEQILVCEN
                     HFSDNGVVGAPLTVSGFLLGLTEKIKIGSLNHVITTHHPVRIAEEACLLDQLSEGRFI
                     LGFSDCERKDEMHFFNRPEQYQQQLFEECYDIINDALTTGYCNPNGDFYNFPKISVNP
                     HAYTQNGPRKYVTATSCHVVEWAAKKGIPLIFKWDDSNEVKHEYAKRYQAIAGEYGVD
                     LAEIDHQLMILVNYSEDSEKAKEETRAFISDYILAMHPNENFEKKLEEIITENSVGDY
                     MECTTAAKLAMEKCGAKGILLSFESMSDFTHQINAIDIVNDNIKKYHM"
     CDS             6160..7272
                     /note="For the protein sequence (NCBI gibbsq 110525):
                     This sequence comes from Fig. 3."
                     /gene="luxE"
                     /product="fatty acid reductase complex synthetase"
                     /codon_start=1
                     /translation="MTSYVDKQEITASSEIDDLIFSSDPLVWSYDEQEKIRKKLVLDA
                     FRHHYKHCQEYRHYCQAHKVDDNITEIDDIPVFPTSVFKFTRLLTSNENEIESWFTSS
                     GTNGLKSQVPRDRLSIERLLGSVSYGMKYIGSWFDHQMELVNLGPDRFNAHNIWFKYV
                     MSLVELLYPTSFTVTEEHIDFVQTLNSLERIKHQGKDICLIGSPYFIYLLCRYMKDKN
                     ISFSGDKSLYIITGGGWKSYEKESLKRNDFNHLLFDTFNLSNINQIRDIFNQVELNTC
                     FFEDEMQRKHVPPWVYARALDPETLKPVPDGMPGLMSYMDASSTSYPAFIVTDDIGII
                     SREYGQYPGVLVEILRRVNTRKQKGCALSLTEAFGS"
BASE COUNT     2462 a   1251 c   1634 g   2322 t
ORIGIN      
        1 gaattctcag actcaaatag aacaggattc taaagactta agagcagctg tagatcgtga
       61 ttttagtacg atagagccaa cattgagaaa ttatggggca acggaagcac aacttgaaga
      121 cgccagagcc aaaatacaca agcttaacca agaacagagg ttatacaaat gacagttaat
      181 acagaggcac taataaacag cctaggcaag tcctaccaag aaatttttga tgaagggcta
      241 attccttata ggaataagcc aagtggttct cctggggtgc ctaatatttg tattgacatg
      301 gtgaaagagg ggattttttt gtcgtttgaa cggaatagta aaatattaaa cgaaattact
      361 ttaagattgc ttagagacga taaagctttg tttatatttc caaatgaatt gccatcaccg
      421 ttgaagcatt ctatggatag gggatgggtt agagaaaatt taggtgatct gattaaatca
      481 ataccaccga gacaaatttt aaaaaggcag tttggttgga aagatctata tcgttttacg
      541 gatgaaatca gtatgcagat ttcttatgat ttacgtgaac aggttaattc agtgactttc
      601 ttgcttacat cagacgtgag ttggtaattt aatatatata cccttcatcc ttcaagttgc
      661 tgctttgttg gctgctttct ctcaccccag tcacatagtt atctatgctc ctggggattc
      721 gttcacttgc cgccgcgctg caacttgaaa tctattgggt atatgctatt ggtaattatg
      781 gaaaattgcc tgatttatat ataacttaac ttgtaaacca gataataatt tacatgaata
      841 ttatcacgta taaaaaaatt gcgattcttt taatttgaaa tagttcaatt taattgaaac
      901 tttttattaa caaatcttgt tgatgtgaaa attttcgttt gctattttaa cagatattgt
      961 taaacggaga aggcagcatg ttgatgattc actcagccag actgacagtt ttaagcggaa
     1021 aattgcagag tatgatcgca ttctgataaa ggttacaggt cactcgcaac cagaatttca
     1081 tctttgtata ttttgttttg ttatttacgt tgcagcaaga caaaaataga agaaacaaat
     1141 atttatacaa cccgtttgca agagggttaa acagcaattt aagttgaaat tgccctatta
     1201 aatggatggc aaatatgaac aaaaaaattt cattcattat taacggtcga gttgaaatat
     1261 ttcctgaaag tgatgattta gtgcaatcca ttaattttgg tgataatagt gttcatttgc
     1321 cagtattgaa tgattctcaa gtaaaaaaca ttattgatta taatgaaaat aatgaattgc
     1381 aattgcataa cattatcaac tttctctata cggtagggca acgatggaaa aatgaagaat
     1441 attcaagacg caggacatat attcgtgatc taaaaagata tatgggatat tcagaagaaa
     1501 tggctaagct agaggccaac tggatatcta tgattttgtg ctctaaaggt ggcctttatg
     1561 atcttgtaaa aaatgaactt ggttctcgcc atattatgga tgaatggcta cctcaggatg
     1621 aaagttatat tagagctttt ccgaaaggaa aatccgtaca tctgttgacg ggtaatgtgc
     1681 cattatctgg tgtgctgtct atattgcgtg caattttaac aaagaatcaa tgcattataa
     1741 aaacctcatc aactgatcct tttaccgcta atgcattagc gctaagtttt atcgatgtgg
     1801 accctcatca tccggtaacg cgttctttgt cagtcgtata ttggcaacat caaggcgata
     1861 tatcactcgc aaaagagatt atgcaacatg cggatgtcgt tgttgcttgg ggaggggaag
     1921 atgcgattaa ttgggctgta aagcatgcac cacccgatat tgacgtgatg aagtttggtc
     1981 ctaaaaagag tttttgtatt attgataacc ctgttgattt agtatccgca gctacagggg
     2041 cggctcatga tgtttgtttt tacgatcagc aagcttgttt ttccacccaa aatatatatt
     2101 acatgggaag tcattatgaa gagtttaagc tagcgttgat agaaaaattg aacttatatg
     2161 cgcatatatt accaaacacc aaaaaagatt ttgatgaaaa ggcggcctat tccttagttc
     2221 aaaaagaatg tttatttgct ggattaaaag tagaggttga tgttcatcag cgctggatgg
     2281 ttattgagtc aaatgcgggt gtagaactaa atcaaccact tggcagatgt gtgtatcttc
     2341 atcacgtcga taatattgag caaatattgc cttatgtgcg aaaaaataaa acgcaaacca
     2401 tatctgtttt tccttgggag gccgcgctta agtatcgaga cttattagca ttaaaaggtg
     2461 cagaaaggat tgtagaagca ggaatgaata atatatttcg ggttggtggt gctcatgatg
     2521 gaatgagacc tttacaacga ttggtgacat atatttccca tgaaagacca tcccactata
     2581 ctgctaaaga tgttgcggtc gaaatagaac agactcgatt cctggaagaa gataagttcc
     2641 tggtatttgt cccataatag gtaaaagaat atggaaaata aatccagata taaaaccatc
     2701 gaccatgtta tttgtgttga agaaaataga aaaattcatg tctgggagac gctgccaaaa
     2761 gaaaatagtc caaagagaaa aaataccctt attattgcgt cgggttttgc ccgcaggatg
     2821 gatcattttg ccggtctggc agagtatttg tcgcagaatg gatttcatgt gatccgctat
     2881 gattctcttc accacgttgg attgagttca gggacaattg atgaatttac aatgtccata
     2941 ggaaaacaga gtttattagc agtggttgat tggttaaata cacgaaaaat aaataacctc
     3001 ggtatgctgg cttcaagctt atctgcgcgg atagcttatg caagtctatc tgaaattaat
     3061 gtctcgtttt taattaccgc agtcggtgtg gttaacttaa gatatactct cgaaagagct
     3121 ttaggatttg attatctcag cttacctatt gatgaattgc cagataattt agattttgaa
     3181 ggtcataaat tgggtgctga ggtttttgcg agagattgct ttgattctgg ctgggaagat
     3241 ttaacttcta caattaatag tatgatgcat cttgatatac cgtttattgc ttttactgca
     3301 aataatgacg attgggtaaa gcaagatgaa gttattacat tactatcaag catccgtagt
     3361 catcaatgta agatatattc tttactagga agctcacatg atttgggtga gaacttagtg
     3421 gtcctgcgca atttttatca atcggttacg aaagccgcta tcgcgatgga taatggttgt
     3481 ctggatattg atgtcgatat tattgagccg tcattcgaac atttaaccat tgcggcagtc
     3541 aatgaacgcc gaatgaaaat tgagattgaa aatcaagtga tttcgctgtc ttaaaaccta
     3601 taccaataga tttcgagttg cagcgcggcg gcaagtgaac gcattcccag gagcatagat
     3661 aactctgtga ctggggtgcg tgaaagcagc caacaaagca gcaacttgaa ggatgaaggg
     3721 tatattggga tagatagtta actctatcac tcaaatagaa atataaggac tctctatgaa
     3781 atttggaaac tttttgctta cataccaacc cccccaattt tctcaaacag aggtaatgaa
     3841 acggttggtt aaattaggtc gcatctctga ggaatgcggt tttgataccg tatggttact
     3901 tgagcatcat ttcacggagt ttggtttgct tggtaaccct tatgtggctg ctgcttattt
     3961 acttggcgca accaagaaat tgaatgtagg gactgcggct attgttctcc ccaccgctca
     4021 tccagttcgc cagcttgaag aggtgaattt gttggatcaa atgtcaaaag gacgatttcg
     4081 atttggtatt tgtcgggggc tttacaataa agattttcgc gtatttggca cagatatgaa
     4141 taacagtcgt gccttaatgg agtgttggta taagttgata cgaaatggaa tgactgaggg
     4201 atatatggaa gctgacaacg aacatattaa gttccataag gtaaaagtgc tgccgacggc
     4261 atatagtcaa ggtggtgcac ctatttatgt cgttgctgaa tccgcttcca cgactgaatg
     4321 ggccgctcaa catggtttac cgatgatttt aagttggatt ataaatacta acgaaaagaa
     4381 agcacaaatt gagctttata acgaggtcgc tcaagaatat ggacacgata ttcataatat
     4441 cgaccattgc ttatcatata taacatcggt agaccatgac tcaatgaaag cgaaagaaat
     4501 ttgccggaat tttctggggc attggtatga ttcctatgtt aatgccacaa ccatttttga
     4561 tgattcagac aaaacaaagg gctatgattt caataaagga caatggcgcg actttgtctt
     4621 aaaaggacat aaaaatacta atcgtcgcgt tgattacagt tacgaaatca atccggtggg
     4681 aaccccgcag gaatgtattg atataattca aacagacatt gacgccacag gaatatcaaa
     4741 tatttgttgt gggtttgaag ctaatggaac agtagatgaa attatctctt ccatgaagct
     4801 cttccagtct gatgtaatgc cgtttcttaa agaaaaacaa cgttcgctat tatattagct
     4861 aaggaaaatg aaatgaaatt tggcttgttc ttccttaact ttatcaattc aacaactatt
     4921 caagagcaaa gtatagctcg catgcaggaa ataacagaat atgtcgacaa attgaatttt
     4981 gagcagattt tggtgtgtga aaatcatttt tcagataatg gtgttgtcgg cgctcctttg
     5041 actgtttctg gttttttact tggcctaaca gaaaaaatta aaattggttc attgaatcat
     5101 gtcattacaa ctcatcatcc tgtccgcata gcggaagaag cgtgcttatt ggatcagtta
     5161 agcgaaggaa gatttatttt aggatttagt gattgcgaga gaaaggatga aatgcatttt
     5221 ttcaatcgcc ctgaacaata ccagcagcaa ttatttgaag aatgctatga cattattaac
     5281 gatgctttaa caacaggcta ttgtaatcca aatggcgatt tttataattt ccccaaaata
     5341 tccgtgaatc cccatgctta tacgcaaaac gggcctcgga aatatgtaac agcaacaagt
     5401 tgtcatgttg ttgagtgggc tgctaaaaaa ggcattcctc taatctttaa gtgggatgat
     5461 tccaatgaag ttaaacatga atatgcgaaa agatatcaag ccatagcagg tgaatatggt
     5521 gttgacctgg cagagataga tcatcagtta atgatattgg ttaactatag tgaagacagt
     5581 gagaaagcta aagaggaaac gcgtgcattt ataagtgatt atattcttgc aatgcaccct
     5641 aatgaaaatt tcgaaaagaa acttgaagaa ataatcacag aaaactccgt cggagattat
     5701 atggaatgta caactgcggc taaattggca atggagaaat gtggtgcaaa aggtatatta
     5761 ttgtcctttg aatcaatgag tgattttaca catcaaataa acgcaattga tattgtcaat
     5821 gataatatta aaaagtatca catgtaatat accctatgga tttcaaggtg catcgcgacg
     5881 gcaagggagc gaatccccgg gagcatatac ccaatagatt tcaagttgca gtgcggcggc
     5941 aagtgaacgc atccccagga gcatagataa ctatgtgact ggggtaagtg aacgcagcca
     6001 acaaagcagc agcttgaaag atgaagggta tagataacga tgtgaccggg gtgcgtgaac
     6061 gcagccaaca aagaggcaac ttgaaagata acgggtataa aagggtatag cagtcactct
     6121 gccatatcct ttaatattag ctgccgaggt aaaacaggta tgacttcata tgttgataaa
     6181 caagaaatca cagcaagttc agaaattgat gatttgattt tttcgagtga tccattagtc
     6241 tggtcttacg acgaacagga aaagattaga aaaaaacttg tgcttgatgc gtttcgtcat
     6301 cactataaac attgtcaaga ataccgtcac tactgtcagg cacataaagt agatgacaat
     6361 attacggaaa ttgatgatat acctgtattc ccaacatcag tgtttaagtt tactcgctta
     6421 ttaacttcta atgagaacga aattgaaagt tggtttacca gtagtggcac aaatggctta
     6481 aaaagtcagg taccacgtga cagactaagt attgagaggc tcttaggctc tgtaagttat
     6541 ggtatgaaat atattggtag ttggttcgat catcaaatgg aattggtcaa cctgggacca
     6601 gatagattta atgctcataa tatttggttt aaatatgtta tgagcttggt agagttatta
     6661 tatcctacgt cattcaccgt aacagaagaa cacatagatt tcgttcagac attaaatagt
     6721 cttgagcgaa taaaacatca agggaaagat atttgtctta ttggttcgcc atactttatt
     6781 tatttgctct gccgttatat gaaagataaa aatatctcat tttctggaga taaaagtctt
     6841 tatattataa cggggggagg ctggaaaagt tacgaaaaag aatctttgaa gcgtaatgat
     6901 ttcaatcatc ttttattcga cactttcaac ctcagtaata ttaaccagat ccgtgatata
     6961 tttaatcaag ttgaactcaa cacttgtttc tttgaggatg aaatgcaacg taaacatgtt
     7021 ccgccgtggg tatatgcgcg agcacttgat cctgaaacat tgaaaccggt acctgatggg
     7081 atgcctggtt tgatgagtta tatggatgca tcatcaacga gttatccggc atttattgtt
     7141 accgatgata tcggaataat tagcagagaa tatggtcaat atcctggtgt attggttgaa
     7201 attttacgtc gcgttaatac gaggaaacaa aaaggttgtg ctttaagctt aactgaagca
     7261 tttggtagtt gatagtttct ttggaaagag gagcagtcaa aggctcattt gttcaatgct
     7321 tttgcgaaac gttttgtcga actctaggcg aaggttctcg actttccccg catcaggggt
     7381 atatacaagt aaaaaagctc agggggtaaa cctgagcttg ggatgttgat ttttaagtat
     7441 gagatacatg ggcggattta aataacggag tcagtttgga aatatcaacg gtcttttctg
     7501 ctttatcgag gctataagtt tcttgcagtt ttaaccacaa ccgcggagag ctgccaagta
     7561 cttgtgacag ttttattgcc atctctggcg tgactgctgc tttacacgat actaaacgtt
     7621 gaaccgtaga gggagcaaca ttcaatgccc gcgctaagtt cacgaattc
//