From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: E.coli acn, cysB and ribA genes
Date: 10 Apr 92 10:04:35 GMT
Distribution: bionet
Checksum: 54796    10

LOCUS       ECOACN       4245 bp ds-DNA             BCT       10-APR-1992
DEFINITION  E.coli acn, cysB and ribA genes
ACCESSION   X60293
KEYWORDS    acn gene; aconitase; cysB gene; cysB regulatory protein; ribA gene;
            riboflavin biosynthesis.
SOURCE      Escherichia coli DNA.
  ORGANISM  Escherichia coli
            Prokaryota; Bacteria; Gram-negative facultatively anaerobic rods;
            Enterobacteriaceae.
REFERENCE   1  (bases 1 to 4245)
  AUTHORS   Prodromou,C., Artymiuk,P.J. and Guest,J.R.
  TITLE     The aconitase of Escherichia coli: Nucleotide sequence of the
            aconitase gene and amino acid sequence similarity with
            mitochondrial aconitases, the iron-responsive-element-binding
            protein and isopropylmalate isomerases
  JOURNAL   Eur. J. Biochem. 204, 599-609 (1992)
  STANDARD  full automatic
COMMENT     *source: strain=K12 W3110; *source: clone_library=Kohara lambda;
            *source: clone=lambda 13F9, 18B6;
            From EMBL    entry ECACN;  dated 30-MAR-1992.
FEATURES             Location/Qualifiers
     -35_signal      <1..>4245
     CDS             <1..232
                     /gene="cysB"
                     /codon_start=2
                     /translation="DPVADPDLVRVDAHDIFSHSTTKIGFRRSTFLRSYMYDFIQRFA
                     PHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     terminator      246..279
                     /gene="cysB"
     -35_signal      291..296
     -10_signal      314..319
     misc_feature    326
                     /note="+1 base"
     -35_signal      422..427
     -35_signal      435..440
     -10_signal      448..453
     -35_signal      457..462
     -10_signal      457..462
     misc_feature    460
                     /note="+1 base"
     misc_feature    470
                     /note="+1 base"
     -10_signal      480..485
     misc_feature    493
                     /note="+1 base"
     misc_feature    857..875
                     /note="FUR site"
     misc_feature    946..964
                     /note="FUR site"
     -35_signal      1154..1159
     -35_signal      1171..1176
     -10_signal      1176..1183
     misc_feature    1191
                     /note="+1 base"
     -10_signal      1194..1199
     misc_feature    1209
                     /note="+1 base"
     RBS             1222..1229
     CDS             1234..3909
                     /EC_number="4.2.1.3"
                     /product="aconitate hydratase"
                     /gene="acn"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSTLELDMNDVEASLAGPKRPQDRVALPDVPKA
                     FAASNELEVNATHKDRQPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGDPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKGIN
                     VTRSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     terminator      3915..3951
                     /gene="acn/ribA"
     terminator      3970..3994
                     /gene="acn/ribA"
     CDS             complement(3973..>4245)
                     /gene="ribA"
                     /codon_start=1
                     /translation="LQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTN
                     NPKKVEILTEAGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
BASE COUNT     1058 a    995 c   1088 g   1104 t
ORIGIN
        1 ggatccggtc gccgatcccg accttgtgcg tgttgatgct cacgatatct tcagccacag
       61 tacaaccaaa attggttttc gccgtagtac tttcttgcgc agttatatgt atgatttcat
      121 tcagcgtttt gcaccgcatt taacgcgtga tgtcgttgat gcggctgtcg cattgcgctc
      181 taatgaagaa attgaggtca tgtttaaaga tataaaactg ccggaaaaat aatttcttgc
      241 gttattttcg gcacctttta tgtagcgaag gtgccggaat atattctctt ttgttactta
      301 tttctatacc gtgcacaata tttttatccc tcatatttat agggtaaatt acctgtaaag
      361 tagtgtctaa ttacccgcct cgcaaactcc agttttcaaa tatctatttc aggtcaaaag
      421 attgaattat tcactggaga cgattcgtaa attcgctgga tttttgacta aagtttcttt
      481 aggatttgtc tcatcgtatg attaattaca ccaatcgttc aacgtcttgc tgataagtga
      541 tggcgattat atgaggttag caatgccttc tggaaatcag gaaccccgcc gcgatcctga
      601 attaaaacgt aaagcctggc tggcggtttt tcttggttct gcacttttct gggtggttgt
      661 cgcactgctg atttggaaag tgtggggata actatggtcg gtcaggagca actggagtcg
      721 tcaccattat gccagcatag tgacaatgaa acagaaacga aacgggaatg ttccgtcgtt
      781 attccagacg actggcaact aacatcgcag cagcaagcct ttatagaact gtttgctgaa
      841 gatgatcagc cgaaacaata attatcatca ttcttattac ccatttttaa tgaattaaag
      901 ggcttttaat acaccgcagc aataacagct tgagttatct caacacaaaa taataaccgt
      961 taagggtgta gcctatgatc aacacaaata tgaaatattg gtcctggatg ggcgcgtttt
     1021 ctctgtcgat gctcttctgg gccgaactcc tctggatcat tactcactga tccttgaccc
     1081 cgctgcggcg gggttgtcat ttgctttgcc acaaggtttc tcctctttta tcaatttggg
     1141 ttgttatcaa atcgttacgc gatgtttgtg ttatctttaa tattcaccct gaagagaatc
     1201 agggcttcgc aaccctgtca ttaaggagga gctatgtcgt caaccctacg agaagccagt
     1261 aaggacacgt tgcaggccaa agataaaact taccactact acagcctgcc gcttgctgct
     1321 aaatcactgg gcgatatcac ccgtctaccc aagtcactca aagttttgct cgaaaacctg
     1381 ctgcgctggc aggatggtaa ctcggttacc gaagaggata tccacgcgct ggcaggatgg
     1441 ctgaaaaatg cccatgctga ccgtgaaatt gcctaccgcc cggcaagggt gctgatgcag
     1501 gactttaccg gcgtacctgc cgttgttgat ctggcggcaa tgcgcgaagc ggttaaacgc
     1561 ctcggcggcg atactgcaaa ggttaacccg ctctcaccgg tcgacctggt cattgaccac
     1621 tcggtgaccg tcgatcgttt tggtgatgat gaggcatttg aagaaaacgt acgcctggaa
     1681 atggagcgca accacgaacg ttatgtgttc ctgaaatggg gaaagcaagc gttcagtcgg
     1741 tttagcgtcg tgccgccagg cacaggcatt tgccatcagg ttaacctcga atatctcggc
     1801 aaagcagtgt ggagtgaatt gcaggacggt gaatggattg cttatccgga tacactcgtt
     1861 ggtactgact cgcacaccac catgatcaac ggccttggcg tgctggggtg gggcgttggt
     1921 gggatcgaag cagaagccgc aatgttaggc cagccggttt ccatgcttat cccggatgta
     1981 gtgggcttca aacttaccgg aaaattacgt gaaggtatta ccgccacaga cctggttctc
     2041 actgttaccc aaatgctgcg caaacatggc gtggtgggga aattcgtcga attttatggt
     2101 gatggtctgg attcactacc gttggcggat cgcgccacca ttgccaatat gtcgccagaa
     2161 tatggtgcca cctgtggctt cttcccaatc gatgctgtaa ccctcgatta catgcgttta
     2221 agcgggcgca gcgaagatca ggtcgagttg gtcgaaaaat atgccaaagc gcagggcatg
     2281 tggcgtaacc cgggcgatga accaattttt accagtacgt tagaactgga tatgaatgac
     2341 gttgaagcga gcctggcagg gcctaaacgc ccacaggatc gcgttgcact gcccgatgta
     2401 ccaaaagcat ttgccgccag taacgaactg gaagtgaatg ccacgcataa agatcgccag
     2461 ccggtcgatt atgttatgaa cggacatcag tatcagttac ctgatggcgc tgtggtcatt
     2521 gctgcgataa cctcgtgcac caacacctct aacccaagtg tgctgatggc cgcaggcttg
     2581 ctggcgaaaa aagccgtaac tctgggcctc aagcggcaac catgggtcaa agcgtcgctg
     2641 gcaccgggtt cgaaagtcgt ttctgattat ctggcaaaag cgaaactgac accgtatctc
     2701 gacgaactgg ggtttaacct tgtgggatac ggttgtacca cctgtattgg taactctggg
     2761 ccgctgcccg atcctatcga aacggcaatc aaaaaaggcg atttaaccgt cggtgcggtg
     2821 ctgtccggca accgtaactt tgaaggccgt atccatccgc tggttaaaac taactggctg
     2881 gcctcgccgc cgctggtggt tgcctatgcg ctggcgggaa atatgaatat caacctggct
     2941 tctgagccta tcggccatga tcgcaaaggc gatccggttt atctgaaaga tatctggcca
     3001 tcggcacaag aaattgcccg tgcggtagaa caagtctcca cagaaatgtt ccgcaaagag
     3061 tacgcagaag tttttgaagg cacagcagag tggaagggaa ttaacgtcac acgatccgat
     3121 acctacggtt ggcaggagga ctcaacctat attcgcttat cgcctttctt tgatgaaatg
     3181 caggcaacac cagcaccagt ggaagatatt cacggtgcgc ggatcctcgc aatgctgggg
     3241 gattcagtca ccactgacca tatctctccg gcgggcagta ttaagcccga cagcccagcg
     3301 ggtcgatatc tacaaggtcg gggtgttgag cgaaaagact ttaactccta cggttcgcgg
     3361 cgtggtaacc atgaagtgat gatgcgcggc accttcgcca atattcgcat ccgtaatgaa
     3421 atggtgcctg gcgttgaagg ggggatgacg cggcatttac ctgacagcga cgtagtctct
     3481 atttatgatg ctgcgatgcg ctataagcag gagcaaacgc cgctggcggt gattgccggg
     3541 aaagagtatg gatcaggctc cagtcgtgac tgggcggcaa aaggtccgcg tctgcttggt
     3601 attcgtgtgg tgattgccga atcgtttgaa cgaattcacc gttcgaattt aattggcatg
     3661 ggcatcctgc cgctggaatt tccgcaaggc gtaacgcgta aaacgttagg gctaaccggg
     3721 gaagagaaga ttgatattgg cgatctgcaa aacctacaac ccggcgcgac ggttccggtg
     3781 acgcttacgc gcgcggatgg tagccaggaa gtcgtaccct gccgttgtcg tatcgacacc
     3841 gcgacggagt tgacctacta ccagaacgac ggcattttgc attatgtcat tcgtaatatg
     3901 ttgaagtaac aactatttgc ttgccggtta ttttgcttcc ggcaagcaaa tgaattacac
     3961 aatgcaagag ggttatttgt tcagcaaatg gcccattttc tcggctttgg tatcgagata
     4021 atgttcgtta ttggggttac gacctacaat caatggtacg cgttcaacaa tattaatccc
     4081 tgcttcggtc agaatttcga cttttttcgg gttattggtt aacaagcgga cttcattgac
     4141 gccaaggagt ttgaacatat cagcgcaaag agtgaagtcg cgctcatcag cggcgaagcc
     4201 taactggtgg ttagcctcta cggtatcgta accttgatcc tgcag
//