From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Wheat ubiquitin activating enzyme (E1) mRNA, complete cds.
Date: 31 Jul 92 10:02:04 GMT
Distribution: bionet
Checksum: 57375     8

LOCUS       WHTE1A       3695 bp ss-mRNA            PLN       31-JUL-1992
DEFINITION  Wheat ubiquitin activating enzyme (E1) mRNA, complete cds.
ACCESSION   M90663
KEYWORDS    ubiquitin activating enzyme.
SOURCE      Triticum aestivum (strain Augusta) seedling whole seedling cDNA to
            mRNA.
  ORGANISM  Triticum aestivum
            Eukaryota; Plantae; Embryobionta; Magnoliophyta; Liliopsida;
            Commelinidae; Cyperales; Poaceae.
REFERENCE   1  (bases 1 to 3695)
  AUTHORS   Hatfield,P.M. and Vierstra,R.D.
  TITLE     Multiple forms of ubiquitin-activating enzyme E1 from wheat:
            Identification of an essential cysteine by in Vitro mutagenesis
  JOURNAL   J. Biol. Chem. 267, 14799-14803 (1992)
  STANDARD  full staff_entry
FEATURES             Location/Qualifiers
     CDS             278..3433
                     /product="ubiquitin activating enyme"
                     /gene="E1"
                     /codon_start=1
                     /translation="MLPRKREIVAGEVEDLQKKTRAGEGEATREEGDAAMAGRGNEID
                     EDLHSRQLAVYGRETMKPLFGSNVLVSGLQGLGAEIAKNLVLAGVKSVTLHDDGNVEL
                     WDLSSNFFLSENDVGQNRAQACVQKLQELNNAVLVSALTGDLTKEHLSKFQAVVFTDI
                     SLDKAIEFDDYCHSHQPPIAFIKSEVRGLFGSVFCDFGPEFTVLDVDGEEPHTGIVAS
                     ISNDNPALVSCVDDERLEFQDGDLVVFSEVHGMTELNDGKPRKVKNARPYSFFLEEDT
                     SSFGAYVRGGIVTQVKPPKVIKFKPLKEAMSEPGEFLMSDFSKFERPPLLHLAFQALD
                     KFRTELSRFPVAGSTDDVQRVIEYAISINDTLGDRKLEEIDKKLLHHFASGSRAVLNP
                     MAAMFGGIVGQEVVKACSGKFHPLYQFFYFDSVESLPVDPLEPGDLKPKNSRYDAQIS
                     VFGSTLQNKLEEAKIFMVGSGALGCEFLKNLALMGISCSQNGNLTVTDDDVIEKSNLS
                     RQFLFRDWNIGQPKSTVAATAAMVINPKLHVEALQNRASPETENVFNDAFWENLDAVV
                     NALDNVTARMYIDSRCVYFQKPLLESGTLGAKCNTQMVIPHLTENYGASRDPPEKQAP
                     MCTVHSFPHNIDHCLTWARSEFEGLLEKTPTEVNAFLSNPTTYISAARTAGDAQARDQ
                     LERVIECLDRDKCETFQDSITWARLKFEDYFSNRVKQLTFTFPEDSMTSSGAPFWSAP
                     KRFPRPVEFSSSDPSQLSFILAAAILRAETFGIPISEWAKTPNKLAAEAVDKVIVPDF
                     QPKQGVKIVTDEKATSLSSASVDDAAVIEELIAKLEEVSKTLPSGFHMNPIQFEKDDD
                     TNFHMDVIAGFANMRARNYSIPEVDKLKAKFIAGRIIPAIATSTAMATGLVCLELYKA
                     LAGGHKVEDYRNTFANLAIPLFSIAEPVPPKTIKHQELSWTVWDRWTVTGNITLRELL
                     EWLKEKGLNAYSISCGTSLLYNSMFPRHKERLDRKVVDVAREVAKMEVPSYRRHLDVV
                     VACEDDDDNDVDIPLVSVYFR"
BASE COUNT      893 a    897 c    955 g    950 t
ORIGIN
        1 gtccctcccc agcccagaaa ccctagaccg tcgctcgcct gctttccttc cccgacgcga
       61 caggcgaatc caaggtgagt tccgccggat ccgccctccc cgcgcccgct tcccagcgct
      121 ccccgcctca ggacgcgccg gccggtgcag gtttcgcggc ggtcgcggcc ctagcacgcg
      181 ctggatctga cgcgcagctc ccggcggccg cgtactactg cctgctcttc gctgcgtcgg
      241 cggccgtcct cgggctcctc ggcgcgtcgc gcgctatatg cttccccgga agcgggagat
      301 cgtcgccggc gaggtcgagg acttgcagaa gaagacccgt gccggggagg gcgaggccac
      361 cagggaggaa ggagacgcag ccatggctgg gagggggaac gagatcgacg aggacctgca
      421 cagccgccag ctcgccgtct atggccgcga gaccatgaag ccgctcttcg gctccaacgt
      481 cctcgtctct ggactccagg gtctcggtgc tgagatcgca aagaaccttg tccttgcggg
      541 tgtcaagtct gtaaccttgc atgatgatgg taatgtggag ctgtgggact tatcaagcaa
      601 cttcttcctc tcggagaatg atgttgggca aaaccgtgct caagcttgtg tacagaagct
      661 acaagagctg aacaacgctg tcctcgtctc tgccttaacc ggtgatttga ccaaggagca
      721 cctttctaaa ttccaggccg ttgtcttcac tgatatcagc ttagataaag caattgagtt
      781 tgatgattac tgccacagcc accagccacc cattgctttc atcaagtctg aagttcgtgg
      841 tctttttggc agtgtgtttt gtgactttgg tcctgaattt actgttttgg atgtggatgg
      901 tgaggaacca catacaggaa ttgttgcatc aatcagcaat gacaatccag cacttgtatc
      961 ttgtgtggac gatgagcgcc tggagttcca ggatggtgat ctagttgttt tctctgaagt
     1021 ccatggaatg actgagctga atgatggaaa accaagaaaa gttaagaatg caaggcctta
     1081 ctctttcttc ttggaagaag acacttcctc atttggcgca tacgttagag gtggtatcgt
     1141 cacacaggtc aagccaccga aggttattaa attcaaaccc ttgaaagagg ccatgtcaga
     1201 gccgggagaa tttctcatga gtgatttctc caaatttgag cggccacctc ttctgcattt
     1261 ggcattccaa gctttggata agtttaggac tgagttgagc agattccctg ttgctgggtc
     1321 cactgatgat gtgcaaaggg tgatagagta tgctattagc attaatgata ctctgggaga
     1381 taggaaactt gaagaaattg acaaaaagct cctgcaccat tttgccagtg gttccagggc
     1441 tgttctgaat cctatggctg cgatgtttgg tggtattgtc ggtcaggagg tagtgaaagc
     1501 ttgctcaggg aaatttcatc cactttatca gttcttctat tttgattctg tcgagtctct
     1561 ccctgttgac cccttggagc ctggtgattt gaagccaaag aacagtagat atgatgctca
     1621 aatcagcgta tttggatcta cgcttcaaaa caaactggag gaagcaaaaa tctttatggt
     1681 cggttctggg gcacttggat gcgagttctt gaagaacctg gcactaatgg gtatttcttg
     1741 cagccagaat ggaaatctga ctgtgacaga tgatgatgtt attgaaaaga gcaatctgag
     1801 tcgccaattt cttttccgtg actggaacat tgggcaacct aagtccacag ttgctgctac
     1861 agcagctatg gtaattaatc ctaaacttca tgtcgaggcc cttcagaaca gagcaagtcc
     1921 tgagactgaa aatgtgttta atgatgcctt ctgggagaac cttgatgctg ttgtcaatgc
     1981 ccttgacaat gtgactgcaa gaatgtacat agactccaga tgtgtatatt tccagaagcc
     2041 acttttggaa tctgggactc tgggtgctaa atgcaataca cagatggtca tccctcacct
     2101 aacagagaac tatggggctt caagggatcc acctgaaaag caggcaccta tgtgcactgt
     2161 acattcattt cctcataaca ttgatcattg cctaacctgg gctaggtctg agtttgaggg
     2221 tttacttgag aagactccca ctgaagtaaa tgctttcctg tcaaatccta ctacatacat
     2281 aagtgcagca agaactgctg gtgatgcaca ggctagagat cagcttgaac gtgttattga
     2341 gtgtcttgac agagacaagt gcgagacgtt ccaggattct attacctggg cccggcttaa
     2401 gtttgaggat tacttctcca accgtgtgaa gcagctgacg ttcactttcc cagaagactc
     2461 gatgaccagc tctggtgctc ctttctggtc tgctccaaag cggttcccac gacctgtgga
     2521 gttctcgtcg agtgatccaa gtcagcttag ctttattttg gctgctgcaa tattgagggc
     2581 agagactttt ggaataccca tatctgagtg ggccaaaacc ccaaacaagc tggctgctga
     2641 agctgttgac aaggtgattg tgccggattt ccaaccaaag cagggggtta agatagttac
     2701 agatgagaag gccactagtc tatcctctgc atctgttgac gacgctgctg tcattgaaga
     2761 gcttattgcc aagttagaag aagtttccaa aacactgcca tcagggttcc acatgaaccc
     2821 aatacagttt gagaaggatg atgacacaaa cttccatatg gatgtgatag ctggttttgc
     2881 caacatgagg gcaaggaact acagcattcc tgaagtggac aagctaaagg ccaagtttat
     2941 agccggcagg atcatcccag ctatcgccac ctccaccgcg atggccactg gccttgtctg
     3001 ccttgagctt tacaaagccc ttgctggtgg acacaaggtc gaagactacc gcaacacgtt
     3061 tgcaaacctt gcgatccctc tcttctccat tgctgaacca gttccaccca agaccatcaa
     3121 gcaccaggag ctgtcgtgga cggtctggga ccggtggact gtgacaggca acatcacgct
     3181 gagggagctc ctggagtggc tcaaggagaa gggcctgaac gcttacagca tatcctgtgg
     3241 cacctctctg ctgtacaact ccatgttccc caggcacaag gaacggcttg acaggaaggt
     3301 tgttgatgtt gccagggagg tggctaagat ggaggtgccc tcttaccggc gtcatctcga
     3361 cgttgtggtg gcctgtgagg atgacgacga caacgatgtc gacatcccac ttgtgtcggt
     3421 ctacttccgc tgaagaagcg ctccttctgc gtttacatga tcttgtggtt ggtcatgctg
     3481 gatcgactga catgtcatga gtcgcattag tgattgtaag ctgagtgggg gcttaagcca
     3541 cattatcgac tgatttttcc tttctaccat cttgatgtcg atcggatcat tgggggctga
     3601 tctcgggttg ttaccagaca taactattca cgagactagg cacaacatca tcttgataat
     3661 tgttgcatga tgcgttcaca actaaaaaaa aaaaa
//