From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Rattus norvegicus peptidylglycine alpha-amidating monooxygenase-5
Date: 22 Dec 92 22:56:56 GMT
Distribution: bionet
Checksum: 64881 6

LOCUS       RATPAM       2096 bp ss-mRNA            ROD       30-NOV-1992
DEFINITION  Rattus norvegicus peptidylglycine alpha-amidating monooxygenase-5
            (PAM) mRNA, complete cds.
ACCESSION   M82845
KEYWORDS    copper binding sites;
            peptidylglycine alpha-amidating monooxygenase-5.
SOURCE      Rattus norvegicus (strain Sprague-Dawley) (library: lambda ZAPII)
            adult anterior pituitary cDNA to mRNA.
  ORGANISM  Rattus norvegicus
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Rodentia; Myomorpha; Muridae; Murinae.
REFERENCE   1  (bases 1 to 2096)
  AUTHORS   Eipper,B.A., Green,C.B., Campbell,T.A., Stoffers,D.A.,
            Keutmann,H.T., Mains,R.E. and Ouafik,L.
  TITLE     Alternative splicing and endoproteolytic processing generate
            tissue-specific forms of peptidylglycine alpha-amidating
            monooxygenase (PAM)
  JOURNAL   J. Biol. Chem. 267, 4008-4015 (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     exon            <1..971
                     /number=1
                     /gene="PAM"
                     /note="homologous to r-PAM1 gene (J04161); putative"
     sig_peptide     52..126
                     /gene="PAM"
                     /codon_start=1
     mat_peptide     127..987
                     /EC_number="EC 1.14.17.3"
                     /product="peptidylglycine alpha-amidating monooxygenase-5"
                     /gene="PAM"
                     /codon_start=1
     exon            972..>2096
                     /number=2
                     /gene="PAM"
                     /note="novel 3' region"
     CDS             52..990
                     /function="biosynthesis of all a-amidated neuroendocrine
                     peptides"
                     /function="peptide post-translational processing enzyme"
                     /EC_number="EC 1.14.17.3"
                     /gene="PAM"
                     /note="contains only the monooxygenase domain of the
                     bifunctional PAM protein"
                     /product="peptidylglycine alpha-amidating monooxygenase-5"
                     /codon_start=1
                     /translation="MAGRARSGLLLLLLGLLALQSSCLAFRSPLSVFKRFKETTRSFS
                     NECLGTIGPVTPLDASDFALDIRMPGVTPKESDTYFCMSMRLPVDEEAFVIDFKPRAS
                     MDTVHHMLLFGCNMPSSTGSYWFCDEGTCTDKANILYAWARNAPPTRLPKGVGFRVGG
                     ETGSKYFVLQVHYGDISAFRDNHKDCSGVSVHLTRVPQPLIAGMYLMMSVDTVIPPGE
                     KVVNADISCQYKMYPMHVFAYRVHTHHLGKVVSGYRVRNGQWTLIGRQNPQLPQAFYP
                     VEHPVDVTFGDILAARCVFTGEGRTEATHIGFKDTF"
BASE COUNT      580 a    434 c    501 g    581 t
ORIGIN      
        1 ctgccatccc gccggcccgg ccactgcccg ctgccctggt cctgcgcgga catggccgga
       61 cgcgcccgca gcggtctgct actgctgctg ctggggctgc tcgccctgca gagcagctgc
      121 ctggccttca gaagcccact ttctgtcttt aagaggttta aagaaactac cagatcattt
      181 tccaatgaat gccttggtac cattggacca gtcacccctc ttgatgcatc agattttgcg
      241 ctggatattc gcatgcctgg ggttacacct aaagagtctg acacatactt ctgcatgtcc
      301 atgcgtctgc ctgtggatga ggaagccttc gtgattgact tcaagcctcg tgccagcatg
      361 gatactgtcc accatatgct gctgtttgga tgcaatatgc cctcgtccac tggaagttac
      421 tggttttgtg atgaaggaac ctgtacagat aaagccaata ttctatatgc ctgggcaagg
      481 aatgctcccc ccacccggct cccgaaaggt gttggattca gagttggagg agaaactgga
      541 agcaaatact tcgtccttca agttcactat ggcgatatca gtgcttttcg agataatcac
      601 aaagactgct ctggcgtgtc cgtacatctc acacgtgtgc cccagccttt aattgcgggc
      661 atgtacctta tgatgtctgt tgacactgtc ataccaccag gagagaaagt agtgaatgct
      721 gacatttcgt gccaatacaa aatgtatcca atgcatgtgt ttgcctacag agtccacact
      781 caccatttag gtaaggtggt gagcggatac agagtaagaa acggacagtg gacactgatt
      841 ggacgccaga acccccagct gccacaggct ttctaccctg tggaacaccc cgttgatgtt
      901 acttttggtg atatactggc agccagatgt gtgttcactg gtgaagggag gacagaggcc
      961 acccatatcg gattcaaaga tactttctga gtgcaattgg aagcttggga gaagatacag
     1021 aaaagtgaag gtgatacaga aaaaaagagt tttcagaaga gtcgtgacta cgaactagga
     1081 accacactgg agaggagaga ggaaggacag gagcatttga tggagttttc atagatgatg
     1141 ggttttcgta gaagatggct ctccgtctta ccttaaacta tcaatttgta tcaaagaaga
     1201 agctttcaag tagcattcct agtgtatttg ttaaattttt ggagaaggct ggagaaaatc
     1261 aataaacgta ttgactttca taaaggaaga taggacgata tatacacaat cacactgcgt
     1321 agaggggaca ggccgtgacc tccgtgacag tctgtgacgc ttggggttcc acggggcaga
     1381 gagtattgat cagctgccct ctgttcggtg ctcttgtgtg gccacaggaa cggtagttaa
     1441 acgccagagg ttattccgtt aaccttttca gaaagtaaag aatgtagttc aaaataaacc
     1501 ctttcagaga gctgcatgta ttgatttaca agctcaagtg ggaggcagta gagtatttta
     1561 accctccagg gccttcaccc tttggataag ggggcatatt acacccttgg ccagactgta
     1621 tctgtggagg cattaacggc ttgttacagt gccagaatga gaaaccggag tggcccccag
     1681 agatttcttc cgggttcatg agtctagaat aattccagcc tttttaaaat tatacatttt
     1741 gctttaaaat gataccctaa aatgaggata ttactctatc ctttcgtaac tctctggtga
     1801 gataaagtaa acactatttt taatacagag gagatagaaa gggattatgg caaattaagt
     1861 tgttggatga gtctgaattt tgaatgttta atttatacta ctgtataaaa cgactatagt
     1921 ttttttccag aagaataata aactaataag gcatgtttga agaaatctaa tggtgaataa
     1981 aatttttatt atgatgcata ttatatatgc atataatgtt taatgtgcct tttaaatttg
     2041 gaaaaaatac tgtctacagt gtttctgtgt tttgtacaat agcaggtgta gtctcc
//