From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: S.marcescens thrA1A2BC genes for aspartokinase, homoserine
Date: 21 Jul 92 10:08:23 GMT
Distribution: bionet
Checksum: 55835    13

LOCUS       SMATHR       6190 bp ds-DNA             BCT       21-JUL-1992
DEFINITION  S.marcescens thrA1A2BC genes for aspartokinase, homoserine
            dehydrogenase, homoserine kinase and threonine synthase
ACCESSION   X60821
KEYWORDS    aspartokinase; homoserine dehydrogenase; homoserine kinase;
            thrA1A2BC operon; threonine synthase.
SOURCE      Serratia marcescens DNA.
  ORGANISM  Serratia marcescens
            Prokaryotae; Gram-negative facultatively anaerobic rods;
            Enterobacteriaceae.
REFERENCE   1  (bases 1 to 6190)
  AUTHORS   Omori,K., Suzuki,S. and Komatsubara,S.
  TITLE     Analysis of the thrA1A2BC genes of Serratia marcescens Sr41
  JOURNAL   Unpublished (1991)
  STANDARD  full automatic
COMMENT     *source: strain=Sr41; *source: clone=pWT201; *source: isolate=8000
            (wild-type);
            From EMBL    entry SMTHR;  dated 12-AUG-1991.
FEATURES             Location/Qualifiers
     -35_signal      736..741
     -10_signal      757..762
     RBS             778..780
     CDS             804..872
                     /note="thrA1A2BC operon attenuator"
                     /evidence=EXPERIMENTAL
                     /codon_start=1
                     /translation="MRNISLTTTIITTTDTTGNGAG"
     repeat_unit     861..870
                     /rpt_type=inverted
                     /note="inverted repeat 1"
     repeat_unit     891..904
                     /rpt_type=inverted
                     /note="inverted repeat 2"
     repeat_unit     896..905
                     /rpt_type=inverted
                     /note="inverted repeat 1"
     repeat_unit     912..925
                     /rpt_type=inverted
                     /note="inverted repeat 2"
     RBS             940..943
     CDS             953..3412
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /product="homoserine dehydrogenase"
                     /gene="thrA1A2"
                     /evidence=EXPERIMENTAL
                     /codon_start=1
                     /translation="MRVLKFGGTSVANAERFLRVADIMESNARQGQVATVLSAPAKIT
                     NHLVAMIDKTVAGQDILPNMSDAERIFADLLSGLAQALPGFEYDRLKGVVDQEFAQLK
                     QVLHGVSLLGQCPDSVNAAIICRGEKLSIAIMEGVFRAKGYPVTVINPVEKLLAQGHY
                     LESTVDIAESTLRIAAAAIPADHIVLMAGFTAGNDKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRTVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTSNPQAPGTLIGKDSTDADMPVKGITNLNNMAMINVSGPGMKGMVGMAAR
                     VFAVMSRAGISVVLITQSSSEYSISFCVPQGELQRARRALEEEFYLELKDGVLDPLDV
                     MERLAIISVVGDGMRTLRGISARFFSALARANINIVAIAQGSSERSISVVVSNDSATT
                     GVRVSHQMLFNTDQVIEVFVIGVGGVGGALIEQIYRQQPWLKQKHIDLRVCGIANSRV
                     MLTNVHGIALDSWRDALAGAQEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYVD
                     FLADGFHVVTPNKKANTSSMNYYQQLRAAAAGSHRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELVRFSGILSGSLSFIFGKLDEGLSLSAATLQARANGYTEPDPRDDLSGMDVARK
                     LLILAREAGYKLELSDIEVEPVLPPSFDASGDVDTFLARLPELDKEFARNVANAAEQG
                     KVLRYVGLIDEGRCKVRIEAVDGNDPLYKVKNGENALAFYSRYYQPLPLVLRGYGAGN
                     DVTAAGVFADLLRTLSWKLGV"
     RBS             3404..3407
                     /evidence=EXPERIMENTAL
     CDS             3414..4343
                     /EC_number="2.7.1.39"
                     /product="homoserine kinase"
                     /gene="thrB"
                     /evidence=EXPERIMENTAL
                     /codon_start=1
                     /translation="MVKVYAPASIGNVSVGFDVLGAAVSPIDGTLLGDCVSVEAAETF
                     SLQNAGRFVSKLPAEPKENIVYQCWERFCQEIGREVPVAMRLEKNMPIGSGLGSSACS
                     VVAGLMAMNEFCDRPLDKTTLLGLMGELEGRISGSVHYDNVAPCYLGGLQLMLEEEGI
                     ISQEVPCFDDWLWVMAYPGIKVSTAEARAILPAQYRRQDCISHGRYLAGFIHACHTRQ
                     PQLAAKLMQDVIAEPYRTRLLPGFAEARKAAQEIGALACGISGSGPTLFAVCNDGATA
                     QRMAAWLQQHYLQNDEGFVHICRLDTAGARLLG"
     RBS             4338..4340
                     /evidence=EXPERIMENTAL
     CDS             4347..5636
                     /EC_number="4.2.99.2"
                     /product="threonine synthase"
                     /gene="thrC"
                     /evidence=EXPERIMENTAL
                     /codon_start=1
                     /translation="MKLYNLKDHNEQVSFAQAIKQGLGKQQGLFFPLDLPEFELTEID
                     HLLEQDFVTRSSRILSAFIGEEVPETALKKRVQAAFEFPAPVAKVTDDVSCLELFHGP
                     TLAFKDFGGRFMAQMLAEVAGEQPVTILTATSGDTGAAVAHAFYGLKNVRVVILYPQG
                     KISPLQEKLFCTLGGNIHTVAIDGDFDACQALVKQAFDDQELKDALHLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVISVPSGNFGDLTAGLLAKSLGLPVKRFIAATNAN
                     DTVPRFLTSGQWQPHATVATLSNAMDVSQPNNWPRVEELFRRKVWQLKELGHAAVSDE
                     TTKDTMRELAELGYISEPHAAIAYRALRDQLQEGEFGLFLGTAHPAKFKESVEAILGQ
                     ELPLPKALALRAELPLLSHTLPASFGELRKFLMGLPA"
     terminator      5658..5681
BASE COUNT     1190 a   1833 c   1953 g   1214 t
ORIGIN
        1 gtcgacagcg aggcgcatct gcagccggcg gcgcgctggg tggcacatgg ggccggcgag
       61 atcctcgacg gcgtgcaaac gttcgccacg ctggaacagg cgctggcgga tgtggatttt
      121 accgtcgcca ccaccgcccg cagtcgcgcg cgttttcact actactgcac gccgcctgag
      181 ctgctggaac agctgagtga gcgtaagcag tgggtcggcc aggccgcgct ggtattcggc
      241 cgcgaggatt ccggcctgac caacgaagag ctggcgctgg cggatttgct gaccggcgtg
      301 ccgatgcagg cggattatcc gtcgctcaat ctgggccagg cggtgatggt gtactgctac
      361 cagttggcga gcctgatggg cgtcaacgcc gcgccgcagg aggctgcggc gcctgaaggg
      421 caactcaggg cgttgcgcca ccgcgccgat gctttgttgg atgccctgga ggtcggcgat
      481 gaccaaaaat tgcgcgactg gctgcaccag cgcctcggtg cgttgccgca gcgagatacg
      541 gcgatgctgc atacgctgct gcacgacatc gaaaaaaaac tcgccaagtg atagcggctc
      601 cagcaggcgg ttactcagtg tgatccacta ttaaaccggc tttaggttgt ttttttgttc
      661 tgtcattttg cttttcacct gttgcatcgc gcattaccgc cgctgaacgg aaaataagca
      721 aaaataagaa atcgtttgac ttgggatagc gattgcttta accaatagag gggacagagt
      781 atttcatgag acagacagac aacatgcgca atatcagcct gactaccaca attattacca
      841 ccaccgatac cacaggtaac ggggcgggct gacgcgcaca ggaaacacag aaaaaagccc
      901 gcacctaatc agtgcgggct tttttttcgg cttaaattcg gagagatcac aaatgcgagt
      961 gctgaaattt ggcggaacct cggtagcgaa tgcggaacgt tttctgcgcg tcgccgacat
     1021 catggagagt aacgcgcgtc agggacaggt agccacggtc ttgtccgccc ccgcaaagat
     1081 caccaaccat ctggtggcga tgatcgacaa aacggtggcg ggccaggaca ttctgccgaa
     1141 tatgagcgac gccgagcgga tctttgccga cctgctgagc ggactggcgc aggcgctgcc
     1201 gggctttgaa tacgatcgtt tgaaaggcgt ggtcgatcag gaattcgcgc agctcaaaca
     1261 ggtgctgcat ggcgtctcgc tgctggggca gtgcccggac agcgtgaacg cggcgatcat
     1321 ctgccgtggc gaaaagctct ccatcgccat catggaaggg gtgttccgcg ccaagggtta
     1381 tccggtcacg gtgatcaacc cggtggagaa actgctggcg cagggccact acctggagtc
     1441 caccgtggac atcgccgagt ctacgctgcg catcgccgcc gcggcgatcc cggccgatca
     1501 catcgtgctg atggccggtt tcaccgccgg taatgataag ggcgagctgg tggtgctggg
     1561 ccgcaacggc tccgactatt ccgccgcggt gctggccgcc tgcctgcgcg ccgactgttg
     1621 cgagatctgg accgacgtcg acggcgttta tacctgcgat ccgcgcaccg tgccggacgc
     1681 caggttactg aaatcgatgt cgtaccagga agcgatggag ctttcctatt tcggcgccaa
     1741 agtgttacac ccccgcacca tcactccgat tgcccagttt caaatccctt gcctgattaa
     1801 aaacacctcc aacccgcagg cccccggcac gctgatcggc aaagacagca ccgatgccga
     1861 tatgccggtg aagggcatca ccaacctgaa caacatggcg atgatcaacg tctccggccc
     1921 gggcatgaaa ggcatggtcg gcatggccgc acgggtcttc gccgtgatgt cgcgcgccgg
     1981 catttcggtg gtgctgatca cccaatcctc ttccgaatac agcatcagct tctgcgtgcc
     2041 gcagggtgaa ctgcagcgcg cccgtcgcgc gctggaagag gagttctatc tggagctgaa
     2101 agacggcgtg ctggatccgc tggacgtgat ggaacgcctg gcgatcatct cggtggttgg
     2161 cgacggcatg cgcaccctgc gcggcatttc cgcacgcttc ttctccgcgc tggcgcgcgc
     2221 caacatcaat atcgttgcca tcgcccaagg gtcttccgaa cgttccattt cggtggtggt
     2281 cagcaatgat tccgccacca ccggcgtgcg cgtcagccac cagatgctgt tcaacaccga
     2341 tcaggtgatc gaagtgttcg tcatcggcgt cggcggcgtc ggcggggcgc tgatcgagca
     2401 gatctatcgc cagcagccgt ggctgaagca aaaacatatc gatctgcggg tgtgcggcat
     2461 cgccaactcg cgcgtgatgc tgaccaatgt gcacggcatc gcgctggaca gctggcgcga
     2521 cgcgctggcc ggcgcgcagg agccgttcaa tctcggccgt ctgatccggc tggtgaagga
     2581 atatcacctg ctgaacccgg tgatcgtcga ctgtacctcc agtcaggcgg tggccgatca
     2641 gtacgtggac ttcctggcgg acggcttcca tgtggtgacg ccgaacaaaa aggccaacac
     2701 ctcgtcgatg aactattatc agcaactgcg cgcggccgcc gccggttcgc atcgcaagtt
     2761 cctgtacgac accaacgtcg gtgccggtct gccggtgatt gagaacctgc aaaacctgct
     2821 gaacgccggt gatgaactgg tacgtttctc cggtatcctg tccggctcgc tgtcctttat
     2881 cttcggcaag ctggacgaag ggctgtcgct gtcggcggcg accctgcagg cccgggccaa
     2941 cggctacacc gagccggatc cgcgcgacga tctgtccggg atggatgtgg cgcgtaagct
     3001 gctgatcctg gcgcgcgagg ccggttacaa actggaactg agcgatatcg aggtcgagcc
     3061 ggtgctgccg ccgtccttcg acgcgtcggg cgacgtggac accttcctgg cgcggttgcc
     3121 ggagctggat aaagagttcg cgcgcaacgt ggctaacgcc gccgagcaag gcaaggtgct
     3181 gcgctatgtc ggcctgatcg acgaagggcg ctgcaaggtg cgcattgagg cggtggacgg
     3241 caacgatccg ttgtataaag tgaagaacgg cgagaacgcg ctggccttct acagccgcta
     3301 ctatcagccg ttgccgctgg tgctgcgcgg ctacggcgcc ggtaacgatg tgactgcagc
     3361 gggcgtattc gccgatctgc tgcgcacact gtcatggaag ttgggagttt aatatggtta
     3421 aggtgtatgc accggcctcg atcggcaacg tcagcgtcgg tttcgacgtg ctgggcgcgg
     3481 cggtttcgcc gatcgacggc acgctgctgg gcgactgtgt cagcgtcgaa gccgccgaga
     3541 cgttcagttt gcagaatgcc gggcgcttcg tcagcaagct gccggccgag ccgaaagaga
     3601 acatcgttta tcaatgctgg gagcgcttct gccaggagat cggccgcgaa gtgccggtgg
     3661 cgatgcgttt ggaaaagaac atgccgatcg gatcggggct ggggtccagc gcctgttcgg
     3721 tggtcgccgg gctgatggcg atgaacgaat tctgcgatcg tccgttggat aagacgacgc
     3781 tgctcggcct gatgggcgag ctggaagggc gcatttccgg cagcgtgcat tacgacaacg
     3841 tggcgccttg ctatctgggc ggcctgcagc tgatgctgga agaagaaggc attatcagcc
     3901 aggaggtgcc gtgctttgac gattggctgt gggttatggc ctatccgggc atcaaagtgt
     3961 ccaccgccga agcgcgcgcc attttacccg cgcaataccg tcgtcaggat tgcatcagcc
     4021 atggccgcta tctggccggc tttattcacg cctgccatac ccggcagccg cagctggccg
     4081 ccaaattgat gcaggacgtg atcgccgaac cgtaccgcac ccggctgttg ccgggcttcg
     4141 ccgaagcgcg caaggcggcg caggagatcg gcgcgttggc ctgcggcatc tccggctctg
     4201 gcccgacgct gttcgccgtc tgcaatgacg gcgccaccgc gcaacgcatg gccgcctggc
     4261 tgcaacaaca ctatctgcaa aacgacgaag gttttgttca tatttgccgt ctggataccg
     4321 caggcgctcg actactggga taacgcatga aactgtacaa ccttaaggat cacaacgagc
     4381 aggtcagctt cgcgcaggcg atcaaacaag gcctgggcaa gcaacagggg ctgttcttcc
     4441 cgctggatct gccggagttc gaactgaccg aaatcgacca cctgctggag caggattttg
     4501 tcacccgcag cagccgcatt ttgtcggcct ttatcggcga agaggtgccg gaaacggcgc
     4561 tgaagaaacg cgtgcaggcc gcctttgagt tcccggcgcc ggtggcgaaa gtgaccgatg
     4621 acgtctcctg cctggagctg ttccacggcc cgacgctggc gttcaaagac ttcggcggcc
     4681 gctttatggc gcagatgctg gcggaagtgg ccggcgagca gccggtgacc attctgaccg
     4741 ccacctccgg cgacaccggc gccgccgtgg cgcacgcctt ctacggcctg aaaaacgtgc
     4801 gggtggtgat cctgtatccg cagggcaaaa tcagcccgct gcaggaaaag ctgttctgca
     4861 ccctgggcgg caacattcac accgtggcga tcgacggtga cttcgatgct tgtcaggcgc
     4921 tggtgaagca ggcgttcgac gatcaggaat tgaaagacgc gctgcacctg aactcggcca
     4981 actcgattaa catcagccgt ttgctggcgc agatttgcta ctacttcgaa gcggtggcgc
     5041 agctgccgca ggaagcgcgc aatcagctgg tgatttcggt gccgagcggc aacttcggcg
     5101 atctgaccgc cgggctgctg gccaaatcgc tgggcctgcc ggtgaagcgc ttcatcgccg
     5161 ccaccaacgc caacgacacc gtgccgcgct tcctgaccag cggccagtgg cagccgcacg
     5221 ctacggtcgc gacgctgtcc aacgccatgg acgtcagcca gccgaacaac tggccgcgcg
     5281 tggaagagct gttccgtcgc aaggtctggc agttgaagga gctgggtcac gcggcggtca
     5341 gcgacgagac caccaaagac acgatgcgtg agctggccga gctgggctat atctccgaac
     5401 cgcatgcggc catcgcctac cgcgcgctgc gtgatcagtt gcaggaaggg gagttcggcc
     5461 tgttcctcgg caccgcgcat ccggccaagt ttaaagagag tgtggaagcg atactcggcc
     5521 aggagttgcc gttgccgaaa gcgctggcgc tgcgggctga gctgccgctg ctgtcgcaca
     5581 cgctgccggc cagcttcggc gagctgcgca agttcctgat ggggctgcct gcctgaagtt
     5641 cggcaggcta aaatgcgaaa agccgtcttg cgacggcttt tttttatgcc tgctccgggc
     5701 gtttgaacac cagttcattg ccttgcgagg cggcttcgtc gaaggcgtag ccttccaggt
     5761 tgaagtccgc cagctgctcg ctgcgcgtca ggcggttttt gatgataaag cggctcatca
     5821 acccgcgcgc tttcttggcg tagaagctga tgaccttgaa cttgccgttc ttctcgtcga
     5881 ggaacaccgg tttgatcagc gagccgtgca gcttggccgg cttcaccgct ttgaaatact
     5941 cgtctgacgc caggttgacc accacgtcgt cgccctgttg ctccagcgct tcgttgagct
     6001 ttttggtgat ctgatcgccc cagaagctgt acagatcttt gcccttcggg ttttccagct
     6061 tgatgcccat ctccagccgg tacggcatca tcagatcgag cgggcgcagt acgccataca
     6121 ggccggacag catgcgcaga tgctgctggg cgaagtcgaa atcggcttcg ttgaagtcct
     6181 gcgcctgcag
//