From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Plasmid (from S. flexneri) virH gene, complete cds.
Date: 9 Jan 93 05:47:49 GMT
Distribution: bionet
Checksum: 24144 6

LOCUS       SHFVIRH      2952 bp ds-DNA             BCT       09-JAN-1993
DEFINITION  Plasmid (from S. flexneri) virH gene, complete cds.
ACCESSION   D10999
KEYWORDS    virulence region-4.
SOURCE      Plasmid (from Shigella flexneri) DNA.
  ORGANISM  Plasmid
            Prokaryotae; Bacteria.
REFERENCE   1  (bases 1 to 2952)
  AUTHORS   Sasakawa,C.
  TITLE     A virulence locus, virH, on the large plasmid of Shigella flexneri
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
COMMENT     Submitted (27-Apr-1992) to DDBJ by: Chihiro Sasakawa Institute of
            Medical Science University of Tokyo 4-6-1 Shirokanedai, Minato-ku
            Tokyo 108 Japan Phone:  03-3443-8111x252 Fax:    03-3443-3893.
FEATURES             Location/Qualifiers
     CDS             287..2347
                     /function="excretion of IpaB, IpaC and IpaD"
                     /gene="virH"
                     /note="gtg start codon"
                     /product="virulence protein"
                     /codon_start=1
                     /translation="MIQSFLKQVSTKPELIILVLMVMIIAMLIIPLPTYLVDFLIGLN
                     IVLAILVFMGSFYIERILSFSTFPSVLLITTLFRLALSISTSRLILVDADAGKIITTF
                     GQFVIGDSLAVGFVIFSIVTVVQFIVITKGSERVAEVAARFSLDGMPGKQMSIDADLK
                     AGIIDAAGAKERRSILERESQLYGSFDGAMKFIKGDAIAGIIIIFVNLIGGISVGMSQ
                     HGMSLSGALSTYTILTIGDGLVSQIPALLISISAGFIVTRVNGDSDNMGRNIMSQIFG
                     NPFVLIVTSALALAIGMLPGFPFFVFFLIAVTLTALFYYKKVVEKEKSLSESDSSGYT
                     GTFDIDNSHDSSLAMIENLDAISSETVPLILLFAENKINANDMEGLIERIRSQFFIDY
                     GVRLPTILYRTSNELKVDDIVLLINEVRADSFNIYFDKVCITDENGDIDALGIPVVST
                     SYNERVISWVDVSYTENLTNIDAKIKSAQDEFYHQLSQALLNNINEIFGIQETKNMLD
                     QFENRYPDLLKEVFRHVTIQRISEVLQRLLGENISVRNLKLIMESLALWAPREKDVIT
                     LVEHVRASLSRYICSKIAVSGEIKVVMLSGYIEDAIRKGIRQTSGGSFLNMDIEVSDE
                     VMETLAHALRELRNAKKNFVLLVSVDIRRFVKRLIDNRFKSILVISYAEIDEAYTINV
                     LKTI"
BASE COUNT      908 a    401 c    603 g   1040 t
ORIGIN      
        1 aagcttgcta aaagatggca gtttaagtgc agagcagcta ttgctaactt tattatatat
       61 ttttcaatat ccaagtgaaa gtgagcaaat tcttacttct gttatagaag tatcacgagc
      121 cagtcatgag gattctgtag tgtatcaaac atatctatct tctgttaatg aaagtcctca
      181 tgatatattt aaaagtgaaa gtgaaagaga aattgcgatc aatattctac gagagcttgt
      241 cacaagtgca tacaagaaag agctttctag ataacaggag ataaaagtga tccagtcttt
      301 tcttaagcaa gtaagtacta agcctgaatt aattatatta gtgcttatgg ttatgatcat
      361 tgctatgttg atcataccat tacctacata ccttgttgat tttttaatag ggcttaatat
      421 tgtacttgct attctcgttt ttatgggatc attttatatt gaaagaatat tgagtttttc
      481 tacattccct tcggttttgt tgataacgac attatttcgt ctcgctttat caataagtac
      541 tagtcgacta attttagttg atgcggatgc gggtaaaatt attactacat ttggccagtt
      601 tgtcattggt gatagtctgg ctgtaggttt tgttattttt tcaatagtaa ctgttgtgca
      661 gttcattgtt ataacaaaag gatctgaaag ggttgcggag gtagctgctc gcttttctct
      721 tgacggtatg ccggggaaac aaatgagcat tgatgcggat ttaaaggctg gaattattga
      781 tgccgcagga gctaaagaaa gacgcagtat tctggaacgt gagagtcaat tgtatggttc
      841 atttgatgga gcaatgaagt tcattaaagg tgatgcaatt gctggcatca ttattatttt
      901 tgtcaattta ataggaggta tttctgttgg tatgagtcaa catggaatgt ccctatctgg
      961 tgcattatct acttatacca tactaactat tggtgacgga ttggtctctc agattcctgc
     1021 tctgttaatt tctataagtg caggatttat cgttacaaga gttaatggtg atagcgataa
     1081 tatgggacgt aacataatgt cccagatatt tgggaatcct tttgttctca ttgttacatc
     1141 agctcttgcc ttggcgatag ggatgttgcc aggttttccg ttttttgttt ttttcctgat
     1201 agcagttact ttgacggctt tattctatta taaaaaggtc gtagaaaaag aaaaaagtct
     1261 gtctgagtct gattctagtg gctatactgg tacatttgat attgataata gccatgactc
     1321 atctttggca atgatagaaa atctggatgc tattagttca gaaaccgttc ctttaatttt
     1381 attatttgcc gaaaataaga taaatgcaaa tgatatggaa ggtcttattg aaaggataag
     1441 aagtcagttc tttattgatt atggtgtcag gcttccaact attttatata gaacaagcaa
     1501 tgagcttaag gtcgatgata ttgttttgtt aataaatgag gtgcgtgccg atagttttaa
     1561 tatatacttt gacaaagtgt gtattacaga tgaaaatgga gatatagatg ctctaggtat
     1621 tcctgttgtt tcaacttcat ataatgaacg tgttatttct tgggtagacg tttcatatac
     1681 agaaaatcta actaatattg atgctaaaat taaaagtgct caagatgaat tttatcacca
     1741 gttgtcacaa gctttattaa acaacataaa tgagattttt ggtatacaag aaacaaaaaa
     1801 tatgttagat cagtttgaaa atcggtatcc tgatctatta aaggaagtct tccgacatgt
     1861 gactatacag agaatttctg aggtattaca aagattgctt ggagaaaata tttctgttcg
     1921 caatttaaaa cttattatgg agtctttggc gctttgggct cccagagaaa aagatgtcat
     1981 aacattagtt gaacatgtcc gtgcatcact ttctaggtat atttgtagta aaatagctgt
     2041 ttctggtgag attaaagttg tgatgctttc cggatatatt gaggatgcaa taagaaaagg
     2101 gataaggcaa acctctggtg gctctttctt gaatatggat atagaggttt cggatgaggt
     2161 aatggaaact ttagcacatg ctttgagaga attgagaaat gcaaaaaaaa atttcgttct
     2221 tttggtatca gtagatatac gtaggtttgt taaaagactt atagataaca gatttaagag
     2281 tatactcgtt atatcgtatg ctgagattga tgaagcatat accattaatg tattaaagac
     2341 tatttagtga ggtttaaata tgagtaacat taatttagtt caattagtta gagatagtct
     2401 tttcacgatt ggttgtcctc catcaattat tacagattta gacagccatt cagcgattac
     2461 catttctttg gatagtatgc ctgctattaa tatagctctt gtcaatgagc aggttatgtt
     2521 atgggctaat tttgatgcac caagtgatgt aaaacttcag tcatctgctt ataacatatt
     2581 aaatttaatg ttaatgaact ttagttatag cattaatgag ctggtagagc ttcatcgttc
     2641 tgatgagtac ttacagttaa gagttgttat taaagatgac tacgttcacg atgggattgt
     2701 ttttgcagag attctgcatg agttttatca gaggatggaa atcttaaatg gggtcttata
     2761 atcaatgagc tatacaaaat tgctcactca attatctttt cctaatagaa tctcggggcc
     2821 aatcttggaa acaagtctta gcgatgtttc gattggtgag atttgtaaca ttcaggctgg
     2881 aattgaaagt aatgaaattg ttgcaagagc tcaggttgta ggatttcatg atgaaaaaac
     2941 aatattaagc tt
//