From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Tomato ribulose 1,5-bisphosphate carboxylase/oxygenase small
Date: 3 Jan 93 22:22:35 GMT
Distribution: bionet
Checksum: 51031 9

LOCUS       TOMRBCO      4124 bp ds-DNA             PLN       23-DEC-1992
DEFINITION  Tomato ribulose 1,5-bisphosphate carboxylase/oxygenase small
            subunit gene, complete cds.
ACCESSION   D11112
KEYWORDS    rbcS multigene family;
            ribulose 1,5-bisphosphate carboxylase/oxygenase small subunit.
SOURCE      Lycopersicon esculentum (cultivar VFNT1221, cherry line) DNA.
  ORGANISM  Lycopersicon esculentum
            Eukaryota; Plantae; Embryobionta; Magnoliophyta; Magnoliopsida;
            Asteridae; Solanales; Solanaceae.
REFERENCE   1  (sites)
  AUTHORS   Sugita,M.
  TITLE     Genomic organization, sequence analysis and expression of all five
            genes encoding the small subunit of ribulose-1,5-bisphosphate
            carboxylase/oxygenase from tomato
  JOURNAL   Mol. Gen. Genet. 209, 247-256 (1987)
  STANDARD  full automatic
REFERENCE   2  (bases 1 to 4124)
  AUTHORS   Sugita,M. and Hidaka,T.
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
COMMENT     Submitted (13-May-1992) to DDBJ by: Mamoru Sugiura Center for Gene
            Research Nagoya University Chikusa-ku, Nagoya 464-01 Japan Phone:
            052-781-5111 x3453 Fax:    052-782-3849

               [1]  sites.
FEATURES             Location/Qualifiers
     misc_feature    2498..2734
                     /note="AT-rich region (84% AT)"
     misc_feature    2815..2838
                     /note="AT-rich motif"
     misc_feature    3112..3132
                     /note="AT-rich motif"
     misc_feature    3136..3161
                     /note="AT-rich motif"
     misc_signal     3193..3198
                     /note="I-Box"
     misc_signal     3201..3211
                     /note="Box II light regulatory sequence"
     CAAT_signal     3248..3257
     misc_signal     3272..3279
                     /note="light regulatory sequence"
     misc_feature    3298..3306
                     /note="LRE-motif"
     TATA_signal     3310..3317
     misc_feature    3343
                     /evidence="EXPERIMENTAL"
                     /note="transcriptional start site"
     exon            3351..3527
                     /number=1
                     /gene="Rbcs-3B"
     intron          3528..3619
                     /number=1
     exon            3620..3754
                     /number=2
                     /gene="Rbcs-3B"
     intron          3755..3836
                     /number=2
     exon            3837..4067
                     /gene="Rbcs-3B"
     CDS             join(3351..3527,3620..3754,3837..4067)
                     /EC_number="4.1.1.39"
                     /gene="Rbcs-3B"
                     /product="ribulose 1,5-bisphosphate carboxylase/oxygenase
                     small subunit"
                     /codon_start=1
                     /translation="MASSIVSSAAVATRGNGAQASMVAPFTGLKSTASFPVSRKQNLD
                     ITSIASNGGRVSCMQVWPPINMKKYETLSYLPDLSDEQLLSEIEYLLKNGWVPCLEFE
                     TEHGFVYRENHKSPGYYDGRYWTMWKLPMFGCTDATQVLAEVQEAKKAYPQAWVRIIG
                     FDNVRQVQCISFIAYKPEGY"
BASE COUNT     1466 a    699 c    722 g   1237 t
ORIGIN      Chromosome 2.
        1 aagcttcttt ttccgcttgt caacctacat ttacaattca aactatgcac tgaaaagtac
       61 tagtaatgca taatagatgc tattaagttt gattgcaaaa aagacgtaca atcatcaaat
      121 aaacatgcct aacaataatg acaatatttt caacttccaa acttatgata agaagataaa
      181 tcataaccat tatgaactgc aaattactat cattcaaaca aatccatgat attgtagcgc
      241 aaagaagaca cacaacatgt caaattgtta gctttctact ttttcctgat tgattaatat
      301 ggccatccat cgatctttta taagggacac aaacttatta gtgtttcctg ttgtgcattt
      361 tatccaacac aaagaagttc ggctaattgt aatgttcctg caaaatcagc cacactttat
      421 tcatttgagt cccactggac aaaagtctgt ctgtattaca gattttaagt atgtattata
      481 aaagtcaacc aatcagcctt taaacttgaa ccctacttca gatcaagcaa tcacccaacc
      541 agtttcaata acaatcttat ctaagaattc agttccaaga acaaacttat ctgaagaata
      601 aatgtaaaaa tactcgatgc taaaagaaac tgaagttaac gtcatcctgt atgtggtaat
      661 atatcgtata gatactgttc taaaaaaacc tgttatttga tgggtgttag ttaatagaaa
      721 aaaattcgac taaccagaat atcaaggagc atttgaattg ccgcgttgct tttcaatttc
      781 ttgcttttgt ttctcagcaa gtttatccaa agcagcttcc agagcatccc gacctccaat
      841 aagcaacctg gtgatgacct ctggatctct ctcaaattgt gcctcctcga actcttttcg
      901 gacattttct ctaagaacat cccgccaggg aatgccttga gagttggccc acatgaagaa
      961 gcgggttgcg cggatgacat ctcggtagag acttagagcc tcgcgtcgac tgctagtgag
     1021 tcgttgtcta tttaggagct cgttctcgtc atcatcaagg ttcttctcct ttttgaccac
     1081 atgtctatcc aatagttcct ccatagtgtc tggaccatgg tgcaggaggc aatagtgatg
     1141 caagagccat cttgacttga aactatgatc caaagcagtt gaaagatttc ggaactttcg
     1201 aatattagca ttcatctgaa acctcgactt gactggtaca gaaagagaaa caagacttta
     1261 gagaaatcgt acttcatcat atacttcaca cgagaaacgc atgtagatca acatgaagtg
     1321 aaaaatggtc caagttaaaa ataaacttgt taagaaggtc agtaacatcc aaacagaaag
     1381 tcttgctttt cttaagaatg ctatcaaaca caatagccag agaacagaag tggtgcgcat
     1441 cttctggtat gagagatact acaacagcaa caacaacata caacataccc gagaaatctc
     1501 acaaagtggg ggtatgccag atactacatg attggaatat attccagctg attcaatact
     1561 ttctacagca atgcacgaca ggaataaaga tgaacaaaat caaaaaaaaa aaaagaactt
     1621 ctctttttcc atttgggcgc gtaatgaaag agctccatgt ggaagaatgg gagaacccac
     1681 atgcttattc cattcagttt aatcagaatt caagcataat caatttggaa aaagcataac
     1741 caaaacagta tagaacagag aaaatagata aattagaaga cagcaacact ataaaaagaa
     1801 caatttactc ttcaccggaa cttctcctaa caaaatgtaa tcaacaggta ctggcaacat
     1861 aacacaaaaa aaatttgact acaacataca caattgagtt cacacaaaaa aaaaattaaa
     1921 atctgaaagt agccctaatt acagaaaaaa gattgaagat gtaacctgtt gtaggtatct
     1981 gcaaattagt gagtggaaaa gagagaatgc acaacaatac tttgggcaat tagcgttgta
     2041 ggaacggaag aagaattgga aaatagtgtt tggcaattgc gggtcgaaaa atgggttaaa
     2101 atggcaattg cgggtagaga agatgggcca taaatggtta caaaatagat atgggctcaa
     2161 catattttct gggcagccaa ttttaaaggc attttccttt gaggaaataa tttctttgga
     2221 cttcagaata tgagttgaaa gtaataattc taataatgaa attaaacaag gatgattaaa
     2281 tggcaacaaa atggagtaat atggataatc aacgcaacta tatagagaaa aaataatagc
     2341 gctaccatat acgaaaaata gtaaaaaatt ataataatga ttcagaataa attattaata
     2401 actaaaaagc gtaaagaaat aaattagaga ataagtgata caaaattgga tgttaatgga
     2461 tacttcttat aattgcttaa aaggaataca agatgggaaa taatgtgtta ttattattga
     2521 tgtataaaga atttgtacaa tttttgtatc aataaagttc caaaaataat ctttaaaaaa
     2581 taaaagtacc cttttatgaa ctttttatca aataaatgaa atccaatatt agcaaaacat
     2641 tgatattatt actaaatatt tgttaaatta aaaaatatgt cattttattt tttaacagat
     2701 attttttaaa gtaaatgtta taaattacga aaaagggatt aatgagtatc aaaacagcct
     2761 aaatgggagg agacaataac agaaatttgc tgtagtaagg tggcttaagt catcatttaa
     2821 tttgatatta taaaaattct aattagttta tagtctttct tttcctcttt tgtttgtctt
     2881 gtatgctaaa aaaggtatat tatatctata aattatgtag cataatgacc acatctggca
     2941 tcatctttac acaattcacc taaatatctc aagcgaagtt ttgccaaaac tgaagaaaag
     3001 atttgaacaa cctatcaagt aacaaaaatc ccaaacaata tagtcatcta tattaaatct
     3061 tttcaattga agaaattgtc aaagacacat acctctatga gttttttcat caattttttt
     3121 ttctttttta aactgtattt ttaaaaaaat attgaataaa acatgtccta ttcattagtt
     3181 tgggaacttt aagataagga gtgtgtaatt tcagaggcta ttaattttga aatgtcaaga
     3241 gccacataat ccaatggtta tggttgctct tagatgaggt tattgcttta ggtgaaacct
     3301 tatcatcatt atatatacaa ggggatacta gagaccaatt attgtcaaca atggcttcct
     3361 ctatagtttc ttcagctgct gttgccaccc gcggcaatgg tgcacaagct agcatggttg
     3421 cacccttcac tggactcaag tccactgctt ctttccctgt ttcaaggaag caaaaccttg
     3481 acattacctc cattgctagc aacggtggaa gagtcagttg catgcaggtt tgtgtgtgta
     3541 tatatatata tgtacaacaa aattcattga ctataatgct atactcgatt agctaattta
     3601 actatttata attgtatagg tgtggccacc aattaacatg aagaagtacg agactctgtc
     3661 gtaccttcct gatttgtccg acgagcaatt gctcagcgaa attgagtacc tattgaaaaa
     3721 tggatgggtt ccttgcttgg aattcgagac tgaggttaac ctctgttttt aaaatttata
     3781 tattagctag tgtgttgata tgttgtgtta atagtgttgt gggatatcat gtgcagcacg
     3841 gatttgtgta ccgtgagaac cataagtcac caggatacta cgatggcaga tactggacca
     3901 tgtggaagtt gcccatgttt gggtgcactg atgcaaccca ggtcttggct gaggtgcagg
     3961 aggcaaagaa ggcttaccct caggcatggg tccgtatcat cggattcgac aatgttcgtc
     4021 aagtgcagtg catcagtttc atcgcttaca agcccgaagg atactaaaat gccaattttt
     4081 aattatgtaa tgtataactg accctatgta tttaggggaa gctt
//