From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Bacillus subtilis gene for neutral protease(monomer), complete cds.
Date: 21 Jul 92 10:03:35 GMT
Distribution: bionet
Checksum: 37176     6

LOCUS       BACNP        2046 bp ds-DNA             BCT       21-JUL-1992
DEFINITION  Bacillus subtilis gene for neutral protease(monomer), complete cds.
ACCESSION   D10773 D01147
KEYWORDS    neutral protease.
SOURCE      Bacillus subtilis (strain K02, sub_species amylosacchariticus)
            (library: pUC18) DNA, clones pSNE3 and pSNB3.
  ORGANISM  Bacillus subtilis
            Prokaryotae; Firmicutes; Endospore-forming rods and cocci;
            Bacillaceae.
REFERENCE   1  (sites)
  AUTHORS   Tsuru,D., Yamamoto,T. and Fukumoto,J.
  TITLE     Studies on Bactgerial Protease: Part XIII. purification,
            crystallization and some enzymatic properties of neutral protease
            of Bacillus subtilis var. amylosacchariticus
  JOURNAL   Agric. Biol. Chem. 30, 651-658 (1966)
  STANDARD  full staff_review
REFERENCE   2  (bases 1 to 2046)
  AUTHORS   Yoshimoto,T., Oyama,H., Takeshita,T., Higashi,H., Xu,S. and
            Tsuru,D.
  TITLE     Nucleotide sequence of the neutral protease gene from Bacillus
            subtillis var, amylosacchariticus
  JOURNAL   J. Ferment. Bioeng. 70, 370-375 (1991)
  STANDARD  full staff_review
COMMENT     These data kindly submitted in computer readable form by:
            Tadashi Yoshimoto
            School of Pharmaceutical Science
            Nagasaki University
            1-14 Bunkyo-machi
            Nagasaki 852
            Japan
            Phone:  0958-47-1111 x2551
            Fax:    0958-44-6774
            [1]  sites.
FEATURES             Location/Qualifiers
     -35_signal      210..215
     -10_signal      233..238
     RBS             267..274
     CDS             282..1847
                     /note="The possible initiation codon is GTG."
                     /product="prepro neutral protease"
                     /codon_start=1
                     /translation="MGLGKKLSVAVAASFMSLSISLPGVQAAEGHQLKENQTNFLSKN
                     AIAQSELSAPNDKAVKQFLKKNSNIFKGDPSKRLKLVESTTDALGYKHFRYAPVVNGV
                     PIKDSQVIVHVDKSDNVYAVNGELHNQSAAKTDNSQKVSSEKALALAFKAIGKSPDAV
                     SNGAAKNSNKAELKAIETKDGSYRLAYDVTIRYVEPEPANWEVLVDAETGSILKQQNK
                     VEHAAATGSGTTLKGATVPLNISYEGGKYVLRDLSKPTGTQIITYDLQNRQSRLPGTL
                     VSSTTKTFTSSSQRAAVDAHYNLGKVYDYFYSNFKRNSYDNKGSKIVSSVHYGTQYNN
                     AAWTGDQMIYGDGDGSFFSPLSGSLDVTAHEMTHGVTQETANLIYENQPGALNESFSD
                     VFGYFNDTEDWDIGEDITVSQPALRSLSNPTKYNQPDNYANYRNLPNTDEGDYGGVHT
                     NSGIPNKAAYNTITKLGVSKSQQIYYRALTTYLTPSSTFKDAKAALIQSARDLYGSTD
                     AAKVEAAWNAVGL"
     mat_peptide     945..1844
                     /EC_number="3.4.24.4"
                     /product="neutral protease"
                     /codon_start=1
     repeat_region   1851..1927
                     /rpt_type=inverted
                     /rpt_unit=1851..1865
                     /rpt_unit=1870..1877
                     /rpt_unit=1878..1896
                     /rpt_unit=1904..1921
                     /rpt_unit=1922..1927
                     /note="it seems to construct a stem-loop structure."
BASE COUNT      660 a    465 c    398 g    523 t
ORIGIN
        1 ggggatcctc tagagtccct cattcggtta gacagcggac ttttcaaaaa gtttcaagat
       61 gaaacaaaaa tatctcatct tccccttgat atgtaaaaaa cataactctt gagtgaacca
      121 tcacatgaca cttgactcat cttgatatta ttcaacaaaa acaaacacag gacaatacta
      181 tcaattttgt ctagttatgt tagtttttgt tgagtattcc agaatgctag tttaatataa
      241 caatataaag ttttcagtat tttcaaaaag ggggatttat tgtgggttta ggtaagaaat
      301 tgtctgttgc tgtcgctgct tcgtttatga gtttatcaat cagcctgcca ggtgttcagg
      361 ctgctgaagg tcatcagctt aaagagaatc aaacaaattt cctctccaaa aatgcgattg
      421 cgcaatcaga actctctgca ccaaatgaca aggctgtcaa gcagtttttg aaaaagaaca
      481 gcaacatttt taaaggtgac ccttccaaaa ggctgaagct tgttgaaagc acgactgatg
      541 cccttggata caagcacttt cgatatgcgc ctgtcgttaa cggagtgcca attaaagatt
      601 cgcaagtgat cgttcacgtc gataaatccg ataatgtcta tgcggtcaat ggtgaattac
      661 acaatcaatc tgctgcaaaa acagataaca gccaaaaagt ctcttctgaa aaagcgctgg
      721 cactcgcttt caaagctatc ggcaaatcac cagacgctgt ttctaacgga gcggccaaaa
      781 acagcaataa agccgaattg aaagcgatag aaacaaagga cggcagctat cgtcttgctt
      841 acgacgtgac gattcgctat gtcgagcctg aacctgcaaa ctgggaagtc ttagttgacg
      901 ctgaaacagg cagcatttta aaacagcaaa ataaagtaga acatgccgcc gccactggaa
      961 gcggaacaac tctaaagggc gcaactgttc ctttgaacat ctcttatgaa ggcggaaaat
     1021 atgttctaag agatctttca aaaccaacag gcacccaaat catcacatat gatttgcaaa
     1081 acagacaaag ccgccttccg ggcacgcttg tctcaagcac aacgaaaaca tttacatctt
     1141 catcacagcg ggcagccgtt gacgcacact ataacctcgg taaagtgtac gattattttt
     1201 attcaaactt taaacgaaac agctatgata acaaaggcag taaaatcgtt tcttccgttc
     1261 actacggcac tcaatacaat aacgctgcat ggacaggaga ccagatgatt tacggtgatg
     1321 gcgacggttc attcttctct ccgctttccg gctcattaga tgtgacagcg catgaaatga
     1381 cacatggcgt cacccaagaa acagccaact tgatttatga aaatcagcca ggtgcattaa
     1441 acgagtcttt ctctgacgta ttcgggtatt ttaacgatac agaagactgg gacatcggtg
     1501 aagacattac ggtcagccag cctgctcttc gcagcctgtc caaccctaca aaatacaacc
     1561 agcctgacaa ttacgccaat tacagaaacc ttccaaacac agatgaaggc gattatggcg
     1621 gtgtacacac aaacagcgga attccaaaca aagccgctta caacaccatc acaaaacttg
     1681 gtgtatctaa atcacagcaa atctattacc gtgcgttaac aacgtacctc acgccttctt
     1741 ccacgttcaa agatgccaag gcagctctca ttcagtctgc ccgtgacctc tacggctcaa
     1801 ctgatgccgc taaagttgaa gcagcctgga atgctgttgg attgtaatat tagaaaaagc
     1861 ctgagatttc tcaggctcag actgtagaca aactggaaga aatccagttt gcctacagtc
     1921 ttttttattt tacaatggaa gtactacata acttgaggtg attgacatgc tttcgaaaca
     1981 taatcccatt caacgagatc aattagaaat gattacgtta aatcaattgg taccggccag
     2041 ccactt
//