From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human mRNA for ubiquitin activating enzyme E1
Date: 4 Sep 92 10:04:28 GMT
Distribution: bionet
Checksum: 00298     8

LOCUS       HUMQAE1      3419 bp ss-RNA             PRI       04-SEP-1992
DEFINITION  Human mRNA for ubiquitin activating enzyme E1
ACCESSION   X56976
KEYWORDS    ubiquitin activating enzyme E1.
SOURCE      Homo sapiens RNA.
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 3419)
  AUTHORS   Kaneda,S.
  JOURNAL   Unpublished (1990)
  STANDARD  full automatic
REFERENCE   2  (sites)
  AUTHORS   Ayusawa,D., Kaneda,S., Itoh,Y., Sugasawa,K., Hanaoka,F., Koyama,H.,
            Yasuda,H., Matsuhashi,M. and Seno,T.
  TITLE     Mammalian ubiquitin-activating enzyme is a determinant of thermal
            tolerance and a regulator of macromolecular
  JOURNAL   Cell Struct. Funct. 17, 113-122 (1992)
  STANDARD  full staff_review
COMMENT     *source: cell_line=breast carcinoma ZR-75-1; *source: clone=
            pcHUBA-1; *source: chromosome=X Xp11.23;
            From EMBL    entry HSQAE1;  dated 10-AUG-1992.
FEATURES             Location/Qualifiers
     CDS             33..3209
                     /product="ubiquitin activating enzyme E1"
                     /evidence=EXPERIMENTAL
                     /codon_start=1
                     /translation="MSSSPLSKKRRVSGPDPKPGSNCSPAQSVLSEVPSVPTNGMAKN
                     GSEADIDEGLYSRQLYVLGHEAMKRLQTSSVLVSGLRGLGVEIAKNIILGGVKAVTLH
                     DQGTAQWADLSSQFYLREEDIGKNRAEVSQPRLAELNSYVPVTAYTGPLVEDFLSGFQ
                     VVVLTNTPLEDQLRVGEFCHNRGIKLVVAGTRGLFGQLFCDFGEEMILTDSNGEQPLS
                     AMVSMVTKDNPGVVTCLDEARHGFESGDFVSFSEVQGMVELNGNQPMEIKVLGPYTFS
                     ICDTSNFSDYIRGGIVSQVKVPKKISFKSLVASLAEPDFVVTDFAKFSRPAQLHIGFQ
                     ALHQFCAQHGRPPRPRNEEDAAELVALAQAVNARALPAVQQNNLDEDLIRKLAYVAAG
                     DLAPINAFIGGLAAQEVMKACSGKFMPIMQWLYFDALECLPQDKEVLTEDKCLQRQNR
                     YDGQVAVFGSDLQEKLGKQKYFLVGAGAIGCELLKNFAMIGLGCGEGGEIIVTDMDTI
                     EKSNLNRQFLFRPWDVTKLKSDTAAAAVRQMNPHIRVTSHQNRVGPDTERIYDDDFFQ
                     NLDGVANALDNVDARMYMDRRCVYYRKPLLESGTLGTKGNVQVVIPFLTESYSSSQDP
                     PEKSIPICTLKNFPNAIEHTLQWARDEFEGLFKQPAENVNQYLTDPKFVERTLRLAGT
                     QPLEVLEAVQRSLVLQRPQTWADCVTWACHHWHTQYSNNIRQLLHNFPPDQLTSSGAP
                     FWSGPKRCPHPLTFDVNNPLHLDYVMAAANLFAQTYGLTGSQDRAAVATFLQSVQVPE
                     FTPKSGVKIHVSDQELQSANASVDDSRLEELKATLPSPDKLPGFKMYPIDFEKDDDSN
                     FHMDFIVAASNLRAENYDIPSADRHKSKLIAGKIIPAIATTTAAVVGLVCLELYKVVQ
                     GHRQLDSYKNGFLNLALPFFGFSEPLAAPRHQYYNQEWTLWDRFEVQGLQPNGEEMTL
                     KQFLDYFKTEHKLEITMLSQGVSMLYSFFMPAAKLKERLDQPMTEIVSRVSKRKLGRH
                     VRALVLELCCNDESGEDVEVPYVRYTIR"
     polyA_signal    3387..3392
BASE COUNT      753 a    995 c    928 g    743 t
ORIGIN
        1 tggcggcggc gcgacccggg gaaccggcat tgatgtccag ctcgccgctg tccaagaaac
       61 gtcgcgtgtc cgggcctgat ccaaagccgg gttctaactg ctcccctgcc cagtccgtgt
      121 tgtccgaagt gccctcggtg ccaaccaacg gaatggccaa gaacggcagt gaagcagaca
      181 tagacgaggg cctttactcc cggcagctgt atgtgttggg ccatgaggca atgaagcggc
      241 tccagacatc cagtgtcctg gtatcaggcc tgcggggcct gggcgtggag atcgctaaga
      301 acatcatcct tggtggggtc aaggctgtta ccctacatga ccagggcact gcccagtggg
      361 ctgatctttc ctcccagttc tacctgcggg aggaggacat cggtaaaaac cgggccgagg
      421 tatcacagcc ccgcctcgct gagctcaaca gctatgtgcc tgtcactgcc tacactggac
      481 ccctcgttga ggacttcctt agtggtttcc aggtggtggt gctcaccaac acccccctgg
      541 aggaccagct gcgagtgggt gagttctgtc acaaccgtgg catcaagctg gtggtggcag
      601 gcacgcgggg cctgtttggg cagctcttct gtgactttgg agaggaaatg atcctcacag
      661 attccaatgg ggagcagcca ctcagtgcta tggtttctat ggttaccaag gacaaccccg
      721 gtgtggttac ctgcctggat gaggcccgac acgggtttga gagcggggac tttgtctcct
      781 tttcagaagt acagggcatg gttgaactca acggaaatca gcccatggag atcaaagtcc
      841 tgggtcctta tacctttagc atctgtgaca cctccaactt ctccgactac atccgtggag
      901 gcatcgtcag tcaggtcaaa gtacctaaga agattagctt taaatccttg gtggcctcac
      961 tggcagaacc tgactttgtg gtgacggact tcgccaagtt ttctcgccct gcccagctgc
     1021 acattggctt ccaggccctg caccagttct gtgctcagca tggccggcca cctcggcccc
     1081 gcaatgagga ggatgcagca gaactggtag ccttagcaca ggctgtgaat gctcgagccc
     1141 tgccagcagt gcagcaaaat aacctggacg aggacctcat ccggaagctg gcatatgtgg
     1201 ctgctgggga tctggcaccc ataaacgcct tcattggggg cctggctgcc caggaagtca
     1261 tgaaggcctg ctccgggaag ttcatgccca tcatgcagtg gctatacttt gatgcccttg
     1321 agtgtctccc tcaggacaaa gaggtcctca cagaggacaa gtgcctccag cgccagaacc
     1381 gttatgacgg gcaagtggct gtgtttggct cagacctgca agagaagctg ggcaagcaga
     1441 agtatttcct ggtgggtgcg ggggccattg gctgtgagct gctcaagaac tttgccatga
     1501 ttgggctggg ctgcggggag ggtggagaaa tcatcgttac agacatggac accattgaga
     1561 agtcaaatct gaatcgacag tttcttttcc ggccctggga tgtcacgaag ttaaagtctg
     1621 acacggctgc tgcagctgtg cgccaaatga atccacatat ccgggtgaca agccaccaga
     1681 accgtgtggg tcctgacacg gagcgcatct atgatgacga ttttttccaa aacctagatg
     1741 gcgtggccaa tgccctggac aacgtggatg cccgcatgta catggaccgc cgctgtgtct
     1801 actaccggaa gccactgctg gagtcaggca cactgggcac caaaggcaat gtgcaggtgg
     1861 tgatcccctt cctgacagag tcgtacagtt ccagccagga cccacctgag aagtccatcc
     1921 ccatctgtac cctgaagaac ttccctaatg ccatcgagca caccctgcag tgggctcggg
     1981 atgagtttga aggcctcttc aagcagccag cagaaaatgt caaccagtac ctcacagacc
     2041 ccaagtttgt ggagcgaaca ctgcggctgg caggcactca gcccttggag gtgctggagg
     2101 ctgtgcagcg cagcctggtg ctgcagcgac cacagacctg ggctgactgc gtgacctggg
     2161 cctgccacca ctggcacacc cagtactcga acaacatccg gcagctgctg cacaacttcc
     2221 ctcctgacca gctcacaagc tcaggagcgc cgttctggtc tgggcccaaa cgctgtccac
     2281 acccgctcac ctttgatgtc aacaatcccc tgcatctgga ctatgtgatg gctgctgcca
     2341 acctgtttgc ccagacctac gggctgacag gctctcagga ccgagctgct gtggccacat
     2401 tcctgcagtc tgtgcaggtc cccgaattca cccccaagtc tggcgtcaag atccatgttt
     2461 ctgaccagga gctgcagagc gccaatgcct ctgttgatga cagtcgtcta gaggagctca
     2521 aagccactct gcccagccca gacaagctcc ctggattcaa gatgtacccc attgactttg
     2581 agaaggatga tgacagcaac tttcatatgg atttcatcgt ggctgcatcc aacctccggg
     2641 cagaaaacta tgacattcct tctgcagacc ggcacaagag caagctgatt gcagggaaga
     2701 tcatcccagc cattgccacg accacagcag ccgtggttgg ccttgtgtgt ctggagctgt
     2761 acaaggttgt gcaggggcac cgacagcttg actcctacaa gaatggtttc ctcaacttgg
     2821 ccctgccttt ctttggtttc tctgaacccc ttgccgcacc acgtcaccag tactataacc
     2881 aagagtggac attgtgggat cgctttgagg tacaagggct gcagcctaat ggtgaggaga
     2941 tgaccctcaa acagttcctc gactatttta agacagagca caaattagag atcaccatgc
     3001 tgtcccaggg cgtgtccatg ctctattcct tcttcatgcc agctgccaag ctcaaggaac
     3061 ggttggatca gccgatgaca gagattgtga gccgtgtgtc gaagcgaaag ctgggccgcc
     3121 acgtgcgggc gctggtgctt gagctgtgct gtaacgacga gagcggcgag gatgtcgagg
     3181 ttccctatgt ccgatacacc atccgctgac cccgtctgct cctctaggct ggccccttgt
     3241 ccacccctct ccacacccct tccagcccag ggttcccatt tggcttctgg cagtggccca
     3301 actagccaag tctggtgttc cctcatcatc cccctacctg aacccctctt gccactgcct
     3361 tctaccttgt ttgaaacctg aatcctaata aagaattaat aactcccaaa aaaaaaaaa
//