From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Bovine nitric oxide synthase mRNA, complete cds.
Date: 26 Jan 93 05:46:30 GMT
Distribution: bionet
Checksum: 46947 8

LOCUS       BOVNIOXSY    4096 bp ss-mRNA            MAM       25-JAN-1993
DEFINITION  Bovine nitric oxide synthase mRNA, complete cds.
ACCESSION   M99057
KEYWORDS    nitric oxide synthase.
SOURCE      Bos taurus fetal aorta cDNA to mRNA.
  ORGANISM  Bos taurus
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Artiodactyla; Ruminantia; Pecora; Bovidae; Bovinae;
            Bovini.
REFERENCE   1  (bases 1 to 4096)
  AUTHORS   Nishida,K., Harrison,D.G., Navas,J.P., Fisher,A.A., Dockery,S.P.,
            Nerem,R.M., Alexander,R.W. and Murphy,T.J.
  TITLE     Molecular Cloning and Characterization of the constitutive bovine
            aortic Endothelial cell Nitric Oxide synthase
  JOURNAL   J. Clin. Invest. 90, 2092-2096 (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     CDS             30..3647
                     /product="nitric oxide synthase"
                     /codon_start=1
                     /translation="MGNLKSVGQEPGPPCGLGLGLGLGLCGKQGPASPAPEPSRAPAP
                     ATPHAPDHSPAPNSPTLTRPPEGPKFPRVKNWELGSITYDTLCAQSQQDGPCTPRCCL
                     GSLVLPRKLQTRPSPGPPPAEQLLSQARDFINQYYSSIKRSGSQAHEERLQEVEAEVA
                     STGTYHLRESELVFGAKQAWRNAPRCVGRIQWGKLQVFDARDCSSAQEMFTYICNHIK
                     YATNRGNLRSAITVFPQRAPGRGDFRIWNSQLVRYAGYRQQDGSVRGDPANVEITELC
                     IQHGWTPGNGRFDVLPLLLQAPDEAPELFVLPPELVLEVPLEHPTLEWFAALGLRWYA
                     LPAVSNMLLEIGGLEFSAAPFSGWYMSTEIGTRNLCDPHRYNILEDVAVCMDLDTRTT
                     SSLWKDKAAVEINLAVLHSFQLAKVTIVDHHAATVSFMKHLDNEQKARGGCPADWAWI
                     VPPISGSLTPVFHQEMVNYILSPAFRYQPDPWKGSATKGAGITRKKTFKEVANAVKIS
                     ASLMGTLMAKRVKATILYASETGRAQSYAQQLGRLFRKAFDPRVLCMDEYDVVSLEHE
                     ALVLVVTSTFGNGDPPENGESFAAALMEMSGPYNSSPRPEQHKSYKIRFNSVSCSDPL
                     VSSWRRKRKESSNTDSAGALGTLRFCVFGLGSRAYPHFCAFARAVDTRLEELGGERLL
                     QLGQGDELCGQEEAFRGWAKAAFQASCETFCVGEEAKAAAQDIFSPKRSWKRQRYRLS
                     TQAEGLQLLPGLIHVHRRKMFQATVLSVENLQSSKSTRATILVRLDTAGQEGLQYQPG
                     DHIGICPPNRPGLVEALLSRVEDPPPPTESVAVEQLEKGSPGGPPPSWVRDPRLPPCT
                     LRQALTFFLDITSPPSPRLLRLLSTLAEEPSEQQELETLSQDPRRYEEWKWFRCPTLL
                     EVLEQFPSVALPAPLLLTQLPLLQPRYYSVSSAPNAHPGEVHLTVAVLAYRTQDGLGP
                     LHYGVCSTWLSQLKTGDPVPCFIRGAPSFRLPPDPYVPCILVGPGTGIAPFRGFWQER
                     LHDIESKGLQPAPMTLVFGCRCSQLDHLYRDEVQDAQERGVFGRVLTAFSREPDSPKT
                     YVQDILRTELAAEVHRVLCLERGHMFVCGDVTMATSVLQTVQRILATEGDMELDEAGD
                     VIGVLRDQQRYHEDIFGLTLRTQEVTSRIRTQSFSLQERHLRGAVPWAFDPPGPDTPG
                     P"
BASE COUNT      767 a   1406 c   1233 g    690 t
ORIGIN      
        1 gacggaggcc gcccgtccgc cccagcgaca tgggcaactt gaagagtgtg ggccaggagc
       61 ccgggccccc ctgcggcctg gggctggggc tgggcctcgg gctatgcggc aagcagggcc
      121 cagcctcccc ggcacctgag cccagccggg cccccgcacc cgccaccccg cacgcgccag
      181 accacagccc agctcccaac agccccacgc tgacccggcc tccggagggg cccaagttcc
      241 ctcgcgtgaa gaactgggag ctggggagca tcacctacga cactctgtgc gcgcagtccc
      301 aacaggacgg gccctgcact cccaggtgct gcctgggctc cctggtgttg ccccggaaac
      361 tgcagacccg gccctccccg ggacctccac ccgctgagca gctgctgagc caggccaggg
      421 acttcatcaa ccagtactac agctccatca agaggagcgg ctcccaggct cacgaggagc
      481 ggcttcagga ggtggaggcc gaggtggcat ccacgggcac ctaccacctc cgagagagcg
      541 agctggtgtt cggggccaag caggcctggc gcaacgcacc ccgctgcgtg ggccgcatcc
      601 agtgggggaa gctgcaggtg tttgatgccc gggactgcag ctcagcacag gagatgttca
      661 cctacatctg caaccacatc aagtacgcca ccaaccgcgg caaccttcgc tcggccatca
      721 cagtgttccc gcagcgcgcc ccgggccgcg gagacttccg gatctggaac agccagctgg
      781 tgcgctacgc aggctacaga cagcaggatg gctctgtgcg tggggaccca gccaatgtgg
      841 agatcacgga gctctgcatc cagcacggct ggacccccgg aaacggccgc ttcgacgtgc
      901 tgcccctgct gctccaggcc ccagacgagg ctccagagct ctttgttctg ccccccgagc
      961 tggtccttga agtgcccctg gagcacccca cactggagtg gttcgcggcc ctgggcctgc
     1021 gatggtatgc cctcccggcc gtgtccaaca tgctgctgga aatcgggggt ctggagttct
     1081 ccgcggcccc cttcagcggc tggtacatga gcacggagat tggcacgcgg aacctgtgtg
     1141 accctcaccg ctacaatatc ctggaggatg tggccgtctg catggacctc gacacgcgga
     1201 ccacctcgtc cctgtggaag gacaaggcgg ccgtggagat caacctggct gtgctgcaca
     1261 gctttcagct cgccaaggtg accatcgtgg accaccacgc cgccacggtg tccttcatga
     1321 agcacctgga caacgagcag aaggccaggg ggggctgccc cgccgactgg gcctggatcg
     1381 tgccccccat ctcaggcagc ctcacgcccg tcttccacca ggagatggtc aactacatcc
     1441 tgtcccctgc cttccgctac cagccagacc cctggaaagg gagcgcgacc aagggcgcag
     1501 gcatcaccag gaagaagacc tttaaggaag tggccaacgc ggtgaagatc tctgcctcac
     1561 tcatgggcac cctgatggcc aagcgagtga aagcaaccat cctgtacgcc tctgagaccg
     1621 gccgggccca gagctacgct cagcagctgg ggaggctctt ccggaaggcc ttcgatcccc
     1681 gggtcctgtg catggatgag tatgacgtgg tgtccctgga gcacgaggcg ctggtactgg
     1741 tggtgaccag cacctttggg aatggcgatc ccccggagaa tggagagagt tttgcagctg
     1801 ccctgatgga gatgtcgggg ccctacaaca gctccccgcg gccggaacag cacaagagtt
     1861 acaagatccg cttcaacagc gtctcctgct cagacccgct ggtgtcctcc tggcggcgga
     1921 agagaaagga gtccagcaac acagacagtg cgggggccct ggggaccctc aggttctgtg
     1981 tgttcggact gggctcccgg gcataccccc acttctgcgc cttcgcgcga gcggtggaca
     2041 cccggctgga agagcttgga ggggagcggc tgctgcagct gggccagggc gatgagctct
     2101 gcggccagga agaggccttc cgtggttggg caaaggcggc cttccaggcc tcctgtgaga
     2161 ccttctgcgt tggggaggag gccaaggccg ccgcccagga catcttcagc cccaaacgga
     2221 gctggaaacg ccagaggtac cggctgagca cccaggccga gggcctccag ctgctgccag
     2281 gcctgatcca cgtgcacaga cggaagatgt ttcaggccac agtcctctcg gtggaaaatc
     2341 tgcaaagcag caagtccacc cgggccacca tcctggtgcg cctggacact gcaggccagg
     2401 aggggctgca gtaccagccg ggggaccaca taggcatctg cccgcccaac cggccgggcc
     2461 tggtggaggc gctgctgagc cgcgtggagg acccgccacc gcccaccgag tctgtggctg
     2521 tggagcagct ggagaaaggc agcccaggcg gccctcctcc cagctgggtg cgggacccac
     2581 ggctgccccc gtgcacgctg cgccaggctc tcaccttctt cctggacatc acctccccac
     2641 ccagcccccg gcttctccga ctgctcagca ccctggccga agaacccagc gagcagcagg
     2701 agcttgagac cctcagtcag gacccccggc gctacgagga gtggaagtgg ttccgctgcc
     2761 ccacgctgct ggaggtgctg gagcagttcc cgtccgtggc gctgcccgcc ccgctgctcc
     2821 tcacccagct gcccctgctg cagccccggt actactctgt cagctcggcc cccaacgccc
     2881 accccggaga ggtccacctc acagtggccg tgctggcgta caggacccaa gatgggctgg
     2941 gccccctaca ctacggggtc tgctccacat ggctgagcca gctcaagact ggagaccccg
     3001 tgccctgctt catcaggggg gctccctcct tccggctgcc gcctgacccc tacgtgccct
     3061 gcatcctcgt gggccctggc actggcatcg cccccttccg gggattttgg caggagaggc
     3121 tgcatgacat tgagagcaaa gggctgcagc ccgcccccat gaccctggtg ttcggctgcc
     3181 gctgctccca actcgaccat ctctaccgcg acgaggtgca ggacgcccag gagcgcgggg
     3241 tgtttggccg cgtcctcacc gccttctccc gggaacctga cagccccaag acctacgtac
     3301 aggacatcct gagaaccgag ctggctgccg aggtgcaccg cgtgctgtgc ctcgagcggg
     3361 gccacatgtt tgtctgcggc gatgtcacta tggcaaccag cgtcctgcag acggtgcagc
     3421 gcatcttggc gacagagggc gacatggagc tggacgaggc gggcgacgtc atcggcgtgc
     3481 tgcgggatca gcaacgctat cacgaggaca ttttcggcct cacgctgcgc acccaggagg
     3541 tgacaagccg tatacgtacc cagagctttt ccctgcagga gcggcatctg cggggcgcgg
     3601 tgccctgggc cttcgacccg cccggcccag acacccccgg cccctgaaac ccctcttgct
     3661 tcccactgca gttcccggag agaggggctg tcattccact atggctctac cgctgtcctg
     3721 ttggccttta ccgggaccgg ccacctctcc ctcccctccc aaggtgactt cccagagact
     3781 gttggattcc ctgtactatc tcatcctctc atctctaggt ctgtttcccc accctaagtc
     3841 catctggaag acccctccca gcagcggtat tccagagcct acagtcagcc ctttggtgtt
     3901 taggtgaatt ttagattccc ctcgcctctc tccggaagta tcttatcttg aaacctgatc
     3961 tctaaatcat tcaaatattt attattgaag atttaccata agagactgga ccagaagtta
     4021 ggagacctac taagatgcct aagccagtgc tgtcaattac agttacagaa taatgacaaa
     4081 aaaaaaaaaa aaaaaa
//