From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: S.haematobium gene encoding 18S ribosomal RNA.
Date: 12 Sep 92 10:03:37 GMT
Distribution: bionet
Checksum: 21897     6

LOCUS       SCM18SRIBR   1972 bp ds-DNA             INV       12-SEP-1992
DEFINITION  S.haematobium gene encoding 18S ribosomal RNA.
ACCESSION   Z11976
KEYWORDS    18S ribosomal RNA; ribosomal RNA.
SOURCE      Schistosoma haematobium DNA.
  ORGANISM  Schistosoma haematobium
            Eukaryota; Animalia; Eumetazoa; Platyhelminthes; Trematoda;
            Digenea; Strigeidida; Strigeata; Schistosomatoidea;
            Schistosomatidae.
REFERENCE   1  (bases 1 to 1972)
  AUTHORS   Johnston,D.A., Kane,R.A. and Rollinson,D.
  TITLE     Small subunit rRNA gene sequence divergence within the genus
            Schistosoma
  JOURNAL   Unpublished (1992)
  STANDARD  full staff_review
REFERENCE   2  (bases 1 to 1972)
  AUTHORS   Johnston,D.A.
  JOURNAL   Unpublished (1992)
  STANDARD  full staff_review
COMMENT     *source: strain=Mali P1; *source: stage=adult; *source: tissue=
            whole organism - pooled paired male and female *source: worms;
            *source: clone_library=haematobium 3, haematobium RK1; *source:
            clone=pSH20:3, pSHA2/A3/A4/A5/B1/B2/B3/B4; *source: isolate=1730,
            1731, 1732; *source: is_macronuclear=N; *source: is_proviral=N;
            From EMBL    entry SH18SRIBR;  dated 03-SEP-1992.
FEATURES             Location/Qualifiers
     rRNA            <1..>1972
                     /product="18S ribosomal RNA smallsubunit"
                     /gene="18S rRNA"
                     /citation=[1]
                     /citation=[2]
     misc_feature    1..1791
                     /note="Sequence from 5' end of 18S rRNA gene tointernal
                     Hind3 site (position 1786 to 1791)obtained from clone
                     pSH20:3 (Hind3 cut genomic DNAshotgun cloned into PUC19)"
                     /citation=[1]
                     /citation=[2]
     primer_bind     1414..1432
                     /note="PCR primer (designated 24R) used to amplifyportion
                     of gene 3' of the internal Hind3 site."
                     /citation=[1]
                     /citation=[2]
     misc_feature    1792..1972
                     /note="Sequence 3' of internal Hind3 site of 18SrRNA gene
                     (position 1786 to 1791) obtained frompool of 8 clones of
                     PCR-amplified DNA cloned intoPCR1000
                     (pSHA2/A3/A4/A5/B1/B2/B3/B4)"
                     /citation=[1]
                     /citation=[2]
     primer_bind     complement(1951..1972)
                     /note="PCR primer (designated AW13) used toamplify portion
                     of gene 3' of the internal Hind3site. NB sequence of
                     actual gene may be differentto primer."
                     /citation=[1]
                     /citation=[2]
BASE COUNT      473 a    415 c    560 g    524 t
ORIGIN
        1 aaactggttg atcctgccag tagtcatatg cttgtctcag agattaagcc atgcatgtct
       61 aagtacatac cttaaaacgg tgaaaccgcg aatggctcat taaatcagct atggttcctt
      121 agatcgtaaa tgctacatgg ataactgtag taattctaga gctaatacat gccttgaatc
      181 cctgacccgc aagggaacgg gtgcatttat tagaacagaa ccaatcgggc gcggcttcgg
      241 ctgtgcctgc tacattctgt gatgactctg gataacttta ctgatcgcag tcggccttgt
      301 gtcggcgacg gatctttcaa atgtctgccc tatcaatttg ttggtaggtg atttgcctac
      361 catgatgata acgggtaacg gggaatcagg gttcgattcc ggagagggag cctgagaaat
      421 ggctaccaca tccaaggacg gcagcaggcg cgaaaattac ccactcccgg cacggggagg
      481 tagtgacgaa aaatacggat acgggactca attgaggctc cgtaattcga atgagtacaa
      541 tttaaatcct ttaacgagga ccaattggag ggcaagtctg gtgccagcag ccgcggtaat
      601 tccagctcca aaagcgtata ttaaagttgc tgcagttaaa aagctcgtag ttggatctgg
      661 gtcgtgcggt cgcatgccgc tgcttgttca cggttttggt tacgatcagg acgtgttcag
      721 ctcggtgtag tggctgtgca gcctttcagc cgtgtctgtg ttaaacgggt gctggtgggt
      781 tgacgagttc gtcttgttga cctgtcggca tgcttccgga tgcctttaaa cgggtgtcgg
      841 gagcggacgg catctttact ttgaacaaat ttgagtgctc aaagcaggcc tgtgcgcctg
      901 aaaattcttg catggaataa tgaaatagga cttcggttct attttgttgg ttttcggatc
      961 cgaagtaatg gttaagaggg acagacgggg gcatttgtat ggcggtgtta gaggtgaaat
     1021 tctgggatcg ccgccagaca aactacagcg aaagcatttg ccaagaatgt tttcattgat
     1081 caggagcgaa agtcagagtt tcgaagacga tcagataccg tcgtagttct gaccataaac
     1141 aatgccgact gatgatccgc gttggttcta taattgacat cgcaggcagt ccccgggaaa
     1201 cctttaagtc tttgggctcc ggggggagta tggttgcaaa gctgaaactt aaaggaattg
     1261 acggaagggc accaccagga gtggagcctg cggtttaatt cgactcaaca cgggaaaact
     1321 cacccggccc ggacactgtg aggattgaca gattgatagc tctttcttga ttcggtgggt
     1381 ggtggtgcat ggccgttctt agttggtgga gcgatttgtc tggttaattc cgataacgaa
     1441 cgagacttta acctgctaaa tagtagactg gtcctctgtg ctcgtttagg gcgcggcttc
     1501 tattgcttct ttatggagta gtgtggtcgt gatccggcgg gtgcggtgcc agtttttact
     1561 tcttagaggg acaagcggca cacttaagtc gcacgaaatt gagcaataac aggtctgtga
     1621 tgcccttaga tgtccggggc cacacgtgcg ctacaatgac ggtgccagcg agtctgggaa
     1681 cctggcccga aagggttggg caaactgttt catcaccgtc gtgactggga tcggggcttg
     1741 caattattcc ccgtgaacga ggaattcctg gtaagtgcaa gtcataagct tgcgctgatt
     1801 acgtccctgc cctttgtaca caccgcccgt cgctactacc gattgaatgg tttagtgagg
     1861 tcgttggatt ggtgtcgttg tagtggcgtt gccgctcgac tgatgctgag aagatgacct
     1921 aatttgacta tttagaggaa gtaaaagtcg taacaaggtt tccgtaggtg aa
//