From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Plasmid pHH507 (from E. coli) replication protein A (repA) gene,
Date: 10 Sep 92 10:31:55 GMT
Distribution: bionet
Checksum: 24363     4

LOCUS       H50REPA      1525 bp ds-DNA             BCT       10-SEP-1992
DEFINITION  Plasmid pHH507 (from E. coli) replication protein A (repA) gene,
            complete cds.
ACCESSION   L01252
KEYWORDS    repA gene; replication protein A.
SOURCE      Plasmid pHH507 DNA.
  ORGANISM  Plasmid pHH507
            Prokaryotae.
REFERENCE   1  (bases 1 to 1525)
  AUTHORS   Gibbs,M.D., Spiers,A.J. and Bergquist,P.L.
  TITLE     RepFIB: A basic replicon of large plasmids
  JOURNAL   Unpublished (1992)
  STANDARD  full staff_entry
FEATURES             Location/Qualifiers
     CDS             276..1253
                     /note="central protein required for replication"
                     /product="replication protein A"
                     /gene="repA"
                     /codon_start=1
                     /translation="MDKSSGELVTLTPNNNNTVQPVALMRLGVFVPTLKSLKNSKKNT
                     LSRTDATEELTRLSLARAEGFDKVEITGPRLDMDNDFKTWVGIIHSFARHNVIGDKVE
                     LPFVEFAKLCGIPSSQSSRRLRERISPSLKRIAGTVISFSRTDEKHTREYITHLVQSA
                     YYDTERDIVQLQADPRLFELYQFDRKVLLQLKAINALKRRESAQALYTFIESLPRDPA
                     PISLARLRARLNLKSPVFSQNQTVRRAMEQLREIGYLDYTEIQRGRTKLFCIHYRRPR
                     LKAPNDESKENPLPPSPAEKVSPEMAEKLALLEKLGITLDDLEKLFKSR"
BASE COUNT      384 a    380 c    367 g    394 t
ORIGIN
        1 gggccgggac cacgttcaca taagcggtgg catgttatgg ataaactgta acaggttcat
       61 gataagctgt attcagtaat gcatatactg aagtaagtta atgacataaa ctatggtcag
      121 tacgccagac tcagttgtta aatacagact gcaggttttt cttcagtcag ttgacggggt
      181 tctgacacac gattttctgt ttattctttt actgtccaca ggcaggaggc tttctggaaa
      241 acgaaaattc agacatcaaa aaactgttcg gcgaggtgga taagtcgtcc ggtgagctgg
      301 tgacactgac accaaacaat aacaacaccg tacaacctgt ggcgctgatg cgtctgggcg
      361 tttttgtacc gacccttaaa tcactgaaga acagtaaaaa aaatacactg tcacgtactg
      421 atgccacgga agagctgaca cgtctttccc tggcccgtgc tgagggattc gataaggttg
      481 agatcactgg cccccgcctg gatatggata acgatttcaa gacctgggtg gggatcattc
      541 attcctttgc ccgccataac gtgattggtg acaaagttga actgcctttt gttgagtttg
      601 caaaactgtg tggtatacct tcaagccagt catcccgcag gctgcgtgag cgcatcagcc
      661 cttccctgaa gcgcattgcc ggtaccgtga tctcgttttc ccgtaccgat gagaagcaca
      721 cccgggaata catcacccat ctggtacagt cagcctacta cgatactgaa cgggatattg
      781 ttcagttaca ggctgatccc cgcctttttg aactgtacca gtttgacaga aaggtccttc
      841 ttcagcttaa ggcgattaat gccctgaagc gacgggagtc cgcccaggca ctttacacct
      901 ttatagagag cctgccccgg gatccggcac cgatatcgct ggcacggctg cgtgcacgcc
      961 tcaatctgaa gtctcctgta ttttcccaga accagacggt cagacgggca atggagcagt
     1021 tgcgcgagat tggatatctt gattacacgg agatccagcg ggggcggaca aaactcttct
     1081 gcattcacta ccggcgtccc cggttaaaag caccgaatga tgagagtaag gaaaatccgt
     1141 tgccaccttc acctgcggaa aaagtcagtc cggagatggc ggagaagctt gccctgcttg
     1201 aaaaactggg catcacgctg gatgacctgg aaaaactctt caaatcccgc tgaacataaa
     1261 ctgtagtcag tgaagagtgc tcctttactg actacagctt atattatcag gtgcagtgag
     1321 tggtctgctc actgcagttt atattcagtt tcctgcagtg ctgcctgtgg ctgagctgcc
     1381 atctgcctgt cccttacgtg agtcaccccg taacctgatg ctgaggcatt tctcccttta
     1441 taaaacatgg tttactcact acagcttatc tacctgctcc agcttatgtt atgtctgttc
     1501 tgctgaccac tgcttatata aggaa
//