From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Escherichia coli dapE gene for succinyldiaminopimelate
Date: 15 Sep 92 10:02:53 GMT
Distribution: bionet
Checksum: 58896     6

LOCUS       ECODAPE      2270 bp ds-DNA             BCT       15-SEP-1992
DEFINITION  Escherichia coli dapE gene for succinyldiaminopimelate
            desuccinylase
ACCESSION   X57403
KEYWORDS    dapE gene; succinyldiaminopimelate desuccinylase.
SOURCE      Escherichia coli DNA.
  ORGANISM  Escherichia coli
            Prokaryotae; Gram-negative facultatively anaerobic rods;
            Enterobacteriaceae.
REFERENCE   1  (bases 1 to 2270)
  AUTHORS   Karmazyn-Campelli,C., Bonamy,C., Savelli,B. and Stragier,P.
  TITLE     Tandem genes encoding sigma-factors for consecutive steps of
            development in Bacillus subtilis
  JOURNAL   Genes Dev. 3, 150-157 (1989)
  STANDARD  simple staff_entry
REFERENCE   2  (sites)
  AUTHORS   Bouvier,J., Richaud,C., Higgins,W., Boegler,O. and Stragier,P.
  TITLE     cloning, characterization, and expression of the dapE gene od
            Escherichia coli
  JOURNAL   J. Bacteriol. 174, 5265-5271 (1992)
  STANDARD  full staff_review
COMMENT     *source: strain=K12;
            From EMBL    entry ECDAPE;  dated 06-SEP-1991.
FEATURES             Location/Qualifiers
     CDS             <1..124
                     /note="3' end of upstream orf"
                     /codon_start=2
                     /translation="HAVGTGVMGGMISATILAIYFVPLFFVLVRRRFPLKPRPE"
     CDS             663..1018
                     /note="orf"
                     /codon_start=1
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLND
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCVPGKPM
                     LLGFSDSSYQQFFHEV"
                     /partial
     CDS             1023..2149
                     /EC_number="3.5.1.18"
                     /product="succinyldiaminopimelate desuccinylase"
                     /gene="dapE"
                     /codon_start=1
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
                     /partial
     CDS             2178..>2270
                     /note="5' end of downstream orf"
                     /codon_start=1
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVD"
BASE COUNT      565 a    511 c    569 g    625 t
ORIGIN
        1 gcatgcggtg ggtactggcg taatgggcgg gatgatttcg gccactattc tggctattta
       61 cttcgtgccg ctgttctttg tgctggtgcg ccgccgcttc ccgctgaagc cgcgcccgga
      121 ataagcaata aaaaaggcga catgccaatg tgtcgccttt ttcaactttc cgattaagaa
      181 cctgctcagc gggttcttgc tgtttgtact ttgtctcagg aattacttac gaagcataac
      241 ttcgataaag tctttccagt tccccagttc acgttcaatc ataacaacct ctcttataat
      301 tatgggtatt ctacggaaac aatataccgt ggtgaagcta atttactcga ttgctgcgat
      361 gactacctcc gggggacaaa tcttatgtaa atactatggt cctacagtaa taatttgtat
      421 gtaatacaca gcaacatttc gagatattca tacggcatct aatactgatt taattctggt
      481 taaaatacag acagataaca agatgaatat tcttaatgtt tacgttaaaa atgtttaata
      541 ttatttaata gttgttaatt tgaatacttc gataatgtta tatttcctga taatcatttg
      601 caggcaaaat gttttcaccc ttaaatgagt atttattctc ataaatcgaa aaaggattca
      661 ttatggttac actttacggt atcaaaaatt gtgacaccat taaaaaggct cgccgttggc
      721 tggaagccaa taacatcgac tatcgttttc atgattaccg cgtcgatggg ctggacagcg
      781 aattattgaa cgattttatc aacgaattag gctgggaagc gttactcaac acccgtggta
      841 caacctggcg taaactggac gaaaccaccc gcaataaaat caccgatgcg gcctctgcgg
      901 cggcattaat gactgaaatg cctgcaatta tcaaacgtcc attgctctgc gtgcccggta
      961 agcctatgct gctgggtttc agtgattcca gttatcagca atttttccat gaggtgtagt
     1021 ctatgtcgtg cccggttatt gagctgacac aacagcttat tcgccgccct tccctgagtc
     1081 ctgatgatgc aggatgccag gctttgttga ttgaacgttt gcaggcgatc ggttttaccg
     1141 ttgaacgcat ggactttgcc gatacgcaga atttttgggc atggcgtggg cagggtgaaa
     1201 cgttagcctt tgccgggcat accgacgtgg tgccgcctgg cgacgccgat cgttggatca
     1261 atcccccgtt tgaacccacc attcgtgacg gcatgttatt cgggcgcggt gcggcagata
     1321 tgaaaggctc gctggcggcg atggtggtgg cggcagaacg ttttgtcgca caacatccca
     1381 accatacggg gcgactggca tttctgatca cctctgatga agaagccagt gcccacaacg
     1441 gtacggtaaa agtcgtcgaa gcgttaatgg cacgtaatga gcgtctcgat tactgcctgg
     1501 ttggcgaacc gtcgagtatc gaagtggtag gtgatgtggt gaaaaatggt cgtcgcggat
     1561 cattaacctg caaccttacc attcatggcg ttcaggggca tgttgcctac ccacatctgg
     1621 ctgacaatcc ggtacatcgc gcagcacctt tccttaatga attagtggct attgagtggg
     1681 atcagggcaa tgaattcttc ccggcgacca gtatgcagat tgccaatatt caggcgggaa
     1741 cgggcagtaa caacgttatt ccgggtgaac tgtttgtgca gtttaacttc cgcttcagca
     1801 ccgaactgac tgatgagatg atcaaagcgc aggtgcttgc cctgcttgaa aaacatcaac
     1861 tgcgctatac ggtggattgg tggctttccg ggcagccatt tttgaccgcg cgcggtaaac
     1921 tggtggatgc ggtcgttaac gcggttgagc actataatga aattaaaccg cagctactga
     1981 ccacaggcgg aacgtccgac gggcgcttta ttgcccgcat gggggcgcag gtggtggaac
     2041 tcgggccggt caatgccact attcataaaa ttaatgaatg tgtgaacgct gccgacctgc
     2101 agctacttgc ccgtatgtat caacgtatca tggaacagct cgtcgcctga tgagtggttc
     2161 tgcaagagga aataagcatg gactggctgg ctaaatattg gtggattctg gtgattgtct
     2221 ttttggtagg cgtcctgctg aacgtgatta aagatctcaa gcgcgtcgac
//