From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Saccharomyces cerevisiae ubiquitin-specific processing protease
Date: 26 Jan 93 05:53:55 GMT
Distribution: bionet
Checksum: 10424 11

LOCUS       YSCUBPII     6008 bp ds-DNA             PLN       25-JAN-1993
DEFINITION  Saccharomyces cerevisiae ubiquitin-specific processing protease
            (Ubp2) gene, complete cds.
ACCESSION   M94916
KEYWORDS    ubiquitin-specific processing protease.
SOURCE      Saccharomyces cerevisiae (strain GRF88) DNA.
  ORGANISM  Saccharomyces cerevisiae
            Eukaryota; Fungi; Eumycota; Hemiascomycetes; Endomycetales;
            Saccharomycetaceae.
REFERENCE   1  (bases 1 to 6008)
  AUTHORS   Baker,R.T., Tobias,J.W. and Varshavsky,A.
  TITLE     Ubiquitin-specific proteases of Saccharomyces cerevisiae: Cloning
            of UBP2 and UBP3, and functional analysis of the UBP gene family
  JOURNAL   J. Biol. Chem. 267, 23364-23375 (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     CDS             983..4777
                     /gene="Ubp2"
                     /product="ubiquitin-specific processing protease"
                     /codon_start=1
                     /translation="MPNEDNELQKAIENHHNQLLNQDKENADRNGSVIEDLPLYGTSI
                     NQQSTPGDVDDGKHLLYPDIATNLPLKTSDRLLDDILCDTIFLNSTDPKVMQKGLQSR
                     GILKESMLSYSTFRSSIRPNCLGSLTDQVVFQTKSEYDSISCPKYNKIHVFQAVIFNP
                     SLAEQQISTFDDIVKIPIYHLKVSVKVRQELERLKKHVGVTQFHSLDHLHEYDRVDLS
                     TFDSSDPNLLDYGIYVSDDTNKLILIEIFKPEFNSPEEHESFTADAIKKRYNAMCVKN
                     ESLDKSETPSQVDCFYTLFKIFKGPLTRKSKAEPTKTIDSGNLALNTHLNPEWLTSKY
                     GFQASSEIDEETNEIFTEYVPPDMVDYVNDLETRKIRESFVRKCLQLIFWGQLSTSLL
                     APNSPLKNTKSVKGMSSLQTSFSTLPWFHLLGESRARILLNSNEQTHSPLDAEPHFIN
                     LSVSHYYTDRDIIRNYESLSSLDPENIGLYFDALTYIANRKGAYQLIAYCGKQDIIGQ
                     EALENALLMFKINPKECNISELNEATLLSIYKYETSNKSQVTSNHLTNLKNALRLLAK
                     YTKSDKLKFYVDHEPYRALSQAYDTLSIDESVDEDIIKTAYSVKINDSPGLKLDCDRA
                     LYTIAISKRSLDLFNFLTEECPQFSNYYGPEKLLQVNENASDETILKIFKQKWFDENV
                     YEPDQFLILRAALTKISIERNSTLITNFLLTGTIDPNSLPPENWPTGINNIGNTCYLN
                     SLLQYYFSIAPLRRYVLEYQKTVENFNDHLSNSGHIRRIGGREISRGEVERSIQFIYQ
                     LRNLFYAMVHTRERCVTPSKELAYLAFAPSNVEVEFEVEGNKVVDQTGVLSDSKKETT
                     DDAFTTKIKDTSLIDLEMEDGLNGDVGTDANRKKNESNDAEVSENEDTTGLTSPTRVA
                     KISSDQLENALEMGRQQDVTECIGNVLFQIESGSEPIRYDEDNEQYDLVKQLFYGTTK
                     QSIVPLSATNKVRTKVERFLSLLINIGDHPKDIYDAFDSYFKDEYLTMEEYGDVIRTV
                     AVTTFPTILQVQIQRVYYDRERLMPFKSIEPLPFKEVIYMDRYADTENPLLLAKKKET
                     EEMKQKLKVMKNRQRELLSRDDSGLTRKDAFLESIKLLESDTIKKTPLKIEAANDVIK
                     TLRNNVQNIDNELMKLYNDINSLEEKISHQFDDFKEYGYSLFSVFIHRGEASYGHYWI
                     YIKDRNRNGIWRKYNDETISEVQEEEVFNFNEGNTATPYFLVYVKQGQEGDIEPLKRI
                     LK"
BASE COUNT     2127 a   1035 c   1211 g   1635 t
ORIGIN      
        1 gcatgctccc aagtgtcaga atttatcaga tgctcaggct gcatttttgg accgtgttat
       61 tcgtgtagat caagctggcg aattaggtgc agactacatc tacgctggcc agtacttcgt
      121 gttggctcat aggtaccctc acttgaaacc tgtgctaaag cacatatggg accaggagat
      181 acatcatcat aatactttta acaatttgca attgaaaagg agagtcaggc cttccttatt
      241 aacgcctttg tggaaggcag gagcctttgc aatgggggct ggtaccgcat tgatttctcc
      301 agaagcagct atggcttgta ctgaagctgt cgagacagta atcggagggc actacaatgg
      361 ccaattgcga aacttggcca atcaattcaa tttagaaaga acagatggaa caaagggtcc
      421 aagtgaggaa atcaaatcct taacttctac tatccaacag ttcagggatg acgagctaga
      481 gcatctagac accgctatca agcatgattc gtatatggca gttccatata cagttatcac
      541 tgaaggtatt aaaacgattt gcagagtagc tatatggagt gccgaaagaa tttaaccacc
      601 agaaagtggc atacatcagt cgcgttatgc cagaaaagga gaattgaaag gaaaacggtt
      661 tgataaatgt cctaattaaa ctatcatgta taaaattatg tatcatcctt acgcatttta
      721 acgctatatg accaatatga caggaataga tacactgtct ataattatgt aaatggggta
      781 tgggttcata gtctaagggt gagtacaaac tggatcttta acaagagtaa cagttaatta
      841 gagcaaaact atagtacata tagcttgaaa aaaacaagcg gcttgccatt ggaagaacat
      901 tgcataaaaa cggggccact gctaataata aagtggtaat taaaaagaaa gcttttgttc
      961 aaggttaaga aggtataagg aaatgccgaa cgaagataat gaacttcaaa aagcaattga
     1021 gaaccatcat aatcaactac taaaccagga taaagaaaat gctgacagaa atgggtctgt
     1081 tatagaagac ctcccattat acgggacaag tataaaccag cagtctaccc ctggagatgt
     1141 tgacgatgga aaacacttac tgtatccaga tattgccacc aacctaccac tgaagacttc
     1201 tgacagactt ttggacgata tactttgcga tactattttt ctcaattcta cagacccgaa
     1261 ggtcatgcaa aagggcctgc aatcgagggg tattttaaaa gagtctatgc tttcttactc
     1321 aactttcaga agtagtattc gccctaactg cttgggttca ttaactgatc aagtggtttt
     1381 tcaaacaaaa tccgagtatg attccatttc atgcccaaaa tataataaaa tacatgtatt
     1441 tcaggcggtc atctttaatc catcactggc agaacagcaa atttcaactt ttgatgatat
     1501 tgttaaaatt cctatttatc atcttaaggt tagcgtaaaa gtccgccaag aactggagcg
     1561 gttgaagaag catgtcggtg ttactcaatt ccactcacta gatcatttgc acgaatacga
     1621 tcgagtagac ctttcgactt ttgattcttc cgatcctaat ttgttggatt acggtattta
     1681 cgtttctgat gatactaaca aactgatctt gattgaaatt tttaaacccg agtttaattc
     1741 acctgaagag catgagagtt ttactgccga cgcaattaag aagagataca atgctatgtg
     1801 tgtaaaaaat gaatcactag ataaaagcga gacgccatct caagttgact gtttttacac
     1861 actttttaaa atttttaaag ggcctttgac gaggaaaagt aaagcggaac ctacaaagac
     1921 aattgattct ggaaatttgg cccttaacac tcacctgaat cctgaatggt taacgtccaa
     1981 gtatggattt caagcaagct cagaaatcga tgaggaaact aatgagatat ttactgaata
     2041 cgtccctcca gatatggtgg actatgtaaa cgatttggag acaagaaaaa ttcgagaatc
     2101 gtttgtgagg aagtgtttac aactgatatt ttggggtcaa ctatctacct cattactggc
     2161 acctaattct cccttgaaaa atacgaaaag cgtaaaggga atgtcttcat tacaaacttc
     2221 tttctcaaca ctaccttggt tccatttatt gggagaatcc agagcaagga ttctattaaa
     2281 ttccaatgag caaactcatt ctcctttgga cgcagaacct cattttatta atctttccgt
     2341 ttcgcattat tataccgata gagatataat cagaaactac gaatctttgt cttctttgga
     2401 tcctgaaaat attgggctgt attttgacgc actgacatac attgcaaata ggaagggggc
     2461 atatcaattg attgcttact gtggaaaaca ggacattata ggccaagaag ctctagaaaa
     2521 tgctttgtta atgtttaaaa ttaaccctaa agagtgtaac atctccgaat taaatgaggc
     2581 gactttgcta tctatttaca aatatgaaac atcaaataag agccaagtaa cctctaatca
     2641 cctaacaaat ttgaaaaatg ctctaagatt gttggccaaa tataccaaat ctgacaaact
     2701 aaaattttac gtcgatcatg agccctacag agctttatcc caggcatacg acacactttc
     2761 aattgacgag tctgttgatg aagacattat aaaaactgca tattcggtca agattaacga
     2821 ctctcccgga ttaaagttgg attgtgatag agcactttac accattgcta tcagtaaaag
     2881 aagccttgat ttgttcaatt ttttaacaga ggaatgccca cagttttcca actattatgg
     2941 tccagagaag cttcttcaag tgaatgaaaa tgcctctgac gaaaccattt tgaaaatctt
     3001 taaacaaaag tggtttgatg aaaacgttta tgagcctgac caatttctta ttttgagggc
     3061 agcattgacc aaaatcagta tagaaagaaa ttcaacttta atcaccaact tcttactaac
     3121 tggtacgata gatccaaatt ccttgccgcc agaaaattgg ccaactggca ttaataatat
     3181 cgggaacacc tgttacctaa attctttatt acaatattac ttttccattg cgccactaag
     3241 aagatatgta ttggaatatc aaaaaacggt agaaaatttc aatgaccacc tctctaatag
     3301 tgggcatatt agaagaattg gtggaagaga aattagtaga ggcgaagtgg aaagatctat
     3361 tcaattcata taccaacttc gcaacctttt ctatgcgatg gttcatacaa gagaaagatg
     3421 tgtaacaccc tcaaaagagc tagcatattt ggcatttgct ccaagtaatg ttgaagtaga
     3481 atttgaagtg gaaggcaata aagtagttga tcaaacagga gttctttcgg attcaaagaa
     3541 ggaaacaacg gatgacgcat ttactacaaa aataaaggat acaagcctga ttgatttaga
     3601 aatggaagat ggccttaatg gcgatgttgg tacagatgcg aacagaaaaa aaaatgaatc
     3661 gaatgatgct gaagtaagtg agaacgaaga tacaacagga ttaacttcac ctacgcgtgt
     3721 ggcaaaaatc agttctgatc aattagaaaa tgctttggaa atgggtaggc aacaagatgt
     3781 tactgaatgc ataggaaacg tgttatttca gatagaaagc ggttcagagc ctatccgata
     3841 tgatgaagac aacgagcaat atgacttggt taagcaacta ttttatggta ctactaaaca
     3901 aagtattgtt cctttgtccg caacaaataa agtccgtacg aaagttgaaa gattcctatc
     3961 gttactgata aatattggcg atcatcctaa agatatttat gatgcgtttg attcttattt
     4021 taaagacgaa tatctgacaa tggaagagta tggtgatgtt atacgtaccg ttgctgttac
     4081 aacttttcct actattttgc aggtacaaat ccaaagagtt tattacgatc gtgaaagatt
     4141 aatgccgttt aaatccattg agcccttacc attcaaagaa gttatttaca tggacagata
     4201 cgcggataca gagaaccctt tattgttggc aaaaaagaaa gaaacagaag aaatgaagca
     4261 aaagttgaag gtaatgaaaa atagacaaag agagcttttg agtcgtgatg attcagggct
     4321 tacaaggaag gatgcatttt tggagagtat caagctattg gaatcggata ccataaagaa
     4381 aactccttta aaaattgagg ctgctaatga tgtgataaag acgctgagaa acaacgttca
     4441 aaatatcgat aatgaattga tgaaattata caatgatatc aacagtttgg aagagaaaat
     4501 aagccatcaa tttgacgatt tcaaggaata tggttactca ctgttttcgg tttttattca
     4561 tcgcggcgag gccagttatg gtcactattg gatatatatc aaggacagaa atcgcaatgg
     4621 aatttggagg aagtacaatg atgaaaccat cagcgaggtc caggaagagg aggtcttcaa
     4681 tttcaatgag ggtaacactg caactccata tttcctagta tatgtcaaac aaggacaaga
     4741 aggtgatatt gagccattga aaagaattct aaagtagtct tagtcaatga agagtttatg
     4801 taaaatgtca ctattgccat aagtaccatt attatgtaaa aagctttgcc atattcaatg
     4861 ttacgggtga ctatctgcta cgtaaagaaa aacgaaaaaa caaaaaaaaa aagaacaagc
     4921 tcatagaagt gaatacgaaa gctgaagaaa gtcgttaagt agataggttg cgtaaactag
     4981 gtgcgtccaa tcaaagtaat ccaattagat atactggact ataattaaga tgtcatctga
     5041 aagcccacag gatcaaccac agaaggagca aatcagcaat aacgtcggcg ttaccaccaa
     5101 tagtacaagc aatgaggaaa caagccgctc tcaagatgat aatgtcaagg aagtcaatgg
     5161 aaatgatgat actaaagaag aggaacaaga agaagacgca gaactagatg atttatttgg
     5221 agatgacaat gatgacgatg atgatgatga tgttaaaaaa tcggagactg aaaaaagtga
     5281 tagtgatagt gatgaagacg acgagggaga gaatatcaac catagaagtc gtcatagaga
     5341 aagtctcggg ttagatgatg atgaagcaga ggagcaagcc atgtacaccc gaaaatttta
     5401 tggtgaggat gctaataact tttctgatct tgatgagact actcacactt ttaaagagga
     5461 aaatgtagag cttgtcagac atattattcc aagtaaagct aatgtgaatg aaacggcgtc
     5521 tcacaacgaa attttctatg ctagaattcc caacttttta actatcgatc caattccttt
     5581 cgaccctcca agttttgagg ccaaagtaaa cgaaagggca agcaattcag cttctaggga
     5641 ggatcaactg gacgaccgcc tgattgatga aaacactgtt agatggagat actctcgtga
     5701 caaagaccaa catgtcttta aagaatcaaa tacacaaata gtgcagtggt cagacggtac
     5761 atattcgcta aaagttggtg aagagtgtac agatatattg gtcaacgata cgagcaacac
     5821 ttttttgaca gtatcgcatg accaacaaga gttgatccag tgttacgaag ggggtgaaat
     5881 aaaaaagacg ttgatgttta ttccaacttc gacgaattca aaaatacatc aaaaactaag
     5941 taaagctgtt ataagaagga accaaagaca aagcaagggt cctggaaata cattgtaagt
     6001 atggatcc
//