From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: N. sylvestris tufB gene for chloroplast elongation factor TuB
Date: 12 Jan 93 05:51:19 GMT
Distribution: bionet
Checksum: 54596 4

LOCUS       TOBTUFB      1436 bp ss-mRNA            PLN       12-JAN-1993
DEFINITION  N. sylvestris tufB gene for chloroplast elongation factor TuB
            (EF-TuB), partial sequence.
ACCESSION   D11376
KEYWORDS    EF-TuB; G-binding protein;
            nuclear-encoded chloroplast elongation factor TuB.
SOURCE      Nicotiana sylvestris young leaf cDNA to mRNA.
  ORGANISM  Nicotiana sylvestris
            Eukaryota; Plantae; Embryobionta; Magnoliophyta; Magnoliopsida;
            Asteridae; Solanales; Solanaceae.
REFERENCE   1  (bases 1 to 1436)
  AUTHORS   Murayama,Y., Matsubayashi,T., Sugita,M. and Sugiura,M.
  TITLE     Purification of tobacco chloroplast EF-Tu and analysis of its cDNAs
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
COMMENT     Submitted (19-Jun-1992) to DDBJ by: Masahiro Sugiura Center for
            Gene Research Nagoya University Chikusa, Nagoya 464-01 Japan Phone:
             052-781-5111 x3449 Fax:    052-782-3849.
FEATURES             Location/Qualifiers
     transit_peptide <1..51
     mat_peptide     52..1275
                     /product="chloroplast elongation factor TuB (EF-TuB)"
                     /codon_start=1
     CDS             <1..1278
                     /gene="tufB"
                     /product="chloroplast elongation factor TuB (EF-TuB)"
                     /codon_start=1
                     /translation="PTTTPSTTHPRRFTVRAARGKFERKKPHVNIGTIGHVDHGKTTL
                     TAALTMALASMGNSAPKKYDEIDAAPEERARGITINTATVEYETENRHYAHVDCPGHA
                     DYVKNMITGAAQMDGAILVVSGADGPMPQTKEHILLAKQVGVPNMVVFLNKQDQVDDE
                     ELLELVELEVRELLSSYEFPGDEIPIISGSALLALEALMANPSIKRGENQWVDKIYQL
                     MDNVDEYIPIPQRQTELPFLMAIEDVFSITGRGTVATGRVERGTVKVGEIVDIVGLKD
                     TRNTTVTGVEMFQKILDEAMAGDNVGLLLRGIQKIDIQRGMVLAKPGTITPHTKFEAL
                     VYVLKKEEGGRHSPFFAGYRPQFYMRTTDVTGKVTVIMSDKGEESKMVMPGDRVNMVV
                     ELIMPVACEQGMRFAIREGGKTVGAGVIQKILE"
BASE COUNT      400 a    251 c    374 g    411 t
ORIGIN      
        1 ccaacaacta ctccttccac cacccacccc cgtcggttca ctgtccgcgc tgcacgtggc
       61 aaattcgagc gtaaaaaacc tcacgtcaac attggtacaa ttggccacgt tgaccatgga
      121 aagaccacac tcacagctgc tttgaccatg gcgcttgcct ctatgggcaa ctccgccccc
      181 aagaaatatg acgaaattga tgctgcccct gaagaaaggg cgcgtggtat tactatcaac
      241 actgccactg tggaatatga aacggaaaac agacattatg cacacgtgga ctgcccgggg
      301 catgctgatt atgtcaagaa catgattact ggtgctgccc aaatggatgg ggcaattctt
      361 gttgtgtcag gtgctgatgg cccaatgcca cagactaaag agcatatttt gttagctaag
      421 caagttgggg tccctaatat ggttgttttc ttgaacaaac aagaccaagt tgatgatgag
      481 gagttacttg agcttgttga gttggaggta agagaattat tgtcaagtta tgagttccct
      541 ggtgatgaaa ttcctattat ttctggttct gcacttttag ctttagaggc tttgatggct
      601 aatcctagta ttaaaagggg tgaaaatcaa tgggttgata agatttatca attgatggat
      661 aatgttgatg aatatatccc tatcccacaa agacaaactg aattgccttt cttgatggct
      721 attgaggatg ttttctcgat taccggtaga ggtactgtgg cgacggggag agtagagaga
      781 gggactgtta aggttgggga aattgttgat atagttggat tgaaggatac taggaatact
      841 acagtgacag gggttgagat gtttcagaag attttggatg aagcgatggc gggagataat
      901 gtgggattgt tgttgagagg tattcagaag attgatattc agagagggat ggtgttggcg
      961 aagcccggaa caattactcc gcacacaaag tttgaagctt tggtgtatgt gttgaagaag
     1021 gaagagggag gaaggcattc cccgttcttt gcgggttata ggcctcaatt ttacatgagg
     1081 acaactgatg tgactggaaa ggttactgtg attatgagtg acaaaggaga ggaatctaag
     1141 atggtcatgc ctggcgatcg tgtaaacatg gtggttgagc ttatcatgcc ggttgcatgt
     1201 gagcaaggga tgaggtttgc tatcagggaa ggaggaaaga ctgttggagc tggtgttatt
     1261 cagaaaatct tagaatgatg aacttgcagc tgagcatctc ttttcacatg atcggcactt
     1321 tccattgaag ttacttaatc cattgtcata tatgcaactt cttggttact tttattatgt
     1381 cttagaatct tactttagta gaagtatcct gttttaaaca ccaaattcta ctgaac
//