From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Rattus norvegicus lymphotoxin (TNF-beta) gene, complete cds, tumor
Date: 5 Nov 92 23:12:57 GMT
Distribution: bionet
Checksum: 28143 15

LOCUS       RATLYMTNF    7105 bp ds-DNA             ROD       08-OCT-1992
DEFINITION  Rattus norvegicus lymphotoxin (TNF-beta) gene, complete cds, tumor
            necrosis factor (TNF-alpha) gene, complete cds.
ACCESSION   L00981
KEYWORDS    lymphotoxin; tumor necrosis factor.
SOURCE      Rattus norvegicus (strain Sprague-Dawley) (library: lambda DASH II
            from Stratagene) male testis DNA.
  ORGANISM  Rattus norvegicus
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Rodentia; Myomorpha; Muridae; Murinae.
REFERENCE   1  (bases 1 to 7105)
  AUTHORS   Kwon,J., Chung,I.Y. and Benveniste,E.N.
  TITLE     Cloning and structural analysis of the rat TNF locus, including the
            TNF-alpha (tumor necrosis factor) and TNF-beta (lymphotoxin) genes
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     mRNA            join(4432..4617,5098..5152,5301..5348,5631..6858)
                     /gene="TNF-alpha"
     5'UTR           join(1140..1281,1634..1642)
     mat_peptide     join(1643..1738,1821..1920,2142..2551)
                     /product="lymphotoxin"
                     /gene="TNF-beta"
                     /codon_start=1
     mRNA            join(1140..1281,1634..1738,1821..1920,2142..3135)
                     /gene="TNF-beta"
     misc_feature    1048..1056
                     /note="NF-kappa B-related sites"
     GC_signal       1081..1086
                     /gene="TNF-beta"
     TATA_signal     1113..1118
                     /gene="TNF-beta"
     exon            1140..1281
                     /number=1
                     /gene="TNF-beta"
     prim_transcript 1140..3135
                     /gene="TNF-alpha"
     intron          1282..1633
                     /number=1
                     /gene="TNF-beta"
     repeat_region   1351..1390
                     /standard_name="dinucleotide repeat"
     repeat_region   1590..1629
                     /standard_name="dinucleotide repeat"
     exon            1634..1738
                     /number=2
                     /gene="TNF-beta"
     intron          1739..1820
                     /number=2
                     /gene="TNF-beta"
     misc_feature    1820..1828
                     /note="NF-kappa B-related site"
     exon            1821..1920
                     /number=3
                     /gene="TNF-beta"
     intron          1921..2141
                     /number=3
                     /gene="TNF-beta"
     exon            2142..3135
                     /number=4
                     /gene="TNF-beta"
     3'UTR           2555..3135
     misc_signal     2875..2882
                     /function="reduces mRNA stability"
                     /note="TTATTTAT element"
                     /note="found in 3'UTR of some cytokine genes"
     polyA_signal    3114..3119
                     /gene="TNF-beta"
     misc_feature    3629..3638
                     /note="NF-kappa B-related site"
     misc_feature    3772..3780
                     /note="NF-kappa B-related site"
     repeat_region   3965..4012
                     /standard_name="dinucleotide repeat"
     protein_bind    4018..4024
                     /bound_moiety="MHC class II Y box binding factor"
                     /standard_name="Y box"
     misc_feature    4062..4072
                     /note="NF-kappa B-related site"
     GC_signal       4222..4227
                     /gene="TNF-alpha"
     TATA_signal     4242..4247
                     /gene="TNF-alpha"
     5'UTR           4270..4431
     prim_transcript 4270..6858
                     /gene="TNF-alpha"
     exon            4432..4617
                     /number=1
                     /gene="TNF-alpha"
     intron          4618..5097
                     /number=1
                     /gene="TNF-alpha"
     exon            5098..5152
                     /number=2
                     /gene="TNF-alpha"
     intron          5153..5300
                     /number=2
                     /gene="TNF-alpha"
     exon            5301..5348
                     /number=3
                     /gene="TNF-alpha"
     intron          5349..5630
                     /number=3
                     /gene="TNF-alpha"
     exon            5631..6858
                     /number=4
                     /gene="TNF-alpha"
     3'UTR           6050..6858
     misc_signal     6532..6560
                     /function="reduces mRNA stability"
                     /note="TTATTTAT element"
                     /note="found in the 3' UTR of some cytokine genes"
     polyA_signal    6859..6865
                     /gene="TNF-alpha"
     misc_feature    7082..7091
                     /note="NF-kappa B-related site"
     CDS             join(4432..4617,5098..5152,5301..5348,5631..6049)
                     /gene="TNF-alpha"
                     /product="tumor necrosis factor"
                     /codon_start=1
                     /translation="MSTESMIRDVELAEEALPKKMGGLQNSRRCLCLSLFSFLLVAGA
                     TTLFCLLNFGVIGPNKEEKFPNGLPLISSMAQTLTLRSSSQNSSDKPVAHVVANHQAE
                     EQLEWLSQRANALLANGMDLKDNQLVVPADGLYLIYSQVLFKGQGCPDYVLLTHTVSR
                     FAISYQEKVSLLSAIKSPCPKDTPEGAELKPWYEPMYLGGVFQLEKGDLLSAEVNLPK
                     YLDITESGQVYFGVIAL"
     CDS             join(1643..1738,1821..1920,2142..2554)
                     /gene="TNF-beta"
                     /product="lymphotoxin"
                     /codon_start=1
                     /translation="MTPLGRLHLLRVLSTPPVFLLGLLLALPLGAQGLSGVRFSASRT
                     AHQPPQKHLTHGLLKPAAHLVGYPSKQNSLLWRANTDRAFLRHGFSLNNNSLLIPTSG
                     LYFVYSQVVFSGESCSPRAIPTPIYLAHEVQLFSSQYPFHVPLLSAQKSVYPGLQGPW
                     VRSMYQGAVFLLSKGDQLSTHTDGISHLHFSPSTVFFGAFAL"
BASE COUNT     1647 a   2009 c   1837 g   1612 t
ORIGIN      
        1 gagctctcct cagatgcggc ttctaaacaa gccctttaac tgtcttcacc tgtgtagaat
       61 tctgaagctc cctctataca gaactttgga agcccggggt atatatattt ggggttacat
      121 aggtctgatt tgcagggact tgcaggaacg ggctgagacc tccttgctat acatgagtgt
      181 cttgaggttc taagagaata cccccaaatc atcttctaga cctggaccat tctaggacag
      241 tggttctcga ccttcctaac tccatcaccc cttacttaat acggttcctc gtgttgtggt
      301 gaccccaacc atacaattat tttcattgct atttcataac tgtaattttg ctgttgctgt
      361 tatgaatcat aatgtaaata ttctctttaa atggaggttt gccgaagggt ctcgcccaca
      421 ggttgagaac tggtgctcca gagagtaagg ggatgcagct aagattgtca aacaccagga
      481 ggccccagat tctgggggag ggtgcactgt aatggtactt cttgccctga aactggccga
      541 tggccagaaa acacagatat actggaacag ctgtgacttg aacccccaaa gacaagagag
      601 gaaatggacc cgaaggggta gaggtcagtg gtggaaggca tgtcaagcgt acataacccc
      661 gggttcgaac ctgaaaaaac aaaggtgcca ctaaccacat gtggcttcag agccctccag
      721 acgtgaccag ggtcgatgga gagagaaacg ggcagaggag cttgtgagca gtgaagggtg
      781 cagaagtgtt ataaaccatc gctcttcgga gaaccaggct tccagtcaca gcacagctgt
      841 accccccctc attctccgcg aattagccgt ccgttcactg gaactcctca gcctgaccct
      901 ggtccctgct agtccctgca gcccacagtt ccccggaccc ggctcccttt cccagaacgc
      961 agtagtttga gcacctagcc tgcggttctc tcctaggccc cagcctttcc tgccttcgac
     1021 tgaaacagca gcatcttcta agccctgggg gcttccccag ccctagcccc gacctagaac
     1081 ccgcccgctg cctgccacac tgccgcttcc tctataaagg ggcccggagc accagcaccc
     1141 aggaccccgc acagcaggtg agcctctcct gccctgtctc cttgggctta ccccggtaac
     1201 aggcatccct caggatcctg cctcttctct tgagccacag tcttttctgt acaacctgcc
     1261 tggatccccc agccttaatg ggtctggtcc ttgtgccttg tggctttgat ttttggtctg
     1321 ttctcatagc gtctaactcg gccttgtcag tctctctctc tctctctctc tctctctctc
     1381 tctctctctc tgtcattgtc tcattctttg gtggagcttt cctcttccag tctggctttc
     1441 tctccttatc cccactcact tcagggttcc cctgcctgtc tgtccccttt tctgtctgtc
     1501 gccctgtctc tcagggtggc tgtctcagct gggaggtaag atctgtcttc cgctgtgtgc
     1561 cccgcctccc ctacacacat acacacatcc tctctctctc tctctctctc tctctctctc
     1621 tctctctctg caggttctcc ccatgacacc acttggacgt ctccacctcc tgagggtgct
     1681 tagcacccct cctgtcttcc tcctggggct gctgctggcc ctgcctctag gggcccaggt
     1741 gaggcagcaa gagagatggg ggtgctgggg cggcctggcc aactcagagt cctagaaccc
     1801 tctcaactct tctctcctag ggactctctg gtgttcgctt ctcagcttcc aggacagctc
     1861 accagccccc tcagaagcac ttgacccatg gcctcctgaa acctgctgct caccttgttg
     1921 gtaaacttct cctccagagg tccagccccg cttttcctcc tacttgccca ggggcccaag
     1981 cgatcttccc atctccccac accaactttt cttacccctc aaggcaggca ccctactccc
     2041 atcgccccac cagccatccc agttgtccag tatcttttcc ctgagggggg aagaagacct
     2101 ctgaccctga tctcacccca gtgccttgtg cctttcccca gggtacccca gcaaacagaa
     2161 ttcactgctc tggagagcaa acacggaccg cgcctttctc cgacatggct tctctttgaa
     2221 caacaactct ctcctgatcc ccaccagcgg cctctacttt gtctactccc aggtggtttt
     2281 ctctggagaa agctgctccc ccagggccat tcctactccc atctacctgg cccacgaggt
     2341 ccagctgttt tcctcccagt accccttcca tgtgcctctc ctcagcgcac agaaatctgt
     2401 gtatccgggg ctacaaggac cgtgggtacg ctcaatgtac cagggggctg tgttcctgct
     2461 cagtaagggg gaccagctgt ccacccacac agacggcatc tcccacttac acttcagccc
     2521 cagcaccgtg ttttttggag cttttgctct gtagaatcta gggaaactca ggaactggat
     2581 tccagcacct ctatcctgtt catcttgagg gtcacatccc cagtctccag ccttcccaac
     2641 caaaatagcc tggagctttc acgggagtct gaagcatttc aaggcaccac gtctctcccc
     2701 cagggccact ccagatgctc agggggagac tcaagcctgc ctagaagttc ctgcacagag
     2761 cagggtattt gtgggtctag gcggggcaga gacctggaca tgaaggaggg acagacatgg
     2821 ggggaggtgt ctggggacgg gggaggttga ctatttatga agagaagggt taagttattt
     2881 atttataggg aataaaaaga ggggagagat ggaaagccgt cgggtgacaa ctaggtccca
     2941 gacacaaagg cgtcagttac cacagaacag gactcatctg agagagggac ggtgacggtg
     3001 agagaagtag atgtaggtga ccaaggggtt ttagaagaaa gcccgaggct ctaaaagcca
     3061 accactgcct ggccacacat ccacagggag cccctgcacc atccgtgaat cccaataaac
     3121 ctcttttccc tgaaattctg tctgcttgtg tctgtcttgg attgggggag aatgttcctg
     3181 gtctctttaa ggagtggagc aggggacaga ggcctcagtt ggatcatggg agtcgacaga
     3241 gcaaggagac ataaaggagc aggcagctcc cagtaacatg atggattcgt gggagtgagg
     3301 caggctaagt gccggagaga cccaaaggat aaggctaggg agatcaatcc aaggttgggg
     3361 agagacggag gttctgggag aagtgactcc cccagagggt gggagagtgt ccaggactgg
     3421 gagggaaggg gagaggaatc cttggaagac atagagctat gggttgggag aaaccctgaa
     3481 agcagggttg tgggaaccac atgtctgaga tgtgctgcaa tcaagacgat atggcagaag
     3541 cttcgtggaa aactcacttg ggagcaggga ccgaaagcag cagtctaagc cccatgatca
     3601 gaatgaaagg agaaggcttg tggggtccgt gaattcccag ggctgagttc attccctctg
     3661 gggctgcccc attctcctcc agttacccca ccggccctcc cagagcccat acacactccc
     3721 agctctcaag ccactctgcc ttcagccact tcctctaaga actcagacaa gggggctttc
     3781 cctcctcaac gttgagtctc cccccttatg cagccagctg tcagaagcac tcccaatgct
     3841 aagttctccc ccatggaagt cctgtttaga aatcaaaggg gaacggacat aggcatggtg
     3901 tctctgcaga gaaacaatgg aagacagaaa ggaaatgggt ttcagttccc agggttctat
     3961 acatcacaca cacacacaca cacacacaca cacacacaca cacacacaca cacgttcctg
     4021 attggcccca gattgccaca gaatcctggt gaggacggag aggagattcc ttgatgcctg
     4081 ggtgtcccca actttccaaa cctctgcccc cgcattggag aagaaactga gagaggtgca
     4141 gggccactac cgcttcctcc acatgagatc atggttttct ccaccaagga agttttccgc
     4201 gggttgaatg agagcttttc cccgccctct tccccaaggg ctataaaggc agccggtaca
     4261 cagccagccg gcagaactca gcgaggacac caagggacca gccaggaggg agaacagcaa
     4321 ctccagaaca ccctggtact aactcccaga aaagcaagca accagccagg caggttccgt
     4381 ccctctcata cactggcccg aggcaacaca tctccctccg gaaaggacac catgagcacg
     4441 gaaagcatga tccgagatgt ggaactggca gaggaggcgc tccccaaaaa gatggggggc
     4501 ctccagaact ccaggcggtg tctgtgcctc agcctcttct cattcctgct cgtggcgggg
     4561 gccaccacgc tcttctgtct actgaacttc ggggtgatcg gtcccaacaa ggaggaggtg
     4621 agtgcctggg cagcgtttat tctcactcac aagcaaagta gggtgggagg gcaagaaagg
     4681 caggagggta agggacagag gtcggctggt gggcacagta atgtggagga gagttgggga
     4741 gggagacctt ggacccatgc agccagttgt ctaaacatcc ttgggtggat gcacggaggg
     4801 tgaatgaacg aacaagcgtg tgtacacgtg cagagatgtg cagagacgtg gccaggggaa
     4861 cagggaagaa gcaagagata aaaattcgag acggagatgg gagacgaggg agataaggag
     4921 ggagatggag gggaaacaac agatcaagca cagagggtac cacagaaaga tgctgggatt
     4981 ggacggataa atgaatgagg aggaaaagca ctgacttggg gggggggggg ctggcaaaga
     5041 tcggggaggc ttctttgtgg tgagtctgtc tactaaccta cttctttccc tacacagaag
     5101 ttcccaaatg ggctccctct catcagttcc atggcccaga ccctcacact cagtaagtgt
     5161 tcccaagcct ctctcttaat ttaggatggg gggcggggga cttaaagctt tggtttggga
     5221 ggaaagggaa taggggtcta actacacaga tgctttctgg gaaacctaag ggtctcatct
     5281 ccgcctttgt ccacttccag gatcatcttc tcaaaactcg agtgacaagc ccgtagccca
     5341 cgtcgtaggt aagtttcttc gaatgtgcct tggggggggt gtgacagggc aggattttgg
     5401 ggtcgtgcca ggacgagaga agacaagggc tctgagagac agcaggtgag agagtcagag
     5461 gggtgattca aaggcaaggc attaggaggc caccccggac ctcatagcca atgggacatg
     5521 gaaagacaag agggcacagg aaccggaagt gaagtgtggg tagaagttga ggttcaggat
     5581 gtggagtgtg aactaagagg gtgacactga ctcaattctc cctccctcag caaaccacca
     5641 agcagaggag cagctggagt ggctgagcca gcgtgccaac gccctcctgg ccaatggcat
     5701 ggatctcaaa gacaaccaac tggtggtacc agcagatggg ctgtacctta tctactccca
     5761 ggttctcttc aagggacaag gctgccccga ctatgtgctc ctcacccaca ccgtcagccg
     5821 atttgccatt tcataccagg agaaagtcag cctcctctcc gccatcaaga gcccttgccc
     5881 taaggacacc cctgagggag ctgagctcaa gccctggtat gagcccatgt acctgggagg
     5941 agtcttccag ctggagaagg gggacctgct cagcgctgag gtcaacctgc ccaagtactt
     6001 agacatcacg gagtccgggc aggtctactt tggagtcatt gctctgtgaa gcgactggcg
     6061 tgttcatccg ttctctaccc agcccctgtc cccgactctg acccccatta ctctgacccc
     6121 tttatcgtct actcctcaga gcccccaatc tgtgtccttc taacttagaa agggaattgt
     6181 ggctcagggt ccaactccgg gctcagaatt tccaacaact acgatgctca gaaacacacg
     6241 agatgctgaa gtagtggcct ggattgcggg ctgctcatga gccgccaccg gcaaggattc
     6301 caatgggctt tcggaactca ctggatcccc gaatgtcgat gcctgagtgg atcgggggag
     6361 tggagggggg agtggatcag gctgtctctg gctcacaagg ctgctgaaga tgtctcaggc
     6421 ctcccttcct tccttccttc cctccctcag agcttcccag actgttccct gaggcacagc
     6481 gcccagcctt ccttacggaa ccccctatat ttataattgc acctgtgact atttatttat
     6541 tatttattat ttatttattt gcttatgaat gtatttattt ggaaggccgg ggcgtcctgg
     6601 aggacccagc gttgggaagc tgtcttcagg ccaacatgtt ttctgtgaaa acggagctaa
     6661 actaccagct atccccatct ggtcccccac ccccgcctcg ttgcctcccc cttttctttt
     6721 gcttatgttt aaaacaagat atttatctaa cccagttgtc ttaataatgc tgatttggtg
     6781 accaggctgt cgctacatca ctgaacctct gctccccacg ggagccgtga ctgtaatcgc
     6841 cctacgggtc attgagagaa ataaagttcg cttggaaaag aaatgtgatt tctgaagtct
     6901 gcgtctgtcg tctctctgca gcctcctaaa gtctgtgggt ccagatctca gtttttatat
     6961 cccccaggga cattaagacc cctaagaccc tccaagaccc ctgtggaaca aaaggcagtc
     7021 cacaacccta cctctccccg gaaacaggag cctaagccta attacctttg cccctggtcc
     7081 tgggaatttc ccactctggg aattc
//