From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: S.pombe rad3+ gene
Date: 28 Dec 92 21:47:29 GMT
Distribution: bionet
Checksum: 27817 8

LOCUS       YSPRAD3      4217 bp ds-DNA             PLN       02-DEC-1992
DEFINITION  S.pombe rad3+ gene
ACCESSION   X63544
KEYWORDS    rad3+.
SOURCE      Schizosaccharomyces pombe DNA.
  ORGANISM  Schizosaccharomyces pombe
            Eukaryota; Fungi; Eumycota; Hemiascomycetes; Endomycetales;
            Saccharomycetaceae.
REFERENCE   1  (bases 1 to 4217)
  AUTHORS   Seaton,B.L., Yucel,J., Sunnerhagen,P. and Subramani,S.
  TITLE     Isolation and characterization of the Schizosaccharomyces pombe
            rad3 gene, involved in the DNA damage and DNA synthesis checkpoints
  JOURNAL   Gene 119, 83-89 (1992)
  STANDARD  full automatic
COMMENT     *source: clone_library=pFL20 genomic; *source: clone=pSub1.41;
            *source: chromosome=II;
               From EMBL    entry SPRAD3;  dated 13-OCT-1992.
FEATURES             Location/Qualifiers
     TATA_signal     106..111
     TATA_signal     212..217
     polyA_signal    3932..3937
     CDS             331..3543
                     /gene="rad3+"
                     /codon_start=1
                     /translation="MGKKPNIASLFAQLMNISEGDFLIRTQAYTLPFLVLTKNKALIV
                     RIAELSQSDVATLCLTNMHKILASLLTTDHPNLEESVMLLLSLATSDFEKVDLTSLLR
                     SDPISITVELLQLYQNDVPHEKIENALRKVAMIVSQVVNDEDLSNKELLYDFFNNHIL
                     GILAEFSNILNDLKGKTSINEKIKTIVGIEKMLSLCGGAVKLGLPQILSNLQSAFQNE
                     HLRFYAIKAWFSLILATKEPEYSSIAGLSLVILPPLFPYLEPQEAELVIQIFDFISSD
                     THKCLQGLKWAIPTSLDSACFSLKAKEIFCSLQNEDFYSELQSIIKCLTNENEPVCYL
                     GLQKLELFFQAKVDELHDTLNLDISNEVLDQLLRCLLDCCVKYASTNMQISYLAAKNL
                     GELGAIDPSRAKAQHIIKETVVLDNFENGEESLKFILDFMQSQLIPAFLVTTDTKAQG
                     FLAYALQEFLKLGGFKSAVINKKKGLTVVTEHWMSLPDLSKRVLIPFLTSKYHLTPIP
                     KIDIRYPIYKENVTIHTWMQLFSLKLMEYAHSQNAEKIFGICSKVVKDQEVNIPCFLL
                     PFLVLNVILTESELEVNKVIEEFQLVINQPGPDGLNSVGQQRYTSFVDVFFKIVDYLN
                     KWLRMRKKRNWDRRSAIARKENRYMSVEDATSRESSISKVESFLSRFPSKTLGIVSLN
                     CGFHARALFYWEQHIRNATAPYAALESDYRVLQEIYAGIDDPDEIEAVSLNFHDYSFD
                     QQLLLHENSGTWDSALSCYEIIIQKDPENKKAKIGLLNSMLQSGHYESLVLSLDSFII
                     NDNHEYSKMLNLGIEASWRSLSIDSLKKCLSKSNLESFEAKLGSIFYQYLRKDSFAEL
                     TERLQPLYVDAATAIANTGAHSAYDCYDILSKLHAINDFSRIAETDGIVSDNLDIVLR
                     RRLSQVAPYGKFKHQILSTHLVGYEKFENTKKTAEIYLEIARISRKNGQFQRAFNAIL
                     KAMDLDKPLATIEHAQWWWHQGQHRKAISELNFSLNNNMFDLVDEHEERPKNRKETLG
                     NPLKGKVFLKLTKWLGKAGQLGLKDLETYYHKAVEIYLRM"
BASE COUNT     1340 a    732 c    791 g   1354 t
ORIGIN      
        1 cattttcatt aagggaccta ataatcttga tatagttgaa tttcacaagg aaagtaaagc
       61 cttgattttt aatacgttga aaatattggc ggtggaaaat acagctattt tagaacgtca
      121 tcttcctgga tctccttatc tagagtggta gaagaagaag aattacattt tgtactattg
      181 gaagttatat cttctgtgat aaacagcgga atattttatc aaggcattgg tctcagcgct
      241 ctgcaacaaa ttgcctcgac gcgtcatata tccgtttggc aattactttc tccatattgg
      301 ccaacagtgt ccgttgcgat tgtccaaggt atgggtaaaa aaccgaacat agccagttta
      361 tttgctcagc ttatgaatat ttccgagggc gattttctta ttcgaacaca ggcgtacact
      421 ttaccattcc ttgtacttac taaaaacaaa gcgttaatag tacgtatagc tgaactttca
      481 caaagtgatg ttgctacttt gtgccttacc aatatgcata aaatccttgc ttcgctactt
      541 actacggatc atcctaattt ggaagagagt gtgatgcttc ttctttcact ggccacttct
      601 gattttgaaa aagttgattt aacgtctttg ttacgctctg atcctatttc tattactgtg
      661 gagttgttac agctttatca gaatgatgtt cctcatgaaa aaattgaaaa tgctttaaga
      721 aaggtagcaa tgattgtctc tcaagtggtt aatgacgaag acttgagcaa taaggaatta
      781 ctttatgatt tttttaataa tcacattttg ggtatcttag cagaattttc taatatcctt
      841 aacgacctga aaggaaagac ttcaattaat gaaaagatta agacaattgt cggcattgaa
      901 aaaatgttat ctttatgtgg aggtgcagtc aaacttggat taccacagat actttctaat
      961 ttacaaagtg cttttcaaaa tgagcactta aggttttatg caatcaaagc ttggttcagt
     1021 ttgatattag caaccaagga gcccgagtat agttcaattg ctggtttaag tcttgtaatt
     1081 ttacctcctt tattccctta tttagaacca caagaagcag agctagtaat tcaaatattt
     1141 gattttattt cttctgacac acacaagtgc ctacaaggat taaagtgggc tatccccacc
     1201 agtctggatt cagcgtgctt tagccttaag gctaaagaaa tattctgttc gcttcaaaat
     1261 gaagattttt actctgagct tcaaagtata attaagtgtt taactaacga aaatgagcca
     1321 gtttgttatt taggtttaca aaaattagaa cttttttttc aagccaaggt ggacgagtta
     1381 catgacacac taaatttgga catatccaac gaagttctgg accaattact aagatgcctt
     1441 ttagattgtt gtgtaaaata tgcttcaaca aatatgcaaa tatcatatct tgctgcaaaa
     1501 aatcttggtg aattgggtgc gatagatccc agccgcgcca aggctcaaca tattattaaa
     1561 gaaacagttg ttcttgataa ctttgaaaac ggagaagaaa gtttgaagtt tattctagat
     1621 tttatgcaat cgcagttaat tccagctttc cttgttacta ctgatactaa agcacaaggt
     1681 tttcttgcct atgctctgca agagtttcta aagcttggtg gattcaagtc cgcagtgatt
     1741 aataaaaaaa agggactaac tgtggtaaca gaacattgga tgtctttgcc tgatttatcc
     1801 aaacgtgtgc ttataccatt tttaacttcc aagtatcatt taacaccaat ccccaaaatt
     1861 gacattcggt accctattta taaagaaaat gttactattc atacttggat gcagttgttt
     1921 tctcttaaat tgatggagta cgcccattcg caaaacgctg aaaaaatatt tggtatttgt
     1981 tcgaaagtag tgaaagacca agaggttaac attccctgtt ttcttcttcc ctttcttgtt
     2041 ttaaatgtta ttttaaccga gtcagaactg gaagttaata aagtcattga agaattccag
     2101 cttgttatta atcaaccggg acctgatgga ttaaattccg tggggcaaca aagatacacc
     2161 tcatttgtag atgtattttt taagattgtg gattacctta acaaatggct tcgcatgcga
     2221 aagaagagga attgggatag acgttctgcc attgcaagga aagagaaccg ttatatgtcg
     2281 gtggaagatg ctacctctcg agaatcatcg atctcaaaag ttgagtcatt tctttctcga
     2341 tttccttcaa aaacattagg tattgtctct ttaaattgtg gatttcatgc tcgtgcattg
     2401 ttttattggg agcaacacat acgtaatgct acagctccat atgcagcttt agagtccgat
     2461 tatagagttt tgcaggaaat atatgctgga attgatgatc cagatgaaat cgaagcagtg
     2521 tctttaaatt tccatgatta ctcgtttgat caacaactcc ttttacatga aaattcagga
     2581 acatgggact cggctttgag ttgttacgaa attattattc aaaaggatcc tgaaaataaa
     2641 aaggcgaaaa tcggtttgct taacagcatg ctgcaatcgg ggcattatga atctcttgtt
     2701 ttgagtttag attcttttat aatcaatgac aaccacgagt attcgaagat gttaaatttg
     2761 ggtattgaag cttcatggcg ttcgctatct attgattcgt taaaaaagtg tctttcaaaa
     2821 agcaacttgg aatctttcga agctaaattg ggtagcatat tttaccaata cctacggaag
     2881 gattcttttg ctgaattgac ggagcggctg caacccttgt acgttgatgc tgctacagca
     2941 attgcaaaca caggcgccca ttcagcctat gattgttatg atattttatc taagctgcac
     3001 gcaattaatg actttagtag gattgctgaa actgacggaa ttgtttccga caatcttgat
     3061 attgttcttc gccgtcggct tagccaagta gctccgtacg gtaaattcaa gcaccaaatc
     3121 ctgtccactc acttagttgg ctatgaaaaa tttgaaaaca cgaagaaaac tgctgaaata
     3181 tatctcgaga ttgcaagaat atctcgaaaa aatggtcaat ttcaaagagc cttcaatgcc
     3241 atcctcaaag caatggattt agataaaccg ctagcaacaa tagagcacgc acaatggtgg
     3301 tggcatcaag ggcaacatcg taaagctatt tctgaattga atttttcgct taataacaac
     3361 atgtttgatt tggttgatga gcatgaagaa agacctaaaa atcgtaaaga aactttagga
     3421 aatccactta aaggaaaagt gttcttgaaa cttacaaaat ggctcggaaa agctggccaa
     3481 ctgggattga aggatttgga gacgtattat cataaagcgg tagagattta cctcagaatg
     3541 tgagaatacg cattattatc ttggccatca tcgagtttta atgtatgaag aagaacaaaa
     3601 gctcccagtt aatgaacaga gcgaacgatt tttaagtggt gagttagtaa ctcgcataat
     3661 taacgaattt ggtcgatctt tgtactatgg tacaaatcat atatatgaaa gtatgccaaa
     3721 attgctcaca ctgtggcttg attttggggc cgaagaactt cgcttatcta aagatgacgg
     3781 cgaaaagtac tttcgtgaac acattatctc ttcgagaaaa aaatctttgg aacttatgaa
     3841 ttcgaatgtt tgtcgccttt ctatgaaaat tcctcaatac ttttttctgg ttgcattatc
     3901 ccaaatgata tccagagtat gccatccaaa taataaagtt tataaaattt tggaacatat
     3961 aattgcaaac gttgtagcat cttatcctgg ggagacgcta tggcaattaa tggcaacaat
     4021 aaaatcgact tctcaaaagc gctcgcttcg tggaaaaagc attttaaatg ttttacattc
     4081 taggaagctt tctatgtctt ccaaagttga tataaaagca ctcagtcaat ctgcaattct
     4141 cattactgaa aagttaatca atttgtgcaa tacaaggatt aacagtaaat ctgtaaaaat
     4201 gagcttaaag gatccac
//