From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Psophocarpus tetragonolobus DNA for WCI-3.
Date: 6 Jan 93 07:01:57 GMT
Distribution: bionet
Checksum: 46322 5

LOCUS       PSTWCI3A     1872 bp ds-DNA             PLN       06-JAN-1993
DEFINITION  Psophocarpus tetragonolobus DNA for WCI-3.
ACCESSION   D13975
KEYWORDS    WCI-3.
SOURCE      Psophocarpus tetragonolobus (cultivar ishigaki4, strain (L.)DC. )
            Mature Leaf DNA, clone pWBG-5.
  ORGANISM  Psophocarpus tetragonolobus
            Eukaryota; Plantae; Embryobionta; Magnoliophyta; Magnoliopsida;
            Rosidae; Fabales; Fabaceae.
REFERENCE   1  (bases 1 to 1872)
  AUTHORS   Habu,Y., Peyachokunagul,S., Umemoto,K., Sakata,Y. and Ohno,T.
  TITLE     Structure and regulated expression of Kunitz chymotrypsin inhibitor
            genes in winged bean (Psophocarpus tetragonolobus (L.) DC.)
  JOURNAL   J. Biochem. 111, 249-258 (1992)
  STANDARD  full automatic
REFERENCE   2  (bases 1 to 1872)
  AUTHORS   Habu,Y., Sakata,Y., Fukasawa,K. and Ohno,T.
  TITLE     Ubiquitous nuclear proteins bind to multiple sites in 5' upstream
            region of major Kunitz chymotrypsin inhibitor gene in winged bean
            (Psophocarpus tetragonolobus (L.) DC.)
  JOURNAL   Unpublished (2048)
  STANDARD  full automatic
COMMENT     Submitted (24-DEC-1992) to DDBJ by: Yoshiki Habu Department of
            Applied Bioscience Faculty of Agriculture Hokkaido University Nishi
            9, Kita 9 Kita-ku, Sapporo Hokkaido 060 Japan Phone:  011-716-2111
            x3887 Email:  yoshiki_habu%al%hines@xmail-gw.sys.hokudai.ac.jp Fax:
               011-746-0208.
FEATURES             Location/Qualifiers
     TATA_signal     1041..1047
     5'UTR           1076..1115
     mRNA            1076..1850
     sig_peptide     1116..1187
                     /codon_start=1
     mat_peptide     1188..1736
                     /product="WCI-3"
                     /codon_start=1
     3'UTR           1740..1850
     polyA_signal    1797..1802
     polyA_signal    1817..1822
     CDS             1116..1739
                     /standard_name="winged bean major Kunitz chymotrypsin
                     inhibitor gene A"
                     /product="WCI-3"
                     /codon_start=1
                     /translation="MKSTTFLALFLLSAIISHLPSSTADDDLVDAEGNLVENGGTYYL
                     LPHIWAHGGGIETAKTGNEPCPLTVVRSPNEVSKGEPIRISSQFLSLFIPRGSLVALG
                     FANPPSCAASPWWTVVDSPQGPAVKLSQQKLPEKDILVFKFEKVSHSNIHVYKLLYCQ
                     HDEEDVKCDQYIGIHRDRNGNRRLVVTEENPLELVLLKAKSETASSH"
BASE COUNT      571 a    308 c    292 g    701 t
ORIGIN      
        1 tctctccacc tttctcaata agaacatttt tacaattatt taaaatataa aaaattatta
       61 ttaaatgtca aaagattcaa ttttcgtatc tcctttcttt cttttctaat taaaaaatat
      121 gagtcacgct ttattttttt tatttattat gtaagtcata aatgataaag cattttttat
      181 taatggaaca aaaaatgcaa gattttcgtc tatattatat gcccacctac attttattat
      241 ttctatctat tatttttttt ctataattct atctcatatt aattattata ttaataaata
      301 ttttctctct tgtgatgcct aataacatgt ttaggtgtcc taaaattttt taataaatat
      361 atcaagttca cgtacagtat atttaaacta cctagatatt tattgaaatt tatttttggc
      421 aatctcttta ttttattttt ggtgaagtct ttctttcttc tggattttcc attgagtttt
      481 ggtacgtttt atattttgtt catctccatt atgagtggag tcatttggtt tgtgtcacga
      541 ctatatttat cagtacatgc attggctaga ttgacggtat attatgtcat gacttgagtc
      601 ttgaggcttg ttggttgtgt attacgtttc atgggtacac gtatagataa gatttgaagg
      661 ccaattttgt tgtgaacgat aaaagggtat ctctatatgc ataaaatagc tttaataact
      721 gttttcctag aaaatagcat tgtagacttt ttgttttatg ttagtgttta aacatgttta
      781 ttttatttta gttctatgag ctgctgtaat ttttcatgta atgcaaagga gtcttaaaca
      841 ttagaggata aactggtata attttctttt ttctataaat tatattttaa tcacttataa
      901 gctagtttca tgtctaaatt ataatgcatt aataagaaat taaaaacaaa gttttgtcta
      961 aaaatttata ccagactatt tacaaattcg tttaattaat aatgttatcg aaaacacagt
     1021 ttatagttaa cgagcacttc tataaatagt ctacattcgg cgaaacctat tctctcaaat
     1081 gagtagaaag gaaatcgttc cacaagcata aaaaaatgaa gagtactaca tttcttgccc
     1141 tctttctact ctctgccatc atctcacacc taccatcatc cactgctgat gatgatttgg
     1201 tcgatgctga aggtaactta gttgaaaatg gtggcacata ctatctgttg ccacatatat
     1261 gggcacacgg gggaggaata gaaacagcaa aaacaggaaa cgaaccatgc cctctaacag
     1321 tggtacgatc tcccaatgag gtctctaaag gggaaccaat aaggatctca tcccaattcc
     1381 tttcattgtt catccccaga ggctctctag tggctcttgg attcgctaac cctccatctt
     1441 gtgcagcttc tccgtggtgg actgttgttg actctccaca aggacccgct gttaaactta
     1501 gtcagcaaaa acttccggaa aaggatattc tagtgtttaa attcgagaaa gtttcccatt
     1561 ctaacattca cgtgtacaag cttttgtact gtcaacatga cgaagaggat gtgaagtgtg
     1621 atcagtatat cgggattcat agggatcgca atggaaacag acgtttggtg gtgactgagg
     1681 aaaacccatt agagcttgtg cttctgaaag ctaagtcaga aactgcatca agccattaag
     1741 cacttctcat ccttaaaagt catctgtgct tatgactaaa tgaatgcttt gcttgtaata
     1801 aattgtcaca ccactcaata aaattatcta agtttttgct tttctttttt atttttgtgc
     1861 ttttaagaat tc
//