From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Arabidopsis thaliana alpha-4 tubulin (TUA4) gene, complete cds.
Date: 25 Jul 92 10:02:28 GMT
Distribution: bionet
Checksum: 32000     5

LOCUS       ATHTUBA4A    2233 bp ds-DNA             PLN       25-JUL-1992
DEFINITION  Arabidopsis thaliana alpha-4 tubulin (TUA4) gene, complete cds.
ACCESSION   M84697
KEYWORDS    alpha-4 tubulin.
SOURCE      Arabidopsis thaliana (strain Columbia) (library: cosmid pOCA18;
            Olszewski) DNA.
  ORGANISM  Arabidopsis thaliana
            Eukaryota; Plantae; Embryobionta; Magnoliophyta; Magnoliopsida;
            Dilleniidae; Capparales; Brassicaceae.
REFERENCE   1  (sites)
  AUTHORS   Kopczak,S.D., Haas,N.A., Hussey,P.J., Silflow,C.D. and Snustad,D.P.
  TITLE     The small genome of Arabidopsis contains at least six expressed
            alpha-tubulin genes
  JOURNAL   Plant Cell 4, 539-547 (1992)
  STANDARD  full staff_review
REFERENCE   2  (sites)
  AUTHORS   Snustad,D.P., Haas,N.A., Kopczak,S.D. and Silflow,C.D.
  TITLE     The small genome of Arabidopsis contains at least nine expressed
            beta-tubulin genes
  JOURNAL   Plant Cell 4, 549-556 (1992)
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     CDS             join(123..215,625..1230,1314..1967)
                     /product="alpha-4 tubulin"
                     /gene="TUA4"
                     /codon_start=1
                     /translation="MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMPSDKTVGG
                     GDDAFNTFFSETGAGKHVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLISGKEDAANN
                     FARGHYTIGKEIVDLCLDRIRKLADNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVD
                     YGKKSKLGFTVYPSPQVSTSVVEPYNSVLSTHSLLEHTDVSILLDNEAIYDICRRSLS
                     IERPTYTNLNRLVSQVISSLTASLRFDGALNVDVTEFQTNLVPYPRIHFMLSSYAPVI
                     SAEKAFHEQLSVAEITNSAFEPASMMAKCDPRHGKYMACCLMYRGDVVPKDVNAAVGT
                     IKTKRTIQFVDWCPTGFKCGINYQPPTVVPGGDLAKVQRAVCMISNSTSVAEVFSRID
                     HKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKDYEEVGAEGGDDEDDEGEEY
                     "
     intron          216..624
                     /number=1
                     /gene="TUA4"
     exon            625..1230
                     /number=2
                     /gene="TUA4"
     intron          1231..1313
                     /number=2
                     /gene="TUA4"
BASE COUNT      550 a    467 c    476 g    740 t
ORIGIN
        1 ttactaaaaa cctagaaata gaaaaatcag atctagattc agtttttttc atcaaaaaag
       61 agcgtcttca taaacgccct tcgtcttctt ccctggaaat ctagtttctt tcttccacga
      121 aaatgagaga gtgcatttcg atccacattg gtcaggctgg tatccaggtc ggaaatgctt
      181 gctgggagct gtactgtctt gaacatggca ttcaggttcg tatctagatc tgtgcttctt
      241 ctatattttt cgtaatttta taattcagat ctgagataat ttggccatgc attgaccttg
      301 ttctccggat tctgatgaat tcgtatatat ttagtacgta attgtttaga tctgtgtttg
      361 attccgatgt cttacaattt ttttatgtgg tttatgtaag atccgtttct cattttagaa
      421 atatttttga catacctgtt tagatctgag tttgacatag atttcttgtt ggttgattca
      481 agatctgttc gttgaacaaa aaaaatattc taggtttgtt tagaagatgc aaatcgatct
      541 atttcccata tacttaagct tctatatgct tagtgcatac tgtatgagtt tgtattgata
      601 tgttttggat ctggaatgtt acagcctgat ggccagatgc cgagtgacaa gactgttggt
      661 ggaggtgacg atgctttcaa caccttcttc agtgaaaccg gtgcaggtaa gcacgtccca
      721 cgtgctgtct ttgttgatct tgagccaact gtgatcgacg aggtcaggac tggtacttac
      781 cgtcagcttt tccaccctga acaactcatc agcggtaaag aagatgcagc taacaatttc
      841 gcccgtggtc attacaccat tgggaaagag attgttgacc tgtgcttaga ccgtatcaga
      901 aagcttgctg ataactgtac tggtctccaa ggattcctcg tcttcaacgc tgttggtgga
      961 gggactggat ctggtcttgg atctctcctc cttgagagac tttctgttga ctacgggaaa
     1021 aagtccaagt tgggtttcac agtttaccca tctccacagg tctctacctc cgttgttgag
     1081 ccttacaaca gtgtcctctc cactcactct ctcttggaac acactgatgt ctccatcctc
     1141 ctcgacaatg aagctatcta tgacatctgc agacgctccc taagcattga gagaccaacc
     1201 tacaccaacc tcaaccgcct cgtctctcag gtattgtagc cagcctcaac cagtcttttt
     1261 gctgttacat tttcttgggc tcatctaatg ttattttcct attttgtttt caggttattt
     1321 cttccttgac tgcttctctg aggtttgatg gtgccttaaa tgttgatgtc actgagttcc
     1381 aaaccaactt ggtcccatac ccaagaatcc acttcatgct ttcctcctat gccccagtca
     1441 tctccgcaga gaaagccttc catgagcaac tctcagttgc tgagatcaca aacagtgctt
     1501 ttgagccagc ttccatgatg gctaagtgtg acccacgtca cggaaagtac atggcttgct
     1561 gtttgatgta ccgtggtgat gttgtcccca aggatgtaaa cgcagctgtt ggcaccatca
     1621 agaccaagcg cactattcag tttgttgact ggtgtcctac tggattcaag tgtggtatca
     1681 actaccagcc accaacagtt gttccaggag gtgatcttgc taaagtccag agagctgttt
     1741 gcatgatctc aaactcgacc agtgttgctg aggtgttctc ccgtattgat cacaagtttg
     1801 atcttatgta cgccaaacgt gctttcgttc actggtatgt gggtgagggt atggaagaag
     1861 gagaattctc agaggcacgt gaggatcttg cagcattgga gaaggattat gaagaggtcg
     1921 gtgctgaagg tggtgacgat gaagatgatg aaggagagga atactaagaa gaatgtttct
     1981 aaaaactttg gatttgtgtg ggtttctcta taatctcgtc ttgtgagaat gggctcaaaa
     2041 ctcttgggag tctttaatcg tgtgtgtttt aaaacctact tctctatctt ttcgtagcca
     2101 tgttatctct ctattatcta tttcctttgt gtgttaaatc gtttctgcct ttctggtaat
     2161 atcttatcac tactatcaaa actacaaggg ttatttgaag gcatggaaaa aattcatgag
     2221 atgttatata ttt
//