From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Bacillus stearothermophilus DNA restriction endonuclease (VIR)
Date: 29 Dec 92 19:49:10 GMT
Distribution: bionet
Checksum: 09577 3

LOCUS       BACBSTVIR     871 bp ds-DNA             BCT       09-DEC-1992
DEFINITION  Bacillus stearothermophilus DNA restriction endonuclease (VIR)
            gene, complete cds.
ACCESSION   L07643
KEYWORDS    endonuclease; restriction-modification system.
SOURCE      Bacillus stearothermophilus (strain V) DNA.
  ORGANISM  Bacillus stearothermophilus
            Prokaryotae; Firmicutes; Endospore-forming rods and cocci;
            Bacillaceae.
REFERENCE   1  (bases 1 to 871)
  AUTHORS   Vasquez,C., Saavedra,C. and Gonzalez,E.
  TITLE     Cloning the BstVI restriction-modification system in Escherichia
            coli
  JOURNAL   Gene 102, 83-85 (1991)
  STANDARD  full automatic
REFERENCE   2  (bases 1 to 871)
  AUTHORS   Gonzalez,E., Saavedra,C. and Vasquez,C.
  TITLE     Sequence of the restriction-modification system from Bacillus
            stearothermophilus V
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     -35_signal      13..18
     -10_signal      33..39
     RBS             111..114
                     /note="putative"
     CDS             130..804
                     /function="cleaves the sequence 5'-CTCGAG-3' between C and
                     T"
                     /standard_name="BstVI endonuclease"
                     /gene="VIR"
                     /evidence="EXPERIMENTAL"
                     /citation=[1]
                     /product="DNA restriction endonuclease"
                     /codon_start=1
                     /translation="MNKQASGKDTGNRGAVTGGKQMDGFADMLTNLLSKVGISNHEVF
                     TSEKPHLPGYFRATKKWDFLVVKTNDRGEKYLLAAVELKSHVGPSFGNNLNNRVEESL
                     GSATDIWIAFREKAFKNSRAPWLGYLMLLEDCPQSTKPVKVDEPHFEVFPEFKNASYA
                     KRYELFCRKIVLERKYTSSCLLMSDKQGGLQGIYKEPANDLKIYPFLYSLLTHVATEA
                     ALISSS"
BASE COUNT      295 a    143 c    194 g    239 t
ORIGIN      
        1 gagattatta aagatgcatt ggaataggag gatattaaag gatgaatatc catataccgt
       61 ctgaactagg agataaagaa gcttaccggg gaagctgtta agtttttttg ggagactcgg
      121 gaaaagcaga tgaataaaca agctagtgga aaagataccg gtaatcgagg ggcagttacg
      181 ggtggaaagc aaatggatgg atttgcagat atgctaacca atttgttgag taaggtaggt
      241 atatctaatc atgaggtttt cacttcagaa aaaccacatt tgccaggata cttccgagca
      301 acaaaaaagt gggattttct agttgttaag acaaatgatc ggggcgaaaa atatcttctc
      361 gcagccgtag aattaaaatc ccatgtaggt ccttcatttg gtaataatct taataatcgc
      421 gttgaggagt ccttaggaag tgccactgat atttggattg catttcgaga aaaggccttt
      481 aaaaatagtc gtgcgccgtg gttgggttac ctaatgttac ttgaagattg tccccaatcg
      541 acaaagccag tcaaggtaga cgaaccacat ttcgaagtgt ttcctgaatt taaaaatgct
      601 tcatatgcga aacgatatga actgttttgt agaaaaattg ttctagaaag aaagtacaca
      661 tcatcctgcc tactaatgtc agataaacaa gggggactcc aaggtattta caaagagcca
      721 gcaaatgatt tgaaaatata ccctttctta tactcccttc ttacacatgt ggccacggag
      781 gcagcattaa tttcctccag ctaatcgaag aaaaagagga aacgtttgtt aacaagtgta
      841 atgattaaaa gttatttcgc taacggaatt c
//