From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Escherichia coli short chain alcohol dehydrogenase homolog (envM)
Date: 21 Dec 92 20:18:02 GMT
Distribution: bionet
Checksum: 58128 4

LOCUS       ECOENVM      1366 bp ds-DNA             BCT       18-NOV-1992
DEFINITION  Escherichia coli short chain alcohol dehydrogenase homolog (envM)
            gene, complete cds.
ACCESSION   M97219
KEYWORDS    envM gene; short chain alcohol dehydrogenase.
SOURCE      Escherichia coli DNA.
  ORGANISM  Escherichia coli
            Prokaryotae; Gram-negative facultatively anaerobic rods;
            Enterobacteriaceae.
REFERENCE   1  (bases 1 to 1366)
  AUTHORS   Bergler,H., Hogenauer,G. and Turnowsky,F.
  TITLE     Sequences of the envM gene and two mutated alleles in Escherichia
            coli
  JOURNAL   J. Gen. Microbiol. 138, 2093-2100 (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     misc_feature    144..152
                     /note="partial sequence of boxC"
     misc_feature    199..241
                     /note="boxC"
     misc_feature    254..342
                     /note="partial sequence of boxC"
     -10_signal      311..316
                     /gene="envM"
                     /note="putative"
     allele          680
                     /note="change in base causes diazaborine resistance (Gly
                     -> Ser)"
     allele          1125
                     /note="change in base produces ts-phenotype (Ser -> Phe)"
     CDS             404..1192
                     /note="envM"
                     /gene="envM"
                     /codon_start=1
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
BASE COUNT      307 a    368 c    355 g    336 t
ORIGIN      
        1 ctgcaggaac tgaaccgccg gtcaccctct ccctgaaaga gcgagggggc agaccgagcc
       61 gaatagctgt tgtggtgaaa acatggagac ggtgctggag aatattcggc aaggtctgaa
      121 ccgtcccagc catcgccatg aaagggttag gggctgtatg agcctgtttg ttgctggggt
      181 aacaatattt gcacaatacg gtcccctcgc ccctctgggg agagggttag ggtgagggga
      241 aaagcgcccc ccctgccgca gcctgctccg gtcggacctg gcaactatag ctactcacag
      301 ccaggttgat tataataacc gtttatctgt tcgtactgtt tactaaaacg acgaatcgcc
      361 tgattttcag gcacaacaag catcaacaat aaggattaaa gctatgggtt ttctttccgg
      421 taagcgcatt ctggtaaccg gtgttgccag caaactatcc atcgcctacg gtatcgctca
      481 ggcgatgcac cgcgaaggag ctgaactggc attcacctac cagaacgaca aactgaaagg
      541 ccgcgtagaa gaatttgccg ctcaattggg ttctgacatc gttctgcagt gcgatgttgc
      601 agaagatgcc agcatcgaca ccatgttcgc tgaactgggg aaagtttggc cgaaatttga
      661 cggtttcgta cactctattg gttttgcacc tggcgatcag ctggatggtg actatgttaa
      721 cgccgttacc cgtgaaggct tcaaaattgc ccacgacatc agctcctaca gcttcgttgc
      781 aatggcaaaa gcttgccgct ccatgctgaa tccgggttct gccctgctga ccctttccta
      841 ccttggcgct gagcgcgcta tcccgaacta caacgttatg ggtctggcaa aagcgtctct
      901 ggaagcgaac gtgcgctata tggcgaacgc gatgggtccg gaaggtgtgc gtgttaacgc
      961 catctctgct ggtccgatcc gtactctggc ggcctccggt atcaaagact tccgcaaaat
     1021 gctggctcat tgcgaagccg ttaccccgat tcgccgtacc gttactattg aagatgtggg
     1081 taactctgcg gcattcctgt gctccgatct ctctgccggt atctccggtg aagtggtcca
     1141 cgttgacggc ggtttcagca ttgctgcaat gaacgaactc gaactgaaat aatcgttctg
     1201 ttggtaaaga ctggcgttct gccgcccgtt atctctgtta tacctttctg atatttgtta
     1261 tcgccgatcc gtctttctcc ccttccgcct tgcgtcagga taacgatttc ctttacgacc
     1321 aaggagcgcc catggaacaa cgccacatca ccggcaaaag ccactg
//