From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Mouse pyruvate dehydrogenase (pdha-1) mRNA, complete cds.
Date: 30 Jul 92 10:02:09 GMT
Distribution: bionet
Checksum: 40636     4

LOCUS       MUSPDHA1A    1446 bp ss-mRNA            ROD       30-JUL-1992
DEFINITION  Mouse pyruvate dehydrogenase (pdha-1) mRNA, complete cds.
ACCESSION   M76727
KEYWORDS    lactic acidosis; mitochondrial matrix; pyruvate dehydrogenase.
SOURCE      Mus musculus (strain B6/CBAFIJ) female adult liver cDNA to mRNA.
  ORGANISM  Mus musculus
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Rodentia; Myomorpha; Muridae; Murinae.
REFERENCE   1  (bases 1 to 1446)
  AUTHORS   Fitzgerald,J., Hutchison,W.M. and Dahl,H.-H.M.
  TITLE     Isolation and characterization of the mouse PDH E1a genes
  JOURNAL   Biochim. Biophys. Acta 1131, 83-90 (1992)
  STANDARD  full staff_entry
FEATURES             Location/Qualifiers
     mRNA            1..1446
                     /gene="pdha-1"
     CDS             112..1284
                     /EC_number="1.2.4.1"
                     /product="pyruvate dehydrogenase"
                     /gene="pdha-1"
                     /codon_start=1
                     /translation="MRKMLAAVSRVLAGSAQKPASRVLVASRNFANDATFEIKKCDLH
                     RLEEGPPVTTVLTREDGLKYYRMMQTVRRMELKADQLYKQKIIRGFCHLCDGQEACCV
                     GLEAGINPTDHLITAYRAHGFTFTRGLPVRAILAELTGRRGGCAKGKGGSMHMYAKNF
                     YGGNGIVGAQVPLGAGIALACKYNGKDEVCLTLYGDGAANQGQIFEAYNMAALWKLPC
                     IFICENNRYGMGTSVERAAASTDYYKRGDFIPGLRVDGMDILCVREATKFAAAYCRSG
                     KGPILMELQTYRYHGHSMSDPGVSYRTREEIQEVRSKSDPIMLLKDRMVNSNLASVEE
                     LKEIDVEVRKEIEDAAQFATADPEPPLEELGYHIYSSDPPFEVRGANQWIKFKSVS"
     misc_feature    112..198
                     /note="mitochondrial import sequence"
                     /gene="pdha-1"
     polyA_signal    1393..1399
                     /gene="pdha-1"
BASE COUNT      395 a    306 c    419 g    326 t
ORIGIN
        1 gtgggaattt caggccaaaa ggggcagaga tgtaactatg tgtagaattg ggatcaactg
       61 tcaggaggag tgcctgggtg ccgcggccgc cgcgtgagtc tgctgcgctc catgaggaag
      121 atgcttgccg ctgtatcccg cgtgttggca ggctctgcgc agaagccggc aagccgagtg
      181 ctggttgctt cccgtaattt tgcaaatgat gctacatttg agattaagaa atgtgacctt
      241 catcggctag aagagggccc cccagtcacc acagtgctca ccagagagga tgggctcaag
      301 tactacagga tgatgcagac tgtgcgccgg atggagctaa aggcggatca gctgtataag
      361 cagaaaatca ttcgtggttt ctgtcacttg tgtgatggtc aggaagcctg ctgcgtgggc
      421 ctggaggctg gcataaaccc tacggaccac ctcatcactg cctatcgagc acatggcttc
      481 accttcactc ggggcctgcc tgtgcgagca attcttgcag agctaacagg acgaagagga
      541 ggttgtgcta aagggaaagg cgggtcaatg cacatgtacg ccaagaactt ctatggaggc
      601 aacggcatcg ttggagctca ggtgcccctg ggagcaggaa ttgccctggc ctgcaagtac
      661 aatggaaaag atgaggtctg tttgacatta tacggcgatg gtgctgctaa tcagggtcag
      721 atctttgaag cttacaatat ggcagcactg tggaaattac cttgcatttt tatctgtgag
      781 aacaaccgct atggcatggg gacgtctgtt gagagagcag cagccagcac ggactactac
      841 aaaagaggag attttattcc tggactcagg gtagatggaa tggatatctt gtgcgtccga
      901 gaggcaacaa agtttgcggc tgcctattgc aggtctggta aggggcccat cctgatggag
      961 ctccagactt accgctacca tggacacagc atgagtgacc ctggagtaag ctaccgcact
     1021 cgagaagaaa tccaggaagt aagaagtaag agtgacccta ttatgcttct caaggataga
     1081 atggtgaaca gcaatcttgc aagtgttgaa gaattaaagg agattgatgt ggaagtgagg
     1141 aaagaaatcg aggatgctgc ccagtttgcc acggctgatc ctgagccccc gttggaggaa
     1201 ctaggctatc acatctacag cagtgatcct ccctttgaag tgcgtggtgc caaccagtgg
     1261 atcaagttta agtcagtcag ttaatgggtg actgttaggt gggtggatgg gtgttcctca
     1321 tccagcgtaa ggaactctgt gctctcaact tggacaggaa atacccagac aacaaaaggc
     1381 ttgaaaacat gtaaattaag ggagagtaaa attgcatgca gtttgtaaaa aaaaaaaaaa
     1441 aaaaaa
//