From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: D.pseudoobscura Adh gene for alcohol dehydrogenase
Date: 28 Dec 92 21:27:20 GMT
Distribution: bionet
Checksum: 41388 8

LOCUS       DROADHGN46   3466 bp ds-DNA             INV       01-DEC-1992
DEFINITION  D.pseudoobscura Adh gene for alcohol dehydrogenase
ACCESSION   X62226
KEYWORDS    ADH gene; alcohol dehydrogenase.
SOURCE      Drosophila pseudoobscura DNA.
  ORGANISM  Drosophila pseudoobscura
            Eukaryota; Animalia; Metazoa; Arthropoda; Uniramia; Insecta;
            Pterygota; Neoptera; Holometabola; Diptera; Brachycera;
            Cyclorrhapha; Schizophora; Drosophiloidea; Drosophilidae.
REFERENCE   1  (bases 1 to 3466)
  AUTHORS   Schaeffer,S.W. and Miller,E.L.
  TITLE     Estimates of gene flow in Drosophila pseudoobscura determined from
            nucleotide sequence analysis of the alcohol dehydrogenase region
  JOURNAL   Genetics 132, 471-480 (1992)
  STANDARD  full automatic
COMMENT     *source: strain=MXA64; *source: isolate=Amecameca, Mexico; *source:
            chromosome=IV section 88;
               From EMBL    entry DPADHGN46;  dated 16-OCT-1992.
FEATURES             Location/Qualifiers
     mRNA            join(40..119,915..1020,1084..1488,1551..1966)
                     /note="adult mRNA (Adh)"
     mRNA            join(882..1020,1084..1488,1551..1966)
                     /note="larval mRNA (Adh)"
     promoter        8..14
                     /note="adult promoter (Adh)"
     prim_transcript 40..1966
                     /note="adult primary transcript (Adh)"
     exon            40..119
                     /note="adult exon 1a"
     intron          120..914
                     /note="adult intron"
     promoter        846..853
                     /note="larval promoter (Adh)"
     prim_transcript 882..1966
                     /note="larval primary transcript (Adh)"
     exon            882..1020
                     /note="larval exon 1"
     exon            915..1020
                     /note="adult exon 1b"
     intron          1021..1083
                     /number=1
     exon            1084..1488
                     /number=2
     intron          1489..1550
                     /number=2
     exon            1551..1966
                     /number=3
     polyA_signal    1943..1948
     polyA_site      1966
     CDS             join(928..1020,1084..1488,1551..1817)
                     /EC_number="1.1.1.1"
                     /gene="Adh"
                     /product="alcoholdehydrogenase"
                     /codon_start=1
                     /translation="MSLTNKNVVFVAGLGGIGLDTSRELVKRNLKNLVILDRIDNPAA
                     IAELKAINPKVTITFYPYDVTVPVAETTKLLKTIFAQVKTIDVLINGAGILDDHQIER
                     TIAVNYTGLVNTTTAILDFWDKRKGGPGGIICNIGSVTGFNAIYQVPVYSGSKAAVVN
                     FTSSLAKLAPITGVTAYTVNPGITKTTLVHKFNSWLDVEPRVAEKLLEHPTQTSQQCA
                     ENFVKAIELNKNGAIWKLDLGTLEPITWTQHWDSGI"
BASE COUNT      976 a    786 c    796 g    908 t
ORIGIN      
        1 cgtagtgtat ttaaggcact gcacatgtcg aggaccacga ttattgtcta cagtgcagtc
       61 agtagttgtc aggtgcagca ggcggaccag catttcttta aatctactca atcgatcaag
      121 taagtaactg taatccaagt acagtcggcc catcccactc ggttctgccg taactcaaga
      181 aatttcttaa tagcgcagtt ccagttgtaa aacgtcgttt aaaaaatcat ctcgttggag
      241 cttgggttga aaaacgttcg agttgggcgt agaaaagtta aaatataaaa tatcaaaaaa
      301 gagaaaatat cttcccaaag cagctgtcca ttttttgtta aagtgataga tcaaaaattc
      361 gtatgacgta tttcccaagt gctctttatg caaataattt cctcaattct aattctacaa
      421 gaagcacttt tggctcttgt ccagaaaaaa caatttgttt acacttttcg gttgataaca
      481 gaggcggacc gacggctaag taacataaca gtcgcctgtt aagtaatgcc ctcactctct
      541 gttagatggc cgcgcgtgtc gactccgata gaccagcaga cacgcagtag ttttcctcaa
      601 gaacctgaac tctaaacata gacataattt acgatacctt gagactcaaa taaaaataca
      661 agtatgagtg gtattctatt cctgattaga ctcgcacaca cacacgtacg agtattaaca
      721 taaatttgcc ggcaaataac caaaacaaaa cgaacgcaaa cgatccgaac acgtaatgcg
      781 agagataaga aacagaaaag ctcgacgtga aagaagcttc tggcggaagc tcttgccgaa
      841 ctccctataa atacgagaca gaaaaccaga ataaatataa taacaaactg ttgaattgtc
      901 ccatcgattc ccagatcaaa agacagaatg tctctcacca acaagaacgt cgttttcgtg
      961 gccggtctgg gcggcattgg cctggacacc agccgggagt tggtcaagcg taatctgaag
     1021 gtaagagaga acgaattcca tggaatctat ggaaatccta aatttaaaaa tgtatttttt
     1081 tagaacctgg tcatcctgga tcgcattgac aatccggctg ccattgccga actgaaggca
     1141 atcaatccca aggtgaccat caccttctat ccctacgatg tgactgtgcc cgtcgctgag
     1201 accaccaagc tcctgaagac catctttgcc caggtgaaga caatcgatgt cctgatcaac
     1261 ggtgctggca tcctggacga tcatcagatc gagcgcacca ttgccgttaa ctacacgggc
     1321 ctggtcaaca ccaccacagc cattctggac ttctgggaca agcgcaaggg cggcccaggc
     1381 ggcatcattt gcaacattgg ctccgtcacc ggtttcaatg ccatctacca ggtgcccgtt
     1441 tactccggct ccaaggcggc ggtggttaac tttacctcct ccctggcggt aagaaatcct
     1501 acaactatcc caaaatccca aaaactaaat atttctaaat attcccatag aaactggctc
     1561 ccattactgg tgtcactgct tacactgtca atcctggcat cactaagacc actctggtcc
     1621 acaaattcaa ctcgtggctg gatgtggagc cccgtgtggc ggagaagctg ctcgagcatc
     1681 ccacccagac ctctcagcag tgcgccgaga actttgtgaa ggccattgag ctgaacaaga
     1741 acggtgccat ctggaagttg gatctgggca ccttggagcc catcacatgg acccagcact
     1801 gggattcggg catctaaacc agcccagaga ctctatggga catggcgttt tagcttttag
     1861 tttctttttt tccactcaat tgttacgtat atacatacac atatggcaat aaggctgatt
     1921 tgaaccccgt tttgaatatg ataatacaaa attatatttg agaaatttca acaaaatcga
     1981 taagagctct agctctcttt taattaaaaa aaactagata aaaagagcaa tggtcagtgg
     2041 tggtcagtgg tcgttgtggc ctctatcgat ttccacacaa aaactttact gtttagtaat
     2101 agaaaagaac ttgagaaagg cagccaaaag aatgtacgat ctgacgggta agcatgtctg
     2161 ctatgtggct gactgcggtg gcattgcact ggagaccagc aaggttctca tgaccaagaa
     2221 tatagcggtg agtggtagag aggtggtgta gagagtggag agtggttctg gttcttaaac
     2281 aagtctctag tctctagtct cttggctgct gctggtagct gtcgaaacta attaagttaa
     2341 tgactttttc atgttttgtt tttgtccgct gacttttgct gttcaaagct acaattaagt
     2401 ttatgttttg agtttgatct acttttgctt aagggaagta ctttcgatta cggattgctg
     2461 gcatataaaa atagatacca aggaatgtgc atcatattga ttctatcttg attctttgcc
     2521 cagaaactgg ccatccttca gagcgtggaa aaccccccgg ccatcgctca gttacaatcg
     2581 attaagcaca gcacacagat cttcttctgg accttcgatg tgaccatggc ccgcgaggag
     2641 atgaagaagt acttcgatga ggtcatggtc cagatggact acatcgatgt gctgatcaat
     2701 ggggccaccc tgtgcgatga gcggaacatt gatgccacca tcaatacgaa tctgaccggc
     2761 atgatgaaca ccgtggctac tgtgctgccc tatatggacc gaaagatggg cggttcgggt
     2821 ggactgatcg tgaatgtcac ctccgtcata ggactggatc catcgccagt cttttgtgcg
     2881 tacagtgcct caaagtttgg tgtgattggg ttcacccgaa gtctagcggt gagtgggcga
     2941 gatcttaact tgaaaatgct ctctaatgtc tttcattctt tcgcaggatc ccctgtatta
     3001 cacccaaaat ggtgtggctg tcatggccgt ctgctgtggt cccaccaaag tgtttgtcga
     3061 tcgggaactg actgccttcc tggcatacgg ccaaaccttt gccgatcgcc tgcgtcgtgc
     3121 cccctgccag tcgacggccg tctgcggcca gaatatagta aatgccatcg agagatcgga
     3181 gaacggacag atctggattg ccgacaaggg cggcctggag tcggtggccc tccactggta
     3241 ttggcatatg gccgatcagt ttgtgaacta tatgcagagc actgacgatg aggatcagga
     3301 gttttttttg gggcagcgat gaggcatgcc ttgatccacg gaataaaaca tcaaatgtgt
     3361 cacaaaaagt aggcaacttg tttgtgggtt tggaaataga tttgaaatat tttgaggtca
     3421 tatgaataat gacataaatg agggtgcctg aaagtatgcc aagttt
//