From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Methanobacterium thermoformicicum plasmid-encoded type II
Date: 21 Jul 92 10:06:45 GMT
Distribution: bionet
Checksum: 34970     6

LOCUS       MBFTIRM      2879 bp ds-DNA             BCT       21-JUL-1992
DEFINITION  Methanobacterium thermoformicicum plasmid-encoded type II
            restriction-modification system (TIR) gene complete cds, (TIM)
            gene, complete cds.
ACCESSION   M97222
KEYWORDS    .
SOURCE      Methanobacterium thermoformicium (strain THF) DNA.
  ORGANISM  Methanobacterium thermoformicium
            Prokaryotae; Archaeobacteria; Methanobacteriales;
            Methanobacteriaceae.
REFERENCE   1  (bases 1 to 2879)
  AUTHORS   Noelling,J. and de Vos,W.M.
  TITLE     Characterization of the archaeal, plasmid-encoded type II
            restriction-modification system MthTI of Methanobacterium
            thermoformicicum strain THF: Homology to the bacterial NgoPII
            system from Neisseria gonorrhoeae
  JOURNAL   J. Bacteriol. (1992) In press
  STANDARD  simple staff_entry
FEATURES             Location/Qualifiers
     promoter        127..161
     CDS             187..1032
                     /gene="TIR"
                     /codon_start=1
                     /translation="MSNLLQAIMNIKSIQERNLGSYKGVQDSKNRMNQMGVTLEVFLK
                     DAFCNTFDIENKDLVYSEYFSYLGNQNNPPDMILKGGDAVEVKKITGIKTSIQLNSSY
                     PKSKLFVSDSRITEACKNCEDWEVKDIIYAIGTIPNRVLKLMFFVYGDCYAASPSIYQ
                     RIVEEVKGGLHSTGLEFSETNELGRINRVDPLGITDLRVRGMWIIKHPIKVFKNIIPP
                     ESIKNNNFNLIALMKAEKYKQFPKKDRKRIEAEDNIEVTDVKIKDPDNPAKLLDSVLV
                     RYDEI"
     promoter        895..932
     CDS             1029..2021
                     /gene="TIM"
                     /codon_start=1
                     /translation="MNMDIASFFSGAGGLDLGFTKAGFNIVFANDNWKGCWKTFEKNH
                     GIKINKKPIEWLKPSEIPDVVGFIGGPPCQSWSLAGSMCGADDPRGKTFYAYVDLVKE
                     KDPLFFLAENVPGIVSRTHLPEFKRLVNSFIDIGYNVEYKVLNAKDYGVPQDRKRVFI
                     VGYREDLNLKFEFPKPLNKKVTLRDAIGDLPEPKPALEKNRSNGENLEVPNHEYMTGT
                     FSSRYMSRNRVRSWDEVSFTIQAGGRHAPCHPQANKMIKVGPDKFIFDPESPKPYRRL
                     SVRECARIQGFPDDFIFYYKNVADGYTMVGNAVPVKLAEELAKKIKKDLEGVLN"
     terminator      1050..1056
     terminator      2035..2044
BASE COUNT      966 a    531 c    605 g    777 t
ORIGIN
        1 gtcaacgcag tcctcatatt caccccgata ttggctcaaa tcttcttcaa agagtctaca
       61 aatattccct gcccgcctaa ttttaaccac catagtttta ttttttttaa tatctaacta
      121 tcatagttta tttcagaaca gtctaaaaat tctaatagat gtcattgaga tgagggggta
      181 aataacatgt caaatttatt gcaagctatc atgaatataa aaagtattca agaaagaaac
      241 ttgggatcat ataagggtgt ccaagactcc aaaaatagaa tgaaccagat gggagttaca
      301 ttagaggttt tcctaaaaga tgctttctgc aatacatttg atatagaaaa caaagacctt
      361 gtgtacagtg aatacttttc ttaccttgga aatcaaaata acccccccga catgatcctg
      421 aaggggggtg acgcagttga agtcaagaag ataactggaa taaaaacttc gattcaactt
      481 aacagttcat accccaagtc aaagctcttt gtttctgaca gcagaataac agaggcttgc
      541 aagaactgtg aagattggga ggttaaagac ataatatatg ccatagggac tatcccaaac
      601 agagtcctta agttaatgtt ttttgtttat ggggactgtt acgcggcaag cccttctatc
      661 taccagagaa ttgttgaaga agtaaagggg ggtctacata gtactggcct agaatttagt
      721 gaaacaaacg agctaggtag aattaacagg gtagaccccc tgggaattac agacctaagg
      781 gtacgtggta tgtggatcat caaacacccc ataaaagtgt ttaaaaacat aatcccccca
      841 gaatcaatta aaaataataa ctttaattta attgccctga tgaaagcaga gaagtataaa
      901 caatttccaa aaaaagatag gaaaagaatt gaggcagaag ataatatcga agttaccgat
      961 gtaaaaataa aagatcctga caaccctgcc aaactattag atagcgttct tgtgaggtat
     1021 gatgaaatat gaatatggac atagcgtcat ttttttcggg tgctggaggt ctcgacttag
     1081 ggtttacaaa agcaggtttt aatattgtct ttgctaacga caattggaaa gggtgctgga
     1141 aaactttcga aaaaaaccac ggaataaaaa taaataaaaa acccattgaa tggttaaaac
     1201 cctcagaaat acctgacgtg gttggtttca tagggggacc cccatgccag agctggagcc
     1261 tggcaggttc aatgtgcggg gcagacgacc cccgtggtaa aacattttat gcatacgtgg
     1321 atttagtcaa agaaaaagat cccctatttt tcttggcaga aaacgtgcca gggatagttt
     1381 caagaacaca cctccctgaa ttcaaaagac ttgtaaactc attcatagac atagggtaca
     1441 acgtagaata taaggtgctc aacgcaaagg actacggcgt gccacaagac agaaaaaggg
     1501 tctttatcgt cgggtaccgt gaggacttaa acctaaaatt tgagtttcct aaacctttaa
     1561 acaagaaagt gaccctgagg gacgctattg gtgaccttcc tgaacccaag cccgcgcttg
     1621 aaaaaaacag gtcaaatggg gaaaacttgg aagtgccaaa ccacgaatac atgacaggga
     1681 cattttcaag caggtatatg tcaaggaaca gggtaaggag ttgggatgaa gtttcattta
     1741 caattcaagc gggggggcgc catgccccat gccacccaca ggctaataaa atgataaagg
     1801 tgggacctga caagttcata tttgatccag aaagtcctaa accctaccgc aggttatccg
     1861 tccgcgaatg cgctaggata caaggttttc ctgatgactt tatattctat tataaaaacg
     1921 ttgcagacgg ttatactatg gttggtaacg ctgttcctgt gaaattagca gaagaactag
     1981 caaaaaagat aaaaaaagat ttagagggcg ttttgaatta gaattagatt tgtgttattt
     2041 ttttaaggtc acagagagta aattgcgagt tgtaaccagg aacaatacct cttttaaggt
     2101 cctcttttaa ggtcacagag agtaaattgc gagttgtaac caggaacaat acctctttta
     2161 aggtcacaga gagtaaattg cgagttgtaa ccaggaacaa tacctcaaat ttttttcatg
     2221 agtaattttt ttcatgagcc aactagccat caagcctgca ccatttgatg tgaagtcaga
     2281 gagatactgt tcaactgcaa aaaggtattt atattataaa aaatcaaacc tttcatatat
     2341 aaaaaatttt caaggttttt tacatggcga aatacaagaa gtgttgaaca gaaagatttg
     2401 cctgcttttg ctacaaggtg tccctgttac aatgtctctg ttacaagtgt tacaagtaga
     2461 aattcactct ctgtttccca agcaatgggt tgttttttgt tacaagtaga aattcactct
     2521 ctctgtccca ccgtgggccc atgtgcttat atagaaattt ggtattaatt cttttcaatt
     2581 agaaatatat atacaataag aagttatgat tacatataga aatgaggttt cgaattgtaa
     2641 agttgtattg cggggagctg cagatgtttg agaagagacc tagacttttc aaaaacaggg
     2701 atgtcctcag cccgctcttt gtgcctgaca ccctgcagga cagaaaggaa gaggtgggtg
     2761 ctatcagcca gtacttgggg tacatcctcg acggcgccac cccaccccac ctgttgattg
     2821 tagggccccc aggatctggg aagactgtca cgacgaagta tgtgatcaac gaactcgag
//