From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Mouse perforin 1 mRNA, complete cds.
Date: 6 Nov 92 18:31:36 GMT
Distribution: bionet
Checksum: 62575 6

LOCUS       MUSPER1      2920 bp ss-mRNA            ROD       02-NOV-1992
DEFINITION  Mouse perforin 1 mRNA, complete cds.
ACCESSION   J04148
KEYWORDS    perforin.
SOURCE      Mouse cytotoxic T-lymphocyte, cDNA to mRNA, clones ps[2.9,1.8c8].
  ORGANISM  Mus musculus
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Rodentia; Myomorpha; Muridae; Murinae.
REFERENCE   1  (bases 1 to 2920)
  AUTHORS   Lowrey,D.M., Aebischer,Y., Olsen,K., Lichtenheld,M., Rupp,F.,
            Hengartner,H. and Podack,E.R.
  TITLE     Cloning, analysis, and expression of murine perforin 1 cDNA, a
            component of cytolytic T-cell granules with homology to complement
            component C9
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 86, 247-251 (1989)
  STANDARD  full automatic
COMMENT     Draft entry and computer-readable sequence for [1] kindly provided
            by D.Lowrey, 12-JAN-1989.
FEATURES             Location/Qualifiers
     sig_peptide     149..208
                     /note="putative"
                     /codon_start=1
     mat_peptide     209..1810
                     /product="perforin 1"
                     /note="putative"
                     /codon_start=1
     polyA_signal    1966..1971
     CDS             149..1813
                     /product="perforin 1"
                     /codon_start=1
                     /translation="MATCLFLLGLFLLLPRPVPAPCYTATRSECKQKHKFVPGVWMAG
                     EGMDVTTLRRSGSFPVNTQRFLRPDRTCTLCKNSLMRDATQRLPVAITHWRPHSSHCQ
                     RNVAAAKVHSTEGVAREAAANINNDWRVGLDVNPRPEANMRASVAGSHSKVANFAAEK
                     TYQDQYNFNSDTVECPMYSFRLVQKPPLHLDFKKALRALPRNFNSSTEHAYHRLISSY
                     GTHFITAVDLGGRISVLTALRTCQLTLNGLTADEVGDCLNVEAQVSIGAQASVSSEYK
                     ACEEKKKQHKMATSFHQTYRERHVEVLGGPLDSTHDLLFGNQATPEQFSTWTASLPSN
                     PGLVDYSLEPLHTLLEEQNPKREALRQAISHYIMSRARWQNCSRPCRSGQHKSSHDSC
                     QCECQDSKVTNQDCCPRQRGLAHLVVSNFRAEHLWGDYTTATEAYLKVFFGGQEFRTG
                     VVWNNNNPRWTDKMDFENVLLSTGGPLRVQVWDADYGWDDDLLGSCDRSPHSGFHEVT
                     CELNHGRVKFSYHAKCLPHLTGGTCLEYAPQGLLEILQETACGAVW"
BASE COUNT      686 a    805 c    767 g    662 t
ORIGIN      
        1 cccagtggac gaagccagtc cagctctggc atgaataact aaagagaagt tcactctctc
       61 ctgatgttcc ccagtcgtga gaggtcagca tccttcatcc ctgttcccac agctttccag
      121 agtttatgac tactgtgcct gcagcatcat ggccacgtgc ctgttcctcc tgggcctttt
      181 cctgctgctg ccacgacctg tccctgctcc ctgctacact gccactcggt cagaatgcaa
      241 gcagaagcac aagttcgtgc caggtgtatg gatggctggg gaaggcatgg atgtgactac
      301 cctccgccgc tccggctcct tcccagtgaa cacacagagg ttcctgaggc ctgaccgcac
      361 ctgcaccctc tgtaaaaact ccctaatgag agacgccaca cagcgcctac ctgtggcaat
      421 cacccactgg cggcctcaca gctcacactg ccagcgtaat gtggccgcag ccaaggtcca
      481 ctccacggag ggtgtggccc gggaggcagc tgctaatatc aataacgact ggcgtgtggg
      541 gctggatgtg aaccctaggc cagaggcaaa catgcgcgcc tccgtggctg gctcccactc
      601 caaggtagcc aattttgcag ctgagaagac ctatcaggac cagtacaact ttaatagcga
      661 cacagtagag tgtccgatgt acagttttcg cctggtacaa aaacctccac tccaccttga
      721 cttcaaaaag gcgctcagag ccctcccccg caactttaac agctccacag agcatgctta
      781 ccacaggctc atctcctcct atggcacgca ctttatcacg gctgtggacc tcggtggccg
      841 catctcggtc cttacagccc tgcgtacctg tcagctgacc ctgaatgggc tcacagctga
      901 tgaggtagga gactgcctga acgtggaggc ccaggtcagc atcggtgccc aagccagcgt
      961 ctccagtgaa tacaaagctt gtgaggagaa gaagaaacag cacaaaatgg ccacctcttt
     1021 ccaccagacc taccgtgagc gtcacgtcga agtacttggt ggccctctgg actccacgca
     1081 tgatctgctc ttcgggaacc aagctacacc agagcagttc tcaacctgga cagcctcact
     1141 gcccagcaac cctggtctgg tggactacag cctggagccc ctgcacacat tactggaaga
     1201 acagaacccg aagcgggagg ctctgagaca ggctatcagc cattatataa tgagcagagc
     1261 ccggtggcag aactgtagca ggccctgcag gtcaggccag cataagagta gccatgattc
     1321 atgccagtgt gagtgccagg attcaaaggt caccaaccag gactgctgcc cacgacagag
     1381 gggcttggcc catttggtgg taagcaattt ccgggcagaa catctgtggg gagactacac
     1441 cacagctact gaagcctacc taaaggtctt ctttggtggc caggagttca ggaccggtgt
     1501 cgtgtggaac aataacaatc cccggtggac tgacaagatg gactttgaga atgtgctcct
     1561 gtccacaggg ggacccctca gggtgcaggt ctgggatgcc gactacggct gggatgatga
     1621 ccttcttggt tcttgtgaca ggtctcccca ctctggtttc catgaggtga catgtgagct
     1681 aaaccacggc agggtgaaat tctcctacca tgccaagtgt ctgccccatc tcactggagg
     1741 gacctgcctg gagtatgccc cccaggggct tctggagatc ctccaggaaa ccgcatgtgg
     1801 ggctgtgtgg taacataata acaacaataa catgcctgag agctgggtgt agtagcacac
     1861 gcctttaatc ccagcatttg ggaggcagag acaggtggat atctatgagt tcgaggccag
     1921 cctgggtcta cagggtctca aaaaaaaaaa aatttttaaa tcttcaataa aaaccaaagc
     1981 cagagcgctc tcccacgcac agctcagtcg tcactatcag acagtgtctc atactgcgct
     2041 gagaggagcg gggcaggctc ctctccagat gcggttctgt tgatgtggag ctgcttgggt
     2101 gatggcagag gggcgcatgc gatctgtgta ggtggtgtac tgctgagcat ccgtatggtc
     2161 agagggttgt aagggaactg agtagacctg ttgaagaggg ccgatcttgc catgccctac
     2221 cttggtgtct gcctgtggta atcaccttct gaatgcacag aggagacaga agaaggcctt
     2281 tcagttccta aaatagcttt gcccaggaat aggagattta gacttcctgc tgtttgattt
     2341 gttgatcagc tttggtttgc atactcctgc atgaggtgat ggctcccctc atctctccgt
     2401 gcctcgctgc tcgtcaccac tgaggtgctg gcactaccag gcataatgcc cacaggatgg
     2461 gacatgacaa caccatgatc ttcaacttta tcatcaaaac ttcccatgag agcctttctg
     2521 atgatgtctt ctagaccaag ttactggcgg gatcagcaca agaatggctt cttgagctca
     2581 ctgctcctga tgtggtaact gctggcaatg agatctctgt cctggctgag cagccatatc
     2641 gagagtcacc tccaccaaga agagtcaact acgaaaaatt tcctgtttct ttgatttaac
     2701 catgggtgat gtgctttcaa gcttggtgaa gaatgaaggc aagtagctta tacttcctgg
     2761 tgatcgagaa tcactccttt gctcagcagg gtccactccc ctctgtgaca agagcaacat
     2821 gctgtcctgc ttctcatgca cagcagggcc ttggggtggg gagatgggct catagggctc
     2881 tgatgggata tgactcctct ctgaagattt tccaggcctg
//