From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Anabaena sp. (clones lambda-An-700 and lambda-An-710) ATP synthase
Date: 15 Sep 92 10:02:02 GMT
Distribution: bionet
Checksum: 36182    14

LOCUS       ANAATP1      7000 bp ds-DNA             BCT       15-SEP-1992
DEFINITION  Anabaena sp. (clones lambda-An-700 and lambda-An-710) ATP synthase
            a, b, b', c, delta, alpha and gamma subunit genes, complete cds.
ACCESSION   M21659
KEYWORDS    ATP synthase.
SOURCE      Anabaena sp. (library: PCC 7120) DNA.
  ORGANISM  Anabaena sp.
            Prokaryotae; Gracilicutes; Oxyphotobacteria; Cyanobacteria;
            Nostocales; Nostocaceae.
REFERENCE   1  (bases 1 to 7000)
  AUTHORS   McCarn,D.F., Whitaker,R.A., Alam,J., Vrba,J.M. and Curtis,S.E.
  TITLE     Genes encoding the alpha, gamma, delta, and four F-0 subunits of
            ATP synthase constitute an operon in the Cyanobacterium Anabaena
            sp. strain PCC 7120
  JOURNAL   J. Bacteriol. 170, 3448-3458 (1988)
  STANDARD  simple staff_entry
FEATURES             Location/Qualifiers
     mRNA            151..7000
                     /note="atp1 cluster mRNA"
     CDS             292..660
                     /product="ATP synthase subunit 1"
                     /gene="atp1"
                     /codon_start=1
                     /translation="MQEFYQLYQELVLITLVLTGVVFISVWIFYSLNIALNYLLGACT
                     GVVYLRMLAKDVERLGREKQSLSKTRLALLMALILLAARWNQLQIMPIFLGFLTYKAT
                     LIIYVVRVAFISDSPKLRQP"
     CDS             713..1468
                     /product="ATP synthase subunit a"
                     /gene="atpI"
                     /codon_start=1
                     /translation="MLNFLNFYSVPLAELEVGKHLYWQIGNLKLHGQVFLTSWFVIGV
                     LVLASVAASSNVKRIPSGIQNLLEYALEFIRDLAKNQIGEKEYRPWVPFVGTLFLFIF
                     VSNWSGALVPFKLIHLPEGELTAPTSDINTTVALALLTSLAYFYAGFSKKGLGYFGNY
                     VQPVSFMLPFKIIEDFTKPLSLSFRLFGNILADELVVAVLVLLVPLFVPLPVMAFPLF
                     TSAIQALIFATLAAAYIGEAMEDHHGEEHEEHH"
     CDS             1551..1796
                     /product="ATP synthase subunit c"
                     /gene="atpH"
                     /codon_start=1
                     /translation="MDPLVSAASVLAAALAVGLAAIGPGIGQGNAAGQAVEGIARQPE
                     AEGKIRGTLLLSLAFMEALTIYGLVVALVLLFANPFA"
     CDS             1942..2433
                     /product="ATP synthase subunit b'"
                     /gene="atpG"
                     /codon_start=1
                     /translation="MTHWITLLAVEKVAKEGGLFDLDATLPLMAIQFLLLALILNATL
                     YKPLGKAIDGRNEYVRNNQLEAQERLSKAEKLAEAYEQELAGARRQAQTIIADAQAEA
                     QKIAAEKVAAAQKEAQAQREQAAGEIEQQKQQALASLEQQVDALSRQILEKLLGADLV
                     KQR"
     CDS             2543..3106
                     /product="ATP synthase subunit b"
                     /gene="atpF"
                     /codon_start=1
                     /translation="MGTFLLLMAEASAVGGELAEGGAEGGFGLNTNILDTNLINLAII
                     ITVLFVFGRKVLGNTLKTRRENIETAIKNAEQRAADAAKQLKEAQQKLEQAQAEAERI
                     KKSAQDNAQTAGQAIIAQAAVDIERLQEAGAADLNAELDRAIAQLRQRVVALALQKVE
                     SELQGGISEDAQKTLIDRSIAQLGGGV"
     CDS             3103..3654
                     /product="ATP synthase subunit delta"
                     /gene="atpD"
                     /codon_start=1
                     /translation="MTSKVANTEVAQPYAQALLSIAKSKSLTEEFGTDARTLLNLLTE
                     NQQLRNFIDNPFIAAENKKALIKQILSEASPYLRNFLLLLVDKRRIFFLPEILQQYLA
                     LLRQLNQTVLAEVTSAVALTEDQQQAVTEKVLALTKARQVELATKVDSDLIGGVIIKV
                     GSQVIDSSIRGQLRRLSLRLSNS"
     CDS             3818..5338
                     /product="ATP synthase subunit alpha"
                     /gene="atpA"
                     /codon_start=1
                     /translation="MSISIRPDEISSIIQQQIEQYDQEVKVANVGTVLQVGDGIARIY
                     GLEKAMAGELLEFEDGTVGIAQNLEEDNVGAVLMGEGREIQEGSTVTATGRIAQIGVG
                     EALIGRVVDALGRAIDGKGDIKASESRLIESPAPGIIARRSVHEPMQTGITAIDSMIP
                     IGRGQRELIIGDRQTGKTAIAIDTIINQKGEDVVCVYVAIGQKASTVANVVQTLQEKG
                     AMDYTVVVAAGASEPATLQFLAPYTGATIAEYFMYKGKATLVIYDDLSKQAQAYRQMS
                     LLLRRPPGGEAYPGDVFYIHSRLLERAAKLSDELGKGSMTALPIIETQAGDVSAYIPT
                     NVISITDGQIFLSSDLFNAGIRPAVNPGISVSRVGSAAQTKAMKKVAGKIKLELAQFD
                     DLQAFAQFASDLDKATQDQLARGQRLRELLKQSQNQPLSVAEQVAILYAGINGYLDDI
                     PVDKVTTFTKGLRDYLKSGVNPYFQDVQSKKALGDDEEKALKAALEDYKKTFKATA"
     misc_feature    5349..5413
                     /note="pot. transcription termination signal"
                     /note="putative"
     CDS             5427..6374
                     /product="ATP synthase subunit gamma"
                     /gene="atpC"
                     /codon_start=1
                     /translation="MPNLKSIRDRIQSVKNTKKITEAMRLVAAARVRRAQEQVIATRP
                     FADRLAQVLYGLQTRLRFEDVDLPLLKKREVKSVGLLVISGDRGLCGGYNTNVIRRAE
                     NRAKELKAEGLDYTFVIVGRKAEQYFRRREQPIDASYTGLEQIPTADEANKIADELLS
                     LFLSEKVDRIELVYTRFVSLVSSRPVIQTLLPLDTQGLEAADDEIFRLTTRGGQFQVE
                     RQTVTSQARPLPRDSIFEQDPVQILDSLLPLYLSNQLLRALQESAASELAARMTAMSN
                     ASENAGELIKSLSLSYNKARQAAITQELLEVVGGAEALT"
     misc_feature    6399..6443
                     /note="pot. transcription termination signal"
                     /note="putative"
     misc_feature    6948..6978
                     /note="pot. transcription termination signal"
                     /note="putative"
BASE COUNT     2024 a   1537 c   1583 g   1856 t
ORIGIN      926 bp upstream of EcoRI site.
        1 ttacacaaca aggttgagga gcattccctg atcttgcctc tcgtatctag cttatcctga
       61 gggtaagttt ttctctaacc gtatctcctg ggcaagaagt taattgtttt tttctaaagt
      121 attgttaccg gatcgtgata tgattcagct gactgaatgt gcagtcataa taagttagct
      181 gtactggttt caagtcccgt gagcttgtca gaagaaccaa tttcacccac cccgacaaca
      241 cgacaagata ctcaacctgg ttttgaggac gcagaaccag caaactcttc catgcaggag
      301 ttttatcaac tctatcaaga gttggtgtta atcactcttg tcttaacagg ggttgtattt
      361 atctctgtct ggatctttta ttccttaaac attgctctga attatttatt aggagcgtgt
      421 acaggtgtgg tttacttgag gatgttggca aaagatgttg agcgtttagg tagagaaaaa
      481 cagtcgctga gtaaaactcg gttggcgtta ttaatggcgc tgattttgct ggcagcacgg
      541 tggaatcaat tgcaaataat gcccatcttt ttgggatttc tcacctacaa agcgacgctc
      601 atcatttatg tagttagggt ggcttttatc tctgactcgc caaagctccg gcaaccttaa
      661 aaggctcctc ttctccaggt aagattggag aagcaattga aatggaagga aaatgttgaa
      721 ttttctgaac ttttactctg ttccacttgc cgaattggaa gtgggaaaac atctgtactg
      781 gcaaataggc aacttaaaac tgcacggtca ggtgtttctc acctcttggt ttgttattgg
      841 cgtgctagta ctagcttctg tggctgcaag cagtaacgtt aaacggattc ctagtggcat
      901 acagaaccta ctggagtatg ccctggaatt cattcgggat ttggctaaaa accagattgg
      961 cgaaaaagaa tatcgcccct gggtgccttt cgttggcact ttgtttttgt tcatttttgt
     1021 gtcaaattgg tcaggagcct tagttccctt caagctgatt catttgcctg aaggagaatt
     1081 aacagcacca accagcgaca tcaatacaac tgttgcatta gctttgttga catccttggc
     1141 gtatttttac gctggattca gcaaaaaagg actggggtat tttggtaact acgtgcaacc
     1201 tgtatcgttc atgttgccat tcaaaattat tgaagacttc actaagcccc tttccctgag
     1261 tttccgttta ttcggtaaca tcttagctga tgaacttgta gtggccgtac tggtattact
     1321 agtgccttta tttgtacctt tgccagtaat ggctttccca ctatttacaa gcgctatcca
     1381 ggcactaatt tttgctactt tggctgcggc ttacatcggt gaagcgatgg aagatcatca
     1441 tggcgaagag catgaggaac atcattagtc attagttatt tagttaagta acaactgaca
     1501 accgaccact aacaaaacca ttcgtttcat taaagtaagg aaagaatatc atggatccat
     1561 tagtttctgc tgcttccgtt ttagctgctg ctctagctgt tggtttggct gcaatcggcc
     1621 ctggtattgg tcaaggtaat gcagcaggac aagctgtaga aggtattgcc cgtcagcccg
     1681 aagcagaagg taaaattcgc ggtacattac tactcagttt ggcgttcatg gaagcgctaa
     1741 ccatctacgg tctagtagtt gctctagtat tgttgtttgc taaccccttc gcataatcag
     1801 tactgagtac tgggtgctga gtgctgagtg ttgagtgaaa atagttagga gttaagggtt
     1861 taagccttta acccataact tataactcac aactcacaac tcacaactca ggactaacat
     1921 agtagatagg aacaagcaaa catgacacat tggatcacct tattggcggt ggaaaaggtt
     1981 gccaaggaag gcgggctatt tgatttagat gctaccttac ccttaatggc aatccagttc
     2041 ctcttgttag ctctgatatt gaatgccact ctctacaagc cattgggtaa agctattgat
     2101 gggcgaaatg aatatgttcg taacaatcaa ttagaagccc aagagcgttt gtccaaagct
     2161 gagaaattgg cagaggctta cgaacaagag ttagcaggag ctagacggca agcacaaaca
     2221 attattgctg acgctcaagc cgaagcccaa aaaattgctg ctgaaaaagt agcagcagcg
     2281 caaaaagaag ctcaggcaca aagagaacaa gcggccggtg aaattgagca gcaaaaacag
     2341 caggctctgg cttccttaga gcaacaagta gatgccctaa gtcgccaaat cttagaaaag
     2401 cttttgggag ccgatctagt aaagcagcgc taactcaata aatttagtca ttagtcattg
     2461 gtcattagtc attagtcaaa agagagaatg acgaagggcg aatgacacaa acctttggca
     2521 gcgcagttgt ggatgaaaaa tcatggggac ttttttactg ttgatggcgg aagccagcgc
     2581 cgttggaggt gaattggcag aaggtggggc ggaaggtggt tttggtttaa ataccaatat
     2641 tctcgacacc aacctgatta acctggccat tattattact gtgctgtttg tctttggacg
     2701 gaaggttttg ggtaataccc tgaaaactcg ccgcgaaaac attgaaacag caattaagaa
     2761 tgcagaacaa cgtgccgcag atgcagccaa gcaactgaaa gaggcgcaac aaaaactaga
     2821 gcaagcacaa gcagaagctg agagaatcaa aaaatcagcc caagacaatg ctcaaaccgc
     2881 aggtcaagcc atcattgccc aagctgctgt agatattgaa cgcttacaag aagcaggggc
     2941 agcagacttg aatgcagaac tagatagagc gatcgctcag ttgcggcagc gagtagttgc
     3001 tttggcattg caaaaggtcg aatcagaact gcaaggtggc attagtgaag atgctcaaaa
     3061 aactctaatt gaccgtagca tcgcacaatt gggaggcgga gtatgacaag taaagtagca
     3121 aacactgagg tagctcaacc ttacgctcag gcactgttgt caatcgccaa atcgaaaagc
     3181 ttgacggaag agttcggcac agatgcgcgt actttgctga acctgctgac agaaaatcaa
     3241 cagctacgca acttcatcga taaccccttt attgcagctg agaacaaaaa agctctcatc
     3301 aaacaaatat tgagtgaagc tagcccttac ctacgtaact tcttactgtt gttggtagat
     3361 aaacgacgca ttttcttctt gccagagatt ttacagcagt atttggctct cttacggcaa
     3421 ctgaatcaaa ccgtattagc ggaagttact tctgctgtgg ctttaacaga agatcaacag
     3481 caagcagtta cagaaaaggt attggcactc accaaagctc gtcaagtgga actggcaacc
     3541 aaggtagaca gtgacctgat tggtggtgtg atcattaaag taggctctca ggtaattgac
     3601 tctagtatcc ggggtcagtt gcgtcgcctc tccttgcgct taagcaatag ctagaaagtt
     3661 cagaggttaa agttagcggt ttcacatcag gcacttgaca attaaaattg tttgtgattt
     3721 gtggttaact cttaacacct caaactaaat gcagacgcga tattgtttct cgcgtctatt
     3781 aaatatcttc cgtctcgcaa gaaaaaaaga tacacacatg agcatttcaa ttagacctga
     3841 cgaaatcagc agtattattc agcagcaaat cgagcaatac gaccaagaag tcaaagttgc
     3901 taacgtcggt actgtactac aagtaggtga cggtatcgcc cggatctatg gtctagaaaa
     3961 ggctatggct ggggaacttt tggaatttga agacggcaca gttggtatcg cccaaaactt
     4021 agaagaagat aacgttggtg cggtactgat gggtgaaggc cgggaaattc aagaaggtag
     4081 caccgttact gctactggca gaattgctca aatcggtgtc ggtgaagcct taattggccg
     4141 cgttgtcgat gctttgggtc gtgctattga cggtaaaggc gacatcaaag ccagcgaaag
     4201 ccgcttgatt gaatctcctg cgcctggtat catcgctcgt cgttccgtac acgaaccgat
     4261 gcaaaccggt atcactgcga ttgactccat gattcctatt ggccgtggtc aacgggaatt
     4321 aatcattggt gaccgtcaaa caggtaaaac tgcgatcgcg atcgacacca tcattaacca
     4381 aaaaggtgaa gatgtagttt gcgtttacgt tgccattggt caaaaagctt ctaccgttgc
     4441 taacgtagtc caaactctgc aagaaaaagg tgcaatggat tacacagtag tagttgctgc
     4501 tggtgccagt gaacctgcta ccctacaatt cctggctccc tacacaggcg ctactattgc
     4561 tgagtacttc atgtacaaag gcaaagctac cttggtaatt tacgacgact tatctaagca
     4621 agctcaagct tatcgccaaa tgtccttgct gttacgtcgt ccaccaggag gcgaagctta
     4681 ccctggagac gtattctaca ttcactctcg tttgttggaa agagcagcaa aactcagtga
     4741 tgaactcggt aaaggtagca tgaccgcgct accaatcatc gaaacccaag ctggtgacgt
     4801 ttctgcctac attcctacca acgtaatttc tattacagac ggtcagatat tcttatcttc
     4861 tgacttattt aacgctggta ttcgtccggc tgtaaaccct ggtatctctg tatcccgtgt
     4921 gggttctgcg gcacaaacca aagcgatgaa aaaagttgct ggtaagatta agctcgaact
     4981 agcacagttt gacgacctcc aagccttcgc gcaatttgct tccgacctag ataaagccac
     5041 ccaagaccaa ttggcaagag gtcaacgcct gcgggaactc ctcaaacagt cccaaaatca
     5101 gcctctatcc gtagctgaac aagtagccat tctgtacgca ggtatcaacg gttacttaga
     5161 tgatatccct gttgataaag tcaccacctt caccaaaggc ttgagagatt acttgaagtc
     5221 cggcgttaac ccctacttcc aagacgtaca atcgaagaaa gcactgggtg atgatgaaga
     5281 aaaagcattg aaggcagctt tagaagacta caaaaagacc ttcaaagcta cagcgtaatt
     5341 agtcattagt cattggtcag tggtcagtag caatagacac tgacaactga caactgacaa
     5401 ctgacaactg acaatcaaca aatattatgc ctaatctcaa atcaatacgc gatcgcattc
     5461 agtcggtcaa aaacaccaag aaaatcacag aagccatgcg gctggtagcg gcggcgcgtg
     5521 tacgtcgcgc ccaagaacaa gtaatcgcta ctcgtccttt tgctgaccgt ttggcacaag
     5581 tattatacgg tttgcaaact cgtctacggt ttgaagatgt agacttacct ctactgaaaa
     5641 aacgggaagt taaatcagta ggcttgctag ttatttctgg cgatcgtggt ttgtgcggcg
     5701 gttacaatac caacgtcatc cgtcgtgccg aaaaccgcgc caaggaactc aaggcagaag
     5761 gtctagatta cacatttgtt attgttgggc ggaaagctga acaatatttt agacggcgtg
     5821 agcaacccat cgatgctagt tatactggct tagagcaaat ccccaccgcc gatgaagcta
     5881 ataaaattgc tgacgaattg ctctctttat tcctctcaga aaaagtagac cgcatcgagt
     5941 tagtttacac ccgcttcgtc tccttggtta gctctcgtcc cgtcatccaa accttgctac
     6001 cccttgacac tcaaggttta gaagcagccg atgacgaaat attccgtctg acaactcgtg
     6061 gcggtcaatt ccaagtggaa cgccaaacag tgaccagcca agctcgccct ttgccccgcg
     6121 acagtatttt tgaacaagac ccagtacaga ttcttgattc actattgccc ttgtatttaa
     6181 gcaaccagct attacgggcg ctgcaagagt cagccgctag tgaattagcc gcgcggatga
     6241 cagcaatgag caacgctagt gaaaacgctg gtgaattaat caagtctctt tcactgtcct
     6301 acaacaaagc ccgtcaagcc gccattaccc aagaacttct tgaggttgtt ggtggtgcag
     6361 aagcgttgac ttaggaaaag ccaattgcta gacatgacaa atagctaatt gctagtcact
     6421 ttcaacttgg caattagcta tttttaattt ttaggtgttc ccgttaacca tcctatgatt
     6481 acaaattacg aatgacgaac tacgaattaa aaatcccgta ctataatgag aatataaaga
     6541 tcatgggtcc gtcggtttcg acaggttggc gaacgctact ctgtgattca ggtcgagagt
     6601 gagtctcctc tgcaaatcaa ggctcaaaac aaaagtaaat gcgaataaca tcgttaaatt
     6661 tgctcgtaag gacgctctag tagctgccta aatagcctct ttcaggttcg agcgtcttcg
     6721 gtttgactcc gttaaggact gaagaccaac ccccaacgga tgctctagca atgttctctg
     6781 gttggcttgc tagctaagat ttaatcagag catcctacgt tcgggataat gaacgattcc
     6841 cgccttgagg gtcagaaagg ctaaacctgt gaatgagcgg ggggtcaata cccaatttgg
     6901 acagcagttc gactctgctc gatccactaa cagtaaatat agttaacaag tccacttcct
     6961 acgaggtgga ctttttgatt tatggataca tttcctctgg
//