From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Pseudomonas putida phenol hydroxylase (dmpKLMNOP) operon, complete
Date: 22 Dec 92 22:46:52 GMT
Distribution: bionet
Checksum: 23444 11

LOCUS       PSEPHHYD     5449 bp ds-DNA             BCT       23-NOV-1992
DEFINITION  Pseudomonas putida phenol hydroxylase (dmpKLMNOP) operon, complete
            cds.
ACCESSION   M60276 M37764 M37775 M57564
KEYWORDS    phenol hydroxylase.
SOURCE      Pseudomonas putida (strain CF600) plasmid DNA.
  ORGANISM  Pseudomonas putida
            Prokaryotae; Gracilicutes; Scotobacteria; Pseudomonadaceae.
REFERENCE   1  (bases 1 to 5449)
  AUTHORS   Nordlund,I., Powlowski,J. and Shingler,V.
  TITLE     Complete nucleotide sequence and polypeptide analysis of
            multicomponent hydroxylase from Pseudomonas sp. strain CF600
  JOURNAL   J. Bacteriol. 172, 6826-6833 (1990)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     CDS             746..1024
                     /gene="dmpK"
                     /note="putative"
                     /product="phenol hydroxylase"
                     /codon_start=1
                     /translation="MTVTNTPTPTFDQLTRYIRVRSEPEAKFVEFDFAIGHPELFVEL
                     VLPQDAFVKFCQHNRVVAMDEAMAKAVDDDMVKWRFGDVGRRLPKDPG"
     CDS             1077..2072
                     /gene="dmpL"
                     /evidence="EXPERIMENTAL"
                     /product="phenol hydroxylase"
                     /codon_start=1
                     /translation="MSVEIKTNTVDPIRQTYGNLQRRFGDKPASRYQEASYDIEAVTN
                     FHYRPLWDPQHELHDPTRTAIRMTDWHKVTDPRQFYYGAYVQTRARMQEATEHAYGFC
                     EKRELLSRLPAELQAKLLRCLVPLRHAELGANMNNSSIAGDSIAATVTQMHIYQAMDR
                     LGMGQYLSRIGLLLDGGTGEALDQAKAYWLDDPIWQGLRRYVEDSFVIRDWFELGLAQ
                     NLVLDGLLQPLMYQRFDQWLTENGGSDVAMLTEFMRDWYGESTRWVDAMFKTVLAEND
                     ANREQVQAWLEVWEPRAYEALLPLAEEATGIAALDEVRSAFATRLQKIGLKSREE"
     CDS             2076..2348
                     /gene="dmpM"
                     /evidence="EXPERIMENTAL"
                     /product="phenol hydroxylase"
                     /codon_start=1
                     /translation="MSSLVYIAFQDNDNARYVVEAIIQDNPHAVVQHHPAMIRIEAEK
                     RLEIRRETVEENLGRAWDVQEMLVDVITIGGNVDEDDDRFVLEWKN"
     CDS             2360..3913
                     /gene="dmpN"
                     /evidence="EXPERIMENTAL"
                     /product="phenol hydroxylase"
                     /codon_start=1
                     /translation="MATHNKKRLNLKDKYRYLTRDLAWETTYQKKEDVFPLEHFEGIK
                     ITDWDKWEDPFRLTMDTYWKYQAEKEKKLYAIFDAFAQNNGHQNISDARYVNALKLFL
                     TAVSPLEYQAFQGFSRVGRQFSGAGARVACQMQAIDELRHVQTQVHAMSHYNKHFDGL
                     HDFAHMYDRVWYLSVPKSYMDDARTAGPFEFLTAVSFSFEYVLTNLLFVPFMSGAAYN
                     GDMATVTFGFSAQSDEARHMTLGLEVIKFMLEQHEDNVPIIQRWIDKWFWRGYRLLTL
                     IGMMMDYMLPNKVMSWSEAWGVYFEQAGGALFKDLERYGIRPPKYVEQTTIGKEHITH
                     QVWGALYQYSKATSFHTWIPGDEELNWLSEKYPDTFDKYYRPRFEFWREQQAKGERFY
                     NDTLPHLCQVCQLPVIFTEPDDPTKLSLRSLVHEGERYQFCSDGCCDIFKNEPVKYIQ
                     AWLPVHQIYQGNCEGGDVETVVQKYYHIKSGVDNLEYLGSPEHQRWLALKGQTPPTAA
                     PADKSLGAA"
     CDS             3981..4340
                     /gene="dmpO"
                     /evidence="EXPERIMENTAL"
                     /product="phenol hydroxylase"
                     /codon_start=1
                     /translation="MTVNSIGEYTATPRDVQANFNGMQLLYLYWEEHLMYCSALAFLV
                     APGMPFAEFLEQVLKPAIHAHPDSAKIDFSQALWQLNDQPFTPDYAASLEANGIDHKS
                     MLRLNTPGLNGIQGSCS"
     CDS             4351..5412
                     /gene="dmpP"
                     /evidence="EXPERIMENTAL"
                     /product="phenol hydroxylase"
                     /codon_start=1
                     /translation="MSYNVTIEPTGEVIEVEDGQTILQAALRQGVWLPFACGHGTCAT
                     CKVQVVEGEVDIGEASPFALMDIERDERKVLACCAIPLSDLVIEADVDADPDFLGHPV
                     EDYRGVVSALVDLSPTIKGLHIKLDRPMPFQAGQYVNLALPGIDGTRAFSLANPPSRN
                     DEVELHVRLVEGGAATGFIHKQLKVGDAVELSGPYGQFFVRDSQAGDLIFIAGGSGLS
                     SPQSMILDLLERGDTRRITLFQGARNRAELYNCELFEELAARHPNFSYVPALNQANDD
                     PEWQGFKGFVHDAAKAHFDGRFGGQKAYLCGPPPMIDAAITTLMQGRLFERDIFMERF
                     YTAADGAGESSRSALFKRI"
BASE COUNT     1157 a   1690 c   1611 g    991 t
ORIGIN      
        1 gagctcggtg gggcggacct tgaccagacc cttgagtgaa tgcatctgcg gcccagcgag
       61 gaacatgccg acttcgctgg cattcggtct cagcttcctg gccagttcgg catccttcag
      121 gccggactgg taaccatggc gcaggaacaa gcccttggcg cgttcgatgc ccagggtatt
      181 gaccatttcc cggcgaaagc tggccatcgc tgaaaactgc agcaacagca tgcgctgttc
      241 gccaagccag atcttgcctt ccatgctctg gaagtggatc aggttggtca ggtccttgaa
      301 atcggagtgc tggatttcag gcttgtactt gatcggcatg taagcgaggc ccctatttat
      361 ttttagatgg ggaaaatcag ggtcgccgct atagcgcaag gcaggcggcg attccagatg
      421 gggtcatggg gaaaatcggc agttttttca cctgcgccag ctccccatag ccagcctcag
      481 tatctggcaa aagtcaacca aatgatcaat cgacggcagt gattttagta ttagagatca
      541 gcgttcagct gcgccataag catttgctca agcggccttg ggcaattgat caaatgctta
      601 aaaagtctgc gcaagcgcgg cttaatttcg ctcgctccga tcattctaaa aattagaaac
      661 acattgaaaa acaatacctt gaagtctgtt ttcagacctt ggcacagccg ttgcttgatg
      721 tcctgcgcaa gccgccaacc tggagatgac cgtgaccaat acccccacac cgactttcga
      781 tcagctcact cgttacatcc gtgtgcgcag cgaaccagaa gccaagttcg tcgagttcga
      841 tttcgccatt ggtcatccgg agctgttcgt cgagttggtg ctgccgcaag acgccttcgt
      901 gaagttttgc cagcacaacc gcgtggtggc aatggacgaa gcgatggcca aggcggtgga
      961 cgacgacatg gtcaagtggc gcttcggcga tgtcggtcgc cgcttgccga aagacccggg
     1021 ctgagaaccc tgccgacagg cagatgggca tccaacaaca agagggtacg gttgatatga
     1081 gcgtagagat aaagaccaat acggtggatc cgatccgcca gacctacggc aacctgcaac
     1141 ggcgcttcgg cgacaagccg gctagccgtt atcaggaagc cagctacgac atcgaagcgg
     1201 tcaccaactt tcactatcgc ccgctgtggg acccgcagca cgagctgcac gatccgaccc
     1261 gcacggcgat ccgcatgacc gattggcaca aggtcaccga cccccgccaa ttctactacg
     1321 gcgcctatgt gcagacccgc gcgcggatgc aggaagccac cgaacacgcc tatggcttct
     1381 gcgaaaagcg tgagctgctg agccgtctgc cggccgagtt gcaggccaag ctgctgcgct
     1441 gcctggtgcc gctgcggcat gccgagctgg gcgccaacat gaataacagc agcatcgccg
     1501 gcgacagcat cgccgccacc gtgacccaga tgcacatcta ccaggcgatg gaccgcctgg
     1561 gcatgggcca gtacctctcg cgcatcggcc tgctgctcga tggcggcacc ggcgaggcgt
     1621 tggatcaagc caaggcctat tggctcgacg acccgatctg gcagggcctg cgtcgctacg
     1681 tcgaagacag cttcgtgatc cgcgactggt tcgagttggg cctggcgcag aacctggtgc
     1741 tcgacggctt gctgcagccg ctgatgtacc agcgcttcga ccaatggctc acagagaacg
     1801 gtggcagcga tgtggccatg ctcaccgagt tcatgcgcga ctggtacggc gaaagcacgc
     1861 gctgggtcga cgccatgttc aagaccgtgc ttgccgaaaa tgacgctaac cgtgagcagg
     1921 tgcaggcctg gctggaggtc tgggagccgc gtgcctacga ggcattgttg cccctggccg
     1981 aggaagccac cggtatcgcc gcgctggatg aagtccgcag cgccttcgct actcgcctgc
     2041 agaaaatcgg cctgaaaagc cgcgaggaat aaagcatgtc atcactcgtc tacatcgcct
     2101 tccaggataa cgacaacgcg cgttacgtgg tggaagcgat catccaggac aacccccacg
     2161 ccgtcgtcca gcaccacccg gcgatgatcc gtatcgaggc cgagaagcgc ctggagatcc
     2221 gcagggaaac cgtggaagag aacctcggcc gcgcctggga cgtccaggaa atgctggtgg
     2281 acgtaatcac catcggcggc aacgtcgacg aggacgatga ccgcttcgtc ctcgagtgga
     2341 agaactagga gacaagctca tggctaccca caacaagaaa cgcctcaacc tgaaagacaa
     2401 ataccgctac ctgacccgcg atctggcctg ggaaacgacc taccagaaga aagaagacgt
     2461 gttcccgctg gagcacttcg agggcatcaa gatcaccgac tgggacaagt gggaagaccc
     2521 cttccgcctg accatggaca cctactggaa ataccaggcg gagaaagaga agaagctcta
     2581 cgcgatcttc gacgcctttg cccagaacaa tggtcatcag aacatttccg atgcgcgcta
     2641 cgtcaacgcc ctgaagctgt tcctcaccgc cgtttcaccg ctggaatacc aggccttcca
     2701 gggcttctcg cgggttggcc ggcagttcag tggcgccggt gcgcgggtcg cctgtcagat
     2761 gcaggcgatc gacgagctgc gccatgtgca gacgcaagtc cacgccatga gccattacaa
     2821 caagcacttc gatggtttgc atgacttcgc ccacatgtac gaccgggtct ggtacctctc
     2881 ggtacccaag tcctatatgg acgatgcgcg gaccgccggt ccgttcgagt tcctcaccgc
     2941 cgtctcgttc tccttcgagt acgtgctgac caacctgttg ttcgtaccct tcatgtccgg
     3001 tgccgcctac aacggcgata tggccacggt caccttcggt ttctccgcgc agtcggacga
     3061 ggcgcggcac atgaccctgg gtctggaagt gatcaagttc atgctcgaac agcatgaaga
     3121 caacgtgccc atcatccagc gctggatcga taagtggttc tggcgcggtt accgcctgct
     3181 gaccctgatc ggcatgatga tggactacat gctgccgaac aaagtgatgt cctggtctga
     3241 ggcctggggg gtctacttcg agcaggccgg tggcgcgctg ttcaaggatc tggagcgcta
     3301 tggcatccgg ccgccgaaat acgtcgagca gaccaccatc ggcaaggagc acatcaccca
     3361 ccaggtgtgg ggggccttat atcaatacag caaggccacc agcttccata cctggatacc
     3421 cggcgacgag gaactgaact ggctgtcgga gaaatacccg gacaccttcg acaaatacta
     3481 ccgcccgcgc ttcgagttct ggcgtgagca gcaggccaag ggtgagcgct tctacaacga
     3541 caccctgccg cacctctgcc aggtgtgcca gttaccggtg attttcaccg agccggacga
     3601 tccgaccaag ctcagcctgc gcagcctggt gcacgagggg gagcgctatc aattctgctc
     3661 ggatggctgc tgcgacatct tcaagaacga gccggtgaag tacatccagg cctggctgcc
     3721 ggtgcaccag atctaccagg gcaactgcga aggcggggat gtcgaaacgg tggtgcagaa
     3781 gtactaccac atcaaaagcg gcgtggacaa tttggagtac ctgggctcgc ccgagcacca
     3841 gcgctggctg gccctgaaag gtcagacccc accaactgcc gccccggcgg acaagagcct
     3901 gggcgccgcc tgaggcagcg ccagccgctc aggggtgaag caccgcccct gagccattcc
     3961 aagaacaaga ggtttcgatc atgactgtca actcaatcgg cgaatacacc gccacgccac
     4021 gggatgtgca ggccaacttc aacggcatgc aactgctcta cctctactgg gaagagcacc
     4081 tgatgtactg ctccgcgctc gcgttcttgg tagcccccgg catgcccttt gccgagttcc
     4141 tcgagcaggt gctcaagccc gcgatccacg cccatccgga cagcgcgaag atcgatttca
     4201 gccaggcgct ctggcagctg aacgaccagc cgttcacccc ggactacgcc gccagcctgg
     4261 aagccaacgg catcgaccac aaaagcatgc tgcgtctgaa caccccgggc ctgaacggca
     4321 tccagggttc gtgcagctga gaggtgtgtc atgagttaca acgtcaccat tgaaccgacc
     4381 ggcgaagtga tcgaagtgga ggacggccag accatcctcc aggccgctct gcgccagggc
     4441 gtctggctgc cgttcgcctg cggccacggc acctgcgcca cctgcaaggt gcaggtggtc
     4501 gagggcgaag tggatatcgg cgaagcctcg ccgttcgccc tgatggacat cgagcgcgac
     4561 gagcgcaagg tgctggcctg ctgcgccatt ccgctgtccg acctggtgat cgaagccgac
     4621 gtcgatgccg acccggactt cctcggccat ccggtggagg attaccgggg ggtggtcagc
     4681 gccctggttg acctgtcgcc gaccatcaag ggcctgcaca tcaagctgga tcggcccatg
     4741 ccgttccagg ccgggcagta cgtcaacctg gcattgccgg gcatcgacgg cacccgcgcc
     4801 ttctcgctgg ccaacccgcc gagccggaac gacgaagtcg agttgcacgt gcgcctggtc
     4861 gagggcggtg cggccaccgg ctttatccac aagcaactga aagtcggcga cgcggtggag
     4921 ctgtccgggc cttatgggca gttcttcgtg cgcgattcgc aggccggcga cctgatcttc
     4981 atcgccggcg gctcgggctt atcgagcccg cagtcgatga tcctcgatct gcttgaacgc
     5041 ggcgatacgc ggcggatcac cctgttccag ggcgcgcgca accgcgccga gctgtacaac
     5101 tgcgaactgt tcgaggaact ggccgcgcgc caccccaact tcagttacgt gccggcactc
     5161 aaccaggcca acgacgatcc cgaatggcag ggtttcaagg gcttcgtcca cgacgccgcc
     5221 aaggcgcatt tcgacggccg cttcggcggg cagaaagcct acctgtgcgg cccaccgccg
     5281 atgatcgacg cggccatcac caccctgatg caaggtcgct tgttcgagcg cgacatcttt
     5341 atggagcgct tctacaccgc cgccgatggg gccggcgaga gcagccgttc ggccctgttc
     5401 aagcgcatct gaggtgaacc atgaaccgtg ccggttatga gattcgcga
//