From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: E. coli DNA fragment of a putative lac transposon.
Date: 29 Dec 92 21:26:08 GMT
Distribution: bionet
Checksum: 30758 4

LOCUS       ECOLAC01     1430 bp ds-DNA             BCT       14-DEC-1992
DEFINITION  E. coli DNA fragment of a putative lac transposon.
ACCESSION   X00941 K02334
KEYWORDS    lac operon; transposon.
SOURCE      Escherichia coli DNA.
  ORGANISM  Escherichia coli
            Prokaryotae; Gram-negative facultatively anaerobic rods;
            Enterobacteriaceae.
REFERENCE   1  (bases 1 to 1430)
  AUTHORS   Buvinger,W.E., Lampel,K.A., Bojanowski,R.J. and Riley,M.
  TITLE     Location and analysis of nucleotide sequences at one end of a
            putative lac transposon in the Escherichia coli chromosome
  JOURNAL   J. Bacteriol. 159, 618-623 (1984)
  STANDARD  full automatic
COMMENT     From EMBL    entry ECLAC01;  dated 07-NOV-1985.
               When the E. coli genome is hybridized with that of S.
            typhimurium, loops corresponding to regions of non-homology appear.
             One such loop includes the lac operon of E. coli.  [1] suggests
            that E. coli may have obtained the lac operon from a transposon.
            The beginning of non-homology has been localized to a region
            spanning 13 bp between bases 523 and 535 of the sequence reported
            here; non-homology continues through the rest of this sequence and
            through the lac operon.
               This region lies between the proA and lacA genes.  The 3' end of
            this sequence is about 3.3 kb from the 3' end of the lacA cds.
FEATURES             Location/Qualifiers
     misc_feature    1..1430
                     /note="lac-loop fragment"
     misc_feature    523..535
                     /note="lac-junction sequence"
BASE COUNT      344 a    384 c    392 g    310 t
ORIGIN      5 bp upstream of PstI site; 8.0 min on K12 map
        1 ctgcaggacg gaaaaatcag cgccattgat gcgcaatccg gcgtgatgcc cataactgaa
       61 aacagcctgg atgccgaaca aggtttagtt ataccgccgt ttgtggagcc acatattcac
      121 ctggacacca cgcaaaccgc cggacaaccg aactggaatc agtccggcac gctgtttgaa
      181 ggcattgaac gctgggccga gcgcaaagcg ttattaaccc atgacgatgt gaaacaacgc
      241 gcatggcaaa cgctgaaatg gcagattgcc aacggcattc agcatgtgcg tacccatgtc
      301 gatgtttcgg atgcaacgct aactgcgctg aaagcaatgc tggaagtgaa gcaggaagtc
      361 gcgccgtgga ttgatctgca aatcgtcgcc ttccctcagg aagggatttt gtcgtatccc
      421 aacggtgaag cgttgctgga agaggcgtta cgcttagggg cagatgtagt gggggcgatt
      481 ccgcattttg aatttacccg tgaatacggc gtggagtcgc tgcataaaac cttcgccctg
      541 gcgcaaaaat acgaccgtct catcgacgtt cactgtgatg agatcgatga cgagcagtcg
      601 cgctttgtcg aaaccgttgc tgccctggcg caccatgaag gcatgggcgc gcgagtcacc
      661 gccagccaca ccacggcaat gcactcctat aacggggcgt atacctcacg cctgttccgc
      721 ttgctgaaaa tgtccggtat taactttgtc gccaacccgc tggtcaatat tcatctgcaa
      781 ggacgtttcg atacgtatcc aaaacgtcgc ggcatcacgc gcgttaaaga gatgctggag
      841 tccggcatta acgtctgctt tggtcacgat gatgtcttcg atccgtggta tccgctggga
      901 acggcgaata tgctgcaagt gctgcatatg gggctgcatg tttgccagtt gatgggctac
      961 gggcagatta acgatggcct gaatttaatc acccaccaca gcgcaaggac gttgaatttg
     1021 caggattacg gcattgccgc cggaaacagc gccaacctga ttatcctgcc ggctgaaaat
     1081 gggtttgatg cgctgcgccg tcaggttccg gtacgttatt cggtacgtgg cggcaaggtg
     1141 attgccagca cacaaccggc acaaaccacc gtatatctgg agcagccaga agccatcgat
     1201 tacaaacgtt gaacgactgg gttacagcga gcttagttta tgccggatgc gcggtgaacg
     1261 ccttatccgg cctacgtaga gcactgaact cgtaggcctg ataagcgtag cgcatcaggc
     1321 aattccagcc gctgatctgt gtcagcggct accgtgattc attcccgcca acaaccgcgc
     1381 attcctccaa cgccatgtgc aaaaatgcct tcgcagcggc tgtctgccag
//