From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Bacillus subtilis operon encoding spaB, spaC, spaD, spaE and spaS
Date: 22 Dec 92 22:42:07 GMT
Distribution: bionet
Checksum: 08065 16

LOCUS       BACSPAA      7656 bp ds-DNA             BCT       23-NOV-1992
DEFINITION  Bacillus subtilis operon encoding spaB, spaC, spaD, spaE and spaS
            genes, complete cds's; ORF, complete cds.
ACCESSION   M99263
KEYWORDS    spa operon; spaB gene; spaC gene; spaD gene; spaE gene; spaS gene;
            subtilin.
SOURCE      Bacillus subtilis (strain ATCC 6633) DNA.
  ORGANISM  Bacillus subtilis
            Prokaryotae; Firmicutes; Endospore-forming rods and cocci;
            Bacillaceae.
REFERENCE   1  (bases 1 to 7656)
  AUTHORS   Hansen,J.N. and Chung,Y.J.
  TITLE     Determination of the sequence of spaE and identification of a
            promoter in the subtilin (spa) operon in Bacillus subtilis
  JOURNAL   J. Bacteriol. 174, 6699-6705 (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     terminator      621..661
                     /standard_name="rho-independent terminator"
                     /note="putative"
                     /citation=[1]
     -35_signal      683..688
                     /note="spa operon promoter"
                     /evidence="EXPERIMENTAL"
                     /citation=[1]
     -10_signal      709..714
                     /note="spa operon promoter"
                     /evidence="EXPERIMENTAL"
                     /citation=[1]
     mRNA            831..7298
                     /function="encodes SpaE, SpaD, SpaB, SpaC, and SpaS"
                     /evidence="EXPERIMENTAL"
     CDS             1..612
                     /note="product and function unknown"
                     /citation=[1]
                     /partial
                     /note="putative"
                     /codon_start=1
                     /translation="EFGRHFLMSVYGVLFAAVVGVPVGILIAHYRRLSAWVFAVTNVI
                     QTIPALAMLAVLMLVMGLGANTVIISLFLYSLLPIIRNTYTGIVSIEHAYLESGKAMG
                     MTKFQVLRMVELPLALSVIMAGLRTALVIAIGITAIGTFVGAGGLGDMIVRGSNATNG
                     TAIILAGATPTAVMAIGADLIMAWIERFLNPVKQKSRRKVISV"
     CDS             831..3386
                     /standard_name="SpaE protein"
                     /gene="spaE"
                     /evidence="EXPERIMENTAL"
                     /citation=[1]
                     /note="SpaE protein"
                     /codon_start=1
                     /translation="MKSLYTPTDYYMIRVPLVHQDLKNENSQDIDQLLHDLCNDSLFR
                     EQILVSSRTLYETIHTFLQAPDKLKGKKKRNFQQAILKYATRRATRTTPFGLFSSVGI
                     GSFSDKNHLSFNQHSFYKKARVDFEWLYQLIRKLENEYTDRLSFTLNSACYIKGDRAY
                     LLYSTDGKSEEVSVRATSVFYLINELCGESAAYQDIIRCLIDNYPNTPINKINQYVAD
                     LIDKEFLISNLRPPMTVSDQFQYLIDQAESRHIPNELIQACKNIQYQIDAYNRITIGE
                     GEHQYLNLIETMNKLIKASSPLQVDAGAGDSSIQLDNETSLAISELASMFTYMASPSA
                     NTLDHLEKYKNVFLERYGYEREVPLLEMLCSSTGIGAPATYTNPANEFFEETSFGEQF
                     SPEMKQFFMRKYFESVRKKAPIQLDDETFHRICNSEIADEEIPLSFELNFFVKLRNGR
                     VKLYLGPNVGSTRAGKTFGRFSHMSDSISEIIKTLHNKEKELTECNTKVCELSIVPNQ
                     TRSGNVTRNVSYREKEMSLFTNSALHLNDSVKAEDILIGINKDHIFYARHKTTGEILS
                     FESNHMFNPLLMTNAVRLLLEISRDGKRKWNDFPWFSIYSDFKYIPEIKYKEITLSCE
                     QWLIYKNDLSMHSNASLEEIKSAFFEFHRTYELPQTFYIVNADNRLLIDIENDCTLDV
                     FFWELKKTNHNQPLQLVAVEHDADALMDRNQNDYSGEIVVPLLRKQPVKPLYLPVLNA
                     IEGSGSDRIKMPFEDWLFIKLYCKQTREEELIAFEIADFYNQISDQYPVRHFFMRYRD
                     PKPHIRLRFNGKAEVLYSLFPQLLNWLKSLREKGLVSESVITQYEREIERYGG"
     CDS             3392..3922
                     /function="may participate in secretion of subtilin"
                     /gene="spaD"
                     /evidence="EXPERIMENTAL"
                     /note="SpaE protein"
                     /codon_start=1
                     /translation="MEAAEQLFCEDSKVVEMMIRMHRMKDITISKEIAGMVSVIQFLE
                     QFELTFEEQLTFLERNSLQNEYRTEFKKDREMYIEICNSDRDWDNLKKTSDGGMLYET
                     LKTRKMAAAHYAFLIKKAFDNKDEVYSRIGSIIHLHCNRLFGTDRELENKILTLCRHS
                     LYAQRYQKMNGSLAWK"
     CDS             3958..5757
                     /function="subtilin secretion"
                     /standard_name="SpaB protein"
                     /note="this protein homologous to HlyB, which participates
                     in the secretion of hemolysin protein in E.coli"
                     /gene="spaB"
                     /evidence="EXPERIMENTAL"
                     /note="SpaB protein"
                     /codon_start=1
                     /translation="MKQMPKTFKLIFTLERSLFLKLIAFSIITGILPIVSLYISQELI
                     NSLVTIRKDVSIVITIFLTYLGVSFFSELISQISEFYNGKFQLNIGYKLNYKVMKKSS
                     NLALKDFENPEIYDKLERVTKEISYKPYQIIQAIITMTTSFVTLLSSIAFLMSWNPKV
                     SLLLLVIPVISLFYFLKIGQEEFFIHWKRAGKERKSWYISYILTHDFSFKELKLYNLK
                     DYLLNKYWDIKKSFIEQDTKILRKKTLLNLIYEIAVQLVGAVIIFIAIMSAFAGKIMV
                     GNVMSYIRSVSLVQNHSQSIMTSIYSIYNSNLYMNQLYEFLELKEEKSQGHKKPIVEP
                     IHSVVFQNVSFIYPNQGEQTLKHINVSLHKGERVAIVGPNGSGKSTFIKLLTGLYEVQ
                     QGDILINGINIKELDMDSYMNQIAALFQDFMKYEMTLKENIGFGQIDKLHQTNKMHEV
                     LDIVRADFLKSHSSYQFDTQLGLWFDEGRQLSGGQWQKIALARAYFREASLYILDEPS
                     SALDPIAEKETFDTFFSLSKDKIGIFISHRLVAAKLADRIIVMDKGEIVGIGTHEELL
                     KTCPLYKKMDESENYMNPLEEEGSKWKEALYQG"
     CDS             5730..7055
                     /standard_name="SpaC protein"
                     /gene="spaC"
                     /note="SpaC protein"
                     /codon_start=1
                     /translation="MERGTVSRIEVEIVKEMARQISNYDKVLEIVNQKDNFRSIGEVP
                     LIPWKSTALSHGIPGICMLYGELHAHFPEEGWDDIGHQYLSILVNEIKEKGLHTPSMF
                     SGAAGIGLAAICLSQRFTYYNGLISSINEYLAETVPQLLTEFDQRQVCMSDYDVIEGV
                     SGIANYLLLFQEDKAMGDLLIDILKYLVRLTEDIIVDGEKVPGWHIPSQHQFTDIEKK
                     AYPYGNFNMGLAHGIPGPICVLSSALIQGIKVKGQERAIEKMANFLLEFSEKEQDSLF
                     WKGIISFEEYQYGSPPNAVNFSRDAWCYGRPGVCLALVKAGKALQNTELINIGVQNLR
                     YTISDIRGIFSPTICHGYSGIGQILLAVNLLTGQEYFKEELQEIKQKIMSYYDKDYIF
                     GFHNYESMEGDEAVPLQYVGLLDGAVGVGLGVLNMELGSKTDWTKALLI"
     CDS             7130..7300
                     /standard_name="SpaS protein"
                     /note="subtilin precursor peptide"
                     /gene="spaS"
                     /evidence="EXPERIMENTAL"
                     /note="SpaS protein"
                     /codon_start=1
                     /translation="MSKFDDFDLDVVKVSKQDSKITPQWKSESLCTPGCVTGALQTCF
                     LQTLTCNCKISK"
BASE COUNT     2546 a   1267 c   1546 g   2297 t
ORIGIN      
        1 gaattcggcc gccactttct catgtcggtt tacggcgtat tgtttgccgc tgttgtcggg
       61 gttccggtcg gaatcctcat tgcgcattat cgccgcttat cagcctgggt gttcgccgta
      121 acaaatgtta tccagacaat cccggcgctc gcgatgctgg ccgttttaat gcttgtcatg
      181 gggctcggcg caaacaccgt gattatatca ttatttctgt attccctcct gccgattatc
      241 cgaaacacgt acacaggcat tgtcagcatt gaacatgcct acttagaatc gggaaaagca
      301 atgggcatga cgaaattcca ggttctccgg atggttgagc tcccgctcgc gctttctgtc
      361 attatggcag gcttgcgcac ggctctcgtc attgccatcg gaattacagc aatcggcaca
      421 tttgtcggcg ccggggggct cggggatatg attgtccgcg gctcaaacgc gacaaacgga
      481 actgcgatta ttctcgcagg cgcgaccccg actgcggtaa tggccatagg agcagatttg
      541 ataatggcct ggattgaaag gttcttgaat ccggtgaaac aaaaaagcag aagaaaggta
      601 ataagtgtat agagttaagc aaaaaggatt cttttctgag agggaaaaga gtcctttttt
      661 tatggtattt actgggtgga tcttgatatt tttttgattt ttagaatgta tagtaaaaat
      721 agagtattgt aaatattttg gttcaaatag aataatatgg aggactaagc agagggatgt
      781 agaattgttt ttattaagaa gcggataaat atggggggct atagactaat atgaaatcct
      841 tatatacacc tacagattat tatatgattc gggttccttt agtacatcaa gacttaaaaa
      901 atgagaattc tcaggatatc gatcagttat tacatgacct ttgcaacgat tcattatttc
      961 gggaacaaat actggtatct agcaggacac tatatgaaac aatacatact ttcctgcaag
     1021 cgccggataa attaaaaggg aaaaaaaagc gcaactttca acaggctatt ttgaagtatg
     1081 caacaagaag agcaacaaga accacacctt ttggcctttt ttcttcagtc ggtatagggt
     1141 cgttttctga taaaaatcac ttatctttta atcaacattc attttacaaa aaggctcgtg
     1201 ttgattttga gtggctctac caattaatta gaaaattgga aaacgaatac accgaccgac
     1261 tttcttttac attaaattcc gcttgttata ttaagggtga ccgggcttac ttgttgtaca
     1321 gcacagatgg aaaatctgaa gaagttagtg ttcgtgcgac atctgttttc tatttgataa
     1381 atgaactgtg tggtgaatct gctgcatatc aagatataat ccgttgtttg atagataact
     1441 atccaaatac tccaataaat aaaattaatc agtacgtagc agaccttatt gacaaagagt
     1501 tccttatatc aaacttacgg ccgccgatga ctgtttcaga tcaatttcag tatttaattg
     1561 atcaagcgga aagccgccat attccaaatg aactcattca ggcttgtaag aacattcaat
     1621 atcagataga tgcatataat cggatcacta tcggagaggg agagcatcag tatttaaatc
     1681 tgattgaaac aatgaataaa ctcataaagg catcatctcc tctgcaagta gacgctgggg
     1741 ctggagactc ctccattcaa ttagataatg aaacatctct cgccataagt gaattggcaa
     1801 gcatgtttac ttatatggct tctccctctg ccaatacatt agaccacttg gaaaaataca
     1861 aaaatgtatt tttggaacgc tatggatatg aaagagaagt tcctctctta gaaatgctat
     1921 gttccagcac tggcattggt gctcctgcta cgtacacgaa tcctgctaat gaattctttg
     1981 aagaaacatc atttggggag caattttcac cggaaatgaa acaatttttc atgagaaagt
     2041 attttgaatc agttagaaag aaagctccta ttcaattaga cgacgaaaca ttccatagaa
     2101 tttgcaactc tgaaattgct gatgaggaaa ttccattatc atttgaactc aatttctttg
     2161 ttaaattacg aaatgggaga gttaagcttt atttaggccc caacgtcgga tctacccgcg
     2221 cagggaaaac atttgggaga ttttctcata tgtctgattc aatcagtgaa atcataaaga
     2281 ccttacataa caaggagaaa gagttaacag agtgtaatac gaaagtttgt gaactgagta
     2341 ttgtgcctaa tcaaactagg tctggaaatg taacaagaaa tgtaagctac cgagaaaaag
     2401 agatgtctct ttttacgaac agtgctctgc atctcaatga ttccgtcaaa gctgaagata
     2461 ttctaattgg aatcaataaa gaccatatct tttatgctag acataaaaca actggcgaaa
     2521 ttctgtcttt tgagtcaaat catatgttta atcctttatt aatgaccaac gctgtacggc
     2581 ttttattaga gatttcaaga gatggtaaaa gaaaatggaa tgatttcccg tggtttagta
     2641 tctatagtga tttcaagtat attcctgaaa tcaaatataa agagataacc ttatcttgtg
     2701 aacaatggct gatatacaaa aatgatttaa gcatgcactc gaatgcatct ctagaagaga
     2761 taaaatctgc tttttttgaa tttcatcgta cttatgaact gccgcaaaca ttttatatcg
     2821 ttaacgcaga caatcgatta ttgattgata tagagaatga ttgtactttg gatgtttttt
     2881 tctgggaatt gaaaaaaacg aaccataacc agccattaca acttgtggct gttgagcatg
     2941 atgcagatgc gttaatggat agaaaccaaa atgactattc gggagaaatc gtcgttccgc
     3001 tgcttagaaa acaaccagta aaaccattgt atttaccggt tcttaacgca atagagggaa
     3061 gtggttccga cagaataaaa atgccatttg aagactggct gtttattaag ctttattgta
     3121 aacaaacaag agaagaagag ctaattgctt ttgaaatagc ggatttttat aaccagattt
     3181 ctgatcaata tccagtcaga catttcttta tgaggtatcg ggatccaaag cctcatataa
     3241 gacttagatt taatggaaaa gccgaagtgc tgtacagctt gtttccccaa ttattgaatt
     3301 ggctgaaaag cttaagagaa aaaggactgg tttcagagtc tgttatcact caatacgagc
     3361 gggagataga acgatatggc ggctaagcct tatggaggct gcagaacagc ttttctgtga
     3421 agacagcaaa gttgttgaaa tgatgattag aatgcaccgg atgaaagata ttacgataag
     3481 caaggaaatt gcaggcatgg tttcggttat acagttttta gaacagttcg agctaacgtt
     3541 tgaagaacag ttaacttttt tagagagaaa ttccttacag aatgagtatc gtactgaatt
     3601 taaaaaggat agagaaatgt atattgaaat atgcaattct gacagagatt gggataatct
     3661 caagaaaaca agtgatggcg gtatgttata tgaaactttg aaaacaagaa aaatggctgc
     3721 agctcattat gcatttttaa tcaaaaaggc atttgataac aaagatgaag tttattcacg
     3781 tataggaagt atcatccatc tgcattgcaa tcgtttattc ggaaccgaca gagaactgga
     3841 aaataaaatt ctcaccctat gcagacattc tttatatgcg caacgatatc aaaagatgaa
     3901 tggtagttta gcatggaagt aaaggaacaa ctgaaactaa aagagctgct gtttatcatg
     3961 aaacaaatgc ctaagacgtt caagttgatt ttcaccttag aaagatcact gtttttaaag
     4021 ttgattgcat tcagtattat taccggtatt ctgccaattg tttcactata tatttcacaa
     4081 gaactgatta attcccttgt gactatccgg aaagatgttt caattgttat caccattttc
     4141 ttgacatatc taggggtatc ttttttttcg gagctcattt cgcagatttc cgaattttat
     4201 aatggtaaat ttcaattaaa tattggttat aaactcaact ataaagtcat gaaaaagagc
     4261 agtaatttag ctctcaagga ttttgaaaat ccagaaatat atgacaagtt agagagggta
     4321 acgaaagaga tcagttataa gccctatcag attattcaag ccatcattac aatgaccaca
     4381 tcatttgtga cgctactctc atcaattgcg tttttaatgt cgtggaaccc taaagtctca
     4441 cttctgttgt tagttattcc ggtaatttct ctattctatt tcttgaaaat tggacaggaa
     4501 gaattcttca tacactggaa acgcgcggga aaggaaagga aatcttggta tatcagctat
     4561 atactcacac atgatttttc ttttaaagaa ttaaaattgt ataaccttaa ggattattta
     4621 ttaaacaagt actgggatat caaaaaatca tttatagaac aagatacaaa gatattaaga
     4681 aagaaaactt tattgaacct aatatatgag attgcagtgc aattagtcgg agctgtcatt
     4741 atatttattg ccatcatgtc tgcttttgcg ggaaaaataa tggtcggtaa tgtaatgagt
     4801 tatatcagat cggtttcgct ggtgcaaaat cactcacaat ctatcatgac aagcatttat
     4861 tcgatctata acagcaatct ctatatgaat caattgtatg aatttctgga attaaaagaa
     4921 gagaaaagtc aaggtcacaa gaagccgatt gttgagccta ttcattctgt tgtttttcaa
     4981 aatgtcagct ttatctatcc caatcaagga gaacagacac tgaaacatat taacgtttca
     5041 ttgcataaag gagaacgtgt agccattgtt ggaccaaacg gatcagggaa aagtacattc
     5101 attaagctcc ttaccggctt atatgaagtt cagcagggcg acattttgat taatggaata
     5161 aatattaaag aactggatat ggacagttat atgaatcaaa ttgcagcgct atttcaagac
     5221 tttatgaaat acgaaatgac attaaaagaa aatataggat tcggccaaat tgataaacta
     5281 catcaaacaa acaaaatgca tgaagttctc gatattgtga gagccgattt cttaaaaagc
     5341 cactcttcct atcaattcga tacacagctc ggactatggt ttgatgaagg aagacagcta
     5401 tccggtggac aatggcaaaa gattgctttg gcaagggctt attttaggga agcttctttg
     5461 tatattttgg acgagccaag ttctgcgctg gatccaattg cagaaaaaga aaccttcgat
     5521 acttttttca gtttgtcaaa agataaaata ggaatattta tttctcatcg attggttgct
     5581 gctaaacttg cagatcggat cattgttatg gataaaggag agattgtagg aataggcaca
     5641 catgaagagc tattaaaaac atgtccgctg tataaaaaaa tggatgaatc cgagaactat
     5701 atgaacccac tggaggagga aggcagtaaa tggaaagagg cactgtatca aggatagaag
     5761 ttgaaattgt gaaagaaatg gctcgtcaaa tttctaatta cgacaaagtg cttgaaatag
     5821 tgaatcaaaa agataacttt cgcagcattg gcgaagttcc gcttatacct tggaaatcaa
     5881 ctgctttgag tcatggtata ccaggcattt gtatgctgta tggcgaattg cacgctcatt
     5941 ttcctgagga aggatgggac gacataggac atcaatattt atcgatttta gtaaatgaaa
     6001 taaaagaaaa agggctgcat actccttcaa tgttttctgg agcagctggc attggactgg
     6061 cagcaatctg tttatcgcaa cgtttcacct attacaatgg tttgatttct agtattaatg
     6121 aatatttggc tgaaaccgta cctcaattgt taactgaatt tgatcagcgg caagtgtgta
     6181 tgagtgacta cgatgtcata gaaggagtta gcggaattgc caactatctg ctgttatttc
     6241 aagaagataa agctatggga gatttgttaa ttgatatttt gaaatatctc gttaggttaa
     6301 ctgaagacat tatcgtggat ggagaaaaag ttcctggatg gcatattcca tctcaacatc
     6361 agtttactga tattgaaaaa aaagcttatc cttacggcaa ttttaatatg ggattagcac
     6421 atgggatacc tggtcctatt tgtgttctct cttcagcact catacaggga attaaagtaa
     6481 agggacaaga acgtgcgatt gaaaaaatgg cgaatttcct actggaattt tctgaaaaag
     6541 agcaagacag cttgttttgg aaaggaatca taagctttga ggagtatcaa tacggatctc
     6601 cgccaaacgc agtaaatttt agcagagatg cttggtgtta tggaagacct ggtgtatgtc
     6661 tagcgcttgt taaagccgga aaagcacttc aaaatactga acttataaat atcggagtac
     6721 agaatttaag atacacaata tctgatatac ggggaatttt ttcacctacc atatgtcatg
     6781 gttacagcgg tatcggtcag attctgcttg ctgtaaatct actcaccgga caggagtatt
     6841 ttaaggaaga gcttcaagag attaaacaaa agatcatgag ctactatgac aaggattata
     6901 tctttggatt ccataactat gaatcaatgg aaggggacga agcagtacct ttgcagtacg
     6961 ttggtttgtt ggatggagct gtaggtgtag gcttaggggt attaaacatg gaattaggct
     7021 caaaaacaga ttggacaaaa gcattattaa tttaataaaa aaaggaaaaa aatgataaaa
     7081 tcttgatatt tgtctgttac tatttaggta ttgaaaggag gtcaccaata tgtcaaagtt
     7141 cgatgatttc gatttggatg ttgtgaaagt ctctaaacaa gactcaaaaa tcactccgca
     7201 atggaaaagt gaatcacttt gtacaccagg atgtgtaact ggtgcattgc aaacttgctt
     7261 ccttcaaaca ctaacttgta actgcaaaat ctctaaataa gtaaaaccat tagcatcacc
     7321 ttgctctgac tccttgcact tctgagtgtt atacatactt attttcatag agtcgggaca
     7381 agaaaatgaa gtaaaaaacg acgggtgtga aagagtttat attcacaccc gtttttatat
     7441 tcggctttaa ggaggaacac aattgtagaa cggaagaacg gttattttcg atcatgcgtt
     7501 ttgaataaca ttccaataaa aattccagtc tcttcctcaa atgcagacaa aggatgaagg
     7561 acttaagggt acttaccagg ttttatggtt aagaatattt ctaagaacat catatttttt
     7621 attaggaaat taataaatga gattgatcac tctaga
//