From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Bacteriophage phiCTX (isolated from Pseudomonas aeruginosa) cosR,
Date: 29 Dec 92 21:37:46 GMT
Distribution: bionet
Checksum: 63511 6

LOCUS       BPHCOSR      2413 bp ds-DNA             PHG       15-DEC-1992
DEFINITION  Bacteriophage phiCTX (isolated from Pseudomonas aeruginosa) cosR,
            attP, int genes.
ACCESSION   D13409
KEYWORDS    integrase.
SOURCE      Bacteriophage phiCTX (isolated from Pseudomonas aeruginosa) DNA.
  ORGANISM  Bacteriophage phi-CTX
            Viridae; Nonclassified viruses.
REFERENCE   1  (bases 1 to 2413)
  AUTHORS   Hayashi,T., Matsumoto,H., Ohnishi,M. and Terawaki,Y.
  TITLE     Molecular analysis of a cytotoxin-converting phiCTX of Pseudomonas
            aeruginosa: structure of attP-cos-ctx region and integration into
            the serine tRNA gene
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
COMMENT     Submitted (15-Oct-1992) to DDBJ by: Tetsuya Hayashi Department of
            Bacteriology Shinshu University School of Medicine 3-1-1 Asahi
            Matsumoto 390 Japan Phone:  0263-35-4600 x5202 Fax:
            0263-33-6458.
FEATURES             Location/Qualifiers
     misc_feature    2393..2413
                     /note="This feature applies to a gene which lacks a coding
                     region feature. "
                     /gene="cosR"
     RBS             228..232
     terminator      complement(380..395)
     RBS             438..442
     RBS             complement(638..643)
     promoter        complement(668..697)
     terminator      1626..1653
     misc_feature    1737..1766
                     /note="core sequence of attP"
     misc_feature    2393..>2413
                     /gene="cosR"
     CDS             complement(<395..628)
                     /product="ORF2"
                     /codon_start=1
                     /translation="VSAVAEDSLLDLVVDDAGQPFGIGYILGRCVPWYGLPTQLALVA
                     EVDPDPFSAYLDSVCHLVPLIHTKRSGYQASKK"
     CDS             240..347
                     /product="ORF1"
                     /codon_start=1
                     /translation="MHELLKMLDSPRSLLNFSLATLAVLALYFVVVTGR"
     CDS             449..1618
                     /gene="int"
                     /product="integrase"
                     /codon_start=1
                     /translation="MADGVEVRGKRIRIYFRYQGELCRESIPGDATPENIANAERLAG
                     IINYEIKQGVFSYSRHFPDSPRVKSNTLGHYIDLWLDIKRNQIAASGFRGYTSRVETH
                     IRPRWGDSQADSIDHLDIQDWVQNTLMPKLHNKTVREIVSNLRQIFRLYRTRNRSAHD
                     PTDGIVITLPDADDPDPFTREEIDLILGTETARIGELNLAEFMIWSGPRVSEAIALAW
                     EDVDLDTGTVVFRRARVRSQYKVTKTRRSTRKVQLLAPALRALQQQAKLTRRLPPVQI
                     EVIDRDNRTRKPQRVRFVFHNSASGAAYSTSDTLRNGWWHGHLRNAGVRSRGPNQCRH
                     TFASQMLSSGIATPEWIADQMGHTSTAMIFKHYAKWISKDGPDIVGLLNQALKLS"
BASE COUNT      574 a    697 c    672 g    470 t
ORIGIN      Right end of phiCTX phage genome.
        1 gtcgaccccg aagagcagtt ggtcctcgac ggcctgagcg acagcgactg gcgcgagcgg
       61 atcggcaatg cggttccgcc ggctgcggcc gaggccatcg ccggcgtgat gggcaccacc
      121 ctgttgttgg ctgaggctgg cgagaccttc ctgctcagta gcacgccgat ctgggtgcgt
      181 caggtcgccg ttggactgag tatttcagaa cctgctggga gtctctgagg agaaacaaca
      241 tgcacgaact attgaagatg ttagacagcc cgaggagcct gctgaacttc tcgctggcaa
      301 ccctggccgt cctggccttg tacttcgtag tggtgaccgg tcggtagaga gctaccagaa
      361 acaggcaacg aatcttcttg gcccgcttgc gggcctattt tttggaggct tggtagcctg
      421 aacgcttggt gtggatcagg ggaaccagat ggcagacgga gtcgaggtac gcggaaaacg
      481 gatcaggatc tacttccgct accagggcga gctgtgtagg gagtccatac caggggacgc
      541 aacgcccgag aatatagcca atgccgaacg gctggccggc atcatcaact acgagatcaa
      601 gcaaggagtc ttcagctaca gccgacactt cccggactca ccccgggtga aaagcaacac
      661 cttgggccat tacatcgacc tctggctcga tatcaaacgc aaccagattg ccgccagcgg
      721 cttccgcggc tacaccagcc gcgtggaaac ccacatccga cctcgctggg gtgacagcca
      781 ggccgacagc atcgaccacc tggatattca agactgggtg cagaacacgc tcatgcccaa
      841 gctgcacaac aagacggtgc gagagatcgt cagcaacctg cggcagatct tccggctgta
      901 tcgaactcgg aaccggagcg cgcacgaccc aacagacggc atcgtgatca ctcttccgga
      961 tgcggatgac ccagacccat tcactcgaga ggaaatcgac ctcatcctcg gtaccgaaac
     1021 cgcgcgcatc ggtgagctga acctggcgga attcatgatc tggagcggcc cccgcgtcag
     1081 cgaggccata gctctggcct gggaagatgt cgatttggac acggggaccg tggttttccg
     1141 ccgcgctcgt gtacgcagcc agtacaaggt caccaagact cgccgatcga cgaggaaggt
     1201 ccagctactg gccccggcgc tgcgcgcact gcagcagcag gccaagctaa ccagacgcct
     1261 tccgcccgta cagatcgagg tgatagaccg cgacaaccgg accagaaagc ctcaacgggt
     1321 acgcttcgtg ttccacaaca gtgctagcgg ggcggcgtac tccacgtcgg atacgctgcg
     1381 caacggctgg tggcatggcc acctcaggaa tgcgggcgtc cgctcgcgtg gtcccaatca
     1441 gtgccgccac accttcgcca gccagatgct cagcagcggc atcgccactc ccgaatggat
     1501 cgcggaccag atggggcaca cctcgacggc gatgatcttc aagcattacg ccaagtggat
     1561 cagcaaggac ggccccgaca tcgtaggtct gctgaatcag gcgctgaaac tttcctgaag
     1621 caacgaaaaa ggggccctgc ggggcccctt ttttctgcgc ctcgttccca gtttgttccc
     1681 aaatcgttca ctcctgagag tgggtcgaat ggaaacatca atgaaaacag caggttatat
     1741 ggcggaggcg gtgagattcg aactcatatg aaacgcccca acctcgctgg aggccgcatt
     1801 attcctgggc cctactgtcc attttcactc gcgtttgttc ccagagtgtt cccagcgtgt
     1861 gcgagagagc ctggaagcct gccgtcaaac ctgccgtccc agggcaacag ccggatttca
     1921 tgttgaccca ttgaaatggg gtaattttgg taatctctct ttgaagaatg aatttttacc
     1981 tttaatttca atagcttatg aaaaaccctt aaaggtaata atatggtaat tttgaggtaa
     2041 tcgaattacc tttctcgaag gtaagctcct caattcacca tccccttaaa aatcaataag
     2101 ttacagaaaa attacctttc caattaccct aaattacctt tcaaggtaat cgctgaaagc
     2161 caggaaccac gcggccttca gtcatcttca actgtcaaat taccaaaatt accttttccc
     2221 aaggggtcga gagaaaacca ggcaaggaga aggctttggc cgctcccaga aaagcacctg
     2281 ctctgcaggg tttcgcaggg attcatcgcc gcagaaagcc accagcagcg cccgccaagc
     2341 ctgctggctg gggtccgcag aaagtgcaga aaaagaggtc tatttagagc ggaggcgtgg
     2401 cggggggacg act
//