From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: E.coli tdk gene for thymidine kinase
Date: 25 Jul 92 10:03:15 GMT
Distribution: bionet
Checksum: 20414     4

LOCUS       ECOTDKG      1095 bp ds-DNA             BCT       25-JUL-1992
DEFINITION  E.coli tdk gene for thymidine kinase
ACCESSION   X53733 X51523
KEYWORDS    tdk gene; thymidine kinase.
SOURCE      Escherichia coli DNA.
  ORGANISM  Escherichia coli
            Prokaryotae; Gram-negative facultatively anaerobic rods;
            Enterobacteriaceae.
REFERENCE   1  (bases 1 to 1095)
  AUTHORS   Bockamp,E.O., Blasco,R. and Vinuela,E.
  TITLE     Escherichia coli thymidine kinase: Nucleotide sequence of the gene
            and relationships to other thymidine kinases
  JOURNAL   Gene 101, 9-14 (1991)
  STANDARD  full staff_review
REFERENCE   2  (bases 1 to 1095; 1 to 1095; 1 to 1095)
  AUTHORS   Black,M.E. and Hruby,D.E.
  TITLE     Nucleotide sequence of the Escherichia coli thymidine kinase gene
            provides evidence for conservation of functional domains and
            quaternary structure
  JOURNAL   Mol. Microbiol. 5, 373-379 (1991)
  STANDARD  full automatic
COMMENT     catalyses the phosphorylation of thymidine. Enzyme activity can be
            detected in tk-minus cell lines
            From EMBL 27   entry ECTDKG;  dated 29-APR-1991.
            strain=W3110    clone=4D8 and 3D5 chromosome=minute 27.4;
            See <X51523> for sequence of strain K-12.
FEATURES             Location/Qualifiers
     -35_signal      243..248
     -10_signal      265..270
     RBS             301..305
     CDS             316..933
                     /EC_number="2.7.1.21"
                     /product="thymidine kinase"
                     /gene="tdk"
                     /codon_start=1
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVDSLTAIQERHRHD"
BASE COUNT      312 a    220 c    249 g    314 t
ORIGIN
        1 gaattcccta ttaagcgaat gatgaaaagt agaacagtcg caataagagc atggacttag
       61 tattgcacta tctcctggag gtcaacagag ggctattact tgcgcaacag gttaaagatt
      121 gtgaatagtt accagcagtc atttacccgc ttataacaag cgaggcagtt gtaatgatag
      181 ctcagaagga ttatgcaagg cttcgtaagg gagaacgcat atacccactt ctgtgcatac
      241 tgttgagctg aaaaactgac gaattatgat aaactccagc caactttatt tcatatcatt
      301 gagggcctgt ggctgatggc acagctatat ttctactatt ccgcaatgaa tgcgggtaag
      361 tctacagcat tgttgcaatc ttcatacaat taccaggaac gcggcatgcg cactgtcgta
      421 tatacggcag aaattgatga tcgctttggt gccgggaaag tcagttcgcg tataggtttg
      481 tcatcgcctg caaaattatt taaccaaaat tcatcattat ttgatgagat tcgtgcggaa
      541 catgaacagc aggcaattca ttgcgtactg gttgatgaat gccagttttt aaccagacaa
      601 caagtatatg aattatcgga ggttgtcgat caactcgata tacccgtact ttgttatggt
      661 ttacgtaccg attttcgagg tgaattattt attggcagcc aatacttact ggcatggtcc
      721 gacaaactgg ttgaattaaa aaccatctgt ttttgtggcc gtaaagcaag catggtgctg
      781 cgtcttgatc aagcaggcag accttataac gaaggtgagc aggtggtaat tggtggtaat
      841 gaacgatacg tttctgtatg ccgtaaacac tataaagagg cgttacaagt cgactcatta
      901 acggctattc aggaaaggca tcgccacgat taataagaat ttctttactg acagggtgag
      961 cagggcactt ttatcctgtc agttcgtttt acgcacttct tccgggctat atacccttct
     1021 cggcagtttt ttaacgccgc tatacgcctc acagggctct taagcaccga cgttgacttg
     1081 tgacctgtaa agtac
//