From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: E.coli gltX gene encoding glutamyl-tRNA synthetase, complete cds.
Date: 15 Sep 92 10:02:54 GMT
Distribution: bionet
Checksum: 55601     5

LOCUS       ECOGLTX      1514 bp ds-DNA             BCT       15-SEP-1992
DEFINITION  E.coli gltX gene encoding glutamyl-tRNA synthetase, complete cds.
ACCESSION   M13687
KEYWORDS    synthetase; transfer RNA synthetase; transfer RNA-Glu synthetase;
            transfer RNA-aminoacyl synthetase.
SOURCE      E.coli (K12 strain N99) DNA, clone pLQ76111-delta-NruI.
  ORGANISM  Escherichia coli
            Prokaryotae; Gram-negative facultatively anaerobic rods;
            Enterobacteriaceae.
REFERENCE   1  (bases 1 to 1514)
  AUTHORS   Breton,R., Sanfacon,H., Papayannopoulos,I., Biemann,K. and
            Lapointe,J.
  TITLE     Glutamyl-tRNA synthetase of Escherichia coli: Isolation and primary
            structure of the gltX gene and homology with other aminoacyl-tRNA
            synthetases
  JOURNAL   J. Biol. Chem. 261, 10610-10617 (1986)
  STANDARD  full staff_review
COMMENT     The 'atcgat' site (nucleotide 256 to 261) was resistant to ClaI
            digestion because of the methylation of the second 'a' by the dam
            methylase (McClelland, Nucleic Acids Res. 9, 5859-5866 (1981)).  A
            rho-independent terminator is located at positions 1435-1461.
            Draft entry and sequence in computer readable form for [1] kindly
            provided by R.Breton, 04-NOV-1986.
FEATURES             Location/Qualifiers
     CDS             1..1416
                     /note="glutamyl-tRNA synthetase"
                     /gene="gltX"
                     /codon_start=1
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
BASE COUNT      379 a    369 c    414 g    352 t
ORIGIN      132 bp upstream of Sau3A site.
        1 atgaaaatca aaactcgctt cgcgccaagc ccaacaggct atctgcacgt tggcggcgcg
       61 cgtactgctc tttactcctg gctttttgca cgtaaccacg gcggtgagtt cgtgctgcgt
      121 attgaagaca ccgatcttga gcgttccacg ccggaagcta tcgaagccat tatggatggc
      181 atgaactggc tgagcctgga gtgggatgaa ggtccgtact accagaccaa acgttttgat
      241 cgctacaacg cggtgatcga tcagatgctg gaagagggca ctgcttataa atgctattgc
      301 tctaaagagc gcctggaagc gctgcgcgaa gagcaaatgg cgaaaggtga gaagccgcgt
      361 tatgacggtc gctgccgcca cagccatgag catcatgctg atgatgaacc gtgtgttgta
      421 cgttttgcta acccgcagga aggttctgtt gtttttgacg atcagatccg tggtccgatc
      481 gagttcagca accaggaact ggacgatctt attatccgcc gtaccgatgg ttccccaacc
      541 tataacttct gtgtggttgt cgatgactgg gatatggaaa tcacccacgt tatccgtggc
      601 gaagaccata tcaacaacac gccacgccag atcaacattc ttaaggccct gaaagcgccg
      661 gtgccggttt acgcgcacgt ttctatgatc aatggcgatg acggtaaaaa actgtccaaa
      721 cgtcacgggg cagtcagcgt aatgcagtat cgtgatgacg gttatttgcc agaagcactg
      781 ctgaactatc tggtgcgtct gggctggtcc cacggcgatc aggaaatctt cactcgtgaa
      841 gagatgatca aatacttcac tttgaatgcc gtcagcaaat ctgccagtgc gttcaacacc
      901 gacaagctgc tgtggctgaa ccatcactac attaacgcgc tgccgccgga gtatgttgct
      961 actcacttac agtggcacat tgagcaggaa aatatcgata cccgtaacgg cccgcagctg
     1021 gctgatctgg tgaaactgct gggcgaacgc tgcaagacgc tgaaagagat ggcacagagc
     1081 tgccgttatt tctacgaaga ttttgctgag ttcgatgccg acgccgcgaa aaaacatctg
     1141 cgtccggtag cgcgtcagcc gctggaagtg gttcgtgaca aactggccgc gattactgac
     1201 tggaccgctg aaaacgttca tcacgctatt caggcgacgg cggatgagct ggaagtgggt
     1261 atgggtaaag ttggtatgcc gctgcgtgtc gccgtaaccg gtgcggggca gtctccagca
     1321 ctggatgtta ccgttcacgc aattggtaag acccgcagta tcgagcgtat caacaaagcg
     1381 ctggatttta ttgctgaacg cgaaaatcag cagtaattag cgtgtaaaaa gataaacggc
     1441 aggagataat atcctgccgt tttttattta tgctgattcg tcaatatcga gacgggcgag
     1501 gaaattgcga attc
//