From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: anti-human erbB-2 receptor bifunctional single-chain
Date: 6 Jan 93 06:54:22 GMT
Distribution: bionet
Checksum: 45904 4

LOCUS       M245877S1     894 bp                    BCT       08-DEC-1992
DEFINITION  anti-human erbB-2 receptor bifunctional single-chain
            antibody-phosphatase fusion protein [mice, Other Synthetic
            Recombinant Partial, 894 nt, segment 1 of 2] 
ACCESSION   S46356
KEYWORDS    .
SEGMENT     1 of 2
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Unclassified.
REFERENCE   1  (bases 1 to 894)
  AUTHORS   Wels,W., Harwerth,I.M., Zwickl,M., Hardman,N., Groner,B. and
            Hynes,N.E.
  TITLE     Construction, bacterial expression and characterization of a
            bifunctional single-chain antibody-phosphatase fusion protein
            targeted to the human erbB-2 receptor.
  JOURNAL   Biotechnology (N.Y.) 10, 1128-1132 (1992)
  STANDARD  full automatic
COMMENT     This entry [NCBI gibbsq 115269] was created by the journal scanning
            component of NCBI/GenBank at the National Library of Medicine.
            This sequence comes from Fig. 1B and C.
FEATURES             Location/Qualifiers
     CDS             1..894
                     /partial
                     /note="Description: anti-human erbB-2 receptor
                     bifunctional single-chain antibody-phosphatase fusion
                     protein;  For the protein sequence (NCBI gibbsq 115272):
                     Method: conceptual translation supplied by author.  This
                     sequence comes from Fig. 1B and C.  The authors begin
                     their numbering at -21.  Zero is not present in the
                     numbering."
                     /codon_start=1
                     /translation="MKKTAIAIAVALAGFATVAQADYKDDDDKLASQVQLQQSGPELK
                     KPGETVKISCKASGYPFTNYGMNWVKQAPGQGLKWMGWINTSTGESTFADDFKGRFDF
                     SLETSANTAYLQINNLKSEDMATYFCARWEVYHGYVPYWGQGTTVTVSSGGGGSGGGG
                     SGGGGSDIQLTQSHKFLSTSVGDRVSITCKASQDVYNAVAWYQQKPGQSPKLLIYSAS
                     SRYTGVPSRFTGSGSGPDFTFTISSVQAEDLAVYFCQQHFRTPFTFGSGTKLEIKALE
                     PVLENRAAQGDITAPGGARRLT"
BASE COUNT      211 a    230 c    236 g    217 t
ORIGIN      
        1 atgaaaaaga cagctatcgc gattgcagtg gcactggctg gtttcgctac cgttgcgcaa
       61 gctgactaca aggacgacga tgacaagcta gcttctcagg tacaactgca gcagtctgga
      121 cctgaactga agaagcctgg agagacagtc aagatctcct gcaaggcctc tgggtatcct
      181 ttcacaaact atggaatgaa ctgggtgaag caggctccag gacagggttt aaagtggatg
      241 ggctggatta acacctccac tggagagtca acatttgctg atgacttcaa gggacggttt
      301 gacttctctt tggaaacctc tgccaacact gcctatttgc agatcaacaa cctcaaaagt
      361 gaagacatgg ctacatattt ctgtgcaaga tgggaggttt accacggcta cgttccttac
      421 tggggccaag ggaccacggt caccgtttcc tctggcggtg gcggttctgg tggcggtggc
      481 tccggcggtg gcggttctga catccagctg acccagtctc acaaattcct gtccacttca
      541 gtaggagaca gggtcagcat cacctgcaag gccagtcagg atgtgtataa tgctgttgcc
      601 tggtatcaac agaaaccagg acaatctcct aaacttctga tttactcggc atcctcccgg
      661 tacactggag tcccttctcg cttcactggc agtggctctg ggccggattt cactttcacc
      721 atcagcagtg tgcaggctga agacctggca gtttatttct gtcagcaaca ttttcgtact
      781 ccattcacgt tcggctcggg gacaaaattg gagatcaaag ctctagagcc tgttctggaa
      841 aaccgggctg ctcagggcga tattactgca cccggcggtg ctcgccgttt aacg
//