From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: aadA=streptomycin adenylyltransferase [Salmonella choleraesuis,
Date: 27 Jan 93 05:52:39 GMT
Distribution: bionet
Checksum: 06458 5

LOCUS       S116253      1920 bp    DNA             BCT       13-JAN-1993
DEFINITION  aadA=streptomycin adenylyltransferase  [Salmonella choleraesuis,
            Genomic, 1920 nt]
ACCESSION   S47520
KEYWORDS    .
SOURCE      Salmonella choleraesuis
  ORGANISM  Salmonella choleraesuis
            Unclassified.
REFERENCE   1  (bases 1 to 1920)
  AUTHORS   Leung,K.Y., Ruschkowski,S.R. and Finlay,B.B.
  TITLE     Isolation and characterization of the aadA
            aminoglycoside-resistance gene from Salmonella choleraesuis.
  JOURNAL   Mol. Microbiol. 6, 2453-2460 (1992)
  STANDARD  full automatic
COMMENT     This entry [NCBI gibbsq 116253] was created by the journal scanning
            component of NCBI/GenBank at the National Library of Medicine.
            This sequence comes from Fig. 2.
FEATURES             Location/Qualifiers
     CDS             265..1053
                     /note="3'(9)-O-nucleotidyltransferase;  AadA;  For the
                     protein sequence (NCBI gibbsq 116254):  Method: conceptual
                     translation supplied by author.  This sequence comes from
                     Fig. 2."
                     /gene="aadA"
                     /product="streptomycin adenylyltransferase"
                     /codon_start=1
                     /translation="MTLSIPPSIQCQTEAACRLITRVTGDTLRAIHLYGSAVAGGLKP
                     NSDIDLLVTICQPLTEAQRATLMQELLALSSPPGASAEKRALEVTVVLYSQLVPWCFP
                     PSREMQFGEWLREDICQGIYEPAQQDWDIVLLITQILETSIPLKGERAERLFTPAPVA
                     QLLKALRYPLDLWQSTADVQGDEYHIVLTLARIWYTLSTGRFTSKDAAADWLLPQLPE
                     EYAATLRAAQREYLGLEQQDWHILLPAVVRFVDFAKAHIPTQFT"
     CDS             1198..1758
                     /note="Description: orf 3' to aadA;  For the protein
                     sequence (NCBI gibbsq 116255):  Method: conceptual
                     translation supplied by author.  This sequence comes from
                     Fig. 2."
                     /codon_start=1
                     /translation="MTGSASSVLKTDTRLLSENLFLNYGLESLFKDVAIIAGNVNYII
                     FDIDDYYTVQQYITSTFKTVLIGIVTHNEYNFIDEHHAIYRIKVDASIAEWRELLILA
                     AAGQFFPRSEKVRLTANERNVLAMLVKGMDIRQISCELNVHLKTIYSVRYHVLTKLGC
                     RTVLDYQILSVSKAFHSKRPLKVWSG"
BASE COUNT      481 a    431 c    480 g    528 t
ORIGIN      
        1 gatcatatca ttgccccgcg caaatcggcg cacttccata tctttatggg aaatacctcg
       61 cagcaggcat tgttgcaaga gatggaaaac tggcctacct attatcctta tcagttaaag
      121 gcgaatgagg tcgttgacga aatgctacat cattaagtct gattgcaatg ttaaaagctc
      181 gctatgaata gcgggctttt tttctgccgt tatgccatat ttctggcaat caatataaaa
      241 aatacctgcg cccccggagc aacgatgacg ctgtccatac cgccctcgat tcaatgccaa
      301 acggaagcgg cgtgtcgcct gattactcgt gtcacaggcg atacgttgcg tgctattcat
      361 ttatatggtt ccgctgtcgc gggcggtctg aagccaaaca gcgacattga tctgctggtg
      421 acaatatgcc agccgctgac ggaggcccaa cgcgcaacgc tgatgcagga attgctggcg
      481 ctttcatcgc cgccgggggc gtcggcagaa aaacgcgctc tggaggtcac tgtcgtgctt
      541 tattcgcagc tggttccctg gtgttttcca ccttccaggg aaatgcaatt tggcgagtgg
      601 ctaagagagg acatttgtca ggggatttat gaaccagcgc agcaggactg ggacattgtg
      661 ttactgataa cccaaattct ggaaaccagt ataccgctaa agggcgaacg cgcggaacgg
      721 ctgttcacgc cagcccctgt ggcgcagttg ctgaaggcct tgcgttatcc cctcgacctt
      781 tggcaatcca cggcagatgt gcagggagat gagtatcata tcgttttaac cctggcgcgt
      841 atctggtata ccctttctac cgggagattt acctctaagg atgcggcggc tgactggctg
      901 ttacctcagt tgccagaaga gtatgctgct acgttgcgtg cggcgcagcg tgaatattta
      961 ggtctggagc aacaggactg gcatattttg ctgcctgcgg tcgtacgctt tgtggatttt
     1021 gcgaaagcgc acatccccac gcagttcaca taagatgccc caggacgtct gtcaggttgc
     1081 gcaaacggcg ttcctcaact actacttaat aggttctcat cgctgaagta agcagatgat
     1141 cttatgcggg ccatcgaatg gatattccca catggctctc gttttgttga ggtggatatg
     1201 actggttccg catcatctgt tttaaagaca gatacaagac tattgagcga aaacctgttt
     1261 cttaattatg gtctggagtc cttatttaag gacgtcgcaa taatagctgg caacgtaaat
     1321 tatattattt ttgatatcga cgattactac accgtacagc agtatattac ttccacattt
     1381 aaaacggtac tcattggaat tgttacccat aatgaatata actttattga tgaacatcat
     1441 gctatatatc gaataaaggt cgatgcctct attgctgagt ggcgtgagtt actgattttg
     1501 gccgctgctg gtcaattttt tcccaggtca gagaaagtca gattaacggc gaatgaaaga
     1561 aatgttttgg cgatgcttgt taaaggaatg gatatccgac aaataagctg tgagctaaat
     1621 gtacatttaa aaactattta ctcagtacgc taccatgttc tgactaaatt aggttgtaga
     1681 acagttctgg attatcaaat actgtccgtc tctaaagctt ttcattcgaa gagaccgttg
     1741 aaggtatgga gcggctaatc gcgcagggga aaattcgccg ttggggagtc tctaatcttg
     1801 attatgacga tatgcaggct ctgtggcgca tccccggggg acagcaatgc gcgacaaatc
     1861 aggtgcttta tcatctggcg tcacgcggta ttgagtatga tttattgccg tggtgtcaac
//