From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: S.cerevisiae nuf1 gene
Date: 10 Apr 92 10:15:00 GMT
Distribution: bionet
Checksum: 60090     7

LOCUS       YSCNUF1G     3522 bp ds-DNA             PLN       10-APR-1992
DEFINITION  S.cerevisiae nuf1 gene
ACCESSION   Z11582
KEYWORDS    nuf1 gene.
SOURCE      Saccharomyces cerevisiae DNA.
  ORGANISM  Saccharomyces cerevisiae
            Eukaryota; Fungi; Eumycota; Hemiascomycetes; Endomycetales;
            Saccharomycetaceae.
REFERENCE   1  (bases 1 to 3522)
  AUTHORS   Snyder,M.
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
REFERENCE   2  (bases 1 to 3522)
  AUTHORS   Mirzayan,C., Copeland,C.S. and Snyder,M.
  TITLE     The NUF1 gene encodes an essential coiled-coil related protein that
            is a potential component of the yeast nucleoskeleton
  JOURNAL   J. Cell Biol. 116, 1319-1332 (1992)
  STANDARD  full automatic
COMMENT     *source: strain=S288C; *source: clone_library=lambda gt11 and
            lambda EMBL3A; *source: is_macronuclear=N; *source: is_proviral=N;
            *source: is_germline=N;
            From EMBL    entry SCNUF1G;  dated 27-MAR-1992.
FEATURES             Location/Qualifiers
     CDS             232..3066
                     /gene="nuf1"
                     /translation="MDEASHLPNGSLKNMEFTPVGFIKSKRNTTQTQVVSPTKVPNAN
                     NGDENEGPVKKRQRRSIDDTIDSTRLFSEASQFDDSFPEIKANIPPSPRSGNVDKSRK
                     RNLIDDLKKDVPMSQPLKEQEVREHQMKKERFDRALESKLLGKRHITYANSDISNKEL
                     YINEIKSLKHEIKELRKEKNDTLNNYDTLEEETDDLKNRLQALEKELDAKNKIVNSRK
                     VDDHSGCIEEREQMERKLAELERKLKTVKDQVLELENNSDVQSLKLRSKEDELKNLMN
                     ELNELKSNAEEKDTQLEFKKNELRKRTNELNELKIKSDEMDLQLKQKQNESKRLKDEL
                     NELETKFSENGSQSSAKENELKMLKNKIAELEEEISTKNSQLIAKEGKLASLMAQLTQ
                     LESKLNQRDSQLGSREEELKKTNDKLQKDIRIAREETVSKDERIIDLQKKVKQLENDL
                     FVIKKTHSESKTITDNELESKDKLIKILENDLKVAQEKYSKMEKELKEREFNYKISES
                     KLEDEKTTLNEKISNLAAENSQLKNKIEDNSTATHHMKENYEKQLESLRKDIEEYKES
                     AKDSEDKIEELKIRIAENSAKVSEKRSKDIKQKDEQISDLTQNLKLQEDEISSLKSII
                     DRYKKDFNQLKSEQSNIQHDLNLQILNLENKLIESEDELKSLRDSQKIEIENWKRKYN
                     NLSLENDRLLTEKESASDKEREISILNRKLDEMDKEKWNLQESKEKYKRELQKVITAN
                     DRLRREKEELNENSNNIRIMEDKMTRIKKNYLSEITSLQEENRRLEERLILNERRKDN
                     DSTMQLNDIISYYKLKYHSEVRHNNDLKVINDYLNKVLALGTRRLRLDTRKGEHSLNI
                     SLPDDDELDRDYYNSHVYTRYHDYEYPLRFNLNRRGPYFERRLSFKTVALLVLACVRM
                     KRIAFYRRSDDNRLRILRDRIESSSGRISW"
     misc_feature    721..2605
                     /note="alpha helical (coiled-coil) coding region"
BASE COUNT     1426 a    528 c    682 g    886 t
ORIGIN
        1 ggagctcctt gtagacatta tttcatatgt tatacttaaa ctagatagag acgttttatt
       61 tgtactaagc tgaacattac cctaattacg cgttataagt tttaatcctt cattttgaag
      121 gaaaacaaat aaacaaaaag ttgatgtgga acaaatacaa tgtgcgcgga tatcttaagc
      181 aacttcgtta gttacagtcc acacaaagcg aaaaagggct gataaacact catggacgaa
      241 gcgtcacatc tcccaaatgg gagcttgaag aacatggaat ttacgcctgt aggatttatc
      301 aaatccaagc gaaacaccac gcaaacacaa gttgtatcgc ctactaaggt tccaaatgcc
      361 aataatggtg atgagaacga aggccctgtt aagaaaaggc agagaagaag cattgatgat
      421 acaattgact ccacaaggct atttagtgaa gcttcacaat tcgatgacag ctttccagaa
      481 attaaggcta acattccgcc tagtccaagg tcaggcaatg ttgacaaaag tcgcaagaga
      541 aatttgattg atgatttgaa gaaagatgtg ccaatgtctc agcccttgaa agaacaagaa
      601 gtaagagaac accaaatgaa gaaagagcga tttgaccgtg ctttagagag taaattacta
      661 ggaaaaagac acataacata cgcaaattct gatatttcta ataaggaact ttacattaat
      721 gaaatcaaga gtttgaagca tgaaatcaaa gaattaagaa aggaaaaaaa cgatactctc
      781 aataattatg atacccttga agaagaaaca gatgacttga agaacagatt acaagcgctg
      841 gaaaaagagc tggacgccaa aaataaaatt gtgaattcaa gaaaagtaga tgatcattct
      901 ggatgcatag aagaacgtga acaaatggaa agaaagttgg ctgaattaga aagaaaactg
      961 aaaactgtga aagaccaagt gctagaatta gagaataata gtgacgtaca aagtttaaaa
     1021 ttgagatcta aggaggatga attgaagaat ttaatgaatg agttgaatga attgaagagc
     1081 aatgcagaag aaaaggatac acagttggaa ttcaagaaaa atgaactgag gaaacgaaca
     1141 aatgaattaa atgagttgaa aatcaagtct gatgagatgg atttacaact aaaacaaaaa
     1201 caaaatgaat caaaaagatt aaaagatgaa ttaaatgagc ttgaaaccaa attcagcgaa
     1261 aatggttctc agtcttctgc aaaagaaaat gaattgaaaa tgctgaaaaa taaaatagcc
     1321 gagctagagg aagagattag cacgaaaaat tcacagttaa tcgcaaaaga aggtaagtta
     1381 gcatcattaa tggctcagct aactcaattg gagagtaaac ttaatcaaag agactcccag
     1441 ttgggctcaa gggaagaaga attgaaaaaa acaaacgata agctacaaaa agatatcagg
     1501 atagcaagag aggaaacagt ttcaaaggat gaacggataa ttgatcttca aaaaaaggtt
     1561 aaacagctag aaaatgactt atttgtgata aaaaaaacgc acagtgagtc taaaactatt
     1621 actgataatg aactagaatc taaagataaa cttattaaaa ttttagaaaa cgatttaaag
     1681 gttgcacaag agaagtactc taaaatggaa aaagagctca aagaaaggga atttaactat
     1741 aaaatttccg aatcaaagtt ggaagatgaa aagaccacgc taaatgaaaa aatttctaac
     1801 ttagccgcag aaaactcaca gctaaaaaat aaaatagagg acaattcgac tgccactcac
     1861 catatgaaag aaaactatga gaagcagtta gaatcgctaa ggaaagatat tgaagagtac
     1921 aaagaaagcg caaaagattc tgaagacaaa attgaggaac taaaaattag gattgctgaa
     1981 aattctgcta aagtatcgga gaaaagatca aaggatataa aacaaaaaga tgaacagatc
     2041 agcgacctca ctcagaatct aaaactacaa gaagatgaga taagctcatt aaaatccata
     2101 attgacaggt acaaaaaaga tttcaatcaa ttgaaatctg aacagagtaa tatccaacat
     2161 gacctaaatt tacaaatact aaatctggaa aataagttaa tagagagcga ggatgaatta
     2221 aagtcactaa gagattctca aaaaattgaa atagaaaact ggaagagaaa gtataacaat
     2281 ctttcactgg aaaatgacag attgttgaca gaaaaagaat ccgcatcaga caaagagcgc
     2341 gagatatcca tcttgaacag aaaacttgat gaaatggata aagaaaaatg gaacttacag
     2401 gaatctaaag aaaaatataa acgcgaacta cagaaggtga ttactgctaa tgatcgtttg
     2461 agaagagaga aagaggagtt gaatgaaaac agcaataata ttcgtatcat ggaagacaag
     2521 atgactagga taaaaaaaaa ttatttgagt gaaatcactt ctttacaaga ggaaaatagg
     2581 agacttgaag aacgcctcat attaaatgag aggcgtaaag acaatgattc aactatgcaa
     2641 ttaaatgaca tcataagcta ttataaattg aagtatcact cagaagtaag acacaacaac
     2701 gatcttaagg tgatcaatga ttatttaaac aaggttcttg ctttggggac ccgtcgttta
     2761 agattggaca caaggaaagg tgagcacagt ctaaacattt cacttccaga cgatgatgaa
     2821 ctagatcgtg attactacaa tagccatgtt tatacaaggt atcatgacta tgaatacccg
     2881 cttagattca atttaaacag aagaggtccc tatttcgaac gccgcttaag cttcaaaaca
     2941 gttgcccttt tagtgcttgc ttgtgtgagg atgaaaagga ttgcttttta caggagatca
     3001 gacgataaca gattgcgaat actaagagat agaattgaga gtagcagcgg gcgtatatct
     3061 tggtagtatc gcttactcta tcatcatatt tctcgtatgt acatcgactc ctacgcagac
     3121 tgatcttcaa gcgagccacc gtgaatgtgg aagcataaac agatgagaaa cctttttgtt
     3181 tctgagatcg tgaccttaga ttgtagtgtg ctaatctccc tagtattgac tactttgcgt
     3241 ttattcattt ctttctccaa tagaaataca gcataaaaca tacgtagggt atatctatgt
     3301 atgtggtata tttgtattat aagttactta gatttactgg cgcccttttt taagctattg
     3361 taattattta cggcctctgc gattctttcc ttgaaagtat ctgttattat cgccagttgg
     3421 tttaacattt catagtgatt ttccaacgta tcgcattttt caaggagttc tttgaacttc
     3481 gcaatatttt tatctatggt gttttcaata ataccattca cc
//