From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: N. sylvestris DNA for chloroplast 33 kd ribonucleoprotein, complete
Date: 12 Jan 93 05:51:12 GMT
Distribution: bionet
Checksum: 11854 9

LOCUS       TOBGRNP      4832 bp ds-DNA             PLN       12-JAN-1993
DEFINITION  N. sylvestris DNA for chloroplast 33 kd ribonucleoprotein, complete
            cds.
ACCESSION   D11111 X58356
KEYWORDS    RNA binding protein; chloroplast 33 kd ribonucleoprotein; cp33;
            ssDNA binding protein.
SOURCE      Nicotiana sylvestris leaf DNA.
  ORGANISM  Nicotiana sylvestris
            Eukaryota; Plantae; Embryobionta; Magnoliophyta; Magnoliopsida;
            Asteridae; Solanales; Solanaceae.
REFERENCE   1  (bases 1 to 4832)
  AUTHORS   Sugiura,M.
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
REFERENCE   2  (sites)
  AUTHORS   Li,Y., Nagayoshi,S., Sugita,M. and Sugiura,M.
  TITLE     Structure and expression of the tobacco nuclear gene encoding the
            33 kd chloroplast ribonucleoprotein: the existence of a TATA-less
            promoter and a partially spliced mRNA
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
COMMENT     Submitted (13-May-1992) to DDBJ by: Masahiro Sugiura Center for
            Gene Research Nagoya University Chikusa-ku, Nagoya 464-01 Japan
            Phone:  052-781-5111 x3449 Fax:    052-782-3849.
FEATURES             Location/Qualifiers
     exon            <845..1261
                     /number=1
     mat_peptide     join(1043..1261,2147..2248,3436..3744,3823..3951)
                     /product="cp33 protein"
                     /codon_start=1
     misc_feature    664..820
                     /note="multiple transcriptional start sites"
     transit_peptide 845..1042
     intron          1262..2146
                     /number=1
     exon            2147..2248
                     /number=2
     intron          2249..3435
                     /number=2
     exon            3436..3744
                     /number=3
     intron          3745..3822
                     /number=3
     exon            3823..>3954
                     /number=4
     CDS             join(845..1261,2147..2248,3436..3744,3823..3954)
                     /product="chloroplast 33 kd ribonucleoprotein (cp33)"
                     /codon_start=1
                     /translation="MSVAATASTCSTSSLYLFTQKPKFSVEHLSLSTYNAHFNFKINS
                     TKLKAHFPISSLYRSSIFLSTCASVSDGVEVVQEDDEEEVALSAEEEEEIEEKEESVE
                     SESVEGGRLYVGNLPFSMTSSQLSEIFAEAGTVANVEIVYDRVTDRSRGFAFVTMGSV
                     EEAKEAIRLFDGSQVGGRTVKVNFPEVPRGGEREVMSAKIRSTYQGFVDSPHKLYVAN
                     LSWALTSQGLRDAFADQPGFMSAKVIYDRSSGRSRGFGFITFSSAEAMKSALDTMNEV
                     ELEGRPLRLNVAGQKAPLSSPSVVETSPENDSENNELLSSLSS"
BASE COUNT     1462 a    843 c    885 g   1642 t
ORIGIN      
        1 gaattcgtct ctataattcg ccaacttgtt ttctttaatc tcttaattta ctactctaga
       61 ttaattttag ttaaatattc atacgttagg attcgcttga atagattaat tgtttggttt
      121 aatttagttg atagttaatc acaagtccct gtgggtacga tatctagact tacaatctta
      181 tattacttgt cgatcactta tacttgcgtg tgcgtttggg agcaacagaa acgcaattat
      241 tgttctctaa taatgtttca acgcacaaca actgaaaaga aataatagca atgcacgata
      301 ctattagtag cagcaatatt caacctaaac actgaattta aaaattaaaa aaaaaaaagc
      361 ttccgctgtg ccgcaggatc cggggaaagg ccggaccaca agggtctatt gtacctagcc
      421 ttaccttgca tttctgcaag aagttatttc cacggctcga acccgtgacc tcctggtcat
      481 atgacagtaa ctttagcagt caaggctccc cttgaaattt ttttaaaaaa aatagaaaat
      541 ggaaataatg atattaatgg gtgcataatt ttgtggggag agttttttgt ttgccttact
      601 attatcaaag ttggagagtg attttctaaa atatactatc tggacttcaa aaatctaaat
      661 agacatgagt gtcactcatt caaataagca cttgaagaaa tgaaaaggcg gcgtaataga
      721 gaggacgaca aatccaaaat taattcctga aaaccccata tctcggtctt atccgccaaa
      781 cccattaaaa ccaacctcaa attttatcct ctcgtttcca cccgaactgg ttctttggta
      841 caaaatgtca gttgccgcta cagcatctac ttgttctact tcttcactgt atctcttcac
      901 tcaaaagcct aaattctcag tagaacatct ttccttaagt acttataatg ctcacttcaa
      961 cttcaagata aactcaacaa aactcaaagc ccatttcccc atatcttctc tttatcgctc
     1021 ttcaatcttt ttgagcactt gtgcttctgt gtctgatgga gttgaggttg tccaagaaga
     1081 tgacgaagaa gaagttgcat tgtcagccga agaagaagag gaaattgaag aaaaagaaga
     1141 aagtgttgag tcagaatcag ttgaaggtgg tagattgtac gtgggtaatt tgcctttttc
     1201 aatgacctct tctcaattgt ctgaaatctt tgctgaagct ggcacagtgg ctaatgttga
     1261 ggtctgtttt gagtttttgt tgctttgatt ggattctttt taattttctt ctttggctct
     1321 tttctgttca tgtactgaag ttccaattat tttactgatt aggcaacgca atcctattac
     1381 tatgaactct aacattgatc attgagattg gtaattaaga ctaattgtca gttgagcagg
     1441 tgcctctgct aagatgcaaa atatctgtca ttgttgaaaa atgatttacc ttaaagaact
     1501 attatatagg tcagtttttg gggtatggtt ggacataaag ataggtggca tcaaagtcgg
     1561 atccaagatt tgaactttat ggatttgcat tcgggattct accacaactt atatgattta
     1621 catggttcga gatcaattat ttgtactaaa gaaaattttt caacacatat acaagatcca
     1681 agccacaatc taggttcgga tgaactcatg cctagaatgc taagtggaat atggtagtca
     1741 tagaagttgt aaatgttgat tgctcttgtc tgctatttat caacttttta ctaattatgt
     1801 aacaaaatct catagcattg acattctgaa ttgatttttg agacgggcaa gactaatgta
     1861 gatgacgtaa ttcaaatcct taaatcattt gagataaagt agagggtttt tttttttatt
     1921 ttttttatct tgatgccaga gaaaattggt ttgttattgt ggattataac tgaaagaaga
     1981 cttttcgcat tgagtagatt aacgtagctg tagttgaaac tgggctcatg atttccaagt
     2041 atcaatcttg tttgtacttt agttcgacat ctgatggatg tcatattctt ttttagaata
     2101 tatgataaag tgaaactcat tgttcttttt cttcatggat ttatagattg tttatgacag
     2161 agttacagat aggagtcgtg gatttgcatt tgttacgatg ggaagtgttg aagaggcaaa
     2221 ggaagcaatt cgcttgtttg atggatctgt aagtttacac tccgctcatc attttattgc
     2281 aaggactctt ttatgcattg ctaagctgtc aaaaagaact tatttgtctg ggatgccaaa
     2341 aaatagtaat acccctgtta aattttgata gtcaatcatt aggaatataa ttaagatggt
     2401 gtgttattcg cctatcttga aaactttgta aaagggtagc ccagtatcca aaacatcctg
     2461 cattcacgca aggtccgggg aagggccgca agccaagggg tgtaatgtag ataatctacc
     2521 gtaatgcaag cattaatcac acggagacaa cttaaccgtt gctccaaggc tccccttgaa
     2581 ctttgtctcg ataagaaaac atgttttgtc tccttgtcat tcattgctca tctgaatgat
     2641 ggatgttttt acctatgcat ctattaaaaa atgtagttaa ttattttcct actacttcaa
     2701 tttatggtaa aggcatatca gtgttctaaa tatgtaacaa ctgctagata gatttcttct
     2761 ctgtgtaata tgtagcttgg gtcctctttg tttcttggta tctgtaactt ctataaggta
     2821 tccttttttt ccctctttct ggggagaaaa gaggaataaa ctagtaatca gtcaagtttg
     2881 acaaagctag taatcgggct gtgtaagaca tttttactac agttttacag aatgcactct
     2941 gaagcttact aaacgctctg aaatatggca tagagagcaa ctgatgttca cagctcgaaa
     3001 atcatccaca aaatagatta tagccattac ttgtcatctg gccgtatcag tttcagaggg
     3061 ttattgcact cgttgatgca cgaattctca catgctctcc ttggtgccta gagaaaaggt
     3121 aaattgagga cacaactgtt cacatgatat gggaaacaaa caggaatcgt atgaattctt
     3181 tgcaaagacc aaaagtccaa tgtttacctg ctagatgatt atcaaatgag aaagatgtga
     3241 catgtctcta cttaataaag tcagtatatc aataatcaat atggaagcca ttacaaaagt
     3301 ttgaagtaat ctgtatttgt cctttgtgac cagtttgttt gtcgtattga tttagtcgga
     3361 gaactgtcca atgtactctt ttcttacatt gcccctcttt tctctttcct tgctatttgt
     3421 ggtgaatctt aacagcaagt tggaggtcgt acggtcaagg tgaatttccc tgaggttcca
     3481 agaggaggtg aaagggaagt aatgagtgca aagataagaa gcacctatca aggttttgta
     3541 gatagccctc acaaattata cgttgcaaat cttagctggg ccctcacttc tcaaggtcta
     3601 agagatgctt ttgctgacca gcccggattc atgagtgcaa aagtcatcta tgacaggtcc
     3661 tctggaagat ctcgaggttt tgggttcatt acattttctt ctgctgaagc aatgaaatct
     3721 gcacttgata ccatgaatga agtggtaagg atacatagtg atgaagtatc ttggagacct
     3781 tttggcatat ccttgtttct cacagtttta taatgcaatt aggaacttga aggacggccg
     3841 ttgcgactaa atgtggctgg gcagaaagct cccctatctt ctccatcagt tgtcgaaaca
     3901 agtcctgaaa atgattctga gaacaatgaa ttactttcta gtctcagctc ataagatcac
     3961 caagattcat aacagaatca gccatttgga gtaatgtaga gttttatatg cggtgaagac
     4021 atgctttttc cagcttatgt aaccttctcg cagcctcggc ttctttttgc accagttatt
     4081 gtctgctatt ggtctgtcac ttgatgcagg taagctgtgt tgctacattg cctgtgcatt
     4141 ttcaaaatgc acagttacca aatgtatttt gattgtaata taactcatcg cttgaatcaa
     4201 tagagatact catgcaaaat gctcaatgct catatattca gctactttat atttccttat
     4261 atcatactca ttctatattt taaaatttta tttctgattg tctgcagtaa attataattg
     4321 taaatcaaaa ttttacacat atttctgctc aattaactat agggtttctt gcttccttat
     4381 tatatgatga acaatattat ctttctcatt ttaattagta tgtatctgtt gggaaaaaaa
     4441 ttctaatgtt ttttcggggg ctctcctaac gcttctacgt aagtggttat tttttaggaa
     4501 atagtaaatg taagttggaa ctaagggtgt gtttggtaca aaggaaaaca ttttccagaa
     4561 aatgtttttc aattttccta tgtttagttg gcttaatagt ttggaaaaca ttttcttcat
     4621 gaacttattt tcctccaatt ggaggaaaat atttgcccta tcaagaaaag ggaaaatatt
     4681 tttctaaaaa aaaatctact ctctaaccaa acactaggaa atatttttcg gaaaaagttt
     4741 tccactcatc aatcaaacaa ggaaaaatta gtcataaaat cactcatttt ctaggaaaac
     4801 attttccttc ctaccaaaca caccctaagc tt
//