From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Thermus thermophilus endonuclease and methylase genes, complete
Date: 9 Apr 92 10:05:31 GMT
Distribution: bionet
Checksum: 56137     5

LOCUS       TTHEA2AM     2188 bp ds-DNA             BCT       09-APR-1992
DEFINITION  Thermus thermophilus endonuclease and methylase genes, complete
            cds.
ACCESSION   M74795
KEYWORDS    endonuclease; methylase.
SOURCE      Thermus thermophilus (strain HB8) DNA.
  ORGANISM  Thermus thermophilus
            Prokaryota; Bacteria; Gracilicutes; Scotobacteria; Neisseriaceae.
REFERENCE   1  (bases 1 to 2188)
  AUTHORS   Brany,F., Danzitz,M., Zebala,J. and Mayer,A.
  TITLE     cloning and sequencing of genes encoding the TthHB8I restriction
            and modification enzymes: comparison with the isoschizomeric TaqI
            enzymes
  JOURNAL   Gene 112, 3-12 (1992)
  STANDARD  unannotated staff_entry
FEATURES             Location/Qualifiers
     CDS             102..1388
                     /product="methylase"
                     /gene="methylase"
                     /codon_start=1
                     /translation="MLFPPSLPPTASGRSLGRVETPPGLVRFMVGLAEARKGVRVLEP
                     ACADGPFLRAFPEAHGTGYRFVGVEIDPHALDLPPWAEGVVADFLLWEPGEAFDLILG
                     NPPYGIVGEASKYPIHVLREVKGLYKKTLSTWKGKYNLYGAFIEKSVRLLREGGTLVF
                     VVPATWLVLDDFSLLRSFLAREGRTEVYYLGEVFPGRKVSAVVLRFRKGGKGLALWDT
                     RRDGETFTPLLWSEKPGMEGRDHRFETGWTREMEASGPPLGSLFHIPFAARSPEFKKH
                     PAVQKEPEPGLVPVLTGRNLKPGWIDYESNHSGLWMPKERAKELRDFYATPHLVVAHT
                     KGTKVVAAWDEKAYPWREEFHLLPKEGVELDPLFLVEWLNSDKIQEYVKTLYRDFVPH
                     LTLRMLERIPALLPRKGNTERRKHGPYTSPESAGSF"
     CDS             1348..2139
                     /product="endonuclease"
                     /gene="endonuclease"
                     /codon_start=1
                     /translation="MAPTQAQKVLEAFEDFLKCLDLESYQEKYRPIKTVEQDLPRELN
                     PLPDLYDHYWKPNGNTLHFPDFETFFDQWWEKRLRPLNEFIRKYFWGCSYEFVRLGLE
                     ARLYRTAVSIWTQFHFSYRWNASCQLRLTATWELDAQGIDAQIQAEDRLIGIQIKKET
                     YRSEAREGNRFLRRREHSALLEVPYTLQSPEELERKAQRARTREEAYRLWVKIAHHLE
                     RLPNGFVIFRESYVKDLENFLKQNATTLSGLIPWDKVAREALTGP"
BASE COUNT      523 a    627 c    627 g    411 t
ORIGIN
        1 ggatccgcaa catcgtcacc gccgcccaca cgaaggagat gctggacaag ccctcgaggc
       61 ctacgagaag gtgggcaaga ggctcggtat aatccgctaa gatgctcttt cctccttccc
      121 taccccctac ggcctccggg cgaagcctgg gccgagtaga aaccccacca gggctagtgc
      181 gcttcatggt ggggctcgcc gaggcccgaa agggggtaag ggtcctggaa cccgcctgcg
      241 cggacgggcc cttcctccgg gccttcccgg aggcccacgg gacgggctac cgcttcgtgg
      301 gggtggagat cgacccacac gccctggacc ttcccccttg ggcggaaggg gtggtggcgg
      361 acttcctcct ctgggagccc ggggaagcct ttgacctcat cttgggcaac ccgccctacg
      421 gcatcgtggg ggaggccagc aagtacccca tccacgtcct tagggaggtc aaggggcttt
      481 acaagaagac cctatccacc tggaagggca agtacaacct ttacggggcc ttcatagaga
      541 aatctgtgcg cctcttgagg gagggcggaa ccctggtctt cgtagtcccg gccacgtggc
      601 tcgtcctgga cgacttttcc ctccttcggt catttctggc ccgagaaggg agaacagaag
      661 tttactatct aggcgaggtc ttccccggaa gaaaggtgag cgctgtggtt cttcggttcc
      721 ggaagggagg aaaaggcctc gccctttggg acacccggag agacggggag accttcacgc
      781 ccctcttgtg gagcgagaag ccgggaatgg aagggagaga tcatcgcttc gaaacagggt
      841 ggaccaggga gatggaggcc tcgggtccac ccctaggtag cctattccac atcccgttcg
      901 ccgccaggag cccggagttc aagaagcatc ccgccgtcca aaaggaaccg gaacctggcc
      961 ttgtgcccgt cctgacggga aggaacctga agccggggtg gatagactac gaaagcaacc
     1021 actccggcct ttggatgccc aaggaaaggg ccaaggagct tagggacttc tacgccacgc
     1081 cccacttggt ggtggcccac actaagggca ccaaggtggt agccgcttgg gacgagaagg
     1141 cctacccgtg gagagaagag ttccaccttc tgcccaagga gggtgtggaa ctagacccct
     1201 tgtttctggt agagtggtta aactccgata agatacaaga gtatgtcaaa accctttaca
     1261 gggactttgt tcctcacttg acgcttagaa tgttggagcg gatacctgcg ttgcttcccc
     1321 gaaaggggaa caccgagagg aggaaacatg gcccctacac aagcccagaa agtgctggaa
     1381 gcttttgagg attttctgaa gtgtttggac ctcgagagct accaagaaaa ataccgcccc
     1441 atcaaaacag tagagcaaga cctacctaga gagcttaacc cacttccaga cttgtacgac
     1501 cactattgga agcccaacgg gaacaccctt cactttccag attttgaaac tttcttcgac
     1561 cagtggtggg agaagcgcct ccggccccta aacgagttta ttcgcaagta tttttgggga
     1621 tgttcctatg aatttgtccg tctcggctta gaagcgaggc tctaccggac cgctgtttcc
     1681 atttggacgc aatttcactt ttcgtatcgc tggaacgcct cttgtcaact tcgcttgacg
     1741 gccacctggg agttggacgc ccaggggata gatgcacaaa ttcaagcaga agaccgcctg
     1801 ataggcattc agataaaaaa ggaaacctat cgctcggaag cccgggaggg aaaccgcttc
     1861 ctaagaaggc gcgaacattc cgccctcctg gaagttccct acacgctgca aagcccagag
     1921 gaactcgaaa gaaaagccca gcgtgcccga accagagaag aagcctaccg cttgtgggtc
     1981 aaaatcgccc accatctaga acggcttccc aacggattcg tcatcttccg agaaagctac
     2041 gtaaaggact tggaaaactt tttaaagcaa aacgccacta cattgtccgg actcataccc
     2101 tgggataagg tagcccggga agccctcacc ggcccgtgag gtacacccgg aggacgagcc
     2161 cagagaagag aagccccagg gccacgaa
//