From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Hordeum vulgare 1,3-1,4-beta-D glucan 4-glucanohydrolase (Glb 2)
Date: 28 Aug 92 10:00:24 GMT
Distribution: bionet
Checksum: 14420     8

LOCUS       BLYGLB2      5159 bp ds-DNA             PLN       28-AUG-1992
DEFINITION  Hordeum vulgare 1,3-1,4-beta-D glucan 4-glucanohydrolase (Glb 2)
            gene, intron 1.
ACCESSION   M62740
KEYWORDS    1,3-1,4-beta-D-glucan 4-glucanohydrolase; lichenase.
SOURCE      Hordeum vulgare (strain Northrup King 1558) DNA.
  ORGANISM  Hordeum vulgare
            Eukaryota; Plantae; Embryobionta; Magnoliophyta; Liliopsida;
            Commelinidae; Cyperales; Poaceae.
REFERENCE   1  (bases 1 to 5159)
  AUTHORS   Wolf,N.
  TITLE     Complete nucleotide sequence of a hordeum vulgare gene encoding
            (1-3,1-4)-beta-glucanase isoenzyme II
  JOURNAL   Plant Physiol. 96, 1382-1384 (1991)
  STANDARD  simple staff_entry
REFERENCE   2  (sites)
  AUTHORS   Wolf,N.
  TITLE     Structure of the genes encoding Hordeum vulgare (L3,
            L4)-beta-glucanase isoenzymes I and II and functional analysis of
            their promoters in barley aleurone protoplasts
  JOURNAL   Mol. Gen. Genet. 234, 33-42 (1992)
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     TATA_signal     941..947
                     /gene="Glb 2"
     intron          1109..4060
                     /number=1
                     /gene="Glb 2"
BASE COUNT     1501 a   1185 c   1061 g   1412 t
ORIGIN
        1 ttacttgtta acttattata aattatcttg gtatcaaact atcttacaac acttgccatg
       61 aaaccttact aaaacgcaat tccttccgct ccttgccggg ttcgacactc ttaattgtca
      121 aaaagactgt gattgatccc ctatactcgt gggtcatcaa caagccgtcg tctcctcttg
      181 agaatcctca cctttcatgc ggcatcatga tttttcaaat cgatctatca aagcttgctt
      241 gtcggccttt gtaagtgcct ccaccgagag tgggggttga tacgtctcaa acgcattgat
      301 aattttagta caattcctgc tatgttcgta tcaattataa acattttggg catactttta
      361 ttgagtttta tgataaacta aattgttgat ttcttgcaaa atggtcacaa aaagaattat
      421 atgtagaaat aagagtaaac atagatccta gcatgaggca gtaaaaggta gagtgacgaa
      481 aggcatctag tgtacaccga aatattttaa attgaccacg gatattcata agtaaactac
      541 gtgagtcatg tcggataccg tagaagacgg tctttctatg aaaaaccgtc accaaatgtc
      601 gttgagtaag acgtgaacac atccggacat ccggtgtcac gcaaaccacc atttgcctaa
      661 aaacttgttt ctcatggtac attcgtggag tccaaacttc aaactatttt tctcaaaagg
      721 ttgtttggtg ctactatgaa tgaattagcc caccacctct cctacttgca ataacaacct
      781 ggataacctc tgcccaccag acactacgag aagggggtga cgcatgcatg caaacggcct
      841 aggtagccta tcacaacagg ctaaatacga aataaatgtc cctggagacg ttgggcttgc
      901 aagtggattg gaccaaatta aactctgtct cctaggagcc tatataaggg gcatggcctc
      961 accatagaag aaagacgaga gtcttacttg ccccctccaa cgcaggtaga gagaaagaga
     1021 gagtttcgag acccaatggc gagccaaggc gttgcctcca tgttcactct cgcattgctt
     1081 ctcggagcct tcgcgtctat cccaccaagt gattcccctt ctccctttct ctctcgcccc
     1141 aaatgattgc ttacagagtt ttttttccat gcaagtcatg catatagctt ttttggaggt
     1201 acccctcctt ttctaaatat aaggtttttt agagatttca ttaggagact acatacgaag
     1261 caaaatgatg aattataatt taaagtgtgt ctatatacat ccgtatgtac tcttttagtt
     1321 taaaaatact tatatttagt aatgaaaaaa gtagaagata ataaattctt tgtttcgaaa
     1381 tataagacct tctatagatt ccactaaaaa ctatatacgg atcgacatat atagacacta
     1441 ttagagtatg cgtgcgttta ttttatttta tatatagttc ataataaaat ctctaaaaaa
     1501 tcttaatttt agaaacggag ggagtaatac gtacactaat cttagatttt attaagagaa
     1561 gcattatagc tagataggta cgttaatcct aggcatacat taatcagggg tacatcttgt
     1621 accggccaca cctagatgat agtgcacgtg taatcgacct cagtgtgaaa aagataacgg
     1681 cgcagaagta ggtattccgc agggaaatat aaatcttaaa aataatgaaa atgacatttt
     1741 ctagataaaa cgttggaaga ggtataaaaa gataacggcg caagaagtag gttttccacg
     1801 agggagtaat caatgaaaac gatacttgta gatactttca actgtttaac tatagaaagt
     1861 taatctagtt aatctttctt ttgaaacagg ctttcgtccc gttttacata taaaacaaca
     1921 acaccaagtt aaacaccaga tcgatacaat aaatccctaa ataactggcc aacataaccg
     1981 catgcggcta cgttgctgaa caacaaatca caggatgaca atattacaac gccatgacaa
     2041 gagttaggac atctagcctt gacaacaacg ccctcaaaag gcaaacacaa gcaccgccgc
     2101 tgccgaccta ggacaactag ttgatcgttc aagcaaaagc ataatataaa agtttgcaaa
     2161 tgaaatctgg aaatgtttga tgagcatgcc agttagccat cttcttatta agtcagaagg
     2221 ttgaactaac gatctataaa ggaaaacata gctaagccca gtgtcatgtt aattacgtag
     2281 aaagtttttc ttcatccctg catgcatggc ttgcgcacaa acacacacta ggcaggagtg
     2341 tcgccgtagc actgttggca cgagcctagc tagctactac cccggtactg ccactgttcc
     2401 attcttttaa gtttaaagaa aatgattgca ccagctagta actgactcat ttgcagtctt
     2461 gtgtagttat cataacttca aagagcacca acatatcctt tctgaagatt gtggactgta
     2521 cttatgaatc ttatctattc ataaacagag aagattcatc gtgatgatcc atatccctta
     2581 caagcgctga gtactaatgg tcttgctttt aaaaagtttc atcaaatagt taatgatata
     2641 caacaccgta tatatttaga aaaaaatcta agacaattaa tttgggacaa agaaagtact
     2701 attattcact tcgtttttca tatatacttg ctcatcatac tgtagctact ttctatataa
     2761 cttaatacta gacatttttt tactaatgtt cctcggcctt tgaacacgtg aaaaatacca
     2821 tctaacacca acttgtgact gtaaaaaaca ctattttagt cgggcatcaa ccactagagt
     2881 ctagagggtg aaagagataa aaaaatgtta tgcaaacctg aagctccatc catgcacgca
     2941 tccacgcatg cctataggat tttagttaaa cgtcctttaa tttcggtgag atccctcgcc
     3001 aacagctcaa gcctgccgct cgcttgttgt gtgttcatga atcgccgtca ttatattttg
     3061 gcttagggca tctcgctgac cctcaaagaa ttctgagttt ttttagaaaa ttcagaatat
     3121 tcgatttttc atattttcaa cacagatata catgaaggca taaaacacac gtgtataaat
     3181 ttttaagaca aaatacatag aaatgaggag tatgtgcgaa aaacataaat ataaggcttt
     3241 ttaacacatg atactattca tccttccaga ccatgaagtt gtttttttat acagatcgca
     3301 tttcaatttt ttttatcctg aaattttgca cacatacaca taacattctt gttaatttac
     3361 acaattgttt ttagatattt ttggaaccga aaagtttgag tttttttttc tgttcaagaa
     3421 tttatccagt taaccagtta acttgccgat taatgcctac tcgtagggcc attgagtagc
     3481 cgattaactg ataatttgcc taattaatga attaaatgga caattaacat gccgattagc
     3541 tcattaatct cctactaacc agccaaccga aaagttacca gttaacgatt tcctcaataa
     3601 ttttttgtaa gaaaggcctc cacggatacc tagagccagc caaacctcag ttcgtgtgtg
     3661 tgtggggggg gggggtatgg ggtggctatg tggcagcttt ggtgtaaatg tccaaactgg
     3721 ctatgtggca gctttggtgt aaatgtccaa actaccgcaa agcttctttt cgtttagttt
     3781 tgatttgcgg aataacgaac gtccggacca aaccacgaac gaatgccgga ctgcattaga
     3841 cgcttgaagg agatttgaga gtccacttta aaaatggcct taatgtttct ttccatttct
     3901 tttttattcc gtcacatgtc atcacgggag gaatttccta tggcaatgga aggaacagta
     3961 ctgctactac ctggtgaatt ttttttaaaa aattgcatgt ggatagtaca aggttcacta
     4021 accaaagcta catgcatgga tgaaccttac gcgcatgcag gcgtggagtc gatcggggtg
     4081 tgctacggca tgagcgccaa caacctgccg gcggcgagca ccgtcgtgag catgttcaag
     4141 tccaacggga tcaaatcgat gcggctgtac gctcccaacc aggcggcgct gcaggccgtc
     4201 ggcggcacgg gcatcaacgt cgtcgtcggg gctcctaacg acgtcctctc caacctcgcc
     4261 gccagcccgg cagcggccgc ctcgtgggtc aagagcaaca tccaggcgta ccccaaggtt
     4321 tccttccggt acgtctgcgt cggaaacgag gtcgccggcg gcgccacccg gaacctcgtc
     4381 ccggcaatga agaacgtgca tggcgcgctc gtcgccgctg ggctgggcca catcaaggtg
     4441 accacgtcgg tgtcgcaggc catcctcggc gtgttcagcc cgccctccgc cgggtccttc
     4501 accggggagg cggccgcgtt catgggcccc gtggtgcagt tccttgcccg caccaacgcg
     4561 ccgctcatgg ccaacattta cccgtacctg gcctgggctt acaacccgag cgccatggac
     4621 atgggctacg ctctcttcaa cgcgtccggc accgtggtca gggacggcgc ctacgggtac
     4681 cagaacctgt tcgacaccac cgtggacgcc ttctacacgg ccatgggcaa gcacggcggc
     4741 tccagcgtga agctggtggt gtcggagagc gggtggccgt cgggcggcgg cacggcggcg
     4801 actccggcca acgctaggtt ctacaaccag cacctcatca accacgtcgg gcgcggcacc
     4861 ccacgccacc cgggcgccat cgagacctac atcttcgcca tgttcaacga gaaccagaag
     4921 gacagcggcg tggagcagaa ctggggactc ttctacccca acatgcagca cgtctacccc
     4981 atcaacttct gacggagctc gtgctcgtta agtccctact tgttcttgtt aacgagtaaa
     5041 aagtcatgtt acgcgaactt gacgagctac tcgtttggag agcctcttaa ttacctcctc
     5101 tttccacatg agggatgaga acgtatgagt taataaccag accccattac tgtgaattc
//