From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: E.coli AMS gene
Date: 15 Aug 92 10:01:37 GMT
Distribution: bionet
Checksum: 08107     8

LOCUS       ECOGAMS      4000 bp ds-DNA             BCT       15-AUG-1992
DEFINITION  E.coli AMS gene
ACCESSION   X67470
KEYWORDS    ams gene.
SOURCE      Escherichia coli DNA.
  ORGANISM  Escherichia coli
            Prokaryotae; Gram-negative facultatively anaerobic rods;
            Enterobacteriaceae.
REFERENCE   1  (bases 1 to 4000)
  AUTHORS   Casaregola,S., Jacq,A. and Laoudj,D.
  TITLE     Cloning and analysis of the entire E.coli AMS gene
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
COMMENT     *source: strain=K12;
            From EMBL    entry ECGAMS;  dated 03-AUG-1992.
FEATURES             Location/Qualifiers
     -35_signal      144..150
     -10_signal      167..173
     RBS             529..531
     CDS             541..3618
                     /gene="ams"
                     /codon_start=1
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVVRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAAPVVAPAPKAAPATPARPA
                     QPGLLSRFFGALKALFSGGEETKPTEQPAPKAEAKPERQQDRRKPRQNNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTADEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETVAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEEKNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVHVQPMVTEVPVAAAIEPVVSAPVVEEVAGVVEAPVQVAERQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEQAEPVVEPQEETADIEEVVETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVEPEVTVEITTLPANDARSSTGICSGATASQ"
     terminator      3743..3771
                     /note="rho independant"
BASE COUNT     1034 a   1065 c   1060 g    841 t
ORIGIN
        1 ccggactcat ttttctatgc ttatatttac ttttgcacct tattacttca ctgcgtgatc
       61 actttattga tggttattaa accaatcacc agcaagaagt gaaaaaactg tgagtaagcg
      121 ggtgataaat ggtaaaagtc atcttgctat aacaaggctt gcagtggaat aatgaggccg
      181 tttccgtgtc catccttgtt aaaacaagaa attttacgga ataacccatt ttgcccgacc
      241 gatcatccac gcagcaatgg cgtaagacgt attgatcttt caggcagtta gcgggctgcg
      301 ggttgcagtc cttaccggta gatggaaata tttctggaga gtaataccca gtctgtttct
      361 ttgataattg cgctgttttt ccgcatgaaa aacgggcaac cgacactctg cgcctctttg
      421 agctgacgat aaccgtgagg ttggcgacgc gactagacac gaggccatcg gttcacaccc
      481 ggaaaggcgt tactttgccc gcagcttagt cgtcaatgta agaataatga gtaagttacg
      541 atgaaaagaa tgttaatcaa cgcaactcag caggaagagt tgcgcgttgc ccttgtagat
      601 gggcagcgtc tgtatgacct ggatatcgaa agtccagggc acgagcagaa aaaggcaaac
      661 atctacaaag gtaaaatcac ccgcattgaa ccgagtctgg aagctgcttt tgttgattac
      721 ggcgctgaac gtcacggttt cctcccacta aaagaaattg cccgcgaata tttccctgct
      781 aactacagtg ctcatggtcg tcccaacatt aaagatgtgt tgcgtgaagg tcaggaagtc
      841 attgttcaga tcgataaaga agagcgcggc aacaaaggcg cggcattaac cacctttatc
      901 agtctggcgg gtagctatct ggttctgatg ccgaacaacc cgcgcgcggg tggcatttct
      961 cgccgtatcg aaggcgacga ccgtaccgaa ttaaaagaag cactggcaag ccttgaactg
     1021 ccggaaggca tggggcttat cgtgcgcacc gctggcgtcg gcaaatctgc tgaggcgctg
     1081 caatgggatt taagcttccg tctgaaacac tgggaagcca tcaaaaaagc cgctgaaagc
     1141 cgcccggccc cgttcctgat tcatcaggag agcaacgtaa tcgttcgcgc attccgcgat
     1201 tacttacgtc aggacatcgg cgaaatcctt atcgataacc cgaaagtgct cgaactggca
     1261 cgtcagcata tcgctgcatt aggtcgcccg gatttcagca gcaaaatcaa actgtacacc
     1321 ggcgagatcc cgctgttcag ccactaccag atcgagtcac agatcgagtc cgccttccag
     1381 cgtgaagttc gtctgccgtc tggtggttcc attgttatcg acagcaccga agcgttaacg
     1441 gccatcgaca tcaactccgc acgcgcgacc cgcggcggcg atatcgaaga aaccgcgttt
     1501 aacactaacc tcgaagctgc cgatgagatt gctcgtcagc tgcgcctgcg tgacctcggc
     1561 ggcctgattg ttatcgactt catcgacatg acgccagtac gccaccagcg tgcggtagaa
     1621 aaccgtctgc gtgaagcggt gcgtcaggac cgtgcgcgta ttcaaatcag ccatatttct
     1681 cgctttggcc tgctggaaat gtcccgtcag cgcctgagcc catcactggg tgaatccagt
     1741 catcacgttt gtccgcgttg ttctggtact ggcaccgtgc gtgacaacga atcgctgtcg
     1801 ctctctattc tgcgtctgat cgaagaagaa gcgctgaaag agaacaccca ggaagttcac
     1861 gccattgttc ctgtgccaat cgcttcttac ctgctgaatg aaaaacgttc tgcggtaaat
     1921 gccattgaaa ctcgtcagga cggtgtgcgc tgtgtaattg tgccaaacga tcagatggaa
     1981 accccgcact accacgtcgt gcgcgtgcgt aaaggggaag aaaccccaac cttaagctac
     2041 atgctgccga agctgcatga agaagcgatg gcgctgccgt ctgaagaaga gttcgctgaa
     2101 cgtaagcgtc cggaacaacc tgcgctggca acctttgcca tgccggatgt gccgcctgcg
     2161 ccaacgccag ctgaacctgc cgcgccagtt gtagctccag caccgaaagc tgcaccggca
     2221 acaccagcac gtcctgcaca acctgggctg ttgagccgct tcttcggcgc actgaaagcg
     2281 ctgttcagcg gtggtgaaga aaccaaaccg accgagcaac cagcaccgaa agcagaagcg
     2341 aaaccggaac gtcaacagga tcgtcgcaag cctcgtcaga acaaccgccg tgaccgtaat
     2401 gagcgccgcg acacccgtag tgaacgtact gaaggcagcg ataatcgcga agaaaaccgt
     2461 cgtaatcgtc gccaggcaca gcagcagact gccgagacgc gtgagagccg tcagcaggct
     2521 gaggtaacgg aaaaagcgcg taccgccgac gagcagcaag cgccgcgtcg tgaacgtagc
     2581 cgccgccgta atgatgataa acgtcaggcg caacaagaag cgaaggcgct gaatgttgaa
     2641 gagcaatctg ttcaggaaac cgaacaggaa gaacgtgtac gtccggttca gccgcgtcgt
     2701 aaacagcgtc agctcaatca gaaagtgcgt tacgagcaaa gcgtagccga agaagcggta
     2761 gtcgcaccgg tggttgaaga aactgtcgct gccgaaccaa ttgttcagga agcgccagct
     2821 ccacgcacag aactggtgaa agtcccgctg ccagtcgtag cgcaaactgc accagaacag
     2881 caagaagaga agaatgctga taaccgtgac aacggtggca tgccgcgtcg ttctcgccgc
     2941 tcgcctcgtc acctgcgcgt aagtggtcag cgtcgtcgtc gctatcgtga cgagcgttat
     3001 ccaacccagt cgccaatgcc gttgaccgta gcgtgcgcgt ctccggaact ggcctctggc
     3061 aaagtctgga tccgctatcc aattgtacgt ccgcaagatg tacaggttga agagcagcgc
     3121 gaacaggaag aagtacatgt gcagccgatg gtgactgagg tccctgtcgc cgccgctatc
     3181 gaaccggttg ttagcgcgcc agttgttgaa gaagtggccg gtgtcgtaga agcccccgtt
     3241 caggttgccg aacgccaacc ggaagtggtt gaaacgacgc atcctgaagt gatcgctgcc
     3301 gcggtaactg aacagccgca ggtgattacc gagtctgatg ttgccgtagc ccaggaagtt
     3361 gcagaacaag cagaaccggt ggttgaaccg caggaagaga cggcagatat tgaagaagtt
     3421 gtcgaaactg ctgaggttgt agttgctgaa cctgaagttg ttgctcaacc tgccgcgcca
     3481 gtagtcgctg aagtcgcagc agaagttgaa acggtagctg cggtcgaacc tgaggtcacc
     3541 gttgagataa ccacgctacc ggccaatgac gcgcgctcca gcaccggaat atgttccgga
     3601 gccaccgcgt cacagtgact gcagcgccct acttttgcct tcgaaggtaa aggtgccgca
     3661 ggtggtcata cggcaacaca tcatgcctct gccgctcctg cgcgtccgca acctgttgag
     3721 taataattag ctcaaagtaa tcaagccctg gtaactgcca gggctttttt atttcatctt
     3781 tgaatcaaca tattaaaatt catcgaatgg catccttgct aaccaacaat gcaaaatagg
     3841 caaaaaaatg cccgccccag cgatcccaga gcaggcagac aaaacatacc cagtttcatg
     3901 atatgtttta aagcgaaatt atttgctgag ctggaagagc gacaatccct gcatatcggt
     3961 aaatgcttta tacgatgcct gcaatgccgt ttgctgcatg
//