From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Cavia porcellus procathepsin E mRNA, complete cds.
Date: 4 Sep 92 10:03:12 GMT
Distribution: bionet
Checksum: 51226     4

LOCUS       GPIPROCAT    1326 bp ss-mRNA            ROD       04-SEP-1992
DEFINITION  Cavia porcellus procathepsin E mRNA, complete cds.
ACCESSION   M88653
KEYWORDS    aspartic proteinase; cathepsin E; pro-cathepsin E.
SOURCE      Cavia porcellus adult cDNA to mRNA.
  ORGANISM  Cavia porcellus
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Rodentia; Hystricomorpha; Caviidae; Caviinae.
REFERENCE   1  (bases 1 to 1326)
  AUTHORS   Kageyama,T., Ichinose,M., Tsukada,S., Miki,K., Kurokawa,K.,
            Koiwai,O., Tanji,M., Yakabe,E., Athanuda,S.B. and Takahashi,K.
  TITLE     Gastric procathepsin E and progastricsin from guinea pig:
            Purification molecular cloning of cDNAs, and characterization of
            enzymatic properties, with special reference to procathepsin E
  JOURNAL   J. Biol. Chem. 267, 16450-16459 (1992)
  STANDARD  full staff_entry
FEATURES             Location/Qualifiers
     misc_feature    1..1326
                     /note="cDNA insert in pGP477"
     CDS             45..1220
                     /product="procathepsin E"
                     /codon_start=1
                     /translation="MKTFLLLLLVLLELGQAPGALHRVPLSRRESLRKKLRAQGQLTE
                     LWKSQNLNMDQCSTIQSANEPLINYLDMEYFGTISIGSPPQNFTVIFDTGSSNLWVPS
                     VYCTSPACQTHPVFHPSLSSTYREVGNSFSIQYGTGSLTGIIGADQVSVEGLTVVGQQ
                     FGESVQEPGKTFVHAEFDGILGLGYPSLAAGGVTPVFDNMMAQNLVALPMFSVYMSSN
                     PGGSGSELTFGGYDPSHFSGSLNWVPVTKQAYWQIALDGIQVGDSVMFCSEGCQAIVD
                     TGTSLITGPPGKIKQLQEALGATYVDEGYSVQCANLNMMLDVTFIINGVPYTLNPTAY
                     TLLDFVDGMQVCSTGFEGLEIQPPAGPLWILGDVFIRQFYAVFDRGNNRVGLAPAVP"
     sig_peptide     45..101
                     /codon_start=1
     misc_feature    102..194
                     /note="propeptide"
                     /evidence=EXPERIMENTAL
     mat_peptide     195..1217
                     /product="cathepsin E"
                     /codon_start=1
BASE COUNT      295 a    381 c    350 g    300 t
ORIGIN
        1 ggaagacggg cctctgcagg agaaaagctg ctgctcggac cacgatgaaa acgtttctgc
       61 tgttactgct ggtgctcctg gagctgggac aggccccagg agcccttcac agggtgcccc
      121 tcagccggcg tgagtccctc cggaagaagc tgcgagccca gggacagctc actgagctct
      181 ggaaatccca gaacttgaat atggaccagt gctccaccat ccagagtgcc aatgagcccc
      241 tcatcaacta cctggatatg gagtacttcg gcactatctc cattggctct ccaccacaaa
      301 acttcactgt catcttcgac accggctcct ccaatctctg ggttccctct gtctactgca
      361 ccagcccagc ctgccaaacg caccccgtgt tccacccttc cctgtccagc acctacaggg
      421 aggtggggaa ttctttctcc attcagtacg ggaccgggag cttgactggg atcatcggag
      481 ccgaccaagt ctctgtggaa ggactcactg tggttggcca gcaatttgga gaaagtgtcc
      541 aagaaccggg caagaccttt gtgcatgcag agtttgatgg gattctgggc ctaggctatc
      601 cctccttggc tgctggaggg gtgacccctg tgtttgacaa catgatggcc cagaacctgg
      661 tggctctgcc aatgttttct gtctacatga gcagtaaccc aggtggctca ggcagtgaac
      721 tgaccttcgg aggctatgac ccctcccatt tctcggggag cctgaattgg gtcccagtca
      781 ccaagcaggc ctactggcag attgccctgg atggaatcca ggtgggggac agtgtgatgt
      841 tctgctccga gggctgccag gccattgtgg acacaggaac ttccctcatc actggccctc
      901 ctggaaagat caagcagctg caagaagctc tcggggccac atacgtggac gaaggatatt
      961 ctgtgcagtg tgccaacctc aacatgatgc tggatgtcac tttcatcatc aacggggtcc
     1021 cctacacgct caacccaacc gcctacaccc tgctggactt cgtggacggc atgcaggtct
     1081 gcagcactgg ctttgaagga cttgaaatcc agcctccagc tggacccctc tggatcctcg
     1141 gggatgtctt catccggcag ttctacgccg tctttgatcg tgggaataac cgcgtggggt
     1201 tggccccagc agtcccctga gtagagtttt atgtccctgc cacctgacac ttgtgggctt
     1261 gttaggttgg ggcattgttt acactaaaaa aaaaaaaaat tcattttcag taaaaaaaaa
     1321 aaaaaa
//