From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Zebrafish retinoic acid receptor alpha 2.B (RAR-alpha-2.B) mRNA,
Date: 22 Dec 92 22:57:57 GMT
Distribution: bionet
Checksum: 16709 6

LOCUS       ZEFRARA2B    2539 bp ss-mRNA            VRT       30-NOV-1992
DEFINITION  Zebrafish retinoic acid receptor alpha 2.B (RAR-alpha-2.B) mRNA,
            complete cds.
ACCESSION   L03399
KEYWORDS    retinoic acid receptor-alpha-2-isoform;
            retinoic acid receptor-alpha-2.B-isoform.
SOURCE      Brachydanio rerio gastrula (6-9 hours post fertilization) embryo
            cDNA to mRNA.
  ORGANISM  Brachydanio rerio
            Eukaryota; Animalia; Chordata; Vertebrata; Osteichthyes;
            Actinopterygii; Cypriniformes; Cyprinoidei; Cyprinidae.
REFERENCE   1  (bases 1 to 2539)
  AUTHORS   Stachel,S.E. and Kushner,P.
  TITLE     The molecular characterization of three zebrafish retinoic acid
            receptor genes suggests the retinoic acid pathway functions in
            embryonic hindbrain development
  JOURNAL   Unpublished (1993)
  STANDARD  full automatic
REFERENCE   2  (bases 1 to 2539)
  AUTHORS   Stachel,S.E.
  TITLE     Retinoic acid reprograms RAR expression and shows anteroposterior
            specification occurs in the pre-gastrula zebrafish
  JOURNAL   Unpublished (1993)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     mRNA            1..2539
                     /standard_name="'retinoic acid receptor alpha-2.B'"
                     /gene="RAR-alpha-2.B"
                     /citation=[1]
                     /note="'zebrafish encodes 2 separate RAR alpha-2 genes'"
     misc_feature    188..349
                     /product="'A-domain'"
                     /label=A-domain
                     /note="'type 2 isoform'"
     misc_feature    434..631
                     /function="'DNA binding'"
                     /label=C-domain
     misc_feature    775..1429
                     /function="'ligand binding'"
                     /label=E-domain
     polyA_signal    2518..2523
     polyA_site      2539
     CDS             188..1561
                     /gene="RAR-alpha-2.B"
                     /evidence="EXPERIMENTAL"
                     /citation=[1]
                     /product="retinoic acid receptor alpha-2.B"
                     /codon_start=1
                     /translation="MYESVDVVGLTPSPNPFLSMDYYHQNRGCLIPDKGLVSGAARGF
                     RNPHWSGSNHSVETQSTSSEEIVPSPPSPPPPPRVYKPCFVCQDKSSGYHYGVSACEG
                     CKGFFRRTIQKNMVYTCHREKSCIINKVTRNRCQYCRLQKCLEVGMSKESVRNDRNKR
                     KKDDKKQECLENYVLSPDTEKMIEQVRKAHQETFPSLCQLGKYTTNNSADHRVALDVD
                     LWDKFSELSTKCIIKTVEFAKQLPGFTTLTIADQITLLKAACLDILILRICTRYTPDQ
                     DTMTFSDGLTLNRTQMHNAGFGPLTDLVFAFANQLLPLEMDDAETGLLSAICLLCGDR
                     QDLEQSDKVDELQEPLLEALKIYVRNRRPHKPHMFPKMLMKITDLRSISAKGAERVIT
                     LKMEIPGSMPPLIQEMLENSEGLEGGGSKGAGGGGGGGGKGAPPGSCSPSLSPSSAHS
                     SPSAHSP"
BASE COUNT      674 a    634 c    615 g    616 t
ORIGIN      
        1 gcgagcttct gctgaactga cttacacaaa cacactgaaa gccttcaagt tcttctgcac
       61 gcgtttttta agagacgctc cctgtattgt tttataagag gatccttcgt gtttttgggc
      121 tataatatat gacgtttgga gcctacagct acatttactt tcggagaata ttggctttta
      181 tttcacgatg tacgagagcg tggacgttgt gggactgacc cctagcccga acccgttttt
      241 aagcatggat tactaccacc agaaccgcgg ctgtttgatc ccggataaag gcctcgtgtc
      301 gggagcggcc cgcgggttca ggaaccctca ctggagcggc tcgaatcact ctgtggagac
      361 ccagagcaca agctcagagg agatcgtacc cagccctcct tcaccgccac ctccaccacg
      421 ggtctacaag ccctgttttg tctgccagga caagtcctcg ggctaccact atggagtcag
      481 cgcatgtgaa gggtgtaagg gttttttccg gagaacgatt cagaagaaca tggtgtacac
      541 ctgccacaga gagaagagct gtatcatcaa caaggtcacc cgaaaccgct gccagtactg
      601 ccgactgcag aagtgcctgg aagtgggcat gtctaaagaa tctgttagga acgaccggaa
      661 caaaaggaag aaagacgaca agaagcaaga atgtttggaa aattacgtct taagtccaga
      721 tacggagaag atgatcgagc aggtgaggaa ggcccatcag gaaacctttc cctcgctctg
      781 tcagctggga aaatacacca cgaacaacag cgcagaccac agggtggctc tggatgtcga
      841 cctgtgggac aaatttagcg agctctccac caaatgcatc attaaaacag tggagttcgc
      901 caagcagtta cccggcttca ccacgctcac catcgcagat cagatcaccc tgttaaaggc
      961 cgcctgtctc gacatcctga tcctgcgaat atgtacacgc tacacacccg atcaggacac
     1021 catgacgttt tcagatggtc tgacactgaa ccgcacacag atgcacaacg ccggctttgg
     1081 ccctctcaca gatctggtgt tcgctttcgc caaccagctt ctgcctctgg agatggatga
     1141 tgcagagact gggcttctta gcgccatctg tctgctgtgt ggagatcggc aggatctaga
     1201 gcagtctgat aaggtggatg aacttcagga gcccttgctg gaggccctga agatctatgt
     1261 gagaaatcgg agacctcata aaccacacat gttccccaag atgctgatga agatcacaga
     1321 cctgaggagc atcagcgcta aaggagcgga gcgagtaata acgctgaaaa tggagatccc
     1381 aggctccatg cctccgctca ttcaggagat gctggaaaat tcagagggtc tggagggagg
     1441 cgggtcgaaa ggagctggag gaggaggtgg tggcggtgga aagggagctc caccaggaag
     1501 ctgcagcccc agtttgtctc ccagttcggc acacagcagc ccctcggctc actctccttg
     1561 actttcatct ccagacagac ctccatacag aattaaactg gttcctaacc agaaataagc
     1621 cacagccgac cagcgccaga gagcagattc cgtagacttt gacccgactg aacagtaact
     1681 ctggccagct ttcattcgct gaagtccagt agagttacac acaggctgag cctgatcaaa
     1741 actcacgccg tcattaaaac ctccagtgat tcagcgacta caagagcctt ttcacgtggc
     1801 gctgcttcac agagatgaca gaacaaacgc cctgtgagcg caacgctctt tactgtgtct
     1861 tcccggctct gctcccatca cgcgaactgt gaaggcccag ggaagctcgt ctgaaggacg
     1921 cgttctctcg gcctcagcgt tatttataaa cttcaatccc ttaaagcaag ctaaaggact
     1981 ttaggacgtc agctgtccac aagtcaggac attttgaact ttccagctgt tttggttctg
     2041 tttctttgtt ttagtacaaa agatatatga atatatgaaa tctatattga cagaaatgag
     2101 atttgactct tttggtctta ggtggaatgc agaacatttc ttatcttttt ctctcacttt
     2161 ttgtctcaag agttgaacat ggttatggtc gacccactgt taccttcact ccaagacagt
     2221 cctgttcacg tcacgcacaa ggcacgtcgc actgaaaatg atgtgtgcgc ttcaatcaag
     2281 atgggggtaa aacacagttt tgtccggacg atttggagat atgacacttc atcagaaatg
     2341 aaggatcccc tgtaaacaag cggcttgtga ttatgatgtg tgtgagatct cggttttaac
     2401 ggagtggcga atgcttcctg tagcttttaa tattgctttt ttttgctttt cattttgtaa
     2461 ttgtcacctg tcgaaagaca cagagacccc cttctaaaca gtattagctg attacaaaat
     2521 aaaagagatt tccaaaaat
//