From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Brachydanio rerio ependymin beta and gamma chains (Epd) gene,
Date: 29 Aug 92 10:02:27 GMT
Distribution: bionet
Checksum: 47792     9

LOCUS       ZEFEPEN      4308 bp ds-DNA             VRT       29-AUG-1992
DEFINITION  Brachydanio rerio ependymin beta and gamma chains (Epd) gene,
            complete cds.
ACCESSION   M89643
KEYWORDS    ependymin.
SOURCE      Brachydanio rerio (library: lambda EMBL3) adult DNA.
  ORGANISM  Brachydanio rerio
            Eukaryota; Animalia; Chordata; Vertebrata; Osteichthyes;
            Actinopterygii; Cypriniformes; Cyprinoidei; Cyprinidae.
REFERENCE   1  (sites)
  AUTHORS   Sterrer,S., Koenigstorfer,A. and Hoffmann,W.
  TITLE     Biosynthesis and expression of ependymin homologous sequences in
            zebrafish brain
  JOURNAL   Neuroscience 37, 277-284 (1990)
  STANDARD  full staff_review
REFERENCE   2  (bases 1 to 4308)
  AUTHORS   Rinder,H., Bayer,T.A., Gertzen,E. and Hoffmann,W.
  TITLE     Molecular analysis of the ependymin gene and functional test of its
            promoter region by transient expression in Brachydanio rerio
  JOURNAL   DNA Cell Biol. 11, 425-432 (1992)
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     mRNA            join(2033..2155,2297..2331,3067..3201,3317..3503,
                     3601..3685,3765..3964)
                     /note="alternative"
                     /gene="Epd"
     mRNA            join(2033..2155,2297..2331,3067..3201,3317..3503,
                     3601..3685,3765..4086)
                     /note="alternative"
                     /gene="Epd"
     CDS             join(2068..2155,2297..2331,3067..3201,3317..3503,
                     3601..3685,3765..3888)
                     /note="beta and gamma chains"
                     /product="ependymin"
                     /gene="Epd"
                     /codon_start=1
                     /translation="MHTVKLLCVVFSCLCAIGWASHHSHRQPCHSPQLTSGTMKVVST
                     GGHDLASGEFSYDSKTNKFRFVEDTTHANKTSYMDVLIHFEEGVLYEIDSKNESCKKE
                     TLQFRKHLMEIPVDATHESESYMGSPSLTEQGLRVRVWNGKFPELHAHYSLSTTSCGC
                     LTVSGSYYGEKKDLFFSFFGVETEVDDLQVFAPPAYCEGVSFEEAPDDHSFFDLFHD"
     TATA_signal     2004..2010
                     /gene="Epd"
     exon            2033..2155
                     /number=1
                     /gene="Epd"
     intron          2156..2296
                     /number=1
                     /gene="Epd"
     exon            2297..2331
                     /number=2
                     /gene="Epd"
     intron          2332..3066
                     /number=2
                     /gene="Epd"
     exon            3067..3201
                     /number=3
                     /gene="Epd"
     intron          3202..3316
                     /number=3
                     /gene="Epd"
     exon            3317..3503
                     /number=4
                     /gene="Epd"
     intron          3504..3600
                     /number=4
                     /gene="Epd"
     exon            3601..3685
                     /number=5
                     /gene="Epd"
     intron          3686..3764
                     /number=5
                     /gene="Epd"
     exon            3765..3964
                     /number=6a
                     /note="alternative"
                     /gene="Epd"
     exon            3765..4086
                     /number=6b
                     /note="alternative"
                     /gene="Epd"
     polyA_signal    3939..3944
                     /note="alternative"
     polyA_signal    4066..4071
                     /note="alternative"
BASE COUNT     1282 a    759 c    820 g   1447 t
ORIGIN
        1 tctagagagg gttcagtatc aactactaaa caccatcatg gtaacaaaca ttatggaaac
       61 attctgttaa ccactgtaac aaattttaac tgtagctttt tttcagtaaa ctatttcgta
      121 attagaatca tggatttatt tttaacaaaa tgtgcaaaat atggacaaac atgaatgtaa
      181 aaagtaaaaa caaatggcca agtggttgag tttgtccata ttttactcaa atttaaattg
      241 aaaaaaactg ctatatatta aaatgcgcca actttttaaa attaaaatta caaatattct
      301 aaaagctgtt gatatgaaat tcaaccacat ttttataaaa acccaaacag tacaacataa
      361 aaacaaaaca aaataaatct tgaaaatttt gttatgtgtc gccaccgcag gcgacgcagt
      421 ggcgcagtag gttgtgctgt cgcctcacag caagaaggtc gctggttcga gcctcggctg
      481 ggtcagttgg tgtttctgtg tggagtttgc atgttctccc tgcgtttgcg tgggtttcct
      541 ccaggtgctc cggtttccct cacattccaa agacatgtgg tacaggtgaa ttgggttggc
      601 taaaatgtaa agtgtatggt gtgcgaatat ggtgtgcgaa taagtgtgta cgggtttccc
      661 agtgatgggt tgcggctgga agggcatccg ctgcataaaa catatgctgg ttaattccac
      721 tgtggcgacc ccagattaat aaagggacta agccaaaaag aaaataaatg aatgtataac
      781 aatgtaatga cacaaggaga aagtactgaa atactttggt gatagctgct taaagactcc
      841 tctcatatgg agaatgaggt cacatgcgtt gatcacgtgt gatttttttt tttcacagac
      901 ttcaacagat tgtacatttc ttgatggttc tgtgtctggt ctcgtctatc aaatctgatt
      961 tttaatttgt attttctatt ggattcaagt caggtgattg gctggaccat tctacagctt
     1021 gattttcttt ctctgaaagc atttgagagt ttccttggct gtgttttgga tcattgtctc
     1081 actgaaatgt ccactctgtt atcatcttta tcatcctgat gatataaatg ttagagtgaa
     1141 gcagctgata ataattgaca atgacaaaga actgctgaga gatttcagct gctgtctggg
     1201 ctttcactgt ctttctacgc cttcattctt catgtgttca aaactttagt cctgtgtcat
     1261 ttcattttat tacaaatagc ttaatttata agtaattaat attattttat ttgcagatat
     1321 tgatttattg gttgttacca acatctggtg taaatttcaa gtcagcggca ccttagaaat
     1381 atcttttctg agaaaaatgg tgaagtgttg aatacttctt ttaccctgct gtatataaac
     1441 agaaattacc acagtaattt ccaatgaaaa tcaaagtgct agtctctgca accagactgc
     1501 aaagtaagtg acactacaga attgtctttg attaaagttt tccctttgaa ttttttaggt
     1561 ttattgcaca gtttatgtaa tcttacttaa agtgtgattt ctaatagaag agctgaatga
     1621 atgaggaaag taccagatgg tgattgtttt cacacctgca taacccactt ttgtttttgt
     1681 tttgtttaat ttttttcttg attgagaagt ttagtcagca taatcttcac cttacatgaa
     1741 ccacaatcat gcgacatgac accaactgtc tctggtccac cagtatgaca ccagcacccc
     1801 tgagcgccga tcagaggccg gatggggcga ttgtttgttg aaatgtactt tttttattta
     1861 gtggacagtc atttgtgcca attttccagc tttctcattt gggcttacga tagtaggtgt
     1921 tttgaaacat cagatgttta ttctattgct ggacgtccag aggcagaggg gagggggcat
     1981 ttagatgggg tgcaggggtt atgtataaaa ggcagagtct gaatgagggt ggaagtgtct
     2041 ggacctgaag aaagttaaag agcaacgatg catacagtca agctgctctg tgtggtgttc
     2101 tcttgcctct gcgccatcgg ctgggcttct catcactctc atcggcagcc atgtcgtaag
     2161 tgcttaattt ttttttaaag tagtttcttt aatattgctg cattttctca attaaattgg
     2221 tccaaaatga gtatgacaat gtttttaaga ggctgctgac aaggcataaa tgtcatctat
     2281 ctcgtttctt ttacagattc accacagctg accagtggaa cgatgaaagt ggtgagtgaa
     2341 caacatttta aagacatgat tgatgatttt agaagtgcaa tattactgtg caagaagaac
     2401 gctttaaaaa atgctttgtc ttgcaaacaa aagcaaggcc tgatttattc actgattcac
     2461 tgtaaacaat ctaattcagt aaagagtgca cagattttcc gattgtacat aaactacacc
     2521 gtaaaattat ggttgtctta aattttgaaa ctggattaat aaattaacct tatgagtcat
     2581 tgacgatatt atgttaactg acttaaactg caagcataac ttatacaatt aagttagaac
     2641 atgattgtct tggttttata agttgcaatg aactaaaaac atatgttgtc atgactatgt
     2701 aatcatatat ttttttacag tgtaggctct agaaaataaa tgtcatttca acatacattt
     2761 gggtaaaata tttacaagtt aacaaatggg tttcagtttt tttttattta gtttttgaaa
     2821 aaaaaaaaac aaccaaagat gtaacctagt gattgggttt gtccatattt tccccaaaac
     2881 tgtgttgaaa caacccatca tttctttttt aaagttattt aaagtgttaa tatttgtttt
     2941 actatgaact ttaacttgca tttacaaatg ttgtttgtgt ttcagaacat gcctaacact
     3001 aaccattact gtataactga acactttcaa tcttctcttt tgttcacgtt ctaaacacac
     3061 attcaggttt ccacaggggg tcatgacctt gcatctggag agttcagtta tgactccaaa
     3121 acgaataaat ttcgttttgt tgaggacacc actcacgcga acaaaacttc ttatatggat
     3181 gtgctcatac attttgaaga ggtgggacga ctcagcaact ctctctctct attcctcttc
     3241 ctctttcagc ttgtgactca ccagttttgt tgatgctttt tacagtcaaa acattaaaga
     3301 attttttttc gcacagggtg tactctatga gatagacagt aaaaatgaga gttgcaagaa
     3361 ggagactctg cagttccgca agcacctgat ggagattccc gttgatgcca ctcacgagtc
     3421 tgagagttac atgggtagcc cctctctcac cgagcaggga ctcagagttc gtgtgtggaa
     3481 cggaaagttt cctgaacttc acggtcctta ctttttgtac ccctattcgg ttaattgatt
     3541 gcttttatgt ataaaattaa agaaagcatg tttacttttt gagtttctga tttttttcag
     3601 ctcactactc tctgtctacc acctcctgtg gctgtttgac ggtctctggc tcctactatg
     3661 gcgagaagaa agatcttttc ttcaggtatt caaacagcgt tctttacatg ttattgtttc
     3721 agctttgtat gccagtcagt acattgtgct gttttctgct ttagcttctt cggtgttgaa
     3781 acagaagttg acgaccttca agtatttgca cctccggcct attgtgaggg tgtgtcattt
     3841 gaggaggctc cagacgatca cagcttcttc gacctgttcc acgactgaag aaaacacaca
     3901 agatgacctg caaactgcca acaatggcta catacaataa taaaaagagc atttaattac
     3961 aaatatttcc tgtatccatc tctctttctt cagaaaatgc ttgcactttc tatcttacct
     4021 tctttttttg tcttttgcaa tttgtttctg cactctaatg aagtcaataa agatgagctt
     4081 tcgctgaaat ccttcttgat gtttgtttgt aaattgaaaa taatcacaaa gctatgtgaa
     4141 caaagggtcc ttcaaacaac aacaattcta aaactttgga aaaagggaaa aaagttacta
     4201 aaaactgcat acacaaagga aaatgaacag aattaactaa taaactaagg gtgttttgat
     4261 gtaacataca atagtttaaa tgtgtgtaat aattggatgg aggaaaaa
//