HDTenEightyP/NetNews huggingface dataset:
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Lentinula edodes ribosomal RNA (6LR6) large subunit, partial.
Date: 19 Aug 92 10:01:24 GMT
Distribution: bionet
Checksum: 03678     2

LOCUS       LELRRLSC      177 bp ss-rRNA            RNA       19-AUG-1992
DEFINITION  Lentinula edodes ribosomal RNA (6LR6) large subunit, partial.
ACCESSION   M98610
KEYWORDS    large subunit ribosomal RNA.
SOURCE      Lentinula edodes (library: Duke culture collection 607) cDNA to
            rRNA.
  ORGANISM  Lentinula edodes
            Eukaryota; Fungi; Amastigomycota; Basidiomycetes.
REFERENCE   1  (bases 1 to 177)
  AUTHORS   Hibbett,D.S. and Vilgalys,R.
  TITLE     Phylogenetic relationships of the basidiomycete genus Lentinus
            inferred from molecular and morphological characters
  JOURNAL   Unpublished (1992)
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     rRNA            <1..>177
                     /note="Approximately aligns to region between positions
                     845 to 1093in Saccharomyces cerevisiae large subunit
                     nuclear rRNA."
                     /product="large subunit ribosomal RNA"
                     /citation=[1]
BASE COUNT       50 a     30 c     42 g     55 t
ORIGIN
        1 tttgggtata gggagctaat cgaacatcta gtagctggtt cctgccgaag tttccctcag
       61 gatagcaaac tcattgtcag atttatgtgg taaaggaatg attagaggcc ttggggttgt
      121 aacaacctta acctattctc aaactttaaa tatgtaagag agccgtttta attgggc
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Homo sapiens DNA-binding protein (ZNF) gene, partial cds.
Date: 21 Aug 92 10:11:55 GMT
Distribution: bionet
Checksum: 62551     2

LOCUS       HUMZNFM        48 bp ds-DNA             PRI       21-AUG-1992
DEFINITION  Homo sapiens DNA-binding protein (ZNF) gene, partial cds.
ACCESSION   M88369
KEYWORDS    DNA binding protein; zinc finger protein.
SOURCE      Homo sapiens Placenta DNA.
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 48)
  AUTHORS   Bray,P.L., Lichter,P., Thiesen,H.J., Ward,D.C. and Dawid,I.B.
  TITLE     Characterization and mapping of human genes encoding zinc finger
            proteins
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 88, 9563-9567 (1991)
  STANDARD  full automatic
REFERENCE   2  (bases 1 to 48)
  AUTHORS   Lichter,P., Bray,P., Ried,T., Dawid,I.B. and Ward,D.C.
  TITLE     Clustering of C2-H2 zinc finger motif sequences within telomeric
            and fragile site regions of human chromosomes
  JOURNAL   Genomics 13, 999-1007 (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     CDS             1..48
                     /standard_name="zinc finger motif"
                     /product="DNA binding protein"
                     /gene="ZNF"
                     /note="putative"
                     /codon_start=1
                     /translation="IHTGEKPFSCRECSKA"
                     /partial
BASE COUNT       10 a     15 c     17 g      6 t
ORIGIN
        1 atccacacgg gggagaagcc cttctcgtgc cgggagtgca gcaaggcc
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Epulopiscium sp. ribosomal RNA.
Date: 22 Aug 92 10:00:26 GMT
Distribution: bionet
Checksum: 38007     3

LOCUS       EPURRNAC     1442 bp ss-rRNA            RNA       22-AUG-1992
DEFINITION  Epulopiscium sp. ribosomal RNA.
ACCESSION   M99574
KEYWORDS    ribosomal RNA.
SOURCE      Epulopiscium sp. (strain morphotype B) rRNA.
  ORGANISM  Epulopiscium sp.
            Prokaryotae; Gram-positive bacteria.
REFERENCE   1  (bases 1 to 1442)
  AUTHORS   Angert,E.R., Clements,K.D. and Pace,N.R.
  TITLE     The Largest Bacterium: A Phylogenetic Analysis of Epulopiscium-like
            Surgeonfish Symbionts
  JOURNAL   Unpublished (1992)
  STANDARD  full staff_entry
FEATURES             Location/Qualifiers
     rRNA            1..1442
BASE COUNT      397 a    311 c    430 g    304 t
ORIGIN
        1 gatgaacgct ggcggcgtgc ttaacacatg caagtcgaac ggacttggga gcttgctctt
       61 aagttagtgg cggacgggtg agtaacacgt gggtaacctg ccctatagaa tggaataacg
      121 tttggaaaca gacgctaata ccatataacc taattaggtc gcatgacttg gttagcaaag
      181 attttatcgc tataggatgg acctgcgttg gattagctag ttggtgagat aaaagctcac
      241 caaggcgacg atccatagcc ggcctgagag ggtgaacggc cacactggga ctgagacacg
      301 gcccagactc ctacgggagg cagcagtggg gaatattgca caatgggcgc aagcctgatg
      361 cagcgacgcc gcgtgaagga tgaaggtttt cggatcgtaa acttctatca gcagggaaga
      421 aaaaaatgac ggtacctgac taagaagccc cggctaacta cgtgccagca gccgcggtaa
      481 tacgtagggg gcgagcgtta tccggaatta ctgggtgtaa agggtgtgta ggcggcatgg
      541 taagtcagat gtgaaaactt ggggctcaac ttcaagattg catctgaaac tgctaagcta
      601 gagtgcagga gaggaaagtg gaattccaag tgtagcggtg aaatgcgtag agatttggag
      661 gaacaccagt agcgaaggcg actttctgga ctgtaactga cgctgagaca cgaaagcgtg
      721 gggagcgaac aggattagat accctggtag tccacgccgt aaacgatgaa tactaggtgt
      781 cgggccttac ggggttcggt gccgcagcta acgcattaag tattccacct gggaagtacg
      841 accgcaaggt tgaaactcaa aggaattgac ggggacccgc acaagcggtg gagcatgtgg
      901 tttaattcga agcaacgcga agaaccttac ctaaacttga catccttatg atcggagggt
      961 aatgcctctt tctctagctt gctagggcat aagtgacagg tggtgcatgg ttgtcgtcag
     1021 ctcgtgtcgt gagatgttgg gttaagtccc gcaacgagcg caacccttat cttcagtagc
     1081 catcatttag ttgggcactc tggagagact gccaaggaca acttggagga aggtggggat
     1141 gacgtcaaat catcatgccc cttatgttta gggctacaca cgtgctacaa tggctgctac
     1201 aaagggaagc atagcggtaa cgtgaagcaa accccaaaaa agcagtccca gttcggattg
     1261 tggtctgcaa ctcgaccaca tgaagttgga atcgctagta atcgcgaatc agaatgtcgc
     1321 ggtgaatacg ttcccgggtc ttgtacacac cgcccgtcac accatggaag ttgagggggc
     1381 ccaacgtcag tgacctaacc gcaaggaggg agctgcctaa ggcaaaatca atgactgggg
     1441 tg
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Rana catesbeiana Trp- Ala- Asn- Cys- and Tyr-tRNA genes and
Date: 13 Aug 92 10:00:24 GMT
Distribution: bionet
Checksum: 15313     3

LOCUS       FRGMTDRA      457 bp ds-DNA             ORG       13-AUG-1992
DEFINITION  Rana catesbeiana Trp- Ala- Asn- Cys- and Tyr-tRNA genes and
            cytochrome oxidase I gene, 5' end.
ACCESSION   M57725
KEYWORDS    cytochrome oxidase subunit 1; transfer RNA-Ala; transfer RNA-Asn;
            transfer RNA-Cys; transfer RNA-Trp; transfer RNA-Tyr.
SOURCE      Rana catesbeiana mitochondrial DNA.
  ORGANISM  Mitochondrion Rana catesbeiana
            Eukaryota; Animalia; Chordata; Vertebrata; Amphibia; Lissamphibia;
            Anura; Neobatrachia; Ranoidea; Ranidae.
REFERENCE   1  (bases 1 to 457)
  AUTHORS   Yoneyama,Y.
  TITLE     The nucleotide sequences of the heavy and light strand replication
            origins of the Rana catesbeiana mitochondrial genome
  JOURNAL   J. Nippon Med. Sch. 54, 429-440 (1987)
  STANDARD  simple staff_review
REFERENCE   2  (bases 1 to 457)
  AUTHORS   Yoneyama,Y.
  TITLE     The nucleotide sequences of the heavy and light strand replication
            origins of the Rana catesbeiana mitochondrial genome
  JOURNAL   Nippon Ika Daigaku Zasshi 54, 429-440 (1987)
  STANDARD  simple staff_review
FEATURES             Location/Qualifiers
     CDS             <1..63
                     /gene="URF 2"
                     /codon_start=1
                     /translation="AIMNTMALILLPITPTLLLL"
     tRNA            64..133
                     /anticodon=(pos:95..97,aa:Trp)
                     /product="transfer RNA-Trp"
     tRNA            complement(134..203)
                     /anticodon=(pos:170..172,aa:Ala)
                     /product="transfer RNA-Ala"
     tRNA            complement(204..258)
                     /anticodon=(pos:241..243,aa:Asn)
                     /product="transfer RNA-Asn"
     tRNA            complement(304..368)
                     /anticodon=(pos:339..341,aa:Cys)
                     /product="transfer RNA-Cys"
     tRNA            complement(369..435)
                     /anticodon=(pos:405..407,aa:Tyr)
                     /product="transfer RNA-Tyr"
     CDS             440..>457
                     /product="cytochrome oxidase subunit I"
                     /codon_start=1
                     /translation="MFTRWF"
BASE COUNT      124 a    127 c     81 g    125 t
ORIGIN
        1 gctattataa ataccatggc acttatcctt cttcccatta ccccaactct ccttctctta
       61 taaagaaact taggctagca cagaccaaag gccttcaaag ccttaagcgg aggttaaact
      121 ccttcagttt ctgtaggact tgcaggatat taacctacat ctcctgaatg caactcaggc
      181 cctttaaatt aagataaagc cctctagaat gacgggcctc gatcccgtaa tattttagtt
      241 aacagctaaa cactctatcc agcgagcttc attctacttc tcccgcttat aaaaaaacgg
      301 gagaagcccc ggcaggtatt atactgcatc ttggggtttg caaccccacg tgttgacacc
      361 ccaaggcctg gtaaagaggg gactcaaacc tctgtcttcg aagctacaat tcgccgcctg
      421 ctcggccact ttacctgtga tattcacccg ttgattt
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human endothelin (ETN) gene, complete cds.
Date: 22 Aug 92 10:00:56 GMT
Distribution: bionet
Checksum: 22910     3

LOCUS       HUMETN1       682 bp ds-DNA             PRI       22-AUG-1992
DEFINITION  Human endothelin (ETN) gene, complete cds.
ACCESSION   M25377 J04819 J05489
KEYWORDS    endothelin.
SEGMENT     1 of 4
SOURCE      Human leukocyte DNA and human umbilical vein endothelial cell cDNA
            to mRNA.
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 682)
  AUTHORS   Bloch,K.D., Friedrich,S.P., Lee,M.-E., Eddy,R.L., Shows,T.B.Jr. and
            Quertermous,T.
  TITLE     Structural organization and chromosomal assignment of the gene
            encoding endothelin
  JOURNAL   J. Biol. Chem. 264, 10851-10857 (1989)
  STANDARD  simple staff_review
REFERENCE   2  (bases 2 to 421)
  AUTHORS   Lee,M.-E., Bloch,K.D., Clifford,J.A. and Quertermous,T.
  TITLE     Functional analysis of the endothelin-1 gene promoter: Evidence for
            an endothelial cell-specific cis-acting sequence
  JOURNAL   J. Biol. Chem. 265, 10446-10450 (1990)
  STANDARD  simple staff_review
COMMENT     Draft entry and computer readable copy of sequence [1] kindly
            submitted by K.D.Bloch (06-AUG-89).
FEATURES             Location/Qualifiers
     exon            <519..582
                     /number=1
                     /note="endothelin prepropeptide"
                     /gene="EDN1"
     prim_transcript 252..>682
                     /note="ETN mRNA and introns (5' end put.)"
                     /note="putative"
     intron          583..>682
                     /note="ETN intron A"
BASE COUNT      121 a    178 c    169 g    213 t      1 others
ORIGIN      Chromosome 6.
        1 ggcaggttta gcaaaggtct ctaatgggna ttttattttt cttagccctg cccccgaatt
       61 gtcagacggc ggcgtctgcc tctgaagtta gcagtgattt ctttcgggcc tggccttatc
      121 tccggctgca cgttgcctgt tggtgactaa taacacaata acattgtctg gggctggaat
      181 aaagtcggag ctgtttaccc ccactctaat aggggttcaa tataaaaagc cggcagagag
      241 ctgtccaagt cagacgcgcc tctgcatctg cgccaggcga acgggtcctg cgcctcctgc
      301 agtcccagct ctccaccgcc gcgtgcgcct gcagacgctc cgctcgctgc cttctctcct
      361 ggcaggcgct gcttttctcc ccgttaaagg gcacttgggc tgaaggatcg ctttgagatc
      421 tgaggaaccc gcagcgcttt gagggacctg aagctgtttt tcttcgtttt cctttgggtt
      481 cagtttgaac gggaggtttt tgatcccttt ttttcagaat ggattatttg ctcatgattt
      541 tctctctgct gtttgtggct tgccaaggag ctccagaaac aggtaggcac gctcgttgac
      601 ttgtaagtct cggaattaca agttagtgtg ttcttatcca ccttcatgct tttcttgctt
      661 ctatttttcc ccgttctttt ta
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human Ig germline H-chain gene V region, partial cds.
Date: 22 Aug 92 10:00:37 GMT
Distribution: bionet
Checksum: 32945     2

LOCUS       HUM3IGVH      434 bp ds-DNA             PRI       22-AUG-1992
DEFINITION  Human Ig germline H-chain gene V region, partial cds.
ACCESSION   M99399
KEYWORDS    immunoglobulin heavy chain subgroup VH-III;
            immunoglobulin heavy chain variable region.
SOURCE      Homo sapiens (individual_isolate LS) blood DNA.
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 434)
  AUTHORS   Adderson,E.E., Carroll,W.L. and Shackelford,P.G.
  TITLE     The human VH3b gene subfamily is highly polymorphic
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     CDS             join(1..13,117..434)
                     /product="immunoglobulin heavy chain variable region"
                     /gene="IGHV@"
                     /codon_start=1
                     /translation="AIFKGVQCEVQLVESAGALVQPGGSLRLSCAASGFTCSNAWMSW
                     VRQAPGKGLEWVGRIKSKANGGTTDYAAPVKGRFTISRVDSKNTLYLQMNSLKTEDTA
                     VYYCTTDT"
                     /partial
     sig_peptide     1..13
                     /gene="IGHV@"
                     /codon_start=1
                     /partial
     intron          14..116
                     /gene="IGHV@"
BASE COUNT      109 a     89 c    133 g    103 t
ORIGIN
        1 gctattttta aaggtgactt atggagagct agagagattg agtgtgagtg gacatgagtg
       61 agagaaacag tggatatgtg tggcagtttc tgaccttagt gtctctgtgt ttgcaggtgt
      121 ccagtgtgag gtgcagctgg tggagtctgc cggagccttg gtacagcctg gggggtccct
      181 tagactctcc tgtgcagcct ctggattcac ttgcagtaac gcctggatga gctgggtccg
      241 ccaggctcca gggaaggggc tggagtgggt tggccgtatt aaaagcaaag ctaatggtgg
      301 gacaacagac tacgctgcac ctgtgaaagg cagattcacc atctcaagag ttgattcaaa
      361 aaacacgctg tatctgcaaa tgaacagcct gaaaaccgag gacacagccg tgtattactg
      421 taccacagac acag
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Pleurotus eryngii ribosomal RNA (20LR0R) large subunit, partial.
Date: 19 Aug 92 10:03:21 GMT
Distribution: bionet
Checksum: 27783     2

LOCUS       POSRRLSC      234 bp ss-rRNA            RNA       19-AUG-1992
DEFINITION  Pleurotus eryngii ribosomal RNA (20LR0R) large subunit, partial.
ACCESSION   M98649
KEYWORDS    large subunit ribosomal RNA.
SOURCE      Pleurotus eryngii (library: Duke culture collection 625) cDNA to
            rRNA.
  ORGANISM  Pleurotus eryngii
            Eukaryota; Fungi; Eumycota; Basidiomycotina; Hymenomycetes;
            Agaricales; Tricholomataceae.
REFERENCE   1  (bases 1 to 234)
  AUTHORS   Hibbett,D.S. and Vilgalys,R.
  TITLE     Phylogenetic relationships of the basidiomycete genus Lentinus
            inferred from molecular and morphological characters
  JOURNAL   Unpublished (1992)
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     rRNA            <1..>234
                     /note="Aligns to region between positions 70 to 306
                     inSaccharomyces cerevisiae large subunit nuclear rRNA."
                     /product="large subunit ribosomal RNA"
                     /citation=[1]
BASE COUNT       58 a     47 c     69 g     60 t
ORIGIN
        1 ttcccctagt aactgcgagt gaagcgggaa aagctcaaat ttaaaatctg gtggtctttg
       61 gccatccgag ttgtaatcta gagaagtgtt atccgcgctg gaccgtgtac aagtcccctg
      121 gaatggggcg tcatagaggg tgagaatccc gtctttgaca cggactacca gggctttgtg
      181 atgcactctc aaagagtcga gttgtttggg aatgcagctc aaaatggggt ggta
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: A.thaliana mRNA (clone ACaM6) for calmodulin.
Date: 15 Aug 92 10:01:10 GMT
Distribution: bionet
Checksum: 20175     3

LOCUS       ATHCMACAM6    660 bp ss-RNA             PLN       15-AUG-1992
DEFINITION  A.thaliana mRNA (clone ACaM6) for calmodulin.
ACCESSION   Z12024
KEYWORDS    calmodulin.
SOURCE      Arabidopsis thaliana RNA.
  ORGANISM  Arabidopsis thaliana
            Eukaryota; Plantae; Embryobionta; Magnoliophyta; Magnoliopsida;
            Dilleniidae; Capparales; Brassicaceae.
REFERENCE   1  (bases 1 to 660)
  AUTHORS   Gawienowski,M.C.
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
REFERENCE   2  (bases 1 to 660)
  AUTHORS   Gawienowski,M.C., Szymanski,D., Perera,I.Y. and Zielinski,R.E.
  TITLE     Calmodulin Isoforms in Arabidopsis encoded by multiple divergent
            mRNAs
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
COMMENT     *source: strain=Columbia; *source: tissue=leaf; *source:
            clone_library=lambda gt10; *source: clone=ACaM6; *source:
            is_macronuclear=N; *source: is_proviral=N; *source: is_germline=N;
            From EMBL    entry ATCMACAM6;  dated 06-AUG-1992.
FEATURES             Location/Qualifiers
     5'UTR           1..37
     CDS             38..487
                     /product="calmodulin"
                     /codon_start=1
                     /translation="MADQLTDDQISEFKEAFSLFDKDGDGCITTKELGTVMRSLGQNP
                     TEAELQDMINEVDADGNGTIDFPEFLNLMARKMKDTDSEEELKEAFRVFDKDQNGFIS
                     AAELRHVMTNLGEKLSDEEVDEMIREADVDGDGQINYEEFVKVMMAK"
     5'UTR           488..660
BASE COUNT      203 a    109 c    163 g    182 t      3 others
ORIGIN
        1 aaaaatctgg gatttctaaa aaaaggtaag aaggaaaatg gcggatcagc tcaccgatga
       61 ccagatctca gagttcaagg aagcctttag cctcttcgat aaggatggtg atggttgtat
      121 caccaccaag gagctcggaa ccgtgatgag gtctctgggg caaaacccaa cagaagctga
      181 gcttcaagac atgatcaatg aagttgatgc agatgggaac gggaccattg atttcccgga
      241 gttcctgaac ctgatggcaa ggaaaatgaa ggacactgat tcagaggaag agctcaagga
      301 agcttttagg gtttttgata aagaccagaa tggtttcata tctgcagcag agcttcgaca
      361 tgtcatgaca aatctcggtg agaagctatc cgatgaggaa gttgatgaga tgatccgaga
      421 agcggatgtt gatggcgatg gtcagattaa ctatgaagaa ttcgtcaaag tcatgatggc
      481 taagtgannn ttttatctta agcccacaaa aagaaacaag atttatgatt tctacagtga
      541 agttacatta tgacttgatt ctggttgcgt tctttaggac atcattttgc ttatttccct
      601 tgtttgtttg gttaagcctt ttagtgttat gtgaagtatg aactcatatt ttgctcaaaa
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: H.sapiens dystrophin gene, exon 73.
Date: 20 Aug 92 10:03:15 GMT
Distribution: bionet
Checksum: 23959     2

LOCUS       HUMDYST14     214 bp ds-DNA             PRI       20-AUG-1992
DEFINITION  H.sapiens dystrophin gene, exon 73.
ACCESSION   M86897
KEYWORDS    Becker muscular dystrophy; Duchenne muscular dystrophy; dystrophin;
            integral membrane glycoprotein; membrane-associated protein.
SOURCE      Homo sapiens DNA.
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 214)
  AUTHORS   Roberts,R.G., Coffey,A.J., Bobrow,M. and Bentley,D.R.
  TITLE     Determination of the exon structure of the distal protion of the
            dystrophin gene by vectorette PCR
  JOURNAL   Genomics 13, 942-950 (1992)
  STANDARD  simple staff_entry
FEATURES             Location/Qualifiers
     exon            51..116
                     /number=73
                     /gene="dystrophin"
BASE COUNT       71 a     33 c     42 g     68 t
ORIGIN      chromosome X, map position Xp21
        1 gattctaaga cgtcacataa gttttaatga gcttttacgt tttttatcag gctagcagaa
       61 atggaaaaca gcaatggatc ttatctaaat gatagcatct ctcctaatga gagcatgtaa
      121 gtatcccatc tctttttaca aaatgttcct gacaatgaaa ttgctttgag ggatttagag
      181 gtaggatagc acaggatata ggaattagca tcat
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human chromosome 4 sequence-tagged site STS4-411
Date: 21 Aug 92 10:10:03 GMT
Distribution: bionet
Checksum: 01760     3

LOCUS       HUM4STS411    374 bp ds-DNA             PRI       21-AUG-1992
DEFINITION  Human chromosome 4 sequence-tagged site STS4-411
ACCESSION   L00844
KEYWORDS    STS; human chromosome 4; sequence tagged site.
SOURCE      Homo sapiens, clone p4-352 from a chromosome 4 plasmid library;
            vector pBluescript II KS + (Stratagene)
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 374)
  AUTHORS   Goold,R.D., diSibio,G., Dugaiczyk,A., Smith,K.A., Xu,H.,
            Magrane,G., Lang,D.B., Cox,D.R., Masters,S.B. and Myers,R.M.
  TITLE     Sequence-Tagged Sites for Human Chromosome 4
  JOURNAL   Unpublished (1992) see COMMENT for author address
  STANDARD  full automatic
COMMENT     PCR components: 25 ng of human genomic DNA, 10 pmol of each
            oligonucleotide, 200 micro-M dNTPs, 0.25 U Taq  polymerase (Cetus)
            in 10 micro-l of 50 mM KCl-20 mM  Tris-HCl, pH 8.3 (at room temp),
            2.5 mM MgCl-2.  Initial denaturation at 94degC for 1.5 min, then 30
            cycles of  94degC for 15 sec, 62degC for 23 sec, and 72degC for 30
            sec, followed by a final extension at 72degC for 3.5 min, using a
            Perkin-Elmer 9600 thermocycler. PCR-amplified product size 236 bp.
            Sequence submitted by:
            Human Genome Mapping Center
            Box 0925
            University of California San Francisco
            San Francisco, CA 94143-0925  USA
            Phone: (415) 502-1612   Fax: (415) 476-8391
            e-mail: hgmcprobes@cgl.ucsf.edu
FEATURES             Location/Qualifiers
     misc_feature    33..56
                     /label=Primer1
     misc_feature    complement(245..268)
                     /label=Primer2
BASE COUNT       92 a     73 c     80 g    121 t      8 others
ORIGIN
        1 gatcaacata attcttattt ttacctttgt atgccagtgt atatcgagta aagattattg
       61 tttntgtgag aacatacaga gtaaaaatta tntttttatt acttcttgta tgtttcaggg
      121 acagacgttt gnttcagcct gcccaggtgt gtgacatttt gnagggtgtc attttggtaa
      181 tctgctattt tatgatgcac tatgttgact actccatgat gtaccacctg ataagggggc
      241 agtccgccat caagctctac atcatctaca acatgntgga ggttagggnc ttgcttaccc
      301 cgcacaggcc tggccagggn actcagcttc aagtcttcag ttctgaaggt accncgagag
      361 tcaagaatgc tcta
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Lentinus ponderosus ribosomal RNA (15LR6) large subunit, partial.
Date: 19 Aug 92 10:02:26 GMT
Distribution: bionet
Checksum: 54379     2

LOCUS       LENRRLSV      225 bp ss-rRNA            RNA       19-AUG-1992
DEFINITION  Lentinus ponderosus ribosomal RNA (15LR6) large subunit, partial.
ACCESSION   M98616
KEYWORDS    large subunit ribosomal RNA.
SOURCE      Lentinus ponderosus (library: Duke culture collection 606) cDNA to
            rRNA.
  ORGANISM  Lentinus ponderosus
            Eukaryota; Fungi; Eumycota; Basidiomycotina; Hymenomycetes;
            Agaricales; Tricholomataceae.
REFERENCE   1  (bases 1 to 225)
  AUTHORS   Hibbett,D.S. and Vilgalys,R.
  TITLE     Phylogenetic relationships of the basidiomycete genus Lentinus
            inferred from molecular and morphological characters
  JOURNAL   Unpublished (1992)
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     rRNA            <1..>225
                     /note="Approximately aligns to region between positions
                     845 to 1093in Saccharomyces cerevisiae large subunit
                     nuclear rRNA."
                     /product="large subunit ribosomal RNA"
                     /citation=[1]
BASE COUNT       66 a     45 c     55 g     59 t
ORIGIN
        1 cgtgcaaatc gatcgtcaaa tttgggtata ggggcgaaag actaatcgaa ccatctagta
       61 gctggttcct gccgaagttt ccctcaggat agcagaaact cgtatcagat ttatgtggta
      121 aagcgaatga ttagaggcct tggggttgaa acaaccttaa cctattctca aactttaaat
      181 atgtaagaac gaaggccgtc cttgattgga ccgctcggcg attga
//