From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: E.corrodens ecpA and ecpB genes encoding type 4
Date: 21 Jul 92 10:04:23 GMT
Article-I.D.: genbank.Jul.21.03.04.22.1992.18049
Distribution: bionet
Checksum: 11052     8

LOCUS       EIKECPHAG    3589 bp ds-DNA             BCT       21-JUL-1992
DEFINITION  E.corrodens ecpA and ecpB genes encoding type 4
            N-methylphenylalanine pilin and hag1 gene for hemagglutinin protein
ACCESSION   Z12609
KEYWORDS    hemagglutinin protein; type 4 N-methylphenylalanine pilin.
SOURCE      Eikenella corrodens DNA.
  ORGANISM  Eikenella corrodens
            Prokaryotae; Facultatively anaerobic gram-negative rods.
REFERENCE   1  (bases 1 to 2386)
  AUTHORS   Rao,V.K., Fischlschweiger,W. and Progulske-Fox,A.
  TITLE     Cloning and sequencing of two type 4 N-methylphenylalanine pilin
            genes from Eikenella corrodens
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
REFERENCE   2  (bases 1 to 3589)
  AUTHORS   Progulske-Fox,A.
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
REFERENCE   3  (bases 1833 to 3589)
  AUTHORS   Rao,V.K., Whitlock,J.A. and Progulske-Fox,A.
  TITLE     Cloning, characterization, and sequencing of two hemagglutinin
            genes from Eikenella corrodens
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
COMMENT     *source: strain=ATCC 23834; *source: clone=pVKR 201; *source:
            is_macronuclear=N; *source: is_proviral=N; *source: is_germline=N;
            From EMBL    entry ECECPHAG;  dated 12-JUN-1992.
FEATURES             Location/Qualifiers
     CDS             1098..1577
                     /product="Type 4N-Methylphenylalanine pilin"
                     /gene="ecpA"
                     /codon_start=1
                     /translation="MKQVQKGFTLIELMIVIAIIGILAAIALPLYQDYISKSQVTRAY
                     GEMAGTKTAIEAALFEGRTPVLAATAAAGAAATPPNEWVGMLDNPTSNLLSAATLTPG
                     ANAGDVTFVGTLGENANSSIHGATITLTCTASGEWTCAVAAGTATGWKTKFVPSGCN"
     CDS             1652..2131
                     /product="Type 4N-methylphenylalanine pilin"
                     /gene="ecpB"
                     /codon_start=1
                     /translation="MYKQKGFTLIELMIVIAIIGILAAIALPLYQDHMAKAQINRVFY
                     ELGSTKTAVESILAHGGIPTVDPSQDGVVQNSRRLEFLGLNQNPNSNLIFTASVGLNS
                     NQFERVNATFGRNALPQIQGAVISFVRDNQGQWTCEIDKSNAAYCPPKYTPATCVTI"
     CDS             2248..3150
                     /product="Hemagglutinin protein"
                     /gene="hag1"
                     /codon_start=1
                     /translation="MVSALSCTHERRCYAIRTHLLQNYAGMGLSHYSGSSFVAAQHTG
                     WYLRQAARIARAAEVFELQEDGTVLAEHILQPDSGVWTLYPQAQHKVEPLDDDFAVQL
                     EFHCEKADYFHKKHGMTTTHSAIREAVQTVAPCKTLDLGCGQGHNALFLSLAGYDVRA
                     VDHSPAAVASVLDMAAREQLPLRADAYDINAAALNEDYDFIFATVVFIFLQAGRVPEI
                     IADMQAHTRPGGYNLIVSAMDTADYPCHMPFSFTFKEDELRQYYADWELLKYEEAVGL
                     MHATDAQGRPIQLKFVTMLAKKPG"
BASE COUNT      870 a    799 c    969 g    951 t
ORIGIN
        1 aagcttcagc catatctggg tgcagtttgt gtttcacggc gtggccgggg caggctggca
       61 gcccttggtg cgaccgccgc gtttgggcgg caaccggaaa atgggcgtat ttgccacgcg
      121 cagcccgttc cgccccaatc cgctggggct ctccttgctc aagctggagc gtattgaaac
      181 cgagggtgga gtgcggttat ggtgtggagg ggcggatttg cttgatggca cgccggtgtt
      241 agacatcaag ccctatctgc cttttgtgga agcacagccc gatgccgccc caggcttcgc
      301 cgccgttccg cccgttccgc tcgaagtagc ttgggcagac gagataagcg cagcatccct
      361 gccgccgccc aatcgcctgc tgattgaaca aagcatcgcc caagacccgc gtccggctta
      421 tcaggatacg ccacagcggg tatataagat ggcagtggac gattgggaag tggcgtttcg
      481 gattgaaaaa ggaatggctt taattgaagc agtaatggcg gtggaaggct aaaggctacc
      541 tgaaagctgc ggcatggctt gcgcgtggtt aaagaggagt ctttattaaa ggggcgagtt
      601 gctgcgaagc gaactcttcc ttaatcaaga ggcggcatag caatgccgcc ttaccttatt
      661 tttgcactaa gacagtattg ccacaccgat ggaagtggat agatatagtg gagtgccaaa
      721 aatcgccgct taaaatggca gcaacctaaa ttgccaagcc acgagcccca taaaacggca
      781 atctgtgcaa aatagcgggc caaaaactga cgataaatgt cagccttgca gattagccag
      841 ctatgcagaa tagccatata ggtaggtcgc ttaaatagaa aaataaacaa aaaataattt
      901 aaaaacaaat atttataatg caaataaagc tattttttaa ttttttggca tagccgttgc
      961 tttatgtttc tcgtggcgac ggcaaacaag cctagccgga aatgaagttt tttagttgtc
     1021 tccttctttt ggttgaagtt cccgggtcgg aagattcggt ttatttaagt tccattttta
     1081 acaaggaaaa tgtcacgatg aaacaagtac aaaaaggttt taccctgatc gagttgatga
     1141 ttgttatcgc cattattggt atcttggctg ctatcgccct gccgctgtac caagattaca
     1201 tctctaaatc tcaggtaacc cgtgcatatg gcgaaatggc tggcaccaaa accgctattg
     1261 aagctgctct gtttgaaggt cgtactcctg tattggctgc tactgctgcc gccggcgctg
     1321 ccgctactcc tccgaacgaa tgggtaggca tgctggataa cccgacttct aacctattgt
     1381 ctgctgctac tctgactccg ggcgctaacg ctggtgatgt tacttttgta ggtactcttg
     1441 gtgaaaacgc taatagcagc attcatggtg ctactattac cttgacttgt actgcaagtg
     1501 gtgaatggac ttgtgctgtt gctgctggta ccgcgactgg ctggaaaact aaattcgttc
     1561 ccagcggttg taactaataa ggcttgactg ttagttaaaa acagccacct gagggtggct
     1621 gttttgatat caacaaactg cggtaataaa tatgtataag caaaaaggct ttactttgat
     1681 tgagctgatg attgtgattg ccatcatcgg cattttggct gccattgcat tgccattgta
     1741 tcaagatcat atggctaagg ctcagattaa ccgtgtcttt tatgagttgg gtagtactaa
     1801 gacagcggtg gaatctattt tggcgcatgg cggaattccc actgttgatc cgagccagga
     1861 cggagtggtg caaaatagca gaaggttgga atttttaggt ttgaaccaaa acccgaattc
     1921 caatttaata tttacggcct cagttggatt aaatagcaac caatttgaac gggtcaatgc
     1981 cacctttggc cgcaatgctt tgccacaaat acagggtgca gtaattagct ttgtacggga
     2041 taatcaaggg cagtggactt gcgagataga taaatctaat gctgcatatt gtcctccgaa
     2101 atatacacct gccacttgcg tgacaatttg atttatagaa tattgataat gggagggtca
     2161 tctgtgagag gatggccttt tatattattt tagatagtag taatttacag tgattaattg
     2221 attgttttcc atgcagggta tggaaatatg gtaagtgctc tttcatgtac acatgagagg
     2281 agatgctatg caatccgaac tcatctgcta caaaactatg ccggtatggg attaagccac
     2341 tattccggca gcagttttgt cgcagcacaa cacacaggtt ggtacctacg gcaagctgcg
     2401 cgtattgcgc gggcggctga agtttttgaa ttgcaggaag acggtacggt gctggcggag
     2461 catattttgc agccggattc cggtgtgtgg acgctttatc cacaggctca acacaaggtg
     2521 gagccgttgg atgatgattt tgccgtgcag ctggaatttc attgcgaaaa agcagattat
     2581 ttccacaaaa aacacggcat gactaccacc cattccgcca tccgcgaggc ggtgcaaacc
     2641 gtggcgccgt gtaagacctt ggatttgggc tgtgggcagg ggcacaatgc gctgtttttg
     2701 agcttggccg gatatgatgt gcgcgctgtg gaccacagcc cggcggcagt ggcttcggtg
     2761 ctggatatgg ctgcacgcga acagttgccg ctgcgggcgg atgcttacga tattaacgct
     2821 gctgctttga atgaagatta cgattttatc tttgccacgg tagtgtttat attcctccag
     2881 gcaggccgtg tgccggagat tattgccgat atgcaggcgc atactcggcc tggtggctat
     2941 aacttgatcg tgtcggcaat ggatacagca gactacccct gtcatatgcc gttttcgttc
     3001 acattcaaag aagacgagct gcggcagtat tatgccgact gggaattgtt gaaatacgaa
     3061 gaagccgtgg gcttgatgca cgctactgat gcgcaagggc ggccgattca attgaaattt
     3121 gtgacgatgt tggcgaagaa gcccggctaa actattcgta ttgtatgaaa aaggctgcct
     3181 gaaaactttc aggtagcctt cttttatgtt cgcgcggcaa ctagccggac ttactctttc
     3241 gcaagtatct gcccgcctcc ggctctttcg cattgagaaa accgcctctt tggtgtgccc
     3301 acaggctggc gtagaggccg cccttggcta agagctcggc gtggttgcct tcttccacga
     3361 tgcggccttt atcgagcacg atgaggcgat ccatggcggc ggtggtggag agccggtggg
     3421 cgatggcaat aacggttttg ccttccatca tggtatcgag gctttcttgg atggcggctt
     3481 ccacttcgga atcgagcgcg ctggtggctt catctaaaag cagaatcggt gcgtctttga
     3541 gcatcacttg tgcgataggg atgcgctggc gctggccgcc ggaaagctt
//