From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: E.coli ribonuclease I gene, complete cds.
Date: 29 Dec 92 21:26:13 GMT
Distribution: bionet
Checksum: 00640 4

LOCUS       ECORIBI34    1206 bp ds-DNA             BCT       14-DEC-1992
DEFINITION  E.coli ribonuclease I gene, complete cds.
ACCESSION   M55687 M37486
KEYWORDS    ribonuclease I.
SOURCE      Escherichia coli K12 (strain W3110) DNA.
  ORGANISM  Escherichia coli
            Prokaryotae; Gram-negative facultatively anaerobic rods;
            Enterobacteriaceae.
REFERENCE   1  (bases 1 to 1206)
  AUTHORS   Meador,J.III. and Kennell,D.
  TITLE     Cloning and sequencing the gene encoding Escherichia coli
            ribonuclease I: Exact physical mapping using the genome library
  JOURNAL   Gene 95, 1-7 (1990)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     sig_peptide     250..318
                     /product="ribonuclease I"
                     /gene="rna"
                     /codon_start=1
     mat_peptide     319..1053
                     /EC_number="3.1.27.6"
                     /product="ribonuclease I"
                     /gene="rna"
                     /codon_start=1
     CDS             250..1056
                     /EC_number="3.1.27.6"
                     /gene="rna"
                     /note="on the 100 min. genetic map"
                     /product="ribonuclease I"
                     /codon_start=1
                     /translation="MKAFWRNAALLAVSLLPFSSANALALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKAGY"
BASE COUNT      313 a    296 c    289 g    308 t
ORIGIN      
        1 ggtcctgggg tgattattta cggctgtggc tatgtgaaat caaaagatta ctggcgtctt
       61 ggctgcaatc ttcggggtga tttacatctc tatgttgctg ttggttggct ggccgattct
      121 cgccatgtgg aactaatacg ttcgccaaac gctgaataaa tcatcacgcc cgccaggtaa
      181 gcccacctgg cgggcttttt tatgatttaa tagatagtat tcagtgtatg tatgagttcc
      241 acacccatta tgaaagcatt ctggcgtaac gccgcgttgc tcgcggtttc tctgcttccc
      301 ttctcttctg ccaacgcctt agcgttgcag gcaaaacagt atggcgattt tgatcgctat
      361 gtcctggccc tctcctggca aaccggattt tgccagagtc aacacgatcg aaatcgtaac
      421 gaacgagatg aatgtcgcct gcaaaccgaa acgaccaaca aagctgattt tctgaccgta
      481 catggtctgt ggccaggatt gcctaaatcg gttgctgccc gtggtgttga tgaacgccgc
      541 tggatgcgct tcggttgcgc tactcgccca atcccgaatc taccagaagc gcgcgccagc
      601 cgaatgtgtt catcgccgga aaccggatta tcactggaaa cggccgctaa actaagtgaa
      661 gtcatgccag gagctggcgg acgttcctgc ctggaacgct acgaatatgc caaacacggt
      721 gcctgctttg gttttgatcc ggacgcatac ttcggtacga tggtacgcct gaatcaagaa
      781 attaaagaga gcgaagccgg aaaattcctt gcggataatt acggtaaaac agtgagccgc
      841 cgtgactttg acgccgcctt tgccaaaagc tggggaaaag agaacgtgaa agcagttaag
      901 ctaacgtgcc agggtaaccc tgcgtatttg actgaaattc agatctcgat caaagctgac
      961 gccatcaacg ctccgctttc tgcaaactca ttcttgccac aacctcaccc aggtaactgt
     1021 ggcaaaacct ttgtgattga taaagcgggt tattaatcat ctgatttaca aaacacgcta
     1081 attcgtcttc cataaaccaa ggcgattaag atttatacat aatgcgctgc attgaggaat
     1141 tccctgaatg tgacgcaaat cacttcaaag agtgagcaac agtcagtatc atcatgtgag
     1201 ttaaac
//