From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: IRE1=probable protein kinase required for myo-inositol synthesis
Date: 5 Nov 92 22:59:01 GMT
Distribution: bionet
Checksum: 15436 8

LOCUS       S108302      3961 bp    DNA             NLM       21-OCT-1992
DEFINITION  IRE1=probable protein kinase required for myo-inositol synthesis
            [Saccharomyces cerevisiae, Genomic, 3961 nt]
ACCESSION   S39414 Z11701
KEYWORDS    .
SOURCE      Saccharomyces cerevisiae
  ORGANISM  Saccharomyces cerevisiae
            Unclassified.
REFERENCE   1  (bases 1 to 3961)
  AUTHORS   Nikawa,J. and Yamashita,S.
  TITLE     IRE1 encodes a putative protein kinase containing a
            membrane-spanning domain and is required for inositol phototrophy
            in Saccharomyces cerevisiae.
  JOURNAL   Mol. Microbiol. 6, 1441-1446 (1992)
  STANDARD  full automatic
COMMENT     This entry incorporates information provided by the National
            Library of Medicine's GenInfo Backbone as NCBI gibbsq 108302.
            This sequence comes from Fig. 2.
FEATURES             Location/Qualifiers
     CDS             356..3703
                     /note="Description: probable protein kinase required for
                     myo-inositol synthesis;  For the protein sequence (NCBI
                     gibbsq 108303):  This sequence comes from Fig. 2."
                     /gene="IRE1"
                     /codon_start=1
                     /translation="MRLLRRNMLVLTLLVCVFSSIISCSIPLSSRTSRRQIVEDEVAS
                     TKKLNFNYGVDKNINSPIPAPRTTEGLPNMKLSSYPTPNLLNTADNRRANKKGRRAAN
                     SISVPYLENRSLNELSLSDILIAADVEGGLHAVDRRNGHIIWSIEPENFQPLIEIQEP
                     SRLETYETLIIEPFGDGNIYYFNAHQGLQKLPLSIRQLVSTSPLHLKTNIVVNDSGKI
                     VEDEKVYTGSMRTIMYTINMLNGEIISAFGPGSKNGYFGSQSVDCSPEEKIKLQECEN
                     MIVIGKTIFELGIHSYDGASYNVTYSTWQQNVLDVPLALQNTFSKDGMCIAPFRDKSL
                     LASDLDFRIARWVSPTFPGIIVGLFDVFNDLRTSENILVPHPFNPGDHESISSNKVYL
                     DQTSNLSWFALSSQNFPSLVESAPISRYASSDRWRVSSIFEDETLFKNAIMGVHQIYN
                     NEYDHLYENYEKTNSLDTTHKYPPLMIDSSVDTTDLHQNNEMNSLKEYMSPEDLEAYR
                     KKIHEQISRELDEKNQNSLLLKFGSLVYRIIETGVFLLLFLIFCAILQRFKILPPLYV
                     LLSKIGFMPEKEIPIVESKSLNCPSSSENVTKPFDMKSGKQVVFEGAVNDGSLKSEKD
                     KHDADEDDEKSLDLTTEKKKRKRGSRGGKKGRKSRIANIPNFEQSLKNLVVSEKILGY
                     GSSGTVVFQGSFQGRPVAVKRMLIDFCDIALMEIKLLTESDDHPNVIRYYCSETTDRF
                     LYIALELCNLNLQDLVESKNVSDENLKLQKEYNPISLLRQIASGVAHLHSLKIIHRDL
                     KPQNILVSTSSRFTADQQTGAENLRILISDFGLCKKLDSGQSSFRTNLNNPSGTSGWR
                     APELLEESNNLQCQVETEHSSSRHTVVSSDSFYDPFTKRRLTRSIDIFSMGCVFYYIL
                     SKGKHPFGDKYSRESNIIRGIFSLDEMKCLHDRSLIAEATDLISQMIDHDPLKRPTAM
                     KVLRHPLFWPKSKKLEFLLKVSDRLEIENRDPPSALLMKFDAGSDFVIPSGDWTVKFD
                     KTFMDNLERYRKYHSSKLMDLLRALRNKYHHFMDLPEDIAELMGPVPDGFYDYFTKRF
                     PNLLIGVYMIVKENLSDDQILREFLYS"
BASE COUNT     1328 a    723 c    721 g   1189 t
ORIGIN      
        1 ggagcgtaag cctcttcggg caataccttc gactattcca acaataaaat ttaaaaaaag
       61 agattaatca catagtaaca agaaataaac gaaaaacata tcataggaga tcaatgagcc
      121 aacttcacat aaactaacag tgaaaagtct ataacaatat taattttaca caattaaatc
      181 tacactataa ctggcactgt taataatcgg taatctgcta agtaactttt ttttctcatt
      241 cacaaagcat cgttttcctc ttccccacgt ccattatcac ttttctccat atcacccttc
      301 atacacatta aaaaaacagc atatctgagg aattaatatt ttagcacttt gaaaaatgcg
      361 tctacttcga agaaacatgt tagtattgac actgctcgtt tgtgtgtttt catccatcat
      421 ttcatgctca atcccattgt cgtctcgcac ctcaaggcgg cagatagtgg aagatgaagt
      481 tgcctccact aaaaagctca atttcaacta tggtgtggat aaaaatataa actcgcccat
      541 tcctgctcca agaaccactg aaggtttacc aaatatgaaa ctcagctcat atccaactcc
      601 taacttattg aatactgctg ataatcgacg tgctaacaaa aaaggacgta gggctgccaa
      661 ttctataagt gtaccctatt tggagaatcg ttccttgaac gaactgagtt tatcagatat
      721 actaatcgca gccgacgttg agggtggact tcatgctgta gatagaagaa atggtcatat
      781 catatggtca atcgaaccag aaaattttca acctctgata gaaatacaag aaccttcgag
      841 gttagaaaca tatgaaacgt tgattataga acctttcggt gatgggaaca tttactactt
      901 taacgcccat caagggttac aaaaactgcc tttatccata cgacaacttg tatcaacttc
      961 cccgctgcac ttgaaaacaa atattgtggt taatgactct ggaaaaattg ttgaagatga
     1021 aaaggtctac actggatcga tgagaactat aatgtatact ataaacatgt tgaatggtga
     1081 aattatatca gcgttcggac ctggttcaaa aaacgggtat ttcgggagcc agagtgtgga
     1141 ttgctcacct gaggagaaga taaaacttca ggaatgtgaa aatatgattg taataggcaa
     1201 aactattttt gagctgggaa ttcactctta tgatggagca agctacaatg tcacttactc
     1261 tacatggcag caaaatgttt tagatgttcc cctagcgctt cagaatacat tttcaaagga
     1321 cggcatgtgc atagcgcctt tccgtgataa atcattgcta gcaagcgatt tagattttag
     1381 aattgctaga tgggtttctc cgacattccc cggaattatt gttgggcttt tcgatgtgtt
     1441 taatgatctc cgcaccagtg aaaatatact ggtaccgcat ccctttaatc ctggtgatca
     1501 tgaaagtata tcgagtaaca aagtttactt ggatcagact tcgaacctct cctggtttgc
     1561 attatctagt cagaattttc catctttagt cgaatcagct cccatatcaa gatacgcttc
     1621 cagtgaccgt tggagggtgt cttcaatttt tgaagatgag actttattca agaacgcaat
     1681 catgggtgtt catcagatat ataataatga atatgatcac ctttatgaaa actatgaaaa
     1741 aacgaatagt ttggacacta cgcacaaata tccacctctg atgattgatt cgtccgttga
     1801 tacaaccgat ttacatcaga ataacgagat gaattcacta aaggaataca tgtcaccaga
     1861 agaccttgag gcatatagaa aaaagataca cgagcaaata tcgagagaat tagatgaaaa
     1921 gaaccaaaat tctttgctac tgaagtttgg aagtctagta tatcgaatta tagagactgg
     1981 agtatttctg ttgttatttc tcattttttg tgcaatacta caaagattca aaattttgcc
     2041 gccactatat gtattattat ccaaaattgg atttatgcct gaaaaggaaa tccccatagt
     2101 tgagtcgaaa tcgctaaatt gtccctcttc atcggaaaat gtaaccaagc cattcgatat
     2161 gaaatcaggg aagcaagttg tttttgaagg tgctgtgaac gatggaagtc taaaatctga
     2221 aaaagataag catgatgctg atgaagatga tgaaaaatca ctagatttaa ccacagaaaa
     2281 gaagaagagg aaaagaggtt cgagaggagg caaaaagggc cgaaaatcac gcattgcaaa
     2341 tataccaaac tttgagcaat ctttaaaaaa tttggtagta tccgaaaaaa ttttaggtta
     2401 cggttcatca ggaacagtag tttttcaggg aagttttcaa ggaagacctg ttgcggtaaa
     2461 gagaatgtta attgattttt gtgacatagc tttaatggaa ataaaacttt tgactgaaag
     2521 cgatgatcat cctaacgtca tacgatacta ctgttcagaa acaacagaca gatttttgta
     2581 tattgcttta gagctctgca atttgaacct tcaagatttg gtggagtcta agaatgtatc
     2641 agatgaaaac ctgaaattac agaaagagta taatccaatt tcgttattga gacaaatagc
     2701 gtccggggta gcacatttac attctttaaa gattatccat cgagatttaa agcctcaaaa
     2761 tattctcgtt tctacttcga gtaggtttac tgccgatcag caaacaggag cagaaaatct
     2821 tcgaattttg atatcagact ttggtctttg caaaaaacta gactctggtc agtcttcatt
     2881 tagaacaaat ttgaataacc cttctggcac aagtggttgg agggccccag agctgcttga
     2941 agaatcaaac aatttgcagt gccaagtcga aacggaacac tcttctagta ggcatacagt
     3001 agtttcatct gattcttttt atgatccgtt caccaagagg aggctaacaa gatctattga
     3061 tattttttct atgggatgtg tattctatta tatcctatcc aaagggaagc atccatttgg
     3121 agataaatat tcacgtgaaa gcaatatcat aagaggaata ttcagtcttg atgaaatgaa
     3181 atgtctacat gatagatcct taattgcaga agctacagat ctgatctccc aaatgattga
     3241 tcacgatccg ttaaaaagac ctactgctat gaaagttcta aggcatccgt tgttttggcc
     3301 aaagtcgaaa aaattggagt tccttttaaa agttagtgat aggcttgaaa ttgaaaacag
     3361 agaccctcca agtgccctgt taatgaaatt tgacgccggt tctgactttg taatacccag
     3421 tggagattgg actgtcaagt ttgataaaac attcatggac aaccttgaaa ggtacagaaa
     3481 ataccattca tcaaagttaa tggatctatt aagagcactt aggaataaat atcatcattt
     3541 tatggattta cctgaagata tagcagaact aatggggccg gtacccgatg gattttacga
     3601 ttacttcacc aagcgttttc caaacctatt aataggtgtt tatatgattg tcaaggaaaa
     3661 tttaagtgac gatcaaattt tacgtgaatt tttgtattca taacatgttc atgcccctct
     3721 gcttcttctt ggttgattat tgcattaatg ttactttgat cattagtagt aaccatccat
     3781 aatttttacg cccttgcata aatgaaaaaa agttgtactt cttcatgaac ctatactact
     3841 tttctcatcc ctacataaac tacccgacag acatttaatt ttataaatac atatctcata
     3901 aacatcgaac atcgacatac tactccagtt attcaacact gacttttttg gtggaaagct
     3961 t
//