HDTenEightyP/NetNews huggingface dataset:
From: doshay@ursa.arc.nasa.gov (David Doshay)
Newsgroups: bionet.neuroscience
Subject: Re: re:repolarization time of neuron
Date: 7 Apr 92 18:12:49 GMT
Article-I.D.: news.1992Apr7.181249.27724
Distribution: bionet
Organization: NASA-Ames Biocomputation Center

In article <9204062228.AA17922@aragorn.stanford.edu>, mbieda@ARAGORN.STANFORD.EDU (Mark Bieda) writes:
|> 
|> 4) If only we could model a single neuron realistically....

But we are getting very close. That is just what I am working on.

David		doshay@ursa.arc.nasa.gov
Biocomputation Center
NASA Ames Research Center
From: MJG@A1.PSUPEN.PSU.EDU ("Mark J. Guiltinan 814-863-7958")
Newsgroups: bionet.genome.arabidopsis
Subject: Tobacco Fertilizer
Date: 8 Apr 92 16:09:00 GMT
Article-I.D.: PSUPEN.01GILPW3GGN88X0KF9
Distribution: bionet


To the helpful person at NC State who responded to my request for optimal 
tobacco growth conditions.  After I sent a print command of you letter I 
deleted it from my computer only to later find out that after a paper jam I had 
no copies of it intact.  Could you please reseend.  Also, do you have any 
experience with lighting conditions etc. for speeding up flowering or 
optimizing growth.  Thanks again.

Mark Guiltinan
Penn State Biotech. Institute
From: 21847CRS@MSU.EDU ("Chris.Somerville")
Newsgroups: bionet.genome.arabidopsis
Subject: Runoff Election
Date: 10 Apr 92 00:29:00 GMT
Distribution: bionet

Please vote for one of the following nominees for membership on the North
American Arabidopsis Steering Committee.

Mark Estelle
Howard Goodman
Dave Meinke

Please send your vote to biovote@genbank.bio.net

Please do not use the "REPLY" function on your e-mail system.

Thanks, Chris
From: BTNYDWM@OSUCC.BITNET
Newsgroups: bionet.genome.arabidopsis
Subject: Position at Noble Foundation
Date: 8 Apr 92 16:04:00 GMT
Distribution: bionet

Position Available - Section Head - Plant Molecular Genetics

     The Samuel Roberts Noble Foundation is seeking applicants
with the necessary qualifications to initiate a new research section
within the Plant Biology Division.  Candidates should have a
proven ability in applying their expertise to genetically tractable
systems such as (but not limited to) Arabidopsis.  The successful
candidate will be expected to develop a vigorous research program
utilizing genetic methods for elucidating the molecular mechanisms
underlying plant responses to biotic and abiotic stress, or for
studying topical problems in plant development.

     The position is one of four Section Head position in the Plant
Biology Division.  The successful applicant would be expected to
interact with the other groups which work on the biochemistry and
molecular biology of plant defense response genes, molecular virology,
and crop protection through gene transfer technology.  The Foundation
offers a unique environment for creative and productive research with
stable in-house funding and state-of-the-art equipment and facilities
including a core-facility for DNA synthesis, automated DNA
sequencing and peptide sequencing.  Excellent prospects exist for
achievement-based development of the Plant Genetics Section.
Technical and postdoctoral positions within the Section will be
advertised on appointment of the Section Head.

     The Plant Biology Division has formal collaborative ties with
the laboratories of Drs. Christopher Lamb and Joanne Chory (Salk
Institute, San Diego) and contributes to a National Science Foundation
EPSCoR research cluster with researchers at Oklahoma State
University (including Drs. David Meinke and John Sherwood) in the
area of Molecular Analysis of Plant Biotic Stress.  Currently the
Division employs 9 permanent faculty, 20 postdoctoral fellows and
13 support staff.

     To apply, submit curriculum vitae, statement of research interests
and names of three referees to:

Dr. Richard A. Dixon
Director, Plant Biology Division
The Samuel Roberts Noble Foundation, Inc.
P.O. Box 2180, Ardmore, OK  73402  USA
Phone (405)-223-5810;  FAX (405)-221-7380

The Samuel Roberts Noble Foundation is an Equal Opportunity Employer.
From: benfey@ROCKVAX.ROCKEFELLER.EDU
Newsgroups: bionet.genome.arabidopsis
Subject: protein sequence homology
Date: 8 Apr 92 17:03:56 GMT
Distribution: bionet

We have a deduced protein sequence for which we have not yet found any
significant homology.  Does anyone know of someone who is good at looking
at protein sequence and determining structural (e.g. membrane spanning
segments) or functional (e.g. kinase domains) that wouldn't show up in
a simple homology search?  Also, by now there must be a number of 
sequenced plant genes that have no homology and have not yet been 
published.  Is there any interest in finding a central neutral 
repository for such so that we can all compare with each other and
see if some interesting things show up?  If there is interest I would be happy to 
to try to organize this.

Philip Benfey
Newsgroups: bionet.genome.arabidopsis
From: kerrylee@athena.mit.edu (Kerry Lee Tucker)
Subject: cDNA 
Organization: Massachusetts Institute of Technology
Date: Fri, 10 Apr 1992 20:51:51 GMT

Hello, people.  I apologize if this is not the proper forum to air this
request, but I could not find  another A. thaliana network so here goes:

I am looking for any and all cDNA libraries made from Arabidopsis thaliana
tissue.  Specific tissues would be of particular interest, but anything is better
than not.

Please reply to me at the following address:

kerrylee@athena.mit.edu  (617) 253-4723
 Thank you.
Newsgroups: bionet.genome.arabidopsis
From: samodena@csemail.cropsci.ncsu.edu (S. A. Modena)
Subject: Re: Fertilizers for Tobacco
Organization: Dept. of Crop Science, NCSU, Raleigh, NC.
Distribution: bionet
Date: Mon, 6 Apr 1992 22:52:41 GMT

In article <01GIJ1C5ELP08X0M0A@PSUPEN.PSU.EDU> MJG@A1.PSUPEN.PSU.EDU ("Mark J. Guiltinan 814-863-7958") writes:
>
>     Has anyone out there determined the optimal fertilizer conditions for the 
>growth of tobacco plants?  I know they like high N but how high?  Any other 
>gems of wisdom would be much appreciated.
>
>     Mark Guiltinan
>     Penn State Biotechnology Institute
>     mjg@psupen.psu.pen
>

1 Liter of Solution A contains:  1 mL of 1M KH2PO4      
                                 5 mL of 1M KNO3
                                 5 mL of 1M Ca(NO3)2
                                 2 mL of 1M MgSO4

1 Liter of Solution B conatins:  2.86 grams of H3BO3
                                 1.81 grams of MnCl2.4H2O
                                 0.22 gram  of ZnSO4.7H2O
                                 0.08 gram  of CuSO4.5H2O
                                 0.09 gram  of H2MoO4.H2O

1 Liter of Solution C contains:  7.45 grams of Na2EDTA
                                 5.57 grams of FeSO4.7H20

Mix 1 Liter of Solution A + 1 mL Solution B + 1 mL Solution C; and
add water to make up 2 Liters.

This is half-strength Hoagland solution and plants can be watered
with it daily.  Best to water from the bottom: set pot in shallow tray
of nutrient solution until surface of potting material is moist.

This is how the Wernsman Lab fertilize their greenhouse and growth
chamber plants.  (Tobacco is a way-of-life in this state.)
---
+------------------------------------------------------------------+
|     In person:  Steve Modena     AB4EL                           |
|     On phone:   (919) 515-5328                                   |
|     At e-mail:  nmodena@unity.ncsu.edu                           | 
|                 samodena@csemail.cropsci.ncsu.edu                |
|                 [ either email address is read each day ]        |
|     By snail:   Crop Sci Dept, Box 7620, NCSU, Raleigh, NC 27695 |
+------------------------------------------------------------------+
         Lighten UP!  It's just a computer doing that to you.
OOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOO
From: krdavis@magnus.acs.ohio-state.edu (Keith R Davis)
Newsgroups: bionet.genome.arabidopsis
Subject: Arabidopsis library request
Date: 10 Apr 92 17:46:51 GMT
Organization: The Ohio State University

Arabidopsis Researchers:

The ABRC at Ohio State University is ready to start receiving materials for the
DNA stock center.  We would like to start first with libraries.  Our current
policies for receiving libraries are:

1) we will not distribute libraries that have been amplified more than twice.

2) we ask that donators of once-amplified libraries try to provide sufficient
quantities of the library for direct distribution by the Center.  In cases
where this is not possible, we will amplify the library, as long as this does 
not increase the total number of amplifications beyond two.

If you are willing to send us your library, please use the following donation
form.  It is important that as much information be provided as possible.  Also,
please send the completed form or contact us by other means before sending the
library.  This  will ensure that we will be anticipating its arrival and can
follow up on any apparent mail delays in a timely fashion.

Thanks in advance for your help in establishing the stock center.

Keith Davis
DNA Stock Center
Davis.68@osu.edu

ABRC Library Submission Form

Library name

Date generated

Vector type

     phage
     cosmid
     plasmid
     YAC
     other

Type of library

     cDNA
          random primed
          oligo dt
          other

     genomic

          Restriction sites used to generate DNA fragments



Tissue source
     whole plant
     leaf
     root
     other

Treatments plants or tissues received prior to library construction




Details of library construction





Host strain

Antibiotic selection

Restriction map of vector and cloning sites used





Average insert size

Library submitted by

Date

References
From: MJG@A1.PSUPEN.PSU.EDU ("Mark J. Guiltinan 814-863-7958")
Newsgroups: bionet.genome.arabidopsis
Subject: Fertilizers for Tobacco
Date: 6 Apr 92 18:10:00 GMT
Distribution: bionet


     Has anyone out there determined the optimal fertilizer conditions for the 
growth of tobacco plants?  I know they like high N but how high?  Any other 
gems of wisdom would be much appreciated.

     Mark Guiltinan
     Penn State Biotechnology Institute
     mjg@psupen.psu.pen
From: nigel@codon3.berkeley.edu ()
Newsgroups: bionet.genome.arabidopsis
Subject: Splice site consensus for A.T (?)
Date: 14 Apr 1992 16:12:16 GMT
Organization: University of California, Berkeley
Reply-To: nigel@codon3.berkeley.edu ()
NNTP-Posting-Host: codon3.berkeley.edu

>From: sam@weeds.mgh.harvard.edu (Sam Cartinhour)
>Subject: splice sites in arabidopsis

The following information was obtained for Arabidopsis by asking AATDB for the
"splice concensus", based on 123 introns:

     5' concensus          --------intron---------->

A    34    47    80    12  |   0    1    73    68    31    37    42
C    33    42    15     1  |   1    0     8    15    10    20    16
G    30    21     9   101  | 119    3    15     8    58    10    20
T    26    13    19     9  |   3  119    27    32    24    56    45
X     0     0     0     0  |   0    0     0     0     0     0     0


     3' concensus  ----------intron--------->

A    22    22    26    46     7   118     2  |   30    35    31    33
C    17    18     9     9    74     0     1  |   18    20    22    20
G    21    25     8    42     2     3   118  |   62    18    23    39
T    63    58    80    26    40     2     2  |   13    50    47    31
X     0     0     0     0     0     0     0  |    0     0     0     0

Sam Cartinhour
cartinhour@frodo.mgh.harvard.edu


************************************************************************

Don't pester Sam about the DB just yet:

>Subject: The weed DB
>Status: R


Hi Nigel

Mike Cherry and I are the developers of AATDB. Right now the database
is in "beta" test, meaning that we have distributed it to a handful of
sites so that they can bang away at it. The major purpose of this
phase is to catch data errors, inconsistencies, and omissions.  The
software itself has already been tested extensively (it has been in
use for nine months as the program that runs the worm database,
ACEDB). 

In one or two weeks we are going to open up distribution to basically
any site that is "unix-ready", i.e., has the appropriate hardware and
can set up and run the database right away. We will be making an
announcement about it to our aatdb-info list. Again, the focus will be
on getting user feedback on data quality, but also we will want to
begin setting up a system whereby users can contribute data if they
want.

sam

*************************************************************

-nigel


                                [>======<]   
       Nigel Walker              \  Mu  /   nigel@enzyme.berkeley.edu   
       Dept. of Plant Biology     \    /          (510) 642-7085  Lab
       U.C Berkeley                \  /           (510) 642-4995  Fax
       Berkeley, CA 94720           \/            (510) 654-LEAF  Home
                              ==============
From: 21847CRS@MSU.EDU ("Chris.Somerville")
Newsgroups: bionet.genome.arabidopsis
Subject: More Runoff Election
Date: 10 Apr 92 00:33:00 GMT
Distribution: bionet

I forgot to add that the runoff election will close April 15.

Thanks, Chris
From: 21847CRS@MSU.EDU ("Chris.Somerville")
Newsgroups: bionet.genome.arabidopsis
Subject: Election results
Date: 10 Apr 92 00:25:00 GMT
Distribution: bionet

Five nominees received a clear majority, three others were tied for the 6th
position.  Therefore, we need to have a "runnoff" for this position which will
be announced in a separate notice.

The five elected members are Fred Ausubel, Joanne Chory, Joe Ecker, Elliot
Meyerowitz, Chris Somerville

The three tied nominees are Mark Estelle, Howard Goodman, Dave Meinke

My thanks to all those who agreed to let their name stand, to the large number
of people who voted, and particularly to Dave Kristofferson who made it work.
I think this is a useful step forward in generating a truly representative
"voice" for the community.  I hope that when you have an idea about something
that should or could be done by a quasi organized group, you will contact one
of the members of the committee and inform them.

Chris Somerville, for the NA Arabidopsis Committee
From: nigel@codon1.berkeley.edu
Newsgroups: bionet.genome.arabidopsis
Subject: Intron/Exon consensus sequences?
Keywords: Splice sites, consensus, plant
Date: 11 Apr 92 04:13:47 GMT
Reply-To: nigel@codon1.berkeley.edu ()
Organization: University of California, Berkeley
NNTP-Posting-Host: codon1.berkeley.edu

Hi folks,

Can (will) anyone out there point me in the direction of the consensus
splice sites for plant introns?

To be honest with you I'm working with a (dare I say it) maize gene, but
as far as I can tell the maize folks don't have a forum such as this.

muchadvancethankance

-nigel


                                [>======<]   
       Nigel Walker              \  Mu  /   nigel@enzyme.berkeley.edu   
       Dept. of Plant Biology     \    /          (510) 642-7085  Lab
       U.C Berkeley                \  /           (510) 642-4995  Fax
       Berkeley, CA 94720           \/            (510) 654-LEAF  Home
                              ==============
From: PAMK@CC.USU.EDU
Newsgroups: bionet.genome.arabidopsis
Subject: seed size
Date: 7 Apr 92 20:29:00 GMT
Distribution: bionet

In response to Fred Lehle's query about seed size variation among
Arabidopsis genotypes, I would like to point out a recent paper on 
the effects of genotypic differences in seed size on seedling survival:

Krannitz, Aarssen and Dow (1991) The effect of genetically based 
differences in seed size on seedling survival in Arabidopsis
thaliana (Brassicaceae).  American Journal of Botany 78: 446-450.

Pam Krannitz
From: K445711@CZHRZU1A.BITNET (Robert Dudler)
Newsgroups: bionet.genome.arabidopsis
Subject: suspension cultures
Date: 14 Apr 92 16:01:50 GMT
Article-I.D.: genbank.9204141422.AA06831
Distribution: bionet


Dear Networkers,  could anyone give me a tip on how best to establish cell susp
ension cultures of Arabidopsis, particularly RLD. I've got the paper by Gleddie
 (Plant Cell Reports 8:1-5 1989) but wondered if there was any significant impr
ovements or important further practical hints available. Please reply to Robert
 Dudler over the network or K445710@CZHRZU1A.BITNET
                   Thanks in advance
Newsgroups: bionet.genome.arabidopsis
From: Beverley.Green@MTSG.UBC.CA
Newsgroups: bionet.genome.arabidopsis
Subject: Post-doctoral Position
Date: 7 Apr 92 20:16:40 GMT
Article-I.D.: mtsg.3689980
Distribution: bionet


                        POSTDOCTORAL POSITION
     CHARACTERIZATION OF HIGH CHLOROPHYLL FLUORESCENCE MUTANTS OF
                             ARABIDOPSIS.
 
      We have isolated a number of high chlorophyll fluorescence
(hcf) mutants of Arabidopsis blocked in photosynthetic electron
transport. Two of them are particularly interesting in that they
appear to affect the processing or stability of some polycistronic
chloroplast transcripts in a selective way (Dinkins et al,ISPMB
abst.#1969). These EMS-induced mutants need to be further
characterized molecularly to determine the exact nature of the defect.
We will also be screening T-DNA mutagenized lines for similar hcf
mutants.
      A position is available immediately for a postdoctoral fellow
to work on the characterization of these mutants and participate in
the isolation of T-DNA-induced hcf mutants. The most suitable
candidate for this position would have had some practical experience
in plant genetics (not necessarily Arabidopsis) as well as basic
molecular biology and biochemical techniques. Photosynthesis aspects
can be learned on the job; in fact, this would be a good opportunity
for a plant molecular biologist/geneticist to diversify into a
fascinating area.
      I would like to fill this position by July 1 if possible. If
this project sounds interesting to you, please call me at 604-822-2349
or send a FAX indicating your general background and interests (604-
822-6089).
      Dr. Beverley R. Green,
      Botany Department,
      University of B.C.,
      Vancouver, B.C. V6T 1Z4
      Canada.
USERBRG@UBCMTSG  (Bitnet)    or USERBRG@mtsg.ubc.ca   (Internet)
From: robert.h.gross@dartmouth.edu (Robert H. Gross)
Newsgroups: bionet.biology.computational
Subject: neural nets and protein structure prediction
Date: 14 Apr 92 18:45:10 GMT
Distribution: bionet
Organization: Dartmouth College, Hanover, NH
Originator: biocomp@lerkim.umiacs.umd.edu

Hi folks,

I am interested in identifying some recent papers that address the use
of neural nets in determining protein structure. Can someone please
post some references?

Thanks.

Bob Gross
Dartmouth College
bob.gross@dartmouth.edu
From: lambert@hydrogen.oscs.montana.edu (Chris Lambert)
Newsgroups: bionet.biology.computational
Subject: Re: neural nets and protein structure prediction
Date: 15 Apr 92 13:04:55 GMT
Distribution: bionet
Organization: Montana State University
Originator: biocomp@lerkim.umiacs.umd.edu


In article <1992Apr14.184510.9403@dartvax.dartmouth.edu> robert.h.gross@dartmouth.edu (Robert H. Gross) writes:
>Hi folks,
>
>I am interested in identifying some recent papers that address the use
>of neural nets in determining protein structure. Can someone please
>post some references?
>
>Thanks.
>
>Bob Gross
>Dartmouth College
>bob.gross@dartmouth.edu

There are more recent articles but this reference was hanging
around:

Qian, N. & Sejnowski, T. J., Predicting the Secondary Structure of
globular Proteins Using Neural Network Models.  (1988) J. Mol. Biol.
202, 865-884.


Chris Lambert
Montana State University
108 Gaines Hall
Bozeman, MT. 59717
(406) 994-1964
lambert@hydrogen.oscs.montana.edu
From: EMESAROS@YUBGEF51.BITNET ("GABOR MESARO[")
Newsgroups: bionet.journals.contents
Subject: register
Date: 7 Apr 92 23:22:00 GMT
Distribution: bionet


register Gabor Mesaros
subscribe bio-jrnl
From: CFARMER1@UA1VM.BITNET ("Chet Farmer, Asst. Dir., Project IDEALS")
Newsgroups: bionet.journals.contents
Subject: ANNOUNCEMENT OF SAGSET CONF
Date: 9 Apr 92 22:23:15 GMT
Distribution: bionet


  * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * *
* *                                                                 * *
*     ----------------------                                          *
*     Call for participation:  ---------------------                  *
*     ----------------------   Joint 1992 conference:  ///////////    *
*                              ---------------------   S A G S E T    *
*                                                       I S A G A     *
*   Society for the Advancement of Games and           \\\\\\\\\\\    *
*   Simulations in Education and Training                             *
*                   International Simulation and Gaming Association   *
*                                                                     *
*   Conference theme:        Developing transferable skills through   *
*   ----------------                          simulation and gaming   *
*                                                                     *
*   18-21 August, 1992       Napier University, Edinburgh, Scotland   *
*                                                                     *
*   For further information:                          Fred Percival   *
*   =======================       SAGSET/ISAGA Conference Secretary   *
*                                                 Napier University   *
*                                                 219 Colinton Road   *
*   Telephone:  44 / 31-455-4394                 Edinburgh EH14 1DJ   *
*   Facsimile:  44 / 31-455-7989                           Scotland   *
*                                                                     *
* *                                                                 * *
  * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * *

+-----------------------------------------------------------------------+
|  Chet Farmer, Assistant Director   |   English / 103 Morgan           |
|  Project IDEALS -- FIPSE, DoE      |   Tuscaloosa, AL  35487-0244     |
|  University of Alabama             |   tel 205-348-9494               |
+-----------------------------------------------------------------------+
From: MPB@CBR.DWE.CSIRO.AU (Mark Bradley)
Newsgroups: bionet.molbio.methds-reagnts
Subject: RE: A quick help is needed from Ohio with Western blotting!
Date: 16 Apr 92 18:44:53 GMT
Article-I.D.: cbr.920416134453.1fa3
Distribution: bionet

Yes I think you will get diffusion overnite. Why dont you change
your blotting system (and gels) to a mini system, where each minigel
takes only 30-60 min to transfer. Also the biorad miniblot tank 
takes 2 gels at once. I guess this wont help your present problem though.
Mark Bradley