From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: D. virilis glycerol-3-phosphate (G-3-P) dehydrogenase gene,
Date: 12 Jan 93 05:46:03 GMT
Distribution: bionet
Checksum: 16799 13

LOCUS       DROGPDH      7393 bp ds-DNA             INV       12-JAN-1993
DEFINITION  D. virilis glycerol-3-phosphate (G-3-P) dehydrogenase gene,
            complete cds.
ACCESSION   D10697 D01107
KEYWORDS    G-3-P dehydrogenase; flight muscle metabolism.
SOURCE      Drosophila virilis (individual_isolate alphaGPDHM, strain wild)
            adult DNA.
  ORGANISM  Drosophila virilis
            Eukaryota; Animalia; Metazoa; Arthropoda; Uniramia; Insecta;
            Pterygota; Neoptera; Holometabola; Diptera; Brachycera;
            Cyclorrhapha; Schizophora; Drosophiloidea; Drosophilidae.
REFERENCE   1  (bases 1 to 7393)
  AUTHORS   Tominaga,H., Shiba,T. and Narise,S.
  TITLE     Structure of drosophila virilis glycerol-3-phosphate dehydrogenase
            gene and a comparison with the drosophila melanogaster gene
  JOURNAL   Biochim. Biophys. Acta 1131, 233-238 (1992)
  STANDARD  full automatic
COMMENT     Submitted (17-JUL-1992) to DDBJ by: Sumiko Narise Biological Lab.
            Faculty of Science, Josai University 1-1 Keyakidai, Sakado Saitama
            350-02 Japan Phone:  0492-71-7965 Fax:    0492-71-7985.
FEATURES             Location/Qualifiers
     CAAT_signal     1065..1070
     TATA_signal     1178..1183
     prim_transcript 1210..6946
                     /note="alphaGPDH mRNA and introns"
     exon            1348..1391
                     /number=1
     intron          1392..1473
                     /number=1
     exon            1474..1651
                     /number=2
     intron          1652..4307
                     /number=2
     exon            4308..4514
                     /number=3
     intron          4515..4584
                     /number=3
     exon            4585..4957
                     /number=4
     intron          4958..5024
                     /number=4
     exon            5025..5178
                     /number=5
     intron          5179..5245
                     /number=5
     exon            5246..5342
                     /number=6
     intron          5343..5956
                     /number=6
     polyA_signal    5671..5676
     exon            5957..5990
                     /number=7
     intron          5991..6581
                     /number=7
     polyA_signal    6294..6299
     exon            6582..6594
                     /number=8
     polyA_signal    6938..6943
     polyA_signal    7035..7040
     polyA_signal    7047..7052
     polyA_signal    7062..7067
     polyA_signal    7185..7190
     CDS             join(1348..1391,1474..1651,4308..4514,4585..4957,
                     5025..5178,5246..5342)
                     /EC_number="1.1.1.8"
                     /gene="alphaGPDH"
                     /product="G-3-P dehydrogenase"
                     /codon_start=1
                     /translation="MAEKVNVCIVGSGNWGSAIAKIVGANAAALPEFEERVTMFVYEE
                     MIDGKKLTEIINETHENVKYLKGHKLPTNVVAVPDLVEAAKNADILIFVVPHQFIPNF
                     CKQLLGKIKPNAIAISLIKGFDKAEGGGIDLISHIITRHLKIPCAVLMGANLANEVAE
                     GNFCETTIGCTDKKYGKVLRDLFQANHFRVVVVEDADAVEVCGALKNIVACGAGFVDG
                     LKLGDNTKAAVIRLGLMEMIRFVDVFYPGSKLSTFFESCGVADLITTCYGGRNRRVSE
                     AFVTSGKTIEDLEKEMLNGQKLQGPPTAEEVNYMLKNKGLEDKFPLFTAIHKICTNQL
                     KPKDLIDCIRNHPEHM"
BASE COUNT     2490 a   1318 c   1245 g   2340 t
ORIGIN      
        1 aactcagcct cgtgtttgtt ttttctaact atgcgtcaat gcgcattcga aaagggctgt
       61 tcaggggatg gttgttagaa ccaacgatgt cgttatggcc ttaatagatt aacgcattta
      121 tttataagca acagtgagtg tcaagtatat gtagacgagt ggcgacaaca gctaagcttg
      181 agagagaatc atgagctttg agatttacat acacttcagc ttcaaagctt caaatccact
      241 taaatattta ttttattatc actatttgac tttagtttaa cgtaaatgga aagctatgta
      301 agctgatatt gaaaactgat cgcagacaat ccgccgtttg tcatatgtca ccaaggagca
      361 gctaacttac ttctgttccc ttctgttcgt cttcgtgtcc acatatgttt gatattacgt
      421 ttatttataa tggtaaaaat ttttcatttt tatcaacttt atggcagtaa ataatttgtt
      481 aagttgtgta acatgtgacc aaaaccgtaa aacacatacg taagcaaatg tcaaaaatgt
      541 taataagcaa atttgaattt cgcgcgcagt taacagtggg ctaacagcat gtaactcaac
      601 agttcatttg agcataaggg agtctggcaa atagccataa aaaattatga tttaaatata
      661 ttcaaactta tttctatttt cggaattgaa aaaaactaat ttgcacatta catttttaaa
      721 tttattaaaa tggcatcaat tgcaacgaaa aaaaaacgat agcaaatcag ctgttcaatg
      781 taaaatcaaa acaaaacata aacaatggaa atctatgctc ataaataagg caaattcagc
      841 agaaatctct tggagaatat aatttttaaa tattctggcg ggtttttctt agaaatttac
      901 ttttcttcca aaacaattgt taaacgtaaa tagctgttcg cgccatcttt caaaccaaat
      961 agaatttata taaaatcagt gcaaatttta tatgatttca taataagctt atgattcgct
     1021 tatttccgaa tcaaaactaa acttaaaata tttatttgaa atatgccaat tgatgcgtaa
     1081 cattcgattt ctcgatacac actatcgcat ggcaatcaat aatgttatcg atatgtgtat
     1141 cgtttcgtac acttgcaaat acgcgtttac tgaatagtat ttattaaatg tgccgtggtt
     1201 tcgacgtgtc agttcgtagt aaataattcg ctgtgctagt cgcatatacc aagagtctga
     1261 cacgcgtaca aatcaatcga cagagttgaa aacactttgc catacaacaa cagcaacaac
     1321 gaaaacaaca acaattaata cagcagcatg gcggaaaaag taaatgtttg catcgtgggc
     1381 tctggcaact ggtgagtgca ttaagagcat taataatgtc attagttata taataattgc
     1441 atattgcacc ttggcatttt gaacctggtg caggggttcg gccatagcga aaatcgtggg
     1501 cgccaatgcg gccgcgctgc ccgagtttga ggagcgcgtc acaatgtttg tctacgagga
     1561 gatgattgat ggcaagaagt taacggaaat tatcaatgaa acgcacgaga atgttaaata
     1621 cctgaaggga cacaaactgc caacaaatgt ggtgagtaca aaaaagcgca atgtcatgca
     1681 taactgtggc ggctaactgt taattaaaat catcaacata aagaaaaagg tgtgaaaaca
     1741 aaaaaaaaac aaaaaaacaa accgccgccg cttctgttat gcaatattcg tattagtttt
     1801 agtgcaatta ttaacaagtg cagcgacggc tcgtgcccct tacataaaag acgccgctgc
     1861 cttgatcttg gccatcgtat ttacgtcaaa ttatctaatc agacgaatgc gcggacggac
     1921 atactgacgg gacatgcaca tacagctatt tttacaactc acctcgcgca ctcacacact
     1981 ctctcgctct cacacactct tggccaacgc tgcacttttt gttttttttt attttttggt
     2041 cagggccaaa aacaacaaca acaaaaagtt tcgccttgct ctgacgtctg cacacaaacg
     2101 aaaccttctg ccaagctggc agtgacagtt ctgccgctgt gtctttgacg taggtcaagg
     2161 ccggcttgac gtgtgcgcgc ctcgcactct cttatgcatt ctctcttaat tgctgtgcct
     2221 aagagaaaca tatgtacagc aattgtgaca gttagacaga tcaatgtttt gccataatac
     2281 atttatacgg aaatatttca catgcataac ataacaattt gcacagctga actgataaga
     2341 gcactgttga ctgtttgtat tcgcttgatt atagataacc cgctttgtta gcgggtcatg
     2401 ttaaacacgc tattttagtt gtttcgataa agtgccgact aataaatatg taatggcctg
     2461 tactacatat cgattcggtt tgaaaggtaa caatacgttt tgcggctctt cagttcgtat
     2521 atattgccaa ttacaactaa ctctgactat tgacaacgtc tagatttgtt cggataaaat
     2581 atctttattt atgaattaaa tggaaatttt ccattagagc taatttatca gtagaatttg
     2641 taggatttcg actagacagc tgtcactgca gcattgataa aagagaaaaa caaattaaat
     2701 cgtttctatg tctgttccta ttgtgtttct atatatttgt atgccagcgc gactatatat
     2761 atatatatat acatgtatac cttaatttat atagctatgc tttttggcaa actttcaata
     2821 gtgaaagcaa aaacaaaaac tgattttttt attaacaatg tcaaaattgc aggcaaatat
     2881 gcaaagcatt tctcaaatca aaagttaaac gttaaattaa gggaaatagt agaaaatcaa
     2941 gtaaaatatt gtagcggaaa atgattgtgt tttctgataa caatttgttt gttttctcat
     3001 ctatttttat gccccaatac ttttgacatg aaatgtgtca ggcattgaag aaagcatgtt
     3061 ctagcttata acaatatata tatatatata tatatatgga atgataattt gggcaaatat
     3121 aaatttaaat agaaatttta attccgcatc aaaatacaat atttttctca tataaattca
     3181 caaatattta gtctatctag tctagtctat taattaattt gcttaatatg gttgaaaaac
     3241 agttaacatt ataattatgc acatgattag agatgggtta agcaattatt tatttgactt
     3301 taaacgcaat tagggttttg cacagttgat tatcaacaac aagtgcaaat gctgaacttt
     3361 ttacaggcaa acgaattgat gaaatgcatt tggttccaac ctattaaact ttgtgcttgt
     3421 cgctttagtt atggaaaatg tttttgcaat aacaaaaaaa aagcaaatat tgcctgattt
     3481 atgccctaaa attgggtata atgttacggt gcaaatgtat gtaacaggcg aatagaatat
     3541 atatatatat atttgatggg gaaacaagca gagccataat acaaaggcta aaaacaagcc
     3601 taactcggca gctataaaag ataagacata ctatatttga tatttaatct cacgtacaca
     3661 aacaagaaat atgcaacatg ttgtatattg ttgacatatt taagtactga aaatctcgtt
     3721 aggatctggc tataaacagc gaagttatta aagaacgcag ttttctatat tatatacaac
     3781 atatgttcgc tagacttggg ctttactcta tacattgtta aaagtaatta gctttctttt
     3841 tagattgttt aatatgcatt aattttaaag tccaaatatt agcagagttt tttggtcaaa
     3901 aacttgtttt cttatctaca gcgaatataa atcgcaaaaa aaaagacaca atatgcgaga
     3961 tagatatctc gcttgaactt taaaccataa gtgtaagcct tataaacagg tttccaacac
     4021 aagccgatgg ctaattaatt caaatgagcg ttagagagag agagacaagc ctctaaatat
     4081 ggaaaacacg tgcatttcaa gtgattttcg tagagatttc aaagtttcct tcattcaacc
     4141 ttgagctctg tgtttggttt gttctattcg ccgcttatca gagtaactat ggccgcttat
     4201 cggaacattc gcttgtcgtt tttattattg atgcacacac acacacacac acacaaacac
     4261 ctttcgcatt taatttgtta aagtggtctc cctctctctc tttataggtt gctgtacctg
     4321 atctggttga ggctgccaag aatgccgata tactaatctt tgtggtgcca catcaattta
     4381 taccaaattt ctgcaagcaa ttgctgggca agatcaagcc aaatgccatt gccatatccc
     4441 tgatcaaggg cttcgataag gccgagggcg gcggcattga tctgatatcg cacattatta
     4501 cccgccattt gaaggtaagc cccaaattga gaagatgttc ttatgcaatc gttagctaat
     4561 gttgattcta ttatctacaa tcagatacca tgcgccgtgc tgatgggcgc taatctggcc
     4621 aacgaggtgg ccgagggcaa cttttgcgag acgacaatcg gctgcacgga caaaaagtac
     4681 ggcaaggtgc tgcgtgatct cttccaggca aatcattttc gtgtcgtggt cgttgaggat
     4741 gccgatgccg ttgaggtttg cggcgccctg aagaacattg tggcctgtgg tgcgggcttt
     4801 gtcgatggtc tcaagctggg cgataatact aaggcagcgg tcattcgttt gggtctcatg
     4861 gaaatgatac gctttgtcga tgtcttctat ccgggcagca aattgtccac attctttgag
     4921 agctgcggtg ttgcggatct catcacgaca tgttacggtg aggccgcgcg cgaatacagt
     4981 tgatatgaat gcacatcttt aattcatgtg caaacatttt ccaggtggtc gcaatcgtcg
     5041 tgtctcagag gcttttgtca catcgggcaa gacaatcgaa gatctggaga aagagatgct
     5101 caatggccaa aaattgcagg gcccgcccac tgccgaggag gttaactata tgttgaaaaa
     5161 caaaggcctg gaggataagt gcgtccactt tgtatatcaa actaaaatcg tatttaacaa
     5221 attgtttata tttatatact tgcagattcc cattgttcac cgccatacac aaaatttgca
     5281 caaatcagct taagcctaaa gatttaatcg attgcatacg caatcatccc gagcatatgt
     5341 aagtgttgag tttttgtttc ttgttttgct ttttgcacag ctgcacatga tatctccaag
     5401 agttccgtat tactttcccg taaatccctt cctgtctatg cctacgtccc acacacacac
     5461 gaacccacaa ccttttgagt ttttccgtat tttgtgttta atttgcaaat taatttcctt
     5521 tcttgtacca accaatttat gttaattaat tgtttttagt tatgcctatt taaatttagt
     5581 tttgtgcact cctcaataac ttttagcatg ttttgttgat ttaatatatc ttctgattaa
     5641 gttaacaatt taatataaca aaatatgaac aataaaactc gaaaacaaaa ttctaaatta
     5701 tcaaattgta aaatactttc aaaaaaaaag cttttaaata atatatatat ttttctttta
     5761 gaaattatcg ctcattcaca attccccttt cataatagct catacatatt aaaccctaca
     5821 tctaatccca atccaactaa tagatcccgc atatcatgta catttctaaa aacaagcaaa
     5881 aacaatcaac gacttttatg tttcatcaac aaaacaattt gttttcttct cttctttttt
     5941 atatgatttc tcatagggat acgttcatca tgccgtcgcc aaaactctaa actgtgttaa
     6001 accaaaacaa aaaaatgttc taaaagctta attcgattgt aaatactatt tgtttatata
     6061 tcaagtcaag tcaagtcaag tccagtcata cacacacaaa atattatcat gcacatccac
     6121 acacgcattt gcaaccgata cgtattcgaa aatcgtttcc ttcgccagct caacacgcat
     6181 tttataatac aactcgattc tatttaaaat ggttttcaaa aaccaatgta tgtgtcatag
     6241 gtgaaaatgt cagtgtcata tttgttaaac caaaatcagt tttgttaaac acgaataaag
     6301 tatttaaata ttgtttttgt tctgtacata tcaaaagaaa aacgcataat tagcattaaa
     6361 aaaaacatac ttatttttga aatgaaacat attttttgaa gaaattggca gctatacaaa
     6421 tcggcagcga gcagtacgta tcaaaatttg atatacttat tattaattga attttacgtt
     6481 atttgtttaa ctcacgtttg tatttgaatg caatttgaaa tgttacgctt attaaagcca
     6541 agcactttta taaataatga gttttgtttt ctattccgca ggcaaacttt gtaaaatata
     6601 tattaaaaca caaatttcta aacgttttta tgcaccaaaa acaaaaaaca aaaaaaaaac
     6661 caaacacaag caaatagtca ttttttttag cacacatttt aaaatcctca atacgccagg
     6721 caacgacaag aatggcatat acataggttg ttaatttatt gttaaaatcc aacgcatatc
     6781 cagcgaacca cagaaacgaa caaaaaccaa tcttgaagtc tgctttttgt taacttagtt
     6841 tataaacgat aaattagcaa atgaaattgt gcgcatgtac ctaaagacaa ttgtaaatca
     6901 attttacaat tacaattaaa aacaacagaa gaggcgtaat aaacggaaca acaatggttt
     6961 aatttaaaaa ttaaaaactt agacatacgg aattttattt gcattagtta attctactgc
     7021 aatctaaagt cataaataaa atgtaaaata aaaatgtgtt gaataaataa aagacaaaac
     7081 aagtactcag catttatttt ggaatagatt ttataaaggc tgcagggagt ttttgaaccg
     7141 aaccgacttg atcttaaaag tttgtcaaaa atctaattat acaaaataaa ttatttaaaa
     7201 aaatgcttac aaatatgcat atcaagtttt gaatcaagct gggcttggag ttgtggaaat
     7261 cgggttctgt aacccaagac gagaaaatgc ctcgcaccgt gaaccgaact acaaaaaagg
     7321 tttattaggt ttgcaaccta agcatattct ttacttggcg ccttagttca aatatacgtg
     7381 agaaactatc gat
//