From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: E.coli gcpE gene
Date: 28 Dec 92 21:28:15 GMT
Distribution: bionet
Checksum: 49776 4

LOCUS       ECOGCPE      1697 bp ds-DNA             BCT       01-DEC-1992
DEFINITION  E.coli gcpE gene
ACCESSION   X64451
KEYWORDS    gcpE gene.
SOURCE      Escherichia coli DNA.
  ORGANISM  Escherichia coli
            Prokaryotae; Gram-negative facultatively anaerobic rods;
            Enterobacteriaceae.
REFERENCE   1  (bases 1 to 1697)
  AUTHORS   Baker,J., Franklin,D.B. and Parker,J.
  TITLE     Sequence and characterization of the gcpE gene of Escherichia coli
  JOURNAL   FEMS Microbiol. Lett. 94, 175-180 (1992)
  STANDARD  full automatic
COMMENT     *source: strain=K12; *source: cell_line=JF298; *source:
            clone_library=lambda transducing phage dguaB-2; *source: sub_clone=
            pSE411;
               See also J01629 & M11843
               From EMBL    entry ECGCPE;  dated 28-JUL-1992.
FEATURES             Location/Qualifiers
     CDS             <1..508
                     /note="ORF"
                     /codon_start=2
                     /translation="TTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSP
                     SQANVDTAATPAPTAATTPDGAAPLPTDQAGVTTPVADPNALVMNFTADCWLEVTDAT
                     GKKLFSGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTL
                     NAEQSPAQ"
     CDS             535..1653
                     /gene="gcpE"
                     /codon_start=1
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQQVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
BASE COUNT      417 a    446 c    469 g    365 t
ORIGIN      
        1 gacaactaca gacccggcta caacgtccac gccgccagcg tctgtggata ctaccgcaac
       61 caacacgcaa acacctgccg taactgcgcc agcaccagct gttgatccgc aacagaatgc
      121 ggttgtttcg ccttcgcagg caaatgttga taccgccgcg accccggcac caacggcagc
      181 aacaacgcca gatggtgctg cgccgttgcc aaccgatcag gctggcgtga ccacgccggt
      241 ggctgatccg aatgcgctgg tgatgaactt tactgccgat tgctggctgg aggtcactga
      301 tgctaccggt aaaaaattgt ttagcggtat gcagcgtaaa gacggtaatt tgaacttaac
      361 cggccaggca ccgtacaaac tgaaaattgg tgcgccagcc gcagtacaga tccagtatca
      421 agggaaacct gtcgatctga gtcgttttat cagaactaac caggttgcgc gtctgaccct
      481 caatgccgaa caatcaccgg cgcagtaaca gacgggtaac gcgggagatt tttcatgcat
      541 aaccaggctc caattcaacg tagaaaatca acacgtattt acgttgggaa tgtgccgatt
      601 ggcgatggtg ctcccatcgc cgtacagtcc atgaccaata cgcgtacgac agacgtcgaa
      661 gcaacggtca atcaaatcaa ggcgctggaa cgcgttggcg ctgatatcgt ccgtgtatcc
      721 gtaccgacga tggacgcggc agaagcgttc aaactcatca aacagcaggt taacgtgccg
      781 ctggtggctg acatccactt cgactatcgc attgcgctga aagtagcgga atacggcgtc
      841 gattgtctgc gtattaaccc tggcaatatc ggtaatgaag agcgtattcg catggtggtt
      901 gactgtgcgc gcgataaaaa cattccgatc cgtattggcg ttaacgccgg atcgctggaa
      961 aaagatctgc aagaaaagta tggcgaaccg acgccgcagg cgttgctgga atctgccatg
     1021 cgtcatgttg atcatctcga tcgcctgaac ttcgatcagt tcaaagtcag cgtgaaagcg
     1081 tctgacgtct tcctcgctgt tgagtcttat cgtttgctgg caaaacagat cgatcagccg
     1141 ttgcatctgg ggatcaccga agccggtggt gcgcgcagcg gggcagtaaa atccgccatt
     1201 ggtttaggtc tgctgctgtc tgaaggcatc ggcgacacgc tgcgcgtatc gctggcggcc
     1261 gatccggtcg aagagatcaa agtcggtttc gatattttga aatcgctgcg tatccgttcg
     1321 cgagggatca acttcatcgc ctgcccgacc tgttcgcgtc aggaatttga tgttatcggt
     1381 acggttaacg cgctggagca acgcctggaa gatatcatca ctccgatgga cgtttcgatt
     1441 atcggctgcg tggtgaatgg cccaggtgag gcgctggttt ctacactcgg cgtcaccggc
     1501 ggcaacaaga aaagcggcct ctatgaagat ggcgtgcgca aagaccgtct ggacaacaac
     1561 gatatgatcg accagctgga agcacgcatt cgtgcgaaag ccagtcagct ggacgaagcg
     1621 cgtcgaattg acgttcagca ggttgaaaaa taataacgtg atgggaagcg cctcgcttcc
     1681 cgtgtatgat tgaaccc
//