From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Bovine respiratory syncytial virus fusion protein (F), and M2
Date: 29 Jul 92 10:01:20 GMT
Distribution: bionet
Checksum: 13040     9

LOCUS       BRSFPR       2907 bp ss-mRNA            VRL       29-JUL-1992
DEFINITION  Bovine respiratory syncytial virus fusion protein (F), and M2
            protein, complete cds, and RNA-dependent RNA polymerase mRNA, 5'
            end.
ACCESSION   M82816
KEYWORDS    L protein; M2 protein; RNA dependent RNA polymerase;
            fusion protein F.
SOURCE      Bovine respiratory syncytial virus cDNA to mRNA.
  ORGANISM  Bovine respiratory syncytial virus
            Viridae; ss-RNA enveloped viruses; Negative strand RNA viruses;
            Paramyxoviridae; Pneumovirus.
REFERENCE   1  (bases 1 to 2907)
  AUTHORS   Zamora,M. and Samal,S.K.
  TITLE     Sequence analysis of M2 mRNA of Bovine Respiratory Syncytial Virus
            obtained from an F-M2 dicistronic mRNA suggests structural homology
            with that of Human Respiratory Syncytial Virus
  JOURNAL   Unpublished (1991)
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     mRNA            1..1894
                     /gene="fusion protein F"
                     /note="putative"
                     /citation=[1]
     misc_signal     1..9
                     /note="transcription initiation signal of F protein"
                     /gene="fusion protein F"
                     /note="putative"
                     /citation=[1]
     CDS             14..1732
                     /product="fusion protein F"
                     /gene="fusion protein F"
                     /note="putative"
                     /codon_start=1
                     /translation="MATTTMRMIISIILISTYVPHITLCQNITEEFYQSTCSAVSRGY
                     LSALRTGWYTSVVTIELSKIQKNVCNGTDSKVKLIKQELERYNNAVAELQSLMQNEPT
                     SSSRAKRGIPESIHYTRNSTKKFYGLMGKKRKRRFLGFLLGIGSAIASGVAVSKVLHL
                     EGEVNKIKNALLSTNKAVVSLSNGVSVLTSKVLDLKNYIDKELLPKVNNHDCRISNIA
                     TVIEFQQKNNRLLEIAREFSVNAGITTPLSTYMLTNSELLSIINDMPITNDQKKLMSV
                     CQIVRQQSYSIMSVLREVIAYVVQLPLYGVIDTPCWKLHTSPLCTTDNKEGSNICLTR
                     TDRGWYCDNAGSVSFFPQAETCKVQSNRVFCDTMNSLTLPTDVNLCNTDIFNSKYDCK
                     IMTSKTDISSSVITSIGAIVSCYGKTKCTASNKNRGIIKTFSNGCDYVSNKGVDTVSV
                     GNTLYYVNKLEGKALYIKGEPIINYYNPLVFPSDEFDASIAQVNAKINQSLAFIRRSD
                     ELLHSVDVGKSTTNVVITTIIIVIVVVILMLITVGLLFYCKTRSTPIMLGKDQLSSIN
                     NLSFSK"
                     /citation=[1]
     misc_RNA        1882..1894
                     /note="transcription termination signal of F protein"
                     /gene="fusion protein F"
                     /note="putative"
                     /citation=[1]
     misc_feature    1895..1955
                     /note="F-M2 intergenic region"
                     /evidence=EXPERIMENTAL
                     /citation=[1]
     misc_RNA        1948..1956
                     /note="transcription initiation signal of M2 protein"
                     /gene="M2 (22K) protein"
                     /note="putative"
                     /citation=[1]
     mRNA            1955..2907
                     /gene="M2 (22K) protein"
                     /note="putative"
                     /citation=[1]
     CDS             1957..2517
                     /product="M2 (22K) protein"
                     /gene="M2 (22K) protein"
                     /note="putative"
                     /codon_start=1
                     /translation="MSRRNPCKYEIRGHCLNGKKCHFSHNYFEWPPHALLVRQNFMLN
                     KILKSMDRNNDTLSEISGAAELDRTEEYALGVIGVLESYLSSINNITKQSACVAMSKL
                     LAEINNDDIKRLRNKEVPTSPKIRIYNTVISYIDSNKRNTKQTIHLLKRLPADVLKKT
                     IKNTIDIHNEINGNNQGDINVDEQNE"
                     /citation=[1]
     CDS             2495..2782
                     /note="ORF2 of M2 protein"
                     /product="M2 (22K) protein"
                     /gene="M2 (22K) protein"
                     /note="putative"
                     /codon_start=1
                     /translation="MLMNKMNNSNIIIFPEKYPCSISSLLIKDENDVFVLSHQNVLDC
                     LQFQYPYNMYSQNHMLDDIYWTSQELIEDVLKILHLSGISINKYVIYVLVL"
                     /citation=[1]
     misc_RNA        2841..2849
                     /function="Transcription initiation signal of L protein
                     mRNA"
                     /standard_name="Gene-start sequence"
                     /gene="L protein"
                     /note="putative"
                     /citation=[1]
     CDS             2848..>2907
                     /standard_name="L protein"
                     /product="RNA-dependent RNA polymerase"
                     /gene="L"
                     /note="putative"
                     /codon_start=1
                     /translation="MDTLIHENSTNVYLTDSYLK"
                     /citation=[1]
     misc_RNA        2896..2907
                     /function="Transcription termination signal of M2 protein
                     mRNA"
                     /standard_name="Gene-end sequence"
                     /gene="M2 (22K) protein"
                     /note="putative"
                     /citation=[1]
BASE COUNT     1074 a    482 c    516 g    835 t
ORIGIN
        1 ggggcaaata aggatggcga caacaaccat gaggatgatc atcagcatta tcctcatctc
       61 tacctatgtg ccacatatca ctttatgcca gaacataaca gaagaatttt atcaatcgac
      121 atgcagtgca gttagtagag gttaccttag tgcattaaga actggatggt atacaagtgt
      181 ggtaacaata gagttgagca aaatacaaaa aaatgtatgt aacggtactg attcaaaagt
      241 gaaattaata aagcaagaac tagaaagata caacaatgca gtagcggaat tgcaatcact
      301 tatgcaaaat gaaccgacct cctctagtag agcaaaaaga gggataccag agtcgataca
      361 ttatacaaga aactctacaa aaaagtttta tggactaatg ggcaaaaaga gaaaaaggag
      421 atttttagga ttcttgctag gtattggatc tgctattgca agtggtgtag cagtgtccaa
      481 agtactacac ttggagggag aggtgaacaa aattaaaaat gcactgctat ccacaaataa
      541 agcagtggtt agtctgtcca atggagttag tgtccttact agcaaagtac ttgatctaaa
      601 gaactatata gacaaagagc ttctacctaa agttaacaat catgattgta ggatatccaa
      661 catagcaact gtgatagaat tccaacaaaa aaacaataga ttgttggaaa ttgctaggga
      721 atttagtgta aatgctggta ttaccacacc cctcagtaca tacatgttaa ccaatagtga
      781 gttactatca ataattaatg atatgcctat aacgaatgac caaaaaaagc taatgtcagt
      841 atgtcaaata gtcaggcaac agagttattc catcatgtca gtgttaagag aggtcatagc
      901 ttatgttgta caattgcctc tttatggagt tatagacacc ccctgttgga aactacacac
      961 ctctccatta tgcaccactg ataataaaga agggtcaaac atctgcttaa ctaggacaga
     1021 tcgtgggtgg tattgtgata atgcaggctc tgtgtctttt ttcccacaag cagagacgtg
     1081 taaggtacaa tcaaacagag tgttctgtga cacaatgaac agtttaactt tgcctactga
     1141 tgttaactta tgcaacactg acatattcaa ttcaaagtat gattgtaaaa taatgacatc
     1201 taaaactgac ataagtagct ctgtgataac ttcaatagga gctattgtat catgctatgg
     1261 gaagacaaaa tgtacagcct ctaataaaaa tcgtggaatc ataaagactt tttccaatgg
     1321 gtgtgattat gtatcaaaca aaggagttga tactgtatca gttggtaaca cactatatta
     1381 tgtaaataaa ctagagggga aagcactcta tataaagggt gaaccaatta ttaattacta
     1441 taatccacta gtatttcctt ctgatgagtt tgatgcatca attgctcaag taaacgcaaa
     1501 aataaaccaa agcctggctt tcatacgtcg atctgatgag ttacttcaca gtgtagatgt
     1561 aggaaaatcc accacaaatg tagtaattac tactattatc atagtgatag ttgtagtgat
     1621 attaatgtta ataactgtgg gattactgtt ttactgtaag accaggagta cacctatcat
     1681 gttagggaag gatcagctta gtagtatcaa caatctttcc tttagtaaat gaaatgcata
     1741 atgtttacaa tcttaacctc agaatcataa atgtgatgag ccaaatttac tgatacattc
     1801 aaaagttcca tctgccaaga cctgcatctt tatcaggtct gcacaagcta accttacatt
     1861 ctatactcag ctctatgtta atagttatat aaaagtatta tattaatctc aagatcacct
     1921 atttaataac caatcattca aaaagatggg gcaaatatgt cacgaagaaa tccctgcaaa
     1981 tatgagatta ggggacattg cttaaatggt aaaaaatgcc attttagtca taattacttt
     2041 gaatggcctc cacatgcttt attagtgagg caaaatttta tgctaaataa gatattaaaa
     2101 tctatggaca ggaacaacga taccctgtca gaaataagtg gtgctgcaga attagataga
     2161 acagaagaat atgcattggg tgtgatagga gttttggaaa gttacttaag ctctatcaat
     2221 aatataacaa aacaatcagc ctgtgttgct atgagtaaac tattagccga gattaacaat
     2281 gatgacatca agagattaag gaacaaggaa gtgccaacat cacccaagat aagaatatat
     2341 aacacagtta tatcatatat tgatagcaac aagagaaaca caaaacaaac tatacatttg
     2401 cttaagagat tgcctgcaga cgtgcttaaa aagaccatca agaacacaat agatattcac
     2461 aacgaaataa atggtaataa ccaaggtgac ataaatgttg atgaacaaaa tgaataactc
     2521 caacattatt attttcccag aaaaataccc ctgtagcata tcctctttgc taattaagga
     2581 tgaaaatgat gtttttgtac taagtcatca gaatgttctt gactgcttac agtttcaata
     2641 tccatataat atgtattctc aaaatcatat gcttgatgat atctattgga catcacagga
     2701 gctgattgag gatgtactta agattcttca tctttctggc atatccataa ataagtatgt
     2761 gatatatgtt ttagtgctat agtatataag tcactcaact attgatcaac agccacttct
     2821 tcatagctag caatatataa ggacaaaatg gatacactca ttcatgagaa ctcaactaat
     2881 gtttacttaa cagatagtta tttaaaa
//