From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: L.esculentum gene for polyphenol oxidase.
Date: 25 Jul 92 10:07:04 GMT
Distribution: bionet
Checksum: 61733     5

LOCUS       TOMPOOXD     1982 bp ds-DNA             PLN       25-JUL-1992
DEFINITION  L.esculentum gene for polyphenol oxidase.
ACCESSION   Z12836
KEYWORDS    polyphenol oxidase.
SOURCE      Lycopersicon esculentum DNA.
  ORGANISM  Lycopersicon esculentum
            Eukaryota; Plantae; Embryobionta; Magnoliophyta; Magnoliopsida;
            Asteridae; Solanales; Solanaceae.
REFERENCE   1  (bases 1 to 1982)
  AUTHORS   Newman,S.M.
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
REFERENCE   2  (bases 1 to 1982)
  AUTHORS   Newman,S.M., Eannetta,N.T., Yu,H., Prince,J.P., de Vicente,C.,
            Tanksley,S.D. and Steffens,J.C.
  TITLE     Organization of the tomato polyphenol oxidase gene family
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
COMMENT     *source: strain=cv. VFNT cherry; *source: clone_library=Charon 35
            library of R. Fischer; *source: chromosome=8; *source:
            is_macronuclear=N; *source: is_proviral=N; *source: is_germline=N;
            From EMBL    entry LEPOOXD;  dated 26-JUN-1992.
FEATURES             Location/Qualifiers
     CAAT_signal     4..9
     TATA_signal     102..106
     CDS             151..1926
                     /product="polyphenol oxidase precursor"
                     /codon_start=1
                     /translation="MASLCSNSSTTSLKTPFTSLGSTPKPCQLFLHGKRNKAFKVSCK
                     VTNTNGNQDETNSVDRRNVLLGLGGLYGVANAIPLAASAAPTPPPDLSSCSIARIDEN
                     QVVSYSCCAPKPDDMEKVPYYKFPSMTKLRVRQPAHEADEEYIAKYNVSVTKMRDLDK
                     TQPLNPIGFKQQANIHCAYCNGAYRIGGKELQVHNSWLFFPFHRWYLYFYESNAGKLI
                     DDPTFALPYWNWDHPKGMRFPAMYDREGTFLFDETRDQSHRNGTVIDLGFFGNEVETT
                     QLQMMSNNLTLMYRQMVTNAPCPRMFFGDLMISGITLNSPGTIENIPHGPVHIWSGTV
                     RGSTLPNGAISKRGEYGHFYSAGLDPVFFCHHSNVDRMWSEWKATGGKRTDITHKDWL
                     NSEFFFYDENENPYRVKVRDCLDTKKMGYDYKPMRTPWRNFKPLTKASRGKVNTSSIP
                     PVSQAFPLAKLDKAVSFSINRPTSSRTPQEKNAQEEMLTFNSIRYDNRGYIRFDVFLN
                     VDNNVNANELDKAEFAGSYTSLPHVHRAGETNHIATVDFQLAITELLEDIGLEDEDTI
                     AVTLVPKRGGEGISIENATISLADC"
     transit_peptide 151..399
     mat_peptide     400..1923
                     /product="polyphenol oxidase"
     misc_binding    730..840
                     /bound_moiety="copper, tyrosinase-like CuA bindingsite"
     misc_binding    1120..1278
                     /bound_moiety="copper, tyrosinase-like CuB bindingsite"
BASE COUNT      580 a    402 c    438 g    562 t
ORIGIN
        1 attcaaaatt ccccacctaa cgcgtcctgg aataattgat atgacttgaa gccgcctcta
       61 aaattaaata ataattggtg cttataatgt tttacatatt atataaagca aggtatagcc
      121 caatgaattt tcattcaaaa gctagcaaca atggcaagtt tgtgtagtaa tagtagtact
      181 acttctctca aaactccttt cacttcttta ggttccactc caaagccttg tcaacttttc
      241 ctacatggaa aacgtaacaa agcattcaaa gtttcatgca aggttaccaa tactaacggt
      301 aaccaagatg aaacgaattc tgtagatcga aggaatgttc ttcttggctt aggaggtctt
      361 tatggtgttg ctaatgctat accattagct gcatcggctg ctcctacacc acctcctgat
      421 ctctcgtctt gtagtatagc aaggattgac gagaatcagg tggtgtcata cagttgttgc
      481 gcgcctaagc ctgatgatat ggagaaagtt ccgtattaca agttcccttc tatgactaag
      541 ctccgtgttc gtcagcctgc tcatgaggct gatgaggagt acatcgccaa gtacaacgtg
      601 tcggttacga agatgagaga tcttgataag acacaacctt tgaaccctat tggttttaag
      661 caacaagcta atatacattg tgcttattgt aacggtgctt atagaattgg tggcaaagag
      721 ttacaagttc ataactcgtg gcttttcttc ccgttccata gatggtactt gtacttctac
      781 gagagtaatg cgggaaaact gattgatgat ccaactttcg ctttgcccta ttggaattgg
      841 gatcatccaa agggtatgcg ttttcctgcc atgtatgatc gtgaagggac ttttcttttc
      901 gatgaaacac gtgaccaaag tcaccgtaat ggaaccgtca ttgatcttgg attttttggc
      961 aatgaagtcg aaacaactca gctacagatg atgagtaata acttaactct aatgtaccgt
     1021 caaatggtga ctaatgctcc atgtcctcgg atgttctttg gcgaccttat gatctcgggc
     1081 ataacgttga actccccggg aactatagag aacatccctc acggtcctgt gcacatttgg
     1141 tctggtacag tgagaggttc aactttgccc aatggagcca tatcaaaacg gggagaatat
     1201 ggacatttct attccgctgg tttagacccg gttttctttt gccatcacag caatgtggat
     1261 cgaatgtgga gcgaatggaa agcaacagga gggaaaagaa ccgatatcac acataaagat
     1321 tggttgaact ccgaattctt cttctacgat gaaaatgaaa atccttaccg tgtgaaagtc
     1381 agggactgct tggacacgaa gaagatgggg tacgattaca agccaatgcg aaccccatgg
     1441 cgtaacttca agcctttaac aaaggcttca cgtgggaaag taaatacaag ttcaattcct
     1501 ccagtaagcc aggcattccc attggcgaaa ctcgacaaag cagtttcgtt ttccatcaac
     1561 aggccgactt catcgcggac tccacaagag aaaaatgcac aagaggagat gctaacattc
     1621 aacagcataa gatatgataa cagagggtac ataaggttcg atgtgttcct gaacgtggac
     1681 aacaatgtga atgcgaatga gcttgataag gcagagttcg cggggagtta tactagtttg
     1741 ccacatgttc atagagctgg tgagactaat cacatcgcca cggttgattt ccagctggcc
     1801 ataacagaac tgttggagga tattggtttg gaagatgaag acacaattgc ggtgactctg
     1861 gtgccaaaga gaggtggaga aggtatctcc attgaaaatg cgaccatcag tcttgctgat
     1921 tgttaattag ttactatcga atttgaatct gctgatatta ctttgatgct ctgtttctgt
     1981 tt
//