From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: D.pseudoobscura Adh gene for alcohol dehydrogenase
Date: 28 Dec 92 21:26:18 GMT
Distribution: bionet
Checksum: 38665 8

LOCUS       DROADHGN05   3498 bp ds-DNA             INV       01-DEC-1992
DEFINITION  D.pseudoobscura Adh gene for alcohol dehydrogenase
ACCESSION   X62185
KEYWORDS    ADH gene; alcohol dehydrogenase.
SOURCE      Drosophila pseudoobscura DNA.
  ORGANISM  Drosophila pseudoobscura
            Eukaryota; Animalia; Metazoa; Arthropoda; Uniramia; Insecta;
            Pterygota; Neoptera; Holometabola; Diptera; Brachycera;
            Cyclorrhapha; Schizophora; Drosophiloidea; Drosophilidae.
REFERENCE   1  (bases 1 to 3498)
  AUTHORS   Schaeffer,S.W. and Miller,E.L.
  TITLE     Estimates of gene flow in Drosophila pseudoobscura determined from
            nucleotide sequence analysis of the alcohol dehydrogenase region
  JOURNAL   Genetics 132, 471-480 (1992)
  STANDARD  full automatic
COMMENT     *source: strain=BN16; *source: isolate=British Columbia, Canada;
            *source: chromosome=IV section 88;
               From EMBL    entry DPADHGN05;  dated 16-OCT-1992.
FEATURES             Location/Qualifiers
     mRNA            join(40..119,923..1028,1092..1496,1557..1990)
                     /note="adult mRNA (Adh)"
     mRNA            join(890..1028,1092..1496,1557..1990)
                     /note="larval mRNA (Adh)"
     promoter        8..14
                     /note="adult promoter (Adh)"
     prim_transcript 40..1990
                     /note="adult primary transcript (Adh)"
     exon            40..119
                     /note="adult exon 1a"
     intron          120..922
                     /note="adult intron"
     promoter        854..861
                     /note="larval promoter (Adh)"
     prim_transcript 890..1990
                     /note="larval primary transcript (Adh)"
     exon            890..1028
                     /note="larval exon 1"
     exon            923..1028
                     /note="adult exon 1b"
     intron          1029..1091
                     /number=1
     exon            1092..1496
                     /number=2
     intron          1497..1556
                     /number=2
     exon            1557..1990
                     /number=3
     polyA_signal    1967..1972
     polyA_site      1990
     CDS             join(936..1028,1092..1496,1557..1823)
                     /EC_number="1.1.1.1"
                     /gene="Adh"
                     /product="alcoholdehydrogenase"
                     /codon_start=1
                     /translation="MSLTNKNVVFVAGLGGIGLDTSRELVKRNLKNLVILDRIDNPAA
                     IAELKAINPKVTITFYPYDVTVPVAETTKLLKTIFAQVKTIDVLINGAGILDDHQIER
                     TIAVNYTGLVNTTTAILDFWDKRKGGPGGIICNIGSVTGFNAIYQVPVYSGSKAAVVN
                     FTSSLAKLAPITGVTAYTVNPGITKTTLVHKFNSWLDVEPRVAEKLLEHPTQTSQQCA
                     ENFVKAIELNKNGAIWKLDLGTLEPITWTQHWDSGI"
BASE COUNT      978 a    797 c    801 g    922 t
ORIGIN      
        1 cgtagtgtat ttaaggcact gcacatgtcg aggaccacga ttattgtcta cagtgcagtc
       61 agtagttgtc aggtgcagca ggcggaccag catttcttta aatctactca atcgatcaag
      121 taagtaactg taatccaagt acagtcggcc catcccactc ggtcactcgg ttctgccgta
      181 actcaagaaa tttcttgata gcacagttcc agttgtaaaa cgtcgtctaa aaaaacatct
      241 cgttggagct tgggttgaaa aacgttcgag ttgggcgtag aaaagttcaa atataaaata
      301 tcaaaaaaga gaaaatatct tcccaaagca gctgtccatt ttttgttaaa gtgatagatc
      361 aaaaattcgt atgacgtatt tcccaagtgc tctttatgca aacaatttcc tcaattctaa
      421 ttctacaaga agcacttttg gctcttgtcc agaaaaaaca atttgtttac acttttcggt
      481 tgataacaga ggcggaccga ccgctaagta acataacagt cgcctgttaa gtaatgccct
      541 cactctctgt tagatggccg cgcgtgtcga ctccgataga ccagcagaca cgcagtagtt
      601 ttcctcaaga acctgaactc taaacataga cataatttac gataccttga gactcaaata
      661 aaaatacaag tatgagtggt attctattcc tgattagact cgcacacaca cacgtacgag
      721 tattaacata aatttgccgg caaataacca aaacaaaacg aacgcaaacg atccgaacac
      781 gtaatgcgag agataagaaa cagaaaagct cgacgtgaaa gaagcttctg gcggaagctc
      841 tggccgaact ccctataaat acgagacaga aaaccagaat aaatataata acaaactgtt
      901 gaattgtccc atcgattccc agatcaaaag acagaatgtc tctcaccaac aagaacgtcg
      961 ttttcgtggc cggtctgggc ggcattggcc tggacaccag ccgggagttg gtcaagcgta
     1021 atctgaaggt aagagagaac gaattccatg gaatctatgg aatcctaaat ttaaaaattc
     1081 atttatttta gaacctggtc atcctggatc gcattgacaa tccggctgcc attgccgaac
     1141 tgaaggcaat caatcccaag gtgaccatca ccttctatcc ctacgatgtg actgtgcccg
     1201 tcgctgagac caccaagctc ctgaagacca tctttgccca ggtgaagaca atcgatgtcc
     1261 tgatcaacgg tgctggcatc ctggacgatc atcagatcga gcgcaccatt gccgttaact
     1321 acacgggcct ggtcaacacc accacagcca ttctggactt ctgggacaag cgcaagggcg
     1381 gcccaggcgg catcatttgc aacattggct ccgtcaccgg tttcaatgcc atctaccagg
     1441 tgcccgttta ctccggctcc aaggcggcgg tggttaactt tacctcctcc ctggcggtaa
     1501 gtaatccaac gtttttccta ctccccaaaa ctaaatattt cttcgaaatc ccatagaaac
     1561 tggctcccat tactggtgtc actgcttaca ctgtcaatcc tggcatcact aagaccactc
     1621 tggtccacaa attcaactcg tggctggatg tggagccccg tgtggcggag aagctgctcg
     1681 agcatcccac ccagacctct cagcagtgcg ccgagaactt tgtgaaggcc attgagctga
     1741 acaagaacgg tgccatctgg aagttggatt tgggcacctt ggagcccatc acatggaccc
     1801 agcactggga ctcgggcatc taaaccatcc cagagactct atgggacatg gcgttttagc
     1861 ttttagtttc tttttttcca ctcaattgtt acgtatatac atacacatat ggcaataagg
     1921 ctgatttgat tctcttttat ggaaggaacc ccgttttgaa tatgataata caaaattata
     1981 tttgagaaat ttcaacaaaa tcgataagag ctatagctct cttttaatta aaaaaaacta
     2041 gataaaaaga gcaatggtca gtggtggtca gtggtcgttg tggcctctat cgatttccac
     2101 acaaaaactt tactgtttag taatagaaaa gaacttgaga aaggcagcca aaagaatgta
     2161 cgatctgacg ggtaagcatg tctgctatgt ggctgactgc ggtggcattg cactggagac
     2221 cagcaaggtt ctcatgacca agaatatagc ggtgagtggt agagaggtgg tgtagagagt
     2281 ggagagtgtt ccgaagtggt tctggttctt aaacaagtct ttagtctctt ggctgctgct
     2341 ggtagctgtc gaaactaatt aagttaatga ctttttcatg ttttgttttt gtccgctgac
     2401 ttttgctgtt caaagctaca attaagttta tgttttgagt ttgatctact tttgcttaag
     2461 ggaagtactt tcgattacgg atcgaattgc tggcatataa aaatagatac caaggaatgt
     2521 gcatcatatt gattctatct tgattctttg cctagaaact ggccatcctt cagagcgtgg
     2581 aaaacccccc ggccatcgct cagttacaat ctattaagca cagcacacag atcttcttct
     2641 ggaccttcga tgtgaccatg gcccgcgagg agatgaagaa gtacttcgat gaagtcatgg
     2701 tccagatgga ctacatcgat gtgctgatca atggggccac cctgtgcgat gaacggaaca
     2761 ttgatgccac catcaatacg aatctgaccg gcatgatgaa caccgtggcc actgtgctgc
     2821 cctatatgga ccgtaagatg ggcggttcgg gtggactgat tgtgaatgtc acctccgtca
     2881 taggactgga tccatcgcca gtcttttgtg cgtacagtgc ctcaaagttt ggtgtgattg
     2941 ggttcacacg aagtctagcg gtgagtgggc gagatcttaa cttgaaattg ctctctaatg
     3001 tcttccattc tttcgcagga tcccctgtat tacacccaaa atggtgtggc tgtcatggcc
     3061 gtctgctgtg gtcccaccaa agtgtttgtc gatcgggaac tgactgcctt cctggaatac
     3121 ggccagtcct ttgccgatcg cctgcgtcgt gccccctgcc aatcgacggc cgtctgcggc
     3181 cagaatatag taaatgccat cgagagatcg gagaacggac agatctggat tgccgacaag
     3241 ggcggcctgg agtcggtggc cctccactgg tattggcata tggccgatca gtttgtgaac
     3301 tatatgcaga gcactgacga tgaggatcag gagttttttt tgggccagcg atgaggcatg
     3361 ccttgatcca cggaataaaa catcaaatgt gccacaaaaa gtaggcaact tgtttgtggg
     3421 tctggaaata gatttgaaat attttgaggt catatgaata atgacataaa tgagggtgcc
     3481 tgaaagtatg ccaagttt
//