From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Serratia marcescens aminoglycoside acetyltransferase (aac(6')-Ic)
Date: 6 Nov 92 18:35:38 GMT
Distribution: bionet
Checksum: 62125 5

LOCUS       SMAAACGIC    2057 bp ds-DNA             BCT       02-NOV-1992
DEFINITION  Serratia marcescens aminoglycoside acetyltransferase (aac(6')-Ic)
            gene, complete cds.
ACCESSION   M94066
KEYWORDS    6'-N-acetyltransferase; amikacin resistance;
            aminoglycoside 6'-N-acetyltransferase;
            aminoglycoside acetyltransferase; aminoglycoside resistance.
SOURCE      Serratia marcescens DNA.
  ORGANISM  Serratia marcescens
            Prokaryotae; Gram-negative facultatively anaerobic rods;
            Enterobacteriaceae.
REFERENCE   1  (bases 1 to 2057)
  AUTHORS   Shaw,K.J., Rather,P.N., Sabatelli,F.J., Mann,P., Munayyer,H.,
            Mierzwa,R., Petrikkos,G.L., Hare,R.S., Miller,G.H., Bennett,P.M.
            and Downey,P.
  TITLE     Characterization of the chromosomal aac(6')-Ic gene from Serratia
            marcescens
  JOURNAL   Antimicrob. Agents Chemother. 36, 1447-1455 (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     -35_signal      1481..1486
                     /gene="aac(6')-Ic"
     -10_signal      1504..1509
                     /gene="aac(6')-Ic"
     CDS             1554..1994
                     /gene="aac(6')-Ic"
                     /product="aminoglycoside acetyltransferase"
                     /codon_start=1
                     /translation="MIVICDHDNLDAWLALRTALWPSGSPEDHRAEMREILASPHHTA
                     FMARGLDGAFVAFAEVALRYDYVNGCESSPVAFLEGIYTAERARRQGWAARLIAQVQE
                     WAKQQGCSELASDTDIANLDSQRLHAALGFAETERVVFYRKTLG"
BASE COUNT      412 a    644 c    604 g    397 t
ORIGIN      
        1 ctgcagttcc tggatctgat tcaggaaggc aacatcggcc tgatgaaagc ggtagacaag
       61 ttcgaatacc gccgcggcta caagttctcg acgtacgcca cctggtggat ccgtcaggct
      121 atcacccgct ccatcgccga ccaggcgcgc accatccgta ttccggtgca tatgattgag
      181 accatcaaca agctcaaccg tatttcgcgc cagatgctgc aagagatggg ccgcgagccg
      241 acgccggaag agctggccga gcgcatgctg atgccggaag acaagatccg caaagtgctg
      301 aagatcgcca aagagccaat ctcgatggag acgccgatcg gcgacgacga agattcgcat
      361 ctgggcgact tcatcgagga taccaccctc gagctgccgc tggattccgc gacctcggaa
      421 agcctgcgtt ccgccaccca cgacgtgctg gccggcctga ccgcgcgcga acgaaagtgc
      481 tgcgcatgcg tttcggtatc gacatgaaca ccgaccacac gctggaagag gtaggcaagc
      541 agtttgacgt tacccgtgag cgtatccgtc agatcgaagc caaggcgctg cgcaagctgc
      601 gtcacccaag ccgctccgaa gtgctgcgca gcttcctgga cgactaagtc cggctgccgc
      661 agcaaaaaca aaaaccccgg cctgccgggg tttttttatg ccctgaatac gcctcgccgc
      721 atcattgcag cgccaggaac agctcgcgat agctggcggt aagctgctcg agggtggcgc
      781 ggttcaatcc gctggggttg ggcaataccc acacctcggt ttcccccaac gtcagcgtct
      841 gcttgcccca gggcgcattt ttcaccccga aggcggtggt gaacgcctgc ttgcccaaaa
      901 tcgccagcgc gcgcggctga tagcgcagga tcttctcttg cagcgcctca ccgccgctac
      961 gcagctcgtc gcgcgacagc tcgctggccg ccaccgtcgg gcgcgccacc agcgcggtaa
     1021 tgccgcagcc gttgtccttc aactgctgcc actgctccgg cgccagctgg ctctcggtaa
     1081 agccggcctg atggatcacc ttccagaagc gattgctgcc gttggcgaaa ggatagccct
     1141 gatgggcgga agaaagaccg gggttgatgc cgcaaaacac cacccgcaga ttgggcgcca
     1201 gaagttccat gagtgtcgtg agtccattgc agggatttac tgcggttatc gcaaaatccg
     1261 ccagcaatta caacaggcta gaactgtgca gaaaagcaac atacgggcga gtaacagtag
     1321 accgccgctt aaggattccc tataatctcg cggcttggcc ccttagctca gtggttagag
     1381 caggcgactc ataatcgctt ggtcgttggt tcaaacccaa caggggccac caaatttttg
     1441 ctgttttcac agccttacaa gccatcctgt ccggggtggc ttttttgttt cccgcctgcg
     1501 tgctacactt ctctctcggc attctcgttt attccgaccc ataaggatcc cgcatgatcg
     1561 tcatctgcga ccacgacaac ctcgacgcct ggctggcgct gcgcaccgcg ctgtggccct
     1621 ccggctcgcc tgaagatcac cgcgcggaaa tgcgcgagat attggcttcg ccgcaccaca
     1681 ccgcgtttat ggcgcggggg ctggacggcg ctttcgttgc ctttgccgag gtcgcgctgc
     1741 gctacgatta cgtcaacggc tgcgaatcgt cgccggtggc gtttttggaa ggaatttata
     1801 ccgccgaacg cgcccgccgc cagggctggg ccgcgcgcct gatcgcgcag gtgcaggagt
     1861 gggcgaagca acaggggtgc agcgagctgg cgtcggatac cgatatcgcc aatctggact
     1921 cccagcgcct gcatgcggcg ctgggctttg ccgaaacgga gcgagtagtg ttttaccgca
     1981 aaacgctggg ctgatcaggc ggtcttgctt tgccgctgat gcgccagctg ctcggcgcgc
     2041 agcaggatct cctgcag
//