From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: E. coli DNA for orf17, orf26, ruvC and orf23 genes.
Date: 21 Jul 92 10:04:21 GMT
Article-I.D.: genbank.Jul.21.03.04.20.1992.18040
Distribution: bionet
Checksum: 62197    10

LOCUS       ECORUVC      3255 bp ds-DNA             BCT       21-JUL-1992
DEFINITION  E. coli DNA for orf17, orf26, ruvC and orf23 genes.
ACCESSION   D10165 D90392
KEYWORDS    Holliday junction-specific endonuclease; Holliday structure; RuvC;
            endonuclease.
SOURCE      Escherichia coli (strain K12) DNA.
  ORGANISM  Escherichia coli
            Prokaryotae; Gram-negative facultatively anaerobic rods;
            Enterobacteriaceae.
REFERENCE   1  (sites)
  AUTHORS   Shinagawa,H., Makino,K., Amemura,M., Kimura,S., Iwasaki,H. and
            Nakata,A.
  TITLE     Structure and regulation of the Escherichia coli ruv operon
            involved in DNA repair and recombination
  JOURNAL   J. Bacteriol. 170, 4322-4329 (1988)
  STANDARD  full staff_review
REFERENCE   2  (sites)
  AUTHORS   Benson,F.E., Illing,G.T., Sharples,G.J. and Lloyd,R.G.
  TITLE     Nucleotide sequencing of the ruv region of Escherichia coli K-12
            reveals a LexA regulated operon encoding two genes
  JOURNAL   Nucleic Acids Res. 16, 1541-1549 (1988)
  STANDARD  full staff_review
REFERENCE   3  (bases 1 to 3255)
  AUTHORS   Takahagi,M., Iwasaki,H., Nakata,A. and Shinagawa,H.
  TITLE     Molecular analysis of the Escherichia coli ruvC gene, which encodes
            a Holliday junction-specific endonuclease
  JOURNAL   J. Bacteriol. 173, 5747-5753 (1991)
  STANDARD  full staff_review
COMMENT     Data kindly submitted in computer readable form by:
            Masahiko Takahagi
            Reseach Institute for Microbiol Diseases
            Osaka University
            3-1 Yamadaoka, Suita
            Osaka 565
            Japan
            Phone:  06-877-5121
            Fax:    06-876-2678
            [1]  sites.
            [2]  sites.
FEATURES             Location/Qualifiers
     -35_signal      440..445
     repeat_region   450..481
                     /rpt_type=inverted
                     /rpt_unit=450..463
                     /rpt_unit=468..481
     -10_signal      463..468
     RBS             503..506
     CDS             511..963
                     /product="Orf17"
                     /codon_start=1
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     RBS             513..517
     CDS             523..963
                     /product="Orf17"
                     /codon_start=1
                     /translation="MYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVEEGET
                     APQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESWFCLA
                     LPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     -35_signal      924..929
     -10_signal      945..950
     RBS             980..983
     CDS             992..1732
                     /product="Orf26"
                     /codon_start=1
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     -35_signal      1668..1673
     -10_signal      1691..1696
     repeat_region   1704..1737
                     /rpt_type=direct
                     /rpt_unit=1704..1715
                     /rpt_unit=1726..1737
     RBS             1756..1759
     CDS             1767..2288
                     /function="endonuclease"
                     /product="RuvC"
                     /gene="ruvC"
                     /codon_start=1
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     repeat_region   1820..1864
                     /rpt_type=inverted
                     /rpt_unit=1820..1834
                     /rpt_unit=1850..1864
     repeat_region   2288..2329
                     /rpt_type=inverted
                     /rpt_unit=2288..2305
                     /rpt_unit=2311..2329
     CDS             complement(2290..2892)
                     /product="Orf23"
                     /codon_start=1
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTERQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGELLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     RBS             complement(2901..2903)
     -10_signal      complement(3041..3046)
     -35_signal      complement(3065..3070)
     -35_signal      3084..3089
     -35_signal      3089..3094
     misc_signal     3100..3115
                     /note="SOS box (LexA protein-binding sequence)"
     -10_signal      3105..3110
     -10_signal      3113..3118
     RBS             3156..3160
     CDS             3167..>3255
                     /product="RuvA"
                     /gene="ruvA"
                     /codon_start=1
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVH"
BASE COUNT      859 a    752 c    857 g    787 t
ORIGIN      41 min on K12 map.
        1 gaactgaaag ctgcaccgga aaatgcggtg gcgaacgctt acgatatggt catcaatggt
       61 tacgaagtgg gcggtggttc agtacgtatc cataatggtg atatgcagca gacggtgttt
      121 ggtattctgg gtatcaacga agaggaacag cgcgagaaat tcggcttcct gctcgacgct
      181 ctgaaatacg gtactccgcc gcacgcaggt ctggcattcg gtcttgaccg tctgaccatg
      241 ctgctgaccg gcaccgacaa tatccgtgac gttatcgcct tcccgaaaac cacggcggca
      301 gcgtgtctga tgactgaagc accgagcttt gctaacccga ctgcactggc tgagctgagc
      361 attcaggttg tgaagaaggc tgagaataac tgatatgact caaatacacg aaatcattcg
      421 cgttgcatcg aggcggcaac tgagtgaact cccatgagca tagataacta tgtgaatggg
      481 atgagcgaag gcagtcaacg aagaggcagc gtgaaggata aagtgtataa gcgtcccgtt
      541 tcgatcttag tggtcatcta cgcacaagat acgaaacggg tgctgatgtt gcagcggcgt
      601 gacgatcccg atttctggca gtcggtaacc ggcagcgtgg aagagggtga aaccgcgccg
      661 caagctgcca tgcgcgaagt aaaggaagag gtcaccattg atgttgtcgc tgaacaactg
      721 accttaattg actgtcagcg cacggtagag tttgaaattt tttcacattt acgtcatcgc
      781 tatgcgccgg gcgtgacgcg taatacggaa tcatggttct gtcttgcgct tccgcacgag
      841 cggcagatcg ttttcactga acatctggct tacaagtggc ttgatgcgcc tgctgcggcg
      901 gcgctcacta agtcctggag caaccggcag gcgattgaac agtttgtaat taacgctgcc
      961 tgaacaggca gggctatctg gagatatttt tatggcaggt catagtaaat gggccaacac
     1021 cagacatcgt aaagctgcgc aggatgctaa gcgcggtaaa atcttcacta aaatcattcg
     1081 tgagctggta accgcggcta agctgggcgg tggcgatccg gacgctaacc cgcgtctgcg
     1141 tgcggcggtg gataaagcac tgtctaacaa catgacccgt gacacactga accgcgcaat
     1201 tgcacgtggt gtgggcggtg atgatgatgc aaacatggaa accatcatct acgaaggtta
     1261 cggtcctggc ggcacggcaa tcatgattga atgtctgtct gacaaccgca accgtaccgt
     1321 tgctgaagtg cgtcatgcat ttagcaaatg tggcggtaac ctcggtactg atggttccgt
     1381 tgcctatctg ttcagcaaaa aaggcgtgat ctccttcgag aaaggcgatg aagacaccat
     1441 catggaagca gcactggaag caggtgctga agacgttgtg acctatgatg acggcgcgat
     1501 tgatgtctac actgcatggg aagaaatggg taaagtgcgc gacgctctgg aagcggcagg
     1561 tctgaaagca gacagcgcgg aagtttccat gatcccgtct accaaagctg atatggatgc
     1621 agaaaccgca ccgaaactga tgcgtctgat cgatatgctg gaagattgcg acgacgtgca
     1681 ggaagtttac cataacggtg aaatctctga tgaggtcgca gcgactctct gatgaggcct
     1741 gctaaacagc aaaacggaga cgcgtgatgg ctattattct cggcattgat ccgggttcgc
     1801 gcgtgaccgg ctacggcgtc atccgccagg taggtaggca actgtcctac ctgggtagcg
     1861 gatgcatccg caccaaagtg gatgatttac cgtctcgtct gaaactcatc tatgcgggcg
     1921 tgacggaaat catcacccag ttccagcctg attatttcgc cattgaacaa gtctttatgg
     1981 caaagaacgc tgactcagcc ctgaaactgg gccaggcgcg cggcgtggcg attgtggcgg
     2041 cggtgaatca ggagttgcca gtatttgaat acgcggcacg tcaggtaaag caaacggtgg
     2101 taggtattgg cagtgccgaa aaaagccagg tgcagcatat ggtccgcacc ttgctgaaac
     2161 tgcccgctaa tccacaggcg gatgccgccg atgcgctggc gattgctatc acccactgcc
     2221 acgttagtca gaatgcgatg cagatgagcg aatcgcggct gaacctggcg agagggcgac
     2281 tgcgttaagt tataccgcct cggtcagttc cggctgaggc gtttccactc cctccgcatg
     2341 aatcaacacc aaacccggat gatgccacaa actggctggc gtgacggttt tacgctccca
     2401 cggaatagaa cccaggaacc agaatttcca gaaagtgagt tttgcattcg gattgctatt
     2461 taacaattct ccaaacgtct ctatttcacc caccggaata catagcgctt ctttataaat
     2521 atcaaaaaca aattttgaac agaactggcg cgaagattcg tatttaaaac cggtgtggta
     2581 aagtttgcgt agccgggagg gaacctgttc aacaattcgt tgtttttgtc gttctgtcag
     2641 cccagcgtct aatcgcttta tagcatagcg ttgattagac gaacgtttaa taaaacgaga
     2701 tagcgtggtg atggttgaaa ggggaacccg gctttctgca accaggaagt cttcaccgtt
     2761 atgaccgata atgatcccga cgtgattact ccagcaattt gatgcagctg atatttgacc
     2821 aaataaggca gcacctatac atgtaaagac gatatctcca atttcatatt cagcagggta
     2881 attaatattc acgttgtcat tccattgaaa tagatacaaa tatacgtaat agcataaaaa
     2941 atttattttt tcaggaggcg caatgtatat ttttattacg catttcttca ctgaatatgt
     3001 aatattaaaa tatttgcttc caatataacc tgtagaataa attatactgt gccatttttc
     3061 agttcatcga gacacctcgc aagttttctt catccttcgc tggatatcta tccagcattt
     3121 ttttatcata cagcattatc tttgattcat tacgcaggag cgtcatgtga taggcagact
     3181 cagaggcatc atcattgaaa aacaaccccc gctggtgtta attgaagtgg gcggcgtagg
     3241 ctatgaagtg catat
//