From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Rice gene for Q-enzyme(starch branching enzyme), cds.
Date: 21 Jul 92 10:08:20 GMT
Distribution: bionet
Checksum: 20005     7

LOCUS       RICREQEI     2758 bp ss-mRNA            PLN       21-JUL-1992
DEFINITION  Rice gene for Q-enzyme(starch branching enzyme), cds.
ACCESSION   D10752
KEYWORDS    Q enzyme; a-1,4-glucan, a-1,4-glucan 6-glucosyltransferase;
            amylopectin biosynthesis; starch branching enzyme.
SOURCE      Oryza sativa (clone Bluescript, sub_species Fujihikari) (library:
            lamda gt11) milky stage endosperm cDNA to mRNA.
  ORGANISM  Oryza sativa
            Eukaryota; Plantae; Embryobionta; Magnoliophyta; Liliopsida;
            Commelinidae; Cyperales; Poaceae.
REFERENCE   1  (bases 1 to 2758)
  AUTHORS   Nakamura,Y. and Yamanouchi,H.
  TITLE     Nucleotide sequence of a cDNA encoding starch branching enzyme or
            Q-enzyme I from rice endosperm
  JOURNAL   Plant Physiol. (1992) In press
  STANDARD  full staff_entry
COMMENT     These data kindly submitted in computer readable form by:
            Yasunori Nakamura
            National Institute of Agrobiological Resources
            2-11 Kannondai, Tsukuba
            Ibaraki 305
            Japan
            Phone:  0298-38-8382
            Fax:    0298-38-8347
FEATURES             Location/Qualifiers
     CDS             30..2492
                     /EC_number="2.4.1.18"
                     /note="a-1,4-glucan, a-1, 4-glucan 6-glucosyltransferase"
                     /product="Q-enzyme(starch branching enzyme)"
                     /codon_start=1
                     /translation="MLCLTSSSSSAPPPLLPSLADRPSPGIAGGGGNVRLSVVSSPRR
                     SWPGKVKTNFSVPATARKNKTMVTVVEEVDHLPIYDLDPKLEEFKDHFNYRIKRYLDQ
                     KCLIEKHEGGLEEFSKGYLKFGINTVDGATIYREWAPAAQEAQLIGEFNNWNGAKHKM
                     EKDKFGIWSIKISHVNGKPAIPHNSKVKFRFRHGGGAWVDRIPAWIRYATFDASKFGA
                     PYDGVHWDPPACERYVFKHPRPPKPDAPRIYEAHVGMSGEEPEVSTYREFADNVLPRI
                     RANNYNTVQLMAIMEHSYYASFGYHVTNFFAVSSRSGTPEDLKYLVDKAHSLGLRVLM
                     DVVHSHASNNVTDGLNGYDVGQNTHESYFHTGDRGYHKLWDSRLFNYANWEVLRFLLS
                     NLRYWMDEFMFDGFRFDGVTSMLYHHHGINKGFTGNYKEYFSLDTDVDAIVYMMLANH
                     LMHKLLPEATIVAEDVSGMPVLCRPVDEGGVGFDFRLAMAIPDRWIDYLKNKEDRKWS
                     MSEIVQTLTNRRYTEKCIAYAESHDQSIVGDKTIAFLLMDKEMYTGMSDLQPASPTIN
                     RGIALQKMIHFITMALGGDGYLNFMGNEFGHPEWIDFPREGNNWSYDKCRRQWSLVDT
                     DHLRYKYMNAFDQAMNALEEEFSFLSSSKQIVSDMNEKDKVIVFERGDLVFVFNFHPN
                     KTYKGYKVGCDLPGKYRVALDSDALVFGGHGRVGHDVDHFTSPEGMPGVPETNFNNRP
                     NSFKVLSPPRTCVAYYRVDEDREELRRGGAVASGKIVTEYIDVEATSGETISGGWKGS
                     EKDDCGKKGMKFVFRSSDEDCK"
BASE COUNT      770 a    565 c    675 g    748 t
ORIGIN
        1 cgcctccacg gccaccgaca tccgccgcaa tgctgtgtct cacctcctct tcctcctccg
       61 cgccgcctcc gctccttccc tctctcgctg atcgaccgag cccgggaatc gcgggcgggg
      121 gtggcaatgt tcgcctgagc gtggtttctt cgccgcgccg gtcgtggcct ggaaaggtca
      181 agaccaattt ctcagttcct gcgactgcgc gaaaaaacaa aaccatggtg actgttgtgg
      241 aggaggtcga ccaccttcct atatatgatc tggaccctaa gttggaggaa ttcaaggatc
      301 acttcaacta taggataaaa agatacctcg accagaaatg cctgattgaa aaacatgagg
      361 ggggccttga agaattttct aaaggctatt tgaagtttgg gattaataca gttgatggtg
      421 ccacaatata tcgtgaatgg gcgcctgctg cacaagaagc acagctcatt ggtgagttca
      481 ataactggaa tggtgcaaaa cacaagatgg agaaggataa atttggcatt tggtcaatca
      541 agatttcaca tgtcaatggg aagcctgcca tccctcacaa ttccaaggtt aaatttcgct
      601 ttaggcatgg gggtggagca tgggttgatc gtattcccgc atggattcgt tatgcaactt
      661 ttgatgcctc taaatttgga gctccatatg atggtgtaca ctgggatcct ccagcctgtg
      721 aaaggtacgt gtttaagcat cctcgacctc caaaacctga tgctccacgc atctatgagg
      781 ctcatgtggg gatgagtggt gaagagccag aagtaagcac atacagagaa tttgcagaca
      841 atgtgttacc acgcatacgg gcaaataact acaacacagt tcagttaatg gcaatcatgg
      901 aacattccta ctatgcttct tttgggtatc acgtgacaaa ttttttcgca gtcagcagca
      961 gatcaggaac accagaggat ctgaaatatc ttgttgacaa ggcacatagt ttaggattac
     1021 gagttctgat ggatgttgtc catagccatg cgagtaataa tgtgaccgat ggtctaaatg
     1081 gctatgacgt tggacaaaac actcatgagt cttattttca tacaggagat aggggctacc
     1141 ataaactctg ggatagtcgt ctgttcaact atgccaattg ggaggtctta agatttcttc
     1201 tttctaattt gagatattgg atggacgaat tcatgtttga tggcttccga tttgatgggg
     1261 ttacatcaat gctataccat caccatggta tcaataaggg atttactgga aactacaagg
     1321 agtatttcag tttggatacc gatgtggatg caattgttta catgatgctc gcaaaccatt
     1381 taatgcataa actcttgccg gaagcaacta ttgttgctga agatgtttcg ggcatgccag
     1441 tgctttgtcg gccagttgat gaaggtggag tagggtttga cttccgcctg gcaatggcca
     1501 ttcctgatag atggattgac tacctgaaga acaaagagga ccgcaaatgg tcaatgagtg
     1561 aaatagtgca aactttgact aacaggagat atacagaaaa atgcattgcc tatgccgaga
     1621 gccatgatca gtccattgtt ggtgacaaga ctatagcatt tctcttgatg gacaaggaaa
     1681 tgtacactgg catgtcagac ttgcagcctg cttcacctac catcaaccgt ggcattgcac
     1741 tccaaaagat gattcacttc attacgatgg cccttggagg tgatggctac ttaaatttta
     1801 tgggcaatga gtttggccat ccagaatgga ttgactttcc aagagaaggc aacaactgga
     1861 gctatgataa atgcagacgt cagtggagcc ttgtcgacac tgatcacctt cgatacaagt
     1921 atatgaatgc atttgatcaa gcaatgaatg cactcgagga ggaattttcc ttcctgtcat
     1981 catcaaagca gattgttagc gacatgaacg agaaagataa ggttattgtc tttgaacgtg
     2041 gagatttggt ttttgttttc aattttcatc ccaacaaaac ttacaagggt tacaaagtcg
     2101 gatgtgactt gcccgggaag tacagagtag ctctggactc tgatgctttg gtctttggtg
     2161 gccatggaag agttggccat gatgtggatc acttcacgtc tcccgaggga atgccaggag
     2221 taccagaaac aaatttcaac aaccgcccta actcattcaa agtcctttcc ccgccccgta
     2281 cctgtgtggc ttactatcgc gttgatgaag atcgtgaaga gctcaggagg ggtggagcag
     2341 ttgcttctgg aaagattgtt acagagtata tcgatgttga agcaacaagt ggggagacta
     2401 tctctggtgg ctggaagggc tccgagaagg acgattgtgg caagaaaggg atgaagtttg
     2461 tgtttcggtc ttctgacgaa gactgcaaat gaagcatcag atttcttgat caggagcaac
     2521 tgttggtgcc cttgtaatct ggagatcctg gcttgccttg gacttggttg tggttcttta
     2581 gcagttgcta tgtacctatc tatgatatga actttatgta tagttcgcct taaagaaaga
     2641 ataagcagtg atgatgtggc cttaaacctg agctgcacaa gcctaatgta aaaataaagt
     2701 ttcaggcttt catccagaat aaaacagctg ttcatttacc aaaaaaaaaa aaaaaaaa
//