From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: pyruvate kinase [Bacillus stearothermophilus, Genomic, 1636 nt]
Date: 27 Jan 93 05:58:06 GMT
Distribution: bionet
Checksum: 43481 5

LOCUS       S118856      1636 bp    DNA             BCT       21-JAN-1993
DEFINITION  pyruvate kinase [Bacillus stearothermophilus, Genomic, 1636 nt]
ACCESSION   S49714 X57859
KEYWORDS    .
SOURCE      Bacillus stearothermophilus
  ORGANISM  Bacillus stearothermophilus
            Unclassified.
REFERENCE   1  (bases 1 to 1636)
  AUTHORS   Walker,D., Chia,W.N. and Muirhead,H.
  TITLE     Key residues in the allosteric transition of Bacillus
            stearothermophilus pyruvate kinase identified by site-directed
            mutagenesis.
  JOURNAL   J. Mol. Biol. 228, 265-276 (1992)
  STANDARD  full automatic
COMMENT     This entry [NCBI gibbsq 118856] was created by the journal scanning
            component of NCBI/GenBank at the National Library of Medicine.
            This sequence comes from Fig. 2.
FEATURES             Location/Qualifiers
     CDS             114..1598
                     /note="mismatches(217[K->Q],370[S->L],447[E->K]).  For the
                     protein sequence (NCBI gibbsq 118859):  Method: conceptual
                     translation with partial peptide sequencing.  This
                     sequence comes from Fig. 2.
                     Author-given protein sequence is in conflict with the
                     conceptual translation"
                     /product="pyruvate kinase"
                     /codon_start=1
                     /translation="MKRKTKIVCTIGPASESVDKLVQLMEAGMNVARLNFSHGDHEEH
                     GRRIANIREAAKRTGRTVAILLDTKGPEIRTHNMENGAIELKEGSKLVISMSEVLGTP
                     EKISVTYPSLIDDVSVGAKILLDDGLISLEVNAVDKQAGEIVTTVLNGGVLKNKKGVN
                     VPGVKVNLPGITEKDRADILFGIRQGIDFIAASFVRRASDVLEIRELLEAHDALHIKI
                     IAKIENEEGVANIDEILEAADGLMVARGDLGVEIPAEEVPLIQKLLIKKCNMLGKPVI
                     TATQMLDSMQRNPRPTRAEASDVANAIFDGTDAVMLSGETAAGQYPVEAVKTMHQIAL
                     RTEQALEHRDILSQRTKESQTTITDAIGQSVAHTASNLDVAAIVTPTVSGKTPQMVAK
                     YRPKAPIIAVTSNEAVSRRLALVWGVYTKEAPHVNTTDEMLDVAVDAAVRSGLVEHGD
                     LVVITAGVPVGETGSTNLMKVHVISDLLAKGQGIGASRRSARPL"
BASE COUNT      418 a    426 c    506 g    286 t
ORIGIN      
        1 gtcgaccatg acatcgcgga ggcgctagcc aacaaacata cgattgatca gcggatgtac
       61 gcattgtcga aagaattgtc catttaagtc ccgaggggaa aggagaagac aagatgaagc
      121 ggaaaacgaa aatcgtctgc acgatcgggc cggcaagcga gagcgtagac aagctcgtgc
      181 aactgatgga agcgggaatg aacgtggcgc gcctaaactt ttcgcacggc gatcatgaag
      241 agcacgggcg gcgcatcgcc aatattcgcg aagcggcgaa gcgaacgggc cgaacggtcg
      301 ccattttgct tgacacgaaa gggccggaaa tccggacgca caatatggaa aacggcgcca
      361 ttgagctcaa ggaagggtca aagctcgtca tttcgatgag cgaagtgctc ggcacgccgg
      421 aaaaaatttc ggtcacctat cccagcttaa tcgatgatgt gtccgtcggc gcgaaaattt
      481 tgcttgatga cgggctcatc agcctggaag tcaacgcggt cgacaagcaa gcgggagaaa
      541 tcgtcacgac cgtgttaaac ggcggcgtgc tgaaaaacaa aaaaggagtc aatgtgccgg
      601 gtgtcaaagt caacttgcca ggcatcaccg agaaagatcg ggctgacatt ttgtttggca
      661 ttcgccaagg gatcgatttc atcgccgcct cgtttgtgcg ccgggcgtcc gatgtgctgg
      721 agatccgcga gctgcttgag gcgcatgacg ccctccatat ccaaattatc gccaaaattg
      781 aaaatgaaga aggcgtcgcc aacattgacg aaattttaga agccgccgac ggcctgatgg
      841 tggcgcgcgg cgatttgggc gtggaaattc cggctgagga agtgccgctc attcaaaaac
      901 tgctcattaa aaagtgcaac atgctcggca agccggtcat tacggcgacg caaatgctcg
      961 attcgatgca gcgcaatccg cgtccgacgc gggcggaggc gagcgacgtc gccaacgcca
     1021 tttttgacgg caccgacgcc gtcatgcttt ccggggaaac ggcggccggc cagtatccgg
     1081 tcgaagcggt gaaaacgatg caccaaatcg cgctccgcac cgaacaggcg cttgagcacc
     1141 gcgacatttt gtcccaacgc acaaaagaaa gccaaacgac gatcaccgac gccatcgggc
     1201 agtcggtcgc ccacaccgcg ctcaacttgg atgtggcggc gatcgtgacg ccgaccgtga
     1261 gcggaaaaac gccgcaaatg gtggcgaagt atcgcccgaa ggcgccgatt attgccgtga
     1321 cgtcaaatga agcggtatcg cgccggctgg cgcttgtgtg gggcgtgtat acgaaagaag
     1381 cgccgcatgt gaacacgacg gacgaaatgc tcgatgtcgc cgtcgatgcg gcggtgcgct
     1441 ccgggttggt gaagcacggt gacttggtcg tcatcacggc tggcgtgccg gtcggcgaaa
     1501 cgggttcgac gaacttaatg aaggtgcacg ttatcagcga tcttctcgcc aaagggcaag
     1561 gcatcggcgc aagtcggcgt tcggcaaggc cgttgtagcg aaaacagcgg aagaagcgcg
     1621 gcaaaaaatg gtcgac
//