From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Gloeocercospora sorghi cyanide hydratase (Cht) gene, complete cds.
Date: 13 Aug 92 10:00:29 GMT
Distribution: bionet
Checksum: 41673     4

LOCUS       GLECYHY      1320 bp ds-DNA             PLN       13-AUG-1992
DEFINITION  Gloeocercospora sorghi cyanide hydratase (Cht) gene, complete cds.
ACCESSION   M99044
KEYWORDS    .
SOURCE      Gloeocercospora sorghi (strain T-442) DNA.
  ORGANISM  Gloeocercospora sorghi
            Eukaryota; Fungi; Eumycota; Deuteromycotina; Ascomycetes;
            Hyphomycetes.
REFERENCE   1  (bases 1 to 1320)
  AUTHORS   Wang,P. and VanEtten,H.D.
  TITLE     Cloning and properties of a cyanide hydratase gene from the
            phytopathogenic fungus Gloeocercospora sorghi
  JOURNAL   Biochem. Biophys. Res. Commun. (1992) In press
  STANDARD  full staff_entry
FEATURES             Location/Qualifiers
     TATA_signal     12..17
                     /gene="Cht"
     mRNA            99..>1260
                     /gene="Cht"
     mRNA            101..>1260
                     /gene="Cht"
     mRNA            103..>1260
                     /gene="Cht"
     CDS             154..1260
                     /EC_number="4.2.1.66"
                     /product="cyanide hydratase"
                     /gene="Cht"
                     /codon_start=1
                     /translation="MPINKYKAAVVTSEPVWENLEGGVVKTIEFINEAGKAGCKLIAF
                     PEVWIPGYPYWMWKVNYLQSLPMLKAYRENSIAMDSSEMRRIRAAARDNQIYVSIGVS
                     EIDHATLYLTQVLISPLGDVINHRRKIKPTHVEKLVYGDGSGDSFEPVTQTEIGRLGQ
                     LNCWENMNPFLKSLAVARGEQIHVAAWPVYPDLSKQVHPDPATNYADPASDLVTPAYA
                     IETGTWVLAPFQRISVEGLKRHTPPGVEPETDATPYNGHARIFRPDGSLYAKPAVDFD
                     GLMYVDIDLNESHLTKALADFAGHYMRPDLIRLLVDTRRKELVTEVGGGDNGGIQSYS
                     TMARLGLDRPLEEEDYRQGTDAGETEKASSNGHA"
BASE COUNT      302 a    424 c    342 g    252 t
ORIGIN
        1 tgagctttac atataaaggg ccacgataca tctgcccttg agttgactgg aacccaaaag
       61 ccatcacaca aacacagtga ctccagcact tcaacgcatt cgcacactcc acgaaccatc
      121 tcgcctcccg aacggccaag cctatcagcc aagatgccca tcaacaagta caaggcggcc
      181 gttgtcacct ccgagcccgt ctgggagaac ctcgaaggag gcgtcgtcaa gaccatcgag
      241 ttcatcaacg aggctggcaa ggcaggatgc aaactcatcg ccttccctga agtttggatc
      301 cctggctacc cctactggat gtggaaggtg aactaccttc agtccctccc catgctcaag
      361 gcctaccgcg agaactcgat cgccatggac tcgtccgaaa tgcgccgcat ccgtgccgca
      421 gcccgcgaca accagatcta cgtttccatc ggagtgtccg agatcgacca tgccaccctc
      481 tacttgaccc aggtcctcat ctcgccactg ggcgacgtca tcaaccaccg ccgcaagatc
      541 aagcctactc atgtcgagaa gctcgtctac ggcgatggct caggcgactc ctttgagcct
      601 gttacccaga ctgagatcgg acgtctcggc cagcttaact gctgggagaa catgaacccc
      661 ttcctcaagt cccttgctgt cgcgcggggc gagcagattc acgtcgccgc ctggcccgtc
      721 tacccagacc tctcgaagca ggttcacccc gatccggcca caaactacgc tgacccggcc
      781 tcggacctcg tcaccccggc gtacgcaatc gagacaggca cctgggtcct cgctcccttc
      841 cagcgcatct ccgtcgaggg gttgaaacga cacactcctc ctggcgtaga gcccgagaca
      901 gacgcgaccc cgtacaatgg ccacgccagg atcttccgcc ccgacggcag cttgtatgcg
      961 aagcctgccg tggacttcga cggtctgatg tatgtcgaca ttgatttgaa tgagtcgcat
     1021 ctgaccaagg cgcttgccga ctttgccggc cactacatga ggcccgactt gatccgtctg
     1081 cttgtggaca ccagacgcaa ggagcttgtt acagaggtcg gcggtggcga caatggcggg
     1141 attcagtcat attcgaccat ggccaggctt ggtcttgatc gcccccttga ggaggaggac
     1201 tatcgtcaag gcacggatgc tggggagact gaaaaagcta gttccaatgg acatgcctaa
     1261 agagggcgtg taggtgaagg gcatagaatc tccactgaaa gcgctactgc aagtaggaaa
//