From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Escherichia coli GDP-mannose pyrophosphorylase (rfbM) gene,
Date: 29 Dec 92 01:27:27 GMT
Distribution: bionet
Checksum: 61049 10

LOCUS       ECOGDPMPP    4278 bp ds-DNA             BCT       08-DEC-1992
DEFINITION  Escherichia coli GDP-mannose pyrophosphorylase (rfbM) gene,
            phosphomannomutase (rfbK) gene, and gluconate-6-phosphate
            dehydrogenase (gnd) gene; complete cds's.
ACCESSION   L04596
KEYWORDS    GDP-mannose pyrophosphorylase; gluconate-6-phosphate dehydrogenase;
            phosphomannomutase.
SOURCE      Escherichia coli (strain VW187) DNA.
  ORGANISM  Escherichia coli
            Prokaryotae; Gram-negative facultatively anaerobic rods;
            Enterobacteriaceae.
REFERENCE   1  (bases 1 to 4278)
  AUTHORS   Marolda,C.L. and Valvano,M.A.
  TITLE     Identification, expression, and DNA sequence of the GDP-mannose
            biosynthesis genes encoded by the LPS rfb region of strain VW187
            (Escherichia coli O7:K1)
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     CDS             98..925
                     /label=orf275
                     /note="unknown function"
                     /codon_start=1
                     /translation="MSDDTPKFSVLMAIYIKDSPLFLSEALQSIYKNTVAPDEVIIIR
                     DGKVTSELNSVIDSWRRYLNIKDFTLEKNMGLGAALNFGLNQCMHDLVIRADSDDINR
                     TNRFECILDFMTKNGDVHILSSWVEEFEFNPGDKGIIKKVPSRNSILKYSKNRSPFNH
                     PAVAFKKCEIMRVGGYGNEYLYEDYALWLKSLANGCNGDNIQQVLVDMRFSKETAKRR
                     GGIKYAISEIKAQYHFYRANYISYQDFIINIITRIFVRLLPTSFRGYIYKKVIRRFL"
     CDS             922..2316
                     /function="converts mannose-1-phosphate into GDP-mannose"
                     /EC_number="2.7.7.13"
                     /standard_name="GDP-mannose pyrophosphorylase"
                     /gene="rfbM"
                     /evidence="EXPERIMENTAL"
                     /label=RfbMEcO7
                     /citation=[1]
                     /product="GDP-mannose pyrophosphorylase"
                     /codon_start=1
                     /translation="MSSPLIPVILSGGNGTRLWPLSREEYPKQFLKLTDSISMLQSTI
                     SRLDSLNTSSPVVICNELHRFIVAEQLRHLNKLDNNIILEPSGRNTAPAICIAALILK
                     MKHPNENPLMLVLPADHSVKKVKTFCNTIKSAIPFAEAGNLVSFGIKPTHPETGYGYI
                     QKGKVLSDSDIYEVSEVRTFVEKPNLKTAESFIEKDEYYWNSGMYLFSVERYLQELSL
                     YRPDIVKVCQETVKNIHYDMDFIRLDDKIFRNCPQESIDYAVMEKTKDAVVATMDIGW
                     NDVGAWSSLWELGKKDSSGNVITGDIVCHETENSYIYTESGLVATIGIQDLVIIHTKD
                     SLLVSRRDSVQNVKNIVQHLDLSGRKEHKEHREVFKSWGRCDSIDSSEKYHYQVKRIT
                     VNPSENYRCNYIITVRNIGVVVMGIAKLTVAEEIKILKENESVYIPAGIKHSLKILDN
                     TTCVNRSLDRFLSC"
     CDS             2378..3739
                     /EC_number="5.4.2.8"
                     /standard_name="phosphomannomutase"
                     /gene="rfbK"
                     /evidence="EXPERIMENTAL"
                     /citation=[1]
                     /product="phosphomannomutase"
                     /codon_start=1
                     /translation="MLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLGGD
                     VRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPMDY
                     NGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLFGY
                     INVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIPNP
                     LLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLEKN
                     PGAKIIHDPRLSWNTVDVVTAAGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHYFRD
                     FAYCDTGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAHPVEAINRVEQ
                     HFSRDAGGGSHRWHQHDLCRLAALTCASSNTEPVVRLNVESRGDVPLMEEKTKLILEL
                     LNK"
     CDS             3903..>4278
                     /EC_number="1.1.1.44"
                     /standard_name="6-phosphogluconate dehydrogenase"
                     /gene="gnd"
                     /evidence="EXPERIMENTAL"
                     /label=GndEcO7
                     /citation=[1]
                     /product="gluconate-6-phosphate dehydrogenase"
                     /codon_start=1
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKQEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFYRY"
BASE COUNT     1287 a    822 c    965 g   1204 t
ORIGIN      
        1 aagttgaccc aaaattgttt gaagatgaat tatcacgctt ttctactaat aaacaaataa
       61 atactttgcc agcacatgtt taaggaaata tgtaaagatg tcagatgaca ctccaaagtt
      121 ttctgttttg atggctatat acatcaagga ttctccccta tttctctctg aggctttaca
      181 atcaatttat aaaaatacgg ttgccccaga tgaagttatt attattcgtg atggtaaggt
      241 cacatctgaa ctcaattctg ttattgatag ttggagaaga tatttaaata taaaagattt
      301 cacacttgaa aaaaatatgg ggttgggggc agcgttaaat tttgggttga atcaatgtat
      361 gcatgattta gtaatacgtg ctgattctga tgatataaat agaacaaatc gattcgaatg
      421 tatacttgat tttatgacta agaatggtga tgttcatatt cttagctctt gggttgaaga
      481 gttcgaattc aatccaggag ataagggcat aataaaaaaa gtaccaagtc ggaattctat
      541 acttaaatat tcaaaaaaca gatctccttt taatcaccct gcagttgcat ttaaaaagtg
      601 tgaaataatg cgcgtcggcg gttatggaaa tgagtactta tatgaagatt atgccctttg
      661 gttgaaatct ttagctaatg gatgtaatgg tgataatatt caacaagttt tagttgatat
      721 gagattttca aaggaaacag caaaaagacg aggtggaata aaatatgcca tttcagaaat
      781 taaagctcag tatcattttt atcgtgcaaa ttatatatca tatcaagact ttataataaa
      841 tattatcaca aggatatttg ttagacttct tcccactagt tttcgtggct atatatataa
      901 aaaagttatc aggagatttt tatgagctca cctcttattc cggttatatt aagtggtggt
      961 aatggtacta ggttatggcc actatctaga gaggaatatc ctaagcagtt tttaaaacta
     1021 accgactcaa tatcaatgct gcaatcaaca atatctcggt tagactcatt aaatacttcc
     1081 tctccagttg taatatgcaa tgaattacac agatttattg ttgcagaaca actcaggcac
     1141 ttaaataaat tagataataa tattatttta gaaccatctg gtcgcaatac tgcacctgct
     1201 atttgtattg ctgctttaat tttaaaaatg aagcatccaa atgaaaatcc acttatgctc
     1261 gttcttccag ccgatcactc cgtaaaaaaa gtcaaaactt tttgtaatac aataaaaagt
     1321 gctattccct tcgctgaagc tggtaatttg gtttcttttg gtattaaacc tactcatcct
     1381 gagacggggt atggatatat acaaaaaggc aaagtgttat ctgattctga tatatatgag
     1441 gtcagtgaag ttagaacttt tgttgaaaag cctaatctta aaacagcaga aagctttata
     1501 gaaaaagatg agtattattg gaatagtggt atgtatttgt tcagtgttga acgttactta
     1561 caagagttat cattataccg accagacata gttaaagtat gccaggaaac tgttaaaaat
     1621 attcattatg atatggattt tattagattg gacgataaaa tatttcggaa ctgtccacag
     1681 gagtctattg attatgctgt aatggagaaa acaaaggatg ctgtagttgc tacaatggat
     1741 atcggttgga atgatgtagg agcatggtct tcgctttggg aattagggaa aaaagactcc
     1801 tctggtaatg ttatcacggg agacatcgtt tgccacgaga cagaaaatag ttatatttat
     1861 actgagtctg gattggtagc aactattggt attcaagatc ttgttattat tcatactaaa
     1921 gattcattac tggtttccag acgcgattca gtacaaaatg taaaaaatat tgttcagcat
     1981 cttgatttgt caggacgtaa agaacataaa gaacataggg aagtattcaa gtcatgggga
     2041 cgatgtgact ccatagatag tagcgaaaag taccactatc aggtcaaacg aataacagtt
     2101 aatccaagtg aaaattatcg ttgcaattac atcatcaccg tgcggaacat tggggttgtt
     2161 gtaatgggga ttgctaaact tacagttgca gaagaaataa aaattttaaa agagaatgag
     2221 tctgtatata ttcctgcagg tattaagcat agtttgaaaa tattggacaa taccacttgt
     2281 gttaatagaa gtttggaccg gttcttatct tgctgatgat gatatccttc gatttgaaga
     2341 ttgatatggt agagcttaga aatagagagt cttaataatg ctaacttgct ttaaagctta
     2401 tgatattcgc gggaaactag gcgaagaact gaatgaagat atcgcctggc gcattgggcg
     2461 tgcctatggc gaatttctca aaccgaaaac cattgtgtta ggcggtgatg tccgcctcac
     2521 cagcgaaacc ttaaaactgg cgctggcgaa aggtttacag gatgcgggcg tcgatgtgct
     2581 ggatatcggt atgtccggca ccgaagagat ctatttcgcc acgttccatc tcggcgtgga
     2641 tggtggcatt gaagttaccg ccagccataa tccgatggat tataacggca tgaaactcgt
     2701 gcgcgagggg gctcgcccga tcagcgggga taccggactg cgcgatgtcc agcgtctggc
     2761 agaagccaac gacttccctc ccgttgatga aaccaaacgc ggtcgttatc agcaaatcaa
     2821 tctgcgtgac gcttacgttg atcacctgtt cggttatatc aacgtcaaaa acctcacgcc
     2881 gctcaagctg gtgattaact ccgggaatgg cgcggcgggg ccggtggtgg acgccattga
     2941 agcccgcttt aaagccctcg gcgcaccggt ggaattaatc aaagtacaca acacgccgga
     3001 cggcaatttc cccaacggta ttcctaaccc gttgctgccg gaatgccgcg acgacacccg
     3061 taatgcggtc atcaaacacg gcgcggatat gggcattgcc tttgatggcg attttgaccg
     3121 ctgtttcctg tttgacgaaa aagggcagtt tatcgagggc tactacattg tcggcctgct
     3181 ggcagaagca ttcctcgaaa aaaatcccgg cgcgaagatc atccacgatc cgcgtctctc
     3241 ctggaacacc gttgatgtgg tgaccgccgc gggcaccccg gtaatgtcga aaaccggaca
     3301 cgcctttatt aaagaacgta tgcgcaagga agacgccatc tacggtggcg aaatgagcgc
     3361 ccaccactat ttccgtgatt tcgcttactg cgacacgggc atgatcccgt ggctgctggt
     3421 ggccgaactg gtgtgtctga aaggaaaaac gctgggcgaa ctggtgcgcg accggatggc
     3481 ggcgtttccg gcaagcggtg agatcaacag caaactggcg caccccgttg aggcgattaa
     3541 tcgcgtcgaa cagcatttta gccgcgacgc tggcggtgga tcgcaccgat ggcatcagca
     3601 tgacctttgc cgactggcgg ctttaacgtg cgcctcctcc aacaccgaac cggtggtgcg
     3661 gttgaatgtg gaatcgcgcg gtgatgtacc gctgatggaa gaaaagacaa aacttatcct
     3721 tgagttactg aacaagtaat tcagtaattt catataaatg gtttttaaaa aacggaaaag
     3781 atgagatatc cggtgtggta tagccaaggt aatgctattc agtatctcta tgagtgagtt
     3841 aacatctata ccacatttaa gccgcacact tcgcggtgac caccccctga caggagtaaa
     3901 caatgtcaaa gcaacagatc ggcgtcgtcg gtatggcagt gatggggcgc aacctcgcgc
     3961 tcaacatcga aagtcgtggt tataccgtct ctattttcaa ccgttcccgt gaaaagcagg
     4021 aagaagtgat tgccgaaaat ccaggcaaga aactggttcc ttactatacg gtgaaagagt
     4081 ttgttgaatc tctggaaacg cctcgtcgca tcctgttaat ggtgaaagca ggtgcaggca
     4141 cggatgctgc tattgattcc ctcaagccat acctcgataa aggtgacatc atcattgatg
     4201 gtggtaatac cttcttccag gacaccattc gtcgtaaccg tgagctttct gccgaaggct
     4261 ttaattttta tcggtacc
//