From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Brucella abortus immunoreactive stress response protein gene,
Date: 27 Jan 93 05:46:57 GMT
Distribution: bionet
Checksum: 34315 6

LOCUS       BRUHTRAH     1905 bp ds-DNA             BCT       26-JAN-1993
DEFINITION  Brucella abortus immunoreactive stress response protein gene,
            complete cds.
ACCESSION   L09274
KEYWORDS    homologue; immunoreactive stress response protein.
SOURCE      Brucella abortus (strain 2308) (library: Sau3A/BamHI library of
            M.Roop) DNA.
  ORGANISM  Brucella abortus
            Prokaryotae; Gracilicutes; Scotobacteria; Neisseriaceae.
REFERENCE   1  (bases 1 to 1905)
  AUTHORS   Roop,R.M.II., Fletcher,T.W., Sriranganathan,N.M., Boyle,S.M. and
            Shurig,G.G.
  TITLE     Cloning and expression of the gene encoding an immunoreactive
            Brucella abortus HtrA stress response protein homologue
  JOURNAL   Unpublished (1993)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     promoter        183..213
                     /function="sigma E type promoter"
                     /note="This region of nucleotide sequence shows a high
                     level ofhomology with the sigma type E promoters
                     previouslydescribed for the HtrA genes of E. coli and S.
                     typhimuriumand the rpoH gene of E. coli; putative"
     RBS             322..326
     sig_peptide     333..407
                     /codon_start=1
     mat_peptide     408..1832
                     /product="immunoreactive stress response protein"
                     /evidence="EXPERIMENTAL"
                     /codon_start=1
     CDS             333..1835
                     /evidence="EXPERIMENTAL"
                     /note="This 45 kD protein precursor is an immunoreactive
                     protein, the deduced amino acid sequence shows 40.5% and
                     43.2% identity with the Escherichia coli and Salmonella
                     typhimurium HtrA (DegP) stress respon"
                     /product="immunoreactive stress response protein"
                     /codon_start=1
                     /translation="MSRARISNYRKGVAAVALSASLAGAFVVTGPLGALNEARAEAVH
                     VTPPPQAGFADLVEKVRPAVVSVRVKKDVQETSNRGPQFFGPPGFDQLPDGHPLKRFF
                     RDFGMEPRGDSRSDNRRGKANKPRPGHERPVAQGSGFVISEDGYVVTNNHVVSDGDAY
                     TVVLDDGTELDAKLIGADPRTDLAVLKINAPKRKFVYSPLATIIRCDVGDWVVAVGNP
                     FGLGGTVTSGIVSARGADIGAGPYDDFIQIDAAVNKGNSGGPAFDLSGEVIGINTAIF
                     SPSGGTVGIAFAIPSSTAKQVVDQLIKKGSVERGWIGVQIQPVTKDIAASLGLAEEKG
                     AIVASPQDDGPAAKAGIKAGDVITAVNGETVQDPRDLARKVANIAPGEKAALTVWRKN
                     KAEEINVTIAAMPNDKGKSGSQSNDNDGGQGETLDSYGLTVVPSEDGKGVVVTDVDPD
                     SDAADRGIRSGDVIVSVNNQTVKTAGDINKAITAAEKSGRNGAAAAIAEQ"
BASE COUNT      393 a    558 c    564 g    390 t
ORIGIN      
        1 aattcgttct tctcaaaccc cgtttcaacg cgcagacggc gctgctttgg ggctttcctg
       61 ttattattct cctgattggc ggcggcgcgc ttcttgttgc tttccggcgt gcggcgcgct
      121 gctggcacag ccccacagcc ccttccgaaa gcgaaaaagc ggaactttcc cgcctgctcg
      181 acggaaaata ggccaacaaa acctctggaa accttacgaa actttcatct tgcggaaatt
      241 gaacggtaag gtagctctaa ctatcttgct ctcatagaaa attacgaggc cagaaattcc
      301 tggcccggat tactgctcgc aaggagaaac agatgtccag agccaggatt tccaactatc
      361 gcaaaggcgt tgccgccgtc gcgctttccg caagccttgc cggcgcattc gtcgtaaccg
      421 gcccgcttgg tgcgctgaac gaagcccgcg ccgaagcggt gcatgtgacg ccgccgccgc
      481 aggctggctt tgccgatctg gtggaaaagg ttcgccctgc ggtcgtcagc gtccgcgtca
      541 agaaggacgt gcaggagact tccaaccgtg gtccgcagtt cttcggccct cccggcttcg
      601 accagttgcc ggacggtcat ccgctgaagc gatttttccg tgattttggt atggagccgc
      661 gtggcgattc acgctccgat aaccgtcgcg gcaaggccaa caagccgcgc ccgggccatg
      721 aacgccctgt tgcacaagga tcgggcttcg tcatttccga agatggctat gtcgttacca
      781 acaaccatgt cgtttccgat ggcgatgcct ataccgtcgt gctggacgac ggcaccgaac
      841 ttgatgccaa gctgattggt gctgatccgc gcacggacct ggccgtgttg aaaatcaacg
      901 ctccgaagcg caagttcgtc tattcgcctt tggcgacgat aataaggtgc gacgtcggtg
      961 attgggttgt cgcagttggc aatccgttcg gccttggtgg cacggtgact tccggtatcg
     1021 tttcggcccg tggagccgac atcggcgcag gcccctatga cgatttcatc cagattgatg
     1081 ccgccgtgaa caagggcaat tccggtggtc cggctttcga cctttccggc gaagtcatcg
     1141 gcatcaacac cgccatcttc tcgccatctg gcggcacggt gggcattgct tttgcaattc
     1201 cgtcctccac cgccaagcag gtcgtggacc agctcatcaa gaagggttcc gtcgagcgcg
     1261 gctggatcgg cgtccagata cagcctgtga ccaaggatat cgccgcttcg ctcggtcttg
     1321 ccgaggaaaa gggtgcgatc gtggcctcgc cgcaggatga tggcccggct gccaaggctg
     1381 gcatcaaggc gggcgatgtg attacggctg tcaatggcga aacggttcag gacccgcgtg
     1441 acctggcgcg caaggttgcc aatatcgccc ctggtgaaaa ggcggccctg accgtctggc
     1501 gcaagaacaa ggccgaggaa atcaacgtca cgattgctgc catgcccaat gacaagggga
     1561 agagcggcag ccagtccaac gacaatgacg gcggacaggg tgaaacgctc gactcctacg
     1621 gcttgaccgt cgttccgtct gaagacggta agggcgtggt cgttaccgat gtggacccgg
     1681 acagcgatgc ggctgaccgt ggcatccgct ccggcgatgt gatcgtgagc gtcaacaacc
     1741 agacggtaaa gaccgctggc gatatcaaca aggccatcac ggcagccgaa aagtcaggcc
     1801 gtaacggagc tgctgctgca attgcagagc aatgatcaga gccgcttcgt ggcgctgccg
     1861 atcaatcagg aatgatcggc gcctcgcagg gaaactgatc gaatt
//