From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Trypanosoma brucei variant surface glycoprotein (MVAT-RX2) gene, 5'
Date: 5 Nov 92 23:13:42 GMT
Distribution: bionet
Checksum: 33014 7

LOCUS       TRBVSGM      3475 bp ds-DNA             INV       14-OCT-1992
DEFINITION  Trypanosoma brucei variant surface glycoprotein (MVAT-RX2) gene, 5'
            end.
ACCESSION   L04971
KEYWORDS    alternative splicing; gene duplication;
            metacyclic variable surface glycoprotein; spliced leader;
            variant surface glycoprotein.
SOURCE      Trypanosoma brucei (individual_isolate LVH/75/USAMRU-K/18,
            sub_species rhodesiense) bloodstream form DNA.
  ORGANISM  Trypanosoma brucei
            Eukaryota; Animalia; Protozoa; Sarcomastigophora; Mastigophora;
            Kinetoplastida; Trypanosomatina; Trypanosomatidae.
REFERENCE   1  (bases 1 to 3475)
  AUTHORS   Lu,Y., Hall,T., Gay,L.S. and Donelson,J.E.
  TITLE     Point mutations are associated with a gene duplication leading to
            the bloodstream re-expression of a trypanosome metacyclic VSG
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     misc_recomb     144
                     /note="upstream recombination site for gene duplication"
                     /gene="MVAT5-RX2"
     misc_recomb     1080
                     /note="alternate spliced leader addition site"
                     /gene="MVAT5-RX2"
     misc_recomb     1096
                     /note="alternate spliced leader addition site"
                     /gene="MVAT5-RX2"
     polyA_site      2727
                     /gene="MVAT5-RX2"
     CDS             1106..2660
                     /partial
                     /gene="MVAT5-RX2"
                     /product="variant surface glycoprotein"
                     /codon_start=1
                     /translation="MIGKAFIILSLLNELPSPTAAQAAQGGALGKDVWLPLAKFTATA
                     AKIPGRAAKLLQDRSAQIVNLMKLQVQADICLNKAASEVSALGWQALAVAIAADIGSL
                     QSLQQQRSEEAIAAAAAAEFARGHAAEFFKVAAAVQSAANSGCLTTNNKGGAAGSVIN
                     GFSTLGTAEQPAIGATSTAHVGDDITAITTTGFSDLAATDGIRTDSLTADTNCVLFKG
                     GSAGPLTTANFGQSIPFAGGYLTRNPTANTASSADGTDFVTNPGDGKIAGIKVYRDAH
                     AAAAKIRTAATFGSSFTDFKKLDQAKKSVHLRTAVKNIILGKPDGSVADLSDEIDTKI
                     NQVFGEDQATFHSRFWDQLTKVKVEKAASGQDETTLDAITSFAALSRARTYYSTKVIK
                     GLRDKISSLEIKNSKTEVKVTDADCNKHQSKDKCAAPCKWNENTTDINKKCSLDPVKA
                     TEQQAAQTAGAGEGAAGTTTDKCKDKKKDDCKSPDCKWEGETCKDSSILLNKQFALMG
                     FCSLCGLAFL"
BASE COUNT     1021 a    776 c    731 g    947 t
ORIGIN      
        1 gcgaaagact gcactcaatg ctcgagcatt gctttgggca ttgataatgt acgaaaacgt
       61 tcattggcaa aaaaaaggct gagatacatg gagtattttt ctatacagca tttttcgttg
      121 aatatgacaa ttgaccaagt ggcgagtaat aaagatcttg tcgttaagta aaacaatgtt
      181 cgttgacttt caatcgcact gtttccatct tatgctctca tccgttggtt agttgattag
      241 aaggagttaa ggaaagcgga ttcaataaat aggtatatgc aattacataa ctagaatgtt
      301 tctggaaata ttgcctccac ttttaaagat acatttagtg acattaaaaa tacggttaca
      361 tagatccaat aatactggct tcgcgatcgc agaaaaagaa attaaacagt attcccctaa
      421 gaaaacattt acaacaagtc gtgcatatac agaacgattg ccgcgcttcg gggcgtgccg
      481 ttcgctaaag gggaagcgtt acaatgcttg acgcgctata tggcagcgat ttgattgtta
      541 aactttttct aagagaatgt cgcggctgca gttcatcatt cagcgaaaag aatgaccatc
      601 cgtaaaaccc agagtcaagg gcatataata taataatgcc gcggcgtgca ctggacttag
      661 tgagtgacag agagttaggc actttcaggt gaacagatcg acatagagaa gaataaaaac
      721 catgtttcat tcaaaatgtg ataggcactg ctgcacaaat gctggctact gtagctttta
      781 acatttgtca gttctgaacg cggaccattc ggttgctgcg acggccgcca ttacgtgatt
      841 gaatgtgttt tttaaaattt agtggcagaa tgaagcacaa aaaacataga gaaaaggttt
      901 gattgactaa tagcaaataa agataagagc gatgtaaacg aataaggctt gaaatgtgga
      961 gtttaaaaca aaattcaacc ggcgccttcg aactggctat tataagcatt tccttccttt
     1021 cgcgacgcaa ccagttcaaa tctttctata acaactgaaa caacaactct ttgccaaagc
     1081 agaagcaaca ctgagaactc cacagatgat aggaaaagcc tttattattt tatctttact
     1141 taacgagctg ccaagcccga cggcagcaca agcggcacag ggtggtgccc tcggaaaaga
     1201 cgtatggcta cctctcgcta aattcacggc gacggccgcg aaaatcccag gcagggcggc
     1261 aaagctgctt caagacaggt cggcccaaat agttaacctt atgaaactcc aagttcaggc
     1321 agacatatgc ctcaacaaag cagcgtcaga ggtgagcgca cttgggtggc aggcgctcgc
     1381 tgttgcaata gcagcagaca tcggcagcct gcaaagcttg caacagcaga ggagtgaaga
     1441 ggcaatagcg gccgcggcag ctgccgaatt cgctcggggc cacgcagcgg aattcttcaa
     1501 agtagctgcg gcagtccaaa gcgccgccaa tagcggctgc ctgacaacaa acaataaagg
     1561 tggcgcagcc ggcagcgtga taaacggatt ctcgacactc ggcaccgcgg agcagccagc
     1621 aatcggtgct acatcgacgg ctcacgtcgg cgacgacata acggcgataa caacaacagg
     1681 gttcagcgac ctagcagcaa cagacggcat acgcaccgac tcactaacag cggacacaaa
     1741 ctgcgttctt ttcaagggag gcagcgctgg accgctaacg acagcaaact tcggccaatc
     1801 gatccctttc gcaggcggct atctaacaag gaacccgaca gccaacacag ccagcagcgc
     1861 cgacggtacg gactttgtaa ccaaccccgg cgacggcaag atagcaggca taaaagtcta
     1921 tagggacgcc cacgccgccg cagcgaaaat acgcacagca gcaaccttcg gctcgagctt
     1981 caccgacttc aagaagctag atcaggctaa gaagtcagtc catttgcgca cagcagtaaa
     2041 aaacataatt ctcggcaaac ctgacggatc cgtagccgac ctttccgacg agatagacac
     2101 aaagataaac caggtattcg gcgaagacca agcaacattc cacagcaggt tttgggatca
     2161 actaacaaaa gtaaaagtgg aaaaggcggc gagtgggcaa gacgaaacga ccctcgatgc
     2221 aatcacttct tttgcagcct taagccgagc tcggacttat tactccacga aagtgattaa
     2281 aggtttgaga gataagatat cctcactaga aattaaaaat tccaaaacgg aagttaaagt
     2341 cactgacgcc gactgcaaca aacaccaatc aaaagacaaa tgcgcagccc catgcaaatg
     2401 gaacgagaat accactgaca taaacaaaaa atgctcatta gatcccgtaa aagcgacaga
     2461 acagcaagca gcccagacag caggagcagg agaaggagct gcaggaacaa caacagataa
     2521 atgcaaagat aagaaaaagg atgactgcaa atctccggac tgcaaatggg agggtgaaac
     2581 ttgcaaagat tcctctattc tcctaaacaa acaattcgcc ctaatgggtt tctgcagcct
     2641 ttgtggcctt gcttttttaa ttttttcccc cctctttttc ttaaagaatt tttgctactt
     2701 taaaaacttc tgatatattt taacacctaa acccaaccga gacttttttt gagctgtttt
     2761 tcgtaagttg actgtctgat tctgtaaaat ttttctggaa actaaaattt ttttttcttc
     2821 ttttcctgtt ttttttttgt ggagtttttg taggtaggaa tgggggggtt aggtaggcta
     2881 ggattaggct taggattagg attagactta gggttactcc gttacttaat tttttctctc
     2941 ttcctgctaa ttaatttttc attccaattt ttttttgtct tttatcaatg cggacgcctc
     3001 ctttttattg ttttttattg cgatttgaat tttaattttt taattttttt cacttttttc
     3061 aatttttttt ttaattgcta atttttttaa tgatgatgca gcggctgttt tgttatgcag
     3121 accctaaccc tcctcccccc cgcccgcacc acccccgcgc acctccaatt ttttaatttt
     3181 ttaaaaatct tttcttttaa tttttttttc agattttcac gattttggtc tgcgcgaatt
     3241 ttcttttttt ttttttccgt tttttaactt ttaaaatttt ctttagccat gcagcggcga
     3301 tcttgttacg cggaccctaa ccctcctccc ggcccgggcc cggccaccac acacgcacct
     3361 tttttttcat tttaatattt ttatcgccat cacatttcgt ttttttaatt ttttttactt
     3421 ttcggttttc gggtttttga attttttttt agggccaggt ttttttttaa agctt
//