From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Clostridium acetobutylicum acetoacetate decarboxylase,
Date: 7 Jan 93 05:45:58 GMT
Distribution: bionet
Checksum: 10169 6

LOCUS       CLOACEBUT    2527 bp ds-DNA             BCT       06-JAN-1993
DEFINITION  Clostridium acetobutylicum acetoacetate decarboxylase,
            acetoacetyl-coenzyme A:acetate/butyrate:coenzyme A-transferase
            subunit, complete cds.
ACCESSION   M93363
KEYWORDS    acetoacetate decarboxylase;
            acetoacetyl-CoA:acetate/butyrate:CoA-transferase.
SOURCE      Clostridium acetobutylicum (library: EMBL3, ATCC 824) DNA.
  ORGANISM  Clostridium acetobutylicum
            Prokaryotae; Firmicutes; Endospore-forming rods and cocci;
            Bacillaceae.
REFERENCE   1  (bases 1 to 2527)
  AUTHORS   Petersen,D.J. and Bennett,G.N.
  TITLE     Characterization of the acetone production pathway genes from
            Clostridium acetobutylicum ATCC 824
  JOURNAL   Thesis (1991) Biochemistry and Cell Biology, Rice University
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     CDS             241..975
                     /product="acetoacetate decarboxylase"
                     /codon_start=1
                     /translation="MLKDEVIKQISTPLTSPAFPRGPYKFHNREYFNIVYRTDMDALR
                     KVVPEPLEIDEPLVRFEIMAMHDTSGLGCYTESGQAIPVSCNGVKGDYLHMMYLDNEP
                     AIAVGRELSAYPKKLGYPKLFVDSDTLVGTLDYGKLRVATATMGYKHKALDANEAKDQ
                     ICRPNYMLKIIPNYDGSPRICELINAKITDVTVHEAWTGPTRLQLFDHAMAPLNDLPV
                     KEIVSSSHILADIILPRAEVIYDYLK"
     CDS             complement(1041..1706)
                     /product="acetoacetyl-CoA:acetate/butyrate:CoA-transferase
                     subunit"
                     /codon_start=1
                     /translation="MINDKNLAKEIIAKRVARELKNGQLVNLGVGLPTMVADYIPKNF
                     KITFQSENGIVGMGASPKINEADKDVVNAGGDYTTVLPDGTFFDSSVSFSLIRGGHVD
                     VTVLGALQVDEKGNIANWIVPGKMLSGMGGAMDLVNGAKKVIIAMRHTNKGQPKILKK
                     CTLPLTAKSQANLIVTELGVIEVINDGLLLTEINKNTTIDEIRSLTAADLLISNELRP
                     MAV"
     CDS             complement(1734..2363)
                     /product="acetoacetyl-CoA:acetate/butyrate:CoA-transferase
                     subunit"
                     /codon_start=1
                     /translation="MNSKIIRFENLRSFFKDGMTIMIGGFLNCGTPTKLIDFLVNLNI
                     KNLTIISNDTCYPNTGIGKLISNNQVKKLIASYIGSNPDTGKKLFNNELEVELSPQGT
                     LVERIRAGGSGLGGVLTKTGLGTLIEKGKKKISINGTEYLLELPLTADIALIKGSIVD
                     EAGNTFYKGTTKNFNPYMAMAAKTVIVEAENLVSCEKLEKEKAMTPEFL"
BASE COUNT      800 a    418 c    412 g    897 t
ORIGIN      
        1 gaattcataa aaacacctcc acataagttt atataaatct attatgcaga attttttagg
       61 aagtgtactt ttattttcga aagctagtag aaaaaattaa tatatggtgt aaaaatttac
      121 ttaaaaaaac aatatgtgtt ataatgtaaa tataaataaa taggactaga ggcgatttat
      181 aatgtaagat aaagtatgtt agaaaagcta aacattatta aatttaggaa ggtgactttt
      241 atgttaaagg atgaagtaat taaacaaatt agcacgccat taacttcgcc tgcatttcct
      301 agaggaccct ataaatttca taatcgtgag tattttaaca ttgtatatcg tacagatatg
      361 gatgctcttc gtaaagttgt gccagagcct ttagaaattg atgagccctt agtcaggttt
      421 gaaattatgg caatgcatga tacgagtgga cttggttgtt atacagaaag cggacaggct
      481 attcccgtaa gctgtaatgg agttaaggga gattatcttc atatgatgta tttagataat
      541 gagcctgcaa ttgcagtagg aagggaatta agtgcatatc ctaaaaagct cgggtatcca
      601 aagctttttg tggattcaga tactttagta ggaactttag actatggaaa acttagagtt
      661 gcgacagcta caatggggta caaacataaa gccttagatg ctaatgaagc aaaggatcaa
      721 atttgtcgcc ctaattatat gttgaaaata atacccaatt atgatggaag ccctaggata
      781 tgtgagctta taaatgcgaa aatcacagat gttaccgtac atgaagcttg gacaggacca
      841 actcgactgc agttatttga tcacgctatg gcgccactta atgatttgcc agtaaaagag
      901 attgtttcta gctctcacat tcttgcagat ataatattgc ctagagctga agttatatat
      961 gattatctta agtaataaaa ataagagtta ccttaaatgg taactcttat ttttttaata
     1021 ttgtttcata gtatttcttt ctaaacagcc atgggtctaa gttcattgga tatgagtaaa
     1081 tctgcagcag ttaaagacct tatttcatca atggttgtgt ttttattaat ttcagtgaga
     1141 agtaaaccat cattaataac ctcaattact ccaagttctg ttacaattag atttgcttga
     1201 gactttgccg tgaggggaag tgtacatttt tttaaaattt taggttgacc tttatttgta
     1261 tgtctcattg caattattac tttcttagct ccatttacta aatccatagc tccacccata
     1321 ccagagagca tttttccagg aacaatccaa ttggctatat tacccttttc atctacctgg
     1381 agagccccta aaacagtaac atctacgtga ccaccacgga ttagtgaaaa cgaaactgag
     1441 ctatcgaaaa atgtgccgtc aggaagtact gttgtatagt ctcctcctgc atttactaca
     1501 tctttatctg cctcatttat tttaggacta gcgcccattc caactattcc gttttctgat
     1561 tggaaagtaa ttttgaaatt ttttggtata taatctgcaa ccatggtagg aagacctaca
     1621 cctaagttta caagttgacc attttttaat tctcttgcaa ctcttttggc tattatttct
     1681 ttcgctaggt ttttatcatt aatcatttta tgcaggctcc tttactatat aatttataag
     1741 aactccgggg tcattgcttt ttccttttct agtttttcac agctaactaa attttcagct
     1801 tcaactatta cggttttagc tgccattgcc atatagggat taaagttttt agtagtacct
     1861 ttatagaagg tgtttccggc ctcatctaca atactacctt taattaatgc tatatcggct
     1921 gtaagaggta gctctaacaa atattccgtt ccatttatag atattttttt ctttcctttt
     1981 tcaatcaaag ttcctaaacc tgttttagtt agtacaccac ctaagccaga tccgcctgca
     2041 cgtattcttt ccactagagt tccttgggga gagagctcta cttcaagttc attattaaaa
     2101 agttttttgc cagtatcagg gttgctgcct atatatgaag caataagctt ttttacttga
     2161 ttatttgata ttaacttacc aatacctgta ttaggataac atgtatcatt acttataatc
     2221 gttaaattct ttatatttaa attaactaaa aaatcaatta atttggttgg agtgccacag
     2281 tttaaaaaac ctccaatcat aattgtcatc ccatctttaa agaatgacct taaattttca
     2341 aatctaatta ttttagagtt cattttaatc cctcctttta aattctttat taaaattata
     2401 aaaagtagtt gaaatatgaa ggtttaaggt tgttttttaa aacaaaattt atatacattt
     2461 cttttatctc acttgtaaga ggaaatctag gattagcgcc tgtgcattga tcatctagtg
     2521 caagttc
//