From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Entamoeba histolytica 170 kDa surface antigen.
Date: 27 Aug 92 10:00:18 GMT
Distribution: bionet
Checksum: 29508     7

LOCUS       ENHSAL       3635 bp ds-DNA             INV       27-AUG-1992
DEFINITION  Entamoeba histolytica 170 kDa surface antigen.
ACCESSION   L00636
KEYWORDS    170 kDa surface antigen; 170 kDa surface lectin.
SOURCE      Entamoeba histolytica (strain HM1:IMSS) trophozoite DNA.
  ORGANISM  Entamoeba histolytica
            Eukaryota; Animalia; Protozoa; Sarcomastigophora; Sarcodina;
            Rhizopoda; Lobosa; Gymnamoeba; Schizopyrenida; Tubulina;
            Entamoebidae.
REFERENCE   1  (sites)
  AUTHORS   Tannich,E., Ebert,F. and Horstmann,R.D.
  TITLE     Primary structure of the 170-kDa surface lectin of pathogenic
            Entamoeba histolytica
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 88, 1849-1853 (1991)
  STANDARD  full staff_review
REFERENCE   2  (bases 1 to 3635)
  AUTHORS   Zhang,Y., Li,E., Jackson,T.F., Zhang,T., Gathiram,V. and
            Stanley,S.L.Jr.
  TITLE     Use of a recombinant 170 kDa surface antigen of Entamoeba
            histolytica in serodiagnosis of amebiasis and identification of
            immunodominant domains of the native molecule
  JOURNAL   Unpublished (1992)
  STANDARD  full staff_entry
FEATURES             Location/Qualifiers
     primer_bind     1..30
                     /standard_name="PCR primer"
                     /note="putative"
     misc_feature    968..988
                     /note="This region, along with regions 1321-1347,
                     1679-1692,2321-2341, and 2616-2646, were derived from
                     citation 2, andwere the primers used for sequencing."
                     /citation=2
                     /note="primer sequence derived from citation 2"
                     /note="putative"
                     /citation=[1]
     misc_feature    3100..3615
                     /note="this sequence was derived from a second clone"
                     /note="putative"
     primer_bind     complement(3616..3634)
                     /standard_name="PCR primer"
                     /note="putative"
BASE COUNT     1428 a    481 c    642 g   1084 t
ORIGIN
        1 gtaggcctga taaacttaat gaattttcag cagatattga ttattatgac cttggtatta
       61 tgtcccgtgg aaagaatgca ggttcatggt atcattctta tgaacatcaa tatgatgttt
      121 tctattattt agctatgcaa ccatggagac attttgtatg gactacttgt acaacaactg
      181 atggcaataa agaatgttat aaatatacta tcaatgaaga tcataatgta aaggttgaag
      241 atattaataa aacagatatt aaacaagatt tttgtcaaaa agaatatgca tatccaattg
      301 aaaaatatga agttgattgg gacaatgttc cagttgatga acaacgaatt gaaagtgtag
      361 atattaatgg aaaaacttgt tttaaatatg cagctaaaag accattggct tatgtttatt
      421 taaatacaaa aatgacatat gcaacaaaaa ctgaagcata tgatgtttgt agaatgcatt
      481 tcattggagg aagatcaatt acattcagat catttaacac agagaataaa gcatttattg
      541 atcaatataa tacaaacact acatcaaaat gtcttcttaa agtatatgat aataatgtta
      601 atacacatct tgcaattatc tttggtatta ctgattctac agtcattaaa tcacttcaag
      661 agaatttatc tcttttaagt caactaaaac agtcaagagt aacactctac tatcttaaag
      721 atgtatctta ttttacagtt aatattactt taaatgattt gaaatatgag acacttgtcc
      781 aatacacagc aggttgagga caagttgatc cacttgttaa tcaagctaag aatgacttag
      841 ctactaaagt tgcagataaa agtaaagata aaaatgcaaa tgataaaatc aaaagaggaa
      901 ctatgattgt gttaatggat actgcacttg gatcagaatt taatgcagaa acagaatttg
      961 atagaaagaa tatttcagtt catactgttg ttcttaatag aaataaagac tcaaagaata
     1021 cacgtagtgc attgagactt gtttcacttg gaccacatta tcatgaattc acaggtaatg
     1081 atgaagttaa tgcaacaatc actgcacttt tcaaaggaat tagagccaat ttaacagaaa
     1141 gatgtgatag agataaatgt tcaggttttt gtgatgcaat gaatagatgc acatgtccaa
     1201 tgtgttgtga gaatgattgt ttctatacat cctgtgatgt agaaacagga tcatgtattc
     1261 catggcctaa agctaaacca aaagcaaaga aagaatgtcc agcaacatgt gtaggtttat
     1321 atgaatgtaa agatcttgaa ggatgtgttg ttacaaaata taacgcatct tgtgaaccaa
     1381 aagtgaaatc catggtacca tattgtgata acgataagaa tctaactgaa gtatgtaaac
     1441 aaaaagctaa ttgtgaagca gatcaaaaac caagttctga tggatattgt tggagttata
     1501 catgtgacca aactactggt ttttgtaaga aagataaacg tgctgaaaat atgtgtacag
     1561 gaaagacaaa taactgtcaa gaatatgttt gtgattcaga acaaagatgt actgttcaag
     1621 aaaaggtatg tgtaaaaaca tcaccataca ttgaaatgtc atgttatgta gctaagtgta
     1681 atctcaatac aggtatgtgt gagaacagat tatcatgtga tacatactca tcatgtggtg
     1741 gagattctac aggatcagta tgtaaatgtg attctacaac taataaccaa tgtcaatgta
     1801 ctcaagtaaa aaacggtaat tattgtgatt ctaataaaca tcaaatttgt gattatacag
     1861 gaaaaacacc acaatgtaaa gtgtctaatt gtactgaaga tcttgttaga gatggatgtc
     1921 ttattaagag atgtaatgta acaagtaaaa caacatattg ggagaatgtt gattgttcaa
     1981 acactaagat tgaatttgct caagatggta aatctgaaac tatgtgtaaa ccatattact
     2041 cagctacatg tttgaatgga caatgtgttg ttcaagcagt tggtgatgtt tctaatgtag
     2101 gatctggata ttgttcaatg ggaacagata atattattac atatcatgat gattgtaatt
     2161 cacgtaaatc acaatgtgga aactttaatg gtaagtgtgt agaaaatagt gacaaatcat
     2221 attcttgtgt atttaataag gatgtttctt ctacatcaga taatgatatt tgtgcaaaat
     2281 gttctagttt aacatgtcca gctgatacta catacagaac atatacatat gactcaaaaa
     2341 caggaacatg taaagcaact gttaagccaa caccatcatg ttcagtatgc gaaaaaggta
     2401 aatttgtaga gaaatcgaaa gatcaaaaat tagaacgtaa agtcacttta gaagatggaa
     2461 aagaatatca atacaatatt ccaaaagatt gtgtcaatga acaatgcatt ccaacaagca
     2521 tacatgattg tttaggtaat gatgataact ttaaatctat ttataacttc tatttaccat
     2581 gtcaagcata tgttacagct acctatcatt acagttcatt attcaattta actagttata
     2641 aacttcattt accacaaagt gaagaattta tgaaagaggc agacaaagaa gcatattgta
     2701 catacgaaat aacaacaaga gaatgtaaaa catgttcatt aattgaaact agagaaaaag
     2761 tacaagaagt tgatttgtgt gcagaagaga ctaagaatgg aggagttcca ttcaaatgta
     2821 agaataacaa ttgcattatt gatcctaact ttgattgtca acctattgaa tgtaagattc
     2881 aagagattgt tattacagaa aaagatggaa taaaaacaac aacatgtaaa aatggtacaa
     2941 aaacaacatg tgacactaac aataagagaa tagaagatgc acgtaaagca ttcattgaag
     3001 gaaaagaagg aattgagcaa gtagaatgtg caagtactgt ttgtcaaaat gataatagtt
     3061 gtccaattat tgctgatgta gaaaaatgta atcaaaacac agaagtagat tatggatgta
     3121 aagcaatgac aggacaatgt gatggtacta catatctttg taaatttgta caacttactg
     3181 atgatccatc attagatagt gaacatttta gaactaaatc aggagttgaa cttaacaatg
     3241 catgtttgaa acataaatgt gttgagagta aaggaagtga tggaaaaatc acacataaat
     3301 gggaaattga tacagaacga tcaaatattg atccaaaaca aagaaatcca tgcgaaaccg
     3361 caacatgtga tcaaacaact ggagaaacta tttacccaat gaaaacatgt actgtttcag
     3421 aagaattccc aacaatcaca ccaaatcaag gaagatgttt ctattgtcaa tgttcatatc
     3481 ttgacggttc atcagttctt actatgtatg gagaaacaga taaagaatat tatgatcttg
     3541 atgcatgtgg taattgtcgt gtttggaatc agacagatag aacacaacaa cttaataatc
     3601 acaccgagtg tattctcgca ggagaaatta ataat
//