From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: galK=galactokinase...galM=mutarotase homolog [Lactobacillus
Date: 29 Dec 92 01:21:15 GMT
Distribution: bionet
Checksum: 18471 8

LOCUS       S41325       3316 bp    DNA             NLM       07-DEC-1992
DEFINITION  galK=galactokinase...galM=mutarotase homolog [Lactobacillus
            helveticus, Genomic, 3 genes, 3316 nt]
ACCESSION   S41325 X57248
KEYWORDS    .
SOURCE      Lactobacillus helveticus
  ORGANISM  Lactobacillus helveticus
            Unclassified.
REFERENCE   1  (bases 1 to 3316)
  AUTHORS   Mollet,B. and Pilloud,N.
  TITLE     Galactose utilization in Lactobacillus helveticus: isolation and
            characterization of the galactokinase (galK) and
            galactose-1-phosphate uridyl transferase (galT) genes.
  JOURNAL   J. Bacteriol. 173, 4464-4473 (1991)
  STANDARD  full automatic
COMMENT     This entry [NCBI gibbsq 41325] was created by the journal scanning
            component of NCBI/GenBank at the National Library of Medicine.
            This sequence comes from Figure 2.  The authors begin their
            numbering at 900.
FEATURES             Location/Qualifiers
     CDS             154..1320
                     /note="Description: galactokinase;  For the protein
                     sequence (NCBI gibbsq 41326):  This sequence comes from
                     Figure 2."
                     /gene="galK"
                     /codon_start=1
                     /translation="MNKEELLKKYEATSNEKAKDVFFSPGRINVIGEHTDYNGGHVFP
                     APISLGVYGVYGPRDDKKVRLYSGNVDGDIVEFDIDDTNVEKDEDRFWANYFKGMITY
                     LREKYDGIDHGFNLYIEANLPSGSGLSSSAAIEMLMGIILKDEFNLDVDRVSLAKMGQ
                     RTENEFIGLNSGIMDQFACIMGKKNSAIFLDCNTLKYEYLPLALGDYEIIIMATNNPH
                     TLADSAYNNRVAECGRALKKLQQKLDIKALGELDNDTFDEYSYLINDETEIKRARHAV
                     SENQRTLRATQAMKDGDLEKLGRLINASHESLHYDYEVTGKELDTLAEASWKQPGVLG
                     ARMIGGGFGGSAIAIVKKSEAENFKKNVGKIYRDAVGYDASFYDAEIVDGTKRI"
     CDS             1342..2808
                     /note="Description: galactose-1-phosphate uridyl
                     transferase;  For the protein sequence (NCBI gibbsq
                     41327):  This sequence comes from Figure 2."
                     /gene="galT"
                     /codon_start=1
                     /translation="MKIIEKFADEVINSGAYEPLDRVYVINKIRALVGDHDEEEENDQ
                     PAAKQLVDLAVKNEKIPDDITSREVLNDQLYDLATPTPSKTNSIFWQKMQKSSEENTD
                     WFYKLCEDNNYVKKEAIAKNVVFSGTSSKGHSLEITINLSKPEKDPKAIAAAAHATGK
                     KYPQCALCLENEGYLGGYGKNARSNLRIIRMNIAGRPWGFQYSPYAYFNEHCIFLDQK
                     HIPMVINQQTLINLVEIEKIFPHYFVGSNADLPIVGGSMLAHEHYQGGRHTFPMMKAG
                     IKKNIMFDSYPKVVAGIVDWPMSDLRLTSDNSLDLIDLGSKIIKFWDNYSDTARDIKA
                     YEGETRHHTVTPIMHREGKNFVLDLVLRDNNTSEKYPLGIFHPHKQLWHIKKENIGLI
                     EVMGRAILPGRLKSELEEVKKYWLGEDNKMAASHKEWADQVKAENEISLDNVDQVMEQ
                     SLVEVFEQVLQDAGVFKNNADGEEGWDKFITALTKEIK"
     CDS             2928..3314
                     /note="Description: mutarotase homolog;  For the protein
                     sequence (NCBI gibbsq 41328):  This sequence comes from
                     Figure 2."
                     /gene="galM"
                     /codon_start=1
                     /translation="MKTSFNKYGRKDGKDLCEIVLENDHGMIVKVLNYGATLEKVLLN
                     DENMILSLNSPADYSQERNYLGGTVGRIAGRVRKGQWRHGLETHQLPINDGENHIHGG
                     IGTDTEVWDFKPSCSENSARVDLTLLD"
BASE COUNT     1112 a    509 c    704 g    991 t
ORIGIN      
        1 atgattttct tcaagcgata ttatcagatt ttgtattgca ataaactaaa taatataaat
       61 attttatttg ttaaagtaaa gtatttacta aatagcgtta agagtatata ctaatattga
      121 aaacgattac aggataaatt tgaggtaaaa cttatgaata aagaagaact acttaagaaa
      181 tatgaagcaa cttctaacga aaaggcaaag gatgtatttt tctcaccagg tcgaattaat
      241 gtaattggtg aacatactga ctacaacggt ggacacgtct ttccagcccc aatcagttta
      301 ggtgtttatg gagtctatgg tccaagagac gacaaaaagg tacgccttta ctcaggcaac
      361 gttgacggcg atatcgttga atttgacatt gatgacacaa atgttgaaaa agatgaagac
      421 cgtttctggg ctaactactt taagggcatg atcacttatt tgcgtgaaaa gtatgatggt
      481 attgatcatg gctttaactt gtacatcgaa gctaacttgc catcaggttc aggtctttca
      541 tccagtgcag ccattgaaat gctaatggga atcattttaa aagatgaatt taacttagat
      601 gttgaccgtg tgagtttagc caagatgggc caaagaactg aaaatgaatt tattggcttg
      661 aattcaggaa ttatggatca atttgcctgc attatgggta aaaaaaatag tgctattttc
      721 ctagattgca acaccttaaa gtatgaatac ttaccacttg cattaggtga ttacgaaatc
      781 atcatcatgg ctactaataa tccacatact ttagctgatt ctgcttacaa caatagagtt
      841 gctgaatgtg gtcgtgcact taagaagttg caacaaaagc ttgatattaa ggcattaggt
      901 gagcttgata acgatacttt tgatgaatat tcttacctca ttaatgatga aacagaaatc
      961 aagcgtgctc gtcatgcagt tagcgaaaac caaagaactt tgcgtgcaac tcaagctatg
     1021 aaggatggcg atcttgaaaa gcttggtcgt ttgattaatg cttctcatga atctcttcac
     1081 tacgattatg aagtaactgg taaggaactt gatactttgg cagaggcttc atggaaacaa
     1141 cctggcgtgc tcggtgccag aatgatcggc ggcggtttcg gtggtagtgc cattgcgatc
     1201 gttaagaaat ctgaagctga aaactttaag aagaacgtcg gcaagattta tcgtgatgca
     1261 gttggttatg atgcaagctt ctatgatgca gaaatcgttg acggtactaa gagaatttaa
     1321 ttagtgtgag aaggggaagt tatgaaaatt attgaaaaat ttgcggatga agttataaat
     1381 agtggagcct acgaaccatt agatcgagta tatgtcatta ataaaattag agcattagtt
     1441 ggtgatcatg acgaagaaga agaaaatgat caaccggcag ctaagcagtt agttgattta
     1501 gcagttaaga atgaaaaaat tccggatgac atcacttctc gtgaagtgct aaatgatcaa
     1561 ctttatgatc tagctacccc aacaccatct aagaccaata gtattttctg gcaaaaaatg
     1621 caaaagtctt cagaagagaa cactgattgg ttctataaat tatgtgaaga caacaattat
     1681 gttaaaaaag aagctattgc taagaatgtt gttttctcag gtactagttc taaaggccac
     1741 agcttagaaa ttacaattaa cttatccaag cctgaaaaag atccgaaggc catcgctgcg
     1801 gcagcacatg caacaggtaa gaaatatcct caatgtgcgc tttgtcttga aaatgaaggc
     1861 tatttaggtg gttatggtaa gaatgctcgt tctaatttaa gaattatccg catgaacatt
     1921 gcaggccgtc catggggctt ccaatattca ccttatgcat actttaatga acactgcatt
     1981 ttcttggatc aaaagcatat tccaatggtg attaatcaac agaccttgat taacttggtt
     2041 gaaattgaaa aaatcttccc tcattacttc gttggttcta atgctgattt accaattgtt
     2101 ggtggttcaa tgcttgctca tgaacactat caaggtggtc gtcatacttt cccaatgatg
     2161 aaggctggaa tcaagaagaa tattatgttt gattcttatc ctaaagttgt ggcaggcatc
     2221 gttgattggc caatgagtga tttacgttta acttcagata attcacttga tttgattgat
     2281 cttggcagca agattattaa attctgggat aactattctg atactgcgcg tgatattaag
     2341 gcttatgaag gcgagacacg tcaccatact gtcactccaa tcatgcaccg tgaaggtaag
     2401 aattttgtcc ttgatcttgt tttacgtgat aacaatacta gcgagaaata cccacttgga
     2461 attttccacc cacacaaaca attgtggcac attaagaagg aaaatatcgg cttgattgaa
     2521 gttatgggta gagctatttt gccaggacga cttaagagtg aacttgaaga agtaaagaaa
     2581 tactggcttg gtgaagacaa taagatggct gcaagtcata aagaatgggc tgatcaagtt
     2641 aaggctgaaa acgaaattag cttggataat gttgatcaag tgatggaaca atcacttgtt
     2701 gaagtatttg aacaagttct tcaagatgcc ggtgtattta agaataatgc agatggcgag
     2761 gaaggctggg ataagtttat tacagctttg actaaagaga ttaagtaaag ttattaaatc
     2821 gaggtcttga gagtctcgct ttttgttaac ggtaactcat atgcgattga taaaaaatac
     2881 tttataatag caatgaaaac gaatacaaaa agagaaaagg caaaaatatg aaaacaagtt
     2941 ttaataaata tggcagaaaa gatggtaagg acctttgcga gatcgttttg gaaaatgatc
     3001 atggcatgat tgttaaggtg cttaattatg gtgcaacgct agaaaaagtt ttgcttaatg
     3061 acgagaatat gattttgtca ttgaattcac cagctgatta ttctcaagaa agaaattatt
     3121 taggtggtac agttggccgt attgctggta gagttagaaa aggtcaatgg agacatggac
     3181 ttgaaactca tcaattacca atcaatgacg gtgagaatca tattcacggc ggaattggta
     3241 cagatactga agtctgggat tttaagcctt cttgtagtga aaatagtgca cgagtagatt
     3301 taactttatt ggatcc
//