From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Saccharomyces cerevisiae N-acetyltransferase (MAK) complete cds;
Date: 31 Dec 92 01:49:09 GMT
Distribution: bionet
Checksum: 63335 6

LOCUS       YSCMAKNHP    1780 bp ds-RNA             PLN       18-DEC-1992
DEFINITION  Saccharomyces cerevisiae N-acetyltransferase (MAK) complete cds;
            high mobility group non-histone protein (NHP6A), complete cds.
ACCESSION   M95912
KEYWORDS    N-acetyltransferase; high mobility group non-histone protein.
SOURCE      Saccharomyces cerevisiae cDNA to genomic RNA.
  ORGANISM  Saccharomyces cerevisiae
            Eukaryota; Fungi; Eumycota; Hemiascomycetes; Endomycetales;
            Saccharomycetaceae.
REFERENCE   1  (bases 1 to 1780)
  AUTHORS   Wickner,R.B. and Leibowitz,M.J.
  TITLE     Chromosomal genes essential for replication of a double-stranded
            RNA plasmid of Saccharomyces cerevisiae: the killer character of
            yeast
  JOURNAL   J. Mol. Biol. 105, 427-443 (1976)
  STANDARD  full automatic
REFERENCE   2  (bases 1 to 1780)
  AUTHORS   Tercero,J.C. and Wickner,R.B.
  TITLE     MAK3 encodes an N-acetyltransferase whose modification of the L-A
            gag N-terminus is necessary for virus particle assembly
  JOURNAL   J. Biol. Chem. (1992) In press
  STANDARD  full automatic
REFERENCE   3  (bases 1 to 1780)
  AUTHORS   Tercero,J.C., Riles,L.E. and Wickner,R.B.
  TITLE     Localized mutagenesis and evidence for post-transcriptional
            regulation of MAK3, a putative N-acetyltransferase required for
            double-stranded RNA virus propagation in Saccharomyces cerevisiae
  JOURNAL   J. Biol. Chem. 267, 20270-20276 (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     misc_feature    646..852
                     /evidence="EXPERIMENTAL"
                     /citation=[3]
                     /citation=[2]
                     /note="consensus sequence pattern for
                     N-acetyltransferases"
     CDS             406..936
                     /function="acetylates N-terminus of L-A virus gag protein"
                     /standard_name="Mak3p"
                     /gene="MAK"
                     /evidence="EXPERIMENTAL"
                     /label=MAK3
                     /citation=[3]
                     /citation=[2]
                     /citation=[1]
                     /product="N-acetyltransferase"
                     /codon_start=1
                     /translation="MEIVYKPLDIRNEEQFASIKKLIDADLSEPYSIYVYRYFLNQWP
                     ELTYIAVDNKSGTPNIPIGCIVCKMDPHRNVRLRGYIGMLAVESTYRGHGIAKKLVEI
                     AIDKMQREHCDEIMLETEVENSAALNLYEGMGFIRMKRMFRYYLNEGDAFKLILPLTE
                     KSCTRSTFLMHGRLAT"
     CDS             complement(1139..1420)
                     /standard_name="HMG"
                     /gene="NHP6A"
                     /evidence="EXPERIMENTAL"
                     /citation=[3]
                     /note="this ORF first sequenced by Kolodrubetz and Burgum
                     JBC 265:3234-3239 (1990) who showed that it encodes a
                     high-mobility group non-histone chromatin protein"
                     /product="high mobility group non-histone protein"
                     /codon_start=1
                     /translation="MVTPREPKKRTTRKKKDPNAPKRALSAYMFFANENRDIVRSENP
                     DITFGQVGKKLGEKWKALTPEEKQPYEAKAQADKKRYESEKELYNATLA"
BASE COUNT      594 a    330 c    393 g    463 t
ORIGIN      
        1 aaaatcaaca ttgtagttat acattctcca gatattgcat ttatgatagt ggctgttgtc
       61 tgcagtggtg ttatttcttg ctgggtaggt gcagtgctat attcatcagc gtctgccatg
      121 atgtataaaa gaaaataaaa gaagtacttt ccgaacaaca acagtacact aaaataatcg
      181 ttgttcaaag gaggactact ttggttctga tttgagaaaa tggtactcaa ataagggcat
      241 cgctattata tagttccatt cctaccaaga aaccgggtag ccaaaagcga taaaaaggct
      301 ctccatggca agccttatta aaaataataa tcatgaagaa caaagtttca caagagatta
      361 caagataaaa aagccactac tacagaaaag gcgttgggtc aggacatgga aatagtgtac
      421 aagccattgg acatacgtaa cgaagagcag tttgctagta ttaaaaaact aatagatgcg
      481 gatctatcag agccgtactc gatatacgta tataggtatt tcttgaacca atggcccgag
      541 ttaacgtaca ttgccgttga caacaagagt ggaacgccta acatacccat tggttgcata
      601 gtgtgtaaga tggacccgca tagaaacgtg agactgagag gatatatagg aatgctagcc
      661 gtagagtcca cataccgcgg gcatggcata gcaaagaaac tagtggaaat tgccatagat
      721 aaaatgcaaa gggaacattg cgatgagatc atgttagaaa cagaggtgga aaattcggcg
      781 gctctaaact tatatgaagg aatgggtttc atcagaatga aacggatgtt ccgctactac
      841 ttgaatgaag gggacgcctt taaactgata ttgccgttga ctgaaaagag ctgcactcgg
      901 tctacgttcc taatgcatgg ccggctggcc acataaaagg aaaaacactc gatgatgata
      961 aaatatatat taataataca atttttttat ttatttattt aatcatatgt atagatatag
     1021 aaaaagggca gtagagaaaa gaggaataga aaggagagag ataataaagc aagtttcaaa
     1081 aagaacaaga ggaaaagaaa ttaaatcaca cagacaaaaa cgcgggaagg aagtatccct
     1141 aagccaaagt ggcgttatat aactcctttt cggattcata tctcttctta tcggcctggg
     1201 ccttggcctc gtaaggctgc ttttcctctg gcgttagagc cttccacttc tcacccaact
     1261 tcttgccgac ttgtccaaat gtgatatctg gattttcaga acgaacaata tctctgtttt
     1321 cgttagcgaa aaacatgtag gcggacaaag ccctcttagg ggcatttggg tccttcttct
     1381 ttctagtggt tctcttctta ggttctcttg gggtgaccat tgcgactgtg ctttactatg
     1441 tatagggtag tcaattgagc aaaaacagga aaaaaacacg tcccaaaaca gtccttcttt
     1501 aacagtcggt accagtaata aatactgtgt tgtttgtttg cccaccgcta aataaacgaa
     1561 cggcgaaaaa aataggcggc aaaaggcggg tgattcgaaa agtatcgcgc gcgacgaggc
     1621 gtgagggtag aattcggcgc cactaatttg tgacacttga acagtattta acgtcgtgcg
     1681 atacatcata tcgatagaaa tgtagacgtg ctcaacgctc caagaatgaa ttgcacactt
     1741 acagataata ccagagcata atgtggcctt ccaacctcgg
//