From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Escherichia coli aminodeoxychorismate lyase (pabC) gene, complete
Date: 15 Aug 92 10:01:39 GMT
Distribution: bionet
Checksum: 12334     5

LOCUS       ECOPABC      1667 bp ds-DNA             BCT       15-AUG-1992
DEFINITION  Escherichia coli aminodeoxychorismate lyase (pabC) gene, complete
            cds.
ACCESSION   M93135
KEYWORDS    aminodeoxychorismate lyase; pabC gene.
SOURCE      Escherichia coli (sub_strain W3110, strain K-12) (library: Kohara
            lambda-14C1) DNA.
  ORGANISM  Escherichia coli
            Prokaryotae; Gram-negative facultatively anaerobic rods;
            Enterobacteriaceae.
REFERENCE   1  (bases 1 to 1667)
  AUTHORS   Green,J.M., Merkel,W.K. and Nichols,B.P.
  TITLE     Characterization and sequence of Escherichia coli pabC, the gene
            encoding aminodeoxychorismate lyase, a pyrodoxal phosphate=
            containing enzyme
  JOURNAL   Unpublished (1992)
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     CDS             127..936
                     /function="4-amino-4-deoxychorismate --> 4-aminobenzoate +
                     pyruvate"
                     /note="amino-terminal sequence -
                     NH2-MFLINGHKQESLAVSDRATQF-"
                     /product="4-amino-4-deoxychorismate lyase"
                     /gene="pabC"
                     /evidence=EXPERIMENTAL
                     /codon_start=1
                     /translation="MFLINGHKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMISCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTAYPAHYDRLRNEGITLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEESLQADEMVICNALMPVMPVCACGDVSFSSATLYEYLAPLCERPN"
                     /citation=[1]
     CDS             939..1667
                     /standard_name="ORF"
                     /note="ORF of unknown function"
                     /note="putative"
                     /codon_start=1
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTWMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDKVASVFINRLRIGMRLQ"
                     /citation=[1]
                     /partial
BASE COUNT      403 a    378 c    457 g    429 t
ORIGIN
        1 ggatctaagt tgtcattttc cacccttata aaaggtccgc ttgcggcctt ttttcttagc
       61 ttttattccg acttgttccg tagtgaacat gctgccacac taacaattct ctgataagga
      121 gccggtatgt tcttaattaa cggtcataag caggaatcgc tggcagtaag cgatcgggca
      181 acgcagtttg gtgatggttg ttttaccacc gccagagtta tcgacggtaa agtcagtttg
      241 ttatcggcgc atatccagcg actacaggat gcttgtcagc ggttgatgat ttcctgtgac
      301 ttctggcctc agcttgaaca agagatgaaa acgctggcag cagaacagca aaatggtgtg
      361 ctgaaagtcg tgatcagtcg cggtagtggc gggcgagggt acagcacatt gaacagcgga
      421 ccggcaacgc ggattctctc cgttacggct tatcctgcac attacgaccg tttgcgtaac
      481 gaggggatta cgttggcgct aagcccggtg cggctggggc gcaatcctca tcttgcaggt
      541 attaaacatc tcaatcgtct tgagcaagta ttgattcgct ctcatcttga gcagacaaac
      601 gctgatgagg cgctggtcct tgacagcgaa gggtgggtta cggaatgctg tgcggctaat
      661 ttgttctggc ggaagggcaa cgtagtttat acgccgcgac tggatcaggc aggtgttaac
      721 ggcattatgc gacaattctg tatccgtttg ctggcacaat cctcttatca gcttgtcgaa
      781 gtgcaagcct ctctggaaga gtcgttgcag gcagatgaga tggttatttg taatgcgtta
      841 atgccagtga tgcccgtatg tgcctgtggc gatgtctcct tttcgtcagc aacgttatat
      901 gaatatttag ccccactttg tgagcgcccg aattagtcat gaaaaaagtg ttattgataa
      961 tcttgttatt gctggtggta ctgggtatcg ccgctggtgt gggcgtctgg aaggttcgcc
     1021 atcttgccga cagcaaattg cttatcaaag aagagacgat atttaccctg aagccaggga
     1081 ccggacgtct ggcgctcggt gaacagcttt atgccgataa gatcatcaat cgtccacggg
     1141 tttttcaatg gctgctgcgt atcgaaccgg atctttctca ctttaaagcc gggacttacc
     1201 gctttacacc gcagatgacc gtgcgcgaga tgctgaaatt gctggaaagc ggtaaagaag
     1261 cacagttccc tctgcgactg gtagaaggga tgcgtctgag cgattacctc aagcaattgc
     1321 gtgaggcccc gtatatcaag catacgctga gcgatgataa gtacgccacc gtagcgcagg
     1381 cacttgaact ggaaaacccg gagtggattg aaggttggtt ctggccagac acctggatgt
     1441 ataccgccaa taccaccgat gtcgcgttac tcaagcgagc gcacaagaaa atggtgaaag
     1501 cggtcgatag cgcctgggaa gggcgtgcgg acggtctgcc ttataaagat aaaaaccagt
     1561 tggtgacgat ggcatcaatt atcgaaaaag aaaccgccgt tgccagtgaa cgcgataagg
     1621 ttgcctcagt atttatcaac cgtttacgca ttggtatgcg cctgcag
//