From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: M.bovis adh gene for alcohol dehydrogenase
Date: 28 Jul 92 10:05:55 GMT
Distribution: bionet
Checksum: 02499     4

LOCUS       MSGADH       1619 bp ds-DNA             BCT       28-JUL-1992
DEFINITION  M.bovis adh gene for alcohol dehydrogenase
ACCESSION   X63450
KEYWORDS    ADH gene; alcohol dehydrogenase.
SOURCE      Mycobacterium bovis DNA.
  ORGANISM  Mycobacterium bovis
            Prokaryotae; Firmicutes; Mycobacteria; Mycobacteriaceae.
REFERENCE   1  (bases 1 to 1619)
  AUTHORS   Stelandre,M., Bosseloir,Y., Maes,P., De Bruyn,J. and Content,J.
  TITLE     Alcohol dehydrogenase gene of Mycobacterium bovis BCG: cloning,
            sequence analysis and expression in Escherichia coli
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
COMMENT     *source: strain=ssp.BCG, 1173P2; *source: clone_library=lgt11;
            *source: clone=103;
            From EMBL    entry MBADH;  dated 01-JUL-1992.
FEATURES             Location/Qualifiers
     -35_signal      177..182
     -10_signal      199..204
     RBS             215..220
     CDS             227..1267
                     /EC_number="1.1.1.1"
                     /product="alcoholdehydrogenase"
                     /gene="adh"
                     /codon_start=1
                     /translation="MSTVAAYAAMSATEPLTKTTITRRDPGPHDVAIDIKFAGICHSD
                     IHTVKAEWGQPNYPVVPGHEIAGVVTAVGSEVTKYRQGDRVGVGCFVDSCRECNSCTR
                     GIEQYCKPGANFTYNSIGKDGQPTQGGYSEAIVVDENYVLRIPDVLPLDVAAPLLCAG
                     ITLYSPLRHWNAGANTRVAIIGLGGLGHMGVKLGAAMGADVTVLSQSLKKMEDGLRLG
                     AKSYYATADPDTFRKLRGGFDLILNTVSANLDLGQYLNLLDVDGTLVELGIPEHPMAV
                     PAFALALMRRSLAGSNIGGIAETQEMLNFCAEHGVTPEIELIEPDYINDAYERVLASD
                     VRYRFVIDISAL"
BASE COUNT      309 a    530 c    518 g    262 t
ORIGIN
        1 cgtcatcctg gatagcgacc catggcgcaa gctgtccgcc aaccgtgaca accgggtctt
       61 cgtcgtcaac gaccaggtat ggcagaccgg cgagggtatg gtcgctgccc gcggcattgt
      121 cgatgatctg cgctgggtcg acgtgccgat caactagtga ggcgcagcgc taggctttgg
      181 gatacccaca gctaaaaaat taatcaaaga aacgaagagg gttgccatga gcactgttgc
      241 cgcctacgcc gccatgtcgg cgaccgaacc cctgaccaag accacgatca cccgtcgcga
      301 cccgggcccg cacgacgtgg cgatcgacat caagttcgcc ggaatctgtc actcggacat
      361 ccataccgtc aaagccgagt ggggccaacc gaattaccct gtggtccctg gccacgagat
      421 cgccggcgtg gtgaccgccg tgggctcgga ggtgaccaag taccggcagg gcgaccgcgt
      481 tggggttggc tgtttcgtgg actcgtgccg cgagtgcaac agttgcacgc gcggcatcga
      541 acagtactgc aagccgggcg caaacttcac ctacaactcg atcggcaaag acggccagcc
      601 aacccagggc ggctacagcg aagcgatcgt cgtcgacgaa aactacgtgt tgcgcatacc
      661 cgacgtgctg cccctggatg tggcggcgcc gctgttgtgc gcgggcatca cgctgtactc
      721 gccactgcgc cactggaatg ccggggcgaa cacgcgggtg gcgatcatcg gcctaggcgg
      781 actgggtcac atgggcgtca agctgggcgc cgcgatgggc gccgacgtga cggtgctgtc
      841 ccaatcgctg aagaaaatgg aggacggtct gcgcttgggg gccaagagct actacgcgac
      901 cgccgacccg gacaccttcc gcaagctgcg cggcggcttc gacctgatcc tgaacaccgt
      961 ctcggctaac ttggacctcg gccagtacct gaacctgctg gacgtcgacg gcacactcgt
     1021 ggaactgggt atccccgagc accccatggc cgtgccggcg ttcgcgctag cgctcatgcg
     1081 acgcagcctg gccgggtcca acatcggcgg gatcgccgag acccaggaga tgctcaattt
     1141 ctgtgccgag cacggcgtga cacccgaaat cgagctgatt gaaccggact acatcaacga
     1201 cgcctacgag cgcgtgctgg ccagcgacgt gcgctaccgc ttcgtcatcg acatctcagc
     1261 cctgtgaggc cggtgcgcga tcacttccgg attcggactc gccgacgtcg acgccggcca
     1321 gcggccatcc ggcggcggcc aggatgcctg ccacccgttg gatgttttcc ggtccggcgt
     1381 cgtgatgggt cacctcggag atgaactcgg cgatctcgtc gcggtcgatc acacggtccg
     1441 ccacggcggg cgacccgttc tcggtgaagt ggcgcaccac ctccccgatc tgctcctcgg
     1501 tcagcggggt gctgcgcaat agcgacagca gcgccacccg gtccggcccg gggacgccct
     1561 cggggtagcc aacctgaagc cagcgcagca ccgaacggaa gaaatgcggg tgcgagaac
//