From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: S.ambofaciens genes for SrmB, SrmR and SrmX
Date: 21 Jul 92 10:08:38 GMT
Distribution: bionet
Checksum: 55984    11

LOCUS       STMSRMBRX    5704 bp ds-DNA             BCT       21-JUL-1992
DEFINITION  S.ambofaciens genes for SrmB, SrmR and SrmX
ACCESSION   X63451
KEYWORDS    SrmR gene; SrmX gene; srmB gene.
SOURCE      Streptomyces ambofaciens DNA.
  ORGANISM  Streptomyces ambofaciens
            Prokaryotae; Firmicutes; Streptomycetaceae.
REFERENCE   1  (bases 1 to 5704)
  AUTHORS   Losick,R.
  JOURNAL   Unpublished (1991)
  STANDARD  full automatic
REFERENCE   2  (sites)
  AUTHORS   Geistlich,M., Turner,J.R., Losick,R. and Rao,N.R.
  TITLE     Characterization of a novel regulatory gene governing the
            expression of a polyketide synthase gene in Streptomyces
            ambofaciens
  JOURNAL   Unpublished (1992)
  STANDARD  full staff_review
COMMENT     *source: strain=BES2281;
            From EMBL    entry SASRMBRX;  dated 09-JUN-1992.
FEATURES             Location/Qualifiers
     CDS             558..2210
                     /product="SrmB"
                     /codon_start=1
                     /translation="MSIAQYALHDITKRYHDCVVLDRVGFSIKPGEKVGVIGDNGSGK
                     STLLKILAGRVEPDNGALTVVAPGGVGYLAQTLELPLDATVQDAVDLALSDLRELEAA
                     MREAEAELGESDENGSERELSAGLQRYAALVEQYQARGGYEADVRVEVALHGLGLPSL
                     DRDRKLGTLSGGERSRLALAATLASSPELLLLDEPTNDLDDRAMEWLEDHLAGHRGTV
                     IAVTHDRVFLDRLTTTILEVDSGSVTRYGNGYEGYLTAKAVERERRLREYEEWRAELD
                     RNRGLITSNVARMDGIPRKMSLSVFGHGAYRRRGRDHGAMVRIRNAKQRVAQLTENPV
                     HAPADPLSFAARIDTAGPEAEEAVAELTDVRVAGRLAVDSLTIRPGERLLVTGPNGAG
                     KSTLLRVLSGELEPDGGSVRVGCRVGHLRQDETPWAPGLTVLRAFAQGREGYLEDHAE
                     KLLSLGLFSPSDLRRRVKDLSYGQRRRIEIARLVSDPMDLLLLDEPTNHLTPVLVEEL
                     EQALADYRGAVVVVTHDRRMRSRFTGARLTMGDGRIAEFSAG"
     CDS             complement(2321..4135)
                     /product="SrmR"
                     /codon_start=1
                     /translation="MRRLERFNRLALTAQSMIEYRRDREAELAALVDAAHEFVAARRG
                     KDLLESIARRARLLLKLDVSYVGLHEEDRPGTVVLSADGNAVKVAESYRLPADGGLGA
                     MVRTCRAPFWTPDYLGDNSFTHVEAVDDIVRAEGLRAVLAVPLCAGGEPMGVLYVADR
                     QVRHLTPNEVTLLCSLADLAAVAIERNRLVEELHDTIGQLRQDIGEARTALARTRRSA
                     DLQSHLVTQVMDRRGADSLLATAAEALGGGAGLCSPLGRPLAEYGTLRPVAPTELRAA
                     CRRAAETGRPTSVAPGVWTVPLLPGGNAGFLLTDLGPDADHTAVPLLPMVARTLALHL
                     RVQHDDSPKAQSHQEFFDDLIGAPRSPTLLRERALMFSLSFRRPHVVLVAGGPRGTSP
                     RLDRSGADYAKELGGLCSVRDGAVVLLLPGDDPVAVAQTAAPELTDRAGHPVTVGVAG
                     PASTVDGIADAHREAAKCLETLRALGGDGGTACASDLGFLGMLLAEENDVPGYIRTTI
                     GPVVDYDTHRFTDLVPTLRVYLESGRSPTRAAETLRVHPNTVSRRLERIGVLLGEDWQ
                     SPERVLDIQLALRLYQVRSALSSQPASETRAVLGSLRE"
     CDS             4863..5582
                     /product="SrmX"
                     /codon_start=1
                     /translation="MYENDSAAEVYDLLYQDRKDYAGEAARVTDLIRERTPDAASLLD
                     IACGTGTHLEAFAKLYDRVSGLELSEWMAARAEERLPGVTLHRGDMRAFDLGETFDAV
                     VCMFSSIGYLETTADLEDAVAAMARHLTADGVLAVEPWYFPDTFLDGHVSTHALRTAP
                     GDQGVARVSHSTREGGRTRMEIHYLIAHTAEGIRHRSEVDYLTLFSRAEYEAAYRKAG
                     LDVEYVVTGEGSPGFFLGTRR"
BASE COUNT      824 a   2023 c   2039 g    818 t
ORIGIN
        1 ggtaccgggc gaactgccgc tcgaagcccc gggaggccgg gccggccgcg atccgcatct
       61 ccagcgccgt ctccaccagc gccagccggt cctcctcgct cagcaccgcg ccggagaccg
      121 gtacgtgcgt cgtcccggga acgaagcccc cgggcgcctg ttcccggtgg tacgcgcgga
      181 ccagctcggt gatgcggttc ctgagatcgc ccatggtcga cccctctcgt gaaagaccgc
      241 tgtggttgct cccgaggcta ggcatccggc cgccggacaa gcgatcatcc cggtccgact
      301 gcatcacgtc ggccggtgcg acacgcatac cgttgcgcgg caccgggtag cacggctata
      361 gttcttcgca tgggatctct gtgatagatg aggatgcccg gcgttgagct gactccgcct
      421 gccgcatcca gcagcgtgct cgtgacaccc cctgaccggc catgtccgca gtggtggcgt
      481 cccttctgct ttccgttctt tctgcgagcg cgctcacgca tccactcatc ctcatcgtgg
      541 aaacaggaga cttacgcgtg tcgattgcgc aatacgccct acacgacatc acgaagcgct
      601 accacgactg tgtcgtgctc gaccgggtcg gtttcagcat caagccgggc gagaaggtcg
      661 gcgtgatcgg cgacaacggt tccggcaagt ccacgctgct caagatcctc gccggccgcg
      721 tggagcccga caacggcgcg ctcaccgtgg tcgctcccgg cggcgtcggc tacctggcgc
      781 agacactgga actgcccctc gacgccaccg tccaggacgc cgtcgacctg gccctgtccg
      841 acctgcgcga gctcgaagcg gcgatgcgcg aggccgaggc ggagctgggc gagagcgacg
      901 agaacggctc cgagcgcgag ctgtccgccg gcctccagcg ctacgccgct ctggtcgagc
      961 agtaccaggc gcgtggcggc tacgaggccg acgtgcgcgt ggaggtcgcg ctgcacggcc
     1021 tcggactgcc gagcctggac cgcgaccgca agctcggaac cctctccggt ggcgaacgct
     1081 cccgcctcgc gctcgccgcg accctcgcct cgtcgccgga gctgctgctc ctggacgaac
     1141 cgaccaacga cctcgacgac cgggcgatgg aatggctgga ggaccacctg gccggccacc
     1201 gcggcacggt gatcgcggtc acccacgacc gggtcttcct cgaccggctc accaccacga
     1261 tcctggaggt cgactccggc agcgtcaccc gctacggcaa cggctacgag ggctacctga
     1321 cggccaaggc cgtggaacgc gagcggcggc tgcgggagta cgaggagtgg cgtgccgaac
     1381 tcgaccgcaa ccgcgggctg atcacctcca acgtggcgcg gatggacggc atcccgcgca
     1441 agatgtccct ctccgtgttc ggccacggcg cctaccgcag gcgagggcgc gaccacggcg
     1501 cgatggtgcg gatccgcaac gcgaagcaac gcgtggcgca gctgaccgag aacccggtcc
     1561 acgctcccgc cgacccgttg tccttcgccg cccgcatcga caccgcgggc ccggaggcgg
     1621 aggaggcggt ggccgaactc accgacgtgc gcgtcgcggg tcggctcgcc gtggactccc
     1681 tgacgatccg gcccggcgaa cggctgctcg tcacaggtcc caacggtgcg ggcaagtcca
     1741 ccttgttgcg ggtgctgtcc ggggaactgg agccggacgg cggctcggtg cgcgtcggct
     1801 gccgggtcgg tcatctgcgg caggacgaga cgccctgggc gcccggactg accgtgctgc
     1861 gggccttcgc ccagggccgg gagggctacc tggaggacca cgcggagaaa ctgctgtcgc
     1921 tcggcctgtt cagcccgtcc gacctgcggc gacgcgtgaa ggatctgtcc tacgggcagc
     1981 gccgccggat cgagatcgcc cggctggtga gcgacccgat ggacctgctg ctgctggacg
     2041 agcccaccaa ccacctcacc ccggtgctgg tggaggagtt ggagcaggca ctcgcggact
     2101 accgcggcgc cgtcgtggtc gtcacccacg accgtcggat gcggtcccgg ttcaccggcg
     2161 cccggctgac catgggagac gggcgcatcg ccgagttcag cgccggctga accacgcggg
     2221 cccgggacct cgtcgtacgg gggaccggtg gccggtggtc aacgcccgcc tgtcggcgcg
     2281 atccgcgccg acgggcgggg cgccgcccgt gccggagagg tcactcgcgc agcgatccga
     2341 gcacggcccg ggtctcggac gccggttgcg aggagagcgc cgagcgcacc tgatagagcc
     2401 gcagggccag ttgtatgtcc agcacccgct ccggtgactg ccagtcctct cccagcagta
     2461 cgccgatgcg ctccagccgc cgtgagacgg tgttcgggtg cacgcgcagt gtctctgcgg
     2521 cacgcgtggg gctcctgccc gactccaggt acaccctcag agtgggaacc agatccgtga
     2581 agcggtgggt gtcgtagtcg accacggggc cgatcgtcgt cctgatgtaa ccggggacgt
     2641 cgttctcctc ggcgaggagc atgccgagga aacccaggtc ggaggcgcac gcggtgccgc
     2701 cgtcgccgcc gagcgcgcgg agggtctcca gacacttcgc ggcctcacgg tgcgcgtcgg
     2761 cgatgccgtc gacggtcgag gcggggcccg cgacccccac ggtgacgggg tgcccggcgc
     2821 ggtcggtcag ctccggggcg gcggtctgcg ccacggcgac ggggtcgtcg ccgggcagca
     2881 gcaggacgac ggcgccgtcc cgcacgctgc acagcccgcc gagctccttc gcgtagtcgg
     2941 cgccggaccg gtccagccgc ggcgaggtcc cgcggggtcc gcccgccacc agcaccacgt
     3001 gcgggcggcg gaagctgagg gagaacatca gggcgcgttc cctgaggagc gtgggtgagc
     3061 ggggcgcccc gatcaggtcg tcgaagaact cctggtggct ctgcgccttg ggggagtcgt
     3121 cgtgctggac gcgcaggtgc agcgcgaggg tgcgggcgac catcgggagc agggggacgg
     3181 cggtgtggtc cgcgtccgga ccgaggtcgg tcagcaggaa gccggcgttg cccccgggaa
     3241 gcaggggcac cgtccagacc cccggggcca cggaggtggg ccggccggtc tcggcggccc
     3301 ggcggcacgc cgcgcgcagt tccgtggggg cgacggggcg cagggtcccg tactcggcga
     3361 gcgggcgccc gagcgggctg cacaggccgg ctccgccgcc gagcgcctcg gcggccgtcg
     3421 cgagtaacga gtcggcgccg cgcctgtcca tcacctgcgt gaccaggtgc gactggaggt
     3481 cggcggacct gcgggtgcgc gcgagggcgg tgcgggcctc gccgatgtcc tggcgcagtt
     3541 gcccgatggt gtcgtggagc tcctcgacca gccggttgcg ctcgatcgcc accgcggcca
     3601 gatcggcgag cgagcacagc agggtgacct cgttgggggt cagatgccgc acctgacggt
     3661 cggcgacgta gaggaccccc atcggttcgc ccccggcgca cagcgggacg gccaggaccg
     3721 cgcgcaggcc ttcggcgcgg acgatgtcgt cgacggcctc gacgtgcgtg aagctgttgt
     3781 ccccgaggta gtccggggtc cagaagggag cgcggcaggt gcgcaccatg gcgcccagtc
     3841 cgccgtcggc cggcagccgg tagctctcgg cgaccttgac cgcgttgccg tcggcgctca
     3901 gcaccaccgt gccgggccgg tcctcctcgt gcaggccgac gtaggagacg tccagcttca
     3961 gcagcagccg tgctctgcgg gcgatggact ccagcaggtc cttgccccgc cgggcggcga
     4021 cgaactcgtg cgcggcgtcg accagggccg cgagctccgc ctcccggtcg cggcggtact
     4081 cgatcatcga ctgcgcggtg agggcgagcc ggttgaagcg ctccagtcgg cgcatccggt
     4141 cctcgtcggc gcccgagcgt tcggcgcggg ccatgagccg gtcgtattcg ctccgtggtg
     4201 ccgaccgggc gatgaactcg aggaaggcga gtgcgtcgtc cgactcgtct tcttcggacc
     4261 cttcttcacc agaacccagg tcactcatcc cgcaggcacc cccatccctg tgctttacca
     4321 aagcattgac aggctattac gctttcaagg attccgtcta gatcgaccga ggtgaccgtt
     4381 cgtccccttc gtccgtcttc ggcggcttca gcggaggaca tactctattc gacaagagag
     4441 ggcgcctggc tgaaaggccg gggcttcatg gccgatatcc ggccgtcctt cagtgggtgt
     4501 tgcggcacgg tggaaatgaa ttccataaag gccgggccgg ggttgaaggc tgcctcttca
     4561 accgctgcgg tgaacgaggc ataagctacc cggtggcccg ggggccacac cgtgatttcc
     4621 ggcggaacga gcaaccggtt ggccgaatcc gtgcgcgtcg ccggcgcgcc ccgccccggg
     4681 ggcggagcgc ccgacttgac tgaccacccc gtcgttcctt gaccccggct cggcgtgaac
     4741 tgttgggtgt ccgtacggag atcatcgcga ggggccggcg gcaggccgcc cccgccccga
     4801 gcgcacggcg cactcctctt cgacagaccc acggttcccc cttcacggcc cggaggattc
     4861 ccatgtacga gaacgacagt gccgccgagg tctacgacct gctctaccag gaccgcaagg
     4921 actacgcggg tgaggccgcc cgggtcaccg acctgatccg ggaacgtacg ccggacgcgg
     4981 ccagcctgct cgacatcgcg tgcggcaccg gtacccacct ggaggccttc gccaagctct
     5041 acgaccgcgt gagcggcctg gagctgtccg agtggatggc ggcccgcgcc gaggagcggc
     5101 tccccggcgt caccctccac cgcggtgaca tgcgggcgtt cgacctcggc gagacgttcg
     5161 acgccgtggt ctgcatgttc agctcgatcg ggtacctgga gaccacggcc gacctggagg
     5221 acgccgtcgc cgccatggcg cggcacctga ccgcggacgg tgtcctggcc gtcgaaccgt
     5281 ggtacttccc cgacaccttc ctggacggcc acgtctccac ccacgccctg cggacggcac
     5341 cgggcgacca gggcgtcgcc cgtgtctccc actcgacccg ggagggcggg cggacccgga
     5401 tggagatcca ctacctgatc gcgcacaccg cggagggcat ccggcaccgc agcgaggtgg
     5461 actacctcac gctgttctcg cgtgcggagt acgaggccgc gtaccgcaag gccggcctgg
     5521 acgtcgagta cgtcgtgacg ggcgagggct caccgggctt cttcctcggc acgcgtcgct
     5581 gacgcaccga cgggacggcg gggtggcccg gaccgtccgc aggacgcgcc acggcccctg
     5641 tcccggcggt caccgccggg acaggggccg tggcgcgtgc gcgcgtcagg cgggggccgg
     5701 atcc
//