From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: H.sapiens M gene for M1-type and M2-type pyruvate kinase
Date: 22 Jul 92 10:03:06 GMT
Distribution: bionet
Checksum: 02979    18

LOCUS       HUMPKM12    10368 bp ds-DNA             PRI       22-JUL-1992
DEFINITION  H.sapiens M gene for M1-type and M2-type pyruvate kinase
ACCESSION   X56494
KEYWORDS    pyruvate kinase.
SOURCE      Homo sapiens DNA.
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 10368)
  AUTHORS   Takenaka,M.
  JOURNAL   Unpublished (1990)
  STANDARD  full automatic
REFERENCE   2  (bases 560 to 1134)
  AUTHORS   Takenaka,M., Noguchi,T., Sadahiro,S., Hirai,H., Yamada,K.,
            Matsuda,T., Imai,E. and Tanaka,T.
  TITLE     Isolation and characterization of the human pyruvate kinase M gene
  JOURNAL   Eur. J. Biochem. 198, 101-106 (1991)
  STANDARD  full automatic
COMMENT     From EMBL    entry HSPKM12;  dated 12-MAY-1992.
FEATURES             Location/Qualifiers
     mRNA            join(1005..1127,1309..1475,1687..1778,1870..2001,
                     2129..2315,2409..2679,2764..2914,3162..3314,7395..7561,
                     9359..9540,9540..>10337)
                     /note="alternate"
                     /evidence=EXPERIMENTAL
                     /partial
     mRNA            join(1029..1127,1309..1475,1687..1778,1870..2001,
                     2129..2315,2409..2679,2764..2914,3162..3314,7395..7561,
                     9359..9540,9540..>10337)
                     /note="alternate"
                     /evidence=EXPERIMENTAL
                     /partial
     CDS             join(1322..1475,1687..1778,1870..2001,2129..2315,
                     2409..2679,2764..2914,3162..3314,7395..7561,9359..9540,
                     9626..9732)
                     /EC_number="2.7.1.40"
                     /product="pyruvate kinase"
                     /codon_start=1
                     /translation="MSKPHSEAGTAFIQTQQLHAAMADTFLEHMCRLDIDSPPITARN
                     TGIICTIGPASRSVETLKEMIKSGMNVARLNFSHGTHEYHAETIKNVRTATESFASDP
                     ILYRPVAVALDTKGPEIRTGLIKGSGTAEVELKKGATLKITLDNAYMEKCDENILWLD
                     YKNICKVVEVGSKIYVDDGLISLQVKQKGADFLVTEVENGGSLGSKKGVNLPGAAVDL
                     PAVSEKDIQDLKFGVEQDVDMVFASFIRKASDVHEVRKVLGEKGKNIKIISKIENHEG
                     VRRFDEILEASDGIMVARGDLGIEIPAEKVFLAQKMMIGRCNRAGKPVICATQMLESM
                     IKKPPPTRAEGSDVANAVLDGADCIMLSGETAKGDYPLEAVRMQHLIAREAEAAIYHL
                     QLFEELRRLAPITSDPTEATAVGAVEASFKCCSGAIIVLTKSGRSAHQVARYRPRAPI
                     IAVTRNPQTARQAHLYRGIFPVLCKDPVQEAWAEDVDLRVNFAMNVGKARGFFKKGDV
                     VIVLTGWRPGSGFTNTMRVVPVP"
     misc_signal     593..598
                     /note="GC box"
     misc_signal     765..770
                     /note="GC box"
     protein_bind    942..952
                     /bound_moiety="SP1"
     misc_signal     983..988
                     /note="GC box"
     misc_signal     988..993
                     /note="GC box"
     exon            1005..1127
                     /number=1
     prim_transcript 1005..>10337
     intron          1128..1308
                     /number=1
     exon            1309..1475
                     /number=2
     intron          1476..1686
                     /number=2
     exon            1687..1778
                     /number=3
     intron          1779..1869
                     /number=3
     exon            1870..2001
                     /number=4
     intron          1871..2128
                     /number=4
     exon            2129..2315
                     /number=5
     intron          2316..2408
                     /number=5
     exon            2409..2679
                     /number=6
     intron          2680..2763
                     /number=6
     exon            2764..2914
                     /number=7
     intron          2915..3161
                     /number=7
     exon            3162..3314
                     /number=8
     intron          3315..7394
                     /number=8
     exon            7395..7561
                     /number=9
     intron          7562..9358
                     /number=9
     exon            9359..9540
                     /number=10
     intron          9541..9624
                     /number=10
     exon            9625..>10337
                     /number=11
     polyA_signal    10332..10337
BASE COUNT     2254 a   2784 c   2682 g   2648 t
ORIGIN
        1 ggtcttcaca ttttgaatgc gcaacattgt atctgtgaat gaaggcaaga gttaacagct
       61 gtttaattga taactgctcg catcattagt tgctggctaa caactgggaa atcagaaaat
      121 gtcttgtaga aaaatgtaag aaaagttcca acaatactga cttaaacacg agcaaaggtg
      181 aaaacagaaa tgctgactcc tgcataggtt atcggcccta atgttctgac ttgatatttc
      241 cagatgccca gctctgcgct aatatcaaca ccgtctattt actttctact ctgaggcatt
      301 cgctctgcag gattccagac cctactaaat tattcacatg gccccaaccg gtccttcctt
      361 gttccgcggt cctaacacaa tgaatggtcc taagaggaaa acggcctcgg ctcccgctcc
      421 aggcccactt cgcagtccct agttctccct actgccgctc cagtgccaga gcccctccga
      481 aggcggccag gacctccaac cacgcacaag tctgcagctc tccccaactt tccgttcagc
      541 tcagtctccg agggtgcgcc agagcagaca cccggaggag tggggagtgg cagggcgggg
      601 ccgggagaat gctgccccgg aacccataaa ttcggccctg cccaggtagg ccgggacagc
      661 tggggtggcc tgggccgaga gccaagaaaa gagaccccat ctggacgccc aacttggcgg
      721 caacaggtgg ccggcgcccg ggggtctggg aggaaagtcg ctccgggcgg gccccgttgc
      781 cccgccgcgt ccccattggt catcaggttt cttaaaatgt gactctgaat ctgtgtcctt
      841 ccgccgcaga atttagtccc accgaaaggg caacctgccc gcgcgttccg ccaccgccgc
      901 cgcgcttcct cctgaaggtg actcgagccc gcggggacgc agggggcggg gcccgggtcg
      961 cccggagccg ggattgggca gagggcgggg cggcggaggg attgcggcgg cccgcagcgg
     1021 gataaccttg aggctgaggc agtggctcct tgcacagcag ctgcacgcgc cgtggctccg
     1081 gatctcttcg tctttgcagc gtagcccgag tcggtcagca gccggaggtg agcggtgcag
     1141 gcagtacgcc atcagtcccc accaagggcc agtcgcccgg ctagtgcgga atcccggcgc
     1201 gccggccggc cccgggcacg caggcagggc ggcgcaggat ccctgtgcta aatggtatat
     1261 taaccacttc tcagtcttac cactctcttt caatttgtct cgacccagga cctcagcagc
     1321 catgtcgaag ccccatagtg aagccgggac tgccttcatt cagacccagc agctgcacgc
     1381 agccatggct gacacattcc tggagcacat gtgccgcctg gacattgatt caccacccat
     1441 cacagcccgg aacactggca tcatctgtac cattggtgag tgggtgtccc ccttccccca
     1501 aaaagggctt catgggcagt gacctttctc tcctgaaaag agctccatgc actttttaaa
     1561 gacttttgag ctatttggga gaggaaaaat tttcagggaa aaaaattctt taaacttaaa
     1621 gcaaacttaa atgtttttcc ttggttgaat aattaatact tgtggcttta aaacttttcc
     1681 taataggccc agcttcccga tcagtggaga cgttgaagga gatgattaag tctggaatga
     1741 atgtggctcg tctgaacttc tctcatggaa ctcatgaggt gagctgtggc tggaccctat
     1801 cctggcaggg gaattggagc tggattctag tgtgggagca cgcttgtcat cttccttctt
     1861 ttcccccagt accatgcgga gaccatcaag aatgtgcgca cagccacgga aagctttgct
     1921 tctgacccca tcctctaccg gcccgttgct gtggctctag acactaaagg acctgagatc
     1981 cgaactgggc tcatcaaggg cgtgagtatt ctgcggagag cgaggggaag gctcagtagg
     2041 caatatgccc cagagacatg attccttccg aggtgatgct gctactggtg tctccagttt
     2101 ggactcttcc ttactctctt gtccctagag cggcactgca gaggtggagc tgaagaaggg
     2161 agccactctc aaaatcacgc tggataacgc ctacatggaa aagtgtgacg agaacatcct
     2221 gtggctggac tacaagaaca tctgcaaggt ggtggaagtg ggcagcaaga tctacgtgga
     2281 tgatgggctt atttctctcc aggtgaagca gaaaggtacg tatgggagct ggagtccagt
     2341 tgtctaaaac agtcttttgt ctctaaactt ctcgtctctg cctccccaac ttaccctttt
     2401 ttatacaggt gccgacttcc tggtgacgga ggtggaaaat ggtggctcct tgggcagcaa
     2461 gaagggtgtg aaccttcctg gggctgctgt ggacttgcct gctgtgtcgg agaaggacat
     2521 ccaggatctg aagtttgggg tcgagcagga tgttgatatg gtgtttgcgt cattcatccg
     2581 caaggcatct gatgtccatg aagttaggaa ggtcctggga gagaagggaa agaacatcaa
     2641 gattatcagc aaaatcgaga atcatgaggg ggttcggagg caagtccccg ttgtccctgg
     2701 tctactgcca tacttgtggc ctctgttcta tataacctct ctccccccca ctttgtccat
     2761 caggtttgat gaaatcctgg aggccagtga tgggatcatg gtggctcgtg gtgatctagg
     2821 cattgagatt cctgcagaga aggtcttcct tgctcagaag atgatgattg gacggtgcaa
     2881 ccgagctggg aagcctgtca tctgtgctac tcaggcatgt gcccaccctt ccccacattc
     2941 tcatgtgcac actcgcatgt ttgtatggga aagctctgga ggctgtctga tctcttccca
     3001 tggaattgtc gcaacgtaac acacagataa tccccttccc ccatgtacct acacaaagcc
     3061 atactctgtg tacctactca ctatccagag gatcagcttg ctgtcatttg tctctgaaga
     3121 cagctcaagc tacatctcac taatgctctg tcccctccca gatgctggag agcatgatca
     3181 agaagccccc gcccactcgg gctgaaggca gtgatgtggc caatgcagtc ctggatggag
     3241 ccgactgcat catgctgtct ggagaaacag ccaaagggga ctatcctctg gaggctgtgc
     3301 gcatgcagca cctggtgagt tctgggcctg ccccatcccc cagggcttcg gactgggcct
     3361 gggatggatg caagctctgg tgcagagctt tttaggtttc tccatcctct tatgcacagc
     3421 ctttcattat cctccaagtt acagcagcaa gagggtgggg gtggaagtgg aggtggcttt
     3481 ttttttttct cctgttcctg cattcctgcc cacaccccca cccctctcat ttccttctgc
     3541 tctggaggca cctccttcat tggacaccac acagtttatt tcacttctga cttcaaggtt
     3601 gtgaattctt cccatggctt aagtcctggg atacttctgc agtgaaagga ggtcttgtac
     3661 ctcttcctca gagtcagaag ttctgagtac ctttgcccta ttctgaaaag ggctaggggc
     3721 tcctgctccc agctgccctc ttcctttggc ttccaattca gttccctctg ccccgcatcc
     3781 tgcagacagg cgctcccgca gggggccctt gtggacctgc actggagtct gttgccttca
     3841 ctgagctgcc tgtgctggcc ttgcatggtg cctgtagggg gatttgcttt gctgtgccat
     3901 tggggtacag ctgctgctct tactctagac caaaaagtcg ggttgagtga ctggtggcag
     3961 ggccaagata gagacagcgg ggagggtggc tgaccctggc ggccctggac tgagcgtctg
     4021 gaggagtcgt ggaggctctt tcccttcttt ctcctctgag agctcgttct tcaggctctt
     4081 ccagcttgtc atgtcgagtg cctggccact gctcagggtt ggaggctcag tccctttgcc
     4141 ctgtctgttc cagctctgga gctaactcag ggatccctga tcagggttac gtaggtttgg
     4201 taaaatgagt gctggaaatt aactttctcc cagtagtctt aggtctagct cagtgaactt
     4261 aaactttatc cagatatggt ttttccttca gcctttctat tccctttcta gccagtgaaa
     4321 gacccgctgc cctttgacct cagccccctc caagccccca agtttaaaac gccaccccct
     4381 gccaccagaa aaaacagaaa aaaaaaaaaa aaaaaaaact aaaacaccca tctggtctgg
     4441 gcatcttcct tcctttttca ctatgtatcc tgttactggg cttaaacagc tttcagagaa
     4501 gagatgtcat ttctattaaa tgctctttca gtagcgaact gagttcacac ttgactaagg
     4561 atattttccg gactgtctgt catcagcatc cttagtgggt ttccccatat ttaaattggt
     4621 agaggccagg gatggtggct cacacctgta atctcagtac tttgggaggc caaggtaggt
     4681 ggattgcttg agctcagaag accagcctgg gcaacctggt gaaaccctgt ctctactaaa
     4741 aattcaagtt agctagctgg gcatggtgat gcacttctgt agtcccagct acttggagag
     4801 ggggtggtgc tggggcagca ggatcgctta aacccaggag gttaaggttg cagtcagcca
     4861 agatggtacc agcctaggtg acaaagtgac accctgtctc aaaaaagaaa ccaaacaaac
     4921 ataaaaaaaa aaacaaaaaa atcggtagag agtgatttct ctcccaggcc cacttaatgt
     4981 agactgggcc tggctgacac ctcaccattc gtgtgatgtg attgctgttc tgatgcttag
     5041 atactcttgg cgcagtctca caattgccac catggtagga aggtgtccag gagacggtgc
     5101 accttgaacc agtcaccact aaagtggctg cctttctggg tctctccaca catcccctct
     5161 ctctaatttc cctacttaat cgtgtgactt catggtctca aaggaggaac agaggctgat
     5221 cttgacttag atatactgaa ccatgaaatc actgcataga atgtggggac ttgaatgtgt
     5281 ctttgggcaa gtcatttaac ctcttaagac ctcatctgta aaatggatta gatatgttta
     5341 attatagcct tagcattaaa tattcattgc tgttattatt aagtgtctga taagtctctg
     5401 tgtacatgga tgtaatcttc ctaactccca ttacctccat ttatagatga gggttatatg
     5461 gccaataaag cctgggtttg aatctaggtc tactgcctcc aaagccagtc ttctctcctg
     5521 caacatcatg ctctgtctag caggagatga gaacaggtct ccatttggag cctgtcagtg
     5581 gggtcagaga ctaagattca ggctcagggt ctaaattccg tatcctttct tccataccct
     5641 ggtgtttcct atgaacagat agatacttta gggctgcaag gtttggattg catggcactg
     5701 ctcagaagat aagttacagg tctgggctag gctgtagctg cccctccagg tggctagacc
     5761 tttcctttct gtgtcaccag ttaacactgg ccaacagttc cttccattaa ctgttcactg
     5821 ctttctcctg tgtctaactg atgcagttta tgacccataa ctaagagcag taccaggtat
     5881 ggctctgttt cctgttcatg tcccctgtcc tctgggctgc atgcattccg ttcttacaga
     5941 aagaatacct ttaacctagt acatcctgcc acacatctgc ttctactgtg aaattgatga
     6001 gggggtatta ccgattcttc cctctcccat catttactga gatgctggtg attgcattat
     6061 aatcctctta agcttacatt gtctttctga ttcttggtct tatctgagca agtgatctat
     6121 aaataactca gtggctttct catgactgtt ttaattatta gattttaatc aagtgtctta
     6181 ttaaatatat ctgcatgctt ccacaggcat ctgtctcttc acatggctgt tcagtgtgcc
     6241 tctcacaact tagcccaaac tcagttgagc tgccttgctt tggctttgac ccagctttcc
     6301 agcgctgctc aatctgttgc catggcaggc cattggaaag gctcagttca tccccgtgcc
     6361 tgaagccaag tgagcgctca ctccatgcat gcatggaggc tgggcaggag cctgcctaat
     6421 caaccagcca tgtgaggagg gagggcctgt tccttcctgt aagctatgtc atgaggcagc
     6481 gtggtcaagt cctctgccag ggagtggcct ggcccagcct gggcatgttt tcatgccagg
     6541 gtgctagagc ctactgccag attgtctccc tccaccccca atgaaaaaat ccttccagaa
     6601 gggaagagcc aatttcccct gtattggagg ggaagtggca gcacctcctg aagcagttgg
     6661 actttcatca ccctacctct gcatctgcct gaaggacaga tttagccaat taacctaagg
     6721 ttaccttcct ctctgataaa ttccccattc tgtcttccca tgtgttgtgt ctcgtttttt
     6781 tcctcctcct tccctcttcc ttgccccctc ttcccctaaa ccttacagat agctcgtgag
     6841 gctgaggcag ccatgttcca ccgcaagctg tttgaagaac ttgtgcgagc ctcaagtcac
     6901 tccacagacc tcatggaagc catggccatg ggcagcgtgg aggcttctta taagtgttta
     6961 gcagcagctt tgatagttct gacggagtct ggcaggtagg gccctaaggg caggtaacac
     7021 tgttaggata accagcctct tgctgcacct gccccaggag aagagagaag gcccaacctg
     7081 gcatctggga acagagcctc ttctcgtctg taggaacacc gccagggagg tcatggcagg
     7141 gcagaccaaa gggtcctgtg gctcagtagg cacagtagat gtcacaggca cttggtgaag
     7201 gactggtttc tgtggagtct tgatcttggc tcagctcaga atctccagtg attgggctcc
     7261 tcttggcctt tgttcccagg aacatgttcc tcaccagctg tccggtgact cttcccctcc
     7321 ctctcctttt gtgacaaagc tctgacaaag ctctgtcccc ctctcgtccc tctggacgga
     7381 tgttgctccc ctagattgcc cgtgaggcag aggctgccat ctaccacttg caattatttg
     7441 aggaactccg ccgcctggcg cccattacca gcgaccccac agaagccacc gccgtgggtg
     7501 ccgtggaggc ctccttcaag tgctgcagtg gggccataat cgtcctcacc aagtctggca
     7561 ggtaggaggc ggcagcggct ccctggaatg ccctgctcag tggtacctca ccttgggggt
     7621 cctgggagca gtccattgaa caatgctcag gtggcactga gccaaggtaa gacccctctg
     7681 cctgccacct tgggcctgca gggaaggatt gagcagagcc ccttcccagg gcccaaagga
     7741 ctctaggtag cactcataag gaatgtcaga acatttggat caaaagcaaa tttatgctgg
     7801 agatttatta cataacagtg cacaggctga ctacaaatgg ttatttgata ttgaaaattt
     7861 agtcctctaa aattgtaaaa gataccactt ttgcttattc cagttactat gtgctcttta
     7921 aaaatttcag ttgggaaatg aatttattta aatgctgttt actgtgcctc catttggcac
     7981 actagtccct gctgtttttg agccctaaag acaaattggg ttccagctca ggagaggttg
     8041 ctgtgctatc ttggctgaca ttctgtgggc ctggcagcca ggctgaggac tgtgtggcct
     8101 atgctgggcc tccaacttgg gatcccttcc ttggcccagg acattgagtt aatgtccttc
     8161 actctcctag ttagggagta tgctccttgt ccctgtccac aggggagcaa gggtttcctg
     8221 gaagagggga gcaaacaggc agtgcccatg cactgaggag cagcagatgg gcgtgggcag
     8281 cccagagaac caggacacaa gctctgtgca gatccctcag cagagggctc cagcctccca
     8341 ctcttggctg aacagctcca acccgtaggg ttgacctttc ttaaaaggtc cagttcttgc
     8401 tgtttggcta ttttaagctc tagtcttctg gggtttcact cagctggtcc tggcttcagc
     8461 aattgcttcc ctctgaaggc cttgcataga ggccaagcgt gaagtgcagg gacttctctg
     8521 ctgtgatgtg gcttaagttt ccctgacacc tgttgagtgt cctcataact tcccttctgg
     8581 tgcccctccc cagctcctga gaccagctgc agctacaagt gtgcagtgtc agtgttcaag
     8641 aaagtgcctg gcagaggggc tttagaaggg tcccctgcct tccaaaggag ctttggcagg
     8701 cagacgtgct cctgcagcaa cactcccatt tcctgttctt gcctgctgag tagcacctag
     8761 atttctaagc ctcatctaga tactcagatt tgattctggg cctttatagc ccagttgctg
     8821 ggactgtttc aggagctagg ggccatgtgg ggcagggaga gggcacaaaa gtagagaagc
     8881 ctgatgttga ttcccagggg gctggtcagc tctgctactg ctccttgcag atgtcaagag
     8941 tcaggtgcta gtcacgtgct gcttggcttg tcactgtcat tggcagcgag aggaatgggt
     9001 gctggtgaca ttgggccagg gctgcctctc tgtgtcagag ttcagggtgt aggaggggtt
     9061 ctgccaacca tgggctgtgt ggggtaagtg ggttgaggct gatctttctg ggtcaaggtg
     9121 atcctgagcc cttgcctgtg gaatgggggt agagggcaat ggtaacctag ctagcatgct
     9181 gtgggggata taggatgagg ggctgcccga ccctcgggag gggtcctagg gagcagatgt
     9241 tgaagaggcc agagccctca gtgagctgga tgagggggtg agccgtttga actccctgag
     9301 ggtacttcct ggggcctcgt gtaatggtct cttctgtatg tcccccatcc catctcaggt
     9361 ctgctcacca ggtggccaga taccgcccac gtgcccccat cattgctgtg acccggaatc
     9421 cccagacagc tcgtcaggcc cacctgtacc gtggcatctt ccctgtgctg tgcaaggacc
     9481 cagtccagga ggcctgggct gaggacgtgg acctccgggt gaactttgcc atgaatgttg
     9541 gtacgtggct ggagcagggg ctagagccta gaggagcttg gggatgcttg agcattggcc
     9601 accaacctcc cttctcttcc tccaggcaag gcccgaggct tcttcaagaa gggagatgtg
     9661 gtcattgtgc tgaccggatg gcgccctggc tccggcttca ccaacaccat gcgtgttgtt
     9721 cctgtgccgt gatggacccc agagcccctc ctccagcccc tgtcccaccc ccttccccca
     9781 gcccatccat taggccagca acgcttgtag aactcactct gggctgtaac gtggcactgg
     9841 taggttggga caccagggaa gaagatcaac gcctcactga aacatggctg tgtttgcagc
     9901 ctgctctagt gggacagccc agagcctggc tgccccatca tgtggcccca cccaatcaag
     9961 ggaagaagga ggaatgctgg actggaggcc cctggagcca gatggcaaga gggtgacagc
    10021 ttcctttcct gtgtgtactc tgtccagttc ctttagaaaa aatggatgcc cagaggactc
    10081 ccaaccctgg cttggggtca agaaacagcc agcaagagtt aggggtcctt agggcactgg
    10141 gctgttgttc cattgaagcc gactctggcc ctggccctta cttgcttctc tagctctcta
    10201 ggcctctcca gtttgcacct gtccccaccc tccactcagc tgtcctgcag caaacactcc
    10261 accctccacc ttccatttcc cccactactg cagcacctcc aggcctgttg ctatagagcc
    10321 tacctgtatg taataaacaa cagctgaagc acctgtttcc tctctttt
//