From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Escherichia coli pyridoxamine 5'-phosphate oxidase (pdxH) gene,
Date: 21 Dec 92 20:27:03 GMT
Distribution: bionet
Checksum: 63059 5

LOCUS       ECOTYSPDH    1142 bp ds-DNA             BCT       17-NOV-1992
DEFINITION  Escherichia coli pyridoxamine 5'-phosphate oxidase (pdxH) gene,
            complete cds, tyrosyl-tRNA synthetase (tyrS) gene, 3' end, ORF, 5'
            end.
ACCESSION   M92351
KEYWORDS    flavoprotein; operon; oxygen oxidoreductase; pdxH gene;
            pyridoxamine-phosphate oxidase; pyridoxine phosphate;
            transfer RNA-Tyr ligase; transfer RNA-Tyr synthetase; tyrS gene.
SOURCE      Escherichia coli (strain K-12) DNA.
  ORGANISM  Escherichia coli
            Prokaryotae; Gram-negative facultatively anaerobic rods;
            Enterobacteriaceae.
REFERENCE   1  (bases 1 to 1142)
  AUTHORS   Lam,H.-M. and Winkler,M.E.
  TITLE     Characterization of the complex pdxH-tyrS operon of Escherichia
            coli K-12 and pleiotropic phenotypes caused by pdxH insertion
            mutations
  JOURNAL   J. Bacteriol. 174, 6033-6045 (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     RBS             302..305
                     /gene="pdxH"
                     /citation=[1]
     protein_bind    907..921
                     /bound_moiety="putative FIS"
                     /note="putative"
                     /citation=[1]
     protein_bind    949..963
                     /bound_moiety="putative FIS"
                     /note="putative"
                     /citation=[1]
     RBS             1085..1088
                     /gene="tyrS"
                     /citation=[1]
     CDS             <1..251
                     /function="unknown"
                     /note="ORF"
                     /citation=[1]
                     /note="putative"
                     /codon_start=3
                     /translation="MQTDTLEYQCDEKPLTVKLNNPRQEVSFVYDNQLLHLKQGISAS
                     GARYTDGIYVFWSKGDEATVYKRDRIVLNNCQLQNPQR"
     CDS             310..966
                     /function="oxidize PNP and PMP into pyridoxal 5'-phosphate
                     (PLP)"
                     /EC_number="1.4.3.5"
                     /standard_name="Pyridoxamine 5'-phosphate oxidase"
                     /evidence="EXPERIMENTAL"
                     /citation=[1]
                     /gene="pdxH"
                     /product="pyridoxamine phosphate oxidase"
                     /codon_start=1
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHGQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     CDS             1095..>1142
                     /function="amino-acylation of tyr-tRNA with tyrosine"
                     /EC_number="6.1.1.1"
                     /note="putative"
                     /citation=[1]
                     /gene="tyrS"
                     /product="transfer RNA-Tyr synthetase"
                     /codon_start=1
                     /translation="MASSNLIKQLQERGLV"
BASE COUNT      294 a    275 c    288 g    285 t
ORIGIN      
        1 gcatgcaaac cgatacgctg gaataccagt gtgatgaaaa accgttgacg gtcaaactga
       61 ataatccgcg ccaggaggtc agttttgttt acgataatca actactgcat ctcaaacagg
      121 gcatttcagc ctctggcgcg cgttacactg acggaatcta tgttttctgg tcgaaaggcg
      181 atgaagcgac tgtctataaa cgcgaccgca tcgtcttgaa taactgtcag ttacaaaatc
      241 cacagcgttg agatttttcc aggggcggcg cacaatagcg tcacccactg acaatccgta
      301 aagaaaacca tgtctgataa cgacgaattg cagcaaatcg cgcatctgcg ccgtgaatac
      361 accaaaggcg ggttacgccg ccgcgatctt cccgccgatc cattaaccct ttttgaacgt
      421 tggctctctc aggcttgtga agccaaactg gcggacccta ccgcgatggt ggtcgctacc
      481 gtggatgaac atggtcagcc ttatcagcgc atcgttttac tcaaacatta cgacgaaaaa
      541 ggcatggtgt tttacaccaa cctcggcagc cgtaaagcac atcaaatcga aaataatccg
      601 cgcgttagcc tgctgttccc gtggcatacc cttgagcgcc aggtgatggt gatcggtaaa
      661 gcagaacgac tttcgactct cgaagtgatg aaatattttc atagccgccc gcgtgatagc
      721 cagattggtg catgggtttc gaagcagtcc agtcgcattt ctgcccgcgg tatccttgaa
      781 agtaaattcc tggagctgaa gcagaagttt caacagggcg aagtgccatt gccgagcttt
      841 tggggcggtt ttcgcgtcag ccttgaacag attgagttct ggcagggtgg tgagcatcgc
      901 ctgcatgacc gctttttgta ccagcgtgaa aatgatgcgt ggaagattga tcgtcttgca
      961 ccctgaaaag atgcaaaaat cttgctttaa tcgctggtac tcctgattct ggcactttat
     1021 tctatgtctc tttcgcatct ggcgaaaagt cgtgtaccgg caaaggtgca gtcgttatat
     1081 acatggagat tttgatggca agcagtaact tgattaaaca attgcaagag cgggggctgg
     1141 ta
//