From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: E.coli malI, malX, and malY genes, complete cds.
Date: 15 Sep 92 10:02:57 GMT
Distribution: bionet
Checksum: 01205    10

LOCUS       ECOMALAA     4202 bp ss-mRNA            BCT       15-SEP-1992
DEFINITION  E.coli malI, malX, and malY genes, complete cds.
ACCESSION   M60722
KEYWORDS    malI gene; malX gene; malY gene;
            phosphotransferase system enzyme II; repressor protein.
SOURCE      E.coli, cDNA to mRNA.
  ORGANISM  Escherichia coli
            Prokaryotae; Gram-negative facultatively anaerobic rods;
            Enterobacteriaceae.
REFERENCE   1  (bases 1 to 4202)
  AUTHORS   Reidl,J. and Boos,W.
  TITLE     The malX malY operon of Escherichia coli encodes a novel enzyme II
            of the phosphotransferase system recognizing glucose and maltose
            and an enzyme abolishing the endogenous induction of the maltose
            system
  JOURNAL   J. Bacteriol. 173, 4862-4876 (1991)
  STANDARD  simple staff_entry
FEATURES             Location/Qualifiers
     CDS             complement(261..1238)
                     /function="constitutive expression"
                     /product="repressor protein"
                     /gene="malI"
                     /codon_start=1
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAIYRCDTNHTCMHIPVTWASTPARELGITLADSHDAKNHP"
     protein_bind    1271..1282
                     /bound_moiety="MalI repressor"
                     /note="putative"
     -35_signal      1279..1284
                     /gene="mal"
     mRNA            1291..4202
                     /gene="mal"
                     /partial
     -10_signal      1306..1311
                     /gene="mal"
     protein_bind    1319..1339
                     /bound_moiety="CAP"
                     /gene="mal"
     protein_bind    1349..1360
                     /bound_moiety="MalI repressor"
                     /note="putative"
     RBS             1401..1405
                     /gene="mal"
     CDS             1413..3005
                     /product="phosphotransferase system enzyme II"
                     /gene="malX"
                     /codon_start=1
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     IGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSYDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESATRFLSQGKMNAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSRVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVKDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     CDS             3015..4187
                     /function="degrades the inducer of the maltose system or
                     prevents its synthesis"
                     /gene="malY"
                     /codon_start=1
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDSQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHGVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRIYLKDNLTYIADKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
BASE COUNT      957 a   1095 c   1061 g   1089 t
ORIGIN
        1 ccgaatagtc aatggcgatt tctttaccaa tacctgcacc cgcacctgta tgatggcgca
       61 tttccgtcga gtctcaggtt gtcagaatta aacacataaa cctcctgtga acttcagtaa
      121 aaataaaagg caccgcctgt gtgcacattt tttatcatta accttgagga aaggacacta
      181 ccaggtatag cagataaaga gaggattatt tcgctgcaat gagccgggcg ggaataataa
      241 gattgcgtga atgcgtctct tcatgggtga ttttttgcat catgcgagtc cgcaagtgtg
      301 ataccaagtt cccgcgctgg cgtgctggcc caggtaacag gtatatgcat gcaagtgtgg
      361 ttggtgtcgc atcggtaaat agccgccagc gaaacctgtt gctcaaagta acgatcgact
      421 ccgctttccc cgctttgccg ccctgctttg agcaaaccaa accatgcccc catcgcaata
      481 gtttcgttat agcaaaccac ggcactgatg gtcgggttat gacgtaaaag cgccgtgata
      541 gcttccgcgg cttgcttctg gctggaagtg cactccaaca cccaatcgct atgaaacggc
      601 aggccaaatt ttagtagagt tgcacaatag cccccaaccc gttctgcacg ggttaatgag
      661 gaactttgcc ctcccagcca ggcgatccgc tgatgcccat tgcgaatgag atgctccgtc
      721 aacaactgtg cagcctgcat gttgtccggg cgaaccgtat caacatcatc aagataactg
      781 gcacgggaag cgaaaatcac cgggatagct ttttcttctg ccatccgtcg caggtcatcg
      841 ctacttcctg cagctccggc aattaccaca ccatcgacac cctgattcag taacagtgaa
      901 aaccgctgtg ccagctgctc accgtcttta ccgccgtgaa gcaaaaaaac catccgtccc
      961 tgcgcttcca gagcttccgt caatccggcc gtcaattcgg cgtaaaacgg cgcagataaa
     1021 tcacggacga tcaaaccaat gacgccgctt tgcccgccgc gcagcgccga cgcctggcga
     1081 ttgcgcacaa atcccagctc ttcaatggcg gcgttaacgc gttctcctgt ggcggtagag
     1141 attcgccctt tgccactaag caccagcgaa acggtactta ccgacacgcc cgcagccagc
     1201 gcaacatcat gaatggttat ttttttggcg gtagccatgc agatgaccta ctccctgatt
     1261 atgtgacaga taaaacgttt taccttttat ttatcttata cccgctatta tcgttgcgta
     1321 atgtgattta tgcctcacta aaatttgata aaacgtttta tcttctcgcg caatttactg
     1381 aatccagatt gttctctacg aggagtcgtt ttatgacggc gaaaacagca ccgaaagtca
     1441 cgctgtggga gttcttccag cagttaggca aaaccttcat gttacccgtg gcattattgt
     1501 cgttctgcgg cattatgctc ggcattggta gttctcttag cagccatgat gtcataaccc
     1561 tgatcccggt cctgggcaac cccgtgttgc aggctatctt tacctggatg agtaagattg
     1621 gctcgtttgc ttttagtttc ctgcctgtca tgttctgtat cgccatcccg ctgggcctgg
     1681 cacgcgaaaa taaaggcgta gcggcattcg ctggcttcat cggttatgcg gtaatgaacc
     1741 tcgcggtaaa cttctggttg accaataaag gcattctgcc aaccacggat gccgcggttc
     1801 tgaaagccaa taacatccag agcattcttg ggatccagtc gtacgatacc gggatcctcg
     1861 gtgcggtgat cgccggtatt atcgtctgga tgctgcatga gcgtttccat aatatccgcc
     1921 tgccggatgc gctggcattc ttcggcggta cgcgcttcgt accaattatc tcctcgctgg
     1981 tgatgggcct tgtcggcctg gtgattccat tagtctggcc gattttcgcc atgggtatta
     2041 gcggcttggg ccatatgata aacagcgcgg gtgatttcgg accgatgctg tttggtaccg
     2101 gtgaacgtct gctgttgccg tttggtctgc atcacattct ggtggcatta attcgcttta
     2161 ccgacgcagg cggcacgcag gaagtctgcg gtcaaaccgt cagcggcgca ctgaccatct
     2221 tccaggcgca attgagttgc ccgaccactc acggtttttc tgaaagcgcc acgcgtttcc
     2281 tttcgcaagg taaaatgaat gcgtttctcg gcggtctgcc aggtgcagcg ttagctatgt
     2341 atcactgcgc gcgcccggaa aatcgccata aaattaaagg tctgctgatt tctggcctga
     2401 tcgcctgcgt cgttggcggc actaccgaac cgctggaatt cctgttcctg ttcgtagcgc
     2461 cagttctgta tgtcatccac gcgctgttaa ccggcctcgg cttcaccgtc atgtctgtgc
     2521 tcggcgtcac catcggtaat accgacggca atatcatcga cttcgtggtg ttcggtattt
     2581 tgcatggtct gtcaaccaag tggtacatgg tgccagtggt ggcggcaatc tggtttgtcg
     2641 tttactacgt catcttccgt ttcgctatca cccgcttcaa tctgaaaacc ccggggcgcg
     2701 atagcagagt tgccagctca atcgaaaaag ccgttgccgg tgcgccgggt aaatcaggtt
     2761 acaacgttcc tgcaatcctc gaagcattag gcggtgccga caatattgtc agcctcgata
     2821 actgcattac ccgtctgcgt ttgtctgtga aagatatgtc gcttgttaat gtgcaggcac
     2881 tgaaggacaa tcgggcaatt ggcgtagtac aacttaatca acataacctg caggttgtta
     2941 tcgggccaca agttcagtca gtaaaagatg aaatggccgg tctgatgcat actgtccagg
     3001 cataaggata agatatgttc gatttttcaa aggtcgtgga tcgtcatggc acatggtgta
     3061 cacagtggga ttatgtcgct gaccgtttcg gcactgctga cctgttaccg ttcacgattt
     3121 cagacatgga ttttgccact gccccctgca ttatcgaggc gctgaatcag cgcctgatgc
     3181 acggcgtatt tggctacagc cgctggaaaa acgatgagtt tctcgcggct attgcccact
     3241 ggttttccac ccagcattac accgccatcg attctcagac ggtggtgtat ggcccttctg
     3301 tcatctatat ggtttcagaa ctgattcgtc agtggtctga aacaggtgaa ggcgtggtga
     3361 tccacacacc cgcctatgac gcattttaca aggccattga aggtaaccag cgcacagtaa
     3421 tgcccgttgc tttagagaag caggctgatg gttggttttg cgatatgggc aagttggaag
     3481 ccgtgttggc gaaaccagaa tgtaaaatta tgctcctgtg tagcccacag aatcctaccg
     3541 ggaaagtgtg gacgtgcgat gagctggaga tcatggctga cctgtgcgag cgtcatggtg
     3601 tgcgggttat ttccgatgaa atccatatgg atatggtttg gggcgagcag ccgcatattc
     3661 cctggagtaa tgtggctcgc ggagactggg cgttgctaac gtcgggctcg aaaagtttca
     3721 atattcccgc cctgaccggt gcttacggga ttatagaaaa tagcagtagc cgcgatgcct
     3781 atttatcggc actgaaaggc cgtgatgggc tttcttcccc ttcggtactg gcgttaactg
     3841 cccatatcgc cgcctatcag caaggcgcgc cgtggctgga tgccttacgc atctatctga
     3901 aagataacct gacgtatatc gcagataaaa tgaacgccgc gtttcctgaa ctcaactggc
     3961 agatcccaca atccacttat ctggcatggc ttgatttacg tccgttgaat attgacgaca
     4021 acgcgttgca aaaagcactt atcgaacaag aaaaagtcgc gatcatgccg gggtatacct
     4081 acggtgaaga aggtcgtggt tttgtccgtc tcaatgccgg ctgcccacgt tcgaaactgg
     4141 aaaaaggtgt ggctggatta attaacgcca tccgcgctgt tcgttaaccc caattgcgta
     4201 cc
//