From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Bacillus subtilis ATP-dependent nuclease (addA) and (addB), and
Date: 22 Jan 93 19:46:18 GMT
Distribution: bionet
Checksum: 25145 16

LOCUS       BACADDAA     8732 bp ds-DNA             BCT       21-JAN-1993
DEFINITION  Bacillus subtilis ATP-dependent nuclease (addA) and (addB), and
            open reading frame 3, partial cds.
ACCESSION   M63489
KEYWORDS    ATP-dependent nuclease.
SOURCE      B.subtilis DNA.
  ORGANISM  Bacillus subtilis
            Prokaryotae; Firmicutes; Endospore-forming rods and cocci;
            Bacillaceae.
REFERENCE   1  (bases 1 to 8732)
  AUTHORS   Kooistra,J. and Venema,G.
  TITLE     Cloning, sequencing, and expression of bacillus subtillis genes
            involved in ATP-dependent nuclease synthesis
  JOURNAL   J. Bacteriol. 173, 3644-3655 (1991)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     CDS             502..4002
                     /gene="addA"
                     /product="ATP-dependent nuclease"
                     /codon_start=1
                     /translation="MGAEFLVGRSGSGKTKLIINSIQDELRRAPFGKPIIFLVPDQMT
                     FLMEYELAKTPDMGGMIRAQVFSFSRLAWRVLQHTGGMSRPFLTSTGVQMLLRKLIEE
                     HKQEFKVYQKASDKSGFTAQVERMLTEFKRYCLEPEDIRRMAESGTASEYRGERVLSE
                     KLHDLSILYQQMEKSLADQYLHSEDYLTLLAEHIPLAEDIKGAHIYVDGFYQFTPQEF
                     RVLEQLMVHAEHITFSLTADKPSYEREPHELELFRMTGKTYYRLHQKAKELNLDITYK
                     ELSGTERHTKTPELAHLEAQYEARPAIPYAEKQEALTVMQAANRRAELEGIAREIHAL
                     VREKGYRYKDVAILARQPEDYKDMVKEVFADYEIPYFIDGKASMLNHPLIEFIRSSLD
                     VLKGNWRYEAVFRCVKTELLFPLNEPKAKVREQVDQLENYCIAYGIKGDRWTKGDRFQ
                     YRRFVSLDDDFAQTDQEIEMENMLNDTRDWIVPPLFQLQKRMKKAKTVQEKAEALYRY
                     LEETDVPLKLDQERQRAEDDGRIIEAQQHQQAWDAVIQLLEEFVEMMGDDEISLDLFQ
                     QMIEAGAESLTFSLIPPALDQVFVGNMDLSRMYGTSCTFVLGANDGVLPARPDENGVL
                     SDDDREWLKTIGVELSSGGRERLLDEHFLIYMAFSSPSDRLYVSYPIADAEGKTLLPS
                     MIVKRLEELFPHHKERLLTNEPEQVSDEEQLMYVVNKSVAQSFTASQLRLWTREYDIS
                     DVWWSTYNVLMSEQDRLQSKKLFSSLFFRNEVKQLERSVSRQLYGERIQGSVSRMETF
                     NACPFSHFASHGLHLKERQFFKLEAPDIGQLFHSSLKLISDRLRDEKLDWRDLTKEQC
                     ELFSYDAVERLAPKLQKEILLSSNRHYYVKEKLQKIVTRVSGILSEHAKASGFVPIGL
                     ELGFGGKGPLPPLTFQLKNGCTMELVGRIDRVDKAESSKGLLLRIVDYKSSDKGLDLA
                     EVYYGLALQMLTYLDLSITHSADWLGMRATPAGVLYFHIHDPMIQSNLPLGLDEIEQE
                     IFKKFKMKGLLLGDQEVVRLMDTTLQEGRSNIINAGLKKDGSLRSDSAAVGEKEFDLL
                     TKHVRRTFQEAGEQITDGRVSIEPYKMKNKTPCTYCAFKSVCQFDESLEENEYRPLKA
                     EKDKTILEWIKKEADGNEHS"
     CDS             3988..7687
                     /gene="addB"
                     /product="ATP-dependent nuclease"
                     /codon_start=2
                     /translation="MNIPKPADSTWTDDQWNAIVSTGQDILVAAAAGSGKTAVLVERM
                     IRKITAEENPIDVDRLLVVTFTNASAAEMKHRIAEALEKELVQRPGSLHIRRQLSLLN
                     RASISTLHSFCLQVLKKYYYLIDLDPGFRIADQTEGELIGDEVLDELFEDEYAKGEKA
                     FFELVDRYTTDRHDLDLQFLVKQVYEYSRSHPNPEAWLESFVHLYDVSEKSAIEELPF
                     YQYVKEDIAMVLNGAKEKLLRALELTKAPGGPAPRADNFLDDLAQIDELIQHQDDFSE
                     LYKRVPAVSFKRAKAVKGDEFDPALLDEATDLRNGAKKLLEKLKTDYFTRSPEQHLKS
                     LAEMKPVIETLVQLVISYGKRFEAAKQEKSIIDFSDLEHYCLAILTAENDKGEREPSE
                     AARFYQEQFHEVLVDEYQDTNLVQESILQLVTSGPEETGNLFMVGDVKQSIYRFRLAE
                     PLLFLSKYKRFTESGEGTGRKIDLNKNFRSRADILDSTNFLFKQLMGGKIGEVDYDEQ
                     AELKLGAAYPDNDETETELLLIDNAEDTDASEEAEELETVQFEAKAIAKEIRKLISSP
                     FKVYDGKKKTHRNIQYRDIVILLRSMPWAPQIMEELRAQGIPVYANLTSGYFEAVEVA
                     VALSVLKVIDNPYQDIPLASVLRSPIVGADENELSLIRLENKKAPYYEAMKDYLAAGD
                     RSDELYQKLNTFYGHLQKWRAFSKNHSVSELIWEVYRDTKYMDYVGGMPGGKQRQANL
                     RVLYDRARQYESTAFRGLFRFLRFIERMQERGDDLGTARGLSEQEDVVRLMTIHSSKG
                     LEFPVVFVAGLGRNFNMMDLNKSYLLDKELGFGTKYIHPQLRISYPTLPLIAMKKKMR
                     RELLSEELRVLYVALTRAKEKLFLIGSCKDHQKQLAKWQASASQTDWLLPEFDRYQAR
                     TYLDFIGPALARHRDLGDLAGVPAHADISGHPARFAVQMIHSYDLLDDDLEERMEEKS
                     ERLEAIRRGEPVPGSFAFDEKAREQLSWTYPHQEVTQIRTKQSVSEIKRKREYEDEYS
                     GRAPVKPADGSILYRRPAFMMKKGLTAAEKGTAMHTVMQHIPLSHVPSIEEAEQTVHR
                     LYEKELLTEEQKDAIDIEEIVQFFHTEIGGQLIGAKWKDREIPFSLALPAKEIYPDAH
                     EADEPLLVQGIIDCLYETEDGLYLLDYKSDRIEGKFQHGFEGAAPILKKRYETQIQLY
                     TKAVEQIAKTKVKGCALYFFDGGHILTL"
     CDS             7759..8520
                     /note="putative;  ORF 3"
                     /codon_start=1
                     /translation="MRILHTADWHLGKTLEGRSRLSEQADVLDELNTIVKDEQIDAIV
                     MAGDAFDTVNPPALAEQLFYESLSALSDRGKRPIVVIAGNHDNPDRLSAASPLTHENG
                     IHLIGYPTTEPIHIEVPSAGELLAVGALAYPSEARLNEVLSDTFDEKLLRDHYDVKIR
                     QAFEHMTSRFRTDAVKIAASHIYVAGETKPIQTGRLKSAVHTRWLLKACRQMPLTLRS
                     AICIARKRSSGRGRLRVIQDLRSPTAFLKRATLNQ"
BASE COUNT     2387 a   1877 c   2406 g   2062 t
ORIGIN      
        1 gtcgacggtt tcttgttcat ctttaaaagc ggcgtccagt cctcttgtga aattgtccgg
       61 gcattcttcc gtttgtttcg gcatgatgtg tttgccggta attgaaccat acagtgtgga
      121 aaattcacgg aggtggcgaa tctcgtcacg cctgatttct tcaatctgtt ttctgacctc
      181 atcgctgtgg gcgagttcgg caagcttgcg gtagcattga atagcactga attctccgtt
      241 gatggccttc tgcaaattcc gaatcaaaac aggatcttcc cggtatggca ccggataggg
      301 atagtagctg taatgcaagg ggattcaact ccattcttag gaattgggct tctgtaaaag
      361 tatatgttgg gcagggagag atgtgcggag ataatcagct ttttatatgt gaaaaggccg
      421 tttttaccaa tagatcagat tggtcatttt cgtcaacatt cgataaaata tagagagata
      481 aaaagagagg ggtcttctaa tttgggagca gagtttttag taggcaggtc cgggagtgga
      541 aaaacgaagc tgatcatcaa cagcattcag gatgaattgc gccgggctcc attcgggaag
      601 ccgatcattt ttctagtccc ggatcaaatg acgtttttaa tggaatacga gcttgctaaa
      661 acgccagata tgggcgggat gatacgcgct caagtgttca gtttttcacg attggcctgg
      721 cgcgtcctcc agcatacggg aggaatgagc aggccgtttc tgacgagcac cggcgtacaa
      781 atgctcctgc ggaagctcat tgaggagcat aaacaggagt tcaaagtcta tcaaaaagcg
      841 agtgacaaaa gcgggtttac cgcacaggtt gagcggatgc tgacagagtt taagcgctat
      901 tgtctggaac cggaggatat ccgccggatg gcggaaagcg gaacggcttc cgagtatcgc
      961 ggagaacgtg ttttatctga aaagctccat gacttatcaa ttctgtatca gcaaatggaa
     1021 aaaagcctcg cagatcaata tcttcactct gaggattatt tgacattgct ggcagagcat
     1081 atcccgcttg cggaagatat aaaaggcgcc catatctatg tggatggctt ttatcagttt
     1141 accccgcagg aattcagggt gttggagcag cttatggttc atgcggagca tatcacgttt
     1201 tcgctcacag cggacaagcc gtcatatgag cgggagccgc atgaactgga attgttcaga
     1261 atgacgggga aaacctatta ccgcctgcat cagaaggcga aggaactgaa tttggacatt
     1321 acgtataaag agctgagcgg gactgagcgg catacaaaga cgccggaatt ggcgcatcta
     1381 gaggcacagt atgaagcgcg tccggccatt ccatacgcag aaaaacaaga agcccttact
     1441 gtgatgcagg ccgcaaacag acgagctgag ctggaaggca ttgctcggga aattcacgcc
     1501 ctagtcagag agaagggata tcgctataag gatgtagcaa ttcttgcgcg ccagccggaa
     1561 gactacaagg atatggtgaa ggaagttttc gcagattacg agattcctta tttcattgac
     1621 ggaaaagcat ctatgctgaa ccatccgtta attgaattta tccggtcgag ccttgatgtt
     1681 ctgaaaggga attggcgtta tgaagcggtg tttcgctgcg tgaaaaccga actgctattc
     1741 ccgctcaatg aaccgaaggc caaagtgaga gaacaggtcg atcagctcga aaattactgt
     1801 atcgcctatg gtattaaagg cgaccgctgg acaaagggcg atcggttcca atacaggcgt
     1861 tttgtgtcat tggatgatga ttttgcgcag actgatcagg aaatcgaaat ggaaaacatg
     1921 ttgaatgaca cccgcgattg gattgttccg ccgctttttc agcttcaaaa acgcatgaaa
     1981 aaagcgaaga cggttcaaga gaaggcggag gcgctctatc gttatttaga agagacggat
     2041 gtgccgctga agctggatca ggaaaggcag cgtgctgagg atgacggcag aatcattgaa
     2101 gcgcagcagc atcagcaggc gtgggacgca gtcattcagc tgcttgagga gtttgttgaa
     2161 atgatgggag atgatgagat ttctcttgat ttgtttcagc aaatgataga agccggcgcg
     2221 gagtcgctta cattttctct aattccgcct gcgcttgacc aggtgtttgt cggcaatatg
     2281 gatttgtcca gaatgtatgg cacctcctgc acctttgtgc tcggggcaaa cgacggcgtt
     2341 ctgccggcac gccctgatga aaacggggtc ctgtcggatg atgaccggga atggctgaaa
     2401 acgattgggg ttgagctatc ctcaggcgga cgagagcgtt tgcttgatga gcacttcctc
     2461 atctacatgg cgttttcaag tccgtctgac cggctttacg tatcgtatcc gattgctgat
     2521 gcggaaggaa aaacgctttt gccgtcgatg atcgttaagc ggctggaaga actgtttccg
     2581 catcataagg agcgcctgtt aacaaatgaa cctgaacagg tcagcgatga ggaacagctg
     2641 atgtatgttg tgaataaaag cgtggcacag tcctttaccg cgagccagct caggttatgg
     2701 actcgggaat acgacatcag cgacgtctgg tggagcacgt acaatgtgct gatgagtgag
     2761 caggacaggc tgcaatcgaa aaagctgttc tcaagcctgt ttttccggaa tgaagtgaag
     2821 cagcttgaac gcagcgtgtc gagacagctc tatggtgaac gtattcaggg cagtgtatcg
     2881 agaatggaaa cctttaacgc atgcccgttt tcccattttg cgtcacacgg gctgcatttg
     2941 aaggaacggc aattcttcaa gcttgaagca ccggatatcg gccagctgtt tcattccagc
     3001 ttaaagctga tttcggacag gctgcgtgac gaaaagctgg attggcgcga tttaacgaag
     3061 gagcagtgcg agctgttttc ctatgatgcg gtagagcggc tggcaccgaa actgcaaaag
     3121 gaaattctgc tcagctcaaa ccggcattat tatgtgaagg aaaaactgca aaaaattgtg
     3181 acacgtgtgt ccggcatttt aagcgagcat gcgaaagcga gcggattcgt accgatcggg
     3241 cttgaactgg gctttggagg aaaagggccg cttccgccgc tgacctttca actgaaaaac
     3301 ggctgtacga tggaactcgt cgggcgaatt gaccgtgttg ataaggctga aagctcaaaa
     3361 ggcctgctcc tcaggattgt cgattataaa tcaagcgaca aaggccttga cttagcggaa
     3421 gtatattacg gattggcact gcaaatgctg acgtaccttg atttatcgat tacacattca
     3481 gctgactggc tcgggatgag ggcgacgcct gccggagtgc tgtatttcca tattcatgac
     3541 ccgatgattc aatctaatct cccgcttggg cttgacgaga ttgaacagga gatctttaag
     3601 aaatttaaaa tgaagggctt gctcctcggt gatcaggaag tggttcgcct catggataca
     3661 acccttcaag agggacgttc aaatatcata aacgccggct tgaaaaaaga cggctctctc
     3721 agatcagact cagcagcagt cggtgaaaag gaatttgatc ttttgacaaa gcatgtgcgc
     3781 cgcaccttcc aagaagcggg cgaacaaatc accgacgggc gcgtatccat tgagccgtac
     3841 aaaatgaaga acaagacgcc gtgcacatac tgtgcgttca aatcagtatg ccaatttgat
     3901 gaatcactgg aagaaaacga gtatcgccca ttaaaggctg aaaaggacaa gacaatactt
     3961 gagtggataa aaaaggaggc ggatggcaat gaacattcct aaaccggcag acagcacatg
     4021 gacagatgac caatggaatg ccattgtttc aaccggccag gatattcttg tggcagcggc
     4081 tgcgggctct ggtaaaaccg ctgtgctcgt tgaacgaatg attcggaaaa tcaccgcgga
     4141 ggaaaaccca atagatgtag accgtcttct cgttgtgaca tttacaaacg cctcagcggc
     4201 agagatgaag caccgaatcg cagaagcctt ggaaaaagag cttgtacagc gccccggctc
     4261 gctgcatatt agacgccagc tgtctctttt aaaccgggcc agcatttcga cgctccattc
     4321 cttttgcctg caagtgctga agaaatatta ctacttgatc gatcttgatc cgggctttcg
     4381 gatagctgat cagacggaag gcgagctgat cggggatgaa gtgcttgacg agctgtttga
     4441 agacgaatac gcaaagggcg aaaaggcgtt ttttgagctt gttgaccgct atacgacaga
     4501 ccggcatgat ctggatctgc aatttctcgt taaacaggtg tacgagtatt cccgatccca
     4561 tcccaacccg gaggcgtggc tggaaagctt tgttcatttg tatgatgtat cagaaaagag
     4621 cgccatcgag gagctgccgt tttatcaata tgtcaaagaa gatattgcaa tggtgcttaa
     4681 cggggcgaag gaaaagctct tgcgcgcgct tgagctgacg aaagcgccgg gcggcccggc
     4741 gccgcgtgct gacaattttc ttgatgatct tgctcagatt gatgaactga ttcagcatca
     4801 ggacgatttc agtgaactat ataagcgggt gcccgccgtc tcttttaagc gtgccaaagc
     4861 agtaaaaggg gatgagttcg atccagcgct ccttgatgag gcgacagatt tgaggaacgg
     4921 cgcaaaaaaa ctgcttgaaa agctcaaaac cgactacttc acgcgaagtc ctgaacagca
     4981 cttgaaaagc ctagccgaga tgaagcctgt cattgaaacg ctcgtacagc ttgtcatcag
     5041 ctatggaaaa cgattcgaag ctgcgaaaca ggaaaaatca atcatcgatt tttcggattt
     5101 ggagcattac tgtttagcga ttttgacagc tgagaatgac aaaggtgaac gtgagccgag
     5161 cgaggctgca aggttttatc aggaacagtt tcatgaggtg ctcgttgacg aatatcagga
     5221 taccaacctc gtgcaggaat cgattctgca gctcgtcaca agcggtccgg aggagactgg
     5281 taacctgttt atggtaggag atgtcaaaca gtcgatttat cgattcaggc ttgcggagcc
     5341 gcttcttttc ctctctaaat acaaacggtt tacagagagc ggagaaggca cggggcggaa
     5401 aatcgattta aataaaaatt tccgaagccg ggctgatatt ttagacagca caaacttttt
     5461 atttaaacag ctgatgggcg gcaaaatcgg tgaggtcgat tatgacgagc aggctgagct
     5521 gaagcttggt gcagcgtatc cggacaatga cgagacggaa acagagctgc tgctgatcga
     5581 caacgcagaa gatacggatg caagcgagga agcagaagag cttgaaacgg tgcagtttga
     5641 ggcaaaagcc atcgctaagg aaattcgtaa gctgatttca tcgccgttta aggtgtatga
     5701 cggaaaaaag aaaacacatc gcaatattca ataccgagat atcgtgattt tgctccgttc
     5761 gatgccgtgg gctccgcaaa tcatggagga gctgagagca cagggcatac cggtttacgc
     5821 caatttaacg tcaggctatt ttgaagcggt cgaagtcgcc gtcgcgcttt ctgtgctgaa
     5881 ggtgattgat aatccgtatc aggatatacc gcttgcctct gtgctgcgct caccgattgt
     5941 cggagcagat gaaaacgagc tgtctttgat ccggcttgaa aataaaaaag cgccgtacta
     6001 tgaggcgatg aaagactacc tggctgctgg tgaccggagc gatgagcttt atcaaaagtt
     6061 aaatacgttt tacggacatc tgcaaaaatg gcgcgcgttt tcgaaaaacc actcagtatc
     6121 tgagctgatt tgggaagtgt accgcgacac caaatatatg gattatgtcg gcggcatgcc
     6181 gggcggaaaa cagcgccagg ccaatttgcg tgttctttat gacagggcgc gtcaatatga
     6241 atcaacggca tttcgcggct tgttccgttt cctgcggttt atcgaacgca tgcaggagcg
     6301 gggcgatgat cttggtacgg cgagaggcct cagcgagcag gaggatgttg tccgcttaat
     6361 gacgatccac agcagcaaag ggctcgaatt tccagtcgtg tttgtagcag gtctcggccg
     6421 gaatttcaac atgatggatt tgaacaaatc gtacctgctg gataaggagc tcggatttgg
     6481 cacgaagtat attcatccgc aattacgcat cagctatccg acacttccgc tcattgcgat
     6541 gaagaaaaaa atgcgcaggg agctgctgtc agaggaattg cgtgtgctct atgttgcatt
     6601 aacgagagcg aaggaaaagc tgtttctgat tggctcatgt aaggatcatc agaaacagct
     6661 tgcaaaatgg caggcatccg cgtcccaaac tgattggctt ctgccggaat ttgaccgcta
     6721 tcaggcgaga acgtatctag atttcattgg gccggctctt gccaggcaca gagacttggg
     6781 ggatttggct ggtgtgccag cacacgctga catctcaggt cacccggctc gttttgccgt
     6841 tcaaatgatc cattcctatg atttgcttga tgatgatctg gaagaaagaa tggaagaaaa
     6901 aagcgagcgc ctagaagcga tccgccgagg tgaaccagtt cccggctcgt ttgcgtttga
     6961 tgaaaaagcc cgcgagcagc tgagctggac ctacccgcat caagaagtga cgcaaattcg
     7021 gacaaagcaa tcagtttctg agatcaagag aaaaagagag tacgaggatg aatacagcgg
     7081 cagggcccct gtaaaaccgg ctgatggaag cattctgtac agacgtcccg cttttatgat
     7141 gaaaaaaggc ctgacagcgg cagagaaagg gactgccatg catacggtta tgcagcatat
     7201 cccgctgtca catgtgccgt cgatagaaga agctgagcag acggttcaca ggctttatga
     7261 aaaagagctt ctcactgaag aacaaaaaga cgctattgat atagaagaaa tcgtgcaatt
     7321 tttccataca gaaatcggcg gacagctgat cggtgctaag tggaaggacc gggaaatacc
     7381 attcagctta gcgcttccgg ccaaggagat ctatcctgat gcacacgagg cagatgagcc
     7441 gcttttagtg cagggtatta ttgactgtct ctatgaaact gaggacggat tatatctatt
     7501 ggattataag tcggaccgga ttgagggcaa attccagcat ggatttgaag gagcggcccc
     7561 gatcttgaag aaacgatatg aaacgcaaat tcagctgtac acgaaggcag tggagcaaat
     7621 tgcaaaaaca aaggtaaagg gatgtgcgct ttatttcttt gacggagggc acattctgac
     7681 attatagcga gatccataag ctccggaatt tcaggcggag cgggtctcgt tttctatata
     7741 aaagagaggt gaaagcattt gcggatttta catacggctg actggcatct tggaaaaacg
     7801 cttgaaggaa gaagcaggct gagtgaacag gccgatgtgc tggatgaact gaatacgatt
     7861 gtaaaggatg agcagatcga tgccattgta atggcggggg atgcatttga caccgtaaac
     7921 ccgccagctt tagccgagca gctgttttat gaaagcctgt ctgcgcttag cgacagagga
     7981 aagcgcccga tcgtcgtcat tgccggaaat cacgataatc ctgaccgttt gtccgccgct
     8041 tcaccgctga cacatgaaaa cggcattcat ttaatcggtt atccgacaac agagccgatt
     8101 catattgaag tgccttcagc aggagagctt ttggcggttg gagcgctggc gtacccatct
     8161 gaagcgcggt taaatgaagt gctatccgat acgtttgatg aaaagctgct ccgtgatcac
     8221 tatgatgtga aaatcaggca ggcgtttgag catatgacaa gccggttccg gaccgatgca
     8281 gtgaagattg ccgccagcca tatttatgtg gcaggcgaaa ccaaaccgat tcagacgggc
     8341 cgattgaagt cggcggtgca tacacggtgg ctgctgaaag cctgccggca gatgccgctt
     8401 acgttgcgct cggccatttg catcgcccgc aaacgatcaa gcgggcgcgg acgcttgcgc
     8461 gttattcagg atctccgctc gcctacagct tttctgaagc gggctacgct aaatcagtga
     8521 cgattgtgga cgcaaagcct ggggaagagg ccacttggca agaggtgtta ttatcaagcg
     8581 gcaagccact tgtgaagtgg aaagcggcaa atggattaag tgaggtgtac agctggctgg
     8641 atgaaggcag agatcagaat gcttggattg acttggaaat ccgtgtcgcg gaccagctgt
     8701 cacttgaaga gattcacagg ctgcggaagg cg
//