From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: D.pseudoobscura Adh gene for alcohol dehydrogenase
Date: 28 Dec 92 21:27:12 GMT
Distribution: bionet
Checksum: 55003 8

LOCUS       DROADHGN41   3483 bp ds-DNA             INV       01-DEC-1992
DEFINITION  D.pseudoobscura Adh gene for alcohol dehydrogenase
ACCESSION   X62221
KEYWORDS    ADH gene; alcohol dehydrogenase.
SOURCE      Drosophila pseudoobscura DNA.
  ORGANISM  Drosophila pseudoobscura
            Eukaryota; Animalia; Metazoa; Arthropoda; Uniramia; Insecta;
            Pterygota; Neoptera; Holometabola; Diptera; Brachycera;
            Cyclorrhapha; Schizophora; Drosophiloidea; Drosophilidae.
REFERENCE   1  (bases 1 to 3483)
  AUTHORS   Schaeffer,S.W. and Miller,E.L.
  TITLE     Estimates of gene flow in Drosophila pseudoobscura determined from
            nucleotide sequence analysis of the alcohol dehydrogenase region
  JOURNAL   Genetics 132, 471-480 (1992)
  STANDARD  full automatic
COMMENT     *source: strain=MV27; *source: isolate=Mesa Verde, Colorado;
            *source: chromosome=IV section 88;
               From EMBL    entry DPADHGN41;  dated 16-OCT-1992.
FEATURES             Location/Qualifiers
     mRNA            join(40..119,915..1020,1101..1505,1568..1983)
                     /note="adult mRNA (Adh)"
     mRNA            join(882..1020,1101..1505,1568..1983)
                     /note="larval mRNA (Adh)"
     promoter        8..14
                     /note="adult promoter (Adh)"
     prim_transcript 40..1983
                     /note="adult primary transcript (Adh)"
     exon            40..119
                     /note="adult exon 1a"
     intron          120..914
                     /note="adult intron"
     promoter        846..853
                     /note="larval promoter (Adh)"
     prim_transcript 882..1983
                     /note="larval primary transcript (Adh)"
     exon            882..1020
                     /note="larval exon 1"
     exon            915..1020
                     /note="adult exon 1b"
     intron          1021..1100
                     /number=1
     exon            1101..1505
                     /number=2
     intron          1506..1567
                     /number=2
     exon            1568..1983
                     /number=3
     polyA_signal    1960..1965
     polyA_site      1983
     CDS             join(928..1020,1101..1505,1568..1834)
                     /EC_number="1.1.1.1"
                     /gene="Adh"
                     /product="alcoholdehydrogenase"
                     /codon_start=1
                     /translation="MSLTNKNVVFVAGLGGIGLDTSRELVKRNLKNLVILDRIDNPAA
                     IAELKAINPKVTITFYPYDVTVPVAETTKLLKTIFAQVKTIDVLINGAGILDDHQIER
                     TIAVNYTGLVNTTTAILDFWDKRKGGPGGIICNIGSVTGFNAIYQVPVYSGSKAAVVN
                     FTSSLAKLAPITGVTAYTVNPGITKTTLVHKFNSWLDVEPRVAEKLLEHPTQTSQQCA
                     ENFVKAIELNKNGAIWKLDLGTLEPITWTQHWDSGI"
BASE COUNT      973 a    792 c    802 g    916 t
ORIGIN      
        1 cctagtgtat ttaaggcact gcacatgtcg aggaccacga ttattgtcta cagtgcagtc
       61 agtagttgtc aggtgcagca ggcggaccag catttcctta aatctactca atcgatcaag
      121 taagtaactg taatccaagt acagtcggcc catcccactc ggttctgccg tagctcaaga
      181 aatttcttga tagcgcagtt ccagttgtaa aacgtcgttt aaaaaaacat ctcgttggag
      241 cttgggttga aaaacgttcg agttgggcgt agaaaagttc aaatataaaa tatcaaaaaa
      301 gagaaaatat cttcccaaag cagctgtcca ttttttgtta aagtgataga tcaaaaattc
      361 gtatgacgta tttcccaagt gctctttatg caaataattt cctcaattct aattctacaa
      421 gaagcacttt tggctcttgt ccagaaaaaa caatttgttt acacttttcg gttgataaca
      481 gaggcggacc gacggctaag taacataaca gtcgcctgtt aagtaatgcc ctcactcgct
      541 gttagatggc cgcgcgtgtc gactccgata gaccagcaga cacgcagtag ttttcctcaa
      601 gaacctgaac tctaaacata gacataattt acgatacctt gagactcaaa taaaaataca
      661 agtatgagtg gtattctatt cctgattaga ctcgcacaca cacacgtacg agtattaaca
      721 taaatttgcc ggcaaataac caaaacaaaa cgaacgcaaa cgatccgaac acgtaatgcg
      781 agagataaga aacagaaaag ctcgacgtga aagaagcttc tggcggaagc tcttgccgaa
      841 ctccctataa atacgagaca gaaaaccaga ataaatataa taacaaactg ttgaattgtc
      901 ccatcgattc ccagatcaaa agacagaatg tctctcacca acaagaacgt cgttttcgtg
      961 gccggtctgg gcggcattgg cctggacacc agccgggagt tggtcaagcg taatctgaag
     1021 gtaagagaga tggagagaga gagcaatccc ttttccatgg aatctatgga atcctaaatt
     1081 taaaaattta tattttttag aacctggtca tcctggatcg cattgacaat ccggctgcca
     1141 ttgccgagct gaaggcaatc aatcccaagg tgacaatcac cttctatccc tacgatgtga
     1201 ctgtgcccgt cgctgagacc accaagctcc tgaagaccat ctttgcccag gtgaagacaa
     1261 tcgatgtcct gatcaacggt gctggcatcc tggacgatca tcagatcgag cgcaccattg
     1321 ccgttaacta cacgggcctg gtcaacacca ccacagccat tctggacttc tgggacaagc
     1381 gcaagggcgg cccaggcggc atcatttgca acattggctc cgtcaccggt ttcaatgcca
     1441 tctaccaggt gcccgtttac tccggctcca aggcggcggt ggttaacttt acctcctccc
     1501 tggcggtaag aaatcctaca actatcccaa aatccccaaa actaaatatt tctttgaaat
     1561 tccatagaaa ctggctccca ttactggtgt cactgcttac actgtcaatc ctggcatcac
     1621 taagaccact ctggtccaca aattcaactc gtggctggat gtggagcccc gtgtggcgga
     1681 gaagctgctc gagcatccca cccagacctc tcagcagtgc gccgagaact ttgtgaaggc
     1741 cattgagctg aacaagaacg gtgccatctg gaagttggat ttgggcacct tggagcccat
     1801 cacatggacc cagcactggg actcgggcat ctaaaccatc ccagagactc tatgggacat
     1861 ggcgttttag cttttagttt ctttttttcc actcaattgt tacgtatata catacacata
     1921 tggcaataag gctgatttga accccgtttt gaatatgata atacaaaatt atatttgaga
     1981 aatttcaaca aaatcgataa gagctatagc tctcttttaa ttaaaaaaaa ctagataaaa
     2041 agagcaatgg tcgtcagtgg tggtcagtgg tcgttgtggc ctctatcgat ttccacacaa
     2101 aaactttact gtttagtaat agaaaagaac ttgagaaagg cagccaaaag aatgtacgat
     2161 ctgacgggta agcatgtctg ctatgtggct gactgcggtg gcattgcact ggagaccagc
     2221 aaggttctca tgaccaagaa tatagcggtg agtggtagag tggtgtagag agtggagagt
     2281 ggttctggtt cttaaacaag tctctagtct ctagtctctt ggctgctgct ggtagctgtc
     2341 gaaactaatt aagttaatga ctttttcatg ttttgttttt gtccgctgac ttttgctgtt
     2401 caaagctaca attaagttta tgttttgagt ttgatctact tttgcttaag ggaagtactt
     2461 tcgattacgg attgctggca tataaaaata gataccaagg aatgtgcatc atattgattc
     2521 tatcttgatt ctttgcccag aaactggcca tccttcagag cgtggaaaac cccccggcca
     2581 tcgctcagtt acaatcgatt aagcacagca cacagatctt cttctggacc ttcgatgtga
     2641 ccatggcccg cgaggagatg aagaagtact tcgatgaggt catggtccag atggactaca
     2701 tcgatgtgct gatcaatggg gccaccctgt gcgatgagcg gaacattgat gccaccatca
     2761 atacgaatct gaccggcatg atgaacaccg tggccactgt gctgccctat atggaccgaa
     2821 agatgggcgg ttcgggtgga ctgattgtga atgtcacctc cgtcatagga ctggatccat
     2881 cgccggtctt ttgtgcgtac agtgcctcaa agtttggtgt gattgggttc acccgaagtc
     2941 tagcggtgag tgggcgagat cttagcttga aaatgctctc taatgtcttt cattctttcg
     3001 caggatcccc tgtatttcac ccaaaatggt gtggctgtca tggccgtctg ctgtggtccc
     3061 accaaagtgt ttgtcgatcg ggaactgact gccttcctgg catacggcca gtcctttgcc
     3121 gatcgcctgc gtcgtgcccc ctgccaatcg acggccgtct gcggccagaa tatagtaaat
     3181 gccatcgaga gatcggagaa cggacagatc tggattgccg acaagggcgg cctggagtcg
     3241 gtggccctcc actggtattg gcatatggcc gatcagtttg tgaactatat gcagagcact
     3301 gacgatgaag atcaggagtt ttttttgggc cagcgatgag gcatgccttg atccacggaa
     3361 taaaacatca aatgtgtcac aaaaagtagg caacttgttt gtgtgtttgg aaatagattt
     3421 gaaatatttt gaggtcatat gaataatgac ataaatgagg gtgcctgaaa gtatgccaag
     3481 ttt
//