From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: M.musculus gene for mitochondrial creatine kinase
Date: 28 Jul 92 10:05:58 GMT
Distribution: bionet
Checksum: 34062    12

LOCUS       MUSCRKING    6031 bp ds-DNA             ROD       28-JUL-1992
DEFINITION  M.musculus gene for mitochondrial creatine kinase
ACCESSION   Z13969
KEYWORDS    creatine kinase.
SOURCE      Mus musculus DNA.
  ORGANISM  Mus musculus
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Rodentia; Myomorpha; Muridae; Murinae.
REFERENCE   1  (bases 1 to 6031)
  AUTHORS   Wieringa,B.
  JOURNAL   Unpublished (1992)
  STANDARD  full staff_review
REFERENCE   2  (bases 1 to 6031)
  AUTHORS   Steeghs,K., Peters,W. and Wieringa,B.
  TITLE     Structure of the murine mitochondrial creatine kinase ubiquitous
            gene.
  JOURNAL   Nucleic Acids Res. Spec. Publ. (1992) In press
  STANDARD  full staff_review
COMMENT     *source: strain=F1 C57Bl6 x CBA; *source: tissue=Spleen;
            From EMBL    entry MMCRKING;  dated 30-JUN-1992.
FEATURES             Location/Qualifiers
     exon            <599..900
                     /partial
                     /note="5' boundary of this exon notknown."
                     /citation=[2]
                     /citation=[1]
     CDS             join(749..900,1485..1683,1851..1946,2442..2663,2788..2873,
                     2992..3115,4395..4529,4695..4820,5215..5331)
                     /function="Creatine phosphate energy shunt"
                     /EC_number="2.7.3.2"
                     /product="creatine kinaseisoenzyme, ubiquitous"
                     /codon_start=1
                     /translation="MAGPFSRLLSARPGLRLLALAGAGSLTAGILLRPESVGAAAAER
                     RRLYPPSAEYPDLRKHNNCMASHLTPAVYARLCDKTTPTGWTLDQCIQTGVDNPGHPF
                     IKTVGMVAGDEETYEVFAELFDPVIQERHNGYDPRTMKHTTDLDASKIRSGYFDERYV
                     LSSRVRTGRSIRGLSLPPACTRAERREVERVVVDALSGLKGDLAGRYYRLSEMTEAEQ
                     QQLIDDHFLFDKPVSPLLTAAGMARDWPDARGIWHNNEKSFLIWVNEEDHTRVISMEK
                     GGNMKRVFERFCRGLKEVEKLIQERGWEFMWNERLGYILTCPSNLGTGLRAGVHIKLP
                     LLSKDNRFPKILENLRLQKRGTGGVDTAATGSVFDISNLDRLGKSEVELVQLVIDGVN
                     YLIDCERRLERGQDIRIPPPLVHSKH"
     mat_peptide     join(863..900,1485..1683,1851..1946,2442..2663,2788..2873,
                     2992..3115,4395..4529,4695..4820,5215..5328)
                     /codon_start=1
     mRNA            join(<599..900,1485..1683,1851..1946,2442..2663,
                     2788..2873,2992..3115,4395..4529,4695..4820,5215..5453)
                     /partial
     5'UTR           599..748
                     /partial
                     /citation=[2]
                     /citation=[1]
     sig_peptide     749..862
     intron          901..1484
                     /number=1
     exon            1485..1683
                     /number=2
     intron          1684..1850
                     /number=21
     exon            1851..1946
                     /number=3
     intron          1947..2441
                     /number=31
     exon            2442..2663
                     /number=4
     intron          2664..2787
                     /number=41
     exon            2788..2873
                     /number=5
     intron          2874..2991
                     /number=51
     exon            2992..3115
                     /number=6
     intron          3116..4394
                     /number=61
     exon            4395..4529
                     /number=7
     intron          4530..4694
                     /number=71
     exon            4695..4820
                     /number=8
     intron          4821..5214
                     /number=81
     exon            5215..5453
                     /number=9
     3'UTR           5329..5453
     polyA_signal    5430..5435
     polyA_site      5454
BASE COUNT     1551 a   1390 c   1507 g   1581 t      2 others
ORIGIN
        1 ggatcctttc tcgaccttat taggaaagca taggtgtagc tgggagttcc ctggtgctct
       61 tcctggttct taggggaagg tgaccttgag gctgtgctgt ctttcccctg agctcgctca
      121 gtcttcctcc tctatctgtt tattctgtgg ttttcctgcg gctgactctc ctggggaaaa
      181 tggagagatg gcgtgcagat cgaattcaga aaagaggctg ggcaatgtca acctccccac
      241 ccccaccccc aaaacccaga aaatagacgg attgggtttc agatttaaac agagagggta
      301 tgaaagaagg ggggtttcag gttcgggaat ccccgtggtg ctgtcctcta cttagtgctg
      361 aaatctgacc accctcatcc ttggtgggcg tctgtctcca gcctcaggga gtaaatctcc
      421 agtcttctta attacctggc tagtgctacc caaccccacc cccgcacacc tagcgcggtg
      481 gcaggcggga aggcggagcc tgagtgagcc ccacccctgg agactgcggc tggggcctcc
      541 ctctgctctg gctggctcct tgcttgcgct tctcctgcag cctggtaggc accggctgcc
      601 acttcagctt cagccttcat cgtgacccct attttggctc cagtctccga cccagctccg
      661 tcctctctct acccggttgc atcttccctc ctagttcctg acttgcacca gatcctgctc
      721 tgaatctttg tagccgcatc cccaagccat ggctggtccc ttctcccgtc tgctgtctgc
      781 ccgccctgga ctcaggctcc tggctttggc tggagctggg tctctcaccg ccgggattct
      841 gctccgcccg gaatctgtag gagctgccgc tgctgaacgg aggagactgt atcccccgag
      901 gtattagagc ctgagatgca cgaggtgggg gctgggggtg gtagtggtgg tgatgggaac
      961 cgaacaggta gagaggataa cctgagcaaa agataggcct cgaagatgga tttcctgtac
     1021 agtttggaga tcctagccct gtaatggaag aacatgtcta aggacatgtc acccgatgtc
     1081 actgcctcca caccagcttt acctttgcaa attctaggtt cacccactgc ccaaatcctt
     1141 tttggatgac tagaagcttt tttttttttt tttttttttt aatctcgacc tattctcctt
     1201 gttctcccga attctcctga gtctctcccc tcccccatct ctaatgttat gctcactaga
     1261 gaaaagcaac tctgtagaca agggggaatt gaggtgttag aactattagg aaagaaaaga
     1321 ccctggaaat ccctgaagag attatcaaga tttagcctac ctctaattcc ccctccttcc
     1381 aataagcaaa gccagacatg gctcactgga cagctcccag ctgacatcag taggtctagg
     1441 ctcctgtgcc ctccctccat ggttactggg tacccccttc ccagcgctga gtacccagac
     1501 ctccgaaagc acaacaactg catggccagt cacctgaccc cagcagtcta tgcacggctc
     1561 tgcgacaaga ccacacccac tggttggaca ctagatcagt gcatccagac tggagtggac
     1621 aaccctggcc accccttcat caagactgtg ggcatggtgg ctggagatga ggagacctat
     1681 gaggtagggg gcccctcaga aatcccagtt ccttcctcct aaagatccct catgtctgtt
     1741 ccctcaagac tctggattgt ctatactcaa acgttctgat ccagtgatat caatctacaa
     1801 ctcaagtgtt tcttctttat aatcgcctct tcctcttaac tccttttcag gtatttgctg
     1861 aactgtttga ccctgtgatc caagagcggc ataatggata tgaccccaga acaatgaagc
     1921 acaccactga ccttgatgcc agtaaagtga gccaaatgtg acacttccga tatgtgcagc
     1981 cttgtgcaca atgttctgta tctccaatca ttacaccttg tttcctgact cagggttaga
     2041 ctgctgagct ttgtatattg ataagagaaa gaattgcata tcttgaagta tagcagatag
     2101 gaggacgtag tggataagag tctggataat ggagttgagg agaatctgat caaagtacaa
     2161 aatggtgaaa gaatttttaa gaagtgacta tgtcctggct gggtgtggtg gctcacacct
     2221 gtaatctcag ctctcagacg gctcagaaga gcactcctcc ctccaccaaa aaaaaaaaaa
     2281 aaaaggctgt acccaaagtg agaaatgtgg agaaatgacc acagtgtttt cagttagcat
     2341 gcctgtccaa tgtgtcttcc ttctggcacc tgctttataa tccccaagag ttcccacctg
     2401 ctgtgtttct atgatttata gtcatttctc ttattcctca gattcgttct ggctactttg
     2461 atgagaggta tgtattgtct tcaagagtca gaactggccg aagtatcagg ggactcagtc
     2521 tccctccagc ctgcactcgg gcagagcgaa gagaggtaga acgtgttgtg gtggatgctc
     2581 tgagtggcct gaagggtgac ctggctggac ggtactatag gctcagtgag atgacggagg
     2641 ccgaacagca gcagcttatt gatgtgaggg ccttgacagg gaacgggatt gtggaaaaag
     2701 aggtggaggg gagagtggac cagaaaaggt agatggcctg gatggtactg tgagaattct
     2761 tagacccaat ccctttattc tcctcaggac cattttctgt ttgataaacc tgtgtcccca
     2821 ttgctgactg cagcaggaat ggctcgagac tggcctgatg ctcgagggat ctggtatgga
     2881 gccaacacac ttttttggtt ttttttttcc cccttgtatg tgcaaagttt tttctccatc
     2941 taccccagtg cttgtcttgc ccactgatgg ctatacctat cctgccctca ggcacaacaa
     3001 tgagaagagt ttcttgatct gggtgaatga ggaggaccac acacgggtca tctctatgga
     3061 gaaaggcggc aacatgaaga gagtgtttga aagattctgc cggggcctca aagaggttgg
     3121 agaagggtat accagggagc taggggagag gaaatgagaa ataccacaca gcagcatagc
     3181 tagatcggct atatcacttg tcagtcaacc cagagtattt ctgatagtag aaagggtcat
     3241 tatcatgcta ggataatagt tacaaaccag ggccgtcctt ggnaaaccag ggcatatagt
     3301 tactctaaaa atagaaagag tgtcagtaac ctaaggaagg aaataaagca taatctttgc
     3361 caagtaatga aaagaaggaa ccaatgatca gcaagccagt gccggggtag gtggtatgag
     3421 gtagtatgtg cagttaagga gaacgtttga gcctggactg atggaaggcg tgagagattg
     3481 gtttgttcat gatacatcac caagttctga ncaaggctca ccttggctct ggagatctga
     3541 tccttgatgt attttcttgt atctacagtt ttatttttgg acttgtaaag aaagggtgct
     3601 tctcttttag attctagcca ggaagctcac actattttcg gcaaggaatg gataatgttc
     3661 tatgagtagc atagagaaat actctttaat tatatgactc tgtttatttg tctataaagt
     3721 tgaggactaa atgaaataat gtaggcagag gaattgttct ataataagtg ttagctttac
     3781 gatgtaaaaa tctccatgct tgtgattcct tctgagttac agagcatcat ctttaggtag
     3841 tgtggtcttt gtgtctattg ctctggcagg cccctgtctc ttccatccta atagtcatta
     3901 tggtggtcag gaaagcaaga acatctcttg aagtccccct cttctcacat gtacctgtga
     3961 atctggcttc tctgctgtac cttctcatgt ctccctgctc tattatccat gttcctttta
     4021 tagctaagag taggctctcc tgccttagca cctcgtttct gtccaaaggg accctttgtc
     4081 aaagatgtaa ttgatgcatg agaactccct gtcctgttta ctacttctat tcatttccca
     4141 agtcactctt aatgtatcat tctccaaagc ttcttctacg aacaatctat ctgccacaat
     4201 cttatcagca tgcatataat gcaacatcca agtgtccttc catgtgcaat gacaataagt
     4261 tttaacaaca acaaagagca tgtcattcat cgatcgtcaa gtttctcgtt gtagacatag
     4321 ttctagaatg tctgtggggc taagagggat ccctccctcc ctgatttttc acaacagaac
     4381 ctctgttgac acaggtggag aagctgatcc aggaacgagg ctgggagttc atgtggaatg
     4441 agcgtttagg ctacatcttg acctgtccat ctaacctggg cactggactt cgggcaggag
     4501 tccacatcaa actgccactg ctgagcaaag taaaggatat gtggggctag agggggtgta
     4561 agaaggggga cttggaaggg ggtggaggag acactctaaa aagagccagt cctaagggct
     4621 tatggctagt gggccaaggg taggagcagt ctcagaaact attgacatgc ccctgatctt
     4681 taccttgcat ctaggataac cgcttcccaa agatcctgga gaacctaaga ctgcaaaagc
     4741 gtggaactgg aggagtggac actgctgcta caggcagcgt ctttgacatc tctaatttgg
     4801 atcgacttgg caagtcagag gtgaggtctt atgaatcagg ttaagaggtt gagataaggc
     4861 agtgagtgag cctcaggaac ataatagaat ctgaagcaat ggtcaggttg agtggggtag
     4921 ggaagagaaa acgagttttc agagatggaa gagctaaaga aactcaggtc aggcaaggtg
     4981 gtctgtgctt ttaataccag cagaagcaag acagatctct gtgagtctca ggccagccag
     5041 ggctacataa tgagatcctg tcacaaaaac tgaaaaaaga aaaagaagga aaagaaggaa
     5101 ggagggaagg aaggaaggga gagagagaga gagggaggga gagagaggaa gagagagaga
     5161 ttaagagaag ctcgggtaca ccgggaagca gggatcaagg actgtgttct tcaggtggag
     5221 ctggtgcagc tcgtcatcga tggggtgaac tatttgattg actgtgaacg gcgtctggag
     5281 agaggacagg atattcgaat ccctccacct cttgtccaca gcaaacatta actccccatc
     5341 cccagcggat gagtcaagac ccccaggact tctgcatatt taacagtggc ccagtctact
     5401 tgccctggac ctgcctctct ccctgtccta gtaaagactc cacattatgt tgcagctgtc
     5461 tgtgttattt ctattggtgt ggtgaggagg aagtaggaaa tgaaaagaaa cagtgaggtt
     5521 attcatgatg gcaagaggcg tatatgccag gagcacagtt ctcaggtggg tggaatgcat
     5581 tggaatttta cccacaatcc tcatccactt actgagaata attagtatgc acttccattt
     5641 acaagttgtt cagtatgatg taaattttct ccctcaactg aaggaacagt tatcataata
     5701 ggtggccaaa aaaaatgctg acggggcaat gccagggccc aggaggcttg gaaccagtcg
     5761 tagagacccc aggctgagtt tcgacctaga atgagacaag gaatgggagg aaaaaaaatc
     5821 acagttccgt tccccaggag cctttgatgt ttcaaattta gctctgtgta tagatccctc
     5881 tactccagag attctcctgt gtggggtctg aggaccagac agcaccaagt taggacttag
     5941 atggactctg ggtcagatga cagcttatca aactatgcag ttggtaacgt acccagctgc
     6001 tctgggatct ccttcccttc gtgttgggcc c
//