From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Escherichia coli hsdh gene encoding 7alpha-hydroxysteroid
Date: 21 Jul 92 10:04:17 GMT
Article-I.D.: genbank.Jul.21.03.04.15.1992.18026
Distribution: bionet
Checksum: 62622     5

LOCUS       ECOHSDH      1785 bp ds-DNA             BCT       21-JUL-1992
DEFINITION  Escherichia coli hsdh gene encoding 7alpha-hydroxysteroid
            dehydrogenase.
ACCESSION   D10497 D90475
KEYWORDS    dehydrogenase; hydroxysteroid dehydrogenase.
SOURCE      Escherichia coli (strain HB101) DNA.
  ORGANISM  Escherichia coli
            Prokaryotae; Gram-negative facultatively anaerobic rods;
            Enterobacteriaceae.
REFERENCE   1  (sites)
  AUTHORS   Macdonald,I.A., Williams,C.N. and Mahony,D.E.
  TITLE     7alpha-hydroxysteroid dehydrogenase from escherichia coli B:
            preliminary studies
  JOURNAL   Biochim. Biophys. Acta 309, 243-253 (1973)
  STANDARD  full staff_review
REFERENCE   2  (bases 1 to 1785)
  AUTHORS   Yoshimoto,T., Higashi,H., Kanatani,A., Lin,X.S., Nagai,H.,
            Oyama,H., Kurazono,K. and Tsuru,D.
  TITLE     Cloning and sequencing of the 7alpha-hydroxysteroid dehydrogenase
            gene from Escherichia coli HB101 and characterization of the
            expressed enzyme
  JOURNAL   J. Bacteriol. 173, 2173-2179 (1991)
  STANDARD  full staff_review
COMMENT     Data kindly submitted in computer readable form by:
            Tadashi Yoshimoto
            School of Pharmaceutical Science
            Nagasaki University
            1-14 Bunkyo-machi
            Nagasaki 852
            Japan
            Phone:  0958-47-1111 x 2551
            Fax:    0958-44-6774
            [1]  sites.
FEATURES             Location/Qualifiers
     -35_signal      699..704
     -10_signal      720..726
     RBS             739..744
     CDS             750..1517
                     /EC_number="1.1.1.159"
                     /product="7alpha-hydroxysteroid dehydrogenase"
                     /gene="hsdh"
                     /codon_start=1
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLGGQAFACRCDITSEQELSALADFAISKLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTDALKSVITPEIEQKMLQHTPIRRL
                     GQPQDIANAALFLCSPAASWVSGQILTVSGGGVQELN"
     repeat_unit     1530..1541
                     /function="transcription terminator"
                     /rpt_type=inverted
     repeat_unit     1549..1560
                     /function="transcription terminator"
                     /rpt_type=inverted
BASE COUNT      475 a    413 c    446 g    451 t
ORIGIN      5 bp upstream of PstI site.
        1 ctgcaggaag tagcgatgac ctgcgacgga tggcagaaga aaaagctatc ccggtgattt
       61 tcgcttcccg tgccagttat cttgatgatg ttgatacggt tcgcccggac aacatgcagg
      121 ctgcacagtt gttgacggag catctcattc gcaatgggca tcagcggatc ggctggctgg
      181 gagggcaaag ttcctcatta acccgtgcag aacggttggg ggctattgtg caactctact
      241 aaaatttggc ctgcgtttca tagcgattgg gtgttggagt gcacttcagc agaagcaagc
      301 cgcggaacga tcacggcgct tttacgtcat aacccgacca tcagtgccgt ggtttgctat
      361 aacgaaacta ttgcgatggg ggcatggttt ggtttgctca aagcagggcc gcaaagcggg
      421 gaaagcggag tcgatcgtta ctttgagcaa caggtttcgc tggcggcatt taccgatgcg
      481 acaccaacca cacttgatga tatacctgtt acctgggcca gcacgccagc gcggaacttg
      541 gtatcacact tgcggatcgc atgatgcaaa aaatcaccca tgaagagacg cattcacgca
      601 atcttattat tcccgcccgg ctcattgcag cgaaataatc ctctctttat ctgctatacc
      661 tggtagtgtc ctttcctcaa ggttaatgat aaaaaatgtt gcacacaggc ggtgcctttt
      721 atttttactg aagttcacag gaggtttatg tgtttaattc tgacaacctg agactcgacg
      781 gaaaatgcgc catcatcaca ggtgcgggtg caggtattgg taaagaaatc gccattacat
      841 tcgcgacagc tggcgcatct gtggtggtca gtgatattaa cgccgacgca gctaaccatg
      901 ttgtagacga aattcaacaa ctgggtggtc aggcatttgc ctgccgttgt gatattactt
      961 ccgaacagga actctctgca ctggcagact ttgctatcag taagctgggt aaagttgata
     1021 ttctggttaa caacgccggt ggcggtggac ctaaaccgtt tgatatgcca atggcggatt
     1081 ttcgccgtgc ttatgaactg aatgtgtttt cttttttcca tctgtcacaa cttgttgcgc
     1141 cagaaatgga aaaaaatggc ggtggcgtta ttctgaccat cacttctatg gcggcagaaa
     1201 ataaaaatat aaacatgact tcctatgcat catctaaagc tgcggccagt catctggtca
     1261 gaaatatggc gtttgacctg ggtgaaaaaa atattcgggt aaatggcatt gcgccggggg
     1321 caatattaac cgatgccctg aaatccgtta ttacaccaga aattgaacaa aaaatgttac
     1381 agcacacgcc gatcagacgt ctgggccaac cgcaagatat tgctaacgca gcgctgttcc
     1441 tttgctcgcc tgctgcgagc tgggtaagcg gacaaattct caccgtctcc ggtggtgggg
     1501 tacaggagct caattaatac actaacggac cggtaaacaa ccgtgcgtgt tgtttaccgg
     1561 gataaactca tcaacgtctc tgctaaataa ctggcagcca aatcacggct attggttaac
     1621 caatttcaga gtgaaaagta tacgaataga gttggccttc gcactattca acagcaatga
     1681 taggcgctca cctgacaacg cggtaaacta gttattcacg ctaactataa tggtttaatg
     1741 atggataaca tgcagactga agcacaaccg acacggcacg gatcc
//