From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: tetM=tetracycline resistance determinant [Enterococcus faecalis,
Date: 6 Jan 93 07:05:00 GMT
Distribution: bionet
Checksum: 49863 7

LOCUS       S111076      2790 bp    DNA             BCT       02-DEC-1992
DEFINITION  tetM=tetracycline resistance determinant [Enterococcus faecalis,
            conjugative transposon Tn916, Transposon, 2790 nt]
ACCESSION   S42413 M85225
KEYWORDS    .
SOURCE      Enterococcus faecalis conjugative transposon Tn916
  ORGANISM  Enterococcus faecalis
            Unclassified.
REFERENCE   1  (bases 1 to 2790)
  AUTHORS   Su,Y.A., He,P. and Clewell,D.B.
  TITLE     Characterization of the tet(M) determinant of Tn916: evidence for
            regulation by transcription attenuation.
  JOURNAL   Antimicrob. Agents Chemother. 36, 769-778 (1992)
  STANDARD  full automatic
COMMENT     This entry [NCBI gibbsq 111076] was created by the journal scanning
            component of NCBI/GenBank at the National Library of Medicine.
            This sequence comes from Fig. 2.
FEATURES             Location/Qualifiers
     mRNA            248..2761
     CDS             520..2439
                     /note="tetracycline resistance determinant;  For the
                     protein sequence (NCBI gibbsq 111078):  Method: conceptual
                     translation supplied by author.  This sequence comes from
                     Fig. 2."
                     /gene="tetM"
                     /product="TetM"
                     /codon_start=1
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNVCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     CDS             418..504
                     /note="Description: leader peptide 5' of TetM;  For the
                     protein sequence (NCBI gibbsq 111079):  Method: conceptual
                     translation supplied by author.  This sequence comes from
                     Fig. 2."
                     /codon_start=1
                     /translation="MLCMPMVMHKNPSDKSIYHWDFYALLGF"
BASE COUNT      965 a    416 c    553 g    856 t
ORIGIN      
        1 aatcctattc acaatcgtaa ggataatcaa gtcacggtat cgctgacagt ggagtatatc
       61 gaccagcaga ccaaagcaac gcaggtatct caatttgatt tggtacttga aaagaacggg
      121 agtaattgga agatatagaa taacaaatat tggtacatta ttacagctat tttgtaatca
      181 cgtactctct ttgataaaaa attggagatt cctttacaaa tatgctctta cgtgctatta
      241 tttaagtatc tatttaaaag gagttaataa atatgcggca aggtattatt aaataaactg
      301 tcaatttgat agcgggaaca aataattgga tgtccttttt taggagggct tagttttttg
      361 tacccagttt aagaatacct ttatcatgtg attctaaagt atccggagaa tatctgtatg
      421 ctttgtatgc ctatggttat gcataaaaat cccagtgata agagtattta tcactgggat
      481 ttttatgccc ttttgggctt ttgaatggag gaaaatcaca tgaaaattat taatattgga
      541 gttttagctc atgttgatgc aggaaaaact accttaacag aaagcttatt atataacagt
      601 ggagcgatta cagaattagg aagcgtggac aaaggtacaa cgaggacgga taatacgctt
      661 ttagaacgtc agagaggaat tacaattcag acaggaataa cctcttttca gtgggaaaat
      721 acgaaggtga acatcataga cacgccagga catatggatt tcttagcaga agtatatcgt
      781 tcattatcag ttttagatgg ggcaattcta ctgatttctg caaaagatgg cgtacaagca
      841 caaactcgta tattatttca tgcacttagg aaaatgggga ttcccacaat cttttttatc
      901 aataagattg accaaaatgg aattgattta tcaacggttt atcaggatat taaagagaaa
      961 ctttctgccg aaattgtaat caaacagaag gtagaactgt atcctaatgt gtgtgtgacg
     1021 aactttaccg aatctgaaca atgggatacg gtaatagagg gaaacgatga ccttttagag
     1081 aaatatatgt ccggtaaatc attagaagca ttggaactcg aacaagagga aagcataaga
     1141 tttcagaatt gttctctgtt ccctctttat catggaagtg caaaaagtaa tatagggatt
     1201 gataacctta tagaagttat tactaataaa ttttattcat caacacatcg aggtccgtct
     1261 gaactttgcg gaaatgtttt caaaattgaa tatacaaaaa aaagacaacg tcttgcatat
     1321 atacgccttt atagtggagt actacattta cgagattcgg ttagagtatc agaaaaagaa
     1381 aaaataaaag ttacagaaat gtatacttca ataaatggtg aattatgtaa gattgataga
     1441 gcttattctg gagaaattgt tattttgcaa aatgagtttt tgaagttaaa tagtgttctt
     1501 ggagatacaa aactattgcc acagagaaaa aagattgaaa atccgcaccc tctactacaa
     1561 acaactgttg aaccgagtaa acctgaacag agagaaatgt tgcttgatgc ccttttggaa
     1621 atctcagata gtgatccgct tctacgatat tacgtggatt ctacgacaca tgaaattata
     1681 ctttctttct tagggaaagt acaaatggaa gtgattagtg cactgttgca agaaaagtat
     1741 catgtggaga tagaactaaa agagcctaca gtcatttata tggagagacc gttaaaaaat
     1801 gcagaatata ccattcacat cgaagtgccg ccaaatcctt tctgggcttc cattggttta
     1861 tctgtatcac cgcttccgtt gggaagtgga atgcagtatg agagctcggt ttctcttgga
     1921 tacttaaatc aatcatttca aaatgcagtt atggaaggga tacgctatgg ttgtgaacaa
     1981 ggattgtatg gttggaatgt gacggactgt aaaatctgtt ttaagtatgg cttatactat
     2041 agccctgtta gtaccccagc agattttcgg atgcttgctc ctattgtatt ggaacaagtc
     2101 ttaaaaaaag ctggaacaga attgttagag ccatatctta gttttaaaat ttatgcgcca
     2161 caggaatatc tttcacgagc atacaacgat gctcctaaat attgtgcgaa catcgtagac
     2221 actcaattga aaaataatga ggtcattctt agtggagaaa tccctgctcg gtgtattcaa
     2281 gaatatcgta gtgatttaac tttctttaca aatggacgta gtgtttgttt aacagagtta
     2341 aaagggtacc atgttactac cggtgaacct gtttgccagc cccgtcgtcc aaatagtcgg
     2401 atagataaag tacgatatat gttcaataaa ataacttagt gtattttatg ttgttatata
     2461 aatatggttt cttgttaaat aagatgaaat attttttaat aaagatttga attaaagtgt
     2521 aaaggaggag atagttatta taaactacaa gtggatattg tgtcctgtat gtggaaataa
     2581 aacacgatta aagataaggg aagatactga attaaaaaaa ttccccctct attgtccgaa
     2641 atgcagacaa gaaaatttaa ttgaaataaa gcagttcaaa gtaactgtga ttacagagcc
     2701 agacgcaaag acgcagagcc gataaaatga gattaataca atctcatttt atcggctctt
     2761 tccgttatgt atggattctt ttaattagtc
//