From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: LEU2=3-isopropylmalate dehydrogenase [Candida boidinii, Genomic,
Date: 3 Jan 93 22:19:46 GMT
Distribution: bionet
Checksum: 32259 5

LOCUS       S113056      1700 bp    DNA             UNA       24-NOV-1992
DEFINITION  LEU2=3-isopropylmalate dehydrogenase [Candida boidinii, Genomic,
            1700 nt]
ACCESSION   S44582 M98832
KEYWORDS    .
SOURCE      Candida boidinii
  ORGANISM  Candida boidinii
            Unclassified.
REFERENCE   1  (bases 1 to 1700)
  AUTHORS   Sakai,Y. and Tani,Y.
  TITLE     Directed mutagenesis in an asporogenous methylotrophic yeast:
            cloning, sequencing, and one-step gene disruption of the
            3-isopropylmalate dehydrogenase gene (LEU2) of Candida boidinii to
            derive doubly auxotrophic marker strains.
  JOURNAL   J. Bacteriol. 174, 5988-5993 (1992)
  STANDARD  full automatic
COMMENT     This entry [NCBI gibbsq 113056] was created by the journal scanning
            component of NCBI/GenBank at the National Library of Medicine.
            This sequence comes from Fig. 1.  The authors begin their numbering
            at -320.  Zero is not present in the numbering.
FEATURES             Location/Qualifiers
     CDS             321..1418
                     /note="For the protein sequence (NCBI gibbsq 113057):
                     This sequence comes from Fig. 1."
                     /gene="LEU2"
                     /product="3-isopropylmalate dehydrogenase"
                     /codon_start=1
                     /translation="MSIIEKKIVLLPGDHVGVEVVEEAVKILKSISEVKPEIQFKFEN
                     HLIGGAAIDATGVPLPDEALEAAKKSDAVLLGAVGGPKWGTGEVRPEQGLLKIRKELN
                     LYANLRPCNFASDKLLDLSPLKSDIVKGTDFTVVRELVGGIYFGDRVEDDGSGFASDS
                     ESYSVPEVERITRMAAFLSLQNDPPLPIWSLDKANVLASSRLWRKTVDRVIKEEFPKL
                     TVQHQLIDSAAMILVKSPTKLNGIVITNNMFGDIISDEASVIPGSLGLLPSASLASLP
                     DTNQAFGLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLVKEGNAVEEAVRKVL
                     DQGIMTGDLGGNNSTTEVGDAIAKEVKLLLA"
BASE COUNT      614 a    170 c    294 g    622 t
ORIGIN      
        1 agaatgagaa aagttgccgg taccggaacc atatttcttg attacataag ccatagtaat
       61 attatattag tacaatataa gttattatta acacaatcaa ttattcaatt ttcaaaatta
      121 attgagaaaa ttgggaatta gttatatgac tgactaactt attcaacttg tggagaaaat
      181 agagtatgaa agttgttcat atatataagt taattattca tttttttaat ttaaataatt
      241 aagtatttaa tatatttaag tatatatata tatatatata tatttgagta tagtatagta
      301 tagtatataa gtatattaaa atgagtataa ttgaaaaaaa aattgtttta ttaccaggtg
      361 atcatgttgg tgttgaagtt gttgaagaag cagttaaaat tttaaaatca attagtgaag
      421 ttaaaccaga gattcaattt aaatttgaga atcatttaat tggtggtgca gcaattgatg
      481 ctacaggtgt tccattacct gatgaagcat tagaagcagc taaaaaatca gatgctgtat
      541 tattaggagc tgttggtgga cctaaatggg gtactggtga agttagacca gagcaaggtt
      601 tattaaagat tagaaaagaa ttaaatttat atgctaattt aaggccatgt aattttgcca
      661 gtgataaatt attagattta tcaccattaa aatctgatat tgttaaaggt acagatttta
      721 cagttgttag agaattagtt ggtggtattt attttggaga tagggttgaa gatgatggta
      781 gtggatttgc cagtgatagt gaaagttatt cagttccaga agttgaaagg atcactagaa
      841 tggcagcatt tttatcatta caaaatgatc caccattacc aatttggtca ttagataaag
      901 ccaatgtttt agcatctagt agattatgga ggaaaacagt tgatagagtt attaaagaag
      961 aatttccaaa attaacagtt caacatcaat taattgattc agcagcaatg atattagtta
     1021 aatcaccaac taaattaaat ggtattgtta ttacaaataa tatgtttgga gatattattt
     1081 ctgatgaagc atcagttatt ccaggatcat taggtttatt accatcagca tcattggcat
     1141 cattaccaga taccaatcaa gcatttggat tatatgagcc atgtcatgga tcagcacctg
     1201 atttaccaaa gaataaagtc aatccaattg caactatatt atcagcggca atgatgctta
     1261 aattatcatt aaatttagtt aaagaaggta atgcagttga agaagctgtt agaaaagtat
     1321 tagatcaagg tattatgact ggagatcttg gtggtaataa ttcaactact gaagttggtg
     1381 atgctattgc taaagaagtt aaattacttt tagcttaatt aatttaattt aattagatga
     1441 atttatataa gaattaaatt aaatttaata aaaataataa gaataatgaa tatacagtat
     1501 atatatatat atgaatacgt aataacatgt aataattgat aaatttgagg atagagtcta
     1561 gatcaattta ataatggtta caataatagt atatttggct ctgatcaggc gttgttcttt
     1621 ctttgtttct ttgtttcttt gtttctttgt ttctttgttt ctttgtttct ttgtttcttt
     1681 gtttgattgt ttgaaagctt
//