From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: S.cerevisiae SK01 gene for CRE-binding bZIP protein
Date: 28 Dec 92 21:47:21 GMT
Distribution: bionet
Checksum: 53599 6

LOCUS       YSCSKO1      3000 bp ds-DNA             PLN       02-DEC-1992
DEFINITION  S.cerevisiae SK01 gene for CRE-binding bZIP protein
ACCESSION   X67875
KEYWORDS    binding protein.
SOURCE      Saccharomyces cerevisiae DNA.
  ORGANISM  Saccharomyces cerevisiae
            Eukaryota; Fungi; Eumycota; Hemiascomycetes; Endomycetales;
            Saccharomycetaceae.
REFERENCE   1  (bases 1 to 3000)
  AUTHORS   Nehlin,J.O., Calberg,M. and Ronne,H.
  TITLE     Yeast SKO1 gene encodes a bZIP protein that binds to the CRE motif
            and acts as a repressor of transcription
  JOURNAL   Nucleic Acids Res. 20, 5271-5278 (1992)
  STANDARD  full automatic
COMMENT     *source: strain=W303-1A; *source: haplotype=haploid; *source:
            cell_line=H100; *source: clone_library=genomic Yeast in pHR81;
            *source: chromosome=XIV L; *source: map_position=31 cM telomeric TO
            met4 centimorgan;
               From EMBL    entry SCSKO1;  dated 14-OCT-1992.
FEATURES             Location/Qualifiers
     misc_feature    327..334
                     /note="heat shock element"
     misc_signal     420..427
                     /note="cell cycle box"
     CDS             841..2784
                     /gene="SKO1"
                     /product="cre-binding bZIP protein"
                     /codon_start=1
                     /translation="MSSEERSRQPSTVSTFDLEPNPFEQSFASSKKALSLPGTISHPS
                     LPKELSRNNSTSTITQHSQRSTHSLNSIPEENGNSTVTDNSNHNDVKKDSPSFLPGQQ
                     RPTIISPPILTPGGSKRLPPLLLSPSILYQANSTTNPSQNSHSVSVSNSNPSAIGVSS
                     TSGSLYPNSSSPSGTSLIRQPRNSNVTTSNSGNGFPTNDSQMPGFLLNLSKSGLTPNE
                     SNIRTGLTPGILTQSYNYPVLPSINKNTITGSKNVNKSVTVNGSIENHPHVNIMHPTV
                     NGTPLTPGLSSLLNLPSTGVLANPVFKSTPTTNTTDGTVNNSISNSNFSPNTSTKAAV
                     KMDNPAEFNAIEHSAHNHKENENLTTQIENNDQFNNKTRKRKRRMSSTSSTSKASRKN
                     SISRKNSAVTTAPAQKDDVENNKISNNVTLDENEEQERKRKEFLERNRVAASKFRKRK
                     KEYIKKIENDLQFYESEYDDLTQVIGKLCGIIPSSSSNSQFNVNVSTPSSSSPPSTSL
                     IALLESSISRSDYSSAMSVLSNMKQLICETNFYRRGGKNPRDDMDGQEDSFNKDTNVV
                     KSENAGYPSVNSRPIILDKKYSLNSGANISKSNTTTNNVGNSAQNIINSCYSVTNPLV
                     INANSDTHDTNKHDVLSTLPHNN"
BASE COUNT     1048 a    619 c    469 g    864 t
ORIGIN      
        1 gttataaata tttataaatt aagacagata tataaagtta aaataaatac caaaagaaca
       61 tagacaatcg taagagaata agaatccttt atcctagagc catccatctg ttatttttga
      121 tgttgctgac tttttttctg ataaattcta ttattttaat agtaaagagg tgagtatttc
      181 gcctaaacgt aaacaaaacg ataataaaaa aaaaaaaaaa tgaagacaaa atgttcagat
      241 agtcaattat attgtaataa catgaaacgg aacaataaca gtacccagaa ttcaatttca
      301 atggcgttgt gtcttctttt ctctaagaac tttcataaac caagtcgtac ttaaccgcgc
      361 tagcgataac cctgctaact ttgtcgcaga gggaaaaaag tagaatcgaa gggaaaaatc
      421 acgaaaatta actgggctga atagaatgaa ttcagtcata cggtgatggt tttcgaaaca
      481 aactgcacat ttttccagtt aaaaggacca agtttggtta ctgatacgtg agactaattt
      541 gttctccaga aacacaataa tttggtgcta cattgccaac gctcaggagt ggttttaagc
      601 tcagaaaagt tactaccaaa ggggttcaac gtaaaagtga attgtgtttt cttatcgaga
      661 aaaactcttt tattattcaa gtgagttttc cacttattag attcctatct ttttaccgat
      721 ctctattatt tgataagttc cttaatcaat tcgtggattt ttatttttat ttcattcttt
      781 tcatttcaat ttctgcattc caaatacacc tgcccagtct ctagaccctg cttaatcatt
      841 atgtcaagcg aggaacgctc gagacaacca agtactgttt caactttcga tttagaaccc
      901 aatccttttg aacaaagctt cgcctcttcc aagaaggctt tgtcacttcc aggcacgatc
      961 tcccatccgt ctctaccaaa agagctttca cgaaacaatt ctacctcaac gataacacaa
     1021 cattcccaac gttccactca tagtttgaac agtattccag aagaaaatgg gaattcaacc
     1081 gttacagata atagtaatca taatgacgta aaaaaagact cacctagttt tttaccaggc
     1141 caacaaagac ctactataat atctccgcct attctcacac ctggtgggtc gaaaagatta
     1201 ccacctctac tcctttctcc ctctatttta tatcaggcaa attcaactac gaatcccagt
     1261 cagaattcac attctgtttc agtctctaat tcaaacccta gtgcaatagg cgtttcttct
     1321 acctccggat ctctgtatcc aaacagctct tctccttcag ggacttcact tatacgccag
     1381 ccacgaaact cgaacgtgac cacaagtaat tctggtaacg gcttccccac gaatgattca
     1441 caaatgcctg gctttttatt aaacttgtcc aaatctgggt tgacacctaa tgagtccaat
     1501 atcaggaccg ggttgacacc cggtattctt acgcaatctt acaattatcc cgtattgccg
     1561 tcaattaata aaaatactat aacaggtagc aaaaatgtca acaaaagtgt cacagtgaat
     1621 ggaagtattg aaaaccaccc tcatgttaat ataatgcacc caactgtaaa tggtacacca
     1681 cttacgccgg gattgagttc tctgctaaac ttaccatcta ctggagtttt ggctaatcca
     1741 gtattcaaat caacacctac aacaaatacc acagatggta ccgtcaacaa cagcatcagt
     1801 aattccaatt tttccccaaa tacttcaacg aaagcggctg tcaaaatgga taatccggca
     1861 gagttcaatg ccatcgagca ctccgctcat aatcacaagg agaatgaaaa tttaacgact
     1921 caaattgaga acaatgacca gttcaataac aaaacacgaa aaagaaagag aaggatgtct
     1981 agcacaagtt ctacttctaa ggcttcaaga aaaaattcca tatcaagaaa aaactcagca
     2041 gttacgactg caccagcaca aaaagatgat gttgaaaata ataaaatttc aaacaacgta
     2101 acacttgatg agaatgaaga gcaagaaaga aaaagaaagg agtttttaga aagaaataga
     2161 gtagctgcat ctaaatttag aaaaagaaag aaagagtaca tcaagaaaat tgaaaatgat
     2221 ctacaattct atgaatctga atatgacgat ctaactcagg tcattgggaa gctatgcggc
     2281 ataataccct caagctcctc gaattcccaa ttcaatgtga atgtttcaac tccgtcatca
     2341 tcatcaccac catctacatc tttaatagca ttgttagaga gtagcatttc aaggagtgat
     2401 tattcaagtg caatgtcagt attatcaaac atgaagcaat tgatatgtga aacgaatttt
     2461 taccgaaggg gaggcaaaaa cccaagggac gacatggatg gccaagaaga cagcttcaat
     2521 aaggacacta acgttgtcaa aagcgaaaat gcgggctatc cttccgttaa ttcaagacca
     2581 ataattctag ataaaaaata ctcactgaac tctggagcaa atatcagcaa aagtaacaca
     2641 actactaata atgtgggaaa tagtgcacag aatataatca attcatgcta ctctgttact
     2701 aatccattgg taataaatgc aaattccgat acccatgata ctaataagca tgatgtacta
     2761 tccactctac ctcacaataa ttgacgagat agcgacgggg ttcttaaata gtcttctatc
     2821 tgatgctttc gtactcttca acctgtccgc ttttctcatt gcaaatattc cactataaag
     2881 ttccttttaa gaggaaatcc cttttattct cccgcttttt tctgtattta tgtaacttca
     2941 agattttttt tctatatttg atataacttt aatagggttc ttcttttcag aagtttatca
//