From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Mouse perforin mRNA, complete cds.
Date: 6 Nov 92 18:31:37 GMT
Distribution: bionet
Checksum: 38648 5

LOCUS       MUSPERA      1985 bp ss-mRNA            ROD       02-NOV-1992
DEFINITION  Mouse perforin mRNA, complete cds.
ACCESSION   M23182
KEYWORDS    perforin; pore-forming protein.
SOURCE      Mouse cytolytic T-lymphocyte (cell ine CTLLA11), cDNA to mRNA,
            clones PFP6 and PFP15.
  ORGANISM  Mus musculus
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Rodentia; Myomorpha; Muridae; Murinae.
REFERENCE   1  (bases 1 to 1985)
  AUTHORS   Kwon,B.S., Wakulchik,M., Liu,C.-C., Persechini,P.M., Trapani,J.A.,
            Haq,A.K., Kim,Y. and Young,J.D.-E.
  TITLE     The structure of the mouse lymphocyte pore-forming protein perforin
  JOURNAL   Biochem. Biophys. Res. Commun. 158, 1-10 (1989)
  STANDARD  full automatic
COMMENT     Draft entry and clean copy of sequence for [1] kindly provided by
            B.S.Kwan, 17-MAR-1989.
FEATURES             Location/Qualifiers
     sig_peptide     195..254
                     /codon_start=1
     mat_peptide     255..1856
                     /product="perforin"
                     /codon_start=1
     CDS             195..1859
                     /product="perforin"
                     /codon_start=1
                     /translation="MATCLFLLGLFLLLPRPVPAPCYTATRSECKQKHKFVPGVWMAG
                     EGMDVTTLRRSGSFPVNTQRFLRPDRTCTLCKNSLMRDATQRLPVAITHWRPHSSHCQ
                     RNVAAAKVHSTEGVAREAAANINNDWRVGLDVNPRPEANMRASVAGSHSKVANFAAEK
                     TYQDQYNFNSDTVECRMYSFRLVQKPPLHLDFKKALRALPRNFNSSTEHAYHRLISSY
                     GTHFITAVDLAGRISVLTALRTCQLTLNGLTADEVGDCLNVEAQVSIGAQASVSSEYK
                     ACEEKKKQHKMATSFHQTYRERHVEVLGGPLDSTHDLLFGNQATPEQFSTWTASLPSN
                     PGLVDYSLEPLHTLLEEQNPKREALRQAISHYIMSRARWQNCSRPCRSGQHKSSHDSC
                     QCECQDSKVTNQDCCPRQRGLAHLVVSNFRAEHLWGDYTTATDAYLKVFFGGQEFRTG
                     VVWNNNNPRWTDKMDFENVLLSTGGPLRVQVWDADYGWDDDLLGSCDRSPHSGFHEVT
                     CELNHGRVKFSYHAKCLPHLTGGTCLEYAPQGLLGDPPGNRSGAVW"
BASE COUNT      453 a    569 c    540 g    423 t
ORIGIN      
        1 ttctgcagtt gtcacagatg gaggagatct ttttgtttgt ggcctgaata aagatgggca
       61 gttggggctt ggtcacacag aggaagttct gcgttttacc atctgcaagc ctctccgtgg
      121 ttgtcccatc cgacagtggc gtcttggtgg gacttcagct ttccagagtt tatgactact
      181 gtgcctgcag catcatggcc acgtgcctgt tcctcctggg ccttttcctg ctgctgccac
      241 gacctgtccc tgctccctgc tacactgcca ctcggtcaga atgcaagcag aagcacaagt
      301 tcgtgccagg tgtatggatg gctggggaag gcatggatgt gactaccctc cgccgctccg
      361 gctccttccc agtgaacaca cagaggttcc tgaggcctga ccgcacctgc accctctgta
      421 aaaactccct aatgagagac gccacacagc gcctacctgt ggcaatcacc cactggcggc
      481 ctcacagctc acactgccag cgtaatgtgg ccgcagccaa ggtccactcc acggagggtg
      541 tggcccggga ggcagctgct aatatcaata acgactggcg tgtggggctg gatgtgaacc
      601 ctaggccaga ggcaaacatg cgcgcctccg tggctggctc ccactccaag gtagccaatt
      661 ttgcagctga gaagacctat caggaccagt acaactttaa tagcgacaca gtagagtgtc
      721 gcatgtacag ttttcgcctg gtacaaaaac ctccactcca ccttgacttc aaaaaggcgc
      781 tcagagccct cccccgcaac tttaacagct ccacagagca tgcttaccac aggctcatct
      841 cctcctatgg cacgcacttt atcacggctg tggacctcgc tggccgcatc tcggtcctta
      901 cagccctgcg tacctgtcag ctgaccctga atgggctcac agctgatgag gtaggagact
      961 gcctgaacgt ggaggcccag gtcagcatcg gtgcccaagc cagcgtctcc agtgaataca
     1021 aagcttgtga ggagaagaag aaacagcaca aaatggccac ctctttccac cagacctacc
     1081 gtgagcgtca cgtcgaagta cttggtggcc ctctggactc cacgcatgat ctgctcttcg
     1141 ggaaccaagc tacaccagag cagttctcaa cctggacagc ctcactgccc agcaaccctg
     1201 gtctggtgga ctacagcctg gagcccctgc acacattact ggaagaacag aacccgaagc
     1261 gggaggctct gagacaggct atcagccatt atataatgag cagagcccgg tggcagaact
     1321 gtagcaggcc ctgcaggtca ggccagcata agagtagcca tgattcatgc cagtgtgagt
     1381 gccaggattc aaaggtcacc aaccaggact gctgcccacg acagaggggc ttggcccatt
     1441 tggtggtaag caatttccgg gcagaacatc tgtggggaga ctacaccaca gctactgatg
     1501 cctacctaaa ggtcttcttt ggtggccagg agttcaggac cggtgtcgtg tggaacaata
     1561 acaatccccg gtggactgac aagatggact ttgagaatgt gctcctgtcc acagggggac
     1621 ccctcagggt gcaggtctgg gatgccgact acggctggga tgatgacctt cttggttctt
     1681 gtgacaggtc tccccactct ggtttccatg aggtgacatg tgagctaaac cacggcaggg
     1741 tgaaattctc ctaccatgcc aagtgtctgc cccatctcac tggagggacc tgcctggagt
     1801 atgcccccca ggggcttctg ggagatcctc caggaaaccg cagtggggct gtgtggtaac
     1861 ataataacat acaataacag ccggagagct gggtgtagta gcacacgcct ttaatcccag
     1921 catttgggag gcagagacag gtggatatct atgagttcga ggccagcctg ggtctacagg
     1981 gtctc
//