From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Bacillus subtilis 23S ribosomal RNA (23S rRNA) gene, 3' end; 5S
Date: 3 Jan 93 22:25:45 GMT
Distribution: bionet
Checksum: 49460 4

LOCUS       BACTGMD      1157 bp ds-DNA             BCT       24-DEC-1992
DEFINITION  Bacillus subtilis 23S ribosomal RNA (23S rRNA) gene, 3' end; 5S
            ribosomal RNA (5S rRNA) gene, complete; transfer RNA-Met (tRNA-Met)
            gene, complete; and transfer RNA-Asp (tRNA-Asp) gene, complete
            sequence.
ACCESSION   L08236
KEYWORDS    23S ribosomal RNA; 5S ribosomal RNA; transfer RNA-Asp;
            transfer RNA-Met.
SOURCE      Bacillus subtilis (strain 168T) DNA.
  ORGANISM  Bacillus subtilis
            Prokaryotae; Firmicutes; Endospore-forming rods and cocci;
            Bacillaceae.
REFERENCE   1  (bases 1 to 1157)
  AUTHORS   Rudner,R., Chevrestt,A., Bucholz,S.R., Studamire,B., White,A.M. and
            Jarvis,E.D.
  TITLE     Two tRNA Gene Clusters Associated with rRNA Operons rrnD and rrnE
            in Bacillus subtilis
  JOURNAL   J. Bacteriol. (1992) In press
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     rRNA            <1..78
                     /product="23S ribosomal RNA"
                     /gene="23S rRNA"
     rRNA            134..250
                     /product="5S ribosomal RNA"
                     /gene="5S rRNA"
     tRNA            265..341
                     /anticodon=(pos:unspecified,aa:Met)
                     /product="transfer RNA-Met"
                     /gene="tRNA-Met"
     tRNA            402..478
                     /anticodon=(pos:unspecified,aa:Asp)
                     /product="transfer RNA-Asp"
                     /gene="tRNA-Asp"
     terminator      486..510
     RBS             490..496
     CDS             499..1038
                     /label=ORF
                     /codon_start=1
                     /translation="MFSFFLCFLLLYLLLGVIAKLSLYREHLGYPAKFDKLGRAQIGI
                     SRVERRFWFMDEFDLIHSITPRTIHHSSVDVGIGDDAALYTAKHGVQEIVCVDTMVED
                     VHFKLHYSSPEDIGYKALAVNISDIAAMGGIPKFYLVSLAVPSKWTESEIKAMYEGMN
                     ELAKLYHMDLIGGVYSFYR"
BASE COUNT      310 a    230 c    290 g    327 t
ORIGIN      
        1 tgatcaggtt gataggtctg aggtgaagtg tggcgacaca tggagctgac agatactaat
       61 cgatcgagga cttaaccata tttttgaatg atgtcacatc tgttatctag ttttgagaga
      121 acactctcaa tttgtttggt ggcgatagcg aagaggtcac acccgttccc ataccgaaca
      181 cggaagttaa gctcttcagc gccgatggta gtcgggggtt tccccctgtg agagtaggac
      241 gccgccaagc aatactttta tcatcgcggg gtggagcagt tcggtagctc gtcgggctca
      301 taacccgaag gtcgcaggtt caaatcctgc ccccgcaacc aaatatatgg ttaaataagg
      361 agtgccctaa gggtgcaacc aaaataatcc tatacaaata tggtccggta gttcagttgg
      421 ttagaatgcc tgcctgtcac gcaggaggtc gcgggttcga gtcccgtccg gaccgccatt
      481 aatgtaaacg aaacgatcat gttttcgttt tttttgtgtt ttcttttact gtatcttctg
      541 cttggtgtca tagccaagct ttctttatat cgggaacatc ttggatatcc agcaaaattc
      601 gataaactag gaagagcaca aataggcata agcagggttg aaaggaggtt ttggttcatg
      661 gatgaatttg atttgataca tagcattact ccgcggacca ttcaccattc ttccgttgat
      721 gtaggaatag gtgatgatgc agcgctctac actgcgaaac acggcgttca ggaaattgtt
      781 tgtgttgata ctatggtaga agacgtgcac ttcaaattac actattcctc acctgaagat
      841 atcgggtaca aggcgttagc cgttaatata agtgatattg ccgcgatggg aggcattcct
      901 aagttttatc tggtatcact tgcggtacct tcaaaatgga ctgaatctga aatcaaggcg
      961 atgtatgaag gaatgaatga actggcgaaa ctatatcata tggatctgat tggcggcgta
     1021 tacagtttct accgctgaca aattagtcgt tacagtcact gtaatcggtg aaattgaaaa
     1081 gggacaggcc tgtttgcgca gcttggcaaa accgaatgat attgtgtttg taaccggtga
     1141 aatcggttct tctgcag
//