From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human pyruvate dehydrogenase alpha subunit mRNA, complete cds.
Date: 29 Sep 92 10:06:58 GMT
Distribution: bionet
Checksum: 13105     5

LOCUS       HUMPDHA      1419 bp ss-mRNA            PRI       29-SEP-1992
DEFINITION  Human pyruvate dehydrogenase alpha subunit mRNA, complete cds.
ACCESSION   J03575
KEYWORDS    pyruvate dehydrogenase.
SOURCE      Human foreskin fibroblast, cDNA to mRNA, clone pHPDA.
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 1419)
  AUTHORS   Koike,K., Ohta,S., Urata,Y., Kagawa,Y. and Koike,M.
  TITLE     Cloning and sequencing of cDNAs encoding alpha and beta subunits of
            human pyruvate dehydrogenase
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 85, 41-45 (1988)
  STANDARD  full staff_entry
REFERENCE   2  (bases 1 to 1419)
  AUTHORS   Koike,K., Urata,Y., Matsuo,S. and Koike,M.
  TITLE     Characterization and nucleotide sequence of human pyruvate
            dehydrogenase alpha subunit gene
  JOURNAL   Unpublished (1990) 12-4, Sakamoto-machi, Nagasaki-shi, 852, Japan
  STANDARD  full staff_entry
COMMENT
            [2]  revises [1].
            Draft entry and printed copy of sequence for [1] kindly provided by
            K.Koike, 11-APR-1988; and computer-readable copy for [2],
            12-MAR-1990.
FEATURES             Location/Qualifiers
     mRNA            <1..1419
                     /gene="PDHA"
     CDS             46..1218
                     /EC_number="1.2.4.1"
                     /product="pyruvate dehydrogenase alpha subunit"
                     /gene="PDHA"
                     /codon_start=1
                     /translation="MRKMLAAVSRVLSGASQKPASRVLVASRNFANDATFEIKKCDLH
                     RLEEGPPVTTVLTREDGLKYYRMMQTVRRMELKADQLYKQKIIRGFCHLCDGQEACCV
                     GLEAGINPTDHLITAYRAHGFTFTRGLSVREILAELTGRKGGCAKGKGGSMHMYAKNF
                     YGGNGIVGAQVPLGAGIALACKYNGKDEVCLTLYGDGAANQGQIFEAYNMAALWKLPC
                     IFICENNRYGMGTSVERAAASTDYYKRGDFIPGLRVDGMDILCVREATRFAAAYCRSG
                     KGPILMELQTYRYHGHSMSDPGVSYRTREEIQEVRSKSDPIMLLKDRMVNSNLASVEE
                     LKEIDVEVRKEIEDAAQFATADPEPPLEELGYHIYSSDPPFEVRGANQWIKFKSVS"
     sig_peptide     46..132
                     /gene="PDHA"
                     /codon_start=1
     mat_peptide     133..1215
                     /product="pyruvate dehydrogenase alpha subunit"
                     /gene="PDHA"
                     /codon_start=1
BASE COUNT      377 a    297 c    403 g    342 t
ORIGIN
        1 tcctgggttg tgaggagtcg ccgctgccgc cactgcctgt gcttcatgag gaagatgctc
       61 gccgccgtct cccgcgtgct gtctggcgct tctcagaagc cggcaagcag agtgctggta
      121 gcatcccgta attttgcaaa tgatgctaca tttgaaatta agaaatgtga ccttcaccgg
      181 ctggaagaag gccctcctgt cacaacagtg ctcaccaggg aggatgggct caaatactac
      241 aggatgatgc agactgtacg ccgaatggag ttgaaagcag atcagctgta taaacagaaa
      301 attattcgtg gtttctgtca cttgtgtgat ggtcaggaag cttgctgtgt gggcctggag
      361 gccggcatca accccacaga ccatctcatc acagcctacc gggctcacgg ctttactttc
      421 acccggggcc tttccgtccg agaaattctc gcagagctta caggacgaaa aggaggttgt
      481 gctaaaggga aaggaggatc gatgcacatg tatgccaaga acttctacgg gggcaatggc
      541 atcgtgggag cgcaggtgcc cctgggcgct gggattgctc tagcctgtaa gtataatgga
      601 aaagatgagg tctgcctgac tttatatggc gatggtgctg ctaaccaggg ccagatattc
      661 gaagcttaca acatggcagc tttgtggaaa ttaccttgta ttttcatctg tgagaataat
      721 cgctatggaa tgggaacgtc tgttgagaga gcggcagcca gcactgatta ctacaagaga
      781 ggcgatttca ttcctgggct gagagtggat ggaatggata tcctgtgcgt ccgagaggca
      841 acaaggtttg ctgctgccta ttgtagatct gggaaggggc ccatcctgat ggagctgcag
      901 acttaccgtt accacggaca cagtatgagt gaccctggag tcagttaccg tacacgagaa
      961 gaaattcagg aagtaagaag taagagtgac cctattatgc ttctcaagga caggatggtg
     1021 aacagcaatc ttgccagtgt ggaagaacta aaggaaattg atgtggaagt gaggaaggag
     1081 attgaggatg ctgcccagtt tgccacggcc gatcctgagc cacctttgga agagctgggc
     1141 taccacatct actccagcga cccacctttt gaagttcgtg gtgccaatca gtggatcaag
     1201 tttaagtcag tcagttaagg ggaggagaag gagaggttat accttcaggg ggctaccaga
     1261 cagtgttctc aacttggtta aggaggaaga aaacccagtc aatgaaattc aatgaaattc
     1321 ttggaaactt ccattaagtg tgtagattga gcaggtagta attgcatgca gtttgtacat
     1381 tagtgcatta aaagatgaat tattgagtgc ttaaagatt
//