HDTenEightyP/NetNews huggingface dataset:
From: mabee%ls_mail@SDSU.EDU
Newsgroups: bionet.jobs
Subject: name off mailing list
Date: 2 Sep 92 23:37:03 GMT
Distribution: bionet

Please take me off your mailing list.  Thanks.  Paula Mabee

Xref: sparky bionet.jobs:447 bionet.general:1627 sci.bio:3023 sci.bio.technology:236 sci.engr.biomed:502 misc.jobs.misc:6101 vmsnet.employment:80
From: hatamian@gandalf.rutgers.edu (ShAhin)
Newsgroups: bionet.jobs,bionet.general,sci.bio,sci.bio.technology,sci.engr.biomed,misc.jobs.misc,vmsnet.employment
Subject: MS-Biomed Seeking Employment
Date: 26 Aug 92 14:37:23 GMT
Followup-To: bionet.jobs
Distribution: usa
Organization: Rutgers Univ., New Brunswick, N.J.



Hello,

A friend has asked me to post this for her. Please respond directly to her
or via email to me for a complete resume.
____________________________________________________________________________

Hi,

I am graduating in December '92 from NJIT with a MS in Biomedical Engineering.
I am currently employed by a well-known hospital products manufacturing
company as a Product Development Engineer. I am conducting my thesis research
concurrently at this company.
please feel free to contact me for a resume and/or to discuss my qualifications
further.

Thank you for your time.

Please call Elli at (201)644-9174

____________________________________________________________________________
From: aq854@cleveland.Freenet.Edu (Lisa Craig)
Newsgroups: bionet.jobs
Subject: subscribe
Date: 2 Sep 1992 13:36:00 GMT
Organization: Case Western Reserve University, Cleveland, OH (USA)
Reply-To: aq854@cleveland.Freenet.Edu (Lisa Craig)
NNTP-Posting-Host: slc4.ins.cwru.edu


I would like to subscribe to this group
thanxs
Lisa
From: kaiser@soma.umdnj.edu (Brian Kaiser)
Newsgroups: bionet.jobs
Subject: Human Genetics
Summary: position available at the coriell institute. cytogenetics laboratory
Keywords: cytogenetics
Date: 2 Sep 92 17:28:11 GMT
Distribution: bionet

perform cytogenetic analyses on chromosomally abnormal human and animal 
cell cultures as well as human/rodent somatic cell hybrid cultures.  perform
special studies such as FISH, sister chromatid exchange, late labeling, etc.
conduct applied research to improve methods of cytogenetic characterization.
Contact the Coriell Institute for Medical Research, 401 Haddon Avenue, Camden,
New Jersey  USA  08103 EOE/AA (609) 966-7377    
Newsgroups: bionet.jobs
From: siemieni@med.umich.edu (David Siemieniak)
Subject: DNA Sequencing Technician Wanted
Originator: siemieni@sanger.hgp.med.umich.edu
Organization: University of Michigan - Medical Center
Date: Thu, 3 Sep 1992 20:06:25 GMT


We have an immediate opening for a person with DNA sequencing experience.  We are a core sequencing facility, part of the Human Genome Project, at the University of Michigan.  Must be familiar with DNA sequencing methods, operation of ABI sequencing machines, and pratical experience in a molecular biology laboratory.  Person will be involved with all phases of lab operations.  Experience with computer systems is essential.  


Please address all inquiries to:

David Siemieniak
Room 2568 MSRBII
University of Michigan
Ann Arbor, Michigan  48109-0652
E-mail: David.Siemieniak@med.umich.edu
From: godfrey@sscvx1.ssc.gov (LAURENCE GODFREY)
Newsgroups: bionet.jobs
Subject: Re: FW: SJapan: bio ctrl. research
Date: 31 Aug 92 20:49:00 GMT
Distribution: bionet
Organization: Superconducting Super Collider Laboratory
News-Software: VAX/VMS VNEWS 1.4-b1

In article <9208311903.AA19984@genbank.bio.net>, weigeld@STARBASE1.CALTECH.EDU ("WeigelD") writes...
> 
>L Godfrey writes:
> 
>>Regarding your choice of Germany or Japan, Germany is, in my opinion, the
>>most appalling country to work in.  I worked there for two years, and the
>>people are utterly humorless, racist, arrogant, and never admit to being
>>wrong.  Furthermore, Asians of all kinds, particularly Orientals (my wife
>>is one) are frequently threatened and often attacked by local neo-nazis.
>>Finally it is expensive, the food is revolting, the rents are high, cars
>>are outrageously expensive (particularly used cars), the bureacracy is
>>impenetrable and arrogant (especially the Immigration people) and the country
>>offers very little by way of amusement or entertainment.
> 
>What do you call somebody who condemns a people wholesale as racist?
> 
>A racist.
> 
>By the way, for an impenetrable Immigration bureaucracy, try the INS.
> 
>Detlef Weigel
>Caltech 
> 
> 
> 

The Germans are a nation, not a race.  Criticism of national characteristics
is legitimate, particularly if those characteristics are so prevalent and
objectionable as they are in the Germans.

I agree that the INS is awful (I am not American), but the US has compensations
whereas Germany has none.

LG
From: kaya@mscf.med.upenn.edu
Newsgroups: bionet.jobs
Subject: Molecular Biology/Philadelphia Area/Magainin Pharmaceuticals
Date: 2 Sep 92 18:05:40 GMT
Organization: Univ of Penn - Med School Computer Facility

SUMMARY;  A one year temporary position in the Molecular Biology Department at 
	Magainin Pharmaceuticals
KEYWORDS:  Molecular Biology/ Gene Expression/ Cloning/ Biology/ Philadelphia
	Pennsylvania/Temporary Position

A position is available immediately for an individual with a background in 
molecular biology.  A background in gene expression is desired, but not 
required.

The position is open to an individual with a college degree in the sciences.  
Individuals with post-graduate degrees are encouraged to apply.

Salary is competitive and commensurate with experience/degree.

For more information or to send an application, contact Dr. Angela Kaya or
Dr. Jon Williams at:

Magainin Pharmaceutical Inc.
5110 Campus Drive
Plymouth Meeting, PA 19462

TEL (215)941-5234 or (215)941-5238

FAX (215)941-5399

or EMAIL to KAYA@MSCF.MED.UPENN.EDU

Applications should include a letter of reseach interests and experience, 
curriculum vitae and names and phone numbers of at least two references.

Magainin Pharmaceuticals Inc. is a biopharmaceutical company engaged in the 
research and evelopment of a new family of host-defense peptides for the 
treatment of infection and certain cancers and the promotion of wound healing.

Magainin Pharmaceuticals Inc. is an equal opportunity employer.
From: gvh@se.ki.csb
Newsgroups: bionet.jobs
Subject: Biocomputing-Stockholm
Date: 27 Aug 92 09:47:24 GMT
Distribution: bionet
Original-To: biojobs@uk.ac.daresbury
Original-Sender: gvh@se.ki.csb.ondine





The Karolinska Institute at NOVUM Research Park
Center for Structural Biochemistry

ADVANCED POSTDOC

Biocomputing


We have an immediate opening within the Swedish Genome Research Program for
an advanced postdoc with a strong background in biocomputing and
biosequence analysis. The position offers oppurtunities for independent
research in a multidisciplinary environment ranging from structural
biochemistry (X-ray crystallography, NMR, molecular modelling) to molecular
and cell biology, and with good computing facilities (VMS, UNIX, parallel
computing). We can offer an initial 3-year appointment, with possibilities
for extension.


Please address all inquiries to:

Dr. Gunnar von Heijne, Center for Structural Biochemistry, NOVUM,
S-141 57 Huddinge, Sweden.
Phone: Int+46-8-608 91 31
Fax: Int+46-8-774 55 38
E-mail: gvh@csb.ki.se
From: lcl@splinter.coe.northeastern.edu (Lisa C. Lewis)
Newsgroups: bionet.jobs
Subject: Re: have this stopped simply
Date: 2 Sep 92 00:50:03 GMT
Reply-To: lcl@meceng.coe.northeastern.edu
Followup-To: alt.flame
Distribution: bionet
Organization: College of Engineering, Northeastern University
In-reply-to: godfrey@sscvx1.ssc.gov's message of 1 Sep 92 12:22:00 GMT


Ok, guys, enough is enough.  Much as I enjoy a good flame-fest, it's time
to move the discussion to another group.  Or you can just email each other
and cc it to all involved in the discussion.  Of course, should you later
want to shout arguments biological, this is just the place! ;^)

We now return to your regularly scheduled (and far less colorful)
programming... 


Peace,
 Lisa Caroline


--
Lisa Caroline Lewis   		    Our country, right or wrong.  
lcl@csa.bu.edu      -or-	    When right, to be kept right; 
lcl@meceng.coe.northeastern.edu     when wrong, to be put right.
                  	             - Carl Schurz, January 17, 1872
From: kristoff@genbank.bio.net (David Kristofferson)
Newsgroups: bionet.jobs
Subject: Re: FW: SJapan: bio ctrl. research
Date: 3 Sep 92 02:12:57 GMT
Distribution: bionet
Organization: GenBank Online Service

Discussions of national character traits are not in the charter of
EMPLOYMENT/bionet.jobs.  I've been out of town, otherwise I would have
jumped on this one much earlier.

				Sincerely,

				Dave Kristofferson
				BIOSCI/bionet Manager

				kristoff@genbank.bio.net
From: knox@spruce.gsfc.nasa.gov (Robert Knox)
Newsgroups: bionet.plants
Subject: Andrew and Fairchild Gardens
Date: 28 Aug 92 18:37:07 GMT
Reply-To: knox@spruce.gsfc.nasa.gov
Organization: NASA/GSFC

As a small footnote, compared to billions of dollars of 
destruction in S. Florida, Fairchild Gardens was within
the area of severe damage from Hurricane Andrew (in think
in Coral Gables).  Anyone have information on how Fairchild
and its world-renown living collection of palms weathered
the storm?

An unrelated query:  Though it may seem insensitive to
consider this issue with tens of thousands of Floridians
facing dehydration, food shortages, and huge personal 
losses, I'll raise the question now anyway.  What is the 
wisdom of publicly funding rebuilding on a fragile coastal
zone, especially as we move into the more intense portion
of the long-term hurricane cycle for Atlantic hurricanes?  
Are all citizens being asked to accept and cover risks 
from individual decisions to build on ocean shores?  
(Hurricanes Hugo, Bob, and Andrew are believed to represent
a return to a high frequency of powerful, destructive 
storms hitting the U.S. East Coast, last experienced in 
the 1950's and early 1960's.)

Note: I am *not* questioning the vital immediate need to
provide water, food, shelter, and basic services to the
people and region, as quickly as possible.  Rather the 
question is about federal coverage of uninsured losses, 
subsidized loans, etc.  


- Bob Knox
- Biospheric Sciences Branch, Code 923.0
- NASA/Goddard Space Flight Center, Greenbelt, MD 20771 
- knox@spruce.gsfc.nasa.gov      | BITNET: knox@ricevm1
--------------------------------------------------------
Maryland has its own ocean-side heavy development, at 
Ocean City, on a barrier island next to an inlet and harbor 
cut during the last major hurricane cycle!  There's a 
pretty good chance we'll be asked--through taxes and
insurance premiums--to fund rebuilding all those hotels 
and condos some time in the next 20 years.
From: rjsalvad@iastate.edu (Ricardo J Salvador)
Subject: Maize Handbook
Organization: Iowa State University, Ames, IA
Date: Fri, 4 Sep 1992 16:41:52 GMT

On the subject of new publications, Springer-Verlag has scheduled
release of "The Maize Handbook" for this Fall.  Their publicity
description:

---
The Maize Handbook
Michael Freeling and Virginia Walbot, editors

This important book will be an indispensible resource for all students
of maize.  Maize has been prominent as a model for research in genetics
for many decades, and has a collection of methods and mutants unmatched
among plants.  New developments in cell and molecular biology have kept
maize at the forefront of plant research.  The Maize Handbook has been
compiled by a distinguished group of maize researchers to serve as  a
community resource.  The book presents dozens of current protocols in
all areas of maize biology, and includes major sections on development,
morphology and cell biology which will be invaluable to workers needing
detailed guides to maize at the cell, tissue and molecular levels.

Approx. 500 pp./Softcover, comb bound $79 (tentative)/ISBN 0-387-97826-7

Contents are organized as follows:

I.   Development and Morphology, edited by Scott Poethig

II.  Cell Biology, edited by Ann Sylvester

III. Genetics Protocols, edited by Ron Phillips and Jim Birchler

IV.  Molecular Biology Protocols, edited by Sue Wessler

V.   Cell Culture Protocols, edited by Ron Phillips

Springer-Verlag can be contacted by calling toll-free: 1-800-SPRINGER in
the continental U. S.  (777-4643 in New Jersey, 201-348-4033 in Canada).
From: bwilliam@oyster.smcm.edu (Bill Williams)
Newsgroups: bionet.plants
Subject: Re: Andrew and Fairchild Gardens
Date: 31 Aug 92 12:49:07 GMT
Organization: St. Mary's College of Maryland
X-UserAgent: Nuntius v1.1.1d9
X-XXMessage-ID: <A6C78DABC80101D2@williams.smcm.edu>
X-XXDate: Mon, 31 Aug 92 13:49:47 GMT

In article <1992Aug28.183707.22152@nsisrv.gsfc.nasa.gov> Robert Knox,
knox@spruce.gsfc.nasa.gov writes:
> What is the 
>wisdom of publicly funding rebuilding on a fragile coastal
>zone, especially as we move into the more intense portion
>of the long-term hurricane cycle for Atlantic hurricanes?  

I highly recommend John McPhee's "The Control of Nature" as an
interesting source of light on the general subject of living where nature
poses obvious and immediate threats to one's livlihood.  McPhee is pretty
neutral (I think) about the various precarious situations that people put
themselves in, but the stories seem to speak for themselves.  Personally,
as a displaced northern Californian I've always been thoroughly
scandalized by the people in the San Joaquin/Sacramento River Delta, who
are periodically flooded out, loose everything, and then are paid
millions by various government agencies to rebuild so they can be flooded
out again!

The situation in Florida seems somewhat different, though:  the
Washington Post, at any rate, said that huge percentages of the destroyed
buildings didn't even come up to the codes already in place.  That's just
plain irresponsible (on the part of the builders and enforcement
officials, of course, not the displaced home-owners).
From: ES@UK.AC.AFRC.JII
Newsgroups: bionet.plants
Subject: R: RNA from seeds, some more problems
Date: 31 Aug 92 16:16:00 GMT
Distribution: bionet
Original-To: PLANTBIO@UK.AC.DARESBURY

From:	CBS%UK.AC.NSFNET-RELAY::EDU.OKSTATE.UCC.MVS::BTNYLAC 28-AUG-1992 02:38:41.58
To:	es
CC:	
Subj:	RNA from siliques

Via: UK.AC.NSFNET-RELAY; Fri, 28 Aug 92   2:38 GMT
Received: from dlgm.daresbury.ac.uk by sun3.nsfnet-relay.ac.uk 
          with Internet SMTP id <sg.26173-0@sun3.nsfnet-relay.ac.uk>;
          Fri, 28 Aug 1992 02:38:16 +0100
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          Fri, 28 Aug 92 02:32:35 BST
Date: Fri, 28 Aug 92 02:32:34 UT
Message-Id: <92728023234.MIN-LJMBa26691.bionet-news@uk.ac.daresbury>
From: BTNYLAC@EDU.OKSTATE.UCC.MVS
Reply-To: BTNYLAC@EDU.OKSTATE.UCC.MVS
Original-Sender: "bionet.genome.arabidopsis mail newsgroup" <bionet-news@uk.ac.daresbury>
To: "bionet.genome.arabidopsis mail newsgroup" <bionet-news@uk.ac.daresbury>
Subject: RNA from siliques
X-Article-Number: bionet.genome.arabidopsis Msg # 178
Comments: List problems/queries to <biosci@daresbury.ac.uk>
Comments: To mail both the group and netnews send to (arab-gen@daresbury.ac.uk)

Brian,
I also got a rusty color contaminant in silique
RNA preps with the phenol/LiCl method.  I switched
to guanidinium thyocyanate/ CsCl and got clean RNA.
Some references for this method are:
Chirgwin et al Biochemistry 18:5294-99 (1979)
Kopczynski and Scandalios MGG 203:185-8 (1986)
and Current Protocols in Molecular Biology 4.2
Hope this helps.
P.S. The rusty contaminant hung in the wells of
agarose gels and the RNA still ran fine.
Linda Castle, Botany, Oklahoma State University
Mon, 31 Aug 1992 10: 46:54 +0100
               Mon, 31 Aug 1992 10: 46:34 +0100
               Mon, 31 Aug 1992 13: 36:09 +0100
From: suter@de.dbp.MPG.MPIZ-KOELN.VAX
Newsgroups: bionet.plants
Subject: rna from seeds, some more problems
Date: 31 Aug 92 12:36:09 GMT
Distribution: bionet
Content-Identifier: 12
Original-To: plantbio@uk.ac.daresbury
Conversion: Prohibited


 brian tague wrote:

<Subj:	Isolation of RNa from seeds
<
<Authorizers: S=TAGUE;OU=FRODO;OU=MGH;O=HARVARD;P=edu;A=dbp;C=de
<Reply-To: S=TAGUE;OU=FRODO;OU=MGH;O=HARVARD;P=edu;A=dbp;C=de
<Comments: To mail both the group and netnews send to (arab-gen@daresbury.ac.uk)
<
<==================
<Does anyone have a reliable method for the isolation of RNA from dry seeds
<and/or yellow siliques. With the standard RNA prep (SDS/LiCl/phenol/chloroform)
<some kind of contaminant comes through with the RNA. Procedures for the removal
<of polysaccharides (1M NaCl ppt @ 0C, acid ppt) don't remove it. Any hints or
<suggestions would be greatly appreciated. Thanks in advance.
<-- Brian W. Tague

we are currently having the same problem with RNA isolated from tobacco seeds:
mRNA is prepared according to your method and purified further with dyna-
beads. Resulting RNA is rather brown (in contrast to leaf rna) and cannot
be used to efficiently produce a cDNA library (again in contrast to leaf rna).

is there some oxidating agent in seeds ? we notice that seeds, freshly opened 
are white, but turn brownish within 30 minutes.

any hints welcome.

clemens
----------------------------------> address <----------------------------------
Clemens Suter-Crazzolara, PhD
Max-Planck-Institut fuer Zuechtungsforschung
Abteilung Genetische Grundlagen der Zuechtungsforschung
Carl-von-Linne Weg 10
5000 Koeln 30
Tel. xx49-221-5062.221           fax. xx49-221-5062.21
suter@vax.mpiz-koeln.mpg.dbp.de
-------------------------------> end of message <------------------------------
Newsgroups: bionet.plants
From: br105@cleveland.Freenet.Edu (Jeffrey A. Del Col)
Subject: Epsom Salts to promote basal canes on roses?
Reply-To: br105@cleveland.Freenet.Edu (Jeffrey A. Del Col)
Organization: Case Western Reserve University, Cleveland, OH (USA)
Date: Thu, 27 Aug 92 12:37:53 GMT


I would be grateful for any information about the use of magnesium 
sulfate solution (epsom salts) to stimulate the growth of new
basal canes on rose bushes.  I read a passing reference to this 
method in one of the Taylor's Guide series, but there was nothing
specific about what concentration to use or when it ought to
be applied.  It also said nothing about why this ought to work.
Any reports of experience with this or explanations of why it 
should or shouldn't work would be most welcome.

J. Del Col
-- 
Jeff Del Col      *       "I'm never lonely,...living in my heavily      
A-B College       *   populated solitude, a harum-scarum of infinity        
Philippi, WV      *   and eternity, and Infinity and Eternity seem to          
                  *   take a liking to the likes of me." Bohumil Hrabal.
From: mwfolsom@hydra.unm.edu (Mike Folsom)
Newsgroups: bionet.plants
Subject: Re: Bionet Snooze
Date: 27 Aug 92 16:54:35 GMT
Distribution: bionet
Organization: University of New Mexico, Albuquerque

In article <01GO2QM9IS0G8WXR3D@PEARL.TUFTS.EDU> GELLMORE@PEARL.TUFTS.EDU (BIOHEAD) writes:
>Tony comments that the bionet group seems to "wake up" when discussing
>e-mail policies rather than plant topics. I disagree. I think that members
>of bionet POST their responses about e-mail policies to the general list, but
>that specific plant-oriented discussions tend to occur between individual
>users. 
>
>When I type in the "reply" command, the 'puter automatically provides the
>specific address of the sender, NOT the general genbank.bio.net address.
>Thus, my most animated discussions have been entre deux, and I suspect
>others have shared this experience.
>
>George Ellmore
>Biology Dept.
>Tufts University
>Medford, MA  02155

I agree.

Just because a person doesn't posting does not mean that they aren't there.

To risk Tony's ire about non-relevant things on bionet.plants -

Re: the "reply" command.  f or F is just as easy to use, the "follow up"
command, as is r or R and its much more interesting to the rest of us. ;-)

Later -

Michael
From: moysan@irisa.fr (Erwan Moysan)
Newsgroups: bionet.plants
Subject: User address needed. Scotsmen only.
Date: 26 Aug 92 09:01:30 GMT
Reply-To: moysan@irisa.fr
Organization: IRISA, Rennes, France


Hi bio netters ! It might be the wrong newsgroup, but it's urgent !

I've been in contact (by email) with somebody who's working in a
genetics lab in Edimburgh (Scotland). His name is John Love and his
email address is completely different (someone else address ?).
Unfortunately, I've lost it. Does anyone on the net know him and
his address ? Thank you for replying by email.

Erwan Moysan

+-----------------------------+                         
|       - Erwan Moysan -      |  !   !   ! xxxxxxxxxxxxx
|  - Erwan.Moysan@Irisa.Fr -  |    !   !   xxxxxxxxxxxxx 
| Bureau AB 306 - 99.84.72.90 |  !   !   ! xxxxxxxxxxxxx
|    Fax: (33).99.38.38.32    |  xxxxxxxxxxxxxxxxxxxxxxx         
| IRISA - Campus de Beaulieu  |  xxxxxxxxxxxxxxxxxxxxxxx
|    35042 RENNES CEDEX       |  xxxxxxxxxxxxxxxxxxxxxxx 
|      BREIZH - FRANCE        |  xxxxxxxxxxxxxxxxxxxxxxx
+-----------------------------+ 
Newsgroups: bionet.plants
From: claird@NeoSoft.com (Cameron Laird)
Subject: Re: Andrew and Fairchild Gardens
Organization: NeoSoft Communications Services -- (713) 684-5900
Distribution: bionet
Date: Tue, 01 Sep 1992 14:02:19 GMT

In article <1992Aug31.101420.20725@gserv1.dl.ac.uk> ajt@uk.ac.sari.rri writes:
			.
			.
			.
>Is this return to high frequency storms associated with global warming ??
As others have commented, it's not
clear that this is a well-posed
question.
>
>And, what did the hurricane do to the fragile ecosystem of the everglades ??
Hurricanes are *part* of "the
fragile ecosystem ..."  That's far
from a complete answer, of course,
but perhaps it's appropriate to
note at least that.
>
>The UK was lashed by the tail end of these storms over the weekend and THAT
>was bad enough.  As you know, I am interested in the ability of crops to
>resist 'lodging' damage.  The crops in the North East of Scotland have been
I didn't know, but perhaps I haven't
been reading bionet.plants long enough.
Please, tell more:  do Scots farmers
interplant barley with oats to reduce
lodging?  What other crops have problems
with lodging?  What approaches--plant
breeding?  other?--are taken to improving
European cultivars in these regards?
-- 

Cameron Laird
claird@Neosoft.com (claird%Neosoft.com@uunet.uu.net)	+1 713 267 7966
claird@litwin.com (claird%litwin.com@uunet.uu.net)  	+1 713 996 8546
From: pbacon@vax.oxford.ac.uk
Newsgroups: bionet.plants
Subject: energy and rapeseed
Date: 25 Aug 92 12:35:40 GMT
Organization: Oxford University VAX 6620

There is a very heated debate raging in Europe at the moment regarding so-
called Rape methyl-esters and their value for replacing diesel fuel.  Oil
seed rape is grown extensively and has been overproduced due to subsidy
under the Common Agricultural Policy - excess crops are now being used for
fuel.  RMEs are thought to have many ecological advantages but economically
they remain uncompetitive with fossil fuels.  Several farm equipment 
manufacturers offer models with engines adapted to run on RMEs and I
have read that a fleet of delivery vehicles has been commissioned in 
Germany to run on RMEs. 

Regarding erucic acid,  I understood that it is poisonous to humans which
must be a good reason for breeding it out of crop lines.   However, there
are high erucic acid cultivars available and they are advertised as such
so there must be a commercial use for high erucic acid rapeseed oil.