From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Synechococcus sp. photosystem I protein (psaE) gene complete cds
Date: 21 Aug 92 10:12:57 GMT
Distribution: bionet
Checksum: 64631     5

LOCUS       SYOPSAEGLT   1621 bp ds-DNA             BCT       21-AUG-1992
DEFINITION  Synechococcus sp. photosystem I protein (psaE) gene complete cds
            and glutamyl tRNA ligase (gltX) gene, 5' end.
ACCESSION   M99379
KEYWORDS    glutamyl tRNA ligase; photosystem I protein;
            photosystem I reaction center.
SOURCE      Synechococcus sp. (library: PCC 7002) DNA.
  ORGANISM  Synechococcus sp.
            Prokaryotae; Gracilicutes; Oxyphotobacteria; Cyanobacteria;
            Chroococcales.
REFERENCE   1  (bases 1 to 1621)
  AUTHORS   Zhao,J., Snyder,W., Muhlenhoff,U., Rhiel,E. and Bryant,D.A.
  TITLE     Cloning and characterization of the psaE gene of the cyanobacterium
            Synechococcus sp. PCC 7002: characterization of a psaE mutant and
            overproduction of the protein in Escherichia coli
  JOURNAL   Unpublished (1992)
  STANDARD  full staff_entry
FEATURES             Location/Qualifiers
     RBS             29..34
     CDS             46..663
                     /note="putative"
                     /codon_start=1
                     /translation="MTAHNFFWDGDWFSPQASQDQNLIAQLDFVPGLRELLFIRQAHA
                     LEHGTVWVLTELAERYPTAWRHHYAELSGMSTGQGFYLFGGVDKTDLYRAVSTAGDRL
                     RSGEWNLAIHPRCGTNISVTLLLTAGLAGGAAWLLPKDPLTQLFGMGTAAATAWAIAP
                     DLGKYAQQHITTAIPLNLALEEIQENTDESGNPSHFVRLRWQDAQ"
     RBS             749..755
                     /gene="psaE"
     CDS             763..975
                     /function="possible role in cyclic electron transport"
                     /gene="psaE"
                     /evidence=EXPERIMENTAL
                     /codon_start=1
                     /translation="MAIERGSKVKILRKESYWYGDVGTVASIDKSGIIYPVIVRFNKV
                     NYNGFSGSAGGLNTNNFAEHELEVVG"
     stem_loop       1017..1043
                     /gene="psaE"
     RBS             1129..1134
                     /gene="gltX"
     CDS             1143..1621
                     /function="amino-acylates tRNA-Glu"
                     /product="glutayml tRNA ligase"
                     /gene="gltX"
                     /codon_start=1
                     /translation="MTVRVRIAPSPTGNLHIGTARTAVFNWLFAHHHGGTFVLRVEDT
                     DLERSKPEYTENIKTGLQWLGLHWDEGPFFQTQRLDQYKAAIQTLLDQGLAYRCYCTP
                     AELEAMREQQKANNQAPRYDNRHRNLTEAQRAEFEAQGRKPVIRFKIDDAQQIVWQDL
                     "
                     /partial
BASE COUNT      446 a    419 c    360 g    396 t
ORIGIN
        1 aagctttgat ggacaaaatt ttttcagtaa aggataacaa cagcaatgac agcgcataat
       61 tttttctggg atggtgactg gttttcaccg caggccagtc aagatcaaaa tttaatcgcg
      121 caattggatt ttgtgcccgg tctgagggag ctgctcttta ttcgccaggc ccatgccctc
      181 gaacatggaa cggtctgggt gcttacagaa ttggcagaac gctacccaac ggcctggcgt
      241 caccactacg cggaattaag tggaatgtcc acggggcaag ggttttattt atttggtggc
      301 gtggataaaa ccgatcttta ccgggcggtc agtacagccg gcgatcgcct ccggtctggg
      361 gaatggaatt tagcgattca tcctcgctgc ggcaccaata tctctgtcac tctactacta
      421 acagcaggat tagctggtgg agccgcttgg ttgctcccca aagatcccct cacccaactc
      481 tttggtatgg ggactgctgc tgccacagcc tgggcgatcg cccctgatct cggtaaatat
      541 gcccaacagc acatcaccac cgcgattccc ctcaatctcg ccctcgaaga aatccaagaa
      601 aacacggacg agagtggaaa tccaagccat tttgtccgat tacggtggca ggacgcgcaa
      661 taaaacttaa acacttcaag tgcctgaaaa cccctatccg atagtaggct gttatctaag
      721 tctaactaca ctctttagta atcgctttaa aggaacataa gcatggctat cgaacgcgga
      781 tccaaagtta aaattctccg gaaagaatcc tactggtacg gcgatgtcgg cacagtagca
      841 agcattgata aaagcggtat tatctatccc gtcattgttc gctttaacaa agtcaactac
      901 aacggcttta gcggtagtgc tggtggtcta aacaccaaca acttcgctga gcatgaactc
      961 gaagtcgttg gctaagccct ctgtctctct ttttaaaaac ccatttttga tattaaacac
     1021 acaaccaaca tcgtctcttt gcggggcggt gtttttatat gccccttcaa attcctaaag
     1081 gacaatggga aaatagccac cacaaagatt aaattaaacc actacaacaa aggattaacc
     1141 ccgtgactgt tcgcgttcgt attgccccaa gtcccaccgg aaatctccac atcgggacag
     1201 ctagaactgc tgtctttaat tggctttttg cccatcacca tggcggtacc tttgtcctcc
     1261 gggttgaaga taccgatttg gaacggtcta agcccgaata caccgaaaac attaaaacgg
     1321 gtctccaatg gctcggtttg cactgggatg aaggcccttt tttccaaacc caacggctgg
     1381 atcaatacaa agcagcaatc caaacccttt tagatcaagg tcttgcctat cgttgttact
     1441 gtacccccgc agaacttgag gcgatgcggg aacaacaaaa agcgaataat caagcgcccc
     1501 gctatgacaa tcgtcaccgc aacttaacgg aggcgcagcg agctgaattt gaagcccaag
     1561 gtcgtaaacc cgtgattcgc ttcaaaattg acgatgccca gcaaatcgtt tggcaagatc
     1621 t
//