From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: S.pombe pcn gene for proliferating cell nuclear antigen
Date: 3 Jan 93 22:22:58 GMT
Distribution: bionet
Checksum: 59668 5

LOCUS       YSPPCNG      2190 bp ds-DNA             PLN       23-DEC-1992
DEFINITION  S.pombe pcn gene for proliferating cell nuclear antigen
ACCESSION   X54857
KEYWORDS    cell proliferation; nuclear antigen;
            proliferating cell nuclear antigen.
SOURCE      Schizosaccharomyces pombe DNA.
  ORGANISM  Schizosaccharomyces pombe
            Eukaryota; Fungi; Eumycota; Hemiascomycetes; Endomycetales;
            Schizosaccharomycetoideae.
REFERENCE   1  (bases 1 to 2190)
  AUTHORS   Waseem,N.H., Labib,K..., Nurse,P. and Lane,D.P.
  TITLE     Isolation and analysis of the fission yeast gene encoding
            polymerase delta accessory protein PCNA
  JOURNAL   EMBO J. 11, 5111-5120 (1992)
  STANDARD  full automatic
COMMENT     *source: clone_library=genomic cosmid, lorist4/S.pombe; *source:
            clone=ICRFc60D1016;
               From EMBL    entry SPPCNG;  dated 17-DEC-1992.
FEATURES             Location/Qualifiers
     exon            <845..925
                     /number=1
     mat_peptide     join(845..925,1037..1735)
                     /product="proliferating cell nuclearantigen"
                     /gene="pcn"
                     /codon_start=1
     CAAT_signal     177..182
     intron          926..1036
                     /number=1
     exon            1037..>1738
                     /number=2
     CDS             join(845..925,1037..1738)
                     /gene="pcn"
                     /product="proliferating cell nuclearantigen"
                     /codon_start=1
                     /translation="MLEARFQQAALLKKLLDAIKELVTDANFDCNDNGISLQAMDSSH
                     VALVSMLIKSDGFEPYRCDRNIALGINLNALSKVLRCAQNEDLVTLKAEDTPEVLNLV
                     FESEKNDRISDYDVKLMDIDQEHLGIPDIEYDATITMPAAEFQRITRDLLTLSDSVTI
                     NASKEGVRFSCKGDIGNGSTTLKQHTDLSDQDQSIEISLTQAVTLTFSLKYLAQFTKA
                     TPLATRVTLSMSNDVPLLVEYKMESGFLRFYLAPKIGEEDEE"
BASE COUNT      725 a    365 c    361 g    739 t
ORIGIN      
        1 aattcttgtt aacgtaactg taatctacga atattgagaa gaaaaaaaaa cgaatgaatt
       61 tgttgtgaat acatattttt tccttagcga acaagtatac ttcattgtta ttttcatacc
      121 tctttagtct tatttaaacc agcaataaaa ttcgttttaa gttttccgtg ttaataccaa
      181 ttaaaataac gtttttttca atcattttat atgtcattgc aacgtgaaaa taagttaatg
      241 gaaatttcgg tagcagctaa tatggaaaat gtacaattta cttgatacat tagtgtagcc
      301 taaattttat ttgatcctgg aaagtatatt aaggtagaac gcgaaagctc tcctagttta
      361 cgccgtaaat tgtaatgact cgctgattac atttggctta ttataatccg tatagctaga
      421 aaatttcgat aactgaatat tgcttcttta aaaagacata aatagaaccg tgaattagta
      481 cagcaaacga aagataaaaa tttcatagta acatgcaaat actatttttt cagttagttt
      541 tccataattt tgtataatat ttcatatcaa acgcatctaa aaagtacgga cgaaattact
      601 tgctattgta aaaataaaaa aaataacaaa acaaattata ataacgtgaa ctgctatttt
      661 ggtctcatat atgaaaggtt cttgagattt tttacgttgt cagctagcaa ttttacgcac
      721 actattctgc ttgacgcgct ctttgactac ttcccaaaag ccaactacaa aaggtttttg
      781 cagtaacctt tgttagctat tctttatatc tcttatctag cttgtataaa atataattac
      841 gactatgctt gaagctagat ttcagcaggc tgctttgttg aaaaagcttc tcgatgccat
      901 caaggagtta gttacagatg ctaatgtatg taaggggcag catataaata aagatgctca
      961 acgacaaagc ctttcttttt tttaaaaaaa atatatttat taacttcttt tgaatgattc
     1021 tctaacctca taacagtttg attgcaatga caacggtatc tctctgcagg ctatggatag
     1081 ttctcatgta gcccttgttt ctatgcttat taaatcagat ggatttgaac catatcggtg
     1141 tgatagaaat attgctttag gtataaatct gaacgcctta tcaaaggtct tacggtgtgc
     1201 ccaaaacgag gatttggtta ctttaaaggc tgaagataca cctgaggtat tgaatttggt
     1261 tttcgagagc gagaaaaatg acaggatctc tgattatgat gttaaattaa tggacattga
     1321 tcaagaacac ttgggtatac cagatatcga atacgatgct actattacta tgcctgctgc
     1381 cgaatttcaa cgcattactc gagatttatt aactttgagt gattcagtaa caatcaatgc
     1441 tagtaaagag ggtgttcgtt ttagctgcaa gggagatatt ggtaatggta gtaccacgct
     1501 gaaacaacat accgatcttt ctgaccaaga tcaaagtatc gaaatttctc ttacccaagc
     1561 tgtaacactt acgttttctt tgaaatattt agcccagttc actaaggcta ctcctttagc
     1621 tacccgagtc acactttcaa tgagcaatga tgttccactt cttgtggagt ataaaatgga
     1681 atccggattt ttacgatttt atcttgcccc aaaaattggt gaggaggatg aggagtagat
     1741 tacggaatca atatctttta gtaatctctt tgcaattgac gagctagcat cattataaac
     1801 atacttttgt ttacttttta attcaacctt ttttgtactg ctccttccct aacattcata
     1861 tccaaacagg caaaatacca gcttttactt aaaaaaaatc aaattcttta gaaccaacaa
     1921 actgaagggt agaaaaactt gtgttcttag tgatgatttt tcacagataa cttcgtaatc
     1981 atctgctgta gcttgttgat ttaagtctag tacatgatat aatacattga tatgtaaatt
     2041 agcttaattc tgagaattta ttctaactga taattgtata cttatatggc taaccgcgta
     2101 agagcctcgg agggacagtt cgcccaaata ctaccaacgc taccaccagt agcagcaagg
     2161 cgtaaaagtt acttaaagaa gatagaattc
//