From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human cystic fibrosis transmembrane conductance regulator gene, 5'
Date: 26 Jan 93 05:47:44 GMT
Distribution: bionet
Checksum: 22221 8

LOCUS       HUMCFTC      4389 bp ds-DNA             PRI       25-JAN-1993
DEFINITION  Human cystic fibrosis transmembrane conductance regulator gene, 5'
            end.
ACCESSION   M58478 M58479
KEYWORDS    cystic fibrosis transmembrane conductance regulator;
            transmembrane protein.
SOURCE      Human DNA.
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 4389)
  AUTHORS   Yoshimura,K., Nakamura,H., Trapnell,B.C., Dalemans,W., Pavirani,A.,
            Lecocq,J.-P. and Crystal,R.G.
  TITLE     The cystic fibrosis gene has a housekeeping-type promoter and is
            expressed at low levels in cells of epithelial origin
  JOURNAL   J. Biol. Chem. 266, 9140-9144 (1991)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     GC_signal       2876..2881
                     /gene="CFTR"
     GC_signal       3492..3497
                     /gene="CFTR"
     GC_signal       3571..3576
                     /gene="CFTR"
     CAAT_signal     3658..3662
                     /gene="CFTR"
                     /note="CCAAT-like motif"
     promoter        3724..3825
                     /gene="CFTR"
                     /evidence="EXPERIMENTAL"
                     /note="minimum promoter"
     CAAT_signal     3766..3770
                     /gene="CFTR"
                     /note="inverted CCAAT motif"
     CAAT_signal     3774..3778
                     /gene="CFTR"
                     /note="CCAAT-like motif"
     mRNA            3826..4389
                     /gene="CFTR"
                     /evidence="EXPERIMENTAL"
                     /partial
     exon            3826..3949
                     /number=1
                     /gene="CFTR"
                     /evidence="EXPERIMENTAL"
     intron          3950..>4389
                     /number=1
                     /gene="CFTR"
                     /evidence="EXPERIMENTAL"
     CDS             3897..3949
                     /gene="CFTR"
                     /partial
                     /product="cystic fibrosis transmembrane conductance
                     regulator"
                     /codon_start=1
                     /translation="MQRSPLEKASVVSKLFF"
BASE COUNT     1284 a    803 c   1004 g   1298 t
ORIGIN      chromosome 7q31
        1 gaattcaaag gaaaacataa gatgcaattc gtgcctccaa ggaggttgta gggaagaggg
       61 gttatgaatg tatgtaaata gaagttggtg tgcgtgtgtg tttataaaca gaattgtcag
      121 accaaacatt attttggaag cagtaaaagt aaactagaat ctggcctagt catgtcccag
      181 gacacctctt tcaagtcctg aaacatcttt gtaagactgt aatgtgtgtt tacatcctag
      241 gtaatcactg tggcccactg ttgaagagct gtggctgttc ttacccttct agttagataa
      301 acttataagc acaaccagac tacatatatg aagctgaaga gaccttgtct ttttttaacg
      361 agcttttctt cccgatagga gtgactattt cttttcttct tccacatttt caggttttag
      421 tgtacttgtg attgctaccc acttatcact attaaagtct actcaggaga gaatctgaga
      481 aacactctca aattaagttg aacatgatgg ataagtaaag tattgtgaaa gttcactctc
      541 atgatttcta atggtgaaac ctggcagggt gactaatctt tgacgagaag gttatcactt
      601 ataatctttc atatattgag atcatttgta agaagcaccc agcacattgc tgaacacaaa
      661 gtaggtatta aataaatgtt ggcttccttt tctcctactc atcctcgctc ttctttttaa
      721 tataccttta aaatgatgcc acagaaatgg ccacccaatc ttctatattt aaggtcagtt
      781 cttgcattag gaaattctat aggggaagta tgtgaagtat gtgtagtcag tcattaaatg
      841 cttgggctct ggccacagat tgtttaggtt taaatcccag tttcctcttt tattattaat
      901 tgtgcaactt gcttgggaaa acatgaaact tgtttttcct caggttcatt atctgtaata
      961 tatagtgaat gaagaagttt cctgtcccat gaaggtgttg taaagattaa aaaaggcaaa
     1021 ttaggctgtg tatttgtcat aataattggc atatatggta agtgaccaac aaccataagg
     1081 tattataaaa ttgttataaa atgatatgag ctatcattga gcagcatgaa agaagagctt
     1141 cactgtttca cctactatca ccctggccca ttaatctctt tcctgttcct gacatttcag
     1201 agatacgttt aggatttcaa tcatgacctt aagccacatt tgaacaattt tctggtggat
     1261 aagtcctcat tcccacatta tgtatgtacc tagatgcaaa tcctgaatat catgtcgcaa
     1321 ttagtgcatc tggacatgct tgctaactgt gttaaagctc tgaataatgg taaagtttta
     1381 tttctaccaa aacaaatttg ggctgtaatg ttttatgata aaaatctgtg gtcttcctat
     1441 gtacatgtgt gtgtacatgc ttaaaatgca atgttatagt taaatgtaat tcattaaaag
     1501 tatgtaactc cagtggctac ttagtttggc tacttggttt gtagatttct gctttcctgt
     1561 ttcattgtta aacaggtcta gaagttatta tttcatgaaa ctaatgtgag gaaaaagact
     1621 atgttgatat ataagtgaca ttatataaat acatgaggga tgatttgatt agaagcagta
     1681 ttacacagtg ataggagtaa tggtttagaa ctagactcag gtttgaatct tagctctatc
     1741 attataggca tttacttaac ttttcttgtt tgcttaactg aaaactgaag ataataacac
     1801 ctatttacat ggttgttata agggttatat gaataatgtc tggcaaatag taagaactca
     1861 agtaactgtt tcactctttc cagaaggaga ttggctgaaa aatatttgga gtctcctcca
     1921 gccatattcc ttggtcagct tctatgatcc tctttggagc ttaattctta atccctttat
     1981 tttcacttgc ttgttgataa caaagaagaa ctaattatta atttatttca aaatgcatgt
     2041 attatatttg atgggccaca ctaacagtta taaaccaaac aacagattgg gaatggggaa
     2101 gtggatgtgg tgagttcaat cacatgtctg ggaaaagtca atagtgaaga cagagtctca
     2161 caattttttg tcataatgga gagatgaaaa cacaggtaga ggatttcaaa caacagagtg
     2221 gatggtgagt taaaaatgct gaaattcttt cctggtgtct aacttaatgc aatgtggttt
     2281 atctctttgc tcttttctct actattcaaa tttaggataa taaagattaa atgtttctaa
     2341 atcttacttt acaatatcaa gaaaaaaagg tatgcttttg cccacggaag ggcaaagcag
     2401 agctatgaaa acctgctgaa cacattcttt attttcaaca caggttcttg tctttccatc
     2461 atgaaatgca cattttattt gtactgtatt tgggtgacca caagtcaaca acaagataat
     2521 tcacaagacc cttgccttag atgtgtcggc aataaagtaa tcaggccaaa atttttactt
     2581 tcctttgaat ttttcaattc aaacacaatg tatgcttgct tttacacagt agggttcagg
     2641 gattagaggg ttggctcctt taaaaccgtc agagacacag gcaatcctac acaaaattct
     2701 cagaaggaag gcgcctacgc ctgggaatgc ccagatgccc ctcagagagt tgaagatggc
     2761 gtttctctga gtcaggtcaa agttaacaca ttaccttcgc ttcaaagact gcttggcttc
     2821 ctttcggtgg attagtcaag atgttttgct gactgagact aggaaatcta taggagggcg
     2881 ggttagttta cattgttcct tgtcattatc gctaaaacac tccaaagcct tccttaaaaa
     2941 tgcgcactgg gctaaaaagg atagacaagg aacacatcct gggccggtaa ttacgcaaag
     3001 cattatctcc tcttacctcc ttgcagattt ttttttctct ttcagtacgt gtcctaagat
     3061 ttctgtgcca cccttggagt tcactcacct aaacctgaaa ctaataaagc ttggttcttt
     3121 tctccgacac gcaaaggaag cgctaaggta aatgcatcag acccacactg ccgcggaact
     3181 tttcggctct ctaaggctgt attttgatat acgaaaggca cattttcctt cccttttcaa
     3241 aatgcacctt gcaaacgtaa caggaacccg actaggatca tcgggaaaag gaggaggagg
     3301 aggaaggcag gctccgggga agctggtggc agcgggtcct gggtctggcg gaccctgacg
     3361 cgaaggaggg tctaggaagc tctccgggga gccggttctc ccgccggtgg cttcttctgt
     3421 cctccagcgt tgccaactgg acctaaagag aggccgcgac tgtcgcccac ctgcgggatg
     3481 ggcctggtgc tgggcggtca ggacactgac ctggaaggag cgcgcgcgag ggagggaggc
     3541 tgggagtcag aatcgggaaa gggaggtgcg gggcggcgag ggagcgaagg aggagaggag
     3601 gaaggagcgg gaggggtgct ggcgggggtg cgtagtgggt ggagaaagcc gctagagcaa
     3661 atttggggcc ggaccaggca gcactcggct tttaacctgg gcagtgaagg cgggggaaag
     3721 agcaaaagga aggggtggtg tgcggagtag gggtgggtgg ggggaattgg aagcaaatga
     3781 catcacagca ggtcagagaa aaagggttga gcggcaggca cccagagtag taggtctttg
     3841 gcattaggag cttgagccca gacggcccta gcagggaccc cagcgcccga gagaccatgc
     3901 agaggtcgcc tctggaaaag gccagcgttg tctccaaact ttttttcagg tgagaaggtg
     3961 gccaaccgag cttcggaaag acacgtgccc acgaaagagg agggcgtgtg tatgggttgg
     4021 gtttggggta aaggaataag cagtttttaa aaagatgcgc tatcattcat tgttttgaaa
     4081 gaaaatgtgg gtattgtaga ataaaacaga aagcattaag aagagatgga agaatgaact
     4141 gaagctgatt gaatagagag ccacatctac ttgcaactga aaagttagaa tctcaagact
     4201 caagtacgct actatgcact tgttttattt catttttcta agaaactaaa aatacttgtt
     4261 aataagtacc taagtatggt ttattggttt tcccccttca tgccttggac acttgattgt
     4321 cttcttggca catacaggtg ccatgcctgc atatagtaag tgctcagaaa acatttcttg
     4381 actgaattc
//