From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Saccharomyces cerevisiae protein kinase (Ste20p) gene, complete
Date: 3 Jan 93 22:09:41 GMT
Distribution: bionet
Checksum: 48683 8

LOCUS       YSCSTE20P    3809 bp ds-DNA             PLN       21-DEC-1992
DEFINITION  Saccharomyces cerevisiae protein kinase (Ste20p) gene, complete
            cds.
ACCESSION   M94719
KEYWORDS    Ste20p gene; protein kinase.
SOURCE      Saccharomyces cerevisiae DNA.
  ORGANISM  Saccharomyces cerevisiae
            Eukaryota; Fungi; Eumycota; Hemiascomycetes; Endomycetales;
            Saccharomycetaceae.
REFERENCE   1  (bases 1 to 3809)
  AUTHORS   Leberer,E., Dignard,D., Harcus,D., Thomas,D.Y. and Whiteway,M.S.
  TITLE     A protein kinase homologue (Ste20p) required to link the yeast
            pheromone response G-protein B/g subunits to downstream signalling
            components
  JOURNAL   EMBO J. 11, 4815-4824 (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     misc_feature    2074..2106
                     /note="PEST' motif (by similarity); putative"
     misc_feature    2338..3108
                     /note="protein kinase domain (by similarity); putative"
     misc_feature    2425..2427
                     /note="ATP binding site (by similarity); putative"
     misc_feature    2695..2697
                     /note="protein kinase active site (by similarity);
                     putative"
     polyA_signal    3449..3454
     CDS             481..3300
                     /gene="Ste20p"
                     /citation=[1]
                     /note="putative"
                     /product="protein kinase"
                     /codon_start=1
                     /translation="MSNDPSAVSELPDKDSLDNGISNDNERAMGGNGDGGDGLRLPRT
                     TGTLNVNALQKGTNAAHEAGGYKSMDPAKNAETTNDDDNNVVSLDDPIQFTRVSSSSV
                     ISGMSSSMSPHSNIDETKSLEAVTPNINTSNITPDHSADNTFSTINASESDHQFNDTL
                     LSKLSLTDSTETIENNATVKHQQPVASSTVNSNKSSTDIRRATPVSTPVISKPSMTTT
                     PRQINSASHSLSNPKHKQHKPKVKPSKPEAKSKPVSVKKSFPSKNPLKNSSPPKKQTE
                     KSYYSSSSKKRKSGSNSGTLRMKDVFTSFVQNIKRNSQDDKRASSSSNNSSSSSITTA
                     LRISTPYNAKHIHHVGVDSKTGEYTGLPEEWEKLLTSSGISKREQQQNMQAVMDIVKF
                     YQDVTETNGEDKMFKTFNTTTGLPGSPQVSTPPANSFNKFPPSTSDSHNYGSRTGTPM
                     SNHVMSPTLNTDSSSANGKFIPSRPAPKPPSSASASAPIIKSPVMNSAANVSPLKQTH
                     APTTPNRTSPNRSSISRNATLKKEEQPLPPIPPTKSKTSPIISTAHTPQQVAQSPKAP
                     AQETVTTPTSKPAQARSLSKELNEKKREERERRKKQLYAKLNEICSDGDPSTKYANLV
                     KIGQGASGGVYTAYEIGTNVSVAIKQMNLEKQPKKELIINEILVMKGSKHPNIVNFID
                     SYVLKGDLWVIMEYMEGGSLTDVVTHCILTEGQIGAVCRETLSGLEFLHSKGVLHRDI
                     KSDNILLSMEGDIKLTDFGFCAQINELNLKRTTMVGTPYWMAPEVVSRKEYGPKVDIW
                     SLGIMIIEMIEGEPPYLNETPLRALYLIATNGTPKLKEPENLSSSLKKFLDWCLCVEP
                     EDRASATELLHDEYITEIAEANSSLAPLVKLARLKKVAENMDADEDNDDDNDNEHINK
                     TNNCDDNNDSKETVNLDVTEDDKQK"
BASE COUNT     1309 a    842 c    711 g    947 t
ORIGIN      
        1 gaattctatc cagaaccgcc ttctagtagc gtcgctgccc ggctattgcg attctgggac
       61 catattatca gatagctgca ttgtgttaca tgcgatatcg tagaagacga tagaacgaaa
      121 aagaaacgtt aatctttttt tccttttttt ttcgattaaa aaaaaaaaat tttcgcctcg
      181 tggcccgaaa cacggccaag agagggaatt cgaaagtcta ccgcttttgg cagctgaaaa
      241 attcagaaag tcacctggcc agagaggaaa aatacgaaac caaaaagagg catccgtaaa
      301 ttcgcattag caacgcatgc ttacatagat actcacatac tacacacact tacatacttt
      361 cttaaagaca tacatccgta cgtacaatta gagcgaggta gcaagcaacc caaacttctt
      421 cccttcactg cctcacaccc catcctaaat atcccacaag atcctcgact aatacaagaa
      481 atgagcaatg atccatctgc tgtatcggaa ctaccagaca aggacagtct tgataacggt
      541 atcagcaatg acaatgaaag ggccatgggc ggcaatggcg atggcggcga tggattacga
      601 ttaccaagga ccactggaac tttgaacgtc aatgccttac aaaaaggcac taatgctgcc
      661 catgaagctg gtggatacaa atccatggat cctgcgaaga acgcggagac aaccaatgat
      721 gatgacaata atgtcgtttc actagatgat cctattcaat ttacccgagt atcttcctcc
      781 tctgtcatca gtggaatgtc ttcatccatg agtcctcatt ctaacatcga tgaaaccaaa
      841 tctctagaag cagtcactcc aaacataaat accagcaata taaccccgga tcattctgct
      901 gacaacacat tttctaccat aaatgcgtcc gagtcagatc accagtttaa tgacacttta
      961 ctatcaaaac tgtcgttaac agattctaca gaaactatag aaaataacgc gacagtgaag
     1021 caccagcagc cagttgcatc ttccacagta aactcgaata agagctccac tgatatacga
     1081 agggctacac cagtgtccac tcccgttatc tctaaaccat cgatgacaac cacgccaaga
     1141 cagatcaatt cagcttccca ttcgctttcg aaccctaagc ataagcaaca taaaccaaaa
     1201 gttaaaccgt ccaagcctga agcaaaaagt aaaccggttt ctgtgaaaaa aagctttcct
     1261 tcgaaaaatc ctttaaaaaa ctcctctcca cctaaaaagc aaacagaaaa atcgtattat
     1321 tcttcctctt cgaaaaaaag gaaaagcggt tcaaatagtg gtacactaag aatgaaagat
     1381 gtctttacgt cctttgtaca gaatataaag agaaattctc aggatgataa aagggcctca
     1441 tcgtcgtcca ataattcttc ctcatcttct ataaccaccg ctttgaggat atctacgcca
     1501 tacaatgcca agcatatcca ccatgtgggc gtggactcca agactggtga gtacacaggt
     1561 ttgccggagg aatgggaaaa attgttgact tctagtggta tttccaaaag agaacaacag
     1621 caaaacatgc aagcagtcat ggatattgtc aaattctatc aggatgtcac ggaaacaaac
     1681 ggtgaagata aaatgttcaa gactttcaac acaaccacag gattgccggg aagtcctcaa
     1741 gtttcaacac cgcctgcaaa ctcattcaat aaatttcctc cgtcgacaag tgattcgcac
     1801 aattacggtt ccagaacagg tacaccaatg tccaatcacg tcatgtctcc aaccttaaat
     1861 acagattcta gttcagcaaa cgggaaattc ataccaagta gaccggctcc taagccccca
     1921 tcttctgcgt ccgcttcagc tccaattata aaatcacccg tcatgaattc tgccgccaat
     1981 gtttcgccct tgaagcagac tcatgcacct acaactccga acaggaccag cccaaacagg
     2041 tcctcaatat caagaaatgc cactttaaaa aaagaggagc agccactacc accaatacct
     2101 ccaaccaaat ccaaaacgtc tccaatcatc tccacagctc acacaccaca gcaagttgct
     2161 caatcgccaa aagcgccggc gcaagagacg gtaacgacac ctacttcgaa gccagctcaa
     2221 gcaagaagct tgtctaaaga attaaatgag aaaaagagag aggaaaggga aagacgtaaa
     2281 aaacaactat atgccaaatt gaacgaaatt tgctcagacg gtgacccaag tacaaaatat
     2341 gccaatttag taaaaattgg tcaaggtgca tcaggtggtg tttatactgc ttatgaaata
     2401 ggtacgaatg tctcagtggc cattaagcaa atgaatctcg aaaagcaacc aaaaaaggag
     2461 ctaatcatca atgagattct ggtcatgaag ggtagcaaac accctaatat agttaatttc
     2521 attgattctt acgttttaaa aggcgacctt tgggtcatta tggaatacat ggaaggtggc
     2581 tccttaactg atgtggtcac ccattgtatt ttgacagaag gtcaaattgg tgccgtttgt
     2641 agagaaactt tgagtgggtt ggaattttta cattctaaag gtgttcttca cagagatatc
     2701 aaatccgata acatcctatt gtccatggaa ggggatatta agttaacgga tttcggtttt
     2761 tgcgctcaaa tcaatgaatt gaacttgaaa agaactacta tggtgggaac gccttattgg
     2821 atggcgcctg aagtggtttc taggaaagaa tatggcccaa aagtagatat ctggtcgttg
     2881 ggtatcatga tcattgaaat gatcgagggg gagcctccat atttaaatga aaccccgcta
     2941 agagcactgt atttaattgc tacaaatggt acacccaagt taaaggaacc cgagaatcta
     3001 tcgtcaagct tgaaaaaatt ccttgattgg tgtttatgtg tggagcccga agatagagca
     3061 agcgctacgg aattgcttca tgatgaatat atcacggaga tagctgaagc caattcctca
     3121 ttggccccgc tagtcaagtt agcaagattg aagaaagtag ctgagaacat ggatgctgat
     3181 gaagataatg acgacgataa cgacaacgag catattaata agacaaacaa ttgtgacgac
     3241 aataacgata gcaaagaaac cgtaaatttg gacgtaactg aagatgataa acaaaagtaa
     3301 acgtagcaag cagggtacac cttattatcg acaaagtata tacacagttg tgactggcat
     3361 aaaaattctt ttcatatatc ttatcgtgta tatttggaca ttttataaca catcccactc
     3421 taattcacaa cttcattaac gaaatttaaa taaatcacga caacagtttt gcttaaaact
     3481 gaggaatatt gaaaccaact caaattcttc ctaatttcag gcgtataaaa ataacaaatt
     3541 ctcatcgatt gtcgggtacc attacacgaa catctgtctg cgttctatgt aacgaaggag
     3601 aggtattatc caattttgga aatatccgta atattgtcct tagtgcacga actatattat
     3661 cccgcaaatt cagggaaaag aaaagaagta gaaaaaaaaa aataccatgg gagtcagttc
     3721 ttgttcagct gagagaatta cgcttgtttc ttatttccca catatacgag aaattcctac
     3781 cgatataaca tcctctctcg tcttctaga
//