From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: M.kandleri hmd gene for N5,N10-methylenetetrahydromethanopterin
Date: 21 Dec 92 20:33:14 GMT
Distribution: bionet
Checksum: 28389 4

LOCUS       MEPHMDG      1475 bp ds-DNA             BCT       17-NOV-1992
DEFINITION  M.kandleri hmd gene for N5,N10-methylenetetrahydromethanopterin
            dehydrogenase
ACCESSION   X60719
KEYWORDS    N5,N10-Methylenetetrahydromethanopterin dehydrogenase; hmd gene.
SOURCE      Methanopyrus kandleri DNA.
  ORGANISM  Methanopyrus kandleri
            Prokaryotae; Archaeobacteria; Methanogenic bacteria.
REFERENCE   1  (bases 1 to 1475)
  AUTHORS   Zirngibl,C., van Dongen,W.M., Schwoerer,B., Von Buenau,R.,
            Richter,M., Klein,A. and Thauer,R.K.
  TITLE     Hydrogen-forming methylenetetrahydromethanopterin dehydrogenase, a
            novel type of hydrogenase without iron-sulfur clusters in
            methanogen archaea
  JOURNAL   Eur. J. Biochem. 208, 511-520 (1992)
  STANDARD  full automatic
COMMENT     *source: strain=wild-type; *source: clone_library=pUC; *source:
            clone=pMK12;
               From EMBL    entry MKHMDG;  dated 27-OCT-1992.
FEATURES             Location/Qualifiers
     CDS             221..1297
                     /evidence="EXPERIMENTAL"
                     /gene="hmd"
                     /product="N5,
                     N10-methylenetetrahydromethanopterindehydrogenase"
                     /codon_start=1
                     /translation="MVEINKVAILGAGCWRTHAATGITTFKRACEVADETGIKEAALT
                     HSSVTYAVELKHLAGVDEVVLSDPVFDADGFTVVDIEEDCDVDLDEFIKAHLEGNPED
                     VMPKLRDYVNDIADDVPKPPKGAIHFLSPEEMEDKLDIVVTTDDAEAVEDADMIISWL
                     PKGGVQPDIFKKIIDDIPEGCIVANTCTIPTRQFKEMFEDMGRDDLQVTSYHPATVPE
                     HKGQVFVAEGYADEEVVEAVYELGEKARGLAFKVPGYLLGPVCDMASAVTAIVYAGLL
                     TFRDACTDILGAPVDFTQNMAVEALQMMAKFMEEEGLDKLEEALDPAALTNTADSMNF
                     GPLADTEILPKALEVLEKYSKKAE"
BASE COUNT      331 a    428 c    461 g    255 t
ORIGIN      
        1 ggatcctaac tcgcctgtat acccgctcgc ggaagcatga atctcacgac cgacgaaacc
       61 gtgggtgaaa cactccgcgg ttgcgaccct gagcacccaa ggtccccctc atgacgtgta
      121 tttaaccgtt atcccgttgg agcagtcggt gcgctaatta atctcaccac ccggagggat
      181 gacgcattgg taaagatacc ctaacacggg gtgcccacgc atggtcgaga tcaacaaagt
      241 cgcgatttta ggcgccggat gttggagaac ccacgccgcg acgggcatca cgacgttcaa
      301 gcgtgcgtgt gaggtggccg atgagaccgg catcaaagag gccgctctca cgcactccag
      361 cgtgacgtac gccgtcgagc tgaagcacct ggcgggcgtc gacgaggtcg tcctctccga
      421 ccccgtgttc gatgccgatg gcttcacggt cgtggacatc gaagaggact gcgacgtgga
      481 tctggacgag ttcatcaagg cgcacctcga gggtaacccc gaggacgtga tgccgaagct
      541 acgggactac gtgaacgaca tcgccgatga cgtcccgaag ccgcccaagg gagccatcca
      601 cttcctgagc cccgaggaga tggaggacaa gctcgacatc gtcgtgacca cagacgacgc
      661 ggaggccgtc gaggacgcgg acatgatcat ctcctggttg ccgaaaggcg gagttcagcc
      721 tgatattttc aagaagatca tcgacgacat cccggaaggc tgcatcgtag ccaacacctg
      781 tacgatcccg acgcgacagt tcaaggagat gttcgaagac atgggccgcg atgatctgca
      841 ggtaacctcc taccacccgg cgaccgtgcc ggagcacaag ggtcaggtgt tcgtggcaga
      901 aggttacgcg gacgaggaag ttgtcgaggc cgtctacgag ctgggtgaga aggcccgcgg
      961 actggcgttc aaggtcccgg gctacctgct gggcccggtg tgcgacatgg ccagcgccgt
     1021 cacggcgatc gtgtacgccg gcctgctgac gttccgagat gcgtgtaccg acatcctagg
     1081 agctcccgtg gacttcaccc agaacatggc cgtagaagca ctgcagatga tggcgaagtt
     1141 catggaggaa gagggtctag acaagctaga ggaggccctg gacccggcgg cgctcacgaa
     1201 cacggccgac tcgatgaact tcggcccgct ggcggacacg gagatcctgc cgaaggccct
     1261 agaggtcctg gagaagtact cgaagaaggc ggagtaagcc cctttacttt cccccacagt
     1321 ctctttccag gggagctcgt tgaaggaacc ggaaccggga ataaccgatc tcacggagac
     1381 cgtccggtca cgtccgttct gggacgtcgt caaggagatc gggttagcta aagccgagga
     1441 aatcgctgaa gttgttccgg aaggttcccg gatcc
//