From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: D.pseudoobscura Adh gene for alcohol dehydrogenase
Date: 28 Dec 92 21:26:42 GMT
Distribution: bionet
Checksum: 57005 8

LOCUS       DROADHGN21   3479 bp ds-DNA             INV       01-DEC-1992
DEFINITION  D.pseudoobscura Adh gene for alcohol dehydrogenase
ACCESSION   X62201
KEYWORDS    ADH gene; alcohol dehydrogenase.
SOURCE      Drosophila pseudoobscura DNA.
  ORGANISM  Drosophila pseudoobscura
            Eukaryota; Animalia; Metazoa; Arthropoda; Uniramia; Insecta;
            Pterygota; Neoptera; Holometabola; Diptera; Brachycera;
            Cyclorrhapha; Schizophora; Drosophiloidea; Drosophilidae.
REFERENCE   1  (bases 1 to 3479)
  AUTHORS   Schaeffer,S.W. and Miller,E.L.
  TITLE     Estimates of gene flow in Drosophila pseudoobscura determined from
            nucleotide sequence analysis of the alcohol dehydrogenase region
  JOURNAL   Genetics 132, 471-480 (1992)
  STANDARD  full automatic
COMMENT     *source: strain=GB24; *source: isolate=Gundlach Bundschu Winery,
            Sonoma Valley, *source: California; *source: chromosome=IV section
            88;
               From EMBL    entry DPADHGN21;  dated 16-OCT-1992.
FEATURES             Location/Qualifiers
     mRNA            join(40..119,921..1026,1090..1494,1555..1972)
                     /note="adult mRNA (Adh)"
     mRNA            join(888..1026,1090..1494,1555..1972)
                     /note="larval mRNA (Adh)"
     promoter        8..14
                     /note="adult promoter (Adh)"
     prim_transcript 40..1972
                     /note="adult primary transcript (Adh)"
     exon            40..119
                     /note="adult exon 1a"
     intron          120..920
                     /note="adult intron"
     promoter        852..859
                     /note="larval promoter (Adh)"
     prim_transcript 888..1972
                     /note="larval primary transcript (Adh)"
     exon            888..1026
                     /note="larval exon 1"
     exon            921..1026
                     /note="adult exon 1b"
     intron          1027..1089
                     /number=1
     exon            1090..1494
                     /number=2
     intron          1495..1554
                     /number=2
     exon            1555..1972
                     /number=3
     polyA_signal    1949..1954
     polyA_site      1972
     CDS             join(934..1026,1090..1494,1555..1821)
                     /EC_number="1.1.1.1"
                     /gene="Adh"
                     /product="alcoholdehydrogenase"
                     /codon_start=1
                     /translation="MSLTNKNVVFVAGLGGIGLDTSRELVKRNLKNLVILDRIDNPAA
                     IAELKAINPKVTITFYPYDVTVPVAETTKLLKTIFAQVKTIDVLINGAGILDDHQIER
                     TIAVNYTGLVNTTTAILDFWDKRKGGPGGIICNIGSVTGFNAIYQVPVYSGSKAAVVN
                     FTSSLAKLAPITGVTAYTVNPGITKTTLVHKFNSWLDVEPRVAEKLLEHPTQTSQQCA
                     ENFVKAIELNKNGAIWKLDLGTLEPITWTQHWDSGI"
BASE COUNT      975 a    791 c    797 g    916 t
ORIGIN      
        1 cgtagtgtat ttaaggcact gcacatgtcg aggaccacga ttattgtcta cagtgcagtc
       61 agtagttgtc aggtgcagca ggcggaccag catttcttta aatctactca atcgatcaag
      121 taagtaactg ttatccaagt acagtcggcc catcccactc ggtcactcgg ttctgcagta
      181 actcaagaaa tttcttgata gcgcagttcc agttgtaaaa cgtcgtttaa aaaaacatct
      241 cgttggagct tgggttgaaa aacgttcgag ttgggcgtag aaaagttcaa atataaaata
      301 tcaaaaaaga gaaaatatct tcccaaagca gctgtccatt ttttgttaaa gtgatagatc
      361 aaaaattcgt atgacgtaca ttcctagtgc acttcataca aataatttcc tcaattctaa
      421 ttctacaaga ggcacttttg gctcttgtcc agaaaaaaca atttgtttac acttgtcgga
      481 tgataacaga agcggaccga cggctaagta acataacagt cgcctgttaa gtaatgccct
      541 cactctctgt tagatggccg cgcgtgtcga ctccgataga ccagcagaca cgcagtagtt
      601 ttcctcaaga acctgaactc taaacataga cataatttac gataccttga gactcaaata
      661 aaaatacaag tatgagtggt attctattcc tgattagact cgcacacaca catacgagta
      721 ttaacataaa tttgccggca aataaccaaa acaaaacgaa cgcaaacgat ccgaacacgt
      781 aatgcgagag ataagaaaca gaaaagctcg acgtgaaaga agcttctggc ggaagctctt
      841 gccgaactcc ctataaatac gagacagaaa accagaataa atataataac aaactgttgt
      901 attgtcccat cgattcccag atcaaaagac agaatgtctc tcaccaacaa gaacgtcgtt
      961 ttcgtggccg gtctgggcgg cattggcctg gacaccagcc gggagttggt caagcgtaat
     1021 ctgaaggtaa gagagaaaga attccatgga atctatggaa tcctaaattt aaaaatttat
     1081 attttttaga acctggtcat cctggatcgc attgacaatc cggctgccat tgccgaactg
     1141 aaggcaatca atcccaaggt gaccatcacc ttctatccct acgatgtgac tgtgcccgtc
     1201 gctgagacca ccaagctcct gaagaccatc tttgcccagg tgaagaccat cgatgtcctg
     1261 atcaacggtg ctggcatcct ggacgatcat cagatcgagc gcaccattgc cgttaactac
     1321 acgggcctgg tcaacaccac cacagccatt ctggacttct gggacaagcg caagggcggc
     1381 ccaggcggca tcatttgcaa cattggctcc gtcaccggtt tcaatgccat ctaccaggtg
     1441 cccgtttact ccggctccaa ggcggcggtg gttaacttta cctcctccct ggcggtaagt
     1501 aatcctacaa ctatcctact ccccaaaact aaatatttct ttgaaatctc atagaaactg
     1561 gctcccatta ctggtgtcac tgcttacact gtcaatcctg gcatcactaa gaccactctg
     1621 gtccacaaat tcaactcgtg gctggacgtg gagccccgtg tggcggagaa gctgctcgag
     1681 catcccaccc agacctctca gcagtgcgcc gagaactttg tgaaggccat tgagctgaac
     1741 aagaacggtg ccatctggaa gttggatttg ggcaccttgg agcccatcac atggacccag
     1801 cactgggact cgggcatcta aagatcatcc cagagactct atgggacatg gcgttttagc
     1861 ttttagtttc tttttttcca ctcaattgtt acgtatatac atacacatat ggcaataagg
     1921 ctgatttgaa ccccgttttg aatatgataa tacaaaatta tatttgagaa atttcaacaa
     1981 aatcgataag agctatagct ctcttttaat taaaaaaaac tagataaaaa gagcaatggt
     2041 cagtggtggt cagtggtcgt tgtggcctct atcgatttcc acacaaaaac tttactgttt
     2101 agtaatagaa aagaacttga gaaaggcagc caaaagaatg tacgatctga cgggtaagca
     2161 tgtctgctat gtggctgact gcggtggcat tgcactggag accagcaagg ttctcatgac
     2221 caagaatata gcggtgagtg gtagagtggt gtagagagtg gagagtggtt ctggttctta
     2281 aacaagtctc tagtctctag tctcttggct gctgctggta gctgtcgaaa ctaattaagt
     2341 taatgacttt ttcatgtttt gtttttgtcc gctgactttt gctgttcaaa gctacaatta
     2401 agtttatgtt ttgagtttga tctacttttg cttaagggaa gtactttcga ttacggatcg
     2461 aatggctggc atataaaaat agataccaag gaatgtgcct catatcatat tgattctatc
     2521 ttgattcttt gcctagaaac tggccatcct tcagagcgtg gaaaaccccc cggccatcgc
     2581 tcagttacaa tcgattaagc acagcacaca gatcttcttc tggaccttcg atgtgaccat
     2641 ggcccgcgag gagatgaaga agtacttcga tgaggtcatg gtccagatgg actacatcga
     2701 tgtgctgatc aatggggcca ccctgtgcga tgagcggaac attgatgcca ccatcaatac
     2761 gaatctgacc ggcatgatga acaccgtggc cactgtgctg ccctatatgg accgtaagat
     2821 gggcggttcg ggtggactga ttgtgaatgt cacctccgtc ataggactgg atccatcgcc
     2881 agtcttttgt gcgtacagtg cctcaaagtt tggtgtgatt gggttcaccc gaagtctagc
     2941 ggtgagtggg cgagatctta gcttgaaaat gctctctaat gtctttcatt ctttcgcagg
     3001 atcccctgta ttacacccaa aatggtgtgg ctgtcatggc cgtctgctgt ggtcccacca
     3061 aagtgtttgt cgatcgggaa ctgactgcct tcctggcata cggccaaacc tttgccgatc
     3121 gcctgcgtcg tgctccctgc caatcgacgg ccgtctgcgg ccagaatata gtaaatgcca
     3181 tcgagagatc ggagaacgga cagatctgga ttgccgacaa gggcggcctg gagtcggtgg
     3241 cccttcactg gtattggcat atggccgatc agtttgtgaa ctacatgcag agcactgacg
     3301 atgaggatca ggagtttttt ttgggccagc gatgaggcat gccttgatcc acggaataaa
     3361 acatcaaatg tgtcacaaaa agtaggcaac ttgtttgtgg gtctggaaat agatttgaaa
     3421 tattttgagg tcatatgaat aatgacataa atgagggtgc ctgaaagtat gccaagttt
//