From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Pseudanabaena sp. phycocyanin-1 beta subunit (cpcB1); phycocyanin-1
Date: 20 Aug 92 10:04:24 GMT
Distribution: bionet
Checksum: 40965     9

LOCUS       PUNCPCBI     3689 bp ds-DNA             BCT       20-AUG-1992
DEFINITION  Pseudanabaena sp. phycocyanin-1 beta subunit (cpcB1); phycocyanin-1
            alpha subunit (cpcA1); and phycocyanobilin lyase (cpcE1) genes,
            complete cds's.
ACCESSION   M99426
KEYWORDS    phycocyanin-1 alpha subunit; phycocyanin-1 beta subunit;
            phycocyanobilin lyase.
SOURCE      Pseudanabaena sp. (library: PCC 7409) DNA.
  ORGANISM  Pseudanabaena sp.
            Prokaryotae; Gracilicutes; Oxyphotobacteria; Cyanobacteria;
            Nostocales; Oscillatoriaceae.
REFERENCE   1  (bases 1 to 3689)
  AUTHORS   Dubbs,J.M. and Bryant,D.A.
  TITLE     Organization and transcription of the genes encoding two
            differentially expressed phycocyanins in the cyanobacterium
            Pseudanabaena sp. PCC 7409
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     RBS             478..482
     CDS             493..1011
                     /function="light-harvesting protein found in peripheral
                     rods of phycobilisomes"
                     /product="phycocyanin-1 beta subunit"
                     /gene="cpcB1"
                     /codon_start=1
                     /translation="MYDAFAKVVSQADSRGAYISAAQIDALNAMVADGSKRLDAVNRI
                     TSNSSAIVANAARALFAEQPALIAPGGNAYTSRRMAACLRDMEIVLRYVTYAIYSGDA
                     SILEDRCLNGLRETYLALGTPGSSVAVGIGKMKDAAIAIANDPNGVTRGDCSALMSEI
                     GSYFDKAAAAVA"
     RBS             1149..1154
     CDS             1164..1652
                     /function="light-harvesting protein found in the
                     peripheral rods of phycobilisomes"
                     /product="phycocyanin-1 alpha subunit"
                     /gene="cpcA1"
                     /codon_start=1
                     /translation="MKTPLTEAVSAADSQGRFLSSTAVQVAFGRFRQATSGLAAAKAL
                     SEKADSLASGARNAVYSKFPYTTSMTGANYASTQTGKDKCVRDIGYYIRMVTYCCVVG
                     GTGPMDDYLVAGIAEINRTFDLSPSWYVEALKYVKANHGLSGDSAVEANSYIDYAINA
                     LS"
     stem_loop       1664..1698
     stem_loop       1706..1741
     RBS             1749..1754
     CDS             1768..2583
                     /function="attachment of phycocyanobilin to the
                     phycocyanin alpha subunit apoprotein"
                     /product="subunit of phycocyanin alpha subunit
                     phycocyanobilin lyase"
                     /product="phycocyanobilin lyase"
                     /gene="cpcE1"
                     /codon_start=1
                     /translation="MNEETGIPDDNLLTIEQAIANLQSEDLGLRVYAAWWLGRFRVNV
                     PEAIDLLIAALADEADRTEAGGYPLRRNAARALGKLGDRRAVPALITSLECDDFYVRE
                     AVAQSLEMLGDNSCVPYLIELIKNPNPEASADTEPEHPYDAFLEALGTLGAVKAIPHI
                     LPFLDHPIPKVQYAAARAMYQLSEPMVADQYGDRLVAALANDDLQLRRTVLSDLGAIG
                     YLSAAEAIGNTMAENSLKLISLKGLLEKQIVLATPPDLTDGAIRVMALMDDLL"
     stem_loop       2599..2659
     RBS             2704..2710
     RBS             2714..2719
     CDS             2738..3586
                     /function="attachment of phycocyanobilin to the
                     phycocyanin alpha subunit apoprotein"
                     /product="subunit of the phycocyanin alpha subunit
                     phycocyanobilin lyase"
                     /product="phycocyanobilin lyase"
                     /gene="cpcF1"
                     /codon_start=1
                     /translation="MTDRLSQLIQAVEEADSSKLLLEAVQNLAAARLEGAIPILIEAL
                     SYNNPGAAVASVDGLILLGSAAVPPLLELLDMHNYTARAWAIRALAGIGDPRGLVTLL
                     GVATADFAMSVRRAAVKGLGTMKWHWFPDELREIAQEEALDALLFVAQQDEEWVVRYA
                     SVVGLQSLAIAIAEAHPTWLAEIQTQFNLMSCNDEVLAIRGRVCVAQQKLQQKIQQLK
                     SDSFHKSIEIKETEEKKSPLTSNDWQDILNQLYEIKRQERINSGSEGDPRRFQQLAAA
                     ITETNN"
     stem_loop       3589..3623
BASE COUNT     1050 a    770 c    816 g   1053 t
ORIGIN
        1 tagatataca aaaaatatgt tttttgattg aaaatctgct cttgcgtcta ctctatgcag
       61 atccagcaat tgttgctgaa aatacagcaa attttgaaac aagaacgaaa aaggtaatgc
      121 tgtcatagct agaaacgcct ttttccgtgg gatcaatggc gctccgtagc ccaaaaccgt
      181 gatcccgaaa caagccgtta taaattgtga caatctgtaa taaaaaatga tccaacgaca
      241 ttgtataaac aaataggtta gaaattaaac caatgaaata aatgaacagc agtcatttgc
      301 ctatcattta ttacgtctag gttaataaac tagctattta taaaaacaaa gtttttctaa
      361 atttctcgaa agagaaaatc agaaacaatt aagatagctg ctaacaatta aagaactgca
      421 atagttagtt tcagattgtt aactgtctaa aaaataaaag ataattactc aataactgga
      481 gataacagta ttatgtacga cgcatttgca aaggtagtat cccaagctga ttccagaggc
      541 gcttatatca gtgcggcgca aattgatgct cttaacgcaa tggttgctga tggcagcaag
      601 cgtttggacg ctgtaaaccg catcaccagc aactcttctg caatcgtagc taatgctgct
      661 cgtgctttgt tcgctgaaca gcctgctttg atcgctcctg gtggaaatgc atacaccagc
      721 cgtcgtatgg cagcttgctt gcgcgacatg gaaatcgttt tgcgctatgt tacctacgct
      781 atctactctg gcgatgcaag catcttggaa gatcgttgcc tcaatggttt gagagaaacc
      841 tatttggctc ttggtactcc tggttcttcc gttgctgtag gtatcggcaa gatgaaggat
      901 gcagcgatcg caatcgctaa cgatcctaac ggcgtaaccc gtggcgattg ttccgctctt
      961 atgagtgaaa tcggtagcta cttcgacaag gctgctgctg ctgttgctta gtttttagtt
     1021 ccttagtctg ggcttcgtat tgactggact aaagactgaa taatttaatt ttttgatttc
     1081 attccattgg ttctgactga attaatgaat ttttcatcag atcgcattta ccttaacaag
     1141 attattttgg agaacatctc acaatgaaaa cccctttaac cgaagcagta tccgccgccg
     1201 attctcaagg tcgtttcctc agctctacag ccgttcaagt tgcttttggt cgtttccgtc
     1261 aagctacctc tggtctagct gctgctaagg ctttgagcga aaaggctgat tctttggctt
     1321 ctggcgcacg taacgctgta tacagcaagt tcccttacac cacctccatg actggtgcta
     1381 actacgcttc tacccaaact ggtaaagaca agtgcgtacg tgacatcggc tactacatcc
     1441 gtatggttac atactgttgt gtagttggtg gtacaggtcc aatggatgat tacttggttg
     1501 ctggtattgc agaaatcaac cgcacctttg atctttctcc tagctggtac gttgaagctc
     1561 tcaagtatgt taaagctaac cacggtttgt ctggagactc tgctgttgaa gcaaactcct
     1621 acatcgacta tgcaatcaat gcacttagct aattttttct aagaaacaaa aagcacccca
     1681 atggggtgct ttttgttttc aagccaagca taaaaggcgg aaagcgctgc cttttatgct
     1741 tatgtagtaa aaggtcacat taaaactatg aacgaagaaa ctggtattcc cgacgataat
     1801 ttattaacca ttgaacaggc gatcgcgaat ctccaaagtg aagacttagg attacgagtt
     1861 tatgcggctt ggtggttggg gcggtttcgg gtgaatgtac cagaagcgat cgatctttta
     1921 attgcggctt tagccgacga agccgatcgc actgaggctg ggggctatcc attacgacgc
     1981 aatgcagcga gagcgttagg gaaattaggc gaccgccgtg ctgtacctgc attaatcaca
     2041 tctttggaat gcgatgactt ttatgtacgt gaagctgtgg ctcaatcgtt agaaatgttg
     2101 ggcgataatt cctgcgtacc ttacttaata gagttaatca aaaatcctaa ccctgaagct
     2161 agcgccgaca cagaaccaga acatccttac gacgcatttc tcgaagcatt gggaactcta
     2221 ggtgcagtca aagcgattcc tcacattttg ccgtttttag atcatccgat tcctaaagta
     2281 caatatgcgg cggctagagc aatgtaccaa ctttcggagc ctatggttgc cgatcaatat
     2341 ggcgatcgct tagttgcggc tcttgctaat gatgacttgc aactacggcg tacagttctt
     2401 tcggacttgg gggcgatcgg ttatttatcg gcagcagaag cgatcggtaa tacgatggca
     2461 gagaatagtc ttaagttaat ttcacttaaa ggtttgttag aaaaacaaat agttctggcg
     2521 actccaccag atttgactga tggcgcaatt agagtcatgg cattgatgga tgatttgtta
     2581 tagctacagt cacttgctat aaaaaaccaa gaatcgattg cgagcgatct gcgctgctaa
     2641 ttgattcttg gttttttatg tcctagtaaa gttttggtac tgatggatga tttattgtag
     2701 agtagaggag cgcaaagagc ttctctaacc tatctatatg actgatcgcc tttcccaact
     2761 tatccaagct gtagaagaag cagattcttc caaactattg ctagaagcag tccaaaatct
     2821 ggcggcggct cgtttagaag gtgctattcc catattgatt gaggctctta gttataacaa
     2881 tccaggagcc gcagttgcat ctgtggatgg attaatttta ctgggaagtg cggctgtacc
     2941 gcctttgctc gaacttctag atatgcacaa ttacacagca cgcgcatggg caattcgagc
     3001 cttagcaggg attggtgatc caagaggttt agtaacttta ctaggggtag caactgcgga
     3061 ctttgcgatg agtgtgcgcc gcgccgccgt taagggttta ggtacaatga aatggcattg
     3121 gtttcctgat gaattaaggg aaatcgccca agaagaagcc ctagatgcct tactctttgt
     3181 agcacagcag gatgaagaat gggtggtgcg ctatgcctca gtagtgggct tgcagtcttt
     3241 agcgatcgcg atcgctgaag cccatcctac ttggctagcg gaaattcaaa cgcaattcaa
     3301 tttgatgtct tgcaatgatg aagttctcgc cattcggggt cgggtttgcg tagcgcaaca
     3361 gaagcttcag caaaaaattc aacaacttaa atctgatagc tttcataagt ccatagaaat
     3421 aaaagagact gaagagaaaa aatcaccact tacttctaat gattggcaag atattctcaa
     3481 tcaactttat gaaattaagc gacaagagcg cattaattct ggcagcgaag gtgacccacg
     3541 tcggtttcag caattagcag cagcaatcac cgagacaaat aattaataca aaacacaaaa
     3601 tggctacacc attttgtgtt ttggtataac ggcagtaatc ttcaaggaat ttcctcacgc
     3661 tctaaaccct aactccatta aagccttac
//