HDTenEightyP/NetNews huggingface dataset:
From: adavies@vax.oxford.ac.uk (Anthony Davies)
Newsgroups: bionet.molbio.methds-reagnts
Subject: Re: Baculovirus expression question(s)
Date: 12 Nov 92 10:01:28 GMT
Organization: Institute of Virology, Oxford

In article <10NOV92.11550841@wums.wustl.edu>, wetsel_r@wums.wustl.edu writes:
> 1) are spinner bottles really necessary for production of recombinant virus.
No, but they're more convenient than flats for scale up.  Use whatever you 
like - insect cells are basically indestructible.
> 2) When would one choose to use a fused versus non-fusion vector and why?
Fusion=affinity tagging in this case I assume.  So it wouldn't be required 
if, for instance, you already have an antibody to your protein and like to 
purify it that way.  Also the tag itself does add extraneous residues to your
sequence.
  3)  The InVitrogen manual suggests using a surfactant, Pluronic F-68 so that 
the sf9 cells aren't damaged by shear forces - has anyone found this to be 
necessary?
Absolutely not

Good luck, and mail me with any other requests - Anthony.
________________________________________________________________________________

Anthony Davies                Voice: 865-512361
Institute of Virology         Fax:   865-59962
Mansfield Road                Vax:   adavies@vax.path.ox.uk.ac (preferred)
Oxford OX1 3SR                       adavies@vax.ox.uk.ac (this account)
UK
________________________________________________________________________________
From: MCAO@VTHVAX.TAMU.EDU
Newsgroups: bionet.molbio.methds-reagnts
Subject: What causes the plaque size difference?
Date: 11 Nov 92 15:06:55 GMT
Distribution: bionet

Hi, Dear netters: I raised a question two days ago about the "phage size". I am
sorry I did not make it clear. Here phage size I mean plaque size. Each time
when I grow the phage bearing the cDNA library, the size of the plaque varies.
					Ming Cao
From: bfrank@aardvark.ucs.uoknor.edu (FRANK,BART)
Newsgroups: bionet.molbio.methds-reagnts
Subject: anti-GST antibodies for fusion protein detection
Date: 12 Nov 92 21:11:00 GMT
Organization: University of Oklahoma - University Computing Services
Originator: usenet@midway.ecn.uoknor.edu
News-Software: VAX/VMS VNEWS 1.41

We are making GST fusion proteins and it appears that only small
amounts of the fusion protein is produced or survives protelysis.
Can anyone recommend a source for an anti-GST antibody that
should recognize the GST moeities in Western blots?

Thanks,
Bart Frank
Internet: BFRANK@AARDVARK.UCS.UOKNOR.EDU
From: IBELGAUFTS@vms.biochem.mpg.de
Newsgroups: bionet.molbio.methds-reagnts
Subject: cDNA cloning artifact help or comment
Date: 6 Nov 92 09:27:19 GMT
Distribution: bionet
Original-To: methods@uk.ac.daresbury

dear colleagues,
I have isolated a cDNA clone from a brain cDNA library.
This clone contains a 3 kb ORF which perfectly matches a known
gene which we are investigating. At one end of the ORF there is a
short 150 bp fragment which does not have an ORF and is not related
to the 3 kb sequence. A search of databases shows that these 15o bp
are not homologous to any known sequence.
Does anyone out there have an explanation for this or could point me
to literature describing similar observations?
***********************************************************
Horst Ibelgaufts
Institut fuer Biochemie der LMU Muenchen
im Max Planck Institut fuer Biochemie, Raum F310
Am Klopferspitz 18a
D 8033 Martinsried
FRG
***********************************************************
thank you
From: afc@gnv.ifas.ufl.edu
Newsgroups: bionet.molbio.methds-reagnts
Subject: Re: Cause of phage difference?
Date: 12 Nov 92 19:36:40 GMT
Distribution: bionet

In article <921110102536.2f74@VTHVAX.TAMU.EDU>, MCAO@VTHVAX.TAMU.EDU writes:
> 
> 	Hi, the size of the phage on my screening plate varies a lot. What may
> cause this phenomenon? The insert size or the bacterial strain?
> 						Ming Cao

You don't say whether this is within a single plate or between plates.

On a single plate there are often differences between individual plaques.
These can be due to different inserts, to poorly poured plates (thicker
on one side than the other), and to differences in where in the agar the
plaque started (remember that a plaque is a section of a sphere, not a circle).

Between plates differences can be due to amount of moisture in plate, amount
of top agar, amount of cells, and age of cells.

This is only a partial list of possible causes.  Just about anything can
affect plaque size.

Andrew Cockburn
USDA
From: 76467.702@CompuServe.COM (Alan Gerstein)
Newsgroups: bionet.molbio.methds-reagnts
Subject: Re: Ligation techniques
Date: 9 Nov 92 16:52:11 GMT
Distribution: bionet

G'day,

You might want to check out Hinton et al, NUC. Acid Res. 10, 1877 (1982), & 
Harrison and Zimmerman, Nuc. Acid Res. 12, 8235 (1984).

Alan

Distribution:
  INTERNET:phjf@ariel.ucs.unimelb.EDU.AU INTERNET:phjf@ariel.ucs.unimelb.EDU.AU
  METHODS-AND-REAGENTS >INTERNET:methods@net.bio.net
From: 76467.702@CompuServe.COM (Alan Gerstein)
Newsgroups: bionet.molbio.methds-reagnts
Subject: Anti GST antibody
Date: 13 Nov 92 21:37:21 GMT
Distribution: bionet

AMRAD (Australia) currently sells anti GST antibody.  Their fax number is 63 
03 853 0202.

In the U.S., The Binding Site, San Diego and Oncor, Bethesda, Md. sell it, but
the antibody is raised against a GST from a different species than that in the
pGEX vector.

Pharmacia will distribute the AMRAD antibody in the near future.
From: bioc07@otago.ac.nz
Newsgroups: bionet.molbio.methds-reagnts
Subject: Re: Aqua Bidest?
Date: 6 Nov 92 09:05:08 +1300
Distribution: bionet
Organization: University of Otago, Dunedin, New Zealand

In article <921104115844.20206443@ntet.nctr.fda.gov>, WMELCHIOR@NTET.NCTR.FDA.GOV (Bill Melchior, NCTR/FDA) writes:
> A researcher here has an HPLC procedure from a German paper that calls for 
> "Aqua Bidest" as part of the solvent system.  We've so far been unable to 
> determine what this is -- can anyone help us?
> 
> Thanks
> ________________________________________________________________________________
> The opinions stated are mine, not those of NCTR or its sponsoring organizations.
> 
> Bill Melchior                                ||     OMNISCIENCE
> National Center for Toxicological Research   ||    Knowing what
> Jefferson, AR  72079                         ||    thou knowest not
> (501) 543-7206                               ||    is in a sense
>                                              ||    omniscience.
> WMELCHIOR@NTET.NCTR.FDA.GOV                  ||       from Grooks, Piet Hein


How about double distilled water?

     %%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%
     %  Craig Marshall                               %
     %  ++++++++++++++++++++++++++++++++++++++++++++ %
     %  Biochemistry Department bioc07@otago.ac.nz   %
     %  University of Otago     Phone 64 3 479 7849  %	
     %  P.O. Box 56             Fax   64 3 479 7866  %	
     %  Dunedin                                      %
     %  New Zealand                                  %	
     %%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%
From: ca566@cleveland.Freenet.Edu (Michael Holloway)
Newsgroups: bionet.molbio.methds-reagnts
Subject: In vitro mutagenesis kit recommendation?
Date: 11 Nov 1992 15:35:55 GMT
Organization: Case Western Reserve University, Cleveland, OH (USA)
Reply-To: ca566@cleveland.Freenet.Edu (Michael Holloway)
NNTP-Posting-Host: hela.ins.cwru.edu


I apoligize if I'm lowering the intellectual level of this conference :-)
but I promised to post an inquiry to see if there are any favorite kits for
mutagenesis.  Is there any difference in the products?  Is there a
consensus on a favorite?

Thanks
Mike
 
-- 
E-mail: mhollowa@ccmail.sunysb.edu (mail to freenet is forwarded)
phone:  (516)444-3612
From: bsh@MED.PITT.EDU (Basavaraju Shankarappa)
Newsgroups: bionet.molbio.methds-reagnts
Subject: Re:  Western Blot staining
Date: 12 Nov 92 15:44:23 GMT
Distribution: bionet

The reagent that can be used to stain the blotted membranes is
ponceau S at 0.5% in DW, But dissolve in glacial acetic acid first.
Destain in DW.
Raj Shankarapp
bsh@med.pitt.edu
From: brknig01@uctvax.uct.ac.za
Newsgroups: bionet.molbio.methds-reagnts
Subject: Address wanted: Dr Igloi
Summary: Igloi, rpoc2, RNApol
Keywords: rpoc2, RNA polymerase, Igloi
Date: 12 Nov 92 13:12:53 GMT
Organization: University of Cape Town

Hi  netters... I am looking for Dr Igloi's email address. He is (or was)
working on chloroplast RNA polymerase genes. If anyone knows his address or
whereabouts please let me know
From: robert@arbo.microbiol.uwa.oz.au (Robert Coelen)
Newsgroups: bionet.molbio.methds-reagnts
Subject: Vent of Pfu - which is more HiFi ?
Date: 6 Nov 92 05:34:06 GMT
Organization: University of Western Australia
NNTP-Posting-Host: mve.microbiol.uwa.edu.au


Hi,

Has anyone out there compared the thermostable polymerases Vent and Pfu ?
Any comments you may have on the RELATIVE merits of one over the other would
be appreciated (eg length of synthesis products, stability, etc)


Thanks for your efforts


Robert Coelen
Dept of Microbiology
University of western Australia
From: WAGNERF@BRUNB.BITNET (Wagner Fontes)
Newsgroups: bionet.molbio.methds-reagnts
Subject: RE: AQUA
Date: 7 Nov 92 00:56:57 GMT
Distribution: bionet


  HI BILL !

    That means double destilled water.

Wagner Fontes
Brazilian Center of Protein Sequencing
Biochemistry and Protein Chemistry Lab.
University of Brasilia - Brazil
EMAIL WAGNERF@BRUNB




.
From: wetsel_r@wums.wustl.edu
Newsgroups: bionet.molbio.methds-reagnts
Subject: Baculovirus expression question(s)
Date: 10 Nov 92 11:55:08 GMT
Organization: MIT PLASMA FUSION CENTER
NNTP-Posting-Host: msnews.wustl.edu

Greetings netters:

We are attempting to insert our favorite cDNAs into a baculovirus
expression system.  We purchased InVitrogens.  We are a bit new to this
system and we have a couple of questions:  1) are spinner bottles really
necessary for production of recombinant virus.  2) When would one choose to
use a fused versus non-fusion vector and why?  3)  The InVitrogen manual
suggests using a surfactant, Pluronic F-68 so that the sf9 cells aren't
damaged by shear forces - has anyone found this to be necessary?

Any helpful hints beyond these questions would be appreciated...

thanks in advance,

David Haviland
haviland@kids.wustl.edu
From: sandy@pt.Cyanamid.COM (Sandy Silverman)
Newsgroups: bionet.molbio.methds-reagnts
Subject: Western Blot staining
Date: 11 Nov 92 21:54:26 GMT
Distribution: bionet

We once read about a method to detect protein on a Western blot prior
to antibody detection.  It involved a red dye and acetic acid.  It was 
reversible.  Does anyone have the reference?   Thanks-----Sandy
From: wsun@jeeves.ucsd.edu (Fiberman)
Newsgroups: bionet.molbio.methds-reagnts
Subject: stable transfection
Date: 8 Nov 92 22:30:41 GMT
Organization: University of California, San Diego

Hello Netter,

I am going to use the pRc/CMV vector from Invitrogen to express a cDNA
in HELA cells.  I want to select for the transfected cells with
neomycin resistance and establish a stably transfected cell line
expressing my clone.  Can anyone out there recommend a good reference,
or give some helpful tips on how to proceed?  Any suggestions
would be appreciated.

-fm

Newsgroups: bionet.molbio.methds-reagnts
From: bchs1b@Elroy.UH.EDU (Michael Benedik)
Subject: Re: sequencing from 300-500 bp
Reply-To: bchs1b@Elroy.UH.EDU
Organization: University of Houston
Date: Thu, 12 Nov 1992 06:19:33 GMT

In article <Af0KlOu00iV005CmZv@andrew.cmu.edu>, "Morris F. Manolson" <mm6y+@andrew.cmu.edu> writes:
>Dear Netters,  I am sure someone must have asked this before but......
>
>I am trying to sequence off of one reaction 300 to 500 bps using the 
>standard IBI or BRL 44 cm glates.  Without having to resort to ultra-long
>plates (of which I don't have), does anyone have any suggesting on how to
>improve the resolution of the bands in the 300 to 500 bp region.  My reactions
>are fine and I can see bands up to 600 bp from the primer, I just can't
>resolve them well enough to read.  I am using 6% acrylamide wedge gels and run
>them for 24 hours at 900 Volts, this gives me nice sequence on average
>up to 350 bp,  on a great gel I can get 400.  I remember some company trying
>to sell an alternative matrix to acrylamide, (of course I don't remember
>who or where) which they claimed improved resolution, does anyone
>out there know what I am talking about?  Thanks in advance, Morrie 

You may be thinking of the Long Ranger gel reagent. It is sold by
J.T. Baker (the big chemical company). I don't know if they actually make
it or buy from someone else. You can get it from VWR and probably from other
companies also. 

I have never tried so don't take this as a recommendation...


----------------------------------------------------------------------
 Michael Benedik				INTERNET: Benedik@uh.edu
 Dept. of Biochemical & Biophysical Sciences	
 University of Houston				BITNET: Benedik@uhou
-----------------------------------------------------------------------
From: jaishre@matt.ksu.ksu.edu (Jaishree Muppala Chittoor)
Newsgroups: bionet.molbio.methds-reagnts
Subject: Northern Blots
Date: 8 Nov 1992 22:01:18 -0600
Organization: Kansas State University
NNTP-Posting-Host: matt.ksu.ksu.edu

Help!  I am seeing a very intense background in all my blots, recently.  The 
hybridization protocol that I used was working properly (with no background) 
in all of my earlier blots. And now, all of a sudden, I am having this problem. 

I can't think of any parameter that I have knowing changed to account for the 
problem.  I have been using the same blot (magna, high density), probe, prehyb
solution (0.1% SDS, 0.05M phosphate buffer pH 7.0, 1M NaCl and 300u/ml salmon 
sperm DNA) with a prehyb time > 5hrs at 65 C in all my work.  I generally do 
hyb: overnight at 65 and washes with 2xSSC at 65. 

I changed blot (to gene screen plus), changed probe and even used a different 
pre hyb solution (5x SSC, 5x denhart, 0.5%SDS, 20mM phosphate buffer, 300u/ml 
salmon sperm DNA, 5mM EDTA), but nothing seems to eliminate the very intense 
background.  I have even tried washing with water.

I would greatly appreciate some help that will help me to solve this problem.

Bye
Jaishree [jaishre@matt.ksu.ksu.edu or jaishre@ksuvm.ksu.edu]

A
A
A
A
From: MCAO@VTHVAX.TAMU.EDU
Newsgroups: bionet.molbio.methds-reagnts
Subject: RE: Which salt of X-P to use for detection of alkaline phosphatase?
Date: 12 Nov 92 15:13:05 GMT
Distribution: bionet

We use the toluidine salt for our alkaline phosphatase reaction. Sigma has both
salts and can anwser this question.
From: djt2@po.CWRU.Edu (Dennis J. Templeton)
Newsgroups: bionet.molbio.methds-reagnts
Subject: Re: Western Blot staining
Date: 12 Nov 92 23:06:34 GMT
Reply-To: djt2@po.CWRU.Edu (Dennis J. Templeton)
Organization: Case Western Reserve University, Cleveland, OH (USA)
NNTP-Posting-Host: thor.ins.cwru.edu


In a previous article, sandy@pt.Cyanamid.COM (Sandy Silverman) says:

>We once read about a method to detect protein on a Western blot prior
>to antibody detection.  It involved a red dye and acetic acid.  It was 
>reversible.  Does anyone have the reference?   Thanks-----Sandy
>
We use a recipe that is simpler than the Sambrook one:

0.5% Ponseau Red
1% Acetic acid
in water

We stain Immobilon filters for ~1 min and then rinse under the DI water tap 
for about 1 minute.  The limit of sensitivity is about 200-400 ng, or 
maybe worse.

On immobilon, you can detect proteins even more sensitively by simple
observation; there is a shiny-ness that is evident at the right angle. 
Personally, I mark my lanes and marker proteins this way, but others in my
lab can't see it and prefer the ponseau stain.

good luck,

dennis
Newsgroups: bionet.molbio.methds-reagnts
From: pnh@fcsparc6.ncifcrf.gov (Paul N Hengen)
Newsgroups: bionet.molbio.methds-reagnts
Subject: Re: Northern Blots
Summary: Just a thought...
Date: 9 Nov 92 22:39:31 GMT
Organization: Frederick Cancer Research and Development Center

Jaishree Muppala Chittoor writes:

>Help!  I am seeing a very intense background in all my blots, recently.  The 
>hybridization protocol that I used was working properly (with no background) 
>in all of my earlier blots. And now, all of a sudden, I am having this problem. 

This is just a crazy thought, but the first thing I would do is:

1. Make sure someone hasn't recently replaced the safety lamp in
the dark room.

2. Start with all fresh developing solutions for the X-ray film.
Did you say you were using radioactivity? Run an unexposed piece
of film through to make certain.

Paul N. Hengen
National Cancer Institute
Frederick Cancer Research and Development Center
Frederick, Maryland 21702-1201 USA
Newsgroups: bionet.molbio.methds-reagnts
From: jgill@kean.ucs.mun.ca
Subject: hybridization problems (update)
Organization: Memorial University. St.John's Nfld, Canada
Date: Mon, 9 Nov 1992 13:42:07 GMT

Hi All:

About a month ago I posted a letter entitled Hybridization Problems.  
In this letter I stated that I was having trouble with my northern and 
slot blots, wherby I was getting a negative signal on my autorad.  
First of all I would like to thank all of you for your suggestions and 
say that my problem has recently been resolved.  I changed my Nylon 
N(+) membrane to the Zetaprobe GT by Biorad.  Now my bands are showing 
up just lovely for both Northern and Slot Blots.  Also I found that I 
get very low background with oligos as well as my cDNA probe.

Thanx Juan
Newsgroups: bionet.molbio.methds-reagnts
From: khofmann@biomed.biolan.uni-koeln.de
Subject: Re: cDNA cloning artifact help or comment
Reply-To: khofmann@biomed.biolan.uni-koeln.de
Organization: Biochemical Institute, Cologne University
Distribution: bionet
Date: Mon, 09 Nov 92 13:45:17 GMT

In article <1992Nov6.093054.746@gserv1.dl.ac.uk>, IBELGAUFTS@vms.biochem.mpg.de writes:
>dear colleagues,
>I have isolated a cDNA clone from a brain cDNA library.
>This clone contains a 3 kb ORF which perfectly matches a known
>gene which we are investigating. At one end of the ORF there is a
>short 150 bp fragment which does not have an ORF and is not related
>to the 3 kb sequence. A search of databases shows that these 15o bp
>are not homologous to any known sequence.
>Does anyone out there have an explanation for this or could point me
>to literature describing similar observations?

Things like that happen to us rather frequently. My assumption always has
been that these are artifacts of the cDNA library construction. Perhaps
more than one insert has been ligated into the lambda arms.
We observed this kind of clone-fusion only in commercial available cDNA
libraries, home-made libraries were found to be ok in all cases up to now.

A certain library we had LOTS of troubles with was a rat brain cDNA library
from stratagene, what library are you using?

Best regards,
                 Kay
From: cytogen@chmeds.ac.nz (Martin Kennedy)
Newsgroups: bionet.molbio.methds-reagnts
Subject: Re: Northern Blots
Date: 12 Nov 92 05:14:56 GMT

In article <BxH09v.C95@ncifcrf.gov>, pnh@fcsparc6.ncifcrf.gov (Paul N Hengen) writes:
> Jaishree Muppala Chittoor writes:
> 
>>Help!  I am seeing a very intense background in all my blots, recently.  The 
>>hybridization protocol that I used was working properly (with no background) 
>>in all of my earlier blots. And now, all of a sudden, I am having this problem. 
> 
> This is just a crazy thought, but the first thing I would do is:
> 
> 1. Make sure someone hasn't recently replaced the safety lamp in
> the dark room.
> 
> 2. Start with all fresh developing solutions for the X-ray film.
> Did you say you were using radioactivity? Run an unexposed piece
> of film through to make certain.
 
> Paul N. Hengen

It should be easy to distinguish between film problems vs blot bg:  only the
area covered by the blot will be black if it is background.
From: djt2@po.CWRU.Edu (Dennis J. Templeton)
Newsgroups: bionet.molbio.methds-reagnts
Subject: Retrovirus mutagenesis
Date: 6 Nov 92 03:57:31 GMT
Reply-To: djt2@po.CWRU.Edu (Dennis J. Templeton)
Organization: Case Western Reserve University, Cleveland, OH (USA)
NNTP-Posting-Host: slc5.ins.cwru.edu


We're interested in selecting for a mutant of a gene inserted into a
retroviral vector, so that the mutant conferrs a selectable function (cell
transformation)  The plan is to mutagenize the producer cell line, then
take the viral supernatant and assay for transforming activity.  We're
planning NitrosoUrea mutagenesis of the cell line.

It has occurred to me recently that it might be better for us to produce
mutagenized RNA transcripts (i.e. the viral genome) rather than
mutangenizing  the producer cell DNA with alkylating agents.  What I imagine
is some sort of base analog that might be mis-incorporated into the
transcribed RNA.  It seems that at an equivalent level of "mutagenesis" of
the retroviral genomes, the toxic effects on the cells should be much
lower.  

There is a reasonable literature on mis-incorporation mutagens in DNA
polymerases, but I am not aware of any on RNA polymerases, let alone any
studies done in vivo.  I'm hoping that some RNA worker out there might have
advice or some references.  If I get more than a couple of responses, I'll
summarize here...


thanks, 

dennis
Newsgroups: bionet.molbio.methds-reagnts
From: broe@aardvark.ucs.uoknor.edu (Bruce Roe)
Subject: Re: sequencing from 300-500 bp
Originator: usenet@midway.ecn.uoknor.edu
Date: Thu, 12 Nov 1992 12:31:00 GMT
News-Software: VAX/VMS VNEWS 1.41    
Organization: University of Oklahoma - University Computing Services

In article <1992Nov12.061933.23712@menudo.uh.edu>, bchs1b@Elroy.UH.EDU writes...
>In article <Af0KlOu00iV005CmZv@andrew.cmu.edu>, "Morris F. Manolson" <mm6y+@andrew.cmu.edu> writes:
>>Dear Netters,  I am sure someone must have asked this before but......
>>
>>I am trying to sequence off of one reaction 300 to 500 bps using the 
>>standard IBI or BRL 44 cm glates.  Without having to resort to ultra-long
>>plates (of which I don't have), does anyone have any suggesting on how to
>>improve the resolution of the bands in the 300 to 500 bp region.  My reactions
>>are fine and I can see bands up to 600 bp from the primer, I just can't
>>resolve them well enough to read.  I am using 6% acrylamide wedge gels and run
>>them for 24 hours at 900 Volts, this gives me nice sequence on average
>>up to 350 bp,  on a great gel I can get 400.  I remember some company trying
>>to sell an alternative matrix to acrylamide, (of course I don't remember
>>who or where) which they claimed improved resolution, does anyone
>>out there know what I am talking about?  Thanks in advance, Morrie 
> 
>You may be thinking of the Long Ranger gel reagent. It is sold by
>J.T. Baker (the big chemical company). I don't know if they actually make
>it or buy from someone else. You can get it from VWR and probably from other
>companies also. 
> 
>I have never tried so don't take this as a recommendation...
> 
> 
>----------------------------------------------------------------------
> Michael Benedik				INTERNET: Benedik@uh.edu
> Dept. of Biochemical & Biophysical Sciences	
> University of Houston				BITNET: Benedik@uhou
>-----------------------------------------------------------------------

Lower the acrylamide to 4%, increase electrophoresis time and eliminate
the wedge.  See for example:

   Johnston-Dow, L., Mardis, E., Heiner, C., and Roe, B.A. (1987) 
   BioTechniques 5, 754-765.
   and references to Ansorge's papers.

But, even though you can see these bands and somewhat resolve them,  you
may have difficulty accurately reading them, especially when there are
runs of 3-4 identical bases.
Newsgroups: bionet.molbio.methds-reagnts
From: news@massey.ac.nz (USENET News System)
Subject: Dewaxing
Organization: AgResearch
Date: Tue, 10 Nov 92 04:33:56 GMT

 Hi.
I want to dewax some paraffin embedded tissue for in situs. I've previously
only used  Histoclear which is not readily available in this far flung corner
of the world. Inhibisol (BDH), has been sugested as an alternative. Has anyone
had experience with this metal cleaner? (At least it does not smell of oranges)

Thanks in anticipation
Murray Grant