From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Pyrus communis polygalacturonase inhibitor mRNA, complete cds.
Date: 27 Jan 93 05:51:06 GMT
Distribution: bionet
Checksum: 46731 4

LOCUS       PYPPGI       1057 bp ss-mRNA            PLN       26-JAN-1993
DEFINITION  Pyrus communis polygalacturonase inhibitor mRNA, complete cds.
ACCESSION   L09264
KEYWORDS    polygalacturonase inhibitor.
SOURCE      Pyrus communis (cultivar Bartlett) immature fruit fruit cDNA to
            mRNA.
  ORGANISM  Pyrus communis
            Eukaryota; Plantae; Embryobionta; Magnoliophyta; Magnoliopsida;
            Rosidae; Rosales; Rosaceae.
REFERENCE   1  (bases 1 to 1057)
  AUTHORS   Abu-Goukh,A.A., Greve,L.C. and Labavitch,J.M.
  TITLE     Purification and partial characterization of 'Bartlett' pear
            polygalacturonase inhibitors
  JOURNAL   Physiol. Plant Pathol. 23, 111-122 (1983)
  STANDARD  full automatic
REFERENCE   2  (bases 1 to 1057)
  AUTHORS   Stotz,H.U., Powell,A.L., Damon,S.E., Greve,L.C., Bennett,A.B. and
            Labavitch,J.M.
  TITLE     Molecular characterization of a polygalacturonase inhibitor from
            Pyrus communis L. cv. 'Bartlett'
  JOURNAL   Plant Physiol. (1993) In press
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     sig_peptide     34..105
                     /gene="PGIP"
                     /codon_start=1
     mat_peptide     106..1023
                     /function="inhibitor of fungal polygalacturonases"
                     /product="polygalacturonase inhibitor"
                     /codon_start=1
                     /citation=[1]
     CDS             34..1026
                     /function="precursor of an inhibitor of fungal
                     polygalacturonase"
                     /gene="PGIP"
                     /label=CDS
                     /product="polygalacturonase inhibitor"
                     /codon_start=1
                     /translation="MELKFSTFLSLTLLFSSVLNPALSDLCNPDDKKVLLQIKKAFGD
                     PYVLASWKSDTDCCDWYCVTCDSTTNRINSLTIFAGQVSGQIPALVGDLPYLETLEFH
                     KQPNLTGPIQPAIAKLKGLKSLRLSWTNLSGSVPDFLSQLKNLTFLDLSFNNLTGAIP
                     SSLSELPNLGALRLDRNKLTGHIPISFGQFIGNVPDLYLSHNQLSGNIPTSFAQMDFT
                     SIDLSRNKLEGDASVIFGLNKTTQIVDLSRNLLEFNLSKVEFPTSLTSLDINHNKIYG
                     SIPVEFTQLNFQFLNVSYNRLCGQIPVGGKLQSFDEYSYFHNRCLCGAPLPSCK"
BASE COUNT      268 a    341 c    197 g    251 t
ORIGIN      
        1 acatctctca ggctctcaac caaaaccaaa acaatggaac tcaagttctc caccttcctc
       61 tccctaaccc tactcttctc ctccgtccta aaccccgctc tctccgatct ctgcaacccc
      121 gacgacaaaa aagtcctcct acaaatcaag aaagccttcg gcgaccccta cgtcttggcc
      181 tcatggaaat cagacactga ctgctgcgat tggtactgcg tcacctgtga ctccaccaca
      241 aaccgcatta actccctcac catctttgcc ggccaggtgt caggccaaat ccccgcccta
      301 gtaggagact tgccatacct tgaaaccctt gaattccata agcaacccaa tctcactggc
      361 ccaatccaac ccgccattgc caagctcaaa ggactcaagt ctctcaggct cagctggacc
      421 aacctctcag gctctgtccc tgacttcctc agccaactca agaacctcac attcctcgac
      481 ctctccttca acaacctcac cggtgccatc cccagctcgc tttctgagct cccaaacctc
      541 ggcgctcttc gtctagaccg caataagctc acaggtcata ttccgatatc gtttgggcag
      601 ttcattggca acgttccaga cctgtatctc tcccacaacc agctttctgg taacattcca
      661 acctcattcg ctcagatgga cttcaccagc atagacttat cacggaacaa gctcgaaggt
      721 gacgcatccg tgatatttgg gctgaacaag acaacccaga ttgtggacct gtccaggaac
      781 ttgctggaat ttaatctgtc aaaggtggag tttccgacaa gcttgacctc gctggatatc
      841 aaccacaata agatctacgg gagtatccca gtggagttta cgcaactgaa tttccagttc
      901 ctgaacgtga gctacaacag gctgtgtggt cagattcctg tgggtggaaa gttgcagagc
      961 ttcgacgagt attcttattt ccataaccga tgcttgtgcg gtgctccact cccaagctgc
     1021 aagtaaaggc cacaactgca gatttggcca gcaattt
//