From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Saccharomyces cerevisiae vacuolar H+-ATPase subunit (VPH1) gene,
Date: 24 Jul 92 10:11:03 GMT
Distribution: bionet
Checksum: 28928     7

LOCUS       YSCVPH1P     3046 bp ds-DNA             PLN       24-JUL-1992
DEFINITION  Saccharomyces cerevisiae vacuolar H+-ATPase subunit (VPH1) gene,
            complete cds.
ACCESSION   M89778
KEYWORDS    H+-ATPase subunit; vacuolar H+-ATPase subunit.
SOURCE      Saccharomyces cerevisiae (strain X2180-1b) (library: YCp50) DNA.
  ORGANISM  Saccharomyces cerevisiae
            Eukaryota; Fungi; Eumycota; Hemiascomycetes; Endomycetales;
            Saccharomycetaceae.
REFERENCE   1  (bases 1 to 3046)
  AUTHORS   Manolson,M.F., Proteau,D., Preston,R.A., Stenbit,A., Roberts,B.T.,
            Hoy,M.A., Preuss,D., Mulholland,J., Botstein,D. and Jones,E.W.
  TITLE     The VPH1 gene encodes a 95-kDa integral membrane polypeptide
            required for in Vivo assembly and activty of the yeast vacuolar
            H+-ATPase
  JOURNAL   J. Biol. Chem. 267, 14294-14303 (1992)
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     CDS             487..3009
                     /function="essential for in vivo assembly of yeast
                     vacuolar H+-ATPase"
                     /EC_number="3.6.1.3"
                     /standard_name="Vph1p"
                     /note="95 kDa subunit"
                     /product="vacuolar H+-ATPase subunit"
                     /gene="VPH1"
                     /evidence=EXPERIMENTAL
                     /label=V-ATPase
                     /codon_start=1
                     /translation="MAEKEEAIFRSAEMALVQFYIPQEISRDSAYTLGQLGLVQFRDL
                     NSKVRAFQRTFVNEIRRLDNVERQYRYFYSLLKKHDIKLYEGDTDKYLDGSGELYVPP
                     SGSVIDDYVRNASYLEERLIQMEDATDQIEVQKNDLEQYRFILQSGDEFFLKGDNTDS
                     TSYMDEDMIDANGENIAAAIGASVNYVTGVIARDKVATLEQILWRVLRGNLFFKTVEI
                     EQPVYDVKTREYKHKNAFIVFSHGDLIIKRIRKIAESLDANLYDVDSSNEGRSQQLAK
                     VNKNLSDLYTVLKTTSTTLESELYAIAKELDSWFQDVTREKAIFEILNKSNYDTNRKI
                     LIAEGWIPRDELATLQARLGEMIARLGIDVPSIIQVLDTNHTPPTFHRTNKFTAGFQS
                     ICDCYGIAQYREINAGLPTIVTFPFMFAIMFGDMGHGFLMTLAALSLVLNEKKINKMK
                     RGEIFDMAFTGRYIILLMGVFSMYTGFLYNDIFSKTMTIFKSGWKWPDHWKKGESITA
                     TSVGTYPIGLDWAWHGTENALLFSNSYKMKLSILMGFIHMTYSYFFSLANHLYFNSMI
                     DIIGNFIPGLLFMQGIFGYLSVCIVYKWAVDWVKDGKPAPGLLNMLINMFLSPGTIDD
                     ELYPHQAKVQVFLLLMALVCIPWLLLVKPLHFKFTHKKKSHEPLPSTEADASSEDLEA
                     QQLISAMDADDAEEEEVGSGSHGEDFGDIMIHQVIHTIEFCLNCVSHTASYLRLWALS
                     LAHAQLSSVLWTMTIQIAFGFRGFVGVFMTVALFAMWFALTCAVLVLMEGTSAMLHSL
                     RLHWVESMSKFFVGEGLPYEPFAFEYKDMEVAVASASSSASS"
                     /citation=[1]
BASE COUNT      930 a    522 c    610 g    984 t
ORIGIN      Chromosome XV
        1 agaagtgaag agacaaattt atatagttat agaataaata tcagatagat aagaaaaaaa
       61 aattagttaa acattaatat atatatatgt gtagtgactg acatacgtat gactgctagt
      121 aatccagttg ccgagctatt gttgcagatt gaaatgtcat cacgtggaca ttatatcatc
      181 attaacgact aatgcgtcaa aaaaaaaatg ggctaaaaaa aaaaaaagca aaaaaaagaa
      241 actcagataa gtcgatatgg aaaacctttt ttcttgagtg caaccctttt agaggttaca
      301 aaacaacaaa agtccaaata agtccatagc ttttgcctct caatatatct atacttgctt
      361 agagggctac ctgtgtgtat ttgctagctt ttacctttct ctatacaaca agtgagggtt
      421 tgaactttta aatccctttt aatttgaatc aaaaaaaaaa catttaaagg ttacacaagg
      481 aaaataatgg cagagaagga ggaagcgatt tttcgctctg ctgaaatggc tttagtccaa
      541 ttctatattc ctcaagaaat ttcaagagac tctgcttaca ctttaggtca attgggtctt
      601 gttcaattcc gtgacttgaa ctctaaggtg cgtgcgtttc aaagaacttt cgtgaacgaa
      661 attagaagac tggataatgt agaaagacag tatcgttact tttattctct tttgaagaaa
      721 cacgatatta agctctacga aggagacacg gacaaatatt tggacggctc aggtgaattg
      781 tacgttccac caagcggttc agtgatagat gattatgtcc ggaacgcttc atatttggaa
      841 gaaagattga ttcaaatgga ggatgcaacc gatcaaattg aagtccagaa aaatgacttg
      901 gaacagtatc gctttatttt gcagtcaggt gatgaatttt tcttgaaggg tgataatacc
      961 gacagcactt cctatatgga tgaagacatg atcgacgcta atggggaaaa cattgctgct
     1021 gctatcggtg cttctgtaaa ctatgtcact ggtgtcattg ctagagacaa agttgccacc
     1081 ttagaacaaa ttctttggag agtattaaga ggtaaccttt tcttcaaaac tgttgaaatt
     1141 gaacaacctg tttatgatgt caaaaccagg gagtataaac ataaaaatgc ttttatcgta
     1201 ttttctcacg gtgatctgat tattaaaaga atcagaaaga ttgcggaatc attggatgcc
     1261 aatctttacg atgttgactc ttccaacgag ggtagatcac aacaattggc caaggtcaac
     1321 aagaatttga gtgatttgta cacagttttg aaaaccactt ctaccacttt agaaagtgaa
     1381 ttatatgcca ttgccaaaga attggactct tggttccaag atgttacccg tgaaaaggcg
     1441 atttttgaaa ttttgaacaa gtctaactat gataccaata gaaagatttt gattgctgaa
     1501 ggttggatac caagagacga attggctact ttgcaagctc gtcttggtga aatgatcgca
     1561 agattgggta ttgatgtccc atccattatc caagtcctgg atacaaacca cactccacct
     1621 accttccaca gaactaacaa gtttactgct ggtttccaaa gtatctgtga ctgttacggt
     1681 attgctcagt acagagaaat caatgctggt ttacccacaa ttgtcacttt ccctttcatg
     1741 tttgccatca tgtttggtga tatgggtcac gggttcttaa tgaccttagc cgcattgtct
     1801 cttgtattga atgaaaagaa aatcaacaaa atgaaaagag gcgaaatttt cgatatggcc
     1861 ttcactggta gatacattat tttgttgatg ggtgtctttt ccatgtacac aggtttcctt
     1921 tacaacgata tcttctctaa aactatgact attttcaagt ctggttggaa atggcctgat
     1981 cattggaaaa aaggtgagag tattactgct acatcggtgg gtacataccc tatcggttta
     2041 gattgggctt ggcatggaac tgaaaatgct ttgttatttt ctaattctta caaaatgaaa
     2101 ctatcaattt taatggggtt catccacatg acctattctt atttcttttc gttggctaac
     2161 cacctatact ttaactctat gattgatatc atcggtaact ttattcctgg tttgctattt
     2221 atgcaaggta tctttggtta tctttccgtt tgtattgttt acaaatgggc tgttgattgg
     2281 gttaaggacg gaaagcctgc tccaggtttg ttaaatatgt tgatcaacat gtttttatca
     2341 ccaggaacta ttgacgatga attataccct catcaagcaa aggtccaagt gtttttgttg
     2401 ttgatggcct tggtttgtat tccttggttg ctattggtga agccattaca tttcaaattc
     2461 actcataaaa agaaatctca cgaaccactg ccatcgactg aagcagatgc tagttctgaa
     2521 gatttggaag cacaacaatt aatttccgcg atggacgccg atgacgctga agaagaagaa
     2581 gttggttctg gatctcatgg tgaagacttt ggtgatatta tgattcatca agttattcat
     2641 acaattgaat tctgtttgaa ttgtgtttcg cacactgcat cctatttacg tttatgggcc
     2701 ttatcattgg cacatgctca attgtctagt gttttatgga caatgacaat tcaaattgcc
     2761 tttggattta gaggatttgt gggtgtgttt atgacggttg cactttttgc catgtggttc
     2821 gcactaacat gtgcagttct tgttttgatg gaaggtacat ctgccatgct tcattcctta
     2881 cgtttgcact gggttgaatc tatgtccaag tttttcgtgg gtgaaggttt accatacgaa
     2941 ccattcgcat ttgagtataa agacatggaa gtcgctgttg ctagtgcaag ctcttccgct
     3001 tcaagctaaa ttttgaggac ttttaaaaaa agcgaaacat ttaagt
//