From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Leishmania enriettii multidrug resistant protein (mdr1) gene,
Date: 3 Jan 93 22:07:10 GMT
Distribution: bionet
Checksum: 57190 9

LOCUS       LEIMDRI      4378 bp ds-DNA             INV       21-DEC-1992
DEFINITION  Leishmania enriettii multidrug resistant protein (mdr1) gene,
            complete cds.
ACCESSION   L08091
KEYWORDS    multidrug resistance protein.
SOURCE      Leishmania enriettii (library: lambda fix) promastigote DNA.
  ORGANISM  Leishmania enriettii
            Eukaryota; Animalia; Protozoa; Sarcomastigophora; Mastigophora;
            Kinetoplastida; Trypanosomatina; Trypanosomatidae.
REFERENCE   1  (bases 1 to 4378)
  AUTHORS   Chow,L.M., Wong,A.K., Ullman,B. and Wirth,D.F.
  TITLE     Amplification of an Multi-Drug-Resistance (mdr) like gene in
            vinblastine-resistant Leishmania enriettii
  JOURNAL   Unpublished (1993)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     mat_peptide     285..4124
                     /product="multidrug resistance protein"
                     /gene="mdr1"
                     /codon_start=1
                     /citation=[1]
                     /note="putative"
     CDS             285..4127
                     /gene="mdr1"
                     /citation=[1]
                     /note="putative"
                     /product="multidrug resistance protein"
                     /codon_start=1
                     /translation="MSRAHAAYANEGWSAPEGGIAGKDGSTRDCSGYGSQGPLFSAEE
                     EVKGTVVRETVGPIEIFRYADATDRVLMIAGTAFAVACGAGMPVFSFIFGRIAMDLMS
                     GVGSAEEKAAKTSLIMVYVGIAMLIACAGHVMCWTVAACRQVARIRLLFFRAVLRQDI
                     GWHDEHSPGALTARMTGDTRVIQNGINDKLSQGIMNGSMGVIGYIAGFVFSWELTLMM
                     IGMMPFIIVMAAIIGSIVSKITESSRKYFAKAGSLATEVMENIRTVQAFGREDYELER
                     FTKAVLYAQGRGIRKELASNLSAAVIMALMYVSYTVAFFFGSYLVEWGRRDMADIIST
                     FLAVLMGSFGLGFVAPSRTAFTESRAAAYEIFKAIDRVPPVDIDAGGVPVPGFKESIE
                     FRNVRFAYPTRPGMILFRDLSLKIKCGQKVAFSGASGCGKSSVIGLIQRFYDPIGGAV
                     LVDGVRMRELCLREWRDQIGIVSQEPNLFAGTMMENVRMGKPNATDEEVVEACRQANI
                     HDTIMALPDRYDTPVGPVGSLLSGGQKQRIAIARALVKRPPILLLDEATSALDRKSEM
                     EVQAALDQLIQRGGTTVVVIAHRLATIRDMDRIYYVKHDGAEGSRITESGTFDELLEL
                     DGEFAAVAKMQGVLAGDAKSGASVRDAKKASGHLGVILDEADLAQLDEDVPRTARQNV
                     PIDELAKWEVKHAKVGFLRLMRMNKDKAWAVALGILSSVVIGSARPASSIVMGHMLRV
                     LGEYSATKDVEALRSGTNLYAPLFIVFAVANFSGWILHGFYGYAGEHLTTKIRVLLFR
                     QIMRQDINFFDIPGRDAGTLAGMLSGDCEAVHQLWGPSIGLKVQTMCIIASGLVVGFI
                     YQWKLALVALACMPLMIGCSLTRRLMINGYTKSREGDTDDTIVTEALSNVRTVTSLNM
                     KEDCVEAFQAALREEAPRSVRKGIIAGGIYGITQFIFYGVYALCFWYGSKLIDKGEAE
                     FKDVMIASMSILFGAQNAGEAGAFATKLADAEASAKRVFSVIDRVPDVDIEQAGNKDL
                     GEGCDIEYRNVQFIYSARPKQVVLASVNMRFGDATSNGLIGQTGCGKSTVIQMLARFY
                     ERRSGLISVNGRDLSSLDIAEWRRNISIVLQEPNLFSGTVRENIRYAREGATDEEVEE
                     AARLAHIHHEIIKWTDGYDTEVGYKGRALSGGQKQRIAIARGLLRRPRLLLLDEATSA
                     LDSVTEAKVQEGIEAFQAKYKVTTVSIAHRLTTIRHCDQIILLDSGCIIEQGSHEELM
                     ALGGEYKTRYDLYMSALS"
BASE COUNT      843 a   1132 c   1475 g    928 t
ORIGIN      
        1 cttcttcttc gttcttctgc tggctggggg gggacagaag aacgaacagt accgtcttct
       61 ctctaaagac tccactatcg acacgtgcac gccggtgacg tcatgcctaa gagtccgaca
      121 agcgatgcaa gagccaaggg cagctctgtt ttcgagagcg aggacagtca ggaggcgttt
      181 tgtcctcacc agcccatcga tgagggtcta ttggaacgca gggcttcgcc gatcgcagcg
      241 acaacagctg cagtcaggca ccgttgtcta agggggcgat caatatgtcg cgcgcacatg
      301 ctgcgtatgc gaatgagggc tggtcggctc ccgagggcgg aattgctggt aaggatggta
      361 gcacgcgcga ctgcagcggc tacggcagcc aaggtcctct attcagcgcc gaggaggagg
      421 tcaaggggac ggtggtgcgc gaaaccgtag gtccgataga aatcttccgc tacgccgacg
      481 cgactgaccg tgtgttgatg atcgctggca ccgcctttgc ggtcgcctgc ggtgccggca
      541 tgcccgtgtt ctcgttcatc tttggtcgca tcgcaatgga tctcatgtca ggagtgggaa
      601 gtgcggagga aaaagcggcg aagacgtcgc tgatcatggt gtacgttggc attgctatgc
      661 tcatcgcctg tgctggtcac gtgatgtgtt ggaccgtggc cgcatgccgt caggtagcgc
      721 gcatccgtct gctcttcttt cgtgctgtgc tgcgccagga cattgggtgg cacgacgagc
      781 acagtcctgg agcgctgaca gctcgcatga cgggcgacac gcgtgtgata cagaacggta
      841 tcaacgacaa gctgtcgcag ggtatcatga acggatccat gggtgtcatt ggctatattg
      901 ccggctttgt gttttcgtgg gagctgacgc ttatgatgat cggcatgatg cccttcatta
      961 ttgttatggc tgctatcatt ggcagcatcg tgtccaagat cacagagtcg tcgcgcaagt
     1021 acttcgcaaa ggctggatct ctggcaacag aggtgatgga gaacatccgc acggtgcagg
     1081 cgtttggccg ggaggactat gagttggagc gcttcaccaa agcggttctg tatgcccagg
     1141 gccgtggcat ccgcaaagag ttggcaagca acctttcagc agcagtgatc atggccctca
     1201 tgtatgtcag ctacaccgtt gcctttttct tcggctccta cctcgtcgaa tggggtcgcc
     1261 gcgacatggc agacatcatt tcaacctttc tggctgtgtt gatgggtagc ttcggtctcg
     1321 ggtttgtggc gccgagccgg actgcattca ccgagtcccg cgctgctgcc tacgagatct
     1381 tcaaggcgat cgaccgtgtg ccgccggtag acatcgacgc tggcggcgtc cctgtgccgg
     1441 gcttcaagga gagcatcgag ttccgcaatg tgcggttcgc gtacccgact cgcccgggca
     1501 tgatcctatt tcgagatcta agcctgaaga tcaagtgtgg gcagaaggtg gcgttctctg
     1561 gcgcttccgg gtgcggcaag tcgtcggtga tcgggctgat tcagcgcttc tacgacccga
     1621 ttggcggcgc cgtgctcgtc gatggcgtac gcatgcgtga gctgtgcctg cgcgaatggc
     1681 gtgaccagat tgggatcgtg tcgcaggagc ccaacctgtt tgcggggaca atgatggaga
     1741 acgtacgcat ggggaagccg aacgcgacgg acgaggaagt ggtcgaggca tgcaggcagg
     1801 cgaacatcca tgacacgatc atggctctgc cggaccggta cgacacgcct gtggggcctg
     1861 tgggctcgct gctctcgggt gggcagaagc agcgcatcgc catcgcgcgc gcgctcgtga
     1921 agcggccgcc gatcctactg ctggacgagg caacgagcgc cctcgaccgc aagtccgaaa
     1981 tggaggtgca ggcagcactg gatcagctga tacagagggg cgggacgact gtggtcgtga
     2041 ttgcgcaccg gctcgcgacg atccgagaca tggatcgcat ctactacgtg aagcacgacg
     2101 gcgcggaggg gagccggatc acggagagcg ggacgtttga cgagttgctg gagcttgatg
     2161 gagagttcgc ggcggtggcg aagatgcaag gcgtacttgc tggtgacgca aagagtggcg
     2221 cgagtgtgcg cgacgcgaag aaggcgagcg gccacctggg tgtgatcctg gacgaggcgg
     2281 atcttgcgca actggacgag gatgtgccac gaacggcgcg tcagaatgtg ccgatcgacg
     2341 agctcgcaaa gtgggaagta aagcacgcga aggtcggctt cttgcggtta atgagaatga
     2401 acaaagacaa ggcgtgggca gttgctttgg gtatcctcag ctccgtggtg attggctctg
     2461 cccggcctgc gagctccatc gtgatgggtc acatgctgcg tgtgcttggc gagtacagtg
     2521 ctaccaagga tgttgaagca ctgcgcagcg gcaccaatct gtacgcgcca ctgtttatcg
     2581 tctttgcggt tgcgaatttc tcgggttgga ttctgcacgg gttttacggc tacgccggtg
     2641 agcatctgac aacgaagatc cgcgtgctgc tgtttcgcca gatcatgcgc caggacatta
     2701 acttcttcga catacccgga cgcgatgccg gcaccctggc tgggatgttg tccggcgact
     2761 gcgaggccgt ccaccagttg tggggcccat cgatcggcct gaaggtgcaa acgatgtgta
     2821 tcatcgcttc cggactggtg gtgggcttca tctaccagtg gaagcttgcg ttagtggcgc
     2881 tggcctgcat gccactcatg atcggctgca gtcttacgag gcgcctgatg atcaatgggt
     2941 atacgaagag ccgcgaaggt gacacggatg acacgatcgt gacggaggcg ctgtcgaatg
     3001 tgcgcacggt gacgtcgctc aacatgaagg aggattgcgt agaggccttc caagcggcat
     3061 tgcgagagga ggcgccgcgc agtgtgagga agggcattat cgctggcggc atctacggca
     3121 tcacgcagtt tattttttac ggcgtttacg cactgtgctt ctggtatggc agcaagctca
     3181 tcgacaaggg tgaggctgaa ttcaaggatg tcatgattgc aagcatgtca atactattcg
     3241 gtgcgcagaa cgctggtgag gccggcgcgt tcgcgacgaa gcttgcggat gcggaggctt
     3301 ctgcgaagcg tgtattcagc gtgattgacc gggtgccgga cgtggacata gagcaggccg
     3361 ggaacaaaga cttgggtgag ggctgcgaca tcgagtaccg aaatgtgcag ttcatctact
     3421 cggcgcggcc gaagcaggtc gtgcttgcgt ccgtcaacat gcgcttcggc gacgcgacct
     3481 cgaacgggtt gatagggcag acggggtgcg ggaagtcgac ggtcatccag atgcttgcgc
     3541 gtttctacga gaggcgctct gggctgatct ccgtgaatgg gagggacctt tcctcgctgg
     3601 acatcgcgga gtggcggcgt aacatcagca ttgtactcca ggagccgaat ctgtttagcg
     3661 ggaccgttcg ggaaaacatc cgctatgcgc gcgagggcgc gacagacgag gaggtggagg
     3721 aggctgcacg gctggcgcac attcaccacg agatcataaa gtggacggat gggtacgata
     3781 cagaggtggg gtacaagggt cgcgcgctat ctggtggcca gaaacagcgc attgcgattg
     3841 cgcgcgggct gctgcggcgg ccaaggctgc tgctgctgga cgaggcaacg agcgcgctgg
     3901 acagcgtgac ggaggcgaag gtgcaggagg gcatcgaagc gttccaggca aagtacaagg
     3961 tcacgacggt gagcattgcg catcggttga cgacaatccg acactgtgac cagatcatcc
     4021 tgctggactc tgggtgcatc attgagcagg gcagccacga agagctgatg gcgcttggcg
     4081 gcgagtacaa gacgcgttac gacctgtaca tgagtgcact ctcatgatga tgggtggcga
     4141 gctgcgctgt tgccgtttgc ggcgctactc gcggcggcgc gtctttcttt gcacgcggca
     4201 gtgctctgtc tgctgacgac ttcttcgctg tgactgtgtt tcgctccacc gggcctgatg
     4261 gctgcctttg tacggcgcgt ctggtcgctg gccttcgggg tgacgcagct cctccatggt
     4321 gcctttctct ttccatccgt cgcttcgggc tttgtacgag cagcgtggtg ctgtgttt
//