From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human nonspecific crossreacting antigen mRNA, complete cds.
Date: 26 Aug 92 10:00:36 GMT
Distribution: bionet
Checksum: 64975     6

LOCUS       HUMANTNCA    2527 bp ss-mRNA            PRI       26-AUG-1992
DEFINITION  Human nonspecific crossreacting antigen mRNA, complete cds.
ACCESSION   M18216 J03550
KEYWORDS    nonspecific cross-reacting antigen.
SOURCE      Human colon tumor cell line SW403, cDNA to mRNA, clone 9.
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 2527)
  AUTHORS   Neumaier,M., Zimmermann,W., Shively,L., Hinoda,Y., Riggs,A.D. and
            Shively,J.E.
  TITLE     Characterization of a cDNA clone for the nonspecific crossreacting
            antigen (NCA) and a comparison of NCA and carcinoembryonic antigen
            (CEA)
  JOURNAL   J. Biol. Chem. 263, 3202-3207 (1988)
  STANDARD  simple staff_entry
COMMENT     Draft entry and computer-readable sequence for [1] kindly provided
            by M.Neumaier, 05-FEB-1988.
FEATURES             Location/Qualifiers
     mRNA            <1..2527
                     /note="NCA mRNA"
     CDS             47..1081
                     /note="nonspecific crossreacting antigen precursor"
                     /gene="NCA"
                     /codon_start=1
                     /translation="MGPPSAPPCRLHVPWKEVLLTASLLTFWNPPTTAKLTIESTPFN
                     VAEGKEVLLLAHNLPQNRIGYSWYKGERVDGNSLIVGYVIGTQQATPGPAYSGRETIY
                     PNASLLIQNVTQNDTGFYTLQVIKSDLVNEEATGQFHVYPELPKPSISSNNSNPVEDK
                     DAVAFTCEPEVQNTTYLWWVNGQSLPVSPRLQLSNGNMTLTLLSVKRNDAGSYECEIQ
                     NPASANRSDPVTLNVLYGPDGPTISPSKANYRPGENLNLSCHAASNPPAQYSWFINGT
                     FQQSTQELFIPNITVNNSGSYMCQAHNSATGLNRTTVTMITVSGSAPVLSAVATVGIT
                     IGVLARVALI"
     sig_peptide     47..148
                     /note="nonspecific crossreacting antigen signal peptide"
                     /codon_start=1
     mat_peptide     149..1078
                     /note="nonspecific crossreacting antigen"
                     /codon_start=1
BASE COUNT      731 a    648 c    505 g    643 t
ORIGIN      1137 bp upstream of EcoRI site.
        1 ctcaagctcc tctacaaaga ggtggacaga gaagacagca gagaccatgg gacccccctc
       61 agcccctccc tgcagattgc atgtcccctg gaaggaggtc ctgctcacag cctcacttct
      121 aaccttctgg aacccaccca ccactgccaa gctcactatt gaatccacgc cattcaatgt
      181 cgcagagggg aaggaggttc ttctactcgc ccacaacctg ccccagaatc gtattggtta
      241 cagctggtac aaaggcgaaa gagtggatgg caacagtcta attgtaggat atgtaatagg
      301 aactcaacaa gctaccccag ggcccgcata cagtggtcga gagacaatat accccaatgc
      361 atccctgctg atccagaacg tcacccagaa tgacacagga ttctataccc tacaagtcat
      421 aaagtcagat cttgtgaatg aagaagcaac cggacagttc catgtatacc cggagctgcc
      481 caagccctcc atctccagca acaactccaa ccccgtggag gacaaggatg ctgtggcctt
      541 cacctgtgaa cctgaggttc agaacacaac ctacctgtgg tgggtaaatg gtcagagcct
      601 cccggtcagt cccaggctgc agctgtccaa tggcaacatg accctcactc tactcagcgt
      661 caaaaggaac gatgcaggat cctatgaatg tgaaatacag aacccagcga gtgccaaccg
      721 cagtgaccca gtcaccctga atgtcctcta tggcccagat ggccccacca tttccccctc
      781 aaaggccaat taccgtccag gggaaaatct gaacctctcc tgccacgcag cctctaaccc
      841 acctgcacag tactcttggt ttatcaatgg gacgttccag caatccacac aagagctctt
      901 tatccccaac atcactgtga ataatagcgg atcctatatg tgccaagccc ataactcagc
      961 cactggcctc aataggacca cagtcacgat gatcacagtc tctggaagtg ctcctgtcct
     1021 ctcagctgtg gccaccgtcg gcatcacgat tggagtgctg gccagggtcg ctctgatata
     1081 gcagccctgg tgtattttcg atatttcagg aagactggca gattggacca gaccctgaat
     1141 tcttctagct cctccaatcc cattttatcc atggaaccac taaaaacaag gtctgctctg
     1201 ctcctgaagc cctatatgct ggagatggac aactcaatga aaatttaaag ggaaaaccct
     1261 caggcctgag gtgtgtgcca ctcagagact tcacctaact agagacaggc aaactgcaaa
     1321 ccatggtgag aaattgacga cttcacacta tggacagctt ttcccaagat gtcaaaacaa
     1381 gactcctcat catgataagg ctcttacccc cttttaattt gtccttgctt atgcctgcct
     1441 ctttcgcttg gcaggatgat gctgtcatta gtattcacaa gaagtagctt cagagggtaa
     1501 cttaacagag tatcagattc tatcttgtca atcccaacgt tttacataaa ataagagatc
     1561 ctttagtgca cccagtgact gacattagca gcatctttaa cacagccgtg tgttcaaatg
     1621 tacagtggtc cttttcagag ttggacttct agactcacct gttctcactc cctgttttaa
     1681 tttcaaccca gccatgcaat gccaaataat agaattgctc cctaccagct gaacagggag
     1741 gagtctgtgc agtttctgac acttgttgtt gaacatggct aaatacaatg ggtatcgctg
     1801 agactaagtt gtagaaatta acaaatgtgc tgctggtaaa atggctacac tcatctgact
     1861 cattctttat tctattttag ttggtttgta tcttgcctaa ggtgcgtagt ccaactcttg
     1921 gtattaccct cctaatagtc atactagtag tcatactccc tggtgtagtg tattctctaa
     1981 aagctttaaa tgtctgcatg cagccagcca tcaaatagtg aatggtctct ctttggctgg
     2041 aattacaaaa ctcagagaaa tgtgtcatca ggagaacatc ataacccatg aaggataaaa
     2101 gccccaaatg gtggtaactg ataatagcac taatgcttaa gatttggtca cactctctca
     2161 cctaggtgag cgcattgagc cagtggtgct aaatgctaca tactccaact gaaatgttaa
     2221 ggaagaagat agatccaatt aaaaaaaatt aaaaccaatt taaaaaaaaa aagaacacag
     2281 gagattccag tctacttgag ttagcataat acagaagtcc cctctacttt aacttttaca
     2341 aaaaagtaac ctgaactaat ctgatgttaa ccaatgtatt tatttctgtg gttctgtttc
     2401 cttgttccaa tttgacaaaa cccactgttc ttgtattgta ttgccagggg ggagctatca
     2461 ctgtacttgt agagtggtgc tgctttaatt cataaatcac aaataaaagc caattagctc
     2521 tataact
//