From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Escherichia coli lipoic acid synthetase (lip) gene, complete cds.
Date: 22 Jul 92 10:01:32 GMT
Distribution: bionet

LOCUS       ECOLIP       1445 bp ds-DNA             BCT       22-JUL-1992
DEFINITION  Escherichia coli lipoic acid synthetase (lip) gene, complete cds.
ACCESSION   M82805
KEYWORDS    lip gene; lipoic acid synthetase.
SOURCE      Escherichia coli (strain K-12) DNA.
  ORGANISM  Escherichia coli
            Prokaryotae; Gram-negative facultatively anaerobic rods;
            Enterobacteriaceae.
REFERENCE   1  (bases 1 to 1445)
  AUTHORS   Hayden,M.A., Huang,I., Bussiere,D.E. and Ashley,G.W.
  TITLE     The biosynthesis of lipoic acid: Cloning of LIP, a lipoate
            biosynthetic locus of Escherichia coli
  JOURNAL   J. Biol. Chem. 267, 9512-9515 (1992)
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     CDS             153..998
                     /function="Synthesis of alpha-(+)-lipoic acid"
                     /standard_name="LIP-SYN"
                     /product="lipoic acid synthetase"
                     /gene="lip"
                     /note="putative"
                     /codon_start=1
                     /translation="MKIKLPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGT
                     ATFMILGAICTRRCPFCDVAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLR
                     DGGAQHFADCITAIREKSPQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPR
                     IYRQVRPGADYNWSLKLLERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVT
                     MLTLGQYLQPSRHHLPVQRYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAK
                     GMEVK"
                     /citation=[1]
     terminator      1029..1058
                     /standard_name="rho-independent transcription terminator"
                     /gene="lip"
                     /note="putative"
                     /citation=[1]
     CDS             complement(1099..1305)
                     /note="ORF"
                     /note="putative"
                     /codon_start=1
                     /translation="SMGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAM
                     IDDDAAAKKGADVDLQAEKLSHKE"
                     /citation=[1]
                     /partial
     RBS             complement(1308..1314)
                     /note="ribosomal binding site for small URF"
                     /note="putative"
                     /citation=[1]
     -10_signal      complement(1381..1387)
                     /note="putative"
                     /citation=[1]
     -35_signal      complement(1406..1411)
                     /note="putative"
                     /citation=[1]
BASE COUNT      370 a    385 c    353 g    337 t
ORIGIN
        1 ccttcgtaat tcgcaactgg aacacgcacg ctatgagtaa acccattgtg atggaacgcg
       61 gtgttaaata ccgcgatgcc gataagatgg cccttatccc ggttaaaaac gtggcaacag
      121 agcgcgaagc cctgctgcgc aagccggaat ggatgaaaat caagcttcca gcggactcta
      181 cacgtatcca gggcatcaaa gccgcaatgc gcaaaaatgg cctgcattct gtctgcgagg
      241 aagcctcctg ccctaacctg gcggaatgct tcaaccacgg cacagcaacg tttatgatcc
      301 tcggcgctat ttgtacccgc cgttgtccgt tctgtgatgt tgcccacggt cgcccggtag
      361 ctcctgatgc caatgaacca gtgaaactgg cgcagaccat tgccgatatg gcgctgcgtt
      421 atgtggttat cacctccgtt gaccgtgatg acctgcgcga tggcggtgcc cagcactttg
      481 cggattgcat tactgccatt cgggaaaaaa gcccgcaaat caaaattgaa actctggtgc
      541 cggatttccg cggtcgtatg gatcgtgctc tggatattct gactgcaacg ccaccagatg
      601 tgttcaacca taacctggaa aacgtaccgc gtatttaccg tcaggtacgg cctggtgcag
      661 attacaactg gtcgctgaag ctgctggaac gctttaaaga agcgcatccg gaaatcccga
      721 ccaagtctgg tctgatggtg ggactgggtg aaaccaatga agaaattatt gaggtaatgc
      781 gcgacctgcg ccgtcatggt gtgacgatgt taacgctggg gcaatatttg cagccaagcc
      841 gccatcacct gccggttcaa cgttacgtta gcccggatga gttcgacgaa atgaaagccg
      901 aagcgctggc gatgggcttt acccatgctg catgcggtcc gtttgtccgc tcttcttacc
      961 acgccgattt gcaggcgaaa gggatggaag ttaagtaatc ctgtaataat tacttacatc
     1021 tgtctgataa aaaacccgct ttttgaagcg ggttttttgt atcgaacaag atattgaggg
     1081 agcgtcctgc tcgccacgtc actctttatg agagagcttt tcagcctgaa gatcaacgtc
     1141 tgcgcctttt ttcgccgcag catcgtcatc aatcatcgcc ttcttgaacc ctttaatggc
     1201 cgctccaagg tctccgccca gcgtacgtaa cttcttagtc ccaaacagca gaacgaccag
     1261 cgccgcaact accagcagtt tggtaatact aatctcaccc atagatacct tcttgacata
     1321 taaccggcaa cagaacgcgc cttaaaaacc agagatatta acggtcattc gacgcgcgca
     1381 cacaatagca atctgtaaca aggtgaatca agcgccgagt aaaaaaacat cataataatt
     1441 gcggc
//