From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Corn 22 kDa zein protein gene, complete cds.
Date: 31 Dec 92 01:39:10 GMT
Distribution: bionet
Checksum: 10985 5

LOCUS       MZEZEINA     2500 bp ds-DNA             PLN       17-DEC-1992
DEFINITION  Corn 22 kDa zein protein gene, complete cds.
ACCESSION   M86591
KEYWORDS    22 kDa zein protein; zein protein.
SOURCE      Zea mays (strain W22) DNA.
  ORGANISM  Zea mays
            Eukaryota; Plantae; Embryobionta; Magnoliophyta; Liliopsida;
            Commelinidae; Cyperales; Poaceae.
REFERENCE   1  (bases 1 to 2500)
  AUTHORS   Schmidt,R.J., Ketudat,M., Aukerman,M.J. and Hoschek,G.
  TITLE     Opaque-2 is a transcriptional activator that recognizes a specific
            sequence in 22-kDa zein genes
  JOURNAL   Plant Cell 4, 689-700 (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     protein_bind    769..783
                     /bound_moiety="prolamin box binding protein"
     protein_bind    804..813
                     /function="regulation of 22 k-Da zein gene expression"
                     /bound_moiety="opaque-2 protein"
     CDS             1098..1904
                     /product="22 kDa zein protein"
                     /codon_start=1
                     /translation="MATKILSLFALLALFVSATNAFIIPQCSLAPSAIIPQFLPPVTS
                     MGFEHPAVQAYRLQQALAASVLQQPIDQLQQQSLAHLTIQTIATQQQQQQQILPALSQ
                     LAVCNPVANLQHQFVASNPLALANIVAYQQQQQLQQFLPVLSQLAMVNPAAYLQQQQL
                     ISSSPLAVVNAPTYLQQQLLQQIVPALTQLAVANPAAYLQQLLPFNQLTVSNSAAYLQ
                     QRQQLLNPLAVANPLVAAFLQQQQLLPYNQFSLMNPALKWQQPIVGGAIF"
BASE COUNT      773 a    546 c    414 g    767 t
ORIGIN      
        1 ggatccgatc cggcgcagat ccactataat gcatagtggg tgatgcatgc ctaatggtgg
       61 ttgtttagtg ttagtttggt catgttggaa ctaagcaatt gtcgatgcca tacactagaa
      121 gtgaagaatg ttgttagcgt taggagttga cgtgtcaatg gggaccgata cccgttcaac
      181 catggagaat ttctctataa gatttagggt atgggaagat tttaatttcc atttgctgaa
      241 gttaggtata catctatttg aaccaacaat tacagttcaa cagaaactcc ttccgctcta
      301 gtttgttgtg tgtcctacct ttttcctaaa ctagtttttc ctaaattgac atactctcta
      361 gaaaatctat ccaaattttg aagatcaaat ctgttccatt gaattattca tgacatatat
      421 cttggtattg ctttttgtca tcagaatata ttttgaactc agtaaaagtt atacacgatt
      481 tttttagaca atactaaagc aagatgtaat taagaacact tgaggtgtgc gccaattgcc
      541 acatttagat ataatcattc aaattttggt catcatatgt gtgtgcattg atctatgcat
      601 tctaggattt caattagtct caatcttgta gtatttgttc cttcattgtc aaatcacttc
      661 tcatctaact actatgcttg tttaaccatc agaacataac tacaacaata tccatttata
      721 aaggttttga tagcaaactt tacatattca tatcatgtta aggttgtcac atgtgtaaag
      781 gtgaagagat catgcatgtc attccacgta gatgaaaaga attcctatat aaatgacacc
      841 ttttcttgta ggtagtggaa agtatctttc cagcaaagac catagtaatc cgataaagtt
      901 gataactaaa tgtcaaaata gagtagatgc catatcatct ataccttatc tgttgtttgg
      961 aaaagactaa tccaaaaaat atatatgaga tctcacttgt ataaatagct cccaaatcag
     1021 tagttaacca tcacccataa tattttgagc attcagaaac acacctagcg aagcgcacta
     1081 gcaacgacct aacaacaatg gctaccaaga tattatccct ctttgcgctt cttgcccttt
     1141 ttgtgagcgc aacaaatgcg ttcattattc cacaatgctc acttgctcct agtgccatta
     1201 ttccacagtt cctccctcca gttacttcaa tgggcttcga acacccagct gtgcaagcct
     1261 acaggctaca acaagcgctt gcggcgagcg tcttacaaca accaattgac caattacaac
     1321 aacaatcctt ggcacatcta accatacaaa ccatcgcaac gcaacagcaa cagcaacaac
     1381 aaattctacc agcactgagc caactagctg tgtgtaaccc tgtcgctaac ttgcaacatc
     1441 agttcgttgc atccaaccca cttgctctgg caaacatagt tgcataccaa caacaacaac
     1501 aattgcaaca gtttctacca gtgctcagtc aactagccat ggtgaaccct gccgcctacc
     1561 tacaacagca acaactgatt tcatctagcc ctctcgctgt ggttaatgca cctacatacc
     1621 tgcaacaaca gttgctgcaa cagattgtac cagctctgac tcagctagct gtggcaaacc
     1681 ctgctgccta cttgcaacag ctgcttccat tcaaccaact gactgtgtcg aactctgctg
     1741 cgtacctaca acagcgacaa cagttactta atccactagc ggtggctaac ccattggtcg
     1801 ctgccttcct acagcagcaa caattgctgc catacaacca gttctctttg atgaaccctg
     1861 ccttgaagtg gcagcaaccc atcgttggag gtgccatctt ttagattaca tatgagatgt
     1921 actcgataat ggtgccctca tagcggcatg tgtttcctag aaataatcaa tatattgatt
     1981 gagatttatc tcgatatatt tctaaactat gttcatcata taaataattg ataattgaaa
     2041 acatcaaatc gtaattataa acttatgctt ggtcgataca taaacaataa aatattactt
     2101 catcatccca atgatgtcct agcccaacct cttgaatgtt atagtttggg tgtgtgcggg
     2161 tgtatttata agatagatgt gactatttgc gctttttgtt gagtacatac atatatggta
     2221 tgttgatttg atatagtgat ggacacatgc tttgaccatg aatattcaaa tcggttgtac
     2281 ttgcacgaag caaaacataa tattagttta ggagtaaact tataactgcg tccaaacatg
     2341 ctcacacaaa ttcataccac attataattt tttggtaagt attccacaca tattttttta
     2401 caagaaccca aattttgcac acaaatgtac cattctagac atgtataatt ctttgaagcc
     2461 cgtgaactta acagcaccaa ggtcattaaa tcaattggac
//