From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: acvA=acv synthetase [Aspergillus nidulans, Genomic, 10260 nt,
Date: 29 Dec 92 01:28:17 GMT
Distribution: bionet
Checksum: 31620 19

LOCUS       M132910S1   10260 bp    DNA             NLM       08-DEC-1992
DEFINITION  acvA=acv synthetase [Aspergillus nidulans, Genomic, 10260 nt,
            segment 1 of 2] 
ACCESSION   S40212 X54853
KEYWORDS    .
SEGMENT     1 of 2
SOURCE      Aspergillus nidulans
  ORGANISM  Aspergillus nidulans
            Unclassified.
REFERENCE   1  (bases 1 to 10260)
  AUTHORS   MacCabe,A.P., van Liempt,H., Palissa,H., Unkles,S.E., Riach,M.B.,
            Pfeifer,E., von Dohren,H. and Kinghorn,J.R.
  TITLE     Delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine synthetase from
            Aspergillus nidulans. Molecular characterization of the acvA gene
            encoding the first enzyme of the penicillin biosynthetic pathway.
  JOURNAL   J. Biol. Chem. 266, 12646-12654 (1991)
  STANDARD  full automatic
COMMENT     This entry [NCBI gibbsq 40212] was created by the journal scanning
            component of NCBI/GenBank at the National Library of Medicine.
            sixty bases from 9386 to 9445 are missing from the figure.
            This sequence comes from Fig. 2.  The authors begin their numbering
            at -875.  Zero is not present in the numbering.
FEATURES             Location/Qualifiers
     CDS             876..10259
                     /note="For the protein sequence (NCBI gibbsq 40255):
                     Method: conceptual translation with partial peptide
                     sequencing.  20 a.a. gap from 3129 to 3148 are missing.
                     This sequence comes from Fig. 2."
                     /gene="acvA"
                     /product="acv synthetase"
                     /codon_start=1
                     /translation="MSPPGLLSEDGPGYSGGYADPTVPKVNWKQSNGKSAGGNGDVDA
                     GNGNIDPSKSGVGVQVCFAGGLEGWKAGISKITERCDLSSIATNSTKYQLAVTGFSDG
                     PDDYNEYSVPFPSEVLVAMEEMCLARDISMRSVIQFAVHYVLKGFGGGSHTVAASIDV
                     GDDPNNIATSYTITPSIVCHESRQGQTVMQEIQSMEKLNQLRKQEMHPGEAGLSLIRM
                     GLFDILVIFADANKCEGLIAGLPLAVMVCEGGGRLQVRIHFSGSLFRQKTLVDIAEAL
                     NVLFAKAASGGATPVRDLELLSAEQKQQLEEWNKTDGEYPECKRLNHLIEEATQLHED
                     KVAIVYKRRQLTYGELNAQANCFAHYLRSIGILPEQLVALFLEKSENLIVTILGIWKS
                     GAAYVPIDPTYPDERVRFVLEDTQAKVIIASNHLAERLQSEVISDRELSIIRLEHCLS
                     AIDQQPSTFPRANLRDPSLTSKQLAYVTYTSGTTGFPKGILKQHTNVVNSITDLSARY
                     GVTGDHHEAILLFSAYVFEPFVRQMLMALVNGHLLAMVDDAEKYDAEKLIPFIREHKI
                     TYLNGTASVLQEYDFSSCPSLKRLILVGENLTESRYLALRRHFKNCILNEYGFTESAF
                     VTALNVFEPGSARNNTSLGRPVRNVKCYILNKSLKRVPIGATGELHIGGLGISKGYLN
                     RPDLTPQRFIPNPFQTDHEKELGLNQLMYKTGDLARWLPNGEIEYLGRADFQIKLRGI
                     RIEPGEIESTLAGYPGVRTSLVVSKRLRHGEKETTNEHLVGYYVGDNTSVSETALLQF
                     LELKLPRYMIPTRLVRVSQIPVTVNGKADLRALPSVDLIQPKVSSCELTDEVEIALGK
                     IWADVLGAHHLSISRKDNFFRLGGHSITCIQLIARIRQQLGVIISIEDVFSSRTLERM
                     AELLRSKESNGTPDERARPQLKTVAGEVANANVYLANSLQQGFVYQFLKNMGRSEAYV
                     MQSVLRYDVNINPDLFKKAWKQVQHMLPTLRLRFQWGQDVLQVIDEDQPLNWWFLHLA
                     DDSALPEEQKLLELQRRDLAEPYDLAAGSLFRIYLIEHSSTRFSCLFSCHHAILDGWS
                     LPLLFRKTHGTYLHLLHGHSLRTLEDPYRQSQQYLQDHREDHLRYWAGIVNQIEERCD
                     MNALLNERSRYKIQLADYDKVEDQQQLTLTVPDASWLSKLRQTCSAQGITLHSILQFV
                     WHAVLHAYGGGTHTVTGTTISGRNLPVSGIERSVGLYINTLPLVINQLAYKNKTVLEA
                     IRDVQAIVNGMNSRGNVELGRLQKNELKHGLFDSLFVLENYPILDKSEEMRQKSELKY
                     TIEGNIEKLDYPLAVIAREVDLTGGFTFTICYARELFDEIVISELLQMVRDTLLQVAK
                     HLDDPVRSLEYLSSAQMAQLDAWNATDAEFPDTTLHAMFEKEAAQKPDKVAVVYEQRS
                     LTYRQLNERANRMAHQLKSDISPKPNSIIALVVDKSEHMIATILAVWKTGGAYVPIDP
                     EYPDDRIRYILEDTSAIAVISDACYLSRIQELAGESVRLYRSDISTQTDGNWSVSNPA
                     PSSTSTDLAYIIYTSGTTGKPKGVMVEHHGVVNLQISLSKTFGLRDTDDEVILSFSNY
                     VFDHFVEQMTDAILNGQTLVMLNDAMRSDKERLYQYIETNRVTYLSGTPSVISMYEFS
                     RFKDHLRRVDCVGEAFSQPVFDQIRDTFQGLIINGYGPTEISITTHKRLYPFPERRTD
                     KSIGQQIGNSTSYVLNADMKRVPIGAVGELYLGGEGVARGYHNRPEVTAERFLRNPFQ
                     TDSERQNGRNSRLYRTGDLVRWIPGSNGEIEYLGRNDFQVKIRGLRIELGEIEAVMSS
                     HPDIKQSVVIAKSGKEGDQKFLVGYFVASSPLSPGAIRRFMQSRLPGYMIPSSFIPIS
                     SLPVTPSGKLDTKALPTAEEKGAMNVLAPRNEIESILCGISAGLLDISAQTIGSDSDF
                     FTLGGDSLKSTKLSFKIHEVFGRTISVSALFRHRTIESLAHLIMNNVGDIQEITPVDY
                     DNRRKIAVSPAQERLLFIHELEGGGNAYNIDAAFELPPYIDQSRVEEALYTILSRHEA
                     LRTFLLRDQATGTFYQKILTTDEAKCMLIIEKSAVSTIDQIDSIVGRLSQHIFRLDSE
                     LPWLAHIVTHKTGNLYLTLSFHHTCFDAWSLKIFERELRVFCASKQKGGNMPILPMPQ
                     VQYKEYAEHHRRRLGKNQIQKLSDFWLQRLDGLEPLQLLPDYPRPAQFNYDGGDLSVI
                     LDGVVLETLRGIAKDHGVTLYAVLLAVYCLMLSTYTHQVDIAVGVPISHRTHPLFQSI
                     VGFFVNMVVVRVDVKDFAVHDLIRRVMKPLVDAQLHQDMPFQDVTKLLRVDNDASRHP
                     LVQTVFNFESDMDKEFETTPSIQDTATIAPYQSVQRIKSVAKFDLNATATESGSALKI
                     NFNYATSLFRKETIQGFLETYRHLLLQLSYLGSQGLKEDTKLLLVRPEEMSGPHLPLA
                     GLSNGAETLEAISLSRAFEFEAFRVPDRAAVVQGDKSLSYTELNKRANQLARYIQSVA
                     HLRPDDKVLLILDKSIDMIICILAIWKTGSAYVPLDPSYPKERVQCISEVVQAKILIT
                     ESRYASAWGSQTSTILAIDSPKVSNMVNNQATHNLPNIAGIKNLAYIIFTSGTSGKPK
                     GVLVEQGGVLHLRDALRKRYFGIECNEYHAVLFLSNYVFDFSIEQLVLSIMSGHKLII
                     PEGEFVADDEFYITANGQRLSYLSGTPSLLQQIDLARLNHLQVVTAAGEQLHAAQFNK
                     LRSGFRGPIYNAYGITETTVYNIVSEFSAQSQFENALRELLPGTRAYLLNHATQPVPM
                     NAVGELYLAGDCVARGYLNQPVLTGDRFIQNPFQTEQDIACGSYPRLYRTGDLFRCRL
                     DRQHQPYLEYLGRADLQVKIRGYRIEPSEVQNVLASCPGVRECAVVAKYENTDAYSRI
                     AKFLVGYYTPDTETVSDSSILAHMKSKLPAYMVPKYLCRLEGGLPVTINGKLDVRKLP
                     DIGNPQHQISYNPPRDVLEADLCRLWASALGTERCGIDDDLFRLGGDSITALHLAAQI
                     HHQIGRKVTVRDIFDHPTIRGIHDNVMVKLVPHNVPQFQAEQQTVLGDAPLLPIQIWF
                     LSKSLQHPSH"
BASE COUNT     2652 a   2563 c   2581 g   2464 t
ORIGIN      
        1 cattatgaat gagggcaagc agttgagact gatgaagaag cggtggtaac ggaaggatcc
       61 ccaaggcttg gactggtttg tgtaggttaa tgggtaggaa ttgccggcga agaacagacg
      121 tttctgcgaa tttccatcag ggtttattta ttttcccgtc tgcgcgcaag tctccaagca
      181 aataagcgac cgggtaggac tggcttggac atcgagccga ccacactcgt cgcaaaagta
      241 cgaggggctg tagacaagta tagtcatggc ggccttcgtc gggtgacgcc taaaaccatt
      301 accctgcaga aattgaatca gcgtaagaca ctgcagcaat agccccgcca ggaatagagg
      361 gcttggctcg taccacaatt tgatgagtag gaatggacta cgtcagccct aagcgagaga
      421 atattgggaa atgatcataa cgaagcttgt tacccttttg cgattggccc tttcccccaa
      481 tatagggcca atattacggt gcagagccag ttgggacaat ctgcaggaga cgtgaaacat
      541 catctcctgc gtaccctatg gcactagtac ctagcatggt ctgatgctag gccgtgacgg
      601 tgtcgcttgg ccgtccccac ggcacggctt gatggtacga tgctgcggta ctggattgcc
      661 gggtagcgaa gtcagtccct aatcttgtga aggcctcttg gcagtgcttc gactggaatc
      721 ggccaggcgg agctgtagta cccctcgtct aggaggggag ctcggtttag cactgtgggc
      781 ggacttttcg cggtgtcctc acttctaggc cgtcaactga cttctcaagc gatatcccct
      841 caggcttttg ccctccgtga aataagagaa tcaaaatgag ccctcccggg ctattgagcg
      901 aagacggccc tggctacagt ggcggctatg cagaccctac ggtgccaaag gttaattgga
      961 agcagtccaa tgggaaaagc gccgggggca atggcgacgt tgatgcaggc aatggcaaca
     1021 ttgaccctag caaatcgggt gttggtgtcc aagtgtgttt tgcaggaggg cttgaaggtt
     1081 ggaaagccgg catcagcaaa ataactgaac gttgtgatct gagcagtatt gcaacaaact
     1141 cgacgaaata ccagcttgcg gtaaccgggt tcagtgatgg accggatgac tacaatgagt
     1201 actcggttcc ttttccctca gaagtacttg tcgcgatgga agaaatgtgt cttgcacgag
     1261 atattagtat gaggtctgtg atccagtttg cagtgcatta tgtgttgaaa gggttcggtg
     1321 gtggctcaca tactgttgct gcgtcgatcg atgtgggtga cgaccccaat aacatagcga
     1381 catcatacac tattacaccc tcaattgtct gccatgagag cagacaagga cagaccgtga
     1441 tgcaggagat tcagagtatg gaaaagttaa accaattgag gaagcaagaa atgcatccgg
     1501 gggaggctgg attaagtctc atcagaatgg ggttattcga cattctggtt atcttcgcag
     1561 atgcaaacaa gtgtgagggt ctaattgctg gcttgcctct agcagtaatg gtgtgcgaag
     1621 gaggtggaag acttcaggtt agaatacact tctcagggtc cctttttcga cagaagacgt
     1681 tagtggatat cgccgaagcc ctgaacgtct tgttcgctaa ggctgcgtcg gggggagcga
     1741 cgccggtccg agatcttgaa cttctttctg cagagcaaaa gcagcagtta gaagagtgga
     1801 acaagacgga tggagagtac cctgaatgca aaagactcaa tcaccttatt gaggaggcga
     1861 cacagctgca tgaagacaaa gttgccatcg tgtacaaacg tcgccagctt acatacggcg
     1921 aattgaacgc gcaggccaac tgtttcgcgc actatctgcg gtccatcggg atcttacctg
     1981 agcagctggt ggctttattt ctcgagaaga gcgagaacct tatcgtgact atattgggta
     2041 tctggaagtc cggcgccgca tatgtgccca ttgacccaac ctaccctgat gaacgagtcc
     2101 gctttgtgct tgaagacact caggcaaaag tcatcattgc gagcaaccac cttgcagaga
     2161 gacttcaaag cgaggtcatc agcgacaggg agctctccat tattcgtcta gagcattgct
     2221 tgagcgccat tgatcagcag ccatcgacat tcccgagagc caatttgcgc gacccatctc
     2281 tgaccagcaa gcagcttgcc tacgttacct atacatcggg gaccacgggt tttccgaagg
     2341 gcattctcaa gcaacacact aacgtggtga acagcatcac tgacctttca gctcggtatg
     2401 gggtgacagg ggaccatcat gaagccatcc tgctcttttc agcgtatgtg tttgagccct
     2461 tcgtgcggca gatgctcatg gcactagtga atggccattt gctcgctatg gtcgatgatg
     2521 ctgagaagta tgatgccgaa aagttgatac cattcattcg tgagcacaag atcacgtacc
     2581 tcaacggcac tgcctccgtc ctgcaggaat acgacttctc ctcttgccca tctctaaagc
     2641 gtttgatctt ggtcggtgag aacttgactg aatctcggta tctggcacta cgtagacatt
     2701 tcaagaattg catattgaac gagtatggct tcacagaatc agcctttgtg acggcgctca
     2761 atgttttcga accaggctcg gcgcgcaata acacgagtct tgggaggccg gtgcgcaacg
     2821 tcaagtgtta tatcctcaac aagtctctca agcgagtgcc tattggtgcc actggtgaat
     2881 tacacattgg cgggctgggt atatccaagg gctaccttaa ccgtcccgac cttacgccgc
     2941 aacgcttcat tcccaaccca ttccaaacgg accatgagaa ggagctcgga ttaaaccagc
     3001 tgatgtacaa gaccggggat ctcgcccgtt ggcttccaaa cggtgagatc gagtacctcg
     3061 gccgcgcgga cttccaaatc aagctgcgag ggatccgtat cgagcccggc gagatagagt
     3121 ccactctggc gggttaccct ggggtacgaa ccagcctagt cgtctctaaa aggttgcggc
     3181 atggcgaaaa ggagactacc aacgagcatc tggtaggcta ttatgtgggc gataatacct
     3241 ctgtctctga aacggctctc ttgcaatttc tggagctgaa gctgccccga tacatgattc
     3301 cgacacgact tgtgcgcgtg tctcaaatcc cagtgactgt taatggaaag gcagacctcc
     3361 gtgccctacc ttctgtcgac cttattcaac ccaaagtgtc ctcttgcgag ctcacggatg
     3421 aggtggaaat agctttgggg aagatatggg cagatgttct cggagcccat cacctgtcga
     3481 tatcccgtaa agacaacttc tttcgtcttg gagggcacag catcacatgc atccagctca
     3541 tcgcacgtat tcgccagcag cttggtgtaa ttatttccat tgaggacgtt ttctcatccc
     3601 ggacactgga gcgtatggct gagcttctgc gaagcaaaga gtccaacgga actccggatg
     3661 agagggctag gcctcaacta aaaaccgtgg cgggagaagt tgcaaatgct aatgtctatc
     3721 ttgctaacag tctccagcaa ggcttcgttt atcagttcct gaaaaatatg ggccgatcag
     3781 aggcttatgt gatgcaatcc gtgctgcgat acgatgtcaa tatcaatcct gatctattta
     3841 aaaaagcctg gaagcaggta caacacatgc ttccaacact gaggctccga tttcaatggg
     3901 gacaggatgt tttgcaggtg attgacgagg accagccgct gaactggtgg ttcttacacc
     3961 ttgccgacga ttcagccctg cccgaggagc agaaactact agagttacag cgcagggacc
     4021 tggctgagcc atacgaccta gcagccggaa gcctgttccg catttatctg atcgagcata
     4081 gctcaactcg gttttcgtgc ttgttcagct gtcatcacgc aatccttgat ggatggagcc
     4141 tgccgcttct tttcaggaag actcatggaa cttatctgca tctcctgcac ggacattctc
     4201 tcaggactct ggaagaccct tacaggcagt ctcagcagta tctccaagat catcgcgaag
     4261 atcatctcag gtactgggct ggtatcgtga atcagattga agagcgttgt gacatgaacg
     4321 ctttgctgaa cgaacgcagt cggtacaaga ttcaactggc ggactatgac aaagtggagg
     4381 atcaacaaca attaacttta acagtccctg atgcttcctg gctaagcaaa ttgcgccaaa
     4441 catgctctgc gcaaggcatt acattgcact ctattctgca gtttgtttgg cacgcggtat
     4501 tgcatgctta cggtggcggt actcatactg tcactggcac tactatctca gggaggaacc
     4561 tgcctgtgag tgggatcgaa cgatctgtgg gtctctacat aaatacgctc ccactggtaa
     4621 ttaatcagtt ggcctataag aataaaaccg tcttggaggc tatccgtgat gtgcaggcca
     4681 ttgtaaatgg catgaacagc cggggaaatg tggaacttgg ccgtctacag aaaaacgagc
     4741 tgaagcatgg gttatttgac tcgctatttg tgctggagaa ttatccaata ctggacaagt
     4801 ccgaggagat gcggcagaag agtgaattga agtataccat cgaaggcaat attgaaaagc
     4861 tcgactatcc ccttgctgtt atcgcgcgcg aggtcgacct aactggggga ttcaccttca
     4921 ccatctgcta cgctcgagag cttttcgatg agattgttat atctgagttg ctccaaatgg
     4981 tccgggacac gctcctgcaa gtcgcgaagc atttagatga ccccgtccgc agcctagagt
     5041 atctgtcatc agcgcaaatg gctcaacttg acgcatggaa tgcgacagac gcggaattcc
     5101 ccgacaccac cctacacgcg atgttcgaaa aagaagcggc ccagaaacca gacaaggtcg
     5161 cggtggtcta tgagcaacgc agcttgacgt atcgtcagct aaatgagcgg gcgaaccgta
     5221 tggcgcacca gctcaaatct gatatcagcc caaagccgaa cagtatcatt gctctggtag
     5281 tggataagag tgagcatatg atagctacca ttctggctgt gtggaagact ggcggtgcct
     5341 atgtaccgat cgaccctgag taccccgacg accgtatccg ctatatccta gaagacacca
     5401 gcgccattgc cgtgatttca gacgcgtgtt acctctcacg aatccaagaa ttagcgggag
     5461 agagtgtccg tctgtatcgg tctgacatct ctactcagac tgacggtaac tggagtgtgt
     5521 cgaatcctgc accgtccagt acgagcacgg atcttgcata tattatctac acttcgggaa
     5581 caactgggaa gccaaagggc gtcatggtgg agcaccacgg agtggtaaat ctgcagatat
     5641 cgctgtctaa aaccttcggg ctgcgcgata ctgatgacga ggtaatcctc tcattctcca
     5701 actacgtctt tgaccatttc gtggaacaga tgacggatgc cattctcaac ggccaaacat
     5761 tagttatgct caacgatgca atgcgcagtg acaaagagcg cctctaccaa tatatcgaaa
     5821 ctaatagggt aacatacctg tctggaaccc catccgttat ttccatgtat gagttcagtc
     5881 gatttaaaga ccacctacgc cgtgtcgact gcgttggaga agcttttagc cagcccgtct
     5941 ttgatcaaat ccgtgacact ttccaagggc tgattatcaa cggctacggt ccaacagaga
     6001 tctccatcac gacacacaag cggctgtacc ctttccctga gcggcgcaca gataagagca
     6061 tcggccagca gattggcaac agtacgagct acgtgctgaa tgcagacatg aaacgcgttc
     6121 caattggggc tgtaggtgag ctctatctgg gtggtgaagg cgtcgcgcga ggatatcata
     6181 accgaccgga agtgactgct gagcgatttt tacgcaatcc gttccaaaca gacagtgaac
     6241 ggcaaaatgg gcgcaacagc cgcttgtaca ggaccggtga cttggtacgc tggatcccag
     6301 gcagtaacgg tgaaattgaa tatttgggac gcaatgactt ccaggtcaag attcgcgggc
     6361 tccgtatcga attgggggag attgaggctg tcatgtcctc acatcctgac attaaacagt
     6421 ctgttgtaat tgcaaagagt ggcaaggaag gagaccagaa gttccttgtt ggttacttcg
     6481 tggctagctc gccattgtct ccgggtgcaa tccggcgctt tatgcaatcc cggcttcctg
     6541 gctatatgat accttcaagt ttcattccta tcagttctct cccagtgact cccagtggaa
     6601 agctggatac aaaggcctta cctacagcag aggagaaagg cgcaatgaac gtgctggctc
     6661 cacgtaatga aatcgagagc atcctgtgcg gtatctcggc agggttgtta gatatatccg
     6721 cccaaacaat tggcagcgac agcgattttt tcaccctcgg aggcgatagt ttgaagagta
     6781 caaagctctc attcaagatt cacgaggtat ttggccgcac aatctccgtc agcgctctgt
     6841 tccgtcaccg aaccatcgag agtctggcac acctaattat gaacaatgtt ggagacatac
     6901 aggagatcac gcctgtggat tatgataaca gacgcaaaat agccgtatct cccgctcaag
     6961 agcgccttct attcattcac gagcttgaag gtggaggcaa tgcatataat atcgatgctg
     7021 cctttgagct acctccatac attgatcaat ctcgagtcga agaggcatta tataccattc
     7081 tttcaagaca cgaagcctta cgaacatttc tgctgcggga ccaggcaact ggcacgttct
     7141 accaaaagat attgactacc gatgaggcca agtgcatgtt gatcattgag aaaagtgcag
     7201 tgagcaccat tgatcaaatt gattccatag tcggacgcct atcgcagcac attttccgtc
     7261 tcgattctga gcttccctgg ttggcgcata ttgtcacgca caaaacgggc aatctttatc
     7321 tgaccctgtc cttccatcac acttgcttcg atgcatggtc attgaagatc ttcgagcggg
     7381 agctccgcgt tttttgcgcg tcaaagcaaa aaggcggcaa catgccaatc ctaccaatgc
     7441 ctcaagtcca gtacaaggag tatgccgagc accatcgtcg acgactaggt aagaatcaga
     7501 ttcaaaaatt atccgacttt tggctgcaaa gactagacgg cctggagccc ctacagctcc
     7561 taccggatta tccgcggcct gcccaattca actacgatgg aggtgacctc tccgtcattc
     7621 tggacggtgt ggttctggaa accctcaggg gcattgcaaa agaccacgga gtaactctgt
     7681 acgcagtgct tctcgctgtt tactgcctga tgctttcgac atatacacac caggtagata
     7741 tcgctgtggg agtccccatc agtcaccgaa cccaccccct gttccagtct attgtcggat
     7801 tcttcgtcaa tatggtagtt gtgagggtcg acgtgaagga ctttgccgtt cacgatctca
     7861 ttcgaagggt aatgaaaccg cttgttgatg cccagttaca tcaggacatg ccattccaag
     7921 acgtgactaa actgctgcgg gtggataacg acgccagccg acatccccta gttcagactg
     7981 tgttcaactt tgaaagtgac atggacaaag aattcgagac gacaccttca atccaagaca
     8041 ctgccacaat cgcaccatac cagtccgttc agaggataaa gtcggttgcg aaatttgatc
     8101 tgaacgcgac agctacagag tcgggctcag ccttaaagat taactttaac tatgccacca
     8161 gcctgttccg gaaagaaacg atccagggct tcttagagac atacaggcat ctcctgttac
     8221 agctctctta tctggggtcc cagggactta aagaagatac aaagctactg ttggtccgcc
     8281 ctgaggagat gagtggtccg catctgccat tagcaggatt atccaatggt gcggaaaccc
     8341 tagaagctat atcactcagt agagcattcg agtttgaagc tttcagggta ccggatagag
     8401 ctgccgtcgt acagggagat aaatcactca gctataccga gctcaataaa cgggcaaacc
     8461 agctagcccg gtacatacaa tccgtggcac accttaggcc ggacgacaag gtgctcctca
     8521 ttctggataa gagcatcgac atgattattt gcatcctcgc aatctggaaa accggtagcg
     8581 catatgtgcc tttggatcca tcatatccca aggagcgtgt ccagtgcatt tcggaggtag
     8641 ttcaagcaaa gattctgatt acagagtcac ggtacgcctc tgcatgggga agccagacgt
     8701 caacaatact tgcaattgac tcgcccaagg tctcgaatat ggtcaataat caggcaactc
     8761 ataacttgcc caacattgcg ggaataaaaa atctggcata tataattttc acatctggca
     8821 cctccggcaa gccaaagggt gttctggtcg aacaaggtgg agttcttcac ttgcgtgatg
     8881 cgcttaggaa gcggtacttt ggcattgaat gcaatgaata ccatgctgtg ctcttcctat
     8941 ccaattacgt gtttgatttc tctatcgagc agttggtctt atcaattatg agcggccaca
     9001 agttgatcat cccggaagga gaattcgttg cggatgatga attctacata acagccaacg
     9061 gtcaacgcct ctcatatttg agcggtacac catccctgtt gcagcaaatt gacctagcac
     9121 gcctcaatca tctacaggtc gtaactgcag ctggtgagca actccatgct gcgcagttta
     9181 ataagttgcg ctccggattc cgcggcccga tctacaacgc atatggaatt acggagacca
     9241 cggtatacaa catagtcagc gagttcagtg cgcaatccca attcgaaaat gctctgcgag
     9301 agctgctacc aggcactagg gcatatcttc ttaaccacgc cactcagcca gttcctatga
     9361 acgcagtcgg agagctgtat ctcgctggtg attgtgtggc ccgtggctat ctcaaccagc
     9421 ctgttctaac aggtgaccgt tttatccaga atccattcca aacagagcaa gatattgctt
     9481 gcggaagcta tcctcggctc tatagaactg gcgacctgtt tcgatgccgg cttgaccgtc
     9541 agcaccagcc atatctagaa tatcttggaa gagctgatct ccaggtcaag ataagaggat
     9601 accgtattga gccgtcagaa gttcagaacg tgcttgcttc ctgtcctggc gttcgagaat
     9661 gtgcagtagt ggccaagtat gagaacaccg atgcttactc caggatagcc aaattcctgg
     9721 tcggatatta tacccctgac accgagacgg tctccgattc aagtatcctc gcccacatga
     9781 aaagcaagct tcccgcatat atggtcccta aatatctatg ccgtctagaa ggtggacttc
     9841 cagtgacaat caacgggaaa cttgacgttc gaaagctgcc tgatatcggc aaccctcaac
     9901 atcaaatatc gtacaacccc ccaagggatg tcctggaggc cgacttgtgt agattatggg
     9961 catcagcact aggaacagag cgatgcggta ttgatgatga tctgtttagg ttaggcggag
    10021 acagtattac tgctttgcat ctcgcagccc aaatccacca ccagatcggc cgaaaggtca
    10081 ctgttcgaga tattttcgac caccctacca ttcgtggtat tcatgacaac gttatggtga
    10141 aactcgttcc acacaatgtt cctcaattcc aagcagagca gcaaacagta ctcggtgatg
    10201 cgcctctgct accgatccaa atttggttct tatcaaaatc gctacagcac ccaagccatt
//