From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: D.pseudoobscura Adh gene for alcohol dehydrogenase
Date: 28 Dec 92 21:27:01 GMT
Distribution: bionet
Checksum: 53873 8

LOCUS       DROADHGN33   3463 bp ds-DNA             INV       01-DEC-1992
DEFINITION  D.pseudoobscura Adh gene for alcohol dehydrogenase
ACCESSION   X62213
KEYWORDS    ADH gene; alcohol dehydrogenase.
SOURCE      Drosophila pseudoobscura DNA.
  ORGANISM  Drosophila pseudoobscura
            Eukaryota; Animalia; Metazoa; Arthropoda; Uniramia; Insecta;
            Pterygota; Neoptera; Holometabola; Diptera; Brachycera;
            Cyclorrhapha; Schizophora; Drosophiloidea; Drosophilidae.
REFERENCE   1  (bases 1 to 3463)
  AUTHORS   Schaeffer,S.W. and Miller,E.L.
  TITLE     Estimates of gene flow in Drosophila pseudoobscura determined from
            nucleotide sequence analysis of the alcohol dehydrogenase region
  JOURNAL   Genetics 132, 471-480 (1992)
  STANDARD  full automatic
COMMENT     *source: strain=GB82; *source: isolate=Gundlach Bundschu Winery,
            Sonoma Valley, *source: California; *source: chromosome=IV section
            88;
               From EMBL    entry DPADHGN33;  dated 16-OCT-1992.
FEATURES             Location/Qualifiers
     mRNA            join(40..119,915..1020,1084..1488,1549..1964)
                     /note="adult mRNA (Adh)"
     mRNA            join(882..1020,1084..1488,1549..1964)
                     /note="larval mRNA (Adh)"
     promoter        8..14
                     /note="adult promoter (Adh)"
     prim_transcript 40..1964
                     /note="adult primary transcript (Adh)"
     exon            40..119
                     /note="adult exon 1a"
     intron          120..914
                     /note="adult intron"
     promoter        846..853
                     /note="larval promoter (Adh)"
     prim_transcript 882..1964
                     /note="larval primary transcript (Adh)"
     exon            882..1020
                     /note="larval exon 1"
     exon            915..1020
                     /note="adult exon 1b"
     intron          1021..1083
                     /number=1
     exon            1084..1488
                     /number=2
     intron          1489..1548
                     /number=2
     exon            1549..1964
                     /number=3
     polyA_signal    1941..1946
     polyA_site      1964
     CDS             join(928..1020,1084..1488,1549..1815)
                     /EC_number="1.1.1.1"
                     /gene="Adh"
                     /product="alcoholdehydrogenase"
                     /codon_start=1
                     /translation="MSLTNKNVVFVAGLGGIGLDTSRELVKRNLKNLVILDRIDNPAA
                     IAELKAINPKVTITFYPYDVTVPVAETTKLLKTIFAQVKTIDVLINGAGILDDHQIER
                     TIAVNYTGLVNTTTAILDFWDKRKGGPGGIICNIGSVTGFNAIYQVPVYSGSKAAVVN
                     FTSSLAKLAPITGVTAYTVNPGITKTTLVHKFNSWLDVEPRVAEKLLEHPTQTSQQCA
                     ENFVKAIELNKNGAIWKLDLGTLEPITWTQHWDSGI"
BASE COUNT      976 a    789 c    793 g    905 t
ORIGIN      
        1 cgtagtgtat ttaaggcact gcacatgtcg aggaccacga ttattgtcta cagtgcagtc
       61 agtagttgtc aggtgcagca ggcggaccag catttcttta aatctactca atcgatcaag
      121 taagtaactg taatccaagt acagtcggcc catcccactc ggttctgccg taactcaaga
      181 aatttcttga tagcgcagtt ccagttgtaa aacgtcgttt aaaaaaacat ctcgttggag
      241 cttgggttga aaaacgttcg agttgggcgt agaaaagttc aaatataaaa tatcaaaaaa
      301 gagaaaatat cttcacaaag cagctgtcca ttttttgtta aagtgataga tcaaaaattc
      361 ggatgacgta cattcctagt gcacttcata caaataattt cctcaattct aattctacaa
      421 gaagcacttt tggcacttgt ccagaaaaaa caatttgttt acacttttcg gttgataaca
      481 gaggcggacc gacggctaag taacataaca gtcgcctgtt aagtaatgcc ctcactctct
      541 gttagatggc cgcgcgtgtc gactccgata gaccagcaga cacgcagtag ttttcctcaa
      601 gaacctgaac tctaaacata gacataattt acgatacctt gagactcaaa taaaaataca
      661 agtatgagtg gtattctatt cctgattaga ctcgcacaca cacacgtacg agtattaaca
      721 taaatttgcc ggcaaataac caaaacaaaa cgaacgcaaa cgatccgaac acgtaatgcg
      781 agagataaga aacagaaaag ctcgacgtga aagaagcttc tggcggaagc tcttgccgaa
      841 ctccctataa atacgagaca gaaaaccaga ataaatataa taacaaactg ttgaattgtc
      901 ccatcgattc ccagatcaaa agacagaatg tctctcacca acaagaacgt cgttttcgtg
      961 gccggtctgg gcggcattgg cctggacacc agccgggagt tggtcaagcg taatctgaag
     1021 gtaagagtga acgaattcca tggaatctat ggaatcctaa atttaaaaat ttattttttt
     1081 tagaacctgg tcatcctgga tcgcattgac aatccggctg ccattgccga actgaaggca
     1141 atcaatccca aggtgaccat caccttctat ccctacgatg tgactgtgcc cgtcgctgag
     1201 accaccaagc tcctgaagac catctttgcc caggtgaaga caatcgatgt cctgatcaac
     1261 ggtgctggca tcctggacga tcatcagatc gagcgcacca ttgccgttaa ctacacgggc
     1321 ctggtcaaca ccaccacagc cattctggac ttctgggaca agcgcaaggg cggcccaggc
     1381 ggcatcattt gcaacattgg ctccgtcacc ggtttcaatg ccatctacca ggtgcccgtt
     1441 tactccggct ccaaggcggc ggtggttaac tttacctcct ccctggcggt aagaaatcct
     1501 acaactatcc tactccccaa aactaaatat ttctttgaaa tcccatagaa actggctccc
     1561 attactggtg tcactgctta cactgtcaat cctggcatca ctaagaccac tctggtccac
     1621 aaattcaact cgtggctgga tgtggagccc cgtgtggcag agaagctgct cgagcatccc
     1681 acccagacct ctcagcagtg cgccgagaac tttgtgaagg ccattgagct gaacaagaac
     1741 ggtgccatct ggaagttgga tctgggcacc ttggagccca tcacatggac ccagcactgg
     1801 gactcgggca tctaaaccat cccagagact ctatgggaca tggcgtttta gcttttagtt
     1861 tctttttttc cactcaattg ttacgtatat acatacacat atggcaataa ggctgatttg
     1921 aaccccgttt tgaatatgat aatacaaaat tatatttgag aaatttcaac aaaatcgata
     1981 agagctatag ctctctttta attaaaaaaa actagataaa aagagcaatg gtcagtggtg
     2041 gtcagtggtc gttgtggcct ctatcgattt ccacacaaaa actttactgt ttagtaatag
     2101 aaaagaactt gagaaaggca gccaaaagaa tgtacgatct gacgggtaag catgtctgct
     2161 atgtggctga ctgcggtggc attgcactgg agaccagcaa ggttctcatg accaagaata
     2221 tagcggtgag tggtagagag gtggtgtaga gagtggagag tggttatggt tcttaaacaa
     2281 gtctctagtc tctagtctct agtctcttgg ctgctgctgg tagctgtcga aactaattaa
     2341 gttaatgact ttttcatgtt ttgtttttgt ccgttcaaag ctacaattaa gtttatgttt
     2401 tgagtttgat ctacttttgc ttaagggaag tactttcgat tacggattgc tggcatataa
     2461 aaatagatac caaggaatgt gcatcatatc atattgattc catcttgatt ctttgcccag
     2521 aaactggcca tccttcagag cgtggaaaac cccccggcca tcgctcagtt acaatcgatt
     2581 aagcacagca cacagatctt cttctggacc ttcgatgtga ccatggcccg cgaggagatg
     2641 aagaagtact tcgatgaggt catggtccag atggactaca tcgatgtgct gatcaatggg
     2701 gccaccctgt gcgatgagcg gaacattgat gccaccatca atacgaatct gaccggcatg
     2761 atgaacaccg tggccactgt gctgccctat atggaccgaa agatgggcgg ttcgggtgga
     2821 ctgatcgtga atgtcacctc cgtcatagga ctggatccat cgccggtctt ttgtgcgtac
     2881 agtgcctcaa agtttggtgt gattgggttc actcgaagtc tagcggtgag tgggcgagat
     2941 cttaacttga aaatgctctc taatgtcttc cattctttcg caggatcccc tgtattacac
     3001 ccaaaatggt gtggctgtca tggccgtctg ctgtggtccc accaaagtgt ttgtcgatcg
     3061 ggaactgact gccttcctgg aatacggcca gtcctttgcc gatcgcctgc gtcgtgcccc
     3121 ctgccaatcg acggccgtct gcggccagaa tatagtaaat gccatcgaga gatcggagaa
     3181 cggacagatc tggattgccg acaagggcgg cctggagtcg gtggcccttc actggtattg
     3241 gcatatggcc gatcagtttg tgaactatat gcagagcact gacgatgagg atcaggagtt
     3301 ttttttgggc cagcgatgag gcatgccttg atccacggaa taaaacatca aatgtgtcac
     3361 aaaaagtagg caacttgttt gtgggtctgg aaatagattt gaaatatttt gaggtcatat
     3421 gaataatgac ataaatgagg gtgcctgaaa gtatgccaag ttt
//