From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Arabidopsis thaliana phosphoribosylanthranilate transferase (PAT1)
Date: 6 Nov 92 18:05:22 GMT
Distribution: bionet
Checksum: 55899 10

LOCUS       ATHPHOTRAN   4143 bp ds-DNA             PLN       28-OCT-1992
DEFINITION  Arabidopsis thaliana phosphoribosylanthranilate transferase (PAT1)
            gene, complete cds.
ACCESSION   M96073
KEYWORDS    phosphoribosylanthranilate transferase; tryptophan biosynthesis.
SOURCE      Arabidopsis thaliana (strain Columbia) (library: lambda-EMBL3)
            rosette leaf DNA.
  ORGANISM  Arabidopsis thaliana
            Eukaryota; Plantae; Embryobionta; Magnoliophyta; Magnoliopsida;
            Dilleniidae; Capparales; Brassicaceae.
REFERENCE   1  (sites)
  AUTHORS   Elledge,S.J., Mulligan,J.T., Ramer,S.W., Spottswood,M. and
            Davis,R.W.
  TITLE     lambda-YES: a multifunctional cDNA expression vector for the
            isolation of genes by complementation of yeast and escherichia coli
            mutations
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 88, 1731-1735 (1991)
  STANDARD  full automatic
REFERENCE   2  (bases 1 to 4143)
  AUTHORS   Rose,A.B., Casselman,A.L. and Last,R.L.
  TITLE     A phosphoribosylanthranilate transferase gene is defective in blue
            fluorescent Arabidopsis thaliana tryptophan mutants
  JOURNAL   Plant Physiol. 100, 582-592 (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     mRNA            join(1126..1384,1495..1606,1959..2102,2215..2376,
                     2491..2763,2847..2937,3016..3075,3165..3283,3363..3744)
                     /gene="PAT1"
     TATA_signal     1051..1058
                     /gene="PAT1"
     TATA_signal     1081..1087
                     /gene="PAT1"
     exon            1126..1384
                     /number=1
                     /gene="PAT1"
     intron          1385..1494
                     /number=1
                     /gene="PAT1"
     exon            1495..1606
                     /number=2
                     /gene="PAT1"
     intron          1607..1958
                     /number=2
                     /gene="PAT1"
     exon            1959..2102
                     /number=3
                     /gene="PAT1"
     intron          2103..2214
                     /number=3
                     /gene="PAT1"
     exon            2215..2376
                     /number=4
                     /gene="PAT1"
     intron          2377..2490
                     /number=4
                     /gene="PAT1"
     exon            2491..2763
                     /number=5
                     /gene="PAT1"
     intron          2764..2846
                     /number=5
                     /gene="PAT1"
     exon            2847..2937
                     /number=6
                     /gene="PAT1"
     intron          2938..3015
                     /number=6
                     /gene="PAT1"
     exon            3016..3075
                     /number=7
                     /gene="PAT1"
     intron          3076..3164
                     /number=7
                     /gene="PAT1"
     exon            3165..3283
                     /number=8
                     /gene="PAT1"
     intron          3284..3362
                     /number=8
                     /gene="PAT1"
     exon            3363..3744
                     /number=9
                     /gene="PAT1"
     polyA_signal    3647..3652
                     /gene="PAT1"
     polyA_signal    3715..3720
                     /gene="PAT1"
     polyA_signal    3724..3729
                     /gene="PAT1"
     CDS             join(1167..1384,1495..1606,1959..2102,2215..2376,
                     2491..2763,2847..2937,3016..3075,3165..3283,3363..3518)
                     /EC_number="2.4.2.18"
                     /product="phosphoribosylanthranilate transferase"
                     /codon_start=1
                     /translation="MVIAVATTSSIVSGIKLSGILTSFNAVDDASSSCGRSNLTGVRI
                     FPTLSRRRFSSIGAVSPIRGDAQSSFSRSSFACSQNLGLSGGFSAAEALPPACANASP
                     SSIKSFNQLIETLIDRVDLSETEAESSLEFLLNEANEALISAFLVLLRAKGETYEEIV
                     GLARAMMKHARKVEGLVDAVDIVGTGGDGANTVNISTGSSILAAACGAKVAKQGNRSS
                     SSACGSADVLEALGVVLDLGPEGIKRCVEEGGIGFMMSPMYHPAMKIVGPVRKKLKIK
                     TVFNILGPMLNPARVSYAVVGVYHKDLVVKMAKALQRFGMKRALVVHSCGLDEMSPLG
                     GGLVYDVTPEKIEEFSFDPLDFGIPRCTLEDLRGGGPDYNADVLRRVLSGESGAIADS
                     LILNAAAALLVSNRVQTLAEGVTVAREVQSSGKAIKTLDSWINISNLAQKSQ"
BASE COUNT     1155 a    745 c    910 g   1333 t
ORIGIN      
        1 gtcgacgtgg tgcggtgggt tgatgacacc cgtttctgga agaatccgac atcaacttta
       61 ctagttcacg cacttgtcgt gatgctgatt tggttcccgg atcttatcgt accgacatta
      121 gcgttttact tgttcgtgat tggtgcgtgg aattataggt tcaggtcacg tgctgctcta
      181 ccacatttcg atccaagact ctcgttagct gatgcagctg atagagacga gctcgacgag
      241 gagtttgacg tcgtaccgag caaccgacca ccggagatgg ttcggttgag gtacgataag
      301 ctacgaaacg tcggagctag agttcaaacg attcttggtg aagtggctgc gcaaggggag
      361 aagatgcaag ctttggtgac gtggcgtgac ccacgagcga ctggtatatt cgtggggctg
      421 tgtttctttg tggcgttggt gttgtatctt gtgccgacga agatggtggc tatggcgtca
      481 gggttttatt acttccggca tcctattttc cgtgatcgga aaccttctcc ggtgttaaat
      541 ttcttccggc gactaccatc attatctgat cggctcatgt aatattgttt ttttttttgg
      601 aggggatgta tatatagttt gggtcgttta accttgttgg gccttattgg gttataatgg
      661 gccactaaat ggatttttat atgatttcct attataggtg ctgtgtttga ccatgtctaa
      721 aacaaagaag tgtttggtat gacaaaattg atgaacctac tttttgagca gagtggtaaa
      781 tgtgcggaaa taagcacaca aggaaatcgt tgttggtgac taacaaaagg agttatccac
      841 tgcccaagca ttaccaacta ccaccaacca aactcaattt ggaaatttca aaggtccatt
      901 agtcaaagca gcattaagtc attcagtaat caacaaatat attcatatga aacccaaaat
      961 aaaataaaat aaacactttt caagaaaaaa ataaaatact gagtactaaa ctggaaaccc
     1021 tttataagca aaaagaccaa aagttaaaag ttaattaaga tttggtttaa ggcgctttgc
     1081 tttattagag aggaaggaac gaaccatcat agaaatctct ccttcgttca agtttttctc
     1141 tactgtgttg gttgagcaat cgagtgatgg ttattgcggt ggcgacgacg agctccattg
     1201 tttccggaat caagctttca gggatcttaa cttcgttcaa cgccgtagat gatgcgtcca
     1261 gtagttgcgg aagaagcaac ttgaccggag ttagaatttt tccgacattg agtcggcgac
     1321 gattctcatc gatcggagct gtttccccaa ttcgtgggga tgctcaatct tcctttagtc
     1381 gcaggtaaag cctcgatttt tgggtttagg tgtctgctta ttagagtaaa aacacatcct
     1441 ttgaaattgt ttgtggtcat ttgattgtgc tcttgatcca ttgaattgct gcagctcttt
     1501 cgcttgctct cagaatctcg gtttgagcgg tggatttagt gcagctgaag ctctgccacc
     1561 tgcttgtgca aatgctagcc cttcttccat taaatctttc aaccaggtga ctcttcattt
     1621 ctattttcag gataagtttg tggtttgatg atcccttgtt tctaagtctc ttgatttgtg
     1681 aacaattcag ggtttttata tatctgtatc tgtatttctg agttgggttg agttattggc
     1741 ttcatgctga ctaaacatgt ttggccctgt gtgttcttcg aatttcattt taattgcttg
     1801 atgggtttta ttgacttgag taaaagtgta ctactttata ggttttaaaa gagttttact
     1861 tttggtgatt ttccaatggc tttgaagaga gagatttaaa tcatggggtt actatgttct
     1921 catactgcgt ttgctctgtt ctgttgtttt tctttcagtt gattgaaacc ttgattgatc
     1981 gggttgatct atctgaaact gaggctgaat catctcttga gtttttgctg aatgaggcaa
     2041 acgaggcgct gatcagtgcc tttctagttc ttctgagagc taaaggagag acatacgaag
     2101 aggtaagtat ccattaagtc aatctaggct tataataaaa aagtatacat aatcctatat
     2161 tactgcactt caataagtta actcaacgtt cactttaatt cggatattgg gtagattgtg
     2221 gggttagcta gggcaatgat gaagcatgcc aggaaagtgg aaggattagt tgatgctgtg
     2281 gacatagttg gaacaggtgg tgatggagca aacacagtca atatatcaac tggatcttca
     2341 atacttgctg cagcttgtgg tgcaaaagta gcaaaggtat aacgaatgag aaaatcctta
     2401 aaagaaacga tatatttaaa ttttgactat ctgcattaat gctaaatgat cttcgtctgg
     2461 tcgtctaact aatggatact tattttgcag caagggaatc gttcaagttc ttctgcttgt
     2521 ggaagtgctg atgtactaga ggcactagga gtggttctgg acttgggacc agagggcatt
     2581 aaaagatgtg ttgaagaagg ggggatcggt tttatgatgt caccaatgta tcatccagct
     2641 atgaagatcg ttggtcctgt tcggaaaaag cttaaaatta aaactgtttt taacatattg
     2701 ggacctatgc ttaatcctgc tagagtttct tatgctgtcg ttggtgtata ccacaaagat
     2761 ctggtacatt tacttattgc tgaaattagt ttgttcatca acaaaaacaa gacatttact
     2821 taccatttgc ttgtgtggtt tcataggttg ttaagatggc aaaggcactg caacgatttg
     2881 gaatgaaaag agcactggtg gttcattcat gtggtctaga cgaaatgagt ccgttaggta
     2941 agattatctc ttcccaaaat tgattacttt tattattgaa caattattaa ccaatcatgg
     3001 cttaacgaaa aacaggagga ggactagttt atgatgtaac tccggaaaag atcgaagaat
     3061 tctcattcga cccatgtaat gtttcctcta gctttttatc ccttctctaa aatgaatatt
     3121 gcagatgcag cttaatgaaa ttgaagctta tttctttttg acagtggact ttggtattcc
     3181 tcgttgtact cttgaggatt tgcgaggtgg aggtccagac tacaatgcgg atgtgctaag
     3241 acgtgtgctt tcaggggaaa gtggagcaat tgcggattca ttggtatgtt ttgatttgcc
     3301 gattcaacat tctctgaact tctgcaattt gttcatcctt ttttttcaaa atcttgtttc
     3361 agatcctaaa cgcagctgca gctcttctgg ttagcaaccg agttcaaacg ctagctgaag
     3421 gagtaaccgt ggcacgtgaa gtacaatcgt ctgggaaagc tatcaagacg cttgattctt
     3481 ggataaacat ctcaaactta gctcagaaat ctcagtgacc agaagaggct tatgatattg
     3541 ttgtattcaa cggatcacct ttgcaagcaa ttgtagatga agatggaggc aatgtgccaa
     3601 aggaagaagc tggagttatt tgtaacttaa gttttctttg tcaaacaata aagagaagag
     3661 accggagcga gctctataca cattcatcat gtaaacacta caacaataat ctttaataaa
     3721 atgaataaat ctgttttact caaacggagt tcagattcat cattgggata caaatcaaac
     3781 ctaagtagta agtagaaaaa caaaattata aaattagtcc taacattgaa tcacagacca
     3841 taatcttgtt ccgaagttct tcactaattc tgcaactaca cctaaaatat catcatcgtc
     3901 atggtgtcta ttctttggag actttcacac gcatctctgt cggtttgatg agcttgaacg
     3961 tacaaacatc tccgaccagt aaacctaact ccttggccaa acgactccat ccaccagaga
     4021 atcttgattg tacatcagtg accacatatg taacatccca tgacttcttt cctctcggat
     4081 aatgaatctt gaacatcgtt gtctcagttg gcatatgcat atccacaaat ttctttggga
     4141 tcc
//