From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: S.curacoi polyketide synthase gene cluster
Date: 15 Sep 92 10:06:11 GMT
Distribution: bionet
Checksum: 10092    12

LOCUS       STMPKSGC     5469 bp ds-DNA             BCT       15-SEP-1992
DEFINITION  S.curacoi polyketide synthase gene cluster
ACCESSION   X62518
KEYWORDS    CurB gene; CurC gene; CurD gene; CurE gene; CurF gene; CurG gene;
            acyl carrier protein; beta-ketoacyl synthase; curA gene; cyclase;
            gene cluster; polyketide synthase.
SOURCE      Streptomyces curacoi DNA.
  ORGANISM  Streptomyces curacoi
            Prokaryotae; Firmicutes; Streptomycetaceae.
REFERENCE   1  (bases 1 to 5469)
  AUTHORS   Bergh,S. and Uhlen,M.
  TITLE     Complete sequence of a polyketide synthesis gene cluster of
            Streptomyces curacoi
  JOURNAL   Unpublished (1991)
  STANDARD  full automatic
REFERENCE   2  (bases 1 to 5469)
  AUTHORS   Bergh,S.
  JOURNAL   Unpublished (1991)
  STANDARD  full automatic
REFERENCE   3  (bases 1273 to 3464)
  AUTHORS   Bergh,S. and Uhlen,M.
  TITLE     Analysis of a polyketide synthesis-encoding gene cluster of
            Streptomyces curacoi
  JOURNAL   Gene 117, 131-136 (1992)
  STANDARD  full automatic
COMMENT     *source: strain=ATCC 13385; *source: is_germline=Y;
            From EMBL    entry SCPKSGC;  dated 14-OCT-1991.
FEATURES             Location/Qualifiers
     CDS             201..1304
                     /gene="curD"
                     /codon_start=1
                     /translation="MTTSPYTSQSAFDGSRMRVTLLVDPSRRRQQEFLDAYEQMRDRV
                     ASVPDLGDQLCQSLENPSQWLITSEWAAPPYLAWVNSEDHLETVKPLQSCVRDLRSMR
                     YGIVRETGGTGPHLGGLQTAVRVGDGVTRHALTFTVKPGSESKVAEVLAGYAPPQADV
                     DDSTRLRRTTLFMHGNRVVRTVEVEGDLMAALRHVARQPEVRAVEEAMNPHLEQHRDL
                     TDPESARAFFARAAMPAVHHVARGGPEPAGLRRHALYYPARPGCGMALARLLSRQDET
                     AAADPAGPVTAATVFQREEIVVRLIDVPGDPETDPVMALGIHGKRKAAVLARLLDGAA
                     LGLAGPPTSEREATRLLTHADMTLITDRRAAQP"
     CDS             1349..1813
                     /gene="curC"
                     /codon_start=1
                     /translation="MDKMRPRVVDVKEIEPNRKRGGDLRTLLTPVTVGATSGFMGLAI
                     MRPGERISEHYHPYSEEFVYVVEGRLEVDLDGETFPLRADQGLMIPIDMRHRFRNVGD
                     EEARMVFHLSPLAPKPSLGHVDTEAPAISDDVKAYPLVQEESGRPERPGVLS"
     CDS             1810..3078
                     /product="beta-ketoacyl synthase"
                     /gene="curA"
                     /codon_start=1
                     /translation="MTRRVAVTGIGVVAPGGIGVPAFWDLLSSGRTATRGITLFDPEG
                     LRSRIAAECDFDPLAHGLDPELVERADRYIQFALVAADEAVTDSGIDFGTENPWRVAV
                     SLGSAVGGTTRLEHDYVLVSERGQRWDVDHRAAEPELHRAFSPSTLAADVAERFGAQG
                     PVQTVSTGCTSGLDAVGYAFHTIEEGRADVCIAGASDSPISPITMACFDAIKATSPNN
                     DDPEHASRPFDAHRDGFVMGEGAAVLVLEELEHARARGAHVYCEIGGYATFGNAYHMT
                     GLTSEGLEMARAIDVALDHARVDPTDIDYVNAHGSGTRQNDRHETAAVKKSLGAHAYD
                     TPMSSIKSMVGHSLGAIGAIEVVACVLALARQVVPPTANYETPDPECDLDYVPRTARP
                     RRLDHVLSVGSGFGGFQSAVLLTGPAGRKR"
     CDS             3075..4334
                     /gene="curB"
                     /codon_start=1
                     /translation="MNGSGSGIRTRRTAVTGIGVVAPNGLHADTYWKSVKEGASVLDR
                     ITREGCEHLPLRVAGEVRGFDPSALIEETFLVQTDLFTHFALAAADAALQDAGLTKAA
                     AVADSPYSVGVVTAAGSGGGEFGQRELQKLWGQGSRFVGPYQSIAWFYAASTGQISIR
                     SGFKGPCGVVASDEAGGLDAVAHAARVVERGTDVVVVGAAEAPLAPYSMVCQLGYPEL
                     STDEDPAIAYRPFTSAARGFVPAEGGAVLIVEEADRAHRRGAVVRATVAGHAATFTGA
                     SHWDQSREGLAHAIHGALDEAGCAPEEIDVVFADAMGVPEADRAEALALVDALGRHAP
                     RVPVTAPKTGIGRSYCGAALLDIAAGAGMEHGQIPPTPNVFDICHQIDLVTATARPAS
                     SAPLVLSRGLMGSNAALVLRPGPGSTA"
     CDS             4418..4678
                     /product="acyl carrier protein"
                     /gene="curE"
                     /codon_start=1
                     /translation="MTTEVTQVTVEELATLMKKAAGVTVDPRDLERRANGFADFGLDS
                     LGLLGIVGELENRYARALHRRGTCKSPRAFLDVVNGALASGA"
     CDS             4680..5138
                     /product="cyclase"
                     /gene="curF"
                     /codon_start=1
                     /translation="MAGHTENEITIAAPVDLVWDMTNDLERWPELFSEYASCEVLSRE
                     ANTVTFRLTMHPDENGKVWSWVSERTADREKLVVRARRVETGPFEYMNIVWEYEETPD
                     GTRMHWTQDFAMKPDAPVDDAGMTDIINRNSPIQMALIRDRIEEVSCTTP"
     CDS             5123..5446
                     /gene="curG"
                     /codon_start=1
                     /translation="MHHALIVARIARSPAQDIAGVFAASDPGELRHLVGVTQRSLFQF
                     GDVYMHFIEAGAGPGPRIAKVTGHPEFVDISRKLEAYVSPYDPQTWRSPRDAMGRCFY
                     HWERD"
BASE COUNT      876 a   2026 c   1834 g    733 t
ORIGIN
        1 gctgtcggcc gggcggtccg cctgctccgt tcgtccggct cgacatgcga acgaccatcg
       61 cgagccgggc agtccctcaa ttggccgcat ggctcgcggg ttcctgccgc tcaaccacga
      121 tgaagctcag ggccgaccgg agttcgctct ggtggacacg gacggccgcc gcttcgtccc
      181 gcgaaggaag ggtctgccgg atgaccactt cgccctacac gtcccagtcg gcattcgacg
      241 gctccagaat gcgggtcacc ctgctcgtcg acccctcgcg aaggcgccag caagagttcc
      301 tcgacgcgta cgagcagatg cgtgaccggg tcgcctcggt ccctgacctc ggtgaccagc
      361 tgtgccagtc cctggaaaac ccgtcccagt ggctcatcac gagtgaatgg gctgccccgc
      421 cgtacctcgc ctgggtgaac agtgaggacc atctcgagac ggtcaagccc ctgcagagct
      481 gcgtccgtga cctccgttcg atgcggtacg gcatcgtccg ggagaccggc ggcaccggcc
      541 cccacctcgg cggactccag acggccgtac gggtgggcga cggagtgacg cgccatgccc
      601 tgaccttcac cgtcaagccc ggctccgagt ccaaggtcgc ggaggtcctc gccggatacg
      661 ccccgccgca ggccgacgtc gacgactcca cgcggctgcg ccgcaccacc ctcttcatgc
      721 acggcaaccg ggtcgtgcgc accgtcgagg tggagggcga cctgatggcg gcgctgcgtc
      781 atgtcgcgcg tcagcccgag gtgcgggcgg tcgaggaggc catgaacccc catctggagc
      841 agcacaggga tctgaccgac cccgagtcgg cgcgggcctt cttcgcccgc gcggccatgc
      901 cggccgtcca ccatgtggca cgcggcggac cggagccggc cggcctgcgg cggcacgccc
      961 tgtactaccc ggcacgtccc ggttgtggga tggcgctggc ccggctgctc tcccggcagg
     1021 acgagaccgc ggcggccgac ccggccggcc ccgtgaccgc agccaccgtc ttccagcggg
     1081 aggaaatcgt cgtccgcctt atcgacgtac cgggtgaccc cgagaccgat ccggtcatgg
     1141 cgctcggcat ccacggcaag aggaaggccg ccgtgctggc ccgtctgctc gacggtgccg
     1201 cactcggcct ggcgggaccg cccacgagcg aacgtgaggc gacccgtctc ctgacgcacg
     1261 ccgacatgac gctgatcacc gaccgcaggg ccgcgcagcc ctgacagcgc agggaccccc
     1321 accccaccga ccgacctgga gacagaccat ggacaagatg cgcccgcgcg tcgtggacgt
     1381 caaagagatc gagcccaacc gcaagcgcgg cggtgacctg cgcacgctgc tcacccctgt
     1441 cacggtgggt gccaccagcg ggttcatggg cctggccatc atgcggccgg gcgaacgcat
     1501 cagcgagcac taccacccgt actccgagga gttcgtgtac gtcgtcgagg gcaggctgga
     1561 ggtcgacctc gatggcgaaa cgtttcctct ccgtgccgac cagggcctca tgatccccat
     1621 cgacatgcgg caccgcttcc gcaacgtcgg tgacgaggaa gcccggatgg tcttccatct
     1681 gagcccgctg gcaccgaagc cgagcctcgg ccacgtcgac acggaggccc cggcgatcag
     1741 cgacgacgtc aaggcgtacc cgctcgtcca ggaggagagc gggcggccgg aacgacccgg
     1801 ggtcctgtcg tgacccggcg ggtggcggtc accggcatag gcgtggtcgc tccgggaggc
     1861 atcggggtgc ccgcgttctg ggacctcctc tcgagcggcc gtaccgcgac acgtggcatc
     1921 accctgttcg accccgaggg gctgcggtcc cgcatcgccg ccgagtgcga cttcgacccg
     1981 ctcgcccacg gcctcgaccc cgagctggtc gaacgcgccg accggtacat acagttcgcc
     2041 ctggtcgccg ccgacgaggc cgtgaccgac agcggcatcg acttcggcac cgagaacccc
     2101 tggcgcgtgg ccgtgtccct gggcagcgcg gtgggcggga cgaccaggct ggagcacgac
     2161 tacgtcctcg tcagcgaacg gggacagcgc tgggacgtcg accaccgcgc cgccgagccg
     2221 gaactgcacc gcgccttctc gcccagcacg ctggccgcgg acgtcgcgga gcggttcggc
     2281 gcccagggac cggtgcagac cgtgtccacc ggctgcacct ccgggctcga cgcggtgggt
     2341 tacgccttcc acaccatcga ggagggccgc gccgacgtct gcatcgccgg ggcgtcggac
     2401 tcgccgatct ccccaatcac catggcctgc ttcgacgcca tcaaggccac gtcgcccaac
     2461 aacgacgacc cggagcacgc ctcccgcccc ttcgacgccc accgcgacgg cttcgtcatg
     2521 ggcgagggcg ccgccgtcct cgtcctggag gaactggaac acgcccgtgc gcgcggcgca
     2581 catgtctact gcgagatcgg cggctacgcc accttcggca acgcctacca catgaccgga
     2641 ctgaccagtg agggcctgga gatggcccgg gccatcgacg tcgcgctcga ccacgcgcgc
     2701 gtcgacccga ccgacatcga ctacgtcaac gcccacggct cgggcacccg gcagaacgac
     2761 cgccacgaga ccgccgcggt caagaagtcc ctgggcgccc acgcctacga cacccccatg
     2821 agctcgatca agtccatggt gggccactcg ctcggcgcga tcggcgcgat cgaggtcgtc
     2881 gcctgcgtac tcgccctggc ccggcaggtg gtcccgccga cggcgaacta cgagaccccg
     2941 gaccccgagt gcgacctgga ctacgtaccg cgcaccgcac ggccccgccg gctcgaccac
     3001 gtgctctccg tgggcagcgg cttcggcggg ttccagtccg cggtgctcct gacggggccg
     3061 gctgggagga aacgatgaac ggcagtggta gtggcatacg cacccggcgg accgccgtca
     3121 cggggatcgg tgtggtcgcg cccaacggcc tgcacgccga cacctactgg aagtccgtca
     3181 aggagggcgc gagcgtactg gaccgcatca cccgggaggg ctgcgagcac cttccgctcc
     3241 gcgtcgccgg tgaggtccgc ggattcgacc cgtcggcgct catcgaggag accttcctcg
     3301 tccagaccga cctgttcacc cacttcgccc tggccgccgc ggacgccgcc ctccaggacg
     3361 cggggctgac caaggccgcc gccgtggccg actcgccgta ctcggtcggt gtcgtcaccg
     3421 ccgccgggtc cggtggcggc gagttcgggc agcgggaact gcagaagctg tggggacagg
     3481 ggtcccggtt cgtcgggccc taccagtcca tcgcctggtt ctacgcggcc agcaccggcc
     3541 agatctccat ccgcagcggc ttcaagggcc cctgcggggt ggtggccagc gacgaggcgg
     3601 gcggcctgga cgcagtcgcg cacgccgccc gggtcgtgga gcggggcacc gacgtggtcg
     3661 tggtcggggc ggccgaggca ccgctcgccc cgtactcgat ggtctgccag ctcggctacc
     3721 ccgaactcag caccgacgag gacccggcga tagcctacag gcccttcacc tcggcggccc
     3781 gcggcttcgt gcccgccgag ggcggtgccg tactgatcgt cgaggaggcg gaccgggcac
     3841 accgccgtgg tgcggtcgtc cgcgccacgg tggcgggaca cgccgccacc ttcaccggcg
     3901 cctcgcactg ggaccagtca cgggaaggac tcgcccacgc catacacggc gccctcgacg
     3961 aggccggctg tgcgccggag gagatcgacg tggtcttcgc cgacgcgatg ggcgtacccg
     4021 aggcggaccg cgccgaggca ctcgccctcg tcgacgccct cggccggcac gcaccccgcg
     4081 tgcccgtcac ggcccccaag accggcatcg ggcggtccta ctgcggggcg gccctgctgg
     4141 acatcgcggc cggtgctggc atggagcacg ggcagatacc gccgacgccg aacgtcttcg
     4201 acatctgcca ccagatcgac ctggtgaccg ccacggcccg cccggcgagc tccgcaccgc
     4261 tggtcctgag ccggggactc atggggtcca acgcggcgct cgtgctgcgg ccaggccccg
     4321 ggtcgaccgc ctagaacggc tgccgcggcc gatgccgtgg acaggcggcg gcccggcggc
     4381 accacctccg cacctggtac gacgaaggag aacccgcatg accaccgaag tgacccaagt
     4441 gaccgtcgag gaactggcca cgctgatgaa gaaggccgcc ggtgtcaccg tcgacccgcg
     4501 cgacctcgaa cgccgggcga acggcttcgc cgacttcggg ctggactcgc tcggcctcct
     4561 cggcatcgtg ggcgagctgg agaaccggta cgcccgggcg ctgcaccgac gcgggacgtg
     4621 caagagccct cgcgccttcc tcgacgtcgt caacggcgca ctggcgagcg gagcctgagg
     4681 tggctggaca caccgagaac gagatcacca tcgccgcacc cgtcgacctc gtctgggaca
     4741 tgaccaacga cctggagcgg tggcccgagc tgttcagcga gtacgcctcc tgcgaggtgc
     4801 tctcccgcga ggcgaacacg gtgaccttcc gtctgaccat gcaccccgac gagaacggca
     4861 aggtgtggag ctgggtctcg gaacggaccg ccgaccgcga gaagctcgtc gtccgtgccc
     4921 gccgggtcga gaccggcccg ttcgagtaca tgaacatcgt gtgggagtac gaggagaccc
     4981 ccgacggcac ccgcatgcac tggacgcagg acttcgccat gaagcccgac gccccggtcg
     5041 acgacgccgg gatgacggac atcatcaacc gcaactcgcc catccagatg gccctcatcc
     5101 gggaccgcat cgaggaggtc tcgtgcacca cgccctgatc gtcgccagga tcgcccgcag
     5161 tccggcccag gacatagccg gtgtcttcgc ggcctccgac cccggcgaac tccgccacct
     5221 cgtcggggtg acccagcgct ccctcttcca gttcggcgat gtgtacatgc acttcatcga
     5281 agccggagca ggacccgggc cccgcatcgc gaaggtgacc gggcaccccg agttcgtcga
     5341 catcagcaga aagctcgaag cgtacgtcag cccgtacgac ccgcagacct ggcgcagtcc
     5401 cagggacgcg atgggccggt gcttctacca ctgggagaga gactgaacag ggagagcgac
     5461 ttaagacga
//