From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human perforin mRNA, complete cds.
Date: 29 Sep 92 10:07:04 GMT
Distribution: bionet
Checksum: 26428     5

LOCUS       HUMPER       1668 bp ss-mRNA            PRI       29-SEP-1992
DEFINITION  Human perforin mRNA, complete cds.
ACCESSION   M28393
KEYWORDS    perforin.
SOURCE      Homo sapiens cDNA to mRNA.
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 1668)
  AUTHORS   Shinkai,Y., Yoshida,M.C., Maeda,K., Kobata,T., Maruyama,K.,
            Yodoi,J., Yagita,H. and Okumura,K.
  TITLE     Molecular cloning and chromosomal assignment of a human perforin
            (PFP) gene
  JOURNAL   Immunogenetics 30, 452-457 (1989)
  STANDARD  full staff_review
COMMENT     Draft entry and computer-readable copy of sequence [1] kindly
            provided by Y.Shinkai, 11-SEPT-1989.
FEATURES             Location/Qualifiers
     CDS             1..1668
                     /product="perforin"
                     /gene="PRF1"
                     /codon_start=1
                     /translation="MAARLLLLGILLLLLPLPVPAPCHTAARSECKRSHKFVPGAWLA
                     GEGVDVTSLRRSGSFPVDTQRFLRPDGTCTLCENALQEGTLQRLPLALTNWRAQGSGC
                     QRHVTRAKVSSTEAVARDAARSIRNDWKVGLDVTPKPTSNVHVSVAGSHSQAANFAAQ
                     KTHQDQYSFSTDTVECRFYSFHVVHTPPLHPDFKRALGDLPHHFNASTQPAYLRLISN
                     YGTHFIRAVELGGRISALTALRTCELALEGLTDNEVEDCLTVEAQVNIGIHGSISAEA
                     KACEEKKKKHKMTASFHQTYRERHSEVVGGHHTSINDLLFGIQAGPEQYSAWVNSLPG
                     SPGLVDYTLEPLHVLLDSQDPRREALRRALSQYLTDRARWRDCSRPCPPGRQKSPRDP
                     CQCVCHGSAVTTQDCCPRQRGLAQLEVTFIQAWGLWGDWFTATDAYVKLFFGGQELRT
                     STVWDNNNPIWSVRLDFGDVLLATGGPLRLQVWDQDSGRDDDLLGTCDQAPKSGSHEV
                     RCNLNHGHLKFRYHARCLPHLGGGTCLDYVPQMLLGEPPGNRSGAVW"
     sig_peptide     1..63
                     /gene="PRF1"
                     /codon_start=1
     mat_peptide     64..1665
                     /product="perforin"
                     /gene="PRF1"
                     /codon_start=1
BASE COUNT      314 a    557 c    509 g    288 t
ORIGIN      Chromosome 17q11-21
        1 atggcagccc gtctgctcct cctgggcatc cttctcctgc tgctgcccct gcccgtccct
       61 gccccgtgcc acacagccgc acgctcagag tgcaagcgca gccacaagtt cgtgcctggt
      121 gcatggctgg ccggggaggg tgtggacgtg accagcctcc gccgctcggg ctccttccca
      181 gtggacacac aaaggttcct gcggcccgac ggcacctgca ccctctgtga aaatgcccta
      241 caggagggca ccctccagcg cctgcctctg gcgctcacca actggcgggc ccagggctct
      301 ggctgccagc gccatgtaac cagggccaaa gtcagctcca ctgaagctgt ggcccgggat
      361 gcggctcgta gcatccgcaa cgactggaag gtcgggctgg acgtgactcc taagcccacc
      421 agcaatgtgc atgtgtctgt ggccggctca cactcacagg cagccaactt tgcagcccag
      481 aagacccacc aggaccagta cagcttcagc actgacacgg tggagtgccg cttctacagt
      541 ttccatgtgg tacacactcc cccgctgcac cctgacttca agagggccct cggggacctg
      601 ccccaccact tcaacgcctc cacccagccc gcctacctca ggcttatctc caactacggc
      661 acccacttca tccgggctgt ggagctgggt ggccgcatat cggccctcac tgccctgcgc
      721 acctgcgagc tggccctgga agggctcacg gacaacgagg tggaggactg cctgactgtc
      781 gaggcccagg tcaacatagg catccacggc agcatctctg ccgaagccaa ggcctgtgag
      841 gagaagaaga agaagcacaa gatgacggcc tccttccacc aaacctaccg ggagcgccat
      901 tcggaagtgg ttggcggcca tcacacctcc attaacgacc tgctgttcgg gatccaggcc
      961 gggcccgagc agtactcagc ctgggtaaac tcgctgcccg gcagccctgg cctggtggac
     1021 tacaccctgg aacccctgca cgtgctgctg gacagccagg acccgcggcg ggaggcactg
     1081 aggagggccc tgagtcagta cctgacggac agggctcgct ggagggactg cagccggccg
     1141 tgcccaccag ggcggcagaa gagcccccga gacccatgcc agtgtgtgtg ccatggctca
     1201 gcggtcacca cccaggactg ctgccctcgg cagaggggcc tggcccagct ggaggtgacc
     1261 ttcatccaag catggggcct gtggggggac tggttcactg ccacggatgc ctatgtgaag
     1321 ctcttctttg gtggccagga gctgaggacg agcaccgtgt gggacaataa caaccccatc
     1381 tggtcagtgc ggctggattt tggggatgtg ctcctggcca caggggggcc cctgaggttg
     1441 caggtctggg atcaggactc tggcagggac gatgacctcc ttggcacctg tgatcaggct
     1501 cccaagtctg gttcccatga ggtgagatgc aacctgaatc atggccacct aaaattccgc
     1561 tatcatgcca ggtgcttgcc ccacctggga ggaggcacct gcctggacta tgtcccccaa
     1621 atgcttctgg gggagcctcc aggaaaccgg agtggggccg tgtggtga
//