From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: gadA=glutamate decarboxylase [Escherichia coli, Genomic, 1437 nt]
Date: 6 Jan 93 07:09:31 GMT
Distribution: bionet
Checksum: 31434 4

LOCUS       S112979      1437 bp    DNA             BCT       04-DEC-1992
DEFINITION  gadA=glutamate decarboxylase [Escherichia coli, Genomic, 1437 nt]
ACCESSION   S44575
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Unclassified.
REFERENCE   1  (bases 1 to 1437)
  AUTHORS   Smith,D.K., Kassam,T., Singh,B. and Elliott,J.F.
  TITLE     Escherichia coli has two homologous glutamate decarboxylase genes
            that map to distinct loci.
  JOURNAL   J. Bacteriol. 174, 5820-5826 (1992)
  STANDARD  full automatic
COMMENT     This entry [NCBI gibbsq 112979] was created by the journal scanning
            component of NCBI/GenBank at the National Library of Medicine.
            This sequence comes from Fig. 3.  Map location: 4046 kb.
FEATURES             Location/Qualifiers
     CDS             19..1419
                     /note="glutamaic acid decarboxylase;  GAD alpha;  For the
                     protein sequence (NCBI gibbsq 112980):  This sequence
                     comes from Fig. 3."
                     /gene="gadA"
                     /product="glutamate decarboxylase"
                     /codon_start=1
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYE
                     FPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHK
                     FGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRL
                     GREGYTKVQNASYQVAAYLADEIAKLGPYEFICTGRPDEGIPAVCFKLKDGEDPGYTL
                     YDLSERLRLRGWQVPAFTLGGEATDIVVMRIMCRRGFEMDFAELLLEDYKASLKYLSD
                     HPKLQGIAQQNSFKHT"
BASE COUNT      341 a    380 c    385 g    331 t
ORIGIN      
        1 aaatttaagg agttcgaaat ggaccagaag ctgttaacgg atttccgctc agaactactc
       61 gattcacgtt ttggcgcaaa ggccatttct actatcgcgg agtcaaaacg atttccgctg
      121 cacgaaatgc gcgatgatgt cgcatttcag attatcaatg atgaattata tcttgatggc
      181 aacgctcgtc agaacctggc cactttctgc cagacctggg acgacgaaaa cgtccataaa
      241 ttgatggatt tgtcgatcaa taaaaactgg atcgacaaag aagaatatcc gcaatccgca
      301 gccatcgacc tgcgttgcgt aaatatggtt gccgatctgt ggcatgcgcc tgcgccgaaa
      361 aatggtcagg ccgttggcac caacaccatt ggttcttccg aggcctgtat gctcggcggg
      421 atggcgatga aatggcgttg gcgcaagcgt atggaagctg caggcaaacc aacggataaa
      481 ccaaacctgg tgtgcggtcc ggtacaaatc tgctggcata aattcgcccg ctactgggat
      541 gtggagctgc gtgagatccc tatgcgcccc ggtcagttgt ttatggaccc gaaacgcatg
      601 attgaagcct gtgacgaaaa caccatcggc gtggtgccga ctttcggcgt gacctacacc
      661 ggtaactatg agttcccaca accgctgcac gatgcgctgg ataaattcca ggccgacacc
      721 ggtatcgaca tcgacatgca catcgacgct gccagcggtg gcttcctggc accgttcgtc
      781 gccccggata tcgtctggga cttccgcctg ccgcgtgtga aatcgatcag tgcttcaggc
      841 cataaattcg gtctggctcc gctgggctgc ggctgggtta tctggcgtga cgaagaagcg
      901 ctgccgcagg aactggtgtt caacgttgac tacctgggtg gtcaaattgg tacttttgcc
      961 atcaacttct cccgcccggc gggtcaggta attgcacagt actatgaatt cctgcgcctc
     1021 ggtcgtgaag gctataccaa agtacagaac gcctcttacc aggttgccgc ttatctggcg
     1081 gatgaaatcg ccaaactggg gccgtatgag ttcatctgta cgggtcgccc ggacgaaggc
     1141 atcccggcgg tttgcttcaa actgaaagat ggtgaagatc cgggatacac cctgtacgac
     1201 ctctctgaac gtctgcgtct gcgcggctgg caggttccgg ccttcactct cggcggtgaa
     1261 gccaccgaca tcgtggtgat gcgcattatg tgtcgtcgcg gcttcgaaat ggactttgct
     1321 gaactgttgc tggaagacta caaagcctcc ctgaaatatc tcagcgatca cccgaaactg
     1381 cagggtattg cccagcagaa cagctttaaa cacacctgat aacataacgt tgtaaaa
//