From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human Wilms tumor gene encoding WT1 protein, exon 6.
Date: 6 Jan 93 06:49:35 GMT
Distribution: bionet
Checksum: 43249 9

LOCUS       HUMWT1       4922 bp ds-DNA             PRI       06-JAN-1993
DEFINITION  Human Wilms tumor gene encoding WT1 protein, exon 6.
ACCESSION   D13624
KEYWORDS    Alu repeat; Wilms'tumor; polymorphisms; suppressor oncogene.
SOURCE      Homo sapiens (library: cosmid L109) DNA.
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Mammalia;
            Theria; Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (sites)
  AUTHORS   Haber,D.A., Sohn,R.L., Buckler,A.J., Pelletier,J., Call,K.M. and
            Housman,D.E.
  TITLE     Alternative splicing and genomic structure of the Wilms tumor gene
            WT1
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 88, 9618-9622 (1991)
  STANDARD  full automatic
REFERENCE   2  (bases 1 to 4922)
  AUTHORS   Yamada,M.
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
REFERENCE   3  (sites)
  AUTHORS   Gessler,M., Konig,A. and Bruns,G.A.
  TITLE     The genomic organization and expression of the WT1 gene
  JOURNAL   Genomics 12, 807-813 (1992)
  STANDARD  full automatic
REFERENCE   4  (sites)
  AUTHORS   Tadokoro,K., Fujii,H., Ohshima,A., Kakizawa,Y., Shimizu,K.,
            Sakai,A., Sumiyoshi,K., Inoue,T., Hayashi,Y. and Yamada,M.
  TITLE     Intragenic homozygous deletion of the WT1 gene in Wilms' tumor
  JOURNAL   Oncogene 7, 1215-1221 (1992)
  STANDARD  full automatic
COMMENT     Submitted (11-NOV-1992) to DDBJ by: Masao Yamada National
            Children's Medical Research Center 3-35-31 Taishido Setagaya, Tokyo
            154 Japan Phone:  03-3414-8121 x2763 Fax:    03-3414-3208.
FEATURES             Location/Qualifiers
     misc_feature    3340..3436
                     /note="This feature applies to a gene which lacks a coding
                     region feature. "
                     /gene="WT1"
     intron          <1..3339
                     /number=5
     misc_feature    1..756
                     /note="previous report: D01248/d12496 covered"
     repeat_unit     537..817
                     /note="Alu like repeat"
     repeat_unit     1546..1826
                     /note="Alu like repeat"
     variation       2938
                     /note="TaqI/SalI polymorphism (C or T)"
     variation       2953
                     /note="ApaLI polymorphism (C or T)"
     misc_feature    3160..3552
                     /note="previous report: X61635 covered"
     misc_feature    3307..3545
                     /note="previous report: M80228/M74917 covered"
     exon            3340..3436
                     /number=6
                     /product="WT1 protein"
                     /gene="WT1"
     intron          3437..>4922
                     /number=6
BASE COUNT     1202 a   1158 c   1117 g   1445 t
ORIGIN      Chromosome 11p13.
        1 ggatcctttc tatctctatt agtctatggt tcccagaccg agttgctttt taaaaactta
       61 tttagagaga attgctacca catcagatgc cagtacgttg tacttggtgt tctgggcaca
      121 ctacattggg aagtgttgaa ttgcaggttt ccacagtgtg gaattgcatt ggtgtgtttg
      181 tcctttggag acccaagtcc ccataggcgg gggcctggct gttttttttt tcctcatgga
      241 ccttgtagga gaatccactg gcatatttgg caggtacagt cttgttgctt gttgcttctt
      301 ttgtttgtgg tttgcatcca gctgtgcaaa ttgctgttcc ctttgagtgt cacaccactt
      361 aatttcatga gacagttgtc ttcaggaaag tctgttccca tgcccccacc ctccaggaac
      421 ccagctaaca cccagagctt agctgtcaag acagatttct aatttttacc cttcgatggc
      481 agaactttaa tgttgtgtcc ctagaaatcc ttcagcccta gaaaatgtgg aaagtaggcc
      541 aggggcagtg gctcactcct gtgatcctgg cactttggga ggccaaggtg ggagaatcac
      601 ttgagcccag gagtttgaga ccatcctatg gaggacttag caagaccctg tttctacaaa
      661 gataaaaata aaaatcagct gggcatggtg cggggcacct atagtcctag ctctttggga
      721 gatggaagca ggaggaacaa ttgagccagg gaattcaagg ctgcagtgag ctatgatcgc
      781 accactacat tggagcctgg gcaatagagc aagctctaaa aaagaaaaag aaagaaaatg
      841 tgtaagtcac attgtggctc catgcagatg actgaagcaa gcaaaggcag cttctcttca
      901 cagcctttga ggcaaaatgt tctggcagca ccatggcacc attctggtac tgccctcttt
      961 ctgctgccaa atggccattg tcatcacctc tcactttccc actgtctgta ccaagtaatt
     1021 gaaaggaagg cagacacagc ctctttctcc tgacaaacac attctaaggg tcagaagggc
     1081 atatgttctt tgggtttggc atttttgccc aatttttgca ggtttgaccc tggggctact
     1141 tctttgagac attccccctg ccctagataa gtcaaaaatt acttgaagga tgtggagaca
     1201 agatatggta ggttagaggg gcaggcagtt ctttctaaaa tgcttagagc cagaagtaaa
     1261 tgtgaaaccc tacagcttct gtggcctagg gggtgtggac atttcagttg tcctggcaga
     1321 cactctttcc ttcaggcact ctctcctctg ggtgagtgag tattgggtca actccaggct
     1381 tccccacacc acaccagatg gagtgggctg atccacccac cagcttacct gcctccctgc
     1441 ccaggacccc tggaaccaac tgttaggtaa tggaggcaga gaatggaatt ctcagtgctg
     1501 acagccagtt taatcccacc tggaggcttt taaaaatgct gatgcggctg ggcctggtag
     1561 ctcacacctg taatccccgc actttgggag gcagagatgg gtggatcatt taaggccagg
     1621 agttcgagac tggcctggcc aacatggtga aaccccatct ctactaaaag tacaaaaatg
     1681 agccgggtat ggtgacgtgc gcctgtaatc ccagctactc cggaggctga ggcaggagaa
     1741 tcatttgaac ccaggggcgg tggttgcagt gagccaagat cgcaccactg cactccagcc
     1801 tgggtgataa agcgagactc catctcaaaa taaataaata aatatactga tgcatccttc
     1861 taggaccaca cccccccccg ataatttttt aaaagcagat ttatagattg cacggaccgt
     1921 atgattcacc gatttagagt gtacaatgca atggttttta gtatattcac agttgtatga
     1981 ccattaccac aattttagga cgttttcagc agtcattttc tttgccttcc caactccccc
     2041 agttcaaggc acccaaccac taatctagtt tctgtcccta cagatttgct tattctggac
     2101 attttataga aatgcaatca tactacatgt ggtcttctgt gaatggcttc ttagcacggt
     2161 attttaaagg ttcatccata ttgtagcatg tattggtact ttattccttt tcattgctga
     2221 ataatattcc attgtatgaa gatactctct ttgatttatc tattcatcag ttgatggaca
     2281 tttgggttga ttctactttt tggctcttag gaatatgctg ctaatgcaca atgtatacaa
     2341 gtttttgtgt ggacgtctgt tttcatttcc cttgggaata tccctaagag tgggatttct
     2401 gggtcatatg ttaactttat gtctaacctt ttgaggaact gccagatatt ttgcaaagca
     2461 gctgcatcat ttcacagagc tatttcataa gagctctcct cggtgcttct aggtgtaatt
     2521 ctaatgtgct gctggtgtgg gtgaaaaacc tcagtctgcc tcatctcatc tggagtaaaa
     2581 taactgtaga gcagatagag gcattgtatc tgaaggagag aataatttta gaacagaaca
     2641 ttcgttcctt tcaagttgtc cttttcagca ttgcacatct ttcagctctt cctatgtagt
     2701 cgaagcttaa cctgagcacc agttagggga ctagtcagtg gaatgctgga ggaagattct
     2761 tcagtgccca cctacagcac aggctggcta ccaagagtta agctgatctg gaaaactgac
     2821 ctgtcattta agcagagtga ggccacagat atgtaatccc cacttctgag gacatgacat
     2881 ctcttcttgc gtcatcactt ttttggtgct atctggtaaa aactgagtca tgaggtgccg
     2941 acaaattcag tgcacttaac gtgaagcttg attgaggcat tctgctcatt cctgagatac
     3001 ctgcagctgt cagccctgcc gtctagatgg taaacaactt gccgaggatg aaaccctgcc
     3061 actgtggtct tcctcggctt gtctgagcga gacctagacc gtgacggtgc acgcctacga
     3121 gaatcggagg atgagggagg ggctgcgagg tggcggccag gatccagggg cctcgctcct
     3181 cagaaccctt catctaaagt ggccccatgg aggaggcaaa aaccaactat ttttgttctg
     3241 tgagctttaa aaaccatcat tccctcctga ttgcagataa gcatttccaa atggcgactg
     3301 tgagcacact gagccttttt cccttctttg tgtttgcagc cacagcacag ggtacgagag
     3361 cgataaccac acaacgccca tcctctgcgg agcccaatac agaatacaca cgcacggtgt
     3421 cttcagaggc attcaggtga gcacacagac aggccccagc ggaagggggc cgcactgcct
     3481 cttcctactt accggccctt tagttcctta ctgatggact ctttggccct ggcagcctct
     3541 tcttcactgc aggctccttt aacatcaggg acacctgctc gtcccctggg tctgataagg
     3601 cggctgtgtc ctctcctaga gaggctgccc cgctgcctga cgctgcgcaa gatgcgtcct
     3661 aacagaatcc tctcctttct gcctttactg ctccatttga cttctccagg gcgggctgtg
     3721 cagcttttgt atttaagcta attgggcttg aattaaccgc ttggaccttt gacttctaag
     3781 atttggtcta catcgtaaag tgtccacagg tagactcaca tcttgccaag tgttgatgat
     3841 catcatacta actgtaccgc ctatggctgg acattttcta tatactaggc attgtgctaa
     3901 gctttataca tggactttag aaaaatggac tttaccctat tagtaccatt ttgtattgat
     3961 gtggtacatt tgttataatt gatgagccaa tattgataaa ttattgttaa ctaaagccca
     4021 tagtttacat tagggttcac tttttgtgtt gtacagttct atgggttttg aaaaatgcat
     4081 aatgccatgt acctattatt ctagtatcat acagaatagt ttcactgccc ttaaaattcc
     4141 ctacactcca cctgttcttc cctccctctt cttctcttcc caataatcac cggtcttttt
     4201 tttttctttt tctttttttt ttttttgaga tgggagtctc gctctgtcac ccaggctgga
     4261 gtacagtggc gctatcttgg ctcactgcaa gctccgcctc cagggttcac accattctcc
     4321 tgcctcagcc tcccgagtag ctgggactac aggtgcccac caccacgcct ggctaatttt
     4381 ttgtattttt agtagagacg gggtttcaca gtgttagcca ggatggtctt gatctcctga
     4441 cctggtgatc cactcgcctc ggcctcccaa agtgctggga ttacaggcgt gagccactgt
     4501 gcccggctcg gtcttttttc agaatgtcat atagttggaa tcatactaga tgtagcattt
     4561 tcaggctgac ttctttctct tagcaatatg catgtaaggt ttctccttgt ctatttgtgg
     4621 cctttccccc ttcatttcat ctgcaataca cacttatgag gtgggtactg ttattctctc
     4681 catttcatgg atggggaaac tgaagcccag aggtgatgaa taatgtcaga atcacatcgc
     4741 tagtaaatat tggagccgag actaataaca caggtcttgc ctgacttcag accccctgtt
     4801 tatacctact tcacgatact gcttcttagg gaggcagagg atggtgaaca gtttgtggga
     4861 gggagaagtc tccaaggagc atgaagaaag taaagtataa aggtaaaagt tttggagaat
     4921 tc
//