From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Ascaris suum phosphoenolpyruvate carboxykinase (PEPCK) gene,
Date: 15 Sep 92 10:04:58 GMT
Distribution: bionet
Checksum: 23194     6

LOCUS       NEMPEPCK     2713 bp ss-mRNA            INV       15-SEP-1992
DEFINITION  Ascaris suum phosphoenolpyruvate carboxykinase (PEPCK) gene,
            complete cds.
ACCESSION   L01787
KEYWORDS    phosphoenolpyruvate carboxykinase.
SOURCE      Ascaris suum (library: lambda ZAP11) adult pharynx; nerve cord cDNA
            to mRNA.
  ORGANISM  Ascaris suum
            Eukaryota; Animalia; Eumetazoa; Nematoda; Secernentea; Rhabditia;
            Ascaridida; Ascaridina; Ascaridoidea; Ascarididae.
REFERENCE   1  (bases 1 to 2713)
  AUTHORS   Geary,T.G., Winterrowd,C.A., Alexander-Bowman,S.J., Faureau,M.A.,
            Naly,S.C. and Klein,R.D.
  TITLE     Ascaris suum: Cloning of a cDNA encoding phosphoenolpyruvate
            carboxykinase
  JOURNAL   Unpublished (1992)
  STANDARD  full staff_entry
FEATURES             Location/Qualifiers
     CDS             160..2091
                     /function="carboxylates PEP to oxaloactetate"
                     /EC_number="4.1.1.32"
                     /note="function is reverse of PEPCK function in
                     vertebrates"
                     /product="phosphoenolpyruvate carboxykinase"
                     /gene="PEPCK"
                     /note="putative"
                     /codon_start=1
                     /translation="MRCRSLSHFKDDDFAVVSEVVTHKQNHIPVIKGDFVSLPKHVQR
                     FVAEKAELMKPSAIFICDGSQNEADELIARCVERGVLVPLKAYKNNYLCRTDPRDVAR
                     VESKTWMITPEKYDSVCHTPEGVKPMMGQWMSPDEFGKELDDRFPGCMAGRTMYVIPY
                     SMGPVGGPLSKIGIELTDSDYVVLCMRIMTRMGEPVLKALAKNNGEFVRCVHSVGQPK
                     PVATKVINHWPCNPEKTIIAHRPAEREIWSFGSGYGGNSLLGKKCFALRIAMNIGYDE
                     GWMAEHMLIMGVTSPKGEERFVAAAFPSACGKTNLAMLEPTIPGWKVRVIGDDIAWMK
                     FGADGRLYAINPEYGFFGVAPGTSHKTNPMAMASFQENTIFTNVAETADGEYFWEGLE
                     HEVKNPKVDMINWLGEPWHIGDESKAAHPNSRFTAPAGQCPIIHPDWEKPEGVPIDAI
                     IFGGRRPEGVPLVFESRSWVHGIFVGACVKSEATAAAEHTGKQVMHDPMAMRPFMGYN
                     FGRYMRHWMKLGQPPHKVPKIFHVNWFRQSADHKFLWPGYGDNIRVIDWILRRCSGDA
                     TIAEETPIGFIPKKGTINLEGLPNVNWDELMSIPKSYWLEDMVETKTFFENQVGSDLP
                     PEIAKELEAQTERIKALKE"
BASE COUNT      683 a    624 c    701 g    705 t
ORIGIN
        1 gaattccgtc acctttcttc tcacattttg tcttcactcc agcaacatct gaacccgccc
       61 atggttccgt caccgcatat gcagccatga gaggttcttc agtcattcgg ccaaggaact
      121 tcttcttgat gggttcggaa ttccgtttga ggtaacaata tgcggtgccg ctctctttct
      181 catttcaaag acgatgattt cgctgttgtc agcgaggttg tcactcataa gcagaatcac
      241 atccctgtta tcaagggtga ttttgtgagc ctcccgaagc atgtgcaacg cttcgttgcc
      301 gaaaaggctg agctgatgaa accaagcgca atcttcattt gcgacggttc ccagaatgaa
      361 gccgatgagt taattgccag atgcgtcgag cgtggtgtgt tggttcctct caaggcctac
      421 aagaacaact acctgtgccg tacggatcca cgtgatgtgg ctcgtgttga gtcaaagaca
      481 tggatgatca caccggagaa gtacgactca gtttgccata cgcctgaagg tgtcaagccg
      541 atgatgggac agtggatgtc tcctgacgag ttcggcaaag aactggacga tcgcttccct
      601 ggatgcatgg ctggaagaac gatgtatgtg attccatatt cgatgggtcc agtcggtggt
      661 ccgctatcga aaatcggcat tgagctaacc gattccgact acgtggtgtt gtgcatgcgt
      721 atcatgactc gcatgggtga acccgtactg aaggctcttg ccaagaacaa tggggaattc
      781 gtccgatgtg tgcactctgt tggtcaacca aaaccggtcg ccacgaaggt gatcaaccat
      841 tggccgtgca atccggagaa gacgattatt gctcatcgac cagctgaaag agagatttgg
      901 tcgttcggtt cgggatatgg tggcaattcg ctgcttggca aaaagtgctt cgcgctgcgt
      961 attgcaatga acattggcta tgacgagggt tggatggccg agcacatgct aatcatgggt
     1021 gtcacaagcc cgaagggtga ggagcgtttc gtagcagctg cattcccgtc tgcatgtggt
     1081 aagaccaatt tggctatgct tgagccgacc attcctggat ggaaagtccg tgtcattggt
     1141 gacgatattg cgtggatgaa atttggagca gatggacgtc tttacgcaat caatcccgag
     1201 tacggtttct ttggtgttgc gccgggaacg tcgcacaaga cgaatccgat ggccatggcc
     1261 agcttccagg agaacacgat cttcacaaac gtggcggaga cagccgatgg cgagtatttc
     1321 tgggagggtc tggagcatga agttaaaaac ccgaaagtcg acatgatcaa ctggcttggc
     1381 gagccatggc acatcggcga tgagagcaag gcagcgcacc cgaactcgcg gttcactgct
     1441 ccagccggtc aatgccctat tatccatcca gattgggaga agcccgaggg cgtaccaatt
     1501 gatgccatca tcttcggtgg ccgaaggcct gagggagtgc cgttggtgtt tgaatcacgc
     1561 agctgggtgc atggtatctt tgttggtgcc tgtgtgaaat ccgaggccac agccgctgcc
     1621 gaacatactg gcaagcaggt gatgcatgac ccgatggcta tgcgtccatt catgggatac
     1681 aactttggca ggtatatgcg ccactggatg aagctcggac agccaccaca taaggtgccc
     1741 aagatcttcc acgtgaactg gttcagacaa agtgccgatc acaagttctt gtggcccgga
     1801 tatggcgata atattcgtgt aatcgactgg atcctgcggc gttgcagtgg tgacgccaca
     1861 atcgccgaag agactccgat tggctttatc ccaaagaagg gaacgatcaa tctggaaggt
     1921 ctaccaaacg tcaattggga tgagttgatg tcgatcccca agtcctactg gctagaggat
     1981 atggtggaaa ccaaaacttt cttcgaaaac caggtggggt ctgatctccc tccagaaatc
     2041 gcgaaggagt tggaagcaca gacagaacgt atcaaggcgc tgaaggaata agctggacgc
     2101 atgccatgct gtcttcggtc gcatcaacaa cgtcctcagc ttcaatcgat ttttgagtgt
     2161 tgaaaacgaa ttgttgaatc agctcctcgc ttgtttttag acgagacccg agccttctga
     2221 ggcagtaatc tatctatatc cctatcttgt ctgacttttt tcaaattgtt tctgttctag
     2281 actccaggct cttctatctc tgtctcgact ttgctgttcg gtctttctga tcgttcagac
     2341 agcatcgtcc cattgtaccg tttgtactct gaacttcttg tggctatata tctttactca
     2401 ctcttcatcg gacaccagct attttttttt aaatgcggag tgagtagcgg caccaggcat
     2461 gaatttgcgc cggcttatag aatgcttttt acgcaaatgt gcgtttatga atatctaaat
     2521 agtgttagcg ctccgcgctg gttgcgttat aatgcaatgg tctccaaagt aattgttctc
     2581 atgtgatagg ttttaccatg tatcaacaca gaggtgtaca caactgtgtt agcgttgtgc
     2641 atcaacaata aaaaatttaa aatgaaaaaa aaaaaaggtt taaaaaaaaa aaaaaaaaaa
     2701 aaaaaaagaa ttc
//