From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: P.aeruginosa phaC1, phaC2 and phaD genes
Date: 28 Dec 92 21:31:05 GMT
Distribution: bionet
Checksum: 35992 14

LOCUS       PSEPHAC1C2   7311 bp ds-DNA             BCT       01-DEC-1992
DEFINITION  P.aeruginosa phaC1, phaC2 and phaD genes
ACCESSION   X66592
KEYWORDS    PHA-depolymerase; PHA-synthase; PHA-synthase1; PHA-synthase2.
SOURCE      Pseudomonas aeruginosa DNA.
  ORGANISM  Pseudomonas aeruginosa
            Prokaryotae; Gracilicutes; Scotobacteria; Pseudomonadaceae.
REFERENCE   1  (bases 1 to 7311)
  AUTHORS   Timm,A. and Steinbuechel,A.
  TITLE     Cloning and molecular analysis of the poly(3-hydroxyalkanoic acid)
            gene locus of pseudomonas aeruginosa PAO1
  JOURNAL   Eur. J. Biochem. 209, 15-30 (1992)
  STANDARD  full automatic
COMMENT     *source: strain=PAO1;
               From EMBL    entry PAPHAC1C2;  dated 30-SEP-1992.
FEATURES             Location/Qualifiers
     terminator      850..891
     prim_transcript 943..>7311
                     /note="transcriptional start1"
     prim_transcript 1199..>7311
                     /note="transcriptional start2"
     terminator      6618..6640
     misc_feature    complement(6651..7311)
                     /note="ORF4"
     CDS             <1..381
                     /note="ORF1"
                     /codon_start=1
                     /translation="LQGRLPIRVELKALSPNDFERILTEPHASLTEQYRELLKTEGLA
                     IEFAEDGIKRLAEIAWQVNEKTENIGARRLHTLLERLLEEVSFSAADLASEHSDKPIL
                     IDAGYVNSHLGELAEDEDLSRYIL"
     CDS             476..847
                     /note="ORF2"
                     /codon_start=1
                     /translation="MRIPSAIQLHKASKTLTLRYGEDSYDLPAEFLRVHSPSAEVQGH
                     GNPVLQYGKLNVGLVGVEPAGQYALKLSFDDGHDSGLFTWDYLYELATRKDQLWADYL
                     AELASAGKSRDPDESVVKLML"
     CDS             1266..2945
                     /gene="phaC1"
                     /product="PHA-synthase1"
                     /codon_start=1
                     /translation="MSQKNNNELPKQAAENTLNLNPVIGIRGKDLLTSARMVLLQAVR
                     QPLHSARHVAHFSLELKNVLLGQSELRPGDDDRRFSDPAWSQNPLYKRYMQTYLAWRK
                     ELHSWISHSDLSPQDISRGQFVINLLTEAMSPTNSLSNPAAVKRFFETGGKSLLDGLG
                     HLAKDLVNNGGMPSQVDMDAFEVGKNLATTEGAVVFRNDVLELIQYRPITESVHERPL
                     LVVPPQINKFYVFDLSPDKSLARFCLRNGVQTFIVSWRNPTKSQREWGLTTYIEALKE
                     AIEVVLSITGSKDLNLLGACSGGITTATLVGHYVASGEKKVNAFTQLVSVLDFELNTQ
                     VALFADEKTLEAAKRRSYQSGVLEGKDMAKVFAWMRPNDLIWNYWVNNYLLGNQPPAF
                     DILYWNNDTTRLPAALHGEFVELFKSNPLNRPGALEVSGTPIDLKQVTCDFYCVAGLN
                     DHITPWESCYKSARLLGGKCEFILSNSGHIQSILNPPGNPKARFMTNPELPAEPKAWL
                     EQAGKHADSWWLHWQQWLAERSGKTRKAPASLGNKTYPAGEAAPGTYVHER"
     CDS             3098..3955
                     /gene="phaD"
                     /product="PHA-depolymerase"
                     /codon_start=1
                     /translation="MPQPFVFRTIDLDGQTIRTAVRPGKEGSTPLLIFNGIGANLELV
                     FPFVQALDPELEVIAFDVPGVGGSSTPSVPYRFPGLAKLAARMLDYLDYGQVNAIGVS
                     WGGALAQQFAHDYPERCKKLILAATSAGAVMVPGKPKVLMRMASPRRYIQPSYGVHIA
                     PDIYGGAFRRDPKLAMAHASKVRSSGKLGYYWQLFAGLGWTSIHWLHRIRQPTLVLAG
                     DDDPIIPLINMRVLAWRIPNAELHVIDDGHLFLVTRAESVAPIIMKFLAEERRRAVMH
                     PRPFLPKTG"
     CDS             4259..5941
                     /gene="phaC2"
                     /product="PHA-synthase2"
                     /codon_start=1
                     /translation="MREKQESGSVPVPAEFMSAQSAIVGLRGKDLLTTVRSLAVHGLR
                     QPLHSARHLVAFGGQLGKVLLGDTLHQPNPQDARFQDPSWRLNPFYRRTLQAYLAWQK
                     QLLAWIDESNLDCDDRARARFLVALLSDAVAPSNSLINPLALKELFNTGGISLLNGVR
                     HLLEDLVHNGGMPSQVNKTAFEIGRNLATTQGAVVFRNEVLELIQYKPLGERQYAKPL
                     LIVPPQINKYYIFDLSPEKSFVQYALKNNLQVFVISWRNPDAQHREWGLSTYVEALDQ
                     AIEVSREITGSRSVNLAGACAGGLTVAALLGHLQVRRQLRKVSSVTYLVSLLDSQMES
                     PAMLFADEQTLESSKRRSYQHGVLDGRDMAKVFAWMRPNDLIWNYWVNNYLLGRQPPA
                     FDILYWNNDNTRLPAAFHGELLDLFKHNPLTRPGALEVSGTAVDLGKVAIDSFHVAGI
                     TDHITPWDAVYRSALLLGGQRRFILSNSGHIQSILNPPGNPKACYFENDKLSSDPRAW
                     YYDAKREEGSWWPVWLGWLQERSGELGNPDFNLGSAAHPPLEAAPGTYVHIR"
     CDS             5997..6614
                     /note="ORF3"
                     /codon_start=1
                     /translation="MKTRDRILECSLLLFNEQGEPNVSTLEIANELGISPGNLYYHFH
                     GKEPLVMALFERFQAELAPLLDPPEEVRLGAEDYWLFLHLIVERLAHYRFLFQDLSNL
                     TGRLPRLARGIRTWLGALKRTLATLLARLKADRQLRSDAPALGQLVEEITLTLLFSLD
                     YQRVLGSKGEVRTVVYQIMMLVAPHLRSEAQRSAESLAQRYLGPE"
BASE COUNT     1272 a   2580 c   2289 g   1170 t
ORIGIN      
        1 ctgcagggcc gcctgccgat ccgcgtggag ctcaaggccc tcagtccgaa cgatttcgag
       61 cgcatcctca ccgagccgca tgcctcgctc accgagcagt accgcgagct gctgaagacc
      121 gaggggctgg ccatcgagtt cgccgaggac ggcatcaagc gccttgccga gatcgcctgg
      181 caggtcaacg agaagaccga gaacatcggt gcccgccgcc tgcatacgct gctcgagcgg
      241 ctgctggaag aggtctcgtt cagcgccgcc gacctggcca gcgagcatag cgacaagccg
      301 atcctgatcg atgccggcta cgtcaacagc cacctcggcg agctggccga ggacgaggac
      361 ctgtcccgct acatcctttg accaccgccg ccgccacggc caacaccgtg gcggcgggcc
      421 gcgcggtccc gaaccggggt atccttgtac tccgcccttc cgcgagtccg atgcgatgcg
      481 tatcccttcc gccatccagc tgcacaaagc ctcgaagacc ctgaccctgc gctacggcga
      541 ggatagctac gacctgcctg ccgagttcct ccgcgtgcat tcgccctcgg ccgaggtcca
      601 gggccacggc aacccggtat tgcagtacgg caagctgaac gtcggcctgg tcggcgtcga
      661 acccgccggc cagtacgcac tgaagctgag cttcgacgac ggccacgaca gcggcctgtt
      721 cacctgggac tacctgtacg agctggcgac ccgcaaggac cagctatggg ccgactacct
      781 ggcggagctg gccagcgccg gcaagtcgcg cgaccccgac gagtcggtcg tcaagctgat
      841 gctctgaagc gccgggccga gtgccccttc gcggcacacg gcatccaggc gatttttctc
      901 cgctgccccg aacgagggca cgtcatttgc aacattcgcc ccgcagactg ccagaccaga
      961 catccggtcg gcgcggtcgc cacggcgaat gtcctaccct gcacggcagc aacgctctag
     1021 tccgcaggtc ggccgctcgc cgggccagcc acgccggcca ccgatgcttc tgccagcttg
     1081 cacgccagcg gaaactcgcg taaccaagac ggtgcagccc cttgtccggg ttcccttccc
     1141 tgacgtgcgt ccgccccgcc ctgaaacgtg cgggtgggct ggtagtcgag cagtagccgg
     1201 ccggacacct agtccccggt ttcgttgcgg tcccctgttt cgcctcgaac aatggagcgt
     1261 tgccgatgag tcagaagaac aataacgagc ttcccaagca agccgcggaa aacacgctga
     1321 acctgaatcc ggtgatcggc atccggggca aggacctgct cacctccgcg cgcatggtcc
     1381 tgctccaggc ggtgcgccag ccgctgcaca gcgccaggca cgtggcgcat ttcagcctgg
     1441 agctgaagaa cgtcctgctc ggccagtcgg agctacgccc aggcgatgac gaccgacgct
     1501 tttccgatcc ggcctggagc cagaatccac tgtacaagcg ctacatgcag acctacctgg
     1561 cctggcgcaa ggagctgcac agctggatca gccacagcga cctgtcgccg caggacatca
     1621 gtcgtggcca gttcgtcatc aacctgctga ccgaggcgat gtcgccgacc aacagcctga
     1681 gcaacccggc ggcggtcaag cgcttcttcg agaccggcgg caagagcctg ctggacggcc
     1741 tcggccacct ggccaaggac ctggtgaaca acggcgggat gccgagccag gtggacatgg
     1801 acgccttcga ggtgggcaag aacctggcca ccaccgaggg cgccgtggtg ttccgcaacg
     1861 acgtgctgga actgatccag taccggccga tcaccgagtc ggtgcacgaa cgcccgctgc
     1921 tggtggtgcc gccgcagatc aacaagttct acgtcttcga cctgtcgccg gacaagagcc
     1981 tggcgcgctt ctgcctgcgc aacggcgtgc agaccttcat cgtcagttgg cgcaacccga
     2041 ccaagtcgca gcgcgaatgg ggcctgacca cctatatcga ggcgctcaag gaggccatcg
     2101 aggtagtcct gtcgatcacc ggcagcaagg acctcaacct cctcggcgcc tgctccggcg
     2161 ggatcaccac cgcgaccctg gtcggccact acgtggccag cggcgagaag aaggtcaacg
     2221 ccttcaccca actggtcagc gtgctcgact tcgaactgaa tacccaggtc gcgctgttcg
     2281 ccgacgagaa gactctggag gccgccaagc gtcgttccta ccagtccggc gtgctggagg
     2341 gcaaggacat ggccaaggtg ttcgcctgga tgcgccccaa cgacctgatc tggaactact
     2401 gggtcaacaa ctacctgctc ggcaaccagc cgccggcgtt cgacatcctc tactggaaca
     2461 acgacaccac gcgcctgccc gccgcgctgc acggcgagtt cgtcgaactg ttcaagagca
     2521 acccgctgaa ccgccccggc gccctggagg tctccggcac gcccatcgac ctgaagcagg
     2581 tgacttgcga cttctactgt gtcgccggtc tgaacgacca catcaccccc tgggagtcgt
     2641 gctacaagtc ggccaggctg ctgggtggca agtgcgagtt catcctctcc aacagcggtc
     2701 acatccagag catcctcaac ccaccgggca accccaaggc acgcttcatg accaatccgg
     2761 aactgcccgc cgagcccaag gcctggctgg aacaggccgg caagcacgcc gactcgtggt
     2821 ggttgcactg gcagcaatgg ctggccgaac gctccggcaa gacccgcaag gcgcccgcca
     2881 gcctgggcaa caagacctat ccggccggcg aagccgcgcc cggaacctac gtgcatgaac
     2941 gatgaaaagc gaccagcctg aagaacagcc gcaggagcga tccgcggcct ccgccgacga
     3001 aaccccgcca gcgcctccgg cgcggccccg tgccgcgcgg aagccggcca ggccccgtat
     3061 cgccgagccc gcggctgcgc cgccgaggac cccgagcatg ccccagccct tcgtcttccg
     3121 gaccatcgac ctcgacggcc agaccatccg caccgcagtg cggccgggca aggaaggaag
     3181 cactccgctg ctgatcttca acggcatagg cgccaacctg gaactggtgt tccccttcgt
     3241 ccaggcgctc gacccggaac tggaggtgat cgccttcgac gttcccggcg tcggcggttc
     3301 ctcgacgccc agcgtgccct accgctttcc cggcctggcc aagctggcgg cgcggatgct
     3361 cgactacctg gactacggcc aggtcaacgc gatcggcgtg tcctggggcg gcgcgctggc
     3421 ccagcagttc gcccacgact atccggaacg ctgcaagaag ctgatcctcg ccgccacttc
     3481 ggctggcgcg gtgatggtgc cgggcaagcc gaaggtactg atgcgcatgg ccagcccgcg
     3541 gcgctacatc cagccctcct atggcgtaca catcgccccg gacatctacg gcggggcctt
     3601 ccgccgcgac cccaagctgg ccatggcgca tgccagcaag gtgcgttcgt cgggcaagct
     3661 gggctactac tggcaactgt tcgccgggct cggctggacc agcatccact ggctgcatag
     3721 gatccgccag ccgaccctgg tgctggccgg cgacgacgac ccgatcatcc cgctgatcaa
     3781 catgcgtgtg ctggcctggc gcattcccaa cgccgaactg cacgtgatcg acgacggcca
     3841 cctgttcctg gtgacccgcg ccgaatcggt ggcgccgatc atcatgaagt tcctcgccga
     3901 ggagcgccgt cgcgccgtca tgcaccctcg tccgttcctg cccaagaccg gctgaactgc
     3961 tgcacgggca acaccagggc agcagacact gcttcgtcat ggtgcaggtg catagtcaat
     4021 gttccgcaac ggcgcatggc gcctggcttc gccgcgacag cgcaagctct gcgacgcccc
     4081 tcccggcgtt gtggtacagc gcttgctgcc ccactgtcgg gcggcaacct ccctgttcag
     4141 gcaggtgtgc ggttctgccc cgggtgaaac ggaattcaca ggctataact gagtattccg
     4201 agccagggga cgatcgcccg tggcatccag actgtggtcc tacgacggag tgtggtccat
     4261 gcgagaaaag caggaatcgg gtagcgtgcc ggtgcccgcc gagttcatga gtgcacagag
     4321 cgccatcgtc ggcctgcgcg gcaaggacct gctgacgacg gtccgcagcc tggctgtcca
     4381 cggcctgcgc cagccgctgc acagtgcgcg gcacctggtc gccttcggag gccagttggg
     4441 caaggtgctg ctgggcgaca ccctgcacca gccgaaccca caggacgccc gcttccagga
     4501 tccatcctgg cgcctcaatc ccttctaccg gcgcaccctg caggcctacc tggcgtggca
     4561 gaaacaactg ctcgcctgga tcgacgaaag caacctggac tgcgacgatc gcgcccgcgc
     4621 ccgcttcctc gtcgccttgc tctccgacgc cgtggcaccc agcaacagcc tgatcaatcc
     4681 actggcgtta aaggaactgt tcaataccgg cgggatcagc ctgctcaatg gcgtccgcca
     4741 cctgctcgaa gacctggtgc acaacggcgg catgcccagc caggtgaaca agaccgcctt
     4801 cgagatcggt cgcaacctcg ccaccacgca aggcgcggtg gtgttccgca acgaggtgct
     4861 ggagctgatc cagtacaagc cgctgggcga gcgccagtac gccaagcccc tgctgatcgt
     4921 gccgccgcag atcaacaagt actacatctt cgacctgtcg ccggaaaaga gcttcgtcca
     4981 gtacgccctg aagaacaacc tgcaggtctt cgtcatcagt tggcgcaacc ccgacgccca
     5041 gcaccgcgaa tggggcctga gcacctatgt cgaggccctc gaccaggcca tcgaggtcag
     5101 ccgcgagatc accggcagcc gcagcgtgaa cctggccggc gcctgcgccg gcgggctcac
     5161 cgtagccgcc ttgctcggcc acctgcaggt gcgccggcaa ctgcgcaagg tcagtagcgt
     5221 cacctacctg gtcagcctgc tcgacagcca gatggaaagc ccggcgatgc tcttcgccga
     5281 cgagcagacc ctggagagca gcaagcgccg ctcctaccag catggcgtgc tggacgggcg
     5341 cgacatggcc aaggtgttcg cctggatgcg ccccaacgac ctgatctgga actactgggt
     5401 caacaactac ctgctcggca ggcagccgcc ggcgttcgac atcctctact ggaacaacga
     5461 caacacgcgg ctgcccgcgg cgttccacgg cgaactgctc gacctgttca agcacaaccc
     5521 gctgacccgc ccgggcgcgc tggaggtcag cgggaccgcg gtggacctgg gcaaggtggc
     5581 gatcgacagc ttccacgtcg ccggcatcac cgaccacatc acgccctggg acgcggtgta
     5641 tcgctcggcc ctcctgctgg gcggccagcg ccgcttcatc ctgtccaaca gcgggcacat
     5701 ccagagcatc ctcaaccctc ccggaaaccc caaggcctgc tacttcgaga acgacaagct
     5761 gagcagcgat ccacgcgcct ggtactacga cgccaagcgc gaagagggca gctggtggcc
     5821 ggtctggctg ggctggctgc aggagcgctc gggcgagctg ggcaaccctg acttcaacct
     5881 tggcagcgcc gcgcatccgc ccctcgaagc ggccccgggc acctacgtgc atatacgctg
     5941 aaagatccgg cccgggcgcc tggagccggg cacctccatc cccagaagaa gtccggatga
     6001 agacacgcga ccgaatcctc gaatgctcgc tgttgctgtt caacgaacag ggcgagccca
     6061 acgtctcgac actggagatc gccaacgaac tgggcatcag cccgggcaat ctctactacc
     6121 acttccatgg caaggaaccg ctggtgatgg cgctgttcga gcggttccag gccgagttgg
     6181 cgccgctgct cgatccgccc gaggaggtgc gcctgggcgc ggaggactac tggctgttcc
     6241 tgcacctgat cgtcgagcgc ctcgcccact accgcttcct gttccaggac ctgtccaacc
     6301 tgaccgggcg cctgccacgg ctggctcgcg gcatccgtac ctggctcggc gcgctgaagc
     6361 ggaccctggc caccctgctg gcccgcctca aggccgaccg gcagttgcgc agcgacgcgc
     6421 cggcactcgg gcaactggtc gaagagatca ccctgaccct gctgttctcc ctggattacc
     6481 agcgggtact gggcagcaag ggcgaggtgc gcacggtggt ctaccagatc atgatgctgg
     6541 tcgccccgca tctgcgcagc gaggcccaac gctcggcgga aagcctggcg cagcgctacc
     6601 tgggaccgga atgaaaaacg cccggcgagt gccgggcgtg tgccttgccg ccaggactca
     6661 gccctggctg ctcggcgtcg ccggagcgtt cgctgccgga gcgctggcag ccggtgtggc
     6721 ggcgggggca gcgggcgcgc tcgaagacgc ggcgggagcg gccggtttcg ccgcggcggg
     6781 cttggctgcc ggcttcttcg ccgcaggctt tttcgcagcc ggcttggcgg caggtttcgc
     6841 cgcgggcttg gcggcggtcg cagcggccgg cttggctgcc ggcttcgcgg caggcttggc
     6901 tgcggcaggt ttcgcagcag gcttcgccgc ggccttggct gccggcttgg tcgccggttt
     6961 cgctgcggcc ttcgcagtcg gtttggcggc gggcttggct gccggcttgg cggccgcggt
     7021 tttcgccgcg gtttttttcg ccgcgggttt cgccgcaggc ttggcggcgg ccttggctgc
     7081 cggtttagct gccggcttgg ccgctgcggt tttcgccgcg ggcttggcag ccggtttcgc
     7141 cgcaggcttg gccgctgcct tcgccgccgg cttgacgctg acgccggtga gtttctcgat
     7201 ctgcttggtc agcgtatcga ccttgctgtg cagctccttc acctcgttgc ggctcggcac
     7261 gccgagacgc gagatggcgc tgttcaggcg cttgtcgaaa gcttcctcga g
//