From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Rattus norvegicus calpain II mRNA, complete cds.
Date: 26 Jan 93 05:50:24 GMT
Distribution: bionet
Checksum: 43675 6

LOCUS       RATCALPIIA   3250 bp ss-mRNA            ROD       25-JAN-1993
DEFINITION  Rattus norvegicus calpain II mRNA, complete cds.
ACCESSION   L09120
KEYWORDS    calpain II; neutral thiol protease.
SOURCE      Rattus norvegicus (library: lambda gt10 EMBL3) cDNA to mRNA.
  ORGANISM  Rattus norvegicus
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Rodentia; Myomorpha; Muridae; Murinae.
REFERENCE   1  (bases 1 to 3250)
  AUTHORS   DeLuca,C.I., Davies,P.L., Samis,J.A. and Elce,J.S.
  TITLE     Molecular cloning and bacterial expression of cDNA for rat calpain
            II 80 kDa subunit
  JOURNAL   Unpublished (1993)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     CDS             113..2215
                     /product="calpain II"
                     /codon_start=1
                     /translation="MAGIAMKLAKDREAAEGLGSHERAIKYLNQDYETLRNECLEAGA
                     LFQDPSFPALPSSLGFKELGPYSSKTRGIEWKRPTEICADPQFIIGGATRTDICQGAL
                     GDCWLLAAIASLTLNEEILARVVPLDQSFQENYAGIFHFQFWQYGEWVEVVVDDRLPT
                     KDGELLFVHSAEGSEFWSALLEKAYAKINGCYEALSGGATTEGFEDFTGGIARWYELR
                     KPPPNLFKIIQKALEKGSLLGCSIDITSAADSEAVTYQKLVKGHAYSVTGAEEVESSG
                     SLQKLIRIRNPWGQVEWTGKWNDNCPSWNTVDPEVRANLTERQEDGEFWMSFSDFLRH
                     YSRLEIGNLTPDTLTCDSYKKWKLTKMDGNWRRGSTAGGCRNYPNTFWMNPQYLIKLE
                     EEDEDDEDGERGCTFLVGLIQKHRRRQRKMGEDMHTIGFGIYEVPEELTGQTNIHLSK
                     NFFLTTRARERSDTFINLREVLNRFKLPPGEYVLVPSTFEPHKNDDFCIRVFSEKKAD
                     YQTVDDEIEANIEEIEANEEDIGDGFRRLFAQLTGEDAEISAFELQTILRRVLAKRED
                     IKSDGFSIETCKIMVDMLDEDGSGKLGLKEFYILWTKIQKYQKIYREIDVDRSGTMNS
                     YEMRKALEEAGFKLPCQLHQVIVARFADDELIIDFDNFVRCLVRLEILFKIFKQLDPE
                     NTGTIQLDLISWLSFSVL"
BASE COUNT      878 a    788 c    868 g    716 t
ORIGIN      
        1 agctcccgca gtccgctgca gcgccccggg cttggccgcg ccccaaccga gtgctgcgcc
       61 ccgcctctcc gcgacccctc tctctgtgct gtgcgccatc ccgatcgcta ccatggcggg
      121 catcgcgatg aaactggcca aagaccgcga ggcggccgag gggctggggt ctcacgagag
      181 agccatcaag tacctcaacc aggactacga gacactgcgg aacgagtgcc tggaggccgg
      241 ggcgctcttc caggatcctt ccttccccgc cctgccgtcg tccctgggct tcaaggagtt
      301 ggggccctac tccagcaaaa ctcggggcat cgaatggaag cggcccacgg agatctgcgc
      361 agacccccag ttcattattg gaggtgctac ccgcacagac atctgccaag gagcccttgg
      421 ggactgctgg cttctggctg ccatcgcctc cctcaccttg aatgaagaga tcctggctcg
      481 agttgtgcct ctagaccaga gcttccagga aaactatgca ggcatcttcc atttccagtt
      541 ctggcaatat ggcgaatggg tggaggtggt ggtggacgac agactgccca ctaaggacgg
      601 ggagctgctc tttgttcatt cagctgaggg gagcgagttc tggagtgccc ttctggagaa
      661 ggcctatgcc aagatcaatg ggtgctatga agcactctca gggggtgcca ccactgaggg
      721 ctttgaagac ttcacaggag gcattgccag gtggtatgag ctgaggaagc ctcctcccaa
      781 tttgttcaag atcatccaga aggctttgga gaaaggttct ctgcttggct gctctatcga
      841 tatcaccagc gctgcggatt ctgaggccgt tacgtaccag aagttggtga aaggacatgc
      901 gtactccgtc actggagccg aggaggttga aagctcagga agcctgcaga aattgatacg
      961 tatcaggaac ccctggggac aagtggagtg gaccgggaag tggaatgaca actgccccag
     1021 ctggaacacg gttgacccag aagtaagggc aaatctaaca gaacggcagg aggacggaga
     1081 attctggatg tccttcagtg acttcctgag gcactactct cgtctagaaa tcggcaacct
     1141 gaccccggac accctcacct gtgactccta caagaagtgg aaactcacca agatggatgg
     1201 gaactggagg cgaggctcca ctgcaggggg ctgcaggaat tacccaaata ccttctggat
     1261 gaaccctcag tacctaatca agctggagga agaagatgaa gatgatgagg atggggagag
     1321 gggctgtacc ttcctggtgg gcctcatcca gaagcatcgg cggcggcaga ggaagatggg
     1381 tgaggacatg cacaccattg gcttcggcat ctatgaggtc ccagaggagc taacagggca
     1441 gaccaacatc cacctcagca aaaacttttt cctgacaacc cgagccaggg agcggtcaga
     1501 taccttcatc aacctccggg aggtcctcaa ccgcttcaag ctgcccccgg gagaatatgt
     1561 ccttgttcct tccaccttcg agccccacaa gaatgacgat ttctgcatcc gagtcttctc
     1621 agagaagaag gctgactacc aaactgtcga tgatgaaatc gaggccaaca ttgaagagat
     1681 tgaagccaat gaggaggaca ttggagatgg attcagaagg ctgtttgctc agctgactgg
     1741 agaggatgcg gagatctctg cctttgagct gcagaccatc ttgagaagag ttctagccaa
     1801 gcgcgaagac atcaagtcag atggcttcag catcgagacc tgtaagatca tggtggacat
     1861 gctggatgaa gatgggagcg gcaagctggg gctgaaggag ttctacatcc tctggacgaa
     1921 gattcagaaa taccaaaaaa tttaccgaga gatcgacgtg gacaggtcgg gaaccatgaa
     1981 ttcctatgag atgcggaagg ctttggaaga agcaggtttc aagctgccct gtcaacttca
     2041 tcaagtcatc gttgcccggt ttgcagatga cgaactcatc atcgactttg acaactttgt
     2101 gcggtgtttg gttcggctgg aaatactatt caagatattc aagcagctgg accctgagaa
     2161 cactggaacg atacagctcg accttatctc gtggctgagt ttttcagtac tctgaagtta
     2221 catgcctgaa gatttctggt ggaagaaaac agccaatgcc tacacttaaa tactttgtat
     2281 ctggaccttg aacgtacggg aacatttact tcatcgggtg attgtaactg aagctctaac
     2341 cgtcagaggt ggatgaactt tcacatatca gagtaatgga tctgcatacc attaaccaaa
     2401 tgcaaatgaa ttgcataacc ctcggtaagg tcagagagag ctttatgcct gtacatagta
     2461 tatcctttct acttctacac atcgtctctt catagcaata ttaaaccagg aaggactgtt
     2521 gagaggttta agtaattggg caaacagcaa ttgatctcag aggccacaat gccttgatgg
     2581 gaatatttaa agaaatttaa aaatttaaga tgcagtggtg gcatcttcca cagcaggcca
     2641 gagagaaaca gagagagaga gagagagact tggaaataca ataataaagt aatctgtgtg
     2701 gagcgtctaa agaatccagt ctgatggagg catctaaaga atccagtctg atggaggcat
     2761 ctaaagaatc caggagccag gagtatctga cacaaggcca acctgggcta cctagcaaga
     2821 cctgatctca aaaaataaat gtcaattaaa aaaaaagaag gcatgtttcc atcatttggt
     2881 tctaacaaaa gatggttttt atacaccttt gtgaacagca agccctctta gactcaagtc
     2941 cttgaccata tttggcctta gacattgaag tagtgagtag tgagatcatt tgactgacca
     3001 actgagccag gtctgccatc tgaaagaaaa tcgtgataca accccaggga tggcttacgg
     3061 ctcgggagaa cagggctcag aagatccttc ttaaaacaac caagctggct tcgtggtccg
     3121 aggaaaatgc tagcaaccat acagacatac tgccttatcc acttccaaac gtactgttaa
     3181 taaagagttg caatgcatgc acgccgtgcc ttgtttttcc tcgtagagcg tggcccccgt
     3241 ggagggtttc
//