From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: N.tabacum NTP303 pollen specific mRNA
Date: 28 Jul 92 10:08:04 GMT
Distribution: bionet
Checksum: 39659     5

LOCUS       TOBP303      1961 bp ss-RNA             PLN       28-JUL-1992
DEFINITION  N.tabacum NTP303 pollen specific mRNA
ACCESSION   X61146
KEYWORDS    germination; pollen specific.
SOURCE      Nicotiana tabacum RNA.
  ORGANISM  Nicotiana tabacum
            Eukaryota; Plantae; Embryobionta; Magnoliophyta; Magnoliopsida;
            Asteridae; Solanales; Solanaceae.
REFERENCE   1  (bases 1 to 1961)
  AUTHORS   Weterings,K.A., Reijnen,W., Van Aarssen,R., Kortstee,A.,
            Spijkers,J., Van Herpen,M., Schrauwen,J. and Wullems,G.
  TITLE     Characterization of a pollen-specific cDNA clone from Nicotiana
            tabacum expressed during microgametogenesis and germination
  JOURNAL   Plant Mol. Biol. 18, 1101-1111 (1992)
  STANDARD  full automatic
COMMENT     *source: strain=ssp. Petit Havana, SR1; *source: stage=mature
            pollen; *source: clone_library=lambda gt10; *source: clone=lambda
            303; *source: is_germline=Y;
            From EMBL    entry NTP303;  dated 29-JUN-1992.
FEATURES             Location/Qualifiers
     sig_peptide     146..208
     CDS             146..1810
                     /product="pollen specific protein"
                     /codon_start=1
                     /translation="MGSGKVTFVALLLCLSVGVIAEDPYLYFNWNVTYGTIAPLGVPQ
                     QGILINGQFPGPRINCTSNNNIVVNVFNNLDEPFLFTWNGVQHRKNSWQDGTPGTMCP
                     IMPGQNFTYRFQVKDQIGSYSYFPTTALHRAAGGYGALNVHSRALIPVPFDNPADEYN
                     VFVGDWYNKGHKTLKKILDGGRTIGRPDGIIINGKSAKVGEAKEPLFTMEAGKTYRYR
                     FCNLGMRSSVNIRFQGHPMKLVELEGSHTVQNIYDSLDLHVGQCLSVLVTADQEPKDY
                     YLVVSSRFLKQALSSVAIIRYANGKGPASPELPTPPPENTEGIAWSMNQFRSFRWNLT
                     ASAARPNPQGSYHYGQINITRTIKIFNSMSQVGGKLRYGLNGISHTNGETPLKLVEYF
                     GATNKAFKYDLMADEAPADPSKLTIATNVKNATYRNFVEIIFENHEKTIRTYHLDGYS
                     FFAVAVEPGRWSPEKRKNYNLVDGLSRNNIQVYPNSWAAIMLTFDNAGMWNLRSEMWE
                     KTYLGEQLYFSVLSPSRSLRDEYNIPDNHPLCGIVKGLSMPAPYKA"
     mat_peptide     209..1807
                     /product="pollen specific protein"
BASE COUNT      591 a    473 c    427 g    470 t
ORIGIN
        1 cgaagaagaa gaagaagaag aagcgtctcc tcttcttctt gtgagagtaa aaaagaaaac
       61 acccaaaaaa aagaaaatca tcaaaaaaac aaatttcaaa aagagttttt gtgtttgggg
      121 attaaagaat aaaaaaaaca acgtcatggg aagtggtaaa gtaacatttg tggctttgct
      181 actttgcctc tccgtagggg tgatagctga ggacccttac ctctacttta actggaatgt
      241 cacctatgga acaattgctc cattgggtgt accacaacaa ggtattctca tcaatggtca
      301 gttccccggg cctagaatta attgtacctc taacaacaac attgttgtga atgtcttcaa
      361 caatttggac gagccattcc tttttacatg gaacggtgtc caacatagga agaactcatg
      421 gcaagatggt accccgggaa ccatgtgtcc aatcatgccc ggtcaaaatt tcacctaccg
      481 tttccaggtc aaggaccaga tcggtagcta ctcctacttc ccaaccacag ccttgcaccg
      541 ggcagcgggt ggttatggtg ctctcaacgt ccacagtcgt gctctcatcc cagttccctt
      601 tgacaatcct gctgatgaat acaatgtgtt cgtcggggat tggtacaaca agggtcacaa
      661 gaccttgaaa aagatcttgg acggtggacg cactattggc aggcctgatg gtattatcat
      721 taatggtaaa tctgccaagg ttggtgaggc aaaagagcca ctctttacca tggaggccgg
      781 caagacctat aggtacagat tctgcaacct tggtatgagg tcatctgtta acatcagatt
      841 ccaaggtcac ccaatgaaat tagtcgagct agagggatcc cacaccgtac aaaacatcta
      901 cgattccttg gacctccatg ttggtcagtg cctctcagta ttggtcactg ctgatcagga
      961 gcccaaggac tactacttgg ttgtttcaag caggttcttg aagcaagccc tatcctccgt
     1021 ggccatcatc cgatacgcca acggcaaggg cccagcttct cctgagctcc caacaccccc
     1081 accagaaaac accgaaggca ttgcctggtc catgaaccag ttccgctcct tcagatggaa
     1141 cctcaccgct agtgctgccc gacccaaccc acaaggatcc taccattatg gacagatcaa
     1201 catcacccgc accatcaaga tcttcaactc aatgagccaa gtaggtggta agcttagata
     1261 tggcttgaac ggtatctccc acactaatgg cgagactcca ttgaagcttg ttgagtactt
     1321 tggagctacc aataaggcct tcaagtatga tctcatggct gacgaagccc cggccgaccc
     1381 aagcaagctc actatcgcca caaatgtgaa gaacgccacc taccgtaact tcgtggaaat
     1441 catcttcgag aaccacgaga agactatccg tacctatcac ttggatggat attccttctt
     1501 cgccgttgct gtcgagcccg ggaggtggag ccctgagaag agaaagaact acaacttggt
     1561 ggacggctta agcaggaaca acatccaagt ctatccaaac tcatgggcag ctataatgtt
     1621 gacatttgac aatgcaggta tgtggaactt gaggtcagag atgtgggaga aaacttactt
     1681 gggagagcaa ttgtacttca gtgttctctc cccaagccgc tcattgaggg atgaatacaa
     1741 catcccagac aaccatcctc tctgcggtat cgtcaagggc ctgtccatgc cagctccata
     1801 caaggcttaa attctgtaat gatcaatctg tattggtaga atcaaagggg aggggtaatg
     1861 aataggagct taatctgttc atggatgtat ttgttttctt tccccaaaaa ataaatagga
     1921 caaaacatta aataaaaaaa aaaaaaaaaa aaaaaaaaaa a
//