From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: H.sapiens topIIb mRNA for topoisomerase IIb
Date: 3 Jan 93 22:06:57 GMT
Distribution: bionet
Checksum: 08940 10

LOCUS       HUMTOPIIB    4866 bp ss-RNA             PRI       21-DEC-1992
DEFINITION  H.sapiens topIIb mRNA for topoisomerase IIb
ACCESSION   X68060
KEYWORDS    DNA topoisomerase II.
SOURCE      Homo sapiens RNA.
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chorqata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 4866)
  AUTHORS   Jenkins,J.R., Ayton,P., Jones,T., Davies,S.L., Simmons,D.L.,
            Harris,A.L., Sheer,D. and Hickson,I.D.
  TITLE     Isolation of cDNA clones encoding the beta isoenzyme of human DNA
            topoisomerase II and localisation of the gene to chromosome 3p24
  JOURNAL   Nucleic Acids Res. 20, 5587-5592 (1992)
  STANDARD  full automatic
COMMENT     *source: cell_type=EBV-transformed; *source: clone_library=NBC;
            *source: clone=pT2b-1; *source: chromosome=3p24;
               From EMBL    entry HSTOPIIB;  dated 30-NOV-1992.
FEATURES             Location/Qualifiers
     CDS             1..4866
                     /gene="topIIb"
                     /note="antitumor drug target"
                     /product="DNA topoisomerase II"
                     /codon_start=1
                     /translation="MAKSGGCGAGAGVGGGNGALTWVNNAAKKEESETANKNDSSKKL
                     SVERVYQKKTQLEHILLRPDTYIGSVEPLTQFMWVYDEDVGMNCREVTFVPGLYKIFD
                     EILVNAADNKQRDKNMTCIKVSIDPESNIISIWNNGKGIPVVEHKVEKVYVPALIFGQ
                     LLTSSNYDDDEKKVTGGRNGYGAKLCNIFSTKFTVETACKEYKHSFKQTWMNNMMKTS
                     EAKIKHFDGEDYTCITFQPDLSKFKMEKLDKDIVALMTRRAYDLAGSCRGVKVMFNGK
                     KLPVNGFRSYVDLYVKDKLDETGVALKVIHELANERWDVCLTLSEKGFQQISFVNSIA
                     TTKGGRHVDYVVDQVVGKLIEVVKKKNKAGVSVKPFQVKNHIWVFINCLIENPTFDSQ
                     TKENMTLQPKSFGSKCQLSEKFFKAASNCGIVESILNWVKFKAQTQLNKKCSSVKYSK
                     IKGIPKLDDANDAGGKHSLECTLILTEGDSAKSLAVSGLGVIGRDRYGVFPLRGKILN
                     VREASHKQIMENAEINNIIKIVGLQYKKSYDDAESLKTLRYGKIMIMTDQDQDGSHIK
                     GLLINFIHHNWPSLLKHGFLEEFITPIVKASKNKQELSFYSIPEFDEWKKHIENQKAW
                     KIKYYKGLGTSTAKEAKEYFADMERHRILFRYAGPEDDAAITLAFSKKKIDDRKEWLT
                     NFMEDRRQRRLHGLPEQFLYGTATKHLTYNDFINKELILFSNSDNERSIPSLVDGFKP
                     GQRKVLFTCFKRNDKREVKVAQLAGSVAEMSAYHHGEQALMMTIVNLAQNFVGSNNIN
                     LLQPIGQFGTRLHGGKDAASPRYIFTMLSTLARLLFPAVDDNLLKFLYDDNQRVEPEW
                     YIPIIPMVLINGAEGIGTGWACKLPNYDAREIVNNVRRMLDGLDPHPMLPNYKNFKGT
                     IQELGQNQYAVSGEIFVVDRNTVEITELPVRTWTQVYKEQVLEPMLNGTDKTPALISD
                     YKEYHTDTTVKFVVKMTEEKLAQAEAAGLHKVFKLQTTLTCNSMVLFDHMGCLKKYET
                     VQDILKEFFDLRLSYYGLRKEWLVGMLGAESTKLNNQARFILEKIQGKITIENRSKKD
                     LIQMLVQRGYESDPVKAWKEAQEKAAEEDETQNQHDDSSSDSGTPSGPDFNYILNMSL
                     WSLTKEKVEELIKQRDAKGREVNDLKRKSPSDLWKEDLAAFVEELDKVESQEREDVLA
                     GMSGKAIKGKVGKPKVKKLQLEETMPSPYGRRIIPEITAMKADASKKLLKKKKGDLDT
                     AAVKVEFDEEFSGAPVEGAGEEALTPSVPINKGPKPKREKKEPGTRVRKTPTSSGKPS
                     AKKVKKRNPWSDDESKSESDLEETEPVVIPRDSLLRRAAAERPKYTFDFSEEEDDDAD
                     DDDDDNNDLEELKVKASPITNDGEDEFVPSDGLDKDEYTFSPGKSKATPEKSLHDKKS
                     QDFGNLFSFPSYSQKSEDDSAKFDSNEEDSASVFSPSFGLKQTDKVPSKTVAAKKGKP
                     SSDTVPKPKRAPKQKKVVEAVNSDSDSEFGIPKKTTTPKGKGRGAKKRKASGSENEGD
                     YNPGRKTSKTTSKKPKKTSFDQDSDVDIFPSDFPTEPPSLPRTGRARKEVKYFTESDE
                     EEDDVDFAMFN"
BASE COUNT     1690 a    785 c   1092 g   1299 t
ORIGIN      
        1 atggccaagt cgggtggctg cggcgcggga gccggcgtgg gcggcggcaa cggggcactg
       61 acctgggtga acaatgctgc aaaaaaagaa gagtcagaaa ctgccaacaa aaatgattct
      121 tcaaagaagt tgtctgttga gagagtgtat cagaagaaga cacaacttga acacattctt
      181 cttcgtcctg atacatatat tgggtcagtg gagccattga cgcagttcat gtgggtgtat
      241 gatgaagatg taggaatgaa ttgcagggag gttacctttg tgccaggttt atacaagatc
      301 tttgatgaaa ttttggttaa tgctgctgac aataaacaga gggataagaa catgacttgt
      361 attaaagttt ctattgatcc tgaatctaac attataagca tttggaataa tgggaaaggc
      421 attccagtag tagaacacaa ggtagagaaa gtttatgttc ctgctttaat ttttggacag
      481 cttttaacat ccagtaacta tgatgatgat gagaaaaaag ttacaggtgg tcgtaatggt
      541 tatggtgcaa aactttgtaa tattttcagt acaaagttta cagtagaaac agcttgcaaa
      601 gaatacaaac acagttttaa gcagacatgg atgaataata tgatgaagac ttctgaagcc
      661 aaaattaaac attttgatgg tgaagattac acatgcataa cattccaacc agatctgtcc
      721 aaatttaaga tggaaaaact tgacaaggat attgtggccc tcatgactag aagggcatat
      781 gatttggctg gttcgtgtag aggggtcaag gtcatgttta atggaaagaa attgcctgta
      841 aatggatttc gcagttatgt agatctttat gtgaaagaca aattggatga aactggggtg
      901 gccctgaaag ttattcatga gcttgcaaat gaaagatggg atgtttgtct cacattgagt
      961 gaaaaaggat tccagcaaat cagctttgta aatagtattg caactacaaa aggtggacgg
     1021 cacgtggatt atgtggtaga tcaagttgtt ggtaaactga ttgaagtagt taagaaaaag
     1081 aacaaagctg gtgtatcagt gaaaccattt caagtaaaaa accatatatg ggtttttatt
     1141 aattgcctta ttgaaaatcc aacttttgat tctcagacta aggaaaacat gactctgcag
     1201 cccaaaagtt ttgggtctaa atgccagctg tcagaaaaat tttttaaagc agcctctaat
     1261 tgtggcattg tagaaagtat cctgaactgg gtgaaattta aggctcagac tcagctgaat
     1321 aagaagtgtt catcagtaaa atacagtaaa atcaaaggta ttcccaaact ggatgatgct
     1381 aatgatgctg gtggtaaaca ttccctggag tgtacactga tattaacaga gggagactct
     1441 gccaaatcac tggctgtgtc tggattaggt gtgattggac gagacagata cggagttttt
     1501 ccactcaggg gcaaaattct taatgtacgg gaagcttctc ataaacagat catggaaaat
     1561 gctgaaataa ataatattat taaaatagtt ggtctacaat ataagaaaag ttacgatgat
     1621 gcagaatctc tgaaaacctt acgctatgga aagattatga ttatgaccga tcaggatcaa
     1681 gatggttctc acataaaagg cctgcttatt aatttcatcc atcacaattg gccatcactt
     1741 ttgaagcatg gttttcttga agagttcatt actcctattg taaaggcaag caaaaataag
     1801 caggaacttt ccttctacag tattcctgaa tttgacgaat ggaaaaaaca tatagaaaac
     1861 cagaaagcct ggaaaataaa gtactataaa ggattgggta ctagtacagc taaagaagca
     1921 aaggaatatt ttgctgatat ggaaaggcat cgcatcttgt ttagatatgc tggtcctgaa
     1981 gatgatgctg ccattacctt ggcatttagt aagaagaaga ttgatgacag aaaagaatgg
     2041 ttaacaaatt ttatggaaga ccggagacag cgtaggctac atggcttacc agagcaattt
     2101 ttatatggta ctgcaacaaa gcatttgact tataatgatt tcatcaacaa ggaattgatt
     2161 ctcttctcaa actcagacaa tgaaagatct ataccatctc ttgttgatgg ctttaaacct
     2221 ggccagcgga aagttttatt tacctgtttc aagaggaatg ataaacgtga agtaaaagtt
     2281 gcccagttgg ctggctctgt tgctgagatg tcggcttatc atcatggaga acaagcattg
     2341 atgatgacta ttgtgaattt ggctcagaac tttgtgggaa gtaacaacat taacttgctt
     2401 cagcctattg gtcagtttgg aactcggctt catggtggca aagatgctgc aagccctcgt
     2461 tatattttca caatgttaag cactttagca aggctacttt ttcctgctgt ggatgacaac
     2521 ctccttaagt tcctttatga tgataatcaa cgtgtagagc ctgagtggta tattcctata
     2581 attcccatgg ttttaataaa tggtgctgag ggcattggta ctggatgggc ttgtaaacta
     2641 cccaactatg atgctaggga aattgtgaac aatgtcagac gaatgctaga tggcctggat
     2701 cctcatccca tgcttccaaa ctacaaaaac tttaaaggca cgattcaaga acttggtcaa
     2761 aaccagtatg cagtcagtgg tgaaatattt gtagtggaca gaaacacagt agaaattaca
     2821 gagcttccag ttagaacttg gacacaggta tataaagaac aggttttaga acctatgcta
     2881 aatggaacag ataaaacacc agcattaatt tctgattata aagaatatca tactgacaca
     2941 actgtgaaat ttgtggtgaa aatgactgaa gagaaactag cacaagcaga agctgctgga
     3001 ctgcataaag tttttaaact tcaaactact cttacttgta attccatggt actttttgat
     3061 catatgggat gtctgaagaa atatgaaact gtgcaagaca ttctgaaaga attctttgat
     3121 ttacgattaa gttattacgg tttacgtaag gagtggcttg tgggaatgtt gggagcagaa
     3181 tctacaaagc ttaacaatca agcccgtttc attttagaga agatacaagg gaaaattact
     3241 atagagaata ggtcaaagaa agatttgatt caaatgttag tccagagagg ttatgaatct
     3301 gacccagtga aagcctggaa agaagcacaa gaaaaggcag cagaagagga tgaaacacaa
     3361 aaccagcatg atgatagttc ctccgattca ggaactcctt caggcccaga ttttaattat
     3421 attttaaata tgtctctgtg gtctcttact aaagaaaaag ttgaagaact gattaaacag
     3481 agagatgcaa aagggcgaga ggtcaatgat cttaaaagaa aatctccttc agatctttgg
     3541 aaagaggatt tagcggcatt tgttgaagaa ctggataaag tggaatctca agaacgagaa
     3601 gatgttctgg ctggaatgtc tggaaaagca attaaaggta aagttggcaa acctaaggtg
     3661 aagaaactcc agttggaaga gacaatgccc tcaccttatg gcagaagaat aattcctgaa
     3721 attacagcta tgaaggcaga tgccagcaaa aagttgctga agaagaagaa gggtgatctt
     3781 gatactgcag cagtaaaagt ggaatttgat gaagaattca gtggagcacc agtagaaggt
     3841 gcaggagaag aggcattgac tccatcagtt cctataaata aaggtcccaa acctaagagg
     3901 gagaagaagg agcctggtac cagagtgaga aaaacaccta catcatctgg taaacctagt
     3961 gcaaagaaag tgaagaaacg gaatccttgg tcagatgatg aatccaagtc agaaagtgat
     4021 ttggaagaaa cagaacctgt ggttattcca agagattctt tgcttaggag agcagcagcc
     4081 gaaagaccta aatacacatt tgatttctca gaagaagagg atgatgatgc tgatgatgat
     4141 gatgatgaca ataatgattt agaggaattg aaagttaaag catctcccat aacaaatgat
     4201 ggggaagatg aatttgttcc ttcagatggg ttagataaag atgaatatac attttcacca
     4261 ggcaaatcaa aagccactcc agaaaaatct ttgcatgaca aaaaaagtca ggattttgga
     4321 aatctcttct catttccttc atattctcag aagtcagaag atgattcagc taaatttgac
     4381 agtaatgaag aagattctgc ttctgttttt tcaccatcat ttggtctgaa acagacagat
     4441 aaagttccaa gtaaaacggt agctgctaaa aagggaaaac cgtcttcaga tacagtccct
     4501 aagcccaaga gagccccaaa acagaagaaa gtagtagagg ctgtaaactc tgactcggat
     4561 tcagaatttg gcattccaaa gaagactaca acaccaaaag gtaaaggccg aggggcaaag
     4621 aaaaggaaag catctggctc tgaaaatgaa ggcgattata accctggcag gaaaacatcc
     4681 aaaacaacaa gcaagaaacc gaagaagaca tcttttgatc aggattcaga tgtggacatc
     4741 ttcccctcag acttccctac tgagccacct tctctgccac gaaccggtcg ggctaggaaa
     4801 gaagtaaaat attttacaga gtctgatgaa gaagaagatg atgttgattt tgcaatgttt
     4861 aattaa
//