From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Pseudomonas syringae copper resistance operon regulatory protein
Date: 6 Nov 92 18:34:03 GMT
Distribution: bionet
Checksum: 28012 7

LOCUS       PSECOPDRS    2388 bp ds-DNA             BCT       02-NOV-1992
DEFINITION  Pseudomonas syringae copper resistance operon regulatory protein
            genes copS, and copR genes, complete cds, and copD gene, 3' end.
ACCESSION   L05176
KEYWORDS    copD gene; copR gene; copS gene; transcriptional activator.
SOURCE      Pseudomonas syringae (pathovar tomato) DNA.
  ORGANISM  Pseudomonas syringae
            Prokaryotae; Gracilicutes; Scotobacteria; Pseudomonadaceae.
REFERENCE   1  (bases 1 to 2388)
  AUTHORS   Mellano,M.A. and Cooksey,D.A.
  TITLE     Nucleotide sequence and organization of copper resistance genes
            from Pseudomonas syringae pv. tomato
  JOURNAL   J. Bacteriol. 170, 2879-2883 (1988)
  STANDARD  full automatic
REFERENCE   2  (bases 1 to 2388)
  AUTHORS   Mills,S.D., Jasalavich,C.A. and Cooksey,D.A.
  TITLE     A two-component regulatory system required for copper-inducible
            expression of the copper resistance operon of Pseudomonas syringae
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     RBS             137..141
                     /gene="copR"
     RBS             812..815
                     /gene="copS"
     terminator      2307..2339
     CDS             <1..103
                     /citation=[2]
                     /citation=[1]
                     /note="putative"
                     /gene="copD"
                     /codon_start=2
                     /translation="IRYSMALELGAAVLVLGLIAWLGTLSPEMEAGM"
     CDS             149..832
                     /evidence="EXPERIMENTAL"
                     /citation=[2]
                     /gene="copR"
                     /product="transcriptional activator"
                     /codon_start=1
                     /translation="MKLLVAEDEPKTGIYLQQGLREAGFNVDRVVTGTDAVDQALNEA
                     YDLLILDVMMPGLDGWEVIRRLRTAGQPVPVLFLTARDGVDDRVKGLELGADDYLVKP
                     FALSELLARVRTLLRRGSSLQVQTSLQIGDLQVDLLKRRATRGGKRIELTAKEFALLE
                     LLMRRQGEVLSKSLIASQVWDMNFDSDTNVIEVAIRRLRAKIDDDFEVKLLHTCRGMG
                     YMLEAQDEG"
     CDS             829..2292
                     /note="putative sensor"
                     /evidence="EXPERIMENTAL"
                     /citation=[2]
                     /gene="copS"
                     /product="transcriptional activator"
                     /codon_start=1
                     /translation="MKPGSLTLRLSLLFVVAVAAVLIIVGVAFNELSRHHFRALDAQA
                     LGEKLEAITQIAKESGANPELLKARWHTLLGAHPDLSAVFLKTDGTPFFAEPPQSAVP
                     SLAQATQRDGVWEWEKEGRMFRALTASVSLPTASPPLTAWLVLDVTTHMHFFAMLERW
                     FWGVLLASTVLSAALGWLVAKNGLRPVARVTQTAASMSAGSLKERIPLEPVPDELRAL
                     ITAFNSMLGRLDDSFMRLSNFSADIAHELRTPISNLRTHTEVILAKKRAPEVYEENLS
                     SNLEELNRLSGIIDGMLFLAKSDNGLIVPEAVELDLRTVISKLFGYYEFLAEDKGIQL
                     QASGNASIFADSVMIDRVVSNLLSNALRYTSSGETIKVSIHDHGGRVELRLENPGPEI
                     VPQHLDRIFDRFYRVDPARREGRECGAGASDCPVLDASAWRHYLVYIPRGPNDLHPHL
                     HAIACPTNLTCRPDSLGTAKPGHTRLGEHETGCHCAG"
BASE COUNT      476 a    703 c    722 g    487 t
ORIGIN      
        1 gatccgctac agcatggccc tggaactggg cgccgccgtc ttggtgttgg gcctgattgc
       61 ctggcttggc acactgtccc ctgagatgga agcggggatg tgaccctgtt ttaccgtcac
      121 actgggccgg tgccgtggag ggtcgaacat gaaactgctg gtagccgaag acgaacctaa
      181 aactggaatc tatctgcagc aaggcctgcg ggaggccggc ttcaacgtgg accgcgtggt
      241 cacaggcaca gatgccgttg accaggcgct caatgaagcc tatgacctgt tgattctcga
      301 cgtgatgatg ccaggccttg atggctggga agtcatcaga cgccttcgca ccgctggcca
      361 gcccgtaccg gtgctgtttc tgaccgcacg ggacggggta gacgaccggg tcaagggcct
      421 ggagctgggc gccgatgatt atctggtcaa gccgtttgcc ctttcagaac tgctggcacg
      481 ggtgcgaacg ctgttacggc gcggcagcag cctgcaggtt caaacgagcc tgcaaatcgg
      541 agaccttcag gtggatttgc tcaagcgtcg ggcgactcgg ggcggcaagc gaatcgagct
      601 tacggccaaa gagtttgcac tgctggaact gctgatgcgc cggcagggtg aagtgctgtc
      661 caaatcgctg atcgcctccc aggtctggga catgaatttc gacagcgaca caaatgttat
      721 cgaggtggcg atacgacggt tgagggccaa gattgacgat gacttcgagg taaaactgct
      781 gcatacctgc cggggaatgg gttacatgct agaggcgcag gacgaaggat gaagcctggc
      841 tcactcaccc tgcgcttgag ccttctgttt gtggtcgcgg tcgccgccgt cctaatcatt
      901 gtaggggttg ccttcaacga gctgagccgt caccactttc gcgccctgga tgcgcaggcg
      961 ctgggagaaa agctggaagc gattacccag attgccaagg aatcaggcgc aaacccggaa
     1021 ctgctcaagg cgcgctggca caccctcctg ggggcacatc ccgatctcag tgctgtattt
     1081 ctgaagaccg atggaacgcc tttttttgca gaacctcccc aatcagcggt gccgtcgctc
     1141 gcgcaggcca cccagcgcga tggcgtctgg gagtgggaaa aagaaggccg catgttcagg
     1201 gccctgaccg cctcggtatc gttgcccact gccagcccgc cgctgaccgc gtggcttgtc
     1261 ctggacgtca ccacgcatat gcacttcttt gccatgcttg aacgctggtt ctggggcgtc
     1321 ctattggcta gtaccgtgtt aagcgcggcc ctggggtggc tggtcgccaa gaacgggcta
     1381 cgcccggtag ccagggtgac ccagaccgcc gcctcgatgt ccgcgggctc gctaaaggaa
     1441 cgcataccct tggagccagt gccggatgag ttgcgcgcgc tcatcaccgc gttcaattcc
     1501 atgctcggtc gcctggatga ttccttcatg cgcctatcga atttctccgc tgacatcgcg
     1561 catgagcttc gcaccccgat cagcaacctg cgcacccaca ccgaggtgat cctggccaaa
     1621 aagcgtgcgc ccgaggtgta cgaggagaat ttgagctcca acctggaaga gctcaaccgt
     1681 ctttcgggaa tcatcgacgg catgctgttc ctggccaagt ccgacaacgg gctaattgtg
     1741 cctgaggctg ttgaactgga tctgagaaca gtcatcagca agctgttcgg ctattacgaa
     1801 tttctggccg aggacaaggg gattcagcta caagcatcag gcaacgcctc gatcttcgcg
     1861 gacagcgtga tgatcgaccg tgtcgtgtcg aacctgctgt ccaatgccct gcgctatacg
     1921 tcgtccggag agacgatcaa ggtcagcatc cacgatcacg gaggaagggt agagcttcga
     1981 ttggaaaatc ctggccctga aatagtaccg cagcacctag accgcatctt tgaccggttc
     2041 taccgggtgg atccggcgcg gcgcgaaggc cgggaatgcg gggctggggc tagcgattgc
     2101 ccggtccttg atgcaagcgc atggcggcac tatctcgtgt acatcccacg agggccgaac
     2161 gaccttcatc ctcaccttca tgcgatcgcc tgccccacga acctgacatg ccggcctgac
     2221 agcctgggca ccgctaagcc aggccatacc aggcttggcg aacatgaaac cggttgtcac
     2281 tgcgccgggt agtcagccac cacctcctgc tcgccggtct ttttcagtcc gatgacctga
     2341 taggcgcttt tatgccctgt ttccatgccc ggtgagccca ttgcatgc
//