From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: B.subtilis glnRA operon, GlnR protein and glutamine synthetase (EC
Date: 10 Sep 92 10:13:56 GMT
Distribution: bionet
Checksum: 07087     7

LOCUS       BACGLNRA     3058 bp ds-DNA             BCT       10-SEP-1992
DEFINITION  B.subtilis glnRA operon, GlnR protein and glutamine synthetase (EC
            6.3.1.2).
ACCESSION   D00854
KEYWORDS    GlnR protein; glnA gene; glnR gene; glutamine synthetase.
SOURCE      B.subtilis(strain PC1219) DNA, clones pSGS(2,210).
  ORGANISM  Bacillus subtilis
            Prokaryotae; Firmicutes; Endospore-forming rods and cocci;
            Bacillaceae.
REFERENCE   1  (sites)
  AUTHORS   Nakano,Y., Tanaka,E., Kato,C., Kimura,K. and Horikoshi,K.
  TITLE     The complete nucleotide sequence of the glutamine synthetase gene
            (glnA) of Bacilus subtilis
  JOURNAL   FEMS Microbiol. Lett. 57, 81-86 (1989)
  STANDARD  full staff_review
REFERENCE   2  (bases 1 to 3058)
  AUTHORS   Nakano,Y.
  JOURNAL   Unpublished (1991)
  STANDARD  full staff_review
REFERENCE   3  (sites)
  AUTHORS   Nakano,Y. and Kimura,K.
  TITLE     Purification and characterization of a repressor for the Bacillus
            cereus glnRA operon
  JOURNAL   J. Biochem. 109, 223-228 (1991)
  STANDARD  full staff_review
COMMENT     These data kindly submitted in computer readable form by:
            Yoshio Nakano
            Laboratory of Biochemistry
            Rikkyo University
            3 Nishi-Ikebukuro
            Toshima-ku, Tokyo 171
            Japan
            Phone:  03-3985-2398
            Fax:    03-5992-3434
            [1]  sites.
            [3]  sites.
FEATURES             Location/Qualifiers
     -35_signal      845..850
     -10_signal      868..873
     RBS             891..896
                     /note="SD sequence for GlnR"
     CDS             905..1312
                     /note="GlnR"
                     /codon_start=1
                     /translation="MSDNIRRSMPLFPIGIVMQLTELSARQIRYYEENGLIFPARSEG
                     NRRLFSFHDVDKLLEIKHLIEQGVNMAGIKQILAKAEAEPEQKQNEKTKKPVKHDLSD
                     DELRQLLKNELMQAGRFQRGNTFRQGDMSRFFH"
     RBS             1354..1359
                     /note="SD sequence for GlnA"
     CDS             1371..2705
                     /note="GlnA"
                     /codon_start=1
                     /translation="MAKYTREDIVKLVKEENVKYIRLQFTDILGTIKNVEIPVSQLEK
                     ALDNKVMFDGSSIEGFVRIEESDMYLYPDLNTFVIFPWTAEKGKVARFICDIYNPDGT
                     PFEGDPRNNLKRILKEMEDLGFSDFNLGPEPEFFLFKLDEKGEPTLELNDKGGYFDLA
                     PTDLGENCRRDIVLELEEMGFEIEASHHEVAPGQHEIDFKYAGAVRSCDDIQTFKLVV
                     KTIARKHGLHATFMPKPLFGVNGSGMHCNLSLFKDGVNAFYDENADLQLSETAKHFIA
                     GIVKHATSFTAVTNPTVNSYKRLVPGYEAPCYVAWSAQNRSPLIRIPASRGISTRVEV
                     RSVDPAANPYLALSVLLAAGLDGIKNKLEAPAPIDRNIYVMSKEERMENGIVDLPATL
                     AEALEEFKSNEVMVKALGEHLFEHFIEAKEIEWDMFRTQVHPWEREQYMSQY"
BASE COUNT      956 a    645 c    674 g    783 t
ORIGIN
        1 ggatccaatc gctgcagccg gacaaatcgt cagcggcctg caatctgtgg taagccgcaa
       61 catcagctca ttgcaaaacg cggtcgtcag tattacaaaa gtacaggcgg gcacgtcgtg
      121 gaacgtgatt ccagatctcg cacaccagct tgaaattctt cagctgcttg acagactcaa
      181 caaagagcag gggcggacga ttttaatggt cattcatgat ctctcattaa aaatcccggc
      241 ggcggtcttg cgaaaacagg aggctatctc gttggaaaag cgaaatggat tgaagcttgc
      301 tcataccgta tgacgtcacc tggcatcggc agagaagcag gagcatctct ttactcgctc
      361 caagaaatgt atcaaggatt ctttttggcg cctcacgttg tgtctcaaag cttaaaggga
      421 gcggtgttta cagcgagatt ccttgaaaaa ctgggcttca cttcaaaccc gaaatgggat
      481 gcgaaaagaa cagatttgat acaatccgtt gagttttctg acagagaaaa gatgattgcc
      541 ttttgccagg ccattcaata tgcatcgccg atcaatgccc acgtgacacc ttatccagcc
      601 tacatgccgg gatacgaaga tgatgtcatt atggcagcag gcacatttat tcaaggagca
      661 agcatcgaat tatcagcaga tggtcctatc cgcccgccgt atgtagcgta tatacagggt
      721 ggattaacct atacgcatgt gaagaatgcc atattcagtg cagtggattc attgatggaa
      781 aagcaattaa tttaaatttt tttaaaattt cactgaattt gatgttaaga atccttacat
      841 cgtattgaca caaaatataa catcacctat aatgaaacta agttaagaaa aggaggaaat
      901 tgagatgagt gataatattc gccgctcaat gcctttattt ccaataggaa ttgtcatgca
      961 gttaacggag ttatcagcaa ggcaaattcg atattatgag gaaaatggac tgatatttcc
     1021 agccagaagc gaagggaata gacgtttatt ttcatttcat gatgtagata aactgttaga
     1081 aatcaagcac ctgatagaac aaggtgtaaa catggcagga attaaacaga ttctggcgaa
     1141 agcggaagcc gagccggagc aaaaacaaaa cgagaagacg aaaaaaccag tgaaacatga
     1201 tctgtccgat gacgaactga gacagctcct gaaaaacgag ctcatgcaag ccggccgttt
     1261 tcaaagaggg aatacattcc gccaaggcga catgtcccgc ttctttcatt aatccgttag
     1321 caactgtttt gctatataca tttacctttt aagaggagga gttttacgaa atggcaaagt
     1381 acactagaga agatatcgta aaattagtaa aagaagaaaa cgtgaagtat atccgtcttc
     1441 aatttactga cattcttgga acaatcaaga atgttgagat tcctgtaagt cagcttgaaa
     1501 aagcgcttga taataaagtc atgtttgacg gttcttctat tgaaggattc gttcgcatcg
     1561 aagagtcaga catgtatctg tatccagatc tgaatacatt tgttatcttc ccatggacag
     1621 ctgaaaaagg taaagtagca cgtttcatct gtgatatcta caatccggat ggcacacctt
     1681 tcgaaggtga cccgcgaaac aacttaaaac ggattttgaa agaaatggaa gacctaggct
     1741 tcagtgattt caacctcggg cctgagcctg aattcttctt attcaaatta gacgaaaaag
     1801 gcgagccgac gcttgaacta aacgacaaag gcggatactt cgacttggct ccaactgact
     1861 tgggagaaaa ctgccgccgc gatatcgttc ttgagcttga agagatgggc tttgaaatcg
     1921 aagcgtctca ccacgaagta gcgcctggcc agcacgaaat cgactttaaa tatgctggtg
     1981 cagtccgctc ttgtgacgac atccaaacgt ttaaactagt tgtcaaaaca attgcccgca
     2041 aacacggtct gcatgcgaca tttatgccaa aaccattgtt cggtgtgaac ggttccggta
     2101 tgcactgcaa tctatcactc ttcaaagatg gcgttaacgc attctatgac gaaaacgcag
     2161 accttcagtt aagtgaaaca gcgaaacact tcattgcagg tatcgtgaag cacgcaacaa
     2221 gctttacagc agtaacaaat ccgacagtaa actcttacaa acgtcttgtt cctggctatg
     2281 aagcaccttg ctacgttgca tggagcgcgc aaaacagaag cccgcttatc cgtatcccgg
     2341 cttcccgcgg catcagcaca cgtgttgaag tccgcagcgt agatccagct gctaacccgt
     2401 accttgcact tagtgtattg cttgctgcag gcttagacgg aatcaaaaac aaactagaag
     2461 cgccagctcc aatcgaccgc aacatctacg tcatgagcaa agaagagcgc atggaaaacg
     2521 gaatcgttga ccttccagca acacttgcgg aagcgctaga agaattcaaa tcaaacgaag
     2581 tcatggtcaa agcgctgggc gagcacctat tcgaacactt catcgaagca aaagaaatcg
     2641 aatgggatat gttccgcacg caggttcacc cttgggagcg cgaacagtat atgtctcaat
     2701 attaatcttt caatcccttg gcactattgg tgtcagggga ttttttgatt tagattaata
     2761 gaatgagaaa ttagattttt ggtcaatgac caaattatga ccaaagatat ttgaagttag
     2821 aaatctaact ctacgattgg cgagacgata attctttgtc cggttttggt caagtttttt
     2881 acttacatga gaataaaaat tcgcgtttac ctttatgacc taagcgctct catgtctgca
     2941 cctgattcta aaagtaatac agcatgcgtg tgaatgcatg gattggcatt ttatcaatat
     3001 ttgctcgttt taaaatgcga ttaaacgcat tgaagagagt agacttacgt aagatatc
//