From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Expression vector p-delta-blue DNA fragment.
Date: 21 Aug 92 10:12:55 GMT
Distribution: bionet
Checksum: 22743     7

LOCUS       SYNPDBLUE    3507 bp ds-DNA             SYN       21-AUG-1992
DEFINITION  Expression vector p-delta-blue DNA fragment.
ACCESSION   M88655
KEYWORDS    expression vector.
SOURCE      Cloning vector DNA.
  ORGANISM  Cloning vector
            Artificial sequences; Cloning vehicles.
REFERENCE   1  (bases 1 to 3507)
  AUTHORS   Brandt,M.E. and Vickery,L.E.
  TITLE     Expression and characterization of human mitochondrial ferredoxin
            reductase in Escherichia coli.
  JOURNAL   Arch. Biochem. Biophys. 294, 735-740 (1992)
  STANDARD  simple staff_entry
FEATURES             Location/Qualifiers
     promoter        406..466
                     /note="Bacteriophage lambda pL promoter"
     misc_feature    667..688
                     /note="5' primer used for construction"
     CDS             826..1326
                     /note="Modified lacZ'"
                     /gene="lacZ"
                     /codon_start=1
                     /translation="MVTNSSSVPGDPLESTCRHASLALAVVLQRRDWENPGVTQLNRL
                     AAHPPFASWRNSEEARTDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVV
                     VPSNWQMHGYDAPIYTNVTYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIF
                     DGVPIG"
     CDS             complement(2630..3490)
                     /product="beta-lactamase"
                     /codon_start=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
BASE COUNT      918 a    887 c    824 g    878 t
ORIGIN
        1 attatgtaag cagacagttt tattgttcat gatgatatat ttttatcttg tgcaatgtaa
       61 catcagagat tttgagacac aacgtggctt tgttgaataa atcgaacttt tgctgagttg
      121 aaggatcaga tcacgcatct tcccgacaac gcagaccgtt ccgtggcaaa gcaaaagttc
      181 aaaatcacca actggtccac ctacaacaaa gctctcatca accgtggctc cctcacttct
      241 ggctggatga tggggcgatt caggctggta tgagtcagca acaccttctt cacgaggcag
      301 acctcagcgc cggtgatgcc gacgatgcgt ccggcgtaga ggatctctca cctaccaaac
      361 aatgcccccc tgcaaaaaat aaattcatat aaaaaacata cagataacca tctgcggtga
      421 taaattatct ctggcggtgt tgacataaat accactggcg gtgatactga gcacatcagg
      481 caggacgcac tgaccaccat gaaggtgacg ctcttaaaat taagccctga agaagggcag
      541 cattcaaagc agaaggcttt ggggtgtgtg atacgaaacg aagcattggc gagctcgccc
      601 ctaaagatca acaaggccga aagatttttt taactataaa cgctgatgga ggcgtttatg
      661 cccaagaggt aaagcccttc ccgagtaaca aaaaaacaac agcataaata accccgctct
      721 tacacattcc agccctgaaa aagggcatca aattaaacca cacctatggt gtatgcattt
      781 atttgcatac attcaatcaa ttgttatcta aggaaatact tacccatggt tacgaattcg
      841 agctcggtac ccggggatcc tctagagtcg acctgcaggc atgcaagctt ggcactggcc
      901 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
      961 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
     1021 caacagttgc gcagcctgaa tggcgaatgg cgctttgcct ggtttccggc accagaagcg
     1081 gtgccggaaa gctggctgga gtgcgatctt cctgaggccg atacggtcgt cgtcccctca
     1141 aactggcaga tgcacggtta cgatgcgccc atctacacca acgtaaccta tcccattacg
     1201 gtcaatccgc cgtttgttcc cacggagaat ccgacgggtt gttactcgct cacatttaat
     1261 gttgatgaaa gctggctaca ggaaggccag acgcgaatta tttttgatgg cgttcctatt
     1321 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt
     1381 ttacaattta aatatttgct tatacaatct tcctgttttt ggggcttttc tgattatcaa
     1441 ccgcggtaca tatgatggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     1501 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     1561 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     1621 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     1681 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     1741 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     1801 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     1861 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     1921 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     1981 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     2041 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     2101 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     2161 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     2221 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     2281 agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga
     2341 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     2401 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     2461 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     2521 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     2581 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     2641 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     2701 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     2761 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     2821 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     2881 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     2941 ttgctgcagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     3001 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     3061 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     3121 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     3181 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     3241 cgtcaacacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     3301 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     3361 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     3421 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     3481 gaatactcat actcttcctt tttcaat
//