From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: S.cerevisiae protein disulfide isomerase (PDI) gene, complete cds.
Date: 22 Dec 92 22:57:47 GMT
Distribution: bionet
Checksum: 29855 5

LOCUS       YSCPDIAA     2403 bp ds-DNA             PLN       30-NOV-1992
DEFINITION  S.cerevisiae protein disulfide isomerase (PDI) gene, complete cds.
ACCESSION   M62815
KEYWORDS    protein disulfide isomerase.
SOURCE      Saccharomyces cerevisiae (strain X2180) DNA.
  ORGANISM  Saccharomyces cerevisiae
            Eukaryota; Fungi; Eumycota; Hemiascomycetes; Endomycetales;
            Saccharomycetaceae.
REFERENCE   1  (bases 1 to 2403)
  AUTHORS   LaMantia,M., Miura,T., Tachikawa,H., Kaplan,H.A., Lennarz,W.J. and
            Mizunaga,T.
  TITLE     Glycosylation site binding protein and protein disulfide isomerase
            are identical and essential for cell viability in yeast
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 88, 4453-4457 (1991)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     CDS             445..2013
                     /EC_number="5.3.4.1"
                     /gene="PDI"
                     /product="protein disulfide isomerase"
                     /codon_start=1
                     /translation="MKFSAGAVLSWSSLLLASSVFAQQEAVAPEDSLSLSWPPTLSMN
                     TFSRTTWWLAEFFAPWCGHCKNMAPEYVKAAETLVEKNITLAQIDCTENQDLCMEHNI
                     PGFPSLKIFKNSDVNNSIDYEGPRTAEAIVQFMIKQSQPAVAVVADLPAYLANETFVT
                     PVIVQSGKIDADFNATFYSMANKHFNDYDFVSAENAEDDFKLSIYLPSAMDEPVVYNG
                     KKADIADADVFEKWLQVEALPYFGEIDGSVFAQYVESGLPLGYLFYNDEEELEEYKPL
                     FTELAKKNRGLMNFVSIDARKFGRHAGNLNMKEQFPLFAIHDMTEDLKYGLPQLSEEA
                     FDELSDKIVLESKAIEPLVKDFLKGDASPIVKSQEIFENQDSSVFQLVGKNHDEIVND
                     PKKDVLVLYYAPWCGHCKRLAPTYQELADTYANATSDVLIAKLDHTENDVRGVVIEGY
                     PTIVFYPGGKKSESVVYQGSRSLDSLFDFIKENGHFDVDGKALYEEAQEKAAEEADAD
                     AELADEEDAIHDEL"
BASE COUNT      667 a    562 c    487 g    687 t
ORIGIN      
        1 aaggggttga ccgtccgtcg gcacaccact tataatgcgg ggtgcaagcc ccccgtctaa
       61 aatttttttt ttttccattt ttgtcgttat tgttatttcc cgttttttgt tttttttgat
      121 tttttcggag cgacaaacct ttcgaaacac gtgtcctgaa aattatcctg ggctgcacgt
      181 gataatatgt taccctgtcg ggcggcgcct ctttttccct tttctctcac tagtctcttt
      241 ttccaatttg ccaccgtgta gcattttgtt gtgctgttac aaccacaaca aaacgaaaaa
      301 cccgtatgga catacatata tatatatata tatatatata tatattttgt tacgcgtgca
      361 ttttcttgtt gcaagcagca tgtctaattg gtaattttaa agctgccaag ctctacataa
      421 agaaaaacat acatctatcc cgttatgaag ttttctgctg gtgccgtcct gtcatggtcc
      481 tccctgctgc tcgcctcctc tgttttcgcc caacaagagg ctgtggcccc tgaagactcc
      541 ctgtcgttaa gttggccacc gactctttca atgaatacat tcagtcgcac gacttggtgg
      601 cttgcggagt tttttgctcc atggtgtggc cactgtaaga acatggctcc tgaatacgtt
      661 aaagccgccg agactttagt tgagaaaaac attaccttgg cccagatcga ctgtactgaa
      721 aaccaggatc tgtgtatgga acacaacatt ccagggttcc caagcttgaa gattttcaaa
      781 aacagcgatg ttaacaactc gatcgattac gagggaccta gaactgccga ggccattgtc
      841 caattcatga tcaagcaaag ccaaccggct gtcgccgttg ttgctgatct accagcttac
      901 cttgctaacg agacttttgt cactccagtt atcgtccaat ccggtaagat tgacgccgac
      961 ttcaacgcca ccttttactc catggccaac aaacacttca acgactacga ctttgtctcc
     1021 gctgaaaacg cagaggatga tttcaagctt tctatttact tgccctccgc catggacgag
     1081 cctgtagtat acaacggtaa gaaagccgat atcgctgacg ctgatgtttt tgaaaaatgg
     1141 ttgcaagtgg aagccttgcc ctactttggt gaaatcgacg gttccgtttt cgcccaatac
     1201 gtcgaaagcg gtttgccttt gggttacttg ttctacaatg acgaggaaga attggaagaa
     1261 tacaagcctc tctttaccga gttggccaaa aagaacagag gtctaatgaa ctttgttagc
     1321 atcgatgcca gaaaattcgg cagacacgcc ggcaacttga acatgaagga acaattccct
     1381 ctatttgcca tccacgacat gactgaagac ttgaagtacg gtttgcctca actctctgaa
     1441 gaggcgtttg acgaattgag cgacaagatc gtgttggagt ccaaggctat tgaacctttg
     1501 gttaaggact tcttgaaagg tgatgcctcc ccaatcgtga agtcccaaga gatcttcgag
     1561 aaccaagatt cctctgtctt ccaattggtc ggtaagaacc atgacgaaat cgtcaacgac
     1621 ccaaagaagg acgttcttgt tttgtactat gccccatggt gtggtcactg taagagattg
     1681 gccccaactt accaagaact agctgatacc tacgccaacg ccacatccga cgttttgatt
     1741 gctaaactag accacactga aaacgatgtc agaggcgtcg taattgaagg ttacccaaca
     1801 atcgtcttct acccaggtgg taagaagtcc gaatctgttg tgtaccaagg ttcaagatcc
     1861 ttggactctt tattcgactt catcaaggaa aacggtcact tcgacgtcga cggtaaggcc
     1921 ttgtacgaag aagcccagga aaaggctgct gaggaagccg atgctgacgc tgaattggct
     1981 gacgaagaag atgccattca cgatgaattg taattctgat cactttggtt tttcattaaa
     2041 tagagatata taagaaattt tctaggaagt ttttttaaaa aaaatcataa aaagataaac
     2101 gttaaaattc aaacacaata gtcgttcgct atattcgtca cactgcacga acgccttagg
     2161 gaaagagaaa attgaccacg tagtaataat aagtgcatgg catcgtcttt tacttaaatg
     2221 tggacacttg ctttactgct taggaaacta cttatctcat cctcctccat tccctctcct
     2281 tttccaatta ccgtaataaa agatggctgt atttactcct ccatcaggta atagcaattc
     2341 cgaccatact cacacacaag atgaccacga caaagatgat atgatatcaa gaaattctat
     2401 aca
//