From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Shallot virus X DNA sequence, open reading frames 1 ( RNA
Date: 28 Dec 92 21:45:59 GMT
Distribution: bionet
Checksum: 15863 17

LOCUS       SLVRNAGENM   8832 bp ss-RNA             VRL       02-DEC-1992
DEFINITION  Shallot virus X DNA sequence, open reading frames 1 ( RNA
            replicase) through 5 (coat protein) and ORF 6.
ACCESSION   M97264
KEYWORDS    RNA replicase; coat protein.
SOURCE      Shallot latent virus cDNA to genomic RNA.
  ORGANISM  Shallot latent virus
            Viridae; ss-RNA nonenveloped viruses; Carlaviridae.
REFERENCE   1  (bases 1 to 8832)
  AUTHORS   Kanyuka,K., Vishnichenko,V., Levay,K., Kondrikov,D., Ryabov,E. and
            Zavriev,S.K.
  TITLE     Nucleotide sequence of shallot virus X RNA reveals a 5'-proximal
            cistrons
  JOURNAL   J. Gen. Virol. (1992) In press
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     CDS             99..5255
                     /note="ORF 1"
                     /product="replicase"
                     /codon_start=1
                     /translation="MTAVQKLFDQISDPNTKAGYSNACFEAAQRRPKKAMAIAPFSVT
                     TPEALTLERFGITTSPFATTSHTHAADKIIENDCLTIIGHYLPKREAVTLIQLKRSKI
                     HLLGRQPSQDNFQNYCHEPKDVLRYGITHPNSCPVVNTEYAVLADTLHFMSPRQLYHL
                     FSRNPKLERLFATLVLPIEAQHRLPSLFPDVYRLEYYKDHFAYMPGGHGGGAYVHSYG
                     TLKWLDTAQVGPVDYTKSSITNPWPITDYLSIEKIETKAAHHIMFIQRTRAQVDWPLP
                     PIWVYHASEYVKLPLIFYPPEANVQKTYPHTLIKRMQLYCFSVKAVSLRDIFAKLRQV
                     IETQELVRYSMADLIRLANYFLFITGMNQVSDYESPLLENLFGKMCASIRMRLRTFFQ
                     NLLGKTSYAALLTVTDVIPVHFTTQPKRREAVGELWFQEPKWSVSTMTQPRKEHHRLQ
                     MTWTLLAWFHQLESSGSMSEPCNNSESTPQRTATSQQKAAKLTTSQKHNRRTDQTTMN
                     PQYPPLMLTIAPMMPRHSLMKKTIATPCRTLEEISDLDLDDFDDLPNEASNEPPSANE
                     QSPDNHAETTTRGVFPCECGTEITVNSFGRAIEVAGVNLTDHMKGRLAAFYSRDGQGY
                     SYTGYSHKSQGWLEGLDKLIEACGEKPTTYNQCLVQKYEQGSRIGFHSDEQAIYPKGN
                     KILTVNAAGSGTFGIKCAKGETTLNLEDGDYFQMPSGFQETHKHNVVAVTPRLSFTFR
                     STVVNSQKKPAEPEKLNQNNACPKPSDPSNASGKQHKKTHPAKGNEKSSSPNLEPLDA
                     PTVEILKLHGFTALTPQHDGTCQIRPVYFNKDIHLRRKAVKTDMSPPARPFFDLATSL
                     HRGIYTHKIDNRRATAYMSDVKNNLTGLVLPKLDRDLLSSWVALAETTTREVAVLAIH
                     GAGGAGKSRALQELLRSSPELADSINIVVPTINLANDWKAKLPQMDPRRVMTFQKACE
                     RECKSVTIFDDYGKLPAGFVDAYLAIKVNVELAILTGDQRQSTHHQERESQISSLQSN
                     IAQFSKYADYYLNATHRQPRRLANPIKVHAERQLGGAVLKANIVPDLAMVLVPAFRSQ
                     SLLTDLGRHAMTYAGCQGLTLNHLTIILDKDTPLCSDEVLYTAFSRASESITFVNTHS
                     DNPAFLAKLDATPYLKTLISWVREDEEAGADCPATEPLVKDVPTKTHIPVANDKVQLE
                     GKIEAMEDKDTRELWSGEEKTNLMQTQDPVVQLFPHQQAKDEALFKITIGERIRMATP
                     EQNAKQLRHTLNAGDLLFEAYAQFMKVPKETQPFDKRLWTHCRQLALRTYLSKPTSNL
                     QQGARQDPDFPDNAIALFNKSQWVKKLEKVGARFKAGQTISAFKQEVVLLTTTMALYL
                     RKKREQHQPDNVFIMCERTPEQFNAFVMTKWDFDRPNYTSDYTQYDQSQDAAFLNFEI
                     RKARHLGVPEDVLSFYKFIKTHAKTFLGNLAIMRLSAEGPTFDANTECNIAYDALRFR
                     LGDDVRASYAGDDLVRDKACEERAGWVYSESLFSLKAKPLVTNKPDFCGWRLTRHGIV
                     KSPIQLYQSLQLALRLGKIDEVKRSYAIDYLFAYRLGDKIYDIFDEDELEKHQLVTRT
                     LIKKGMQPPESGNHLPIFHITSDRLIRDPDAVKVQSYECDRILLKQPHIIDDYIPAGT
                     QPRNTEHPASADRRDMTRACNLSAEKLAFGGNTINHLFRTSWEGRSPLSN"
     CDS             5315..6040
                     /note="ORF 2"
                     /codon_start=1
                     /translation="MKTDLLLQILSNNNFTRTSEPIKEPLIIHGVPGSGKSTLVRALV
                     TYRSTVACTLGAPYGSNLAFPGVTSPGLTQSLTDHETRILDEYQLGTESDLKPFNVLV
                     GDPFQGNLHLKAHYVKSFSHRVPRIICNFLQSLGYEIAGSKPGELAQLPIYGPNPSGP
                     TGQVLHLGPLSRRLTQSHGVCSKLPSEVQGLEFEEVTLVYHSSEFERNRVGFYIAATR
                     ALGRLNLITDTTLEIPHELCPTS"
     CDS             6018..6329
                     /note="ORF 3"
                     /codon_start=1
                     /translation="MSFAPPPDYSKIYLALGCGLGLGFVVYASRVNHLPHVGDNTHNL
                     PHGGQYCDGNKRVLYSGPKSGSSPTNNLWPFITVIALTLAILLTSCPRRRVCIRCSQH
                     H"
     CDS             6380..7522
                     /note="ORF 4"
                     /codon_start=1
                     /translation="MVIVTTFHIDAARDRIINCVKDVRNIVTNQVVPATRKLGSIETT
                     LENFRTETIGGFTTISDCVSLLRNLRSETTRNFNTLLSRTAEPTGQAQTQLRQGFDEP
                     DGHKSEQRTFFSNLDTALNATQALLNHVPPARYTLPPAPLPVNESFGQLHALHLNTLE
                     WLTHINHNLDSMLNMLNPANLMSQGTPLSRLKDAVRTLTQNMNTIQSDQQKILASTSA
                     TNHSDILRKLESLDTGLKQLGIRLDVVVSSLNNMSERPPTPSHDTASSSTSTDPNPLP
                     PYQAVHPSLFCRTYGNILYNGIDSRIPMDVTGRPASTSLKLTITVECSEQNTRVNFTL
                     LDDGYILLSDSIETKHKLQHIPSDCLSLIHARCPKFVYKFRGEGLC"
     CDS             7546..8334
                     /note="ORF 5"
                     /product="coat protein"
                     /codon_start=1
                     /translation="MNEEDLNRLNASGDLNGANNQRIPAGPSGVNQPIPSVTAGGQNQ
                     FRPSGGLGNQGSRPTESSNQDELLPTEAEIEAITSDVESNSVAPKATIREILDTLQAK
                     RQNATPKDLFSLAWACYHNGSSRFVNLNTDAPCGITHADLKTLWKASATLRQFCSYYA
                     KSCYVSGKQQKKPPANWSRKGYPEEAKFAGFDFFNAVLSESSPAPPGGMRFKPTQAEI
                     LGHSMNAKMSIVESRQSSHMVSTRADLLGRQQINEQPKPPMITF"
     CDS             8334..8720
                     /note="ORF 6"
                     /codon_start=1
                     /translation="MHPHDLNLLCCLHFSKPSLPNDLKTLLFRACETSCKLNRRLLDN
                     KPFQGTSKCAKRRRAKRYNRCFDCGAYLYDDHVCKRFTSRSNSDCLSVIHQGPAKLYA
                     EGAYRANSDAEQLIMNDMLLIKSLKL"
BASE COUNT     2528 a   2522 c   1822 g   1959 t      1 others
ORIGIN      
        1 gactcaacca aaacaacaca caaccacaca aaacgcacaa acttgacttg agtctgataa
       61 ggaaactcag caaagccctg tacacccatc aagcaagtat gactgctgtg caaaaacttt
      121 ttgaccaaat tagcgacccg aacacgaaag ctggctactc caatgcttgc tttgaagcgg
      181 cgcaacggcg tccaaagaaa gctatggcta tagccccttt ctccgtcacc accccagaag
      241 ctctcacgct tgagcgcttc ggcatcacca cctccccttt cgccaccact tcacacacac
      301 atgctgccga caaaataatt gagaatgact gcctcacaat tattggccac tatctaccca
      361 aacgagaggc agtgactctt atccaactca aacgcagcaa aattcatctt ctgggcaggc
      421 aacccagtca agacaatttc caaaattatt gccacgagcc aaaggacgta cttaggtacg
      481 ggatcaccca cccaaactct tgcccagtcg ttaacacaga atacgctgtt ctggcggata
      541 ccttacactt tatgtcccct agacagctgt accacttatt cagcagaaat ccaaagctag
      601 agcgcctctt tgccacgcta gtactaccaa ttgaagctca gcacaggcta ccgagcttat
      661 ttcctgatgt gtacagactg gaatattata aagaccattt tgcttatatg cccggaggtc
      721 acggtggcgg tgcttacgta cattcttacg gcactctcaa atggctagac acggctcaag
      781 tgggccccgt agattacact aaatcttcaa tcaccaaccc atggcccata accgactatc
      841 ttagcataga gaagattgaa accaaagcag cgcaccatat aatgttcata cagcgcacac
      901 gggctcaggt tgattggcca ctgccgccca tatgggtcta ccacgcctca gaatacgtca
      961 aattacccct catattctac cccccagagg ccaatgtaca gaagacgtac ccccacaccc
     1021 tcatcaagag aatgcaactg tattgcttct cagtcaaagc cgtatcacta agagacatct
     1081 ttgctaagct tcggcaagtc attgagacac aagaacttgt tcgctactcc atggcagacc
     1141 tcatcaggct agccaactac ttcctgttca tcacgggtat gaatcaagtc agcgactacg
     1201 aatctccact gctcgagaat ttattcggga aaatgtgtgc ttcaatccgc atgagactca
     1261 gaactttctt ccaaaacctg cttggcaaaa catcttacgc tgccctgctc acggtgacgg
     1321 atgtcattcc ggtccacttc actacccagc ccaaacgcag agaggctgtc ggcgagctat
     1381 ggtttcagga gcccaaatgg agcgtaagca cgatgaccca accccgcaaa gaacatcacc
     1441 gtctccagat gacctggacc ttgctagcgt ggtttcacca acttgagagt tcgggctcca
     1501 tgtcagaacc ctgtaataat tccgagtcca cgccacagcg cacagcaacc agccaacaga
     1561 aagcggccaa gttaacgacc tcacagaaac acaacaggcg gacggaccaa acgacgatga
     1621 acccacaata ccctccgctg atgttgacga ttgcgccgat gatgcctcga cattctctga
     1681 tgaagaagac gattgcgact ccatgccgga cgctcgaaga gatttcagat cttgacttag
     1741 atgattttga tgacctgcct aacgaagctt caaacgaacc gccgagcgct aatgagcaat
     1801 ccccggacaa tcatgctgaa acaaccacga gaggagtgtt tccttgcgag tgcggcaccg
     1861 aaatcacagt taactccttt ggtcgagcga tagaagttgc aggcgtaaat ctgaccgacc
     1921 acatgaaagg caggctcgct gccttctact cacgggatgg ccaaggctac tcttacactg
     1981 gctactcgca caaatcgcaa ggctggttag agggtcttga caagctgatc gaagcatgcg
     2041 gtgaaaaacc taccacctac aaccagtgcc tggtacagaa atacgagcaa ggctcaagaa
     2101 taggtttcca tagtgacgag caagctatat acccaaaagg caataaaatc ctcaccgtga
     2161 acgcagccgg ctccggaaca tttggcatta agtgtgcaaa aggagaaacc accctgaatc
     2221 tcgaggatgg tgactatttc caaatgccaa gcggtttcca ggaaactcac aagcataacg
     2281 tcgtggcagt tacacctcgc ttatccttca ctttcagatc gaccgtcgtg aatagccaaa
     2341 agaaacccgc agaacctgag aagctgaatc aaaacaatgc gtgtccaaaa ccctcagatc
     2401 catcaaacgc atcgggcaag cagcacaaga aaacccaccc tgccaagggc aacgagaagt
     2461 cttcttcacc aaacctcgaa cccctggacg cacctaccgt tgagattctc aagctccacg
     2521 gcttcactgc cctcactccc caacatgacg gcacatgcca aattcgccct gtttatttca
     2581 acaaagacat tcacttgagg cgaaaagcag ttaagactga catgtcccca ccagccaggc
     2641 cattctttga cttagctaca tctctccacc gaggcattta tacgcacaaa atcgacaacc
     2701 gtagagctac ggcgtacatg tctgacgtca agaataacct cactggctta gtcctcccta
     2761 aattggaccg tgacttgctg agttcttggg tagcgcttgc tgagactacc acacgcgaag
     2821 ttgccgtcct agccattcat ggagcaggag gagctggtaa gagtcgagct ctacaggaac
     2881 tgctgagatc ttccccagaa ctagccgaca gcatcaatat cgtggtacca actataaacc
     2941 tcgctaacga ctggaaagcc aagttaccac aaatggaccc tcgtagagtc atgaccttcc
     3001 aaaaagcttg tgagagagag tgcaaatcag tgacgatttt tgacgattac ggcaaacttc
     3061 ctgcaggatt tgtggacgcg taccttgcta tcaaggtgaa cgtcgagctg gcgatactga
     3121 ctggagatca acgccaatcc acccatcatc aagagcgtga atctcaaata tcgtcacttc
     3181 aaagtaacat cgcccagttt tcaaagtacg cagattatta cctcaatgcc actcataggc
     3241 agcccaggcg actcgctaac cctatcaaag tacacgctga gcggcaattg gggggggccg
     3301 tgctcaaagc caacattgtg ccagatctgg ccatggtact cgtgcctgca ttccgcagcc
     3361 agtcactttt gacggactta ggccgacacg ccatgactta cgccggctgt caggggctca
     3421 cacttaatca cttgacgatc atcctagaca aggacacccc tttatgctct gacgaggtgc
     3481 tctacacggc attttcccgt gcctccgagt ccataacctt tgtgaacacc cactctgata
     3541 accctgcttt cctggccaaa cttgatgcca ccccatacct caagactctg atatcatggg
     3601 ttagggagga tgaggaagct ggcgccgatt gtcccgctac tgaacccctc gttaaagatg
     3661 tgcccactaa gacacatata cccgtagcca atgacaaggt gcaactcgag gggaagatcg
     3721 aggccatgga ggacaaggac acccgagagc tatggtcagg agaagaaaag accaatctga
     3781 tgcaaactca ggacccggtt gtgcagctct tcccacacca acaagcaaag gacgaagctc
     3841 tgttcaaaat aacaattggt gagcgcattc gtatggcaac gcctgagcaa aacgctaagc
     3901 agctacgaca cacactcaat gccggcgatc tactctttga ggcgtacgca cagttcatga
     3961 aagtgcccaa ggaaacgcag ccctttgaca aacgtttatg gactcattgc cgtcaactag
     4021 ctctgcgcac ttacctctcg aaacctacat ctaaccttca acaaggggcc agacaggacc
     4081 ctgatttccc agacaatgcc atcgccctat ttaacaaatc gcaatgggtc aagaaacttg
     4141 agaaagttgg cgctcggttc aaagctggac aaacgatatc agcttttaaa caggaagtgg
     4201 tacttctaac aacgaccatg gcactttacc tcagaaagaa acgagagcaa caccagcccg
     4261 acaacgtttt tattatgtgt gaaagaaccc ctgaacaatt caacgctttt gtaatgacta
     4321 agtgggactt tgaccgaccc aactacactt cggactacac acaatatgac caatctcaag
     4381 acgccgcttt ccttaacttc gaaataagga aagcgcgaca tttaggtgtg cccgaagatg
     4441 tcctctcttt ctacaagttc attaagactc atgcgaagac cttcttgggc aacttggcca
     4501 ttatgcgtct cagcgccgaa gggcccacat ttgacgccaa cacagagtgc aatattgcat
     4561 atgacgccct cagattcagg cttggagacg acgttagggc ctcgtacgcc ggtgacgacc
     4621 tggtaagaga caaagcatgc gaagaacgcg ccggctgggt ttattctgag agtttattca
     4681 gtctcaaagc taaaccactc gtcacaaaca aacctgactt ctgcggctgg cgactcacca
     4741 gacatggcat tgtcaaatcg cccatccaac tctaccaatc gttgcaactg gcactcagac
     4801 ttggaaaaat tgacgaggtt aagcgaagct atgctatcga ctatctgttt gcctaccgcc
     4861 tgggcgacaa aatatacgac atcttcgacg aagacgaact agaaaaacat cagttggtga
     4921 ctcggaccct catcaagaaa ggtatgcaac ctcctgagtc gggcaaccac cttccaattt
     4981 tccacattac atctgatcga ttgattagag atccagacgc agtgaaagtc cagtcatacg
     5041 agtgcgacag gatcctactg aagcagccac acatcattga tgattatata cctgctggca
     5101 ctcaaccccg taatacggaa cacccagctt ctgcagatcg tcgagacatg accagagcgt
     5161 gcaatctatc ggctgaaaaa cttgcctttg ggggaaacac aatcaaccac ttatttcgaa
     5221 catcttggga aggtcgttcc cctctctcga actaactgtt caaccactaa ttaactaacc
     5281 ccttagcatt agttatgttt tataggtgtt tgaaatgaag actgacctcc tactacaaat
     5341 actatctaac aataatttca cacgcacgtc agaaccgatc aaagagcctc tcataataca
     5401 cggtgtgcct ggttccggca aatccacctt agtgagagca ctagttactt accgatccac
     5461 tgtcgcctgc acacttggag caccttacgg cagtaaccta gcattcccag gggtcacatc
     5521 tcctgggctc acacaatctc tgacagacca cgagaccaga atccttgacg agtaccagtt
     5581 aggcacggaa tccgacttga aaccattcaa cgttctggta ggcgatccat tccaaggtaa
     5641 cctgcacctc aaggcccact acgtcaagag cttttcacat cgtgtaccaa gaatcatttg
     5701 caactttcta cagtcactcg ggtacgaaat tgcgggatcc aaacccggcg agctcgcaca
     5761 actaccaata tacggcccca acccttctgg accaaccggc caagtccttc acctaggacc
     5821 tctctcccgc aggctcactc aaagccacgg tgtctgctcc aaactcccat ctgaagttca
     5881 aggactcgag ttcgaagaag tcactctcgt gtatcactcc tctgagtttg agcgcaaccg
     5941 cgtaggtttc tacattgccg ccactcgagc gcttggaaga ctaaatctaa tcaccgacac
     6001 aacattggag atcccacatg agctttgccc cacctcctga ctactcaaag atttacctag
     6061 ccctaggctg tgggctcggt ctcggattcg ttgtctacgc ttctcgggtc aaccacctac
     6121 cacacgtcgg cgacaacact cacaatctgc cccacggagg ccagtactgt gacggcaaca
     6181 agcgtgtgct ctactcagga cccaaatctg gatcatcacc aacaaacaac ctctggcctt
     6241 tcattacggt tattgcgctc accctggcta tacttctcac tagttgccct cgccgccgtg
     6301 tttgcatacg ctgttctcaa catcattaac gggacgactc aagggtgcct tattaccatc
     6361 tcattcgatt ggagcaagca tggtaattgt cacgaccttc cacattgacg cagcacgaga
     6421 ccgcatcata aattgtgtta aagatgtcag gaacattgtc actaatcaag tcgtgcctgc
     6481 cactcggaaa ttgggatcca tagagaccac attagagaat ttcagaacag aaaccatcgg
     6541 tgggttcaca accatctccg actgtgtgtc tctactcagg aatttaagat ccgagactac
     6601 ccgcaacttc aacacattac tcagccgcac tgctgaacca acaggccaag cacaaaccca
     6661 actgcgtcaa ggctttgatg agccagacgg ccacaagagc gagcaaagaa ctttcttctc
     6721 aaatttggat acagccctga atgccacaca agcgcttctt aaccacgtac ccccggctag
     6781 atacacgcta cctccagcac cgttgcccgt taatgagtcg tttggacaat tgcacgcctt
     6841 gcacctgaac acattggaat ggctgacgca catcaatcat aacttggact ccatgcttaa
     6901 catgctcaac cctgcgaatc taatgtccca aggcactcca ctcagccgtt tgaaagacgc
     6961 agtccgtact ctcacacaaa acatgaatac tatccagtcg gatcaacaga agattctagc
     7021 gtccacttca gctactaacc actcagacat tctgcgcaag ttggaatcat tagatacagg
     7081 cctaaagcaa ctcggaatca ggctagacgt agttgtgtca agcctcaaca acatgagcga
     7141 acgaccgccc actccttcac atgacaccgc aagctcatcc acatcaacag acccaaatcc
     7201 gttaccacca taccaagcgg ttcaccccag tctattctgc cgtacctatg gcaatatcct
     7261 ctacaacggt atcgactctc gcattccgat ggatgtgacg ggccggccag ccagcacttc
     7321 ccttaaatta accattactg ttgaatgtag cgaacaaaac acaagagtta acttcacgct
     7381 acttgacgat ggatacatcc tgctgtctga ttccattgag accaaacaca aacttcaaca
     7441 catcccaagc gattgtttat ccttaataca tgccagatgc cccaaatttg tttacaaatt
     7501 taggggtgaa ggtctgtgtt aagttgtcgg gtagctcgaa atacaatgaa cgaagaggat
     7561 ttgaatcgtt tgaatgcatc aggggacctc aacggcgcca ataatcaacg aatcccggcg
     7621 ggaccatccg gcgtcaacca gccaatcccg agcgtaaccg caggtggtca aaatcaattc
     7681 agaccctcgg gaggacttgg caaccaaggc tcacgcccca ctgaatcgag taatcaggat
     7741 gaactattgc ccactgaagc tgagattgaa gccatcacta gcgacgtgga gtccaattcc
     7801 gtagcaccca aagccaccat tcgcgaaatc ctggacacct tacaagctaa gaggcaaaac
     7861 gccaccccga aagatctatt ctcattagcc tgggcctgct accacaatgg atcctcaagg
     7921 ttcgtgaact tgaatactga cgccccttgc ggaataacgc acgcagactt aaagaccttg
     7981 tggaaggctt ctgcgacgct cagacagttc tgcagctact acgctaaatc atgctatgtt
     8041 tcagggaaac agcagaagaa acctcctgct aactggtctc gaaaaggata tcctgaagag
     8101 gccaagtttg ccggctttga tttcttcaac gcagtgctga gtgagtcttc cccagctcca
     8161 ccgggcggaa tgcggttcaa acccacgcaa gctgaaattc tcggccactc aatgaacgct
     8221 aaaatgtcca tagtcgagtc ccgccaatca tcgcacatgg tttcaactag agccgacctc
     8281 ctaggccgcc aacagattaa cgaacaaccc aagccgccga tgataacttt ctgatgcatc
     8341 cccacgatct aaatcttctt tgctgcctac atttctcgaa accttccttg cccaacgacc
     8401 tcaaaactct ccttttccgt gcttgtgaga ctagctgtaa attaaaccgt agattattag
     8461 acaataagcc tttccaaggc acctctaagt gtgctaaacg ccgcagagca aaacgttata
     8521 atagatgttt tgactgtggt gcctacttat atgatgatca cgtgtgcaaa cgctttacga
     8581 gtcgctctaa ttctgactgt ttgagtgtca tccatcaggg ccctgctaag ctatatgctg
     8641 aaggggctta ccgagcaaac tcagacgccg aacagttaat tatgaacgac atgctattaa
     8701 ttaaatctct taaattataa ggcttggccc aagcctccca ctgggtttac agggctctgg
     8761 acgtgaccaa agacactcac gattgttgat atagctaaac ttactctgct acaacatttg
     8821 tccccgcgac an
//