From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Escherichia coli ibpA and ibpB genes, complete cds.
Date: 5 Jan 93 06:46:25 GMT
Distribution: bionet
Checksum: 02169 7

LOCUS       ECOPROTS     2886 bp ds-DNA             BCT       04-JAN-1993
DEFINITION  Escherichia coli ibpA and ibpB genes, complete cds.
ACCESSION   M94104
KEYWORDS    ibpA gene; ibpB gene.
SOURCE      Escherichia coli (strain W3310) DNA.
  ORGANISM  Escherichia coli
            Prokaryotae; Gram-negative facultatively anaerobic rods;
            Enterobacteriaceae.
REFERENCE   1  (bases 1 to 2886)
  AUTHORS   Allen,S.P., Polazzi,J.O., Gierse,J. and Easton,A.M.
  TITLE     Two novel heat shock genes encoding proteins produced in response
            to heterologous protein expression in Escherichia coli
  JOURNAL   J. Bacteriol. 174, 6938-6947 (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     -35_signal      539..545
                     /gene="ibpA"
                     /note="putative"
     -10_signal      560..566
                     /gene="ibpA"
                     /note="putative"
     RBS             656..660
                     /gene="ibpA"
                     /note="putative"
     RBS             1181..1186
                     /gene="ibpB"
                     /note="putative"
     stem_loop       1639..1659
                     /note="putative"
     terminator      1639..1666
                     /note="putative"
     -35_signal      1769..1774
                     /note="putative"
     -10_signal      1791..1796
                     /note="putative"
     RBS             1805..1809
                     /note="putative"
     CDS             670..1083
                     /gene="ibpA"
                     /note="putative"
                     /codon_start=1
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     CDS             1195..1623
                     /gene="ibpB"
                     /note="putative"
                     /codon_start=1
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     CDS             1819..2862
                     /note="putative;  ORF"
                     /codon_start=1
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQRTDRNAKLL"
BASE COUNT      693 a    701 c    722 g    770 t
ORIGIN      
        1 cagctgtgga tcaccgaaac tgatcgctaa atcagttatc aggcattctg gccggtggaa
       61 taaccgcatt ggtggcttgc gcgttggctt cgcttttttc aacgcgcgag cgcaccgagc
      121 tgtctttgcg gaacacatcc cacggcagta aaagcgtgtc catcacagcg gtaaacggca
      181 tatcgagaat cgccagcgac ttagtgcccc agttggtttc atcgtcgcca atcatggttg
      241 cgctggcgcg ggtgccggga tacgttcctt ctttccctcc ggtatgggac atcacgctgg
      301 agcatcctcc gagtaatgtc attccgctgc atatcatgaa cgctaacaac acatttctta
      361 tcataataaa ttcatctgtt gatcgtgggt gttggcctga tgagttatag cgatcccttg
      421 ctgaaaataa catcatcatt acgtcgcact gtggcggcta tcgcacttta acgtttcgtg
      481 ctgccccctc agtctatgca atagaccata aactgcaaaa aaaagtccgc tgataaggct
      541 tgaaaagttc atttccagac ccatttttac atcgtagccg atgaggacgc gcctgatggg
      601 tgttctggct acctgacctg tccattgtgg aaggtcttac attctcgctg atttcaggag
      661 ctattgatta tgcgtaactt tgatttatcc ccgctttacc gttctgctat tggatttgac
      721 cgtttgttta accacttaga aaacaaccag agccagagta atggcggcta ccctccgtat
      781 aacgttgaac tggtagacga aaaccattac cgcattgcta tcgctgtggc tggttttgct
      841 gagagcgaac tggaaattac cgcccaggat aatctgctgg tggtgaaagg tgctcacgcc
      901 gacgaacaaa aagagcgcac ctatctgtac cagggcatcg ctgaacgcaa ctttgaacgc
      961 aaattccagt tagctgagaa cattcatgtt cgtggtgcta acctggtaaa tggtttgctg
     1021 tatatcgatc tcgaacgcgt gattccggaa gcgaaaaaac cgcgccgtat cgaaatcaac
     1081 taattcccta aggccgcctg gcgcggcctg acatctccat gctcgccgtc agggagcata
     1141 tgcgaatctt cggatttgca ggtacttact cgcttcttag aaggagaaat gactatgcgt
     1201 aacttcgatt tatccccact gatgcgtcaa tggatcggtt ttgacaaact ggccaacgca
     1261 ctgcaaaacg ccggtgaaag ccagagcttc ccgccgtaca acattgagaa aagcgacgat
     1321 aaccactacc gcattaccct tgcgctggca ggtttccgtc aggaagattt agagattcaa
     1381 ctggaaggta cgcgcctgag cgtaaaaggc acgccggagc agccaaaaga agagaaaaaa
     1441 tggctgcatc aagggcttat gaatcagcca tttagcctga gctttacgct ggctgaaaat
     1501 atggaagtct ctggcgcaac cttcgtaaac ggtttactgc atattgattt aattcgtaat
     1561 gagcctgaac ccatcgcagc gcagcgtatc gctatcagcg aacgtcccgc gttaaatagc
     1621 taactagcta ttctctttgc cccgccattc tggtggggct ttttttgtct tacttactca
     1681 acaatgcgtt aaatttcgac tgtttaagat atttcggcac gttctttccc gtgataatgt
     1741 gcgctataag gcaaatctgc ttcaccgatt cagccataat ccgtagcaat taaaatgtta
     1801 tctcaagaga aatggacgat gagtgatata gcattaacgg tcagtattct ggctttggtg
     1861 gcagtcgtcg gtttgtttat cggcaacgtc aaatttcgcg gcataggatt aggtattggc
     1921 ggcgtgctgt ttggtgggat catcgtcggc cattttgttt ctcaggcggg gatgacatta
     1981 agtagcgata tgctgcatgt tattcaggaa tttggcctga tcctgttcgt ttatactatc
     2041 gggattcagg tagggccggg cttctttgcc tcattgcgcg tctccggatt acgcctcaac
     2101 ctgtttgctg ttctgatcgt catcatcggt ggtctggtta ccgccatcct gcataaactg
     2161 tttgatattc cactgccggt agtgctgggg attttctccg gtgcggttac caatacgcca
     2221 gcgctggggg cagggcagca gattttgcgc gacctgggta caccaatgga aatggtcgat
     2281 cagatgggga tgagttacgc gatggcgtat ccattcggca tttgcgggat tttgttcacc
     2341 atgtggatgt tgcgggttat tttccgcgtc aatgtcgaga cagaagctca gcagcacgag
     2401 tcttcacgca ccaatggcgg cgcgctgatc aagactatca atattcgcgt tgagaaccct
     2461 aacctgcatg atttagccat taaagatgta ccgattctca acggcgacaa aattatctgc
     2521 tcgcgtctga aacgcgaaga aaccctaaaa gttccttcgc cagataccat tatccaactg
     2581 ggcgatttgc tgcatctggt gggtcagcca gcggatttac ataatgcgca actggtgatt
     2641 ggtcaggagg tcgatacttc gctgtccacg aaaggcactg atttgcgcgt cgagcgtgtg
     2701 gtggtcacca atgaaaacgt gctcggaaaa cgtattcgcg acctgcactt taaagaacgc
     2761 tatgacgttg ttatctcgcg cctgaaccgt gccggggtcg aactggtcgc cagtggcgat
     2821 atcagcctgc agcgaactga tcgcaatgct aagttgctgt aacaccgggt tatgcaccga
     2881 ctgcag
//