From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: E.coli proS mRNA transfer RNA synthetase (ProRS)
Date: 3 Jan 93 22:12:58 GMT
Distribution: bionet
Checksum: 11755 6

LOCUS       ECOPRORSA    2795 bp ds-DNA             BCT       23-DEC-1992
DEFINITION  E.coli proS mRNA transfer RNA synthetase (ProRS)
ACCESSION   X55518
KEYWORDS    proS gene; transfer RNA synthetase.
SOURCE      Escherichia coli DNA.
  ORGANISM  Escherichia coli
            Prokaryotae; Gram-negative facultatively anaerobic rods;
            Enterobacteriaceae.
REFERENCE   1  (bases 1 to 2795)
  AUTHORS   Eriani,G., Delarue,M., Poch,O., Gangloff,J. and Moras,D.
  TITLE     Partition of tRNA synthetases into two classes based on mutually
            exclusive sets of sequence motifs.
  JOURNAL   Nature 347, 203-206 (1990)
  STANDARD  full automatic
COMMENT     SWISS-PROT; P16659; SYP_ECOLI.
               From EMBL    entry ECPRORSA;  dated 11-DEC-1992.
FEATURES             Location/Qualifiers
     -35_signal      552..560
                     /gene="proS"
                     /note="putative"
     -10_signal      573..581
                     /gene="proS"
                     /note="putative"
     mRNA            646..>2795
                     /gene="proS"
     terminator      2386..2397
                     /gene="proS"
                     /note="putative"
     CDS             646..2364
                     /standard_name="transfer RNAsynthetase"
                     /gene="proS"
                     /product="ProRS"
                     /codon_start=1
                     /translation="MRTSQYLLSTLKETPADAEVISHQLIVRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGQASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPQVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGRLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
BASE COUNT      663 a    727 c    755 g    650 t
ORIGIN      
        1 gatccttttc gtctttcatc aaacgatgga aggcggctgg cgtccgactg tgcgtccacc
       61 gcgcctcggc ggtaacgcca gaatgggggt tttcgcaaca cgctctactt tccgccctaa
      121 cccaattggc atgtcgctgg tagagctgaa agaggttgtt tgccataaag acagcgtgat
      181 tctgaagctc ggcagtctgg atctggtcga tggtacgccg gtagtggata tcaaaccgta
      241 tctccccttt gccgaatcgc ttcccgatgc cagtgccagc tatgcgcaaa gtgcgcagct
      301 gcagagatgg cggtgagttt taccgcagag gtcgaaaagc agcttttgac gctggagaag
      361 cgttatccgc agttaacgct gtttatccgc gaagtactgg cgcaggaccc gcgcccggcc
      421 tatcgtaaag gtgaggaaac gggcaaaacc tatgccgtct ggctacatga ttttaacgtt
      481 cgctggcgcg tcaccgacgc aggttttgaa gtctttgcgc tggaaccgcg ttaaattcac
      541 gcccttctct tttgacattt cttttgcact ggtaaactaa atcacttttt tttgtcccag
      601 gctcgccttg agcctgttct accttccaac tggaaccgta acaacatgcg tactagccaa
      661 tacctgctct ccactctcaa ggagacacct gccgacgccg aggtgatcag ccatcagctg
      721 atcgtgcgcg ccgggatgat ccgcaagctg gcctccgggt tatatacctg gctgccgacc
      781 ggcgtgcgcg ttctgaaaaa agtcgaaaac atcgtgcgtg aagagatgaa caacgccggt
      841 gcgatcgagg tgtcgatgcc ggtggttcag ccagccgatt tgtggcaaga gagtggtcgt
      901 tgggaacagt acggtccgga actgctgcgt tttgttgacc gtggcgagcg tccgttcgta
      961 ctcggcccaa ctcatgaaga agttatcact gacctgattc gtaacgagct tagctcttac
     1021 aaacagctgc cgctgaactt ctatcagatc cagaccaagt tccgcgacga agtgcgtccg
     1081 cgtttcggcg tcatgcgttc ccgcgaattc ctgatgaaag atgcttactc tttccatact
     1141 tctcaggaat ccctgcagga aacctacgat gcaatgtatg cggcctacag caaaatcttc
     1201 agccgcatgg ggctggattt ccgcgccgta caagccgaca ccggttctat cggcggccag
     1261 gcctctcacg aattccaggt gctggcgcag agcggtgaag acgatgtggt cttctccgac
     1321 acctctgact atgcagcgaa cattgaactg gcagaagcta tcgcgccgaa agaaccgcgc
     1381 gctgctgcta cccaggaaat gacgctggtt gatacgccga acgcgaaaac catcgcggaa
     1441 ctggttgaac agttcaatct gccgattgag aaaacggtta agactctgct ggttaaagcg
     1501 gttgaaggca gcagcttccc gcaggttgcg ctgctggtgc gcggtgatca cgagctgaac
     1561 gaagttaaag cagaaaaact gccgcaggtt gcaagcccgc tgactttcgc gaccgaagaa
     1621 gaaattcgtg ccgtggttaa agccggtccg ggttcactgg gtccggtaaa catgccgatt
     1681 ccggtggtga ttgaccgtac cgttgcggcg atgagtgatt tcgctgctgg tgctaacatc
     1741 gatggtaaac actacttcgg catcaactgg gatcgcgatg tcgctacccc ggaagttgca
     1801 gatatccgta acgtggtggc tggcgatcca agcccggatg gccagggtag gctgctgatc
     1861 aaacgtggta tcgaagttgg tcacatcttc cagctgggta ccaagtactc cgaagcactg
     1921 aaagcctccg tacagggtga agatggccgt aaccaaatcc tgacgatggg ttgctacggt
     1981 atcggggtaa cgcgtgtggt agctgcggcg attgagcaga actacgacga acgaggcatc
     2041 gtatggcctg acgctatcgc gccgttccag gtggcgattc tgccgatgaa catgcacaaa
     2101 tccttccgcg tacaagagct tgctgagaaa ctgtacagcg aactgcgtgc acaaggtatc
     2161 gaagtgctgc tggatgaccg caaagagcgt ccgggcgtga tgtttgctga tatggaactg
     2221 atcggtattc cgcacactat tgtgctgggc gaccgtaacc tcgacaacga cgatatcgaa
     2281 tataaatatc gtcgcaacgg cgagaaacag ttaattaaga ctggtgacat cgtcgaatat
     2341 ctggtgaaac agattaaagg ctgatgccag aaagggtcct gaatttcagg gccctttttt
     2401 tacatggatt gtataaaatg gataaaccaa aggcgtactg ccgcctgttt ctccccagct
     2461 ttcttttact ttcggcttgt accgttgata tcagccagcc agacccatca gccacagcag
     2521 tagatgccga ggcaaaaaca tgggctgtca aattccagca tcaaagctct ttcaccgaac
     2581 aatcaataaa agaaataacg gcacctgatc tcaaacccgg cgatttgctg ttctcctcaa
     2641 gccttggggt aacctcattt ggaatccgcg tcttcagcac ttcctctgtg agtcacgttg
     2701 caatctttct gggtgataat aacgttgcag aagcgacagg cgctggcgtc cagatcgttt
     2761 cccttaaaaa agcgatgaag catagtgata agctt
//