From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Shewanella putrefaciens flavocytochrome c gene, complete cds; ORF 1
Date: 21 Dec 92 20:36:05 GMT
Distribution: bionet
Checksum: 39773 7

LOCUS       SHEFLAVCA    3323 bp ds-DNA             BCT       19-NOV-1992
DEFINITION  Shewanella putrefaciens flavocytochrome c gene, complete cds; ORF 1
            gene, putative cds; ORF 2 gene, 5' end cds.
ACCESSION   L04283
KEYWORDS    flavocytochrome c; fumarate reductase; periplasmic protein;
            soluble protein; tetraheme flavoenzyme.
SOURCE      Shewanella putrefaciens (strain NCMB400) DNA.
  ORGANISM  Shewanella putrefaciens
            Prokaryotae; Gram-negative bacteria.
REFERENCE   1  (bases 1 to 3323)
  AUTHORS   Reid,G.A., Chapman,S.K., Ward,F.B., Manson,F.D., Black,A.C. and
            Pealing,S.L.
  TITLE     The sequence of the gene encoding flavocytochrome c from Shewanella
            putrefaciens: A tetraheme flavoenzyme that is a soluble fumarate
            reductase related to the membrane bound enzymes from other bacteria
  JOURNAL   Biochemistry (1992) In press
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     CDS             <1..>368
                     /note="ORF 1"
                     /note="putative"
                     /codon_start=1
                     /partial
     sig_peptide     774..848
                     /codon_start=1
     mat_peptide     849..2561
                     /product="flavocytochrome c"
                     /codon_start=1
     CDS             774..2564
                     /standard_name="fumarate reductase"
                     /product="flavocytochrome c"
                     /codon_start=1
                     /translation="MKKMNLAVCIATLMGTAGLMGTAVAADNLAEFHVQNQECDSCHT
                     PDGELSNDSLTYENTQCVSCHGTLAEVAETTKHEHYNAHASHFPGEVACTSCHSAHEK
                     SMVYCDSCHSFDFNMPYAKKWLRDEPTIAELAKDKSERQAALASAPHDTVDVVVVGSG
                     GAGFSAAISATDSGAKVILIEKEPVIGGNAKLAAGGMNAAWTDQQKAKKITDSPELMF
                     EDTMKGGQNINDPALVKVLSSHSKDSVDWMTAMGADLTDVGMMGGASVNRAHRPTGGA
                     GVGAHVVQVLYDNAVKRNIDLRMNTRGIEVLKDDKGTVKGILVKGMYKGYYWVKADAV
                     ILATGGFAKNNERVAKLDPSLKGFISTNQPGAVGDGLDVAENAGGALKDMQYIQAHPT
                     LSVKGGVMVTEAVRGNGAILVNREGKRFVNEITTRDKASAAILAQTGKSAYLIFDDSV
                     RKSLSKIDKYIGLGVAPTADSLVKLGKMEGIDGKALTETVARYNSLVSSGKDTDFERP
                     NLPRALNEGNYYAIEVTPGVHHTMGGVMIDTKAEVMNAKKQVIPGLYGAGEVTGGVHG
                     ANRLGGNAISDIITFGRLAGEEAAKYSKKN"
     CDS             2968..>3323
                     /note="ORF 2"
                     /note="putative"
                     /product="fumarate reductase"
                     /codon_start=1
                     /translation="MKQSIVHIALVVNDYDEAIDFYVNKLKFDLIEDTYQAEQDKRWV
                     VVSPPGSNGVSLLLARASKPEQHDFIGNQAGGRVFLFLNTDDFWRDYNRMQLDGIKFV
                     RPPQEQDYGTVAVFEDL"
BASE COUNT      966 a    667 c    745 g    945 t
ORIGIN      
        1 agatctgccc aattgtttat taacgcctta ccctgcaagg tataagcttt agcgtgggtt
       61 gcatatatag gatcagattg cgctaactta cgatccatgt tcaccgggct aaccacagca
      121 atcacatcga tatcgctgtc aggatcatga acatctttta ccaattgttg aaaactcttt
      181 cgacgtacaa taatgcgctt attggtcagc tggccaattc tatccatcaa ctcgatatta
      241 aagccttgat caacgccgtt attccgccac tctaatggcg ctgtttttga atgtacgcca
      301 aagacaatgc tgtcttgcgc gagcacaggc atagtgatga tgataagtaa taacaataga
      361 gttttcatgc cgcgatgtta cccatttttt ccacttgaaa ctgtgcagca ttacccgaat
      421 tagcaaatca ttatgtacgt tttcttatga tgttaactcg atgtgatcta tctccacata
      481 agctatgcaa aattgcatat acctctttat aactatgcat gtttgcatag cgcattttta
      541 ggcttgtgat tgagttgtca tttctgccct ctattctggt tatctggttg ttgattgcgc
      601 ccccttaggc aaagattaca ttgagcgaaa ataatagctg atcaactcga tgctaatttg
      661 gaccgtattt ttaaagtgaa gtgaagtcag tttcacttca gcctaactaa aacaataaga
      721 ggcttctata gttaatgaat aaaccgtcat tacgtataga agggggagca aaaatgaaaa
      781 agatgaatct tgcagtctgt attgctacat taatgggcac agcaggccta atgggcactg
      841 ctgttgcggc tgataactta gctgaattcc atgtacaaaa ccaagaatgt gatagctgcc
      901 atacaccaga tggtgaactg tcaaacgaca gcttaaccta tgaaaatacc caatgcgtat
      961 cttgccatgg cacacttgct gaagtagctg aaaccacaaa acatgaacat tataatgctc
     1021 atgcttctca tttccctggc gaagtagctt gtacctcatg ccacagcgca cacgaaaaat
     1081 cgatggtgta ttgtgactct tgccacagct tcgatttcaa catgccttat gctaaaaaat
     1141 ggctacgtga cgagccgact attgctgaat tggccaaaga caaatcagaa cgtcaggctg
     1201 ctcttgctag cgcacctcac gatactgttg acgtagtggt tgtcggttct ggcggcgcag
     1261 gtttctcagc ggcaatatca gcaacagaca gtggtgctaa agtcattctt attgaaaaag
     1321 agcctgttat tggtggtaat gctaagttag ctgcgggtgg catgaacgct gcttggactg
     1381 atcaacaaaa agccaaaaaa attactgaca gcccagagtt aatgttcgaa gacaccatga
     1441 aaggtggcca aaacataaat gaccctgcat tagttaaagt attaagctca cactctaaag
     1501 actctgttga ttggatgacc gctatgggtg ccgatttaac tgatgttggc atgatgggtg
     1561 gcgcatctgt taatcgtgcg catcgtccaa ccggtggtgc gggtgttggt gctcatgttg
     1621 ttcaagtact ttatgataat gcagtgaaac gcaatatcga cttacgcatg aacactcgcg
     1681 gcattgaagt gcttaaagat gataaaggca ctgttaaagg tattctggtt aaggggatgt
     1741 acaaaggtta ctactgggtg aaagccgatg cggtaatctt agcaacgggt ggtttcgcta
     1801 aaaataacga gcgtgtcgca aagcttgatc cttcactaaa aggctttatc tctactaacc
     1861 aacctggtgc agtaggtgat ggactggatg tagctgaaaa tgcgggtggc gcattgaaag
     1921 acatgcagta tatccaagct cacccaacac tatctgttaa aggtggcgta atggtcactg
     1981 aagcggtacg tggtaatggt gcgattttgg ttaaccgtga aggtaagcgt ttcgttaacg
     2041 aaattactac tcgtgataaa gcatctgccg ctatcttagc gcaaaccggt aaatcagctt
     2101 atttgatttt tgatgattct gtgcgtaagt cactgtcaaa aattgataag tatattggtt
     2161 taggtgttgc accaacggca gatagcctag ttaaattagg taaaatggaa ggtattgacg
     2221 gcaaagcact gactgaaact gtcgcgcgtt acaacagctt agtgagtagc ggtaaagaca
     2281 ctgattttga gcgtccaaac ctaccgcgcg cacttaacga aggtaactac tatgcaattg
     2341 aagttacacc tggtgttcac cacactatgg gtggcgtgat gatcgacact aaagctgaag
     2401 tcatgaatgc taagaagcag gttatccctg gcttgtatgg tgctggtgag gttactggcg
     2461 gtgttcatgg tgctaaccgc ttaggtggta atgctatttc agacatcatc accttcggtc
     2521 gcttagcggg tgaagaagct gcaaaatatt ctaaaaagaa ctaatcgatt tagaattcac
     2581 tgactcgcta gtatcagtaa gccacagttc attgctgtgg ctttttttat gcgtcaatat
     2641 ctccatacct atcgaattaa cctcaactag gggtagagat gagaatagaa ttataaagat
     2701 attaagctta gccattaacc actctcaaac ttgtcatttg ttttactaac aaggcgaatt
     2761 aacccgtcaa tagctggcct attgcaagtc ttctcatgta aaaaataacg cagttagcaa
     2821 gacaaagggc tgtttgcgag gcgtttgtta tctcgagttc tggttaatta atgcataatc
     2881 gatggcactg aaaattttgc attaactcat tagctaattt acaacgctca ttcactggct
     2941 aatttacaaa gctatcaaag gataataatg aaacagtcta tagttcacat cgcgctagtg
     3001 gtgaatgatt acgatgaagc catcgatttt tatgttaata aattgaagtt tgatttaatt
     3061 gaagacacct atcaagcaga acaagacaaa cgctgggttg tggtgtcgcc tccggggtca
     3121 aacggagtct cgttgctgct agcaagggca tcaaaaccag agcagcatga ttttattggt
     3181 aaccaagccg gcggacgggt atttcttttt ttgaataccg acgatttttg gcgcgactat
     3241 aaccgcatgc aactcgatgg gattaagttt gtgcgtccgc ctcaagagca agactacggc
     3301 acagtggcag tttttgaaga tct
//