From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human mRNA for 80K-L protein, complete cds.
Date: 5 Nov 92 23:09:59 GMT
Distribution: bionet
Checksum: 22241 6

LOCUS       HUMKCS       2589 bp ss-mRNA            PRI       17-OCT-1992
DEFINITION  Human mRNA for 80K-L protein, complete cds.
ACCESSION   D10522 D90498
KEYWORDS    calmodulin binding protein; cytoplasm; plasma membrane;
            protein kinase C substrate.
SOURCE      Homo sapiens cell line A431 (library: lamda gt10) cDNA to mRNA,
            clones lambda80L-[1,2].
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 2589)
  AUTHORS   Sakai,K.
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
REFERENCE   2  (sites)
  AUTHORS   Sakai,K., Hirai,M., Kudoh,J., Minoshima,S. and Shimizu,N.
  TITLE     Molecular cloning and chromosomal mapping of a cDNA encoding human
            80K-L protein: major substrate for protein kinase C
  JOURNAL   Genomics 14, 175-178 (1992)
  STANDARD  full automatic
COMMENT     Submitted (09-SEP-1991) to DDBJ by: Nobuyoshi Shimizu Department of
            Molecular Biology Keio University School of Medicine 35
            Shinanomachi Shinjuku-ku Tokyo 160 Japan Phone:  03-3351-2370 Fax:
              03-3351-2370

               [2]  sites.
FEATURES             Location/Qualifiers
     polyA_site      2589
     CDS             370..1368
                     /note="tentative"
                     /gene="80K-L"
                     /product="80K-L protein"
                     /codon_start=1
                     /translation="MGAQFSKTAAKGEAAAERPGEAAVASSPSKANGQENGHVKVNGD
                     ASPAAAESGAKEELQANGSAPAADKEEPAAAGSGAASPSSAEKGEPAAAAAPEAGASP
                     VEKEAPAEGEAAEPGSATAAEGEAASAASSTSSPKAEDGATPSPSNETPKKKKKRFSF
                     KKSFKLSGFSFKKNKKEAGEGGEAEAPAAEGGKDEAAGGAAAAAAEAGAASGEQAAAP
                     GEEAAAGEEGAAGGDPQEAKPQEAAVAPEKPPASDETKAAEEPSKVEEKKAEEAGASA
                     AACEAPSAAGPGAPPEQEAAPAEEPAAAAASSACAAPSQEAQPECSPEAPPAEAAE"
BASE COUNT      608 a    659 c    682 g    640 t
ORIGIN      Chromosome 6.
        1 caaccaggga gatttctcca ttttcctctt gtctacagtg cggctacaaa tctgggattt
       61 ttttattact tctttttttt tcgaactaca cttgggctcc tttttttgtg ctcgactttt
      121 ccaccctttt tccctccctc ctgtgctgct gctttttgat ctcttcgact aaaatttttt
      181 tatccggagt gtatttaatc ggttctgttc tgtcctctcc accaccccca cccccctccc
      241 tccggtgtgt gtgccgctgc cgctgttgcc gccgccgctg ctgctgctgc tcgccccgtc
      301 gttacaccaa cccgaggctc tttgtttccc ctcttggatc tgttgagttt ctttgttgaa
      361 gaagccagca tgggtgccca gttctccaag accgcagcga agggagaagc cgccgcggag
      421 aggcctgggg aggcggctgt ggcctcgtcg ccttccaaag cgaacggaca ggagaatggc
      481 cacgtgaagg taaacggcga cgcttcgccc gcggccgccg agtcgggcgc caaggaggag
      541 ctgcaggcca acggcagcgc cccggccgcc gacaaggagg agcccgcggc cgccgggagc
      601 ggggcggcgt cgccctcctc ggccgagaaa ggtgagccgg ccgccgccgc tgcccccgag
      661 gccggggcca gcccggtaga gaaggaggcc cccgcggaag gcgaggctgc cgagcccggc
      721 tcggccacgg ccgcggaggg agaggccgcg tcggccgcct cctcgacttc ttcgcccaag
      781 gccgaggacg gggccacgcc ctcgcccagc aacgagaccc cgaaaaaaaa aaagaagcgc
      841 ttttccttca agaagtcttt caagctgagc ggcttctcct tcaagaagaa caagaaggag
      901 gctggagaag gcggtgaggc tgaggcgccc gctgccgaag gcggcaagga cgaggccgcc
      961 gggggcgcag ctgcggccgc cgccgaggcg ggcgcggcct ccggggagca ggcagcggcg
     1021 ccgggcgagg aggcggcagc gggcgaggag ggggcggcgg gtggcgaccc gcaggaggcc
     1081 aagccccagg aggccgctgt cgcgccagag aagccgcccg ccagcgacga gaccaaggcc
     1141 gccgaggagc ccagcaaggt ggaggagaaa aaggccgagg aggccggggc cagcgccgcc
     1201 gcctgcgagg ccccctccgc cgccgggccc ggcgcgcccc cggagcagga ggcagccccc
     1261 gcggaggagc ccgcggccgc cgcagcctcg tcagcctgcg cagccccctc acaggaggcc
     1321 cagcccgagt gcagtccaga agccccccca gcggaggcgg cagagtaaaa gagcaagctt
     1381 ttgtgagata atcgaagaac ttttctcccc cgtttgtttg ttggagtggt gccaggtact
     1441 gttttggaga acttgtctac aaccagggat tgattttaaa gatgtctttt tttattttac
     1501 ttttttttaa gcaccaaatt ttgttgtttt tttttttctc ccctccccac agatcccatc
     1561 tcaaatcatt ctgttaacca ccattccaac aggtcgagga gagcttaaac accttcttcc
     1621 tctgccttgt ttctctttta ttttttattt tttcgcatca gtattaatgt ttttgcatac
     1681 tttgcatctt tattcaaaag tgtaaacttt ctttgtcaat ctatggacat gcccatatat
     1741 gaaggagatg ggtgggtcaa aaagggatat caaatgaagt gataggggtc acaatgggga
     1801 aattgaagtg gtgcataaca ttgccaaaat agtgtgccac tagaaatggt gtaaaggctg
     1861 tctttttttt tttttttaaa gaaaagttat taccatgtat tttgtgaggc aggtttacaa
     1921 cactacaagt cttgagttaa gaaggaaaga ggaaaaaaga aaaaacacca atacccagat
     1981 ttaaaaaaaa aaaaacgatc atagtcttag gagttcattt aaaccatagg aacttttcac
     2041 ttatctcatg ttagctgtac cagtcagtga ttaagtagaa ctacaagttg tataggcttt
     2101 attgtttatt gctggtttat gaccttaata aagtgtaatt atgtattacc agcagggtgt
     2161 ttttaactgt gactattgta taaaaacaaa tcttgatatc cagaagcaca tgaagtttgc
     2221 aactttccac cctgcccatt tttgtaaaac tgcagtcatc ttggaccttt taaaacacaa
     2281 attttaaact caaccaagct gtgataagtg gaatggttac tgtttatact gtggtatgtt
     2341 tttgattaca gcagataatg ctttcttttc cagtcgtctt tgagaataaa ggaaaaaaaa
     2401 tcttcagatg caatggtttt gtgtagcatc ttgtctatca tgttttgtaa atactggaga
     2461 agctttgacc aatttgactt agagatggaa tgtaactttg cttacaaaaa ttgctattaa
     2521 actcctgctt aaggtgttct aattttctgt gagcacacta aaagcgaaaa ataaatgtga
     2581 ataaaatgt
//