From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: C.glutamicum cop1 gene for PS1
Date: 11 Sep 92 10:03:10 GMT
Distribution: bionet
Checksum: 46580     6

LOCUS       CORCOP1G     2547 bp ds-DNA             BCT       11-SEP-1992
DEFINITION  C.glutamicum cop1 gene for PS1
ACCESSION   X66078
KEYWORDS    cop1 gene; ps1 protein.
SOURCE      Corynebacterium glutamicum DNA.
  ORGANISM  Corynebacterium glutamicum
            Prokaryotae; Firmicutes; Irregular asporogenous rods.
REFERENCE   1  (bases 1 to 2547)
  AUTHORS   Joliff,G., Mathieu,L., Hahn,V., Bayan,N., Duchiron,F., Renaud,M.,
            Shechter,E. and Leblon,G.
  TITLE     Cloning and nucleotide sequence of the csp1 gene encoding PS1, one
            of the two major secreted proteins of Corynebacterium glutamicum:
            The deduced N-terrminal region of PS1 is similar to the
            Mycobacterium antigen 85 complex
  JOURNAL   Mol. Microbiol. 6, 2349-2362 (1992)
  STANDARD  full automatic
COMMENT     *source: strain=ATCC 17965;
            From EMBL    entry CGCOP1G;  dated 02-SEP-1992.
FEATURES             Location/Qualifiers
     prim_transcript <420..2506
     RBS             406..412
     CDS             420..2393
                     /note="one of the twomajor secreted proteins"
                     /product="ps1"
                     /gene="cop1"
                     /codon_start=1
                     /translation="MRDTAFRSIKAKAQAKRRSLWIAAGAVPTAIALTMSLAPMASAQ
                     SSNLSSDAVVGSIAQGVTDGLTDYLKPRVEELPAGEVTYPEIAGLPDGVRVISAEWAT
                     SKHVILTIQSAAMPERPIKVQLLLPRDWYSSPNREFPEIWALDGLRAIEEQSGWTIET
                     NIEQYYADKNAIVVLPIGGESSFYSDWEEPNNGKNYQWETFLTQELAPILDKGFRSNT
                     DRAITGISMGGTAAVNIATHHPDMFKFVGSFSGYLDTTSAGMPIAISAALADAGGYDA
                     NAMWGPVGSERWQENDPKSNVDKLKGKTIYVSSGNGADDFGKEDSVAIGPANATGVGL
                     EVISRMTSQTFVDRANQAGVEVVASFRPSGVHSWEYWQFEMTQAFPHIANALGMSTED
                     RGVECAPVGAIADAVADGAMGTCLTNEYDVTGGKAQDFANGRAYWSANTGAFGLVGRI
                     NARYSELGGPDSWLGYPTSSELKTPDGRGRFVTFEHGSIYWTATTGPWEIPGDMLAAW
                     GTQDYEKGSLGYPTGAAVEYNGGLRQQFEGGYVFRTSNNQSYWVRGEISKKYADDGIF
                     AQLGFPTGNEKLINGGAFQEFEKGNIYWSVSTGAHVILHGDIFDAWGAKGWEQGEYGF
                     PTSDQTAITAGGQTIDFQNGTIRQVNGRIEESR"
     sig_peptide     420..548
     mat_peptide     549..2390
                     /product="ps1"
BASE COUNT      589 a    714 c    708 g    536 t
ORIGIN
        1 aagcttcaag gggaaaacaa gggccttaaa agttatccac agatccgaag tgatccgcgc
       61 actggggtga aaagttatcc acaggaagcg gaggggcgga ttgaaaaatt cagcgaaatg
      121 cgaaaaggtg gaggggaaat gctgcgagtc ttgcggattc ccggcgtggc attgaaaaaa
      181 gtctaaagtt gaacttaaga ttgaggtcat tctgaagttg tgacctgcat cagaagagtt
      241 acatacccac atatgtaacc ttctggacta agatcacgac agactgaaaa gaactgaaga
      301 ctctcaaggc atagcccacg tgtgtttgtc gggccggaag cggggaactt tcgggacgga
      361 tctaactcat tgcgggcctg tgcgcagtat ccaaaaatca aaatgagaag gaaaacttca
      421 tgcgcgacac cgcatttcgt tccatcaagg ctaaagctca ggctaagcgc cgttccctct
      481 ggattgcagc aggcgctgtc ccaaccgcaa ttgcgttgac tatgtccctg gcacctatgg
      541 cttcggctca gtccagcaac ctttcctctg atgccgtagt tggcagcatc gcgcagggcg
      601 tcaccgatgg cctgactgac tacctgaagc ctcgcgtcga agagcttcct gctggtgaag
      661 tcacctaccc agagatcgcc gggctgcctg atggtgtgcg cgtgatcagc gctgagtggg
      721 caacctccaa gcatgtcatt ttgactattc agtctgcagc aatgccagag cgcccaatca
      781 aggtgcagct gctgcttccg cgtgactggt actcttcccc gaaccgtgag ttccctgaaa
      841 tctgggcact tgacggtctg cgcgcgattg aagagcagag tggttggacc attgagacca
      901 acattgagca gtactacgcc gataagaacg ccattgttgt gctcccaatc ggtggcgaga
      961 gctccttcta ctctgactgg gaagagccaa acaacggcaa gaactaccag tgggagacct
     1021 tcctgactca ggagctcgca ccgatcctgg acaagggctt ccgttccaac accgatcgcg
     1081 ccatcaccgg tatctccatg ggcggtaccg ctgcggttaa catcgcaacc caccacccag
     1141 acatgtttaa gttcgtcggt tccttctccg gctatctgga caccacctcc gctggcatgc
     1201 caatcgctat ttccgcagcc ctggcagacg ccggcggata cgatgccaac gcaatgtggg
     1261 gaccagtcgg ttctgagcgc tggcaggaaa acgatccaaa gagcaacgta gacaagctca
     1321 agggcaagac catctacgtt tcctctggta acggtgcaga tgacttcggt aaggaagact
     1381 ctgtagctat tggacctgca aacgcgacag gtgtcggtct ggaagttatc tcccgtatga
     1441 cttcccagac cttcgtcgat cgtgcaaacc aggctggcgt ggaagttgtt gctagcttcc
     1501 gtccatccgg cgtgcactca tgggaatact ggcagttcga gatgactcag gcgttccctc
     1561 acatcgctaa cgctcttggc atgtccactg aggaccgtgg cgttgagtgt gcacctgtcg
     1621 gcgcaatcgc tgacgctgtt gccgacggcg cgatgggcac ctgcctgacc aacgaatacg
     1681 atgttaccgg cggtaaggcc caggacttcg ctaacggtcg cgcatactgg tctgcaaaca
     1741 ctggcgcttt cggcctggtt ggacgcatca acgctcgtta ctctgagctg ggtggacctg
     1801 actcctggtt gggctaccca acctcttctg agttgaagac accagacgga cgtggccgct
     1861 tcgtcacctt cgagcacggc tccatctact ggaccgccac cactggtcct tgggaaatcc
     1921 caggcgatat gctcgccgca tggggcaccc aggactatga gaagggcagc ctcggctacc
     1981 caaccggcgc cgcagttgaa tacaacggtg gcctgcgcca gcagttcgaa ggtggctacg
     2041 tattccgtac ctccaataac cagtcttact gggttcgcgg agaaatctcc aagaagtacg
     2101 ccgatgacgg aatcttcgct cagcttggtt tcccaaccgg caatgagaag ttgatcaacg
     2161 gtggcgcttt ccaggaattc gaaaagggca acatctactg gtccgtgtcc actggcgcgc
     2221 acgtgattct gcacggcgac atcttcgacg catggggtgc taagggctgg gagcagggcg
     2281 aatacggctt cccaacctct gaccagaccg caatcaccgc gggtggacag accattgatt
     2341 tccagaacgg caccatccgt caggtcaatg gccgaattga ggagtctcgc taatagtgaa
     2401 gcgcatctac gcaactctcg cttccggact tttgtgcctg agccttgctg cttgtggggg
     2461 agtcactgtt gaaggagatg attctccctc gacagcggca gccccaacag aaagcagcgc
     2521 tgggtcaagc agcaccgcaa ggtcgac
//