From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Neisseria meningitidis class 1 porin (porA) gene, complete cds.
Date: 29 Sep 92 10:08:04 GMT
Distribution: bionet
Checksum: 15234     5

LOCUS       NGOPORA3S    1151 bp ds-DNA             BCT       29-SEP-1992
DEFINITION  Neisseria meningitidis class 1 porin (porA) gene, complete cds.
ACCESSION   L02929
KEYWORDS    class 1 outer membrane protein; porA gene; porin.
SOURCE      Neisseria meningitidis (individual_isolate case 8529, strain
            B:15:P1.3) DNA.
  ORGANISM  Neisseria meningitidis
            Prokaryotae; Gracilicutes; Scotobacteria; Neisseriaceae.
REFERENCE   1  (bases 1 to 1151)
  AUTHORS   Saunders,N.B., Zollinger,W.D. and Rao,V.B.
  TITLE     Detection of low copy number Meningococci in cerebrospinal fluid by
            nested PCR using PorA as target gene
  JOURNAL   Unpublished (1993)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     CDS             <1..1151
                     /product="porin"
                     /gene="porA"
                     /codon_start=3
                     /translation="LSALPLAAVADVSLYGEIKAGVEGRNYQLQLTEAQAANGGASGQ
                     VKVTKAKSRIRTKISDFGSFIGFKGSEDLGDGLKAVWQLEQDVSVAGGGATQWGNRES
                     FIGLAGEFGTLRAGRVANQFDDASQAIDPWDSNNDVASQLGIFKRHDDMPVSVRYDSP
                     EFSGFSGSVQFVPAQNSKSAYTPATLANGANNTIIRVPAVVGKPGSDVYYAGLNYKNG
                     GFAGNYAFKYARHANVGRDAFNLFLLGRIGDDDEAKGTDPLKNHQVHRLTGGYEEGGL
                     NLALAAQLDLSENGDKTKNSTTEIAATASYRFGNAVPRISYAHGFDFIERGKKGENTS
                     YDQIIAGVDYDFSKRTSAIVSGAWLKRNTGIGNYTQINAASIGLRHKF"
     sig_peptide     1..32
                     /gene="porA"
                     /note="putative"
                     /codon_start=3
                     /partial
     mat_peptide     33..1148
                     /standard_name="class 1 porin"
                     /product="porin"
                     /gene="porA"
                     /note="putative"
                     /label=P1_3
                     /codon_start=1
     misc_feature    102..147
                     /function="exposed immunogenic region"
                     /phenotype="masked subtype 7"
                     /standard_name="variable region 1"
                     /note="subtype identified by monoclonal antibody
                     reactivity topeptide"
                     /gene="porA"
                     /evidence=EXPERIMENTAL
     misc_feature    555..590
                     /function="exposed immunogenic loop"
                     /phenotype="subtype 3"
                     /standard_name="variable region 2"
                     /note="subtype 3 specificity identified by monoclonal
                     antibodyreactivity to peptide"
                     /evidence=EXPERIMENTAL
BASE COUNT      275 a    295 c    311 g    270 t
ORIGIN
        1 tattgtccgc actgccgctt gcggccgttg ccgatgtcag cctatacggc gaaatcaaag
       61 ccggcgtgga aggcaggaac taccagctgc aattgactga agcacaagcc gctaacggtg
      121 gagcgagcgg tcaggtaaaa gttactaagg ccaaaagccg catcaggacg aaaatcagtg
      181 atttcggctc gtttatcggc tttaagggga gtgaggattt gggcgacggg ctgaaggctg
      241 tttggcagct tgagcaagac gtatccgttg ccggcggcgg cgcgacccag tggggcaaca
      301 gggaatcctt tatcggcttg gcaggcgaat tcggtacgct gcgcgccggt cgcgttgcga
      361 atcagtttga cgatgccagc caagccattg atccttggga cagcaataat gatgtggctt
      421 cgcaattggg tattttcaaa cggcacgacg atatgccggt ttccgtacgc tacgattccc
      481 ccgaattttc cggtttcagc ggcagcgtcc aattcgttcc ggcccaaaac agcaagtccg
      541 cctatacgcc ggctactctt gctaatggtg ctaataatac aattattcgc gttccggctg
      601 ttgtcggcaa gcccggatcg gatgtgtatt atgccggtct gaattacaaa aatggcggtt
      661 ttgccgggaa ctatgccttt aaatatgcga gacacgccaa tgtgggccgt gatgctttta
      721 atttgttctt gcttgggcgc atcggcgatg atgatgaagc caaaggtacc gatcccttga
      781 aaaaccatca ggtacaccgc ctgacgggcg gctatgagga aggcggcttg aatctcgcct
      841 tggcggctca gttggatttg tctgaaaatg gcgacaaaac caaaaacagt acgaccgaaa
      901 ttgccgccac tgcttcctac cgcttcggta atgcagttcc gcgcatcagc tatgcccatg
      961 gtttcgactt tatcgaacgc ggtaaaaaag gcgaaaatac cagctacgat caaatcatcg
     1021 ccggcgttga ttatgatttt tccaaacgca cttccgccat cgtgtctggc gcttggctga
     1081 aacgcaatac cggcatcggc aactacactc aaattaatgc cgcctccatc ggtttgcgcc
     1141 acaaattcta a
//