From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: D.pseudoobscura Adh gene for alcohol dehydrogenase
Date: 28 Dec 92 21:27:19 GMT
Distribution: bionet
Checksum: 19336 8

LOCUS       DROADHGN45   3478 bp ds-DNA             INV       01-DEC-1992
DEFINITION  D.pseudoobscura Adh gene for alcohol dehydrogenase
ACCESSION   X62225
KEYWORDS    ADH gene; alcohol dehydrogenase.
SOURCE      Drosophila pseudoobscura DNA.
  ORGANISM  Drosophila pseudoobscura
            Eukaryota; Animalia; Metazoa; Arthropoda; Uniramia; Insecta;
            Pterygota; Neoptera; Holometabola; Diptera; Brachycera;
            Cyclorrhapha; Schizophora; Drosophiloidea; Drosophilidae.
REFERENCE   1  (bases 1 to 3478)
  AUTHORS   Schaeffer,S.W. and Miller,E.L.
  TITLE     Estimates of gene flow in Drosophila pseudoobscura determined from
            nucleotide sequence analysis of the alcohol dehydrogenase region
  JOURNAL   Genetics 132, 471-480 (1992)
  STANDARD  full automatic
COMMENT     *source: strain=MXA31; *source: isolate=Amecameca, Mexico; *source:
            chromosome=IV section 88;
               From EMBL    entry DPADHGN45;  dated 16-OCT-1992.
FEATURES             Location/Qualifiers
     mRNA            join(40..119,915..1020,1084..1488,1551..1966)
                     /note="adult mRNA (Adh)"
     mRNA            join(882..1020,1084..1488,1551..1966)
                     /note="larval mRNA (Adh)"
     promoter        8..14
                     /note="adult promoter (Adh)"
     prim_transcript 40..1966
                     /note="adult primary transcript (Adh)"
     exon            40..119
                     /note="adult exon 1a"
     intron          120..914
                     /note="adult intron"
     promoter        846..853
                     /note="larval promoter (Adh)"
     prim_transcript 882..1966
                     /note="larval primary transcript (Adh)"
     exon            882..1020
                     /note="larval exon 1"
     exon            915..1020
                     /note="adult exon 1b"
     intron          1021..1083
                     /number=1
     exon            1084..1488
                     /number=2
     intron          1489..1550
                     /number=2
     exon            1551..1966
                     /number=3
     polyA_signal    1943..1948
     polyA_site      1966
     CDS             join(928..1020,1084..1488,1551..1817)
                     /EC_number="1.1.1.1"
                     /gene="Adh"
                     /product="alcoholdehydrogenase"
                     /codon_start=1
                     /translation="MSLTNKNVVFVAGLGGIGLDTSRELVKRNLKNLVILDRIDNPAA
                     IAELKAINPKVTITFYPYDVTVPVAETTKLLKTIFAQVKTIDVLINGAGILDDHQIER
                     TIAVNYTGLVNTTTAILDFWDKRKGGPGGIICNIGSVTGFNAIYQVPVYSGSKAAVVN
                     FTSSLAKLAPITGVTAYTVNPGITKTTLVHKFNSWLDVEPRVAEKLLEHPTQTSQQCA
                     ENFVKAIELNKNGAIWKLDLGTLEPITWTQHWDSGI"
BASE COUNT      980 a    795 c    794 g    909 t
ORIGIN      
        1 cgtagtgtat ttaaggcact gcacatgtcg aggaccacga ttattgtcta cagtgcagtc
       61 agtagttgtc aggtgcagca ggcggaccag catttcttta aatctactca atcgatcaag
      121 taagtaactg taatccgagt acagtcggcc catcccactc ggttctgcag taactcaaga
      181 aatttcttga tagcgcagtt ccagttgtaa aacgtcgttt aaaaaaacat ctcgttggag
      241 cttgggctga aaaacattcg agttgggcgt agaaaatttc aaatataaaa tatcaaaaaa
      301 gagaaaatat cttcccaaag cagctgtcca ttttttgtta aagtgataga tcaaaaattc
      361 gtatgacgta tttcccaagt gctcttcatg caaataatgt cctcaattct aattctacaa
      421 gaagcacttt tggctcttgt ccagaaaaaa caatttgttt acacttttcg gttgataaca
      481 gaagcggacc gacggctaag taacataaca gtcgcctgtt aagtaatgcc ctcactctct
      541 gttagatggc cgcgcgtgtc gactccgata gaccagcaga cacgcagtag ttttcctcaa
      601 gaacctgaac tctaaacata gacataattt acgatacctt gagactcaaa taaaaataca
      661 agtatgagtg gtattctatt cctgattaga ctcgcacaca cacacgtacg agtattaaca
      721 taaatttgcc ggcaaataac caaaacaaaa cgaacgcaaa cgatccgaac acgtaatgcg
      781 agagataaga aacagaaaag ctcgacgtga aagaagcttc tggcggaagc tcttgccgaa
      841 ctccctataa atacgagaca gaaaaccaga ataaatataa taacaaactg ttgaattgtc
      901 ccatcgattc ccagatcaaa agacagaatg tctctcacca acaagaacgt cgttttcgtg
      961 gccggtctgg gcggcattgg cctggacacc agccgggagt tggtcaagcg taatctgaag
     1021 gtaagagaga acgagttcca tggaatctat ggaatcctaa atttaaaaat tcatttattt
     1081 tagaacctgg tcatcctgga tcgcattgac aatccggctg ccattgccga actgaaggca
     1141 atcaatccca aggtgaccat caccttctat ccctacgatg tgactgtgcc cgtcgctgag
     1201 accaccaagc tcctgaagac catctttgcc caggtgaaga caatcgatgt cctgatcaac
     1261 ggtgctggca tcctggacga tcatcagatc gagcgcacca ttgccgttaa ctacacgggc
     1321 ctggtcaaca ccaccacagc cattctggac ttctgggaca agcgcaaggg cggcccaggc
     1381 ggcatcattt gcaacattgg ctccgtcacc ggtttcaatg ccatctacca ggtgcccgtt
     1441 tactccggct ccaaggcggc ggtggttaac tttacctcct ccctggcggt aagaaatcct
     1501 acaactatcc caaaatcccc aaaactaaat atttctaaat attcccatag aaactggctc
     1561 ccattactgg tgtcactgct tacactgtca atcctggcat cactaagacc actctggtcc
     1621 acaaattcaa ctcgtggctg gatgtggagc cccgtgtggc ggagaagctg ctcgagcatc
     1681 ccacccagac ctctcagcag tgcgccgaga actttgtgaa ggccattgag ctgaacaaga
     1741 acggtgccat ctggaagttg gatttgggca ccttggagcc catcacatgg acccagcact
     1801 gggactcggg catctaaacc agcccagaga ctctatggga catggcgttt tagcttttag
     1861 tttctttttt tccactcaat tgttacgtat atacatacac atatggcaat aaggctgatt
     1921 tgaaccccgt tttgaatatg ataatacaaa attatatttg aaaaatttca acaaaatcga
     1981 taagagctct agctctcttt taattaaaaa aaactagata aaaagagcaa tggtcagtgg
     2041 tggtcagtgg tcgttgtggc ctctatcgat ttccacacaa aaactttact gtttagtaat
     2101 agaaaagaac ttgagaaagg cagccaaaag aatgtacgat ctgacgggta agcatgtctg
     2161 ctatgtggct gactgcggtg gcattgcact ggagaccagc aaggttctca tgaccaagaa
     2221 tatagcggtg agtggtaaag tggtgtagag agtggagagt gttccgaagt ggttctggtt
     2281 cttaaacaag tctctagtct ctagtctctt ggctgctgct ggtagctgtc gaaactaatt
     2341 aagttaatga ctttttcatg ttttgttttt gtccgctgac ttttgctgtt caaagctaca
     2401 attaagttta tgttttgagt ttgatctact tttgcttaag ggaagtactt tcgattacgg
     2461 attgctggca tataaaaata gataccaagg aatgtgcatc atatcatatt gattctatct
     2521 tgattctttg cctagaaact ggccatcctt cagagcgtgg aaaacccccc ggccatcgct
     2581 cagttacaat cgattaagca cagcacacag atcttcttct ggaccttcga tgtgaccatg
     2641 gcccgcgagg agatgaagaa gtacttcgat gaggtcatgg tccagatgga ctacatcgat
     2701 gtactcatca atggggccac cctgtgcgat gagcggaaca ttgatgccac catcaatacg
     2761 aatctgaccg gaatgatgaa caccgtggcc actgtgctgc cctatatgga ccgaaagatg
     2821 ggcggttcgg gtggactgat cgtgaatgtc acctccgtca taggactgga tccatcgccg
     2881 gtcttttgtg cgtacagtgc ctcaaagttt ggtgtgattg ggttcacccg aagtctagcg
     2941 gtgagtgggc gagatcttag cttgaaaatg ctctctaatg tcttccattc tttcgcagga
     3001 tcccctgtat tacacccaaa atggtgtggc tgtcatggcc gtctgctgtg gtcccaccaa
     3061 agtgtttgtc gatcgggaac tgactgcctt cctggaatac ggccagtcct ttgccgatcg
     3121 cctgcgtcgt gccccctgcc aatcgacggc cgtctgcggc cagaatatag taaatgccat
     3181 cgagagatcg gagaacggac agatctggat tgccgacaag ggcggcctgg agtcggtggc
     3241 cctccactgg tattggcata tggccgatca gtttgtgaac tacatgcaga gcactgacga
     3301 tgaggatcag gagttttttt tgggccagcg atgaggcatg ccttgatcca cggaataaaa
     3361 catcaaatgt gtcacaaaaa gtaggcaact tgtttgtggg tttggaaata gatttgaaat
     3421 attttgaggt catatgaata atgacataaa tgagggtgcc tgaaagtatg ccaagttt
//