From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: N.tabacum PR-4B mRNA for pathogenesis-related protein 4B
Date: 10 Apr 92 10:13:59 GMT
Distribution: bionet
Checksum: 56931     3

LOCUS       TOBPR4B       599 bp ss-mRNA            PLN       10-APR-1992
DEFINITION  N.tabacum PR-4B mRNA for pathogenesis-related protein 4B
ACCESSION   X58547
KEYWORDS    PR-4B gene; pathogenesis-related protein.
SOURCE      Nicotiana tabacum RNA.
  ORGANISM  Nicotiana tabacum
            Eukaryota; Plantae; Embryobionta; Magnoliophyta; Magnoliopsida;
            Asteridae; Solanales; Solanaceae.
REFERENCE   1  (bases 1 to 599)
  AUTHORS   Linthorst,H.J., Dankash,N., Brederode,J.T., van Kan,J.A.,
            De Wit,P.J. and Bol,J.F.
  TITLE     Tobacco and tomato PR proteins homologous to win and pro-hevein
            lack the
  JOURNAL   Mol. Plant Microb. Interact. 4, 586-592 (1991)
  STANDARD  full automatic
COMMENT     *source: strain=Samsun NN; *source: stage=8 weeks old plant,
            infected with tobacco mosaic *source: virus; *source: tissue=leaf;
            *source: clone_library=lambda ZAP cDNA; *source: clone=CPR4-5,
            CPR4-2, CPR4-8;
            mRNA is induced in tobacco plants, hypersensitively reacting to
            infection with tobacco mosaic virus and upon certain other
            stresses.
            From EMBL    entry NTPR4B;  dated 19-MAR-1992.
FEATURES             Location/Qualifiers
     CDS             <1..421
                     /product="pathogenesis-related protein 4B"
                     /gene="PR-4B"
                     /evidence=EXPERIMENTAL
                     /codon_start=2
                     /translation="LCVALLIMSVMMAMAAAQSATNVRSTYHLYNPQNINWDLRAASA
                     FCATWDADKPLAWRQKYGWTAFCGPAGPRGQDSCGRCLRVTNTGTGTQATVRIVDQCS
                     NGGLDLDVNVFNQLDTNGLGYQQGHLIVNYEFVNCND"
     sig_peptide     <1..52
                     /product="pathogenesis-related protein 4B"
                     /gene="PR-4B"
                     /codon_start=2
     mRNA            1..599
                     /note="partial cDNA, clone CPR4-5"
                     /gene="PR-4B"
     mat_peptide     53..418
                     /product="pathogenesis-related protein 4B"
                     /gene="PR-4B"
     polyA_signal    576..581
                     /evidence=EXPERIMENTAL
     polyA_site      599
                     /evidence=EXPERIMENTAL
BASE COUNT      166 a    112 c    155 g    166 t
ORIGIN
        1 gttgtgcgtg gcattgctga tcatgagcgt gatgatggca atggcggcgg cacagagcgc
       61 tacaaacgtg agatcaacgt atcatttata taacccacag aacattaact gggatttgag
      121 agcagcaagt gctttctgcg ctacttggga tgccgataag cctctcgcat ggcgccaaaa
      181 atatggctgg actgctttct gcggtcctgc tggacctcga ggccaagatt cctgtggtag
      241 atgcttgagg gtgacgaaca ccggaacagg aactcaagca acagtgagaa tagtagatca
      301 atgcagcaat ggagggcttg atttggatgt gaatgtcttt aaccaattgg acactaatgg
      361 attgggctat cagcaaggcc accttattgt caactatgaa tttgtcaact gcaatgacta
      421 attaatctgc ttccagaaat taagtagcta ctgtagtatc ttatttttca gacgtgcgct
      481 gttagaattg agaagggggc tgagatgctt ttaatagtat actgtagtaa ttatctgaaa
      541 gctctacatg tttggttgcc cttcacgatt aatagaataa aagcttgggc ttagtaggg
//