From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: S.vulgare NADH II gene for NADP-malate dehydrogenase
Date: 24 Jul 92 10:10:09 GMT
Distribution: bionet
Checksum: 03479    10

LOCUS       SRGCPNADPM   4759 bp ds-DNA             ORG       24-JUL-1992
DEFINITION  S.vulgare NADH II gene for NADP-malate dehydrogenase
ACCESSION   X54404
KEYWORDS    NADH II gene; NADP-malate dehydrogenase.
SOURCE      Chloroplast Sorghum vulgare DNA.
  ORGANISM  Chloroplast Sorghum vulgare
            Eukaryota; Plantae; Embryobionta; Magnoliophyta; Liliopsida;
            Commelinidae; Cyperales; Poaceae.
REFERENCE   1  (bases 1 to 4759)
  AUTHORS   Luchetta,P., Cretin,C. and Gadal,P.
  TITLE     Organization and expression of the two homologous genes encoding
            the NADP malate dehydrogenase in Sorghum vulgare leaves
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
COMMENT     *source: strain=cv INRA 450; *source: stage=6 days; *source:
            tissue=leaf; *source: clone_library=lambda EMBL4 genomic; *source:
            clone=NMDHII;
            From EMBL    entry SVNADPMG;  dated 16-APR-1992.
FEATURES             Location/Qualifiers
     promoter        <1..1082
     CDS             join(1111..1250,1387..1483,1909..1995,2093..2177,
                     2344..2525,2816..2886,2964..3054,3144..3218,3308..3409,
                     3512..3634,3719..3787,3861..3929,4022..4075,4179..4232)
                     /EC_number="1.1.1.82"
                     /product="malate dehydrogenase (NADP+)"
                     /gene="NADH II"
                     /codon_start=1
                     /translation="MGLSTAYSPAGSGLVPAPLARAARRRSVQVRRPRLATVRCSVVD
                     AAKQVQDGVATAVGGGAASGNECFGVFCNIYDLKAEDKTKSWKKLVTIAVSGAAGMIS
                     NHLLFKLASGEVFGQDQPIALKLLGSERSFQALEGVRMELEDSLYPLLREVSIGIGPY
                     EVFQDVDWALLIGAKPRGPGMERAALLDINGQIFADQGKALNAVASWNVKVLVVGNPC
                     NTNALICLKNTPNIPAKNFHALTRLDENRAKCQIALKAGVFYDKVSNVTIWGNHSTTQ
                     VPDFLNAKIDGRPVKEIIQDTKWLEEEFTMTVQKRGGVLIQKWGRSSAASTAVSIVDA
                     IKSLVTPTPEGEWFSTGVYTTGNPYGIAEDIVFSMPCRSKGDGDYELATDVSMDDFLW
                     EWIKKSEAELLAEKKCVAHLTGEGDAFCDLPEDTMLPGEN"
     TATA_signal     1004..1011
     prim_transcript 1083..4759
     exon            1111..1250
                     /number=1
     intron          1251..1386
                     /number=1
     exon            1387..1483
                     /number=2
     intron          1484..1908
                     /number=2
     exon            1909..1995
                     /number=3
     intron          1996..2092
                     /number=3
     exon            2093..2177
                     /number=4
     intron          2178..2343
                     /number=4
     exon            2344..2525
                     /number=5
     intron          2526..2815
                     /number=5
     exon            2816..2886
                     /number=6
     intron          2887..2963
                     /number=6
     exon            2964..3054
                     /number=7
     intron          3055..3143
                     /number=7
     exon            3144..3218
                     /number=8
     intron          3219..3307
                     /number=8
     exon            3308..3409
                     /number=9
     intron          3410..3511
                     /number=9
     exon            3512..3634
                     /number=10
     intron          3635..3718
                     /number=10
     exon            3719..3787
                     /number=11
     intron          3788..3860
                     /number=11
     exon            3861..3929
                     /number=12
     intron          3930..4021
                     /number=12
     exon            4022..4075
                     /number=13
     intron          4076..4178
                     /number=13
     exon            4179..4232
                     /number=14
     terminator      4233..4759
BASE COUNT     1308 a   1063 c   1044 g   1344 t
ORIGIN
        1 aggccttgtt tacttacact ctaggaccca acattttcca agatgtctta tcacatcgaa
       61 tctttagata aatatatgga gtattaaata tagacgaaaa aaactaattg cacaatttga
      121 tcggaattga tgagacgaat cttttgaggc tagttagtct atgattggac aataattact
      181 acaaacaaac gaaagtacta tagtgtcgtg aaaattttat cttcacgaac taaacgtgcg
      241 gcctaaacga acagtacgtg ggttcttggt aacgatgaga agttgtacca tcacactctt
      301 ttgaaaagac gaggatgccg actttgtgac tcaatacact agcgaagatt gttgtgggag
      361 aatataacct ttttttttgg ttgccttcat tttggtgtgc ctttctctgg gaaccaaatc
      421 tgtcaatgag tctttttaaa aataaaaaaa atgctacctg taaaaactgt tattctccag
      481 ttctttttaa aatgattttt ttttctctga aactcctttt taaaatgact ttgtacttgt
      541 cactgttctc ctcagactga ggaaccgcca ggatagcaga cgtgtggacg tacaagctgc
      601 ccaacaaccg ccgtcctgcc acgtgtcacg cggcgggcgg tccgcttgcc tttgccgcca
      661 aatccaatcc aattccttcg cgcgctactg cgtagtatgt tacacggata ctctaaagaa
      721 ggtgacgtat ccgtatccga tactcgtcgg atacgcgaat acggatactc ggatacacta
      781 ttttagtagt tttttttttg agaaagtgca tatccgcgta tccaatacgt aaatccaatc
      841 caattccttc gcgcgctact gcgtagtatt tacacggata ctcaaagaag gtgacgtatc
      901 cgtatccgat actcgtcgga tacgcagata cggatactcg gatacactat tttagtaggt
      961 tttcttttga gaaaatgcat atccgcgtat ccaatacgta ttgtatcgaa tactcgttag
     1021 ctgcgtagtg caagcaagtg caactcctcc tatccatccg gcacgcccag catccatcca
     1081 ctcctctgct ccacctccag ctcctcctcc atgggccttt caacagctta ctccccggcc
     1141 gggtcgggcc ttgtgccggc ccctctcgcg cgcgccgcgc gccgtcgctc cgtccaggtc
     1201 cgccgcccgc gcctcgccac cgtccgatgc tccgtcgtcg acgccgccaa gtgagtagta
     1261 gtacataccg tgtttcagct cacgcctagc tataaccccc gatgtgtgtg tgtggacaca
     1321 tgaatgaatg cgtgccagca gcttgatcgg ctgactagcg tgtacgtctg atgagaaccc
     1381 cagcaggcag gtgcaggacg gcgtggcgac ggcggtcggc ggaggcgcgg cgtccgggaa
     1441 cgagtgcttc ggggtcttct gcaacatcta cgacctcaag gcggtgagtc cgtcagctca
     1501 catcacatgg tcctccgttc ccccagtcag tccgtccggc ctccccgtgt tcgtgaatgc
     1561 tccatcggct tggcttcttc tgctgcctct ctggtttgct ctacgttcgc cactagctac
     1621 ggtactggat agcgagcaca catataaact tcgtacagat caccaaacga aagcgcaggg
     1681 tggaatgcca gcgtcattga tagctgtgcg cttgctgcac gcacctctag ctgcgtgatc
     1741 aagcgctttc attgccagac agcaagtgag caaagatgtt aacgtctaac gcctcctgaa
     1801 ttgcatgcat ttcttgactt aatttggttt ttatgctgct tgtatcatca cataatacag
     1861 tactaacaac ctgtcatcct acgccgttga atggattggg acacacagga agacaagacc
     1921 aagtcgtgga agaagctagt gaccattgct gtgtcaggcg cggctgggat gatttcaaat
     1981 cacctgctgt tcaaagtaaa caaatctttt actgactctg catattttct acctaccgag
     2041 aggcagtatt gtattgttga caattcggtt tattaaccca aaaaaaattc agctcgcctc
     2101 cggtgaggtt tttggacaag accaaccaat agcacttaag ttacttggct cagaaagatc
     2161 gtttcaagct ctagaaggtg aaagatttct tctttcggcc ttagtactat ttgagaagaa
     2221 gagtcaattc atatttgcta gtacatagtt taagctccaa actaccccta caatcaatgt
     2281 attactttga cagtgaatat gtatcacttg ttcctcatgt gctctcaaaa actgcaactg
     2341 caggcgtacg tatggaactg gaggactcgc tgtatccact gctgagggaa gtcagcattg
     2401 gtataggtcc ttacgaggtc tttcaagatg tagattgggc ccttcttatt ggtgctaagc
     2461 cccgaggtcc cggcatggag cgagctgcat tactggatat caatggccaa atctttgctg
     2521 accaggtttc tttccactct gttcgtcgtt tcttctaaaa tactatgaca tgaaattgta
     2581 aaaaaccctc tcaggaaggg tgccaatagg catttctgaa acttcagtat ttgaccctca
     2641 atttaagaag gttccttagc atagaacctg caaactgctg aaaccagaag cctcgtataa
     2701 atttcagtac agcgcgcata aaccagaaac caagtataaa tttgttcaca gtgtgcaaaa
     2761 aacaacagag taactgtatt tttgttatgg atctgaaatt ttgaattgtg ttcaggggaa
     2821 agcacttaat gccgtggcct cgcggaacgt gaaagtctta gttgttggaa atccctgtaa
     2881 cactaagtac ggatatttat atgttttgct gcttactaac gatttatgcc atcgacttag
     2941 ttttgggttg acacttgatg cagtgcattg atttgcttga aaaatactcc aaacatacca
     3001 gcaaaaaatt ttcatgcact gacgaggttg gacgaaaata gagcaaagtg ccaggtgact
     3061 gacgttgaac ctttattatg tctcgtcttt ccattcccaa caggaaaaca gatcaagcaa
     3121 tcacacacaa tctccttttg tagatagcac tcaaagcagg tgtattttat gacaaagtat
     3181 caaacgtgac tatttggggg aaccattcga caactcaggt gaagattaga ttattataaa
     3241 ctagatgagc agactatttt tgggatacat ttcttccctg attgttggca ctaacttccc
     3301 tgtgtaggtt cctgatttct tgaatgccaa aattgatggg agaccagtga aagaaatcat
     3361 tcaggatacc aagtggttag aagaagagtt caccatgaca gttcaaaagg tgtgtgcctc
     3421 tccctgtttg cagtacgttt ggaggtttct gttactcgag aaattaagtc aaggttgtac
     3481 cataattcgt ttcattccta tatctaaaca gcgtggaggt gtgctcatcc aaaaatgggg
     3541 cagatcttca gccgcatcaa cggctgtttc gatagtggat gctattaaat cccttgtaac
     3601 tcctacccca gaaggcgagt ggttctctac aggggtaagt tttgaatcct ttgaattgaa
     3661 attggtcaca gctttgtttc agcattcctc taatggaaga agcaataaaa aaacacaggt
     3721 ttatacgact ggaaatcctt atggcatagc agaggatatc gtgttcagca tgccatgcag
     3781 atcgaaggta cgatcttact aatcaaactg tcctcattat tttcgttacc actctgatga
     3841 tcataaaatt cttccaacag ggtgatggtg attacgaact agctactgat gtgtcaatgg
     3901 acgattttct gtgggaacgg attaaaaagg taggggctat aggaactgcc atcactgata
     3961 tgctgagaat ataagcagca tagagatttt accctaacag ttgaccctgc aatattttca
     4021 gagtgaagct gaattgcttg ctgagaagaa atgcgttgcc catcttactg gagaggtatg
     4081 ccacagtggc gtattttagc ttaacgatgt gtgcgtgtaa aaagagaaat agtgccggaa
     4141 agtattggct tagctttttt tttgtttgtg caatgcaggg ggatgcattt tgtgatcttc
     4201 cggaagatac catgctacca ggagaaaatt aatttagtta cagaagcaat agcagtggaa
     4261 tgcatttgtt tatgttagta cagttcctgg cgaaaacaca gaaactttga gagagacagc
     4321 tgcttgcaag atgcagcaac tacttatata tacatatata atataagctc tatcaactaa
     4381 aatcatacga agtactggcc aacagtgcac cacacatgaa tgacctagta tctacagctc
     4441 agaacgccct cattaagagg agatacatcc acgataactt catttacgcg agttgcataa
     4501 gaagccaact cttttataaa gttggacatc tgaagtgttt gactctattg gttggagctt
     4561 cctattagag gtacttgaga acttggggtt cagtacaaaa tggagagatt ggaacgcggt
     4621 gttattaggc acatcaactc aagagtgtct tgatcaaggg acaaccgacg aaggagattc
     4681 gacatgaacc gtgggctcag gcagggagac ccccctctcc caatctacct tttcatcttg
     4741 gccatccgac ccactgcag
//