From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Rat branched chain alpha-keto acid dehydrogenase (BCKDHB)
Date: 31 Dec 92 01:39:30 GMT
Distribution: bionet
Checksum: 57167 4

LOCUS       RATAKAD      1384 bp ss-mRNA            ROD       17-DEC-1992
DEFINITION  Rat branched chain alpha-keto acid dehydrogenase (BCKDHB)
            E1-subunit mRNA, 3'end.
ACCESSION   M94040
KEYWORDS    branched chain alpha-keto acid dehydrogenase E1-beta subunit.
SOURCE      Rattus norvegicus (strain Sprague-Dawley) adult liver cDNA to mRNA.
  ORGANISM  Rattus norvegicus
            Eukaryota; Animalia; Chorqata; Vertebrata; Mammalia; Theria;
            Eutheria; Rodentia; Myomorpha; Muridae; Murinae.
REFERENCE   1  (bases 1 to 1384)
  AUTHORS   Zhao,Y., Kuntz,M.J., Harris,R.A. and Crabb,D.W.
  TITLE     Molecular cloning of the E1-beta subunit of the rat branched chain
            alpha-ketoacid dehydrogenase
  JOURNAL   Biochim. Biophys. Acta 1132, 207-210 (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     sig_peptide     <1..83
                     /gene="BCKDHB"
                     /codon_start=1
     mat_peptide     84..1109
                     /function="decarboxylation of branched-chain alpha-keto
                     acid"
                     /product="branched chain alpha-keto acid dehydrogenase
                     E1-beta subunit"
                     /gene="BCKDHB"
                     /codon_start=1
     3'UTR           1110..1384
     CDS             <1..1112
                     /gene="BCKDHB"
                     /product="branched chain alpha-keto acid dehydrogenase
                     E1-beta subunit"
                     /codon_start=3
                     /translation="CNSADWPAALVQGFLQPAVDDASQKRRVAHFTFQPDPESLQYGQ
                     TQKMNLFQSITSALDNSLAKDPTAVIFGEDVAFGGASDVLLAYETNMEKTECLTPPLC
                     EQGIVGFGIGIAVTGATAIAEIQFADYIFPAFDQIVNEAAKYRYRSGDLFNCGSLTIR
                     APWGCVGHGALYHSQSPEAFFAHCPGIKVVIPRSPFQAKGLLLSCIEDKNPCIFFEPK
                     ILYRAAVEQVPVEPYRIPLSQAEVIQEGSDVTLVAWGTQVHVIREVASMAQEKLGVSC
                     EVIDLTTIVPWDVDTVCKSVIKTGRLLISHEAPLTGGFASEISSTVQEECFLNLEAPI
                     SRVCGYDTPFPHIFEPFYIPDKWKCYDALRKMINY"
BASE COUNT      333 a    324 c    348 g    379 t
ORIGIN      
        1 actgcaattc ggcggattgg cctgctgcgc tggtgcaagg cttcttgcaa cccgctgtcg
       61 atgacgcgtc ccagaagcgg cgggtggccc acttcacttt ccagccggac ccagagtccc
      121 ttcagtatgg gcaaactcag aaaatgaacc tcttccagtc aataacaagt gccttggata
      181 actcattggc caaagaccct actgcagtaa tatttggtga ggacgttgcc tttggtggag
      241 cttccgatgt actgttggct tacgagacaa atatggaaaa gacagagtgt ttaacacccc
      301 cgttgtgtga acaaggaatt gttgggtttg gcattggaat cgcggtcact ggtgctacag
      361 ctattgcgga aatccagttt gccgactata ttttccctgc ctttgatcag attgtcaacg
      421 aagctgccaa gtatcgatac cgctctggcg atcttttcaa ctgtgggagc ctcaccatcc
      481 gggccccttg gggctgtgtg ggccatgggg ctctttacca ttctcagagt cctgaagcct
      541 tttttgccca ttgcccagga atcaaggtgg ttatacccag aagccctttc caggccaagg
      601 gacttctgtt gtcatgcata gaagataaaa atccatgtat attttttgaa cctaagatac
      661 tttaccgggc agcagtggaa caggtcccag tagaacccta cagaatcccc ttgtctcagg
      721 ccgaagtcat ccaggagggc agcgatgtga ctctggttgc ctggggcact caggttcatg
      781 tcatccggga ggtggcttcc atggcccaag aaaagcttgg agtgtcttgt gaagtcatcg
      841 atctgacgac cattgtgcct tgggatgtgg atacagtttg taagtctgtg atcaaaacgg
      901 ggcgactgtt gatcagccac gaggcgccct tgacaggcgg ctttgcctct gagatcagct
      961 ccacggtcca ggaagaatgt ttcttgaacc tagaggctcc aatatctaga gtgtgtggat
     1021 atgacacacc atttcctcac atttttgaac ccttctatat cccagacaaa tggaagtgct
     1081 atgatgccct tcgcaagatg atcaactact gacgactgag aaaaccagaa gatcatgacc
     1141 agatatggaa atatttttct gaactctttt tatatttcct tgtacttctt tcttctcaca
     1201 cgtggttttg tgagatgaac tatggcggat actggctgac gagctgcgaa ttacttatca
     1261 tgctgtgtaa agtgattcct ggatccagtg catgaggtaa cgctttagag gcacatggcg
     1321 gtgatcttcg attgtttcat gactacaaat atccggtggt gttgtgttca ataaagcaaa
     1381 atgg
//