From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: D.pseudoobscura Adh gene for alcohol dehydrogenase
Date: 28 Dec 92 21:27:27 GMT
Distribution: bionet
Checksum: 23384 8

LOCUS       DROADHGN51   3465 bp ds-DNA             INV       01-DEC-1992
DEFINITION  D.pseudoobscura Adh gene for alcohol dehydrogenase
ACCESSION   X62231
KEYWORDS    ADH gene; alcohol dehydrogenase.
SOURCE      Drosophila pseudoobscura DNA.
  ORGANISM  Drosophila pseudoobscura
            Eukaryota; Animalia; Metazoa; Arthropoda; Uniramia; Insecta;
            Pterygota; Neoptera; Holometabola; Diptera; Brachycera;
            Cyclorrhapha; Schizophora; Drosophiloidea; Drosophilidae.
REFERENCE   1  (bases 1 to 3465)
  AUTHORS   Schaeffer,S.W. and Miller,E.L.
  TITLE     Estimates of gene flow in Drosophila pseudoobscura determined from
            nucleotide sequence analysis of the alcohol dehydrogenase region
  JOURNAL   Genetics 132, 471-480 (1992)
  STANDARD  full automatic
COMMENT     *source: strain=PS102; *source: isolate=Stemwinder Provincial Park,
            British Columbia, *source: Canada; *source: chromosome=IV section
            88;
               From EMBL    entry DPADHGN51;  dated 16-OCT-1992.
FEATURES             Location/Qualifiers
     mRNA            join(40..119,915..1020,1084..1488,1549..1964)
                     /note="adult mRNA (Adh)"
     mRNA            join(882..1020,1084..1488,1549..1964)
                     /note="larval mRNA (Adh)"
     promoter        8..14
                     /note="adult promoter (Adh)"
     prim_transcript 40..1964
                     /note="adult primary transcript (Adh)"
     exon            40..119
                     /note="adult exon 1a"
     intron          120..914
                     /note="adult intron"
     promoter        846..853
                     /note="larval promoter (Adh)"
     prim_transcript 882..1964
                     /note="larval primary transcript (Adh)"
     exon            882..1020
                     /note="larval exon 1"
     exon            915..1020
                     /note="adult exon 1b"
     intron          1021..1083
                     /number=1
     exon            1084..1488
                     /number=2
     intron          1489..1548
                     /number=2
     exon            1549..1964
                     /number=3
     polyA_signal    1941..1946
     polyA_site      1964
     CDS             join(928..1020,1084..1488,1549..1815)
                     /EC_number="1.1.1.1"
                     /gene="Adh"
                     /product="alcoholdehydrogenase"
                     /codon_start=1
                     /translation="MSLTNKNVVFVAGLGGIGLDTSRELVKRNLKNLVILDRIDNPAA
                     IAELKAINPKVTITFYPYDVTVPVAETTKLLKTIFAQVKTIDVLINGAGILDDHQIER
                     TIAVNYTGLVNTTTAILDFWDKRKGGPGGIICNIGSVTGFNAIYQVPVYSGSKAAVVN
                     FTSSLAKLAPITGVTAYTVNPGITKTTLVHKFNSWLDVEPRVAEKLLEHPTQTSQQCA
                     ENFVKAIELNKNGAIWKLDLGTLEPITWTQHWDSGI"
BASE COUNT      965 a    789 c    800 g    911 t
ORIGIN      
        1 cgcagtgtat ttaaggcact gcacatgtcg aggaccacga ttattgtcta cagtgcagtc
       61 agtagttgtc aggtgcagca ggcggaccag catttcttta aatctactca atcgatcaag
      121 taagtaactg taatccaagt acagtcggcc catcccactc ggttctgccg tagctcaaga
      181 aatttcttga tagcgcagtt ccagttgtaa aacgtcgttt aaaaaaacat ctcgttggag
      241 cttgggttgg aaaacgttcg agttgggcgt agaaaatttc agatataaaa tatcaaataa
      301 gagaaaatat tctcccaaag cagctgtcca ttttttgtta aagtgataga tcaaaaattc
      361 gtatgacgta tttgccaagt gctctttatg caaataattt cctcaattct aattctacaa
      421 gaagcacttt tggctcttgt ccagaaaaaa caatttgttt acactttgcg gttgataaca
      481 gaagcggacc gacggctaag taacataaca gtcgcctgtt aagtaatgcc ctcactcgct
      541 gttagatggc cgcgcgtgtc gactccgata gaccagcaga cacgcagtag ttttcctcaa
      601 gaacctgaac tctaaacata gacataattt acgatacctt gagactcaaa taaaaataca
      661 tgtatgagtg gtattctatt cctgattaga ctcgcacaca cacacgtacg agtattaaca
      721 taaatttgcc ggcaaataac caaaacaaaa cgaacgcaaa cgatccgaac acgtaatgcg
      781 agagataaga aacagaaaag ctcgacgtga aagaagcttc tggcggaagc tcttgccgaa
      841 ctccctataa atacgagaca gaaaaccaga ataaatataa taacaaactg ttgaattgtc
      901 ccatcgattc ccagatcaaa agacagaatg tctctcacca acaagaacgt cgttttcgtg
      961 gccggcctgg gcggcattgg cctggacacc agccgggagt tggtcaagcg taatctgaag
     1021 gtaagagaga acgaattcca tggaatctat ggaatcctaa atttaaaaat tcatttattt
     1081 tagaacctgg tcatcctgga tcgcattgac aatccggctg ccattgccga actgaaggca
     1141 atcaatccca aggtgaccat caccttctat ccctacgatg tgactgtgcc cgtcgctgag
     1201 accaccaagc tcctgaagac catctttgcc caggtgaaga caatcgatgt cctgatcaac
     1261 ggtgctggca tcctggacga tcatcagatc gagcgcacca ttgccgttaa ctacacgggc
     1321 ctggtcaaca ccaccacagc cattctggac ttctgggaca agcgcaaggg cggcccaggc
     1381 ggcatcattt gcaacattgg ctccgtcacc ggtttcaatg ccatctacca ggtgcccgtt
     1441 tactccggct ccaaggcggc ggtggttaac tttacctcct ccctggcggt aagtaatcca
     1501 acgtttttcc tactccccaa aactaaatat ttctttgaaa tcccatagaa actggctccc
     1561 attactggtg tcactgcata cactgtcaat cctggcatca ctaagaccac tctggtccac
     1621 aaattcaact cgtggctgga tgtggagccc cgtgtggcgg agaagctgct cgagcatccc
     1681 acccagacct ctcagcagtg cgccgagaac tttgtgaagg ccattgagct gaacaagaac
     1741 ggtgccatct ggaagttgga tttgggcacc ttggagccca tcacatggac ccagcactgg
     1801 gactcgggca tctaaaccat cccagagact ctatgggaca tggcgtttta gcttttagtt
     1861 tctttttttc cactcaattg ttacgtatat acatacacat atggcaataa ggctgatttg
     1921 aaccccgttt tgaatatgat aatacaaaat tatatttgag aaatttcaac aaaatcgata
     1981 agagctatag ctctctttta attaaaaaaa actagataaa aagagcaatg gtcgtcagtg
     2041 gtggtcagtg gtcgttgtgg cctctatcga tttccacaca aaaactttac tgtttagtaa
     2101 tagaaaagaa cttgagaaag gcagccaaaa gaatgtacga tctgacgggt aagcatgtct
     2161 gctatgtggc tgactgcggt ggcattgcac tggagaccag caaggttctc atgaccaaga
     2221 atatagcggt gagtggtaga gtggtggtgt agagagtgga gagtggttct ggttcttaaa
     2281 caagtctcta gtctcttggc tgctgctggt agctgtcgaa actaattaag ttaatgactt
     2341 tttcatgttt tgtttttgtc cgctgacttt tgctgttcaa agctacaatt aagtttatgt
     2401 tttgagtttg atctactttt gcttaaggga agtactttcg attacggatt gctggcatat
     2461 aaaaatagat accaaggaat gtgcatcata tcatattgat tctatcttga ttctttgccc
     2521 agaaactggc catccttcag agcgtggaaa accccccggc catcgcccag ttacaatcga
     2581 ttaagcacag cacacagatc ttcttctgga ccttcgatgt gaccatggcc cgcgaggaga
     2641 tgaagaagta cttcgatgag gtcatggtcc agatggacta catcgatgta ctgatcaatg
     2701 gggccaccct gtgcgatgag cggaacattg atgccaccat caatacgaat ctgaccggca
     2761 tgatgaacac cgtggccact gtgctgccct atatggaccg taagatgggc ggttcgggtg
     2821 gactgattgt gaatgtcacc tccgtcatag gactggatcc atcgccggtc ttttgtgcgt
     2881 acagtgcctc aaagtttggt gtgattgggt tcacccgaag tctagcggtg agtgggcgag
     2941 atcttaactt gaaaatcctc tctaatgtct tgtattcttt cgcaggatcc cctgtattac
     3001 acccaaaatg gtgtggctgt catggccgtc tgctgtggtc ccaccaaagt gtttgtcgat
     3061 cgggaactga ctgccttcct ggaatacggc cagtcctttg ccgatcgcct gcgtcgtgcc
     3121 ccctgccaat cgacggccgt ctgcggcaag aatatagtaa atgccatcga gagatcggag
     3181 aacggacaga tctggattgc cgacaagggc ggcctggagt cggtggccct ccactggtat
     3241 tggcatatgg ccgatcagtt tgtgaactat atgcagagca ctgacgatga ggatcaggag
     3301 ttttttttgg gccagcgatg aggcatgcct tgatccacgg aataaaacat caaatgtgtc
     3361 acaaaaagta ggcaacttgt ttgtgggtct ggaaatagat ttgaaatatt ttgaggtcat
     3421 atgaataatg acataaatga gggtgcctga aagtatgcca agttt
//