From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Didelphis virginiana alpha-1 proteinase inhibitor mRNA, complete
Date: 21 Dec 92 20:50:24 GMT
Distribution: bionet
Checksum: 44558 4

LOCUS       OPOSERPIN    1349 bp ss-mRNA            MAM       20-NOV-1992
DEFINITION  Didelphis virginiana alpha-1 proteinase inhibitor mRNA, complete
            cds.
ACCESSION   L06824
KEYWORDS    alpha-1 proteinase inhibitor; alpha-1-antitrypsin; serpin.
SOURCE      Didelphis virginiana (library: of L.Kress) liver cDNA to mRNA.
  ORGANISM  Didelphis virginiana
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Metatheria; Marsupialia; Marsupicarnivora; Didelphidae.
REFERENCE   1  (bases 1 to 1349)
  AUTHORS   Catanese,J.J. and Kress,L.F.
  TITLE     Opossum serum alpha-1-proteinase inhibitor: purification, linear
            sequence and resistance to inactivation by rattlesnake venom
            metalloproteinases
  JOURNAL   Biochemistry (1993) In press
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     sig_peptide     43..105
                     /note="putative"
                     /codon_start=1
     mat_peptide     106..1272
                     /function="inhibits neutrophil elastase, trypsin and
                     chymotrypsin"
                     /standard_name="alpha-1-proteinase inhibitor"
                     /product="alpha-1 proteinase inhibitor"
                     /codon_start=1
     polyA_signal    1327..1332
     CDS             43..1275
                     /function="Inhibits neutrophil elastase, trypsin, and
                     chymotrypsin"
                     /note="putative"
                     /label=serpin
                     /product="alpha-1 proteinase inhibitor"
                     /codon_start=1
                     /translation="MMPSTLSLCLMLAGLCSLVTSHLTEEIQASNDTENEYSSTRRIS
                     PYMTDFSIDFYRLLVSKSNTTNIFFSPISIYTAFTLLALGAKSATRDQILTGLRFNRT
                     EISEEHIFEGFQQLLNTFNLPENELQLTTSNGLFIDKNLKLVAKFLEDSKRLYASDTF
                     STNFEDNMAAKKQINDYVEKETQGKIVDLIQNLDSNVVFVLVNCIFFKGKWEKPFMTE
                     LTTECPFHVDSKTTVPVQTMRRLGMFNVFYDQDLSCWVLKMKYMGNATALFILPDTGK
                     IEKVENALNKMLFHKWTRNLKRRAISLYFPKVSISGNYDLKILRELGITDVFGSNADL
                     SGITEETNLKLSQAVHKAVVNIDEKGTEASGATFAEGIPMSIPPTVEFLRPFIFIILE
                     ENTKSVLFMGKVMNPTGN"
BASE COUNT      413 a    295 c    274 g    367 t
ORIGIN      
        1 gctgtcctac cgagtttctg caggagtctg aagaaggatc taatgatgcc ttctactctg
       61 tcactctgcc tgatgttggc tgggctatgc agcctggtca ccagccacct aactgaggaa
      121 atccaagcca gtaatgatac tgaaaatgag tattcttcca ccaggagaat ttccccttat
      181 atgactgatt ttagcatcga tttttataga ctgctggtgt ctaagtccaa taccaccaac
      241 atattcttct ctcctataag tatttacact gcctttacct tgctggctct tggggccaaa
      301 tcagcaactc gtgatcagat cctgacgggg ttaagattca accgtactga gatttcagag
      361 gaacatattt ttgaaggctt ccaacagctt cttaatacat tcaacctacc tgaaaatgag
      421 cttcaattga ccacaagcaa tggcctgttt atagacaaaa atctgaaact tgtagctaaa
      481 tttctagaag acagcaaaag actatatgct tctgatacct tctctacaaa ttttgaagac
      541 aacatggctg ccaagaaaca gatcaatgac tatgtagaga aggagaccca aggaaaaata
      601 gtggatttga ttcaaaacct ggactctaat gtcgtctttg tcctggtgaa ttgcattttc
      661 tttaaaggca agtgggagaa acccttcatg acagagctca ctacggaatg ccccttccac
      721 gtggattcca aaacaactgt gccagttcaa acaatgagac gccttggcat gttcaatgtg
      781 ttttatgatc aggacctgtc ctgctgggta ttgaaaatga aatatatggg aaacgcaact
      841 gcccttttca tactgccaga cacagggaag atagaaaaag tggagaatgc cctgaacaaa
      901 atgctgtttc acaaatggac acgcaactta aagcgcaggg caatcagttt atattttcca
      961 aaagtttcca tctcaggaaa ctatgatctg aagatactac gtgaactggg aatcacagat
     1021 gtatttggaa gtaatgcgga tctctctgga atcacagagg aaacaaacct taagctttcc
     1081 caggctgtgc acaaagctgt ggtgaacatt gatgagaaag gaacagaggc ttcaggagcc
     1141 actttcgcag aaggaatacc tatgtccatt ccccctaccg tggaattctt gaggcctttt
     1201 atatttataa ttttggaaga aaacacaaag agtgtacttt tcatgggaaa agttatgaat
     1261 cctactggaa attagcatct gcagtctcag cccctcaaga tctccgtgtt ctctcgtctc
     1321 ctgcagaata aagaagcagc atccatctc
//