From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: L.esculentum gene for polyphenol oxidase.
Date: 25 Jul 92 10:07:03 GMT
Distribution: bionet
Checksum: 60893     6

LOCUS       TOMPOOXB     2101 bp ds-DNA             PLN       25-JUL-1992
DEFINITION  L.esculentum gene for polyphenol oxidase.
ACCESSION   Z12834
KEYWORDS    polyphenol oxidase.
SOURCE      Lycopersicon esculentum DNA.
  ORGANISM  Lycopersicon esculentum
            Eukaryota; Plantae; Embryobionta; Magnoliophyta; Magnoliopsida;
            Asteridae; Solanales; Solanaceae.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Newman,S.M.
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
REFERENCE   2  (bases 1 to 2101)
  AUTHORS   Newman,S.M., Eannetta,N.T., Yu,H., Prince,J.P., de Vicente,C.,
            Tanksley,S.D. and Steffens,J.C.
  TITLE     Organization of the tomato polyphenol oxidase gene family
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
COMMENT     *source: strain=cv. VFNT cherry; *source: clone_library=Charon 35
            library of R. Fischer; *source: chromosome=8; *source:
            is_macronuclear=N; *source: is_proviral=N; *source: is_germline=N;
            From EMBL    entry LEPOOXB;  dated 26-JUN-1992.
FEATURES             Location/Qualifiers
     CAAT_signal     4..9
     TATA_signal     99..103
     CDS             151..1941
                     /product="polyphenol oxidase precursor"
                     /codon_start=1
                     /translation="MASVVCNSSSSTTTTTLKTPFTSLGSTPKPSQLFLHGKRNKTFK
                     VSCKVINNNGNQDETNSVDRRNVLLGLGGLYGVANAIPLAASATPIPSPDLKTCGRAT
                     ISDGPLVPYSCCPPPMPTNFDTIPYYKFPSMTKLRIRTPAHAVDEEYIAKYNLAISRM
                     RDLDKTEPLNPLGFKQQANIHCAYCNGAYIIGGKELQVHNSWLFFPFHRWYLYFYERI
                     LGKLIDDPTFALPYWNWDHPKGMRLPPMFDREGSSLYDERRNQQVRNGTVLDLGSFGD
                     KVETTQLQLMSNNLTLMYRQMVTNAPCPLLFFGAPYVLGNNVEAPGTIETIPHIPVHI
                     WAGTVRGSKFPNGDVSYGEDMGNFYSAGLDPVFYCHHGNVDRMWNEWKAIGGKRRDIS
                     EKDWLNSEFFFYDEHKNPYRVKVRDCLDTKKMGYDYAPMPTPWRNFKPKSKASVGKVN
                     TSTLPPANEVFPLAKMDKTISFAINRPASSRTQQEKNEQEEMLTFNNIRYDNRGYIRF
                     DVFLNVDNNVNANELDKAEFAGSYTSLPHVHRAGENDHIAKVNFQLAITELLEDIGLE
                     DEDTIAVTLVPKKGGEGISIENVEIKLVDC"
     transit_peptide 151..411
     mat_peptide     412..1938
                     /product="polyphenol oxidase"
     misc_binding    745..855
                     /bound_moiety="copper, tyrosinase-like CuA bindingsite"
     misc_binding    1134..1320
                     /bound_moiety="copper, tyrosinase-like CuB bindingsite"
BASE COUNT      607 a    427 c    444 g    623 t
ORIGIN
        1 actcaaaatt attactatct caatctctcc cacctaccgc atgtatatgt tggagtgtta
       61 ggtgagcctc taaactttta cttactagca acatattata taatgcaagg acttggaacc
      121 aaataattca tcaaccaaaa gctattagca atggcaagtg tagtgtgcaa tagtagtagt
      181 agtactacta ctacaacgct caaaactcct tttacttctt taggttccac tcctaagccc
      241 tctcaacttt tccttcatgg aaaacgtaac aaaacattca aagtttcatg caaggttatc
      301 aataataacg gtaaccaaga tgaaacgaat tctgttgatc gaaggaatgt tcttcttggt
      361 ttaggaggtc tttatggtgt tgctaatgct ataccattag cggcatcggc tactcctatt
      421 ccatcccctg atctcaaaac ttgtggtaga gccaccatat cggatggtcc acttgtaccc
      481 tattcttgtt gtccccctcc tatgccgact aactttgaca ccattccata ttacaagttc
      541 ccttctatga ctaaactccg tatccgtacc cctgctcatg ctgtagatga ggagtatatc
      601 gcgaagtata atttggccat aagtcgaatg agggatcttg acaagacaga accgttaaac
      661 cctctagggt ttaagcaaca agctaatata cactgtgctt attgtaacgg tgcttatata
      721 attggtggca aagagttaca agttcataac tcgtggcttt tcttcccgtt ccatcgatgg
      781 tacttgtact tttacgaaag aatattgggg aaactcattg atgatccaac tttcgcttta
      841 ccatactgga attgggatca tccaaagggc atgcgtttac ctcccatgtt cgatcgtgaa
      901 ggttcttccc tctacgatga aaggcgtaat caacaagtcc gtaatggaac ggttttggat
      961 cttggttcat ttggggataa agttgaaaca actcaactcc agttgatgag caataattta
     1021 accctaatgt accgtcaaat ggtaactaat gctccatgtc ctctcttgtt cttcggtgcg
     1081 ccttacgttc ttgggaataa cgttgaagca ccgggaacca ttgaaaccat ccctcatatt
     1141 cctgtacata tttgggctgg tactgtccgt ggttcaaaat ttcctaacgg tgatgtgtcc
     1201 tacggtgagg atatgggtaa tttctactca gctggtttgg acccggtttt ctattgccat
     1261 cacggcaatg tggaccggat gtggaacgaa tggaaggcaa taggaggtaa aagaagagat
     1321 atatctgaaa aggattggtt gaactccgag ttctttttct acgacgaaca caaaaatcct
     1381 taccgtgtga aagtcaggga ctgtttggac acgaagaaaa tggggtatga ttacgcacca
     1441 atgccaactc catggcgtaa tttcaaacca aaatcaaagg cgtccgtagg gaaagtgaat
     1501 acaagtacac tccccccagc aaacgaggta ttcccactcg cgaagatgga taagactatt
     1561 tcatttgcta tcaacaggcc agcttcatcg cggactcaac aagagaaaaa tgaacaagag
     1621 gagatgttaa cgttcaataa cataagatat gataacagag ggtacataag gttcgatgtg
     1681 ttcctgaacg tggacaacaa tgtgaacgcg aatgagcttg ataaggcaga gttcgcgggg
     1741 agttatacta gtttgccaca tgttcacaga gctggcgaga atgatcatat cgcgaaggtt
     1801 aatttccagc tggcgataac agaactgttg gaggacattg gtttggaaga tgaagatact
     1861 atcgcggtga ctctggtacc aaagaaaggc ggtgaaggta tctccattga gaatgtggag
     1921 atcaagcttg tggattgtta agtctcaatt gaatttgctg agattacaat tatgatggat
     1981 gatgatatgt ttttatgtta cttttgttct gttatctact tttgcttttc tcgtgtaact
     2041 tttcctgttg aaatcagcct acatgcttga tttccttgga gttgttattc actaataaat
     2101 c
//