From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: E.coli D-serine deaminase activator (dsdC) gene, complete cds, and
Date: 10 Sep 92 10:31:48 GMT
Distribution: bionet
Checksum: 51101     7

LOCUS       ECODSDAA     1913 bp ds-DNA             BCT       10-SEP-1992
DEFINITION  E.coli D-serine deaminase activator (dsdC) gene, complete cds, and
            D-serine deaminase (dsdA) gene, 5' end.
ACCESSION   M19035
KEYWORDS    D-serine deaminase; D-serine deaminase activator;
            regulatory protein.
SOURCE      E.coli DNA, clone pAC131.
  ORGANISM  Escherichia coli
            Prokaryotae; Gram-negative facultatively anaerobic rods;
            Enterobacteriaceae.
REFERENCE   1  (bases 1 to 1913)
  AUTHORS   Palchaudhuri,S., Patel,V. and McFall,E.
  TITLE     DNA sequence of the D-serine deaminase activator gene dsdC
  JOURNAL   J. Bacteriol. 170, 330-334 (1988)
  STANDARD  simple staff_review
REFERENCE   2  (bases 1 to 1913)
  AUTHORS   McFall,E., Nikam,S.S. and Palchaudhuri,S.
  TITLE     Effects of structural changes in the dsdC Intergenic region on
            D-Serine deaminase synthesis
  JOURNAL   J. Bacteriol. 173, 1161-1167 (1991)
  STANDARD  simple staff_review
COMMENT     The authors are re-examining certain portions of this sequence.
            They have reason to question the accuracy of base pairs 1210 to
            1610 (-1130 to -1530 in journal), and have concluded that base
            pairs 1380 to 1430 (-1300 to -1430 in journal) are certainly in
            error.  The corrected sequence is being sought at the authors'
            request by Dr. James Brannigan, at the Protein Structural Research
            Laboratory, University of York.
            J. Bacteriol. 170, 330-334 (1988) reports the sequence from bp
            281-1913.  J. Bacteriol. 173, 1161-1167 (1991) reports the sequence
            from bp 1-840.
FEATURES             Location/Qualifiers
     mRNA            complement(<1..84)
                     /gene="dsdA"
     CDS             complement(<1..3)
                     /product="D-serine deaminase"
                     /gene="dsdA"
                     /codon_start=1
                     /translation="M"
     CDS             545..1564
                     /note="ORF"
                     /codon_start=1
                     /translation="MAVIGLPINSNQDIQTTLTRHIQFGNRFNQYSAQWVAVWRITLR
                     LMTVVVFSSRTLRSANSVTRPGSPDYQRRPVVTTESDTGKARHVHVMQLHSHLSPTSK
                     CRDALSSSQNRASLESALVTYLRYNKHYRASLLVMRNHRVRIGHQVRYIAEWQALIMR
                     YWFFWQLKKPEESIARRPLQQTRDATNQANQHHNDITESQRQRMKCQTNSFCGPGYFT
                     IFPISARLIAKRTCPERSERNAIKCELAHSLGPDFHTLCFRLLCVCWNCERITISHRK
                     RLVTMTYAKLGHACRSTLEDPPSNSARNGWNLNFTMVSTTYTMLISTACSHPDFESAL
                     SHLPL"
     CDS             770..1564
                     /note="putative alternate; gtg start codon"
                     /product="D-serine deaminase activator"
                     /gene="dsdC"
                     /note="putative"
                     /codon_start=1
                     /translation="MTTESDTGKARHVHVMQLHSHLSPTSKCRDALSSSQNRASLESA
                     LVTYLRYNKHYRASLLVMRNHRVRIGHQVRYIAEWQALIMRYWFFWQLKKPEESIARR
                     PLQQTRDATNQANQHHNDITESQRQRMKCQTNSFCGPGYFTIFPISARLIAKRTCPER
                     SERNAIKCELAHSLGPDFHTLCFRLLCVCWNCERITISHRKRLVTMTYAKLGHACRST
                     LEDPPSNSARNGWNLNFTMVSTTYTMLISTACSHPDFESALSHLPL"
     CDS             815..1564
                     /product="D-serine deaminase activator"
                     /gene="dsdC"
                     /codon_start=1
                     /translation="MQLHSHLSPTSKCRDALSSSQNRASLESALVTYLRYNKHYRASL
                     LVMRNHRVRIGHQVRYIAEWQALIMRYWFFWQLKKPEESIARRPLQQTRDATNQANQH
                     HNDITESQRQRMKCQTNSFCGPGYFTIFPISARLIAKRTCPERSERNAIKCELAHSLG
                     PDFHTLCFRLLCVCWNCERITISHRKRLVTMTYAKLGHACRSTLEDPPSNSARNGWNL
                     NFTMVSTTYTMLISTACSHPDFESALSHLPL"
     misc_feature    1248..1486
                     /note="putative VECTOR sequence M13mp19"
                     /note="putative"
     terminator      1708..1729
                     /note="potential transcription terminator"
                     /note="putative"
BASE COUNT      563 a    466 c    439 g    445 t
ORIGIN      19 bp upstream of SphI site; 51 min on K12 map.
        1 catagtacgt ctctttgcgc ttagatgata aatgacagca ggaatgtgcc cgccagagcg
       61 acgactgaag cgataaatgt cgctgtcgta tagtatttaa atgtttcatt gagcgtagcg
      121 ccgcaatatt gcttcactag ccagaaaagc gagtccgtaa cgatagtgca gccaattgca
      181 cctgaaccga tagcaatcgc aataatttcc gggctgatgt cgggatacag cggcagcatg
      241 ggtgcaacaa ttgccgttgc ccccatcatt gccacggtag cggagcccac tgccgcatgc
      301 agaataagag ccactaacca ggccagaaga atcgggtgca tatgcatatt ggagagaata
      361 actgccagcg tatcagcgag actgctgctt tttaaaatgc gttgaatgcg cctccggccc
      421 cgataatcag caaaatatta gcaatagaac cgaagccatt ttccgtatgt gtgagcatcg
      481 tccccatgct catatgctgg accgtatacc caacacataa taggcgacaa acacggcgat
      541 aaacatggca gtgatagggt tgccaataaa ctccaaccaa gatatacaaa ccactctcac
      601 gcgccatatt caattcggca atcgttttaa ccaatactca gcgcaatggg tagcagtatg
      661 gagaataacg ttgcgcctaa tgacggtagt ggttttttca tcgcgaactt tgagatctgc
      721 aaactctgta actcgtccgg gttcaccaga ttaccaacgg cggccagtcg tgaccactga
      781 aagtgacacg ggaaaagcta ggcacgtaca cgtaatgcag ctgcatagcc atctaagccc
      841 aaccagcaaa tgccgtgacg cactgagctc tagccagaat cgcgcatcac tagagtcagc
      901 cctagtgact tatcttaggt acaacaaaca ctatagagca agcctactcg tgatgcgcaa
      961 ccaccgagta cgcatcggcc atcaagtgcg gtacatagcg gaatggcaag ctttaataat
     1021 gaggtattgg tttttttggc aattgaaaaa gccagaggaa tcaatagcac gacgcccact
     1081 tcaacaaaca cgtgatgcca caaatcaggc caaccagcac cataatgaca tcactgaaag
     1141 ccagcggcag cgtatgaagt gtcagaccaa ttctttctgc ggccccggat acttcactat
     1201 ttttccgatt agcgctaggc taatcgcgaa acggacttgc cctgaacgga gtgagcgcaa
     1261 cgcaattaaa tgtgagttag ctcactcatt aggcccagac ttccatactt tatgcttccg
     1321 gctgctatgt gtgtgttgga attgtgagcg gataacaatt tcacacagga aacgactggt
     1381 gaccatgact tacgccaagc ttgggcatgc ctgcaggtcg actctagagg atccccccag
     1441 caacagcgcc agaaacgggt ggaatttgaa cttcacgatg gtcagtacta cttacacgat
     1501 gctgataagc actgcgtgct cacacccaga ttttgagagt gcattatctc accttcccct
     1561 gtgattaacc tgatgatatt ggacactaaa aacaacgtag gctgacaacg ataaattgtc
     1621 cttcacatga ggtaaagatg catcaatgga gtgattacat ctaaaaaatg aaggtgaatt
     1681 gagatatggt tcactttagc tcatctcata gctgaaattt tgccgctatt tttttacact
     1741 taagcgaaaa aagtgaggtg attatggaac cccttcgtga aataagaaat cggctgctta
     1801 acgctggcaa ttatcaaaaa atgcatactt ttgaagtgga ctgccaggca tcagtccttc
     1861 gcctggcggc agaggaatta agtcgctgag ccccatgcgt aagtcacctt aag
//