From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Escherichia coli alpha-D-glucosyl-1-phosphate uridylyltransferase
Date: 15 Aug 92 10:01:36 GMT
Distribution: bionet
Checksum: 31685     5

LOCUS       ECOGALU      1288 bp ds-DNA             BCT       15-AUG-1992
DEFINITION  Escherichia coli alpha-D-glucosyl-1-phosphate uridylyltransferase
            (galU) gene, complete cds.
ACCESSION   M98830
KEYWORDS    alpha-D-glucosyl-1-phosphate uridylyltransferase; galU gene;
            glucophosphate uridylyltransferase.
SOURCE      Escherichia coli (strain K-12) DNA.
  ORGANISM  Escherichia coli
            Prokaryotae; Gram-negative facultatively anaerobic rods;
            Enterobacteriaceae.
REFERENCE   1  (bases 1 to 1288)
  AUTHORS   Pon,C.L., Calogero,R.A. and Gualerzi,C.O.
  TITLE     Identification, cloning, nucleotide sequence and chromosomal map
            location of hns, the structural gene for Escherichia coli
            DNA-binding protein H-NS
  JOURNAL   Mol. Gen. Genet. 212, 199-202 (1988)
  STANDARD  full staff_entry
REFERENCE   2  (bases 1 to 1288)
  AUTHORS   Ueguchi,C. and Ito,K.
  TITLE     Multicopy suppression: an approach to understanding intracellular
            functioning of the protein export system
  JOURNAL   J. Bacteriol. 174, 1454-1461 (1992)
  STANDARD  full staff_entry
REFERENCE   3  (bases 1 to 1288)
  AUTHORS   Weissborn,A.C., Rumley,M.K. and Kennedy,E.P.
  TITLE     The galU gene of Escherichia coli
  JOURNAL   Unpublished (1993)
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     RBS             222..226
                     /standard_name="Shine-Dalgarno sequence"
                     /gene="galU"
                     /note="putative"
                     /citation=[3]
     CDS             234..1142
                     /function="synthesizes UDPG"
                     /EC_number="2.7.7.9"
                     /EC_number="2.7.7.9"
                     /standard_name="alpha-D-glucosyl-1-phosphate
                     uridylyltransferase"
                     /product="glucosephosphate uridylyltransferase"
                     /gene="galU"
                     /evidence=EXPERIMENTAL
                     /codon_start=1
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
                     /citation=[3]
     conflict        replace(1180..1186,"attgaca")
                     /gene="galU"
                     /citation=[1]
     conflict        replace(1240..1250,"aatccgccgc")
                     /gene="galU"
                     /citation=[1]
BASE COUNT      358 a    262 c    327 g    341 t
ORIGIN
        1 gtcgactgcg cttgatgttg tctgcagaat gagcaaacga taacgcgggc taaatttgca
       61 ttacctgcta atgtcggctg gtggtactat cgtcgccatt cgtataagta attgtcttaa
      121 ttatgctaac tcgcctcctt ttcagaactt agccccttcg gggtgctgat atactgggat
      181 gcgatacaga aatatgaaca cgttcaaaac acgaacagtc caggagaatt taaatggctg
      241 ccattaatac gaaagtcaaa aaagccgtta tccccgttgc gggattagga accaggatgt
      301 tgccggcgac gaaagccatc ccgaaagaga tgctgccact tgtcgataag ccattaattc
      361 aatacgtcgt gaatgaatgt attgcggctg gcattactga aattgtgctg gttacacact
      421 catctaaaaa ctctattgaa aaccactttg ataccagttt tgaactggaa gcaatgctgg
      481 aaaaacgtgt aaaacgtcaa ctgcttgatg aagtgcagtc tatttgtcca ccgcacgtga
      541 ctattatgca agttcgtcag ggtctggcga aaggcctggg acacgcggta ttgtgtgctc
      601 acccggtagt gggtgatgaa ccggtagctg ttattttgcc tgatgttatt ctggatgaat
      661 atgaatccga tttgtcacag gataacctgg cagagatgat ccgccgcttt gatgaaacgg
      721 gtcatagcca gatcatggtt gaaccggttg ctgatgtgac cgcatatggc gttgtggatt
      781 gcaaaggcgt tgaattagcg ccgggtgaaa gcgtaccgat ggttggtgtg gtagaaaaac
      841 cgaaagcgga tgttgcgccg tctaatctcg ctattgtggg tcgttacgta cttagcgcgg
      901 atatttggcc gttgctggca aaaacccctc cgggagctgg tgatgaaatt cagctcaccg
      961 acgcaattga tatgctgatc gaaaaagaaa cggtggaagc ctatcatatg aaagggaaga
     1021 gccatgactg cggtaataaa ttaggttaca tgcaggcctt cgttgaatac ggtattcgtc
     1081 ataacaccct tggcacggaa tttaaagcct ggcttgaaga agagatgggc attaagaagt
     1141 aacatccgta tcggtgttat ccacgaaacg gcgttgagca atcgacgccg tttttttata
     1201 gcttattctt attaaattgt cttaaaccgg acaataaaaa atcccgccgc tggcgggatt
     1261 ttaagcaagt gcaatctaca aaagatta
//