From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: S.cerevisiae GSH1 gene for gamma-glutamylcysteine synthetase.
Date: 5 Sep 92 10:02:14 GMT
Distribution: bionet
Checksum: 27414     9

LOCUS       YSCGSH1      4410 bp ds-DNA             PLN       05-SEP-1992
DEFINITION  S.cerevisiae GSH1 gene for gamma-glutamylcysteine synthetase.
ACCESSION   D90220 M87066
KEYWORDS    GSH1; gamma-glutamylcysteine synthetase.
SOURCE      S.cerevisiae (strain X2180) genomic DNA.
  ORGANISM  Saccharomyces cerevisiae
            Eukaryota; Fungi; Eumycota; Hemiascomycetes; Endomycetales;
            Saccharomycetaceae.
REFERENCE   1  (bases 1 to 4410)
  AUTHORS   Ohtake,Y. and Yabuuchi,S.
  TITLE     Molecular clonging of gamma-glutamylcysteine synthetase gene of
            Saccharomyces cerevisiae
  JOURNAL   Yeast 7, 953-961 (1991)
  STANDARD  full staff_review
COMMENT     These data kindly submitted in computer readable form by:
            Yasuyuki Ohtake
            Central Research Laboratories
            Asahi Breweries Ltds.
            2-13-1 Omori-kita
            Ota-ku, Tokyo 143
            Japan
            Phone:  03-5493-3250
            Fax:    03-5493-7027
FEATURES             Location/Qualifiers
     repeat_unit     579..587
                     /rpt_type=tandem
                     /note="repeat 1"
     repeat_unit     828..834
                     /rpt_type=tandem
                     /note="repeat 2"
     CAAT_signal     837..840
     CAAT_signal     976..979
     CAAT_signal     1010..1013
     TATA_signal     1068..1071
     CAAT_signal     1123..1126
     repeat_unit     1125..1133
                     /rpt_type=tandem
                     /note="repeat 1"
     TATA_signal     1131..1134
     repeat_unit     1147..1153
                     /rpt_type=tandem
                     /note="repeat 2"
     TATA_signal     1216..1219
     TATA_signal     1255..1258
     TATA_signal     1261..1264
     CDS             1275..3311
                     /EC_number="6.3.2.2"
                     /product="gamma-glutamylcysteine synthetase"
                     /codon_start=1
                     /translation="MGLLALGTPLQWFESRTYNEHIRDEGIEQLLYIFQAAGKRDNDP
                     LFWGDELEYMVVDFDDKERNSMLDVCHDKILTELNMEDSSLCEANDVSFHPEYGRYML
                     EATPASPYLNYVGSYVEVNMQKRRAIAEYKLSEYARQDSKNNLHVGSRSVPLTLTVFP
                     RMGCPDFINIKDPWNHKNAASRSLFLPDEVINRHVRFPNLTASIRTRRGEKVCMNVPM
                     YKDIATPETDDSIYDRDWFLPEDKEAKLASKPGFIYMDSMGFGMGCSCLQVTFQAPNI
                     NKARYLYDALVNFAPIMLAFSAAAPAFKGWLADQDVRWNVISGAVDDRTPKERGVAPL
                     LPKYNKNGFGGIAKDVQDKVLEIPKSRYSSVDLFLGGSKFFNRTYNDTNVPINEKVLG
                     RLLENDKAPLDYDLAKHFAHLYIRDPVSTFEELLNQDNKTSSNHFENIQSTNWQTLRF
                     KPPTQQATPDKKDSPGWRVEFRPFEVQLLDFENAAYSVLIYLIVDSILTFSDNINAYI
                     HMSKVWENMKIAHHRDAILFEKFHWKKSFRNDTDVETEDYSISEIFHNPENGIFPQFV
                     TPILCQKGFVTKDWKELKHSSKHERLYYYLKLISDRASGELPTTAKFFRNFVLQHPDY
                     KHDSKISKSINYDLLSTCDRLTHLDDSKGELTSFLGAEIAEYVKKNKPSIESKC"
     repeat_unit     3636..3658
                     /rpt_type=inverted
                     /note="repeat"
     polyA_signal    3736..3741
BASE COUNT     1284 a    862 c    917 g   1347 t
ORIGIN      Chromosome 10.
        1 gcatgcagcg gacgaaatac cgaatattga atctcatatt gacttccttc ttcttcttgc
       61 cctgacgctt tgtcctctgc attatgaagc agtccaacgg caggattgga ccctgaagtc
      121 tgatcaggtc gcgggctctc ggacggtacg ttgaccagag attgatagga agggtggaat
      181 acctgagggg cctccgctat gtctgaattc tgtgcattcg aaccgacccc tggagaagcg
      241 tgcgttatcg ggttcttact tttacagtga tcttgctcat cacggaactg taaccaatcg
      301 tagctgttca caactatctt ctggtgagga tttacggtat gatcatgctg gtaagcttcg
      361 ttactcatgc tttggttggc ttcttgaaag tgtctagttc actttatgcc cggttataaa
      421 cataaacttt cacagggttc tctttgatca cctctttgtt taatcttatg aatccaaggg
      481 attttatcgg tcaaagggga aatcaatgcg aaagacagta atgatgagag aaaaactctc
      541 cgtaaccacc aagtttggtt cagcgcgacg agatttttat cgattatcga gaaaaatacc
      601 tgtatatcta catttctatg tcagtgatat atacttctta gataagttat gccaccagtg
      661 catacgctta cgcacacaca cgtattcttg tgcacacgcc tgttacttct tgcagacatc
      721 agacatacta ttgtaattca aaaaaaaaaa gcgaatcttc ccatgcctgt tgctgctctt
      781 gaatggcgac agcctattgc cccagtgttc cctcaacaac cttggtagtt ggagcgcaat
      841 tagcgtatcc tgtaccatac taattctctt ctgcccaacg acggctgcca ttagtcagca
      901 tggcgcgcac gtgactacaa ctgtggctgg aaaccttttc gtcctccccg gtttttcagt
      961 gagccgactc tactacaatg ctttttcatt tttcactcag aaaaacctgc aatttgccaa
     1021 attggccatg ctctgtgcct cccttgacaa aggacatctt ccctgtttat aaacggcggc
     1081 ttaccaaaag ttgaagcttg ttcttgcctc ttatgagtgg agcaatcgat tatattgaat
     1141 cgttgtgctg gagtagttgg atctttccac gtggtctcga gtcacttgta gaagctgaaa
     1201 attgagcaga tttagtatag ggctacattg tagggtggtt tagagtatcg aaaatataca
     1261 tatagaagaa taaaatggga ctcttagctt tgggcacgcc tttgcagtgg tttgagtcta
     1321 ggacgtacaa tgaacacata agggatgaag gtatcgagca gttgttgtat attttccaag
     1381 ctgctggtaa aagagacaat gaccctcttt tttggggaga cgagcttgag tacatggttg
     1441 tagattttga tgataaggag agaaattcta tgctcgacgt ttgccatgac aagatactca
     1501 ctgagcttaa tatggaggat tcgtcccttt gtgaggctaa cgatgtgagt tttcaccctg
     1561 agtatggccg gtatatgtta gaggcaacac cagcttctcc atatttgaat tacgtgggta
     1621 gttacgttga ggttaacatg caaaaaagac gtgccattgc agaatataag ctatctgaat
     1681 atgcgagaca agatagtaaa aataacttgc atgtgggctc caggtctgtc cctttgacgc
     1741 tgactgtctt cccgaggatg ggatgccccg actttattaa cattaaggat ccgtggaatc
     1801 ataaaaatgc cgcttccagg tctctgtttt tacccgatga agtcattaac agacatgtca
     1861 ggtttcctaa cttgacagca tccatcagga ccaggcgtgg tgaaaaagtt tgcatgaatg
     1921 ttcccatgta taaagatata gctactccag aaacggatga ctccatctac gatcgagatt
     1981 ggtttttacc agaagacaaa gaggcgaaac tggcttccaa accgggtttc atttatatgg
     2041 attccatggg ttttggcatg ggctgttcgt gcttacaagt gacctttcag gcacccaata
     2101 tcaacaaggc acgttacctg tacgatgcat tagtgaattt tgcacctata atgctagcct
     2161 tctctgccgc tgcgcctgct tttaaaggtt ggctagccga ccaagatgtt cgttggaatg
     2221 tgatatctgg tgcggtggac gaccgtactc cgaaggaaag aggtgttgcg ccattactac
     2281 ccaaatacaa caagaacgga tttggaggca ttgccaaaga cgtacaagat aaagtccttg
     2341 aaataccaaa gtcaagatat agttcggttg atcttttctt gggtgggtcg aaatttttca
     2401 ataggactta taacgacaca aatgtaccta ttaatgaaaa agtattagga cgactactag
     2461 agaatgataa ggcgccactg gactatgatc ttgctaaaca ttttgcgcat ctctacataa
     2521 gagatccagt atctacattc gaagaactgt tgaatcagga caacaaaacg tcttcaaatc
     2581 actttgaaaa catccaaagt acaaattggc agacattacg ttttaaaccc cccacacaac
     2641 aagcaacccc ggacaaaaag gattctcctg gttggagagt ggaattcaga ccatttgaag
     2701 tgcaactatt agattttgag aacgctgcgt attccgtgct catatacttg attgtcgata
     2761 gcattttgac cttttccgat aatattaacg catatattca tatgtccaaa gtatgggaaa
     2821 atatgaagat agcccatcac agagatgcta tcctatttga aaaatttcat tggaaaaaat
     2881 catttcgcaa cgacaccgat gtggaaactg aagattattc tataagcgag attttccata
     2941 atccagagaa tggtatattt cctcaatttg ttacgccaat cctatgccaa aaagggtttg
     3001 taaccaaaga ttggaaagaa ttaaagcatt cttccaaaca cgagagacta tactattatt
     3061 taaagctaat ttctgataga gcaagcggtg aattgccaac aacagcaaaa ttctttagaa
     3121 attttgtact acaacatcca gattacaaac atgattcaaa aatttcaaag tcgatcaatt
     3181 atgatttgct ttctacgtgt gatagactta cccatttaga cgattcaaaa ggtgaattga
     3241 catccttttt aggagctgaa attgcagaat atgtaaaaaa aaataagcct tcaatagaaa
     3301 gcaaatgtta aactcctttt acttcggttg tgaaagaaag ttgacattat cgatttgggt
     3361 gacacggtga ttgaaaaagc aacgaccagt attatacctc ttttttttat tattcagttt
     3421 atatttttgc aagtgatctt aagcatttct acacaaactt atgccaacgt gaccatttat
     3481 tattttatat agcaaaaaaa aatgaggggc cttgcagaac aattgttgcg agtttctaat
     3541 aacaagcacg tagaatattg gccatttaat ttttctcttc aatttataga atggttgtgt
     3601 tagtgacaaa aagaatattc ttccccgcca ggactcgaac ctggaatctc ctggttcgta
     3661 gccagacgcc gtgaccattg ggccacgagg aacaagaata taaagatctc tgagggcaag
     3721 gtatgcctat gtcgcaataa aatgtttgtt cctgcgcaaa agtaaagttc tattaatata
     3781 caactacaca gttatcggtt cacactattc gatagttgta aaaaccattt tgataaagat
     3841 ataacaaggc gtttattaag gacatttttg ctacaagtcg tgaagtattg attgtaggcg
     3901 atcgttggta actttctcca tatcggaata ttcaatattg aactcacccc tcccttgcga
     3961 taagctcctt agcttattgg tgtaggtggt aatttccctt agtggcactt tcgcttttat
     4021 aatttttttt acattagaac tggtctcttg agtgtttttt ttatctttgg ttgcgtgtag
     4081 ggttgtaaca gcatcagagg gaatatatat cctattgctg ttctctggag aattacaagt
     4141 ggatgcgcca gaatttgaca cgcttgattc gtcttcaata gacagaattt gagcctttct
     4201 tgctcctgtt agatcttgta acaccgtacc aacatcagat tgagggattg ttagatcaag
     4261 attcatgatg ggttccagaa ggttctattt ttcaggtttt aagtcattga gagctttaaa
     4321 aattaagttt ctagtaattt ttaagatttc ttgtggagtt tcaatatcag gtgggactga
     4381 ccaatcgcta ttgattttga tggagcatgc
//