From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Plasmodium knowlesi erthrocyte binding protein (alpha) gene,
Date: 22 Aug 92 10:01:18 GMT
Distribution: bionet
Checksum: 50205    11

LOCUS       PFAERTHBPA   4745 bp ds-DNA             INV       22-AUG-1992
DEFINITION  Plasmodium knowlesi erthrocyte binding protein (alpha) gene,
            complete cds.
ACCESSION   M90466
KEYWORDS    erythrocyte binding protein.
SOURCE      Plasmodium knowlesi (strain Malayan H) late schizont DNA.
  ORGANISM  Plasmodium knowlesi
            Eukaryota; Animalia; Protozoa; Microspora; Microsporea;
            Microsporida; Haemosporina; Plasmodiidae.
REFERENCE   1  (sites)
  AUTHORS   Adams,J.H., Hudson,D.E., Torii,M., Ward,G.E., Wellems,T.E.,
            Aikawa,M. and Miller,L.H.
  TITLE     The Duffy receptor family of Plasmodium knowlesi is located within
            the micronemes of invasive malaria merozoites
  JOURNAL   Cell 63, 141-153 (1990)
  STANDARD  full staff_review
REFERENCE   2  (sites)
  AUTHORS   Fang,X., Kaslow,D.C., Adams,J.H. and Miller,L.H.
  TITLE     Cloning of the Plasmdium vivax receptor
  JOURNAL   Mol. Biochem. Parasitol. 44, 125-132 (1991)
  STANDARD  full staff_review
REFERENCE   3  (bases 1 to 4745)
  AUTHORS   Adams,J.H., Sim,B.K., Dolan,S.A., Fang,X., Kaslow,D.C. and
            Miller,L.H.
  TITLE     A family of erythrocyte binding proteins of malaria parasites
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 89, 7085-7089 (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     CDS             join(454..513,682..3643,3750..3828,4066..4142,4225..4268)
                     /product="erythrocyte binding protein"
                     /gene="alpha gene"
                     /codon_start=1
                     /translation="MEGKKKRPLFFLLVLLLSHKANNVLFERMNGILLLECENEYVKN
                     ENGYKLATGHHYMDNDQIEQWLQGTDRSRRVKIEENVKYKYNVEELNTKYEQMKAPRI
                     NRILKESTYEAQNVADNNYIDDKANGEHKTDNKTNKGEGARNMVMLDYDISGSGQPDG
                     IIDNVVELGTEDEGNFLENSSKGGDHPYRMNRKERMSNGVINQTFLQNNVMDKCNDKR
                     KRGERDWDCPAEKDICISVRRYQLCMKGLTNLVNNTRTHSHNDITFLKLNLKRKLMYD
                     AAVEGDLLLKKNNYQYNKEFCKDIRWGLGDFGDIIMGTNMEGIGYSQVVENNLRQVFG
                     TDEKAKQDRKQWWNESKEHIWRAMMFSIRSRLKEKFVWICKKDVTLKVEPQIYRWIRE
                     WGRDYMSKLPKEQGKLNEKCASKLYYNNMAICMLPLCHDACKSYDQWITRKKKQWDVL
                     STKFSSVKKTQKIGTENIATAYDILKQELNGFKEATFENEINKRDNLYNHLCPCVVEE
                     ARKNTQENVKNVGSGVESKAASSNPITEAVKSSSGEGKVQEDSAHKSVNKGEGKSSTN
                     EADPGSQSGAPASRSVDEKAGVPALSAGQGHDKVPPAEAAATESAVLHSADKTPNTVT
                     EENKEGTQMDGAAGGDGKAPGPTVSSDVPSVGGKDSGPSTSASHALAGENGEVHNGTD
                     TEPKEDGEKADPQKDIEVKGKQDTDDRSQGSLGPHTDERATLGETHMEKDTETAGGST
                     LTPEQNVSVASDNGNVPGSGNKQNEGATALSGAESLKSNESVHKTIDNTTHGLENKNG
                     GNEKDFQKHDFMNNDMLNDQASSDHTSSDQTSSDHTSSDQTSSDHTSSDHTSSDQTSS
                     DQTSSDQTIDTEGHHRDNVRNPEIKSSEDMSKGDFMRNSNSNELYSHNNLNNRKLNRD
                     QYEHRDVKATREKIILMSEVNKCNNRASVKYCNTIEDRMLSSTCSRERRKNLCCSISD
                     FCLNYFELYSYEFYNCMKKEFEDPSYECFTKGSSTGIVYFATGGAFLIILLLFASWNA
                     ASNDYEEEATFDEFVEYSDDIHRTPLMPNDIEHMQQFTPLDYS"
     intron          514..681
                     /number=1
                     /cons_splice=(5'site:yes,3'site:yes)
                     /gene="alpha"
                     /label=intron1
     exon            682..3643
                     /number=1
                     /function="erythrocyte binding domain"
                     /gene="alpha"
     intron          3644..4065
                     /number
                     /cons_splice=(5'site:yes,3'site:yes)
                     /gene="alpha"
                     /evidence=EXPERIMENTAL
     exon            3750..3828
                     /number=2
                     /function="transmembrane domain"
                     /gene="alpha"
     intron          3751..3827
                     /number=3
                     /cons_splice=(5'site:yes,3'site:yes)
                     /gene="alpha"
                     /evidence=EXPERIMENTAL
     exon            4066..4142
                     /number=3
                     /function="cytoplasmic domain"
                     /gene="alpha"
     intron          4143..4224
                     /number=4
                     /cons_splice=(5'site:yes,3'site:yes)
                     /gene="alpha"
                     /evidence=EXPERIMENTAL
BASE COUNT     1787 a    641 c    963 g   1354 t
ORIGIN
        1 agtgtaaagt ccttgttcta aaaaaagaat agtactacag gtgttgcata atgtgtacac
       61 gtgtaaatgc cttgttctaa taaaaaaaaa aagagtacta cagatgttgc attaatgtgt
      121 acgcgtaaag tgccttgttc taatgaaagg aaaaaaaaaa aaaaattaaa aagggggttt
      181 aaatgtcgta ataggaaatt gtaataaaga attacccaca gcagagtcac aacatttgat
      241 gtgttttaca ccccccatat atatgtaggt tcatataatt tagttctggg tacttttctt
      301 tttcgtcttc tttttatata catgcgttca aatttgttct gtgctttaca tgttcagtta
      361 tatgtgtaga acacctagct acataatttg gtatacgccc ccctgaatgt gctttgtata
      421 atataaccac gtatacactt atttttaaat acaatggaag gaaaaaaaaa acgtccttta
      481 ttttttctcc tagttttatt attgtcacac aaggtaatga aggaaaattt tttttaccag
      541 actaataaat tatcatgaag aaaaagaaaa aaaaaaaaaa aaaaaggggg acatttaatt
      601 atactagaag aagaggggaa caaaatgaag aaagctaaga atcgtgatta gtataatctg
      661 ttctctttct ttttctttta ggctaataat gtattatttg aacgaatgaa tggaatcctt
      721 ttactagaat gcgaaaatga atacgtgaaa aatgaaaatg gttataaatt agctacagga
      781 catcattata tggacaacga tcaaatagaa caatggttac aaggaactga tagaagcaga
      841 agagtgaaga ttgaggaaaa tgtaaaatat aaatataacg tagaggaact aaatacaaaa
      901 tatgaacaaa tgaaagcccc aagaattaac cgcattttga aggaatccac ttacgaggcg
      961 caaaatgttg cagacaacaa ttatatagat gataaagcaa atggggaaca taaaactgat
     1021 aataaaacta ataaggggga aggtgcaagg aatatggtca tgttggatta tgatatatct
     1081 ggcagtggcc agccagatgg aatcattgat aatgttgttg aattgggaac tgaggatgag
     1141 ggaaattttc ttgaaaattc ctcaaaaggc ggtgaccatc cttatcgtat gaatcgaaag
     1201 gaaagaatgt ctaatggagt tattaatcaa acttttcttc aaaacaatgt aatggacaag
     1261 tgtaatgata agagaaaacg cggggaaaga gattgggact gtccagctga gaaggatatt
     1321 tgtatatcag tgcgaagata tcaattatgt atgaagggac ttacgaattt agtaaataat
     1381 acaaggacac attctcataa tgatataaca tttctgaaat taaatttgaa aaggaaactt
     1441 atgtatgatg ctgctgtgga gggcgattta ttattaaaga agaataacta ccaatataac
     1501 aaagaattct gtaaggatat aagatggggg ttgggggatt ttggagacat aattatggga
     1561 actaatatgg aaggtattgg gtattcccaa gtagtggaaa ataatttgcg ccaagtcttt
     1621 ggaactgatg aaaaggccaa gcaggatcgt aaacaatggt ggaatgaatc taaggaacat
     1681 atatggagag caatgatgtt ctcaattagg agcagattaa aggagaaatt tgtgtggatt
     1741 tgtaaaaaag atgttacgtt aaaagtagaa ccccagatat acagatggat tcgagaatgg
     1801 ggaagagatt atatgtcaaa attacccaaa gaacagggga aactaaatga aaaatgtgct
     1861 agtaaattat actataataa tatggcaata tgtatgttgc ctctgtgcca tgatgcctgt
     1921 aaatcatatg atcaatggat aacaaggaaa aaaaaacaat gggatgtttt gtcaacaaaa
     1981 ttttcaagtg taaagaagac acaaaaaatc gggacggaaa atatcgcaac agcttatgat
     2041 atactaaaac aggaattaaa tggatttaaa gaggcgactt ttgagaatga aattaacaaa
     2101 cgtgataatt tatataatca tttatgccct tgtgtcgtgg aggaggctag aaagaatacc
     2161 caggaaaatg tgaaaaatgt aggaagtggt gttgaatcta aggcagccag ttctaatccg
     2221 ataactgaag ctgtaaaaag tagtagcggg gaggggaagg ttcaggagga ttctgcacac
     2281 aaaagtgtta acaaaggtga aggtaagtct agcacaaatg aagctgatcc aggttctcaa
     2341 tcaggtgctc ctgcttctcg tagtgtagat gagaaggcag gtgttcctgc tctatcagct
     2401 ggtcaaggtc atgataaagt tccccctgct gaagctgctg ctacagaatc agctgttctg
     2461 cattcagcag acaaaactcc aaatacagta acagaagaaa ataaggaagg aacccagatg
     2521 gatggtgctg cgggtggaga tggtaaggct cctggtccaa ctgtttcttc cgatgttcct
     2581 agtgttgggg gtaaggatag tggtcccagt acctctgcgt cccatgctct cgctggggaa
     2641 aatggtgaag ttcataatgg cactgatact gaacctaagg aagatggtga gaaggctgat
     2701 ccccagaaag atatagaagt aaagggtaag caagatacag atgataggtc acaggggtca
     2761 cttgggccac atactgatga aagagcaact ttgggggaaa ctcatatgga gaaggataca
     2821 gaaaccgcag gaggtagtac tctcactccc gaacagaatg ttagtgttgc ttctgataat
     2881 ggtaatgttc ctggatctgg caataagcaa aatgagggtg caactgcgtt gagtggagct
     2941 gaaagtttga aatcaaacga aagtgtacat aaaactattg ataatacaac tcacggttta
     3001 gaaaataaaa atggaggaaa cgaaaaggat tttcagaagc acgattttat gaataatgac
     3061 atgttgaatg atcaagcaag ttctgatcac acaagttcag accaaacaag ttctgatcat
     3121 acaagttcag atcaaacaag ttcagatcac acaagttctg atcacacaag ttctgatcaa
     3181 acaagttcag atcaaacaag ttctgatcaa actatagata cagaaggaca tcacagggat
     3241 aatgtcagaa atcctgaaat aaagagttcg gaagatatga gtaaagggga ttttatgaga
     3301 aattcaaata gtaacgaatt atatagtcat aataatttga ataatcgtaa attaaataga
     3361 gaccaatacg aacacagaga tgtcaaagca acaagggaaa aaattatcct tatgtctgaa
     3421 gtaaacaaat gcaataatag ggcatccgta aaatactgta acactataga agacagaatg
     3481 ttatcgagca cttgttcaag ggagaggagg aaaaatttat gttgttcaat atcggatttt
     3541 tgtttgaatt attttgagct ctattcttat gaattttata attgcatgaa aaaggaattt
     3601 gaagatccat catacgagtg ctttacaaaa ggaagctcca caggtataca ggaaaagatg
     3661 atcaatagac aaagatgtta cgtacattga aaataaattc attttcggaa tgttataaat
     3721 ttttttgtaa tcaatattct tttttgcagg catagtttat tttgccacgg ggggagcgtt
     3781 tctgataata ctgctactat ttgcctcatg gaatgcagcc tcgaatgagt aaccaaaaaa
     3841 taatataaaa attagaataa gaataagaat tagagtggaa gctagattaa caattaaaat
     3901 aaaaaataaa atagaaaatg ctgttaatgc acaattaatt ctatatattc catgtgtgca
     3961 attttaagga gagtaaaaat gtgaaatcat tatatgcatg cacgtataca tatatagaca
     4021 tatatgtacc aatatataat aatgcacact tccttgttcg tacagttatg aagaagaagc
     4081 tacatttgat gagttcgtgg aatattctga tgatattcac agaacccctc taatgcctaa
     4141 cggtaattca aatttcaaaa gcaaaatttc atttatgaag aaatattaca ccattctgca
     4201 ttattccttt tatttcttct ttagatattg agcacatgca acaatttaca cccctggatt
     4261 attcataatg ctacttgggt aagtaaggag aattgtgata gtacagctga tggaattttt
     4321 gtcattttgc ttaatgcagt aaatgagaag tatatgaacg gttcggcaaa gaatgattta
     4381 acagcactat gacaattact tgtactatgc gtctttattt aaatatctaa attagaatta
     4441 atttttttgt atttgtaata agtcaataca agtgtatttc cttttattgg cttgtaggat
     4501 gtgtagtcct ttagccaata gtagaaaaat tcgtttaggc gaattgtata attttccttt
     4561 tttttttatt tgaacttttt atttttctta tattgacaaa aaaaaaaaaa aaaaaaaaaa
     4621 taccatttca ataatttgaa gacatattga tggaagaaaa aaagaaattg tcccaaaaga
     4681 gaaaaatgtg ccattaattt gtcaaaaaaa gaaaatgtcc ctaataatta aatttgctac
     4741 acggc
//