From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Bacillus anthracis 590 macrolide-lincosamide-streptogramin B
Date: 3 Jan 93 22:11:39 GMT
Distribution: bionet
Checksum: 36449 4

LOCUS       BACMLSBRES   1324 bp ds-DNA             BCT       23-DEC-1992
DEFINITION  Bacillus anthracis 590 macrolide-lincosamide-streptogramin B
            resistance gene, complete cds.
ACCESSION   L08389
KEYWORDS    lincosamide resistance; macrolide resistance;
            rRNA methyltransferase; streptogramin B resistance.
SOURCE      Bacillus anthracis (library: E.coli NM 522) DNA.
  ORGANISM  Bacillus anthracis
            Prokaryotae; Firmicutes; Endospore-forming rods and cocci;
            Bacillaceae.
REFERENCE   1  (bases 1 to 1324)
  AUTHORS   Kim,H.-S., Choi,E.-C.C. and Kim,B.-K.
  TITLE     A macrolide-lincosamide-streptogramin B resistance determinant form
            Bacillus anthracis 590: Cloning and expression of ermJ
  JOURNAL   J. Gen. Microbiol. (1992) In press
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     -35_signal      1..6
                     /gene="ermJ"
     -10_signal      25..30
                     /gene="ermJ"
     mRNA            34..1287
                     /gene="ermJ"
     protein_bind    53..59
                     /bound_moiety="SD-1"
     protein_bind    375..384
                     /bound_moiety="SD-2"
     CDS             70..114
                     /gene="ermJ"
                     /product="leader peptidse"
                     /codon_start=1
                     /translation="MTHSMRLRFPTLNQ"
     CDS             391..1254
                     /gene="ermJ"
                     /product="rRNA methyltransferase"
                     /codon_start=1
                     /translation="MKKKNHKYRGKKLNRGESPNFSGQHLMHNKKLIEEIVDRANISI
                     DDTVLELGAGKGALTTVLSQKAGKVLAVENDSKFVDILTRKTAQHSNTKIIHQDIMKI
                     HLPKEKFVVVSNIPYAITTPIMKMLLNNPASGFQKGIIVMEKGAAKRFTSKFIKNSYV
                     LAWRMWFDIGIVREISKEHFSPPPKVDSAMVRITRKKDAPLSHKHYIAFRGLAEYALK
                     EPNIPLCVRLRGIFTPRQMKHLRKSLKINNEKTVGTLTENQWAVIFNTMTQYVMHHKW
                     PRANKRKPGEI"
BASE COUNT      446 a    246 c    264 g    368 t
ORIGIN      
        1 gttgacgttt tccaaagaat gccctacaat gagatcgtaa ctttaacttt tcaggaggat
       61 tattaaaaaa tgacacactc aatgagactg cgtttcccaa ctttgaacca gtaattaaat
      121 acgttcaaag gctctgtttg tgtatgcaga gtaaacggga tcagtctgtc cttttttaat
      181 aatcttcaat ttgcatataa aatttgtaat ttgtagaaag gcggatcgtt ctgcctttct
      241 tttttgttta atcctttttt tctaacggct gagaaaggct tatttggtta tggaaatacc
      301 gcggcttcat ccttaaagga tggctcttcc ctttactctg aatcacaggc agaccgcctg
      361 tgatttttta tgatgagagg aagaggaaac atgaagaaaa aaaatcataa gtacagagga
      421 aaaaagttaa accgcgggga atctccgaat ttttccggac agcatttgat gcataataaa
      481 aaattaattg aagaaattgt ggatcgggca aatattagca tagacgatac ggttttagag
      541 ttaggagcgg gaaaaggggc tttgacaact gtgctaagtc aaaaagccgg taaggtattg
      601 gcagtggaaa acgattctaa attcgttgat atactcacac gtaaaacagc acagcattca
      661 aatacgaaaa ttattcatca agatatcatg aagattcatt taccaaaaga aaagtttgtg
      721 gtggtctcta atattcccta tgccatcaca actcccatca tgaaaatgct cttgaacaat
      781 cctgcaagcg gatttcaaaa agggatcatc gtaatggaaa aaggggctgc taaacgtttc
      841 acatcaaaat tcattaaaaa ttcctatgtt ttagcttgga gaatgtggtt tgatattggc
      901 attgtcagag aaatatcgaa agagcatttt tctccccctc caaaagtgga ctcggcaatg
      961 gtcagaataa cacgaaaaaa agacgcgcct ctatcacata aacattatat tgcgtttcgg
     1021 ggacttgccg aatacgcgct aaaggagccg aatatccctc tctgtgttcg tttacgcgga
     1081 atttttaccc cgcgtcaaat gaaacactta agaaaaagtc taaaaatcaa caatgaaaaa
     1141 accgttggaa cgctcaccga aaaccaatgg gcggttattt ttaacacgat gactcaatat
     1201 gtaatgcatc acaaatggcc aagagcaaat aagcgaaaac ccggagaaat ataaagaaaa
     1261 agctgctgac ttcccgtcag cagctttagc tattttctgg agggattcag atgtccctgt
     1321 caac
//