HDTenEightyP/NetNews huggingface dataset:
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: B.campestris gene for 2S storage protein
Date: 15 Aug 92 10:01:15 GMT
Distribution: bionet
Checksum: 02492     2

LOCUS       BNA2SSP       537 bp ds-DNA             PLN       15-AUG-1992
DEFINITION  B.campestris gene for 2S storage protein
ACCESSION   X65037
KEYWORDS    storage protein.
SOURCE      Brassica campestris DNA.
  ORGANISM  Brassica campestris
            Eukaryota; Plantae; Embryobionta; Magnoliophyta; Magnoliopsida;
            Dilleniidae; Capparales; Brassicaceae.
REFERENCE   1  (bases 1 to 537)
  AUTHORS   Das Gupta,J., Das Gupta,S. and Mandal,R.K.
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
COMMENT     *source: strain=B9; *source: stage=germinated seedling; *source:
            clone=pBc2SC;
            PCR amplified genomic DNA
            From EMBL    entry BC2SSP;  dated 06-AUG-1992.
FEATURES             Location/Qualifiers
     CDS             1..537
                     /product="2S storage protein"
                     /codon_start=1
                     /translation="MANKLFLVSATLALFFLLTNASVYRTGSEFDEHDATNPAGPFRI
                     PKCRKEFQQAQHLKACQQWLHKQAMQSGSGPSWTLDGEFDFEEDMENTQGPQQEPPLL
                     QQCCNELHQEEPLCVCPTLKGASKAVKQQVRQQGQQQQMQQVISRIYQTSTHLPRVCN
                     IRQVSICPFQKTMPGPSY"
BASE COUNT      150 a    157 c    121 g    109 t
ORIGIN
        1 atggcgaaca agctcttcct cgtctcggca actctcgcct tgttcttcct tctcaccaat
       61 gcctccgtct acaggaccgg ttctgagttc gacgaacatg atgccacaaa tccagccggc
      121 ccatttagga ttccaaaatg taggaaggag tttcagcaag cacaacacct gaaagcttgc
      181 caacaatggc tccacaagca ggcaatgcag tccggtagtg gtccaagctg gaccctcgat
      241 ggtgagtttg attttgaaga agacatggag aacacacagg gcccacagca ggagccaccg
      301 ctactccagc agtgctgcaa cgagctccac caggaagagc cactttgcgt ttgcccaacc
      361 ttgaaaggag catccaaagc cgttaaacaa caggttcgac aacagggaca acaacagcag
      421 atgcagcaag taattagccg tatctaccag acctctacgc acttacctag agtttgcaac
      481 atcaggcaag ttagcatttg tcccttccag aagaccatgc ctgggccctc ctactag
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human chromosome 4 sequence-tagged site STS4-116
Date: 21 Aug 92 10:06:43 GMT
Distribution: bionet
Checksum: 21217     3

LOCUS       HUM4STS116    373 bp ds-DNA             PRI       21-AUG-1992
DEFINITION  Human chromosome 4 sequence-tagged site STS4-116
ACCESSION   M95277
KEYWORDS    STS; human chromosome 4; sequence tagged site.
SOURCE      Homo sapiens, clone C4-78 from Los Alamos National Laboratory
            chromosome 4 cosmid library, plate 4-1-1R, pos 12B; vector SuperCos
            1 (Stratagene)
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 373)
  AUTHORS   Goold,R.D., diSibio,G., Dugaiczyk,A., Smith,K.A., Xu,H.,
            Magrane,G., Lang,D.B., Cox,D.R., Masters,S.B. and Myers,R.M.
  TITLE     Sequence-Tagged Sites for Human Chromosome 4
  JOURNAL   Unpublished (1992) see COMMENT for author address
  STANDARD  full automatic
COMMENT     PCR components: 25 ng of human genomic DNA, 10 pmol of each
            oligonucleotide, 200 micro-M dNTPs, 0.25 U Taq  polymerase (Cetus)
            in 10 micro-l of 50 mM KCl-20 mM  Tris-HCl, pH 8.3 (at room temp),
            2.5 mM MgCl-2.  Initial denaturation at 94degC for 1.5 min, then 30
            cycles of  94degC for 15 sec, 62degC for 23 sec, and 72degC for 30
            sec, followed by a final extension at 72degC for 3.5 min, using a
            Perkin-Elmer 9600 thermocycler. PCR-amplified product size 238 bp.
            Sequence submitted by:
            Human Genome Mapping Center
            Box 0925
            University of California San Francisco
            San Francisco, CA 94143-0925  USA
            Phone: (415) 502-1612   Fax: (415) 476-8391
            e-mail: hgmcprobes@cgl.ucsf.edu
FEATURES             Location/Qualifiers
     misc_feature    56..78
                     /label=Primer1
     misc_feature    complement(269..293)
                     /label=Primer2
BASE COUNT      108 a     78 c     54 g    119 t     14 others
ORIGIN
        1 nnctnactgc aggcccctgg gcacncttgt ttaaaantgt ngattgtncc tgagcccctc
       61 tccagattca ccgaatcaga gattttnggc ctctagacca gaaatctaca ttcagacaac
      121 ctccttagct gcacccaagt ttaagaatgc aaaacgtaaa aatatccatt taaaaatgta
      181 ttttggaacg tttctgtctg agctctttta tgcatgcttt ttcagctaga tcctgtgtgt
      241 atcacctaac attgcataaa aattattatc acacaacttt ggcacatatt tgtaaacatc
      301 atttttaatg gctgtataat ataacctgna aagatnctct aatnnacttg tcatgttntt
      361 caacatttag gan
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human chromosome 4 sequence-tagged site STS4-487
Date: 21 Aug 92 10:10:50 GMT
Distribution: bionet
Checksum: 09483     3

LOCUS       HUM4STS487    406 bp ds-DNA             PRI       21-AUG-1992
DEFINITION  Human chromosome 4 sequence-tagged site STS4-487
ACCESSION   L00885
KEYWORDS    STS; human chromosome 4; sequence tagged site.
SOURCE      Homo sapiens, clone p4-559 from a chromosome 4 plasmid library;
            vector pBluescript II KS + (Stratagene)
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 406)
  AUTHORS   Goold,R.D., diSibio,G., Dugaiczyk,A., Smith,K.A., Xu,H.,
            Magrane,G., Lang,D.B., Cox,D.R., Masters,S.B. and Myers,R.M.
  TITLE     Sequence-Tagged Sites for Human Chromosome 4
  JOURNAL   Unpublished (1992) see COMMENT for author address
  STANDARD  full automatic
COMMENT     PCR components: 25 ng of human genomic DNA, 10 pmol of each
            oligonucleotide, 200 micro-M dNTPs, 0.25 U Taq  polymerase (Cetus)
            in 10 micro-l of 50 mM KCl-20 mM  Tris-HCl, pH 8.3 (at room temp),
            2.5 mM MgCl-2.  Initial denaturation at 94degC for 1.5 min, then 30
            cycles of  94degC for 15 sec, 62degC for 23 sec, and 72degC for 30
            sec, followed by a final extension at 72degC for 3.5 min, using a
            Perkin-Elmer 9600 thermocycler. PCR-amplified product size 238 bp.
            Sequence submitted by:
            Human Genome Mapping Center
            Box 0925
            University of California San Francisco
            San Francisco, CA 94143-0925  USA
            Phone: (415) 502-1612   Fax: (415) 476-8391
            e-mail: hgmcprobes@cgl.ucsf.edu
FEATURES             Location/Qualifiers
     misc_feature    22..44
                     /label=Primer1
     misc_feature    complement(237..259)
                     /label=Primer2
BASE COUNT       89 a     99 c    122 g     95 t      1 others
ORIGIN
        1 gatctgttac ctggcctgac ttttcctccc ccttgtctac taaccatgtc cagtctggat
       61 gggaggtcac agggtcacct ttaactccaa ggggaagttg tcaggccaag aggctcagct
      121 gttggattcc aggttggaga gctgagggtt ccgatgaaag gcacaaagac cgagctgcac
      181 actgcgagca tcagtacttc ctttcctacc ctccctgtga gaaatgcctg agctgtgggt
      241 caagaagagg agaattggag agcaccaggg ggtcaggtgg gtgggaggag ccttcacatt
      301 ccactttgag ttccatggtg gccccgatgg aagctgccct catgtttggg acccaggagt
      361 gcaagagatc agatttaggg cgggcatngt ggctctcacc tgtaat
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Fellomyces polyborus, 18S rRNA, partial sequence, positions
Date: 13 Aug 92 10:00:21 GMT
Distribution: bionet
Checksum: 42411     2

LOCUS       FEL18SFE      170 bp ss-rRNA            RNA       13-AUG-1992
DEFINITION  Fellomyces polyborus, 18S rRNA, partial sequence, positions
            1451-1618 (in S. cerevisiae).
ACCESSION   D00901
KEYWORDS    18S ribosomal RNA.
SOURCE      Fellomyces polyborus (library: IFO 10120) rRNA.
  ORGANISM  Fellomyces polyborus
            Eukaryota; Fungi; Eumycota; Deuteromycotina; Ascomycetes.
REFERENCE   1  (bases 1 to 170)
  AUTHORS   Yamada,Y., Kawasaki,H., Nakase,T. and Banno,I.
  TITLE     The phylogenetic relationship of the conidium-forming anamorphic
            yeast genera Sterigmatomyces, Kurtzmanomyces, Tsuchiyaea and
            Fellomyces, and the teleomorphic yeast genus Sterigmatosporidium on
            the basis of the partial sequences of 18S and 26S riboso
  JOURNAL   Agric. Biol. Chem. 53, 2993-3001 (1989)
  STANDARD  full staff_entry
COMMENT     .
FEATURES             Location/Qualifiers
     rRNA            <1..>170
                     /note="18S rRNA"
BASE COUNT       40 a     44 c     47 g     39 t
ORIGIN
        1 gggccgcacg cgcgctacac tgactgagcc agcgagtaca tcaccttggc cgagaggtct
       61 gggtaatctt gtgaaactca gtcgtgctgg ggatagagca ttgcaattat tgctcttcaa
      121 cgaggaatac ctagtaagcg tgagtcacca actcgcgttg attacgtccc
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human chromosome 4 sequence-tagged site STS4-161
Date: 21 Aug 92 10:07:11 GMT
Distribution: bionet
Checksum: 16311     3

LOCUS       HUM4STS161    435 bp ds-DNA             PRI       21-AUG-1992
DEFINITION  Human chromosome 4 sequence-tagged site STS4-161
ACCESSION   M95263
KEYWORDS    STS; human chromosome 4; sequence tagged site.
SOURCE      Homo sapiens, clone C4-177 from Los Alamos National Laboratory
            chromosome 4 cosmid library, plate 4-1-2R, pos3C; vector SuperCos 1
            (Stratagene)
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 435)
  AUTHORS   Goold,R.D., diSibio,G., Dugaiczyk,A., Smith,K.A., Xu,H.,
            Magrane,G., Lang,D.B., Cox,D.R., Masters,S.B. and Myers,R.M.
  TITLE     Sequence-Tagged Sites for Human Chromosome 4
  JOURNAL   Unpublished (1992) see COMMENT for author address
  STANDARD  full automatic
COMMENT     PCR components: 25 ng of human genomic DNA, 10 pmol of each
            oligonucleotide, 200 micro-M dNTPs, 0.25 U Taq  polymerase (Cetus)
            in 10 micro-l of 50 mM KCl-20 mM  Tris-HCl, pH 8.3 (at room temp),
            2.5 mM MgCl-2.  Initial denaturation at 94degC for 1.5 min, then 30
            cycles of  94degC for 15 sec, 62degC for 23 sec, and 72degC for 30
            sec, followed by a final extension at 72degC for 3.5 min, using a
            Perkin-Elmer 9600 thermocycler. PCR-amplified product size 279 bp.
            Sequence submitted by:
            Human Genome Mapping Center
            Box 0925
            University of California San Francisco
            San Francisco, CA 94143-0925  USA
            Phone: (415) 502-1612   Fax: (415) 476-8391
            e-mail: hgmcprobes@cgl.ucsf.edu
FEATURES             Location/Qualifiers
     misc_feature    62..84
                     /label=Primer1
     misc_feature    complement(316..340)
                     /label=Primer2
BASE COUNT      128 a     64 c     75 g    162 t      6 others
ORIGIN
        1 gnncctgtct caaccataac gcatttgcct tctcaagatt ctnctggaaa gagtcaaggn
       61 agccacagac acaatgaagc atgtnaagca gcagcagaaa taataaaagc tatgtttcac
      121 caaatgttga atatgtacaa agcacagaac tctgcacttt acattttgtt ttgtcttatt
      181 caggcaacta aataacctat gaggtaaaga ctattatatt cccatcttag gtgagtacaa
      241 aagtaattat ggtttttgca ttgttggaat ttgctgtttg atattaggat actttcttaa
      301 atgtggttat gttatacatc atttcaatgg gcatttcttg cttttgttgt tgtttttttt
      361 tttgctaatg ntttattact tgctgtttat tttatgttta ttttagacta tggaaatgat
      421 gttagacaaa agaca
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human chromosome 4 sequence-tagged site STS4-345
Date: 21 Aug 92 10:09:11 GMT
Distribution: bionet
Checksum: 25584     3

LOCUS       HUM4STS345    442 bp ds-DNA             PRI       21-AUG-1992
DEFINITION  Human chromosome 4 sequence-tagged site STS4-345
ACCESSION   L00805
KEYWORDS    STS; human chromosome 4; sequence tagged site.
SOURCE      Homo sapiens, clone p4-254 from a chromosome 4 plasmid library;
            vector pBluescript II KS + (Stratagene)
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 442)
  AUTHORS   Goold,R.D., diSibio,G., Dugaiczyk,A., Smith,K.A., Xu,H.,
            Magrane,G., Lang,D.B., Cox,D.R., Masters,S.B. and Myers,R.M.
  TITLE     Sequence-Tagged Sites for Human Chromosome 4
  JOURNAL   Unpublished (1992) see COMMENT for author address
  STANDARD  full automatic
COMMENT     PCR components: 25 ng of human genomic DNA, 10 pmol of each
            oligonucleotide, 200 micro-M dNTPs, 0.25 U Taq  polymerase (Cetus)
            in 10 micro-l of 50 mM KCl-20 mM  Tris-HCl, pH 8.3 (at room temp),
            2.5 mM MgCl-2.  Initial denaturation at 94degC for 1.5 min, then 30
            cycles of  94degC for 15 sec, 62degC for 23 sec, and 72degC for 30
            sec, followed by a final extension at 72degC for 3.5 min, using a
            Perkin-Elmer 9600 thermocycler. PCR-amplified product size 234 bp.
            Sequence submitted by:
            Human Genome Mapping Center
            Box 0925
            University of California San Francisco
            San Francisco, CA 94143-0925  USA
            Phone: (415) 502-1612   Fax: (415) 476-8391
            e-mail: hgmcprobes@cgl.ucsf.edu
FEATURES             Location/Qualifiers
     misc_feature    15..38
                     /label=Primer1
     misc_feature    complement(225..248)
                     /label=Primer2
BASE COUNT      107 a     95 c    116 g    121 t      3 others
ORIGIN
        1 gatctgctgg tggtcgtggt tcaactttct aagccactgc cttgggcttc agtttcccca
       61 gctggaggat aaagtgtttg gaccagataa cctccgatta ctggctggct accctgctgt
      121 acccttgctg agctctgccc agggtgacct gagctcttgt gggtgcatgt gtgtgtccca
      181 gaagggctgg gctccagctg gtcagagaga gaacacgcgg agccatgtca ggaaagtgga
      241 cacaatacca tatggaagac accgctgctc agcctggatg ggtctagcta gatgtcattt
      301 ttaaaaacat tcctttttat gattttaata gtaatgcaca tgcattgtgt ggatattaaa
      361 tgacgtgata caacctcgaa ataaaccagg aggtaaaagt gtgttaacac tgggananaa
      421 gccttttagt ttccntctgg tg
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Gorilla gorilla MHC DQB2*01 gene, partial cds, clone GoDQB2*01.
Date: 12 Aug 92 10:00:37 GMT
Distribution: bionet
Checksum: 35227     2

LOCUS       GORMHDQBA     246 bp ds-DNA             PRI       12-AUG-1992
DEFINITION  Gorilla gorilla MHC DQB2*01 gene, partial cds, clone GoDQB2*01.
ACCESSION   M81268
KEYWORDS    major histocompatibility complex.
SOURCE      Gorilla gorilla (individual_isolate Wilma) blood DNA.
  ORGANISM  Gorilla gorilla
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Pongidae.
REFERENCE   1  (bases 1 to 246)
  AUTHORS   Bontrop,R.E., Jonker,M., van Weeren,P., Kenter,M. and Otting,N.
  TITLE     MHC-DQB repertoire variation in hominoid and old world primate
            species
  JOURNAL   J. Immunol. 149, 461-470 (1992)
  STANDARD  full staff_entry
FEATURES             Location/Qualifiers
     CDS             <1..>246
                     /gene="MHC DQ-beta 2"
                     /label=exon 2
                     /codon_start=1
                     /translation="VQFKGMCYFTNGTERVRGVARHIYNREEYGRFDSDVGEFQAVTE
                     LGRXSIEDWNNYKDFLEQERAAVDKVCRHNYEAELRTT"
BASE COUNT       57 a     60 c     89 g     37 t      3 others
ORIGIN
        1 gtccagttta agggcatgtg ctacttcacc aacgggacag agcgcgtgcg gggtgtggcc
       61 agacacatct ataaccgcga ggagtacggg cgcttcgaca gcgacgttgg ggagttccag
      121 gcggtgaccg agctggggcg gnnnagcatc gaggactgga acaactataa ggacttcttg
      181 gagcaggagc gggccgcggt ggacaaggtg tgcagacaca actacgaggc ggagctacgc
      241 acgacc
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: SCET-VH
Date: 13 Aug 92 10:00:57 GMT
Distribution: bionet
Checksum: 55812     3

LOCUS       MUSD12736     358 bp ds-DNA             ROD       13-AUG-1992
DEFINITION  SCET-VH
ACCESSION   D12736
KEYWORDS    .
SOURCE      Mus musculus (strain BALB/c) DNA.
  ORGANISM  Mus musculus
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Rodentia; Myomorpha; Muridae; Murinae.
REFERENCE   1  (bases 1 to 358)
  AUTHORS   Sawada,J., Mizusawa,S., Terao,T., Naito,M. and Kurosawa,Y.
  TITLE     Molecular characterization of monoclonal anti-steroid antibodies:
            primary structures of the variable regions of seven antibodies
            specific for 17alpha-hydroxyprogesterone or 11-deoxycortisol and
            their pH-reactivity profiles
  JOURNAL   Mol. Immunol. 28, 1063-1072 (1991)
  STANDARD  full automatic
COMMENT     Submitted (27-JUL-1992) to DDBJ by:
            Jun-ichi Sawada
            Dept. of Biochem. and Immunochem.
            National Institute of Hygienic Sciences
            1-18-1 Kamiyoga
            Setagaya, Tokyo 158
            Japan
            Phone:  03-3700-1141 x240
            Fax:    03-3707-6950
FEATURES             Location/Qualifiers
     CDS             <1..>358
                     /product="'Ig gamma chain precursor'"
                     /codon_start=1
                     /translation="QIQLVQSGPELKKPGETVKISCKASGYTFTNYGMNWVKQAPGKG
                     LEWMDWINTYTGEPTYADDFKGRFAFSLETSASTAYLQINDLKNEDTATYFCARGNVR
                     VYAMDYWGQGTSVTVSS"
     mat_peptide     1..>358
                     /note="'VDJ region (1-358)'"
                     /product="'Ig gamma chain'"
                     /codon_start=1
BASE COUNT       99 a     82 c     94 g     83 t
ORIGIN
        1 cagatccagt tggtgcagtc tggacctgag ttgaagaagc ctggagagac agtcaagatc
       61 tcctgcaagg cttctgggta taccttcaca aactatggaa tgaactgggt gaagcaggct
      121 ccaggaaagg gtttagagtg gatggactgg ataaacacct acactggaga gccaacatat
      181 gctgatgact tcaagggacg atttgccttc tctttggaaa cctctgccag cactgcctat
      241 ttgcagatca acgacctcaa aaatgaggac acggctacat atttctgtgc aagaggtaac
      301 gtcagggtct atgctatgga ctactggggt caaggaacct cagtcaccgt ctcctcag
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Lentinus ponderosus ribosomal RNA (15LR0R) large subunit, partial.
Date: 19 Aug 92 10:01:41 GMT
Distribution: bionet
Checksum: 36027     2

LOCUS       LENRRLSAM     234 bp ss-rRNA            RNA       19-AUG-1992
DEFINITION  Lentinus ponderosus ribosomal RNA (15LR0R) large subunit, partial.
ACCESSION   M98645
KEYWORDS    large subunit ribosomal RNA.
SOURCE      Lentinus ponderosus (library: Duke culture collection 606) cDNA to
            rRNA.
  ORGANISM  Lentinus ponderosus
            Eukaryota; Fungi; Eumycota; Basidiomycotina; Hymenomycetes;
            Agaricales; Tricholomataceae.
REFERENCE   1  (bases 1 to 234)
  AUTHORS   Hibbett,D.S. and Vilgalys,R.
  TITLE     Phylogenetic relationships of the basidiomycete genus Lentinus
            inferred from molecular and morphological characters
  JOURNAL   Unpublished (1992)
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     rRNA            <1..>234
                     /note="Approximately aligns to region between positions 70
                     to 306in Saccharomyces cerevisiae large subunit nuclear
                     rRNA."
                     /product="large subunit ribosomal RNA"
                     /citation=[1]
BASE COUNT       53 a     48 c     70 g     63 t
ORIGIN
        1 attcccctag taactgcgag tgaagcggga agagctcaaa tttaaaatct ggcggtcctt
       61 tggccgtccg agttgtattc tggagaagtg ctttccgcgc tggaccgtgt ataagtctct
      121 tggaacagag cgtcgtagag ggtgagaatc ccgtctttga cacggactac cagtgctttg
      181 tgatgcgctc tcaaagagtc gagttgtttg ggaatgcagc tcaaaatggg tggt
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human chromosome 4 sequence-tagged site STS4-392
Date: 21 Aug 92 10:09:48 GMT
Distribution: bionet
Checksum: 25739     3

LOCUS       HUM4STS392    414 bp ds-DNA             PRI       21-AUG-1992
DEFINITION  Human chromosome 4 sequence-tagged site STS4-392
ACCESSION   L00835
KEYWORDS    STS; human chromosome 4; sequence tagged site.
SOURCE      Homo sapiens, clone p4-405 from a chromosome 4 plasmid library;
            vector pBluescript II KS + (Stratagene)
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 414)
  AUTHORS   Goold,R.D., diSibio,G., Dugaiczyk,A., Smith,K.A., Xu,H.,
            Magrane,G., Lang,D.B., Cox,D.R., Masters,S.B. and Myers,R.M.
  TITLE     Sequence-Tagged Sites for Human Chromosome 4
  JOURNAL   Unpublished (1992) see COMMENT for author address
  STANDARD  full automatic
COMMENT     PCR components: 25 ng of human genomic DNA, 10 pmol of each
            oligonucleotide, 200 micro-M dNTPs, 0.25 U Taq  polymerase (Cetus)
            in 10 micro-l of 50 mM KCl-20 mM  Tris-HCl, pH 8.3 (at room temp),
            2.5 mM MgCl-2.  Initial denaturation at 94degC for 1.5 min, then 30
            cycles of  94degC for 15 sec, 62degC for 23 sec, and 72degC for 30
            sec, followed by a final extension at 72degC for 3.5 min, using a
            Perkin-Elmer 9600 thermocycler. PCR-amplified product size 241 bp.
            STS sequence contains a simple sequence tandem repeat (CA).
            Sequence submitted by:
            Human Genome Mapping Center
            Box 0925
            University of California San Francisco
            San Francisco, CA 94143-0925  USA
            Phone: (415) 502-1612   Fax: (415) 476-8391
            e-mail: hgmcprobes@cgl.ucsf.edu
FEATURES             Location/Qualifiers
     misc_feature    20..44
                     /label=Primer1
     misc_feature    complement(236..260)
                     /label=Primer2
BASE COUNT      158 a     68 c     60 g    119 t      9 others
ORIGIN
        1 gatcctatag attcatcttg ctcacttaca tttagaaagc gattcttggg tcaagatgta
       61 gtcttaaggt ctattaatac ttcnatatgc acatttnggn aacagtttta agaaataaat
      121 gcaacattta taaataataa ataataaata tgctaagaga aaaagtataa gatattctgc
      181 acaaagcatc gtaaacagtc atgatgcttt ttattnttac ggaaaatgac aganagttat
      241 aatgaaggaa cacgcacata cacacacaca cacacacaca cacttagata aataagcacc
      301 acataaaaag cttaaaagtn atagatttca gaaactgaca gtttnatcca cctttcacta
      361 gtgttacaaa tatatatgta acctgtntgg ttatntcact tcggggcgta gtaa
//