From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Synthetic rpsL gene for altered ribosomal protein S12.
Date: 31 Dec 92 01:41:03 GMT
Distribution: bionet
Checksum: 35231 5

LOCUS       SYNRPSLA     2405 bp ds-DNA             SYN       17-DEC-1992
DEFINITION  Synthetic rpsL gene for altered ribosomal protein S12.
ACCESSION   D10669
KEYWORDS    altered ribosomal protein S12.
SOURCE      Cloning vector DNA, clone pHSG673.
  ORGANISM  Cloning vector
            Artificial sequences; Cloning vehicles.
REFERENCE   1  (bases 1 to 2405)
  AUTHORS   Minowa,M. and Hashimoto-Gotoh,T.
  TITLE     Further improvement of Sms-enforcement cloning vectors with
            synthetic rpsL gene: Characterization of spontaneous elimination of
            streptomycin sensitivity gene on high copy number plasmids
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
COMMENT     Submitted (06-May-1992) to DDBJ by: Tamotsu Hashimoto-Gotoh
            Institute for Geriatrics and Neurological Diseases Dept. of
            Biochemistry and Molecular Genetics Kyoto Prefectural University of
            Medicine kawara-machi-Hirokoji Kajii-cho 465, Kamigyou-ku Kyoto 602
            Japan Phone:  075-251-5797 or 5850 Fax:    075-211-7093.
FEATURES             Location/Qualifiers
     misc_feature    1..375
                     /note="This feature applies to a gene which lacks a coding
                     region feature. "
                     /gene="rpsL"
     CDS             1..375
                     /product="altered ribosomal protein S12"
                     /gene="rpsL"
                     /codon_start=1
BASE COUNT      622 a    612 c    581 g    590 t
ORIGIN      
        1 atggcaacag ttaaccagct ggttcgaaag ccgcgagctc gtaaagtggc caaatctaac
       61 gttccggctc tcgaggcatg cccgtagaag cgtggcatat gcacacgcgt atacactact
      121 actccgaaga aaccgaattc agcgctgcgc aagctttgcc gcgtacgcct gaccaacggt
      181 ttcgaggtca cctcatatat aggtggtgaa ggacacaacc tgcaggaaca ctctgttatc
      241 ctgatcagag gcggccgcgt taaagatctg cccgggatcc ggtaccacac cgtccgcggc
      301 gctctagact gctccggagt aaaggaccgt cgacaggatc gatcgaaata cggtgtaaaa
      361 cgtccgaagg cctaatagaa gctagcttct tccgcttcct cgctcactga ctcgctgcgc
      421 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
      481 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
      541 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
      601 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
      661 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
      721 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcaatgctc acgctgtagg
      781 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
      841 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
      901 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
      961 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt
     1021 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     1081 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     1141 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     1201 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     1261 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     1321 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     1381 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     1441 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     1501 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     1561 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     1621 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     1681 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     1741 aaaaaagcgg ttagctcctt cggtcctccg attgttgtca gaagtaagtt ggccgcagtg
     1801 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     1861 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     1921 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     1981 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     2041 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     2101 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     2161 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     2221 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     2281 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgaattatt ctgaaatgag
     2341 ctgttgacaa ttaatcatcg aactagttaa ctagtacgca agttcacgta aaaagggtat
     2401 cgacc
//