From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: E. coli D-amino acid dehydrogenase and alanine racemase (dad
Date: 29 Sep 92 10:02:58 GMT
Distribution: bionet
Checksum: 14584    11

LOCUS       ECODADAX     4386 bp ds-DNA             BCT       29-SEP-1992
DEFINITION  E. coli D-amino acid dehydrogenase and alanine racemase (dad
            operon) genes, complete coding regions.
ACCESSION   L02948
KEYWORDS    D-amino acid dehydrogenase; Shine-Dalgarno sequence;
            alanine racemase; dad operon; dadA gene; dadX gene;
            protein binding site.
SOURCE      Escherichia coli (strain K-12) DNA.
  ORGANISM  Escherichia coli
            Prokaryotae; Gram-negative facultatively anaerobic rods;
            Enterobacteriaceae.
REFERENCE   1  (bases 1 to 4386)
  AUTHORS   Lobocka,M.B.
  TITLE     Organization and nucleotide structure of the dad operon of
            Escherichia coli K12, and its regulation
  JOURNAL   Thesis (1990)
  STANDARD  full staff_review
REFERENCE   2  (bases 1 to 4386)
  AUTHORS   Lobocka,M.B., Hennig,J., Wild,J. and Klopotowski,T.
  TITLE     Structure and expression of the dad operon of Escherichia coli K12,
            coding for the D-amino acid dehydrogenase and the catabolic alanine
            racemase
  JOURNAL   Unpublished (1992)
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     CDS             complement(1..1330)
                     /product="unidentified protein"
                     /note="putative"
                     /codon_start=1
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNYLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQ"
                     /citation=[1]
                     /citation=[2]
                     /partial
     RBS             complement(1336..1341)
                     /standard_name="Shine-Delgarno sequence"
                     /note="putative"
                     /citation=[1]
                     /citation=[2]
     protein_bind    1544..1564
                     /bound_moiety="CRP"
                     /note="putative"
                     /citation=[1]
                     /citation=[2]
     protein_bind    1607..1627
                     /bound_moiety="CRP"
                     /note="putative"
                     /citation=[1]
                     /citation=[2]
     RBS             1651..1654
                     /standard_name="Shine-Delgarno sequence"
                     /gene="dadA"
                     /note="putative"
                     /citation=[1]
                     /citation=[2]
     CDS             1660..2958
                     /function="oxidative deamination of D-amino acids"
                     /note="minor subunit"
                     /product="D-amino acid dehydrogenase"
                     /gene="dadA"
                     /evidence=EXPERIMENTAL
                     /codon_start=1
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKCGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
                     /citation=[1]
                     /citation=[2]
     RBS             2956..2961
                     /standard_name="Shine-Delgarno sequence"
                     /gene="dadX"
                     /note="putative"
                     /citation=[1]
                     /citation=[2]
     CDS             2968..4038
                     /EC_number="5.1.1.1."
                     /product="alanine racemase"
                     /gene="dadX"
                     /evidence=EXPERIMENTAL
                     /codon_start=1
                     /translation="MTRPIQASLDLQALKQNLSIVRQAATHARVWSVVKANAYGHGIE
                     RIWSAIGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISGRMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGRSPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHALTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALAVRVPVVTV"
                     /citation=[1]
                     /citation=[2]
BASE COUNT     1009 a   1084 c   1217 g   1076 t
ORIGIN
        1 tctgaatatt cggctccaga ttacttaagt tatattgcga cgataaccgg ttacggatct
       61 cccgataacc ttcttcatta tgaatagcgg aaatctccag ataattatgc cgatcgtcat
      121 ccagcacggt gaagaagcgg aaatcacgca tcactttcgg tgacaggaac tggctgataa
      181 aactctcatc tttgaaatca cgcatcgcga aatgcagcgt ttccagccag tcggaaccgg
      241 cgatatccgg gaaccagtat ttgtcttctt ccgttggcga ctgacaaatc cgtttaatat
      301 cctggaacat ggcgaacccg agggcatacg ggttgatgcc gctgtaccac gggctgttat
      361 aggggggctg gaagaccaca ttggtgtggc tgtgcaaaaa ctccagcata aaacgttccg
      421 ttactttccc ttcatcatac agatggttaa ggatggtgta gtgccagaag gtcgcccagc
      481 cttcgttcat cacctgagtc tgtttttgcg gataaaaata ctggctcacc ttacgcacaa
      541 tacgcaggat ttcacgctgc catgattcca gcagtggcgc atttttctcc ataaaataga
      601 gcaggttttc ttgtggttcg gacggatagc ggcgcgcttc agcaaccgtt ttctcttcct
      661 cgcgcttcgg cagggtacgc cagagcatat tgacctgact ttgcagatac tcttcgcgac
      721 ttttctgccg ggctttctct tcttgcagcg agattttttg cgggcgtttg taccggtcca
      781 cgccgtagtt catcagcgcg tggcacgagt ccagaagccg ttctacttca tcaacgccat
      841 aacgctcttc gcactcggta atatatttac gggcaaaaat cagataatcg acaatcgaac
      901 tggcgtcggt ccagctacgg aataagtaat tgtttttgaa gaaagagtta tgcccatagc
      961 aggcatgagc catcaccagc gcttgcatgg taatggtgtt ctcttccatc aggtaagcga
     1021 tacacgggtt agagttaatg acgatttcat aggccagtcc ttgctgaccg tgcttataca
     1081 gccgttcagt ctcgataaac tttttaccga atgaccagtg cggatagtta attggcatgc
     1141 cgacgctgga gtaggcatcc atcatctgtt ctgaggttat cacttcaatc tggtgcgggt
     1201 aggtatccag ccggtagagt ttcgccaccc ggtctatctc tgccagataa acatccagca
     1261 ggtcgaacgt ccagtcgggt ccatcgctca aacgtgtggt gtccttattc atagaatcga
     1321 tcgtcgccat acgcgcacct cattgttgtc ggcgctctct gtgtggagca cctcatttca
     1381 agcatagaac acctgttaaa aaccgcgtcg ccggagaatt tttttctttg cgatttctta
     1441 ttatcagagt gccactaatc cgcttctgaa cggaatttta tgctggataa aaagggcgtt
     1501 cagcaggaga tactaaagac gccatattgc cgcagagtca gggagatgtg accagcttca
     1561 ccataaaaaa gccgcatgtt gaataatatt ttcaactgag ttatcaagat gtgattagat
     1621 tattattctt ttactgtatc taccgttatc ggagtggcta tgcgagttgt catactggga
     1681 agtggtgtgg taggcgttgc cagcgcctgg tacttaaatc aggcaggaca tgaggtcacc
     1741 gtcattgatc gggagccggg ggccgccctg gaaaccagtg ccgcgaatgc cgggcaaatc
     1801 tcccccggat atgctgcacc gtgggcggca ccaggtgtgc ctttaaaagc gattaaatgg
     1861 atgttccagc gccatgcgcc gctggcggtt cgtctcgacg gtacgcagtt ccagttgaaa
     1921 tggatgtggc aaatgttacg taactgcgac accagccact acatggaaaa caaagggcgg
     1981 atggtgcgtc tggcggaata cagccgtgat tgcctgaaag cattgcgcgc cgaaaccaat
     2041 attcagtatg aagggcgtca gggtgggacg ctgcaactgt tccgtaccga acaacagtat
     2101 gaaaatgcga cccgcgatat cgccgtgctg gaagatgccg gcgtaccgta tcagctgctg
     2161 gaatccagcc gcctggcgga agtggagccc gcgctggcag aagtggcgca caaactgacg
     2221 ggcggcctgc agttacccaa tgatgaaacc ggagactgtc agctatttac ccagaatctg
     2281 gcgcggatgg cggagcaggc gggggttaaa ttccgcttta atacgcccgt tgaccaactg
     2341 ctttgcgacg gcgagcaaat ctacggcgtg aagtgtggcg atgaagtgat taaggccgat
     2401 gcgtatgtga tggcgtttgg ttcttactcg acggcgatgc tcaaaggcat tgttgatatt
     2461 ccggtgtatc cgctgaaagg ctactcgctg accattccaa ttgcgcagga agatggtgcg
     2521 ccggtatcca ccattcttga tgaaacctac aaaatcgcca ttacccgttt cgataaccgc
     2581 attcgtgttg gcggaatggc ggagattgtt ggttttaata ccgagctgtt gcaaccgcgt
     2641 cgtgaaacgc tggagatggt ggttcgcgat ctctatccac gcggcggtca tgtcgagcag
     2701 gcgactttct ggactggtct gcgcccgatg acgccagacg gcacgccggt tgtcgggcgt
     2761 acacgcttta aaaatctgtg gctgaatacc ggtcacggca cgctcggctg gacgatggct
     2821 tgcggttccg gtcagttgtt aagcgatctg ctctctggtc gcacgccagc gatcccatat
     2881 gaggatctaa gcgtagcgcg ctacagccgt ggatttacgc catcacgtcc gggccattta
     2941 catggcgcac acagctaagg aaacgagatg acccgtccga tacaggccag cctcgatctg
     3001 caggcattaa aacagaatct gtccattgtc cgccaggccg cgacgcacgc gcgcgtctgg
     3061 tcggtggtaa aagcgaacgc ttacgggcat ggtattgagc gtatctggag cgcgatcggg
     3121 gccaccgatg gctttgcatt gcttaacctg gaagaggcaa taacgttacg tgagcgcggc
     3181 tggaaaggac cgatcctgat gctggaagga tttttccatg ctcaggatct ggagatttat
     3241 gaccagcacc gcctgaccac ctgcgtacac agcaactggc agctcaaagc actgcaaaat
     3301 gcgcggctaa aagcaccgtt ggatatttat cttaaagtga acagtgggat gaatcggttg
     3361 ggcttccagc ccgatcgcgt gcttaccgtc tggcagcagt tgcgggcaat ggcgaatgtt
     3421 ggcgaaatga ccctgatgtc gcattttgcc gaagcggaac atcctgatgg aatttccggc
     3481 cggatggcgc gtattgagca ggcggcggag gggctggagt gtcggcgttc gttgtccaat
     3541 tcggcggcga ctctgtggca cccggaagcg cattttgact gggttcggcc tggcattatt
     3601 ttgtatggcc gttcgccgtc cggtcagtgg cgtgatatcg ccaataccgg attacgtccg
     3661 gtgatgacgc taagcagtga gattattggt gtccagacgc taaaagcggg cgagcgtgtg
     3721 ggctacggcg gtcgctatac tgcgcgcgat gaacagcgaa tcggcattgt cgccgcaggg
     3781 tacgccgacg gttatccgcg ccacgcctta accggtaccc ctgttttagt ggacggcgtg
     3841 cgcaccatga cggtggggac cgtctcgatg gatatgctag cggtcgattt aacgccttgc
     3901 ccgcaggcgg gtattggtac gccggttgag ctgtggggca aggagatcaa aattgatgat
     3961 gtcgccgccg ctgccggaac ggtgggctat gagttgatgt gcgcgctggc cgtacgcgtc
     4021 ccggttgtga cggtgtaact tgttgtaagc cggatcggag gcaacgtctt ctgggtgcaa
     4081 aaaaatcatc catccggctg gtcagcaact gtagttgtta atgtgacaga gccattgccc
     4141 atgatagtgt ccattaaaag gatggacact atttccccgg aacctgaact caccgcacag
     4201 gcgttctaca taaaacgctt acgcttcatt gttgactcga actcgacttc agataaatca
     4261 cgctttaccc ttgatggagc ctgtacatag atttgtgtaa ttgcctgatt ttgatatgtt
     4321 caattcaaca tcaaatgaag gttaaattat ggacgacaaa caattgcagg ctcaggctgc
     4381 gttcag
//