From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Brevibacterium sp. wide spectrum amidase (amiE) gene, complete cds.
Date: 12 Aug 92 10:00:29 GMT
Distribution: bionet
Checksum: 00375     6

LOCUS       BRLAMID      2641 bp ds-DNA             BCT       12-AUG-1992
DEFINITION  Brevibacterium sp. wide spectrum amidase (amiE) gene, complete cds.
ACCESSION   M76451
KEYWORDS    ATP-dependent protease; amidase.
SOURCE      Brevibacterium sp. (strain ) DNA.
  ORGANISM  Brevibacterium sp.
            Prokaryotae; Firmicutes; Irregular asporogenous rods.
REFERENCE   1  (bases 1 to 2641)
  AUTHORS   Soubrier,F., Levy-Schil,S., Mayaux,J.-F., Petre,D., Arnaud,A. and
            Crouzet,J.
  TITLE     Cloning and primary structure of the wide-spectrum amidase from
            Brevibacterium sp. R312: High homology to the amiE product from
            Pseudomaonas aeruginosa
  JOURNAL   Gene 116, 99-104 (1992)
  STANDARD  full staff_entry
COMMENT     sequence homology to Pseudomonas aeruginosa
FEATURES             Location/Qualifiers
     CDS             181..1218
                     /EC_number="3.5.1.4"
                     /product="amidase"
                     /gene="amiE"
                     /codon_start=1
                     /translation="MRHGDISSSNDTVGVAVVNYKMPRLHDRAGVLENARKIADMMIG
                     MKTGLPGMDLVVFPEYSTQGIMYNEEEMYATAATIPGDETAIFSAACREADTWGIFSI
                     TGEQHEDHPNKPPYNTLILIDNKGEIVQRYRKILPWCPIEGWYPGDTTYVTEGPKGLK
                     ISLIICDDGNYPEIWRDCAMKGAELIVRCQGYMYPAKDQQVMMSKAMAWANNCYVAVA
                     NAAGFDGVYSYFGHSAIIGFDGRTLGETGEEEYGIQYAQLSVSAIRDARENDQSQNHI
                     FKLLHRGYSGVHAAGDGDKGVADCPFEFYKLWVTDAQKAQERVEAITRDTVGVADCRV
                     GNLPVEKTVEA"
     CDS             1229..2284
                     /note="ORF 1;"
                     /product="ATP-dependent protease"
                     /codon_start=1
                     /translation="MPYITDMLRDRNSAATPPAEERSEPVPVGAFDARRLSKALSSKI
                     VGQQAAVDAVVRAISIAHVGATDPTRPLANILLVGPTGVGKTELVRRVAAELRSGPDD
                     LCRIDMNALAQEHYAASFSGAPPGYAGSKESFTLFDKNTVEGDPYTPGIVLFDEVEKA
                     DPTVLRALLQVLDNGELRLANGQQKISFRNSYVFLTSNLGSAAVAERRRSHLRQLADR
                     VRIDRPRHGHHLVQRALEKFFDPEFFNRIDETVILDEFDDQTAEQVTRLEIELITTRL
                     ARRGIDVEVDDSAVALLQRRGFDPVYGARGLRRTIRNVLADPVAGAVLDLRPVGTQPL
                     ALQARAVGDQIQVKKAP"
BASE COUNT      559 a    853 c    790 g    439 t
ORIGIN
        1 ggttcctgcc ttcctgatcc tgaccggcaa cttccacacc acgccagcgg tcgccgccgg
       61 ctgggccgga gcacttctgg ttctcctggg gcttgccaag atccttgccg cacccaaagc
      121 tgcagtaccc caaccccgtc cagtcttcaa ctgatccaca caaccaagga gtgaaagaca
      181 atgcgacacg gtgacatctc ctcgagcaac gacacagtcg gcgtagcggt cgtcaactac
      241 aagatgcccc gcctgcacga tcgcgccggc gtcctcgaga acgcccgcaa gatcgccgac
      301 atgatgatcg gcatgaagac cggcctcccc ggaatggacc tggtggtctt tcccgaatac
      361 tccacgcagg gaatcatgta caacgaggag gagatgtacg cgacagcggc caccatcccg
      421 ggcgacgaga ccgcgatctt ctccgccgcg tgccgcgaag ccgacacgtg ggggatcttc
      481 tccatcaccg gtgaacaaca cgaggaccat ccgaacaagc ccccgtacaa cacgttgatc
      541 ctcatcgaca acaagggcga aatcgttcag aggtaccgaa agatcctgcc ctggtgcccc
      601 atcgaagggt ggtacccggg agacacgacg tatgtcacgg aaggaccgaa gggtctgaag
      661 atctcgctca tcatctgcga cgacggcaac tacccggaga tctggcgcga ctgcgcaatg
      721 aagggcgccg agctgatcgt ccgctgccag ggatacatgt acccggccaa ggaccagcag
      781 gtgatgatgt ccaaggcaat ggcctgggcc aacaattgtt atgtggcagt tgccaatgcg
      841 gccggattcg acggcgtcta ctcctacttc gggcactccg cgatcatcgg attcgacggt
      901 cggacgctgg gtgagaccgg cgaggaggaa tacggaatcc agtacgcgca gctgtccgtc
      961 tcggccattc gtgatgcacg cgagaacgac caatcccaga accacatctt caagctcctc
     1021 caccggggct actccggagt ccatgcagcc ggtgacggag acaagggtgt tgcggactgc
     1081 ccgttcgagt tctacaagct gtgggtcacc gatgcgcaga aggctcagga acgcgtcgag
     1141 gccatcacac gagacaccgt gggcgtcgcg gattgccgcg tcggaaatct cccggtcgag
     1201 aagaccgtcg aagcgtaagg atcctgctgt gccgtatatc accgacatgc tccgcgaccg
     1261 gaacagtgca gcaacaccac cggccgagga acgatccgaa cccgtaccgg tcggggcctt
     1321 cgacgcgcgg aggctctcga aagccctgag ttccaagatc gtcggacaac aggccgccgt
     1381 cgacgccgtg gttcgtgcga tttcgatcgc gcatgtcggt gccacggatc cgacgcgtcc
     1441 actcgccaac atccttctgg tcggccccac cggcgtcggc aagacggagt tggtgcggcg
     1501 cgtggccgcg gaactgcggt cggggcccga cgatctgtgc cgcatcgaca tgaatgctct
     1561 ggcgcaagag cattatgcgg cgtcgttttc cggggctccg cctggatacg ccggcagcaa
     1621 ggagtccttc acactgttcg acaagaacac cgtcgagggc gatccgtaca cccccggaat
     1681 cgtcctgttc gacgaagtgg agaaggcgga ccccaccgtg ctgcgtgcgc tactgcaggt
     1741 cctcgacaac ggcgaactca ggttggcgaa cggtcagcag aagatctcgt tccgcaacag
     1801 ctacgtgttc ctgacctcga atctcggttc ggctgcggtg gcggaacgtc gacggtcaca
     1861 cctgcggcag ctcgccgatc gcgtccggat cgatcgacca cgccatggcc atcacctggt
     1921 ccaacgcgcg ttggagaagt tcttcgaccc cgagttcttc aaccgcatcg acgaaaccgt
     1981 gatcctcgac gagttcgacg accagaccgc ggaacaggtg actcggctcg agatcgaact
     2041 gatcacgact cggttggccc ggagaggcat cgacgtcgag gtggacgaca gcgccgtcgc
     2101 tctcctgcag cgacgcggat tcgatccggt gtacggcgca cgcggtctgc gtcggaccat
     2161 ccggaacgtc ctggccgacc cggtggccgg cgccgttctc gacctacgac cggtcgggac
     2221 acagccactc gccttgcagg cccgggcagt tggtgatcag atacaggtaa agaaagcccc
     2281 atgagtgctc gctcacgttg cggatcgaat ccgcaacgtg ggcgacacat ttcaaacatg
     2341 tccgcgtaac gccacctccc taggttgggt gcctgcacca caaagtgcac atgcacaggc
     2401 acccagggga agtgacgtca tgacagcagt gaacgagcag gtgtccgcag tcgcaccgga
     2461 ccaggcgaat cccggtcaga acaccgcact gaaggagacg ctggaggact acaccctccg
     2521 attcgcgcca cgcagctacc gcaaatgggg aaccggagtc gtcgcgacgt ccgcactcgg
     2581 tggaatcgcg tatctcgcgg acttctcgat cggcgccaac atcggcatct cgtacggtac
     2641 c
//