From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Bacillus subtilis lipase (lipA) gene, complete cds.
Date: 19 Aug 92 10:00:44 GMT
Distribution: bionet
Checksum: 50458     4

LOCUS       BACLIPASE    1495 bp ds-DNA             BCT       19-AUG-1992
DEFINITION  Bacillus subtilis lipase (lipA) gene, complete cds.
ACCESSION   M74010
KEYWORDS    lipA gene; lipase.
SOURCE      Bacillus subtilis (strain 168) DNA.
  ORGANISM  Bacillus subtilis
            Prokaryotae; Firmicutes; Endospore-forming rods and cocci;
            Bacillaceae.
REFERENCE   1  (bases 1 to 1495)
  AUTHORS   Dartois,V., Baulard,A., Schanck,K. and Colson,C.
  TITLE     Cloning, nucleotide sequence and expression in Escherichia coli of
            a lipase gene from Bacillus subtilis 168
  JOURNAL   Biochim. Biophys. Acta 1131, 253-260 (1992)
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     -35_signal      414..419
                     /gene="lipA"
     -10_signal      440..445
                     /gene="lipA"
     RBS             465..472
                     /gene="lipA"
     CDS             478..1116
                     /EC_number="3.1.1.3"
                     /product="lipase"
                     /gene="lipA"
                     /codon_start=1
                     /translation="MKFVKRRIIALVTILMLSVTSLFALQPSAKAAEHNPVVMVHGIG
                     GASFNFAGIKSYLVSQGWSRDKLYAVDFWDKTGTNYNNGPVLSRFVQKVLDETGAKKV
                     DIVAHSMGGANTLYYIKNLDGGNKVANVVTVGGANRLTTGKALPGTDPNQKILYTSIY
                     SSADMIVMNYLSRLDGARNVQIHGVGHIGLLYSSQVNSLIKEGLNGGGQNTN"
     terminator      1127..1155
                     /gene="lipA"
BASE COUNT      459 a    307 c    344 g    385 t
ORIGIN
        1 gtccaaatga cggaagctac ctgttgacgc tgctgtaaaa ccggagcaaa ggggattgta
       61 tttgccggtt ctgggaacgg gtctttatct gatgcagccg aaaaaggggc ggacagcgca
      121 gtcaaaaaag gcgttacagt ggtgcgctct acccgcacgg gaaatggtgt cgtcacacca
      181 aaccaagact atgcggaaaa ggacttgctg gcatcgaact ctttaaaccc ccaaaaagca
      241 cggatgttgc tgatcgttgc tcttaccaaa acaaatgatc ctcaaaaaat ccaagcttat
      301 ttcaatgagt attgaagaaa agaaggcgaa taagccttct tttttttggc tttttaggac
      361 caataatgac ctctgaatct taaaatttct ttaaaaataa gccaaaatta ccctttactt
      421 aattaatttg gtaacgtaat ataattggag aatttgttac aaaaaaagga ggatattatg
      481 aaatttgtaa aaagaaggat cattgcactt gtaacaattt tgatgctgtc tgttacatcg
      541 ctgtttgcgt tgcagccgtc agcaaaagcc gctgaacaca atccagtcgt tatggttcac
      601 ggtattggag gggcatcatt caattttgcg ggaattaaga gctatctcgt atctcagggc
      661 tggtcgcggg acaagctgta tgcagttgat ttttgggaca agacaggcac aaattataac
      721 aatggaccgg tattatcacg atttgtgcaa aaggttttag atgaaacggg tgcgaaaaaa
      781 gtggatattg tcgctcacag catggggggc gcgaacacac tttactacat aaaaaatctg
      841 gacggcggaa ataaagttgc aaacgtcgtg acggttggcg gcgcgaaccg tttgacgaca
      901 ggcaaggcgc ttccgggaac agatccaaat caaaagattt tatacacatc catttacagc
      961 agtgccgata tgattgtcat gaattactta tcaagattag atggtgctag aaacgttcaa
     1021 atccatggcg ttggacacat cggccttctg tacagcagcc aagtcaacag cctgattaaa
     1081 gaagggctga acggcggggg ccagaatacg aattaatgaa aaacaaaacc ttgaagaatg
     1141 ctattcttca aggttattct gctttcagca caatggtttt cgcagccata tcatgaacgg
     1201 tttgtttttt cttcgtaaat gcggcagtca aatagatcag gcgggagaac acatgcaccc
     1261 acgctatcag gtaacggaca atggcttgcg ggaaggatat ttttttatat gtttcgtccc
     1321 tcacgatttg cagcccgatg ctttttttgc ccagtgtgcc cttccaattt gtcagcggca
     1381 tcagcaaaaa atacacaatc agcatcaata tggcgacaat aatgacaccg gcggacccat
     1441 cgccaaacgt aaatccggct gccaaaatca ctgctgcgca atgattacat caagt
//