HDTenEightyP/NetNews huggingface dataset:
From: CZJ@CU.NIH.GOV
Newsgroups: bionet.xtallography
Subject: Deposition and Release of Coordinates
Date: 2 Oct 92 13:17:00 GMT
Distribution: bionet

I do not know how many of you read the weekly postings of the NIH
Guide to Grants and Contracts but in the Sep 11 issue there was a
bit of notable significance:

The entire Public Health Service (including the NIH) nas adopted
the IUCr resolution regarding the deposition and release of
coordinates.

Essentially the resolution states that

Provisions for delayed release.  It is the intention of this
policy that the deposition of data associated with a scientific
publication should occur concurrently with publication of the
article.  Nevertheless, provision is allowed for the authros to
request a delay in the release of the deposited data.  For
deposited coordinates this delay is not intended to exceed one
year from the date of publication.  for deposited structure
factors the requested delay can be up to four years from the date
of publication.

The entire text of the resolution can be found in Acta Cryst.
A45 658 (1989).

This follows the adoption of the same policy by the National
Institute of General Medical Sciences in 1990.  John Norvell at
NIGMS pioneered that effort.  This effort in turn followed the
pioneering efforts by GenBank/EMBL regarding the deposition of
sequence data.

The bottom line is that those of us at NIGMS who administer
grants in the area of structure determination are enforcing this
policy and have begun to withhold funding in cases of
noncompliance.

Jim
From: FGARBRECHT@mis.mcw.edu
Newsgroups: bionet.immunology
Subject: Calcium dependent immunoglobulin binding
Date: 27 Sep 92 18:44:17 GMT
Distribution: bionet
Organization: Medical College of Wisconsin (Milwaukee, WI)

I have been working with a murine monoclonal to human CD8 alpha which
is Ca++ dependent in its binding.  Has anyone else seen calcium
dependent monoclonals, let alone looked into the phenomenon?
Thanks
Fred Garbrecht
Medical College of Wisconsin
Organization: Queen's University at Kingston
Date: Sun, 27 Sep 1992 10:52:46 EDT
From: <FORSDYKE@QUCDN.QueensU.CA>
Newsgroups: bionet.immunology
Subject: Re: IgM aggreg(v)ation
Distribution: bionet

The people working with expression vectors in foreign cytosols find that
aggregation is a major problem. One solution seems to be to lower the
incubation temperature. Less aggregation occurs at lower temperatures, since
the aggregations are primarily entropy driven. Perhaps this might help your
problem of aggregation of IgM.                    Sincerely,
                                                            Don Forsdyke
From: cdds01@vaxa.strath.ac.uk
Newsgroups: bionet.immunology
Subject: Re: Activity of human IL 6 in other species.
Date: 2 Oct 92 12:37:35 GMT
Organization: Strathclyde University VAX Cluster


Dear ImmunoNetters,

Does anyone out there know if human IL 6 is active in ruminants,
in particular sheep?

If you don't know perhaps you may know who I could ask.

Best wishes
Pete Crilly
Newsgroups: bionet.immunology
From: annick@physiol.su.OZ.AU (Annick Ansselin)
Subject: source of RAN-1 ?
Organization: Department of Physiology, University of Sydney, NSW, Australia
Date: Thu, 1 Oct 1992 09:13:21 GMT

Does anyone know of a reliable source of RAN-1? I am unable to obtain it
commercially. There is a lab in La Jolla who make their own, but they do
not answer letters or faxes :-(
From: MPCHLAP@PLKRCY11.EARN (Darek Kedra)
Newsgroups: bionet.immunology
Subject: Subscribe
Date: 29 Sep 92 13:41:55 GMT
Distribution: bionet
Original-To: IMMUNOLOGY <immuno@UK.AC.DARESBURY>

subscribe Darek Kedra
Newsgroups: bionet.immunology
From: Edward.Visse@wblab.lu.se (Edward Visse)
Subject: LINSCOTT on internet????
Followup-To: bionet.immunology
Organization: Wallenberg Lab. - University of LUND
Date: Mon, 28 Sep 1992 09:58:09 GMT

Hi netters,

Well the header really said it. Is it available on internet?

Edward
From: drw@euclid.mit.edu (Dale R. Worley)
Subject: Re: Some questions on AIDS
Organization: MIT Dept. of Tetrapilotomy, Cambridge, MA, USA
Distribution: bionet
Date: Wed, 30 Sep 1992 16:18:59 GMT

In article <01GP4PS5ISPC0018CI@SASK.USask.CA> LEES@SASK.USASK.CA writes:
   1. Can AIDS be infected through used electrical shaver?
   2. How long can this virus survive in the air?
   3. Can this virus be killed by ethanol or detergent like soap?

Since noone has replied, I'll tell what I know:

The answers seem to be "No", "a very short while", and "Yes".  To
answer the questions you are probably interested in, it appears that
HIV is *hard* to transmit.  The only transmission routes that are
epidemiologically significant are blood transfustion, sexual
intercourse, and mother-fetus.  Even sexual intercourse is not a
highly effective transmission route, and seems to be strongly
suppressed if there are no other STDs present.

There have been various cases reported that have been infected by
other routes.  But these generally amount to less than 10 cases per
unusual route.  As a matter of personal safety, one should look twice
before crossing the street before worrying about other routes.

You can probably get more (and better) information from sci.med.aids.

Dale Worley		Dept. of Math., MIT		drw@math.mit.edu
--
From a signature in alt.stupid:
 Not long after Andy and I met, we were driving down Pennsylvania Avenue and
 encountered some or another noisy pinko demonstration.
 "How come," I asked Andy, "whenever something upsets the Left, you see
 immediate marches and parades and rallies with signs already printed and
 rhyming slogans already composed, whereas whenever something upsets
 the Right, you see two members of the Young Americans for Freedom waving
 a six-inch American flag?"
 "We have jobs," said Andy.
 -- From  "Parliament of Whores" by P.J. O'Rourke
Organization: Queen's University at Kingston
Date: Mon, 28 Sep 1992 09:14:21 EDT
From: <FORSDYKE@QUCDN.QueensU.CA>
Newsgroups: bionet.immunology
Subject: Journal Watch: Antigen dose-response reevaluation

Journal Watch (JW)

  Has anyone noticed the subtle reevaluation of quantitative aspects of
lymphocyte signalling over the last few months?

  (i) Williams and Beyers. Nature 356, 746-747 state: "It is generally
thought that TCRs must be crosslinked to activate a T cell, yet how is
this to occur through the TCR:MHCpep interaction? It seems most
improbable that adjacent MHC molecules will display the same peptides,
and to give a cross-linking signal two MHC molecules displaying the same
peptide would have to be tethered together."

 (ii)Tamura and Nariuchi. J. Immunology 148, 2370-2377 state: "These
results indicate that monovalent anti-CD3 is more efficient than divalent
anti-CD3 in induction of IL2 production and that cross-linkage of the
TCR/CD3 complex is not necessarily required for T cell clone activation."

(iii)Ucker, Meyers and Obermiller J. Immunology 149, 1583-1592 state:
"At low stimulatory dose [of antigen], cells do not respond but they
remain viable for extended periods. At higher dose (operationally in the
normal range), cells proliferate in a dose-dependent fashion. Finally,
at still higher dose, proliferation is inhibited and cells rapidly
become non-viable."  "In the simplest sense, it is possible to model the
cellular response as a function of the dose or strength of stimulation. One
high dose of stimulus gives proliferation, two give cell death."

  Any comments?
               Sincerely,
                         Don Forsdyke (Discussion leader)
Newsgroups: bionet.immunology
From: scostant@cc.curtin.edu.au
Subject: Mouse Immunoglobulin Allotypes?
Organization: Curtin University of Technology
Date: Sun, 27 Sep 1992 11:04:50 GMT



I am working with the following strains of mice; AKR, C57Bl/6J, CBA/H and BALB/c
I would like to know what the immunoglobulin gene allotypes are for these 
strains. ie. allotypes for gammaG1, gammaG2a, and gammaG2b?  The names of 
relavent journals would be appreciated. I have only been able to find the 
gamma2a allotype for the BALB/c mice but am looking for the others.

Thanks in advance,
Paul Costantino (Ph D student)
Curtin University of Technology
Perth, Western Austarlia

email (scostant@cc.curtin.edu.au)
From: karabinos@exmed1.dnet.gwdg.de ("EXMED1::KARABINOS")
Newsgroups: bionet.immunology
Subject: sub
Date: 26 Sep 92 07:45:24 GMT
Distribution: bionet
Original-To: immuno@uk.ac.daresbury

sub bionet.immunology
From: jasper@vax.path.ox.ac.uk
Newsgroups: bionet.immunology
Subject: MAb against Mouse Kk?
Date: 28 Sep 92 18:57:33 GMT
Organization: Oxford University Molecular Biology Data Centre
Nntp-Posting-User: jasper

Hi,

We are looking for a monoclonal antibody against the mouse Kk Class I antigen
which will react with Kk on Western blots (SDS denatured and reduced). We have
tried a variety of MAbs (I can let anyone have a list of those we have tried,
or that we know of that *don't* work for this) but cannot find one that works.
It would be useful, but not essential, if the same reagent worked on FACS and
immunoprecipitations. 

Many thanks for any help,

regards,    jasper

Jasper Rees
Sir William Dunn School of Pathology
Oxford University

jasper@vax.path.ox.ac.uk
tel  +44 865 275567
FAX  +44 865 275556
Xref: sparky bionet.immunology:130 bionet.general:1779
From: fike@rkna50.riken.go.jp (Fumio Ike)
Newsgroups: bionet.immunology,bionet.general
Subject: Re: Mouse Immunoglobulin Allotypes?
Keywords: mouse immunoglobulin allotype
Date: 6 Oct 92 07:48:14 GMT
Followup-To: bionet.immunology
Organization: Institute of Physical & Chemical Research (RIKEN) Saitama,Japan

In Article <1992Sep27.200450.1@cc.curtin.edu.au> Paul Costantino writes:

> I am working with the following strains of mice; AKR, C57Bl/6J,  CBA/H  and
> BALB/c  I would like to know what the immunoglobulin gene allotypes are for
> these strains. ie. allotypes for  gammaG1,  gammaG2a,  and  gammaG2b?   The
> names  of  relavent journals would be appreciated. I have only been able to
> find the gamma2a allotype for the  BALB/c  mice  but  am  looking  for  the
> others.
 
Maybe, you have received some informations from others.
What I know is as follows:

-----------------------------------------------------------------
		IgG2a	IgA	IgG2b	IgG1	IgD	IgM
strain name	(Igh1)	(Igh2)	(Igh3)	(Igh4)	(Igh5)	(Igh6)
-----------------------------------------------------------------
A		[e]	de	e	acdefgh
AKR		[d]	de	d	acdefgh
BALB/c		[a]	ah	ach	acdefgh
C3H/HeJ		a
C57BL/10	[b]
C57BL/6		b	b	b	b
C57BR/cd	a
C57L		a
C58		a
CBA/J		a
CE		[f]	f	f	acdefgh
DBA/1		c
DBA/2		[c]	cg	ach	acdefgh
NZB		e
RF		c
RIII		[g]	cg	g	acdefgh
SEA		[h]	ah	ach	acdefgh
SJL		b
SWR		c
-----------------------------------------------------------------
* [] means that the strain marked is type strain.

Reference: "Mouse immunoglobulin allotypes: description and special
           methodology", by L.A.Herzenberg and L.A.Herzenberg
           Handbookd of Experimental Immunology, Second Edition
           Edited by D.M.Weir, Blackwell Scientific Publications
           1973, Oxford, ISBN 0 632 09660 8

Hope this helps you.

ps. Pharmingen is selling several allotype-specific monoclonal antibodies.
    So, Pharmingen may have more detailed information.
    Pharmingen: 11555 Sorrento Valley Road, San Diego, CA 92121 USA
                Tel: (619)792-5730, FAX: (619)792-5238


Fumio Ike, PhD.                               E-mail:fike@rkna50.riken.go.jp
Animal and Cellular Systems Laboratory          TEL:+81-48-462-1111(ext5042)
The Institute of Physycal and Chemical Research (RIKEN)  FAX:+81-48-462-4681
From: MPCHLAP@PLKRCY11.EARN (Darek Kedra)
Newsgroups: bionet.immunology
Subject: subscribe
Date: 27 Sep 92 18:05:49 GMT
Distribution: bionet
Original-To: immunology <immuno@UK.AC.DARESBURY>

subscribe Darek Kedra
From: IAE@CU.NIH.GOV (Irene Anne Eckstrand)
Newsgroups: bionet.announce
Subject: Applications resulting from NIH-sponsored research
Date: 6 Oct 92 19:27:47 GMT

I would like the advice of scientists on the net regarding
some writing I am doing about the merits of basic research
support at NIH.  While most of us understand that products,
medical applications, etc. have their roots firmly embedded
in basic research, it is good to document this now and then.
Turns out now is a good time.

I am looking for good examples of biotechnologies and medical
applications (therapies, mapped genes,...) that are RECENT and
IMPORTANT and can be traced to basic research.  Your favorite
example would be gratefully appreciated!!

Irene Anne Eckstrand, Ph.D.
National Institute of General Medical Sciences
NIH
iae@cu.nih.gov
Tel: 301-496-7137
FAX: 301-402-3665
From: kristoff@net.bio.net (Dave Kristofferson)
Newsgroups: bionet.announce
Subject: net.bio.net offline this PM for about 4 hrs
Date: 3 Oct 92 00:36:09 GMT


We had a systems problem this Friday afternoon and mail to net.bio.net
was not being delivered for about 4 hours.  If you tried to respond to
the readership survey and couldn't get through, please resend your
response.  Thanks for your help and sorry for the inconvenience.

				Sincerely,

				Dave Kristofferson
				BIOSCI/bionet Manager

				kristoff@net.bio.net
From: kristoff@net.bio.net (David Kristofferson)
Newsgroups: bionet.announce
Subject: IMPORTANT!! - 1992 ANNUAL BIOSCI READERSHIP SURVEY - PLEASE RESPOND!!
Date: 2 Oct 92 01:30:38 GMT
Reply-To: biovote@net.bio.net

It's time to justify our existence once again by counting our
readership, so I'd ***GREATLY*** appreciate it if everyone who sees
this message respond to 2 or 3 simple questions (see SURVEY below).
Anyone who reads this message is requested to respond *regardless* of
their location or affiliation.  Only individual responses will be
counted, i.e. one e-mail/one person.  Duplicate votes will be
eliminated.  I will be reposting this message approximately weekly and
collecting responses up through 11/1/92.  Thanks in advance!!


------------------------------- SURVEY -------------------------------

(NOTE - You need not return this form.  Please simply reply with your
answers to the following questions.)

1) In what country are you located?

2) Did you see this message in your e-mail or by using some kind of
   newsreading software?

3) If you saw it using news software, what program do you use?

----------------------------------------------------------------------

Please use one of the addresses given below to send in your response
or respond to me directly at kristoff@net.bio.net.  This message is
also set with a Reply-To line to direct responses to the
biovote@net.bio.net address, but please use the Daresbury address
below (esp. in Europe) if you have any problems getting through to
biovote@net.bio.net.


Address to submit survey              Location        Network
------------------------              --------        -------
biovote@daresbury.ac.uk               U.K.            JANET
biovote@net.bio.net                   U.S.A.          Internet/BITNET


Thanks in advance for your time and help in sustaining BIOSCI!  The
results of this survey will be posted to BIONEWS/bionet.announce in
November.

				Sincerely,

				David Kristofferson, Ph.D.
				BIOSCI/bionet Manager

				kristoff@net.bio.net
From: kristoff@NET.BIO.NET (David Kristofferson)
Newsgroups: bionet.announce
Subject: Test, please ignore
Date: 2 Oct 92 00:24:37 GMT
Distribution: bionet

Test, please ignore.
From: GWU5042@gwuvm.gwu.edu (Dr. V. Hu)
Newsgroups: bionet.announce
Subject: CALL FOR ABSTRACTS
Date: 29 Sep 92 17:48:56 GMT


            XIIIth Washington International Spring Symposium
                          May 10-14, 1993
                  The George Washington University
                          Washington, D.C.

Topic:  THE CELL CYCLE:  REGULATORS, TARGETS, AND CLINICAL APPLICATIONS

The program will include talks by invited speakers as well as contributed oral
and poster presentations in the following major areas of research:

1.  G0/G1 Transition
2.  G1/S Transition (Control of DNA replication, etc.)
3.  Mitosis:  Induction and Mechanics  (Regulation of mitosis and changes in
    cell architecture)
4.  Cell Cycle-dependent Transcription/Post-transcriptional Regulation
5.  Cell Cycle Regulation in Development (embryogenesis, differentiation,
    senescence, apoptosis)
6.  Cell Cycle Regulation in Carcinogenesis and Other Diseases
7.  Clinical Applications of Cell Cycle Analysis and Intervention

For information and abstract materials, contact Dr. Valerie Hu, Dept. of
Biochemistry and Molecular Biology, George Washington Univ. Medical Ctr.
2300 Eye St., NW,  Washington, D.C.  USA  20037  Phone: (202) 994-3709
or internet: gwu5042@gwuvm.gwu.edu
From: kristoff@net.bio.net (Dave Kristofferson)
Newsgroups: bionet.announce
Subject: bionet.plants.maize does not pass - 28 YES, 15 NO
Date: 3 Oct 92 02:56:10 GMT


It appears that there was not much interest among the current
readership in a maize newsgroup.  Only 43 votes were received, 28 YES
and 15 NO.  Since the requirement is

The proposal must collect 80 YES votes in 30 days and the number of
YES votes must exceed the number of NO votes by at least 40 to pass.

this group does not pass.  If the discussion leader wants to contact
more colleagues and get them on the network, the idea can be raised
again for a vote in three months, but not sooner.

				Sincerely,

				Dave Kristofferson
				BIOSCI/bionet Manager

				kristoff@net.bio.net
From: coverton@sibelius.humgen.upenn.edu (Chris Overton)
Newsgroups: bionet.announce
Subject: Conference on Knowledge and Information Management
Date: 26 Sep 92 17:26:04 GMT



~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~

	       C A L L    F O R    P A R T I C I P A T I O N
				      
				  CIKM-92
		     First International Conference on
		    INFORMATION AND KNOWLEDGE MANAGEMENT
				      
			   November 8 - 11, 1992
		       Radisson Lord Baltimore Hotel
			  Baltimore, Maryland, USA
				      
	   ***** deadline for early registration is 9/25/92 *****
				   
CIKM-92 will provide an international forum for the presentation and 
discussion of research on the management of information and knowledge.
The scope of the conference will cover the integration database
technology, knowledge representation and reasoning, information retrieval,
and techniques for locating and accessing relevant data and knowledge in
very large, distributed information systems.

The conference will include 18 invited talks, 68 submitted papers, 24 poster
presentations, panel sessions and tutorials. The keynote address will be by
Gio Wiederhold (DARPA/Stanford) on "Intelligent Integration of Diverse
Information Sources".  Other invited speakers include Maria Zemenkova (NSF),
Amit Sheth (Bellcore), Judea Pearl (UCLA), Peter Buneman (Pennsylvania),
Doug Terry (Xerox Parc), Bob Robbins (Johns Hopkins), Joan Sullivan (NIST),
Bharat Bhargava (Purdue), David Waltz (Thinking Machines/Brandeis), Len
Gallagher (NIST), Ahmed Elmagarmid (Purdue), Richard Soley (Object
Management Group), Larry Reeker (IDA), Bruce Blum (Johns Hopkins), and
Il-Yeol Song (Drexel).

For additional electronic information, send email to CIKM-INFO@CS.UMBC.EDU
to receive an automatic reply containing registration forms and a draft of
the advance program.  To receive a copy of the advance program or for
detailed information on registration, contact: Maryann Sullivan, ISMM, Six
Forks Road, Raleigh NC 27615 USA, voice: 919-847-3747, fax: 919-676-0666.

CIKM-92 is sponsored by ISMM and the University of Maryland Baltimore County
and held in cooperation with AAAI, IEEE, and ACM (SIGART and SIGIR).

~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
From: POSTMASTER@gunbrf.nbrfnet.georgetown.edu
Newsgroups: bionet.announce
Subject: Restoration of Access to PIR Network Services
Date: 28 Sep 92 03:10:12 GMT


                 Announcement of Restoration of Access to
                   The Protein Identification Resource

After a system disk failure on 23 September 1992, the National Biomedical
Research Foundation has restored access to the Protein Identification Resource
Network Server and to the On-line System

Requests may again be sent to
  FILESERV@GUNBRF on BITNET
or to
  FILESERV@NBRF.Georgetown.Edu on Internet.
 
We regret any inconvenience this temporary loss of service may have caused.
------------------------------------------------------------------------
                                 Dr. John S. Garavelli
                                 Database Coordinator
                                 Protein Identification Resource
                                 National Biomedical Research Foundation
                                 Washington, DC  20007
                                 POSTMASTER@GUNBRF.BITNET
                                 POSTMASTER@NBRF.Georgetown.Edu
From: PARSONS_A@snd01.pcr.co.uk
Newsgroups: bionet.announce
Subject: BLAZE gone in a puff of smoke?
Date: 30 Sep 92 11:19:23 GMT
Distribution: bionet



Hi BioNetFolk,

I posted a BLAZE search to genbank.bio.net only to recieve a permission denied
message from the daemon?  Is this because of the termination of the contract 
with GenBank.  If so - does any one else know where I can find a BLAZE server?

Tony P.

Dr.Tony Parsons,	   VOX   : + 44 304 616871
Pfizer Central Research,   Internet   : parsons_a@snd01.pcr.co.uk
Ramsgate Road,             Compuserve : 100064,765
Sandwich,                "I love California, I practically grew up in Phoenix"
KENT CT13 9NJ UK          Vice President Dan Quayle


----------------------------------------------------------------------
NOTE added by moderator (D. Kristofferson) - Free BLAZE searches ended
when GenBank On-line Service shut down yesterday.  BLAZE is only
available on a commercial basis from IntelliGenetics and MASPAR now.
----------------------------------------------------------------------
From: kristoff@genbank.bio.net (David Kristofferson)
Newsgroups: bionet.announce
Subject: genbank.bio.net closing in 1.5 hrs.  BIOSCI -> net.bio.net
Date: 29 Sep 92 22:36:14 GMT
Distribution: bionet
Organization: GenBank Online Service

We are closing down GOS and the machine genbank.bio.net at
approximately 5 PM this evening.  The various addresses at
genbank.bio.net for the BIOSCI newsgroups and GenBank functions such
as Authorin will continue to function (they'll be handled by
forwarding through the new BIOSCI machine net.bio.net).

My hope is that the transition will go smoothly and transparently, but
these are the kind of events that occasionally give rise to problems,
e.g., mailing loops, bounced mail, etc., so your patience is
appreciated.  Please contact either Kenton Hoover or myself if you
notice a problem.  The best addresses to use during the transition if
you need to report a problem are the following:

Kristofferson:  kristoff@presto.ig.com
Hoover:         shibumi@presto.ig.com

				Sincerely,

				Dave Kristofferson
				GenBank Manager for 1 hr 25 min more

				kristoff@genbank.bio.net