From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: D.melanogaster gene for eIF-4A eukaryotic translation initiation
Date: 31 Dec 92 01:30:16 GMT
Distribution: bionet
Checksum: 10767 7

LOCUS       DROEIF4A     3774 bp ds-DNA             INV       16-DEC-1992
DEFINITION  D.melanogaster gene for eIF-4A eukaryotic translation initiation
            factor
ACCESSION   X69045
KEYWORDS    cytoplasmic; l(2L)162 gene; lethal; translation initiation factor.
SOURCE      Drosophila melanogaster DNA.
  ORGANISM  Drosophila melanogaster
            Eukaryota; Animalia; Metazoa; Arthropoda; Uniramia; Insecta;
            Pterygota; Neoptera; Holometabola; Diptera; Brachycera;
            Cyclorrhapha; Schizophora; Drosophiloidea; Drosophilidae.
REFERENCE   1  (bases 1 to 3774)
  AUTHORS   Dorn,R., Morawietz,H., Reuter,G. and Saumwesen,H.
  TITLE     Identification of an essential Drosophila gene which is homologous
            to the translation initiation factor eIF-4A of yeast and mouse
  JOURNAL   Mol. Gen. Genet. (1992) In press
  STANDARD  full automatic
COMMENT     *source: strain=oregon R; *source: stage=embyro & adult; *source:
            sub_strain=ssp. meigen; *source: chromosome=X 26A7-9;
               From EMBL    entry DMEIF4A;  dated 13-NOV-1992.
FEATURES             Location/Qualifiers
     exon            <1..389
                     /number=1
     intron          390..951
                     /number=1
     exon            952..1178
                     /number=2
     intron          1179..1950
                     /number=2
     exon            1951..2560
                     /number=3
     intron          2561..2653
                     /number=3
     exon            2654..>3774
                     /number=4
     CDS             join(387..389,952..1178,1951..2560,2654..2731)
                     /gene="l(2L)162"
                     /product="eukaryotic translationinitiation factor 4A
                     (eIF-4A)"
                     /codon_start=1
                     /translation="MDDRNEIPQDGPASMEPEGVIESTWHEVYDNFDDMNLREELLRG
                     IYGYGFEKPSAIQQRAIIPCVRGRDVIAQAQSGTGKTATFSIAILQQIDTSIRECQAL
                     ILAPTRELATQIQRVVMALGEYMKVHSHACIGGTNVREDARILESGCHVVVGTPGRVY
                     DMINRKLRTQYIKLFVLDEADEMLSRGFKDQIQDVFKMLPPDVQVILLSATMPPDVLE
                     VSRCFMRDPVSILVKKEELTLEGIKQFYVNVKQENWKLGTLCDLYDTLSITQSVIFCN
                     TRRKGGPTDPGDVYPQLHRLGHARRHGAA"
BASE COUNT     1049 a    950 c    788 g    986 t      1 others
ORIGIN      
        1 ttaaatattt atactggtgg aaaactttga tgggcgtaat atcgatagca aataaaatag
       61 cgaagtcaag cagccaagct ctgggtctgc aggcctgaac ttcaagtggc acctccaaaa
      121 taacgtaaaa atgtgcaaaa taatacaaat tgaattcaaa atatttgcat tattttatgc
      181 tttgtttttc tttaccatac acactgcgaa gcgcagcgaa attgccggat atcgctgaca
      241 tctggcgata gttggcgatc gatctatcga tagtcgctaa tgttttctta catcactaaa
      301 acttttgcaa gatggagcct cttttgcttg gttttaacaa atcagtagca gcacgtgtat
      361 aaaaagtgcc aaataatttt cagaaaatgt tagtatattc cctagattaa gctcggctgc
      421 tctcgcccgt tgacattgac acgtacgaca ctagggtcgt cccgctcgca cacgctcagg
      481 tgcggactct ctacgcttgt cgctcaaaaa ggctgcctag ctatcgcttt cggtctcata
      541 cgtaaagtca ccgcttcgca gagcgcatcg cagcacaaac gtaacggact aaaagcaaaa
      601 cgctccatag ctcgagaaaa caacacagtc ggcgttcggc cgaattctcc gttattcgtt
      661 ctagacaaat aggcaaacag ttgattgagt gaaccgggcc caagccgtcg aaattcagga
      721 tataagttta cctagtcaat tctttattgt tgataatgtg agtggttgcc aagcggcgct
      781 cgcggttttc gattctccct ccaccagcct tacttactta cttttcgctt ttctgccatt
      841 cgccataacc aacaattgtg gctggaaaat tttttacccc cgtgccgcta ctgcttttgt
      901 tgttgtttcc actgttccga ccccactaac gcattctcgc tttcttgcag ggatgaccga
      961 aatgagatac ctcaggatgg ccccgccagc atggaacccg agggcgtcat cgagtccacc
     1021 tggcacgagg tgtacgacaa cttcgatgac atgaacttgc gcgaggagtt gctgcgcggc
     1081 atctacggtt atggtttcga gaagccgtcg gccatccagc agcgcgccat cattccctgt
     1141 gtgaggggtc gcgatgtcat tgcccaggcc cagtcgggtg agtaaatggc tccgagtccc
     1201 ccatcatcat cttcaccatc atccccctcg atcagtcaca aagaaaaact ttcgagaatg
     1261 tgcaaaatcg gcgaaaaaac aacacgatga ctcagccgtg cgcctgtatt tctttgttga
     1321 aattccgctt tgcagcactc tctttcttct ccttatactc tctctcttcc gtccgctctc
     1381 ttcgattgtc ggcgtcgtct gttgcgcgcc acttttcgct gaccagctgc gtttcggttt
     1441 ctcggccgcc aggccagaag aaagttccca gaaagaaggt gctcttcgag cgctcttggc
     1501 gaattccccc tgctatgcgt ttttaaggtc atctgttcca aatgcgatct acctacattg
     1561 ctcattcggc actaatccat tttgcaggct ctgcaaaaga ggctctatat acatattaat
     1621 atagttcatt ctcaactcct gttttgtgtt ctggcacttg cacctgccta tcgatgtata
     1681 ggcagggatt agtggccagc tcgaccctga aatggcacat cagctacctg atgaaacgat
     1741 ggacaactgt ctttattcca ttgatctttc cgtcattcaa gtttacgcct aacagaattg
     1801 cataacaaat ggcgtactgg ttagtcgaaa attggaattt tgctgaattt ccataagaat
     1861 atcacatagt tagaaaagta attgtgtata gtccgtagca cacactattc tgcatactaa
     1921 ccatttttna cctttaatct cctccgtagg aactggcaag actgccacct tctcgattgc
     1981 tatccttcag caaatcgaca cgagcattcg cgagtgccag gcgctgatcc tggcccccac
     2041 tcgcgagttg gccacgcaga tccagcgcgt ggtgatggcg ctcggcgagt acatgaaggt
     2101 gcactcgcac gcctgcattg gcggcactaa cgtgcgcgaa gacgcccgca tcttggaatc
     2161 cggttgccat gtggtggtgg gcactcctgg ccgcgtctac gacatgatca accgcaagtt
     2221 gcgcacccag tacatcaagc tgttcgtgct ggatgaggcc gatgagatgt tgtcccgcgg
     2281 tttcaaggat cagatccagg atgtcttcaa gatgctgccc ccagatgtgc aggtcatcct
     2341 gctgtccgcc accatgccgc cggatgtgct cgaagtgagc cgttgcttca tgcgtgatcc
     2401 cgtcagcatc ctggttaaga aggaggaact gacccttgag ggtatcaagc agttttacgt
     2461 caacgtgaag caggagaact ggaaactggg caccctttgc gatctgtacg atacgctgtc
     2521 catcacccag tcggtaatct tctgcaacac ccgtcgcaag gtaaattcac tcattcttat
     2581 aattgtgtga agctggatta gtttcaatcg caaagccgat aagttgctaa caaaaatgtc
     2641 tacccattct tcaggtggac caactgaccc aggagatgtc tatccacaac ttcaccgtct
     2701 cggccatgca cggcgacatg gagcagcgtg atcgcgaggt catcatgaaa caattccgtt
     2761 cgggctcgtc tcgtgttctg attaccactg atttactggc gcgcggtatt gatgtgcagc
     2821 aggtgtcgtt ggtcatcaac tatgatctgc cctcgaaccg cgagaactac attcataggt
     2881 aagtgctcac cacctcgccc ctgaatctac tcagctatgt gctatactaa acatgatacc
     2941 atcactttca atctatttca gaattggtcg cggtggtcgt ttcggtcgca agggtgttgc
     3001 gatcaacttt attacagatg atgatcgacg aatccttaag gatattgaac agttctacca
     3061 cacaacaatt gaggaaatgc ctgctaatat tgccgatttg atttaaattt tttctgctga
     3121 gccgcaaaac aactaagaaa gaattctaaa caaaaagtga gtgagcaaaa cagcaaacaa
     3181 gaactgcgaa atcgataacc gagcgcaact ataaattatt caaagatcaa caagtcaagt
     3241 taaatattta ttattcaaat attaattcga aaaggcagcc gaccgaccac ccaaaaaaag
     3301 gactaaacca ggacagagca gcaacatctt cagcagcggc gcagagagta acatcagcag
     3361 cagcgaacgc agcaacacca ccaccaattg ccgattagcc ggaacccctc tgtatccatt
     3421 tagttctctg tgtatcatca attttaaaac tgcgtgtcaa ccagcaacaa caaatatata
     3481 caaatatata aaaaaaaaaa aacaaaactt aaaacaacat ttgcatctat ctaaaaaaag
     3541 taatatctga aaccaggtac aagataccac ttgctaagaa gagcgaggaa ctaaacgcaa
     3601 aagccacaat atcagaacac ctttttgccg cacctttgtt ttagcttttt ctagaactat
     3661 tgataaaaat atattaaaac aaaacaaaat acatatgtac acattatgtt taaatatacc
     3721 actcctaaaa aacagctgac aaccgaagca acattttacg ctaaacacga acac
//