From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Pseudomonas sp. plasmid DNA for 2-aminobenzoate-CoA ligase,
Date: 24 Jul 92 10:09:10 GMT
Distribution: bionet
Checksum: 55370    16

LOCUS       PSEPPLAS     8280 bp ds-DNA             BCT       24-JUL-1992
DEFINITION  Pseudomonas sp. plasmid DNA for 2-aminobenzoate-CoA ligase,
            2-aminobenzoyl-CoA monooxygenase reductase and 5 open reading
            frames
ACCESSION   X66604
KEYWORDS    2-aminobenzoate-CoA ligase;
            2-aminobenzoyl-CoA monooxygenase reductase.
SOURCE      Pseudomonas sp. DNA.
  ORGANISM  Pseudomonas sp.
            Prokaryotae; Gracilicutes; Scotobacteria; Pseudomonadaceae.
REFERENCE   1  (bases 1 to 8280)
  AUTHORS   Altenschmidt,U., Bokranz,M. and Fuchs,G.
  TITLE     Novel aerobic 2-aminobenzoate metabolism. Nucleotide sequence of
            the plasmid carrying the gene for the flavoprotein 2-aminobenzoate
            -CoA monooxigenase/reductase in a denitrifying pseudomonas sp.
  JOURNAL   Eur. J. Biochem. (1992) In press
  STANDARD  full automatic
COMMENT     *source: sub_strain=KB740;
            See also X63668
            From EMBL    entry PSPPLAS;  dated 16-JUN-1992.
FEATURES             Location/Qualifiers
     CDS             1..1812
                     /product="2-aminobenzoate-CoA ligase"
                     /codon_start=1
                     /translation="MCQVLCTSHVDTFARDRLPPSEQQQLSSLISISELGLVQTVRAV
                     FAARTVAAATAERQEPDYYPEHALHTKASASPQNEPDRKHSPHARCRLTIVPGSPGKS
                     LDPARYLKAANTALKKTKRDSKRHQQHGSKRKRPMHRTTKNEPNPPTLHKLRKINKTP
                     HRSQSSLHVLLTNTHKQELINVLMLSSLIKDVSAAQAPGYGYAAVASKPAATTGPTAV
                     GKAVDRLLSYVIAAVSRRTPLSTMPTQHSRRRHTPRGPATPMQAITHSYLMLCFLPQR
                     LRMCIAAVEDVLLSGLLPLQREREGRRRRRNGPPRPRPVRRVSSLPMREPTRVSRRWG
                     GRRSPGSRVDWSRRAPIRRATAPRAQTETPSSGPVDEPTAEEKEIEEKLCETDPSARG
                     QVHLGGKRHVRYGRRPMRKRCGRTARARQDRGDSRRSGGHVGRCLRGAGGRVPVLASF
                     SVAREANDVGAEARMDGLASHGGDAHGGDPRRVHLVQGLIWKRYRRCSTGSMRSMNGS
                     PMASTPSWSSFMRGSRSRRLTWWYEALVWKLTLAWDIGPGRSAVVSELSMASTIQTWL
                     SVLPSNARSLVNYARVPEAINLIATAGFSKIVLRFIA"
     misc_binding    19..36
                     /bound_moiety="FADH"
     misc_binding    469..486
                     /bound_moiety="NAD(P)H"
     CDS             1874..2692
                     /note="ORF3"
                     /codon_start=1
                     /translation="MIPAWKSGRVIVTNVRGVSAQRFAHVLNVDESEGGDVIHVDTSN
                     QRGRLRMATWFHWAPPGALVFVDEAQAIWPKAWTQKDLQVLDYPGGSAQAEADDRPHC
                     YSIAWEMHRHWNWDFILTTPNINKLRDEYRGCADGAYKHKNLALIGLSGPLRRRFPPP
                     PDDTGKAQSDFISITRKRCRHMSSSATTLQPPAKSPTPSAARLFGRFRAWYCWSLSFY
                     VSAFLLLLVRCLRSSAGKGLHLLRLSGRGLLGLLLWVSRKVLLRLTVFLLVAGC"
     CDS             2828..5020
                     /product="2-aminobenzoyl-CoA monooxygenase"
                     /codon_start=1
                     /translation="MRIVCIGGGPAGLYFAILMKLNPAASSSGSLRVRGLLRRLQRET
                     ASGRAGRTLDERPPRPAARSAGVSLLPIAGTNQGIPPVGRKRSPEVEDCDAGADRERT
                     RRYQCVRAHGDRRVRGILPVVAKAVSIVLLRVADVLPRSGRLFLDGFQRNAQLVGAGV
                     RGPSRRGRARPCRGRPRCIRACVDRKSRSDGLGTGIYRSASARWRGWFPVRPAWLGRV
                     RIVRGGRSRARSSAADLFAGAREVEGGAHLAASANMYSAVCIASSSSSVKYSAAARCS
                     PLIQSSTRNSPQWASSITLTRMSPSFRVSMIFSSLMALSSWVIGDRGFAPGAPRRHQT
                     AAESNSIAWRGRCGRASPMRGGLCATGRCSAAPRARRAQRSPGTSDVPRTLRLADALS
                     SSPVPATGVPEPEPLRVFFLPAFKLFRRFPPFAPSPSPLASGNPTTKGMQGREAGEAF
                     TLAFPTCRVTRRRFRAKATGRAGSRTVEERKATEDARVFSGSSDRRARGKRSAQGRQA
                     RRGKGCEAGPRASPKGMSRQANPYQPGARVRAAPLNPPGNPRETPVKTPENPVQPPEK
                     FRENPGQTPENAFPMLPDWSGPLRFLYWRLRMARGVSRPSPRTWHRRIAAEKKRLLDA
                     GVDFFELHAVCVILRRSEYSVAAKQARDFLAKRADCVAVSPADLASLLDSVLVMVWAA
                     VLGAHVLGFLVTELLAFVADRAPSSAQLPRKPEPPSLVCSGIKAAVSR"
     CDS             5800..6606
                     /note="ORF4"
                     /codon_start=1
                     /translation="MRAEVFVSLVRVRGVAEECANFFATPRRDRAFYCFDELPPAVIL
                     SSERLRLAVYQDALEFGAGQRARWVLERLQVVLMSARVVLHPFLASAELPFMRLRRAC
                     FWQLADTDFRHAAYWRVQVEECVLQLVAWLMDGNGSPEAAQRCREAVTRAMVSGWSDA
                     AYRRVQVEVPAGGVCPSQHVQVKSAVESHFETVGSGGHPVSGVSLFDVSGSAGASSEC
                     HRARATMSTPEGAGETPQGQGCAARSAERPQSSHRAPVGKPGVFDLGASL"
     CDS             6630..7748
                     /note="ORF5"
                     /codon_start=1
                     /translation="MDIAQRHDGVDLPIVSDGVRVVGESFEEMTDATGRKWCVLTPHA
                     DGTRYTVRRKQSRGSFDTSLVCRCDGQKVELSGNVGRYNRADNVWNYDLAETLQKASD
                     YVAQEYGLPGFTAGECRVKPSLSKHDYDLGLWSEWTGAVFRELHVTRNYYVGSEALAC
                     EAMRYMGEQRASRVAKGKYGDETIVFGKQHGKLHKRLVVYRKAQEMLAHAKGDEAKKR
                     VKASEEYQFARDMGLIRIECKWGRDFLRDNGLRFVGDANMAKVISIFERESGFLLNVS
                     ADRAARVVSEMPSKIRSSALHWIRGDDLRVLLPRATYFRHVKALREFGIDATEPRSKQ
                     EGAAETLQRLLDDLPQFELRELPAPDWYGLPDLSRRAA"
     misc_feature    7748..8176
                     /note="ORF6"
     CDS             7999..8238
                     /note="ORF7"
                     /codon_start=1
                     /translation="MKKQLQLRLAQLSVGVSGVMAAGLASAAVDTTAVGTAITAAQGS
                     AEYGRAAWLMRLGRLARASGRLWSCVLSIYYRATR"
BASE COUNT     1469 a   2437 c   2706 g   1668 t
ORIGIN
        1 atgtgccagg tcttgtgcac tagccacgtc gacaccttcg cgagggatcg actgccacca
       61 agcgaacagc agcagctatc gagtctgata tctatctcgg agctcgggct agtgcagacg
      121 gttcgagccg tctttgcagc gcgtacagta gcggcggcaa cggccgagcg gcaggagcca
      181 gactactacc cggagcacgc gttgcacact aaagcgtccg cgagcccgca aaacgagccc
      241 gaccgcaagc acagcccgca cgcccgctgt cgcttgacta tcgtgccggg aagccccggc
      301 aaaagtttag accccgcgcg ctacctgaaa gccgcaaaca ccgcattaaa gaagaccaag
      361 cgcgacagca agcgccacca gcagcacggc agcaaacgca aacggcctat gcatagaacg
      421 acaaaaaatg aaccgaaccc ccccacgctt cataagctca ggaagatcaa taaaaccccg
      481 caccgttcgc agagttcgct gcacgtgctg cttaccaaca cgcacaagca agagctcatc
      541 aatgtgctca tgcttagttc tttaataaag gatgttagcg cggcacaggc cccaggttat
      601 ggttatgctg ctgtagcgtc caaaccagcc gcaaccacag gtccaacggc cgtcggaaag
      661 gccgtcgata ggttgctgag ttacgtaatt gcggcggtca gcaggaggac ccctttgagc
      721 accatgccta cccaacacag taggaggcgg cacactcccc gcggtccagc cactcccatg
      781 caagctatta cgcatagcta ccttatgtta tgttttctac ctcagagact acgtatgtgc
      841 atagctgctg tggaggatgt attactgtcg ggcctcctgc cgcttcagcg cgagcgggaa
      901 gggcggagac gtagacgaaa cggcccgccc cgtccgcgcc cggtccgcag ggtgtcgtca
      961 ttgccgatgc gggaaccaac cagggtatcc cgccggtggg gcggtaggcg ttcaccagga
     1021 agtcgcgttg attggtcacg ccgggcgccg atcaggcggg caactgcacc gagagcacag
     1081 accgaaacgc cttcgtcggg tccggtcgat gaacccaccg cagaagaaaa ggaaatcgag
     1141 gaaaagctct gcgaaaccga tccgagtgcc cgcgggcaag ttcacttagg cggaaaaagg
     1201 cacgttcgat atgggcgccg gccaatgcgg aaaaggtgcg gccgaacagc gcgtgcgcga
     1261 caagatcgcg gagattcgcg ccgaagcggc gggcatgtgg ggcggtgtct ccggggcgcg
     1321 ggcgggcgcg ttcctgtact cgcgtccttc tcggtcgctc gggaagcaaa cgacgtggga
     1381 gctgaagccc gaatggatgg actggcttcg cacggtggtg atgctcatgg cggcgatcct
     1441 cgccgcgttc atcttgttca gggactgata tggaagcggt acaggcgttg ctcgacgggc
     1501 tcaatgcgat ctatgaatgg atcgccaatg gcatctacac cttcttggtc gagttttatg
     1561 cgtgggtcgc ggtcaaggcg cctgacgtgg tggtatgagg cgctggtctg gaaactgacc
     1621 ttggcgtggg atatcggccc cggccggtcg gcggtcgtct ctgagctgtc gatggcttct
     1681 acgattcaaa cctggttgtc ggtgctgccg tccaatgcgc gttctctggt caattacgct
     1741 cgggttccgg aagcaatcaa cctgattgcg acggcgggtt tctcgaaaat cgtgttgagg
     1801 ttcatcgcat gagcatcaag attcactacg ggccgaacgc tcgtataaga ccgcgggcgc
     1861 gttggccgat gacatgatcc cggcttggaa gtcggggcgg gtcatcgtca cgaatgtccg
     1921 cggcgtctcg gctcagcgct tcgcccatgt gctcaacgtc gatgagtcgg aagggggtga
     1981 cgtgattcac gtcgacacca gcaaccagcg aggtcgtctg cgaatggcta cgtggttcca
     2041 ttgggctcca ccgggggcgc ttgtctttgt cgatgaggcg caagcgatct ggcccaaggc
     2101 gtggacgcaa aaggatttgc aggtgctcga ttatccgggc ggttctgcgc aggctgaagc
     2161 ggacgatcgc ccgcattgct attcgatcgc gtgggagatg caccgtcact ggaactggga
     2221 tttcatcctc accacgccca acatcaacaa gttacgcgat gaataccgcg gctgcgccga
     2281 tggggcctac aagcacaaga acttggcgct gatcggcctg tccgggccgc tacgtcgaag
     2341 gtttccaccg ccgcctgacg ataccgggaa ggcgcaatcg gatttcatct ccattacccg
     2401 caaaaggtgc cgtcatatgt cttcaagtgc tacgactcta cagccaccgg caaagtctcc
     2461 gacaccatca gcggcacgcc tctttggaag attccgcgcg tggtactgct ggtcactgtc
     2521 cttctatgta tcggcctttt tgttgcttct cgtccggtgc ctaaggtcct cggcggggaa
     2581 ggggttgcac ctgctgcgcc tgagcggtcg cggcctgctc ggtctgctcc tgtgggtgtc
     2641 gcgcaaagtg ctcctgcggc tgacggtgtt cctgctcgtc gctggctgtt gaaagcggcg
     2701 agctcttctg cactagcgcc cgatccgtgg agggtgcgtg ctcgctacga gcagcttgtc
     2761 atggggcgcg tcagcgtatt gtggtgacgt tctcccacct gaccgggtaa ggtcgttatt
     2821 accggcgatg agaatcgttt gcatcggtgg tggtcccgct gggttgtact tcgccatact
     2881 catgaagctt aatccagcgg caagcagcag cgggtcatta cgtgtgcggg gcctgctgcg
     2941 ccgccttcag cgcgagaccg cgagcggaag ggccgggagg acgttagacg aacgcccgcc
     3001 ccgtcccgcc gcccggtccg caggtgtgtc gttattgccg atcgcgggaa ccaaccaggg
     3061 tatcccgccg gtggggcgta agcgttcacc agaagtcgaa gattgtgacg ccggcgccga
     3121 tcgagagcgc accaggcgtt accaatgcgt tcgggcgcat ggtgacaggc gcgtgcgtgg
     3181 tatactcccg gttgtcgcca aagcggtgtc aatcgtgctt cttcgcgtag ccgatgtatt
     3241 gccacgcagc ggccgtcttt tcttggatgg ttttcagcgt aacgcgcagc tcgtcggcgc
     3301 cggcgttcgc gggccatcgc ggcgcgggcg tgctcgacct tgtcggggtc ggccccgatg
     3361 tattcgcgct tgcgtcgacc gcaagagtcg gtcggatgga ttaggtacag gtatttaccg
     3421 atccgccagt gctcgctggc gcggatggtt cccagttcgg ccagcttggt taggtcgcgt
     3481 ccgcattgtt cgcggtggtc ggtccagggc gcgcagcagc gcggcggatc tcttcgcagg
     3541 cgcgcgcgag gtcgaagggg gtgctcattt ggcggcctcg gcgaacatgt attctgccgt
     3601 ctgcatcgct tcgagctcgt cgtcggtgaa gtactcggcg gcggcgcggt gctcgccgtt
     3661 gatccagagc tccacgcgga attcgcccca gtgggcatcg tcgatcacat tgacgcggat
     3721 gtctccgtct ttcagggtct cgatgatttt ctcgtctctc atggcgttgt cctcgtgggt
     3781 tattggtgac agggggtttg caccaggagc gccgcggcgt caccaaacag cggcagaatc
     3841 caactcgata gcttggcgcg gacgatgcgg gcgggcttct cccatgcgcg gaggtctttg
     3901 cgcaactgga cgatgttcgg cagcaccacg cgcgcggcgt gcccaaagaa gtccaggcac
     3961 atctgacgtg cctcgaactc ttcgccttgc ggatgcttta tcttcatcgc ctgtccctgc
     4021 taccggggtc cctgagcctg agcccctccg ggttttcttc cttccggcgt tcaaactgtt
     4081 tcgccggttc ccaccgttcg ccccatcgcc ttcgcccttg gcgtcgggga acccgacaac
     4141 caagggaatg caagggcgcg aagcgggcga agcctttacc cttgcattcc cgacttgtcg
     4201 ggttacacgt cgccggttta gggcgaaggc gacggggcgg gcggggtcgc ggacagtgga
     4261 agaacggaag gccacagaag acgctcgcgt cttctctgga tcaagcgacc gcagggcgcg
     4321 cggcaagcgg agcgcgcagg gccgacaggc ccggaggggg aagggatgcg aagcggggcc
     4381 gcgggcctcc ccgaagggga tgagccgaca ggcgaacccg taccagcccg gtgcgcgcgt
     4441 gcgcgcagcc cccctgaacc ccccgggaaa cccccgggaa accccggtca aaaccccgga
     4501 aaatcccgtc caacccccgg aaaagttccg ggaaaacccc ggacaaaccc cggaaaatgc
     4561 ctttccgatg ttgcccgact ggtccggccc gctgcgcttc ctgtactggc gtctgcgcat
     4621 ggcgcggggc gtgtcgcgcc ccagcccgcg cacctggcat cgtcggatcg cggccgaaaa
     4681 aaaacgcctg ctggatgcag gcgtcgattt tttcgagctt cacgcggtgt gcgtgatcct
     4741 acgacgttcg gagtattccg ttgctgccaa gcaggcgcgt gattttctcg ccaagcgtgc
     4801 ggattgcgta gcggtgtccc cggccgatct cgcgtccctg cttgattcgg tgctggtgat
     4861 ggtttgggcg gctgtcctcg gcgctcatgt gctcggcttc ttggtaacgg agctgcttgc
     4921 gtttgtcgcg gatcgtgcgc cgtcgtcagc tcagctgccg cgcaagccag aaccgccatc
     4981 attggtatgt agtggaatca aggccgctgt aagccgttga tgcgtaagca ttcttcgggg
     5041 cgactgtcga aaagaatccg acaaccatcg cgagactcgc agcggccgca aactttgacc
     5101 agaaggcgcg tttcttctcg tctttttcgc gctcggcgtt cgcctgggct atgacgatca
     5161 tcggatctag gtcgagcagt tccgcgacct tggcggcggt gtagtcgtcc atcacgcgtt
     5221 gccctgtcat gtagcggctt atgacgggcg tcgagctctc tagaagtctg gctagttcag
     5281 cgtcgcattt gagctcgtgc ttggctttta cggcctgcag aaattctttt gtcatcatcc
     5341 ctagtccctc cgtcgtagct gctgcgcacg ttacgcggct tgctaccgct tgacaactac
     5401 caggtgggag tgatagcgtc cgttccgcta tcacgtggta gcacttcctt acattcgagg
     5461 tatagaccat gagcgccgtg cagccgatcc cgagtcagtc cccccaaacc acgatcaagc
     5521 cggggcaagt cctcgtcgtt ggccgctgtg tcagcgccaa gcgccagggg cagctgtacg
     5581 ttcacctcat cgtcatgccg gcgcccgatc cgtattcgag ccccgcgacg gtcgaagtgc
     5641 tggccaagac gcgacaggcc gattcagaac aggacgtgcg cctgctctgc cgcctcggtg
     5701 gctaccgtcg cagctacaag agcgtcgatc gcgagacggg cgaagtgcgc aacgtccaga
     5761 ccgccgacaa caagctgttc cttctcgaag actgaagcca tgcgggccga ggtttttgtg
     5821 tcgctcgttc gcgttcgggg cgttgctgag gaatgcgcga acttcttcgc cacgccccgg
     5881 cgcgatcggg cgttttactg cttcgatgag ttgccgccgg ccgtgatcct ctcgtcggag
     5941 cggttgcggc tcgccgtcta tcaggatgcg ctcgagtttg gcgccggcca gcgtgcccgg
     6001 tgggtgctgg aacggttgca ggttgtactg atgagcgcgc gcgtcgtgct ccatcccttt
     6061 ctcgcctcgg ccgagctgcc gttcatgcgg ctccgtcgtg cctgcttctg gcagctcgcg
     6121 gacaccgact ttcggcatgc ggcctactgg cgtgttcagg tggaggaatg cgttctccag
     6181 ctggtcgcgt ggctgatgga tggaaacggg tcgccggaag cggcgcaacg ctgccgtgag
     6241 gccgtgacgc gggcgatggt ttccggctgg tcggacgccg cttatcggcg cgtccaggtg
     6301 gaggttcccg cgggcggcgt ctgcccttca cagcacgtac aagtaaagag tgctgtggag
     6361 tctcattttg agactgtcgg ttctggtggg catcccgttt ccggggtttc gcttttcgac
     6421 gtttccgggt cggctggcgc gtcttcggag tgccatcggg cgcgcgcgac gatgagcacg
     6481 cccgaagggg cgggcgaaac cccgcagggg caaggatgtg cagcgcgtag cgcggaacgt
     6541 ccgcagtcga gccaccgcgc gcccgtggga aagccaggag tttttgacct tggggcttct
     6601 ctgtgacgtc ctcgaccttc atcgactgga tggacattgc gcaacggcat gacggcgtgg
     6661 atttgccgat tgtgtcggac ggggtccggg tcgtcggtga gtcgttcgag gaaatgacgg
     6721 acgcgacggg gcgcaagtgg tgcgtgctca ctccgcatgc ggacgggacg cgctacacgg
     6781 tgcgccgcaa gcaaagccgg gggtcgttcg atacgtcgtt ggtgtgccgc tgcgacgggc
     6841 agaaggtcga gctctccggc aacgtcggcc gttacaaccg cgcggacaac gtgtggaact
     6901 acgacctcgc cgagaccctg cagaaggcca gcgactacgt cgcgcaggaa tacgggctac
     6961 cgggcttcac ggcgggcgaa tgtcgcgtca agccgtcgct ctccaagcat gactacgacc
     7021 tcgggttgtg gtccgaatgg accggggcgg tgttccgcga gctgcacgtc acgcggaact
     7081 actacgtcgg gtcggaagcg ctcgcgtgtg aggcgatgcg ctacatgggc gagcagcgcg
     7141 cgtcgcgcgt cgccaagggc aaatacgggg acgagaccat cgtcttcggc aagcaacacg
     7201 gcaagttgca caagcggctt gtggtgtatc ggaaggctca ggaaatgctg gcacacgcca
     7261 aaggcgatga agccaagaag cgcgtaaagg cgagcgagga ataccagttc gcgcgggata
     7321 tggggctgat ccgaattgag tgcaagtggg ggcgggactt cctacgggat aacggattgc
     7381 ggtttgtggg ggatgccaac atggcaaagg tcatttcgat tttcgagcgc gaaagcgggt
     7441 ttctgttgaa cgtgtcggcc gatcgcgcgg cgcgcgtggt gtcggagatg ccgagcaaga
     7501 tcaggtcgtc ggcgctgcac tggattcgcg gggatgacct gcgggtgctg ctgccgcgcg
     7561 ccacgtattt ccgccacgtc aaggcgctgc gtgagttcgg cattgacgcg acggaaccgc
     7621 gcagcaaaca ggaaggggca gcggaaacgc ttcagcgctt gttggatgac ctcccgcagt
     7681 tcgagctgcg cgagctgccg gcgccggatt ggtacggcct ccccgatctg tccaggcggg
     7741 cggcgtgatg gaacgggagt cgttttgatg gcaacgctgt gccttgaagc gcatccagat
     7801 ggttcggtgc atgtcccggc gggcggcacc tgtgaattta tggcggttac accctcggcg
     7861 ctgtacagca tcgaggaatt cgattcagag gctttctcat acggcgtgat gggaacgctt
     7921 ttgatgttcg cggtgggcgc gggtatcgtc ctgcttatca atgtgcttca gaagggcacg
     7981 tgtttaggag tgtgtgaaat gaaaaagcaa ctgcaacttc gtctggctca actcagcgtg
     8041 ggtgtctcgg gtgtcatggc tgccggtctg gcctccgctg ccgtggatac gaccgcggtc
     8101 ggcaccgcga tcactgctgc gcaaggctcg gccgaatacg gtcgggcggc atggctcatg
     8161 cgactcggcc gtctagcacg cgcgtctggc cggttgtggt cctgtgttct ctcgatctac
     8221 taccgagcca ctcgctgaca gacaagctgc tcgcatttct cggaggtcga gcacgttcgt
//