From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human nitric oxide synthase mRNA, complete cds.
Date: 12 Sep 92 10:02:42 GMT
Distribution: bionet
Checksum: 52699     8

LOCUS       HUMNIOXSYN   4077 bp ss-mRNA            PRI       12-SEP-1992
DEFINITION  Human nitric oxide synthase mRNA, complete cds.
ACCESSION   M93718
KEYWORDS    nitric oxide synthase; vasodilator.
SOURCE      Homo sapiens umbilical vein cDNA to mRNA.
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (sites)
  AUTHORS   Janssens,S.P., Shimoushi,A., Quertermous,T., Bloch,D.B. and
            Blach,K.D.
  TITLE     Cloning and expression of a cDNA encoding human endothelium-derived
            relaxing factor/nitric oxide synthase
  JOURNAL   J. Biol. Chem. 267, 14519-14522 (1992)
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     CDS             47..3658
                     /product="nitric oxide synthase"
                     /codon_start=1
                     /translation="MGNLKSVAQEPGPPCGLGLGLGLGLCGKQGPATPAPEPSRAPAS
                     LLPPAPEHSPPSSPLTQPPEGPKFPRVKNWEVGSITYDTLSAQAQQDGPCTPRRCLGS
                     LVFPRKLQGRPSPGPPAPEQLLSQARDFINQYYSSIKRSGSQAHEQRLQEVEAEVAAT
                     GTYQLRESELVFGAKQAWRNAPRCVGRIQWGKLQVFDARDCRSAQEMFTYICNHIKYA
                     TNRGNLRSAITVFPQRCPGRGDFRIWNSQLVRYAGYRQQDGSVRGDPANVEITELCIQ
                     HGWTPGNGRFDVLPLLLQAPDEPPELFLLPPELVLEVPLEHPTLEWFAALGLRWYALP
                     AVSNMLLEIGGLEFPAAPFSGWYMSTEIGTRNLCDPHRYNILEDVAVCMDLDTRTTSS
                     LWKDKAAVEINVAVLHSYQLAKVTIVDHHAATASFMKHLENEQKARGGCPADWAWIVP
                     PISGSLTPVFHQEMVNYFLSPAFRYQPDPWKGSAAKGTGITRKKTFKEVANAVKISAS
                     LMGTVMAKRVKATILYGSETGRAQSYAQQLGRLFRKAFDPRVLCMDEYDVVSLEHETL
                     VLVVTSTFGNGDPPENGESFAAALMEMSGPYNSSPRPEQHKSYKIRFNSISCSDPLVS
                     SWRRKRKESSNTDSAGALGTLRFCVFGLGSRAYPHFCAFARAVDTRLEELGGERLLQL
                     GQGDELCGQEEAFRGWAQAAFQAACETFCVGEDAKAAARDIFSPKRSWKRQRYRLSAQ
                     AEGLQLLPGLIHVHRRKMFQATIRSVENLQSSKSTRATILVRLDTGGQEGLQYQPGDH
                     IGVCPPNRPGLVEALLSRVEDPPAPTEPVAVEQLEKGSPGGPPPGWVRDPRLPPCTLR
                     QALTFFLDITSPPSPQLLRLLSTLAEEPREQQELEALSQDPRRYEEWKWFRCPTLLEV
                     LEQFPSVALPAPLLLTQLPLLQPRYYSVSSAPSTHPGEIHLTVAVLAYRTQDGLGPLH
                     YGVCSTWLSQLKPGDPVPCFIRGAPSFRLPPDPSLPCILVGPGTGIAPFRGFWQERLH
                     DIESKGLQPTPMTLVFGCRCSQLDHLYRDEVQNAQQRGVFGRVLTAFSREPDNPKTYV
                     QDILRTELAAEVHRVLCLERGHMFVCGDVTMATNVLQTVQRILATEGDMELDEAGDVI
                     GVLRDQQRYHEDIFGLTLRTQEVTSRIRTQSFSLQERQLRGAVPWAFDPPGSDTNSP"
     polyA_site      4077
BASE COUNT      763 a   1371 c   1234 g    709 t
ORIGIN
        1 gaattcccac tctgctgcct gctccagcag acggacgcac agtaacatgg gcaacttgaa
       61 gagcgtggcc caggagcctg ggccaccctg cggcctgggg ctggggctgg gccttgggct
      121 gtgcggcaag cagggcccag ccaccccggc ccctgagccc agccgggccc cagcatccct
      181 actcccacca gcgccagaac acagcccccc gagctccccg ctaacccagc ccccagaggg
      241 gcccaagttc cctcgtgtga agaactggga ggtggggagc atcacctatg acaccctcag
      301 cgcccaggcg cagcaggatg ggccctgcac cccaagacgc tgcctgggct ccctggtatt
      361 tccacggaaa ctacagggcc ggccctcccc cggccccccg gcccctgagc agctgctgag
      421 tcaggcccgg gacttcatca accagtacta cagctccatt aagaggagcg gctcccaggc
      481 ccacgaacag cggcttcaag aggtggaagc cgaggtggca gccacaggca cctaccagct
      541 tagggagagc gagctggtgt tcggggctaa gcaggcctgg cgcaacgctc cccgctgcgt
      601 gggccggatc cagtggggga agctgcaggt gttcgatgcc cgggactgca ggtctgcaca
      661 ggaaatgttc acctacatct gcaaccacat caagtatgcc accaaccggg gcaaccttcg
      721 ctcggccatc acagtgttcc cgcagcgctg ccctggccga ggagacttcc gaatctggaa
      781 cagccagctg gtgcgctacg cgggctaccg gcagcaggac ggctctgtgc ggggggaccc
      841 agccaacgtg gagatcaccg agctctgcat tcagcacggc tggaccccag gaaacggtcg
      901 cttcgacgtg ctgcccctgc tgctgcaggc cccagatgag cccccagaac tcttccttct
      961 gccccccgag ctggtccttg aggtgcccct ggagcacccc acgctggagt ggtttgcagc
     1021 cctgggcctg cgctggtacg ccctcccggc agtgtccaac atgctgctgg aaattggggg
     1081 cctggagttc cccgcagccc ccttcagtgg ctggtacatg agcactgaga tcggcacgag
     1141 gaacctgtgt gaccctcacc gctacaacat cctggaggat gtggctgtct gcatggacct
     1201 ggatacccgg accacctcgt ccctgtggaa agacaaggca gcagtggaaa tcaacgtggc
     1261 cgtgctgcac agttaccagc tagccaaagt caccatcgtg gaccaccacg ccgccacggc
     1321 ctctttcatg aagcacctgg agaatgagca gaaggccagg gggggctgcc ctgcagactg
     1381 ggcctggatc gtgcccccca tctcgggcag cctcactcct gttttccatc aggagatggt
     1441 caactatttc ctgtccccgg ccttccgcta ccagccagac ccctggaagg ggagtgccgc
     1501 caagggcacc ggcatcacca ggaagaagac ctttaaagaa gtggccaacg ccgtgaagat
     1561 ctccgcctcg ctcatgggca cggtgatggc gaagcgagtg aaggcgacaa tcctgtatgg
     1621 ctccgagacc ggccgggccc agagctacgc acagcagctg gggagactct tccggaaggc
     1681 ttttgatccc cgggtcctgt gtatggatga gtatgacgtg gtgtccctcg aacacgagac
     1741 gctggtgctg gtggtaacca gcacatttgg gaatggggat cccccggaga atggagagag
     1801 ctttgcagct gccctgatgg agatgtccgg cccctacaac agctcccctc ggccggaaca
     1861 gcacaagagt tataagatcc gcttcaacag catctcctgc tcagacccac tggtgtcctc
     1921 ttggcggcgg aagaggaagg agtccagtaa cacagacagt gcaggggccc tgggcaccct
     1981 caggttctgt gtgttcgggc tcggctcccg ggcatacccc cacttctgcg cctttgctcg
     2041 tgccgtggac acacggctgg aggaactggg cggggagcgg ctgctgcagc tgggccaggg
     2101 cgacgagctg tgcggccagg aggaggcctt ccgaggctgg gcccaggctg ccttccaggc
     2161 cgcctgtgag accttctgtg tgggagagga tgccaaggcc gccgcccgag acatcttcag
     2221 ccccaaacgg agctggaagc gccagaggta ccggctgagc gcccaggccg agggcctgca
     2281 gttgctgcca ggtctgatcc acgtgcacag gcggaagatg ttccaggcta caatccgctc
     2341 agtggaaaac ctgcaaagca gcaagtccac gagggccacc atcctggtgc gcctggacac
     2401 cggaggccag gaggggctgc agtaccagcc gggggaccac ataggtgtct gcccgcccaa
     2461 ccggcccggc cttgtggagg cgctgctgag ccgcgtggag gacccgccgg cgcccactga
     2521 gcccgtggca gtagagcagc tggagaaggg cagccctggt ggccctcccc ccggctgggt
     2581 gcgggacccc cggctgcccc cgtgcacgct gcgccaggct ctcaccttct tcctggacat
     2641 cacctcccca cccagccctc agctcttgcg gctgctcagc accttggcag aagagcccag
     2701 ggaacagcag gagctggagg ccctcagcca ggatccccga cgctacgagg agtggaagtg
     2761 gttccgctgc cccacgctgc tggaggtgct ggagcagttc ccgtcggtgg cgctgcctgc
     2821 cccactgctc ctcacccagc tgcctctgct ccagccccgg tactactcag tcagctcggc
     2881 acccagcacc cacccaggag agatccacct cactgtagct gtgctggcat acaggactca
     2941 ggatgggctg ggccccctgc actatggagt ctgctccacg tggctaagcc agctcaagcc
     3001 cggagaccct gtgccctgct tcatccgggg ggctccctcc ttccggctgc cacccgatcc
     3061 cagcttgccc tgcatcctgg tgggtccagg cactggcatt gcccccttcc ggggattctg
     3121 gcaggagcgg ctgcatgaca ttgagagcaa agggctgcag cccactccca tgactttggt
     3181 gttcggctgc cgatgctccc aacttgacca tctctaccgc gacgaggtgc agaacgccca
     3241 gcagcgcggg gtgtttggcc gagtcctcac cgccttctcc cgggaacctg acaaccccaa
     3301 gacctacgtg caggacatcc tgaggacgga gctggctgcg gaggtgcacc gcgtgctgtg
     3361 cctcgagcgg ggccacatgt ttgtctgcgg cgatgttacc atggcaacca acgtcctgca
     3421 gaccgtgcag cgcatcctgg cgacggaggg cgacatggag ctggacgagg ccggcgacgt
     3481 catcggcgtg ctgcgggatc agcaacgcta ccacgaagac attttcgggc tcacgctgcg
     3541 cacccaggag gtgacaagcc gcatacgcac ccagagcttt tccttgcagg agcgtcagtt
     3601 gcggggcgca gtgccctggg cgttcgaccc tcccggctca gacaccaaca gcccctgaga
     3661 gccgcctggc tttcccttcc agttccggga gagcggctgc ccgactcagg tccgcccgac
     3721 caggatcagc cccgctcctc ccctcttgag gtggtgcctt ctcacatctg tccagaggct
     3781 gcaaggattc agcattattc ctccaggaag gagcaaaacg cctcttttcc ctctctaggc
     3841 ctgttgcctc gggcctgggt ccgccttaat ctggaaggcc cctcccagca gcggtacccc
     3901 agggcctact gccacccgct tcctgtttct tagtccgaat gttagattcc tcttgcctct
     3961 ctcaggagta tcttacctgt aaagtctaat ctctaaatca agtatttatt attgaagatt
     4021 taccataagg gactgtgcca gatgttagga gaactactaa agtgcctacc ccagctc
//