From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Klebsiella pneumoniae alpha galactosidase (melA) gene, 3' end and
Date: 21 Jul 92 10:06:43 GMT
Distribution: bionet
Checksum: 55154     5

LOCUS       KPNGALMEL    1624 bp ds-DNA             BCT       21-JUL-1992
DEFINITION  Klebsiella pneumoniae alpha galactosidase (melA) gene, 3' end and
            melibiose carrier (melB) gene, complete cds.
ACCESSION   M97257
KEYWORDS    alpha-galactosidase; melA gene; melB gene; melibiose carrier.
SOURCE      Klebsiella pneumoniae (individual_isolate 2002) DNA.
  ORGANISM  Klebsiella pneumoniae
            Prokaryotae; Gram-negative facultatively anaerobic rods;
            Enterobacteriaceae.
REFERENCE   1  (bases 1 to 1624)
  AUTHORS   Hama,H. and Wilson,T.
  TITLE     Primary structure and characteristics of the melibiose carrier of
            Klebsiella pneumoniae
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     CDS             1..106
                     /function="hydrolysis of alpha galactosides"
                     /product="alpha-galactosidase"
                     /gene="melA"
                     /evidence=EXPERIMENTAL
                     /codon_start=2
                     /translation="DPHTAAVLGIEEIYALVDDLIASHGDWLPAWLHR"
                     /partial
     RBS             193..195
                     /gene="melB"
     RBS             200..204
                     /gene="melB"
     CDS             200..1615
                     /function="cation-galactoside cotransport"
                     /product="melibiose carrier"
                     /gene="melB"
                     /evidence=EXPERIMENTAL
                     /codon_start=1
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDIVGLSVGVV
                     GTLFLVARILDAIADPIMGWIVNCTRSRWGKFKPWILIGTITNSVVLYMLFSAHHFSG
                     GALLAWVWLTYLLWGFTYTIMDVPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNAVGGADRGFGFQMFTLVLIAFFVVSTLVTLRNVHEVYSSDSGVSEDSSHL
                     SLRQMVALIYKNDQLACLLGMALAYNTAANIIAGFAIYYFTYVIGSAEMFPYYMSYAG
                     AANLLTLILFPRLVKGLSRRILWAGASIMPVLGCGVLLLMALGGVYNIALISLAGVLL
                     NIGTALFWVLQVIMVADTVDYGEYTMNIRCESIAYSVQTLVVKAGSAFAAWFIAIVLG
                     IIGYVPNVVQSSHTLLGMQAIMIALPTLFFALTLFLYFRYYKLNGDMLRRIQIHLLDK
                     YRRVPENDVEPERPIVVPNQV"
BASE COUNT      291 a    436 c    484 g    413 t
ORIGIN
        1 ggatccgcac accgccgcgg tgttggggat cgaagagatc tacgcgctgg tggacgatct
       61 gatcgccagc cacggcgact ggctgccggc ctggctgcac cgttaatcct cttatgccgc
      121 cgggggcaac gaacccggcg gtgcctgtca cgctctatca aacgctgaag gaggcgctgg
      181 cgacagcgac ctggacccta tgagtatttc aatgacaaca aagctcagct acggatttgg
      241 tgcctttggt aaagatttcg ctatcggtat cgtctacatg tacctgatgt attactacac
      301 cgatattgtt ggcttatcgg tcggggtggt tggcaccttg ttcctggtgg caaggatcct
      361 cgatgcgata gccgatccga ttatgggatg gatcgttaac tgtacgcggt cgcgatgggg
      421 aaagttcaaa ccgtggatcc tgatcggcac gataaccaac tcggtggtgc tgtatatgct
      481 gttcagcgcc caccatttca gcggcggggc gctgctggcg tgggtgtggc taacctacct
      541 gctgtggggc ttcacttaca ccatcatgga tgtgccgttc tggtcgttgg tgccgaccat
      601 taccctcgat aaacgcgaac gcgagcagct ggtgccttac ccgcgctttt tcgccagtct
      661 ggcgggattt gtcacggccg gcgtcacgct gccgttcgtc aacgccgtcg gcggcgccga
      721 tcgcggtttc ggttttcaga tgttcactct ggtgctgatc gccttcttcg tcgtttccac
      781 cctcgtcacc ctgcgcaacg tgcatgaggt ttactcctcc gacagtggtg tgagcgaaga
      841 ctccagccac ctctcactgc gccagatggt ggcgctgatc tataaaaacg atcagctggc
      901 ctgcctgtta ggcatggcgc tggcctataa cacggcggcg aacattatcg ctggctttgc
      961 gatttactac tttacctacg tgattggcag cgcggagatg ttcccgtatt acatgtccta
     1021 cgccggggcg gcgaatttgc tgacgctgat cctgttccca cgactggtaa aagggctatc
     1081 gcggcgcatt ctgtgggcgg gtgcttcgat catgccggtg ctgggctgcg gcgtgctgtt
     1141 gctgatggcg ctgggcggcg tttacaacat cgcgctgatt tcgctggccg gggtgctgtt
     1201 gaatatcggc accgcgctgt tctgggtgct gcaggttatt atggtggccg ataccgtcga
     1261 ctatggcgaa tatacgatga atatccgctg cgaaagcatc gcctattccg ttcaaacgct
     1321 ggtagtcaaa gccggttcgg cgtttgcggc ctggtttatc gccatcgtgc tgggtatcat
     1381 cggttacgtg ccgaatgttg tgcagtcgtc ccacacgctg ctggggatgc aggcgattat
     1441 gattgcgctg ccgacgctct tttttgctct gacgctgttt ctctatttcc gttattacaa
     1501 actgaatggc gatatgctgc gacggattca gattcacctg ctggataaat atcgccgggt
     1561 accggaaaat gacgtcgagc cggaacggcc gattgtggta cccaatcagg tgtaaaaagc
     1621 gtgc
//