From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: H.sapiens mRNA for amphiglycan
Date: 3 Jan 93 22:06:08 GMT
Distribution: bionet
Checksum: 38439 5

LOCUS       HUMAMPH      2613 bp ss-RNA             PRI       21-DEC-1992
DEFINITION  H.sapiens mRNA for amphiglycan
ACCESSION   X67016
KEYWORDS    amphiglycan; antithrombin III binding;
            heparan sulfate proteoglycan.
SOURCE      Homo sapiens RNA.
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chorqata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 2613)
  AUTHORS   David,G.S., van der Schueren,B., Marynen,P., Cassiman,J.J. and Van
            Den Berghe,H.
  TITLE     Molecular cloning of amphiglycan, a novel integral membrane heparan
            sulfate proteoglycan expressed by epithelial and fibroblastic cells
  JOURNAL   J. Cell Biol. 118, 961-969 (1992)
  STANDARD  full automatic
COMMENT     *source: strain=caucasian; *source: tissue=lung; *source:
            cell_type=fibroblast; *source: clone_library=lambda ZAP 11;
            *source: clone=35K17; *source: isolate=patient VDB;
               From EMBL    entry HSAMPH;  dated 01-DEC-1992.
FEATURES             Location/Qualifiers
     sig_peptide     27..80
     mat_peptide     81..620
                     /product="amphiglycan"
     polyA_signal    2575..2581
     polyA_site      2594
     CDS             27..623
                     /product="amphiglycan"
                     /codon_start=1
                     /translation="MAPARLFALLLLFVGGVAESIRETEVIDPQDLLEGRYFSGALPD
                     DEDVVGPGQESDDFELSGSGDLDDLEDSMIGPEVVHPLVPLDNHIPERAGSGSQVPTE
                     PKKLEENEVIPKRISPVEESEDVSNKVSMSSTVQGSNIFERTEVLAALIVGGIVGILF
                     AVFLILLLMYRMKKKDEGSYDLGKKPIYKKAPTNEFYA"
BASE COUNT      557 a    601 c    665 g    790 t
ORIGIN      
        1 gcgcgccttc tccagtccgc ggtgccatgg cccccgcccg tctgttcgcg ctgctgctgc
       61 tcttcgtagg cggagtcgcc gagtcgatcc gagagactga ggtcatcgac ccccaggacc
      121 tcctagaagg ccgatacttc tccggagccc taccagacga tgaggatgta gtggggcccg
      181 ggcaggaatc tgatgacttt gagctgtctg gctctggaga tctggatgac ttggaagact
      241 ccatgatcgg ccctgaagtt gtccatccct tggtgcctct agataaccat atccctgaga
      301 gggcagggtc tgggagccaa gtccccaccg aacccaagaa actagaggag aatgaggtta
      361 tccccaagag aatctcaccc gttgaagaga gtgaggatgt gtccaacaag gtgtcaatgt
      421 ccagcactgt gcagggcagc aacatctttg agagaacgga ggtcctggca gctctgattg
      481 tgggtggcat cgtgggcatc ctctttgccg tcttcctgat cctactgctc atgtaccgta
      541 tgaagaagaa ggatgaaggc agctatgacc tgggcaagaa acccatctac aagaaagccc
      601 ccaccaatga gttctacgcg tgaagcttgc ttgtgggcac tggcttggac tttagcgggg
      661 agggaagcca ggggattttg aagggtggac attagggtag ggtgaggtca acctaatact
      721 gacttgtcag tatctccagc tctgattacc tttgaagtgt tcagaagaga cattgtcttc
      781 tactgttctg ccaggttctt cttgagcttt gggcctcagt tgccctggca gaaaaatgga
      841 ttcaacttgg cctttctgaa ggcaagactg ggattggatc acttcttaaa cttccagtta
      901 agaatctagg tccgccctca agcccatact gaccatgcct catccagagc tcctctgaag
      961 ccagggggct aacggatgtt gtgtggagtc ctggctggag gtcctccccc agtggccttc
     1021 ctcccttcct ttcacagccg gtctctctgc caggaaatgg gggaaggaac tagaaccacc
     1081 tgcaccttga gatgtttctg taaatgggta cttgtgatca cactacggga atctctgtgg
     1141 tatatacctg gggccattct aggctctttc aagtgacttt tggaaatcaa ccttttttat
     1201 ttggggggga ggatggggaa aagagctgag agtttatgct gaaatggatt tatagaatat
     1261 ttgtaaatct atttttagtg tttgttcgtt tttttaactg ttcattcctt tgtgcagagt
     1321 gtatatctct gcctgggcaa gagtgtggag gtgccgaggt gtcttcattc tctcgcacat
     1381 ttccacagca cctgctaagt ttgtatttaa tggtttttgt ttttgttttt gtttgtttct
     1441 tgaaaatgag agaagagccg gagagatgat ttttattaat tttttttttt tttttttttt
     1501 tactatttat agctttagat agggcctccc ttcccctctt ctttctttgt tctctttcat
     1561 taaacccctt ccccagtttt ttttttatac tttaaacccc gctcctcatg gccttggccc
     1621 tttctgaagc tgcttcctct tataaaatag cttttgccga aacatagttt ttttttagca
     1681 gatcccaaaa tataatgaag gggatggtgg gatatttgtg tctgtgttct tataatatat
     1741 tattattctt ccttggttct agaaaaatag ataaatatat ttttttcagg aaatagtgtg
     1801 gtgtttccag tttgatgttg ctgggtggtt gagtgagtga attttcatgt ggctgggtgg
     1861 gtttttgcct ttttctcttg ccctgttcct ggtgccttct gatggggctg gaatagttga
     1921 ggtggatggt tctacccttt ctgccttctg tttgggaccc agctggtgtt ctttggtttg
     1981 ctttcttcag gctctagggc tgtgctatcc aatacagtaa ccacatgcgg ctgtttaaag
     2041 ttaagccaat taaaatcaca taagattaaa aattccttcc tcagttgcac taaccacgtt
     2101 tctagaggcg tcactgtatg tagttcatgg ctactgtact gacagcgaga gcatgtccat
     2161 ctgttggaca gcactattct agagaactaa actggcttaa cgagtcacag cctcagctgt
     2221 gctgggacga cccttgtctc cctgggtagg ggggggggaa tgggggaggg ctgatgaggc
     2281 cccagctggg gcctgttgtc tgggaccctc cctctcctga gaggggaggc ctggtggctt
     2341 agcctgggca ggtcgtgtct cctcctgacc ccagtggctg cggtgagggg aaccaccctc
     2401 ccttgctgca ccagtggcca ttagctcccg tcaccactgc aacccagggt cccagctggc
     2461 tgggtcctct tctgccccca gtgcccttcc ccttgggctg tgttggagtg agcacctcct
     2521 ctgtaggcac ctctcacact gttgtctgtt actgattttt tttgataaaa agataataaa
     2581 acctggtact ttctaaaaaa aaaaaaaaaa aaa
//