From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human profilaggrin gene.
Date: 29 Dec 92 00:45:36 GMT
Distribution: bionet
Checksum: 09898 9

LOCUS       HUMS100EF    4080 bp ds-DNA             PRI       03-DEC-1992
DEFINITION  Human profilaggrin gene.
ACCESSION   M90967
KEYWORDS    profilaggrin.
SOURCE      Homo sapiens male caucasian placenta DNA.
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 4080)
  AUTHORS   Presland,R.B., Fleckman,P., Haydock,P.V., Nirunsuksiri,W. and
            Dale,B.A.
  TITLE     Characterization of the human epidermal profilaggrin gene: analysis
            of gene structure and identification of an S-100-like calcium
            binding domain at the amino terminus
  JOURNAL   J. Biol. Chem. 267, 23772-23781 (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     intron          388..1715
                     /number=1
                     /label=intron1
                     /citation=[1]
                     /note="we have mapped the 3' splice junction by RNAse
                     protection,but are not certain about the 5' splice
                     junction. Therefore, the size of this intron is tentative
                     at present.; putative"
     misc_signal     1528..1535
                     /function="AP1 site"
                     /phenotype="acts to up-regulate transcription of genes by
                     fos/jun bindin"
                     /standard_name="AP1 site"
                     /label=AP1
                     /citation=[1]
                     /note="not known at present if this sequence binds
                     thec-fos/cjun/AP1 complex, or whether this sequence is
                     important for the upregulation of profilaggrin gene
                     expression by TPA; putative"
     exon            1716..1874
                     /number=2
                     /function="may bind one calcium ion"
                     /evidence="EXPERIMENTAL"
                     /label=exon2
                     /citation=[1]
     intron          1875..2444
                     /number=2
                     /evidence="EXPERIMENTAL"
                     /label=intron2
                     /citation=[1]
     exon            2445..4080
                     /number=3
                     /function="may bind calcium; aggregation of keratin IF
                     filaments"
                     /label=exon3
                     /citation=[1]
                     /partial
     misc_feature    2490..2525
                     /function="may bind calcium"
                     /standard_name="EF hand"
                     /citation=[1]
                     /partial
                     /note="S-100-like EF hand (calcium binding loop);
                     putative"
     CDS             <3186..>4080
                     /function="aggregates keratin IF filaments in epidermis"
                     /evidence="EXPERIMENTAL"
                     /citation=[1]
                     /note="portion of filaggrin-like coding region sequenced"
                     /codon_start=1
                     /translation="GSRVSQDRDSEGHSEDSERHSGSASRNHHGSAWEQSRDGSRHPR
                     SHDEDRASHGHSADSSRQSGTRHAETSSRGQTASSHEQARSSPGERHGSGHQQSADSS
                     RHSATGRGQASSAVSDRGHRGSSGSQASDSEGHSENSDTQSVSGHGKAGLRQQSHQES
                     TRGRSGERSGRSGSSLYQVSTHEQPDSAHGRTGTSTGGRQGSHHEQARDSSRHSASQE
                     GQDTIRGHPGSSRGGRQGSHHEQSVNRSGHSGSHHSHTTSQGRSDASHGQSGSRSASR
                     QTRNEEQSGDGTRHSGSRHHEA"
     CDS             1737..>1874
                     /function="encodes N-terminal S-100-like EF hand
                     domain;may bind Ca ion"
                     /citation=[1]
                     /note="putative"
                     /product="profilaggrin"
                     /codon_start=1
                     /translation="MSTLLENIFAIINLFKQYSKKDKNTDTLSKKELKELLEKEFRQI
                     LK"
BASE COUNT     1474 a    731 c    821 g   1054 t
ORIGIN      
        1 gaagggaatg atccagtaga caaagaagaa ttaatgatgc atatgagaaa gtaggcaact
       61 gcaaaagcaa agtgtttggg taggcaagag agagtggggc tcagtgccaa agtaaaggac
      121 aggaggagca cagactgttt attaccctaa ataaagtgaa ggcaaggtat atggatagaa
      181 atgtagtagg ttgggagatc agatattagg agaatgagga aactcttttc caatggcttc
      241 caagttctca ggaatcaaaa tcaaggacat cagagataaa ggaaagaaga agttgttgaa
      301 gctttggcca gtgggtataa gaaaaagtag gacaagtgga tgtatccaag gggcatttgg
      361 ggagattaaa cataatgtct aaccaaggta aaaagggaag tgagtacata aagcagtaaa
      421 tagagaggtt aaagaattat tgacatcagc gtacaagaga aggtcaaaga gtgagatcct
      481 tcagaaagag attttgcata tgttgcaagt aacgataagt tcagagaata caatatcatc
      541 ataaataaga ctggctgaaa ataatcataa actttaaaat gcatttgtaa gaagcatata
      601 cctgtaattt tttaaattca atctttacaa tctatatttt aactggcaag atatggtcta
      661 ttttacatgt attgtaatta gtaataataa aattatttgt aattctatta atttatttta
      721 tgctttctgt ttacccttct ttttctatgc ttcttatttt cttctttcct ggcttctgtt
      781 gaattattaa agtttcttaa ttcccctttt tcatctctaa tggtttagaa attataaatt
      841 ctattatttt agtaaatacc cataaactta cccaaataca tatatcactt aaccacagca
      901 aaattcatta atatctaatt cctcttacca aacaaaccaa ggtcaaaaat gttttagttg
      961 tgactgctac agtaatctcc ttcacattgt ttctacagta ttttagtttc acctggtttt
     1021 gtaagtccta ataaagttat aattctttat ataatacaca cttagattta ttgacatgct
     1081 tactaatttc ttcaccaatg agataaactg aacattcctt aaatggcaaa atgaaaccca
     1141 gtcagaaaaa cctttctctt taaatattga agagctatta aatccatcct atacaatgaa
     1201 atattataaa cttcatgaaa tgtttaaaaa acaaataaaa aattgaatga actttcattg
     1261 agtatcttgt catatggcta actggctttc agagaattgt gtcttagcaa attaatcatt
     1321 tattaagtta gcttttgggg aagtaatttt cagtaaattt gtataagtaa attttactgc
     1381 ttaaaaccag tcagcctttc taaattcttg ttgctagcaa tggcaagatc agcaagaggg
     1441 atgatcttac ctagagcaca ctgaggtctg tgagactact aagtccagct gtaagtggca
     1501 caaggccaaa aaatgggcaa ttttatttaa gtcaggaaaa ctctattcat ttgttttatg
     1561 tagatggtag agaaaagttt ctcatgtcca tcttccattc ttaaagatgg attacttaga
     1621 ggaaaatgta ggtttaaaat gtggaaaatg attgtaataa tataacctag aaaaaaagat
     1681 gtataaaaaa gtgcattttc atttcttctt tctaggttca catttattgc caaaagatgt
     1741 ctactctcct ggaaaacatc tttgccataa ttaatctttt caagcaatat tcaaaaaaag
     1801 ataaaaacac tgacacattg agtaaaaaag agctgaagga acttctggaa aaggaatttc
     1861 ggcaaatcct gaaggtaaga gtctctgaca agaccaaaga tagagcattt gtttttctca
     1921 tcaataatct aagcaagggt tattttgagc tctttgtgaa ctatttctct taaaatagga
     1981 aaagagttaa tcttgtctca aaagagatag attttatttt cttcccctat acctggatgt
     2041 atattataaa cataaagaca ccctcagaga aaaaaatata aatatttaaa aattaatgta
     2101 aattagttct tagtattttg gtatttataa tattctccca aacatcaaca ttactaaggg
     2161 gatttttgtt aaagacaact aagtaatgaa ttatatacac ttctgacttt taaaataaga
     2221 aaagagtcac ttaccccatc aaatcttgat accttactag tctacatctt tgttcacata
     2281 taacacctga atgaggatga gacaaaaaag tcttaaaaaa aaaaaagatt ttgtcccact
     2341 ttttaatact caaagatcag gttccttaaa ttaactttga attaattggc tgataatgtg
     2401 attctgtctg atgcagtctc cctctgtgac ttccctctgt acagaatcca gatgacccag
     2461 atatggttga tgtcttcatg gatcacttgg atatagacca caacaagaaa attgacttca
     2521 ctgagtttct tctgatggta ttcaagttgg ctcaagcata ttatgagtct accagaaaag
     2581 agaatttacc gatatcagga cacaagcaca gaaagcacag tcatcatgat aaacatgaag
     2641 ataataaaca ggaagaaaac aaagaaaaca gaaaaagacc ctcaagtctg gaaagaagaa
     2701 acaatagaaa agggaataag ggaagatcca agagcccaag agaaacaggg gggaaaaggc
     2761 atgaatctag ttctgaaaaa aaagaaagaa aaggatattc acctactcat agagaagaag
     2821 aatatggaaa aaaccatcat aactcaagta aaaaagagaa aaacaagact gaaaatacta
     2881 gattaggaga caataggaag aggctaagtg aaagacttga agagaaagaa gacaatgaag
     2941 aaggagtata tgattatgaa aatacaggaa gaatgactca aaaatggata caatcaggcc
     3001 atattgccac atattacaca atccaggatg aagcctatga caccactgat agtctattag
     3061 aagaaaacaa aatatatgaa agatcaaggt catctgatgg caaatcatca tctcaagtga
     3121 acaggtcaag acatgaaaat acaagccagg taccattgca ggagtccagg acaagaaagc
     3181 gtaggggatc cagagttagc caggacaggg acagtgaggg acactcagaa gactctgaga
     3241 ggcactctgg gtcggcttcc agaaaccatc atggatctgc gtgggagcag tcaagagatg
     3301 gctccagaca ccccaggtcc catgatgaag acagagccag tcatgggcac tctgcagaca
     3361 gctccagaca atcaggcact cgtcacgcag agacttcctc tcgtggacag actgcatcat
     3421 cccatgaaca ggcaagatca agtccaggag aaagacatgg atccggccac cagcagtcag
     3481 cagacagctc cagacactca gccactgggc gcgggcaagc ttcatctgca gtcagcgatc
     3541 gtggacaccg ggggtctagc ggtagtcagg ccagtgacag tgagggacat tcagaaaact
     3601 cagacacaca atcagtgtca ggccacggaa aggctgggct gagacagcag agccaccaag
     3661 agtccacacg tggccggtca ggggaacggt ctggacgttc agggtcttcc ctctaccagg
     3721 tgagcactca tgaacagcct gactctgccc atggacggac cgggaccagc actggaggaa
     3781 gacaaggatc gcaccacgag caggcacgag acagctccag gcattcagcg tcccaagagg
     3841 gtcaggacac cattcgtgga cacccggggt caagcagagg aggaaggcag ggatcccacc
     3901 acgagcaatc ggtaaatagg tctggacact caggttccca tcacagccac accacatccc
     3961 agggaaggtc tgatgcctcc catgggcagt caggatccag aagtgcaagc agacaaacac
     4021 gaaatgagga acaatcagga gacggcacca ggcactcagg gtcacgtcat catgaagctt
//