From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Aspergillus niger var awamori phytase gene, complete cds.
Date: 29 Dec 92 01:26:54 GMT
Distribution: bionet
Checksum: 42226 6

LOCUS       ASNPHYTAS    2379 bp ds-DNA             PLN       08-DEC-1992
DEFINITION  Aspergillus niger var awamori phytase gene, complete cds.
ACCESSION   L02421
KEYWORDS    phytase.
SOURCE      Aspergillus niger (strain ALK0243, sub_species awamori) DNA.
  ORGANISM  Aspergillus niger
            Eukaryota; Fungi; Eumycota; Ascomycotina; Plectomycetes;
            Eurotiales; Trichocomaceae.
REFERENCE   1  (sites)
  AUTHORS   Piddington,C.S., Houston,C.S., Paloheimo,M.T., Cantrell,M.A.,
            Miettinen-Oinonen,A., Nevalainen,H. and Rambosek,J.A.
  TITLE     The cloning and sequencing of the genes encoding phytase (phy) and
            pH 2.5 optimum acid phosphatase (aph) from Aspergillus niger var
            awamori
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
REFERENCE   2  (bases 1 to 2379)
  AUTHORS   Carter,J.R., Franden,M.A., Aebersold,R.H. and McHenry,C.S.
  TITLE     Molecular cloning, sequencing and overexpression of the gene
            encoding the psi subunit of E. coli DNA polymerase III holoenzyme
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     exon            420..463
                     /evidence="EXPERIMENTAL"
                     /citation=[2]
     exon            566..1925
                     /citation=[2]
                     /note="putative"
     CDS             join(420..463,566..1925)
                     /function="conversion of phytate to inositol and inorganic
                     phosphorus"
                     /EC_number="3.1.3.8"
                     /evidence="EXPERIMENTAL"
                     /citation=[2]
                     /product="phytase"
                     /codon_start=1
                     /translation="MGVSAVLLPLYLLAGVTSGLAVPASRNQSTCDTVDQGYQCFSET
                     SHLWGQYAPFFSLANESAISPDVPAGCRVTFAQVLSRHGARYPTESKGKKYSALIEEI
                     QQNVTTFDGKYAFLKTYNYSLGADDLTPFGEQELVNSGIKFYQRYESLTRNIIPFIRS
                     SGSSRVIASGEKFIEGFQSTKLKDPRAQPGQSSPKIDVVISEASSSNNTLDPGTCTVF
                     EDSELADTVEANFTATFAPSIRQRLENDLSGVTLTDTEVTYLMDMCSFDTISTSTVDT
                     KLSPFCDLFTHDEWIHYDYLQSLKKYYGHGAGNPLGPTQGVGYANELIARLTHSPVHD
                     DTSSNHTLDSNPATFPLNSTLYADFSHDNGIISILFALGLYNGTKPLSTTTVENITQT
                     DGFSSAWTVPFASRLYVEMMQCQAEQEPLVRVLVNDRVVPLHGCPIDALGRCTRDSFV
                     RGLSFARSGGDWAECSA"
BASE COUNT      561 a    735 c    518 g    565 t
ORIGIN      
        1 tgatggcggc ctagggcatc caggcaccct ttcccaacgg gggaacttcc gttgtccacg
       61 tgccctggtt cagccaatca aagcgtccca cggcaatgct ggatcaacga tcaacttgaa
      121 tgcaataaat gaagatgcaa ctaacaccat ctgttgcctt tctctcgaga aagctcctcc
      181 acttctcaca ctagatttat ccgttccttg tcgacttccc gtcccattcg gcctcgtcca
      241 ctgaagatct atcccaccat tgcacgtggg ccacctttgt gagcttctaa cctgaactgg
      301 tagagtatca cacaacatgc gaaagtggga tgaaggggtt atatgaggac cgtccggtcc
      361 ggcgcgatgg ccgtagctgc caatcgctgc tgtgcaagaa atttcttctc ataggcatca
      421 tgggcgtctc tgctgttcta cttcctttgt atctcctagc tgggtatgct aagcaccgct
      481 atctaagtct gataaggacc ctctttgccg agggcccctg aagctcggac tgtgtgggac
      541 tactgatcgc tgacaatctg tgcagagtca cctccggact ggcagtcccc gcctcgagaa
      601 atcaatccac ttgcgatacg gtcgatcaag ggtatcaatg cttctccgag acttcgcatc
      661 tttggggtca atacgcgccg ttcttctctc tggcaaacga atcggccatc tcccctgatg
      721 tgcccgccgg ttgcagagtc actttcgctc aggtcctctc ccgtcatgga gcgcggtatc
      781 cgaccgagtc caagggcaag aaatactccg ctctcattga ggagatccag cagaacgtga
      841 ccacctttga tggaaaatat gccttcctga agacatacaa ctacagcttg ggtgcagatg
      901 acctgactcc cttcggagag caggagctag tcaactccgg catcaagttc taccagcgat
      961 acgaatcgct cacaaggaac atcattccgt tcatccgatc ctctggctcc agccgcgtga
     1021 tcgcctccgg cgagaaattc attgagggct tccagagcac caagctgaag gatcctcgtg
     1081 cccagccggg ccaatcgtcg cccaagatcg acgtggtcat ttccgaggcc agctcatcca
     1141 acaacactct cgacccaggc acctgcactg tctttgaaga cagcgaattg gccgataccg
     1201 tcgaagccaa tttcaccgcc acgttcgccc cctccattcg tcaacgtctg gagaacgacc
     1261 tgtctggcgt gactctcaca gacacagaag tgacctacct catggacatg tgctccttcg
     1321 acaccatctc caccagcacc gtcgacacca agctgtcccc cttctgtgac ctgttcaccc
     1381 atgacgaatg gatccactac gactacctcc agtccctgaa aaaatactac ggccatggcg
     1441 caggtaaccc gctcggcccg acccagggcg tcggctacgc taacgagctc atcgcccgtc
     1501 tcacccactc gcctgtccac gatgacacca gctccaacca caccttggac tcgaacccag
     1561 ctaccttccc gctcaactct actctctacg cggacttttc ccacgataac ggcatcatct
     1621 ctatcctctt tgctttgggt ctgtacaacg gcactaagcc gctgtctacc acgaccgtgg
     1681 agaatatcac ccagacagat gggttctcgt ctgcttggac ggttccgttt gcttcgcgtc
     1741 tgtacgtcga gatgatgcag tgccaggccg agcaggagcc gctggtccgt gtcttggtta
     1801 atgatcgcgt tgtcccgctg catgggtgtc caattgatgc tttggggaga tgtacccggg
     1861 atagctttgt gagggggttg agctttgcta gatctggggg tgattgggcg gagtgttctg
     1921 cttagctgaa ctaccttgat ggatggtatg tatcaatcag agtacatatc attacttcat
     1981 gtatgtattt acgaagatgt acatatcgaa atatcgatga tgactactcc ggtagatatt
     2041 tggtcccctt ctatccttcg ttccacaacc atcgcactcg acgtacagca taatacaact
     2101 tcagcattaa caaacgaaca aataatatta tacactcctc cccaatgcaa taacaaccgc
     2161 aattcatacc tcatatagat acaatacaat acatccatcc ctaccctcaa gtccacccat
     2221 cccataatca aatccctact tactcctccc ccttcccaga acccaccccc gaaggagtaa
     2281 tagtagtagt agaagaagca gacgacctct ccaccaacct cttcggcctc ttatccccat
     2341 acgctataca cacacgaaca caccaaatag tcagcatgc
//