From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: E.coli codBA operon encoding cytosine permease and cytosine
Date: 15 Aug 92 10:01:35 GMT
Distribution: bionet
Checksum: 20295     7

LOCUS       ECOCODAB     3138 bp ds-DNA             BCT       15-AUG-1992
DEFINITION  E.coli codBA operon encoding cytosine permease and cytosine
            deaminase
ACCESSION   X63656
KEYWORDS    codA gene; codB gene; cytosine deaminase; cytosine permease.
SOURCE      Escherichia coli DNA.
  ORGANISM  Escherichia coli
            Prokaryotae; Gram-negative facultatively anaerobic rods;
            Enterobacteriaceae.
REFERENCE   1  (sites)
  AUTHORS   Danielsen,S., Kilstrup,M., Barilla,K., Jochimsen,B. and Neuhard,J.
  TITLE     Characterization of the Escherichia coli codBA operon encoding
            cytosine permease and cytosine deaminase
  JOURNAL   Unpublished (1992)
  STANDARD  full staff_review
REFERENCE   2  (bases 1 to 3138)
  AUTHORS   Neuhard,J.
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
COMMENT     *source: strain=K12;
            From EMBL    entry ECCODAB;  dated 21-FEB-1992.
FEATURES             Location/Qualifiers
     misc_feature    61..98
                     /note="REP sequence 1"
     misc_feature    104..146
                     /note="REP sequence 2"
     misc_feature    158..192
                     /note="REP sequence 3"
     misc_feature    204..240
                     /note="REP sequence 4"
     misc_signal     312..324
                     /note="PurBox"
     promoter        321..348
                     /note="sigma70 promoter"
     CDS             393..1652
                     /product="cytosine permease"
                     /gene="codB"
                     /codon_start=1
                     /translation="MSQDNNFSQGPVPQSARKGVLALTFVMLGLTFFSASMWTGGTLG
                     TGLSYHDFFLAVLIGNLLLGIYTSFLGYIGAKTGLTTHLLARFSFGVKGSWLPSLLLG
                     GTQVGWFGVGVAMFAIPVGKATGLDINLLIAVSGLLMTVTVFFGISALTVLSVIAVPA
                     IACLGGYSVWLAVNGMGGLDALKAVVPAQPLDFNVALALVVGSFISAGTLTADFVRFG
                     RNAKLAVLVAMVAFFLGNSLMFIFGAAGAAALGMADISDVMIAQGLLLPAIVVLGLNI
                     WTTNDNALYASGLGFANITGMSSKTLSVINGIIGTVCALWLYNNFVGWLTFLSAAIPP
                     VGGVIIADYLMNRRRYEHFATTRMMSVNWVAILAVALGIAAGHWLPGIVPVNAVLGGA
                     LSYLILNPILNRKTTAAMTHVEANSVE"
     CDS             1642..2925
                     /product="cytosine deaminase"
                     /gene="codA"
                     /codon_start=1
                     /translation="MSNNALQTIINARLPGEEGLWQIHLQDGKISAIDAQSGVMPITE
                     NSLDAEQGLVIPPFVEPHIHLDTTQTAGQPNWNQSGTLFEGIERWAERKALLTHDDVK
                     QRAWQTLKWQIANGIQHVRTHVDVSDATLTALKAMLEVKQEVAPWIDLQIVAFPQEGI
                     LSYPNGEALLEEALRLGADVVGAIPHFEFTREYGVESLHKTFALAQKYDRLIDVHCDE
                     IDDEQSRFVETVAALAHHEGMGARVTASHTTAMHSYNGAYTSRLFRLLKMSGINFVAN
                     PLVNIHLQGRFDTYPKRRGITRVKEMLESGINVCFGHDDVFDPWYPLGTANMLQVLHM
                     GLHVCQLMGYGQINDGLNLITHHSARTLNLQDYGIAAGNSANLIILPAENGFDALRRQ
                     VPVRYSVRGGKVIASTQPAQTTVYLEQPEAIDYKR"
     misc_feature    2954..2988
                     /note="REP sequence 5"
     misc_feature    3004..3034
                     /note="REP sequence 6"
BASE COUNT      666 a    805 c    871 g    796 t
ORIGIN
        1 gatctgacga ccattgatga tcctgcgtcg ttggatcaga tccgccaggc gatggaagag
       61 taggttattg tcggatgcgt cgcgcggtgc atccggcact gtgtgccgat gcctgatgcg
      121 acgctgacgc gttttatcat gcctacggac ctgaaccgta ggtcggataa ggcgctcgcg
      181 tcgcatccga caccatgctc agatgcctga tgcgacgctg acgcgtctta tcaggcctac
      241 ccactgtttt tacaccgata atttttcccc cacctttttg cactcattca tataaaaaat
      301 atatttcccc acgaaaacga ttgcttttta tcttcagatg aatagaatgc ggcggatttt
      361 ttgggtttca aacagcaaaa agggggaatt tcgtgtcgca agataacaac tttagccagg
      421 ggccagtccc gcagtcggcg cggaaagggg tattggcatt gacgttcgtc atgctgggat
      481 taaccttctt ttccgccagt atgtggaccg gcggcactct cggaaccggt cttagctatc
      541 atgatttctt cctcgcagtt ctcatcggta atcttctcct cggtatttac acttcatttc
      601 tcggttacat tggcgcaaaa accggcctga ccactcatct tcttgctcgc ttctcgtttg
      661 gtgttaaagg ctcatggctg ccttcactgc tactgggcgg aactcaggtt ggctggtttg
      721 gcgtcggtgt ggcgatgttt gccattccgg tgggtaaggc aaccgggctg gatattaatt
      781 tgctgattgc cgtttccggt ttactgatga ccgtcaccgt cttttttggc atttcggcgc
      841 tgacggttct ttcggtgatt gcggttccgg ctatcgcctg cctgggcggt tattccgtgt
      901 ggctggctgt taacggcatg ggcggcctgg acgcattaaa agcggtcgtt cccgcacaac
      961 cgttagattt caatgtcgcg ctggcgctgg ttgtggggtc atttatcagt gcgggtacgc
     1021 tcaccgctga ctttgtccgg tttggtcgca atgccaaact ggcggtgctg gtggcgatgg
     1081 tggccttttt cctcggcaac tcgttgatgt ttattttcgg tgcagcgggc gctgcggcac
     1141 tgggcatggc ggatatctct gatgtgatga ttgctcaggg cctgctgctg cctgcgattg
     1201 tggtgctggg gctgaatatc tggaccacca acgataacgc actctatgcg tcgggtttag
     1261 gtttcgccaa cattaccggg atgtcgagca aaaccctttc ggtaatcaac ggtattatcg
     1321 gtacggtctg cgcattatgg ctgtataaca attttgtcgg ctggttgacc ttcctttcgg
     1381 cagctattcc tccagtgggt ggcgtgatca tcgccgacta tctgatgaac cgtcgccgct
     1441 atgagcactt tgcgaccacg cgtatgatga gtgtcaattg ggtggcgatt ctggcggtcg
     1501 ccttggggat tgctgcaggc cactggttac cgggaattgt tccggtcaac gcggtattag
     1561 gtggcgcgct gagctatctg atccttaacc cgattttgaa tcgtaaaacg acagcagcaa
     1621 tgacgcatgt ggaggctaac agtgtcgaat aacgctttac aaacaattat taacgcccgg
     1681 ttaccaggcg aagaggggct gtggcagatt catctgcagg acggaaaaat cagcgccatt
     1741 gatgcgcaat ccggcgtgat gcccataact gaaaacagcc tggatgccga acaaggttta
     1801 gttataccgc cgtttgtgga gccacatatt cacctggaca ccacgcaaac cgccggacaa
     1861 ccgaactgga atcagtccgg cacgctgttt gaaggcattg aacgctgggc cgagcgcaaa
     1921 gcgttattaa cccatgacga tgtgaaacaa cgcgcatggc aaacgctgaa atggcagatt
     1981 gccaacggca ttcagcatgt gcgtacccat gtcgatgttt cggatgcaac gctaactgcg
     2041 ctgaaagcaa tgctggaagt gaagcaggaa gtcgcgccgt ggattgatct gcaaatcgtc
     2101 gccttccctc aggaagggat tttgtcgtat cccaacggtg aagcgttgct ggaagaggcg
     2161 ttacgcttag gggcagatgt agtgggggcg attccgcatt ttgaatttac ccgtgaatac
     2221 ggcgtggagt cgctgcataa aaccttcgcc ctggcgcaaa aatacgaccg tctcatcgac
     2281 gttcactgtg atgagatcga tgacgagcag tcgcgctttg tcgaaaccgt tgctgccctg
     2341 gcgcaccatg aaggcatggg cgcgcgagtc accgccagcc acaccacggc aatgcactcc
     2401 tataacgggg cgtatacctc acgcctgttc cgcttgctga aaatgtccgg tattaacttt
     2461 gtcgccaacc cgctggtcaa tattcatctg caaggacgtt tcgatacgta tccaaaacgt
     2521 cgcggcatca cgcgcgttaa agagatgctg gagtccggca ttaacgtctg ctttggtcac
     2581 gatgatgtct tcgatccgtg gtatccgctg ggaacggcga atatgctgca agtgctgcat
     2641 atggggctgc atgtttgcca gttgatgggc tacgggcaga ttaacgatgg cctgaattta
     2701 atcacccacc acagcgcaag gacgttgaat ttgcaggatt acggcattgc cgccggaaac
     2761 agcgccaacc tgattatcct gccggctgaa aatgggtttg atgcgctgcg ccgtcaggtt
     2821 ccggtacgtt attcggtacg tggcggcaag gtgattgcca gcacacaacc ggcacaaacc
     2881 accgtatatc tggagcagcc agaagccatc gattacaaac gttgaacgac tgggttacag
     2941 cgagcttagt ttatgccgga tgcgcggtga acgccttatc cggcctacgt agagcactga
     3001 actcgtaggc ctgataagcg tagcgcatca ggcaattcca gccgctgatc tgtgtcagcg
     3061 gctaccgtga ttcattcccg ccaacaaccg cgcattcctc caacgccatg tgcaaaaatg
     3121 ccttcgcagc ggctgtct
//