From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: S.typhimurium genes for branched-chain amino acid permerases,
Date: 12 Jan 93 05:50:53 GMT
Distribution: bionet
Checksum: 54545 10

LOCUS       STYLIV       4072 bp ds-DNA             BCT       12-JAN-1993
DEFINITION  S.typhimurium genes for branched-chain amino acid permerases,
            complete cds.
ACCESSION   D12589 D01215
KEYWORDS    branched-chain amino acid permease;
            branched-chain amino acid transport protein; membrane protein.
SOURCE      Salmonella typhimurium (strain LT2) DNA.
  ORGANISM  Salmonella typhimurium
            Prokaryotae; Bacteria; Gracilicutes; Scotobacteria; Facultatively
            anaerobic rods; Enterobacteriaceae.
REFERENCE   1  (sites)
  AUTHORS   Ohnishi,K., Nakazima,A., Matsubara,K. and Kiritani,K.
  TITLE     Cloning and nucleotide sequence of livB and livC, the structural
            genes encoding proteins of the high-affinity branched-chain amino
            acid transport in Salmonella typhimurium
  JOURNAL   J. Biochem. 107, 202-208 (1990)
  STANDARD  full automatic
REFERENCE   2  (bases 1 to 4072)
  AUTHORS   Matsubara,K., Ohnishi,K. and Kiritani,K.
  TITLE     Nucleotide sequences and characterization of liv genes encoding
            components of the high-affinity branched-chain amino acid transport
            system in Salmonella typhimurium
  JOURNAL   J. Biochem. 112, 93-101 (1992)
  STANDARD  full automatic
COMMENT     Submitted (17-Oct-1991) to DDBJ by: Kuniharu Ohnishi Department of
            Microbiology School of Pharmacy, Hokuriku University Ho-3
            Kanagawa-machi Kanazawa 920-11 Japan Phone:  0762-29-1161 x348 Fax:
               0762-29-1393.
FEATURES             Location/Qualifiers
     attenuator      26..44
                     /note="transcription attenuation dyad"
     terminator      3743..3792
                     /note="transcription termination dyad"
     CDS             63..989
                     /gene="LivA"
                     /product="LivA protein"
                     /codon_start=1
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTSWLLVAAGFIGAIIIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWIVGSSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYGVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGVAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     CDS             986..2263
                     /gene="LivE"
                     /product="LivE protein"
                     /codon_start=1
                     /translation="MKPMHIAMALFSAAMFFVLAGVFMGVQLELDGTKLVVDTAADIR
                     WQWIFIGTAVVFFFQLLRPMFQKAVKHVSGPKFILPAIDGSTVKQKLFLMALLVIAVA
                     WPFMVSRGSVDIATMTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPLAGLVSAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTLFGLEFSRSTREGGWDTFSNFFGVKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPTRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAVLLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKSGQAKGEQA"
     CDS             2260..3027
                     /gene="LivF"
                     /product="LivF protein"
                     /codon_start=1
                     /translation="MSQPLLAVNGLMMRFGGLLAVNNVSLELREREIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTITLRERHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPAFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEEIRNNPDVIRAYLGEA"
     CDS             3029..3742
                     /gene="LivG"
                     /product="LivG protein"
                     /codon_start=1
                     /translation="MEKTMLTFEKVSAHYGKIQALHDVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRASSGRVVFDGKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDRFQERIKWVYELFPRLHERRIQRAGTMSGGEQQMLAIGRALMSQPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
BASE COUNT      779 a   1071 c   1267 g    955 t
ORIGIN      Map position 76 min.
        1 taatgccaga agataagtta tcccaccgcc cgcacgccgg gcgggtttaa aaaggttacc
       61 ttatgtccga gcagtttctc tatttcttgc agcagatgtt taacggcgtc acgctgggga
      121 gcacctacgc gctgattgct atcggttata cgatggtgta cggcattatc ggcatgatca
      181 acttcgctca tggcgaggtg tatatgatcg gcagctacgt ctcttttatg atcatcgccg
      241 cgctaatgat gatgggaata gacaccagtt ggctcctggt ggccgctgga tttatcggtg
      301 cgattattat tgccagcgcc tacggctgga gtatcgagcg agtcgcttac cggcccgtgc
      361 gcaactccaa gcgcctgatt gccctgattt ccgccatcgg gatgtccatc ttcctgcaaa
      421 actacgtcag cctgacggaa ggttcgcgcg acgtggcgtt acccagcctg tttaacggcc
      481 agtggattgt gggcagcagc gaaaactttt ccgcgtccat caccaccatg caggcggtta
      541 tctggatagt cacctttctg gctatgctgg cgctcaccat tttcattcgc tactcccgga
      601 tgggccgcgc ctgccgcgcc tgcgcggagg atctgaaaat ggccagcctg ctcggcatta
      661 ataccgaccg cgtcattgcg ctaacgtttg ttatcggcgc ggcaatggcg gcggtggcgg
      721 gcgtgctgct cggtcagttc tatggcgtaa ttaaccccta catcggtttt atggccggga
      781 tgaaagcgtt caccgcggcg gtgctgggcg ggattggcag cattccgggc gcgatgatcg
      841 gcggtctgat tttgggcgtc gccgaagcgc tctcgtctgc atatttaagt accgaatata
      901 aagatgtcgt atcgttcgct ctgctgattc tggtactgct ggtgatgccg accggtatcc
      961 tggggcgtcc ggaggtagag aaagtatgaa accgatgcat attgcgatgg ctctgttctc
     1021 tgcggcgatg ttcttcgttc tggctggcgt cttcatgggc gtacaactgg agctggacgg
     1081 caccaaactg gtggtggata ccgccgccga catccgctgg cagtggatct ttatcggtac
     1141 ggcagtggtt tttttctttc agttgctgcg tccgatgttc cagaaagcgg tgaagcacgt
     1201 ctccggaccg aagtttattc tgccggcgat cgatggctct accgttaagc aaaagctgtt
     1261 tctgatggcg ctgctggtta tcgccgtggc atggccgttt atggtgtcgc gcggtagcgt
     1321 cgatatcgcc accatgacca tgatttatat catcctcggt ctggggctga acgtggtggt
     1381 ggggctgtcc ggtctgctgg tattgggcta cggcggcttt tacgccatcg gcgcttatac
     1441 ctttgcgctg ctcaaccact attatggttt gggtttctgg acctgcctgc cgctggcggg
     1501 actggtctcc gccgctgctg gtttcctgct cggcttcccg gtactgcgtc tgcgcggcga
     1561 ctatctggcg attgtgacgc tcggcttcgg cgaaatcgtc cgtatcctgc tgctcaacaa
     1621 cactgaaatt accggcggcc cgaacggcat cagccagatc ccgaaaccga cgctgtttgg
     1681 tctggagttt agccgcagca cccgcgaagg cggctgggat accttcagca atttctttgg
     1741 cgtgaagtac gacccatccg accgggtcat tttcctctat ctggtggccc tgttgctggt
     1801 ggtcctgtcg ctgtttgtca ttaaccgcct gctgcgtatg ccgctgggcc gggcgtggga
     1861 agcgttgcgt gaagatgaga tcgcctgtcg ctcattaggt ttaagcccga cgcgcatcaa
     1921 gctgaccgct tttactatca gcgccgcgtt tgccggtttc gccgggacgt tgtttgccgc
     1981 ccgccagggc tttgtcagcc cggaatcctt cactttcgcc gaatccgcct tcgtgctggc
     2041 gattgtggtg ctgggcggaa tgggctcgca gtttgcggtg atcctggcgg cggttctgct
     2101 ggtggtgtcg cgcgagttga tgcgcgactt caacgaatac agcatgttga tgctcggcgg
     2161 tttgatggtg ttgatgatga tctggcgccc gcagggattg ctgccgatga cgcgtccgca
     2221 gttgaagctg aaaagtggtc aggcgaaagg agagcaggca tgagtcagcc attattagcg
     2281 gttaacggcc tgatgatgcg ctttggcggt ttgctggcgg tcaacaatgt atcgctggaa
     2341 ttgcgtgagc gggaaattgt gtcgctgatt ggccctaacg gcgcgggtaa aacgacggtg
     2401 tttaactgcc tgaccggttt ttataagccg acaggcggta cgatcacgtt gcgcgagcgg
     2461 catctggaag gattgccggg ccagcagatc gcccgcatgg gcgtggtacg cactttccag
     2521 catgtgcgcc tgtttcgcga aatgacggtg attgaaaatc tgctggtggc gcagcaccag
     2581 caactgaaaa cgggcctctt ttccggcctg ttgaaaacgc ccgctttccg ccgcgcgcaa
     2641 agcgaagcgc tggaccgcgc ggcgacctgg cttgagcgta ttggcctgct ggaacatgcc
     2701 aaccgccagg ccagtaatct ggcctacggc gaccagcgcc ggctggagat tgcccgctgc
     2761 atggtgacgc agccagaaat tctgatgctc gacgaaccgg cggctggcct taacccgaaa
     2821 gagaccaaag agctggatga gctgattgcc gaactacgta accatcacaa caccactatc
     2881 ttgctgattg aacacgatat gaagctggtg atgggcattt ccgatcgcat ttacgtggtg
     2941 aaccagggca cgccgctggc gaacggcacg ccggaagaga tccgcaacaa cccggacgtc
     3001 atccgcgctt atctgggtga ggcataagat ggaaaaaacg atgttaacgt ttgaaaaagt
     3061 cagcgcccac tacggcaaga ttcaggcgct gcacgacgtc agtctgcaca tcaaccaggg
     3121 ggaaatcgtt accctgattg gcgctaacgg cgcggggaaa acgacgctgc ttggcacctt
     3181 gtgcggcgat ccgcgcgcct ccagcggacg ggtggtgttt gacggtaaag acataaccga
     3241 ctggcagacg gcgaaaatca tgcgtgaagc ggtggcgata gtgccggaag ggcggcgcgt
     3301 cttctcgcgg atgacggtag aagagaacct ggcgatgggc ggtttttttg ccgagcgcga
     3361 tcggtttcag gagcgtatca aatgggtgta cgagctgttt ccgcgcctgc atgagcgccg
     3421 tatccagcga gcgggcacca tgtccggcgg cgaacagcag atgctggcga ttggccgcgc
     3481 gctgatgagc cagccgaggc tattgctgct ggatgaaccg tcgctggggc tggcgccgat
     3541 catcatccag cagatcttcg acaccattga gcagttgcgc gagcagggta tgaccatctt
     3601 ccttgtcgag cagaacgcca accaggcgct gaagctcgcc gaccgcggtt atgtgctgga
     3661 aaacggccac gtggtgctat ccgataccgg tgatgcactg ctggcgaacg aagcggtgcg
     3721 cagcgcctat ctgggcggtt agactgtgcc aggggggaag cgcgtgcgcc gcccccggta
     3781 acggcaagcg gtaaaatttt tcaaaagagc ctgatggctc tttttttgta catgtttgtt
     3841 gtacaataaa catgtacaac tggggagatg tttatgcgta cggttaacta tagcgaagcg
     3901 cggcaaaatc tggccgaagt cctggaaagt gcggtgacgg gggggcctgt taccatcacg
     3961 cgtcgtgggc ataagtccgc agtgatcatc agcgccgagg agtttgagcg ttatcagacg
     4021 gcgcgaatgg atgatgagtt tgctgccatt atggcggttc atggcaatga gc
//