From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Simian immunodeficiency virus (32H reisolate of SIVmac251 from
Date: 26 Jan 93 05:51:25 GMT
Distribution: bionet
Checksum: 63528 6

LOCUS       SIV22515MO   1122 bp ss-RNA             VRL       25-JAN-1993
DEFINITION  Simian immunodeficiency virus (32H reisolate of SIVmac251 from
            I225) gp120 (V2, V4) RNA, partial cds's.
ACCESSION   M92738
KEYWORDS    .
SOURCE      Simian immunodeficiency virus 15 months post infection cDNA to
            genomic RNA.
  ORGANISM  Simian immunodeficiency virus
            Viridae; ss-RNA enveloped viruses; Positive strand RNA virus;
            Retroviridae.
REFERENCE   1  (bases 1 to 1122)
  AUTHORS   Almond,N.M., Jenkins,A.R., Slade,A., Heath,A.B., Cranage,M. and
            Kitchin,P.A.
  TITLE     Population sequence analysis of a simian immunodeficiency virus
            (32H re-isolate of SIVmac251): A virus stock used for international
            vaccine studies
  JOURNAL   AIDS Res. Hum. Retroviruses 8, 77-88 (1992)
  STANDARD  full automatic
REFERENCE   2  (bases 1 to 1122)
  AUTHORS   Almond,N.M., Jenkins,A.R., Heath,A.B., Taffs,F. and Kitchin,P.A.
  TITLE     The genetic evolution of the envelope gene of simian
            immunodeficiency virus in cynomolgus macaques infected with a
            complex virus pool
  JOURNAL   Virology 191, 996-1002 (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     variation       replace(123,"")
                     /citation=[2]
                     /note="'a' in variation"
     misc_feature    795..1121
                     /partial
                     /note="The sequence of this region of the PCR product
                     wasdetermined from the analysis of clones derived from the
                     PCRproduct"
     variation       replace(841..849,"")
                     /frequency=0.25
                     /citation=[1]
                     /note="'' in variation"
     variation       replace(841,"")
                     /frequency=0.375
                     /citation=[1]
                     /note="'t' in variation"
     variation       replace(842,"")
                     /frequency=0.375
                     /citation=[1]
                     /note="'c' in variation"
     variation       replace(845,"")
                     /frequency=0.375
                     /citation=[1]
                     /note="'g' in variation"
     CDS             1..1122
                     /note="Partial sequence analysis of the PCR product of SIV
                     gp120obtained by direct sequence analysis. The region of
                     sequenceextends from the end of V1 to the gp120:gp41
                     junction"
                     /product="gp120"
                     /codon_start=1
                     /translation="KIDMVNETSSCIAQNNCTGLEQEQMISCKFNMTGLKRDKKKEYN
                     ETWYSTDLVCEQGNSTDNESRCYMNHCNTSVIQESCDKHYWDTIRFRYCAPPGYALLR
                     CNDTNYSGFMPKCSKVVVSSCTRMMETQTSTWFGFNGTRAENRTYIYWHGRDNRTIIS
                     LNKYYNLTIKCRRPGNKTVLPVTIMSGLVFHSQPINDRPKQAWCWFGGDWKDAIKEVK
                     QTIVKHPRYTGTNNTDKINLTAPGGGDPEVTFMWTNCRGEFLYCKMNWFLNWVEDRNI
                     TNQKLKEQHKRNYVPCHIRQIINTWHKVGKNVYLPPREGDLTCNSTVTSLIANIDWID
                     GSQTNITMSAEVAELYRLELGDYKLVEITPIGLAPTDVKR"
     CDS             49..277
                     /gene="V2"
                     /partial
                     /product="gp120"
                     /codon_start=1
                     /translation="CTGLEQEQMISCKFNMTGLKRDKKKEYNETWYSTDLVCEQGNST
                     DNESRCYMNHCNTSVIQESCDKHYWDTIRFRY"
     CDS             784..874
                     /gene="V4"
                     /partial
                     /product="gp120"
                     /codon_start=1
                     /translation="CKMNWFLNWVEDRNITNQKLKEQHKRNYVP"
BASE COUNT      398 a    174 c    253 g    297 t
ORIGIN      
        1 aaaatagaca tggtcaatga gactagttct tgtatagctc agaataattg cacaggcttg
       61 gaacaagagc aaatgataag ctgtaaattc aacatgacag ggttaaaaag agacaagaaa
      121 aaggagtaca atgaaacttg gtactctaca gatttggttt gtgaacaagg gaatagcact
      181 gataatgaaa gtagatgcta catgaatcac tgtaacactt ctgttatcca agagtcttgt
      241 gacaaacatt attgggatac tattagattt aggtattgtg cacctccagg ttatgctttg
      301 cttagatgta atgacacaaa ttattcaggc tttatgccta aatgttctaa ggtggtggtc
      361 tcttcatgca caaggatgat ggagacacag acttctactt ggtttggctt taatggaact
      421 agagcagaaa atagaactta tatttactgg catggtaggg ataataggac tataattagt
      481 ttaaataagt attataatct aacaataaaa tgtagaagac caggaaataa gacagtttta
      541 ccagtcacca ttatgtctgg attggttttc cactcacaac caatcaatga taggccaaag
      601 caggcatggt gttggtttgg aggagattgg aaggatgcaa taaaagaggt gaagcagacc
      661 attgtcaaac atcccaggta tactggaact aacaatactg ataaaatcaa tttgacggct
      721 cctggaggag gagatccgga agttaccttc atgtggacaa attgcagagg agagttcctc
      781 tactgtaaaa tgaattggtt tctaaattgg gtagaagata ggaatataac taaccagaag
      841 ctaaaggaac agcataaaag gaattacgtg ccatgtcata ttagacaaat aatcaacact
      901 tggcataaag taggaaaaaa tgtttatttg cctccaagag agggagacct cacgtgtaac
      961 tccacagtga ccagtctcat agcaaacata gattggattg atggaagtca aactaatatc
     1021 accatgagtg cagaggtggc agaactgtat cgattggaat tgggagatta taaattagta
     1081 gagatcactc caattggctt ggcccccaca gatgtgaaga gg
//