From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: B. megaterium glucose dehydrogenase (GlcDH-IV) gene, complete cds.
Date: 5 Nov 92 23:05:14 GMT
Distribution: bionet
Checksum: 24613 4

LOCUS       BACGDH4      1123 bp ds-DNA             BCT       17-OCT-1992
DEFINITION  B. megaterium glucose dehydrogenase (GlcDH-IV) gene, complete cds.
ACCESSION   D10626
KEYWORDS    glucose dehydrogenase.
SOURCE      Bacillus megaterium (strain IAM1030) DNA.
  ORGANISM  Bacillus megaterium
            Prokaryotae; Firmicutes; Endospore-forming rods and cocci;
            Bacillaceae.
REFERENCE   1  (bases 1 to 1123)
  AUTHORS   Mitamura,T.
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
REFERENCE   2  (sites)
  AUTHORS   Nagao,T., Mitamura,T., Wang,X., Negoro,S., Yomo,T., Urabe,T. and
            Okada,H.
  TITLE     Cloning, nucleotide sequence, and enzymatic properties of glucose
            dehydrogenase isozymes from Bacillus megaterium IAM1030
  JOURNAL   J. Bacteriol. 174, 5013-5020 (1992)
  STANDARD  full automatic
COMMENT     Submitted (28-FEB-1992) to DDBJ by: Toshihide Mitamura Department
            of Biotechnology Faculty of Engineering Osaka University 2-1
            Yamada-oka, Suita-shi Osaka 565 Japan Phone:  06-877-5111 x4373
            Fax:    06-876-2250

               [2]  sites.
FEATURES             Location/Qualifiers
     -35_signal      154..159
     -10_signal      177..182
     RBS             206..211
     CDS             220..1005
                     /EC_number="1.1.1.47"
                     /gene="gdhIV"
                     /product="glucose dehydrogenase"
                     /codon_start=1
                     /translation="MYTDLKDKVVVITGGSTGLGRAMAVRFGQEEAKVVINYYNNEEE
                     ALDAKKEVEEAGGQAIIVQGDVTKEEDVVNLVQTAIKEFGTLDVMINNAGVENPVPSH
                     ELSLDNWNKVIDTNLTGAFLGSREAIKYFVENDIKGNVINMSSVHEMIPWPLFVHYAA
                     SKGGMKLMTETLALEYAPKGIRVNNIGPGAMNTPINAEKFADPVQRADVESMIPMGYI
                     GKPEEVAAVAAFLASSQASYVTGITLFADGGMTKYPSFQAGRG"
BASE COUNT      384 a    191 c    243 g    305 t
ORIGIN      
        1 aagcttctca ttatcacaaa tgggaatcgt tatttcaacg cttggcggta tcttcttact
       61 tggagaaaag aaaacaaagc gtcagcttat ttcaattacg attggtattg tgctcatcat
      121 tttaggtggc gtattcctcg gaatggctaa aagttaacgt ttacgtaaaa gttaatgata
      181 gtatgtatga ataaatgaaa acactaggag gattttatta tgtatacaga tttaaaagat
      241 aaagtagttg taattacagg tggatcaaca ggtttaggac gtgcaatggc tgttcgtttc
      301 ggtcaagaag aagcaaaagt tgttattaac tattacaaca atgaagaaga agctttagat
      361 gcgaaaaaag aagtagaaga agcaggcgga caagcaatca tcgttcaagg cgacgtaaca
      421 aaagaagaag acgttgtaaa ccttgttcaa acagctatta aagaattcgg aacattagac
      481 gttatgatta ataacgctgg tgttgaaaac ccagttcctt ctcatgagct atctttagac
      541 aactggaaca aagttattga tacaaactta acaggtgcat tcttaggaag ccgtgaagca
      601 attaaatatt tcgttgaaaa tgacattaaa ggaaacgtta ttaacatgtc cagcgttcac
      661 gaaatgattc cttggccatt atttgttcac tacgcagcaa gtaaaggcgg tatgaaacta
      721 atgacggaaa cattggctct tgaatatgcg ccaaaaggta tccgagtaaa taacattgga
      781 ccaggtgcga tgaacacacc aattaacgct gaaaaattcg ctgatcctgt acaacgtgca
      841 gacgtagaaa gcatgattcc aatgggttac atcggtaagc cagaagaagt agcagcagtt
      901 gcagcattct tagcatcatc acaagcaagc tatgtaacag gtattacatt atttgctgat
      961 ggtggtatga cgaaataccc ttctttccaa gcaggaagag gctaatagtt tttaatcgat
     1021 gtggaaaaaa gagacatggc aaaagcgcca tgtctctttt ttctatacct aagcgaatgc
     1081 cacccccttg cggggctggt ggcattcgcc taggtaaaag ctt
//