From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Anabaena sp. (clone pAN600) rbcL & rbcS genes encoding both
Date: 15 Sep 92 10:02:09 GMT
Distribution: bionet
Checksum: 31615     8

LOCUS       ANARUBP      3429 bp ds-DNA             BCT       15-SEP-1992
DEFINITION  Anabaena sp. (clone pAN600) rbcL & rbcS genes encoding both
            ribulose-1,5-bisphosphate carboxylase subunits, complete cds.
ACCESSION   J01540
KEYWORDS    carboxylase; ribulose bisphosphate carboxylase.
SOURCE      Anabaena sp. (library: PCC 7120) DNA.
  ORGANISM  Anabaena sp.
            Prokaryotae; Gracilicutes; Oxyphotobacteria; Cyanobacteria;
            Nostocales; Nostocaceae.
REFERENCE   1  (bases 511 to 2363)
  AUTHORS   Curtis,S.E. and Haselkorn,R.
  TITLE     Isolation and sequence of the gene for the large subunit of
            ribulose-1,5-bisphosphate carboxylase from the cyanobacterium
            Anabaena 7120
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 80, 1835-1839 (1983)
  STANDARD  full staff_review
REFERENCE   2  (bases 1 to 720; 2055 to 3429)
  AUTHORS   Nierzwicki-Bauer,S.A., Curtis,S.E. and Haselkorn,R.
  TITLE     Cotranscription of genes encoding the small and large subunits of
            ribulose-1,5-bisphosphate carboxylase in the cyanobacterium
            Anabaena 7120
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 81, 5961-5965 (1984)
  STANDARD  full staff_review
REFERENCE   3  (bases 1 to 720)
  AUTHORS   Nierzwicki-Bauer,S.A., Curtis,S.E. and Haselkorn,R.
  TITLE     Cotranscription of genes encoding the small and large subunits of
            ribulose-1,5-bisphosphate carboxylase in the cyanobacterium
            Anabaena 7120; Correction
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 82, 1568-1568 (1985)
  STANDARD  full staff_review
COMMENT     rbcL [2] was formerly designated rbcA [1].
            The two genes appear to be cotranscribed, beginning with the mRNA
            start given in the FEATURES TABLE and ending with a potential
            termination site (hairpin and poly-T, bases 3061-3092) downstream
            of the rbcS stop codon [1].
            revision    474      505     aagaa...22 bp...aaaag in [3];
                                         ag in [2]
            revision    564      595     ctaaa...22 bp...gtcgc in [1],[3];
                                         cc in [2]
            revision    655      686     gtgag...22 bp...tttga in [1],[3];
                                         ga in [2]
FEATURES             Location/Qualifiers
     mRNA            217..3097
                     /note="contains the rbcL and rbcS genes"
                     /gene="rbc"
     CDS             721..2151
                     /note="ribulose-1,5-bisphosphate carboxylase large subunit
                     (rbcL)"
                     /codon_start=1
                     /translation="MSYAQTKTQTKSGYKAGVQDYRLTYYTPDYTPKDTDILAAFRVT
                     PQPGVPFEEAAAAVAAESSTGTWTTVWTDLLTDLDRYKGRCYDIEPGPGEDNQSIAYI
                     AYPLDLFEEGSITNVLTYIVGNVFVFKALRALRLEDIRFPVAYIKTFQGPPHGIQVER
                     DKLNKYGRPLLGCTIKPKLGLSAKNYGRAVYECLRGGLDFTKDDENINSAPFQRWRDR
                     FLFVADAITKAQAETGEIKGHYLNVTAPTCEEMLKRAEYAKELKQPIIMHDYLTAGFT
                     ANNTLARWCRDNGLLLHIHRAMHAVIDRQKNHGIHFRVLAKALRLSGGDHIHTGTVVG
                     KLEGERGITMGFVDLLRENYVEQDKSRGIYFTQDWASLPGVMAVASGGIHVWHMPALV
                     EIFGDDFVLQFGGGTLGHPWGNARGATANRVALEACVQARNEGRNLAREGNDVIREAA
                     KWSPELAVACELWKEIKFEFEAMDTV"
     CDS             2700..3029
                     /note="ribulose-1,5-bisphosphate carboxylase small subunit
                     (rbcS)"
                     /codon_start=1
                     /translation="MQTLPKERRYETLSYLPPLTDVQIEKQVQYILSQGYIPAVEFNE
                     VSEPTELYWTLWKLPLFGAKTSREVLAEVQSCRSQYPGHYIRVVGFDNIKQCQILSFI
                     VHKPSRY"
BASE COUNT     1046 a    730 c    721 g    932 t
ORIGIN      79 bp upstream of HinfI site.
        1 ggataagtag aggaggcagg ggaagtaaat aagaatgact atggactatt gaccaatgac
       61 tattggcaac tgacaactga ctcctataca gtcattgata cattttgtaa ctgattgtta
      121 acaaaacgtt taaaacttta tgtaataaca aatttaaata tgtaagttaa gaacttttca
      181 aagaataact tatgccattt cttgatatat tgagagacaa gttacaaatt acgtggtgtg
      241 caattttttc atcttgcgct gattactcta ctaaatatcc gtcaagtaaa ttggctctta
      301 gctcgtctcc tgtcaataaa ggaggtcggc aagagtgcag aagcgggaat gtgtgaaaac
      361 taacccaatt cattaaatac cccgaaatat aggggaatca tctcatactt tccgtaaacc
      421 gcgaaggtcg tgaagggata aaagcaattt agtgggtgag aagaacagat aaaaaagaat
      481 tttttaacta tggcaagagg aaaaagtaaa agcgttaact atgcactcct agatgagcaa
      541 gacactggtg aagaggatta ccactaaagc taagtgttag ttgcagaaag gtcgctgacc
      601 tctaccaaaa gattattcct gtttttctcc tgctgatagg gaggtagggc aattgtgaga
      661 ggaaattgta ccaaaacgtg atttgataag taaaaagagt gacatcttgg aaggatagat
      721 atgtcttacg ctcaaacgaa gactcagaca aaatctgggt ataaagccgg ggttcaagat
      781 tacagactaa cttattacac acctgattac acacctaaag atacagatat tctggcggca
      841 ttccgtgtta caccccagcc cggagttccc tttgaggaag cggctgcggc agtagcggct
      901 gagtcttcta ctggtacttg gacgaccgta tggacagacc tgttaaccga tctagatcgt
      961 tacaaaggtc gttgctacga tatcgaacca ggtcccggcg aagacaacca atccattgcc
     1021 tacatcgctt atcctttgga tctgtttgaa gaaggctcca tcaccaacgt tttgacctac
     1081 attgtaggta acgtatttgt ttttaaagca ttacgcgcat tgcgtttgga agacattcgc
     1141 tttcctgttg cttacatcaa gaccttccaa ggccctcctc acggtatcca agttgagcgt
     1201 gacaaattaa acaaatatgg ccgtcctctg ttgggttgta ccatcaaacc aaaattaggt
     1261 ctgtctgcta agaactacgg acgcgctgta tacgagtgtt tgcgcggtgg tttggacttc
     1321 accaaagacg acgaaaacat taactccgca ccattccaaa gatggcgcga tcgcttcttg
     1381 tttgtagctg atgccatcac caaagcacaa gcagaaacag gcgaaatcaa aggtcactac
     1441 ctaaacgtga ccgctcctac ctgtgaagaa atgctaaaac gggctgagta cgctaaagaa
     1501 ctcaaacagc ccatcatcat gcacgactac ctgaccgcag gtttcacagc taacaacacc
     1561 ttggctcgtt ggtgtcgtga caacggtctt ctactgcaca tccaccgcgc gatgcacgca
     1621 gtaatcgacc gtcaaaagaa ccacggtatc cacttccgtg tattggctaa agccctacgt
     1681 ctatctggtg gtgaccacat ccacaccggt accgtagtag gtaaattgga aggtgaacgc
     1741 ggtatcacaa tgggcttcgt tgacctacta cgtgaaaact acgttgagca agacaagtct
     1801 cgcggtattt actttaccca agactgggct tctctacctg gtgtaatggc agttgcttcc
     1861 ggtggtatcc acgtatggca tatgccagcg ttggtagaaa tcttcggtga tgacttcgta
     1921 ctacaattcg gtggtggtac actcggacac ccttggggta acgctcgtgg tgcaaccgct
     1981 aaccgtgtag ctttggaagc ttgcgtccaa gcacgtaacg aaggtcgtaa cttggctcgt
     2041 gaaggtaacg acgttatccg tgaagctgct aagtggtctc ctgaattggc tgtcgcttgc
     2101 gaactgtgga aagaaatcaa gttcgagttt gaggcaatgg ataccgtctg ataaaggctg
     2161 aagatgcaag gatgaaggtt aaatgaaaat acctttcatc cttacattct ttaaacttca
     2221 tccttttaaa gggctgggtc aagcatgaat ctcaagcaaa tagcgaaaga tacagccaaa
     2281 acgctccaaa gctacctgac ttatcaggcg ctaatgactg tgttggcaca gctaggcgaa
     2341 atgaatccgc cgttagcatt atggctgcat actttttccg tcggcaaagt tcaggacgga
     2401 gaagcgtatg ttaaagaact tttccgagaa cagccagatt tagccttgcg gataatgact
     2461 gtcagagaac atatagccga agaagtagcc gagttcttac cagaaatggt tcgtagcggt
     2521 attcagcaag cgaatatgga acaacgccgc cagcatctag aacgcgtgac gcatctgagt
     2581 ttatctaacc ccagtcctga atcagaacag cagacaattt ccgatactga ctgggatcat
     2641 taatccagtt aggccagcat aagtacttat ttccaaatag caaccaatta tcaccagcta
     2701 tgcaaacctt accaaaagag cgtcgttacg aaaccctttc ttacttaccc cccctcaccg
     2761 acgttcaaat cgaaaagcaa gtccagtaca ttctgagcca aggctacatt ccagccgttg
     2821 agttcaacga agtttctgaa cctaccgaac tttattggac actgtggaag ctacctttgt
     2881 ttggtgctaa aacatcccgt gaagtattgg cagaagttca atcttgccgt tctcaatatc
     2941 ctggtcacta catccgtgtt gtaggatttg acaatattaa gcagtgccaa atcctgagct
     3001 tcatcgttca caaacccagc agatactaaa agctgatttg gtttaataat ttatccattc
     3061 aagagaggta gatgacttta cctctctttt ttgtttttac agtgtttggg atatagcatt
     3121 tttcatactc atgaaatcca gtttaattct atggtattaa ataatttaaa caatacaggt
     3181 attcgtttat atgataattc tattaaaaac ttagttttcc aaaaacataa cttactcttt
     3241 ttagtaccat tcataatata tgcaacatgg atattaatta tttctttata tggtgtcaat
     3301 attccgatac tagatcaatg gctagcacct ggtgaacaaa tagagagttt ttttaaaaat
     3361 caattacata ttgaaacact ttataaacaa cataatgaaa gcaggaagtt aattccaaac
     3421 ttgatattt
//