From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Arthrobacter oxidans phenmedipham hydrolase (pcd) gene, complete
Date: 31 Dec 92 01:42:58 GMT
Distribution: bionet
Checksum: 12886 5

LOCUS       ARGPMPH      1948 bp ds-DNA             BCT       18-DEC-1992
DEFINITION  Arthrobacter oxidans phenmedipham hydrolase (pcd) gene, complete
            cds.
ACCESSION   M94965
KEYWORDS    carboxylesterase; phenmedipham hydrolase.
SOURCE      Arthrobacter oxidans (strain P52) DNA.
  ORGANISM  Arthrobacter oxidans
            Prokaryotae; Firmicutes; Irregular asporogenous rods.
REFERENCE   1  (bases 1 to 1948)
  AUTHORS   Pohlenz,H.-D., Boidol,W., Schuettke,I. and Streber,W.R.
  TITLE     Purification and properties of an Arthrobacter oxydans P52
            carbamate hydrolase specific for the herbicide phenmedipham and
            nucleotide sequence of the corresponding gene
  JOURNAL   J. Bacteriol. 174, 6600-6607 (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     RBS             382..386
                     /gene="pcd"
     misc_feature    955..957
                     /gene="pcd"
                     /note="esterase active site (serine)"
     CDS             394..1875
                     /gene="pcd"
                     /evidence="EXPERIMENTAL"
                     /product="phenmedipham hydrolase"
                     /codon_start=1
                     /translation="MITRPIAHTTAGDLGGCLEDGLYVFRGVPYAEPPVGDLRWRAAR
                     PHAGWTGVRDASAYGPSAPQPVEPGGSPILGTHGDPPFDEDCLTLNLWTPNLDGGSRP
                     VLVWIHGGGLLTGSGNLPNYATDTFARDGDLVGISINYRLGPLGFLAGMGDENVWLTD
                     QVEALRWIADNVAAFGGDPNRITLVGQSGGAYSIAALAQHPVARQLFHRAILQSPPFG
                     MQPHTVEESTARTKALARHLGHDDIEALRHEPWERLIQGTIGVLMEHTKFGEWPLAFY
                     PVFDEATIPRHPIESIIDSDIEIIIGWTRDEGTFPFAFDPQVSQADRDQVESWLQKRF
                     GDHAASAYEAHAGDGTSPWTVIANVVGDELFHSAGYRVADERATRRPVRAYQFDVVSP
                     LSDGALGAVHCIEMPFTFANLDRWTGKPFVDGLDPDVVARVTNVLHQAWIAFVRTGDP
                     THDQLPVWPTFRADDPAVLVVGDEGAEVARDLARPDHVSVRTL"
BASE COUNT      360 a    637 c    601 g    350 t
ORIGIN      
        1 gcttcaccag ccgcgaccac taacgttccg gcgcacgtgc gactgcggtg cacgctgaca
       61 cagttatcag ccggtcgttg gacatccttg ccagtcacgg caccccagcc aacccggaag
      121 tggcacctgc tcgggcacat cggtgcgaac gcttcgtcct gattccgatg ccaactgctt
      181 gacggccgtg acacatatgt agcatagtcg cctagcatgg acccgcagca cacctgctgt
      241 cggctcccgc gctatccccg accagcgccg gtcacgggta gtcctcgtga gaggcaccag
      301 aacgacaacg gcgcactgtc ccgcaacacg gccgtataac cccaccgggg tccgcgcccg
      361 agctagttct ggctcaacca taaggagaac ctcgtgatta ccagaccgat cgcccacacc
      421 accgctgggg acctcggcgg ttgccttgaa gacggcctgt acgtgttccg aggagtgccg
      481 tacgccgagc cgccggtcgg cgacctgcgg tggcgggcgg cgcgcccgca cgccggctgg
      541 accggcgtcc gcgacgcctc cgcgtatggt ccctcggcgc cgcaacccgt ggagcctggc
      601 ggctcgccga tccttgggac acacggcgac cctccgtttg acgaggactg cctgactctc
      661 aatctttgga ccccgaacct cgacggcggt agccggccgg tcctcgtctg gatccatggt
      721 gggggcctac taaccggctc gggaaatcta cctaactacg cgaccgatac cttcgcccgc
      781 gacggcgact tggtaggtat ctcaatcaat taccggctcg ggcctcttgg attcctcgca
      841 ggaatgggcg acgagaatgt ctggctcacc gatcaggtag aggcactgcg ctggattgca
      901 gataacgttg ctgccttcgg tggagacccg aaccggatca ctctcgtcgg tcaatcaggc
      961 ggggcatact cgatcgcagc gctcgcccaa cacccggtcg cccgtcagct gttccaccgc
     1021 gcgatcctac aaagcccacc attcgggatg caaccccata cagttgaaga atcgacggca
     1081 aggacgaagg ccctggcccg gcatctcggg cacgatgaca tcgaggccct gcgccatgag
     1141 ccgtgggaga ggctgattca aggcacgata ggcgtcctga tggaacacac caaatttggc
     1201 gaatggcccc tggcattcta tccggtgttc gatgaggcaa cgatacctcg ccatccgatt
     1261 gagtccatta tcgattccga catcgaaatc atcatcggct ggacacgcga cgagggcact
     1321 tttccgtttg ccttcgaccc tcaggtttca caggcggatc gcgatcaggt cgagtcatgg
     1381 ttgcagaagc gtttcggaga ccacgccgcc tcggcctacg aggctcacgc cggcgacgga
     1441 accagtcctt ggaccgttat cgccaacgtt gtgggcgacg agctctttca cagcgctggg
     1501 taccgggtcg cggacgaacg ggcaacgcgc agaccggtac gggcctatca gttcgacgta
     1561 gtctcgccct tgtcggacgg agccctcggc gcggtccact gcatcgaaat gccgttcaca
     1621 tttgccaatc tcgaccgttg gacggggaag ccgttcgtgg acggcctgga tccagacgtg
     1681 gtggctcggg tgaccaacgt gttgcatcag gcctggatcg cattcgtccg aacgggagac
     1741 cccacgcacg accagttgcc ggtgtggcca acgttccgag cggacgaccc agcggtgttg
     1801 gtcgtcggcg acgagggagc agaggtggcg cgggatctag cgcgcccgga ccacgtcagc
     1861 gttcggaccc tatgagggtc gcgggtcgcc ggggtcttga ggccggaggg cctcgcgtat
     1921 gcagtgattc gtggatcacc ggccagtt
//