From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Kas12=beta-ketoacyl-acyl carrier protein synthase I isozyme
Date: 27 Jan 93 05:58:24 GMT
Distribution: bionet
Checksum: 64988 9

LOCUS       S118931      5239 bp    DNA             PLN       20-JAN-1993
DEFINITION  Kas12=beta-ketoacyl-acyl carrier protein synthase I isozyme
            [Hordeum vulgare=barley, cv. Bonus, Genomic, 5239 nt]
ACCESSION   S49422 M95172
KEYWORDS    .
SOURCE      barley cv. Bonus
  ORGANISM  Hordeum vulgare
            Unclassified.
REFERENCE   1  (bases 1 to 5239)
  AUTHORS   Kauppinen,S.
  TITLE     Structure and expression of the Kas12 gene encoding a
            beta-ketoacyl-acyl carrier protein synthase I isozyme from barley.
  JOURNAL   J. Biol. Chem. 267, 23999-24006 (1992)
  STANDARD  full automatic
COMMENT     This entry [NCBI gibbsq 118931] was created by the journal scanning
            component of NCBI/GenBank at the National Library of Medicine.
            This sequence comes from Fig. 4.  Map location: 2.
FEATURES             Location/Qualifiers
     CDS             join(1432..1863,2872..3303,3521..3694,4076..4189,
                     4265..4295,4299..4366,4452..4518,4713..4780)
                     /note="insertion(412-414, missing T).  beta-ketoacyl-ACP
                     synthase I isozyme;  For the protein sequence (NCBI gibbsq
                     118934):  Method: conceptual translation supplied by
                     author.  This sequence comes from Fig. 4.
                     Author-given protein sequence is in conflict with the
                     conceptual translation"
                     /gene="Kas12"
                     /product="beta-ketoacyl-acyl carrier protein synthase I
                     isozyme"
                     /codon_start=1
                     /translation="MHAHAAHALGLRVPPPAFPRRRARPRRRPAAAVLATSAAPQRET
                     DPRKRVVITGMGLASVFGSDVDTFYDRLLAGESGVGPIDRFDASSFPTRFAGQIRGFS
                     SEGYIDGKNDRRLDDCIRYCILSGKKALESAGLGAGSDAHVKLDVGRAGVLVGTGMGG
                     LSVFSDGVQNLIEKGYRKISPFFIPYAITNMGSALLAIDVGFMGPNYSISTACATSNY
                     CFYAAANHIRRGEADIIVAGGTEAAIIPIGLGGFVACRALSQRNDDPITACRPWDKER
                     DGFVMGEGAGVLVMESLEHAMKRDAPIIAEYLGGAVNCDAYHMTDPRADGLGVSSCIT
                     MSLRDAGVAPEEVNYINAHATSTLAGDLAEVRAIKQVFKNPSEIKINSTKSMIGHCLG
                     AAGLEAIATIKSITTGWVHPTTINQFNPEPEVDFDTVANEKKQHEVNVGISNSFGFGG
                     HNSVVVFAPFKP"
BASE COUNT     1312 a   1163 c   1194 g   1570 t
ORIGIN      
        1 tctagacaat atccatgtcc tttcaggtgt tatacgtgat acggggttgc tgcatgtatg
       61 cacgtatatg taccgactct gaccacaaac ataaatatgc aaagtggctt tagcaatccg
      121 attaacactt tgtttatcca acgtagaagg tggagctcgt ctatgaaacc aaagacttaa
      181 aattatacat ctatcctgag gatgatgccc gaggtccttg gcttgtagac ttctccaggg
      241 gctactgaag gtgtcttgag cttgggggta cataccatgt cggcacacca ttcatgggtt
      301 gggccaagca cacgcgcatg ataggaaaat aggtgacata tgcccagccc cgacatgatc
      361 aaggcaaaga cctttgaaga ctttaacact tcaaggccta tttgaatgat tgaaatgctt
      421 atccctagat ggtaaccagc catatgcaaa cccgagatgc cgcccacgtt tgtacaagcc
      481 gtagggttta ggtggtagtg gtagtttttt tggaggctcg gagcttagaa ctccatctta
      541 cgatctcaag atagttcatg ttgtattgtc tactccatca caattaatat accacaaaca
      601 tgatgtaggg ttttacctgc tcaagaggtc ccgaacctga gtaaaacaat gtgttcccat
      661 tttctcatgt tattatgctg cttagatcac cagcttggga ttgcctacag aagatcaacc
      721 ggttagcacc gacaccatag acgacaaggc ctcacccaag aacagagtcg tagccgtcga
      781 cgagctgtcg gaggcaggtt caaagattca tgactcagga tgcggatggg gattctcatt
      841 tgggaatgct agtggttcgt cgtttagagg gatggtcggg ggtggcgcca tcacaacagg
      901 ccaacaatga agggtgggtg aggaggcaag gatagggcgg gacgtcgtgt gtggcggtgg
      961 ctggcggttt gatcgaggat catgggcggt ttggcaagta gatgaacacg gaggacgggg
     1021 ggttgaagat gtcatctcgg cgttcatttt gcatcggacg gttaacaaat cagttgatct
     1081 aatatttttt cagtcaactg atccatgcca ggcccttatt ttcctccatt tgaaatctag
     1141 catgtttctc ttccaaaaag atgtactcct attttttata ccgctcaaaa aaaaacctcc
     1201 tatttttcat aaacacgcag tagtaactga aagcgacagt tctgatttct gcccctccca
     1261 atccaatcca caataaacct ctccctccaa aaatccatcc atccatcttt ccgctgtgtc
     1321 cggcaccgcc accctcgccg cgcggcgcct ctgccacctc ctcctcctcc gccgcaccgc
     1381 ctgtttaacc gcctcactcg cgcccccgcg ggcccgtccg caagctccgc catgcacgcc
     1441 cacgccgccc acgccctcgg cctccgcgtc cctccccccg cgttcccccg ccgccgcgct
     1501 cgcccgcgcc ggcgccccgc cgcggccgtc cttgccacct cggcggcgcc tcagagggag
     1561 acggacccca ggaagcgggt ggtgatcacg gggatggggc tggcgtcggt gttcgggagc
     1621 gacgtggaca cattctacga ccggctcctg gccggggaga gcggcgtcgg gccgatcgac
     1681 cggttcgacg ccagcagttt ccccacgagg ttcgccgggc agatacgggg gttctcgtcg
     1741 gaggggtaca tcgacggcaa gaacgaccgg aggctcgacg actgcatccg gtactgcatc
     1801 ctcagcggca agaaggcgct cgagagtgcc gggctgggcg caggctccga cgcgcacgtc
     1861 aaggtgagtg agatggagag cggggcgtgc ccttgcctcc gagtgaactg agttctctgt
     1921 tttgtgcgga cattgcagaa atagtagact cttgagtatt tggcatcaat tttttcctcc
     1981 ctgagagccc tgcaacatac gagtgtagat tttacttcag gacaagctct gatttatgaa
     2041 caatgtaggt tgtgattgtg ttacatgtgt gtgactctct gactataaat cagtaggaac
     2101 tgtgtatttc tgtatgattt ttcttctgtc tgaatcagtt aggacgtatc catggactga
     2161 aaaaccagtg agcagttttc aaaatacttg agtattgttc ctcaaaaaaa ttgtatactg
     2221 aaatcaaaat gctcaaacaa tgacaatatc ataagttttc aacaggaaaa caggtctaac
     2281 tagatttagt ttgagaaaag atatggattc tttttggaca gttttacacc atatgggttt
     2341 gtgagaaata gctatatata tttgcagctt gatatttgct gaagaaaaca cacgttttat
     2401 cttactgttt ctgagatggc ctcatgtgta ctttttatga atgaaaaaga gacagataaa
     2461 tttgtgtttc ttgatgtaaa atatggttcg cagttttgta tcatgtaaac ctgctttgct
     2521 ttgtgtataa gctcataaat cactacactg cattccaaaa tacctgtgaa cttgtgtgag
     2581 ttcaacttca ataatctccg tctgaggcat gtttgtaaat atcacttgta tgtaatcata
     2641 atcaactgtt ggttgcaaca acagttcata cataaaaata gatgtgaatc gtgaatttat
     2701 ttgatggtcc cttaagcagt tggttgcttc ttctgcccga taggctgtca ataggtgtct
     2761 accatcattt ccatactgcc ataaattcta tttgttctct aggtttctat tggttcattt
     2821 ttttctcgtc ttttcaaatg tctcttttgc taatgaatta attattaaca gcttgatgtg
     2881 gggcgggctg gtgttcttgt ggggactggc atgggtggcc tttcagtatt ctccgatggt
     2941 gttcagaacc ttattgagaa gggatacaga aaaatttcac ctttctttat cccatatgct
     3001 ataactaata tgggttcagc cttgcttgcc atagatgttg gtttcatggg ccccaactac
     3061 tcaatttcaa ctgcgtgtgc aacctccaac tactgctttt atgctgccgc aaaccatatt
     3121 cgtcgtggtg aagctgatat catcgtcgct ggtggaactg aagctgccat cattccaatt
     3181 ggccttggag gttttgtagc gtgtagagca ctatcacaaa ggaatgatga cccaataact
     3241 gcatgtaggc catgggataa agagcgagat ggctttgtta tgggtgaagg tgccggtgta
     3301 ctggtatggt cgattaacaa aaaatatatc atgtttttaa atacttttat gtactttaga
     3361 atcatgttag ccatctacat tccttctttg gtgtttgttg tttgcttcag gttgttatat
     3421 ttattctcag tttgacacca tgaacttcag tcatcagtta gatcctattg ctattagcga
     3481 catgtaatgc ttagacggtg aaactcttgt tcaataatag gttatggaga gcctggagca
     3541 cgcaatgaag cgcgatgcac cgataatagc tgaatacttg ggaggtgcgg tcaactgtga
     3601 tgcttaccat atgactgatc ctagagcaga tggactgggt gtatcatctt gtatcacaat
     3661 gagtctcaga gatgcaggtg ttgcaccaga ggaggtatat cttgtatcaa cttcccaacc
     3721 attttttgta catctgcagt tatttttcct tcctagcatg tcatctaata tccgaacttc
     3781 cgaagttaaa atattggcgt gaccgatatt tatgttcaaa ttttcatctg gggggcctgt
     3841 cggcaagatt cttttctgtt aagtgaaata cacccccccc ccaaaaaaaa aaagaactgg
     3901 gcgggtttga aaacacggta ctgtcacatt attagaatat ttattaattt gtttataagc
     3961 ttagtgttcc cctgcttagc tgcagttttg gttcatactt ttttctcaat tcttgtttga
     4021 ttcgtaatgc agagtatcag tcagcagatt ttgattccat ttgatgtatt tttaggtgaa
     4081 ctacatcaat gcacacgcaa cttcgacact tgctggtgac ttggctgaag taagagccat
     4141 caaacaagtc ttcaagaatc catctgagat taagatcaat tctacaaagg ttcgctcgct
     4201 tgcttccatg tcatttccta gttgttctcc tatctgatta tctattgtca cttccttgcg
     4261 gcagtccatg ataggccatt gcctgggtgc ggcgggtggg ctagaagcta ttgccactat
     4321 caagtccata actacaggat gggtgcatcc taccattaac caatttgtaa gctagtcatt
     4381 accatgtctg aatctcgttg tcacacatac cgtcagagtt tctcatgttt tgagttattt
     4441 ctcttctgca gaacccggag cctgaagttg attttgatac agtagctaac gaaaagaagc
     4501 agcatgaagt gaatgttggt gagttcctcc atccctgaac agcgcctccc ccagccccat
     4561 cccccttctc tcttaaaagt ggtacataat gaacggtagc caaatgctca gcagaatagc
     4621 tcacatagaa tctaccatcc attcacaagt gcatacaatt atcaccccca gcatctctat
     4681 ctttacgcgt gtttggtatc ttgtaaatgc aggtatttct aactcattcg gatttggagg
     4741 gcacaattcc gtggtagtat tcgcgccatt caagccgtga tacgtctttg cttcagggat
     4801 gtatgatccg cgacatcctc ttgattagat gttcttaggc tgcttgaatt taaaagattt
     4861 tatttctcct ggtggagcaa gttttgatga ttggtagagt ggtgctgcct tgacctggac
     4921 actgttgttc atatgataat tctttcgggt gcatcccgcg tgtaccaagt tgtagaaaat
     4981 ctggaatgat gtaaaggatc cttcagtatt acgtatgaat ttggtactcc atagttcctt
     5041 tggatgttct tcacccttcc cttcgctgca caaagtgttg ctgtagacag ttttccgact
     5101 aaatgtctag tagtaatttt ttttttggcg aatagtagta aatacagtat ctttgaagca
     5161 gtaatattgt tgatgttatc atggaatcag ttttgttttt actggttaag gaaagtgata
     5221 ataataagat taatgccac
//