From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Yarrowia lipolytica pyruvate kinase gene, complete cds.
Date: 28 Aug 92 10:01:24 GMT
Distribution: bionet
Checksum: 35799     6

LOCUS       YSJPYKI      2794 bp ds-DNA             PLN       28-AUG-1992
DEFINITION  Yarrowia lipolytica pyruvate kinase gene, complete cds.
ACCESSION   M86863
KEYWORDS    pyruvate kinase.
SOURCE      Yarrowia lipolytica (library: NRRL Y-1094) DNA.
  ORGANISM  Yarrowia lipolytica
            Eukaryota; Fungi; Eumycota; Hemiascomycetes; Endomycetales;
            Saccharomycetaceae.
REFERENCE   1  (bases 1 to 2794)
  AUTHORS   Strick,C.A., James,L.C., O'Donnell,M.M., Gollaher,M.G. and
            Franke,A.E.
  TITLE     The isolation and characterization of the pyruvate kinase-encoding
            gene from the yeast Yarrowia lipolytica
  JOURNAL   Gene 118, 65-72 (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     CDS             join(418..532,1126..2594)
                     /product="pyruvate kinase"
                     /gene="pyruvate kinase"
                     /codon_start=1
                     /translation="MIYTANSSPSTNLQGPSTLNTDDIPTKNYRKSSIIGTIAGLNIV
                     RMNFSHGSYEYHQSVIENARESEQRFRGRPLAIALDTKGPEIRTGVTKDDKDWDVKAG
                     HVMLFSTNPKYKDQCDDKIMYIDYTNIVKQIDIGKIIFVDDGVLSFKVLEKIDGETLK
                     VETLNNGKISSRKGVNLPGTDVDLPALSEKDKADLKFGVEHGVDMIFASFVRTANDVQ
                     AIRDVLGEKGKGIQVISKIENQQGVNNFDEILKETDGVMVARGDLGIEIPAPQVFIAQ
                     KQLIAKCNLAGKPVICATQMLDSMTYNPRPTRAEVSDVGNAVLDGADCVMLSGETAKG
                     TYPIESVKMMHETCLVAEKAIAYAPLFNEMRTLTVRPTETVETIAISAVSASFEQQAR
                     AIIVLSTSGTSARLCSKYRPNCPILMVTRNAQAARFSHLYRGVYPFIYHKARASNPAE
                     WQHDVEERLKWGMDEAVALGILNKGDVVVAIQGWTGGLATPTLSEFSSVSKLFNTNLL
                     DYKTYSMVIKSVNGGVLYK"
     5'UTR           375..417
                     /gene="pyruvate kinase"
     exon            375..532
                     /number=1
                     /gene="pyruvate kinase"
     intron          533..1125
                     /number=1
                     /gene="pyruvate kinase"
     exon            1126..2594
                     /number=2
                     /gene="pyruvate kinase"
                     /partial
BASE COUNT      674 a    787 c    684 g    649 t
ORIGIN
        1 gtaacatgac gttgaccgtg cggggctaca tgtagcagct gggtgtgcta actacggata
       61 catgcctaca acccccacaa gtcaagacca ttgcgacgcg gaaacaggag cccgcaaaag
      121 aggagaaaaa caacggcgag actcgggggc ggagtgggtc acgtgacttt cctttttccc
      181 ctcacctggc ccgctccgtc catatctctg tcgtacaaga caatattgtc gcaacgcaaa
      241 aggtccataa attactgggt agacgcaact ctatttgaag gcaacctacc gtttgctttt
      301 agtgttttgg ttttgttacc atatccaaaa aaaaaccata tatccaaaaa ttccgctgca
      361 ccatctcttc ttctctccat caactacccc tgcggagaaa ttcacaccac agttacaatg
      421 atttacaccg ccaattcgtc cccttccacc aacctgcagg gtcctagtac cctgaacacg
      481 gatgacattc ccaccaagaa ctaccgaaag tcgtccatca ttggtactat cggtgagtat
      541 acttatccac agaccagacg ccgattgcgc ggtttggtgc acaattcgac gagcccacaa
      601 gaggtaggcg tcacaggata acggacccgc tcatgtgaac atgtggcgag cccattgtac
      661 ccgtgtcgcc tgcccccaag tcgattcgcc gaatgcgctc aaacgctggc tcggtctcgc
      721 ctcaggcctc agtaaaaacg gcaaactaac agcaggtccc aacacaaact ccgcccgggg
      781 tggtctccaa gcttcgacaa ggtgagtaac cataatgcga cagacgtgtg cgccgtgacc
      841 cgattcgcgg tggccacgtc tatctgtccc ttctttcgtt accccaattg gcaccgtcgc
      901 cttatttttt ggctttggtt tcccgggttt gtccaataca cggctcatgc gcatgcacat
      961 tttttccggt cggataaacc caacgaactc taagtgacaa acatgaaatg aaaacgacgc
     1021 aagtggtaag ggcgctaatg gtgacgttca tgacgttgcc agtctggtgc ccttatcgat
     1081 gacgtatgga cccatgtgtc tatcatgccg caatactaac cacagctggt ctcaacattg
     1141 ttcgaatgaa cttctcccac ggctcgtacg agtaccacca gtccgtcatt gagaacgccc
     1201 gagagtccga gcagcggttc cgaggccgac ccctagccat tgctctcgac accaagggcc
     1261 ccgagatccg aaccggtgtc accaaggacg acaaggactg ggacgtcaag gccggccatg
     1321 tcatgctctt ctccaccaac cccaagtaca aggaccagtg cgacgataag atcatgtaca
     1381 ttgactacac caacattgtc aagcagattg acattggcaa gatcatcttc gttgacgacg
     1441 gtgttctgtc cttcaaggtt ctcgagaaga ttgacggcga gaccctcaag gtcgagactc
     1501 tcaacaacgg taagatctct tctcgaaagg gtgtcaacct ccctggcacc gatgtcgatc
     1561 tccccgctct gtccgagaag gacaaggccg atctcaagtt tggtgttgag cacggtgtcg
     1621 acatgatttt tgcctctttc gtccgaaccg ccaacgacgt ccaggccatc cgagacgtgc
     1681 tcggcgagaa gggcaagggc atccaggtca tctccaagat tgagaaccag cagggtgtca
     1741 acaactttga cgagattctg aaggagaccg acggtgtcat ggttgcccga ggagacttgg
     1801 gtatcgagat ccccgccccc caggttttca ttgcccagaa gcagctgatc gccaagtgta
     1861 acctggccgg aaagcccgtc atctgtgcca cccagatgct cgattccatg acctacaacc
     1921 cccgacccac ccgagccgag gtctctgatg tcggtaacgc cgtccttgat ggtgctgact
     1981 gtgtcatgct gtctggtgag acggccaagg gaacctaccc cattgagtcc gtcaagatga
     2041 tgcacgagac ctgtctggtt gccgagaagg ccattgccta tgctcctctg ttcaatgaga
     2101 tgcgaaccct gactgtccga cccaccgaga ccgtcgagac cattgctatc tccgccgtgt
     2161 ctgcttcttt cgagcagcag gcccgagcta ttattgtgct gtccacatcg ggaacctccg
     2221 cccgactgtg ttccaagtat cgacccaact gtcccattct tatggtgact cgaaacgcac
     2281 aggccgctcg attctcccat ctgtaccgag gcgtctaccc cttcatctac cacaaggccc
     2341 gagcttccaa ccctgccgag tggcagcacg atgttgagga gcgactcaag tggggtatgg
     2401 acgaggctgt tgctcttggt attcttaaca agggcgatgt tgttgtcgcc atccagggat
     2461 ggaccggtgg tctggccaca ccaacactct ccgagttctc gagtgtgtct aaacttttta
     2521 acacaaacct attagattat aaaacataca gcatggtgat taagtcggtg aacggagggg
     2581 ttttgtacaa gtagtgagac ggcacagtat gtagtagtag ccggacttaa gaagtggcct
     2641 atcaagaaga gctggagtgg atttataatt ggcatctagt ttagattgta gatcctggca
     2701 tgtgcactca gcgggggatg catttgtaat gtggctgctt gattcttcgt gtgtgttaca
     2761 cactcgtaca tattctgtac aatactgtaa gctt
//