From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Ruminococccus flavefaciens beta-glucanse (celD) gene, complete cds.
Date: 6 Nov 92 18:34:54 GMT
Distribution: bionet
Checksum: 45574 4

LOCUS       RUMCELDX     1695 bp ds-DNA             BCT       02-NOV-1992
DEFINITION  Ruminococccus flavefaciens beta-glucanse (celD) gene, complete cds.
ACCESSION   L05368
KEYWORDS    beta-glucanase; celD gene; cellulase.
SOURCE      Ruminococcus flavefaciens (strain FD-1) DNA.
  ORGANISM  Ruminococcus flavefaciens
            Prokaryotae; Firmicutes; Firmibacteria; Gram-positive cocci;
            Streptococcaceae.
REFERENCE   1  (bases 1 to 1695)
  AUTHORS   Howard,G.T. and White,B.A.
  TITLE     Nucleotide sequence of the celD beta-glucanase gene from
            Ruminococcus flavefaciens FD-1
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     -35_signal      171..176
                     /gene="celD"
     -10_signal      193..198
                     /gene="celD"
     RBS             332..337
                     /gene="celD"
     CDS             358..1446
                     /gene="celD"
                     /product="cellulase"
                     /codon_start=1
                     /translation="MQLRLMQTEISQRSWCRRAMDRPITTTGASPEDQGERTDQLFWR
                     DTGGDIAREYAQRYRAEYAATLAQYKVNFRKIQKIHGSPISGAAGRAERRSGPATLSA
                     SIQSINCCREARVSSSPVNSLQTVAIATGIVVSRSSFGMASFSSVPNDQGELHDPPCC
                     AKKRLAPSVLRSLSECKLAAVLRSTSTILKSSPRKICAWQAATAYKAPKRISIKMNWQ
                     VTRYLILMTITESVNWTESILHSIMNGEINNNWNTASDWFNNHCKSDNYFFEGECGSA
                     RYNCAAHLAALVASNHDAVDKEAWAPNQMGYILGGENQMGYILGEKGVGTANPKCFVT
                     GFDENSPKNPHHSAASGTDSAQTNADRH"
BASE COUNT      463 a    392 c    427 g    413 t
ORIGIN      
        1 aaaagtttag ctggtagcta tcatattact agctataact tggtatattt caaatgtatg
       61 ttcttttgca tatgttccag ttactgtaat actggatgtg tcttctgcac taacggtcat
      121 aatactcatc ggcattgctg cacaagcagt aagtgttaaa gcggctgcca ttgcgataaa
      181 tctcttagta gttttcataa tcatctacct tttcatgaag attcacggcg gggagggagt
      241 cgatcagctc ttgtcgagag atcttattct tctttgccga attcaaagat agctacactg
      301 atcttggtca gagagcatta cagatcatcg caagagattt gcagactttt ttctacaatg
      361 cagttaagac tgatgcaaac ggaaatatca cagagatcct ggtgcagaag ggcgatggac
      421 agaccgatca cgactactgg ggcgtcaccg gaagatcagg gcgagcgaac cgatcagctc
      481 ttctggagag ataccggcgg cgatatcgcg cgagaatatg cacagcgata tcgcgcagaa
      541 tatgcagcga cactcgcaca gtataaggtt aatttccgga agatccagaa gatccatggc
      601 tcaccaatat ctggagcagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
      661 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
      721 agtttgcaga cggttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
      781 gcttcattca gctccgttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
      841 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aatgtaagtt ggcggcagtg
      901 ttacgcagca ctagtacgat tctcaaatcg agtccaagga agatatgtgc atggcaggca
      961 gcgactgctt acaaggcacc caagaggatc agtataaaaa tgaactggca agttacccgg
     1021 tacctaatcc taatgacgat aactgagtca gttaactgga cagagtcgat cttgcactct
     1081 atcatgaacg gtgagatcaa caacaactgg aatactgctt ctgattggtt taataaccac
     1141 tgcaaatcgg ataactattt cttcgaaggc gaatgcggct ccgctcgcta taactgcgcc
     1201 gcccacctcg ctgcgctggt tgcttccaac catgatgcag ttgacaaaga ggcatgggcg
     1261 ccaaaccaga tgggatacat cctcggcggc gaaaaccaga tgggatacat cctcggcgaa
     1321 aagggtgtag gtacagccaa tccgaagtgc tttgttaccg gattcgatga gaactcacca
     1381 aagaatccgc atcacagcgc tgcatccgga accgattccg cgcagacaaa tgccgacagg
     1441 cattgagaat aaacacattc tgctgggtgc attggttggc ggacctgcag atgaaaacgg
     1501 cgcatatcaa gatatcagat ctgattatca gagcaatgag gtatcgctgg actacaatgc
     1561 cggacttgtc ggagcggcgg atggcttgta ttatttctac ccccagggta tcactgatgc
     1621 ggatatctca gcaagtgtac ggattccggc gtcgaaataa catacggcgg cggcatggga
     1681 aatagtgcag ccagg
//