From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: C.longicaudatus HPRT mRNA for hypoxanthine guanine
Date: 24 Jul 92 10:05:50 GMT
Distribution: bionet
Checksum: 56451     4

LOCUS       CRUHPRTM     1222 bp ss-RNA             ROD       24-JUL-1992
DEFINITION  C.longicaudatus HPRT mRNA for hypoxanthine guanine
            phosphoribosyltransferase
ACCESSION   X17656
KEYWORDS    hprt gene; hypoxanthine-guanine phosphoribosyltransferase;
            point mutation.
SOURCE      Cricetulus longicaudatus RNA.
  ORGANISM  Cricetulus longicaudatus
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Rodentia; Myomorpha; Cricetidae; Cricetinae; Cricetini.
REFERENCE   1  (bases 1 to 1222)
  AUTHORS   Rossiter,F., Muzny,M., Caskey,T. and Fox,M.
  TITLE     A Chinese hamster HPRT point mutation reverts to the wild-type
            sequence
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
COMMENT     *source: tissue=lung; *source: cell_type=fibroblast; *source:
            cell_line=TG15 derived from V79S; *source: clone_library=lambda
            gt10; *source: chromosome=short arm X;
            See also J00060
            From EMBL    entry CLHPRTM;  dated 21-MAY-1992.
FEATURES             Location/Qualifiers
     CDS             17..673
                     /EC_number="2.4.2.8"
                     /product="hypoxanthine guaninephosphoribosyltransferase"
                     /gene="HPRT"
                     /codon_start=1
                     /translation="MATRSPSVVISDDEPGYDLDLFCIPNHYVEDLEKVFIPHGVIMD
                     RTERLARDVMKEMGGHHIVALCVLKGGYKFFADLLDYIKALNRNSDRSIPMTVDFIRL
                     KSYCNDQSTGDIKVIGGDDLSTLTGKNVLIVEGIIDTGKTMQTLLSLVKRYNPKMVKV
                     ASLLVKRTSRSVGYRPDFVGFEIPDKFVVGYALDYNEYFRDLNHICVISETGKAKYKA
                     "
     mutation        420
                     /note="TG15 point mutation"
     mutation        480
                     /note="RJK159 point mutation"
     polyA_signal    1118..1123
     polyA_signal    1204..1209
BASE COUNT      362 a    206 c    257 g    397 t
ORIGIN
        1 gccgaccgat tccgtcatgg cgacccgcag ccccagcgtc gtgattagtg atgatgaacc
       61 aggctatgac ctagatttat tttgtattcc taatcactat gtcgaggatt tggaaaaggt
      121 gtttattcct catggagtga ttatggacag gactgaaaga cttgcccgag atgtcatgaa
      181 agagatggga ggccatcaca ttgtggccct ctgtgtgctg aaggggggct ataaattctt
      241 tgctgacctg ctggattaca ttaaagcact gaatagaaat agtgatagat ccattcccat
      301 gactgtagat tttatcagac tgaagagcta ctgtaatgat cagtcaacag gggacataaa
      361 agttattggt ggggatgatc tctcaacttt aactggaaag aatgtcttga ttgttgaggg
      421 cataattgac actggtaaaa caatgcaaac tctgctttcc ctggtcaagc ggtacaaccc
      481 caaaatggtt aaggttgcaa gcttgctggt gaaaaggacc tctcgaagtg ttggatatag
      541 gccagacttt gttggatttg aaattccaga caagtttgtt gttggatatg cccttgacta
      601 taatgagtac ttcagggatt tgaatcatat ttgtgtcatt agtgaaactg ggaaagccaa
      661 atacaaagcc taagatgaga gttcaagttg aatctgcaaa cacgaggagt cccattcatg
      721 ttcccagtaa aattaccaag cattctagtt ctgcagccat ctgcttagta cagctttttg
      781 catgaacctt ctaagaattt tatggttttt tatttttaga aatgtcagtt gctgcattct
      841 taaacttttt atttgcacta tgagccttcg atagattgtc gcttaccttg tgagcaaagt
      901 aaatctctta aattaccact attaaaaatg ctgagattgt atctgtaaga aacatttaaa
      961 gaggagatat attagttttt taattggtat tttaattttt atatattcgg gaaagaaagc
     1021 tgtgattgaa tattgttaat tataccacca tgtgtttaga acagtgtgaa gcactcagtt
     1081 tcatgtcagt aaccatctaa gaagttttgc tcagtggaat aaacatatgt tttatcagtg
     1141 ttgcctatat tttcccactt ggattctttc agtgagtcat tgtcaacagt tcctttaaat
     1201 gcaaataaat tctaaaaatt ta
//