From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: E.coli pepP, ubiH (visB) and visC genes.
Date: 13 Jan 93 05:46:35 GMT
Distribution: bionet
Checksum: 13724 11

LOCUS       ECOPUV       4948 bp ds-DNA             BCT       13-JAN-1993
DEFINITION  E.coli pepP, ubiH (visB) and visC genes.
ACCESSION   D90281
KEYWORDS    pepP; proline amino peptidaseII; ubiH (visB);
            ubiquinone biosynthesis; visC; visible light sensitivity.
SOURCE      E.coli (strain K12 CA274) DNA.
  ORGANISM  Escherichia coli
            Prokaryotae; Bacteria; Gracilicutes; Scotobacteria; Facultatively
            anaerobic rods; Enterobacteriaceae.
REFERENCE   1  (bases 719 to 2569)
  AUTHORS   Yoshimoto,T., Tone,H., Honda,T., Osatomi,K., Kobayashi,R. and
            Tsuru,D.
  TITLE     Sequencing and High Expression of Aminopeptidase P Gene from
            Escherichia coli HB101
  JOURNAL   J. Biochem. 105, 412-416 (1989)
  STANDARD  full automatic
REFERENCE   2  (bases 1 to 4948)
  AUTHORS   Nakahigashi,K., Miyamoto,K., Nishimura,K. and Inokuchi,H.
  TITLE     Isolation and characterization of a light-sensitive mutant of
            escherichia coli K-12 with a mutation in a gene that is required
            for the biosynthesis of ubiquinone
  JOURNAL   J. Bacteriol. 174, 7352-7359 (1992)
  STANDARD  full automatic
COMMENT     Submitted (16-JAN-1991) to DDBJ by: Kenji Nakahigashi Faculty of
            Sciences, Kyoto Univ. Sakyo-ku, Kyoto 606 Japan Phone:
            075-753-4200 Fax:    075-791-0271.
FEATURES             Location/Qualifiers
     -35_signal      409..414
     -10_signal      432..437
     misc_feature    444
                     /note="transcription initiation site"
     CDS             477..1061
                     /note="ORF194"
                     /codon_start=1
                     /translation="MLMSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGND
                     DSSWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFD
                     RADALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSL
                     EEIIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     CDS             1087..2412
                     /note="proline amino peptidaseII"
                     /codon_start=1
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADVIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENECEMRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     DVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     AEVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMVAARKQ"
     CDS             2409..3587
                     /note="UbiH;  (VisB) protein"
                     /codon_start=1
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARTQSHVEVTLESGETLTGRVLVA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGKITHAGKRSAYPLALTHAARS
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAQERGEDMGDYGVLCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVVAQRTLGWVAR
                     "
     CDS             3610..4812
                     /note="VisC protein"
                     /codon_start=1
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSALRVAVLEQRVQEPLAANA
                     PPQLRVSAINAASEKLLTRLGVWQDILSRRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAHQSSDITLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFASHRLALVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGTNPAKKLLRDIGLKLADTLPGVKAQLIRQAMGL
                     NDLPEWLR"
BASE COUNT     1111 a   1265 c   1403 g   1169 t
ORIGIN      63 min on K12 map.
        1 gttagttttt tcggtgatgc gaccttgttc aagtaacgct tgttctatgg tctgctgcag
       61 catccgaata cgctgttcca tacttgccgc gtagtcacga gtctttgctt tttcttgcgc
      121 taactcatag ctgatattca atgcggcaat gaagaccaac tgttcagtat ttgtgactct
      181 agtgcgttct ttcagatctt gcaaccgttg gttcagatcg tccgctgcct gattcaacgc
      241 atccctttgg tcaggcgggc agttcacacg caggtaacgg ccaaaaattt ggatatcgac
      301 gggttgtgca gacatgccac cttcctgctg attgactgcg ctgccttcgt ctttagaccc
      361 atggtctgcg aaggggcgac actatagcta ccctgatgag aagagacaag cccttttctg
      421 gtccaccagg gcccaaagtg gtagcatatc atgaatattc ctccctttga cgacgaatgc
      481 ttatgtctat acagaacgaa atgcctggtt acaacgaaat gaaccagtat ctgaaccaac
      541 aagggacggg tctgacccca gctgagatgc atggtttaat cagcgggatg atatgtggcg
      601 gtaacgatga cagctcatgg ctaccgctac ttcacgacct gacgaacgaa ggcatggctt
      661 tcggtcatga gctggcacag gcactgcgta aaatgcactc tgccaccagc gatgccctgc
      721 aggatgacgg cttccttttt cagctttatc tgcctgatgg cgatgatgtc agcgttttcg
      781 atcgggctga tgcattggca ggttgggtca atcacttcct gcttggtctt ggcgttacgc
      841 aaccgaagct ggataaagtg accggcgaaa ccggtgaagc tatcgacgat ctgcgtaaca
      901 ttgcgcaact gggttacgac gaagacgaag atcaggaaga gcttgaaatg tcgcttgaag
      961 agatcatcga atacgttcgt gttgccgcgc tgttatgcca cgacaccttt actcatccgc
     1021 aaccgaccgc gccagaagta caaaaaccga ctctacacta aaaacaaaaa acgtaaggag
     1081 agtgttatga gtgagatatc ccggcaagag tttcagcgtc gccgtcaggc cctggtggag
     1141 caaatgcaac ccggcagcgc cgcgctgatt tttgctgcac cagaagtaac acgtagcgcc
     1201 gacagcgaat acccctatcg tcagaacagt gacttctggt acttcaccgg ctttaacgaa
     1261 ccggaagcgg tgctggtgct gattaaaagc gatgacactc ataaccacag cgttctgttt
     1321 aaccgcgttc gcgacctgac ggcggagatc tggtttggcc gtcgcttagg ccaggatgcc
     1381 gcgccagaga aactgggcgt tgaccgcgca ctggcattca gcgaaatcaa tcagcaactt
     1441 tatcaactac ttaacggcct ggatgtggtt taccatgccc agggcgaata tgcatatgct
     1501 gatgtaatcg tgaacagtgc gctggaaaaa ctgcgtaaag gttcgcggca aaatctcacc
     1561 gcaccggcaa cgatgatcga ctggcgtcct gttgttcatg aaatgcgcct gttcaaatcg
     1621 ccagaagaga ttgccgtact ccgccgcgcg ggagaaatca ccgccatggc acatacacgg
     1681 gcgatggaaa aatgccgtcc gggaatgttc gagtaccatc tggaaggcga aattcaccac
     1741 gaatttaacc gccacggtgc gcgctatccg tcctataaca ccattgtcgg cagcggtgaa
     1801 aacggctgca ttctgcacta caccgaaaac gagtgtgaaa tgcgcgacgg cgacctggtg
     1861 ttgattgacg cgggttgtga atacaaaggt tacgctggcg atattacccg caccttcccg
     1921 gtcaacggca aattcaccca ggcccagcgt gaaatctacg acattgtgct ggagtctctc
     1981 gaaaccagcc tgcgcctgta tcgtccggga acttccattc tggaagtcac tggtgaagtg
     2041 gtgcgcatca tggttagcgg cctggtaaaa ctcggcatcc tgaaaggtga tgttgatgaa
     2101 ctgatcgctc agaacgccca tcgtcctttc tttatgcatg gccttagcca ctggttagga
     2161 ctggatgtcc atgacgtggg tgtttatggt caggatcgct cgcgcattct ggaaccgggc
     2221 atggtactga ccgtagagcc agggctgtat attgcgccgg atgcagaagt gccagaacaa
     2281 tatcgcggta tcggcattcg tattgaagac gacattgtga ttaccgaaac cggtaacgaa
     2341 aacctcaccg ccagcgtggt gaaaaagccg gaagaaatcg aagcgttgat ggttgctgcg
     2401 agaaagcaat gagcgtaatc atcgtcggtg gcggcatggc gggcgcgacg ctggcgctgg
     2461 ctatttcccg gttaagtcac ggggcgctgc cggtacattt gattgaagcg actgcgccag
     2521 agtcacatgc tcatccgggc tttgatggac gagcgatagc gctggcggcg ggtacctgtc
     2581 agcaactggc gcgcatcggc gtctggcaat ctctggcgga ttgcgcaact gccatcacca
     2641 ccgtgcatgt cagcgatcgt ggtcacgctg gatttgtcac cctcgccgca gaagattacc
     2701 aactggcggc gctgggacag gttgtcgaat tgcacaatgt cgggcaacgg ctgtttgcat
     2761 tgctgcgtaa agcacctggc gtaacgctgc attgccctga tcgcgtggct aacgttgccc
     2821 gtactcagag tcacgttgaa gtgacgctgg agagtggcga gacgctgacg ggccgcgtgc
     2881 tggtagcagc tgatggcacc cattcagcgt tagccaccgc gtgcggcgtt gactggcagc
     2941 aggagcctta cgaacaactg gccgtgattg ccaacgttgc tacttccgtt gcgcatgaag
     3001 ggcgcgcttt tgaacgcttt acgcaacatg gcccgctggc gatgttgccg atgtctgacg
     3061 gacgctgttc gctggtctgg tgtcatccac tggaacggcg cgaagaggtg ttgtcgtgga
     3121 gtgacgagaa gttttgccgt gaactccagt cggcctttgg ctggcgactt gggaaaatta
     3181 cccacgctgg taaacgcagt gcttatccgc tggcgttaac ccacgccgcc agatctatta
     3241 cccatcgtac cgtgctggtg ggcaatgcgg cgcaaactct gcacccgatt gccgggcaag
     3301 ggtttaacct cggtatgcga gatgtgatga gtcttgcgga aaccctgact caggcgcagg
     3361 agcgcggaga agacatgggg gattacggcg tattgtgccg ttatcagcag cgtcgacaga
     3421 gcgatcgcga agcaaccatt ggcgtcacgg acagccttgt acatcttttt gccaaccgtt
     3481 gggcaccgct ggttgtcggg cgcaacatcg ggctgatgac gatggaatta ttcaccccgg
     3541 cacgcgatgt cgtggcgcag cgcaccctcg gttgggtggc gcgttgaagg cggttttaag
     3601 gaataaacaa tgcaaagtgt tgatgtagcc attgttggcg gcggcatggt ggggctggcg
     3661 gttgcctgtg gcttacaggg gagcgcgtta cgcgttgccg tactggagca gcgcgtacag
     3721 gaacctctgg cggcgaatgc accaccacaa ctgcgcgttt cggctatcaa tgccgccagc
     3781 gaaaaattac tcacccgtct tggcgtctgg caggacattc tctctcgtag ggccagctgt
     3841 tatcacggta tggaagtgtg ggacaaagac agctttggtc acatttcgtt tgacgatcaa
     3901 agcatgggct atagccatct tgggcatatc gttgaaaatt cagtgattca ctacgcgctg
     3961 tggaacaaag cgcatcagtc gtcagatatc actctgttag cccccgcaga attacagcag
     4021 gtcgcctggg gagaaaatga aaccttcctg acgctgaaag atggcagcat gttaacggcg
     4081 cgtctggtga ttggcgcgga cggcgctaat tcctggttgc gcaacaaagc cgatattccg
     4141 ctgactttct gggattatca gcatcacgcg ctggtagcga ccattcgcac ggaagaaccg
     4201 catgatgcgg tggcgcggca ggttttccat ggcgaaggca ttctggcctt tttaccgctt
     4261 agcgatccgc atctttgctc gattgtctgg tcactgtcgc cagaggaagc gcagcggatg
     4321 cagcaggcaa gtgaagacga atttaatcgc gcgttaaata tcgcttttga taatcgcctg
     4381 ggcttatgca aggttgagag cgcgcgtcag gtgttcccac tgacggggcg ttatgcgcgc
     4441 cagtttgcct cgcaccgtct ggcgctggtg ggcgacgccg cacataccat tcacccgctg
     4501 gcggggcagg gggtaaatct cggctttatg gatgctgcag agctgattgc cgaactgaaa
     4561 cggttgcatc gtcaggggaa agacatcggg cagtacattt atctgcgtcg ctatgagcgt
     4621 agccgcaagc acagtgcggc gttgatgctg gctggtatgc agggattccg cgatctgttt
     4681 tccggtacca atccggcgaa aaaactgctg cgtgatattg gtttgaaact ggccgacacg
     4741 cttcctggcg ttaaggcgca acttatccgc caggcaatgg gattaaacga tttgcctgaa
     4801 tggctgcgtt aaaaatttct cctctgttgt ttatttgata cccatcacac tttcatctcc
     4861 cggttttttc gccgggagat tttcctcatt tgaaataaac taatttcacc tccgttttcg
     4921 cattatattt tctaatgcca ttattttt
//