From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Feline immunodeficiency virus complete genome.
Date: 29 Jul 92 10:01:48 GMT
Distribution: bionet
Checksum: 65018    26

LOCUS       FIVCG        9474 bp ss-RNA             VRL       29-JUL-1992
DEFINITION  Feline immunodeficiency virus complete genome.
ACCESSION   M25381 M25729
KEYWORDS    complete genome; env gene; gag protein; long terminal repeat;
            pol protein.
SOURCE      Feline immunodeficiency virus, Petaluma strain, clones FIV-14 [1],
            [3], and 34TF10 [2].
  ORGANISM  Feline immunodeficiency virus
            Viridae; ss-RNA enveloped viruses; Positive strand RNA virus;
            Retroviridae; Lentivirinae.
REFERENCE   1  (bases 1 to 355)
  AUTHORS   Olmstead,R.A., Barnes,A.K., Yamamoto,J.K., Hirsch,V.M.,
            Purcell,R.H. and Johnson,P.R.
  TITLE     Molecular cloning of feline immunodeficiency virus
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 86, 2448-2452 (1989)
  STANDARD  full automatic
REFERENCE   2  (bases 1 to 9472)
  AUTHORS   Talbott,R.L., Sparger,E.E., Lovelace,K.M., Fitch,W.M.,
            Pedersen,N.C., Luciw,P.A. and Elder,J.H.
  TITLE     Nucleotide sequence and genomic organization of feline immuno-
            deficiency virus
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 86, 5743-5747 (1989)
  STANDARD  full automatic
REFERENCE   3  (bases 1 to 9474)
  AUTHORS   Olmstead,R.A., Hirsch,V.M., Purcell,R.H. and Johnson,P.R.
  TITLE     Nucleotide sequence analysis of feline immunodeficiency virus:
            genome organization and relationship to other lentiviruses
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 86, 8088-8096 (1989)
  STANDARD  full automatic
REFERENCE   4  (bases 7175 to 7182)
  AUTHORS   Elder,J.H.
  JOURNAL   Unpublished (1990)
  STANDARD  full automatic
COMMENT
            [4]  revision.
            [2] sequence kindly provided in computer-readable form by John
            Elder, Scripps Institute, La Jolla, CA. [3] sequence kindly
            provided in computer-readable form by Robert Olmstead, NIAID, NIH,
            Bethesda, MD. [1] contains the LTR sequence from the complete
            genome in [3].
            The sequence from [3] is shown (with differences from [2]); the
            substantive difference from [2] is at position 6123 where the
            latter possesses an early stop codon in orf2, which is thought to
            encode the transactivator protein. Clone 34TF10 is, nevertheless,
            biologically active as described in [2]. A difference earlier
            reported at 7175-7182 turns out to have been a typo [2,4].  Orf1 is
            weakly homologous (PLSEARCH) with the Q orf of visna and has no
            detectable similarity to the vif protein of HIV and SIV.  This is
            consistent with the clustering of FIV with the ungulate
            lentiviruses [2],[3]. Several 'urfs' are identified by [2] and [3]
            of which only orf3, possibly analogous to the L orf of visna, is
            annotated below; a small, possibly associated, orf is found at
            8955-9170. For a fuller discussion of the reading frames, see [2]
            and  [3].
FEATURES             Location/Qualifiers
     LTR             1..355
                     /standard_name="5' LTR"
     variation       replace(12,"t")
                     /citation=[3]
     variation       replace(12,"c")
                     /citation=[2]
     variation       replace(122,"t")
                     /citation=[2]
     variation       replace(122,"c")
                     /citation=[3]
     variation       replace(146,"t")
                     /citation=[2]
     variation       replace(146,"g")
                     /citation=[3]
     repeat_region   217..281
                     /note="R repeat 5' copy"
     misc_binding    357..377
                     /bound_moiety="unspecified"
                     /note="primer binding site"
     variation       373..374
                     /note="replace (373,'a')"
                     /citation=[2]
     variation       373..374
                     /note="replace (373,'ag')"
                     /citation=[3]
     variation       498..501
                     /note="ggac in [3]; agca in [2]"
     CDS             628..1980
                     /product="gag polyprotein"
                     /gene="gag"
                     /codon_start=1
                     /translation="MGNGQGRDWKMAIKRCSNVAVGVGGKSKKFGEGNFRWAIRMANV
                     STGREPGDIPETLDQLRLVICDLQERREKFGSSKEIDMAIVTLKVFAVAGLLNMTVST
                     AAAAENMYSQMGLDTRPSMKEAGGKEEGPPQAYPIQTVNGVPQYVALDPKMVSIFMEK
                     AREGLGGEEVQLWFTAFSANLTPTDMATLIMAAPGCAADKEILDESLKQLTAEYDRTH
                     PPDAPRPLPYFTAAEIMGIGLTQEQQAEARFAPARMQCRAWYLEALGKLAAIKAKSPR
                     AVQLRQGAKEDYSSFIDRLFAQIDQEQNTAEVKLYLKQSLSIANANADCKKAMSHLKP
                     ESTLEEKLRACQEIGSPGYKMQLLAEALTKVQVVQSKGSGPVCFNCKKPGHLARQCRE
                     VKKCNKCGKPGHVAAKCWQGNRKNSGNWKAGRAAAPVNQMQQAVMPSAPPMEEKLLDL
                     "
     variation       1575
                     /note="g in [3]; a in [2]"
     variation       1840
                     /note="g in [3]; c in [2]"
     variation       1977
                     /note="a in [3]; g in [2]"
     variation       2028
                     /note="g in [3]; a in [2]"
     variation       2033
                     /note="c in [3]; t in [2]"
     CDS             2251..5243
                     /product="pol polyprotein"
                     /gene="pol"
                     /codon_start=3
                     /translation="VLEDNSLIQPLLGRDNMIKFNIRLVMAQISDKIPVVKVKMKDPN
                     KGPQIKQWPLTNEKIEALTEIVERLEREGKVKRADPNNPWNTPVFAIKKKSGKWRMLI
                     DFRELNKLTEKGAEVQLGLPHPAGLQIKKQVTVLDIGDAYFTIPLDPDYAPYTAFTLP
                     RKNNAGPGRRFVWCSLPQGWILSPLIYQSTLDNIIQPFIRQNPQLDIYQYMDDIYIGS
                     NLSKKEHKEKVEELRKLLLWWGFETPEDKLQEEPPYTWMGYELHPLTWTIQQKQLDIP
                     EQPTLNELQKLAGKINWASQAIPDLSIKALTNMMRGNQNLNSTRQWTKEARLEVQKAK
                     KAIEEQVQLGYYDPSKELYAKLSLVGPHQISYQVYQKDPEKILWYGKMSRQKKKAENT
                     CDIALRACYKIREESIIRIGKEPRYEIPTSREAWESNLINSPYLKAPPPEVEYIHAAL
                     NIKRALSMIKDAPIPGAETWYIDGGRKLGKAAKAAYWTDTGKWQVMELEGSNQKAEIQ
                     ALLLALKAGSEEMNIITDSQYVINIILQQPDMMEGIWQEVLEELEKKTAIFIDWVPGH
                     KGIPGNEEVDKLCQTMMIIEGDGILDKRSEDAGYDLLAAKEIHLLPGEVKVIPTGVKL
                     MLPKGYWGLIIGKSSIGSKGLDVLGGVIDEGYRGEIGVIMINVSRKSITLMERQKIAQ
                     LIILPCKHEVLEQGKVVMDSERGDNGYGSTGVFSSWVDRIEEAEINHEKFHSDPQYLR
                     TEFNLPKMVAEEIRRKCPVCRIRGEQVGGQLKIGPGIWQMDCTHFDGKIILVGIHVES
                     GYIWAQIISQETADCTVKAVLQLLSAHNVTELQTDNGPNFKNQKMEGVLNYMGVKHKF
                     GIPGNPQSQALVENVNHTLKVWIRKFLPETTSLDNALSLAVHSLNFKRRGRIGGMAPY
                     ELLAQQESLRIQDYFSAIPQKLQAQWIYYKDQKDKKWKGPMRVEYWGQGSVLLKDEEK
                     GYFLIPRRHIRRVPEPCALPEGDE"
                     /partial
     variation       2288
                     /note="a in [3]; g in [2]"
     variation       2464
                     /note="g in [3]; a in [2]"
     variation       2490
                     /note="c in [3]; t in [2]"
     variation       2580
                     /note="c in [3]; t in [2]"
     variation       2631
                     /note="t in [3]; c in [2]"
     variation       2735
                     /note="a in [3]; g in [2]"
     variation       3733
                     /note="a in [3]; g in [2]"
     variation       3743
                     /note="a in [3]; t in [2]"
     variation       3812
                     /note="g in [3]; a in [2]"
     variation       4202
                     /note="g in [3]; a in [2]"
     variation       4211
                     /note="c in [3]; t in [2]"
     variation       4271
                     /note="c in [3]; t in [2]"
     variation       4493
                     /note="a in [3]; g in [2]"
     variation       4540
                     /note="g in [3]; t in [2]"
     variation       4706
                     /note="c in [3]; t in [2]"
     variation       4891
                     /note="g in [3]; a in [2]"
     CDS             5236..5991
                     /note="ORF 1"
                     /codon_start=1
                     /translation="MSEEDWQVSRRLFAVLQGGVNSAMLYISRLPPDEREKYKKDFKK
                     RLFDTETGFIKRLRKAEGIKWSFHTRDYYIGYVREMVAGSTTSLSLRMYIYISNPLWH
                     SQYRPGLKNFNKEWPFVNMWIKTGFMWDDIEKQNICIGGEVSPGWGPGMVGIAIKAFS
                     CGERKIEATPVMIIRGEIDPKKWCGDCWNLMCLRNSPPKTLQRLAMLACGVPAKKWRG
                     CCNQRFVSPYRTPADLEVIQSKPSWNLLWSGEL"
     variation       5332
                     /note="g in [3]; a in [2]"
     variation       5497
                     /note="t in [3]; c in [2]"
     variation       5559
                     /note="t in [3]; c in [2]"
     variation       5986
                     /note="t in [3]; c in [2]"
     CDS             5992..6228
                     /note="ORF 2"
                     /codon_start=1
                     /translation="MEDIIVLFNRVTEKLEKELAIRIFVLAHQLERDKAIRLLQGLFW
                     RYRFKKPRVDYCLCWWCCKFYYWQLQSTLSITTA"
     variation       6000
                     /note="c in [3]; a in [2]"
     variation       6123
                     /note="g in [3]; a in [2]"
     variation       6149
                     /note="t in [3]; c in [2]"
     variation       6179
                     /note="a in [3]; g in [2]"
     variation       6238
                     /note="g in [3]; t in [2]"
     variation       6245..6248
                     /note="attt in [3]; ttt in [2]"
     CDS             6266..8836
                     /product="envelope polyprotein"
                     /gene="env"
                     /codon_start=1
                     /translation="MAEGFAANRQWIGLEEAEELLDFDIATQMSEEGPLNPGVNPFRV
                     PGITEKEKQNYCNILQPKLQDLRNEIQEVKLEEGNAGKFRRARFLRYSDESVLSLVHA
                     FIGYCIYLGNRNKLGSLRHDIDIEAPQEECYNNREKGTTDNIKYGRRCCLGTVTLYLI
                     LFIGIIIYSQTTNAQVVWRLPPLVVPVEESEIIFWDCWAPEEPACQDFLGAMIHLKAK
                     TNISIREGPTLGNWAREIWATLFKKATRQCRRGRIWKRWNETITGPSGCANNTCYNVS
                     VIVPDYQCYLDRVDTWLQGKINISLCLTGGKMLYNKVTKQLSYCTDPLQIPLINYTFG
                     PNQTCMWNTSQIQDPEIPKCGWWNQMAYYNSCKWEEAKVKFHCQRTQSQPGSWFRAIS
                     SWKQRNRWEWRPDFESKKVKISLQCNSTKNLTFAMRSSGDYGEVTGAWIEFGCHRNKS
                     KLHAEARFRIRCRWNVGSNTSLIDTCGNTQKVSGANPVDCTMYSNKMYNCSLQNGFTM
                     KVDDLIMHFNMKKAVEMYNIAGNWSCTSDLPSSWGYMNCNCTNSSSSYSGTKMACPSN
                     RGILRNWYNPVAGLRQSLEQYQVVKQPDYLVVPEEVMEYKPRRKRAAIHVMLALAAVL
                     SIAGAGTGATAIGMVTQYHQVLATHQEAVEKVTEALKINNLRLVTLEHQVLVIGLKVE
                     AMEKFLYTAFAMQELGCNQNQFFCKIPPELWTRYNMTINQTIWNHGNITLGEWYNQTK
                     DLQQKFYEIIMDIEQNNVQGKKGIQQLQKWEDWVGWIGNIPQYLKGLLGGILGIGLGV
                     LLLILCLPTLVDCIRNCIHKILGYTVIAMPEVEGEEIQPQMELRRNGRQCGMSEKEEE
                     "
     variation       6306
                     /note="t in [3]; c in [2]"
     variation       6430
                     /note="c in [3]; t in [2]"
     variation       6548
                     /note="a in [3]; c in [2]"
     CDS             6712..6918
                     /note="ORF 3"
                     /codon_start=1
                     /translation="MLPRNGDFVPDFIYRNNNIFTDNQRSGSMETSTISSPSRRIRNN
                     FLGLLGTRRTRLSGLSWGNDTSKS"
     variation       6739
                     /note="c in [3]; t in [2]"
     variation       6753
                     /note="t in [3]; c in [2]"
     variation       6758
                     /note="a in [3]; g in [2]"
     variation       6764
                     /note="a in [3]; g in [2]"
     variation       6779
                     /note="a in [3]; g in [2]"
     variation       6782..6783
                     /note="aa in [3]; gg in [2]"
     variation       7175..7182
                     /note="acaggagg in [3]; caggagga in [2]"
     variation       7381
                     /note="a in [3]; g in [2]"
     variation       7484
                     /note="g in [3]; a in [2]"
     variation       7512..7513
                     /note="ag in [3]; ca in [2]"
     variation       7618
                     /note="a in [3]; c in [2]"
     variation       7625
                     /note="g in [3]; a in [2]"
     variation       7670
                     /note="a in [3]; g in [2]"
     variation       7704..7708
                     /note="aaaaa in [3]; caaat in [2]"
     variation       7750
                     /note="t in [3]; c in [2]"
     variation       7808..7810
                     /note="atg in [3]; gta in [2]"
     variation       7824..7828
                     /note="aaaag in [3]; caaaa in [2]"
     variation       7996
                     /note="g in [3]; a in [2]"
     variation       8054
                     /note="g in [3]; c in [2]"
     variation       8129
                     /note="g in [3]; a in [2]"
     variation       8222
                     /note="g in [3]; a in [2]"
     variation       8238
                     /note="a in [3]; g in [2]"
     variation       8329
                     /note="g in [3]; a in [2]"
     variation       8377
                     /note="t in [3]; c in [2]"
     variation       8394
                     /note="c in [3]; t in [2]"
     variation       8548..8550
                     /note="gaa in [3]; aac in [2]"
     variation       8588
                     /note="g in [3]; a in [2]"
     LTR             9120..9474
                     /note="3' LTR"
     variation       9131
                     /note="t in [3]; c in [2]"
     variation       9241
                     /note="c in [3]; t in [2]"
     variation       9265
                     /note="g in [3]; t in [2]"
     repeat_region   9336..9400
                     /note="R repeat 3' copy"
BASE COUNT     3571 a   1370 c   2119 g   2414 t
ORIGIN      5' terminus of 5' LTR
        1 tgggatgagt attggaaccc tgaagaaata gaaagaatgc ttatggacta gggactgttt
       61 acgaacaaat gataaaagga aatagctgag catgactcat agttaaagcg ctagcagctg
      121 cctaaccgca aaaccacatc ctatggaaag cttgctaatg acgtataagt tgttccattg
      181 taagagtata taaccagtgc tttgtgaaac ttcgaggagt ctctttgttg aggacttttg
      241 agttctccct tgaggctccc acagatacaa taaatatttg agattgaacc ctgtcgagta
      301 tctgtgtaat cttttttacc tgtgaggtct cggaatccgg gccgagaact tcgcagttgg
      361 cgcccgaaca gggacttgat tgagagtgat tgaggaagtg aagctagagc aatagaaagc
      421 tgttaagcag aactcctgct gacctaaata gggaagcagt agcagacgct gctaacagtg
      481 agtatctcta gtgaagcgga ctcgagctca taatcaagtc attgtttaaa ggcccagata
      541 aattacatct ggtgactctt cgcggacctt caagccagga gattcgccga gggacagtca
      601 acaaggtagg agagattcta cagcaacatg gggaatggac aggggcgaga ttggaaaatg
      661 gccattaaga gatgtagtaa tgttgctgta ggagtagggg ggaagagtaa aaaatttgga
      721 gaagggaatt tcagatgggc cattagaatg gctaatgtat ctacaggacg agaacctggt
      781 gatataccag agactttaga tcaactaagg ttggttattt gcgatttaca agaaagaaga
      841 gaaaaatttg gatctagcaa agaaattgat atggcaattg tgacattaaa agtctttgcg
      901 gtagcaggac ttttaaatat gacggtgtct actgctgctg cagctgaaaa tatgtattct
      961 caaatgggat tagacactag gccatctatg aaagaagcag gtggaaaaga ggaaggccct
     1021 ccacaggcat atcctattca aacagtaaat ggagtaccac aatatgtagc acttgaccca
     1081 aaaatggtgt ccatttttat ggaaaaggca agagaaggac taggaggtga ggaagttcaa
     1141 ctatggttta ctgccttctc tgcaaattta acacctactg acatggccac attaataatg
     1201 gccgcaccag ggtgcgctgc agataaagaa atattggatg aaagcttaaa gcaactgaca
     1261 gcagaatatg atcgcacaca tccccctgat gctcccagac cattacccta ttttactgca
     1321 gcagaaatta tgggtatagg attaactcaa gaacaacaag cagaagcaag atttgcacca
     1381 gctaggatgc agtgtagagc atggtatctc gaggcattag gaaaattggc tgccataaaa
     1441 gctaagtctc ctcgagctgt gcagttaaga caaggagcta aggaagatta ttcatccttt
     1501 atagacagat tgtttgccca aatagatcaa gaacaaaata cagctgaagt taagttatat
     1561 ttaaaacagt cattgagcat agctaatgct aatgcagact gtaaaaaggc aatgagccac
     1621 cttaagccag aaagtaccct agaagaaaag ttgagagctt gtcaagaaat aggctcacca
     1681 ggatataaaa tgcaactctt ggcagaagct cttacaaaag ttcaagtagt gcaatcaaaa
     1741 ggatcaggac cagtgtgttt taattgtaaa aaaccaggac atctagcaag acaatgtaga
     1801 gaagtgaaaa aatgtaataa atgtggaaaa cctggtcatg tagctgccaa atgttggcaa
     1861 ggaaatagaa agaattcggg aaactggaag gcggggcgag ctgcagcccc agtgaatcaa
     1921 atgcagcaag cagtaatgcc atctgcacct ccaatggagg agaaactatt ggatttataa
     1981 attataataa agtaggtact actacaacat tagaaaagag gccagaaata ctcatatttg
     2041 taaatggata tcctataaaa tttttattag acacaggagc agatataaca attttaaata
     2101 ggagagattt tcaagtaaaa aattctatag aaaatggaag gcaaaatatg attggagtag
     2161 gaggaggaaa gagaggaaca aattatatta atgtacattt agagattaga gatgaaaatt
     2221 ataagacaca atgtatattt ggtaatgttt gtgtcttaga agataactca ttaatacaac
     2281 cattattagg gagagataat atgattaaat tcaatattag gttagtaatg gctcaaattt
     2341 ctgataagat tccagtagta aaagtaaaaa tgaaggatcc taataaagga cctcaaataa
     2401 aacaatggcc attaacaaat gaaaaaattg aagccttaac agaaatagta gaaagactag
     2461 aaagagaagg gaaagtaaaa agagcagatc caaataatcc atggaataca ccagtatttg
     2521 ctataaaaaa gaaaagtgga aaatggagaa tgctcataga ttttagagaa ttaaacaaac
     2581 taactgagaa aggagcagag gtccagttgg gactacctca tcctgctggt ttacaaataa
     2641 aaaaacaagt aacagtatta gatatagggg atgcatattt caccattcct cttgatccag
     2701 attatgctcc ttatacagca tttactttac ctagaaaaaa taatgcggga ccaggaagga
     2761 gatttgtgtg gtgtagtcta ccacaaggct ggattttaag tccattgata tatcaaagta
     2821 cattagataa tataatacaa ccttttatta gacaaaatcc tcaattagat atttaccaat
     2881 atatggatga catttatata ggatcaaatt taagtaaaaa ggagcataaa gaaaaggtag
     2941 aagaattaag aaaattacta ttatggtggg gatttgaaac tccagaagat aaattacagg
     3001 aagaaccccc atatacatgg atgggttatg aattacatcc attaacatgg acaatacaac
     3061 agaaacagtt agacattcca gaacagccca ctctaaatga gttgcaaaaa ttagcaggaa
     3121 aaattaattg ggctagccaa gctattccag acttgagtat aaaagcatta actaacatga
     3181 tgagaggaaa tcaaaaccta aattcaacaa gacaatggac taaagaagct cgactggaag
     3241 tacaaaaggc aaaaaaggct atagaagaac aagtacaact aggatactat gaccccagta
     3301 aggagttata tgctaaatta agtttggtgg gaccacatca aataagttat caagtatatc
     3361 agaaggatcc agaaaagata ctatggtatg gaaaaatgag tagacaaaag aaaaaggcag
     3421 aaaatacatg tgatatagcc ttaagagcat gctataagat aagagaagag tctattataa
     3481 gaataggaaa agaaccaaga tatgaaatac ctacttctag agaagcctgg gaatcaaatt
     3541 taattaattc accatatctt aaggccccac ctcctgaggt agaatatatc catgctgctt
     3601 tgaatataaa gagagcgtta agtatgataa aagatgctcc aataccagga gcagaaacat
     3661 ggtatataga tggaggtaga aagctaggaa aagcagcaaa agcagcctat tggacagata
     3721 caggaaagtg gcaagtgatg gaattagaag gcagtaatca gaaggcagaa atacaagcat
     3781 tattattggc attaaaagca ggatcagagg agatgaatat tataacagat tcacaatatg
     3841 ttataaatat tattcttcaa caaccagata tgatggaggg aatctggcaa gaagttttag
     3901 aagaattgga gaagaaaaca gcaatattta tagattgggt cccaggacat aaaggtattc
     3961 caggaaatga ggaagtagat aagctttgtc aaacaatgat gataatagaa ggggatggga
     4021 tattagataa aaggtcagaa gatgcaggat atgatttatt agctgcaaaa gaaatacatt
     4081 tattgccagg agaggtaaaa gtaataccaa caggggtaaa gctaatgttg cctaaaggat
     4141 attggggatt aataatagga aaaagctcga tagggagtaa aggattggat gtattaggag
     4201 gggtaataga cgaaggatat cgaggtgaaa ttggagtaat aatgattaat gtatcaagaa
     4261 aatcaatcac cttaatggaa cgacaaaaga tagcacaatt aataatattg ccttgtaaac
     4321 atgaagtatt agaacaagga aaagtagtaa tggattcaga gagaggagac aatggttatg
     4381 ggtcaacagg agtattctcc tcttgggttg acagaattga ggaagcagaa ataaatcatg
     4441 aaaaatttca ctcagatcca cagtacttaa ggactgaatt taatttacct aaaatggtag
     4501 cagaagagat aagacgaaaa tgcccagtat gcagaatcag aggagaacaa gtgggaggac
     4561 aattgaaaat agggcctggt atctggcaaa tggattgcac acactttgat ggcaaaataa
     4621 ttcttgtggg tatacatgtg gaatcaggat atatatgggc acaaataatt tctcaagaaa
     4681 ctgctgactg tacagttaaa gctgtcttac aattgttgag tgctcataat gttactgaat
     4741 tacaaacaga taatggacca aattttaaaa atcaaaagat ggaaggagta ctcaattaca
     4801 tgggtgtgaa acataagttt ggtatcccag ggaacccaca gtcacaagca ttagttgaaa
     4861 atgtaaatca tacattaaaa gtttggattc ggaaattttt gcctgaaaca acctccttgg
     4921 ataatgcctt atctctcgct gtacatagtc tcaattttaa aagaagaggt aggataggag
     4981 ggatggcccc ttatgaatta ttagcacaac aagaatcctt aagaatacaa gattattttt
     5041 ctgcaatacc acaaaaattg caagcacagt ggatttatta taaagatcaa aaagataaga
     5101 aatggaaagg accaatgaga gtagaatact ggggacaggg atcagtatta ttaaaggatg
     5161 aagagaaggg atattttctt atacctagga gacacataag gagagttcca gaaccctgcg
     5221 ctcttcctga aggggatgag tgaagaagat tggcaggtaa gtagaagact ctttgcagtg
     5281 ctccaaggag gagtaaatag cgctatgcta tacatatcta ggctacctcc ggatgaaaga
     5341 gaaaagtata aaaaagactt caagaaaaga ctttttgaca cagaaacagg atttataaag
     5401 agactacgga aagctgaagg aataaaatgg agctttcata ctagagatta ttacatagga
     5461 tatgtcagag aaatggtggc aggatccact acatcattaa gtctaaggat gtatatatat
     5521 ataagtaacc cactatggca ttctcagtat cgtccaggtt tgaaaaattt caataaggaa
     5581 tggccttttg taaatatgtg gataaaaaca ggatttatgt gggatgatat tgaaaaacaa
     5641 aatatttgta taggaggaga agtttcacca ggatggggac cagggatggt aggtatagca
     5701 ataaaagctt ttagttgtgg cgaaagaaag attgaggcta ctcctgtaat gattataaga
     5761 ggagaaatag atccaaaaaa atggtgcgga gattgttgga atttaatgtg tcttagaaac
     5821 tcacctccaa agactttaca aagactcgct atgttggcgt gtggcgtgcc ggctaagaag
     5881 tggcgaggat gctgtaatca acgctttgtt tctccttaca gaacgcctgc tgatttagag
     5941 gtcattcaat ccaagcccag ctggaacctg ttatggtcgg gagaattatg aatggaagac
     6001 ataatagtat tattcaatag ggtcactgag aaactagaaa aagaattagc tatcagaata
     6061 tttgtattag cacatcaatt agaaagggac aaagctatta gattactaca aggattattt
     6121 tggagatata gatttaagaa accccgagta gattattgtt tatgttggtg gtgttgcaaa
     6181 ttctattatt ggcagttgca atctacatta tcaataacta ctgcttagaa atatttagat
     6241 taatatttca tttgcaacaa taagaatggc agaaggattt gcagccaata gacaatggat
     6301 aggactagaa gaagctgaag agttattaga ttttgatata gcaacacaaa tgagtgaaga
     6361 aggaccacta aatccaggag taaacccatt tagggtacct ggaataacag aaaaagaaaa
     6421 gcaaaactac tgtaacatat tacaacctaa gttacaagat ctaaggaacg aaattcaaga
     6481 ggtaaaactg gaagaaggaa atgcaggtaa gtttagaaga gcaagatttt taaggtattc
     6541 tgatgaaagt gtattgtccc tggttcatgc gttcatagga tattgtatat atttaggtaa
     6601 tcgaaataag ttaggatctt taagacatga cattgatata gaagcacccc aagaagagtg
     6661 ttataataat agagagaagg gtacaactga caatataaaa tatggtagac gatgttgcct
     6721 aggaacggtg actttgtacc tgattttatt tataggaata ataatatatt cacagacaac
     6781 caacgctcag gtagtatgga gacttccacc attagtagtc ccagtagaag aatcagaaat
     6841 aattttttgg gattgttggg caccagaaga acccgcctgt caggactttc ttggggcaat
     6901 gatacatcta aaagctaaga caaatataag tatacgagag ggacctacct tggggaattg
     6961 ggctagagaa atatgggcaa cattattcaa aaaggctact agacaatgta gaagaggcag
     7021 aatatggaaa agatggaatg agactataac aggaccatca ggatgtgcta ataacacatg
     7081 ttataatgtt tcagtaatag tacctgatta tcagtgttat ttagatagag tagatacttg
     7141 gttacaaggg aaaataaata tatcattatg tctaacagga ggaaaaatgt tgtacaataa
     7201 agttacaaaa caattaagct attgtacaga cccattacaa atcccactga tcaattatac
     7261 atttggacct aatcaaacat gtatgtggaa tacttcacaa attcaggacc ctgaaatacc
     7321 aaaatgtgga tggtggaatc aaatggccta ttataacagt tgtaaatggg aagaggcaaa
     7381 agtaaagttt cattgtcaaa gaacacagag tcagcctgga tcatggttta gagcaatctc
     7441 gtcatggaaa caaagaaata gatgggagtg gagaccagat tttgaaagta aaaaggtgaa
     7501 aatatctcta cagtgcaata gcacaaaaaa cctaaccttt gcaatgagaa gttcaggaga
     7561 ttatggagaa gtaacgggag cttggataga gtttggatgt catagaaata aatcaaaact
     7621 tcatgctgaa gcaaggttta gaattagatg tagatggaat gtagggagta atacctcgct
     7681 cattgataca tgtggaaaca ctcaaaaagt ttcaggtgcg aatcctgtag attgtaccat
     7741 gtattcaaat aaaatgtaca attgttcttt acaaaacggg tttactatga aggtagatga
     7801 ccttattatg catttcaata tgaaaaaggc tgtagaaatg tataatattg ctggaaattg
     7861 gtcttgtaca tctgacttgc catcgtcatg ggggtatatg aattgtaatt gtacaaatag
     7921 tagtagtagt tatagtggta ctaaaatggc atgtcctagc aatcgaggca tcttaaggaa
     7981 ttggtataac ccagtggcag gattacgaca atccttagaa cagtatcaag ttgtaaaaca
     8041 accagattac ttagtggtcc cagaggaagt catggaatat aaacctagaa ggaaaagggc
     8101 agctattcat gttatgttgg ctcttgcagc agtattatct attgccggtg cagggacggg
     8161 ggctactgct atagggatgg taacacaata ccaccaagtt ctggcaaccc atcaagaagc
     8221 tgtagaaaag gtgactgaag ccttaaagat aaacaactta agattagtta cattagagca
     8281 tcaagtacta gtaataggat taaaagtaga agctatggaa aaatttttgt atacagcttt
     8341 cgctatgcaa gaattaggat gtaatcaaaa tcaatttttc tgcaaaatcc ctcctgagtt
     8401 gtggacaagg tataatatga ctataaatca aacaatatgg aatcatggaa atataacttt
     8461 gggggaatgg tataaccaaa caaaagattt acaacaaaag ttttatgaaa taataatgga
     8521 catagaacaa aataatgtac aagggaagaa agggatacaa caattacaaa agtgggaaga
     8581 ttgggtagga tggataggaa atattccaca atatttaaag ggactattgg gaggtatctt
     8641 gggaatagga ttaggagtgt tattattgat tttatgttta cctacattgg ttgattgtat
     8701 aagaaattgt atccacaaga tactaggata cacagtaatt gcaatgcctg aagtagaagg
     8761 agaagaaata caaccacaaa tggaattgag gagaaatggt aggcaatgtg gcatgtctga
     8821 aaaagaggag gaatgatgaa gtatctcaga cttattttat aagggagata ctgtgctgag
     8881 ttcttccctt tgaggaaggt atgtcatatg aatccatttc gaatcaaatc aaactaataa
     8941 agtatgtatt gtaaggtaaa aggaaaagac aaagaagaag aagaaagaag aaagccttca
     9001 agaggatgat gacagagtta gaagatcgct tcaggaagct atttggcacg acttctacaa
     9061 cgggagacag cacagtagat tctgaagatg aacctcctaa aaaagaaaaa agggtggact
     9121 gggatgagta ttggaaccct gaagaaatag aaagaatgct tatggactag ggactgttta
     9181 cgaacaaatg ataaaaggaa atagctgagc atgactcata gttaaagcgc tagcagctgc
     9241 ctaaccgcaa aaccacatcc tatggaaagc ttgctaatga cgtataagtt gttccattgt
     9301 aagagtatat aaccagtgct ttgtgaaact tcgaggagtc tctttgttga ggacttttga
     9361 gttctccctt gaggctccca cagatacaat aaatatttga gattgaaccc tgtcgagtat
     9421 ctgtgtaatc ttttttacct gtgaggtctc ggaatccggg ccgagaactt cgca
//