From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: S.pneumoniae (56742) ponA gene for penicillin-binding protein 1a
Date: 28 Dec 92 21:46:12 GMT
Distribution: bionet
Checksum: 58706 5

LOCUS       STRPONA4     2157 bp ds-DNA             BCT       02-DEC-1992
DEFINITION  S.pneumoniae (56742) ponA gene for penicillin-binding protein 1a
ACCESSION   X67869
KEYWORDS    penicillin-binding protein 1a; ponA gene.
SOURCE      Streptococcus pneumoniae DNA.
  ORGANISM  Streptococcus pneumoniae
            Prokaryotae; Firmicutes; Firmibacteria; Gram-positive cocci;
            Streptococcaceae.
REFERENCE   1  (bases 1 to 2157)
  AUTHORS   Martin,C., Sibold,C. and Hakenbeck,R.
  TITLE     Relatedness of penicillin-binding protein 1a genes from different
            clones of penicillin-resistant Streptococcus pneumoniae isolated in
            South Africa and Spain
  JOURNAL   EMBO J. 11, 3831-3836 (1992)
  STANDARD  full automatic
COMMENT     *source: strain=56742; *source: cell_line=plant cultivar; *source:
            clone=pcMK1-pcMK16;
               From EMBL    entry SPPONA4;  dated 21-OCT-1992.
FEATURES             Location/Qualifiers
     CDS             1..>2157
                     /gene="ponA"
                     /product="penicillin-binding protein1a"
                     /codon_start=1
                     /translation="MNKPTILRLIKYLSISFLSLVIAAIVLGGGVFFYYVSKAPSLSE
                     SKLVATTSSKIYDNKNQLIADLGSERRVNAQANDIPTDLVKAIVSIEDHRFFDHRGID
                     SIRILGAFLRNLQSNSLQGGSTLTQQLIKLTYFSTSTSDQTISRKAQEAWLAIQLEQK
                     ATKQEILTYYINKVYMSNGNYGMQTAAQNYYGKDLNNLSLPQLALLAGMPQAPNQYDP
                     YSHPEAAQDRRNLVLSEMKNQGYISAEQYEKAVNTPITDGLQSLKSASNYPAYMDNYL
                     KEVINQVEQETGYNLLTTGMDVYTNVDQEAQKHLWDIYNSDQYVSYPDDELQVASTVV
                     DVSNGKVIAQLGARHQASNVSFGTNQAVETNRDWGSAMKPITDYAPAIEYGVYDSTAT
                     MVNDIPYNYPGTSTPVYNWDRAYFGNITLQYALQQSRNVTAVETLNKVGLDRAKTFLN
                     GLGIDYPSMHYANAISSNTTESNKQYGASSEKMAAAYAAFANGGTYYKPMYIHKVVFS
                     DGSKKEFSNVGTRAMKETTAYMMTDMMKTVLTYGTGRGAYLPWLPQAGKTGTSNYTDE
                     EVENHIKNTGYVAPDEMFVGYTRKYSMAVWTGYSNRLTPIVGDGFLVAAKVYRSMITY
                     LSEDTHPEDWTMPDGLFRNGEFVFKNGARPIWTEPSTQQSSTAESSSSSSDSSTSQSS
                     STTPSTNNSTTTNPNNNTQQSNTTPDQQNQNPQPAQP"
BASE COUNT      696 a    494 c    403 g    564 t
ORIGIN      
        1 atgaacaaac caacgattct gcgcctaatc aagtatctga gcattagctt cttaagcttg
       61 gttatcgcag ccattgtctt aggcggagga gtttttttct actacgttag caaggctcct
      121 agcctatccg agagtaaact agttgcaaca acttctagta aaatctacga caataaaaat
      181 caactcattg ctgacttggg ttctgaacgc cgcgtcaatg cccaagctaa tgatattccc
      241 acagatttgg ttaaggcaat cgtttctatc gaagaccatc gcttcttcga ccacaggggg
      301 attgattcca tccgtatcct gggagctttc ttgcgcaatc tgcaaagtaa ttccctccaa
      361 ggtggatcaa ctctcaccca acagttgatt aagttgactt acttttcaac ctcgacttcc
      421 gaccagacta tttctcgtaa ggctcaggaa gcttggttag cgattcagtt agaacaaaaa
      481 gcaaccaaac aggaaatctt gacctactat ataaataagg tctacatgtc taatggcaac
      541 tatggaatgc agacagcagc tcaaaactac tatggtaaag acctcaataa tttaagttta
      601 cctcagttag ccttgctggc tggaatgcct caggcaccaa accaatatga cccctattca
      661 catccagaag cagcccaaga ccgccgaaac ttggtcttat ctgaaatgaa aaatcaaggt
      721 tacatctctg ctgaacagta tgagaaagca gtcaatacac caattactga tggactacaa
      781 agtctcaaat cagcaagtaa ttaccctgct tacatggata attacctcaa ggaggtcatc
      841 aatcaagtag aacaagaaac tggctataac cttctaacta ctgggatgga tgtttacaca
      901 aatgtagacc aagaagctca aaaacatctg tgggatatct acaactccga tcaatacgtc
      961 tcttaccctg acgatgaatt gcaagtcgca tctacggtcg tagatgtttc aaatggtaaa
     1021 gtcatcgccc aacttggagc tcgtcaccaa gcaagtaacg tttcatttgg taccaaccaa
     1081 gctgtggaaa ccaatcgtga ctggggttct gctatgaaac caatcaccga ttatgcacct
     1141 gccatagaat acggtgttta tgattccact gcaactatgg ttaatgatat tccttataac
     1201 tatccgggaa caagcacacc tgtctacaac tgggatagag catatttcgg taatattact
     1261 ctgcaatatg ctcttcaaca atcacgaaat gtcacagccg ttgagacttt gaataaggtc
     1321 ggtctagata gagctaaaac cttccttaat ggtcttggta tcgactatcc aagcatgcat
     1381 tatgcaaacg ccatttcaag taatacaaca gaatctaata aacaatacgg agcaagtagt
     1441 gaaaaaatgg ctgctgctta tgctgccttt gcaaatggtg gcacttacta taaaccaatg
     1501 tatatccata aagtcgtctt cagtgatgga agtaaaaaag agttctctaa tgtcggaact
     1561 cgtgccatga aggaaacgac agcctatatg atgaccgaca tgatgaaaac agtcttgact
     1621 tatggaactg ggcgtggtgc ctatcttcct tggcttcctc aagctggtaa aacaggaacc
     1681 tctaactata cagatgagga agttgaaaac cacatcaaga acactggcta tgtagctcca
     1741 gatgaaatgt ttgttggtta tactcgtaag tattctatgg ctgtatggac aggttattcg
     1801 aatcgtttaa ctcctatcgt tggagatggt ttcctagttg cagctaaagt ttatcgctca
     1861 atgataacgt atctatcaga agatactcat ccagaagact ggacgatgcc agacggactt
     1921 ttcagaaacg gggaatttgt attcaaaaat ggagctcgcc caatatggac tgaaccctct
     1981 actcaacaat cctcaacagc tgaaagttca agctcatcat cagatagttc aacttcacag
     2041 tctagctcaa ccactccaag cacaaataat agtacgacta ccaatcctaa caataatacg
     2101 caacaatcaa atacaacccc tgatcaacaa aatcagaatc ctcaaccagc acaacca
//