From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Pseudomonas putida dihydro-orotase (pyrC) gene, complete cds.
Date: 28 Aug 92 10:01:05 GMT
Distribution: bionet
Checksum: 00766     4

LOCUS       PSEPYRC      1275 bp ds-DNA             BCT       28-AUG-1992
DEFINITION  Pseudomonas putida dihydro-orotase (pyrC) gene, complete cds.
ACCESSION   M97254
KEYWORDS    aspartate carbamoyltransferase; dihydro-orotase; pyrB gene;
            pyrC gene.
SOURCE      Pseudomonas putida (strain PpN) DNA.
  ORGANISM  Pseudomonas putida
            Prokaryotae; Gracilicutes; Scotobacteria; Pseudomonadaceae.
REFERENCE   1  (bases 1 to 1275)
  AUTHORS   Schurr,M.J., Vickrey,J.F., Benjamin,R.C., Shanley,M.S., Cunin,R.
            and O'Donovan,G.A.
  TITLE     Cloning and Nucleotide Sequence of pyrBC genes of P. putida
            encoding Aspartate transcarbamoylase and Dihydroorotase
  JOURNAL   Unpublished (1992)
  STANDARD  full staff_entry
FEATURES             Location/Qualifiers
     CDS             1..1275
                     /EC_number="3.5.2.3"
                     /product="dihydro-orotase"
                     /gene="pyrC"
                     /codon_start=1
                     /translation="MTISILGARVIDPKTGLDQVTDLHLDGGRIAAIGAAPAGFSASR
                     TIQADGVVAAPGLVDLGVSLREPGYSRKGNIRSETRAAVAGGVTSLCCPPQTRPVLDT
                     LAVAELILDRAREAANSKVYPIGALTKGLEGEQLAELVALRDTGCVAFGNGLKQIPNN
                     RTLARALEYAATFDLTVVFHSQDRDLAEGGLAHEGAMASFLGLPGIPESAETVALARN
                     LLLVEQSGVRAHFSQITSARGAQLIAQAQELGLPVTADVALYQLILTDESVRQFSSLY
                     HVQPPLRTAKDRDGLRAAVKSGVIQAISSHHQPHERDAKLAPFGATEPGISSVELLLP
                     LAMTLVQDGLLDLPTLLARLSSGPAAALRVPAGELKVGGAADLVLFDPQASTVAGEQW
                     SSRGENCPFIGHCLPGAVRYTLVDGHVCHGPE"
     misc_feature    2..5
                     /note="stop codon for the P. putida pyrB, pyrB and pyrC
                     overlap by 4 bp"
BASE COUNT      204 a    458 c    415 g    198 t
ORIGIN
        1 gtgaccatca gtattcttgg cgcccgggtc atcgatccca aaaccggcct ggaccaggtc
       61 accgacctgc acctggacgg tggccgcatt gccgccatcg gcgccgcgcc agccggcttc
      121 agcgccagcc gcacgatcca ggccgatggc gtggttgccg caccgggcct ggtggacctg
      181 ggcgtgtccc tgcgcgagcc gggttacagc cgcaaaggca acatccgcag tgaaacccgc
      241 gcagccgtgg ccggcggtgt caccagcctg tgctgcccgc cgcagaccag gccggtgctg
      301 gacaccctgg cggtggccga gctgatcctg gaccgcgccc gtgaggctgc caacagcaag
      361 gtgtacccga tcggcgccct gaccaaaggc cttgaaggcg agcaactggc cgagctggtg
      421 gccctgcgcg acaccggttg cgtggccttc ggcaacggcc tgaaacaaat ccccaacaac
      481 cgtaccctgg cccgcgccct ggaatacgcc gccaccttcg acctgaccgt ggtgttccac
      541 tcccaggacc gcgacctggc cgagggcggc ctggcccacg aaggtgccat ggccagtttc
      601 ctcggcctgc cgggcatccc ggaaagcgcc gaaaccgtgg cactggcgcg caacctgctg
      661 ctggtggaac aaagcggcgt gcgtgcgcac ttcagccaga tcaccagcgc ccgtggcgca
      721 cagctgattg cccaggcgca ggaactcggc ctgccggtga cggccgatgt agcgctgtac
      781 cagctgatac tcaccgatga gtccgtgcgg cagttttcca gcctgtacca cgtgcagccg
      841 ccgctgcgca ctgccaaaga ccgggatggc ctgcgcgctg cggtcaaatc gggcgtgatc
      901 caggcgattt ccagccacca ccagccccat gagcgcgacg ccaagctggc cccgttcggc
      961 gccaccgagc cgggtatcag cagcgtcgag ttgctgctgc cactggccat gaccctggta
     1021 caggacggcc tgctcgacct gccgaccttg ctcgcccgcc tgagcagcgg cccggctgcc
     1081 gccctgcgcg tgccggccgg tgagctgaag gtgggtggtg cggccgacct ggtactgttc
     1141 gacccgcaag cctccaccgt tgccggcgag cagtggtctt cgcgtggcga gaactgcccg
     1201 ttcatcggcc actgcctgcc gggtgcggtg cgttatacct tggtcgatgg ccacgtctgc
     1261 cacgggcctg agtaa
//