From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Murine nitric oxide synthase (MAC-NOS) gene, complete cds.
Date: 8 Apr 92 10:02:08 GMT
Distribution: bionet
Checksum: 10673     8

LOCUS       MUSNOSA      3991 bp ss-mRNA            ROD       08-APR-1992
DEFINITION  Murine nitric oxide synthase (MAC-NOS) gene, complete cds.
ACCESSION   M84373
KEYWORDS    nitric oxide synthase.
SOURCE      Mus musculus (library: lambda ZAPII) cDNA to mRNA.
  ORGANISM  Mus musculus
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Rodentia; Myomorpha; Muridae; Murinae.
REFERENCE   1  (bases 1 to 3991)
  AUTHORS   Cunningham,J.M., Orloff,G.J. and Lyons,C.R.
  TITLE     Molecular cloning and functional expression of an inducible nitric
            oxide synthase from a murine macrophage cell line
  JOURNAL   Unpublished (1992)
  STANDARD  simple staff_entry
FEATURES             Location/Qualifiers
     CDS             185..3619
                     /product="nitric oxide synthase"
                     /gene="MAC-NOS"
                     /codon_start=1
                     /translation="MACPWKFLFKVKSYQSDLKEEKDINNNVKKTPCAVLSPTIQDDP
                     KSHQNGSPQLLTGTAQNVPESLDKLHVTSTRPQYVRIKNWGSGEILHDTLHHKATSDF
                     TCKSKSCLGSIMNPKSLTRGPRDKPTPLEELLPHAIEFINQYYGSFKEAKIEEHLARL
                     EAVTKEIETTGTYQLTLDELIFATKMAWRNAPRCIGRIQWSNLQVFDARNCSTAQEMF
                     QHICRHILYATNNGNIRSAITVFPQRSDGKHDFRLWNSQLIRYAGYQMPDGTIRGDAA
                     TLEFTQLCIDLGWKPRYGRFDVLPLVLQADGQDPEVFEIPPDLVLEVTMEHPKYEWFQ
                     ELGLKWYALPAVANMLLEVGGLEFPACPFNGWYMGTEIGVRDFCDTQRYNILEEVGRR
                     MGLETHTLASLWKDRAVTEINVAVLHSFQKQNVTIMDHHTASESFMKHMQNEYRARGG
                     CPADWIWLVPPVSGSITPVFHQEMLNYVLSPFYYYQIEPWKTHIWQNEKLRPRRREIR
                     FRVLVKVVFFASMLMRKVMASRVRATVLFATETGKSEALARDLATLFSYAFNTKVVCM
                     DQYKASTLEEEQLLLVVTSTFGNGDCPSNGQTLKKSLFMLRELNHTFRYAVFGLGSSM
                     YPQFCAFAHDIDQKLSHLGASQLAPTGEGDELSGQEDAFRSWAVQTFRAACETFDVRS
                     KHHIQIPKRFTSNATWEPQQYRLIQSPEPLDLNRALSSIHAKNVFTMRLKSQQNLQSE
                     KSSRTTLLVQLTFEGSRGPSYLPGEHLGIFPGNQTALVQGILERVVDCPTPHQTVCLE
                     VLDESGSYWVKDKRLPPCSLSQALTYFLDITTPPTQLQLHKLARFATDETDRQRLEAL
                     CQPSEYNDWKFSNNPTFLEVLEEFPSLHVPAAFLLSQLPILKPRYYSISSSQDHTPSE
                     VHLTVAVVTYRTRDGQGPLHHGVCSTWIRNLKPQDPVPCFVRSVSGFQLPEDPSQPCI
                     LIGPGTGIAPFRSFWQQRLHDSQHKGLKGGRMSLVFGCRHPEEDHLYQEEMQEMVRKR
                     VLFQVHTGYSRLPGKPKVYVQDILQKQLANEVLSVLHGEQGHLYICGDVRMARDVATT
                     LKKLVATKLNLSEEQVEDYFFQLKSQKRYHEDIFGAVFSYGAKKGSALEEPKATRL"
BASE COUNT      952 a   1169 c   1017 g    853 t
ORIGIN
        1 aggccccacg ggacacagtg tcactggttt gaaacttctc agccaccttg gtgaagggac
       61 tgagctgtta gagacacttc tgaggctcct cacgcttggg tcttgttcac tccacggagt
      121 agcctagtca actgcaagag aacggagaac gttggatttg gagcagaagt gcaaagtctc
      181 agacatggct tgcccctgga agtttctctt caaagtcaaa tcctaccaaa gtgacctgaa
      241 agaggaaaag gacattaaca acaacgtgaa gaaaacccct tgtgctgttc tcagcccaac
      301 aatacaagat gaccctaaga gtcaccaaaa tggctccccg cagctcctca ctgggacagc
      361 acagaatgtt ccagaatccc tggacaagct gcatgtgaca tcgacccgtc cacagtatgt
      421 gaggatcaaa aactggggca gtggagagat tttgcatgac actcttcacc acaaggccac
      481 atcggatttc acttgcaagt ccaagtcttg cttggggtcc atcatgaacc ccaagagttt
      541 gaccagagga cccagagaca agcctacccc tctggaggag ctcctgcctc atgccattga
      601 gttcatcaac cagtattatg gctcctttaa agaggcaaaa atagaggaac atctggccag
      661 gctggaagct gtaacaaagg aaatagaaac aacaggaacc taccagctca ctctggatga
      721 gctcatcttt gccaccaaga tggcctggag gaatgcccct cgctgcatcg gcaggatcca
      781 gtggtccaac ctgcaggtct ttgacgctcg gaactgtagc acagcacagg aaatgtttca
      841 gcacatctgc agacacatac tttatgccac caacaatggc aacatcaggt cggccatcac
      901 tgtgttcccc cagcggagtg acggcaaaca tgacttcagg ctctggaatt cacagctcat
      961 ccggtacgct ggctaccaga tgcccgatgg caccatcaga ggggatgctg ccaccttgga
     1021 gttcacccag ttgtgcatcg acctaggctg gaagccccgc tatggccgct ttgatgtgct
     1081 gcctctggtc ttgcaagctg atggtcaaga tccagaggtc tttgaaatcc ctcctgatct
     1141 tgtgttggag gtgaccatgg agcatcccaa gtacgagtgg ttccaggagc tcgggttgaa
     1201 gtggtatgca ctgcctgccg tggccaacat gctactggag gtgggtggcc tcgaattccc
     1261 agcctgcccc ttcaatggtt ggtacatggg caccgagatt ggagttcgag acttctgtga
     1321 cacacagcgc tacaacatcc tggaggaagt gggccgaagg atgggcctgg agacccacac
     1381 actggcctcc ctctggaaag accgggctgt cacggagatc aatgtggctg tgctccatag
     1441 tttccagaag cagaatgtga ccatcatgga ccaccacaca gcctcagagt ccttcatgaa
     1501 gcacatgcag aatgagtacc gggcccgtgg aggctgcccg gcagactgga tttggctggt
     1561 ccctccagtg tctgggagca tcacccctgt gttccaccag gagatgttga actatgtcct
     1621 atctccattc tactactacc agatcgagcc ctggaagacc cacatctggc agaatgagaa
     1681 gctgaggccc aggaggagag agatccgatt tagagtcttg gtgaaagtgg tgttctttgc
     1741 ttccatgcta atgcgaaagg tcatggcttc acgggtcaga gccacagtcc tctttgctac
     1801 tgagacaggg aagtctgaag cactagccag ggacctggcc accttgttca gctacgcctt
     1861 caacaccaag gttgtctgca tggaccagta taaggcaagc accttggaag aggagcaact
     1921 actgctggtg gtgacaagca catttgggaa tggagactgt cccagcaatg ggcagactct
     1981 gaagaaatct ctgttcatgc ttagagaact caaccacacc ttcaggtatg ctgtgtttgg
     2041 ccttggctcc agcatgtacc ctcagttctg cgcctttgct catgacatcg accagaagct
     2101 gtcccacctg ggagcctctc agcttgcccc aacaggagaa ggggacgaac tcagtgggca
     2161 ggaggatgcc ttccgcagct gggctgtaca aaccttccgg gcagcctgtg agacctttga
     2221 tgtccgaagc aaacatcaca ttcagatccc gaaacgcttc acttccaatg caacatggga
     2281 gccacagcaa tataggctca tccagagccc ggagccttta gacctcaaca gagccctcag
     2341 cagcatccat gcaaagaacg tgtttaccat gaggctgaaa tcccagcaga atctgcagag
     2401 tgaaaagtcc agccgcacca ccctcctcgt tcagctcacc ttcgagggca gccgagggcc
     2461 cagctacctg cctggggaac acctggggat cttcccaggc aaccagaccg ccctggtgca
     2521 gggaatcttg gagcgagttg tggattgtcc tacaccacac caaactgtgt gcctggaggt
     2581 tctggatgag agcggcagct actgggtcaa agacaagagg ctgcccccct gctcactcag
     2641 ccaagccctc acctacttcc tggacattac gacccctccc acccagctgc agctccacaa
     2701 gctggctcgc tttgccacgg acgagacgga taggcagaga ttggaggcct tgtgtcagcc
     2761 ctcagagtac aatgactgga agttcagcaa caaccccacg ttcctggagg tgcttgaaga
     2821 gttcccttcc ttgcatgtgc ccgctgcctt cctgctgtcg cagctcccta tcttgaagcc
     2881 ccgctactac tccatcagct cctcccagga ccacaccccc tcggaggttc acctcactgt
     2941 ggccgtggtc acctaccgca cccgagatgg tcagggtccc ctgcaccatg gtgtctgcag
     3001 cacttggatc aggaacctga agccccagga cccagtgccc tgctttgtgc gaagtgtcag
     3061 tggcttccag ctccctgagg acccctccca gccttgcatc ctcattgggc ctggtacggg
     3121 cattgctccc ttccgaagtt tctggcagca gcggctccat gactcccagc acaaagggct
     3181 caaaggaggc cgcatgagct tggtgtttgg gtgccggcac ccggaggagg accacctcta
     3241 tcaggaagaa atgcaggaga tggtccgcaa gagagtgctg ttccaggtgc acacaggcta
     3301 ctcccggctg cccggcaaac ccaaggtcta cgttcaggac atcctgcaaa agcagctggc
     3361 caatgaggta ctcagcgtgc tccacgggga gcagggccac ctctacattt gcggagatgt
     3421 gcgcatggct cgggatgtgg ctaccacatt gaagaagctg gtggccacca agctgaactt
     3481 gagcgaggag caggtggaag actatttctt ccagctcaag agccagaaac gttatcatga
     3541 agatatcttc ggtgcagtct tttcctatgg ggcaaaaaag ggcagcgcct tggaggagcc
     3601 caaagccacg aggctctgac agcccagagt tccagcttct ggcactgagt aaagataatg
     3661 gtgaggggct tggggagaca gcgaaatgca acccccccca agcccctcat gtcattcccc
     3721 cctcctccac cctaccaagt agtattgtac tattgtggac tactaaatct ctctcctctc
     3781 ctccctcccc tctctccctt tcctcccttc ttctccactc cccagctccc tccttctcct
     3841 tctcctcctt tgcctctcac tcttccttgg agctgagagc agagaaaaac tcaacctcct
     3901 gactgaagca ctttgggtga ccaccaggag gcaccatgcc gccgctctaa tacttagctg
     3961 cactatgtac agatatttat acttcatatt t
//