From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: T.thermophilus pheS and pheT genes for phenylalanyl-tRNA synthetase
Date: 29 Jul 92 10:03:56 GMT
Distribution: bionet
Checksum: 13617     9

LOCUS       TTHPHEST     4459 bp ds-DNA             BCT       29-JUL-1992
DEFINITION  T.thermophilus pheS and pheT genes for phenylalanyl-tRNA synthetase
ACCESSION   X65609
KEYWORDS    phenylalanyl-trna synthetase.
SOURCE      Thermus thermophilus DNA.
  ORGANISM  Thermus thermophilus
            Prokaryotae; Gracilicutes; Scotobacteria; Neisseriaceae.
REFERENCE   1  (bases 1 to 4459)
  AUTHORS   Kreutzer,R., Kruft,V., Bobkova,E.V., Lavrik,O.J. and Sprinzl,M.
  TITLE     Structure and regulation of the Thermus thermophilus
            phenylalanyl-tRNA synthetase
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
REFERENCE   2  (bases 1 to 4459)
  AUTHORS   Kreutzer,R.
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
COMMENT     *source: strain=HB8; *source: clone=plasmid pPhiRS1;
            From EMBL    entry TTPHEST;  dated 19-MAY-1992.
FEATURES             Location/Qualifiers
     -35_signal      566..571
     -10_signal      592..597
     CDS             615..1667
                     /EC_number="6.1.1.20"
                     /product="phenylalanine--tRNA ligase"
                     /gene="pheS"
                     /codon_start=1
                     /translation="MLEEALAAIQNARDLEELKALKARYLGKKGLLTQEMKGLSALPL
                     EERRKRGQELNAIKAALEAALEAREKALEEAALKEALERERVDVSLPGASLFSGGLHP
                     ITLMERELVEIFRALGYQAVEGPEVESEFFNFDALNIPEHHPARDMWDTFWLTGEGFR
                     LEGPLGEEVEGRLLLRTHTSPMQVRYMVAHTPPFRIVVPGRVFRFEQTDATHEAVFHQ
                     LEGLVVGEGIAMAHLKGAIYELAQALFGPDSKVRFQPVYFPFVEPGAQFAVWWPEGGK
                     WLELGGAGMVHPKVFQAVDAYRERLGLPPAYRGVTGFAFGLGVERLAMLRYGIPDIRY
                     FFGGRLKFLEQFKGVL"
     CDS             1664..4021
                     /EC_number="6.1.1.20"
                     /product="phenylalanine--tRNA ligase"
                     /gene="pheT"
                     /codon_start=1
                     /translation="MRVPFSWLKAYVPELESPEVLEERLAGLGFETDRIERVFPIPRG
                     VVFARVLEAHPIPGTRLKRLVLDAGRTVEVVSGAENARKGIGVALALPGTELPGLGQK
                     VGERVIQGVRSFGMALSPRELGVGEYGGGLLEFPEDALPPGTPLSEAWPEEVVLDLEV
                     TPNRPDALGLLGLARDLHALGYALVEPEAALKAEALPLPFALKVEDPEGAPHFTLGYA
                     FGLRVAPSPLWMQRALFAAGMRPINNVVDVTNYVMLERAQPMHAFDLRFVGEGIAVRR
                     AREGERLKTLDGVERTLHPEDLVIAGWRGEESFPLGLAGVMGGAESEVREDTEAIALE
                     VACFDPVSIRKTARRHGLRTEASHRFERGVDPLGQVPAQRRALSLLQALAGARVAEAL
                     LEAGSPKPPEAIPFRPEYANRLLGTSYPEAEQIAILKRLGCRVEGEGPTYRVTPPSHR
                     LDLRLEEDLVEEVARIQGYETIPLALPAFFPAPDNRGVEAPYRKEQRLREVLSGLGFQ
                     EVYTYSFMDPEDARRFRLDPPRLLLLNPLAPEKAALRTHLFPGLVRVLKENLDLDRPE
                     RALLFEVGRVFREREETHLAGLLFGEGVGLPWAKERLSGYFLLKGYLEALFARLGLAF
                     RVEAQAFPFLHPGVSGRVLVEGEEVGFLGALHPEIAQELELPPVHLFELRLPLPDKPL
                     AFQDPSRHPAAFRDLAVVVPAPTPYGEVEALVREAAGPYLESLALFDLYQGPPLPEGH
                     KSLAFHLRFRHPKRTLRDEEVEEAVSRVAEALRARGFGLRGLDTP"
     terminator      4028..4056
BASE COUNT      632 a   1645 c   1520 g    662 t
ORIGIN
        1 ggatccccgt gggcgtcttc tggaaggccg agctgcccac ctttgaagcg cggcttaagg
       61 cctacctccc ccgctacccc gaggcctacc cggcccgggg tccggaggcc cccttggacc
      121 tggagggcct cctccaggag ttcgccctct aaagctggaa ccgggccgcc ccttccttcg
      181 ggcggtaagc cgggtccagg aagcgcccgg gggcgaaggc ccgggcccag gcgggggcct
      241 cctcccccag ggcccaggcc gccagggcct cgaggcggcc cacgtacagg tacccccgcc
      301 ggttagggcg ggccaccccg gcgaagggcg aaaggaggtc caggctctcc cggaccaagg
      361 cggccaggtc ctcgtccccg ggccagaaga ggcggtaggc ctccgtggac ttgtcgctgg
      421 tgcaggaaag gggggcctcc cgctccagca cccagggcct gagccccctt cgcgccaaaa
      481 agtaggcggc ggaaaggccg gcaaggcccc cgcccaccac caaagcctcc acgcgccgct
      541 ccatgcccaa agcctccttc cgggttcgcg cccatcgtac accgaggcgg tatcctcttg
      601 ggaagagggg tgccatgctg gaagaagcct tggccgccat ccaaaacgcc cgggacctgg
      661 aggagctcaa agccctcaag gcccgctacc tggggaagaa gggcctcttg acccaggaga
      721 tgaagggcct ttcggccctt cccctggagg agcgccggaa acggggccag gagctgaacg
      781 ccatcaaggc cgccctcgag gcggccctgg aggcgcggga aaaggccctg gaggaggcgg
      841 ccctcaagga ggccttggag cgggagcggg tggacgtgag ccttcccgga gcaagcctct
      901 tctccggggg gctccacccc atcaccctca tggagcggga gctcgtggag atcttccgcg
      961 ccctgggcta ccaggcggtg gagggccccg aggtggagag cgagttcttc aactttgacg
     1021 ccctgaacat ccccgaacac cacccggcgc gggacatgtg ggacaccttc tggctcactg
     1081 gggagggctt taggctggaa ggccccctgg gggaggaggt ggaggggcgc ctcctcctca
     1141 ggacccacac ctcccccatg caggtccggt acatggtggc ccacaccccc cctttccgca
     1201 tcgtggtccc gggccgggtc ttccgctttg agcagacgga cgccacccac gaggccgtct
     1261 tccaccagct ggagggcctg gtggtgggcg aggggatcgc catggcccac ctcaaggggg
     1321 cgatttacga gctcgcccag gccctcttcg gccccgactc caaggtgcgc ttccagcccg
     1381 tctacttccc cttcgtggag cccggggccc agttcgccgt gtggtggccc gaggggggga
     1441 agtggctgga gctgggcggg gccgggatgg tccaccccaa ggtcttccag gcggtggacg
     1501 cctaccggga aaggctcggc ctgcccccgg cctaccgggg ggtcacgggc ttcgccttcg
     1561 ggctcggggt ggagcgcctc gccatgctcc gctacggcat ccccgacatc cgctacttct
     1621 tcgggggcag gctcaagttc ctggagcagt tcaagggggt tctatgaggg tgcccttctc
     1681 ctggctaaaa gcctacgtgc ccgagctgga aagccccgag gtcctggagg agcgcctggc
     1741 gggcctgggg tttgaaacgg accggataga gcgggtcttc cccatcccaa gaggggtggt
     1801 cttcgcccgg gtcctggagg cccaccccat ccccggcacc cggcttaagc gcctggtcct
     1861 ggacgcgggc cggacggtgg aagtggtctc gggggcggaa aacgcccgaa aaggaatcgg
     1921 ggtggccctg gccctccccg ggacggagct tcccggcctg ggccaaaagg tgggggaacg
     1981 ggtcatccaa ggggtgcggt ccttcggcat ggccctctct ccccgggagc tcggggtagg
     2041 ggagtacggc ggggggcttc tggagttccc cgaggacgcc ctcccccccg gcacccccct
     2101 ttcggaggcc tggccggagg aggtggtgct ggacctcgag gtcaccccga accgcccgga
     2161 cgccctgggc cttttgggcc tcgcccggga cctccacgcc ctgggctacg ccctggtgga
     2221 gcccgaagcg gccctgaagg cggaggccct tcccctcccc ttcgccctca aggtggagga
     2281 cccggagggc gccccccact tcaccctggg ctacgccttc ggcctaaggg tggccccaag
     2341 ccccctctgg atgcagcggg ccctcttcgc cgcgggcatg cggcccatca acaacgtcgt
     2401 ggacgtgacc aactacgtca tgctggaaag ggcccagccc atgcacgcct ttgacctgcg
     2461 cttcgtagga gaggggatcg cggtgcgccg ggcgcgggaa ggggagcggc ttaagaccct
     2521 ggacggggtg gaaagaaccc tccaccccga ggacctggtg atcgccgggt ggcgggggga
     2581 ggagagcttc cccttgggcc tcgccggggt catgggcggg gcggagagcg aggtccggga
     2641 ggacacggag gccatcgcct tggaggtggc ctgctttgac ccggtctcca tccgcaagac
     2701 cgcccgccgc cacggcctgc gcaccgaggc gagccaccgc tttgagcggg gggtggaccc
     2761 cctgggccag gtccccgccc agaggcgggc cttaagcctc ctccaggccc tggcgggggc
     2821 ccgggtggcc gaggccctcc tcgaggcggg aagccccaag cccccggagg ccatcccctt
     2881 ccgccccgag tacgccaacc gcctcctggg cacgtcctac cccgaggccg agcagatcgc
     2941 catcctcaag cggctcgggt gccgggtgga aggcgaaggc cccacctacc gggtcacccc
     3001 gcccagccac cgcctggacc tcaggctgga ggaggacctg gtggaggagg tggcccgcat
     3061 ccagggctac gagaccatcc ccttggccct ccccgccttc ttccccgccc ccgacaaccg
     3121 cggggtagaa gccccctacc ggaaggagca gcgcctaagg gaagtcctct ccgggctcgg
     3181 cttccaggag gtctacacct acagcttcat ggaccccgag gacgcccgcc gcttccgcct
     3241 tgatcccccc cgcctcctcc tcctcaaccc cctggccccg gaaaaggcgg ccctcaggac
     3301 ccacctcttc cccggcctgg tccgggtgct gaaggagaac ctggacctgg accggcccga
     3361 gcgggccctc ctctttgagg tgggccgggt cttccgggag cgggaggaga cccacctggc
     3421 cggcctcctc ttcggcgagg gggtgggcct cccctgggcc aaggagcggc tttccggcta
     3481 cttcctcctc aaggggtacc tcgaggccct cttcgcccgg ctcggcctgg ccttccgggt
     3541 ggaggcccag gccttcccct tcctccaccc cggggtctcg ggacgggtcc tggtggaggg
     3601 ggaggaggtg ggcttcctcg gcgccctcca ccccgagatc gcccaggagc tggagcttcc
     3661 ccccgtccac ctctttgagc tccgcctgcc cctcccggac aaacccctcg ccttccaaga
     3721 cccctcccgc caccccgccg ccttccgcga cctggccgtg gtggtccccg cccccacccc
     3781 ctacggcgag gtggaggctt tggtgcggga ggccgcaggc ccctacctgg aaagcctcgc
     3841 cctctttgac ctctaccaag gcccgcccct gcccgaaggc cacaagagcc tcgccttcca
     3901 cctccgcttc cgccacccca agcgcaccct gcgggacgag gaggtggagg aggccgtctc
     3961 ccgggtggcg gaggccctca gggccagggg gttcggcctg cgcggcctgg acaccccgta
     4021 atgccacccc atgctggctt gcgccacgat gggggccccg gcaaaaggcc ctcagagggc
     4081 ttttccggat tagccgggcg aaggaaccgg ggagaaaagg tataagctgg gggcatgctc
     4141 acctgggtgg acctcctggc cctcatggtt ctggccctct ccctggccct ggggtaccgg
     4201 ggggggcttg tcctcgcctg ggtcgggctc ctgggccttc ccctctacgc ggccgccctg
     4261 gccctcggcc tccccgcctt ctggaccgcc ctcgccctgg ggctcgtcct gggggccttg
     4321 gccaaaagcc tccccctctt cctctccgag gcggcggaaa ggggcctcgg gcttctcggg
     4381 ggcggcctcc tcggcctctt cctcgcggcg gccatctgga cgggcttccc cagcgagccc
     4441 gccccttccg gggggatcc
//