From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Trypanosoma brucei (clone WaTat1.1) surface glycoprotein (VSG)
Date: 24 Jul 92 10:10:29 GMT
Distribution: bionet
Checksum: 60801     6

LOCUS       TRBSURGLYA   1705 bp ss-mRNA            INV       24-JUL-1992
DEFINITION  Trypanosoma brucei (clone WaTat1.1) surface glycoprotein (VSG)
            mRNA, complete cds.
ACCESSION   M83694
KEYWORDS    surface glycoprotein.
SOURCE      Trypanosoma brucei cDNA to mRNA.
  ORGANISM  Trypanosoma brucei
            Eukaryota; Animalia; Protozoa; Sarcomastigophora; Mastigophora;
            Kinetoplastida; Trypanosomatina; Trypanosomatidae.
REFERENCE   1  (bases 1 to 1705)
  AUTHORS   Barbet,A.F., Davis,W.C. and McGuire,T.C.
  TITLE     Cross-neutralization of two different trypanosome populations
            derived from a single organism
  JOURNAL   Nature 300, 453-456 (1982)
  STANDARD  full automatic
REFERENCE   2  (bases 1 to 1705)
  AUTHORS   Barbet,A.F., Myler,P.J., Williams,R.O. and McGuire,T.C.
  TITLE     Shared surface epitopes among trypanosomes of the same serodeme
            expressing different variable surface glycoprotein genes
  JOURNAL   Mol. Biochem. Parasitol. 32, 191-200 (1989)
  STANDARD  full staff_review
REFERENCE   3  (bases 1 to 1705)
  AUTHORS   Kamper,S.M. and Barbet,A.F.
  TITLE     Surface epitope variation via mosaic gene formation is potential
            key to long-term survival of Trypanosoma brucei.
  JOURNAL   Mol. Biochem. Parasitol. 53, 33-44 (1992)
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     sig_peptide     86..169
                     /standard_name="VSG"
                     /product="surface glycoprotein"
                     /gene="VSG"
                     /evidence=EXPERIMENTAL
                     /codon_start=1
                     /citation=[2]
                     /citation=[3]
     CDS             86..1624
                     /standard_name="VSG"
                     /product="surface glycoprotein"
                     /gene="VSG"
                     /evidence=EXPERIMENTAL
                     /codon_start=1
                     /translation="MTKSPTKLSQVALSYLLLIAAAPSHVQPASQSALDKGLWQGICK
                     ATAELNKVPGTMTHELTQILQKAKTMRRGELKAQIFGLRHAGTPSANKAAVMAAYFSR
                     RYKQIIEALETQDIEKAIDAAAKAAYLQGRIDDPLHLLAAVDNTNNLCLSTTGATGNK
                     PAKTPGKLGDVDCPLSAPSAKNKAAELSGITQAGFTMLKKDVSSGDAKQVASGTKKCN
                     LLSSVNGQGFGQTEAAISTHHEILGGYITIKDTNTEIDVTAHNKAHTVESGKHESWTA
                     AHAAIMVSPKHTTTPYINETGEIHSRSDMTKVLQAVYSAKPPISNTGLENKVIEIFQG
                     KEAKKLQDYEDAVNQVTIPAGIAGLETDQTLAAVNDEDKLIAIRAFYEQQVGAAYGKM
                     VATISEASNKQTATAPADCGKKLKKVDCKESDGCKWNSTDKSEGEFCKPKGEDEQKAQ
                     GAGTGDGAAGEQKKEDKCTGKKKDDCKDGCKWEGKECKDSSILATKKFALSVVSAAFV
                     ALLF"
                     /citation=[2]
                     /citation=[1]
                     /citation=[3]
     mat_peptide     170..1552
                     /standard_name="VSG"
                     /product="surface glycoprotein"
                     /gene="VSG"
                     /evidence=EXPERIMENTAL
                     /codon_start=1
                     /citation=[2]
                     /citation=[3]
BASE COUNT      623 a    387 c    404 g    291 t
ORIGIN
        1 aacgctatta ttagaacagt ttctgtacta tattgaagag aaaaaaggag tcaagaaaca
       61 tagaaaagct agccatcaaa agaagatgac aaaaagtcca acgaaactga gccaagtagc
      121 gctttcatac ttgttactaa tcgccgcagc accgagtcat gtacaacccg ctagtcaatc
      181 agccttggac aaagggctct ggcagggcat atgcaaagcg acggcagagc tgaacaaagt
      241 tcccgggacg atgacgcacg aattgacgca gatcctccaa aaagcgaaga cgatgcggcg
      301 tggagaactg aaagcacaaa tatttgggct aaggcatgca ggaacaccat cggcaaacaa
      361 agcagcggta atggcggcgt atttcagccg taggtacaag cagataattg aagcgcttga
      421 gacgcaagac atcgaaaaag ccatcgacgc ggcagccaaa gcagcgtacc ttcaaggcag
      481 aatagacgac ccgcttcatt tactggcagc tgtggataac acaaacaatc tttgtctgtc
      541 tacgaccgga gcgacaggca acaaaccggc aaagacgccc ggcaaactgg gcgacgtcga
      601 ctgcccacta tcagcgccgt cagcaaaaaa caaggcagct gaattaagcg gtataacaca
      661 agccggcttt acgatgctaa aaaaggatgt gtcaagcggc gacgccaaac aagtcgcgag
      721 tggcacaaag aaatgtaacc ttctaagttc agtaaacggc caaggcttcg gacagacaga
      781 ggcggcgatc agcacccacc acgaaatact cggaggatac attacaatca aagacacaaa
      841 cactgaaata gacgtaaccg cgcacaacaa agcacacaca gtcgaaagcg gcaagcacga
      901 aagctggacg gcggcacatg cagccattat ggtgagccca aaacacacaa cgacacccta
      961 tatcaatgaa actggggaaa ttcacagcag gtcagacatg acaaaagtgt tgcaagccgt
     1021 ttacagcgca aaaccgccga taagcaacac tggcctagag aacaaggtca tagaaatatt
     1081 tcaaggaaaa gaagcaaaga aactgcaaga ctacgaagac gctgtaaacc aagtaacgat
     1141 accagccggc atagcaggac tggaaactga ccagacgcta gcagcagtaa acgacgaaga
     1201 caaactaatc gcaatcagag ccttttatga acagcaggtc ggcgcagcct atggcaaaat
     1261 ggtagcgacg atatccgaag caagcaataa acaaacagcc accgccccag cagactgcgg
     1321 taaaaaacta aagaaagtag actgcaaaga gagcgatgga tgcaagtgga atagcactga
     1381 caaaagtgaa ggagaattct gcaaacctaa aggggaagat gaacaaaaag cccaaggagc
     1441 aggaacagga gatggagctg caggagaaca aaagaaagag gataaatgca cagggaagaa
     1501 aaaggatgac tgtaaagatg gatgcaaatg ggaaggaaaa gaatgcaaag attcctctat
     1561 tctagcaact aaaaaattcg ccctcagcgt ggtttctgct gcctttgtgg ccttgctttt
     1621 ttaaaacaaa ttttttccct ctctttttaa aattttcctt tgctaaaatt cttgctactt
     1681 gaaaaacttt ctgatatatt ttaac
//