HDTenEightyP/NetNews huggingface dataset:
From: pww@bnr.ca (Peter Whittaker)
Subject: Re: Was Lousy Service
Organization: Bell-Northern Research Ltd., Ottawa
Date: Fri, 2 Oct 1992 20:07:15 GMT

In article <1992Oct2.195746.1563@bcars6a8.bnr.ca> michael.hahn@bnr.ca writes:
>I could be wrong, maybe this wasn't really good service. Maybe I only
>thought it was? Maybe I expect to much? I don't think so.

You expect the right thing.  There are far too few companies and businesses
that know what "service" means.

My favourite local business is Dewar's Audio:  the customer service
they offer is the only reason I bought there, and not elsewhere.  After
dealing with them, I will not even consider buying at a place that
offers less.

pww
From: inunn@hpb.hwc.ca
Newsgroups: ott.general
Subject: Text of Contitutional Accord
Date: 28 Sep 92 11:04:32 EDT
Organization: Health and Welfare, Canada

I believe that hwc.info news group ha the text of the accord.
Newsgroups: bnr.general,ott.general
From: javery@bnr.ca (Joel Avery)
Subject: Re: Was Lousy Service
Reply-To: javery@bnr.ca
Organization: Bell-Northern Research
Date: Fri, 2 Oct 1992 20:28:30 GMT

In article <1992Oct2.195746.1563@bcars6a8.bnr.ca>, mikhahn@bnr.ca (Michael Hahn) writes:
|> 
|> Just in case anyone is interested in the latest w.r.t the lousy
|> service I reported from one of our local computer shops.
|> 
|> I got a call from another one of the owners and can you guess what
|> his response was to my posting.
|> 
|> C) You are wrong about being treated badly by our service department. 
|>    You can not go around posting negative things about our company.
|>    I have sued people before and have never lost. In one case the quy
|>    lost his house defending the lawsuit. So if you don't post a retraction
|>    saying that you were mistaken and angry, I will sue you to defend my
|>    companies reputation and it will cost you thousands of dollars to defend
|>    yourself.
|> 
Hmmmm.  I didn't think that was how things worked.  I thought you could post
your opinion on things, as long as it is in a forum where the other person
could respond to your claims.  And, since the Internet is accessible to this
store (as they saw your post), could they not respond?  

Now, what you can't do, is print flyers, and walk down the street handing them 
out, as the store could not tell the people who took your flyers their side of
the story.

Anyone with a <correct> legal opinion?

-- 

Joel Avery   <javery@bnr.ca>
From: heyendal@Software.Mitel.COM (Carl Heyendal)
Newsgroups: ott.general
Subject: stock broker recommendations
Date: 6 Oct 92 12:54:24 GMT
Organization: Mitel. Kanata (Ontario). Canada.

Can anybody out there share with me your opinion/experiences that you might
have with a stock brokerage firm called A.C. McPherson. 

This firm is encouraging me to do business with them and I would like
to know if they can be considered reputable.

thanx in advance

Carl H.


Newsgroups: ott.general
From: khor@bnr.ca (Kyle Hor)
Subject: RE: LOW gas prices in West End!
Reply-To: khor@bnr.ca (Kyle Hor)
Organization: Bell-Northern Research Ltd.
Date: Mon, 28 Sep 92 13:49:03 GMT

In article <199292736226@gunshy.ocunix.on.ca>, ken@gunshy.ocunix.on.ca (Ken Walker) writes:
|> Most of the time the only thing I top up at gas stations is the
|> air in my bike tires...., you people probably spend more energy
|> driving around to find cheap prices than simply filling up at a
|> location ON your daily route. Of course, I could be full of
|> hot air (or gas).
|> 
Yes and no!  I fill up on my regular route home because it happens to
be among the cheapest in the west end.  It's at MacEwen along Baseline
(& Pinecrest) and selling at 55.9cents/litre.  But if I get to know of
a gas war in the west end I'll spend my little precious time going
there. 
 
If the majority just fill up at their convenience, then there will be
no incentive for the stations to lower their prices.  It's no wonder
Ottawa gas prices are so high.

Cheapskate as it may sound, but I'll continue to do my part till
prices are comparable to Toronto's.  
-- 
...Kyle             khor@bnr.ca         (613) 763-2488
Newsgroups: ott.general
From: cantin@nrccsb3.di.nrc.ca (Claude Cantin)
Subject: Re: October 26th Referendum
Organization: National Research Council of Canada
Date: Fri, 2 Oct 92 20:35:56 GMT

Can't we keep this discussion in the "politics" news groups????

"ott.general" was not formed to discuss political matters...

      Claude
Newsgroups: tn.talk
From: kovarik@martha.utcc.utk.edu (StMeechTheThwacked)
Subject: Awesome Pnews
Organization: University of Tennessee Computing Center
Distribution: tn
Date: Tue, 6 Oct 1992 19:59:40 GMT


In my capacity as a User Services dude, I hereby test this
Pnews on Martha and possibly later on any Sun that I can
think of, and declare that it is the most awesome Pnews
I have ever seen.

				Mitch
Newsgroups: tn.talk
From: kovarik@UTKUX1.UTK.EDU (StMeechTheThwacked)
Subject: Even More Impressed!!!!
Organization: University of Tennessee Computing Center
Distribution: tn
Date: Tue, 6 Oct 1992 20:18:13 GMT

I'm defintely even more impressed now!  Much more so!
					M
From: CZJ@CU.NIH.GOV
Newsgroups: bionet.software
Subject: Re:  Genbank FASTA server -> NCBI?
Date: 1 Oct 92 13:21:20 GMT
Distribution: bionet


Regarding the difficulties of putting up both FASTA and BLAST,
and the e-mail traffic on the subject, has anyone done a
comparison of the results obtained from both?

I suspect some one has and it would be interesting to post the
results and/or the references.

Jim Cassatt
From: zxmkr08@mailserv.zdv.uni-tuebingen.de (Cornelius Krasel)
Subject: Re: Sequence film scanning systems?
Organization: Comp. Center (ZDV) U of Tuebingen, FRG
Date: Wed, 30 Sep 1992 16:26:46 GMT

In <zxmkr08.717690429@mailserv> I wrote:

/* asking for a sequence gel scanning system */

Some people have mentioned the NCSA GelReader program for
Macs, so I ftp'd it today and we had a look. It seems to
be quite nice, but what we are really dreaming of is a program
which reads your TIFF (or whatever) file, you mark the lanes and
it outputs the sequence.

Is there a program like this (either for Mac or PC)? BTW, I read
in the MacVector manual today about the IBI Gel Reader. Is such
a system considered better than an automagical system as described
above?

Quite confused, Cornelius.

PS: I would still be glad if you'd reply by email to
    hashemol@mpib-tuebingen.mpg.dbp.de. My colleague
    was quite impressed :-)
>our lab is pondering about buying a scanner to evaluate DNA
>sequence films. Before spending huge amounts of money :-)
>I would be very happy if you would share your experiences
>with some of my colleagues who are very sceptical about
>BioNet. Thus I would be glad if you'd response by
>email (since those colleagues don't have a Usenet access)
>to hashemol@mpib-tuebingen.mpg.dbp.de.

>In particular we would be interested if we should buy
>a specialized system (such as advertised by IntelliGenetics
>in a recent Nature issue) or if things could be worked
>out with a scanner and some additional software.

>Software may be Mac- or PC-based.

>Thanks in advance, Cornelius.
>--
>/* Cornelius Krasel, Department of Physiological Chemistry, U Tuebingen    */ 
>/* email: krasel@mailserv.zdv.uni-tuebingen.de (Internet)                  */
>/*        krasel@chemie.uni-tuebingen.dbp.de (WIN/X400)                    */
>/* "People are DNA's way of making more DNA." (anonymous)                  */
--
/* Cornelius Krasel, Department of Physiological Chemistry, U Tuebingen    */ 
/* email: krasel@mailserv.zdv.uni-tuebingen.de (Internet)                  */
/*        krasel@chemie.uni-tuebingen.dbp.de (WIN/X400)                    */
/* "People are DNA's way of making more DNA." (anonymous)                  */
From: deboer@bio.vu.nl (Thon de Boer)
Subject: Re: Genbank FASTA server -> NCBI?
Organization: VU Biology, Amsterdam, The Netherlands
Distribution: bionet
Date: Sat, 26 Sep 1992 07:56:29 GMT

kristoff@genbank.bio.net (David Kristofferson) writes:
: mathog@seqvax.caltech.edu (David Mathog) writes:
: 
: >Is the NCBI going to continue the Genbank Fasta e-mail server?
: 
: >If yes, could someone please post the details?
: 
: >If no, why not?
: 
: I was told so time ago by people at NCBI that there are no plans to do
: so, but also that this might be reconsidered.
: 
: I will add myself that FASTA and BLAST use different database formats,
: and this consumes lots of disk space.  As the databases continue to
: grow it would be nice to consolidate formats for searching programs.
: 
: Don't forget that EMBL provides a FASTA server at the address
: fasta@embl-heidelberg.de.  The added load of everyone migrating there
: from genbank.bio.net on top of their current users might be a bit much
: however.  I'll leave further comments to the folks from EMBL and NCBI.
  
There is another way of doing FASTA searches. The FLAT server in Japan
also provides FASTA searches using email. This is featured in my UNIX program
mailfasta3.0 which can be found at the iubio archive and our own FTP archive
bio.vu.nl:/molbio. I also put up an gopher hole (grampus.bio.vu.nl port 9001)
via which mailfasta can be reached.

You can get help from the FASTA server in Japan by sending help in an email
to flat-netserv@smlab.eg.gunma-u.ac.jp

Hope this helps,

Thon de Boer
From: Rob.Harper@csc.fi (Rob Harper)
Newsgroups: bionet.software
Subject: Gopher for Windows 3.1 (ZGopher 0.0.16)
Date: 29 Sep 92 12:39:08 GMT
Distribution: bionet
Original-To: bio-soft@uk.ac.daresbury


MS Windows Gopher reader for Windows 3.1...


- on "ftp.cica.indiana.edu" in pub/pc/win3/uploads, "zgopher0.exe"...
- written in MS Visual Basic
- Alpha release 
- feel free to tune the available sources

-- 
|	  Christian Zufferey
|	  PC Support, Central Computing Service
|	  Swiss Federal Institute of Technology
|	  EPFL, Ecublens, CH-1015 Lausanne
|                                        ___   ___   _   ___     | |
|    Tel: +41-21.693.22.11              /__   /_    /    /     |     |
|    Fax: +41-21.693.22.20             ___/  /     /    /        | |
| E-mail: zufferey@sic.epfl.ch
From: rybicki@uctvax.uct.ac.za
Newsgroups: bionet.software
Subject: Re: plasmid maps from rest. frag. sizes?
Date: 30 Sep 92 16:43:20 +0200
Distribution: bionet
Organization: University of Cape Town

In article <BRIANF.92Sep21172418@dna.uvm.edu>, brianf@dna.uvm.edu (Brain Foley) writes:
> 	I am looking for a program (Mac, DOS, or VMS) that will help
> me draw a circular (or linear) map of DNA, given a list of the
> restriction fragment sizes that are observed upon single and
> multiple-enzyme digests.
> 	I have looked in the IBMPC and MAC subdirectories of the
> MOLBIO directory of FLY.BIO.INDIANA.EDU FTP site.  I did not see what
> I wanted there, but I may have missed something.

I must heartily recommend a package called RESOLVE for PC, available from Prof.
Eric Harley, UCT Medical School, PO Observatory 7925, South Africa (or, more
obviously for this forum, harley@chempath.uct.ac.za or @uctvax.uct.ac.za). 
This assists in mapping in that it tells you when the (or a) map is obtainable
from the data; it also tells you how MANY maps can be made from the same data
given different errors in measurement of band size!  It can be used as a map
database, and even to compare any number of maps in terms of phylogenetically
informative sites, or proportion of sites shared.  Costs US$100 from him
direct (I have no interest, I just like the package).
 _________________________________________________________________________
|                                 |                                       |
|  Ed Rybicki                     |    Now you've got the hang of it      |
|  Dept Microbiology              | There's nothing you can't do with it  |
|  University of Cape Town        |         If you're very into it        |
|  PB Rondebosch 7700             |         You can't go wrong....        |
|  South Africa                   |                                       |
|  fax 27 21 650 4023             |              - Mad John               |
|  ed@micro.uct.ac.za             | (Ogden's Nut-Gone Flake, Small Faces) |
|_________________________________|_______________________________________|
From: Fergus_Doherty@vme.ccc.nottingham.ac.uk
Newsgroups: bionet.software
Subject: Mac-based analysis of electron micrographs?
Date: 1 Oct 92 09:39:22 GMT
Distribution: bionet
Original-To: BIO-SOFT@uk.ac.daresbury

Hello, does anyone use a Mac to do quantitative morphometric analysis 
of electron micrographs on Mac?  If so what software/hardware do you 
use?  I know of NIH Image, but I was wondering about a more specialist 
probably commercial package.  Would also need to do counting of 
imunogold particles.  Thanks for any advice.
P.S. the varied nature of some of my postings is due to requests I get 
from colleagues for this kind of info!
Fergus Doherty, Nottingham University, UK.
From: mrl775@tigger.jvnc.net
Subject: Re: GCG7 to Paup translation? (A)
Followup-To: bionet.software
Reply-To: mrl775@tigger.jvnc.net
Organization: TheNews 
Date: Tue, 6 Oct 1992 13:41:47 GMT

In article <16237519@um.cc.umich.edu> Doug.Eernisse@UM.CC.UMICH.EDU writes:
>Jeremy John Ahouse asks:
>>I need to find a program that will take me from the multiple sequence
>> alignments that I can get out of GCG 7's pileup program to the format
>>-required
>> by Paup on the Mac.
>>
>>         Do you all do this by hand  Do you have a good/better multiple
>> sequence alignment tool on the Mac?
> 
>My HyperCard 2.x stack, DNA Translator, does that conversion. Be
>sure you get the most recent version (v. 1.0k6) because the last
>version (1.0k5) was one of the first to support Pileup and there 
>was one line of code out of place introduced when I also added support
>for input/output formats for Jotun Hein's (Unix) Treealign program. 
>Other multiple sequence alignment formats that can be converted to 
>either Paup (Nexus) or simple named string format are simple 
>"interleave" format, Eugene (or Prophet), ClustalV, and Phylip 
>"outfile" format.

My version of PAUP for the Mac, version 3.0s, includes the ability to import
data from gcg msf files.  Perhaps you should pick up the latest version of PAUP
from the University of Illinois.

The information is:

David L. Swofford
Illinois Natural History Survey
607 E. Peabody Dr.
Champaign, IL  61820

The program is $50.

Keith

ps.  the program lists DAVESWOF@UIUCVMD.bitnet as Daves address.
From: pgil@TEMIN.LANL.GOV (Paul Gilna)
Newsgroups: bionet.software
Subject: Re: NCBI FASTA mail server
Date: 5 Oct 92 19:24:53 GMT
Distribution: bionet


on question 1:

> 1. Is it still possible to submit sequences by E-mail using AUTHORIN?


	Los Alamos National Laboratory has entered into an interagency
	agreement with the NCBI and will continue to handle all direct
	submissions of data by email and on floppy disc, using the
	Genbank submission form, Authorin and on-line access. LANL will
	also provide updating services for submitted data. There is
	therefore little change to the submissions process as it existed
	under the previous Genbank contract.

	for more information on electronic data submission to GenBank,
	mail a message containing as text the word "help" to:

			bioserve@genome.lanl.gov

			or call (505) 665 2177



--paul


Paul Gilna
co- Principal Investigator
Genbank at LANL
From: VANNESTEJ%RUA1@INVERMAY.CRI.NZ
Newsgroups: bionet.software
Subject: RE:Plasmid Drawing Program
Date: 1 Oct 92 08:37:53 GMT
Distribution: bionet

In response to " Does anyone out there know of a simple Macintosh program that 
will produce a drawing of a plasmid identifying restriction enzyme sites
by enzyme name and location using just a list of restriction enzymes
with numbers (digestion sites)?  I don't need the accuracy a GCG
program, just something simple and quick"

	Couple years ago a program called Plasmid draw, developed by Wolfram Siede, 
was published in BioTechniques vol 5 n 5.  The program has been improved by 
comparison to what has been published .  It allows to draw the plasmid as a 
circular or as a linear map,on which you can add some genes. 

The address of Wolfram Siede was
Dept of Pathology, R 208
Stanford University
School of medicine
Stanford, CA 94305

	Hope this help,

	Joel

	Joel L. Vanneste
	The Horticulture and Food Research Institute of New Zealand Ltd 
	Ruakura Agricultural Centre,		
	Private Bag 3123, Hamilton,		e mail: VANNESTEJ%RUA1@INVERMAY.CRI.NZ  
	New Zealand						fax 64 7 838 5073
From: MANGALAM@SALK-SC2.SDSC.EDU (Harry J Mangalam)
Newsgroups: bionet.software
Subject: PCPRIMER - An Apology
Date: 5 Oct 92 21:27:38 GMT
Distribution: bionet

Hi ,
My abject apologies to those of you inconvenienced by my daftness.
The primer zip  archive has been set with the right protection so that 
the pubklic can ftp it.

Sorry,
Harry
(almost on vacation)


tarion
Ignore anything below this line
 exo
From: ahouse@hydra.rose.brandeis.edu
Newsgroups: bionet.software
Subject: Problems with Gopher - Help
Date: 3 Oct 92 21:01:24 GMT
Organization: Brandeis University


	I have a POP 3 server on the Unix machine that I connect to.  I can
use Eudora.  I can send messages from inside SeqApp.  But, when I try connect
to Gopher sites I just hang forever waiting for the connect.

	Any suggestions????

	- jeremy

:::::::::::::
	Jeremy John Ahouse
	Center for Complex Systems and Biology Dept
	Brandeis University
	Waltham, MA 02254-9110

	(617) 736-4954
	email: ahouse@hydra.rose.brandeis.edu
From: francis@monod.Biol.McGill.CA (Francis Ouellette)
Newsgroups: bionet.software
Subject: Re: Sequence film scanning systems?
Date: 3 Oct 92 18:41:06 GMT
Organization: McGill University

zxmkr08@mailserv.zdv.uni-tuebingen.de (Cornelius Krasel) writes:

>our lab is pondering about buying a scanner to evaluate DNA
>sequence films. Before spending huge amounts of money :-)

>In particular we would be interested if we should buy
>a specialized system (such as advertised by IntelliGenetics
>in a recent Nature issue) or if things could be worked
>out with a scanner and some additional software.

I have used the Millipore system, and it is quite good, but be ready
to pay major $$$.  The very big advantage of such a system is similar
to what you find on automated sequencing packages, once you do your
melds, you can display the contigs with the multiple sequences
alligned, and you can then call up all of the images.  It will present
them all in the same orientation (let us say A,C,G and T) independant
of the orientation of the gel.  From looking at these images you can
see why a certain discrepency exist at a certain position.  

What does it cost?  Well, I dont have the list prices here, but you
are probably looking at $60-70,000 for the basic system, which is a
small sparc 2 (eg IPX), a scanner and their software.  I only know
that the software alone (part a=reading the gels, part b= gel project
manager) is about $28,000 (canadian, last january).

Is this worth it? If you do _a lot_ of sequencing, it may be.  But if
you do a lot of sequencing ... maybe you should be looking at
automation ...  

A note:  A single $28,000 software licence will give you _one_ user
that can use this package at any given time, even if this workstation
is networked.  Also, at this time, it only runs under Open Look Window
Manager version 3, no other platform is supported.  

I hope this helps in your descision making,

regards,

francis

--
| B.F. Francis Ouellette  
| manager, yeast chromosome I project
| dept of biology, McGill university, Montreal, Qc, Canada
| francis@monod.biol.mcgill.ca
From: karabinos@exmed1.dnet.gwdg.de ("EXMED1::KARABINOS")
Newsgroups: bionet.software
Subject: sub
Date: 28 Sep 92 08:55:47 GMT
Distribution: bionet
Original-To: bio-soft@uk.ac.daresbury

sub bionet.software
From: Doug.Eernisse@UM.CC.UMICH.EDU
Newsgroups: bionet.software
Subject: GCG7 to Paup translation? (A)
Date: 4 Oct 92 18:21:20 GMT
Distribution: bionet

Jeremy John Ahouse asks:
>I need to find a program that will take me from the multiple sequence
> alignments that I can get out of GCG 7's pileup program to the format
>-required
> by Paup on the Mac.
>
>         Do you all do this by hand  Do you have a good/better multiple
> sequence alignment tool on the Mac?
 
My HyperCard 2.x stack, DNA Translator, does that conversion. Be
sure you get the most recent version (v. 1.0k6) because the last
version (1.0k5) was one of the first to support Pileup and there 
was one line of code out of place introduced when I also added support
for input/output formats for Jotun Hein's (Unix) Treealign program. 
Other multiple sequence alignment formats that can be converted to 
either Paup (Nexus) or simple named string format are simple 
"interleave" format, Eugene (or Prophet), ClustalV, and Phylip 
"outfile" format.
 
The most recent version of my stack is usually on my account:
ftp as "anonymous" (password "guest") to "um.cc.umich.edu"
then "cd gdef" and "get dnastack.hqx"
You will need to debinhex the file, as is usual for Mac file 
transfers, and this will result in a self-extracting archive.
On ftp.bio.indiana.edu you can find the same thing either
in /Incoming/DNAstacks.10k6.hqx or eventually in
/molbio/mac, but I do not upload current versions there as
frequently.
 
This also includes a manual sequence aligner stack called Aligner.
Perhaps the most useful features of Aligner are the various
nucleotide or amino acid coloring routines. These are not especially
fast but have options that I haven't seen in other packages.
For example, you can color nucleotides in triplets according to
their inferred amino acid coding. This, combined with the
ability to match the sequences with dashes where they are identical
to the first sequence, gives an informative view of a protein-
encoding DNA sequence alignment. You can also color according
to hydrophobicity, chemical, function, etc. groupings of the
amino acids. Be sure to increase the memory partition of HyperCard
to a minimum of 3 MB if you want to do much of this.
The input format for your sequences is the "named string" format
resulting from the above-mentioned conversions:
 
name_1<space(s)>ACTG...<cr>
name_2<space(s)>ACCG...<cr>
etc.
 
A descriptions of these stacks was published recently in CABIOS
8:177-184, but that was before the above-mentioned features were
added.
 
Doug Eernisse
Doug_Ee@um.cc.umich.edu
Museum of Zoology, Univ.of Michigan
Ann Arbor, MI 48109 USA
 
Almost forgot to mention: The stacks and many of the custom
external commands/functions employed come with "free for
noncommercial use only" restrictions.