From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Bacillus subtilis menaquinone operon.
Date: 28 Jan 93 05:45:50 GMT
Distribution: bionet
Checksum: 49480 19

LOCUS       BACMENAQOP   5932 bp ds-DNA             BCT       27-JAN-1993
DEFINITION  Bacillus subtilis menaquinone operon.
ACCESSION   M74538 M74182 M74183
KEYWORDS    menaquinone.
SOURCE      Bacillus subtilis (strain RB1) DNA.
  ORGANISM  Bacillus subtilis
            Prokaryotae; Firmicutes; Endospore-forming rods and cocci;
            Bacillaceae.
REFERENCE   1  (bases 1 to 5932)
  AUTHORS   Miller,P.F., Mueller,J., Hill,K. and Taber,H.W.
  TITLE     Transcriptional regulation of a promoter in the men gene cluster of
            Bacillus subtilis
  JOURNAL   J. Bacteriol. 170, 2742-2748 (1988)
  STANDARD  full automatic
REFERENCE   2  (sites)
  AUTHORS   Tuerk,C., Gauss,P., Thermes,C., Groebe,D.R., Gayle,M., Guild,N.,
            Stormo,G., d'Aubenton-Carafa,Y., Uhlenbeck,O.C. and Tinoco,I.Jr.
  TITLE     CUUCGG hairpins: Extraordinarily stable RNA secondary structures
            associated with various biochemical processes.
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 85, 1364-1368 (1988)
  STANDARD  full automatic
REFERENCE   3  (bases 1 to 5932)
  AUTHORS   Driscoll,J.R. and Taber,H.W.
  TITLE     Sequnce organization and regulation of the Bacillus subtilis menBE
            operon
  JOURNAL   J. Bacteriol. 174, 5063-5071 (1992)
  STANDARD  full automatic
REFERENCE   4  (bases 1 to 5932)
  AUTHORS   Rowland,B., Hill,K., Mueller,J., Driscoll,J.R. and Taber,H.W.
  TITLE     Organization of an operon involved in menaquinone biosynthesis in
            Bacillus subtilis.
  JOURNAL   Unpublished (1993)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     -35_signal      13..18
                     /evidence="EXPERIMENTAL"
                     /citation=[4]
                     /citation=[1]
     misc_signal     13..27
                     /function="regulatory site for pH regulation of promoter"
                     /citation=[4]
                     /citation=[1]
                     /note="putative"
     -10_signal      35..40
                     /evidence="EXPERIMENTAL"
                     /citation=[4]
                     /citation=[1]
     mRNA            47..>579
                     /evidence="EXPERIMENTAL"
                     /citation=[4]
                     /citation=[1]
                     /note="5' RNA terminus mapped by high-resolution S1
                     nuclease protection assays [J. Bacteriol. 170, 2742-2748
                     (1988)]; exact 3' terminus unclear, but transcripts into
                     menE are undetectable; contains ORF1 to possible ORF5"
     RBS             128..133
                     /citation=[4]
                     /citation=[1]
                     /note="ORF2 is apparently linked translationally to ORF1
                     (entC); ORF1 and ORF2; putative"
     misc_signal     4089..4103
                     /function="involved in pH regulation of promoter"
                     /phenotype="unknown"
                     /citation=[3]
                     /note="sequence motiff seen in promoter regions for
                     several B.subtilis genes involved in TCA cycle and
                     electron transport; putative"
     -35_signal      4102..4107
                     /gene="menB"
                     /evidence="EXPERIMENTAL"
                     /citation=[3]
                     /note="identified by appropriate spacing from mapped 5'
                     menB RNA and homology to B. subtilis vegetative type
                     promoters"
     -10_signal      4125..4130
                     /gene="menB"
                     /evidence="EXPERIMENTAL"
                     /citation=[3]
                     /note="identified by correct spacing from a mapped 5' end
                     of menB transcripts"
     mRNA            4138..>5722
                     /gene="menB"
                     /evidence="EXPERIMENTAL"
                     /citation=[3]
                     /note="5' RNA terminus mapped by high resolution primer
                     extension assays.; Exact 3' terminus is unclear but
                     extends at least through menE"
     RBS             4148..4152
                     /gene="menB"
                     /citation=[3]
                     /note="putative"
     terminator      4963..4993
                     /gene="menB"
                     /evidence="EXPERIMENTAL"
                     /citation=[3]
                     /note="contains a loop sequence shown to provide unusuall
                     stability (see misc. feature 4975-4982)"
     misc_feature    4975..4982
                     /function="possible stabilization of RNA secondary
                     structure"
                     /standard_name="'Golden Loop' sequence"
                     /gene="menB"
                     /evidence="EXPERIMENTAL"
                     /citation=[2]
                     /citation=[3]
                     /note="sequence is very similar to that shown by Tuerk et
                     al [Proc. Natl. Acad. Sci. U.S.A. 85, 1364-1368 (1988)] to
                     confer stability to RNA stem-loop structures"
     misc_signal     4986..5013
                     /function="recognition sequence for pH regualtion of
                     promoter"
                     /phenotype="unknown"
                     /gene="menE"
                     /citation=[3]
                     /note="putative"
     -35_signal      5002..5007
                     /gene="menE"
                     /evidence="EXPERIMENTAL"
                     /citation=[3]
     -10_signal      5024..5029
                     /gene="menE"
                     /evidence="EXPERIMENTAL"
                     /citation=[3]
     mRNA            5038..>5932
                     /gene="menE"
                     /evidence="EXPERIMENTAL"
                     /citation=[3]
                     /note="5' RNA terminus mapped by high resolution primer
                     extension assays.; Exact 3' terminus is unclear"
     RBS             5051..5056
                     /gene="menE"
                     /citation=[3]
                     /note="putative"
     CDS             142..579
                     /citation=[4]
                     /note="putative;  operon regulatory gene; ORF1"
                     /codon_start=1
                     /translation="MVTTVQRTFRKKVLHALHKAKEVNHAVLISYSRQIESLDPLSFF
                     NYGAKKYTGNRFFWSDPESELTIVGLGKEAVFQTNQKNSERYREVFEQWERFKKTAFH
                     IYEEEKLQWDLCYSEDFLLTLAKKEVHNGTISRKGISLCLRLC"
     CDS             582..1547
                     /function="unknown"
                     /gene="entC"
                     /citation=[4]
                     /note="ORF2"
                     /product="isochorismate synthase"
                     /codon_start=1
                     /translation="MTAEGPFLTVNRWVSGGEDAEAVLEGLKAFAAEFMVPDFKQEDQ
                     AVIAAAEELDKDDWLKAIETATSQIKEKQYDKVVLARELLLTFDGPIQIEPVLKTLLD
                     DQQTSYVFAIEQEGKTFVGASPERLIKRDGGTVMSSCLAGSIKRGVNEEDDRRIGLEL
                     LNDEKNLLEHDIVVGMIHNAFVSSCSEVEKPDGPVLYKTKSVQHLFTPIVGQLRESAS
                     IFDLIEKLHPTPALGGSPQEKAVDVIREIEPMSRGWYAAPIGWIDSQDNGEFAVAIRS
                     GLIEGSTARLFAGCGIVEDSEPISEYEETQIKLKPMISALGGERR"
     CDS             1624..2049
                     /function="convert isochorismate to SHCHC"
                     /standard_name="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /gene="menD"
                     /citation=[4]
                     /codon_start=1
                     /translation="MSGLKIYTLAVLCAAHPDISVHVQIDERSAGFFALGLAKAKQRP
                     VLLICTSGTAAANFYPAVVEAHYSRVPIIVLTADRPHELRRVGAPQAINQHFLFGNFV
                     KFFTDSALPEESPQMLRYIRTLASRAAGEAQKRPMALSM"
     CDS             2072..3187
                     /gene="menCF"
                     /evidence="EXPERIMENTAL"
                     /citation=[4]
                     /note="ORF's for menC and menF are apparently fused into
                     one open reading frame"
                     /codon_start=1
                     /translation="MPDLSDEPFGRMRTGRHVSVKTGTQSVDRESLSDVAEMLAEAEK
                     GMIVCGELHSDADKENIIALSKALQYPILADPLSNLRNGVHDKSTVIDAYDSFLKDDE
                     LKRKLRPDVVIRFGPMPVSKPVFLWLKDDPTIQQIVIDEDGGWRDPTQASAHMIHCNA
                     SVFAEEIMAGLTAATRSSEWLEKWQFVNGRFREHLQTISSEDVSFEGNLYRILQHLVP
                     ENSSLFVGNSMPIRDVDTFFEKQDRPFRIYSNRGANGIDGVVSSAMGVCEGTKAPVTL
                     VIGDLSFYHDLNGLLAAKKLGIPLTVILVNNDGGGIFSFLPQASEKTHFEDLFGTPTG
                     LDFKHAAALYGGTYSCPASWDEFKTAYARRQTSPDSI"
     CDS             3270..3857
                     /function="unknown"
                     /citation=[4]
                     /note="ORF5"
                     /codon_start=1
                     /translation="MGTVNITVSDGVRYAVADEGPNASEAVVCLHGFTGSKQSWTFLD
                     EMLPDSRLIKIDCLGHGETDAPLNGKRYSTTRQVSDLAEIFDQLKLHKVKLIGYSMGG
                     RLAYSFAMTYPSGYRHLCLKARRRGSKHLGNGGNESCGTGSLLILFYETGSKRLLRIG
                     RISLCFHLSRGWLKIFGTGYDRAVCGTIRLGLRTV"
     CDS             4157..4942
                     /function="convert OSB-CoA to DHNA in menaquione
                     biosynthesis"
                     /standard_name="Men B"
                     /gene="menB"
                     /evidence="EXPERIMENTAL"
                     /citation=[3]
                     /note="predicted MW of 28584.4 Daltons, 261 a.a. residues,
                     estimated pI= 5.99"
                     /product="dihydroxynapthoic acid (DHNA) synthetase"
                     /codon_start=1
                     /translation="MKIFDETYNGIAKITINRPEVHNAFTPKTVAEMIDAFADARDDQ
                     NVGVIVLAGAGDKAFCSGGDQKVRGHGGYVGDDQIPRLNVLDLQRLIRVIPKPVVAMV
                     SGYAIGGGHVLHIVCDLTIAADNAIFGQTGPKVGSFDAGYGSGYLARIVGHKKAREIW
                     YLCRQYNAQEALDMGLVNTVVPLEQLEEETIKWCEEMLEKSPTALRFLKAAFNADTDG
                     LAGIQQFAGDATLLYYTTDEAKEGRDSFKEKRKPDFGQFPRFP"
     CDS             5060..5722
                     /function="convert OSB to OSB-CoA in menaquinone
                     biosynthesis."
                     /standard_name="MenE"
                     /gene="menE"
                     /evidence="EXPERIMENTAL"
                     /citation=[3]
                     /note="predicted MW: 24424.31 Daltons, 220 a.a. residues,
                     and a predicted pI=5.92"
                     /product="o-succinylbenzoic acid (OSB) Co-A Ligase"
                     /codon_start=1
                     /translation="MQRAQLTPERIALIYEDQTVTFAELFAASKRMAEQLAAHSVRKG
                     DTAAILLQNRAEMVYAVHACFLLGVKAVLLNTKLSTHERLFQLEDSGSGFLLTDSSFE
                     KKEYEHIVQTIDVDELMKEAAEEIEIEAYMQMDATATLMYTSGTTGKPKGVQQTFGNH
                     YFSACRPLLFGITEQDAGLCIAALHISGLSALFKSVIYGMTVVLHQRFSVTECAAFYQ
                     HS"
BASE COUNT     1594 a   1248 c   1552 g   1538 t
ORIGIN      
        1 aagcttgttt ctttttcatt tctgaaatta ggtttataat aggtaaggca ggccatttgg
       61 actgcatgat ctgtgtttga cacaaaggag acacaggtgt atggtattaa aagcatttta
      121 aggattatgg aggaatgtct catggtgaca acggtgcagc gtacgttccg aaagaaggtt
      181 ctacatgcat tacataaagc caaagaagtc aaccatgctg tcttaataag ctattcgaga
      241 caaatcgagt ctcttgaccc tctatcattt ttcaattacg gagcaaaaaa atatacaggc
      301 aatcgatttt tttggtcaga tcctgaaagt gaattgacaa tagtcggtct tggcaaagaa
      361 gcggttttcc agacaaatca aaaaaacagc gagcggtatc gtgaggtttt tgaacaatgg
      421 gagcgcttta aaaagacggc ttttcatatt tatgaagaag aaaagctgca gtgggacctg
      481 tgttattcgg aggattttct tttgaccctt gcgaagaaag aggttcacaa tgggaccatt
      541 tctcggaagg ggatttcttt gtgcctgcgc ttatgctgac gatgactgct gaaggcccgt
      601 tcttaacagt taacagatgg gtaagcggag gagaagacgc agaagctgtt ttagaaggct
      661 taaaagcttt tgcggcggaa tttatggttc ccgatttcaa gcaagaagat caggctgtga
      721 ttgcagcagc cgaagagctg gataaggatg attggctgaa agcaatcgaa acggccacaa
      781 gccaaattaa agagaaacaa tatgataagg ttgttcttgc ccgagagctg ctgctcacgt
      841 ttgacggtcc aatccaaatt gaaccggtgc ttaaaacgct tctggacgat cagcagacaa
      901 gctatgtttt tgcaattgaa caagaaggca aaacctttgt cggcgcgtct ccggaaagac
      961 tgatcaaaag agacggcggc actgtcatgt cttcctgtct ggcaggctcc attaaacgag
     1021 gcgtgaatga agaagacgac cgccggatag gccttgaatt attaaacgat gagaaaaacc
     1081 tgcttgagca tgatattgtg gtaggtatga ttcataatgc ttttgtatca agctgttcag
     1141 aggttgaaaa gcctgatgga cctgttttgt acaaaacaaa aagcgttcag cacctgttta
     1201 cgccgattgt cggacagctg cgcgagtctg cttcgatttt tgacttaatt gagaagctgc
     1261 accccactcc cgcgcttggg ggatcacctc aggaaaaagc tgttgacgtg atcagggaga
     1321 ttgaaccgat gtcccgcggc tggtatgcgg cccctatcgg ctggattgac agccaggata
     1381 acggggaatt cgcggtcgcc attcgttcag ggctcatcga aggaagcaca gcaaggctgt
     1441 ttgccgggtg cggtattgtg gaagactcgg aaccaatctc ggaatatgaa gaaacacaga
     1501 ttaaattgaa gccgatgatc tctgcattag gaggtgagag gcgttgacag tcaacccgat
     1561 tactcattac atcggaagtt ttattgatga attcgccctt tccggaatta cggacgccgt
     1621 cgtttgtccg ggctcaagat ctacacgctg gctgttcttt gtgctgcgca tcccgatatc
     1681 agcgtccatg tccaaattga tgaaaggtct gccggatttt ttgcattagg tttggcaaaa
     1741 gcgaagcagc gtcccgtact attgatctgt acatctggaa cagccgcggc caacttttat
     1801 ccggctgtgg ttgaagccca ttattcaagg gtgcctatta ttgtgttaac tgctgatcgg
     1861 cctcatgagc tgcgcagagt aggcgcaccg caggctatta atcagcactt cttattcggt
     1921 aactttgtta agtttttcac agactctgct cttcctgaag agtctccgca aatgctgaga
     1981 tacatccgaa cgctggcaag ccgcgctgcc ggagaagcac aaaagcgccc gatggcgctg
     2041 tccatgtgaa tgtgccgctg cgcaaccgct gatgccggat ctttcagatg agccgtttgg
     2101 aagaatgaga acaggccgtc atgtgtctgt gaaaacgggt acgcagtctg ttgaccgaga
     2161 gtccctgtct gatgtggctg agatgctcgc agaggctgaa aaaggaatga tcgtctgcgg
     2221 agagctccat agtgatgcgg acaaggaaaa cattattgcg ctgtcaaaag cgcttcagta
     2281 tccaatttta gcagatccgc tgtctaatct gcggaatggt gttcatgata aaagcactgt
     2341 tattgatgcg tatgattcgt ttttaaaaga tgatgagctg aagcggaaac tccgtcctga
     2401 tgttgtcatt cggtttggac cgatgcctgt ttcaaaaccg gttttcttat ggcttaagga
     2461 tgatccgacg attcagcaaa tcgtgattga cgaggatgga gggtggagag acccgacaca
     2521 ggcaagcgcg catatgattc attgtaatgc gtctgttttt gctgaggaga tcatggcagg
     2581 ccttactgct gccaccagat cctcagagtg gctggaaaag tggcagtttg tcaatgggcg
     2641 gttccgcgag catctgcaaa caatcagcag tgaagatgtt tcgtttgaag gcaatctcta
     2701 tcgaatcctt cagcatcttg tgcctgaaaa cagctcgctg tttgtgggaa acagcatgcc
     2761 gatcagggat gttgatacgt tttttgaaaa gcaggaccgc cctttccgga tttattcaaa
     2821 ccggggagca aacgggatag acggtgtcgt ctcctctgcc atgggggtat gtgaaggaac
     2881 aaaagcgcct gttacccttg tgattggaga tttatctttt tatcatgatt taaacggcct
     2941 gctggcggcc aaaaagctgg gtattcctct tactgtgatt cttgtcaata atgacggagg
     3001 agggattttc tcctttttgc cgcaggcttc tgagaagaca cactttgaag atttgttcgg
     3061 cacaccgaca gggctcgatt tcaagcatgc ggctgcactt tacggcggaa cgtattcatg
     3121 cccggcttct tgggatgaat tcaaaacagc ttacgcgcgc aggcagacaa gcccggactc
     3181 catttgattg aaatcaaaac ggatcgccaa tcaagagtcc agcttcatcg cgatatgctg
     3241 aatgaggctg tgcgggaagt gaaaaaacaa tgggaactgt aaacataacg gtatcagatg
     3301 gtgtccgata tgcagtggca gacgagggac cgaatgcttc cgaagccgtc gtctgtctgc
     3361 atgggtttac cggcagcaaa caatcatgga cttttcttga tgaaatgctg cctgattccc
     3421 gtttgatcaa aattgattgt ttagggcatg gggaaaccga tgccccgctg aatgggaaaa
     3481 gatacagcac aacccggcaa gtttctgacc ttgccgagat ttttgatcaa ttaaaacttc
     3541 acaaagtgaa attgattggg tattctatgg gaggaaggct tgcttactct tttgcgatga
     3601 catatccgag cgggtatcgg cacttgtgct tgaaagcacg acgccggggc tcaaaacact
     3661 tggggaacgg cgggaacgaa tcatgcggga ccggaagctt gctgatttta ttttacgaga
     3721 cgggctcgaa gcgtttgttg cgtattggga gaatatccct ttgttttcat ctcagcagag
     3781 gctggctgaa gatattcggt acaggatacg atcgggccgt ctgcggaaca ataagattgg
     3841 gcttgcgaac agtttaaccg gcatgggcac cggttcacag ccttccttgt ggagccgtgt
     3901 agaggaaata gatgttcctg tgcttctgat ctgcggggag tgggacgaaa aattttgcgc
     3961 catcaatcaa gaggtgcata agatgcttcc ttctagtaga atagagattg tccaaagcag
     4021 gacatacggt ccatgtggag cagccgcgat tggtttggta aaatagtgag tgagttttga
     4081 caagcatctg actcattcac atagagaata aaaggaggtc atcatatggc tgaatggaaa
     4141 acaaaacgga catacgatga agatatttga tgaaacgtat aatggcattg caaaaataac
     4201 aatcaaccga cctgaggtac ataatgcgtt tacccctaaa acggttgctg aaatgattga
     4261 tgcgtttgct gacgcaagag acgaccaaaa cgttggggtt atcgtgcttg ccggtgcagg
     4321 ggacaaagca ttttgttctg gcggagacca aaaagtgcgc ggccacggtg gatatgtagg
     4381 agacgaccag atccctcgtc ttaacgtatt ggatcttcag cgtttaatcc gcgtcatccc
     4441 gaaaccggtt gttgcgatgg tgtccggata tgcgatcggc ggaggccatg tgcttcacat
     4501 cgtatgtgac ttgacaattg ctgcggacaa cgcaattttt ggacaaacag gccctaaagt
     4561 gggaagcttc gatgcaggtt acggttctgg ctacctggct cgaattgtag gacataaaaa
     4621 agcacgtgaa atctggtacc tatgccgtca gtacaacgca caggaagcac tggacatggg
     4681 tcttgtcaac acagtcgttc ctttggaaca gcttgaagaa gaaacgatta aatggtgtga
     4741 agaaatgctt gaaaaaagcc cgaccgcact gcgctttctt aaagctgcgt ttaacgcgga
     4801 cacagacgga cttgctggaa ttcagcagtt tgcaggggat gctacccttc tttactacac
     4861 aacagatgaa gcaaaagaag gccgtgattc ctttaaggaa aaacgcaaac ctgatttcgg
     4921 acagttccct cgttttccgt gatcagcaat atctagtaaa ccaacagctt gagactttgc
     4981 ggtccaagct gttttctttt caatacagac attttacctc ggagatgatg acatgctgac
     5041 agaacagccc aactggctca tgcagcgggc acagctgaca cctgagagaa tcgctctcat
     5101 ctatgaagac caaaccgtga catttgcaga attgtttgcc gcgtctaaac gaatggcgga
     5161 acagcttgcc gctcattcgg ttcggaaagg ggatactgca gctattttgc tccaaaaccg
     5221 tgcagaaatg gtatacgctg ttcacgcttg ttttttgctt ggtgttaagg cggtgctttt
     5281 gaatacgaag ctgtcaacac atgaaaggct gtttcagctg gaggattcgg gatccggctt
     5341 tttattgaca gattcaagct ttgagaagaa agaatatgaa cacatcgttc aaacgattga
     5401 tgtggatgaa ctgatgaaag aagcagcaga ggaaattgag atcgaggctt atatgcaaat
     5461 ggatgcaacg gcaacgctga tgtatacgtc gggtacgaca ggaaagccca agggagttca
     5521 gcaaacgttc ggaaaccatt atttcagtgc gtgtcgtccg ctcttatttg gtataacaga
     5581 acaagacgct ggcttatgca ttgccgctct tcatatcagc ggattgtccg cattatttaa
     5641 atctgtgatc tatggaatga ctgtcgtgct tcaccagcgt ttttccgtaa ccgagtgtgc
     5701 tgcattctat caacattcat gaagtgacaa tgatattcgc ggtgcagact atgctggcca
     5761 gtcttttgga agaaacaaac cgctgccctg aatccatcag atgcattctt ctcggcgcgg
     5821 tcctgcaccg ctgccattgc ttgaggaatg ccgtgagaaa ggattccctg tctttcagtc
     5881 atatggaatg acagaaacat gctcgcaaat tgttaccctg tcgccggaat tc
//