From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: CMC1=carboxymethylcellulase [Cryptococcus flavus=yeast, Genomic,
Date: 3 Jan 93 22:21:27 GMT
Distribution: bionet
Checksum: 50915 5

LOCUS       S115083      1775 bp    DNA             PLN       24-NOV-1992
DEFINITION  CMC1=carboxymethylcellulase [Cryptococcus flavus=yeast, Genomic,
            1775 nt]
ACCESSION   S45137
KEYWORDS    .
SOURCE      yeast
  ORGANISM  Cryptococcus flavus
            Unclassified.
REFERENCE   1  (bases 1 to 1775)
  AUTHORS   Cui,Z., Mochizuki,D., Matsuno,Y., Nakamura,T., Liu,Y., Hatano,T.,
            Fukui,S. and Miyakawa,T.
  TITLE     Cloning and molecular analysis of cDNA encoding a
            carboxymethylcellulase of the yeast Cryptococcus flavus.
  JOURNAL   Biosci. Biotechnol. Biochem. 56, 1230-1235 (1992)
  STANDARD  full automatic
COMMENT     This entry [NCBI gibbsq 115083] was created by the journal scanning
            component of NCBI/GenBank at the National Library of Medicine.
            This sequence comes from Fig. 3.  The authors begin their numbering
            at -274.  Zero is not present in the numbering.
FEATURES             Location/Qualifiers
     CDS             join(275..397,446..543,590..788,840..1073,1121..1221,
                     1270..1374,1425..1590)
                     /note="CMCase 1;  For the protein sequence (NCBI gibbsq
                     115084):  Method: conceptual translation with partial
                     peptide sequencing.  This sequence comes from Fig. 3."
                     /gene="CMC1"
                     /product="carboxymethylcellulase"
                     /codon_start=1
                     /translation="MKTATLLAALSVLAGALAAPLAGDSALHRRSLPRLGGVNLAGCD
                     FGIDIYGNSGTPACPGTEQVGHFIADGANLFRLPAGWQYLVGNNQASTSLAPDFFAQY
                     DALVQAVISKGAYAIIDVHNYARWNGAIIGQGGPSNQDFANLWTLLATKVTSNDPNVI
                     FGLMNEPHDLDVSTWAGSVQAAVNAIRAAGATSQYILIPGTGFTNANAWFQGQDNALL
                     GVTDPVGGTDKLLLDVHRYNDVDFSGTHAECTTNSLDVLSSLDSWLKGNGRKAIVSET
                     GGGHTTSCETDLGEFLNGIKEDYPSVLGFAVWAAGSFDPSYVLSITPTNGVDNQLFDI
                     AVKPNLP"
BASE COUNT      366 a    541 c    497 g    371 t
ORIGIN      
        1 aacgatataa gaagcagttg cagcactgat cagtgggcgt caagccctgc gttcccacat
       61 cgcccgctct tctcatcaca gccctcgctc tatacctcac agaataccga cccgtccgta
      121 ttgacaggtg agtatgctga ctccttgacg cccgcttggt tccagtcggc tgttcctgtc
      181 cttcggttat gggtcgaacc atcatttgcg acctccgagt gggcggtagg cacggacgat
      241 ggattgctga cacgcaggca gattgattat tgagatgaag accgcaacac tgctcgccgc
      301 actgtctgtt ctggctggcg cacttgccgc accactggct ggcgacagtg ccctccatcg
      361 ccgatctctt cctcgtctgg gcggagtcaa cctggccgta agtctggggc gagaagacag
      421 tagattgtag cgactaacaa tgcagggatg cgacttcggt attgatatct acggcaactc
      481 gggcacgcca gcctgccctg gcactgaaca ggtcggccac ttcatcgccg acggcgccaa
      541 cctgtaagtc ctgcccctgc ccctaccgga gcgtaagctc acaccgtagc ttccgcctac
      601 ccgccggatg gcagtaccta gtgggcaaca accaggcgtc gacgtccctc gcgcccgact
      661 tcttcgccca gtatgacgct ctcgtccagg ccgtcatctc caagggagcg tacgccatta
      721 tcgatgtcca caactacgca cgatggaacg gcgctatcat cggtcagggc ggccccagca
      781 accaggacgt acgtgcccaa gggagtatgt gtacttcatg cggctgacga tctgctcagt
      841 ttgccaactt gtggaccttg ctggcgacca aagtaacaag taacgaccca aatgttatct
      901 tcggcctgat gaacgagccg cacgacctcg acgtgtcgac ctgggctggg tccgtccagg
      961 ctgcggtcaa cgcgatccgt gcagccggcg ccacgtcgca gtacattctc atccctggca
     1021 caggctttac caatgcgaat gcatggttcc agggtcagga caacgctctg ctcgtgcgtt
     1081 gatcactgtc gtcagaaccg gtggtagcgc tgactggtag ggcgtgacgg acccggtggg
     1141 cggcacggat aagctcctcc tcgacgtaca caggtacaac gatgttgact tctcgggcac
     1201 ccacgcagag tgcaccacca agttagccag accctagtct ccctctgcct gatcgctgac
     1261 gcgcaccagc tctctcgacg tgctcagctc gctggacagc tggctgaagg gcaacggcag
     1321 aaaggccatc gtgtctgaga ctggcggcgg gcacaccaca tcgtgtgaga cagagtgagg
     1381 tctgtcaggc cctcgcgatt gatcggaacg ctgactcttg acagcctcgg cgagttcttg
     1441 aacgggatca aggaggacta cccttccgtc ctgggctttg cggtctgggc tgctggcagc
     1501 tttgacccaa gctacgtcct atccattacc ccgaccaacg gcgtcgacaa ccagctgttc
     1561 gacatcgctg tcaagcccaa cctgccttga cactaggccc agaagaggag aatgggagaa
     1621 atggaagcat gtcttcagaa agtcaagtct gcgcagcagg tcgcacggct ggatagtcag
     1681 cctcatgaat gtttatggat cctaatatgg ctgcgtttgg cagaagcatc gtcttgcttt
     1741 gattgagtag tctccaggaa tgtggcggcg agctc
//