From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human mRNA for choline kinase.
Date: 29 Jul 92 10:02:23 GMT
Distribution: bionet
Checksum: 24122     5

LOCUS       HUMCK1       2408 bp ss-mRNA            PRI       29-JUL-1992
DEFINITION  Human mRNA for choline kinase.
ACCESSION   D10704
KEYWORDS    choline kinase.
SOURCE      Homo sapiens cDNA to mRNA.
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 2408)
  AUTHORS   Hosaka,K., Tanaka,S., Nikawa,J.-. and Yamashita,S.
  TITLE     Cloning of a human choline kinase cDNA by complementation of the
            yeast cki mutation
  JOURNAL   FEBS Lett. 304, 229-232 (1992)
  STANDARD  full staff_review
COMMENT     Submitted (14-MAR-1992) to DDBJ by:
            Tsutomu Kodaki
            Gunma University School of Medicine
            Department of Biochemistry
            Maebashi, Gunma 371
            Japan
            Phone:  0272-31-7221 x2558
            Email:  tkodaki@ddbj.nig.ac.jp
            Fax:    0272-32-9278
FEATURES             Location/Qualifiers
     CDS             28..1398
                     /EC_number="2.7.1.32"
                     /product="choline kinase"
                     /gene="hgck1"
                     /codon_start=1
                     /translation="MKTKFCTGGEAEPSPLGLLLSCGSGSAAPAPGVGQQRDAASDLE
                     SKQLAPTAALALPPPPPLPLPLPLPQPPPPQPPADEQPEPRARRRAYLWCKEFLPGAW
                     RGLREDEFHISVIRGGLSNMLFQCSLPDTTATLGDEPRKVLLRLYGAILQMRSCNKEG
                     SEQAQKENEFQGAEAMVLESVMFAILAERSLGPKLYGIFPQGRLEQFIPSRRLDTEEL
                     SLPDISAEIAEKMATFHGMKMPFNKEPKWLFGTMEKYLKEVLRIKFTEESRIKKLHKL
                     LSYNLPLELENLRSLLESTPSPVVFCHNDCQEGNILLLEGRENSEKQKLMLIDFEYSS
                     YNYRGFDIGNHFCEWMYDYSYEKYPFFRANIRKYPTKKQQLHFISSYLPAFQNDFENL
                     STEEKSIIKEEMLLEVNRFALASHFLWGLWSIVQAKISSIEFGYMDYAQARFDAYFHQ
                     KRKLGV"
BASE COUNT      568 a    620 c    643 g    577 t
ORIGIN
        1 ccgcgcctcc tcggccgcct gtcgggcatg aaaaccaaat tctgcaccgg gggcgaggcg
       61 gagccctcgc cgctcgggct gctgctgagc tgcggtagcg gcagcgcggc cccggcgccc
      121 ggcgtggggc agcagcgcga cgccgccagc gacctcgagt ccaagcagct ggcgccaaca
      181 gccgcgctcg cgctgccccc tccgccgccg ctgccgctgc cgctgccgct gccccagccc
      241 ccgccgccgc agccgcccgc agacgagcag ccggagcccc gggcgcggcg cagggcctat
      301 ctgtggtgca aggagttcct gcccggcgcc tggcggggcc tccgcgagga cgagttccac
      361 atcagtgtca tcagaggcgg ccttagcaac atgctgttcc agtgctccct acctgacacc
      421 acagccaccc ttggtgatga gcctcggaaa gtgctcctgc ggctgtatgg agcgattttg
      481 cagatgaggt cctgtaataa agagggatcc gaacaagctc agaaagaaaa tgaatttcaa
      541 ggggctgagg ccatggttct ggagagcgtt atgtttgcca ttctcgcaga gaggtcactt
      601 gggccaaaac tctatggcat ctttccccaa ggccgactgg agcagttcat cccgagccgg
      661 cgattagata ctgaagaatt aagtttgcca gatatttctg cagaaatcgc cgagaaaatg
      721 gctacatttc atggtatgaa aatgccattc aataaggaac caaaatggct ttttggcaca
      781 atggaaaagt atctaaagga agtgctgaga attaaattta ctgaggaatc cagaattaaa
      841 aagctccaca aattgctcag ttacaatctg cccttggaac tggaaaacct gagatcattg
      901 cttgaatcta ctccatctcc agttgtattt tgtcataatg actgtcaaga aggtaatatc
      961 ttgttgctgg aaggccgaga gaattctgaa aaacagaaac tgatgctcat tgatttcgaa
     1021 tacagcagtt acaattacag gggattcgac attggaaatc acttctgtga gtggatgtat
     1081 gattatagct atgaaaaata cccttttttc agagcaaaca tccggaagta tcccaccaag
     1141 aaacaacagc tccattttat ttccagttac ttgcctgcat tccaaaatga ctttgaaaac
     1201 ctcagtactg aagaaaaatc cattataaaa gaagaaatgt tgcttgaagt taataggttt
     1261 gcccttgcat ctcatttcct ctggggactg tggtccattg tacaagccaa gatttcatct
     1321 attgaatttg ggtacatgga ctacgcccaa gcaaggtttg atgcctattt ccaccagaag
     1381 aggaagcttg gggtgtgact gtggggagga ctccatccac ctcatcactg gactgcatgg
     1441 ggaggcagca gagcgcggtc ccctctgtgc ttcgactact gctcctgtgg caggaggctt
     1501 tgggtggctc actactgaac acatgtgtat gatactaaag acggtattaa aatggagcga
     1561 cgtttatttc atctcttgtt tacgatttca ctaggactca gaaacgagat cgggaagacg
     1621 aaatatagtg caatagtgca acatctctga atccttttaa tctagagaag gcatttcata
     1681 tttgggggct aaggtttcca gtcagatgag gcaaacagca agagtaagca gtgttacttg
     1741 caggtacttt ggttaatgtt gactttaaat tttcatgaat gtgctggtga acactgtgac
     1801 caggcttttg tagatggcga ctgtgttata gacggtgctc actcccaagg gacagcaagt
     1861 gagcagagat gtactgcaaa gtcgccagtc actgcgtgca aggtggcctc tgcctggggc
     1921 cgtccagaag ctgctccttt accctcttgg tcccatggct gaagcggagc agctggattg
     1981 ctctggagca gccaaggccg ccactgtgga gacagagctc tcccctcctg ctgggcgtgt
     2041 gtgacactgt agagtttcac tgtactcgat gtgacttctc ccctgccctt cctcctgatg
     2101 gagtgtgcag acagccatgc gtggccacgg gggcagtgtg aggacctccc tgtctcccgc
     2161 tcccctccca gggagcagct gcttgaccta gctctttggg cctctcctgc cctctgctct
     2221 gcctggagtg tcggatcctg tgagtaggct gggcctcccc tgggcagggt tctccaaggc
     2281 cggtttcccg gcccttacca aacctgatgc ccctgacatc atcattcttg tgggagacag
     2341 cagcctgtat gtggtgtggg gcgtggatcg agtgtagctg tgaaatccat atatatgaaa
     2401 tgtccaat
//