From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: C.glutamicum gap, pgk and tpi genes for glyceraldehyde-3-phosphate,
Date: 11 Sep 92 10:03:11 GMT
Distribution: bionet
Checksum: 32140     8

LOCUS       CORGAPPGK    3804 bp ds-DNA             BCT       11-SEP-1992
DEFINITION  C.glutamicum gap, pgk and tpi genes for glyceraldehyde-3-phosphate,
            phosphoglycerate kinase and triosephosphate isomerase
ACCESSION   X59403
KEYWORDS    PGK gene; TPI gene; gap gene;
            glyceraldehyde-3-phosphate dehydrogenase; phosphoglycerate kinase;
            triosephosphate isomerase.
SOURCE      Corynebacterium glutamicum DNA.
  ORGANISM  Corynebacterium glutamicum
            Prokaryotae; Firmicutes; Irregular asporogenous rods.
REFERENCE   1  (bases 1 to 3804)
  AUTHORS   Eikmanns,B.J.
  TITLE     Identification, sequence analysis and expression of a gene cluster
            encoding the three glycolytic enzymes glyceraldehyde-3-phosphate
            dehydrogenase, 3-phosphoglycerate kinase and triosephosphate
            isomerase of Corynebacterium glutamicum.
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
COMMENT     *source: strain=AS019;
            Related sequences: X14234 and M25819
            From EMBL    entry CGGAPPGK;  dated 04-SEP-1992.
FEATURES             Location/Qualifiers
     CDS             330..1340
                     /EC_number="1.2.1.12"
                     /product="glyceraldehyde 3-phosphate dehydrogenase"
                     /gene="gap"
                     /codon_start=1
                     /translation="MTIRVGINGFGRIGRNFFRAVLERNGDLEVVAVNDLTDNKTLST
                     LLKFDSIMGRLGQEVEYDDDSITVGGKRIAVYAERDPKNLDWAAHNVDIVIESTGFFT
                     DANAAKAHIEAGAKKVIISAPASNEDATFVYGVNHESYDPENHNVISGASCTTNCLAP
                     MAKVLNDKFGIENGLMTTVHAYTGDQRLHDAPHRDLRRARAAAVNIVPTSTGAAKAVA
                     LVLPELKGKLDGYALRVPVITGSATDLTFNTKSEVTVESINAAIKEAAVGEFGETLAY
                     SEEPLVSTDIVHDSHGSIFDAGLTKVSGNTVKVVSWYDNEWGYTCQLLRLTELVASQA
                     LN"
     terminator      1391..1428
     CDS             1556..2767
                     /EC_number="2.7.2.3"
                     /product="phosphoglycerate kinase"
                     /gene="pgk"
                     /codon_start=1
                     /translation="MAVKTLKDLLDEGVDGRHVIVRSDFNVPLNDDREITDKGRIIAS
                     LPTLKALSEGGAKVIVMAHLGRQGEVNEKYSLAPVAEALSDELGQYVALAADVVGEDA
                     HERANGLTEGDILLLENVRFDPRETSKDEAERNRFAQELAALAADNGAFVSDGFGVVH
                     RAQTSVYDIAKLLPHYAGGLVETEISVLEKIAESPEAPYVVVLGGSKVSDKIGVIEAL
                     AAKADKIIVGGGMCYTFLAAQGHNVQQSLLQEEMKATCTDLLASVDKIVLPVDLVAAS
                     EFNKDAEKQIVDLDSIPEGWMSLDIGPESVKNFGEVLSTAKTIFWNGPMGVFEFAAFS
                     EGTRASPRPSSMQHAGNDAFSVVGGGDSAASVRVLGLNEDGFSHISTGGGASLEYLEG
                     KELPGVAILAQ"
     CDS             2846..3625
                     /EC_number="5.3.1.1"
                     /product="triosephosphate isomerase"
                     /gene="tpi"
                     /codon_start=1
                     /translation="MARKPLIAGNWKMNLDHQQAIGTVQKLAFALPKEYFEKVDVAVT
                     VPFTDIRSVQTLVEGDKLEVTFGAQDVSQHESGAYTGEVSASMLAKLNCSWVVVGHSE
                     RREYHNESDELVAAKAKAALSNGISPIVCVGEPLEIREAGTHVEYVVEQTRKSLAGLD
                     AAELANTVIAYEPVWAIGTGKVASAADAQEVCKAIRGLIVELAGDEVAEGLRILYGGS
                     VKAETVAEIVGQPDVDGGLVGGASLDGEALPKLAANAASVA"
BASE COUNT      876 a   1094 c    991 g    843 t
ORIGIN
        1 gaacgatttc aggttcgttc cctgcaaaaa ctatttagcg caagtgttgg aaatgccccc
       61 gtttggggtc aatgtccatt tttgaatgtg tctgtatgat tttgcatctg ctgcgaaatc
      121 tttgtttccc cgctaaagtt gaggacaggt tgacacggag ttgactcgac gaattatcca
      181 atgtgagtag gtttggtgcg tgagttggaa aaattcgcca tactcgccct tgggttctgt
      241 cagctcaaga attcttgagt gaccgatgct ctgattgacc taactgcttg acacattgca
      301 tttcctacaa tctttagagg agacacaaca tgaccattcg tgttggtatt aacggatttg
      361 gccgtatcgg acgtaacttc ttccgcgcag ttctggagcg caacggcgat ctcgaggtag
      421 ttgcagtcaa cgacctcacc gacaacaaga ccctttccac ccttctcaag ttcgactcca
      481 tcatgggccg ccttggccag gaagttgaat acgacgatga ctccatcacc gttggtggca
      541 agcgcatcgc tgtttacgca gagcgcgatc caaagaacct ggactgggct gcacacaacg
      601 ttgacatcgt gatcgagtcc accggcttct tcaccgatgc aaacgcggct aaggctcaca
      661 tcgaagcagg tgccaagaag gtcatcatct ccgcaccagc aagcaacgaa gacgcaacct
      721 tcgtttacgg tgtgaaccac gagtcctacg atcctgagaa ccacaacgtg atctccggcg
      781 catcttgcac caccaactgc ctcgcaccaa tggcaaaggt cctaaacgac aagttcggca
      841 tcgagaacgg cctcatgacc accgttcacg catacactgg cgaccagcgc ctgcacgatg
      901 cacctcaccg cgacctgcgt cgtgcacgtg cagcagcagt caacatcgtt cctacctcca
      961 ccggtgcagc taaggctgtt gctctggttc tcccagagct caagggcaag cttgacggct
     1021 acgcacttcg cgttccagtt atcaccggtt ccgcaaccga cctgaccttc aacaccaagt
     1081 ctgaggtcac cgttgagtcc atcaacgctg caatcaagga agctgcagtc ggcgagttcg
     1141 gcgagaccct ggcttactcc gaagagccac tggtttccac cgacatcgtc cacgattccc
     1201 acggctccat cttcgacgct ggcctgacca aggtctccgg caacaccgtc aaggttgttt
     1261 cctggtacga caacgagtgg ggctacacct gccagctcct gcgtctgacc gagctcgtag
     1321 cttcccaagc tctaaattaa gttcacatcg ctaacgtggg cgatcgatgc tcacggtgat
     1381 gtgtcatccc aatagcccgg ggtgtgcctc ggcgcacccc gggctatttt gtgtctttaa
     1441 tcaatacaat tgaataccgg ttgccagcgc cacacaatgt gtggccaatc tgggacagtg
     1501 catcacattg caccagaaga attttttaaa caatcaaatc tccaaggagt acggcatggc
     1561 tgttaagacc ctcaaggact tgctcgacga aggcgtagac ggacgccacg tcatcgttcg
     1621 atctgacttc aatgtccccc tcaacgatga ccgcgagatc accgataagg gccgaatcat
     1681 tgcctcccta ccaaccctta aagcactgag cgaaggtggc gcaaaggtca tcgtcatggc
     1741 tcaccttggc cgccaaggcg aggtcaacga gaagtactcc ctcgcacctg tcgctgaggc
     1801 actctccgat gagcttggcc agtacgttgc acttgccgca gacgttgttg gcgaagacgc
     1861 acacgagcgc gcaaacggcc tgaccgaggg cgacatcctg ctcctggaga acgtgcgctt
     1921 cgacccacgc gaaacctcca aggacgaggc agagcgcaac cgtttcgctc aggagctcgc
     1981 agctcttgca gcagacaacg gcgcattcgt ttctgacggc ttcggtgttg tccaccgcgc
     2041 acagacctcc gtctacgaca ttgcaaagtt gctgccacac tacgctggcg gactggtaga
     2101 gaccgagatt tccgttctgg aaaagatcgc agaatcacca gaggcaccat acgtagtggt
     2161 tctcggtgga tccaaggtct ctgacaagat cggtgttatt gaggcgctgg ctgccaaggc
     2221 tgacaagatc atcgtcggtg gcggcatgtg ctacaccttc ctcgcagctc agggacacaa
     2281 cgttcagcag tccctcctgc aggaagaaat gaaggctacc tgcaccgacc tgctcgcttc
     2341 ggtggacaag atcgttctcc cagttgacct ggttgcagca tccgaattta acaaggacgc
     2401 agagaagcag atcgttgacc tggactccat cccagaaggc tggatgtctc ttgacatcgg
     2461 accagagtcc gtcaagaact tcggtgaggt tctcagcacc gctaagacca tcttctggaa
     2521 cggcccaatg ggcgtgttcg agttcgcagc attctctgaa ggcacccgcg catcgcccag
     2581 gccatcatcg atgcaacatg caggcaacga cgcattctcc gttgttggcg gtggcgactc
     2641 cgcagcatcc gttcgcgtgc tcggcctgaa cgaagacggc ttctcccaca tctccaccgg
     2701 tggtggcgca tccctcgagt accttgaagg caaggaactc ccaggcgttg caattctcgc
     2761 tcagtaaatc cgacacggcc ctttgttaga aaacaaaaca taaagggcca ccgggaaact
     2821 tttttaagaa aggtgtgttt cacacatggc acgtaagcca cttatcgctg gtaactggaa
     2881 gatgaacctg gatcaccagc aggcaatcgg cactgttcag aagcttgcat tcgcccttcc
     2941 aaaggaatac ttcgagaagg ttgacgttgc agtcaccgtt cctttcactg acatccgctc
     3001 cgtccagact ctcgttgagg gcgacaagct tgaggtcact ttcggtgctc aggacgtctc
     3061 ccagcacgag tccggtgcgt acaccggtga agtttctgca agcatgctgg caaagttgaa
     3121 ctgctcttgg gttgtcgttg gacactccga gcgccgcgag taccacaacg agtctgatga
     3181 gttggttgct gcgaaggcaa aggcagctct gtccaacggc atcagcccga tcgtctgcgt
     3241 tggtgagcca ctggaaatcc gtgaagctgg cacccacgtt gagtacgtcg tcgagcagac
     3301 ccgtaagtcc cttgctggcc tggatgctgc tgagctggcc aacaccgtta tcgcgtatga
     3361 gccagtgtgg gctatcggca ccggtaaggt tgcttccgca gctgacgctc aggaagtgtg
     3421 caaggctatc cgcggtctga tcgtggagct tgcaggcgac gaggtcgctg agggcctgcg
     3481 tattctttac ggtggttctg ttaaggcaga aaccgtcgct gagatcgtcg gtcagcctga
     3541 cgtcgacggc ggacttgtcg gtggcgcttc cctcgacggt gaagcattgc ccaagctggc
     3601 tgccaacgct gcgagcgttg cttaaagtac agagctttaa atcacagcct taaagcacag
     3661 ccttaaagca caagcactgt agaagtgcgg ttttgatgag cccatgaaag ccatcgaaat
     3721 caatcgccca gctaaacacc tgttttgctg ggtgattttt tatctcatgc acgccaacac
     3781 cctcaatgtg aaagagtgtt taaa
//