From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: D.pseudoobscura Adh gene for alcohol dehydrogenase
Date: 28 Dec 92 21:27:39 GMT
Distribution: bionet
Checksum: 47821 8

LOCUS       DROADHGN59   3476 bp ds-DNA             INV       01-DEC-1992
DEFINITION  D.pseudoobscura Adh gene for alcohol dehydrogenase
ACCESSION   X64468
KEYWORDS    ADH gene; alcohol dehydrogenase.
SOURCE      Drosophila pseudoobscura DNA.
  ORGANISM  Drosophila pseudoobscura
            Eukaryota; Animalia; Metazoa; Arthropoda; Uniramia; Insecta;
            Pterygota; Neoptera; Holometabola; Diptera; Brachycera;
            Cyclorrhapha; Schizophora; Drosophiloidea; Drosophilidae.
REFERENCE   1  (bases 1 to 3476)
  AUTHORS   Schaeffer,S.W. and Miller,E.L.
  TITLE     Estimates of gene flow in Drosophila pseudoobscura determined from
            nucleotide sequence analysis of the alcohol dehydrogenase region
  JOURNAL   Genetics 132, 471-480 (1992)
  STANDARD  full automatic
COMMENT     *source: strain=PS163; *source: isolate=Stemwinder Provincial Park,
            B.C. Canada; *source: chromosome=IV section 88;
               From EMBL    entry DPADHGN59;  dated 16-OCT-1992.
FEATURES             Location/Qualifiers
     mRNA            join(40..119,915..1020,1085..1489,1550..1967)
                     /note="adult mRNA (Adh)"
     mRNA            join(882..1020,1085..1489,1550..1967)
                     /note="larval mRNA (Adh)"
     promoter        8..14
                     /note="adult promoter (Adh)"
     prim_transcript 40..1967
                     /note="adult primary transcript (Adh)"
     exon            40..119
                     /note="adult exon 1a"
     intron          120..914
                     /note="adult intron"
     prim_transcript 882..1967
                     /note="larval primary transcript (Adh)"
     exon            882..1020
                     /note="larval exon 1"
     exon            915..1020
                     /note="adult exon 1b"
     intron          1021..1084
                     /number=1
     exon            1085..1489
                     /number=2
     intron          1490..1549
                     /number=2
     exon            1550..1967
                     /number=3
     polyA_signal    1944..1949
     polyA_site      1967
     CDS             join(928..1020,1085..1489,1550..1816)
                     /EC_number="1.1.1.1"
                     /gene="Adh"
                     /product="alcoholdehydrogenase"
                     /codon_start=1
                     /translation="MSLTNKNVVFVAGLGGIGLDTSRELVKRNLKNLVILDRIDNPAA
                     IAELKAINPKVTITFYPYDVTVPVAETTKLLKTIFAQVKTIDVLINGAGILDDHQIER
                     TIAVNYTGLVNTTTAILDFWDKRKGGPGGIICNIGSVTGFNAIYQVPVYSGSKAAVVN
                     FTSSLAKLAPITGVTAYTVNPGITKTTLVHKFNSWLDVEPRVAEKLLEHPTQTSQQCA
                     ENFVKAIELNKNGAIWKLDLGTLEPITWTQHWDSGI"
BASE COUNT      969 a    790 c    798 g    919 t
ORIGIN      
        1 cgtagtgtat ttaaggcact gcacatgtcg aggaccacga ttattgtcta cagtgcagtc
       61 agtagttgtc aggtgcagca ggcggaccag catttcttta aatctactca atcgatcaag
      121 taagtaactg taatccgagt acagtcgacc catcccactc ggttctgcag taactcaaga
      181 aatttcttga tagcgcagtt ccatttgtag aacgtcgttt aaaaaatcat ctcgttggag
      241 cttgggttgg aaaacgttcg agttgggcgt agaaaagttc aaatataaaa tatcaaaaaa
      301 gagaaaatat cttcccaaag cagctgtcca ttttttgtta aagtgataga tcaaaaattc
      361 gtatgacgta tttcccaagt gctctttgtg caaataattt cctcaattct aattctacaa
      421 gaagcacttt tggctcttgt ccagaaaaaa caatttgttt acacttgtcg gatgataaca
      481 gaagcggacc gacggctaag taacataaca gtcgcctgtt aagtaatgcc ctcactctct
      541 gttagattgc cgcgcgtgtc gactccgata gaccagcaga cacgcagtag ttttcctcaa
      601 gaacctgaac tctaaacata gacataattt acgatacctt gagactcaaa taaaaataca
      661 agtatgagtg gtattctatt cctgattaga ctcgcacaca cacacgtacg agtattaaca
      721 taaatttgcc ggcaaataac caaaacaaaa cgaacgcaaa cgatccgaac acgtaatgcg
      781 agagataaga aacagaaaag ctcgacgtga aagaagcttc tggcggaagc tcttgccgaa
      841 ctccctataa atacgagaca gaaaaccaga ataaatataa taacaaactg ttgaattgtc
      901 ccatcgattc ccagatcaaa agacagaatg tctctcacca acaagaacgt cgttttcgtg
      961 gccggtctgg gcggcattgg cctggacacc agccgggagt tggtcaagcg taatctgaag
     1021 gtaagagaga acgaattcca tggaatctat ggaatcctaa atttcaaaaa ttcatttatt
     1081 ttagaacctg gtcatcctgg atcgcattga caatccggct gccattgccg aactgaaggc
     1141 aatcaatccc aaggtgacca tcaccttcta tccctacgat gtgactgtgc ccgtcgctga
     1201 gaccaccaag ctcctgaaga ccatctttgc ccaggtgaag acaatcgatg tcctgatcaa
     1261 cggtgctggc atcctggacg atcatcagat cgagcgcacc attgccgtta actacacggg
     1321 cctggtcaac accaccacag ccattctgga cttctgggac aagcgcaagg gcggcccagg
     1381 cggcatcatt tgcaacattg gctccgtcac cggtttcaat gccatctacc aggtgcccgt
     1441 ttactccggc tccaaggcgg cggtggttaa ctttacctcc tccctggcgg taagcaatcc
     1501 aacgtttttc ctactcccca aaactaaata tttctttgaa atcccataga aactggctcc
     1561 cattactggt gtcactgctt acactgtcaa tcctggcatc actaagacca ctctggtcca
     1621 caaattcaac tcgtggctgg atgtggagcc ccgtgtggcg gagaagctgc tcgagcatcc
     1681 cacccagacc tctcagcagt gcgccgagaa ctttgtgaag gccattgagc tgaacaagaa
     1741 cggtgccatc tggaagttgg atctgggcac cttggagccc atcacatgga cccagcactg
     1801 ggactcgggc atctaaagat catcccagag actctatggg acatggcgtt ttagctttta
     1861 gtttcttttt ttccactcaa ttgttacgta tatacataca catatggcaa taaggctgat
     1921 ttgaaccccg ttttgaatat gataatacaa aattatattt gagaaatttc aacaaaatcg
     1981 ataagagcta tagctctctt ttaattaaaa aaaactagat aaaaagagca atggtcagtg
     2041 gtggtcagtg gtcgttgtgg cctctatcga tttccacaca aaaactttac tgtttagtaa
     2101 tagaaaagaa cttgagaaag gcagccaaaa gaatgtacga tctgacgggt aagcatgtct
     2161 gctatgtggc tgactgcggt ggcattgcac tggagactag caaggttctc atgaccaaga
     2221 atatagcggt gagtggtaga gtggtgtaga gtgtggagag tggttctggt tcttaaacaa
     2281 gtctctagtc tctagtctct agtctcttgg ctgctgctgg tagctgtcga aactaattaa
     2341 gttaatgact ttttcatgtt ttgtttttgt ccgctgactt ttgctgttca aagctacaat
     2401 taagtttatg ttttgagttt gatctacttt tgcttaaggg aagtactttc gattacggat
     2461 tgctggcata taaaaataga taccaaggaa tgtgcatcat atcatattga ttctatcttg
     2521 attctttgcc cagaaactgg ccatccttca gagcgtggaa aaccccccgg ccatcgctca
     2581 gttacaatcg attaagcaca gcacacagat cttcttctgg accttcgatg tgaccatggc
     2641 ccgcgaggag atgaagaagt acttcgatga ggtcatggtc cagatggact acatcgatgt
     2701 gctgatcaat ggggccaccc tgtgcgatga gcggaacatt gatgccacca tcaatacgaa
     2761 tctgaccggc atgatgaaca ccgtggccac tgtgctgccc tatatggacc gtaagatggg
     2821 cggttcgggt ggactgattg tgaatgtcac ctccgtcata ggactggatc catcgccagt
     2881 cttttgtgcg tacagtgcct caaagtttgg tgtgattggg ttcacccgaa gtctagcggt
     2941 gagtgggcga gatcttagct tgaaaatgct ctctaatgtc ttccattctt tcgcaggatc
     3001 ccctgtatta cacccaaaat ggtgtggctg tcatggccgt ctgctgtggt cccaccaaag
     3061 tgtttgtcga tcgggaactg actgccttcc tggcatacgg ccaaaccttt gccgatcgcc
     3121 tgcgtcgtgc cccctgccaa tcgacggccg tctgcggcaa gaatatagta aatgccattg
     3181 agagatcgga gaacggacag atctggattg ccgacaaggg cggcctggag tcggtggccc
     3241 tccactggta ttggcatatg gccgatcagt ttgtgaacta tatgcagagc actgacgatg
     3301 aggatcagga gttttttttg gggcagcgat gaggcatgcc ttgatccacg gaataaaaca
     3361 tcaaatgtgt cacaaaaagt aggcaacttg tttgtgggtc tggaaataga tttgaaatat
     3421 tttgaggtca tatgaataat gacataaatg agggtgcctg aaagtatgcc aagttt
//