From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Bacillus subtilis cwlA gene for cell wall hydrolase
Date: 29 Dec 92 00:26:23 GMT
Distribution: bionet
Checksum: 49090 3

LOCUS       BACCWLAGE    1128 bp ds-DNA             BCT       03-DEC-1992
DEFINITION  Bacillus subtilis cwlA gene for cell wall hydrolase
ACCESSION   X51424 M37710 M60054
KEYWORDS    cell wall hydrolase; cwlA gene; hydrolase.
SOURCE      Bacillus subtilis DNA.
  ORGANISM  Bacillus subtilis
            Prokaryotae; Firmicutes; Endospore-forming rods and cocci;
            Bacillaceae.
REFERENCE   1  (bases 1 to 1128)
  AUTHORS   Kuroda,A. and Sekiguchi,J.
  TITLE     Cloning, sequencing and genetic mapping of a bacillus subtilis cell
            wall hydrolase gene
  JOURNAL   J. Gen. Microbiol. 136, 2209-2216 (1990)
  STANDARD  full automatic
COMMENT     *source: strain=168S;
               From EMBL    entry BSCWLAGE;  dated 16-NOV-1992.
FEATURES             Location/Qualifiers
     -35_signal      17..22
     -10_signal      40..45
     repeat_unit     55..81
                     /rpt_type=inverted
     RBS             97..107
     repeat_unit     941..967
                     /rpt_type=inverted
     CDS             114..932
                     /gene="cwlA"
                     /product="hydrolase"
                     /codon_start=1
                     /translation="MAIKVVKNLVSKSKYGLKCPNPMKAEYITIHNTANDASAANEIS
                     YMKNNSSSTSFHFAVDDKQVIQGIPTNRNAWHTGDGTNGTGNRKSIGVEICYSKSGGV
                     RYKAAEKLAIKFVAQLLKERGWGIDRVRKHQDWNGKYCPHRILSEGRWIQVKTAIEAE
                     LKKLGGKTNSSKASVAKKKTTNTSSKKTSYALPSGIFKVKSPMMRGEKVTQIQKALAA
                     LYFYPDKGAKNNGIDGVYGPKTADAIRRFQSMYGLTQDGIYGPKTKAKLEALLK"
BASE COUNT      413 a    186 c    239 g    290 t
ORIGIN      
        1 gatcgattac aaacaattga gaacgaaaaa gaacagagta agaataacgc agacaaagct
       61 gctggctaag ccagtggctt tttttatctt actgacagaa ggagagaggg tatatggcca
      121 ttaaagttgt aaagaatcta gtctctaaat caaagtatgg attgaaatgt cctaatccaa
      181 tgaaagctga atatatcact attcataaca ctgcgaatga tgcttcagca gccaatgaga
      241 tttcttacat gaagaataac tctagctcaa caagttttca ctttgcagta gacgataaac
      301 aagtcattca aggtattcca acgaatcgta acgcttggca cacaggagat ggaacaaacg
      361 gtacagggaa tcgcaagtct attggtgtcg aaatttgtta tagcaagtca ggaggggtac
      421 gatataaggc agcggaaaag cttgctatta agtttgtggc tcagctactt aaagaacgtg
      481 gatggggtat tgatcgagtc cgcaaacatc aagactggaa tggtaagtat tgcccgcacc
      541 gcattttgtc agagggaaga tggattcaag ttaagactgc aattgaagca gaattgaaaa
      601 agttgggcgg aaaaacaaac tcaagcaaag caagtgtagc taaaaagaaa acaacaaaca
      661 caagcagcaa aaaaacgtca tatgcgctac catccggtat ttttaaagtg aagagcccaa
      721 tgatgagagg ggaaaaggta acacaaattc aaaaagcact ggctgcacta tacttttacc
      781 cggataaagg agcaaaaaac aacggcattg acggcgtgta tggtccgaaa acagcagatg
      841 caattagacg attccagtct atgtacgggc ttactcaaga cggtatttac ggaccaaaaa
      901 cgaaagcgaa acttgaagct ctcttgaagt aaataaatag tctccttgag tatctttctc
      961 aaggagatac ttatgtttat tttgttacag atagttcaaa attattctaa tatttgagta
     1021 aaaagtatta tttttctagt ctgaacataa tgttatcatt tacatgtaaa taatttaaaa
     1081 gaaagggatt gatttcatgt ttaagaaatt acttttagca acatctgc
//