From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: E.chrysanthemi genes outS, outB, outC, outD and outT
Date: 15 Aug 92 10:01:45 GMT
Distribution: bionet
Checksum: 20958    12

LOCUS       ERWOUTDNA    6066 bp ds-DNA             BCT       15-AUG-1992
DEFINITION  E.chrysanthemi genes outS, outB, outC, outD and outT
ACCESSION   X65265
KEYWORDS    outB gene; outC gene; outD gene; outE gene; outS gene; outT gene;
            pectate lyase secretion.
SOURCE      Erwinia chrysanthemi DNA.
  ORGANISM  Erwinia chrysanthemi
            Prokaryotae; Gram-negative facultatively anaerobic rods;
            Enterobacteriaceae.
REFERENCE   1  (bases 1 to 6066)
  AUTHORS   Condemine,G., Dorel,C., Hugouvieux-Cotte-Pattat,N. and
            Robert-Baudouy,J.
  TITLE     Some of the out genes involved in the secretion of pectate lyases
            in Erwinia chrysanthemi are regulated by kdgR
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
REFERENCE   2  (bases 1 to 6066)
  AUTHORS   Condemine,G.
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
COMMENT     *source: strain=3937;
            The out genes are required for the translocation of pectate lyases
            and cellulases across the outer membrane
            From EMBL    entry ECOUTDNA;  dated 06-AUG-1992.
FEATURES             Location/Qualifiers
     CDS             311..670
                     /gene="outS"
                     /codon_start=1
                     /translation="LPESGGLSDAGAGEKHCFRSAASVPANEQISQLASLVAASKYLR
                     VQCERSDLPDDGTILKTAVNVAVQKGWDTGRYQSLPQLSENLYQGLLKDGTPKATQCS
                     SFNRTMTPFLDAMRTVR"
     CDS             complement(705..1178)
                     /gene="outB"
                     /codon_start=1
                     /translation="MVPASTAVTADENHPGSLHAAAENSATQTAASGSQTSASSQEAT
                     PDTKPAKLVTGWQTAKPGELPYIAFSAHVYTSAPDKRSVTLNGERYREGDSPYQGLVI
                     EQIEQDMVIFSFNGEPFILDSLQDWPGGKPGDDAAQGNEQEPTSKPEQTVRTTKK"
     CDS             1733..2098
                     /pseudo
                     /gene="outT"
     CDS             2827..3645
                     /gene="outC"
                     /codon_start=1
                     /translation="MNISKLPPLSPSVIRRILFYLLMLLFCQQLAMIFWRIGLPDNAP
                     VSSVQITPAQARQQPVTLNDFTLFGVSPEKNKAGALDASQMSNLPPSTLNLSLTGVMA
                     GDDDSRSIAIISKDNEQFSRGVNEEVPGYNAKIVSIRPDRVVLQYQGRYEVLGLYSQE
                     DSGSDGVPGAQVNEQLQQRASTTMSDYVSFSPIMNDNKLQGYRLNPGPKSDSFYRVGL
                     QDNDMAVALNGLDLRDAEQAKKAMERMADVHNFTLTVERDGQRQDIYMEFGGDE"
     CDS             3647..5779
                     /gene="outD"
                     /codon_start=1
                     /translation="MLGKGIKKSWGWLGLTVLLLGSPCGWAAEFSASFKGTDIQEFIN
                     TVSKNLNKTVIIDPTVRGTISVRSYDMMNEGQYYQFFLSVLDVYGFSVVPMDNGVLKV
                     IRSKDAKSSSIPLANNEQPGIGDELVTRVVPLNNVAARDLAPLLRQLNDNAGAGTVVH
                     YEPSNVLLMTGRAAVIKRLVDIVNTVDKTGDREMVTVPLTYASAEDVAKLVNDLNKSD
                     EKNALPSTMLANVVADGRTNSVVVSGEENARQRAVEMIRQLDRKQVVQGGTKVIYLKY
                     AKALDLIEVLAGNGTSGNRNSSSSNASRPSSPRSGSSSNSNSSSGSSGSSSGSSSSSS
                     SSSSMGFGSAFGSTSSSGGRTITIQGKEVTVRAHDQTNSLIITAPPDIMRDLEQVINQ
                     LDIRRPQVLVEAIIAEIQDADGLNLGIQWANKRAGMTQFTNTGIPISTAVIGTDQFRS
                     NGTLTTAYASALSSFNGVTAGFYRGNWSMLLTALSSDSKNDVLATPSIVTLDNMEATF
                     NVGQEVPVLTGSQTTSADNIFNTVERKTVGIKLRVKPQINEGDSVLLQIEQEVSSVAD
                     SNSSTNSSLGVTFNTRTVNNAVMVTNGETVVVGGLLDKTSVESNDKVPLLGDIPWLGS
                     LFRSKSQEVRKRNLMLFLRPTIIRDPGQFQEASINKYRSFNNEQQQQRGEGNGVLDNN
                     TLRLSGGNTYTFRQVQSSISDFYKPEGR"
     CDS             5776..>6066
                     /gene="outE"
                     /codon_start=1
                     /translation="MSDQPVNTPELRPVLPFSFARAHEILLLQDESVTVAEVVCVPDT
                     PALALLEARRVAGVPLAVSQVSPEEFERQLVMRYQRDSEEARRLMEDIGNDID"
BASE COUNT     1449 a   1500 c   1712 g   1405 t
ORIGIN
        1 gtgcgcttcg cattgattga aaatcaggat cgcattcggc aggcggtgcg cggcatcaaa
       61 gccatgttcc gggctgacgg cgtgctgtcc ggcagtaaaa agggcgagta agcccgccgg
      121 gcggacttgc agaaatattt ttctgatggt ttgatcacgc agtcctttgc cgccgcttgt
      181 ggtggcaaag ggataatgat ttgctaaacg aacgcccaag ccgggcatcc cagagatgga
      241 acttaatcaa taataagacg agtcgagtca tgcatgtatc ttcgctaaaa gtggtccttt
      301 ttggtgtctg ttgcctgagt ctggcggcct gtcagacgcc ggcgccggtg aaaaacactg
      361 cttccgcagc gcggccagcg tgccggccaa tgagcaaatc agccagttgg cgtcgttggt
      421 ggcggccagt aaataccttc gggtgcagtg tgaacgcagc gatttgccgg atgacggcac
      481 aatactgaaa acggcggtga acgtagcagt gcaaaaagga tgggataccg gtcgttatca
      541 atctttgccg caattgagcg agaacctgta tcaggggctg ctgaaagacg gcacgccgaa
      601 agcgacgcag tgctcttcat ttaatcgcac catgacgccg tttcttgacg ccatgcgcac
      661 cgttcgctga gggggaagca actgcatgat gtgcagttgc tgtgtcattt cttcgttgtc
      721 ctgacggttt gctccggttt tgacgtcggc tcctgctcat tgccttgcgc ggcgtcatcg
      781 cccggtttcc cgcccggcca atcctgcaac gaatccagaa taaacggttc gccgttgaag
      841 ctgaagatca ccatatcctg ctcaatctgc tcgatcacca acccctgata cgggctgtcg
      901 ccttcacggt agcgctctcc gttcagggta acgctgcgct tgtccggagc agacgtatag
      961 acatgcgcgc tgaaggcgat atacggcagc tcaccgggtt tggccgtttg ccagcctgtt
     1021 accaatttgg cgggttttgt gtctggcgtc gcctcctgtg aggaagctga tgtttgacta
     1081 cccgatgctg ccgtctgagt tgcgctgttt tctgcggcgg catggagcga accaggatgg
     1141 ttttcatccg cagtgaccgc tgttgatgcc ggaaccatgc ccactttggt aggcggtgcg
     1201 gcgatcgccg ccgcagaggt cgacagcgga atgggctgat atcggcccag cgttgatgac
     1261 caaaccaacc cagcgtaaac agcagcaagg catagacaac cagcaagtaa ccaggaatac
     1321 ggtagccggt ctggggagat gctttaactt ccggtgtatt tttcacgtct tacctcaaac
     1381 gttatacaac ctgacgctta tcctgagcgt accataacga accttgacgg aacgtctgat
     1441 gtctcatttt tcaggaaaag agaaggaata atcaacgtct tatcccattt atgtgacctg
     1501 gatgccattt caacaacatt ctattacgtt gatcattaat gccggtaaaa ttttgagcct
     1561 ggtcgcaaaa aaaagttggg attatgtttt aatgaaacgg tgttttatta ttgtgctatt
     1621 actgcttgat aaatagcctc gcacattgat acttcaccat tagtcatacc atagcctaat
     1681 gttggcggaa aatggtggta aatatcgtta aaaaacaatg cgaaagatat tggtgaagca
     1741 ttgctgtcgc gcgttcccaa aggcaagaat agcgctattg cgccaggctt ttgggctaca
     1801 aacagcataa caagtacatg ataagcaagt atgtggggaa caaactatat tcgaaatagt
     1861 tcacgttatc caaaatagtt cacgttgcgg gaagccacaa ccggtaaata cccagggact
     1921 tgctcaggta ggtgacagcg aggacgaaca atacaccagc agcttgaaat atggcctgca
     1981 tatcatcctg ccactcgatg atgccagtct cataacgcca ggtgaggtga agttttgtca
     2041 ctggagaatg gtcaaccacg agggagcagg tgaacgacat tatgcatgat cactgtaata
     2101 aatccccgtg gtaattaatg gtggcgcagt ggcctttccg gtgtgattta ttatgagatg
     2161 ttaatactat tggttatgta atgttaagcg ggatatgacg tctgaatttg caaatgtaac
     2221 gtcatgctat cgaatcaacc cattaattat tattgttttt ccctgtgcag atagccccga
     2281 cagaaataga atattgcatt atgtacaggt atggtgcgta aatgaaaagt aattccatat
     2341 tacgtggtgt tgcattgacg tgagttcaat agttaccgaa agcattttac ggttaatgaa
     2401 aatagtggga gaaaatggtt gctgactacg ggattgcctg tcattaacct gagttatatt
     2461 actgagcgct tttagcctta tccagctcat gtagcgggtg cgaatcgcct ggtgcaacgg
     2521 gaaacaggat gcctgtcaac cagtgacacg attagagcgg ctgggagcac gcgcggcggg
     2581 ctgaaatcgg ccagagcatg aatatcgtga tggttttttg tcgtcagcct gatgtatcgg
     2641 ttttgcgatg ataccatcac cgttgctccg acaatccggc cgatatggcg ataaggcagc
     2701 gaaaatgcag atgcgtcgtt tttcggaaca cggcaacacg tttgaaaatg gcgctatatg
     2761 gcccttactc cacgtttacg gacgtggcaa ggataatcgg cgaacacata gggataatgt
     2821 ttgcagatga atatctcgaa attgccaccg ctatctccgt ctgtcatcag acggatttta
     2881 ttttacctgc tgatgctgct cttttgtcag cagctggcaa tgatcttctg gcgtattggt
     2941 ttaccggaca atgcgccggt ctccagcgta cagatcaccc cggcacaggc cagacagcag
     3001 ccggtcaccc tgaatgattt tacgctattc ggcgtttctc ccgaaaaaaa taaggcgggc
     3061 gcgctggatg cgtcgcaaat gagcaatctg cctccttcta cgctgaacct ttcgctgacc
     3121 ggcgtcatgg ccggcgatga cgattcccgt tctatcgcca tcatcagtaa agacaacgaa
     3181 cagttcagtc gtggcgtcaa tgaagaggtt cctggctata acgccaaaat cgtatcgatc
     3241 cggccggata gagtggtgct gcagtatcaa gggcgttatg aagtgctggg attgtacagc
     3301 caggaggata gcgggtcgga cggcgttcct ggcgcgcagg tcaacgagca gcttcagcag
     3361 cgcgcatcca ccacgatgag cgattacgtc tctttctccc cgatcatgaa cgacaacaag
     3421 ttgcagggct accggttgaa tccggggccg aagagcgatt cattctaccg cgtcgggctt
     3481 caggataacg atatggccgt tgccctgaac gggttggatt tgcgcgatgc ggagcaggcg
     3541 aaaaaagcca tggaacgcat ggctgatgtg cataacttta ccctgacggt ggagcgggat
     3601 ggtcagcgtc aggatattta tatggaattt gggggagacg agtaacgtgc ttggcaaagg
     3661 gatcaaaaaa agctgggggt ggctggggct aaccgttctc ctgcttggct cgccgtgtgg
     3721 ctgggctgcc gaattttcag ccagtttcaa aggaaccgat attcaggagt tcatcaatac
     3781 agtcagtaaa aacctgaaca aaacggtgat tattgacccg acggtgcgcg gcaccatcag
     3841 cgtgcgcagt tacgacatga tgaacgaagg gcagtattac cagtttttcc tcagtgtgct
     3901 ggatgtgtat ggcttttcgg tagtgccgat ggataacggc gtattgaaag tcattcgttc
     3961 caaagatgct aaatcgtcct ccattccgct ggccaataac gaacaaccgg gcattggtga
     4021 tgagctggtg acccgcgtgg taccgctcaa taatgtggcg gcgcgcgatc tggctccgtt
     4081 gctgcgtcag ctcaacgata acgccggcgc cggcaccgtg gtgcattatg aaccgtccaa
     4141 cgtcctgctg atgaccgggc gtgccgccgt gatcaaacgg ctggtggata tcgtcaatac
     4201 ggtggacaag accggcgatc gtgaaatggt caccgtccca ttgacctatg cgtcggcgga
     4261 ggatgtcgcc aaactggtga acgatcttaa caagagcgat gagaagaacg cgttgccaag
     4321 caccatgctg gctaacgtgg tggcggatgg ccgcaccaac tctgtggtgg tgagcgggga
     4381 agaaaacgcc cgccagcgcg cggtggaaat gatccgccag ttggatcgca agcaggtggt
     4441 gcagggcgga accaaggtta tctatctcaa gtacgccaag gcgctggatc tgattgaagt
     4501 actggccggc aacggaacca gcggcaatcg caattcgtcg tcgtcgaatg cgtcgcggcc
     4561 gtcctcgccc cgttccggca gtagctcaaa cagcaacagc agctccggtt cgtccggcag
     4621 ctcttccggc agttcttctt cgtcgtcgtc cagttccagt atggggtttg gctcggcatt
     4681 tggttcgacc agcagttcag gtggtcgcac tatcacgatt cagggcaaag aagtgacggt
     4741 tcgcgcccat gatcagacca actcgctgat cattaccgcg ccccccgata tcatgcgcga
     4801 cctggagcag gtgatcaatc agctggatat tcgccgtccg caggtgctgg tggaagcgat
     4861 cattgccgaa attcaggatg ccgacgggct gaatctgggg atccagtggg ccaacaagcg
     4921 cgccggcatg actcaattca ccaatacggg cataccgatt tcaacggcgg tgatcggaac
     4981 cgatcagttc cgcagcaatg gcacgctgac gacggcgtat gccagcgcgc ttagcagctt
     5041 caacggcgtt accgccggtt tttatcgcgg caactggtcc atgctgctga ccgcgttgtc
     5101 cagcgatagc aaaaacgatg tgctggcgac gccgagcatt gtgacgctgg ataacatgga
     5161 agcgaccttc aatgtgggtc aggaagtgcc ggtcctgacc ggttcacaga ccacgtcggc
     5221 cgacaacata tttaataccg tcgagcgtaa gaccgtcggc atcaaactgc gggtaaaacc
     5281 gcagatcaac gaaggcgact cagtgttgct gcaaattgag caggaagtgt ccagtgtcgc
     5341 ggacagcaat agcagtacca acagttcact gggcgtgacc ttcaacaccc gtaccgtgaa
     5401 caatgcggtg atggtcacca acggtgaaac cgtggtagta ggcggcctgc tggataaaac
     5461 ctccgttgaa tctaatgaca aagtaccgtt gctgggcgac attccgtggc tgggtagcct
     5521 gtttcgttcg aaaagtcagg aagtgagaaa acgcaacctg atgctgttcc tgcgtccgac
     5581 cattattcgc gacccagggc agttccagga agcgtcgatc aacaaatacc gttccttcaa
     5641 caatgaacag cagcaacagc gcggcgaagg caacggggtg ctggataaca acaccctgcg
     5701 tttatccggt ggcaacacgt acaccttccg tcaggtgcag tcgtccatct ccgactttta
     5761 taagccggag gggcgatgag cgatcagccc gtcaacacgc ctgaactgcg accggtgctg
     5821 ccgttttctt ttgcccgggc gcacgagatt ctgttgttgc aggatgagtc ggtgacagtg
     5881 gcggaagtcg tttgtgtacc cgatacacca gcgctggcgt tgttggaagc gcgccgggtc
     5941 gccggggtgc cgttggcggt gagccaggtc agtccggaag agtttgagcg gcaactggtg
     6001 atgcgttatc agcgcgactc cgaagaagcg cgccgcctga tggaggacat cggtaacgat
     6061 atcgat
//