From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: P.syringae gene for ethylene-forming enzyme, complete cds.
Date: 9 Jan 93 05:47:31 GMT
Distribution: bionet
Checksum: 24232 5

LOCUS       PSEETH       1809 bp ds-DNA             BCT       09-JAN-1993
DEFINITION  P.syringae gene for ethylene-forming enzyme, complete cds.
ACCESSION   D13182
KEYWORDS    ethylene-forming enzyme.
SOURCE      Pseudomonas syringae (variety phaseolicola PK2) DNA.
  ORGANISM  Pseudomonas syringae
            Prokaryotae; Bacteria; Gracilicutes; Scotobacteria;
            Pseudomonadaceae.
REFERENCE   1  (bases 1 to 1809)
  AUTHORS   Fukuda,H., Ogawa,T., Ishihara,K., Fujii,T., Nagahama,K., Omata,T.,
            Inoue,Y., Tanase,S. and Morino,Y.
  TITLE     Molecular cloning in Escherichia coli, expression, and nucleotide
            sequence of the gene for the ethylene-forming enzyme of Pseudomonas
            syringae pv. phaseolicola PK
  JOURNAL   Biochem. Biophys. Res. Commun. 188, 826-832 (1992)
  STANDARD  full automatic
COMMENT     Submitted (07-SEP-1992) to DDBJ by: Hideo Fukuda Applied Microbial
            Technology The Kumamoto Institute of Technology 4-22-1 Ikeda,
            Kumamoto Kumamoto 860 Japan Phone:  096-326-3111 x5134 Fax:
            096-326-3000.
FEATURES             Location/Qualifiers
     -35_signal      383..388
     -10_signal      407..412
     repeat_region   1644..1685
                     /rpt_type=inverted
                     /rpt_unit=1644..1662
                     /rpt_unit=1667..1685
     CDS             519..1571
                     /product="'Ethylene-forming enzyme'"
                     /codon_start=1
                     /translation="MTNLQTFELPTEVTGCAADISLGRALIQAWQKDGIFQIKTDSEQ
                     DRKTQEAMAASKQFCKEPLTFKSSCVSDLTYSGYVASGEEVTAGKPDFPEIFTVCKDL
                     SVGDQRVKAGWPCHGPVPWPNNTYQKSMKTFMEELGLAGERLLKLTALGFELPINTFT
                     DLTRDGWHHMRVLRFPPQTSTLSRGIGAHTDYGLLVIAAQDDVGGLYIRPPVEGEKRN
                     RNWLPGESSAGMFEHDEPWTFVTPTPGVWTVFPGDILQFMTGGQLLSTPHKVKLNTRE
                     RFACAYFHEPNFEASAYPLFEPSANERIHYGEHFTNMFMRCYPDRITTQRINKENRLA
                     HLEDLKKYSDTRATGS"
BASE COUNT      440 a    468 c    495 g    406 t
ORIGIN      
        1 gcgaccgcgt gtcagcaacg acaatcctta ctcggagtcg ctgttcagga cactgaagta
       61 ctgcccgcaa tggccgcagg atgggtttgc cagtcttgac gcggcacgct ctgggtgagg
      121 gatttcatgc gttggtacaa caatgatcac cggcacagcc gaatccgctt cgtgacaccg
      181 gctgagcggc atcgagggct ggatcatcag atcctggcca agcgacatga gctgtacgag
      241 ctagccagag agaaaaggcc ggagcggtgg tcgagggaga cacgcaactg ggaaccgatc
      301 ggcaccgtgc tgttaaaccc ggatcgagag caagacgttg agaaaaaagc agcatagtta
      361 gacggttgac gcgacaacta ccttgaaaaa cgccggcagg gacgctcatg attcatgctc
      421 caagcaggtg gggtgttttt ccgtcgcttg gactttgttc tcccgacgtc gtttggaatg
      481 agcatccgtc cctttatatg gataaagaag agactagcat gaccaaccta cagactttcg
      541 agttgcctac cgaggtaacc ggctgcgccg ccgatatctc attgggaagg gcgctgatcc
      601 aagcctggca aaaagatggc atttttcaga tcaagaccga tagtgagcag gatcgcaaaa
      661 cgcaggaagc aatggctgct agcaagcagt tttgcaagga accgctgact tttaagagta
      721 gctgcgttag cgatctgacc tacagcggct atgttgcgtc aggcgaggaa gtcacagctg
      781 gtaaacctga tttccctgaa atcttcactg tctgcaagga cttgtcggta ggcgatcagc
      841 gtgtaaaagc cggctggcct tgccatggtc cggtgccatg gccaaataac acctatcaga
      901 aaagcatgaa gaccttcatg gaagagctgg gtttagcggg cgaacggttg ctcaaactga
      961 cagcgctcgg ctttgaacta cccatcaaca cgttcaccga cttaactcgc gatggttggc
     1021 accacatgcg tgtattacgc ttcccgcccc aaacatccac gctgtcccgt ggaattggtg
     1081 cgcacactga ctatgggttg ttggtaattg ccgctcagga cgatgttggt ggcttatata
     1141 ttcgccctcc agtcgaggga gagaagcgta atcgtaactg gttgcctggt gagagctcag
     1201 caggcatgtt tgagcacgat gaaccttgga ccttcgtgac gcccacccca ggcgtgtgga
     1261 cagttttccc aggtgatatc ttgcagttca tgaccggcgg ccagctgctt tccactccgc
     1321 acaaggttaa gctcaatacc cgcgaacgtt tcgcctgcgc ttattttcat gagcctaatt
     1381 ttgaagcatc cgcctatccg ttgttcgagc ccagcgccaa tgagcgtatt cattatggtg
     1441 agcactttac caacatgttt atgcgttgct atccagatcg gatcaccacc cagaggatca
     1501 acaaggagaa tcgcctggcg cacttggagg acttgaagaa gtattcggac acccgcgcga
     1561 caggctcatg agtcgacacc ctgcccggtg ctgccggaca ggggccttat cgttactggt
     1621 gactaataat tggcatatca atgtccactc agcacccaga tcttatgatc tgggtgctga
     1681 gtggagcaat gtaaccatta tgctgagcgt tcatgcatag gaatttcaat aattcctata
     1741 caaggcaatc cgccgaaaaa ggtcccctcg gcgtgatgcc aacgtggcgt cgatgtcggc
     1801 aaaaagctt
//