From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: D.pseudoobscura Adh gene for alcohol dehydrogenase
Date: 28 Dec 92 21:27:08 GMT
Distribution: bionet
Checksum: 04635 8

LOCUS       DROADHGN38   3486 bp ds-DNA             INV       01-DEC-1992
DEFINITION  D.pseudoobscura Adh gene for alcohol dehydrogenase
ACCESSION   X62218
KEYWORDS    ADH gene; alcohol dehydrogenase.
SOURCE      Drosophila pseudoobscura DNA.
  ORGANISM  Drosophila pseudoobscura
            Eukaryota; Animalia; Metazoa; Arthropoda; Uniramia; Insecta;
            Pterygota; Neoptera; Holometabola; Diptera; Brachycera;
            Cyclorrhapha; Schizophora; Drosophiloidea; Drosophilidae.
REFERENCE   1  (bases 1 to 3486)
  AUTHORS   Schaeffer,S.W. and Miller,E.L.
  TITLE     Estimates of gene flow in Drosophila pseudoobscura determined from
            nucleotide sequence analysis of the alcohol dehydrogenase region
  JOURNAL   Genetics 132, 471-480 (1992)
  STANDARD  full automatic
COMMENT     *source: strain=MT228; *source: isolate=Tulancingo, Mexico;
            *source: chromosome=IV section 88;
               From EMBL    entry DPADHGN38;  dated 16-OCT-1992.
FEATURES             Location/Qualifiers
     mRNA            join(40..119,915..1020,1084..1488,1549..1964)
                     /note="adult mRNA (Adh)"
     mRNA            join(882..1020,1084..1488,1549..1964)
                     /note="larval mRNA (Adh)"
     promoter        8..14
                     /note="adult promoter (Adh)"
     prim_transcript 40..1964
                     /note="adult primary transcript (Adh)"
     exon            40..119
                     /note="adult exon 1a"
     intron          120..914
                     /note="adult intron"
     promoter        846..853
                     /note="larval promoter (Adh)"
     prim_transcript 882..1964
                     /note="larval primary transcript (Adh)"
     exon            882..1020
                     /note="larval exon 1"
     exon            915..1020
                     /note="adult exon 1b"
     intron          1021..1083
                     /number=1
     exon            1084..1488
                     /number=2
     intron          1489..1548
                     /number=2
     exon            1549..1964
                     /number=3
     polyA_signal    1941..1946
     polyA_site      1964
     CDS             join(928..1020,1084..1488,1549..1815)
                     /EC_number="1.1.1.1"
                     /gene="Adh"
                     /product="alcoholdehydrogenase"
                     /codon_start=1
                     /translation="MSLTNKNVVFVAGLGGIGLDTSRELVKRNLKNLVILDRIDNPAA
                     IAELKAINPKVTITFYPYDVTVPVAETTKLLKTIFAQVKTIDVLINGAGILDDHQIER
                     TIAVNYTGLVNTTTAILDFWDKRKGGPGGIICNIGSVTGFNAIYQVPVYSGSKAAVVN
                     FTSSLAKLAPITGVTAYTVNPGITKTTLVHKFNSWLDVEPRVAEKLLEHPTQTSQQCA
                     ENFVKAIELNKNGAIWKLDLGTLEPITWTQHWDSGI"
BASE COUNT      972 a    793 c    802 g    919 t
ORIGIN      
        1 cgtagtgtat ttaaggcact gcacatgtcg aggaccacga ttattgtcta cagtgcagtc
       61 agtagttgtc aggtgcagca ggcggaccag catttcttta aatctactca atcgatcaag
      121 taagtaactg taatccaagt acagtcggcc catcccactc ggttctgccg taactcaaga
      181 aatttcttga tagcgcagtt ccagttgtag aacgtcgttt aaaaaaacat ctcgttggag
      241 cttgggttga aaaacgttcg agttgggcgt agaaaatttc aaatataaaa tatcaaaaaa
      301 gagaaaatat cttcccaaag cagctgtcca ttttttgtta aagtgataga tcaaaaattc
      361 ggatgacgta cattcctagt gcacttcata caaataattt cctcaattct aattctacaa
      421 gaagcacttt tggcacttgt ccagaaaaaa caatttgttt acacttttcg gttgataaca
      481 gaggcggacc gacggctaag taacataaca gtcgcctgtt aagtaatgcc ctcactctct
      541 gttagatggc cgcgcgtgtc gactccgata gaccagcaga cacgcagtag ttttcctcaa
      601 gaacctgaac tctaaacata gacataattt acgatacctt gagactcaaa taaaaataca
      661 agtatgagtg gtattctatt cctgattaga ctcgcacaca cacacgtacg agtattaaca
      721 taaatttgcc ggcaaataac caaaacaaaa cgaacgcaaa cgatccgaac acgtaatgcg
      781 agagataaga aacagaaaag ctcgacgtga aagaagcttc tgacggaagc tcttgccgaa
      841 ctccctataa atacgagaca gaaaaccaga ataaatataa taacaaactg ttgaattgtc
      901 ccatcgattc ccagatcaaa agacagaatg tctctcacca acaagaacgt cgttttcgtg
      961 gccggtctgg gcggcattgg cctggacacc agccgggagt tggtcaagcg taatctgaag
     1021 gtaagagaga acgaattcca tggaatctat ggaatcctaa atttaaaaat ttatattttt
     1081 tagaacctgg tcatcctgga tcgcattgac aatccggctg ccattgccga actgaaggca
     1141 atcaatccca aggtgaccat caccttctat ccctacgatg tgactgtgcc cgtcgctgag
     1201 accaccaagc tcctgaagac catctttgcc caggtgaaga caatcgatgt cctgatcaac
     1261 ggtgctggca tcctggacga tcatcagatc gagcgcacca ttgccgttaa ctacacgggc
     1321 ctggtcaaca ccaccacagc cattctggac ttctgggaca agcgcaaggg cggcccaggc
     1381 ggcatcattt gcaacattgg ctccgtcacc ggtttcaatg ccatctacca ggtgcccgtt
     1441 tactccggct ccaaggcggc ggtggttaac tttacctcct ccctggcggt aagtaatcca
     1501 acgtttttcc tactccccaa aactaaatat ttctttgaaa ttccatagaa actggctccc
     1561 attactggtg tcactgctta cactgtcaat cctggcatca cgaagaccac tctggtccac
     1621 aaattcaact cgtggctgga tgtggagccc cgtgtggcgg agaagctgct cgagcatccc
     1681 acccagacct ctcagcagtg cgccgagaac tttgtgaagg ccattgagct gaacaagaac
     1741 ggtgccatct ggaagttgga tctgggcacc ttggagccca tcacatggac ccagcactgg
     1801 gactcgggca tctaaaccat cccagagact ctatgggaca tggcgtttta gcttttagtt
     1861 tctttttttc cactcaattg ttacgtatat acatacacat atggcaataa ggctgatttg
     1921 aaccccgttt tgaatatgat aatacaaaat tatatttgag aaatttcaac aaaatcgata
     1981 agagctatag ctctctttta attaaaaaaa actagataaa aagagcaatg gtcagtggtg
     2041 gtcagtggtg gtcagtggtc gttgtggcct ctatcgattt ccacacaaaa actttactgt
     2101 ttagtaatag aaaagaactt gagaaaggca gccaaaagaa tgtacgatct gacgggtaag
     2161 catgtctgct atgtggctga ctgcggtggc attgcactgg agaccagcaa ggttctcatg
     2221 accaagaata tagcggtgag tggtagagtg gtgtagagag tggagagtgt tccgaagtgg
     2281 ttctggttct taaacaagtc tctagtctct agtctcttgg ctgctgctgg tagctgtcga
     2341 aactaattaa gttaatgact ttttcatgtt ttgtttttgt ccgctgactt ttgctgttca
     2401 aagctacaat taagtttatg ttttgagttt gatctacttt tgcttaaggg aagtactttc
     2461 gattacggat tgctggcata taaaaataga taccaaggaa tgtgcatcat atcatattga
     2521 ttctatcttg attctttgcc cagaaactgg ccatccttca gagcgtggaa aaccccccgg
     2581 ccatcgccca gttacaatcg attaagcaca gcacacagat cttcttctgg accttcgatg
     2641 tgaccatggc ccgcgaggag atgaagaagt acttcgatga ggtcatggtc cagatggact
     2701 acatcgatgt gctgatcaat ggggccaccc tgtgcgatga gcggaacatt gatgccacca
     2761 tcaatacgaa tctgaccggc atgatgaaca ccgtggccac tgtgctgccc tatatggacc
     2821 gaaagatggg cggttcgggt ggactgattg tgaatgtcac ctccgtcata ggactggatc
     2881 catcgccggt cttttgtgcg tacagtgcct caaagtttgg tgtgattggg ttcacccgaa
     2941 gtctagcggt gagtgggcga gatcttagct tgaaaatgct ctctaatgtc tttcattctt
     3001 tcgcaggatc ccctgtattt cacccaaaat ggtgtggctg tcatggccgt ctgctgtggt
     3061 cccaccaaag tgtttgtcga tcgggaactg actgccttcc tggcatacgg ccagtccttt
     3121 gccgatcgcc tgcgtcgtgc cccctgccaa tcgacggccg tctgcggcca gaatatagta
     3181 aatgccatcg agagatcgga gaacggacag atctggattg ccgacaaggg cggcctggag
     3241 tcggtggccc tccactggta ttggcatatg gccgatcagt ttgtgaacta tatgcagagc
     3301 actgacgatg aagatcagga gttttttttg ggccagcgat gaggcatgcc ttgatccacg
     3361 gaataaaaca tcaaatgtgt cacaaaaagt aggcaacttg tttgtgtgtt tggaaataga
     3421 tttgaaatat tttgaggtca tatgaataat gacataaatg agggtgcctg aaagtatgcc
     3481 aagttt
//