From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: S.coelicolor glk gene for glucose kinase
Date: 24 Jul 92 10:10:12 GMT
Distribution: bionet
Checksum: 39563     7

LOCUS       STMGLKG      2804 bp ds-DNA             BCT       24-JUL-1992
DEFINITION  S.coelicolor glk gene for glucose kinase
ACCESSION   X65932
KEYWORDS    glk gene; glucose kinase.
SOURCE      Streptomyces coelicolor DNA.
  ORGANISM  Streptomyces coelicolor
            Prokaryotae; Firmicutes; Streptomycetaceae.
REFERENCE   1  (bases 1 to 2804)
  AUTHORS   Angell,S., Schwarz,E. and Bibb,M.J.
  TITLE     The glucose kinase gene of Streptomyces coelicolor A3(2): its
            nucleotide sequence, transcriptional analysis and role in glucose
            repression
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
COMMENT     *source: strain=a3(2);
            From EMBL    entry SCGLKG;  dated 13-MAY-1992.
FEATURES             Location/Qualifiers
     CDS             <1..1028
                     /gene="ORF1"
                     /codon_start=3
                     /translation="RTGPAPTSVEPGLTAWRPDAAQGFRDALAALQDRAASALDLLGA
                     ARLDPQELTPLPGADDLALLRALREAALAEAHDLLVVDLPPAPRALALLAAPEELRRA
                     LRRLLPPERQAARALRPVLGRLAGVPMPTEALYEAAARWDLELAAAESVLADRNTVVR
                     LVAEPGPAGADAVRTTTLGLALRGLRTDLLVANRVLPEDTPADSWLSGPLAQQRKTLE
                     EWRGAYDVRAVAHLGHDPRGTDDLAALAVPGVNPDASPVEWPVTDRLAEDGVLVWHIP
                     LPGAVREELDLVRRGDELAVAAGPFRRTVPLPSALRRCTVDGAALRDGTLAVRFAPDP
                     ELWPRGR"
     prim_transcript 1074..>2682
                     /note="transcript for ORF2 & ORF3"
     RBS             1088..1092
                     /gene="ORF2"
     CDS             1099..1674
                     /gene="ORF2"
                     /codon_start=1
                     /translation="MSEELPPSEAPRPDEADAVDETRATGETRGAAQEPGASDADAWA
                     TACAEDLEAEKARRRAAYGPPPGSAAEELRRLVDTVADKLSGLQSPLLGQVAGPAAQQ
                     VVRQVVQQAKAAVEPVIERNPDLFDHLAAAGGELLAAYRSAVQNQERRWTTGDTAPKD
                     PSDPRDLDERGTDGRDEGDGGTGPGQRIDLD"
     prim_transcript 1701..>2682
                     /gene="glk"
     RBS             1720..1723
                     /gene="glk"
     CDS             1729..2682
                     /product="glucose kinase"
                     /gene="glk"
                     /codon_start=1
                     /translation="MGLTIGVDIGGTKIAAGVVDEEGNILSTHKVPTPTTPEAIVDAI
                     ASAVEGARVGHEIVAVGIGAAGYVNRQRSTVYFAPNIDWRQEPLKEKVEARVGLPVVV
                     ENDANAAAWGEYKFGGGKGHRNVICITLGTGLGGGIIIGNKLRRGHFGVAAEFGHIRM
                     VPDGLLCGCGSQGCWEQYASGRALVRYAKQRANATPERAEVLLALGDGTPDGIEGKHI
                     SVAARQGCPVAVDSYRELARWAGAGLADLASLFDPSAFIVGGGLSDEGDLVLDPIRKS
                     YKRWLVGGNWRPVADVIAAQLGNKAGLVGAADLAREPDPIM"
BASE COUNT      381 a   1150 c    961 g    312 t
ORIGIN
        1 tgcgcaccgg cccggccccg acctccgtcg aacccggcct caccgcttgg cgccccgacg
       61 ccgcccaggg cttccgggac gccctcgccg ccctccagga ccgcgccgcc tccgcgctcg
      121 acctcctcgg cgccgcccgc ctcgaccccc aggaactcac cccgctgccc ggggccgacg
      181 acctcgccct gctgcgtgct ctgcgcgagg ccgccctcgc cgaggcgcac gacctcctcg
      241 tcgtcgacct gccgcccgcc ccgcgcgccc tcgccctgct cgccgccccc gaggaactgc
      301 gccgcgccct gcgccgcctg ctcccgcccg agcggcaggc cgcccgcgcc ctgcgcccgg
      361 tcctcggccg gctcgccggc gtccccatgc cgaccgaggc gctgtacgag gccgccgccc
      421 gctgggacct ggaactcgcc gccgccgaat ccgtcctggc cgaccggaac accgtcgtac
      481 ggctggtcgc cgagcccgga cccgccggag ccgacgccgt ccgcaccacc accctcggtc
      541 tggccctgcg cggactgcgc accgacctgc tcgtcgccaa ccgcgtgctg cccgaggaca
      601 ccccggcgga cagctggctc agcggccccc tcgcccagca gcgcaagacc ctggaggagt
      661 ggcggggggc gtacgacgtc cgcgccgtgg cccatctcgg ccacgacccg cgcggcacgg
      721 acgacctcgc ggccctcgcc gtgccgggcg tcaacccgga cgcctccccg gtcgagtggc
      781 ccgtcaccga ccggctggcc gaggacggcg tgctggtctg gcacatcccg ctgcccggcg
      841 ccgtacgcga ggagctcgac ctggtccgcc gcggcgacga actggccgtc gccgccgggc
      901 cgttccgccg caccgtcccg ctgccgtcgg cgctgcgccg ctgcaccgtc gacggggccg
      961 ccctgcgcga cggcacgctc gccgtccggt tcgcgcccga cccggaactg tggccgcggg
     1021 ggcgatgacc cgcggagccc cggagcgcgg gcccgttcgg gtaacgtcgt aaggacgaac
     1081 cgtagtcagg agtccgtcat gagcgaagag ctccccccgt ccgaggcccc ccgccccgac
     1141 gaggccgacg cggtcgacga gacgcgagcg accggcgaga cgcgcggggc ggcgcaggag
     1201 ccgggtgcga gcgacgccga cgcctgggcg accgcgtgcg ccgaggacct ggaggccgag
     1261 aaggcccgcc gccgggccgc gtacgggccg ccgcccggct cggccgccga ggagctgcgc
     1321 cggctcgtcg acaccgtcgc ggacaagctg tccggtctcc agtcccccct gctcggccag
     1381 gttgccggac ccgccgccca gcaggtggtc cggcaggtcg tccagcaggc caaggccgcc
     1441 gtcgaaccgg tcatcgagcg caacccggac ctgttcgacc acctcgccgc cgccggtggt
     1501 gaactgctcg ccgcctaccg ctccgccgtc cagaaccagg aacgccgctg gaccaccggg
     1561 gacaccgcgc ccaaggaccc gagcgaccca cgtgacctgg acgaacgcgg caccgacggc
     1621 cgcgacgagg gcgacggagg caccggcccg gggcagcgca tcgacctgga ctgagtcctg
     1681 cctcgggtac ggttgccctt agcggggctc gaccgaactg agggattcat gggactcacc
     1741 atcggcgtcg acatcggcgg cacgaagatc gcggccggcg tggtcgacga ggaaggcaac
     1801 atcctctcga cccacaaggt gccgaccccc accacacccg aggccatcgt ggacgccatc
     1861 gcctccgcgg tcgagggggc gcgagtgggg cacgagatcg tcgcagtggg catcggcgcc
     1921 gccggatacg tcaaccgcca gcgctccacg gtctacttcg cgcccaacat cgactggcgc
     1981 caggagccgc tcaaggagaa ggtcgaggcc cgcgtcggcc tcccggtcgt cgtggagaac
     2041 gacgccaacg ccgcggcctg gggcgagtac aagttcggcg gcggcaaggg ccaccgcaac
     2101 gtcatctgca tcaccctcgg caccggcctc ggcggcggca tcatcatcgg caacaagctg
     2161 cgccgcggcc acttcggcgt ggccgcggag ttcggccaca tccgcatggt gccggacggc
     2221 ctgctgtgcg gctgcggctc gcagggctgc tgggagcagt acgcgtcggg gcgggcgctg
     2281 gtgaggtacg ccaagcagcg cgccaacgcc acccccgagc gcgccgaggt gctgctcgcc
     2341 ctcggcgacg gcacccccga cggcatcgag ggcaagcaca tctcggtcgc cgcccgccag
     2401 ggctgcccgg tcgccgtcga ctcctaccgg gagctggccc gctgggccgg ggccggtctc
     2461 gccgacctgg cctcgctctt cgacccgtcc gccttcatcg tcggcggcgg cctctcggac
     2521 gagggcgacc tggtcctcga cccgatccgc aagtcctaca agcgctggct ggtcggcggc
     2581 aactggcgcc cggtcgccga cgtgatcgcc gcccaactcg gcaacaaggc cggcctggtc
     2641 ggcgccgccg acctggcgag agaacccgac ccgatcatgt gacgatcact tcccgactcc
     2701 gggagctgcg ctcacccgag gggcgcgggg aactgcgcga ccagccacga cgaaccggca
     2761 gtcgcccagg aaccccgcgc cccgacccat gaggcgcctg atca
//