From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Clostridium thermocellum (clone RV 1.6) endo-1, 3-beta-glucanase
Date: 6 Nov 92 18:05:45 GMT
Distribution: bionet
Checksum: 08095 7

LOCUS       CLOFEEGASE   3048 bp ds-DNA             BCT       28-OCT-1992
DEFINITION  Clostridium thermocellum (clone RV 1.6) endo-1, 3-beta-glucanase
            (CMC) gene, complete cds.
ACCESSION   L04735
KEYWORDS    cellulase; endo-beta-1,3-glucanase.
SOURCE      Clostridium thermocellum (library: pBR322; NCIB 10682) DNA.
  ORGANISM  Clostridium thermocellum
            Prokaryotae; Firmicutes; Endospore-forming rods and cocci;
            Bacillaceae.
REFERENCE   1  (bases 1 to 3048)
  AUTHORS   Hazlewood,G.P., Davidson,K., Laurie,J.I., Huskisson,N.S. and
            Gilbert,H.J.
  TITLE     Gene Sequence and Properties of CelI, a Family E Endoglucanase from
            C.thermocellum
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     RBS             245..251
                     /gene="CMC"
                     /note="putative"
                     /citation=[1]
     sig_peptide     256..417
                     /gene="CMC"
                     /evidence="EXPERIMENTAL"
                     /codon_start=1
                     /citation=[1]
     mat_peptide     418..2892
                     /product="endo-1,3-beta-glucanase"
                     /gene="CMC"
                     /codon_start=1
     CDS             256..2895
                     /function="cellulase"
                     /EC_number="3.2.1.4"
                     /evidence="EXPERIMENTAL"
                     /citation=[1]
                     /gene="CMC"
                     /product="endo-1,3-beta-glucanase"
                     /codon_start=1
                     /translation="MRLVNSLGRRKILLILAVIVAFSTVLLFAKLWGRKTSSTLDEVG
                     SKTHGDLTAENKNGGYLPEEEIPDQPPATGAFNYGEALQKAIFFYECQRSGKLDPSTL
                     RLNWRGDSGLDDGKDAGIDLTGGWYDAGDHVKFNLPMSYSAAMLGWAVYEYEDAFKQS
                     GQYNHILNNIKWACDYFIKCHPEKDVYYYQVGDGHADHAWWGPAEVMPMERPSYKVDR
                     SSPGSTVVAETSAALAIASIIFKKVDGEYSKECLKHAKELFEFADTTKSDDGYTAANG
                     FYNSWSGFYDELSWAAVWLYLATNDSSYLDKAESYSDKWGYEPQTNIPKYKWAQCWDD
                     VTYGTYLLLARIKNDNGKYKEAIERHLDWWTTGYNGERITYTPKGLAWLDQWGSLRYA
                     TTTAFLACVYSDWENGDKEKAKTYLEFARSQADYALGSTGRSFVVGFGENPPKRPHHR
                     TAHGSWADSQMEPPEHRHVLYGALVGGPDSTDNYTDDISNYTCNEVACDYNAGFVGLL
                     AKMYKLYGGSPDPKFNGIEEVPEDEIFVEAGVNASGNNFIEIKAIVNNKSGWPARVCE
                     NLSFRYFINIEEIVNAGKSASDLQVSSSYNQGAKLSDVKHYKDNIYYVEVDLSGTKIY
                     PGGQSAYKKEVQFRISAPEGTVFNPENDYSYQGLSAGTVVKSEYIPVYDAGVLVFGRE
                     PGSASKSTSKDNGLSKATPTVKTESQPTAKHTQNPASDFKTPANQNSVKKDQGIKGEV
                     VLQYANGNAGATSNSINPRFKIINNGTKAINLSDVKIRYYYTKEGGASQNFWCDWSSA
                     GNSNVTGNFFNLSSPKEGADTCLEVGFGSGAGTLDPGGSVEVQIRFSKEDWSNYNQSN
                     DYSFKQACLRQRTLIYLYATWLR"
BASE COUNT      957 a    529 c    757 g    805 t
ORIGIN      
        1 ggtaaatact aggaaaacgt aaaataaaaa tacgtaaagc agagtgaatt ttgacattta
       61 ctctgctttt ttaaaatacc aaaaaaggca tgtccgaaaa atttttgaaa tctatacggc
      121 aatttttctg aaatttggtt ttatcaaatt tgacgaagca tatatgatat aataaactaa
      181 taaagcggat aaatatagat ttacttaaaa ttaaagttgt aaattgattt ttattaattg
      241 aggcaggagg atgctatgag gctggttaac agtttgggaa gaagaaaaat tcttttgata
      301 cttgctgtta ttgtagcttt cagcactgtt ctgttgtttg caaagctatg ggggcgaaag
      361 acttcaagta ctttggatga ggttggttca aaaactcatg gtgatttgac ggcagaaaat
      421 aaaaacggcg gatatttacc agaggaagag attccagatc agcctccggc aaccggggcc
      481 ttcaactacg gtgaagcgtt gcaaaaagca atttttttct atgagtgtca aagatccgga
      541 aagctcgatc cctcaactct tcgcctaaat tggcggggag attcgggact ggatgacgga
      601 aaagatgcag gaattgatct taccggcgga tggtatgatg cgggagatca cgtaaaattt
      661 aatttgccca tgtcttattc ggcggctatg ctggggtggg cggtgtatga atatgaagat
      721 gcgtttaaac agagtggaca gtataaccac atattgaata acataaaatg ggcttgtgat
      781 tattttataa aatgtcatcc ggaaaaggat gtgtactatt accaggtggg cgacggtcat
      841 gctgaccatg cgtggtgggg tcctgccgaa gtaatgccta tggaaaggcc gtcgtacaaa
      901 gttgacaggt catcgccggg ttccacggtt gtggcagaga cgtcggcagc tttagctata
      961 gcatcgataa tatttaagaa agtggatggt gaatactcga aagaatgttt aaagcatgca
     1021 aaagaactgt ttgaatttgc ggacaccaca aaaagcgatg atgggtacac tgcagccaat
     1081 ggtttttata attcatggag cggattttat gatgagcttt cctgggcagc tgtatggctt
     1141 tatcttgcta ccaatgattc ttcatatttg gataaagcgg aaagttattc tgacaaatgg
     1201 ggttatgagc cacagacgaa cataccgaag tataagtggg ctcaatgctg ggatgatgtg
     1261 acttatggca cttatcttct tttggccagg attaaaaatg acaacggaaa atataaagaa
     1321 gcgatagaaa ggcatcttga ttggtggaca accggataca acggtgaaag aattacatat
     1381 actccgaagg gacttgcatg gctcgaccag tggggatcat tgaggtatgc aaccacaacg
     1441 gcatttctgg catgtgttta ttccgattgg gagaacggtg ataaggaaaa agcaaaaact
     1501 tatctggagt ttgcaagaag ccaggcggat tatgctttgg gaagcacggg aagaagcttt
     1561 gttgtgggtt ttggagaaaa tccaccgaaa aggccccatc acagaactgc tcacggttca
     1621 tgggcggaca gtcagatgga gcctcccgaa cacaggcatg ttctttatgg tgcccttgtg
     1681 ggaggacctg acagcacgga caactacacc gacgacatca gtaattacac ctgcaatgaa
     1741 gttgcctgtg actataatgc aggttttgtg ggactgcttg caaaaatgta caagctttat
     1801 ggcggaagtc ccgatcccaa atttaacggt atagaagaag ttccggagga tgaaatattc
     1861 gttgaagccg gtgtgaatgc atcgggaaac aatttcattg aaataaaagc gatagttaat
     1921 aataaatcgg gctggcctgc aagagtatgt gagaatttat cctttagata ttttatcaac
     1981 attgaagaga ttgtgaatgc gggaaaaagt gcaagcgacc tgcaagtgag ctccagctac
     2041 aatcaggggg caaaactgtc cgatgtaaag cactacaagg acaatattta ttatgtggaa
     2101 gtggatttgt cggggacaaa aatatatccc gggggacaat cggcatacaa gaaggaagtg
     2161 cagtttagaa tttccgcgcc ggagggcacg gtgtttaatc cggaaaacga ctattcctat
     2221 cagggacttt cggcaggtac ggttgtaaag tctgagtata ttccggtata tgatgccggg
     2281 gtgctggtat ttggaaggga accgggctca gcatcgaaaa gcacgtctaa agacaatggt
     2341 ttgtccaagg caactcccac ggtgaaaact gaatctcagc cgacagcaaa acacactcaa
     2401 aatcctgcct cagactttaa aactccagcc aatcagaaca gtgtaaaaaa agaccaaggc
     2461 ataaaaggag aagtggtatt acagtacgca aacgggaatg caggtgctac gtcaaacagt
     2521 attaatccga ggtttaaaat aattaacaac ggtacaaaag ccataaattt gtccgatgtc
     2581 aagattagat attattacac aaaagaaggg ggcgcatctc aaaacttctg gtgtgattgg
     2641 agcagtgccg gcaattcaaa tgttacagga aacttcttta atctttcttc accgaaagaa
     2701 ggagcggaca cctgtcttga agttggtttc ggaagtgggg ccggaaccct tgatcctggt
     2761 ggaagcgttg aagtacagat aaggttttca aaggaagact ggtcaaacta taaccagtca
     2821 aacgattatt ctttcaaaca agcttgttta cggcaaagaa ccttgattta tctgtacgca
     2881 acatggctaa gataggcttt tgcaataccg atgtaatatt cggattctct tacaatgtga
     2941 ttaattacaa cagaggctgt caaattgttt gccactgcgg cactctcttt tgccatgcag
     3001 tttaggaagt ttacgaaggt ttgactttga cgtagtgtta tttcgaat
//