From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Schizosaccharomyces pombe adenylsuccinate synthetase (ade2) mRNA,
Date: 21 Jan 93 05:50:36 GMT
Distribution: bionet
Checksum: 61677 5

LOCUS       YSPADE2      1915 bp ss-mRNA            PLN       20-JAN-1993
DEFINITION  Schizosaccharomyces pombe adenylsuccinate synthetase (ade2) mRNA,
            complete cds.
ACCESSION   M98805
KEYWORDS    ade2 gene; adenylosuccinate synthetase.
SOURCE      Schizosaccharomyces pombe (strain Sp 223) cDNA to mRNA.
  ORGANISM  Schizosaccharomyces pombe
            Eukaryota; Fungi; Eumycota; Hemiascomycetes; Endomycetales;
            Schizosaccharomycetoideae.
REFERENCE   1  (bases 1 to 1915)
  AUTHORS   Speiser,D.M., Ortiz,D.F., Kreppel,L., Scheel,G., McDonald,G. and
            Ow,D.W.
  TITLE     Purine biosynthetic genes are required for Cadmium tolerance in
            Schizosaccharomyces pombe
  JOURNAL   Mol. Cell. Biol. 12, 5301-5310 (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     exon            42..84
     intron          85..123
     exon            124..222
     intron          226..604
     exon            606..1768
     CDS             join(42..84,124..222,606..1768)
                     /note="putative"
                     /product="adenylosuccinate synthetase"
                     /codon_start=1
                     /translation="MASVRETGVNVSNDGITVVLGSQWGDEGKGKLVDILCDNVDVCA
                     RCQGGNNAGHTIVANGVTYDFHILPSGLVNPKCQNLIGSGVVVYLPAFFSELEKLVQK
                     GLKCRDRLFISDRAHLVFDYHQRADALNEAELGKQSIGTTGKGIGPAYSTKATRSGIR
                     VHHLYHWAEFEARYRKNVADLQKRYGAFEYDVEAELIRYKELAQRLKPFVIDAVAFMY
                     EALQSKKRILVEGANALMLDLDFGTYPFVTSSNTTVGGVCTGLGVPPQRIANSIGVVK
                     AYTTRVGAGPFPTEQLNEIGDHLQSVGREVGVTTGRKRRCGWLDLVVVKYSTMINGYT
                     SLNLTKLDILDALDEIKVAVAYIINGKRIETFPADLDSLEEAEIVYETFPGWKVPTTG
                     ITHWDQMPENAKKYIEFIEKFVGVPITFIGVGPGRDEMLVKE"
BASE COUNT      519 a    344 c    393 g    659 t
ORIGIN      
        1 atcaccaaaa cggagatcta ataaaaacat agaagaaaaa tatggcttca gtgcgagaaa
       61 ctggtgtaaa tgtttccaat gatgggtaag aaaattcttt gagaagaatt tactattcac
      121 acagaataac cgtcgtcctt ggcagtcaat ggggtgacga aggaaagggt aaacttgttg
      181 atatcctttg cgataatgtt gatgtctgtg ctcgctgtca ggtatgttac aaaagcttta
      241 tatagtggtg aaccgaacga ggtgatattg tgtaaaattt tttttcactc tactttgact
      301 cttaagtgcg ggacgtcgcg gaattactgc cgatgaaaaa taacattttt aataaaaaat
      361 ttaatttttt gtttaaatgt taatacaaat ctgctaaatg acttttccta cttggtacag
      421 tcaagaaaag tattttagag ctcactggat aagacgatct gcatcagtaa caaataaatt
      481 tataccaatt ctgaatggtt tcttcttttt gatttaatta tggaggttac ctttatgatc
      541 ttgccagctt ttttgtttgc ccttgtattg atttttcaaa tatatagcta acttttattt
      601 ttagggtggt aacaatgctg gtcataccat tgttgctaat ggtgtcactt acgattttca
      661 cattcttcct tcaggactcg taaatcctaa atgccagaac ttgattggtt ctggtgttgt
      721 cgtctattta cctgcttttt ttagtgagtt agagaaactt gtgcaaaagg gtttgaaatg
      781 tagagatcgt cttttcattt ctgatcgcgc tcatctagta tttgactacc atcaacgtgc
      841 agatgcttta aacgaggcag agctcggaaa acaaagcatc ggaacaaccg gaaagggtat
      901 tggtcctgct tattccacca aggctactcg cagcggtatt cgtgttcatc acttgtatca
      961 ttgggctgaa tttgaagctc gttaccgtaa gaacgttgct gacttgcaga aacgttatgg
     1021 tgctttcgag tatgatgttg aagctgaact cattcgttat aaggaattgg ctcaaagact
     1081 taagccattt gtcattgatg ctgttgcgtt catgtatgaa gctttacaaa gcaagaaacg
     1141 tatccttgtc gaaggtgcta acgctttgat gcttgatttg gactttggaa cttatccatt
     1201 cgttaccagc tctaacacta ctgttggtgg tgtttgcact ggtttgggtg ttcctcctca
     1261 gcgcattgct aactccatcg gtgtagtgaa agcttataca actcgtgtag gcgctggtcc
     1321 tttcccaacg gaacagctca acgaaattgg tgaccatctt caaagtgttg gaagagaagt
     1381 tggtgttacc accggtcgta aacgccgctg cggctggctt gatttggtcg ttgtcaaata
     1441 ctctactatg atcaatggtt acacttcttt gaaccttacc aagcttgaca ttttggatgc
     1501 cttggatgag atcaaagttg ctgttgccta cattattaac ggtaaacgca ttgaaacttt
     1561 tcctgctgat cttgattctc ttgaagaagc cgagattgta tacgaaactt tccctggttg
     1621 gaaagttcct acaacaggta tcactcattg ggatcagatg cctgagaatg ccaagaaata
     1681 catagagttc attgaaaagt ttgttggtgt tcccatcact ttcatcggtg ttggtcctgg
     1741 tcgtgatgag atgttggtta aggagtaagt tacgatttac aactagatat cctcaccatt
     1801 cttcactact ttctttatct aaattttgtt tattatacat cttattttag ctcttttcca
     1861 tttattttta tagtgctttt ctttttttcg aatagattca taaagcatat aactc
//