From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Bacillus subtilis ilvB, ilvN and ilvC genes, complete ilv-leu
Date: 29 Sep 92 10:02:35 GMT
Distribution: bionet
Checksum: 20578     9

LOCUS       BACILNB      4281 bp ds-DNA             BCT       29-SEP-1992
DEFINITION  Bacillus subtilis ilvB, ilvN and ilvC genes, complete ilv-leu
            operon.
ACCESSION   L03181
KEYWORDS    attenuator; ilv-leu operon; ilvB gene; ilvC gene; ilvN gene;
            promoter region; ribosome binding site; transcription control.
SOURCE      Bacillus subtilis DNA.
  ORGANISM  Bacillus subtilis
            Prokaryotae; Firmicutes; Endospore-forming rods and cocci;
            Bacillaceae.
REFERENCE   1  (bases 1 to 4281)
  AUTHORS   Vandeyar,M.A., Vander Horn,P.B., Rafael,J.A., Grandoni,J.A. and
            Zahler,S.A.
  TITLE     The ilv-leu operon of Bacillus subtilis: Sequences of the ilvB,
            ilvN and ilvC genes, and the control of transcription
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     -35_signal      207..212
                     /gene="ilv-leu operon"
     -10_signal      230..235
                     /gene="ilv-leu operon"
     misc_feature    558..575
                     /standard_name="T-box"
                     /gene="ilv-leu operon"
     attenuator      578..650
                     /gene="ilv-leu operon"
     RBS             711..715
                     /gene="ilvB"
     CDS             723..2441
                     /product="acetolactate synthase"
                     /gene="ilvB"
                     /codon_start=1
                     /translation="MGTNVQVDSASAECTQTMSGRLMLIESLKKEKVEMIFGYPGGAV
                     LPIYDKLYIQVGTYPSRHEQGAIHAAEGYARVSGNRCRHCHVRPGATNLVTGLADAMI
                     DSLPLVVFTGQVATSVIGSDAFQEADILGITMPVTKHSYQVRQPEDLPRIIKEAFHIA
                     TTGRPGPVLIDIPKDVATIEGEFSYDHEMNLPGYQPTTEPNYLQIRKLVEAVSSAKKP
                     VILAGAGVLHGKASEELKNYAEQQQIPVAHTLLGLGGFPADHPLFLGMAGMHGTYTAN
                     MALHECDLLISIGARFDDRVTGNLKHFARNAKIAHIDIDPAEIGKIMKTQIPVVGDSK
                     IVLQELIKQDGKQSDSSEWKKQLAEWKEEYPLWYVDNEEEGFKPQKLIEYIHQFTKGE
                     AIVATDVGQHQMWSAQFYPFQKADKWVTSGGLGTMGFGLPAAIGAQLAEKDATVVAVV
                     GDGGFQMTLQELDVIRELNLPVKVVILNNACLGMVRQWQEIFYEERYSESKFASQPDF
                     VKLSEAYGIKGIRISSEAEAKEKLEEALTSREPVVIDVRVASEEKVFPMVAPGKGLHE
                     MVGVKP"
     RBS             2426..2430
                     /gene="ilvN"
     CDS             2438..2962
                     /product="acetolactate synthase small subunit"
                     /gene="ilvN"
                     /codon_start=1
                     /translation="MKRIITLTVVNRSGVLNRITGLFTKRHYNIESITVGHTETAGVS
                     RITFVVHVEGENDVEQLTKQLNKQIDVLKVTDITNQSIVQRELALIKVVSAPSTRTEI
                     NGIIEPFRASVVDVSRDSIVVQVTGESNKIEALIELLKPYGIKEIARTGTTAFARGTS
                     KRRHPIKQYLLYKT"
     RBS             2967..2971
                     /gene="ilvC"
     CDS             2979..4007
                     /product="ketol-acid reductoisomerase"
                     /gene="ilvC"
                     /codon_start=1
                     /translation="MVKVYYNGDIKENVLAGKTVAVIGYGSQGHAHALNLKESGVDVI
                     VGVRQGKSFTQAQEDGHKVFSVKEAAAQAEIIMVLLPDEQQQKVYEAEIKDELTAGKS
                     LVFAHGFNVHFHQIVPPADVDVFLVAPKGPGHLVRRTYEQGAGVPALFAIYQDVTGEA
                     RDKALAYAKGIGGARAGVLETTFKEETETDLFGEQAVLCGGLSALVKAGFETLTEAGY
                     QPELAYFECLHELKLIVDLMYEEGLAGMRYSISDTAQWGDFVSGPRVVDAKVKESMKE
                     VLKDIQNGTFAKEWIVENQVNRPRFNAINASENEHQIEVVGRKLREMMPFVKQGKKKE
                     AVVSVAQN"
BASE COUNT     1385 a    845 c   1046 g   1005 t
ORIGIN
        1 tcaggactca atccctatac gaaccatatc gtgttataaa atcttccatg tttatataaa
       61 attaaataat tctggtgtat tcttgataga ttaataaaaa aattataaaa acttttaaca
      121 tttgcaattc cttttgtacc aataatgaaa gcgtatacaa tatagattga ttaatcaaaa
      181 ttgtctaata attttaaaaa atgctgttga cactgcgtcc aaagcggcgt aatatgagtt
      241 caacaaaaga taaatgcaag cttcacaagc gaaaatcatc gcagtatgat tctaaaaaaa
      301 tgaaaaacaa acgaccttct tgaacagctg gaaagccgtt ccgaaggctg aatatgaaaa
      361 gcgcagatga ggataagtag ccttgataaa gttttccaca gagaaccggg ttagctgaga
      421 accggcgaag ctttacaagg tgaactcgcc tcagagtgcc agtctgaaat gacagtagga
      481 cttggccggg tgaacttgat tcactcgtta ctaaagcgga tagaaatatc catgagacgg
      541 ccgattaaca ggccgtaaac aagggtggta ccgcggaaag aaaagccttt tcgccccttt
      601 tagctatcgc agttactgcg cggctgattg tgggcggaag ggcttttttt attgaataat
      661 cagctatcta gctaatgaaa agatgatctt taaaggatga aaatccaaaa ggaggaacta
      721 aaatggggac taatgtacag gtggattcag catctgccga atgtacacag acgatgagcg
      781 gacgattaat gctgattgaa tcattaaaaa aagagaaagt agaaatgatc ttcggttatc
      841 cgggcggggc tgtgcttccg atttacgata agctatacat tcaggttggt acatatcctt
      901 cccgtcacga acaaggagca attcatgcag cggagggata cgcaagggtc tccggaaacc
      961 ggtgtcgtca ttgccacgtc aggccgggag cgacaaacct tgttacaggc cttgctgatg
     1021 ccatgattga ttcattgccg ttagtcgtct ttacagggca ggtagcaacc tctgtaatcg
     1081 ggagcgatgc atttcaggaa gcagacattt tagggattac gatgccagta acaaaacaca
     1141 gctaccaggt tcgccagccg gaagatctgc cgcgcatcat taaagaagcg ttccatattg
     1201 caacaactgg aagacccgga cctgtattga ttgatattcc gaaagatgta gcaacaattg
     1261 aaggagaatt cagctacgat catgagatga atctcccggg ataccagccg acaacagagc
     1321 cgaattattt gcagatccgc aagcttgtgg aagccgtgag cagtgcgaaa aaaccggtga
     1381 tcctggcggg tgcgggcgta ctgcacggaa aagcgtcaga agaattaaaa aattatgctg
     1441 aacagcagca aatccctgtg gcacacaccc ttttggggct cggaggcttc ccggctgacc
     1501 atccgctttt cctagggatg gcgggaatgc acggtactta tacagccaat atggcccttc
     1561 atgaatgtga tctattaatc agtatcggcg cccgttttga tgaccgtgtc acaggaaacc
     1621 tgaaacactt tgccagaaac gcaaagatag cccacatcga tattgatcca gctgaaatcg
     1681 gaaaaatcat gaaaacacag attcctgtag tcggagacag caaaattgtc ctgcaggagc
     1741 tgatcaaaca agacggcaaa caaagcgatt caagcgaatg gaaaaaacag ctcgcagaat
     1801 ggaaagaaga gtatccgctc tggtatgtag ataatgaaga agaaggtttt aaacctcaga
     1861 aattgattga atatattcat caatttacaa aaggagaggc cattgtcgca acggatgtag
     1921 gccagcatca aatgtggtca gcgcaatttt atccgttcca aaaagcagat aaatgggtca
     1981 cgtcaggcgg acttggaacg atgggattcg gtcttccggc ggcgatcggc gcacagctgg
     2041 ccgaaaaaga tgctactgtt gtcgcggttg tcggagacgg cggattccaa atgacgcttc
     2101 aagaactcga tgttattcgc gaattaaatc ttccggtcaa ggtagtgatt ttaaataacg
     2161 cttgtctcgg aatggtcaga cagtggcagg aaattttcta tgaagaacgt tattcagaat
     2221 ctaaattcgc ttctcagcct gacttcgtca aattgtccga agcatacggc attaaaggca
     2281 tcagaatttc atcagaagcg gaagcaaagg aaaagctgga agaggcatta acatcaagag
     2341 aacctgttgt cattgacgtg cgggttgcca gcgaagaaaa agtattcccg atggtggctc
     2401 cggggaaagg gctgcatgaa atggtggggg tgaaaccttg aaaagaatta tcacattgac
     2461 tgtggtgaac cgctccgggg tgttaaaccg gatcaccggt ctattcacaa aaaggcatta
     2521 caacattgaa agcattacag ttggacacac agaaacagcc ggcgtttcca gaatcacctt
     2581 cgtcgttcat gttgaaggtg aaaatgatgt tgaacagtta acgaaacagc tcaacaaaca
     2641 gattgatgtg ctgaaagtca cagacatcac aaatcaatcg attgtccaga gggagctggc
     2701 cttaatcaag gttgtctccg caccttcaac aagaacagag attaatggaa tcatagaacc
     2761 gtttagagcc tctgtcgttg atgtcagcag agacagcatc gttgttcagg tgacaggtga
     2821 atctaacaaa attgaagcgc ttattgagtt attaaaacct tatggcatta aagaaatcgc
     2881 gagaacaggt acaacggctt ttgcgagggg aaccagcaaa aggcgtcatc caataaaaca
     2941 atatctattg tataaaacat aacaagggag agattgaaat ggtaaaagta tattataacg
     3001 gtgatatcaa agagaacgta ttggctggaa aaacagtagc ggttatcggg tacggttcgc
     3061 aaggccacgc acatgccctg aaccttaaag aaagcggagt agacgtgatc gtcggtgtta
     3121 gacaaggaaa atctttcact caagcccaag aagacggaca taaagtattt tcagtaaaag
     3181 aagcggcagc ccaagccgaa atcatcatgg ttctgcttcc ggatgagcag cagcaaaaag
     3241 tatacgaagc tgaaatcaaa gatgaattga cagcaggaaa atcattagta ttcgctcatg
     3301 gatttaacgt gcatttccat caaattgttc ctccggcgga tgtagatgta ttcttagtgg
     3361 cccctaaagg cccgggacac ttggtaagaa gaacatatga gcaaggagct ggcgtacctg
     3421 cattgttcgc aatctatcaa gatgtgactg gagaagcaag agacaaagcc ctcgcttatg
     3481 ctaaaggaat cggcggcgca agagcgggcg tattagaaac gacatttaaa gaagaaacag
     3541 aaacagattt gttcggtgag caagcagttc tttgcggcgg attaagcgcg cttgtcaaag
     3601 ccggatttga aaccttaact gaagcaggtt atcagcctga acttgcatac ttcgagtgtc
     3661 ttcatgagct gaaattaatc gtagacctta tgtacgaaga aggacttgca ggaatgagat
     3721 attcaatctc tgacacagca cagtggggag atttcgtatc aggccctcgc gttgtggacg
     3781 ctaaagtaaa agaatctatg aaagaagtat taaaagatat ccaaaacggt acattcgcaa
     3841 aagagtggat cgtcgaaaac caagtaaacc gtcctcgttt caacgctatc aatgcaagcg
     3901 agaacgaaca tcaaatcgaa gtagtgggaa gaaagcttcg tgaaatgatg ccgtttgtga
     3961 aacaaggcaa gaagaaggaa gcggtggtct ccgttgcgca aaattaattt tttcgatacg
     4021 acgcttcgtg atggtgaaca gtcccctgga gtgaacttga atacacagga gaaacttgcc
     4081 atagctaagc agctcgaaag actcggggca gacatcattg aagcgggatt tcccgttcgt
     4141 cccgaggtga ctttttagct gttcaggaaa tcgcaagaac cattaaaaat tgttcagtaa
     4201 ctggtctggc ccgttgtgta aaaggtgata ttgatgctgc ttgggaagcg ttaaaggatg
     4261 gcgctcaacc aagaagaatt c
//