From:  lab1@vnigen.msk.su (Laboratory No1)
Subject: seeking for a postdoc position in molecular biology/biochemistry
Date: Tue, 29 Dec 1992 14:15:11 GMT
Reply-To: lab1@vnigen.msk.su
Organization: Union Institute for Genetics

                      RESUME
  PERSONAL INFORMATION:NAME ANDREY G. GALKIN /SEX MALE/DATE OF
    BIRTH JULY 2, 1965/PLACE OF BIRTH  MUROM, VLADIMIR REGION, RUSSIA,/CITIZENSHIP
    RUSSIA/ LANGUAGE RUSSIAN (NATIVE), ENGLISH(GOOD)/ADDRESS LAB1, INSTITUTE
    OF GENETICS & SELECTION OF INDUSTRIAL MICROORGANISMS,1 DOROZHNY,
    113545 MOSCOW RUSSIA; E-MAIL ADDRESS: LAB1@VNIGEN.MSK.SU
  Ph.D. DEGREE ( CHEMISTRY, MOSCOW STATE UNIVERSITY, 1991),
    Ph.D.DISSERTATION:  STADY  OF  BACTERIAL FORMATE DEHYDROGENASE BY
    GEGE ENGINEERING MEHTODS (CLONING, SEQUENCING CHARACTERIZATHION
    AND EXPRESSION OF GENE BACTERIAL FORMATE DEHIDROGENASE).
  EDUCATION: 1985 B.S.(CHEMISTRY) MOSCOW STATE UNIVERSITY, RUSSIA
             1987 M.S.(CHEMICAL ENZYMOLOGY) MOSCOW STATE  UNIVERSITY,
             RUSSIA
             1991 Ph.D.(CHEMISTRY) MOSCOW STATE UNIVERSITY, RUSSIA
  CURRENT  RESEARCH:  GENE  ENGINEERING  OF   NAD-DEPENDENT   FORMATE
         DEHYDROGENASE (SITE-DIRECTED MUTAGENESIS)
  PROFESSIONAL  EXPERIENCE:  RESEACH  SCIENTIST, DIVISION OF CHEMICAL
         ENZYMOLOGY, DEPATMENT OF CHEMISTRY, MOSCOW STATE UNIVERSITY,
         RUSSIA
  LIST OF MAIN PUBLICATIONS:
         1. LIN F,etc(1988).UTILIZATION OF CELLULOSE BY MICROMONOSPORA
    SPP.I.A NEWLY ISOLATED MICROMONOSPORA SPP. 86W-6. SYMPOSIA OF GIAM
    (EIGHTH INTERNATIONAL CONFERENCE ON GLOBAL IMPACTS OF APPLIED MICRO
    BIOLOGY) and INCABB(INTERNATIONAL CONFERENCE OF APPLIED BIOLOGY AND
    BIOTECHNOLOGY)(AUG.1-5,1988 HONG KONG). LIN F,etc. UTILIZATION OF
    CELLULOSE BY MICROMONOSPORA SPP.II.A NEWLY ISOLATED MICROMONOSPORA
    SPP.86W-16(IN PRESS). LIN FB,etc(1992). MOLECULAR CLONING AND NUCLEO
    TIDE SEQUENCE OF ENDOGLUCANASE GENE mcenA FROM Micromonospora cellu
    lolyticum 86W-16 (IN PRESS). LIN FB,etc.MOLECULAR CLONING AND SEQUENC
    ING OF ENDOTOXIN GENE FROM Bacillus thuringiensis subsp.Darmstadiensis
    61(UNFISHED RESEARCH)
  EXPERIMENTAL TECHNIQUES:MICROBIOLOGY-ISOLATION AND CHARACTERIZATION OF
    BACTERIA-MICROPHOTOGRAPHY-MUTAGENESIS/BIOCHEMISTRY -PROTEIN PURIFICA
    TION(HPLC,PAGE AND COLUMN CHROMOTOGRAPHY)-QUANTITATIVE ANALYSIS OF
    ENZYME KINETICS AND MECHANISM-AMINO ACID ANALYSIS(AUTOMATICAL AA
    ANALYZER)-SDS-PAGE/MOLECULAR GENETICS-DNA/RNA PURIFICATION(NaCl,
    SUCROSE,CsCl GRADIENTS)-POLYMERASE CHAIN REACTION-RNA TRANSCRIPTION
    IN VITRO-HYBRIDIZATION(SOUTHERN AND NORTHERN)-CONSTRUCTION OF GENE
    LIBRARY FROM GENOM OR PLASMIDS(HUGE PLASMIDS)-GENE SCREENING AND
    EXPRESSION REGULATION-PLASMID CONSTRUCTION-MAXICELL AND MINICELL-DNA
    SEQUENCE ANALYSIS(PLASMID AND PHAGE VECTOR SYSTEMS AND Erase-a-base
    SYSTEM)-FORMATION AND REGENERATION OF PROTOPLASTS-COMPUTER SKILLS/
    IMMUNOCHEMISTRY-IMMUNOPRECIPTATION ELECTROPHORESIS
  REFERENCES:PROFESSOR CHENG YUAN-RONG,DIRECTOR OF FUJIAN INSTITUTE OF
    MICROBIOLOGY  PROFESSOR YU BAO-SHENG(PH.D,JOHN'S HOPKINS UNIVERSITY),
    DIRECTOR OF BIOCHEMICAL RESEARCH LAB OF FUJIAN NORMAL UNIVERSITY
    PROFESSOR ZHENG DING,DEAN OF BIOLOGY DEPARTMENT, XIAMEN UNIVERSITY
    PROFESSOR AZIZBEKYAN R.R.,CHIEF OF LAB OF GENETICS AND SELECTION OF
    ENTOMOPATHOGENIC BACTERIA,INSTITUTE OF GENETICS AND SELECTION OF
    INDUSTRIAL MICROORGANISMS,MOSCOW RUSSIA

      LIST OF MAIN PUBLICATIONS
  1.Tishkov, V.I.,  Galkin,  A.G.  and Egorov,  A.M. NAD-dependent
formate dehydrogenase of  methylotrophic  yeast:  preparation  and
characterization.  Biochemistry (RUSSIA) 1989, v.54, N2, p.231-237
(Engl.transl.).
  2.Tishkov, V.I.,  Galkin,  A.G. and Egorov, A.M. Kinetic isotope
effect and presteady-state kinetics of the reaction  catalyzed  by
bacterial  formate  dehydrogenase.  Biochimie  1989,  v.71,  N  4,
p.551-557.
  3.Bogdanova, A.V.,  Tishkov,  V.I.,  Cherednikova, T.V., Galkin,
A.G.  and Egorov,  A.M. Immunochemical detection of microquantites
of   bacterial  formate  dehydrogenase.  Analytical  Letters,1989,
v.22(13-14), p.2761-2770.
  4.Tishkov, V.I.,  Galkin,  A.G.  and  Egorov A.M.  NAD-dependent
formate  dehydrogenase  of  methylotrophic  bacterium  Pseudomonas
sp.101:   cloning,  expression  and  structure  of  gene.  Doklady
Biochemistry (Proceedings of the Academy of Science of the  USSR),
19 1, v.317, N 3, p.345-348.
  5.Tishkov, V.I.,  Galkin,  A.G.,  Gladyshev V.N.,  Karzanov, and
Egorov A.M. Analysis of gene structure, optimization of expression
in  E.coli  and properties of recombinant formate dehydrogenase of
bacterium Pseudomonas sp.101.  Biotechnology (Russia),  19 2,  N5,
p.52-59.
  6. Tishkov, V.I., Galkin, A.G., Egorova O.A., Marchenko G.N. and
Egorov   A.M.   Site-directed  mutagenesis  of  bacterial  formate
dehydrogenase :  role of Cys255 in enzyme catalysis and  stabilty.
Doklady  Biochemistry  (Proceedings  of  the  Russian Academy of S
ience), 1993, v.328, N 3, p.137-140.

Patents
1.Tishkov, V.I.,   Berezin,   I.V.,  Egorov,  A.M.,  Galkin  A.G.,
Vaitkyavichus,  R.K.,  Kadushevichus,  V.,  Glemgha,   A.A.,   and
Petkyavichene R.  The method of formate dehydrogenase preparation.
USSR Patent Application, N 15517411 (1989).

Abstracts
 1.Tishkov, V.I.,  Galkin,  A.G.  and Egorov A.M. The mechanism of
formate  dehydrogenase  reaction:  stereoselectivity  of   hydride
transfer   and   primary  kinetic  isotope  effect.  Abstracts  if
International Conference "Chemical Physics of  Enzyme  Catalysis",
Ta linn (USSR), 1987, p.133.
 2.Tishkov, V.I.  and Galkin A.G. Study of the pre-steady kinetics
of  bacterial  formate dehydrogenase by "stopped-flow" technique.
Abstracts of 14th International Congress of Biochemistry,  Prague
(Chechoslovakia),  1988,  TH:223,  p.119.
 3.Egorov, A.M., Galkin,
A.G. and Tishkov V.I. Formate dehydrogenase gene cloning. Program
and  abstracts  of conf.  "Progress in recombinant DNA technology
and applications", June 3-8, 1990, Potosi, Missouri, USA, p.55.