From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Spinach plastid genes atpI-H-F for ATP synthase CF(O) subunits IV,
Date: 28 Dec 92 21:46:00 GMT
Distribution: bionet
Checksum: 42497 10

LOCUS       SPICPATP     4066 bp ds-DNA             ORG       02-DEC-1992
DEFINITION  Spinach plastid genes atpI-H-F for ATP synthase CF(O) subunits IV,
            III and I.
ACCESSION   X03775 M35501
KEYWORDS    ATP synthase CF0 subunit; ATP synthase proteolipid; ATP synthetase;
            atpA gene; atpF gene; atpH gene; atpI gene; synthetase.
SOURCE      Spinach chloroplast DNA.
  ORGANISM  Chloroplast Spinacia oleracea
            Eukaryota; Plantae; Embryobionta; Magnoliophyta; Magnoliopsida;
            Caryophyllidae; Caryophyllales; Chenopodiaceae.
REFERENCE   1  (bases 1793 to 2048)
  AUTHORS   Alt,J., Winter,W., Sebald,W., Moser,J.G., Schedel,R., Westhoff,P.
            and Herrmann,R.G.
  TITLE     Localization and nucleotide sequence of the gene for the ATP
            synthase proteolipid subunit on the spinach plastid chromosome
  JOURNAL   Curr. Genet. 7, 129-138 (1983)
  STANDARD  full automatic
REFERENCE   2  (bases 1 to 4066)
  AUTHORS   Hennig,J. and Herrmann,R.G.
  TITLE     Chloroplast ATP synthase of spinach contains nine nonidentical
            subunit species, six of which are encoded by plastid chromosomes in
            two operons in a phylogenetically conserved arrangement
  JOURNAL   Mol. Gen. Genet. 203, 117-128 (1986)
  STANDARD  full automatic
COMMENT     EMBL features not translated to GenBank features:
              key        from     to       description

              OPR        1369   1414       pot. stem-loop structure
              OPR        2049   2079       pot. stem-loop structure
              OPR        3775   3799       stem-loop structure pot.
                                           transcription terminator
              INVREP     2319   2326       inverted repeat A
              INVREP     2334   2341       inverted repeat A'
              INVREP     2522   2534       inverted repeat B
              INVREP     3348   3359       inverted repeat B'
              SITE       2602   2604       pot. translation stop codon
              SITE       3312   3314       pot. translation stop codon
               Between positions 2584 and 3340 six stem-loop structures
            I(1)-I(6) are described. They designate secondary structures
            resembling those of class II introns.
FEATURES             Location/Qualifiers
     exon            <2434..2578
                     /note="CFo subunit I (atpF)"
     -35_signal      206..211
                     /note="atpI -35 region"
     -10_signal      241..246
                     /note="atpI -10 region"
     RBS             348..351
                     /note="atpI ribosome binding site"
     -35_signal      1693..1698
                     /note="atpH -35 region"
     -35_signal      1710..1715
                     /note="atpH -35 region"
     -10_signal      1724..1728
                     /note="atpH -10 region"
     -10_signal      1744..1749
                     /note="atpH -10 region"
     RBS             1780..1783
                     /note="atpH ribosome binding site"
     RBS             2423..2426
                     /note="atpF ribosome binding site"
     prim_transcript 2434..3749
                     /note="primary transcript of atpF gene"
     intron          2579..3342
                     /note="put. intron (no splice consensus); putative"
     exon            3343..>3752
                     /note="CFo subunit I (atpF)"
     RBS             3810..3813
                     /note="atpA ribosome binding site"
     CDS             join(2434..2578,3343..3752)
                     /note="CFo subunit I;  (atpF)"
                     /codon_start=1
                     /translation="MKNVTDSFVFLGHWPSAGSFGFNTDILATNLINLSVVLGVLIFF
                     GKGVLSDLLDNRKQRILNTIRNSEELRGKAIEQLEKAWARLKKVEMDADQFRVNGYSE
                     IEREKMNLINSTYKTLEQFENYKNETIQFEQQKAINQVRQWVFQQALQGALGTLNSCL
                     NNELHLWTINANIGMFGAMNEITD"
     CDS             357..1100
                     /note="CFo subunit IV;  (atpI)"
                     /codon_start=1
                     /translation="MNVLSYSINPLKGLYAISGVEVGQHFYWQIGGFQIHDKALITSW
                     VVIAILLGSAAIAVWSPQTIPTGGQNFFEYVLEFIRDVSKTQIGEEYRPWVPFIGTMF
                     LFIFVSNWSGALLPWKIIQLPHGELAAPTNDINTTVALALLASVAYFYAGLTKKGLGY
                     FGKYIQPTPILLPINILEDFTKPLSLSFRLFGNILADELVVVVLVSLVPLVVPIPVMF
                     LGLFTSGIQALIFATLAAAYIGESLEGHH"
     CDS             1793..2038
                     /note="CFo subunit III;  (atpH) proteolipid"
                     /codon_start=1
                     /translation="MNPLIAAASVIAAGLAVGLASIGPGVGQGTAAGQAVEGIARQPE
                     AEGKIRGTLLLSLAFMEALTIYGLVVALALLFANPFV"
     CDS             3818..>4066
                     /note="CF1 subunit alpha;  (atpA)"
                     /codon_start=1
                     /translation="MATIRADEISKIIRERIEGYNREVKVVNTGTVLQVGDGIARIHG
                     LDEVMAGELVEFEEGTIGIALNLESNNVGVVLMGDGLMI"
BASE COUNT     1362 a    591 c    777 g   1336 t
ORIGIN      
        1 ctatttgttt aattgataca aattgtaacc cggatctcgc agctctttcg attccggcaa
       61 atgatgacgc tatagcttca attcgattaa ttcataccaa attagtattt gcaatttgtg
      121 aaggccgctc tagctatata agaaatcctt gattcataat aaaatcaaaa attgacttcc
      181 taaattctct atcggttact agattttgaa tctcaaaaac tagttcaaaa taacagggga
      241 tattatgtaa ttaatcagta tccgaaatag aaaattttaa ttaaagtaga caagtcgaga
      301 aagagctgat tgaatcaaaa taattttttt aagttatatt tctgtaagag gacaatatga
      361 atgttctatc atattcaatc aacccgctaa aggggttata tgctatatcg ggtgtggaag
      421 taggtcaaca tttttattgg caaataggag gtttccaaat ccatgacaag gcccttataa
      481 cttcttgggt tgtaattgct atcttattag gttcagctgc tatagctgtt cggagtccgc
      541 aaacaattcc gactggtggt caaaattttt ttgaatatgt ccttgaattc atccgagacg
      601 tgagcaaaac tcaaattggc gaagaatatc gcccgtgggt tccctttatt gggactatgt
      661 ttctatttat ttttgtttct aattggtcag gggctctttt accttggaaa atcatacagt
      721 tacctcatgg ggagttagcc gcacccacga acgatataaa tactactgtt gctttagctt
      781 tactcgcgtc agtagcctat ttctatgcag gtcttacaaa aaaaggatta ggttattttg
      841 gtaaatacat tcaaccaact ccaattcttt tacccattaa catcttagaa gatttcacaa
      901 aaccgctatc acttagtttt cgacttttcg gaaatatatt agcggatgaa ttagtagttg
      961 ttgttcttgt ttctttagta cctttagtgg ttcctatacc tgtcatgttt cttggcttat
     1021 ttacaagtgg tattcaggct cttatttttg caactttagc cgcagcttat ataggcgaat
     1081 ccctggaagg tcatcattga tttgtcttag gaagagttta tctcctagtt tagatatatg
     1141 tgtatgtgtg gctcaagata ctctataaag ataatctatt tagagcatat aaatatccaa
     1201 atacatacag tctagtggta atagaaaaaa cgatattcga gaagtgtaaa aaaaaaagac
     1261 gttggttagt cgagagggga tacccctgtt atatggaatc taatgactat aagctaattc
     1321 ttgcagattc gatgtttcga agaatgttca aaaattcgat tgaatttcaa atataatagg
     1381 cggtttacgt tatgtaagaa acctatgtat attttatatt agatattgac aagttatata
     1441 tgaaagaata tttaatttgc cctacttgaa tttggataga gacaccaacc gacgaagtcc
     1501 tttcctattc gtttatgact gcgaattgaa tggataaaca gacaaaatat aaaaaaaggt
     1561 cgaagaatga ttagaaaaag aaaaaggaaa tggaaaaact caagttgtat tgattcagaa
     1621 agactcaaca atataggaac taaaaaaatc aagtctttct aattatttaa taatattaat
     1681 tattatttca actggataaa tattagcaat ggaataatta agtcataatg cattggttga
     1741 ttgtatcatt aaccatttct tttttttttt tgtgtgtgtg aggaacttta tcatgaatcc
     1801 actgattgct gccgcatccg ttattgctgc tggattggct gtagggttgg cttctattgg
     1861 acctggagtt ggtcaaggta ctgctgcggg acaagctgta gaaggtattg cgagacagcc
     1921 cgaagcagaa ggaaaaatac gaggtacttt attacttagt ttagcgttta tggaagcttt
     1981 aacaatttat ggattggttg tagcattagc gcttttattt gcgaatcctt ttgtttaatc
     2041 cgaaaaggaa aagaaatagg agaaatacac atttctttta tagtcttgaa cttgcaggtt
     2101 gctttttcac atttataaga aaatatcgct cctacacaat tacttattcg ttgagaaaat
     2161 aatacacgga aaggacttaa tttgaggatg aagaattcgt gttacccact cggtttcttc
     2221 tttcctcccc ctttttagtc cgaaggagaa gtgttgcaac aaagagagta tttcgaaatt
     2281 cacatgaaac ctagtaccta attagtaatt ctataaatcc aataagtatt attcttattg
     2341 ggaatctcaa taaaaaaaat aaaattcatt tagaagtagc aaacaattga aataatacaa
     2401 cgattttttt gtttatctat aagaggagat catatgaaaa atgtaaccga ttctttcgtt
     2461 ttcttgggtc actggccatc cgccgggagt ttcgggttta ataccgatat tttagcaaca
     2521 aatctaataa atctcagtgt agtgcttggt gtattgatct tttttggaaa gggagtgtgt
     2581 gcgggttgtt tatttcaaaa ataggttgga ttcaaccaac tgtaccgctt tttttaatta
     2641 gggcgaaagg tgcatgattt cacgaatgac ttctgaaatt aataataaag aaatcatatg
     2701 taagaactag agcatttcgt gatttgttgg taaatatact ttgattctct atcaaccaat
     2761 aatgtgggac cataacatgg ttaaaactta aattgtttga agtccaggca cagcagggta
     2821 ttctttctac caccatgtta atacttaaat ataccgaata ccgaggctaa aattaaataa
     2881 agtaaagaaa catagttaca aatttttcga tatataacac tcatgtcgat aaaattgttg
     2941 gaatctctta tttattgtta taggaaaaat gtttcggctt tttttttatt aagtaaataa
     3001 atgccaaagg ctgagtcgat gacctacgta taaaataaga aacatttttg gatttgaata
     3061 aaaaaaaaac aactttgctg acaattactt atatattttt ttttgggggg tcagaagagt
     3121 tctccgaata ttctagtctt gattagtgat gggtttccat tttttgtcga ctatgaacag
     3181 agaagagagg ataagttcat tacattcaaa aaagatatgg aaatttgcca taaaaaattg
     3241 aagtaattga gcgtgagagc caaatgaatt gaaaaattca cgtttggttc gggaagggat
     3301 catgaatgtt ttgaaatgaa tggaaagata atctactttc attaagtgat ttattagata
     3361 atcgaaaaca gaggatcttg aatactattc gaaattcaga agaactacgc ggaaaggcca
     3421 ttgaacagct ggaaaaagcc cgggctcgct taaagaaagt cgaaatggac gcggatcagt
     3481 ttcgagtgaa tggatattct gaaatagaac gagaaaagat gaatttgatt aattcaactt
     3541 ataaaacttt agaacaattt gaaaattaca aaaacgaaac cattcagttt gaacaacaaa
     3601 aagcgattaa tcaagtccga cagcgggttt tccaacaagc cttacaagga gctctaggaa
     3661 ctctgaatag ttgtttgaac aatgagttac atttacggac catcaatgct aatattggca
     3721 tgtttggtgc gatgaacgaa ataactgatt agtccttcta tttaattgta ggtattattt
     3781 ttttgtttca aaaaaaaaaa gaatttaaga aagactcatg gcaaccattc gagcagatga
     3841 aattagcaaa attatccgtg aacgtattga aggatataat cgagaagtaa aggttgtaaa
     3901 taccggtaca gtgcttcaag tgggtgacgg cattgctcgt attcacggtc ttgatgaagt
     3961 aatggcaggt gaattagtag aatttgaaga gggaacaata ggtattgctc tgaatttgga
     4021 atcaaataat gttggtgttg tattaatggg tgacgggttg atgata
//