From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Rhodobacter sphaeroides isocytochrome c2 (cycI) gene, complete cds,
Date: 22 Dec 92 22:57:03 GMT
Distribution: bionet
Checksum: 43440 4

LOCUS       RCACYCI       783 bp ds-DNA             BCT       30-NOV-1992
DEFINITION  Rhodobacter sphaeroides isocytochrome c2 (cycI) gene, complete cds,
            and alcohol dehydrogenase (adhI) gene, 3' end.
ACCESSION   L02104
KEYWORDS    adhI gene; alcohol dehydrogenase; cycI gene; isocytochrome c2.
SOURCE      Rhodobacter sphaeroides DNA.
  ORGANISM  Rhodobacter sphaeroides
            Prokaryotae; Gracilicutes; Anoxyphotobacteria; Purple nonsulfur
            bacteria.
REFERENCE   1  (bases 1 to 783)
  AUTHORS   Rott,M.A., Witthuhn,V.C., Schilke,B.A., Sorrano,M., Ali,A. and
            Donohue,T.J.
  TITLE     Genetic evidence for the role of isocytochrome c2 in photosynthetic
            growth of Rhodobacter sphaeroides
  JOURNAL   Unpublished (1993)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     RBS             250..256
                     /gene="cycI"
     sig_peptide     261..323
                     /gene="cycI"
                     /codon_start=1
     mat_peptide     324..692
                     /product="isocytochrome c2"
                     /gene="cycI"
                     /codon_start=1
     protein_bind    363..377
                     /bound_moiety="heme"
                     /gene="cycI"
     CDS             <1..132
                     /gene="adhI"
                     /product="alcohol dehydrogenase"
                     /codon_start=1
                     /translation="VDWYMEGKIQIDPMITHILSLEEINKGFDLMHAGESIRSVVVF"
     CDS             261..695
                     /gene="cycI"
                     /product="isocytochrome c2"
                     /codon_start=1
                     /translation="MRLTTILAGALALGAAQAAFAEGDPAAGEKAFRKCQACHQIGAE
                     AQNKTGPVLTGVIGRPAASIEGFSYSKTLTEAAADGLVWDHAALETFLANPRKAMPGT
                     KMAFPGIKKPQELADILAYLDTFSDGETREAEETPAAAPAEG"
     CDS             697..>783
                     /note="ORF"
                     /codon_start=1
                     /translation="MPVCQGFRPRHGATGDRHTLGLKRDRSVN"
BASE COUNT      145 a    274 c    249 g    115 t
ORIGIN      
        1 gtcgactggt acatggaggg caagatccag atcgacccga tgatcaccca catcctgagc
       61 ctcgaagaga tcaacaaggg cttcgacctc atgcacgcgg gcgagtccat ccgctcggtc
      121 gtggtgttct gatcggccac ccctccaaga cggtgacgat ttcccgagta acggtgccag
      181 cgacccggcc ggtcccttga ccggccgcgg gcggcctgcc gcgcaggacg cccccgagcc
      241 atccgcaaag ggagaagacc atgagattga ccaccatcct cgccggggcg ctcgctctcg
      301 gtgccgcgca ggccgccttc gccgaaggcg acccggcggc cggcgagaag gccttccgga
      361 aatgtcaggc ctgccaccag atcggcgccg aggcgcagaa caagaccggg cccgtcctga
      421 ccggcgtcat cggtcgcccg gcggcctcga tcgagggctt cagctattcc aagaccctga
      481 ccgaggccgc ggccgatggc ctcgtctggg atcatgctgc gctcgagacc ttcctggcca
      541 atccgcgcaa ggcgatgccg ggcaccaaga tggccttccc cggcatcaag aaaccgcagg
      601 agctggccga catcctggcc tatctcgaca ccttctcgga cggggaaacg cgggaggccg
      661 aagagacccc cgcggcggcg ccggcggagg gctgaaatgc ctgtctgcca aggctttcgc
      721 cctcgtcatg gcgccaccgg cgatcgtcat actttgggct taaagcggga ccgcagcgtt
      781 aac
//