From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: P. paucimobilis gene for C alpha-dehydrogenase and beta-etherase,
Date: 15 Sep 92 10:05:09 GMT
Distribution: bionet
Checksum: 58836     8

LOCUS       PSELIG       3044 bp ds-DNA             BCT       15-SEP-1992
DEFINITION  P. paucimobilis gene for C alpha-dehydrogenase and beta-etherase,
            complete cds.
ACCESSION   D11473
KEYWORDS    C alpha-dehydrogenase;
            C alpha-oxidation of arylglycerol-beta-aryl ether; beta-etherase;
            cleavage of beta-aryl ether bond; glutathione S-transferase;
            lignin degradation; oxido-reductase.
SOURCE      Pseudomonas paucimobilis (strain SYK-6) (library: pVK100) DNA,
            clone pBE10.
  ORGANISM  Pseudomonas paucimobilis
            Prokaryotae; Gracilicutes; Scotobacteria; Pseudomonadaceae.
REFERENCE   1  (sites)
  AUTHORS   Masai,E., Katayama,Y., Kawai,S., Nishikawa,S., Yamasaki,M. and
            Morohoshi,N.
  TITLE     Cloning and sequencing of the gene for a Pseudomonas paucimobilis
            Enzyme that Cleaves beta-Aryl ether
  JOURNAL   J. Bacteriol. 173, 7950-7955 (1991)
  STANDARD  full staff_review
REFERENCE   2  (bases 1 to 3044)
  AUTHORS   Masai,E.
  JOURNAL   Unpublished (1992)
  STANDARD  full staff_review
COMMENT     These data kindly submitted in computer readable form by:
            Eiji Masai
            Lab. Wood Chemistry, Faculty of Agriculture
            Tokyo Noko University
            3-5-8 Saiwai-cho
            Fuchu-shi, Tokyo 183
            Japan
            Phone:  0423-34-6899
            Fax:    0423-34-5700
            [1]  sites.
FEATURES             Location/Qualifiers
     CDS             149..1066
                     /product="C alpha-dehydrogenase"
                     /gene="ligD"
                     /evidence=EXPERIMENTAL
                     /codon_start=1
                     /translation="MKDFQDQVAFITGGASGAGFGQAKVFGQAGAKIVVADVRAEAVE
                     KAVAELEGLGITAHGIVLDIMDREAYARAADEVEAVFGQAPTLLSNTAGVNSFGPIEK
                     TTYDDFDWIIGVNLNGVINGMVTFVPRMIASGRPGHIVTVSSLGGFMGSALAGPYSAA
                     KAASINLMEGYRQGLEKYGIGVSVCTPANIKSNIAEASRLRPAKYGTSGYVENEESIA
                     SLHSIHQHGLEPEKLAEAIKKGVEDNALYIIPYPEVREGLEKHFQAIIDSVAPMESDP
                     EGARQRVEALMAWGRDRTRVFAEGDKKGA"
     CDS             1146..1919
                     /product="beta-etherase"
                     /gene="ligF"
                     /evidence=EXPERIMENTAL
                     /codon_start=1
                     /translation="MTLKLYSFGPGANSLKPLATLYEKGLEFEQVFVDPSKFEQHSDW
                     FKKINPRGQVPALWHDGKVVTESTVICEYLEDVFPESGNSLRPADPFKRAEMRVWTKW
                     VDEYFCWCVSTIGWAFGIKAIAQKMSDEEFEEHINKNVPIPEQQLKWRRARNGFPQEM
                     LDEEFRKVGVSVARLEETLSKQDYLVDTGYSLADICNFAIANGLQRPGGFFGDYVNQE
                     KTPGLCAWLDRINARPAIKEMFEKSKREDLLKRQNEKVA"
     CDS             2041..2886
                     /product="beta-etherase"
                     /gene="ligE"
                     /evidence=EXPERIMENTAL
                     /codon_start=1
                     /translation="MARNNTITLYDLQLESGCTISPYVWRTKYALKHKGFDIDIVPGG
                     FTGILERTGGRSERVPVIVDDGEWVLDSWVIAEYLDEKYPDRPMLFEGPTQKNLMKFL
                     DNWLWSTAVGPWFRCYILDYHDLSLPQDRDYVRWSREQWFLGGQRLEDVQAGREDRLP
                     LVPPTLEPFRRILAETKWLGGDQPNFADYSALAVFLWTASVARTPPLTEDDPLRDWLD
                     RGFDLFDGLGRHPGMNPLFGLKLREGDPEPFVRQTGPAGAGGQALNKGPQTTKMPPRV
                     AEKAD"
BASE COUNT      590 a    919 c   1026 g    509 t
ORIGIN
        1 gttgacattg tccggaaatg agtggaaacc cgcttcaagc agatggcgcg ggcatcgttg
       61 ccggcgcccg tcatgggatc gcccatgaca ggccggtcaa ccggttggtt ggcgcgtcat
      121 cggccgtaga aatcaggaga gggtcggaat gaaggatttc caggatcagg tcgcgttcat
      181 cacgggcggc gcgtcgggcg ccggcttcgg ccaggcgaaa gtgttcggtc aggcaggcgc
      241 gaagatcgtg gtggcggacg tgcgggccga agcggtcgag aaggccgtcg ccgagctgga
      301 agggctcggg atcaccgcgc atggcatcgt gctcgatatc atggaccgcg aggcctatgc
      361 ccgggcggcg gacgaagtgg aggccgtgtt cggccaggcg ccgacgcttc tctccaacac
      421 cgctggcgtg aacagcttcg ggccgatcga gaagaccact tatgatgatt tcgactggat
      481 catcggcgtc aatctgaacg gcgtcatcaa cggcatggtg accttcgtgc cgcgcatgat
      541 cgcgagcggg cggccggggc acatcgtcac cgtctcgtcg ctcggcggct tcatggggag
      601 cgcgctcgcc gggccctatt cggcggccaa ggcggccagc atcaatctga tggaaggcta
      661 tcggcagggg ctagagaaat acggcatcgg cgtctccgtc tgcacgccgg ccaacatcaa
      721 gtcgaacatc gcggaagcct cgcgcctgcg tcccgcgaaa tacggcacca gcggctatgt
      781 ggagaacgag gaatcgattg cctcgctgca ctccattcac cagcacgggc tcgagccgga
      841 gaagctggcg gaagcgatca agaagggtgt cgaggacaat gctctctaca tcattcccta
      901 tcccgaagtg cgcgaaggac tggagaagca ttttcaggcc atcatcgatt cggtcgcgcc
      961 gatggagagc gatccggaag gcgcccgcca gcgggtcgag gcactgatgg cctggggacg
     1021 ggaccgcacg cgggtcttcg ccgagggcga caagaaaggc gcctgacgaa atgcacgggc
     1081 gtggcggctt aaccgccgcg cccttttcgc tcatgacgag atgacaagag atctgggaga
     1141 caggaatgac gttgaaactc tacagcttcg gtcccggggc gaactcgctc aagccgctcg
     1201 cgacgctcta cgagaagggc ctcgaattcg agcaggtctt cgtcgatccg agcaagttcg
     1261 agcagcattc ggactggttc aagaagatca atccgcgcgg tcaggtgccg gcgctctggc
     1321 atgacggcaa ggtcgtcacc gaatcgacgg tgatctgcga atatctggag gacgtgttcc
     1381 ccgagtccgg caattcgctg cgcccggccg accccttcaa gcgcgccgaa atgcgggtgt
     1441 ggaccaagtg ggtcgatgaa tatttctgct ggtgcgtctc caccatcggc tgggccttcg
     1501 gcatcaaggc gatcgcgcag aagatgagcg acgaggaatt cgaggagcac atcaacaaga
     1561 atgtgccgat ccccgagcag cagctcaaat ggcgccgcgc gcgcaacgga ttcccgcagg
     1621 agatgctgga cgaggaattc cgcaaggtcg gcgtctcggt ggcgcggctg gaagagacgc
     1681 tctcgaagca ggactatctg gtcgacacgg gttacagcct cgcggacatc tgcaatttcg
     1741 ccatcgccaa tggcctgcag cgccccggcg gcttcttcgg cgactatgtg aaccaggaaa
     1801 agacgcccgg cctgtgcgcc tggctcgacc ggatcaatgc gcgtccggcg atcaaggaaa
     1861 tgttcgagaa atcgaagcgc gaggacctgc tcaagcggca gaacgagaaa gtcgcctgaa
     1921 caggaaagcg cgtcccggcg cccgccggac aaggccagcc ggttgacgtt cgccttgtcc
     1981 ggcagcgccg ttcgcgcggc ccgggcgcgc cgctgcgttg aatgatggag aggattgccg
     2041 atggccagga acaacaccat cactctgtat gacctgcagc tggagtccgg ctgcacgatc
     2101 agcccctatg tctggcgcac caaatatgcg ctcaagcaca agggcttcga catcgacatc
     2161 gtgcccggcg gcttcaccgg cattcttgag cggaccggcg gccggtccga gcgcgtgccg
     2221 gtcatcgtgg acgatggcga gtgggtgctc gacagctggg tgatcgcgga atatctcgac
     2281 gagaaatatc ccgatcgccc catgctgttc gaggggccga cccagaagaa cctcatgaag
     2341 ttcctggaca actggctgtg gtccacggcg gtgggcccgt ggttccgctg ctatatcctc
     2401 gattatcatg atctctcgct gccgcaggac cgcgattatg tgcgctggag ccgcgagcag
     2461 tggttcctgg gcgggcagcg cctggaagac gtgcaggccg gccgcgagga tcggctgccg
     2521 ctcgtgccgc cgacgctcga gcccttccgc cgcattctcg cggaaaccaa gtggcttggc
     2581 ggcgaccagc cgaacttcgc ggactattcc gcgctggcgg tgtttctctg gaccgcgtcc
     2641 gtcgcccgca cgccgccgct gaccgaggat gatccgctgc gggactggct ggaccgtggc
     2701 ttcgacctgt ttgatgggct ggggcggcat cccggcatga acccgctgtt cgggctcaag
     2761 ctgcgggaag gcgaccccga gcctttcgtc cggcagaccg gccccgcggg cgctggcggg
     2821 caggcgctca acaaggggcc gcagaccacg aagatgccgc cgcgcgtcgc cgagaaagcg
     2881 gactgacgga aggggaggcg ggtgcgccgc ctctcgcttt cgggaaggct gaagaccgcg
     2941 gacacgagga cacaggagca agcatggccg agccacagga actgacgatc tatcacattc
     3001 ccggctgccc cttctccgag cgtgtggaaa tcatgctcga gctc
//