HDTenEightyP/NetNews huggingface dataset:
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: H.sapiens IG VL mRNA (clone mAB 63VL)
Date: 14 Aug 92 10:00:53 GMT
Distribution: bionet
Checksum: 55257     3

LOCUS       HUMVLMAB63    375 bp ss-RNA             PRI       14-AUG-1992
DEFINITION  H.sapiens IG VL mRNA (clone mAB 63VL)
ACCESSION   X54442
KEYWORDS    Ig light chain; IgM; V Lambda gene family I; autoantibody;
            joining exon; lambda light chain; monoclonal antibody;
            polyreactive; rheumatoid factor; variable region.
SOURCE      Homo sapiens RNA.
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 375)
  AUTHORS   Harindranath,N., Goldfarb,I.S., Ikematsu,H., Burastero,S.E.,
            Wilder,R.L., Notkins,A.L. and Casali,P.
  TITLE     Complete sequence of the genes encoding the VH and VL regions of
            low-high affinity monoclonal IgM and IgA1 rheumatoid factors
            produced by CD5+ B cells from a rheumatoid arthritis patient
  JOURNAL   Int. Immunol. 3, 865-875 (1991)
  STANDARD  full staff_entry
COMMENT     *source: stage=adult; *source: cell_type=B-cell heterohybridoma;
            *source: cell_line=Ab 63; *source: clone_library=lambda gt11;
            *source: clone=mAB 63 VL; *source: isolate=rheumatoid arthritis
            patient (ML); *source: chromosome=22q11.1-q11.2; *source:
            is_germline=N;
            See also X54435-X54438 & X54441-47
            From EMBL    entry HSVLMAB63;  dated 07-FEB-1992.
FEATURES             Location/Qualifiers
     CDS             <1..>375
                     /product="Ig light chain precursor"
                     /codon_start=1
                     /translation="TPLWLTLLTLCIGSVVSSELTQDPPVSVALGQTVRITCQGDSLR
                     SYYASWYQEKPGQAPVLVIYGKNNRPSGIPDRFSGSSSGNTASLTITGAQAEDEADYY
                     CNSRDSSGNHYVFGTGTKVTVLG"
     sig_peptide     <1..51
                     /codon_start=1
     mat_peptide     52..>375
                     /product="Ig light chain (V-J region)"
                     /codon_start=1
     misc_feature    337..338
                     /note="V-region end/J-region start"
BASE COUNT       86 a    106 c     95 g     88 t
ORIGIN
        1 acccctcttt ggctcactct cctcactctt tgcataggtt ctgtggtttc ttctgagctg
       61 actcaggacc ctcctgtgtc tgtggccttg ggacagacag tcaggatcac atgccaagga
      121 gacagcctca gaagctatta tgcaagctgg taccaggaga agccaggaca ggcccctgta
      181 cttgtcatct atggtaaaaa caaccggccc tcagggatcc cagaccgatt ctctgggtcc
      241 agctcaggaa acacagcttc cttgaccatc actggggctc aggcggaaga tgaggctgac
      301 tattactgta actcccggga cagcagtggt aaccattatg tcttcggaac tgggaccaag
      361 gtcaccgtcc taggt
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Hylobates lar MHC DQB1*02 gene, partial cds, clone Gidqb102.
Date: 12 Aug 92 10:00:37 GMT
Distribution: bionet
Checksum: 16279     2

LOCUS       GIBMHDQBB     246 bp ds-DNA             PRI       12-AUG-1992
DEFINITION  Hylobates lar MHC DQB1*02 gene, partial cds, clone Gidqb102.
ACCESSION   M81275
KEYWORDS    major histocompatibility complex.
SOURCE      Hylobates lar (individual_isolate Gibby) blood DNA.
  ORGANISM  Hylobates lar
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hylobatidae.
REFERENCE   1  (bases 1 to 246)
  AUTHORS   Bontrop,R.E., Jonker,M., van Weeren,P., Kenter,M. and Otting,N.
  TITLE     MHC-DQB repertoire variation in hominoid and old world primate
            species
  JOURNAL   J. Immunol. 149, 461-470 (1992)
  STANDARD  full staff_entry
FEATURES             Location/Qualifiers
     CDS             <1..>246
                     /gene="MHC DQ-beta 1"
                     /label=exon 2
                     /codon_start=1
                     /translation="YQFKGMCYFTNGTERVRGVARYIYNREEYVRFDSDVGVYRALTP
                     LGRPDAEYWNSQKDILGRTRAEVDTVCRHNYQSELRTT"
BASE COUNT       55 a     66 c     88 g     37 t
ORIGIN
        1 taccagttta agggcatgtg ctacttcacc aacgggacgg agcgcgtgcg aggtgtggcc
       61 agatacatct ataaccgaga agagtacgtg cgcttcgaca gcgacgtggg ggtgtaccgg
      121 gcgctgacgc cgctggggcg gcctgacgcc gagtactgga acagccagaa ggacatcctg
      181 gggaggaccc gggcggaggt ggatacggtg tgcagacaca actaccagtc ggagctccgc
      241 acgacc
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human chromosome 4 sequence-tagged site STS4-85
Date: 21 Aug 92 10:11:13 GMT
Distribution: bionet
Checksum: 19528     3

LOCUS       HUM4STS85     227 bp ds-DNA             PRI       21-AUG-1992
DEFINITION  Human chromosome 4 sequence-tagged site STS4-85
ACCESSION   L00916
KEYWORDS    STS; human chromosome 4; sequence tagged site.
SOURCE      Homo sapiens, clone L4-25 from the C25 lambda library (Pritchard,
            Genomics 4, 408, 1989); vector - Lambda Dash II (Stratagene)
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 227)
  AUTHORS   Goold,R.D., diSibio,G., Dugaiczyk,A., Smith,K.A., Xu,H.,
            Magrane,G., Lang,D.B., Cox,D.R., Masters,S.B. and Myers,R.M.
  TITLE     Sequence-Tagged Sites for Human Chromosome 4
  JOURNAL   Unpublished (1992) see COMMENT for author address
  STANDARD  full automatic
COMMENT     PCR components: 25 ng of human genomic DNA, 10 pmol of each
            oligonucleotide, 200 micro-M dNTPs, 0.25 U Taq  polymerase (Cetus)
            in 10 micro-l of 50 mM KCl-20 mM  Tris-HCl, pH 8.3 (at room temp),
            2.5 mM MgCl-2.  Initial denaturation at 94degC for 1.5 min, then 30
            cycles of  94degC for 15 sec, 62degC for 23 sec, and 72degC for 30
            sec, followed by a final extension at 72degC for 3.5 min, using a
            Perkin-Elmer 9600 thermocycler. PCR-amplified product size 210 bp.
            This sequence is from T3 end of lambda clone.  M95306 has sequence
            from the T7 end of this clone.  GDB Locus: D4S337.
            Sequence submitted by:
            Human Genome Mapping Center
            Box 0925
            University of California San Francisco
            San Francisco, CA 94143-0925  USA
            Phone: (415) 502-1612   Fax: (415) 476-8391
            e-mail: hgmcprobes@cgl.ucsf.edu
FEATURES             Location/Qualifiers
     misc_feature    1..24
                     /label=Primer1
     misc_feature    complement(204..227)
                     /label=Primer2
BASE COUNT       63 a     41 c     42 g     81 t
ORIGIN
        1 cttatggtct aaggttggag cctgagaatg acaggctttt cataaagtct tctggagaag
       61 tgtttataat gttacctatt ttacatgcta acagagattc taaggggaaa agactctaac
      121 tcatataaca gactttaaca tagattccag gtagccaatt gtttttcaag tattgctgtt
      181 tatgttcatt agaggcttgt tctcctgttc tccttatcaa tcacctc
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Rattus norvegicus pore-forming calcium channel alpha-1 subunit
Date: 19 Aug 92 10:03:40 GMT
Distribution: bionet
Checksum: 16271     3

LOCUS       RATCCASA      850 bp ss-mRNA            ROD       19-AUG-1992
DEFINITION  Rattus norvegicus pore-forming calcium channel alpha-1 subunit
            mRNA, partial cds.
ACCESSION   M99221
KEYWORDS    calcium channel alpha-1 subunit.
SOURCE      Rattus norvegicus (strain CD) male adult kidney cDNA to mRNA.
  ORGANISM  Rattus norvegicus
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Rodentia; Myomorpha; Muridae; Murinae.
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Yu,A.S., Hebert,S.C., Brenner,B.M. and Lytton,J.
  TITLE     Molecular characterization and nephron distribution of a family of
            transcripts encoding the pore-forming subunit of Ca2+ channels in
            the kidney
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. (1992) In press
  STANDARD  full staff_entry
FEATURES             Location/Qualifiers
     CDS             <1..>850
                     /note="'PCR amplified splice variant of neuroendocrine
                     L-type calcium channel'"
                     /product="calcium channel alpha-1 subunit"
                     /codon_start=2
                     /translation="FYRCTDEAKSNPEECRGLFILYKDGDVDSPVVRERIWQNSDFNF
                     DNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIVAFF
                     MMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWY
                     VVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIA
                     FKPKNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAM
                     LFFIYAV"
     misc_feature    667..668
                     /note="'splice variant with a 129 bp deletion at this
                     site'"
BASE COUNT      201 a    220 c    200 g    229 t
ORIGIN
        1 gttctaccgt tgcacagatg aggccaaaag taaccccgag gagtgcaggg ggcttttcat
       61 cctttataag gacggcgatg tcgacagtcc cgtggtccgt gagaggatct ggcaaaacag
      121 tgatttcaat ttcgacaatg tcctttcggc tatgatggcg ctcttcacgg tctcgacttt
      181 tgagggctgg cccgcgttgc tgtacaaagc tatcgactca aacggagaga acgttggtcc
      241 tgtctacaac taccgtgtgg agatctccat cttcttcatt atctacatca tcatcgtggc
      301 cttcttcatg atgaatatct tcgtgggctt cgtcatcgtc accttccagg agcagggaga
      361 aaaggagtat aagaactgtg agctggacaa aaatcagcgt cagtgtgtgg aatatgcctt
      421 gaaggcccgc cccttaagga gatacatccc caaaaaccca taccagtaca agttctggta
      481 cgtggtgaac tcctcgcctt tcgaatatat gatgtttgtc ctcatcatgc tcaacacgct
      541 ctgcctggcc atgcagcact atgagcaatc caagatgttc aatgacgcca tggacattct
      601 gaacatggtc ttcacggggg tcttcaccgt tgagatggtt ttgaaagtca tcgcatttaa
      661 gcccaagaac tctgaagaga gcaatagaat ctccatcacc tttttccgtc ttttccgagt
      721 gatgcggttg gtgaagcttc tcagcagagg ggaaggcatc cggactctgc tatggacctt
      781 cattaagtcc ttccaggcac tcccatatgt cgccctcctc attgccatgc tcttcttcat
      841 ctacgccgtc
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Parantechinus apicalis cytochrome b gene, partial cds.
Date: 20 Aug 92 10:04:29 GMT
Distribution: bionet
Checksum: 40807     3

LOCUS       PUSCYTOB      649 bp ds-DNA             ORG       20-AUG-1992
DEFINITION  Parantechinus apicalis cytochrome b gene, partial cds.
ACCESSION   M99457
KEYWORDS    cytochrome b.
SOURCE      Mitochondrion Parantechinus apicalis (chromosome Mitochondrion
            Parantechinus apicalis) DNA.
  ORGANISM  Mitochondrion Parantechinus apicalis
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Metatheria; Marsupialia; Marsupicarnivora; Dasyuridae;
            Phascogalinae.
REFERENCE   1  (bases 1 to 649)
  AUTHORS   Krajewski,C., Driskell,A.C., Baverstock,P.R. and Braun,M.J.
  TITLE     Phylogenetic relationships of the thylacine (Marsupialia:
            Thylacinidae) among dasyuroid marsupials: evidence from
            cytochrome-b DNA sequences
  JOURNAL   Proc. R. Soc. Lond., B, Biol. Sci. (1992) In press
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     CDS             1..649
                     /product="cytochrome b"
                     /note="putative"
                     /codon_start=2
                     /translation="FGSLLGICLIIQVLTGLFLAMHYTSDTLTAFSSVAHICRDVNHG
                     WLLRNLHANGASMFFMCLFLHVGRGIYYGSYLYKETWNIGVILLLTVMATAFVGYVLP
                     WGQMSFWGATVITNLLSAIPYIGTTLAEWIWGGFAVDKATLTRFFGFHFILPFIITAL
                     AMVHLLFLHETGSNNPSGINPDADKIPFHPYYTIKDALGLMFLLLALLXXALFSPD"
                     /citation=[1]
                     /partial
BASE COUNT      173 a    191 c     92 g    190 t      3 others
ORIGIN
        1 ctttggatcc ttactaggaa tttgcctaat tatccaagtc ctcacaggcc tcttcctagc
       61 aatacactac acctcagata ctctaacagc cttctcttcc gtagctcaca tttgccgcga
      121 tgttaaccac ggctgactcc tccgcaatct acatgctaat ggagcctcaa tattcttcat
      181 atgcttattc ctacatgtag gccgaggaat ttactacggt tcctatctct acaaagaaac
      241 atgaaacatt ggcgttatcc ttctacttac tgtcatagcc actgcattcg tagggtacgt
      301 ccttccttga ggccaaatat cattttgagg agctaccgta attacaaacc tactatcagc
      361 catcccctac atcggcacca ccttagcaga atggatttga ggtggtttcg cagtagacaa
      421 agcaaccctt acacgattct tcggcttcca cttcattcta ccctttatca tcacagcact
      481 tgctatagtc caccttctat tcctacatga aacaggatct aataacccct caggaattaa
      541 cccagacgca gataaaatcc cattccaccc ctactacact attaaagatg ctttaggcct
      601 aatatttctc ctattagccc ttctacnnnt agcccttttc tcaccagac
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human Ig germline H-chain gene V region, partial cds.
Date: 22 Aug 92 10:00:37 GMT
Distribution: bionet
Checksum: 24737     2

LOCUS       HUM2IGVH      434 bp ds-DNA             PRI       22-AUG-1992
DEFINITION  Human Ig germline H-chain gene V region, partial cds.
ACCESSION   M99398
KEYWORDS    immunoglobulin heavy chain subgroup VH-III;
            immunoglobulin heavy chain variable region.
SOURCE      Homo sapiens (individual_isolate LS) adult blood DNA.
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 434)
  AUTHORS   Adderson,E.E., Carroll,W.L. and Shackelford,P.G.
  TITLE     The human VH3b gene subfamily is highly polymorphic
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     CDS             join(1..13,117..434)
                     /product="immunoglobulin heavy chain variable region"
                     /gene="IGHV@"
                     /codon_start=1
                     /translation="AILKGVQCEVQLVESGGGLVKPGGSLRLSCAASGFTFSNAWMSW
                     VRQAPGKGLEWVGRIKSKTDGGTTDYAAPVKGRFTISRDDSKNTLYLQMNSLKTEDTA
                     VYYCTTDT"
                     /partial
     sig_peptide     1..13
                     /gene="IGHV@"
                     /codon_start=1
                     /partial
     intron          14..116
                     /gene="IGHV@"
BASE COUNT      113 a     84 c    134 g    103 t
ORIGIN
        1 gctattttaa aaggtgattt atggagaact agagagatta agtgtgagtg gacgtgagtg
       61 agagaaacag tggatatgtg tggcagtttc tgatcttagt gtctctgtgt ttgcaggtgt
      121 ccagtgtgag gtgcagctgg tggagtctgg gggaggcttg gtaaagcctg gggggtccct
      181 tagactctcc tgtgcagcct ctggattcac tttcagtaac gcctggatga gctgggtccg
      241 ccaggctcca gggaaggggc tggagtgggt tggccgtatt aaaagcaaaa ctgatggtgg
      301 gacaacagac tacgctgcac ccgtgaaagg cagattcacc atctcaagag atgattcaaa
      361 aaacacgctg tatctgcaaa tgaacagcct gaaaaccgag gacacagccg tgtattactg
      421 taccacagac acag
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: M.musculus parvalbumin gene exon 3 3'-part
Date: 22 Aug 92 10:01:14 GMT
Distribution: bionet
Checksum: 62969     2

LOCUS       MUSPVAEX32     27 bp ds-DNA             ROD       22-AUG-1992
DEFINITION  M.musculus parvalbumin gene exon 3 3'-part
ACCESSION   X62830
KEYWORDS    cytosolic calcium binding protein; parvalbumin; pva gene.
SOURCE      Mus musculus DNA.
  ORGANISM  Mus musculus
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Rodentia; Myomorpha; Muridae; Murinae.
REFERENCE   1  (bases 1 to 27)
  AUTHORS   Schleef,M.
  JOURNAL   Unpublished (1991)
  STANDARD  simple staff_entry
REFERENCE   2  (sites)
  AUTHORS   Schleef,M., Zuehlke,C.H., Jockusch,H. and Schoeffl,F.
  TITLE     The structure of the mouse parvalbumin gene
  JOURNAL   Mamm. Genome 3, 217-225 (1992)
  STANDARD  full staff_review
COMMENT     *source: strain=ssp. domesticus, strain=A2G; *source: stage=adult;
            *source: chromosome=15;
            From EMBL    entry MMPVAEX32;  dated 01-JUL-1992.
FEATURES             Location/Qualifiers
     prim_transcript <1..>27
                     /usedin=X62826:pva_transcr
     exon            <1..7
                     /number=3
                     /usedin=X62826:pva_mRNA
                     /usedin=X62827:pva_CDS
                     /note="3'end"
                     /gene="pva"
     intron          8..>27
                     /number=3
                     /note="5'end"
BASE COUNT        6 a      2 c     14 g      5 t
ORIGIN
        1 agctggggta aattgaggat ggggcgg
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human chromosome 4 sequence-tagged site STS4-94
Date: 21 Aug 92 10:11:21 GMT
Distribution: bionet
Checksum: 53236     3

LOCUS       HUM4STS94     430 bp ds-DNA             PRI       21-AUG-1992
DEFINITION  Human chromosome 4 sequence-tagged site STS4-94
ACCESSION   M95251
KEYWORDS    STS; human chromosome 4; sequence tagged site.
SOURCE      Homo sapiens, clone C4-154 from Los Alamos National Laboratory
            chromosome 4 cosmid library, plate 4-1-2R, pos 4A; vector SuperCos
            1 (Stratagene)
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 430)
  AUTHORS   Goold,R.D., diSibio,G., Dugaiczyk,A., Smith,K.A., Xu,H.,
            Magrane,G., Lang,D.B., Cox,D.R., Masters,S.B. and Myers,R.M.
  TITLE     Sequence-Tagged Sites for Human Chromosome 4
  JOURNAL   Unpublished (1992) see COMMENT for author address
  STANDARD  full automatic
COMMENT     PCR components: 25 ng of human genomic DNA, 10 pmol of each
            oligonucleotide, 200 micro-M dNTPs, 0.25 U Taq  polymerase (Cetus)
            in 10 micro-l of 50 mM KCl-20 mM  Tris-HCl, pH 8.3 (at room temp),
            2.5 mM MgCl-2.  Initial denaturation at 94degC for 1.5 min, then 30
            cycles of  94degC for 15 sec, 62degC for 23 sec, and 72degC for 30
            sec, followed by a final extension at 72degC for 3.5 min, using a
            Perkin-Elmer 9600 thermocycler. PCR-amplified product size 106 bp.
            Sequence submitted by:
            Human Genome Mapping Center
            Box 0925
            University of California San Francisco
            San Francisco, CA 94143-0925  USA
            Phone: (415) 502-1612   Fax: (415) 476-8391
            e-mail: hgmcprobes@cgl.ucsf.edu
FEATURES             Location/Qualifiers
     misc_feature    43..69
                     /label=Primer1
     misc_feature    complement(125..148)
                     /label=Primer2
BASE COUNT      137 a     79 c     59 g    104 t     51 others
ORIGIN
        1 acancggaaa angtcaataa atngactaca taagggntta anctccatta tgtcaaaaat
       61 atacacaaaa tattaanggc aaatgaggta tatctgcaac aaatatatag taaaatgnta
      121 atgtccttaa tgtggaaaga ggactccaan gatnaaaata atacagnttc cccagnaaaa
      181 acaantggcc aagggacatg aacagacaat ncatggaaaa ntntntttag gatatncaac
      241 tccactggta nttaactaac tannnataaa ntaaaacttg ggnctcccaa ntnggcantc
      301 attaccttnn tntnttangt ngtgggnnng angtnttang tntatgctca nttctnacag
      361 ncccncctga aacgtatctc tgtgtttncc acatcntnca tgttnaccca ncctccccnc
      421 tctntccntc
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human chromosome 4 sequence-tagged site STS4-185
Date: 21 Aug 92 10:07:30 GMT
Distribution: bionet
Checksum: 49251     3

LOCUS       HUM4STS185    355 bp ds-DNA             PRI       21-AUG-1992
DEFINITION  Human chromosome 4 sequence-tagged site STS4-185
ACCESSION   L00744
KEYWORDS    STS; human chromosome 4; sequence tagged site.
SOURCE      Homo sapiens, clone p4-125 from a chromosome 4 plasmid library;
            vector pBluescript II KS + (Stratagene)
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 355)
  AUTHORS   Goold,R.D., diSibio,G., Dugaiczyk,A., Smith,K.A., Xu,H.,
            Magrane,G., Lang,D.B., Cox,D.R., Masters,S.B. and Myers,R.M.
  TITLE     Sequence-Tagged Sites for Human Chromosome 4
  JOURNAL   Unpublished (1992) see COMMENT for author address
  STANDARD  full automatic
COMMENT     PCR components: 25 ng of human genomic DNA, 10 pmol of each
            oligonucleotide, 200 micro-M dNTPs, 0.25 U Taq  polymerase (Cetus)
            in 10 micro-l of 50 mM KCl-20 mM  Tris-HCl, pH 8.3 (at room temp),
            2.5 mM MgCl-2.  Initial denaturation at 94degC for 1.5 min, then 30
            cycles of  94degC for 15 sec, 62degC for 23 sec, and 72degC for 30
            sec, followed by a final extension at 72degC for 3.5 min, using a
            Perkin-Elmer 9600 thermocycler. PCR-amplified product size 129 bp.
            Sequence submitted by:
            Human Genome Mapping Center
            Box 0925
            University of California San Francisco
            San Francisco, CA 94143-0925  USA
            Phone: (415) 502-1612   Fax: (415) 476-8391
            e-mail: hgmcprobes@cgl.ucsf.edu
FEATURES             Location/Qualifiers
     misc_feature    102..123
                     /label=Primer1
     misc_feature    complement(210..230)
                     /label=Primer2
BASE COUNT       65 a     96 c    104 g     74 t     16 others
ORIGIN
        1 tgggggggcc aggagtgggt accgggcccc ccctcgaggt cgacggtatc gataagcttg
       61 atatcgaatt cctgcagccc cctcaacccg aagggagcct cccagctctg tcctctggtt
      121 cctgcccagt ccactaggat gatgtatttt aaccatgacc acctttccca gttgccctac
      181 ctggagagct gcaagccttg gggggagagg aactgcccac cttggtcctg gggcagggag
      241 agntcactga agggaaggac acaagtcttg ttggattggn gggggggatc cactagntct
      301 agagangccn acaccgnngt gngngntcta ntncgcccct nttgtnantc gctnt
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Lentinus ponderosus ribosomal RNA (14LR3) large subunit, partial.
Date: 19 Aug 92 10:02:22 GMT
Distribution: bionet
Checksum: 16278     2

LOCUS       LENRRLST      128 bp ss-rRNA            RNA       19-AUG-1992
DEFINITION  Lentinus ponderosus ribosomal RNA (14LR3) large subunit, partial.
ACCESSION   M98584
KEYWORDS    large subunit ribosomal RNA.
SOURCE      Lentinus ponderosus (library: Duke culture collection 585) cDNA to
            rRNA.
  ORGANISM  Lentinus ponderosus
            Eukaryota; Fungi; Eumycota; Basidiomycotina; Hymenomycetes;
            Agaricales; Tricholomataceae.
REFERENCE   1  (bases 1 to 128)
  AUTHORS   Hibbett,D.S. and Vilgalys,R.
  TITLE     Phylogenetic relationships of the basidiomycete genus Lentinus
            inferred from molecular and morphological characters
  JOURNAL   Unpublished (1992)
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     rRNA            <1..>128
                     /note="Aligns to region between positions 371 to 597
                     inSaccharomyces cerevisiae large subunit nuclear rRNA."
                     /product="large subunit ribosomal RNA"
                     /citation=[1]
BASE COUNT       19 a     26 c     44 g     39 t
ORIGIN
        1 ttcagcgctt tccgcttggt gtattttccg gttgacgggc cagcatcgat tttgaccgct
       61 ggataagggc taggggaatg tggcaccttc gggtgtgtta tagcctgtgg tcgtatacag
      121 tggtcggg
//