From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: H.roretzi muscle actin gene.
Date: 3 Jan 93 22:15:05 GMT
Distribution: bionet
Checksum: 20082 5

LOCUS       HROMA2       1787 bp ds-DNA             INV       23-DEC-1992
DEFINITION  H.roretzi muscle actin gene.
ACCESSION   D10886
KEYWORDS    muscle actin.
SOURCE      H.roretzi DNA, clone lambda ACT11.
  ORGANISM  Halocynthia roretzi
            Eukaryota; Animalia; Chorqata; Tunicata; Ascidiacea;
            Stolidobranchia; Pyuridae.
REFERENCE   1  (bases 1 to 1787)
  AUTHORS   Kusakabe,T.
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
REFERENCE   2  (sites)
  AUTHORS   Kusakabe,T., Makabe,K.W. and Satoh,N.
  TITLE     Tunicate muscle actin genes: structure and organizarion as a gene
            cluster
  JOURNAL   J. Mol. Biol. 227, 955-960 (1992)
  STANDARD  full automatic
COMMENT     Submitted (19-May-1992) to DDBJ by: Takehiro Kusakabe Department of
            Zoology Faculty of Science Kyoto University Oiwake-cho,
            Kitashirakawa Sakyo-ku, Kyoto 606-01 Japan Phone:  075-751-6149
            Fax:    075-753-4081

               [2]  sites.
FEATURES             Location/Qualifiers
     misc_feature    89..98
                     /note="CArG box"
     TATA_signal     176..182
     prim_transcript 206..1710
     exon            206..738
                     /number=1
     intron          739..819
                     /number=1
     exon            820..1173
                     /number=2
     intron          1174..1284
                     /number=2
     exon            1285..1710
                     /number=3
     polyA_signal    1693..1698
     CDS             join(282..738,820..1173,1285..1610)
                     /gene="HrMA2"
                     /product="muscle actin"
                     /codon_start=1
                     /translation="MSDGEEDTTAIVCDNGSGLVKSGFAGDDAPRAVFPSIVGRPRHQ
                     GVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPE
                     EHPTLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQAVLSLYASGRTTGIVLDAGD
                     GVSHNVPIYEGYALPHAIARLDLAGRDLTDYLMKILTERGYSFVTTAEREIVRDIKEK
                     LCYVALDFEQEMATAASSTSLEKSYELPDGQVITIGNERFRCPETLFQPSFIGMESAG
                     IHETTYNSIMKCDIDIRKDLYANNVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIA
                     PPERKYSVWIGGSILASLSTFQQMWISKQEYDEAGPSIVHRKCF"
BASE COUNT      539 a    392 c    331 g    525 t
ORIGIN      
        1 acaccaaaga cacacaagtg tagaactcaa ttgttcgcac gtaccaccag ttgaacgata
       61 tatcccacca tgctattctt aagttgctcc ttttatggtc atcgcacttc tcgttacgaa
      121 ttgcgcgtga taacaactgt cttcattttt tttttcgtta ctccgttact ttcagtatat
      181 aagccctcta tcgttttcat atattcatca ccaagcgtgt acagtcaatc aatcaactac
      241 atcctccaag ttctatctgt actaaataac aaaacaaaat catgtctgat ggtgaagaag
      301 atacaaccgc aatcgtctgc gacaatggat caggattggt caagtctggt tttgccggtg
      361 atgacgcacc aagagcagtt tttccatcaa tcgttggtcg tccaagacat caaggtgtca
      421 tggtcggtat gggacagaag gacagctacg taggagatga ggctcaaagc aaaagaggaa
      481 tcctcacttt gaaatatccc attgaacatg gtatcatcac aaactgggat gatatggaaa
      541 agatctggca tcacaccttt tacaatgaac tccgtgtcgc tcctgaagag catccaactc
      601 ttctcacaga agctccgttg aatcccaagg caaatagaga aaagatgaca caaattatgt
      661 tcgagacctt caacgtccca gccatgtatg tcgccattca agctgtgcta tcactatatg
      721 cttctggaag aacaactggt gagtatttga caatttgtaa tgtaattagt tcgttcgtct
      781 gtggcaacaa tgctcaaact gatttctgta cgattatagg tattgtattg gatgctggtg
      841 atggtgtctc ccacaatgtc cccatctacg aaggatacgc tcttcctcat gccatcgcaa
      901 ggttagattt ggccggtcgt gatttaactg attacttgat gaagattctc acagaaagag
      961 gatattcctt cgtcacaact gccgaacgtg aaattgttcg tgatatcaag gaaaagttat
     1021 gctacgtcgc acttgacttc gaacaagaga tggcaactgc ggcttcgtcg acttcactcg
     1081 aaaagagcta cgaacttccc gatggacaag ttatcactat tggaaacgaa agattccgtt
     1141 gcccggaaac tctttttcaa ccgtcattca ttggtaagtt gaaagcttca tcatctgttt
     1201 atcaacgaag atatacaaag gaaaggaaat acttcgcaat ttcgagttca taatcgacat
     1261 aataataaat ttgttatttg tcaggtatgg aatcagctgg tattcacgaa accacgtaca
     1321 attccatcat gaaatgcgat attgacatcc gtaaggatct ttacgctaac aacgttctct
     1381 ctggtggaac caccatgtat cctggtattg ctgatcgtat gcaaaaagaa atcacagccc
     1441 ttgctccaag taccatgaag atcaaaatca ttgctcctcc tgaacgtaaa tattctgtct
     1501 ggattggagg atctatcctt gcttccctct ctaccttcca acagatgtgg attagcaaac
     1561 aagaatacga tgaagccgga ccatccattg ttcatagaaa gtgtttctaa ttcacatctc
     1621 cagctatgca ccatccactg tacggactta aattattacc atttcaatgg attactcttt
     1681 gttatgattt caaataaaat tcgcctctgt aaacaattga taacaataat ttcttaagtt
     1741 attagggaga aattttattc catagaccta acatgattat actattc
//