From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: S.cerevisiae heat shock protein 70 (SSA3) gene (clone pSSA3H),
Date: 21 Jul 92 10:09:08 GMT
Distribution: bionet
Checksum: 36082     6

LOCUS       YSCHSP70A    2141 bp ds-DNA             PLN       21-JUL-1992
DEFINITION  S.cerevisiae heat shock protein 70 (SSA3) gene (clone pSSA3H),
            complete cds.
ACCESSION   M97225
KEYWORDS    ATP binding; cytoplasmic protein; heat shock protein 70.
SOURCE      Saccharomyces cerevisiae (strain S288C) DNA.
  ORGANISM  Saccharomyces cerevisiae
            Eukaryota; Fungi; Eumycota; Hemiascomycetes; Endomycetales;
            Saccharomycetaceae.
REFERENCE   1  (sites)
  AUTHORS   Englisch,U., Englisch,E., Markmeyer,P., Schischkoff,J.,
            Sternbach,H., Kratzin,H. and Cramer,F.
  TITLE     Structure of the yeast isoleucyl-tRNA synthetase gene (ILS1)
  JOURNAL   Biol. Chem. Hoppe-Seyler 368, 971-979 (1987)
  STANDARD  full staff_review
REFERENCE   2  (sites)
  AUTHORS   Martindale,D.W., Gu,Z.M. and Csank,C.
  TITLE     Isolation and complete sequence of the yeast isoleucyl-tRNA
            synthetase gene (ILS1)
  JOURNAL   Curr. Genet. 15, 99-106 (1989)
  STANDARD  full staff_review
REFERENCE   3  (sites)
  AUTHORS   Boorstein,W.R. and Craig,E.A.
  TITLE     Transcriptional regulation of SSA3, an HSP70 gene from
            Saccharomyces cerevisiae
  JOURNAL   Mol. Cell. Biol. 10, 3262-3267 (1990)
  STANDARD  full staff_review
REFERENCE   4  (bases 1 to 2141)
  AUTHORS   Boorstein,W.R.
  JOURNAL   Unpublished (1992)
  STANDARD  full staff_entry
FEATURES             Location/Qualifiers
     CDS             1..1950
                     /product="heat shock protein 70"
                     /gene="SSA3"
                     /codon_start=1
                     /translation="MSRAVGIDLGTTYSCVAHFSNDRVEIIANDQGNRTTPSYVAFTD
                     TERLIGDAAKNQAAINPHNTVFDAKRLIGRKFDDPEVTTDAKHFPFKVISRDGKPVVQ
                     VEYKGETKTFTPEEISSMVLSKMKETAENYLGTTVNDAVVTVPAYFNDSQRQATKDAG
                     TIAGMNVLRIINEPTAAAIAYGLDKKGRAEHNVLIFDLGGGTFDVSLLSIDEGVFEVK
                     ATAGDTHLGGEDFDNRLVNHLATEFKRKTKKDISNNQRSLRRLRTAAERAKRALSSSS
                     QTSIEIDSLFEGMDFYTSLTRARFEELCADLFRSTLEPVEKVLKDSKLDKSQIDEIVL
                     VGGSTRIPKIQKLVSDFFNGKEPNRSINPDEAVAYGAAVQAAILTGDQSTKTQDLLLL
                     DVAPLSLGIETAGGIMTKLIPRNSTIPTKKSETFSTYADNQPGVLIQVFEGERTRTKD
                     NNLLGKFELSGIPPAPRGVPQIDVTFDIDANGILNVSALEKGTGKSNKITITNDKGRL
                     SKDDIDRMVSEAEKYRADDEREAERVQAKNQLESYAFTLKNTINEASFKEKVGEDDAK
                     RLETASQETIDWLDASQAASTDEYKDRQKELEGIANPIMTKFYGAGAGAGPGAGESGG
                     FPGSMPNSGATGGGEDTGPTVEEVD"
BASE COUNT      670 a    377 c    494 g    600 t
ORIGIN
        1 atgtctagag cagttggtat tgatttggga acaacttact cgtgtgttgc tcatttttcc
       61 aatgataggg tagagataat tgcaaatgat caaggtaata ggaccactcc atcgtatgtg
      121 gctttcacag acaccgaaag attaattggt gacgccgcca aaaatcaagc tgcaatcaat
      181 cctcataata cagtttttga tgcaaagcgg ttaattggtc gtaaatttga tgatcctgaa
      241 gtgacgacag atgccaagca cttccctttc aaagttatat ccagagatgg taaacctgta
      301 gtgcaagtag aatataaggg tgaaacgaaa acatttacgc ctgaggaaat ttcttccatg
      361 gttttaagca aaatgaagga aactgctgag aactatttgg gaactacggt caatgatgct
      421 gttgtaactg ttcctgcata tttcaatgat tctcaaagac aagccactaa ggatgcagga
      481 actattgcag ggatgaacgt tttacgtatt atcaatgaac ccactgcagc agcaattgct
      541 tatggcttgg ataagaaagg cagggctgag cacaatgtcc tgatttttga tttgggtggt
      601 ggtacttttg acgtctcttt actttcaatt gatgagggtg tttttgaggt taaggctacc
      661 gcaggagaca ctcatttagg tggtgaagat tttgataata ggttggtgaa ccatttagcc
      721 actgaattca aaaggaaaac gaaaaaggac atctctaata atcaaagatc gttaagaaga
      781 ttgagaactg cggcagaaag agctaagaga gcgctttctt cctcatctca aacctcgatc
      841 gagatcgatt ctttatttga aggtatggat ttctacactt cgttaacaag ggcaaggttt
      901 gaagagctat gtgctgattt attcagatcc acattggaac cagtagaaaa ggttcttaaa
      961 gattcgaagc tggacaagtc ccaaattgat gagattgtgt tagtcggtgg atctaccaga
     1021 atcccaaaga ttcagaaatt agtttctgac ttcttcaatg gcaaagagcc taatcgttct
     1081 atcaacccgg atgaggctgt tgcttatggt gcagccgttc aagctgccat tttaaccggc
     1141 gatcaatcaa caaagacaca agatttacta ttattggatg ttgcgccatt gtccctagga
     1201 attgaaactg caggcggcat aatgactaag ctaattccta gaaactcaac gattccaaca
     1261 aagaaatcgg aaaccttctc tacctatgca gataatcaac ctggtgtttt aattcaagtc
     1321 tttgaaggtg aaagaacaag aacaaaggat aataacttac ttggtaaatt cgaattaagt
     1381 ggcattccgc ctgctcccag aggtgtgcct caaattgatg ttacctttga tatcgacgct
     1441 aatggtattc ttaatgtgtc tgctttggaa aagggtactg gtaagagtaa caaaatcacg
     1501 atcactaacg ataaaggtag gctctcgaag gatgatattg ataggatggt ttctgaagct
     1561 gaaaaatata gggctgacga tgaaagggag gcagaacgag ttcaggctaa gaatcagctt
     1621 gaatcgtatg catttacttt gaagaatacc ataaacgaag caagtttcaa agagaaagta
     1681 ggtgaagatg atgcaaagag attagaaaca gcgtctcagg aaaccattga ctggttagat
     1741 gcatcgcagg cagcctctac ggacgaatat aaggatagac aaaaggagtt ggaaggcatt
     1801 gccaatccaa taatgacgaa attttacggt gctggtgccg gcgcaggtcc tggagcgggg
     1861 gaatccggtg gattccccgg atccatgccc aactcgggtg ctacgggagg tggagaagat
     1921 acaggtccaa cagtggaaga ggttgattga ttattcttct atagtgttct atttagctac
     1981 tttttatgtt taacctttta tacgatggcg ggtaatctat ccatgatgac gaaaaatttt
     2041 tttttttttt gtttccgcag cacgcaagaa atctcgaaac aatgatgact cttaagcatg
     2101 aaaaatatca ttttgcgctt taaactagag tgatgttact c
//