From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Turnip mosaic virus cDNA to genomic RNA for CI, 6K, NIa, NIb and
Date: 21 Jul 92 10:08:44 GMT
Distribution: bionet
Checksum: 50851    11

LOCUS       TMVCAP       5102 bp ss-RNA             VRL       21-JUL-1992
DEFINITION  Turnip mosaic virus cDNA to genomic RNA for CI, 6K, NIa, NIb and
            capsid protein.
ACCESSION   D10601 D01090
KEYWORDS    6K protein; CI protein; NIa protein; NIb protein; capsid protein;
            coat protein.
SOURCE      Turnip mosaic virus cDNA to genomic RNA.
  ORGANISM  Turnip mosaic virus
            Viridae; ss-RNA nonenveloped viruses; Rod-shaped ss-RNA viruses.
REFERENCE   1  (sites)
  AUTHORS   Tremblay,M.-., Nicolas,O., Sinha,R.C., Lazure,C. and Laliberte,J.-.
  TITLE     Sequence of the 3'-terminal region of turnip mosaic virus RNA and
            the capsid protein gene
  JOURNAL   J. Gen. Virol. 71, 2769-2772 (1990)
  STANDARD  full staff_review
REFERENCE   2  (bases 1 to 5102)
  AUTHORS   Laliberte,J.-.
  JOURNAL   Unpublished (1992)
  STANDARD  full staff_review
REFERENCE   3  (sites)
  AUTHORS   Nicolas,O., Charbonneau,A. and Laliberte,J.-.
  TITLE     Molecular cloning and expression of the turnip mosaic potyvirus NIa
            protease in Escherichia coli
  JOURNAL   Unpublished (1992)
  STANDARD  full staff_review
COMMENT
            [1]  sites.
            [2]  sites.
FEATURES             Location/Qualifiers
     CDS             <1..4893
                     /product="polyprotein precursor"
                     /codon_start=1
                     /translation="NIIENGVTLDIDVVADFGTKVLPYLDTDNRMLSTTKTSINYGER
                     IQRLGRVGRHKPGHALRIGHTERGLSEVPSCIATEAALKCFTYGLPVITNNVSTSILG
                     NVTVKQARTMSVFEITPFYTSQVVRYDGSMHPQVHALLKRFKLRDSEIVLTKLAIPNR
                     GVNAGSQPVSMHDSVQMLKIGVTLRIPFMCRDIPEKLHLDMWDVVVKFKGDAGFGRLS
                     SSASKVAYTLQTDVNSIQRTVTIIDTLIAEERRKQEYFKTVTSNCVSSSNFSLQSITN
                     AIKSRMMKDHPCENISVLEGAKSQLLEFRNLNSDHSFVTKTDGISRSFMRDYGALEAV
                     NHQSTNEMSKFLQLKGKWNKTLITRDVLVICGVLGGGVWMVVQHFRSKVSEPVTHEAK
                     GKKQRQKLKFRNARDNKMGREVYGDDDTIEHFFGDAYTKKGKSKGRTRGIGHKNRKFI
                     NMYGFDPEDFSAVRFVDPLTGATLDDNPFTDITLVQKHFGDIRMDLLGEDELDSNEIR
                     MNKTIQAYYMNNKTGKALKVDLTPHIPLKVCDLHATIAGFPERENELRQTGKAQPINI
                     DEVPRANNELVPVDHESNSMFRGLRDYNPISNNICHLTNVSDGASNSLYGVGFGPLIL
                     TNRHLFERNNGELIIKSRHGEFVIKNTTQLHLLPIPDRDLLLIRLPKDVPPFPQKLGF
                     RQPEKGERICMVGSNFQTKSITSIVSETSTIMPVENSQFWKHWISTKDGQCGSPMVST
                     KDGKILGLHSLANFQNSINYFAAFPDDFTEKYLHTIEAHEWVKHWKYNTSAISWGSLN
                     IQASQPVSLFKVSKLISDLDSTAVYAQTQQNRWMFEQLTGNLKAIAHCPSQLVTKHTV
                     KGKCQMFDLYLKLHDEAREYFQPMLGQYQKSKLNREAYAKDLLKYATPIEAGNIDCDL
                     FEKTVEIVISDLRGYGFETCNYVTDENDIFEALNMKSAVGALYKGKKKDYFAEFTPEV
                     KEEILKQSCERLFLGKMGVWNGSLKAELRPLEKVEANKTRTFTAAPLDTLLGGKVCVD
                     DFNNQFYDHNLRAPWDVGMTKFYCGWDRLLESLPDGWVYCDADGSQFDSSLSPYLINA
                     VLNIRLGFMEEWDVGEVMLRNLYTEIVYTPISTPDGTLVKKFKGNNSGQPSTVVDNTL
                     MVILAVNYSLKKGGIPSELRDSIIRFFVNGDDLLLSVHPEYEYILDTMADNFRELGLK
                     YTFDSRTREKGDLWFMSHQGHRREGIWIPKLEPERIVSILEWDRSKEPCHRLEAICAA
                     MIESWGYDKLTHEIRKFYAWMIEQAPFSSLAQEGKAPYIAETALRKLYLDKEPAQEDL
                     TQYLQAIFGDYEDGVEACVYHQAGETLDADLTEEQKQAEKEKKEREKAEKERERQKQL
                     AFKKGKDVAQEEGKRDKEVNAGTSGTFSVPRLKSLTSKMRVPRYEKRVALNLDHLILY
                     TPEQTDLSNTRSTRKQFDTWFEGVMADYELTEDKMQIILNGLRVWCIENGTSPNINGM
                     WVMMDGDDQVEFPIKPLIDHAKPTFRQIMAHFSDVAEAYIEKRNQDRPYMPRYGLQRN
                     LTDMSLARYAFDFYEMTSRTPIRAREAHIQMKAAALRGANNNLFGLDGNVGTTVENTE
                     RHTTEDVNRNMHNLLGVQGL"
     mat_peptide     <1..1011
                     /product="CI protein"
                     /codon_start=1
     mat_peptide     1012..1170
                     /product="6K protein"
                     /codon_start=1
     mat_peptide     1171..2475
                     /product="NIa protein"
                     /codon_start=1
     mat_peptide     2476..4026
                     /product="NIb protein"
                     /codon_start=1
     mat_peptide     4027..4890
                     /product="capsid protein"
                     /codon_start=1
BASE COUNT     1614 a   1063 c   1254 g   1171 t
ORIGIN
        1 aacataattg agaatggtgt caccctagac attgatgtgg tcgccgactt tggaacgaaa
       61 gtacttccat atcttgacac agacaacagg atgcttagca caacaaagac aagcattaac
      121 tacggggagc ggattcaaag acttggaaga gttgggaggc acaagccagg tcatgcacta
      181 cgaattggcc acacagagag aggattgagc gaagtcccaa gttgcattgc aacagaagca
      241 gctttgaagt gcttcactta tggactccca gtaatcacca acaacgtatc aacaagcatt
      301 cttggtaatg taacagtgaa gcaggcacga actatgtctg tgtttgagat aacaccattc
      361 tacacaagcc aagtagtgag gtatgatggt tcaatgcacc cacaagtgca tgcactttta
      421 aaaagattca agctcagaga ttctgagatt gttttgacta aactggccat acccaaccga
      481 ggagtaaacg ctggctcaca gccagtgagt atgcacgact cggtgcaaat gttgaagatc
      541 ggcgtgacgt tacgaatccc attcatgtgc cgtgacattc cggagaagct ccatctggac
      601 atgtgggacg ttgttgtgaa atttaaaggt gacgctggct ttgggcgact ctcaagcagc
      661 gcgagcaaag tagcatacac cttgcagaca gatgtcaact ccatacagcg aacagtcact
      721 atcattgata cactaatcgc tgaggagagg agaaagcaag agtacttcaa aacagtcacc
      781 tcaaactgtg tttcttcttc caacttctca ttgcaaagca taacaaatgc cataaaatct
      841 cgcatgatga aagatcaccc atgtgagaac atatcggtgc ttgagggagc gaagtcacaa
      901 ctgcttgaat tcagaaatct caattctgac cactcattcg tcactaaaac tgacggaata
      961 tctcggagct ttatgaggga ctatggagca cttgaggcag ttaaccacca aagcaccaac
     1021 gaaatgagca agttcctgca gcttaaaggc aagtggaata aaacgctaat cacgcgagat
     1081 gtgttggtga tctgcggagt gcttggaggt ggagtatgga tggttgtcca acacttccga
     1141 tcaaaggttt ctgaacccgt aactcatgaa gcaaaaggca agaagcaaag acagaaactg
     1201 aagtttcgca atgcccgaga caataaaatg ggtagggagg tgtacggaga tgacgacacc
     1261 atagagcatt tcttcgggga tgcttacaca aagaaaggga agagcaaagg caggacacgt
     1321 ggtattgggc ataagaacag gaagttcatt aacatgtatg ggtttgatcc tgaggatttc
     1381 tctgcggtta gattcgtaga tccactcaca ggggcaacac tagacgataa cccattcacg
     1441 gacatcaccc ttgtacaaaa acactttggt gacataagaa tggacttact cggagaggac
     1501 gaacttgact caaatgaaat acggatgaac aagacaattc aggcctacta catgaacaat
     1561 aaaacaggta aggctttgaa ggtggatctg acaccacaca tacctctcaa ggtgtgtgat
     1621 cttcacgcaa ccattgctgg attcccagag cgagaaaacg aactgaggca gactggaaag
     1681 gctcagccca tcaacataga cgaagtgcca agagctaaca acgaactcgt cccagtggac
     1741 cacgagagta actccatgtt cagagggttg cgcgattaca acccaatatc aaacaacatt
     1801 tgtcatctca caaatgtttc agatggagca tcaaactcgt tatatggagt cggtttcggg
     1861 ccactcatat tgacgaaccg acacctcttt gagcggaaca acggtgaact cataataaaa
     1921 tcacgacatg gtgaattcgt gattaaaaac acaactcagc tacatttgct accgattccg
     1981 gacagagatc ttttgttaat ccggttacca aaggacgtcc caccctttcc acagaaacta
     2041 ggtttcaggc aacctgagaa gggtgaacga atttgtatgg tggggtccaa tttccaaacc
     2101 aagagcataa cgagtatagt ctctgagact agcacaataa tgccagtgga gaacagtcag
     2161 ttttggaaac actggattag cactaaagac ggccaatgtg gaagtccaat ggtgagcaca
     2221 aaggacggga aaatactcgg attacacagt ctagcgaatt tccagaactc catcaattac
     2281 tttgctgctt tcccagatga tttcaccgag aagtatcttc acaccattga agcacacgag
     2341 tgggtcaagc actggaaata caacactagc gccatcagtt ggggttcttt gaacatacaa
     2401 gcatctcaac cggtcagctt gttcaaagtg agcaagctaa tctcagacct cgatagcacg
     2461 gcagtctacg cacaaaccca gcagaatcgg tggatgttcg agcagctcac tggaaacctg
     2521 aaagctatag cacattgccc tagccagctt gtgacaaagc acacagttaa aggaaaatgt
     2581 cagatgtttg acttgtatct caagttacat gacgaagcac gagagtattt ccaaccgatg
     2641 ctgggccagt atcagaagag taaactcaat cgagaagcat atgcaaaaga tcttctgaaa
     2701 tatgcaacgc caatcgaagc aggaaacatt gactgtgatc tgtttgaaaa gacagttgaa
     2761 atagtcatat cagatctgcg aggttatggt ttcgaaacat gcaattatgt cactgatgag
     2821 aatgacatat tcgaagctct taacatgaaa tccgcagttg gagcgttgta taaaggaaag
     2881 aagaaggatt actttgctga gttcacaccc gaggtgaaag aagaaatact gaaacaaagt
     2941 tgcgaacgac tcttcttggg gaagatggga gtgtggaatg gctcgttgaa ggcagagttg
     3001 cgaccactag aaaaagtgga agccaacaaa acacgcacgt ttacagccgc accactagac
     3061 acactgttgg gtggaaaagt ttgtgtggat gatttcaaca accagttcta tgatcacaac
     3121 cttagagctc cttgggacgt tggcatgaca aagttttatt gtggttggga tcgattgttg
     3181 gaatcattgc cagatggttg ggtgtattgc gatgctgatg gctcacagtt tgacagctcg
     3241 ttatcgccat acttgatcaa tgcagtactc aacattcgct taggcttcat ggaagagtgg
     3301 gatgtagggg aggtaatgct gagaaatttg tacaccgaaa tcgtgtatac ccccatttct
     3361 acaccagatg gtacactcgt caagaaattc aaaggaaaca atagcggaca gccatcgact
     3421 gttgtagaca acacactcat ggtcatattg gcagtcaact attcactcaa gaaaggcgga
     3481 attccaagtg agttgcgcga cagcatcatc agattcttcg tcaacggaga cgatttactg
     3541 ctaagcgtac acccagagta tgagtatatc cttgacacta tggcagacaa ctttcgagaa
     3601 ctgggtctga agtacacttt cgactcgagg actagggaaa aaggagacct ctggtttatg
     3661 tcgcatcagg ggcacagaag agagggaatt tggattccta agcttgaacc agagcgaata
     3721 gtatcaattc tagaatggga tcggtcgaaa gaaccatgcc atcgattgga ggcaatctgc
     3781 gcagcgatga ttgagtcgtg gggatacgac aagttaactc acgagatacg caagttttac
     3841 gcgtggatga ttgaacaagc tccatttagc tccctagcac aagaagggaa agctccttat
     3901 atagcggaaa cagcgctgag gaagctctac cttgataagg aaccagctca agaagatctc
     3961 acccagtatt tgcaggcaat ctttggggat tatgaagatg gtgttgaggc ttgtgtctat
     4021 caccaggcag gtgaaacgct tgacgcagac ttgacagaag agcaaaagca ggcagagaag
     4081 gagaagaaag agagagagaa ggcagaaaag gagcgagaga ggcagaaaca gttggcattc
     4141 aagaaaggca aggatgtcgc acaagaagag ggaaaacgcg acaaggaagt aaacgctgga
     4201 acctctggaa ctttcagtgt acccagactc aagagtctga caagcaagat gcgcgtgcca
     4261 agatatgaga aaagagtggc tctaaacctc gatcatctaa tcctatacac gccggagcag
     4321 acggatctat ccaacacacg ttcaacgcga aagcagtttg acacatggtt tgaaggtgta
     4381 atggctgatt acgaattgac ggaggacaaa atgcaaatca ttctcaatgg tttgagggtc
     4441 tggtgcattg agaacggaac ctccccgaac ataaacggaa tgtgggtgat gatggacggc
     4501 gatgatcagg tggaattccc gatcaaaccg ctcattgacc acgccaaacc cacatttagg
     4561 cagataatgg cccatttcag tgacgtagct gaagcgtaca ttgaaaagcg taaccaagac
     4621 cgaccataca tgccacgata tggtcttcag cgcaatttaa ccgacatgag cttagctcga
     4681 tacgcatttg atttctatga aatgacttct aggactccaa tacgtgcgag agaggcacac
     4741 atccagatga aagcggcagc actgcgtggc gcaaataata atttgttcgg cttggatgga
     4801 aacgttggta caacggtaga gaacacggag aggcatacga ccgaggacgt taatcggaac
     4861 atgcataact tgctgggcgt tcaggggtta tgaagttgta tgctggtaga ctataagtat
     4921 ttaagtttac tcgttagtat tctcgcttat gggaaatatg taagtttgtt aaagcagcca
     4981 gtgtgacttt gtcatgtgtg ttgttgttac tttctgtatt ttcgccgaac attttattgg
     5041 tgttagcgca tgtggtgagg atcgtcctcg attgccttaa catctgatag gatgcaaggg
     5101 ac
//