From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: S.pneumoniae (63915) ponA gene for penicillin-binding protein 1a
Date: 28 Dec 92 21:46:17 GMT
Distribution: bionet
Checksum: 05054 5

LOCUS       STRPONA7     2157 bp ds-DNA             BCT       02-DEC-1992
DEFINITION  S.pneumoniae (63915) ponA gene for penicillin-binding protein 1a
ACCESSION   X67872
KEYWORDS    penicillin-binding protein 1a; ponA gene.
SOURCE      Streptococcus pneumoniae DNA.
  ORGANISM  Streptococcus pneumoniae
            Prokaryotae; Firmicutes; Firmibacteria; Gram-positive cocci;
            Streptococcaceae.
REFERENCE   1  (bases 1 to 2157)
  AUTHORS   Martin,C., Sibold,C. and Hakenbeck,R.
  TITLE     Relatedness of penicillin-binding protein 1a genes from different
            clones of penicillin-resistant Streptococcus pneumoniae isolated in
            South Africa and Spain
  JOURNAL   EMBO J. 11, 3831-3836 (1992)
  STANDARD  full automatic
COMMENT     *source: strain=63915; *source: cell_line=plant cultivar; *source:
            clone=pcMK1-pcMK16;
               From EMBL    entry SPPONA7;  dated 21-OCT-1992.
FEATURES             Location/Qualifiers
     CDS             1..>2157
                     /gene="ponA"
                     /product="penicillin-binding protein1a"
                     /codon_start=1
                     /translation="MNKPTILRLIKYLSISFLSLVIAAIVLGGGVFFYYVSKAPSLSE
                     SKLVATTSSKIYDNKNQLIADLGSERRVNAQANDIPTDLVKAIVSIEDHRFFDHRGID
                     TIRILGAFLRNLQSNSLQGGSTLTQQLIKLTYFSTSTSDQTISRKAQEAWLAIQLEQK
                     ATKQEILTYYINKVYMSNGNYGMQTAAQNYYGKDLNNLSLPQLALLAGMPQAPNQYDP
                     YSHPEAAQDRRNLVLSEMKNQGYISAEQYEKAVNTPITDGLQSLKSASNYPAYMDNYL
                     KEVINQVEEETGYNLLTTGMDVYTNVDQEAQKHLWDIYNTDEYVAYPDDELQVASTIV
                     DVSNGKVIAQLGARHQSSNVSFGINQAVETNRDWGSTMKPITDYAPALEYGIYDSTAT
                     IVHDKPYNYPGTNTPVYNWDRGYFGNITLQYALQQSRNVPAVETLNKVGLNRAKTFLN
                     GLGIDYPSIHYSNAISSNTTESDKKYGASSEKMAAAYAAFANGGTYYKPMYIHKVVFS
                     DGSEKEFSNVGTRAIKETTAYMMTDMMKTVLSYGTGRNAYLAWLPQAGKTGTSNYTDE
                     EIENHIKTSQFVAPDELFAGYTRKYSMAVWTGYSNRLTPLVGNGLTVAAKVYRSMMTY
                     LSEGSNPEDWNIPEGLYRNGEFVFKNGARSTWNSPAPQQPPSTESSSSSSDSSTSQSS
                     STTPSTNNSTTTNPNNNTQQSNTTPDQQNQNPQPAQP"
BASE COUNT      699 a    521 c    407 g    530 t
ORIGIN      
        1 atgaacaaac caacgattct gcgcctaatc aagtatctga gcattagctt cttaagcttg
       61 gttatcgcag ccattgtctt aggcggagga gtttttttct actacgttag caaggctcct
      121 agcctatccg agagtaaact agttgcaaca acttctagta aaatctacga caataaaaat
      181 caactcattg ctgacttggg ttctgaacgc cgcgtcaatg cccaagctaa tgatattccc
      241 acagatttgg ttaaggcaat cgtttctatc gaagaccatc gcttcttcga ccacaggggg
      301 attgatacca tccgtatcct gggagctttc ttgcgcaatc tgcaaagcaa ttccctccaa
      361 ggtggatcaa ctctcactca acagttgatt aagttgactt acttttcaac ttcgacttcc
      421 gaccagacta tttctcgtaa ggctcaggaa gcttggttag cgattcagtt agaacaaaaa
      481 gcaaccaagc aagaaatctt gacctactat ataaataagg tctacatgtc taatgggaac
      541 tatggaatgc agacagcagc tcaaaactac tatggtaaag acctcaataa tttaagttta
      601 cctcagttag ccttgctggc tggaatgcct caggcaccaa accaatatga cccctattca
      661 catccagaag cagcccaaga ccgccgaaac ttggtcttat ctgaaatgaa aaatcaaggc
      721 tacatctctg ctgaacagta tgaaaaagca gtcaatacac caattactga tggactacaa
      781 agtctcaaat cagcaagtaa ttaccctgct tacatggata attacctcaa ggaagtcatc
      841 aatcaagttg aagaagaaac aggctataac ctgctcacaa ctgggatgga tgtctacaca
      901 aatgtagacc aagaagctca aaaacatctg tgggatattt acaatacaga cgaatacgtt
      961 gcctatccag acgatgaatt gcaagtcgct tctaccattg ttgatgtttc taacggtaaa
     1021 gtcattgccc agctaggagc acgccatcag tcaagtaatg tttccttcgg aattaaccaa
     1081 gcagtagaaa caaaccgcga ctggggatca actatgaaac cgatcacaga ctatgctcct
     1141 gccttggagt acggtatcta cgattcaact gctactatcg ttcacgataa gccctataac
     1201 taccctggga caaatacccc tgtttataac tgggataggg gctactttgg caacatcacc
     1261 ttgcaatacg ccctgcaaca atcgcgaaac gtcccagccg tggaaactct aaacaaggtc
     1321 ggactcaacc gcgccaagac tttcctaaat ggtctcggaa tcgactaccc aagtattcac
     1381 tactcaaatg ccatttcaag taacacaacc gaatcagaca aaaaatatgg agcaagtagt
     1441 gaaaagatgg ctgctgctta cgctgccttt gcaaatggtg gaacttacta taaaccaatg
     1501 tatatccata aagtcgtctt tagtgatggg agtgaaaaag agttctctaa tgtcggaact
     1561 cgtgccatca aggaaacgac agcctatatg atgaccgaca tgatgaaaac agtcttgagt
     1621 tatggaactg gacgaaatgc ctatcttgct tggctccctc aggctggtaa aacaggaacc
     1681 tctaactata cagacgagga aattgaaaac cacatcaaga cctctcaatt tgtagcacct
     1741 gatgaactat ttgctggcta tacgcgtaaa tattcaatgg ctgtatggac aggctattct
     1801 aaccgtctga caccacttgt aggcaatggc cttacggtcg ctgccaaagt ttaccgctct
     1861 atgatgacct atctgtctga aggaagcaat ccagaggatt ggaatatacc agaggggctc
     1921 tacagaaatg gagaattcgt atttaaaaat ggtgctcgtt ctacgtggaa ctcacctgct
     1981 ccacaacaac ccccatcaac tgaaagttca agctcatcat cagatagttc aacttcacag
     2041 tctagctcaa ccactccaag cacaaataat agtacgacta ccaatcctaa caataatacg
     2101 caacaatcaa atacaacccc tgatcaacaa aatcagaatc ctcaaccagc acaacca
//