From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Z. mobilis invA gene encoding intracellular invertase E1.
Date: 24 Jul 92 10:11:07 GMT
Distribution: bionet
Checksum: 28340     5

LOCUS       ZMOINVA      2316 bp ds-DNA             BCT       24-JUL-1992
DEFINITION  Z. mobilis invA gene encoding intracellular invertase E1.
ACCESSION   D10465 D01071
KEYWORDS    invertase.
SOURCE      Zymomonas mobilis (strain Z6C) DNA, clone pUSH11.
  ORGANISM  Zymomonas mobilis
            Prokaryotae; Facultatively anaerobic gram-negative rods.
REFERENCE   1  (bases 1 to 2316)
  AUTHORS   Yanase,H., Fukushi,H., Ueda,N., Maeda,Y., Toyoda,A. and Tonomura,K.
  TITLE     Cloning, sequencing, and characterization of the intracellular in
            envertase gene from Zymomonas mobilis
  JOURNAL   Agric. Biol. Chem. 55, 1383-1390 (1991)
  STANDARD  full staff_review
COMMENT     These data kindly submitted in computer readable form by:
            Hideshi Yanase
            Department of Biotechnology
            Faculty of Engineering, Tottori University
            4-101 Koyamacho-minami
            Tottori 680
            Japan
            Phone:  0857-23-0321
            Fax:    0857-31-0881
FEATURES             Location/Qualifiers
     -35_signal      252..264
     -10_signal      273..281
     RBS             360..365
     CDS             371..1909
                     /EC_number="3.2.1.26"
                     /product="invertase"
                     /gene="invA"
                     /codon_start=1
                     /translation="MESPSYKNLIKAEDAQKKAGKRLVSSEWYPGFHVTPLTGWMNDP
                     NGLIFFKGEYHLFYQYYPFAPVWGPMHWGHAKSRDLVHWETLPVALAPGDSFDRDGCF
                     SGCAVDNNGILTLIYTGHIVLSNDSPDAIREVQCMATSIDGIHFQKEGIILEKPPMPQ
                     VAHFRDPRVWKENDRWFMVVGYRTDDEKHQGIGHVALYRSENLKDWIFVKTLLGDNSQ
                     LPLGKRAFMWECPDFFSLGNRSVLMFSPQGLKASGYKNRNLFQNGYILGKWQAPQFTP
                     ETSFQELDYGHDFYAAQRFEDKDGRQILIAWFDMWENQKPSQRDGWAGCMTLPRKLDL
                     IDNKIVMTPVREMEILRQSEKIESVVTLSDAEHPFTIDSPLQEIELIFDLEKSSAYQA
                     GLALRCNDKGQETLLYIDRSQNRIILDRNRSGQNVKGIRSCPLPNTSKVRLHIFLDRS
                     SIEIFVGDDQTQGLYSISSRIFPDKDSLKGRLFAIEGYAVFDSFKRWTLQDANLAAFL
                     SDAC"
BASE COUNT      672 a    470 c    516 g    658 t
ORIGIN
        1 aagcttgcat gctcgatgaa gacgatttat aatttttatt gaaaaataaa tttcggcgaa
       61 aggctgcaaa gggaaacgct tttgtagcct tttgtctatc aaggctaaaa atggttgggg
      121 cggcaggatt cgaacctgcg catgacggga tcaaaacccg ttgccttacc gcttggctac
      181 gccccaatag actgcattaa gagtgcagtc tatttttgga gagccgtttt atagcaagta
      241 aatttttttt gaaaagcctt gcgaccaaaa aatatagaaa aaagacagct tcatctctcc
      301 caaaaggaaa aatttttgtc tttggtcgtt actctttata attcttccat ccgaataaaa
      361 ggcagcgaca atggaatccc cctcttataa aaatctaatc aaagccgagg atgcacagaa
      421 aaaagctgga aaacggcttg tatcttctga atggtatcct ggatttcatg taacgccctt
      481 aactggttgg atgaatgacc ctaacggcct gatttttttc aagggggaat atcatctttt
      541 ttaccaatat tatccttttg cgcccgtctg ggggccgatg cattggggac atgcgaaaag
      601 ccgtgattta gttcattggg aaacactccc tgttgccctt gctccagggg attcatttga
      661 tcgcgacggc tgtttctcag gatgcgctgt tgataataat ggtattttga ctctgatcta
      721 tacggggcat atcgttttat ccaacgatag ccccgatgcc attcgtgaag tgcagtgtat
      781 ggcgacaagc atagacggca tccattttca aaaagaaggc atcatccttg aaaaaccacc
      841 catgccacag gttgcccatt tcagagaccc tcgcgtttgg aaggagaatg accgctggtt
      901 tatggtggtg ggctatcgga cagatgatga gaaacatcaa ggaatcggac atgtcgctct
      961 atacagaagt gaaaatctaa aagactggat ttttgtcaaa acactactgg gcgataattc
     1021 tcaattaccg ctgggaaaac gggcttttat gtgggaatgt cctgatttct tctcattagg
     1081 caatcgttct gttttgatgt tttctccgca aggcttgaag gcaagcggtt acaaaaaccg
     1141 gaatctattt caaaacggct atatccttgg aaaatggcaa gcacctcaat ttacgccgga
     1201 aacatccttc caagaactag actatggtca tgatttctat gcagcacaac gttttgaaga
     1261 caaagatggt cgtcaaatat taattgcatg gtttgatatg tgggaaaatc aaaagcccag
     1321 ccaacgagat ggatgggctg gctgtatgac gttgccccgc aagcttgatt taattgataa
     1381 taaaattgtg atgacgcctg ttagggaaat ggagatttta cggcaatcgg aaaaaattga
     1441 atctgtcgta acgctttcgg atgccgagca tccctttacg atagattctc ccctgcaaga
     1501 aatagagctg atctttgact tggaaaaaag cagtgcttat caagctggtt tggctttgcg
     1561 ctgtaatgac aaagggcaag aaacgcttct ttatatcgat agaagccaga atcgtatcat
     1621 tcttgaccgg aatcgctcgg ggcaaaatgt aaaagggatt agaagctgtc cgcttccgaa
     1681 cacctctaaa gtcagactgc atatattcct tgaccgatca tctatagaaa tttttgtcgg
     1741 cgatgatcag acccaaggcc tctacagcat aagtagtcga atattccccg ataaggacag
     1801 ccttaaaggc cgtttattcg ctatcgaagg ctatgccgtt tttgattctt ttaagagatg
     1861 gacattacag gatgctaatc tagccgcttt tttaagcgat gcctgttaaa aatggttggg
     1921 gcggcaggat tcgaacctgc gcatgacggg atcaaaaccc gttgccttac cgcttgctac
     1981 gccccaatag gcttctcggt cgagaatgat cttctttata acgactgaaa cgggcttgaa
     2041 aagtcctgat ttctaaaaat ttttcttttc tcgacctcaa ttcgggcaat tcctccgaaa
     2101 ggatctcgat tatggctatt ctgataacag gcattgcagg ctttattggt agttttgctg
     2161 caaaatccct tttagaaaga ggtgaagaaa taatcggaat agataatcta aatgattatt
     2221 atgatcccga gttaaagaaa aaacgtttgg cagaattaaa aaagctcaaa aacggcaagc
     2281 tgcatttttt tcctatcgat ttttcagacg gagctc
//