From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: D.pseudoobscura Adh gene for alcohol dehydrogenase
Date: 28 Dec 92 21:27:07 GMT
Distribution: bionet
Checksum: 09280 8

LOCUS       DROADHGN37   3468 bp ds-DNA             INV       01-DEC-1992
DEFINITION  D.pseudoobscura Adh gene for alcohol dehydrogenase
ACCESSION   X62217
KEYWORDS    ADH gene; alcohol dehydrogenase.
SOURCE      Drosophila pseudoobscura DNA.
  ORGANISM  Drosophila pseudoobscura
            Eukaryota; Animalia; Metazoa; Arthropoda; Uniramia; Insecta;
            Pterygota; Neoptera; Holometabola; Diptera; Brachycera;
            Cyclorrhapha; Schizophora; Drosophiloidea; Drosophilidae.
REFERENCE   1  (bases 1 to 3468)
  AUTHORS   Schaeffer,S.W. and Miller,E.L.
  TITLE     Estimates of gene flow in Drosophila pseudoobscura determined from
            nucleotide sequence analysis of the alcohol dehydrogenase region
  JOURNAL   Genetics 132, 471-480 (1992)
  STANDARD  full automatic
COMMENT     *source: strain=GC4; *source: isolate=Gregory Canyon, Colorado;
            *source: chromosome=IV section 88;
               From EMBL    entry DPADHGN37;  dated 16-OCT-1992.
FEATURES             Location/Qualifiers
     mRNA            join(40..119,913..1018,1082..1486,1547..1961)
                     /note="adult mRNA (Adh)"
     mRNA            join(880..1018,1082..1486,1547..1961)
                     /note="larval mRNA (Adh)"
     promoter        8..14
                     /note="adult promoter (Adh)"
     prim_transcript 40..1961
                     /note="adult primary transcript (Adh)"
     exon            40..119
                     /note="adult exon 1a"
     intron          120..912
                     /note="adult intron"
     promoter        844..851
                     /note="larval promoter (Adh)"
     prim_transcript 880..1961
                     /note="larval primary transcript (Adh)"
     exon            880..1018
                     /note="larval exon 1"
     exon            913..1018
                     /note="adult exon 1b"
     intron          1019..1081
                     /number=1
     exon            1082..1486
                     /number=2
     intron          1487..1546
                     /number=2
     exon            1547..1961
                     /number=3
     polyA_signal    1938..1943
     polyA_site      1961
     CDS             join(926..1018,1082..1486,1547..1813)
                     /EC_number="1.1.1.1"
                     /gene="Adh"
                     /product="alcoholdehydrogenase"
                     /codon_start=1
                     /translation="MSLTNKNVVFVAGLGGIGLDTSRELVKRNLKNLVILDRIDNPAA
                     IAELKAINPKVTITFYPYDVTVPVAETTKLLKTIFAQVKTIDVLINGAGILDDHQIER
                     TIAVNYTGLVNTTTAILDFWDKRKGGPGGIICNIGSVTGFNAIYQVPVYSGSKAAVVN
                     FTSSLAKLAPITGVTAYTVNPGITKTTLVHKFNSWLDVEPRVAEKLLEHPTQTSQQCA
                     ENFVKAIELNKNGAIWKLDLGTLEPITWTQHWDSGI"
BASE COUNT      965 a    794 c    801 g    908 t
ORIGIN      
        1 cgtagtgtat ttaaggcact gcacatgtcg aggaccacga ttattgtcta cagtgcagtc
       61 agtagttgtc aggtgcagca ggcggaccag catttcctta aatctactca atcgatcaag
      121 taagtaactg taatccaagt acagtcggcc catcccactc ggttctgccg tagctcaaga
      181 aatttcttga tagcgcagtt ccagttgtaa aacgtcgttt aaaaaaacat ctcgttggag
      241 cttgggttga aaaacgttcg agttgggcgt agaaaagttc aaatataaaa tatcaaaaaa
      301 gagaaaatat cttcccaaag cagctgtcca ttttttgtta aagtgataga tcaaaaattc
      361 gtatgacgta tttcccaagt gctctttatg caaataattt cctcaattct aattctacaa
      421 gaagcacttt tggctcttgt ccagaaaaaa caatttgttt acacttttcg gttgataaca
      481 gaggcggacc gacggctaag taacataaca gtcgcctgtt aagtaatgcc ctcactcgct
      541 gttagatggc cgcgcgtgtc gactccgata gaccagcaga cacgcagtag ttttcctcaa
      601 gaacctgaac tctaaacata gacataattt acgatacctt gagactcaaa taaaaataca
      661 agtatgagtg gtattctatt cctgattaga ctcgcacaca cacatacgag tattaacata
      721 aatttgccgg caaataacca aaacaaaacg aacgcaaacg atccgaacac gtaatgcgag
      781 agataagaaa cagaaaagct cgacgtgaaa gaagcttctg gcggaagctc ttgccgaact
      841 ccctataaat acgagacaga aaaccagaat aaatataata acaaactgtt gaattgtccc
      901 atcgattccc agatcaaaag acagaatgtc tctcaccaac aagaacgtcg ttttcgtggc
      961 cggtctgggc ggcattggcc tggacaccag ccgggagttg gtcaagcgta atctgaaggt
     1021 aagagagaac gaattccatg gaatctatgg aatcctaaat ttaaaaattc atttatttta
     1081 gaacctggtc atcctggatc gcattgacaa tccggctgcc attgccgaac tgaaggcaat
     1141 caatcccaag gtgaccatca ccttctatcc ctacgatgtg actgtgcccg tcgctgagac
     1201 caccaagctc ctgaagacca tctttgccca ggtgaagaca atcgatgtcc tgatcaacgg
     1261 tgctggcatc ctggacgatc atcagatcga gcgcaccatt gccgttaact acacgggcct
     1321 ggtcaacacc accacagcca ttctggactt ctgggacaag cgcaagggcg gcccaggcgg
     1381 catcatttgc aacattggct ccgtcaccgg tttcaatgcc atctaccagg tgcccgttta
     1441 ctccggctcc aaggcggcgg tggttaactt tacctcctcc ctggcggtaa gtaatccaac
     1501 gtttttccta ctccccaaaa ctaaatattt ctttgaaatt ccatagaaac tggctcccat
     1561 tactggtgtc actgcttaca ctgtcaatcc tggcatcacg aagaccactc tggtccacaa
     1621 attcaactcg tggctggatg tggagccccg tgtggcggag aagctgctcg agcatcccac
     1681 ccagacctct cagcagtgcg ccgagaactt tgtgaaggcc attgagctga acaagaacgg
     1741 tgccatctgg aagttggatt tgggcacctt ggagcccatc acatggaccc agcactggga
     1801 ctcgggcatc taaaccatcc cagagactct atgggacatg gcgttttagc ttttagtttc
     1861 ttttttccac tcaattgtta cgtatataca tacacatatg gcaataaggc tgatttgaac
     1921 cccgttttga atatgataat acaaaattat atttgagaaa tttcaacaaa atcgataaga
     1981 gctatagctc tcttttaatt aaaaaaaact agataaaaag agcaatggtc agtggtggtc
     2041 agtggtggtc agtggtcgtt gtggcctcta tcgatttcca cacaaaaact ttactgttta
     2101 gtaatagaaa agaacttgag aaaggcagcc aaaagaatgt acgatctgac gggtaagcat
     2161 gtctgctatg tggctgactg cggtggcatt gcactggaga ccagcaaggt tctcatgacc
     2221 aagaatatag cggtgagtgg tagagtggtg tagagagtgg agagtggttc tggttcttaa
     2281 acaagtctct agtctctagt ctcttggctg ctgctggtag ctgtcgaaac taattaagtt
     2341 aatgactttt tcatgttttg tttttgtccg ctgacttttg ctgttcaaag ctacaattaa
     2401 gtttatgttt tgagtttgat ctacttttgc ttaagggaag tactttcgat tacggattgc
     2461 tggcatataa aaatagatac caaggaatgt gcatcatatt gattctctct tgattctttg
     2521 cccagaaact ggccatcctt cagagcgtgg aaaacccccc ggccatcgct cagttacaat
     2581 cgattaagca cagcacacag atcttcttct ggaccttcga tgtgaccatg gcccgcgagg
     2641 agatgaagaa gtacttcgat gaggtcatgg tccagatgga ctacatcgat gtgctgatca
     2701 atggggccac cctgtgcgac gagcggaaca ttgatgccac catcaatacg aatctgaccg
     2761 gcatgatgaa caccgtggcc actgtgctgc cctatatgga ccgtaagatg ggcggttcgg
     2821 gtggactgat cgtgaatgtc acctccgtca taggactgga tccatcgccg gtcttttgtg
     2881 cgtacagtgc ctcaaagttt ggtgtgattg ggttcacccg aagtctagcg gtgagtgggc
     2941 gagatcttag cttcaaaatg ctctctaatg tcttccattc ttccgcagga tcccctgtat
     3001 tacacccaaa atggtgtggc tgtcatggcc gtctgctgtg gtcccaccaa agtgtttgtc
     3061 gatcgggaac tgactgcctt cctggaatac ggccaaacct ttgccgatcg cctgcgtcgt
     3121 gccccctgcc aatcgacggc cgtctgcggc cagaatatag taaatgccat cgagagatcg
     3181 gagaacggac agatctggat tgccgacaag ggcggcctgg agtcggtggc ccttcactgg
     3241 tattggcata tggccgatca gtttgtgaac tacatgcaga gcactgacga tgaggatcag
     3301 gagttttttt tgggccagcg atgaggcatg ccttgatcca cggaataaaa catcaaatgt
     3361 gtcacaaaaa gtaggcaact tgtttgtggg tctggaaata gatttgaaat attttgaggt
     3421 catatgaata atgacataaa tgagggtgcc tgaaagtatg ccaagttt
//