From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: D.pseudoobscura Adh gene for alcohol dehydrogenase
Date: 28 Dec 92 21:27:24 GMT
Distribution: bionet
Checksum: 51463 8

LOCUS       DROADHGN49   3494 bp ds-DNA             INV       01-DEC-1992
DEFINITION  D.pseudoobscura Adh gene for alcohol dehydrogenase
ACCESSION   X62229
KEYWORDS    ADH gene; alcohol dehydrogenase.
SOURCE      Drosophila pseudoobscura DNA.
  ORGANISM  Drosophila pseudoobscura
            Eukaryota; Animalia; Metazoa; Arthropoda; Uniramia; Insecta;
            Pterygota; Neoptera; Holometabola; Diptera; Brachycera;
            Cyclorrhapha; Schizophora; Drosophiloidea; Drosophilidae.
REFERENCE   1  (bases 1 to 3494)
  AUTHORS   Schaeffer,S.W. and Miller,E.L.
  TITLE     Estimates of gene flow in Drosophila pseudoobscura determined from
            nucleotide sequence analysis of the alcohol dehydrogenase region
  JOURNAL   Genetics 132, 471-480 (1992)
  STANDARD  full automatic
COMMENT     *source: strain=OREG4; *source: isolate=Brookings, Oregon; *source:
            chromosome=IV section 88;
               From EMBL    entry DPADHGN49;  dated 16-OCT-1992.
FEATURES             Location/Qualifiers
     mRNA            join(40..119,915..1020,1084..1488,1551..1984)
                     /note="adult mRNA (Adh)"
     mRNA            join(882..1020,1084..1488,1551..1984)
                     /note="larval mRNA (Adh)"
     promoter        8..14
                     /note="adult promoter (Adh)"
     prim_transcript 40..1984
                     /note="adult primary transcript (Adh)"
     exon            40..119
                     /note="adult exon 1a"
     intron          120..914
                     /note="adult intron"
     promoter        846..853
                     /note="larval promoter (Adh)"
     prim_transcript 882..1984
                     /note="larval primary transcript (Adh)"
     exon            882..1020
                     /note="larval exon 1"
     exon            915..1020
                     /note="adult exon 1b"
     intron          1021..1083
                     /number=1
     exon            1084..1488
                     /number=2
     intron          1489..1550
                     /number=2
     exon            1551..1984
                     /number=3
     polyA_signal    1961..1966
     polyA_site      1984
     CDS             join(928..1020,1084..1488,1551..1817)
                     /EC_number="1.1.1.1"
                     /gene="Adh"
                     /product="alcoholdehydrogenase"
                     /codon_start=1
                     /translation="MSLTNKNVVFVAGLGGIGLDTSRELVKRNLKNLVILDRIDNPAA
                     IAELKAINPKVTITFYPYDVTVPVAETTKLLKTIFAQVKTIDVLINGAGILDDHQIER
                     TIAVNYTGLVNTTTAILDFWDKRKGGPGGIICNIGSVTGFNAIYQVPVYSGSKAAVVN
                     FTSSLAKLAPITGVTAYTVNPGITKTTLVHKFNSWLDVEPRVAEKLLEHPTQTSQQCA
                     ENFVKAIELNKNGAIWKLDLGTLEPITWTQHWDSGI"
BASE COUNT      973 a    788 c    808 g    925 t
ORIGIN      
        1 cgtagtgtat ttaaggcact gcacatgtcg aggaccacga ttattgtcta cagtgcagtc
       61 agtagttgtc aggtgcagca ggcggaccag catttcttta aatctactca atcgatcaag
      121 taagtaactg taatccaagt acagtcggcc catcccactc ggttctgcag taactcaaga
      181 aatttcttga tagcgcagtt ccagttgtag aacgtcgctt aaaaaaacat ctctttggag
      241 cttgggttga aaaacgttcg agttgggcgt agaaaggttc aaatataaaa tatcaaataa
      301 gagaaaatat tctcccaaag cagctgtcca ttttttgtta aagtgataga tcaaaaattc
      361 gtatgacgta tttgccaagt gctctttatg caaataattt cctcaattct aattctacaa
      421 gaagcacttt tggctcttgt ccagaaaaaa caatttgttt acacttttcg gttgataaca
      481 gaggcggacc gacggctaag taacataaca gtcgcctgtt aagtaatgcc ctcactctct
      541 gttagatggc cgcgcgtgtc gactccgata gaccagcaga cacgcagtag ttttcctcaa
      601 gaacctgaac tctaaacata gacataattt acgatacctt gagactcaaa taaaaataca
      661 tgtatgagtg gtattctatt cctgattaga ctcgtacaca cacacgtacg agtattaaca
      721 taaatttgcc ggcaaataac caaaacaaaa cgaacgcaaa cgatccgaac acgtaatgcg
      781 agagataaga aacagaaaag ctcgacgtga aagaagcttc tggcggaagc tcttgccgaa
      841 ctccctataa atacgagaca gaaaaccaga ataaatataa taacaaactg ttgaattgtc
      901 ccatcgattc ccagatcaaa agacagaatg tctctcacca acaagaacgt cgttttcgtg
      961 gccggtctgg gcggcattgg cctggacacc agccgggagt tggtcaagcg taatctgaag
     1021 gtaagagaga acgaattcca tggaatctat ggaatccgaa atttaaaaat taatttattt
     1081 tagaacctgg tcatcctgga tcgcattgac aatccggctg ccattgccga gctgaaggca
     1141 atcaatccca aggtgaccat caccttctat ccctacgatg tgactgtgcc cgtcgctgag
     1201 accaccaagc tcctgaagac catctttgcc caggtgaaga caatcgatgt cctgatcaac
     1261 ggtgctggca tcctggacga tcatcagatc gagcgcacca ttgccgttaa ctacacgggc
     1321 ctggtcaaca ccaccacagc cattctggac ttctgggaca agcgcaaggg cggcccaggc
     1381 ggcatcattt gcaacattgg ctccgtcacc ggtttcaatg ccatctacca ggtgcccgtt
     1441 tactccggct ccaaggcggc ggtggttaac tttacctcct ccctggcggt aagcaatcca
     1501 acgtttttcc tactttttcc aaaactaaat atttctttga aatcccatag aaactggctc
     1561 ccattactgg tgtcactgct tacactgtca atcctggcat cactaagacc actctggtcc
     1621 acaaattcaa ctcgtggctg gatgtggagc cccgtgtggc ggagaagctg ctcgagcatc
     1681 ccacccagac ctctcagcag tgcgccgaga actttgtgaa ggccattgag ctgaacaaga
     1741 acggtgccat ctggaagttg gatctgggca ccttggagcc catcacatgg acccagcact
     1801 gggactcggg catctaaacc atcccagaga ctctatggga catggcgttt tagcttttag
     1861 tttctttttt tccactcaat tgttacgtat atacatacac atatggcaat aaggctgatt
     1921 tgattctctt ttatggaagg aaccccgttt taaatatgat aatacaaaat tatatttgag
     1981 aaatttcaac aaaatcgata agagctatag ctctctttta attaaaaaaa actagataaa
     2041 aagagcaatg gtcagtggtg gtcagtggtc gttgtggcct ctatcgattt ccacacaaaa
     2101 actttactgt ttagtaatag aaaagaactt gagaaaggca gccaaaagaa tgtacgatct
     2161 gacgggtaag catgtctgct atgtggctga ctgcggtggc attgcactgg agaccagcaa
     2221 ggttctcatg accaagaata tagcggtgag tggtagagag gtggtgtaga gagtggagag
     2281 tgttccgaag tggttctggt tcttaaacaa gtctctagtc tctagtctct tggctgctgc
     2341 tggtagctgt cgaaactaat taagttaatg actttttcat gttttgtttt tgtccgctga
     2401 cttttgctgt tgaaagctac aattaagttt atgttttgag tttgatctac ttttgcttaa
     2461 gggaagtact ttcgattacg gattgctggc atataaaaat agataccaag gaatgtgcat
     2521 catatcatat tgattccatc ttgattcttt gcccagaaac tggccatcct tcagagcgtg
     2581 gaaaaccccc cggccatcgc tcagttacaa tcgattaagc acagcacaca gatcttcttc
     2641 tggaccttcg atgtgaccat ggcccgcgag gagatgaaga agtacttcga tgaggtcatg
     2701 gtccagatgg actacatcga tgtgctgatc aatggggcca ccctgtgcga tgagcggaac
     2761 attgatgcca ccatcaatac gaatctgacc ggcatgatga acaccgtggc cactgtgctg
     2821 ccctatatgg accgtaagat gggcggttcg ggtggactga tcgtgaatgt cacctccgtc
     2881 ataggactgg atccatcgcc ggtcttttgt gcgtacagtg cctcaaagtt tggtgtgatt
     2941 gggttcaccc gaagtctagc ggtgagtggg cgagatttta acttgaaaat gctctctaat
     3001 gtcttcttcg gcaggatccc ctgtattaca cccaaaatgg tgtggctgtc atggccgtct
     3061 gctgtggtcc caccaaagtg tttgtcgatc gcgaactgac tgccttcctg gcatacggcc
     3121 aaacctttgc cgatcgcctg cgtcgtgccc cctgccaatc gacggccgtc tgcggcaaga
     3181 atatagtaaa tgccatcgag agatcggaga acggacagat ctggattgcc gacaagggcg
     3241 gcctggagtc ggtggccctc cactggtatt ggcatatggc cgatcagttt gtgaactata
     3301 tgcagagcac tgacgatgag gatcaggagt tttttttggg gcagcgatga ggcatgcctt
     3361 gatccacgga ataaaacatc aaatgtgtca caaaaagtag gcaacttgtt tgtgggtttg
     3421 gaaatagatt tgaaatattt tgaggtcata tgaataatga cataaatgag ggtgcctgaa
     3481 agtatgccaa gttt
//