From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Aspergillus oryzae amdS gene encoding acetamidase.
Date: 21 Jul 92 10:03:30 GMT
Distribution: bionet
Checksum: 46032     7

LOCUS       ASNAMDS      2662 bp ds-DNA             PLN       21-JUL-1992
DEFINITION  Aspergillus oryzae amdS gene encoding acetamidase.
ACCESSION   D10492 D90466
KEYWORDS    acetamidase.
SOURCE      Aspergillus oryzae (strain RIB 40) DNA.
  ORGANISM  Aspergillus oryzae
            Eukaryota; Fungi; Eumycota; Ascomycotina; Plectomycetes;
            Eurotiales; Trichocomaceae.
REFERENCE   1  (bases 1 to 2662)
  AUTHORS   Gomi,K., Kitamoto,K. and Kumagai,C.
  TITLE     Cloning and molecular characterization of the acetamidase-encoding
            gene (amdS) from Aspergillus oryzae
  JOURNAL   Unpublished (1992)
  STANDARD  full staff_review
COMMENT     Data kindly submitted in computer readable form by:
            Katsuya Gomi
            National Institute of Brewing
            2-6-30 Takinogawa
            Kita-ku, Tokyo 114
            Japan
            Phone:  03-3910-6235
            Fax:    03-3910-6239
FEATURES             Location/Qualifiers
     prim_transcript <361..>2302
                     /note="amdS mRNA and introns"
     exon            <361..627
                     /number=1
     CDS             join(361..627,679..819,872..1142,1195..1344,1393..1550,
                     1604..1753,1802..2302)
                     /EC_number="3.5.1.4"
                     /product="acetamidase"
                     /gene="amdS"
                     /codon_start=1
                     /translation="MPSASWEDLAADKRARLEKSIPDEWKFKSVPIEGSVIDLPEKSG
                     ILSPSEIKITNSSATELVAQLANGTLKSVDVTLAFCKRAALAHQLVNCAHDFFPELAL
                     AQARELDRYFETHKKPVGPLHGLPISLKDQLRVKGTETCMAYISWLGKRDTSDSILTA
                     LLRKAGAVFLVKTSVPQTLMVCETVNNIIGRTSNPRNLNLSCGGSSGGEGAMIAMRGG
                     AIGIGTDIGGSIRVPAAFNSLYGIRPSHDRLPYGGMTNSMEGQETIHSVVGPIAHSAQ
                     DVRLFLQSVLKEEPWKYDSKVIPLPWREAEENAAQAKIAEKSLNFAFYDFDGVVRPHP
                     PITRGVEIVRSTLEKDGHTVAPWTPYKHAFAVDLANKIYAADGSTDVYKHINASGEPA
                     IPNIKDLMNPNLPKADLNEVWDAQLQKWRYQCEYLDKWREWEERTGKELDAIIAPVAA
                     TAAVRHNQFRYYGYATVFNVLDYTSVVVPVTYADKAVDHRLADYQPVSDMDKAVYADY
                     DPEVYHGAPVAVQIIGRRLSEERTLAIAEYVGKLLGH"
     CAAT_signal     148..153
     TATA_signal     267..272
     intron          628..678
                     /number=1
     exon            679..819
                     /number=2
     intron          820..871
                     /number=2
     exon            872..1142
                     /number=3
     intron          1143..1194
                     /number=3
     exon            1195..1344
                     /number=4
     intron          1345..1392
                     /number=4
     exon            1393..1550
                     /number=5
     intron          1551..1603
                     /number=5
     exon            1604..1753
                     /number=6
     intron          1754..1801
                     /number=6
     exon            1802..>2302
                     /number=7
BASE COUNT      699 a    631 c    650 g    682 t
ORIGIN      283 bp upstream of StyI site.
        1 aatcagtctg tagaatgctg gaccagcccg aaagcactcc caggcttggt gaatttataa
       61 tggtaaagca atggttatct catgtgggtc cagacaactc ccagaaccac atggccaaga
      121 taaacacgag ataaccggtt ttatcgccca attaaaagct aaaaagcctc acttatgctt
      181 ccatttctcc ttgcgcggtc cgtatggccg cgatgcaaag ttgaggttgc ggggtaaccc
      241 caaatttgag acttggagat tggagatata aagcaacgta ctccatggtc gaatgttctg
      301 tggaaacacc agggtgattg ctctaccatc gtctacagct gtatttatca ctttgtcact
      361 atgccatctg ccagctggga agatctcgct gccgacaaga gggcacgttt ggagaagtcc
      421 atccccgacg aatggaaatt caagtcagtc ccaatagaag gctcggtcat cgatctacct
      481 gagaagtctg ggattctgtc gccttctgaa ataaagatta caaactcgtc tgccacagaa
      541 cttgtcgctc aattagccaa tggcacgttg aagtccgtgg atgtgacact cgcattctgt
      601 aaaagagctg cactggctca tcaacttgtg ggtataacct tcgcctcgat cggagataca
      661 tgaaactaat gagaataggt taattgcgca catgacttct tcccagagct agcactagcc
      721 caggccaggg aacttgatcg gtatttcgag acgcacaaga aacccgtggg accattgcat
      781 ggattaccga tttctttgaa agaccaatta cgagtcaagg taagacgagc ttcctacact
      841 actgtgtgca tctcttctaa catagaacta gggaactgaa acatgcatgg cctatatctc
      901 ttggctgggt aagcgcgaca ccagcgattc gatattgact gccctcttga gaaaagcggg
      961 cgcagtattc cttgttaaga cgagtgtacc acaaacactg atggtatgtg agaccgtcaa
     1021 taatattatc ggtcggacat cgaacccaag gaatctcaac ctttcttgcg gtggtagttc
     1081 gggaggcgaa ggtgccatga ttgcaatgcg tggaggcgcc atcggtatag gaactgatat
     1141 cggtagctat ccatacttgg ttcatcagtt attctggcga ctaatgatat ccaggtggat
     1201 ctattcgtgt cccagccgca ttcaactcct tgtatgggat tcgtccaagt cacgatcgtc
     1261 tgccttacgg tggtatgacg aacagcatgg aaggtcagga aacgatacac agcgtcgttg
     1321 gaccaattgc gcattctgct caaggtaggg atctgggata tttcttcgcg tcgagatact
     1381 gatgctttct agatgtcaga ctcttccttc agtctgtcct taaggaggaa ccttggaagt
     1441 atgattcgaa agtcataccg cttccttgga gggaggccga ggagaacgcc gcccaagcaa
     1501 aaattgctga gaagagtcta aatttcgcat tttacgattt tgatggcgtt gtaagtatta
     1561 gtcgctcctc ctccttcgca atcatgcctg acagttggat aaggtacgtc ctcaccctcc
     1621 gattactcgt ggcgttgaga tcgtccggtc tacgctcgag aaggacggac ataccgtggc
     1681 accctggaca ccctacaagc atgcatttgc cgtagattta gccaacaaaa tctacgctgc
     1741 agatggaagc acggtaagta gcccccctaa gaaaattagt atacgtgcta acatattgta
     1801 ggatgtttac aagcacatca acgcctcagg agaacccgct attccgaaca tcaaggacct
     1861 catgaatccc aacctaccca aggcagattt gaatgaggta tgggacgcgc agctgcaaaa
     1921 atggcgttat cagtgtgaat accttgacaa gtggcgcgaa tgggaggaac ggacgggcaa
     1981 ggagcttgac gctatcatcg ccccggtggc ggcgacagct gcagtccgcc acaaccaatt
     2041 ccggtactat gggtatgcta ctgtctttaa cgtgttagat tacaccagtg ttgttgtccc
     2101 ggttacctat gcagacaagg cggtggatca cagattggcg gattatcagc cggttagtga
     2161 tatggataag gcggtttatg cggactatga tcccgaggtt tatcatggcg cacccgttgc
     2221 cgtgcagatt atcggcagac gtcttagtga ggagcggacc ctggctattg cggagtatgt
     2281 tgggaagttg ttaggtcact agctttcaag ctcagtttta gccactactt gaagcgtctt
     2341 tgtcgagtga gactactgta attagcatac catttacgag gccacgccga gtatttcgcc
     2401 attcaaagca gcttatacgt acctatgggt tgttctgata tatgggtagc ttataatctc
     2461 cactcctcgt attccgaaga tacccaaaaa tcctttacaa tctgtctaat gccttgctga
     2521 atggcgccac atccacaagg tcggtgtcat ctttcgagcc gtaatgtttc taccaaggat
     2581 gtactaatga cactccttga cagcttagac ctactacgca cgcctcgtta tgtgtgtcag
     2641 agacaaataa gcactatcta ga
//