From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: GROESL; Chromatium vinosum; groEL; 2253 base-pairs.
Date: 20 Aug 92 10:01:23 GMT
Distribution: bionet
Checksum: 54482     6

LOCUS       CVNGROESLA   2253 bp ds-DNA             BCT       20-AUG-1992
DEFINITION  GROESL; Chromatium vinosum; groEL; 2253 base-pairs.
ACCESSION   M99443
KEYWORDS    chaperonin protein; groESL gene; inverted repeat.
SOURCE      Chromatium vinosum (strain D) DNA.
  ORGANISM  Chromatium vinosum
            Prokaryotae; Gracilicutes; Anoxyphotobacteria; Purple bacteria;
            Chromatiaceae.
REFERENCE   1  (bases 1 to 2253)
  AUTHORS   Ferreyra,R., Soncini,F. and Viale,A.M.
  TITLE     Cloning, characterization, and functional expression in Escherichia
            coli of chaperonin (groESL) genes from the sulfur phototrophic
            bacterium Chromatium vinosum
  JOURNAL   Unpublished (1992)
  STANDARD  full staff_entry
FEATURES             Location/Qualifiers
     repeat_region   26..52
                     /function="unknown"
                     /rpt_type=inverted
                     /rpt_unit=26..34
                     /rpt_unit=44..52
                     /note="putative"
     RBS             112..117
                     /gene="groES"
                     /note="putative"
     CDS             124..414
                     /function="Chaperonin"
                     /standard_name="GroES"
                     /gene="groES"
                     /evidence=EXPERIMENTAL
                     /codon_start=1
                     /translation="MNIRPLHDRVVVRRMEEERLSAGGIVIPDSATEKPIQGEIIAVG
                     HGKILDNGSVRALDVKVGDSVLFGKYSGTEVKLDGKEFLVMREEDIMAVVEG"
     RBS             475..480
                     /gene="groEL"
                     /note="putative"
     CDS             490..2130
                     /function="Chaperonin"
                     /standard_name="GroEL"
                     /gene="groEL"
                     /evidence=EXPERIMENTAL
                     /codon_start=1
                     /translation="MSAKDVKFGGDARVRMMEGVNILANAVKVTLGPKGRNVVLEKSF
                     GAPTVTKDGVSVAKEIELKDKFENMGAQMVKEVASKTSDIAGDGTTTATVLAQAMVRE
                     GLKAVAAGMNPMDLKRGMDKAVEAATEELKKLSKPCPRPMAIAQVGTISANSDDSIGT
                     IIAEAMEKVGKEGVITVEDGTSLQNELDVVEGMQFDRGYLSPYFINNQQSQSAELDAP
                     YILLYDKKISNIRDLLPVLEGVAKAGKPLLIIAEDVEGEALATLVVNTIRGIVKVCAV
                     KAPGFGDRRKAMLQDIAILTGATVISEEVGLSLEKATLTDLGTAKRVQVGKDETTIID
                     GSGSEIDIKARCEQIRAQVEETSSDYDREKLQERLAKLAGGVAVIKVGAATEIEMKEK
                     KARVEDALHATRAAVEEGIVPGGGVALVRAIAAVKDLKGANHDQDVGIAIARRAMEEP
                     LRQIVANAGEEPSVILHKVAEGTGNFGYNAANGEYGDMVEMGILDPTKVTRSALQNSC
                     SVAGLMITTEAMIADEPKDDAPAMPGGGMGDMGGMGMM"
     repeat_region   2142..2161
                     /rpt_type=inverted
                     /rpt_unit=2142..2149
                     /rpt_unit=2154..2161
                     /note="putative"
     repeat_region   2180..2193
                     /rpt_type=inverted
                     /rpt_unit=2180..2184
                     /rpt_unit=2189..2193
                     /note="putative"
BASE COUNT      465 a    709 c    714 g    365 t
ORIGIN
        1 gacagccgac gatttcgctc taccattagc actcgttaca agtgagtgct aacaggccgg
       61 cccggtgtcg gtctcgccgc gactcgaccc acccacttct caacctgtat cgaggagcca
      121 tccatgaaca tccgtcccct gcatgaccgc gtcgtcgtcc gtcgcatgga agaggagcgc
      181 ctgagcgccg gcggcatcgt gatcccggat tccgccaccg agaagccgat ccagggcgag
      241 atcatcgccg tcggccacgg caagatcctc gacaacggca gcgtgcgcgc actcgacgtc
      301 aaggtcggcg acagcgtgct gttcggcaag tattccggca ccgaggtcaa gctcgacggc
      361 aaggaattcc tggtgatgcg cgaagaagac atcatggcgg tcgtcgaggg ctgatcggcc
      421 ttccgcccct gagtctccaa gcttttccca atcaagacag aaaacgattc attcgaggaa
      481 tttagaagca tgagcgcaaa agacgtgaag ttcggcggtg atgcccgtgt ccgcatgatg
      541 gagggtgtca acatcctcgc caacgccgtc aaggtcacgc tgggtccgaa gggccgcaac
      601 gtggtgctgg agaagtcctt cggtgccccg accgtcacca aggacggcgt ctccgtggcc
      661 aaggagatcg agctcaaaga caagttcgag aacatgggcg cgcagatggt caaggaagtc
      721 gcttccaaga cctccgacat cgccggtgac ggcaccacca ccgcgaccgt gctggctcag
      781 gccatggtcc gtgagggtct gaaggcggtc gccgccggca tgaacccgat ggatctgaag
      841 cgcggcatgg acaaggccgt cgaggccgcc accgaagagc tcaagaagct ctccaagccc
      901 tgtccgagac caatggcgat agctcaggtc ggaaccatct cggccaactc cgacgactcg
      961 atcggcacca tcatcgccga ggcgatggag aaggtcggca aggaaggcgt catcaccgtc
     1021 gaagacggaa cctcgctgca gaacgagctg gacgtggtcg agggcatgca gttcgatcgc
     1081 ggctacctgt cgccctactt catcaacaac cagcagagcc agagcgccga gctcgacgcg
     1141 ccctacatcc tgctgtacga caagaagatc tccaacatcc gtgatcttct ccccgtgctg
     1201 gaaggtgtcg ccaaggccgg caagccgctg ctgatcatcg ccgaagacgt cgagggcgaa
     1261 gccctggcga ccctggtcgt caacaccatc cgcggcatcg tcaaggtctg cgccgtcaag
     1321 gcgccgggct tcggtgatcg tcgcaaggcc atgcttcagg acatcgcgat cctgaccggt
     1381 gcgaccgtga tttcggaaga agtcggcctg tcgctggaga aggcgaccct gaccgatctg
     1441 ggtaccgcca agcgcgtcca ggtcggcaag gacgagacca ccatcatcga cggctccggt
     1501 tccgagatcg acatcaaggc ccgttgcgag cagatccgcg ctcaggtcga agagaccagc
     1561 tccgactacg atcgtgagaa gctccaggag cgtctggcca agctggccgg tggtgtggcc
     1621 gtgatcaagg tcggcgccgc caccgagatc gagatgaagg agaagaaggc gcgcgtcgaa
     1681 gacgctctgc acgccacccg tgccgccgtc gaggaaggta tcgtccccgg tggtggtgtc
     1741 gcgctggttc gcgccatcgc agccgtcaag gatctcaagg gcgccaacca cgatcaggac
     1801 gtcggtatcg ccatcgcccg tcgcgcgatg gaagagccgc tgcgtcagat cgtcgccaac
     1861 gccggtgaag agccgtcggt catcctgcac aaggttgctg agggcacggg taacttcggt
     1921 tacaacgccg ccaacggcga gtacggcgac atggtcgaga tgggcatcct ggacccgacc
     1981 aaggtcaccc gttcggctct gcagaactcc tgctcggtcg ccggtctgat gatcaccacc
     2041 gaagccatga tcgccgacga gccgaaggac gacgctccgg cgatgccggg cggcggcatg
     2101 ggcgacatgg gcggcatggg catgatgtaa gcccgcgcgc tccgggacgt tcgcgtcccg
     2161 gctcgacgcc agaaaaagcc ccgcttcggc gggcgttttt cgtgggcgct ttcagtccag
     2221 gctcgtcgtg gacggttcga ggatggcgcc gat
//