From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Nematode choline acetyltransferase (cha-1) gene exons 1-11,
Date: 26 Jan 93 05:46:40 GMT
Distribution: bionet
Checksum: 30730 13

LOCUS       CELCHA1Y     5212 bp ds-DNA             INV       25-JAN-1993
DEFINITION  Nematode choline acetyltransferase (cha-1) gene exons 1-11,
            complete cds.
ACCESSION   L08970
KEYWORDS    choline acetyltransferase.
SOURCE      Caenorhabditis elegans (strain Bristol N2) DNA.
  ORGANISM  Caenorhabditis elegans
            Eukaryota; Animalia; Eumetazoa; Nematoda; Secernentea; Rhabditida;
            Rhabditina; Rhabditoidea; Rhabditidae.
REFERENCE   1  (bases 1 to 5212)
  AUTHORS   Alfonso,A., Grundahl,K., McManus,J.R. and Rand,J.B.
  TITLE     Cloning and characterization of the choline acetyltransferase
            structural gene (cha-1) from C. elegans
  JOURNAL   Unpublished (1993)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     intron          <1..469
                     /number=1
                     /evidence="EXPERIMENTAL"
                     /citation=[1]
     exon            470..520
                     /number=2
                     /gene="cha-1"
                     /evidence="EXPERIMENTAL"
                     /citation=[1]
     intron          521..1003
                     /number=2
                     /gene="cha-1"
                     /evidence="EXPERIMENTAL"
                     /citation=[1]
     exon            1004..1053
                     /number=3
                     /gene="cha-1"
                     /evidence="EXPERIMENTAL"
                     /citation=[1]
     intron          1054..1584
                     /number=3
                     /gene="cha-1"
                     /evidence="EXPERIMENTAL"
                     /citation=[1]
     exon            1585..1726
                     /number=4
                     /gene="cha-1"
                     /evidence="EXPERIMENTAL"
                     /citation=[1]
     intron          1727..1769
                     /number=4
                     /gene="cha-1"
                     /evidence="EXPERIMENTAL"
                     /citation=[1]
     exon            1770..1942
                     /number=5
                     /gene="cha-1"
                     /evidence="EXPERIMENTAL"
                     /citation=[1]
     intron          1943..2013
                     /number=5
                     /gene="cha-1"
                     /evidence="EXPERIMENTAL"
                     /citation=[1]
     exon            2014..2290
                     /number=6
                     /gene="cha-1"
                     /evidence="EXPERIMENTAL"
                     /citation=[1]
     intron          2291..2334
                     /number=6
                     /gene="cha-1"
                     /evidence="EXPERIMENTAL"
                     /citation=[1]
     exon            2335..2503
                     /number=7
                     /gene="cha-1"
                     /evidence="EXPERIMENTAL"
                     /citation=[1]
     intron          2504..2546
                     /number=7
                     /gene="cha-1"
                     /evidence="EXPERIMENTAL"
                     /citation=[1]
     exon            2547..2788
                     /number=8
                     /gene="cha-1"
                     /evidence="EXPERIMENTAL"
                     /citation=[1]
     intron          2789..3052
                     /number=8
                     /gene="cha-1"
                     /evidence="EXPERIMENTAL"
                     /citation=[1]
     exon            3053..3276
                     /number=9
                     /gene="cha-1"
                     /evidence="EXPERIMENTAL"
                     /citation=[1]
     intron          3277..3393
                     /number=9
                     /gene="cha-1"
                     /evidence="EXPERIMENTAL"
                     /citation=[1]
     exon            3394..3592
                     /number=10
                     /gene="cha-1"
                     /evidence="EXPERIMENTAL"
                     /citation=[1]
     intron          3593..3835
                     /number=10
                     /gene="cha-1"
                     /evidence="EXPERIMENTAL"
                     /citation=[1]
     exon            3836..4315
                     /number=11
                     /gene="cha-1"
                     /evidence="EXPERIMENTAL"
                     /citation=[1]
     polyA_signal    4295..4300
                     /gene="cha-1"
     polyA_site      4313
                     /gene="cha-1"
                     /evidence="EXPERIMENTAL"
                     /citation=[1]
                     /note="cDNA RM#17L poladenylated following residue #4313;
                     cDNARM#69P polyadenylated following residue #4315"
     polyA_site      4315
                     /gene="cha-1"
     CDS             join(470..520,1004..1053,1585..1726,1770..1942,2014..2290,
                     2335..2503,2547..2788,3053..3276,3394..3592,3836..4192)
                     /function="synthesis of acetylcholine"
                     /EC_number="2.3.1.6"
                     /standard_name="ChAT"
                     /gene="cha-1"
                     /citation=[1]
                     /note="putative"
                     /product="choline acetyltransferase"
                     /codon_start=1
                     /translation="MEKEKVDELPPNDNWYETALPKPPVPSLEATLDRYLEYAAVVAV
                     GQKASLATTHDAAHKFVRQATPLQEQLLEIAEKSPNWATKFWLPEMYMRVRMPTPVNS
                     NPGYIFPKVKFETKEDHIKYTALLTRGLLEYKNLIDTKQVCREKSTGAQKLQMCMEQY
                     DRVLSCYREPGVGEDTQIRKQKTNDGNEHVLVMCRNQTFLLHSRINGALVSYADVEYQ
                     LAQIEEISKINQNNTANIGASGVGPRDNAALFWQDMLTVEQNSKSYEWVKSALFVVCL
                     DMEDPIDYGKNDTMSISEKEKEFVARGYSTLTGHGSSKFGLNRWYDATIQLVVSSSGV
                     NGLCIEHSTAEGIVIINMAETAIRYAQKYFKSKMVWNDVRNVHPKSLTWHFSENSRNI
                     LKKQAEVFDELANELELEVLIFNEFGKDSIKNWRVSPDGFIQLIMQLAHYKTHGHLVS
                     TYESASVRRFGAGRVDNIRANTQEALEWVTAMASKKESKERKLELFKKAVLKQVKVTL
                     ENISGYGVDNHLCALFCLAREREETTGEDIPSLFLDPLWSEVMRFPLSTSQVTTSLDI
                     PDCYLTYGAVVRDGYGCPYNIQPDRVIFAPTAFRSDPRTDLQHFKKSLAGAMRDVKEL
                     LSN"
BASE COUNT     1522 a   1058 c   1075 g   1557 t
ORIGIN      
        1 gtcgacttat atatatattc ttgacacttg gttagtgttt tagcttaaaa ttaaaaaatc
       61 tcaatctaaa gattgtttca gcaaaaaaag tgtcttagtc ttttgaaggg atattgattt
      121 ggagttgttt aaaagacagt gcaatgtaaa attaatgtag tttttcggaa aattaatatt
      181 aattttggtg ggatattgag ctagtattag tgggggatcg gtgtcgggct actgtagtga
      241 tttcgtatag agtaatgtca aattttggca gaaactaaga tttctgtagc ctaggcccaa
      301 gcctaagcct aaacctaagc ctcatactac gcctaagcct acgcctaagc aaaagcatga
      361 acataagcct aagcgtaagc ctaagcctaa gcctgagtct aagcctaatt tgcaaaatat
      421 acacaatttt gaaagtataa cagatactaa gaagtaaatt ttgttccaga tggaaaagga
      481 aaaagttgac gaacttccac caaatgacaa ttggtatgag gtaagtaaaa ccaccgctca
      541 tgaacctttt ttgtactcct acactcccat ggggcaagaa acctttcgat ttaattagat
      601 ttgaaaattg tgagagattt cacgcaaaaa atcgtatcta gtgcatcttt cccctgctat
      661 ttctttttgt tcgaacaaaa aagcaccaaa aacccattaa cagctagctg gctcatttcg
      721 aagaggcttc tgtctttctt catcccctta gcaagaagaa ctcggtcccc tttttcaact
      781 gagaactaat tagtttgaaa caatttttct tgtttcgcct tctcgatcac caccaagaca
      841 ctccctcttt ttttgaaaat aaatttcaaa aaagtattat gagaattgtg gcatttttcc
      901 taaaaaatca attcttcccc atacaaattg cttaccgtaa tcttaagagt gtaaaggtaa
      961 agttggctga aatattaaat tttaagcttt aaaaaatttt cagactgcac tgccaaagcc
     1021 gccggtacca agcctggagg ccacgctaga taggtaagtg tctttcaaaa ttgatcagca
     1081 ggtggccata aattacctct cacagaaata ttttgcaaat ggaatgtcaa ttgatagcac
     1141 ttaagtttac aatttagcat caaacaacaa attttgacgc caataatagc tgattttatc
     1201 tcggttggct ttggtttaat ctaaaaactt tcaactaatc taagttttaa aataaatgat
     1261 tctatagttt tgtagtttga aatttattaa tacttttaaa attagccgag atataacctg
     1321 ccaaagttaa agtgcctgga tatggtgcat cgataaacta gctatgacaa atttcagagg
     1381 ctcatatccg ggctgtcatt ggttttatca aacaaagtat aactaaataa tttttgaaat
     1441 atgtttctct atagtattgt aggttaaaat gtatcaatat cttaaaaatg aactgagata
     1501 tgatcatttg aatttgcgtc cgacatctca cgagcccgtt aagaaactcg cgaggtgtcc
     1561 gccgcttctc aaactaaatt ccagatatct cgaatacgct gccgttgtag ccgtaggcca
     1621 gaaagcctca ctagcgacaa cccacgatgc agctcacaag ttcgtgcgtc aagctactcc
     1681 gttacaggag caactgctgg aaattgcgga gaagtcgccg aattgggtgg gttctcgcaa
     1741 gttttctaat tgaaaaaaaa acatttcagg ccacaaaatt ttggctcccc gagatgtata
     1801 tgagagttag gatgccaacg cccgtgaatt caaatccggg gtatattttt ccgaaagtca
     1861 aatttgagac aaaggaggat cacatcaaat atacggcatt gctgacaaga gggctcttgg
     1921 agtacaagaa cttgatagac acgtgagtac agtaccccta cagtatccct atagtctggc
     1981 aagacaccta ccaccgccgt cgatattttc cagaaaacaa gtgtgccgag aaaagtccac
     2041 tggcgcgcaa aaactccaga tgtgcatgga acagtacgac cgagttctca gctgctacag
     2101 ggagccgggc gtcggcgagg atacccaaat acgaaagcag aagacgaatg atgggaatga
     2161 acatgtactc gtgatgtgcc ggaatcagac ttttttgctc cattcgcgaa tcaatggagc
     2221 tctagtgtca tatgcagatg tggagtacca gctggcgcag atcgaggaga tttctaagat
     2281 taatcaaaac gtgggaccct gagttcaaaa tgaaaagttt aagaatgact tcagaacacc
     2341 gcaaatatcg gagcctctgg cgttggcccg cgtgataacg cggctctttt ttggcaggat
     2401 atgctcacag tcgagcagaa ctcaaaatcc tatgaatggg taaagtcggc gcttttcgtg
     2461 gtgtgtcttg acatggaaga tcctattgat tatgggaaaa atggtctgag aacttgcatg
     2521 tacctccaac cttgaaaaag tttcagatac catgagcatt tccgagaagg agaaggaatt
     2581 tgtggcgcgt ggctactcaa ctctgaccgg ccatggaagt tcaaagttcg ggctcaatcg
     2641 gtggtatgat gcgactattc agctggtggt gtcttccagt ggtgtgaatg ggttgtgcat
     2701 agagcactcg acggccgagg ggattgtgat tattaatatg gctgaaactg ctatcagata
     2761 tgcacagaag tactttaaaa gcaagatggt gaggtttttt gcgttgggct gtaggactgt
     2821 agactgtagg agtgatgtag gggtactgta gatatacggt atggtgttac tgtaaaagta
     2881 ctgttgaggt actgtaggga gcttttgact agtacacagg ctcggtaatg ttgattaaaa
     2941 tactgtagtg gagtgatttg tacatgtgta attcacaaac tgtagctgta ctttaaatgt
     3001 aatggatact gtagaatcgc attattaact ataaatttgc atattttttt aggtttggaa
     3061 cgacgtgcgc aatgtacacc ccaaatctct gacatggcat tttagtgaaa attctagaaa
     3121 tattctgaaa aagcaggcgg aagttttcga tgagctcgcc aacgaactgg agcttgaagt
     3181 tttgattttc aacgaattcg gcaaagattc catcaaaaat tggcgtgtca gtccggatgg
     3241 atttattcag ttgattatgc aattggcgca ttataagttg gttttttagt caaactatat
     3301 cataatttct gcaaatagtc cctggtgtta aggaatcact gattagagta gaaacaggac
     3361 acacacacaa atatttactc gctatttttt cagaacacat ggtcacctcg tttccaccta
     3421 cgaatcagct tcggttcggc gattcggtgc gggaagagtt gataacattc gggcgaacac
     3481 acaagaggcg ctggaatggg ttacggcaat ggcgagcaag aaggagtcta aagagagaaa
     3541 gctggaatta tttaagaagg ctgtgctcaa gcaggtcaag gtcacgttgg aggtaagaaa
     3601 aacggaacac ttagcctaag cctaagccta atcctaagca taagcctagc attaagtaag
     3661 gcaatagctc ttcccatatt ttggctctgg ccctgcccat accttgttct ttcttggctc
     3721 tatctccgct tatatcttgg ccccgcctat gtcttgactc tagctccgct caaatatata
     3781 tcggctcaga ctccgcctat gtcttggcat cgccaataaa tttaaagttt ttcagaacat
     3841 cagcggctac ggtgtcgaca atcatctttg cgccctattc tgtctcgccc gggaaaggga
     3901 agaaacgacc ggagaagata taccatcact gtttctggat cctctgtggt ctgaagttat
     3961 gcgattccca ttgtcgacaa gccaagtcac aacctctctg gatatccctg attgctacct
     4021 gacgtatggt gcagtggtcc gcgatggcta tggatgcccg tacaatattc aaccggatcg
     4081 agtgattttt gcgccgactg cgttcagaag tgatccgagg actgatttac aacattttaa
     4141 gaaaagcttg gctggagcta tgagagatgt caaggagctg ctgagcaact gaattttatt
     4201 attatttttt gagatatttt ttatttaatt tggcaataat ttgaatatag agatgcatgt
     4261 agttgagtgc tcagaatttt ttcccttaat tggaaataaa gtggatttgt atatactttt
     4321 ctgtctccta aatatttttt ttgtcaactt tcgccaaact ttttctttct ttttcaaaac
     4381 catctcatac ttttggcaat tttcgttaca ctgtcgcaaa tgattgtact tgacaattta
     4441 tattataaaa tttgacaact gcatcaacaa acttttctca gcttttgcca aataatcttc
     4501 gagcggctct aaattttttg ataaattctt tcgccaattc ttgcaactct ctcttcaact
     4561 tttgtcaatc tttgccaatt ttcctcaaat gttctcttaa accttgtaca ttccaaccaa
     4621 aactagatcg tcatcacaat cttcaaaaaa cccgggaaca aagtctgagg ttccattccc
     4681 tccacgtcac gatctattgg cctctccact acctgccacg tcatcccaga aacaccagac
     4741 tccgccccaa actgtctcca ggcgaattgt gcaacgtgat gctttttttg ctcgacacgt
     4801 tacgtcagcc gcccgcctga aacgtcgttt tcagcacctt caatcagctt atcgcggagt
     4861 ttcaggcaca cactttgtgc tgataaaaaa catgattgag caaaaacgga atttcatttt
     4921 tgttccccca ccggcgccgg ctcggggctc tcacttttga tttatgtccc cattcatgtc
     4981 cctgcaggag ggcttaccaa atttcgagaa tttttcgaga agcgataagc ttgtatgagt
     5041 gaccggtacc tggtacttat gaccgccgcc gccgccctct ccgttcatat tttgtcacat
     5101 gtgactgtgt gcacattttt ggctcttttt cgagtttttt ttaacaattg tttattttaa
     5161 aaaaccgttt cagaatgtgc caattacata atttccgtcc aaaacgggat cc
//