From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Homo sapiens (clone JH4B1) PM-scl autoantigen mRNA, complete cds.
Date: 11 Sep 92 10:04:13 GMT
Distribution: bionet
Checksum: 30335     7

LOCUS       HUMAUA       2739 bp ss-mRNA            PRI       11-SEP-1992
DEFINITION  Homo sapiens (clone JH4B1) PM-scl autoantigen mRNA, complete cds.
ACCESSION   L01457
KEYWORDS    PM-scl autoantigen.
SOURCE      Homo sapiens (library: lambda-gt11) thymus cDNA to mRNA.
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 2739)
  AUTHORS   Ge,Q., Frank,M.B., O'Brien,C.A. and Targoff,I.N.
  TITLE     Cloning of a complementary DNA coding for the 100-kD antigenic
            protein of the PM-Scl autoantigen
  JOURNAL   J. Clin. Invest. 90, 559-570 (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     5'UTR           1..38
     CDS             39..2621
                     /standard_name="100 kD Pm-Scl antigenic protein"
                     /note="The deduced amino acid sequence contains 860
                     residues with apredicted molecular mass of 98,088 daltons.
                     Western blotsindicate a 100 kD protein."
                     /product="PM-Scl autoantigen"
                     /evidence=EXPERIMENTAL
                     /codon_start=1
                     /translation="MAPPSTREPRVLSATSATKSDGEMVLPGFPDADSFVKFALGSVV
                     AVTKASGGLPQFGDEYDFYRSFPGFQAFCETQGDRLLQCMSRVMQYHGCRSNIKDRSK
                     VTELEDKFDLLVDANDVILERVGILLDEASGVNKNQQPVLPAGLQVPKTVVSSWNRKA
                     AEYGKKAKSETFRLLHAKNIIRPQLKFREKIDNSNTPFLPKIFIKPNAQKPLPQALSK
                     ERRERPQDRPEDLDVPPALADFIHQQRTQQVEQDMFAHPYQYELNHFTPADAVLQKPQ
                     PQLYRPIEETPCHFISSLDELVELNEKLLNCQEFAVDLEHHSYRSFLGLTCLMQISTR
                     TEDFIIDTLELRSDMYILNESLTDPAIVKVFHGADSDIEWLQKDFGLYVVNMFDTHQA
                     ARLLNLGRHSLDHLLKLYCNVDSNKQYQLADWRIRPLPEEMLSYARDDTHYLLYIYDK
                     MRLEMWERGNGQPVQLQVVWQRSRDICLKKFIKPIFTDESYLELYRKQKKHLNTQQLT
                     AFQLLFAWRDKTARREDESYGYVLPNHMMLKIAEELPKEPQGIIACCNPVPPLVRQQI
                     NEMHLLIQQAREMPLLKSEVAAGVKKSGPLPSAERLENVLFGPHDCSHAPPDGYPIIP
                     TSGSVPVQKQASLFPDEKEDNLLGTTCLIATAVITLFNEPSAEDSKKGPLTVAQKKAQ
                     NIMESFENPFRMISNRWKLAQVQVQKDSKEAVKKKAAEQTAAREQAKEACKAAAEQAI
                     SVRQQVVLENAAKKRERATSDPRTTEQKQEKKRLKISKKPKDPEPPEKEFTPYDYSQS
                     DFKAFAGNSKSKVSSQFDPNKQTPSGKKCIAAKKIKQSVGNKSMSFPTGKSDRGFRYN
                     WPQR"
                     /citation=[1]
     3'UTR           2622..2739
     polyA_signal    2700..2705
                     /note="putative"
     misc_difference replace(2715..2719,"aa")
                     /note="2 of 8 PCR product of the 3' untranslated region
                     showed nucleotides 2716-2718 missing."
                     /citation=[1]
BASE COUNT      830 a    685 c    651 g    573 t
ORIGIN
        1 gacaagctct cgcgagacga gccgtgcagg ctgaaaaaat ggcgccaccc agtacccggg
       61 agcccagggt cctgtcggcg accagcgcaa ccaaatccga cggagagatg gtgctgccag
      121 gcttcccgga cgccgacagc tttgtgaagt ttgctcttgg gtccgtggtg gcagtcacca
      181 aggcatctgg gggcctacca cagtttggcg atgagtatga tttttaccga agttttcctg
      241 gcttccaagc attttgcgaa acacagggag acaggttgct tcagtgcatg agcagagtaa
      301 tgcagtacca tgggtgtcgc agcaacatta aggatcgaag taaagtgact gagctggaag
      361 acaagtttga tttactagtt gatgccaatg atgtaattct ggagagagtg ggtattttac
      421 tggatgaagc ctcaggtgta aacaagaatc aacagcctgt cctccctgcc ggcttgcagg
      481 tccccaaaac ggtagtgtcc agctggaacc gtaaggcagc agaatatggc aaaaaagcaa
      541 aatctgaaac tttccggctg cttcatgcaa aaaatatcat ccgacctcag ctcaagtttc
      601 gagagaagat tgacaattcc aacacaccat ttcttcctaa aatcttcatc aaacccaatg
      661 ctcagaaacc tctccctcaa gctctctcta aggaaaggcg ggaacgccca caggatcgtc
      721 ctgaggactt ggacgtcccc cctgcactgg ctgatttcat ccatcagcag agaacccagc
      781 aggttgagca agacatgttt gcacatcctt atcaatatga actaaatcac tttaccccag
      841 cagatgcagt gcttcaaaag ccacaacccc agttatacag acctatagaa gagacaccat
      901 gccatttcat atcctccctg gatgaactcg tggaactcaa cgaaaagctc ttgaattgtc
      961 aggaatttgc agttgacttg gagcaccact cttacaggag cttcctggga ctgacctgcc
     1021 tgatgcaaat ttctactcgg acggaagact tcatcattga caccctcgag cttcgaagtg
     1081 acatgtacat tctcaatgag agcctcacag acccagccat cgttaaggtc tttcatggtg
     1141 ctgattcaga catagaatgg ctacagaaag actttgggtt gtatgtagta aacatgtttg
     1201 atactcatca ggcagcacgc cttcttaacc tgggcaggca ctcactcgat catctcctga
     1261 aactctactg caacgtggac tcaaacaagc aatatcagct ggctgattgg agaatacgcc
     1321 ctctgcccga ggagatgctc agctacgccc gggatgacac ccattacctg ctatatatct
     1381 atgacaaaat gaggctggag atgtgggagc gcggcaacgg gcagcccgtg cagctgcagg
     1441 tggtgtggca acggagcagg gacatctgcc tcaagaaatt catcaaacct atcttcacgg
     1501 atgagtccta ccttgaactc tataggaagc agaagaagca ccttaacaca cagcagttga
     1561 cagcctttca gctgctgttt gcctggaggg ataaaacagc tcgcagggaa gatgaaagtt
     1621 acggatatgt actgccaaac cacatgatgc tgaaaatagc tgaagaactg cctaaggaac
     1681 ctcagggcat catagcttgc tgcaacccag taccgcccct tgtgcggcag cagatcaacg
     1741 aaatgcacct tttaatccag caggcccgag agatgcccct gctcaagtct gaagttgcag
     1801 ccggagtgaa gaagagcgga ccgctgccca gtgctgagag attggagaat gttctctttg
     1861 gacctcacga ctgctcccat gcccctccgg atggctatcc aatcatccca accagtggat
     1921 ctgtgccagt tcagaagcag gcgagcctct tccctgatga aaaagaagat aacttgctgg
     1981 gtaccacatg cctgattgcc acagctgtca tcacgttatt taatgaacct agtgctgaag
     2041 acagtaaaaa gggtccattg acagttgcac agaaaaaagc ccagaacatc atggagtcct
     2101 ttgaaaatcc atttaggatg atcagcaacc gttggaagct ggcccaggta caagtacaaa
     2161 aagactctaa agaagctgtc aagaagaagg cagctgagca aacagctgcc cgggaacagg
     2221 caaaggaggc gtgcaaagct gcagcagaac aggccatctc cgtccgacag caggtcgtgc
     2281 tagaaaatgc tgcaaagaag agagagcgag caacaagcga cccaaggacc acagaacaga
     2341 aacaagagaa gaaacgactc aaaatttcca agaagccaaa ggacccagag ccaccagaaa
     2401 aagagtttac gccttacgac tacagccagt cagacttcaa ggcttttgct ggaaacagca
     2461 aatccaaagt ttcttctcag tttgatccaa ataaacagac cccgtctggc aagaaatgca
     2521 ttgcagccaa aaaaattaaa cagtcggtgg gaaacaaaag catgtccttt ccaactggaa
     2581 agtcagacag aggcttcagg tacaactggc cacagagata gtcctggaag acacgtggcg
     2641 cctgtggacc ggaagcacca aatgctggtg ctgcttttgt acatacatat ttttaaacca
     2701 ttaaaattct tcctgaagaa aaaaaaaaaa aaaaaaaaa
//