From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Rhizobium fredii nodulation protein (nodU) gene, complete cds.
Date: 29 Dec 92 19:51:19 GMT
Distribution: bionet
Checksum: 15295 6

LOCUS       RHMNODUEX    2703 bp ds-DNA             BCT       09-DEC-1992
DEFINITION  Rhizobium fredii nodulation protein (nodU) gene, complete cds.
ACCESSION   L03520
KEYWORDS    nodU gene; nodulation protein.
SOURCE      Rhizobium fredii (strain USDA 257) DNA.
  ORGANISM  Rhizobium fredii
            Prokaryotae; Gracilicutes; Scotobacteria; Rhizobiaceae.
REFERENCE   1  (bases 1 to 2703)
  AUTHORS   Krishnan,H.B., Lewin,A., Fellay,R., Broughton,W.J. and Pueppke,S.G.
  TITLE     Differential expression of nodS accounts for the varied abilities
            of Rhizobium fredii USDA257 and Rhizobium sp. NGR234 to nodulate
            leucaena spp
  JOURNAL   Mol. Microbiol. 6, 3321-3330 (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     CDS             375..902
                     /codon_start=1
                     /translation="MRSSRSDIRRCFGCRFPKVLSPTHSKSGARPAIHGKLAPYCKRL
                     TVIDVVPRAIARTRQRMKESSHINWIVADVRQFSTQQLFDLIVVAEVLYYLEDVAAIR
                     TAVHNLVSMLAPSGHMVFGSAIDANCRRWGHVAGTETVIAMLNETLIEVEQLYCRGAS
                     VNEDCLLSRFQKSTT"
     CDS             859..2604
                     /function="homologous to the host-specificity gene of
                     Rhizobium NGR234"
                     /gene="nodU"
                     /evidence="EXPERIMENTAL"
                     /product="nodulation protein"
                     /codon_start=1
                     /translation="MKTACFPVFRNRLPDLDRDLDRDTKRVCGIKLTHDGAIAVVEDG
                     RLVFCTEQEKRNNNSRYQEINNLDAVVAALAENGVNARDVDQFVIDGWDGEAESRFKV
                     LSGETPVILRGAPYVERHAEGLLDWIGGSGLTLGDRVFSYRSYPHVTSHVASAYCTSP
                     FAKSGDPALCLVWDGCIFPQLYHVEGKRASFVKSLFPVTGQAYAAAGHYFGPYKQTSR
                     GGWDLGVAGKLMAFVALGSVHVRIVAVFQKLYQEHFAGDTALACAFRANINNSESSLA
                     AVHDFFAASALQLGQRRPKTCLHRLIFSSNVSSLTKWRTLQHHPLPGARNLCIAGGCG
                     LNIKWNSALRQTGLFDSVWVPPFPNDSGSAIGAACCEIVAQQGFVPLDWSVYSGPSLQ
                     DRQVPAGWHASPCSISEVAAILASNKPVVFLSGRTELGPRALGGRSILAAATSPEMKD
                     HLNEIKFREHFRPVAPICLEDRAPDIFSPGTPDPYMLFDHQTKMPWQDKVPAVVHLDG
                     SARLQTISRNSQHKVAEVLVEYEKLTGIPLLCNTSANYHGRGFFPSAAAACEWGRVEH
                     VWCDGMLYRKPSATA"
BASE COUNT      582 a    756 c    763 g    602 t
ORIGIN      
        1 ctctcaacga tcgggcgact actccaactc ttcactccag acgaatgcga tcaagccgag
       61 cagcccccaa cacgcccgcc cttggagtga ccggaactgg cgaaacttcc ataacctgga
      121 tgtttggcat cacaaaaatc gattttactt cgagcgaact caagggccca caagtagcag
      181 tattgacgga gccatcgttg ccggcgaccg ctttctcggg aattcgcgtg cattccacgc
      241 ctccggattc gcctcgctct ccccacccag caaaatcgtg cacagtgttt cggagccaaa
      301 tttgacgcgg gtcaacaact atcatttatt gcaccgtgag ctggcggagg aggacccatg
      361 gcggctcgac gccaatgcgt tcgagcagga gcgacattcg cagatgcttc ggttgtcgtt
      421 ttcccaaggt cctatcacca acgcactcga agtcgggtgc gcggccggcg attcacggaa
      481 aactggcgcc ttattgcaag cggctgactg tgattgatgt ggtgccgcga gcgattgctc
      541 gaacgaggca acggatgaag gaatcgtctc acatcaactg gatagtcgct gacgttcgac
      601 agttctcgac tcagcagcta ttcgatctga tcgtcgtggc cgaagttctt tattatctcg
      661 aggatgtagc cgcgatacgc accgccgttc acaacctggt gagcatgctg gcgccaagtg
      721 ggcacatggt ttttggatcc gcaatcgatg ctaattgccg gcgctggggg catgtggccg
      781 gtaccgagac cgtcatcgcg atgttgaacg aaacgttgat cgaggtggag cagctttatt
      841 gccgcggcgc atcggtcaat gaagactgct tgctttcccg ttttcagaaa tcgactacct
      901 gatttggata gagatttgga tagagacact aagcgcgtct gtggcatcaa gttaacccat
      961 gacggggcta tcgccgttgt cgaggatgga aggctcgtct tctgtactga gcaggagaag
     1021 cggaataaca attcgcgcta tcaagaaatc aacaatctcg atgctgttgt ggcggctctg
     1081 gcggaaaacg gggtcaatgc aagagatgtt gatcagtttg tcatcgacgg ctgggacggt
     1141 gaggccgagt cgcggttcaa ggtcctcagt ggggagactc ctgttatcct cagaggcgcg
     1201 ccttacgttg aacgtcacgc cgagggactt ctcgattgga tcggcggctc cggcctcaca
     1261 cttggtgatc gggtttttag ctacagaagc tatccgcatg tgacgagcca tgtcgcctct
     1321 gcatactgca ccagcccctt tgccaaatcc ggagaccctg cgctttgcct ggtgtgggac
     1381 ggctgcatat ttccgcagct ctaccatgtg gaaggcaagc gagccagctt cgtcaaatcc
     1441 ttgttcccgg taacaggtca ggcctacgct gccgcgggcc actacttcgg cccctataag
     1501 cagacgagcc gcgggggctg ggaccttggc gttgccggca aactgatggc ctttgtcgca
     1561 ctgggatcag ttcacgtacg catcgttgct gtgttccaaa agctctatca agaacacttt
     1621 gccggcgata ctgcgcttgc ctgcgccttc cgtgcgaaca tcaacaactc ggaatcctca
     1681 cttgcggccg tgcacgattt tttcgctgcg agcgcgctcc aattggggca gaggcgcccg
     1741 aagacgtgct tgcatcgtct cattttttcc tcgaacgtct cctcgttgac gaaatggcga
     1801 accttgcagc accatcccct gccgggagca cgcaatctgt gcatagctgg cggctgtgga
     1861 ctcaatatta aatggaacag tgcgctgcgg cagacaggcc tgttcgattc ggtttgggta
     1921 ccgccctttc ctaatgacag cggctctgcg attggtgcag cctgctgtga aatagtggcc
     1981 cagcaagggt tcgtgccatt agactggtca gtctacagcg gcccgagcct gcaagacagg
     2041 caagtaccag ctgggtggca tgctagcccg tgcagcatct cggaagtggc cgccatcctc
     2101 gccagcaaca aacccgtggt tttcctttcc gggcgcacgg agcttggacc acgggcactg
     2161 ggaggaagaa gcattctggc ggctgcgacc tcgccggaaa tgaaagatca tcttaacgaa
     2221 atcaaattcc gcgaacactt ccggccagtt gcgccaatat gtctggagga ccgtgcaccg
     2281 gatatattta gtcccggaac ccccgatcca tacatgttgt tcgatcacca gacgaagatg
     2341 ccgtggcagg acaaagttcc tgcagttgta catcttgacg gatctgcgcg gctgcaaacc
     2401 atttctagga actctcagca taaggtcgcc gaggtacttg tcgaatatga aaagctcacg
     2461 ggcattccgt tgctttgcaa cacaagtgcc aattaccatg ggcgtggctt cttcccaagt
     2521 gcagccgcag cctgcgagtg gggccgcgtt gaacacgtat ggtgcgacgg catgctatac
     2581 aggaaaccgt ccgcaaccgc gtgatcctat cgggcgcggg cttcgcgaag atacgtgtat
     2641 gtcaacgcat aaggttgctg ccccacggcg aaaggtcact cttccactgg ggagcagcga
     2701 act
//