From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Venezuelan equine encephalitis virus mRNA.
Date: 11 Sep 92 10:03:41 GMT
Distribution: bionet
Checksum: 13609    23

LOCUS       EEVNSPEPB   11446 bp ss-mRNA            VRL       11-SEP-1992
DEFINITION  Venezuelan equine encephalitis virus mRNA.
ACCESSION   L01443
KEYWORDS    capsid protein; core protein; envelope glycoprotein;
            fusion protein; intracellular; nonstructural protein.
SOURCE      Venezuelan equine encephalitis virus (strain TC-83) cDNA to mRNA.
  ORGANISM  Venezuelan equine encephalitis virus
            Viridae; ss-RNA enveloped viruses; Positive strand RNA virus;
            Togaviridae; Alphaviridae.
REFERENCE   1  (bases 1 to 11446)
  AUTHORS   Johnson,B.J., Kinney,R.M., Kost,C.L. and Trent,D.W.
  TITLE     Molecular determinants of alphavirus neurovirulence: Nucleotide and
            deduced protein sequence changes during attenuation of Venezuelan
            equine encephalitis virus
  JOURNAL   J. Gen. Virol. 67, 1951-1960 (1986)
  STANDARD  full staff_entry
REFERENCE   2  (bases 1 to 11446)
  AUTHORS   Kinney,R.M., Johnson,B.J., Welch,J.B., Tsuchiya,K.R. and Trent,D.W.
  TITLE     The full-length nucleotide sequences of the virulent Trinidad
            donkey strain of Venezuelan equine encephalitis virus and its
            attenuated vaccine derivative. strain TC-83
  JOURNAL   Virology 170, 19-30 (1989)
  STANDARD  full staff_entry
FEATURES             Location/Qualifiers
     5'UTR           1..44
     mRNA            1..11446
                     /function="positive-sense RNA genome of VEE TC-83 virus"
                     /note="putative"
     sig_peptide     45..1649
                     /gene="nsP1"
                     /note="putative"
                     /codon_start=1
     CDS             45..7526
                     /function="replication"
                     /note="alpha virus nonstructural proteins are derived by
                     intracellular cleavage of polyprotein precursors
                     translated from the 5'-two-thirds portion of the genomic
                     mRNA"
                     /product="nonstructural polyprotein"
                     /gene="nsP1"
                     /note="putative"
                     /codon_start=1
                     /translation="MEKVHVDIEEDSPFLRALQRSFPQFEVEAKQVTDNDHANARAFS
                     HLASKLIETEVDPSDTILDIGSAPARRMYSKHKYHCICPMRCAEDPDRLYKYATKLKK
                     NCKEITDKELDKKMKELAAVMSDPDLETETMCLHDDESCRYEGQVAVYQDVYAVDGPT
                     SLYHQANKGVRVAYWIGFDTTPFMFKNLAGAYPSYSTNWADETVLTARNIGLCSSDVM
                     ERSRRGMSILRKKYLKPSNNVLFSVGSTIYHEKRDLLRSWHLPSVFHLRGKQNYTCRC
                     ETIVSCDGYVVKRIAISPGLYGKPSGYAATMHREGFLCCKVTDTLNGERVSFPVCTYV
                     PATLCDQMTGILATDVSADDAQKLLVGLNQRIVVNGRTQRNTNTMKNYLLPVVAQAFA
                     RWAKEYKEDQEDERPLGLRDRQLVMGCCWAFRRHKITSIYKRPDTQTIIKVNSDFHSF
                     VLPRIGSNTLEIGLRTRIRKMLEEHKEPSPLITAEDVQEAKCAADEAKEVREAEELRA
                     ALPPLAADVEEPTLEADVDLMLQEAGAGSVETPRGLIKVTSYDGEDKIGSYAVLSPQA
                     VLKSEKLSCIHPLAEQVIVITHSGRKGRYAVEPYHGKVVVPEGHAIPVQDFQALSESA
                     TIVYNEREFVNRYLHHIATHGGALNTDEEYYKTVKPSEHDGEYLYDIDRKQCVKKELV
                     TGLGLTGELVDPPFHEFAYESLRTRPAAPYQVPTIGVYGVPGSGKSGIIKSAVTKKDL
                     VVSAKKENCAEIIRDVKKMKGLDVNARTVDSVLLNGCKHPVETLYIDEAFACHAGTLR
                     ALIAIIRPKKAVLCGDPKQCGFFNMMCLKVHFNHEICTQVFHKSISRRCTKSVTSVVS
                     TLFYDKKMRTTNPKETKIVIDTTGSTKPKQDDLILTCFRGWVKQLQIDYKGNEIMTAA
                     ASQGLTRKGVYAVRYKVNENPLYAPTSEHVNVLLTRTEDRIVWKTLAGDPWIKTLTAK
                     YPGNFTATIEEWQAEHDAIMRHILERPDPTDVFQNKANVCWAKALVPVLKTAGIDMTT
                     EQWNTVDYFETDKAHSAEIVLNQLCVRFFGLDLDSGLFSAPTVPLSIRNNHWDNSPSP
                     NMYGLNKEVVRQLSRRYPQLPRAVATGRVYDMNTGTLRNYDPRINLVPVNRRLPHALV
                     LHHNEHPQSDFSSFVSKLKGRTVLVVGEKLSVPGKMVDWLSDRPEATFRARLDLGIPG
                     DVPKYDIIFVNVRTPYKYHHYQQCEDHAIKLSMLTKKACLHLNPGGTCVSIGYGYADR
                     ASESIIGAIARQFKFSRVCKPKSSLEETEVLFVFIGYDRKARTHNPYKLSSTLTNIYT
                     GSRLHEAGCAPSYHVVRGDIATATEGVIINAANSKGQPGGGVCGALYKKFPESFDLQP
                     IEVGKARLVKGAAKHIIHAVGPNFNKVSEVEGDKQLAEAYESIAKIVNDNNYKSVAIP
                     LLSTGIFSGNKDRLTQSLNHLLTALDTTDADVAIYCRDKKWEMTLKEAVARREAVEEI
                     CISDDSSVTEPDAELVRVHPKSSLAGRKGYSTSDGKTFSYLEGTKFHQAAKDIAEINA
                     MWPVATEANEQVCMYILGESMSSIRSKCPVEESEASTPPSTLPCLCIHAMTPERVQRL
                     KASRPEQITVCSSFPLPKYRITGVQKIQCSQPILFSPKVPAYIHPRKYLVETPPVDET
                     PEPSAENQSTEGTPEQPPLITEDETRTRTPEPIIIEEEEEDSISLLSDGPTHQVLQVE
                     ADIHGPPSVSSSSWSIPHASDFDVDSLSILDTLEGASVTSGATSAETNSYFAKSMEFL
                     ARPVPAPRTVFRNPPHPAPRTRTPSLAPSRACSRTSLVSTPPGVNRVITREELEALTP
                     SRTPSRSVSRTSLVSNPPGVNRVITREEFEAFVAQQQXRFDAGAYIFSSDTGQGHLQQ
                     KSVRQTVLSEVVLERTELEISYAPRLDQEKEELLRKKLQLNPTPANRSRYQSRKVENM
                     KAITARRILQGLGHYLKAEGKVECYRTLHPVPLYSSSVNRAFSSPKVAVEACNAMLKE
                     NFPTVASYCIIPEYDAYLDMVDGASCCLDTASFCPAKLRSFPKKHSYLEPTIRSAVPS
                     AIQNTLQNVLAAATKRNCNVTQMRELPVLDSAAFNVECFKKYACNNEYWETFKENPIR
                     LTEENVVNYITKLKGPKAAALFAKTHNLNMLQDIPMDRFVMDLKRDVKVTPGTKHTEE
                     RPKVQVIQAADPLATAYLCGIHRELVRRLNAVLLPNIHTLFDMSAEDFDAIIAEHFQP
                     GDCVLETDIASFDKSEDDAMALTALMILEDLGVDAELLTLIEAAFGEISSIHLPTKTK
                     FKFGAMMKSGMFLTLFVNTVINIVIASRVLRERLTGSPCAAFIGDDNIVKGVKSDKLM
                     ADRCATWLNMEVKIIDAVVGEKAPYFCGGFILCDSVTGTACRVADPLKRLFKLGKPLA
                     ADDEHDDDRRRALHEESTRWNRVGILSELCKAVESRYETVGTSIIVMAMTTLASSVKS
                     FSYLRGAPITLYG"
     old_sequence    replace(106..107,"gc")
                     /note="this correction results in changing amino acid
                     nsP1-21-Thr to nsP1-21-Ser, this 1992 data corrects the
                     sequence reported in the reference in citation [1]"
                     /citation=[1]
                     /citation=[2]
     old_sequence    replace(1533..1534,"gc")
                     /note="this correction changes amino acid nsP1-497-Arg to
                     nsP1-497-Ala, this data corrects the sequence reported in
                     the reference in citation [1]"
                     /citation=[1]
                     /citation=[2]
     exon            1650..4031
                     /function="replication"
                     /gene="nsP2"
                     /note="putative"
     exon            5703..7523
                     /function="replication"
                     /gene="nsP4"
                     /note="putative"
     mRNA            7532..11446
                     /function="encodes the viral structural proteins"
                     /note="the structural proteins of alpha viruses are
                     translated as a polyprotein precursor from an
                     intracellular 26S mRNA species that is identical to the
                     3'-one-third portion of the viral genomic mRNA"
                     /gene="26S mRNA"
                     /note="putative"
     CDS             7562..8386
                     /function="contains the genomic RNA in the mature virion"
                     /standard_name="capsid protein"
                     /product="core protein"
                     /note="putative"
                     /codon_start=1
                     /translation="MFPFQPMYPMQPMPYRNPFAAPRRPWFPRTDPFLAMQVQELTRS
                     MANLTFKQRRDAPPEGPSAKKPKKEASQKQKGGGQGKKKKNQGKKKAKTGPPNPKAQN
                     GNKKKTNKKPGKRQRMVMKLESDKTFPIMLEGKINGYACVVGGKLFRPMHVEGKIDND
                     VLAALKTKKASKYDLEYADVPQNMRADTFKYTHEKPQGYYSWHHGAVQYENGRFTVPK
                     GVGAKGDSGRPILDNQGRVVAIVLGGVNEGSRTALSVVMWNEKGVTVKYTPENCEQW"
                     /partial
     exon            8387..8563
                     /function="icorporated into the envelope of some alpha
                     viruses"
                     /gene="E3"
                     /note="putative"
     exon            8564..9832
                     /function="cell attachment, elicits virus neutralizing
                     antibodies"
                     /gene="E2"
                     /note="putative"
     exon            9833..10000
                     /standard_name="6K protein"
                     /note="putative"
     old_sequence    replace(9989..9991,"ggc")
                     /note="this correction results in an insertion of an extra
                     'ggc' codon (Gly) between the old genome sequence
                     positions 9988 and 9989 in the reference in citation
                     [1][2]"
                     /citation=[1][2]
                     /citation=[2]
                     /citation=[1]
     exon            10001..11326
                     /function="fusion protein, immunogenicity"
                     /gene="E1"
                     /note="putative"
     3'UTR           11327..11446
     polyA_site      11446
BASE COUNT     3234 a   2800 c   2895 g   2517 t
ORIGIN
        1 ataggcggcg catgagagaa gcccagacca attacctacc caaaatggag aaagttcacg
       61 ttgacatcga ggaagacagc ccattcctca gagctttgca gcggagcttc ccgcagtttg
      121 aggtagaagc caagcaggtc actgataatg accatgctaa tgccagagcg ttttcgcatc
      181 tggcttcaaa actgatcgaa acggaggtgg acccatccga cacgatcctt gacattggaa
      241 gtgcgcccgc ccgcagaatg tattctaagc acaagtatca ttgtatctgt ccgatgagat
      301 gtgcggaaga tccggacaga ttgtataagt atgcaactaa gctgaagaaa aactgtaagg
      361 aaataactga taaggaattg gacaagaaaa tgaaggagct cgccgccgtc atgagcgacc
      421 ctgacctgga aactgagact atgtgcctcc acgacgacga gtcgtgtcgc tacgaagggc
      481 aagtcgctgt ttaccaggat gtatacgcgg ttgacggacc gacaagtctc tatcaccaag
      541 ccaataaggg agttagagtc gcctactgga taggctttga caccacccct tttatgttta
      601 agaacttggc tggagcatat ccatcatact ctaccaactg ggccgacgaa accgtgttaa
      661 cggctcgtaa cataggccta tgcagctctg acgttatgga gcggtcacgt agagggatgt
      721 ccattcttag aaagaagtat ttgaaaccat ccaacaatgt tctattctct gttggctcga
      781 ccatctacca cgagaagagg gacttactga ggagctggca cctgccgtct gtatttcact
      841 tacgtggcaa gcaaaattac acatgtcggt gtgagactat agttagttgc gacgggtacg
      901 tcgttaaaag aatagctatc agtccaggcc tgtatgggaa gccttcaggc tatgctgcta
      961 cgatgcaccg cgagggattc ttgtgctgca aagtgacaga cacattgaac ggggagaggg
     1021 tctcttttcc cgtgtgcacg tatgtgccag ctacattgtg tgaccaaatg actggcatac
     1081 tggcaacaga tgtcagtgcg gacgacgcgc aaaaactgct ggttgggctc aaccagcgta
     1141 tagtcgtcaa cggtcgcacc cagagaaaca ccaataccat gaaaaattac cttttgcccg
     1201 tagtggccca ggcatttgct aggtgggcaa aggaatataa ggaagatcaa gaagatgaaa
     1261 ggccactagg actacgagat agacagttag tcatggggtg ttgttgggct tttagaaggc
     1321 acaagataac atctatttat aagcgcccgg atacccaaac catcatcaaa gtgaacagcg
     1381 atttccactc attcgtgctg cccaggatag gcagtaacac attggagatc gggctgagaa
     1441 caagaatcag gaaaatgtta gaggagcaca aggagccgtc acctctcatt accgccgagg
     1501 acgtacaaga agctaagtgc gcagccgatg aggctaagga ggtgcgtgaa gccgaggagt
     1561 tgcgcgcagc tctaccacct ttggcagctg atgttgagga gcccactctg gaagccgatg
     1621 tcgacttgat gttacaagag gctggggccg gctcagtgga gacacctcgt ggcttgataa
     1681 aggttaccag ctacgatggc gaggacaaga tcggctctta cgctgtgctt tctccgcagg
     1741 ctgtactcaa gagtgaaaaa ttatcttgca tccaccctct cgctgaacaa gtcatagtga
     1801 taacacactc tggccgaaaa gggcgttatg ccgtggaacc ataccatggt aaagtagtgg
     1861 tgccagaggg acatgcaata cccgtccagg actttcaagc tctgagtgaa agtgccacca
     1921 ttgtgtacaa cgaacgtgag ttcgtaaaca ggtacctgca ccatattgcc acacatggag
     1981 gagcgctgaa cactgatgaa gaatattaca aaactgtcaa gcccagcgag cacgacggcg
     2041 aatacctgta cgacatcgac aggaaacagt gcgtcaagaa agaactagtc actgggctag
     2101 ggctcacagg cgagctggtg gatcctccct tccatgaatt cgcctacgag agtctgagaa
     2161 cacgaccagc cgctccttac caagtaccaa ccataggggt gtatggcgtg ccaggatcag
     2221 gcaagtctgg catcattaaa agcgcagtca ccaaaaaaga tctagtggtg agcgccaaga
     2281 aagaaaactg tgcagaaatt ataagggacg tcaagaaaat gaaagggctg gacgtcaatg
     2341 ccagaactgt ggactcagtg ctcttgaatg gatgcaaaca ccccgtagag accctgtata
     2401 ttgacgaagc ttttgcttgt catgcaggta ctctcagagc gctcatagcc attataagac
     2461 ctaaaaaggc agtgctctgc ggggatccca aacagtgcgg tttttttaac atgatgtgcc
     2521 tgaaagtgca ttttaaccac gagatttgca cacaagtctt ccacaaaagc atctctcgcc
     2581 gttgcactaa atctgtgact tcggtcgtct caaccttgtt ttacgacaaa aaaatgagaa
     2641 cgacgaatcc gaaagagact aagattgtga ttgacactac cggcagtacc aaacctaagc
     2701 aggacgatct cattctcact tgtttcagag ggtgggtgaa gcagttgcaa atagattaca
     2761 aaggcaacga aataatgacg gcagctgcct ctcaagggct gacccgtaaa ggtgtgtatg
     2821 ccgttcggta caaggtgaat gaaaatcctc tgtacgcacc cacctcagaa catgtgaacg
     2881 tcctactgac ccgcacggag gaccgcatcg tgtggaaaac actagccggc gacccatgga
     2941 taaaaacact gactgccaag taccctggga atttcactgc cacgatagag gagtggcaag
     3001 cagagcatga tgccatcatg aggcacatct tggagagacc ggaccctacc gacgtcttcc
     3061 agaataaggc aaacgtgtgt tgggccaagg ctttagtgcc ggtgctgaag accgctggca
     3121 tagacatgac cactgaacaa tggaacactg tggattattt tgaaacggac aaagctcact
     3181 cagcagagat agtattgaac caactatgcg tgaggttctt tggactcgat ctggactccg
     3241 gtctattttc tgcacccact gttccgttat ccattaggaa taatcactgg gataactccc
     3301 cgtcgcctaa catgtacggg ctgaataaag aagtggtccg tcagctctct cgcaggtacc
     3361 cacaactgcc tcgggcagtt gccactggaa gagtctatga catgaacact ggtacactgc
     3421 gcaattatga tccgcgcata aacctagtac ctgtaaacag aagactgcct catgctttag
     3481 tcctccacca taatgaacac ccacagagtg acttttcttc attcgtcagc aaattgaagg
     3541 gcagaactgt cctggtggtc ggggaaaagt tgtccgtccc aggcaaaatg gttgactggt
     3601 tgtcagaccg gcctgaggct accttcagag ctcggctgga tttaggcatc ccaggtgatg
     3661 tgcccaaata tgacataata tttgttaatg tgaggacccc atataaatac catcactatc
     3721 agcagtgtga agaccatgcc attaagctta gcatgttgac caagaaagct tgtctgcatc
     3781 tgaatcccgg cggaacctgt gtcagcatag gttatggtta cgctgacagg gccagcgaaa
     3841 gcatcattgg tgctatagcg cggcagttca agttttcccg ggtatgcaaa ccgaaatcct
     3901 cacttgaaga gacggaagtt ctgtttgtat tcattgggta cgatcgcaag gcccgtacgc
     3961 acaatcctta caagctttca tcaaccttga ccaacattta tacaggttcc agactccacg
     4021 aagccggatg tgcaccctca tatcatgtgg tgcgagggga tattgccacg gccaccgaag
     4081 gagtgattat aaatgctgct aacagcaaag gacaacctgg cggaggggtg tgcggagcgc
     4141 tgtataagaa attcccggaa agcttcgatt tacagccgat cgaagtagga aaagcgcgac
     4201 tggtcaaagg tgcagctaaa catatcattc atgccgtagg accaaacttc aacaaagttt
     4261 cggaggttga aggtgacaaa cagttggcag aggcttatga gtccatcgct aagattgtca
     4321 acgataacaa ttacaagtca gtagcgattc cactgttgtc caccggcatc ttttccggga
     4381 acaaagatcg actaacccaa tcattgaacc atttgctgac agctttagac accactgatg
     4441 cagatgtagc catatactgc agggacaaga aatgggaaat gactctcaag gaagcagtgg
     4501 ctaggagaga agcagtggag gagatatgca tatccgacga ctcttcagtg acagaacctg
     4561 atgcagagct ggtgagggtg catccgaaga gttctttggc tggaaggaag ggctacagca
     4621 caagcgatgg caaaactttc tcatatttgg aagggaccaa gtttcaccag gcggccaagg
     4681 atatagcaga aattaatgcc atgtggcccg ttgcaacgga ggccaatgag caggtatgca
     4741 tgtatatcct cggagaaagc atgagcagta ttaggtcgaa atgccccgtc gaagagtcgg
     4801 aagcctccac accacctagc acgctgcctt gcttgtgcat ccatgccatg actccagaaa
     4861 gagtacagcg cctaaaagcc tcacgtccag aacaaattac tgtgtgctca tcctttccat
     4921 tgccgaagta tagaatcact ggtgtgcaga agatccaatg ctcccagcct atattgttct
     4981 caccgaaagt gcctgcgtat attcatccaa ggaagtatct cgtggaaaca ccaccggtag
     5041 acgagactcc ggagccatcg gcagagaacc aatccacaga ggggacacct gaacaaccac
     5101 cacttataac cgaggatgag accaggacta gaacgcctga gccgatcatc atcgaagagg
     5161 aagaagagga tagcataagt ttgctgtcag atggcccgac ccaccaggtg ctgcaagtcg
     5221 aggcagacat tcacgggccg ccctctgtat ctagctcatc ctggtccatt cctcatgcat
     5281 ccgactttga tgtggacagt ttatccatac ttgacaccct ggagggagct agcgtgacca
     5341 gcggggcaac gtcagccgag actaactctt acttcgcaaa gagtatggag tttctggcgc
     5401 gaccggtgcc tgcgcctcga acagtattca ggaaccctcc acatcccgct ccgcgcacaa
     5461 gaacaccgtc acttgcaccc agcagggcct gctcgagaac cagcctagtt tccaccccgc
     5521 caggcgtgaa tagggtgatc actagagagg agctcgaggc gcttaccccg tcacgcactc
     5581 ctagcaggtc ggtctcgaga accagcctgg tctccaaccc gccaggcgta aatagggtga
     5641 ttacaagaga ggagtttgag gcgttcgtag cacaacaaca atgacggttt gatgcgggtg
     5701 catacatctt ttcctccgac accggtcaag ggcatttaca acaaaaatca gtaaggcaaa
     5761 cggtgctatc cgaagtggtg ttggagagga ccgaattgga gatttcgtat gccccgcgcc
     5821 tcgaccaaga aaaagaagaa ttactacgca agaaattaca gttaaatccc acacctgcta
     5881 acagaagcag ataccagtcc aggaaggtgg agaacatgaa agccataaca gctagacgta
     5941 ttctgcaagg cctagggcat tatttgaagg cagaaggaaa agtggagtgc taccgaaccc
     6001 tgcatcctgt tcctttgtat tcatctagtg tgaaccgtgc cttttcaagc cccaaggtcg
     6061 cagtggaagc ctgtaacgcc atgttgaaag agaactttcc gactgtggct tcttactgta
     6121 ttattccaga gtacgatgcc tatttggaca tggttgacgg agcttcatgc tgcttagaca
     6181 ctgccagttt ttgccctgca aagctgcgca gctttccaaa gaaacactcc tatttggaac
     6241 ccacaatacg atcggcagtg ccttcagcga tccagaacac gctccagaac gtcctggcag
     6301 ctgccacaaa aagaaattgc aatgtcacgc aaatgagaga attgcccgta ttggattcgg
     6361 cggcctttaa tgtggaatgc ttcaagaaat atgcgtgtaa taatgaatat tgggaaacgt
     6421 ttaaagaaaa ccccatcagg cttactgaag aaaacgtggt aaattacatt accaaattaa
     6481 aaggaccaaa agctgctgct ctttttgcga agacacataa tttgaatatg ttgcaggaca
     6541 taccaatgga caggtttgta atggacttaa agagagacgt gaaagtgact ccaggaacaa
     6601 aacatactga agaacggccc aaggtacagg tgatccaggc tgccgatccg ctagcaacag
     6661 cgtatctgtg cggaatccac cgagagctgg ttaggagatt aaatgcggtc ctgcttccga
     6721 acattcatac actgtttgat atgtcggctg aagactttga cgctattata gccgagcact
     6781 tccagcctgg ggattgtgtt ctggaaactg acatcgcgtc gtttgataaa agtgaggacg
     6841 acgccatggc tctgaccgcg ttaatgattc tggaagactt aggtgtggac gcagagctgt
     6901 tgacgctgat tgaggcggct ttcggcgaaa tttcatcaat acatttgccc actaaaacta
     6961 aatttaaatt cggagccatg atgaaatctg gaatgttcct cacactgttt gtgaacacag
     7021 tcattaacat tgtaatcgca agcagagtgt tgagagaacg gctaaccgga tcaccatgtg
     7081 cagcattcat tggagatgac aatatcgtga aaggagtcaa atcggacaaa ttaatggcag
     7141 acaggtgcgc cacctggttg aatatggaag tcaagattat agatgctgtg gtgggcgaga
     7201 aagcgcctta tttctgtgga gggtttattt tgtgtgactc cgtgaccggc acagcgtgcc
     7261 gtgtggcaga ccccctaaaa aggctgttta agcttggcaa acctctggca gcagacgatg
     7321 aacatgatga tgacaggaga agggcattgc atgaagagtc aacacgctgg aaccgagtgg
     7381 gtattctttc agagctgtgc aaggcagtag aatcaaggta tgaaaccgta ggaacttcca
     7441 tcatagttat ggccatgact actctagcta gcagtgttaa atcattcagc tacctgagag
     7501 gggcccctat aactctctac ggctaacctg aatggactac gacatagtct agtccgccaa
     7561 gatgttcccg ttccagccaa tgtatccgat gcagccaatg ccctatcgca acccgttcgc
     7621 ggccccgcgc aggccctggt tccccagaac cgaccctttt ctggcgatgc aggtgcagga
     7681 attaacccgc tcgatggcta acctgacgtt caagcaacgc cgggacgcgc cacctgaggg
     7741 gccatccgct aagaaaccga agaaggaggc ctcgcaaaaa cagaaagggg gaggccaagg
     7801 gaagaagaag aagaaccaag ggaagaagaa ggctaagaca gggccgccta atccgaaggc
     7861 acagaatgga aacaagaaga agaccaacaa gaaaccaggc aagagacagc gcatggtcat
     7921 gaaattggaa tctgacaaga cgttcccaat catgttggaa gggaagataa acggctacgc
     7981 ttgtgtggtc ggagggaagt tattcaggcc gatgcatgtg gaaggcaaga tcgacaacga
     8041 cgttctggcc gcgcttaaga cgaagaaagc atccaaatac gatcttgagt atgcagatgt
     8101 gccacagaac atgcgggccg atacattcaa atacacccat gagaaacccc aaggctatta
     8161 cagctggcat catggagcag tccaatatga aaatgggcgt ttcacggtgc cgaaaggagt
     8221 tggggccaag ggagacagcg gacgacccat tctggataac cagggacggg tggtcgctat
     8281 tgtgctggga ggtgtgaatg aaggatctag gacagccctt tcagtcgtca tgtggaacga
     8341 gaagggagtt accgtgaagt atactccgga gaactgcgag caatggtcac tagtgaccac
     8401 catgtgtctg ctcgccaatg tgacgttccc atgtgctcaa ccaccaattt gctacgacag
     8461 aaaaccagca gagactttgg ccatgctcag cgttaacgtt gacaacccgg gctacgatga
     8521 gctgctggaa gcagctgtta agtgccccgg aaggaaaagg agatccaccg aggagctgtt
     8581 taatgagtat aagctaacgc gcccttacat ggccagatgc atcagatgtg cagttgggag
     8641 ctgccatagt ccaatagcaa tcgaggcagt aaagagcgac gggcacgacg gttatgttag
     8701 acttcagact tcctcgcagt atggcctgga ttcctccggc aacttaaagg gcaggaccat
     8761 gcggtatgac atgcacggga ccattaaaga gataccacta catcaagtgt cactctatac
     8821 atctcgcccg tgtcacattg tggatgggca cggttatttc ctgcttgcca ggtgcccggc
     8881 aggggactcc atcaccatgg aatttaagaa agattccgtc agacactcct gctcggtgcc
     8941 gtatgaagtg aaatttaatc ctgtaggcag agaactctat actcatcccc cagaacacgg
     9001 agtagagcaa gcgtgccaag tctacgcaca tgatgcacag aacagaggag cttatgtcga
     9061 gatgcacctc ccgggctcag aagtggacag cagtttggtt tccttgagcg gcagttcagt
     9121 caccgtgaca cctcctgatg ggactagcgc cctggtggaa tgcgagtgtg gcggcacaaa
     9181 gatctccgag accatcaaca agacaaaaca gttcagccag tgcacaaaga aggagcagtg
     9241 cagagcatat cggctgcaga acgataagtg ggtgtataat tctgacaaac tgcccaaagc
     9301 agcgggagcc accttaaaag gaaaactgca tgtcccattc ttgctggcag acggcaaatg
     9361 caccgtgcct ctagcaccag aacctatgat aaccttcggt ttcagatcag tgtcactgaa
     9421 actgcaccct aagaatccca catatctaat cacccgccaa cttgctgatg agcctcacta
     9481 cacgcacgag ctcatatctg aaccagctgt taggaatttt accgtcaccg aaaaagggtg
     9541 ggagtttgta tggggaaacc acccgccgaa aaggttttgg gcacaggaaa cagcacccgg
     9601 aaatccacat gggctaccgc acgaggtgat aactcattat taccacagat accctatgtc
     9661 caccatcctg ggtttgtcaa tttgtgccgc cattgcaacc gtttccgttg cagcgtctac
     9721 ctggctgttt tgcagatcta gagttgcgtg cctaactcct taccggctaa cacctaacgc
     9781 taggatacca ttttgtctgg ctgtgctttg ctgcgcccgc actgcccggg ccgagaccac
     9841 ctgggagtcc ttggatcacc tatggaacaa taaccaacag atgttctgga ttcaattgct
     9901 gatccctctg gccgccttga tcgtagtgac tcgcctgctc aggtgcgtgt gctgtgtcgt
     9961 gcctttttta gtcatggccg gcgccgcagg cgccggcgcc tacgagcacg cgaccacgat
    10021 gccgagccaa gcgggaatct cgtataacac tatagtcaac agagcaggct acgcaccact
    10081 ccctatcagc ataacaccaa caaagatcaa gctgatacct acagtgaact tggagtacgt
    10141 cacctgccac tacaaaacag gaatggattc accagccatc aaatgctgcg gatctcagga
    10201 atgcactcca acttacaggc ctgatgaaca gtgcaaagtc ttcacagggg tttacccgtt
    10261 catgtggggt ggtgcatatt gcttttgcga cactgagaac acccaagtca gcaaggccta
    10321 cgtaatgaaa tctgacgact gccttgcgga tcatgctgaa gcatataaag cgcacacagc
    10381 ctcagtgcag gcgttcctca acatcacagt gggagaacac tctattgtga ctaccgtgta
    10441 tgtgaatgga gaaactcctg tgaatttcaa tggggtcaaa ataactgcag gtccgctttc
    10501 cacagcttgg acaccctttg atcgcaaaat cgtgcagtat gccggggaga tctataatta
    10561 tgattttcct gagtatgggg caggacaacc aggagcattt ggagatatac aatccagaac
    10621 agtctcaagc tctgatctgt atgccaatac caacctagtg ctgcagagac ccaaagcagg
    10681 agcgatccac gtgccataca ctcaggcacc ttcgggtttt gagcaatgga agaaagataa
    10741 agctccatca ttgaaattta ccgccccttt cggatgcgaa atatatacaa accccattcg
    10801 cgccgaaaac tgtgctgtag ggtcaattcc attagccttt gacattcccg acgccttgtt
    10861 caccagggtg tcagaaacac cgacactttc agcggccgaa tgcactctta acgagtgcgt
    10921 gtattcttcc gactttggtg ggatcgccac ggtcaagtac tcggccagca agtcaggcaa
    10981 gtgcgcagtc catgtgccat cagggactgc taccctaaaa gaagcagcag tcgagctaac
    11041 cgagcaaggg tcggcgacta tccatttctc gaccgcaaat atccacccgg agttcaggct
    11101 ccaaatatgc acatcatatg ttacgtgcaa aggtgattgt caccccccga aagaccatat
    11161 tgtgacacac cctcagtatc acgcccaaac atttacagcc gcggtgtcaa aaaccgcgtg
    11221 gacgtggtta acatccctgc tgggaggatc agccgtaatt attataattg gcttggtgct
    11281 ggctactatt gtggccatgt acgtgctgac caaccagaaa cataattgaa tacagcagca
    11341 attggcaagc tgcttacata gaactcgcgg cgattggcat gccgccttaa aatttttatt
    11401 ttatttttct tttcttttcc gaatcggatt ttgtttttaa tatttc
//