From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Simian (macaque) immunodeficiency virus proviral DNA.
Date: 24 Jul 92 10:10:02 GMT
Distribution: bionet
Checksum: 15393    25

LOCUS       SIVMM32H    10277 bp ds-DNA             VRL       24-JUL-1992
DEFINITION  Simian (macaque) immunodeficiency virus proviral DNA.
ACCESSION   D01065
KEYWORDS    env polyprotein; gag polyprotein; nef protein; pol polyprotein;
            rev protein; tat protein; vif protein; vpr protein; vpx protein.
SOURCE      Simian (macaque) immunodeficiency virus proviral DNA, SIVmac32H
            isolate, propagated in human T-cell (cell line JM), clone pJ5.
  ORGANISM  Simian immunodeficiency virus
            Viridae; ss-RNA enveloped viruses; Positive strand RNA virus;
            Retroviridae; Lentivirinae.
REFERENCE   1  (bases 1 to 10277)
  AUTHORS   Rud,E.W., Yon,J.R., Cranage,M.P., Larder,B.A. and Clarke,B.E.
  TITLE     Molecular cloning and sequence of the simian immunodeficiency
            virus, SIVmac32H
  JOURNAL   Unpublished (1991)
  STANDARD  full staff_review
REFERENCE   2  (sites)
  AUTHORS   Rud,E.W., Yon,J.R., Larder,B.A., Cook,N.J., Cranage,M.P. and
            Clarke,B.E.
  TITLE     Infectious molecular clones of SIVmac32H: Nef deletion controls
            ability to reisolate virus from rhesus macaques
  JOURNAL   Vaccine (1992) In press
  STANDARD  full staff_review
COMMENT     Submitted (10-JUN-1991) to DDBJ by:
            Erling W Rud
            Molecular Sciences
            Wellcome Research Laboratories
            Langley Court, South Eden Park Road
            Beckenham, Kent BR3 3BS
            U.K.
            Phone:  081-658-2211 x5279
            Fax:    081-663-3532
            SIVmac32H proviral clone pJ5 contains no premature stop codons in
            any of the known SIV open reading frames. The variant pC8 is
            identical to pJ5 except for the changes indicated in the feature
            table.
            [2]  sites.
FEATURES             Location/Qualifiers
     exon            <6303..6598
                     /number=1
                     /product="tat protein"
     exon            <6529..6598
                     /number=1
                     /product="rev protein"
     CDS             join(6303..6598,8801..8900)
                     /function="transactivation"
                     /product="tat protein"
                     /gene="tat"
                     /codon_start=1
                     /translation="METPLREQENSLESSNERSSCISEADATTPESANLGEEILSQLY
                     RPLEACYNTCYCKKCCYHCQFCFLKKGLGICYEQSRKRRRTPKKAKANTSSASNNRPI
                     SNRTRHCQPEKAKKETVEKAVATAPGLGR"
     CDS             join(6529..6598,8801..9057)
                     /product="rev protein"
                     /gene="rev"
                     /codon_start=1
                     /translation="MSSHEREEELRKRLRLIHLLHQTTDPYPTGPGTANQRRQRRRRW
                     RRRWQQLLALADRIYSFPDPPTDTPLDLAIQQLQNLAIESIPDPPTNTPEALCGPTEN
                     SRSPQD"
     CDS             join(6303..6598,8804..8900)
                     /note="alternate"
                     /product="tat protein"
                     /gene="tat"
                     /codon_start=1
                     /translation="METPLREQENSLESSNERSSCISEADATTPESANLGEEILSQLY
                     RPLEACYNTCYCKKCCYHCQFCFLKKGLGICYEQSRKRRRTPKKAKANTSSASNKPIS
                     NRTRHCQPEKAKKETVEKAVATAPGLGR"
     CDS             join(6529..6598,8804..9057)
                     /note="alternate"
                     /product="rev protein"
                     /gene="rev"
                     /codon_start=1
                     /translation="MSSHEREEELRKRLRLIHLLHQTNPYPTGPGTANQRRQRRRRWR
                     RRWQQLLALADRIYSFPDPPTDTPLDLAIQQLQNLAIESIPDPPTNTPEALCGPTENS
                     RSPQD"
     LTR             1..818
                     /note="5'LTR"
     LTR             1..518
                     /note="5'LTR U3 region"
     protein_bind    415..424
                     /bound_moiety="NFkB"
     protein_bind    429..438
                     /bound_moiety="Sp1"
                     /note="4"
     protein_bind    440..449
                     /bound_moiety="Sp1"
                     /note="3"
     protein_bind    451..460
                     /bound_moiety="Sp1"
                     /note="2"
     protein_bind    462..471
                     /bound_moiety="Sp1"
                     /note="1"
     TATA_signal     488..494
     misc_feature    518..519
                     /note="put. mRNA start site"
     repeat_unit     519..694
                     /rpt_type=terminal
     LTR             695..818
                     /note="5'LTR U5 region"
     primer_bind     822..849
                     /note="tRNA (Lys) primer binding site"
     CDS             1054..2586
                     /product="gag polyprotein"
                     /gene="gag"
                     /codon_start=1
                     /translation="MGARNSVLSGKKADELEKIRLRPGGKKKYMLKHVVWAANELDRF
                     GLAESLLENKEGCQKILSVLAPLVPTGSENLKSLYNTVCVIWCIHAEEKVKHTEEAKQ
                     IVQRHLVVETGTAETMPKTSRPTAPSSGRGGNYPVQQIGGNYVHLPLSPRTLNAWVKL
                     IEEKKFGAEVVPGFQALSEGCTPYDINQMLNCVGDHQAAMQIIRDIINEEAADWDLQH
                     PQPAPQQGQLREPSGSDIAGTTSSVDEQIQWMYRQQNPIPVGNIYRRWIQLGLQKCVR
                     MYNPTNILDVKQGPKEPFQSYVDRFYKSLRAEQTDAAVKNWMTQTLLIQNANPDCKLV
                     LKGLGVNPTLEEMLTACQGVGGPGQKARLMAEALKEALAPVPIPFAAAQQRGPRKPIK
                     CWNCGKEGHSARQCRAPRRQGCWKCGKMDHVMAKCPDRQAGFLGLGPWGKKPRNFPMA
                     QVHQGLTPTAPPEDPAVDLLKNYMQLGKQQREKQRESREKPYKEVTEDLLHLNSLFGG
                     DQ"
     CDS             2229..5411
                     /function="protease, reverse transcriptase, endonuclease"
                     /product="pol polyprotein"
                     /gene="pol"
                     /codon_start=1
                     /translation="MLELWERRTLCKAMQSPKKTGMLEMWKNGPCYGQMPRQTGGFFR
                     PWSMGKEAPQFPHGSSASGADANCSPRGPSCGSAKELHAAGQAAERKAERKQREALQG
                     GDRGFAAPQFSLWRRPVVTAHIEGQPVEVLLDTGADDSIVTGIELGLHYTPKIVGGIG
                     GFINTKEYKNVEIEVLGKRIKGTIMTGDTPINIFGRNLLTALGMSLNLPIAKVEPVKV
                     ALKPGKDGPKLKQWPLSKEKIVALREICEKMEKDGQLEEAPPTNPYNTPTFAIKKKDK
                     NKWRMLIDFRELNRVTQDFTEVQLGIPHPAGLAKRKRITVLDIGDAYFSIPLDEEFRQ
                     YTAFTLPSVNNAEPGKRYIYKVLPQGWKGSPAIFQYTMRHVLEPFRKANPDVTLVQYM
                     DDILIASDRTDLEHDRVVLQLKELLNSIGFSTPEEKFQKDPPFQWMGYELWPTKWKLQ
                     KIELPQRETWTVNDIQKLVGVLNWAAQIYPGIKTKHLCRLIRGKMTLTEEVQWTEMAE
                     AEYEENKIILSQEQEGCYYQEGKPLEATVIKSQDNQWSYKIHQEDKILKVGKFAKIKN
                     THTNGVRLLAHVIQKIGKEAIVIWGQVPKFHLPVEKDVWEQWWTDYWQVTWIPEWDFI
                     STPPLVRLVFNLVKDPIKGEETYYTDGSCNKQSKEGKAGYITDRGKDKVKVLEQTTNQ
                     QAELEAFLMALTDSGPKANIIVDSQYVMGIITGCPTESESRLVNQIIEEMIKKSEIYV
                     AWVPAHKGIGGNQEIDHLVSQGIRQVLFLEKIEPAQEEHDKYHSNVKELVFKFGLPRI
                     VARQIVDTCDKCHQKGEAIHGQVNSDLGTWQMDCTHLEGKIIIVAVHVASGFIEAEVI
                     PQETGRQTALFLLKLAGRWPVTHLHTDNGANFASQEVKMVAWWAGIEHTFGVPYNPQS
                     QGVVEAMNHHLKNQIDRIREQANSVETIVLMAVHCMNFKRRGGIGDMTPAERLINMIT
                     TEQEIQFQQSKNSKFKNFRVYYREGRDQLWKGPGELLWKGEGAVILKVGTDIKVVPRR
                     KAKIIKDYGGGKEVDSSSHMEDTGEAREVA"
     repeat_region   2489..2522
                     /rpt_type=direct
                     /rpt_unit=2489..2500
                     /rpt_unit=2501..2512
                     /rpt_unit=2513..1522
     CDS             5341..5985
                     /function="virion infectivity factor"
                     /product="vif protein"
                     /gene="vif"
                     /codon_start=1
                     /translation="MEEGKRWIAVPTWRIPERLERWHSLIKYLKYKTKDLQKVCYVPH
                     YKVGWAWWTCSRVIFPLQEGSHLEVQGYWHLTPERGWLSTYAVRITWYSRNFWTDVTP
                     DYADILLHSTYFPCFTAGEVRRVIRGEQLLSCCKFPRAHRYQVPSLQYLALRVVSYVR
                     SQGENPTWKQWRRDSRRSLRMAKQNSRGDKQRGGKPPTKGANFPGLAKVLGILA"
     CDS             5813..6151
                     /product="vpx protein"
                     /gene="vpx"
                     /codon_start=1
                     /translation="MSDPRERIPPGNSGEETVGEAFEWLNRTVEEINREAVNHLPREL
                     IFQVWQRSWEYWHDEQGMSQSYVKYRYLCLIQKALFMHCKKGCRCLGEGHGAGGWRPG
                     PPPPPPPGLA"
     CDS             6152..6457
                     /product="vpr protein"
                     /gene="vpr"
                     /codon_start=1
                     /translation="MEERPPENEGPQREPWDEWVVEVLEELKEEALKHFDPRLLTALG
                     NHIYNRHGDTLEGAGELIRILQRALFMHFRGGCNHSRIGQPGGGNPLSTIPPSRGVL"
     CDS             6605..9241
                     /product="env polyprotein"
                     /gene="tat"
                     /codon_start=1
                     /translation="MGCLGNQLLIAILLLSVYGIYCTQYVTVFYGVPAWRNATIPLFC
                     ATENRDTWGTTQCLPDNGDYSELALNVTESFDAWENTVTEQAIEDVWQLFETSIKPCV
                     KLSPLCITMRCNKSETDKWGLTKSLTTTAPTAPTAASKIDMVNETSSCITHDNCTGLE
                     QEQMIGCKFNMTGLKRDKTKEYNETWYSTDLVCEQGNSTDNESRCYMNHCNTSIIQES
                     CDKHYWDTIRFRYCAPPGYALLRCNDTNYSGFMPKCSKVVVSSCTRMMETQTSTWFGF
                     NGTRAENRTYIYWHGRDNRTIISLNKYYNLTMKCRRPGNKTVLPVTIMSGLVFHSQPI
                     NDRPKQAWCWFGGNWKDAIKEMKQTIVKHPRYTGTNNTDKINLTAPRGGDPEVTFMWT
                     NCRGEFLYCKMNWFLNWVEDRDVTNQRPKERHRRNYVPCHIRQIINTWHKVGKNVYLP
                     PREGDLTCNSTVTSLIANIDWTDGNQTNITMSAEVAELYRLELGDYKLVEITPIGLAP
                     TDVKRYTTGGTSRNKRGVFVLGFLGFLATAGSAMGAASLTLTAQSRTLLAGIVQQQQQ
                     LLDVVKRQQELLRLTVWGTKNLQTRVTAIEKYLKDQAQLNAWGCAFRQVCHTTVPWPN
                     ASLTPDWNNDTWQEWERKVDFLEENITALLEEAQIQQEKNMYELQKLNSWDVFGNWFD
                     LASWIKYIQYGVYIVVGVILLRIVIYIVQMLAKLRQGYRPVFSSPPSYFQQTHIQQDP
                     ALPTREGKEGDGGEGGGNSSWPWQIEYIHFLIRQLIRLLTWLFSNCRTLLSRAYQILQ
                     PILQRLSAALQRIREVLRTELTYLQYGWSYFQEAVQVGWRSATETLAGAWGDLWETLR
                     RGGRWILAIPRRIRQGLELTLL"
     exon            8801..>8900
                     /number=2
                     /product="tat protein"
     exon            8801..>9057
                     /number=2
                     /product="rev protein"
     exon            8804..>8900
                     /number=2
                     /note="alternate second exon"
                     /product="tat protein"
     exon            8804..>9057
                     /number=2
                     /note="alternate second exon"
                     /product="rev protein"
     CDS             9075..9866
                     /function="negative regulatory factor"
                     /product="nef protein"
                     /gene="nef"
                     /codon_start=1
                     /translation="MGGAISRRRSKSAGDLRQRLLRARGETYGRLLGEVEDGYSQSLG
                     GLDKGLSSLSCEGQKYNQGQYMNTPWRNPAEEREKLAYRKQNMDDVDEEDDDLVGVPV
                     MPRVPLRTMSYKLAVDMSHFIKEKGGLEGIYYSARRHRILDMYLEKEEGIIPDWQDYT
                     SGPGIRYPKTFGWLWKLVPVNVSDEAQEDERHYLMQPAQTSKWDDPWGEVLAWKFDPT
                     LAYTYEAYVRYPEEFGSKSGLPEEEVRRRLTARGLLNMADKKETR"
     LTR             9460..10277
                     /note="3'LTR"
     LTR             9460..9977
                     /note="3'LTR U3 region"
     variation       9501..9512
                     /note="pC8"
     variation       9646
                     /note="replace(9646,'a')"
     variation       9815
                     /note="replace(9815,'a')"
     variation       9819
                     /note="replace(9819,'g')"
     variation       9931
                     /note="replace(9931,'g')"
     repeat_unit     9978..10153
                     /rpt_type=terminal
     variation       10061
                     /note="replace(10061,'g')"
     variation       10122
                     /note="replace(10122, 'c')"
     polyA_signal    10130..10135
     LTR             10154..10277
                     /note="3'LTR U5 region"
BASE COUNT     3457 a   1938 c   2579 g   2303 t
ORIGIN
        1 tggaagggat ttattacagt gcaagaagac atagaatctt agacatgtac ttagaaaagg
       61 aggaaggcat cataccagat tggcaggatt acacctcagg accaggaatt agatacccaa
      121 agacatttgg ctggctatgg aaattagtcc ccgtaaatgt atcagatgag gcacaggagg
      181 atgagaggca ttatttaatg cagccagctc aaacttccaa gtgggatgac ccttggggag
      241 aggttctagc atggaagttt gatccaactc tagcctacac ttatgaggca tatgttagat
      301 acccagaaga gtttggaagc aagtcaggcc tgccagagga agaggttaga agaaggctaa
      361 ccgcaagagg ccttcttaac atggctgaca agaaggaaac tcgctgagac agcagggact
      421 ttccacaagg ggatgttacg gggaggtact ggggaggagc cggtcgggaa cacccacttt
      481 cttgatgtat aaatatcact gcatttcgct ctgtattcag tcgctctgcg gagaggctgg
      541 cagattgagc cctgggaggt tctctccagc actagcaggt agagcctggg tgttccctgc
      601 tagactctca ccagcacttg gccggtgctg ggcagagtgg ctccacgctt gcttgcttaa
      661 agacctcttc aataaagctg ccattttaga agtaagctag tgtgtgttcc catctctcct
      721 agtcgccgcc tggtcaactc ggtactcggt aataaggaga ccctggtctg ttaggaccct
      781 ttctgctttg agaaaccgaa gcaggaaaat ccctagcaga ttggcgcctg aacagggact
      841 tgaaggagag tgagagactc ctgagtacgg ctgagtgaag gcagtaaggg cggcaggaac
      901 caaccacgac ggagtgctcc tataaaggcg cgggtcggta ccagacggcg tgaggagcgg
      961 gagaggaaga ggcctccggt tgcaggtaag tgcaacacaa aaaaagaaat agctgtcttt
     1021 tatccaggaa gggataataa gatagagtgg gagatgggcg cgagaaactc cgtcttgtca
     1081 gggaagaaag cagatgaatt agaaaaaatt aggctacgac ccggcggaaa gaaaaagtac
     1141 atgttgaagc atgtagtatg ggcagcaaat gaattagata gatttggatt agcagaaagc
     1201 ctgttggaga acaaagaagg atgtcaaaaa atactttcgg tcttagctcc attagtgcca
     1261 acaggctcag aaaatttaaa aagcctttat aatactgtct gcgtcatctg gtgcattcac
     1321 gcagaagaga aagtgaaaca cactgaggaa gcaaaacaga tagtgcagag acacctagtg
     1381 gtggaaacag gaacagcaga aactatgcca aaaacaagta gaccaacagc accatctagc
     1441 ggcagaggag gaaattaccc agtacaacaa ataggtggta actatgtcca cctgccatta
     1501 agcccgagaa cattaaatgc ctgggtaaaa ttgatagagg aaaagaaatt tggagcagaa
     1561 gtagtgccag gatttcaagc actgtcagaa ggctgcaccc cctatgacat taatcagatg
     1621 ttaaattgtg tgggagacca tcaagcggct atgcagatta tcagagacat tataaatgag
     1681 gaggctgcag attgggactt gcagcaccca caaccagctc cacaacaagg acagcttagg
     1741 gagccgtcag gatcagatat tgcaggaaca actagttcag tagatgaaca aatccagtgg
     1801 atgtacagac aacagaaccc cataccagta ggcaacattt acaggagatg gatccaactg
     1861 gggttgcaaa aatgtgtcag aatgtataac ccaacaaaca ttctagatgt aaaacaagga
     1921 ccaaaagagc catttcagag ctatgtagac aggttctaca aaagcttaag agcagaacaa
     1981 acagatgcag cagtaaagaa ttggatgact caaacactgc tgattcaaaa tgctaaccca
     2041 gattgcaagt tagtactgaa gggactgggt gtgaatccca ccctagaaga aatgctgacg
     2101 gcttgtcaag gagtaggggg accaggacag aaggctagat taatggcaga agccctgaaa
     2161 gaggccctcg caccagtgcc aatccctttt gcagcagccc aacagagggg accaagaaag
     2221 ccaattaagt gttggaattg tgggaaagaa ggacactctg caaggcaatg cagagcccca
     2281 agaagacagg gatgctggaa atgtggaaaa atggaccatg ttatggccaa atgcccagac
     2341 agacaggcgg gttttttagg ccttggtcca tggggaaaga agccccgcaa tttccccatg
     2401 gctcaagtgc atcaggggct gacgccaact gctcccccag aggacccagc tgtggatctg
     2461 ctaaagaact acatgcagct gggcaagcag cagagagaaa agcagagaga aagcagagag
     2521 aagccttaca aggaggtgac agaggatttg ctgcacctca attctctctt tggaggagac
     2581 cagtagtcac tgctcatatt gaaggacagc ctgtagaagt attactagat acaggggctg
     2641 atgattctat tgtaacagga atagagttag gtctacatta taccccaaaa atagtaggag
     2701 gaataggagg ttttattaat actaaagaat acaaaaatgt agaaatagaa gttttaggca
     2761 aaaggattaa agggacaatc atgacagggg acaccccgat taacattttt ggtagaaatt
     2821 tactaacagc tctggggatg tctttaaacc ttcccatagc taaagtagag cctgtaaaag
     2881 tcgccttaaa gccaggaaag gatggaccaa aattgaagca gtggccatta tcaaaagaaa
     2941 agatagttgc attaagagaa atctgtgaaa agatggaaaa ggatggtcag ttggaggaag
     3001 ctcccccgac caatccatac aacaccccca catttgctat aaagaaaaaa gataagaaca
     3061 aatggagaat gctgatagat tttagggaac taaatagggt cactcaggac tttacagaag
     3121 tccaattagg aataccacac cctgcaggac tagcaaaaag gaaaaggatt acagtactgg
     3181 atataggtga tgcatatttc tccatacctc tagatgaaga atttaggcag tacactgcct
     3241 ttactttacc atcagtaaat aatgcagagc caggaaagcg atacatttat aaggttctgc
     3301 ctcagggatg gaaagggtca ccagccatct tccaatacac tatgagacat gtgctagaac
     3361 ccttcaggaa ggcaaatcca gatgtgacct tagtccagta tatggatgac atcttaatag
     3421 ctagtgacag gacagacctg gaacatgaca gggtagtttt acagttaaag gaactcttga
     3481 atagcatagg gttttctacc ccagaagaaa aattccaaaa agatccccca tttcaatgga
     3541 tggggtacga attgtggcca acaaaatgga agttgcaaaa gatagagttg ccacaaagag
     3601 agacctggac agtgaatgat atacagaagt tagtaggagt attaaattgg gcagctcaaa
     3661 tttatccagg tataaaaacc aaacatctct gtaggttaat tagaggaaaa atgactttaa
     3721 cagaggaagt tcagtggact gagatggcag aagcagaata tgaggaaaat aaaataattc
     3781 tcagtcagga acaagaagga tgttattacc aagagggcaa gccattagaa gccacggtaa
     3841 taaagagtca ggacaatcag tggtcttata aaattcacca agaagacaaa atactgaaag
     3901 taggaaagtt tgcaaagata aagaatacac ataccaatgg agttagacta ttagcacatg
     3961 taatacagaa aataggaaag gaagcaatag tgatctgggg acaggttcca aaattccact
     4021 taccagttga gaaggatgta tgggaacagt ggtggacaga ctattggcag gtaacctgga
     4081 taccagaatg ggattttatc tcaacaccac cactagtaag attagtcttc aatctagtga
     4141 aggaccctat aaagggagaa gaaacctatt atacagatgg atcatgtaat aaacagtcaa
     4201 aagaagggaa agcaggatat atcacagata ggggcaaaga caaagtaaaa gtgttagaac
     4261 agactactaa tcaacaagca gaattggaag catttctcat ggcattgaca gactcagggc
     4321 caaaggcaaa tattatagta gattcacaat atgttatggg aataataaca ggatgcccta
     4381 cagaatcaga gagcaggcta gttaaccaaa taatagaaga aatgattaaa aagtcagaaa
     4441 tttatgtagc atgggtacca gcacacaaag gtataggagg aaaccaagaa atagaccacc
     4501 tagttagtca agggattaga caagttctct tcttggaaaa gatagagcca gcacaagagg
     4561 aacatgataa gtaccatagt aatgtaaaag aattggtatt caaatttgga ttacccagaa
     4621 tagtggccag acagatagta gacacatgtg ataaatgtca tcaaaaagga gaagctatac
     4681 atgggcaggt aaattcagat ctagggactt ggcaaatgga ttgtacccat ctagagggaa
     4741 aaataatcat agttgcagta catgtagcta gtggattcat agaagcagaa gtaattccac
     4801 aagagacagg aagacagaca gcactatttc tgttaaaatt ggcaggcaga tggcctgtta
     4861 cacatctaca cacagataat ggtgctaact ttgcctcgca agaagtaaag atggttgcat
     4921 ggtgggcagg gatagagcac acctttgggg taccatacaa tccacagagt cagggagtag
     4981 tggaagcaat gaatcaccac ctgaaaaatc aaatagatag aatcagggaa caagcaaatt
     5041 cagtagaaac catagtatta atggcagttc attgcatgaa ttttaaaaga aggggaggaa
     5101 taggggatat gactccagca gaaagattaa ttaacatgat cactacagaa caagaaatac
     5161 aatttcaaca atcaaaaaac tcaaaattta aaaattttcg ggtctattac agagaaggca
     5221 gagatcaact gtggaaggga cccggtgagc tattgtggaa aggggaagga gcagtcatct
     5281 taaaggtagg gacagacatt aaggtagtac ccagaagaaa ggctaaaatt atcaaagatt
     5341 atggaggagg gaaagaggtg gatagcagtt cccacatgga ggataccgga gaggctagag
     5401 aggtggcata gcctcataaa atatctgaaa tataaaacta aagatctaca aaaggtttgc
     5461 tatgtgcccc attataaggt cggatgggca tggtggacct gcagcagagt aatcttccca
     5521 ctacaggaag gaagccattt agaagtacaa gggtattggc atttgacacc agaaagaggg
     5581 tggctcagta cttatgcagt taggataacc tggtactcaa ggaacttttg gacagatgta
     5641 acaccagact atgcagacat tttactgcat agcacttatt tcccttgctt tacagcggga
     5701 gaagtgagaa gggtcatcag gggagaacaa ctgctgtcct gctgcaagtt cccgagagct
     5761 cataggtacc aggtaccaag tctacagtac ttagcactga gagtagtaag ttatgtcaga
     5821 tcccagggag agaatcccac ctggaaacag tggagaagag acagtaggag aagccttcga
     5881 atggctaaac agaacagtag aggagataaa cagagaggcg gtaaaccacc taccaaggga
     5941 gctaattttc caggtttggc aaaggtcttg ggaatactgg catgatgaac aagggatgtc
     6001 acaaagctat gtaaaataca gatacttgtg tttaatacaa aaggctttat ttatgcattg
     6061 caagaaaggc tgtagatgtc taggggaagg acacggggca gggggatgga gaccaggacc
     6121 tcctcctcct ccccctccag gactagcata aatggaagaa agacctccag aaaatgaagg
     6181 cccacaaagg gaaccatggg atgaatgggt agtggaggtt ctggaagaac tgaaagaaga
     6241 agctttaaaa cattttgatc ctcgcttgct aactgcactt ggtaatcata tctataatag
     6301 acatggagac acccttgagg gagcaggaga actcattaga atcctccaac gagcgctctt
     6361 catgcatttc agaggcggat gcaaccactc cagaatcggc caacctgggg gaggaaatcc
     6421 tctctcaact ataccgccct ctagaggcgt gctataacac atgctattgt aaaaagtgtt
     6481 gctaccattg ccagttttgt tttcttaaaa agggcttggg gatatgttat gagcagtcac
     6541 gaaagagaag aagaactccg aaaaaggcta aggctaatac atcttctgca tcaaacaagt
     6601 aagtatggga tgtcttggga atcagctgct tatcgccatc ttgcttttaa gtgtctacgg
     6661 gatctattgt actcaatatg tcacagtctt ttatggtgta ccagcttgga ggaatgcgac
     6721 aattcccctc ttctgtgcaa ccgagaatag ggatacttgg ggaacaactc agtgcctacc
     6781 agataatggt gattattcag aattggccct taacgttaca gaaagctttg atgcctggga
     6841 gaatacagtc acagaacagg caatagagga tgtatggcaa ctctttgaga cctcaataaa
     6901 gccttgtgta aaattatccc cattatgcat tactatgaga tgcaataaaa gtgagacaga
     6961 taaatgggga ttgacaaaat cattaacaac aacagcacca acagcaccaa cggcagcatc
     7021 aaaaatagac atggtcaatg agactagttc ttgtataact catgataatt gcacaggctt
     7081 ggaacaagag caaatgatag gctgtaaatt caacatgaca gggttaaaaa gagacaagac
     7141 aaaggagtac aatgaaactt ggtactctac agatttggtt tgtgaacaag ggaatagcac
     7201 tgataatgaa agtagatgct acatgaatca ctgtaacact tctattatcc aagagtcttg
     7261 tgacaagcat tattgggata ctattagatt taggtattgt gcacctccag gttatgcttt
     7321 gcttagatgt aatgacacaa attattcagg ctttatgcct aaatgttcta aggtggtggt
     7381 ctcttcatgc acaaggatga tggagacaca gacttctact tggtttggct ttaatggaac
     7441 tagagcagaa aatagaactt atatttactg gcatggtagg gataatagga ctataattag
     7501 tttaaataag tattataatc taacaatgaa atgtagaaga ccaggaaata agacagtttt
     7561 accagtcacc attatgtctg gattggtttt ccactcacaa ccaatcaatg ataggccaaa
     7621 gcaggcatgg tgttggtttg gaggaaattg gaaggatgca ataaaagaga tgaagcagac
     7681 cattgtcaaa catcccaggt atactggaac taacaatact gataaaatca atttgacggc
     7741 tcctagagga ggagatccgg aagttacctt catgtggaca aattgcagag gagagttcct
     7801 ctactgtaaa atgaattggt ttctaaattg ggtagaagat agggatgtaa ctaaccagag
     7861 gccaaaggaa cggcatagaa ggaattacgt gccatgtcat attagacaaa taatcaacac
     7921 ttggcataaa gtaggcaaaa atgtttattt gcctccaaga gagggagacc tcacgtgtaa
     7981 ctccacagtg accagtctca tagcaaacat agattggact gatggaaacc aaactaatat
     8041 caccatgagt gcagaggtgg cagaactgta tcgattggaa ttgggagatt ataaattagt
     8101 agagatcact ccaattggct tggcccccac agatgtgaag aggtacacta ctggtggcac
     8161 ctcaagaaat aaaagagggg tctttgtgct agggttcttg ggttttctcg caacggcagg
     8221 ttctgcaatg ggcgcggcgt cgttgacgct gaccgctcaa tcccggactt tattggctgg
     8281 gatagtgcag caacagcaac agctgttgga cgtggtcaag agacaacaag aattgttgcg
     8341 actgaccgtc tggggaacaa agaacctcca gactagggtc actgccatcg agaagtactt
     8401 aaaggaccag gcgcagctga atgcttgggg atgtgcgttt agacaagtct gccacactac
     8461 tgtaccatgg ccaaatgcaa gtctaacacc agactggaac aatgatactt ggcaagagtg
     8521 ggagcgaaag gttgacttct tggaggaaaa tataacagcc ctcctagaag aggcacaaat
     8581 tcaacaagag aagaacatgt atgaattaca aaagttgaat agctgggatg tgtttggcaa
     8641 ttggtttgac cttgcttctt ggataaagta tatacaatat ggagtttata tagttgtagg
     8701 agtaatactg ttaagaatag tgatctatat agtacaaatg ctagctaagt taagacaggg
     8761 gtataggcca gtgttctctt ccccaccctc ttatttccag cagacccata tccaacagga
     8821 cccggcactg ccaaccagag aaggcaaaga aggagacggt ggagaaggcg gtggcaacag
     8881 ctcctggcct tggcagatag aatatattca tttcctgatc cgccaactga tacgcctctt
     8941 gacttggcta ttcagcaact gcagaacctt gctatcgaga gcataccaga tcctccaacc
     9001 aatactccag aggctctctg cggccctaca gagaattcga gaagtcctca ggactgaact
     9061 gacctaccta caatatgggt ggagctattt ccaggaggcg gtccaagtcg gctggagatc
     9121 tgcgacagag actcttgcgg gcgcgtgggg agacttatgg gagactctta ggagaggtgg
     9181 aagatggata ctcgcaatcc ctaggaggat tagacaaggg cttgagctca ctctcttgtg
     9241 agggacagaa atacaatcag gggcagtata tgaatactcc atggagaaac ccagctgaag
     9301 agagagaaaa attagcatac agaaaacaaa atatggatga tgtagatgag gaagatgatg
     9361 acttggtagg ggtaccagtg atgccacgag ttcccctaag aacaatgagt tacaaattgg
     9421 cagtagatat gtctcatttt ataaaagaaa aggggggact ggaagggatt tattacagtg
     9481 caagaagaca tagaatctta gacatgtact tagaaaagga ggaaggcatc ataccagatt
     9541 ggcaggatta cacctcagga ccaggaatta gatacccaaa gacatttggc tggctatgga
     9601 aattagtccc cgtaaatgta tcagatgagg cacaggagga tgagaggcat tatttaatgc
     9661 agccagctca aacttccaag tgggatgacc cttggggaga ggttctagca tggaagtttg
     9721 atccaactct agcctacact tatgaggcat atgttagata cccagaagag tttggaagca
     9781 agtcaggcct gccagaggaa gaggttagaa gaaggctaac cgcaagaggc cttcttaaca
     9841 tggctgacaa gaaggaaact cgctgagaca gcagggactt tccacaaggg gatgttacgg
     9901 ggaggtactg gggaggagcc ggtcgggaac acccactttc ttgatgtata aatatcactg
     9961 catttcgctc tgtattcagt cgctctgcgg agaggctggc agattgagcc ctgggaggtt
    10021 ctctccagca ctagcaggta gagcctgggt gttccctgct agactctcac cagcacttgg
    10081 ccggtgctgg gcagagtggc tccacgcttg cttgcttaaa gacctcttca ataaagctgc
    10141 cattttagaa gtaagctagt gtgtgttccc atctctccta gtcgccgcct ggtcaactcg
    10201 gtactcggta ataagaagac cctggtctgt taggaccctt tctgctttgg gaaaccgaag
    10261 caggaaaatc cctagca
//