From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Pseudomonas sp. alcohol dehydrogenase (terpD) gene, complete cds;
Date: 24 Jul 92 10:09:12 GMT
Distribution: bionet
Checksum: 17106    14

LOCUS       PSETERP      6620 bp ds-DNA             BCT       24-JUL-1992
DEFINITION  Pseudomonas sp. alcohol dehydrogenase (terpD) gene, complete cds;
            aldehyde dehydrogenase (terpE) gene, complete cds; P450terp (terpC)
            gene, complete cds; terpredoxin reductase (terpA) gene, complete
            cds; terpredoxin (terpB) gene, complete cds.
ACCESSION   M91440
KEYWORDS    P450terp; alcohol dehydrogenase; aldehyde dehydrogenase;
            terpA gene; terpB gene; terpC gene; terpD gene; terpE gene;
            terpredoxin; terpredoxin reductase.
SOURCE      Pseudomonas sp. DNA.
  ORGANISM  Pseudomonas sp.
            Prokaryotae; Gracilicutes; Scotobacteria; Pseudomonadaceae.
REFERENCE   1  (bases 1 to 6620)
  AUTHORS   Peterson,J.A., Lu,J.-Y., Geisselsoder,J., Graham-Lorence,S.,
            Carmona,C., Witney,F. and Lorence,M.C.
  TITLE     Cytochrome P-450-terp: Isolation and purification of the protein
            and cloning and sequencing of tis operon
  JOURNAL   J. Biol. Chem. 267, 14193-14203 (1992)
  STANDARD  full staff_entry
FEATURES             Location/Qualifiers
     terminator      575..621
                     /note="putative terminator"
                     /note="putative"
     RBS             736..740
                     /standard_name="Shine-Dalgarno"
                     /gene="terpD"
     CDS             752..1711
                     /product="alcohol dehydrogenase"
                     /gene="terpD"
                     /codon_start=1
                     /translation="MPRREMMKCLFVSESCVCHTDLAIKDGVLPFPLPAVLGHEGSGI
                     VEAVGPGVKHLKPGDAVVMTFASCGHCASCEHERPSYCLDFGAQNYSAQRADGPVLLS
                     QGDEVISGFFFGQSSFSSMAMAREHNLVKIDALVDDAPIELLGPLGCGVQTGAGAVMI
                     SLDVRAGRSFLVLGGGAVGLSAVMAAKLRGCSRIIVSEPSAAKREQALALGATEVIDP
                     LNENLVERVQQITEGQGCDYALECTGLVSVMEQAIDSMAMRGQLAVVGVPPKLDATAA
                     VSPLALIQKGLKLMGVIEGDSCRVYSSTSCTRSSRLGVFRSPR"
     CDS             1754..3220
                     /product="aldehyde dehydrogenase"
                     /gene="terpE"
                     /codon_start=1
                     /translation="MSSRESASRRPDPLRVTIMQSPSAFSLTIGGHGVATSDYLPVLN
                     PATRQPIGQCPVASEEHVEQARAAAGDAFAVWRQVPYGKRCEQLQALAGILEAHLDEL
                     AQLLTHEQGKPLADARGELAGAIAFCQCAGSLPLPEVVHEQSATRLSKTVREPLGVVV
                     AIVPWNYPVSIAFVKPDRRVGCRKHADSQAGADHAADYLRIGELIRDALPAGVINVLA
                     GNDDLGPWLTGHADVAKISFTGSSVTGRHVARSAAADLKRLTLELGGNDAAVVMADSL
                     SDAVVERVFWSAFTNAGQICMATKRLYIHESIYEAFRDKLVAYAQNVVIGDGSQPGVT
                     MGPLQNAKQFAKVMSLIDAVRQRGGRLIECGQMRGGDGYFLPITFVDLPDESAPEVVE
                     EAFGPLLPLLKFRDVDEVIERVNAARTGWLAASGVVIGRSAHGLQQPSTRSVWSTTGC
                     DHAVYSFGGMKASGYGAESGLEGLLEFTTQKTVIIQQQ"
     RBS             3221..3225
                     /standard_name="Shine-Dalgarno"
                     /gene="terpC"
     CDS             3232..4518
                     /product="P450terp"
                     /gene="terpC"
                     /codon_start=1
                     /translation="MDARATIPEHIARTVILPQGYADDEVIYPAFKWLRDEQPLAMAH
                     IEGYDPMWIATKHADVMQIGKQPGLFSNAEGSEILYDQNNEAFMRSISGGCPHVIDSL
                     TSMDPPTHTAYRGLTLNWFQPASIRKLEENIRRIAQASVQRLLDFDGECDFMTDCALY
                     YPLHVVMTALGVPEDDEPLMLKLTQDFFGVHEPDEQAVAAPRQSADEAARRFHETIAT
                     FYDYFNGFTVDRRSCPKDDVMSLLANSKLDGNYIDDKYINAYYVAIATAGHDTTSSSS
                     GGAIIGLSRNPEQLALAKSDPALIPRLVDEAVRWTAPVKSFMRTALADTEVRGQNIKR
                     GDRIMLSYPSANRDEEVFSNPDEFDITRFPNRHLGFGWGAHMCLGQHLAKLEMKIFFE
                     ELLPKLKSVELSGPPRLVATNFVGGPKNVPIRFTKA"
     RBS             4517..4521
                     /standard_name="Shine-Dalgarno"
                     /gene="terpA"
     CDS             4529..5758
                     /product="terpredoxin reductase"
                     /gene="terpA"
                     /codon_start=1
                     /translation="MGERRDTTVIVGAGHAGTAAAFFLREFGYHGRVLLLSAETQHPY
                     QRPPLSKEYLLAQHSTPSLLKGKDSYARADIELCLQDDVLSITPASRQVKSSQGSYTY
                     DHLILATGSHPRFMATLGQADNLCYLSDWDDAGRIRQQLGEASRIVVLGGGFIGLEIA
                     SSACKMGKHVTVIERAPRLLSRVVSEAFATFIGDIHLGNGIELRLGEEVREVRRCTSG
                     VRVDAVFLSDGQLLECDMLVIGVGSEPRMELATAAGLACASGVLVDEHCHTSDPFISA
                     IGDCVAVCPSPGHQLPRRESVQNATEQARLVAARLSGRPVPPVQTPWFWSDQLQARIN
                     LAGERPAQGQVIVRRYGGDKVSMLYLQDQQLVAIEACNMPGDCLLARRAIGQNHSLDL
                     ARLVDADVPLKDALHFA"
     RBS             5759..5761
                     /standard_name="Shine-Dalgarno"
                     /gene="terpB"
     CDS             5770..6090
                     /product="terpredoxin"
                     /gene="terpB"
                     /evidence=EXPERIMENTAL
                     /codon_start=1
                     /translation="MPRVVFIDEQSGEYAVDAQDGQSLMEVATQNGVPGIVAECGGSC
                     VCATCRIEIEDAWVEIVGEANPDENDLLQSTGEPMTAGTRLSCQVFIDPSMDGLIVRV
                     PLPA"
     terminator      6109..6147
BASE COUNT     1342 a   1841 c   2021 g   1416 t
ORIGIN
        1 atcctctaga gtcgacctgc agcacttcgg cgtcgacgtt gtcaccgacg aaggtgcgca
       61 ggtcgcgacg gtagcggttg gtgaccaggt agtcctcgac gatcagctcc ctgtccacgc
      121 ccagcatcag aagaatcact gcggcagcga aaccggttcg atctttgccg ctggtacagt
      181 gaatcagcgc gggcagggca tcgggttcga gcagatgacg aatcagttgg ccataacgct
      241 cggcatgctc cagcggcaag cgccggtaca tttccagaaa gatcgcctgc gcgccttcca
      301 ctgttagctg tcgatcacgc acgccacgaa tcacttcgct ggcaccgtgt ggaatgaagc
      361 cgagcaagcg agtgatcggt agcggtccat cacccaagcg gttgggcagg gcgagacgct
      421 catgctcgtg gcgcaggtcg tagatgcctc gcaggttcag cgcggcgaag gtcagcacgt
      481 cttcggctgt gagctgggcc aggaagtcgg agcggaacaa caggccgtgc gaacccgtcg
      541 accttcatga gcgggataac cgcccaagtc gcggcaacgg ataaaaatgc gccaagccat
      601 gcttgacgta tttttatttt agattagtat tcatcagtac taaatttccc tgtctttcca
      661 acaacaacaa agagactagc cgaaatggaa gtgaccgccg ctgtcgcgcg acgcggggcc
      721 gataagttct cgattgagag cttgtcaatg gatgccacgc cgcgaaatga tgaagtgctt
      781 gttcgtgtca gagagttgcg tctgccatac ggatctcgca attaaggatg gtgtgttacc
      841 atttcccttg cctgccgttc tcggccatga aggcagcggc atcgtcgaag cggttgggcc
      901 tggtgtaaag cacttgaaac ccggtgatgc tgtggtgatg acctttgcat cctgcggcca
      961 ctgcgccagt tgtgagcacg aacggccgtc ctattgcctg gatttcggtg cgcagaacta
     1021 ctcggctcaa cgtgctgatg gtccggtcct gcttagccag ggtgatgaag ttatctcggg
     1081 tttctttttc ggtcagtcat cgttctccag catggcgatg gcccgtgaac acaatttggt
     1141 gaagattgac gcactggtcg atgacgcacc catcgaactg ctcggaccgc ttgggtgcgg
     1201 agtgcagact ggggccgggg ctgtgatgat ctccctcgac gtgcgggctg gccgcagttt
     1261 tctggtgctc ggcggcggcg ccgtcggtct gagtgcagtg atggccgcca agctgcgtgg
     1321 ttgctcacgc atcatcgttt ccgagccttc ggctgccaag cgtgagcagg cgctggcctt
     1381 gggcgcaacc gaggtaattg acccgttgaa cgagaacctg gtcgagcgcg tgcagcagat
     1441 caccgaaggg cagggctgcg attacgcgct ggagtgcacc ggtctggttt ccgtcatgga
     1501 gcaggcaatc gattccatgg ccatgcgcgg tcagttggcc gtggttggtg tgccacccaa
     1561 actcgatgca accgctgctg tcagccccct ggcgctgatc cagaaagggt tgaagctgat
     1621 gggtgtaatc gaaggcgaca gctgccgcgt gtattcatca acgagctgta cgcgcagttc
     1681 aaggctgggc gttttccgtt caccaagatg atccacacct atccgctgac atcgatcaat
     1741 caggccatcg ccgatgtcga gtcgggagtc tgcatcaagg cgtcctgatc ccctgagggt
     1801 tacaatcatg caaagtccat ccgcgttctc gctgacaatc ggcgggcatg gtgtcgctac
     1861 atccgattat ttgccagtgc tcaatccggc gacccgccaa cccatcgggc aatgccccgt
     1921 agcgagcgaa gagcacgtcg agcaggcgcg tgctgccgct ggtgacgctt ttgctgtctg
     1981 gcgtcaggtg ccttatggca aacgctgcga gcaactgcaa gcactggccg gaattctcga
     2041 agcccatttg gacgaactgg cccaactgct gacccacgag cagggcaagc cgctagccga
     2101 tgcgcgaggc gagctggcgg gtgcaattgc cttctgccaa tgcgcgggca gcctgccgct
     2161 gccagaggtg gtgcacgaac aaagtgccac gcggctgtcc aaaaccgtgc gcgaacccct
     2221 cggcgttgta gtggcgattg tgccctggaa ctacccggtc tcgattgcct tcgtcaaacc
     2281 tgaccgccgc gttggctgcc ggaaacacgc tgattctcaa gccggcgccg accacgccgc
     2341 tgactacctg cgcatcggcg aactgatacg tgatgcgctg ccggccgggg taatcaatgt
     2401 attggcaggc aacgatgacc tgggcccctg gctcaccggg catgccgatg tggccaagat
     2461 cagcttcact ggttcgagtg ttacagggcg ccatgtggcc cgttcagcag cagccgacct
     2521 caagcgcctg acccttgaac tgggtggcaa cgatgctgcc gtggtgatgg ccgacagtct
     2581 tagcgatgcg gtggttgaga gggtgttttg gtctgccttc accaacgccg gacagatctg
     2641 catggccacc aaacgcttgt atatccacga aagcatctac gaggcgtttc gcgacaagct
     2701 ggttgcctac gcacagaacg tggtgatcgg cgatggctcc cagccagggg tgaccatggg
     2761 accgctgcaa aacgccaagc agttcgccaa ggtcatgtcg ctgatcgacg ctgtgcgcca
     2821 gcgtggcggg cgtttgatcg agtgcgggca aatgcgtgga ggcgatggct atttcctacc
     2881 gatcaccttt gtcgacctgc cggacgagag cgccccagag gttgtcgagg aggctttcgg
     2941 gccgttgctg ccgctgctga agttcaggga tgtcgatgag gtcatcgaac gggtcaacgc
     3001 agcccgtacg ggctggctgg cagcgtctgg agtcgtgata gggaggtcgg cgcacgggtt
     3061 gcagcagcca tcgacacgca gcgtttggtc aacgactggc tgcgatcacg ccgtctactc
     3121 cttcggcggc atgaaggcgt cgggctatgg cgctgagagc gggctggaag ggcttctgga
     3181 attcaccacg caaaagaccg tgatcatcca acaacaataa gagaggcaaa catggacgct
     3241 agagccacca ttccggaaca tatcgcgcgt acggtaatcc tgcctcaggg gtatgccgat
     3301 gatgaggtca tctacccggc tttcaaatgg ttgcgtgatg agcaaccgct cgcgatggcc
     3361 catatcgaag gttacgaccc catgtggatc gcgaccaagc atgccgacgt catgcagatt
     3421 ggcaagcaac cgggactatt cagcaatgcc gaaggcagcg agattctcta cgatcagaac
     3481 aacgaagcct tcatgcgctc gatcagcggg gggtgccccc atgtaatcga ttcgttgacc
     3541 tcgatggacc cgcctacgca cacggcttat cgcggcttga ccctgaactg gttccaaccc
     3601 gccagcatcc gcaagctcga agagaatatt cgccggatcg cgcaagcctc ggtgcagcgt
     3661 ctgctcgatt ttgacggcga atgtgatttc atgacggatt gtgcgctgta ctacccgctg
     3721 cacgtggtga tgacggcgct gggggtgcct gaggatgacg agccgctcat gctcaagctg
     3781 acccaggatt tcttcggggt acacgaaccg gacgagcagg ccgttgctgc accgcggcag
     3841 tcggcggatg aagccgccag gcgcttccac gaaaccatcg ccactttcta cgactatttc
     3901 aatggtttca ccgtcgaccg ccgcagttgc cccaaggatg acgtgatgtc gctgctggcc
     3961 aacagcaagc ttgacggcaa ctacatcgat gacaagtaca tcaacgccta ctacgtggca
     4021 attgccactg cgggtcacga taccacctct tcatcgtccg gcggggctat catcggccta
     4081 tcgcgcaacc ctgagcaatt ggcgttggcc aaatccgatc ctgctctgat tccgcgtctg
     4141 gtcgatgaag cggtacgttg gaccgctccg gtcaagtcgt tcatgcggac cgcattggct
     4201 gacaccgaag tgcgcggcca gaacatcaag cgcggcgacc gcatcatgct ttcgtatcca
     4261 tcggccaatc gtgatgagga agtgtttagc aatcctgatg aattcgacat cacccgtttt
     4321 cccaatcgac acctgggttt tggctggggg gcacatatgt gcctcggcca gcatctagcc
     4381 aagttggaaa tgaaaatctt cttcgaggaa ctgttgccca agctcaagag cgttgaactg
     4441 tctgggccgc cgcggctggt ggcgacaaac ttcgtcggtg gcccgaaaaa cgtgccgatt
     4501 cgcttcacca aggcttagga gcactgtcat gggggaacgt cgagacacta cggtcatcgt
     4561 tggcgctggc cacgcaggaa cagccgccgc cttctttctg cgagagtttg gctatcacgg
     4621 cagggtgctt ttattgagcg cggagacgca gcacccttat cagcgcccac cgttatccaa
     4681 ggaatatttg cttgctcagc acagtacgcc gagcctgctg aagggcaagg actcctatgc
     4741 tcgtgccgat atcgagctgt gcctgcaaga tgatgttctt tcgataacgc cggcttcgcg
     4801 tcaggtgaaa agctcgcaag gtagttatac ctatgaccac ttgatccttg ccacaggctc
     4861 gcatccacgc ttcatggcta cgttaggcca ggccgataat ctctgttact tgtcggattg
     4921 ggatgatgcc ggacgtattc ggcagcagct cggtgaggcc agtcggatcg tcgtactcgg
     4981 tggcgggttc atcggcctgg agatcgcgtc ttctgcctgc aaaatgggta aacacgtcac
     5041 ggtgatcgag cgggcgccac gtctgctttc acgagtggtt agcgaggcat tcgcgacatt
     5101 tattggtgac attcatctgg gcaacggtat tgagcttcgt ctgggtgaag aagtgcgcga
     5161 ggtgaggcgg tgcaccagcg gtgtgcgggt cgatgccgtg tttttgagtg acggccaact
     5221 gctggaatgt gacatgttgg tcattggtgt cggttccgag ccacgcatgg agctggcaac
     5281 agcagctggc ctggcgtgcg cttccggcgt tctggtggat gagcactgtc acaccagcga
     5341 cccgttcatt agtgccatcg gtgactgtgt ggcagtgtgt ccttcgccag ggcatcagtt
     5401 accacgccgc gagagcgtgc agaacgctac agagcaggca cgtctggttg cagcacgtct
     5461 gagcggtcgg cccgtgccgc cagtacagac gccctggttc tggtccgatc aactgcaggc
     5521 gcgaataaat ctggcgggtg aacgtccggc gcaagggcag gtgattgtgc gtcgttacgg
     5581 cggcgacaag gtttcgatgc tgtacctgca agaccagcaa ttggtggcta ttgaagcctg
     5641 caacatgccg ggtgactgct tgctggcacg acgtgccatt ggtcagaacc acagcctcga
     5701 tctcgctcgt ctggttgacg ccgatgttcc gctgaaagac gcccttcatt tcgcatagga
     5761 gctaccaaca tgccacgtgt cgtgtttatc gatgagcaat ccggcgaata cgccgtagat
     5821 gcccaggacg ggcaatcgtt gatggaggtg gccacgcaga atggtgtgcc aggaatcgtc
     5881 gcggagtgcg gcggctcatg tgtctgcgct acctgccgga tcgagatcga agacgcatgg
     5941 gtcgagatcg tcggtgaagc caacccagat gagaacgatc ttttgcagag taccggcgaa
     6001 cccatgacgg ctggaacgcg attgtcctgc caggtgttca tcgacccctc catggacggt
     6061 ttgatcgtca gggttccact acctgcttag ggacgttttt acgtcgctcc cgcgaaagca
     6121 ggggccaggc actctcgggg tcttggattc tcgacctcgc gagagtgctc aatgctttag
     6181 aagctcttca gctactgcgt ttacatatta ccgcccaagc tgccggtcct gactggtggg
     6241 aagctgtgct gtgcgcgtta tcggcacagc tcaatgataa cttagaccaa aataagaaga
     6301 ggtcattgaa tgcgtagcct atctctgcga gtggaagttc ggtaactggt ggtgtcagcc
     6361 tggtgttcgt gcaattacga cattgttgtg ttgttactag ccaatcgttg cctggtgtgt
     6421 cgaggtctat cccggtgatt acacagcact gccgccaggt acgaaccttg gcgtgatcta
     6481 ttacgaaaca gccgagagaa ctcagttctg ggcgaggggg gacagacagg ctggagaccc
     6541 aaggctgaca gccagagtcg gcattgcacg ctacatcact ttatggaggc gaacggcgtc
     6601 ttgatcgatc cgagatttgg
//