From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Anabaena sp. (clone AnH20.1) nitrogen fixation operon nifB, fdxN,
Date: 15 Sep 92 10:02:09 GMT
Distribution: bionet
Checksum: 63245    13

LOCUS       ANANIFBH     5936 bp ds-DNA             BCT       15-SEP-1992
DEFINITION  Anabaena sp. (clone AnH20.1) nitrogen fixation operon nifB, fdxN,
            nifS, nifU, and nifH genes, complete cds.
ACCESSION   J05111 J01538 J05152 M21840 X05593 X05595
KEYWORDS    direct repeat; ferredoxin-like protein; nitrogen fixation protein;
            nitrogenase; nitrogenase reductase; rearranged DNA.
SOURCE      Anabaena sp. (library: PCC 7120) heterocyst DNA.
  ORGANISM  Anabaena sp.
            Prokaryotae; Gracilicutes; Oxyphotobacteria; Cyanobacteria;
            Nostocales; Nostocaceae.
REFERENCE   1  (bases 1 to 5936; 4666 to 5936)
  AUTHORS   Mevarech,M., Rice,D. and Haselkorn,R.
  TITLE     Nucleotide sequence of a cyanobacterial nifH gene coding for
            nitrogenase reductase
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 77, 6476-6480 (1980)
  STANDARD  full staff_review
REFERENCE   2  (bases 1 to 5936; 2327 to 2364)
  AUTHORS   Golden,J.W., Mulligan,M.E. and Haselkorn,R.
  TITLE     Different recombination site specificity of two developmentally
            regulated genome rearrangements
  JOURNAL   Nature 327, 526-529 (1987)
  STANDARD  simple automatic
REFERENCE   3  (bases 1 to 5936; 2038 to 2423)
  AUTHORS   Mulligan,M.E., Buikema,W.J. and Haselkorn,R.
  TITLE     Bacterial-type ferredoxin genes in the nitrogen fixation regions of
            the cyanobacterium Anabaena sp. strain PCC 7120 and Rhizobium
            meliloti
  JOURNAL   J. Bacteriol. 170, 4406-4410 (1988)
  STANDARD  simple staff_review
REFERENCE   4  (bases 1 to 5936; 1 to 2037; 2424 to 4748)
  AUTHORS   Mulligan,M.E. and Haselkorn,R.
  TITLE     Nitrogen fixation (nif) genes of the cyanobacterium Anabaena
            species strain PCC 7120
  JOURNAL   J. Biol. Chem. 264, 19200-19207 (1989)
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     mRNA            189..>1899
                     /gene="nifB"
     CDS             472..1899
                     /product="nitrogenase"
                     /gene="nifB"
                     /codon_start=1
                     /translation="MTPPVTGSSVTESTPTKAKSGGCGCDTSTTVEMDEKLQERIAKH
                     PCYSEEAHHHYARMHVAVAPACNIQCNYCNRKYDCANESRPGVVSELLTPEEAAHKVL
                     VIAGKIPQMTVLGIAGPGDPLANPEKTFRTFELIADKAPDIKLCLSTNGLMLPEYVDR
                     IKQLNIDHVTITLNTIDPEIGAQIYSWVHYKRRRYRGAEGARILLEKQMEGLQALREA
                     DILCKVNSVMIPGINDQHLVEVNKMIREQGAFLHNIMPLISAPEHGTHFGLTGQRGPS
                     QKELKSVQDQCSGNMKMMRHCRQCRADAVGLLGEDRSQEFTKDKFLEMAPEYDFDKRQ
                     EVHEGIEKFRVELKVAKEKVLAGKEKTANNPKILVAIATKGGGLVNQHFGHAKEFQVY
                     EVDGSEVSFVSHRKVDHYCQGGYGEEATFDNIVKTIADCKAVLVSKIGESPKEKLLQA
                     GIQTVEAYDVIEKVALEFYEQWNKG"
     repeat_region   1984..2032
                     /note="seven 7-bp imperfect direct repeats"
     CDS             2050..2400
                     /product="ferrodoxin-like protein"
                     /gene="fdxN"
                     /codon_start=1
                     /translation="MAYTITSQCISCKLCSSVCPTGAIKIAENGQHWIDSELCTNCVD
                     TVYTVPQCKAGCPTCDGCVKVPSDYWEGWFANYNRVIAKLTKKQDYWERWFNCYSQKF
                     SEQLQKHQGEILGV"
     misc_feature    2341..2345
                     /note="rearrangement breakpoint"
     CDS             2403..3605
                     /gene="nifS"
                     /codon_start=1
                     /translation="MSVIYLDNNATTKVDPDVVEAIMPYLTDYYGNPSSMHTFGGQLG
                     KAVRTAREQVAALLGADESEIVFTSCGTEGDNAAIRAALLAQPAKRHIITTQVEHPAV
                     LNVCKQLETQGYTVTYLSVNSHGQLDLDELEASLTGNTALVTIMYANNETGTVFPIEE
                     IGKRVKERGAIFHVDAVQAVGKIPLNMKTSTIDMLTISGHKIHAPKGIGALYVRRGVR
                     FRPLLIGGHQERGRRAGTENVPGIVGLGKAAELELIHIETAIKKETRLRDRLEQTLLA
                     KIPDCEVNGDITQRLPNTTNIGFKYIEGEAILLSLNKYGICASSGSACTSGSLEPSHV
                     LRAMGLPYTTLHGSIRFSLCRYTTEAQIDRVIEVMPEIVERLRALSPFKNDEAGWLQA
                     QEQTLAHR"
     repeat_region   3662..3703
                     /note="six 7-bp imperfect direct repeats"
     CDS             3737..4639
                     /gene="nifU"
                     /codon_start=1
                     /translation="MWDYTDKVLELFYDPKNQGVIEENGEPGVKVATGEVGSIACGDA
                     LRLHIKVEVESDKIVDSRFQTFGCTSAIASSSALTEMIKGLTLDEALKVSNKDIADYL
                     GGLPEAKMHCSVMGQEALEAAIYNYRGIPLAAHDEDDEGALVCTCFGVSENKVRRIVI
                     ENDLTDAEQVTNYIKAGGGCGSCLAKIDDIIKDVKENKAATNLNNKGGSKPTNIPNSG
                     QKRPLTNVQKIALIQKVLDEEVRPVLIADGGDVELYDVDGDIVKVVLQGACGSCSSST
                     ATLKIAIESRLRDRINPSLVVEAV"
     mRNA            4816..>5936
                     /gene="nifH"
     CDS             4860..5759
                     /product="nitrogenase reductase"
                     /gene="nifH"
                     /codon_start=1
                     /translation="MTDENIRQIAFYGKGGIGKSTTSQNTLAAMAEMGQRIMIVGCDP
                     KADSTRLMLHSKAQTTVLHLAAERGAVEDLELHEVMLTGFRGVKCVESGGPEPGVGCA
                     GRGIITAINFLEENGAYQDLDFVSYDVLGDVVCGGFAMPIREGKAQEIYIVTSGEMMA
                     MYAANNIARGILKYAHSGGVRLGGLICNSRKVDREDELIMNLAERLNTQMIHFVPRDN
                     IVQHAELRRMTVNEYAPDSNQGQEYRALAKKINNDKLTIPTPMEMDELEALKIEYGLL
                     DDDTKHSEIIGKPAEATNRSCRN"
BASE COUNT     1830 a   1389 c   1320 g   1397 t
ORIGIN
        1 cccctttcca taaatactcg taccccaact agaaaatact agaaccccta cctggaaaaa
       61 attttacaaa ttctcacaac gttctcgaaa tcatcatttg agattttgag tacaacttta
      121 ataaatccaa taaattctat tccagagata caaactgtaa tagtcaaaga cgactgctat
      181 aaatcttata tcgccaatca cttaacgctt ctacataaaa cgcaagcaaa aagtgagtgg
      241 agggatgaga tagcactatg ccatctggaa gagaagtcgc ggctttaaac agattctacc
      301 aagaccccaa gttgacacac accagccgaa accaaggact tcatagcctg ccctactcag
      361 cgaagccgct acaggcaatg tggcagacgc gacttcaatt cttagggcaa acacaatcag
      421 caacggcatc tcccacagtg gcagctagtc caccgacatt tgcgaaattc aatgacacca
      481 ccagttacag gctcttccgt tactgaatcg acacctacca aagcaaaatc aggtggttgc
      541 ggatgcgaca ccagcaccac cgtggaaatg gacgaaaagc tccaagaacg tattgctaaa
      601 catccctgct acagcgaaga ggctcaccac cattacgcga gaatgcacgt tgctgttgca
      661 cctgcctgca acattcaatg caactattgc aaccgaaaat atgattgtgc taacgaaagc
      721 cgtcctggcg tagttagtga attactcaca ccagaagaag cagcacacaa agtcttagta
      781 attgcaggca aaattcccca aatgacagtt ttgggaattg ctggccctgg cgatcctctg
      841 gcgaacccag aaaagacctt ccgcaccttt gagttgattg cagacaaagc gccagatatt
      901 aagctgtgtc tatcaactaa tggtttgatg ctgccggaat atgtcgatcg cattaaacaa
      961 ctaaatattg accacgttac tattacccta aatactattg acccagaaat cggcgcacag
     1021 atttattctt gggttcacta caagcgcaga cgttatagag gtgcggaagg cgcgagaatt
     1081 ctgctagaga agcagatgga aggcttacaa gctctcagag aagctgatat cttgtgtaaa
     1141 gttaattccg tgatgattcc gggcattaac gatcaacatc tggttgaagt caacaagatg
     1201 attcgtgaac aaggtgcatt cctgcacaac atcatgcctt tgatttctgc accagaacac
     1261 ggtacacact tcggcttaac tggtcaacgc gggccttcac aaaaagaact gaagtcagtg
     1321 caagaccaat gttctggcaa catgaaaatg atgcgccatt gccgccagtg ccgtgctgac
     1381 gctgtaggct tgttaggaga agaccgcagc caggaattta ccaaagataa attcctcgaa
     1441 atggctccag aatatgactt cgacaaacgt caagaagtcc acgaaggtat tgagaaattt
     1501 agagtagaac taaaagtagc caaagaaaaa gtactagctg gtaaagaaaa aacagctaac
     1561 aatcctaaaa tcttagttgc aatagcaacc aaaggcggcg gattagttaa ccaacacttc
     1621 ggccatgcca aggaatttca ggtttacgaa gtagacggta gtgaagttag cttcgtcagt
     1681 caccgcaagg ttgatcatta ttgtcaaggt ggatacggcg aagaagccac atttgacaat
     1741 attgttaaaa ctatcgcaga ttgtaaagca gttttggttt ccaagattgg cgaatctccc
     1801 aaagaaaaac tgctccaagc tggtatacag actgttgaag cttacgacgt gattgagaag
     1861 gttgctttag agttttacga gcaatggaat aagggctagg gagtagggac tggtgagcca
     1921 gcgcggtctt ggggtttccc ccataagcga ctggcgaacc ccgaaggggg gactaggcaa
     1981 attacccaat ccccaatccc cgatacctaa tccccaatct ccaatcccca atcaacaagg
     2041 agaataatca tggcttacac tatcaccagc caatgtattt cctgcaagct ctgttcgtct
     2101 gtatgcccca ctggtgcaat taaaatcgct gaaaacggac agcactggat tgactccgaa
     2161 ctgtgtacaa attgcgttga taccgtctac acagtcccac aatgtaaagc tggttgtcct
     2221 acttgcgatg gttgcgttaa agtacctagc gattattggg aaggctggtt tgctaactac
     2281 aaccgagtta tagcgaaatt gacaaaaaaa caagactatt gggaacgttg gtttaattgt
     2341 tattctcaga aattttccga acagctacaa aagcatcagg gtgaaatctt aggggtataa
     2401 agatgagtgt tatttatctc gataataatg ctaccactaa ggtagatcca gacgttgtag
     2461 aggcaatcat gccctacctg accgattatt acggcaatcc ttctagtatg cacacctttg
     2521 gtggacaact aggaaaggca gtcagaacag cgagagaaca agttgctgcc ctgttgggtg
     2581 cggatgaatc agaaattgtt tttaccagtt gtggaaccga gggtgataat gcggctattc
     2641 gcgccgcatt gttagcccaa cctgcgaagc gccacatcat tacaactcag gtagaacacc
     2701 ccgcagtctt aaatgtctgc aagcaattag aaacccaagg ctacactgtt acctatcttt
     2761 ccgtaaatag tcacgggcaa ttggatctag atgaactaga agcctcactg acaggtaaca
     2821 ccgccttggt gacaattatg tatgcgaaca acgaaaccgg gacagtcttc cccattgaag
     2881 aaattgggaa gcgagttaag gaacgtggcg caatcttcca tgtggatgcg gtgcaagcag
     2941 taggtaagat acctctgaat atgaaaacca gcaccatcga tatgttaacc atatctggtc
     3001 acaaaatcca tgcacccaag ggtattggcg ctttgtatgt gcgtcgaggt gtgagattcc
     3061 gtcccttgct gattggtgga caccaagaac gtggtcgtcg tgcaggaaca gaaaatgtac
     3121 cgggaattgt cggtttagga aaagccgcag agttagaatt aattcatatc gaaacagcga
     3181 ttaaaaaaga aacaaggctg cgcgatcgcc tagaacaaac cttactcgct aaaatccctg
     3241 actgcgaagt taacggcgat attacgcaga gattgcccaa caccaccaac atcggcttca
     3301 aatacatcga aggcgaagcg attctgctct ccttgaacaa atatggcatc tgtgcgtcgt
     3361 ctggttcggc ttgtacctct gggtcgctgg aaccatccca cgtcctccgg gcaatgggtt
     3421 taccatacac caccctccac ggttcgattc gcttcagtct ttgtcgctac actacagagg
     3481 ctcaaatcga tcgcgtcatc gaagtcatgc ccgaaattgt cgaacgtctc cgcgcccttt
     3541 ccccctttaa aaatgatgaa gcgggttggt tgcaagccca agaacagaca ttggcacatc
     3601 gttaattagg gaataggaag aagctgaggg gcagggagca gagggaaaaa atatctataa
     3661 ttcccaattc ccagtaccca gcccccagta cccaatcccc cattcagcaa taattattaa
     3721 gcactcggaa ctaaatatgt gggactacac agataaagta ttagaactgt tttacgatcc
     3781 caagaaccag ggagtcatcg aagaaaacgg cgaacctggc gtgaaggttg ctacaggaga
     3841 agtagggagt attgcttgcg gtgatgcgct gagactgcac atcaaggtag aagtagaatc
     3901 cgataagatt gttgactctc gcttccaaac ttttggctgc accagtgcga tcgcttcctc
     3961 tagcgctttg acagaaatga ttaagggttt gactttagat gaagccctga aagtttctaa
     4021 taaagacatt gctgattacc tcggtggctt gccagaagcc aaaatgcact gctctgttat
     4081 ggggcaggaa gctttggaag ctgctatcta taattatcgc ggtattcctc tcgccgccca
     4141 tgatgaagat gacgaaggtg cattagtttg tacttgcttc ggtgtgagtg aaaataaggt
     4201 acgtcgcata gttatcgaaa atgaccttac tgatgcggaa caggtcacaa attacattaa
     4261 agctggcggc ggttgcggtt cctgtttagc taaaattgat gatatcatta aagatgtaaa
     4321 ggaaaacaaa gccgcaacaa acctcaacaa taaaggcgga agtaaaccta caaatattcc
     4381 taattctggg cagaaacgac cgttaaccaa cgtgcagaag atagccctca tccaaaaagt
     4441 attagacgaa gaagtaagac ccgtattgat tgccgacggc ggagatgtag aactctacga
     4501 tgtagacggc gatattgtca aagtagtact gcaaggggcg tgtggctctt gttctagtag
     4561 tacagccacc ttaaagatag cgattgaatc cagattacgc gatcgcatca accccagcct
     4621 agtagtagaa gcagtttagt taacagttaa cagtaaaccg ttaactaaca cccaaaagaa
     4681 ctttcacaac tacataacga acccatcatg aacactaatt ctactggttt ttctgtggag
     4741 cgatcgcccc ctcttcggcg actgttctac ataacccctc acagccatag ctcaaacagg
     4801 cgtgagatcc aaacacaaag accgaccaac taaccaacca attgcaggaa aagagaacaa
     4861 tgactgacga aaacattaga cagatagctt tctacggtaa aggcggtatc ggtaaatcta
     4921 ccacctccca aaacaccctt gcagctatgg cagaaatggg tcaacgcatc atgattgtag
     4981 gttgcgaccc taaagctgac tccacccgtc tgatgcttca ctccaaagct caaaccaccg
     5041 tactacactt agctgctgaa cgcggtgcag tagaagactt agaactccac gaagtaatgt
     5101 tgaccggttt ccgtggcgtt aagtgcgtag aatctggtgg tccagaaccc ggtgtaggtt
     5161 gcgccggtcg tggtatcatc accgccatta acttcttaga agaaaacggc gcttaccaag
     5221 acctagactt cgtatcctac gacgtattgg gtgacgttgt atgtggtggt ttcgctatgc
     5281 ctatccgtga aggtaaagca caagaaatct acatcgttac ctctggtgaa atgatggcga
     5341 tgtatgctgc taacaacatc gctcgcggta ttttgaaata tgctcactcc ggtggtgtac
     5401 gtttaggtgg tttgatctgt aacagccgta aggttgaccg tgaagacgag ttaatcatga
     5461 acttggctga acgtttgaac acccaaatga ttcacttcgt acctcgtgac aacatcgttc
     5521 aacacgcaga attgcgccgt atgaccgtta acgagtacgc accagacagc aaccaaggtc
     5581 aagagtaccg cgcattagct aagaagatca acaacgacaa gctcaccatt cctacaccaa
     5641 tggaaatgga tgaactagaa gctctgaaga tcgaatacgg tctattagac gacgacacca
     5701 agcactctga aatcatcggt aagcccgcag aagctaccaa taggtcatgc cgtaattagg
     5761 agacacggag acaggagatg aggagcaatt cctcttccca ctctcccttc ccgactcctc
     5821 actctcccaa atatacttct attcccccat tcgtaagagt cactgaggca gatatgacac
     5881 ctcctgaaaa caagaatctt gtagatgaaa ataaggaact tattcaagaa gttctg
//