From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Medicago sativa NADH-glutamate synthase mRNA, comlete cds.
Date: 29 Dec 92 19:56:35 GMT
Distribution: bionet
Checksum: 26367 14

LOCUS       ALFNADHSYN   7174 bp ss-mRNA            PLN       10-DEC-1992
DEFINITION  Medicago sativa NADH-glutamate synthase mRNA, comlete cds.
ACCESSION   L01660 L01661
KEYWORDS    .
SOURCE      Medicago sativa (strain Saranac) (library: Lambda gt22) root nodule
            cDNA to mRNA.
  ORGANISM  Medicago sativa
            Eukaryota; Plantae; Embryobionta; Magnoliophyta; Magnoliopsida;
            Rosidae; Fabales; Fabaceae.
REFERENCE   1  (bases 1 to 7174)
  AUTHORS   Gregerson,R.G., Miller,S.S., Twary,S.N., Gantt,J.S. and Vance,C.P.
  TITLE     Molecular characterization of NADH-glutamate synthase from alfalfa
            nodules
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     5'UTR           <1..227
                     /evidence="EXPERIMENTAL"
                     /citation=[1]
                     /note="stop codons exist in all three reading frames in
                     this region"
     3'UTR           6813..7174
                     /evidence="EXPERIMENTAL"
                     /citation=[1]
     polyA_site      7174
     CDS             228..6812
                     /EC_number="1.4.1.14"
                     /standard_name="NADH-GOGAT"
                     /evidence="EXPERIMENTAL"
                     /citation=[1]
                     /note="activity increases dramatically during nodule
                     development"
                     /product="NADH-glutamate synthase"
                     /codon_start=1
                     /translation="MSNSLSLTFTALNNPQINAISNPNARLRPLARVTRCSATCVERK
                     RWLGTKLRSGGGLERIQLWESGGLGRLPKLRVAVKSSFSAVPDKPMGLYDPAFDKDSC
                     GVGFVAELNGQSSRKTVTDALEMLVRMTHRGACGCEANTGDGAGILVALPHGFYQEVV
                     DFQLPPQGNYAVGMFFLPKSDSRRKESKNIFTKVAESLGHKVLGWRSVPTDNTGLGKS
                     AQLTEPVIEQVFLTPSSDSKVDLEKQMYILRKLSMVSITSALNLQSDGITDFYICSLS
                     SRTVIYKGQLTPAQLGEYYYADLGNERFTSYMALIHSRFSTNTFPSWDRAQPFRVLGH
                     NGEINTLRGNVNWIKAREGLLKCKELGLSENDLKKFLPIVDANSSDSGCFDGVLEFLL
                     HSGKSLPEAVMMMIPEAWQNDKNMDPQRKAFYEYYSALMEPWDGPALISFTDGHYLGA
                     TLDRNGLRPGRFYVTHSGRVIMASEVGVVDIPPEDVCRKGRLNPGMMLLVDFEKQIVV
                     NDDALKEQYSLARPYGDWLEKQKIELKDIIDSVHESDIVPPTISGVPPLSNDDVDMEN
                     MGIQGLLAPLKAFGYSVESLEILLLPMAKDGVEALGSMGNDTPLAVMSNREKLTFEYF
                     KQMFAQVTNPPIDPIREKIVTSMRCMVGPEGDLTETTEEQCHRLSLKGPLLSTKEMEA
                     IKKMNYRGWRSKVIDITYSKERGTKGLEEALDRICTEAHNAISEGYTTLVLSDRAFSK
                     KHVAVSSLLAVGAVHQHLVKTLERTRVALMVESAEPREVHHFCTLVGFGADAICPYLA
                     IEAIWRLQVDGKIPPKASGDFNSKDELVKKYFKASTYGMMKVLAKMGISTLASYKGAQ
                     IFEALGLSSEVIEKCFAGTPSRVEGATFEMLAQDALHLHELAFPSRIFSPGSAEAVAL
                     PNPGDYHWRKGGEVHLNDPLAIAKLQEAARTNSVDAYKQYSKTIHELNKACNLRGLLK
                     FKDAASKVPISEVEPASEIVKRFCTGAMSYGSISLEAHTALATAMNTIGGKSNTGEGG
                     EQPSRMEPLADGSRNPKRSAIKQVASGRFGVSSYYLTNADELQIKMAQGAKPGEGGEL
                     PGHKVIGDIAITRNSTAGVGLISPPPHHDIYSIEDLAQLIHDLKNANPAARISVKLVS
                     EAGVGVIASGVVKGHAEHVLISGHDGGTGASRWTGIKSAGLPWELGLAETHQTLVAND
                     LRGRTTLQTDGQLKTGRDVAIAALLGAEEYGFSTAPLITLGCIMMRKCHKNTCPVGIA
                     TQDPVLREKFAGEPEHVINFFFMVAEEMREIMSQLGFRTVNEMVGRSDMLEVDKEVVK
                     GNAKLENIDLSLLLRPAAELRPEAAQYCVQKQDHGLDMALDNKLISLSNAALEKGLPV
                     YIETPICNTNRAVGTMLSHEVTKRYNLAGLPADTIHIQFTGSAGQSFGAFLCPGITLE
                     LEGDSNDYIGKGLSGGKVVVYPPKGSNFDPKDNILIGNVALYGATRGEAYFNGMAAER
                     FCVRNSGALAVVEGVGDHGCEYMTGGTVVVLGKTGRNFAAGMSGGIAYVLDVDGTFQS
                     RCNLELVDLDKVEEEEDIITLRMLIQQHQRHTNSLLAKEVLVDFENLLPKFVKVFPRE
                     YKRVLASMKSDAASKDAVERAAEDVDEQDDEAQAVEKDAFEELKKLATASLNEKPSEA
                     PKRPSQVTDAVKHRGFVAYEREGVQYRDPNVRLNDWNEVMMETKPGPLLKTQSARCMD
                     CGTPFCHQENSGCPLGNKIPEFNELVYQNRWQEALERLLETNNFPEFTGRVCPAPCEG
                     SCVLGIIENPVSIKNIECAIIDKAFEEGWMIPRPPVKRTGKRVAIVGSGPSGLAAADQ
                     LNKMGHIVTVFERADRIGGLMMYGVPNMKTDKVDIVQRRVNLMAEEGINFVVNANIGL
                     DPLYSLERLREENDAIVLAVGATKPRDLPVPGRELSGVHFAMEFLHANTKSLLDSNLQ
                     DGNYISAKGKKVVVIGGGDTGTDCIGTSIRHGCTAVVNLELLPQPPPTRAPGNPWPQW
                     PRIFRVDYGHQEAETKFGKDPRTYEVLTKRFVGDENGVVKGLEVVRVCWEKDETGKFQ
                     FKEIEGSEEIIEADLVLLAMGFLGPEATIAEKLGVERDNRSNFKADYGRFSTSVDGVF
                     AAGDCRRGQSLVVWAISEGRQAAAQVDSYLTNEDHGIDGNQDEFVKRQQDLNKKHSKH
                     TVMT"
BASE COUNT     2060 a   1313 c   1790 g   2011 t
ORIGIN      
        1 ctctgtcttg tttttttctc tcttgttgct gttttagttt tttcagtttt tcagattcga
       61 atctccacat aaccacttct caaaaatctg gatcttattg ttcttcagtt tcatcaatct
      121 cagatctgtt acatttaata acaaacaaaa ctgaataaca gttagaaaaa gccaaaactc
      181 gaaaacgcga atcaacaatt tttgaataat tttaatttta atctccaatg tcgaattctc
      241 tttcgttaac gtttacggcg ttaaacaatc ctcagataaa cgctataagt aaccctaacg
      301 ctcgtttgcg tccgttagcg agggttacgc gatgttctgc cacgtgtgtc gagaggaaga
      361 gatggttagg gacgaaattg cgaagtggtg gtggtttgga gaggattcag ttatgggaat
      421 ccggtggatt agggaggttg ccgaagctca gagtggcggt taagtcgtcg ttttcggctg
      481 tgccggataa accgatggga ctttatgatc cggcttttga taaagattct tgtggtgttg
      541 gatttgttgc tgaattgaac ggtcaaagta gtcgaaaaac ggtaactgat gctttggaga
      601 tgttggttag gatgacacat agaggtgctt gtggatgtga agcaaatacc ggtgatggtg
      661 ctggtatact tgtggctttg cctcatggct tctaccaaga ggttgtggat tttcagcttc
      721 ctcctcaagg aaactatgca gttggcatgt ttttcttacc taaatccgat agtcggagaa
      781 aggaaagcaa aaatatattt actaaggttg ccgagtctct tggtcacaag gttcttggtt
      841 ggcgttctgt gcccacggat aacacagggt tgggcaaatc agcccagctg accgaaccag
      901 tgattgaaca agtgtttctt acaccaagtt ctgactcaaa agttgatttg gagaaacaga
      961 tgtacatatt aaggaagctc agcatggttt ctattacatc tgcattaaac ctccaaagcg
     1021 atggaattac agatttctat atctgttcac tttcatctag gactgttatt tacaaaggtc
     1081 agctaacacc agctcagttg ggtgaatatt attatgcaga tcttggcaat gaaaggttta
     1141 cgagctacat ggccctgata cattcccgat tctctacaaa tactttccct agttgggatc
     1201 gtgcccaacc ttttcgtgta ttgggccaca acggggaaat caacacactc agaggcaatg
     1261 ttaactggat aaaagcacgt gagggcctac tgaaatgcaa ggaacttggc ttatcagaga
     1321 atgatttaaa gaagttttta ccgattgtgg acgcaaattc atcagattca ggatgttttg
     1381 atggtgtcct cgagtttttg cttcattccg gaaaaagtct tccagaagct gttatgatga
     1441 tgattcctga agcatggcag aacgacaaga acatggaccc tcagcgtaaa gcattttatg
     1501 aatactattc agctctgatg gagccatggg atgggccagc tcttatctca tttaccgatg
     1561 gtcactatct aggagctaca ttagatagga atggactacg acctggccgc ttctatgtca
     1621 cccacagtgg acgggttata atggcaagtg aagttggcgt tgtagatatt cctccagaag
     1681 atgtgtgcag gaaaggaaga ctaaatccag gcatgatgct tctggtggat tttgagaagc
     1741 aaatagttgt gaacgatgac gccttaaaag agcaatactc attggcaaga ccgtacggag
     1801 attggcttga aaagcagaaa atcgaactga aggacataat tgactctgtt catgaatctg
     1861 atattgtgcc accaaccata tcaggagtgc ctccactgtc taatgatgat gtggacatgg
     1921 aaaatatggg aattcaaggt ttgctggctc cattgaaagc ttttgggtat tctgtagaat
     1981 cattggaaat tctattactt cccatggcaa aggatggtgt cgaagcactt ggttcaatgg
     2041 gaaatgatac tccattagct gtcatgtcta atagagaaaa actcactttt gagtatttca
     2101 aacaaatgtt tgctcaagtg acaaatcctc ctatcgatcc tattcgagag aaaatagtca
     2161 cttctatgcg atgtatggtt ggtccagaag gtgatctaac agaaaccact gaggaacaat
     2221 gccaccgcct ttcactaaaa ggtccccttt tatccactaa ggaaatggaa gccatcaaaa
     2281 aaatgaatta taggggatgg aggagcaaag ttatagacat tacttactca aaggaacgtg
     2341 gtacgaaagg gttggaggaa gccttagaca ggatatgcac agaagcacac aatgcaatta
     2401 gtgaaggcta caccaccctt gttctttctg atagagcctt ctcaaagaaa catgttgctg
     2461 tgagctctct tttggctgtt ggtgcagtcc accaacatct agttaaaaca cttgagcgca
     2521 caagagttgc tctaatggtt gaatctgctg agccacgtga agtgcaccat ttctgcacac
     2581 ttgttggttt tggcgctgat gctatatgcc catatttggc tatagaggca atttggcgac
     2641 tgcaggttga cggaaagatc ccaccaaaag caagtggtga tttcaactca aaagatgagt
     2701 tggtcaagaa gtatttcaaa gcaagcacct atgggatgat gaaggttctt gccaagatgg
     2761 gaatatcaac tttggcatca tataaaggtg ctcagatatt tgaagctctg ggtctttctt
     2821 cagaagtgat tgaaaagtgc tttgctggaa ctccaagtcg agttgagggt gcaacatttg
     2881 agatgcttgc tcaagatgca cttcatctgc atgagttggc ttttccttct cggattttct
     2941 ctcctggaag tgcagaagca gtagcattgc caaaccctgg agattatcat tggagaaaag
     3001 gtggtgaagt tcatctgaat gatccacttg ctattgcaaa gcttcaagag gctgccagaa
     3061 ctaacagcgt agatgcatat aaacagtatt ccaagaccat tcatgaattg aataaggctt
     3121 gcaatttgcg aggactcctg aagtttaaag atgcagcaag taaggttcct attagcgaag
     3181 tcgaacctgc tagtgaaatt gttaaacggt tttgcactgg cgccatgagt tatgggtcaa
     3241 tatctttgga ggcccacaca gcattagcaa cggcaatgaa cacaattgga ggaaaatcca
     3301 acacaggtga gggaggtgaa caaccatctc gtatggagcc tcttgctgat ggctcaagga
     3361 atcccaaaag gagtgccatt aagcaggttg ctagtgggag atttggtgtt tcaagttact
     3421 accttacaaa tgctgatgag cttcagataa agatggccca gggagcaaaa cctggtgaag
     3481 gaggtgaact tcctggccac aaggttatag gagacattgc tatcactagg aattcaacag
     3541 ccggagtagg tctcatcagt ccacctcccc atcacgatat ttattcaatt gaagatcttg
     3601 ctcaattgat tcatgatctc aagaatgcca acccagctgc tcgaattagt gtgaaactag
     3661 tatctgaagc aggagttggg gtaattgcta gtggagttgt taaaggtcat gctgagcatg
     3721 tcttgatctc aggtcatgat ggaggtacag gagcatccag atggactggt ataaagagtg
     3781 ctggactgcc ttgggaactt ggtctggctg agacccacca aacattggtt gctaatgacc
     3841 tacgtggtcg tacaactctg caaactgacg ggcaactaaa aaccggaaga gatgtggcca
     3901 tagccgccct tcttggtgca gaagagtatg gtttcagtac tgctcccctc attactcttg
     3961 gctgcatcat gatgcggaag tgccacaaga acacctgtcc tgttggaatt gctactcaag
     4021 atccagtact tagagagaaa tttgctggag aacctgagca cgttattaac ttctttttta
     4081 tggtggcaga agagatgaga gaaattatgt cccaacttgg gtttagaaca gtcaatgaaa
     4141 tggttggccg ttcagatatg ctcgaagttg ataaagaagt tgttaaggga aatgcaaaac
     4201 tagagaatat tgatctctct ctattgctta gacctgcagc tgaactgcgg ccagaagctg
     4261 cccaatactg tgttcaaaag caagatcatg gtttggacat ggctttggat aataagctca
     4321 taagtctgtc caatgctgct ttggaaaagg gtcttccagt atacattgaa acaccgatct
     4381 gcaataccaa ccgtgctgtg ggaactatgc ttagccacga ggttactaag aggtacaatt
     4441 tagctggtct tccagccgat accatccata tccaatttac tggtagtgca ggccaaagct
     4501 ttggtgcatt cctttgtcct ggaatcacat tggaacttga aggtgatagc aatgactaca
     4561 ttggtaaagg attatcaggt ggaaaagttg tagtatatcc tccaaaagga agcaattttg
     4621 accccaaaga caacatctta attggcaatg tggcactgta tggggcaaca cgtggtgagg
     4681 catatttcaa tgggatggca gcagaaagat tttgtgtgcg taattctggg gctctggctg
     4741 tagttgaagg tgttggtgat catggatgtg agtacatgac tggtgggacc gttgttgttc
     4801 ttgggaaaac tggtagaaat tttgctgcag gtatgagtgg tggaatcgct tatgttcttg
     4861 acgtggatgg aacattccag tctcggtgca acctggagct tgtagatctg gataaggttg
     4921 aagaggaaga ggacattatt actcttagga tgttgataca gcaacatcaa cgtcacacaa
     4981 acagtttgct tgccaaggaa gtgcttgttg attttgaaaa tcttcttcct aaatttgtca
     5041 aggtgttccc tcgggagtat aaacgtgttc ttgcaagtat gaagtctgat gcagcctcta
     5101 aagatgcagt ggagcgggct gcggaagatg tagatgaaca agacgatgaa gcccaagcag
     5161 tggagaaaga tgcttttgaa gagctcaaga agctggcaac tgcatctttg aatgagaaac
     5221 caagtgaggc tcccaagagg ccaagtcagg tcactgatgc tgttaaacat agaggttttg
     5281 ttgcttacga gcgtgagggt gttcagtata gggatcctaa tgttcgcctg aatgattgga
     5341 atgaggtgat gatggagaca aagccgggtc cacttttgaa aacacagtct gcccgttgca
     5401 tggactgtgg tactccattt tgtcatcagg aaaattctgg atgtcctctt gggaacaaaa
     5461 taccggaatt taatgagcta gtgtatcaaa ataggtggca agaagcacta gagaggcttc
     5521 ttgaaacaaa caactttcct gagttcactg ggcgggtttg ccctgcacct tgtgaaggtt
     5581 cttgtgtcct cggtattatt gagaatccag tttctattaa aaatattgaa tgtgctatca
     5641 ttgacaaggc ttttgaggag ggttggatga ttccacgacc tcctgtcaag agaactggaa
     5701 aaagagtagc cattgttgga agtggaccat caggcttggc agctgcagat cagctaaata
     5761 aaatgggtca tatagtaaca gtgtttgaaa gagctgatcg cattggtggc cttatgatgt
     5821 atggagttcc aaacatgaaa actgacaaag tggatatagt tcaaaggcga gtcaatctaa
     5881 tggctgagga aggaataaat tttgtggtga atgcaaacat tgggcttgat cctttatact
     5941 ctcttgaacg acttagagag gaaaacgatg ccattgtctt ggctgttgga gctacaaagc
     6001 caagggatct tcctgtgcct ggaagggagc tatcaggagt tcattttgcc atggagtttc
     6061 ttcatgcaaa cactaaaagt ttgcttgata gcaatttaca ggatggtaac tacatctctg
     6121 ccaagggcaa gaaggttgtg gtcattggcg ggggtgacac gggcacagat tgcattggaa
     6181 catccattcg tcatggttgt actgctgttg tcaatctaga acttctccct cagccaccac
     6241 caactagggc cccaggcaac ccatggcctc agtggcctcg catattccgt gttgattatg
     6301 gtcaccaaga agcggaaact aaatttggaa aagatcccag aacatatgag gtactcacta
     6361 agaggtttgt gggagatgaa aatggagttg ttaaaggact tgaagtggta cgtgtttgct
     6421 gggagaaaga tgaaactggc aagtttcaat ttaaagaaat tgagggcagt gaggagatta
     6481 ttgaggctga cctagtttta ctagccatgg gattccttgg ccccgaggcg actatagcag
     6541 aaaagttggg tgtggaacga gacaacaggt caaacttcaa agctgattat ggccgcttct
     6601 cgaccagtgt tgatggggta tttgcagcag gtgactgtcg gcgtggtcag tcgcttgtgg
     6661 tatgggctat ttcagaggga aggcaagctg ccgcacaagt ggacagctac ctcaccaatg
     6721 aagaccacgg tattgatggg aatcaggatg aatttgtcaa gaggcaacaa gacctcaaca
     6781 agaagcacag caaacacaca gtgatgactt aggatgcttt tttgatttta caagttcctc
     6841 gagttcctaa agcaaatgca cataataaaa gaaaaaggaa tgttgaatgt tacattttag
     6901 gcattcttgg ttggcaggta gtaagccgaa tgggcgaaat aagaacgaaa acaataaaga
     6961 taaataattt agtgtaagga aagcaagagg ttgtcttggt ttttggtggt attagggtaa
     7021 gatttgagtg ttgttcatag ttcttatttg gattttattg ttgggaaggc tagcttagtt
     7081 atggttttgt ggaaatgaaa tgtacagttt ggtaattaat tatttattct gatgtacaat
     7141 tgtttctgca attttgagct actgtgtttg ttcc
//