From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Lelystad virus RNA polymerase (ORF1a), ORF3, ORF4, ORF5, membrane
Date: 21 Dec 92 20:48:08 GMT
Distribution: bionet
Checksum: 16189 28

LOCUS       LEYPOLYENV  15101 bp ss-RNA             VRL       17-NOV-1992
DEFINITION  Lelystad virus RNA polymerase (ORF1a), ORF3, ORF4, ORF5, membrane
            protein (ORF6) and nucleocapsid protein (ORF7) genomic RNA,
            complete cds; RNA polymerase (ORF1b) and ORF2 genomic RNA, 3' end
            cds.
ACCESSION   M96262
KEYWORDS    RNA polymerase; envelope protein; nucleocapsid protein.
SOURCE      Lelystad virus cDNA to genomic RNA.
  ORGANISM  Lelystad virus
            Viridae; Nonclassified viruses.
REFERENCE   1  (bases 1 to 15101)
  AUTHORS   Meulenberg,J.J., Hulst,M.M., de Meijer,E.J., Moonen,P.L., den
            Besten,A., de Kluyver,E.P., Wensvoort,G. and Moorman,R.J.
  TITLE     Lelystad Virus, the causative agent of porcine epidemic abortion
            and respiratory syndrome (PEARS), is related to LDV and EAV
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     CDS             <7384..11775
                     /standard_name="ORF1b"
                     /product="RNA polymerase"
                     /codon_start=1
                     /translation="TGFKLLAASGLTRCGRGGLVVTETAVKIIKYHSRTFTLGPLDLK
                     VTSEVEVKKSTEQGHAVVANLCSGVILMRPHPPSLVDVLLKPGLDTIPGIQPGHGAGN
                     MGVDGSIWDFETAPTKAELELSKQIIQACEVRRGDAPNLQLPYKLYPVRGDPERHKGR
                     LINTRFGDLPYKTPQDTKSAIHAACCLHPNGAPVSDGKSTLGTTLQHGFELYVPTVPY
                     SVMEYLDSRPDTPFMCTKHGTSKAAAEDLQKYDLSTQGFVLPGVLRLVRRFIFGHIGK
                     APPLFLPSTYPAKNSMAGINGQRFPTKDVQSIPEIDEMCARAVKENWQTVTPCTLKKQ
                     YCSKPKTRTILGTNNFIALAHRSALSGVTQAFMKKAWKSPIALGKNKFKELHCTVAGR
                     CLEADLASCDRSTPAIVRWFVANLLYELAGCEEYLPSYVLNCCHDLVATQDGAFTKRG
                     GLSSGDPVTSVSNTVYSLVIYAQHMVLSALKMGHEIGLKFLEEQLKFEDLLEIQPMLV
                     YSDDLVLYAERPTFPNYHWWVEHLDLMLGFRTDPKKTVITDKPSFLGCRIEAGRQLVP
                     NRDRILAALAYHMKAQNASEYYASAAAILMDSCACIDHDPEWYEDLICGIARCARQDG
                     YSFPGPAFFMSMWEKLRSHNEGKKFRHCGICDAKADYASACGLDLCLFHSHFHQHCPV
                     TLSCGHHAGSKECSQCQSPVGAGRSPLDAVLKQIPYKPPRTVIMKVGNKTTALDPGRY
                     QSRRGLVAVKRGIAGNEVDLSDGDYQVVPLLPTCKDINMVKVACNVLLSKFIVGPPGS
                     GKTTWLLSQVQDDDVIYTPTHQTMFDIVSALKVCRYSIPGASGLPFPPPARSGPWVRL
                     IASGHVPGRVSYLDEAGYCNHLDILRLLSKTPLVCLGDLQQLHPVGFDSYCYVFDQMP
                     QKQLTTIYRFGPNICARIQPCYREKLESKARNTRVVFTTRPVAFGQVLTPYHKDRIGS
                     AITIDSSQGATFDIVTLHLPSPKSLNKSRALVAITRARHGLFIYDPHNQLQEFFNLTP
                     ERTDCNLVFSRGDELVVLNADNAVTTVAKALETGPSRFRVSDPRCKSLLAACSASLEG
                     SCMPLPQVAHNLGFYFSPDSPTFAPLPKELAPHWPVVTHQNNRAWPDRLVASMRPIDA
                     RYSKPMVGAGYVVGPSTFLGTPGVVSYYLTLYIRGEPQALPETLVSTGRIATDCREYL
                     DAAEEEAAKELPHAFIGDVKGTTVGGCHHITSKYLPRSLPKDSVAVVGVSSPGRAAKA
                     VCTLTDVYLPELRPYLQPETASKCWKLKLDFRDVRLMVWKGATAYFQLEGLTWSALPD
                     YARFIQLPKDAVVYIDPCIGPATANRKVVRTTDWRADLAVTPYDYGAQNILTTAWFED
                     LGPQWKILGLQPFRRAFGFENTEDWAILARRMNDGKDYTDYNWNCVRERPHAIYGRAR
                     DHTYHFAPGTELQVELGKPRLPPGQVP"
     CDS             <11772..11786
                     /standard_name="ORF2"
                     /product="ORF2"
                     /codon_start=1
                     /translation="VNSG"
     CDS             212..7402
                     /standard_name="ORF1a"
                     /product="RNA polymerase"
                     /codon_start=1
                     /translation="MSGTFSRCMCTPAARVFWNAGQVFCTRCLSARSLLSPELQDTDL
                     GAVGLFYKPRDKLHWKVPIGIPQVECTPSGCCWLSAVFPLARMTSGNHNFLQRLVKVA
                     DVLYRDGCLAPRHLRELQVYERGCNWYPITGPVPGMGLFANSMHVSDQPFPGATHVLT
                     NSPLPQQACRQPFCPFEEAHSSVYRWKKFVVFTDSSLNGRSRMMWTPESDDSAALEVL
                     PPELERQVEILIRSFPAHHPVDLADWELTESPENGFSFNTSHSCGHLVQNPDVFDGKC
                     WLSCFLGQSVEVRCHEEHLADAFGYQTKWGVHGKYLQRRLQVRGIRAVVDPDGPIHVE
                     ALSCPQSWIRHLTLDDDVTPGFVRLTSLRIVPNTEPTTSRIFRFGAHKWYGAAGKRAR
                     AKRAAKSEKDSAPTPKVALPVPTCGITTYSPPTDGSCGWHVLAAIMNRMINGDFTSPL
                     TQYNRPEDDWASDYDLVQAIQCLRLPATVVRNRACPNAKYLIKLNGVHWEVEVRSGMA
                     PRSLSRECVVGVCSEGCVAPPYPADGLPKRALEALASAYRLPSDCVSSGIADFLANPP
                     PQEFWTLDKMLTSPSPERSGFSSLYKLLLEVVPQKCGATEGAFIYAVERMLKDCPSSK
                     QAMALLAKIKVPSSKAPSVSLDECFPTDVLADFEPASQERPQSSGAAVVLCSPDAKEF
                     EEAAPEEVQESGHKAVHSALLAEGPNNEQVQVVAGEQLKLGGCGLAVGNAHEGALVSA
                     GLINLVGGNLSPSDPMKENMLNSREDEPLDLSQPAPASTTTLVREQTPDNPGSDAGAL
                     PVTVREFVPTGPILCHVEHCGTESGDSSSPLDLSDAQTLDQPLNLSLAAWPVRATASD
                     PGWVHGRREPVFVKPRNAFSDGDSALQFGELSESSSVIEFDRTKDAPVVDAPVDLTTS
                     NEALSVVDPFEFAELKRPRFSAQALIDRGGPLADVHAKIKNRVYEQCLQACEPGSRAT
                     PATREWLDKMWDRVDMKTWRCTSQFQAGRILASLKFLPDMIQDTPPPVPRKNRASDNA
                     GLKQLVAQWDRKLSVTPPPKPVGPVLDQIVPPPTDIQQEDVTPSDGPPHAPDFPSRVS
                     TGGSWKGLMLSGTRLAGSISQRLMTWVFEVFSHLPAFMLTLFSPRGSMAPGDWLFAGV
                     VLLALLLCRSYPILGCLPLLGVFSGSLRRVRLGVFGSWMAFAVFLFSTPSNPVGSSCD
                     HDSPECHAELLALEQRQLWEPVRGLVVGPSGLLCVILGKLLGGSRYLWHVLLRLCMLA
                     DLALSLVYVVSQGRCHKCWGKCIRTAPAEVALNVFPFSRATRVSLVSLCDRFQTPKGV
                     DPVHLATGWRGCWRGESPIHQPHQKPIAYANLDEKKMSAQTVVAVPYDPSQAIKCLKV
                     LQAGGAIVDQPTPEVVRVSEIPFSAPFFPKVPVNPDCRVVVDSDTFVAAVRCGYSTAQ
                     LVLGRGNFAKLNQTPPRNSISTKTTGGASYTLAVAQVSAWTLVHFILGLWFTSPQVCG
                     RGTADPWCSNPFSYPTYGPGVVCSSRLCVSADGVTLPLFSAVAQLSGREVGIFILVLV
                     SLTALAHRMALKADMLVVFSAFCAYAWPMSSWLICFFPILLKWVTLHPLTMLWVHSFL
                     VFCLPAAGILSLGITGLLWAIGRFTQVAGIITPYDIHQYTSGPRGAAAVATAPEGTYM
                     AAVRRAALTGRTLIFTPSAVGSLLEGAFRTHKPCLNTVNVVGSSLGSGGVFTIDGRRT
                     VVTAAHVLNGDTARVTGDSYNRMHTFKTNGDYAWSHADDWQGVAPVVKVAKGYRGRAY
                     WQTSTGVEPGIIGEGFAFCFTNCGDSGSPVISESGDLIGIHTGSNKLGSGLVTTPEGE
                     TCTIKETKLSDLSRHFAGPSVPLGDIKLSPAIIPDVTSIPSDLASLLASVPVVEGGLS
                     TVQLLCVFFLLWRMMGHAWTPIVAVGFFLLNEILPAVLVRAVFSFALFVLAWATPWSA
                     QVLMIRLLTASLNRNKLSLAFYALGGVVGLAAEIGTFAGRLSELSQALSTYCFLPRVL
                     AMTSCVPTIIIGGLHTLGVILWLFKYRCLHNMLVGDGSFSSAFFLRYFAEGNLRKGVS
                     QSCGMNNESLTAALACKLSQADLDFLSSLTNFKCFVSASNMKNAAGQYIEAAYAKALR
                     QELASLVQIDKMKGVLSKLEAFAETATPSLDIGDVIVLLGQHPHGSILDINVGTERKT
                     VSVQETRSLGGSKFSVCTVVSNTPVDALTGIPLQTPTPLFENGPRHRSEEDDLKVERM
                     KKHCVSLGFHNINGKVYCKIWDKSTGDTFYTDDSRYTQDHAFQDRSADYRDRDYEGVQ
                     TTPQQGFDPKSETPVGTVVIGGITYNRYLIKGKEVLVPKPDNCLEAAKLSLEQALAGM
                     GQTCDLTAAEVEKLKRIISQLQGLTTEQALNC"
     CDS             12394..13191
                     /standard_name="ORF3"
                     /product="ORF3"
                     /codon_start=1
                     /translation="MAHQCARFHFFLCGFICYLVHSALASNSSSTLCFWFPLAHGNTS
                     FELTINYTICMPCSTSQAARQRLEPGRNMWCKIGHDRCEERDHDELLMSIPSGYDNLK
                     LEGYYAWLAFLSFSYAAQFHPELFGIGNVSRVFVDKRHQFICAEHDGHNSTVSTGHNI
                     SALYAAYYHHQIDGGNWFHLEWLRPLFSSWLVLNISWFLRRSPVSPVSRRIYQILRPT
                     RPRLPVSWSFRTSIVSDLTGSQQRKRKFPSESRPNVVKPSVLPSTSR"
     CDS             12936..13487
                     /standard_name="ORF4"
                     /product="ORF4"
                     /codon_start=1
                     /translation="MAAATLFFLAGAQHIMVSEAFACKPCFSTHLSDIETNTTAAAGF
                     MVLQDINCFRPHGVSAAQEKISFGKSSQCREAVGTPQYITITANVTDESYLYNADLLM
                     LSACLFYASEMSEKGFKVIFGNVSGVVSACVNFTDYVAHVTQHTQQHHLVIDHIRLLH
                     FLTPSAMRWATTIACLFAILLAI"
     CDS             13484..14089
                     /standard_name="ORF5"
                     /product="ORF5"
                     /codon_start=1
                     /translation="MRCSHKLGRFLTPHSCFWWLFLLCTGLSWSFADGNGDSSTYQYI
                     YNLTICELNGTDWLSSHFGWAVETFVLYPVATHILSLGFLTTSHFFDALGLGAVSTAG
                     FVGGRYVLCSVYGACAFAAFVCFVIRAAKNCMACRYARTRFTNFIVDDRGRVHRWKSP
                     IVVEKLGKAEVDGNLVTIKHVVLEGVKAQPLTRTSAEQWEA"
     CDS             14077..14598
                     /standard_name="ORF6"
                     /product="envelope protein"
                     /codon_start=1
                     /translation="MGGLDDFCNDPIAAQKLVLAFSITYTPIMIYALKVSRGRLLGLL
                     HILIFLNCSFTFGYMTYVHFQSTNRVALTLGAVVALLWGVYSFTESWKFITSRCRLCC
                     LGRRYILAPAHHVESAAGLHSISASGNRAYAVRKPGLTSVNGTLVPGLRSLVLGGKRA
                     VKRGVVNLVKYGR"
     CDS             14588..14974
                     /standard_name="ORF7"
                     /product="nucleocapsid protein"
                     /codon_start=1
                     /translation="MAGKNQSQKKKKSTAPMGNGQPVNQLCQLLGAMIKSQRQQPRGG
                     QAKKKKPEKPHFPLAAEDDIRHHLTQTERSLCLQSIQTAFNQGAGTASLSSSGKVSFQ
                     VEFMLPVAHTVRLIRVTSTSASQGAS"
BASE COUNT     3244 a   4137 c   3812 g   3908 t
ORIGIN      
        1 gggtattccc cctacataca cgacacttct agtgtttgtg taccttggag gcgtgggtac
       61 agccccgccc caccccttgg cccctgttct agcccaacag gtatccttct ctctcggggc
      121 gagtgcgccg cctgctgctc ccttgcagcg ggaaggacct cccgagtatt tccggagagc
      181 acctgcttta cgggatctcc accctttaac catgtctggg acgttctccc ggtgcatgtg
      241 caccccggct gcccgggtat tttggaacgc cggccaagtc ttttgcacac ggtgtctcag
      301 tgcgcggtct cttctctctc cagagcttca ggacactgac ctcggtgcag ttggcttgtt
      361 ttacaagcct agggacaagc ttcactggaa agtccctatc ggcatccctc aggtggaatg
      421 tactccatcc gggtgctgtt ggctctcagc tgttttccct ttggcgcgta tgacctccgg
      481 caatcacaac ttcctccaac gacttgtgaa ggttgctgat gttttgtacc gtgacggttg
      541 cttggcacct cgacaccttc gtgaactcca agtttacgag cgcggctgca actggtaccc
      601 gatcacgggg cccgtgcccg ggatgggttt gtttgcgaac tccatgcacg tatccgacca
      661 gccgttccct ggtgccaccc atgtgttgac taactcgcct ttgcctcaac aggcttgtcg
      721 gcagccgttc tgtccatttg aggaggctca ttctagcgtg tacaggtgga agaaatttgt
      781 ggttttcacg gactcctccc tcaacggtcg atctcgcatg atgtggacgc cggaatccga
      841 tgattcagcc gccctggagg tactaccgcc tgagttagaa cgtcaggtcg aaatcctcat
      901 tcggagtttt cctgctcatc accctgtcga cctggccgac tgggagctca ctgagtcccc
      961 tgagaacggt ttttccttca acacgtctca ttcttgcggt caccttgtcc agaaccccga
     1021 cgtgtttgat ggcaagtgct ggctctcctg ctttttgggc cagtcggtcg aagtgcgctg
     1081 ccatgaggaa catctagctg acgccttcgg ttaccaaacc aagtggggcg tgcatggtaa
     1141 gtacctccag cgcaggcttc aagttcgcgg cattcgtgct gtagtcgatc ctgatggtcc
     1201 cattcacgtt gaagcgctgt cttgccccca gtcttggatc aggcacctga ctctggatga
     1261 tgatgtcacc ccaggattcg ttcgcctgac atcccttcgc attgtgccga acacagagcc
     1321 taccacttcc cggatctttc ggtttggagc gcataagtgg tatggcgctg ccggcaaacg
     1381 ggctcgtgct aagcgtgccg ctaaaagtga gaaggattcg gctcccaccc ccaaggttgc
     1441 cctgccggtc cccacctgtg gaattaccac ctactctcca ccgacagacg ggtcttgtgg
     1501 ttggcatgtc cttgccgcca taatgaaccg gatgataaat ggtgacttca cgtcccctct
     1561 gactcagtac aacagaccag aggatgattg ggcttctgat tatgatcttg ttcaggcgat
     1621 tcaatgtcta cgactgcctg ctaccgtggt tcggaatcgc gcctgtccta acgccaagta
     1681 ccttataaaa cttaacggag ttcactggga ggtagaggtg aggtctggaa tggctcctcg
     1741 ctccctttct cgtgaatgtg tggttggcgt ttgctctgaa ggctgtgtcg caccgcctta
     1801 tccagcagac gggctaccta aacgtgcact cgaggccttg gcgtctgctt acagactacc
     1861 ctccgattgt gttagctctg gtattgctga ctttcttgct aatccacctc ctcaggaatt
     1921 ctggaccctc gacaaaatgt tgacctcccc gtcaccagag cggtccggct tctctagttt
     1981 gtataaatta ctattagagg ttgttccgca aaaatgcggt gccacggaag gggctttcat
     2041 ctatgctgtt gagaggatgt tgaaggattg tccgagctcc aaacaggcca tggcccttct
     2101 ggcaaaaatt aaagttccat cctcaaaggc cccgtctgtg tccctggacg agtgtttccc
     2161 tacggatgtt ttagccgact tcgagccagc atctcaggaa aggccccaaa gttccggcgc
     2221 tgctgttgtc ctgtgttcac cggatgcaaa agagttcgag gaagcagccc cggaagaagt
     2281 tcaagagagt ggccacaagg ccgtccactc tgcactcctt gccgagggtc ctaacaatga
     2341 gcaggtacag gtggttgccg gtgagcaact gaagctcggc ggttgtggtt tggcagtcgg
     2401 gaatgctcat gaaggtgctc tggtctcagc tggtctaatt aacctggtag gcgggaattt
     2461 gtccccctca gaccccatga aagaaaacat gctcaatagc cgggaagacg aaccactgga
     2521 tttgtcccaa ccagcaccag cttccacaac gacccttgtg agagagcaaa cacccgacaa
     2581 cccaggttct gatgccggtg ccctccccgt caccgttcga gaatttgtcc cgacggggcc
     2641 tatactctgt catgttgagc actgcggcac ggagtcgggc gacagcagtt cgcctttgga
     2701 tctatctgat gcgcaaaccc tggaccagcc tttaaatcta tccctggccg cttggccagt
     2761 gagggccacc gcgtctgacc ctggctgggt ccacggtagg cgcgagcctg tctttgtaaa
     2821 gcctcgaaat gctttctctg atggcgattc agcccttcag ttcggggagc tttctgaatc
     2881 cagctctgtc atcgagtttg accggacaaa agatgctccg gtggttgacg cccctgtcga
     2941 cttgacgact tcgaacgagg ccctctctgt agtcgatcct ttcgaatttg ccgaactcaa
     3001 gcgcccgcgt ttctccgcac aagccttaat tgaccgaggc ggtccacttg ccgatgtcca
     3061 tgcaaaaata aagaaccggg tatatgaaca gtgcctccaa gcttgtgagc ccggtagtcg
     3121 tgcaacccca gccaccaggg agtggctcga caaaatgtgg gatagggtgg acatgaaaac
     3181 ttggcgctgc acctcgcagt tccaagctgg tcgcattctt gcgtccctca aattcctccc
     3241 tgacatgatt caagacacac cgcctcctgt tcccaggaag aaccgagcta gtgacaatgc
     3301 cggcctgaag caactggtgg cacagtggga taggaaattg agtgtgaccc cccccccaaa
     3361 accggttggg ccagtgcttg accagatcgt ccctccgcct acggatatcc agcaagaaga
     3421 tgtcaccccc tccgatgggc caccccatgc gccggatttt cctagtcgag tgagcacggg
     3481 cgggagttgg aaaggcctta tgctttccgg cacccgtctc gcggggtcta tcagccagcg
     3541 ccttatgaca tgggtttttg aagttttctc ccacctccca gcttttatgc tcacactttt
     3601 ctcgccgcgg ggctctatgg ctccaggtga ttggttgttt gcaggtgtcg ttttacttgc
     3661 tctcttgctc tgtcgttctt acccgatact cggatgcctt cccttattgg gtgtcttttc
     3721 tggttctttg cggcgtgttc gtctgggtgt ttttggttct tggatggctt ttgctgtatt
     3781 tttattctcg actccatcca acccagtcgg ttcttcttgt gaccacgatt cgccggagtg
     3841 tcatgctgag cttttggctc ttgagcagcg ccaactttgg gaacctgtgc gcggccttgt
     3901 ggtcggcccc tcaggcctct tatgtgtcat tcttggcaag ttactcggtg ggtcacgtta
     3961 tctctggcat gttctcctac gtttatgcat gcttgcagat ttggcccttt ctcttgttta
     4021 tgtggtgtcc caggggcgtt gtcacaagtg ttggggaaag tgtataagga cagctcctgc
     4081 ggaggtggct cttaatgtat ttcctttctc gcgcgccacc cgtgtctctc ttgtatcctt
     4141 gtgtgatcga ttccaaacgc caaaaggggt tgatcctgtg cacttggcaa cgggttggcg
     4201 cgggtgctgg cgtggtgaga gccccatcca tcaaccacac caaaagccca tagcttatgc
     4261 caatttggat gaaaagaaaa tgtctgccca aacggtggtt gctgtcccat acgatcccag
     4321 tcaggctatc aaatgcctga aagttctgca ggcgggaggg gccatcgtgg accagcctac
     4381 acctgaggtc gttcgtgtgt ccgagatccc cttctcagcc ccatttttcc caaaagttcc
     4441 agtcaaccca gattgcaggg ttgtggtaga ttcggacact tttgtggctg cggttcgctg
     4501 cggttactcg acagcacaac tggttctggg ccggggcaac tttgccaagt taaatcagac
     4561 cccccccagg aactctatct ccaccaaaac gactggtggg gcctcttaca cccttgctgt
     4621 ggctcaagtg tctgcgtgga ctcttgttca tttcatcctc ggtctttggt tcacatcacc
     4681 tcaagtgtgt ggccgaggaa ccgctgaccc atggtgttca aatccttttt catatcctac
     4741 ctatggcccc ggagttgtgt gctcctctcg actttgtgtg tctgccgacg gggtcaccct
     4801 gccattgttc tcagccgtgg cacaactctc cggtagagag gtggggattt ttattttggt
     4861 gctcgtctcc ttgactgctt tggcccaccg catggctctt aaggcagaca tgttagtggt
     4921 cttttcggct ttttgtgctt acgcctggcc catgagctcc tggttaatct gcttctttcc
     4981 tatactcttg aagtgggtta cccttcaccc tcttactatg ctttgggtgc actcattctt
     5041 ggtgttttgt ctgccagcag ccggcatcct ctcactaggg ataactggcc ttctttgggc
     5101 aattggccgc tttacccagg ttgccggaat tattacacct tatgacatcc accagtacac
     5161 ctctgggcca cgtggtgcag ctgctgtggc cacagcccca gaaggcactt atatggccgc
     5221 cgtccggaga gctgctttaa ctgggcgaac tttaatcttc accccgtctg cagttggatc
     5281 ccttctcgaa ggtgctttca ggactcataa accctgcctt aacaccgtga atgttgtagg
     5341 ctcttccctt ggttccggag gggttttcac cattgatggc agaagaactg tcgtcactgc
     5401 tgcccatgtg ttgaacggcg acacagctag agtcaccggc gactcctaca accgcatgca
     5461 cactttcaag accaatggtg attatgcctg gtcccatgct gatgactggc agggcgttgc
     5521 ccctgtggtc aaggttgcga aggggtaccg cggtcgtgcc tactggcaaa catcaactgg
     5581 tgtcgaaccc ggtatcattg gggaagggtt cgccttctgt tttactaact gcggcgattc
     5641 ggggtcaccc gtcatctcag aatctggtga tcttattgga atccacaccg gttcaaacaa
     5701 acttggttct ggtcttgtga caacccctga aggggagacc tgcaccatca aagaaaccaa
     5761 gctctctgac ctttccagac attttgcagg cccaagcgtt cctcttgggg acattaaatt
     5821 gagtccggcc atcatccctg atgtaacatc cattccgagt gacttggcat cgctcctagc
     5881 ctccgtccct gtagtggaag gcggcctctc gaccgttcaa cttttgtgtg tctttttcct
     5941 tctctggcgc atgatgggcc atgcctggac acccattgtt gccgtgggct tctttttgct
     6001 gaatgaaatt cttccagcag ttttggtccg agccgtgttt tcttttgcac tctttgtgct
     6061 tgcatgggcc accccctggt ctgcacaggt gttgatgatt agactcctca cggcatctct
     6121 caaccgcaac aagctttctc tggcgttcta cgcactcggg ggtgtcgtcg gtttggcagc
     6181 tgaaatcggg acttttgctg gcagattgtc tgaattgtct caagctcttt cgacatactg
     6241 cttcttacct agggtccttg ctatgaccag ttgtgttccc accatcatca ttggtggact
     6301 ccataccctc ggtgtgattc tgtggttatt caaataccgg tgcctccaca acatgctggt
     6361 tggtgatggg agtttttcaa gcgccttctt cctacggtat tttgcagagg gtaatctcag
     6421 aaaaggtgtt tcacagtcct gtggcatgaa taacgagtcc ctaacggctg ctttagcttg
     6481 caagttgtca caggctgacc ttgatttttt gtccagctta acgaacttca agtgctttgt
     6541 atctgcttca aacatgaaaa atgctgccgg ccagtacatt gaagcagcgt atgccaaggc
     6601 cctgcgccaa gagttggcct ctctagttca gattgacaaa atgaaaggag ttttgtccaa
     6661 gctcgaggcc tttgctgaaa cagccacccc gtcccttgac ataggtgacg tgattgttct
     6721 gcttgggcaa catcctcacg gatccatcct cgatattaat gtggggactg aaaggaaaac
     6781 tgtgtccgtg caagagaccc ggagcctagg cggctccaaa ttcagtgttt gtactgtcgt
     6841 gtccaacaca cccgtggacg ccttgaccgg catcccactc cagacaccaa cccctctttt
     6901 tgagaatggt ccgcgtcatc gcagcgagga agacgatctt aaagtcgaga ggatgaagaa
     6961 acactgtgta tccctcggct tccacaacat caatggcaaa gtttactgca aaatttggga
     7021 caagtctacc ggtgacacct tttacacgga tgattcccgg tacacccaag accatgcttt
     7081 tcaggacagg tcagccgact acagagacag ggactatgag ggtgtgcaaa ccacccccca
     7141 acagggattt gatccaaagt ctgaaacccc tgttggcact gttgtgatcg gcggtattac
     7201 gtataacagg tatctgatca aaggtaagga ggttctggtc cccaagcctg acaactgcct
     7261 tgaagctgcc aagctgtccc ttgagcaagc tctcgctggg atgggccaaa cttgcgacct
     7321 tacagctgcc gaggtggaaa agctaaagcg catcattagt caactccaag gtttgaccac
     7381 tgaacaggct ttaaactgtt agccgccagc ggcttgaccc gctgtggccg cggcggccta
     7441 gttgtgactg aaacggcggt aaaaattata aaataccaca gcagaacttt caccttaggc
     7501 cctttagacc taaaagtcac ttccgaggtg gaggtaaaga aatcaactga gcagggccac
     7561 gctgttgtgg caaacttatg ttccggtgtc atcttgatga gacctcaccc accgtccctt
     7621 gtcgacgttc ttctgaaacc cggacttgac acaatacccg gcattcaacc agggcatggg
     7681 gccgggaata tgggcgtgga cggttctatt tgggattttg aaaccgcacc cacaaaggca
     7741 gaactcgagt tatccaagca aataatccaa gcatgtgaag ttaggcgcgg ggacgccccg
     7801 aacctccaac tcccttacaa gctctatcct gttagggggg atcctgagcg gcataaaggc
     7861 cgccttatca ataccaggtt tggagattta ccttacaaaa ctcctcaaga caccaagtcc
     7921 gcaatccacg cggcttgttg cctgcacccc aacggggccc ccgtgtctga tggtaaatcc
     7981 acactaggta ccactcttca acatggtttc gagctttatg tccctactgt gccctatagt
     8041 gtcatggagt accttgattc acgccctgac acccctttta tgtgtactaa acatggcact
     8101 tccaaggctg ctgcagagga cctccaaaaa tacgacctat ccacccaagg atttgtcctg
     8161 cctggggtcc tacgcctagt acgcagattc atctttggcc atattggtaa ggcgccgcca
     8221 ttgttcctcc catcaaccta tcccgccaag aactctatgg cagggatcaa tggccagagg
     8281 ttcccaacaa aggacgttca gagcatacct gaaattgatg aaatgtgtgc ccgcgctgtc
     8341 aaggagaatt ggcaaactgt gacaccttgc accctcaaga aacagtactg ttccaagccc
     8401 aaaaccagga ccatcctggg caccaacaac tttattgcct tggctcacag atcggcgctc
     8461 agtggtgtca cccaggcatt catgaagaag gcttggaagt ccccaattgc cttggggaaa
     8521 aacaaattca aggagctgca ttgcactgtc gccggcaggt gtcttgaggc cgacttggcc
     8581 tcctgtgacc gcagcacccc cgccattgta agatggtttg ttgccaacct cctgtatgaa
     8641 cttgcaggat gtgaagagta cttgcctagc tatgtgctta attgctgcca tgacctcgtg
     8701 gcaacacagg atggtgcctt cacaaaacgc ggtggcctgt cgtccgggga ccccgtcacc
     8761 agtgtgtcca acaccgtata ttcactggta atttatgccc agcacatggt attgtcggcc
     8821 ttgaaaatgg gtcatgaaat tggtcttaag ttcctcgagg aacagctcaa gttcgaggac
     8881 ctccttgaaa ttcagcctat gttggtatac tctgatgatc ttgtcttgta cgctgaaaga
     8941 cccacatttc ccaattacca ctggtgggtc gagcaccttg acctgatgct gggtttcaga
     9001 acggacccaa agaaaaccgt cataactgat aaacccagct tcctcggctg cagaattgag
     9061 gcagggcgac agctagtccc caatcgcgac cgcatcctgg ctgctcttgc atatcacatg
     9121 aaggcgcaga acgcctcaga gtattatgcg tctgctgccg caatcctgat ggattcatgt
     9181 gcttgcattg accatgaccc tgagtggtat gaggacctca tctgcggtat tgcccggtgc
     9241 gcccgccagg atggttatag cttcccaggt ccggcatttt tcatgtccat gtgggagaag
     9301 ctgagaagtc ataatgaagg gaagaaattc cgccactgcg gcatctgcga cgccaaagcc
     9361 gactatgcgt ccgcctgtgg gcttgatttg tgtttgttcc attcgcactt tcatcaacac
     9421 tgccctgtca ctctgagctg cggtcaccat gccggttcaa aggaatgttc gcagtgtcag
     9481 tcacctgttg gggctggcag atcccctctt gatgccgtgc taaaacaaat tccatacaaa
     9541 cctcctcgta ctgtcatcat gaaggtgggt aataaaacaa cggccctcga tccggggagg
     9601 taccagtccc gtcgaggtct cgttgcagtc aagaggggta ttgcaggcaa tgaagttgat
     9661 ctttctgatg gggactacca agtggtgcct cttttgccga cttgcaaaga cataaacatg
     9721 gtgaaggtgg cttgcaatgt actactcagc aagttcatag tagggccacc aggttccgga
     9781 aagaccacct ggctactgag tcaagtccag gacgatgatg tcatttacac acccacccat
     9841 cagactatgt ttgatatagt cagtgctctc aaagtttgca ggtattccat tccaggagcc
     9901 tcaggactcc ctttcccacc acctgccagg tccgggccgt gggttaggct tattgccagc
     9961 gggcacgtcc ctggccgagt atcatacctc gatgaggctg gatattgtaa tcatctggac
    10021 attcttagac tgctttccaa aacacccctt gtgtgtttgg gtgaccttca gcaacttcac
    10081 cctgtcggct ttgattccta ctgttatgtg ttcgatcaga tgcctcagaa gcagctgacc
    10141 actatttaca gatttggccc taacatctgc gcacgcatcc agccttgtta cagggagaaa
    10201 cttgaatcta aggctaggaa cactagggtg gtttttacca cccggcctgt ggcctttggt
    10261 caggtgctga caccatacca taaagatcgc atcggctctg cgataaccat agattcatcc
    10321 cagggggcca cctttgatat tgtgacattg catctaccat cgccaaagtc cctaaataaa
    10381 tcccgagcac ttgtagccat cactcgggca agacacgggt tgttcattta tgaccctcat
    10441 aaccagctcc aggagttttt caacttaacc cctgagcgca ctgattgtaa ccttgtgttc
    10501 agccgtgggg atgagctggt agttctgaat gcggataatg cagtcacaac tgtagcgaag
    10561 gcccttgaga caggtccatc tcgatttcga gtatcagacc cgaggtgcaa gtctctctta
    10621 gccgcttgtt cggccagtct ggaagggagc tgtatgccac taccgcaagt ggcacataac
    10681 ctggggtttt acttttcccc ggacagtcca acatttgcac ctctgccaaa agagttggcg
    10741 ccacattggc cagtggttac ccaccagaat aatcgggcgt ggcctgatcg acttgtcgct
    10801 agtatgcgcc caattgatgc ccgctacagc aagccaatgg tcggtgcagg gtatgtggtc
    10861 gggccgtcca cctttcttgg tactcctggt gtggtgtcat actatctcac actatacatc
    10921 aggggtgagc cccaggcctt gccagaaaca ctcgtttcaa cagggcgtat agccacagat
    10981 tgtcgggagt atctcgacgc ggctgaggaa gaggcagcaa aagaactccc ccacgcattc
    11041 attggcgatg tcaaaggtac cacggttggg gggtgtcatc acattacatc aaaataccta
    11101 cctaggtccc tgcctaagga ctctgttgcc gtagttggag taagttcgcc cggcagggct
    11161 gctaaagccg tgtgcactct caccgatgtg tacctccccg aactccggcc atatctgcaa
    11221 cctgagacgg catcaaaatg ctggaaactc aaattagact tcagggacgt ccgactaatg
    11281 gtctggaaag gagccaccgc ctatttccag ttggaagggc ttacatggtc ggcgctgccc
    11341 gactatgcca ggtttattca gctgcccaag gatgccgttg tatacattga tccgtgtata
    11401 ggaccggcaa cagccaaccg taaggtcgtg cgaaccacag actggcgggc cgacctggca
    11461 gtgacaccgt atgattacgg tgcccagaac attttgacaa cagcctggtt cgaggacctc
    11521 gggccgcagt ggaagatttt ggggttgcag ccctttaggc gagcatttgg ctttgaaaac
    11581 actgaggatt gggcaatcct tgcacgccgt atgaatgacg gcaaggacta cactgactat
    11641 aactggaact gtgttcgaga acgcccacac gccatctacg ggcgtgctcg tgaccatacg
    11701 tatcattttg cccctggcac agaattgcag gtagagctag gtaaaccccg gctgccgcct
    11761 gggcaagtgc cgtgaattcg gggtgatgca atggggtcac tgtggagtaa aatcagccag
    11821 ctgttcgtgg acgccttcac tgagttcctt gttagtgtgg ttgatattgc cattttcctt
    11881 gccatactgt ttgggttcac cgtcgcagga tggttactgg tctttcttct cagagtggtt
    11941 tgctccgcgc ttctccgttc gcgctctgcc attcactctc ccgaactatc gaaggtccta
    12001 tgaaggcttg ttgcccaact gcagaccgga tgtcccacaa tttgcagtca agcacccatt
    12061 gggtatgttt tggcacatgc gagtttccca cttgattgat gagatggtct ctcgtcgcat
    12121 ttaccagacc atggaacatt caggtcaagc ggcctggaag caggtggttg gtgaggccac
    12181 tctcacgaag ctgtcagggc tcgatatagt tactcatttc caacacctgg ccgcagtgga
    12241 ggcggattct tgccgctttc tcagctcacg actcgtgatg ctaaaaaatc ttgccgttgg
    12301 caatgtgagc ctacagtaca acaccacgtt ggaccgcgtt gagctcatct tccccacgcc
    12361 aggtacgagg cccaagttga ccgatttcag acaatggctc atcagtgtgc acgcttccat
    12421 tttttcctct gtggcttcat ctgttacctt gttcatagtg ctttggcttc gaattccagc
    12481 tctacgctat gtttttggtt tccattggcc cacggcaaca catcattcga gctgaccatc
    12541 aactacacca tatgcatgcc ctgttctacc agtcaagcgg ctcgccaaag gctcgagccc
    12601 ggtcgtaaca tgtggtgcaa aatagggcat gacaggtgtg aggagcgtga ccatgatgag
    12661 ttgttaatgt ccatcccgtc cgggtacgac aacctcaaac ttgagggtta ttatgcttgg
    12721 ctggcttttt tgtccttttc ctacgcggcc caattccatc cggagttgtt cgggataggg
    12781 aatgtgtcgc gcgtcttcgt ggacaagcga caccagttca tttgtgccga gcatgatgga
    12841 cacaattcaa ccgtatctac cggacacaac atctccgcat tatatgcggc atattaccac
    12901 caccaaatag acgggggcaa ttggttccat ttggaatggc tgcggccact cttttcttcc
    12961 tggctggtgc tcaacatatc atggtttctg aggcgttcgc ctgtaagccc tgtttctcga
    13021 cgcatctatc agatattgag accaacacga ccgcggctgc cggtttcatg gtccttcagg
    13081 acatcaattg tttccgacct cacggggtct cagcagcgca agagaaaatt tccttcggaa
    13141 agtcgtccca atgtcgtgaa gccgtcggta ctccccagta catcacgata acggctaacg
    13201 tgaccgacga atcatacttg tacaacgcgg acctgctgat gctttctgcg tgccttttct
    13261 acgcctcaga aatgagcgag aaaggcttca aagtcatctt tgggaatgtc tctggcgttg
    13321 tttctgcttg tgtcaatttc acagattatg tggcccatgt gacccaacat acccagcagc
    13381 atcatctggt aattgatcac attcggttgc tgcatttcct gacaccatct gcaatgaggt
    13441 gggctacaac cattgcttgt ttgttcgcca ttctcttggc aatatgagat gttctcacaa
    13501 attggggcgt ttcttgactc cgcactcttg cttctggtgg ctttttttgc tgtgtaccgg
    13561 cttgtcctgg tcctttgccg atggcaacgg cgacagctcg acataccaat acatatataa
    13621 cttgacgata tgcgagctga atgggaccga ctggttgtcc agccattttg gttgggcagt
    13681 cgagaccttt gtgctttacc cggttgccac tcatatcctc tcactgggtt ttctcacaac
    13741 aagccatttt tttgacgcgc tcggtctcgg cgctgtatcc actgcaggat ttgttggcgg
    13801 gcggtacgta ctctgcagcg tctacggcgc ttgtgctttc gcagcgttcg tatgttttgt
    13861 catccgtgct gctaaaaatt gcatggcctg ccgctatgcc cgtacccggt ttaccaactt
    13921 cattgtggac gaccggggga gagttcatcg atggaagtct ccaatagtgg tagaaaaatt
    13981 gggcaaagcc gaagtcgatg gcaacctcgt caccatcaaa catgtcgtcc tcgaaggggt
    14041 taaagctcaa cccttgacga ggacttcggc tgagcaatgg gaggcctaga cgatttttgc
    14101 aacgatccta tcgccgcaca aaagctcgtg ctagccttta gcatcacata cacacctata
    14161 atgatatacg cccttaaggt gtcacgcggc cgactcctgg ggctgttgca catcctaata
    14221 tttctgaact gttcctttac attcggatac atgacatatg tgcattttca atccaccaac
    14281 cgtgtcgcac ttaccctggg ggctgttgtc gcccttctgt ggggtgttta cagcttcaca
    14341 gagtcatgga agtttatcac ttccagatgc agattgtgtt gccttggccg gcgatacatt
    14401 ctggcccctg cccatcacgt agaaagtgct gcaggtctcc attcaatctc agcgtctggt
    14461 aaccgagcat acgctgtgag aaagcccgga ctaacatcag tgaacggcac tctagtacca
    14521 ggacttcgga gcctcgtgct gggcggcaaa cgagctgtta aacgaggagt ggttaacctc
    14581 gtcaagtatg gccggtaaaa accagagcca gaagaaaaag aaaagtacag ctccgatggg
    14641 gaatggccag ccagtcaatc aactgtgcca gttgctgggt gcaatgataa agtcccagcg
    14701 ccagcaacct aggggaggac aggccaaaaa gaaaaagcct gagaagccac attttcccct
    14761 ggctgctgaa gatgacatcc ggcaccacct cacccagact gaacgctccc tctgcttgca
    14821 atcgatccag acggctttca atcaaggcgc aggaactgcg tcgctttcat ccagcgggaa
    14881 ggtcagtttt caggttgagt ttatgctgcc ggttgctcat acagtgcgcc tgattcgcgt
    14941 gacttctaca tccgccagtc agggtgcaag ttaatttgac agtcaggtga atggccgcga
    15001 ttggcgtgtg gcctctgagt cacctattca attagggcga tcacatgggg gtcatactta
    15061 atcaggcagg aaccatgtga ccgaaattaa aaaaaaaaaa a
//