From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Coturnix coturnix (alternatively spliced) serine/threonine protein
Date: 24 Jul 92 10:09:15 GMT
Distribution: bionet
Checksum: 05918     7

LOCUS       QULCRMILA    2547 bp ss-mRNA            VRT       24-JUL-1992
DEFINITION  Coturnix coturnix (alternatively spliced) serine/threonine protein
            kinase (c-Rmil) mRNA, complete cds.
ACCESSION   M80846
KEYWORDS    B-raf oncogene; alternative splicing; c-Rmil protein;
            proto-oncogene; raf family; serine/threonine protein kinase.
SOURCE      Coturnix coturnix (sub_species japonica) (library: lambda gt-10)
            13-day old embryo cDNA to mRNA.
  ORGANISM  Coturnix coturnix
            Eukaryota; Animalia; Chordata; Vertebrata; Aves; Neornithes;
            Neognathae; Galliformes; Phasianidae.
REFERENCE   1  (bases 1 to 2547)
  AUTHORS   Eychene,A., Barnier,J.V., Dezelee,P., Marx,M., Laugier,D.,
            Calogeraki,I. and Calothy,G.
  TITLE     Quail neuroretina c-Rmil(B-raf) protooncogene cDNAs encode two
            proteins of 93.5 and 95 KD resulting from alternative splicing
  JOURNAL   Oncogene 7, 1315-1323 (1992)
  STANDARD  full staff_entry
FEATURES             Location/Qualifiers
     CDS             1..2424
                     /note="95 kilodalton protein which is homologous to the
                     avian transduced v-Rmil and the mammalian B-raf genes"
                     /product="serine/threonine protein kinase"
                     /gene="c-Rmil"
                     /note="putative"
                     /codon_start=1
                     /translation="MAALSSGSSAEGASLFNGDMEPEPPPPVLGACYAGSGGGDPAIP
                     EEVWNIKQMIKLTQEHIEALLDKFGGEHNPPSIYLEAYEEYTSKLDALQQREQQLLES
                     MGNGTDFSVSSSASTDTVASSSSSSLSVAPSSLSVYQNPTDMSRNNPKSPQKPIVRVF
                     LPNKQRTVVPARCGVTVRDSLKKALMMRGLIPECCAVYRIQDGEKKPIGWDTDISWLT
                     GEELHVEVLENVPLTTHNFVRKTFFTLAFCDFCRKLLFQGFRCQTCGYKFHQRCSTEV
                     PLMCVNYDQLDLLFVSKFFEHHPISQEETTLGETTPASGSYPSVPPSDSVGPPILPSP
                     SPSKSIPIPQPFRPADEDHRNQFGQRDRSSSAPNVHINTIEPVNIDDLIRDQGVRGEG
                     APLNQLMRCLRKYQSRTPSPLLHSVPSEIVFDFEPGPVFRGSTAGLSATPPASLPGSL
                     TNVKALQKSPGPQRERKSSSSSEDRNRMKTLGRRDSSDDWEIPDGQITVGQRIGSGSF
                     GTVYKGKWHGDVAVKMLNVTAPTPQQLQAFKNEVGVLRKTRHVNILLFMGYSTKPQLA
                     IVTQWCEGSSLYHHLHIIETKFEMIKLIDIARQTAQGMDYLHAKSIIHRDLKSNNIFL
                     HEDLTVKIGDFGLATVKSRWSGSHQFEQLSGSILWMAPEVIRMQDKNPYSFQSDVYAF
                     GIVLYELMTGQLPYSNINNRDQIIFMVGRGYLSPDLSKVRSNCPKAMKRLMAECLKKK
                     RDERPLFPQILASIELLARSLPKIHRSASEPSLNRAGFQTEDFSLYACASPKTPIQAG
                     GYGEFAAFK"
     exon            1178..1298
                     /note="bases 1178-1298 correspond to an alternatively
                     spliced exon"
                     /gene="c-Rmil"
     misc_feature    1567..1569
                     /note="base pairs 1567 through 1569 encodes for the lysine
                     residue responsible for the ATP-binding activity of the
                     serine/threonine protein kinase"
                     /gene="c-Rmil"
     3'UTR           2425..2547
                     /gene="c-Rmil"
BASE COUNT      730 a    619 c    580 g    618 t
ORIGIN
        1 atggcggcgc tgagcagcgg cagcagcgcc gagggggcct cgctcttcaa cggggacatg
       61 gagcccgagc cgccgccgcc cgtgctgggc gcctgctacg ccgggagcgg cggcggcgac
      121 ccggccatcc cggaggaggt gtggaatatc aaacagatga ttaaattaac acaagaacat
      181 atagaagcgc tgttagacaa gtttggagga gaacataacc caccgtcaat atatctagag
      241 gcctatgaag agtacaccag caaacttgat gctctacagc agagagagca gcagttattg
      301 gaatccatgg gaaatggaac tgatttctct gtctccagtt cagcttcaac ggacacagtt
      361 gcatcatctt cctcctctag cctctctgta gcaccttcat ccctttcagt ttatcaaaat
      421 cctactgata tgtcacggaa taaccctaag tctccacaga agcctattgt tagagtcttc
      481 ctgcccaaca agcaaagaac tgtggttccg gcaagatgtg gggtaacagt ccgagacagc
      541 ctcaagaaag ctctgatgat gagaggtctt attccagaat gctgcgctgt ttacagaata
      601 caggatggag agaagaagcc aattggctgg gacactgaca tttcctggct aaccggagag
      661 gagttacatg tggaggtctt ggagaatgtg ccactcacaa cacacaattt tgtacgaaaa
      721 acattcttca cgttagcgtt ctgcgacttc tgtcgaaagc tgcttttcca gggattccga
      781 tgccagacat gtggctacaa atttcaccag cgctgtagca cagaagtgcc actgatgtgt
      841 gttaactacg accaactcga tttgctgttt gtctccaagt tctttgaaca tcaccccata
      901 tcgcaggagg agaccacctt aggagagacc accccagcat caggatcgta cccctcagtg
      961 cccccatcag attctgttgg accaccaatt ctccctagtc cttctccttc aaaatccatt
     1021 ccaatcccac agcccttccg accagcagat gaagaccatc ggaatcagtt tgggcaacgt
     1081 gaccgatcct cttcagctcc caatgttcac atcaatacaa tcgagccagt caatattgat
     1141 gacttgatta gagaccaggg tgtacgagga gagggagccc ctttgaacca gctgatgcgc
     1201 tgtcttcgga aataccaatc ccggactccc agtcccctcc ttcattctgt ccccagtgaa
     1261 atagtgtttg attttgagcc tggcccagtg ttcagaggtt caactgcagg tttgtctgca
     1321 acacctcccg catctttgcc tgggtcactt accaatgtga aagcattaca gaaatcacca
     1381 ggcccccagc gggaaaggaa atcgtcctca tcctcagaag acagaaatcg aatgaaaacc
     1441 cttggtcgac gagattcaag tgatgattgg gaaataccag atgggcagat cacagttgga
     1501 caaaggatag gatctggatc attcggaaca gtctacaaag gaaagtggca tggtgacgtg
     1561 gcagtgaaaa tgttgaatgt tacagcaccc acacctcaac agttacaggc tttcaaaaat
     1621 gaagtaggag tgctcaggaa aacacggcat gtgaatatcc tactttttat gggttattca
     1681 acaaaacctc agttggctat tgttacacag tggtgtgagg ggtccagctt atatcaccat
     1741 ctgcacataa ttgagaccaa gtttgaaatg atcaaactaa ttgatattgc acgacagact
     1801 gcacaaggca tggattattt gcatgccaag tcaatcatcc acagagacct caagagtaat
     1861 aatatttttc ttcatgaaga cctcacagta aaaataggtg actttggtct ggctacagtg
     1921 aaatcacgat ggagtggatc tcatcaattt gaacagttat ctggatcgat cctatggatg
     1981 gcaccggaag tgatcaggat gcaagacaaa aacccatata gctttcagtc agatgtgtat
     2041 gcatttggga ttgtgcttta tgaactgatg actggacagt taccatactc aaacatcaac
     2101 aacagggacc agataatttt tatggtggga cgaggatatc tatctccaga cctcagtaaa
     2161 gtaagaagta actgtccaaa agctatgaag agactaatgg cagaatgctt gaaaaagaaa
     2221 agagatgaga ggcctctttt tccacagatt cttgcctcca ttgagcttct ggcccggtcg
     2281 ttgccaaaaa ttcaccgcag tgcatctgag ccgtcactaa accgggctgg cttccagacc
     2341 gaggatttta gtctatatgc ttgtgcttct ccaaaaacgc ccatccaagc agggggatac
     2401 ggagaatttg cagcgttcaa gtagccacag caccatggca gcacccactc tgttatttaa
     2461 gtcttgtgtt cctaccgttt cttaacatca aaactctatt ctgctccaac aaacctaaag
     2521 taatttagaa aagatatccg taagctt
//