From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: D.pseudoobscura Adh gene for alcohol dehydrogenase
Date: 28 Dec 92 21:26:50 GMT
Distribution: bionet
Checksum: 39816 8

LOCUS       DROADHGN25   3479 bp ds-DNA             INV       01-DEC-1992
DEFINITION  D.pseudoobscura Adh gene for alcohol dehydrogenase
ACCESSION   X62205
KEYWORDS    ADH gene; alcohol dehydrogenase.
SOURCE      Drosophila pseudoobscura DNA.
  ORGANISM  Drosophila pseudoobscura
            Eukaryota; Animalia; Metazoa; Arthropoda; Uniramia; Insecta;
            Pterygota; Neoptera; Holometabola; Diptera; Brachycera;
            Cyclorrhapha; Schizophora; Drosophiloidea; Drosophilidae.
REFERENCE   1  (bases 1 to 3479)
  AUTHORS   Schaeffer,S.W. and Miller,E.L.
  TITLE     Estimates of gene flow in Drosophila pseudoobscura determined from
            nucleotide sequence analysis of the alcohol dehydrogenase region
  JOURNAL   Genetics 132, 471-480 (1992)
  STANDARD  full automatic
COMMENT     *source: strain=GB33; *source: isolate=Gundlach Bundschu Winery,
            Sonoma Valley, *source: California; *source: chromosome=IV section
            88;
               From EMBL    entry DPADHGN25;  dated 16-OCT-1992.
FEATURES             Location/Qualifiers
     mRNA            join(40..119,921..1026,1090..1494,1555..1970)
                     /note="adult mRNA (Adh)"
     mRNA            join(888..1026,1090..1494,1555..1970)
                     /note="larval mRNA (Adh)"
     promoter        8..14
                     /note="adult promoter (Adh)"
     prim_transcript 40..1970
                     /note="adult primary transcript (Adh)"
     exon            40..119
                     /note="adult exon 1a"
     intron          120..920
                     /note="adult intron"
     promoter        852..859
                     /note="larval promoter (Adh)"
     prim_transcript 888..1970
                     /note="larval primary transcript (Adh)"
     exon            888..1026
                     /note="larval exon 1"
     exon            921..1026
                     /note="adult exon 1b"
     intron          1027..1089
                     /number=1
     exon            1090..1494
                     /number=2
     intron          1495..1554
                     /number=2
     exon            1555..1970
                     /number=3
     polyA_signal    1947..1952
     polyA_site      1970
     CDS             join(934..1026,1090..1494,1555..1821)
                     /EC_number="1.1.1.1"
                     /gene="Adh"
                     /product="alcoholdehydrogenase"
                     /codon_start=1
                     /translation="MSLTNKNVVFVAGLGGIGLDTSRELVKRNLKNLVILDRIDNPAA
                     IAELKAINPKVTITFYPYDVTVPVAETTKLLKTIFAQVKTIDVLINGAGILDDHQIER
                     TIAVNYTGLVNTTTAILDFWDKRKGGPGGIICNIGSVTGFNAIYQVPVYSGSKAAVVN
                     FTSSLAKLAPITGVTAYTVNPGITKTTLVHKFNSWLDVEPRVAEKLLEHPTQTSQQCA
                     ENFVKAIELNKNGAIWKLDLGTLEPITWTQHWDSGI"
BASE COUNT      973 a    799 c    795 g    912 t
ORIGIN      
        1 cgtagtgtat ttaaggcact gcacatgtcg aggaccacga ttattgtcta cagtgcagtc
       61 agtagttgtc aggtgcagca ggcggaccag catttcttta aatctactca atcgatcaag
      121 taagtaactg taatccaagt acagtcggcc catcccactc ggtcactcgg ttctgccgta
      181 actcaagaaa tttcttgata gcacagttcc agttgtaaaa cgtcgtctaa aaaaacatct
      241 cgttggagct tgggttgaaa aacgttcgag ttgggcgtag aaaagttcaa atataaaata
      301 tcaaaaaaga gaaaatatct tcccaaagca gctgtccatt ttttgttaaa gtgatagatc
      361 aaaaattcgt atgacgtatt tcccaagtgc tctttatgca aataatttcc tcaattctaa
      421 ttctacaaga agcacttttg gctcttgtcc agaaaaaaca atttgtttac acttttcggt
      481 tgataacaga agcggaccga cggctaagta acataacagt cgcctgttaa gtaatgccct
      541 cactctctgt tagatggccg cgcgtgtcga ctccgataga ccagcagaca cgcagtagtt
      601 ttcctcaaga acctgaactc taaacataga cataatttac gataccttga gactcaaata
      661 aaaatacaag tatgagtggt attctattcc tgattagact cgcacacaca cgtacgagta
      721 ttaacataaa tttgccggca aataaccaaa acaaaacgaa cgcaaacgat ccgaacacgt
      781 aatgcgagag ataagaaaca gaaaagctcg acgtgaaaga agcttctggc ggaagctctt
      841 gccgaactcc ctataaatac gagacagaaa accagaataa atataataac aaactgttga
      901 attgtcccat cgattcccag atcacaagac agaatgtctc tcaccaacaa gaacgtcgtt
      961 ttcgtggccg gtctgggcgg cattggcctg gacaccagcc gggagttggt caagcgtaat
     1021 ctgaaggtaa gagagaacga attccatgga atctatggaa tcctaaattt aaaaaatcat
     1081 ttattttaga acctggtcat cctggatcgc attgacaacc cggctgccat tgccgaactg
     1141 aaggcaatca atcccaaggt gaccatcacc ttctatccct acgatgtgac tgtgcccgtc
     1201 gctgagacca ccaagctcct gaagaccatc tttgcccagg tgaagacaat cgatgtcctg
     1261 atcaacggtg ctggcatcct ggacgatcat cagatcgagc gcaccattgc cgttaactac
     1321 acgggcctgg tcaacaccac cacagccatt ctggacttct gggacaagcg caagggcggc
     1381 ccaggcggca tcatttgcaa cattggctcc gtcaccggtt tcaatgccat ctaccaggtg
     1441 cccgtttact ccggctccaa ggcggcggtg gttaacttta cctcctccct ggcggtaagt
     1501 aatccaacct ttttcctact ccccaaaact aaatatttct ttgaaatccc atagaaactg
     1561 gctcccatta ctggtgtcac tgcttacact gtcaatcctg gcatcactaa gaccactctg
     1621 gtccacaaat tcaactcgtg gctggatgtg gagccccgtg tggcagagaa gctgctcgag
     1681 catcccaccc agacctctca gcagtgcgcc gagaactttg tgaaggccat cgagctgaac
     1741 aagaacggtg ccatctggaa gttggatttg ggcaccttgg agcccatcac atggacccag
     1801 cactgggact cgggcatcta aaccatccca gagactctat gggacatggc gttttagctt
     1861 ttagtttctt tttttccact caattgttac gtatatacat acacatatgg caataaggct
     1921 gatttgaacc ccgttttgaa tatgataata caaaattata tttgagaaat ttcaacaaaa
     1981 tcgataagag ctatagctct cttttaatta aaaaaaacta gataaaaaga gcaatggtca
     2041 gtggtggtca gtggtcgttg tggcctctat cgatttccac acaaaaactt tactgtttag
     2101 taatagaaaa gaacttgaga aaggcagcca aaagaatgta cgatctgacg ggtaagcatg
     2161 tctgctatgt ggctgactgc ggtggcattg cactggagac cagcaaggtt ctcatgacca
     2221 agaatatagc ggtgagtggt agagtggtgt agagagtgga gagtggttct ggttcttaaa
     2281 caagtctcta gtctctagtc tctagtctct tggctgctgc tggtagctgt cgaaactaat
     2341 taagttaatg actttttcat gttttgtttt tgtccgctga cttttgctgt tcaaagctac
     2401 aattaagttt atgttttgag tttgatctac ttttgcttaa gggaagtact ttcgattacg
     2461 gattgctggc atataaaaat agataccaag gaatgtgcct catatcatat tgattctatc
     2521 ttgattcttt gcccagaaac tggccatcct tcagagcgtg gaaaaccccc cggccatcgc
     2581 tcagttacaa tcgattaagc acagcacaca gatcttcttc tggaccttcg atgtgaccat
     2641 ggcccgcgag gagatgaaga agtacttcga tgaggtcatg gtccagatgg actacatcga
     2701 tgtgctgatc aatggggcca ccctgtgcga tgagcggaac attgatgcca ccatcaatac
     2761 gaatctgacc ggcatgatga acaccgtggc cactgtgctg ccctatatgg accgtaagat
     2821 gggcggttcg gggggactga tcgtgaatgt cacctccgtc ataggactgg atccatcgcc
     2881 ggtcttttgt gcgtacagtg cctcaaagtt tggtgtgatt gggttcacca gaagtctagc
     2941 ggtgagtggg ggagatctta acttgaaaat gctctctaat gtctttcatt ctttcgcagg
     3001 atcccctgta ttacacccaa aatggtgtgg ctgtcatggc cgtctgctgt ggtcccacca
     3061 aagtgtttgt cgatcgggaa ctgactgcct tcctggaata cggccaaacc tttgccgatc
     3121 gcctgcgtcg tgccccctgc caatcgacgg ccgtctgcgg ccagaatata gtaaatgcca
     3181 tcgagagatc ggagaacgga cagatctgga ttgccgacaa gggcggcctg gagtcggtgg
     3241 ccctccactg gtattggcat atggccgatc agtttgtgaa ctacatgcag agcactgacg
     3301 atgaggatca ggagtttttt ttgggccagc gatgaggcat gccttgatcc acggaataaa
     3361 acatcaaatg tgtcacaaaa agtaggcaac ttgtttgtgg gtctggaaat agatttgaaa
     3421 tattttgagg tcatatgaat aatgacataa atgagggtgc ctgaaagtat gccaagttt
//