From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Aeromonas hydrophila extracellular deoxyribonuclease (dns) gene,
Date: 20 Aug 92 10:00:44 GMT
Distribution: bionet
Checksum: 06598     4

LOCUS       AEOXDN       1161 bp ds-DNA             BCT       20-AUG-1992
DEFINITION  Aeromonas hydrophila extracellular deoxyribonuclease (dns) gene,
            complete cds.
ACCESSION   M99491
KEYWORDS    deoxyribonuclease; dns gene.
SOURCE      Aeromonas hydrophila (strain CHC-1) DNA.
  ORGANISM  Aeromonas hydrophila
            Prokaryotae; Facultatively anaerobic gram-negative rods;
            Vibrionaceae.
REFERENCE   1  (sites)
  AUTHORS   Focareta,T. and Manning,P.A.
  TITLE     Extracellular proteins of Vibrio cholerae: Molecular cloning,
            nucleotide sequence and characterization of the deoxyribonuclease
            (DNase) together with its periplasmic localization in Escherichia
            coli K-12
  JOURNAL   Gene 53, 31-40 (1987)
  STANDARD  full staff_review
REFERENCE   2  (bases 1 to 1161)
  AUTHORS   Chang,M.-C., Chang,S.-Y., Chen,S.-L. and Chuang,S.-M.
  TITLE     Cloning and expression in Escherichia coli of the gene encoding an
            extracellular deoxyribonuclease (DNase) from Aeromonas hydrophila
  JOURNAL   Gene (1992) In press
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     -35_signal      163..168
                     /gene="dns"
     -10_signal      187..192
                     /gene="dns"
     CDS             238..930
                     /product="deoxyribonuclease"
                     /gene="dns"
                     /note="putative"
                     /codon_start=1
                     /translation="MFRPLLSLCLALLVSAPAHADNIQTFRAAKQDLNKLYQSHPVTF
                     YCGCNIKFSGKKMAPDWESCGYLPGKQAERASRIEWEHVVPAWEFGHQLQCWQDGGRK
                     NCGKSDEFNRMEGDMHNLFPAIGEVNGDRANFRFSDWNGKPNQYGKCQMLVDFKERQV
                     QPPKGPVRGQIARAYLYMSQQYGLRLAAQQRKLYEAWDRQYPADRWECERNRRIGKLQ
                     GNTNPFIEKQCQ"
     sig_peptide     238..297
                     /gene="dns"
                     /codon_start=1
     mat_peptide     298..927
                     /product="deoxyribonuclease"
                     /gene="dns"
                     /codon_start=1
BASE COUNT      226 a    384 c    347 g    204 t
ORIGIN
        1 ggatctgggg gtgctggccc agcgcacctt cgtgtatgcc tgcgcctgcc agcagcatcc
       61 cctctcggtg cgccgccaca acaaggtgat gcgcggcgag gcccgctatc actgccgccg
      121 ctgtcgccag ccccttgggt ggcagcgcga cacgacggcg gattgagtca ccccgtgtcg
      181 ctcctgtgta agggtgcgcg cttacttcat tcaactgtct cattgccaag gatattcatg
      241 tttcgccctc tgctctccct ctgcctggcc ctgctggtca gcgccccggc ccatgccgac
      301 aacatccaga cctttcgcgc cgccaagcag gatctgaaca agctctacca gagccaccct
      361 gtcaccttct actgcggctg caacatcaag ttcagcggca agaagatggc ccccgactgg
      421 gagagctgcg gctacctgcc cggcaaacag gccgagcggg cctcccgcat cgaatgggag
      481 cacgtggtgc ccgcctggga gttcggccac cagctccagt gctggcagga cgggggtcgc
      541 aagaattgcg gcaagagcga cgagttcaac aggatggagg gagacatgca caacctcttc
      601 cctgccatcg gcgaggtgaa cggcgatcgg gcgaacttcc gcttcagcga ctggaacggc
      661 aaaccgaacc agtacggcaa gtgccagatg ctggtggact tcaaggagcg ccaggtgcag
      721 ccgcccaagg ggccggtgcg tggccagatc gcccgcgcct acctctacat gagccagcag
      781 tacggactgc gtctggcggc ccagcagcgc aagctttacg aagcctggga caggcagtat
      841 ccggctgatc gctgggagtg cgagcgcaac cgccgtatcg gcaagctgca gggcaatacc
      901 aatcccttca ttgaaaagca gtgccaataa ccccatttga ccagggaggg ggagcgtccc
      961 cctcctcctg aatccttcac tctctgtctc gcgagccttg ctcgccgccg tgcagtaggg
     1021 tattgaaggg tttccaagca agagactgac tatcagccat gcgcattcca cgtatctatg
     1081 aaccggccag cctgcaggtg ggccagaccc tcgccctgtc ggaagacggt gccaatcaca
     1141 tcggccgcgt gctgcgcatg c
//