From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: D.pseudoobscura Adh gene for alcohol dehydrogenase
Date: 28 Dec 92 21:27:05 GMT
Distribution: bionet
Checksum: 10759 8

LOCUS       DROADHGN36   3481 bp ds-DNA             INV       01-DEC-1992
DEFINITION  D.pseudoobscura Adh gene for alcohol dehydrogenase
ACCESSION   X62216
KEYWORDS    ADH gene; alcohol dehydrogenase.
SOURCE      Drosophila pseudoobscura DNA.
  ORGANISM  Drosophila pseudoobscura
            Eukaryota; Animalia; Metazoa; Arthropoda; Uniramia; Insecta;
            Pterygota; Neoptera; Holometabola; Diptera; Brachycera;
            Cyclorrhapha; Schizophora; Drosophiloidea; Drosophilidae.
REFERENCE   1  (bases 1 to 3481)
  AUTHORS   Schaeffer,S.W. and Miller,E.L.
  TITLE     Estimates of gene flow in Drosophila pseudoobscura determined from
            nucleotide sequence analysis of the alcohol dehydrogenase region
  JOURNAL   Genetics 132, 471-480 (1992)
  STANDARD  full automatic
COMMENT     *source: strain=GB96; *source: isolate=Gundlach Bundschu Winery,
            Sonoma Valley, *source: California; *source: chromosome=IV section
            88;
               From EMBL    entry DPADHGN36;  dated 16-OCT-1992.
FEATURES             Location/Qualifiers
     mRNA            join(40..119,915..1020,1084..1488,1551..1966)
                     /note="adult mRNA (Adh)"
     mRNA            join(882..1020,1084..1488,1551..1966)
                     /note="larval mRNA (Adh)"
     promoter        8..14
                     /note="adult promoter (Adh)"
     prim_transcript 40..1966
                     /note="adult primary transcript (Adh)"
     exon            40..119
                     /note="adult exon 1a"
     intron          120..914
                     /note="adult intron"
     promoter        846..853
                     /note="larval promoter (Adh)"
     prim_transcript 882..1966
                     /note="larval primary transcript (Adh)"
     exon            882..1020
                     /note="larval exon 1"
     exon            915..1020
                     /note="adult exon 1b"
     intron          1021..1083
                     /number=1
     exon            1084..1488
                     /number=2
     intron          1489..1550
                     /number=2
     exon            1551..1966
                     /number=3
     polyA_signal    1943..1948
     polyA_site      1966
     CDS             join(928..1020,1084..1488,1551..1817)
                     /EC_number="1.1.1.1"
                     /gene="Adh"
                     /product="alcoholdehydrogenase"
                     /codon_start=1
                     /translation="MSLTNKNVVFVAGLGGIGLDTSRELVKRNLKNLVILDRIDNPAA
                     IAELKAINPKVTITFYPYDVTVPVAETTKLLKTIFAQVKTIDVLINGAGILDDHQIER
                     TIAVNYTGLVNTTTAILDFWDKRKGGPGGIICNIGSVTGFNAIYQVPVYSGSKAAVVN
                     FTSSLAKLAPITGVTAYTVNPGITKTTLVHKFNSWLDVEPRVAEKLLEHPTQTSQQCA
                     ENFVKAIELNKNGAIWKLDLGTLEPITWTQHWDSGI"
BASE COUNT      980 a    793 c    794 g    914 t
ORIGIN      
        1 cgtagtgtat ttaaggcact gcacatgtcg aggaccacga ttattgtcta cagtgcagtc
       61 agtagttgtc aggtgcagca ggcggaccag catttcttta aatctactca atcgatcaag
      121 taagtaactg taatccaagt atagtcggcc catcccactc ggttctgccg taactcaaga
      181 aatttcttga tagcgcagtt ccagttgtaa aacgtcgttt aaaaaaacat ctcgttggag
      241 cttgggttga aaaacgttcg agttgggcgt agaaaatttc aaatataaaa tatcaaaaaa
      301 gagaaaatat cttcccaaag cagctgtcca ttttttgtta aagtgataga tcaaaaattc
      361 gtatgacgta tttcccaagt gctctttatg caaataattt cctcaattct aattctacaa
      421 gaagcacttt tggcacttgt ccagaaaaaa caatttgttt acacttttcg gttgataaca
      481 gaggcggacc gacggctaag taacataaca gtcgcctgtt aagtaatgcc ctcactctct
      541 gttagatggc cgcgcgtgtc gactccgata gaccagcaga cacgcagtag ttttcctcaa
      601 gaacctgaac tctaaacata gacataattt acgatacctt gagactcaaa taaaaataca
      661 agtatgagtg gtattctatt cctgattaga ctcgcacaca cacacgtacg agtattaaca
      721 taaatttgcc ggcaaataac caaaacaaaa cgaacgcaaa cgatccgaac acgtaatgcg
      781 agagataaga aacagaaaag ctcgacgtga aagaagcttc tggcggaagc tcttgccgaa
      841 ctccctataa atacgagaca gaaaaccaga ataaatataa taacaaactg ttgaattgtc
      901 ccatcgattc ccagatcaaa agacagaatg tctctcacca acaagaacgt cgttttcgtg
      961 gccggtctgg gcggcattgg cctggacacc agccgggagt tggtcaagcg taatctgaag
     1021 gtaagagaga acgaattcca tggaatctat ggaatcctaa atttaaaaat tcatttattt
     1081 tagaacctgg tcatcctgga tcgcattgac aatccggctg ccattgccga actgaaggca
     1141 atcaatccca aggtgaccat caccttctat ccctacgatg tgactgtgcc cgtcgctgag
     1201 accaccaagc tcctgaagac catttttgcc caggtgaaga ccatcgatgt cctgatcaac
     1261 ggtgctggca tcctggacga tcatcagatc gagcgcacca ttgccgttaa ctacacgggc
     1321 ctggtcaaca ccaccacagc cattctggac ttctgggaca agcgcaaggg cggcccaggc
     1381 ggcatcattt gcaacattgg ctccgtcacc ggtttcaatg ccatctacca ggtgcccgtt
     1441 tactccggct ccaaggcggc ggtggttaac tttacctcct ccctggcggt aagaaatcct
     1501 acaactatcc taactatccc aaaactaaat atttctttaa aatcccatag aaactggctc
     1561 ccattactgg tgtcactgct tacactgtca atcctggcat cactaagacc actctggtcc
     1621 acaaattcaa ctcgtggctg gatgtggagc cccgtgtggc agagaagctg ctcgagcatc
     1681 ccacccagac ctctcagcag tgcgccgaga actttgtgaa ggccatcgag ctgaacaaga
     1741 acggtgccat ctggaagttg gatttgggca ccttggagcc catcacatgg acccagcact
     1801 gggactcggg catctaaacc atcccagaga ctctatggga catggcgttt tagcttttag
     1861 tttctttttt tccactcaat tgttacgtat atacatacac atatggcaat aaggctgatt
     1921 tgaaccccgt tttgaatatg ataatacaaa attatatttg agaaatttca acaaaatcga
     1981 taagagctat agctctcttt taattaaaaa aaactagata aaaagagcaa tggtcagtgg
     2041 tggtggtcag tggtcgttgt ggcctctatc gatttccaca caaaaacttt actgtttagt
     2101 aatagaaaag aacttgagaa aggcagccaa aagaatgtac gatctgacgg gtaagcatgt
     2161 ctgctatgtg gctgactgcg gtggcattgc actggagacc agcaaggttc tcatgaccaa
     2221 gaatatagcg gtgagtggta gagaggtggt gtagagagtg gagagtggtt ctggttctta
     2281 aacaagtctc tagtctctag tctctagtct cttggctgct gctggtagct gtcgaaacta
     2341 attaagttaa tgactttttc atgttttgtt tttgtccgct gacttttgct gttcaaagct
     2401 acaattaagt ttatgttttg agtttgatct acttttgctt aagggaagta ctttcgatta
     2461 cggattgctg gcatataaaa atagatacca aggaatgtgc atcatatcat attgattcta
     2521 tcttgattct ttgcccagaa actggccatc cttcagagcg tggaaaaccc cccggccatc
     2581 gctcagttac aatcgattaa gcacagcaca cagatcttct tctggacctt cgatgtgacc
     2641 atggcccgcg aggagatgaa gaagtacttc gatgaggtca tggtccagat ggactacatc
     2701 gatgtgctga tcaatggggc caccctgtgc gacgagcgga acattgatgc caccatcaat
     2761 acgaatctga ccggcatgat gaacaccgtg gccactgtgc tgccctatat ggaccgaaag
     2821 atgggcggtt cgggtggact gattgtgaat gtcacctccg tcataggact ggatccatcg
     2881 ccagtctttt gtgcgtacag tgcctcaaag tttggtgtga ttgggttcac ccgaagtcta
     2941 gcggtgagtg ggcgagatcc taacttgaaa atgctctcta atgtcttcca ttctttcgca
     3001 ggatcccctg tattacaccc aaaatggtgt ggctgtcatg gccgtctgct gtggtcccac
     3061 caaagtgttt gtcgatcggg aactgactgc cttcctggaa tacggccaaa cctttgccga
     3121 tcgcctgcgt cgtgccccct gccaatcgac ggccgtctgc ggccagaata tagtaaatgc
     3181 catcgagaga tcggagaacg gacagatctg gattgccgac aagggcggcc tggagtcggt
     3241 ggccctccac tggtattggc atatggccga tcagtttgtg aactatatgc agagcactga
     3301 cgatgaggat caggagtttt ttttgggtca gcgatgaggc atgccttgat ccacggaata
     3361 aaacatcaaa tgtgtcacaa aaagtaggca acttgtttgt gggtctggaa atagatttga
     3421 aatattttga ggtcatatga ataatgacat aaatgagggt gcctgaaagt atgccaagtt
     3481 t
//