From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: phbACv=acetyl-CoA acyltransferase...orf 7 3' to phbB [Chromatium
Date: 12 Jan 93 05:48:14 GMT
Distribution: bionet
Checksum: 18151 8

LOCUS       M246906S1    3200 bp    DNA             BCT       11-JAN-1993
DEFINITION  phbACv=acetyl-CoA acyltransferase...orf 7 3' to phbB [Chromatium
            vinosum, strain D, Genomic, 7 genes, 3200 nt, segment 1 of 2] 
ACCESSION   S46163
KEYWORDS    .
SEGMENT     1 of 2
SOURCE      Chromatium vinosum strain D
  ORGANISM  Chromatium vinosum
            Unclassified.
REFERENCE   1  (bases 1 to 3200)
  AUTHORS   Liebergesell,M. and Steinbuchel,A.
  TITLE     Cloning and nucleotide sequences of genes relevant for biosynthesis
            of poly(3-hydroxybutyric acid) in Chromatium vinosum strain D.
  JOURNAL   Eur. J. Biochem. 209, 135-150 (1992)
  STANDARD  full automatic
COMMENT     This entry [NCBI gibbsq 115522] was created by the journal scanning
            component of NCBI/GenBank at the National Library of Medicine.
            This sequence comes from Fig. 2.
FEATURES             Location/Qualifiers
     CDS             complement(832..1899)
                     /note="mismatches(113[A->G],251[P->D],331[I->H],
                     332[H->I]).  PHA synthase;  For the protein sequence (NCBI
                     gibbsq 115524):  Method: conceptual translation supplied
                     by author.  This sequence comes from Fig. 2.
                     Author-given protein sequence is in conflict with the
                     conceptual translation"
                     /gene="phbCCv"
                     /product="poly(3-hydroxyalkanoate) synthase"
                     /codon_start=1
                     /translation="MFPIDIRPDKLTQEMLDYSRKLGQGMENLLNAEAIDTGVSPKQA
                     VYSEDKLVLYRYDRPEGAPEAQPVPLLIVYALVNRPYMTDIQEDRSTIKGLLATGQDV
                     YLIDWGYPDQADRALTLDDYINGYIDRCVDYLREAHGVDKVNLLGICQGGAFSLMYSA
                     LHPDKVRNLVTMVTPVDFKTPDNLLSAWVQNVDIDLAVDTMGNIPGELLNWTFLSLKP
                     FSLTGQKYVNMVDLLDDPDKVKNFLRMEKWIFPSPDQAGETFRQFIKDFYQNNGFLNG
                     GVVLGGQEVDLKDITCPVLNIFALQDHLVPPDASRALKGLTSSPDYTELAFPGGIHGI
                     YVSGKAQKEVTPAIGKWLNER"
     CDS             complement(1957..3030)
                     /note="mismatches(40[T->N],292[P->R]).  Description: orf 2
                     5' to phbA;  For the protein sequence (NCBI gibbsq
                     115525):  Method: conceptual translation supplied by
                     author.  This sequence comes from Fig. 2.
                     Author-given protein sequence is in conflict with the
                     conceptual translation"
                     /codon_start=1
                     /translation="MSNTNFFNDDWLELQRKYWDNWTDMSRKAMGLDSASSSATTPWE
                     AAIDQWWKAMAPAAPDLSRSFMEKMMEQGKNFFRLADTFAKRADEGNAGNGLELWTKT
                     LEDMQKRFSGSLDDGGNTMQRLMSFWELPLDNWQRMMSSMSPMPGDMLRNMPHEQFKD
                     SLDRALSAPGLGYTREEQSQYQELMRSAMEYQAALQEYTNVYTKLGMKSVEHMGSYIQ
                     GVIDSGKTIDSARALYDNWVACCEGAYADEVATPEYARIHGRLVNAQMALKKRMSILV
                     DENLGALNMPTRSELPTLQDRLQETRRENKALRHSLRDLERRVAALAGEEPATKPATA
                     LRSPAPAAKAPARRRTTKTNPAD"
BASE COUNT      527 a    947 c   1048 g    678 t
ORIGIN      
        1 cgaattcatc cagggccact atctccaact gccgagttcg gacttgaact tcgatttcca
       61 gaccctgtca catgaaaccc gatagtctgc tcatgagtac ggcgcttcac acgggacggc
      121 tgatcctgac tccatccgat ccgcatctcg cgccggatgt cgatttgttg atcgaaggac
      181 tgagccggcc tggattcctg agtgctccat tgatcctgga gaatccacag ccgggggcgg
      241 gcgaccgagc ctttgcgact ggcgccggct tcctgagtct actgaccttt gccggctgcg
      301 ccgtccggat tcgcgatgac gtcagagcgg ggcgtttctc gcatattcgc atcccgccag
      361 tcagtcagca tccaaggttg atggttggcc gtaacacgcg cccaccccga tgtgtcgggt
      421 gtcgcgcgcc cctgtcgggc tggcgcgcgc tggtggaaca ctgggccacg catccgcatg
      481 ccggcgtgca atgtccggcc tgcggcgaga cacgtccgcc ctggctctgg gactggaaac
      541 agcaaggagg attcggacgc gccttcgtgc agatcgaaga ggtctttccg ggtgaggcag
      601 tgccgacgcc gatgttcttc gagcagctca ttcgggtcag tgggatcggc tggcgacatt
      661 tctacatcca ggattgaatc aagcctggta taggggcgcc ttaacgtgaa ggccagctcc
      721 aagtccgctc gatgagcgac gacgcccggt gagtcccgag cgtcatcgtg gatgccgttc
      781 gacggctgac gacagcgagc cgccggaatt gctccggcgg ctcgggaagc gttatcgctc
      841 gttgagccac ttaccgatgg ccggcgtgac ttctttctgc gccttgccgc tgacatagat
      901 gccgatgtga ccgccgggga aggccagctc ggtgtagtcc gggctgctgg tcagtccctt
      961 gagcgcccta gaggcatccg gcggcaccag gtgatcctgg agcgcgaaga tgttgagcac
     1021 cgggcaggtg atgtccttca ggtccacttc ctgtccgccc agcaccacgc cgccattgag
     1081 gaagccgttg ttctggtaga agtccttgat gaactgacgg aaggtctcgc cggcctgatc
     1141 cgggctgtcg aagatccatt tctccatgcg caggaagttc ttgaccttgt ccgggtcgtc
     1201 gagcagatca accatgttga cgtacttctg acccgtcagg ctgaaaggct tgagcgacag
     1261 gaaggtccag ttcagcagct caccggggat gttgcccatg gtgtcgaccg ccagatcgat
     1321 gtcgacgttc tgcacccagg ccgagagcag gttgtccggg gtcttgaagt cgaccggcgt
     1381 gaccatggtc acgaggttgc gcaccttgtc cgggtgcagc gcgctgtaca tcaggctgaa
     1441 ggcgccgccc tgacagatac cgagcagatt gaccttgtcg acgccgtggg cttcgcgcag
     1501 atagtccacg cagcggtcga tgtagccgtt gatgtagtcg tcgagcgtga gcgcgcggtc
     1561 gccctgatcc ggatagcccc agtcgatcag gtagacgtcc tggccggtgg ccagcagtcc
     1621 cttgatggtg gagcggtctt cctggatgtc ggtcatgtag ggccggttga ccagggcgta
     1681 gacgatcagg agcggcacgg gctgggcttc gggcgcgccc tcgggtcggt catagcgata
     1741 gaggaccagc ttgtcctcgc tgtagacggc ctgcttgggg ctgacgccgg tgtcgatggc
     1801 ctcggcgttg agcagattct ccatgccctg acccagcttg cggctgtagt cgagcatctc
     1861 ctgggtgagt ttgtccggcc ggatgtcgat ggggaacatg atcgctgtcc tctgattctg
     1921 tctggtgagc ctgctgttcg atcagccgct tgacgctcaa tcggccggat tggtcttggt
     1981 ggtgcgccgg cgtgccggag ccttggcggc cggggcgggc gacctgagcg cagtggcagg
     2041 cttggtggcc ggctcctcac cggccagggc tgcgacgcga cgttccagat cgcgcaggct
     2101 gtgacgcagg gccttgttct cgcgacgggt ctcctggagc cggtcctgga gggtgcgcag
     2161 ctcgctgcgg gtcggcatgt tcaatgcgcc caggttctcg tcgaccagga tcgacatgcg
     2221 cttcttgagc gccatctgag cgttgaccag ccggccgtgg atgcgggcgt attccggtgt
     2281 ggcgacctcg tcggcatagg cgccttcgca gcaggcgacc cagttgtcgt agagcgcgcg
     2341 cgccgagtcg atggttttgc cgctgtcgat gacaccctgg atgtaggagc ccatgtgctc
     2401 gaccgacttc atgccgagct tggtgtagac gttggtgtat tcctggagcg cggcctgata
     2461 ctccatggcg ctgcgcatca gctcctgata ctggctctgc tcctcgcggg tgtagccgag
     2521 accgggcgcc gagagggctc gatcaagact gtccttgaac tgctcgtggg gcatgttgcg
     2581 cagcatgtcg cccggcatcg gcgacatcga ggacatcatg cgctgccagt tgtcgagcgg
     2641 cagctcccag aacgacatca ggcgctgcat ggtgttgccg ccgtcgtcga gcgagccgct
     2701 gaagcgcttc tgcatgtctt cgagcgtctt ggtccagagc tccaagccgt tgccggcgtt
     2761 gccctcgtcg gcgcgtttgg cgaaggtgtc ggcgagacgg aagaagttct tgccctgctc
     2821 catcatcttt tccatgaacg agcgtgacag atccggggcg gcgggcgcca tggctttcca
     2881 ccactgatcg atggccgcct cccagggcgt gttggccgaa ctggaggcgc tgtccagacc
     2941 catggccttg cggctcatgt cggtccagtt gtcccagtac ttgcgttgca gttcgagcca
     3001 atcgtcattg aagaaattag tgttgctcat ggacgtaccc tcctcgtggc gcgtggtgtt
     3061 gggcgaacgg tatcatgtgg atttgtgcac tgcaacaaag atgcgaagaa tccacaggcc
     3121 gcgaccccat atcggggacg ctcgtccatt tccaatcaga atccagagga catccacgcc
     3181 atgagcgaga acatcgtcat
//