From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: R.meliloti ORF1, complete cds; ORF2, complete cds; and
Date: 28 Dec 92 21:45:26 GMT
Distribution: bionet
Checksum: 58066 5

LOCUS       RHMDGK       1258 bp ds-DNA             BCT       02-DEC-1992
DEFINITION  R.meliloti ORF1, complete cds; ORF2, complete cds; and
            diacylglycerol kinase (dgkA) gene, complete cds.
ACCESSION   M94085
KEYWORDS    diacylglycerol kinase.
SOURCE      Rhizobium meliloti (strain 1021) DNA.
  ORGANISM  Rhizobium meliloti
            Prokaryotae; Gracilicutes; Scotobacteria; Rhizobiaceae.
REFERENCE   1  (bases 1 to 1258)
  AUTHORS   Miller,K.J., McKinstry,M.W., Hunt,W.P. and Nixon,B.T.
  TITLE     Identification of the diglyceride kinase structural gene of
            Rhizobium meliloti 1021
  JOURNAL   Mol. Plant Microb. Interact. 5, 363-371 (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     CDS             90..716
                     /citation=[1]
                     /note="putative;  not homologous to known sequences as of
                     2/92; ORF1"
                     /codon_start=1
                     /translation="MGRPRYRFGRRGAHAQDRCCRKGRCAAPRPLVRSARSHAAAWRP
                     RDRGDGGRNSRRADAEGARHHRRLRRDRGRGDPQGRQPGGARPLPDRPCFKRARSHAG
                     DRETRQDTAPGSRHAAWRRHGCGSCSRHRQGGGCLPQRHGDLCAGRRKQQGVGHAPAL
                     AVDWPSCVLCSCRAAIDAGRLLAYPGSAGSSVRAASQCGIGAAFDSPQ"
     CDS             130..552
                     /citation=[1]
                     /note="putative;  not homologous to known sequence as of
                     2/92; ORF2"
                     /codon_start=1
                     /translation="MRKIAAVEKAVALHRDHLSDPLEVMRRLGGREIAAMAGAILAAR
                     MQKVPVIIDGYVATAAAAILKAANPAALDHCLIGHVSSEPGHMRAIEKLGKTPLLALG
                     MRLGEGTGAALAAGIVKAAAACHSGMATFAQAGVSNKE"
     CDS             757..1170
                     /function="ATP-dependent phosphorylation of sn-1,
                     2-diacylglycerol"
                     /EC_number="2.7.1.107"
                     /standard_name="DGK"
                     /gene="dgkA"
                     /citation=[1]
                     /note="produces phosphatidic acid to recycle
                     diacylglycerol. Phenotype of null mutant in E. coli is
                     sensitivity toarbutin at low osmolarity; putative"
                     /product="diacylglycerol kinase"
                     /codon_start=1
                     /translation="MDAKNTTSRNGTAAPVHKHRGIRHLFAAASYSFGGAKRLIGEAA
                     FRHELIAFAVAMVAFMIVGANLFQYVAMAILFLLMMAFEAINTAIEEIVDRVSPEISE
                     MGRNAKDLGSFACLCLILANAAYALYVIFLARFLG"
BASE COUNT      218 a    401 c    408 g    231 t
ORIGIN      
        1 gatctgctgt gcatcggcga gatgggcatc ggcaacacca cgatcgcggc cgcaatcaat
       61 ctcggcctct atggcggcac ggcagaggaa tgggtcggcc ccggtaccgg ttcggaaggc
      121 gaggtgctca tgcgcaagat cgctgctgtc gaaaaggccg ttgcgctgca ccgcgaccac
      181 ttgtccgatc cgctcgaagt catgcggcgg cttggcggcc gcgagatcgc ggcgatggcg
      241 ggcgcaattc tcgccgcgcg gatgcagaag gtgcccgtca tcatcgacgg ctacgtcgcg
      301 accgcggccg cggcgatcct caaggccgcc aacccggcgg cgctcgacca ttgcctgatc
      361 ggccatgttt caagcgagcc cggtcacatg cgggcgatcg agaaactcgg caagacaccg
      421 ctcctggctc tcggcatgcg gcttggcgaa ggcacgggtg cggctcttgc agcaggcatc
      481 gtcaaggcgg cggctgcctg ccacagcggc atggcgacct ttgcgcaggc cggcgtaagc
      541 aacaaggagt agggcacgct ccggcgcttg ctgtcgattg gccgtcatgc gtgctatgct
      601 cgtgccgtgc agctattgat gcggggcggc ttctcgcata tccgggaagc gccgggtcat
      661 cggttcgggc ggcctcgcaa tgcggcattg gtgccgcctt tgattcgcct caataggaac
      721 gtcgaagctg tgccatctcc caacccgcga gacgaaatgg acgccaagaa cactacctcc
      781 cgcaacggca ccgccgcgcc ggtccacaag catcgtggta tccggcatct tttcgccgcg
      841 gccagctatt ccttcggcgg tgcgaaacgc ctgatcggcg aggcggcatt caggcacgaa
      901 ctcatcgcct tcgcggtcgc gatggtcgcg ttcatgatcg tcggcgcgaa cctgttccag
      961 tacgtcgcga tggcaatcct gtttctgctg atgatggcct tcgaggcgat caacaccgcg
     1021 atcgaggaga tcgtcgaccg cgtctcgccg gagatctccg aaatgggcag gaacgcaaag
     1081 gatctcggct ccttcgcgtg cctttgcctc attctcgcca atgcggccta tgcgctgtac
     1141 gtgatatttc tcgctcgctt cctgggctga ccagcaacgc ggcgccgcat gcagatgatc
     1201 aggcaaagct ccttcgccgg cgggtgaggg aagggacgtc ctcgaccggg ggcaagct
//