From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: D.pseudoobscura Adh gene for alcohol dehydrogenase
Date: 28 Dec 92 21:28:00 GMT
Distribution: bionet
Checksum: 54948 8

LOCUS       DROADHGN73   3498 bp ds-DNA             INV       01-DEC-1992
DEFINITION  D.pseudoobscura Adh gene for alcohol dehydrogenase
ACCESSION   X64482
KEYWORDS    ADH gene; alcohol dehydrogenase.
SOURCE      Drosophila pseudoobscura DNA.
  ORGANISM  Drosophila pseudoobscura
            Eukaryota; Animalia; Metazoa; Arthropoda; Uniramia; Insecta;
            Pterygota; Neoptera; Holometabola; Diptera; Brachycera;
            Cyclorrhapha; Schizophora; Drosophiloidea; Drosophilidae.
REFERENCE   1  (bases 1 to 3498)
  AUTHORS   Schaeffer,S.W. and Miller,E.L.
  TITLE     Estimates of gene flow in Drosophila pseudoobscura determined from
            nucleotide sequence analysis of the alcohol dehydrogenase region
  JOURNAL   Genetics 132, 471-480 (1992)
  STANDARD  full automatic
COMMENT     *source: strain=PS274; *source: isolate=Kiabab National Forest,
            Arizona; *source: chromosome=IV section 88;
               From EMBL    entry DPADHGN73;  dated 16-OCT-1992.
FEATURES             Location/Qualifiers
     mRNA            join(40..119,915..1020,1101..1505,1566..1981)
                     /note="adult mRNA (Adh)"
     mRNA            join(882..1020,1101..1505,1566..1981)
                     /note="larval mRNA (Adh)"
     promoter        8..14
                     /note="adult promoter (Adh)"
     prim_transcript 40..1981
                     /note="adult primary transcript (Adh)"
     exon            40..119
                     /note="adult exon 1a"
     intron          120..914
                     /note="adult intron"
     prim_transcript 882..1981
                     /note="larval primary transcript (Adh)"
     exon            882..1020
                     /note="larval exon 1"
     exon            915..1020
                     /note="adult exon 1b"
     intron          1021..1100
                     /number=1
     exon            1101..1505
                     /number=2
     intron          1506..1565
                     /number=2
     exon            1566..1981
                     /number=3
     polyA_signal    1958..1963
     polyA_site      1981
     CDS             join(928..1020,1101..1505,1566..1832)
                     /EC_number="1.1.1.1"
                     /gene="Adh"
                     /product="alcoholdehydrogenase"
                     /codon_start=1
                     /translation="MSLTNKNVVFVAGLGGIGLDTSRELVKRNLKNLVILDRIDNPAA
                     IAELKAINPKVTITFYPYDVTVPVAETTKLLKTIFAQVKTIDVLINGAGILDDHQIER
                     TIAVNYTGLVNTTTAILDFWDKRKGGPGGIICNIGSVTGFNAIYQVPVYSGSKAAVVN
                     FTSSLAKLAPITGVTAYTVNPGITKTTLVHKFNSWLDVEPRVAEKLLEHPTQTSQQCA
                     ENFVKAIELNKNGAIWKLDLGTLEPITWTQHWDSGI"
BASE COUNT      981 a    796 c    802 g    919 t
ORIGIN      
        1 cgtagtgtat ttaaggcact gcacatgtcg aggaccacga ttattgtcta cagtgcagtc
       61 agtagttgtc aggtgcagca ggcggaccag catttcttta aatctactca atcgatcaag
      121 taagtaactg taatccgagt acagtcgacc catcccactc ggttctgcag taactcaaga
      181 aatttcttga tagcgcagtt ccagttgtaa aacgtcgctt aaaaaaacat ctcgttggag
      241 cttgggttga aaaacgttcg agttgggcgt agaaaagctc aataataaaa tatcaaaaaa
      301 gagaaaatat cttcccaaag cagctgtcca ttttttgtta aagtgataga tcaaaaattc
      361 gtatgacgta tttcccaagt gctcttcatg caaataatgt cctcaattct aattctacaa
      421 gaagcacttt tggctcttgt ccagaaaaaa caatttgttt acacttttcg gttgataaca
      481 gaggcggacc gacggctaag taacataaca gtcgcctgtt aagtaatgcc ctcactctct
      541 gttagatggc cgcgcgtgtc gactccgata gaccagcaga cacgcagtag ttttcctcaa
      601 gaacctgaac tctaaacata gacataattt acgatacctt gagactcaaa taaaaataca
      661 tgtatgagtg gtattctatt cctgattaga ctcgcacaca cacacgtacg agtattaaca
      721 taaatttgcc ggcaaataac caaaacaaaa cgaacgcaaa cgatccgaac acgtaatgcg
      781 agagataaga aacagaaaag ctcgacgtga aagaagcttc tggcggaagc tcttgccgaa
      841 ctccctataa atacgagaca gaaaaccaga ataaatataa taacaaactg ttgaattgtc
      901 ccatcgattc ccagatcaaa agacagaatg tctctcacca acaagaacgt cgttttcgtg
      961 gccggtctgg gcggcattgg cctggacacc agccgggagt tggtcaagcg taatctgaag
     1021 gtaagagaga tggagagaga gagcaatccc ttttccatgg aatctatgga atcctaaatt
     1081 taaaaattca tttattttag aacctggtca tcctggatcg cattgacaat ccggctgcca
     1141 ttgccgaact gaaggcaatc aatcccaagg tgaccatcac cttctatccc tacgatgtga
     1201 ctgtgcccgt cgctgagacc accaagctcc tgaagaccat ctttgcccag gtgaagacaa
     1261 tcgatgtcct gatcaacggt gctggcatcc tggacgatca tcagatcgag cgcaccattg
     1321 ccgttaacta cacgggcctg gtcaacacca ccacagccat tctggacttc tgggacaagc
     1381 gcaagggcgg cccaggcggc atcatttgca acattggctc cgtcaccggt ttcaatgcca
     1441 tctaccaggt gcccgtttac tccggctcca aggcggcggt ggttaacttt acctcctccc
     1501 tggcggtaag taatcctaca actatcctac tccccaaaac taaatatttc tttgaaatct
     1561 catagaaact ggctcccatt actggtgtca ctgcttacac tgtcaatcct ggcatcacta
     1621 agaccactct ggtccacaaa ttcaactcgt ggctggatgt ggagccccgt gtggcagaga
     1681 agctgctcga gcatcccacc cagacctctc agcagtgcgc cgagaacttt gtgaaggcca
     1741 ttgagctgaa caagaacggt gccatctgga agttggatct gggcaccttg gagcccatca
     1801 catggaccca gcactgggat tcgggcatct aaaccatccc agagactcta tgggacatgg
     1861 cgttttagct tttagtttct ttttttccac tcaattgtta cgtatataca tacacatatg
     1921 gcaataaggc tgatttgaac cccgttttga atatgataat acaaaattat atttgagaaa
     1981 tttcaacaaa atcgataaga gctctagctc tcttttaatt aaaaaaaact agataaaaag
     2041 agcaatggtc agtggtggtc agtggtcgtt gtggcctcta tcgatttcca cacaaaaact
     2101 ttactgttta gtaatagaaa agaacttgag aaaggcagcc aaaagaatgt acgatctgac
     2161 gggtaagcat gtctgctatg tggctgactg cggtggcatt gcactggaga ccagcaaggt
     2221 tctcatgacc aagaatatag cggtgagtgg tagagtggtg tagagagtgg agagtgttgc
     2281 gaagtggttc tggttcttaa acaagtctct agtctctagt ctctagtctc ttggctgctg
     2341 ctggtagctg tcgaaactaa ttaagttaat gactttttca tgttttgttt ttgtccgctg
     2401 acttttgctg ttcaaagcta caattaagtt tatgttttga gtttgatcta cttttgctta
     2461 agggaagtac tttcgattac ggattgctgg catataaaaa tagataccaa ggaatgtgca
     2521 tcatatcata ttgattctat cttgattcta tctagaaact ggccatcctt cagagcgtgg
     2581 aaaacccccc ggccatcgct cagttacaat cgattaagca cagcacacag atcttcttct
     2641 ggaccttcga tgtgaccatg gcccgcgagg agatgaagaa gtacttcgat gaggtcatgg
     2701 tccagatgga ctacatcgat gtactgatca atggggccac cctgtgcgat gagcggaaca
     2761 ttgatgccac catcaatacg aatctgaccg gcatgatgaa caccgtggcc actgtgctgc
     2821 cctatatgga ccgtaagatg ggcggttcgg gtggactgat tgtgaatgtc acctccgtca
     2881 taggactgga tccatcgcca gtcttttgtg cgtacagtgc ctcaaagttt ggtgtgattg
     2941 ggttcacccg aagtctagcg gtgagtgggc gagatcttaa cttgaaaatc ctctctaatg
     3001 tcttccattc ttcggcagga tcccctgtat tacacccaaa atggtgtggc tgtcatggcc
     3061 gtctgctgtg gtcccaccaa agtgtttgtc gatcgggaac tgactgcctt cctggcatac
     3121 ggccaaacct ttgccgatcg cctgcgtcgt gccccctgcc aatcgacggc cgtctgcggc
     3181 aagaatatag taaatgccat tgagagatcg gagaacggac agatctggat tgccgacaag
     3241 ggcggcctgg agtcggtggc cctccactgg tattggcata tggccgatca gtttgtgaac
     3301 tatatgcaga gcactgacga tgaggatcag gagttttttt tggggcagcg atgaggcatg
     3361 ccttgatcca cggaataaaa catcaaatgt gtcacaaaaa gtaggcaact tgtttgtggg
     3421 tctggaaata gatttgaaat attttgaggt catatgaata atgacataaa tgagggtgcc
     3481 tgaaagtatg ccaagttt
//