From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: B.subtilis folic acid biosynthetic operon encoding
Date: 22 Dec 92 22:41:58 GMT
Distribution: bionet
Checksum: 21232 11

LOCUS       BACRFOLA     4880 bp ds-DNA             BCT       23-NOV-1992
DEFINITION  B.subtilis folic acid biosynthetic operon encoding
            para-aminobenzoic acid synthase, component I (pab), glutamine
            amidotransferase (trpG), pot. p-aminobenzoate synthesis enzyme
            (pabC), and pot. dihydropteroate synthase (sul) genes, complete
            cds.
ACCESSION   M34053
KEYWORDS    H2-pteridine-CH2OH pyrophosphokinase; dihydropteroate synthase;
            glutamine amido transferase; para-aminobenzoic acid synthase.
SOURCE      B.subtilis (strain VB157) vegetative cell DNA, clones pJS[1,2].
  ORGANISM  Bacillus subtilis
            Prokaryotae; Firmicutes; Endospore-forming rods and cocci;
            Bacillaceae.
REFERENCE   1  (bases 1 to 4880)
  AUTHORS   Slock,J., Stahl,D.P., Han,C.-Y., Six,E.W. and Crawford,I.P.
  TITLE     An apparent Bacillus subtilis folic acid biosynthetic operon
            containing pab, an amphibolic trpG gene, a third gene required for
            synthesis of para-aminobenzoic acid, and the dihydropteroate
            synthase gene
  JOURNAL   J. Bacteriol. 172, 7211-7226 (1990)
  STANDARD  full automatic
COMMENT     Draft entry and computer-readable sequence for [Unpublished (1990)]
            kindly submitted by D.P.Stahly, 07-MAY-1990.

              Author address: D.P.Stahly
              University of Iowa
              Dept. of Microbiology
              Iowa City, IA 52242
              e-mail: cmdsix@vaxa.weeg.uiowa.edu.
FEATURES             Location/Qualifiers
     RBS             300..303
                     /note="ribosome binding site"
     misc_binding    1720..1728
                     /bound_moiety="methyltryptophan resistance protein"
     RBS             1724..1730
                     /note="ribosome binding site"
     RBS             2306..2313
                     /note="ribosome binding site"
     RBS             3175..3179
                     /note="ribosome binding site"
     RBS             4023..4027
                     /note="ribosome binding site"
     RBS             4381..4385
                     /note="ribosome binding site"
     CDS             313..1725
                     /note="(pab)"
                     /note="para-aminobenzoic acid synthase, component I"
                     /codon_start=1
                     /translation="MAQRRPAGKKIPFQKDSFLQQFEKLAQSRKHHVLLESARGGRYS
                     IAGLDPIATVKGKDGITTIKHGDEMLFKEGDPLRAFHSWFKTLETETNHEFPDFQGGA
                     IGFLSYDYARYIENFKMLSLDDLETPDIYFLVFDDIAVYDHQEESLWLITHVNGSDQE
                     TADVKLSELEQMWLTELPAVTSREMKPETAGSFAAPFTEDGFSQAVEKIKQYIASGDV
                     FQVNLSIRQSQSLSVHPYQIYKTLREVNPSPYMAYLETPDFQIICGSPELLVSKKGKL
                     LETRPIAGTRSRGKTNEEDEALANELIHNEKERAEHVMLVDLERNDLGRVSRYGSVRV
                     NEFMAIEKYSHVMHIVSNVQGELQDGYDAVDIIHAVFPGGTITGAPKVRTMEIIEELE
                     PTRRGLYTGSIGWFGYNHDLQFNIVIRTIYATGGQAFMQSGAGVVIDSVPKHEYKESF
                     KKAFAMQRALELSEEETKIR"
     CDS             1739..2323
                     /note="(trpG)"
                     /note="glutamine amidotransferase"
                     /codon_start=1
                     /translation="MILMIDNYDSFTYNLVQYLGELGEELVVKRNDSITIDEIEELSP
                     DFLMISPGPCSPDEAGISLEAIKHFAGKIPIFGVCLGHQSIAQVFGGDVVRAERLMHG
                     KTSDIEHDGKTIFEGLKNPLVATRYHSLIVKPETLPSCFTVTAQTKEGEIMAIRHNDL
                     PIEGVQFHPESIMTSFGKEMLRNFIETYRKEVIA"
     CDS             2323..3204
                     /note="pot. p-aminobenzoate synthesis enzyme (pabC)"
                     /note="putative"
                     /codon_start=1
                     /translation="MIYVNGRYMEEKDAVLSPFDHGFLYGIGVFETFRLYEGCPFLLD
                     WHIERLERALKDLQIEYTVSKHEILEMLDKLLKLNDIKDGNARVRLNISAGISDKGFV
                     AQTYDKPTVLCFVNQLKPESLPLQKEGKVLSIRRNTPEGSFRLKSHHYLNNMYAKREI
                     GNDPRVEGIFLTEDGAVAEGIISNVFWRKGRCIYTPSLDTGILDGVTRRFIIENAKDI
                     GLELKTGRYELEALLTADEAWMTNSVLEIIPFTKIEEVNYGSQSGEATSALQLLYKKE
                     IKNMIHEKGGRAWRSTQ"
     CDS             3186..4043
                     /note="pot. dihydropteroate synthase (sul) (EC 2.5.1.15)"
                     /note="putative"
                     /codon_start=1
                     /translation="MAQHTIDQTQVIHTKPSALSYKEKTLVMGILNVTPDSFSDGGKY
                     DSLDKALLHAKEMIDDGAHIIDIGGESTRPGAECVSEDEEMSRVIPVIERITKELGVP
                     ISVDTYKASVADEAVKAGASIINDIWGAKHDPKMASVAAEHNVPIVLMHNRPERNYND
                     LLPDMLSDLMESVKIAVEAGVDEKNIILDPGIGFPKTYHDNLAVMNKLEIFSGLGYPV
                     LLATSRKRFIGRVLDLPPEERAEGTGATVCLGIQKGCDIVRVHDVKQIARMAKMMDAM
                     LNKGGVHHG"
     CDS             4036..4398
                     /note="ORF1"
                     /codon_start=1
                     /translation="MDKVYVEGMEFYGYHGVFTEENKLGQRFKVDLTAELDLSKAGQT
                     DDLEQTINYAELYHVCKDIVEGEPVKLVETLAERIAGTVLGKFQPVQQCTVKVIKPDP
                     PIPGHYKSVAIEITRKKS"
BASE COUNT     1512 a    939 c   1205 g   1224 t
ORIGIN      
        1 gaattctcgg aggcatctca tcaggtgcgg caatttatgc tgctcttcaa gtagcaaaaa
       61 aactcggaaa aggcaaaaaa gtattggcca tcattccaag taacggcgaa cgttatctaa
      121 gcacgccgct gtaccaattc gattaaaaaa agccaaaact cccggttcgc cgggagtttt
      181 tttatatttc gtgcatcaaa tatgccatgt ctgcgacgaa tgttattctt ggaagagaaa
      241 ttcacttttt tcactaacaa cattgcttta caattaaaaa caagtaaaaa agaaaataga
      301 ggaatgatac aaatggcaca acgcagaccg gcaggcaaaa aaataccttt tcaaaaagac
      361 tcattcttac aacaatttga gaaacttgcg caatcccgga aacatcatgt acttctcgaa
      421 agtgcaagag gcggcagata tagtatagcc ggtcttgatc caattgcgac tgtgaaagga
      481 aaagacggaa taactacaat taagcatggt gatgagatgc tgtttaaaga aggtgatcca
      541 ttacgggcct tccacagctg gtttaaaaca ctggaaacag aaacgaatca tgagttccct
      601 gactttcaag gcggggcaat cgggtttctc agctatgatt acgcacggta cattgaaaat
      661 tttaaaatgc tctcattaga tgatttagaa acaccagata tttattttct tgtttttgat
      721 gatatagcag tttatgacca tcaagaagag tctctatggc tgattactca tgttaatggt
      781 tctgatcagg aaacagcgga tgtgaagcta tctgagttag agcagatgtg gttgactgag
      841 cttcccgctg tcacttcgcg agagatgaag cctgaaacag ctggttcttt cgcggcgcca
      901 tttaccgagg atgggttctc acaagctgta gagaaaatca aacaatacat tgccagcgga
      961 gatgtgtttc aagtcaatct atcaataagg cagtcacagt cactgtctgt ccacccatat
     1021 caaatttaca aaaccttgag agaagtaaat ccttctcctt atatggcgta tttagaaaca
     1081 cctgatttcc aaatcatttg cggatcgcct gaactgcttg tcagcaaaaa gggcaagcta
     1141 ttagagacga gaccgattgc gggcacccgt tccagaggga aaacaaatga agaagacgag
     1201 gcgcttgcaa acgaattgat acacaatgaa aaagaacgcg cggaacatgt catgctggtt
     1261 gatcttgagc gaaatgatct gggaagagta tcacgttacg ggtctgtgcg cgtaaatgaa
     1321 ttcatggcaa ttgaaaaata ctcgcatgtg atgcacattg tgtctaatgt ccaaggtgaa
     1381 ctgcaggatg ggtatgatgc tgtagatatt attcatgctg tgtttcccgg aggaaccatt
     1441 actggtgcac cgaaagtaag aacgatggaa attatagaag aacttgagcc gacacgccga
     1501 gggctttata ctggatctat aggatggttt ggatataatc acgatctgca gtttaatatc
     1561 gtcattcgaa ccatttatgc aaccggaggg caggcattta tgcagtccgg tgcaggagtt
     1621 gtgattgatt ctgttccgaa gcacgaatac aaggaatcat tcaaaaaagc ttttgcgatg
     1681 caaagagcat tagagctgag cgaagaagag acaaaaatta gatgaggtga gcggagaaat
     1741 gattttaatg attgataact acgattcatt cacgtacaac ttggtacagt atttgggcga
     1801 gcttggggaa gagctggttg tgaaacgcaa tgacagcatc acaatcgatg aaattgaaga
     1861 actgtctccg gactttctga tgatatctcc cggaccgtgc agccctgatg aggcgggaat
     1921 cagcctcgaa gcaattaaac atttcgcagg gaaaattcct attttcggtg tatgtctcgg
     1981 acatcagtcc atcgcacaag tgttcggtgg tgatgttgtt agggcagaac ggcttatgca
     2041 cgggaaaacc tcggatatcg agcatgacgg caaaaccatt tttgaagggt tgaaaaatcc
     2101 ccttgttgcg acgcgatacc actcgctgat cgtaaaacct gagacgctgc caagctgttt
     2161 tacagtaaca gcacaaacga aagaaggaga aatcatggct attcgccaca atgacctccc
     2221 gatagagggt gtgcaatttc acccagagtc tattatgacc tcctttggga aagaaatgct
     2281 cagaaatttt attgagacat atcgcaagga agttattgcg tgatgatata tgtgaacggc
     2341 cggtatatgg aggagaaaga tgcagttctt tctccttttg accatgggtt tttatatggg
     2401 atcggtgttt tcgaaacctt caggctctac gaaggctgcc cgtttttgct tgattggcat
     2461 atagagaggc ttgaacgcgc gcttaaggat ctgcaaatcg aatatactgt ttcaaagcat
     2521 gagatccttg agatgctgga caagctgctt aagctaaatg atatcaagga tggcaatgcc
     2581 cgcgtcagac tgaacatatc agcgggaatc agcgataaag gctttgtcgc ccagacgtac
     2641 gataagccca ccgttttatg ctttgtgaat cagctgaaac cagaaagtct tcctttgcaa
     2701 aaagaaggaa aggttttatc gatacggaga aacactccag agggatcgtt ccggttaaaa
     2761 tctcatcatt atctaaataa tatgtatgcg aaacgggaaa ttggaaatga tccgcgtgtc
     2821 gagggaattt tcttaacgga ggatggagct gttgcagagg gtatcatatc taatgtcttc
     2881 tggagaaaag gtcgctgtat ctatacgcca tcgcttgata ccggtattct ggacggcgtc
     2941 acgagacgtt ttatcataga aaatgcaaag gatatcggcc tggagctcaa aacgggccga
     3001 tatgagctag aagctctctt gacagctgac gaagcgtgga tgacgaactc ggttcttgaa
     3061 atcatcccgt ttactaaaat agaagaagta aactatggaa gccaaagcgg agaagcaacc
     3121 tccgctcttc aattgttata caaaaaagaa ataaagaaca tgattcatga aaagggagga
     3181 agagcatggc gcagcacaca atagatcaaa cacaagtaat ccacactaag cccagcgctt
     3241 tatcatataa agagaagacg ctggtgatgg gaattttaaa cgtaacgcct gactctttct
     3301 cggacggcgg aaaatatgac agcttggaca aggcgctgct gcacgcgaaa gagatgatcg
     3361 atgatggtgc ccatatcatt gatattggag gggaatcgac aaggcctggc gctgagtgcg
     3421 tatctgagga tgaggagatg tccagagtca ttccggtgat tgagcggatt acgaaagagc
     3481 ttggtgttcc tatttctgta gacacgtaca aggcttctgt cgcagatgaa gcagtgaaag
     3541 ccggtgcatc cattatcaat gatatttggg gagccaaaca tgatccgaag atggcttccg
     3601 ttgcagctga acataatgtt ccaattgtac tcatgcataa ccgccctgaa agaaactaca
     3661 atgacttatt gccggatatg ctgtcggact taatggagag tgtaaaaatt gctgttgagg
     3721 ccggagtaga cgagaagaac attattcttg atcctggtat cggtttcccg aaaacctatc
     3781 acgataactt ggcagtgatg aacaaactag agattttcag cggattggga tatccggttc
     3841 ttctggcaac ctcccgaaaa agattcatcg gacgtgttct ggatcttccg cctgaggagc
     3901 gggctgaggg cacaggcgcg actgtgtgtc tcggcattca aaaaggctgt gacattgtca
     3961 gggtccatga tgtaaagcaa attgccagaa tggcgaaaat gatggacgcg atgctgaata
     4021 agggaggggt gcaccatgga taaagtttat gtagaaggta tggagtttta cggatatcac
     4081 ggtgtgttca cagaagaaaa caaacttggc cagcggttta aagtcgattt aaccgctgag
     4141 ctggatttaa gcaaagctgg acagacagac gaccttgagc aaacgatcaa ctatgctgag
     4201 ctctatcacg tatgtaaaga tatcgtggaa ggggagcctg tgaaattggt ggaaacgctg
     4261 gcggaacgta ttgctggcac tgttctcgga aaatttcagc ctgttcagca atgtacggtg
     4321 aaagtgatta agccagaccc gccaattccc ggacactata aatcagtagc aattgaaatt
     4381 acgagaaaaa agtcatgaac aacatagctt atattgcact tggatctaat attggagata
     4441 gagaaacgta tttaaggcaa gcagtggctt tactgcatca gcatgctgcg gtgacagtca
     4501 ctaaagtgtc gtctatttac gaaactgacc cggtcggata cgaagatcaa gctcaatttt
     4561 tgaatatggc tgttgaaatc aagacatcat tgaacccttt tgaactcctt gaactgacgc
     4621 agcagataga aaatgaatta ggcagaacaa gggaagtaag atggggaccg cggacggcag
     4681 accttgacat tttgttattt aatcgtgaaa atattgaaac agagcaacta attgttccgc
     4741 atccgagaat gtatgagcgt ttgtttgtcc ttgcgccgct tgcggaaatt tgccagcagg
     4801 ttgaaaaaga ggctacaagc gccgaaacag accaagaagg tgtaagagta tggaagcaga
     4861 aatctggggt agacgaattc
//