From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Rattus norvegicus MAP kinase kinase mRNA, complete cds.
Date: 21 Dec 92 20:51:47 GMT
Distribution: bionet
Checksum: 61062 6

LOCUS       RATMAPKK     2090 bp ss-mRNA            ROD       20-NOV-1992
DEFINITION  Rattus norvegicus MAP kinase kinase mRNA, complete cds.
ACCESSION   L04485
KEYWORDS    MAP kinase kinase; protein kinase.
SOURCE      Rattus norvegicus (strain Sprague-Dawley) (library: lambda gt10)
            kidney cDNA to mRNA.
  ORGANISM  Rattus norvegicus
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Rodentia; Myomorpha; Muridae; Murinae.
REFERENCE   1  (bases 1 to 2090)
  AUTHORS   Wu,J., Michel,H., Rossomando,A., Haystead,T.A., Shabanowitz,J.,
            Hunt,D.F. and Sturgill,T.W.
  TITLE     Renaturation and partial peptide sequencing of mitogen-activated
            protein kinase (MAP kinase) activator from rabbit skeletal muscle
  JOURNAL   Biochem. J. 285, 701-705 (1992)
  STANDARD  full automatic
REFERENCE   2  (bases 1 to 2090)
  AUTHORS   Wu,J., Harrison,J.K., Vincent,L.A., Haystead,C., Haystead,T.A.,
            Michel,H., Hunt,D.F., Lynch,K.R. and Sturgill,T.W.
  TITLE     Molecular structure of the protein-tyrosine/threonine kinase
            activating p42 mitogen-activated protein (MAP) kinase: MAP kinase
            kinase
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. (1992) In press
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     mRNA            1..2090
     mat_peptide     16..1191
                     /function="Protein tyrosine/threonine kinase specific for
                     MAP kinase"
                     /product="MAP kinase kinase"
                     /note="putative"
                     /codon_start=1
     misc_signal     133..177
                     /function="Encode a bipartite nuclear targeting sequence"
                     /note="putative"
     polyA_signal    2066..2071
     polyA_site      2090
                     /evidence="EXPERIMENTAL"
     CDS             13..1194
                     /function="Dual specificity protein tyrosine/threonine
                     kinase"
                     /standard_name="MAP kinase kinase"
                     /note="putative"
                     /product="protein kinase"
                     /codon_start=1
                     /translation="MPKKKPTPIQLNPAPDGSAVNGTSSAETNLEALQKKLEELELDE
                     QQRKRLEAFLTQKQKVGELKDDDFEKISELGAGNGGVVFKVSHKPSGLVMARKLIHLE
                     IKPAIRNQIIRELQVLHECNSPYIVGFYGAFYSDGEISICMEHMDGGSLDQVLKKAGR
                     IPEQILGKVSIAVIKGLTYLREKHKIMHRDVKPSNILVNSRGEIKLCDFGVSGQLIDS
                     MANSFVGTRSYMSPERLQGTHYSVQSDIWSMGLSLVEMAVGRYPIPPPDAKELELLFG
                     CQVEGDAAETPPRPRTPGRPLSSYGMDSRPPMAIFELLDYIVNEPPPKLPSGVFSLEF
                     QDFVNKCLIKNPAERADLKQLMVHAFIKRSDAEEVDFAGWLCSTIGLNQPSTPTHAAS
                     I"
BASE COUNT      525 a    480 c    535 g    550 t
ORIGIN      
        1 cccggctgca agatgcccaa gaagaagccg acgcccatcc agctgaaccc ggcccccgat
       61 ggctccgcgg ttaacgggac cagctcggcc gagaccaacc tggaggcctt gcagaagaag
      121 ctggaggagc tggagctgga cgagcagcag cggaagcgcc ttgaggcctt tctgacgcag
      181 aagcagaagg tgggagagtt gaaggatgat gactttgaga agatcagtga actgggggct
      241 ggcaatggtg gagtggtgtt caaggtctcc cacaagccat ctggcctggt tatggctagg
      301 aagctaattc acctggagat caaacccgca atccggaacc agatcatccg ggagctgcag
      361 gtgctgcatg agtgcaactc cccgtacata gtgggcttct acggggcctt ctacagtgac
      421 ggcgagatca gcatctgcat ggagcacatg gatggtgggt ccttggatca agtgctgaag
      481 aaagctggaa gaattcctga gcaaatttta ggaaaagtca gcatcgctgt gataaaaggc
      541 ctgacatatc tacgagagaa gcacaagatt atgcacagag atgtcaagcc ttccaacatt
      601 ctagtgaact cacgtgggga gatcaaactc tgcgattttg gggtcagcgg gcagctaatt
      661 gactccatgg ccaactcctt cgtgggaaca aggtcctaca tgtcgcctga gagactccag
      721 gggactcact actctgtgca gtcggacatc tggagcatgg ggctctctct ggtggagatg
      781 gcagttggaa gataccccat tcctcctcct gatgccaagg agctggagct gctgtttgga
      841 tgccaggtgg aaggagacgc ggccgaaacg ccacccaggc caaggacccc tgggaggccc
      901 ctcagctcat atggaatgga tagccgacct cccatggcaa tttttgagtt gttggattac
      961 atcgtcaatg agcctcctcc aaaactgccc agtggagtat tcagtctgga atttcaggat
     1021 tttgtgaata agtgcttaat aaagaaccct gcagagagag cagatctgaa gcagctcatg
     1081 gtacatgctt tcatcaagag atctgatgcc gaggaggtag acttcgcagg ctggctctgc
     1141 tccaccattg ggcttaacca gcccagcaca ccaacccacg ctgccagcat ctgagccttt
     1201 gggaagcagc agagaggaat cctctgccca gtggcatgcc atgttgcttt caggcctctc
     1261 ccatgcttgt ctatgttcag atgtgcatct catctgtgac aaaggatgaa gaacacagca
     1321 tgtgccaaat cgtacttgtg tcatttttaa tattgtcttt atcgctatgg ttactcccct
     1381 aagtggattg gctttgtgct tggggctatt tgtctgttca tcaaatacat gccaggttga
     1441 actacagtga aaccctggtg acctgggtgg tcttcttact gatgtttgcg ctgctgttca
     1501 tcgtgactca ctagctggct gcctgtattg tcaggattct cggacccttg gtacttcact
     1561 cttgctggtg acctctcagt ctgaggagag ggggccttct gagacccttc acaggcagtg
     1621 catgcatgaa aagcatgctt tgctgctact gaaatgagca ccagaacgtg tacatcatgg
     1681 tattttattt ttgcttttgg tatagaactc agcagttccc atttaaaaaa aaaatctaac
     1741 cagagcccat cactgccatg atagctgggg cttcagtctg tctactgtgg tgattttaga
     1801 cttctggttg tatttctata tttattttta aatatactgt gtgggatact tagtggtata
     1861 tgtctctgag tttggattag tgtttctaaa ttggtagtta ttttgaatgt cacaaatgga
     1921 ttaaggaatc aacgtatcaa gagttctatc tttcttccag tctaagtacc aatgctattg
     1981 taaacgtgta tagtgcctac aaattgtatg aaaacccttt taaccacttt actcaagatg
     2041 tttatcaaat ctaatctctt attctaataa aaatactatc aagttaaagt
//