From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: V.cholerae tcp gene cluster for biosynthesis of the TCP pilus
Date: 4 Sep 92 10:07:22 GMT
Distribution: bionet
Checksum: 25969    28

LOCUS       VIBTCP      13824 bp ds-DNA             BCT       04-SEP-1992
DEFINITION  V.cholerae tcp gene cluster for biosynthesis of the TCP pilus
ACCESSION   X64098
KEYWORDS    TCP gene cluster; gene cluster; lipoprotein; pilus; tcpA gene;
            tcpB gene; tcpC gene; tcpD gene; tcpE gene; tcpF gene; tcpH gene;
            tcpI gene; tcpJ gene; tcpM gene; tcpN gene; tcpO gene; tcpX gene;
            tcpY gene; tcpZ gene.
SOURCE      Vibrio cholerae DNA.
  ORGANISM  Vibrio cholerae
            Prokaryotae; Facultatively anaerobic gram-negative rods;
            Vibrionaceae.
REFERENCE   1  (bases 1 to 13824)
  AUTHORS   Ogierman,M.A. and Manning,P.A.
  TITLE     TCP pilus biosynthesis genes in Vibrio cholerae 01: gene sequence
            of tcpC encoding an outer membrane lipoprotein
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
REFERENCE   2  (bases 1 to 13824)
  AUTHORS   Manning,P.A.
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
REFERENCE   3  (bases 1 to 13824)
  AUTHORS   Ogierman,M.A. and Manning,P.A.
  TITLE     Genetic organization and nucleotide sequence of the promoter distal
            region of the tcp gene cluster Vibrio cholerae 01
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
REFERENCE   4  (bases 1 to 13824)
  AUTHORS   Ogierman,M.A., Meaney,C., Faast,R., Voos,E., Attridge,S.R. and
            Manning,P.A.
  TITLE     Comparison of the tcp regions of classical strain Z17561 and El Tor
            strain 017 of Vibrio cholerae 01
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
REFERENCE   5  (bases 1 to 13824)
  AUTHORS   Ogierman,M.A. and Manning,P.A.
  TITLE     homology of TepN, a putative regulatory protein of Vibrio cholerae,
            to the AraC family of transcriptional activators
  JOURNAL   Gene 116, 93-97 (1992)
  STANDARD  full automatic
COMMENT     *source: strain=ssp. classical 01, Z17561; *source: cell_line=
            E.coli K12 cosmid clone; *source: clone_library=cosmid; *source:
            clone=pPM2114;
            See also M33514 & M74708
            From EMBL    entry VCTCP;  dated 31-JUL-1992.
FEATURES             Location/Qualifiers
     CDS             complement(64..1284)
                     /gene="tcpI"
                     /codon_start=1
                     /translation="MIKKIISVFLLLACIITSAFTAFFYHSKLSDQTKSISSLSSQQA
                     QERLQSYQDSLDFYKKLNISLSVAIANSLRDKAVEELNAIALRIQENHGFIGVTFASL
                     DGTMFTDIGTLDWNAKTLRRDWFVKTVELGTKHYTAFDIDKTTGQHVLTIATPVYVGN
                     DIVGSVALDIAGDQIASPNGSGMFMMTDRNFNVFASDLTHSTLIGKDLTKEKPLFKNL
                     VSGQYVTFSDADSHWFAVSQTEIDGENKLFTIIDIQQIVQTYKRDIQLIIAGFSGFSC
                     VMLIGLYWVLSKELSGVRQIREWILSLSDGQIKERRPIKFHNELDTIAQSLENLQFRL
                     LDVVRNSHRTMNDLSIKQTDITYSIEGNTNNSQQELGLIEQVATATTQLSCTSFDVMQ
                     QAQSAELNARRPKN"
     prim_transcript 1437..>2808
     CDS             1721..2386
                     /gene="tcpH"
                     /codon_start=1
                     /translation="MGYVRVIYQFPDNLWWNECSNQVYYAQDPMKPERLIGTPSIMQA
                     KLLKILCEYHPSPCPNDQIIKALWPHGFISSESLTQAIKRTRDFLNDEHKTLIENVKL
                     QGYRINIIQVIVSENIVDEADCSQKKSVKERIKIEWGKINVVPYLVFSALYVALLPVI
                     WWSYGQWYQHELAGITHDLRDLARLPGITIQKLSEQKLMFAIDQHQCSVNYEQKTLEC
                     TKN"
     CDS             2371..2808
                     /gene="tcpX"
                     /codon_start=1
                     /translation="MHKKLKAWGGAAGLFVVALGVTLIALPMRQKNSHGTMIIDGTVT
                     QIFSTYQGNLSNVWLTQTDPQGNVVKSWTTRYQTLPDPSSQKLNLIPDYSESNVSRDY
                     NVLSIYQLGKGCFLAFPYKQLTAEKMLVFLSKRFLGSYHEPPR"
     prim_transcript 3256..>4005
     sig_peptide     3331..3405
     CDS             3331..4005
                     /gene="tcpA (pilin)"
                     /codon_start=1
                     /translation="MQLLKQLFKKKFVKEEHDKKTGQEGMTLLEVIIVLGIMGVVSAG
                     VVTLAQRAIDSQNMTKAAQSLNSIQVALTQTYRGLGNYPATADATAASKLTSGLVSLG
                     KISSDEAKNPFIGTNMNIFSFPRNAAANKAFAISVDGLTQAQCKTLITSVGDMFPYIA
                     IKAGGAVALADLGDFENSAAAAETGVGVIKSIAPASKNLDLTNITHVEKLCKGTAPFG
                     VAFGNS"
     mat_peptide     3406..4002
                     /gene="tcpA"
     terminator      4037..4060
     CDS             4086..5378
                     /gene="tcpB"
                     /codon_start=1
                     /translation="MRKYQQGVGLLEAILASAVLGMALVAAGSYYKREAELMIKSSNA
                     FDVIELSSQIQRYASLSKINNRTNPILKDNKAKEFKDADLKWLKLENCPTAGDVPTTG
                     NNNDLQDQFIACDADYRKGDLSYFGSQFEFSTYVHPSNPEIQRQIKQVVSYFQYRGME
                     RAFIGDAAGYVISEAKKKGFSAQDYRIVLIEPDRVGYFESNAISYEEFIENPSARENF
                     LLKATKDRTLALAVSLAQTGEIAMQRDGSVAFLEDSELCWDTAAGSANACLSVRYDTV
                     GNKTELDLKQIDVVSAKGLSFESDGKTKTPVVSTYETFQDGGRAKTINAIECPTGLNN
                     RFAAVVSSFSTAGQNANFSSESAKDSQGTTQKDGSKGPHALLSGISLNWTLTNKVWDV
                     TASIGIESGILPTSGIDSGSLLRNPKSLSFIAFQWCEN"
     CDS             5378..5830
                     /gene="tcpY"
                     /codon_start=1
                     /translation="MKKNYYIGACIALILSGPTFAQGEMHKTNEQIAEQSSVDLIAEI
                     YHSDRYAETFIESEEFEQEESPSKDPILFVTPYDQLKDKLESWADLHGYVVKWNTQKT
                     VQFDNAVAYEGDFEQVLTELASDINQIGIDINFKIFQKNKVIVVYSVR"
     sig_peptide     5833..5880
     CDS             5833..7302
                     /gene="tcpC (lipoprotein)"
                     /codon_start=1
                     /translation="MKKTIISTLVIGLVAGCSNTNLLKDNLASEQNVINLSKSSNERK
                     SRNIEFLSGAYLSERKVPKHDIKFSGKYVEFESKSPIELIDVLDGLSKQYNIQYVFSD
                     ELEDEKSEENKKSSGSSSAKKIKYSGPLAGFFDYLSSAYNMHFEFGHNNLVKAYHYKN
                     QVFNLQQYFDDNKFSSSMQIGGTSGTSSGLKGTADTAIESNSWKKIDEFLSASLGETG
                     KFTIFEDYSLVTVKARPDKFLLLHTFFDKLINESKMQIAVDYRVVSLSEERLNQLAAK
                     FGIENAGKYSITSDMVDAISLSQVGGGLGASYRSASARLDAVVNELSQEVMHEGHFIG
                     IPNRVMPLNVTTNSKYISSIETTKDTNTDEETRTVKVSDLVTGFSMMVMPKILDDGRI
                     QISSGFSRKQLVSIGTAQGITLPTVDENESMNTVTMNPGEVRLAMLFKDNYIQNSNGV
                     QLLGGGTENKKSARYIAVLVGASSYKTNDLASNRVNIYD"
     mat_peptide     5881..7299
                     /gene="tcpC"
     CDS             7295..7750
                     /gene="tcpD"
                     /codon_start=1
                     /translation="MTSIWLHESDFRYVNLDVERYQKKYRLTLTNGNKYVFIKDKEDI
                     SDVNPFIPYILMEEGMNNVLVKSDDYKDILIYQGVNIQCLDFLNDNDISVEKLVDFET
                     VELKADELNKIKARRLDAQLIEDEVKNNKVFIIGFIAIVIISIGVFWLM"
     CDS             7753..8574
                     /gene="tcpZ"
                     /codon_start=1
                     /translation="MKISSLKKGSNFSINIKNIKLDKKLLVAIIFLVLSILGGGAYLY
                     YENEKTKKLEQARLQKIQKENSDKQTYLSAFKSAFEGLDYQALTGFYDVLRSDIDFFR
                     VNNWLLDVMDCNVNCNLAFKRGSFDTFTYLEMNRNGAVIKPQFDQNKLQFANVDYISG
                     FRSIYLKDLTEQERDKSENIIEQCSTKLSELYNLQLLMKEQVKFKINLPRNVTSISGY
                     DWVKNSDIKFGSIEIENMPEKNLGLMKNIMNNSMMITSISLQNSSFKSKLNYYCY"
     CDS             8590..9048
                     /gene="tcpE"
                     /codon_start=1
                     /translation="MNIKLSFISIAFLSLSFNVAANEFEKSQEHYKSVTDLKNKIEIL
                     ELEKKITELSGEIRNARMPKIDKSAPVLSPQPVVKSSEELQKSIEHIEEELKVELAYL
                     VNNGQQKKYTFNLNGKLITLVNGDFVNGWKFIEDQNKIQFFKGNKVIDVN"
     CDS             9038..10549
                     /gene="tcpF"
                     /codon_start=1
                     /translation="MSIDIKYLSRIDIDREEFFFKDSRLMCKKFDEEREVLTLLEFDT
                     KFRVNLLKKDKVYKYFLVSDANHKLLIANLVTEQQAKDLSFIEKDIMKIASSATAYGA
                     SDIHFIREDRICKIKFRVNGTMIDYREILSSEADSLMFVLYNVMATTKETTWNRKLPQ
                     DANIILVINEKAYRFRYAHMPLFGEGGKNYHAVVRIIYPSNNFVCTNYQDIGYNEADT
                     DAIARILNTSYGLFIVSGTTGSGKSTSLKKYIELLFFNKYKGKGCFVTVEDPVEYLIS
                     GAQQSSIVADNDDKTKNPFADAVRSAMRRDPDVIMIGEIRDKPTVEALSSAVESGHYC
                     LTTIHAGSVVSVLQRLSGLGMKADKIASPGFLAGITSQKLIPELCPSCKVSFVDERYQ
                     RAVFSANENGCEACNHSGFKGRLLLLETLVPTVEDLELVASENWVSLYRKYRERRFIK
                     TGKKGLGEGFSIKDKAYYNVLKGKVCHEYFMLHFGQLDHEDENIIYENYLQEV"
     CDS             10566..11546
                     /gene="tcpM"
                     /codon_start=1
                     /translation="MLVDLLNDNIPLYDALNKIQNEGVGIYDKNFIKSIELIKDRMKS
                     NSSLTDALTGLIPDKEVLMINVAENSGKISSGIAAIRKNIIDADEIKSKAISSMITPS
                     VMLIVTMVVIAGYSVKVFPTFESVLPVSRWPGVTQALYNLGFSLYEGLWIKVLIFVAI
                     FITILVFMSKNITGNFRDGFLDKLPPFNFVKHIAATEFLANMSMLLDSRVPFKEGLDI
                     VDHKTTRWLSSHLQRMKANMQEGLDYKQALDTNLLDKKMLLTMAVYSELPNFSDVMQK
                     LAIEANINLHKKIATLAGVMKNISLITLALSVIWIFGAIFSLVDKLSSSL"
     CDS             11556..12572
                     /gene="tcpO"
                     /codon_start=1
                     /translation="MRYKKTLMLSIMITSFNSFAINENYSSTSTVYATSNEATESRGS
                     EHLRYPYLECIKIGMSRDYLENCVKVSFPTSQDMFYDAYPSTESDGAKTRTKEDFSAR
                     LLAGDYDSLQKLYIDFYLAQTTFDWEIPTRDQIETLVNYANEGKLSTALNQEYITGRF
                     LTKENGRYDIVNVGGVPDNTPVKLPAIVSKRGLMGTTSVVNAIPNEIYPHIKVYEGTL
                     SRLKPGGAMIAVLEYDVNELSKHGYTNLWDVQFKVLVGVPHAETGVIYDPVYEETVKP
                     YQPSNNLTGKKLYNVSTNDMHNGYKWSNTMFSNSNYKTQILLTKGDGSGVKLYSKAYS
                     ENFK"
     terminator      12576..12615
     CDS             12779..13609
                     /gene="tcpN"
                     /codon_start=1
                     /translation="MIGKKSFQTNVYRMSKFDTYIFNNLYINDYKMFWIDSGIAKLID
                     KNCLVSYEINSSSIILLKKNSIQRFSLTSLSDENINVSVITISDSFIRSLKSYILGDL
                     MIRNLYSENKDLLLWNCEHNDIAVLSEVVNGFREINYSDEFLKVFFSGFFSKVEKKYN
                     SIFITDDLDAMEKISCLVKSDITRNWRWADICGELRTNRMILKKELESRGVKFRELIN
                     SIRISYSISLMKTGEFKIKQIAYQSGFASVSNFSTVFKSTMNVAPSEYLFMLTGVAEK
                     "
     CDS             13609..>13824
                     /gene="tcpJ"
                     /codon_start=1
                     /translation="MEYVYLILFSIVSLILGSFSNVVIYRLPRKILLKNHFFYDIDSN
                     RSMCPKCGNKISWYDNVPLLSYLLLHGN"
BASE COUNT     4625 a   2158 c   2727 g   4314 t
ORIGIN
        1 ggtcagatgg gttacatttc gtttgtttgc ttgatgaatc gataatatca tgactttcag
       61 caatcaattt ttgggccgtc tcgcattaag ctctgcactt tgagcttgtt gcattacatc
      121 aaaagaagta catgatagtt gcgttgttgc tgtggccact tgttcaatga gcccaagttc
      181 ttgctgtgaa ttattcgtat tcccttcaat tgaatacgta atgtcagtct gtttgatact
      241 taaatcattc atagtccgat gagaatttct caccacatca agaagcctaa attgtaaatt
      301 ttcaagactt tgggcaatag tgtcgagctc gttatggaat tttataggcc tgcgttcttt
      361 tatctgacca tcagaaaggg aaagaatcca ttctctgatc tgtctaacac ctgagagttc
      421 tttgcttaga acccaatata gtccaattag catcacgcat gaaaaaccac taaatccagc
      481 aataatcaac tggatatctc gcttgtatgt ttggacgatt tgttgaatat caataatagt
      541 gaacagttta ttttcaccat ctatttctgt ttgtgacact gcaaaccaat gactatcagc
      601 atcactgaag gtgacatact ggcctgagac cagatttttg aacagaggct tctctttcgt
      661 taaatctttt ccaattaagg tactgtgagt gagatcagat gcaaatacat taaaattcct
      721 atccgtcatc ataaacatcc cactgccgtt aggtgaggca atctgatcac ctgcaatatc
      781 taaagcgaca ctgccgacaa tgtcgttgcc tacataaaca ggggtggcaa tagtgagaac
      841 gtgttgccca gttgttttat cgatatcaaa agctgtgtaa tgtttagtac cgagttcgac
      901 cgttttaaca aaccaatctc ttctcaatgt ctttgcattc caatctagag taccaatatc
      961 tgtaaacatg gtgccatcta aactagcgaa ggtaacacca ataaatccat gattttcctg
     1021 tatacgtaaa gcaatagcgt tgagttcttc gactgcttta tcgcgaagtg aattggctat
     1081 tgcaactgag agagagatat taagtttttt ataaaaatct aaagaatcct gataactttg
     1141 caaccgttct tgtgcctgct gagaactaag gctagatata gattttgttt gatctgatag
     1201 tttcgagtga tagaaaaagg cagtaaaagc cgacgtaatg atgcacgcaa gtaataaaaa
     1261 aaccgaaatt atttttttta tcattagttt aactctaagt ttaaatggtt atcacggagt
     1321 acttcgtgat aattaaaaat aaaattataa aataatgatg tgaaaaatca gcttttatcg
     1381 ttttaaatag tatttttttt ctttaggaaa ataaacttat aaagttaaaa aaaagcccca
     1441 aacggaaggg gcaaagtgtc acaggaaaga taatgtaacc aagttaatag atatggaata
     1501 ggcactatag ggggagtgct aaaccgaatg aactgtaacg aatattgctt tccgatagca
     1561 tggtttgctg ttttttttaa tgttatttta ttttttttaa caatttaaat attatgcaat
     1621 cgagttctca ttatcaactg caaaattaga ttgcaaataa ttatattaaa aaaaatagac
     1681 ataaaaaaat gggtcgttat gattaagaaa atgtaaagta atggggtatg tccgcgtgat
     1741 ttatcaattt cccgataacc tttggtggaa cgaatgcagt aatcaagtct attatgcaca
     1801 agatccaatg aagccggaaa ggctgattgg tacaccgagc ataatgcagg ctaagttatt
     1861 gaaaatactc tgtgaatatc atccttcccc ctgtccaaat gatcaaataa ttaaagcact
     1921 ttggcctcat ggatttatca gctctgaaag tctaactcag gcaatcaaaa gaaccagaga
     1981 ttttttgaat gatgaacata agacgttgat cgaaaatgta aagttacaag gttatcgtat
     2041 taatattata caagttattg tttctgaaaa cattgttgat gaagctgact gtagtcaaaa
     2101 aaaatcagta aaagagcgga taaaaattga gtgggggaag ataaacgtag ttccttatct
     2161 tgttttttcg gcgctttatg ttgctttgct acctgtgatt tggtggagtt atggccaatg
     2221 gtatcaacat gaattagccg gcatcactca tgatctacga gatttagcaa ggttaccggg
     2281 gataacaatc caaaaactgt cagaacaaaa actcatgttc gctattgatc aacatcagtg
     2341 ttccgtgaat tatgaacaga agacattaga atgcacaaaa aattaaaagc ttgggggggg
     2401 gctgcaggtc tattcgttgt agcactagga gtaacgctca tcgcactccc gatgcgacaa
     2461 aaaaactcgc acggcacaat gattattgat ggtacagtca cacaaatttt ttctacttat
     2521 caaggtaatc tatccaatgt ttggcttacc cagacagatc cacaaggtaa cgtagtcaaa
     2581 agttggacta cacgttatca aacattgcca gatcctagct ctcagaagct aaatttgatt
     2641 cccgactact cagaaagtaa tgtgagccgt gattacaatg tgttgagtat ttatcaactc
     2701 ggcaaaggtt gttttctcgc cttcccttac aagcagctta cggctgaaaa aatgttggtt
     2761 ttcctgtcaa agcgattttt agggtcttat catgagccgc ctagatagtg tgtgacgagt
     2821 aggcgctcct ccacagtcaa agtgactgaa agtcatctct tcatctttac cctaatgttc
     2881 gcagttgtat tgagatcgtc aacaatataa caagtgtcaa ttaattggct atacagcatg
     2941 cacgagacga acactgtcag taccgcacca gatccacgta ggtgggtata gtgataagac
     3001 gtcttaccca atttttatcg tcattcataa tttcgatctc cactccggaa atattttaac
     3061 gcattttatt tgcattaaat tactttaaat catttgaatt gaataagttg gccttttttt
     3121 agtgtctgat ttattttgtg cgtgaatgtt actcgtgttt ctttcaatgc aagtgtgtta
     3181 ttaaaaaaat aaaaaaacac agcaaaaaac tgacatctgt caattgtagg tgactttgtg
     3241 tggttaaatg tgcgtgttgc ttacgttatc taaaaaagac caagcgacgc atttccttta
     3301 aagacagtaa aatggtggag ttacataaat atgcaattat taaaacagct ttttaagaag
     3361 aaatttgtaa aagaagaaca cgataagaaa accggtcaag agggtatgac attactcgaa
     3421 gtgatcatcg ttctaggcat tatgggggtg gtttcggcgg gggttgttac tctggcgcag
     3481 cgtgcgattg attcgcagaa tatgaccaag gccgcgcaaa gtctcaatag tatccaagtt
     3541 gcactgacac agacataccg tggtctaggt aattatccag caacagctga tgcgacagct
     3601 gctagtaagc taacttcagg cttggttagt ttaggtaaaa tatcatccga tgaggcaaaa
     3661 aacccattca ttggtacaaa tatgaatatt ttttcatttc cgcgtaatgc agcagctaat
     3721 aaagcatttg caatttcagt ggatggtctg acacaggctc aatgcaagac acttattacc
     3781 agtgtcggtg atatgttccc atatattgca atcaaagctg gtggcgcagt agcacttgca
     3841 gatctaggtg attttgagaa ttctgcagca gcggctgaga caggcgttgg tgtgatcaaa
     3901 tctatcgctc ccgctagtaa gaatttagat ctaacgaaca tcactcacgt tgagaaatta
     3961 tgtaaaggta ctgctccatt cggcgttgca tttggtaaca gctaattcaa ataagtttgt
     4021 ttaacttaat cttaacgttg cccattaaat aatgggcaac ttattaaatt caatgtggta
     4081 tcaatatgag aaaataccaa caaggtgtcg gattattgga ggcgattctg gcttctgccg
     4141 tattagggat ggcattggtc gctgctggga gctattacaa gcgggaagct gaactcatga
     4201 ttaaatccag taacgcattt gatgttattg agttgtcatc tcaaatccag cgttacgcct
     4261 ctttaagtaa aattaataat cgtactaacc caatactcaa agataataag gcaaaagaat
     4321 ttaaagatgc ggatttgaaa tggttaaagc ttgaaaactg ccccacagca ggtgatgtac
     4381 cgacaacggg taataataat gatttacaag atcagtttat tgcttgtgat gcagattata
     4441 ggaaaggtga tctaagctat tttgggagtc agtttgaatt ctcaacctat gtacatcctt
     4501 ccaacccgga aattcaacga caaataaaac aagtcgtcag ctattttcaa tatcgaggca
     4561 tggaaagagc gtttattggc gatgctgcgg gttatgttat cagtgaggcc aagaaaaaag
     4621 gatttagcgc acaagactat cgaatcgttc taatagaacc tgatcgtgtc gggtattttg
     4681 agtcaaatgc tattagctat gaagaattta tagaaaatcc atccgctcgc gaaaattttc
     4741 ttttaaaagc gacgaaagat agaactctgg cgttggcggt cagtcttgct caaaccggag
     4801 aaatagcaat gcaacgtgat ggttccgttg catttttaga agatagtgag ttgtgttggg
     4861 atacggcggc aggtagtgca aatgcttgtc tctcggttcg gtacgatacc gttggaaata
     4921 aaacagaact ggatttaaaa cagatagatg tggtcagtgc gaaaggccta tcatttgaaa
     4981 gtgatggtaa aacaaaaaca ccagtggttt cgacttatga gacttttcaa gatggtgggc
     5041 gggctaaaac cattaacgct attgaatgcc caacaggttt aaataatcgt tttgccgcgg
     5101 tggtgagttc attttcaact gctggacaga atgcaaactt tagttcagag agtgctaagg
     5161 attctcaagg cacaacccaa aaggatggta gcaaaggacc gcatgcgctt ctatccggta
     5221 tatcactgaa ctggacatta acgaataagg tatgggatgt aaccgcctcg attggtatcg
     5281 agtccggtat attgcctact tcaggtatcg attctggatc gttattacga aaccctaaat
     5341 cattgtcttt tatagcgttt caatggtgtg aaaattaatg aaaaaaaact attacatagg
     5401 tgcatgcatc gcgctcatat taagcgggcc aacctttgca caaggagaga tgcacaaaac
     5461 aaatgagcag attgcagaac agtcctcagt tgatttaatc gcagaaattt atcattcaga
     5521 tcgatatgca gaaacgttta ttgaatccga agagttcgaa caagaagaaa gcccgtcaaa
     5581 agacccaatc ttgtttgtga caccttatga tcaattaaaa gataaattgg aatcttgggc
     5641 tgatttacac ggttatgttg tgaaatggaa tacacagaaa acggttcagt tcgataatgc
     5701 tgtggcttat gaaggagact ttgaacaagt tttaactgag ttggctagcg atattaacca
     5761 aattggcata gacattaact ttaaaatatt tcaaaaaaat aaagttattg tcgtctattc
     5821 agtgagataa gtatgaaaaa aacaataatc agtacacttg ttatcggttt ggttgccggt
     5881 tgttctaata cgaacttgct aaaagacaat ctggctagcg agcaaaatgt catcaactta
     5941 agtaagtctt caaacgaacg caaatctaga aatattgaat ttctctctgg tgcctattta
     6001 agtgagagaa aagtgccgaa gcatgacatt aagttcagtg gtaagtatgt tgagtttgaa
     6061 agtaaatcac cgattgagtt aatcgatgtt cttgatggac tttctaagca atataatatt
     6121 cagtatgtat tctcagatga gttagaagat gaaaagtcgg aagagaataa aaagtcatca
     6181 ggctcatcat cggcgaagaa aataaaatac tcaggacctc tggctggttt ttttgattat
     6241 ttaagtagtg catataacat gcactttgaa tttggtcata ataacttagt taaggcatat
     6301 cattataaaa atcaagtttt taacctccag caatactttg atgataataa atttagctcg
     6361 tcaatgcaga ttgggggtac cagtggcaca tcaagtggtc ttaaaggtac tgcagatacc
     6421 gctatagaat ctaatagttg gaaaaaaatc gatgagtttt taagtgcatc cttaggtgaa
     6481 actggaaaat ttacaatttt tgaagattat tcactagtta cagtaaaagc ccgacctgat
     6541 aagtttttat tgttacatac tttctttgac aagttgatca atgagagcaa gatgcaaatc
     6601 gcggttgatt atagagtggt ttctcttagt gaagaacgct taaatcagtt agctgccaaa
     6661 tttggtattg aaaatgcagg gaaatacagt attaccagtg atatggtcga tgcgatctct
     6721 ttaagtcaag tagggggggg attaggcgct tcatatcgct ctgcctcagc aagattagat
     6781 gcagtggtta atgagttatc acaggaagta atgcatgagg ggcattttat cggtatccct
     6841 aacagagtaa tgccactaaa tgtcactaca aactcgaagt atatatcctc aatcgaaaca
     6901 acaaaagata ccaatactga tgaggaaacg agaactgtca aagtttctga cttagtaaca
     6961 ggttttagca tgatggttat gccgaaaatc ttagatgatg gacgaattca aatatcgtct
     7021 ggcttttcaa gaaaacagtt agtgtctatt ggtactgcac aaggtattac tctaccaaca
     7081 gttgatgaaa atgaatcaat gaataccgta acgatgaatc ctggtgaagt acgcctagca
     7141 atgctattta aggataatta cattcagaat agtaatggtg ttcaattatt aggtggtggt
     7201 actgaaaata agaaatcggc tcgttatatt gctgtgcttg ttggtgcaag cagttacaaa
     7261 accaatgatc ttgctagtaa tagagtaaat atatatgact agcatatggt tacatgagtc
     7321 agattttaga tatgttaact tagatgtaga aagataccaa aaaaaatatc gtttgacatt
     7381 gactaatgga aataagtacg tatttattaa agataaagaa gatatatctg atgttaatcc
     7441 atttatccct tatattctca tggaagaagg aatgaataat gtattagtaa aatcagatga
     7501 ttacaaagat atcctcattt atcaaggcgt caatattcaa tgtctggatt ttcttaatga
     7561 taatgatata tccgttgaga aattagttga ttttgaaaca gtagaactta aagcagatga
     7621 gctaaataaa ataaaagcac gaaggttgga tgcacaactg attgaagatg aagtcaaaaa
     7681 taataaagtg tttattattg gattcattgc tattgtaata attagtatcg gcgtattttg
     7741 gttaatgtga ttatgaagat tagcagtttg aagaagggaa gcaatttcag tattaatata
     7801 aaaaatataa agcttgataa aaaattgtta gtggcaatta tttttttagt tttatcaata
     7861 ctgggaggtg gagcctattt atactatgaa aatgagaaaa cgaaaaagtt agagcaggca
     7921 agattacaga aaattcaaaa agagaactct gacaaacaaa cctatttgtc agcttttaaa
     7981 tcggcttttg aaggtcttga ttaccaagcg ctaactggtt tttatgatgt actcagatct
     8041 gatatcgatt ttttcagggt aaataattgg ttattagatg taatggactg taatgttaac
     8101 tgtaatttag cctttaaacg aggaagcttt gacaccttta cttatttaga aatgaatcga
     8161 aatggagctg ttataaaacc acagttcgac cagaataagc tccagtttgc aaatgtggat
     8221 tatatatctg gttttcgttc tatttatctc aaagatctca ctgagcaaga gagagataag
     8281 agtgaaaata tcattgaaca gtgctcaaca aaactttctg aactgtataa tttgcagttg
     8341 ttaatgaaag aacaggtgaa atttaaaata aatcttccta ggaatgtaac ttcaatatcg
     8401 ggttatgact gggtaaaaaa tagtgacatt aaattcggtt ctattgaaat tgaaaatatg
     8461 ccagagaaaa acttgggtct gatgaaaaat attatgaata atagtatgat gattacgtct
     8521 atctctttgc aaaattcaag ttttaaatcc aagttaaatt attactgcta ttaaggtggg
     8581 attagagaaa tgaatatcaa actatcattt atttcaattg cttttctatc gttaagtttt
     8641 aatgttgctg ctaacgaatt tgaaaaatct caagagcact acaaaagtgt aactgattta
     8701 aaaaacaaaa tcgaaatact agagttagaa aagaaaataa cagagctgag tggcgagatt
     8761 cgcaatgcaa gaatgccaaa aatagataag tctgcacccg ttttatctcc tcagcctgta
     8821 gtaaaatcgt cagaagaatt acaaaaaagt atcgaacata tagaagaaga gctcaaagtg
     8881 gaattggctt atttggttaa caatggtcaa caaaaaaaat atacattcaa tcttaatggc
     8941 aaacttatca cattggttaa tggtgatttt gtaaatggtt ggaaattcat tgaagaccaa
     9001 aataaaattc aatttttcaa gggcaataag gtaatcgatg tcaattgata taaagtattt
     9061 aagtcgtatt gatatagatc gtgaggagtt tttctttaaa gactcgagat taatgtgtaa
     9121 gaaattcgat gaagagcgtg aggtattaac tcttctcgaa ttcgatacaa aatttcgggt
     9181 caatcttctg aagaaagata aagtttataa gtatttctta gtttcagatg caaatcataa
     9241 attattaata gcgaatcttg ttaccgagca gcaagctaaa gacttaagtt ttatagaaaa
     9301 agatataatg aaaattgcat catctgctac agcttatggt gcaagtgaca tccacttcat
     9361 ccgagaggat aggatttgta aaataaaatt ccgtgtgaat ggcacaatga tcgactatag
     9421 agaaatatta agttcagaag ccgattcgct aatgtttgtt ctatacaatg tgatggcaac
     9481 aacgaaagaa acgacgtgga accgaaaact acctcaagat gcaaatatta ttcttgtgat
     9541 caacgaaaag gcatatcgct ttcgttacgc acatatgcct ttatttggtg agggagggaa
     9601 aaactatcat gcggtagttc gtattatcta tccttcaaat aattttgttt gtacaaacta
     9661 tcaagatatt gggtataacg aggctgatac cgatgcgatt gcaagaattc taaatacatc
     9721 ttatggattg ttcattgttt ccggaacaac aggttctggt aaatcaacat ctttgaaaaa
     9781 gtatatagaa ttacttttct ttaataaata taagggaaaa ggatgttttg tcaccgttga
     9841 agatcctgtc gagtatttaa tttctggagc acagcaaagc tcaattgttg ccgataatga
     9901 tgataaaaca aagaacccat ttgctgatgc ggtacgttca gcgatgcgtc gagatccgga
     9961 cgttattatg atcggtgaaa ttcgtgataa acctaccgtt gaggctctat caagtgctgt
    10021 agaaagtggc cactattgtc ttacgaccat ccatgcaggt tctgttgttt ctgttttaca
    10081 aagactttca ggattaggga tgaaggctga caaaatagca tcacctggat ttctggctgg
    10141 tattacaagt cagaaattaa ttcccgaact ttgcccaagt tgtaaagttt cctttgttga
    10201 tgaacgttat cagcgtgctg tatttagcgc gaatgaaaat ggctgtgagg catgtaatca
    10261 ttctgggttt aaaggaaggc tgcttttatt agaaacatta gtcccaacag ttgaagattt
    10321 agagctagtg gcgagtgaaa actgggtttc cctttatcgt aaatatcggg agcgacgctt
    10381 tataaaaact ggaaaaaaag gtcttggtga aggcttcagc attaaagata aagcttatta
    10441 caatgtttta aaaggtaaag tgtgtcacga atattttatg ctgcatttcg gtcagttaga
    10501 tcatgaggat gaaaatataa tatatgaaaa ttatctccaa gaagtatagg ctagaacttt
    10561 attcaatgct tgtggactta ttgaatgata atatcccact ctatgatgca ttaaataaaa
    10621 tacaaaatga gggagtgggc atctatgata agaattttat taaatccatt gaattaatta
    10681 aggataggat gaaaagtaat tcatcactaa cggatgcctt aactggacta ataccagata
    10741 aagaagtttt gatgattaat gttgcagaaa actcaggtaa gatttcgagt ggtattgctg
    10801 caatacgcaa aaacattatc gatgcagatg agatcaaatc taaagctatc tcatctatga
    10861 tcacgcctag cgtgatgctt atcgtgacta tggttgttat tgctggctat tcagtaaaag
    10921 tatttcccac atttgagtct gtacttcctg tgagccggtg gcccggtgtt acccaagcac
    10981 tttataacct tggattttca ttgtatgaag gcttgtggat caaagttctt atttttgtcg
    11041 ctatcttcat tacgatttta gtttttatgt ctaaaaacat tacagggaat ttcagagatg
    11101 gttttttaga taagctgcca ccttttaatt ttgtaaagca tattgcagca acagaatttt
    11161 tggccaatat gtccatgttg ctagatagtc gagtcccatt taaagaaggt ctggacattg
    11221 tcgatcataa aacgacacgc tggctctcct cacatttgca aagaatgaaa gctaacatgc
    11281 aagaagggct agactacaaa caggctctcg acaccaattt actagataaa aaaatgctcc
    11341 tgacaatggc tgtttattca gaactaccta atttttcgga tgtaatgcaa aaattagcta
    11401 ttgaagccaa tataaatcta cataaaaaaa ttgccacttt agcaggggta atgaaaaata
    11461 tttcactaat aacgttggcg ttgtctgtga tttggatctt cggtgcgatc ttttcgctcg
    11521 ttgataaact aagttcatct ttatagtgag gatatatgag atataaaaaa accttaatgt
    11581 tatcaatcat gattacatca tttaactcat ttgcaattaa tgaaaattat agttcaacca
    11641 gtacggttta tgctacgtct aatgaagcta cggaatcaag ggggagtgag caccttagat
    11701 acccttattt agagtgtata aaaatcggta tgagtagaga ttatcttgaa aactgtgtga
    11761 aagtatcttt tccaacctct caagatatgt tttatgacgc atacccatcg acagaatctg
    11821 acggtgctaa gacaagaaca aaagaagatt tctctgctcg gcttttagct ggtgattatg
    11881 atagtttaca gaaattgtat atagattttt atcttgctca aacgactttc gattgggaaa
    11941 taccgacaag ggatcaaatt gagacactag ttaattatgc taatgaaggt aaattgtcta
    12001 cggcacttaa tcaagagtat attacaggtc gatttcttac aaaagaaaat ggtcgatatg
    12061 atattgtgaa tgttggtggt gttccagata atactccagt taaactacca gctattgttt
    12121 ctaaacgcgg actaatgggt acaacttctg tagttaatgc aattcccaat gaaatttatc
    12181 ctcatatcaa agtttatgaa gggactctta gtcgtttaaa acctggtggt gcaatgatcg
    12241 cagtattaga atatgacgta aatgaattaa gtaagcacgg gtataccaat ctgtgggatg
    12301 tgcagtttaa ggttcttgtt ggagttccac atgcagaaac aggagttatc tatgaccctg
    12361 tttatgaaga aactgtcaaa ccatatcagc ctagtaacaa cttaacgggt aagaagttgt
    12421 ataatgtatc tacaaatgac atgcataatg gttacaagtg gtcaaatact atgttctcaa
    12481 attctaatta taaaacgcaa atattattaa ctaaaggaga tggaagtggt gtgaaactgt
    12541 atagcaaagc atattcagag aactttaaat aaaatagata agataacagc cacattcgtg
    12601 gctgttatct tatctcaaaa aacataaaat aacatgagtt actttatgtt tttcttatgt
    12661 aatacgtctg taacttgttc ttatgtctgt tatgtagttt tatattaact tcgattttaa
    12721 gataacttta cgtggatggc tctctgcgtt tatttatata tcttcagagt agaacgcaat
    12781 gattgggaaa aaatcttttc aaactaatgt atatagaatg agtaaatttg atacttacat
    12841 attcaataac ttatatataa acgactacaa aatgttctgg atagatagtg gaattgcaaa
    12901 gcttatagat aaaaattgct tggttagtta tgagataaat tcaagttcaa ttatcttact
    12961 caagaaaaac tctattcagc gattttcttt gacttcatta tcagatgaaa atataaatgt
    13021 ttctgtaatt acaataagtg attcatttat acgttcacta aaatcttaca ttcttggtga
    13081 tctcatgata aggaatttat atagtgaaaa taaagatcta ttactttgga attgtgaaca
    13141 taatgatata gctgtccttt ctgaagtggt aaatggtttc agagaaatta attattcaga
    13201 tgagttccta aaagtttttt tttcagggtt cttctcgaaa gtagaaaaaa aatataactc
    13261 tatatttatt actgatgatc ttgatgctat ggagaaaatt tcatgtttag taaaaagtga
    13321 tattacgcgt aattggcgtt gggcagatat ttgtggtgaa ttaagaacga atcggatgat
    13381 tttgaaaaaa gaactagagt ctcgaggagt aaagtttaga gaattaatta atagcattcg
    13441 aatatcatat tcaatttcac taatgaaaac cggtgaattc aaaataaaac agattgcata
    13501 tcagtctggg tttgcaagcg tttcaaattt ttctacagta tttaagtcaa ccatgaatgt
    13561 agcaccaagt gaatatttat ttatgttgac aggagttgca gaaaaataat ggaatacgtt
    13621 tacttgatcc tattttcgat tgtatcactg attttgggta gttttagtaa tgttgtgatt
    13681 tatcgtctac caagaaaaat tttactaaag aatcattttt tttatgacat cgattcaaat
    13741 cgttccatgt gccctaaatg tggaaataaa atcagttggt atgataatgt tccattgtta
    13801 agttatttgt tacttcatgg taat
//