From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Tomato polygalacturonase isoenzyme 1 beta subunit mRNA, complete
Date: 31 Dec 92 01:41:19 GMT
Distribution: bionet
Checksum: 26096 5

LOCUS       TOMPOLBETA   2192 bp ss-mRNA            PLN       17-DEC-1992
DEFINITION  Tomato polygalacturonase isoenzyme 1 beta subunit mRNA, complete
            cds.
ACCESSION   M98466
KEYWORDS    polygalacturonase isoenzyme 1 beta subunit.
SOURCE      Lycopersicon esculentum (cultivar Ailsa Craig) 30 days after
            pollination tomato fruit cDNA to mRNA.
  ORGANISM  Lycopersicon esculentum
            Eukaryota; Plantae; Embryobionta; Magnoliophyta; Magnoliopsida;
            Asteridae; Solanales; Solanaceae.
REFERENCE   1  (bases 1 to 2192)
  AUTHORS   Zheng,L., Heupel,R.C. and DellaPenna,D.
  TITLE     The beta subunit of tomato fruit polygalacturonase isoenzyme 1:
            isolation, characterization, and identification of unique
            structural features
  JOURNAL   Plant Cell 4, 1147-1156 (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     sig_peptide     34..123
                     /codon_start=1
     mat_peptide     358..1224
                     /product="polygalacturonase isoenzyme 1 beta subunit"
                     /codon_start=1
     polyA_site      2192
     CDS             34..1926
                     /product="polygalacturonase isoenzyme 1 beta subunit"
                     /codon_start=1
                     /translation="MHTKIHLPPCILLLLLFSLPSFNVVVGGDGESGNPFTPKGYLIR
                     YWKKQISNDLPKPWFLLNKASPLNAAQYATYTKLVADQNALTTQLHTFCSSANLMCAP
                     DLSPSLEKHSGDIHFATYSDKNFTNYGTNEPGIGVNTFKNYSEGENIPVNSFRRYGRG
                     SPRDNKFDNYASDGNVIDQSFNSYSTSTAGGSGKFTNYAANANDPNLHFTSYSDQGTG
                     GVQKFTIYSQEANAGDQYFKSYGKNGNGANGEFVSYGNDTNVIGSTFTNYGQTANGGD
                     QKFTSYGFNGNVPENHFTNYGAGGNGPSETFNSYRDQSNVGDDTFTTYVKDANGGEAN
                     FTNYGQSFNEGTDVFTTYGKGGNDPHINFKTYGVNNTFKDYVKDTATFSNYHNKTSQV
                     LASLMEVNGGKKVNNRWVEPGKFFREKMLKSGTIMPMPDIKDKMPKRSFLPRVIASKL
                     PFSTSKIAELKKIFHAGDESQVEKMIGDALSECERAPSAGETKRCVNSAEDMIDFATS
                     VLGRNVVVRTTEDTKGSNGNIMIGSVKGINGGKVTKSVSCHQTLYPYLLYYCHSVPKV
                     RVYEADILDPNSKVKINHGVAICHVDTSSWGPSHGAFVALGSGPGKIEVCHWIFENDM
                     TWAIAD"
BASE COUNT      687 a    423 c    451 g    631 t
ORIGIN      
        1 tctctctctt catctctgtt tcacaccaaa gaaatgcaca ctaaaattca tcttcctccc
       61 tgcatcttac ttcttcttct gttctcacta ccatctttca atgttgttgt aggtggagat
      121 ggtgaatctg gtaacccatt tacacccaaa ggttatctga ttaggtactg gaagaaacaa
      181 atctcaaatg acttaccaaa gccatggttt cttctgaaca aggcatctcc attgaatgct
      241 gcacaatatg caacttacac taaacttgtt gctgatcaaa atgcactcac cacacagctc
      301 catacctttt gctcttcagc aaatctcatg tgtgcaccag atctgtcacc aagtcttgaa
      361 aaacacagtg gagatatcca ttttgccact tacagtgaca aaaactttac caattatgga
      421 accaatgaac ctggaattgg agttaacact ttcaagaact actctgaagg agaaaacatc
      481 cctgtaaatt ctttcaggcg atatggtaga ggttctcccc gtgacaataa atttgacaat
      541 tacgcctctg atggcaatgt tattgaccaa agtttcaatt cctatagcac aagtactgct
      601 ggaggttcag gcaaattcac aaattacgcg gcgaatgcca atgaccccaa tctgcatttc
      661 acttcctatt ccgatcaagg aacaggaggt gtacagaaat tcacaatata ctcacaagaa
      721 gccaatgctg gtgaccagta tttcaaaagt tacggcaaaa atgggaatgg tgctaatggt
      781 gaattcgtca gctatggaaa tgacacaaat gttatcggct caacatttac aaattatggt
      841 cagacagcaa atgggggaga ccaaaaattc acatcttatg gtttcaacgg caatgttcct
      901 gaaaatcatt tcaccaacta tggtgctgga ggtaatggtc catctgaaac ttttaatagt
      961 tacagagatc aatcgaatgt tggagatgac acattcacta cctatgttaa ggatgcaaat
     1021 ggcggtgaag cgaatttcac caactatggt caatcattca atgaaggtac tgatgtattc
     1081 actacttacg gcaaaggggg taatgaccca catatcaatt tcaaaactta cggagttaac
     1141 aacactttca aagattatgt caaagatact gctacatttt ccaattacca caacaaaact
     1201 tcccaagttt tagcatcgtt gatggaggtc aacggtggta aaaaggtgaa taaccggtgg
     1261 gttgagcccg gaaagttttt ccgggagaag atgttgaaga gtggtacaat catgcctatg
     1321 ccagatataa aggataagat gcctaaaagg tcctttttgc cccgggtgat tgcttccaaa
     1381 ttaccatttt ctacttcaaa aattgctgag ctgaagaaaa tcttccacgc cggtgatgag
     1441 tctcaggtgg agaagatgat cggcgatgca ttgagtgagt gtgaaagagc accgagcgcc
     1501 ggtgagacga aacgatgtgt taattcagct gaagatatga ttgatttcgc aacatcagtg
     1561 ttgggtcgaa acgtcgtcgt tcgaacgact gaggatacaa aaggatcaaa tgggaatatc
     1621 atgattggat cagtcaaagg aatcaacggt ggaaaagtta ctaaatcagt atcatgtcat
     1681 caaacgctgt acccttactt actgtattac tgtcattcgg ttcctaaagt ccgggtctac
     1741 gaagcggata ttttggaccc gaattcaaag gttaagatca atcatggtgt cgcgatttgc
     1801 cacgtggata catcttcatg gggaccgagt cacggagcgt ttgtcgcact cgggtcggga
     1861 cccgggaaaa tagaagtttg tcattggatc tttgagaatg atatgacttg ggcaattgct
     1921 gattgagaaa aaaaaaagaa atgaaataat atgcaaaatt tctaattcgg gtcgaaccgg
     1981 gtgtgttaca agaagaagaa aaaaggtacc actggtttga cttttatagt aattattatt
     2041 attatagtct taatttatat tttgagtaat tttcgtgtaa gtttctcttt gccttcatta
     2101 agtatgaatg gctatcaatt tacactattt gttatgtaat cattttattg ttgactcata
     2161 tttgagcaag gtaatgtagt tattgccaga tg
//