From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: D.melanogaster membrane protein (patched) gene, complete cds.
Date: 13 Jan 93 05:46:28 GMT
Distribution: bionet
Checksum: 14882 11

LOCUS       DROMPP2      5665 bp ds-DNA             INV       12-JAN-1993
DEFINITION  D.melanogaster membrane protein (patched) gene, complete cds.
ACCESSION   M28999
KEYWORDS    transmembrane protein.
SEGMENT     2 of 2
SOURCE      D.melanogaster (embryo), DNA and cDNA to mRNA.
  ORGANISM  Drosophila melanogaster
            Eukaryota; Animalia; Metazoa; Arthropoda; Uniramia; Insecta;
            Pterygota; Neoptera; Holometabola; Diptera; Brachycera;
            Cyclorrhapha; Schizophora; Drosophiloidea; Drosophilidae.
REFERENCE   1  (bases 1 to 5665)
  AUTHORS   Hooper,J.E. and Scott,M.P.
  TITLE     The Drosophila patched gene encodes a putative membrane protein
            required for segmental patterning
  JOURNAL   Cell 59, 751-765 (1989)
  STANDARD  full automatic
COMMENT     Draft entry and computer-readable sequence for [1] kindly provided
            by J.E.Hooper, 27-SEP-1989.
               Mak called J.E.Hooper and requested copy of cds be sent showing
            introns and exons, 9-OCT-1989.  Copy was received and corrections
            made, 18-OCT-1989.
FEATURES             Location/Qualifiers
     intron          <1..94
                     /note="MPR intron A; 9.2 kb gap in intron"
     exon            95..648
                     /number=2
     intron          649..985
                     /note="MPR intron B"
     exon            986..2912
                     /number=3
     intron          2913..3050
                     /note="MPR intron C"
     exon            3051..3258
                     /number=4
     intron          3259..3321
                     /note="MPR intron D"
     exon            3322..3593
                     /number=5
     intron          3594..3788
                     /note="MPR intron E"
     exon            3789..>4559
                     /number=6
                     /note="membrane protein"
     CDS             join(M28418:3161..3289,95..648,986..2912,3051..3258,
                     3322..3593,3789..4559)
                     /gene="patched"
                     /note="membrane protein"
                     /codon_start=1
                     /translation="MDRDSLPRVPDTHGDVVDEKLFSDLYIRTSWVDAQVALDQIDKG
                     KARGSRTAIYLRSVFQSHLETLGSSVQKHAGKVLFVAILVLSTFCVGLKSAQIHSKVH
                     QLWIQEGGRLEAELAYTQKTIGEDESATHQLLIQTTHDPNASVLHPQALLAHLEVLVK
                     ATAVKVHLYDTEWGLRDMCNMPSTPSFEGIYYIEQILRHLIPCSIITPLDCFWEGSQL
                     LGPESAVVIPGLNQRLLWTTLNPASVMQYMKQKMSEEKISFDFETVEQYMKRAAIGSG
                     YMEKPCLNPLNPNCPDTAPNKNSTQPPDVGAILSGGCYGYAAKHMHWPEELIVGGRKR
                     NRSGHLRKAQALQSVVQLMTEKEMYDQWQDNYKVHHLGWTQEKAAEVLNAWQRNFSRE
                     VEQLLRKQSRIATNYDIYVFSSAALDDILAKFSHPSALSIVIGVAVTVLYAFCTLLRW
                     RDPVRGQSSVGVAGVLLMCFSTAAGLGLSALLGIVFNAASTQVVPFLALGLGVDHIFM
                     LTAAYAESNRREQTKLILKKVGPSILFSACSTAGSFFAAAFIPVPALKVFCLQAAIVM
                     CSNLAAALLVFPAMISLDLRRRTAGRADIFCCCFPVWKEQPKVAPPVLPLNNNNGRGA
                     RHPKSCNNNRVPLPAQNPLLEQRADIPGSSHSLASFSLATFAFQHYTPFLMRSWVKFL
                     TVMGFLAALISSLYASTRLQDGLDIIDLVPKDSNEHKFLDAQTRLFGFYSMYAVTQGN
                     FEYPTQQQLLRDYHDSFVRVPHVIKNDNGGLPDFWLLLFSEWLGNLQKIFDEEYRDGR
                     LTKECWFPNASSDAILAYKLIVQTGHVDNPVDKELVLTNRLVNSDGIINQRAFYNYLS
                     AWATNDVFAYGASQGKLYPEPRQYFHQPNEYDLKIPKSLPLVYAQMPFYLHGLTDTSQ
                     IKTLIGHIRDLSVKYEGFGLPNYPSGIPFIFWEQYMTLRSSLAMILACVLLAALVLVS
                     LLLLSVWAAVLVILSVLASLAQIFGAMTLLGIKLSAIPAVILILSVGMMLCFNVLISL
                     GFMTSVGNRQRRVQLSMQMSLGPLVHGMLTSGVAVFMLSTSPFEFVIRHFCWLLLVVL
                     CVGACNSLLVFPILLSMVGPEAELVPLEHPDRISTPSPLPVRSSKRSGKSYVVQGSRS
                     SRGSCQKSHHHHHKDLNDPSLTTITEEPQSWKSSNSSIQMPNDWTYQPREQRPASYAA
                     PPPAYHKAAAQQHHQHQGPPTTPPPPFPTAYPPELQSIVVQPEVTVETTHSDSNTTKV
                     TATANIKVELAMPGRAVRSYNFTS"
BASE COUNT     1397 a   1537 c   1350 g   1381 t
ORIGIN      About 9.2kb after segment 1, Chromosome 2, map position 44D-E.
        1 aattttaatg cgtattttat ggcagtggag caaggcgggg gaatctaaaa aaaaaactaa
       61 acgctaaatt ccgtattttt gttgcatttt tcagggcaaa gcgcgtggca gccgcacggc
      121 gatctatctg cgatcagtat tccagtccca cctcgaaacc ctcggcagct ccgtgcaaaa
      181 gcacgcgggc aaggtgctat tcgtggctat cctggtgctg agcaccttct gcgtcggcct
      241 gaagagcgcc cagatccact ccaaggtgca ccagctgtgg atccaggagg gcggccggct
      301 ggaggcggaa ctggcctaca cacagaagac gatcggcgag gacgagtcgg ccacgcatca
      361 gctgctcatt cagacgaccc acgacccgaa cgcctccgtc ctgcatccgc aggcgctgct
      421 tgcccacctg gaggtcctgg tcaaggccac cgccgtcaag gtgcacctct acgacaccga
      481 atgggggctg cgcgacatgt gcaacatgcc gagcacgccc tccttcgagg gcatctacta
      541 catcgagcag atcctgcgcc acctcattcc gtgctcgatc atcacgccgc tggactgttt
      601 ctgggaggga agccagctgt tgggtccgga atcagcggtc gttataccgt aagtagttaa
      661 tatgtagtta atagccacat cttatagatt ctaaagtgaa cgtatccctt atgaccatat
      721 ccttttgcat gatctacttt aacccacagt acttctctat tcatattaag gaattaataa
      781 agtacttact ttgcgcttac ctttattaaa tacgatagct tatctttata aacttgctat
      841 caagtcgaaa gataaacgtg acaagagtat ctttgtactt atcccagttg cttaccatcg
      901 taaataatct tcttattaat aaatattcgt aaataaatat tcttaactca acaaatccat
      961 ctttattatt gttactcctc tacagaggcc tcaaccaacg actcctgtgg accaccctga
     1021 atcccgcctc tgtgatgcag tatatgaaac aaaagatgtc cgaggaaaag atcagcttcg
     1081 acttcgagac cgtggagcag tacatgaagc gtgcggccat tggcagtggc tacatggaga
     1141 agccctgcct gaacccactg aatcccaatt gcccggacac ggcaccgaac aagaacagca
     1201 cccagccgcc ggatgtggga gccatcctgt ccggaggctg ctacggttat gccgcgaagc
     1261 acatgcactg gccggaggag ctgattgtgg gcggacggaa gaggaaccgc agcggacact
     1321 tgaggaaggc ccaggccctg cagtcggtgg tgcagctgat gaccgagaag gaaatgtacg
     1381 accagtggca ggacaactac aaggtgcacc atcttggatg gacgcaggag aaggcagcgg
     1441 aggttttgaa cgcctggcag cgcaactttt cgcgggaggt ggaacagctg ctacgtaaac
     1501 agtcgagaat tgccaccaac tacgatatct acgtgttcag ctcggctgca ctggatgaca
     1561 tcctggccaa gttctcccat cccagcgcct tgtccattgt catcggcgtg gccgtcaccg
     1621 ttttgtatgc cttttgcacg ctcctccgct ggagggaccc cgtccgtggc cagagcagtg
     1681 tgggcgtggc cggagttctg ctcatgtgct tcagtaccgc cgccggattg ggattgtcag
     1741 ccctgctcgg tatcgttttc aatgccgcca gcacccaggt ggttccgttt ttggcccttg
     1801 gtctgggcgt cgatcacatc ttcatgctga ccgctgccta tgcggagagc aatcggcggg
     1861 agcagaccaa gctgattctc aagaaagtgg gaccgagcat cctgttcagt gcctgcagca
     1921 ccgcaggatc cttctttgcg gccgccttta ttccggtgcc ggctttgaag gtattctgtc
     1981 tgcaggctgc catcgtaatg tgctccaatt tggcagcggc tctattggtt tttccggcca
     2041 tgatttcgtt ggatctacgg agacgtaccg ccggcagggc ggacatcttc tgctgctgtt
     2101 ttccggtgtg gaaggaacag ccgaaggtgg cacctccggt gctgccgctg aacaacaaca
     2161 acgggcgcgg ggcccggcat ccgaagagct gcaacaacaa cagggtgccg ctgcccgccc
     2221 agaatcctct gctggaacag agggcagaca tccctgggag cagtcactca ctggcgtcct
     2281 tctccctggc aaccttcgcc tttcagcact acactccctt cctcatgcgc agctgggtga
     2341 agttcctgac cgttatgggt ttcctggcgg ccctcatatc cagcttgtat gcctccacgc
     2401 gccttcagga tggcctggac attattgatc tggtgcccaa ggacagcaac gagcacaagt
     2461 tcctggatgc tcaaactcgg ctctttggct tctacagcat gtatgcggtt acccagggca
     2521 actttgaata tcccacccag cagcagttgc tcagggacta ccatgattcc tttgtgcggg
     2581 tgccacatgt gatcaagaat gataacggtg gactgccgga cttctggctg ctgctcttca
     2641 gcgagtggct gggtaatctg caaaagatat tcgacgagga ataccgcgac ggacggctga
     2701 ccaaggagtg ctggttccca aacgccagca gcgatgccat cctggcctac aagctaatcg
     2761 tgcaaaccgg ccatgtggac aaccccgtgg acaaggaact ggtgctcacc aatcgcctgg
     2821 tcaacagcga tggcatcatc aaccaacgcg ccttctacaa ctatctgtcg gcatgggcca
     2881 ccaacgacgt cttcgcctac ggagcttctc aggtgggtct tcttattaaa ttaaattaaa
     2941 ttaaattaaa ttagatcgcc ttagttctcc tcatatgtac atacatatta taacttatcg
     3001 cactccaaag ttaaagatta ctaaatgtgt gtgtatcttt attcttacag ggcaaattgt
     3061 atccggaacc gcgccagtat tttcaccaac ccaacgagta cgatcttaag atacccaaga
     3121 gtctgccatt ggtctacgct cagatgccct tttacctcca cggactaaca gatacctcgc
     3181 agatcaagac cctgataggt catattcgcg acctgagcgt caagtacgag ggcttcggcc
     3241 tgcccaacta tccatcgggt gagtcggaaa tgagtacttc atacatgggg cccaactaac
     3301 agtcgattta tttatcgcca ggcattccct tcatcttctg ggagcagtac atgaccctgc
     3361 gctcctcact ggccatgatc ctggcctgcg tgctactcgc cgccctggtg ctggtctccc
     3421 tgctcctgct ctccgtttgg gccgccgttc tcgtgatcct cagcgttctg gcctcgctgg
     3481 cccagatctt tggggccatg actctgctgg gcatcaaact ctcggccatt ccggcagtca
     3541 tactcatcct cagcgtgggc atgatgctgt gcttcaatgt gctgatatca ctggtgagtc
     3601 ttcatttctg gctggaccat taagagcttc ggagtgagtc ttcatttctg gctggaccat
     3661 taagagcttc ggagtgagtc ttcatttctg gctggaccat taagagcttc ggattttcca
     3721 gagatatccc aagacttttc attggatcct cttcagcaca cattaattgc ttatctttcc
     3781 gattctaggg cttcatgaca tccgttggca accgacagcg ccgcgtccag ctgagcatgc
     3841 agatgtccct gggaccactt gtccacggca tgctgacctc cggagtggcc gtgttcatgc
     3901 tctccacgtc gccctttgag tttgtgatcc ggcacttctg ctggcttctg ctggtggtct
     3961 tatgcgttgg cgcctgcaac agccttttgg tgttccccat cctactgagc atggtgggac
     4021 cggaggcgga gctggtgccg ctggagcatc cagaccgcat atccacgccc tctccgctgc
     4081 ccgtgcgcag cagcaagaga tcgggcaaat cctatgtggt gcagggatcg cgatcctcgc
     4141 gaggcagctg ccagaagtcg catcaccacc accacaaaga ccttaatgat ccatcgctga
     4201 cgacgatcac cgaggagccg cagtcgtgga agtccagcaa ctcgtccatc cagatgccca
     4261 atgattggac ctaccagccg cgggaacagc gacccgcctc ctacgcggcc ccgccccccg
     4321 cctatcacaa ggccgccgcc cagcagcacc accagcatca gggcccgccc acaacgcccc
     4381 cgcctccctt cccgacggcc tatccgccgg agctgcagag catcgtggtg cagccggagg
     4441 tgacggtgga gacgacgcac tcggacagca acaccaccaa ggtgacggcc acggccaaca
     4501 tcaaggtgga gctggccatg cccggcaggg cggtgcgcag ctataacttt acgagttagc
     4561 actagcacta gttcctgtag ctattaggac gtatctttag actctagcct aagccgtaac
     4621 cctatttgta tctgtaaaat cgatttgtcc agcgggtctg ctgaggattt cgttctcatg
     4681 gattctcatg gattctcatg gatgcttaaa tggcatggta attggcaaaa tatcaatttt
     4741 tgtgtctcaa aaagatgcat tagcttatgg tttcaagata catttttaaa gagtccgcca
     4801 gatatttata taaaaaaaat ccaaaatcga cgtatccatg aaaattgaaa agctaagcag
     4861 acccgtatgt atgtatatgt gtatgcatgt tagttaattt cccgaagtcc ggtatttata
     4921 gcagctgcct tccgcgcccc ccttcccttg aaatgaacac ccttccagcc acgccccacc
     4981 gcccctctgc gtagcagctt tgtatgtatg tagtatgcta gcacctaagg aatacttaaa
     5041 cttagagata tttattgtaa cacacgcaaa acacacacaa tgtacttaca tataattcaa
     5101 tgcgagattc acccacacaa aaaggaaaca caacaaacta gtaattgtag ctcgtaattt
     5161 agtttaaata tgttacataa aacacaagga cttgaaccaa aatagtatcg cttaaacgga
     5221 aacgagagaa acgagaaaaa ataactatta cttaatcaac tacaagagag atatccctcc
     5281 tcccctaacc gtacttacaa ccaaaataaa acaagagtat aagcataaaa atggaaaacg
     5341 aagcgaggaa cgattgtaaa cgcggtcatt tatcctgtac atttgttgcc cgaagactga
     5401 ctgtcttttt tttaataaaa atatatatta tacagttttt taaaagcgaa attcatgact
     5461 tttttttaac agtgagcaga gaacaaaaga aacggaagtt ttcgctgtat caataaaaag
     5521 attccatttt tttaataaat tgtaaaaatc ctaaaaaaaa gaagactaca aaagtttaaa
     5581 tttttatacg ttattgataa acttttatac acgaaaatac ttgtacttag ctatgatcaa
     5641 ctccttggct taagtctcgg gtaag
//