From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: B. bronchiseptica prn gene for P.68 pertactin
Date: 3 Jan 93 22:03:49 GMT
Distribution: bionet
Checksum: 37503 7

LOCUS       BPEPRNG      3000 bp ds-DNA             BCT       21-DEC-1992
DEFINITION  B. bronchiseptica prn gene for P.68 pertactin
ACCESSION   X54815
KEYWORDS    P.68 pertactin; outer membrane protein; prn gene.
SOURCE      Bordetella bronchiseptica DNA.
  ORGANISM  Bordetella bronchiseptica
            Prokaryotae; Gracilicutes; Scotobacteria; Aerobic rods and cocci;
            Alcaligenaceae.
REFERENCE   1  (bases 1 to 3000)
  AUTHORS   Jing-Li,L., Fairweather,N.F., Novotny,P., Dougan,G. and
            Charles,I.G.
  TITLE     Cloning, nucleotide sequence and expression of the protective outer
            membrane protein P.68 pertactin from Bordetella bronchiseptica
  JOURNAL   J. Gen. Microbiol. 138, 1697-1705 (1992)
  STANDARD  full automatic
COMMENT     *source: clone_library=cosmid pMC79; *source: isolate=LBF(CN7635);

               From EMBL    entry BBPRNG;  dated 03-DEC-1992.
FEATURES             Location/Qualifiers
     sig_peptide     145..246
     mat_peptide     247..2877
                     /product="P.68 Peractin"
                     /gene="prn"
     CDS             145..2880
                     /gene="prn"
                     /product="P.68 Peractin"
                     /codon_start=1
                     /translation="MNMSLSRIVLAAPLRRTTLAMALGALGAAPAAYADWNNQSIIKA
                     GERQHGIHIKQSDGAGVRTATGTTIKVSGRQAQGVLLENPAAELRFQNGSVTSSGQLF
                     DEGVRRFLGTVTVKAGKLVADHATLANVSDTRDDDGIALYVAGEQAQASIADSTLQGA
                     GGVRVERGANVTVQRSTIVDGGLHIGTLQPLQPEDLPPSRVVLGDTSVTAVPASGAPA
                     AVSVFGANELTVDGGHITGGRAAGVAAMDGAIVHLQRATIRRGDAPAGGAVPGGAVPG
                     GFGPLLDGWYGVDVSDSTVDLAQSIVEAPQLGAAIRAGRGARVTVSGGSLSAPHGNVI
                     ETGGGARRFPPPASPLSITLQAGARAQGRALLYRVLPEPVKLTLAGGAQGQGDIVATE
                     LPPIPGASSGPLDVALASQARWTGATRAVDSLSIDNATWVMTDNSNVGALRLASDGSV
                     DFQQPAEAGRFKCLMVDTLAGSGLFRMNVFADLGLSDKLVVMRDASGQHRLLVRNSGS
                     EPASGNTMLLVQTPRGSAATFTLANKDGKVDIGTYRYRLAANGNGQWSLVGAKAPPAP
                     KPAPQPGPQPGPQPPQPPQPPQPPQRQPEAPAPQPPAGRELSAAANAAVNTGGVGLAS
                     TLWYAESNALSKRLGELRLNPDAGGAWGRGFAQRQQLDNRAGRRFDQKVAGFELGADH
                     AVAVAGGRWHLGGLAGYTRGDRGFTGDGGGHTDSVHVGGYATYIANSGFYLDATLRAS
                     RLENDFKVAGSDGYAVKGKYRTHGVGASLEAGRRFAHADGWFLEPQAELAVFRVGGGS
                     YRAANGLRVRDEGGSSVLGRLGLEVGKRIELAGGRQVQPYIKASVLQEFDGAGTVRTN
                     GIAHRTELRGTRAELGLGMAAALGRGHSLYASYEYSKGPKLAMPWTFHAGYRYSW"
BASE COUNT      450 a   1025 c   1102 g    423 t
ORIGIN      
        1 atcgatgatg cgtcgctgta acacggcaaa taccgtgcat tgcagcggtt ctggatggcg
       61 ttcttcgtac gtttgctgcg cccattcttc cctgttccat cgcggtgcgg ccatggcggg
      121 cgtctgctct tcacccggca tccaatgaac atgtctctgt cacgcattgt cttggcggcg
      181 cccctgcgcc gcaccacact ggccatggcg ctgggcgcgc tgggcgccgc gcccgccgcg
      241 tacgccgact ggaacaacca gtccatcatc aaggccggcg agcgccagca cggcatccac
      301 atcaagcaaa gcgatggcgc cggcgtacgg accgccaccg gaacgaccat caaggtaagc
      361 ggtcgtcagg cccagggcgt cctgctggaa aatcccgcgg ccgagctgcg gttccagaac
      421 ggcagcgtca cgtcttcggg acagctgttc gacgaaggcg tccggcgctt tctgggcacc
      481 gtcaccgtca aggccggcaa gctggtcgcc gatcacgcca cgctggccaa cgtcagcgac
      541 acccgggacg acgacggcat cgcgctctat gtggccggcg agcaggccca ggccagcatc
      601 gccgacagca ccctgcaggg cgcgggcggc gtgcgggtcg agcgcggcgc caatgtcacg
      661 gtccaacgca gcaccatcgt tgacgggggc ttgcatatcg gcaccctgca gccgctgcag
      721 ccggaagacc ttccgcccag ccgggtggtg ctgggcgaca ccagcgtgac cgccgtgccc
      781 gccagcggcg cgcccgcggc ggtgtctgta ttcggggcca atgagcttac ggttgatggc
      841 gggcacatca ccggggggcg ggcagcgggg gtggcggcca tggacggggc gatcgtgcat
      901 ctgcagcgcg cgacgatacg gcggggggac gcgcctgccg gcggtgcggt tccaggcggt
      961 gctgttcccg gcggcttcgg ccccctcctt gacggctggt atggcgtgga tgtatcggat
     1021 tccaccgtgg acctcgctca gtcgatcgtc gaggcgccgc agctgggcgc cgcgatccgg
     1081 gcgggccgcg gcgccagggt gacggtgtcg ggcggcagct tgtccgcacc gcacggcaat
     1141 gtcatcgaga ccggcggcgg cgcgcgtcgc ttcccgcctc cggcctcgcc cctgtcgatc
     1201 accttgcagg cgggcgcacg ggcgcagggg agggcgctgc tgtaccgggt cctgccggag
     1261 cccgtgaagc tgacgctggc gggcggcgcc caggggcagg gcgacatcgt cgcgacggag
     1321 ctgcctccca ttccaggcgc gtcgagcggg ccgctcgacg tggcgctggc cagccaggcc
     1381 cgatggacgg gcgctacccg cgcggtcgac tcgctgtcca tcgacaacgc cacctgggtc
     1441 atgacggaca actcgaacgt cggcgcgctg cggctggcca gcgacggcag cgtcgatttc
     1501 cagcagccgg ccgaagctgg gcggttcaag tgcctgatgg tcgatacgct ggcgggttcg
     1561 gggctgttcc gcatgaatgt cttcgcggac ctggggctga gcgacaagct ggtcgtcatg
     1621 cgggacgcca gcggccagca caggctgttg gtccgcaaca gcggcagcga gccggccagc
     1681 ggcaacacca tgctgctggt gcagacgcca cgaggcagcg cggcgacctt tacccttgcc
     1741 aacaaggacg gcaaggtcga tatcggtacc taccgctatc gattggccgc caacggcaat
     1801 gggcagtgga gcctggtggg cgcgaaggcg ccgccggcgc ccaagcccgc gccgcagccc
     1861 ggtccccagc ccggtcccca gccgccgcag ccgccgcagc cgccgcagcc gccacagagg
     1921 cagccggaag cgccggcgcc gcaaccgccg gcgggcaggg agttgtccgc cgccgccaac
     1981 gcggcggtca acacgggtgg ggtgggcctg gccagcacgc tctggtacgc cgaaagcaat
     2041 gcgttgtcca agcgcctggg cgagttgcgc ctgaatccgg acgccggcgg cgcttggggc
     2101 cgcggcttcg cgcaacgcca gcaactggac aaccgcgccg ggcggcgctt cgaccagaag
     2161 gtggccggct tcgagctggg cgccgaccac gcggtggcgg tggccggcgg gcgctggcac
     2221 ctgggcgggc tggccggcta tacgcgcggc gaccgcggct ttaccggcga cggcggcggc
     2281 cacaccgaca gcgtgcatgt cgggggctat gccacctata tcgccaacag cggtttctac
     2341 ctggacgcga cgctgcgcgc cagccgcctc gaaaatgact tcaaggtggc gggcagcgat
     2401 gggtacgcgg tcaagggcaa gtaccgcacc catggggtag gcgcctcgct cgaggcgggc
     2461 cggcgcttcg cccatgccga cggctggttc ctcgagccgc aggccgagct ggcggtgttc
     2521 cgggtcggcg gcggttcgta ccgcgcggcc aatggcctgc gggtgcgcga cgaaggcggc
     2581 agctcggtgc tgggtcgcct gggcctggag gtcggcaagc gcatcgaact ggcaggcggc
     2641 aggcaggtgc agccatacat caaggccagc gtgctgcagg agttcgacgg cgcgggtacg
     2701 gtacgcacca acggcatcgc gcaccgcacc gaactgcgcg gcacgcgcgc cgaactgggc
     2761 ctgggcatgg ccgccgcgct gggccgcggc cacagcctgt atgcctcgta cgagtactcc
     2821 aagggcccga agctggccat gccgtggacc ttccacgcgg gctaccggta cagctggtaa
     2881 agcgagaagg gtccatcccc ccgcggggga gattttcctg gaggttggcc ggtgccagtc
     2941 tccaggctca ggcggccagg gcgtgcgggc cgggcaggcc gtgctggtgc tggccgaacc
//