From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: E. carotovora pectate lyase III gene.
Date: 9 Apr 92 10:02:52 GMT
Distribution: bionet
Checksum: 27428     4

LOCUS       ERWPEL3      1415 bp ds-DNA             BCT       09-APR-1992
DEFINITION  E. carotovora pectate lyase III gene.
ACCESSION   D10064 D01243
KEYWORDS    pectate lyase III.
SOURCE      Erwinia carotovora Er genomic DNA.
  ORGANISM  Erwinia carotovora
            Prokaryota; Bacteria; Gram-negative facultatively anaerobic rods;
            Enterobacteriaceae.
REFERENCE   1  (bases 1 to 1415)
  AUTHORS   Yoshida,A., Izuta,M., Ito,K., Kamio,Y. and Izaki,K.
  TITLE     Cloning and characterization of the pectate lyase III gene of
            Erwinia carotovora Er
  JOURNAL   Agric. Biol. Chem. 55, 933-940 (1991)
  STANDARD  full staff_review
COMMENT     Data kindly submitted in computer readable form by:
            Aruto Yoshida
            Department of Agricultural Chemistry
            Tohoku University
            1-1 Tsutsumi-dori, Amamiya-cho
            Aoba-ku, Sendai 981
            Japan
            Phone:  022-272-4321 x277
            Fax:    022-272-1870
FEATURES             Location/Qualifiers
     RBS             204..208
     CDS             218..1342
                     /EC_number="4.2.2.2"
                     /product="pectate lyase III precursor"
                     /gene="pel III"
                     /codon_start=1
                     /translation="MKYLLPSAAAGLLLLAAQPTMAANTGGYATTDGGDVAGAVKKTA
                     RSMQDIIDIIEAAKLDSNGKKVKGGAYPLVITYNGNEDALIKAAEANICGQWSKDARG
                     VEIKEFTKGITIIGTNGSSANFGIWLTKSSDIVIRNMRFGYMPGGAQDGDAIRIDNTP
                     NVWIDHNEIFAKNFECAGTKDGDTTFESAIDIKKASTNVTISYNYIHGIKKVGLSGFS
                     SSDTGRDLTYHHNIYDDVNARLPLQRGGQVHAYNNLYTGITSSGLNVRQKGIALIERN
                     WFENAKNPVTSRYDGSNFGTWELRNNNVMSPADFAKYNITWDKDSKPYVNAEDWKSTG
                     TFASVPYSYSPVSAQCVKDKLANYAGVNKNLAVLTAANCN"
     sig_peptide     218..283
                     /codon_start=1
     mat_peptide     284..1339
                     /product="pectate lyase III mature peptide"
                     /codon_start=1
BASE COUNT      412 a    334 c    328 g    341 t
ORIGIN
        1 tttaaaatag atacttatta agcattgatg tttccatcat tattcatgag atatatttaa
       61 tacctaaata ttgaattatt tttcttttat ggtaagactt atcttggttt tcaataaacc
      121 ttgatttcat ttttattgaa acaccaaaat aatataatct gggttatgtg gaacattaat
      181 acccaaacaa aaattcaatt ccaaggagag taccttaatg aaatacctac tgccttctgc
      241 agccgctggg ctgttattgc ttgcggccca accaacgatg gcggcaaata cggggggtta
      301 tgccaccact gacggtggtg acgttgccgg tgccgtgaaa aaaacagcgc gctccatgca
      361 agatattatt gatatcatcg aagccgcgaa gctggattcc aatggcaaga aagtgaaagg
      421 tggcgcttac ccgcttgtca tcacctataa cggtaatgaa gacgcgctga tcaaagccgc
      481 tgaggccaac atctgcggcc agtggagtaa ggatgcccga ggtgtagaaa tcaaagaatt
      541 caccaaaggg attaccatca tcggaaccaa tggatcgtcc gccaacttcg ggatctggct
      601 gacaaaatca tccgatatcg tcatacgtaa catgcgtttt ggttacatgc cgggcggcgc
      661 gcaggatggt gatgccattc gtatcgataa cacgccgaac gtctggattg accacaacga
      721 aatcttcgcg aaaaactttg aatgcgcagg tacaaaagac ggtgacacga catttgaatc
      781 cgcgattgat atcaaaaaag cgtcaaccaa cgtgaccatt tcgtacaact acattcacgg
      841 catcaaaaag gtcggcctga gcggcttcag cagcagcgat acgggccgtg atctgactta
      901 tcatcacaat atttacgacg acgttaacgc tcgcctcccg ctgcaacgtg gcggccaggt
      961 tcacgcgtac aacaacctgt atactggcat caccagttct ggcctgaacg tgcgtcagaa
     1021 agggattgcg ctgatcgaac gtaactggtt cgagaatgcg aaaaacccgg tgacctcacg
     1081 ttacgacggt tccaacttcg gtacctggga gctgcgtaat aacaacgtca tgagcccggc
     1141 tgatttcgct aaatacaaca tcacctggga taaagattcc aagccctacg tgaatgcaga
     1201 agactggaaa agcactggca cattcgcttc tgttccttac agctactctc cggtttcggc
     1261 acagtgcgtg aaggacaaac tggcgaacta tgctggcgtg aacaaaaatc ttgcggtgct
     1321 gacggcagca aattgcaact aattgcgatg tgtaaagcat gaaaggtaaa taagcggact
     1381 tcaggctccg accctcattg tcctgtgcta accgg
//