From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: E. coli ads(folD) gene encoding 5,10-methylene-tetrahydrofolate
Date: 21 Jul 92 10:04:13 GMT
Distribution: bionet
Checksum: 17131     5

LOCUS       ECOADS       1769 bp ds-DNA             BCT       21-JUL-1992
DEFINITION  E. coli ads(folD) gene encoding 5,10-methylene-tetrahydrofolate
            dehydrogenase/5,10-methenyl-tetrahydrofolate cyclohydrolase.
ACCESSION   D10588 D90533
KEYWORDS    5,10-methenyl-tetrahydrofolate cyclohydrolase;
            5,10-methylene-tetrahydrofolate dehydrogenase; adenine sensitivity;
            tetrahydrofolate metabloism.
SOURCE      Escherichia coli (strain K12) DNA.
  ORGANISM  Escherichia coli
            Prokaryotae; Gram-negative facultatively anaerobic rods;
            Enterobacteriaceae.
REFERENCE   1  (bases 1 to 1769)
  AUTHORS   Yonetani,Y., Sanpei,G. and Mizobuchi,K.
  TITLE     Cloning and nucleotide sequence ananlysis of a novel
            adeninesensitive mutation of Escherichia coli strain K12 W3110
  JOURNAL   Unpublished (1992)
  STANDARD  full staff_review
COMMENT     Data kindly submitted in computer readable form by:
            Kiyoshi Mizobuchi
            Department of Biophysics and Biochemistry
            Faculty of Science,
            University of Tokyo
            Hongo
            Tokyo 113
            Japan
            Phone:  03-3812-2111 x4417
            Fax:    03-5684-2394
FEATURES             Location/Qualifiers
     misc_feature    110
                     /note="transcription start site"
     CDS             150..1016
                     /product="5,10-methylene-tetrahydrofolate dehydrogenase/5,
                     10-methenyl-tetrahydrofolate cyclohydrolase"
                     /gene="ads(folD)"
                     /codon_start=1
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDTLNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQDE"
BASE COUNT      369 a    474 c    484 g    442 t
ORIGIN      12 min on K12 map.
        1 aagctttcag tctcagcgac ggtgcgcctc accttcgcaa gaggtcgctt cacgcgataa
       61 atctgaaacg aaacctgaca gcgcgccccg cttctgacaa aataggcgca tccccttcga
      121 tctacgtaac agatggaatc ctctctctga tggcagcaaa gattattgac ggtaaaacga
      181 ttgcgcagca ggtgcgctct gaagttgctc aaaaagttca ggcgcgtatt gcagccggac
      241 tgcgggcacc aggactggcc gttgtgctgg tgggtagtaa ccctgcatcg caaatttatg
      301 tcgcaagcaa acgcaaggct tgtgaagaag tcgggttcgt ctcccgctct tatgacctcc
      361 cggaaaccac cagcgaagcg gagctgctgg agcttatcga tacgctgaat gccgacaaca
      421 ccatcgatgg cattctggtt caactgccgt taccggcggg tattgataac gtcaaagtgc
      481 tggaacgtat tcatccggac aaagacgtgg acggtttcca tccttacaac gtcggtcgtc
      541 tgtgccagcg cgcgccgcgt ctgcgtccct gcaccccgcg cggtatcgtc acgctgcttg
      601 agcgttacaa cattgatacc ttcggcctca acgccgtggt gattggcgca tcgaatatcg
      661 ttggccgccc gatgagcatg gaactgctgc tggcaggttg caccactaca gtgactcacc
      721 gcttcactaa aaatctgcgt catcacgtag aaaatgccga tctattgatc gttgccgttg
      781 gcaagccagg ctttattccc ggtgactgga tcaaagaagg cgcaattgtg attgatgtcg
      841 gcatcaaccg tctggaaaat ggcaaagttg tgggcgacgt cgtgtttgaa gacgcggcta
      901 aacgcgcctc atacattacg cctgttcccg gcggcgttgg cccgatgacg gttgccacgc
      961 tgattgaaaa cacgctacag gcgtgcgttg aatatcatga tccacaggat gagtaacatg
     1021 gcgacatttt ctttaggtaa acatccgcac gttgagctgt gcgacttgct gaaactggaa
     1081 ggctggagcg aaacggcgcg caggcgaaaa tcgcgattgc cgaaggccag gtgaaagtcg
     1141 acggtgcggt tgaaacgcgc aaacgctgca aaatcgtcgc cggtcagaca gtgagttttg
     1201 caggtcacag cgtacaggtt gttgcctgat ccctttcccg gcctgtatca acaggccggt
     1261 tctcttttca gacttttgcc ctctacgccc cgttcatcca tgctgatagc atcgacattt
     1321 tgccgaagcg accatcatgc caaccgttat tacgcacgcc gccgttcccc tatgcattgg
     1381 tttagggttg ggttcgaaag tcatccctcc gcgtctgtta tttgccggaa tcatcctggc
     1441 gatgctgccc gacgccgacg tattgtcgtt taaatttggc gttgcttacg gcaatgtttt
     1501 tggtcatcgc gggtttaccc attcgctggt gtttgcgttt gttgtcccgc tgttatgtgt
     1561 gtttattgga cgccgatggt tcagggctgg gctgattcgc tgctggctgt ttttaaccgt
     1621 ctcgttgcta tcgcacagct tgctggattc ggtaaccact ggcggtaaag gcgttggctg
     1681 gctgtggccg tggtcagatg aacgcttttt cgctccctgg caggtgataa aagtcgcgcc
     1741 gtttgcattg tctcgttaca ccacgccgc
//