From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: pBR322 cloning vector.
Date: 8 Jan 93 05:51:48 GMT
Distribution: bionet
Checksum: 23256 12

LOCUS       SYNPBR322V   4363 bp ds-DNA   Circular  SYN       07-JAN-1993
DEFINITION  pBR322 cloning vector.
ACCESSION   L08654
KEYWORDS    .
SOURCE      Artificial gene DNA; Cloning vector DNA; Cloning vector DNA;
            Cloning vector DNA; and Transposon Tn3 DNA.
  ORGANISM  Artificial gene
            Artificial sequences; Genes.
REFERENCE   1  (sites)
  AUTHORS   Sutcliffe,J.G.
  TITLE     Complete nucleotide sequence of the Escherichia coli plasmid pBR322
  JOURNAL   Cold Spring Harb. Symp. Quant. Biol. 43, 77-90 (1979)
  STANDARD  full automatic
REFERENCE   2  (sites)
  AUTHORS   Peden,K.W.
  TITLE     Revised sequence of the tetracycline-resistance gene of pBR322
  JOURNAL   Gene 22, 277-280 (1983)
  STANDARD  full automatic
REFERENCE   3  (sites)
  AUTHORS   Livneh,Z.
  TITLE     Directed mutagenesis method for analysis of mutagen specificity:
            Application to ultraviolet-induced mutagenesis
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 80, 237-241 (1983)
  STANDARD  full automatic
REFERENCE   4  (sites)
  AUTHORS   Lathe,R., Kieny,M.P., Skory,S. and Lecocoq,J.P.
  TITLE     Laboratory methods linker tailing: Unphosphorylated linker
            oligonucleotides for joining DNA termini
  JOURNAL   DNA 3, 173-182 (1984)
  STANDARD  full automatic
REFERENCE   5  (sites)
  AUTHORS   Huesterspreute,M. and Davison,J.
  TITLE     Restriction site bank vectors. II. DNA sequence analysis of plasmid
            pJRD158
  JOURNAL   DNA 3, 259-268 (1984)
  STANDARD  full automatic
REFERENCE   6  (bases 1 to 4363)
  AUTHORS   Gilbert,W.
  TITLE     Obtained from VecBase 3.0
  JOURNAL   Unpublished (1991)
  STANDARD  full automatic
COMMENT     COMMENT
              pos 3148-3185 corresponds to one of the 38 bp inverted repeats
              on Tn3 (pos 1-38 and (c)4957-4920) COMMENT
              from EMBL(9.0) PBR322
              FH   Key        From     To       Description
              FT   CDS          86   1273       tetracycline resistance gene
              FT   RBS        1906   1910       Shine-Dalgarno sequence
              FT   CDS        1918   2105       ROP-protein
              FT   ORGRPL     2537   2537       Origin of replication
              FT   RBS        4165   4161 (C)   Shine-Dalgarno sequence
              FT   CDS        4155   3298 (C)   Beta-lactamase gene (E-286)
              FT                                ampicillin resistance
              FT   REVISION   2731   2732       new: TA old: AT KEYWORDS
            CROSSREFERENCE
              #identical
                GenBank(50):pBR322, EMBL(11):pBR322
              #parent
                GenBank(50):pSC101C, GenBank(50):Trn3
              #offspring
                VecBase(3):pBR325, VecBase(3):pBR327, VecBase(3):pBR328,
                VecBase(3):pAT153, VecBase(3):pUC7, VecBase(3):pJRD158,
                VecBase(3):PiVX, VecBase(3):PiAN7, VecBase(3):pSP64,
                VecBase(3):pSP65, VecBase(3):pGEM1, VecBase(3):pGEM2,
                VecBase(3):pGEM3, VecBase(3):pGEM4, VecBase(3):pKK223,
                VecBase(3):pLBU3, VecBase(3):pTrS3, VecBase(3):pRSVNeo,
                VecBase(3):pSV2Cat, VecBase(3):M13mp9, VecBase(3):pHC79,
                VecBase(3):pV34, VecBase(3):pKTH601, VecBase(3):pKTH604,
                VecBase(3):pKTH605, VecBase(3):pKTH606, VecBase(3):YEp24,
                VecBase(3):YIp5, VecBase(3):YRp17, VecBase(3):pSP18,
            VecBase(3):pSP19,
                VecBase(3):pSP6T3, VecBase(3):pSP6T719, VecBase(3):pT712,
                VecBase(3):pT713, VecBase(3):pT7T318, VecBase(3):pT7T319,
                VecBase(3):pT7T3A18, VecBase(3):pT7T3A19, VecBase(3):pEX1,
                VecBase(3):pEX2, VecBase(3):pEX3, VecBase(3):pCKSP6,
                VecBase(3):pACYC177, VecBase(3):pKO1, VecBase(3):pKO2,
            VecBase(3):pKM1,
                VecBase(3):pKM2, VecBase(3):pMBL1, VecBase(3):pMBL604,
                VecBase(3):pMC1511, VecBase(3):pMC1871, VecBase(3):pAA37X,
                VecBase(3):pUR278, VecBase(3):pUR288, VecBase(3):pUR289,
                VecBase(3):pUR290, VecBase(3):pUR291, VecBase(3):pUR292,
                VecBase(3):pUR222

               PARENT
              Features of pBR322 (4363 bp)
                residue     source
                 1-1643     1-1643     pSC101
              1636-1762  1860-1986     pSC101
              1763-3147    xx-  xx     pMB1 (origin of replication)
              3148-4363  4957-3742 (c) Tn3

               VecBase accession number VB0001.
FEATURES             Location/Qualifiers
     CDS             86..1268
                     /product="tetracycline resistance protein"
                     /codon_start=1
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASH
                     YGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAG
                     RIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLH
                     APFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTV
                     FFIMQLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGE
                     KQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQG
                     QLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSR
                     AT"
     CDS             complement(3298..4086)
                     /product="beta-lactamase"
                     /codon_start=1
                     /translation="HPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMM
                     STFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAI
                     TMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPAA
                     MATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSR
                     GIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW"
BASE COUNT      984 a   1210 c   1134 g   1035 t
ORIGIN      
        1 ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa
       61 ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg
      121 caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt
      181 gcgggatatc gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata
      241 tgcgttgatg caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg
      301 ccgcccagtc ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac
      361 cacacccgtc ctgtggatcc tctacgccgg acgcatcgtg gccggcatca ccggcgccac
      421 aggtgcggtt gctggcgcct atatcgccga catcaccgat ggggaagatc gggctcgcca
      481 cttcgggctc atgagcgctt gtttcggcgt gggtatggtg gcaggccccg tggccggggg
      541 actgttgggc gccatctcct tgcatgcacc attccttgcg gcggcggtgc tcaacggcct
      601 caacctacta ctgggctgct tcctaatgca ggagtcgcat aagggagagc gtcgaccgat
      661 gcccttgaga gccttcaacc cagtcagctc cttccggtgg gcgcggggca tgactatcgt
      721 cgccgcactt atgactgtct tctttatcat gcaactcgta ggacaggtgc cggcagcgct
      781 ctgggtcatt ttcggcgagg accgctttcg ctggagcgcg acgatgatcg gcctgtcgct
      841 tgcggtattc ggaatcttgc acgccctcgc tcaagccttc gtcactggtc ccgccaccaa
      901 acgtttcggc gagaagcagg ccattatcgc cggcatggcg gccgacgcgc tgggctacgt
      961 cttgctggcg ttcgcgacgc gaggctggat ggccttcccc attatgattc ttctcgcttc
     1021 cggcggcatc gggatgcccg cgttgcaggc catgctgtcc aggcaggtag atgacgacca
     1081 tcagggacag cttcaaggat cgctcgcggc tcttaccagc ctaacttcga tcactggacc
     1141 gctgatcgtc acggcgattt atgccgcctc ggcgagcaca tggaacgggt tggcatggat
     1201 tgtaggcgcc gccctatacc ttgtctgcct ccccgcgttg cgtcgcggtg catggagccg
     1261 ggccacctcg acctgaatgg aagccggcgg cacctcgcta acggattcac cactccaaga
     1321 attggagcca atcaattctt gcggagaact gtgaatgcgc aaaccaaccc ttggcagaac
     1381 atatccatcg cgtccgccat ctccagcagc cgcacgcggc gcatctcggg cagcgttggg
     1441 tcctggccac gggtgcgcat gatcgtgctc ctgtcgttga ggacccggct aggctggcgg
     1501 ggttgcctta ctggttagca gaatgaatca ccgatacgcg agcgaacgtg aagcgactgc
     1561 tgctgcaaaa cgtctgcgac ctgagcaaca acatgaatgg tcttcggttt ccgtgtttcg
     1621 taaagtctgg aaacgcggaa gtcagcgccc tgcaccatta tgttccggat ctgcatcgca
     1681 ggatgctgct ggctaccctg tggaacacct acatctgtat taacgaagcg ctggcattga
     1741 ccctgagtga tttttctctg gtcccgccgc atccataccg ccagttgttt accctcacaa
     1801 cgttccagta accgggcatg ttcatcatca gtaacccgta tcgtgagcat cctctctcgt
     1861 ttcatcggta tcattacccc catgaacaga aattccccct tacacggagg catcaagtga
     1921 ccaaacagga aaaaaccgcc cttaacatgg cccgctttat cagaagccag acattaacgc
     1981 ttctggagaa actcaacgag ctggacgcgg atgaacaggc agacatctgt gaatcgcttc
     2041 acgaccacgc tgatgagctt taccgcagct gcctcgcgcg tttcggtgat gacggtgaaa
     2101 acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga
     2161 gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga
     2221 cccagtcacg tagcgatagc ggagtgtata ctggcttaac tatgcggcat cagagcagat
     2281 tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata
     2341 ccgcatcagg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     2401 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     2461 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     2521 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     2581 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     2641 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     2701 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     2761 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     2821 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     2881 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     2941 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct
     3001 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     3061 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     3121 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     3181 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     3241 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     3301 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     3361 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     3421 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     3481 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     3541 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     3601 tgccattgct gcaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     3661 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     3721 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     3781 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     3841 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     3901 cccggcgtca acacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     3961 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     4021 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     4081 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     4141 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     4201 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     4261 cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac
     4321 ctataaaaat aggcgtatca cgaggccctt tcgtcttcaa gaa
//