From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Bradyrhizobium japonicum aminotransferase (rtxA) gene, complete cds
Date: 29 Dec 92 20:58:19 GMT
Distribution: bionet
Checksum: 17448 7

LOCUS       RHBRTXAB     2947 bp ds-DNA             BCT       11-DEC-1992
DEFINITION  Bradyrhizobium japonicum aminotransferase (rtxA) gene, complete cds
            and dihydrorhizobitoxine (rtxAB) gene, complete cds.
ACCESSION   L01470
KEYWORDS    aminotransferase; dihydrorhizobitoxine synthase.
SOURCE      Bradyrhizobium japonicum DNA.
  ORGANISM  Bradyrhizobium japonicum
            Prokaryotae; Gracilicutes; Scotobacteria; Rhizobiaceae.
REFERENCE   1  (bases 1 to 2947)
  AUTHORS   Ruan,X. and Peters,N.K.
  TITLE     Cloning and characterization of Bradyrhizobium japonicum
            rhizobitoxine genes: Expression requires a -1 translational
            frameshift
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
REFERENCE   2  (bases 1 to 2947)
  AUTHORS   Ruan,X. and Peters,N.K.
  TITLE     Isolation and characterization of rhizobitoxine mutants of
            Bradyrhizobium japonium
  JOURNAL   J. Bacteriol. 174, 3467-3473 (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     CDS             446..2865
                     /function="required for rhizobitoxine biosynthesis"
                     /product="dihydrorhizobitpxine synthase"
                     /gene="rtxAB"
                     /evidence="EXPERIMENTAL"
                     /label=RtxAB
                     /codon_start=1
                     /note="-1 translational frameshift between sequences
                     1661..1922 required for RtxAB product; ORF interupted by
                     Tn5-17E"
     misc_feature    1161..1922
                     /label=Frameshift
                     /note="translational frameshift occurs within this
                     sequence to produce RtxAB gene product"
     CDS             446..1924
                     /function="required for serinol and rhizobitoxine
                     biosynthesis"
                     /gene="rtxA"
                     /label=RtxA
                     /note="ORF interupted by Tn5-18E; putative"
                     /product="aminotransferase"
                     /codon_start=1
                     /translation="MLLDLASRDGEFKEVTARMRRLMLNLLGGAKDYSVVPIQGGGSF
                     AMEAARSSFVSRNDKPLVCINGIYGERIFKILRLWGVEALKLVKRATEPLDPQEVAEQ
                     LSRNPGVTHLCLVHCETTTGIVNPLDAIIEEARRRGVKTIVDGMSSFGAIDIDLSRGG
                     PDVLVTSSNKCIEGPPGLAFVIASRELLEKAVQEPRSFVLDVTHQWLSLERTGEWRST
                     PPTHIVQATTKALEILEGEGIDARRRRYEKVRDDLVQELEGVVSPLLSAELQSPVCVA
                     FSAPSGIADQAGFDGLYRHLAAHNLYIYSKLHLATRSFRVGCIGEIQPSWIEQLGCAF
                     RTYFRSGPASSMGTPSTRQACERGVEMSSSFAKDSQLPFSAETAVLHAGYRRDPVTKA
                     VAVPIYQNTAYELDGDLNHIADVYTSRLMDSPIRGSSTRRPARWKKGMPRSTWEATRS
                     PSHQVKRQPSLPSSTCQAARWGTISSPHRISMATRGICSITR"
BASE COUNT      618 a    839 c    873 g    617 t
ORIGIN      
        1 gaattctcca acgagtgaca gtatgcgaat gtcccaagtc tcaggctcac gcaggatcag
       61 gctcatcagc aacggaacgc atcatgtccg tcaggcgcgg atcgatatcc gcacgcgatg
      121 gacccgatgt gcacgttcgt ccgaacctca acgttcgcct gaatgcacgg tttggcgtaa
      181 accgatcgac gcctagctag caatgcgatg gcgctcaaat ccttgcatgc atgatgtggt
      241 gggctgctgg cgatgcttgc ccgcgcctat ggtgtttctc ccagcagcag atgaatcgag
      301 aaagcctgcg cccaaggggg tgaggtcgca tcatcgcgct tcagatccat cgacgaatac
      361 ggtttaaggg gaattggtgt gactagacaa cgctctctgc tgacgcctgg tccattgtcg
      421 ctgtcgctgg cgggtcagga gccagatgct gctcgacctc gcatcgcgcg atggtgagtt
      481 caaggaagtg accgcccgca tgaggcggct gatgctcaat ctgctgggag gtgccaagga
      541 ctattcggtg gtccccattc aggggggcgg gtcctttgca atggaagccg cgcgctcttc
      601 attcgtgtcc aggaacgaca agccgctcgt ttgcataaac ggcatctatg gcgagcgcat
      661 tttcaagatt ctgcggctgt ggggcgtcga agcgctgaag cttgtcaagc gagcgaccga
      721 gcccctggac ccccaggaag ttgccgagca gctgagccga aatcccggcg ttactcacct
      781 atgtcttgtg cattgcgaga caacaaccgg aatcgtcaat ccgcttgacg cgatcataga
      841 ggaggcgagg cggcgtggtg tgaagacgat cgtcgacggc atgagctctt ttggcgcgat
      901 tgacatcgac ctaagccgtg gtggaccgga tgtcctggtc acatcgagca ataagtgcat
      961 cgaagggccg ccaggactag cttttgtcat cgcgtctcgc gagctgctgg aaaaagcggt
     1021 tcaagaacca aggtcatttg tgctcgacgt cacacaccaa tggctctcgc tcgagcgtac
     1081 cggagagtgg cgatcgaccc cacccaccca catcgttcag gcaacaacga aggctttgga
     1141 gattctggag ggggagggca ttgatgccag gcgccgcagg tatgagaagg tcagagacga
     1201 tctcgtccaa gaactcgaag gggtagtgtc tccgctgcta tccgccgaat tgcagtctcc
     1261 ggtctgcgtc gcgttcagcg cgccgtccgg aatcgcggat caggcaggct tcgatgggct
     1321 atatcgtcac ttggcggccc acaatcttta tatctactcg aagctgcatc ttgcgacgcg
     1381 gagttttcgg gtcggctgca ttggcgagat ccagcccagt tggatcgagc agttggggtg
     1441 cgcctttcgt acatattttc ggtccggccc ggcttcatcc atggggacgc cgtcaacccg
     1501 ccaggcctgc gagcgtgggg tcgagatgtc atcgtccttc gcaaaggact cgcagctgcc
     1561 gttctctgcc gaaactgctg ttctgcacgc cggctaccga cgcgatccgg tgacgaaagc
     1621 tgtcgcagtg ccgatctatc agaacacggc ttacgaactt gatggcgatc tgaatcacat
     1681 tgcggacgtc tatacgtcaa ggctgatgga ttcacctata cgaggatcat caacccgacg
     1741 acccgcgcgc tggaaaaaag gtatgccgcg gtcgacatgg gaagcgactc gctcgccgtc
     1801 gcatcaggtc aagcggcaac cttccttgcc atcctcaacc tgtcaagcgg cgaggtgggg
     1861 gacaatatcg tcgcctcacc gtatctctat ggcaacacgt ggaatctgct ccataacacg
     1921 ctgaagcgtc ttggtatcag cgtcagaacg gcagaccccc gaaggcccga gaccttcgaa
     1981 cgtgccatcg atgatcgcac gatctgcctg ttcggagagg tgatttcaaa tccttgcctg
     2041 attccgcttc cggtcaaaca gctggctgag atcggccgaa agcacggcgt tccgttggtg
     2101 gtggataata cgacgacccc gctggtatgt cggccgtcag atctcggtgc tgcgattacg
     2161 acctactccg ctacgaaata catatgcggc catggcacaa cgctgggcgg tctgatcgtt
     2221 gacaacggca agttcagcta ccgcggcgcc tctcgctttc ccttgttcaa cagtcctgac
     2281 gaggcgcatg gcgggatcat ctggcgcaac gcgctgcaag atgtcgacga tctcggaaaa
     2341 agcgaggttc tcttgaaggc tcgcatgacc tggttgcgcg atactggcgc ggccattgcc
     2401 ccctttgcga gctttcagct gatccaaggc cttgaaacgc tgccccttcg catgaagcag
     2461 cactgcgcaa acgccaggat cgtggccgac gttcttaagg agcatccaaa agtgcgccgc
     2521 gtcttctacc cggggctgtt cgagggagcc gatcgggaaa ctgtcgacca gacactcaat
     2581 cccgcatacg gacacggcgc gatggtcatg ttcgaagttg aggacgagca ggccgggcgg
     2641 aaattcatcc agaatgtcga cctgatgtat cacgtctcga atgtcgggga cgcccgtacg
     2701 ctcgtaacgc atcctgtttc gaccacccac accactgttc cgcgggagaa gcgcgaaccg
     2761 ccggcatatt tggcggctcg atccgacttt gcgtgggcat cgaagatgtc gacgacatcg
     2821 tgcgcgatct ggacagggcg ctttccgcaa tctgacaact tgtaaggcga tcaggaggag
     2881 gttctcatga atcaggcaga cgcttggaaa ctgaggtcgt ctcgttatga ggctgttcag
     2941 ttcagcc
//