From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: S.flexneri glpX gene.
Date: 12 Sep 92 10:03:40 GMT
Distribution: bionet
Checksum: 55041     5

LOCUS       SHFGLPXG     1748 bp ds-DNA             BCT       12-SEP-1992
DEFINITION  S.flexneri glpX gene.
ACCESSION   Z11766
KEYWORDS    ORF1; glpFK operon; glpX gene.
SOURCE      Shigella flexneri DNA.
  ORGANISM  Shigella flexneri
            Prokaryotae; Gram-negative facultatively anaerobic rods;
            Enterobacteriaceae.
REFERENCE   1  (bases 1 to 1748)
  AUTHORS   Truniger,V.
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
REFERENCE   2  (bases 1 to 1748)
  AUTHORS   Truniger,V., Sweet,G. and Boos,W.
  TITLE     glpF mutants cause reduced membrane permeability in Escherichia
            coli
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
REFERENCE   3  (bases 1 to 1748)
  AUTHORS   Truniger,V., Sweet,G. and Boos,W.
  TITLE     glpX, a new glp gene is located at the end of the glpFK operon of
            Escherichia coli
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
COMMENT     *source: strain=M4243; *source: clone=glpF; *source:
            is_macronuclear=N; *source: is_proviral=N; *source: is_germline=N;
            From EMBL    entry SFGLPXG;  dated 03-SEP-1992.
FEATURES             Location/Qualifiers
     repeat_unit     42..76
                     /note="repetitive element"
     repeat_unit     95..128
                     /note="repetitive element"
     CDS             235..1245
                     /function="unknown"
                     /note="third genein glpFKX operon"
                     /gene="glpX"
                     /codon_start=1
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGGVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     terminator      1266..1294
     CDS             1342..>1748
                     /note="product has unknown function"
                     /gene="ORF1"
                     /codon_start=1
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEQLASLFWMK
                     YRTAKRYGCWQPVQRLALIYRFCNLGKDLDRFK"
BASE COUNT      414 a    463 c    492 g    379 t
ORIGIN
        1 gagtaatgtc aggcacctga atcgattgcc tgatgctgat cgcctgatgc gacgttacgc
       61 gtcttatcag gcctacgagc caatgccatt ttgtgtaggc gggataaggc gttcacgccg
      121 catccggcat cacgcagact ctgaaaccgt gcgggggcaa ccccgcacac atcaataatc
      181 ccacccttcc cctgtgctac acttcgcgcc attccttact gcttagagtt tgctatgaga
      241 cgagaacttg ccatcgaatt ttcccgcgtc accgaatctg cggcgctggc tggctacaaa
      301 tggttaggac gcggcgataa aaacaccgcg gacggcgcgg cggtaaacgc catgcgtatt
      361 atgctcaacc aggtcaacat tgacggcacc atcgtcattg gtgaaggtga aatcgacgaa
      421 gcaccgatgc tctacattgg tgaaaaagtc ggtactggtc gcggcgacgc ggtagatatt
      481 gctgttgatc cgattgaagg cacgcgcatg acggcgatgg gccaggctaa cgcgctggcg
      541 gtgctggcag taggcgataa aggctgcttc ctcaatgcgc cggatatgta tatggagaag
      601 ctgattgtcg ggccgggagc caaaggcacc attgatctga acctgccgct ggcggataac
      661 ctgcgcaatg tagcggcggc gctcggcaaa ccgttgagcg aactgacggt aacgattctg
      721 gctaaaccac gccacgatgc cgttatcgct gaaatgcagc aactcggcgt acgcgtattt
      781 gctattccgg acggcgacgt tgcggcctca attctcacct gtatgccaga cagcgaagtt
      841 gacgtgctgt acggtattgg tggcgcgccg gaaggcggag tttctgcggc ggtgatccgc
      901 gcattagatg gcgacatgaa cggtcgtctg ctggcgcgtc atgacgtcaa aggcgacaac
      961 gaagagaatc gtcgcattgg cgagcaggag ctggcacgct gcaaagcgat gggcatcgaa
     1021 gccggtaaag tattgcgtct gggcgatatg gcgcgcagcg ataacgtcat cttctctgcc
     1081 accggtatta ccaaaggcga tctgctggaa ggtattagcc gcaaaggcaa tatcgcgact
     1141 accgaaacgc tgctgatccg cggcaagtca cgcaccattc gccgcattca gtccatccac
     1201 tatctggatc gcaaagaccc ggaaatgcag gtgcacattc tctgagtgat ttgatcgatt
     1261 gagccttcca gtccttcggg actggaattt ttttgttcgg agaacgaaga taaggcaagt
     1321 caatcaaaac aggagaaaaa catggctgat tgggtaacag gcaaagtcac taaagtgcag
     1381 aactggaccg acgccctgtt tagtctcacc gttcacgccc ccgtgcttcc gtttaccgcc
     1441 gggcaattta ccaagcttgg ccttgaaatc gacggcgaac gcgtccagcg cgcctactcc
     1501 tatgttaact cgcccgataa tcccgatctg gagttttacc tggtcaccgt ccccgatggc
     1561 aaattaagcc cacgattggc ggcactgaaa ccgggcgatg aagtgcaggt ggttagcgaa
     1621 cagctggctt ctttgttctg gatgaagtac cggactgcga aacgctatgg atgctggcaa
     1681 ccggtacagc gattggccct tatttatcga ttctgcaacc taggtaaaga tttagatcgc
     1741 ttcaaaaa
//