HDTenEightyP/NetNews huggingface dataset:
From: fgarbrec@POST.ITS.MCW.EDU (GARBRECHT)
Newsgroups: bionet.immunology
Subject: Re: ER - resident proteins
Date: 17 Dec 92 23:35:39 GMT
Distribution: bionet

On 16 Dec 1992, M.D. O'Leary wrote:

> Date: 16 Dec 92 15:24:53 GMT
> From: M.D. O'Leary <Mark.OLeary@newcastle.ac.uk>
> To: immunology@net.bio.net
> Subject: ER - resident proteins
> 
> Hi,
> 
>    I am trying to visualise ER-resident proteins (B1P etc) on an SDS-PAGE 
> immunologically. I have tried lysed mammalian (hybridoma) cells, but got no 
> detectable signal.
> 
>    If anyone who has tried to do something similar, or has any suggestions 
> (such as a method to enhance ER-resident protein levels in the applied sample?)
> I would be most grateful if they would contact me on mdo4@le.ac.uk.
> 
> PS. If anyone had another antibody raised against an ER-resident protein, that
> would also be useful to me as a control...
> 
> I hope someone will be able to help me...
> 
> Mark
> 
I don't have experiance with your specific problem (ER proteins), but
perhaps if
you could tell us a little more about your experimental conditions and
reagents, I (or someone else) could help pinpoint the problem, or at
least offer suggestions.

Newsgroups: bionet.immunology,bionet.molbio.evolution
From: ecec@quads.uchicago.edu (Eric Cabot)
Subject: Re: Evolution of the immune system
Reply-To: ecec@midway.uchicago.edu
Organization: University of Chicago Computing Organizations
Date: Fri, 11 Dec 1992 21:00:26 GMT

In article <1992Dec11.153054.22186@midway.uchicago.edu> ecec@midway.uchicago.edu writes:
>In article <92345.091058FORSDYKE@QUCDN.QueensU.CA> <FORSDYKE@QUCDN.QueensU.CA> writes:
>>Larry says (ref.1)
>>>
>>    It does not follow that because many genes which are functionally related
>>are not linked, that functional relationships never require close linkage.
>>One obvious example are meiotic drive loci, often within inversions, where the
>>drive gene and its target are closely linked. If two proteins must be finely
>
>Please allow me to stick my oar in here a moment.  I don't really agree
>that the participants in meiotic drive loci are really as tightly linked
>as you imply.  Take the Drosophila melanogaster SD system, for example,
>the Sd and responder loci are on different sides of the centromere
>and linkage disequilibrium between the two has not been demonstrated.
>Sd and rsp can be separated by recombination with reasonable ease
>(but with dire consequences for the Sd bearing chromosome).
>Furthermore, these loci are not bound up by an inversion. Think
>of the consequences if they were!  There are often inversions associated
>with some drive loci, but this might be a case of hitch-hiking.
>
>
>-- 
As a followup to my earlier posting (shown above) I'd like to clarify that
the major players in the segregation distorter system are linked
but not necessarily because of fine-tuning of the gene products.
More likely the linkage is necessary to ensure that chromosomes
with Sd will not easily loose the insensitive responder allele (and 
gain a sensitive allele) by recombination.  The looser the linkage,
presumably, the harder it would be for SD chromosomes to persist.
A pericentric inversion  occurring on an SD chromosome
would tighten this linkage and would probably have some fitness advantage
over other SD chromosomes which could become sensitive to drive 
(that is "suicidal") as a result of recombination. 

I'd also like to note that there are in fact several known examples
of SD chromosomes that have the Sd and responder loci bound
up within inversions (SD-Roma, for example).  In otherwords,
I was incorrect in my initial posting.  That's what I get for
posting to the net before having my first cup of coffee!


-- 
=+=+=+=+=+=+=+=+=+=+=+=+=+=+=+=+=+=+=+=v=+=+=+=+=+=+=+=+=+=+=+=+=+=+=+=+=+=+=
    Eric Cabot                         |       "Non Nobis Nati Solum" 
ecec@midway.uchicago.edu               | 
=+=+=+=+=+=+=+=+=+=+=+=+=+=+=+=+=+=+=+=v=+=+=+=+=+=+=+=+=+=+=+=+=+=+=+=+=+=+=
From: p_travers@icrf.icnet.uk
Newsgroups: bionet.immunology
Subject: looking for h2-Kd cDNA clone
Date: 13 Dec 92 14:05:31 GMT
Organization: Imperial Cancer Research Fund, London, UK
NNTP-Posting-Host: bonnie.lif.icnet.uk

Does anyone have an H2-Kd cDNA clone that they would be willing to send me?
We are interested in peptide binding to MHC alleles and it turns out that Kd
is best suited for our purposes

			Paul Travers
				
			    Dept of Crystallography
			    Birkbeck College, London
			    P_TRAVERS@ICRF.ICNET.UK
From: q00023@TIGGER.STCLOUD.MSUS.EDU
Newsgroups: bionet.immunology
Subject: How to start insect cell culture?
Date: 15 Dec 92 17:47:23 GMT
Distribution: bionet

Dear netters,

	If anyone can tell me how to start insect cell culture. I am looking
for some methodologies of insect cell culture. Any insect cell lines will be
fine as long as they are easy to find. Please let me know if you have
information about that. Thank you very much.

Thanks in advance,

Gabriel Cheung
St.Cloud State Unversity
Xref: sparky bionet.immunology:191 bionet.molbio.evolution:436
Organization: Queen's University at Kingston
Date: Sun, 13 Dec 1992 11:00:18 EST
From: <FORSDYKE@QUCDN.QueensU.CA>
Newsgroups: bionet.immunology,bionet.molbio.evolution
Subject: Re: Evolution of the immune system
 <92345.091058FORSDYKE@QUCDN.QueensU.CA>
 <1992Dec11.153054.22186@midway.uchicago.edu>
 <1992Dec11.210026.691@midway.uchicago.edu>

In article <1992Dec11.210026.691@midway.uchicago.edu>, ecec@quads.uchicago.edu
(Eric Cabot) says:
>
>In article <1992Dec11.153054.22186@midway.uchicago.edu>                       u
>ecec@midway.uchicago.ed
>writes:
>>In article <92345.091058FORSDYKE@QUCDN.QueensU.CA>
><FORSDYKE@QUCDN.QueensU.CA>
>writes:
>>>Larry says (ref.1)
>>>>
>>>    It does not follow that because many genes which are functionally
>related
>>>are not linked, that functional relationships never require close linkage.
>>>One obvious example are meiotic drive loci, often within inversions, where  e
>th
>>>drive gene and its target are closely linked. If two proteins must be finely
   ..............

>>Please allow me to stick my oar in here a moment.  I don't really agree
>>that the participants in meiotic drive loci are really as tightly linked
>>as you imply.  Take the Drosophila melanogaster SD system, for example,
>>the Sd and responder loci are on different sides of the centromere......

>As a followup to my earlier posting (shown above) I'd like to clarify that
>the major players in the segregation distorter system are linked
>but not necessarily because of fine-tuning of the gene products.
>More likely the linkage is necessary to ensure that chromosomes
>..........
>I'd also like to note that there are in fact several known examples
>of SD chromosomes that have the Sd and responder loci bound
>up within inversions (SD-Roma, for example).  In otherwords,
>I was incorrect in my initial posting.  That's what I get for
>posting to the net before having my first cup of coffee!
>    Eric Cabot                         |       "Non Nobis Nati Solum"
>ecec@midway.uchicago.edu               |

    Thanks for your contribution anyway. We seem to have wandered somewhat off
the topic since Larry Moran puckishly reminded us that we should be careful
not to necessarily regard modern organisms as "living fossils".
                                   Sincerely,  Don Forsdyke
From: smith-una@YALE.EDU (Una Smith)
Newsgroups: bionet.announce
Subject: Ecology Gopher
Summary: New host:  gopher.yale.edu
Date: 16 Dec 92 17:15:18 GMT
Organization: Yale University Science & Engineering UNIX(tm), New Haven, CT 06520-2158


The Ecological Data Exchange has moved a little bit.  Below is the
gopher link data, for gopher administrators to pop into their files.
Other users can reach the EDEX directly from Unix and other computers
using the command

        gopher gopher.yale.edu

and then navigating through the menu items Enterprise to Academic
to Forestry.  The EDEX administrator would welcome comments and
questions:  send them to goforest@gopher.yale.edu.  Current contents
of the data base include data sets for many hardwood forests in
New England, including some Hubbard Brook permanent plot data sets.


Name=Ecological Data Exchange at Yale
Type=1
Host=gopher.yale.edu
Port=70
Path=1/Enterprise/Academic/Forestry

mail to :  goforest@gopher.yale.edu

---------------------
Send all comments to goforest@gopher.yale.edu, not me, please.
-- 

      Una Smith      Biology Department       smith-una@yale.edu
                     Yale University
                     New Haven, CT  06511
From: POSTMASTER@NBRF.Georgetown.Edu
Newsgroups: bionet.announce
Subject: Announcement of PIR Service Interruption
Date: 17 Dec 92 15:12:54 GMT


            Announcement of Temporary Interruption of Access to
                   The Protein Identification Resource

The National Biomedical Research Foundation will be attempting to repair a
hardware problem on Monday 21 December.  From 10:00 EST Monday 21 December
through about 12:00 EST Tuesday 22 December it will not be possible for users
to access either the Protein Identification Resource Network Server or the
On-line System.

We regret any inconvenience this temporary loss of service will cause.
------------------------------------------------------------------------
                                 Dr. John S. Garavelli
                                 Database Coordinator
                                 Protein Information Resource
                                 National Biomedical Research Foundation
                                 Washington, DC  20007
                                 POSTMAST@GUNBRF.BITNET
                                 POSTMASTER@NBRF.GEORGETOWN.EDU
From: kristoff@net.bio.net (David Kristofferson)
Newsgroups: bionet.announce
Subject: bionet.virology and bionet.journals.note are ready
Date: 16 Dec 92 23:13:12 GMT
Distribution: bionet
Organization: BIOSCI International Newsgroups for Biology

The new newsgroups bionet.virology and bionet.journals.note are ready
for operation on USENET.  The corresponding e-mail lists are also
ready and the addresses are as follows:

Addresses for all e-mail subscription requests:

biosci@net.bio.net 		(Americas and Pacific Rim)
biosci@daresbury.ac.uk		(Europe, Africa, and Central Asia)

Please do NOT send subscription requests to the newsgroup posting
addresses!!

The e-mail posting addresses are

for bionet.virology:		virology@daresbury.ac.uk
				virology@net.bio.net

for bionet.journals.note:	jrnlnote@daresbury.ac.uk
				jrnlnote@net.bio.net

USENET users simply ready and post directly to the USENET newsgroups
themselves.

The discussion leaders will post their introductory notes to the
groups fairly soon.  Donald Forsdyke is leading JRNLNOTE and Robert
Coelen is handling VIROLOGY.  Robert may be out of town at the moment
so it is possible that he may not be able to respond for a couple of
weeks.

				Sincerely,

				Dave Kristofferson
				BIOSCI/bionet Manager

				kristoff@net.bio.net
Newsgroups: bionet.neuroscience
From: eddy@boulder.Colorado.EDU (Sean Eddy)
Subject: Re: Neurotransmitters in C. elegans
Organization: University of Colorado, Boulder
Date: 17 Dec 92 14:28:19 GMT

khofmann@biomed.biolan.uni-koeln.de writes:
> Can anybody tell me, what kind of neurotransmitters are used in the worm
>Caenorhabditis elegans? This species has to my knowledge (but I am no
>neuroscientist) a small but fairly developed nervous system. But I was not
>able to track down any information on the neurotransmitters used in 
>C.elegans synapses.

A good starting reference would be M. Chalfie and J. White's
chapter in _The Nematode Caenorhabditis elegans_, W.B. Wood (ed.), 
1988, Cold Spring Harbor Laboratory Press.

It's thought that excitatory body-wall muscle motor neurons
use acetylcholine. Inhibitory motor neurons use GABA. 

Excitatory motor neurons in the egg-laying circuitry seem
to be using serotonin.

Dopamine and octopamine have been detected in the worm as
well.

- Sean Eddy
- MRC Laboratory of Molecular Biology
- sre@mrc-lmb.cam.ac.uk

Newsgroups: bionet.neuroscience
From: winstel@kit.uni-kl.de (Rainer Winstel)
Subject: Neurotrophins
Organization: Universitaet Kaiserslautern
Date: Mon, 14 Dec 1992 19:32:18 GMT

I'm interested in the function of neurotrophins in the development of the 
nerve system.  Does anyone have any information about this, particularly 
about CNTF, CDF and IL-6
Thanks in advance,
Rainer Winstel
Newsgroups: bionet.neuroscience
From: olds@helix.nih.gov (James Olds)
Subject: Re: biochem of learning
Organization: National Institutes of Health, Bethesda
Date: Thu, 17 Dec 1992 21:19:49 GMT

In article <1992Dec17.162305.23462@midway.uchicago.edu> kps2@midway.uchicago.edu writes:
>
>I would strongly recommend 
>Byrne, J. H. (1987) Cellular Analysis of Associative Learning,
>Physiological Reviews, 87:329-439. 
>to anyone interested in this subject.
>-- 
>Ken Scholz                    Department of Pharm. and Physiology
>kps2@midway.uchicago.edu      Univ. of Chicago

I would even more strongly recommend Dan Alkon's new book
Memory's Voice which is just out in book stores and is published
by Harper Collins. It is an excellent synthesis of the field 
with a superb historical background.
 
--
****************************************************************************
* James L. Olds Ph.D.                 Neural Systems Section               *
* domain: olds@helix.nih.gov           NINDS, NIH, Bethesda, MD. 20892 USA *
****************************************************************************
From: smith3682@iscsvax.uni.edu
Newsgroups: bionet.neuroscience
Subject: biochem of learning
Date: 16 Dec 92 17:02:08 -0600
Organization: University of Northern Iowa

I'm begining a study about the role played by second messengers in learning
behavior.  In particular, I would like to know more about current models being
developed n cell responses to various stimuli, and the way these responses lead
to long-term changes in the cell.  I am also interested in the way certain
drugs affect these cellular responses and ultimately affect learning and 
memory.  Does any one know any good, recent references.
Newsgroups: bionet.neuroscience
From: khofmann@biomed.biolan.uni-koeln.de
Subject: Neurotransmitters in C. elegans
Reply-To: khofmann@biomed.biolan.uni-koeln.de
Organization: Biochemical Institute, Cologne University
Date: Thu, 17 Dec 92 11:07:00 GMT

Dear neuroscientific community,
 Can anybody tell me, what kind of neurotransmitters are used in the worm
Caenorhabditis elegans? This species has to my knowledge (but I am no
neuroscientist) a small but fairly developed nervous system. But I was not
able to track down any information on the neurotransmitters used in 
C.elegans synapses

Thank you in advance,
                        Kay Hofmann
Newsgroups: bionet.neuroscience
From: olds@helix.nih.gov (James Olds)
Subject: Re: Biochem of LTP (was Re: biochem of learning)
Organization: National Institutes of Health, Bethesda
Date: Sat, 19 Dec 1992 14:15:49 GMT

In article <1gv64lINNh8b@neuro.usc.edu> merlin@neuro.usc.edu (merlin) writes:
>If you believe LTP (long term potentiation is related to learning
>and memory) then you should take a look at:
>
>Kuba-K & Kumamoto-E.  Long-term potentiation in vertebrate synapses:
>a variety of cascades with common subprocesses.  Progress in
>Neurobiology 34:197-269 (1990).
>
>Baudry-M.  "An integrated biochemical model for long-term potentiation."
>Long-Term Potentiation: A Debate of Current Issues.  Baudry-M & Davis-JL
>(eds).  MIT Press 1991.  ISBN 0-262-02330-X.
>

...and for those of us who don't :-) ????
 Seriously, it worries me that there is a quasi-religious belief
 floating around in the field that LTP is the same thing as
 learning. While I have no doubt that these two phenomena (one 
 produced by the experimenter, the other prooduced by nature) do
 in fact share certain biochemical and biophysical characteristics,
 I also havee a hunch that there are certain aspects of LTP which
 are non-related to real memory formation (and I guess therefore
 could be called artifactual). That is why, I am a big fan of
 studying real learning and memory by....
 yes, studying the real phenomenon. That way the issues don't gete
 confused. We aren't left to decipher what was and what wasn't part
 of the real McCoy.

 Historically, the critisisms for my point of view tended to
 revolve around the idea that with the "study learning to understand
 learning" approach, it would be impossible to get molecular...thee
 system was too complicated. However, history (i.e. 30 years of
 research in this area) has shown that this critism doesn't hold
 water.

 Flame on (grin)


--
****************************************************************************
* James L. Olds Ph.D.                 Neural Systems Section               *
* domain: olds@helix.nih.gov           NINDS, NIH, Bethesda, MD. 20892 USA *
****************************************************************************
From: drayer-rebecca@yale.edu (Rebecca Drayer)
Newsgroups: bionet.neuroscience
Subject: Re: biochem of learning
Date: 16 Dec 1992 18:42:31 -0500
Organization: Yale University Science & Engineering UNIX(tm), New Haven, CT 06520-2158
NNTP-Posting-Host: minerva.cis.yale.edu

In article <1992Dec16.170208.9461@iscsvax.uni.edu> smith3682@iscsvax.uni.edu writes:
>I'm begining a study about the role played by second messengers in learning
>behavior.  In particular, I would like to know more about current models being
>developed n cell responses to various stimuli, and the way these responses lead
>to long-term changes in the cell.  I am also interested in the way certain
>drugs affect these cellular responses and ultimately affect learning and 
>memory.  Does any one know any good, recent references.

I'd try _Principles of Neural Science_, that huge book by Kandel,
Schwartz, and Jessel.  The last few chapters deal a great deal with
learning and memory, and talk about the second messenger systems involved
in presynaptic facilitation in Aplysia and LTP in the hippocampus.
-- 
Rebecca A. Drayer, EMT-A            |    drayer@minerva.cis.yale.edu
Neurobiology major                  |    Computing Assistant
                                    |    Silliman College, Yale University
Newsgroups: bionet.neuroscience
Subject: New magazine - Psyche
From: x91007@phillip.edu.au
Date: 15 Dec 92 00:00:56 EST
Organization: RMIT (Bundoora Campus)

Announcement: New Electronic Magazine 'Psyche'.

I am interested in starting up a electronic magazine 
exploring broad issues relating to consciousness and the 
mind. I hope to explore these issues in an interdisciplinary 
way - using approaches and insights from psychology, 
philosophy, anthropology, computer science and mathematics.

Examples of some of the general areas that Psyche will cover 
are: the mind-body problem; altered-states-of-consciousness; 
psychoactive drugs; shamanism; dream states; hypnosis; 
artificial intelligence; meditative states; ecstatic 
religious states; blindsight; language and mind. 

In an attempt to offer a grounding in some of the central 
themes of Psyche, major review articles of influential 
ideas/books/movements will also be solicited. Examples of 
what these might be are: John Searle's Chinese Room Problem; 
Daniel Dennett's book 'Consciousness Explained'; Gordon 
Wasson's theories regarding Soma; Roger Penrose's book 'The 
Emperor's New Mind'.

While Psyche will not be peer-reviewed this does not mean 
that it will become a haven for New Age/Lunatic Fringe ideas. 
I have just completed a BSc Honours majoring in psychology, 
and I am currently studying the way the brain codes the 
position of objects. I would like to balance by skills with 
others and so am particularly interested in getting in 
contact with people with backgrounds in either computer 
science, philosophy or anthropology to act as sub/co-editors.

While the date is obviously tentative I would like to get the 
first issue of Psyche out by March 1993. It will be produced 
in ASCII and hopefully postscript. As tends to be the nature 
of electronic journals clarity, rather than length, will be a 
critical factor in deciding which articles are accepted.

So if your interested in: helping, supplying copy, 
subscribing (at least for the first edition), or simply 
offering advice please contact me directly at the address 
below. In your reply please let me know where you read about 
Psyche.

Yours sincerely,
			 Patrick Wilken

x91007@phillip.edu.au 

Newsgroups: bionet.neuroscience
From: kps2@ellis.uchicago.edu (kenneth paul scholz)
Subject: Re: biochem of learning
Reply-To: kps2@midway.uchicago.edu
Organization: University of Chicago Computing Organizations
Date: Thu, 17 Dec 1992 16:23:05 GMT


I would strongly recommend 
Byrne, J. H. (1987) Cellular Analysis of Associative Learning,
Physiological Reviews, 87:329-439. 
to anyone interested in this subject.
-- 
Ken Scholz                    Department of Pharm. and Physiology
kps2@midway.uchicago.edu      Univ. of Chicago
From: merlin@neuro.usc.edu (merlin)
Newsgroups: bionet.neuroscience
Subject: Biochem of LTP (was Re: biochem of learning)
Date: 19 Dec 1992 04:55:49 -0800
Organization: University of Southern California, Los Angeles, CA
NNTP-Posting-Host: neuro.usc.edu

If you believe LTP (long term potentiation is related to learning
and memory) then you should take a look at:

Kuba-K & Kumamoto-E.  Long-term potentiation in vertebrate synapses:
a variety of cascades with common subprocesses.  Progress in
Neurobiology 34:197-269 (1990).

Baudry-M.  "An integrated biochemical model for long-term potentiation."
Long-Term Potentiation: A Debate of Current Issues.  Baudry-M & Davis-JL
(eds).  MIT Press 1991.  ISBN 0-262-02330-X.

------------------------------------------------------------------------------
Alexander-James Annala
Principal Investigator
Neuroscience Image Analysis Network
HEDCO Neuroscience Building, Fifth Floor
University of Southern California
University Park
Los Angeles, CA 90089-2520
------------------------------------------------------------------------------
From: mgkjones@CSUVAX1.CSU.MURDOCH.EDU.AU (Mike Jones)
Newsgroups: bionet.genome.arabidopsis
Subject: Postdoctoral Fellowship
Date: 18 Dec 92 00:57:40 GMT
Distribution: bionet



Postdoctoral Research Fellowship
Plant Molecular Biology/Plant Virology


A postdoctoral position is available from February 1993 funded by
the Cooperative Research Centre for Legumes in Mediterranean
Agriculture (CLIMA).

CLIMA is located in Perth, Western Australia, and involves the
W.A. Department of Agriculture, University of Western Australia,
Murdoch University and CSIRO.  The position will be at Murdoch
University, in collaboration with the WA Department of Agriculture.

Duration: 3 years with possibility of extension to 5 years.

Salary:  about A$36,000, depending on experience.

Duties:  molecular research on Cucumber Mosaic Virus and Bean Yellow
Mosaic Virus, with the aim of engineering resistance in legumes 
(particularly lupin, the main grain legume grown in Australia).

Associated programmes include tissue culture and transformation of
lupin.

A full range of equipment for molecular research is available,
including computer analysis of sequences, an ABI automated DNA
sequencer and Genescan software, oligonucleotide synthesis etc.,
and glasshouse facilities for plant growth.

Applications are sought from plant molecular biologists/plant
molecular virologists.

Perth has a Mediterranean climate, with average daily sunshine of
9 hours.  Clean air, wide open spaces, beautiful beaches and good
sailing make Western Australia a great place to live!

Contact:  Prof. MGK Jones, School of Biological and Environmental
Sciences, Perth, Western Australia, 6150.
Fax: +61-9-310-3505, Email:  mgkjones@murdoch.edu.au



Newsgroups: bionet.genome.arabidopsis
From: snd@mace.cc.purdue.edu (Saeid Nourizadeh)
Subject: Thank you for the inf. regarding the arab. meeting
Organization: Purdue University
Distribution: na
Date: Tue, 15 Dec 1992 18:02:31 GMT

  I would like to thank all who have respond to my question with regard
  to Arabidopsis meeting at OSU.


  Saeid Nourizadeh