From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: A.niger amyB gene for alpha-amylase
Date: 3 Jan 93 22:03:26 GMT
Distribution: bionet
Checksum: 00394 7

LOCUS       ASNAMYB      2443 bp ds-DNA             PLN       21-DEC-1992
DEFINITION  A.niger amyB gene for alpha-amylase
ACCESSION   X52756
KEYWORDS    alpha-amylase.
SOURCE      Aspergillus niger DNA.
  ORGANISM  Aspergillus niger
            Eukaryota; Fungi; Eumycota; Ascomycotina; Plectomycetes;
            Eurotiales; Trichocomaceae.
REFERENCE   1  (bases 1 to 2443)
  AUTHORS   Korman,D.R., Bayliss,F.T., Barnett,C.C., Carmona,C.L., Kodama,K.H.,
            Royer,T.J., Thompson,S.A., Ward,M.A., Wilson,L.J. and Berka,R.M.
  TITLE     Cloning, characterization, and expression of two alpha-amylase
            genes from Aspergillus niger var. awamori
  JOURNAL   Curr. Genet. 17, 203-212 (1990)
  STANDARD  full automatic
COMMENT     *source: strain=var.awamori, UVK143f;
               From EMBL    entry ANAMYB;  dated 01-DEC-1992.
FEATURES             Location/Qualifiers
     mat_peptide     join(264..368,424..462,548..663,733..841,910..1138,
                     1197..1359,1425..1571,1637..1877,1957..2241)
                     /function="hydrolysis ofalpha-1,4-glucosidic bonds in
                     polyglucosans"
                     /EC_number="3.2.1.1"
                     /product="alpha-amylase"
                     /gene="amyB"
                     /codon_start=1
                     /note="1,4-alpha-D-glucan4-glucanohydrolase"
     CAAT_signal     11..13
     TATA_signal     101..106
     sig_peptide     201..263
                     /gene="amyB"
                     /note="alpha-amylase"
     exon            201..368
                     /number=1
     intron          369..423
                     /number=1
     exon            424..462
                     /number=2
     intron          463..547
                     /number=2
     exon            548..663
                     /number=3
     intron          664..732
                     /number=3
     exon            733..841
                     /number=4
     intron          842..909
                     /number=4
     exon            910..1138
                     /number=5
     intron          1139..1196
                     /number=5
     exon            1197..1359
                     /number=6
     intron          1360..1424
                     /number=6
     exon            1425..1571
                     /number=7
     intron          1572..1636
                     /number=7
     exon            1637..1877
                     /number=8
     intron          1878..1956
                     /number=8
     exon            1957..2244
                     /number=9
     polyA_site      2336..2341
     CDS             join(201..368,424..462,548..663,733..841,910..1138,
                     1197..1359,1425..1571,1637..1877,1957..2244)
                     /function="hydrolysis of alpha-1,4-glucosidicbonds in
                     polyglucosans"
                     /EC_number="3.2.1.1"
                     /gene="amyB"
                     /note="1,4-alpha-D-glucan 4-glucanohydrolase"
                     /product="alpha-amylase-precursor"
                     /codon_start=1
                     /translation="MMVAWWSLFLYGLQVAAPALAATPADWRSQSIYFLLTDRFARTD
                     GSTTATCNTADQKYCGGTWQGIIDKLDYIQGMGFTAIWITPVTAQLPQTTAYGDAYHG
                     YWQQDIYSLNENYGTADDLKALSSALHERGMYLMVDVVANHMGYDGAGSSVDYSVFKP
                     FSSQDYFHPFCFIQNYEDQTQVEDCWLGDNTVSLPDLDTTKDVVKNEWYDWVGSLVSN
                     YSIDGLRIDTVKHVQKDFWPGYNKAAGVYCIGEVLDGDPAYTCPYQNVMDGVLNYPIY
                     YPLLNAFKSTSGSMDDLYNMINTVKSDCPDSTLLGTFVENHDNPRFASYTNDIALAKN
                     VAAFIILNDGIPIIYAGQEQHYAGGNDPANREATWLSGYPTDSELYKLIASRNAIRNY
                     AISKDTGFVTYKNWPIYKDDTTIPMRKGTDGSQIVTILSNKGASGDSYTLSLSGAGYT
                     AGQQLTEVIGCTTVTVGSDGNVPVPMAGGLPRVLYPTEKLAGSKICSSS"
BASE COUNT      669 a    620 c    558 g    595 t      1 others
ORIGIN      
        1 ctgcagaatn aatttaaact cttctgcgaa tcgcttggat tccccgcccc tggccgtaga
       61 gcttaaagta tgtcccttgt cgatgcgatg tatcacaaca tataaatact agcaagggat
      121 gccatgcttg gaggatagca accgacaaca tcacatcaag ctctcccttc tctgaacaat
      181 aaaccccaca gaaggcattt atgatggtcg cgtggtggtc tctatttctg tacggccttc
      241 aggtcgcggc acctgctttg gctgcaacgc ctgcggactg gcgatcgcaa tccatttatt
      301 tccttctcac ggatcgattt gcaaggacgg atgggtcgac gactgcgact tgtaatactg
      361 cggatcaggt gtgttgttac ctactagctt tcagaaagag gaatgtaaac tgacttgata
      421 tagaaatact gtggtggaac atggcagggc atcatcgaca aggtaaattg cccctttatc
      481 aaaaaaaaag aaggaaaagc agaagaaaaa taaaataaaa agaactctag tcctaaccat
      541 cacatagttg gactatatcc agggaatggg cttcacagcc atctggatca cccccgttac
      601 agcccagctg ccccagacca ccgcatatgg agatgcctac catggctact ggcagcagga
      661 tatgtaagtc gatttcttta aatatctacc tgtcatcttt tacatcaata tgaactaact
      721 tgatggtttt agatactctc tgaacgaaaa ctacggcact gcagatgact tgaaggcgct
      781 ctcttcggcc cttcatgaga gggggatgta tcttatggtc gatgtggttg ctaaccatat
      841 ggttcgtggt cctttgcaac tgacttcgcg gatatggttc atttcagtac tgacaatgag
      901 taatatcagg gctatgatgg agcgggtagc tcagtcgatt acagtgtgtt taaaccgttc
      961 agttcccaag actacttcca cccgttctgt ttcattcaaa actatgaaga tcagactcag
     1021 gttgaggatt gctggctagg agataacact gtctccttgc ctgatctcga taccaccaag
     1081 gatgtggtca agaatgaatg gtacgactgg gtgggatcat tggtatcgaa ctactccagt
     1141 aagatatttc tccctcattc tacaacttgg ctgatcgatg atacttacga aatcagttga
     1201 cggcctccgt atcgacacag taaaacacgt ccagaaggac ttctggcccg ggtacaacaa
     1261 agccgcaggc gtgtactgta tcggcgaggt gctcgacggt gatccggcct acacttgtcc
     1321 ctaccagaac gtcatggacg gcgtactgaa ctatcccatg tatggttcct ccaaccatga
     1381 gccttcttgc aagtctcatc tcctaacgaa acggctaaaa ccagttacta tccactcctc
     1441 aacgccttca agtcaacctc cggcagcatg gacgacctct acaacatgat caacaccgtc
     1501 aaatccgact gtccagactc aacactcctg ggcacattcg tcgagaacca cgacaaccca
     1561 cggttcgctt cgtaagtctt cccttttatt ttccgttccc aatttccaca cagaacccca
     1621 cctaacaaga gcaaagttac accaacgaca tagccctcgc caagaacgtc gcagcattca
     1681 tcatcctcaa cgacggaatc cccatcatct acgccggcca agaacagcac tacgccggcg
     1741 gaaacgaccc cgcgaaccgc gaagcaacct ggctctcggg ctacccgacc gacagcgagc
     1801 tgtacaagtt aattgcctcc cggaacgcaa tccggaacta tgccattagc aaagatacag
     1861 gattcgtgac ctacaaggta agcacaacct ctaagcatac cctaatggcc tatcttcaga
     1921 gtatctgaca caagagacta atcactggca atacagaact ggcccatcta caaagacgac
     1981 acaacgatcc cgatgcgcaa gggcacagat gggtcgcaga tcgtgactat cttgtccaac
     2041 aagggtgctt cgggtgattc gtataccctc tccttgagtg gtgcgggtta cacagccggc
     2101 cagcaattga cggaggtcat tggctgcacg accgtgacgg ttggttcgga tggaaatgtg
     2161 cctgttccta tggcaggtgg gctacctagg gtattgtatc cgactgagaa gttggcaggt
     2221 agcaagatct gtagtagctc gtgaagggtg gagagtatat gatggtactg ctattcaatc
     2281 tggcattgga cagcgagatt gaatgtggtg gacgtaacct acgttgtgtc tgtagaatat
     2341 atacatgtaa gatacatgag cttcggtgat ataatacaga agtaccatac agtaccgcgt
     2401 tatggaatcg aactactaca gggcttttcc tataatagac tag
//