From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: X.campestris protease gene
Date: 3 Jan 93 22:22:52 GMT
Distribution: bionet
Checksum: 34538 6

LOCUS       XANPROTEA    2798 bp ds-DNA             BCT       23-DEC-1992
DEFINITION  X.campestris protease gene
ACCESSION   X51635
KEYWORDS    protease.
SOURCE      Xanthomonas campestris DNA.
  ORGANISM  Xanthomonas campestris
            Prokaryotae; Gracilicutes; Scotobacteria; Pseudomonadaceae.
REFERENCE   1  (bases 1 to 2798)
  AUTHORS   Liu,Y.N., Tang,J.L., Clarke,B.R., Dow,J.M. and Daniels,M.J.
  TITLE     A multipurpose broad host range cloning vector and its use to
            characterise an extracellular protease gene of Xanthomonas
            campestris pathovar campestris
  JOURNAL   Mol. Gen. Genet. 220, 433-440 (1990)
  STANDARD  full automatic
COMMENT     *source: clone=pIJ3074;
               From EMBL    entry XCPROTEA;  dated 15-DEC-1992.
FEATURES             Location/Qualifiers
     -35_signal      452..458
     -10_signal      495..499
     RBS             581..590
     CDS             592..2334
                     /product="protease"
                     /codon_start=1
                     /translation="MSTASLRKRTGSLTILGASALTSLLLAMPAFAGEVYLDGLATAQ
                     THQKFIVTYKDGSTALASPSALTTSLRTAARAVPAKAGKALGLNSVRRLALGPELVRA
                     DRALDRAEAETLMRQLAADPNVQSVEVDQILHATLTPNDTRLSEQWAFGTTNAGLNIR
                     PAWDKATGSGTVVAVIDTGITSHADLNANILAGYDFISDATTARDGNGRDSNAADEGD
                     WYAANECGAGIPAASSSWHGTHVAGTVAAVTNNTTGVAGTAYGAKVVPVRVLGKCGGS
                     LSDIADAIVWASGGTVSGIPANANPAEVINMSLGGGGSCSTTMQNAINGAVSRGTTVV
                     VAAGNDASNVSGSLPANCANVIAVAATTSAGAKASYSNFGTGIDVSAPGSSILSTLNS
                     GTTTPGSASYASYNGTSMASPHVAGVVALVQSVAPTALTPAAVETLLKNTARALPGAC
                     SGGCGAGIVNADAAVTAAINGGSGGGGGGGNTLTNGTPVTGLGAATGAELNYTITVPA
                     GSGTLTVTTSGGSGDADLYVRAGSAPTDSAYTCRPYRSGNAETCTITAPSGTYYVRLK
                     AYSTFSGVTLRASY"
BASE COUNT      463 a    953 c    917 g    465 t
ORIGIN      
        1 ctgcagctgg cccgtgaccg cggcatgcag gtggtggagc gcaaggtgtc catcgacgag
       61 tggaaggacg gcgtggcctc cggcgagatc agcgaggtgt tcgcctgcgg cacggcggct
      121 gtggttaccc cgatcggcga gctgaagggc gatggctttg cggtcggcga cctgtctgca
      181 ccggcgggcg aggtgacgat gtcgctgcgc caggagctga ccgacatcca gtacgggcgc
      241 gtgccggacc gccacggttg gctggtgcgt ctgtcctaag ccaacacgtc aagccaaatc
      301 agggcccctg cgcgtcagcg cagggctttt ttttgcgcgc agtgcagcaa gtgcgtgtca
      361 tttggtgtcg ccgcatgtca tcgcgagact gaacggtgcc gtacggtgtc acaaatctga
      421 tatttaattt tttgaaagtg cgaagttctt attgtccagt tcggttggat tgcggtaggt
      481 tcgctgaacg gatcgataac aggggcgatc cggggacctc ggcgctgacc tacggaaggc
      541 ggcgccggat aacaccgctc cggcggttct cttgatatgg aagggaattc gatgtcgact
      601 gcgtctctcc gcaagcgtac tggctcgctc accatcctgg gcgcgtccgc cctgacctcg
      661 ctgctgctcg cgatgccggc ctttgccggc gaggtctacc tggatggcct ggccaccgca
      721 cagacccatc agaaattcat cgtgacctac aaggacggca gcaccgcgct ggccagcccg
      781 tccgcgttga ccacctcgct gcgcactgct gcgcgcgcgg tgccggccaa agccggcaag
      841 gcgctgggcc tgaactcggt gcgccgcctg gcgttggggc cggaactggt aagggcagac
      901 cgcgccctgg accgcgccga ggccgaaacc ctgatgcggc aattggccgc tgatcccaac
      961 gtgcagagcg ttgaagtcga ccagatcctg catgccacgc tcacccccaa cgacacccgg
     1021 ttgtccgagc agtgggcgtt cggcaccacc aacgccggcc tgaacatccg cccggcctgg
     1081 gacaaggcca ccggcagcgg cacggtcgtg gcggtgattg ataccggcat caccagtcat
     1141 gccgacctca acgccaacat cctcgcgggc tacgacttca tcagcgatgc gaccaccgca
     1201 cgcgatggca acggccgtga cagcaacgcc gccgacgaag gcgactggta cgccgccaac
     1261 gaatgcggcg ccggcattcc cgccgccagc tccagctggc acggcaccca tgtggccggc
     1321 acggtcgcgg cagtgaccaa caacaccacc ggcgtagccg gcaccgccta cggcgccaag
     1381 gtcgtaccgg tgcgcgtgct cggcaagtgc ggtgggtcgc tgtcggatat cgccgacgcc
     1441 atcgtctggg cctccggcgg caccgtcagc ggcatcccgg ccaatgctaa cccggccgag
     1501 gtgatcaaca tgtcgctcgg cggcggcggt agctgctcga ccaccatgca gaacgccatc
     1561 aacggtgcgg tgtcgcgcgg caccacggtg gtggtcgcgg ccggcaacga tgcgtccaat
     1621 gtgtccggtt cgctgccggc caactgcgcg aacgtgattg cggtggccgc caccacctcg
     1681 gcgggcgcga aggccagcta ttccaacttc ggcaccggta tcgatgtgtc ggcgcccggc
     1741 tcgtcgatcc tgtccacgct caacagcggc accaccacgc cgggtagcgc cagctatgcc
     1801 tcctacaacg gcacctcgat ggcgtcgccg catgtggccg gcgtggtcgc gctggtgcag
     1861 tcggtcgccc cgaccgcgct gacgccagca gcggtggaaa ccttgttgaa gaacaccgcg
     1921 cgtgctttac cgggcgcctg ctcgggcggc tgcggtgccg gcatcgtcaa cgccgatgcc
     1981 gcggtcactg cggccatcaa tggcgggagc ggcggcggtg gcggtggtgg aaacaccttg
     2041 accaacggca ctccggtgac cggcctgggc gcggcgactg gcgcggaatt gaactacacc
     2101 atcaccgtgc cggccggcag cggcaccttg acggtgacca ccagcggcgg cagcggcgat
     2161 gccgacctgt atgtgcgcgc cggcagtgca ccgaccgact cggcttacac ctgccgccca
     2221 taccgcagcg gcaatgccga gacctgcacc atcaccgcac cgtccggaac gtattacgtg
     2281 cgtctgaagg cctacagcac gttctccggc gtcaccctgc gcgccagcta ctaaccgcgc
     2341 gtcacgcggg ccccggcact gcgccagggc gcgctcctca tctgcaatcc actccacgcc
     2401 tgccgattcg gcaggcgctg ggaatgtgtt gccgcgtggc cgggacggac accggtcagt
     2461 cgtttcgaca ggcacctgcc tgtgcacttc cagacctgcc atcgcagcgc gcgatggggt
     2521 ccggccatca cgccgcatct gcggcacctc atcactgaaa ggaacgatcg atgtccaatg
     2581 tgtcgcaacg tcgtgcgcct acgcgcgcgc tggtagtcct gggtgtgtcc gcgctgacct
     2641 cgctgctggc ggtgccggcc ttcgccgggc aggtgaatct gtccgggctg gattcgcagc
     2701 cgacgctgga tcgcttcatc gtcaaatacc gcgacggcag cagtgcgtcg gtgagcccga
     2761 cctcgatgcg cgcctcgctc aattcggttg gccactgc
//