From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human gene for elafin, complete cds.
Date: 6 Nov 92 18:06:31 GMT
Distribution: bionet
Checksum: 20621 5

LOCUS       HUMELAFIN    1878 bp ds-DNA             PRI       28-OCT-1992
DEFINITION  Human gene for elafin, complete cds.
ACCESSION   D13156
KEYWORDS    elafin; elastase inhibitor.
SOURCE      Homo sapiens (library: lambda EMBL) DNA, clone lambda hI-G1.
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 1878)
  AUTHORS   Saheki,T., Ito,F., Hagiwara,H., Saito,Y., Kuroki,J., Tachibana,S.
            and Hirose,S.
  TITLE     Primary structure of the human elafin precursor preproelafin
            deduced from the nucleotide sequence of it gene and the presence of
            unique repetitive sequences in the prosegment
  JOURNAL   Biochem. Biophys. Res. Commun. 185, 240-245 (1992)
  STANDARD  full automatic
COMMENT     Submitted (01-SEP-1992) to DDBJ by: Shigehisa Hirose Dept. of
            Biological Sciences Tokyo Institute of Technology Ookayama,
            Meguroku Tokyo 152 Japan Phone:  03-3726-1111 Fax:
            03-3729-0335.
FEATURES             Location/Qualifiers
     CAAT_signal     127..130
     TATA_signal     153..158
     exon            181..325
                     /number=1
     sig_peptide     247..324
                     /codon_start=1
     exon            1185..1460
                     /number=2
     mat_peptide     1286..1456
                     /product="elafin"
                     /codon_start=1
     exon            1695..1852
                     /number=3
     polyA_signal    1847..1852
     CDS             join(247..325,1185..1459)
                     /product="preproelafin"
                     /codon_start=1
                     /translation="MRASSFLIVVVFLIAGTLVLEAAVTGVPVKGQDTVKGRVPFNGQ
                     DPVKGQVSVKGQDKVKAQEPVKGPVSTKPGSCPIILIRCAMLNPPNRCLKDTDCPGIK
                     KCCEGSCGMACFVPQ"
BASE COUNT      473 a    464 c    491 g    450 t
ORIGIN      
        1 ctgtgagaaa gtaggaaaac tcttgggaca atcagagatg atgtgatgta atgtccatta
       61 gttcttcctg tgaataatcc tgagggaaag cccccaggtc cctcccagaa tggggtggat
      121 atttcccaat acagctaagg aattatccct tgtaaatacc acagacccgc ccctggagcc
      181 aggccaagct ggactgcata aagattggta tggccttagc tcttagccaa acaccttcct
      241 gacaccatga gggccagcag cttcttgatc gtggtggtgt tcctcatcgc tgggacgctg
      301 gttctagagg cagctgtcac gggaggtgag tgaacaggtg acctgctggg ctgggttgga
      361 ctaaggggag accctctggg acaccctggg ccaggacagg gagcactact gaagcagtag
      421 gcagcactgg agcccagatt tcagctttct gttctttgcc atcatattca gaaaaaatag
      481 gactttggct ggtggactcc acgtgctttc cacctcagtg actgagatat caggactgtt
      541 tgtggaagta atgttggtat gtggccttgg cctcagatgt caatacctgt gcagaatgtg
      601 caataaaata atgaactcca ggattttaaa ccttgggtgt ggacacagtc ccccgtttct
      661 ctgccccata aaagcactgg agtaatcagt actctaaaag gaggttaaga aacaacaagc
      721 cttcaggaat catgttgttt gaggaccccc attttataag gagggaacca aaaatgtaga
      781 aatgagtgag caattgccaa ggtaattccc agagccagga tggggctcaa gtctcctagt
      841 atgtggctca gggttctttc ctactccaat gcacttccta acaaatgaca atgtgtcctc
      901 ttcactgctg ggtgtcaccc cagtctgacc actgctcctg agagacttgg agtggaggaa
      961 gggggaagaa acaaatactc aagggaactc tggtcctgta gaccacccca aaaaaggaag
     1021 agccttccaa gagtgtagct cccagaggtg taccttccct actcaggcca tggtttgagg
     1081 atgctgcagt aagcagtgga tggacccaga cccagaggaa agacatggca gctgaagcag
     1141 aggcttactg ggtataaatg tgggctcgtt tcttctttta acagttcctg ttaaaggtca
     1201 agacactgtc aaaggccgtg ttccattcaa tggacaagat cccgttaaag gacaagtttc
     1261 agttaaaggt caagataaag tcaaagcgca agagccagtc aaaggtccag tctccactaa
     1321 gcctggctcc tgccccatta tcttgatccg gtgcgccatg ttgaatcccc ctaaccgctg
     1381 cttgaaagat actgactgcc caggaatcaa gaagtgctgt gaaggctctt gcgggatggc
     1441 ctgtttcgtt ccccagtgag gtgagcacta gctggagaac gaggagaccc ctgaagacac
     1501 aaaagaaggc tgagcggtgg ggaagcatcc caggttggtg ggagggaggt tgtgggaggt
     1561 gacagaaaga ctgggagagc tgaggggtct gagaggctat aaccagagtg cctagaagga
     1621 tgatctgtct tcctcactgc ctctgagtgc tttgatgtgc tgactctcac ctctgatact
     1681 cttctcttcc acagagggag ccggtccttg ctgcacctgt gccgtcccca gagctacagg
     1741 ccccatctgg tcctaagtcc ctgctgccct tccccttccc acactgtcca ttcttcctcc
     1801 cattcaggat gcccacggct ggagctgcct ctctcatcca ctttccaata aagagttcct
     1861 tctgctccac ttgtttct
//