From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Tomato BiP (binding protein)/grp78 (glucose-regulated protein, 78
Date: 28 Jan 93 05:49:15 GMT
Distribution: bionet
Checksum: 56084 7

LOCUS       TOMBIPGRBC   2232 bp ss-mRNA            PLN       27-JAN-1993
DEFINITION  Tomato BiP (binding protein)/grp78 (glucose-regulated protein, 78
            kD) (BiP/grp78) mRNA, complete cds.
ACCESSION   L08830
KEYWORDS    binding protein; glucose-regulated protein 78;
            heat shock protein 70; homologue.
SOURCE      Lycopersicon esculentum (cultivar VFNT Cherry) (library: cDNA
            library from Dr. B. Ishida, USDA PGEC Albany, CA.) cDNA to mRNA;
            Lycopersicon esculentum (strain UC82b) root cDNA to mRNA; and
            Lycopersicon esculentum (cultivar Castlemart) (library: ripe red
            fruit cDNA library from D. Della Pena, D.C. Alexander, & A. B.
            Bennet) cDNA to mRNA.
  ORGANISM  Lycopersicon esculentum
            Eukaryota; Plantae; Embryobionta; Magnoliophyta; Magnoliopsida;
            Asteridae; Solanales; Solanaceae.
REFERENCE   1  (bases 1 to 2232)
  AUTHORS   Meyer,D.J., Ewing,N.N., Rosichan,J.L. and Bennett,A.B.
  TITLE     Characterization of the tomato endoplasmic reticulum-localized
            BiP/GRP78 homolog
  JOURNAL   Unpublished (1993)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     5'UTR           1..119
                     /gene="BiP/grp78"
                     /evidence="EXPERIMENTAL"
                     /note="S1 nuclease protection and primer extension"
     sig_peptide     120..203
                     /gene="BiP/grp78"
                     /evidence="EXPERIMENTAL"
                     /codon_start=1
     mat_peptide     204..2117
                     /function="molecular chaperon"
                     /standard_name="BiP/grp78"
                     /product="glucose-regulated protein 78"
                     /gene="BiP/grp78"
                     /codon_start=1
     3'UTR           2121..2232
                     /gene="BiP/grp78"
     polyA_site      2232
                     /gene="BiP/grp78"
     CDS             120..2120
                     /function="molecular chaperon (precursor)"
                     /gene="BiP/grp78"
                     /note="an endoplasmic reticulum residing heat shock
                     protein 70 family member; precursor peptide"
                     /product="glucose-regulated protein 78"
                     /codon_start=1
                     /translation="MAACSRRGNSLVVLAIVLLGCLSALSNAKEEATKLGTVIGIDLG
                     TTYSCVGVYKNGHVEIIANDQGNRITPSWVAFTDNERLIGEAAKNLAAVNPERTIFDV
                     KRLIGRKFEDKEVQRDMKLVPYKIVSKDGKPYIQVKIKDGEVKVFSPEEISAMILTKM
                     KETAEAFLGKTIKDAVVTVPAYFNDAQRQATKDAGVIAGLNVARIINEPTAAAIAYGL
                     DKKGGEKNILVFDLGGGTFDVSILTIDNGVFEVLATNGDTHLGGEDFDQRIMEYFIKL
                     IKKKHGKDISKDNRALGKLRREAERAKRSLSSQHQVRVEIESLFDGTDFSEPLTRARF
                     EELNNDLFRKTMGPVKKAMDDAGLQKNQIDEIVLVGGSTRIPKVQQLLKDYFDGKEPS
                     KGVNPDEAVAYGAAVQGGILSGEGGDETKDILLLDVAPLTLGIETVGGVMTKFIPRNT
                     VIPTKKSQVFTTYQDQQTTVSIQVFEGERSLTKDCRNLGKFDLTGIPPAPRGTPQIEV
                     TFEVDANGILNVKAEDKGTGKAEKITITNDKGRLSQEEIERMVREAEEFAEEDKKVKE
                     KIDARNALETYVYNMKNQINDKDKLADKLESDEKEKIETATKEALEWLDDNQSAEKED
                     YDEKLKEVEAVCNPIITAVYQRSGGAPGGGASEEEDDSHDEL"
BASE COUNT      695 a    413 c    579 g    545 t
ORIGIN      
        1 atatatataa atctctcatc atttccagtt cttcataact gaaaaagata aacagaggga
       61 tcacattgtg ttgtacctgt agcttagtgt attctacgtt gattacgaag gaagaagcta
      121 tggctgcctg ttcgaggcga ggcaattctc tcgtcgtgct agcgatcgtt ttattaggct
      181 gtctttctgc actttccaat gcaaaggagg aggctacaaa acttggtacc gttataggaa
      241 ttgatcttgg gacaacctat tcctgtgttg gggtgtacaa gaatggtcat gtggagatta
      301 tagctaatga ccaaggaaac aggattacac catcatgggt tgcattcact gataacgaga
      361 gattgattgg tgaggctgcc aagaacctgg ctgcggtcaa cccagaaaga acaatctttg
      421 atgttaagag gctgattgga agaaaatttg aagataagga agtacagagg gatatgaagc
      481 tggtaccgta caagattgtc agcaaggatg gaaagcccta catacaagtc aaaatcaagg
      541 atggagaagt taaggtattt agtcctgagg aaatcagcgc catgattttg acaaagatga
      601 aagagacagc cgaagcattc cttgggaaga ctattaagga tgctgtggtc actgttcctg
      661 catatttcaa tgatgcacaa agacaggcga caaaggatgc tggtgttatt gctggattaa
      721 atgttgctag gatcattaat gagccaacag ctgcagccat tgcctacgga ctggataaga
      781 aaggtggtga gaagaacatt cttgtctttg accttggtgg tgggacattc gatgtcagta
      841 ttctgaccat tgacaatggt gtatttgagg ttcttgcaac aaatggtgac acccatcttg
      901 gaggtgagga ctttgatcaa aggattatgg aatacttcat caagttgatc aagaagaagc
      961 atggaaagga catcagcaag gacaacagag ctcttggaaa gctaaggaga gaagctgagc
     1021 gtgccaagag atcccttagt agccagcacc aagtccgggt tgagattgaa tcattatttg
     1081 atggtactga tttctctgaa ccactgaccc gtgctcgctt tgaggagttg aacaatgatc
     1141 tcttcagaaa gacaatggga cctgtgaaga aggcaatgga tgatgctggc ctacaaaaga
     1201 accagattga tgagattgtt cttgttggtg gaagcaccag aattcctaag gtgcaacagc
     1261 tcctcaaaga ctattttgac gggaaggagc ccagcaaggg tgtaaatcct gatgaagcag
     1321 ttgcttatgg tgctgctgtt caaggtggta tcctcagtgg tgagggtgga gatgaaacta
     1381 aagatatcct tctcctggat gtcgctcctc tcacccttgg tattgaaacc gttggtggtg
     1441 tgatgaccaa atttatcccc agaaataccg ttatcccaac caagaagtcc caggtcttca
     1501 ccacttacca agaccagcag accactgtct ccattcaggt ttttgaaggt gaaaggagtc
     1561 ttaccaagga ctgtaggaat ctcgggaagt ttgatcttac aggaattcct ccagctccaa
     1621 ggggaacccc ccaaattgag gtcacattcg aagttgatgc caatggtatc ttgaatgtca
     1681 aagcagaaga caagggtact ggaaaagcag agaagattac cattacaaac gacaagggcc
     1741 gcttaagcca agaagagatt gagcgtatgg ttcgtgaagc agaagagttt gctgaggaag
     1801 acaagaaagt gaaggagaag attgatgccc gtaacgcttt ggaaacctac gtgtacaaca
     1861 tgaagaatca aatcaacgac aaggacaaat tagctgacaa actcgagtct gatgagaagg
     1921 agaagattga gacagcaacg aaagaagccc ttgaatggtt agacgataac cagagcgccg
     1981 agaaagagga ctatgacgag aagctcaaag aggtcgaggc agtttgcaac ccaatcatca
     2041 cagctgttta tcaacgatca ggtggagccc caggtggcgg tgcatcggaa gaagaagatg
     2101 acagccatga tgagctgtaa cgtgttccaa agaatctcca ttgatactgc aaagatgcgg
     2161 aaatgaggat atatagagta gtttgaagtg agggttagtg caagtttgtt cattcgttct
     2221 gaaatctctc cc
//