From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: B.subtilis pyruvate dehydrogenase complex genes, complete cds;
Date: 12 Aug 92 10:00:28 GMT
Distribution: bionet
Checksum: 35647    19

LOCUS       BACPYDHY     9019 bp ds-DNA             BCT       12-AUG-1992
DEFINITION  B.subtilis pyruvate dehydrogenase complex genes, complete cds;
            PAL-related lipoprotein (slp) gene, complete cds lysine
            decarboxylase (cad) gene, partial cds.
ACCESSION   M57435 M31542
KEYWORDS    PAL-related lipoprotein; cad gene;
            dihydrolipoamide acetyltransferase E2 subunit;
            dihydrolipoamide dehydrogenase E3 subunit; lipoprotein;
            lysine decarboxylase; pdhA gene; pdhB gene; pdhC gene; pdhD gene;
            pyruvate decarboxylase E1-alpha subunit;
            pyruvate decarboxylase E1-beta subunit;
            pyruvate dehydrogenase complex; slp gene;
            structural membrane protein.
SOURCE      B.subtilis (strain 168) BRB1 (sacA321 metB5) cell line DNA, clones
            sc2 and sc4.
  ORGANISM  Bacillus subtilis
            Prokaryotae; Firmicutes; Endospore-forming rods and cocci;
            Bacillaceae.
REFERENCE   1  (bases 1 to 9019)
  AUTHORS   Hemila,H.O., Palva,A., Paulin,L., Arvidson,S. and Palva,I.
  TITLE     Secretory S complex of Bacillus subtilis: Sequence analysis and
            identity to pyruvate dehydrogenase
  JOURNAL   J. Bacteriol. 172, 5052-5063 (1990)
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     -35_signal      525..530
     -10_signal      548..553
     CDS             636..1253
                     /label=ORF5
                     /codon_start=1
                     /translation="MALILTGCMGQDPVDSLHHSLEKAAESEKPFQEEQNTLKELEAK
                     ENQLYDSVMKLNMDDYKKIVALSNKALENVSKHEEHLKLENDSMKKSETEFEEAKTSA
                     EHIKEKDIKEKADAAANHMEKRYTSYGTMYKEYKKALELNKKLYMQLKDKDLTRDDLD
                     QQIDKVNASYEKILKYSGEFNEQTEKYNKAREDLYDAAGYRVKKS"
     -35_signal      1548..1553
                     /gene="pdhA"
     -10_signal      1573..1578
                     /gene="pdhA"
     repeat_unit     1598..1611
                     /rpt_type=inverted
                     /gene="pdhA"
                     /note="putative"
     RBS             1655..1666
                     /gene="pdhA"
     CDS             1677..2792
                     /EC_number="1.2.4.1"
                     /product="pyruvate decarboxylase E-1 alpha subunit"
                     /gene="pdhA"
                     /evidence=EXPERIMENTAL
                     /codon_start=1
                     /translation="MAAKTKKAIVDSKKQFDAIKKQFETFQILNEKGEVVNEAAMPDL
                     TDDQLKELMRRMVFTRVLDQRSISLNRQGRLGFYAPTAGQEASQIATHFALEKEDFVL
                     PGYRDVPQLIWHGLPLYQAFLFSRGHFRGNQMPDDVNALSPQIIIGAQYIQTAGVALG
                     LKKRGKKAVAITYTGDGGRSQGDFYEGINFAGAYKAPAIFVVQNNRYAISTPVEKQSA
                     AETIAQKAVAAGIVGVQVDGMDPLAVYAATAEARERAINGEGPTLIETLTFRYGPHTM
                     AGDDPTKYRTKEIENEWEQKDPLVRFRAFLENKGLWSEEEEAKVIEDAKEEIKQAIKK
                     ADAEPKQKVTDLMKIMYEKMPHNLEEQFEIYTQKESK"
     RBS             2778..2787
                     /gene="pdhB"
     CDS             2796..3773
                     /EC_number="1.2.4.1"
                     /product="pyruvate decarboxylase E1-beta subunit"
                     /gene="pdhB"
                     /evidence=EXPERIMENTAL
                     /codon_start=1
                     /translation="MAQMTMIQAITDALRTELKNDENVLVFGEDVGVNGGVFRATEGL
                     QKEFGEDRVFDTPLAESGIGGLALGLGLNGFRPVMEIQFFGFVYEVMDSVSGQMARMR
                     YRSGGRWTSPVTIRSPFGGGVHTPELHADSLEGLVAQQPGIKVVIPSTPYDAKGLLIS
                     AIRDNDPVVFLEHMKLYRSFRQEVPEEEYTIELGKADVKREGTDLSIITYGAMVHESL
                     KAADELEKDGISAEVVDLRTVSPLDIDTIIASVEKTGRAIVVQEAQKQAGIAANVVAE
                     INDRAILSLEAPVLRVAAPDTVFPFSQAESVWLPNHKDVLETARKVLEF"
     -35_signal      3742..3747
                     /gene="pdhC"
     -10_signal      3769..3774
                     /gene="pdhC"
     repeat_unit     3790..3815
                     /rpt_type=inverted
                     /gene="pdhC"
                     /note="putative"
     RBS             3873..3879
                     /gene="pdhC"
     CDS             3888..5216
                     /EC_number="2.3.1.12"
                     /product="dihydrolipoamide acetyltransferase E2 subunit"
                     /gene="pdhC"
                     /evidence=EXPERIMENTAL
                     /codon_start=1
                     /translation="MAFEFKLPDIGEGIHEGEIVKWFVKPNDEVDEDDVLAEVQNDKA
                     VVEIPSPVKGKVLELKVEEGTVATVGQTIITFDAPGYEDLQFKGSDESDDAKTEAQVQ
                     STAEAGQDVAKEEQAQEPAKATGAGQQDQAEVDPNKRVIAMPSVRKYAREKGVDIRKV
                     TGSGNNGRVVKEDIDSFVNGGAQEAAPQETAAPQETAAKPAAAPAPEGEFPETREKMS
                     GIRKAIAKAMVNSKHTAPHVTLMDEVDVTNLVAHRKQFKQVAADQGIKLTYLPYVVKA
                     LTSALKKFPVLNTSIDDKTDEVIQKHYFNIGIAADTEKGLLVPVVKNADRKSVFEISD
                     EINGLATKAREGKLAPAEMKGASCTITNIGSAGGQWFTPVINHPEVAILGIGRIAEKA
                     IVRDGEIVAAPVLALSLSFDHRMIDGATAQNALNHIKRLLNDPQLILMEA"
     -35_signal      5134..5139
                     /gene="pdhD"
     -10_signal      5160..5165
                     /gene="pdhD"
     RBS             5204..5213
                     /gene="pdhD"
     CDS             5221..6633
                     /EC_number="1.8.1.4"
                     /product="dihydrolipoamide dehydrogenase E3 subunit"
                     /gene="pdhD"
                     /evidence=EXPERIMENTAL
                     /codon_start=1
                     /translation="MVVGDFPIETDTLVIGAGPGGYVAAIRAAQLGQKVTVVEKATLG
                     GVCLNVGCIPSKALINAGHRYENAKHSDDMGITAENVTVDFTKVQEWKASVVNKLTGG
                     VAGLLKGNKVDVVKGEAYFVDSNSVRVMDENSAQTYTFKNAIIATGSRPIELPNFKYS
                     ERVLNSTGALALKEIPKKLVVIGGGYIGTELGTAYANFGTELVILEGGDEILPGFEKQ
                     MSSLVTRRLKKKGNVEIHTNAMAKGVEERPDGVTVTFEVKGEEKTVDADYVLITVGRR
                     PNTDELGLEQVGIEMTDRGIVKTDKQCRTNVPNIYAIGDIIEGPPLAHKASYEGKIAA
                     EAIAGEPAEIDYLGIPAVVFSEPELASVGYTEAQAKEEGLDIVAAKFPFAANGRALSL
                     NETDGFMKLITRKEDGLVIGAQIAGASASDMISELSLAIEGGMTAEDIAMTIHAHPTL
                     GEITMEAAEVAIGSPIHIVK"
     CDS             complement(6678..7052)
                     /product="PAL-related lipoprotein"
                     /gene="slp"
                     /codon_start=1
                     /translation="MRYRAVFPMLIIVFALSGCTLSTINPMKKSRIDNIHHTQILFFS
                     DENQIDQEAPYYDALLDLEKDYPEQIDKMKVYDKKEGWEDEIETVPTLMVVDQRHVVV
                     KIEGCVKKKEDIIKPLQHVLSK"
     RBS             complement(7055..7064)
                     /gene="slp"
     -10_signal      complement(7538..7544)
                     /gene="slp"
     -35_signal      complement(7563..7568)
                     /gene="slp"
     CDS             complement(7633..9018)
                     /product="lysine decarboxylase"
                     /gene="cad"
                     /codon_start=1
                     /translation="KKGAGMDPEFRQFIGENALSIDLINIEPLDDLHAPKGIIKQAQD
                     LAAEAFGADHTFFSVQGTSGAIMTMVMAVCGPGDKIIIPRNVHKSIMTAIVFSGAVPI
                     FIHPEIDNELGISHGITLESAKRALTEHPDAKGLLVINPTYFGVAADLKSIVELAHSF
                     DVPVLVDEAHGVHIHFHDELPLSAMQAGADIAATSVHKLGGSLTQSSILNMREGLVSK
                     DRVQSILSMLTTTSTSYLLLASLDVARKRLATEGRQLAEETLKLANQTRDRLNQIEGI
                     YCVGSEILGSKAAYSYDPTKLIISVKSLGLTGHDVEKWLRESFNIEVELSDLYNILCI
                     FTPGDSQNDADRLVEALTEIAQQMSEQDVTHQQTEVLLPEIPLLAMTPRDAFYANTEV
                     IPLKEASGRIIAEFVMVYPPGIPIFIPGEIITEENISYIFKNLDAGLPVQGPEDSTLH
                     MIRVIKEQKAIQ"
                     /partial
BASE COUNT     2796 a   1795 c   1982 g   2446 t
ORIGIN
        1 gctgctgttt ttctgcatca gttggaggca gctcaacagg ttcggcagtc tctctgaggg
       61 ctggatggcc gtcacgtacg atgttttcca tagtaatcaa gaaacttcac tcctaaagct
      121 taatatgtaa attatatcgg atttcttcac aaaggtgcaa cgaaacgaat gaccagccgt
      181 atatggctgg tcaatttcat gctgtgtggt tagcaacagc ctccacctaa ataagcagct
      241 ccgacaatga ttaacagaat gaataaaaca acgattagcg caaaggtgcg gccgtagcca
      301 tatccgcagc caccatatcc gtaaccacca taactgcagc agcctccgcc gtaaccatat
      361 ccatacatat tacataacct cctcttttaa ttacgccaga gcctttataa gtaggcatag
      421 cgtctaatat aacctatgta aaaacaaaaa agttgtatgg tcatctgtcc tgatttttca
      481 cttttttgat ttaatttcag tattgcattt atacagcacg gctattgcca aatgtcccca
      541 tggattctat agtagaaatt gtcaagattg ggggatttaa cgtgaaagac gctataaaac
      601 ggcggcctgg aatcaagatc ggagcttttt gtgccatggc actgatatta acaggatgca
      661 tgggacagga cccggttgat tcacttcatc attcattaga aaaagccgcg gaatcggaaa
      721 agccgtttca agaagaacaa aacacactca aagaattaga ggccaaagaa aatcagcttt
      781 acgatagtgt tatgaagctc aatatggatg attataaaaa aattgttgct ttatctaata
      841 aggcgcttga aaatgtcagc aagcacgaag aacacctaaa gcttgaaaat gacagcatga
      901 aaaaatcgga aacagaattt gaggaagcaa aaacgtcagc ggaacatatt aaggaaaaag
      961 acataaaaga aaaggccgat gccgcagcaa accatatgga gaaaagatat acatcatatg
     1021 gcactatgta taaagagtat aaaaaagcgc ttgagcttaa taaaaaactg tatatgcagc
     1081 tgaaagacaa agatttgaca agagatgatc tggatcagca gatcgataaa gtcaatgctt
     1141 cttatgaaaa gatccttaaa tatagcggag aatttaatga acaaaccgaa aaatataata
     1201 aagcccggga agatttatat gatgctgcag gctatcgtgt gaaaaagagc tgactcgatc
     1261 agctcttttt ttgaatgaaa tgcgattcgg atttcgttca aaattttcat ttctattgta
     1321 gtacagcata gtaaggtata ctagtacagt tttgggtgcg gttggcatct gtttttttga
     1381 tgaaaatgta aagcgaatac atttattgaa aatcagcgat gaaaacgatt gacggttctt
     1441 ctttatttgg tgtaaactaa tccatgttaa gcggtgttgt attagttttt tacaacttcg
     1501 aaataataca gttcaaagca gagtgtaact atcctacata aaaggttttg gcaaactggg
     1561 gatcggctaa aatataatac gacttactgc tgatacttta gggtacccta agtttgtatg
     1621 tataaaagga atgcacgtct aatgactgtt taaaagaaag gaagaggtga cttagtatgg
     1681 ctgcaaaaac gaaaaaagct atcgttgaca gtaagaagca atttgatgcc attaaaaagc
     1741 agttcgaaac gttccaaatt ttaaatgaaa agggagaagt cgtgaatgaa gcggcgatgc
     1801 ctgatttaac tgatgatcaa ttaaaagagc taatgcgccg catggtattt acgcgtgtgc
     1861 ttgaccaacg ctctatctca ttaaaccgtc aaggacgtct cggattttac gctcctactg
     1921 cgggtcaaga agcttctcag attgcaacgc atttcgcgct tgaaaaagaa gactttgttc
     1981 ttcctggata ccgtgatgtg cctcagttaa tttggcacgg ccttccatta tatcaagcgt
     2041 ttcttttctc tcgcggacat ttcagaggaa accaaatgcc tgatgatgtg aatgcccttt
     2101 ctccacaaat cattatcggt gctcaataca ttcaaactgc cggtgttgcg ctaggtctta
     2161 aaaaacgcgg taagaaagct gtcgcaatca cttacactgg tgacggcgga cgttctcaag
     2221 gggacttcta cgaaggaatt aactttgccg gagcttataa agcacctgca atcttcgtgg
     2281 tgcaaaacaa ccgttacgcg atttcaactc ctgttgaaaa acaatctgca gctgaaacaa
     2341 ttgcacaaaa agctgtagct gccggtattg tcggtgtaca agtagacgga atggatccgc
     2401 ttgctgtata cgcagcaact gctgaagcac gcgagcgcgc aatcaacggc gaaggtccaa
     2461 cactaattga aacacttaca ttccgttatg gcccgcacac aatggctggt gacgatccta
     2521 ctaaatatcg tacaaaagaa atcgaaaatg agtgggaaca aaaagatccg cttgtacgtt
     2581 tccgtgcgtt ccttgaaaac aaaggcttat ggtctgaaga agaagaagca aaagtgattg
     2641 aggatgcgaa agaagaaatt aagcaagcga tcaaaaaagc tgatgctgaa ccgaagcaaa
     2701 aagtaactga tttaatgaaa atcatgtacg aaaaaatgcc tcacaacctt gaggagcaat
     2761 ttgaaattta tacacagaag gagtcgaagt aagccatggc gcaaatgaca atgattcaag
     2821 caatcacgga tgcgttacgc acagaactga aaaatgacga aaatgtctta gttttcggag
     2881 aagacgttgg tgtaaacggc ggcgtattcc gtgcgacaga aggattgcaa aaagaattcg
     2941 gtgaagaccg tgtgtttgac acgccacttg ctgaatctgg tatcggcggt cttgcccttg
     3001 gtttaggctt aaacggcttc cgtccggtaa tggaaatcca attcttcgga tttgtttatg
     3061 aagtaatgga ttcagtttct ggccaaatgg ctcgtatgcg ctaccgttct ggcggacgct
     3121 ggacttcacc tgtaacaatt cgttctccat tcggcggcgg tgttcatact cctgaacttc
     3181 acgctgacag cttggaaggt cttgttgcac aacagcctgg tatcaaagtt gttattccat
     3241 caactcctta cgatgccaaa ggacttttaa tttctgcgat cagagacaat gatcctgttg
     3301 tcttcttaga gcatatgaag ctttaccgtt ctttccgtca ggaagttcct gaagaagaat
     3361 acacaattga gcttggaaaa gctgacgtga aacgtgaagg tactgatctt tcaatcatca
     3421 cttacggcgc aatggttcat gaatcattaa aagctgctga tgagcttgaa aaagacggca
     3481 tttctgctga agttgtcgac cttcgtacag taagcccact tgatatcgat acaattatcg
     3541 cctctgtaga aaaaacagga cgcgcgattg tcgttcaaga ggcacaaaaa caagccggta
     3601 ttgctgcaaa cgtagtagca gaaattaatg accgtgcgat cctgagcttg gaagcacctg
     3661 tacttcgcgt tgcagcgcca gatacagtat ttcctttctc tcaagcggag agcgtatggc
     3721 ttccaaacca taaagacgtt cttgaaacag caagaaaagt gcttgaattt taatcaaact
     3781 gcataatcga gagggaagat gaacgttttc cctctattat atatctgttt tcaatgctta
     3841 cgatgtaaac tttaaaattg cttaatcaaa ctaggaggtc gagaactgtg gcatttgaat
     3901 ttaaacttcc agatatcggg gaaggtatcc acgaaggcga aatcgtaaaa tggtttgtca
     3961 agcctaacga cgaggtagac gaagatgatg tactggctga agtccaaaat gataaagcag
     4021 tagtagaaat tccttcacct gttaaaggaa aagtattaga attaaaagtt gaagagggaa
     4081 cggttgcaac tgttggacaa acgattatta cgtttgatgc acctggttac gaagatcttc
     4141 aatttaaagg cagcgacgag tctgacgatg cgaaaactga agcacaagtt cagtcaactg
     4201 cagaagctgg acaagacgtt gcgaaagaag agcaagctca agagcctgca aaagctactg
     4261 gcgcaggaca gcaggatcaa gctgaagttg acccgaacaa acgcgtgatc gctatgcctt
     4321 ccgtacgtaa atatgcacgt gaaaaaggtg tagacatccg taaggttact ggttcaggca
     4381 acaacggacg tgttgtaaaa gaagatatcg acagctttgt aaacggagga gcgcaagaag
     4441 ctgcaccgca agaaacagct gcaccacaag aaacagctgc taaaccggct gctgcaccag
     4501 ctccagaggg cgaattccca gaaacacgcg aaaaaatgag cggtatccgt aaagcaattg
     4561 caaaagcgat ggttaactct aaacacactg ctcctcacgt aacgttaatg gacgaagtgg
     4621 acgtaacaaa ccttgttgca catcgtaaac agttcaaaca ggttgctgct gatcaaggaa
     4681 tcaagctgac ttacttgcct tacgttgtaa aagctcttac atctgcactg aaaaaattcc
     4741 ctgttttaaa cacgtcaatt gacgataaaa cagatgaagt catccaaaaa cattacttca
     4801 acatcggtat cgctgctgac actgaaaaag gcttgcttgt accggttgtg aaaaatgcag
     4861 atcgtaaatc tgtctttgaa atttctgatg aaatcaatgg ccttgcaaca aaagctcgtg
     4921 aaggcaagct tgctccagct gaaatgaaag gcgcatcttg cacaattaca aacatcggtt
     4981 ctgccggcgg acaatggttc actccggtta tcaaccatcc agaagttgcg attcttggta
     5041 tcggacgcat tgcagaaaaa gcgattgttc gtgatggcga aatcgtagca gctccagtct
     5101 tagctctttc tctcagcttc gaccaccgta tgatcgacgg agcaactgcg caaaatgcat
     5161 taaatcacat caagcgttta ctgaacgatc cacaattaat tttaatggag gcgtaatgtt
     5221 atggtagtag gagatttccc tattgaaaca gatactcttg taattggtgc gggacctggc
     5281 ggctatgtag ctgccatccg cgctgcacag cttggacaaa aagtaacagt cgttgaaaaa
     5341 gcaactcttg gaggcgtttg tctgaacgtt ggatgtatcc cttcaaaagc gctgatcaat
     5401 gcaggtcacc gttatgagaa tgcaaaacat tctgatgaca tgggaatcac tgctgagaat
     5461 gtaacagttg atttcacaaa agttcaagaa tggaaagctt ctgttgtcaa caagcttact
     5521 ggcggtgtag caggtcttct taaaggcaac aaagtagatg ttgtaaaagg tgaagcttac
     5581 tttgtagaca gcaattcagt tcgtgttatg gatgagaact ctgctcaaac atacacgttt
     5641 aaaaacgcaa tcattgctac tggttctcgt cctatcgaat tgccaaactt caaatatagt
     5701 gagcgtgtcc tgaattcaac tggcgctttg gctcttaaag aaattcctaa aaagctcgtt
     5761 gttatcggcg gcggatacat cggaactgaa cttggaactg cgtatgctaa cttcggtact
     5821 gaacttgtta ttcttgaagg cggagatgaa attcttcctg gcttcgaaaa acaaatgagt
     5881 tctctcgtta cacgcagact gaagaaaaaa ggcaacgttg aaatccatac aaacgcgatg
     5941 gctaaaggcg ttgaagaaag accagacggc gtaacagtta ctttcgaagt aaaaggcgaa
     6001 gaaaaaactg ttgatgctga ttacgtattg attacagtag gacgccgtcc aaacactgat
     6061 gagcttggtc ttgagcaagt cggtatcgaa atgacggacc gcggtatcgt gaaaactgac
     6121 aaacagtgcc gcacaaacgt acctaacatt tatgcaatcg gtgatatcat cgaaggaccg
     6181 ccgcttgctc ataaagcatc ttacgaaggt aaaatcgctg cagaagctat cgctggagag
     6241 cctgcagaaa tcgattacct tggtattcct gcggttgttt tctctgagcc tgaacttgca
     6301 tcagttggtt acactgaagc acaggcgaaa gaagaaggtc ttgacattgt tgctgctaaa
     6361 ttcccatttg cagcaaacgg ccgcgcgctt tctcttaacg aaacagacgg cttcatgaag
     6421 ctgatcactc gtaaagagga cggtcttgtg atcggtgcgc aaatcgccgg agcaagtgct
     6481 tctgatatga tttctgaatt aagcttagcg attgaaggcg gcatgactgc tgaagatatc
     6541 gcaatgacaa ttcacgctca cccaacattg ggcgaaatca caatggaagc tgctgaagtg
     6601 gcaatcggaa gtccgattca catcgtaaaa taattttcat atcaaaaaca gccccgcttt
     6661 gagcgagggc tgttttttta ttttgacagc acatgctgta atggtttaat gatgtcttct
     6721 ttctttttca cacatccctc gattttcacc acgacatggc gctgatcaac aaccataagg
     6781 gtagggacag tttcaatctc atcttcccag ccttcttttt tatcatagac cttcatttta
     6841 tctatttgct cgggataatc tttttctaaa tcaagcagtg catcgtaata aggcgcttcc
     6901 tggtctattt ggttctcatc agaaaaaaac aggatttgtg tatgatgaat gttgtcaatc
     6961 cggctttttt tcatcggatt gatggtggaa agcgtacagc ccgataaagc aaatactatt
     7021 atcagcatgg ggaataccgc gcgatatcgc atctatcttc tcctctgtat aaacagtagt
     7081 taactagctt tagtttatca cgagagacgg cttaatctgc ttcttgttac ctaattgtta
     7141 tataattgaa accgttttta aagtgagatt acatattata tcaatcgttt aaaatagcaa
     7201 cctggggtat attgaatttt ttgctgcatt cttggatgaa agttcaagaa aacgtgcaat
     7261 tacttatgtg ctgtggcata tatatcgagt aaagcttctg tatgaagaag atattgtatg
     7321 atggaaaatg gaaaaacaag gtgaatatga tgatatgaaa taaaatgtgt tatacagttt
     7381 gttgttgaca ttttaaatgt gacatattaa tataataaca acaaaagaaa ttaagcgttt
     7441 tcaaatttca aagaaatggg gaataagaga tgggtacgat tgtttgccaa gattgcaacg
     7501 aagccattca ttactttgaa gatgagaaag tgacaacatt atatggaact tgctgcggac
     7561 aatgtcaatg tcctgttgat gaagagtaaa aaaaagcatg cggcttaaag ccgcatggct
     7621 ttttattttt tattgaattg ctttttgttc tttgatgacc cggatcatat gtaaagtcga
     7681 gtcctcaggt ccttgaacag ggaggcctgc gtcaaggttt ttaaaaatgt agctgatgtt
     7741 ttcttctgta ataatttctc ctgggatgaa gattggaata ccaggcggat ataccatgac
     7801 aaattcagca ataatccgtc cggatgcttc ttttaatggg atgacttccg tatttgcata
     7861 gaaagcatca cgcggagtca ttgccaataa aggtatttct ggaagcagaa cctcagtttg
     7921 ctgatgtgtt acatcttgtt ctgacatttg ctgtgcgatc tcagttaaag cctctacaag
     7981 ccggtctgca tcattttggc tgtcaccagg agtgaaaata cacaaaatat tgtacagatc
     8041 agaaagctcc acctcaatat taaaggattc acgaagccat ttttccacgt catgtcctgt
     8101 caggccaaga cttttcacag agataatcaa ttttgtcgga tcatagctgt aagctgcttt
     8161 cgacccaaga atctcagaac cgacacaata aatgccttca atctgattga ggcgatctct
     8221 cgtctgattg gcaagcttga gtgtttcctc agcaagctgg cggccttctg ttgcaaggcg
     8281 ttttctggca acatccaaag aagcgagaag caagtaagaa gttgacgtag ttgtcagcat
     8341 gctcaggatg gattgcactc tgtctttaga tacaaggcct tctctcatgt ttaaaataga
     8401 gctttgtgtc agtgatccgc ccagcttgtg tacacttgtc gctgctatgt ccgctcctgc
     8461 ctgcatagcc gatagcggca attcgtcgtg aaaatgaatg tgaacgccgt gtgcttcatc
     8521 cacaagcact ggcacatcaa acgaatgagc gagttctacg atgcttttta agtcggctgc
     8581 aacaccgaaa tatgtcgggt tgatgacgag aagccctttt gcatccggat gctcggtaag
     8641 cgcccgttta gcagattcaa gtgtaattcc gtgggagatg cccaattcat tgtcaatttc
     8701 aggatgaata aaaattggaa ccgctccgga aaatacaata gctgtcataa ttgatttatg
     8761 aacatttctt ggaataataa ttttatcacc aggtccgcaa acagccatta ccattgtcat
     8821 aatggcgccg cttgttcctt gaacagaaaa aaatgtatga tccgctccaa atgcttcagc
     8881 agccagatct tgtgcttgtt taataatgcc ttttggcgcg tgcagatcgt ctaatggttc
     8941 aatattaatc aaatctatgc ttaacgcgtt ttcaccgata aattgtctga attctgggtc
     9001 cattcctgcc cctttttta
//