From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Pseudomonas aeruginosa genes for tRNA-Ser and 24K membrane protein.
Date: 29 Dec 92 21:38:57 GMT
Distribution: bionet
Checksum: 11751 5

LOCUS       PSEATTB      2020 bp ds-DNA             BCT       15-DEC-1992
DEFINITION  Pseudomonas aeruginosa genes for tRNA-Ser and 24K membrane protein.
ACCESSION   D13407
KEYWORDS    24K membrane protein; serine tRNA.
SOURCE      Pseudomonas aeruginosa (strain PA158,58-F,PA01) DNA.
  ORGANISM  Pseudomonas aeruginosa
            Prokaryotae; Gracilicutes; Scotobacteria; Pseudomonadaceae.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Hayashi,T., Matsumoto,H., Ohnishi,M. and Terawaki,Y.
  TITLE     Molecular analysis of a cytotoxin-converting phiCTX of Pseudomonas
            aeruginosa: structure of attP-cos-ctx region and integration into
            the serine tRNA gene
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
COMMENT     Submitted (15-Oct-1992) to DDBJ by: Tetsuya Hayashi Department of
            Bacteriology Shinshu University School of Medicine 3-1-1 Asahi
            Matsumoto 390 Japan Phone:  0263-35-4600 x5202 Fax:
            0263-33-6458.
FEATURES             Location/Qualifiers
     misc_feature    516..545
                     /note="This feature applies to a gene which lacks a coding
                     region feature. "
                     /gene="attB"
     terminator      complement(481..505)
     misc_feature    516..545
                     /gene="attB"
                     /note="core sequence of attB"
     tRNA            complement(519..607)
                     /product="tRNA-Ser"
     misc_feature    complement(615..621)
                     /note="stringency control element"
     promoter        complement(623..652)
     promoter        662..689
     RBS             721..727
     terminator      1432..1450
     CDS             738..1406
                     /product="24K membrane protein"
                     /codon_start=1
                     /translation="MQEQQYQLNSAVAEQREVSGVLRNTYGLLALTLAFSGLVAYVSQ
                     QMRLPYPNVFVVLIGFYGLFFLTVKLRNSAWGLVSTFALTGFMGYTLGPILNMYLGLP
                     NGGSVITSAFAMTALVFFGLSAYVLTTRKDMSFLSGFITAGFFVLLGAVLVSLFFQIS
                     GLQLAISAGFVLFSSAMILYQTSAIIHGGERNYIMATISLYVSIYNLFISLLQIFGIA
                     GGDD"
BASE COUNT      361 a    700 c    592 g    367 t
ORIGIN      43-45 min. on PA01 chromosome.
        1 actatgcgaa acgccacatc cccggcgccg cctgggtatt gcgcagccag ttgaagcagg
       61 cactggaacc ggctgggcac ggccgagcgc tacgtactca cctgcggcag cagcctgctg
      121 gcgcgcttcg ccgtggcgga agtccaggca ctcagcggca agccggtgtt ccttctcgac
      181 ggcggcacca gcgcctgggt cgccgcgggc ctgccaacgg aagacggcga aagcctgttg
      241 gcgtcgccgc gcatcgaccg ctaccgccgc ccctacgaag gtaccgacaa tccgcgcgaa
      301 gcgatgcagg gctacctgga ctgggagttc ggcctggtgg aacaactcgg tcgcgacgga
      361 acccacggct tcttcgtgat ctgaagccat tacccgacga aatcggagag tcatcgaatc
      421 gacgccgggc aggctgcgga aacgacccgg aagtgcggaa gagcggcgcc ccgaaaatgc
      481 aaaagccccg ctcagcgggg cttttcagta cgcggatatg gcggaggcgg tgagattcga
      541 actcacggaa gagtctcccc ttcgacggtt ttcaagaccg ttgccttaaa ccactcggcc
      601 acacctccgc atcgcggggc gccatagtac cggatagcaa cacactgtca aactctatcg
      661 attggccctg gcagaggctt tgctatcatg cccatcagta cgtcatggac ccgttcccgt
      721 taaggagtac ccacaccatg caagaacagc aatatcagct gaactccgcc gtcgcggaac
      781 agcgtgaagt cagcggcgtt ctgcgcaata cctacggcct gctggcactc accctggcct
      841 tcagcggcct ggtggcctac gtttcgcagc agatgcgcct gccctatccg aacgtgttcg
      901 tggtgctgat cggcttctac ggcctgttct tcctcaccgt gaagctgcgc aacagcgcct
      961 ggggtctggt cagcactttc gccctgaccg gcttcatggg ctacacgctc ggcccgatcc
     1021 tcaacatgta cctcggcctg cccaacggcg gcagcgtcat cacttcggcg ttcgccatga
     1081 ccgccctggt gttcttcggc ctgtccgcct atgtgctgac cacccgcaag gacatgagct
     1141 tcctgtccgg cttcatcacc gccggcttct tcgtcctgct gggcgccgtg ctggtatcgc
     1201 tgttcttcca gatcagtggc ctgcaactgg cgatcagcgc cggcttcgtc ctgttctcct
     1261 cggcgatgat cctctaccag accagcgcga tcatccacgg cggcgaacgc aactacatca
     1321 tggccaccat cagcctgtac gtgtcgatct acaacctgtt catcagcctg ttgcagatct
     1381 tcggcatcgc cggcggcgac gactgataga aaacgcctcc ggaaaagccc gtccccgcga
     1441 cgggcttttc atttgcggac cggcaaaaca caggcattgg tttatcattc gaataaatcc
     1501 ttcgcgtatc gcagcatgaa attcgccatc gccctattcg accctcccca ctcccccgcc
     1561 gcccgccgcg ctgcgcttca gcgaagccgc gctggccggc gggcacgaaa tcgtccgcct
     1621 gttcttctac caggacggcg tgcacagcgc ctcggccaac gtcgtgagcg gccaggacga
     1681 gttcgacctg cccgccgcct ggcgcgagtt ggtcgagcgc aacggcctgg acgccgtggt
     1741 ctgcatcgcc gccgccctgc gccgcggcgt gctgaatgcc gaggaagccg aacgctacgg
     1801 ccgtcccggc gccaaccttg gcgcaccatg ggaactgtcc ggactcggcc agttgcacga
     1861 ggcggcgcaa agcgcggacc gcctggtctg cttcggaggc gaccgatgag ccagtcgctg
     1921 ctgatcatca gccgccagtc gccctggtcc ggcccgagcg cccgcgaggc gctggacata
     1981 gccctggccg gcggcgcctt cgatctaccg gtcggcatgc
//