From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: E.coli lysP gene for lysine specific permease
Date: 10 Apr 92 10:04:38 GMT
Distribution: bionet
Checksum: 05651     6

LOCUS       ECOLYSPP     2661 bp ds-DNA             BCT       10-APR-1992
DEFINITION  E.coli lysP gene for lysine specific permease
ACCESSION   X65029
KEYWORDS    lysP gene; lysine specific permease.
SOURCE      Escherichia coli DNA.
  ORGANISM  Escherichia coli
            Prokaryota; Bacteria; Gram-negative facultatively anaerobic rods;
            Enterobacteriaceae.
REFERENCE   1  (bases 1 to 2661)
  AUTHORS   Steffes,C., Ellis,J., Wu,J. and Rosen,B.P.
  TITLE     The lys gene encodes the lysine specific permease
  JOURNAL   J. Bacteriol. (1992) In press
  STANDARD  full automatic
COMMENT     *source: clone_library=Kohara lambda; *source: clone=7H12;
            From EMBL    entry ECLYSPP;  dated 20-MAR-1992.
FEATURES             Location/Qualifiers
     RBS             66..69
     RBS             508..513
     CDS             523..1992
                     /product="lysine specific permease"
                     /gene="lysp"
                     /translation="MVSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELARYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRKAAVCTTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     terminator      2049
                     /note="rho-dependent"
     CDS             2286..>2661
                     /gene="cir"
                     /translation="MFRLNPFVRVGLCLSAISCAWPVLAVDDDGETMVVTASSVEQNL
                     KDAPASISVITQEDLQRKPVQNLKDVLKEVPGVQLTNEGDNRKGVSIRGLDSDYTLIL
                     VDGKRVNSRNAVFRHNDFDLNWI"
BASE COUNT      566 a    640 c    724 g    731 t
ORIGIN
        1 ggatcctcgg tgaacgcggt tccggcacgc ggagattgtc gattatctgt tgctgtcaca
       61 tttaccgaag tttgagatgg cgatggaatt aggtaactcc gaggcaatca aacatgcggt
      121 gcgtcatggg ttgggaatta gttgcctgtc gcgacgtgtg attgaagatc aattgcaggc
      181 aggcacatta agtgaagttg cggtccctct gccgcgcctg atgcgtacgt tgtggcgtat
      241 acatcatcgg caaaaacacc tttccaacgc gctacggcgc tttctggact attgcgatcc
      301 cgcaaatgtg cgcgttaagt tgctgtacaa gaacatgctg gtgctgtgtc gatttcgtga
      361 cgcagcgcct tcagcatgca ttcgccagaa aagagattgg ctgctttact tataatccct
      421 gggcgatcat gaaggtgtct tataaccgtg tatttctgcc ggaaggattg ccaatcgtct
      481 gctacaatcg cgcctcattt ttaagatgga tagcattttt gtatggtttc cgaaactaaa
      541 accacagaag cgccgggctt acgccgtgaa ttaaaggcgc gtcacctgac gatgattgcc
      601 attggcggtt ccatcggtac aggtcttttt gttgcctctg gcgcaacgat ttctcaggca
      661 ggtccgggcg gggcattgct ctcgtatatg ctgattggcc tgatggttta cttcctgatg
      721 accagtctcg gtgaactggc tcgatatatg ccggtttccg gttcgtttgc cacttacggt
      781 cagaactatg ttgaagaagg ctttggcttc gcgctgggct ggaactactg gtacaactgg
      841 gcggtgacta tcgccgttga cctggttgca gctcagctgg tcatgagctg gtggttcccg
      901 gatacaccgg gctggatctg gagtgcgttg ttcctcggcg ttatcttcct gctgaactac
      961 atctcagttc gtggctttgg tgaagcggaa tactggttct cactgatcaa agtcacgaca
     1021 gttattgtct ttatcatcgt tggcgtgctg atgattatcg gtatcttcaa aggcgcgcag
     1081 cctgcgggct ggagcaactg gacaatcggc gaagcgccgt ttgctggtgg ttttgcggcg
     1141 atgatcggcg tagctatgat tgtcggcttc tctttccagg gaaccgagct gatcggtatt
     1201 gctgcaggcg agtccgaaga tccggcgaaa aacattccac gcgcggtacg tcaggtgttc
     1261 tggcgaatcc tgttgttcta tgtgttcgcg atcctgatta tcagcctgat tattccgtac
     1321 accgatccga gcctgctgcg taacgatgtt aaagacatca gcgttagtcc gttcaccctg
     1381 gtgttccagc acgcgggtct gctctctgcg gcggcggtga tgaacgcagt tattctgacg
     1441 gcggtgctgt cagcgggtaa ctccggtatg tatgcgtcta ctcgtatgct gtacaccctg
     1501 gcgtgtgacg gtaaagcgcc gcgcattttc gctaaactgt cgcgtggtgg cgtgccgcgt
     1561 aaggccgctg tatgcacgac ggtgattgcc ggtctgtgct tcctgacctc catgtttggc
     1621 aaccagacgg tatacctgtg gctgctgaac acctccggga tgacgggttt tatcgcctgg
     1681 ctggggattg ccattagcca ctatcgcttc cgtcgcggtt acgtattgca gggacacgac
     1741 attaacgatc tgccgtaccg ttcaggtttc ttcccactgg ggccgatctt cgcattcatt
     1801 ctgtgtctga ttatcacttt gggccagaac tacgaagcgt tcctgaaaga tactattgac
     1861 tggggcggcg tagcggcaac gtatattggt atcccgctgt tcctgattat ttggttcggc
     1921 tacaagctga ttaaaggaac tcacttcgta cgctacagcg aaatgaagtt cccgcagaac
     1981 gataagaaat aagtttcctc ccttccttgc taagccctct caaccgagag ggctttttca
     2041 attccatttc cctgacaaat catgcggata taaatttaac atttgggatt gataattgtt
     2101 atcgtttgca ttatcgttac gccgcaatca aaaaaggctg acaaatcaga ggctgttccg
     2161 gctttctggg atgatcacct gcataaaaaa taagtccacc gcgatgctgc cgtacgcaag
     2221 gggacgtgaa gaagatgtga gcgataaccc attttatttt cgtagttacc tcatggagat
     2281 atggaatgtt taggttgaac cctttcgtac gggtcgggct gtgtttgtcc gctatttctt
     2341 gtgcatggcc tgtgttagcg gtcgatgatg atggcgaaac gatggttgtc actgcatctt
     2401 ccgtggaaca aaatcttaaa gatgcacctg ccagtatcag cgtcattacc caggaagacc
     2461 tgcagcgaaa accggtacag aatctgaagg atgtcctcaa agaagtgcct ggcgtacaac
     2521 tgacgaacga aggggataac cgtaagggcg ttagtattcg tggtctggac agcgactata
     2581 ccctgattct cgtcgacggt aaacgcgtga actcccgcaa tgccgtcttc cgccacaatg
     2641 atttcgatct gaactggatc c
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