From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Bacillus subtilis protease, 3' end, dipicolinate synthase subunits
Date: 13 Jan 93 05:45:54 GMT
Distribution: bionet
Checksum: 08016 16

LOCUS       BACSPOVFAB   7016 bp ds-DNA             BCT       12-JAN-1993
DEFINITION  Bacillus subtilis protease, 3' end, dipicolinate synthase subunits
            A and B (spoVFA and spoVFB), aspartate semialdehyde dehydrogenase
            (asd), aspartokinase I (dapG), dihydropdipicolinate synthase (dapA)
            genes, complete cds.
ACCESSION   L08471
KEYWORDS    asd gene; aspartate semialdehyde dehydrogenase; aspartokinase I;
            dapA gene; dapG gene; diaminopimelate-sensitive isozym;
            dihydropdipicolinate synthase; dipicolinate synthase; protease;
            spoVFA gene; spoVFB gene.
SOURCE      Bacillus subtilis (strain 168) DNA.
  ORGANISM  Bacillus subtilis
            Prokaryotae; Firmicutes; Endospore-forming rods and cocci;
            Bacillaceae.
REFERENCE   1  (bases 1 to 7016)
  AUTHORS   Chen,N.-Y., Jiang,S.-q., Klein,D.A. and Paulus,H.
  TITLE     Organization and nucleotide sequence of the Bacillus subtilis
            diaminopimelate operon, a cluster of genes encoding the first three
            enzymes of diaminopimelate synthesis and dipicolinate synthase
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     misc_feature    5653..5659
                     /note="This feature applies to a gene which lacks a coding
                     region feature. "
                     /gene="dapA"
     terminator      1145..1181
     RBS             1206..1215
                     /note="for ORFZ"
     terminator      1522..1552
     promoter        1581..1611
                     /note="'sigma K promoter used during sporulation'"
     prim_transcript 1612..>6595
                     /note="'sporulation transcript, presumably transcribed by
                     sigma K RNApolymerase'"
     RBS             1644..1654
                     /gene="spoVFA"
     RBS             2544..2552
                     /gene="spoVFB"
     promoter        3199..3232
                     /note="'sigma A promoter used during growth'"
     prim_transcript 3233..>6595
                     /note="'vegetative transcript, presumably transcribed by
                     sigma A RNApolymerase during growth'"
     prim_transcript 3267..>6595
                     /note="'secondary sporulation transcript, transcribed by
                     an unknown form ofRNA polymerase'"
     RBS             3274..3281
                     /gene="asd"
     misc_signal     4347..4414
                     /note="'putative translation attenuator'"
     RBS             4408..4411
                     /gene="dapG"
     RBS             5653..5659
                     /gene="dapA"
     terminator      6563..6603
     CDS             <1..1143
                     /note="26% amino acid identity with MAS1 ofSaccharomyces
                     cerevisiae'; putative"
                     /product="processing protease"
                     /codon_start=1
                     /translation="IGTGSRHETPEINGISHFLEHMFFKGTSTKSAREIAESFDRIGG
                     QVNAFTSKEYTCYYAKVLDEHANYALDVLADMFFHSTFDENELKKEKNVVYEEIKMYE
                     DAPDDIVHDLLSKATYGNHSLGYPILGTEETLASFNGDSLRQYMHDYYTPDRVVISVA
                     GNISDSFIKDVEKWFGSYEAKGKATGLEKPEFHTEKLTRKKETEQAHLCLGFKGLEVG
                     HERIYDLIVLNNVLGGSMSSRLLEDVREDKGLAYSVYSYHSSYEDSGMLTIYGGTGAN
                     QLQQLSETIQETLATLKRDGITSKELENSKEQMKGSLMLSLESTNSKMSRNGKNQLLL
                     GKHKTLDEIINELNAVNLERVNGLARQLFTEDYALALISPSGNMPS"
     CDS             1221..1478
                     /note="ORFZ"
                     /codon_start=1
                     /translation="MRLSELSGKEIVDIKRAERLGVLGQTDLEINEQDGQITALLIPT
                     VKWFGLRKQGHDIRVPWHHIQKIGSDMIILDVPEEMPPRQE"
     CDS             1664..2557
                     /gene="spoVFA"
                     /product="dipicolinate synthase subunit A"
                     /codon_start=1
                     /translation="MLTGLKIAVIGGDARQLEIIRKLTEQQADIYLVGFDQLDHGFTG
                     AVKCNIDEIPFQQIDSIILPVSATTGEGVVSTVFSNEEVVLKQDHLDRTPAHCVIFSG
                     ISNAYLENIAAQAKRKLVKLFERDDIAIYNSIPTVEGTIMLAIQHTDYTIHGSQVAVL
                     GLGRTGMTIARTFAALGANVKVGARSSAHLARITEMGLVPFHTDELKEHVKDIDICIN
                     TIPSMILNQTVLSSMTPKTLILDLASRPGGTDFKYAEKQGIKALLAPGLPGIVAPKTA
                     GQILANVLSKLLAEIQAEEGK"
     CDS             2560..3162
                     /gene="spoVFB"
                     /product="dipicolinate synthase subunit B"
                     /codon_start=1
                     /translation="MSSLKGKRIGFGLTGSHCTYEAVFPQIEELVNEGAEVRPVVTFN
                     VKSTNTRFGEGAEWVKKIEDLTGYEAIDSIVKAEPLGPKLPLDCMVIAPLTGNSMSKL
                     ANAMTDSPVLMAAKATIRNNRPVVLGISTNDALGLNGTNLMRLMSTKNIFFIPFGQDD
                     PFKKPNSMVAKMDLLPQTIEKALMHQQLQPILVENYQGND"
     CDS             3288..4328
                     /EC_number="1.2.1.11"
                     /gene="asd"
                     /product="aspartate semialdehyde dehydrogenase"
                     /codon_start=1
                     /translation="MGRGLHVAVVGATGAVGQQMLKTLEDRNFEMDTLTLLSSKRSAG
                     TKVTFKGQELTVQEASPESFEGVNIALFSAGGSVSQALAPEAVKRGAIVIDNTSAFRM
                     DENTPLVVPEVNEADLHEHNGIIANPNCSTIQMVAALEPIRKAYGLNKVIVSTYQAVS
                     GAGNEAVKELYSQTQAILNKEEIEPEIMPVKGDKKHYQIAFNAIPQIDKFQDNGYTFE
                     EMKMINETKKIMHMPDLQVAATCVRLPIQTGHSESVYIEIDRDDATVEDIKNLLKEAP
                     GVTLQDDPSQQLYPMPADAVGKNDVFVGRIRKDLDRANGFHLWVVSDNLLKGAAWNSV
                     QIAESLKKLNLV"
     CDS             4420..5634
                     /EC_number="2.7.2.4"
                     /gene="dapG"
                     /note="diaminopimelate-sensitive isozyme"
                     /product="aspartokinase I"
                     /codon_start=1
                     /translation="MKIIVQKFGGTSVKDDKGRKLALGHIKEAISEGYKVVVVVSAMG
                     RKGDPYATDSLLGLLYGDQSAISPREQDLLLSCGETISSVVFTSMLLDNGVKAAALTG
                     AQAGFLTNDQHTNAKIIEMKPERLFSVLANHDAVVVAGFQGATEKGDTTTIGRGGSDT
                     SAAALGAAVDAEYIDIFTDVEGVMTADPRVVENAKPLPVVTYTEICNLAYQGAKVISP
                     RAVEIAMQAKVPIRVRSTYSNDKGTLVTSHHSSKVGSDVFERLITGIAHVKDVTQFKV
                     PAKIGQYNVQTEVFKAMANAGISVDFFNITPSEIVYTVAGNKTETAQRILMDMGYDPM
                     VTRNCAKVSAVGAGIMGVPGVTSKIVSALSEKEIPILQSADSHTTIWVLVHEADMVPA
                     VNALHEVFELSK"
     CDS             5665..6537
                     /EC_number="4.2.1.52"
                     /gene="dapA gene"
                     /product="dihydropdipicolinate synthase"
                     /codon_start=1
                     /translation="MNFGNVSTAMITPFDNKGNVDFQKLSTLIDYLLKNGTDSLVVAG
                     TTGESPTLSTEEKIALFEYTVKEVNGRVPVIAGTGSNNTKDSIKLTKKAEEAGVDAVM
                     LVTPYYNKPSQEGMYQHFKAIAAETSLPVMLYNVPGRTVASLAPETTIRLAADIPNVV
                     AIKEASGDLEAITKIIAETPEDFYVYSGDDALTLPILSVGGRGVVSVASHIAGTDMQQ
                     MIKNYTNGQTANAALIHQKLLPIMKELFKAPNPAPVKTALQLRGLDVGSVRLPLVPLT
                     EDERLSLSSTISEL"
BASE COUNT     2146 a   1402 c   1691 g   1777 t
ORIGIN      
        1 atcggcacag gttcgagaca tgaaacgccg gagataaacg gaatttctca ctttttagag
       61 cacatgttct ttaaagggac gagcacaaaa tctgcacgcg agatagcaga atcttttgat
      121 cgtattggcg gccaggtcaa tgcgtttact tcaaaggaat atacctgcta ctatgcaaag
      181 gtgcttgacg agcatgcgaa ttacgcgctg gacgtattag cagatatgtt ctttcattca
      241 acgtttgatg aaaacgagct gaaaaaagag aaaaatgtag tatatgaaga aattaaaatg
      301 tatgaagatg cgccggacga cattgtacat gatttattga gcaaagccac ttacggcaat
      361 cattctttag gctacccaat tttaggaacg gaagaaacgc ttgcttcctt taacggtgac
      421 tctctcagac aatacatgca tgattattat acgccggacc gagtggtgat ttcggtagcg
      481 ggcaatatat ctgacagttt tatcaaagat gtagaaaaat ggttcgggtc atacgaggcg
      541 aaaggcaaag cgacaggcct tgagaagcct gagttccaca cggaaaaact gacgagaaaa
      601 aaagaaacag agcaggctca tttgtgcctt ggatttaaag gcttagaggt tggccatgaa
      661 cgtatttatg atttaatcgt cctcaacaat gtgctcggag gcagcatgag cagccggctg
      721 ttggaagatg tgcgtgaaga taaaggactt gcttattctg tttacagcta tcacagctca
      781 tatgaggaca gcggaatgct aacgatttac ggcggaacgg gtgcaaatca gcttcagcag
      841 ctgtcggaaa caattcaaga aaccctggcc acattaaaac gtgacggcat tacctcgaaa
      901 gaactggaaa acagcaaaga gcaaatgaag ggaagcttaa tgctgagctt agaaagcaca
      961 aacagcaaaa tgagccgaaa cggaaaaaat caactgctgc tcggcaaaca taaaacatta
     1021 gatgaaatta tcaatgaatt aaacgccgtg aacttagagc gtgttaatgg ccttgccaga
     1081 caattgttta ccgaagacta tgcattagca cttatcagcc cttccggcaa tatgccgtct
     1141 taaaaaggaa agcctgcccc ataatggagc aggcattttt taatcccttt catcatacat
     1201 agtacaaggg ggtgacagac atgcggctca gtgaattatc gggaaaggaa attgtagaca
     1261 tcaaaagagc tgaacggctc ggagtgctcg gccagaccga tttggaaatc aatgaacagg
     1321 atggacagat tacagcactc ctcattccca cagttaagtg gtttggtttg agaaagcaag
     1381 gtcatgacat tcgtgtccca tggcaccata ttcaaaagat cggttcagat atgattatat
     1441 tagatgtacc tgaggaaatg cctcctcgac aagagtaaat agcccaattg actgtgaagc
     1501 gggctctaaa gaaaacatct gaaacatcgg ctgccggagc cgatgttttt ttatatggaa
     1561 aaagcgcatc ttttatattc accggtattt ccttttgatc ataagatgaa ggggagctta
     1621 acaactagag atccagtata tacaaagaag gtgaacgttt agaatgttaa ccggattgaa
     1681 aattgcagtt atcggcggtg acgcaagaca gctcgaaatt ataagaaagc tcactgaaca
     1741 gcaggctgac atctatcttg tcggttttga ccaattggat cacggtttta ccggggcagt
     1801 aaaatgcaat attgatgaaa ttccttttca gcaaatagac agcatcattc ttccagtatc
     1861 cgcgacaaca ggagaaggtg tcgtatcgac tgtattttcg aatgaagaag ttgtgttaaa
     1921 acaggaccat cttgacagaa cgcctgcaca ttgtgtcatt ttctcaggaa tttctaacgc
     1981 ctatttagaa aacattgcag ctcaggcaaa aagaaaactt gttaagctgt ttgagcggga
     2041 tgacattgcg atatacaact ctattccgac agtagaagga acgatcatgc tggctattca
     2101 gcacacggat tatacgatac acggatcaca ggtggccgtt ctcggtctgg ggcgcaccgg
     2161 gatgacgatt gcccgtacat ttgccgcgct cggggcgaat gtaaaagtgg gggcaagaag
     2221 ttcagcgcat ctggcacgta tcactgaaat ggggctcgtt ccttttcata ccgatgagct
     2281 gaaagagcat gtaaaagata tagatatttg cattaatacc ataccgagta tgattttaaa
     2341 tcaaacggta ctttctagca tgacaccaaa aaccttaata ttggatctgg cctcacgtcc
     2401 cgggggaacg gattttaaat atgccgagaa acaagggatt aaagcacttc ttgctcccgg
     2461 gcttccaggg attgtcgctc ctaaaacagc tgggcaaatc cttgcaaacg tcttgagcaa
     2521 gcttttggct gaaatacaag ctgaggaggg gaaataagga tgtcgtcatt aaaaggaaaa
     2581 agaatcgggt ttgggctgac cgggtcgcat tgcacatatg aagcggtttt cccgcaaatt
     2641 gaggagttgg tcaacgaagg agctgaagtc cgtccggttg tcacatttaa tgtaaaatct
     2701 acaaataccc gatttggaga gggcgcagaa tgggttaaaa aaattgaaga cctgactgga
     2761 tatgaggcca ttgattcgat tgtaaaggca gaacctcttg ggccgaagct gccccttgac
     2821 tgcatggtca ttgcgccttt aacaggcaat tcaatgagca agctggcaaa tgccatgacg
     2881 gacagcccgg tgctgatggc ggcaaaagcg acaatccgga acaatcggcc tgtcgttctg
     2941 ggtatctcga caaatgatgc tcttggttta aacggaacaa atttaatgag gctcatgtca
     3001 acaaaaaata tcttttttat tccattcggg caagatgatc catttaaaaa accgaattca
     3061 atggtagcca aaatggatct gcttccgcaa acgattgaaa aggcactcat gcaccagcag
     3121 cttcagccga ttctagttga gaattatcag ggaaatgact aaatttacag aaaatctttc
     3181 ccaaactaaa aaattcggtc atcacccgca tattctatgg taaaataaaa cgtaaaatca
     3241 tagtcaaatc aattcaatga agctgattgg cggaaggagt tttacagatg ggaagaggtt
     3301 tacacgtagc tgttgtcgga gcgacagggg ctgtgggaca acaaatgctt aaaacacttg
     3361 aagacagaaa ctttgaaatg gacacactta cattgctatc ttcaaaacgc tctgcgggga
     3421 caaaagtcac gtttaaaggc caagagctga ctgttcagga agcttctcct gagagctttg
     3481 aaggggtaaa tattgctttg ttcagcgccg gcggaagcgt atcacaagca ttggcaccag
     3541 aagctgtaaa acgcggcgct attgttatag ataatacgag tgcgttccgc atggatgaaa
     3601 atacaccgct tgttgtgcca gaagtgaatg aggcagactt gcatgaacac aacggcatta
     3661 ttgccaatcc aaactgctct acaatccaaa tggttgcggc acttgaaccg atccgcaaag
     3721 catatggctt aaacaaggtc atcgtatcaa cttaccaggc agtatcagga gcgggtaatg
     3781 aagctgtaaa agagctttac agccaaacac aggcgatttt aaataaagaa gaaatagagc
     3841 ctgagatcat gcctgtaaaa ggtgacaaaa aacactatca aatcgccttc aacgcgattc
     3901 cgcaaatcga taaattccaa gataacggct atacgtttga ggaaatgaaa atgataaatg
     3961 aaacgaaaaa aatcatgcac atgcctgacc ttcaagtagc ggctacatgt gtgagactgc
     4021 caatccaaac tgggcactca gagtccgtct acatcgaaat agaccgtgat gacgctacag
     4081 ttgaagatat taaaaatcta ttgaaagaag ctccgggcgt gacacttcag gatgatccaa
     4141 gccagcagct ttatccgatg cctgctgatg ctgtcggcaa aaacgatgtg tttgtaggcc
     4201 gcatccgcaa agacttggac agagcaaacg gttttcattt atgggttgtt tctgataacc
     4261 tattaaaagg cgcggcatgg aactctgttc aaattgcaga aagcctgaaa aaactaaacc
     4321 tcgtataaaa gagagaattt tctaaagacg aatagaagag agtaaggcgc tatcagcctg
     4381 ctctcttctg ttacgtccga ataatttgga gtgaaaacag tgaagataat tgttcaaaag
     4441 ttcggcggaa catcagtaaa agatgataaa ggccgcaaac tggctttagg gcatattaaa
     4501 gaagcaatca gtgaaggata taaggttgtc gttgtcgttt cagcgatggg ccgaaaaggg
     4561 gacccgtatg caacagattc tttgctcggg ctgctttacg gcgatcagtc agcgatttct
     4621 cctagggagc aggatttgct tttatcttgc ggagagacta tttcctctgt cgtctttaca
     4681 agcatgctgc ttgataacgg tgtgaaagca gctgcgctga caggggctca ggctggcttt
     4741 ttaacgaatg atcagcatac taatgcgaaa atcattgaaa tgaaaccgga gcgtttattc
     4801 agtgtgctcg ccaatcatga tgcggtagtc gtcgcaggct tccagggcgc aactgaaaag
     4861 ggtgatacaa caacaatcgg cagagggggc agcgatacgt ctgcagccgc actcggagca
     4921 gctgttgatg cggaatacat cgatattttc accgatgtag aaggcgtgat gactgctgac
     4981 ccaagagtgg tagaaaatgc aaaaccgctt cctgttgtga cgtatacgga aatctgcaac
     5041 ctggcgtatc agggggccaa ggtcatttct ccgcgtgctg tggaaatcgc aatgcaggcc
     5101 aaggttccga tccgcgtccg ttcgacttat tcgaatgata aaggcacatt ggtgacatca
     5161 catcactctt ccaaggtcgg cagtgatgtt tttgaaaggc tgattacggg aatcgcccat
     5221 gtcaaagatg tcacgcagtt caaagtgcct gcgaaaatcg gccagtataa cgttcagact
     5281 gaagtgttca aggctatggc aaacgctgga atcagtgtcg atttctttaa cattacacct
     5341 agtgaaatcg tctatacggt agccggaaat aaaacagaaa cagctcagcg tattttaatg
     5401 gatatgggct atgatccaat ggtgacgaga aactgcgcga aggtatcggc ggtcggcgcg
     5461 ggcattatgg gtgttcccgg tgttacgtcc aaaattgtat cggctctttc agaaaaagaa
     5521 attccgatcc ttcagtcggc tgacagccat acgacgatat gggtgctcgt ccatgaagcg
     5581 gatatggttc ctgctgtaaa cgctttgcat gaagtgtttg aactttcgaa gtaaatgtga
     5641 ttgaatcatg atgaggtgaa taaaatgaat ttcggaaatg tgtctacagc gatgattaca
     5701 ccctttgaca ataaagggaa cgtagacttt caaaaactgt ctacactgat tgattacttg
     5761 ttgaaaaacg gaacggattc tttagtagta gcgggaacaa ctggagaatc tccgaccctt
     5821 tcaactgaag aaaaaattgc gctttttgaa tatacggtaa aagaagtaaa cggacgggtg
     5881 cccgttatcg ctggtactgg gagcaacaac acgaaagatt ccattaagct gacaaaaaaa
     5941 gctgaggaag cgggcgtgga cgctgttatg cttgttaccc cgtattacaa taaaccttct
     6001 caagaaggaa tgtaccagca ttttaaagca attgcggcag agacatctct tccggttatg
     6061 ctctataatg ttcctggccg tacggttgct tctcttgctc ctgaaacgac gatccgtttg
     6121 gcggcagaca ttccgaatgt ggttgcgatt aaagaagcga gcggagacct cgaagcgatt
     6181 acaaaaatta ttgctgaaac acctgaagac ttctatgtct attcagggga tgatgctcta
     6241 acgcttccaa ttctttcagt tggaggtaga ggagttgtgt cagtggcgag ccatattgca
     6301 ggcactgata tgcagcaaat gatcaaaaat tatacgaatg ggcaaacagc taatgcggca
     6361 ctgattcatc agaaactgct gccgattatg aaggaactgt ttaaagcgcc taatcctgct
     6421 ccagtcaaaa cagcgcttca gctgagaggt cttgatgtcg gttctgtgcg tctgcctcta
     6481 gtcccattaa ctgaggatga acgtctgagc ttaagcagca cgatcagcga actgtaagaa
     6541 aatttcatac agtgaaacaa acgcggtcat tctcacattc agctgagttt gaccgtttct
     6601 tttacatatt ggttttccct aaaccttctg ctttatcttg ttttctaaat ttactttcag
     6661 ttataatagg aacaagtgat gatggacggg tatgagacta ggaggatata gattttgaaa
     6721 aagaaaaata cagaaaacgt tagaattatc gcccttggcg gtgtcggaga gatcgggaat
     6781 taccattttt ggacagctgt cttttcagca gcagccatta tgctcggggg ctttctcgct
     6841 gactttttca cagtgcaaat gattttttat gcaagctcta ttctatattt tttgagtgga
     6901 ttgatgatga tgaaaacagg ctgacgcggt cagcctgttt ttttatgcgg ctgaaatgcg
     6961 gcaccgcatc aacgtttttg tgctggagtc gtgctgattt tttttgagcg gaattc
//