From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Bacillus firmus nhaC gene, complete cds.
Date: 15 Sep 92 10:02:21 GMT
Distribution: bionet
Checksum: 42058     5

LOCUS       BACNHAC      1946 bp ds-DNA             BCT       15-SEP-1992
DEFINITION  Bacillus firmus nhaC gene, complete cds.
ACCESSION   M73530
KEYWORDS    .
SOURCE      Bacillus firmus (strain OF4) DNA.
  ORGANISM  Bacillus firmus
            Prokaryotae; Firmicutes; Endospore-forming rods and cocci;
            Bacillaceae.
REFERENCE   1  (bases 1 to 1946)
  AUTHORS   Ivey,D.M., Guffanti,A.A., Bossewitch,J.S., Padan,E. and
            Krulwich,T.A.
  TITLE     Molecular cloning and sequencing of a gene from alkapiphilic
            Bacillus firmus OF4 that functionally complements and Escherichia
            coli strain carrying a deletion in the nhaA Na+/H+ antiporter gene
  JOURNAL   J. Biol. Chem. 266, 23483-23489 (1991)
  STANDARD  simple staff_entry
FEATURES             Location/Qualifiers
     RBS             125..130
     CDS             135..1343
                     /function="pH homeostasis, sodium extrusion"
                     /product="Na+/H+ antiporter"
                     /gene="nhaC"
                     /evidence=EXPERIMENTAL
                     /codon_start=1
                     /translation="MIDGLKIGIKPIFILALVGIVIAVWMMSGTVPTLLFYGLSIISP
                     EWFAVSTLLICMIVSSFTGSSFTTVGTIGVAMMGIGTALGIDPAIAAGAVVCGACFGD
                     KMSPLSDTTNFAPGIVGVDLFDHIRHLLWTTVPSFVITVILFLMIGRSQATSTTQDIQ
                     AMLTALDSQFTLSVLTLLSPLLVVILAMRRFPTIPVLVVGILTGIITAAFVQGNADVV
                     RWFTIIQNGFSMETGNEVIDGIVNRGGLQSMMWSISLVMIALTLGGVIQRIGVIDTLL
                     QSANKKIIFTWTFDCSDSTSSIGVNVVTGEQYLSILLPGKTFESFYTKLNVAKKNLSR
                     TLEDAGTLVNPLIPWGVSGAFFASTLGVDVIDYLPFAFFLFLSPFMTILFGYLGIGVG
                     KEQSLANKRG"
                     /citation=[1]
BASE COUNT      508 a    340 c    442 g    656 t
ORIGIN
        1 tcatgatggc cattattatt gcgagtatgt ttgtgttaaa ggttgaaccg catattccgc
       61 tagttgtatg tcttcttatt gcggctgttt ttgggcgcta tttaagagtt ggttggaatg
      121 atctagagag tgcgatgatt gatggattga aaatagggat caagcctatt tttattttgg
      181 cacttgttgg gattgtcatt gctgtatgga tgatgagcgg aaccgttcct acccttttat
      241 tttatgggtt atccatcatt tcgcctgagt ggtttgctgt tagtacttta ttaatttgca
      301 tgattgtgtc aagctttaca ggaagctcat ttacgacagt tggtacgatt ggagtggcca
      361 tgatgggaat tggtaccgca cttggtattg atcctgctat tgcagctggt gctgtcgttt
      421 gcggggcttg ttttggcgat aaaatgtctc cattatctga tactacaaac tttgctccag
      481 gtattgtcgg agtagactta tttgatcata ttcgccactt gctttggaca accgttccta
      541 gcttcgtcat tacggtcatt ctttttctca tgatagggag aagtcaagca acgagcacga
      601 cacaagatat ccaagcaatg cttacagcgc tcgattctca atttaccctt agcgtcctca
      661 cgcttttatc acctcttcta gttgtgattt tagcaatgag acgttttccg acaatacctg
      721 tattagtagt cgggatattg actggtatta taacagctgc ttttgtccaa gggaatgctg
      781 atgttgttag atggtttact attatccaaa atggtttctc gatggaaaca gggaatgaag
      841 tcattgacgg catcgttaat cgcggtggat tgcagtcaat gatgtggtca atttcacttg
      901 ttatgattgc tcttacgctt ggcggggtga ttcaacggat aggtgttatt gatacactcc
      961 ttcaatccgc taacaagaaa attatcttca cgtggacatt tgattgcagc gactcaacat
     1021 cgtctatcgg agttaatgta gtaacaggag agcagtactt gtcaatatta ttgcctggga
     1081 aaacgtttga gtcattttat acaaagctta acgtagcaaa aaagaatctc tcaagaacac
     1141 ttgaggatgc agggacatta gtgaaccccc ttatcccttg gggagtaagc ggcgctttct
     1201 ttgcgagtac attaggggta gatgtgattg attacctacc gtttgctttc ttcttatttt
     1261 tgtctccatt tatgaccatt ttatttggct acctaggaat tggggtaggg aaagagcagt
     1321 ctcttgcaaa taaacgtgga taaagcaagt ggatttgatg caaagtaagg ataaggaggg
     1381 atgaccatgt ttagccgtct tatctcaatt gttagtttaa tcttgtcttt ttactttgct
     1441 tacaaatatc gctaccgtgt tattaatgca gtgctcggac gccgttggct gcggaaagtg
     1501 attattggtt ttgcgatgca gatcccgatg atcagagacc gtatgctagg atccgttctg
     1561 cagtctaatc gacctcaaaa tgtgtaacaa acaagaaagt ctgatgttca ctttaatgaa
     1621 agttaaagta tggcatcaga ctttttcgta tatatacata ctataaggct tattaatgtc
     1681 ctaaccttta gagcccttgt tatactataa gggataggag gattgtatct tgaaagtgaa
     1741 gtggagtacg atatgttttg atttagataa tacgttatat aaccatgagt atgcttttaa
     1801 gcgtgcgatt aaacaatgtt actatacaaa acttcagcaa tggaagatat ccgttgatca
     1861 cgctcctcca tttgaagcat ggtttactac atttaaatat tattgcgatc attttgggcc
     1921 cgaccttgaa cataagagga tgagcg
//