From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human pyruvate dehydrogenase beta subunit mRNA, complete cds.
Date: 29 Sep 92 10:06:59 GMT
Distribution: bionet
Checksum: 44375     5

LOCUS       HUMPDHB      1501 bp ss-mRNA            PRI       29-SEP-1992
DEFINITION  Human pyruvate dehydrogenase beta subunit mRNA, complete cds.
ACCESSION   J03576
KEYWORDS    pyruvate dehydrogenase.
SOURCE      Human foreskin fibroblast, cDNA to mRNA, clone pHPDB.
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 1501)
  AUTHORS   Koike,K., Ohta,S., Urata,Y., Kagawa,Y. and Koike,M.
  TITLE     Cloning and sequencing of cDNAs encoding alpha and beta subunits of
            human pyruvate dehydrogenase
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 85, 41-45 (1988)
  STANDARD  full staff_entry
REFERENCE   2  (bases 1 to 1501)
  AUTHORS   Koike,K., Urata,Y. and Koike,M.
  TITLE     Molecular cloning and characterization of human pyruvate
            dehydrogenase beta subunit gene
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 87, 5594-5597 (1990)
  STANDARD  full staff_entry
COMMENT
            [Unpublished (1990) 12-4, Sakamoto-machi, Nagasaki-shi, 852, Japan]
            revises [1].
            Draft entry and printed copy of sequence for [1] kindly provided by
            K.Koike, 11-APR-1988; and computer-readable copy for [Unpublished
            (1990) 12-4, Sakamoto-machi, Nagasaki-shi, 852, Japan],
            12-MAR-1990
FEATURES             Location/Qualifiers
     mRNA            <1..1501
                     /gene="PDHB"
     CDS             19..1098
                     /EC_number="1.2.4.1"
                     /product="pyruvate dehydrogenase beta subunit"
                     /gene="PDHB"
                     /codon_start=1
                     /translation="MAAVSGLVRRPLREVSGLLKRRFHWTAPAALQVTVRDAINQGMD
                     EELERDEKVFLLGEEVAQYDGAYKVSRGLWKKYGDKRIIDTPISEMGFAGIAVGAAMA
                     GLRPICEFMTFNFSMQAIDQVINSAAKTYYMSGGLQPVPIVFRGPNGASAGVAAQHSQ
                     CFAAWYGHCPGLKVVSPWNSEDAKGLIKSAIRDNNPVVVLENELMYGVPFEFLPEAQS
                     KDFLIPIGKAKIERQGTHITVVSHSRPVGHCLEAAAVLSKEGVECEVINMRTIRPMDM
                     ETIEASVMKTNHLVTVEGGWPQFGVGAEICARIMEGPAFNFLDAPAVRVTGADVPMPY
                     AKILEDNSIPQVKDIIFAIKKTLNI"
     sig_peptide     19..108
                     /gene="PDHB"
                     /codon_start=1
     mat_peptide     109..1095
                     /product="pyruvate dehydrogenase beta subunit"
                     /gene="PDHB"
                     /codon_start=1
BASE COUNT      421 a    291 c    374 g    415 t
ORIGIN
        1 atagaggaca cgaccaagat ggcggcggtg tctggcttgg tgcggagacc ccttcgggag
       61 gtctccgggc tgctgaagag gcgctttcac tggaccgcgc cggctgcgct gcaggtgaca
      121 gttcgtgatg ctataaatca gggtatggat gaggagctgg aaagagatga gaaggtattt
      181 ctgcttggag aagaagttgc ccagtatgat ggggcataca aggttagtcg agggctgtgg
      241 aagaaatatg gagacaagag gattattgac actcccatat cagagatggg ctttgctgga
      301 attgctgtag gtgcagctat ggctgggttg cggcccattt gtgaatttat gaccttcaat
      361 ttctccatgc aagccattga ccaggttata aactcagctg ccaagaccta ctacatgtct
      421 ggtggccttc agcctgtgcc tatagtcttc aggggaccca atggtgcctc agcaggtgta
      481 gctgcccagc actcacagtg ctttgctgcc tggtatgggc actgcccagg cttaaaggtg
      541 gtcagtccct ggaattcaga ggatgctaaa ggacttatta aatcagccat tcgggataac
      601 aatccagtgg tggtgctaga gaatgaattg atgtatgggg ttccttttga atttctcccg
      661 gaagctcagt caaaagattt tctgattcct attggaaaag ccaaaataga aaggcaagga
      721 acacatataa ctgtggtttc ccattcaaga cctgtgggcc actgcttaga agctgcagca
      781 gtgctatcta aagaaggagt tgaatgtgag gtgataaata tgcgtaccat tagaccaatg
      841 gacatggaaa ccatagaagc cagtgtcatg aagacaaatc atcttgtaac tgtggaagga
      901 ggctggccac agtttggagt aggagctgaa atctgtgcca ggatcatgga aggtcctgcg
      961 ttcaatttcc tggatgctcc tgctgttcgt gtcactggtg ctgatgtccc tatgccttat
     1021 gcaaagattc tagaggacaa ctctatacct caggtcaaag acatcatatt tgcaataaag
     1081 aaaacattaa atatttagtt tggacttgaa tatcaagtcg ttgaaattta tttgaaatac
     1141 ttgctggcac tgcacctgga tttgtactgc aagacctgac tattcataaa ggaaaacgat
     1201 ttctaaagca acagcaggta tttttgtaca gggaagttta aatgtgtttg tgtatggaaa
     1261 actctccact ctcctcccct agatgccatg cttccttttg tctgttacgg ttgccatgtt
     1321 ctttgaataa caaattatat cacattttat cctctctcac cacaaggaca aagtatggat
     1381 gtggcagagt cctgatgaaa gatgtatcca aacaagataa cttatatgta taaaattaaa
     1441 gcatataata cacatttact gttagtttgt tttgataagg aataaaggaa tttctaacat
     1501 g
//