From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Saccharomyces cerevisiae succinate dehydrogenase flavoprotein
Date: 28 Aug 92 10:01:25 GMT
Distribution: bionet
Checksum: 44339     8

LOCUS       YSCSDH1A     3905 bp ds-DNA             PLN       28-AUG-1992
DEFINITION  Saccharomyces cerevisiae succinate dehydrogenase flavoprotein
            subunit (SDH1) gene and debranching enzyme (DBR1) gene, complete
            cds.
ACCESSION   M86909
KEYWORDS    succinate dehydrogenase flavoprotein subunit.
SOURCE      Saccharomyces cerevisiae DNA.
  ORGANISM  Saccharomyces cerevisiae
            Eukaryota; Fungi; Eumycota; Hemiascomycetes; Endomycetales;
            Saccharomycetaceae.
REFERENCE   1  (bases 1 to 3905)
  AUTHORS   Chapman,K.B., Solomon,S.D. and Boeke,J.D.
  TITLE     SDH1, the gene encoding the succinate dehydrogenase flavoprotein
            subunit from Saccharomyces cerevisiae
  JOURNAL   Gene 118, 131-136 (1992)
  STANDARD  full staff_entry
FEATURES             Location/Qualifiers
     misc_structure  202..237
                     /gene="SDH1"
                     /note="putative"
     CDS             273..2195
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /gene="SDH1"
                     /note="putative"
                     /codon_start=1
                     /translation="MLSLKKSALSKLTLLRNTRTFTSSALVRQTQGSVNGSASRSADG
                     KYHIIDHEYDCVVIGAGGAGLRAAFGLAEAGYKTACISKLFPTRSHTVAAQGGINAAL
                     GNMHKDNWKWHMYDTVKGSDWLGDQDSIHYMTREAPKSIIELEHYGVPFSRTENGKIY
                     QRAFGGQTKEYGKGAQAYRTCAVADRTGHALLHTLYGQALRHDTHFFIEYFALDLLTH
                     NGEVVGVIAYNQEDGTIHRFRAHKTIIATGGYGRAYFSCTSAHTCTGDGNAMVSRAGF
                     PLQDLEFVQFHPSGIYGSGCLITEGARGEGGFLVNSEGERFMERYAPTAKDLACRDVV
                     SRAITMEIREGRGVGKKKDHMYLQLSHLPPEVLKERLPGISETAAIFAGVDVTKEPIP
                     IIPTVHYNMGGIPTKWNGEALTIDEETGEDKVIPGLMACGEAACVSVHGANRLGANSL
                     LDLVVFGRAVAHTVADTLQPGLPHKPLPSDLGKESIANLDKLRNANGSRSTAEIRMNM
                     KQTMQKDVSVFRTQSSLDEGVRNITAVEKTFDDVKTTDRSMIWNSDLVETLELQNLLT
                     CASQTAVSAANRKESRGAHAREDYPNRDDEHWMKHTLSWQKDVAAPVTLKYRRVIDHT
                     LDEKECPSVPPTVRAY"
     repeat_unit     2245..2256
                     /gene="SDH1"
                     /note="putative"
     repeat_unit     2264..2275
                     /gene="SDH1"
                     /note="putative"
     repeat_region   2346..2412
                     /gene="SDH1"
                     /note="putative"
     CDS             2573..3790
                     /product="debranching enzyme"
                     /gene="DBR1"
                     /note="putative"
                     /codon_start=1
                     /translation="MTKLRIAVQGCCHGQLNQIYKEVSRIHAKTPIDLLIILGDFQSI
                     RDGQDFKSIAIPPKYQRLGDFISYYNNEIEAPVPTIFIGGNHESMRHLMLLPHGGYVA
                     KNIFYMGYSNVIWFKGIRIGSLSGIWKEWDFNKQRPDWNDLENNNWKANIRNLYHVRI
                     SDIAPLFMIKHRIDIMLSHDWPNGVVYHGDTKHLLKLKPFFEQDIKEGKLGSPVTWQL
                     LRDLRPQWWLSAHLHVRFMASIKHNKRSHEPPNKSTSKTKKNNNEIDLDLSSDEDERS
                     GIMNCQEENEYDSKYGETRFLALDKCLPRRRWLEILEIEPDTSHASWKDENHRMFWDP
                     EFINNLVICQKNKNLLSNKPFNSVNWIELSQSNREEGRDIDWENYAIPAYTLDIQKDE
                     VRQTKAFISKFMT"
BASE COUNT     1186 a    753 c    824 g   1142 t
ORIGIN
        1 ggatccgggc ctcttctatt ggttgtttgt ttgctcaaac ccgttatata ttcaggcatc
       61 ctcgactcta accttttgcc acgtcgaggc ggcttgaagc ttaaatagca ctaataaact
      121 agtagacaca ttataactaa ccagtagtat actttgcact ttcgatattc ttttcactaa
      181 tctcctcccc aaccccttat tgaagataaa aagaaagaaa gaaagaaaga aaaaaatcca
      241 atttcatagt acgaagaaga acgagaataa agatgctatc gctaaaaaaa tcagcgctct
      301 ccaagttgac tttgctcaga aacacaagaa catttacatc gtcagctttg gtgcgccaaa
      361 cgcagggctc tgtaaacggt tccgcgtcca gatctgcaga cgggaagtac cacataatag
      421 atcacgagta tgactgtgtg gtaatcggtg ccggtggtgc cggccttaga gcggcctttg
      481 gtcttgccga ggcgggctac aagactgctt gtatatccaa gcttttcccc accagatccc
      541 acactgttgc tgctcagggt ggtatcaatg ccgctctggg aaatatgcac aaggataact
      601 ggaaatggca tatgtacgat actgtgaaag gatctgattg gctaggtgac caggactcca
      661 tccattacat gaccagggaa gcgcccaaat cgatcattga actggaacac tatggtgttc
      721 ctttttcaag aactgaaaac ggtaagatct accaaagagc ctttggtggt cagaccaagg
      781 agtacggtaa gggtgctcag gcctatagaa catgcgctgt cgcagacagg acaggacatg
      841 ctcttttaca cacgctttat ggccaagctt taagacatga cacacatttc tttattgagt
      901 actttgccct cgatctgttg acccataatg gcgaggtcgt tggtgtcatc gcttataatc
      961 aggaagacgg taccatccac agattcagag cacacaagac cattattgcc actggtggct
     1021 atggtagagc atacttctct tgtacctctg ctcacacatg tacgggtgac ggtaatgcca
     1081 tggtttcgcg tgctggtttc cccttgcaag atttagagtt tgttcaattc catccttcag
     1141 gtatatatgg gtctggttgc ttaatcactg aaggtgctcg tggtgaaggt ggttttttgg
     1201 ttaattctga aggtgaaaga ttcatggaac gttacgctcc tacggccaag gatctagctt
     1261 gtagagatgt cgtttccaga gcaatcacca tggagatcag agaaggcaga ggtgttggta
     1321 agaaaaagga ccacatgtac ttacaattga gccatctacc tccggaagtt ctaaaggaaa
     1381 gattgccagg tatctctgaa acagcagcca tttttgctgg tgtagacgtc accaaggaac
     1441 ctattcccat tattcctacc gtccactata acatgggtgg tattcccacg aagtggaatg
     1501 gtgaggcatt aaccattgat gaagaaactg gcgaagacaa ggttattccc ggtttaatgg
     1561 cttgtggtga agccgcttgt gtttctgtcc atggtgccaa tagattaggt gccaattcct
     1621 tgttggatct tgttgtcttt ggtcgtgctg ttgcccatac ggttgctgac actttacagc
     1681 ctgggttgcc acacaaacca ctaccttctg atttgggtaa agaatccatc gcaaacttgg
     1741 ataaactaag aaatgctaat ggttcaagat ctacggcaga aattagaatg aatatgaaac
     1801 aaactatgca aaaggatgtt tccgtcttta gaacacaatc atctttagat gaaggtgttc
     1861 ggaacattac tgcagtagag aagacctttg atgatgtgaa gacgaccgat agatcaatga
     1921 tctggaattc tgacttggtt gaaactctgg agctacagaa cttattaacc tgtgcctccc
     1981 aaacagctgt ttccgctgct aatagaaagg aatcccgtgg tgctcatgca agagaggatt
     2041 atccaaatag agatgacgaa cattggatga agcatacatt atcctggcaa aaggacgtcg
     2101 ctgccccagt gactttgaaa tacagaaggg ttatcgatca cactttggac gaaaaggaat
     2161 gtccttccgt acctccaact gtaagagcct actaatttga acctcattgt attttacgga
     2221 aaagaatatc atactcttct ttaattgcac tttttttgtg cgtttgcact tttttaccac
     2281 tgactcacta atttgtatat atacctatta atatacattt acataaagtt tcttcttata
     2341 catactctat ttatttagtt atttattaac ttactattta tttatttatt tatttattta
     2401 tttattactt tcaatttttt atcgaggcat ttccttagtt ctccaatttt ttttctcatt
     2461 agccagatgt gtgtttttct ggccctcaca aaaaatgagc aggagaaagt catatggcga
     2521 acgtaaatat gtaactaaaa attaagatgg gcagacattt atcattttgc ttatgactaa
     2581 attgcgaatt gctgtacaag ggtgctgtca tggtcagcta aaccaaattt ataaagaagt
     2641 gtcacgaatc catgcgaaga ctcccatcga tctattaatt attcttggag attttcaaag
     2701 tattcgtgat ggtcaggatt ttaagtcaat agccatacca ccaaaatatc aaagactcgg
     2761 tgatttcata tcatactaca ataatgagat tgaagcccca gtccctacta tttttattgg
     2821 cggtaatcat gaatcgatga gacatttaat gcttctgcca catggtggtt atgtagcaaa
     2881 gaacattttt tatatgggat actctaacgt tatatggttt aaaggcataa ggataggttc
     2941 attgagcggg atatggaaag agtgggattt caataagcaa cgaccagatt ggaatgacct
     3001 agaaaataac aattggaaag ctaacataag aaatctgtat catgtaagaa tttctgatat
     3061 tgctcctctt tttatgatca agcatcgtat tgatattatg ctgagccacg attggcccaa
     3121 tggagtcgtt tatcatggcg atactaagca cttgttgaag cttaagcctt tttttgagca
     3181 agatattaaa gagggaaaac taggcagccc agtgacctgg caactactaa gagatttgcg
     3241 gcctcaatgg tggttgagcg cccatttgca tgtcaggttc atggcctcca ttaagcataa
     3301 taaaagaagc cacgaaccgc ctaataagtc aacttcaaag acaaaaaaaa acaataatga
     3361 aatagatttg gatttatcca gtgacgagga cgaaaggtct ggtattatga attgccaaga
     3421 agagaatgag tatgattcca aatacggcga gacacggttc ttggcgttgg acaagtgcct
     3481 gccaagaaga cgttggttag aaattctgga aatagaaccg gatacatcgc atgcttcgtg
     3541 gaaggacgag aatcaccgta tgttctggga tcctgaattt atcaataatt tggtaatttg
     3601 tcaaaaaaat aagaatctcc taagcaataa accgtttaat agcgtcaatt ggatagaact
     3661 gtctcaatct aatagagagg aaggaaggga tattgattgg gaaaattatg ccattcctgc
     3721 atacacctta gatattcaga aagatgaagt acgacaaact aaagcattta tctctaaatt
     3781 catgacttga gagatatttc tattacgaat gcagacggaa ttagagctta aagccaagcc
     3841 ttcttgagag aatttttagt catacacatg aatatatata tattttttat atatttacta
     3901 atttg
//