From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: H.sapiens si mRNA for sucrase-isomaltase
Date: 10 Sep 92 10:33:01 GMT
Distribution: bionet
Checksum: 00769    12

LOCUS       HUMSUISO     6021 bp ss-RNA             PRI       10-SEP-1992
DEFINITION  H.sapiens si mRNA for sucrase-isomaltase
ACCESSION   X63597
KEYWORDS    isomaltase; pro-sucrase-isomaltase; si gene; sucrase;
            sucrase-isomaltase.
SOURCE      Homo sapiens RNA.
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 6021)
  AUTHORS   Chantret,I., Lacasa,M., Chevalier,G., Ruf,J., Islam,I., Mantei,N.,
            Edwards,Y., Swallow,D. and Rousset,M.
  TITLE     Sequence of the complete cDNA and the 5' structure of the human
            sucrase-isomaltase gene. possible homology with yeast glucoamylase
  JOURNAL   Biochem. J. 285, 915-923 (1992)
  STANDARD  full automatic
COMMENT     *source: tissue=intestine; *source: chromosome=3q25-26;
            See also M22616
            From EMBL    entry HSSUISO;  dated 18-AUG-1992.
FEATURES             Location/Qualifiers
     misc_feature    63..2855
                     /note="isomaltase"
     CDS             63..5546
                     /EC_number="3.2.1.10"
                     /EC_number="3.2.1.48"
                     /product="prosucrose-isomaltase"
                     /gene="si"
                     /codon_start=1
                     /translation="MARKKFSGLEISLIVLFVIVTIIAIALIVVLATKTPAVDEISDS
                     TSTPATTRVTTNPSDSGKCPNVLNDPVNVRINCIPEQFPTEGICAQRGCCWRPWNDSL
                     IPWCFFVDNHGYNVQDMTTTSIGVEAKLNRIPSPTLFGNDINSVLFTTQNQTPNRFRF
                     KITDPNNRRYEVPHQYVKEFTGPTVSDTLYDVKVAQNPFSIQVIRKSNGKTLFDTSIG
                     PLVYSDQYLQISARLPSDYIYGIGEQVHKRFRHDLSWKTWPIFTRDQLPGDNNNNLYG
                     HQTFFMCIEDTSGKSFGVFLMNSNAMEIFIQPTPIVTYRVTGGILDFYILLGDTPEQV
                     VQQYQQLVGLPAMPAYWNLGFQLSRWNYKSLDVVKEVVRRNREAGIPFDTQVTDIDYM
                     EDKKDFTYDQVAFNGLPQFVQDLHDHGQKYVIILDPAISIGRRANGTTYATYERGNTQ
                     HVWINESDGSTPIIGEVWPGLTVYPDFTNPNCIDWWANECSIFHQEVQYDGLWIDMNE
                     VSSFIQGSTKGCNVNKLNYPPFTPDILDKLMYSKTICMDAVQNWGKQYDVHSLYGYSM
                     AIATEQAVQKVFPNKRSFILTRSTFAGSGRHAAHWLGDNTASWEQMEWSITGMLEFSL
                     FGIPLVGADICGFVAETTEELCRRWMQLGAFYPFSRNHNSDGYEHQDPAFFGQNSLLV
                     KSSRQYLTIRYTLLPFLYTLFYKAHVFGETVARPVLHEFYEDTNSWIEDTEFLWGPAL
                     LITPVLKQGADTVSAYIPDAIWYDYESGAKRPWRKQRVDMYLPADKIGLHLRGGYIIP
                     IQEPDVTTTASRKNPLGLIVALGENNTAKGDFFWDDGETKDTIQNGNYILYTFSVSNN
                     TLDIVCTHSSYQEGTTLAFQTVKILGLTDSVTEVRVAENNQPMNAHSNFTYDASNQVL
                     LIADLKLNLGRNFSVQWNQIFSENERFNCYPDADLATEQKCTQRGCVWRTGSSLSKAP
                     ECYFPRQDNSYSVNSARYSSMGITADLQLNTANARIKLPSDPISTLRVEVKYHKNDML
                     QFKIYDPQKKRYEVPVPLNIPTTPISTYEDRLYDVEIKENPFGIQIRRRSSGRVIWDS
                     WLPGFAFNDQFIQISTRLPSEYIYGFGEVEHTAFKRDLNWNTWGMFTRDQPPGYKLNS
                     YGFHPYYMALEEEGNAHGVFLLNSNAMDVTFQPTPALTYRTVGGILDFYMFLGPTPQV
                     ATKQYHEVIGHPVMPAYWALGFQLCRYGYANTSEVRELYDAMVAANIPYDVQYTDIDY
                     MERQLDFTIGEAFQDLPQFVDKIRGEGMRYIIILDPAISGNETKTYPAFERGQQNDVF
                     VKWPNTNDICWAKVWPDLPNITIDKTLTEDEAVNASRAHVAFPDFFRTSTAEWWAREI
                     VDFYNEKMKFDGLWIDMNEPSSFVNGTTTNQCRNDELNYPPYFPELTKRTDGLHFRTI
                     CMEAEQILSDGTSVLHYDVHNLYGWSQMKPTHDALQKTTGKRGIVISRSTYPTSGRWG
                     GHWLGDNYARWDNMDKSIIGMMEFSLFGISYTGADICGFFNNSEYHLCTRWMQLGAFY
                     PYSRNHNIANTRRQDPASWNETFAEMSRNILNIRYTLLPYFYTQMHEIHANGGTVIRP
                     LLHEFFDEKPTWDIFKQFLWGPAFMVTPVLEPYVQTVNAYVPNARWFDYHTGKDIGVR
                     GQFQTFNASYDTINLHVRGGHILPCQEPAQNTFYSRQKHMKLIVAADDNQMAQGSLFW
                     DDGESIDTYERDLYLSVQFNLNQTTLTSTILKRGYINKSETRLGSLHVWGKGTTPVNA
                     VTLTYNGNKNSLPFNEDTTNMILRIDLTTHNVTLEEPIEINWS"
     misc_feature    2856..5546
                     /note="sucrase"
     polyA_signal    5992..5997
     polyA_site      6009
BASE COUNT     1962 a   1127 c   1170 g   1762 t
ORIGIN
        1 tattttggca gccttatcca agtctggtac aacatagcaa agagaacagg ctatgaaata
       61 agatggcaag aaagaaattt agtggattgg aaatctctct gattgtcctt tttgtcatag
      121 ttactataat agctattgcc ttaattgttg ttttagcaac taagacacct gctgttgatg
      181 aaattagtga ttctacttca actccagcta ctactcgtgt gactacaaat ccttctgatt
      241 caggaaaatg tccaaatgtg ttaaatgatc ctgtcaatgt gagaataaac tgcattccag
      301 aacaattccc aacagaggga atttgtgcac agagaggctg ctgctggagg ccgtggaatg
      361 actctcttat tccttggtgc ttcttcgttg ataatcatgg ttataacgtt caagacatga
      421 caacaacaag tattggagtt gaagccaaat taaacaggat accttcacct acactatttg
      481 gaaatgacat caacagtgtt ctcttcacaa ctcaaaatca gacacccaat cgtttccggt
      541 tcaagattac tgatccaaat aatagaagat atgaagttcc tcatcagtat gtaaaagagt
      601 ttactggacc cacagtttct gatacgttgt atgatgtgaa ggttgcccaa aacccattta
      661 gcatccaagt tattaggaaa agcaacggta aaactttgtt tgacaccagc attggtccct
      721 tagtgtactc tgaccagtac ttacagatct cagcccgtct tccaagtgat tatatttatg
      781 gtattggaga acaagttcat aagagatttc gtcatgattt atcctggaaa acatggccaa
      841 tttttactcg agaccaactt cctggtgata ataataataa tttatacggc catcaaacat
      901 tctttatgtg tattgaagat acatctggaa agtcattcgg tgttttttta atgaatagca
      961 atgcaatgga gatttttatc cagcctactc caatagtaac atatagagtt accggtggca
     1021 ttctggattt ttacatcctt ctaggagata caccagaaca agtagttcaa cagtatcaac
     1081 agcttgttgg actaccagca atgccagcat attggaatct tggattccaa ctaagtcgct
     1141 ggaattataa gtcactagat gtagtgaaag aagtggtaag gagaaaccgg gaagctggca
     1201 taccatttga tacacaggtc actgatattg actacatgga agacaagaaa gactttactt
     1261 atgatcaagt tgcgtttaac ggactccctc aatttgtgca agatttgcat gaccatggac
     1321 agaaatatgt catcatcttg gaccctgcaa tttccatagg tcgacgtgcc aatggaacaa
     1381 catatgcaac ctatgagagg ggaaacacac aacatgtgtg gataaatgag tcagatggaa
     1441 gtacaccaat tattggagag gtatggccag gattaacagt ataccctgat ttcactaatc
     1501 caaactgcat tgattggtgg gcaaatgaat gcagtatttt ccatcaagaa gtgcaatatg
     1561 atggactttg gattgacatg aatgaagttt ccagctttat tcaaggttca acaaaaggat
     1621 gtaatgtaaa caaattgaat tatccaccgt ttactcctga tattcttgac aaactcatgt
     1681 attccaaaac aatttgcatg gatgctgtgc agaactgggg taaacagtat gatgttcata
     1741 gcctctatgg atacagcatg gctatagcca cagagcaagc tgtacaaaaa gtttttccta
     1801 ataagagaag cttcattctt acccgctcaa catttgctgg atctggaaga catgctgctc
     1861 attggttagg agacaatact gcttcatggg aacaaatgga atggtctata actggaatgc
     1921 tggagttcag tttgtttgga atacctttgg ttggagcaga catctgtgga tttgtggctg
     1981 aaaccacaga agaactttgc agaagatgga tgcaacttgg ggcattttat ccattttcca
     2041 gaaaccataa ttctgacgga tatgaacatc aggatcctgc attttttggg cagaattcac
     2101 ttttggttaa atcatcaagg cagtatttaa ctattcgcta caccttatta cccttcctct
     2161 acactctgtt ttataaagcc catgtgtttg gagaaacagt agcaagacca gttcttcatg
     2221 agttttatga ggatacgaac agctggattg aggacactga gtttttgtgg ggccctgcat
     2281 tacttattac tcctgttcta aaacagggag cagatactgt gagtgcctac atccctgatg
     2341 ctatttggta tgattatgaa tctggtgcaa aaaggccatg gaggaaacaa cgggttgata
     2401 tgtatcttcc agcagacaaa ataggattac atcttagagg aggttatatc atccccattc
     2461 aagaaccaga tgtaacaaca acagcaagcc gtaagaatcc tctaggactt atagtcgcat
     2521 taggtgaaaa caacacagcc aaaggagact ttttctggga tgatggagaa actaaagata
     2581 caatacaaaa tggcaactac atattatata cattttcagt ttctaataac acattagata
     2641 ttgtgtgcac acattcatca tatcaggaag gaactacctt agcatttcag actgtaaaaa
     2701 tccttgggtt gacagacagt gttacagaag ttagagtggc ggaaaataat caaccaatga
     2761 acgctcattc caatttcact tatgatgctt ctaaccaggt tctcctaatt gcagatctca
     2821 aacttaatct tggaagaaac tttagtgttc aatggaatca aattttctca gaaaatgaaa
     2881 gatttaattg ttatccagat gcagatttgg caactgaaca aaagtgcaca caacgtggct
     2941 gtgtatggag aacgggttct tctctatcca aagcacctga gtgttacttt cccagacaag
     3001 ataactctta ttcagtcaac tcagctcgct attcatccat gggtataaca gctgacctcc
     3061 aactaaatac tgcaaatgcc agaataaagt taccttctga ccccatctca actcttcgtg
     3121 tggaggtgaa atatcacaaa aatgatatgt tgcagtttaa gatttatgat ccccaaaaga
     3181 agagatatga agtaccagta ccgttaaaca ttccaaccac cccaataagt acttatgaag
     3241 acagacttta tgatgtggaa atcaaggaaa atccttttgg catccagatt cgacggagaa
     3301 gcagtggaag agtcatttgg gattcttggc tgcctggatt tgcttttaat gaccagttca
     3361 ttcaaatatc gactcgcctg ccatcagaat atatatatgg ttttggggaa gtggaacata
     3421 cagcatttaa gcgagatctg aactggaata cttggggaat gttcacaaga gaccaacccc
     3481 ctggttacaa acttaattcc tatggatttc atccctatta catggctctg gaagaggagg
     3541 gcaatgctca tggtgttttc ttactcaaca gcaatgcaat ggatgttaca ttccagccaa
     3601 ctcctgctct aacttaccgt acagttggag ggatcttgga tttttatatg tttttgggcc
     3661 caactccaca agttgcaaca aagcaatacc atgaagtaat tggccatcca gtcatgccag
     3721 cttattgggc tttgggattc caattatgtc gttatggata tgcaaatact tcagaggttc
     3781 gggaattata tgacgctatg gtggctgcta acatccccta tgatgttcag tacacagaca
     3841 ttgactacat ggaaaggcag ctagacttta caattggtga agcattccag gaccttcctc
     3901 agtttgttga caaaataaga ggagaaggaa tgagatacat tattatcctg gatccagcaa
     3961 tttcaggaaa tgaaacaaag acttaccctg catttgaaag aggacagcag aatgatgtct
     4021 ttgtcaaatg gccaaacacc aatgacattt gttgggcaaa ggtttggcca gatttgccca
     4081 acataacaat agataaaact ctaacggaag atgaagctgt taatgcttcc agagctcatg
     4141 tagctttccc agatttcttc aggacttcca cagcagagtg gtgggccaga gaaattgtgg
     4201 acttttacaa tgaaaagatg aagtttgatg gtttgtggat tgatatgaat gagccatcaa
     4261 gttttgtaaa tggaacaact actaatcaat gcagaaatga cgaactaaat tatccacctt
     4321 atttcccaga actcacaaaa agaactgatg gattacattt cagaacaatt tgcatggaag
     4381 ctgagcagat tcttagtgat ggaacatcag ttttgcatta cgatgttcac aatctctatg
     4441 gatggtcaca gatgaaacct actcatgatg cattgcaaaa gacaactgga aaaagaggga
     4501 ttgtaatttc tcgttccacg tatcctacta gtggacgatg gggaggacac tggcttggag
     4561 acaactatgc acgatgggac aacatggaca aatcaatcat tggtatgatg gaatttagtc
     4621 tgtttggaat atcatatact ggagcagaca tctgtggttt tttcaacaac tcagaatatc
     4681 atctctgtac ccgctggatg caacttggag cattttatcc atactcaagg aatcacaaca
     4741 ttgcaaatac tagaagacaa gatcccgctt cctggaatga aacttttgct gaaatgtcaa
     4801 ggaatattct aaatattaga tacaccttat tgccctattt ttacacacaa atgcatgaaa
     4861 ttcatgctaa tggtggcact gttatccgac cccttttgca tgagttcttt gatgaaaaac
     4921 caacctggga tatattcaag cagttcttat ggggtccagc atttatggtt accccagtac
     4981 tggaacctta tgttcaaact gtaaatgcct acgtccccaa tgctcggtgg tttgactacc
     5041 atacaggcaa agatattggc gtcagaggac aatttcaaac atttaatgct tcttatgaca
     5101 caataaacct acatgtccgt ggtggtcaca tcctaccatg tcaagagcca gctcaaaaca
     5161 cattttacag tcgacaaaaa cacatgaagc tcattgttgc tgcagatgat aatcagatgg
     5221 cacagggttc tctgttttgg gatgatggag agagtataga cacctatgaa agagacctat
     5281 atttatctgt acaatttaat ttaaaccaga ccaccttaac aagcactata ttgaagagag
     5341 gttacataaa taaaagtgaa acgaggcttg gatcccttca tgtatggggg aaaggaacta
     5401 ctcctgtcaa tgcagttact ctaacgtata acggaaataa aaattcgctt ccttttaatg
     5461 aagacactac caacatgata ttacgtattg atctgaccac acacaatgtt actctagaag
     5521 aaccaataga aatcaactgg tcatgaagat caccatcaat tttagttgtc aatgggaaaa
     5581 aacaccagga tttaagtttc acagcactta caattttccc tcttcacttg gttcttgtac
     5641 tctacaaaat atagctttca taacatcgaa aagttatttt gtagcgtaca tcaatgataa
     5701 tgctaatttt attatagtaa tgtgacttgg attcaatttt aaggcatatt taacaaaatt
     5761 tgaatagccc tatttatcct tgttaagtat cagctacaat tgtaaactag ttactaaaca
     5821 tgtatgtaaa tagctaagat ataatttaaa cgtgattttt aaattaaata aaatttttat
     5881 gtaattatat atactatatt tttctcaatg tttagcagat ttaagatatg taacaacaat
     5941 tatttgaaga tttaattact tcttagtatg tgcatttaat tagaaaaaga gaataaaaaa
     6001 tgtaagtgta aaaaaaaaaa a
//