From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Pseudomonas syringae pathovar envelope protein (hrpH) gene,
Date: 4 Sep 92 10:05:36 GMT
Distribution: bionet
Checksum: 15192     6

LOCUS       PSEHRPH      2580 bp ds-DNA             BCT       04-SEP-1992
DEFINITION  Pseudomonas syringae pathovar envelope protein (hrpH) gene,
            complete cds.
ACCESSION   L01064
KEYWORDS    envelope protein; hrpH gene.
SOURCE      Pseudomonas syringae (strain 61) DNA.
  ORGANISM  Pseudomonas syringae
            Prokaryotae; Gracilicutes; Scotobacteria; Pseudomonadaceae.
REFERENCE   1  (bases 1 to 2580)
  AUTHORS   Huang,H.-C., He,S.Y., Bauer,D.W. and Collmer,A.
  TITLE     The Pseudomonas syringae pv. syringae 61 hrpH product: an envelope
            protein required for elicitation of the hypersensitive response in
            plants
  JOURNAL   J. Bacteriol. (1992) In press
  STANDARD  full staff_entry
FEATURES             Location/Qualifiers
     misc_signal     118..132
                     /note="harp box"
                     /gene="hrpH"
     promoter        176..188
                     /standard_name="-24/-12 promoter"
                     /gene="hrpH"
     RBS             230..234
                     /standard_name="Shine-Dalgarno"
                     /gene="hrpH"
     CDS             241..2487
                     /function="protein translocation; elicits hypersensitivity
                     in plants"
                     /product="envelope protein"
                     /gene="hrpH"
                     /codon_start=1
                     /translation="MRKALMWLPLLLIGLSPATWAVTPEAWKHTAYAYDARQTELATA
                     LADFAKEFGMALDMPPIPGVLDDRIRAQSPEEFLDRLGQEYHFQWFVYNDTLYVSPSS
                     EHTSARIEVSSDAVDDLQTALTDVGLLDKRFGWGVLPNEGVVLVRGPAKYVELVRDYS
                     KKVEAPEKGDKQDVIVFPLKYASAADRTIRYRDQQLVVAGVASILQDLLDTRSHGGSI
                     NGMDLLGRGGRGNGLAGGGSPDTPSLPMSSSGLDTNALEQGLDQVLHYGGGGTKSSGK
                     SRSGGRANIRVTADVRNNAVLIYDLPSRKAMYEKLIKELDVSRNLIEIDAVILDIDRN
                     ELAELSSRWNFNAGSVNGGANMFDAGTSSTLFIQNAGKFAAELHALEGNGSASVIGNP
                     SILTLENQPAVIDFSRTEYLTATSERVANIEPITAGTSLQVTPRSLDHDGKPQVQLIV
                     DIEDGQIDISDINDTQPSVRKGNVSTQAVIAEHGSLVIGGFHGLEANDKVHKVPLLGD
                     IPYIGKLLFQSRSRELSQRERLFILTPRLIGDQVNPARYVQNGNPHDVDDQMKRIKER
                     RDGGELPTRGDIQKVFTQMVDGAAPEGMHDGETLPFETDSLCDPGQGLSLDGQRSQWY
                     ARKDWGVAVVVARNNTDKPVRIDESRCGGRWVIGVAAWPHAWLQPGEESEVYIAYVSH
                     RYRKWPKRAGHHCSGGQSHEDQQRCSRVGGVRYPDRVRHPRLFGNCLQTPGLHQPRTG
                     HLVASGHA"
BASE COUNT      537 a    772 c    788 g    483 t
ORIGIN
        1 cggtgccagc ctggcgtgtg cctgtgaccg gcgcgttcgc catcgatccg caaacgcgct
       61 gcatggtgct ggtcagctgg atgcctggcc cctgcggcct tgccgatctg ctgaaccgtc
      121 tggaaagcct ggccaatcag cgcgcggcct tgctcagcct gatgcacacc actctggtca
      181 attccgtgcc cgccttagcc gggcgcacga ccctcaatca ccggcaaccg ggagtttgaa
      241 atgcgcaagg ccttgatgtg gttgccttta ttgttgatcg ggctgtcccc cgccacgtgg
      301 gcggtgaccc ccgaagcctg gaaacatacg gcttacgcct acgatgcccg gcagaccgag
      361 ctggcgacgg cgctggccga cttcgccaag gaattcggca tggcgctgga catgccgccc
      421 atcccgggag tcctcgatga ccggatacgc gcccagagcc cggaagagtt tctcgatcgt
      481 ctgggccagg aatatcactt ccagtggttt gtgtataacg acacgttgta cgtcagcccc
      541 tccagcgagc acacctcggc acgcatcgaa gtgtcctccg acgcggtgga tgacctgcag
      601 actgccttga ccgatgtggg gctgctggac aagcgcttcg gttggggggt attgccgaac
      661 gaaggcgtgg ttctggttcg cggcccggca aaatatgtgg aactggtgcg cgattacagc
      721 aagaaagtcg aagcgccgga aaagggcgac aaacaggacg tcatcgtatt tccgctcaag
      781 tacgccagtg ctgcggaccg caccattcgc tatcgcgatc aacaactggt ggtcgccggt
      841 gtcgccagca ttctccagga tttgctggat acccgttctc atggcgggtc catcaatggt
      901 atggatctgc tggggcgagg cggtcgcggt aacggtctgg cgggcggtgg cagcccggac
      961 actccgagtc tgccaatgag ctccagcggc ctcgatacca atgcgttgga gcagggcctc
     1021 gatcaggtgc tgcactatgg tggcggcggc acgaaaagca gtggaaaaag tcgctctggc
     1081 gggcgggcca acatccgtgt aaccgccgat gtccgtaaca atgccgtgct gatctacgac
     1141 ttgccctcgc gcaaggccat gtacgaaaag ctcatcaaag agctggatgt ttcgcgcaac
     1201 ctgatcgaaa tcgacgccgt gattctcgac atcgatcgca acgagctggc agaactgtcc
     1261 agccgctgga acttcaatgc cgggagtgtc aacgggggcg ccaacatgtt cgacgctggc
     1321 accagctcca ccctgttcat tcagaacgcc ggcaagttcg ccgccgaact gcatgcgctg
     1381 gaaggtaacg gctcggcgtc tgtgatcggc aacccgtcca ttcttaccct tgaaaaccag
     1441 ccggccgtga tcgacttcag tcgcaccgaa tacctcaccg cgacctcgga acgcgtggcc
     1501 aatatcgagc ccatcaccgc aggcaccagt ctgcaggtga ctccgcgctc gctggatcac
     1561 gatggcaagc ctcaggtgca actgatcgtc gatatcgagg atggtcagat cgacatctcc
     1621 gacatcaatg acacgcagcc aagtgtgcgc aaaggcaatg tcagtaccca agccgtcata
     1681 gccgagcacg gttcgctggt catcggtggt ttccatggtc ttgaagccaa cgacaaggtc
     1741 cacaaggttc cgttgctggg cgacattccc tacatcggca agctgctgtt ccagtcacgc
     1801 agccgtgaac tgagccagcg cgagcgcctg ttcatcctga cgccaagact gattggcgat
     1861 caggtcaacc cggctcgcta tgtgcagaac ggcaaccctc acgacgtcga tgatcagatg
     1921 aaaaggatca aggaacggcg tgacggcggt gagctgccta cccgtggcga cattcagaaa
     1981 gtcttcacgc agatggttga tggcgctgcg ccagagggca tgcatgatgg cgaaacactg
     2041 ccctttgaaa ccgacagcct gtgtgacccg ggccagggcc tgagcctcga tggccagcgc
     2101 tcgcaatggt atgcccgcaa ggactggggt gtggctgtcg tggtcgcgcg caacaatacc
     2161 gacaaaccgg tgcggatcga cgagagtcgc tgcggtggtc gctgggtcat cggcgtcgcc
     2221 gcctggcccc atgcatggct gcaaccgggc gaggaaagcg aagtgtatat cgcgtacgtc
     2281 agccacagat atcgaaaatg gccaaagaga gccggccatc actgctccgg ggggcaaagc
     2341 catgaagatc agcagcgttg cagtcgtgtt ggtggtgttc gctaccctga ccgggtgcgc
     2401 cacccacggt tgttcgggaa ctgcctgcaa acgcccggac tccaccagcc gcgaactggt
     2461 catctggtgg cctccggaca tgcgtgacgg tcttgacgac caggaccacg agcgtgatta
     2521 caccgtcgtg aagttaaggg actagacatg atcgaggtcg aggagaaaac agcgttctac
//