From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Aspergillus flavus elastase gene, complete cds.
Date: 10 Jan 93 05:45:20 GMT
Distribution: bionet
Checksum: 46221 6

LOCUS       ASNELSTS     2955 bp ds-DNA             PLN       09-JAN-1993
DEFINITION  Aspergillus flavus elastase gene, complete cds.
ACCESSION   L08473
KEYWORDS    elastase.
SOURCE      Aspergillus flavus (strain 13) DNA.
  ORGANISM  Aspergillus flavus
            Eukaryota; Fungi; Eumycota; Ascomycotina; Plectomycetes;
            Eurotiales; Trichocomaceae.
REFERENCE   1  (bases 1 to 2955)
  AUTHORS   Kolattukudy,P.E., Matur,R.V. and Sirakova,T.
  TITLE     Elastase of Aspergillus flavus: Protein characterization and
            cloning, sequencing of cDNA and the gene
  JOURNAL   Unpublished (1993)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     sig_peptide     join(706..1029,1091..1129)
                     /codon_start=1
     RBS             694..698
     exon            706..1029
                     /number=1
     intron          1030..1090
                     /number=1
     exon            1091..1534
                     /number=2
     intron          1535..1597
                     /number=2
     exon            1597..1686
                     /number=3
     intron          1687..1744
                     /number=3
     exon            1745..2098
                     /number=4
     CDS             join(706..1029,1091..1534,1597..1686,1745..2098)
                     /product="elastase"
                     /codon_start=1
                     /translation="MQSIKRTLLLLGAVLPAVLAGPIFPHRRAPTTIPGKYIVTFKSD
                     VDQAAIDKHTAWATDIHKRNLQRRDSSEEDLPIGIERNFKINKFAAYSGSFDEDTIAQ
                     IRQSDEVAAVEEDQVWHLFDLTTQSDAPWGLGSISHKGQPSTDYIYDTNGGEGTYAYV
                     VDIGINVDHEEFEGRASLAYHAAGGQHVDGVGHGTHVSGTIGGKTYGVAKKANLLSVK
                     VFVGESSSTSIILDGFNWAANDIVSKKRTGKAAINMSLGGGYSKAFNDAVENAFNEGV
                     LSIVAAGNENTDASRTSPASAPDAFTVAAINVNNTRAYFSNYGSVVDIFAPGQNILSA
                     WIGSNTATNTISGTSMATPHIVGLSIYLMSLEVLSSPKAVSDRIKELATRGVVSNVAG
                     SPNLLAYNGNA"
BASE COUNT      634 a    856 c    817 g    648 t
ORIGIN      
        1 agttctagaa agccccctgg tctcccgctg gtctctatat ccaatcatcc agaacgcgac
       61 gtgataggca aggcaaaaga cgcgggcagc agcggatatc cggtgagttg ccaagcacct
      121 gagaaagatt aaggtcacat tgatgccaaa gacaaggcca gcccatccac tctgtagggc
      181 tcgtttgcgt gatgctgggc ccccagcaat gacgtcgatt tgctctggga gcgacgcatg
      241 ttcatgactt gcttctgggc caacgcagct gcaagtgtcg atcgcccatg ctcctcaggg
      301 actcgggctt ggcaaagcag cagctccgat aaccatgtgt gcatggtctg cggatggcct
      361 atgaaatgtc ttggaaggca gtcggccttc cgtgatgatc gccgttgaca tccacattgt
      421 ccctttcatt tacagcttct ccaataatag cgccaagtcc gacggagcac aatggagtcg
      481 tctgaggatg caatgtcacc tgtgagtttg gaacaccctc taggtacacc gagtcttggg
      541 cacgcgtgaa ctgcgacttt gcatggcgga gatggcaaat ggacatggtc tcgtgaaggg
      601 tatatagggc ggctcgccct caccttctgt cgtgcttctc ctcatcaagc cactgagata
      661 tctcaagctt aactcttctt tgcaaaccac tccgggcatc tcaccatgca gtccatcaag
      721 cgcactctct tgctcctggg agctgtcctt cccgcggtac tggccggtcc aatctttcca
      781 caccgtcgcg cgcctaccac tattcctggc aagtacattg tcaccttcaa gtcggatgtc
      841 gaccaggccg cgattgacaa gcacaccgcg tgggcgaccg atatccacaa gcgcaacctg
      901 cagcggcgcg actcgtccga ggaggacctc cccatcggca ttgagcggaa cttcaagatc
      961 aacaagtttg ccgcgtactc gggctccttt gatgaggata ccatcgcgca gattcgccag
     1021 agcgacgagg ttggtgacat tgcttgcggt gatcaacaag acgaatgaaa atcgaatcta
     1081 cggatcatag gtcgccgcgg ttgaggaaga ccaggtctgg cacctgttcg acctgaccac
     1141 ccagtcggac gccccctggg gattgggaag catctcccac aagggtcagc ccagcacgga
     1201 ctacatctat gacaccaacg gcggcgaggg cacctacgct tacgtcgtag acattggcat
     1261 caacgtggac cacgaggaat ttgagggccg tgcgagtctc gcgtaccacg ctgccggtgg
     1321 ccagcatgtg gacggtgtcg gccatggcac ccacgtttct ggtaccattg gtggtaagac
     1381 atacggcgtg gccaagaagg ccaacctgct gtcggtcaag gtcttcgtcg gggaatcgag
     1441 cagcacatcc atcatcctgg acggcttcaa ctgggcggcc aatgatattg tttccaagaa
     1501 gcgcactgga aaggcagcga tcaacatgag cttgggtatg ttatctgcca tctatgtgaa
     1561 tgatatcatt ggagtcacac agctgacgca agcataggcg gtggatactc caaggccttc
     1621 aatgatgccg tcgagaacgc ttttaacgag ggagtcctgt ccatcgtcgc cgccggcaat
     1681 gagaatgtgc gtgccacgtt cccccctgtg aatggagtgc ggactccgct gactgattcg
     1741 acagaccgac gcctcgcgca ccagcccggc ttctgctcct gatgccttta ccgtcgctgc
     1801 gatcaacgtg aacaacaccc gtgcctattt ctccaactac ggctccgtgg tggatatctt
     1861 cgccccgggc cagaacatcc tctctgcctg gatcggctcc aacacggcca ccaacaccat
     1921 ttcgggcact tccatggcca ccccccacat tgtcggcctg tccatctatc tgatgtcgct
     1981 ggaggtcctc agcagcccca aggccgtcag tgaccgcatc aaggagctgg cgactcgagg
     2041 cgtcgtcagc aacgtcgccg gtagccccaa cctgttggcg tacaacggca acgcctaacc
     2101 tcggcaagac ttggcatagc gcacgtgacc gcgtcgactt gggatatgga agccagcagg
     2161 tgatgtgatc ttagcttcaa tgtacatgta ctatactagc atcgcttctc gaggatcaac
     2221 gtggaagtta tttgacttgt ccgatacaac ctcctattac atctcccgtt ctctttaaac
     2281 taggcacatg gccccgtata tcccgcataa cctgtttggc gccgagcacc tgcagaggcc
     2341 tagtatgcgc catatctcga gctatcatca cgtttctccg ccggatgccc gctccggaat
     2401 tccaacgtgc gtcaggaagg tctcgacggt cattacggtt ggttcgcaga cgtcgcagac
     2461 gcagcgcagg taaggaaggg atagcaggtc tgggatcttc acggcggcgg cgaccattag
     2521 gcgaatgagt tgtccgtcgc gcggaatgtg gaccggggac attgcgtcga gagcacgata
     2581 tagccaccac cgggaaggac ggcagacttg ccagacacgg gggtctccag tcgcagctca
     2641 tacgtactta gagctgtgaa atgcatcacg agctccttgg aggtcttgga gatccgccat
     2701 gacgacgacc agcctagagg gtcgagcccg tggacctgct ggggggagga tctgcacgtc
     2761 gaccgggccc ctttacattc tgatgcaaag tttgccggct ggggagttat cgttgcgcaa
     2821 tccgctcgtc gttgtgcagg tctgggttaa agccgatgtc atacgctgcg tcggatgtga
     2881 tatcaacgct ggggagttca ttctgggcgt tctcctcgtg caggtttctt gttcttctgt
     2941 ctatagccgt ctaga
//