From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Synthetic rpsL gene for altered ribosomal protein S12.
Date: 31 Dec 92 01:41:05 GMT
Distribution: bionet
Checksum: 49029 5

LOCUS       SYNRPSLB     2247 bp ds-DNA             SYN       17-DEC-1992
DEFINITION  Synthetic rpsL gene for altered ribosomal protein S12.
ACCESSION   D10670
KEYWORDS    altered ribosomal protein S12.
SOURCE      Cloning vector DNA, clone pHSG683.
  ORGANISM  Cloning vector
            Artificial sequences; Cloning vehicles.
REFERENCE   1  (bases 1 to 2247)
  AUTHORS   Minowa,M. and Hashimoto-Gotoh,T.
  TITLE     Further improvement of Sms-enforcement cloning vectors with
            synthetic rpsL gene: Characterization of spontaneous elimination of
            streptomycin sensitivity gene on high copy number plasmids
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
COMMENT     Submitted (06-May-1992) to DDBJ by: Tamotsu Hashimoto-Gotoh
            Institute for Geriatrics and Neurological Diseases Dept. of
            Biochemistry and Molecular Genetics Kyoto Prefectural University of
            Medicine kawara-machi-Hirokoji Kajii-cho 465, Kamigyou-ku Kyoto 602
            Japan Phone:  075-251-5797 or 5850 Fax:    075-211-7093.
FEATURES             Location/Qualifiers
     misc_feature    1..375
                     /note="This feature applies to a gene which lacks a coding
                     region feature. "
                     /gene="rpsL"
     CDS             1..375
                     /product="altered ribosomal protein S12"
                     /gene="rpsL"
                     /codon_start=1
BASE COUNT      584 a    565 c    552 g    546 t
ORIGIN      
        1 atggcaacag ttaaccagct ggttcgaaag ccgcgagctc gtaaagtggc caaatctaac
       61 gttccggctc tcgaggcatg cccgtagaag cgtggcatat gcacacgcgt atacactact
      121 actccgaaga aaccgaattc agcgctgcgc aagctttgcc gcgtacgcct gaccaacggt
      181 ttcgaggtca cctcatatat aggtggtgaa ggacacaacc tgcaggaaca ctctgttatc
      241 ctgatcagag gcggccgcgt taaagatctg cccgggatcc ggtaccacac cgtccgcggc
      301 gctctagact gctccggagt aaaggaccgt cgacaggatc gatcgaaata cggtgtaaaa
      361 cgtccgaagg cctaatagaa gctgcttggc actgggccaa gctcgaattt ctgccattca
      421 tccgcttatt atcacttatt caggcgtagc accaggcgtt taagggcacc aataactgcc
      481 ttaaaaaaat tacgccccgc cctgccactc atcgcagtac tgttgtaatt cattaagcat
      541 tctgccgaca tggaagccat cacagacggc atgatgaacc tgaatcgcca gcggcatcag
      601 caccttgtcg ccttgcgtat aatatttgcc catggtgaaa acgggggcga agaagttgtc
      661 catattggcc acgtttaaat caaaactggt gaaactcacc cagggattgg ctgagacgaa
      721 aaacatattc tcaataaacc ctttagggaa ataggccagg ttttcaccgt aacacgccac
      781 atcttgcgaa tatatgtgta gaaactgccg gaaatcgtcg tggtattcac tccagagcga
      841 tgaaaacgtt tcagtttgct catggaaaac ggtgtaacaa gggtgaacac tatcccatat
      901 caccagctca ccgtctttca ttgccatacg aaattccgga tgagcattca tcaggcgggc
      961 aagaatgtga ataaaggccg gataaaactt gtgcttattt ttctttacgg tctttaaaaa
     1021 ggccgtaata tccagctgaa cggtctggtt ataggtacat tgagcaactg actgaaatgc
     1081 ctcaaaatgt tctttacgat gccattggga tatatcaacg gtggtatatc cagtgatttt
     1141 tttctccatt ttagcttcct tagctcctga aaatctcgat aactcaaaaa atacgcccgg
     1201 tagtgatctt atttcattat ggtgaaagtt ggaacctctt acgtgccgat caacgtctca
     1261 ttttcgccaa aagttggccc agggcttccc ggtatcaaca gggacaccag gatttattta
     1321 ttctgcgaag tgatcttccg ttcgacggag ttccactgag cgtcagaccc cgtagaaaag
     1381 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     1441 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     1501 aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag
     1561 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     1621 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     1681 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     1741 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagcattg agaaagcgcc
     1801 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     1861 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     1921 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     1981 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     2041 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     2101 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     2161 gaagaagcta ttctgaaatg agctgttgac aattaatcat cgaactagtt aactagtacg
     2221 caagttcacg taaaaagggt atcgacc
//