From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: P.sativum gene for phenylalanine ammonia-lyase, complete cds.
Date: 9 Jan 93 05:47:24 GMT
Distribution: bionet
Checksum: 25485 9

LOCUS       PEAPAL1      4415 bp ds-DNA             PLN       09-JAN-1993
DEFINITION  P.sativum gene for phenylalanine ammonia-lyase, complete cds.
ACCESSION   D10002
KEYWORDS    phenylalanine ammonia-lyase.
SOURCE      Pisum sativum (sub_species Midoriusui) epicotyl DNA.
  ORGANISM  Pisum sativum
            Eukaryota; Plantae; Embryobionta; Magnoliophyta; Magnoliopsida;
            Rosidae; Rosales; Fabaceaea.
REFERENCE   1  (bases 1 to 4415)
  AUTHORS   Yamada,T., Tanaka,Y., Sriprasertsak,P., Kato,H., Hashimoto,T.,
            Kawamata,S., Ichinose,Y., Kato,H., Shiraishi,T. and Oku,H.
  TITLE     Phenylalanine ammonia-lyase genes from Pisum sativum : Structure
            organ-specific expression and regulation by fungal elicitor and
            suppressor
  JOURNAL   Plant Cell Physiol. 33, 715-725 (1992)
  STANDARD  full automatic
COMMENT     Submitted (21-NOV-1991) to DDBJ by: Tetsuji Yamada Laboratory of
            Plant Pathology & Genetic Engineeing College of Agriculture Okayama
            University 1-1 Tsushima-naka Okayama 700 Japan Phone:  0862-52-1111
            x 722 Fax:    0862-54-0714.
FEATURES             Location/Qualifiers
     CAAT_signal     1331..1337
     TATA_signal     1361..1367
     prim_transcript 1395..>4232
     exon            1395..1933
                     /number=1
     intron          1934..2485
                     /number=1
     exon            2486..>4232
                     /number=2
     CDS             join(1509..1933,2486..4232)
                     /EC_number="4.3.1.5"
                     /gene="PAL1"
                     /product="phenylalanine ammonia-lyase"
                     /codon_start=1
                     /translation="METVAAAITKNNGYESFCVTNAKNNNMKVNSADPLNWGVAAEAM
                     KGSHLDEVKRMVEEYRKPVVRLGGETLTISQVAAIAAHDHGVKVELSESARAGVKASS
                     DWVMESMNKGTDSYGVTTGFGATSHRRTKQGGALQKELIRFLNAGIFGNGTESSHTLP
                     HTATRAAMLVRINTLLQGYSGIRFEILEAITKLINNNVTPCLLRGTITASGDLVPLSY
                     IAGLLTGRPNSKAHGTSGEILNAKEAFQSAEINDGFFELQPKEGLALVNGTAVGSGLA
                     SIVLFEANILAVLSEVLSAIFAEVMQGKPEFTDHLTHKLKHHPGQIEAAAIMEHILDG
                     SAYVKAAKKLHEMDPLQKPKQDRYALRTSPQWLGPLIEVIRFSTKSIEREINSVNDNP
                     LIDVSRNKALHGGNFQGTPIGVSMDNTRLALASIGKLLFAQFSELVNDFYNNGLPSNL
                     SASRNPSLDYGFKGSEIAMASYCSELQYLANPVTTHVQSAEQHNQDVNSLGLISSRKT
                     YEAIEILQLMSSTFLIALCQAVDLRHLEENLKNSVKNIVSQVAKRTLTTGVNGELHPS
                     RFCEKDLLRVVDREHVFAYIDDPCSATYPLMQKLRQVLVDHALVNGESEKNLNTSIFQ
                     KIATFEDELKTLLPKEVESTRAAYESGNPTVPNKINGCRSYPLYRFVRQELGTGLLTG
                     EKVISPGEECDKLFTAICQGKIIDPLLQCLGDWNGAPLPIS"
BASE COUNT     1415 a    724 c    794 g   1482 t
ORIGIN      
        1 aaaaatgtac ttgggacatt agagatcatt acgaaattaa ggaacttttt tactcgagat
       61 gtgatgtcta cctttttgac acacttgggg gtgctataat tacttggatt agaaaatgat
      121 cacgttcctg ttggatatgt gaagatatat ttcaagagct tattgtttat taaaattctg
      181 ataactttaa gcaaatatct ggagaaaaaa aaacataaag catctaaagt tggcggctgc
      241 aactacgcat cattaagtat taatgttgtt gaggttagcc cgaaggattg taaatttatt
      301 tattttttaa ttaaattttt atttagattt tagattaatt aaatataatt attattttta
      361 attatgtttt catttaatta gcgtgaagac cggagtataa ttctgtttct taatgagatc
      421 catgcggaga ctcatatcaa gaaaaataag gagtatgtac aagaaaaaat aaagcatgtt
      481 cttttgatat ttagttattt tttgacaaac ctaatttatt taaattatga taatatcttc
      541 ttttttatag gagaagtatg ataaagaact ccccatttat ctatcagaga atcctagatt
      601 acatgcaccg ccaccaagat atcctacttt ctagtgtgtc attcaagact tattatggtg
      661 tatcatacgg aaagaagaaa aataggagag tgtatggtgt tgaattattg accatacaaa
      721 acaaaatgag gttagatttg cgaaggataa aacctttgac aattaccaat gcgataaatc
      781 cctcacgaat atttattttg tgatgaattt ttgcacttgt gagagattta accctcacaa
      841 aagagtctta tagtgttatt tttatattaa tttgttaatt aatatgtagg aatgtagtat
      901 aattaaaaag gtgtagtcat ttatcctatt acttacaata ttgtgatttg agacactctt
      961 taagtaaatg atgattgata agtatagtag tataaaaatt tataaataat ataatgtatg
     1021 cattgggttg accgacattt agagttgaat ctaaagtcat ggtcatgcat ggttgcttcc
     1081 accatatttc ttgccaacta cctcgtgttt ctcttagtct attgccatcc acccatatgc
     1141 atctatctac caacccaaaa acaaagaaaa ccaaaaccct agattgccac gttacaaaat
     1201 cttaactgtt cattagtaag tgatgatcaa acggccataa ttaatattca acaaaccact
     1261 tttctttttt tctacttgtg caacttgtct ttcctcacct accaaactca catatcacac
     1321 caacacacat gcaatgcaca atactacatt tcaaagtctc tatataaagc ttaaccactc
     1381 ttccttcaca tctcaggaaa ctcataactc tttcattttc tttcataatc atttgagttt
     1441 ccattctctt gaaattcttg caaccaattc tcaacaaaga aacattagtt actttagtat
     1501 caattaaaat ggaaacagta gcagcagcca taacaaaaaa caacggttac gagtcatttt
     1561 gcgtgacaaa tgctaagaat aataacatga aagttaacag tgctgatcct ttgaattggg
     1621 gtgttgccgc cgaggcaatg aaagggagtc acttggatga ggtgaagcgt atggtggagg
     1681 agtacaggaa gccggtggtc cgccttggtg gcgagacact gacgatttct caggtggctg
     1741 ccattgccgc acatgatcat ggtgttaagg tggagttgtc agaatctgct agggctggcg
     1801 ttaaggcgag cagtgactgg gtgatggaga gtatgaacaa aggcacagac agttacggtg
     1861 ttactaccgg tttcggcgcc acctctcacc ggagaaccaa acaaggtggt gctttgcaga
     1921 aagaactcat caggtaatgt atactttttt tctttcttaa tttgcatata agtatctcct
     1981 tatagataaa tatcatggat ttattattaa agccaactat tttaattatt tagccacaaa
     2041 taaaaattct ttgcatgtat ataaattaaa tccattcaat atatatattt ctaaaaaatt
     2101 tccttattgt tatgagtaca ttaaatattt gataattttt ttgttgtttt ggtacagcta
     2161 tttgaaactt ttttaattag ttaatacata ttaaagtgtt tttttttcaa cgcagtactc
     2221 aaaaataaaa acaagcggta gggtattcta accactactg ataaatacca taaagtgcgt
     2281 tttattttgt ggaatgttat gaatttttca agtttactta aagcttcaac taattattaa
     2341 gtaacatttt tttatgagta aacatttgtt aacttacatt ttttagttta ttcaataaaa
     2401 aaactttttt tgtagtgtat gggtaacaaa aaatatattt ttaaattttt tttaattact
     2461 tactctactt aattcttgat ttcaggtttt tgaatgctgg aatatttgga aatggaactg
     2521 agtcaagcca tacactacca cacacagcaa caagagctgc catgcttgtg agaatcaaca
     2581 cacttctcca aggttattca ggaattagat ttgaaatctt ggaagctata accaaactca
     2641 ttaacaacaa cgtcacccca tgtttactcc gtggtacaat cacagcttcc ggagatttag
     2701 tccctctttc ttatattgct ggtttactaa cgggaagacc aaattcaaaa gctcatggga
     2761 cctctgggga aattcttaat gcaaaagaag cttttcagtc agctgaaatc aatgatggtt
     2821 tctttgaatt gcaaccaaaa gaaggtcttg cacttgttaa tggaactgct gttggttctg
     2881 gtttagcttc tattgttcta tttgaagcta acatattggc tgtcttgtct gaagtcctat
     2941 ccgctatttt tgctgaagtt atgcaaggga aacctgagtt tactgatcat ttgacacata
     3001 aattgaagca ccatcctggt caaattgagg ctgctgctat tatggaacac attttggatg
     3061 gaagtgctta tgtcaaagca gctaagaagt tgcatgagat ggatcctttg cagaaaccaa
     3121 aacaagatag atatgcactt agaacttcac cgcaatggct tggtcctctt attgaagtca
     3181 ttagattctc tactaagtca attgagaggg agatcaactc tgttaatgat aaccctttga
     3241 ttgatgtttc aagaaacaag gctttgcatg gtggaaactt tcaaggaaca cctattggtg
     3301 tatccatgga taatacacgt ttggctcttg cgtcaattgg taaactcttg tttgctcaat
     3361 tctctgaact cgtcaatgat ttttacaaca acgggttgcc ttcgaatctc tcagctagta
     3421 gaaatcccag cttggattat ggattcaagg gatccgaaat tgccatggct tcttattgtt
     3481 ctgagttaca atatcttgca aacccagtta caactcatgt tcaaagtgct gagcaacaca
     3541 accaagatgt gaactctttg ggtttgatat cttctaggaa aacatatgaa gccattgaga
     3601 tccttcaact catgtcttcc acattcttga ttgcactttg ccaagcagtt gacttaagac
     3661 atttggagga gaatttgaaa aactcagtta agaacattgt aagccaagtt gctaaaagga
     3721 cccttacaac aggtgtgaat ggagaacttc atccttcaag gttttgtgaa aaggacttgt
     3781 tgagagtggt tgatagggag catgtgtttg cctatattga tgatccttgt agtgctacat
     3841 atccattgat gcaaaaattg aggcaggtgt tagtggatca tgcattagta aatggtgaat
     3901 ctgagaagaa tttgaacaca tcaatcttcc aaaagattgc aacatttgag gatgagttaa
     3961 agaccctttt gccaaaagag gtagaaagta ctagggctgc atatgagagt ggaaatccaa
     4021 cagttccaaa caagataaat ggatgcagat cttatccact ttataggttt gtgagacaag
     4081 agttgggaac tggtttgcta acaggagaaa aggtcatttc accaggtgag gagtgtgata
     4141 agttgttcac agctatatgt caaggaaaga tcatcgatcc tcttcttcaa tgcttgggag
     4201 actggaacgg tgctcctctt ccaatttctt aactttaggt tattttaaga aaaattattt
     4261 gtatctatat atgcatgtat tgcaataatc actaggtttg tcatgctttt aacaattaat
     4321 atggaaatct ccatttcaat ttcttttaat gtcaatgtga aacttgtaat ttacttttat
     4381 aatagaactt catttgtttg acatagcttt taata
//