From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Saccharomyces cerevisiae dihydroorotic acid dehydrogenase (URA1)
Date: 29 Aug 92 10:02:25 GMT
Distribution: bionet
Checksum: 22674     7

LOCUS       YSCDHO       2884 bp ds-DNA             PLN       29-AUG-1992
DEFINITION  Saccharomyces cerevisiae dihydroorotic acid dehydrogenase (URA1)
            gene, complete cds.
ACCESSION   M83295
KEYWORDS    dihydroorotic acid dehydrogenase; pyrimidine synthesis;
            uracil auxotrophy.
SOURCE      Saccharomyces cerevisiae (strain FL100) (library: ATCC 28383) DNA.
  ORGANISM  Saccharomyces cerevisiae
            Eukaryota; Fungi; Eumycota; Hemiascomycetes; Endomycetales;
            Saccharomycetaceae.
REFERENCE   1  (bases 1 to 2884)
  AUTHORS   Roy,A.
  TITLE     Nucleotide sequence of the URA1 gene of Saccharomyces cerevisiae
  JOURNAL   Gene 118, 149-150 (1992)
  STANDARD  full staff_entry
FEATURES             Location/Qualifiers
     misc_feature    205..218
                     /note="UAS-URA1"
                     /gene="URA1"
     misc_feature    234..249
                     /note="UAS-URA2"
                     /gene="URA1"
     TATA_signal     281..286
                     /gene="URA1"
     5'UTR           355..416
                     /note="alternate transcription start"
                     /gene="URA1"
     5'UTR           358..416
                     /note="alternate transcription start"
                     /gene="URA1"
     5'UTR           362..416
                     /note="alternate transcription start"
                     /gene="URA1"
     5'UTR           366..416
                     /note="alternate transcription start"
                     /gene="URA1"
     5'UTR           374..416
                     /note="alternate transcription start"
                     /gene="URA1"
     5'UTR           379..416
                     /note="alternate transcription start"
                     /gene="URA1"
     5'UTR           381..416
                     /note="alternate transcription start"
                     /gene="URA1"
     5'UTR           384..416
                     /note="alternate transcription start"
                     /gene="URA1"
     CDS             417..1361
                     /EC_number="1.3.3.1"
                     /product="dihydroorotic acid dehydrogenase"
                     /gene="URA1"
                     /codon_start=1
                     /translation="MTASLTTKFLNNTYENPFMNASGVHCMTTQELDELANSKAGAFI
                     TKSATTLEREGNPEPRYISVPLGSINSMGLPNEGIDYYLSYVLNRQKNYPDAPAIFFS
                     VAGMSIDENLNLLRKIQDSEFNGITELNLSCPNVPGKPQVAYDFDLTKETLEKVFAFF
                     KKPLGVKLPPYFDFAHFDIMAKILNEFPLAYVNSINSIGNGLFIDVEKESVVVKPKNG
                     FGGIGGEYVKPTALANVRAFYTRLRPEIKVIGTGGIKSGKDAFEHLLCGASMLQIGTE
                     LQKEGVKIFERIEKELKDIMEAKGYTSIDQFRGKLNSI"
     CDS             complement(2029..2883)
                     /note="ORF 1"
                     /codon_start=1
                     /translation="SLIKLQVIIRPEKEEYIFNFDGTSPQVYGNLNAPEAITNSAILY
                     CLRCLVGEDIPLNQGCLKPLTIKIPAGSLLSPRSGAAVVGGNVLTSQRVTDVILKTFN
                     VMADSQGDCNNFTFGTGGNSGNKTDKQIKGFGYYETICGGSGAGADSWRGSGWNGSDA
                     VHTNMTNTRMTDTEVFERRYPVLLKEFSIRRGSGGKGKYTGGNGVVRDVQFRKAVTAS
                     ILSERRVIGPHGIKGGQDGSRGENLWVRHSTGALINVGGKNTIYAQPGDRFIIKTPGG
                     GGFGQYKD"
                     /partial
BASE COUNT      892 a    547 c    517 g    928 t
ORIGIN      chromosome XI
        1 ggatccaagt caatctggta tcttcccacc ctaaatagtg tattccccat gtaataagat
       61 cggacgataa acttcgaaac aattctagcc ttttttatta gatatttact ctactttaga
      121 tttggtgatt ttactaaatg ttatccttca gccactacag cctacttttt ctcactaata
      181 tgttcttgaa gccattttct tcaccgggct ttgccgaagg ttatttcacg atcttcggca
      241 ttcgccgaaa atttttcaag atgcccatca ccaaaaaata aaaaaagctt tatatatggc
      301 atggcatacg catcgggaac cggtattctt ctcttggatt acacagtttg gactactaga
      361 cgtttcaaat agatatacac aattactcaa aaaaaaattg aacttccgta ccaaacatga
      421 cagccagttt aactaccaag ttcttgaaca atacctatga aaacccattt atgaatgcat
      481 ccggtgttca ttgcatgact acacaagaat tagatgaatt agcaaactct aaagctggcg
      541 cattcattac aaagagtgct acaaccttag aaagagaagg taaccctgaa ccacgttaca
      601 tttctgtccc tctaggcagt atcaactcca tgggtttacc aaacgaaggt atcgactact
      661 atttgtccta tgtattaaac cgtcaaaaga attatcctga tgcacctgct attttcttct
      721 cagttgctgg tatgagcatt gatgaaaatt taaatttgtt gaggaaaatc caagatagcg
      781 aattcaacgg tattaccgag ttaaacttgt cttgtcctaa tgtgcctggg aaaccacaag
      841 ttgcttatga ctttgacttg acaaaggaaa ccttggaaaa ggtttttgcc tttttcaaaa
      901 aacctcttgg tgtcaagttg cctccttatt ttgattttgc ccattttgat atcatggcaa
      961 aaatattgaa cgagttccca ttagcttatg tcaactctat caatagtata ggaaatggtc
     1021 ttttcattga tgtggagaag gagagtgtag tagtgaagcc aaagaatggt ttcgggggta
     1081 ttggaggtga atatgttaag ccaaccgcgc tcgccaatgt tcgtgcattt tacactcgtt
     1141 tgagacctga aatcaaagtt atcggtacag gtggaattaa gtccggtaag gatgcatttg
     1201 aacatcttct atgtggtgcc tctatgctac agattggtac agaattacaa aaagagggcg
     1261 tcaagatttt tgaacgtatc gaaaaagaat taaaagacat aatggaagct aagggttata
     1321 catccataga tcagttccgt gggaagttga acagcattta attatcggaa tttgaagaac
     1381 tatgatggga caagttatgg aagagtgtta agattcgtat gaatatttta ttattttgaa
     1441 attgcataaa tcaaggatag ttggtaaatg ctaattcctt aatttttcgg tgagtgcatt
     1501 cgcaattggc atcatacatt agggttcaat gtgctttaat agatgatgat actaaatact
     1561 tatactttga aataacttaa tcaatgtaaa ttattgtctt gattcattta tacttaaatt
     1621 tttatatctc aacatatgat atatccttac attaagactt attcaagaaa catttttcaa
     1681 cgataattat tagtaggcta aaaatggcat aggaaccaga aaataatgtt gaagaaatga
     1741 agccatctgt ttaatatttg gggaaaaaag tgaagaaatt aacgaatatg tgcatatata
     1801 tatatatata tatatatata tatattgcac aggtgatttt gatgtagaat gtagcctttt
     1861 cgaaagagta atttggtttt atctacagag ttccgaaaca ttgttaagtg cctgttctta
     1921 tataattaaa ctgtactttc tgtaaaagtc aatttgtggc tggcgcatcc acggtatacc
     1981 aatggttccg tgtaattcga tattgactaa gaaccaaaaa gaaatgcttt aatccttgta
     2041 ttgtccaaat cccccaccac caggggtttt aatgatgaat cgatcaccgg gttgagcgta
     2101 aatcgtattt ttaccgccga cattgattaa tgcgcctgtg ctatgtctta cccataaatt
     2161 ctcacctcta ctaccgtctt gtcctccctt tataccatga gggccaatga cacgacgttc
     2221 tgataatata gatgctgtta cagccttccg aaattgaaca tcccttacta cgccatttcc
     2281 gccagtatat ttacctttgc ctcctgaacc tcttctgatc gagaactctt tcaacagaac
     2341 ggggtatctc ctttcaaata cctcagtatc tgtcattctt gtattggtca tattcgtgtg
     2401 tacagcgtct gagccattcc aaccacttcc tctccatgaa tctgcaccag cccccgagcc
     2461 gccacatatg gtttcataat atccaaagcc tttgatctgc ttatcggttt tgttaccaga
     2521 atttcctcca gttccaaatg taaagttgtt acagtcacct tgagaatctg ccatgacgtt
     2581 aaatgttttt aagattacat ctgtcactct ttgggaagtt aatacattgc ctccgacaac
     2641 agcagcaccg gatcgggggc tcaagaggga accagcagga atttttattg ttaagggttt
     2701 taaacaacct tgatttaaag gaatatcttc gcctactaga caccgtagac aataaagaat
     2761 cgcagaatta gtaatagcct ctggtgcatt taaattacca taaacttggg gagaagtgcc
     2821 atcgaaatta aaaatatact cctctttttc aggtcttatg ataacttgta gtttaattaa
     2881 gctt
//