From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: D.ambivalens lig gene for DNA ligase
Date: 21 Dec 92 20:40:44 GMT
Distribution: bionet
Checksum: 24903 8

LOCUS       DESLIG       3382 bp ds-DNA             BCT       18-NOV-1992
DEFINITION  D.ambivalens lig gene for DNA ligase
ACCESSION   X63438
KEYWORDS    DNA ligase; lig gene; thermoacidophile.
SOURCE      Desulfurolobus ambivalens DNA.
  ORGANISM  Desulfurolobus ambivalens
            Prokaryotae; Archaeobacteria; Thermoproteales; Desulfurococcaceae.
REFERENCE   1  (bases 1 to 3382)
  AUTHORS   Kletzin,A.
  TITLE     Molecular characterisation of a DNA ligase gene of the extremely
            thermophilic archaeon Desulfurolobus ambivalens shows close
            phylogenetic relationship to eukaryotic ligases
  JOURNAL   Nucleic Acids Res. 20, 5389-5396 (1992)
  STANDARD  full automatic
COMMENT     *source: strain=DSM 3772;
               From EMBL    entry DALIG;  dated 13-OCT-1992.
FEATURES             Location/Qualifiers
     misc_feature    878..883
                     /note="lig box A"
     misc_feature    906..909
                     /note="lig box B"
                     /evidence="EXPERIMENTAL"
     CDS             complement(250..771)
                     /note="ORF3"
                     /gene="ORF3"
                     /codon_start=1
                     /translation="MILGDRDLKYYLEKGWIVISPLTQDTIRENGVDLRVGGEIARFK
                     KTDEIYEDGKDPRSFYEIEKGDEFIIYPNEHVLLVTEEYVKLPNDVMAFVNLRSSFAR
                     LGLFVPPTIVDAGFEGQLTIEVLGSAFPVKIKRGTRFLHLIFARTLTPVENPYHGKYQ
                     GQQGVTLPKFKFR"
     CDS             908..2710
                     /evidence="EXPERIMENTAL"
                     /gene="lig"
                     /product="DNa ligase"
                     /codon_start=1
                     /translation="MEFKIIAEYFDRLEKISSRLQLTSLLADLFKKTDKNVIDKVVYI
                     IQGKLWPDFLGMPELGIGEKFLIRALSIATSVSDDEIEKMYKSVGDLGQVAFDIKQKQ
                     QSASILAFLGAQKASKPLTVEKVYDDLAKVATSTGEGSRDIKIRLLAGLLKDASPLEA
                     KYLVRFVDGRLRVGIGDATILDALAITFGGGQNFRPIVERAYNLRADLGNIAKILANG
                     GIEQLKNIKPQPGIPIRPMLAERLSDPAEMLSKVGNIALVDYKYDGERGQIHKAGDKI
                     FIFSRRLENITNQYPDVAEYISKYVKGNEFIVEGEIIPVDPETGEMRPFQELMHRKRK
                     SDIHEAIKEYPVNVFLFDLMYYEGEDYTVKPLSERRKKLESIVEDNDYVHIATHIITD
                     NVEKLKEFFYQAISEGAEGVMVKSLAPDAIYQAGSRGWLWIKFKRDYQSEMADTVDLV
                     MVGAFHGKGRKGGKYSSFLMAAYNPDKDVFETVCKVASGFTDAELDDLQKKIAELKRD
                     TPHPRVVSTMVPDVWLTPALVAEIIGAEITISPLHTCCKDQYAEGGLSIRFPRFIRWR
                     PDKSPEDATTNREILEMYKSQLKKIEEKPSDQSV"
     CDS             2961..>3382
                     /gene="ORF2"
                     /product="ORF2"
                     /codon_start=1
                     /translation="MGISYGVKIAIEDEKIELVQADHVFGAAQVVVTNSNGESVGYTG
                     DFKNPGKGTPILNTDVLIVDTTYGKPTFRRKFRDEVEVLFSDYVNDSLIYGPVRVYAY
                     YGKIQEAMKILRKNGISAPFIVDGKIKEVTDIAIKYGL"
BASE COUNT     1188 a    512 c    683 g    999 t
ORIGIN      
        1 aagcttaaga atcctgactt attctttgaa aaaattgaaa agcaaggtgg gaaagttcta
       61 tatactgata aagaaagaat agccgtagat gtcgatttct ttaataattt tgtaaagaaa
      121 ctttcagata ttcacacttc agatgatgta gaagcgcaaa aatacctcac taagcaagaa
      181 cataaattat tccaaaaatt gagacaattg ggaataatat acttcgataa taatactaag
      241 gcatggaagt tatcggaatt taaattttgg taaagttacg ccttgttgtc cttggtattt
      301 gccatgataa ggattctcta ctggtgttaa tgttctggcg aaaatgaggt gaagaaagcg
      361 agtccccctc tttatcttca cgggaaaagc agaacctagt acttctattg tgagttgtcc
      421 ttcaaagcct gcatctacga tagtcggtgg tacaaaaaga cccagcctgg caaaagaaga
      481 ccttaagttg acgaaagcca ttacgtcgtt cggcagttta acatactcct cagtaactaa
      541 cagtacatgt tcgttagggt aaatgatgaa ttcatctcct ttctctatct cataaaatga
      601 acgcgggtct tttccatctt catatatttc gtcagtcttc ttaaagcggg ctatctctcc
      661 tcctactctt aaatcgaccc cgttctctct tatagtatct tgagtaagag gagagattac
      721 tatccaaccc ttttctaggt agtattttaa atctctatct ccaagaatca taaatatcta
      781 ctatttttca tcttgcaaga atatatttta taaggaaaat ggcttcagag taactctgat
      841 acttcatcct tctggcttgc cggttttcat catcaaatat attatatcgt aattcaaact
      901 tttatttatg gagtttaaaa ttatagctga atactttgat agactagaaa agatctcttc
      961 taggcttcaa cttacatcat tacttgcaga tctttttaag aaaactgaca aaaatgttat
     1021 tgataaagtt gtatatataa ttcagggtaa actatggcca gacttcttag gaatgccaga
     1081 attaggaata ggtgaaaaat tccttataag agcattatca atagctacaa gcgtaagcga
     1141 tgatgaaata gaaaaaatgt ataaatcagt aggagatttg gggcaagtag cttttgatat
     1201 aaagcaaaaa cagcagtctg ccagtatttt agcgttttta ggagcgcaaa aagcttcaaa
     1261 gccgctaact gtagaaaaag tttatgatga tttagcaaaa gttgctacaa gcactggaga
     1321 aggtagtaga gatattaaaa tcagactatt agcaggatta cttaaggatg cttctccact
     1381 tgaggctaaa tatttagtta gatttgttga tggtaggtta agggttggaa taggagacgc
     1441 tacaatatta gacgcactag caataacttt cggcggcggg cagaacttta ggcctatagt
     1501 tgaaagagca tacaatttga gggccgattt aggtaatatt gcaaaaatat tagcaaatgg
     1561 cggaattgaa caactgaaga acattaaacc tcagccaggg attccaataa ggccaatgct
     1621 agctgaaaga ttatcagacc ctgcagagat gttaagtaag gtaggcaata ttgcattggt
     1681 cgattataag tatgatggag agagaggaca aattcataaa gcaggagata aaattttcat
     1741 attctctaga agattggaaa atataacaaa tcaatatcct gacgtagcag agtatatttc
     1801 aaaatacgtt aaaggtaatg agttcatagt tgagggagaa ataataccag tagatccaga
     1861 aactggtgaa atgagacctt tccaagaatt gatgcacagg aaaaggaagt ccgatattca
     1921 cgaggctata aaagagtatc cggttaacgt attcttattt gatttaatgt actatgaagg
     1981 agaagattat acagtaaagc cgttatcaga aagacgtaag aagttagaat caatagttga
     2041 agataacgat tatgtacata tagccactca tattataact gataatgtag aaaaactaaa
     2101 ggaattcttc tatcaagcaa tctctgaagg cgcagaaggc gtaatggtaa aatctctggc
     2161 accagatgca atatatcaag caggatcaag aggttggctt tggataaagt ttaagagaga
     2221 ttaccagagc gaaatggcag atactgtaga cctggtaatg gtaggtgctt ttcacggtaa
     2281 aggaaggaaa ggaggcaaat atagttcctt cttaatggct gcatataatc cagacaagga
     2341 cgttttcgag actgtatgta aagttgcttc tggttttact gatgcagaac ttgacgattt
     2401 acagaagaaa attgccgagt taaaaagaga cacacctcat ccaagagttg tatcaaccat
     2461 ggttccagat gtttggttaa caccagcact tgttgccgaa ataattggag cagaaataac
     2521 tatatctcct ctacatactt gttgcaaaga tcagtatgca gaaggtggtt tatcaataag
     2581 attcccaaga tttataaggt ggagaccaga taagagtcca gaagatgcaa ctactaatcg
     2641 tgaaattctt gagatgtata agagccaatt aaagaagatt gaagaaaagc cgtcagatca
     2701 gagtgtataa gtttttataa aataactcat agttatgttt tgtgttcttc gatattaaga
     2761 agtctggtgc aatactttta ggtaacaact ttacagtaga tggacattat gagcgaaatt
     2821 ttagggttgt aactcatttt catgctgacc atattttgca attaaataag agcgtatcat
     2881 cttgtattgg gattatatca actcctccaa ctcttgaagt tctctcagtt ttagggtatg
     2941 atatacctaa gaaaaaagca atgggaataa gttatggtgt aaaaattgca atagaagatg
     3001 agaaaataga actagttcag gcagatcatg tatttggagc cgctcaagta gttgtaacaa
     3061 attccaatgg cgaaagtgta ggttatactg gagattttaa gaatccgggt aaaggtactc
     3121 ctatacttaa tactgacgta ttaattgttg atactaccta cggaaagccg acatttagaa
     3181 gaaaatttag agatgaagtt gaagtcttat tttcagatta cgtaaacgat tcattaattt
     3241 atggaccagt aagagtttat gcttactatg gaaagattca agaggcaatg aaaatactga
     3301 ggaaaaatgg aatttcggcc ccttttatag tagatggtaa aataaaagaa gttacagata
     3361 ttgcaataaa gtatggtcta ga
//