From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Streptomyces thermoviolaceus alpha-amylase (amy) gene, complete
Date: 29 Dec 92 19:51:46 GMT
Distribution: bionet
Checksum: 41415 5

LOCUS       STMAMY       1712 bp ds-DNA             BCT       09-DEC-1992
DEFINITION  Streptomyces thermoviolaceus alpha-amylase (amy) gene, complete
            cds.
ACCESSION   M34957
KEYWORDS    alpha-amylase.
SOURCE      Streptomyces thermoviolaceus (strain CUB74) DNA.
  ORGANISM  Streptomyces thermoviolaceus
            Prokaryotae; Firmicutes; Streptomycetaceae.
REFERENCE   1  (bases 1 to 1712)
  AUTHORS   Bahri,S.M. and Ward,J.M.
  TITLE     Nucleotide sequence analysis of an alpha-amylase gene isolated from
            Streptomyces thermoviolaceus CUB74
  JOURNAL   Gene (1990) In press
  STANDARD  full automatic
COMMENT     Draft entry and printed sequence for [1] kindly submitted by
            S.Bahri 08-JUN-1990.
FEATURES             Location/Qualifiers
     mRNA            124..(1621.1646)
                     /gene="amy"
     sig_peptide     215..277
                     /gene="amy"
                     /codon_start=1
     mat_peptide     278..1591
                     /product="alpha-amylase"
                     /gene="amy"
                     /codon_start=1
     CDS             215..1597
                     /EC_number="3.2.1.1"
                     /gene="amy"
                     /note="putative"
                     /product="alpha-amylase"
                     /codon_start=1
                     /translation="MASRTLSGALALAAAATAVLAAPATVAHRSPPGTKDVTAVLFEW
                     DYVSVAKECTSTLGPAGYGYVQVSPPAEHIQGSQWWTSYQPVSYKIAGRLGDRAAFRS
                     MVNTCHAAGVKVVVDTVINHMSAGSGTGTGGSSYTKYDYPGLYSAPDFDDCTAEITDY
                     QDRWNVQHCELVGLADLDTGEEYVRQTIAGYMNDLLSLGVDGFRIDAATHIPAEDLAN
                     IKSRLSNPNAYWKQEVIYGAGEPPKPGEYTGTGDVQEFRYAYDLKRVFTQEHLAYLKN
                     YGEDWGYLSSTTAGVFVDNHDTERNGSTLNYKNDATYTLANVFMLAWPYGAPDINSGY
                     EWSDPDARPPDGGHVDACWQNGWKCQHKWPEIASMVAFRNATRGEPVTDWWDDGADAI
                     AFGRGSKGFVAINHESATVQRTYQTSLPAGTYCDVQSNTTVTVDSAGRFTAALGPDTA
                     LALHNGRTSC"
BASE COUNT      303 a    662 c    520 g    227 t
ORIGIN      
        1 gatcccgcgg acggatcgtg cccgcacacc ccctgccggc ctgcactgca ccatgaaccc
       61 ttcatggtct ctgacaggtt catgtccaga gtattgaccg cccgcaacgg cgctcgtacg
      121 gtcggctgca cgtcgcagcg ctgaaagctt gcagcaagtt cttccatccc cttgtccgtc
      181 cgacgcgacc ctccgacgga gaaagaaccc ccacatggcc agcagaacgc tctcgggcgc
      241 gctcgccctg gcggccgccg cgacggccgt cctcgccgct cccgccaccg tcgcccaccg
      301 ctccccgccc ggcaccaagg acgtcaccgc cgtcctcttc gagtgggact acgtctccgt
      361 ggccaaggag tgcaccagca ccctcggccc ggccggctac ggctacgtgc aggtctcccc
      421 gcccgccgag cacatccagg gctcccagtg gtggacgtcg taccagccgg tgagctacaa
      481 gatcgccggc cggctcggcg accgtgccgc cttccgatcc atggtgaaca cctgccacgc
      541 cgccggggtg aaggtggtcg tcgacacggt gatcaaccac atgtcggccg gcagcggcac
      601 cggcaccgga ggctcgtcgt acacgaagta cgactacccg gggctgtact cggccccgga
      661 cttcgacgac tgcaccgccg agatcaccga ctaccaggac cgctggaacg tccagcactg
      721 cgaactggtg ggcctcgccg acctcgacac cggtgaggag tacgtgcgac agacgatcgc
      781 cggctacatg aacgacctgc tctccctcgg cgtcgacggc ttccgcatcg acgcggccac
      841 gcacatcccc gccgaggacc tcgcgaacat caagtcccgc ctgagcaacc cgaacgccta
      901 ctggaagcag gaggtcatct acggcgccgg cgaacctccc aagcccggcg agtacaccgg
      961 caccggcgac gtccaggagt tccgctacgc ctacgacctc aagcgggtct tcacccagga
     1021 gcacctcgcc tacctgaaga actacggcga ggactggggc tacctgagca gcacgacggc
     1081 cggggtcttc gtcgacaacc acgacaccga gcgcaacggc tccacgctga actacaagaa
     1141 cgacgccacc tacaccctgg ccaacgtctt catgctggct tggccctacg gcgcccccga
     1201 catcaattcc ggctacgagt ggtccgaccc ggacgcccgg ccgcccgacg gcggccacgt
     1261 cgacgcctgc tggcagaacg gctggaagtg ccagcacaag tggcccgaga tcgcctccat
     1321 ggtcgccttc cgcaacgcca cccgcggcga gccggtcacc gactggtggg acgacggcgc
     1381 ggacgccatc gccttcggcc ggggcagcaa gggcttcgtg gccatcaacc acgagtccgc
     1441 caccgtccag cgcacctacc agacctccct gcccgccggc acctactgcg acgtgcagag
     1501 caacaccacg gtgacggtgg actccgccgg acggttcacc gccgcgctcg gcccggacac
     1561 ggcactggcc ctgcacaacg gcaggacgag ctgctgaccg tccgcggcgg attcacccgg
     1621 acgggcccac ggtgcgtgcg ccgtgggccc gagccgtgcc gtggcgtcgc ggcgtggccc
     1681 gagggcggac cgagcggagc aatcgggcat gc
//