From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human nidogen mRNA, complete cds.
Date: 5 Nov 92 23:11:45 GMT
Distribution: bionet
Checksum: 27370 10

LOCUS       HUMNID       4898 bp ss-mRNA            PRI       15-MAR-1990
DEFINITION  Human nidogen mRNA, complete cds.
ACCESSION   M30269
KEYWORDS    nidogen.
SOURCE      Human placenta and skin fibroblast, cDNA to mRNA, clones
            cHFN-[5,7,8,12,16,26].
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 4898)
  AUTHORS   Nagayoshi,T., Sanborn,D., Hickok,N.J., Olsen,D.R., Fazio,M.J.,
            Chu,M.-L., Knowlton,R., Mann,K., Deutzmann,R., Timpl,R. and
            Uitto,J.
  TITLE     Human nidogen: Complete amino acid sequence and structural domains
            deduced from cDNAs, and evidence for polymorphism of the gene
  JOURNAL   DNA 8, 581-594 (1989)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     sig_peptide     91..174
                     /gene="NID"
                     /codon_start=1
     mat_peptide     175..3831
                     /product="nidogen"
                     /gene="NID"
                     /codon_start=1
     CDS             91..3834
                     /gene="NID"
                     /product="nidogen"
                     /codon_start=1
                     /translation="MLASSSRIRAAWTRALLLPLLLAGPVGCLSRQELFPFGPGQGDL
                     ELEDGDDFVSPALELSGALRFYDRSDIDAVYVTTNGIIATSEPPAKESHPGLFPPTFG
                     AVAPFLADLDTTDGLGKVYYREDLSPSITQRAAECVHRGFPEISFQPSSAVVVTWESV
                     APYQGPSRDPDQKGKRNTFQAVLASSDSSSYAIFLYPEDGLQFHTTFSKKENNQVPAV
                     VAFSQGSVGFLWKSNGAYNIFANDRESIENLAKSSNSGQQGVWVFEIGSPATTNGVVP
                     ADVILGTEDGAEYDDEDEDYDLATTRLGLEDVGTTPFSYKALRRGGADTYSVPSVLSP
                     RRAATERPLGPPTERTRSFQLAVETFHQQHPQVIDVDEVEETGVVFSYNTDSRQTCAN
                     NRHQCSVHAECRDYATGFCCSCVAGYTGNGRQCVAEGSPQRVNGKVKGRIFVGSSQVP
                     IVFENTDLHSYVVMNHGRSYTAISTIPETVGYSLLPLAPVGGIIGWMFAVEQDGFKNG
                     FSITGGEFTRQAEVTFVGHPGNLVIKQRFSGIDEHGHLTIDTELEGRVPQIPFGSSVH
                     IEPYTELYHYSTSVITSSSTREYTVTEPERDGASPSRIYTYQWRQTITFQECVHDDSR
                     PALPSTQQLSVDSVFVLYNQEEKILRYAFSNSIGPVREGSPDALQNPCYIGTHGCDTN
                     AACRPGPRTQFTCECSIGFRGDGRTCYDIDECSEQPSVCGSHTICNNHPGTFRCECVE
                     GYQFSDEGTCVAVVDQRPINYCETGLHNCDIPQRAQCIYTGGSSYTCSCLPGFSGDGQ
                     ACQDVDECQPSRCHPDAFCYNTPGSFTCQCKPGYQGDGFRCVPGEVEKTRCQHEREHI
                     LGAAGATDPQRPIPPGLFVPECDAHGHYAPTQCHGSTGYCWCVDRDGREVEGTRTRPG
                     MTPPCLSTVAPPIHQGPAVPTAVIPLPPGTHLLFAQTGKIERLPLEGNTMRKTEAKAF
                     LHVPAKVIIGLAFDCVDKMVYWTDITEPSIGRASLHGGEPTTIIRQDLGSPEGIAVDH
                     LGRNIFWTDSNLDRIEVAKLDGTQRRVLFETDLVNPRGIVTDSVRGNLYWTDWNRDNP
                     KIETSYMDGTNRRILVQDDLGLPNGLHFDAFSSQLCWVDAGTNRAECLNPSQPSRRKA
                     LEGLQYPFAVTSYGKNLYFTDWKMNSVVALDLAISKETDAFQPHKQTRLYGITTALSQ
                     CPQGHNYCSVNNGGCTHLCLATPGSRTCRCPDNTLGVDCIERK"
BASE COUNT     1096 a   1417 c   1293 g   1092 t
ORIGIN      
        1 gaattccggc tgccaggggc gtccggttac atccccgcct tcctctgtcc tggccgcggg
       61 accgggtttg cgggaccgca gttcgggaac atgttggcct cgagcagccg gatccgggct
      121 gcgtggacgc gggcgctgct gctgccgctg ctgctggcgg ggcctgtggg ctgcctgagc
      181 cgccaggagc tctttccctt cggccccgga cagggggacc tggagctgga ggacggggat
      241 gacttcgtct ctcctgccct ggagctgagt ggggcgctcc gcttctacga cagatccgac
      301 atcgacgcag tctacgtcac cacaaatggc atcattgcta cgagtgaacc cccggccaaa
      361 gaatcccatc ccgggctctt cccaccaaca ttcggtgcag tcgccccttt cctggcggac
      421 ttggacacga ccgatggcct ggggaaggtt tattatcgag aagacttatc cccctccatc
      481 actcagcgag cagcagagtg tgtccacaga gggttcccgg agatctcttt ccagcctagt
      541 agcgcggtgg ttgtcacttg ggaatccgtg gccccctacc aagggcccag cagggaccca
      601 gaccagaaag gcaagagaaa cacgttccag gctgttctag cctcctctga ttccagctcc
      661 tatgccattt tcctttatcc tgaggatggt ctgcagttcc atacgacatt ctcaaagaag
      721 gaaaacaacc aagttcctgc cgtggttgca ttcagtcaag gttcagtggg attcttatgg
      781 aagagcaacg gagcttataa catatttgct aatgacaggg aatcaattga aaatttggcc
      841 aagagtagta actctgggca gcagggtgtc tgggtgtttg agattgggag tccagccacc
      901 accaatggcg tggtgcctgc agacgtgatc ctcggaactg aagatggggc agagtatgat
      961 gatgaggatg aagattatga cctggcgacc actcgtctgg gcctggagga tgtgggcacc
     1021 acgcccttct cctacaaggc tctgagaagg ggaggtgctg acacatacag tgtgcccagc
     1081 gtcctctccc cgcgccgggc agctaccgaa aggccccttg gacctcccac agagagaacc
     1141 aggtctttcc agttggcagt ggagactttt caccagcagc accctcaggt catagatgtg
     1201 gatgaagttg aggaaacagg agttgttttc agctataaca cggattcccg ccagacgtgt
     1261 gctaacaaca gacaccagtg ctcggtgcac gcagagtgca gggactacgc cacgggcttc
     1321 tgctgcagct gtgtcgctgg ctatacgggc aatggcaggc aatgtgttgc agaaggttcc
     1381 ccccagcgag tcaatggcaa ggtgaaagga aggatctttg tggggagcag ccaggtcccc
     1441 attgtctttg agaacactga cctccactct tacgtagtaa tgaaccacgg gcgctcctac
     1501 acagccatca gcaccattcc cgagaccgtt ggatattctc tgcttccact ggccccagtt
     1561 ggaggcatca ttggatggat gtttgcagtg gagcaggacg gattcaagaa tgggttcagc
     1621 atcaccgggg gtgagttcac tcgccaggct gaggtgacct tcgtggggca cccgggcaat
     1681 ctggtcatta agcagcggtt cagcggcatc gatgagcatg ggcacctgac catcgacacg
     1741 gagctggagg gccgcgtgcc gcagattccg ttcggctcct ccgtgcacat tgagccctac
     1801 acggagctgt accactactc cacctcagtg atcacttcct cctccacccg ggagtacacg
     1861 gtgactgagc ccgagcgaga tggggcatct ccttcacgca tctacactta ccagtggcgc
     1921 cagaccatca ccttccagga atgcgtccac gatgactccc ggccagccct gcccagcacc
     1981 cagcagctct cggtggacag cgtgttcgtc ctgtacaacc aggaggagaa gatcttgcgc
     2041 tacgctttca gcaactccat tgggcctgtg agggaaggct cccctgatgc tcttcagaat
     2101 ccctgctaca tcggcactca tgggtgtgac accaacgcgg cctgtcgccc tggtcccagg
     2161 acacagttca cctgcgagtg ctccatcggc ttccgaggag acgggcgaac ctgctatgat
     2221 attgatgaat gttcagaaca accctcagtg tgtgggagcc acacaatctg caataatcac
     2281 ccaggaacct tccgctgcga gtgtgtggag ggctaccagt tttcagatga gggaacgtgt
     2341 gtggctgtcg tggaccagcg ccccatcaac tactgtgaaa ctggccttca taactgcgac
     2401 ataccccagc gggcccagtg tatctacaca ggaggctcct cctacacctg ttcctgcttg
     2461 ccaggctttt ctggggatgg ccaagcctgc caagatgtag atgaatgcca gccaagccga
     2521 tgtcaccctg acgccttctg ctacaacact ccaggctctt tcacgtgcca gtgcaaacct
     2581 ggttatcagg gagacggctt ccgttgcgtg cccggagagg tggagaaaac ccggtgccag
     2641 cacgagcgag aacacattct cggggcagcg ggggcgacag acccacagcg acccattcct
     2701 ccggggctgt tcgttcctga gtgcgatgcg cacgggcact acgcgcccac ccagtgccac
     2761 ggcagcaccg gctactgctg gtgcgtggat cgcgacggcc gcgaggtgga gggcaccagg
     2821 accaggcccg ggatgacgcc cccgtgtctg agtacagtgg ctcccccgat tcaccaagga
     2881 cctgcggtgc ctaccgccgt gatccccttg cctcctggga cccatttact ctttgcccag
     2941 actgggaaga ttgagcgcct gcccctggag ggaaatacca tgaggaagac agaagcaaag
     3001 gcgttccttc atgtcccggc taaagtcatc attggactgg cctttgactg cgtggacaag
     3061 atggtttact ggacggacat cactgagcct tccattggga gagctagtct acatggtgga
     3121 gagccaacca ccatcattag acaagatctt ggaagtccag aaggtatcgc tgttgatcac
     3181 cttggccgca acatcttctg gacagactct aacctggatc gaatagaagt ggcgaagctg
     3241 gacggcacgc agcgccgggt gctctttgag actgacctgg tgaatcccag aggcattgta
     3301 acggattccg tgagagggaa cctttactgg acagactgga acagagataa ccccaagatt
     3361 gaaacttcct acatggacgg cacgaaccgg aggatccttg tgcaggatga cctgggcttg
     3421 cccaatggac tgcacttcga tgcgttctca tctcagctct gctgggtgga tgcaggcacc
     3481 aatcgggcgg aatgcctgaa ccccagtcag cccagcagac gcaaggctct cgaagggctc
     3541 cagtatcctt ttgctgtgac gagctacggg aagaatctgt atttcacaga ctggaagatg
     3601 aattccgtgg ttgctctcga tcttgcaatt tccaaggaga cggatgcttt ccaaccccac
     3661 aagcagaccc ggctgtatgg catcaccacg gccctgtctc agtgtccgca aggccataac
     3721 tactgctcag tgaacaatgg cggctgcacc cacctatgct tggccacccc agggagcagg
     3781 acctgccgtt gccctgacaa caccttggga gttgactgta tcgaacggaa atgaagacaa
     3841 gagtgcctta tttcctttcc aagtatttca cagcaacact ctacttgaag caacttggtc
     3901 cagattgaaa agtgtcctct ggctgagtgg ccactaggcc cagacccagc ccagcctgag
     3961 ccccaacaac aacttttccc tcactgttcc ccaaaacatg caccctggac ttctctaata
     4021 gaaaagtctc cacccctaca caaggacaga accctccacc cctaccccca accctcagac
     4081 agacttatac acccctgagt gaggattaca tgcccatccc agtgtcctag gaccttttcc
     4141 caatactagc cccccagtgg tgaacagaac ctcccaaatt tgagttgcac ccttccctgt
     4201 ggccttatga gctcagcctc gctttgaggt acccaccgtc ctgtcagctc cttgacctat
     4261 gagctggggc ctgactagga aaagttggga gttaaggagg aaattagcat tccttaatgt
     4321 tttgttttgg tgctctgaat ttcttcttta ttatagtcct atagttttac tcctcagttc
     4381 ctcaccatca tcatcttgtc taagaccccc attataatat tcatgcgctg ctttttcatc
     4441 aaaacctacc ctgtcctaga gatctatggg catttggtgg atgataatga gcagcccctc
     4501 ccagatagaa tgtcaatatt tgagcagtag gatattggca tttgttagtt aaaggcttaa
     4561 atcaaaagaa tgtccaatgg taggaatttc aaggtgtagg tcagatattt gagaataggg
     4621 gatttttttg atgtgcctta aattatacca aagattacta attattcctc tttgcccaaa
     4681 atacttgcat ccaaggttct agtctctgtt gctgtgctgg tctttagccc cactgctggc
     4741 actgatgtcc ctcctttttc acggagacct atctgaggta caggatgggg ctggcaccag
     4801 atgatgtccc accacagtcc ctcacctccg gcctccacat gacagaacca atttacactc
     4861 aaccatgacc tcacccctcc ttggtttctc cctccccg
//