From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Arabidopsis thaliana alpha-2 tubulin (TUA2) gene, exon and 5' end.
Date: 25 Jul 92 10:02:27 GMT
Distribution: bionet
Checksum: 29540     7

LOCUS       ATHTUBA2A    3447 bp ds-DNA             PLN       25-JUL-1992
DEFINITION  Arabidopsis thaliana alpha-2 tubulin (TUA2) gene, exon and 5' end.
ACCESSION   M84696
KEYWORDS    alpha-2 tubulin.
SOURCE      Arabidopsis thaliana (strain Columbia) (library: cosmid pOCA18;
            Olszewski) DNA.
  ORGANISM  Arabidopsis thaliana
            Eukaryota; Plantae; Embryobionta; Magnoliophyta; Magnoliopsida;
            Dilleniidae; Capparales; Brassicaceae.
REFERENCE   1  (sites)
  AUTHORS   Kopczak,S.D., Haas,N.A., Hussey,P.J., Silflow,C.D. and Snustad,D.P.
  TITLE     The small genome of Arabidopsis contains at least six expressed
            alpha-tubulin genes
  JOURNAL   Plant Cell 4, 539-547 (1992)
  STANDARD  full staff_review
REFERENCE   2  (sites)
  AUTHORS   Snustad,D.P., Haas,N.A., Kopczak,S.D. and Silflow,C.D.
  TITLE     The small genome of Arabidopsis contains at least nine expressed
            beta-tubulin genes
  JOURNAL   Plant Cell 4, 549-556 (1992)
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     CDS             join(1054..1146,1609..1843,1937..2307,2393..3046)
                     /product="apha-2 tubulin"
                     /gene="TUA2"
                     /codon_start=1
                     /translation="MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMPSDKTVGG
                     GDDAFNTFFSETGAGKHVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLISGKEDAANN
                     FARGHYTIGKEIVDLCLDRIRKLADNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVD
                     YGKKSKLGFTVYPSPQVSTSVVEPYNSVLSTHSLLEHTDVSILLDNEAIYDICRRSLS
                     IERPTYTNLNRLVSQVISSLTASLRFDGALNVDVTEFQTNLVPYPRIHFMLSSYAPVI
                     SAEKAFHEQLSVAEITNSAFEPASMMAKCDPRHGKYMACCLMYRGDVVPKDVNAAVGT
                     IKTKRTIQFVDWCPTGFKCGINYQPPTVVPGGDLAKVQRAVCMISNSTSVAEVFSRID
                     HKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKDYEEVGAEGGDDEDDEGEEY
                     "
     exon            1054..1146
                     /number
                     /gene="TUA2"
     exon            1054..1146
                     /number
                     /gene="TUA2"
     intron          1147..1608
                     /number=1
                     /gene="TUA2"
     exon            1609..1843
                     /number=2
                     /gene="TUA2"
     intron          1844..1936
                     /number=2
                     /gene="TUA2"
     exon            1937..2307
                     /number=3
                     /gene="TUA2"
     intron          2308..2392
                     /number=3
                     /gene="TUA2"
BASE COUNT      948 a    696 c    714 g   1089 t
ORIGIN
        1 cctttcatga atgcaacact ggaaaatgac aataacatat atgaatgtag taattacctg
       61 tcctcctgtt tcagtctaga cttagccata tgccaaaact cagagccttg aacacgaaca
      121 actccaccct ataaacaatc ataaaacgat ttacacaaag aggagcttat catttcaaac
      181 aacaagaaca tttaatatta tcatttcacc atgattttac cagaaatttc ttagagatta
      241 cagtgaatta cagtaatttc taaacttctc agtgattacc ctcactctca tttcgattgg
      301 attcttacac agatttaaag ggaacaaaaa ttatatgaga taaagataga gatagatagg
      361 gtgtgtgcct tgcgagatgg aagatcatcg gaaaagaatc gaggaaggcc accgcgagat
      421 tgattccgga gattcaaatc cgacggagga gacgaagata cagtagcaaa cgatttgaag
      481 ctagaagtcg gaagccatga acgaacgaga ctcggcaaat cggaaaacag tggccgtgaa
      541 gccgccgcta ggcctctcat catttactca aagctttgaa tttttggtta ccatttcgaa
      601 gattttggtt aatactagtt tcttatcgtt tcggtttact ttaaatattg gtctgatccg
      661 gttcaattct aattaaattc cggttcattt ccacgcaatt caaggaattt tgctatttca
      721 aaaataaaga gaaacggtca gaaagcgatt gccaaacaga atctaaggaa tcaaaattca
      781 aaattagaaa atacgcaact cacgaggata tcaacaaatc gtggttttaa cgacaaccca
      841 attacatcac tacacgtgta tcatacgtgc cgttacatac agatggatac tttcgcttat
      901 aaatatagca tcaaacagct ctgtaaaacc tagaaatcga aaaatcagat ctagatctaa
      961 aagaagagcg tcttcataaa cgcccttctt cttcttcgtc ttcttccctg caaatctagt
     1021 ttctagatct tttctttctt ccgcgaaacg aaaatgagag agtgcatttc gatccacatt
     1081 ggtcaggccg gtatccaggt cggaaatgct tgctgggagc tttactgtct tgaacatggc
     1141 attcaggttc gtctcttcct cttctatctt gatctgtgtt tttcttcata tttttcgtat
     1201 tttaaaagat caaatctgag atatttttgc gcagcattga cctcgtagat ctattttacg
     1261 taatcgttta gatctgtgtt tgatttcgac gtcttatgaa atttctgtgg ttgatgcgaa
     1321 atccgtttct catttctaaa ttattcgatg aacctggata gatctgtgtt tgaattggat
     1381 ttcatgtcag attgcttttg ctgatgccat atccgtattt gttttataat attgggtgaa
     1441 ggaaaacttt gctttatttg cttaaagaga tggcgattga tatttgcgat atagttgaag
     1501 catctcgttg tgattttctc ttttcatcgt tgcttctata tgtgtagatc ttttcgtttt
     1561 atatgtctct ttgtattgat gtgttgtctt ggatgtggaa tgttacagcc tgatggccag
     1621 atgccgagtg acaagactgt tggcggaggt gacgatgctt tcaacacctt cttcagtgag
     1681 accggtgcag ggaagcacgt cccacgtgct gtctttgttg atcttgagcc aactgtgatc
     1741 gatgaggtca ggactggtac ttaccgtcag cttttccacc ctgagcaact catcagcggt
     1801 aaagaagacg cagctaacaa tttcgcccgt gggcattaca ccagtaagtt actccttccc
     1861 ttgtttcttc atcatcaacc atatcttgtt gaatttactg ttcattcact gatttacttt
     1921 tgaaatctta tttcagttgg gaaagagatt gttgatctgt gcttggaccg tatcagaaag
     1981 ctcgctgata actgtactgg tctccaagga ttcctcgtct tcaacgctgt tggtggaggg
     2041 actggatctg gtcttggatc tctcctcctt gagagacttt ctgttgacta cgggaaaaag
     2101 tccaagttgg gtttcacagt ttacccatct ccacaggtct ccacctctgt tgttgagcct
     2161 tacaacagtg tcctctccac tcactcactc ttggaacaca ctgatgtctc tatcctcctc
     2221 gacaatgaag ctatctatga catctgcaga cgttccctaa gcattgagag acccacctac
     2281 accaacctca accgtctcgt ctctcaggta tagagccagc ttcaatcatt tacatcagtc
     2341 agtctttttg ctgtttcgtt tcttatgttt aactctctct tgatggtttc aggttatctc
     2401 atccttgact gcttctctga ggttcgatgg tgccttgaat gttgatgtga ctgagttcca
     2461 aaccaacttg gtcccatacc caagaatcca cttcatgctt tcctcctatg ccccagtcat
     2521 ctccgcagag aaggccttcc atgagcagct ctcggttgct gagatcacaa acagtgcttt
     2581 tgagccagca tccatgatgg caaagtgtga ccctcgtcac ggaaagtaca tggcttgctg
     2641 tttgatgtac cgtggtgatg tcgtccccaa ggatgtgaac gcagctgttg gcaccatcaa
     2701 gaccaagcgt actattcagt ttgtggactg gtgtcctact ggattcaagt gtggaatcaa
     2761 ctaccagcca ccaactgttg ttccaggagg tgatcttgct aaagtccaga gagctgtgtg
     2821 tatgatctca aactccacca gtgttgctga ggtgttctcc cgtattgatc acaagtttga
     2881 tcttatgtac gcaaaacgtg ctttcgttca ctggtatgtg ggtgagggta tggaagaagg
     2941 agaattttca gaggctcgtg aggatcttgc agcattggag aaggattacg aagaggttgg
     3001 tgctgaaggt ggtgacgatg aggatgatga aggagaggag tactaagaag gttgtcggtt
     3061 ttatgtggga tttctattat ctcgtgtttg tgtgaatagg ctcgaaactc ttacgagtcg
     3121 tacttgtgtt ttcaaaaacc atatttctat ctcttgcttg tggcggtagc catttgttct
     3181 agttcgtttg tttctgataa tattttcatc tccaagaact gaatttaagc tgggtttggt
     3241 ttaaatcgat tgtccaaaat ctaaacaaat agaaacgtca aattgttatt tgagtggtga
     3301 aagattgaaa tttttggaac agagaagcaa tagagaagca acgattcttt ttacaaccgt
     3361 aaggaaaata aataaaagga aaggctgtaa ataaaaatat atacttagag caaatatatg
     3421 ctctacacta gttatgcaat aaagaac
//