From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Tetrahymena pyriformis alpha-tubulin gene.
Date: 24 Jul 92 10:10:19 GMT
Distribution: bionet
Checksum: 04645     5

LOCUS       TETTUBA      1881 bp ds-DNA             INV       24-JUL-1992
DEFINITION  Tetrahymena pyriformis alpha-tubulin gene.
ACCESSION   X12767
KEYWORDS    alpha-tubulin; tubulin.
SOURCE      Tetrahymena pyriformis.
  ORGANISM  Tetrahymena pyriformis
            Eukaryota; Animalia; Protozoa; Ciliophora; Oligohymenophora;
            Hymenostomata; Hymenostomatida; Tetrahymenina; Tetrahymenidae.
REFERENCE   1  (bases 1 to 1881)
  AUTHORS   Barahona,I., Soares,H., Cyrne,L., Penque,D., Denoulet,P. and
            Rodrigues-Pousada,C.
  TITLE     Sequence of one alpha- and two beta-tubulin genes of Tetrahymena
            pyriformis
  JOURNAL   J. Mol. Biol. 202, 365-382 (1988)
  STANDARD  simple automatic
COMMENT     *source: strain=CGL; library=macronuclear DNA in pBR322; *source:
            clone=alpha-TT.
               EMBL features not translated to GenBank features:
               key        from     to       description
               PRM          91     98       pot. TATA-box
               PRM         168    173       put. TATA-box
               SITE        176    181       poly(AG) stretch
               TRANSCR     198      ?       pot. transcript
               TRANSCR     203      ?       pot. transcript
               SITE       1680   1686       pot. polyA signal
FEATURES             Location/Qualifiers
     CDS             301..1650
                     /note="alpha-tubulin (AA 1 - 449)"
                     /codon_start=1
                     /translation="MREVISIHVGQGGIQVGNACWELFCLEHGIQPDGQMPSDKTIGG
                     GDDAFNTFFSETGAGKHVPRAVFLDLEPTVIDEVRTGTYRQLFHPEQLISGKEDAANN
                     FARGHYTIGKEIVDLCLDRIRKLADNCTGLQGFLVFNSVGGGTGSGLGSLLLERLSVD
                     YGKKSKLGFTIYPSPQVSTAVVEPYNSILSTHSLLEHTDVAVMLDNEAIYDICRRNLD
                     IERPTYTNLNRLIAQVISSLTASLRFDGALNVDITEFQTNLVPYPRIHFMLSSYAPII
                     SAEKAYHEQLSVAEITNSAFEPANMMAKCDPRHGKYMACSMMYRGDVVPKDVNASIAT
                     IKTKRTIQFVDWCPTGFKVGINYQPSTVVPGGDLAKVMRAVCMISNSTAIAEVFSRLD
                     HKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKDYEEVGIETAEGEGEEEGY"
BASE COUNT      561 a    443 c    358 g    519 t
ORIGIN
        1 gatctcgttt gatatgaaac aagaagaaga aaaattaaaa aaagagtggg aagaaagaat
       61 tcaaattttt attttttgaa acgtatcaaa tattaataga tttgattaaa aggatgagca
      121 attatgaata ttgaaaataa tctgtgttgg gaagtgcaga aaaagtgatt taagaagaga
      181 gtgggttgag gcaagtcaat ataataatta ttaaatcaaa aaaaaaatcc taaaaagaaa
      241 cacaaactct taagcagtcc ctcaagtact cttaaagaat tatccttaga aaagttagaa
      301 atgagagaag ttatttcaat tcacgttggt caaggtggta tccaagtcgg taacgcctgt
      361 tgggaattgt tctgtcttga acacggtatt cagcctgatg gtcaaatgcc ctccgataag
      421 actatcggtg gtggtgatga tgctttcaac accttcttct ccgaaactgg tgccggtaag
      481 cacgtcccca gagctgtctt cttggatctc gaacctaccg ttatcgacga agtcagaacc
      541 ggtacttaca gacaattgtt ccaccccgaa caattgatct ccggtaagga agatgccgct
      601 aacaacttcg ccagaggtca ctacaccatt ggtaaggaaa tcgtcgatct ctgtttggac
      661 agaatcagaa agttggctga caactgtact ggtctccaag gtttcctcgt cttcaactcc
      721 gttggtggtg gtaccggttc cggtttgggt tctctcctct tggaaagact ctccgtcgat
      781 tacggtaaga agtccaagtt gggtttcacc atttaccctt ctccccaagt ttccaccgct
      841 gtcgtcgaac cttacaactc cattctttcc actcactccc tcttggaaca cactgatgtc
      901 gccgtcatgc tcgacaacga agctatctac gatatctgca gaagaaacct cgacatcgaa
      961 agacccacct acaccaactt gaacagactc atcgctcaag ttatctcttc cttgactgcc
     1021 tccctcagat tcgatggtgc ccttaacgtc gatatcactg aattccaaac caacttggtc
     1081 ccctatccca gaattcactt tatgctttca tcttacgctc ccatcatctc cgctgaaaag
     1141 gcctaccacg aacaactctc cgttgctgaa atcaccaact ccgccttcga acccgctaac
     1201 atgatggcca agtgtgaccc cagacacggt aagtacatgg cttgctctat gatgtacaga
     1261 ggtgacgtcg tccccaagga tgtcaacgct tccatcgcca ccatcaagac caagagaacc
     1321 atccaattcg tcgattggtg ccccactggt ttcaaggtcg gtattaacta tcaaccttcc
     1381 accgtcgttc ccggtggtga tctcgctaag gtcatgagag ctgtttgcat gatttccaac
     1441 tctactgcca tcgctgaagt cttctccaga ctcgatcaca agttcgatct tatgtacgcc
     1501 aagagagcct tcgtccattg gtacgtcggt gaaggtatgg aagaaggtga attctccgaa
     1561 gccagagaag atcttgctgc cctcgaaaag gattacgaag aggtcggtat tgaaaccgct
     1621 gaaggtgaag gtgaagaaga aggttactga tttgcttgat tcctttcatt aattctaaca
     1681 attaaatata actaatatat atcattaatt gcaatacata tccatcaaaa tgtcttcgca
     1741 tttaaaattt aggttcctgc taacacacaa aatatgcagg aaaccacaaa aaaaaattct
     1801 cattttcctc ttttcaaatt aaaaaggaca agttccaaac aaatactcaa tacactactg
     1861 tcttaatttt tcctttttct t
//