From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Pea gTUB1 gene for beta-tubulin 1
Date: 15 Sep 92 10:05:01 GMT
Distribution: bionet
Checksum: 62028     7

LOCUS       PEAGTUB01    3023 bp ds-DNA             PLN       15-SEP-1992
DEFINITION  Pea gTUB1 gene for beta-tubulin 1
ACCESSION   X54844
KEYWORDS    beta-tubulin-1; tubulin.
SEGMENT     1 of 3
SOURCE      Pisum sativum DNA.
  ORGANISM  Pisum sativum
            Eukaryota; Plantae; Embryobionta; Magnoliophyta; Magnoliopsida;
            Rosidae; Fabales; Fabaceae.
REFERENCE   1  (bases 1 to 3023)
  AUTHORS   Cerff,R.
  JOURNAL   Unpublished (1990)
  STANDARD  full staff_entry
REFERENCE   2  (sites)
  AUTHORS   Liaud,M.F., Brinkmann,H. and Cerff,R.
  TITLE     The beta-tubulin gene family of pea: primary structures, genomic
            organization and intron dependant evolution of genes
  JOURNAL   Plant Mol. Biol. 18, 639-651 (1992)
  STANDARD  full staff_review
COMMENT     *source: strain=Rosakrone; *source: stage=2 weeks old seedlings
            grown under; *source: tissue=green photosynthetic; *source:
            clone_library=MboI partials cloned in lambda EMBL4;
            Source: continuous white light.
            From EMBL    entry PSGTUB1;  dated 29-APR-1992.
FEATURES             Location/Qualifiers
     exon            <734..1127
                     /number=1
     CDS             join(734..1127,1748..2017,2125..2813)
                     /product="beta-tubulin 1"
                     /gene="gTUB1"
                     /codon_start=1
                     /translation="MRQILHIQGGQCGNQIGAKFWEVVCAEHGIDPTGRYTGDSDLQL
                     ERIDVYYNEASGGRFVPRAVLMDLEPGTMDSIRSGPYGQIFRPDNFVFGQSGAGNNWA
                     KGHYTEGAELIDSVLDVVRKEAENCDCLQGFQVCHSLGGGTGSGMGTLLISKIREEYP
                     DRMMLTFSVFPSPKVSDTVVEPYNATLSVHQLVENADEVMVLDNEALYDICFRILKLS
                     NPSFGDLNHLISATMSGVTCCLRFPGQLNSDLRKLAVNLIPFPRLHFFMVGFAPLTSR
                     GSQQYRTLSVPELTQQMWDAKNMMCAADPRHGRYLTASAMFRGKMSTKEVDEQMMNVQ
                     NKNSSYFVEWIPNNVKSTVCDIPPTGLKMASTFIGNSTSIQEMFRRVSEQFTAMFRRK
                     AFLHWYTGEGMDEMEFTEAESNMNDLVAEYQQYQDATADEDEYGEEEGDEEEYGQHDI
                     "
     intron          1128..1747
                     /number=1
     exon            1748..2017
                     /number=2
     intron          2018..2124
                     /number=2
     exon            2125..2813
                     /number=3
BASE COUNT      814 a    510 c    607 g   1092 t
ORIGIN
        1 cccttagacc aaaacccatg cacgctctca aaacctatta atatatctcc ttggaatata
       61 tcataaaata aaccttctaa atattcaaaa aaatttatct taaggtgttc agctaaccca
      121 tcatgagata cgtaaacact aataacatta aatatatccc gttcaactac aatttttttt
      181 taacaatatt tttcatttaa ttcggagtta gtcggcctaa ctgatttttc aacgagacga
      241 atgagacaat caaattattg aaattaagtt gaaaacttta ataacatcca cttaaaaatt
      301 attaaaataa atatttaata ttcataaaaa tttaacaatt atatttaagc aacaatgtaa
      361 aatatcctta cattaaatct gcatatcaat taaattaaaa atataattga tgtaaatatt
      421 atagaaaaat aaattatatt ttgaataagt atactaattt aaatcttatt ttggctctca
      481 aaatggtaag caatataaat ttcaaaattc aaaaatccat ttcaaaattc cagcgcttca
      541 tctaaataaa aaacagcaaa actaatccaa ccgttgaaac aaaaataaaa taatttccaa
      601 acgtttttta ctctctctct ataaaaccga atcattttcc ccaatctctc tctattcttc
      661 gcaccctagg aaccttttct ccccattttc gtttctaggt catttcttca atttcttctc
      721 tcaaaccttc aacatgcgtc aaatcctcca catccagggt ggtcaatgcg gcaaccaaat
      781 cggtgccaaa ttctgggaag tcgtttgcgc cgaacacgga atcgatccaa ccggacgcta
      841 caccggcgac tcagatcttc aactagagag gatcgatgtt tactacaacg aagcaagtgg
      901 tggccgcttc gttcccagag cggttctcat ggatcttgaa ccgggaacca tggacagtat
      961 ccggtctgga ccgtacggtc agatctttag accggataac tttgtgtttg gtcagagtgg
     1021 tgctgggaat aactgggcta aaggtcacta tactgaaggc gcggaactta ttgattctgt
     1081 tcttgacgtt gttcgtaagg aagctgaaaa ctgtgattgc ttgcagggta tggttttttt
     1141 aacttgttct gatatttagg gtttcgttga tttctttgat ttagggtttt gttgaactca
     1201 ttttgttgtt tcagatgcaa tgaagttgta gttgaagtgt tgatgcgttc ttgtagggtc
     1261 ttttgctgac tgtgaaatga gatctgtgtt tctctttttg aagttttgtc gctgattttg
     1321 agttggcgtg tttgtatatg tatagttgga atatttcatg tcttgatgct gcaatttggg
     1381 tatttgattt gtttactaac ttttttgagt ttaggttaag gttcttgatt tttgtgtttg
     1441 ttagggttaa cttttatagt ttttgtagct gataataaaa tagctgcagc gtgttttcct
     1501 gtgtgcttgt taactgtttt gttttaggtt ttagggttct tgatttttgt gtttgtatgt
     1561 gtttttcttt ttaagatttt gtggatgata atgaaatgac cgtggtgtgt ttctcctgtg
     1621 tgtttgttaa ctttttatga atttagtttt cagggttctt gagttttgtg tttgtcatag
     1681 tttacttttt ttttggcttt tgtggctgat tatgagggtg cggtgttttt tttcttcctg
     1741 tgtgcaggtt ttcaagtgtg tcattcactt ggtggaggaa ctggatctgg aatgggaacc
     1801 cttctgattt caaagattag ggaggaatac cctgacagga tgatgcttac tttctctgtt
     1861 ttcccttctc ctaaagtttc tgacactgtg gtggaaccct acaatgctac tctatctgtt
     1921 catcagcttg ttgaaaatgc agatgaggtt atggttcttg acaatgaagc cttgtatgac
     1981 atttgcttcc gtattctgaa gcttagtaac ccaagctgta agctagtttt ctttttcact
     2041 ctcctatgtt gacccttttc ttatgctagt catttcatgt gtttttttgg agtgtgtttg
     2101 atttgatttt ctgtgttgat gcagttggtg atctgaatca tttgatatca gcaactatgt
     2161 ctggtgtaac atgttgtttg aggttccctg gtcagttgaa ctctgatctc aggaagcttg
     2221 ctgtgaatct cattccattc cctcgtcttc actttttcat ggtggggttt gcacctctga
     2281 cctctcgcgg ctcccagcag tacaggactc tcagtgtccc tgagctcact caacaaatgt
     2341 gggatgccaa gaacatgatg tgtgctgctg atcctaggca tggacgttac ctcaccgcat
     2401 ctgctatgtt tagaggcaag atgagtacca aggaggttga tgaacaaatg atgaatgttc
     2461 agaacaagaa ctcgtcatac tttgtggaat ggattccaaa caatgtcaaa tccactgtgt
     2521 gtgatattcc cccaactggt ttgaagatgg cttcaacatt tattggaaac tcgacctcaa
     2581 ttcaagaaat gttccgcagg gtgagtgaac agtttactgc tatgttcagg aggaaggctt
     2641 tcttgcattg gtacacaggt gaaggaatgg atgagatgga attcactgag gctgagagca
     2701 acatgaatga tttggttgca gagtatcaac agtatcaaga tgcgactgct gatgaggatg
     2761 agtatggaga ggaggaaggc gatgaggaag agtatggtca acatgacatt tgattttgaa
     2821 tgaaagtttg aatattataa tctgctgcaa cttcatgttg ctttttcaaa ctaagactag
     2881 ttttcttatg tttcacttac tatggctgta aaattggttt tggtttattt gcttttatgt
     2941 gtgaccaatt ggtagttgag taagacagct taaacaattc cttcaagtat cactttgttg
     3001 ttagaattaa acatcaatat ttg
//