From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Plasmid pSa (from Escherichia coli) replicase (repA) and
Date: 16 Dec 92 23:25:26 GMT
Distribution: bionet
Checksum: 34805 5

LOCUS       PLPREPA      2491 bp ds-DNA             BCT       15-DEC-1992
DEFINITION  Plasmid pSa (from Escherichia coli) replicase (repA) and
            recombinase genes, complete cds.
ACCESSION   M95790
KEYWORDS    repA gene; replicase.
SOURCE      Plasmid pSa DNA.
  ORGANISM  Plasmid pSa
            Prokaryotae.
REFERENCE   1  (bases 1 to 2491)
  AUTHORS   Okumura,M. and Kado,C.I.
  TITLE     The region essential for efficient autonomous replication of pSa in
            E. coli
  JOURNAL   Mol. Gen. Genet. (1992) In press
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     RBS             683..687
                     /gene="repA"
     CDS             46..702
                     /note="ORF II"
                     /product="recombinase"
                     /codon_start=1
                     /translation="MPKYYAYLRVSRDGQDPENQKYGLLEYANAKGFAPLQIEEEIAS
                     RAKDWRKRKLGAIIEKAERGDVLLTPEITRIAGSALAALEILKAASERGLIVHVTKQK
                     IIMDGSLQSDIMATVLGLAAQIERHFIQARTTEALQVARERGKTLGRPKGSKSSALKL
                     DSRIDEVQAYVNLGLPQSRAAELLGVSPHTLRLFIKRRNIKPTNTRPTITMPGREQHA
                     "
     CDS             695..1666
                     /gene="repA"
                     /product="replicase"
                     /codon_start=1
                     /translation="MPKNNKAPGHRINEIIKTSLALEMEDAREAGLVGYMARCLVQAT
                     MPHTDPKTSYFERTNGIVTLSIMGKPSIGLPYGSMPRTLLAWICTEAVRTKDPVLNLG
                     RSQSEFLQRLGMHTDGRYTATLRNQAQRLFSSMISLAGEQGNDFGIENVVIAKRAFLF
                     WNPKRPEDRALWDSTLTLTGDFFEEVTRSPVPIRIDYLHALRQSPLAMDIYTWLTYRV
                     FLLRAKGRPFVQIPWVALQAQFGSSYGSRARNSPELDDKARERAERAALASFKYNFKK
                     RLREVLIVYPEASDCIEDDGECLRIKSTRLHVTRAPGKGARIGPPPT"
BASE COUNT      630 a    760 c    657 g    444 t
ORIGIN      
        1 gctagaatga ggcatgttta accgaatctg acgttttccc tgcaaatgcc aaaatactat
       61 gcctatctcc gggtttcgcg tgacggccaa gacccggaaa accaaaaata cggtttgctc
      121 gaatacgcga acgccaaagg cttcgcgccg ctacagatcg aggaagaaat tgccagcaga
      181 gcaaaggact ggcgcaagcg caagctcgga gcaatcatcg aaaaggccga gcgtggcgac
      241 gtgctactga cgccggagat tacgcgcatt gccggttccg ccctcgccgc cttggaaatt
      301 ctcaaagcgg cgagcgagcg cggcctaatc gtccatgtga ccaaacagaa gatcatcatg
      361 gacggcagcc tacaaagcga catcatggca accgtgcttg gcttggctgc acagatcgag
      421 cggcatttca ttcaggcacg taccaccgag gcgctacaag tcgccagaga gcgcggcaag
      481 acgctcgggc gacccaaggg cagcaaatcg agcgccttga agctggacag ccgtattgat
      541 gaagtacagg catacgtgaa ccttggcttg ccgcaaagtc gcgcagccga gttgttaggc
      601 gtcagccctc acaccttgcg cctgttcatc aaacgccgga acatcaaacc cacaaacact
      661 agaccaacca tcaccatgcc ggggagggaa caacatgcct aagaacaaca aagcccccgg
      721 ccatcgtatc aacgagatca tcaagacgag cctcgcgctc gaaatggagg atgcccgcga
      781 agctggctta gtcggctaca tggcccgttg ccttgtgcaa gcgaccatgc cccacaccga
      841 ccccaagacc agctactttg agcgcaccaa tggcatcgtc accttgtcga tcatgggcaa
      901 gccgagcatc ggcctgccct acggttctat gccgcgcacc ttgcttgctt ggatatgcac
      961 cgaggccgtg cgaacgaaag accccgtgtt gaaccttggc cggtcgcaat cggaatttct
     1021 acaaaggctc ggaatgcaca ccgatggccg ttacacggcc acccttcgca atcaggcgca
     1081 acgcctgttt tcatccatga tttcgcttgc cggcgagcaa ggcaatgact tcggcattga
     1141 gaacgtcgtc attgccaagc gcgcttttct attctggaat cccaagcggc cagaagatcg
     1201 ggcgctatgg gatagcaccc tcaccctcac aggcgatttc ttcgaggaag tcacccgctc
     1261 accggttcct atccgaatcg actacctgca tgccttgcgg cagtctccgc ttgcgatgga
     1321 catttacacg tggctgacct atcgcgtgtt cctgttgcgg gccaagggcc gccccttcgt
     1381 gcaaatccct tgggtcgccc tgcaagcgca attcggctca tcctatggca gccgcgcacg
     1441 caactcgccc gaactggacg ataaggcccg agagcgggca gagcgggcag cactcgccag
     1501 cttcaaatac aacttcaaaa agcgcctacg cgaagtgttg attgtctatc ccgaggcaag
     1561 cgactgcatc gaagatgacg gcgaatgcct gcgcatcaaa tccacacgcc tgcatgtcac
     1621 ccgcgcaccc ggcaagggcg ctcgcatcgg cccccctccg acttgaccag gccaacgcta
     1681 cgcttggctt ggtcaagcct tcccatccaa cagcccgccg tcgagcgggc ttttttatcc
     1741 ccggaagcct gtggatagag ggtagttatc cacgtgaaac cgctaatgcc ccgcaaagcc
     1801 ttgattcacg gggctttccg gcccgctcca aaaactatcc acgtgaaatc gctaatcagg
     1861 gtacgtgaaa tcgctaatcg gagtacgtga aatcgctaat aaggtcacgt gaaatcgcta
     1921 atcaaaaagg cacgtgagaa cgctaatagc cctttcagat caacagcttg caaacacccc
     1981 tcgctccggc aagtagttac agcaagtagt atgttcaatt agcttttcaa ttatgaatat
     2041 atatatcaat tattggtcgc ccttggcttg tggacaatgc gctacgcgca ccggctccgc
     2101 ccgtggacaa ccgcaagcgg ttgcccaccg tcgagcgccg agtcacccga agggccgcaa
     2161 cgtagtggag gacggcaacg ccgttgaacg gcgcgagcac tatggcacgc gaagcgagct
     2221 tgacacgatg gaggaaagac catgaccgac acgcgccgcg agcaggagaa agacgaacgc
     2281 cggaagctgc aagagcagtc gcgccagaat gaagcggaaa ccatgcgcct gctggctttc
     2341 gaggcaggcc gccaattggc cgaaattccg aaagaagcca aaggcaatga acccttgctt
     2401 gagaactaca agagcggact acaagagacc cgcaaagagc tagaaaccac gccagacgcc
     2461 actaagagca ccaacgccaa ccggcttgag c
//