From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: A.calcoaceticus AccI restriction-modification genes, complete cds.
Date: 5 Nov 92 23:05:04 GMT
Distribution: bionet
Checksum: 47594 8

LOCUS       ACCRMACCI    3353 bp ds-DNA             BCT       16-OCT-1992
DEFINITION  A.calcoaceticus AccI restriction-modification genes, complete cds.
ACCESSION   D10671
KEYWORDS    AccI methylase; AccI restriction endonuclease; deoxyribonuclease;
            methyltransferase; retrotransposon; reverse transcriptase;
            type II restriction-modification system.
SOURCE      Acinetobacter calcoaceticus (library: pUC18) DNA, clone pAccIRM8.
  ORGANISM  Acinetobacter calcoaceticus
            Prokaryotae; Gracilicutes; Scotobacteria; Neisseriaceae.
REFERENCE   1  (bases 1 to 3353)
  AUTHORS   Kawakami,B., Christophe,H., Nagatomo,M. and Oka,M.
  TITLE     Cloning and nucleotide sequences of the AccI
            restriction-modification genes in Acinetobacter calcoaceticus
  JOURNAL   Agric. Biol. Chem. 55, 1553-1559 (1991)
  STANDARD  full automatic
REFERENCE   2  (sites)
  AUTHORS   Hirochika,H. and Hirochika,R.
  TITLE     Retrotransposons are ubiquitous in plants
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
COMMENT     Submitted (08-JUL-1992) to DDBJ by: Keith Leppard N. Dept. of
            Biological Sciences University of Warwick Coventry CV4 7AL U.K.
            Phone:  203-523579 Fax:    203-523701 The complete sequence in
            reference [Virology 186, 280-285 (1992)] is available from
            Genbank/EMBL/DDBJ (accession number M73260).
FEATURES             Location/Qualifiers
     CDS             569..1669
                     /gene="R-AccI"
                     /product="AccI restriction endonuclease"
                     /codon_start=1
                     /translation="MDYYDRIRELTKNVPVELVDFEQPRDLARTPTQASSNFITNKEQ
                     GDWAEDLVTRAINENSKNFVAVKYGKSDNLVAGENGFDTFYQDFQTELDTIGKRPDLL
                     IFKKTDFDTTLGFDVSQIPHHQITDYVKKAIAGIEVRSSAFLIDKYEEAMQVRTQRFT
                     EIAFQTRDKILAEFLDVLDHPSRSKYITLLNTLTLETISIFDFKVPGWRSNERLIEVN
                     NLFKRLKVAIKEIQKRDYLSITPKVEDIKVVYKWIETFNVPHFYFQVFFDKVYGISFE
                     QILTIISNSDNDGVIFSVEKDVQNQNKTTIKINSKTGYPIASKVDEPTHESIRKEMDR
                     GRLLFYVTFKGGTAYLDLDNLRTILGIEEAEF"
     CDS             1669..3291
                     /note="consensus tetra-amino acids of N6-adenine
                     methylases, NPPY, at nt 2035-2046"
                     /gene="M-AccI"
                     /product="AccI methylase"
                     /codon_start=1
                     /translation="MIKTTEHIVNNSIERDYSKSISLEHRKKFAQFFTPFPIAYAMAK
                     WILGNKQLKTVLEPAFGLGVFSRAILSQQKEINIKAFEVDETIFENAKEYFDDFENVN
                     ILLQDYMYNDWKNKYDGIICNPPYFKFHDYDNKNILKEIETNLKCKLNGFTNLYTLFL
                     LKSIHQLSQNGRCAYIIPSEFLNSDYGKLVKTYLIKSKTLRHIIVIDFEENVFDDALT
                     TASIILCANDNITDKVQFNNIQSLQDLSKIDEIINKYPNFLETEQTYNFSDLNPEIKW
                     KAYYQKQNSIKFKNLVPFSTYAKVVRGIATGSNEYFTFNLSKAKEFNIDEQYLLPCIC
                     SAKDAKTSFFTKQDFEELKKSDKSVFLFNAQNSTDKNISSYIQKGESEEINKRFLTAS
                     RTPWYSLENRKPAPIWVSVFNRSGLRFIRNEANISNLTSYHCIIQNKQVVSEIDIDLL
                     FAYLLTDTAKQIFEDNSRQYGNGLQKFEPNDLNKGMMLDLGLLDKQTSDEILNLYKEY
                     KYLILDNKNGDEIINKIDKILTDKYSEKKHWA"
BASE COUNT     1262 a    507 c    516 g   1068 t
ORIGIN      
        1 ggatccaatt ttgtaggaat tttcccgaaa tgtagcgaca ccgcagcgac gtgtctggag
       61 taattttgaa aggtactttg gcttcttccc aacaccgaaa ccgtaccttc aaacctttgc
      121 aaaagctccg caaatcccgg aacttcgcgc tttgcctgat tgatgatttt gttttctcta
      181 agctcttcta ccctcttttt tttgttgcca ttttactgat ttttaattag ttttacaaag
      241 taactaaata tggcgctgag aaagctaccg aagttttagt tcaacatagg ttttgcaaaa
      301 tgcggggata agtctaagtt gaactatttt agatatttat ccgcaaattg gcttttcatc
      361 ttgatttttt ttattaattt aacaatctga aaaagccatt tgctacgttt ctgaattctt
      421 gaacttttgg ttcttcgaat tcccgcactt cgcaaagcct ttttccgttt atgcccaatg
      481 ctatgagcaa tcgcacaaat actaacccac aataaaaacc gacaaaaaaa gtttaacttt
      541 gtagaataaa gtttcataat aaaagaagat ggattattac gacagaatta gagaattaac
      601 aaaaaatgtt cctgtagaat tggttgattt tgaacaacca agagacttgg caagaacacc
      661 aacacaagca tcttcaaatt ttattacaaa taaagagcaa ggtgattggg ctgaagacct
      721 tgttacaagg gcaattaatg aaaattctaa aaattttgta gctgtaaaat atgggaaatc
      781 tgacaattta gttgcaggag aaaatggctt tgatactttt tatcaagatt ttcaaactga
      841 acttgacacc ataggaaaac gccccgactt gttaatcttt aaaaagacag attttgatac
      901 tactttaggt tttgatgtta gtcaaattcc acatcatcaa atcactgatt atgtaaaaaa
      961 agcaattgca ggaattgaag ttcgctcaag tgcttttttg attgacaagt acgaagaagc
     1021 aatgcaagta agaactcaaa ggtttactga aattgcattt caaacaagag ataaaatatt
     1081 agccgaattt ttagatgttt tagaccatcc aagtagaagt aagtacatca ctttattaaa
     1141 tactttaacc cttgaaacta tttcaatttt tgactttaaa gttccaggtt ggaggtctaa
     1201 tgaaagactt atagaagtaa ataatttatt caaaagacta aaggttgcaa taaaagaaat
     1261 tcaaaagaga gattatcttt caataacacc aaaagttgaa gacataaaag tggtttacaa
     1321 atggattgaa acattcaatg ttccacattt ttactttcaa gtgtttttcg acaaagtgta
     1381 tggaatttca tttgaacaaa ttctaactat aatttcaaat tctgataatg atggtgtaat
     1441 tttttccgtt gaaaaagatg tacaaaatca aaataaaaca acgataaaaa tcaactcaaa
     1501 aactggttat ccaattgctt caaaggttga tgaaccaact cacgaaagca tcaggaaaga
     1561 aatggacaga ggtcgattat tattttatgt aactttcaaa ggtggaactg catatcttga
     1621 tttagataat ttacgaacta ttttaggaat agaagaagct gaattttaat gataaaaaca
     1681 acggaacata ttgtcaataa ttccattgaa agagattact caaaatcaat ttcattagaa
     1741 catcgtaaaa aattcgcaca attttttacg cctttcccca ttgcttatgc aatggctaaa
     1801 tggattttag gcaacaaaca attaaaaacc gttcttgaac ctgctttcgg tcttggcgtt
     1861 ttttcaagag cgattttaag ccaacagaaa gaaatcaata taaaagcttt tgaagtagat
     1921 gaaacgatat ttgaaaacgc aaaagaatat tttgatgatt ttgaaaatgt aaatatactt
     1981 ttgcaagatt atatgtacaa tgattggaaa aacaaatatg atggaattat ttgcaatccg
     2041 ccctacttta agtttcacga ttatgacaat aaaaatattt taaaagaaat tgaaactaat
     2101 cttaaatgca agctgaatgg ctttacaaat ctttatactt tatttttact aaaatcaatt
     2161 catcagttaa gtcaaaatgg acgttgtgca tatataattc cttccgaatt tttgaattct
     2221 gattacggaa aattagtgaa aacttattta ataaaaagta aaacattacg gcacattatt
     2281 gtaattgact ttgaagaaaa tgtatttgat gatgctttaa caaccgcatc aattattctt
     2341 tgtgcaaatg ataatataac agataaagtt cagtttaaca atatccaatc attgcaagat
     2401 ttaagcaaaa tagatgaaat aataaataaa tatcctaact ttttagaaac cgaacagacc
     2461 tataattttt ctgatttaaa tccagaaatt aaatggaagg cttactatca aaaacaaaat
     2521 agcattaagt ttaaaaatct tgtgccattt tcgacttacg caaaagtagt tcgtggtata
     2581 gcaacaggtt caaatgaata ttttactttt aatctatcaa aagccaaaga atttaatatt
     2641 gacgaacaat atttattgcc ttgtatttgt agtgcaaaag atgctaaaac atcatttttc
     2701 actaaacaag attttgaaga attaaagaaa agtgataaat ctgtttttct tttcaatgct
     2761 caaaattcaa cggataaaaa tataagttca tatatccaaa aaggtgaaag cgaagagata
     2821 aataaacggt ttcttactgc aagtagaaca ccttggtatt cattagaaaa tagaaaacct
     2881 gcacccattt gggtttctgt ttttaatagg tcaggtttgc ggtttattag aaatgaagca
     2941 aatatttcta acctaacttc ttatcattgc attatccaaa acaaacaagt tgtttcagaa
     3001 attgacattg atttactttt tgcttatcta ttaacagaca cagctaaaca aatttttgaa
     3061 gacaacagtc gtcaatacgg taatggactt caaaaatttg aaccaaatga ccttaataaa
     3121 ggaatgatgc ttgatttagg attacttgac aagcagacat cagatgaaat tttaaatctt
     3181 tacaaggaat acaaatactt aatccttgac aataaaaacg gagacgaaat aattaataaa
     3241 atagataaaa tattaaccga caaatacagc gaaaaaaagc actgggcata ataactaagt
     3301 tttcgtcttg tccgaaggac agcgagatga aaaaccctgt tgaacggaag ctt
//