From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: S.gairdneri CYP17 mRNA for steroid 17-alpha-monooxygenase
Date: 10 Sep 92 10:36:05 GMT
Distribution: bionet
Checksum: 53748     6

LOCUS       SMOCYP17R    2287 bp ss-RNA             VRT       10-SEP-1992
DEFINITION  S.gairdneri CYP17 mRNA for steroid 17-alpha-monooxygenase
ACCESSION   X65800
KEYWORDS    CYP17 gene; steroid 17-alpha-monooxygenase.
SOURCE      Salmo gairdneri RNA.
  ORGANISM  Salmo gairdneri
            Eukaryota; Animalia; Chordata; Vertebrata; Osteichthyes;
            Actinopterygii; Salmoniformes; Salmonoidei; Salmonidae.
REFERENCE   1  (bases 1 to 2287)
  AUTHORS   Sakai,N.
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
REFERENCE   2  (bases 1 to 2287)
  AUTHORS   Sakai,N., Tanaka,M., Adachi,S., Miller,W.L. and Nagahama,Y.
  TITLE     Rainbow trout cytochrome P-450c17 (17alpha-hydroxylase/17,
            20-lyase): cDNA cloning, enzymatic properties and temporal pattern
            of ovarian P-450c17 mRNA expression during oogenesis
  JOURNAL   FEBS Lett. 301, 60-64 (1992)
  STANDARD  full automatic
COMMENT     *source: tissue=ovarian thecal cell layer; *source: sex=Female;
            *source: is_macronuclear=N; *source: is_proviral=N; *source:
            is_germline=N;
            From EMBL    entry SGCYP17R;  dated 18-AUG-1992.
FEATURES             Location/Qualifiers
     CDS             48..1592
                     /EC_number="1.14.99.9"
                     /note="cytochrome P450XVIIA1"
                     /product="steroid17-alpha-monooxygenase"
                     /gene="CYP17"
                     /codon_start=1
                     /translation="MAWFLCMCVFSVVGLGLLLLQVKLRRSLETRGGPPSLPVFPLIG
                     SLLSLRSNQAPHVLFQKLQQKYGHTYSLMMGPHTVILVNHHQHAKEVLLKKGKIFAGR
                     PRTVTTDLLTRDGKDIAFADYGATWRFHRKTVHGALCMFGEGSASIEKIICREALSLC
                     DTLRESGSASLDLSPELTRAVTNVVCSLCFSSSYCRGDPEFEAMLQFSQGIVDTVAKD
                     SLVDIFPWLQVFPNADLRLLKQCVSIRDKLLQKKYEEHKSDYSDHEQRDLLDALLRAK
                     RSAENNNTAEITMETVGLSEDHLLMTVGDIFGAGVETTSTVLKWAIAYLIHHPQVQQR
                     IQEELDSVVGGDRTPQLSDRGSLPYLEATIREVLRIRPVAPLLIPHVAQTDTSIGKFT
                     VRKGARIIINLWSLHHDEKEWKNPEMFDPGRFLNEEGTGLCIPSPSYLPFGAGVRVCL
                     GEALAKMEIFLFLSWILQRLTMTVSPGQPLPSLEGKFGVVLQPVKYKVNATPRAGWEK
                     SHLQTS"
BASE COUNT      577 a    570 c    580 g    560 t
ORIGIN
        1 gtgacactct gataacgacg ccagcccctc tcggcccaac atacagcatg gcgtggtttt
       61 tgtgcatgtg tgtattctct gtggtcgggc tgggtctact gctcttgcag gtgaaacttc
      121 gaaggtccct ggagaccaga gggggccctc cgagcctgcc cgtgttccca ctcatcggga
      181 gcctgttgag cctgcgaagc aatcaggcac cgcatgtcct cttccagaag ctgcagcaga
      241 aatatggaca tacctattct ctaatgatgg gcccacacac agtcatcctt gtcaaccacc
      301 accagcacgc caaagaggtc ctccttaaaa aggggaaaat ctttgcagga agacccagaa
      361 cagtaaccac agacctgttg acgagggacg gtaaagatat tgcctttgca gactacggtg
      421 ccacttggag atttcatcgt aaaacagtgc atggagcctt gtgtatgttt ggtgagggct
      481 ctgcctccat cgagaagatt atctgtaggg aagctctctc cctgtgtgac actctgcgag
      541 agtcaggaag tgcatcgttg gacttgtccc cagagctgac aagagctgtc accaacgtgg
      601 tctgctctct ctgcttcagc tcctcctatt gccgcggcga ccccgagttt gaggccatgc
      661 tgcagttcag ccagggcatc gtggatacgg tcgctaagga cagtctggtg gacatcttcc
      721 catggctaca ggtcttcccc aacgcagacc tgcgtctcct aaagcagtgt gtgtcaatca
      781 gagacaagct gcttcagaaa aaatacgagg aacacaagtc ggactacagc gatcatgaac
      841 agagagacct gctggacgcc ctgctaaggg ccaaacgcag tgctgagaac aacaacactg
      901 ccgagatcac catggagacc gtgggcctta gtgaagacca cctgctcatg accgtgggtg
      961 acatatttgg agccggagtg gaaaccacgt caacagtcct aaaatgggca attgcctacc
     1021 tcatccacca tccacaggta caacagcgaa ttcaggagga actggacagt gtggtggggg
     1081 gagacagaac cccccagctc agtgacaggg ggagcctgcc ctacctggag gccaccatta
     1141 gagaggtgct acgcatccgg cctgttgccc ctctactcat cccccatgta gcccaaacag
     1201 acaccagtat tggcaagttc actgttagga aaggtgctcg catcatcatc aacttgtggt
     1261 ccctgcatca cgatgagaag gagtggaaga acccagagat gtttgaccct ggtcgcttcc
     1321 tgaacgagga ggggacaggt ctgtgcatcc cctcgcccag ctacttgccg tttggggcag
     1381 gggtgagagt gtgtctgggg gaggcgctgg ctaagatgga gatcttcctc ttcctctcct
     1441 ggatccttca gcgtttaact atgaccgtgt ccccgggcca gccactgccc agcctggaag
     1501 gaaagtttgg ggtagtcctc cagccggtca aatacaaggt caatgccaca ccaagagcag
     1561 gctgggagaa gagccatctc cagacctctt agaccccccc ccccctccac ccttaacgaa
     1621 tgacatgcat gggtgttaat gtttaaacac taatagtggt gcatttgaat tgaccttgga
     1681 atctcatagc tccagttggt caagttgtga agatgatata aatgcgttct agtgcttttg
     1741 aggttggtac aactctacaa tgaattagat ttttagctag agcgctaagc aagatagtgt
     1801 tgctaataat aagttggtta actagctgac aatgtgtcaa accatagact gtattggtca
     1861 aagtagcttc tttatccaca tagctaaagg cattgtttta ccatcttttg gtcgcaaagg
     1921 tgacaggtca ttgatgaaac gtcagaactc gatgatactc aggtatcatc attctggcct
     1981 gagtttttca cttggaactt ggagggcatt gacttggagg gcattgtact ggctattttg
     2041 gagtcgggaa ctcgtatttc cacatttcag agagctctcg aatgcaccat agaacatgga
     2101 aacaggcgtg aactaaggtt gtgatacatt acgcagagac agcccttgta atgactgcta
     2161 tttttcactg tattgatgtt gaacaatgct gtatattttt ttgccttaat gagctgtact
     2221 aatgtattcc gttactacag ttttacataa tactgtcttt cagtgatgaa taaaaaatca
     2281 tatggca
//