From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: S.mutans GS-5 scrB gene for sucrose-6-phosphate hydrolase activity
Date: 5 Jan 93 06:49:00 GMT
Distribution: bionet
Checksum: 57882 5

LOCUS       STRSCRBA     2025 bp ds-DNA             BCT       04-JAN-1993
DEFINITION  S.mutans GS-5 scrB gene for sucrose-6-phosphate hydrolase activity
ACCESSION   X51507
KEYWORDS    sucrose-6-phosphate hydrolase.
SOURCE      Streptococcus mutans DNA.
  ORGANISM  Streptococcus mutans
            Prokaryotae; Firmicutes; Firmibacteria; Gram-positive cocci;
            Streptococcaceae.
REFERENCE   1  (bases 1 to 2025)
  AUTHORS   Sato,Y. and Kuramitsu,H.K.
  TITLE     Sequence analysis of the Streptococcus mutans scrB gene
  JOURNAL   Infect. Immun. 56, 1956-1960 (1988)
  STANDARD  full automatic
COMMENT     *source: strain=GS-5;
               From EMBL    entry SMSCRBA;  dated 18-DEC-1992.
FEATURES             Location/Qualifiers
     -35_signal      171..176
                     /gene="scrB"
     -10_signal      194..199
                     /gene="scrB"
     RBS             217..222
                     /gene="scrB"
     CDS             232..1596
                     /gene="scrB"
                     /product="sucrose-6-phosphate hydrolase"
                     /codon_start=1
                     /translation="MNLPQNIRYRRYQDWTEEEIKSIKTNVALSPWHTTYHIEPKTGL
                     LNDPNGFSYFNGKFNLFYQNWPFGAAHGLKSWIHTESEDLVHFKETGTVLYPDTSHDS
                     HGAYSGSAYEIGDQLFLFYTGNVRDENWVRHPLQIGAFMDKKGNIQKFTDVLIKQPND
                     VTEHFRDPQIFNYKGQFYAIVGAQSLDFGGSKSEYMIECPNLVFINEQPVLIYSPQGL
                     SKSELDYHNIYPNTYKVCQSFDTEKPALVDASEIQNLDFGFECYATQAFNAPDGRVYA
                     VSWIGLPDIDYPSDSYDYQGALSLVKELSLKHGKLYQYPVEAVRSLRSEKEAVTYKPE
                     TNNTYELELTFDSSSVNELLLFADNKGNGLAITVDTKMGTILIDRSKAGEQYALEFGS
                     QRSCSIQAKETVVNIFVDKSIFEIFINKGEKVFTGRVFPNDKQTGIVIKSGKPSGNYY
                     ELKY"
BASE COUNT      649 a    366 c    355 g    655 t
ORIGIN      
        1 ctgtgatgac ttcactagct actttgctat aatccatttg caaaaaactc cttaataatt
       61 attttgtttt gaaagcgttt ttttactttc cacgttattc attttatcat tttttccaat
      121 atgtcaatcg ttttacataa aattcttttt tgcaacaaaa gattgatttt tagaaagaaa
      181 acgtttacta tattagtatg tagataaact attattagga gtagataaac gatgaatttg
      241 cctcaaaata tcagatatcg ccgctatcaa gattggactg aagaagaaat aaaaagtatt
      301 aaaaccaatg tagctttgtc tccttggcat acaacgtatc atatagaacc taaaacagga
      361 ctccttaatg atccaaacgg tttttcctat tttaatggaa aatttaacct tttttatcaa
      421 aattggccat ttggagcagc tcacggctta aaatcttgga tccatactga aagtgaagac
      481 ttagtccatt tcaaagaaac aggtacagtc ctttatcccg atacttccca tgacagccat
      541 ggtgcatact cgggcagtgc ctatgaaatc ggtgatcagc tttttctctt ttatacagga
      601 aatgtccgag atgaaaattg ggttcgtcat ccacttcaaa tcggcgcttt tatggataaa
      661 aaaggtaata tccaaaaatt tactgatgtc cttattaaac agccaaatga tgttactgaa
      721 cactttcgcg atccccaaat ttttaattat aaaggacaat tttatgctat tgttggagca
      781 caaagtctag actttggcgg aagtaaatct gagtatatga ttgagtgccc aaatcttgtt
      841 tttataaacg aacagcctgt cctgatttat agtcctcagg gactcagtaa atctgaatta
      901 gattatcata atatttatcc taatacttac aaagtatgtc aatcgtttga cacagaaaag
      961 cctgccctag ttgatgcatc ggaaattcaa aatcttgact tcggatttga atgttatgct
     1021 acccaagctt tcaatgctcc tgatggccgt gtttatgctg tctcatggat tgggttacca
     1081 gatattgatt atccaagtga ttcatacgac tatcaaggag ctttgagcct cgtcaaagag
     1141 ctaagcctta aacacggtaa actctatcaa tatcccgttg aagctgttcg ttcattacgt
     1201 tctgaaaaag aagcagtcac ttacaagcca gaaaccaata atacttatga attagagtta
     1261 acttttgact cttcatcagt taatgaattg ctcctttttg ctgataataa aggcaatggt
     1321 ctagctatta cagttgatac taagatggga accattctga tcgatcgctc taaagcaggg
     1381 gagcaatatg ccttagaatt tggaagccaa cgttcttgct ctatccaagc aaaagagact
     1441 gttgtcaata tttttgttga caaatctatt tttgaaattt ttattaataa gggagaaaaa
     1501 gtttttactg gacgtgtttt tccaaatgac aaacaaactg gtattgtgat taaatctgga
     1561 aagccaagcg gtaattacta cgaattgaaa tattaactat ggttgcaaaa ttaacagatg
     1621 tcgcaaaact cgctggcgta agtccaacaa ccgtttcacg cgtgattaat cgaaaaggct
     1681 atctctctga aaaaacaatt actaaagtac aggctgccat gaaaactcta ggatacaagc
     1741 ccaataatct cgctcgcagc cttcagggga aatctgccaa gctaattgga cttattttcc
     1801 ctaatatcag tcacatcttc tattctgaac ttattgaata tttagaaata gagttgttta
     1861 aacatggcta caaagccatt atttgtaaca gtcagaataa tcccgataaa gaacgggatt
     1921 atctcgaaat gttagaagct aatcaggttg ggtgatgctg ccaacttact gatttagtgt
     1981 atgatggtgt ttttgaggtg ctccagtggc ttctgtttct atcag
//