From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Bacteriophage P1 replication region including repA, parA, and parB
Date: 27 Jan 93 05:51:03 GMT
Distribution: bionet
Checksum: 17662 14

LOCUS       PP1REP       6023 bp ds-DNA             PHG       26-JAN-1993
DEFINITION  Bacteriophage P1 replication region including repA, parA, and parB
            genes and incA, incB, and incC incompatibility determinants.
ACCESSION   K02380 X02954
KEYWORDS    incompatibility determinant; incompatibility determinant incA;
            incompatibility determinant incC; parA gene; parB gene; repA gene.
SOURCE      Bacteriophage P1 DNA from Escherichia coli K12, replicon
            lambda-P1:5R, clone pALA136.
  ORGANISM  Bacteriophage P1
            Viridae; ds-DNA nonenveloped viruses; Myoviridae.
REFERENCE   1  (bases 1 to 6023)
  AUTHORS   Abeles,A.L., Snyder,K.M. and Chattoraj,D.K.
  TITLE     P1 plasmid replication: Replicon structure
  JOURNAL   J. Mol. Biol. 173, 307-324 (1984)
  STANDARD  full automatic
REFERENCE   2  (bases 1 to 6023)
  AUTHORS   Abeles,A.L., Friedman,S.A. and Austin,S.J.
  TITLE     Partition of unit-copy miniplasmids to daughter cells
  JOURNAL   J. Mol. Biol. 185, 261-272 (1985)
  STANDARD  full automatic
COMMENT     The lambda-P1:5R replicon contains a 2081 bp fragment essential for
            plasmid replication. Within this fragment are several ORFs, the
            largest being the repA gene.  No product has been observed from the
            ORF located between bp 252-687 when mini-plasmids are present in
            maxi-cells. Another ORF between 2351-1890 does not code for a
            protein needed for replication or equipartition.
               The repA gene is flanked on either side by a set of 19 bp
            repeats that exert P1 incompatibility. The upstream set, incC,
            contains five sets of direct repeats, all oriented in the same
            direction, with spacers of 2-3 bp so as to be two turns of the
            helix apart (within bases 846-949). The downstream set, incA,
            contains nine sets of repeats, three in one orientation and six in
            the other. They are separated by 11-12 or 21-22 bp so that they are
            separated by three or four turns of the helix (within bases
            1866-2150).
               The incA determinant resides in or near the region essential for
            plasmid replication. It is dispensible for partition. incC
            resembles incA in structure and function.
FEATURES             Location/Qualifiers
     misc_feature    846..949
                     /note="incC incompatibility determinant region"
     misc_feature    1866..2150
                     /note="incA incompatibility determinant region"
     -35_signal      2341..2347
                     /gene="parA"
                     /note="putative"
     -10_signal      2364..2369
                     /gene="parA"
                     /note="putative"
     repeat_region   2370..2411
                     /rpt_type=inverted
                     /rpt_type=tandem
                     /rpt_unit=2370..2390
                     /rpt_unit=2391..2411
                     /gene="parA"
     RBS             2411..2414
                     /gene="parA"
     -35_signal      3589..3594
                     /gene="parB"
                     /note="putative"
     -10_signal      3612..3617
                     /gene="parB"
                     /note="putative"
     RBS             3626..3630
                     /gene="parB"
     misc_feature    4679..4790
                     /note="incB incompatibility determinant region;
                     centromere-like"
     repeat_region   4693..4736
                     /rpt_type=inverted
                     /rpt_type=tandem
                     /rpt_unit=4693..4714
                     /rpt_unit=4715..4736
     misc_feature    4737..4771
                     /note="CT/AT-rich region"
     CDS             1006..1866
                     /gene="repA"
                     /codon_start=1
                     /translation="MNQSFISDILYADIESKAKELTVNSNNTVQPVALMRLGVFVPKP
                     SKSKGESKEIDATKAFSQLEIAKAEGYDDIKITGPRLDMDTDFKTWIGVIYAFSKYGL
                     SSNTIQLSFQEFAKACGFPSKRLDAKLRLTIHESLGRLRNKGIAFKRGKDAKGGYQTG
                     LLKVGRFDADLDLIELEADSKLWELFQLDYRVLLQHHALRALPKKEAAQAIYTFIESL
                     PQNPLPLSFARIRERLALQSAVGEQNRIIKKAIEQLKTIGYLDCSIEKKGRESFVIVH
                     SRNPKLKLPE"
     CDS             2424..3620
                     /gene="parA"
                     /note="possible alternate translation start at bp 2492"
                     /codon_start=1
                     /translation="MSDSSQLHKVAQRANRMLNVLTEQVQLQKDELHANEFYQVYAKA
                     ALAKLPLLTRANVDYAVSEMEEKGYVFDKRPAGSSMKYAMSIQNIIDIYEHRGVPKYR
                     DRYSEAYVIFISNLKGGVSKTVSTVSLAHAMRAHPHLLMEDLRILVIDLDPQSSATMF
                     LSHKHSIGIVNATSAQAMLQNVSREELLEEFIVPSVVPGVDVMPASIDDAFIASDWRE
                     LCNEHLPGQNIHAVLKENVIDKLKSDYDFILVDSGPHLDAFLKNALASANILFTPLPP
                     ATVDFHSSLKYVARLPELVKLISDEGCECQLATNIGFMSKLSNKADHKYCHSLAKEVF
                     GGDMLDVFLPRLDGFERCGESFDTVISANPATYVGSADALKNARIAAEDFAKAVFDRI
                     EFIRSN"
     CDS             3637..4638
                     /gene="parB"
                     /codon_start=1
                     /translation="MSKKNRPTIGRTLNPSILSGFDSSSASGDRVEQVFKLSTGRQAT
                     FIEEVIPPNQVESDTFVDQHNNGRDQASLTPKSLKSIRSTIKHQQFYPAIGVRRATGK
                     IEILDGSRRRASAILENVGLRVLVTDQEISVQEAQNLAKDVQTALQHSIREIGLRLMR
                     MKNDGMSQKDIAAKEGLSQAKVTRALQAASAPEELVALFPVQSELTFSDYKTLCAVGD
                     EMGNKNLEFDQLIQNISPEINDILSIEEMAEDEVKNKILRLITKEASLLTDKGSKDKS
                     VVTELWKFEDKDRFARKRVKGRAFSYEFNRLSKELQEELDRMIGHILRKSLDKKPKP"
     CDS             4796..5422
                     /note="unidentified reading frame"
                     /codon_start=1
                     /translation="MTFNKISSEKGFTQVPLCLRKSKLSNLREYQNRIADIAKRSKAV
                     LGWASTAQFGTDNQFIKDDAARAASILEAARKDPVFAGISDNATAQIATAWASALADY
                     AAAHKSMPRPEILASCHQTLENCLIESTRNSMDATNKAMLESVAAEMMSVSDGVMRLP
                     LFLAMILPVQLGAATADACTFIPVTRDQSDIYEVFNVAGSSFWFLCCW"
BASE COUNT     1664 a   1374 c   1411 g   1574 t
ORIGIN      1 bp upstream of HincII site
        1 attcgaaaac aaactggttg acggacgggt aatgtcgcca gcgtattcac tgctgacact
       61 cagcggttgc cccggtcgat cagcgaattt aacgccgacg cccgacaaag gttaaccacc
      121 ctgcccccaa aagtgcattt atgtgtagga ctgccagagt ttagcagtga atattttgac
      181 tgatctgcca gcgtatatcc tcgatacaga aacgatgctg atggggatta acaggaagaa
      241 tcgcaacgtt aatgattaca accgagctat tagcggtaac taaaagggat ttttatgtct
      301 gataaagtaa cagtaaagca aactatcaac aaagcgactt caatctacaa aattgagcaa
      361 atcactgttg gcaggccagg atctgaacaa taccgtcgtg ctttcgagct tgccgatcag
      421 cttggtttaa aacacccgga ttgcattgag catgtatttc cgacctatgc tgatgagcaa
      481 tgtactcatg ttcttaccga agaggatttt ttcagcactg aagaacgaga aggcgttgat
      541 cgctgcattg gtgtgatttg ttcttcggta agtgatgagt tattccctaa tgtgcctgaa
      601 tatggtggta ttggatacca attcctgtac gagggcgatg agcttaaatg ctatgaacat
      661 ggtcttctca tcgaaagcgt agaataatac gactcccttc caaccggcta cgttggccgg
      721 tttttcactt atccacatta tccactggat agatccaata atcaggtcca tacagatccc
      781 aattagatcc atatagatcc ctgatcgttg caggccgcgc cacgtctggc ttagaagtgt
      841 atcgcgatgt gtgctggagg gaaaacgatg tgtgctggag ggataaaaat gtgtgctgac
      901 gggttgctaa tgtgtgctgg cgggatatag gatgtgtgtt gacgggaaag cttgggtagt
      961 tatcaccact tataaaaact atccacacaa ttcggaaaaa gtaatatgaa tcaatcattt
     1021 atctccgata ttctttacgc agacattgaa agtaaggcaa aagaactaac agttaattca
     1081 aacaacactg tgcagcctgt agcgttgatg cgcttggggg tattcgtgcc gaagccatca
     1141 aagagcaaag gagaaagtaa agagattgat gccaccaaag cgttttccca gctggagata
     1201 gctaaagccg agggttacga tgatattaaa atcaccggtc ctcgactcga tatggatact
     1261 gatttcaaaa cgtggatcgg tgtcatctac gcgttcagca aatacggctt gtcctcaaac
     1321 accatccagt tatcgtttca ggaattcgct aaagcctgtg gtttcccctc aaaacgtctg
     1381 gatgcgaaac tgcgtttaac cattcatgaa tcacttggac gcttgcgtaa caagggtatc
     1441 gcttttaagc gcggaaaaga tgctaaaggc ggctatcaga ctggtctgct gaaggtcggg
     1501 cgttttgatg ctgaccttga tctgatagag ctggaggctg attcgaagtt gtgggagctg
     1561 ttccagcttg attatcgcgt tctgttgcaa caccacgcct tgcgtgccct tccgaagaaa
     1621 gaagctgcac aagccattta cactttcatc gaaagccttc cgcagaaccc gttgccgcta
     1681 tcgttcgcgc gaatccgtga gcgcctggct ttgcagtcag ctgttggcga gcaaaaccgt
     1741 atcattaaga aagcgataga acagcttaaa acaatcggct atctcgactg ttctattgag
     1801 aagaaaggcc gggaaagttt tgtaatcgtc cattctcgca atccaaagct gaaactcccc
     1861 gaataagtgt gtgctggagg gaaaccgcat taaaaagatg tgtgctgccg ggaaggcttg
     1921 tccaatttcc tgtttttgat gtgcgctgga gggggacgcc cctcagtttg cccagacttt
     1981 ccctccagca cacatctgtc catccgcttt tccctccagt gcacatgtaa ttctctgcct
     2041 ttccctccag cacacatatt tgataccagc gatccctcca cagcacataa ttcaatgcga
     2101 cttccctcta tcgcacatct tagactttta ttctccctcc agcacacatc gaagctgccg
     2161 ggcaagccgt tctcaccagt tgatagagag tgaagcttgg ctgcccattg aagcaggaaa
     2221 tcaccaaaat gattcaggct acaacctgaa cgtagaagaa atccgcgtcc tttatgcgtg
     2281 gaggatgcca aagcatgttg tgacacactt ggcaaaggag taagcatgca gagaatgcta
     2341 tgtacaagca tctacgcata cattattatt ttatgcagca tttttaatta aattcaaaaa
     2401 tacagcataa aggatgactt tcgatgagtg attccagcca gcttcacaag gttgctcaaa
     2461 gagcaaacag aatgctcaat gttctgactg aacaagtaca gttgcaaaag gatgagctac
     2521 acgcgaacga gttttaccag gtctatgcga aagcggcact ggcaaaattg cctctactga
     2581 ctcgagcgaa cgttgactat gccgtaagtg aaatggaaga aaagggttat gttttcgata
     2641 aacgccctgc tggctcttca atgaaatatg cgatgtcaat tcagaacatc attgacatat
     2701 atgaacatcg cggagtgcca aaataccggg atcgctacag cgaagcgtat gtgattttca
     2761 tctccaatct taaaggcggt gtgtcaaaaa ctgtatcgac ggtttctctg gcgcatgcaa
     2821 tgcgtgctca ccctcatctt cttatggagg atttaaggat tctggttatt gaccttgatc
     2881 cgcaatcttc agcaacgatg tttttaagcc ataaacactc tattggtatc gtaaacgcaa
     2941 catctgcaca ggctatgttg cagaatgtaa gccgtgaaga gctgttagag gagtttattg
     3001 ttccttctgt tgtacctggg gttgacgtta tgcctgcgtc gattgacgat gcctttattg
     3061 catccgattg gagagagctg tgcaatgagc atctaccggg tcagaacatc catgctgtcc
     3121 tgaaagaaaa tgtgattgat aagctgaaga gcgattatga ctttatcctc gttgatagtg
     3181 gtcctcacct tgacgccttc ctgaaaaatg ctttggcctc ggccaatata ctgtttacac
     3241 ctctgccgcc agcaactgtc gatttccact catcgcttaa atacgttgcc cgccttcctg
     3301 agttggtgaa actcatttcg gatgaaggct gcgagtgcca gcttgcgact aacattggtt
     3361 ttatgtccaa gttgagtaac aaggcagacc ataagtattg ccatagcctg gctaaagaag
     3421 tgttcggtgg ggatatgctt gatgtcttcc tccctcgcct tgacggtttt gaacgctgcg
     3481 gcgagtcttt tgacactgtt atttcagcta acccggcaac gtatgttggt agtgctgatg
     3541 cattgaagaa cgcgcgaatt gccgcggaag attttgctaa agcagttttt gaccgtattg
     3601 aatttatcag atctaactga ggagtaagaa acccccatgt caaagaaaaa cagaccaaca
     3661 attgggcgaa cccttaatcc ttcaatatta agcggatttg atagttcttc agcctctggc
     3721 gatcgagtcg agcaggtatt caagttatca actggtcgcc aggccacatt tattgaagag
     3781 gtaatacctc cgaaccaggt agaaagcgat acctttgttg atcagcataa caacgggcgt
     3841 gaccaggcat ctcttacgcc aaaatcatta aaaagtatcc gaagcactat taagcatcag
     3901 caattttacc ctgcaatagg tgttagacgg gctacaggga aaattgaaat tttggatggt
     3961 tcccggcgtc gagcttctgc catcttagag aacgtagggt tgcgggtttt agtcacggac
     4021 caggagatca gcgttcagga agcgcaaaat ttagcgaaag acgttcagac agcattgcag
     4081 cacagcattc gagaaatagg tctgcgtttg atgcgaatga aaaatgatgg gatgagtcag
     4141 aaggatattg cagccaaaga agggctgtct caggcgaagg tcacgcgtgc tctccaggca
     4201 gcgagtgctc cggaagaatt agtcgccctt ttccctgtgc agtcggaatt aaccttttcg
     4261 gactacaaaa cgctttgtgc tgttggcgac gaaatgggga acaagaattt agagtttgat
     4321 cagcttattc aaaacatatc cccggaaata aacgacatct tatccattga agaaatggcc
     4381 gaagatgaag ttaaaaataa aatcctgcgc ttgataacaa aggaagcctc actactcacg
     4441 gataaaggtt ctaaagataa gtccgtagtt actgaattat ggaaatttga ggacaaggat
     4501 cgctttgcaa ggaagcgcgt gaaaggccgt gcattttctt atgagtttaa tcgactctca
     4561 aaagagttac aggaagaact cgacaggatg attgggcata tccttagaaa gagcctcgat
     4621 aaaaagccga agccttaaac tttcgccatt caaatttcac tattaactga ctgtttttaa
     4681 agtaaattac tctaaaattt caaggtgaaa tcgccacgat ttcaccttgg attttacctt
     4741 cctcccctcc tcccgaaaaa aataaaaaaa ttgcttgtca cgagaaagtc aacaagtgac
     4801 tttcaataaa atctcttccg aaaagggatt cacacaagtg cccttgtgtt taaggaagag
     4861 taaattgagt aacttacgcg aataccagaa tcgtattgca gatatcgcaa aacgctctaa
     4921 agctgtgctt ggctgggcaa gcactgcgca gttcggtact gataaccaat tcattaaaga
     4981 tgatgccgcg cgtgccgcat ctatccttga agctgcacgt aaagacccgg tttttgcggg
     5041 tatctctgat aatgccaccg ctcaaatcgc tacagcgtgg gcaagtgcac tggctgacta
     5101 cgccgcagca cataaatcta tgccgcgtcc ggaaattctg gcctcctgcc accagacgct
     5161 ggaaaactgc ctgatagagt ccacccgcaa tagcatggat gccactaata aagcgatgct
     5221 ggaatctgtc gcagcagaga tgatgagcgt ttctgacggt gttatgcgtc tgcctttatt
     5281 cctcgcgatg atcctgcctg ttcagttggg ggcagctacc gctgatgcgt gtaccttcat
     5341 tccggttacg cgtgaccagt ccgacatcta tgaagtcttt aacgtggcag gttcatcgtt
     5401 ttggttctta tgctgctggt gatgttctgg acatgcaatc cgtcggtgtg tacagccagt
     5461 tacgtcgccg ctatgtgctg gtggcaagct ccgatggcac cagcaaaacc gcaaccttca
     5521 agatggaaga cttcgaaggc cagaatgtac caatccgaaa aggtcgcact aacatctacg
     5581 ttaaccgtat taagtctgtt gttgataacg gttccggcag cctacttcac tcgtttacta
     5641 atgctgctgg tgagcaaatc actgttacct gctctctgaa ctacaacatt ggtcagattg
     5701 ccctgtcgtt ctccaaagcg ccggataaag gcactgagat cgcaattgag acggaaatca
     5761 atattgaagc cgctcctgag ctgatcccgc tgatcaacca cgaaatgaag aaatacaccc
     5821 tgttcccaag tcagttcgtt atcgcggctg agcacacggt acaggcggcg tatgaagcac
     5881 agcgtgaatt tggtctggac ctgggttccc tacagttccg caccctgaag gaatacctgt
     5941 ctcatgaaca ggatatgctg cgtcttcgca tcatgatctg gcgcactctt gcgaccgaca
     6001 cctttgacat cgctctgccg gtt
//