From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: E. coli hemA and hemM genes involved in 5-aminolevulinic acid
Date: 12 Jan 93 05:46:07 GMT
Distribution: bionet
Checksum: 51096 5

LOCUS       ECOALVB      1955 bp ds-DNA             BCT       12-JAN-1993
DEFINITION  E. coli hemA and hemM genes involved in 5-aminolevulinic acid
            synthesis.
ACCESSION   D10264
KEYWORDS    5-aminolevulinate synthase; delta-aminolevulinate synthase.
SOURCE      E. coli (strain C600) genomic DNA.
  ORGANISM  Escherichia coli
            Prokaryotae; Bacteria; Gracilicutes; Scotobacteria; Facultatively
            anaerobic rods; Enterobacteriaceae.
REFERENCE   1  (bases 1 to 1955)
  AUTHORS   Ikemi,M., Murakami,K., Hashimoto,M. and Murooka,Y.
  TITLE     Cloning and characterization of genes involved in the biosynthesis
            of delta-aminolevulinic acid in Escherichia coli
  JOURNAL   Gene 121, 127-132 (1992)
  STANDARD  full automatic
COMMENT     Submitted (20-Jan-1992) to DDBJ by: Yoshikatsu Murooka Department
            of Fermentation Technology Faculty of Engineering Hiroshima
            University 1-4-1 Kagamiyama Higashi-Hiroshima 724 Japan Phone:
            0824-22-7111 Fax:    0824-22-7191.
FEATURES             Location/Qualifiers
     RBS             345..348
     CDS             complement(<1..146)
                     /gene="hemA"
                     /product="delta-aminolevulinate synthase"
                     /codon_start=1
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLST"
     CDS             360..983
                     /gene="hemM"
                     /product="5-aminolevulinate synthase"
                     /codon_start=1
                     /translation="MPLPDFRLIRLVPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     CDS             1159..1770
                     /product="ORF3"
                     /codon_start=1
                     /translation="VEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGGG
                     LGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEILT
                     PVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFTQAYGRANTKGA
                     PFRRTAVKCRSLGKLLLECAGKCLLRVEAVLQRDVQNRTRSQA"
BASE COUNT      496 a    490 c    530 g    439 t
ORIGIN      27 min on E. coli chromosome
        1 gtcgacagca ccacgccgcc ctgcaccatc ggctgcgcaa gcaggctgtc aagcgcctga
       61 tcgagcttat ccggcgaaaa cgatacacgt tctcgcagcg atacaggtgc cgttttatgg
      121 ttgataccga gtgctaaaag ggtcatgtct gcgggaaata ataccaacgt tgatagggtt
      181 agtctgcttg catcatacag gatgcgtaag atcaataaaa gagagcgccc ccttttggag
      241 taattgccga aagccgttag attctggcaa ttaagacaac ttgaacatag acgatagcgg
      301 acggtaacgc tagcattaag ggttataact gcaacgtatc tcaaggactt gtcatcacta
      361 tgcccctgcc cgattttcgt cttatccgcc tcgtaccgct ggctgctctt gtgctcactg
      421 cctgttccgt taccacgccc aaaggtcctg gcaaaagccc ggattcgcca caatggcgtc
      481 agcatcagca agacgtgcgc aatcttaatc agtatcagac tcgcggcgcg ttcgcttata
      541 tttctgacca acaaaaagtg tacgcccgct ttttctggca gcaaaccggc caggatcgct
      601 accgtctgct gctcactaac ccattgggca gcacggaact ggagctgaat gctcaaccgg
      661 gtaacgtgca gttagtcgac aataaaggtc agcgttatac cgccgatgac gccgaagaga
      721 tgattggcaa attgaccgga atgccaattc cgctcaacag cttgcgccag tggattttag
      781 gtttaccggg tgatgcaacc gactacaaac tggacgacca gtaccgcctg agcgaaatta
      841 cctacagcca gaatggcaaa aactggaagg ttgtttatgg tggttatgac accaaaacgc
      901 aacctgcgat gccagccaat atggaactca ccgacggtgg tcaacgcatc aagttaaaaa
      961 tggataactg gatagtgaaa taatgcggac acagtggccc tctccggcaa aacttaatct
     1021 gtttttatac attaccggtc agcgtgcgga tggttaccac acgctgcaaa cgctgtttca
     1081 gtttcttgat tacggcgaca ccatcagcat tgagcttcgt gacgatggga tattcgtctg
     1141 ttaacgcccg ttgaaggcgt ggaacatgaa gataacctga tcgttcgcgc agcgcgattg
     1201 ttgatgaaaa ctgcggcaga cagcgggcgt cttccgacgg gaagcggtgc gaatatcagc
     1261 attgacaagc gtttgccgat gggcggcggt ctcggcggtg gttcatccaa tgccgcgacg
     1321 gtcctggtgg cattaaatca tctctggcaa tgcgggctaa gcatggatga gctggcggaa
     1381 atggggctga cgctgggcgc agatgttcct gtctttgttc gggggcatgc cgcgtttgcc
     1441 gaaggcgttg gtgaaatact aacgccggtg gatccgccag agaagtggta tctggtggcg
     1501 caccctggtg taagtattcc gactccggtg atttttaaag atcctgaact cccgcgcaat
     1561 acgccaaaaa ggtcaataga aacgttgcta aaatgtgaat tcacccaggc gtacgggcgg
     1621 gcgaatacca aaggcgctcc attcaggaga acggcggtaa agtgcaggag tctgggcaaa
     1681 ctgcttcttg aatgcgcggg taaatgtctg ttgagagtcg aagcggtatt gcagcgcgat
     1741 gtccagaatc ggacgcgcag tcaggcgtag tgcgaccgcc gatttcgaca aacgacgagc
     1801 acgaatatac gcgccaatag catggccagt gacatcttta aacattctct gtaagtgcca
     1861 cttggaataa cctgcttcgc cgctacattg tcgagcgaca ggggctgatc cagatgacct
     1921 tccagcctga ttaaaaggtc gcgaataatg ccggc
//