From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Vibrio parahaemolyticus lateral flagellin (lafA) gene, complete
Date: 29 Dec 92 20:59:07 GMT
Distribution: bionet
Checksum: 00381 8

LOCUS       VIBLAFAB     3724 bp ds-DNA   Circular  BCT       11-DEC-1992
DEFINITION  Vibrio parahaemolyticus lateral flagellin (lafA) gene, complete
            cds; hook-associated protein type 2 (lafB) gene, complete cds.
ACCESSION   L06176
KEYWORDS    flagellin; hook-associated protein type 2; lafA gene; lafB gene.
SOURCE      Vibrio parahaemolyticus (strain BB22) (library: pLAFRII) DNA.
  ORGANISM  Vibrio parahaemolyticus
            Prokaryotae; Facultatively anaerobic gram-negative rods;
            Vibrionaceae.
REFERENCE   1  (bases 1 to 3724)
  AUTHORS   McCarter,L.L. and Wright,M.E.
  TITLE     Identification of genes encoding components of the swarmer cell
            flagellar motor, propeller, and a sigma factor controlling
            differentiation of Vibrio parahaemolyticus
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     stem_loop       460..488
     stem_loop       599..628
     RBS             726..732
                     /gene="lafA"
     terminator      1637..1661
     stem_loop       2133..2164
     RBS             2358..2361
                     /gene="lafB"
     CDS             739..1593
                     /standard_name="lateral flagellin"
                     /gene="lafA"
                     /product="flagellin"
                     /codon_start=1
                     /translation="MALSMHTNYASLVTQNTLNSTSGLLNTAMERLSTGYRINSASDD
                     AAGLQIANRLEAQTRGMSVAMRNAQDGISMMQTAEGAMEEMTNITYRMNDLATQSLNG
                     SNSDKDRAAMDAEFKQLSAELNNIMGNTSFGGQKLLAAGGGFEAGAVTFQIGASSAET
                     LDVDASASIKKVAATLADAAITDGIGDATKAKAALDKISDAGGLIEDIGATRAQFGAN
                     INRLEHTMTNLGNMVENTSAAKGRIMEADFAVESSNMTKNQMLMQAGTTVLSKTNQLP
                     SMAMSLLR"
     CDS             2369..3706
                     /gene="lafB"
                     /product="hook-associated protein type 2"
                     /codon_start=1
                     /translation="MSSIDPATFAAQFAQIEIQPFKQRYQLQTNTYQSQLSALGKVES
                     AMREFRTALNEMNSSTNSIIKNSTSISQEGYFTANADAKALSGSYQIFVEQVATSHQV
                     STGMPADLDATTEIPKTGNLEFTINGKTMTIDLSTVDTDGDGVTTVSDLTKAINNNSD
                     NPGVNATLVRSNGQTHFMLSSTETGVANQINVSATGTGQAWFEDAFTNLSQISAPQDA
                     VIWLGAENTGLKLTNSSNTFEGVIDGVDITVTKAQTSGETAIGLGIGADDEATKEQLN
                     KFVDAYNTLISTIDEHTQIGSEDKKRGVLASDPTMRSIESQLSSLVRGEHGGMRLSEI
                     GVTLDRHGKLKVDQEKFAEAQKNNSAGLEAMFNGDGALLDSMDAMAEPFLKFSSGAFK
                     SRKEALQANLDRLSDKQTTLERKYDMSYKRYLKQFTQMNTLMTQMNQTMSMFG"
BASE COUNT     1165 a    848 c    753 g    958 t
ORIGIN      
        1 ggatccgggc gtgtattttc ttgagcagat ggttcagttt cactcagccg cttttcagca
       61 tgtaatccca tccttacaag cagatgaaac attgtgcctt cacgaacgca tggaaacgct
      121 tgtcgaaaaa ctgaaggaaa atgcgtttgc attgtggttt gaagtagcca catcccaaac
      181 gcattttgag cgtattgctg agtttgatcc agatatgatc aaactctcgg taacgataga
      241 gaacaaacaa gatcaacact catttttacc cattgcgcga tttctaagaa aacacaaata
      301 ccagtgggtt gctggtcgcg ttgcaagcca agtcgaactc aaccgctatc gactgctcgg
      361 tgcaagttat tactttggtt acttcagtga catccctact tccctgtctt tccgttcctt
      421 tgatgaggaa tagctgaaac ccaaccctca tggctaaact aaaaagcgcg aatgattcgc
      481 ggctttttta cttttctcgt tttagatttt ctctttgcct taatcttatg ccgatcaaaa
      541 cacatcggaa actaacgcta aaacggtaac aaaccccaac ttagtggaat gcaagtcact
      601 aaaaatcatc aattttgatc ttttttagaa aatttttaat tttcaaatta atcattaatg
      661 gcaagcgacc gcctctagtt agtgactagg gaatatccca aggcctgaaa gaaatagtga
      721 taaactaagg agactaagat ggctttatca atgcacacta actacgcatc acttgtaact
      781 caaaacactc taaacagcac aagtggtctt ttaaatactg cgatggagcg tctaagcacc
      841 ggttaccgca tcaactcagc ttctgatgac gctgcaggtc tacaaattgc taaccgtttg
      901 gaagctcaaa ctcgcggcat gtctgtagca atgcgtaacg ctcaggacgg catctctatg
      961 atgcaaactg ctgagggtgc aatggaagag atgactaaca tcacttaccg tatgaacgac
     1021 cttgcaacac aatctttgaa cggttcaaac agtgataaag accgcgcagc aatggacgct
     1081 gagttcaaac agctttcagc tgaactaaac aacatcatgg gcaacacatc ttttggtggt
     1141 caaaaactac tagcagctgg tggtggtttt gaagcgggcg cagttacttt ccaaatcggt
     1201 gcttcttcag ctgaaactct agacgtagac gcatctgcaa gcatcaaaaa agtagcagct
     1261 acacttgctg acgcagctat cactgacggt atcggtgacg caactaaagc taaagcagct
     1321 cttgataaaa tcagtgatgc tggtggcctt atcgaagata tcggtgctac tcgtgcgcag
     1381 tttggtgcaa acatcaaccg tctagagcac acaatgacta acctaggtaa catggttgag
     1441 aacacttctg cagctaaagg ccgtatcatg gaagcagact tcgcagttga gtcttcaaac
     1501 atgactaaga accaaatgct tatgcaagct ggtactacag ttctttctaa aactaaccag
     1561 ctacctagta tggcaatgtc tctacttcgt taataacgaa ttagaacgta cgatgttgtt
     1621 aatacgtaca aaaagcaaaa gcctccctat gggaggcttt tttatcacct acgtatataa
     1681 atagagcttg tattcccgca ttgttcttca ggaaccctaa agcctatttt tcgcatcatg
     1741 ttgtgaagta ctttgttaaa tagctccaat tcaactgtct cgcttccctg tccttgttga
     1801 taagttttta actcagggaa agtggatatc gcttcctgct ggatactggc caattcatcc
     1861 gcattaatat tttgtttgct cagtaactta gcactaataa atgtctcgta agatcgagcg
     1921 catttcatta gtcgtaaaac aaattcattc atttctgtcg ttcttttcta agtgatagcc
     1981 tgacgttggt aaggtgcgct aaaacaccaa aatatgccga gttaaaagat accaaaaatc
     2041 gatgtacttc ctctgtagga atagtcctat ttacatagca ggaaacacct aaagcagcgc
     2101 tttattaacg gcttaatcaa cacgtcacat tcctaaaatg aattaaatat tttccgcttt
     2161 ttagtcgcct tgtcttatag ggaaacacta gatgttttcg agaggaaggc ggaacacata
     2221 aggtggaaaa tctacttcct ctcaaattta aatagtatcg caagtcaatg ttttataaat
     2281 acatttaagt tcggcacgta tcttgcgata agacatactt tcaaggcata gagttacgcc
     2341 aattaccaat gagagaggga gtaagctcgt gagttcaata gatccagcaa cctttgcagc
     2401 ccagttcgcg cagatcgaaa ttcaaccttt taaacaacgc taccaactgc aaaccaatac
     2461 gtatcaatct cagctatctg ctctgggtaa agttgagagt gccatgcgag agttccgcac
     2521 tgcgctcaac gaaatgaaca gcagcacaaa cagcatcatt aaaaactcca cctccatctc
     2581 tcaagaaggc tactttactg caaatgcaga cgctaaagct ctatcgggta gttatcagat
     2641 attcgtcgag caagtagcaa cgtcacacca agtttctact ggaatgccag cagatctaga
     2701 cgcaaccact gaaatcccaa aaacaggaaa tcttgagttc accatcaacg gtaaaacaat
     2761 gacgattgat ctatcaacgg tcgataccga cggtgatggt gtaacgacgg tgagtgacct
     2821 aacgaaagcg atcaataaca acagtgataa cccaggtgta aacgcaacct tggtacgctc
     2881 aaatggtcag actcacttca tgctttcaag cactgaaacc ggcgtcgcta accaaatcaa
     2941 cgtgtccgca acgggcacag gccaagcatg gtttgaagat gcattcacaa accttagtca
     3001 aatcagtgcc ccccaagatg ccgtgatctg gttgggtgca gagaatactg gtctaaaact
     3061 cactaactcg agcaatacgt ttgaaggtgt tatcgatggc gtagacatta ccgtaaccaa
     3121 ggctcagacc tctggagaaa ccgcgattgg tttaggtatc ggcgctgatg acgaagcaac
     3181 caaagagcag cttaacaagt tcgtagacgc ctacaacaca ttgatatcta caattgatga
     3241 acacacacaa atcggcagtg aagataaaaa acgcggtgtg ctagctagcg acccaaccat
     3301 gcgctcaatt gaaagccaac tatcaagcct tgttcgaggc gagcacggcg gcatgcgctt
     3361 aagcgaaata ggcgtaacgt tggatcgcca tggcaaactg aaagtggacc aagaaaaatt
     3421 tgcagaggca caaaaaaaca acagcgcagg cttagaagca atgttcaatg gcgacggtgc
     3481 tttacttgat tcaatggacg ccatggccga accattcctc aaattttctt ctggtgcgtt
     3541 caaatctcgt aaagaagcgc tgcaagcaaa cttagatcgc ctctcagaca aacaaaccac
     3601 tttagaacgt aagtacgaca tgtcgtacaa gcgttaccta aaacaattca ctcaaatgaa
     3661 tacattaatg acccaaatga accaaaccat gtcaatgttt ggctaaggct ttaggagaag
     3721 atct
//