From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: D.pseudoobscura Adh gene for alcohol dehydrogenase
Date: 28 Dec 92 21:27:41 GMT
Distribution: bionet
Checksum: 05275 8

LOCUS       DROADHGN61   3498 bp ds-DNA             INV       01-DEC-1992
DEFINITION  D.pseudoobscura Adh gene for alcohol dehydrogenase
ACCESSION   X64470
KEYWORDS    ADH gene; alcohol dehydrogenase.
SOURCE      Drosophila pseudoobscura DNA.
  ORGANISM  Drosophila pseudoobscura
            Eukaryota; Animalia; Metazoa; Arthropoda; Uniramia; Insecta;
            Pterygota; Neoptera; Holometabola; Diptera; Brachycera;
            Cyclorrhapha; Schizophora; Drosophiloidea; Drosophilidae.
REFERENCE   1  (bases 1 to 3498)
  AUTHORS   Schaeffer,S.W. and Miller,E.L.
  TITLE     Estimates of gene flow in Drosophila pseudoobscura determined from
            nucleotide sequence analysis of the alcohol dehydrogenase region
  JOURNAL   Genetics 132, 471-480 (1992)
  STANDARD  full automatic
COMMENT     *source: strain=PS170; *source: isolate=Stemwinder Provincial Park,
            B.C. Canada; *source: chromosome=IV section 88;
               From EMBL    entry DPADHGN61;  dated 16-OCT-1992.
FEATURES             Location/Qualifiers
     mRNA            join(40..119,915..1020,1083..1487,1548..1981)
                     /note="adult mRNA (Adh)"
     mRNA            join(882..1020,1083..1487,1548..1981)
                     /note="larval mRNA (Adh)"
     promoter        8..14
                     /note="adult promoter (Adh)"
     prim_transcript 40..1981
                     /note="adult primary transcript (Adh)"
     exon            40..119
                     /note="adult exon 1a"
     intron          120..914
                     /note="adult intron"
     prim_transcript 882..1981
                     /note="larval primary transcript (Adh)"
     exon            882..1020
                     /note="larval exon 1"
     exon            915..1020
                     /note="adult exon 1b"
     intron          1021..1082
                     /number=1
     exon            1083..1487
                     /number=2
     intron          1488..1547
                     /number=2
     exon            1548..1981
                     /number=3
     polyA_signal    1958..1963
     polyA_site      1981
     CDS             join(928..1020,1083..1487,1548..1814)
                     /EC_number="1.1.1.1"
                     /gene="Adh"
                     /product="alcoholdehydrogenase"
                     /codon_start=1
                     /translation="MSLTNKNVVFVAGLGGIGLDTSRELVKRNLKNLVILDRIDNPAA
                     IAELKAINPKVTITFYPYDVTVPVAETTKLLKTIFAQVKTIDVLINGAGILDDHQIER
                     TIAVNYTGLVNTTTAILDFWDKRKGGPGGIICNIGSVTGFNAIYQVPVYSGSKAAVVN
                     FTSSLAKLAPITGVTAYTVNPGITKTTLVHKFNSWLDVEPRVAEKLLEHPTQTSQQCA
                     ENFVKAIELNKNGAIWKLDLGTLEPITWTQHWDSGI"
BASE COUNT      975 a    795 c    803 g    925 t
ORIGIN      
        1 cgtagtgtat ttaaggcact gcacatgtcg aggaccacga ttattgtcta cagtgcagtc
       61 agtagttgtc aggtgcagca ggcggaccag catttcttta aatctactca atcgatcaag
      121 taagtaactg taatccaagt acagtcggcc catcccactc ggttctgccg taactcaaga
      181 aatttcttga tagcgcagtt ccagttgtag aacgtcgtct aaaaaaacat ctcgttggag
      241 cttgggttga aaaacgttcg agttgggcgt agaaaatttc aaatataaaa tatcaaaaaa
      301 gagaaaatat cttcccaaag cagctgtcca ttttttgtta aagtgataga tcaaaaattc
      361 gtatgacgta tttgccaagt gctctttatg caaataattt cctgaattct aattctacaa
      421 gaagcacttt tggctcttgt ccagaaaaaa caatttgttt acacttgtcg gttgataaca
      481 gaggcggacc gacggctaag taacataaca gtcgcctgtt aagtaatgcc ctcactctct
      541 gttagatggc cgcgcgtgtc gactccgata gaccagcaga cacgcagtag ttttcctcaa
      601 gaacctgaac tctaaacata gacataattt acgatacctt gagactcaaa taaaaataca
      661 agtatgagtg gtattctatt cctgattaga ctcgcacaca cacacgtacg agtattaaca
      721 taaatttgcc ggcaaataac caaaacaaaa cgaacgcaaa cgatccgaac acgtaatgcg
      781 agagataaga aacagaaaag ctcgacgtga aagaagcttc tggcggaagc tcttgccgaa
      841 ctccctataa atacgagaca gaaaaccaga ataaatataa taacaaactg ttgaattgtc
      901 ccatcgattc ccagatcaaa agacagaatg tctctcacca acaagaacgt cgttttcgtg
      961 gccggtctgg gcggcattgg cctggacacc agccgggagt tggtcaagcg taatctgaag
     1021 gtaagagaga aagaattcca tggaatctat ggaatcctaa attaaaaatt tatatttttt
     1081 agaacctggt catcctggat cgcattgaca atccggctgc cattgccgaa ctgaaggcaa
     1141 tcaatcccaa ggtgaccatc accttctatc cctacgatgt gactgtgccc gtcgctgaga
     1201 ccaccaagct cctgaagacc atctttgccc aggtgaagac aatcgatgtc ctgatcaacg
     1261 gtgctggcat cctggacgat catcagatcg agcgcaccat tgccgttaac tacacgggcc
     1321 tggtcaacac caccacagcc attctggact tctgggacaa gcgcaagggc ggcccaggcg
     1381 gcatcatttg caacattggc tccgtcaccg gtttcaatgc catctaccag gtgcccgttt
     1441 actccggctc caaggcggcg gtggttaact ttacctcctc cctggcggta agtaatccaa
     1501 cctttttcct actccccaaa actaaatatt tctttgaaat cccatagaaa ctggctccca
     1561 ttactggtgt cactgcttac actgtcaatc ctggcatcac taagaccact ctggtccaca
     1621 aattcaactc gtggctggat gtggagcccc gtgtggcgga gaagctgctc gagcatccca
     1681 cccagacctc tcagcagtgc gccgagaact ttgtgaaggc cattgagctg aacaagaacg
     1741 gtgccatctg gaagttggat ttgggcacct tggagcccat cacatggacc cagcactggg
     1801 actcgggcat ctaaaccatc ccagagactc tatgggacat ggcgttttag cttttagttt
     1861 ctttttttcc actcaattgt tacgtatata catacacata tggcaataag gctgatttga
     1921 ttctctttta tggaaggaac cccgttttga atatgataat acaaaattat atttgagaaa
     1981 tttcaacaaa atcgataaga gctatagctc tcttttaatt aaaaaaaaac tagataaaaa
     2041 gagcaatggt cagtggtggt cagtggtcgt tgtggcctct atcgatttcc acacaaaaac
     2101 tttactgttt agtaatagaa aagaacttga gaaaggcagc caaaagaatg tacgatctga
     2161 cgggtaagca tgtctgctat gtggctgact gcggtggcat tgcactggag accagcaagg
     2221 ttctcatgac caagaatata gcggtgagtg gtagagtggt gtagagagtg gagagtggtt
     2281 ctggttctta aacaagtctc tagtctctag tctctagtct ctagtctctt ggctgctgct
     2341 ggtagctgtc gaaactaatt aagttaatga ctttttcatg ttttgttttt gtccgctgac
     2401 ttttgctgtt caaagctaca attaagttta tgttttgagt ttgatctact tttgcttaag
     2461 ggaagtactt tcgattacgg attgctggca tataaaaata gataccaagg aatgtgcctc
     2521 atatcatatt gattctatct tgattctttg cccagaaact ggccatcctt cagagcgtgg
     2581 aaaacccccc ggccatcgcc cagttacaat cgattaagca cagcacacag atcttcttct
     2641 ggaccttcga tgtgaccatg gcccgcgagg agatgaagaa gtacttcgat gaggtcatgg
     2701 tccagatgga ctacatcgat gtgctgatca atggggccac cctgtgcgat gagcggaaca
     2761 ttgatgccac catcaatacg aatctgaccg gcatgatgaa caccgtggcc actgtgctgc
     2821 cctatatgga ccgtaagatg ggcggttcgg gtggactgat tgtgaatgtc acctccgtca
     2881 taggactgga tccatcgcca gtcttttgtg cgtacagtgc ctcaaagttt ggtgtgattg
     2941 ggttcacccg aagtctagcg gtgagtgggc gagatcttag cttgaaaatg ctctctaatg
     3001 tcttccattc tttcgcagga tcccctgtat tacacccaaa atggtgtggc tgtcatggcc
     3061 gtctgctgtg gtcccaccaa agtgtttgtc gatcgcgaac tgactgcctt cctggaatac
     3121 ggccagacct ttgccgatcg cctgcgtcgt gccccctgcc aatcgacggc cgtctgcggc
     3181 aagaatatag taaatgccat cgagagatcg gagaacggac agatctggat tgccgacaag
     3241 ggcggcctgg agtcggtggc ccttcactgg tattggcata tggccgatca gtttgtgaac
     3301 tacatgcaga gcactgacga tgaggatcag gagttttttt tggggcagcg atgaggcatg
     3361 ccttgatcca cggaataaaa catcaaatgt gtcacaaaaa gtaggcaact tgtttgtggg
     3421 tctggaaata gatttgaaat attttgaggt catatgaata atgacataaa tgagggtgcc
     3481 tgaaagtatg ccaagttt
//