From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: D.pseudoobscura Adh gene for alcohol dehydrogenase
Date: 28 Dec 92 21:27:57 GMT
Distribution: bionet
Checksum: 39336 8

LOCUS       DROADHGN71   3470 bp ds-DNA             INV       01-DEC-1992
DEFINITION  D.pseudoobscura Adh gene for alcohol dehydrogenase
ACCESSION   X64480
KEYWORDS    ADH gene; alcohol dehydrogenase.
SOURCE      Drosophila pseudoobscura DNA.
  ORGANISM  Drosophila pseudoobscura
            Eukaryota; Animalia; Metazoa; Arthropoda; Uniramia; Insecta;
            Pterygota; Neoptera; Holometabola; Diptera; Brachycera;
            Cyclorrhapha; Schizophora; Drosophiloidea; Drosophilidae.
REFERENCE   1  (bases 1 to 3470)
  AUTHORS   Schaeffer,S.W. and Miller,E.L.
  TITLE     Estimates of gene flow in Drosophila pseudoobscura determined from
            nucleotide sequence analysis of the alcohol dehydrogenase region
  JOURNAL   Genetics 132, 471-480 (1992)
  STANDARD  full automatic
COMMENT     *source: strain=PS262; *source: isolate=Kiabab National Forest,
            Arizona; *source: chromosome=IV section 88;
               From EMBL    entry DPADHGN71;  dated 16-OCT-1992.
FEATURES             Location/Qualifiers
     mRNA            join(40..119,921..1026,1091..1495,1556..1971)
                     /note="adult mRNA (Adh)"
     mRNA            join(888..1026,1091..1495,1556..1971)
                     /note="larval mRNA (Adh)"
     promoter        8..14
                     /note="adult promoter (Adh)"
     prim_transcript 40..1971
                     /note="adult primary transcript (Adh)"
     exon            40..119
                     /note="adult exon 1a"
     intron          120..920
                     /note="adult intron"
     prim_transcript 888..1971
                     /note="larval primary transcript (Adh)"
     exon            888..1026
                     /note="larval exon 1"
     exon            921..1026
                     /note="adult exon 1b"
     intron          1027..1090
                     /number=1
     exon            1091..1495
                     /number=2
     intron          1496..1555
                     /number=2
     exon            1556..1971
                     /number=3
     polyA_signal    1948..1953
     polyA_site      1971
     CDS             join(934..1026,1091..1495,1556..1822)
                     /EC_number="1.1.1.1"
                     /gene="Adh"
                     /product="alcoholdehydrogenase"
                     /codon_start=1
                     /translation="MSLTNKNVVFVAGLGGIGLDTSRELVKRNLKNLVILDRIDNPAA
                     IAELKAINPKVTITFYPYDVTVPVAETTKLLKTIFAQVKTIDVLINGAGILDDHQIER
                     TIAVNYTGLVNTTTAILDFWDKRKGGPGGIICNIGSVTGFNAIYQVPVYSGSKAAVVN
                     FTSSLAKLAPITGVTAYTVNPGITKTTLVHKFNSWLDVEPRVAEKLLEHPTQTSQQCA
                     ENFVKAIELNKNGAIWKLDLGTLEPITWTQHWDSGI"
BASE COUNT      973 a    792 c    791 g    914 t
ORIGIN      
        1 cgtagtgtat ttaaggcact gcacatgtcg aggaccacga ttattgtcta cagtgcagtc
       61 agtagttgtc aggtgcagca ggcggaccag catttcttta aatctactca atcgatcaag
      121 taagtaactg taatccaagt acagtcggcc catcccactc ggtcactcgg ttctgcagta
      181 actcaagaaa tttcttgata gcgcagttcc agttgtaaaa cgtcgtttaa aaaaccatct
      241 cgttggagct tgggttgaaa aacgttcgag ttgggcgtag aaaatttcaa atataaaata
      301 tcaaaaaaga gaaaatatct tcccaaagca gctgtccatt ttttgttaaa gtgatagatc
      361 aaaaattcgt atgacgtatt tgccaagtgc tctttatgca aataatttcc tcaattctaa
      421 ttctacaaga agcacttttg gctcttgtcc agaaaaaaca atttgtttac acttgtcgga
      481 tgataacaga agcggaccga cggctaagta acataacagt cgcctgttaa gtaatgccct
      541 cactctctgt tagatggccg cgcgtgtcga ctccgataga ccagcagaca cgcagtagtt
      601 ttcctcaaga acctgaactc taaacataga cataatttac gataccttga gactcaaata
      661 aaaatacaag tatgagtggt attctattcc tgattagact cgcacacaca cgtacgagta
      721 ttaacataaa tttgccggca aataaccaaa acaaaacgaa cgcaaacgat ccgaacacgt
      781 aatgcgagag ataagaaaca gaaaagctcg acgtgaaaga agcttctggc ggaagctctt
      841 gccgaactcc ctataaatac gagacagaaa accagaataa atataataac aaactgttga
      901 attgtcccat cgattcccag atcaaaagac agaatgtctc tcaccaacaa gaacgtcgtt
      961 ttcgtggccg gtctgggcgg cattggcctg gacaccagcc gggagttggt caagcgtaat
     1021 ctgaaggtaa gagagaacga attccatgga atctatggaa atcctaaatt taaaaattta
     1081 ttttttttag aacctggtca tcctggatcg cattgacaat ccggctgcca ttgccgaact
     1141 gaaggcaatc aatcccaagg tgaccatcac cttctatccc tacgatgtga ctgtgcccgt
     1201 cgctgagacc accaagctcc tgaagaccat ctttgcccag gtgaagacaa tcgatgtcct
     1261 gatcaacggt gctggcatcc tggacgatca tcagatcgag cgcaccattg ccgttaacta
     1321 cacgggcctg gttaacacca ccacagccat tctggacttc tgggacaagc gcaagggcgg
     1381 cccaggcggc atcatttgca acattggctc cgtcaccggt ttcaatgcca tctaccaggt
     1441 gcccgtttac tccggctcca aggcggcggt ggttaacttt acctcctccc tggcggtaag
     1501 taatccaacg tttttcctac tccccaaaac taaatatttc tttgaaatcc catagaaact
     1561 ggctcccatt actggtgtca ctgcttacac tgtcaatcct ggcatcacta agaccactct
     1621 ggtccacaaa ttcaactcgt ggctggatgt ggagccccgt gtggcagaga agctgctcga
     1681 gcatcccacc cagacctctc agcagtgcgc cgagaacttt gtgaaggcca tcgagctgaa
     1741 caagaacggt gccatctgga agttggatct gggcaccttg gagcccatca catggaccca
     1801 gcactgggac tcgggcatct aaaccatccc agagactcta tgggacatgg cgttttagct
     1861 tttagtttct ttttttccac tcaattgtta cgtatataca tacacatatg gcaataaggc
     1921 tgatttgaac cccgttttga atatgataat acaaaattat atttgagaaa tttcaacaaa
     1981 atcgataaga gctatagctc tcttttaatt aaaaaaaact agataaaaag agcaatggtc
     2041 agtggtggtc agtggtcgtt gtggcctcta tcgatttcca cacaaaaact ttactgttta
     2101 gtaatagaaa agaacttgag aaaggcagcc aaaagaatgt acgatctgac gggtaagcat
     2161 gtctgctatg tggctgactg cggtggcatt gcactggaga ccagcaaggt tctcatgacc
     2221 aagaatatag cggtgagtgg tagagaggtg gtgtagaaag tggagagtgg ttctggttct
     2281 taaacaagtc tctagtctct tggctgctgc tggtagctgt cgaaactaat taagttaatg
     2341 actttttcat gttttgtttt tgtccgctga cttttgctgt tcaaagctac aattaagttt
     2401 atgttttgag tttgatctac ttttgcttaa gggaagtact ttcgattacg gattgctggc
     2461 atataaaaat agataccaag gaatgtgcat catatcatat tgattctatc ttgattcttt
     2521 gcccagaaac tggccatcct tcagagcgtg gaaaaccccc cggccatcgc tcagttacaa
     2581 tcgattaagc acagcacaca gatcttcttc tggaccttcg atgtgaccat ggcccgcgag
     2641 gagatgaaga agtacttcga tgaggtcatg gtccagatgg actacatcga tgtactgatc
     2701 aatggggcca ccctgtgcga tgagcggaac attgatgcca ccatcaatac gaatctgacc
     2761 ggcatgatga acaccgtggc cactgtgctg ccctatatgg accgtaagat gggcggttcg
     2821 ggtggactga ttgtgaatgt cacctccgtc ataggactgg atccatcgcc agtcttttgt
     2881 gcgtacagtg cctcaaagtt tggtgtgatt gggttcaccc gaagtctagc ggtgagtggg
     2941 cgagatctta acttgaaaat gctctctcat atcttccatt cttccgcagg atcccctgta
     3001 ttacacccaa aatggtgtgg ctgtcatggc cgtctgctgt ggtcccacca aagtgtttgt
     3061 cgatcgcgaa ctgactgcct tcctggaata cggccagtcc tttgccgatc gcctgcgtcg
     3121 tgccccctgc caatcgacgg ccgtctgcgg ccagaacata gtaaatgcca tcgagagatc
     3181 ggagaacgga cagatctgga ttgccgacaa gggcggcctg gagtcggtgg ccctccactg
     3241 gtattggcat atggccgatc agtttgtgaa ctatatgcag agcactgacg atgaggatca
     3301 ggagtttttt ttgggccagc gatgaggcat gccttgatcc acggaataaa acatcaaatg
     3361 tgtcacaaaa agtaggcaac ttgttttgtg tgtttggaaa tagatttgaa atattttgag
     3421 gtcatatgaa taatgacata aatgagggtg cctgaaagta tgccaagttt
//