From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: S.typhimurium alkyl hydroperoxide reductase (ahpC) and (ahpF)
Date: 27 Aug 92 10:01:00 GMT
Distribution: bionet
Checksum: 05526     7

LOCUS       STYAHPCFA    2609 bp ds-DNA             BCT       27-AUG-1992
DEFINITION  S.typhimurium alkyl hydroperoxide reductase (ahpC) and (ahpF)
            genes, complete cds's.
ACCESSION   J05478
KEYWORDS    alkyl hydroperoxide reductase; oxidoreductase.
SOURCE      S.typhimurium (strain TN1379) DNA.
  ORGANISM  Salmonella typhimurium
            Prokaryotae; Gram-negative facultatively anaerobic rods;
            Enterobacteriaceae.
REFERENCE   1  (bases 1 to 2609)
  AUTHORS   Tartaglia,L.A., Storz,G., Brodsky,M.H., Lai,A. and Ames,B.N.
  TITLE     Alkyl hydroperoxide reductase from Salmonella typhimurium: Sequence
            and homology to thioresoxin reductase and other flavoprotein
            disulfide oxidoreductases
  JOURNAL   J. Biol. Chem. 265, 10535-10540 (1990)
  STANDARD  full staff_entry
COMMENT     Draft entry and computer-readable sequence for [1] kindly submitted
            by L.Tartaglia, 05-JUN-1990.
FEATURES             Location/Qualifiers
     -35_signal      105..110
                     /note="ahpCF -35 region"
     -10_signal      129..134
                     /note="ahpCF -10 region"
     mRNA            142..>2608
                     /note="ahpCF mRNA"
     RBS             155..160
                     /gene="ahpC"
                     /note="putative"
     CDS             166..708
                     /standard_name="C22 protein"
                     /product="alkyl hydroperoxide reductase"
                     /gene="ahpC"
                     /codon_start=1
                     /translation="MSLINTKIKPFKNQAFKNGEFIEVTEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYSVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAA
                     HPGEVCPAKWKEGERLWLHP"
     RBS             959..964
                     /gene="ahpF"
                     /note="putative"
     CDS             970..2535
                     /standard_name="F52a protein"
                     /product="alkyl hydroperoxide reductase"
                     /gene="ahpF"
                     /codon_start=1
                     /translation="MLDTNMKTQLRAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNTLPVRKPSFLITNPGSQQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRDIDGDFEFETYYSLSCHNCPDVVQALNLMAVLNPRIKHTAIDGGTFQ
                     NEITERNVMGVPAVFVNGKEFGQGRMTLTEIVAKVDTGAEKRAAEALNKRDAYDVLIV
                     GSGPAGAAAAVYSARKGIRTGLMGERFGGQVLDTVDIENYISVPKTEGQKLAGALKAH
                     VSDYDVDVIDSQSASKLVPAATEGGLHQIETASGAVLKARSIIIATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKVRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGDIHSVALAGIFVQIG
                     LLPNTHWLEGALERNRMGEIIIDAKCETSVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKIA"
BASE COUNT      652 a    656 c    724 g    577 t
ORIGIN      13 minutes on S.typhimurium map.
        1 tcaggcggcc tttttacttt agatggctga cagggcgcag tgttcacaat gtcgcccgaa
       61 tcgggttgtt agttaacgct tattgatttg ataatggaaa cgcattagcc gaatcagcaa
      121 aatttcgtta acttactcct caacgaaaac acggaggaag tatagatgtc cttaattaac
      181 accaaaatca aacctttcaa aaaccaggcg ttcaaaaacg gtgaattcat tgaagtcacc
      241 gagaaagata ccgaaggccg ctggagcgtc ttcttcttct acccggccga ttttaccttt
      301 gtttgcccga ctgaactggg tgacgttgcc gaccattacg aagaactgca gaagctgggc
      361 gtagacgttt attccgtctc aaccgatact cacttcacgc acaaagcatg gcacagcagc
      421 tctgaaacta tcgcaaaaat caaatatgcg atgatcggcg acccgactgg cgccctgacc
      481 cgtaacttcg acaacatgcg tgaagatgaa ggtctggcag accgtgcgac ctttgttgtt
      541 gacccgcagg gtatcatcca ggcgatcgaa gttaccgctg aaggtatcgg ccgtgacgcg
      601 tctgacctgc tgcgtaaaat taaagcagca cagtacgttg ccgctcaccc aggcgaagta
      661 tgcccggcga aatggaaaga aggcgaacga ctctggctcc atccttagac ctggtcggta
      721 aaatctaaat ttccgtcgtc tttcacgcca tagctgcgtt ggcgtcgcct gctcaccccg
      781 gtcacttact tcagtaagct cccggggatt ctcaggctag ccgccttact ctgacgtgaa
      841 atactttgga aattgtttca gtcatgggcg cacccgcgcc catttcattt cagcaccatg
      901 acgcaagttg cattaatgca gtcccgcgaa gtcggcttgc atgatgatgt tttcagagag
      961 ggaagaagaa tgctcgacac aaatatgaaa acccagctca gggcttacct tgagaaactg
     1021 accaaacctg ttgagctgat tgccacgctg gatgacagcg ctaaatcggc ggaaatcaag
     1081 gaactgctgg ctgaaatcgc cgagctgtcc gacaaagtca cgtttaaaga agacaatact
     1141 ctgccggtgc gcaagccgtc tttcctgatt acgaatccag gttctcagca agggccgcgc
     1201 tttgccggtt ctccgctggg ccacgaattt acctcgctgg ttttggcgct gctgtggacc
     1261 ggcggtcatc cgtcaaaaga agcgcagtcg ctgctggagc agattcgcga tattgacggc
     1321 gattttgaat tcgaaactta ttattcactc tcctgccata actgcccgga cgtagtgcag
     1381 gcgctgaacc tgatggcggt gctgaacccg cgtattaaac atacggcaat tgatggcggc
     1441 accttccaga acgaaatcac cgaacgtaac gtgatgggcg ttccggcggt atttgttaac
     1501 ggtaaagagt tcggccaggg acgtatgact ctgaccgaga ttgtcgctaa agtggatact
     1561 ggcgcagaaa aacgcgcggc ggaagcgctg aacaaacgcg atgcgtatga cgtactgatt
     1621 gtcggctccg gcccggcggg cgcggcggcg gcggtctact cggcgcgcaa aggcatccgt
     1681 accggtctga tgggcgagcg cttcggcggt caggtgctgg ataccgtgga tatcgaaaac
     1741 tatatctcgg tgccgaaaac cgaaggccag aaactggcgg gcgcgctgaa agcgcatgtc
     1801 agcgattacg acgttgacgt tatcgacagc cagagcgcaa gtaaactggt tccggccgca
     1861 acagaaggcg gcttgcatca gattgaaacg gcgtccggcg cagtattgaa agcgcgtagt
     1921 atcatcatcg ccaccggcgc gaaatggcgc aatatgaatg tgccgggcga ggatcagtat
     1981 cgcaccaaag gcgtcaccta ttgtccgcac tgcgacggtc cgttatttaa agggaagcgt
     2041 gtggcggtca tcggcggcgg caactctggc gttgaagcgg ctatcgatct ggcaggtatt
     2101 gtagaacatg ttacgttgct ggagttcgcg ccggagatga aggcggatca ggtgctacag
     2161 gacaaagtgc gtagtctgaa aaatgtcgat atcattttga atgcgcaaac gacagaagtg
     2221 aagggcgacg gttcgaaagt ggtgggactg gagtatcgtg accgtgtcag cggcgatatt
     2281 catagcgtcg cgctggcagg gatttttgtg cagattggtc tgttgccgaa tacccactgg
     2341 ctggaaggcg cgcttgagcg taaccgcatg ggcgagatca ttattgacgc caaatgtgaa
     2401 accagcgtta agggcgtctt cgccgcaggc gattgcacca ccgttcctta caaacagatt
     2461 atcatcgcca cgggcgaagg ggcgaaagcc tcgttgagcg cctttgatta tctgattcgc
     2521 accaaaatcg cataaaaaga agtaagcaca cctgcaaggc cagttaactg gccatcgtaa
     2581 atggccgata gtgtaagcta ttcgggcct
//