From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Bacillus firmus resolvase (tnpR) gene, complete cds; transposase
Date: 9 Apr 92 10:01:58 GMT
Distribution: bionet
Checksum: 49742     8

LOCUS       BACTNPRAA    3251 bp ds-DNA             BCT       09-APR-1992
DEFINITION  Bacillus firmus resolvase (tnpR) gene, complete cds; transposase
            (tnpA) gene, putative cds.
ACCESSION   M90749
KEYWORDS    resolvase; tnpA gene; tnpR gene; transposase.
SOURCE      Bacillus firmus (strain OF4) DNA.
  ORGANISM  Bacillus firmus
            Prokaryota; Bacteria; Firmicutes; Endospore-forming rods and cocci;
            Bacillaceae.
REFERENCE   1  (bases 1 to 3251)
  AUTHORS   Ivey,D.M., Guffanti,A.A., Shen,Z., Kudyan,N. and Krulwich,T.A.
  TITLE     The cadC gene product of alkaliphilic Bacillus firmus OF4 partially
            restores Na+-resistance to an Escherichia coli strain lacking a
            Na+/H+ antiporter (NhaA)
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     RBS             135..140
                     /gene="tnpR"
     CDS             145..699
                     /function="site specific recombination during
                     transposition"
                     /standard_name="resolvase"
                     /product="TnpR"
                     /gene="tnpR"
                     /codon_start=1
                     /translation="MGKVFGYARVSTQDQILDLQIDVLEKAGAAVIYKEKITGTRKER
                     PELEQLLKAISKGDSVVVYKLDRISRSTKHLIELVETFEEKEVNFISIQDNIDTSTAM
                     GRFFFRTMASIAELERDIIVERTKSGLQSARMRGRNGGRPSKDPKLVERALKLHSSKQ
                     YSIKEITDMTGVSKSVLYRALEKN"
                     /citation=[1]
     RBS             716..721
                     /gene="tnpA"
     CDS             730..3251
                     /function="site-specific recombination"
                     /standard_name="transposase"
                     /note="putative coding sequence"
                     /product="TnpA"
                     /gene="tnpA"
                     /codon_start=1
                     /translation="MYLRARELLTPDQRKDFLLIPSTLSELGISVLTIPLHKHDIEVI
                     RRRRRDHNRLGFAIQICLFRYPGWSLSDIKNVPDKVINYVANQLQVDASEFKLYSERE
                     QTKHEHMEEIRKYYGFSNFSAQSYRIISQALLPHAIENSNALFLIRMTLEEMRKRKII
                     LPAMTTIERLVWETRRRAEEKVYNSLYKPLSPWQKQQLEKLIDTPSDKSKTKLGWLRE
                     IPGQSSPDAFLKVIERLEYVRLLNLSTESENIHSNRLLQLARLGARYEPHSFRRFNEN
                     KRYAILVAHLLTLSQDLIDQAIEIHDRQIMILQSKGRKAQEELQKQNGKSINEKVLHF
                     ADIGEALVKARNEELDPFEVLEKIMPWERIVDSIEEAKRLAHPMDYDYLDLLVTRFSY
                     LRKYTPVLLSKLEFRTTQASEPLLRALNVLREINHNKKRHIPEGAPLDFVPKRWQKHV
                     YDEDGNINRKYYELAALTELKNHIRSGDIWVAGSRLHKDCEEYLVTKDNWDETKNTGN
                     RLPVGISTQEYIIERNAALNKRLDYILENIDSLEGISIDKSRMRLDRLEKDTPEDAKS
                     LSQTLYNMLPRVKLTDLLIEVSNWTGFDEHLAHASSNRPPKGEEKSIVMATIMAMGTN
                     IGFTKMVEATPGITYHQLANAAQWRLHEDSLSKAQATLVNFQHLSLSKYWGNGSTSSS
                     DGMRVQVGVSSLHADANPHYGTGKGTTIYRFTSDQFSSFYTKVINTNARDAVHVIDGL
                     LHHESELSIEEHYTDTAGYTDQVFGLSHILGFRFAPRMRDLSDSKLYVMDKSNVYSKI
                     DSILRGKINIKIIQDNYDDVLRLAHSIREGRVSASLILGKLGS"
                     /citation=[1]
                     /partial
BASE COUNT     1102 a    545 c    653 g    951 t
ORIGIN
        1 aacgtttaag ggaaaaactt tacctatcta tagtacacat gatttttggc gttttctaat
       61 ccctaaaccc ttcccgtatt catcttccct aaaattgact tataatccga attagggaac
      121 aattattctt ggggagggaa tttaatggga aaagtttttg gatatgctcg tgtttctact
      181 caagatcaaa ttttagatct tcaaattgat gttttagaaa aagcaggcgc agctgttatt
      241 tacaaagaga aaataactgg aacgagaaaa gaacgtccag aattagaaca actcctaaag
      301 gctataagta aaggtgattc tgtggtcgtt tataagttgg atcgtatctc aaggtctaca
      361 aaacatctaa ttgaattggt tgaaaccttt gaagaaaaag aggtcaattt tatttccata
      421 caggataata ttgatacatc aacagctatg ggacgttttt tcttccgaac aatggcgagt
      481 attgctgaac tagaacgaga tattatcgtc gagcgtacaa aatcaggatt acaatctgca
      541 agaatgagag gccgaaatgg ggggagacct tccaaagacc ccaagttggt ggaacgtgct
      601 ttaaaactac attcatctaa acaatatagc atcaaagaaa ttacggatat gacgggtgtt
      661 agtaaatcag tgttgtatcg agctttagaa aaaaattaaa ccttcgggtt ggattagggg
      721 gattttcctg tgtatctaag agcaagagaa ctacttacac ctgatcaaag aaaggatttt
      781 ttgttaatcc cgtctacttt aagtgaattg ggaattagcg tattgactat acccttacac
      841 aagcatgata ttgaggtaat aagacgtaga agaagagatc ataatcgatt gggattcgct
      901 attcaaattt gtctttttcg ttaccctggt tggtctcttt cagatatcaa gaatgttcct
      961 gataaggtga taaactatgt tgctaatcag ttacaagtag atgcaagtga atttaagcta
     1021 tattctgaac gagagcaaac gaaacatgaa catatggagg aaatacgaaa gtattatggt
     1081 ttttctaatt tttcagcaca gtcatatcgg attatttcac aggcattact cccccatgca
     1141 attgaaaatt caaatgcact gttcctaata aggatgactt tagaagaaat gcgaaagcga
     1201 aaaatcattc tgcctgctat gacaacgatt gaaagattag tatgggaaac aaggcgaagg
     1261 gcagaggaga aagtatataa ttcactctat aaaccgttat ctccatggca aaaacaacaa
     1321 ttggagaaat taattgatac cccttccgat aaatccaaaa ctaagcttgg atggctaagg
     1381 gaaataccag gtcagtcatc accagatgct tttttaaaag taattgagag attagaatat
     1441 gttcgtttat taaatctttc aacagagtcc gaaaatattc actcgaatag gctgcttcag
     1501 cttgctaggc taggcgcccg atatgaacct cattcctttc ggaggtttaa tgagaataaa
     1561 aggtacgcta ttcttgtagc tcacctttta actcttagtc aagatctaat cgatcaagct
     1621 atcgaaattc atgaccggca aattatgatc ttacagtcaa agggtagaaa agcacaagaa
     1681 gaactgcaaa agcaaaatgg aaaatctatc aatgaaaaag tactgcactt tgcggatatt
     1741 ggtgaagctt tagtaaaagc acgaaatgaa gagctagatc catttgaagt attagagaaa
     1801 ataatgcctt gggaaaggat tgttgattct atagaagaag caaaacgttt agctcatcct
     1861 atggactacg attacttaga tttgttggtc acccggttta gttaccttag aaagtataca
     1921 ccggtacttc taagtaaatt ggagttccgt acgactcagg cttctgagcc tttattgcgc
     1981 gctctaaatg tcttgcgtga aattaaccat aataagaaac gccatattcc cgaaggcgcg
     2041 ccattggatt ttgtgccaaa gagatggcag aaacatgtct atgatgaaga tggaaatatt
     2101 aatcgaaagt attatgaatt agcagcatta accgaattaa aaaaccatat ccgttcagga
     2161 gatatatggg ttgctggaag caggttacat aaggattgtg aagaatactt agtcactaaa
     2221 gataattggg atgaaactaa gaatacaggc aatagattac ctgttggtat ctcaactcaa
     2281 gaatatataa ttgagagaaa tgctgcttta aataaaagat tagattatat attggaaaac
     2341 atagattctc ttgagggaat aagtattgac aagtcaagga tgcgacttga ccgtttagag
     2401 aaagatacgc cggaagatgc caaatctctt agccaaactt tatataacat gttaccacgg
     2461 gtaaaactta ctgatttatt gattgaagtt tcaaattgga caggattcga tgaacacctt
     2521 gctcatgcgt ctagtaatcg tccacctaag ggagaagaaa agtctattgt aatggctacg
     2581 attatggcta tgggaaccaa tatcggtttt accaaaatgg ttgaagcaac accaggaatc
     2641 acctatcatc agttagctaa tgcagcccaa tggcgattac atgaggattc tttgagtaaa
     2701 gctcaagcta ctttagtaaa ttttcaacat ctttctttat caaaatattg gggaaatggg
     2761 agtacctcat cttcggatgg catgcgtgta caagttggcg tctcttctct tcatgctgat
     2821 gctaacccac actacggaac agggaaagga actactatct atagattcac cagcgatcaa
     2881 ttttcttctt tttatacaaa ggttattaac acaaacgcta gggatgctgt acatgtcatc
     2941 gatggattgc ttcatcatga atcagaatta tccattgaag agcattatac ggatacggct
     3001 ggttacaccg atcaggtttt tggattaagc catatattag gttttcgctt tgctcctaga
     3061 atgcgagatc tatctgactc taaactttat gtcatggata aatccaatgt ttattcaaag
     3121 attgacagca ttctacgagg aaaaatcaac attaagatta ttcaagataa ctatgatgat
     3181 gtcttacgat tagcacattc catacgggaa ggtagagtat cggcttcact tattttagga
     3241 aagttaggat c
//