From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: E.faecalis sprE gene for serine proteinase homologue
Date: 15 Aug 92 10:01:44 GMT
Distribution: bionet
Checksum: 30383     4

LOCUS       ENESPREG     1085 bp ds-DNA             BCT       15-AUG-1992
DEFINITION  E.faecalis sprE gene for serine proteinase homologue
ACCESSION   Z12296
KEYWORDS    serine proteinase homologue; sprE gene.
SOURCE      Enterococcus faecalis DNA.
  ORGANISM  Enterococcus faecalis
            Prokaryotae; Firmicutes; Firmibacteria; Gram-positive cocci;
            Streptococcaceae.
REFERENCE   1  (bases 1 to 1085)
  AUTHORS   Su,Y.A. and Clewell,D.B.
  TITLE     A gene (sprE) downstream of gelE of Enterococcus faecalis OG1-10
            resembles serine proteinase determinant of Staphylococcus aureus
            strain V8
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
REFERENCE   2  (bases 1 to 1085)
  AUTHORS   Clewell,D.B.
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
COMMENT     *source: strain=OG1-10; *source: is_macronuclear=N; *source:
            is_proviral=N; *source: is_germline=N;
            From EMBL    entry EFSPREG;  dated 06-AUG-1992.
FEATURES             Location/Qualifiers
     RBS             75..78
                     /note="Consensus"
     CDS             91..945
                     /product="Staphylococcal serineproteinase homologue"
                     /gene="sprE"
                     /codon_start=1
                     /translation="MKKFSIRKISAGFLFLILVTLIAGFSLSANAEEYIVPAESHSRQ
                     KRSLLDPEDRRQEVADTTEAPFASIGRIISPASKPGYISLGTGFVVGTNTIVTNNHVA
                     ESFKNAKVLNPNAKDDAWFYPGRDGSATPFGKFKVIDVAFSPNADIAVVTVGKQNDRP
                     DGPELGEILTPFVLKKFESSDTHVTISGYPGEKNHTQWSHENDLFTSNFTDLENPLLF
                     YDIDTTGGQSGSPIYNAQFEVVGVHSNGGIKQTGNHGQRLNEVNYNFIVNRVNEEENK
                     RLSAVPAA"
     terminator      954..1008
                     /note="factor-independent"
BASE COUNT      364 a    189 c    223 g    309 t
ORIGIN
        1 caggtaaacc aaccaagtga atctgttctg gtcaatgaat gaaaaaaatt ccccaattaa
       61 ataaaaatgg ataaggagtg gcatgatctt atgaaaaagt tctccatacg aaaaattagt
      121 gctggttttt tgtttctgat tttagtaact ttgatagccg gttttagcct gtctgcaaat
      181 gcagaagagt atatcgttcc tgccgaaagt cattcacgac aaaaaagatc gttactggac
      241 cctgaggaca gaagacaaga agtggcagat acaaccgaag cgccttttgc gtcaatcgga
      301 agaatcattt cccctgccag taaaccaggc tatatttctt taggaacagg ctttgttgtt
      361 ggaactaata caattgtcac caataatcat gtggctgaaa gttttaagaa tgccaaagta
      421 ttaaatccga atgccaaaga tgatgcttgg ttttatccag gtcgtgatgg cagtgcgaca
      481 ccatttggca aattcaaagt gattgatgta gctttttccc cgaatgcgga tattgcggta
      541 gtgactgtcg gcaaacaaaa cgatcgtcca gatggcccag agttgggaga aattttaacg
      601 ccatttgttt tgaaaaagtt tgaatcttca gatacccatg tcacaatatc aggctatcca
      661 ggtgagaaaa accacacgca atggtcgcat gaaaatgatt tgtttacatc taactttaca
      721 gacttagaaa atccattact attttatgat atcgatacaa caggtggtca atctggttcc
      781 ccaatctata atgctcagtt tgaagtagtt ggtgttcatt ccaatggcgg cattaagcaa
      841 acaggaaacc atggtcaaag actaaatgaa gtgaattata actttattgt caatcgagtg
      901 aatgaagaag aaaataaacg tttatccgct gtgccagcag cgtaaagaac cataaaaata
      961 ctcgaaattt cccaggatca cgggaaattt cgagtatttt tgttttttaa gatagtgtaa
     1021 gttacgatat aagtaccttg taattatcgg ttaaaagcat ataatagtgc tgaaaagaag
     1081 gcaaa
//