From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Pseudomonas aeruginosa anaerobic regulatory protein (anr) gene,
Date: 5 Jan 93 06:48:34 GMT
Distribution: bionet
Checksum: 57132 9

LOCUS       PSEANRPRO    3761 bp ds-DNA             BCT       04-JAN-1993
DEFINITION  Pseudomonas aeruginosa anaerobic regulatory protein (anr) gene,
            complete cds.
ACCESSION   M98276
KEYWORDS    anr gene; positive control element; regulatory protein.
SOURCE      Pseudomonas aeruginosa (strain PAO1) DNA.
  ORGANISM  Pseudomonas aeruginosa
            Prokaryotae; Gracilicutes; Scotobacteria; Pseudomonadaceae.
REFERENCE   1  (sites)
  AUTHORS   Zimmermann,A., Reimmann,C., Galimand,M. and Haas,D.
  TITLE     Anaerobic growth and cyanide synthesis of Pseudomonas aeruginosa
            depend on anr, a regulatory gene homologous with fnr of Escherichia
            coli
  JOURNAL   Mol. Microbiol. 5, 1483-1490 (1991)
  STANDARD  full automatic
REFERENCE   2  (sites)
  AUTHORS   Savioz,A., Zimmermann,A. and Haas,D.
  TITLE     RpoN-independent promoters having a conserved GG-N10-GC motif in
            Pseudomonas aeruginosa
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
REFERENCE   3  (bases 1 to 3761)
  AUTHORS   Zimmermann,A.
  TITLE     Anaerobe regulation des arginine-deaminase-operons von Pseudomonas
            aeruginosa durch das protein ANR
  JOURNAL   Thesis (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     CDS             22..504
                     /number=1
                     /citation=[3]
                     /note="putative;  ORF A"
                     /codon_start=1
                     /translation="MLDTIRWDADLIRRYDLSGPRYTSYPTAVQFHEGIGPFDQLHAL
                     RDSRKAGHPLSLYVHIPFCANICYYCACNKVITKDRGRSAPYLARLVREIEIVSRHLS
                     RAQVVEQLHFGGGTPTFLSPGQLRELMSQLRTHLNLLDDDSGDYGIEIDPREADWSTK
                     "
     CDS             564..1061
                     /number=2
                     /citation=[3]
                     /note="putative;  ORF B"
                     /codon_start=1
                     /translation="MPQRRINADDLPSPGQKLEMLQRTTEQLAAAGYRYIGMDHFALP
                     DDELASAQEDGTLQRNFQGYTTHGHCDLVGLGVSAISQIGDLYSQNSSDINDYQTSLD
                     NGQLAIRRGLHCNSDDRVRRAVIQQLICHFELAFEDIETEFGIDFRSYFAELWPDLEP
                     SPPTA"
     CDS             complement(1235..1702)
                     /number=3
                     /citation=[3]
                     /citation=[2]
                     /note="putative;  ORF X"
                     /codon_start=1
                     /translation="MLTLSGWITTQVPPSSRAAADAKAARTGTAEQAEDPAAGTDAAD
                     QHVQLSQAGRELAATMGVPVEEEDEDIPQELRPMVKMIRELKKKIEEKLRELQEAMRS
                     SDPGAKEARVPELQKELQQLNSALQTATAAMASAIKEMGISDPALIMKVMGSR"
     CDS             1948..2682
                     /number=4
                     /function="anaerobic regulatory protein analog to FNR of
                     E. coli"
                     /gene="anr"
                     /evidence="EXPERIMENTAL"
                     /citation=[1]
                     /citation=[3]
                     /citation=[2]
                     /product="ANR"
                     /codon_start=1
                     /translation="MAETIKVRALPQAHCKDCSLAPLCLPLSLTVEDMDSLDEIVKRG
                     RPLKKGEFLFRQGDPFGSVFAVRSGALKTFSITDAGEEQITGFHLPSELVGLSGMDTE
                     TYPVSAQALETTSVCEIPFERLDELSEQLPQLRRQLMRLMSREIRDDQQMMLLLSKKT
                     ADERIATFLVNLSARFRARGFSAQQFRLAMSRNEIGNYLGLAVETVSRVFTRFQQNGL
                     ISAEGKEVHILDSIELCALAGGQLEG"
     CDS             2768..3316
                     /number=5
                     /citation=[3]
                     /note="putative;  analog to the apt gene of E. coli; ORF
                     C"
                     /codon_start=1
                     /translation="MIFDEFTLKSQIRAVPDFPKPGVVFRDITPLFQSPRALRMTVDS
                     FVQRYIEADFSHIGAMDARGFLIGSAVAYALNKPLVLFRKQGKLPADVLAEGYQTEYG
                     EAFLEVHADSLCEGDSVLIFDDLIATGGTLLAAASLVRRLGARVFEAAAIIDLPELGG
                     STRLQDAGISTFSLTAFALDER"
     CDS             3556..>3561
                     /number=6
                     /citation=[3]
                     /note="putative;  ORF D"
                     /codon_start=1
                     /translation="MT"
BASE COUNT      669 a   1337 c   1060 g    695 t
ORIGIN      
        1 gaattcccgg ggatctctgg catgctcgac accattcgct gggacgctga cctgattcgg
       61 cgctacgacc tttccggccc gcgttacacc tcctatccga ccgccgtgca gttccacgaa
      121 ggcatcggcc cgttcgacca gttgcacgcc ctgcgcgaca gccggaaggc cggacacccc
      181 ctgtcgctct acgtgcacat cccgttctgc gccaacatct gctactactg cgcctgcaac
      241 aaggtgatca ccaaggatcg cgggcgcagc gcgccgtacc ttgcgcgcct ggtccgcgag
      301 atcgagatcg tcagccgcca cctctcccgc gcgcaggtgg tcgagcaact gcacttcggt
      361 ggcggaaccc cgaccttcct cagtcccggc cagttgcgcg aactcatgtc gcaattgcgc
      421 acccacctca acctgctgga cgacgattcg ggcgactacg gcatcgagat cgacccgcgc
      481 gaggccgact ggtcgacgaa gtgatcgcgc tacagccgga tcgcctctcg gtgttcaact
      541 acgcccacct gcccgagcgt ttcatgccgc agcggcggat caacgccgac gacctgccga
      601 gcccgggcca gaagctggaa atgctccagc gcaccaccga gcaactcgcc gcggcgggct
      661 accgctatat cggcatggat cacttcgccc tacccgacga cgagctggcc agcgcccagg
      721 aagacggcac cctgcaacgc aatttccagg gctacaccac gcacgggcac tgtgacctgg
      781 taggtctcgg ggtctcggcg atcagccaga tcggcgacct ctacagccag aacagcagcg
      841 acatcaacga ctaccagacc agcctcgaca acggccagtt ggcgatccgt cgcggcctgc
      901 actgcaacag cgacgaccgg gtgcggcgcg cggtgatcca gcaactgatc tgccacttcg
      961 aactggcctt cgaggatatc gagacagagt tcggcatcga cttccgcagt tacttcgccg
     1021 agctgtggcc cgatctcgaa ccttcgccgc cgacggcctg atccgcctgg acgccaaggg
     1081 catcgacatc acgtcttccg gacgtctgct ggtgcgttcg atctgcatgc tgttcgaccg
     1141 ctacctgccc agcctcaacc ggcagcgctt ctcccgggtg atctgaaagc gcaccgccct
     1201 gcccgcacga cagggcggtg aatccccgcc ccttctaccg gctgcccatg accttcatga
     1261 tcagcgccgg gtcgctgatg cccatctcct tgatggccga tgccatcgct gccgtcgccg
     1321 tctgcagggc ggagttgagt tgttgcagct ccttctgcaa ctcaggcacg cgagcctcct
     1381 tcgcccccgg atcggacgac ctcatggcct cctgcagctc cctgagcttc tcttcgatct
     1441 tcttcttcaa ctcgcggatc atcttcacca tcggccgcaa ctcctgcggg atatcctcgt
     1501 cctcctcctc caccggcacc cccatggtag cggccagctc gcgccctgcc tgggaaagct
     1561 gtacatgctg atcggcggcg tcggtgccgg cggccgggtc ttccgcctgc tcggcggtgc
     1621 cggtccgtgc ggccttggcg tccgcggcgg cgcgcgatga gggcggcacc tgggtggtga
     1681 tccagccact gagggtcaac attccagtca ctccgggaaa aatggaattc ttccattgga
     1741 tcggcccacg cgtcgcgaac ttgagccccc ttttcgtcgc cccttgacag ggtgcgacag
     1801 gtagtcgcag ttgtttgacg caagtcactg attggaaacg ccatcggcct gtcagaaatg
     1861 gtcgttgcca gacctatggc tggcacccgc atcgcggctg cgttaccctt actcctgttg
     1921 tgcctttaac ctagcaagga cccctcaatg gccgaaacca tcaaggtgcg cgcactgccc
     1981 caagcacact gcaaggattg cagtctggcc ccgctgtgcc tccccctgtc gctgaccgtg
     2041 gaagacatgg attcgctgga cgagatcgtc aagcgcggtc gtcccctgaa gaaaggcgaa
     2101 ttcctgttcc gccagggtga tcctttcggc tcggtctttg ccgtgcgctc cggcgccctg
     2161 aaaaccttca gcatcaccga tgccggcgaa gagcagatca ccggcttcca cctgcccagc
     2221 gagctggtcg gcctgtccgg gatggatacc gagacctatc cggtatccgc ccaggccctg
     2281 gaaaccacct cggtctgcga gattcccttc gagcgcctgg acgaactgtc cgagcagctg
     2341 ccgcagctgc gccgtcaact gatgcgtctg atgagccggg aaatccgcga tgaccagcag
     2401 atgatgctgc tgctgtcgaa gaagactgcc gacgagcgca tcgccacctt cctggtcaac
     2461 ctgtcggcgc gcttccgtgc ccgtggcttc tccgcccagc agttccgcct ggccatgtcg
     2521 cgcaacgaga tcggcaacta tctcggcctg gccgtggaaa ccgtgtcgcg ggtcttcacc
     2581 cgcttccagc agaacggcct gatcagcgcg gaaggcaagg aagtgcacat cctcgactcc
     2641 atcgagctgt gcgccctcgc cggcggccag ctggaaggct gaagcgcctg cgaaccgcca
     2701 acgggcccgc ctcagcgcgg gcccgttgta tgatgggccc cgaaaaacgc ccagcccagg
     2761 atcttccatg attttcgacg agttcaccct caagtcgcag atccgcgccg tgccggactt
     2821 tcccaagccc ggcgtggtgt tccgcgacat caccccgctg ttccagtcgc cacgcgcgct
     2881 gcgcatgacc gtggacagct tcgtccagcg ctacatcgag gccgatttca gccacatcgg
     2941 cgccatggat gcccgcggct tcctcatcgg ctcggcggtg gcctatgcgc tgaacaagcc
     3001 gctggtcctg ttccgcaagc agggcaagct acccgccgac gtactcgccg agggctacca
     3061 gaccgaatac ggcgaggcct tcctcgaagt acatgccgac agcctctgcg aaggcgacag
     3121 cgtactgatc ttcgacgacc tcatcgccac cggcgggacc ctgctggcgg ccgcgagcct
     3181 ggtacgacgc cttggggcca gggtcttcga ggccgcggcg atcatcgacc tgccggaact
     3241 cggcggctcc actcgcctgc aggacgccgg catctccacc ttcagtctca ccgcgttcgc
     3301 tctcgacgag cgttgatacg ccggggggcg ccctggcgcc ccgccctcgc tccttccccc
     3361 ccatcttccg tctggtcgag ccgacctggc cattaatgac ttcgaaatgg ccaggaatga
     3421 cctcccgctg ttggacaatc ctgcaataat tacatcagct aatgacacat tcagaggtgt
     3481 tatagcgaac acgccatagg gaacccgagg aaacctgtgc gtttcccctc cccagaacaa
     3541 caaaaggagt tagccatgac tgccactggc accgcctccc cggttcgcgc caatttcccc
     3601 gtgcggcgga tggatttcag cttcagcgaa accccgaagt actggtggga cggccaaccc
     3661 ttcatgaccc agttcatgaa caacctgtcc tcgctcttcc cctacggcga aaaattcttc
     3721 gtcgatagcg tacgcgcggt gcgcgaccgc ctggaggatc c
//