From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: T.thermophilus pheS and pheT genes encoding phenylalanyl-tRNA
Date: 24 Jul 92 10:10:42 GMT
Distribution: bionet
Checksum: 23995     9

LOCUS       TTHPHESTG    4171 bp ds-DNA             BCT       24-JUL-1992
DEFINITION  T.thermophilus pheS and pheT genes encoding phenylalanyl-tRNA
            synthetase alpha and beta subunits
ACCESSION   Z12118
KEYWORDS    phenylalanyl-tRNA synthetase;
            phenylalanyl-tRNA synthetase alpha subunit;
            phenylalanyl-tRNA synthetase beta subunit.
SOURCE      Thermus thermophilus DNA.
  ORGANISM  Thermus thermophilus
            Prokaryotae; Gracilicutes; Scotobacteria; Neisseriaceae.
REFERENCE   1  (bases 1 to 4171)
  AUTHORS   Keller,B.
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
REFERENCE   2  (bases 1 to 4171)
  AUTHORS   Keller,B., Kast,P. and Hennecke,H.
  TITLE     Cloning and sequence analysis of the phenylalanyl-tRNA synthetase
            genes (pheST) from Thermus thermophilus
  JOURNAL   FEBS Lett. 301, 83-88 (1992)
  STANDARD  full automatic
COMMENT     *source: strain=HB8; *source: is_macronuclear=N; *source:
            is_proviral=N; *source: is_germline=N;
            From EMBL    entry TTPHESTG;  dated 19-MAY-1992.
FEATURES             Location/Qualifiers
     CDS             327..1379
                     /product="Phenylalanyl tRNASynthetase, alpha subunit"
                     /gene="pheS"
                     /codon_start=1
                     /translation="MLEEALAAIQNARDLEELKALKARYLGKKGLLTQEMKGLSALPL
                     EERRKRGQELNAIKAALEAALEAREKALEEAALKEALERERVDVSLPGASLFSGGLHP
                     ITLMERELVEIFRALGYQAVEGPEVESEFFNFDALNIPEHHPARDMWDTFWLTGEGFR
                     LEGPLGEEVEGRLLLRTHTSPMQVRYMVAHTPPFRIVVPGRVFRFEQTDATHEAVFHQ
                     LEGLVVGEGIAMAHLKGAIYELAQALFGPDSKVRFQPVYFPFVEPGAQFAVWWPEGGK
                     WLELGGAGMVHPKVFQAVDAYRERLGLPPAYRGVTGFAFGLGVERLAMLRYGIPDIRY
                     FFGGRLKFLEQFKGVL"
     CDS             1376..3733
                     /product="Phenylalanyl tRNASynthetase, beta subunit"
                     /gene="pheT"
                     /codon_start=1
                     /translation="MRVPFSWLKAYVPELESPEVLEERLAGLGFETDRIERVFPIPRG
                     VVFARVLEAHPIPGTRLKRLVLDAGRTVEVVSGAENARKGIGVALALPGTELPGLGQK
                     VGERVIQGVRSFGMALSPRELGVGEYGGGLLEFPEDALPPGTPLSEAWPEEVVLDLEV
                     TPNRPDALGLLGLARDLHALGYALVEPEAALKAEALPLPFALKVEDPEGAPHFTLGYA
                     FGLRVAPSPLWMQRALFAAGMRPINNVVDVTNYVMLERAQPMHAFDLRFVGEGIAVRR
                     AREGERLKTLDGVERTLHPEDLVIAGWRGEESFPLGLAGVMGGAESEVREDTEAIALE
                     VACFDPVSIRKTARRHGLRTEASHRFERGVDPLGAGPRPEAGLKPPPGLAGARVAEAL
                     LEAGSPKPPEAIPFRPEYANRLLGTSYPEAEQIAILKRLGCRVEGEGPTYRVTPPSHR
                     LDLRLEEDLVEEVARIQGYETIPLALPAFFPAPDNRGVEAPYRKEQRLREVLSGLGFQ
                     EVYTYSFMDPEDARRFRLDPPRLLLLNPLAPEKAALRTHLFPGLVRVLKENLDLDRPE
                     RALLFEVGRVFREREETHLAGLLFGEGVGLPWAKERLSGYFLLKGYLEALFARLGLAF
                     RVEAQAFPFLHPGVSGRVLVEGEEVGFLGALHPEIAQELELPPVHLFELRLPLPDKPL
                     AFQDPSRHPAAFRDLAVVVPAPTPYGEVEALVREAAGPYLESLALFDLYQGPPLPEGH
                     KSLAFHLRFRHPKRTLRDEEVEEAVSRVAEALRARGFGLRGLDTP"
BASE COUNT      594 a   1530 c   1422 g    625 t
ORIGIN
        1 ggtacccccg ccggttaggg cgggccaccc cggcgaaggg cgaaaggagg tccaggctct
       61 cccggaccaa ggcggccagg tcctcgtccc cgggccagaa gaggcggtag gcctccgtgg
      121 acttgtcgct ggtgcaggaa aggggggcct cccgctccag cacccagggc ctgagccccc
      181 ttcgcgccaa aaagtaggcg gcggaaaggc cggcaaggcc cccgcccacc accaaagcct
      241 ccacgcgccg ctccatgccc aaagcctcct tccgggttcg cgcccatcgt acaccgaggc
      301 ggtatcctct tgggaagagg ggtgccatgc tggaagaagc cttggccgcc atccaaaacg
      361 cccgggacct ggaggagctc aaagccctca aggcccgcta cctggggaag aagggcctct
      421 tgacccagga gatgaagggc ctttcggccc ttcccctgga ggagcgccgg aaacggggcc
      481 aggagctgaa cgccatcaag gccgccctcg aggcggccct ggaggcgcgg gaaaaggccc
      541 tggaggaggc ggccctcaag gaggccttgg agcgggagcg ggtggacgtg agccttcccg
      601 gagcaagcct cttctccggg gggctccacc ccatcaccct catggagcgg gagctcgtgg
      661 agatcttccg cgccctgggc taccaggcgg tggagggccc cgaggtggag agcgagttct
      721 tcaactttga cgccctgaac atccccgaac accacccggc gcgggacatg tgggacacct
      781 tctggctcac tggggagggc tttaggctgg aaggccccct gggggaggag gtggaggggc
      841 gcctcctcct caggacccac acctccccca tgcaggtccg gtacatggtg gcccacaccc
      901 cccctttccg catcgtggtc ccgggccggg tcttccgctt tgagcagacg gacgccaccc
      961 acgaggccgt cttccaccag ctggagggcc tggtggtggg cgaggggatc gccatggccc
     1021 acctcaaggg ggcgatttac gagctcgccc aggccctctt cggccccgac tccaaggtgc
     1081 gcttccagcc cgtctacttc cccttcgtgg agcccggggc ccagttcgcc gtgtggtggc
     1141 ccgagggggg gaagtggctg gagctgggcg gggccgggat ggtccacccc aaggtcttcc
     1201 aggcggtgga cgcctaccgg gaaaggctcg gcctgccccc ggcctaccgg ggggtcacgg
     1261 gcttcgcctt cgggctcggg gtggagcgcc tcgccatgct ccgctacggc atccccgaca
     1321 tccgctactt cttcgggggc aggctcaagt tcctggagca gttcaagggg gttctatgag
     1381 ggtgcccttc tcctggctaa aagcctacgt gcccgagctg gaaagccccg aggtcctgga
     1441 ggagcgcctg gcgggcctgg ggtttgaaac ggaccggata gagcgggtct tccccatccc
     1501 aagaggggtg gtcttcgccc gggtcctgga ggcccacccc atccccggca cccggcttaa
     1561 gcgcctggtc ctggacgcgg gccggacggt ggaagtggtc tcgggggcgg aaaacgcccg
     1621 aaaaggaatc ggggtggccc tggccctccc cgggacggag cttcccggcc tgggccaaaa
     1681 ggtgggggaa cgggtcatcc aaggggtgcg gtccttcggc atggccctct ctccccggga
     1741 gctcggggta ggggagtacg gcggggggct tctggagttc cccgaggacg ccctcccccc
     1801 cggcaccccc ctttcggagg cctggccgga ggaggtggtg ctggacctcg aggtcacccc
     1861 gaaccgcccg gacgccctgg gccttttggg cctcgcccgg gacctccacg ccctgggcta
     1921 cgccctggtg gagcccgaag cggccctgaa ggcggaggcc cttcccctcc ccttcgccct
     1981 caaggtggag gacccggagg gcgcccccca cttcaccctg ggctacgcct tcggcctaag
     2041 ggtggcccca agccccctct ggatgcagcg ggccctcttc gccgcgggca tgcggcccat
     2101 caacaacgtc gtggacgtga ccaactacgt catgctggaa agggcccagc ccatgcacgc
     2161 ctttgacctg cgcttcgtag gagaggggat cgcggtgcgc cgggcgcggg aaggggagcg
     2221 gcttaagacc ctggacgggg tggaaagaac cctccacccc gaggacctgg tgatcgccgg
     2281 gtggcggggg gaggagagct tccccttggg cctcgccggg gtcatgggcg gggcggagag
     2341 cgaggtccgg gaggacacgg aggccatcgc cttggaggtg gcctgctttg acccggtctc
     2401 catccgcaag accgcccgcc gccacggcct gcgcaccgag gcgagccacc gctttgagcg
     2461 gggggtggac cccctgggcg caggtccccg cccagaggcg ggccttaagc ctcctccagg
     2521 cctggcgggg gcccgggtgg ccgaggccct cctcgaggcg ggaagcccca agcccccgga
     2581 ggccatcccc ttccgccccg agtacgccaa ccgcctcctg ggcacgtcct accccgaggc
     2641 cgagcagatc gccatcctca agcggctcgg gtgccgggtg gaaggcgaag gccccaccta
     2701 ccgggtcacc ccgcccagcc accgcctgga cctcaggctg gaggaggacc tggtggagga
     2761 ggtggcccgc atccagggct acgagaccat ccccttggcc ctccccgcct tcttccccgc
     2821 ccccgacaac cgcggggtag aagcccccta ccggaaggag cagcgcctaa gggaagtcct
     2881 ctccgggctc ggcttccagg aggtctacac ctacagcttc atggaccccg aggacgcccg
     2941 ccgcttccgc cttgatcccc cccgcctcct cctcctcaac cccctggccc cggaaaaggc
     3001 ggccctcagg acccacctct tccccggcct ggtccgggtg ctgaaggaga acctggacct
     3061 ggaccggccc gagcgggccc tcctctttga ggtgggccgg gtcttccggg agcgggagga
     3121 gacccacctg gccggcctcc tcttcggcga gggggtgggc ctcccctggg ccaaggagcg
     3181 gctttccggc tacttcctcc tcaaggggta cctcgaggcc ctcttcgccc ggctcggcct
     3241 ggccttccgg gtggaggccc aggccttccc cttcctccac cccggggtct cgggacgggt
     3301 cctggtggag ggggaggagg tgggcttcct cggcgccctc caccccgaga tcgcccagga
     3361 gctggagctt ccccccgtcc acctctttga gctccgcctg cccctcccgg acaaacccct
     3421 cgccttccaa gacccctccc gccaccccgc cgccttccgc gacctggccg tggtggtccc
     3481 cgcccccacc ccctacggcg aggtggaggc tttggtgcgg gaggccgcag gcccctacct
     3541 ggaaagcctc gccctctttg acctctacca aggcccgccc ctgcccgaag gccacaagag
     3601 cctcgccttc cacctccgct tccgccaccc caagcgcacc ctgcgggacg aggaggtgga
     3661 ggaggccgtc tcccgggtgg cggaggccct cagggccagg gggttcggcc tgcgcggcct
     3721 ggacaccccg taatgccacc ccatgctggc ttgcgccagc atgggggccc cggcaaaagg
     3781 ccctcagagg gcttttccgg attagccggg cgaaggaacc ggggagaaaa ggtataagct
     3841 gggggcatgc tcacctgggt ggacctcctg gccctcatgg ttctggccct ctccctggcc
     3901 ctggggtacc ggggggggct tgtcctcgcc tgggtcgggc tcctgggcct tcccctctac
     3961 gcggccgccc tggccctcgg cctccccgcc ttctggaccg ccctcgccct ggggctcgtc
     4021 ctgggggcct tggccaaaag cctccccctc ttcctctccg aggcggcgga aaggggcctc
     4081 gggcttctcg ggggcggcct cctcggcctc ttcctcgcgg cggccatctg gacgggcttc
     4141 cccagcgagc ccgccccttc cggggggatc c
//