From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: S.kluyveri HIS3 and PET56 genes for imidazoleglycerol phosphate
Date: 4 Sep 92 10:07:38 GMT
Distribution: bionet
Checksum: 48650     7

LOCUS       YSCHIS3G     2569 bp ds-DNA             PLN       04-SEP-1992
DEFINITION  S.kluyveri HIS3 and PET56 genes for imidazoleglycerol phosphate
            dehydratase and PET56 protein
ACCESSION   Z14125
KEYWORDS    imidazoleglycerolphosphate dehydratase; k-PET56 protein.
SOURCE      Saccharomyces kluyveri DNA.
  ORGANISM  Saccharomyces kluyveri
            Eukaryota; Fungi; Eumycota; Hemiascomycetes; Endomycetales;
            Saccharomycetaceae.
REFERENCE   1  (bases 1 to 2569)
  AUTHORS   Weinstock,K.G. and Strathern,J.N.
  TITLE     Molecular genetics in Saccharomyces kluyveri: the HIS3 homolog and
            its use as a selectable marker gene in S. kluyveri and S.
            cerevisiae
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
REFERENCE   2  (bases 1 to 2569)
  AUTHORS   Weinstock,K.G.
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
COMMENT     *source: strain=CBS3082; *source: clone=PKW301; *source:
            chromosome=Chromosome band VIII; *source: is_macronuclear=N;
            *source: is_proviral=N; *source: is_germline=N;
            From EMBL    entry SKHIS3G;  dated 29-JUL-1992.
FEATURES             Location/Qualifiers
     CDS             complement(<1..416)
                     /product="k-PET56 protein"
                     /gene="k-PET56"
                     /codon_start=1
                     /translation="MNNQPCSIVWRRFLTSKVKPAVRIPNENLKANKPTNFTKNFPLN
                     ERTKAWERAGEDKESWFKRKYAHVHAKQKSQPQVDLYGKKEAHYSRLKQDILSSKRQQ
                     EEHKSKYSRKSVTLGLRPNPLMEYIYGTNSVIAALNS"
     CDS             706..1404
                     /EC_number="4.2.1.19"
                     /product="imidazoleglycerolphosphate dehydratase"
                     /gene="k-HIS3"
                     /codon_start=1
                     /translation="MTEPAQKKQKQTVQERKAFISRITNETKIQIAISLNGGYIQIKD
                     SILPAKKDDDVASQATQSQVIDIHTGVGFLDHMIHALAKHSGWSLIVECIGDLHIDDH
                     HTTEDCGIALGQAFKEAMGAVRGVKRFGTGFAPLDEALSRAVVDLSNRPFAVIDLGLK
                     REMIGDLSTEMIPHFLESFAEAARITLHVDCLRGFNDHHRSESAFKALAVAIREAISS
                     NGTNDVPSTKGVLM"
     CDS             complement(1543..>2569)
                     /note="k-ORF1 is a large open reading frame ofunknown
                     function"
                     /product="k-ORF1 protein"
                     /gene="k-ORF1"
                     /codon_start=2
                     /translation="NSRIAHVPKSKKPLNSASPRFHPTHQIIETKPSTLHRQEWGLKS
                     SIPSKVQTRYIIFNDLDTLERLTTFEPNAGSQWSRLRFQELGVAPSYNAGKSNPLFEG
                     SSSSSNQLVPLSSLLNIDPTSSRTDVEKKLSQVKSLRKVFKKWLLSKDPEALKNKSFS
                     AKDMSDNAVEFLSESVGTNTGLNSASLNKVIGTGGLSYNIGGRLRQSPNGVVSKTVVP
                     GRFLNVEGNDRLAAIGGFVANAGSSSPNTSQIDYNMGDFIRELKFPFAVNRAFVQDNG
                     KVVLRANVISGMSSKARMQMSGKNYQQRPSRTTSPAVNPEDSTKYAEELLNILTNFDS
                     GNGKKLR"
BASE COUNT      731 a    512 c    515 g    811 t
ORIGIN
        1 gaattcaacg cagcaatcac actgtttgta ccgtatatat actccatcaa tggattgggt
       61 cttaaaccca aagtgacact tttcctggaa tatttagatt tatgctcttc ctgttgtctc
      121 ttggaactta gtatgtcctg tttaagtcta ctgtaatgtg cctctttctt gccatataaa
      181 tcgacttgtg gctgagactt ttgtttagca tggacatgcg catatttacg tttaaaccag
      241 gactccttat cttcaccagc acgttcccat gccttagttc tttcattcaa tgggaaattt
      301 ttggtaaaat ttgttggttt attagctttg agattctcat ttggaatacg gactgctggc
      361 tttaccttag atgtcagaaa tcttctccat acaattgaac aaggctggtt attcatttaa
      421 tacacggcgc tgcgctggtt actacttcac agagagacta cttttacaac gacagagaga
      481 tcctcaccaa atctgtctgt tattttctga cgaaaagaaa attttcttat aatccaaact
      541 ggagtatcat gtgacctaat cacgattaac ggcttaaaag aaatgtcgag aagaaaagaa
      601 gcttgctttt tctttttttt tttctttcaa aatgagtctt ctgagactct cgaaacgtag
      661 atagctaaaa caggttacta gacttatcat acacaggcca tagaaatgac agaaccagcc
      721 caaaaaaagc aaaaacaaac tgttcaggag cgcaaggcgt ttatctcccg tatcactaat
      781 gaaactaaaa ttcaaatcgc tatttcgctg aatggtggtt atattcaaat aaaagattcg
      841 attcttcctg caaagaagga tgacgatgta gcttcccaag ctactcagtc acaggtcatc
      901 gatattcaca caggtgttgg ctttttggat catatgatcc atgcgttggc aaaacactct
      961 ggttggtctc ttattgttga atgtatcggt gacctgcaca ttgacgatca ccatactacc
     1021 gaagattgcg gtatcgcatt agggcaagcg ttcaaagaag caatgggtgc tgtccgtggt
     1081 gtaaaaagat tcggtactgg gttcgcacca ttggatgagg cgctatcacg tgccgtagtc
     1141 gatttatcta atagaccatt tgctgtaatc gaccttggat tgaagagaga gatgattggt
     1201 gatttatcca ctgaaatgat tccacacttt ttggaaagtt tcgcggaggc ggccagaatt
     1261 actttgcatg ttgattgtct gagaggtttc aacgatcacc acagaagtga gagtgcgttc
     1321 aaggctttgg ctgttgccat aagagaagct atttctagca atggcaccaa tgacgttccc
     1381 tcaaccaaag gtgttttgat gtgatacaag gtgaaaaata atcatatcgt atttattcat
     1441 agaattgata ttcttatttt gaaaagtctt tatatacacg ttttttccgt tttttcgttt
     1501 tattcttaat atatacagtt ctcgagtcga ttcccatcct gcttatctca acttcttgcc
     1561 attaccgcta tcaaagtttg tcaaaatgtt caatagttct tcggcatatt tggttgaatc
     1621 ttcagggttg acggcaggag aagttgttct gcttggtctt tgctgatagt ttttgccaga
     1681 catctgcatt ctagctttgg agctcatacc actaattaca ttagccctta aaacaacttt
     1741 accattatct tgcacaaaag cacggttaac ggcaaatggg aatttcagct ctctgataaa
     1801 gtcacccatg ttgtaatcaa tctgggatgt gttaggagat gaagagcctg cgttggcgac
     1861 aaaaccacca atggcagcta acctatcgtt gccttcaaca ttcaaaaatc tacctggaac
     1921 aacggtctta gacacaacac cgttgggaga ttgtctcaat cttccaccga tgttgtaaga
     1981 tagaccacca gtaccaatca ctttattcag agaggccgag tttaggccag tattggtacc
     2041 aacagactcg gataaaaact cgacagcatt gtcactcata tctttagcac taaaactctt
     2101 atttttcaag gcctctggat ctttactcaa aagccatttc ttgaatacct tacgcaggct
     2161 tttaacttga gacaattttt tttccacgtc tgttctagat gaggtaggat caatattaag
     2221 caaagagctc aaaggaacta gctggtttga tgatgaagaa gaaccctcga acagtggatt
     2281 agatttaccc gcgttgtaac ttggtgcaac gcccaattcc tgaaacctca atctactcca
     2341 ctgggagccg gcattaggtt caaatgtggt tagacgttct aaggtatcca aatcattgaa
     2401 aatgatgtaa cgtgtttgca cctttgatgg aatagaagat tttaaacccc attcttgacg
     2461 atgtagtgtg gatggtttag tttcaattat ctgatgagtt gggtgaaatc ttggagatgc
     2521 agagttcaag ggcttcttag atttcggaac atgagcgata cgtgaattc
//