From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: D.pseudoobscura Adh gene for alcohol dehydrogenase
Date: 28 Dec 92 21:27:40 GMT
Distribution: bionet
Checksum: 49623 8

LOCUS       DROADHGN60   3471 bp ds-DNA             INV       01-DEC-1992
DEFINITION  D.pseudoobscura Adh gene for alcohol dehydrogenase
ACCESSION   X64469
KEYWORDS    ADH gene; alcohol dehydrogenase.
SOURCE      Drosophila pseudoobscura DNA.
  ORGANISM  Drosophila pseudoobscura
            Eukaryota; Animalia; Metazoa; Arthropoda; Uniramia; Insecta;
            Pterygota; Neoptera; Holometabola; Diptera; Brachycera;
            Cyclorrhapha; Schizophora; Drosophiloidea; Drosophilidae.
REFERENCE   1  (bases 1 to 3471)
  AUTHORS   Schaeffer,S.W. and Miller,E.L.
  TITLE     Estimates of gene flow in Drosophila pseudoobscura determined from
            nucleotide sequence analysis of the alcohol dehydrogenase region
  JOURNAL   Genetics 132, 471-480 (1992)
  STANDARD  full automatic
COMMENT     *source: strain=PS164; *source: isolate=Stemwinder Provincial Park,
            B.C. Canada; *source: chromosome=IV section 88;
               From EMBL    entry DPADHGN60;  dated 16-OCT-1992.
FEATURES             Location/Qualifiers
     mRNA            join(40..119,914..1019,1083..1487,1548..1963)
                     /note="adult mRNA (Adh)"
     mRNA            join(881..1019,1083..1487,1548..1963)
                     /note="larval mRNA (Adh)"
     promoter        8..14
                     /note="adult promoter (Adh)"
     prim_transcript 40..1963
                     /note="adult primary transcript (Adh)"
     exon            40..119
                     /note="adult exon 1a"
     intron          120..913
                     /note="adult intron"
     prim_transcript 881..1963
                     /note="larval primary transcript (Adh)"
     exon            881..1019
                     /note="larval exon 1"
     exon            914..1019
                     /note="adult exon 1b"
     intron          1020..1082
                     /number=1
     exon            1083..1487
                     /number=2
     intron          1488..1547
                     /number=2
     exon            1548..1963
                     /number=3
     polyA_signal    1940..1945
     polyA_site      1963
     CDS             join(927..1019,1083..1487,1548..1814)
                     /EC_number="1.1.1.1"
                     /gene="Adh"
                     /product="alcoholdehydrogenase"
                     /codon_start=1
                     /translation="MSLTNKNVVFVAGLGGIGLDTSRELVKRNLKNLVILDRIDNPAA
                     IAELKAINPKVTITFYPYDVTVPVAETTKLLKTIFAQVKTIDVLINGAGILDDHQIER
                     TIAVNYTGLVNTTTAILDFWDKRKGGPGGIICNIGSVTGFNAIYQVPVYSGSKAAVVN
                     FTSSLAKLAPITGVTAYTVNPGITKTTLVHKFNSWLDVEPRVAEKLLEHPTQTSQQCA
                     ENFVKAIELNKNGAIWKLDLGTLEPITWTQHWDSGI"
BASE COUNT      973 a    790 c    798 g    910 t
ORIGIN      
        1 cgtagtgtat ttaaggcact gcacatgtcg aggaccacga ttattgtcta cagtgcagtc
       61 agtagttgtc aggtgcagca ggcggaccag catttcttta aatctactca atcgatcaag
      121 taagtaactg taatccaagt acagtcggcc catcccactc ggttctgccg taactcaaga
      181 aatttcttga tagcgcagtt ccagttgtaa aacgtcgttt aaaaaaacat ctcgttggag
      241 cttgggttgg aaaacgttcg agttgggcgt agaaaagttc aaatataaaa tatcaaaaaa
      301 gagaaaatat cttcccaaag cagctgtcca ttttttgtta aagtgataga tcaaaaattc
      361 gtttgacgta tttcccaagt gctctttatg caaataattt cctcaattct aattctacaa
      421 gaagcacttt tggctcttgt ccagaaaaaa acaatttgtt tacacttttc ggttgataac
      481 agaagcggac cgacggctaa gtaacataac agtcgcctgt taagtaatgc cctcactctc
      541 tgttagatgg ccgcgcgtgt cgactccgat agaccagcag acacgcagta gttttcctca
      601 agaacctgaa ctctaaacat agacataatt tacgatacct tgagactcaa ataaaaatac
      661 aagtatgagt ggtattctat tcctgattag actcgcacac acacgtacga gtattaacat
      721 aaatttgccg gcaaataacc aaaacaaaac gaacgcaaac gatccgaaca cgtaatgcga
      781 gagataagaa acagaaaagc tcgacgtgaa agaagcttct ggcggaagct cttgccgaac
      841 tccctataaa tacgagacag aaaaccagaa taaatataat aacaaactgt tgaattgtcc
      901 catcgattcc cagatcaaaa gacagaatgt ctctcaccaa caagaacgtc gttttcgtgg
      961 ccggtctggg cggcattggc ctggacacca gccgggagtt ggtcaagcgt aatctgaagg
     1021 taagagagaa cgaattccat ggaatctatg gaatcctaaa tttaaaaatt catttatttt
     1081 agaacctggt catcctggat cgcattgaca atccggctgc cattgccgag ctgaaggcaa
     1141 tcaatcccaa ggtgaccatc accttctatc cctacgatgt gactgtgccc gtcgctgaga
     1201 ccaccaagct cctgaagacc atctttgccc aggtgaagac catcgatgtc ctgatcaacg
     1261 gtgctggcat cctggacgat catcagatcg agcgcaccat tgccgttaac tacacgggcc
     1321 tggtcaacac caccacagcc attctggact tctgggacaa gcgcaagggc ggcccaggcg
     1381 gcatcatttg caacattggc tccgtcaccg gtttcaatgc catctaccag gtgcccgttt
     1441 actccggctc caaggcggcg gtggttaact ttacctcctc cctggcggta agaaatccta
     1501 caactatcct actccccaaa actaaatatt tctttgaaat cccatagaaa ctggctccca
     1561 ttactggtgt cactgcttac actgtcaatc ctggcatcac taagaccact ctggtccaca
     1621 aattcaactc gtggctggat gtggagcccc gtgtggcgga gaagctgctc gagcatccca
     1681 cccagacctc tcagcagtgc gccgagaact ttgtgaaggc cattgagctg aacaagaacg
     1741 gtgccatctg gaagttggat ttgggcacct tggagcccat cacatggacc cagcactggg
     1801 actcgggaat ctaaaccatc ccagagactc tatgggacat ggcgttttag cttttagttt
     1861 ctttttttcc actcaattgt tacgtatata catacacata tggcaataag gctgatttga
     1921 accccgtttt gaatatgata atacaaaatt atatttgaga aatttcaaca aaatcgataa
     1981 gagctatagc tctcttttaa ttaaaaaaaa ctagataaaa agagcaatgg tcagtggtgg
     2041 tcagtggtcg ttgtggcctc tatcgatttc cacacaaaaa ctttactgtt tagtaataga
     2101 aaagaacttg agaaaggcag ccaaaagaat gtacgatctg acgggcaagc atgtctgcta
     2161 tgtggctgac tgcggtggca ttgcactgga gaccagcaag gttctcatga ccaagaatat
     2221 agcggtgagt ggtagagagg tggtgtagag agtggagagt gttccgaagt ggttctggtt
     2281 cttaaacaag tctctagtct cttggctgct gctggtagct gtcgaaacta attaagttaa
     2341 tgactttttc atgttttgtt tttgtccgct gacttttgct gttcaaagct acaattaagt
     2401 ttatgttttg agtttgatct acttttgctt aagggaagta ctttcgatta cggattgctg
     2461 gcatataaaa atagatacca aggaatgtac atcatatcat attgattcta tcttgattct
     2521 ttgcccagaa actggccatc cttcagagcg tggaaaaccc cccggccatc gctcagttac
     2581 aatcgattaa gcacagcaca cagatcttct tctggacctt cgatgtgacc atggcccgcg
     2641 aggagatgaa gaagtacttc gatgaggtca tggtccagat ggactacatc gatgtgctga
     2701 tcaatggggc caccctgtgc gatgagcgga acattgatgc caccatcaat acgaatctga
     2761 ccggcatgat gaacaccgtg gccactgtgc tgccctatat ggaccgtaag atgggcggtt
     2821 cgggtggact gattgtgaat gtcacctccg tcataggact ggatccatcg ccggtctttt
     2881 gtgcgtacag tgcctcaaag tttggtgtga ttgggttcac ccgaagtcta gcggtgagtg
     2941 ggcgagatct taacttgaaa atgctctcta atgtcttcca ttctttcgca ggatcccttg
     3001 tattacaccc aaaatggtgt ggctgtcatg gccgtctgct gtggtcccac caaagtgttt
     3061 gtcgatcggg aactgactgc cttcctggaa tacggccaaa cctttgccga tcgcctgcgt
     3121 cgtgccccct gccaatcgac ggccgtctgc ggccagaata tagtaaatgc catcgagaga
     3181 tcggagaacg gacagatctg gattgccgac aagggcggcc tggagtcggt ggccctccac
     3241 tggtattggc atatggccga tcagtttgtg aactatatgc agagcaccga cgatgaggat
     3301 caggagtttt ttttggggca gcgatgaggc atgccttgat ccacggaata aaacatcaaa
     3361 tgtgtcacaa aaagtaggca acttgtttgt gggtctggaa atagatttga aatattttga
     3421 ggtcatatga ataatgacat aaatgagggt gcctgaaagt atgccaagtt t
//