From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Erwinia chrysanthemi secretory component (outC-outM) genes,
Date: 21 Dec 92 20:41:22 GMT
Distribution: bionet
Checksum: 34052 26

LOCUS       ERWOUTCM    12682 bp ds-DNA             BCT       20-NOV-1992
DEFINITION  Erwinia chrysanthemi secretory component (outC-outM) genes,
            complete cds.
ACCESSION   L02214
KEYWORDS    outC gene; outD gene; outE gene; outF gene; outG gene; outH gene;
            outI gene; outJ gene; outK gene; outL gene; outM gene;
            secretory component.
SOURCE      Erwinia chrysanthemi (strain AC4150) DNA.
  ORGANISM  Erwinia chrysanthemi
            Prokaryotae; Gram-negative facultatively anaerobic rods;
            Enterobacteriaceae.
REFERENCE   1  (bases 1 to 12682)
  AUTHORS   He,S.Y., Lindeberg,M., Chatterjee,A.K. and Collmer,A.
  TITLE     Cloned Erwinia chrysanthemi out genes enable Escherichia coli to
            selectively secrete a diverse family of heterologous proteins to
            its milieu
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 88, 1079-1083 (1991)
  STANDARD  full automatic
REFERENCE   2  (bases 1 to 12682)
  AUTHORS   Lindeberg,M. and Collmer,A.
  TITLE     Analysis of eight out genes in a cluster required for pectic enzyme
            secretion by Erwinia chrysanthemi: Protein sequence comparison with
            secretion genes from other Gram-negative bacteria
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     CDS             730..1548
                     /gene="outC"
                     /evidence="EXPERIMENTAL"
                     /product="secretory component"
                     /codon_start=1
                     /translation="MNISKLPPLSPSVIRRILFYLLMLLFCQQLAMIFWRVGLPDNSP
                     VASVQITPAQARQQPVTLNDFTLFGVSPEKNRSGALDASQMSNLPPSTLNLSLTGVMV
                     GDDTARSIAIISKDNEQFSRGVNEEVPGYNAKIVSIRPDKVVLQYQGRYEVLGLYNQE
                     ENSADGVSGAQLNEQLQQRASTTMSDYVSFSPVMNDNKLHGYRLNPGPKSDSFYRVGL
                     QDNDMAVALNGLDLRDEEQAKKAMERMADVHNFTLTVERDGQRQDIYMEFGGDE"
     CDS             1550..3688
                     /gene="outD"
                     /note="putative"
                     /citation=[1]
                     /citation=[2]
                     /product="secretory component"
                     /codon_start=1
                     /translation="MLGKGIKKSWGWLGLTVLLLGSPCGWAAEFSASFKGTDIQEFIN
                     TVSKNLNKTVIIDPTVRGTISVRSYDMMDEGQYYQFFLSVLDVYGFSVVPMDNGVLKV
                     IRSKDAKSSSIPLANNEQPGVGDELVTRVVPLNNVAARDLAPLLRQLNDNAGAGTVVH
                     YEPSNVLLMTGRAAVIKRLVDIVNTVDKTGDREMITVSLNYASAEDVAKLVNDLNKTD
                     EKNALPSTMLANVVADGRTNSVVVSGEENTSPCAVEMIRQLDRKQVAQGGTKVIYLKY
                     AKALDLIEVLAGNGTSGNRNSSSTNSSRPSSTRSSSTLNNSNSSSSGSSSGSGSSSSS
                     SSSSMGFGSAFGSANSSGGRTIVIQGKEVTVRAHDQTNSLIITRPPDIMRDLEQVINQ
                     LDIRRPQVLVEAIIAEIQDADGLNLGIQWANKRAGMTQFTNTGIPISTAMIGTDQFRS
                     DGTLTTAYASALSNFNGITAGFYRGNWSMLLTALSSDGKNDVLATPSIVTLDNMEATF
                     NVGQEVPVLTGSQTTVGSGDNIFNTVERKTVGIKLRVKPQINEGDSVLLQIEQEVSSV
                     AEGNGSSNSSLGVTFNTRTVNNAVMVTNRETVVVGGLLDKTAIETNNKVPLLGDIPWL
                     GSLFRSKTQTMSKRNLMLFLRPTIIRDPQQYQQASISKYNSFNNEQQQQRGQGNSVLD
                     NNTLRLSGGNTYTFRQVQSSISAFYQPEGR"
     CDS             3685..5181
                     /gene="outE"
                     /note="putative"
                     /citation=[2]
                     /product="secretory component"
                     /codon_start=1
                     /translation="MSDQPVHTSELRPVLPFAFARAQQILLLQDESASAAEVVCVPET
                     PALALLEVRRVAGVALTVSQVSPEEFERQLVMRYQRDSEEARRLMEDIGNDIDFYTLA
                     EELPDSDDLLDGEDDAPIIRLINAMLTEAIKHKASDIHIETFERHLLIRFRIDGVLRE
                     ILRPQRQLASLLVSRIKVMAKLDIAEKRVPQDGRMALRIGGRAIDVRVSTLPSNYGER
                     VVLRLLDKNSVRLDLETLGMAEHHRRQLDTLIHRPHGIILVTGPTASGKSTTLYAALS
                     PLNSAERNIMTVEDPIEYELEGIGQTQVNPKVDMTFARGLRAILRQDPDVVLVGEIRD
                     GETAQIAVQASLTGHLVLSTLHTNSALGALSRLQDMGIEPFLLSTSLLGVLAQRLVRT
                     LCPSCRQPYTIDHEQAEQTGLAAGTTLYHPGGCEKCNYSGYRGRTGIHELLLIDDTVR
                     AAIHRGESELGIARMLGAKRVTIRQDGLDKVLAGITTWEEVVRVTKEE"
     CDS             5183..6397
                     /gene="outF"
                     /note="putative"
                     /citation=[2]
                     /product="secretory component"
                     /codon_start=1
                     /translation="MALFQYQALNAQGKKSQGMQEADSARHARQLLREKGLVPVKIEE
                     QRGEAAPRSGFSLSFGRSHRIASDLALLTRQLATLVAALPLEEALDAVAKQSEKPKLS
                     ALMAAVRAKVVEGHSLAEAMGNFPGSFERLYCAMVAAGEASGHLDAVLNRLADYTEQR
                     HEMRSRIQQAMIYPCVLTLVAISVVSILLSAVVPKVVEQFIHMKQALPLSTRLLMSAS
                     DAVRTYGPWVVLLLVLAIMGFRVLLRQEKHRLVFHRRLLFLPVVGRVARGLNTARYAR
                     TLSILNSSAVPLLQAMRISGDVLTNDYARFRLGQATDAVREGVTLHKALEQTALFPPM
                     MRHMIASERRRARRHVNPRGDNQDREFSAQMTLVLGLFEPLLVVSMAGIVLFIVLAIL
                     QPILQLNTLMSM"
     CDS             6431..6892
                     /gene="outG"
                     /note="putative"
                     /citation=[2]
                     /product="secretory component"
                     /codon_start=1
                     /translation="MERRQRGFTLLEIMVVIVILGVLASLVVPNLMGNKEKADKQKAV
                     SDIVALESQLDMYKLDNSRYPTTEQGLGALVKKPTTPPEPRNYPQDGYIRRLPQDPWG
                     AEYQLVSPGRHGKIDVFSYGPDGMPDTDDDIGNWNVGNGAHNNGGNGNGNP"
     CDS             6895..7440
                     /gene="outH"
                     /note="putative"
                     /citation=[1]
                     /citation=[2]
                     /product="secretory component"
                     /codon_start=1
                     /translation="MRQRGFTLLEIMLVVLLAGVAATLVMMAIPAPKQQDSGWQIARF
                     KAQLQYAVEDSQMNDHILGIYIQPHRWQYALLQRQVVENSPEEQQRLRYVWIPWQPYR
                     MSVPSELPDSFHIELSTQVGAAGDGTGFSPGNGDPHVLILPGGEVTPFRLTLRNGNDS
                     AWLQVDTNGQVHTSPEAEQKG"
     CDS             7437..7814
                     /gene="outI"
                     /note="putative"
                     /citation=[1]
                     /citation=[2]
                     /product="secretory component"
                     /codon_start=1
                     /translation="MKQQGMTLLEVMVALVIFALAGLTVLKTTAQQANGLGRLEEKTF
                     ALWIAENQQAAMRLEKQWPQSQWVDGEVNFAGSLWFWRIQGIATADTRVRAIDVEVRH
                     DRDSRAADAVLRSYLVQPGEPAQ"
     CDS             7811..8431
                     /gene="outJ"
                     /note="putative"
                     /citation=[1]
                     /citation=[2]
                     /product="secretory component"
                     /codon_start=1
                     /translation="MKQPERGFTLLEVMLALAIFAALSLAASQVMNGVMRNDEASARK
                     EARLAELQRGFSLMERDFSQIVPRRSQGYELGFYAERYQLSSADWAVSFIRNGWLNPL
                     GIMPRSELQRVGYRLRGDTLERLFYDSSEPLSTQEPAVRPVLTGVTGFVLRFFGKDGW
                     QERWDDPANLPQGIAIVVTLRDYGEITRIFLITPRYGAATQRRSER"
     CDS             8546..9610
                     /gene="outK"
                     /note="putative"
                     /citation=[1]
                     /citation=[2]
                     /product="secretory component"
                     /codon_start=1
                     /translation="MSRRQRGVALLIVMLMLSLMVTIAASITERSGKAWQRTSNLLNR
                     TQARWYALGAEALISNVLQRDAQASPESTFIGQPWSKVDHQMMADGTEIRAQALDGQA
                     CLNLNALSPARNVTPNNASGNNTSGNNNAANGSSGNGNSPQPPKVGTSEQVPYAAQVF
                     RQLMIVLGEDPKQAERITDALRDWLDEDSEPLMNGAEDDSYVNFHPGNQRMTDVTELR
                     AVMGMDAALYRRLLPYVCVLPVDKLAINVNTLMPGSAPLLSALFMGDISLDMAERILQ
                     QRPPQGWRNLNDFMGMSALPESGKNGARQVLVIKSDWFFADIQIRVDDSEFYQRSLFH
                     RGKQIEVVQRQYGGYRTVNP"
     CDS             9607..10806
                     /gene="outL"
                     /note="putative"
                     /citation=[2]
                     /product="secretory component"
                     /codon_start=1
                     /translation="MSKAENTSGKQQLILRLSADTSDSLEWLIWSVSRHQTLTTGSGT
                     LESLQAVLADYPVISARVLVPSTDVTFHTLSLPRQSRRQLLQAIPFMLEEQVASDIDQ
                     LHFAVMDMHGDNATVAVVQKSRLRAWLNQCETLGVPVETVVPDVMALPRADSAWSAIS
                     HRNLWLFRLDSGIGMAAEENWYQSLLAFQPLPAVHCYSPVPASALTWQPQPVTDLLTL
                     AAQVNLSMSMDLRQGEYAPVKPWKQALLPWRNVLIALSAWLLLVLGESVWTHYQWYRQ
                     ADYWRQESVRVYRKLFPDEKQVVNPRAQMQRHLQEVRAGVSGFALTEQMNRLQQLVAQ
                     NEGVSLQSLSYDRSRDELRLSLRATSYAQMEQFRQQAQAYFQIPPGEMKQEKDHVEGQ
                     LTLRSQP"
     CDS             10803..11288
                     /transl_except=(pos:11031..11033,aa:other)
                     /note="Xxx"
                     /gene="outM"
                     /note="putative"
                     /citation=[2]
                     /product="secretory component"
                     /codon_start=1
                     /translation="MNELRRRWQVMSQRERLMALACGGLVVLCLLYYLIWAPWQESVR
                     QWQMTVERERQTVRWMQQQPPRFRRRKVRGGRXPVAISANGIGAQSAVRYGITVLRMQ
                     PQESQVSVTLARSDFNNLLHWLAELEQKNGVITQGIDVTAVPNSPGIVEVTRLSLERV
                     L"
     CDS             11472..12323
                     /gene="outO"
                     /note="putative"
                     /citation=[2]
                     /product="secretory component"
                     /codon_start=1
                     /translation="MDLIAFANTFPRVWLLALLLLGLIIGSFLNVVIYRLPLMLERSW
                     RQEARFHLGLPAGRPLARYDLCWPPSSCPHCHQRLRMRDNIPLLSWIWLRGRAHCCGG
                     AVSWRYPLIELLSGLSFLLAGLLWQPGLALLGALLCFGIFVALAAIDARTQLLPDVMT
                     LPLLWGGLLFNLADTFVPLEQAVVGAVAGYLSLWLIYWAFRLLSGREALGHGDFKLLA
                     ALGAWLGWQALPNLVLIASLTGLTATLLWQRIHRLSMQQPLAFGPWLAVSGAMGLVLN
                     VLGGWSH"
BASE COUNT     2786 a   3122 c   3876 g   2898 t
ORIGIN      
        1 gggtataggt aaaaagcggt acgttatttg ctgcggcacg gttttatttt tgtgtaagtc
       61 ggcaatatcg gtcatacagg aaatgggcat gtaaatagaa tgtgattttc ttgtatcgcg
      121 gaatattttc gctatgcccg acaaactgcg gcaatagcaa cgttacgggt tgcgtcgggg
      181 ttattggaaa atagtgggaa aaatggttgc tgaccggggg attgtcttgt cattaacctg
      241 agttatattg ttgatggttt tagtgctatc cggcacgatg atattgcgtg cttcttgcct
      301 ggcatatacc caaaataatt cgagttgcag aaaggcggcg acgcagtgaa tccccaggag
      361 cttacataag taagtgactg gggtgagcaa ggaaagccaa cgaacctaca acttgaagta
      421 tgatgggtat aaacaggatg cctgtcagcc agcaacacga ttgcagacag gtttagcgca
      481 ggcagtggcc gactgaaaac ggtcggctca tgaatatgat gatggcgcgt ttgtggcctt
      541 tccggcaagt cagtttgaaa gcgtcattcg ttgttcgtgc cggtgattta tagagtcgat
      601 gcggcgttaa gacgacaaca aatgcagaag tactattttt ttcttcgaaa atggtgctgt
      661 atggccctta ttccacgttt acggacgtgg caaggataat cggcgaacac atagggataa
      721 tgtttgcaga tgaatatctc aaaactgcca ccgttatctc cgtctgtcat cagacggatt
      781 ttattttatc tgttgatgct gctcttttgt cagcaactgg caatgatttt ctggcgtgtc
      841 ggcttgcctg ataattcgcc ggtcgccagt gtgcagatca ccccggcaca agcccggcag
      901 cagcccgtca ctctgaatga tttcacgtta ttcggcgtgt ctcccgaaaa aaatagatcg
      961 ggtgcgctgg atgcgtcgca aatgagtaat ttaccccctt ctacgctgaa tctttcactg
     1021 accggcgtga tggtgggcga cgataccgcc cgctccatcg ccatcatcag taaagacaac
     1081 gaacagttta gtcgtggcgt caatgaagag gttcctggtt ataacgccaa aatcgtatcg
     1141 atccggcctg ataaggtggt gttgcagtat caggggcgtt atgaagtgct gggactgtac
     1201 aaccaggaag aaaatagcgc agacggcgta tctggtgcgc agttaaacga gcaacttcag
     1261 cagcgtgctt ccaccaccat gagcgattac gtctcttttt ccccggtgat gaacgacaac
     1321 aaacttcatg ggtaccgatt gaatccgggg ccgaaaagcg attcgttcta tcgcgtgggg
     1381 ctgcaggata acgacatggc tgtggccctg aatggattgg atttgcgtga cgaagagcaa
     1441 gctaaaaaag ccatggagcg catggcggat gtgcataact ttaccctgac ggtggagcgg
     1501 gatggacagc gtcaggatat ttatatggaa tttgggggag acgagtaacg tgcttggcaa
     1561 agggataaaa aaaagttggg ggtggctggg cttaaccgtt ctcctgcttg gctcgccatg
     1621 tggctgggct gctgaatttt cagccagttt taaaggaacc gatattcagg agttcatcaa
     1681 caccgtcagt aaaaatttga acaaaacggt gattatcgac ccaacggtgc gcggtactat
     1741 cagcgtgcgc agttacgata tgatggacga aggacaatac taccagttct tcctcagtgt
     1801 ccttgatgtg tatggttttt cggtggtgcc gatggataac ggcgtcctga aagtcatccg
     1861 ttccaaagat gctaaatcgt cgtcgattcc tctggctaat aacgaacaac cgggcgtcgg
     1921 cgatgaactg gtgacccgtg tcgtgccgct caacaacgtg gcggcgcggg atttggctcc
     1981 gttgttgcgc cagcttaacg ataacgcggg tgccggtacg gtagtgcact atgaaccgtc
     2041 caacgtcctg ctgatgacgg ggcgtgcggc ggtgatcaag cggttggtcg atatcgtcaa
     2101 taccgtggac aaaaccggcg atcgtgaaat gatcaccgtg tcattgaatt acgcatcggc
     2161 ggaagatgtc gccaaattgg tgaacgatct caacaagacc gacgagaaaa acgcattgcc
     2221 aagcaccatg ctggccaatg tggtggcgga tggacgtacc aactcggtgg tggtcagcgg
     2281 cgaagaaaac acctccccat gcgcggtgga gatgatccgc caactggatc gcaagcaggt
     2341 ggcgcagggt gggactaagg ttatctatct taagtatgcc aaggcgttgg atctgatcga
     2401 agtactggcc ggtaatggga ccagcggtaa tcgtaattca tcgtcgacaa actcatcgcg
     2461 cccttcctca acgcgttcga gcagcacgtt aaacaatagt aactccagct catccggcag
     2521 tagttccggc agtggttcat cgtcatcatc ctccagttcc agtatgggat ttggttccgc
     2581 gtttggctcg gccaatagtt cgggcgggcg gacgattgtg atccagggca aagaggtgac
     2641 ggtacgcgct catgatcaga ccaactcgct gatcattaca cgtccgcccg atatcatgcg
     2701 tgatctggaa caggtgatta atcaactgga tattcgccgt ccgcaggtgc tggtagaggc
     2761 gatcatcgcc gagattcagg atgccgatgg cttgaatctg gggatccagt gggccaacaa
     2821 acgcgcaggg atgacccagt tcaccaatac gggcattccg atctctacgg caatgatcgg
     2881 aaccgatcag ttccgcagcg acggcacgct gacgacggcg tatgccagtg cattgagtaa
     2941 cttcaatggc attactgccg gtttttatcg tggcaactgg tccatgctgc tgacggcatt
     3001 gtccagcgac ggcaaaaacg acgtgttggc gacgccaagt attgtgacgc tggataacat
     3061 ggaagcgacc ttcaatgtgg ggcaggaagt gccggtgttg acggggtcgc aaacgacggt
     3121 tggttcagga gacaatattt ttaacaccgt agagcgtaag actgtcggca tcaaactgcg
     3181 ggtaaaaccg cagatcaacg aaggcgattc ggtgttgctg caaattgagc aggaagtctc
     3241 cagtgtggcg gaaggcaatg gcagcagcaa cagttccctg ggggtgactt ttaacactcg
     3301 tactgtaaac aacgccgtga tggtcaccaa tcgcgaaacc gtagtcgtgg gcggcctgct
     3361 ggataaaacc gccattgaaa ccaataacaa agtcccgttg ctgggcgata ttccgtggct
     3421 gggcagcctg tttcgttcga aaacccagac gatgagcaaa cgcaacctga tgctgttcct
     3481 gcgtccgacc attattcgcg acccccagca gtatcagcag gcatcgatta gcaaatacaa
     3541 ttctttcaac aacgagcagc aacagcagcg tggtcagggt aactcggtgc tggacaacaa
     3601 cactctgcgt ttatccggtg gcaataccta caccttccgg caggtgcaat cgtccatctc
     3661 cgcgttttat cagccggagg ggcgatgagc gatcagcccg ttcacacatc cgaactgagg
     3721 ccggtactgc cgtttgcgtt tgcccgggct cagcaaattc tgctgctgca ggatgagtcg
     3781 gcctcggcgg cggaggtggt ttgtgtccct gagacgcctg cgttggcgtt actggaagta
     3841 cgccgggttg caggggtggc gttgacggtg agccaggtca gcccggagga atttgaacgg
     3901 caattggtga tgcgttatca gcgcgactcc gaagaagcgc gccgcctgat ggaggatatc
     3961 ggtaacgata tcgatttcta tacgctggcc gaagagctgc cggacagcga tgatctgctg
     4021 gatggcgaag atgacgcgcc gattattcgg ttgattaacg ccatgcttac cgaagccatc
     4081 aaacacaagg cttcggatat tcatatcgaa acgtttgagc gccatttgct gattcggttc
     4141 cgtattgatg gcgtgttgcg ggaaatcttg cgcccgcagc gtcaactggc gtctttgctg
     4201 gtatcccgta tcaaggtgat ggccaaactg gatatcgccg aaaaacgtgt gccgcaggat
     4261 ggccgtatgg cgctgcgtat cggcgggcgg gcgattgacg tgcgtgtctc gaccctgccg
     4321 tccaactatg gtgaacgcgt agtattgcgt ctgctcgata aaaacagcgt ccggctcgat
     4381 ctggaaacat tggggatggc cgagcaccat cgccgtcagt tggatacgct gatccatcgt
     4441 ccgcacggca ttattttggt gaccggcccg acggcgtcgg ggaaaagtac caccctgtat
     4501 gcggcattga gcccgctcaa ttctgccgaa cgcaacatca tgacggtgga agatcctatt
     4561 gaatacgagc tggaaggcat cgggcagact caggttaacc ctaaagtcga tatgacattc
     4621 gcacgtggtt tgcgtgcgat cctgcgtcag gacccggacg tggtgctggt gggggaaatc
     4681 cgtgacggcg aaacggcgca aattgcggta caggcatcgt tgaccggcca tctggtgtta
     4741 tccacattgc ataccaatag cgcgttgggc gcattgtccc gtcttcagga tatgggcatc
     4801 gagccgttct tgttgtccac atcgctgctg ggagtgttgg cgcagcggct ggtgcgtacg
     4861 ctatgcccat catgtcgcca gccttacacc atcgatcacg agcaagcgga gcaaaccggc
     4921 ctggcggcag gcactacgct ctaccatccc ggcggttgtg aaaagtgtaa ttacagcggt
     4981 tatcgtggcc gtaccggcat tcatgaatta ctgctaatcg acgatacagt gcgcgccgct
     5041 attcatcgtg gcgaaagtga actgggtatc gcccggatgc tgggcgctaa gcgggtcacc
     5101 attcgtcagg atgggctgga caaggttctg gcgggcatca ccacctggga agaggtggtg
     5161 cgtgtaacga aagaggaatg acatggcgct gttccagtat caggcattaa atgcccaggg
     5221 aaaaaaaagt cagggcatgc aggaagcgga ttcagcccgt cacgcgcgtc aactgctgcg
     5281 tgaaaaaggg ttggtgccgg tcaaaatcga ggaacagcgc ggtgaggcgg ctccccgcag
     5341 tggtttttct ctgtcatttg gccgttcgca tcgtatcgcc agcgatctgg cgttgttgac
     5401 tcgtcaactg gcgacgttgg tggcggctct gccgctggaa gaagcgctgg acgcagtcgc
     5461 taagcaaagc gaaaaaccga aactgagcgc gctgatggcg gcggtacgcg ccaaggtcgt
     5521 ggaagggcat tcgctggccg aggcgatggg gaattttccc ggcagttttg agcgcttgta
     5581 ttgtgcgatg gtcgcggcgg gcgaagcgtc cggtcatttg gatgccgtgc tgaaccggct
     5641 ggcggattac accgaacagc gccacgagat gcgcagtcgg attcagcagg cgatgattta
     5701 tccctgcgtg ttgaccctgg tggcgatttc ggtggtatcg attttgttgt ctgccgtcgt
     5761 gccgaaggtt gtcgaacagt ttattcacat gaagcaagcg ctgccgttgt ccacccgttt
     5821 actgatgagc gccagcgatg cagtgagaac ctacgggcct tgggtggttc tattactggt
     5881 gcttgcgatc atgggatttc gcgtattact gcgtcaggaa aaacaccgcc tcgtttttca
     5941 tcgccgttta ttgttccttc ccgtcgtcgg gcgtgttgcc cgaggcctga acaccgcccg
     6001 ctatgcccgc accctgagca tccttaactc cagcgccgtg ccgctattac aggcgatgcg
     6061 tatcagtggc gatgtactga ccaacgatta tgcccgtttt cggctggggc aagcgaccga
     6121 tgccgtgcgt gaaggcgtga cgttgcataa ggcgctggag caaaccgcgc tgttcccgcc
     6181 gatgatgcgc cacatgatcg ccagcgagcg caggcgagct cgacggcatg ttaacccgcg
     6241 cggcgataac caggatcgtg aattcagcgc tcaaatgacg ctggtgctgg ggctgttcga
     6301 acccctgctg gtggtgagca tggccggcat tgtgctgttt atcgtgctgg cgattctcca
     6361 gccgattctg cagttgaata ccctaatgag tatgtagcga agattgatac gttaatgagg
     6421 taacgaaaag atggaacgac gtcagcgagg ttttaccctg ttggaaatta tggtggtcat
     6481 cgtcattctc ggtgtactgg ccagtctggt tgtcccgaac ctgatgggca acaaagaaaa
     6541 agccgacaag cagaaagccg tcagcgacat agtggcgctg gagagccaac tggacatgta
     6601 caaactggat aacagccgtt acccgacaac cgagcagggg ctgggagcac tggtgaaaaa
     6661 accgaccaca ccaccggagc cgcgtaatta tccgcaggat gggtatattc gtcgtttacc
     6721 gcaagacccg tggggcgcgg aatatcaact ggttagccca ggccgccacg gcaagataga
     6781 cgtgttctcc tatggcccgg acggcatgcc ggataccgat gacgacatcg gcaactggaa
     6841 tgtcggcaac ggcgcgcaca acaatggcgg caacggtaac gggaatccct gatcgtgcgt
     6901 cagcggggat tcacgctgct ggaaatcatg ctggtggtgt tgctggccgg tgtggccgcc
     6961 acattggtaa tgatggcgat tcctgcgccg aagcagcagg atagcggctg gcagatcgcc
     7021 cgcttcaaag cgcaattgca gtatgcggta gaggacagcc agatgaatga ccatattctg
     7081 ggcatttata ttcagcctca ccgctggcaa tacgccttgc tacagcgtca ggtggtggag
     7141 aattcgccgg aagaacaaca gcgtctgcgc tatgtctgga taccgtggca accgtaccgc
     7201 atgtcggttc ccagcgagtt gccggacagc tttcacatcg agctgtctac acaagtcggg
     7261 gccgcgggag acggcaccgg tttttcccca ggcaacggcg atcctcatgt tctgattctg
     7321 ccgggcggcg aagtgacgcc atttcgtctg accttgcgta atggcaacga ttccgcctgg
     7381 ttacaggtgg ataccaacgg tcaggtgcac acgtcgccgg aagcggagca gaagggatga
     7441 aacaacaggg aatgacgttg cttgaggtga tggtggcact ggtcatcttt gcgctggcgg
     7501 gtttgacggt gctgaaaacg acggctcagc aggcgaatgg gttggggcgc ctggaagaga
     7561 agaccttcgc actctggatt gcagaaaatc aacaggcggc gatgcggctg gaaaaacagt
     7621 ggccgcagag ccaatgggtc gatggtgaag tgaatttcgc cggttcactg tggttttggc
     7681 gtattcaggg catcgccacg gcggatacgc gggtgcgggc aattgatgtt gaagttcgtc
     7741 atgatcgcga cagccgggcg gcggatgccg tcttgcgcag ttatctggtc cagcccggag
     7801 aacctgcgca gtgaagcagc ctgaacgcgg ttttaccctg ctggaagtga tgctggcgct
     7861 ggcgattttc gctgcgttga gtctggctgc ctcgcaggtg atgaacggtg tgatgcgcaa
     7921 tgacgaggcg tctgcgcgta aagaggcgcg tctggccgag ttgcaacgcg gtttttcctt
     7981 gatggagcgg gatttttcac aaatcgttcc gcgccgcagc caagggtatg agctggggtt
     8041 ttacgccgag cgttatcaat taagcagtgc cgattgggcc gtcagtttta ttcgcaacgg
     8101 ctggctcaac cctctgggga ttatgccgcg ttctgaatta cagcgggtgg gataccggct
     8161 acgcggcgat acgctggagc ggctgtttta cgattcctcg gaaccgctgt cgacgcagga
     8221 accggcggta cgcccggtgt taaccggcgt gacggggttt gttctgcgct ttttcggtaa
     8281 ggacggctgg caggagcgtt gggatgaccc ggccaatctg ccgcaaggta tcgctatcgt
     8341 tgttacctta cgcgattacg gcgagattac ccgtatattc ctgatcacgc cgcgctacgg
     8401 agccgcaacc caaaggcggt ccgaacgata ataacagcga cgaaaataag gaccaacaga
     8461 atcaggagca gcaaccacag gaaaataacg attcgtccgg cgatcagagt caggcctcgc
     8521 cggatgataa cggcggaggt gactcgtgag tcgtcggcaa cggggtgtcg ctttgttgat
     8581 cgtgatgctg atgctgtcgt tgatggtcac gattgcggcg tctattaccg agcgcagcgg
     8641 caaagcgtgg cagcgtacca gcaatctgtt gaatcgtacc caggcgcgtt ggtatgcatt
     8701 aggcgcagag gcgttgattt ccaacgtatt gcagcgtgac gcgcaggcct cgccggagag
     8761 tacctttatc ggtcagccct ggtcaaaagt ggatcaccag atgatggcgg acggcactga
     8821 aattcgtgct caggcgctgg acgggcaggc atgtctcaat ctgaacgcgt tgagcccggc
     8881 tagaaacgtg acgcccaata acgcatccgg taataacaca tccggtaata acaatgcggc
     8941 taacggcagt agcggcaacg gtaattcgcc tcagccgccc aaagtcggca cttctgagca
     9001 agtgccttat gcggcacagg tttttcggca gttaatgatc gtactggggg aagaccctaa
     9061 gcaggcggag cgtataaccg acgccttacg ggattggctc gacgaagata gcgaaccgtt
     9121 gatgaatggg gcggaggatg acagctatgt caattttcat cccggtaatc agcgtatgac
     9181 ggacgttacg gaattacggg cggtcatggg catggatgcg gcgttgtacc gccggttatt
     9241 gccttacgtt tgcgtgttgc cggtggataa actggcgatt aacgtcaaca ccctgatgcc
     9301 gggcagcgcg ccgctattat cggcgttatt tatgggcgat atcagtctgg atatggccga
     9361 gcgtattttg caacaacgac cgccgcaggg atggcgcaat cttaatgatt ttatgggaat
     9421 gtcggcgctg ccggaaagcg gcaaaaatgg cgctcgtcag gtgttggtta tcaagagcga
     9481 ctggttcttc gccgacattc agattcgggt ggacgacagt gaattctatc aacgcagtct
     9541 gtttcatcgc ggtaaacaga tcgaggtggt acaacgtcag tacgggggat ataggacggt
     9601 gaacccatga gtaaggcaga gaataccagc ggtaaacagc aactgattct gcggctgagc
     9661 gcagatactt cagatagcct ggaatggtta atctggtccg tcagccgcca tcaaaccctg
     9721 acgacaggca gcggaacgct ggaaagcctt caggcggtgt tggcggatta tccggtgata
     9781 tcggcacgag tactggtgcc gtcaaccgat gtgacatttc ataccttgtc tttgccgcgt
     9841 caatcacgcc ggcaattatt acaggccatt ccattcatgc tggaagagca ggtggcgagc
     9901 gatattgacc aactgcattt tgccgttatg gatatgcatg gggataacgc gacggtggcc
     9961 gtggtgcaga aatcgcgatt gcgggcatgg ctgaatcaat gcgagacgct gggcgttccc
    10021 gtagagacgg tggtgccgga tgtgatggca ttgccgcgag cggacagcgc ctggagcgcc
    10081 atcagccacc ggaatctgtg gttgtttcgg ctggattcgg gtatcggtat ggcggcggag
    10141 gagaactggt atcagtcgct gctggccttt cagccgctgc ctgccgtaca ctgttatagc
    10201 ccggtacccg cgtcggcact cacctggcag cctcagccgg tcacggattt attgacgctg
    10261 gcggctcagg tgaacttgtc gatgagtatg gatttgcggc aaggggagta tgcgccggtg
    10321 aagccctgga aacaggcgtt gttaccgtgg cgcaatgtgc tgatcgcgct gtccgcctgg
    10381 ctgttgctgg tgctggggga atccgtctgg acgcattatc agtggtatcg ccaggcggat
    10441 tactggcgtc aggagagtgt gcgggtgtat cgcaagctgt ttccggatga gaaacaggtg
    10501 gtgaacccac gcgcacagat gcagcgtcat ttacaggaag tgcgtgccgg cgtcagtggt
    10561 ttcgcgttaa ccgaacagat gaaccggctg caacaactgg ttgcgcagaa tgaaggcgtt
    10621 tcgttgcagt cgctgtcgta cgatcgcagt cgtgatgagt tgcgcctcag cctgcgtgcc
    10681 acctcctatg cgcagatgga acagttccgc cagcaggctc aggcatattt ccagatcccg
    10741 ccgggtgaaa tgaagcagga aaaagatcat gtggaaggcc agttaacctt aaggagtcag
    10801 ccatgaatga attgcgtcgt cgctggcagg taatgagcca acgcgagcgg ctaatggcac
    10861 tggcgtgtgg cggtctggtc gtgttatgcc tgttgtatta tctgatctgg gcgccctggc
    10921 aggagagtgt gcggcaatgg caaatgactg tcgaacgtga gcgccagaca gtgcggtgga
    10981 tgcaacaaca accgccgcga ttccgccgcc ggaaggtgcg cggcggcaga tagccggtcg
    11041 cgatatcagc taacggtatt ggtgcgcaaa gcgcggtccg ctacggcatt accgtgttgc
    11101 gtatgcaacc gcaggagtcg caggtgtcgg tgacgctggc ccgtagcgat ttcaataatc
    11161 tgctgcactg gctggctgaa ctggagcaga aaaacggcgt catcacccaa gggatagatg
    11221 tgacggccgt tcccaacagc cctggtatcg tagaagtcac ccgcctgtct ctggaacggg
    11281 tgctgtaatc ggcgcaatgg ctgtcctgcc ggatatcagg caggcgcatc gcgtagcgcg
    11341 aagccgtcat cgataatcag ccagtctgcg cgtggcgcgc aaaaaacgac gcccgttgcc
    11401 tgcctgtctg gcaggggcga atatattgtg tcagcatacc gggtcatcag ggatcggaat
    11461 gggagaacga catggatctg atcgcctttg ccaacacatt cccgcgtgtc tggctgctcg
    11521 cactgttgtt gctgggcctg attatcggca gttttctcaa cgtggtgatt tatcggctgc
    11581 cgctcatgct ggagcggagt tggcggcagg aggcacgttt tcaccttggt ctaccggctg
    11641 gcaggccgct tgctcgttat gatctttgct ggccgccttc ttcctgtccc cattgccatc
    11701 agcgactgcg tatgcgcgac aacattccgt tgctgagctg gatatggctg cgtgggcggg
    11761 cgcattgttg tggcggcgcg gtgtcatggc gttatccgct aattgaattg ctgtccgggt
    11821 tatcgtttct gctggccggc ttactctggc aaccggggtt ggcgttgctg ggggcattgt
    11881 tgtgttttgg catattcgtg gcgctggcgg cgattgacgc ccgaacacag ttactgcccg
    11941 atgtgatgac attaccgcta ctatggggcg ggttattgtt taacctggcg gatacctttg
    12001 tgccgcttga acaagctgtt gttggtgccg tggccggtta tctgtcgctg tggctgattt
    12061 actgggcatt tcgtctgctg agcggacgcg aagcgctggg acatggcgat tttaaactat
    12121 tggcggccct gggagcctgg ctgggctggc aggcactgcc caatctggtg ttgattgcct
    12181 cgctgaccgg tctgacggcg acgttattgt ggcaacggat acaccgcctc agcatgcagc
    12241 aaccgctggc gtttggcccc tggctggcgg tgagtggcgc gatgggtctg gtgctgaatg
    12301 tgctgggcgg ctggagccat tgaacgggtg atgttcggtg tgcgccgcct gattaccggg
    12361 gcggatcgtt atgctaaacg gcacaccctc gcattcacag cttcgtgagt tgttgattat
    12421 tgtgaccggc tttttgctat aacttgctat aaaaaagaaa caggctggca agaagggctg
    12481 ccagcctgtc gtttattcgc ctttcgtgta ctgataatta ttttttgttg ttgcttcggc
    12541 aggagccttc tgccgaagtg gatgaggaaa caccgtcagg gttattccag catcagtaaa
    12601 aaagtgccta cccacaaaaa cagaatgaat gaaacgccca tgaataagta cttcactgat
    12661 ttctctccag caatatcacg cc
//