From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Streptococcus mutans beta-fructosidase (fruA) gene, complete cds.
Date: 29 Dec 92 01:21:20 GMT
Distribution: bionet
Checksum: 54603 10

LOCUS       STRFRUA      5010 bp ds-DNA             BCT       07-DEC-1992
DEFINITION  Streptococcus mutans beta-fructosidase (fruA) gene, complete cds.
ACCESSION   L03358
KEYWORDS    beta-fructofuranosidase; beta-fructosidase; fructan hydrolase;
            secreted protein.
SOURCE      Streptococcus mutans (strain ) (library: lambda GEM11, lambda
            JECP1,2, and 5) DNA.
  ORGANISM  Streptococcus mutans
            Prokaryotae; Firmicutes; Firmibacteria; Gram-positive cocci;
            Streptococcaceae.
REFERENCE   1  (bases 1 to 5010)
  AUTHORS   Burne,R.A. and Penders,J.E.
  TITLE     Characterization of the Streptococcus mutans GS-5 fruA gene
            encoding exo beta-D-fructosidase
  JOURNAL   Infect. Immun. 60, 4621-4632 (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     -35_signal      607..613
                     /gene="fruA"
     -10_signal      636..641
                     /gene="fruA"
     RBS             671..676
                     /gene="fruA"
     CDS             685..4956
                     /gene="fruA"
                     /product="beta-fructosidase"
                     /codon_start=1
                     /translation="MEEETVCKNWFMRKSGKSWIFGCAVFFVLGLATALPVAAEEISQ
                     TTAADTAVTEVRTEDSSQTSSQETAVTETTQSEETASKQLTTPAVADQTTEPTDNEPI
                     SSSDGASSPYQVTDTTEPQQTLTPADSEPQAKADVQQAAAPKKEEINPVTNLEDMSHD
                     TNGTWEVREDGIHSNAIGKGDSFLYSQSSGKNFVYATDVTFKQNSGAAALVFRSNNDS
                     NNKNMYAVNVDIGGHKAKFWRWVDNKDIQLIDERDVVPTADNRYTLKVVAVNNWISYY
                     VNDVLMASTGDYVLQKADKGQNTVIPEGHFGLLNWNGDMVFQNTKFALLDDTTAPLID
                     NITVRSDRGNVEKQGQFFSEEPLHIQYVSNDASQVSLDIAKHNPAATVTVEDKTGRVY
                     TDPSHLPVNVGANYFTVKSTVIDSFGRTVTLTYRINVHRRQNDEVYYNELYRDQYHYS
                     VKDGWANDPNGLVYYNGVYHLFHQFYDDTKWGPMHWAHATSTDLIHWKEEPIAFYPDS
                     NGYMFSGCVVVDEHNSSGLFKTAKGGLVAIITANGNGQRMELAYSEDEGKTWQKYDRI
                     VADWSNDPLQNQDFRDPKVFHWNNQWFMVLAGGPLRIYSSNNLKDWKVESTYPDLHTE
                     CPDMYPIVANDGVLKWVLSRGGRFYKVGDFKQVDGKWTFIADDAYKDKDQVMNFGKDS
                     YAAMTYYVHDFGTETRPTIPKLTEVNWMNTWEDYCNLVADTVGQDFNGTFNLNLDLGL
                     INENGQYILTQTPVKAYDSLRDVNTALHFKDVTVDANNTLLKDFKGDSYEIVSHFRPD
                     EKTTKVGFNLRVGNGQATKVIYDLQTETLSIDRSQSGTILSAAFAKVNSQHVTKNADG
                     SIDLHIYVDRASVEVFSKNNTVAGANQIFPNPEAVGASIIVEGGKAQADISVYQMKTI
                     WTDKKDTAKPVAMNTTTAKELALQVGQTQDLQVYLAPASVRQDVEWTISDPSLVRTSQ
                     KGNVLHLTAVKKGKLTITAISKENPSLSKIFTISITLNNFKTNLKGLQSVTGKWYVDD
                     ETLYDSNTSSNDYYMASQKPGFKEYDYDIDLKYQRGLINLFVASGNIDPSQAYSVQFG
                     DSETVRLYRFAGDTIAEANMGKRINDDQYHHIKVTKTKNSIIISVDGQEVMSHNFDQV
                     DSYFNDAYVGLGLWDGAVEFQNFFVTDHATTPKPDSDPTPQPDAPEALAQERELIDPA
                     TGVRVILQKGELASIVRVKVSHIETNDAHTPAVLNAKDYDLFNITPIDKNEKVVAITK
                     PATVLLPIDAGKVVDKVVYLPNTDKEESLPFTIVSLTDSNGKKQSYVRFTAEHFSEYG
                     LVYQAENQTNLKSKEKQDNVAISYPLNLEQEVKVSSISRKYAANKTADVNSVQQTEPS
                     VMSSSSKATLPDTGDHKTDLSQLGVLAMIGSFLVEIASYFKKSKD"
BASE COUNT     1607 a    880 c   1043 g   1480 t
ORIGIN      
        1 ggatcctaac tatgcagaca agttaatagc tgttatagag gaaaataatt taacagatta
       61 tgataaatag gtattctaaa aaatggttga gtttattagt tttggataaa gatatctgac
      121 tgatgaaaca gcatcgttta gaatgctttt tttaattgtt ttcaagctat attcttcaac
      181 aatcaaaacg attgttgtct tgatactaac gttcgctgtc tccgtttccc acctcaaagc
      241 aaaaggtttt ccagatcttt gagtttcctc tgctctccaa tattgtttta taggtggttg
      301 tgatatgtgt ttgagcagtc aataataaga tctaatgaga cagtcagact tgtcactagc
      361 ggctaccatt aattaatgag acacttagca cctattaact ttgccgcctt gcctaatcta
      421 tttttttttg atttttattg agcattaatg acatctgtca tattaaggaa atgacaattg
      481 ctagatggaa gatagatagc gatttggtat actgaggata agatagcgct tacaacattt
      541 taaataaatt tttgaaactt aaatactgac cgacctaaat aggaaagaaa tgaggtatta
      601 aaaattttcg agcagccaat ttttatggct gtctgtttaa ttagagtttg tcagtttcat
      661 aaatttgaaa aggagagcaa ggaaatggaa gaagaaacag tttgtaaaaa ttggttcatg
      721 cgtaaaagtg gtaaaagctg gatttttggc tgtgccgtct tttttgtgtt aggtttggca
      781 actgctttac cggtggcagc tgaagaaata agtcaaacaa ctgctgctga tactgctgtt
      841 acagaagtaa gaacagaaga cagcagccag acgtcatctc aagaaaccgc agttacagaa
      901 acgactcaat ctgaggaaac tgcttcaaaa caactgacaa ctccggcggt agcagatcaa
      961 actacagagc ctactgataa tgaacccatc agttcctctg atggtgcgag cagtccttat
     1021 caagtgactg acacgacaga acctcaacaa actctcactc ctgctgactc tgaacctcaa
     1081 gcaaaggcgg atgttcaaca agcagcagct cctaagaaag aagagatcaa ccctgtgacc
     1141 aatttagaag atatgtcaca tgatacgaat gggacttggg aagtacgaga agatggtatt
     1201 cattctaatg ccattggtaa gggtgattct ttcttgtatt ctcagtcaag tggtaagaat
     1261 tttgtctatg caacagatgt tacttttaaa caaaacagcg gtgcagcagc tcttgttttt
     1321 agaagtaaca acgatagcaa taacaaaaat atgtatgcgg tcaatgtgga tataggcgga
     1381 cacaaggcta agttttggcg ttgggttgac aataaagaca tccaattaat tgatgaacgt
     1441 gatgtggtac cgacagctga taaccgctat actcttaagg ttgtggcggt taataactgg
     1501 atttcttatt atgtcaatga tgttttgatg gctagcacag gtgattatgt ccttcaaaaa
     1561 gctgataaag gacaaaacac tgtcattcct gagggacact ttggtctttt gaattggaat
     1621 ggcgatatgg ttttccaaaa tacaaaattt gccctattgg atgatacaac agctccgttg
     1681 attgataata ttacagtaag gtctgataga gggaatgtcg aaaaacaagg ccaattcttc
     1741 tctgaggaac cccttcatat tcaatatgtt agcaatgatg cttctcaagt gagcttggat
     1801 attgctaaac ataatcctgc agctactgtc acagtagaag ataaaactgg tcgtgtttac
     1861 acagatcctt ctcatcttcc agttaatgta ggagctaatt atttcactgt taaaagtaca
     1921 gttattgatt catttggtcg tacagtaacc ttgacttatc gtattaatgt ccaccgtcgg
     1981 caaaatgacg aagtctatta taatgaactt taccgcgacc aatatcacta ttctgtaaaa
     2041 gacggctggg ctaatgatcc taatggcttg gtttattaca atggtgttta tcatctcttc
     2101 catcaatttt acgatgatac gaaatggggc cctatgcact gggcgcacgc gactagtacg
     2161 gatttgattc actggaaaga agagccaatt gctttttacc cagattcaaa cggctacatg
     2221 ttctcgggct gtgtagtggt tgacgaacat aacagttcag gcttatttaa gacggctaag
     2281 ggtggtttag ttgcgattat cacagctaat ggaaatggac agcgcatgga acttgcctat
     2341 agtgaagatg aaggcaagac ttggcagaaa tatgacagga ttgtagccga ttggtcaaat
     2401 gacccattgc aaaatcagga ttttcgtgat ccaaaagttt ttcactggaa taaccaatgg
     2461 ttcatggtac tagcaggggg tccgcttcgc atttattctt ctaacaattt gaaagattgg
     2521 aaagtggagt ctacttatcc tgatcttcat acagaatgtc cagatatgta tccaattgtt
     2581 gctaatgatg gtgtgcttaa atgggtgttg tctcgcggcg gtcgttttta caaggttggt
     2641 gatttcaaac aagtagatgg taaatggacc tttatagcag atgatgctta taaagataag
     2701 gatcaggtca tgaacttcgg taaagattct tatgccgcca tgacctacta tgtacacgat
     2761 tttggtacag aaacccgacc aaccattccc aaattaacag aagtcaactg gatgaacacc
     2821 tgggaagatt attgtaatct tgtagcagat acagttggac aggactttaa cggtacgttt
     2881 aatttaaacc ttgatcttgg ccttatcaat gaaaacggtc agtacatctt gacgcaaacg
     2941 cctgttaagg cctatgatag tcttcgtgat gtgaatactg ccttgcattt caaagatgtg
     3001 acagtagacg ctaataatac tcttttgaag gacttcaaag gagacagtta tgaaattgtt
     3061 tctcatttcc gaccagatga gaagaccaca aaagtaggct tcaatcttcg tgttggcaat
     3121 ggtcaagcaa cgaaagtgat ttacgacttg cagacagaaa ccttgtctat tgaccgtagt
     3181 cagtcgggta ctatcttatc tgctgctttt gcaaaagtta atagtcagca tgtgactaaa
     3241 aatgcagatg gctccattga tttgcatatt tatgttgatc gtgccagtgt tgaagtcttt
     3301 tccaaaaata atacagtggc tggtgctaat caaatttttc ctaatccaga agctgtagga
     3361 gccagtatta ttgtagaagg cggcaaagct caagcagata tctctgttta tcaaatgaaa
     3421 acgatttgga cagataagaa agatacggca aaaccggtgg ctatgaacac aacaactgcg
     3481 aaagagctag cccttcaagt tggtcaaact caggatctgc aagtctatct ggcaccagca
     3541 agtgttaggc aagatgtgga atggacgatc agtgatccaa gtcttgttag gacaagtcaa
     3601 aaaggtaatg ttcttcattt gaccgctgtg aaaaaaggaa agcttaccat tacagcaatt
     3661 tctaaagaaa acccaagtct cagtaaaatc tttacaatca gtatcacctt aaataatttc
     3721 aagactaacc tcaaaggttt gcagtctgtt actggtaagt ggtatgttga tgatgaaacg
     3781 ctctatgata gtaatacaag ttcgaatgat tactatatgg cttctcaaaa accgggtttc
     3841 aaagaatatg attacgatat tgatctcaaa tatcaacgtg gtttaattaa tctttttgtt
     3901 gcttctggca atattgatcc gagtcaagca tattctgtac aatttggtga cagtgagact
     3961 gttcggcttt accgttttgc tggtgatact attgcagaag ctaatatggg taaacgaatc
     4021 aatgatgatc aataccacca tattaaagtc acaaaaacaa aaaatagtat aatcatatca
     4081 gtagatggtc aagaagtgat gagtcataac tttgatcagg tggactcata ctttaatgat
     4141 gcttatgtag gtctcggttt gtgggacgga gctgttgaat tccaaaactt ctttgtaaca
     4201 gatcatgcga ctactcccaa accagattct gatccgacac cgcaaccaga tgcaccagaa
     4261 gcattggctc aagaaaggga attgattgac cctgcaactg gcgtccgtgt cattcttcaa
     4321 aaaggggagc tagcttctat cgttagagtc aaggttagcc atatcgaaac gaatgatgct
     4381 catacaccag ctgttctaaa tgcaaaagac tatgatcttt tcaatatcac acctattgat
     4441 aaaaatgaga aagttgttgc tattacgaaa ccagcgacag tcttattgcc aattgatgct
     4501 ggaaaagtgg tagataaagt ggtgtattta ccaaatacgg acaaagaaga aagccttcca
     4561 tttacgattg tgagtttgac agatagcaat ggtaagaagc aaagttatgt tcgctttaca
     4621 gcagaacact ttagtgaata cggcttagtc tatcaagcag aaaatcaaac aaaccttaag
     4681 agtaaagaaa agcaagacaa tgttgctatt tcttatccat taaatttaga acaagaagtt
     4741 aaggtttcat ctatttcacg aaaatatgct gcaaataaga cagcagatgt caatagtgtt
     4801 caacaaacag aaccttcagt aatgagttct tcatctaaag caacgctgcc agatactggt
     4861 gatcataaga cagatttaag tcaattagga gtgttggcaa tgattggctc atttcttgta
     4921 gaaatagcta gctatttcaa aaaaagcaag gattgattaa agtaaacctc tagattttga
     4981 cgcgtctaga ggtttgtttt tggctaacaa
//