From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Plasmodium knowlesi erythrocyte binding protein (beta) gene,
Date: 22 Aug 92 10:01:19 GMT
Distribution: bionet
Checksum: 05376    10

LOCUS       PFAERTHBPB   4762 bp ds-DNA             INV       22-AUG-1992
DEFINITION  Plasmodium knowlesi erythrocyte binding protein (beta) gene,
            complete cds.
ACCESSION   M90694
KEYWORDS    erythrocyte binding protein.
SOURCE      Plasmodium knowlesi (strain Malayan H) late schizont DNA.
  ORGANISM  Plasmodium knowlesi
            Eukaryota; Animalia; Protozoa; Microspora; Microsporea;
            Microsporida; Haemosporina; Plasmodiidae.
REFERENCE   1  (sites)
  AUTHORS   Adams,J.H., Hudson,D.E., Torii,M., Ward,G.E., Wellems,T.E.,
            Aikawa,M. and Miller,L.H.
  TITLE     The Duffy receptor family of Plasmodium knowlesi is located within
            the micronemes of invasive malaria merozoites
  JOURNAL   Cell 63, 141-153 (1990)
  STANDARD  full staff_review
REFERENCE   2  (sites)
  AUTHORS   Fang,X., Kaslow,D.C., Adams,J.H. and Miller,L.H.
  TITLE     Cloning of the Plasmdium vivax receptor
  JOURNAL   Mol. Biochem. Parasitol. 44, 125-132 (1991)
  STANDARD  full staff_review
REFERENCE   3  (bases 1 to 4762)
  AUTHORS   Adams,J.H., Sim,B.K., Dolan,S.A., Fang,X., Kaslow,D.C. and
            Miller,L.H.
  TITLE     A family of erythrocyte binding proteins of malaria parasites
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 89, 7085-7089 (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     CDS             join(585..644,827..4027,4128..4207,4440..4560)
                     /product="erythrocyte binding protein"
                     /gene="beta"
                     /codon_start=1
                     /translation="MEGKKKRPLFFLLVLLLSHKANNVLFERMKGILLLECENEYVKN
                     ENGYKLATGHHYMDNDQIERWLQGTDRSRRVKIEENVKYKYNVEELNTKYEQTKGKRI
                     NRILKESTYEAQNVADNNYIDDKANGEYKTDNKTNKGEGARNMVMLDYDISGSGHPDG
                     IIDNVVELGTEDEGNFLENSSKGGDHPYRMNRKERMCSGVINQTFLQKNVMRRCNNKR
                     KRGTRDWDCPTKKDVCIPDRRYQLCMKELTNLVNNTKTHSHNDITFLKLNLKEKLTYD
                     AAVEGDLLLKKYNNVYSEDLCKDIKWSLEDFGDIIMGTDMEGIGYSQVVENNLRTVFG
                     TGTKTQLDRKKWWNESKKYIWEATILSVKKKLNGYSAWNCKEDVQINVEPQIYRWIRE
                     WGMDYMSELPKEQRKIKEKCDRKLYYTNLRICTMSPCNDSCKLYDQWITRKKKQWDVL
                     STKFSSVKKGQIIETENITTAYDILKQELNGFNEVMFENEINKRDNVYIDICLCAADE
                     PNKNTQEHLKKLKSAPKLETQRSHSTIQPMSSSGAEKVQGDLAHGNINDAAYKSTTDE
                     AAKGDGQNGNQTVAESNIKGTDNIENEAAKNVDTYKFVTERSADTRGATDITETGEEK
                     LNTSYSGSSEITVKENIPGDGIVKDVSAAVENSENPLETKHKIFEPSKDNSDNSENSG
                     SMEFKATSSNPITEAVESSSAEGQVQEDSAHRSVNTGRDNSTISAATSDDGLSSGDKR
                     VESLTSIENADDGGDPVQGSLWNLLNDPSVGAGGGKSHIKTEENEGSQAEIDGKNVDI
                     AEQRTATITEVQPERPDLSDTDNGNVPRSGNKQNEGATALSGAESLESNESVHKTIDN
                     TTHGLENKNGGNEKDFQKHDFMNNDMLNDQTSSDQTSSDQTSSNQTSSDQTSSNQTSS
                     DQTSSDQISSDQTSSDQTSSNQTSSDQTIDTEEHHRDNVRNPEIKSSEDMSKGDFMRN
                     SNSNELYSHNNLNNRKLNIDQYEHRDVKATREKIILMSEVNKCNNRASLKYCNTIEDR
                     MLSSTCSRERSKNLCCSISDFCLNYFELYPYEFYNCMKKEFEDSSYECFTKGSSTGIG
                     IVYFATGGAFLIILLLFVSKNVASNDYEEEATFDEFVEYSDDIHRTPLMPNHIEHMQQ
                     FTPLDYS"
     intron          586..643
                     /number=1
                     /cons_splice=(5'site:yes,3'site:yes)
                     /gene="beta"
                     /evidence=EXPERIMENTAL
     exon            827..4027
                     /number=1
                     /function="erythrocyte binding domain"
                     /gene="beta"
     intron          4028..4127
                     /number=2
                     /cons_splice=(5'site:yes,3'site:yes)
                     /gene="beta"
     exon            4128..4207
                     /number=2
                     /function="transmembrane domain"
                     /gene="beta"
     intron          4208..4439
                     /number=3
                     /cons_splice=(5'site:yes,3'site:yes)
                     /gene="beta"
BASE COUNT     1838 a    623 c    970 g   1331 t
ORIGIN
        1 acggagtaat atttctaatt gtttaagata aaggggaatt tttctacggt aactattcac
       61 atttctactt ttttttttat tagatgaaaa cagaaaaaat agaaatgttg gattggtggg
      121 aacgtaaaaa aaatattggt aatggttaaa aaaaaaaaga agagtactat aggtgttgca
      181 tgaatgtgta cgcgtaaagt gccttgttct aatgaaaaaa aagaagagta ctaaaggtgt
      241 tgcattaatg tgtacgcgta aagtaccttg ttctaatgaa agaaaaaaaa aaaaaattaa
      301 aaaaagaggt tttagaaata gcaataagaa attgtaataa agaattaccc acaacagagt
      361 cacaacattt gcgatgtttt acaccctata tatgtaggtt catataattt agttctgggt
      421 atttttcttt ttcgtcttct ttttatatcg gtgcgttcaa atttgttctg tgcttttaca
      481 tgttttgtta tatgtgtaga acacctagct acgtaatttg cgcatacacc ccccttaata
      541 tgctttgtat aatataaaca cgtatacact tatttttaaa tacaatggaa ggaaaaaaaa
      601 aacgcccttt attttttctc ctagttttat tattgtcaca caaggtaatg aaggacaatt
      661 ttttttatca gactaataaa ttatcatgaa ggaaaaaaaa agaaaaaaaa aagaaaaaaa
      721 aaaaaaaaaa gggggaccat ttaattatag taaaagaagg gggcacaaaa tgaagaaagc
      781 taagaatcgt gatttgtgta atcagttctt tttctttttc ttttaggcga ataatgtatt
      841 attcgaacga atgaagggaa tccttttact agaatgcgaa aatgaatacg tgaaaaatga
      901 aaatggttat aaattagcta caggacatca ttatatggac aatgatcaaa tagaacgatg
      961 gttacaagga actgatagaa gcagaagagt gaagattgag gaaaatgtaa aatataaata
     1021 taacgtagag gaactaaata caaaatatga acaaacgaaa ggaaaaagaa ttaatcgcat
     1081 tttgaaggaa tccacttacg aggcgcaaaa tgttgcagac aacaattata tagatgataa
     1141 agcaaatgga gaatataaaa ctgataataa aactaataag ggggaaggtg caaggaatat
     1201 ggtcatgttg gattatgata tatctggcag tggccaccca gatgggatca ttgataatgt
     1261 tgttgaattg ggaactgagg atgaggggaa ttttcttgaa aattcctcaa aaggcggtga
     1321 ccatccttat cgtatgaatc ggaaggaaag aatgtgtagt ggtgttatta atcaaacttt
     1381 tcttcagaag aatgtaatga ggaggtgtaa taataagaga aaacgcggga caagagattg
     1441 ggactgtcca actaagaagg atgtttgtat accagatcga agatatcaat tatgtatgaa
     1501 ggaacttacg aatttggtaa ataatacaaa gacacattct cataatgata taacatttct
     1561 gaaattaaat ttgaaagaga aacttactta tgatgctgct gtggagggcg atttattatt
     1621 aaagaagtat aacaacgtat atagcgaaga cttatgtaag gatattaaat ggagtttgga
     1681 ggattttgga gatataatta tgggaactga tatggaaggt attgggtatt cccaagtagt
     1741 ggaaaataat ttgcgcacag tctttggaac tggtacaaaa acccaactgg atcgtaagaa
     1801 atggtggaat gaatctaaaa aatatatttg ggaagcaacg attttatcag ttaaaaaaaa
     1861 attaaatggg tattctgcat ggaattgtaa agaagatgtt cagataaacg tggaaccaca
     1921 gatatataga tggattcgag aatggggaat ggattatatg tcagaattac ccaaagaaca
     1981 acggaaaata aaagaaaaat gcgataggaa attatactat actaatttaa gaatatgtac
     2041 aatgtctcca tgcaatgatt cctgtaaatt atatgatcaa tggataacaa ggaaaaaaaa
     2101 acagtgggat gttttgtcaa caaaattttc aagtgtaaaa aaggggcaaa taatcgagac
     2161 ggaaaatatc acaacagcat atgatatact aaaacaggaa ttaaatggat ttaatgaagt
     2221 gatgtttgag aatgaaatta acaaacgtga taatgtctat attgatatat gcctttgtgc
     2281 cgctgatgag cctaacaaaa atactcagga acacttgaaa aagttaaaga gtgctcctaa
     2341 attagagaca cagagatcgc attccaccat tcaacctatg agcagtagtg gggcagagaa
     2401 ggttcaaggg gatttggcgc atgggaatat taacgatgcg gcatataagt ctaccacaga
     2461 tgaagctgct aagggggatg gtcaaaatgg aaatcagaca gttgcagaaa gcaacataaa
     2521 gggaactgat aatattgaaa atgaggctgc caaaaatgtt gatacgtata aattcgtaac
     2581 tgaaaggagt gctgacacta ggggcgctac agatattaca gaaacggggg aggagaaatt
     2641 gaacacatca tatagtggat cttctgagat taccgttaag gaaaacatcc caggtgatgg
     2701 tattgttaag gatgtatctg cagctgtaga aaatagtgaa aacccattgg aaacgaagca
     2761 taaaattttt gagccttcca aagataacag tgataacagt gaaaacagtg gatccatgga
     2821 atttaaggca accagttcga atccgataac tgaggctgta gaaagtagta gcgcggaggg
     2881 gcaggttcag gaggattctg cacacagaag tgttaacact ggcagggata actctacaat
     2941 aagtgcagct acttccgatg atggcctttc ctctggtgat aaacgtgttg aaagtcttac
     3001 ttccattgag aatgctgatg atggcggtga tcctgtgcaa ggttctttat ggaacctgct
     3061 gaatgatcca tcagttggtg ctggaggagg aaaatctcac attaaaacag aggagaatga
     3121 aggaagccag gctgaaatag atggaaaaaa tgtagacatt gccgaacaga gaactgcgac
     3181 catcactgaa gtgcagcctg agcgacctga tctttctgat actgataatg gtaatgttcc
     3241 tcgatctggc aataagcaaa atgagggtgc aactgcgttg agtggagctg aaagtttgga
     3301 atcaaacgaa agtgtacata aaactattga taatacaact cacggtttag aaaataaaaa
     3361 tggaggaaac gaaaaggatt ttcaaaagca tgattttatg aataatgaca tgttgaatga
     3421 tcaaacaagt tcagatcaaa caagttctga tcaaacaagt tcaaatcaaa caagttctga
     3481 tcaaacaagt tcaaatcaaa caagttctga tcaaacaagt tctgatcaaa taagttcaga
     3541 ccaaacaagt tcagatcaaa caagttcaaa tcaaacaagt tctgatcaaa ctatagatac
     3601 agaagaacat cacagggata atgtcagaaa tcctgaaata aagagttcgg aagatatgag
     3661 taaaggggat tttatgagaa attcaaatag taacgagtta tatagtcata ataatttgaa
     3721 taatcgtaaa ttaaatatag accaatacga acacagagat gtcaaagcaa caagggaaaa
     3781 aattatcctt atgtctgaag taaacaaatg caataatagg gcatccttaa aatactgtaa
     3841 cactatagaa gacagaatgt tatcgagcac ttgttcaagg gagaggagta aaaatttatg
     3901 ttgttcaata tcggattttt gtttgaatta ttttgagctc tatccttatg aattttataa
     3961 ttgcatgaaa aaggaatttg aagattcatc atacgagtgc tttacaaaag gaagctccac
     4021 aggtatacag gaaaagatga tcaatggaca aagatgttac gtacattgaa aataaattca
     4081 ttttaggaat gttataaatt tttttgataa tcaatatctc ctttgcaggc atagtttatt
     4141 ttgccacggg gggagcgttt ctgataatac tgctactatt tgtctcaaag aatgttgcct
     4201 cgaatgagta accaaaaaat aatataaaaa ttagaataaa aattagagtg gaagctagat
     4261 taacaattaa aataaaaaat aaaatagaaa gtgctgttaa tgcacaatta attctatata
     4321 ttccatgtgt gaaattttag ggagagtaaa aatgtataat cattatatgc atgcacgtat
     4381 acatatatag acatatatgt acctacatat aatgaatgca cacttcctag ttcgtacagt
     4441 tatgaagagg aagctacatt tgatgagttc gtggaatatt ctgatgatat tcacagaacc
     4501 cctctaatgc ctaaccatat tgagcacatg caacaattta cacccctgga ttattcataa
     4561 tgctactact tgggtaagta aagaaagttg tgattgtaca tgctgatgga atttttgtca
     4621 ttttgccttt gcagtaaatg agaaagtata tgaagctttc gcaagaatga tttaacagca
     4681 ctatgacaat tacttgtact attgcgctta ttaaacatct aaattagaat taatttttgc
     4741 atttgtaata agttcaatac aa
//