From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: B.subtilis RNase PH (rph) gene, complete cds.
Date: 31 Jul 92 10:00:27 GMT
Distribution: bionet
Checksum: 51802     4

LOCUS       BACRPHA      2067 bp ds-DNA             BCT       31-JUL-1992
DEFINITION  B.subtilis RNase PH (rph) gene, complete cds.
ACCESSION   M85163
KEYWORDS    RNase PH.
SOURCE      Bacillus subtilis DNA.
  ORGANISM  Bacillus subtilis
            Prokaryotae; Firmicutes; Endospore-forming rods and cocci;
            Bacillaceae.
REFERENCE   1  (bases 1 to 2067)
  AUTHORS   Craven,M.G., Henner,D.J., Alessi,D., Schauer,A.T., Ost,K.A.,
            Deutscher,M.P. and Friedman,D.I.
  TITLE     Identification of the rph (RNase PH) gene of Bacillus subtilis:
            Evidence for suppression of cold-sensitive mutations in Escherichia
            coli
  JOURNAL   J. Bacteriol. 174, 4727-4735 (1992)
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     CDS             1258..1995
                     /product="RNase PH"
                     /gene="rph"
                     /codon_start=1
                     /translation="MRHDGRQHDELRPITFDLDFISHPEGSVLITAGNTKVICNASVE
                     DRVPPFLRGGGKGWITAEYSMLPRATNQRTIRESSKGKISGRTMEIQRLIGRALRAVV
                     DLEKLGERTIWIDCDVIQADGGTRTASITGAFLAMAIAIGKLIKAGTIKTNPITDFLA
                     AISVGIDKEQGILLDLNYEEDSSAEVDMNVIMTGSGRFVELQGTGEEATFSREDLNGL
                     LGLAEKGIQELIDKQKEVLGDSLPELK"
BASE COUNT      650 a    404 c    531 g    482 t
ORIGIN
        1 ggtctatttc ctaaagaggc tcgtatacat aatagtacaa catccttagg agggaaagta
       61 tgctgaaaaa aggacctgca gtcattggtg ccacttgtct cacctctgcc ttgcttttat
      121 ccggatgcgg tttgtttcaa tctgataagg cagccgagga aattgatccg cctcaagacg
      181 ttacattcgt gaacgatgaa gcgggtgcca attcaaatac aacagcagcc aaaaaaacag
      241 aaagtgaaaa aagcgatacc gcaaaagcga tcaagcctca tctactgtta tgagggagct
      301 ttatctcatt gataaaaacg ggtatgttgt tgcgcaaacg ctgcctcttc ctaaaagtga
      361 gagcacggct aagcaggcgc ttgaatacct cgtacagggc gggcctgtgt ctgaaattct
      421 tccgaatggc ttcagagctg tattgccggc tgacacacgt taacgttgac atcaaaaaag
      481 atggaccggc gatagctgga tttctctaat gagttcaaaa attataagaa agaagacgaa
      541 cagaaatcgt tcaatctgtg acgtggacgc tgacgcaatt tagttcgata gataaagtga
      601 agctgagaat aaacgggcat gaactgaagg agatgcctgt aggagggacg ccgatctcag
      661 atgatttaag ccgcaaagac ggcattaatt tggaaacagc cggtgtaaat gatctcactg
      721 ctacccatcc gctgacagtc tactatttgg cggaaaatga agacagtgaa tactacgtgc
      781 cggtgaccaa acgaatcgac aattcggaaa aagacgcatc acagcagcca ttaatgaatt
      841 ggctaaaggg ccgagcaagg tgtgcgggct gttaacagac ttcagtgaag atgtaagctg
      901 gtcagcaacc gaatcaagac gggcgcgtga cgttggattc aatcaatcta taggcagcgc
      961 cgatgagaag acgaaaatga tttcttcaga agtgctgaac agcattgtgc tgacgttaac
     1021 ggagcagccg gatgtaaaaa gcgtctctgt taaagtgaac ggaaaatcag agctggtaaa
     1081 tgaaaaaggc gaaaagctca cagaaccggt ctccagacca tctcaagtga atacgggtag
     1141 tttttaaatt gggatttttg atatactagg aagaaagagg ttagcgttcc ggctgcctct
     1201 ttctttattc aattccggga caaaaatggt ttatgtacat acaatcggag gtaaaacatg
     1261 agacatgacg gaagacaaca cgatgagctg cgtccaatca cattcgattt ggattttatc
     1321 agccatccgg aaggttctgt tctaataaca gcgggaaata caaaggtaat ctgcaacgcg
     1381 tctgttgagg accgcgtacc gccgtttttg cggggcggag ggaaaggctg gattacagca
     1441 gagtacagca tgctgccgcg ggcaacaaac caaagaacaa tcagagaatc ttctaagggg
     1501 aaaatttccg gcagaacaat ggagatccaa cgcctgatcg gccgcgcgct tcgagctgtt
     1561 gtagatctgg aaaagctcgg tgaacggacg atttggattg actgtgatgt cattcaagct
     1621 gatggaggaa ctagaacagc ttccattaca ggcgcatttc ttgctatggc gattgcgatt
     1681 ggcaagctga tcaaagcagg aacaatcaag acaaatccga tcactgattt tcttgctgca
     1741 atctctgttg gaattgataa ggaacaggga attttgttag atttaaatta tgaagaagat
     1801 tcctctgctg aagttgacat gaatgtcatc atgacaggca gcggccgttt tgtggaattg
     1861 cagggaaccg gggaagaggc aactttttct agagaggatc tgaacggtct tctgggtctt
     1921 gcggaaaaag gaatccaaga gctgattgac aagcaaaaag aagtgctggg tgattctctg
     1981 ccagaactga aataaagaca gaaaggcttg atcatcatga aagaagcaat tattgcaaca
     2041 cataatccgg gaaaagtgaa ggaattc
//