From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: B.circulans gene for cyclodextrin glycosyltransferase
Date: 28 Dec 92 21:25:47 GMT
Distribution: bionet
Checksum: 49393 6

LOCUS       BACCGTASE    2383 bp ds-DNA             BCT       01-DEC-1992
DEFINITION  B.circulans gene for cyclodextrin glycosyltransferase
ACCESSION   X68326
KEYWORDS    cyclodextrin synthase; cyclodextrin-glycosyltransferase.
SOURCE      Bacillus circulans DNA.
  ORGANISM  Bacillus circulans
            Prokaryotae; Firmicutes; Endospore-forming rods and cocci;
            Bacillaceae.
REFERENCE   1  (bases 1 to 2383)
  AUTHORS   Nitschke,L., Heeger,K., Bender,H. and Schulz,G.E.
  TITLE     Molecular cloning, nucleotide sequence and expression in
            Escherichia coli of the beta-cyclodextrin glycosyltransferase gene
            from Bacillus circulans strain no. 8
  JOURNAL   Appl. Microbiol. Biotechnol. 33, 542-546 (1990)
  STANDARD  full automatic
COMMENT     *source: clone_library=lambda EMBL3; *source: clone=pBC2;
               From EMBL    entry BCCGTASE;  dated 05-OCT-1992.
FEATURES             Location/Qualifiers
     mat_peptide     324..2375
                     /product="cyclodextringlycosyltransferase"
                     /gene="CGTase"
     CDS             222..2378
                     /gene="CGTase"
                     /product="cyclodextringlycosyltransferase"
                     /codon_start=1
                     /translation="MFQMAKRAFLSTTLTLGLLAGSALPFLPASAVYADPDTAVTNKQ
                     SFSTDVIYQVFTDRFLDGNPSNNPTGAAYDATCSNLKLYCGGDWQGLINKINDNYFSD
                     LGVTALWISQPVENIFATINYSGVTNTAYHGYWARDFKKTNPYFGTMADFQNLITTAH
                     AKGIKIVIDFAPNHTSPAMETDTSFAENGRLYDNGTLVGGYTNDTNGYFHHNGGSDFS
                     SLENGIYKNLYDLADFNHNNATIDKYFKDAIKLWLDMGVDGIRVDAVKHMPLGWQKSW
                     MSSIYAHKPVFTFGEWFLGSAASDADNTDFANKSGMSLLDFRFNSAVRNVFRDNTSNM
                     YALDSMINSTATDYNQVNDQVTFIDNHDMDRFKTSAVNNRRLEQALAFTLTSRGVPAI
                     YYGTEQYLTGNGDPDNRAKMPSFSKSTTAFNVISKLAPLRKSNPAIAYGSTQQRWINN
                     DVYVYERKFGKSVAVVAVNRNLSTSASITGLSTSLPTGSYTDVLGGVLNGNNITSTNG
                     SINNFTLAAGATAVWQYTTAETTPTIGHVGPVMGKPGNVVTIDGRGFGSTKGTVYFGT
                     TAVTGAAITSWEDTQIKVTIPSVAAGNYAVKVAASGVNSNAYNNFTILTGDQVTVRFV
                     VNNASTTLGQNLYLTGNVAELGNWSTGSTAIGPAFNQVIHQYPTWYYDVSVPAGKQLE
                     FKFFKKNGSTITWESGSNHTFTTPASGTATVTVNWQ"
BASE COUNT      632 a    633 c    559 g    559 t
ORIGIN      
        1 tttgttgacg tctttccggt gcatttgtcc tgattctggt atcgtgctgt tatcaatggc
       61 aaagaagtat ggaggtgggg gcgctaatca atcgagagcc tctgctgact gtacactctc
      121 gcatcactag cagacgggtt aagatgagcc agtcgccttc cgaatccggc accacaaatg
      181 ccgcacgaac attgtactac attcacgaag ggtggattac catgtttcaa atggccaaac
      241 gcgcattcct cagcaccaca ctgaccctcg gcttgcttgc cggcagcgcc ctgccgttct
      301 tgccagcttc cgctgtatac gccgatccgg acactgctgt gaccaacaaa cagagcttca
      361 gtacagatgt gatctaccaa gtatttacgg atcgcttttt ggacggcaat ccctccaaca
      421 accccaccgg agccgcttat gatgccacat gcagcaattt gaagctgtac tgcggcggag
      481 actggcaagg gttaatcaac aaaatcaacg ataactattt cagtgatctg ggtgtcacag
      541 cgttgtggat ctcccagcct gtcgaaaata tttttgcgac gatcaactat agcggtgtaa
      601 ccaatactgc ctaccacggc tactgggctc gagacttcaa aaagaccaat ccgtacttcg
      661 gcacgatggc ggactttcag aacctgatta cgacggctca tgccaaaggc atcaagattg
      721 tcattgactt tgcaccgaat cacacctctc cagcgatgga aaccgacacc tcttttgccg
      781 aaaatggcag actgtacgat aacggtacac tggtaggcgg ttataccaat gataccaacg
      841 ggtacttcca tcacaatggc ggctccgact tctcttccct ggagaacggc atctacaaaa
      901 acctgtatga cctggccgac ttcaaccaca ataatgcgac catcgacaaa tacttcaaag
      961 atgcgatcaa actgtggctt gatatgggcg ttgacggtat tcgggtggat gcggtgaaac
     1021 atatgcctct cggttggcaa aagagctgga tgtcctccat ctacgcacac aaacccgtgt
     1081 tcacttttgg agaatggttc ttgggatcag ctgcatccga tgcagataac acggattttg
     1141 ctaacaagtc tggtatgagc ctgctcgact tccgctttaa ctctgcggtg cgtaatgtgt
     1201 tccgtgacaa cacgtccaac atgtacgctc tggattccat gattaacagc acagctacgg
     1261 actacaacca agtgaacgat caggtgacgt tcatcgacaa ccatgatatg gatcgtttca
     1321 aaacaagtgc ggtcaacaat cgccgtctgg aacaggcttt ggccttcaca ttgacttcac
     1381 gtggtgtacc tgccatctac tatggtactg aacagtattt gacggggaat ggcgatccag
     1441 ataaccgggc caaaatgcct tcgttctcca aatccactac agcttttaac gtcataagta
     1501 aactcgcgcc tctgcgcaaa tccaatccgg ccattgccta cggttccaca cagcagcgct
     1561 ggattaataa tgatgtatat gtttatgagc gtaaattcgg caagagcgta gccgttgtcg
     1621 cggtaaaccg caatctttcc acgtctgcaa gcattacagg gttaagcact tccctgccta
     1681 caggctcata cacggatgtg cttggcgggg tgctgaacgg aaataacatc acctccacga
     1741 atggtagcat taacaacttc acccttgctg cgggtgcaac ggcagtatgg caatacacaa
     1801 ctgccgaaac aacaccaacc atcggtcatg ttggtccggt tatggggaaa cccggtaatg
     1861 tggtgacaat tgatggccgt ggattcggct cgacgaaagg cacggtctac ttcggcacta
     1921 cagcggttac cggagcagcg attacgtctt gggaagatac acagattaaa gtaaccatcc
     1981 cttccgttgc cgctggcaac tatgcagtca aagttgcggc aagcggggta aacagcaatg
     2041 catacaacaa cttcaccatc ctgactggcg atcaggtcac cgttcgcttc gtcgtaaaca
     2101 atgcgtccac aacgcttgga cagaacctct atttgacagg caacgtagcc gagcttggca
     2161 actggagcac cggttcgact gccattggac ccgcattcaa tcaggtcatt catcaatacc
     2221 caacctggta ctatgatgtc agcgtaccgg caggcaaaca gctggagttc aaatttttca
     2281 agaaaaacgg ttcaacgatt acatgggaaa gcggttctaa ccacacattc actacaccag
     2341 cgagcggaac agccaccgtt acggtgaact ggcagtaaag ctt
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