HDTenEightyP/NetNews huggingface dataset:
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human chromosome 4 sequence-tagged site STS4-86
Date: 21 Aug 92 10:11:15 GMT
Distribution: bionet
Checksum: 44494     3

LOCUS       HUM4STS86     398 bp ds-DNA             PRI       21-AUG-1992
DEFINITION  Human chromosome 4 sequence-tagged site STS4-86
ACCESSION   M95212
KEYWORDS    STS; human chromosome 4; sequence tagged site.
SOURCE      Homo sapiens, clone C4-1 from Los Alamos National Laboratory
            chromosome 4 cosmid library, plate 4-1-176R, pos 4A; vector
            SuperCos 1 (Stratagene)
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 398)
  AUTHORS   Goold,R.D., diSibio,G., Dugaiczyk,A., Smith,K.A., Xu,H.,
            Magrane,G., Lang,D.B., Cox,D.R., Masters,S.B. and Myers,R.M.
  TITLE     Sequence-Tagged Sites for Human Chromosome 4
  JOURNAL   Unpublished (1992) see COMMENT for author address
  STANDARD  full automatic
COMMENT     PCR components: 25 ng of human genomic DNA, 10 pmol of each
            oligonucleotide, 200 micro-M dNTPs, 0.25 U Taq  polymerase (Cetus)
            in 10 micro-l of 50 mM KCl-20 mM  Tris-HCl, pH 8.3 (at room temp),
            2.5 mM MgCl-2.  Initial denaturation at 94degC for 1.5 min, then 30
            cycles of  94degC for 15 sec, 62degC for 23 sec, and 72degC for 30
            sec, followed by a final extension at 72degC for 3.5 min, using a
            Perkin-Elmer 9600 thermocycler. PCR-amplified product size 222 bp.
            Sequence submitted by:
            Human Genome Mapping Center
            Box 0925
            University of California San Francisco
            San Francisco, CA 94143-0925  USA
            Phone: (415) 502-1612   Fax: (415) 476-8391
            e-mail: hgmcprobes@cgl.ucsf.edu
FEATURES             Location/Qualifiers
     misc_feature    55..78
                     /label=Primer1
     misc_feature    complement(255..276)
                     /label=Primer2
BASE COUNT      108 a     89 c     66 g    120 t     15 others
ORIGIN
        1 taccccnctc ttnggnggtt ncatggttta ctctggactc tttctgatgt aaatcaattc
       61 cataaccttc tgtacctctc agacttctct ctcattccta ctgcttttag natcacaaaa
      121 gactaagaat tgagtagatt cacagacgtg ttcttggaaa ggagggacgt tttaaagagg
      181 aaaaatgcat ttattncaaa tttaatattt ttctttattt actgnccaaa cccagtcaaa
      241 tttctttccc gntcattccc ttctctccat tcctggntgt aacaattaca gcccagtgtc
      301 aaggactgtg aaaggactga aattnccctc ctaactgtaa gtgaacaaga naaactntca
      361 cagttccang gatgctggnn gaagactcaa gacccggg
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human chromosome 4 sequence-tagged site STS4-426
Date: 21 Aug 92 10:10:12 GMT
Distribution: bionet
Checksum: 36632     3

LOCUS       HUM4STS426    434 bp ds-DNA             PRI       21-AUG-1992
DEFINITION  Human chromosome 4 sequence-tagged site STS4-426
ACCESSION   L00852
KEYWORDS    STS; human chromosome 4; sequence tagged site.
SOURCE      Homo sapiens, clone p4-444 from a chromosome 4 plasmid library;
            vector pBluescript II KS + (Stratagene)
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 434)
  AUTHORS   Goold,R.D., diSibio,G., Dugaiczyk,A., Smith,K.A., Xu,H.,
            Magrane,G., Lang,D.B., Cox,D.R., Masters,S.B. and Myers,R.M.
  TITLE     Sequence-Tagged Sites for Human Chromosome 4
  JOURNAL   Unpublished (1992) see COMMENT for author address
  STANDARD  full automatic
COMMENT     PCR components: 25 ng of human genomic DNA, 10 pmol of each
            oligonucleotide, 200 micro-M dNTPs, 0.25 U Taq  polymerase (Cetus)
            in 10 micro-l of 50 mM KCl-20 mM  Tris-HCl, pH 8.3 (at room temp),
            2.5 mM MgCl-2.  Initial denaturation at 94degC for 1.5 min, then 30
            cycles of  94degC for 15 sec, 62degC for 23 sec, and 72degC for 30
            sec, followed by a final extension at 72degC for 3.5 min, using a
            Perkin-Elmer 9600 thermocycler. PCR-amplified product size 267 bp.
            Sequence submitted by:
            Human Genome Mapping Center
            Box 0925
            University of California San Francisco
            San Francisco, CA 94143-0925  USA
            Phone: (415) 502-1612   Fax: (415) 476-8391
            e-mail: hgmcprobes@cgl.ucsf.edu
FEATURES             Location/Qualifiers
     misc_feature    26..50
                     /label=Primer1
     misc_feature    complement(267..292)
                     /label=Primer2
BASE COUNT      150 a     67 c     58 g    150 t      9 others
ORIGIN
        1 gatcaataaa tgnaaacaaa aatgtatcct ggcaattctt aagacatttc taatattact
       61 ttaccaataa tttaaagcta gcttatttat tcaatatttt acttgttatg taaactagaa
      121 aaagcatttg actagtcttt tctntttccc tgatgaggta tttgattcaa gcacttttat
      181 tttcttaagc caatgaatta gagctctttt atatatttnc agnagtgaaa tattgtgtac
      241 agaacacata aatacataga tgtattaggt atgttgatag aagtacatct tatggattca
      301 caaagaccnn cttgtttttc cttagacttt caaattcttg ataacatgct tcattaccct
      361 aggcaggtgt cagctaaata acctaaactn gcatattaan ggaancaact caggtgaaaa
      421 tcaaatagca aaac
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Collybia earleae ribosomal RNA (44LR6) large subunit, partial.
Date: 18 Aug 92 10:00:31 GMT
Distribution: bionet
Checksum: 17180     2

LOCUS       COQRRLSA      249 bp ss-rRNA            RNA       18-AUG-1992
DEFINITION  Collybia earleae ribosomal RNA (44LR6) large subunit, partial.
ACCESSION   M98632
KEYWORDS    large subunit ribosomal RNA.
SOURCE      Collybia earleae (library: Duke culture collection 50) cDNA to
            rRNA.
  ORGANISM  Collybia earleae
            Eukaryota; Fungi; Amastigomycota; Basidiomycetes.
REFERENCE   1  (bases 1 to 249)
  AUTHORS   Hibbett,D.S. and Vilgalys,R.
  TITLE     Phylogenetic relationships of the basidiomycete genus Lentinus
            inferred from molecular and morphological characters
  JOURNAL   Unpublished (1992)
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     rRNA            <1..>249
                     /note="Approximately aligns to region between positions
                     845 to 1093in Saccharomyces cerevisiae large subunit
                     nuclear rRNA."
                     /product="large subunit ribosomal RNA"
                     /citation=[1]
BASE COUNT       69 a     50 c     59 g     71 t
ORIGIN
        1 gaaactctgg tggaggctcg tagcgattct gacgtgcaaa tcgatcgtcg aatttgggta
       61 taggggcgaa agactaatcg aaccatctag tagctggttc ctgccgaagt ttccctcaga
      121 tagcagaaac tcattgtcag atttatgtgg taaagcgaat gattagaggc cttggggttc
      181 gtaacaacct taacctattc tcaaacttta aatatgtaag aacgagctgt ctcttaattg
      241 gctctactc
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human chromosome 4 sequence-tagged site STS4-327
Date: 21 Aug 92 10:08:58 GMT
Distribution: bionet
Checksum: 32880     3

LOCUS       HUM4STS327    481 bp ds-DNA             PRI       21-AUG-1992
DEFINITION  Human chromosome 4 sequence-tagged site STS4-327
ACCESSION   L00797
KEYWORDS    STS; human chromosome 4; sequence tagged site.
SOURCE      Homo sapiens, clone p4-272 from a chromosome 4 plasmid library;
            vector pBluescript II KS + (Stratagene)
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 481)
  AUTHORS   Goold,R.D., diSibio,G., Dugaiczyk,A., Smith,K.A., Xu,H.,
            Magrane,G., Lang,D.B., Cox,D.R., Masters,S.B. and Myers,R.M.
  TITLE     Sequence-Tagged Sites for Human Chromosome 4
  JOURNAL   Unpublished (1992) see COMMENT for author address
  STANDARD  full automatic
COMMENT     PCR components: 25 ng of human genomic DNA, 10 pmol of each
            oligonucleotide, 200 micro-M dNTPs, 0.25 U Taq  polymerase (Cetus)
            in 10 micro-l of 50 mM KCl-20 mM  Tris-HCl, pH 8.3 (at room temp),
            2.5 mM MgCl-2.  Initial denaturation at 94degC for 1.5 min, then 30
            cycles of  94degC for 15 sec, 64degC for 23 sec, and 72degC for 30
            sec, followed by a final extension at 72degC for 3.5 min, using a
            Perkin-Elmer 9600 thermocycler. PCR-amplified product size 213 bp.
            STS sequence contains a simple sequence tandem repeat (CA).
            Sequence submitted by:
            Human Genome Mapping Center
            Box 0925
            University of California San Francisco
            San Francisco, CA 94143-0925  USA
            Phone: (415) 502-1612   Fax: (415) 476-8391
            e-mail: hgmcprobes@cgl.ucsf.edu
FEATURES             Location/Qualifiers
     misc_feature    30..54
                     /label=Primer1
     misc_feature    complement(220..242)
                     /label=Primer2
BASE COUNT      158 a     91 c     77 g    147 t      8 others
ORIGIN
        1 gatccgaaga tgttaaaggt tacaaccttg cagccatgag atgaataatt tctggagacc
       61 tatggtgtag catgaggaac tatagttaat aataaaaata ttcttgaaat tttctaagag
      121 agtatatttt atgtgtcctc accacacaca cacacacaca cacacacaca cacacacaca
      181 caantggtaa ctatatgagg tggtgaatat attaattagc ttgagtgtgg taagcacttc
      241 acattgttta tgtacatcaa acattacatt ttaccccttt aatatgtaca atttttatta
      301 gacaattata tgtcaataaa gctaaaaaaa ctataatatc catatgtatt ttctcaccng
      361 ccttataaaa tttcttgtta tataaattct gatccnctag ttctagagcg gccgccaccg
      421 nggnggaggt ccaattcgnc ctatagngag tcggatttac aattcactgg cgncggtttc
      481 a
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Homo sapiens DNA-binding protein (ZNF) gene, partial cds.
Date: 21 Aug 92 10:11:50 GMT
Distribution: bionet
Checksum: 59614     2

LOCUS       HUMZNFG       172 bp ds-DNA             PRI       21-AUG-1992
DEFINITION  Homo sapiens DNA-binding protein (ZNF) gene, partial cds.
ACCESSION   M88363
KEYWORDS    DNA binding protein; zinc finger protein.
SOURCE      Homo sapiens Placenta DNA.
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 172)
  AUTHORS   Bray,P.L., Lichter,P., Thiesen,H.J., Ward,D.C. and Dawid,I.B.
  TITLE     Characterization and mapping of human genes encoding zinc finger
            proteins
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 88, 9563-9567 (1991)
  STANDARD  full automatic
REFERENCE   2  (bases 1 to 172)
  AUTHORS   Lichter,P., Bray,P., Ried,T., Dawid,I.B. and Ward,D.C.
  TITLE     Clustering of C2-H2 zinc finger motif sequences within telomeric
            and fragile site regions of human chromosomes
  JOURNAL   Genomics 13, 999-1007 (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     CDS             1..172
                     /standard_name="zinc finger protein"
                     /product="DNA binding protein"
                     /gene="ZNF"
                     /note="putative"
                     /codon_start=1
                     /translation="IHTGEKPYECSNCRKAFSHKEKLIKHYKIHSREQSYKCNECGKA
                     FIKMSNLIRHQRI"
                     /partial
BASE COUNT       70 a     26 c     29 g     47 t
ORIGIN
        1 attcatactg gagagaagcc ctatgaatgt agtaactgta gaaaagcctt cagtcacaag
       61 gaaaaactta ttaaacatta taaaattcac agtagggagc agtcttacaa atgtaatgaa
      121 tgtggtaaag ctttcattaa aatgtcaaat ctcattagac atcaaagaat tc
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Cichlosoma citrinellum mitochondrion ribosomal RNA small subunit
Date: 21 Aug 92 10:06:05 GMT
Distribution: bionet
Checksum: 13333     2

LOCUS       CICMTRRNA     313 bp ds-DNA             ORG       21-AUG-1992
DEFINITION  Cichlosoma citrinellum mitochondrion ribosomal RNA small subunit
            gene, 3' end.
ACCESSION   M87533
KEYWORDS    ribosomal RNA; ribosomal RNA small subunit.
SOURCE      Mitochondrion Cichlosoma citrinellum (chromosome Mitochondrion
            Cichlosoma citrinellum) DNA.
  ORGANISM  Mitochondrion Cichlosoma citrinellum
            Eukaryota; Animalia; Chordata; Vertebrata; Osteichthyes;
            Actinopterygii; Perciformes; Percoidei; Cichlidae.
REFERENCE   1  (bases 1 to 313)
  AUTHORS   Meyer,A. and Dolven,S.I.
  TITLE     Molecules, fossils and the origin of tetrapods
  JOURNAL   J. Mol. Evol. 35, 102-113 (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     rRNA            <1..293
BASE COUNT      103 a     75 c     70 g     65 t
ORIGIN
        1 ggaactacga gcattagctt aaaacccaaa ggacttggcg gtgctttaga cccccctaga
       61 ggagcctgtt ctagaaccga taatccccgt taaacctcac cctcccttgt catccccgcc
      121 tatataccgc cgtcgtcagc ttaccctgtg aaggcactat agtaagcaaa actagtaaaa
      181 cccaaaacgc caggtcgagg tgtagcatat gagagggaaa gaaatgggct acattcccta
      241 ctttagggaa cacggataat gtaatgaaaa gtacattaga aggaggattt agcagtaagc
      301 aaaaatagag gca
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human gene for branched chain alpha-keto acid dehydrogenase (EC
Date: 20 Aug 92 10:02:09 GMT
Distribution: bionet
Checksum: 62309     3

LOCUS       HUMBCKD06     139 bp ds-DNA             PRI       20-AUG-1992
DEFINITION  Human gene for branched chain alpha-keto acid dehydrogenase (EC
            1.2.4.4) E-1-beta subunit, exon 6.
ACCESSION   D90387
KEYWORDS    branched chain alpha-keto acid dehydrogenase;
            maple syrup urine disease.
SEGMENT     6 of 10
SOURCE      Human adult peripheral blood, white blood cell DNA.
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 16 to 124)
  AUTHORS   Matsuda,I., Asaka,J., Akaboshi,I., Endo,F., Mitsubuchi,H. and
            Nobukuni,Y.
  TITLE     Maple syrup urine disease: Complete primary structure of the
            E-1-beta subunit of human branched chain alpha-ketoacid
            dehydrogenase complex deduced from the nucleotide sequence and a
            gene analysis of patients with this disease
  JOURNAL   J. Clin. Invest. 86, 242-247 (1990)
  STANDARD  simple staff_review
REFERENCE   2  (bases 1 to 139)
  AUTHORS   Mitsubuchi,H., Nobukuni,Y., Endo,F. and Matsuda,I.
  TITLE     Structural organization and chromosomal localization of the gene
            for the E-1-beta subunit of human branched chain alpha-keto acid
            dehydrogenase
  JOURNAL   Unpublished (1991)
  STANDARD  full staff_review
COMMENT     These data kindly submitted in computer readable form by:
            Hiroshi Mitsubuchi
            Department of Pediatrics
            Kumamoto University Medical School
            1-1-1 Honjo, Kumamoto 860
            Japan
            Phone:  096-344-2111 x5654
            Fax:    096-366-3471
FEATURES             Location/Qualifiers
     intron          <1..15
                     /note="intron 5"
     exon            16..124
                     /number=6
                     /note="E-1-beta subunit"
     intron          125..>139
                     /note="intron 6"
BASE COUNT       40 a     25 c     26 g     48 t
ORIGIN      Chromosome 6.
        1 aatctgtttt tgcaggtggt tatacccaga agccctttcc aggccaaagg acttcttttg
       61 tcatgcatag aggataaaaa tccttgtata ttttttgaac ctaaaatact ttacagggca
      121 gcaggtaaag attttcttt
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Homo sapiens alpha-1,3 fucosyltransferase 6 (FCT3A) mRNA, complete
Date: 15 Aug 92 10:02:07 GMT
Distribution: bionet
Checksum: 45245     3

LOCUS       HUMFCT3A     1126 bp ss-mRNA            PRI       15-AUG-1992
DEFINITION  Homo sapiens alpha-1,3 fucosyltransferase 6 (FCT3A) mRNA, complete
            cds.
ACCESSION   M98825
KEYWORDS    alpha-1,3 fucosyltransferase 6.
SOURCE      Homo sapiens cDNA to mRNA.
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 1126)
  AUTHORS   Koszdin,K.L. and Bowen,B.R.
  TITLE     The cloning and expression of a human alpha-1,3 fucosyltransferase
            capable of forming the E-selectin ligand
  JOURNAL   Biochem. Biophys. Res. Commun. (1992) In press
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     CDS             16..1095
                     /product="alpha-1,3 fucosyltransferase 6"
                     /gene="FCT3A"
                     /codon_start=1
                     /translation="MDPLGPAKPQWSWRCCLTTLLFQLLMAVCFFSYLRVSQDDPTVY
                     PNGSRFPDSTGTPAHSIPLILLWTWPFNKPIALPRCSEMVPGTADCNITADRKVYPQA
                     DAVIVHHREVMYNPSAQLPRSPRRQGQRWIWFSMESPSHCWQLKAMDGYFNLTMSYRS
                     DSDIFTPYGWLEPWSGQPAHPPLNLSAKTELVAWAVSNWGPNSARVRYYQSLQAHLKV
                     DVYGRSHKPLPQGTMMETLSRYKFYLAFENSLHPDYITEKLWRNALEAWAVPVVLGPS
                     RSNYERFLPPDAFIHVDDFQSPKDLARYLQELDKDHARYLSYFRWRETLRPRSFSWAL
                     AFCKACWKLQEESRYQTRGIAAWFT"
BASE COUNT      211 a    391 c    321 g    203 t
ORIGIN
        1 cagatactct gacccatgga tcccctgggc ccggccaagc cacagtggtc gtggcgctgc
       61 tgtctgacca cgctgctgtt tcagctgctg atggctgtgt gtttcttctc ctatctgcgt
      121 gtgtctcaag acgatcccac tgtgtaccct aatgggtccc gcttcccaga cagcacaggg
      181 acccccgccc actccatccc cctgatcctg ctgtggacgt ggccttttaa caaacccata
      241 gctctgcccc gctgctcaga gatggtgcct ggcacggctg actgcaacat cactgccgac
      301 cgcaaggtgt atccacaggc agacgcggtc atcgtgcacc accgagaggt catgtacaac
      361 cccagtgccc agctcccacg ctccccgagg cggcaggggc agcgatggat ctggttcagc
      421 atggagtccc caagccactg ctggcagctg aaagccatgg acggatactt caatctcacc
      481 atgtcctacc gcagcgactc cgacatcttc acgccctacg gctggctgga gccgtggtcc
      541 ggccagcctg cccacccacc gctcaacctc tcggccaaga ccgagctggt ggcctgggca
      601 gtgtccaact gggggccaaa ctccgccagg gtgcgctact accagagcct gcaggcccat
      661 ctcaaggtgg acgtgtacgg acgctcccac aagcccctgc cccagggaac catgatggag
      721 acgctgtccc ggtacaagtt ctatctggcc ttcgagaact ccttgcaccc cgactacatc
      781 accgagaagc tgtggaggaa cgccctggag gcctgggccg tgcccgtggt gctgggcccc
      841 agcagaagca actacgagag gttcctgccg cccgacgcct tcatccacgt ggacgacttc
      901 cagagcccca aggacctggc ccggtacctg caggagctgg acaaggacca cgcccgctac
      961 ctgagctact ttcgctggcg ggagacgctg cggcctcgct ccttcagctg ggcactcgct
     1021 ttctgcaagg cctgctggaa actgcaggag gaatccaggt accagacacg cggcatagcg
     1081 gcttggttca cctgagaggc ccggcatggg gcctgggctg ccaggg
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Rattus norvegicus polymorphic microsatellite repetitive element.
Date: 14 Aug 92 10:01:19 GMT
Distribution: bionet
Checksum: 21344     2

LOCUS       RATMCRSAT     397 bp ds-DNA             ROD       14-AUG-1992
DEFINITION  Rattus norvegicus polymorphic microsatellite repetitive element.
ACCESSION   M98521
KEYWORDS    polymorphic microsatellite.
SOURCE      Rattus norvegicus (strain LEW/N) liver DNA.
  ORGANISM  Rattus norvegicus
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Rodentia; Myomorpha; Muridae; Murinae.
REFERENCE   1  (bases 1 to 397)
  AUTHORS   Zha,H., Remmers,E.F., Goldmuntz,E.A., Cash,J.M., Crofford,L.J.,
            Mathern,P. and Wilder,R.L.
  TITLE     Linkage map of 10 polymorphic markers on rat chromosome 2
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
REFERENCE   2  (sites)
  AUTHORS   Zha,H., Wilder,R.L., Goldmuntz,E.A., Cash,J.M., Crofford,L.J.,
            Mathern,P. and Remmers,E.F.
  TITLE     Linkage map of 10 polymorphic markers on rat chromosome 2
  JOURNAL   Unpublished (1992)
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     satellite       68..119
                     /rpt_family="(dC-dA)n.(dG-dT)n"
                     /rpt_type=tandem
                     /note="microsatellite"
                     /citation=[2]
BASE COUNT       86 a     68 c    132 g    111 t
ORIGIN
        1 gatctagcct aggacccagg ggaaaagagg gagctgtagt tgttcccgga gtccttggct
       61 gggcagggtg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg
      121 agcttggtgg cagctgccac ctggagcgca tggaggtctt ctgcaggaga ttaggtggca
      181 gcccagtagg tgaagagctt ctctatgact ccccatgggg ggtcccatga aaagaggtag
      241 ctcatggttt caagacattt attttaatgg cggaaaatgg atgagtaaaa ctgtacccca
      301 cctgagatta agtacctttt gcaaggagga gtgtctggga agggaaattc attggctaaa
      361 cctctaggcc tttaggtacc tctttataat ggagatc
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human chromosome 4 sequence-tagged site STS4-102
Date: 21 Aug 92 10:06:30 GMT
Distribution: bionet
Checksum: 50654     3

LOCUS       HUM4STS102    416 bp ds-DNA             PRI       21-AUG-1992
DEFINITION  Human chromosome 4 sequence-tagged site STS4-102
ACCESSION   M95215
KEYWORDS    STS; human chromosome 4; sequence tagged site.
SOURCE      Homo sapiens, clone C4-6 from Los Alamos National Laboratory
            chromosome 4 cosmid library, plate 4-1-176R, pos 5B; vector
            SuperCos 1 (Stratagene)
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 416)
  AUTHORS   Goold,R.D., diSibio,G., Dugaiczyk,A., Smith,K.A., Xu,H.,
            Magrane,G., Lang,D.B., Cox,D.R., Masters,S.B. and Myers,R.M.
  TITLE     Sequence-Tagged Sites for Human Chromosome 4
  JOURNAL   Unpublished (1992) see COMMENT for author address
  STANDARD  full automatic
COMMENT     PCR components: 25 ng of human genomic DNA, 10 pmol of each
            oligonucleotide, 200 micro-M dNTPs, 0.25 U Taq  polymerase (Cetus)
            in 10 micro-l of 50 mM KCl-20 mM  Tris-HCl, pH 8.3 (at room temp),
            2.5 mM MgCl-2.  Initial denaturation at 94degC for 1.5 min, then 30
            cycles of  94degC for 15 sec, 63degC for 23 sec, and 72degC for 30
            sec, followed by a final extension at 72degC for 3.5 min, using a
            Perkin-Elmer 9600 thermocycler. PCR-amplified product size 183 bp.
            Sequence submitted by:
            Human Genome Mapping Center
            Box 0925
            University of California San Francisco
            San Francisco, CA 94143-0925  USA
            Phone: (415) 502-1612   Fax: (415) 476-8391
            e-mail: hgmcprobes@cgl.ucsf.edu
FEATURES             Location/Qualifiers
     misc_feature    29..53
                     /label=Primer1
     misc_feature    complement(186..210)
                     /label=Primer2
BASE COUNT      130 a     78 c     73 g     96 t     39 others
ORIGIN
        1 ncgagacatt ggtccactca ttncatngaa gcattattca caagaaccaa aaggtgaaaa
       61 cagtccagtc tattgatgaa tggaaggata ancaaaatat ggtatataca tacaatggaa
      121 tattattcaa acttaanaag gaagcaaatt ctgacatatg ctgcaacatg ggcgaaactt
      181 anaaaagtat gccaaaatga aatgtcacca nacgacaaat attttataat ttcatgtatc
      241 caaggtacct agtntngtca nnttactnca gatngngaac agcaagagtn nccagctgct
      301 gngggaanng gtaaatangg agttgtnctc ncantnggca tagtgtttca gncccngnct
      361 gntncnncag acgccnnnna tntttngccg gataccactc ccgtctctnt cccacn
//