From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: E.coli DNA polymerase (polB) gene, 5' flank.
Date: 30 Jul 92 10:01:12 GMT
Distribution: bionet
Checksum: 06696     9

LOCUS       ECOPOLBDA    4666 bp ds-DNA             BCT       30-JUL-1992
DEFINITION  E.coli DNA polymerase (polB) gene, 5' flank.
ACCESSION   M35371
KEYWORDS    DNA polymerase; polB gene.
SOURCE      E.coli (strain W3110) DNA.
  ORGANISM  Escherichia coli
            Prokaryotae; Gram-negative facultatively anaerobic rods;
            Enterobacteriaceae.
REFERENCE   1  (bases 1 to 4666)
  AUTHORS   Chen,H., Sun,Y., Stark,T., Beattie,W. and Moses,R.
  TITLE     Nucleotide sequence and deletion analysis of the polB gene of
            E.coli
  JOURNAL   DNA Cell Biol. 9, 613-635 (1990)
  STANDARD  simple staff_review
REFERENCE   2  (bases 1 to 4666)
  AUTHORS   Chen,H., Lawrence,C.B., Bryan,S.K. and Moses,R.E.
  TITLE     Aphidicolin inhibits DNA polymerase II of Escherichia coli, an
            alpha-like DNA polymerase
  JOURNAL   Nucleic Acids Res. 18, 7185-7186 (1990)
  STANDARD  simple staff_review
COMMENT     Draft entry and computer-readable sequence for [Unpublished (1990)]
            kindly submitted
            by H.Chen, 20-JUN-1990.
              Author address: H.Chen
              Baylor College of Medicine
              Cell Biology and IMG
              One Baylor Plaza
              Houston, TX 77030
              email: hchen@vulture.bcm.tmc.edu
FEATURES             Location/Qualifiers
     misc_feature    1193..1208
                     /note="lexA box"
     -35_signal      1209..1214
     -10_signal      1229..1234
     RBS             1250..1255
                     /note="ribosome binding site"
     CDS             1264..3570
                     /note="DNA polymerase (polB) (gtg start codon)"
                     /codon_start=1
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IPADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEACGQRIVYMLGPENGDASSLDFELEYVASRPQLLEKLNAWFANYDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRETIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTTGPEPP
                     GLPTFTTGLRTLSDPPATTRGGGNTPFY"
BASE COUNT     1100 a   1278 c   1257 g   1031 t
ORIGIN
        1 aagcttgcat gcctgcaggt cgactctaga ggatcctcgc tggtggcgcg caccataccg
       61 tcttcagcca tgcactgaac ctcaacgata tgcgccaatt cgccgagatg cacgacattg
      121 aaatcacggt gattgataac gacacacgcc tgccagcgtt taaagacgcg ctgcgctgga
      181 acgaagtgta ttacgggttt cgtcgctaag tagccgcatc cggtatgtaa cgcctgatgc
      241 gacgctgacg cgtcttatct ggcctacacg ctgcgatttt gtaggccgga taagcaaagc
      301 gcatccggca ttcaacgcct gatgcgacgc tggcgcgtct tatcaggcct acgcgctgcg
      361 attttgtagg ccggataagc aaagcgcatc cggcattcaa cgcctgatgc gacgctggcg
      421 cgtcttatca ggcctacacg ctgcgatttt gtaggccgga taagcaaagc gcatccggca
      481 cgaaggagtc aacatgttag aagatctcaa acgccaggta ttagaagcca acctggcgct
      541 gccaaaacac aacctggtca cgctcacatg gggcaacgtc agcgccgttg atcgcgagcg
      601 cggcgtcttt gtgatcaaac cttccggcgt cgattacagc gtcatgaccg ctgacgatat
      661 ggtcgtggtt agcatcgaaa ccggtgaagt ggttgaaggt acgaaaaagc cctcctccga
      721 cacgccaact caccggctgc tctatcaggc attcccctcc attggcggca ttgtgcatac
      781 gcactcgcgc cacgccacca tctgggcgca ggcgggtcag tcgattccag caaccggcac
      841 cacccacgcc gactatttct acggcaccat tccctgcacc cgcaaaatga ccgacgcaga
      901 aatcaacggc gaatatgagt gggaaaccgg taacgtcatc gtagaaacct ttgaaaaaca
      961 gggtatcgat gcagcgcaaa tgcccggcgt tctggtccat tcccacggcc cgtttgcatg
     1021 gggcaaaaat gccgaagatg cggtgcataa cgccatcgtg ctggaagagg tcgcttatat
     1081 ggggatattc tgccgtcagt tagcgccgca gttaccggat atgcagcaaa cgctgctgga
     1141 taaacactat ctgcgtaagc atggcgcgaa ggcatattac gggcagtaat gactgtataa
     1201 aaccacagcc aatcaaacga aaccaggcta tactcaagcc tggttttttg atggattttc
     1261 agcgtggcgc aggcaggttt tatcttaacc cgacactggc gggacacccc gcaagggaca
     1321 gaagtctcct tctggctggc gacggacaac gggccgttgc aggttacgct tgcaccgcaa
     1381 gagtccgtgg cgtttattcc cgccgatcag gttccccgcg ctcagcatat tttgcagggt
     1441 gaacaaggct ttcgcctgac accgctggcg ttaaaggatt ttcaccgcca gccggtgtat
     1501 ggcctttact gtcgcgccca tcgccaattg atgaattacg aaaagcgcct gcgtgaaggt
     1561 ggcgttaccg tctacgaggc cgatgtgcgt ccgccagaac gctatctgat ggagcggttt
     1621 atcacctcac cggtgtgggt cgagggtgat atgcacaatg gcactatcgt taatgcccgt
     1681 ctgaaaccgc atcccgacta tcgtccgccg ctcaagtggg tttctataga tattgaaacc
     1741 acccgccacg gtgagctgta ctgcatcggc ctggaagcgt gcgggcagcg catcgtttat
     1801 atgctggggc cggagaatgg cgacgcctcc tcgcttgatt tcgaactgga atacgtcgcc
     1861 agccgcccgc agttgctgga aaaactcaac gcctggtttg ccaactacga tcctgatgtg
     1921 atcatcggtt ggaacgtggt gcagttcgat ctgcgaatgc tgcaaaaaca tgccgagcgt
     1981 taccgtcttc cgctgcgtct tgggcgcgat aatagcgagc tggagtggcg cgagcacggc
     2041 tttaaaaacg gcgtcttttt tgcccaggct aaaggtcggc taattatcga cggtatcgag
     2101 gcgctgaaat ccgcgttctg gaatttctct tcattctcgc tggaaactgt cgctcaggag
     2161 ctattaggcg aaggaaaatc tatcgataac ccgtgggatc gaatggacga aattgaccgc
     2221 cgtttcgccg aagataaacc tgcgctggca acttataacc tgaaagattg cgagctggtg
     2281 acgcagatct tccacaaaac tgaaatcatg ccatttttac tcgaacgggc aacggtgaac
     2341 ggcctgccgg tggaccgaca cggcggttcg gtggcggcat ttggtcatct ctattttccg
     2401 cgaatgcatc gcgctggtta tgtcgcgcct aatctcggcg aagtgccgcc gcacgccagc
     2461 cctggcggct acgtgatgga ttcacggcca gggctttatg attcagtgct ggtgctggac
     2521 tataaaagcc tgtacccgtc gatcatccgc acctttctga ttgatcccgt cgggctggtg
     2581 gaaggcatgg cgcagcctga tccagagcac agtaccgaag gttttctcga tgcctggttc
     2641 tcgcgagaaa aacattgcct gccggagatt gtgactaaca tctggcacgg gcgcgatgaa
     2701 gccaaacgcc agggtaacaa accgctgtcg caggcgctga aaatcatcat gaatgccttt
     2761 tatggcgtgc tcggcaccac cgcctgccgc ttcttcgatc cgcggctggc atcgtcgatc
     2821 accatgcgtg gtcatcagat catgcggcaa accaaagcgt tgattgaagc acagggctac
     2881 gacgttatct acggcgatac cgactcaacg tttgtctggc tgaaaggcgc acattcggaa
     2941 gaagaagcgg cgaaaatcgg tcgtgcactg gtgcagcacg ttaacgcctg gtgggcggaa
     3001 acgctgcaaa aacaacggct gaccagcgca ttagaactgg agtatgaaac ccatttctgc
     3061 cgttttctga tgccaaccat tcgcggagcc gataccggca gtaaaaagcg ttatgccgga
     3121 ctgattcagg agggcgacaa gcagcggatg gtgtttaaag ggctggaaac cgtgcgcacc
     3181 gactggacgc cgctggccca gcagtttcag caggagctat acctgcgcat cttccgcaac
     3241 gagccatatc aggaatatgt acgcgaaacc atcgacaaac tgatggcggg tgaactggat
     3301 gcgcgactgg tttaccgtaa acgccttcgc cgtccgctga gcgagtatca gcgtaatgtg
     3361 ccgcctcatg tacgcgccgc tcgccttgcc gatgaagaaa accaaaagcg tggtcgcccc
     3421 ttgcaatatc agaatcgcgg caccattaag tacgtatgga ccaccacagg cccggagccg
     3481 cctggactac caacgttcac cactggatta cgaacactat ctgacccgcc agctacaacc
     3541 cgtggcggag ggaatactcc cttttattga ggataatttt gctacactta tgaccgggca
     3601 acttgggcta ttttgagcaa aaaaaagagt tcgccagata ccattttgat gcgtgacgaa
     3661 tgctttgcca tccagtacca tagcgccctt tccattcctg gacctgaata acaccactac
     3721 ctcataagca cggtagcggg tggttattgc ctgcaattaa agatatagag ccgaacacat
     3781 atgcctttta cacttggtca acgctggatc agcgatacag aaagcgaatt gggacttgga
     3841 accgttgtcg cggtggatgc gcgaactgtc actttacttt tcccatctac tggtgaaaac
     3901 cgtctgtacg cacgcagtga ttcccccgtg acccgcgtga tgttcaaccc tggtgatacc
     3961 attaccagcc atgacggctg gcagatgcaa gtcgaagaag taaaagaaga aaatggcttg
     4021 ctgacctata tcggtactcg cctggatact gaagaggtcc ggcgtagccc tgcgtgaagt
     4081 tttccttgat agcaaactgg tgttcagcaa accgcaggca ccgtctgttt gccgggcaga
     4141 ttgaccgtat ggaccgcttt gcgctgcgtt atcgcgcgcg taaatattcc agcgaacagt
     4201 tccgtatgcc gtacagcggc ctgcgcggtc agcgtaccag cctgatccgc atcagctcaa
     4261 catcgctcat gatgttggtc gccgccacgc gccgcgcgtc ctgctggctg acgaagtggg
     4321 tttagggaaa accattgaag ccgggatgat cctgcatcag caactgctct ctggcgctgc
     4381 tgaacgtgtg ctaattatcg tcccggaaac cttacagcat cagtggctgg tagaaatgct
     4441 gcgccgtttc aacctgcgct ttgcgctatt tgatgatgag cgttatgccg aagctcagca
     4501 cgatgcttac aacccgtttg acaccgtgaa gcggcgcacg aaaaacgcga aagcgtttca
     4561 cgataaatgc gaaaacttta gctttcgcgc ttcaaatgaa acagatgtat taattactgc
     4621 tttttattca ttacatgggg atccccgggt accgagctcg aattcc
//