HDTenEightyP/NetNews huggingface dataset:
From: proberts@castle.ed.ac.uk (P Roberts)
Newsgroups: bionet.general
Subject: pUC119 : Info wanted.
Date: 19 Nov 92 10:36:41 GMT
Distribution: bionet
Organization: Edinburgh University

I'm looking for information on the plasmid pUC119.  None of the
databases to which I have access have heard of it.  Does anyone out
there have any information they could let me have?  I would like to know
things like marker (presumably ampicillin), polylinker and any other
bits and bobs that it has.  A complete sequence would be great if
someone has a copy on-line that they could mail me.
  Thanks very much for any help you can offer.

          Paul (proberts@uk.ac.ed.castle)
Xref: sparky bionet.general:1909 bionet.software:2150
From: cherry@OPAL.MGH.HARVARD.EDU
Newsgroups: bionet.general,bionet.software
Subject: Re: NCBI (especially Kabat) databases
Date: 21 Nov 92 15:27:06 GMT
Reply-To: cherry@OPAL.MGH.HARVARD.EDU
Distribution: usa
Organization: Molecular Biology - Mass Gen Hospital

>In article <1992Nov20.022102.12238@Princeton.EDU>, lpcasson@phoenix.Princeton.EDU (Lawrence P. Casson) writes:
>>PROBLEM: I would like to retrieve complete mouse T-lymphocyte receptor
>>variable domain sequences, and avoid having to deal with the small
>>fragments that constitute many of the GenBank entries.
>>
>>I'm assuming that the Kabat and Wu database is the best place to start.

I do not know of anyone that has indexed the Kabat databases and
provides a mechanism to retrieve individual sequences.

I have converted the Kabat amino acid database into GenPept format so
that the GCG utilities can be used. I used the Kabat 5.0 (beta) amino
acid database.  It is available via anonymous ftp from
amber.mgh.harvard.edu as follows:

cd genbank
get kabat.genpept

Once on your system you can use: GENBANKTOGCG/GENPEPT to convert this
text file into a GCG formated database. After then running SEQCAT you
can use STRINGS and FETCH to retrieve the specific sequences you want.

Mike Cherry
From: joe@evolution.u.washington.edu (Joe Felsenstein)
Newsgroups: bionet.general
Subject: Re: class-L list?
Summary: e-mail address of class-l mailing lust
Date: 17 Nov 92 04:02:00 GMT
Organization: University of Washington, Seattle

In article <1992Nov16.211325.130@news.cs.brandeis.edu> jeremy john ahouse <ahouse@hydra.rose.brandeis.edu> writes:
>I am trying to subscribe to the list server called Class-L, 
>        I have the addresses: 
>        LISTSERV@SBCCVM and CLASS-L%SBCCVM.BITNET@CUNYVM.CUNY.EDU
>        but neither of these works from my internet site.

I think it's  class-l@ccvm.sunysb.edu

-----
Joe Felsenstein, Dept. of Genetics, Univ. of Washington, Seattle, WA 98195
 Internet:         joe@genetics.washington.edu     (IP No. 128.95.12.41)
 Bitnet/EARN:      felsenst@uwavm
Newsgroups: bionet.general
From: bfrank@aardvark.ucs.uoknor.edu (FRANK,BART)
Subject: Reference for pUC118/119
Originator: usenet@midway.ecn.uoknor.edu
Date: Mon, 23 Nov 1992 16:41:00 GMT
News-Software: VAX/VMS VNEWS 1.41    
Organization: University of Oklahoma - University Computing Services

pUC118 & pUC119 were described quite some time ago. They are for the 
most part similar to pUC18 & pUC18 but also conatin and M13 intergenic
region. These vectors were described by Vieira & Messing in 1987, Methods
of Enzymology, 153:3-11.

Bart Frank
Internet: BFRANK@AARDVARK.UCS.UOKNOR.EDU
Newsgroups: bionet.general
From: fbignone@alchemy.chem.utoronto.ca (Franco Bignone)
Subject: info request IGD database
Organization: University of Toronto Chemistry Department
Date: Wed, 18 Nov 1992 17:29:14 GMT


I am currently looking for information about a Human Database:
IGD, Integrated Genomic Database, that I understand should be related
to the Human Genome project, does anyone have more information about it?
Who developed it, where is available by ftp etc.
Tx

---------------------------------------------------------------------------
Dr. Franco A. Bignone				    home (416)932-1706
Physical Chemistry Theory Group			    bus  (416)978-5325
Dept. of Chemistry, University of Toronto,	    fax  (416)978-8775
St. George St., Toronto, Canada M5S1A1.
---------------------------------------------------------------------------
Newsgroups: bionet.general
From: harper@convex.csc.FI (Rob Harper)
Subject: Re: info request IGD database
Organization: Finnish Academic and Research Network Project - FUNET
Date: Fri, 20 Nov 92 11:46:21 GMT

In <1992Nov20.105002.14351@crc.ac.uk> gwilliam@crc.ac.uk 
(Gary Williams x3294) writes: about IGD

>   TOTAL           278,099 objects / 173 Mbytes of disk space

The acedb and the aatdb folks make their databases available for
ftp over the net. Is IGD available from any site?


--
 ~ Rob Harper                    ~ E-mail:          harper@convex.csc.fi    
 ~ Finnish State Computer Centre ~ Molbio/software: harper@nic.funet.fi
 ~ P.O. Box 40                   ~ Telephone:       +358 0 457 2076
 ~ SF-02101 Espoo Finland        ~ Fax:             +358 0 457 2302
Newsgroups: bionet.general
From: D.G.McSweeney@massey.ac.nz (D.G. McSweeney)
Subject: REQUEST:  Ladybird Information
Organization: Massey University, Palmerston North, New Zealand
X-Reader: NETNEWS/PC Version 2c
Date: Mon, 23 Nov 92 03:18:15 GMT

I have been lumbered with the task of establishing a culture of
the two-spotted ladybird (Adalia bipuctata).  It is a predatory
ladybird that feeds mainly on aphids.

My brief is to establish a breeding colony of at least 400
adults.

Any advice, criticism, warnings gratefully received, especially
any information on artificial diets.


Thanks,

          David McSweeney

E-mail `D.G.McSweeney@massey.ac.nz'
Voice 0064 6 356-9099 (x7843)
From: proberts@castle.ed.ac.uk (P Roberts)
Newsgroups: bionet.general
Subject: Re: pUC119 : Info wanted.
Date: 20 Nov 92 11:56:19 GMT
Organization: Edinburgh University

>> {request for info about pUC119 deleted}

wetsel_r@wums.wustl.edu writes:
>Forgive the stupid question... are you sure it's pUC119 as in *119*????  
>I've heard of pUC19, as in *19*, but not 119.  If it is in fact pUC19 that 
>you need, I think I can provide it to you but having been in mol-bio for 5 
>years, I've never heard of pUC119.

>Best regards,

>David L. Haviland
>haviland@kids.wustl.edu

  Yes, I am sure it is pUC119 and not pUC19.  That is why I requested
help.  I too had never come across this particular plasmid despite
having used various other pUC vectors (including pUC19).  A kind person,
whose name escapes me at the moment, has informed me via e-mail that
pUC119 is a derivative of pUC19 that contains a phage origin of
replication and suggested that I try some of the original Messing papers
where they think it was mentioned.  Many thanks for this information; I
would, however, still be interested in acquiring the sequence if anyone
has it on-line.

           Paul
From: wetsel_r@wums.wustl.edu
Newsgroups: bionet.general
Subject: RE: pUC119 : Info wanted.
Date: 19 NOV 92 16:50:44 GMT
Organization: MIT PLASMA FUSION CENTER
NNTP-Posting-Host: msnews.wustl.edu

In a previous article, proberts@castle.ed.ac.uk (P Roberts) wrote:
>I'm looking for information on the plasmid pUC119.  None of the
>databases to which I have access have heard of it.  Does anyone out
>there have any information they could let me have?  I would like to know
>things like marker (presumably ampicillin), polylinker and any other
>bits and bobs that it has.  A complete sequence would be great if
>someone has a copy on-line that they could mail me.
>  Thanks very much for any help you can offer.
> 
>          Paul (proberts@uk.ac.ed.castle)
----=
Paul:

Forgive the stupid question... are you sure it's pUC119 as in *119*????  
I've heard of pUC19, as in *19*, but not 119.  If it is in fact pUC19 that 
you need, I think I can provide it to you but having been in mol-bio for 5 
years, I've never heard of pUC119.

Best regards,

David L. Haviland
haviland@kids.wustl.edu
From: kengo@fl.lab.shionogi.co.jp (Kengo Kitadokoro)
Newsgroups: bionet.xtallography
Subject: poteinase inhibitors (In Japanese/Kanji)
Date: 18 Nov 92 07:57:49 GMT
Organization: Shionogi Research Lab.,Shionogi & Co.,Ltd.

Hello colleague,


 I would like to know the information about the structure of
Antithrombin and TIMP.

 Has the 3-D structure of Antithrombin-III analysis completly
finished ?

 And is the TIMP commercially availiable ?  Does any group solve the
3-D structure of TIMP ?
~
Is there any papers about this ?

Many Thanks about your sending me informations.

Best regards,


Kengo Kitadokoro
   SHIONOGI RESEARCH LABORATORIES
   12-4,Sagisu 5-chome,Fukushima-ku
   Osaka 553,Japan
   Tel: 06-458-5861 ; FAX: 06-458-0987

   e-mail address : kengo@fl.lab.shionogi.co.jp
Newsgroups: bionet.xtallography
From: danj@welchgate.welch.jhu.edu (Dan Jacobson)
Subject: Re: poteinase inhibitors (In Japanese/Kanji)
Organization: Johns Hopkins Univ. Welch Medical Library
Date: Mon, 23 Nov 1992 22:37:54 GMT

In article <1521@fl.lab.shionogi.co.jp> kengo@fl.lab.shionogi.co.jp (Kengo Kitadokoro) writes:
>Hello colleague,
>
>
> I would like to know the information about the structure of
>Antithrombin and TIMP.
>
> Has the 3-D structure of Antithrombin-III analysis completly
>finished ?
>
> And is the TIMP commercially availiable ?  Does any group solve the
>3-D structure of TIMP ?

A search of the Protein Data Bank (PDB) does not find Antithrombin or
TIMP - There are  is just one protein which is found with a query
for Metalloproteinase -

THERMOLYSIN (E.C.3.4.24.4) - which is present as a complex with a variety 
of compounds.

Best of luck,

Dan Jacobson

danj@welchgate.welch.jhu.edu
danj@chablis.gwu.edu
From: T80SMS1@NIU.bitnet
Newsgroups: bionet.population-bio
Subject: Job announcement
Date: 16 Nov 92 15:00:00 GMT
Distribution: bionet


            POSITION ANNOUNCEMENTS

Northern Illinois University anticipates a
tenure-track ASSISTANT PROFESSOR position for
fall, 1993.  A Ph.D and postdoctoral experience are
required.  Each candidate will be expected to
develop a vigorous externally funded research
program, direct graduate (M.S. and Ph.D) students,
and teach introductory courses for majors and non-
majors.

EVOLUTIONARY BIOLOGIST:  The candidate should be
organismally oriented and have research experience
dealing with evolutionary aspects of physiology or
development.  The candidate's research program will
complement the department's current strengths in
evolutionary biology and ecology.  Teaching
obligations will include courses in evolution and
ecology and a course in the candidate's area of
expertise.

  Send a letter of interest, curriculum vitae,
brief statement of research goals and teaching
goals, representative reprints, and three letters
of reference by December 31, 1992 to:  Dr. Marvin
Starzyk, Chair, Department of Biological Sciences,
Northern Illinois University, DeKalb, IL 60115-
2861.  Telephone:  815-753-1753.  Northern Illinois
University is an Affirmative Action/Equal
Opportunity Employer.
From: T80SMS1@NIU.bitnet
Newsgroups: bionet.population-bio
Subject: Re: genetic correlations
Date: 18 Nov 92 15:04:00 GMT
Distribution: bionet

>From: Draye@GENA.UCL.AC.BE
>Subject: genetic correlations
>Now, suppose that you derive your genetic correlations from mean genotypic
>values of strains of Drosophila melanogaster from different geographic origins.
>If you find a significative correlation by this way, does it implie that the
>same correlation should appear inside each of the populations?

Of course not.  The correlation across populations can easily be
of opposite sign to the correlation within population.  Actually
the original message is ambiguous.  If several lines per
population were measured, then the resulting genetic correlation
contains both among- and within-population elements.  A simple
nested analysis of covariance can be used to tease appart the
two factors.

*+*+*+*+*+*+*+*+*+*+*+*+*+*+*+*+*+*+*+*+*+*+*+*+*+*+*+*+*+*+*+*+*+*
* Samuel M. Scheiner                 +                            *
* Department of Biological Sciences  +    The purpose of an       *
* Northern Illinois University       +    education is to turn    *
* DeKalb, Illinois 60115             +    an empty mind into      *
* Phone:  (815) 753-7847             +    an open one.            *
* Fax:    (815) 753-0461             +       Malcolm Forbes       *
* Bitnet: t80sms1@niu                +                            *
*+*+*+*+*+*+*+*+*+*+*+*+*+*+*+*+*+*+*+*+*+*+*+*+*+*+*+*+*+*+*+*+*+*
From: Draye@gena.ucl.ac.be
Newsgroups: bionet.population-bio
Subject: genetic correlations
Date: 18 Nov 92 08:00:43 GMT
Distribution: bionet
Organization: Universite Catholique de Louvain
X-Acknowledge-To: <GENA2@BUCLLN11.BITNET>
Original-To: pop-bio@uk.ac.daresbury


Some stuff for your reflexion this night...

Consider genetic correlations between components of early fitness and late
fitness. The strengh  of natural selection on the second should be low
(compared to the other).
Now, suppose that you derive your genetic correlations from mean genotypic
values of strains of Drosophila melanogaster from different geographic origins.
If you find a significative correlation by this way, does it implie that the
same correlation should appear inside each of the populations?

If you have some idea, or reference, or...
Thanks,

Xavier DRAYE
Unite de Genetique
LOUVAIN LA NEUVE
BELGIUM
E-Mail: gena2@buclln11
From: nicky@akira.fdn.org (Jean-Philippe NICAISE)
Newsgroups: bionet.immunology,sci.med
Subject: Immunology Question on Complement 4
Date: Sun, 22 Nov 92 20:18:26 MET
Organization: French Data Network
Reply-To: jr92@calvacom.fr
Distribution: world
X-Mailer: uAccess - Macintosh Release: 1.6v1

Hello all,

I am writing this article for a friend who suffer from an acquired
immune deficiency that is NOT HIV AIDS (that is it doesn't comes from
a virus and is not transmissible).

Is there now a specific serum that could make up (in a isolated manner)
a deficiency of the complement 4 (that is without PPSB). If so, what
are the consequences ? Is there a risk to create some antibodies ?

Please answer directly through e-mail to 'JR92@calvacom.fr' (Jerome
Rabenou) since we don't have access to this newsgroup except for writing.
Jerome is not the people suffering from the deficiency but a close
friend to her.

Thanks for any help,
== nicky.

    Jean-Philippe Nicaise -- French Data Network Board -- Paris, France
    E-Mail: nicky@fdn.org, nicky@etca.fr -- Fax: (33-1) 43.57.30.97
From: U196CFA@CSI.IT
Newsgroups: bionet.immunology
Subject: antigenic peptide data bank
Date: 19 Nov 92 18:23:00 GMT
Distribution: bionet
X-Envelope-To: immuno@daresbury.ac.uk
Original-To: immuno@uk.ac.daresbury
X-Vms-To: IN%"immuno@daresbury.ac.uk"

Hello, 
does anyone know if an antigenic peptide, and related poly or monoclonal
antibody, databank exist ??, if so, where is it available?
Thanks,
			Franco Dosio
			University of Turin
			Istit. Chimica Farmaceutica Applicata
			Italy
			internet u196cfa@csi.it  
				 dosio@mvx36.csata.it
From: weed@venus.ycc.yale.edu
Newsgroups: bionet.announce,bionet.general
Subject: Materials availability for blind students interested in biology
Date: 21 Nov 92 00:32:48 GMT
Organization: Yale Computer Center (YCC)

I am a blind undergraduate at Yale, studying both Political Science and
Biology.  Recently, the point was made to me that the extreme availability of
materials which I have here may not be replicated at other, less well-endowed,
institutions.  Due to Yale's ability to pay for such things, it has not been
problematic to transfer materials from printed to electronic format , when
these conversions have been necessary.
However, for many blind students interested in studying in the sciences,
materials transfer is slow, delayed by the natural problems which taping (using
audio cassettes), of materials often bring.  The ability to get textbooks, (and
especially journals), transferred to audio-available formats is there, but
usually such transfers require money to complete.  I would be very much
interested in working with professors who are the authors of textbooks to see
if their books could be made available to blind students via either FTP or
through Recording For The Blind's "E-text" service which attempts to get books
from publishers in ASCII so that students can read the books on their home
computers.
Also, anyone who is on the review staff of some of the major journals,
particularly "Cell", and the journals relating to research in molecular
biochemistry, I would much like to discuss conversions and availabilities for
my own needs in graduate school, which I expect to enter in September of 1993,
studying to work in the public policy area as it relates to research in
cellular, developmental, molecular biochemistry, and genetics-related biology.
Any assistance/interest in the above would be greatly appreciated.
MattHew Weed,
Yale/Silliman 93.
From: kristoff@net.bio.net (David Kristofferson)
Newsgroups: bionet.announce
Subject: bionet.virology passes: 94 YES, 1 NO
Date: 23 Nov 92 21:57:21 GMT
Distribution: bionet
Organization: BIOSCI International Newsgroups for Biology

Voting is closed on bionet.virology, and the group has been approved
by a vote of 94 to 1.  We'll get the wheels in motion to establish the
newsgroup and mailing list here and at Daresbury, and then make an
announcement in this forum when it is ready for subscriptions and
operation.

				Sincerely,

				Dave Kristofferson
				BIOSCI/bionet Manager

				kristoff@net.bio.net
From: kristoff@genbank.bio.net (Dave Kristofferson)
Newsgroups: bionet.announce
Subject: Ensuring that your news software receives ALL of the bionet groups
Date: 21 Nov 92 03:00:19 GMT

I am passing along this recent message from my colleague Kenton which
was posted to BIOFORUM/bionet.general.  Kenton posts monthly messages
which can be used by the news software to update your local
distribution if new BIOSCI/bionet groups are created.  Some sites may
not be using this information properly and thus might not see new
groups.  If you can't find newsgroups locally that you see listed in
our monthly info sheet posting, please pass this along to your systems
administrator.

				Sincerely,

				Dave Kristofferson
				BIOSCI/bionet Manager

				kristoff@net.bio.net

----------------------------------------------------------------------

From: shibumi@net.bio.net (Kenton A. Hoover)
Subject: Re: class-L list?
Date: 18 Nov 92 22:59:58 GMT

ahouse@hydra.rose.brandeis.edu (jeremy john ahouse) writes:
>A second question.  I have access to newsgroups, and in
>fact can see a number of bionet groups, but not bionet.info-theory.  
>So I subscribe via email.  Do others have this situation?

Messages are sent out every month to the 'control' and 'news.admin.misc'
groups (to sites that take distribution 'bionet') to verify that they are
taking all of the bionet newsgroups.  If you have any further questions
about this, please mail me directly, or discuss this issue with your
local systems administration staff (you may also pass my address along to
your local systems staff, and I can consult with them on your problem).


| Kenton A. Hoover                                 |      shibumi@net.bio.net |
| BIOSCI Network Administrator                     |                          |
| BIOSCI/IntelliGenetics, Inc.                     |          +1 415 962 7300 |
|=========== net.bio.net -- The New Home of the bionet Newsgroups ============|
|         The only thing necessary for the triumph of evil is for good        |
|                men to do nothing.  -- Edmund Burke (1729-1797)              |
Newsgroups: bionet.neuroscience
From: crystal@glia.biostr.washington.edu (Crystal)
Subject: Re: Vision problem and Virtual Reality
Organization: University of Washington
Date: Sat, 21 Nov 1992 03:50:36 GMT

In <19921120.133923.554@almaden.ibm.com> simonofb@wmavm7.VNET.IBM.COM writes:

>In <crystal.722283589@glia> Crystal writes:
>>
>>physical clumsiness...
>>frequent headaches.  And no, he can not deal with 3-D,
>>nor use binoculars
>>or microscopes with any success.
>>

>No on the clumsiness for me, yes on the headaches.  I can use
>binoculars and microscopes only if I close one eye.

He "one-eyes" things, too.

>>While amblyopia (lazy eye) can be a contributing factor to strabismus,
>>the eye-patch idea has not been proven to be a cause of later
>>strabismus.

>So could be genetic, could be eye patch.  I did indeed have an eye
>patch, but, being alive, I also have genetics.

????
I'm afraid I just can't parse your comment...

>>Her condition was treated non-surgically, but I
>>forget the details now.

>You are saying that there might be a cure of rmy lack 'o 3D vision?

No, I said "treated", not cured.  Treated to the point of decent functionality
in daily life. 

>Robert Simonoff
>simonofb@wmavm7.vnet.ibm.com

Crystal
   ;>
From: astlab3@uci.edu
Newsgroups: bionet.neuroscience
Subject: Re: (none)
Date: 17 Nov 92 19:34:30 GMT
Organization: Bio Sci

In article <2B080857.3179@news.service.uci.edu> mundkur@falcon.eng.uci.edu (Prashanth Mundkur) writes:


>       Some questions for which I am looking for answers are
>1) How do you conduct an experiment that traces the path of an axon for over
>50 cms? 
>2) How do you say that a neuron responds to a certain stimulus? What exactly is
>involved when one places electrical electrodes on a cell membrane?
>3) Drawing conclusions about a neuron's functional behaviour from brain lesion
>experiments surely is very error-prone. What are the mistakes one should not
>make when drawing conclusions from such experiments?
>4) When one sets up a "mathematical" model of the operation of a set of neurons
>in a particular part of the nervous system, what does one normally take into 
>account, and what does one assume to be irrelevant to the modeling objective?
[comments deleted]
>Thanks for any advice and/or pointers to literature.
>--Prashanth.

	These are some very broad questions, but I will attempt to get you
headed in the right direction.
1)  There are many techniques for tracing the length of an axon.  One of
	first used is to inject horseradish peroixdase into the synapse
	where the axon terminates.  This substance is then taken up into
	the cell and through retorgrade transport marks the whole cell.
	For more info, look into "Neurobiology" by G. Shepard.
2)  Again there are many techniques (ie. voltage clamp, patch clamp, etc.)
	In voltage clamp, the cell is impaled by 2 electrodes, and by
	supplying current, the cell's voltage is 'clamped'.  The current
	supplied to keep the cell at the chosen voltage is equal to 
	the current passing through the membrane by ionic flux.
	Good references are: "Ionic channels of excitable membranes" by
	Hille, and "Physiology of excitable cells" by Aidley.
3)  You are correct in saying that the interpretation of lesion studies
	is often subject to much scrutiny.  This technique is extensively
	used, and there are many methods for producing both reversible
	and non-reversible lesions.  Try "Principles of Neural Science"
	by Kandel, Schwartz, and Jessel.  
4)  Currently the connectionist paradigm is the most popular for modelling
	the functioning of the brain.  The connectionist's bible is 
	"Parallel distributed processing" by some guys at San Diego that
	 I can't recall right now.

	I hope that this will give you a good start.  To answer most
of your questions in detail will require a great deal of reading.  Your
best bet is to begin with "Principles of Neural Science", and look for
more details elsewhere if necessary.  Good luck.

***************************************************************************
Brooke Paul                                         * bpaul@darwin.
UCI- Department of Psychobiology                    *          bio.
Laboratory of Cellular and Molecular Neurobiology   *          uci.
Irvine, Ca.  92715                                  *          edu
*************************************************************************** 
of your	questions
From: wangr@ERE.UMontreal.CA (Wang Rui)
Newsgroups: bionet.neuroscience
Subject: Frog neurotoxin
Date: 17 Nov 92 21:43:04 GMT
Distribution: bionet

Dear fellows,
	I read two posts last month regarding the knowledge of the existence of neurotoxins subtracted from FROG.
	Does anyone can give me a summary of frog neurotoxin or other toxins from FROG?

	Thanks a million.


	Rui Wang

	Wangr@ere.UMontreal.ca