From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: S.thermophilus gene for replication protein (Rep A)
Date: 29 Dec 92 21:28:40 GMT
Distribution: bionet
Checksum: 12049 5

LOCUS       STRREPA      2093 bp ds-DNA             BCT       14-DEC-1992
DEFINITION  S.thermophilus gene for replication protein (Rep A)
ACCESSION   X65856
KEYWORDS    replication protein.
SOURCE      Streptococcus thermophilus DNA.
  ORGANISM  Streptococcus thermophilus
            Prokaryotae; Firmicutes; Firmibacteria; Gram-positive cocci;
            Streptococcaceae.
REFERENCE   1  (bases 1 to 2093)
  AUTHORS   Janzen,T., Kleinschmidt,J.A., Neve,H. and Geis,A.M.
  TITLE     Sequencing and characterization of pST1, a cryptic plasmid from
            streptococus thermophilus
  JOURNAL   FEMS Microbiol. Lett. 95, 175-180 (1992)
  STANDARD  full automatic
COMMENT     *source: strain=ST;
               Complete sequence of cryptic plasmid pST1
               From EMBL    entry STREPA;  dated 27-AUG-1992.
FEATURES             Location/Qualifiers
     misc_feature    1..2093
                     /note="cryptic plasmid pST1"
     -35_signal      182..187
     -10_signal      205..210
     RBS             246..253
     CDS             303..1250
                     /product="replication protein (RepA)"
                     /codon_start=1
                     /translation="MTDFKKQGEILVDKNSRGKERDWRGRKILSLKLADIFKELQYKK
                     TFVERVISCGDTLQFIQNQDGNLKLYQTYFCKNKLCPMCNWRRSMKYSYQTSRIVDEA
                     IKQSPKGRFLFLTLTVKNVEGEALNSTISQLTKSFDRLFKRAKVQRNLLGYLRSVEVT
                     HNENNKTYHPHIHVLMMVRPSYFQSKKDYITQKEWSDMWSNSLKVDYTPMIDIRTVKE
                     TGKGLRGAVLETAKYPTKPIKLDVENKQVVDDLYNGLYRKRQLGYGGLFKAIKKQLAL
                     DDAENGDLVHTSDDKENISKGTEIVAIWNANKQNYYLKK"
BASE COUNT      706 a    323 c    402 g    662 t
ORIGIN      
        1 gctagcgttt tgagagtgaa ttattgctga ataaaaagag ggctgaaagc caaaaaaagc
       61 agtcgcaaga ctgctttctt cttatcttga tactaataga aataattcac ggggaaattt
      121 caaaccccgt aggttctaaa acccttgtca ctactggttt tttgggcaaa aaaaaacccg
      181 tttcatttct gaaaggtttc gtgttataat tttatgtgtg tcgaagggga aaaatataaa
      241 tacacgaaag gagaactacg gatttttttg catcgcaaat taatttgtat atatagtata
      301 gcatgacgga ttttaaaaaa caaggggaaa ttcttgtaga taagaacagt cgaggaaaag
      361 aacgagactg gcgaggacga aaaatattaa gtttgaaatt agctgatatt tttaaagaat
      421 tgcagtacaa aaagactttt gttgaaagag taatatcgtg tggtgatact ttacaattta
      481 ttcaaaatca agacggtaac ctaaagctat atcaaaccta tttttgtaaa aacaagctat
      541 gtccgatgtg taattggaga cggtcaatga aatattctta tcaaacatca aggattgtag
      601 atgaggcaat aaaacaaagc cctaaaggac gctttctctt tctgacattg actgttaaga
      661 acgtcgaggg agaagccttg aatagtacga ttagtcagct cacaaaatca tttgatcgtt
      721 tatttaagcg tgctaaagtt caaagaaatc tgttgggata tttgcgttct gttgaagtga
      781 cccacaatga aaataacaag acatatcacc ctcatattca tgttttgatg atggtgagac
      841 ctagttattt tcagtcaaaa aaagattata tcactcaaaa agagtggagc gatatgtggt
      901 caaattcatt gaaagttgat tatactccta tgattgatat tagaacagtc aaagagactg
      961 gcaaaggact gcgaggggca gtcttggaaa cggcaaaata tccaacgaaa ccgattaagc
     1021 ttgacgtaga gaataagcaa gtggtagatg atttatataa cggtctgtat cgaaaacgtc
     1081 agctaggata cggtggttta tttaaagcta tcaaaaaaca actagcacta gatgacgcag
     1141 aaaatggtga cttggtgcat acgtctgacg ataaagaaaa catatcaaag ggtacagaaa
     1201 ttgttgctat ttggaatgcc aacaagcaaa attattattt gaaaaaataa agctcctttt
     1261 ccgaggagct tttttaaatg gtatagacat tttatgaaaa ccctcaaaat ttgtgcgtga
     1321 agtaagaaaa caccttgtcg tggctctcct aaagcccata ataggggcgg gggtcgtttt
     1381 taaactggtc taggggaaat atactgcccc ttatataaaa tcgtgctacg ggcgttttat
     1441 aggggtttaa cgacgtctct cttttagttt accaactgtt ttataattag ggcgttttat
     1501 accaacgttt tccaacaatt agggcgtctt tcttttgaat tagtgattct gttgccgtgt
     1561 aactcctaat cgttattagt taactttcta gttatcacgt ttgtaaccac cgtaatcact
     1621 tatatataaa ggttttcagt cactttagtt acctagttat atgttatttt ggattctcta
     1681 tctctatata ttatatatat ttatttattt atttatttta actttatata aaaagtagta
     1741 attaggtaac taaagcaacc aaacccttta tttttcaatg ggtttcacgg ttactggttg
     1801 cattaacttt atattaacta attaaccata ataactacac aggtcacctc atactaccat
     1861 agacactact attagtgtca aagatgtgtc acagtgtcct aacaagaagt acagtcgttt
     1921 tatgccgtga aagttatttg acaataagct aggtcaagtt agactggttt aggcgtgtca
     1981 gtctgaattt tgttcagctg accgacaaac caaagactaa cgacctaggt ttcttgttgt
     2041 caaataaacc atggaataaa tagacttaag tttgaatgtt gtttgattta gat
//