From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Rat peptidylglycine alpha-amidating monooxygenase (PAM) gene, exon
Date: 29 Dec 92 19:50:50 GMT
Distribution: bionet
Checksum: 41449 7

LOCUS       RATPAM16     1646 bp ds-DNA             ROD       08-DEC-1992
DEFINITION  Rat peptidylglycine alpha-amidating monooxygenase (PAM) gene, exon
            16.
ACCESSION   L01682
KEYWORDS    alternative splicing;
            peptidylglycine alpha-amidating monooxygenase.
SEGMENT     16 of 27
SOURCE      Rattus norvegicus (strain Sprague-Dawley) (library: lambda Fix)
            male adult liver DNA.
  ORGANISM  Rattus norvegicus
            Eukaryota; Animalia; Chorqata; Vertebrata; Mammalia; Theria;
            Eutheria; Rodentia; Myomorpha; Muridae; Murinae.
REFERENCE   1  (sites)
  AUTHORS   Stoffers,D.A., Ouafik,L. and Eipper,B.A.
  TITLE     Characterization of novel mRNAs encoding enzymes involved in
            peptide alpha-amidation
  JOURNAL   J. Biol. Chem. 266, 1701-1707 (1991)
  STANDARD  full automatic
REFERENCE   2  (sites)
  AUTHORS   Eipper,B.A., Green,C.B., Campbell,T.A., Stoffers,D.A.,
            Keutmann,H.T., Mains,R.E. and Ouafik,L.
  TITLE     Alternative splicing and endoproteolytic processing generate
            tissue-specific forms of peptidylglycine alpha-amidating
            monooxygenase (PAM)
  JOURNAL   J. Biol. Chem. 267, 4008-4015 (1992)
  STANDARD  full automatic
REFERENCE   3  (bases 1 to 1646)
  AUTHORS   Ouafik,L., Stoffers,D.A., Campbell,T.A., Johnson,R.C.,
            Bloomquist,B.T., Mains,R.E. and Eipper,B.A.
  TITLE     The multifunctional peptidylglycine alpha-amidating monooxygenase
            gene: Exon/intron organization of catalytic, processing and routing
            domains
  JOURNAL   Mol. Endocrinol. 6, 1571-1584 (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     exon            51..365
                     /number=16
                     /gene="PAM"
     exon            51..1560
                     /number=16
                     /gene="PAM"
                     /note="for alternatively spliced PAM4"
     polyA_signal    1527..1532
                     /gene="PAM"
     polyA_site      1560
                     /gene="PAM"
     intron          order(L01681:325..651,1..50)
                     /number=15
                     /gene="PAM"
                     /note="11.6 kb gap"
     mat_peptide     join(L01669:24..143,L01670:71..128,L01671:187..274,
                     L01672:89..174,L01673:162..245,L01674:77..125,
                     L01675:163..230,L01676:227..307,L01677:67..143,
                     L01678:186..289,L01680:308..492,L01681:253..324,51..424)
                     /product="peptidylglycine alpha-amidating monooxygenase"
                     /gene="PAM"
                     /codon_start=1
                     /note="alternatively spliced PAM4 protein"
     mRNA            join(L01668:114..518,L01669:23..143,L01670:71..128,
                     L01671:187..274,L01672:89..174,L01673:162..245,
                     L01674:77..125,L01675:163..230,L01676:227..307,
                     L01677:67..143,L01678:186..289,L01680:308..492,
                     L01681:253..324,51..1560)
                     /product="peptidylglycine alpha-amidating monooxygenase"
                     /gene="PAM"
                     /note="alternatively spliced PAM4 mRNA"
     CDS             join(L01668:415..518,L01669:23..143,L01670:71..128,
                     L01671:187..274,L01672:89..174,L01673:162..245,
                     L01674:77..125,L01675:163..230,L01676:227..307,
                     L01677:67..143,L01678:186..289,L01680:308..492,
                     L01681:253..324,51..427)
                     /EC_number="1.14.17.3"
                     /gene="PAM"
                     /note="alternatively spliced PAM4 protein"
                     /product="peptidylglycine alpha-amidating monooxygenase"
                     /codon_start=1
                     /translation="MAGRARSGLLLLLLGLLALQSSCLAFRSPLSVFKRFKETTRSFS
                     NECLGTIGPVTPLDASDFALDIRMPGVTPKESDTYFCMSMRLPVDEEAFVIDFKPRAS
                     MDTVHHMLLFGCNMPSSTGSYWFCDEGTCTDKANILYAWARNAPPTRLPKGVGFRVGG
                     ETGSKYFVLQVHYGDISAFRDNHKDCSGVSVHLTRVPQPLIAGMYLMMSVDTVIPPGE
                     KVVNADISCQYKMYPMHVFAYRVHTHHLGKVVSGYRVRNGQWTLIGRQNPQLPQAFYP
                     VEHPVDVTFGDILAARCVFTGEGRTEATHIGGTSSDEMCNLYIMYYMEAKYALSFMTC
                     TKNVAPDMFRTIPAEANIPIPVKPDMVMMHGHHKEAENKEKSALMQQPKQGEEEVLEQ
                     GDFYSLLSKLLGEREDVHVHKYNPTEKTESGSDLVAEIANVVQKKDLGRSDAREGAEH
                     EEWGNAILVRDRIHRFHQLESTLRPAESRAFSFQQPGEGPWEPEPSGGASRISFTQKK
                     KCVKHCNPH"
BASE COUNT      485 a    340 c    350 g    470 t      1 others
ORIGIN      
        1 gaaagtaacc atctataaac actcgcgtct tctttctttc cccctcgcag gtgatttcta
       61 ttcactgctt tccaagctgc taggagaaag ggaagatgtt catgtgcaca agtataatcc
      121 tacagaaaag acagaatctg ggtcagacct ggtagctgag attgcaaacg tggtccagaa
      181 aaaggacctt ggtcggtctg acgccagaga aggtgcagag catgaggaat ggggtaatgc
      241 tatcctagtc agagacagga tccacagatt ccaccagcta gagtcaactc tgaggccagc
      301 tgagagcaga gctttctcgt tccagcagcc tggcgaaggc ccttgggaac cagaaccctc
      361 aggaggtgca tccaggattt cttttacaca aaaaaaaaaa tgtgtgaaac actgcaatcc
      421 acattagcaa aatctgaagg gaataaaaaa taatgggcag caaaactcac caatagaaaa
      481 gacttactgg aacaagtatg actcagattt aagctgtctc atatcacatt ctgttttgaa
      541 aagatttttt ttccttttat ttttgtgttg gtccagtact acacaaagtc aacatttatc
      601 cagcgagtca gttgaaagta ggaccacact tgagtcattt tggaaactat aatatgtgaa
      661 attcagagta ataaatcaac gttttccatt ggctcaatag gaagaaattg aaatttccaa
      721 acatcaccta taaatgccag ggtatatagt gctcgcacta tcttctccag taattaatgt
      781 cctctcaaaa gaatcagata ctctcaacat ggctacccaa ctgaatcgta aaataaaacc
      841 attttttgtg accgatttct taagttccat tatcagatgc tatcctaatg gaaacttgag
      901 tatacatttc tgaagggaat ctccagtaag tcaataatta tcagatttat ctttatttct
      961 agccaggatt ggcttccata attagagaac aatgccttat ncccttcagg tttctgtgcc
     1021 agcaagccat agacgcctca tctttgtctt taacgttgct gcggcctctg tgatattgat
     1081 tgtgtttttg cgtgacttgc taatgtggaa agctgtgtaa taaagcacgt ggagggagtc
     1141 tctaaaaaag taacagatat gcgtgcatgg cagatgtcca cgcgtgggct tggatgacac
     1201 aggctgccag ctcgatgttt tcaaattcag ctttgcagga agccatgaat tattcaacac
     1261 ccagctgatt tttatttata attgaaaaaa aatagcatgc agtgcctttg cccacatgga
     1321 agcctcaagt atcgtccgtg gggactggcc taatggtctg tatgaggtgt taagtggagc
     1381 ctacactttt aactcctcag attttaacag gctcttaact ggttccaatt ttgtctaata
     1441 taacggtttc tcaagaggtg atcctgggac ccttcttgct tgagaattgc ccaaggtgtt
     1501 taagaaaaac ctaagttctg tatcctatta aagagcaagt gaactggtgt ctggtgatgg
     1561 agtgacagat gtgcagttca ttgtgtctct acccatctca taacatagga gggtctgtgt
     1621 gagcctctga gctccaccgc gtgcgc
//