From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Pisum sativum serine hydroxymethyltransferase mRNA, complete cds.
Date: 28 Jul 92 10:07:43 GMT
Distribution: bionet
Checksum: 53337     5

LOCUS       PEASHMTA     1803 bp ss-mRNA            PLN       28-JUL-1992
DEFINITION  Pisum sativum serine hydroxymethyltransferase mRNA, complete cds.
ACCESSION   M87649 M87650
KEYWORDS    mitochondrial isoform; serine hydroxymethyltransferase.
SOURCE      Pisum sativum (variety birte) (library: gt11) leaf cDNA to mRNA.
  ORGANISM  Pisum sativum
            Eukaryota; Plantae; Embryobionta; Magnoliophyta; Magnoliopsida;
            Rosidae; Fabales; Fabaceae.
REFERENCE   1  (bases 1 to 1803)
  AUTHORS   Turner,S.R., Ireland,R., Morgan,C. and Rawsthorne,S.
  TITLE     Identification and localization of multiple forms of serine
            hydroxymethyltransferase in pea (Pisum sativum) and
            characterization of a cDNA encoding a mitochondrial isoform
  JOURNAL   J. Biol. Chem. 267, 13528-13534 (1992)
  STANDARD  full staff_entry
FEATURES             Location/Qualifiers
     CDS             22..1578
                     /EC_number="EC.2.1.2.1"
                     /product="serine hydroxymethyltransferase"
                     /gene="serine hydroxymethyltransferase"
                     /codon_start=1
                     /translation="MAMAMALRKLSSSVNKSSRPLFSASSLYYKSSLPDEAVYDKENP
                     RVTWPKQLNSPLEVIDPEIADIIELEKARQWKGLELIPSENFTSLSVMQAVGSVMTNK
                     YSEGYPGARYYGGNEYIDMAETLCQKRALEAFRLDPAKWGVNVQPLSGSPSNFQVYTA
                     LLKPHDRIMALDLPHGGHLSHGYQTDTKKISAVSIFFETMPYRLDESTGYIDYDQLEK
                     SATLFRPKLIVAGASAYARLYDYARIRKVCDKQKAVLLADMAHISGLVAAGVIPSPFD
                     YADVVTTTTHKSLRGPRGAMIFFRKGLKEVNKQGKEVFYDYEDKINQAVFPGLQGGPH
                     NHTITGLAVALKQATTPEYRAYQEQVLSNSSKFAKALSEKGYDLVSGGTENHLVLVNL
                     KNKGIDGSRVEKVLELVHIAANKNTVPGDVSAMVPGGIRMGTPALTSRGFVEEDFVKV
                     AEYFDAAVSLALKVKAESKGTKLKDFVEALQTSSYVQSEISKLKHDVEEFAKQFPTIG
                     FEKATMKYNK"
     sig_peptide     22..114
                     /note="mitochondrial target sequence"
                     /gene="serine hydroxymethyltransferase"
                     /codon_start=1
     mat_peptide     115..1575
                     /EC_number="EC.2.1.2.1"
                     /product="serine hydroxymethyltransferase"
                     /gene="serine hydroxymethyltransferase"
                     /codon_start=1
BASE COUNT      538 a    329 c    415 g    521 t
ORIGIN
        1 atggaccttt ctatctctaa catggctatg gcaatggcgc ttcgtaagct ttcatcttcc
       61 gtcaacaaat cttcacgtcc tctcttttct gcttcatctc tttactacaa gtcttctttg
      121 cctgatgaag ctgtttacga caaagagaat ccacgtgtta cgtggccaaa gcaattgaat
      181 tctccacttg aggttattga tcctgaaatt gctgatataa ttgagcttga aaaagctaga
      241 cagtggaagg ggctggaact gataccatca gagaatttta cttctttatc tgttatgcaa
      301 gctgttggct ctgtcatgac aaacaaatac agtgaaggtt atcctggggc aagatattat
      361 ggaggaaatg agtatattga catggcagaa acactatgcc agaagcgtgc cttggaagca
      421 tttcggttgg atcctgctaa atggggagtg aatgtgcagc ctctgtctgg ttcgccttca
      481 aactttcaag tttacactgc attgttgaaa cctcatgata gaatcatggc acttgatctc
      541 cctcatggag ggcatctttc tcatggatac cagaccgaca ccaaaaagat atctgcagtc
      601 tctatatttt ttgagacaat gccatatagg ttggatgaaa gcacaggata cattgactat
      661 gaccagctag agaaatcggc tacactcttc aggccaaagt taatagttgc cggtgctagt
      721 gcctatgcaa ggctgtatga ttatgcacgc atacgcaagg tttgcgataa acagaaagct
      781 gtgttgttag cagatatggc acatatcagt ggattggttg cagctggtgt tatcccttca
      841 ccttttgatt atgcagatgt agtgacaacc acaactcaca agtcacttcg cggcccacgt
      901 ggagctatga tatttttcag gaagggatta aaagaagtca acaaacaagg aaaagaggtg
      961 ttctatgact atgaggacaa aatcaatcaa gctgtcttcc ccggactgca aggtggtcct
     1021 cacaaccata ccattacagg tttagctgtt gcattgaagc aggctacaac tccagagtat
     1081 agagcatatc aagagcaggt tctcagcaat agctcaaagt ttgcaaaggc tctgagtgag
     1141 aaaggctatg atcttgtttc cggtgggact gagaatcatt tagttttggt gaatctgaag
     1201 aacaagggta ttgatgggtc cagagttgag aaagtgttgg aattagtaca tattgctgct
     1261 aataaaaaca cagttccagg agatgtgtct gctatggttc ctggtggcat caggatggga
     1321 actcctgcgc ttacttcaag gggatttgtt gaggaggatt tcgttaaggt agcagagtat
     1381 tttgatgcag ctgtgagttt ggccttgaag gttaaggcag aaagcaaagg aacaaaatta
     1441 aaggacttcg tggaagcatt gcagacatcc tcttacgtgc aatcagagat ttccaagctt
     1501 aagcatgatg ttgaggagtt tgcaaaacaa tttccaacca ttggttttga aaaagcaacc
     1561 atgaagtaca acaaataagt aaaagaaagt ttccatcagt ggctgttaac actatgatgg
     1621 tcaagccctt caaggtgatc agttttatgc ttaatggaag aggagattat atatagcaga
     1681 tgaaaatcat taggatggaa cttttttatg tatggaactt gcaaaacttg atcagatatc
     1741 ttcttgcaat gtatagatac atatatttat aatttattaa atgtgaattg cgacttgcga
     1801 gct
//