From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Tetrahymena pyriformis beta-tubulin gene 1 (beta-TT1).
Date: 24 Jul 92 10:10:19 GMT
Distribution: bionet
Checksum: 04645     6

LOCUS       TETTUBB1     2159 bp ds-DNA             INV       24-JUL-1992
DEFINITION  Tetrahymena pyriformis beta-tubulin gene 1 (beta-TT1).
ACCESSION   X12768
KEYWORDS    beta-tubulin; tubulin.
SOURCE      Tetrahymena pyriformis.
  ORGANISM  Tetrahymena pyriformis
            Eukaryota; Animalia; Protozoa; Ciliophora; Oligohymenophora;
            Hymenostomata; Hymenostomatida; Tetrahymenina; Tetrahymenidae.
REFERENCE   1  (bases 1 to 2159)
  AUTHORS   Barahona,I., Soares,H., Cyrne,L., Penque,D., Denoulet,P. and
            Rodrigues-Pousada,C.
  TITLE     Sequence of one alpha- and two beta-tubulin genes of Tetrahymena
            pyriformis
  JOURNAL   J. Mol. Biol. 202, 365-382 (1988)
  STANDARD  simple automatic
COMMENT     *source: strain=CGL; library=macronuclear DNA in pBR322; *source:
            clone=beta-TT1.  tag encodes Gln !
               EMBL features not translated to GenBank features:
               key        from     to       description
               PRM          15     24       TATA-box
               SITE         26     32       poly(AG) stretch
               SITE         53     53       pot. transcription start site
               SITE         61     61       pot. alt. transcription start site
               SITE         65     65       pot. alt. transcription start site
               SITE       1763   1763       transcript termination site
               SITE       1769   1769       transcript termination site
               SITE       1772   1772       transcript termination site
FEATURES             Location/Qualifiers
     CDS             131..1462
                     /note="beta-tubulin 1 (AA 1 - 143)"
                     /codon_start=1
                     /translation="MREIVHIQGGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQL
                     ERINVYYNEATGGRYVPRAILMDLEPGTMDSVRAGPFGQLFRPDNFVFGQTGAGNNWA
                     KGHYTEGAELIDSVLDVVRKEAEGCDCLQGFQITHSLGGGTGSGMGTLLISKVREEYP
                     DRIMETFSVVPSPKVSDTVVEPYNATLSVHQLVENADECMVIDNEALYDICFRTLKLT
                     TPTYGNLNHLVSAAMSGVTCCLRFPGQLNSDLRKLAVNLIPFPRLHFFMIGFAPLTSR
                     GSQQYRALTVPELTQQMFDAKNMMCAADPRHGRYLTASALFRGRMSTKEVDEQMLNVQ
                     NKNSSYFVEWIPNNIKSSICDIPPKGLKMAVTFVGNSTAIQEMFKRVAEQFTAMFRRK
                     AFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATAEEEGEFEEEEGEN"
BASE COUNT      672 a    500 c    366 g    621 t
ORIGIN
        1 atttttccga atagtaatat ttaagaagag aggggatttt gaaggaaaaa tcataatcaa
       61 ataaaaaaat aaaaaagttt gaaaaaaacc taaaaaaaaa aacaacaaaa atcttctaaa
      121 agcaaataaa atgagagaaa tcgttcacat ctagggtggt caatgcggta accaaattgg
      181 tgccaagttc tgggaagtca tttccgacga acacggtatc gaccccaccg gtacttatca
      241 cggtgattcc gatttgcaat tggaaagaat caacgtttat tacaatgaag ctactggtgg
      301 tagatacgtc cccagagcta tcttgatgga tttggaacct ggtaccatgg attccgtcag
      361 agctggtcct ttcggtcaac tcttcagacc tgataacttc gttttcggcc aaactggtgc
      421 tggtaacaac tgggctaagg gtcactacac tgaaggtgct gaattgatcg actccgtttt
      481 ggacgtcgtc agaaaggaag ctgaaggttg cgattgcctc caaggtttcc aaatcaccca
      541 ctccctcggt ggtggtactg gttctggtat gggtactctc cttatctcca aggtcagaga
      601 agaatatcct gatagaatca tggaaacttt ctccgttgtc ccctccccca aggtctccga
      661 taccgtcgtc gaaccctaca acgccaccct ttccgtccat caattggtcg aaaatgctga
      721 tgaatgtatg gtcatcgata acgaagctct ttacgatatc tgcttcagaa ccctcaagct
      781 caccaccccc acctacggta atcttaacca cttggtctct gctgccatgt ccggtgtcac
      841 ctgttgcctc agattccccg gtcaattgaa ctccgatctt agaaaactcg ccgtcaactt
      901 gattcccttc cctcgtctcc atttcttcat gattggtttc gctcccctta cctccagagg
      961 ttctcaacaa tacagagctc ttaccgtccc tgaattgacc caacaaatgt tcgatgccaa
     1021 gaacatgatg tgcgctgctg atcccagaca cggaagatac ttgactgcct ccgctctctt
     1081 cagaggtaga atgtccacca aggaagtcga cgaacaaatg ctcaacgtcc aaaacaagaa
     1141 ctcttcttac ttcgttgaat ggattcccaa caacatcaag tcctccattt gtgatatccc
     1201 ccccaagggt ctcaagatgg ccgtcacctt cgttggtaac tccaccgcca tccaagaaat
     1261 gttcaagaga gtcgctgaac aattcactgc tatgttcaga agaaaggctt tcttgcattg
     1321 gtacactggt gaaggtatgg acgaaatgga attcactgaa gctgaatcca acatgaacga
     1381 tctcgtctcc gaatatcaac aatatcaaga tgccaccgct gaagaagaag gtgaattcga
     1441 agaagaagaa ggtgaaaact gaggaaactg aataaaatcc taatcacaac tacctaccca
     1501 cttctttatt atttttactt aagcgtctta ctgttgttgt aatagtggag agaaatacac
     1561 ccaattaact ttaacaataa tatctataaa catcaatctt ttttctcaca tatatacaac
     1621 tctcacaatc aaaaactttt taaaaataat cgatatatac tcaaatacca tatatctctt
     1681 tttacaaaat agttcatata tacatattct tgcactcacc ccgcataacc atttttcaca
     1741 actcaaaatg tccaaaatcc cttcactcta catgttattc aattctaatt acctattttc
     1801 tttctttcat tcattcttag ttaatacctt gcatgctttc ttatgtgtta acaatgatct
     1861 atcttcaatt tgtgaagaaa aacaacagca aatcttgctg aaaactcgtt cgattagata
     1921 gagacagaga agacagtcac aacattgatt gagcgaatca gcttttttga aaaaagataa
     1981 tctttttcta tgaatgctta ctacactgtc tctctttcag agatgaatga atttcctctt
     2041 ttgtttcctt cagttctata acttttaatt aaaaattgct ttcaatttcc cttcctcctg
     2101 tatcatcaaa aaatcaaaaa gagaaagaga ggaaggaaaa agaagatatt ttcaagctt
//