From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human MUC18 gene mRNA, complete cds.
Date: 5 Sep 92 10:01:22 GMT
Distribution: bionet
Checksum: 14630     8

LOCUS       HUMMUC18B    3290 bp ss-mRNA            PRI       05-SEP-1992
DEFINITION  Human MUC18 gene mRNA, complete cds.
ACCESSION   M28882
KEYWORDS    MUC18 glycoprotein; cell adhesion molecule;
            immunoglobulin-like protein; integral membrane glycoprotein.
SOURCE      Human (isolate JuSo) melanoma (cell line Mel JuSo), cDNA to mRNA,
            clone zapy1, drop 1, drop 4, drop 4.2, drop 4.7, drop 4.11.
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 3290)
  AUTHORS   Lehmann,J.M., Riethmueller,G. and Johnson,J.P.
  TITLE     MUC18, a marker of tumor progression in human melanoma, shows
            sequence similarity to the neural cell adhesion molecules of the
            immunoglobulin superfamily
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 86, 9891-9895 (1989)
  STANDARD  simple staff_entry
REFERENCE   2  (sites)
  AUTHORS   Sers,C.
  JOURNAL   Unpublished (1992)
  STANDARD  full staff_review
COMMENT     Draft entry and printed sequence for [1] kindly submitted by
            J.P.Johnson, 06-OCT-89.
FEATURES             Location/Qualifiers
     CDS             8..1818
                     /note="MUC18 glycoprotein precursor"
                     /codon_start=1
                     /translation="MGLPRLVCAFLLAACCCCPRVAGVPGEAEQPAPELVEVEVGSTA
                     LLKCGLSQSQGNLSHVDWFSVHKEKRTSSSVCARARARANLGSTSKRLSLQDRGATLA
                     LTQVTPQDERIFLCQGKRLGPRSTASSSASTKLRMPNIQVNPLGIPVNSKEPEEVATC
                     VGRNGYPIPQVIWYKNGRPLKEEKNRVHIQSSQTVESSGLYTLQSILKAQLVKEDKDA
                     QFYCELNYRLPSGNHMKESREVTVPVFYPTEKVWLEVEPVGMLKEGDRVEIRCLADGN
                     PPPHFSISKQNPSTREAEEETTNDNGVLVLEPARKEHSGRYECQAWNLDTMISLLSEP
                     QELLVNYVSDVRVSPAAPERQEGSSLTLTCEAESSQDLEFQWLREETDQVLERGPVLQ
                     LHDLKREAGGGYRCVASVPSIPGLNRTQLVKLAIFGPPWMAFKERKVWVKENMVLNLS
                     CEASGHPRPTISWNVNGTASEQDQDPQRVLSTLNVLVTPELLETGVECTASNDLGKNT
                     SILFLELVNLTTLTPDSNTTTGLSTSTASPHTRANSTSTERKLPEPESRGVVIVAVIV
                     CILVLAVLGAVLYFLYKKGKAAVQALREAGDHAAPVS"
                     /partial
     sig_peptide     8..91
                     /note="MUC18 glycoprotein signal peptide"
                     /codon_start=1
     mat_peptide     92..1816
                     /note="MUC18 glycoprotein"
                     /codon_start=1
     misc_feature    122..373
                     /note="immunoglobin like (V set) domain I"
     misc_feature    464..691
                     /note="immunoglobin like (V set) domain II"
     misc_feature    793..985
                     /note="immunoglobin like (C2 set) domain"
     misc_feature    1073..1246
                     /note="immunoglobin like (C2 set) domain"
     misc_feature    1334..1522
                     /note="immunoglobin like (C2 set) domain"
     misc_difference replace(1766,"g")
                     /citation=1
                     /note="g is deleted"
     misc_difference 1880^1881
                     /citation=1
                     /note="insert g"
     polyA_signal    3270..3275
BASE COUNT      786 a    914 c    922 g    668 t
ORIGIN
        1 gggaagcatg gggcttccca ggctggtctg cgccttcttg ctcgccgcct gctgctgctg
       61 tcctcgcgtc gcgggtgtgc ccggagaggc tgagcagcct gcgcctgagc tggtggaggt
      121 ggaagtgggc agcacagccc ttctgaagtg cggcctctcc cagtcccaag gcaacctcag
      181 ccatgtcgac tggttttctg tccacaagga gaagcggacg tcatcttccg tgtgcgccag
      241 ggccagggcc agagcgaacc tggggagtac gagcaagcgg ctcagcctcc aggacagagg
      301 ggctactctg gccctgactc aagtcacccc ccaagacgag cgcatcttct tgtgccaggg
      361 caagcgcctc ggtcccagga gtaccgcatc cagctccgcg tctacaaagc tccggatgcc
      421 aaacatccag gtcaaccccc tgggcatccc tgtgaacagt aaggagcctg aggaggtcgc
      481 tacctgtgta gggaggaacg ggtaccccat tcctcaagtc atctggtaca agaatggccg
      541 gcctctgaag gaggagaaga accgggtcca cattcagtcg tcccagactg tggagtcgag
      601 tggtttgtac accttgcaga gtattctgaa ggcacagctg gttaaagaag acaaagatgc
      661 ccagttttac tgtgagctca actaccggct gcccagtggg aaccacatga aggagtccag
      721 ggaagtcacc gtccctgttt tctacccgac agaaaaagtg tggctggaag tggagcccgt
      781 gggaatgctg aaggaagggg accgcgtgga aatcaggtgt ttggctgatg gcaaccctcc
      841 accacacttc agcatcagca agcagaaccc cagcaccagg gaggcagagg aagagacaac
      901 caacgacaac ggggtcctgg tgctggagcc tgcccggaag gaacacagtg ggcgctatga
      961 atgtcaggcc tggaacttgg acaccatgat atcgctgctg agtgaaccac aggaactact
     1021 ggtgaactat gtgtctgacg tccgagtgag tcccgcagcc cctgagagac aggaaggcag
     1081 cagcctcacc ctgacctgtg aggcagagag tagccaggac ctcgagttcc agtggctgag
     1141 agaagagaca gaccaggtgc tggaaagggg gcctgtgctt cagttgcatg acctgaaacg
     1201 ggaggcagga ggcggctatc gctgcgtggc gtctgtgccc agcatacccg gcctgaaccg
     1261 cacacagctg gtcaagctgg ccatttttgg ccccccttgg atggcattca aggagaggaa
     1321 ggtgtgggtg aaagagaata tggtgttgaa tctgtcttgt gaagcgtcag ggcacccccg
     1381 gcccaccatc tcctggaacg tcaacggcac ggcaagtgaa caagaccaag atccacagcg
     1441 agtcctgagc accctgaatg tcctcgtgac cccggagctg ttggagacag gtgttgaatg
     1501 cacggcctcc aacgacctgg gcaaaaacac cagcatcctc ttcctggagc tggtcaattt
     1561 aaccaccctc acaccagact ccaacacaac cactggcctc agcacttcca ctgccagtcc
     1621 tcataccaga gccaacagca cctccacaga gagaaagctg ccggagccgg agagccgggg
     1681 cgtggtcatc gtggctgtga ttgtgtgcat cctggtcctg gcggtgctgg gcgctgtcct
     1741 ctatttcctc tataagaagg gcaaggctgc cgtgcaggcg ctcagggaag caggagatca
     1801 cgctgccccc gtctcgtaag accgaacttg tagttgaagt taagtcagat aagctcccag
     1861 aagagatggg cctcctgcag gcagcagcgg tgacaagagg gctccgggag accagggaga
     1921 gaaatacatc gatctgaggc attagccccg aatcacttca gctcccttcc ctgcctggac
     1981 cattcccagc tccctgctca ctcttctctc agccaaagct caaagggact agagagaagc
     2041 ctcctgctcc cctcgcctgc acaccccctt tcagagggcc actgggttag gacctgagga
     2101 cctcacttgg ccctgcaagg cccgcttttc agggaccagt ccaccaccat ctcctccacg
     2161 ttgagtgaag ctcatcccaa gcaaggagcc ccagtctccc gagcgggtag gagagtttct
     2221 tgcagaacgt gttttttctt tacacacatt atgctgtaaa tacgctcgtc ctgccagcag
     2281 ctgagctggg tagcctctct gagctggttt cctgccccaa aggctggcat tccaccatcc
     2341 aggtgcacca ctgaagtgag gacacaccgg agccaggcgc ctgctcatgt tgaagtgcgc
     2401 tgttcacacc cgctccggag agcaccccag cagcatccag aagcagctgc agtgcaagct
     2461 tgcatgcctg cgtgttgctg caccaccctc ctgtctgcct cttcaaagtc tcctgtgaca
     2521 ttttttcttt ggtcagaggc caggaactgt gtcattcctt aaagatacgt gccggggcca
     2581 ggtgtggctc acgcctgtaa tcccagcact ttgggaggcc gaggcggcgg atcacaaagt
     2641 cagacgagac catcctggct aacacggtga aaccctgtct ctactaaaaa tacaaaaaaa
     2701 aattagctag gcgtagtggt tggcacctat agtcccagct actcggaagg ctgaagcagg
     2761 agaatggtat gaatccagga ggtggagctt gcagtgagcc gagaccgtgc cactgcactc
     2821 cagcctgggc aacacagcga gactccgtct cgaggaaaaa aaaaatcgtg ctcgtagcag
     2881 ctggctctgt ttcgagtcag gtgaattagc ctcaatcccc gtgttcactt gctcccatag
     2941 ccctcttgat ggatcacgta aaactgaaag gcagcgggga gcagacaaag atgaggtcta
     3001 cactgtcctt catggggatt aaagctatgg ttatattagc accaaacttc tacaaaccaa
     3061 gctcaggacc caaccctaga agggcccaaa tgagagaatg gtacttaggg atggcaaaac
     3121 gggcctggct agagcttcgg gtgtgtgtgt ctgtctgtgt atgcatacat atgtgtgtat
     3181 atatggtttt gtcaggtgtg taaatttgca aattgtttcc tttatatatg tatgtatata
     3241 tatatatgaa aatatatata tatatgaaaa ataaagctta attgtcccag
//