From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: H.sapiens COL2A1 mRNA for alpha1 (II) collagen
Date: 15 Aug 92 10:02:01 GMT
Distribution: bionet
Checksum: 24714     8

LOCUS       HUMCOL2A1R   3480 bp ss-RNA             PRI       15-AUG-1992
DEFINITION  H.sapiens COL2A1 mRNA for alpha1 (II) collagen
ACCESSION   X16711
KEYWORDS    collagen; collagen alpha 1 type II; extracellular protein;
            major structural protein; matrix protein.
SOURCE      Homo sapiens RNA.
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 3480)
  AUTHORS   Baldwin,C.T., Reginato,A.M., Smith,C., Jimenez,S.A. and
            Prockop,D.J.
  TITLE     Structure of cDNA clones coding for human type II collagen. The
            alpha 1 (II) chain is more similar to the alpha 1 (I) chain than
            two other alpha chains of fibrillar collagen
  JOURNAL   Biochem. J. 262, 521-528 (1989)
  STANDARD  full automatic
COMMENT     *source: stage=foetus (24 week); *source: tissue=cartilage;
            *source: cell_type=chondrocyte; *source: clone_library=59/60;
            *source: clone=AR113, PCR115, AR5960;
            From EMBL    entry HSCOL2A1R;  dated 31-JUL-1992.
FEATURES             Location/Qualifiers
     sig_peptide     1..69
     CDS             1..>3480
                     /gene="COL2A1"
                     /codon_start=1
                     /translation="MIRLGAPQSLVLLTLLVAAVLRCQGQDVRQPGPKGQKGEPGDIK
                     DIVGPKGPPGPQGPAGEQGPRGDRGDKGEKGAPGPRGRDGEPGTPGNPGPPGPPGPPG
                     PPGLGGNFAAQMAGGFDEKAGGAQLGVMQGPMGPMGPRGPPGPAGAPGPQGFQGNPGE
                     PGEPGVSGPMGPRGPPGPPGKPGDDGEAGKPGKAGERGPPGPQGARGFPGTPGLPGVK
                     GHRGYPGLDGAKGEAGAPGVKGESGSPGENGSPGPMGPRGLPGERGRTGPAGAAGARG
                     NDGQPGPAGPPGPVGPAGGPGFPGAPGAKGEAGPTGARGPEGAQGPRGEPGTPGSPGP
                     AGASGNPGTDGIPGAKGSAGAPGIAGAPGFPGPRGPPGPQGATGPLGPKGQTGEPGIA
                     GFKGEQGPKGEPGPAGPQGAPGPAGEEGKRGARGEPGGVGPIGPPGERGAPGNRGFPG
                     QDGLAGPKGAPGERGPSGLAGPKGANGDPGRPGEPGLPGARGLTGRPGDAGPQGKVGP
                     SGAPGEDGRPGPPGPQGARGQPGVMGFPGPKGANGEPGKAGEKGLPGAPGLRGLPGKD
                     GETGAAGPPGPAGPAGERGEQGAPGPSGFQGLPGPPGPPGEGGKPGDQGVPGEAGAPG
                     LVGPRGERGFPGERGSPGAQGLQGPRGLPGTPGTDGPKGASGPAGPPGAQGPPGLQGM
                     PGERGAAGIAGPKGDRGDVGEKGPEGAPGKDGGRGLTGPIGPPGPAGANGEKGEVGPP
                     GPAGSAGARGAPGERGETGPPGPAGFAGPPGADGQPGAKGEQGEAGQKGDAGAPGPQG
                     PSGAPGPQGPTGVTGPKGARGAQGPPGATGFPGAAGRVGPPGSNGNPGPPGPPGPSGK
                     DGPKGARGDSGPPGRAGEPGLQGPAGPPGEKGEPGDDGPSGAEGPPGPQGLAGQRGIV
                     GLPGQRGERGFPGLPGPSGEPGKQGAPGASGDRGPPGPVGPPGLTGPAGEPGRQGSPG
                     ADGPPGRDGAAGVKGDRGETGAVGAPGTPGPPGSPGPAGPTGKQGDRGEAGAQGPMGP
                     SGPAGARGIQGPQGPRGDKGEAGEPGERGLKGHRGFTGLQGLPGPPGPSGDQGASGPA
                     GPSGPRGPPGPVGPSGKDGANGIPGPIGPPGPRGRSGETGPAGPPGNPGPPGPPGPPG
                     PGIDMSAFAGLGPR"
     misc_feature    70..336
                     /note="N-propeptide"
     mat_peptide     337..3480
                     /product="collagen"
     misc_feature    337..393
                     /note="N-telopeptide"
     misc_feature    394..3435
                     /note="triple helical domain"
     misc_feature    3436..3480
                     /note="C-telopeptide"
BASE COUNT      582 a   1055 c   1214 g    629 t
ORIGIN
        1 atgattcgcc tcggggctcc ccagtcgctg gtgctgctga cgctgctcgt cgccgctgtc
       61 cttcggtgtc agggccagga tgtccggcaa ccaggaccaa agggacagaa aggagaacct
      121 ggagacatca aggatattgt aggacccaaa ggacctcctg ggcctcaggg acctgcaggg
      181 gaacaaggac ccagagggga tcgtggtgac aaaggtgaaa aaggtgcccc tggacctcgt
      241 ggcagagatg gagaacctgg gacccctgga aatcctggcc cccctggtcc tcccggcccc
      301 cctggtcccc ctggtcttgg tggaaacttt gctgcccaga tggctggagg atttgatgaa
      361 aaggctggtg gcgcccagtt gggagtaatg caaggaccaa tgggccccat gggacctcga
      421 ggacctccag gccctgcagg tgctcctggc cctcaaggat ttcaaggcaa tcctggtgaa
      481 cctggtgaac ctggtgtctc tggtcccatg ggtccccgtg gtcctcctgg tccccctgga
      541 aagcctggtg atgatggtga agctggaaaa cctggaaaag ctggtgaaag gggtccgcct
      601 ggtcctcagg gtgctcgtgg tttcccagga accccaggcc ttcctggtgt caaaggtcac
      661 agaggttatc caggcctgga cggtgctaag ggagaggcgg gtgctcctgg tgtgaagggt
      721 gagagtggtt ccccgggtga gaacggatct ccgggcccaa tgggtcctcg tggcctgcct
      781 ggtgaaagag gacggactgg ccctgctggc gctgcgggtg cccgaggcaa cgatggtcag
      841 ccaggccccg caggtcctcc gggtcctgtc ggtcctgctg gtggtcctgg cttccctggt
      901 gctcctggag ccaagggtga agccggcccc actggtgccc gtggtcctga aggtgctcaa
      961 ggtcctcgcg gtgaacctgg tactcctggg tcccctgggc ctgctggtgc ctccggtaac
     1021 cctggaacag atggaattcc tggagccaaa ggatctgctg gtgctcctgg cattgctggt
     1081 gctcctggct tccctgggcc acggggtcct cctggccctc aaggtgcaac tggtcctctg
     1141 ggcccgaaag gtcagacggg tgaacctggt attgctggct tcaaaggtga acaaggcccc
     1201 aagggagaac ctggccctgc tggcccccag ggagcccctg gacccgctgg tgaagaaggc
     1261 aagagaggtg cccgtggaga gcctggtggc gttgggccca tcggtccccc tggagaaaga
     1321 ggtgctcccg gaaaccgcgg tttcccaggt caagatggtc tggcaggtcc caagggagcc
     1381 cctggagagc gagggcccag tggtcttgct ggccccaagg gagccaacgg tgaccctggc
     1441 cgtcctggag aacctggcct tcctggagcc cggggtctca ctggccgccc tggtgatgct
     1501 ggtcctcaag gcaaagttgg cccttctgga gcccctggtg aagatggtcg tcctggacct
     1561 ccaggtcctc agggggctcg tgggcagcct ggtgtcatgg gtttccctgg ccccaaaggt
     1621 gccaacggtg agcctggcaa agctggtgag aagggactgc ctggtgctcc tggtctgagg
     1681 ggtcttcctg gcaaagatgg tgagacaggt gctgcaggac cccctggccc tgctggacct
     1741 gctggtgaac gaggcgagca gggtgctcct gggccatctg ggttccaggg acttcctggc
     1801 cctcctggtc ccccaggtga aggtggaaaa ccaggtgacc agggtgttcc cggtgaagct
     1861 ggagcccctg gcctcgtggg tcccaggggt gaacgaggtt tcccaggtga acgtggctct
     1921 cccggtgccc agggcctcca gggtccccgt ggcctccccg gcactcctgg cactgatggt
     1981 cccaaaggtg catctggccc agcaggcccc cctggcgcac agggccctcc aggtcttcag
     2041 ggaatgcctg gcgagagggg agcagctggt atcgctgggc ccaaaggcga caggggtgac
     2101 gttggtgaga aaggccctga gggagcccct ggaaaggatg gtggacgagg cctgacaggt
     2161 cccattggcc cccctggccc agctggtgct aacggcgaga agggagaagt tggacctcct
     2221 ggtcctgcag gaagtgctgg tgctcgtggc gctccgggtg aacgtggaga gactggcccc
     2281 cccggaccag cgggatttgc tgggcctcct ggtgctgatg gccagcctgg ggccaagggt
     2341 gagcaaggag aggccggcca gaaaggcgat gctggtgccc ctggtcctca gggcccctct
     2401 ggagcacctg ggcctcaggg tcctactgga gtgactggtc ctaaaggagc ccgaggtgcc
     2461 caaggccccc cgggagccac tggattccct ggagctgctg gccgcgttgg acccccaggc
     2521 tccaatggca accctggacc ccctggtccc cctggtcctt ctggaaaaga tggtcccaaa
     2581 ggtgctcgag gagacagcgg cccccctggc cgagctggtg aacccggcct ccaaggtcct
     2641 gctggacccc ctggcgagaa gggagagcct ggagatgacg gtccctctgg tgccgaaggt
     2701 ccaccaggtc cccagggtct ggctggtcag agaggcatcg tcggtctgcc tgggcaacgt
     2761 ggtgagagag gattccctgg cttgcctggc ccatcgggtg agcccggcaa gcagggtgct
     2821 cctggagcat ctggagacag aggtcctcct ggccccgtgg gtcctcctgg cctgacgggt
     2881 cctgcaggtg aacccggacg acagggaagc cccggtgctg atggcccccc tggcagagat
     2941 ggcgctgctg gagtcaaggg tgatcgtggt gagactggtg ctgtgggagc tcctggaacc
     3001 cctgggcccc ctggctcccc tggccccgct ggtccaactg gcaagcaagg agacagagga
     3061 gaagctggtg cacaaggccc catgggaccc tcaggaccag ctggagcccg gggaatccag
     3121 ggtcctcaag gccccagagg tgacaaagga gaggctggag agcctggcga gagaggcctg
     3181 aagggacacc gtggcttcac tggtctgcag ggtctgcccg gccctcctgg tccttctgga
     3241 gaccaaggtg cttctggtcc tgctggtcct tctggcccta gaggtcctcc tggccccgtc
     3301 ggtccctctg gcaaagatgg tgctaatgga atccctggcc ccattgggcc tcctggtccc
     3361 cgtggacgat caggcgaaac cggccctgct ggtcctcctg gaaatcctgg gccccctggt
     3421 cctccaggtc cccctggccc tggcatcgac atgtccgcct ttgctggctt aggcccgaga
//