From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: X.laevis mRNA encoding apical protein.
Date: 12 Sep 92 10:04:19 GMT
Distribution: bionet
Checksum: 60104    10

LOCUS       XELAPICAL    4900 bp ss-RNA             VRT       12-SEP-1992
DEFINITION  X.laevis mRNA encoding apical protein.
ACCESSION   Z14997
KEYWORDS    apical complex protein.
SOURCE      Xenopus laevis RNA.
  ORGANISM  Xenopus laevis
            Eukaryota; Animalia; Chordata; Vertebrata; Amphibia; Lissamphibia;
            Anura; Archeobatrachia; Pipoidea; Pipidae; Xenopodinae.
REFERENCE   1  (bases 1 to 4900)
  AUTHORS   Staub,O., Verrey,F., Kleyman,T., Benos,D., Rossier,B. and
            Kraehenbuhl,J.P.
  TITLE     Primary structure of an apical protein from Xenopus laevis that
            participates in amiloride-sensitive sodium channel activity
  JOURNAL   Unpublished (1992)
  STANDARD  full staff_review
REFERENCE   2  (bases 1 to 4900)
  AUTHORS   Kraehenbuhl,J.P.
  JOURNAL   Unpublished (1992)
  STANDARD  full staff_review
COMMENT     *source: tissue=Ovary; *source: cell_line=A6; *source: clone=Apx;
            *source: is_macronuclear=N; *source: is_proviral=N; *source:
            is_germline=N;
            From EMBL    entry XLAPICAL;  dated 26-AUG-1992.
FEATURES             Location/Qualifiers
     CDS             226..4488
                     /function="Amiloride-sensitive sodium channel"
                     /product="apical protein"
                     /evidence=EXPERIMENTAL
                     /codon_start=1
                     /translation="MSAFGNTIERWNIKSTGVIAGLGHSERISPVRSMTTLVDSAYSS
                     FSGSSYVPEYQNSFQHDGCHYNDEQLSYMDSEYVRAIYNPSLLDKDGVYNDIVSEHGS
                     SKVALSGRSSSSLCSDNTTSVHRTSPAKLDNYVTNLDSEKNIYGDPINMKHKQNRPNH
                     KAYGLQRNSPTGINSLQEKENQLYNPSNFMEIKDNYFGRSLDVLQADGDIMTQDSYTQ
                     NALYFPQNQPDQYRNTQYPGANRMSKEQFKVNDVQKSNEENTERDGPYLTKDGQFVQG
                     QYASDVRTSFKNIRRSLKKSASGKIVAHDSQGSCWIMKPGKDTPSFNSEGTITDMDYD
                     NREQWDIRKSRLSTRASQSLYYESNEDVSGPPLKAMNSKNEVDQTLSFQKDATVKSIP
                     LLSQQLQQEKCKSHPLSDLNCEKITKASTPMLYHLAGGRHSAFIAPVHNTNPAQQEKL
                     KLESKTLERMNNISVLQLSEPRPDNHKLPKNKSLTQLADLHDSVEGGNSGNLNSSAEE
                     SLMNDYIEKLKVAQKKVLRETSFKRKDLQMSLPCRFKLNPPKRPTIDHFRSYSSSSAN
                     EESAYLQTKNSADSSYKKDDTEKVAVTRIGGRKRITKEQKKLCYSEPEKLDHLGIQKS
                     NFAWKEEPTFANRREMSDSDISANRIKYLESKERTNSSSNLSKTELKQIQHNALVQYM
                     ERKTNQRPNSNPQVQMERTSLGLPNYNEWSIYSSETSSSDASQKYLRRRSAGASSSYD
                     ATVTWNDRFGKTSPLGRSAAEKTAGVQRKTFSDQRTLDGSQEHLEGSSPSLSQKTSKS
                     THNEQVSYVNMEFLPSSHSKNHMYNDRLTVPGDGTSAESGRMFVSKSRGKSMEEIGTT
                     DIVKLAELSHSSDQLYHIKGPVISSRLENTRTTAASHQDRLLASTQIETGNLPRQTHQ
                     ESVVGPCRSDLANLGQEAHSWPLRASDVSPGTDNPCSSSPSAEVQPGAPEPLHCLQTE
                     DEVFTPASTARNEEPNSTAFSYLLSTGKPVSQGEATALSFTFLPEQDRLEHPIVSETT
                     PSSESDENVSDAAAEKETTTTQLPETSNVNKPLGFTVDNQEVEGDGEPMQPEFIDSSK
                     QLELSSLPSSQVNIMQTAEPYLGDKNIGNEQKTEDLEQKSKNPEEDDLPKVKLKSPED
                     EILEELVKEIVAKDKSLLNCLQPVSVRESAMDLMKSLFPMDVTAAEKSRTRGLLGKDK
                     GETLKKNNSDLESSSKLPSKITGMLQKRPDGESLDDITLKKMELLSKIGSKLEDLCEQ
                     REFLLSDISKNTTNGNNMQTMVKELCKPNEFERYMMFIGDLEKVVSLLFSLSTRLTRV
                     ENSLSKVDENTDAEEMQSLKERHNLLSSQREDAKDLKANLDRREQVVTGILVKYLNEE
                     QLQDYKHFVRLKTSLLIEQKNLEEKIKVYEEQFESIHNSLPP"
                     /citation=[1]
                     /citation=[2]
BASE COUNT     1652 a    997 c   1042 g   1209 t
ORIGIN
        1 ggtgcggatt gtgtgtttgc tgcgagacgc tgtgtattcg ctacaccgac gcagaactgc
       61 ggcacttata gaactacagg aggggcattg ctttatcccc tgctgagtga cgacagaaat
      121 gcaggtgttt gtttttgagc tacgcagaag aaaatgccag ttctccataa acatcttgaa
      181 gaattctaaa taaatcaatt cagtttcaaa gggcctcagt cagtaatgtc agcttttggc
      241 aatacaattg agagatggaa cataaaaagt acaggagtca ttgctggtct gggacattct
      301 gagagaatat cgcctgtaag atccatgacc acattagtgg attcagccta tagctcattt
      361 tctggcagtt catatgttcc agaataccaa aactcatttc agcatgacgg ctgtcattat
      421 aatgatgaac agctttctta catggactct gaatatgtaa gagccatcta taatcctagt
      481 ttattagaca aggatggtgt atataatgat atagtgtcag aacatggaag ctcaaaagta
      541 gcactgtctg gaagatctag cagctcttta tgttctgaca acacaacatc tgttcatcga
      601 acttcaccag caaaactgga taattatgtt actaacctgg atagtgagaa aaatatttat
      661 ggggatccaa taaatatgaa gcataagcag aatcgcccta accacaaagc ttatggcctc
      721 caacgaaata gtcccacagg aataaacagt ttacaggaaa aagaaaacca attgtataat
      781 ccatcaaatt ttatggaaat aaaagataat tattttggta gatctttaga tgttcttcaa
      841 gcagatggag acattatgac acaagattca tatacccaga atgccctata ttttcctcag
      901 aatcagccag atcagtacag aaacacacaa tatccaggtg caaatagaat gagtaaagaa
      961 cagttcaagg taaacgatgt acaaaaaagc aatgaagaaa acacagaaag agatggacca
     1021 tatctgacaa aagatggtca gtttgttcaa ggccaatatg catcagatgt aaggacaagt
     1081 tttaaaaaca taagacgttc ccttaaaaag tctgcgtccg gaaagattgt tgctcatgac
     1141 agtcaaggta gctgctggat tatgaagcca ggtaaagaca ctccttcttt taattctgaa
     1201 gggaccatta ctgacatgga ctatgataac cgtgagcagt gggatatcag gaagtccagg
     1261 cttagtacaa gggcaagcca aagtttatat tatgagtcta atgaggatgt ctccggccct
     1321 cccttaaaag ctatgaattc caagaatgaa gttgaccaga cactcagttt ccaaaaagat
     1381 gcaactgtaa agtctattcc tttgttatct cagcagcttc aacaggaaaa atgtaaaagc
     1441 caccccctgt ctgatttaaa ctgtgagaag attaccaagg catcaacacc tatgctgtat
     1501 catcttgctg gagggagaca tagtgcattt atagcccctg tacacaatac aaatccagct
     1561 caacaagaaa aactaaagct tgagtcaaag actttggaaa gaatgaataa catttctgta
     1621 ctacagctat cagagccaag accagataac cataaactcc caaagaataa aagcctgaca
     1681 caacttgctg atctccatga ctctgtagag ggtgggaatt ctggcaattt aaattcttca
     1741 gcagaagagt ccttaatgaa tgactacatt gagaaactga aggttgcgca gaagaaagta
     1801 ctgagggaaa cctcgtttaa aagaaaggac ttgcagatga gtttgccttg cagattcaag
     1861 ctaaatccac ccaaaaggcc caccattgat cattttcgat cctattcttc atccagtgca
     1921 aatgaggaga gtgcgtatct ccagacaaaa aactctgctg acagcagtta caaaaaagat
     1981 gacactgaaa aggttgcagt tactcgaata ggaggacgga aaaggattac aaaggaacaa
     2041 aagaagctgt gttattcaga accagagaaa ttagatcacc ttgggattca aaaatctaac
     2101 tttgcatgga aggaggaacc cacttttgca aataggaggg agatgagtga ttctgatatt
     2161 agtgcaaaca ggataaagta tctggagagc aaagagcgaa ccaactcatc ttcaaatctt
     2221 tctaagacag aactcaaaca aatccagcat aatgcactgg ttcaatatat ggagagaaaa
     2281 acaaaccaaa ggcccaacag caatccacaa gttcagatgg aaaggacgtc gttgggattg
     2341 ccaaattata atgaatggag tatctactcc tctgaaacca gttcaagtga tgcttcccaa
     2401 aagtacctaa ggaggagatc agctggagca tcatcttcat atgatgccac agtaacgtgg
     2461 aatgatagat ttggaaagac ttcacctctt ggacgttctg cagcagaaaa aactgctggc
     2521 gtacaacgca agactttctc tgatcagcgc actttagatg gcagccaaga acatctggaa
     2581 ggttcttccc catcactcag tcaaaagacc tcaaagtcaa ctcataatga acaggtctct
     2641 tatgtgaata tggaatttct tccctcgtca cattccaaga atcacatgta taatgaccgg
     2701 ctgactgtac ccggagacgg caccagtgca gagtctggaa gaatgtttgt ttctaaaagc
     2761 agaggaaagt ccatggaaga aatagggact acagacattg taaagcttgc agagctgagc
     2821 catagctccg atcaacttta ccacattaaa gggccggtga tatcttcaag gctggaaaac
     2881 acaagaacta cagctgcatc ccatcaagac agactcttgg ccagcactca gattgaaact
     2941 ggaaatctgc ccagacaaac acaccaagaa agtgttgtgg ggccctgcag atcagattta
     3001 gcaaatttgg gacaggaggc acattcctgg cccttgagag ctagtgatgt atccccaggc
     3061 acagataatc cctgctcatc ttctccttca gcagaggttc agcccggggc tccagagcca
     3121 ttgcattgct tgcaaacaga agatgaggtc tttacgccag ccagcactgc cagaaatgaa
     3181 gagcccaact ccactgcctt tagttatttg ctcagtactg gaaaacctgt gtcccaaggg
     3241 gaagctactg cattatcctt tactttctta cctgagcagg atcgtctaga acacccaatt
     3301 gtcagtgaga ctacaccaag ctctgagtct gatgaaaatg tttcggacgc tgctgctgag
     3361 aaagagacga caacaacaca acttcctgag acttcaaacg tcaacaagcc acttggcttt
     3421 actgttgata atcaagaagt tgaaggggat ggagaaccta tgcaaccaga gtttatagac
     3481 agctcaaagc agttagagtt gagttccctt ccttccagcc aggttaacat aatgcagact
     3541 gcagagcctt atcttggtga taagaatatc ggaaatgagc aaaaaacgga agaccttgag
     3601 cagaaaagta agaatccaga agaagacgat ttacccaagg tcaagttaaa atctccagaa
     3661 gatgaaatac tggaggaact ggttaaagag atagttgcca aggataaatc tctgctgaat
     3721 tgcctccagc ctgtttcagt cagagaatct gctatggatc tcatgaaaag cctcttccct
     3781 atggacgtca ctgcagcgga aaaatctaga actagaggac ttcttggaaa agacaagggg
     3841 gaaacattaa agaaaaacaa ctcggacctt gaaagttctt ctaaacttcc ttcaaaaata
     3901 acagggatgc ttcagaagag gccagatggg gaaagtctag atgacatcac tttaaagaaa
     3961 atggaactcc tttccaaaat tggttccaag ctagaagacc tttgtgagca gagagaattc
     4021 cttttgtctg atataagtaa gaacacgacc aacggcaata atatgcagac aatggtaaaa
     4081 gagctgtgta agcccaatga gtttgagcgc tacatgatgt tcattgggga tctggagaaa
     4141 gtggtgagcc tgctgttttc cctgtccaca aggctgactc gggtcgaaaa ctctttaagc
     4201 aaagttgatg aaaatacaga tgcagaagag atgcaatccc taaaggaacg tcacaatctc
     4261 ctgtccagtc aaagagaaga tgccaaagac ctaaaggcaa acttggaccg cagggaacaa
     4321 gttgtcacag gaattcttgt aaaatacctg aatgaggagc agctgcagga ctacaagcac
     4381 tttgtgagac tgaagacctc actcctgatt gagcaaaaga accttgagga gaagataaaa
     4441 gtgtatgagg agcagtttga gagcatccac aatagcctcc caccctaatc atcagccaac
     4501 acaagatcag ctcttgtttt tctcaccgtc cacccgctac aaatgaaaat tctctctggg
     4561 tttcatgtca ttgaaatttc tctagtgttg attaaactgc tgctaaaact taacacggtt
     4621 aattctcgtt cttccgtgtt tcgacgggac ttgctaaagg ccactctgac agtgttaatt
     4681 cggctctttg gcaagaaaga gacggggggt gaatactaca caagctggac agtggttagc
     4741 tatgattgtg tgccttttta ttttgcataa gttatcaatg ttttgtcttt ttttcaatgt
     4801 tattgacgac taacagcgtg tccttgtaat ttggattcct gacaaaatga tttaaataaa
     4861 aatgtttgtt tctgcccagg aaaaaaaaaa aaaaaaaaaa
//