From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: D.pseudoobscura Adh gene for alcohol dehydrogenase
Date: 28 Dec 92 21:26:27 GMT
Distribution: bionet
Checksum: 18303 8

LOCUS       DROADHGN11   3472 bp ds-DNA             INV       01-DEC-1992
DEFINITION  D.pseudoobscura Adh gene for alcohol dehydrogenase
ACCESSION   X62191
KEYWORDS    ADH gene; alcohol dehydrogenase.
SOURCE      Drosophila pseudoobscura DNA.
  ORGANISM  Drosophila pseudoobscura
            Eukaryota; Animalia; Metazoa; Arthropoda; Uniramia; Insecta;
            Pterygota; Neoptera; Holometabola; Diptera; Brachycera;
            Cyclorrhapha; Schizophora; Drosophiloidea; Drosophilidae.
REFERENCE   1  (bases 1 to 3472)
  AUTHORS   Schaeffer,S.W. and Miller,E.L.
  TITLE     Estimates of gene flow in Drosophila pseudoobscura determined from
            nucleotide sequence analysis of the alcohol dehydrogenase region
  JOURNAL   Genetics 132, 471-480 (1992)
  STANDARD  full automatic
COMMENT     *source: strain=GB103; *source: isolate=Gundlach Bundschu Winery,
            Sonoma Valley, *source: California; *source: chromosome=IV section
            88;
               From EMBL    entry DPADHGN11;  dated 16-OCT-1992.
FEATURES             Location/Qualifiers
     mRNA            join(40..119,925..1030,1094..1498,1559..1974)
                     /note="adult mRNA (Adh)"
     mRNA            join(892..1030,1094..1498,1559..1974)
                     /note="larval mRNA (Adh)"
     promoter        8..14
                     /note="adult promoter (Adh)"
     prim_transcript 40..1974
                     /note="adult primary transcript (Adh)"
     exon            40..119
                     /note="adult exon 1a"
     intron          120..924
                     /note="adult intron"
     promoter        856..863
                     /note="larval promoter (Adh)"
     prim_transcript 892..1974
                     /note="larval primary transcript (Adh)"
     exon            892..1030
                     /note="larval exon 1"
     exon            925..1030
                     /note="adult exon 1b"
     intron          1031..1093
                     /number=1
     exon            1094..1498
                     /number=2
     intron          1499..1558
                     /number=2
     exon            1559..1974
                     /number=3
     polyA_signal    1951..1956
     polyA_site      1974
     CDS             join(938..1030,1094..1498,1559..1825)
                     /EC_number="1.1.1.1"
                     /gene="Adh"
                     /product="alcoholdehydrogenase"
                     /codon_start=1
                     /translation="MSLTNKNVVFVAGLGGIGLDTSRELVKRNLKNLVILDRIDNPAA
                     IAELKAINPKVTITFYPYDVTVPVAETTKLLKTIFAQVKTIDVLINGAGILDDHQIER
                     TIAVNYTGLVNTTTAILDFWDKRKGGPGGIICNIGSVTGFNAIYQVPVYSGSKAAVVN
                     FTSSLAKLAPITGVTAYTVNPGITKTTLVHKFNSWLDVEPRVAEKLLEHPTQTSQQCA
                     ENFVKAIELNKNGAIWKLDLGTLEPITWTQHWDSGI"
BASE COUNT      965 a    793 c    798 g    916 t
ORIGIN      
        1 cgcagtgtat ttaaggcact gcacatgtcg aggaccacga ttattgtcta cagtgcagtc
       61 agtagttgtc aggtgcagca ggcggaccag catttcttta aatctactca atcgatcaag
      121 taagtaactg tgtaatccaa gtacagtcgg cccatcccac tcggtcactc ggttctgccg
      181 tagctcaaga aatttcttga tagcgcagtt ccagttgtag aacgtcgctt aaaaaaacat
      241 ctcgttggag cttgggttga aaaacgttcg agttgggcgt agaaaatttc aaatataaaa
      301 tatcaaaaaa gagaaaatat cttcccaaag cagctgtcca ttttttgtta aagtgataga
      361 tcaaaaattc gtatgacgta tttgccaagt gctctttatg caaataattt cctcaattct
      421 aattctacaa gaagcacttt tggctcttgt ccagaaaaaa caatttgttt acacttgtcg
      481 gttgataaca gaggcggacc gacggctaag taacataaca gtcgcctgtt aagtaatgcc
      541 ctcactctct gttagatggc cgcgcgtgtc gactccgata gaccagcaga cacgcagtag
      601 ttttcctcaa gaacctgaac tctaaacata gacataattt acgatacctt gagactcaaa
      661 taaaaataca agtatgagtg gtattctatt cctgattaga ctcgcacaca cacacgtacg
      721 agtattaaca taaatttgcc ggcaaataac caaaacaaaa cgaacgcaaa cgatccgaac
      781 acgtaatgcg agagataaga aacagaaaag ctcgacgtga aagaagcttc tggcggaagc
      841 tcttgccgag ctccctataa atacgagaca gaaaaccaga ataaatataa taacaaactg
      901 ttgaattgtc ccatcgattc ccagatcaca agacagaatg tctctcacca acaagaacgt
      961 cgttttcgtg gccggtctgg gcggcattgg cctggacacc agccgggagt tggtcaagcg
     1021 taatctgaag gtaagagtga acgaattcca tggagtctat ggaatcctaa atttaaaaat
     1081 ttattttttt tagaacctgg tcatcctgga tcgcattgac aatccggctg ccattgccga
     1141 actgaaggca atcaatccca aggtgaccat caccttctat ccctacgatg tgactgtgcc
     1201 cgtcgctgag accaccaagc tcctgaagac catctttgcc caggtgaaga caatcgatgt
     1261 cctgatcaac ggtgctggca tcctggacga tcatcagatc gagcgcacca ttgccgttaa
     1321 ctacacgggc ctggtcaaca ccaccacagc cattctggac ttctgggaca agcgcaaggg
     1381 cggcccaggc ggcatcattt gcaacattgg ctccgtcacc ggtttcaatg ccatctacca
     1441 ggtgcccgtt tactccggct ccaaggcggc ggtggttaac tttacctcct ccctggcggt
     1501 aagtaatcca acctttttcc tactccccaa aactaaatat ttctttgaaa tcccatagaa
     1561 actggctccc attactggtg tcactgctta cactgtcaat cctggcatca ctaagaccac
     1621 tctggtccac aaattcaact cgtggctgga tgtggagccc cgtgtggcag agaagctgct
     1681 cgagcatccc acccagacct ctcagcagtg cgccgagaac tttgtgaagg ccatcgagct
     1741 gaacaagaac ggtgccatct ggaagttgga tctgggcacc ttggagccca tcacatggac
     1801 ccagcactgg gactctggca tctaaaccat cccagagact ctatgggaca tggcgtttta
     1861 gcttttagtt tctttttttc cactcaattg ttacgtatat acatacacat atggcaataa
     1921 ggctgatttg aaccccgttt tgaatatgat aatacaaaat tatatttgag aaatttcaac
     1981 aaaatcgata agagctatag ctctctttta attaaaaaaa actagataaa aagagcaatg
     2041 gtcagtggtg gtcagtggtc gttgtggcct ctatcgattt ccacacaaaa actttactgt
     2101 ttagtaatag aaaagaactt gagaaaggca gccaaaagaa tgtacgatct gacgggtaag
     2161 catgtctgct atgtggctga ctgcggtggc attgcactgg agaccagcaa ggttctcatg
     2221 accaagaata tagcggtgag tggtagagag gtggtgtaga aagtggagag tggttctggt
     2281 tcttaaacaa gtctctagtc tcttggctgc tgctggtagc tgtcgaaact aattaagtta
     2341 atgacttttt catgttttgt ttttgtccgc tgacttttgc tgttcaaagc tacaattaag
     2401 tttatgtttt gagtttgatc tacttttgct taagggaagt actttcgatt acggattgct
     2461 ggcatataaa aatagatacc aaggaatgtg catcatatca tattgattct atcttgattc
     2521 tttgcctaga aactggccat ccttcagagc gtggaaaacc ccccggccat cgctcagtta
     2581 caatcgatta agcacagcac acagatcttc ttctggacct tcgatgtgac catggcccgc
     2641 gaggagatga agaagtactt cgatgaggtc atggtccaga tggactacat cgatgtactg
     2701 atcaatgggg ccaccctgtg cgatgagcgg aacattgatg ccaccatcaa tacgaatctg
     2761 accggcatga tgaacaccgt ggccactgtg ctgccctata tggaccgtaa gatgggcggt
     2821 tcgggtggac tgattgtgaa tgtcacctcc gtcataggac tggatccatc gccagtcttt
     2881 tgtgcgtaca gtgcctcaaa gtttggtgtg attgggttca cccgaagtct agcggtgagt
     2941 gggcgagatc ttaacttgaa aatcctctct aatgtcttgc attctttcgc aggatcccct
     3001 gtattacacc caaaatggtg tggctgtcat ggccgtctgt tgtggtccca ccaaagtgtt
     3061 tgtcgatcgg gaactgactg ccttcctgga atacggccag tcctttgccg atcgcctgcg
     3121 tcgtgccccc tgccaatcga cggccgtctg cggccagaat atagtaaatg ccatcgagag
     3181 atcggagaac ggacagatct ggattgccga caagggcggc ctggagtcgg tggccctcca
     3241 ctggtattgg catatggccg atcagtttgt gaactatatg cagagcactg acgatgagga
     3301 tcaggagttt tttttgggcc agcgatgagg catgccttga tccacggaat aaaacatcaa
     3361 atgtgtcaca aaaagtaggc aacttgtttg tgggtctgga aatagatttg aaatattttg
     3421 aggtcatatg aataatgaca taaatgaggg tgcctgaaag tatgccaagt tt
//