From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: morphogene cluster: spoVE=mra homolog required for spore cortex
Date: 6 Jan 93 06:59:59 GMT
Distribution: bionet
Checksum: 11462 5

LOCUS       M257759S1    1200 bp    DNA             BCT       27-DEC-1992
DEFINITION  morphogene cluster: spoVE=mra homolog required for spore cortex
            formation...orf2 3' of murG [Bacillus subtilis, Genomic, 5 genes,
            1200 nt, segment 1 of 2] 
ACCESSION   S47095
KEYWORDS    .
SEGMENT     1 of 2
SOURCE      Bacillus subtilis
  ORGANISM  Bacillus subtilis
            Unclassified.
REFERENCE   1  (bases 1 to 1200)
  AUTHORS   Henriques,A.O., de Lencastre,H. and Piggot,P.J.
  TITLE     A Bacillus subtilis morphogene cluster that includes spoVE is
            homologous to the mra region of Escherichia coli.
  JOURNAL   Biochimie 74, 735-748 (1992)
  STANDARD  full automatic
COMMENT     This entry [NCBI gibbsq 116424] was created by the journal scanning
            component of NCBI/GenBank at the National Library of Medicine.
            This sequence comes from Fig. 2.
            Region: morphogene cluster.  
FEATURES             Location/Qualifiers
     CDS             3..1112
                     /partial
                     /note="MurD;  peptidoglycan biosynthesis;  For the protein
                     sequence (NCBI gibbsq 116425):  Method: conceptual
                     translation supplied by author.  This sequence comes from
                     Fig. 2."
                     /gene="murD"
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate
                     ligase"
                     /codon_start=1
                     /translation="IPYENIMVQEAEKRGIPVWTEIELAYYLTSAKFIGITGSNGKTT
                     TTTLIYEMLKADSQKALIAGNIGTVASEVAYHADGDEWIVTELSSFQLMGTHAFRPEI
                     SLILNVFDAHLDYHHTRENYEKAKQKVYLHQTASDKAIVNQDDETVVRLAEAGKAEIV
                     PFSVSKTLEQGAYVKDSMIMFNGEAILPLEEVVLPGAHNLENILAAIAVVKTAGASNE
                     AVKKVLTSFTGVKHRLQYVTTVNGRKFYNDSKATNILATSKRLSAFDKPVILLAGGLD
                     RGNGFDDLKPYMKHVKAVLTFGQTAPKLEKLGNELGIQHVKRVDNVEQAVSAAFALSN
                     EGDVILLSPACASWDQFKTFEERGDMFIDAVHMLK"
     CDS             1176..1199
                     /partial
                     /note="TTG start codon.  mra homolog required for spore
                     cortex formation;  For the protein sequence (NCBI gibbsq
                     116434):  Method: conceptual translation supplied by
                     author.  This sequence comes from Fig. 2.
                     Author-given protein sequence differs from the conceptual
                     translation for reasons explained in citation"
                     /gene="spoVE"
                     /product="SpoVE"
                     /codon_start=1
                     /translation="MTTKKTSPDL"
BASE COUNT      356 a    258 c    292 g    294 t
ORIGIN      
        1 gaattcctta tgaaaatatc atggttcagg aagcagagaa acgaggtatt ccggtttgga
       61 cggaaattga gctcgcttac tacctgacca gcgccaagtt tatcgggatt accggatcga
      121 acggtaaaac gaccacgaca acgctaattt acgaaatgct caaggctgac tcgcaaaaag
      181 cactgattgc cggaaacatc ggcacagtcg caagtgaggt tgcctatcat gctgatggag
      241 atgaatggat cgttaccgaa ctgtcttcgt ttcagctgat gggaacacac gctttcagac
      301 cggaaatcag cctgatttta aatgtatttg acgcccatct ggattatcac catacgcgcg
      361 aaaactatga aaaagcgaaa cagaaagtgt accttcatca gactgcttca gataaagcta
      421 ttgtcaacca ggatgatgaa accgtcgtgc gtcttgctga agccggaaaa gccgaaatcg
      481 tcccattttc agtcagcaaa acgcttgaac agggcgcata tgtaaaagac agtatgatta
      541 tgtttaacgg cgaagccatt cttcctctgg aagaggttgt actgcccggt gctcataacc
      601 tggagaacat tttagcagct attgcagttg tgaaaacggc aggagcatcc aatgaggcgg
      661 ttaaaaaggt gctgacaagc tttactggtg tgaagcaccg cctccaatat gtcacaactg
      721 tgaatggccg caaattttat aacgacagta aagcaacaaa cattctggcg acaagcaagc
      781 ggctttcagc ttttgataag ccggtcattc tgcttgcggg cggactggat cgcggaaatg
      841 gttttgatga cttgaaacct tatatgaagc atgtcaaagc agtccttaca tttggccaga
      901 cggcgccaaa actcgagaaa ctcggcaatg aactgggaat acaacatgtc aaacgtgtcg
      961 ataatgttga acaagcagta tctgcggcgt ttgcgctctc aaatgaagga gatgtcatcc
     1021 tgctgtcacc cgcgtgtgcg agctgggatc aattcaaaac atttgaagaa cgcggtgaca
     1081 tgtttataga tgccgtgcat atgcttaagt aagggcttgt cttgaagtaa atcggcagcc
     1141 ctaatgactt taagcttggg gtgtcgattg ctttgactac taaaaaaaca tcgcctgatt
//