From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human rearranged immunoglobulin lambda light chain mRNA
Date: 15 Aug 92 10:02:13 GMT
Distribution: bionet
Checksum: 27961     4

LOCUS       HUMIGVL029    819 bp ss-RNA             PRI       15-AUG-1992
DEFINITION  Human rearranged immunoglobulin lambda light chain mRNA
ACCESSION   X57819
KEYWORDS    Ig lambda light chain; immunoglobulin.
SOURCE      Homo sapiens RNA.
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Klobeck,H.G.
  JOURNAL   Unpublished (1991)
  STANDARD  full automatic
REFERENCE   2  (bases 345 to 391)
  AUTHORS   Combriato,G. and Klobeck,H.G.
  TITLE     V(lambda) and J(lambda)-C(lambda) gene segments of the human
            immunoglobulin lambda light chain locus are separated by 14kb and
            rearrange by a deletion mechanism
  JOURNAL   Eur. J. Immunol. 21, 1513-1522 (1991)
  STANDARD  full automatic
COMMENT     *source: tissue=spleen; *source: cell_type=B-cell; *source:
            clone_library=phage library cML; *source: clone=cML58; *source:
            isolate=individual ML; *source: chromosome=22; *source:
            is_germline=N;
            for overlapping sequences see: X51754-55; J00252-54; M15641-42
            From EMBL    entry HSIGVL029;  dated 15-JUL-1991.
FEATURES             Location/Qualifiers
     CDS             <1..709
                     /gene="immunoglobulin lambda light chain"
                     /codon_start=2
                     /translation="ASFPLLLTLLTHCAGSWAQSVLTQPPSASGTPGQRVIISCSGSS
                     SNIGGNTVNWYQQLPGRAPKLLIHSNNQRPSGVPDRFSGSKSGTSASLAISGLQSEDE
                     ADYYCAAWDDSLNGRYVFGTGTKVTVLGQPKANPTVTLFPPSSEELQANKATLVCLIS
                     DFYPGAVTVAWKADGSPVKAGVETTKPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQV
                     THEGSTVEKTVAPTECS"
     sig_peptide     <1..55
                     /gene="immunoglobulin lambda light chain"
                     /codon_start=2
     mRNA            1..819
                     /note="cDNA"
                     /gene="immunoglobulin lambda light chain"
                     /evidence=EXPERIMENTAL
     misc_feature    56..389
                     /note="V(lambda)I"
                     /gene="immunoglobulin lambda light chain"
     misc_feature    353..389
                     /note="J-segment"
                     /gene="immunoglobulin lambda light chain"
     misc_feature    390..709
                     /note="constant region"
                     /gene="immunoglobulin lambda light chain"
BASE COUNT      182 a    274 c    211 g    152 t
ORIGIN
        1 ggccagcttc cctctcctcc tcaccctcct cactcactgt gcagggtcct gggcccagtc
       61 tgtgctgact cagccaccct cagcgtctgg gacccccggg cagagggtca tcatctcttg
      121 ttctggaagc agctccaaca tcggaggtaa tactgtaaac tggtaccagc agctcccagg
      181 aagggccccc aaactcctca tccatagtaa taatcagcgg ccctcagggg tccctgaccg
      241 attctctggc tccaagtctg gcacctcagc ctccctggcc atcagtgggc tccagtctga
      301 ggatgaggct gattattact gtgcagcatg ggatgacagc ctgaatggtc ggtatgtctt
      361 cggaactggg accaaggtca ccgtcctagg tcagcccaag gccaacccca ctgtcactct
      421 gttcccgccc tcctctgagg agctccaagc caacaaggcc acactagtgt gtctgatcag
      481 tgacttctac ccgggagctg tgacagtggc ctggaaggca gatggcagcc ccgtcaaggc
      541 gggagtggag accaccaaac cctccaaaca gagcaacaac aagtacgcgg ccagcagcta
      601 cctgagcctg acgcccgagc agtggaagtc ccacagaagc tacagctgcc aggtcacgca
      661 tgaagggagc accgtggaga agacagtggc ccctacagaa tgttcatagg ttcccaactc
      721 taaccccacc cacgggagcc tggagctgca ggatcccagg ggaggggtct ctctccccat
      781 cccaagtcat ccagcccttc tccctgcact catgaaacc
//