From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Streptococcus agalactiae galactosyl transferase (cpsD) gene, 5'
Date: 26 Jan 93 05:53:26 GMT
Distribution: bionet
Checksum: 24245 8

LOCUS       STRCPSABD    3709 bp ds-DNA             BCT       25-JAN-1993
DEFINITION  Streptococcus agalactiae galactosyl transferase (cpsD) gene, 5'
            end, and cpsA and cpsB genes, complete cds.
ACCESSION   L09116
KEYWORDS    cpsA gene; cpsB gene; cspD gene; galactosyl transferase.
SOURCE      Streptococcus agalactiae (strain COH1) DNA.
  ORGANISM  Streptococcus agalactiae
            Prokaryotae; Firmicutes; Firmibacteria; Gram-positive cocci;
            Streptococcaceae.
REFERENCE   1  (bases 1 to 3709)
  AUTHORS   Rubens,C.E., Heggen,L.M., Haft,R.F. and Wessels,M.R.
  TITLE     Identification of cpsD, a gene essential for type III capsule
            expression in Group B Streptococci
  JOURNAL   Unpublished (1993)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     CDS             391..1122
                     /function="Unknown"
                     /gene="cpsA"
                     /citation=[1]
                     /note="putative"
                     /codon_start=1
                     /translation="MIDIHSHIVFDVDDGPKTLEESLSLIEESYRQGVRIIVSTSHRR
                     KGMFETPEDIIFKNFSIVKHETEKRFEHLQILYGGELYYTSDMLEKLKLKQIPTLNNT
                     KFALIEFSMQTSWKDIHTALSNVLMLGITPVVAHIERYNALENQKERVKEIINMGCYT
                     QINSFHILKQKLFNDKHKRFKKRARYFLEENLVHFVASDMHNLDVRPPFLAEAYKIIC
                     RDFGKERANQLFIENAQSILKNHYI"
     CDS             1131..1823
                     /function="Unknown"
                     /gene="cpsB"
                     /citation=[1]
                     /note="putative"
                     /codon_start=1
                     /translation="MNKIANTEVEINIFNLLKKLWKKKFLITFVAIAFATAGLFYSLF
                     IVTPQYTSSTRIYVINPNTPNNSITAQDLQAGSFLANDYKEIITSTDVLEKVISSEKL
                     NYPSSQLLQKITVSILKDTRVISISVEDANPKMSQKLANSVREAAVSKIKAVTQVEDI
                     TTLEKGNLPKAPSSPNIKKNVLIGFIVGAGLSTIVLVIMGILDDRVNTEEDIEKVLGL
                     TSLGIVPDLNKL"
     CDS             1834..2385
                     /function="Unknown"
                     /gene="cpsC"
                     /citation=[1]
                     /note="putative"
                     /codon_start=1
                     /translation="MTRLEIVDSKLRQAKKTEEYFNAIRTNIQFSGKENKILAIASVR
                     EGEGKSTTSTSLALSLAQAGFKTLLIDADTRNSVMSGTFKATGTIKGLTNYLSGNADL
                     GDIICETNVPRLMVVPSGKVPPNPTALLQNAYFNKMIEAIKNIFDYIIIDTPPIGLVV
                     DAAIIANACRWFYFSNPSRQNKT"
     CDS             2857..3679
                     /gene="cpsD"
                     /evidence="EXPERIMENTAL"
                     /citation=[1]
                     /partial
                     /product="galactosyl transferase"
                     /codon_start=1
                     /translation="MNSFLKYYRKYSYAKFSRDTKVVLITNKDSLSKMTFRNKYDHNY
                     IAVCILDSSEKDCYDLKHNSLRIINKDALTSELTCLTVDQAFINIPIELFGKYQIQDI
                     INDIEAMGVIVNVNVEALSFDNIGEKRIQTFEGYSVITYSMKFYKYSHLIAKRFLDIT
                     GAIIGLLICGIVAIFLVPQIRKDGGPAIFSQNRVGRNGRIFRFYKFRSMRVDAEQIKK
                     DLLVHNQMTGLMFKLEDDPRITKIGKFIRKTSIDELPQFYNVLKGDMSLAGTASHS"
BASE COUNT     1331 a    528 c    643 g   1207 t
ORIGIN      
        1 tgagcaggct ttgggctttg ttagagaacg ttattctcta caaggaggcg ataacgatag
       61 aggtagaaat caagaaaaag tgattgcagc tattataaat aagttagctt ctagtcagtc
      121 agtaacaaaa ttaaatagca ttacctcaca gctccaaacg tccgttcaaa ctaatatgac
      181 tattgataat attaatgatt tgattaacaa tcaattgtca actggacaac gcttcactgt
      241 cgagtcacaa gcattaactg gtcatggttc aacgggtgaa ctcccttcat atgcaatgcc
      301 aggagctcaa ctttatatga tgtcaattga tcaatctagc ttatctaatg caaaatcaaa
      361 aattaagaac acaatggagg aataacaaga atgattgata ttcattctca tatcgttttt
      421 gatgtagatg atgggcccaa aacgcttgaa gagagtttat ctttaataga agaaagttat
      481 cgacaaggtg ttagaataat tgtttcgaca tcacatagaa gaaaaggtat gtttgaaaca
      541 cctgaagata ttatcttcaa aaacttttct atagttaaac acgaaacgga aaaaagattt
      601 gaacatcttc aaatcttata tggtggagaa ttatactata caagtgatat gttagaaaaa
      661 ctgaagttaa agcaaattcc aactttaaac aatacgaaat ttgctttaat tgaattttct
      721 atgcaaactt cttggaaaga tattcataca gctttgtcaa atgttttaat gcttggtatt
      781 acaccagtcg ttgcgcatat agagaggtat aacgctttag agaatcaaaa agaacgggtg
      841 aaggaaatta ttaatatggg gtgttacaca caaataaata gtttccatat tttgaaacaa
      901 aaacttttta atgataagca taaacgcttt aagaaaagag cccgttattt tttagaggaa
      961 aatttagtgc attttgtagc gagtgatatg cataaccttg atgttagacc gccattttta
     1021 gcagaagctt ataagattat ctgtagagat ttcggtaaag aacgtgctaa ccaacttttt
     1081 attgaaaatg ctcaatctat attaaaaaac cattacattt aggagatttc atgaataaaa
     1141 tagctaatac agaagttgaa ataaatatat tcaacctttt aaaaaaattg tggaaaaaga
     1201 aatttttaat cacatttgtt gccattgcgt ttgcaacagc tggacttttc tatagtcttt
     1261 ttattgttac gccacaatat acttcttcaa caaggatata tgtcattaac cctaataccc
     1321 ctaataatag tattactgca caagatctac aagcggggag ttttcttgca aatgactata
     1381 aggagattat tacgtctact gacgttctag aaaaagttat ttcttctgaa aaattgaatt
     1441 atccttcgtc tcagttgcta caaaaaataa cagtttctat tttaaaagat acacgtgtta
     1501 tttcaatatc ggtcgaagat gctaatccaa aaatgtctca aaaattagca aattcagtta
     1561 gagaagcagc agtttcaaaa atcaaggcag ttactcaagt agaggatatc actactcttg
     1621 agaagggaaa tttacctaaa gcaccatctt ctcctaatat taaaaagaat gtactaatcg
     1681 ggtttattgt tggtgcagga ttatcaacta ttgttttagt tattatgggg attttggatg
     1741 atcgtgttaa tactgaagaa gatattgaaa aggtcttagg acttacttct ttaggtatag
     1801 taccagattt gaataaactt taaggagaat ataatgactc gtttagaaat agttgatagc
     1861 aagttaagac aagcaaaaaa aacagaagaa tacttcaatg cgatccgtac aaacatacag
     1921 tttagtggaa aggaaaataa aattcttgca attgcctctg ttagggaagg ggaaggaaaa
     1981 tccactactt caacaagttt agctttatct ttagctcaag caggatttaa aacattatta
     2041 attgatgcgg atactaggaa ctctgttatg tctggaacct ttaaagcaac tggaactatt
     2101 aaaggcttga cgaattattt atcaggtaat gcagatcttg gagatattat ctgtgaaacc
     2161 aatgttccta gactgatggt cgttccttca ggaaaagtac caccaaatcc aacagcatta
     2221 cttcagaacg cttattttaa taagatgatt gaagctatta aaaatatatt tgattatatt
     2281 atcatcgata ctccacctat tggtttagtt gttgatgccg caataatcgc taatgcttgt
     2341 cgatggtttt attttagtaa cccaagcagg cagaataaaa cgtaattatg ttgaaaaagc
     2401 aaaagaacag atggaacaaa gtggttcaaa ttcttaggta ttattcttaa taaagttaat
     2461 gaatctgttg ctacttacgg cgattatgga aattacggaa aaagggatag aaaaaggaag
     2521 taagggactc tggtattgaa agaaaaagaa aatatacaaa agattattat agcgatgatt
     2581 caaacagttg tagtttattt ttctgcaagt ttgacattaa cattaattac tcccaatttt
     2641 aaaagcaata aagatttatt gtttgttcta attgatacat tatattgttt tttatctttc
     2701 tgatttttac agagactttt ggagtcgtgg ctatcttgaa gagtttaaaa tggtattgaa
     2761 atacagcttt tactatattt tcatatcaag tcattatttt ttatttttaa aaactctttt
     2821 acaacgacac gactttcctt ttttactttt attgctatga attcattttt aaaatattat
     2881 cgaaaatatt cttacgctaa gttttcacga gataccaaag ttgttttgat aacgaataag
     2941 gattctttat caaaaatgac ctttaggaat aaatacgacc ataattatat cgctgtctgt
     3001 atcttggact cctctgaaaa ggattgttat gatttgaaac ataactcgtt aaggataata
     3061 aacaaagatg ctcttacttc agagttaacc tgcttaactg ttgatcaagc ttttattaac
     3121 atacccattg aattatttgg taaataccaa atacaagata ttattaatga cattgaagca
     3181 atgggagtga ttgtcaatgt taatgtagag gcacttagct ttgataatat aggagaaaag
     3241 cgaatccaaa cttttgaagg atatagtgtt attacatatt ctatgaaatt ctataaatat
     3301 agtcacctta tagcaaaacg atttttggat atcacgggtg ctattatagg tttgctcata
     3361 tgtggcattg tggcaatttt tctagttccg caaatcagaa aagatggtgg accggctatc
     3421 ttttctcaaa atagagtagg tcgtaatggt aggattttta gattctataa attcagatca
     3481 atgcgagtag atgcagaaca aattaagaaa gatttattag ttcacaatca aatgacaggg
     3541 ctaatgttta agttagaaga tgatcctaga attactaaaa taggaaaatt tattcgaaaa
     3601 acaagcatag atgagttgcc tcaattctat aatgttttaa aaggcgatat gagtttagca
     3661 ggaaccgcct cccacagttg atgaatatga aagtataatt cacgcgagg
//