From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Trypanosoma brucei (clone WaTat1.1, g2.50kb DraI fragment) surface
Date: 24 Jul 92 10:10:37 GMT
Distribution: bionet
Checksum: 48728     4

LOCUS       TRBSURGLYH   1239 bp ds-DNA             INV       24-JUL-1992
DEFINITION  Trypanosoma brucei (clone WaTat1.1, g2.50kb DraI fragment) surface
            glycoprotein (VSG) gene, 5'end.
ACCESSION   M83701
KEYWORDS    surface glycoprotein.
SOURCE      Trypanosoma brucei DNA.
  ORGANISM  Trypanosoma brucei
            Eukaryota; Animalia; Protozoa; Sarcomastigophora; Mastigophora;
            Kinetoplastida; Trypanosomatina; Trypanosomatidae.
REFERENCE   1  (bases 1 to 1239)
  AUTHORS   Barbet,A.F., Davis,W.C. and McGuire,T.C.
  TITLE     Cross-neutralization of two different trypanosome populations
            derived from a single organism
  JOURNAL   Nature 300, 453-456 (1982)
  STANDARD  full staff_review
REFERENCE   2  (bases 1 to 1239)
  AUTHORS   Barbet,A.F., Myler,P.J., Williams,R.O. and McGuire,T.C.
  TITLE     Shared surface epitopes among trypanosomes of the same serodeme
            expressing different variable surface glycoprotein genes
  JOURNAL   Mol. Biochem. Parasitol. 32, 191-200 (1989)
  STANDARD  full staff_review
REFERENCE   3  (bases 1 to 1239)
  AUTHORS   Kamper,S.M. and Barbet,A.F.
  TITLE     Surface epitope variation via mosaic gene formation is potential
            key to long-term survival of Trypanosoma brucei.
  JOURNAL   Mol. Biochem. Parasitol. 53, 33-44 (1992)
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     CDS             1..1239
                     /standard_name="VSG"
                     /product="surface glycoprotein"
                     /gene="VSG"
                     /evidence=EXPERIMENTAL
                     /codon_start=1
                     /translation="MTKSPTKLSQVALSYLLLIAAAPSHVQPASQSALDKGLWEGICK
                     ATAELNKVPGTMTHELTQILQKAKTMRRGELKAQIFGLRHAGTELAKRAAVMAAYFGR
                     RHNQMLATLETKDTEKATDAAAKAAYLQGRIDDPLHLLAAVDNTNNLFLAVTNAAGSK
                     PVKTEGKLGDFDCPLSAPSAKNKAAELSGITEAGFTMLKTDVTSGNAKQVASGAKKCN
                     LLSSVNTQGFGETQAAISTDHEILGGYITIKNTNTEIDVSGHDKTHTEERDKHQSWTV
                     AHAAIMVSPKHTTSPYANETGEIHSRPDMTKVLQAVYSAXXPISGTDLENKVIEIFQG
                     KEEKKLQEYEDAVNQVTIPAGVAGLETDQTLAAVNDEDKLIAIRAFYEQQVSAXYGKM
                     VATIAEASNKQTATAPADCGK"
                     /citation=[2]
                     /citation=[1]
                     /citation=[3]
                     /partial
BASE COUNT      443 a    315 c    303 g    174 t      4 others
ORIGIN
        1 atgacaaaaa gtccaacgaa actgagccaa gtagcgcttt catacttgtt actaatcgcc
       61 gcagcaccga gtcatgtaca acccgctagt caatcagcct tggacaaagg gctctgggag
      121 ggcatatgca aagcgacggc agagctgaac aaagttcccg ggacgatgac gcacgaattg
      181 acgcagatcc tccaaaaagc gaagacgatg cggcgtggag aactgaaagc acaaatattc
      241 gggctaaggc atgcaggaac agaattggcc aagagagcag cggtaatggc ggcatatttc
      301 ggccgtaggc acaatcagat gcttgcaacg cttgaaacga aagacaccga aaaggcaacc
      361 gacgcggcag ccaaagcagc gtaccttcaa ggcagaatag acgacccact tcatttactg
      421 gcagctgtgg ataacacaaa caaccttttt ctagctgtaa ccaacgcagc cggcagcaaa
      481 ccagtcaaaa cagagggtaa actcggcgac ttcgactgcc cactgtcggc gccgtcagct
      541 aaaaacaagg cagccgaatt aagcggtata acagaggccg gctttaccat gctaaaaacg
      601 gatgtgacca gcggcaacgc aaaacaggtc gcgagcggcg caaagaaatg caacctacta
      661 agttcagtaa acacccaagg cttcggagag acacaggcag cgatcagcac cgaccacgaa
      721 attctcggag gatacataac aataaaaaac acaaacactg aaatagacgt aagcggacac
      781 gataagacac acacagaaga acgcgacaaa caccaaagct ggacggtggc acatgcagcc
      841 attatggtga gcccaaaaca cacaacgagc ccctatgcca acgaaactgg agagattcac
      901 agcaggccgg acatgacaaa agtgttgcaa gccgtttaca gcgcaaassc gccgataagc
      961 ggcaccgacc tagagaacaa ggtcatagaa atatttcaag gaaaagaaga aaagaaacta
     1021 caagaatacg aagacgctgt aaaccaagta acgataccag ccggcgtagc aggactggaa
     1081 actgaccaga cgctagcagc agtaaacgac gaagacaaac taatcgcaat cagagccttt
     1141 tatgaacagc aggtcagcgc assctatggc aaaatggtag cgacgatagc cgaagcaagc
     1201 aataaacaaa cagccaccgc cccagcagac tgcggtaaa
//