HDTenEightyP/NetNews huggingface dataset:
From: ALSOBROOK@biomed.med.yale.edu (John Alsobrook)
Subject: Thesit is Not Magic
Organization: Yale University
Date: Thu, 23 Jul 1992 14:11:55 GMT
X-News-Reader: VMS NEWS 1.20

Greetings.  After my recent flame on mis-interpretation of a PCR methods paper
in NAR ( Ponce and Micol, NAR 20(3):623, 1992 ), I received a few requests to
post general info on the "mistakenly magical" ingredient Thesit.

Thesit is a trade name for Polyoxyethylene(9)-lauryl-ether (available
from Sigma, and probably other places as well). The structural formula
(based on similar compounds in the Aldrich catalog) seems to be:

		C  H  (OCH CH ) OH
		 12 25    2  2 9    , 

but it's been awhile since my IUPAC nomenclature was up to snuff, so I hope the
*REAL* chemists give me a break here. Thesit is a waxy solid at room temp, and 
is listed as a skin and respiratory irritant. The Aldrich chemical catalog
lists several other similar compounds (varying numbers of oxyethylene units in
the chain) as Brij 30, Brij 35, etc., which are relatives of the Tween family. 

So the question now is why did these investigators decide to include
Thesit in *ALL* of their PCR reactions along with the more standard reaction
components?  Is this just SOP in Eric Davidson's lab?  Does anyone know of
other PCR papers that mention Thesit?

****************************************************************************
*                                       | "Giving money and power to the   *
* John Alsobrook                        |    government is like giving     *
* Child Study Center                    |     whiskey and car keys to      *
* Yale University School of Medicine    |  teenage boys." - P.J. O'Rourke  *
*--------------------------------------------------------------------------*
*                       blame me, not my parents!                          * 
*               internet:alsobrook@biomed.med.yale.edu                     *
*--------------------------------------------------------------------------*
...and, of course, nothing I ever say is officially sanctioned by Yale...
From: masuda@fcs280b.ncifcrf.gov (Michiaki Masuda)
Newsgroups: bionet.molbio.methds-reagnts
Subject: Unclonable sequences
Date: 23 Jul 92 02:58:23 GMT
Organization: Frederick Cancer Research and Development Center


  There seem to be some retroviral sequences that cannot be cloned in a phage
or a plasmid vector successfully. An infectious molecular clone of HTLV and
some strains of MMTV have not yet been isolated. 
  
  I think I heard about other examples of unclonable sequences such as some 
inverted repeat sequences.
  
  I wonder if there's a review article or some compiled information regarding
unclonable seqeunces. I'd appreciate it if someone could enlighten me in this
regard.

Michiaki Masuda
Laboratory of Molecular Oncology
National Cancer Institute
Frederick, MD 21702
From: pnh@fcsparc6.ncifcrf.gov (Paul N Hengen)
Newsgroups: bionet.molbio.methds-reagnts
Subject: Tracer Dyes in Agarose
Summary: What are these?
Keywords: Tracer Dyes
Date: 27 Jul 92 18:04:36 GMT
Distribution: bionet
Organization: Frederick Cancer Research and Development Center

Hello. For some time I've been using a ficoll based loading
solution for my agarose gels with both xylene cyanol and
bromphenol blue added as tracer dyes. Recently, I saw an
advertisement for a loading dye mix of three dyes used.
When I called the company which sells these, they would not
tell me what the dyes are. BUT, they told me that they are
NOT the two mentioned above...So, I ask if anyone knows of
any other dyes that can be used for this purpose? In particular
I'm wondering if anyone uses a dye that migrates faster than the
bromphenol blue front?


Paul N. Hengen
National Cancer Institute
Frederick Cancer Research and Development Center
Laboratory of Mathematical Biology
P.O. Box B
Frederick, Maryland 21702-1201 USA
voice: (301) 846-5581
FAX: (301) 846-5598
e-mail: pnh@ncifcrf.gov
Newsgroups: bionet.molbio.methds-reagnts
From: djt2@po.CWRU.Edu (Dennis J. Templeton)
Subject: Re: Separation of DNA fragments.
Reply-To: djt2@po.CWRU.Edu (Dennis J. Templeton)
Organization: Case Western Reserve University, Cleveland, OH (USA)
Date: Fri, 31 Jul 92 15:43:43 GMT


In a previous article, jaf@bio.aau.dk () says:

>How can two DNA fragments  of MW 3300 kb and 3400 kb be separated on an agarose
>gel?? They have to be separated so much that one can isolate them without
>contamination. 
>

jaf-  I have to disagree with a posted followup that says to use a 4% 
gel; this would work with a smaller fragment but not with 3.4k.

We have had good luck by digesting the set with an enzyme that cuts in the
middle of the piece you *don't* want, effectively removing it from the
piece you do.  It's surprising how often a good enyme for this can be
found.

Often too, judicious use of CIP can remove the effects of a small level of
contamination... for example, are you preparing a vector for cloning?  then
CIP the mix and any contaminating fragment should be unligatable.

My rule of thumb is that it is difficult to resolve pieces that differ by
less than 10%.  Also, contamination seems to smear *up* the gel.  If you
are separating pieces of 4kb and 3 kb, the 4 kb will be contaminated with
some of the 3kb, but the 3 kb will be much purer; the front running band is
cleaner (in my experience)

good luck,

dennis
From: skspoidn@uk.ac.rdg.susssys1 ("Mike Poidinger")
Newsgroups: bionet.molbio.methds-reagnts
Subject: TBE and other basics
Date: 23 Jul 92 17:49:14 GMT
Distribution: bionet
Original-To: methods@uk.ac.daresbury

I'll go direct (certainly not worth posting here :-)

Mike Poidinger
Dept of Microbiology 
University of Reading
From: muriana@aclcb.purdue.edu (Peter M. Muriana)
Newsgroups: bionet.molbio.methds-reagnts
Subject: Re: Ecoli strains
Date: 23 Jul 92 13:44:34 GMT
Reply-To: muriana@aclcb.purdue.edu
Distribution: bionet
Organization: Purdue University AIDS Center

In article <23013.9207231111@crc.ac.uk>, sbhattac@mrc-crc.ac.uk (Dr. Shoumo Bhattacharya B1 061) writes:
>Can anyone tell me of a source where I can find E.coli strains and 
>their genotypes?  Also are E. coli GM48 methylation defective (dam/dcm)? Many thanks  
>Shoumo Bhattacharya

You are likely to find such a list in the Maniatis 3-volume set or 
in the "Red Book (Protocols in Mol. Biol)"

Also, usually in the back of the catalogues of the Molecular Biology suppliers 
such as:
			BRL, p. 718
			Stratagene, p.237 = E.coli GM48
			New Eng. Biolabs, p.202, and so forth

	E. coli GM48 = thr, leu, thi-1, lacY, galK, galT, ara, tonA, tsx,
			**dam**, **dcm**, supE44.

hope this helps.
*********************************************************************
*  Peter M. Muriana, Ph.D.		Phone	= (317)-494-8284    *
*  Dept. of Food Science		FAX	= (317)-494-7953    *
*  Purdue University		E-mail	= muriana@aclcb.purdue.edu  *
*  W. Lafayette, IN  47907                                          *
*********************************************************************
From: naffara@crc.ac.uk (Dr. N.A. Affara)
Newsgroups: bionet.molbio.methds-reagnts
Subject: Vector sequence wanted!!!
Date: 21 Jul 92 11:58:30 GMT
Organization: MRC Human Genome Resource Centre

Come on!
Somebody out there must have the sequence for these vectors!
Just to remind you they are pJLA503, pJLF201.

I need these as soon as possible so please don't just ignore this posting!

If you haven't got the actual sequence, can you tell me where I might find
it myself.

PLEASE HELP

Thanks,
Phil
(E-mail naffara@uk.ac.crc)
Newsgroups: bionet.molbio.methds-reagnts
From: broe@aardvark.ucs.uoknor.edu (Bruce Roe)
Subject: Re: SEQUECING PCR PRODUCT
News-Software: VAX/VMS VNEWS 1.41    
Organization: University of Oklahoma - University Computing Services
Date: Fri, 31 Jul 1992 12:49:00 GMT

In article <1992Jul31.103133.1@lure.latrobe.edu.au>, bioysl@lure.latrobe.edu.au writes...
>HI! FRIENDS!
> 
>   COULD ANYONE TELL ME ANY METHODS WHICH I CAN DIRECTLY SEQUECE MY PCR PRODUCT
>WITHOUT CLONING IT FIRST?
> 
>   THANKS IN ADVANCE.
> 
>YONHSHU LIU
>BIOCHEM. DEPT
>LA TROBE UNI. 
>AUSTRALIA

	Several methods, radioactive and fluorescent, using every Polymerase
know have been used to directly sequence PCR products.  Think of the PCR
product as being double stranded DNA, go to the library, and READ the
literature.
	Use double stranded DNA as a positive control for your experiments
because sequencing off PCR products is more of an art than a science, and
keep in mind that the purity of the template is the key to good sequence
data.  To paraphrase an old statement:

	 "Don't waste good thoughts on dirty DNA"

Cheers and good luck...........bruce
  - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
 \  Bruce A. Roe               Professor of Chemistry and Biochemistry /
 /  University of Oklahoma     INTERNET: BROE@aardvark.ucs.uoknor.edu  \
  - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  - - -
Newsgroups: bionet.molbio.methds-reagnts
From: broe@aardvark.ucs.uoknor.edu (Bruce Roe)
Subject: Re: What happens to 32P-labelled compounds?
Followup-To: bionet.molbio.methds-reagnts
News-Software: VAX/VMS VNEWS 1.41    
Organization: University of Oklahoma - University Computing Services
Date: Fri, 31 Jul 1992 11:46:00 GMT

In article <3660@fcs280s.ncifcrf.gov>, toms@fcs260c2.ncifcrf.gov (Tom Schneider) writes...
>In article <1992Jul30.085812.18345@polaris.utu.fi>
>eepee@polaris.utu.fi (Esa-Pekka P{lvim{ki) writes:

	<stuff deleted>

>An interesting question on these lines is:
> 
>Suppose one labeled some DNA with 32P.  Then look under an STM.  Watch again.
>Watch again.   POOF!  Look again and there is a little crater there or what?
>This experiment is easy to do and one would be (I think) the first person
>to observe the radioactive decay of a single molecule.  If someone does
>the experiment I'd like to know the result.  (I ran some calculations on this
>a while back, and it seems like one would not need to wait very long to see
>some molecules decay if one watched a large enough set of them.  Half life
>of 32P is only 2 weeks.)

IMHO you would see a 32S where there once was a 32P but *no crater*.
Lest we forget, the radiolabeled molecules we use as "tracers" usually
contain a very large excess of non-radiolabeled carrier and the actual
number of molecules which decay in the population is quite small compared
to those that do.  BTW, 32S is a stable isotope of sulfur so there would
be no further decay of those atoms.

A simplier experiment than Toms, would be to lable a batch of DNA with
alpha-32P-dATP and then run a series of gels (say 1 every other day)
and look to see if the DNA falls appart in time as the 32P goes to 32S.
My guess is if the tube were not contaminated with nucleases you'd not
see any degredation.

;-)

Cheers -- bruce
  - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
 \  Bruce A. Roe                     Dept. Chemistry and Biochemistry /
 /  BROE@aardvark.ucs.uoknor.edu     University of Oklahoma           \
  - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
Newsgroups: bionet.molbio.methds-reagnts
From: gat44@iastate.edu (Gabriel Trueba)
Subject: Re: ethanol precipitation
Organization: Iowa State University, Ames, IA
Distribution: bionet
Date: Thu, 23 Jul 1992 02:10:43 GMT

 We are having some problems using some pcr products as
probes for Southern blot analysis. I wonder whether somebody
in the net has experimented lack of hybidization of PCR products
We would appreciate any suggestion.

Thank you
From: WMELCHIOR@NTET.NCTR.FDA.GOV (Bill Melchior, NCTR/FDA)
Newsgroups: bionet.molbio.methds-reagnts
Subject: need pMB9 sequence
Date: 21 Jul 92 20:22:33 GMT
Distribution: bionet

I have the feeling that it should be obvious, but I can't find a sequence
for pMB9 in any of my catalogs or at Genbank.  Does anyone know where I 
can find it?

Thank you.
________________________________________________________________________________
The opinions stated are mine, not those of NCTR or its sponsoring organizations.

Bill Melchior                                ||   "You have lawyers the way
National Center for Toxicological Research   ||    other people have mice."
Jefferson, AR  72079                         ||
(501) 543-7206                               ||   -Kenneth Duncan, English
                                             ||    Health & Safety Executive,
WMELCHIOR@NTDOC.NCTR.FDA.GOV                 ||    to US regulators
Newsgroups: bionet.molbio.methds-reagnts
From: kim@m44.unm.edu
Subject: Re: Charms (Was: Tracer dyes in agarose gels)
Date: Fri, 31 Jul 92 18:01:59 GMT
Organization: UNM Cancer Center, Albuquerque, NM
Reply-To: kim@m44.unm.edu

In article <1992Jul30.155336.29815@usenet.ins.cwru.edu>, djt2@po.CWRU.Edu (Dennis J. Templeton) writes:
>
>In a previous article, themmen@nl.eur.fgg.endov ("Axel P.N. Themmen") says:
>>
>> Yes There is such a dye. It is Orange G (Sigma O 1625). We use it instead
>> of the bromophenolblue and xylene cyanol in Ficoll based loading dye.
>> Works as a charm.
>        ^^
>
>Axel probably doesn't have the (?dis)advantage of hearing people say often:
>"works *like* a charm", but his version is refreshing and maybe more accurate.
>
Or, for those not too young, how many remember the jingle: "Winston tastes good
like a cigarette should"?  and the advertising campaign in which Winston
smokers debated AS vs LIKE?

Daniel Kim
KIM@FLOVAX.LANL.GOV

Newsgroups: bionet.molbio.methds-reagnts,bionet.general
From: gchacko@magnus.acs.ohio-state.edu (George W Chacko)
Subject: Free Tritiated Water!!!
Organization: The Ohio State University
Date: Thu, 23 Jul 1992 18:12:53 GMT


I;m posting this for SOMEONE ELSE so please address followups to
KAMAYNE@VASSAR.EDU.

---------------------------------------------------------------------------
Subject: Free Tritiated Water


Please forward this note to any colleague at ANY school or other
institution.  Even if your contact at that school does not use tritiated
water, they may know someone who does.

I have a large amount (1.5 curies) of tritiated (radioactive) water that I
would like to GIVE to some lab that can use it.  It is worth over $500 if
bought new, but will cost us about $5000 to discard as radioactive waste. 
We'd rather spend the money on shipping it to someone who will use it.  So,
could you ask around and ask your friends there to ask around for anyone
who uses the stuff?  Could you ask someone in your radioactive safety
office if there are big users at your institution that you may not know of?

The particulars on the tritiated water:
-Still in original sealed vials from NEN/Dupont
-Bought in 1979-80 as 2.9 Curies, so it is now about 1.5 curies.  The NEN
technical people say that the tritium decay should not affect the water
chemically.
Configuration: (before decay)
4 vials,	0.5 grams each (0.5 ml)	500 mCi  	2000 mCi total
9 vials,	1.0 grams each (1 ml)	100 mCi    	900 mCi total

Anyone interested should contact me by September 1st.

Thanks,
Kath Mayne
Vassar College Biology Department
Poughkeepsie, NY  12601
914-437-7434
KAMAYNE@VASSAR.EDU
KAMAYNE@VASSAR (BITNET)
From: pnh@fcs260c2.ncifcrf.gov (Paul N Hengen)
Newsgroups: bionet.molbio.methds-reagnts
Subject: Re: Smiles on Southerns
Keywords: Smiles
Date: 31 Jul 92 16:40:48 GMT
Distribution: bionet
Organization: Frederick Cancer Research and Development Center

In article <158hl8INN5dj@agate.berkeley.edu> scottk@nuclease.berkeley.edu (Number Ten Ox) writes:
>In article <3653@fcs280s.ncifcrf.gov>, pnh@fcs260c2.ncifcrf.gov 
>(Paul N Hengen) writes:
>
>|> -> Problems can occur when there is overloading with DNA, the
>|> concentration of agarose is too high, or you've pushed it so
>|> that the gel runs faster than normal. This causes a trailing
>|> effect, mostly due to shearing of the DNA. If you're using very
>                         ^^^^^^^^^^^^^^^^^^^
>
>Huh? Can you explain how shearing the DNA causes it to *trail*
>on a gel?
>

Oops. I realize that the original posting was for the electrophoresis
of restriction fragments of total genomic DNA, or was it?

Here, I was referring to a method of separating large plasmid DNA
(cccDNA) using a cell lysis technique within the well of an agarose
gel for use in Southern blots. Shearing and/or nicking of the cccDNA
in such a gel causes a trailing effect.

The method is described in the following book chapter:

L.D. Kuykendall and P.N. Hengen. 1988. Microbial Genetics
of Legume Root Nodulation and Nitrogen Fixation. In: Biological
Nitrogen Fixation: Recent Advances. N.S. Subba Rao (ed.). Oxford and
IBH Publishers, New Delhi.

Paul N. Hengen
National Cancer Institute
Frederick Cancer Research and Development Center
Laboratory of Mathematical Biology
P.O. Box B
Frederick, Maryland 21702-1201 USA
voice: (301) 846-5581
FAX: (301) 846-5598
e-mail: pnh@ncifcrf.gov
From: CZJ@CU.NIH.GOV
Newsgroups: bionet.molbio.methds-reagnts
Subject: Re:  Sodium phosphate buffer
Date: 22 Jul 92 12:19:05 GMT
Distribution: bionet

>
>        I usually assume that any dilution of a 0.2M solution will not change
> the pH provided that nanopure, or equivalent, water is used.
>        I hope this is of some help.
>
>                                                Alex Jeffries
>
>
> -------------------------------------------------------------------------------
>
>

The pH would actually change on dilution because the ionic strength
would change and the pKa of phosphate changes as a function of pH.

Jim Cassatt

Newsgroups: bionet.molbio.methds-reagnts
From: horton@molbio.cbs.umn.edu (Robert Horton)
Subject: Re: Home-made PCR gems
Organization: University of Minnesota
Date: Fri, 24 Jul 1992 18:41:37 GMT

I've also used ParaPlast tissue embedding medium(Oxford Labware, St.Louis)
which is "a compound of purified paraffin and plastic polymers of regulated
molecular weight". It seems to be a little less brittle at room temp than 
plain paraffin, and it doesn't seem to affect PCR.
-- 
Bob Horton            /\ "Crash programs fail because of the theory that
U. of Minnesota, CBS  || with nine women pregnant you get a baby a month" 
1479 Gortner Ave.    /||\   -Werner von Braun.  Disclaimer:"Bob who?"
St. Paul, MN 55108    ^^   horton@molbio.cbs.umn.edu/(612) 624-3790
Newsgroups: bionet.molbio.methds-reagnts
From: Ola Myklebost
Subject: Re: Tracer Dyes in Agarose
Organization: The Norwegian Radium Hospital ICR
Date: Fri, 31 Jul 1992 10:23:51 GMT

In article <3643@fcs280s.ncifcrf.gov> pnh@fcsparc6.ncifcrf.gov (Paul N Hengen)
writes:
.
.
>I'm wondering if anyone uses a dye that migrates faster than the
>bromphenol blue front?

We use Xylene Xyanole FF (Sigma X-0377)


Newsgroups: bionet.molbio.methds-reagnts
From: robert@arbo.microbiol.uwa.oz.au (Robert Coelen)
Subject: Re: Smiles on Southerns
Organization: University of Western Australia
Date: Wed, 29 Jul 1992 03:40:16 GMT

In article <d3mmcsa@lynx.unm.edu> kim@m44.unm.edu writes:
>In article <1992Jul28.110853.24275@gserv1.dl.ac.uk>, MORRIS@ch.unige.cmu ("Mike Morris, Geneva") writes:
>>Hi netters
>>
>>Why do bands smile (not across the gel, but within each band) on 
>>genomic Southerns?
>>A few years back, I switched from ficoll to sucrose loading dyes,
>>cos conventional wisdom said the problem would go away. CW was right,
>>but the problem has recently and suddenly returned.
>>The smiles are worst when there is a lot of DNA (or perhaps salt in
>>the DNA sample?) ina a lane. 
>>Explanations and tips appreciated!
>
>I don't know but I've been told . . .

I suspect that the comment you made in brackets above '(or perhaps salt in...)'
is correct. Let's not forget that these molecules are moving in a force field
which is setup by the potential applied to the electrodes. Temperature also has
an effect on the speed with which DNA runs through the gel. If you want to see
a really hot gel, just wait until one of your graduate students runs a gel with
10x (instead of 1x) buffer. All the effort that we used to go through to maintain
an even temperature across the sequencing gel was to avoid the dreaded smile.
In the centre of the well the concentration of salts that came with your DNA would
be higher thereby locally raising the temperature. So why not wash your DNA pellet
with 70% ethanol in plain old water after you've pelleted it. I know its a pain, but
it does assist in lowering the salt concentration of the sample and may help
prevent the intraband smile. A great way to reduce your salt is to glass milk clean
your sample and redissolve your DNA in TE......


Robert Coelen
Department of Microbiology
The University of Western Australia
From: MMELAN@LUCY.WELLESLEY.EDU (Melissa Melan)
Newsgroups: bionet.molbio.methds-reagnts
Subject: Re: Separation of DNA Fragments
Date: 31 Jul 92 17:05:00 GMT
Distribution: bionet


>How can two DNA fragments  of MW 3300 kb and 3400 kb be separated on an agarose
>gel?? They have to be separated so much that one can isolate them without
>contamination.
>            

	If you want to separately subclone the fragments, I have found that it
is easier to cut out and gel purify the barely resolved bands, subclone then
select the clones by size afterwards.  It is usually easier to differentiate
the two bands if they are side by side rather than on top of each other.  You
should also adjust the agarose concentration to 0.7 or 0.5%. It should give
a better separation of the closely sized larger fragments.  Good Luck.
===============================================================================
Melissa Melan				Internet:(mmelan@lucy.wellesley.edu)
Department of Biological Sciences
Wellesley College
Wellesley, MA  02181
===============================================================================
From: herzer@urz.unibas.ch
Newsgroups: bionet.molbio.methds-reagnts
Subject: Mutation Selection
Date: 30 Jul 92 14:48:03 MET
Organization: University of Basel, Switzerland

I would like to look at the mutation rate of certain strains of E. coli
and am looking for genes within E. coli for which mutations could be
screened for efficiently.  For example, a gene which aquires a mutation
such that it does grow on a certain plate whereas the wild-type would
die; or color difference, etc.  Any help would be most appreciated.

                                  Sincerely,
                                             Peter
                                             "Herzer@urz.unibas.ch"
From: kim@m44.unm.edu
Newsgroups: bionet.molbio.methds-reagnts
Subject: Re: Smiles on Southerns
Date: 28 Jul 92 18:45:39 GMT
Reply-To: kim@m44.unm.edu
Distribution: bionet
Organization: UNM Cancer Center, Albuquerque, NM

In article <1992Jul28.110853.24275@gserv1.dl.ac.uk>, MORRIS@ch.unige.cmu ("Mike Morris, Geneva") writes:
>Hi netters
>
>Why do bands smile (not across the gel, but within each band) on 
>genomic Southerns?
>A few years back, I switched from ficoll to sucrose loading dyes,
>cos conventional wisdom said the problem would go away. CW was right,
>but the problem has recently and suddenly returned.
>The smiles are worst when there is a lot of DNA (or perhaps salt in
>the DNA sample?) ina a lane. 
>Explanations and tips appreciated!

I don't know but I've been told . . .

Stratagene makes a special comb for Agarose DNA gels in which the trailing
corners of the well are set in from the leading corners ( the back of the well
is narrower than the front  --------
                           /        \
                           ----------    Please forgive lousy ASCII drawing.

The idea is that DNA loaded into a well will diffuse slightly into the agarose
sides of the well.  When the current is applied, DNA that diffused into the
back of the well exits the agarose and speeds through the buffer in the well to
the leading edge.  DNA that is trapped in the sides has to actually migrate
through an extra millimeter of agarose than the stuff in the leading edge of
the well, leading to a considerable delay in the migration of that DNA, and
making it hang back from the rest of the band, hence the "smile".  This may
also account for "flaming" up the sides of the lane in an overloaded gel.

If you're in the mood to do a little gel apparatus surgery, you can whittle the
teeth of your gel combs to approximate the cross section of the special
Stratagene combs and see if this really is the case.

Daniel Kim
KIM@FLOVAX.LANL.GOV
Newsgroups: bionet.molbio.methds-reagnts,sci.chem,sci.misc