From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Pseudomonas putida aspartate carbamoyltransferase (pyrB) gene,
Date: 28 Aug 92 10:01:06 GMT
Distribution: bionet
Checksum: 31853     4

LOCUS       PSEPYRB      1403 bp ds-DNA             BCT       28-AUG-1992
DEFINITION  Pseudomonas putida aspartate carbamoyltransferase (pyrB) gene,
            complete cds.
ACCESSION   M97253
KEYWORDS    aspartate carbamoyltransferase; dihydro-orotase; pyrB gene;
            pyrC gene.
SOURCE      Pseudomonas putida (strain PpN-1) DNA.
  ORGANISM  Pseudomonas putida
            Prokaryotae; Gracilicutes; Scotobacteria; Pseudomonadaceae.
REFERENCE   1  (bases 1 to 1403)
  AUTHORS   Schurr,M.J., Vickrey,J.F., Benjamin,R.C., Shanley,M.S., Cunin,R.
            and O'Donovan,G.A.
  TITLE     Cloning and Nucleotide Sequence of pyrBC genes of P. putida
            encoding Aspartate transcarbamoylase and Dihydroorotase
  JOURNAL   Unpublished (1992)
  STANDARD  full staff_entry
FEATURES             Location/Qualifiers
     CDS             399..1403
                     /EC_number="2.1.3.2"
                     /product="aspartate carbamoyltransferase"
                     /gene="pyrB"
                     /codon_start=1
                     /translation="MTPIDAKRPLQLNDQGQLRHFLSLDGLPRELLTEILDTADSFLE
                     VGARAVKKVPLLRGKTVCNVFFENSTRTRTTFELAAQRLSADVISLNVSTSSTSKGET
                     LFDTLRNLEAMAADMFVVRHSDSGAAHFIAEHVCPDVAVINGGDGRHAHPTQGMLDML
                     TIRRHKGSFENLSVAIVGDILHSRVARSDMLALKALGCPDIRVIGPKTLIPIGIEQYG
                     VKVYTDLAEGLKDVDVVIMLRLQRERMAGGLLPSEGEFYRLFGLTTARLACAKPDAIV
                     MHPGPINRGVEIESAVADGKHSVILNQVTYGIAVRMAVLSMAMSGQNAQRQFDQENAQ
                     "
     misc_feature    1400..1403
                     /note="GTG is start of the pyrC of P. putida. The start of
                     pyrC overlaps the termination of pyrB by 4 bp"
                     /product="'Dihydro-Orotase'"
BASE COUNT      246 a    510 c    417 g    230 t
ORIGIN
        1 cctgcaggaa gccatgggcg acacgagccc catgggcacc ctggacgtct cgttctaccg
       61 cgacgacttc agccagaacg gcctgcaccc gcaagttcgc ccgtccgagc tgcctttcga
      121 ggtcgagggc agcacctggt gctggtggat gacgtactga tgggcggtcg caccatccgc
      181 ccggcgctca acgaactgtt cgattacgcc gcccggccag cgtcaccctg gtctgcctgc
      241 tggacctgga tgccggcgaa ttgccgatcc gtccgaatgt gctcggtgcc accctgtcgc
      301 tggccgccca tgaacgggta aaattgaccc ggacccgcac cgctcgccct cgagcgccag
      361 gacctgcctc gccttccgcc ctttaagagt cccccgcgat gacgccaatc gacgccaagc
      421 gcccgctgca gctcaatgat cagggccagc tgcgccactt cctctcgctc gacggtttgc
      481 cccgcgaact gctcaccgag atcctcgaca ccgccgactc cttcctggaa gtcggtgccc
      541 gggccgtgaa gaaagtcccg ttgctgcgcg gcaagaccgt gtgcaacgtg ttcttcgaga
      601 actcgacccg tacccgcacc accttcgaac tggcggccca gcgcctgtcg gccgacgtga
      661 tcagcctgaa cgtgtcgacc tcctcgacca gcaagggcga gaccctgttc gacaccctgc
      721 gcaacctcga agccatggcg gccgacatgt ttgtggtacg ccattccgac tccggcgcgg
      781 cgcacttcat cgccgagcac gtgtgcccgg acgtcgccgt gatcaacggt ggtgacggcc
      841 gccacgcgca cccgacccag ggcatgctcg acatgctcac cattcgccgc cacaagggca
      901 gcttcgagaa cctctcggta gccatcgtcg gcgacatcct gcactcgcgc gtggcccgct
      961 ccgacatgct ggcgctcaaa gcgctgggct gcccggacat ccgggtgatc ggcccgaaaa
     1021 ccctgatccc gatcggcatc gagcagtacg gcgtaaaggt ctacaccgac ctcgccgaag
     1081 gcctgaagga tgtcgacgtg gtgatcatgc tgcgcctgca acgcgagcgc atggccggcg
     1141 gcctgctgcc cagcgagggc gagttctacc gcctgttcgg cctgaccacc gcgcgcctgg
     1201 cctgcgccaa gcctgacgcc atcgtcatgc acccgggccc gatcaaccgt ggtgtggaaa
     1261 tcgagtcggc ggtggccgac ggcaagcact cggtgatcct caaccaggtc acctacggca
     1321 tcgccgtgcg catggccgtg ctgtccatgg ccatgagcgg gcagaacgcg caacgtcaat
     1381 tcgaccagga gaacgcccag tga
//