From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Coprinus cinereus tryptophan synthetase (TRP1) gene, exon 10.
Date: 8 Apr 92 10:00:53 GMT
Article-I.D.: genbank.Apr.8.03.00.53.1992.16128
Distribution: bionet
Checksum: 12045     3

LOCUS       CCITRP10      310 bp ds-DNA             PLN       08-APR-1992
DEFINITION  Coprinus cinereus tryptophan synthetase (TRP1) gene, exon 10.
ACCESSION   M91661 M25225
KEYWORDS    tryptophan synthetase.
SEGMENT     10 of 10
SOURCE      C.cinereus (strain H9 X 12890/3; dikaryon stage) DNA, clone
            pCc1003.
  ORGANISM  Coprinus cinereus
            Eukaryota; Fungi; Eumycota; Basidiomycotina; Hymenomycetes;
            Agaricales; Coprinaceae.
REFERENCE   1  (bases 1 to 310)
  AUTHORS   Skrzynia,C., Binninger,D.M., Alspaugh,J.A.II. and Pukkila,P.J.
  TITLE     Molecular characterization of TRP1, a gene coding for tryptophan
            synthetase in the basidiomycete Coprinus cinereus
  JOURNAL   Gene 81, 73-82 (1989)
  STANDARD  simple staff_review
COMMENT     Draft entry and computer-readable sequence for [Gene, 81 (1989)
            73-82] kindly submitted
            by P.J.Pukkila, 14-JUN-1989.
FEATURES             Location/Qualifiers
     intron          order(M91660:100..123,1..29)
                     /number=9
                     /gene="TRP1"
     CDS             join(M29094:322..519,M63113:23..115,M91654:25..164,
                     M91655:24..120,M91656:24..607,M91657:16..58,
                     M91658:33..221,M91659:20..596,M91660:23..99,30..140)
                     /EC_number="4.2.1.20"
                     /product="tryptophan synthetase"
                     /gene="TRP1"
                     /codon_start=1
                     /translation="MDCVLTRSRGPGCDCLRCFRDRRYPKKEDTVPVLLALQAGGADI
                     IELGIPFSDPIADGPVIQEANTVALKNDIDYPTVLGQIREARQQGLTAPVLLMGYYNP
                     MLAYGEDKAIQDAAEAGANGFIMVDLPPEEAIAFRQKCAASNLSYVPLIAPSTTLKRI
                     QFLASIADSFIYVVSKMGTTGSSANVAVNEELPTILSRIREYTHVPLAVEFGVATRDQ
                     FNYVADAGADGVVVIGSRIVNAIKAAGEGQVPQFVENYCREVSGKGEPSRVRSPGAAQ
                     RTPSQLTPNAETAKGVENILPARFGQFGGQYVPESLVDALAELEEAHKSAIEDPAFWE
                     EVRSLYTYSNRPSNLYLAENLTKEAGGANIWLKREDLNHTGSHKINNALGQILLAKRI
                     GKTRIIAETGAGQHGVATATVCAKFGLECVIYMGAEDVRRQALKLFRIEMLGGKAWVI
                     PVHSGSCTLKDAVNEAMRDWVTNLSTTHYLVGSAIGPHPFPTIVRDFQKVIGEEIKAQ
                     LKEVRGKLPDVVVACVGGGSNAIGTFYDFIPDKSVRLVGVEAGGEGIDGHKHSATLSM
                     GQPGVLHGVRTYILQDKAGQIIETHSISAGLDYPGVGPEHAWLKDSGRADYVVCTDED
                     ALRGFRMLTQKEGIIPALESSHAIWEGVKIAKSLPKDKDIVICLSGRGDKDVEQISEL
                     LPKWADKLDWHVSSNAIPSK"
BASE COUNT       91 a     63 c     74 g     82 t
ORIGIN      Unreported.
        1 aattgcgttt acgaacgttc tttccacagt gcttgtctgg ccgtggagac aaggatgttg
       61 aacaaatctc cgagctgctg cccaagtggg ccgataaact cgactggcac gtctcttcga
      121 atgccatccc ttcgaagtaa agattttagc gtcccggatt ccacatcagt cgtgatataa
      181 agaatgtaca aataccagtc ataaaagaat ttttatgttt cttaaatcca ccaatagtgt
      241 ttgagggggt cattaaaggc atgaaggaaa ggagtatatg acatggggag ggaacttgaa
      301 gtaccgactc
//