From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: E.coli tkt gene for transketolase
Date: 11 Sep 92 10:03:35 GMT
Distribution: bionet
Checksum: 29932     5

LOCUS       ECOTKT       2441 bp ds-DNA             BCT       11-SEP-1992
DEFINITION  E.coli tkt gene for transketolase
ACCESSION   X68025
KEYWORDS    tkt gene; transketolase.
SOURCE      Escherichia coli DNA.
  ORGANISM  Escherichia coli
            Prokaryotae; Gram-negative facultatively anaerobic rods;
            Enterobacteriaceae.
REFERENCE   1  (bases 1 to 2441)
  AUTHORS   Sprenger,G.A.
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
COMMENT     *source: strain=K-12;
            From EMBL    entry ECTKT;  dated 26-AUG-1992.
FEATURES             Location/Qualifiers
     CDS             154..2148
                     /EC_number="2.2.1.1"
                     /product="transketolase"
                     /gene="tkt"
                     /codon_start=1
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     SGVTPLGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMM
                     EGISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDID
                     GHDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTRE
                     QLGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEM
                     PSDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWS
                     GSKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAA
                     LMKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGV
                     ERQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAV
                     AAYEKLTAEGVKARVVSMSSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYV
                     GLNGAIVGIETFGESAPAELLFEEFGFTVDNVVAKAKELL"
BASE COUNT      583 a    642 c    645 g    571 t
ORIGIN
        1 gatccagaga tttctgaagc ggcaaaagga tgttccatgt acatgacgcg cggcttgcgg
       61 taaattgttg gcaaattttt cccggcgtag cccaaaacgc gctgtcgtca agtcgttaag
      121 ggcgtgccct tcatcatccg atctggagtc aaaatgtcct cacgtaaaga gcttgccaat
      181 gctattcgtg cgctgagcat ggacgcagta cagaaagcca aatccggtca cccgggggcc
      241 cctatgggta tggctgacat tgccgaagtc ctgtggcgtg atttcctgaa acacaacccg
      301 cagaatccgt cctgggctga ccgtgaccgc ttcgtgctgt ccaacggcca cggctccatg
      361 ctgatctaca gcctgctgca cctcaccggt tacgatctgc cgatggaaga actgaaaaac
      421 ttccgtcagc tgcactctaa aactccgggt cacccggaaa gtggggttac accgctgggt
      481 gtggaaacca ccaccggtcc gctgggtcag ggtattgcca acgcagtcgg tatggcgatt
      541 gcagaaaaaa cgctggcggc gcagtttaac cgtccgggcc acgacattgt cgaccactac
      601 acctacgcct tcatgggcga cggctgcatg atggaaggca tctcccacga agtttgctct
      661 ctggcgggta cgctgaagct gggtaaactg attgcattct acgatgacaa cggtatttct
      721 atcgatggtc acgttgaagg ctggttcacc gacgacaccg caatgcgttt cgaagcttac
      781 ggctggcacg ttattcgcga catcgacggt catgacgcgg catctatcaa acgcgcagta
      841 gaagaagcgc gcgcagtgac tgacaaacct tccctgctga tgtgcaaaac catcatcggt
      901 ttcggttccc cgaacaaagc cggtacccac gactcccacg gtgcgccgct gggcgacgct
      961 gaaattgccc tgacccgcga acaactgggc tggaaatatg cgccgttcga aatcccgtct
     1021 gaaatctatg ctcagtggga tgcgaaagaa gcaggccagg cgaaagaatc cgcatggaac
     1081 gagaaattcg ctgcttacgc gaaagcttat ccgcaggaag ccgctgaatt tacccgccgt
     1141 atgaaaggcg aaatgccgtc tgacttcgac gctaaagcga aagagttcat cgctaaactg
     1201 caggctaatc cggcgaaaat cgccagccgt aaagcgtctc agaatgctat cgaagcgttc
     1261 ggtccgctgt tgccggaatt cctcggcggt tctgctgacc tggcgccgtc taacctgacc
     1321 ctgtggtctg gttctaaagc aatcaacgaa gatgctgcgg gtaactacat ccactacggt
     1381 gttcgcgagt tcggtatgac cgcgattgct aacggtatct ccctgcacgg tggcttcctg
     1441 ccgtacacct ccaccttcct gatgttcgtg gaatacgcac gtaacgccgt acgtatggct
     1501 gcgctgatga aacagcgtca ggtgatggtt tacacccacg actccatcgg tctgggcgaa
     1561 gacgggccga ctcaccagcc ggttgagcag gtcgcttctc tgcgcgtaac cccgaacatg
     1621 tctacatggc gtccgtgtga ccaggttgaa tccgcggtcg cgtggaaata cggtgttgag
     1681 cgtcaggacg gcccgaccgc actgatcctc tcccgtcaga acctggcgca gcaggaacga
     1741 actgaagagc aactggcaaa catcgcgcgc ggtggttatg tgctgaaaga ctgcgccggt
     1801 cagccggaac tgattttcat cgctaccggt tcagaagttg aactggctgt tgctgcctac
     1861 gaaaaactga ctgccgaagg cgtgaaagcg cgcgtggtgt ccatgtcgtc taccgacgca
     1921 tttgacaagc aggatgctgc ttaccgtgaa tccgtactgc cgaaagcggt tactgcacgc
     1981 gttgctgtag aagcgggtat tgctgactac tggtacaagt atgttggcct gaacggtgct
     2041 attgtcggta ttgaaacctt cggtgaatct gctccggcag agctgctgtt tgaagagttc
     2101 ggcttcactg ttgataacgt tgttgcgaaa gcaaaagaac tgctgtaatt agcatttcgg
     2161 gtaaaaaaga tcgcttcggc gacctttttt attaccttga taatgtccgt ttgcggacca
     2221 agcaatagat aaggcgtgtt gtagatcaca aatatttata tgcaataaat atcaattatg
     2281 taatatgcat cacgatatgc gtattgacat ttgttgttat atctataact caatgttata
     2341 taagaaatta ataattcact gttttcaaaa caaccggttt ccctgctcaa ttgctttcat
     2401 taaaccgcac gagtatttta tttttaccgc cggacggtac c
//