From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Rhizobium meliloti insertion sequence ISRM4 DNA
Date: 22 Dec 92 22:57:05 GMT
Distribution: bionet
Checksum: 53977 3

LOCUS       RHMISRM4      991 bp ds-DNA             BCT       30-NOV-1992
DEFINITION  Rhizobium meliloti insertion sequence ISRM4 DNA
ACCESSION   X65471
KEYWORDS    insertion sequence ISRM4; transposase.
SOURCE      Rhizobium meliloti (strain GR4) DNA.
  ORGANISM  Rhizobium meliloti
            Prokaryotae; Gracilicutes; Scotobacteria; Rhizobiaceae.
REFERENCE   1  (bases 1 to 991)
  AUTHORS   Toro,N.
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
REFERENCE   2  (sites)
  AUTHORS   Soto,M.J., Zorzano,A., Olivares,J. and Toro,N.
  TITLE     Sequence of ISRm4 from Rhizobium meliloti strain GR4
  JOURNAL   Gene 120, 125-126 (1992)
  STANDARD  full automatic
COMMENT     *source: strain=GR4;
               From EMBL    entry RMISRM4;  dated 14-APR-1992.
FEATURES             Location/Qualifiers
     repeat_unit     12..14
                     /rpt_type=direct
     repeat_unit     15..31
                     /rpt_type=inverted
                     /note="left invert repeat 1"
     RBS             75..78
     repeat_unit     931..947
                     /rpt_type=inverted
     repeat_unit     948..950
                     /rpt_type=direct
     repeat_unit     951..967
                     /rpt_type=inverted
                     /note="left invert repeat 2"
     CDS             83..688
                     /product="transposase"
                     /codon_start=1
                     /translation="METTMAWTDFTRPQYARRALRYASDLTDREWSLIAPFMPPPRRL
                     GRPRKTDMREVVNALLYIASTGCQWRMLPKDFPPFSTVQGYFYEWRATELWNRINHHL
                     VMETRELEGRQASPTAGVIDSQSVKTTESGGIRGFDAGKKINGRKRHIIVDTLGLMVG
                     LMVHGADVQDRDGAPDLLKSIRHRWPGCFMSLPMVATRAKS"
BASE COUNT      223 a    266 c    291 g    211 t
ORIGIN      
        1 cgatctcggt cttagaggct gaccgaaaaa gagttgagtg aaatcagtga gttgtgattc
       61 cgtcggattt gcttaagatt cgatggagac cacgatggct tggactgatt tcacccgacc
      121 gcaatatgcg cgacgggcgc tgcgatatgc aagtgatctt acggaccgcg agtggtcttt
      181 aatcgcgccg ttcatgcccc cgccccgtcg gcttggcaga ccgcgcaaga ccgatatgcg
      241 tgaggtggtg aacgcgttgc tttacatcgc gtcgaccggt tgccaatggc ggatgctgcc
      301 gaaggacttc ccgccatttt ccactgtcca gggctatttc tacgaatggc gagcgaccga
      361 gctgtggaac cggatcaacc accatctcgt catggagaca cgggaattgg agggaagaca
      421 ggcctctccc acagcgggtg tgatcgatag tcagagtgtc aaaaccaccg aaagcggcgg
      481 cattcgcggc tttgatgcgg gaaagaagat caacggccgc aagcgtcata tcatcgtcga
      541 cacgcttggc ctgatggtcg gcctcatggt gcacggggct gacgttcagg atcgcgacgg
      601 agcgcccgat ctcttgaagt ccatccgtca cagatggcct ggctgcttca tgtctttgcc
      661 gatggtggct acgcgggcga aaagctgaag agccgactga agaaaatcgg caagtggaca
      721 ctcgacatca tcaagcgctc cgacaagacc aaagcgtttg agatcctacc ccgccggtgg
      781 gttgtcgagc gcacatttgc atggctgggg cgatgccgca ggctggcgaa agacttcgag
      841 aaatccatcg cttcagccca ggcctggatc acaatcgccc acatccgcat gctcacgagg
      901 cggcttgcaa gatattgcta tcgttgatca cttttcgagt ccgactctta gaggctgcct
      961 caaaaagaat tgagtgattt cagccagttg t
//