From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Homo sapiens (clone BDC-454) prointerleukin 1 beta gene, complete
Date: 21 Dec 92 20:46:59 GMT
Distribution: bionet
Checksum: 08607 5

LOCUS       HUMPROINT    1283 bp ds-DNA             PRI       20-NOV-1992
DEFINITION  Homo sapiens (clone BDC-454) prointerleukin 1 beta gene, complete
            enhancer region.
ACCESSION   L06808
KEYWORDS    enhancer; prointerleukin 1 beta.
SOURCE      Homo sapiens (library: lambda-EMBL3) DNA.
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (sites)
  AUTHORS   Clark,B.D., Collins,K.L., Gandy,M.S., Webb,A.C. and Auron,P.E.
  TITLE     Genomic sequence for human prointerleukin 1 beta: Possible
            evolution from a reverse transcribed prointerleukin 1 alpha gene
  JOURNAL   Nucleic Acids Res. 14, 7897-7914 (1986)
  STANDARD  full automatic
REFERENCE   2  (bases 1 to 1283)
  AUTHORS   Shirakawa,F., Saito,K., Bonagura,C.A., Galson,D.L., Fenton,M.J.,
            Webb,A.C. and Auron,P.E.
  TITLE     The human prointerleukin 1 beta gene requires DNA sequences both
            proximal and distal to the transcription start site for
            tissue-specific induction
  JOURNAL   Mol. Cell. Biol. (1993) In press
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     misc_feature    1..6
                     /standard_name="Xba I"
                     /note="restriction enzyme binding site"
                     /evidence="EXPERIMENTAL"
     misc_feature    1
                     /note="position is equivalent to -3757 relative to the
                     transcription start site at position 1934 reported by
                     Clark et al., Nucleic Acids Res. 14, 7897-7914 (1986)
                     accession #X04500"
                     /citation=[1]
     misc_feature    624..629
                     /standard_name="Pvu II"
                     /note="restriction enzyme binding site"
                     /evidence="EXPERIMENTAL"
     enhancer        626..1029
                     /evidence="EXPERIMENTAL"
     protein_bind    876..889
                     /bound_moiety="NF-IL6 or related C/EBP-like protein"
     protein_bind    903..914
                     /bound_moiety="NF-Beta 1"
     protein_bind    916..922
                     /bound_moiety="NF-Beta 2"
     misc_feature    1029..1034
                     /standard_name="Xho I"
                     /note="restriction enzyme binding site"
                     /evidence="EXPERIMENTAL"
BASE COUNT      376 a    283 c    304 g    320 t
ORIGIN      chromosome 2
        1 tctagaccag ggaggagaat ggaatgtccc ttggactctg catgtcccca atctgagaac
       61 ctggatccaa gagggagaag aagcccattg gagatgatgc cataaaggaa gtggaagcga
      121 tatgataaaa atcatagtgc ccattcccaa ataatcccag aagcagaagg gaaaggagag
      181 aaatatcaca aagacaggtg tgggtacaca caacattttt tcataccttt aagatcccag
      241 aggactcatg gaaatgatac agaaatgacc tcataagaac aatattagga agccagtcca
      301 agaatgagat gggaaattgg ggaaaatgtt gggggcagat tgcttagttc tgttcctaag
      361 caagagggtg accaagaagg aacagctcac tacaagacag acatcactgc atgtacacac
      421 aataatataa gaactaaccc atgattattt tgcttgtctt cttgttcaaa atgattgaaa
      481 ccaatgagat gagtgatcaa ccttgataac tggctgcgaa gcccatgatt agacacaaga
      541 tggtatcagg gcacttgctg ctttgaataa atgtcagtct cctgtcttgg aagaatgacc
      601 ctgacagggt aaagaggaac ttgcagctga gaaaggcttt agtgactcaa gagctgaata
      661 attccccaaa agctggagca tcctggcatt tccagctccc catctctgct tgttccactt
      721 ccttggggct acatcaccat ctacatcatc atcactcttc cactccctcc cttagtgcca
      781 actatgttta tagcgagata ttttctgctc attggggatc ggaaggaagt gctgtggcct
      841 gagcggtctc cttgggaaga caggatctga tacatacgtt gcacaaccta tttgacataa
      901 gaggtttcac ttcctgagat ggatgggatg gtagcagatt tgggtccagg ttacagggcc
      961 aggatgagac atggcagaac tgtggagact gttacgtcag ggggcattgc catggctcca
     1021 aaatttccct cgagcgaaac gatcaggggc tcatgcaacc tggatactag tgctgcttca
     1081 accacactgt gctattggat gagtcacttc caccctccta gccttgattt cttcgtctgc
     1141 tgttcacatt caaatagcta ttcatgtctt catctctgtg gtcccaccat atcccaccag
     1201 acaatcatta gggctcctct tagctggcag attctgaggt cctggatgct acaattggaa
     1261 gatggagaag tagaagctca agg
//