From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Homo sapien ST2p33, complete sequence.
Date: 29 Jul 92 10:03:07 GMT
Distribution: bionet
Checksum: 32887     5

LOCUS       HUMSF2P33    1428 bp ss-mRNA            PRI       29-JUL-1992
DEFINITION  Homo sapien ST2p33, complete sequence.
ACCESSION   M69040
KEYWORDS    SF2p33.
SOURCE      Homo sapiens Female cDNA to mRNA.
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 1428)
  AUTHORS   Krainer,A.R., Mayeda,A., Kozak,D. and Binns,G.
  TITLE     Functional expression of cloned human splicing factor sf2: Homology
            to RNA-binding proteins, U1 70K, and drosophila splicing regulators
  JOURNAL   Cell 66, 383-394 (1991)
  STANDARD  simple staff_entry
COMMENT     Comments: SF2p33 is an essential pre-mRNA splicing factor, which
            can also
            affect alternative 5' splice site selection in vitro by stimulating
            the
            use of proximal 5' splice sites. (Krainer, A.R., Conway, G.C., and
            Kozak, D.
            (1990) Purification and Characterization of SF2, a Human Pre-mRNA
            Splicing
            Factor. Genes Dev. 4, 1158-1171; Krainer, A.R., Conway, G.C., and
            Kozak, D.
            (1990) The Essential Pre-mRNA Splicing Factor SF2 Influences 5'
            Splice Site
            Selection by Activating Proximal Sites. Cell 62, 35-42). This
            factor is also
            known as ASF (Ge, H., and Manley, J.L. (1990) A protein Factor,
            ASF, Controls
            Alternative Splicing of SV40 Early Pre-mRNA In Vitro. Cell 62,
            25-34).
            SF2 p33 consists of two polypeptides of apparent molecular weight
            33 kd,
            approximately, although their predicted molecular weight is 27,744
            daltons.
            The two forms appear to differ by the extent of post-translational
            modification, which includes phosphorylation. SF2p33 binds RNA and
            promotes the annealing of complementary RNAs. It is required for
            assembly
            of pre-spliceosome complexes. The N-termini of the HeLa
            polypeptides are
            blocked.
FEATURES             Location/Qualifiers
     5'UTR           <1..124
     CDS             125..871
                     /codon_start=1
                     /translation="MSGGGVIRGPAGNNDCRIYVGNLPPDIRTKDIEDVFYKYGAIRD
                     IDLKNRRGGPPFAFVEFEDPRDAEDAVYGRDGYDYDGYRLRVEFPRSGRGTGRGGGGG
                     GGGGAPRGRYGPPSRRSENRVVVSGLPPSGSWQDLKDHMREAGDVCYADVYRDGTGVV
                     EFVRKEDMTYAVRKLDNTKFRSHEGETAYIRVKVDGPRSPSYGRSRSRSRSRSRSRSR
                     SNSRSRSYSPRRSRGSPRYSPRHSRSRSRT"
     3'UTR           872..1428
BASE COUNT      341 a    296 c    400 g    391 t
ORIGIN
        1 cgagctgata aaggcgccat tttggagggg ccgcgggaga cgtggtgccg ctgcgggctc
       61 gctctgccgt gcgctaggct tggtgggaag gcctgttctc gagtccgcgc ttttcgtcac
      121 cgccatgtcg ggaggtggtg tgattcgtgg ccccgcaggg aacaacgatt gccgcatcta
      181 cgtgggtaac ttacctccag acatccgaac caaggacatt gaggacgtgt tctacaaata
      241 cggcgctatc cgcgacatcg acctcaagaa tcgccgcggg ggaccgccct tcgccttcgt
      301 tgagttcgag gacccgcgag acgcggaaga cgcggtgtat ggtcgcgacg gctatgatta
      361 cgatgggtac cgtctgcggg tggagtttcc tcgaagcggc cgtggaacag gccgaggcgg
      421 cggcgggggt ggaggtggcg gagctccccg aggtcgctat ggccccccat ccaggcggtc
      481 tgaaaacaga gtggttgtct ctggactgcc tccaagtgga agttggcagg atttaaagga
      541 tcacatgcgt gaagcaggtg atgtatgtta tgctgatgtt taccgagatg gcactggtgt
      601 cgtggagttt gtacggaaag aagatatgac ctatgcagtt cgaaaactgg ataacactaa
      661 gtttagatct catgagggag aaactgccta catccgggtt aaagttgatg ggcccagaag
      721 tccaagttat ggaagatctc gatctcgaag ccgtagtcgt agcagaagcc gtagcagaag
      781 caacagcagg agtcgcagtt actccccaag gagaagcaga ggatcaccac gctattctcc
      841 ccgtcatagc agatctcgct ctcgtacata agatgattgg tgacactttt tgtagaaccc
      901 atgttgtata cagttttcct ttattcagta caatcttttc attttttaat tcaaactgtt
      961 ttgttcagaa tgggctaaag tgttgaattg cattcttgta atatcccctt gctcctaaca
     1021 tctacattcc cttcgtgtct ttgataaatt gtattttaag tgatgtcata gacaggattg
     1081 tttaaattta gttaactcca tactcttcag actgtgatat tgtgtaaatg tctatctgcc
     1141 ctggtttgtg tgaactggga tgttgggggt gtttgtggtt atcttacctg gggaagttct
     1201 tatgtttatc ttgcttttca tgtgtctttc tgtagacata tctgaagaga tggattaaga
     1261 atgctttgga ttaaggattg tggagcacat ttcaatcatt ttaggattgt caaaaggagg
     1321 attgaggagg atcagatcaa taatggaggc aatggtatga ctccaagtgc tattgtcaca
     1381 gatgaaattg gcagtattga ccttatacta aaaggcaggg gctaaaaa
//