From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Vibrio cholerae pilus biogenesis protein (tcpE and tcpF) genes
Date: 24 Jul 92 10:10:44 GMT
Distribution: bionet
Checksum: 25071     6

LOCUS       VIBTCPEFTO   2428 bp ds-DNA             BCT       24-JUL-1992
DEFINITION  Vibrio cholerae pilus biogenesis protein (tcpE and tcpF) genes
            complete cds, virulence regulator protein (toxT) gene, 5' end.
ACCESSION   M93963
KEYWORDS    pilus biogenesis protein; virulence regulator protein.
SOURCE      Vibrio cholerae (strain 0395) DNA.
  ORGANISM  Vibrio cholerae
            Prokaryotae; Facultatively anaerobic gram-negative rods;
            Vibrionaceae.
REFERENCE   1  (bases 1 to 2428)
  AUTHORS   Kaufman,M.R., Shaw,C.E., Jones,I.D. and Taylor,R.K.
  TITLE     Biogenesis and regulation of the vibrio cholerae
            toxin-coregulated-pilus: Analogies to other virulence factor
            secretory systems and localization of the toxT virulence regulator
            gene to the TCP cluster
  JOURNAL   Unpublished (1992)
  STANDARD  full staff_entry
FEATURES             Location/Qualifiers
     CDS             112..1134
                     /product="pilus biogenesis protein"
                     /gene="tcpE"
                     /codon_start=1
                     /translation="MKIISKKYRLELYSMLVDLLNDNIPLYDALNKIQNEGVGIYDKN
                     FIKSIELIKDRMKSNSSLTDALTGLIPDKEVLMINVAENSGKISSGIAAIRKNIIDAD
                     EIKSKAISSMITPSVMLIVTMVVIAGYSVKVFPTFESVLPVSRWPGVTQALYNLGFSL
                     YEGLWIKVLIFVAIFITILVFMSKNITGNFRDGFLDKLPPFNFAKHIAATEFLANMSM
                     LLESRVPFKEGLDIVDHKTTRCVSSHLQRMKANMQEGLDYKQALDTNLLDKKMLLTMA
                     VYSELPNFSDVMQKLAIEANINLHKKIATLAGVMKNISLITLALSVIWIFGAIFSLVD
                     KLSSSL"
     CDS             1144..2160
                     /product="pilus biogenesis protein"
                     /gene="tcpF"
                     /codon_start=1
                     /translation="MRYKKTLMLSIMITSFNSFAFNDNYSSTSTVYATSNEATDSRGS
                     EHLRYPYLECIKIGMSRDYLENCVKVSFPTSQDMFYDAYPSTESDGAKTRTKEDFSAR
                     LLAGDYDSLQKLYIDFYLAQTTFDWEIPTRDQIETLVNYANEGKLSTALNQEYITGRF
                     LTKENGRYDIVNVGGVPDNTPVKLPAIVSKRGLMGTTSVVNAIPNEIYPHIKVYEGTL
                     SRLKPGGAMIAVLEYDVNELSKHGYTNLWDVQFKVLVGVPHAETGVIYDPVYEETVKP
                     YQPSNNLTGKKLYNVSTNDMHNGYKWSNTMFSNSNYKTQILLTKGDGSGVKLYSKAYS
                     ENFK"
     CDS             2363..2428
                     /gene="toxT"
                     /codon_start=1
                     /translation="MIGKKSFQTNVYRMSKFDTYIF"
                     /partial
BASE COUNT      808 a    375 c    454 g    791 t
ORIGIN
        1 gcttcagcat taaagataaa gcttattaca atgttttaaa aggcaaagtg tgtcacgaat
       61 attttatgct gcatttcggt cagttagatc atgaggatga aaatataata tatgaaaatt
      121 atctccaaga agtataggct agaactttat tcaatgcttg tggacttatt gaatgataat
      181 atcccactct atgatgcatt aaataaaata caaaatgagg gagtgggcat ctatgataag
      241 aattttatta aatccattga attaattaag gataggatga aaagtaattc atcactaacg
      301 gatgccttaa ctggactaat accagataaa gaagttttga tgattaatgt tgcagaaaac
      361 tcaggtaaga tttcgagtgg tattgctgca atacgcaaaa acattatcga tgcagatgag
      421 atcaaatcta aagctatctc atctatgatc acgcctagcg tgatgcttat cgtgactatg
      481 gttgttattg ctggctattc agtaaaagta tttcccacat ttgagtctgt acttcctgtg
      541 agccggtggc ccggtgttac ccaagcactt tataaccttg gattttcatt gtatgaaggc
      601 ttgtggatca aagttcttat ttttgtcgct atcttcatta cgattttagt ttttatgtct
      661 aaaaacatta cagggaattt cagagatggt tttttagata agctgccacc ttttaatttt
      721 gcaaagcata ttgcagcaac agaatttttg gccaatatgt ccatgttgct agagagtcga
      781 gtcccattta aagaaggtct ggacattgtc gatcataaaa cgacacgctg cgtctcctca
      841 catttgcaaa gaatgaaagc taacatgcaa gaagggctag actacaaaca ggctctcgac
      901 accaatttac tagataaaaa aatgctcctg acaatggctg tttattcaga actacctaat
      961 ttttcggatg taatgcaaaa attagctatt gaagccaata taaatctaca taaaaaaatt
     1021 gccactttag caggggtaat gaaaaatatt tcactaataa cgttggcgtt gtctgtgatt
     1081 tggatcttcg gtgcgatctt ttcgctcgtt gataaactaa gttcatcttt atagtgagga
     1141 tatatgagat ataaaaaaac cttaatgtta tcaatcatga ttacatcatt taactcattt
     1201 gcatttaatg ataattatag ttcaaccagt acggtttatg ctacgtctaa tgaagctacg
     1261 gattcaaggg ggagtgagca ccttagatac ccttatttag agtgtatcaa aatcggtatg
     1321 agtagagatt atcttgaaaa ctgtgtgaaa gtatcttttc caacctctca agatatgttt
     1381 tatgacgcat acccatcgac agaatctgac ggtgctaaga caagaacaaa agaagatttc
     1441 tctgctcggc ttttagctgg tgattatgat agtttacaga aattgtatat agatttttat
     1501 cttgctcaaa cgactttcga ttgggaaata ccgacaaggg atcaaattga gacactagtt
     1561 aattatgcta atgaaggtaa attgtctacg gcacttaatc aagagtatat tacaggtcga
     1621 tttcttacaa aagaaaatgg tcgatatgat attgtgaatg ttggtggtgt tccagataat
     1681 actccagtta aactaccagc tattgtttct aaacgcggac taatgggtac aacttctgta
     1741 gttaatgcaa ttcccaatga aatttatcct catatcaaag tttatgaagg gactcttagt
     1801 cgtttaaaac ctggtggtgc aatgatcgca gtattagaat atgacgtaaa tgaattaagt
     1861 aagcacgggt ataccaatct gtgggatgtg cagtttaagg ttcttgttgg agttccacat
     1921 gcagaaacag gagttatcta tgaccctgtt tatgaagaaa ctgtcaaacc atatcagcct
     1981 agtaacaact taacgggtaa gaagttgtat aatgtatcta caaatgacat gcataatggt
     2041 tacaagtggt caaatactat gttctcaaat tctaattata aaacgcaaat attattaact
     2101 aaaggagatg gaagtggtgt gaaactgtat agcaaagcat attcagagaa ctttaaataa
     2161 aatagataag atcacagatt cgtggctgtt atcttatctc aaaaaacata aaataacatg
     2221 agttacttta tgtttttctt atgtaatacg tctgtaactt gttcttatgt ctgttatgta
     2281 gttttatatt aacttcgatt ttaagataac tttacgtgga tggctctctg cgtttattta
     2341 tatatcttca gagtagaacg caatgattgg gaaaaaatct tttcaaacta atgtatatag
     2401 aatgagtaaa tttgatactt acatattc
//