From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: A. utahensis aculeacin A acylase gene, complete cds.
Date: 29 Dec 92 00:49:56 GMT
Distribution: bionet
Checksum: 19251 7

LOCUS       ANNAAC       2958 bp ds-DNA             BCT       04-DEC-1992
DEFINITION  A. utahensis aculeacin A acylase gene, complete cds.
ACCESSION   D10610 D90543
KEYWORDS    aculeacin A acylase.
SOURCE      Actinoplanes utahensis (strain NRRL12052) (library: cosmid) DNA.
  ORGANISM  Actinoplanes utahensis
            Prokaryotae; Firmicutes; Endospore-forming rods and cocci;
            Actinoplanetes.
REFERENCE   1  (bases 1 to 2958)
  AUTHORS   Inokoshi,J., Takeshima,H., Ikeda,H. and Omura,S.
  TITLE     Cloning and sequencing of the aculeacin A acylase-encoding gene
            from Actinoplanes utahensis and expression in Streptomyces lividans
  JOURNAL   Gene 119, 29-35 (1992)
  STANDARD  full automatic
COMMENT     Submitted (05-Nov-1991) to DDBJ by: Junji Inokoshi The Kitasato
            Institute Shirokane 5-9-1 Minato-ku Tokyo 108 Japan Phone:
            03-3444-6161 Fax:    03-3444-6637.
FEATURES             Location/Qualifiers
     RBS             109..115
     sig_peptide     121..222
                     /codon_start=1
     mat_peptide     223..2478
                     /product="aculeacin A acylase"
                     /codon_start=1
     mat_peptide     223..762
                     /product="aculeacin A acylase small subunit"
                     /codon_start=1
     mat_peptide     808..2478
                     /product="aculeacin A acylase large subunit"
                     /codon_start=1
     repeat_region   2558..2585
                     /function="transcriptional terminator"
                     /rpt_type=inverted
                     /rpt_unit=2558..2569
                     /rpt_unit=2574..2585
     CDS             121..2481
                     /gene="aac"
                     /product="aculeacin A acylase"
                     /codon_start=1
                     /translation="MTSSYMRLKAAAIAFGVIVATAAVPSPASGREHDGGYAALIRRA
                     SYGVPHITADDFGSLGFGVGYVQAEDNICVIAESVVTANGERSRWFGATGPDDADVRT
                     TSSTQAIDDRVAERLLEGPRDGVRAPCDDVRDQMRGFVAGYNHFLRRTGVHRLTDPAC
                     RGKAWVRPLSEIDLWRTSWDSMVRAGSGALLDGIVAATPPTAAGPASAPEAPDAAAIA
                     AALDGTSAGIGSNAYGLGAQATVNGSGMVLANPHFPWQGAERFYRMHLKVPGRYDVEG
                     AALIGDPIIEIGHNRTVAWSHTVSTARRFVWHRLSLVPGDPTSYYVDGRPERMRARTV
                     TVQTGSGPVSRTFHDTRYGPVAVVPGTFDWTPATAYAITDVNAGNNRAFDGWLRMGQA
                     KDVRALKAVLDRHQFLPWVNVIAADARGEALYGDHSVVPRVTGALAAACIPAPFQPLY
                     ASSGQAVLDGSRSDCALGADPDAAVPGILGPASLPVRFRDDYVTNSNDSHWLASPAAP
                     LEGFPRILGNERTPRSLRTRLGLDQIQQRLAGTDGLPGKGFTTARLWQVMFGNRMHGA
                     ELVRDDLVALCRRQPTATASNGAIVDLTAACTALSRFDERADLDSRGAHLFTEFLAGG
                     IRFADTFEVTDPVRTPAPFWNTTDPRVRTALADACNGSPASPSTRSVGDIHTDSRGER
                     RIPIHGGRGEAGTFNVITNPLVPGVGYPQVVHGTSFVMAVELGPHGPSGRQILTYAQS
                     TNPNSPWYADQTVLYSRKGWDTIKYTEAQIAADPNLRVYRVAQRGR"
BASE COUNT      433 a   1124 c   1014 g    387 t
ORIGIN      
        1 cctgccgcgc cccgccacct cggtgcgggc ctgaaatcga tgagcgtggt tgcttcatcg
       61 gcctgccgag cgatgagagt atgtgggcgg tagagcgagt ctcgaggggg agatgccgcc
      121 gtgacgtcct cgtacatgcg cctgaaagca gcagcgatcg ccttcggtgt gatcgtggcg
      181 accgcagccg tgccgtcacc cgcttccggc agggaacatg acggcggcta tgcggccctg
      241 atccgccggg cctcgtacgg cgtcccgcac atcaccgccg acgacttcgg gagcctcggt
      301 ttcggcgtcg ggtacgtgca ggccgaggac aacatctgcg tcatcgccga gagcgtggtg
      361 acggccaacg gtgagcggtc gcggtggttc ggtgcgaccg ggccggacga cgccgatgtg
      421 cgcacgacct cttccacgca agcgatcgac gaccgcgtcg ccgagcggct cctcgaaggg
      481 ccccgcgacg gcgtgcgggc gccgtgcgac gacgtccggg accagatgcg cggcttcgtc
      541 gccggctaca accacttcct acgccgcacc ggcgtgcacc gcctgaccga cccggcgtgc
      601 cgcggcaagg cctgggtgcg cccgctctcc gagatcgatc tctggcgtac gtcgtgggac
      661 agcatggtcc gggccggttc cggggcgctg ctcgacggca tcgtcgccgc gacgccaccg
      721 acagccgccg ggcccgcgtc agccccggag gcacccgacg ccgccgcgat cgccgccgcc
      781 ctcgacggga cgagcgcggg catcggcagc aacgcgtacg gcctcggcgc gcaggccacc
      841 gtgaacggca gcgggatggt gctggccaac ccgcacttcc cgtggcaggg cgccgaacgc
      901 ttctaccgga tgcacctcaa ggtgcccggc cgctacgacg tcgagggcgc ggcgctgatc
      961 ggcgacccga tcatcgagat cgggcacaac cgcacggtcg cctggagcca caccgtctcc
     1021 accgcccgcc ggttcgtgtg gcaccgcctg agcctcgtgc ccggcgaccc cacctcctat
     1081 tacgtcgacg gccggcccga gcggatgcgc gcccgcacgg tcacggtcca gaccggcagc
     1141 ggcccggtca gccgcacctt ccacgacacc cgctacggcc cggtggccgt ggtgccgggc
     1201 accttcgact ggacgccggc caccgcgtac gccatcaccg acgtcaacgc gggcaacaac
     1261 cgcgccttcg acgggtggct gcggatgggc caggccaagg acgtccgggc gctcaaggcg
     1321 gtcctcgacc ggcaccagtt cctgccctgg gtcaacgtga tcgccgccga cgcgcggggc
     1381 gaggccctct acggcgatca ttcggtcgtc ccccgggtga ccggcgcgct cgctgccgcc
     1441 tgcatcccgg cgccgttcca gccgctctac gcctccagcg gccaggcggt cctggacggt
     1501 tcccggtcgg actgcgcgct cggcgccgac cccgacgccg cggtcccggg cattctcggc
     1561 ccggcgagcc tgccggtgcg gttccgcgac gactacgtca ccaactccaa cgacagtcac
     1621 tggctggcca gcccggccgc cccgctggaa ggcttcccgc ggatcctcgg caacgaacgc
     1681 accccgcgca gcctgcgcac ccggctcggg ctggaccaga tccagcagcg cctcgccggc
     1741 acggacggtc tgcccggcaa gggcttcacc accgcccggc tctggcaggt catgttcggc
     1801 aaccggatgc acggcgccga actcgtccgc gacgacctgg tcgcgctctg ccgccgccag
     1861 ccgaccgcga ccgcctcgaa cggcgcgatc gtcgacctca ccgcggcctg cacggcgctg
     1921 tcccgcttcg atgagcgtgc cgacctggac agccggggcg cgcacctgtt caccgagttc
     1981 ctcgcgggcg gaatcaggtt cgccgacacc ttcgaggtga ccgatccggt acgcaccccc
     2041 gcgccgttct ggaacaccac ggatccgcgg gtacggacgg cgctcgccga cgcgtgcaac
     2101 ggctcgccgg catccccctc gacgcgaagc gtgggagaca tccacaccga cagccgcggc
     2161 gaacggcgca tccccatcca cggtggccgc ggggaagcag gcaccttcaa cgtgatcacc
     2221 aacccgctcg tgccgggcgt gggatacccg caggtcgtcc acggaacatc gttcgtgatg
     2281 gccgtcgaac tcggcccgca cggcccgtcg ggacggcaga tcctcaccta tgcgcagtcg
     2341 acgaacccga actcaccctg gtacgccgac cagaccgtgc tctactcgcg gaagggctgg
     2401 gacaccatca agtacaccga ggcgcagatc gcggccgacc cgaacctgcg cgtctaccgg
     2461 gtggcacagc ggggacgctg acccacgtca cgccggctcg gcccgtgcgg gggcgcaggg
     2521 cgccgatcgt ctctgcatcg ccggtcagcc ggggcctgcg tcgaccggcg gcggccggtc
     2581 gacgcccgcg tcccggcgca gcgactggct gaagcgccag gcgtcggcgg cccggggcag
     2641 gttgttgaac atcacgtacg ccgggccgcc gtcgaggatg ccggcgaggt gtgccagctc
     2701 ggcatccgtg tacacatgcc gggcgccggt gatgccgtgc agccggtaat aggccatcgg
     2761 cgtcagatcg cggcgcagga acgggtcggc ggcgtgggtc aggtccagct cctggcacaa
     2821 gccctcgacc acctcgtcgc ccacgggcgc gcgggtccca caacagccgg acaccggccg
     2881 gccggccgct cgggcgcaga actcacgcag tcgcgcgatg gcgggttcgg tcggccggaa
     2941 actcgccggg cactgcag
//