From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Plasmid ColV3-K30 (from E.coli) replication protein A (repA) gene,
Date: 10 Sep 92 10:31:38 GMT
Distribution: bionet
Checksum: 03081     4

LOCUS       CLAREPAA     1525 bp ds-DNA             BCT       10-SEP-1992
DEFINITION  Plasmid ColV3-K30 (from E.coli) replication protein A (repA) gene,
            complete cds.
ACCESSION   L01251
KEYWORDS    repA gene; replication protein A.
SOURCE      Plasmid ColV3-K30 DNA.
  ORGANISM  Plasmid ColV3-K30
            Prokaryotae.
REFERENCE   1  (bases 1 to 1525)
  AUTHORS   Gibbs,M.D., Spiers,A.J. and Bergquist,P.L.
  TITLE     RepFIB: A basic replicon of large plasmids
  JOURNAL   Unpublished (1992)
  STANDARD  full staff_entry
FEATURES             Location/Qualifiers
     CDS             276..1253
                     /note="central protein required for replication"
                     /product="replication protein A"
                     /gene="repA"
                     /codon_start=1
                     /translation="MDKSSGELVTLTPNNNNTVQPVALMRLGVFVPTLKSLKNSKKNT
                     LSRTDATEELTRLSLARAEGFDKVEITGPRLDMDNDFKTWVGIIHSFARHNVIGDKVE
                     LPFVEFAKLCGIPSSQSSRRLRERISPSLKRIAGTVISFSRTDEKHTREYITHLVQSA
                     YYDTERDIVQLQADPRLFELYQFDRKVLLQLKAINALKRRESAQALYTFIESLPRDPA
                     PISLARLRARLNLKSPVFSQNQTVRRAMEQLREIGYLDYTEIQRGRTKFFCIHYRRPR
                     LKAPNDESKENPLQPSPAEKVSPEMAEKLALLEKLGITLDDLEKLFKSR"
BASE COUNT      389 a    379 c    367 g    390 t
ORIGIN
        1 gggccgggac cacgatcaca taagctgtgg catgttactg ataaactgta acaagctaat
       61 gataagctgt attcagtaat ccatatactg aagtaagtta atgacataaa ctatggtcag
      121 tatgccagac tcagttgtta aatacaggct gcaggttttt cttcagtcag ttagcggagt
      181 tctgacacac gattttctgt ttattctttt actgtccaca ggctggaggc tttctggaaa
      241 acgaaaattc agacatcaaa aaactgttcg gcgaggtgga taagtcctcc ggtgagctgg
      301 tgacactgac accaaacaat aacaacaccg tacaacctgt ggcgctgatg cgtctgggcg
      361 tttttgtacc gacccttaaa tcactgaaga acagtaaaaa aaatacactg tcacgtactg
      421 atgccacgga agagctgaca cgtctttccc tggcccgtgc tgagggattc gataaggttg
      481 agatcaccgg cccccgcctg gatatggata atgatttcaa gacctgggtg gggatcattc
      541 attcctttgc ccgccataac gtgattggtg acaaagttga actgcctttt gttgagtttg
      601 caaaactgtg tggtatacct tcaagccagt catcccgcag gctgcgtgag cgcatcagcc
      661 cttccctgaa gcgcattgcc ggtaccgtga tttcgttttc ccgtaccgat gagaagcaca
      721 cccgggaata tatcacccat ctggtacagt cagcctacta cgatactgag cgggatattg
      781 tgcagttaca ggccgatccc cgcctgtttg aactgtacca gtttgacaga aaagtccttc
      841 tccagcttaa ggcgattaat gccctgaagc gacgggagtc cgcccaggca ctctacacct
      901 ttatagagag cctgccccgg gatccggcac cgatatcgct ggcgcggctg cgtgcacgcc
      961 tcaatctgaa gtctcctgta ttttcccaga accagacggt cagacgggca atggagcagt
     1021 tgcgcgagat tggatatctt gattacacgg agatccagcg ggggcggaca aaattcttct
     1081 gtattcacta ccggcgtccc cggttaaaag cgccgaatga cgagagtaag gaaaatccgt
     1141 tgcaaccttc acctgcggaa aaagtcagtc cggagatggc ggagaagctt gccctgcttg
     1201 agaaactggg catcacgctg gatgacctgg aaaaactctt caaatcccgc tgaacataaa
     1261 ctgtagtcag tgaagagcgc tcctttactg actacagctt atattaaccg gtgcagtgag
     1321 tggtctgctc agtgcagttt atattctgtt tcctgcagtg ctgtctgtag ctgagctgcc
     1381 atctgcctgt cccttacgta agtgatcccg taacctgatg ctgaggcatt gctcccttca
     1441 taaaacatga cttactcact acagcttata tacatgctcc agcttatgtt atgtctgttc
     1501 tgctgaccac agcttatata aggaa
//