From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Yeast (S.cerevisiae) GST1 protein gene (required for G1-to-S phase
Date: 21 Jul 92 10:09:06 GMT
Distribution: bionet
Checksum: 32305     6

LOCUS       YSCGST1      2458 bp ds-DNA             PLN       21-JUL-1992
DEFINITION  Yeast (S.cerevisiae) GST1 protein gene (required for G1-to-S phase
            transition), complete cds.
ACCESSION   Y00859 Y00829
KEYWORDS    GTP-binding protein; elongation factor 1-alpha.
SOURCE      Yeast (S.cerevisiae) DNA, clone pYK80L.
  ORGANISM  Saccharomyces cerevisiae
            Eukaryota; Fungi; Eumycota; Hemiascomycetes; Endomycetales;
            Saccharomycetaceae.
REFERENCE   1  (bases 1 to 2458)
  AUTHORS   Kukuchi,Y., Shimatake,H. and Kikuchi,A.
  TITLE     A Yeast gene required for the Gl-to-S transition encodes a protein
            containing a A-kinase target site and GTPase domain
  JOURNAL   EMBO J. 7, 1175-1182 (1988)
  STANDARD  simple staff_entry
COMMENT     Predicted protein contains a target site for cAMP-dependent protein
            kinase(s) and a GTPase domain with homology to EF1(alpha)
            Data kindly reviewed (02-May-1988) by A.Kikuchi.
            Submitted (25-MAR-1988) to the EMBL data library by A.Kikuchi.
FEATURES             Location/Qualifiers
     CDS             262..2319
                     /note="GST1 protein (put.)"
                     /note="putative"
                     /codon_start=1
                     /translation="MSDSNQGNNQQNYQQYSQNGNQQQGNNRYQGYQAYNAQAQPAGG
                     YYQNYQGYCGYQQGGYQQYNPDAGYQQQYNPQGGYQQYNPQGGYQQQFNPQGGRGNYK
                     NFNYNNNLQGYQAGFQPQSQGMSLNDFQKQQKQAAPKPKKTLKLVSSSGIKLANATKK
                     VGTKPAESDKKEEEKSAETKEPTKEPTKVEEPVKKEEKPVQTEEKTEEKSELPKVEDL
                     KISESTHNTNNANVTSADALIKEQEEEVDDEVVNDMFGGKDHVSLIFMGHVDAGKSTM
                     GGNLLYLTGSVDKRTIEKYEREAKDAGRQGWYLSWVMDTNKEERNDGKTIEVGKAYFE
                     TEKRRYTILDAPGHKMYVSEMIGGASQADVGVLVISARKGEYETGFERGGQTREHALL
                     AKTQGVNKMVVVVNKMDDPTVNWSKERYDQCVSNVSNFLRAIGYNIKTDVVFMPVSGY
                     SGANLKDHVDPKECPWYTGPTLLEYLDTMNHVDRHINAPFMLPIAAKMKDLGTIVEGK
                     IESGHIKKGQSTLLMPNKTAVEIQNIYNETENEVDMAMCGEQVKLRIKGVEEEDISPG
                     FVLTSPKNPIKSVTKFVAQIAIVELKSIIAAGFSCVMHVHTAIEEVHIVKLLHKLEKG
                     TNRKSKKPPAFAKKGMKVIAVLETEAPVCVETYQDYPQLGRFTLRDQGTTIAIGKIVK
                     IAE"
BASE COUNT      844 a    469 c    503 g    642 t
ORIGIN      Map position = chrIVR.
        1 gatcatacag aagttattgt cacttcttac cttgctctta aatgtacatt acaaccgggt
       61 attatatctt acatcatcgt ataatatgat ctttctttat ggagaaaatt tttttttcac
      121 tcgaccaaag ctcccattgc ttctgaagag tgtagtgtat attggtacat cttctcttga
      181 aagactccat tgtactgtaa caaaaagcgg tttcttcatc gacttgctcg gaataacatc
      241 tatatctgcc cactagcaac aatgtcggat tcaaaccaag gcaacaatca gcaaaactac
      301 cagcaataca gccagaacgg taaccaacaa caaggtaaca acagatacca aggttatcaa
      361 gcttacaatg ctcaagccca acctgcaggt gggtactacc aaaattacca aggttattgt
      421 gggtaccaac aaggtggcta tcaacagtac aatcccgacg ccggttacca gcaacagtat
      481 aatcctcaag gaggctatca acagtacaat cctcaaggcg gttatcagca gcaattcaat
      541 ccacaaggtg gccgtggaaa ttacaaaaac ttcaactaca ataacaattt gcaaggatat
      601 caagctggtt tccaaccaca gtctcaaggt atgtctttga acgactttca aaagcaacaa
      661 aagcaggccg ctcccaaacc aaagaagact ttgaagcttg tctccagttc cggtatcaag
      721 ttggccaatg ctaccaagaa ggttggcaca aaacctgccg aatctgataa gaaagaggaa
      781 gagaagtctg ctgaaaccaa agaaccaact aaagagccaa caaaggtcga agaaccagtt
      841 aaaaaggagg agaaaccagt ccagactgaa gaaaagacgg aggaaaaatc ggaacttcca
      901 aaggtagaag accttaaaat ctctgaatca acacataata ccaacaatgc caatgttacc
      961 agtgctgatg ccttgatcaa ggaacaggaa gaagaagtgg atgacgaagt tgttaacgat
     1021 atgtttggtg gtaaagatca cgtttcttta attttcatgg gtcatgttga tgccggtaaa
     1081 tctactatgg gtggtaatct actatacttg actggctctg tggataagag aactattgag
     1141 aaatatgaaa gagaagccaa ggatgcaggc agacaaggtt ggtacttgtc atgggtcatg
     1201 gataccaaca aagaagaaag aaatgatggt aagactatcg aagttggtaa ggcctacttt
     1261 gaaactgaaa aaaggcgtta taccatattg gatgctcctg gtcataaaat gtacgtttcc
     1321 gagatgatcg gtggtgcttc tcaagctgat gttggtgttt tggtcatttc cgccagaaag
     1381 ggtgagtacg aaaccggttt tgagagaggt ggtcaaactc gtgaacacgc cctattggcc
     1441 aagacccaag gtgttaataa gatggttgtc gtcgtaaata agatggatga cccaaccgtt
     1501 aactggtcta aggaacgtta cgaccaatgt gtgagtaatg tcagcaattt cttgagagca
     1561 attggttaca acattaagac agacgttgta tttatgccag tatccggcta cagtggtgca
     1621 aatttgaaag atcacgtaga tccaaaagaa tgcccatggt acaccggccc aactctgtta
     1681 gaatatctgg atacaatgaa ccacgtcgac cgtcacatca atgctccatt catgttgcct
     1741 attgccgcta agatgaagga tctaggtacc atcgttgaag gtaaaattga atccggtcat
     1801 atcaaaaagg gtcaatccac cctactgatg cctaacaaaa ccgctgtgga aattcaaaat
     1861 atttacaacg aaactgaaaa tgaagttgat atggctatgt gtggtgagca agttaaacta
     1921 agaatcaaag gtgttgaaga agaagacatt tcaccaggtt ttgtactaac atcgccaaag
     1981 aaccctatca agagtgttac caagtttgta gctcaaattg ctattgtaga attaaaatct
     2041 atcatagcag ccggtttttc atgtgttatg catgttcata cagcaattga agaggtacat
     2101 attgttaagt tattgcacaa attagaaaag ggtaccaacc gtaagtcaaa gaaaccacct
     2161 gcttttgcta agaagggtat gaaggtcatc gctgttttag aaactgaagc tccagtttgt
     2221 gtggaaactt accaagatta ccctcaatta ggtagattca ctttgagaga tcaaggtacc
     2281 acaatagcaa ttggtaaaat tgttaaaatt gccgagtaaa tttcttgcaa acataagtaa
     2341 atgcaaacac aataataccg atcataaagc attttcttct atattaaaaa acaaggttta
     2401 ataaagctgt tatatatata tatatatata tagacgtata attagtttag ttcttttt
//