From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Synechococcus sp. IsiA protein (isiA) and flavodoxin (isiB) genes,
Date: 29 Dec 92 20:58:54 GMT
Distribution: bionet
Checksum: 00545 6

LOCUS       SYOISIA      2220 bp ds-DNA             BCT       11-DEC-1992
DEFINITION  Synechococcus sp. IsiA protein (isiA) and flavodoxin (isiB) genes,
            complete cds.
ACCESSION   M88253
KEYWORDS    IsiA protein; flavodoxin.
SOURCE      Synechococcus sp. (library: M13) DNA.
  ORGANISM  Synechococcus sp.
            Prokaryotae; Gracilicutes; Oxyphotobacteria; Cyanobacteria;
            Chroococcales.
REFERENCE   1  (bases 1 to 2220)
  AUTHORS   Leonhardt,K.G. and Straus,N.A.
  TITLE     An iron stress operon involved in photosynthetic electron transport
            in the marine cyanobacterium Synechococcus sp. PCC 7002
  JOURNAL   J. Gen. Microbiol. 138, 1613-1621 (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     protein_bind    84..98
                     /bound_moiety="fur repressor"
                     /note="putative"
     protein_bind    131..145
                     /bound_moiety="fur repressor"
                     /note="putative"
     -35_signal      277..282
     protein_bind    284..298
                     /bound_moiety="fur repressor"
                     /note="putative"
     -10_signal      303..308
     protein_bind    318..332
                     /bound_moiety="fur repressor"
                     /note="putative"
     RBS             378..381
     terminator      1471..1500
                     /gene="isiA"
     terminator      2115..2145
                     /gene="isiB"
     CDS             388..1416
                     /gene="isiA"
                     /product="IsiA protein"
                     /codon_start=1
                     /translation="MQTYDNPDVKYEWWAGNARFADLSGQFIGAHVAHAALIVFWAGA
                     FTLFEISYFDPTLPMGEQNLILLPHLATLGLGIEGNGAINTEPYFVIGAIHLISSAVL
                     AAGGLFHVLRGPQDLKTATGPARRFHFDWEDPKQLGLILGHHLLLLGLGAFLLVAKAM
                     YFGGLYDTATQTVRLVTEPTLDPAVIYGYQTHFATVDNLEDIVGGHIYVGVLLVAGGI
                     WHILVPPLQWAKKVLLFSGEAILSYSLGAIALAGFVAAYFCAVNTTAYPVEFYGPVLD
                     VKLSIVPYFADTIELPMNEHTSRAWLANAHFFFAFFFLQGHLWHALRAMGFDFRRVEK
                     VLSDPLDA"
     CDS             1583..2095
                     /gene="isiB"
                     /product="flavodoxin"
                     /codon_start=1
                     /translation="MSKIGLFFGTQTGNTEELAQAIQAAFGGSDIVELFDVAEVDIEA
                     LRDFDQLIIGCPTWNVGELQSDWEALYDDLDDVDFSGKTIAYFGAGDQVGYADNFQDA
                     MGVLEEKITSLGGKTVGQWPTAGYDHSESKAERDGKFVGLAIDEDNQPELTAERIQAW
                     VAQLKPAFGL"
BASE COUNT      553 a    500 c    521 g    646 t
ORIGIN      
        1 gacttagtta atttagcgta gtttgcggcc aggaatccga ctgataccta ggcgatcgcc
       61 ttttattgca tgaacaccaa ctgataatta atagtgataa aacttgtctg aaataattat
      121 tgggtttctt catatcaaaa ctgattagca aaaaagacgt gatcgttgcg gcgagaagtg
      181 gagcgagaaa ttcgtaaaaa ctcctatttt ctttgcagag ataaggtttt tggtctcatg
      241 gagtgattgg ccagggcaac tttttacaaa agttaacttg aactttactg agaataattg
      301 taggataaag ccaatccttt attgccaaat atatgcgata agtgattagc ctgattagtt
      361 ttaacgacca ctctttttaa aatcacgatg caaacctacg ataatccaga cgttaaatac
      421 gaatggtggg caggcaatgc ccggtttgct gacctttccg gtcagtttat tggtgcccac
      481 gtggcccatg ctgccttaat tgttttctgg gcaggggcct tcacgttgtt cgaaatttct
      541 tactttgacc cgaccctacc catgggcgag caaaatttaa tcctcttgcc ccatttagcc
      601 acccttggcc tgggcatcga aggaaacggt gcgatcaata cagagcctta ttttgtgatt
      661 ggtgcaattc acctaatttc ttcagcggta ctggcggcag ggggattgtt ccatgtgttg
      721 cgtggccccc aagatctcaa aacagccacc ggcccagccc gtcgtttcca ttttgattgg
      781 gaagacccga agcaattggg tttaattttg ggccatcact tactgttgct gggactgggt
      841 gctttcttgc tcgtagcgaa agcgatgtac tttggcggtt tgtacgacac agcgacccaa
      901 accgtgcgct tggttacaga gccgaccctc gatccggcgg tgatctacgg gtatcaaacc
      961 cacttcgcca ccgttgataa tctcgaagat atcgttggtg gccatattta tgtgggtgta
     1021 ctgcttgtgg ctgggggaat ttggcacatt ctcgtgccgc cactccagtg ggcaaaaaag
     1081 gtgctcctgt tctctgggga agcgattttg tcctattccc tgggggcgat cgccttggcg
     1141 ggatttgtgg ccgcctactt ctgcgcagtg aataccacgg cctatcccgt tgaattttat
     1201 gggcctgttc tcgatgtcaa actcagcatt gtgccttact ttgccgacac catcgagctc
     1261 cccatgaacg aacacacttc ccgtgcctgg ttagccaatg cccatttctt ctttgccttt
     1321 ttcttcctcc aggggcacct ctggcacgcc ctccgcgcca tgggctttga tttccgtcga
     1381 gtcgaaaaag tccttagcga tccactggat gcctaggttt tttaaccgct acacatcaac
     1441 ttagtaaaag ttttcctctc atagacaatc gggttccatg cagtgatttg gaaccttttt
     1501 tctttctcct aacgcgactc tttttagagt aattgatttt tcaattcaaa caactttcta
     1561 aagcaagatt acggttttaa tcatgtcaaa aattggttta tttttcggca cacaaacagg
     1621 caacacagaa gaacttgccc aagcgatcca agcggccttt ggtggttcag atattgtcga
     1681 actttttgat gtggctgagg ttgatatcga agcactacgg gattttgatc agctgatcat
     1741 tggttgtcca acctggaatg tgggagaact acagtcagac tgggaagccc tctatgatga
     1801 tctcgatgac gtagactttt caggaaaaac cattgcctat tttggggctg gggatcaagt
     1861 gggctatgcc gacaattttc aggatgccat gggcgtttta gaagaaaaaa ttaccagcct
     1921 tggcgggaaa accgtaggcc aatggcccac cgctggttat gaccacagcg aatccaaagc
     1981 ggaacgggac ggtaaatttg tcggtttggc gatcgatgaa gataatcaac cggaattgac
     2041 ggcagaacgt attcaagctt gggttgccca acttaaacca gcctttggtc tgtagaacgc
     2101 ttgattaatg tcattcaggg gaggagcttg ttgtactctt cctctggtgg aatcaaaagg
     2161 aattcggtaa agggattttt ccaaataaat tttttttaat caataacaac aataaaaaat
//