From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: P.boryanum nifU gene for NifU protein, nifH for dinitrogenase
Date: 13 Aug 92 10:01:05 GMT
Distribution: bionet
Checksum: 12274     8

LOCUS       PEENIFUHD    3409 bp ds-DNA             BCT       13-AUG-1992
DEFINITION  P.boryanum nifU gene for NifU protein, nifH for dinitrogenase
            reductase and nifD for dinitrogenase alpha subunit.
ACCESSION   D00666
KEYWORDS    dinitrogenase; dinitrogenase reductase; nifD; nifH; nifU;
            nitrogenase.
SOURCE      P.boryanum (strain M101) DNA.
  ORGANISM  Plectonema boryanum
            Prokaryotae; Gracilicutes; Oxyphotobacteria; Cyanobacteria;
            Nostocales; Scytonemataceae.
REFERENCE   1  (bases 1 to 3409)
  AUTHORS   Fujita,Y., Takahashi,Y., Shonai,F., Ogura,Y. and Matsubara,H.
  TITLE     Cloning, nucleotide sequences and differential expression of the
            nifH and nifH-like (frxC) genes from the filamentous
            nitrogen-fixing cyanobacterium Plectonema boryanum
  JOURNAL   Plant Cell Physiol. 32, 1093-1106 (1991)
  STANDARD  full staff_review
COMMENT     These data kindly submitted in computer readable form by:
            Yuichi Fujita
            Department of Biology
            Faculty of Science, Osaka Univ.
            1-1 Machikaneyama
            Toyonaka, Osaka 560
            Japan
            Phone:  06-844-1151 x4296
            Fax:    06-855-8139
FEATURES             Location/Qualifiers
     CDS             <1..620
                     /note="NifU protein"
                     /codon_start=3
                     /translation="ALNVSNQDIAAFLGGLPEAKMHCSVMGQEALEAAIFKYRGIEVE
                     HHEEDEGALICSCFGISEPKIRRVVIENGLTTVEQVTSYVKAGGGCGSCLADIEDIIT
                     AVIDEKETTAARIAAEISEAQIRKPLTNVQKIALIQKVLDEEVRPVLIADGGDVELYD
                     VDGDFVKVTLKGACGSCASSTATLKDAVEAKLRLRVLPTLVVQAV"
     repeat_unit     708..733
                     /note="inverted repeat 1"
     RBS             787..792
                     /note="ribosome binding site for nifH"
     CDS             802..1692
                     /note="nitrogenase reductase (nifH)"
                     /codon_start=1
                     /translation="MSDENIRQIAFYGKGGIGKSTTSQNTIAALAEMGERIMIVGCDP
                     KADSTRLMLHSKAQTTILHLAAERGAVEDLELEEVLLTGYRDVKCVESGGPEPGVGCA
                     GRGIITAINFLEENGAYEDLDFVSYDVLGDVVCGGFAMPIREGKAQEIYIVTSGEMMA
                     MYAANNIARGILKYAHSGGVRLGGLICNSRKVDREIELIETLAQRLNTQMIHFVPRDN
                     IVQHAELRRMTVNEYAPDSAQAQEYATLARKIKDNTNLTIPTPISMDELEDLLVEFGL
                     LGGDEEYEKAIKQDLAKRGA"
     repeat_unit     1705..1734
                     /note="inverted repeat 2"
     RBS             1806..1808
                     /note="ribosome binding site for nifD"
     CDS             1817..3301
                     /note="alpha-subunit of dinitrogenase (nifD)"
                     /codon_start=1
                     /translation="MTPPESVTLEENAATAVDPKELIKDVLEAYPEKGRKKREKHLNV
                     YQEGKPDCGVKSNIKSLPGSMTTRGCAYAGSKGVVWGPIKDMIHISHGPVGCGYYSWS
                     GRRNYYIGTTGIDTFGTMQFTSDFQERDIVFGGDKKLAKLITELEELFPLNRGISIQS
                     ECPIGLIGDDIEAVAKKSAKEINKAVVPVRCEGFRGVSQSLGHHIANDAVRDWIFPRT
                     DKAKKDGTIDVDPTQYDVAIIGDYNIGGDAWSSRILLEEIGLRVVAQWSGDGTINELI
                     NTPTVKLNLVHCYRSMNYISRHMEETYGIPWLEYNFFGPTQIAKSLREIAAKFDETIQ
                     AKAEEVIAKYQAQTDEVIAKFLPRLQGKTVALMVGGLRPRHVVPAFYDLGMRLIGTGY
                     EFGHNDDYKRTTHYIEDGTLIYDDVSAFEFEEFIKEMKPDLVASGIKEKYVFQKMALP
                     FRQMHSWDYSGPYHGYDGFAIFARDMDMALNSPTWSLIGAPWSK"
     repeat_unit     3339..3356
                     /note="inverted repeat 3"
BASE COUNT      949 a    827 c    817 g    816 t
ORIGIN
        1 aagctttgaa tgtcagtaat caagatattg cagcattctt aggtggactc ccagaggcta
       61 aaatgcattg ttctgttatg ggacaagaag cattagaagc tgccatcttc aaatatcgag
      121 gcatcgaagt cgaacaccac gaagaagatg aaggcgcatt gatttgtagt tgctttggca
      181 ttagcgaacc gaagattcgt cgagtcgtaa ttgagaacgg actgacgaca gtagaacagg
      241 tcacaagcta tgtcaaagca ggcggcggct gtggttcttg tcttgcagat attgaagata
      301 tcatcactgc ggtgatcgat gagaaagaaa ccactgctgc cagaattgca gctgaaatct
      361 ccgaggcaca gatccgtaaa cctctgacca atgttcagaa aattgcactg attcaaaaag
      421 tgctagacga agaagttcga cccgtcttga ttgcagatgg cggcgatgtt gaactttacg
      481 atgtcgatgg cgatttcgtc aaagtaacgc tcaaaggagc ctgtggttct tgtgcaagca
      541 gtaccgccac gctcaaagat gccgtcgaag ctaagttgcg tcttcgagtc cttcccaccc
      601 ttgttgtcca agcggtgtaa cgttcctacc taatgtccta atgaatttaa aaaaatctct
      661 gccctggggc gaacgatcgc cacctccgta aacgagagag tgagagcttt cgattcaaca
      721 gttttgattg gaacgtctct accccccacc gacaacctga attgacaaac cacacaccta
      781 attcaaagga gatccattct catgtctgac gaaaatatta gacagatagc gttctacggc
      841 aaaggcggta tcggtaaatc caccacttcg cagaacacga tcgcagcttt agcagaaatg
      901 ggcgaacgca tcatgattgt aggatgcgac ccaaaagcag attccacccg cttgatgttg
      961 cacagtaaag cacaaaccac cattctgcac ttagcagcag aacgcggcgc agttgaagac
     1021 ctcgaactcg aagaagtgct attgactggc tatcgcgatg tgaagtgcgt tgagtccggc
     1081 ggcccagaac ccggtgtcgg ttgtgcaggc cgaggcatca tcaccgcaat caacttcctt
     1141 gaagaaaacg gtgcttatga agatctcgat ttcgtatcgt atgacgtact aggtgacgtt
     1201 gtatgcggcg gtttcgcaat gccaattcgc gaaggtaaag cacaagaaat ctacattgtg
     1261 acttccggtg aaatgatggc aatgtatgct gcaaacaaca ttgcacgagg cattctgaag
     1321 tatgctcact ccggtggtgt ccgtttgggc ggtctgatct gtaacagccg taaggtcgat
     1381 cgagaaatcg aattgatcga aaccttggca caacgtctga atactcaaat gattcacttt
     1441 gtccctcgcg acaacatcgt ccaacacgca gaactgcgcc ggatgaccgt gaacgagtac
     1501 gctcctgaca gcgctcaagc acaagaatac gcaaccctag ctcgcaagat caaagacaac
     1561 acgaatctca cgattccgac tccgatctcg atggacgaat tagaagacct cttggtcgaa
     1621 ttcggtctgt taggcggcga cgaagagtac gaaaaagcga tcaagcaaga cctagcgaaa
     1681 cgtggcgcgt aggacttaga agtaggggaa ttggggaaac ttcccctact ccccactccc
     1741 tcactccccc acctccccac tccctactct ctctcaccca ttccacaacc ttcagcactc
     1801 tcagcgaggc acacctatga ctcctccgga atctgtaacc ctagaagaaa atgcagcaac
     1861 tgctgttgac cccaaagaac taatcaaaga cgttctagag gcataccctg aaaaaggacg
     1921 caaaaaacgg gaaaaacacc tcaacgtcta ccaagaaggc aaacccgatt gcggcgttaa
     1981 atccaacatc aaatccttac ctggttccat gaccacccgt gggtgtgcct atgcaggctc
     2041 gaagggtgtg gtttggggtc ccattaagga catgattcac attagccatg gcccagttgg
     2101 ttgcggctac tattcctggt ctggtcgtcg gaactactac attggtacaa ccggaattga
     2161 tactttcgga accatgcaat ttacctccga cttccaagaa cgcgacatcg tattcggtgg
     2221 tgacaaaaag ctggcaaaac tgatcacaga actcgaagaa ctgtttcctc tcaatcgcgg
     2281 gatttccatt caatctgaat gtccaattgg tttgattgga gatgatattg aagcggttgc
     2341 taagaaatcc gcaaaagaaa ttaataaagc agtggttcca gttcgatgcg aaggcttccg
     2401 aggcgtttct caatccttgg gtcaccacat tgcaaacgat gcggtgcggg actggatttt
     2461 ccccagaacc gataaagcga aaaaagatgg cacgatcgat gttgatccga ctcaatatga
     2521 tgttgccatc attggggact acaacattgg tggagatgct tggtcaagcc ggattctgct
     2581 ggaagaaatt ggcttacgcg tcgttgcgca atggtcgggt gatggcacca tcaacgagtt
     2641 gatcaatacg ccgactgtga agttaaatct ggttcactgt tatcgatcga tgaactacat
     2701 cagtcgtcac atggaagaaa cgtatggtat tccctggttg gagtacaact tcttcgggcc
     2761 gactcagatt gcaaaatcgt tgcgcgaaat tgcggctaag tttgacgaaa ccattcaagc
     2821 caaagcagaa gaagtgatcg cgaaatacca agcccaaacg gacgaagtca tcgcgaagtt
     2881 ccttcctcgt ttacaaggta aaacagttgc tctgatggtc ggcggtctgc gtcctcgtca
     2941 cgttgttcct gctttctatg atctcggaat gagattgatc ggaactggat atgagtttgg
     3001 tcacaacgat gactacaaac gcacgacgca ctatatcgaa gatggcacac tgatctatga
     3061 tgacgtttct gcctttgagt ttgaggaatt catcaaggaa atgaagcctg acttggttgc
     3121 ttctggaatt aaagagaagt atgtcttcca aaagatggca cttcctttcc gtcaaatgca
     3181 ctcctgggac tactctggcc catatcacgg ttacgatggt ttcgcgatct tcgctcgcga
     3241 tatggatatg gcgctgaata gtccaacctg gagcttaatc ggcgctccct ggagcaaata
     3301 gtcttttgct gcgtttggag caacttctgc cccctaaatc ccccatgtat gggggacttt
     3361 gagaacagtc actgattcga tttcgtgagc acgcttcgtg agcaagctt
//