From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Tomato BiP (binding protein)/grp78 (glucose-regulated protein, 78
Date: 27 Jan 93 06:02:36 GMT
Distribution: bionet
Checksum: 64479 8

LOCUS       TOMBIPGRP    2966 bp ds-DNA             PLN       26-JAN-1993
DEFINITION  Tomato BiP (binding protein)/grp78 (glucose-regulated protein, 78
            kD) (BiP/grp78) gene, 5'end.
ACCESSION   L08829
KEYWORDS    binding protein; glucose-regulated protein 78;
            heat shock protein 70; homologue.
SOURCE      Lycopersicon esculentum (cultivar VFNT Cherry) (library: Charon 35
            from J. Deikman and R.L. Fischer) DNA.
  ORGANISM  Lycopersicon esculentum
            Eukaryota; Plantae; Embryobionta; Magnoliophyta; Magnoliopsida;
            Asteridae; Solanales; Solanaceae.
REFERENCE   1  (bases 1 to 2966)
  AUTHORS   Meyer,D.J., Ewing,N.N., Rosichan,J.L. and Bennett,A.B.
  TITLE     Characterization of the tomato endoplasmic reticulum-localized
            BiP/GRP78 homolog
  JOURNAL   Unpublished (1993)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     mat_peptide     join(1468..1709,1800..2014,2104..2346,2625..>2966)
                     /function="molecular chaperon"
                     /standard_name="BiP/grp78"
                     /product="glucose-regulated protein 78"
                     /gene="BiP/grp78"
                     /codon_start=1
     sig_peptide     join(256..313,1442..1467)
                     /gene="BiP/grp78"
                     /evidence="EXPERIMENTAL"
                     /codon_start=1
     exon            one-of(137..313,168..313)
                     /number=1
                     /gene="BiP/grp78"
     5'UTR           one-of(137..255,168..255)
                     /gene="BiP/grp78"
                     /evidence="EXPERIMENTAL"
                     /note="S1 nuclease protection and primer extension"
     prim_transcript one-of(137..>2966,168..>2966)
                     /gene="BiP/grp78"
     intron          314..1441
                     /number=1
                     /gene="BiP/grp78"
     exon            1442..1709
                     /number=2
                     /gene="BiP/grp78"
     intron          1710..1799
                     /number=2
                     /gene="BiP/grp78"
     exon            1800..2014
                     /number=3
                     /gene="BiP/grp78"
     intron          2015..2103
                     /number=3
                     /gene="BiP/grp78"
     exon            2104..2346
                     /number=4
                     /gene="BiP/grp78"
     intron          2347..2624
                     /number=4
                     /gene="BiP/grp78"
     exon            2625..>2966
                     /number=5
                     /gene="BiP/grp78"
     CDS             join(256..313,1442..1709,1800..2014,2104..2346,
                     2625..>2966)
                     /function="molecular chaperon"
                     /gene="BiP/grp78"
                     /note="an endoplasmic reticulum residing hsp70 family
                     member"
                     /product="glucose-regulated protein 78"
                     /codon_start=1
                     /translation="MAACSRRGNSLVVLAIVLLGCLSALSNAKEEATKLGTVIGIDLG
                     TTYSCVGVYKNGHVEIIANDQGNRITPSWVAFTDNERLIGEAAKNLAAVNPERTIFDV
                     KRLIGRKFEDKEVQRDMKLVPYKIVSKDGKPYIQVKIKDGEVKVFSPEEISAMILTKM
                     KETAEAFLGKTIKDAVVTVPAYFNDAQRQATKDAGVIAGLNVARIINEPTAAAIAYGL
                     DKKGGEKNILVFDLGGGTFDVSILTIDNGVFEVLATNGDTHLGGEDFDQRIMEYFIKL
                     IKKKHGKDISKDNRALGKLRREAERAKRSLSSQHQVRVEIESLFDGTDFSEPLTRARF
                     EELNNDLFRKTMGPVKKAMDDAGLQKNQIDEIVLVGGSTRI"
BASE COUNT      802 a    491 c    654 g   1019 t
ORIGIN      
        1 gaattcaccc tcctcttcct attggcccac gtcatttaac atgacgtgtc taatgtcact
       61 ctttgaacgg agacccctcg ggtctttgtt cagtttctca cagtataaaa agctcccgat
      121 cacctcgcga ggctctatat atataaatct ctcatcattt ccagttcttc ataactgaaa
      181 aagataaaca gagggatcac attgtgttgt acctgtagct tagtgtattc tacgttgatt
      241 acgaaggaag aagctatggc tgcctgttcg aggcgaggca attctctcgt cgtgctagcg
      301 atcgttttat taggtaaact ttctgtttct atttgctcgt agatctgttt tgattattgt
      361 tttttgaatt ttttctggtt tatgagattt gatttgttat gttatgatct agaatgtagt
      421 agatcctaga tctgttagct tttgtttata atgtatgcgt tttcattgta gatttgtagg
      481 tttgatgtgc taattttaga tctagcgata gttttcacac aatttcacat ctgtttgagg
      541 atttgtatgg tgttttgtgt gattgatatg gcttgttttg atttcctcga tcgtgtttaa
      601 tggagaaatg atttagcact ggaatagaat agtcgatgca tgattcatgt ggtgcgccac
      661 aggagaattt caaatgctcg tttatgaagt ggaaataacg ctgtatctga tatgaaatta
      721 gtggttaggt gctttatcat ttataaatat aagcaaacat ttattagatt ctcactgcta
      781 ttttctcctg ttcttgtttt tgattgaaga attacatcat ctaatcagat gttgataacc
      841 gtggtgtgtg tatattatct atttatagtc ccatggcaaa ggtagttggt tcacatgcac
      901 ccgtgcatct gtccgtgtat gtattctagt ttctactcta gatcagattt taactccaga
      961 cagagaaaca aggtcactgc tacactggca ttgcatatat cctgaacctt ttagacattt
     1021 atcgtcgtcc tctttttttt ttccgggtga caagcctttt aatttgtact tgctagaatg
     1081 ctaataattt gggatgcatg tttggactgt ggttctggat gatgtacatt tagaaggatc
     1141 gttttcagat cctatggata cttgaaaacc cttttattta gctgactgat cgtctgaata
     1201 tgcgaaaagt tgctggtttt tgttcttagc ttatctatgt tctatgaccc tttttttatt
     1261 tctactgaat agcctgtatg ttgacttgag ttgagtggaa taggtacgaa tgatttatat
     1321 gactatgagt tccatacgtg catacatata ttccttttgc tgtgttatgt catgaatttc
     1381 atattgtact tccagagtct gatgagggtc atctgttatc ttactctttt ccttgaaaca
     1441 ggctgtcttt ctgcactttc caatgcaaag gaggaggcta caaaacttgg taccgttata
     1501 ggaattgatc ttgggacaac ctattcctgt gttggggtgt acaagaatgg tcatgtggag
     1561 attatagcta atgaccaagg aaacaggatt acaccatcat gggttgcatt cactgataac
     1621 gagagattga ttggtgaggc tgccaagaac ctggctgcgg tcaacccaga aagaacaatc
     1681 tttgatgtta agaggctgat tggaagaaag taatggacaa ttctcaatta gtttcacatg
     1741 ttttgttatg attgtatatc tgtcatgcta agagatgctt tatcttcttt ttttttcaga
     1801 tttgaagata aggaagtaca gagggatatg aagctggtac cgtacaagat tgtcagcaag
     1861 gatggaaagc cctacataca agtcaaaatc aaggatggag aagttaaggt atttagtcct
     1921 gaggaaatca gcgccatgat tttgacaaag atgaaagaga cagccgaagc attccttggg
     1981 aagactatta aggatgctgt ggtcactgtt cctggtatgt tgttatcagg tcccaaatgt
     2041 tcaatttttt tgttttcatt tttcttttct gtcattattt agagacttgt ctcgtctctg
     2101 cagcatattt caatgatgca caaagacagg cgacaaagga tgctggtgtt attgctggat
     2161 taaatgttgc taggatcatt aatgagccaa cagctgcagc cattgcctac ggactggata
     2221 agaaaggtgg tgagaagaac attcttgtct ttgaccttgg tggtgggaca ttcgatgtca
     2281 gtattctgac cattgacaat ggtgtatttg aggttcttgc aacaaatggt gacacccatc
     2341 ttggaggtaa ggatctatgg tatagaaaca acttaaagtt gagcatgaca cattttgata
     2401 ctgctaacca taatgctgtt agggtgtcct gattgtattt tctaatggac ttctcctcaa
     2461 cttgatatca actaatgaaa tgctaaaaca cttttctatt gattgcacat tagtggggtt
     2521 gctcttttgg gagggtattt tatgatagct gaggaagatt cagatgccat cctatgagat
     2581 agttattctt agattatatt ttgaatatcc gatacatctt gcaggtgagg actttgatca
     2641 aaggattatg gaatacttca tcaagttgat caagaagaag catggaaagg acatcagcaa
     2701 ggacaacaga gctcttggaa agctaaggag agaagctgag cgtgccaaga gatcccttag
     2761 tagccagcac caagtccggg ttgagattga atcattattt gatggtactg atttctctga
     2821 accactgacc cgtgctcgct ttgaggagtt gaacaatgat ctcttcagaa agacaatggg
     2881 acctgtgaag aaggcaatgg atgatgctgg cctacaaaag aaccagattg atgagattgt
     2941 tcttgttggt ggaagcacca gaattc
//