From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Rhodospirillum rubrum hydrogenase (cooH) gene, 3'end, iron sulfur
Date: 1 Sep 92 10:03:21 GMT
Distribution: bionet
Checksum: 28864     9

LOCUS       RSPCOOHFS    3701 bp ds-DNA             BCT       01-SEP-1992
DEFINITION  Rhodospirillum rubrum hydrogenase (cooH) gene, 3'end, iron sulfur
            protein (cooF), and carbon monoxide dehydrogenase (cooS) genes,
            complete cds.
ACCESSION   M90421
KEYWORDS    carbon monoxide dehydrogenase; cooF gene; cooH gene; cooS gene;
            hydrogenase; iron sulfur protein.
SOURCE      Rhodospirillum rubrum (strain UR1) DNA.
  ORGANISM  Rhodospirillum rubrum
            Prokaryotae; Gracilicutes; Anoxyphotobacteria;
            Purple nonsulfur bacteria.
REFERENCE   1  (bases 1 to 3701)
  AUTHORS   Kerby,R.L., Hong,S.S., ensign,S.A., Coppoc,L.J., Ludden,P.W. and
            Roberts,G.P.
  TITLE     Genetic and physiological characterization of the Rhodospirillum
            rubrum carbon monoxide dehydrogenase system
  JOURNAL   J. Bacteriol. 174, 5284-5294 (1992)
  STANDARD  simple staff_entry
FEATURES             Location/Qualifiers
     CDS             1..567
                     /function="required for the oxidation of carbon monoxide"
                     /product="hydrogenase"
                     /gene="cooH"
                     /codon_start=1
                     /translation="KLEPTLRDEIIPLYQTNPSIVDRTRGIGVLSAADCVDYGLMGPV
                     ARGSGHAYDVRKQAPYAVYDRLDFEMALGEHGDVWSRAMVRWQEALTSIGLIRQCLRD
                     MPDGPTKAGPVPPIPAGEAVAKTEAPRGELIYYLKTNGTDRPERLKWRVPTYMNWDAL
                     NVMMAGARISDIPLIVNSIDPCISCTER"
                     /partial
     RBS             1004..1008
                     /gene="cooF"
     CDS             1018..1590
                     /function="required for the oxidation of carbon monoxide"
                     /product="iron sulfur protein"
                     /gene="cooF"
                     /codon_start=1
                     /translation="MPPIKENVIIYANPDHCLSCHSCELACAVAHSGGHDMIEAIAAN
                     LPLHARNKVVSVDGTAMPMQCRQCEDAPCTFACPTGACRQADGQVQIVEQHCIGCKLC
                     VMVCPFGAITVRSETVVEQGACTNRGVAKKCDLCVDWRASTGKTAPACVEACPTKAIR
                     MVDLDAYRIALREARAREIAKSHRHMRVQF"
     RBS             1596..1600
                     /gene="cooS"
     CDS             1610..3529
                     /function="required for the oxidation of carbon monoxide"
                     /product="carbon monoxide dehydrogenase"
                     /gene="cooS"
                     /codon_start=1
                     /translation="MTHHDCAHCSSDACATEMLNLAEANSIETAWHRYEKQQPQCGFG
                     SAGLCCRICLKGPCRIDPFGEGPKYGVCGADRDTIVARHLVRMIAAGTAAHSEHGRHI
                     ALAMQHISQGELHDYSIRDEAKLYAIAKTLGVATEGRGLLAIVGDLAAITLGDFQNQD
                     YDKPCAWLAASLTPRRVKRLGDLGLLPHNIDASVAQTMSRTHVGCDADPTNLILGGLR
                     VAMADLDGSMLATELSDALFGTPQPVVSAANLGVMKRGAVNIAVNGHNPMLSDIICDV
                     AADLRDEAIAAGAAEGINIIGICCTGHEVMMRHGVPLATNYLSQELPILTGALEAMVV
                     DVQCIMPSLPRIAECFHTQIITTDKHNKISGATHVPFDEHKAVETAKTIIRMAIAAFG
                     RRDPNRVAIPAFKQKSIVGFSAEAVVAALAKVNADDPLKPLVDNVVNGNIQGIVLFVG
                     CNTTKVQQDSAYVDLAKSLAKRNVLVLATGCAAGAFAKAGLMTSEATTQYAGEGLKGV
                     LSAIGTAAGLGGPLPLVMHMGSCVDNSRAVALATALANKLGVDLSDLPLVASAPECMS
                     EKALAIGSWAVTIGLPTHVGSVPPVIGSQIVTKLVTETAKDLVGGYFIVDTDPKSAGD
                     KLYAAIQERRAGLGL"
     RBS             3588..3591
                     /note="unidentified ORF"
     CDS             3597..3701
                     /note="unidentified ORF"
                     /codon_start=1
                     /translation="MKIAVTGKGGVGKSTIVGMLARALSDEGWRVMAID"
                     /partial
     misc_binding    3615..3680
                     /bound_moiety="ATP"
BASE COUNT      673 a   1156 c   1155 g    717 t
ORIGIN
        1 aagcttgaac caacgctgcg cgacgagatc atcccgcttt atcagaccaa cccgtcgatc
       61 gttgatcgca cccggggcat tggcgttctg agcgcggccg attgcgtgga ctatggcctg
      121 atggggccgg tggcgcgcgg ctcgggccat gcctatgacg tgcgcaagca ggcgccctat
      181 gcggtttatg atcgtctgga cttcgagatg gccctgggcg agcacggcga tgtgtggtcg
      241 cgcgccatgg tgcgctggca agaggccctg acctcgatcg ggctgatccg ccaatgcctg
      301 cgcgacatgc ccgacggtcc gacgaaggcg gggccggtgc cgcccattcc cgctggcgag
      361 gcggtcgcca agaccgaggc gccgcgcggc gagttgatct attacctgaa gaccaacggc
      421 accgaccggc ccgaacgcct gaaatggcgc gttcccacct atatgaactg ggatgcgctc
      481 aatgtgatga tggccggggc gcggatttcc gatatcccgc tgatcgtcaa cagcatcgat
      541 ccctgcatct cctgtacgga acgctgaacg ccccagacag agaagcgttc tcatcttcta
      601 gttctgctgg tttttcttga taaatccccg cgatggcgat gaacgcttcg tggggatttt
      661 gccgagtcct tcggcgtctt ttcatacccc cataaaaact ctggataact gtcatctggc
      721 cgacagacgg ggccgggctt tttgtcgctt actcggcgcc aggggcaaaa ggcccggccc
      781 ggtctgaagg gcgacgaagc acccgccggc cgtggttttt gatcggcgaa gtctttgttt
      841 cgtctcgttt tttaaggaaa ccccgaacag gggtggcgtt aattcacgag atgactttgt
      901 aaattacgcg tctgagaaca gcgtttcgct ccagggaatg attgttgcag acagtgcagt
      961 ctaaaaaggc aaatgcgttg ttttgacgtg gtaatcaatg cgtggagggc agtacgaatg
     1021 ccgccaatca aagaaaatgt aatcatctac gccaatcccg atcattgtct gtcctgccat
     1081 tcatgcgagt tggcttgcgc cgtcgcccat agtggcggtc acgacatgat cgaggcgatt
     1141 gccgccaatt tgccgctgca tgcgcgcaac aaggtggtca gcgtcgatgg cacggcgatg
     1201 cccatgcagt gccggcagtg cgaggatgcg ccctgcacct tcgcctgccc gaccggcgcc
     1261 tgtcgtcagg ccgatggtca ggttcagatc gtcgagcagc actgcatcgg ttgcaagctc
     1321 tgcgtgatgg tctgcccctt cggggcgatc accgtgcgct cggaaacggt cgtcgagcag
     1381 ggcgcctgca ccaaccgggg cgtcgccaag aaatgcgatc tgtgtgtcga ctggcgcgcc
     1441 agcaccggca agacggctcc ggcctgtgtc gaggcttgcc cgaccaaggc catccgcatg
     1501 gtcgatctcg acgcctatcg gatcgccttg cgcgaggccc gggcccgcga gatcgccaag
     1561 tcgcaccgcc atatgcgcgt ccaattctaa gcaccaagga atcgcagtga tgacccatca
     1621 cgactgcgcc cactgttcct ccgacgcctg cgcgaccgaa atgttgaatt tggccgaggc
     1681 caacagcatc gaaaccgctt ggcatcgcta cgaaaagcag cagccccaat gcggcttcgg
     1741 cagcgccggc ctgtgttgcc gcatctgcct gaagggcccc tgtcgcatcg atccctttgg
     1801 cgaaggcccg aagtacggcg tctgcggcgc cgaccgcgac accatcgtcg cccgccatct
     1861 ggtgcgcatg atcgccgccg gcaccgccgc ccattccgag cacggccgcc acatcgccct
     1921 ggccatgcag catatttcac aaggcgagct tcacgattat tcgatccgcg atgaggccaa
     1981 gctctatgcc atcgccaaga ccctgggcgt ggccaccgag ggccgtggtc tgttggcgat
     2041 cgtgggcgat ttggcggcga tcaccctggg tgacttccag aaccaggatt acgacaaacc
     2101 ttgcgcttgg cttgccgcgt cgctgacgcc gcgtcgggtg aagcgcttgg gtgatctggg
     2161 actgctgccc cataatatcg atgcttcggt ggcgcagacg atgtcgcgca cccatgtggg
     2221 ctgcgatgcc gatcccacca atctgatcct gggcggcttg cgcgtcgcca tggccgatct
     2281 tgacggttcg atgctggcga ccgagctgtc cgatgccctg tttggcacgc cccagccggt
     2341 ggtcagtgcc gccaatctgg gcgtcatgaa gcgcggggcc gtcaatatcg ccgtcaacgg
     2401 ccataacccg atgctcagcg acatcatttg cgatgtggcg gccgatctgc gcgacgaggc
     2461 gatcgccgcc ggcgcggccg agggcatcaa catcatcggc atctgctgta ccggccatga
     2521 ggtgatgatg cgccacggcg tgccgctggc cactaactac ctgtcgcagg aattgccgat
     2581 cctgaccggc gccctggagg cgatggtcgt cgatgtccag tgcatcatgc cgtcgctgcc
     2641 gcgcatcgcc gaatgcttcc acacccagat catcaccacc gacaaacaca acaagatcag
     2701 tggcgccacc catgtgccct tcgacgagca caaggcggtg gagaccgcca agacgattat
     2761 ccgcatggcg atcgccgcct ttggccggcg cgatcccaac cgcgtcgcca ttcccgcctt
     2821 caagcagaag tcgatcgtcg gcttcagcgc cgaagccgtc gtcgccgccc tggccaaggt
     2881 caatgcggac gatccgctga agccgctggt cgataatgtg gtcaacggca atatccaggg
     2941 tatcgtgctg ttcgtcggct gcaacacgac caaggttcag caagacagcg cctatgtcga
     3001 tctcgccaaa tccctggcca agcgcaatgt gctggtgctg gcgacgggct gcgccgccgg
     3061 cgccttcgcc aaggccggat tgatgacctc cgaggcgacc acccaatacg ctggcgaggg
     3121 gttgaagggg gtgctgagcg ccatcggcac cgccgccggc ctgggggggc cgctgccctt
     3181 ggtcatgcat atgggctctt gcgtcgataa cagccgggcg gtggctttgg ccacggcttt
     3241 ggccaataag ctgggcgtcg atctctcgga tctgccgctg gtcgccagcg cgccggaatg
     3301 catgagcgag aaggccctgg ccatcggctc gtgggcggtg accatcggtc tgccgaccca
     3361 tgtcggctcg gtgcctccgg tcatcggttc gcagatcgtg accaagctgg tgaccgaaac
     3421 ggccaaggac ttggtgggcg gttatttcat cgttgatacc gaccccaaaa gcgccgggga
     3481 caagctttac gccgccattc aagagcgtcg cgccgggctt ggcctgtaag accttaacga
     3541 ccccggggcg gggaagttga tcctcgcccc ctctctttct tatcggtgag ggtgccatga
     3601 agatcgcggt gacgggaaag ggcggggttg gcaagtcgac catcgttggc atgctggccc
     3661 gcgccttgtc cgacgagggg tggagggtga tggccatcga t
//