From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Homo sapiens 1,4-alpha-glucan branching enzyme (HGBE) mRNA,
Date: 31 Dec 92 01:37:21 GMT
Distribution: bionet
Checksum: 22061 7

LOCUS       HUMHGBE      2955 bp ss-mRNA            PRI       17-DEC-1992
DEFINITION  Homo sapiens 1,4-alpha-glucan branching enzyme (HGBE) mRNA,
            complete cds.
ACCESSION   L07956
KEYWORDS    1,4-alpha-glucan branching enzyme.
SOURCE      Homo sapiens liver cDNA to mRNA.
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chorqata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 2955)
  AUTHORS   Thon,V.J., Khalil,M. and Cannon,J.F.
  TITLE     Isolation of human glycogen branching enzyme cDNAs by screening
            complementation in yeast
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     misc_feature    1..12
                     /evidence="EXPERIMENTAL"
                     /citation=[1]
                     /note="this is the 5' adaptor sequence used to clone cDNAs
                     in this library"
     misc_feature    2900..2955
                     /evidence="EXPERIMENTAL"
                     /citation=[1]
                     /note="this is the 3' adaptor used in the cDNA library
                     construction; the 16 a's should be considered the true 3'
                     end, although in vivo length may actually be longer"
     CDS             91..2199
                     /function="branch glycogen"
                     /EC_number="2.4.1.18"
                     /standard_name="glycogen branching enzyme"
                     /gene="HGBE"
                     /evidence="EXPERIMENTAL"
                     /citation=[1]
                     /product="1,4-alpha-glucan branching enzyme"
                     /codon_start=1
                     /translation="MAAPMTPAARPEDYEAALNAALADVPELARLLEIDPYLKPYAVD
                     FQRRYKQFSQILKNIGENEGGIDKFSRGYESFGVHRCADGGLYSKEWAPGAEGVFLTG
                     DFNGWNPFSYPYKKLDYGKWELYIPPKQNKSVLVPHGSKLKVVITSKSGEILYRISPW
                     AKYVVREGDNVNYDWIHWDPEHSYEFKHSRPKKPRSLRIYESHVGISSHEGKVASYKH
                     FTCNVLPRIKGLGYNCIQLMAIMEHAYYASFGYQITSFFAASSRYGTPEELQELVDTA
                     HSMGIIVLLDVVHSHASKNSADGLNMFDGTDSCYFHSGPRGTHDLWDSRLFAYSSWEV
                     LRFLLSNIRWWLEEYRFDGFRFDGVTSMLYHHHGVGQGFSGDYSEYFGLQVDEDALTY
                     LMLANHLVHTLCPDSITIAEDVSGMPALCSPISQGGGGFDYRLAMAIPDKWIQLLKEF
                     KDEDWNMGDIVYTLTNRRYLEKCIAYAESHDQALVGDKSLAFWLMDAEMYTNMSVLTP
                     FTPVIDRGIQLHKMIRLITHGLGGEGYLNFMGNEFGHPEWLDFPRKGNNESYHYARRQ
                     FHLTDDDLLRYKFLNNFDRDMNRLEERYGWLAAPQAYVSEKHEGNKIIAFERAGLLFI
                     FNFHPSKSYTDYRVGTALPGKFKIVLDSDAAEYGGHQRLDHSTDFFSEAFEHNGRPYS
                     LLVYIPSRVALILQNVDLPN"
BASE COUNT      853 a    562 c    650 g    890 t
ORIGIN      
        1 gatctgaatt cggtcccagc tagagctcca gcgcccgctc aggccccact cgaccctctc
       61 gggcctcggc tacttggact gcggcggaat atggcggctc cgatgactcc cgcggctcgg
      121 cccgaggact acgaggcggc gctcaatgcc gccctggctg acgtgcccga actggccaga
      181 ctcctggaga tcgacccgta cttgaagccc tacgccgtgg acttccagcg caggtataag
      241 cagtttagcc aaattttgaa gaacattgga gaaaatgaag gtggtattga taagttttcc
      301 agaggctatg aatcatttgg cgtccacaga tgtgctgatg gtggtttata ctccaaagaa
      361 tgggccccgg gagcagaagg agtttttctt actggagatt ttaatggttg gaatccattt
      421 tcgtacccat acaaaaaact ggattatgga aaatgggagc tgtatatccc accaaagcag
      481 aataaatctg tactcgtgcc tcatggatcc aaattaaagg tagttattac tagtaaaagc
      541 ggagagatct tgtatcgtat ttcaccgtgg gcaaagtatg tggttcgtga aggtgataat
      601 gtgaattatg attggataca ctgggatcca gaacactcat atgagtttaa gcattccaga
      661 ccaaagaagc cacggagtct aagaatttat gaatctcatg tgggaatttc ttcccatgaa
      721 ggaaaagtag cttcttataa acattttaca tgcaatgtac taccaagaat caaaggcctt
      781 ggatacaact gcattcagtt gatggcaatc atggagcatg cttactatgc cagctttggt
      841 taccaaatca caagcttctt tgcagcttcc agccgttatg gaacacctga agagctacaa
      901 gaactggtag acacagctca ttccatgggt atcatagtcc tcttagatgt ggtacacagc
      961 catgcttcaa aaaattcagc agatggattg aatatgtttg atgggacaga ttcctgttat
     1021 tttcattctg gacctagagg gactcatgat ctttgggata gcagattgtt tgcctactcc
     1081 agctgggaag ttttaagatt ccttctgtca aacataagat ggtggttgga agaatatcgc
     1141 tttgatggat ttcgttttga tggtgttacg tccatgcttt atcatcacca tggagtgggt
     1201 caaggtttct caggtgatta cagtgaatat ttcggactac aagtagatga agatgccttg
     1261 acttacctca tgttggcaaa tcatttggtt cacacgctgt gtcccgattc tataacaata
     1321 gctgaggatg tatcaggaat gccagctctg tgctctccaa tttcccaggg agggggtggt
     1381 tttgactatc gactagccat ggcaattcca gataagtgga ttcagctact taaagagttt
     1441 aaagatgaag actggaacat gggcgatata gtatacacgc tcacaaacag gcgctacctt
     1501 gaaaagtgca ttgcttatgc agagagccat gatcaggcat tggttgggga taagtcgctg
     1561 gcattttggt tgatggatgc cgaaatgtat acaaacatga gtgtcctgac tccttttact
     1621 ccagttattg atcgtggaat acagcttcat aaaatgattc gactcattac gcatgggctt
     1681 ggtggagaag gctatctcaa tttcatgggt aatgaatttg ggcatcctga atggttagac
     1741 ttcccaagaa aaggaaataa tgagagttac cattatgcca ggcggcagtt tcatttaact
     1801 gacgacgacc ttcttcgcta caagttccta aataattttg acagggatat gaatagattg
     1861 gaagaaagat atggttggct tgcagctcca caggcctacg tgagtgaaaa acatgaaggc
     1921 aataagatca ttgcttttga aagagcaggt cttcttttca ttttcaactt ccatccaagc
     1981 aagagctaca ctgactaccg agttggaaca gcattgccag ggaaattcaa aattgtgcta
     2041 gattcagatg cagcggaata tggagggcat cagagactgg accacagcac tgactttttt
     2101 tctgaggctt ttgaacataa tgggcgtccc tattctcttt tggtgtacat tccaagcaga
     2161 gtggccctca tccttcagaa tgtggatctg ccgaattgaa gaggcctgat ttcagctcca
     2221 ccagatgcag atttgtgttt tgttttcttg ttatcactgt cacacagctt ataacatgta
     2281 tgcttttcag aatacagttg tctagccaag ccatcaagtg tctgaaattc aatattggtt
     2341 tatgcaaata cagcaaactt ttatttaagt agataggaga atatgtttaa aatattagga
     2401 atcctagacc atattttcaa gtcatcttag cagctaggat tctcaaatgg aagtgttata
     2461 tataatatgt taaaaacatt ttgctttcct ggctaattat ttgatccttt taaatccaaa
     2521 tttgaatcat ttgtcatgta tgattatttc tgttaaatgt acacagtatt taagatggat
     2581 atttggtggc tctatttgtt ctgatatctt ttggtctaaa ttatgaggta ccaagattgt
     2641 ttctttgttt ctttttttca aattgtgttt agaaatactg taataaatat gcagtagtga
     2701 tataaagaat tatatccaag gtaatataaa agccattacg tatgaactca tccgtgtctc
     2761 attttgtgtt ttattttgtg atctcttgtc cactaagtat cttgttaaat gccagtatct
     2821 cagtctttct gaagccctga aatggtaatt gtagcatttc agaaaatgtc tttcatttca
     2881 atcaataaaa agcttttgta aaaaaaaaaa aaaaaaaaaa aaaaaccgtc gacaaagcgg
     2941 ccgcaaaccg aattc
//