From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Pseudomonas putida morphine dehydrogenase (morA) gene, complete
Date: 22 Dec 92 22:56:49 GMT
Distribution: bionet
Checksum: 14206 4

LOCUS       PSEMORA      1260 bp ds-DNA             BCT       30-NOV-1992
DEFINITION  Pseudomonas putida morphine dehydrogenase (morA) gene, complete
            cds.
ACCESSION   M94775
KEYWORDS    morA gene; morphine dehydrogenase.
SOURCE      Pseudomonas putida (strain M10) DNA.
  ORGANISM  Pseudomonas putida
            Prokaryotae; Gracilicutes; Scotobacteria; Pseudomonadaceae.
REFERENCE   1  (bases 1 to 1260)
  AUTHORS   Willey,D.L., Caswell,D.A., Lowe,C.R. and Bruce,N.C.
  TITLE     Nucleotide sequence and overexpression of morphine dehydrogenase, a
            plasmid encoded gene from Pseudomonas putida M10
  JOURNAL   Biochem. J. (1992) In press
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     RBS             157..160
                     /gene="morA"
     mat_peptide     172..1053
                     /product="morphine dehydrogenase"
                     /gene="morA"
                     /codon_start=1
     terminator      1073..1099
                     /note="rho independent"
     RBS             1204..1207
                     /note="putative"
     CDS             169..1056
                     /gene="morA"
                     /note="mature peptide"
                     /product="morphine dehydrogenase"
                     /codon_start=1
                     /translation="MAGKSPLINLNNGVKMPALGLGVFAASAEETASAIASAISSGYR
                     LIDTARSYNNEAQVGEGIRNSVDRAEMFVTTKLFNCDYGYERALRAFDESLGRLGLDY
                     VDLYLLHWPTKDWNATIQSWKAAEKILGDGRARAIGVCNFLEDQLDELIAASDVVPAV
                     NQIELHPYFAQKPLLAKNRALGIVTEAWSPIGCHQRWQDGDNHGGRKHPLTDPVITTI
                     AEAHGRSAAQVILRWHFQNDVVAIPKSVNPERIAKNIDVFDFALSDAEMAQLDELDTG
                     VRIGPDPRDVDTSSFAEFV"
     CDS             1213..>1260
                     /note="putative;  ORF"
                     /codon_start=1
                     /translation="MKRQDLPRSRSLRSCE"
BASE COUNT      291 a    336 c    369 g    264 t
ORIGIN      
        1 ctacggattc tggaaaccat ggctctctta catacgagtc gtattgcagt tgacatacgt
       61 atcgcatgtc attaaggtat tttggatact cacgaatgta agtgtgacca acacagcgag
      121 tgatcgtcaa cgcaaacctg ccgtcccgta tatcgaagga gcattgttat ggccggaaaa
      181 agccctctca tcaacttgaa taatggcgtg aaaatgccgg cactaggtct cggagtgttc
      241 gctgcgtccg ccgaagagac cgcctccgcc atcgcgtcgg caatctccag tggctaccgt
      301 ctgatcgaca ccgccaggtc gtataacaat gaggctcagg tcggtgaagg catccgcaac
      361 agcgtggatc gcgccgagat gttcgtcacc acaaagctgt tcaactgcga ctatggttac
      421 gagagggcgc tcagggcatt cgacgaaagc ctcggcaggt tggggctcga ctatgtcgat
      481 ttgtatctgc tgcactggcc gaccaaagat tggaacgcca cgatccagtc ctggaaggcg
      541 gcggagaaaa ttcttggtga cgggcgtgcg cgcgcgatcg gtgtatgcaa cttcctggag
      601 gatcaactgg acgagctgat cgcggcaagt gacgtcgtgc cggcggtcaa ccagatcgag
      661 ctgcacccct atttcgcgca gaagccgctg ctggcgaaga acagggcgct cggcatcgtc
      721 acggaggctt ggtcgccgat cggctgccat caacgatggc aggatgggga caatcatggc
      781 ggcaggaagc acccgctgac cgatccggtc atcaccacta tcgccgaagc ccatggtaga
      841 tctgccgcgc aggtgatctt gcgctggcac ttccagaacg atgtcgttgc aattccgaag
      901 tcagtcaacc ccgagcgcat tgccaagaat attgacgtgt tcgatttcgc gcttagcgac
      961 gctgagatgg cgcagctaga cgagctggat acaggggtgc gcatcggccc tgatccgcgc
     1021 gacgtggaca ctagcagctt tgccgaattt gtttgatctg agatgatgcg atgaaccccg
     1081 cctatgatct cgggggttcc tgcagcaaca tttcggcaaa tcgggaaact ggtatcatcg
     1141 atcgcatgag atgacgtgct cccctgaaat tctgccagtc tgagctagag tccttcatga
     1201 aggaggacga agatgaagcg ccaggattta ccgaggagca gatcattgcg atcctgcgag
//