From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human immunodeficiency virus type 1, isolate SF13, tat, rev, vpU,
Date: 29 Dec 92 01:27:48 GMT
Distribution: bionet
Checksum: 20109 10

LOCUS       HIVSF2B13    3983 bp ss-RNA             VRL       08-DEC-1992
DEFINITION  Human immunodeficiency virus type 1, isolate SF13, tat, rev, vpU,
            env and nef coding sequences and 3'LTR.
ACCESSION   L07422
KEYWORDS    .
SOURCE      Human immunodeficiency virus type 1 (HIV-1), SF13, isolated from
            the same patient that produced SF2; cocultured with PBMCs and
            cloned into lambda phage; subclone pUC19 DNA.
  ORGANISM  Human immunodeficiency virus type 1
            Viridae; ss-RNA enveloped viruses; Positive strand RNA virus;
            Retroviridae; Lentivirinae.
REFERENCE   1  (bases 1 to 3983)
  AUTHORS   Cheng-Mayer,C., Shioda,T. and Levy,J.
  TITLE     Host range, replicative, and cytopathic properties of human
            immunodeficiency virus type 1 are determined by very few amino acid
            changes in tat and gp120
  JOURNAL   J. Virol. 65, 6930-6941 (1991)
  STANDARD  full automatic
COMMENT     The Sf13 viral isolate was recovered from the same indivicual that
            produced Sf2 (see <HIVSF2>), but five months latere when the
            individual developed KS and PCP. The increased pathogenicity and
            wider host range of SF13 is studied in depth in {1}: two aa changes
            in tat and 22 aa changes in env, going from SF2 to SF13 are of
            special significance.
FEATURES             Location/Qualifiers
     exon            91..305
                     /number=2
                     /product="tat protein"
                     /gene="tat"
     exon            230..305
                     /number=2
                     /product="rev protein"
                     /gene="rev"
     exon            2603..2904
                     /number=3
                     /product="rev protein"
                     /gene="rev"
     exon            2630..2720
                     /number=3
                     /product="tat protein"
                     /gene="tat"
     LTR             3349..3982
                     /note="3'"
     CDS             join(91..305,2630..2720)
                     /gene="tat"
                     /product="tat protein"
                     /codon_start=1
                     /translation="MEPVDPNLEPWKHPGSQPRTACNNCYCKKCCFHCYACFTRKGLG
                     ISYGRKKRRQRRRTPQDSQTDQASLSKQPASQPRGDPTGPTESKKKVERETETDPFD"
     CDS             join(230..305,2603..2904)
                     /gene="rev"
                     /product="rev protein"
                     /codon_start=1
                     /translation="MAGRSGDSDEELLRTVRLIKLLYQSTGILTIIISDPPPNPEGTR
                     QARRNRRRRWRERQRQIRSISGWILSTYLGRSAEPVPLQLPPLERLTLDCSEDCGNSG
                     TQGVGSPQIWVESPAVLDSGTKE"
     CDS             322..567
                     /gene="vpu"
                     /product="vpu protein"
                     /codon_start=1
                     /translation="MQSLQILAIVSLVVVAIIAIVVWTIVLIEYRKILRQRKIDRLFD
                     RIREKAEDSGNESERDQEELSALVEMGHLAPWDVDDL"
     CDS             485..3046
                     /gene="env"
                     /product="envelope polyprotein"
                     /codon_start=1
                     /translation="MKVKETRKNYQHLWRWGILLLGMLMICSAKEKLWVTVYYGVPVW
                     KEATTTLFCASDARAYDTEVHNVWATHACVPTDPNPQEVVLGNVTENFNMWKNNMVEQ
                     MQEDIISLWDQSLKPCVKLTPLCVTLNCTDLGKATTNSNNWKEEIKGEIKNCSFYITT
                     SIRDKIQKENALFRNLDVVPIDNASTTTNYTNYRLIHCNRSVITQACPKVSFEPIPIH
                     YCAPAGFAILKCNNKTFNGKGPCTNVSTVQCTHGIKPVVSTQLLLNGSLAEEEVVIRS
                     DNFTDNAKTIIVQLNESVAINCTRPNNNTRKGIHIGPGRAVYTTGRIIGDIRKAHCNV
                     SRTQWNNTLEQIVKKLREQFGNNKTIVFNQSSGGDPEIVMHSFNCRGEFFYCNTAQLF
                     NSTWNPNNTEGNNTDPIILPCRIKQIINMWQEVGKAMYAPPIGGQISCSSNITGLLLT
                     RDGGINGTNDTEVFRPGGGDMRDNWRSELYKYKVVKIEPLGIAPTKAKRRVVQREKRA
                     VGIIGAMFLGFLGAAGSTMGARSMTLTVQARKLLSGIVQQQNNLLRAIEAQQHLLQLT
                     VWGIKQLQARVLAVERYLRDQQLLGIWGCSGKLICTTAVPWNTSWSNKSMEDIWDNMT
                     WMQWEKEIDNYTNTIYTLLEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKIF
                     IMIVGGLVGLRIVFAVLSVVNRVRQGYSPLSFQTRLPTPRGPDRPDGIEEEGGERDRD
                     RSVRLVDGFLALIWEDLRSLCLFSYRRLRDLLLIAVRTVEILGHRGWEVLKYGWSLLQ
                     YWIQELRNSAVSLLNATAIAVAEGTDRVIEVVQRAYRAILHIPRRIRQGLERLLL"
     CDS             3048..3680
                     /gene="nef"
                     /product="nef protein"
                     /codon_start=1
                     /translation="MGGKWSKRSMGGWSAIRERMRRAEPRAEPAADGVGAVSGDLEKH
                     GAITSSNTAATNADCAWLEAQEEEEVGFPVRPQVPLRPMTYKAAVDISHFLKEKGGLE
                     GLIWSQRRQEILDLWIYHTQGYFPDWQNYTPGPGIRYPLTFGWCFKLVPLEPEQVEEA
                     NEGENNSLLHPISQHGMDDPEKEVLVWKFDSKLAFHHMARELHPEYYKDC"
BASE COUNT     1338 a    716 c    980 g    949 t
ORIGIN      
        1 gaattctgca acaactgctg tttattcatt tcagaattgg gtgtcaacat agcagaatag
       61 gcattattca acagaggaga gcaagaagaa atggagccag tagatcctaa tctagagccc
      121 tggaagcatc caggaagtca gcctaggact gcttgtaaca attgctattg taaaaagtgt
      181 tgctttcatt gctacgcgtg tttcacaaga aaaggcttag gcatctccta tggcaggaag
      241 aagcggagac agcgacgaag aactcctcag gacagtcaga ctgatcaagc ttctctatca
      301 aagcagtaag taataaatgt aatgcaatct ttacaaatat tagcaatagt atcattagta
      361 gtagtagcaa taatagcaat agttgtgtgg accatagtac tcatagaata tagaaaaata
      421 ttaagacaaa gaaaaataga caggttattt gatagaataa gagaaaaagc agaagacagt
      481 ggcaatgaaa gtgaaagaga ccaggaagaa ttatcagcac ttgtggagat ggggcatctt
      541 gctccttggg atgttgatga tctgtagtgc taaagaaaaa ttgtgggtca cagtctatta
      601 tggggtacct gtgtggaagg aagcaactac cactctattt tgtgcatcag atgctagagc
      661 atatgataca gaggtacata atgtttgggc cacacatgcc tgtgtaccca cagaccccaa
      721 cccacaagaa gtagtattgg gaaatgtgac agaaaatttt aacatgtgga aaaataacat
      781 ggtagaacag atgcaggagg atataatcag tttatgggat caaagcctaa agccatgtgt
      841 aaaattaacc ccactctgtg ttactttaaa ttgcactgat ttggggaagg ctacaaccaa
      901 tagtaataat tggaaagaag aaataaaagg agaaataaaa aactgctctt tctatatcac
      961 cacaagcata agagataaga ttcagaaaga aaatgcactt tttcgtaacc ttgatgtagt
     1021 accaatagat aatgctagta ctactaccaa ctataccaac tataggttga tacattgtaa
     1081 cagatcagtc attacacagg cctgtccaaa ggtatcattt gagccaattc ccatacatta
     1141 ttgtgccccg gctggttttg cgattctaaa gtgtaataat aaaacgttca atggaaaagg
     1201 accatgtaca aatgtcagca cagtacaatg tacacatgga attaagccag tagtgtcaac
     1261 tcaactgctg ttaaatggca gtctagcaga agaagaggta gtaattagat ctgacaattt
     1321 cacggacaat gctaaaacca taatagtaca gctgaatgaa tctgtagcaa ttaattgtac
     1381 aagacccaac aacaatacaa gaaaaggtat ccatatagga ccagggagag cagtttatac
     1441 aacaggaaga ataataggag atataagaaa agcacattgt aacgttagta gaacacaatg
     1501 gaataacact ttagaacaga tagttaaaaa attaagagaa cagtttggga ataataaaac
     1561 aatagtcttt aatcaatcct caggagggga cccagaaatt gtaatgcaca gttttaattg
     1621 tagaggggaa tttttctact gtaatacagc acaactgttt aatagtactt ggaatccaaa
     1681 taacactgaa ggaaataaca ctgacccaat catactccca tgtagaataa aacaaattat
     1741 aaacatgtgg caggaagtag gaaaagcaat gtatgcccct cccattggag gacaaattag
     1801 ttgttcatca aacattacag ggctgctatt aacaagagat ggtggtataa atggaactaa
     1861 tgacaccgag gtcttcagac ctggaggagg agatatgagg gacaattgga gaagtgaatt
     1921 atataaatat aaagtagtaa aaattgaacc attaggaata gcacccacca aggcaaagag
     1981 aagagtggtg cagagagaaa aaagagcagt gggaataata ggagctatgt tccttgggtt
     2041 cttgggagca gcaggaagca ctatgggcgc acggtcaatg acgctgacgg tacaggccag
     2101 aaaattattg tctggtatag tgcaacagca gaacaatttg ctgagggcta ttgaggcgca
     2161 acaacatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gagtcctggc
     2221 tgtggaaaga tacctaaggg atcaacagct cctagggatt tggggttgct ctggaaaact
     2281 catctgcacc actgctgtgc cttggaatac tagttggagt aataaatcta tggaagacat
     2341 ttgggataac atgacctgga tgcagtggga aaaagaaatt gacaattaca caaacacaat
     2401 atacacctta cttgaagaat cgcagaacca acaagaaaag aatgaacaag aattattgga
     2461 attggataag tgggcaagtt tgtggaattg gtttaacata acaaactggc tgtggtatat
     2521 aaaaatattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
     2581 ttctgtagtg aatagagtta ggcagggata ctcaccatta tcatttcaga cccgcctccc
     2641 aaccccgagg ggacccgaca ggcccgacgg aatcgaagaa gaaggtggag agagagacag
     2701 agacagatcc gttcgattag tggatggatt cttagcactt atctgggaag atctgcggag
     2761 cctgtgcctc ttcagctacc gccgcttgag agacttactc ttgattgcag tgaggactgt
     2821 ggaaattctg ggacacaggg ggtgggaagt cctcaaatat gggtggagtc tcctgcagta
     2881 ttggattcag gaactaagga atagtgctgt tagcttgctc aacgccacag ctatagcagt
     2941 agctgagggg acagataggg ttatagaagt agtacaaaga gcttatagag ctattctcca
     3001 catacctaga agaattagac agggcttaga aaggcttttg ctataagatg ggtggcaagt
     3061 ggtcaaaacg tagtatgggt ggatggtctg ctataaggga aagaatgaga cgagctgagc
     3121 cacgagctga gccagcagca gatggggtgg gagcagtatc tggagacctg gaaaaacatg
     3181 gagcaatcac aagtagcaat acagcagcta ctaatgctga ttgtgcctgg ctagaagcac
     3241 aagaggagga agaggtgggt tttccagtca gacctcaggt acctttaaga ccaatgactt
     3301 acaaggcagc tgtagatatt agccactttt taaaagaaaa ggggggactg gaagggctaa
     3361 tttggtccca aagaaggcaa gagatccttg atctgtggat ctaccacaca caaggctact
     3421 tccctgattg gcagaactac acaccagggc cagggatcag atatccactg acctttggat
     3481 ggtgcttcaa gctagtacca cttgagccag agcaggtaga agaggccaat gaaggagaga
     3541 acaacagctt gttacaccct ataagccagc atgggatgga tgacccggag aaagaagtgt
     3601 tagtgtggaa atttgacagc aagctagcat ttcatcacat ggcccgagag ctgcatccgg
     3661 agtactacaa agactgctga catcgagctt tctacagggg actttccgct ggggactttc
     3721 cagggaggcg tggcctgggc gggactgggg agtggcgtgc cctcagatgc tgcatataag
     3781 cagctgcttt ttgcctgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
     3841 tctggctagc tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     3901 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     3961 tcagtatgga aaatctctag cag
//