From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human immunodeficiency virus type 1, isolate JRCSF; complete
Date: 29 Sep 92 10:05:27 GMT
Distribution: bionet
Checksum: 41872    20

LOCUS       HIVJRCSF     9540 bp ss-RNA             VRL       29-SEP-1992
DEFINITION  Human immunodeficiency virus type 1, isolate JRCSF; complete
            genome.
ACCESSION   M38429
KEYWORDS    long terminal repeat (LTR).
SOURCE      HIV-1 proviral DNA from extracellular virus taken from cerebral
            spinal fluid (1986). Infectious clone.
  ORGANISM  Human immunodeficiency virus type 1
            Viridae; ss-RNA enveloped viruses; Positive strand RNA virus;
            Retroviridae; Lentivirinae.
REFERENCE   1  (bases 1 to 9540)
  AUTHORS   Koyanagi,S. and Chen,I.S.
  JOURNAL   Unpublished (1988) UCLA School of Medicine, Los Angeles.
  STANDARD  full staff_entry
COMMENT
            Kindly provided in computer-readable form by Irvin Chen, UCLA
            School of Medicine, Los Angeles. JRCSF and JRFL (see <HIVJRFL> were
            isolated from cerebral spinal fluid and brain tissue of the patient
            JR, who died with Kaposi's sarcoma and severe AIDS encepha- lopathy
            (Science 236, 819-822, 1987). Both clones are infectious, but JRFL
            productively infects macrophages while JRCSF does not.  (Peripheral
            blood was not available from the patient).
            The JRCSF and JRFL env nucleotide sequences differ by at least 3%;
            further characterization of them is forthcoming (Peng,S. et al.,
            Nature 1990, in press). Both manifest insertions in nef previously
            reported for HIVBRVA.
FEATURES             Location/Qualifiers
     CDS             <2085..5108
                     /product="pol polyprotein"
                     /gene="pol"
                     /codon_start=1
                     /translation="FFREDLAFLQGKAREFPSEQTRANSPTRRELQVWGRDSNSLSEA
                     GAEAGADRQGIVSFNFPQITLWQRPLVTIKIGGQLKEALLDTGADDTVLEDMDLPGRW
                     KPKMIGGIGGFIKVRQYDQIPIDICGHKAVGTVLVGPTPVNIIGRNLLTQIGCTLNFP
                     ISPIETVPVKLKPGMDGPKVKQWPLTEEKIKALVEICTEMEKEGKISKIGPENPYNTP
                     VFAIKKKDSTKWRKLVDFRELNRRTQDFWEVQLGIPHPAGLKKKKSVTVLDVGDAYFS
                     VPLDKDFRKYTAFTIPSINNETPGIRYQYNVLPQGWKGSPAIFQSSMTKILEPFRKQN
                     PDIIIYQYMDDLYVGSDLEIGQHRTKIEELRQHLLKWGFTTPDKKHQKEPPFLWMGYE
                     LHPDKWTVQPIVLPEKDSWTVNDIQKLVGKLNWASQIYAGIKVKQLCKLLRGTKALTE
                     VIPLTKEAELELAENREILKEPVHGVYYDPSKDLIVEIQKQGQGQWTYQIFQEPFKNL
                     KTGKYARTRGAHTNDVKQLTEAVQKIANESIVIWGKIPKFKLPIQKETWETWWTEYWQ
                     ATWIPEWEFVNTPPLVKLWYQLEKEPIVGAETFYVDGAANRETKLGKAGYVTSRGRQK
                     VVSLTDTTNQKTELQAIHLALQDSGLEVNIVTDSQYALGIIQAQPDKSESELVSQIIE
                     QLIKKEKVYLAWVPAHKGIGGNEQVDKLVSAGIRKVLFLDGIDKAQEDHEKYHSNWRA
                     MASDFNLPPIVAKEIVASCDKCQLKGEAMHGQVDCSPGIWQLDCTHLEGKIILVAVHV
                     ASGYIEAEVIPAETGQETAYFLLKLAGRWPVTTIHTDNGSNFTSTTVKAACWWAGIKQ
                     EFGIPYNPQSQGVVESMNKELKKIIGQVRDQAEHLKTAVQMAVFIHNFKRKGGIGGYS
                     AGERIIDIIATDIQTKELQKQITKIQNFRVYYRDNRDPIWKGPAKLLWKGEGAVVIQD
                     NSDIKVVPRRKVKIIRDYGKQMAGDDCVASRQDED"
     LTR             1..635
                     /partial
     protein_bind    378..387
                     /bound_moiety="Sp1"
     protein_bind    389..398
                     /bound_moiety="Sp1"
     protein_bind    400..409
                     /bound_moiety="Sp1"
     CDS             790..2304
                     /product="gag polyprotein"
                     /gene="gag"
                     /codon_start=1
                     /translation="MGARASVLSGGELDRWEKIRLRPGGKKKYRLKHIVWASRELERF
                     AVNPGLLESSEGCRQILGQLQPSLKTGSEELTSLYNTVATLYCVHQRIEIKDTKEALE
                     KIEEEQTKSMKKAQQAAADTGNSSQVSQNYPIVQNLQGQMVHQAISPRTLNAWVKVIE
                     EKAFSPEVIPMFSALSEGATPQDLNTMLNTVGGHQAAMQMLKETINEEAAEWDRLHPV
                     HAGPIAPGQMREPRGSDIAGTTSTLQEQIGWMTNNPPIPVGEIYKRWIILGLNKIVRM
                     YSPVSILDIRQGPKEPFRDYVDRFYKTLRAEQATQEVKNWMTETLLVQNANPDCKTIL
                     KALGPAATLEEMMTACQGVGGPGHKARVLAEAMSQVTNPATIMMQRGNFRNQRKNVKC
                     FNCGKEGHIARNCRAPRKKGCWKCGKEGHQMKECTERQANFLGKIWPSYKGRPGNFLQ
                     SRPEPTAPPEESFRFGEETATPSQKQEQKQEPIDKELYPLTSLRSLFGNDPSSQ"
     CDS             5053..5631
                     /product="vif protein"
                     /gene="vif"
                     /codon_start=1
                     /translation="MENRWQVMIVWQVDRMRIRTWNSLVKHHMYISGKAKGWIYKHHY
                     ESTNPRVSSEVQIPLGDARLVITTYWGLHTGERDWHLGQGVSMEWRTRRYSTQVDPDL
                     ADQLIHLYYFDCFSESAIRNAILGHIVSPRCEYQAGHSKVGSLQYLALTALIKPKKIK
                     PPLPSVKKLTEDRWNKPQKTKGHRGSHTMNGH"
     CDS             5571..5861
                     /gene="vpR"
                     /codon_start=1
                     /translation="MEQAPEDQGPQREPYNEWTLELLEELKNEAVRHFPRIWLHSLGQ
                     YIYETYGDTWAGVEAIIRILQQLLFIHFRIGCRHSRIGITRQRRARNGASRS"
     exon            5842..6056
                     /number=2
                     /gene="tat"
     exon            5981..6056
                     /number=2
                     /gene="rev"
     CDS             6073..6318
                     /gene="vpU"
                     /codon_start=1
                     /translation="MQPLQILAIVALVVAGIIAIIVWSIVLIEYRKILRQRKIDRLID
                     KIRERAEDSGNESEGDQEELSALVERGHLAPWDINDL"
     CDS             6236..8782
                     /product="envelope polyprotein"
                     /gene="env"
                     /codon_start=1
                     /translation="MRVKGIRKNYQHLWKGGILLLGTLMICSAVEKLWVTVYYGVPVW
                     KETTTTLFCASDAKAYDTEVHNVWATHACVPTDPNPQEVVLENVTEDFNMWKNNMVEQ
                     MQEDVINLWDQSLKPCVKLTPLCVTLNCKDVNATNTTSSSEGMMERGEIKNCSFNITK
                     SIRDKVQKEYALFYKLDVVPIDNKNNTKYRLISCNTSVITQACPKVSFEPIPIHYCAP
                     AGFAILKCNNKTFNGKGQCKNVSTVQCTHGIRPVVSTQLLLNGSLAEEKVVIRSDNFT
                     DNAKTIIVQLNESVKINCTRPSNNTRKSIHIGPGRAFYTTGEIIGDIRQAHCNISRAQ
                     WNNTLKQIVEKLREQFNNKTIVFTHSSGGDPEIVMHSFNCGGEFFYCNSTQLFNSTWN
                     DTEKSSGTEGNDTIILPCRIKQIINMWQEVGKAMYAPPIKGQIRCSSNITGLLLTRDG
                     GKNESEIEIFRPGGGDMRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIG
                     ALFLGFLGAAGSTMGARSMTLTVQARQLLSGIVQQQNNLLRAIEAQQHMLQLTVWGIK
                     QLQARVLAVERYLKDQQLMGIWGCSGKLICTTAVPWNTSWSNKSLDSIWNNMTWMEWE
                     KEIENYTNTIYTLIEESQIQQEKNEQELLELDKWASLWNWFGITKWLWYIKIFIMIVG
                     GLIGLRIVFSVLSIVNRVRQGYSPLSFQTLLPATRGPDRPEGIEEEGGERDRDRSGQL
                     VNGFLALIWVDLRSLFLFSYHRLRDLLLTVTRIVELLGRRGWEILKYWWNLLQYWSQE
                     LKNSAVSLLNATAIAVAEGTDRIIEVVQRVYRAILHIPTRIRQGLERALL"
     exon            8366..8456
                     /number=3
                     /gene="tat"
     exon            8366..8640
                     /number=3
                     /gene="rev"
     CDS             8784..9434
                     /gene="nef"
                     /codon_start=1
                     /translation="MGGKWSKHSVPGWSTVRERMRRAEPATDRVRQTEPAAVGVGAVS
                     RDLEKHGAITSSNTAATNADCAWLEAYEDEEVGFPVRPQVPLRPMTYKAAIDLSHFLK
                     EKGGLEGLIYSQKRQDILDLWIYHTQGYFPDWQNYTAGPGVRFPLTFGWCFKLVPVDP
                     EKVEEANEGENNCLLHPMSQHGMDDPEKEVLVWKFDSKLALHHVARELHPEYYKDC"
     LTR             9103..9540
                     /partial
BASE COUNT     3425 a   1691 c   2308 g   2116 t
ORIGIN      5' terminus of 5'LTR.
        1 ctggaagggc taatttactc acagaaaaga caagatatcc ttgatctgtg gatctaccac
       61 acacaaggct acttccctga ttggcagaac tacacagcag gaccaggggt cagatttcca
      121 ctgacctttg gatggtgctt caagctagta ccagttgatc cagagaaggt agaagaggcc
      181 aatgaaggag agaacaactg cttgttacac cctatgagcc agcatggaat ggacgaccca
      241 gagaaggaag tgttagtgtg gaagtttgac agcaagctag cattgcatca cgtggcccga
      301 gagctgcatc cggagtacta caaggactgc tgacaccgag ctttctacaa gggactttcc
      361 gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga
      421 tgctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag accagatctg
      481 agcctgggag ctctctggct agctagggaa cccactgctt aagcctcaat aaagcttgcc
      541 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
      601 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggaccggaaa
      661 gcgaaagaga aaccagagga gatctctcga cgcaggactc ggcttgctga agcgcgcaca
      721 gcaagaggcg aggggcggcg actggtgagt acgccgaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattgga taggtgggaa
      841 aaaattcggt taaggccagg aggaaagaaa aaatatagat taaaacatat agtatgggca
      901 agcagggagc tagaacgttt cgcagtcaat cctggcctgt tagaatcatc agaaggctgt
      961 agacaaatac tgggacaact acaaccatcc cttaagacag gatcagaaga acttacatca
     1021 ttatataata cagtagcaac cctctattgt gtacatcaaa ggatagagat aaaagacacc
     1081 aaggaagctt tagaaaagat agaggaagag caaaccaaaa gtatgaaaaa ggcacagcaa
     1141 gcagcagctg acacaggaaa cagcagccag gtcagccaaa attaccctat agtgcagaac
     1201 ctgcaggggc aaatggtaca tcaggccata tcacctagaa ctttaaatgc atgggtaaaa
     1261 gtaatagaag agaaggcttt cagccccgaa gtaataccca tgttttcagc attatcagaa
     1321 ggagccaccc cacaagattt aaacaccatg ctaaacacag tggggggaca tcaagcagct
     1381 atgcaaatgc taaaagaaac catcaatgag gaagctgcag aatgggatag attgcatcca
     1441 gtgcatgcag ggcctattgc accaggccag atgagagaac caaggggaag tgacatagca
     1501 gggactacta gtacccttca ggaacaaata ggatggatga caaataatcc acctatccca
     1561 gtaggagaaa tctataaaag atggataatc ctggggttaa ataaaatagt aaggatgtat
     1621 agccctgtca gcattctgga cataagacaa ggaccaaagg aaccctttag agactatgta
     1681 gaccggttct ataaaaccct aagagccgag caagctacac aggaggtaaa aaattggatg
     1741 acagaaacct tgttggtcca aaatgcgaac ccagattgta aaactatttt aaaagcattg
     1801 ggaccagcag ctacactaga agaaatgatg acagcatgtc agggagtggg aggacccggc
     1861 cataaagcaa gagttttggc tgaagcaatg agccaagtaa caaatccagc taccataatg
     1921 atgcagagag gcaactttag gaaccaaaga aagaatgtta agtgtttcaa ttgtggcaaa
     1981 gaagggcaca tagccagaaa ttgcagggcc cctaggaaaa agggctgttg gaaatgtgga
     2041 aaggaaggac accaaatgaa agagtgtact gagagacagg ctaatttttt agggaagatc
     2101 tggccttcct acaagggaag gccagggaat ttccttcaga gcagaccaga gccaacagcc
     2161 ccaccagaag agagcttcag gtttggggaa gagacagcaa ctccctctca gaagcaggag
     2221 cagaagcagg agccgataga caaggaattg tatcctttaa cttccctcag atcactcttt
     2281 ggcaacgacc cctcgtcaca ataaagatag gggggcaact aaaggaagct ctattagata
     2341 caggagcaga tgatacagta ttagaagaca tggatttgcc aggaagatgg aaaccaaaaa
     2401 tgataggggg aattggaggt tttatcaaag taagacagta tgatcagata cccatagata
     2461 tctgtggaca taaagctgta ggtacagtat tagtaggacc tacacctgtc aacataattg
     2521 gaagaaatct gttgactcag attggttgca ctttaaattt tcccattagt cctattgaaa
     2581 ctgtaccagt aaaattaaag ccaggaatgg atggcccaaa agtcaaacaa tggccattga
     2641 cagaagaaaa aataaaagca ttagtagaaa tttgtacaga aatggaaaag gaaggaaaga
     2701 tttcaaaaat tgggcctgaa aatccataca atactccagt atttgccata aagaaaaaag
     2761 acagtactaa atggagaaaa ttagtagatt tcagagaact taataggaga actcaagact
     2821 tctgggaagt tcaattagga ataccacatc ccgcagggtt aaaaaagaaa aaatcagtaa
     2881 cagtactgga tgtgggtgat gcatattttt cagttccctt agataaagac ttcaggaagt
     2941 atactgcatt taccatacct agtataaaca atgagacacc agggattaga tatcagtaca
     3001 atgtgcttcc acagggatgg aaaggatcac cagcaatatt ccaaagtagc atgacaaaaa
     3061 tcttagagcc ttttagaaaa caaaatccag acataattat ctatcaatac atggatgatt
     3121 tgtatgtagg atctgactta gaaatagggc agcatagaac aaaaatagag gaactgagac
     3181 aacatctgtt gaagtgggga tttaccacac cagacaaaaa acatcagaaa gaacctccat
     3241 tcctttggat gggttatgaa ctccatcctg ataaatggac agtacagcct atagtgctgc
     3301 cagaaaaaga cagctggact gtcaatgaca tacagaagtt agtgggaaaa ttgaattggg
     3361 caagtcaaat ttatgcaggg attaaagtaa agcaattatg taaactcctt aggggaacca
     3421 aagcacttac agaagtaata ccactaacaa aagaagcaga gctagaactg gcagaaaaca
     3481 gggagattct aaaagaacca gtacatggag tgtattatga cccatcaaaa gacttaatag
     3541 tagaaataca gaagcagggg caaggccaat ggacatatca aatttttcaa gagccattta
     3601 aaaatctgaa aacaggaaaa tatgcaagaa cgaggggtgc ccacactaat gatgtaaaac
     3661 aattaacaga ggcagtgcaa aaaatagcca atgaaagcat agtaatatgg ggaaagattc
     3721 ctaaatttaa attacccata caaaaagaaa catgggaaac atggtggaca gagtattggc
     3781 aagccacctg gattcctgag tgggagtttg tcaatacccc tcccttagtg aaattatggt
     3841 accagttaga aaaagaaccc atagtaggag cagaaacttt ctatgtagat ggggcagcta
     3901 acagggagac taaattagga aaagcaggat atgttactag cagaggaaga caaaaagttg
     3961 tctccctaac agacacaaca aatcagaaaa ctgagttaca agcaattcac ctagctttgc
     4021 aggattcagg attagaagta aacatagtaa cagactcaca atatgcatta ggaatcattc
     4081 aagcacaacc agataaaagt gaatcagagt tagtcagtca aataatagaa cagctaataa
     4141 aaaaggaaaa agtctacctg gcatgggtac cagcacacaa aggaattgga ggaaatgaac
     4201 aggtagataa attagtcagt gctggaatca ggaaagtgct atttttagat ggaatagata
     4261 aggcccaaga agatcatgaa aaatatcaca gtaattggag agcaatggct agtgatttta
     4321 acctgccacc tatagtagca aaagaaatag tagccagctg tgataaatgt cagctaaaag
     4381 gagaagccat gcatggacaa gtagactgta gtccaggaat atggcaacta gattgtacac
     4441 atttagaagg aaaaattatc ctggtagcag ttcatgtagc cagtggatat atagaagcag
     4501 aagttattcc agcagaaaca gggcaggaaa cagcatactt tctcttaaaa ttagcaggca
     4561 gatggccagt aacaacaata catacagaca atggcagcaa tttcaccagt actacagtta
     4621 aggccgcctg ttggtgggct gggatcaagc aggaatttgg cattccctac aatccccaaa
     4681 gtcaaggagt agtagaatct atgaataaag aattaaagaa aattatagga caggtaagag
     4741 atcaggctga acatcttaag acagcagtac aaatggcagt attcatccac aattttaaaa
     4801 gaaaaggggg gattgggggg tacagtgcag gggaaagaat aatagacata atagcaacag
     4861 acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt
     4921 acagggacaa cagagatcca atttggaaag gaccagcaaa gcttctctgg aaaggtgaag
     4981 gggcagtagt aatacaagat aatagtgaca taaaagtagt gccaagaaga aaagtaaaaa
     5041 tcattaggga ttatggaaaa cagatggcag gtgatgattg tgtggcaagt agacaggatg
     5101 aggattagaa catggaacag tttagtaaaa caccatatgt atatttcagg gaaagctaag
     5161 ggatggattt ataaacatca ctatgaaagc actaatccaa gagtaagttc agaagtacaa
     5221 atcccactag gggatgctag attggtaata acaacatatt ggggtctgca tacaggagaa
     5281 agagactggc atttgggtca gggagtctcc atggaatgga ggacaaggag atatagcaca
     5341 caagtagacc ctgacctagc agaccaacta attcatctgt attactttga ttgtttttca
     5401 gaatctgcta taaggaatgc catattagga catatagtta gtcctagatg tgaatatcaa
     5461 gcaggacata gcaaggtagg atctctacag tacttggcac taacagcatt aataaaacca
     5521 aaaaagataa agccaccttt gcctagtgtt aagaaactaa cagaggatag atggaacaag
     5581 ccccagaaga ccaagggcca cagagggagc catacaatga atggacacta gagcttttag
     5641 aggaacttaa gaatgaagct gttagacatt ttcctaggat ctggctccat agcttagggc
     5701 aatatatcta tgaaacttat ggggatactt gggcaggagt ggaagccata ataagaatac
     5761 tgcaacagct gctgtttatt catttcagaa ttgggtgtcg acatagcaga ataggcatta
     5821 ctcgacagag gagagcaaga aatggagcca gtagatccta gcctagagcc ctggaagcat
     5881 ccaggaagtc agcctaagac tgcttgtacc aattgctatt gtaaaaagtg ttgccttcat
     5941 tgccaagttt gtttcacaac aaaaggctta ggcatctcct atggcaggaa gaagcggaga
     6001 cagcgacgaa gacctcctca agacagtcag actcatcaag tttctctacc aaagcagtaa
     6061 gtagtgcatg taatgcaacc tttacaaata ttagcaatag tagcattagt agtagcagga
     6121 ataatagcaa taattgtgtg gtccatagta ctcatagaat ataggaaaat attaagacaa
     6181 agaaaaatag ataggttaat tgataaaata agagagagag cagaagacag tggcaatgag
     6241 agtgaagggg atcaggaaga attatcagca cttgtggaaa gggggcatct tgctccttgg
     6301 gacattaatg atctgtagtg ctgtagaaaa gttgtgggtc acagtctatt atggggtacc
     6361 tgtgtggaaa gaaacaacca ccactctatt ttgtgcatca gatgctaaag catatgatac
     6421 agaggtacat aatgtttggg ccacacatgc ctgtgtaccc acagacccca acccacaaga
     6481 agtagtattg gaaaatgtaa cagaagattt taacatgtgg aaaaataaca tggtagaaca
     6541 gatgcaggag gatgtaatca atttatggga tcaaagctta aagccatgtg taaaattaac
     6601 cccactctgt gttactttaa attgcaaaga tgtgaatgct actaatacca ctagtagtag
     6661 tgagggaatg atggagagag gagaaataaa aaactgctct ttcaatatca ccaaaagcat
     6721 aagagataag gtgcagaaag aatatgctct tttttataaa ctggatgtag taccaataga
     6781 taataagaat aataccaaat ataggttaat aagttgtaac acctcagtca ttacacaagc
     6841 ctgtccaaag gtatcctttg aaccaattcc catacattat tgtgccccgg ctggttttgc
     6901 gattctaaag tgtaataata agacattcaa tggaaaagga caatgtaaaa atgtcagcac
     6961 agtacaatgt acacatggaa ttaggccagt agtatcaact caactgctgc taaatggcag
     7021 tctagcagaa gaaaaggttg taattagatc tgacaatttt acggacaatg ctaaaaccat
     7081 aatagtacag ctgaatgaat ctgtaaaaat taattgtaca aggcccagca acaatacaag
     7141 aaaaagtata catataggac cagggagagc attttataca acaggagaaa taataggaga
     7201 tataagacaa gcacattgta acattagtag agcacaatgg aataacactt taaaacagat
     7261 agttgaaaaa ttaagagaac aatttaataa taaaacaata gtctttactc actcctcagg
     7321 aggggatcca gaaattgtaa tgcacagttt taattgtgga ggggaatttt tctactgtaa
     7381 ttcaacacaa ctgtttaata gtacttggaa tgatactgaa aagtcaagtg gcactgaagg
     7441 aaatgacacc atcatactcc catgcagaat aaaacaaatt ataaacatgt ggcaggaagt
     7501 gggaaaagca atgtatgctc ctcccattaa aggacaaatt agatgttcat caaatattac
     7561 agggctgcta ttaacaagag atggtggtaa aaatgagagt gagatcgaga tcttcagacc
     7621 tggaggagga gacatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa
     7681 aattgaacca ttaggagtag cacccaccaa ggcaaagaga agagtggtgc aaagagaaaa
     7741 aagagcagtg ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat
     7801 gggcgcacgg tcaatgacac tgacggtaca ggccagacaa ttattgtctg gtatagtgca
     7861 acagcaaaac aatttgctga gggctattga ggcgcaacag catatgttgc aactcacagt
     7921 ctggggcatc aagcagctcc aggcaagagt cctggctgtg gaaagatacc taaaggatca
     7981 acagctcatg gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg
     8041 gaatactagt tggagtaata aatctctgga tagtatttgg aataacatga cctggatgga
     8101 gtgggaaaaa gaaattgaga attacacaaa cacaatatac accctaattg aagaatcgca
     8161 gatccaacaa gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg
     8221 gaattggttt ggcataacaa aatggctgtg gtatataaaa atattcataa tgatagtagg
     8281 aggcttgata ggtttaagaa tagttttttc tgtactttct atagtgaata gagttaggca
     8341 gggatactca cccttatcgt ttcagaccct cctcccagca acgaggggac ccgacaggcc
     8401 cgaaggaatc gaagaagaag gtggagagag agacagagac agatccggac aattagtgaa
     8461 cggattctta gcacttatct gggtcgacct gcggagcctg ttcctcttca gctaccaccg
     8521 cttgagagac ttactcttga ctgtaacgag gattgtggaa cttctgggac gcagggggtg
     8581 ggaaatcctg aaatactggt ggaatctcct acagtattgg agtcaggaac taaagaatag
     8641 tgctgttagc ttgcttaatg ccacagctat agcagtagct gaggggacag ataggattat
     8701 agaagtagta caaagagttt atagggctat tctccacata cctacaagaa taagacaggg
     8761 cttggaaagg gctttgctat aagatgggtg gcaagtggtc aaaacatagt gtgcctggat
     8821 ggtctactgt aagggaaaga atgagacgag ctgagccagc aacagatagg gtgagacaaa
     8881 ctgagccagc agcagtaggg gtgggagcag tatctcgaga cctggaaaaa catggagcaa
     8941 tcacaagtag caatacagca gctaccaatg ctgattgtgc ctggctagaa gcatatgagg
     9001 atgaggaagt gggttttcca gtcagacctc aggtaccttt aagaccaatg acttacaagg
     9061 cagctataga tcttagccac tttttaaaag aaaagggggg actggaaggg ctaatttact
     9121 cacagaaaag acaagatatc cttgatctgt ggatctacca cacacaaggc tacttccctg
     9181 attggcagaa ctacacagca ggaccagggg tcagatttcc actgaccttt ggatggtgct
     9241 tcaagctagt accagttgat ccagagaagg tagaagaggc caatgaagga gagaacaact
     9301 gcttgttaca ccctatgagc cagcatggaa tggacgaccc agagaaggaa gtgttagtgt
     9361 ggaagtttga cagcaagcta gcattgcatc acgtggcccg agagctgcat ccggagtact
     9421 acaaggactg ctgacaccga gctttctaca agggactttc cgctggggac tttccaggga
     9481 ggcgtggctg ggcgggactg gggagtggcg agccctcaga tgctgcatat aagcagctgc
//