From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: R.norvegicus mRNA for MAP kinase kinase
Date: 31 Dec 92 01:47:54 GMT
Distribution: bionet
Checksum: 40526 6

LOCUS       RATMAPKL     2136 bp ds-DNA             ROD       18-DEC-1992
DEFINITION  R.norvegicus mRNA for MAP kinase kinase
ACCESSION   Z16415
KEYWORDS    MAP kinase kinase; protein kinase.
SOURCE      Rattus norvegicus RNA.
  ORGANISM  Rattus norvegicus
            Eukaryota; Animalia; Chorqata; Vertebrata; Mammalia; Theria;
            Eutheria; Rodentia; Myomorpha; Muridae; Murinae.
REFERENCE   1  (sites)
  AUTHORS   Wu,J.
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
REFERENCE   2  (sites)
  AUTHORS   Wu,J., Michel,H., Rossomando,A., Haystead,T.A., Shabanowitz,J.,
            Hunt,D.F. and Sturgill,T.W.
  TITLE     Renaturation and partial peptide sequencing of mitogen-activated
            protein kinase (MAP kinase) activator from rabbit skeletal muscle
  JOURNAL   Biochem. J. 285, 701-705 (1992)
  STANDARD  full automatic
REFERENCE   3  (bases 1 to 2136; 1 to 2136)
  AUTHORS   Wu,J., Harrison,J.K., Vincent,L.A., Haystead,C., Haystead,T.A.,
            Michel,H., Hunt,D.F., Lynch,K.R. and Sturgill,T.W.
  TITLE     Molecular structure of the protein-tyrosine/threonine kinase
            activating p42 mitogen-activated protein (MAP) kinase: MAP kinase
            kinase
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. (1992) In press
  STANDARD  full automatic
COMMENT     *source: strain=Sprague-Dawley; *source: tissue=Kidney; *source:
            clone_library=Lamda gt10; *source: clone=pK28;
               From EMBL    entry RNMAPKK;  dated 07-OCT-1992.
FEATURES             Location/Qualifiers
     mRNA            19..2128
     mat_peptide     34..1209
                     /function="Protein tyrosine/threonine kinasespecific for
                     MAP kinase"
                     /standard_name="MAP kinase kinase"
                     /product="MAP kinasekinase"
                     /codon_start=1
     misc_signal     133..177
                     /function="Encode a bipartite nuclear targetingsequence"
     polyA_signal    2084..2089
     polyA_site      2108
                     /evidence="EXPERIMENTAL"
     CDS             31..1212
                     /function="Dual specificity proteintyrosine/threonine
                     kinase"
                     /standard_name="MAP kinase kinase"
                     /product="proteinkinase"
                     /codon_start=1
                     /translation="MPKKKPTPIQLNPAPDGSAVNGTSSAETNLEALQKKLEELELDE
                     QQRKRLEAFLTQKQKVGELKDDDFEKISELGAGNGGVVFKVSHKPSGLVMARKLIHLE
                     IKPAIRNQIIRELQVLHECNSPYIVGFYGAFYSDGEISICMEHMDGGSLDQVLKKAGR
                     IPEQILGKVSIAVIKGLTYLREKHKIMHRDVKPSNILVNSRGEIKLCDFGVSGQLIDS
                     MANSFVGTRSYMSPERLQGTHYSVQSDIWSMGLSLVEMAVGRYPIPPPDAKELELLFG
                     CQVEGDAAETPPRPRTPGRPLSSYGMDSRPPMAIFELLDYIVNEPPPKLPSGVFSLEF
                     QDFVNKCLIKNPAERADLKQLMVHAFIKRSDAEEVDFAGWLCSTIGLNQPSTPTHAAS
                     I"
BASE COUNT      551 a    487 c    542 g    556 t
ORIGIN      
        1 gaattccgcg gaattccgcc cggctgcaag atgcccaaga agaagccgac gcccatccag
       61 ctgaacccgg cccccgatgg ctccgcggtt aacgggacca gctcggccga gaccaacctg
      121 gaggccttgc agaagaagct ggaggagctg gagctggacg agcagcagcg gaagcgcctt
      181 gaggcctttc tgacgcagaa gcagaaggtg ggagagttga aggatgatga ctttgagaag
      241 atcagtgaac tgggggctgg caatggtgga gtggtgttca aggtctccca caagccatct
      301 ggcctggtta tggctaggaa gctaattcac ctggagatca aacccgcaat ccggaaccag
      361 atcatccggg agctgcaggt gctgcatgag tgcaactccc cgtacatagt gggcttctac
      421 ggggccttct acagtgacgg cgagatcagc atctgcatgg agcacatgga tggtgggtcc
      481 ttggatcaag tgctgaagaa agctggaaga attcctgagc aaattttagg aaaagtcagc
      541 atcgctgtga taaaaggcct gacatatcta cgagagaagc acaagattat gcacagagat
      601 gtcaagcctt ccaacattct agtgaactca cgtggggaga tcaaactctg cgattttggg
      661 gtcagcgggc agctaattga ctccatggcc aactccttcg tgggaacaag gtcctacatg
      721 tcgcctgaga gactccaggg gactcactac tctgtgcagt cggacatctg gagcatgggg
      781 ctctctctgg tggagatggc agttggaaga taccccattc ctcctcctga tgccaaggag
      841 ctggagctgc tgtttggatg ccaggtggaa ggagacgcgg ccgaaacgcc acccaggcca
      901 aggacccctg ggaggcccct cagctcatat ggaatggata gccgacctcc catggcaatt
      961 tttgagttgt tggattacat cgtcaatgag cctcctccaa aactgcccag tggagtattc
     1021 agtctggaat ttcaggattt tgtgaataag tgcttaataa agaaccctgc agagagagca
     1081 gatctgaagc agctcatggt acatgctttc atcaagagat ctgatgccga ggaggtagac
     1141 ttcgcaggct ggctctgctc caccattggg cttaaccagc ccagcacacc aacccacgct
     1201 gccagcatct gagcctttgg gaagcagcag agaggaatcc tctgcccagt ggcatgccat
     1261 gttgctttca ggcctctccc atgcttgtct atgttcagat gtgcatctca tctgtgacaa
     1321 aggatgaaga acacagcatg tgccaaatcg tacttgtgtc atttttaata ttgtctttat
     1381 cgctatggtt actcccctaa gtggattggc tttgtgcttg gggctatttg tctgttcatc
     1441 aaatacatgc caggttgaac tacagtgaaa ccctggtgac ctgggtggtc ttcttactga
     1501 tgtttgcgct gctgttcatc gtgactcact agctggctgc ctgtattgtc aggattctcg
     1561 gacccttggt acttcactct tgctggtgac ctctcagtct gaggagaggg ggccttctga
     1621 gacccttcac aggcagtgca tgcatgaaaa gcatgctttg ctgctactga aatgagcacc
     1681 agaacgtgta catcatggta ttttattttt gcttttggta tagaactcag cagttcccat
     1741 ttaaaaaaaa aatctaacca gagcccatca ctgccatgat agctggggct tcagtctgtc
     1801 tactgtggtg attttagact tctggttgta tttctatatt tatttttaaa tatactgtgt
     1861 gggatactta gtggtatatg tctctgagtt tggattagtg tttctaaatt ggtagttatt
     1921 ttgaatgtca caaatggatt aaggaatcaa cgtatcaaga gttctatctt tcttccagtc
     1981 taagtaccaa tgctattgta aacgtgtata gtgcctacaa attgtatgaa aaccctttta
     2041 accactttac tcaagatgtt tatcaaatct aatctcttat tctaataaaa atactatcaa
     2101 gttaaagtaa aaaaaaaaaa aaaaaaaacg gaattc
//