From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Leishmania donovani multidrug-resistance P-glycoprotein (ldmdr1)
Date: 15 Sep 92 10:04:45 GMT
Distribution: bionet
Checksum: 18206     9

LOCUS       LEILDMDR1A   4690 bp ds-DNA             INV       15-SEP-1992
DEFINITION  Leishmania donovani multidrug-resistance P-glycoprotein (ldmdr1)
            gene, complete cds.
ACCESSION   L01572
KEYWORDS    P-glycoprotein; multidrug-resistance.
SOURCE      Leishmania donovani (individual_isolate DI700, strain 1S Sudanese)
            DNA.
  ORGANISM  Leishmania donovani
            Eukaryota; Animalia; Protozoa; Sarcomastigophora; Mastigophora;
            Kinetoplastida; Trypanosomatina; Trypanosomatidae.
REFERENCE   1  (bases 1 to 4690)
  AUTHORS   Ullman,B., Wirth,D.F., Allen,T., Henderson,D.M., Sifri,C.D. and
            Hendrickson,N.E.
  TITLE     Molecular characterization of the ldmdr1 multidrug resistance gene
            from Leishmani donovani
  JOURNAL   Unpublished (1992)
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     CDS             446..4471
                     /function="unknown"
                     /standard_name="multidrug-resistance P-glycoprotein"
                     /product="P-glycoprotein"
                     /gene="ldmdr1"
                     /evidence=EXPERIMENTAL
                     /codon_start=1
                     /translation="MPEGSETGASAKGGSVFEWEDRQGPFSPNQPIAEGLAERGDCGD
                     HNDYRCGSTSQMREVINMSHTYDVYVDEGKLEPVNRLAGQSESTHGGSSYSRHNPLFS
                     AKEEVKRAAVHESVSPIAIFRYADTADRVLMIVGTIFAVCSGAGTPLFSFIFGRIATD
                     LMSGVGSVELSAAKTSLIMVYVGIGMFVACGGHVLCWTVAASRQEGRIRLNFFRAVLR
                     QDIGWHDEHSPGELTARMTGDTRVIQNGINDKLSQGIMNGAMGIIGYIAGFVFSWDVT
                     LVMVGMMPFIIVMAAIIGNIVSKMTESSRKHFAKAGSLATEVMENIRTVQVFGREDYE
                     LQRFAEAVLYAQDRGIRKEFAGNLSAAVIMALVYLSYTIAFFFGSYLVEWGRRDMADI
                     ISTFLAVLMGSFGLGFVAPSATAFTESRAAAYEIFKAIDRVPPVDIDAGGVPVTGFKQ
                     SIEFRNVRFSYPTRPDMILFRDLNLTIKCGQKVAFSGSSGCGKSSMIGLIQRFYDPVG
                     GAVLCDGVDLRELCLHDWRDQIGIVSQEPNLFAGTMMENVRVGKPNATEEEVIEACRQ
                     ANVHDTIMSLPDQYNTPVGAVGSQLSGGQKQRIAIARALVKRPPVLLLDEATSALDRK
                     SEMEVQRSLDQLMQRGGMTVIVIAHRLATIRNVDCIYYVKYDGAEGSKITESGTFDEL
                     MELGGEFAAMARIQGVSTGDARSGARGQDGGKANDFLNAILDEAALAQLDEEAPRTAR
                     QKVPIEELAKWEVNGVSVGFRRLMGMNKDKSWAVALGILGSAVGGAARPTSTILMGYM
                     LRVLGEYSVNKNVEQLRSGTNLYAPLFIVFAVANFLGWILHGFYGYAGEHLTTKIRVL
                     LFRQIMRQDMNFFDIPGRDAGTLAGMLSGDCEAVHQLWGPSIGLKVQTVCIVASGIVV
                     SFIYQWKLALVALACMPLLIGCSLAERMMMNGYTKSKEGDTSDTIVTEALSSVRTVTS
                     FNMKADRVEAFEATLRVEVPRSVKKGIIAGSIYGATQFIYYSAYALCFWYGSKLIDRG
                     EAQFTDVMIASMSILFGAQNAGEAGAFATKLADAERSAKRVFSVIDRVPDVDIEQSGD
                     KDLGKGCDVDFRKVQFIYSARPKQVVLASVNLRFGDGMANGLMGQTGCGKSTIIQMLA
                     RFYDRRSGLICVNGKDLSSLDIAEWRRNISVVLQEPNLFSGTVRENIRYAREDATDEE
                     VEEAARLAHIHWEIMKWPDGYNTEVGYKGSALSGGQKQRVAIARGLLRRPKLLLLDEA
                     TSALDSVTEAKVQEGINVYQAKYGVTSVSIAHRLTTIRHCDQIILLDSGHIIEQGSHE
                     ELMALGGEYMMRYDLYTSASS"
                     /citation=[1]
BASE COUNT      898 a   1207 c   1563 g   1022 t
ORIGIN
        1 acgaggagac ggccggacaa gaggtgtgcc gctccttctc ttcctctttg cacgataccg
       61 cctctttcga cgcagctctt gttgctgttg cgtttttttt tcttcttcca ttgccgctcc
      121 ctctctctgt ggggagaggc acgtgtgcat cacctctcaa tttcgacccg attcggctct
      181 ttggtgtgtg tgtgtgtgtg tgtgtgtgtg gggcgccgtt gtaggctcgc ttgtcgccgt
      241 cgtctctctt tgtcgagcgc gcccagcgga agcgcgtcgc ccgcacgtcg actacccaca
      301 gacgaaagta acggacccga agctcatttc acccaggcca gcgtctttcc cctctttatt
      361 tcctctgctg actggggcgc acggaggaaa agtaccgact ttacgctaaa gactccagtt
      421 ttgtagcttg cacgctgtat acgccatgcc cgagggctct gaaactggtg cgagcgccaa
      481 gggcggctct gtctttgaat gggaggatag acaggggcca ttttctccta accaacccat
      541 cgcggagggg ctggcagaac gtggagactg tggcgatcac aatgactata gatgcggttc
      601 gacatcgcag atgagggagg tgatcaatat gtcgcacacg tatgacgtgt atgtggacga
      661 gggaaagctg gaaccggtga ataggcttgc cggccagagt gaaagcacgc acggcggcag
      721 cagctacagc aggcacaatc ctctgttcag cgccaaggag gaggttaaga gggcggcggt
      781 gcatgaaagt gtgagcccta tagcgatttt tcgctacgct gacacagctg accgcgttct
      841 catgattgtt ggcaccatct ttgcagtctg cagcggtgcc ggcacgcctt tgttctcttt
      901 tatatttggc cgtatcgcaa cagatctcat gtctggcgtg ggcagtgtgg agctcagcgc
      961 ggcgaagacg tcgttgatca tggtgtacgt cggtattggc atgtttgtgg cctgtggtgg
     1021 ccacgtgcta tgttggacgg tggcagcgtc tcgtcaggaa gggcgcatcc gtctgaactt
     1081 ctttcgcgct gtgctgcgcc aggacattgg atggcacgac gagcacagcc ctggcgagct
     1141 gaccgctcgc atgacgggcg acacacgtgt gattcagaat ggcattaacg acaagttgtc
     1201 gcagggtatc atgaacggcg ccatgggtat catcggctac atcgccggct tcgtgttttc
     1261 gtgggacgtg acgcttgtga tggtcggcat gatgcccttc attattgtca tggctgccat
     1321 aatcggcaac atcgtgtcca agatgacaga gtcgtcgcgc aagcactttg cgaaggccgg
     1381 atctctggcg actgaggtga tggagaacat ccgcacggtg caggtgtttg gccgggagga
     1441 ctacgagttg cagcggtttg ctgaagcggt cctgtacgcc caggaccgtg gtatccgcaa
     1501 agagtttgcg ggcaaccttt ctgcggcggt gatcatggcc ctcgtgtacc tcagctacac
     1561 catcgccttt ttcttcggct cctacttagt cgaatggggt cgccgtgata tggcggatat
     1621 catttctacc tttctagccg tgctgatggg tagctttggc ctcgggttcg tggcacccag
     1681 cgcgactgca ttcaccgagt cccgtgctgc cgcgtacgaa atcttcaagg cgatcgatcg
     1741 tgtaccaccg gtggacatcg acgctggcgg cgtgcctgta acgggcttta agcagagcat
     1801 cgagttccgc aacgtgcggt tctcgtaccc gactcgccca gacatgatac tgttccgcga
     1861 cctgaacctg acgatcaagt gcgggcagaa ggttgcgttc tctgggtcgt ctgggtgcgg
     1921 caagtcgtcg atgatcgggc tgatccagcg cttctacgac cctgttggcg gcgccgtgct
     1981 gtgcgacggc gtggacttgc gcgagctgtg cctgcacgac tggcgcgacc agatcgggat
     2041 cgtgtcgcag gagccgaacc tgttcgcggg gacgatgatg gagaacgtgc gcgtgggaaa
     2101 accgaacgcg acggaggagg aggtgatcga ggcctgcaga caggcgaacg tgcacgacac
     2161 catcatgagc ctgccggacc agtacaatac gcccgtgggt gctgtgggat cgcagctgtc
     2221 gggcgggcag aagcagcgaa tcgcgattgc gcgcgcactt gtgaagcggc cgccggtcct
     2281 gctgctggac gaggcgacga gcgcgctgga ccggaagtct gagatggagg tacagcgttc
     2341 actggaccag ctgatgcaga ggggcgggat gaccgtgatc gtgattgcgc accgactcgc
     2401 gacgatccga aacgtggatt gcatctacta cgtaaagtac gacggcgcgg aggggagcaa
     2461 gattacggag agcgggacgt ttgacgagct gatggagctc ggtggagagt tcgctgcgat
     2521 ggcgaggatt cagggcgtgt ctactggtga cgcgaggagc ggcgcaaggg ggcaggacgg
     2581 tggcaaggca aatgacttcc tgaacgcgat tctggacgag gcggctcttg cgcaactgga
     2641 cgaggaggcg ccgcgcacgg cgcgtcagaa ggtgccgatc gaggagctcg cgaagtggga
     2701 agtgaatggt gtgagcgttg gcttccggcg gttgatggga atgaataaag acaaatcgtg
     2761 ggctgtggct ctgggtattc tcggctcggc ggtgggtggt gccgcccgac ctacgagcac
     2821 catcttgatg ggctacatgc tgcgcgtgct tggcgagtac agtgttaaca agaatgttga
     2881 acaactacgc agcggaacca acctgtacgc tccgctgttt attgtctttg cggttgcaaa
     2941 cttcttgggt tggattctgc acggctttta cggctacgca ggtgagcacc tgacaacgaa
     3001 gatccgcgtg ttgctgttcc gtcagatcat gcgccaggac atgaacttct ttgacattcc
     3061 cggacgtgat gcgggcaccc tggctgggat gctgtccggc gactgcgagg ccgtgcatca
     3121 gctgtggggc ccgtcgattg gtctgaaagt gcagacggta tgcattgttg cttctggaat
     3181 tgtggtgagc ttcatctacc agtggaagct tgcgcttgtg gcgctggctt gcatgccgtt
     3241 gctcattggt tgcagccttg ctgagcgtat gatgatgaac gggtatacaa agagcaagga
     3301 gggtgacaca agcgacacga ttgtgacgga ggcgctgtcg agcgtgcgca cggtgacgtc
     3361 gttcaacatg aaggcggatc gcgtcgaggc cttcgaggca accctgcgag tagaggtgcc
     3421 gcgtagtgta aaaaagggca tcattgccgg cagcatctat ggcgccacac agtttattta
     3481 ttacagcgcc tacgcactgt gcttctggta tggtagcaaa ctgattgaca ggggcgaggc
     3541 gcaattcacg gatgtcatga tcgcgagcat gtcgatcctg ttcggtgcgc agaacgccgg
     3601 tgaagctggt gcgttcgcga cgaagctggc ggatgcagag cgctctgcga aacgtgtgtt
     3661 cagcgtgatc gaccgcgtgc ctgacgtgga catcgagcag tccggcgaca aggatctggg
     3721 taagggctgt gacgttgact tccgaaaagt gcagtttatc tattctgctc gaccgaagca
     3781 ggttgtgctt gcgtccgtga acctgcggtt cggcgacgga atggccaacg ggctgatggg
     3841 gcagacggga tgcggaaagt cgacgatcat ccagatgctt gcccgcttct acgatcggcg
     3901 ctctgggctg atctgcgtga acgggaagga cctgtcgtca ctggacatcg cggagtggcg
     3961 gcgcaacatc agtgttgtgc tccaggagcc gaatctgttc agcgggactg tgcgggagaa
     4021 catccgctac gcgcgcgagg acgcgacgga cgaggaagtg gaggaggccg cgcggcttgc
     4081 gcacatccac tgggagatca tgaagtggcc agatggctac aacacggaag tggggtacaa
     4141 aggcagcgcg ctatctggcg gtcagaagca gcgcgttgcg atcgctcgcg ggctgctgcg
     4201 gcgaccgaaa ctcctgctgc tggacgaggc gacgagcgcg ctcgacagtg tgacggaggc
     4261 gaaggtgcaa gagggcatca acgtgtacca ggcgaaatac ggggtcacgt cggtgagcat
     4321 tgcgcaccgg ttgacaacga tccgccactg cgaccagatc atcctgctag actctgggca
     4381 catcatcgag cagggcagcc acgaggagct gatggcactt ggcggggagt acatgatgcg
     4441 ctatgacctg tacacgagcg cgagctcgtg aggaaggctg ggcgccgcgc gactgcacat
     4501 gcccgcggcg gggctttgct cgttactgac ttttttgtgg cttgtttcta ccacggaggg
     4561 atcgtggttg ctttggggcg acgctttcgt gcgctcggct tcggtggcgc agctcttgca
     4621 tggcgccttt cgcttaccat acgcatcgcc gatgtcttgg gagccattag gggccacttg
     4681 tgttcccggg
//