From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Homo sapiens GM-CSF enhancer region.
Date: 29 Dec 92 00:45:22 GMT
Distribution: bionet
Checksum: 03778 5

LOCUS       HUMENHANCE    718 bp ds-DNA             PRI       03-DEC-1992
DEFINITION  Homo sapiens GM-CSF enhancer region.
ACCESSION   L07488
KEYWORDS    .
SOURCE      Homo sapiens DNA.
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 718)
  AUTHORS   Yang,Y.-C., Kovacic,S., Kriz,R., Wolf,S., Clark,S.C., Wellems,T.,
            Nienhuis,A. and Epstein,N.
  TITLE     The human genes for GM-CSF and IL3 are closely linked in tandem on
            chromosome 5
  JOURNAL   Blood 71, 958-961 (1988)
  STANDARD  full automatic
REFERENCE   2  (bases 1 to 718)
  AUTHORS   Cockerill,P.N., Shannon,M.F., Bert,A.G., Ryan,G.R. and Vadas,M.A.
  TITLE     The GM-CSF/IL3 locus is regulated by an inducible cyclosporin a
            sensitive enhancer
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. (1992) In press
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     enhancer        1..716
                     /standard_name="GM-CSF enhancer"
                     /evidence="EXPERIMENTAL"
                     /label=BglII_716bp
                     /citation=[2]
                     /note="716 bp BglII fragment 3.3 to 2.6 kb upstream of the
                     GM-CSFgene and 7 kb 3' of the IL3 gene, functions as an
                     induciblecyclosporin A sensitive enhancer required for
                     activation ofboth genes"
     protein_bind    158..192
                     /function="transcription factor"
                     /bound_moiety="NFAT"
                     /standard_name="NFAT"
                     /evidence="EXPERIMENTAL"
                     /label=GM170
                     /citation=[2]
     protein_bind    168..174
                     /function="TRANSCRIPTION FACTOR"
                     /bound_moiety="AP1"
                     /standard_name="FOS/JUN"
                     /evidence="EXPERIMENTAL"
                     /label=GM170
                     /citation=[2]
                     /note="STRONG BINDING SITE"
     protein_bind    315..343
                     /function="transcription factor"
                     /bound_moiety="NFAT"
                     /evidence="EXPERIMENTAL"
                     /label=GM330
                     /citation=[2]
     protein_bind    330..336
                     /function="transcription factor"
                     /bound_moiety="AP1"
                     /standard_name="fos/jun"
                     /evidence="EXPERIMENTAL"
                     /label=GM330
                     /citation=[2]
                     /note="strong binding site"
     protein_bind    396..402
                     /function="transcription factor"
                     /bound_moiety="AP1"
                     /standard_name="fos/jun"
                     /evidence="EXPERIMENTAL"
                     /label=GM400
                     /citation=[2]
                     /note="weak binding site"
     protein_bind    537..566
                     /function="transcription factor"
                     /bound_moiety="NFAT"
                     /evidence="EXPERIMENTAL"
                     /label=GM550
                     /citation=[2]
     protein_bind    545..552
                     /function="transcription factor"
                     /bound_moiety="AP1"
                     /standard_name="fos/jun"
                     /evidence="EXPERIMENTAL"
                     /label=GM550
                     /citation=[2]
                     /note="moderate binding site"
BASE COUNT      148 a    219 c    166 g    183 t      2 others
ORIGIN      
        1 bbagatctca ggtccccaga gataacaaga accctcatca agggttggac aggtcaaaga
       61 aggatcagat cagccccaaa gcctgtgcgg gggtgccatg gtaccgaagg actttgaggc
      121 ccatttcctt ccccttgtga taatgtctct cgtgataagg atcctggagt gactcaagcc
      181 cctgtttcct acagagcctg tggggtggac acgcatagga aactccttcc agagggtttt
      241 ctcctgtctc tgtaggaagg ggggccccag aggggtcata ccacccatcg tggtccccgc
      301 ctctgccctg ccacagcccc atcggagccc ctgagtcagc atggctggct atcggttgac
      361 actgtttaaa atacagtgca atctaggaat gcctgcctgt gtcatggcct cagccctgat
      421 gtcatctttc catgagaaag atgtgtggct gcccacaaac ctcatcacca aacccatgga
      481 tttctgtcct gtttgtgctg acttgggcac ctgcaggcca ccactgccga aatggatttc
      541 ttattatgac tcttgctttc ctcctttcct tccctcctag gcccttggcc actggcctgt
      601 tacttgccca tctgttatgt cccagccctt aaacacagct ttgctggccc tcagctcttc
      661 ttcctgaata acttccgtgg cctcagctac cacacagaca tgtaccgctg ccagatct
//