From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Zea mays tryptophan synthase beta-subunit (TSB2) mRNA, complete
Date: 31 Dec 92 01:39:09 GMT
Distribution: bionet
Checksum: 03652 5

LOCUS       MZEORP2A     1540 bp ss-mRNA            PLN       17-DEC-1992
DEFINITION  Zea mays tryptophan synthase beta-subunit (TSB2) mRNA, complete
            cds.
ACCESSION   M76685
KEYWORDS    TSB2 gene; tryptophan synthase beta-subunit.
SOURCE      Zea mays (library: W22 kernel library 9-1 of K.Cone) whole kernel,
            30 days after pollination cDNA to mRNA.
  ORGANISM  Zea mays
            Eukaryota; Plantae; Embryobionta; Magnoliophyta; Liliopsida;
            Commelinidae; Cyperales; Poaceae.
REFERENCE   1  (bases 1 to 1540)
  AUTHORS   Wright,A.D., Moehlenkamp,C.A., Perrot,G.H., Neuffer,M.G. and
            Cone,K.C.
  TITLE     The maize auxotrophic mutant orange pericarp is defective in
            duplicate genes for tryptophan synthase beta
  JOURNAL   Plant Cell 4, 711-719 (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     sig_peptide     3..137
                     /gene="TSB2"
                     /codon_start=1
     mat_peptide     138..1331
                     /product="tryptophan synthase beta-subunit"
                     /gene="TSB2"
                     /codon_start=1
     CDS             3..1334
                     /EC_number="4.2.1.20"
                     /standard_name="'orange pericarp-2/tryptophan synthase
                     beta-2'"
                     /gene="TSB2"
                     /evidence="EXPERIMENTAL"
                     /partial
                     /product="tryptophan synthase beta-subunit"
                     /codon_start=1
                     /translation="PGPPPPAPEGRRRRGRGRNAAGQAVAAEASPAAVEMGNGAAAPG
                     LQRPDAMGRFGRFGGKYVPETLMHALTELESAFHALATDDEFQKELDGILKDYVGRES
                     PLYFAERLTEHYKRADGTGPLIYLKREDLNHTGAHKINNAVAQALLAKRLGKQRIIAE
                     TGAGQHGVATATVCRRFGLQCIIYMGAQDMERQALNVFRMRLLGAEVRAVHSGTATLK
                     DATSEAIRDWVTNVETTHYILGSVAGPHPYPMMVREFHKVIGKETRRQAMDKWGGKPD
                     VLVACVGGGSNAMGLFHEFVEDQDVRLVGLEAAGHGVDTDKHAATLTKGQVGVLHGSM
                     SYLLQDDDGQVIEPHSISAGLDYPGVGPEHSFLKDIGRAEYDSVTDQEALDAFKRVSR
                     LEGIIPALETSHALAYLEKLCPTLADGVRVVVNCSGRGDKDVHTASKYLDV"
BASE COUNT      335 a    422 c    482 g    301 t
ORIGIN      41 cM.
        1 gtccaggacc tcctccgccc gcccccgaag gccgccgccg ccgtggccgc ggccgcaatg
       61 cagccggcca agcggtcgcc gcggaggcct cccccgccgc ggtggagatg gggaacggcg
      121 ccgcggcgcc ggggctgcag aggcccgacg ccatgggcag gttcgggagg ttcggcggca
      181 agtacgtccc cgagacgctc atgcacgcgc tcaccgagct cgagagcgcc ttccacgcgc
      241 tcgccaccga cgacgagttc cagaaagaac tggacggtat tctcaaggac tacgttggac
      301 gtgagagccc cctatacttc gcggagcgcc taacggagca ctacaagcgc gccgatggca
      361 caggcccgct catctacctc aagagggagg accttaacca cacgggtgcc cacaagatca
      421 acaatgccgt ggcgcaggcc ttgcttgcca agcgacttgg gaagcagcgc atcattgccg
      481 agacaggggc tggtcagcac ggcgtcgcca ccgccaccgt gtgccgccgt ttcgggctgc
      541 agtgcatcat ctacatgggc gcacaggata tggagaggca ggcgctcaat gttttccgga
      601 tgaggcttct tggtgcagag gtcagggcgg tacattctgg gacagcaacc ctgaaggatg
      661 caacttcaga ggctatccgt gactgggtca caaatgtgga gaccacacac tatatcttgg
      721 gctctgttgc cggtccgcac ccgtacccga tgatggtgag ggagttccat aaggttattg
      781 gcaaggaaac ccggaggcag gccatggaca agtggggtgg caagccggac gtattggttg
      841 cctgcgtcgg cggcggatca aacgccatgg gcttattcca tgagtttgtt gaggatcaag
      901 atgtgaggct ggttggactg gaggcagctg gccatggcgt agacaccgac aagcatgcgg
      961 cgactttgac aaagggccaa gtcggagttc tccatggatc catgagctat ttgctgcagg
     1021 atgatgacgg acaagttatc gaaccccact ccatcagtgc tgggctcgat taccctggcg
     1081 ttggacccga gcacagtttc ttgaaggaca ttggacgtgc cgaatacgat agtgtgactg
     1141 accaggaggc actggatgct ttcaagcgcg tgtctcggtt ggagggcatc atccctgccc
     1201 tggagacctc ccacgcgctg gcctaccttg agaagctctg cccgacactg gcggacggcg
     1261 tgagggtggt ggtgaactgc agcgggcgag gagacaagga cgtgcacacc gccagcaagt
     1321 acctggacgt ctaaaacacc agcgaggagg ctggtttcca tgtcaagttt tggagggttc
     1381 agcagtaatt aatttagcgc tgttcttttt ggataaaagc acaaataacc tggaagctgg
     1441 tattgttgtt gtagccggta gtgcccccct gtttttttat tttacaagta atctgaatgg
     1501 ctttggagtg cataatcaat aatcaaataa atacaaagct
//