HDTenEightyP/NetNews huggingface dataset:
From: ARABIDOPSIS@UK.AC.AFRC.JII
Newsgroups: bionet.genome.arabidopsis
Subject: Ad for 10th John Innes Symposium
Date: 22 Jul 92 15:53:00 GMT
Distribution: bionet
Original-To: ARAB-GEN@UK.AC.DARESBURY

ADVERTISEMENT FOR:

THE TENTH JOHN INNES SYMPOSIUM SEPTEMBER 7-10, 1992
"The Chromosome"

*** Places are still available.  Contact the Symposium 
Secretary -- address at the end of this message ***

SCIENTIFIC PROGRAMME

15TH BATESON MEMORIAL LECTURE: Professor Frank Stahl 
Genetic recombination:  thinking about it in phage and fungi.

LOWER ORDER STRUCTURE, ORIGINS OF REPLICATION AND 
PARTITIONING
C. Greider (Cold Spring Harbor) Telomeres and telomerase
M. DePamphilis (New Jersey, USA) Mammalian origins of DNA 
replication and their relationship to nucleosome segregation
G. Leno (Cambridge, UK) Assembly and replication of the 
Eukaryotic chromosome
M. Schaechter (Boston, USA) Equivalent of mitosis in bacteria
D.J. Sherratt (Glasgow, UK) Site-specific recombination and 
the partition of bacterial chromosomes

HIGHER ORDER STRUCTURES
C. Higgins (Oxford) The bacterial chromosome:  DNA 
supercoiling, chromatin structure and gene expression
J. Rouviere-Yaniv (Paris, France) The function of HU as a 
histone-like protein and its roles in vitro and in vivo
U.K. Laemmli (Geneva, Switzerland) Chromosome domains and 
gene expression
T. Cremer (Heidelberg) 2D and 3D-chromosome topography 
studied by combinatorial fluorescence in situ hybridization
P. Shaw (John Innes Institute, Norwich) Nuclear and nucleolar 
organisation in plants
L. Manuelidis (Yale, USA) DNA sequence motifs in chromosome 
organisation
P. Heslop-Harrison (John Innes Institute, Norwich) Genomes, 
chromosomes and the organisation of cell nuclei

EPIGENETICS
E. Richards (Cold Spring Harbor, USA) A. thaliana mutants with 
reduced levels of DNA methylation
N. Dillon (Mill Hill, London) The regulation of the human _-
globin domain
S. Henikoff (Seattle, USA) Cis- and trans-inactivation of 
Drosophila genes resulting from chromosome rearrangement
A. Ferguson-Smith (Cambridge, UK) Mechamism of genetic 
imprinting
P. Antequera (Edinburgh,UK) CpG islands and genes

KNOWLEDGE EMERGING FROM THE MAPPING & SEQUENCING OF 
GENOMES
E. Southern (Oxford) Horizons in genome analysis - a forward 
looking view
F. Blattner (Madison, USA) Sequencing the E. coli genome
R. Durbin (Cambridge, UK) Knowledge from the mapping and 
sequencing of the C. elegans genome
C. Dean (Cambridge Laboratory, Norwich) The Arabidopsis 
genome project
G. Moore (Cambridge Laboratory, Norwich) Genome analysis on 
small grain cereals
S.G. Oliver (Manchester, UK) The Great Wall:  sequencing the 
yeast genome
P. Goodfellow (Cambridge, UK) The meaning of gene order

Places are still available including 4 'assisted' places for UK 
students.

For further information please contact: Symposium Secretary, 
John Innes Institute, John Innes Centre, Norwich Research 
Park, Colney, Norwich, Norfolk, NR4  7UH Tel:  0603 52571  
Fax: 0603 56844  Telex:  975122 (JIINOR G)
From: elizabeth_earle@QMRELAY.MAIL.CORNELL.EDU ("Elizabeth Earle")
Newsgroups: bionet.genome.arabidopsis
Subject: Postdoctoral position avail
Date: 29 Jul 92 09:02:51 GMT
Distribution: bionet


                       Subject:                               Time:4:42 PM
  OFFICE MEMO          Postdoctoral position available        Date:7/29/92
A postdoctoral position is available this September in a USDA-funded project on
transformation of plant cells with yeast artificial chromosomes.  Close
interaction with other postdoctoral associates in the Cornell Plant Science
Center is planned.  Strong molecular biology experience is required; experience
with yeast is desirable but not required.  If interested, send CV, cover
letter, and names of 3 references to Dr. Lisa Earle, Dept. of Plant Breeding,
Cornell University, Ithaca, NY 14853-1902.  Questions about the position can be
directed  either to Dr. Earle (607-255-3102;
Elizabeth_Earle@qmrelay.mail.cornell.edu) or to Dr. Alan Blowers
(315-539-1200).
From: k.r.mitchelson@uk.ac.aberdeen
Newsgroups: bionet.genome.arabidopsis
Subject: subscription to the Arabidopsis bb
Date: 25 Jul 92 12:12:49 GMT
Distribution: bionet
Original-To: arab-gen@uk.ac.daresbury

name: Keith Mitchelson
address: dept. of molecular & cell biology,
university of aberdeen,
aberdeen, AB9 1AS, UK
e-mail: k.r.mitchelson@uk.ac.aberdeen
From: jwillis@OREGON.UOREGON.EDU
Newsgroups: bionet.genome.arabidopsis
Subject: (none)
Date: 22 Jul 92 00:39:00 GMT
Distribution: bionet

Does anyone on the Arabidopsis network know if any inversion stocks have
been constructed?  I am particularly interested to find out if any balancer
stocks exist.

Thanks in advance,
John Willis
jwillis@oregon,uoregon.edu
From: JMBOYCE@UK.AC.OXFORD.VAX
Newsgroups: bionet.genome.arabidopsis
Subject: stereo images
Date: 22 Jul 92 09:32:00 GMT
Distribution: bionet
Original-To: ARAB-GEN@UK.AC.DARESBURY

I would like to find software that transforms data points with 3-dimensional
coordinates into 2-dimensional stereo pairs.  Can anyone suggest programs and
where to get them - preferably for free.  We need something that will handle
about 25 points but more would be even better.

Thanks

Joy Boyce
FR*ATLAS*CICT; arrival Fri, 24 Jul 92 14: 50:46 +0100 action Relayed
From: CAMPBELL@EARN.FRCICT81
Newsgroups: bionet.genome.arabidopsis
Subject: Eucalyptus codon usage
Date: 24 Jul 92 13:50:46 GMT
Distribution: bionet
P1-Message-Id: FR*ATLAS*CICT; 920724143555519-CICT-TLSE
Original-To: arab-gen@UK.AC.DARESBURY, methods-and-reagents@UK.AC.DARESBURY,
        plantbio@UK.AC.DARESBURY
Ua-Content-Id: 920724143555519-

Fellow netters,
 
First my apologies for cross-postings - sorry for the clutter, I just want
to reach some interested party in as wide an audience as possible.
 
Now to the question:
 
Recently I have established a codon usage table for Eucalyptus based on
a small number of genes that have been cloned in this lab from
Eucalyptus gunnii.  I am interested in determining whether the codon usage
data that I have can be extended to other Eucalyptus species and therefore
was wondering if anyone out there with Eucalyptus sequnce data would be
willing to share their codon usage data.  I can compile a more
general Eucalyptus table and distribute it to interested parties if
any responses are received.  I'm interested in using the data to generate
better oligo probes based on amino acid sequence data that we have in the
lab.
 
I realize there aren't a whole lot of groups working on Eucalyptus molecular
biology out there so, if you know of any (particularly if they aren't in the
net), could you please bring this posting to their attention.
 
Thanks in advance,
 
Malcolm
 
Malcolm Campbell, Ph.D.
Equipe lignines
Centre de Physiologie Vegetale
Universite Paul Sabatier
Toulouse, FRANCE
 
CAMPBELL@FRCICT81.BITNET
 
Usual disclaimers...
 
Potatoe, potatto
Tomatoe, tomatto
Let's Quail the whole thing off
From: jwillis@OREGON.UOREGON.EDU
Newsgroups: bionet.genome.arabidopsis
Subject: inverions/balancers
Date: 22 Jul 92 00:42:00 GMT
Distribution: bionet

Does anyone on the Arabidopsis network know if any inversion stocks have
been constructed?  I am particularly interested to find out if any balancer
stocks exist.

Thanks in advance,
John Willis
jwillis@oregon,uoregon.edu
From: bbaker@GARNET.BERKELEY.EDU
Newsgroups: bionet.genome.arabidopsis
Subject: postdoc position
Date: 30 Jul 92 20:17:21 GMT
Distribution: bionet

postdoctoral positions
university of california, berkeley / usda
plant gene expression center

postdoctoral positions available immediately: (1) for transposon
insertional mutagenesis in arabidopsis thaliana, and (2) for
positional cloning and characterization of the tmv resistance 
locus, n, of nicotiana tabacum.

applicants should have a ph.d., background in molecular biology, 
and experience in recombinant dna techniques.  experience in 
genetics is preferred.

interested applicants should send a curriculum vitae, names of 
and addresses of three references, and a brief description of 
research interests by september 1,1992 to:

dr. barbara baker 
plant gene expression center
800 buchanan st.
albany, ca 94710

university of california, berkeley and its affiliated institutions 
are equal opportunity and affirmative action employers.
From: liuz@STARBASE1.CALTECH.EDU ("LiuZ")
Newsgroups: bionet.genome.arabidopsis
Subject: RE: transformant seeds request
Date: 22 Jul 92 02:35:11 GMT
Distribution: bionet


_______________________________________________________________________________

Dear Tim Caspar

I am in Dr. Meyerowitz's lab at Caltech.  I am using truncated 35S promoter/GUS
 constructs for an enhancer trapping experiment and would like to get some of
your 35S/GUS transgenic plant seeds as control.
My address: Zhongchi Liu, Division of Biology 156-29, Caltech, Pasadena, 
            CA 91125

Thank you in advance for your help

Zhongchi Liu
From: braam@RICE.EDU (Janet Braam)
Newsgroups: bionet.genome.arabidopsis
Subject: RNA synthesis inhibitors
Date: 27 Jul 92 19:59:31 GMT
Reply-To: braam@rice.edu
Distribution: bionet

Does anyone know if there are transcription 
inhibitors that work well in plant cells?  
(I hear that Actinomycin D is problematic)

Thanks in advance for any advice!
Janet Braam
From: JIM2@PSUVM.PSU.EDU ("June Medford")
Newsgroups: bionet.genome.arabidopsis
Subject: meristem GUS expression in Arabidopsis
Date: 21 Jul 92 13:17:00 GMT
Distribution: bionet

Tim Caspar requested any transgenic Arabidopsis expressing GUS in the shoot
or root apical meristems.  My lab has several transgenic lines expressing
the meri-5 promoter fused to GUS.  Meri-5 is preferentially expressed in the
shoot meristem, early in development and then shows expression in the
vascular tissues and recently initiated organs.  This work and the meri
genes were described in a paper in the Plant Cell (Medford et al 3:359-370).
The meri-5 promoter work was at Monsanto, although I made the transgenic
Arabidopsis here.  Folks wanting the seed need to first get permission from
Monsanto (contact Dr. Harry Klee-- Monsanto Co, 700 Chesterfield Village
Parkway, St.  Louis MO 63198).  Please note that we should have other shoot
meristem GUS lines available in the future that will not have the same
Monsanto restrictions.  I'm sorry if this is an inconvience to anyone.
Also, please note that the meri-5 construct expresses in theshoot meristem
and since the root meristem is less well defined in Arabidopsis,I prefer to
describe it's expression as "at or near the root meristem".  Please email me
if there's any questions.  June Medford.
Newsgroups: bionet.genome.arabidopsis
From: kerrylee@athena.mit.edu (Kerry Lee Tucker)
Subject: Devastating Infestation
Keywords: Unknown Bugs 
Organization: Massachusetts Institute of Technology
Date: Mon, 27 Jul 1992 17:06:08 GMT

Dear Net-folk:

   Does anyone have experience with a small spider (under 1 mm in length) of
tan color which builds webs along the flowering bolts of A. thaliana and 
amongst the flowers?  Infested plants suffer yellow discoloration followed by
wilting and slow death.  This bug is wreaking havoc in our plant room and
soon will destroy everything!!  If you know how to handle the scourge,
please respond to me at kerrylee@athena.mit.edu
  Panicked, K. L. Tucker
From: jjc4@PO.CWRU.EDU (James J. Campanella)
Newsgroups: bionet.genome.arabidopsis
Subject: Arabidopsis and biolistic transformation
Date: 22 Jul 92 11:34:06 GMT
Reply-To: jjc4@po.CWRU.Edu
Distribution: bionet



Dear network,

has anyone attempted transformation of Arabidopsis using the

biolistics approach? Have you had any success? Are your protocols

at all different from the standard protocols used with soybeans and

tobacco? 

--
Jim Campanella|"Iron Man I, how do you copy?"
CWRU Bio Dept.|"With a Xerox machine. And you?"
Cleveland,    |  -Tom Servo, Marooned, MST3K
Ohio          |
From: NANTEL@UNCVX1.BITNET
Newsgroups: bionet.genome.arabidopsis
Subject: Subscription
Date: 30 Jul 92 18:27:00 GMT
Distribution: bionet

PLease add my name to the mailing list for the Arabidopsis bulletin board

Andre Nantel
Dept. of Biology
University of North Carolina-Chapel Hill

NANTEL@UNCVX1.ACS.UNC
Xref: sparky bionet.biology.tropical:68 bionet.agroforestry:92
From: tamone@uni2a.unige.ch
Newsgroups: bionet.biology.tropical,bionet.agroforestry
Subject: Plumeria hazardous in Mediterranea?
Date: 30 Jul 92 09:41:52 GMT
Organization: University of Geneva, Switzerland

Hi there,

Could someone tell me if there is a hazard of any kind in introducing
a plant called Plumeria in the Mediterranean region (Switzerland and
southern Italy).

I don't know the gender and specie of the plant. It comes from my
trip to Hawaii where it is sold for exportation. 

The plant gives a bush with white and scented flowers (tough it can be
of other color too).


I would like to know if the plant already exists in the region I want to
put it. I don't want to upset the local flora !


Thanks if you have any info on that plant.

Francois
Xref: sparky bionet.biology.tropical:67 bionet.population-bio:109
From: jstravis@bass.bu.edu (John Travis)
Newsgroups: bionet.biology.tropical,bionet.population-bio
Subject: environmental science careers
Date: 28 Jul 92 17:43:40 GMT
Reply-To: jstravis@bass.bu.edu (John Travis)
Followup-To: bionet.biology.computational
Distribution: bionet
Organization: Boston University

Sunce this worked well for me, my colleague will try a posting....

attenion environmental scientists

My name is Richard Stone and I'm a staffwriter at Science Magazine. The 
reason I'm hailing you is to ask for help with an article on careers in
environmental careers. But I'm hung on a few minor details:

--how do you define "environmental science"
--in what durection is the field moving
--where are the jobs? inn industry? governement? academia?
--are there subdisciplines in envir. science that are vastly
undersatffed or overstaffed.

I'm interested in hearing your answers to these and any others questions
I've neglected to ask. please call me ASAP at 202-326-6593 or fax me reply
at 202-371-9227. You can also email a friend of mine at
 jstravis@bu-pub.bu.edu

Thanks for the help and feel free to circulate this among your colleagues
Xref: sparky bionet.biology.tropical:66 bionet.agroforestry:90
From: una@phy.duke.edu (Una Smith)
Newsgroups: bionet.biology.tropical,bionet.agroforestry
Subject: NGOs on Internet or via gateways
Summary: List of NGOs with e-mail available, please send additions
Keywords: NGO Asia Africa Latin America e-mail CONSLINK
Date: 27 Jul 92 01:40:37 GMT
Followup-To: bionet.biology.tropical
Organization: Duke University Physics Dept.; Durham, N.C.


The following announcement and request for information
was recently  distributed on the CONSLINK subscription list.
Please reply directly to Michael Stuewe, whose e-mail and
surface address are included at the bottom of this message.

	Una

PS, I have lightly edited the formatting of Michael's text
to satisfy my newsreader's verbosity daemon, which won't
let me send this out unless I put in as many lines of text
as there are lines of included text...

...is this enough yet?  


> As some of you may know CONSLINK provides a list of NGOs
> active in conservation on its bulletin board. You can
> request that list by sending a message to
> LISTSERV@SIVM.BITNET which contains only the line: 

> GET CONSLINK NGOS

> We are interested in compiling a list of NGOs especially
> in Latin America, Asia and Africa which can be reached by
> electronic mail. Please let us know of NGOs you know
> and/or any already compiled lists you are aware off. 

>           Michael Stuewe, Conservation Center, National Zoo
>           Smithsonian Institution, Front Royal, VA 22630, USA
>           Telephone: 703-635-6542 E-mail: NZPEM001@SIVM.BITNET

-- 

Una Smith   una@phy.duke.edu    School of the Environment
                                Duke University
                                Durham, NC  27706
From: mrigank@aiims.ernet.in
Newsgroups: bionet.biology.computational
Subject: Re: Gaps and PAMs
Date: 30 Jul 92 03:57:50 GMT
Distribution: bionet
Originator: biocomp@lerkim.umiacs.umd.edu

Hello
I am looking for energy parameters for following to be used 
in Energy minimization. I have sets of AMBER GROMOS and SCHERRAGA
So compatible with either will do. We can fine tune also. Actuallu its
for metal in the proteins like
if Me is metal then 
1.	Its paarmeters for Lennard Jones 6-12 potential

2.	C-alpha-ME, N-Me, S-Me, O-Me etc. 

3.	X-Me-X and Me-X-Me angle parameters

4.	X-Me-X-X dihedral angle parameter.

mainly for Me = Zn, Ca, Fe, Cu

Thanks

Dr.Mrigank,Ph.D
Deptt. Of Biophysics
AIIMS, New Delhi 110 029

e-mail vikram!aiims!mrigank@shakti.ernet.in
From: gburges@ccu.umanitoba.ca (Garry Burgess)
Newsgroups: bionet.biology.computational
Subject: Datalogger Interfacing
Date: 21 Jul 92 16:32:03 GMT
Distribution: bionet
Organization: University of Manitoba, Winnipeg, Canada
Originator: biocomp@lerkim.umiacs.umd.edu


We are using a Macintosh Powerbook 140, running Soft PC, to run the
software that comes with the Campbell Scientific  CR10 Measurement and
control module (DataLogger), but are having some problems making the
connection between the computer and the datalogger.

Is there anyone with any pinout recommendations for the cable to link
these two devices, or who could advise us on an inexpensive cable solution
to this problem.


Garry
From: toms@ncifcrf.gov (Tom Schneider)
Newsgroups: bionet.biology.computational
Subject: Re: Are viruses alive?
Date: 23 Jul 92 20:59:50 GMT
Distribution: bionet
Organization: Frederick Cancer Research and Development Center
Originator: biocomp@lerkim.umiacs.umd.edu


In article <CMM.0.90.2.711650592.pkarp@Rockaway.AI.SRI.COM> pkarp@ai.sri.com
(Peter Karp) writes:

|Harold Morowitz' forthcoming book, "Metabolism Recapitulates
|Biogenesis: The Beginnings of Cellular Life" has a succinct definition
|of living systems.  To paraphrase from a draft of the book, an
|autonomous biological self-replicating system is a molecular
|self-replicating entity that is capable of evolving, and that operates
|in the absence of other self-replicating entities.  Therefore, a virus
|is not an autonomous biological self-replicating system because it
|requires the presence of other self-replicating entities to replicate.
|Whether it is alive or not depends on whether you equate life with an
|autonomous biological self-replicating system, or simply a biological
|self-replicating system.

Hmm.  So humans must not be "autonomous biological self-replicating systems"
because we depend on oxygen, vitamin C and various amino acids.  But I think
that most people would claim to be alive (or object violently if someone else
claimed that they were not!) so we must drop the "autonomous" in our general
definition.  Anyway, the only creatures that are fully autonomous are
prototrophic bacteria that can use simple minerals to build everything they
need.

Ok, now that we've established that viruses are "alive" (because you can't draw
a line!) what about small pox?  It will be destroyed completely in a few years;
it is only in certain labs now.  But wait!  A mad scientist resynthesizes the
genetic material from a GenBank entry 100 years from now, and everybody gets
infected.  Was it alive while in GenBank?

  Tom Schneider
  National Cancer Institute
  Laboratory of Mathematical Biology
  Frederick, Maryland  21702-1201
  toms@ncifcrf.gov
From: dangold1@iastate.edu (Daniel M Goldman)
Newsgroups: bionet.biology.computational
Subject: Re: Mapmaker
Date: 24 Jul 92 13:20:25 GMT
Distribution: bionet
Organization: Iowa State University, Ames IA
Originator: biocomp@lerkim.umiacs.umd.edu

In <1992Jul23.131204.28093@news.iastate.edu> tabq7@isuvax.iastate.edu writes:

>Does anyone know where I can find a copy of the MSDOS version of Mapmaker?

>Thanks,

>Phil

Phil-If you find such a version, please let me know.  We use mapmaker on
     the Vincent system and would be interested in cross validating the
     results.  Thanks -DmG  515-294-9369 or 3052 (msgs) or 9359 (FAX).
From: mark@uucp.nibr
Newsgroups: bionet.biology.computational
Subject: Mackey-Glass
Date: 29 Jul 92 08:29:46 GMT
Distribution: bionet
Originator: biocomp@lerkim.umiacs.umd.edu


I don't have a direct answer or solution to the Mackey-Glass equation,
but you could loop up the analysis of Murray in his book:

 J.D. Murray, Mathematical Biology, 1989 (ISBN 3-540-19460).

He treats the equation on page 15-19, deriving some simplifications
and solutions.

Mark van Veen
mark%nibr@nluug.nl
From: tabq7@isuvax.iastate.edu
Newsgroups: bionet.biology.computational
Subject: Mapmaker
Date: 23 Jul 92 13:12:04 GMT
Distribution: bionet
Organization: Iowa State University, Ames, Iowa
Originator: biocomp@lerkim.umiacs.umd.edu

Does anyone know where I can find a copy of the MSDOS version of Mapmaker?

Thanks,

Phil
From: agapow@latcs1.lat.oz.au (paul-michael agapow)
Newsgroups: bionet.biology.computational
Subject: Re: Are viruses alive?
Date: 23 Jul 92 03:36:26 GMT
Distribution: bionet
Organization: Comp Sci, La Trobe Uni, Australia
Originator: biocomp@lerkim.umiacs.umd.edu

pkarp@ai.sri.com (Peter Karp) writes:
>Harold Morowitz' forthcoming book, "Metabolism Recapitulates
>Biogenesis: The Beginnings of Cellular Life" has a succinct definition
>of living systems.  To paraphrase from a draft of the book, an
>autonomous biological self-replicating system is a molecular
>self-replicating entity that is capable of evolving, and that operates
>in the absence of other self-replicating entities.  Therefore, a virus
>is not an autonomous biological self-replicating system because it
>requires the presence of other self-replicating entities to replicate.
>Whether it is alive or not depends on whether you equate life with an
>autonomous biological self-replicating system, or simply a biological
>self-replicating system.

Might i point out that this excludes all forms of sexually reproducing
life, since they are incapable of evolving without another form of life
(of the opposite sex) to reproduce with.

Nonetheless, Morowitz's definition is a brave attempt - i've just been
tangling with the definition problem and evntually concluded that the
problem was best handled if reduced to :

"X may not be alive, but for the purposes of discussion, we may regard it
as being such, or acting exactly as if it is ..."

This avoids a lot of the sticky problems involved in the philosophy of the
problem. Examining Morowitz's definition again, it strikes me that he
places a heavy (perhaps too heavy) emphasis on evolution. There seems to
be two problems here : firstly defining what an entity is (where does it
start and finish, how is it distinct from its environ and other entities),
and the separation of evolution from other behaviours. Strictly speaking
we could treat evolution as the adaptation of the "meta-organism", the
ancestral line of organisms. This itself gives rise to two interesting
questions - does an organism have to evolve to be alive or just adapt?
Does a virus adapt?

Hmmm,

p-m

--------------------------------------------------------------------------------
paul-michael agapow (agapow@latcs1.oz.au) Machine Intelligence Lab, LaTrobe Uni

        "Teenage sex, nuclear winter, Junior Miss fashion 
               & the mechanics of emotional capitalism ..."
From: pda@dcs.ed.ac.uk (Paul Adams)
Newsgroups: bionet.biology.computational
Subject: CM at EPCC
Date: 21 Jul 92 16:34:27 GMT
Distribution: bionet
Organization: Edinburgh Parallel Computing Centre, University of Edinburgh
Originator: biocomp@lerkim.umiacs.umd.edu


There have been some questions about the type of CM at Edinburgh. It is in
fact a CM-200 running at 8MHz. Hope this clears up any confusion.

   Paul Adams