From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Bacteroides uniformis ORF2, complete cds; beta-lactamase (cblA)
Date: 10 Jan 93 05:45:25 GMT
Distribution: bionet
Checksum: 25734 6

LOCUS       BNRCBLAX     2787 bp ds-DNA             BCT       09-JAN-1993
DEFINITION  Bacteroides uniformis ORF2, complete cds; beta-lactamase (cblA)
            gene, complete cds.
ACCESSION   L08472
KEYWORDS    beta-lactamase; cblA gene; homologous region.
SOURCE      Bacteroides uniformis (individual_isolate WAL7088) DNA.
  ORGANISM  Bacteroides uniformis
            Prokaryotae; Gracilicutes; Scotobacteria; Anaerobic gram-negative
            straight, curved and helical rods; Bacteroidaceae.
REFERENCE   1  (bases 1 to 2787)
  AUTHORS   Smith,C.J., Parker,A.C. and Bennett,T.K.
  TITLE     Cloning and nucleotide sequence of a chromosomal beta-lactamase
            from Bacteroides uniformis
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     CDS             163..654
                     /note="putative;  homologous to E. coli envD gene; ORF2"
                     /codon_start=1
                     /translation="MDFGSLSGDAGGTTTTVVFVMALLVAFLVLAAQYESWTSPVAAV
                     MGLPVALLGAMLGCYVMGTPVSIYTQIGIILLVALSAKNGILIVEFARDFRAQGNSIR
                     DAHSRPDISPATYSDDIACIRIRGYAAAFCNRSRGGKPYCTGSRRSVRYGTEYLACHG
                     LYS"
     CDS             844..1734
                     /gene="cblA"
                     /evidence="EXPERIMENTAL"
                     /product="beta-lactamase"
                     /codon_start=1
                     /translation="MKAYFIAILTLFTCIATVVRAQQMSELENRIDSLLNGKKATVGI
                     AVWTDKGDMLRYNDHVHFPLLSVFKFHVALAVLDKMDKQSISLDSIVSIKASQMPPNT
                     YSPLRKKFPDQDFTITLRELMQYSISQSDNNACDILIEYAGGIKHINDYIHRLSIDSF
                     NLSETEDGMHSSFEAVYRNWSTPSAMVRLLRTADEKELFSNKELKDFLWQTMIDTETG
                     ANKLKGMLPAKTVVGHKTGSSDRNADGMKTADNDAGLVILPDGRKYYIAAFVMDSYET
                     DEDNANIIARISRMVYDAMR"
BASE COUNT      752 a    671 c    667 g    697 t
ORIGIN      
        1 cccgggtgga agagcagtta ggcatggacc agataaacag atataacatg tactccacag
       61 cagccgtgac ctgcaatgtg gctccgggca gtagttcggg agagggtatc ggcaaatgga
      121 aagtctaatc aaagagtatc tcggagacga gttcggctat gaatggactt cggtagccta
      181 tcaggagacg caggcggaac cacgacgact gtcgtattcg tcatggcact gctggttgcc
      241 ttccttgtac tcgccgcaca atacgaaagc tggaccagtc cggtagctgc cgtaatggga
      301 cttccggttg cactgctcgg tgccatgttg ggatgctatg tcatggggac tccggtcagc
      361 atctacacac aaattggaat cattctgctt gtcgccttgt ctgccaaaaa cggtattctg
      421 attgtggagt ttgcacgtga ctttcgtgca caaggcaatt caatccgcga tgcgcattcc
      481 aggccggaca tatcgcctgc gacctattct gatgacatcg cttgcattcg tattcggggt
      541 tatgccgctg ctttttgcaa ccggagcagg ggcgggaagc cgtattgcac tgggagccgc
      601 cgtagtgttc ggtatggcac tgaatacctt gcttgccacg gtctatattc ctaatttcta
      661 cgaattgatg cagaagttgc aggagaagtt cagtaagaaa caatagggag gatgagctac
      721 aagttatgga ttatgggtta taagctatca cacttatagc ccataatcca taactcattt
      781 tagcaacttg ctgctaaaag ttgtatcttt acgtgcgaat tgcctcttaa agaaattgaa
      841 gttatgaaag catatttcat cgccatactt accttattca cttgtatagc taccgtcgtc
      901 cgggcgcagc aaatgtctga acttgaaaac cggattgaca gtctgctcaa tggcaagaaa
      961 gccaccgttg gtatagccgt atggacagac aaaggagaca tgctccggta taacgaccat
     1021 gtacacttcc ccttgctcag tgtattcaaa ttccatgtgg cactggccgt actggacaag
     1081 atggataagc aaagcatcag tctggacagc attgtttcca taaaggcatc ccaaatgccg
     1141 cccaatacct acagccccct gcggaagaag tttcccgacc aggatttcac gattacgctt
     1201 agggaactga tgcaatacag catttcccaa agcgacaaca atgcctgcga catcttgata
     1261 gaatatgcag gaggcatcaa acatatcaat gactatatcc accggttgag tatcgactcc
     1321 ttcaacctct cggaaacaga agacggcatg cactccagct tcgaggctgt ataccgcaac
     1381 tggagtactc cttccgctat ggtccgacta ctgagaacgg ctgatgaaaa agagttgttc
     1441 tccaacaagg agctgaaaga cttcttgtgg cagaccatga tagatactga aaccggtgcc
     1501 aacaaactga aaggtatgtt gccagccaaa accgtggtag gacacaagac cggctcttcc
     1561 gaccgcaatg ccgacggtat gaaaactgca gataatgatg ccggcctcgt tatccttccc
     1621 gacggccgga aatactacat tgccgccttc gtcatggact catacgagac ggatgaggac
     1681 aatgcgaaca tcatcgcccg catatcacgc atggtatatg atgcgatgag atgagagagg
     1741 caagggggct attggtagct cctcctataa gttttgatac tcgggtatat cctgcagaaa
     1801 agtatagctg ttgtgctcgt aatctatgcg gcaacaataa tgctgaagcc cgttgctgac
     1861 aatcagataa tccactttca gcaccatgtt gtagcgggta atctggtcga aaaccttttg
     1921 ggtgatttct atttcaggcg ccttgtattc cacaatcatc cgggcggtaa ggtcgcgtcg
     1981 atagagcact gtatcacagc gtttctttgt accgttcagc tgtacctgca cctcatttgc
     2041 cataagcgcc tgcggatacc ccttgtgagc aagaagaaag tggacgaagt gctgccgcac
     2101 ccattcttca ggggtcaagg cgacataacg acgacggatt acatcgaaaa taacattttt
     2161 tccgttcctt gcggcaattt tagcatcgaa tcgtggtagg tttaacgata acatttacta
     2221 catttgtaag ttaaataatg gttccgtctt ttgggaacac aaatttaatg aaatcttatg
     2281 aaaacaaaag aagaaatcgt agccaattgg ctgccccgtt acacaaagcg taacctggag
     2341 gattttggag agtacattct gttgaccaac ttcaacaagt acgtagagat tttcgcagaa
     2401 aagttcaatg ttcccattct cggtaaagat gccaacatga tatctgccag tgcagaagga
     2461 atgaccatca ttaattttgg tatgggaagt cctaatgccg ccattatcat ggacttgctg
     2521 agtgctatca agcccaaggc atgtctgttt ctgggtaagt gcggcggtat agacaagaaa
     2581 aatagaatag gggacctcat ccttcccatt gccgccatcc gtggcgaggg tacctccaac
     2641 gactacttcc cacctgaagt cccgtcactc ccggcattta tgctgcaacg tgccgtttct
     2701 tctgccatcc gcgattatgc ccgtgactac tggaccggta ccgtatacac caccaaccgc
     2761 cgcatttggg aacatgacga ggaattc
//