From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Entamoeba histolytica NADP+-dependent alcohol dehydrogenase (ADH1)
Date: 26 Jan 93 05:47:12 GMT
Distribution: bionet
Checksum: 09599 4

LOCUS       ENHADH1A     1125 bp ss-mRNA            INV       25-JAN-1993
DEFINITION  Entamoeba histolytica NADP+-dependent alcohol dehydrogenase (ADH1)
            mRNA, complete cds.
ACCESSION   M88600
KEYWORDS    NADP+-dependent alcohol dehydrogenase.
SOURCE      Entamoeba histolytica (strain HM-1:IHSS) cDNA to mRNA.
  ORGANISM  Entamoeba histolytica
            Eukaryota; Animalia; Protozoa; Sarcomastigophora; Sarcodina;
            Rhizopoda; Lobosa; Gymnamoeba; Schizopyrenida; Tubulina;
            Entamoebidae.
REFERENCE   1  (bases 1 to 1125)
  AUTHORS   Kumar,A., Shen,P.-S., Descoteaux,S., Pohl,J., Bailey,G. and
            Samuelson,J.
  TITLE     Cloning and expression of an NADP(+)-dependent alcohol
            dehydrogenase gene of Entamoeba histolytica
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 89, 10188-10192 (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     misc_binding    174..179
                     /bound_moiety="nucleotide binding GxGxxG"
                     /gene="ADH1"
     polyA_site      1125
                     /gene="ADH1"
     CDS             19..1101
                     /EC_number="1.1.1.2"
                     /gene="ADH1"
                     /note="NADP+ and zinc-dependent protein"
                     /product="alcohol dehydrogenase"
                     /codon_start=1
                     /translation="MKGLAMLGIGRIGWIEKKIPECGPLDALVRPLALAPCTSDTHTV
                     WAGAIGDRHDMILGHEAVGQIVKVGSLVKRLKVGDKVIVPAITPDWGEEESQRGYPMH
                     SGGMLGGWKFSNFKDGVFSEVFHVNEADANLALLPRDIKPEDAVMLSDMVTTGFHGAE
                     LANIKLGDTVCVIGIGPVGLMSVAGANHLGAGRIFAVGSRKHCCDIALEYGATDIINY
                     KNGDIVEQILKATDGKGVDKVVIAGGDVHTFAQAVKMIKPGSDIGNVNYLGEGDNIDI
                     PRSEWGVGMGHKHIHGGLTPGGRVRMEKLASLISTGKLDTSKLITHRFEGLEKVEDAL
                     MLMKNKPADLIKPVVRIHYDDEDTLH"
BASE COUNT      405 a    148 c    252 g    320 t
ORIGIN      
        1 gcacgaggaa aaaccacaat gaaaggactt gctatgcttg gaattggaag aattggatgg
       61 attgaaaaga aaatcccaga atgtggacca cttgatgcat tagttagacc attagcactt
      121 gcaccatgta catcagatac acataccgtt tgggcaggag ctattggaga tagacatgat
      181 atgattcttg gacatgaagc ggttggacaa attgttaaag ttggatcatt agttaagaga
      241 ttaaaagttg gagataaagt tattgtacca gctattacac cagattgggg agaagaagaa
      301 tcgcaaagag gatatccaat gcattcagga ggaatgcttg gaggatggaa attctcaaat
      361 ttcaaggatg gagttttttc agaagttttc catgttaatg aagcagatgc caatcttgca
      421 cttcttccaa gagatattaa accagaagat gcagttatgt tatcagatat ggtaactact
      481 ggattccatg gagcagaatt agctaatatt aaacttggag atactgtttg tgttattggt
      541 attggaccag ttggattaat gtcagttgca ggagcaaacc atcttggagc aggaagaatc
      601 tttgcagtag gatcaagaaa acattgttgt gatattgcat tggaatatgg agcaacagat
      661 attattaatt ataaaaatgg agatattgta gaacaaattc ttaaagctac agacggcaaa
      721 ggagttgata aagtcgttat tgcaggaggt gatgttcata catttgcaca agcagtcaaa
      781 atgattaaac caggatcaga tattggaaat gttaattatc ttggagaagg agataatatt
      841 gatattccaa gaagtgaatg gggagttgga atgggtcata aacacattca tggaggttta
      901 accccaggtg gaagagtcag aatggaaaaa ttagcatcac ttatttcaac tggtaaatta
      961 gatacttcta aacttattac acatagattt gaaggattag aaaaagttga agatgcatta
     1021 atgttaatga agaataaacc agcagacctt atcaaaccag ttgtcagaat tcattatgat
     1081 gatgaagata ctcttcatta aattcattaa ttcaaagtat taaac
//