HDTenEightyP/NetNews huggingface dataset:
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human chromosome 4 sequence-tagged site STS4-131
Date: 21 Aug 92 10:06:47 GMT
Distribution: bionet
Checksum: 50966     3

LOCUS       HUM4STS131    419 bp ds-DNA             PRI       21-AUG-1992
DEFINITION  Human chromosome 4 sequence-tagged site STS4-131
ACCESSION   M95240
KEYWORDS    STS; human chromosome 4; sequence tagged site.
SOURCE      Homo sapiens, clone C4-88 from Los Alamos National Laboratory
            chromosome 4 cosmid library, plate 4-1-1R, pos 10C; vector SuperCos
            1 (Stratagene)
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 419)
  AUTHORS   Goold,R.D., diSibio,G., Dugaiczyk,A., Smith,K.A., Xu,H.,
            Magrane,G., Lang,D.B., Cox,D.R., Masters,S.B. and Myers,R.M.
  TITLE     Sequence-Tagged Sites for Human Chromosome 4
  JOURNAL   Unpublished (1992) see COMMENT for author address
  STANDARD  full automatic
COMMENT     PCR components: 25 ng of human genomic DNA, 10 pmol of each
            oligonucleotide, 200 micro-M dNTPs, 0.25 U Taq  polymerase (Cetus)
            in 10 micro-l of 50 mM KCl-20 mM  Tris-HCl, pH 8.3 (at room temp),
            2.5 mM MgCl-2.  Initial denaturation at 94degC for 1.5 min, then 30
            cycles of  94degC for 15 sec, 62degC for 23 sec, and 72degC for 30
            sec, followed by a final extension at 72degC for 3.5 min, using a
            Perkin-Elmer 9600 thermocycler. PCR-amplified product size 209 bp.
            Sequence submitted by:
            Human Genome Mapping Center
            Box 0925
            University of California San Francisco
            San Francisco, CA 94143-0925  USA
            Phone: (415) 502-1612   Fax: (415) 476-8391
            e-mail: hgmcprobes@cgl.ucsf.edu
FEATURES             Location/Qualifiers
     misc_feature    34..55
                     /label=Primer1
     misc_feature    complement(219..242)
                     /label=Primer2
BASE COUNT       96 a     82 c    109 g     94 t     38 others
ORIGIN
        1 tggggggggc cgnggcanct tccaccaggc ttcacctccc acactgggaa ccacatttta
       61 acatgagatt tggaggggac agacgttcan accgtatcaa acatgaaact gcatttcgca
      121 tcctaccata aagccttgtt ttggggtgag gaagtggctg taagtgaaga atgactgcct
      181 cgtttctggt ttggacaact gcattggaat gtctgtggga catggaattg gtcatacagg
      241 tgtagaacct aagacaggtg gcctcggctg gagaaatann tcagnaggtg gtcgnngnag
      301 ccatggnatt agntgagttt cctcgnctcg natacagcct naananggag cataaaacan
      361 natcncnngg tatcccncan gacttgtntn ttgngggnng nnnctnntgn ngntccnan
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Hamster ribosomal protein L7 mRNA.
Date: 15 Aug 92 10:01:27 GMT
Distribution: bionet
Checksum: 12275     2

LOCUS       CRUL7RP       187 bp ss-mRNA            ROD       15-AUG-1992
DEFINITION  Hamster ribosomal protein L7 mRNA.
ACCESSION   M99687 M89804
KEYWORDS    ribosomal protein L7.
SOURCE      Cricetulus griseus ovary cDNA to mRNA.
  ORGANISM  Cricetulus griseus
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Rodentia; Myomorpha; Cricetidae; Cricetinae; Cricetini.
REFERENCE   1  (bases 1 to 187)
  AUTHORS   Zhu,W., Keng,P. and Chou,W.-G.
  TITLE     Differential gene expression in wild type and X-ray sensitive
            mutants of Chinese hamster ovary cell lines
  JOURNAL   Unpublished (1992)
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     mRNA            59..118
                     /note="K19 mRNA codes ribosomal protein L7 and is
                     underexpressed in the xrs-5 and xrs-6 mutants of the CHO
                     cell line"
                     /gene="K19"
                     /evidence=EXPERIMENTAL
                     /citation=[1]
                     /partial
BASE COUNT       50 a     54 c     48 g     35 t
ORIGIN
        1 cggacgagca ggaggccaag aaggtggtga accctttgtt tgagaagagg cctaagaact
       61 tcggcattgg gcaggacatc cagcccaaac gagacctcac acgcttcgtc aaatggcccc
      121 gatacattcg gctgcagcgc aaagggccat cctctataag cgcttaaagt tcctcctgcc
      181 attacca
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human chromosome 4 sequence-tagged site STS4-58
Date: 21 Aug 92 10:11:07 GMT
Distribution: bionet
Checksum: 57699     3

LOCUS       HUM4STS58     398 bp ds-DNA             PRI       21-AUG-1992
DEFINITION  Human chromosome 4 sequence-tagged site STS4-58
ACCESSION   M95313
KEYWORDS    STS; human chromosome 4; sequence tagged site.
SOURCE      Homo sapiens, clone L4-39 from the C25 lambda library (Pritchard,
            Genomics 4, 408, 1989); vector - Lambda Dash II (Stratagene)
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 398)
  AUTHORS   Goold,R.D., diSibio,G., Dugaiczyk,A., Smith,K.A., Xu,H.,
            Magrane,G., Lang,D.B., Cox,D.R., Masters,S.B. and Myers,R.M.
  TITLE     Sequence-Tagged Sites for Human Chromosome 4
  JOURNAL   Unpublished (1992) see COMMENT for author address
  STANDARD  full automatic
COMMENT     PCR components: 25 ng of human genomic DNA, 40 pmol of each
            oligonucleotide, 200 micro-M dNTPs, 0.25 U Taq  polymerase (Cetus)
            in 10 micro-l of 50 mM KCl-20 mM  Tris-HCl, pH 8.3 (at room temp),
            2.5 mM MgCl-2.  Initial denaturation at 94degC for 1.5 min, then 30
            cycles of  94degC for 15 sec, 64degC for 23 sec, and 72degC for 30
            sec, followed by a final extension at 72degC for 3.5 min, using a
            Perkin-Elmer 9600 thermocycler. PCR-amplified product size 283 bp.
            Sequence submitted by:
            Human Genome Mapping Center
            Box 0925
            University of California San Francisco
            San Francisco, CA 94143-0925  USA
            Phone: (415) 502-1612   Fax: (415) 476-8391
            e-mail: hgmcprobes@cgl.ucsf.edu
FEATURES             Location/Qualifiers
     misc_feature    31..54
                     /label=Primer1
     misc_feature    complement(291..313)
                     /label=Primer2
BASE COUNT       99 a     78 c     79 g    130 t     12 others
ORIGIN
        1 tttnggngcc ctcaacggat cttnccttga catctggtcc ctggaaggaa tattaatatt
       61 tgatccccca ttccactttt angtttgggt tagagctgac ctgagctagt tccttttttc
      121 tgttcctgta aaattctatt tnattatctt gttctctttc taacctatgg catgagtctt
      181 gggggctacc catnttttag tttnctgtng ctgttgtaag aaattacaac acacataatg
      241 gcttaaaaca acacaaattt atcaagatct tagaaggcat caaccctcag tcctgatctt
      301 gagctgggaa gattttcctg gattatctgt gcagtnatca caagggtctt cataaaggga
      361 gtcagnagng ncagagtcag aaaggtctga ggatgcta
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human Ig germline H-chain gene V region, partial cds.
Date: 22 Aug 92 10:00:42 GMT
Distribution: bionet
Checksum: 20920     2

LOCUS       HUM8IGVH      434 bp ds-DNA             PRI       22-AUG-1992
DEFINITION  Human Ig germline H-chain gene V region, partial cds.
ACCESSION   M99404
KEYWORDS    immunoglobulin heavy chain subgroup VH-III;
            immunoglobulin heavy chain variable region.
SOURCE      Homo sapiens (individual_isolate LS) blood DNA.
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 434)
  AUTHORS   Adderson,E.E., Carroll,W.L. and Shackelford,P.G.
  TITLE     The human VH3b gene subfamily is highly polymorphic
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     CDS             join(1..13,117..217)
                     /product="immunoglobulin heavy chain variable region"
                     /gene="IGHV@"
                     /codon_start=1
                     /translation="AILKGVQCEVQLVESGGGLVKPGGSLRLSCAASGFTFS"
                     /partial
     sig_peptide     1..13
                     /gene="IGHV@"
                     /codon_start=1
                     /partial
     intron          14..116
                     /gene="IGHV@"
BASE COUNT      114 a     85 c    133 g    102 t
ORIGIN
        1 gctattttaa aaggtgattt atggagaact agagagatta agtgtgagtg gacgtgagtg
       61 agagaaacag tggatatgtg tggcagtttc tgatcttagt gtctctgtgt ttgcaggtgt
      121 ccagtgtgag gtgcagctgg tggagtctgg gggaggcttg gtaaagcctg gggggtccct
      181 tagactctcc tgtgcagcct ctggattcac tttcagtaac gcctggatga gctgggtccg
      241 ccaggctcca gggaaggggc tggagtgggt cggccgtatt aaaagcaaaa ctgatggtgg
      301 gacaacaaac tacgctgcac ccgtgaaagg cagattcacc atctcaagag atgattcaaa
      361 aaacacgctg tatctgcaaa tgaacagcct gaaaaccgag gacacagccg tgtattactg
      421 taccacagac acag
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Mus musculus Ig heavy chain mRNA V-region, from hybridoma M195.
Date: 14 Aug 92 10:00:58 GMT
Distribution: bionet
Checksum: 06528     3

LOCUS       MUSIGHM195    405 bp ss-mRNA            ROD       14-AUG-1992
DEFINITION  Mus musculus Ig heavy chain mRNA V-region, from hybridoma M195.
ACCESSION   M83098
KEYWORDS    immunoglobulin; immunoglobulin heavy chain; variable region.
SOURCE      Mus musculus hybridoma M195 cDNA to mRNA.
  ORGANISM  Mus musculus
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Rodentia; Myomorpha; Muridae; Murinae.
REFERENCE   1  (bases 1 to 405)
  AUTHORS   Co,M., Avdalovic,N., Caron,P.C., Avdalovic,M.V., Scheinberg,D.A.
            and Queen,C.
  TITLE     Chimeric and humanized antibodies with specificity for the CD33
            antigen
  JOURNAL   J. Immunol. 148, 1149-1154 (1992)
  STANDARD  full staff_entry
FEATURES             Location/Qualifiers
     sig_peptide     1..60
                     /gene="IgH"
                     /note="putative"
                     /codon_start=1
     CDS             1..405
                     /note="V-region"
                     /product="immunoglobulin heavy chain"
                     /gene="IgH"
                     /note="putative"
                     /codon_start=1
                     /translation="MGWSWIFLFLLSGTAGVHSEVQLQQSGPELVKPGASVKISCKAS
                     GYTFTDYNMHWVKQSHGKSLEWIGYIYPYNGGTGYNQKFKSKATLTVDNSSSTAYMDV
                     RSLTSEDSAVYYCARGRPAMDYWGQGTSVTVSS"
                     /partial
     mat_peptide     61..405
                     /note="V-region"
                     /product="immunoglobulin heavy chain"
                     /gene="IgH"
                     /codon_start=1
BASE COUNT      101 a    106 c    105 g     93 t
ORIGIN
        1 atgggatgga gctggatctt tctcttcctc ctgtcaggaa ctgcaggcgt ccactctgag
       61 gtccagcttc agcagtcagg acctgagctg gtgaaacctg gggcctcagt gaagatatcc
      121 tgcaaggctt ctggatacac attcactgac tacaacatgc actgggtgaa gcagagccat
      181 ggaaagagcc ttgagtggat tggatatatt tatccttaca atggtggtac tggctacaac
      241 cagaagttca agagcaaggc cacattgact gtagacaatt cctccagcac agcctacatg
      301 gacgtccgca gcctgacatc tgaggactct gcagtctatt actgtgcaag agggcgcccc
      361 gctatggact actggggtca aggaacctca gtcaccgtct cctca
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Rhesus macaque polyoma virus minor structural protein VP2 (5' end)
Date: 22 Aug 92 10:01:48 GMT
Distribution: bionet
Checksum: 58136     3

LOCUS       SV4VP2AP      574 bp ds-DNA             VRL       22-AUG-1992
DEFINITION  Rhesus macaque polyoma virus minor structural protein VP2 (5' end)
            and agnoprotein (complete cds) genes.
ACCESSION   M99346
KEYWORDS    agnoprotein; enhancer; minor structural protein VP2.
SOURCE      Rhesus macaque polyomavirus (strain A2895) (library: ATCC VR-305)
            DNA.
  ORGANISM  Rhesus macaque polyomavirus
            Viridae; ds-DNA nonenveloped viruses; Papovaviridae;
            Polyomaviruses.
REFERENCE   1  (bases 1 to 574)
  AUTHORS   Ilyinskii,P.O., Daniel,M.D., Horvath,C. and Desrosiers,R.C.
  TITLE     Genetic analysis of SV40 from brain and kidney of macaque monkeys
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     enhancer        complement(107..178)
                     /note="72-bp repeat"
     repeat_unit     complement(179..191)
                     /note="identical to nucleotides 215-227 of this clone.
                     Corresponds to nucleotides 251-263 in SV40 strain 776"
     variation       complement(179..227)
                     /note="replaces 2nd 72-bp repeat (nucleotides 179-250) of
                     SV40 strain 776"
     enhancer        complement(192..214)
                     /note="2nd 72bp repeat (partial)"
     repeat_unit     complement(215..227)
                     /note="identical to nucleotides 179-191 of this clone.
                     Corresponds to nucleotides 251-263 in SV40 strain 776"
     CDS             299..487
                     /product="agnoprotein"
                     /codon_start=1
                     /translation="MVLRRLSRQASVKVRRSWTESKKTARRLFVFVLELLLQFCEGED
                     TVDGKRKKPERLTEKPES"
     variation       replace(375,"a")
                     /note="corresponds to nucleotide 411 in SV40 strain 776"
     CDS             526..574
                     /product="minor structural protein VP2"
                     /codon_start=1
                     /translation="MGAALTLLGDLIATVS"
                     /partial
BASE COUNT      138 a    124 c    158 g    154 t
ORIGIN
        1 gcctcggcct ctgcataaat aaaaaaaatt agtcagccat ggggcggaga atgggcggaa
       61 ctgggcggag ttaggggcgg gatgggcgga gttaggggcg ggactatggt tgctgactaa
      121 ttgagatgca tgctttgcat acttctgcct gctggggagc ctggggactt tccacaccct
      181 aactgacaca cggagcctgg ggactttcca caccctaact gacacacatt ccacagctgg
      241 ttctttccgc ctcagaaggt acctaaccaa gttcctcttt cagaggttat ttcaggccat
      301 ggtgctgcgc cggctgtcac gccaggcctc cgttaaggtt cgtaggtcat ggactgaaag
      361 taaaaaaaca gctcgacgcc tttttgtgtt tgttttagag cttttgctgc aattttgtga
      421 aggggaagat actgttgacg ggaaacgcaa aaaaccagaa aggttaactg aaaaaccaga
      481 aagttaactg gtaagtttag tctttttgtc ttttatttca ggtccatggg tgctgcttta
      541 acactgttgg gggacctaat tgctactgtg tctg
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human immunodeficiency virus type 2 (FOENVC2) envelope protein
Date: 18 Aug 92 10:01:53 GMT
Distribution: bionet
Checksum: 56156     2

LOCUS       HIVENVC2      453 bp ss-RNA             VRL       18-AUG-1992
DEFINITION  Human immunodeficiency virus type 2 (FOENVC2) envelope protein
            fragment.
ACCESSION   M87102
KEYWORDS    envelope protein.
SOURCE      Human immunodeficiency virus type 2 uncultured blood mononuclear
            cells (3/90 sample) RNA.
  ORGANISM  Human immunodeficiency virus type 2
            Viridae; ss-RNA enveloped viruses; Positive strand RNA virus;
            Retroviridae; Lentivirinae.
REFERENCE   1  (bases 1 to 453)
  AUTHORS   Gao,F., Yue,L., White,A.T., Pappas,P.G., Barchue,J., Hanson,A.P.,
            Greene,B.M., Sharp,P.M., Shaw,G.M. and Hahn,B.H.
  TITLE     Human infection by genetically-diverse SIVsm-related HIV-2 in West
            Africa
  JOURNAL   Nature (1992) In press
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     misc_feature    1..453
                     /note="PCR derived HIV-2 envelope fragment"
                     /gene="env"
                     /note="putative"
                     /citation=[1]
BASE COUNT      190 a     76 c     76 g    111 t
ORIGIN
        1 aacgtggtca aaagacaaca aaaattgttg cgactaaccg tctgggagac taaaaacctc
       61 cagactaaag tcactgctat caaaaagtac ctaaagaacc aggcacagct aaattcatag
      121 gaatgcgcgt tcagacaggt ctgccacacc actgtaccat ggccaaataa tagtctcaca
      181 cctaactgaa acaatataac ttggcagaag tagaaaagaa aggttaattt cctaaaggca
      241 aatataacac agatgttaaa gaaagcacaa attcaacagg agaaaaacat gtataagtta
      301 caaaaactaa atagctagaa tgtatttggc aactggttta accttacttc ttaaattaag
      361 tatatacagt ataaagtctt catagtaata ggtataatat tgttaaaaat aataatctat
      421 gtagtacaaa tgttagctaa gttaagtaag gga
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Hepatitis C virus gene for envelope protein E 1
Date: 15 Aug 92 10:01:58 GMT
Distribution: bionet
Checksum: 28745     3

LOCUS       HPCVEPE1      533 bp ss-RNA             VRL       15-AUG-1992
DEFINITION  Hepatitis C virus gene for envelope protein E 1
ACCESSION   X67299
KEYWORDS    envelope protein.
SOURCE      Hepatitis C virus RNA.
  ORGANISM  Hepatitis C virus
            Viridae; ss-RNA enveloped viruses; Positive strand RNA virus;
            Togaviridae incertae sedis.
REFERENCE   1  (sites)
  AUTHORS   Hoehne,M., Schreier,E., Fuchs,K., Wiese,M., Deinhardt,F. and
            Roggendorf,M.
  TITLE     Variability of E 1 gene region of Hepatitis C virus in patients
            infected with contaminated immunoglobulin
  JOURNAL   Unpublished (1992)
  STANDARD  full staff_review
REFERENCE   2  (bases 1 to 533)
  AUTHORS   Schreier,E.
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
COMMENT     *source: clone=Glob. 1; *source: isolate=immunoglobulin/78;
            From EMBL    entry HCVEPE1;  dated 04-AUG-1992.
FEATURES             Location/Qualifiers
     CDS             <1..>533
                     /product="HCV polyprotein"
                     /codon_start=2
                     /translation="VSGVYHVTNDCFNSSIVYETADMIMHTPGCVPCVREDNSSRCWV
                     ALTPTLAARNGSVPTTAIRRHVDLLVGAAAFSSAMYVGDLCGSVFLVSQLFTLSPRRH
                     ETVQECNCSIYPGHVTGHRMAWDMMMNWSPTTALVVSQLLRIPQAVVDMVAGAHWGVL
                     AGLAYYSMVGNWAKVLI"
     mat_peptide     1..533
                     /product="envelope protein E1"
                     /codon_start=2
BASE COUNT       92 a    156 c    156 g    129 t
ORIGIN
        1 cgtgtccggg gtgtaccatg tcacgaacga ctgtttcaac tcaagcattg tgtatgagac
       61 agcggacatg atcatgcaca cccccgggtg cgtgccctgc gttcgggagg ataactcctc
      121 tcgctgctgg gtagcgctca cccccacgct cgcggctagg aatggcagtg tccccactac
      181 ggcgatacga cgccacgtcg atttgctcgt tggggcggct gctttctcgt ccgctatgta
      241 cgtgggggat ctttgcggat ctgttttcct cgtctcccag ctgttcaccc tctctcctcg
      301 ccggcatgag acagtacagg aatgtaattg ctcaatctac cccggccacg tgacaggtca
      361 ccgcatggct tgggatatga tgatgaactg gtctcctaca acagccctag tggtatcgca
      421 gttactccgg atcccacaag ctgtcgtgga catggtggcg ggggcccact ggggcgtcct
      481 ggcgggcctc gcctactatt ccatggtggg gaactgggct aaggttttga ttg
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: H.sapiens polymorphic microsatellite (DXS559)
Date: 15 Aug 92 10:02:17 GMT
Distribution: bionet
Checksum: 14489     2

LOCUS       HUMMS559      335 bp ds-DNA             PRI       15-AUG-1992
DEFINITION  H.sapiens polymorphic microsatellite (DXS559)
ACCESSION   X66054
KEYWORDS    microsatellite DNA.
SOURCE      Homo sapiens DNA.
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 335)
  AUTHORS   Lindsay,S.J.
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
COMMENT     *source: stage=adult; *source: cell_line=Thy-B-33-12 (human X-mouse
            hybrid); *source: clone=HX28; *source: chromosome=Xq12-q13;
            From EMBL    entry HSMS559;  dated 06-AUG-1992.
FEATURES             Location/Qualifiers
     satellite       119..166
                     /note="poly CA"
                     /evidence=EXPERIMENTAL
BASE COUNT       86 a    108 c     70 g     71 t
ORIGIN
        1 gatccttcac cactgcctcc actctgtgaa tcatctcttt cttgctcaag ggagagcacc
       61 ctcaattaat ttgattccca agcaggtgca tgcaggggca tgctcatgca ggcgcgcgca
      121 cacacacaca cacacacaca cacacacaca cacacacaca cacacactaa ccttgaatgg
      181 tatctgcatt tctactgtct ttgtctattc aacacccagt ctcttggcga tgggagcagg
      241 gagaacctac acacacctcc tcactggaat cggaatctgt gcccgaatct ggggcccacc
      301 cagtcgagga ttccggctga aggtagcggg cctag
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human chromosome 4 sequence-tagged site STS4-168
Date: 21 Aug 92 10:07:13 GMT
Distribution: bionet
Checksum: 36755     3

LOCUS       HUM4STS168    454 bp ds-DNA             PRI       21-AUG-1992
DEFINITION  Human chromosome 4 sequence-tagged site STS4-168
ACCESSION   L00738
KEYWORDS    STS; human chromosome 4; sequence tagged site.
SOURCE      Homo sapiens, clone p4-106 from a chromosome 4 plasmid library;
            vector pBluescript II KS + (Stratagene)
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 454)
  AUTHORS   Goold,R.D., diSibio,G., Dugaiczyk,A., Smith,K.A., Xu,H.,
            Magrane,G., Lang,D.B., Cox,D.R., Masters,S.B. and Myers,R.M.
  TITLE     Sequence-Tagged Sites for Human Chromosome 4
  JOURNAL   Unpublished (1992) see COMMENT for author address
  STANDARD  full automatic
COMMENT     PCR components: 25 ng of human genomic DNA, 10 pmol of each
            oligonucleotide, 200 micro-M dNTPs, 0.25 U Taq  polymerase (Cetus)
            in 10 micro-l of 50 mM KCl-20 mM  Tris-HCl, pH 8.3 (at room temp),
            2.5 mM MgCl-2.  Initial denaturation at 94degC for 1.5 min, then 30
            cycles of  94degC for 15 sec, 62degC for 23 sec, and 72degC for 30
            sec, followed by a final extension at 72degC for 3.5 min, using a
            Perkin-Elmer 9600 thermocycler. PCR-amplified product size 156 bp.
            Sequence submitted by:
            Human Genome Mapping Center
            Box 0925
            University of California San Francisco
            San Francisco, CA 94143-0925  USA
            Phone: (415) 502-1612   Fax: (415) 476-8391
            e-mail: hgmcprobes@cgl.ucsf.edu
FEATURES             Location/Qualifiers
     misc_feature    118..141
                     /label=Primer1
     misc_feature    complement(249..273)
                     /label=Primer2
BASE COUNT      125 a    110 c     92 g    123 t      4 others
ORIGIN
        1 nggggggggc caaaagtggt accgggcccc ccctcgaggt cgacggtatc gataagcttg
       61 atatcgaatt cctgcagccc acgccgtgaa tagacttccc actacatttt ttattttact
      121 ctccacctgt aaaagctaca attctcatgt ctaccatggg aaaatagggc acattcttca
      181 aagttcaact caaaagccgt tttctacttg agctctttgg caaacattaa gctggatgtc
      241 actttcttcc ttcaaacttc tctagcatta ttgtgggtcc aaaaagctcg ctttcagcac
      301 ctgtcgtttc ctttctttgc agagggtatt ttaaataaaa acattaagtt atgacgaaga
      361 agaacggaaa cgccttaaac cggaaaattt tcataaatag cgaaaacccg cgaggtcgcc
      421 gccccgnaac aaggnggatc attgngcttc gatg
//