From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human (clones SW11-1 and cKi-ras2-76) cellular transforming
Date: 31 Jul 92 10:01:39 GMT
Distribution: bionet
Checksum: 40203     6

LOCUS       HUMRASK06     617 bp ds-DNA             PRI       31-JUL-1992
DEFINITION  Human (clones SW11-1 and cKi-ras2-76) cellular transforming
            c-Ki-ras2 proto-oncogene, exon 4b and complete major and minor cds.
ACCESSION   L00049 K00652 K00653
KEYWORDS    K-ras oncogene; c-myc oncogene; proto-oncogene; ras oncogene;
            transforming gene; viral homology.
SEGMENT     6 of 6
SOURCE      Homo sapiens (library: Lawn et al.) colon DNA; and Homo sapiens
            (library: Lawn et al.) colon and lung cDNA to mRNA.
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 159 to 275)
  AUTHORS   Shimizu,K., Birnbaum,D., Ruley,M.A., Fasano,O., Suard,Y.,
            Edlund,L., Taparowsky,E., Goldfarb,M. and Wigler,M.
  TITLE     Structure of the Ki-ras gene of the human lung carcinoma cell line
            Calu-1
  JOURNAL   Nature 304, 497-500 (1983)
  STANDARD  full staff_review
REFERENCE   2  (bases 1 to 617)
  AUTHORS   McGrath,J.P., Capon,D.J., Smith,D.H., Chen,E.Y., Seeburg,P.H.,
            Goeddel,D.V. and Levinson,A.D.
  TITLE     Structure and organization of the human Ki-ras proto-oncogene and a
            related processed pseudogene
  JOURNAL   Nature 304, 501-506 (1983)
  STANDARD  full staff_review
REFERENCE   3  (bases 158 to 275)
  AUTHORS   Capon,D.J., Seeburg,P.H., McGrath,J.P., Hayflick,J.S., Edman,U.,
            Levinson,A.D. and Goeddel,D.V.
  TITLE     Activation of Ki-ras2 gene in human colon and lung carcinomas by
            two different point mutations
  JOURNAL   Nature 304, 507-513 (1983)
  STANDARD  full staff_review
REFERENCE   4  (bases 159 to 275)
  AUTHORS   McCoy,M.S., Bargmann,C.I. and Weinberg,R.A.
  TITLE     Human colon carcinoma Ki-ras2 oncogene and its corresponding
            proto-oncogene
  JOURNAL   Mol. Cell. Biol. 4, 1577-1582 (1984)
  STANDARD  full staff_review
COMMENT     Approximately 1% of the mRNAs generated from this gene have the
            exon 4a variant stucture containing the sequence 0-1-2-3-4a-4b with
            exon 4b spliced onto 4a 5 bp after the exon 4a stop codon. The
            other 99% of the mRNAs generated have no exon 4a in their sequence;
            exon 4b is spliced directly onto exon 3.
            See comment for segment 1 of this entry.
            Complete source information:
            Human DNA (genomic library of Lawn et al.) [2]; human cDNA to colon
            (SW480) and lung (Calu-1) carcinoma cell lines mRNA, multiple
            clones [3]; human lung carcinoma cell line Calu-1 DNA [1]; human
            colon carccinoma cell line SW480, cDNA to mRNA, clone SW11-1, and
            human cell line G637, cDNA to mRNA, clone cKi-ras2-76 [4]
FEATURES             Location/Qualifiers
     CDS             join(L00045:96..206,L00046:358..536,L00047:299..458,
                     L00048:600..716,159..275)
                     /note="minor product"
                     /product="cellular transforming proto-oncogene"
                     /gene="c-Ki-ras2"
                     /codon_start=1
                     /translation="MTEYKLVVVGAGGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQV
                     VIDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHHYREQIKR
                     VKDSEDVPMVLVGNKCDLPSRTVDTKQAQDLARSYGIPFIETSAKTRQRVEDAFYTLV
                     REIRQYRLKKISKEEKTPGCVKIKKCIIMGVDDAFYTLVREIRKHKEKMSKDGKKKKK
                     KSKTKCVIM"
     CDS             join(L00045:96..206,L00046:358..536,L00047:299..458,
                     159..275)
                     /note="major product"
                     /product="cellular transforming proto-oncogene"
                     /gene="c-Ki-ras2"
                     /codon_start=1
                     /translation="MTEYKLVVVGAGGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQV
                     VIDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHHYREQIKR
                     VKDSEDVPMVLVGNKCDLPSRTVDTKQAQDLARSYGIPFIETSAKTRQGVDDAFYTLV
                     REIRKHKEKMSKDGKKKKKKSKTKCVIM"
     intron          order(L00048:724..>866,<1..158)
                     /number=E
                     /note="5.3 kb gap"
                     /gene="c-Ki-ras2"
     exon            159..>275
                     /number=4b
                     /note="c-Ki-ras2 protein, b variant (see comment)"
                     /gene="c-Ki-ras2"
BASE COUNT      201 a     84 c    102 g    230 t
ORIGIN
        1 gaattctaaa agtcctaata tatgtaatat atattcagtt gcctgaagag aaacataaag
       61 aatcctttct taatattttt tccattaatg aaatttgtta cctgtacaca tgaagccatc
      121 gtatatattc acattttaat actttttatg tatttcaggg tgttgatgat gccttctata
      181 cattagttcg agaaattcga aaacataaag aaaagatgag caaagatggt aaaaagaaga
      241 aaaagaagtc aaagacaaag tgtgtaatta tgtaaataca atttgtactt ttttcttaag
      301 gcatactagt acaagtggta atttttgtac attacactaa attattagca tttgttttag
      361 cattacctaa tttttttcct gctccatgca gactgttagc ttttacctta aatgcttatt
      421 ttaaaatgac agtggaagtt tttttttcct cgaagtgcca gtattcccag agttttggtt
      481 tttgaactag caatgcctgt gaaaaagaaa ctgaatacct aagatttctg tcttggggtt
      541 tttggtgcat gcagttgatt acttcttatt tttcttacca agtgtgaatg ttggtgtgaa
      601 acaaattaat gaagctt
//