From: zhilkin@peter.ibd.nrc.ca (Peter Zhilkin)
Subject: Molecular Biology/Protein Analysis. Position wanted
Original-To: biojobs@uk.ac.daresbury
Distribution: bionet
Date: Fri, 22 Jan 1993 17:45:20 GMT


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  PhD in molecular biology. 15 years of experience in investigating
  structure-function organization of proteins, analysis of antigenic
  structure and computer design of peptides, proteins with improved
  properties and artificial proteins which are candidates for vaccines.
  Many theoretical developments were successfully confirmed by experi-
  ments. Development of original methods for analysis of proteins based
  on their amino acid sequences. Experience with different software
  packages for analysis of protein and nucleic acid sequence and pro-
  tein structure: sequence alignment, phylogenetic analysis, comparison
  of sequences with databases, secondary structure prediction, functi-
  onal and antigenic site searching, etc.

			Curriculum Vitae
			-----------------

Name:                     Alexei Maksimovich Eroshkin

Date of birth:            January 12, 1954, Novosibirsk

Citizenship: 		  Russia

Marital status:           Married

Command of English:       Speak fluently, read and write

Current address:          Koltsovo 1/62, Novosibirsk Region
                          633159  Russia

Contact information:	  Tel: +7 (3832) - 64 7774 work
			       +7 (3832) - 64 7616 home
			  Telex: 133196 NPO SU
			  Fax: +7 (3832) - 328831
			  E.mail: root@cgi.nsk.su
				(via Streletc Victor)

Education:
 Institution and Location       Degree    Year    Field

 Novosibirsk State University   M.S.      1976    Applied
  (Novosibirsk, Russia)                           mathematics

 Research Institute             Ph.D      1986    Molecular
 of Molecular Biology,                            biology
 NPO "Vector", (Novosibirsk
 region, Russia)

Topic of Ph.D thesis:   Theoretical approaches for the design of
			proteins with modified properties.

Employment and research experience :
 Research Institute of Molecular Biology,
 NPO "Vector"  Novosibirsk Region, Russia

   1976-1977            Postgraduate Research Fellow.
			Fundamentals of biochemistry, biophysics and
			molecular biology.

   1977-1981            Junior Researcher, Researcher.
			Development of programs for simulation of mole-
			cular dynamics and energy minimization of macro-
			molecular systems.
			Dynamics of trypsin inhibitor and DNA fragments.

   1981-1986            Senior Researcher.
			Development of different methods and computer
			programs for analysis of protein sequences and
			structures (antigenic structure prediction,
			immunochemical cross-reaction estimation,
			study of evolutionary amino acid replacements,
			etc).
			Theoretical analysis and planning of experiments
			on directed modification of influenza virus he-
			magglutinin, interferons alpha and gamma.
			Theoretical results were successfully confirmed
			by experiments.

   1986-1990            Ph.D awarded in 1986.
			Development of the FCP method for prediction of
			functionally important regions in proteins.
			Development of databases on protein variable
			fragments and  biologically active proteins and
			peptides.
			Analysis of protein fragment variability.
			Investigation of some principles of protein en-
			gineering of vaccines.
			Theoretical analysis and planning of experiments
			on insertion of antigenic epitopes in M13 phage
			major coat protein (now known as "phage display"),
			HBsAg, HBcAg.
			Theoretical developments were used and confirmed
			by experiments.

    1990-present        Leading Researcher.
			Development of the method and computer program
			"Pro_Anal" for analysis of relationship between
			structure and activity in a family of proteins
			or peptides.
			Principal investigator of two projects on de novo
			design of fully artificial proteins - candidates
			for HIV-1 vaccine.
			Study of the structure-activity relations on
			disintegrins, interferons, tumor necrosis factor.
			Planning of experiments on enhancement of stabi-
			lity of erythropoietin and interferon-gamma.
			Analysis of peptide fragment insertions into ma-
			jor coat protein of phages M13, f1 and fd.

Grant  Support  in Russia:

"Protein engineering of vaccines. Design of a four-helix-bundle
protein as a candidate in anti-HIV vaccines".  1990-1993, Grant
N 28/92,  Academy  of  Sciences, Russia.

"A novel approach to vaccine design based on fully artificial
proteins with predetermined tertiary structure. Anti-HIV-1
vaccine development". 1991-1994,  Grant  N 12/92. AIDS project,
Academy of Medical Sciences, Russia

"Development of human recombinant erythropoietin with
prolonged activity". 1991-1994. Grant N 28-1/92,  Academy  of
Sciences, Russia.

Publications: Author of more than 60 publications.

		Selected publications:

1. A.M. Eroshkin , V.I. Fomin, P.A. Zhilkin, V.A. Kulichkov.
   Protein fragment variability analysis and some principles of
   protein engineering of vaccines.- Prot. Engineering, 1990,
   vol.3, p.425-431.
2. A.A. Iliychev, O.O. Minenkova, S.I. Tat'kov, N.N. Karpishev,
   A.M. Eroshkin, et al. M13 filamentous bacteriophage for
   protein engineering.- Molec. Biol. (USSR) 1990, vol.24,
   p.531-535.
3. A.M. Eroshkin. Amino acid similarity matrix for detection of
   antigenically similar sequences in unrelated proteins.-
   Molec. Biol. (USSR), 1988, vol.22, p.635-644.
4. A.M. Eroshkin, P.A. Zhilkin, I.K. Popkov, V.A. Kulichkov.
   Structural and physico-chemical properties of amino acids of
   functionally important regions of proteins.- Biophysics
   (USSR), 1987, vol.32, p.972-980.
5. A.A. Iliychev, O.O. Minenkova, G.P.Kshchenco, S.I. Tat'kov,
   N.N. Karpishev, A.M. Eroshkin, et al. Inserting foreign
   peptides into the major coat protein of bacteriophage M13.-
   FEBS Lett., 1992, vol.301, n.3, p.322-324.
6. Petrenko V.A., Tat'kov S.I., Eroshkin A.M., Boldyrev A.H.,
   Pozdhiakov P.I., Sivolobova G.F. Primary structures of mutant
   forms of human alpha-2 interferon.  Doklady AN USSR, 1988,
   v.14 n.6, p. 810-814.
7  V.A. Petrenko, S.M. Kiprijanov, L.N. Semenova, A.N. Boldyrev,
   P.I.Pozdniakov, G.F. Sivolobova, A.M. Eroshkin. Obtaining of
   hybrid hemagglutinin gene of H1-H3 subtype influenza virus by
   the localized mutagenesis methods.- Doklady AN USSR, 1988,
   v.299, p.1506-1509.
8. A.A. Iliychev, O.O. Minenkova, S.I. Tatkov, N.N. Karpyshev,
   A.M. Eroshkin, V.A. Petrenko, L.S. Sandakhshiev. Obtaining of
   viable  M13  bacteriophage with inserted foreign peptide in
   main coat protein.- Doklady AN USSR, 1989, v.307, p.481-483.
9. Petrenko V.A., Kiprijanov C.M., Semenova L.M., Boldyrev A.N.,
   Pozdhiakov P.I., Eroshkin A.M., Sivolobova G.F. Directional
   reconstruction of influenza virus hemagglutinin gene.  Molec.
   Biol. (USSR), 1990, vol.24, n. 5,  p.1230-1240.
10.Zhilkin P.A. and  Eroshkin A.M. Protein and peptide
   functionally  important regions search method from amino acid
   sequence.  Biopolymers and Cells (USSR), 1990, 6,36-42.
11.Eroshkin A. M., Zhilkin P.A., Shamin V.V.  Artificial
   protein-vaccines with predetermined tertiary structure - a
   novel object for protein engineering. Application to anti
   HIV-1 vaccine design. Protein Engineering, 1992, (in press).
12.Fomin V.I., Eroshkin A.M., Zhilkin P.A., Nikulin A.E.
   Protein variable fragments data base and its use in chimeric
   protein design planning. Submitted to CABIOS. Ms. No. CFAB137
13.Eroshkin A.M., Zhilkin P.A., Fomin V.I.  Algorithm   and
   computer  program  Pro_Anal  for  analysis  of relationship
   between structure and activity in  a  family of proteins or
   peptides. (in press) CABIOS, 1992.
14. Kiprijanov C.M., Eroshkin A.M., Pozdniakov S.G., Bondarenko
   O.D., Pljasunov I.B.,Dmitriev I.P., Sandachshiev  L.S. Gene
   of human erythropoietin: expression in mammalian cells and
   site-specific mutagenesis. - In " New directions in
   biotechnology". - V Conference of Russian Federation, 18-22
   May 1992, Pushino, p.84.
15.Eroshkin A. M., Zhilkin P. A., Shamin V.V. Fully artificial
   proteins with predetermined tertiary structure - a novel
   approach to vaccine design. Anti-HIV vaccine development.
   Abstract from VII International Conference on AIDS, Florence,
   16-21 June 1991, Abstract M.A.1319.
16.Eroshkin  A.M., Fomin V.I., Zhilkin P.A., V.A. Kulichkov.
   Working out the rules of vaccine engineering based on the
   variability analysis of peptidic fragments in closely related
   proteins. Molec. Biol. (USSR), 1990, vol.24, n. 3,  p.638-
   648.
17.Samukov V.V., Mazurkova N.A. Kalashnikov V.V, Ofitcerov V.I.,
   Seval'e A.F., Mizenko G.A., Eroshkin A.M. Antigenic
   properties of synthetic fragments from heavy chain of
   hemagglutinin of influenza virus. Molec. Genetic. Microbiol.
   Immunol. (USSR), 1988, 7, 35-38.
18.Eroshkin A.M., Karginova E.A., Lomakin A.C., Kravchenko V.V.,
   Mizenko G.A., Pokrovsky A.G., Zhilkin P. A., Shamin V.V.
   Protein engineering of vaccines. Design of four-helix-bundle
   protein as a candidate in anti-HIV-1 vaccine  - In " New
   directions in biotechnology". - V Conference of Russian
   Federation, 18-22 May 1992, Pushino, p.82.
19.Kiprijanov C.M., Eroshkin A.M., Bondarenko O.D., Dmitriev
   I.P., Pozdniakov S.G. Human erythropoietin gene : expression
   in stably transfected mammalian cells and site-specific
   mutagenesis.  - In "Structure and function of regulatory
   polypeptides", International Symp.,  June 26-30, 1992,
   Moscow, p.34.
20.Eroshkin A.M., Zhilkin P.A., Fomin V.I.  Method for analysis
   of relationship between structure and activity in  a  family
   of proteins or peptides.   Application to the search of
   activity modulating centres in disintegrins.  In: "Structure
   and function of regulatory polypeptides", International
   Symp.,  June 26-30, 1992, Moscow, p.17.
21.Eroshkin A., Karginova E.A., Kravchenko V.V., Mizenko G.A.,
   Yastrebova O.N.,  Zhilkin   P.A., Shamin V.V.
   Design of four-helix-bundle protein as a candidate in anti-
   HIV vaccine.     Abstract from VIII International Conference
   on AIDS, Amsterdam, 19-24 July 1992, p. A39, PoA 2221.
22.Eroshkin A., Karginova E.A., Lomakin A.S., Kravchenko V.V.,
   Mizenko G.A.,  Zhilkin   P.A., Shamin V.V.
   Design of four-helix-bundle protein as a candidate in anti-
   Human Immunodeficiency Virus (HIV-1) vaccine. Molec. Biol.
   (Russia), 1992, in press.
23.Eroshkin A.M., Minenkova O.O., Fomin V.A., Ivanisenko V.A.,
   Ilyichev A.A.  Analysis of peptide fragment insertions into
   major coat protein of bacteriophages M13, f1 and fd. Relation
   of protein structural characteristics and viability of mutant
   phages. Molec. Biol.  (Russia), 1992, in press.

---
Peter Zhilkin
IBD NRC					Tel:   +1-204-984-4540
435 Ellice Ave. Winnipeg		Fax:   +1-204-984-5472
Manitoba R3B 1Y6 Canada			Email: zhilkin@zeno.ibd.nrc.ca