From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: D.pseudoobscura Adh gene for alcohol dehydrogenase
Date: 28 Dec 92 21:28:02 GMT
Distribution: bionet
Checksum: 47595 7

LOCUS       DROADHGN74   3463 bp ds-DNA             INV       01-DEC-1992
DEFINITION  D.pseudoobscura Adh gene for alcohol dehydrogenase
ACCESSION   X64483
KEYWORDS    ADH gene; alcohol dehydrogenase.
SOURCE      Drosophila pseudoobscura DNA.
  ORGANISM  Drosophila pseudoobscura
            Eukaryota; Animalia; Metazoa; Arthropoda; Uniramia; Insecta;
            Pterygota; Neoptera; Holometabola; Diptera; Brachycera;
            Cyclorrhapha; Schizophora; Drosophiloidea; Drosophilidae.
REFERENCE   1  (bases 1 to 3463)
  AUTHORS   Schaeffer,S.W. and Miller,E.L.
  TITLE     Estimates of gene flow in Drosophila pseudoobscura determined from
            nucleotide sequence analysis of the alcohol dehydrogenase region
  JOURNAL   Genetics 132, 471-480 (1992)
  STANDARD  full automatic
COMMENT     *source: strain=PS279; *source: isolate=Kiabab National Forest,
            Arizona; *source: chromosome=IV section 88;
               From EMBL    entry DPADHGN74;  dated 16-OCT-1992.
FEATURES             Location/Qualifiers
     mRNA            join(40..119,915..1020,1085..1489,1552..1967)
                     /note="adult mRNA (Adh)"
     mRNA            join(882..1020,1085..1489,1552..1967)
                     /note="larval mRNA (Adh)"
     promoter        8..14
                     /note="adult promoter (Adh)"
     prim_transcript 40..1967
                     /note="adult primary transcript (Adh)"
     exon            40..119
                     /note="adult exon 1a"
     intron          120..914
                     /note="adult intron"
     prim_transcript 882..1967
                     /note="larval primary transcript (Adh)"
     exon            882..1020
                     /note="larval exon 1"
     exon            915..1020
                     /note="adult exon 1b"
     intron          1021..1084
                     /number=1
     exon            1085..1489
                     /number=2
     intron          1490..1551
                     /number=2
     exon            1552..1967
                     /number=3
     polyA_signal    1944..1949
     polyA_site      1967
     CDS             join(928..1020,1085..1489,1552..1818)
                     /EC_number="1.1.1.1"
                     /gene="Adh"
                     /product="alcoholdehydrogenase"
                     /codon_start=1
                     /translation="MSLTNKNVVFVAGLGGIGLDTSRELVKRNLKNLVILDRIDNPAA
                     IAELKAINPKVTITFYPYDVTVPVAETTKLLKTIFAQVKTIDVLINGAGILDDHQIER
                     TIAVNYTGLVNTTTAILDFWDKRKGGPGGIICNIGSVTGFNAIYQVPVYSGSKAAVVN
                     FTSSLAKLAPITGVTAYTVNPGITKTTLVHKFNSWLDVEPRVAEKLLEHPTQTSQQCA
                     ENFVKAIELNKNGAIWKLDLGTLEPITWTQHWDSGI"
BASE COUNT      979 a    784 c    790 g    910 t
ORIGIN      
        1 cgtagtgtat ttaaggcact gcacatgtcg aggaccacga ttattgtcta cagtgcagtc
       61 agtagttgtc aggtgcagca ggcggaccag catttcttta aatctactca atcgatcaag
      121 taagtaactg taatccaagt acagtcggcc catcccactc ggttctgccg taactcaaga
      181 aatttcttga tagcgcagtt ccagttgtaa aacgtcgttt aaaaaaacat ctcgttggag
      241 cttgggttga aaaacgttcg agttgggcgt agaaaatttc aaatataaaa tatcaaaaaa
      301 gagaaaatat cttcccaaag cagctgtcca ttttttgtta aagtgataga tcaaaaattc
      361 gtatgacgta tttgaaaagt gctctttatg caaataattt cctcaattct aattctacaa
      421 gaagcacttt tggctcttgt ccagaaaaaa caatttgttt acacttgtcg gatgataaca
      481 gaagcggacc gacggctaag taacataaca gtcgcctgtt aagtaatgcc ctcactctct
      541 gttagatggc cgcgcgtgtc gactccgata gaccagcaga cacgcagtag ttttcctcaa
      601 gaacctgaac tctaaacata gacataattt acgatacctt gagactcaaa taaaaataca
      661 agtatgagtg gtattctatt cctgattaga ctcgcacaca cacacgtacg agtattaaca
      721 taaatttgcc ggcaaataac caaaacaaaa cgaacgcaaa cgatccgaac acgtaatgcg
      781 agagataaga aacagaaaag ctcgacgtga aagaagcttc tggcggaagc tcttgccgaa
      841 ctccctataa atacgagaca gaaaaccaga ataaatataa taacaaactg ttgaattgtc
      901 ccatcgattc ccagatcaaa agacagaatg tctctcacca acaagaacgt cgttttcgtg
      961 gccggtctgg gcggcattgg cctggacacc agccgggagt tggtcaagcg taatctgaag
     1021 gtaagagaga acgaattcca tggaatctat ggaatcctaa atttcaaaaa ttcatttatt
     1081 ttagaacctg gtcatcctgg atcgcattga caatccggct gccattgccg aactgaaggc
     1141 aatcaatccc aaggtgacca tcaccttcta tccctacgat gtgactgtgc ccgtcgctga
     1201 gaccaccaag ctcctgaaga ccatctttgc ccaggtgaag acaatcgatg tcctgatcaa
     1261 cggtgctggc atcctggacg atcatcagat cgagcgcacc attgccgtta actacacggg
     1321 cctggtcaac accaccacag ccattctgga cttctgggac aaacgcaagg gcggcccagg
     1381 cggcatcatt tgcaacattg gctccgtcac cggtttcaat gccatctacc aggtgcccgt
     1441 ttactccggc tccaaggcgg cggtggttaa ctttacctcc tccctggcgg taagaaatcc
     1501 tacaactatc ccaaaatccc caaaactaaa tatttctaaa tattcccata gaaactggct
     1561 cccattactg gtgtcactgc ttacactgtc aatcctggca tcactaagac cactctggtc
     1621 cacaaattca actcgtggct ggatgtggag ccccgtgtgg cagagaagct gctcgagcat
     1681 cccacccaga cctctcagca gtgcgccgag aactttgtga aggccattga gctgaacaag
     1741 aacggtgcca tctggaagtt ggatttgggc accttggagc ccatcacatg gacccagcac
     1801 tgggactcgg gcatctaaac catcccagag actctatggg acatggcgtt ttagctttta
     1861 gtttcttttt ttccactcaa ttgttacgta tatacataca catatggcaa taaggctgat
     1921 ttgaaccccg ttttgaatat gataatacaa aattatattt gagaaatttc aacaaaatcg
     1981 ataagagcta tgctctcttt taattaaaaa aactagataa aaagagcaat ggtcagtggt
     2041 ggtcagtggt cgttgtggcc tctatcgatt tccacacaaa aactttactg tttagtaata
     2101 gaaaagaact tgagaaaggc agccaaaaga atgtacgatc tgacgggtaa gcatgtctgc
     2161 tatgtggctg actgcggtgg cattgcactg gagaccagca aggttctcat gaccaagaat
     2221 atagcggtga gtggtagagt ggtgtagaga gtggagagtg ttccgaagtg gttctggttc
     2281 ttaaacaagt ctctagtctc tagtctcttg gctgctgctg gtagctgtcg aaactaatta
     2341 agttaatgac tttttcatgt tttgtttttg tccgctgact tttgctgttc aaagctacaa
     2401 ttaagtttat gttttgagtt tgatctactt ttgcttaagg gaagtacttt cgattacgga
     2461 ttgctggcat ataaaaatag ataccaagga atgtgcatca tatcatattg attccatctt
     2521 gattctttgc ccagaaactg gccatccttc agagcgtgga aaaccccccg gccatcgctc
     2581 agttacaatc gattaagcac agcacacaga tcttcttctg gaccttcgat gtgaccatgg
     2641 cccgcgagga gatgaagaag tacttcgatg aggtcatggt ccagatggac tacatcgatg
     2701 tactgatcaa tggggccacc ctgtgcgatg agcggaacat tgatgccacc atcaatacga
     2761 atctgaccgg catgatgaac accgtggcca ctgtgctgcc ctatatggac cgtaagatgg
     2821 gcggttcggg tggactgatt gtgaatgtca cctccgtcat aggactggat ccatcgccag
     2881 tcttttgtgc gtacagtgcc tcaaagtttg gtgtgattgg gttcacccga agtctagcgg
     2941 tgagtgggcg agatcttaac ttgaaaatcc tctctaatgt cttgcattct ttcgcaggat
     3001 cccctgtatt acactcaaaa tggtgtggct gtcatggccg tctgctgtgg tcccaccaaa
     3061 gtgtttgtcg atcgggaact gactgccttc ctggaatacg gccagtcctt tgccgatcgc
     3121 ctgcgtcgtg ccccctgcca atcgacggcc gtctgcggcc agaatatagt aaatgccatc
     3181 gagagatcgg agaacggaca gatctggatt gccgacaagg gcggcctgga gtcggtggcc
     3241 ctccactggt attggcatat ggccgatcag tttgtgaact atatgcagag cactgacgat
     3301 gaggatcagg agtttttttt gggccagcga tgaggtggaa taaaacatca aatgtgtcac
     3361 aaaaagtagg caacttgttt gtgggtttgg aaatagattt gaaatatttt gaggtcatat
     3421 gaataatgac ataaatgagg gtgcctgaaa gtatgccaag ttt
//