From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Plasmid pOP2621 ORF1 gene, 5' end; BPO-A1 gene, complete cds.
Date: 29 Dec 92 19:59:00 GMT
Distribution: bionet
Checksum: 35068 4

LOCUS       P26BPO       1711 bp ds-DNA             BCT       10-DEC-1992
DEFINITION  Plasmid pOP2621 ORF1 gene, 5' end; BPO-A1 gene, complete cds.
ACCESSION   M84990
KEYWORDS    BPO-A2 gene; bromoperoxidase; halogenating activity.
SOURCE      Plasmid pOP2621 (library: ATCC10762) DNA.
  ORGANISM  Plasmid pOP2621
            Prokaryotae.
REFERENCE   1  (bases 1 to 1711)
  AUTHORS   Pelletier,I., Pfeifer,O., Altenbuchner,J. and Van Pee,K.-H.
  TITLE     Molecular cloning and sequencing of a non-haem bromoperoxidase gene
            from Streptomyces aureofaciens ATCC 10762
  JOURNAL   J. Gen. Microbiol. 138, 1123-1131 (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     CDS             complement(1..450)
                     /partial
                     /note="ORF1"
                     /codon_start=1
                     /translation="MTSQPHLHQHAAEIQEFGTVTQLPIALSHDARQYSCQRLNRVLA
                     DTQFLYALYKKCHWGMRGPTAYQLHLLFDKHAQEQLELVDALAERVQTLGGVAVGDPR
                     HAARITGVPRPPDGIEDVHSMLSRLLDAHETILADVRDAATRVAGLGD"
     CDS             706..1542
                     /gene="bpoA2"
                     /product="BPO-A2"
                     /codon_start=1
                     /translation="MPFITVGQENSTSIDLYYEDHGTGQPVVLIHGFPLSGHSWERQS
                     AALLDAGYRVITYDRRGFGQSSQPTTGYDYDTFAADLNTVLETLDLQDAVLVGFSMGT
                     GEVARYVSSYGTARIAKVAFLASLEPFLLKTDDNPDGAAPQEFFDGIVAAVKADRYAF
                     YTGFFNDFYNLDENLGTRISEEAVRNSWNTAASGGFFAAAAAPTTWYTDFRADIPRID
                     VPALILHGTGDRTLPIENTARVFHKALPSAEYVEVEGAPHGLLWTHAEEVNTALLAFL
                     AK"
BASE COUNT      257 a    649 c    560 g    245 t
ORIGIN      
        1 gtcgccgagc ccggccaccc gggtggcggc gtcgcggacg tcggcgagga tggtctcgtg
       61 cgcgtcgagg agccgcgaga gcatcgagtg cacgtcctcg atgccgtccg gcgggcgcgg
      121 caccccggtg atccgcgcgg cgtgccgggg gtcgcccacg gcgaccccgc cgagcgtctg
      181 cacccgttcg gcgagcgcgt cgaccagctc cagttgctcc tgggcgtgct tgtcgaagag
      241 caggtgcagc tggtatgcgg tcggcccgcg catgccccag tggcacttct tgtacagcgc
      301 gtacaggaac tgggtgtccg cgaggacgcg gttgagccgc tggcacgagt actgccgggc
      361 gtcgtgggag agcgcgatcg gcagctgggt gacggtgccg aactcctgga tctcggcggc
      421 gtgttgatga aggtgtggct ggctggtcat ggcgctcctg gggacatctg gatcgttcgg
      481 gggatcaggc taggaacgcg cgggcccacg gcctgttccg accgcgcact tcctgcggtc
      541 cttccgcgca cccgcgcggg agttgagccc gtgtgagccg ccgggcgcac gcggagcact
      601 ctcggacgac accgggtgct ctccgaggca acaagccggc gacggccgcc cctagcgtga
      661 gccccatcgc agccacacgc tgtccggaag ataggaagaa accccatgcc gttcatcacc
      721 gtcggccagg agaactccac ctccatcgac ctctactacg aggaccacgg cactgggcag
      781 ccggtcgtcc tcatccacgg gttcccgctg agcgggcact cctgggagcg gcagagcgcc
      841 gcgctgctcg acgccggcta ccgggtcatc acctacgacc gccgcggctt cggccagtcc
      901 tcccagccca ccaccggcta cgactacgac accttcgccg cggacctcaa caccgtgctg
      961 gagaccctcg acctccagga cgccgtgctg gtcggcttct cgatgggcac cggcgaggtc
     1021 gcccggtacg tgtcctccta cggcactgcc cgcatcgcca aggtcgcctt cctggcctcc
     1081 ctggagccgt tcctgctgaa gaccgacgac aacccggacg gcgccgcccc gcaggagttc
     1141 ttcgacggca tcgtcgccgc cgtgaaggcc gaccgctacg ccttctacac cggcttcttc
     1201 aacgacttct acaacctgga cgagaacctc ggcacccgga tcagcgagga ggccgtccgc
     1261 aacagctgga acaccgcggc gagcggcggc ttcttcgccg ccgcggccgc gcccaccacc
     1321 tggtacaccg acttccgcgc cgacatcccc cgcatcgacg tccccgcgct gatcctgcac
     1381 ggcaccggcg accgcaccct gcccatcgag aacaccgccc gggtcttcca caaggcgctg
     1441 ccctccgccg agtacgtgga ggtcgagggc gcgccgcacg gcctgctctg gacccacgcc
     1501 gaggaagtga acaccgcgct gctcgccttc ctcgcgaagt gacccggacg gccgcgttcc
     1561 cccgaccgcg gccgtccgtc ccatcgccgg taccgccgtc accgccccgg gtccggacgg
     1621 cgacggcggt accgaccaca cacccgcgcc cggagcccgc tcgagcgggc tccgggcgcg
     1681 aaccatgacc aggaggtcac ccgtgaagct t
//