From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: E.coli fimbrial adhesin subunit F1845 antigen gene, complete cds.
Date: 6 Nov 92 18:27:43 GMT
Distribution: bionet
Checksum: 61660 4

LOCUS       ECODAAEDA     884 bp ds-DNA             BCT       02-NOV-1992
DEFINITION  E.coli fimbrial adhesin subunit F1845 antigen gene, complete cds.
ACCESSION   M27725
KEYWORDS    antigen; fimbrial adhesin; fimbrial subunit F1845.
SOURCE      E.coli (diarrhea isolate C-1845) DNA, clone pDAS105.
  ORGANISM  Escherichia coli
            Prokaryotae; Gram-negative facultatively anaerobic rods;
            Enterobacteriaceae.
REFERENCE   1  (sites)
  AUTHORS   Bilge,S.S., Clausen,C.R., Lau,W. and Moseley,S.L.
  TITLE     Molecular characterization of a fimbrial adhesin, F1845, mediating
            diffuse adherence of diarrhea-associated Escherichia coli to HEp-2
            cells
  JOURNAL   J. Bacteriol. 171, 4281-4289 (1989)
  STANDARD  full automatic
REFERENCE   2  (bases 1 to 884)
  AUTHORS   Bilge,S.S., Apostol,J.M., Aldape,M.A. and Moseley,S.L.
  TITLE     Messenger RNA processing independent of RNase III and RNase E in
            the expression of the F1845 fimbrial adhesion of Escherichia coli
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. (1992) In press
  STANDARD  full automatic
COMMENT     Draft entry and computer-readable sequence for [1] kindly provided
            by S.L.Moseley, 08-SEP-1989.
FEATURES             Location/Qualifiers
     mRNA            72
                     /function="processing site"
                     /gene="daaE"
                     /evidence="EXPERIMENTAL"
     mRNA            73
                     /function="processing site"
                     /gene="daaE"
                     /evidence="EXPERIMENTAL"
     sig_peptide     310..372
                     /gene="daaE"
                     /codon_start=1
     mat_peptide     373..786
                     /product="fimbrial subunit F1845"
                     /gene="daaE"
                     /codon_start=1
     stem_loop       845..863
                     /gene="daaE"
                     /evidence="EXPERIMENTAL"
     CDS             310..789
                     /evidence="EXPERIMENTAL"
                     /gene="daaE"
                     /product="fimbrial subunit F1845"
                     /codon_start=1
                     /translation="MKKLAIMAAASMIFTVGSAQATFQASGTTGITTLTVTEECRVQV
                     GNVTATLARSKLKDDTAIGVIGVTALGCNGLQAALQADPDNYDATNLYMTSRNHDKLN
                     VKLKATDGSSWTYGNGVFYKTEGGNWGGHVGISVDGNQTDKPTGEYTLNLTGGYWTN"
BASE COUNT      251 a    224 c    248 g    161 t
ORIGIN      
        1 gaattacgtc atccgggaag cacacagatg acgcgcactg gtcaggcgca tcgtggtggc
       61 gaacaccggc tgaacacggg gccaccggac tggcagaccg tggaataagg catcaccgtg
      121 aacgttgtct gcggctttat gagcaagccc tgcagtcaga aacttactta tatgcaatga
      181 acagtctctg ctgcgggtgc agacatctgt gaacgttggt taatgtgggg taagacagct
      241 tactgattct gggatgaatt agaccgtact gttgtgttac cccctcacaa aactgaacag
      301 gtaatcaata tgaaaaaatt agcgataatg gccgccgcca gcatgatttt cactgtgggc
      361 tccgcgcaag ccacgttcca agcgagcggg acaacgggta ttaccacact gactgtgacc
      421 gaagagtgcc gggtgcaggt cggtaacgtt accgcgacgc tcgcgcgaag caagctaaaa
      481 gacgacacgg ctataggggt gatcggggta accgctctag gatgcaacgg cctgcaggcc
      541 gcgttgcagg ccgacccaga caactacgat gcgacaaatc tatatatgac tagccgcaac
      601 catgacaaac ttaatgtcaa actaaaagcc acagatgggt ccagttggac ctacgggaac
      661 ggcgtgttct acaaaaccga aggcggtaac tggggtgggc acgttgggat ctccgtggac
      721 gggaaccaaa ctgataaacc gaccggtgaa tacacactga acctcaccgg gggttactgg
      781 acgaactaag gccaaggatt cccatgacaa ctaaacgaaa ccgataacga acacaagagg
      841 tctaggggca ggtgcgctgc cccacgaaag atcatcgaaa acgc
//