From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Silene cucubalus glutathione-S-transferase gene, complete cds.
Date: 22 Dec 92 22:57:23 GMT
Distribution: bionet
Checksum: 61488 10

LOCUS       SIPGTSTFA    6230 bp ds-DNA             PLN       30-NOV-1992
DEFINITION  Silene cucubalus glutathione-S-transferase gene, complete cds.
ACCESSION   M84969
KEYWORDS    glutathione-S-transferase.
SOURCE      Silene cucubalus (library: lambda Charon 4A) cell suspension
            culture DNA.
  ORGANISM  Silene cucubalus
            Eukaryota; Plantae; Embryobionta; Magnoliophyta; Magnoliopsida;
            Caryophyllidae; Caryophyllales; Caryophyllaceae.
REFERENCE   1  (bases 1 to 6230)
  AUTHORS   Praendl,R. and Kutchan,T.M.
  TITLE     Nucleotide sequence of the gene for a glutathione-S-transferase
            from cell suspension cultures of Silene cucubalus
  JOURNAL   Plant Physiol. 99, 1729-1731 (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     exon            <2549..2695
                     /number=1
     TATA_signal     2467..2473
     intron          2696..4415
                     /number=1
     exon            4416..4464
                     /number=2
     intron          4465..4580
                     /number=2
     exon            4581..>5038
                     /number=3
     CDS             join(2549..2695,4416..4464,4581..5038)
                     /EC_number="2.5.1.18"
                     /product="glutathione-S-transferase"
                     /codon_start=1
                     /translation="MTIKVHGNPRSTATQRVLVALYEKHLEFEFVPIDMGAGGHKQPS
                     YLALNPFGQVPALEDGEIKLFESRAITKYLAYTHDHQNEGTSLIHKEKHEMAAQLVWE
                     EVEAHQFDPVASKLAWELVFKGIFGMQTDTTVVEENEAKLAKVLDVYEARLTESEYLG
                     ANDSFTLVDLHHLPLLGYLMGTQVKKLFEERAHVSAWCKKILARPSWEKTLALQKQA"
BASE COUNT     2015 a   1072 c   1140 g   2002 t      1 others
ORIGIN      
        1 gaattcacct gacggttgca caagattgga gaagccacaa aaagttatcc ttgtagatga
       61 gcctagcttt ttcccgcaac tgatctatag tctacaaaca gatgagataa tataaggttc
      121 atcccaaacc aaggaatcct tggagccaaa gaagtgagtt ttgcattttc tgtacaacca
      181 cctgaatgtt tgtaatcaaa ttgagtgtgg tgcaacattt attaagtggt tcctcagtta
      241 ctactgatgt aaaactgcta ttcagatttt caggaacaat cattttggaa taatacaaat
      301 ctaattcatg tgagttctta tagtatcaca gtacaaaatc gatcgaagaa ggtaaatgag
      361 acaaagcaaa caactgatac gtagttcaca agcacacgag ataccatatc agcaggttca
      421 cccaatccaa acccaaatga atcaaaatta gggtttcgcc ttgcccaaaa ttcagcagtt
      481 acttcaaatc acagtcaaat aataggacaa gataccatta atgtactaat aaataaaatc
      541 ggtaaccaaa atcaacaaga aagcaagcat ccaacaaaca aaaacaataa agagtaacag
      601 cggaaggacg ataaaaggga aaaaagggac ctgaagttat caatagggaa aggactgagt
      661 tgaacatcgt cgagacatca actttttttc gccatcgtcg ataggaaacg accctaaatc
      721 aattattgat aataataatt attagagttt gagtagaaaa ccttagatct gaacaaaggg
      781 gggaacctgt ggtgttaaga gtaacagcgg aaggaagata agaaacccgg tgaataatgg
      841 atgtgggtaa tgacgacgag gacgacggtg aaacatgggt taggttagag tgttggtggt
      901 ggcagagtag aacaacggtg aaagaggggt taggttagag tgttgagtga atgtgtgata
      961 gagaatgtga gatatatcgt atacgcaggc gttagtaatt ttaagccaat tttggagaaa
     1021 ttaagacatc agagtttaat atgtttgatg ttgtttaaag agcacgaaca atggacatca
     1081 gttttaagaa ttatccgatg tcaaaataat tatatacaca tcgggttttt tcagaacttt
     1141 gatgtctatg atttatacat cagttttttt aataatcaga tgtctataaa gtgatgtcta
     1201 ttacgatttt tgtagtagtg tgacctagta gacgagagcc tctaggaccc aatatatgga
     1261 cgatattatt atcgggaatg ctaatgttgt gaaatgggta atccttgtgc ctaagcaatt
     1321 gttgttctta ctatctaatc ttaataatgg catgacttta tatatgtgat ttgcatgtgt
     1381 gacccaatcc cctttgactc tctttattat attgtttaca tcgtattttc tagactatac
     1441 aaaccaaacc aaacccaaat cgataattcg accttgatag aaattcacca tagcaactca
     1501 tgcgtagtag acattttctc tgtggattcg accccttaat actacattaa tttgttgtgc
     1561 ctagggtatc tttgaatagg tgtgcgatag cctatcaggt aaactctttc tcctcggccc
     1621 gaaccaaact tagaagccca tgtatacact ccgtagtaaa cctggccatg ggacaagctg
     1681 gggttgattc gagccaacct gcgtcggggc tggccctaaa ccagcgtgag tcgcgtggca
     1741 aggcaaggat cgagtcggat ggggcttatt catgcttggc cgtaaatcag ctatcagcct
     1801 gtgctaccaa tcattttatt tatttattta tttattttag caggcttgga taggtcgggc
     1861 ctaacactcg agtctcgact cagcctggtt cagggctggt taagcccagc ttttggccgg
     1921 ccagacgagc tttggaatcg acaaacccgc aagaagggtt ggtttaggcc ccggttgaca
     1981 attatttggt ctcgcctatt ccatagtata tactctcttt gcctgtcatt tgtttgcctt
     2041 taattttaac acaaagacca aggaaatggg aggggtcaat tattagatga caagttgacc
     2101 aaattaagtg taaatgctcc atttatccat cgaaaatatt tctaaaatga aaagacaaac
     2161 aattaactga aaaaacctaa aattaaacaa acgaccggga cgaagtgagt aattaagtac
     2221 atagtataac ttacaaatca agaaattttc tccaatcaac gtcagagtat acgtatccac
     2281 gtttcgaaga ccaatcaaga tgcaacttct taacaagttc cactccatta aaaagtcaac
     2341 taacattaaa aattagtcgt ccatagtaca tgctaaaaca actcattcca ggaaacgaat
     2401 atactataca ataatggtgg gatttaatta taatattgtc acaaaactat atactaattc
     2461 aacatctata aattccaccc tcctagaata cgtgattatc tcatttcatt acataataat
     2521 tcaaaacaaa taatagcaaa taataataat gacgatcaag gtgcatggaa acccaaggtc
     2581 caccgcgact caacgtgtct tggttgcgct ttatgagaaa caccttgaat ttgagtttgt
     2641 acccattgac atgggtgctg gtggtcacaa acaaccgtct tacctcgccc ttaacgtatg
     2701 ttcgtgtctt tttttttaaa attcatattt tgttatttat gagtagaggt atctataagt
     2761 caaccattta taagtcaagt tccgactttc tatcttagat agattagcat tataaatcaa
     2821 aatgataatt gaaatcacga ctttcttagg tatctataag tcaaccattt ataagtttga
     2881 tctgaatgga gtacacaact gttaatctat ctctttgatc tacataaaca ttttattttt
     2941 gtatttatac tagatttaag tagtgtatga aattttttac catggaactg acccgggcaa
     3001 ccagtagacc agcttgatta ttattctaac gatcttactc ttagtgctag aaaagaggta
     3061 gatttgcgaa caatctaaaa tgaaaattgt gacgagaact acaccaaata atttgacttt
     3121 tagagtaaaa acaactgcca ttaatttagt aagtcatttt gctttatatt atcataattc
     3181 agatcaaaga ctagcgaatc tttcgtcatt atttgaaatg aaacttgaaa aaacgagaaa
     3241 aaaaaatata ttactaaatc taatgacttt taggttttaa tacagcatcg aatacgtaat
     3301 ctgtttaaga aattgagggc tactatgttt ttagagattt aatcatgttc ccatctatat
     3361 ggcaatatgg atatatcatt ttctctttaa attatttggg atatttactt agtacctcta
     3421 tgttttttct tttttctgtt ttataaggtt catccccttt tttgcactaa aaaaccttgg
     3481 tggactacaa ctcatggtca cgaatttaaa aagtaaccat ttcttttcta aattaatcgt
     3541 cttttttgag atcttcgatt ttaaaaagaa aaataaatct ccgttttatg aactcatcgc
     3601 aagaatcacg gtcgatcaac tttgtaacat gaaaacgtgg aatagaagga tacgttagat
     3661 ttagattgaa tataataata tgggtaaggc ccaagagccc atgttggaaa ccagtaggtc
     3721 ttgcttgtga cgggtcggat cttacgacgg gtattttgtg agaggaaata ggtagatgag
     3781 acaaggtggg caccccccat gtgcttctcc actcaccctt tatgagtatt ttgtgagaga
     3841 aaatggtatc cgtcactcat ttgtgacgga tagtgtccgt cacaaatgag aatttgtggt
     3901 tggaaacaga gtatgtggat ctggcattga ttcttcaatt agtgcttatg acatggtccc
     3961 atattaactt tggagttgta caatgcaaga acaatcaagt taattaaaca actctaccca
     4021 tatacgcatt atattttaat caaagaacaa ttatggcatt ttcatttcat gtgaatttgg
     4081 cctcatcaac atttatgata aattgataag tgtagcgcaa aagtgttaat taagtccttt
     4141 aactttgttc accgatcaat aattatctat ggttatactt tgtgaaggta attattttca
     4201 ccaagaaaaa ataatttgtg aagacaatga ataaatattg actgggacga ataagtaatt
     4261 gctaaagtta ataggagtac taagcactaa gtgtagatta atttcataat ccgtattaga
     4321 taagtcaatt tcggaactta taagggttgt aagcaagact taatctctga agatagatga
     4381 acttataatt tgttccttct aatgtatgaa tacagccatt tggtcaagtg cctgctcttg
     4441 aggatggaga aatcaagctt tttggtaatt ttcccttaac tcattcatta ttcctattta
     4501 gtcacacttt gtttattttc atattttaaa aaaaaataga aggaaattac caatttctaa
     4561 caaatggcat tctcaaacag agtcaagagc tataacaaag tacttagcat acacacacga
     4621 tcaccaaaac gagggaacct cattgattca caaggaaaaa cacgaaatgg cggcccagtt
     4681 agtctgggag gaggtagaag ctcatcagtt tgacccagtg gcgagtaaac ttgcatggga
     4741 gcttgttttc aagggtatat ttggaatgca gactgatacg actgtggttg aggagaatga
     4801 ggctaagtta gctaaggtct tggatgtcta tgaggcgcga ttgaccgagt ccgagtactt
     4861 gggtgccaat gactccttca cattagttga tcttcatcac ttgccactct tgggttactt
     4921 aatggggact caagtgaaga agttgtttga ggagagggct catgtgagtg catggtgtaa
     4981 gaaaatcttg gctaggcctt cttgggagaa gaccttggct cttcaaaagc aagcttaatt
     5041 aattatgatg aaatatttga ttagctttca tttaagtcaa aaatgaaatt aatatttcca
     5101 atttctagtt tgttttgatt gttgtattgt gttgttgctt gcctacttta ccttggtgta
     5161 ttttgttcaa gttgtgttta tcatgcgaat aataatactg gatcagagtt ctttgcgttt
     5221 ttttactttg aatttatgtt atttaatcat gtgatatatg tataactgta tgtttgtcta
     5281 atggaccata tataatactc cctcccatcc tctattttct tccctatttc ctaaaacgga
     5341 ttattcaggt tttcttcccc tttctatatt tggtaacttt tactcttatt ttattcatcc
     5401 ctctctccta tcacacaacc ccactcaact cttttacttc tattttattc cttcctttac
     5461 tatttgtggc ccacaattta ttatttaact cctaattatt cattcctctc tcctattccc
     5521 aaacccaccc aactttttat caatattata ctccttccct taatctttgt gcccaaaaga
     5581 aaagggaaga aaatagagga tgggagggag tagtcatgtt atcaaccttt attaaatcag
     5641 ttgatgacgt gtttcctttg tgtttacact atccggattt tatctagatt cgagaagagt
     5701 aggaccatca aaacggtaaa acaagtgtag catatacgac tgccatttat aattatgtcg
     5761 ttaaagatta gattagtaaa tgcttctaat aagtatgatg acaaagatta gattagtaaa
     5821 tgcttctaat aagtatgatg acaaggccga cagaaataat tatgcaaatg tactccctat
     5881 attcaatcaa ttattgtttc gataattgtc aaattaacta tcatcataca taattaaaag
     5941 aaatttcgac gacgccacct actcataaga attaaagtca aactcatgag tcagagtcat
     6001 gacatgcagg gccgncccta tagggtgttt aggttggcaa ttgacaaggg cccaagtcta
     6061 gaaggggccc attagtaaga aattagagtc aaataaaatt tattacatgt aaaattcata
     6121 ttaaaggaag tagtctagtg gttttctatc ttgctaagtc ataagagatc ttgtgtttta
     6181 ttcctcatgg cttcttttca ccatttctac tttttttttt tttggaattc
//