From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Bacillus cereus neutral protease (NPRC) gene, complete cds.
Date: 24 Jul 92 10:05:13 GMT
Distribution: bionet
Checksum: 57872     5

LOCUS       BACNEUTPRO   2039 bp ds-DNA             BCT       24-JUL-1992
DEFINITION  Bacillus cereus neutral protease (NPRC) gene, complete cds.
ACCESSION   M83910
KEYWORDS    NPRC gene; extracellular proteolysis; neutral protease.
SOURCE      Bacillus cereus (library: DSM 3101) DNA.
  ORGANISM  Bacillus cereus
            Prokaryotae; Firmicutes; Endospore-forming rods and cocci;
            Bacillaceae.
REFERENCE   1  (bases 1 to 2039)
  AUTHORS   Wetmore,D.R., Wong,S.-L. and Roche,R.S.
  TITLE     The role of the pro-sequence in the production and secretion of the
            thermolysin-like neutral protease from Bacillus cereus
  JOURNAL   Mol. Microbiol. 6, 1593-1604 (1991)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     sig_peptide     217..297
                     /gene="NPRC"
                     /codon_start=1
     CDS             217..1917
                     /product="neutral protease"
                     /gene="NPRC"
                     /note="putative"
                     /codon_start=1
                     /translation="MKKKSLALVLATGMAVTTFGGTGSAFADSKNVLSTKKYNETVQS
                     PEFISGDLTEATGKKAESVVFDYLNAAKGDYKLGEKSAQDSFKVKQVKKDAVTDSTVV
                     RMQQVYEGVPVWGSTQVAHVSKDGSLKVLSGTVAPDLDKKEKLKNKNKIEGAKAIEIA
                     QQDLGVTPKYEVEPKADLYVYQNGEETTYAYVVNLNFLDPSPGNYYYFIEADSGKVLN
                     KFNTIDHVTNDDKSPVKQEAPKQDAKAVVKPVTGTNKVGTGKGVLGDTKSLNTTLSGS
                     SYYLQDNTRGATIFTYDAKNRSTLPGTLWADADNVFNAAYDAAAVDAHYYAGKTYDYY
                     KATFNRNSINDAGAPLKSTVHYGSNYNNAFWNGSQMVYGDGDGVTFTSLSGGIDVIGH
                     ELTHAVTENSSNLIYQNESGALNEAISDIFGTLVEFYDNRNPDWEIGEDIYTPGKAGD
                     ALRSMSDPTKYGDPDHYSKRYTGSSDNGGVHTNSGIINKQAYLLANGGTHYGVTVTGI
                     GKDKLGAIYYRANTQYFTQSTTFSQARAGAVQAAADLYGANSAEVAAVKQSFSAVGVN
                     "
     mat_peptide     964..1914
                     /function="extracellular proteolysis"
                     /standard_name="Bacillus cereus neutral protease"
                     /product="neutral protease"
                     /gene="NPRC"
                     /note="putative"
                     /codon_start=1
BASE COUNT      708 a    325 c    433 g    573 t
ORIGIN
        1 ctgatattgt tggtcaacaa gctaaaactg ttgaacaagt taatttataa tctccccccc
       61 tttccaaccg gaaaggggtt tttcaatatt tgttcctcaa aattctacaa aacttgagaa
      121 aaaaattatt tgaattttta gtatattaat agtggaaaca taatgctaat atgaaacact
      181 ctttttcaaa aaaatttttt attaggggga aggttcatga aaaagaaaag tttagcgtta
      241 gtgttagcga caggaatggc agttacaacg tttggaggga caggctctgc atttgcggat
      301 tctaaaaatg tgctctctac gaagaagtac aatgagacag tacagtcacc tgagtttatt
      361 tctggtgatc taactgaagc aactggcaag aaagcagaat ctgttgtgtt tgattactta
      421 aacgcagcaa aaggtgatta caagctaggg gaaaagagtg cacaagattc tttcaaagtg
      481 aaacaagtga agaaagatgc tgtaactgat tcaacagtag tacgtatgca acaagtttac
      541 gaaggagtgc ctgtatgggg ttctactcaa gtagctcacg taagtaaaga cggttcttta
      601 aaagtattgt ctggaacagt tgcacctgat ttagacaaaa aggaaaagtt gaaaaataaa
      661 aataagattg aaggcgcaaa agcaattgaa atcgcgcagc aagatttagg ggtaacaccg
      721 aaatatgaag tagaaccaaa agcggactta tatgtatatc aaaacggtga ggaaacaaca
      781 tatgcatatg ttgtaaatct aaacttctta gatccaagcc caggaaacta ctactatttc
      841 attgaggcag acagcggtaa agtattaaat aagtttaata caattgatca tgtgacgaat
      901 gatgataagt caccagttaa gcaagaggct cctaaacagg atgcgaaagc tgttgtaaag
      961 cctgtaacag gaacgaataa agtaggaact ggtaaaggtg tattaggaga tacgaagtcg
     1021 cttaatacaa cgttatctgg atcatcttac tacttacaag ataatacacg cggggcaacg
     1081 attttcacat atgatgcgaa aaaccgttca acattaccag gaacactatg ggcagatgca
     1141 gataatgttt tcaatgcagc gtatgatgca gcagcagtag atgctcatta ctatgcaggt
     1201 aaaacatatg attactataa agctacgttt aatagaaatt ctatcaatga tgcaggagcg
     1261 ccgttaaaat cgacagttca ttacggaagt aattataaca atgcattctg gaacggatca
     1321 cagatggtat acggagatgg tgatggtgta acgtttactt cattatctgg tggaattgat
     1381 gtaattggtc acgagttaac gcatgctgtt acggaaaata gttcaaatct aatttatcaa
     1441 aatgaatcag gggctttaaa tgaagcgatt tctgatattt ttggtacttt agtagaattc
     1501 tatgataacc gtaacccgga ttgggagatt ggtgaagata tttacacacc tggtaaagca
     1561 ggagacgcac ttcgctctat gagtgatcct acgaagtatg gtgatccaga ccattattct
     1621 aagcgttaca ctggttcaag tgataacggt ggtgttcata caaacagcgg cattattaat
     1681 aaacaagctt atttattagc aaatggtggt acgcattatg gtgtaactgt aactggtatc
     1741 gggaaagata aattaggtgc gatttactac cgtgcaaata cacagtattt cacgcaatct
     1801 actacattta gtcaagctcg tgctggtgca gtacaagctg ctgcagactt atatggtgca
     1861 aattctgctg aagtagcagc agttaagcaa tcatttagtg ctgttggtgt aaactaaggc
     1921 tttaaggaag agctagtaat aaaatacctc aaaaataaag aaggagcata ggctccttct
     1981 ttattttttt ctccattttt tccacaaata aaacaatcca ataacgcctg cgattgtta
//