From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: F.trinervia ppcA1 gene for phosphoenolpyruvate carboxylase
Date: 11 Sep 92 10:03:46 GMT
Distribution: bionet
Checksum: 11796    16

LOCUS       FTRPPCB      9012 bp ds-DNA             PLN       11-SEP-1992
DEFINITION  F.trinervia ppcA1 gene for phosphoenolpyruvate carboxylase
ACCESSION   X64143
KEYWORDS    phosphoenolpyruvate carboxylase; ppcA1 gene.
SOURCE      Flaveria trinervia DNA.
  ORGANISM  Flaveria trinervia
            Eukaryota; Plantae; Embryobionta; Magnoliophyta; Magnoliopsida;
            Asteridae; Asterales; Asteraceae.
REFERENCE   1  (bases 1 to 9012)
  AUTHORS   Hermans,J. and Westhoff,P.
  TITLE     Homologous genes for the C4 isoform of phosphoenolpyruvate
            carboxylase in a C3-and a C4-Flaveria species
  JOURNAL   Mol. Gen. Genet. 234, 275-284 (1992)
  STANDARD  full automatic
COMMENT     *source: clone_library=lambda DASH EcoRI; *source: clone=lnFtppc8;
            From EMBL    entry FTPPCA;  dated 03-SEP-1992.
FEATURES             Location/Qualifiers
     CDS             join(2185..2352,3185..3576,3654..3738,3830..4056,
                     4145..4247,4326..4413,4496..4650,5390..6388,7005..7391,
                     7996..8295)
                     /EC_number="4.1.1.31"
                     /product="phosphoenolpyruvate carboxylase"
                     /gene="ppcA1"
                     /codon_start=1
                     /translation="MANRNVEKLASIDAQLRLLVPGKVSEDDKLVEYDALLLDKFLDI
                     LQDLHGEDLKEAVQQCYELSAEYEGKHDPKKLDELGSLLTSLDPGDSIVIAKAFSHML
                     NLANLAEEVQIAYRRRIKLKSGDFADEANATTESDIEETFKRLVHKLNKSPEEVFDAL
                     KNQTVELVLTAHPTQSIRRSLLQKHGRIRNCLAQLYAKDITPDDKQELDEALHREIQA
                     AFRTDEIRRTPPTPQDEMRAGMSYFHETIWKGVPKFLRRVDTALKNIGINERFPYNAP
                     LIQFSSWMGGDRDGKHPRVTPEVTRDVCLLARMMTSNMYFSQIEDLMIEMSMWRCNSE
                     LRVRAEELYRTARKDVKHYIEFWKQIPPNQPYRVILGDVRDKLYNTRERSRHLLVDGK
                     SDIPDEAVYTNVEQLLEPLELCYRSLCDSGDHVIADGSLLDFLRQVSTFGLSLVKLDI
                     RQESDRHTEVLDAITQHLGIGSYREWSEEKRQEWLLAELSGKRPLIGPDLPKTEEVKD
                     CLDTFNVLAELPSDCFGAYIISMATSTSDVLAVELLQREYHIKHPLRVVPLFEKLADL
                     EAAPAAMTRLFSMDWYRNRIDGKQEVMIGYSDSGKDAGRFSAAWQLYKTQEQIVKIAK
                     EFGVKLVIFHGRGGTVGRGGGPTHLALLSQPPDTINGSLRVTVQGEVIEQSFGEEHLC
                     FRTLQRFCAATLEHGMNPPISPRPEWRELMDQMAVVATEEYRSVVFKEPRFVEYFRLA
                     TPELEFGRMNIGSRPSKRKPSGGIESLRAIPWIFSWTQTRFHLPVWLGFGAAFKHAIQ
                     KDSKNLQMLQEMYKTWPFFRVTIDLVEMVFAKGNPGIAALNDKLLVSEDLRPFGESLR
                     ANYEETKNYLLKIAGHKDLLEGDPYLKQGIRLRDPYITTLNVCQAYTLKRIRDPNYHV
                     TLRPHISKEYAAEPSKPADELIHLNPTSEYAPGLEDTLILTMKGIAAGMQNTG"
     CAAT_signal     2041..2045
     TATA_signal     2077..2083
     exon            2119..2352
                     /number=1
     prim_transcript 2119..8496
                     /gene="ppcA1"
     intron          2353..3184
                     /number=1
     exon            3185..3576
                     /number=2
     intron          3577..3653
                     /number=2
     exon            3654..3738
                     /number=3
     intron          3739..3829
                     /number=3
     exon            3830..4056
                     /number=4
     intron          4057..4144
                     /number=4
     exon            4145..4247
                     /number=5
     intron          4248..4325
                     /number=5
     exon            4326..4413
                     /number=6
     intron          4414..4495
                     /number=6
     exon            4496..4650
                     /number=7
     intron          4651..5389
                     /number=7
     exon            5390..6388
                     /number=8
     intron          6389..7004
                     /number=8
     exon            7005..7391
                     /number=9
     intron          7392..7995
                     /number=9
     exon            7996..8496
                     /number=10
     polyA_signal    8453..8458
     polyA_site      8496
                     /note="by similarity to cDNA clone pcFtppc5.5"
BASE COUNT     2817 a   1532 c   1597 g   3064 t      2 others
ORIGIN
        1 aagcttatgt ttgttggtag tttttctttt tgcattgtat atgatatcta gaacatgaaa
       61 aaaggactca ccaggacagg agtattgcat ctatgttttt attcgaatat ttctcgttac
      121 acaatagaaa aacaaaacaa atccacggaa aggataatga gcttatacgt ggacaatatt
      181 gagactatat ttctatggtt gaaatcatgt gaatttatga gagctgtact tactcactaa
      241 aacaaacaaa aaactaaaac aaacaaaaaa ctgaaacaaa caaaatcctc cataaaaaga
      301 tgaatcgaac attttctttt tgtcatgata catatatata tatatatata tatatatata
      361 tgtatatgta tatatatata tatatgtata tatatatata cacacacaca cacacatata
      421 tatatatata tatatatatg tatgtatgta tatatgtata tatatatgtg tgtgtgtgtg
      481 tgatatgttg catatatatt gagaaatgga aattaagtac aataatatct ctacatagag
      541 gcgttaatgc gtcaattttg tgtgttaaag acataatcta atacaacatt gaagtttttc
      601 tatgaatttg gaaagtatgt atacacaaaa attatgtaag agataaaaga cttgtgtgga
      661 aatcaaatac ctataaaata attgcaattg ttacgaaaga tgatttgttt atttatgtat
      721 taacttttta cataaaatac ctactaagtt tgatttttaa ataaaattac tcttaaattt
      781 tagcagaata accaggtaaa ctcataaaca catggtactg actttacact cacccattag
      841 ctaagtgtgt ttgtgataca accctatttc gtattatgta taaaactcac aaatctagtt
      901 gtatgcatta ggtgtgagat agccccagaa agttttatga gagggttgta tgtccaaaaa
      961 ctttatgatc atataggttt tgtttttttg tttttttgtt tttttgtttt tttgtttttt
     1021 tttgtttttt ttgttttttt gttttgtttt tttttgtttt gttttttttg ttttttttgt
     1081 tttttttgtt tttttgtttt tgtttttgcg tgtgcgtgta tgagagctag tgagggtacc
     1141 accgataaaa atgccactga aatcgatatt gaatataacg acctttgcaa gaagagaatt
     1201 aagtatttat gagtacatac ttttgaaggc tttctcgtat ttaatctttc gcaggtttaa
     1261 aaatattaat tatatattac accccaatgc ttttaaatct ttacaactta atccttataa
     1321 tttttcattt tcaactttga tcccatatac ttttttatat tttataaaat ttttatctta
     1381 cttttcagtc taaattttac gagttaacaa gcggcaacgt gcgcgtgtgg cttcaatgtt
     1441 tctacgcata tttttccatt tgacggcccc gtcacaacgc acaagtcata gatagaccta
     1501 gctattattt tttttaataa tattttacgt ttgtcatggg tgattcaacg tttttatgca
     1561 taattttcat gttgatttat ttatttttgt tgtactttat aatgcgagta tttccggtgt
     1621 taatgatgga tgatgttaaa tgacatcgtt ttaatactaa ttgtttttta atttacaaaa
     1681 ctctcaacaa atgattagtt gggttagtta ttcataggaa agcggacgag catgtcgtta
     1741 taattaaaaa aatatcaaaa gagtaaacaa aaaaggaaaa agactaatta tttagataat
     1801 aataatatcc acaaaaatat tcgaattctt caatcctgag tttgctctgt ggatgagttt
     1861 ctgtatcatt gatacttgat acctgtaatt cacacacctc atatctcata cttcatctat
     1921 aaatacccaa ttcattttgc tcaaagtctc aacactgagc atacccaata ttcaggtgat
     1981 ctaatttaac gtttgcatga gtattttctt aataaaattt atgttgggtt tacagtatct
     2041 attgggtgga tttcttaaac ggattgtggt ttgattaata aaaaatctta atgagaagtt
     2101 tgtgataata tgctgaaatg ggttgttttt gtgttaattt ttcagggttg gaggggaatt
     2161 aagtattaag caagggtgtg agtaatggct aaccggaatg tggagaaatt agcatcgatc
     2221 gacgctcagt tgaggctttt agtccctggg aaagtttctg aggatgataa gcttgttgag
     2281 tatgatgctt tgcttttgga taagtttctg gatattcttc aggatttgca tggggaagat
     2341 ctcaaggaag cggtatgata actcaaatta ttgtttgttt tttagcttac actctaatct
     2401 tgatgctcct tttgtttaat ccattacata agttagatct caaatgaatt aagatatgat
     2461 tatcactttt aattagagtt gggttttatg ggatttatac aactggtgaa tgtgaatctt
     2521 tagtttatta tcttatttgg ttttgcggga ctctttgttt taattgatca ccaactgttt
     2581 gacataatgc atcaaaggat ttcttcgctt ctttcttcgt ttgtttacta gatcaatctc
     2641 aagaaacaat tttatgtgat actttacata aaatatgaca caattttatg tgctacttta
     2701 ctaatcttga aaccaacgat tagagagatt ttaaagaaaa cataatgcta ctcctactta
     2761 taatgttaaa acaaaaaaaa acacatgcta aattaatcta ccaaaaccca ccatctccac
     2821 tcttagtcat catgtttccc acatatcctc catattactc ggtcaaacta ttgactaaat
     2881 cacattaatg tgtagacaat ttctgaccca ttcaaatgac ccatgaacac tttgacccgt
     2941 gaagactttg acccgtgacc catgacccat gaaccaaagc gattaatcca acaataactc
     3001 tggtagtcta gcagtatcaa catgtatact atagtggtcg ttcctaagtg gttgagagta
     3061 ctagtctcat aacggacaat ggtgtatatt tagagtaggt ttaaatggtg tgtgggttag
     3121 ctgttataaa atatatatac atgcacatag atgtataagt tagtaagtat gtctgcttat
     3181 gtaggttcaa caatgctatg agctatctgc tgaatatgaa ggaaaacatg acccgaagaa
     3241 gctggatgag cttggaagtc tgttgacaag tttagatcca ggggattcca ttgtcattgc
     3301 aaaagccttt tctcacatgc ttaacttagc caatctggct gaagaagttc agattgctta
     3361 ccgccgaaga atcaaactaa agagtggtga ttttgctgat gaggctaatg caacaactga
     3421 atcagatatt gaagaaactt tcaagagact tgtgcataag cttaacaagt cccctgaaga
     3481 ggtttttgat gcactcaaga atcaaactgt tgagttggtc ttgactgctc atccaactca
     3541 atccatccgc agatctttgc ttcaaaagca tggaaggtat acccttcaac cctatacatg
     3601 cttccaattt gaccgatttc tcaactttac aattgaccct cttatctttg taggattcgt
     3661 aactgtctgg cccagttgta tgccaaagac atcactcctg atgataagca ggaactggat
     3721 gaggctttgc atagagaagt gagtattaat gattagcaat cgagttagag tgaagatgca
     3781 ttgtgtgtgc aagatcattg ttattaattg ctttttatgt aattaataga ttcaagctgc
     3841 attccgtact gatgaaatca ggaggactcc accaacacca caagatgaaa tgagagcagg
     3901 aatgagttac ttccatgaaa ctatctggaa gggtgttcca aaattcttac gtcgtgttga
     3961 cactgcccta aagaatattg ggattaatga acgttttccc tataatgcac ctctaattca
     4021 attctcttca tggatgggtg gtgaccgtga tggcaagttt aagctatttt atacactaaa
     4081 ttgacaaaaa aactaagatt tcattctatc acttatataa atttgcttct ggattaaaat
     4141 gcaggcatcc gagggttact cctgaggtaa caagggatgt ttgtttgcta gccagaatga
     4201 tgacgtcaaa catgtacttt tctcagatag aggatcttat gattgaggta tgtgaaaaaa
     4261 actttcttgg atatatgtac tcttacatag tgattattga tctaaaacta tgttttcttt
     4321 tttagatgtc catgtggcgt tgtaatagtg aattacgtgt tcgagcagaa gaactgtaca
     4381 gaacagcaag aaaagatgtg aagcactaca taggtatttg ttagtgttaa taatgttatg
     4441 tattactaca tataaaaaac acagaataac gttatatgtt ggactttttc atcagagttt
     4501 tggaaacaga ttcctcccaa tcaaccttat cgtgtaattc ttggtgatgt aagggacaaa
     4561 ttatataata cacgtgaacg atctcgtcat ttattggtcg atgggaaatc tgacatccca
     4621 gacgaagctg tttataccaa tgttgaacag gtatcaaccc cttcaagatc accatttatt
     4681 aatttttgat aaatcctaga tttcattttt gacctggtta tgatcaggtc aatttgggct
     4741 atcttttatc tttaatgggt caaactagta aagtaaacaa attagctata aaggaaacaa
     4801 gtcaaaggtt gcgctagatt ttcttggata aatatgcata taacctcctg attcttatta
     4861 ttaaatagta tattttgatt tttttattga aatgtgtaaa acttatggaa aatgaccaca
     4921 ttcgaccagc atcaaaaact acacattttg accagctacc taaacctcga ggcctgcgca
     4981 ttttacccat ctcttatcct taccttcgta atgaggactt ctcactagac cctacacttt
     5041 aatgaatagg tcaacttggg ctatgtttta tcactaacat gtcaaatggg ttaaataaaa
     5101 tagtatagct aaaacaaaaa tgtggtcaaa tgggcgaaaa tctcccaaaa tgtattcatt
     5161 ataaaacctc ttgatgaatg tatgaaaagt gtatattgat tgaaaaagta taaagtttgt
     5221 aatcacattt gacgtaccga tagttttaaa gactgtaaaa cctttggatg acaatcagat
     5281 tcaacaggca cataaaatta cccattttga cctgttatcc caatgccttg cctctaatga
     5341 aaattttgct ttatgctctc tctaatatct ctcttataat atttcacagc tcttggaacc
     5401 actggagcta tgctatagat cactatgtga ctctggtgac catgtgattg ctgatggaag
     5461 ccttcttgat tttctaagac aagtgtcgac ttttggactc tcacttgtaa aacttgatat
     5521 aagacaagaa tctgaccgtc acactgaagt ccttgatgca atcactcaac atttaggaat
     5581 tgggtcctat cgtgagtggt ctgaagaaaa acgccaagaa tggcttctag ctgaactcag
     5641 tggaaaacgt cctcttattg gtccagacct tccaaaaact gaggaagtta aggattgttt
     5701 agacacgttt aatgttttag cagaactccc atctgactgt tttggtgctt acatcatctc
     5761 aatggccaca tcaacttccg atgtccttgc tgttgagctt ctccagcgcg aataccatat
     5821 aaaacatccg ttacgcgtgg tccccttatt tgaaaaactt gctgacctgg aggcggcccc
     5881 tgcggccatg acccgccttt tctcaatgga ttggtacaga aaccggattg acggtaaaca
     5941 agaagtcatg attgggtact ctgattcagg aaaagatgca ggccggttct ctgctgcatg
     6001 gcagctctac aaaactcaag aacagattgt taaaattgca aaagagtttg gagtcaaact
     6061 tgttatattt catgggcgtg gtggaactgt tggtagaggt ggtgggccca cacatctggc
     6121 tcttctctct caaccaccag acaccattaa cgggtcttta agagtcacgg ttcagggtga
     6181 ggtcatagag cagtcgtttg gtgaggaaca tttgtgcttt agaacacttc aaagattttg
     6241 tgcagctaca cttgagcatg ggatgaaccc accaatctca ccacggcccg agtggcgtga
     6301 acttatggac cagatggctg ttgttgcaac cgaggagtac cgttctgttg tgttcaagga
     6361 accgcgtttt gtggagtatt tccggcttgt aagtatactc ataattgctt atgattatac
     6421 ttggatgtgg taaaatggat tggttgggtg gttgtggtct catatcaaat tgggttcggg
     6481 tcaaaattgt aatacttgta catgtcggcc cngggaccga ttgtagccac aattagttat
     6541 taaagtacaa ccttgtgacc atgtttaaga acttaggaca tgcataaact attgcacttt
     6601 aaacataacc accgtaagat ggttgatagt tagttattaa agnataatga tctaacatgt
     6661 ccatagttcc taaacataat ccttatggct ctatttatca aaacccccat aagtacgtaa
     6721 cacatgattt cccatcatat attcattaac aagctagtgt tttgaatata gcagttactt
     6781 ataagacttg gtttcactaa atagagaaat agggaaacca aggttgtggt tacggatcat
     6841 cactttgtat tatgtctact tcgtttattc agtgaaacaa gtgtcataag taattgtggt
     6901 tttattttat ttactatgtg gtgggtttat atggattttt cttcatgtca tttgaaaggg
     6961 taaagctaca cccaaccata actgacctat ctatacgcct gcaggcaaca cctgaactgg
     7021 agttcgggcg tatgaatatt ggaagtcgcc catcaaaacg aaaaccgagt ggtggcattg
     7081 aatcactcag agccattcca tggatctttt catggactca gaccaggttc catctcccag
     7141 tttggctcgg gtttggggcg gcgttcaaac acgccatcca aaaagacagc aagaatctcc
     7201 aaatgcttca agaaatgtac aaaacatggc ctttctttcg ggtcaccatt gatttagttg
     7261 aaatggtgtt tgctaaaggt aacccaggca ttgctgccct gaatgacaag ctccttgttt
     7321 ctgaagatct aaggcccttt ggagaatctt tgagagcaaa ctatgaagaa accaaaaatt
     7381 atcttctcaa ggtacaattt ctcaacattt ataactcacg ctttcggatg ggtcgggcaa
     7441 attggtcaac gtatgtttgg ttgagacggg tcatttttca acactccacc tttatgttat
     7501 aaataatgaa gggttttttc atacaaatca aagttactcc ttataatttt cttcatgtac
     7561 gctccaggtc attttgcaat aggtaaattt ataaaataac ctaaaccaga cacaatcatc
     7621 tacaattatt catgtatgta gaaacaagta caattactaa aacacacaaa tacaaattac
     7681 atgcttttct aaaaaatcat taataaatgc attatttttt aggaatttat gattaaaaag
     7741 tgataatagt ataagaacaa ttgaagtact attatacaac aatcttgctt gtcttacata
     7801 ttaaaaaaga ggccatcatc gagttttaat ctatttgacc ttccatttag cttagttctt
     7861 tattgttctt ttttttgttc catttgatcc gtttgagata acacacatcc caaatcaacc
     7921 agttcataag taaacaagcc gaaactatcc ccacttaatt attatgctaa ccagaaactc
     7981 ttgtcggagt tttagattgc tggacataag gaccttctag agggtgatcc ctacttgaag
     8041 caaggaatca ggctgcgtga tccgtacatc acaaccttga atgtatgcca agcttatacc
     8101 ctaaagagga tccgtgaccc gaactatcat gtgacattaa ggcctcatat ttctaaagaa
     8161 tatgccgctg agccgagcaa accagctgat gagcttatcc acctgaaccc aaccagcgag
     8221 tacgcacccg gtttggagga cacgctcatc ttgaccatga aagggattgc tgctggaatg
     8281 cagaacaccg gttaggttaa ccgttatgca agtatgtatt tatctttttt ttcatgcttt
     8341 tggatgttgt cacatgcctt gtttactgca tgtgttcttg agtgtttgta tgcatcatca
     8401 atctatgcat ttcagaagtt gtactgtgtg catcattgat ggtggtttat ccaataaact
     8461 aaagacttat tgtacacttg actacaagtg attatataaa ttgttgttct ttctacttat
     8521 tttaccatct ttgcagattc attctataga accgcatacc aaatcgactc tatgattaag
     8581 attgaaacgc ttataaaatt ttaactaact tgaagttaaa tatgtctaga catgatatgt
     8641 aggatggtaa taatttcacg cttatagtca tcaaacatat gaaattttgg ttgtgtattt
     8701 gtttttatta gcgatcatta aacactttta atagcgtatc ttttgacacc gaaataggta
     8761 aagacattta cagattatac gtgaatataa aagccgtgta aaatatgaaa agttaaagca
     8821 tgaatagtgt ataggaccaa aaataatttc attgtacttt ataaagctga ctcactcaac
     8881 ataaaaactt tatgaaatat cactcaacat ttcccttgta tttaaaaact ttccgatctc
     8941 actcaacatt tcccttgtat ttaaaaactt tctacttcaa gactcctttg tattccttca
     9001 tttatgaaat at
//