From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Rattus norvegicus di-N-acetylchitobiase mRNA, complete cds.
Date: 29 Dec 92 19:59:26 GMT
Distribution: bionet
Checksum: 16573 4

LOCUS       RATCHITO     1616 bp ss-mRNA            ROD       10-DEC-1992
DEFINITION  Rattus norvegicus di-N-acetylchitobiase mRNA, complete cds.
ACCESSION   M95768
KEYWORDS    chitobiase; di-N-acetylchitobiase.
SOURCE      Rattus norvegicus (library: lambda gt11) liver cDNA to mRNA.
  ORGANISM  Rattus norvegicus
            Eukaryota; Animalia; Chorqata; Vertebrata; Mammalia; Theria;
            Eutheria; Rodentia; Myomorpha; Muridae; Murinae.
REFERENCE   1  (bases 1 to 1616)
  AUTHORS   Fisher,K.J. and Aronson,N.N.Jr.
  TITLE     Cloning and expression of the cDNA sequence encoding the lysosomal
            glycosidase di-N-acetylchitobiase
  JOURNAL   J. Biol. Chem. 267, 19607-19616 (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     sig_peptide     1..69
                     /codon_start=1
                     /note="putative"
     mat_peptide     70..1101
                     /standard_name="chitobiase"
                     /product="di-N-acetylchitobiase"
                     /codon_start=1
                     /note="putative"
     3'UTR           1105..1616
                     /note="putative"
     polyA_signal    1250..1255
                     /note="putative"
     polyA_signal    1591..1596
                     /note="putative"
     CDS             1..1104
                     /note="putative"
                     /product="di-N-acetylchitobiase"
                     /codon_start=1
                     /translation="MALSDLLELTLLLLLPLLERLSAEDCPCSEASLCRPIRHHRDFE
                     VFVFDVGQKTWKSYDWSQITTVAVFGKYDSELMCYAHSKGARVVLKGDVALKDIINPT
                     FRASWIAQKVALAKAQHMDGINIDIEQEVDCSSPEYEALTALVRETTEGFHREIEGSQ
                     VTFDVAWSPKGIDKRCYNYTGIADACDFLFVMSYDEQSQIWSECIAAANAPYNQTLTG
                     YGDYLRMGISPRKLVMGIPWYGYDYICLNLSKDDVCAIAKVPFRGAPCSDAAGHQVPY
                     RVIMKQVNSSVSGSQWNQDQQAPYYNYKDPTGRLHQVWYDNPRSISLKAAFVKHYGLR
                     GIGMWNANCLDYSDDALAREQTEEMWGALRPRL"
BASE COUNT      478 a    330 c    369 g    439 t
ORIGIN      
        1 atggcgctga gtgacctcct ggaactcact ctgctgctgc tgctgcccct gctggagcgg
       61 ctctcggccg aggactgccc gtgctccgag gcttctctct gccgaccgat ccgccaccac
      121 cgcgacttcg aggtctttgt gtttgatgtt ggacagaaaa cctggaaatc ttatgactgg
      181 tcgcagatta cgactgtggc agtttttgga aaatatgact cagaacttat gtgctatgct
      241 cattcgaaag gagccagggt agtgctaaaa ggtgatgtgg ccctgaagga catcattaat
      301 cctaccttca gagcgtcatg gatagcgcaa aaagttgcct tagccaaagc acaacacatg
      361 gatggaatta atatagacat agaacaggaa gttgattgct cgtcacctga atatgaagca
      421 ttgacagctt tggtcagaga aaccacagag ggtttccatc gtgagattga gggatcacag
      481 gtaacttttg atgtagcttg gtcaccaaag ggcatagaca aaaggtgcta taattatact
      541 gggattgcgg atgcctgcga ctttcttttt gtgatgtctt acgatgagca aagtcagatc
      601 tggtcagaat gtattgcagc cgccaatgct ccctacaatc agacattaac tggatatggt
      661 gactacctca ggatgggcat cagccccagg aaacttgtaa tgggtattcc ctggtatggc
      721 tatgattaca tttgcctaaa cctctcaaag gatgatgtct gcgccattgc aaaggtccct
      781 ttcagagggg ctccttgcag tgatgctgca gggcatcagg tgccctacag ggtcatcatg
      841 aagcaagtaa acagttctgt ttctggaagc cagtggaacc aagaccagca agctccgtac
      901 tataattaca aggatcccac tggccgtttg catcaggtgt ggtacgataa cccccggagc
      961 atctcactaa aggcagcatt cgtcaaacac tacggcttgc ggggcattgg catgtggaac
     1021 gcgaactgtc tggactactc agatgacgct ctagccagag agcaaacgga ggaaatgtgg
     1081 ggagccctga gaccacggct gtgatacata cagaggaatg tcttttgtca gacggttaga
     1141 tttgcaggac tggcctgtgt aagcagatct acctcttcaa gagataaaaa tatatatttt
     1201 gtcatgttac cctaacttac ttattggtct actcaataca caggaaagaa ataaagaaac
     1261 tattcatttg tacttggaaa atacatacat atcttaaata tccaggaatg taaaagcaaa
     1321 aacaagtcaa cttactctat taaatattcc tctgtttcag tattataatt atattatatt
     1381 ataattagaa aagttgcttt tagaattcca tgctgaattt ttcctcctgt tattaaaata
     1441 tactgttaaa aaccaatagg gatttttaaa aatgtataac tagtagacaa gtgaagatac
     1501 tggagaaggg agtgcacatg accatagtgt gttacacaca tgtatagact atcatagtaa
     1561 agcccattat ttttataatt aatatatgct aataaaagat gtgtaacttg aaatgg
//