From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Thermococcus litoralis I-TliI endonuclease (I-TliI) gene, complete
Date: 29 Dec 92 20:58:56 GMT
Distribution: bionet
Checksum: 08479 12

LOCUS       THCVDPE      5837 bp ds-DNA             BCT       11-DEC-1992
DEFINITION  Thermococcus litoralis I-TliI endonuclease (I-TliI) gene, complete
            cds and DNA-dependent DNA polymerase (pol) gene, complete cds.
ACCESSION   M74198
KEYWORDS    DNA polymerase; DNA-dependent DNA polymerase; I-TliI endonuclease;
            endonuclease; intervening sequence; pol protein.
SOURCE      Thermococcus litoralis (library: lambda Dash BamHI; lambda gt11;
            lambda Zap EcoRI) DNA.
  ORGANISM  Thermococcus litoralis
            Prokaryotae; Archaeobacteria; Methanobacteriales;
            Methanothermaceae.
REFERENCE   1  (sites)
  AUTHORS   Hodges,R.A., Perler,F.B., Noren,C.J. and Jack,W.E.
  TITLE     Protein splicing removes intervening sequences in an archaea DNA
            polymerase
  JOURNAL   Nucleic Acids Res. 20, 6153-6157 (1992)
  STANDARD  full automatic
REFERENCE   2  (bases 1 to 5837)
  AUTHORS   Perler,F.B., Comb,D.G., Jack,W.E., Moran,L.S., Qiang,B.,
            Kucera,R.B., Benner,J.S., Slatko,B.E., Nwankwo,D.O.,
            Hempstead,S.K., Carlow,C.K. and Jannasch,H.
  TITLE     Intervening sequences in an Archaebacterium DNA polymerase gene
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 89, 5577-5581 (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     misc_feature    1773..3396
                     /standard_name="IVS1; intervening sequence 1"
                     /evidence="EXPERIMENTAL"
     misc_feature    3534..4703
                     /standard_name="IVS2; intervening sequence 2"
                     /evidence="EXPERIMENTAL"
     mat_peptide     3534..(4703.5396)
                     /product="I-TliI endonuclease"
                     /gene="I-TliI"
                     /evidence="EXPERIMENTAL"
                     /codon_start=1
     CDS             291..5399
                     /EC_number="2.7.7.7"
                     /standard_name="Vent DNA polymerase"
                     /gene="pol"
                     /evidence="EXPERIMENTAL"
                     /product="DNA-dependent DNA polymerase"
                     /codon_start=1
                     /translation="MILDTDYITKDGKPIIRIFKKENGEFKIELDPHFQPYIYALLKD
                     DSAIEEIKAIKGERHGKTVRVLDAVKVRKKFLGREVEVWKLIFEHPQDVPAMRGKIRE
                     HPAVVDIYEYDIPFAKRYLIDKGLIPMEGDEELKLLAFDIETFYHEGDEFGKGEIIMI
                     SYADEEEARVITWKNIDLPYVDVVSNEREMIKRFVQVVKEKDPDVIITYNGDNFDLPY
                     LIKRAEKLGVRLVLGRDKEHPEPKIQRMGDSFAVEIKGRIHFDLFPVVRRTINLPTYT
                     LEAVYEAVLGKTKSKLGAEEIAAIWETEESMKKLAQYSMEDARATYELGKEFFPMEAE
                     LAKLIGQSVWDVSRSSTGNLVEWYLLRVAYARNELAPNKPDEEEYKRRLRTTYLGGYV
                     KEPEKGLWENIIYLDFRSLYPSIIVTHNVSPDTLEKEGCKNYDVAPIVGYRFCKDFPG
                     FIPSILGDLIAMRQDIKKKMKSTIDPIEKKMLDYRQRAIKLLANSILPNEWLPIIENG
                     EIKFVKIGEFINSYMEKQKENVKTVENTEVLEVNNLFAFSFNKKIKESEVKKVKALIR
                     HKYKGKAYEIQLSSGRKINITAGHSLFTVRNGEIKEVSGDGIKEGDLIVAPKKIKLNE
                     KGVSINIPELISDLSEEETADIVMTISAKGRKNFFKGMLRTLRWMFGEENRRIRTFNR
                     YLFHLEKLGLIKLLPRGYEVTDWERLKKYKQLYEKLAGSVKYNGNKREYLVMFNEIKD
                     FISYFPQKELEEWKIGTLNGFRTNCILKVDEDFGKLLGYYVSEGYAGAQKNKTGGISY
                     SVKLYNEDPNVLESMKNVAEKFFGKVRVDRNCVSISKKMAYLVMKCLCGALAENKRIP
                     SVILTSPEPVRWSFLEAYFTGDGDIHPSKRFRLSTKSELLANQLVFLLNSLGISSVKI
                     GFDSGVYRVYINEDLQFPQTSREKNTYYSNLIPKEILRDVFGKEFQKNMTFKKFKELV
                     DSGKLNREKAKLLEFFINGDIVLDRVKSVKEKDYEGYVYDLSVEDNENFLVGFGLLYA
                     HNSYYGYMGYPKARWYSKECAESVTAWGRHYIEMTIREIEEKFGFKVLYADSVSGESE
                     IIIRQNGKIRFVKIKDLFSKVDYSIGEKEYCILEGVEALTLDDDGKLVWKPVPYVMRH
                     RANKRMFRIWLTNSWYIDVTEDHSLIGYLNTSKTKTAKKIGERLKEVKPFELGKAVKS
                     LICPNAPLKDENTKTSEIAVKFWELVGLIVGDGNWGGDSRWAEYYLGLSTGKDAEEIK
                     QKLLEPLKTYGVISNYYPKNEKGDFNILAKSLVKFMKRHFKDEKGRRKIPEFMYELPV
                     TYIEAFLRGLFSADGTVTIRKGVPEIRLTNIDADFLREVRKLLWIVGISNSIFAETTP
                     NRYNGVSTGTYSKHLRIKNKWRFAERIGFLIERKQKRLLEHLKSARVKRNTIDFGFDL
                     VHVKKVEEIPYEGYVYDIEVEETHRFFANNILVHNTDGFYATIPGEKPELIKKKAKEF
                     LNYINSKLPGLLELEYEGFYLRGFFVTKKRYAVIDEEGRITTRGLEVVRRDWSEIAKE
                     TQAKVLEAILKEGSVEKAVEVVRDVVEKIAKYRVPLEKLVIHEQITRDLKDYKAIGPH
                     VAIAKRLAARGIKVKPGTIISYIVLKGSGKISDRVILLTEYDPRKHKYDPDYYIENQV
                     LPAVLRILEAFGYRKEDLRYQSSKQTGLDAWLKR"
BASE COUNT     1947 a   1027 c   1336 g   1527 t
ORIGIN      
        1 gaattcgcga taaaatctat tttcttcctc catttttcaa tttcaaaaac gtaagcatga
       61 gccaaacctc tcgccctttc tctgtccttc ccgctaaccc tcttgaaaac tctctccaaa
      121 gcattttttg atgaaagctc acgctcctct atgagggtca gtatatctgc aatgagttcg
      181 tgaagggtta ttctgtagaa caactccatg attttcgatt tggatggggg tttaaaaatt
      241 tggcggaact tttatttaat ttgaactcca gtttatatct ggtggtattt atgatactgg
      301 acactgatta cataacaaaa gatggcaagc ctataatccg aatttttaag aaagagaacg
      361 gggagtttaa aatagaactt gaccctcatt ttcagcccta tatatatgct cttctcaaag
      421 atgactccgc tattgaggag ataaaggcaa taaagggcga gagacatgga aaaactgtga
      481 gagtgctcga tgcagtgaaa gtcaggaaaa aatttttggg aagggaagtt gaagtctgga
      541 agctcatttt cgagcatccc caagacgttc cagctatgcg gggcaaaata agggaacatc
      601 cagctgtggt tgacatttac gaatatgaca taccctttgc caagcgttat ctcatagaca
      661 agggcttgat tcccatggag ggagacgagg agcttaagct ccttgccttt gatattgaaa
      721 cgttttatca tgagggagat gaatttggaa agggcgagat aataatgatt agttatgccg
      781 atgaagaaga ggccagagta atcacatgga aaaatatcga tttgccgtat gtcgatgttg
      841 tgtccaatga aagagaaatg ataaagcgtt ttgttcaagt tgttaaagaa aaagaccccg
      901 atgtgataat aacttacaat ggggacaatt ttgatttgcc gtatctcata aaacgggcag
      961 aaaagctggg agttcggctt gtcttaggaa gggacaaaga acatcccgaa cccaagattc
     1021 agaggatggg tgatagtttt gctgtggaaa tcaagggtag aatccacttt gatcttttcc
     1081 cagttgtgcg aaggacgata aacctcccaa cgtatacgct tgaggcagtt tatgaagcag
     1141 ttttaggaaa aaccaaaagc aaattaggag cagaggaaat tgccgctata tgggaaacag
     1201 aagaaagcat gaaaaaacta gcccagtact caatggaaga tgctagggca acgtatgagc
     1261 tcgggaagga attcttcccc atggaagctg agctggcaaa gctgataggt caaagtgtat
     1321 gggacgtctc gagatcaagc accggcaacc tcgtggagtg gtatctttta agggtggcat
     1381 acgcgaggaa tgaacttgca ccgaacaaac ctgatgagga agagtataaa cggcgcttaa
     1441 gaacaactta cctgggagga tatgtaaaag agccagaaaa aggtttgtgg gaaaatatca
     1501 tttatttgga tttccgcagt ctgtaccctt caataatagt tactcacaac gtatccccag
     1561 atacccttga aaaagagggc tgtaagaatt acgatgttgc tccgatagta ggatataggt
     1621 tctgcaagga ctttccgggc tttattccct ccatactcgg ggacttaatt gcaatgaggc
     1681 aagatataaa gaagaaaatg aaatccacaa ttgacccgat cgaaaagaaa atgctcgatt
     1741 ataggcaaag ggctattaaa ttgcttgcaa acagcatctt acccaacgag tggttaccaa
     1801 taattgaaaa tggagaaata aaattcgtga aaattggcga gtttataaac tcttacatgg
     1861 aaaaacagaa ggaaaacgtt aaaacagtag agaatactga agttctcgaa gtaaacaacc
     1921 tttttgcatt ctcattcaac aaaaaaatca aagaaagtga agtcaaaaaa gtcaaagccc
     1981 tcataagaca taagtataaa gggaaagctt atgagattca gcttagctct ggtagaaaaa
     2041 ttaacataac tgctggccat agtctgttta cagttagaaa tggagaaata aaggaagttt
     2101 ctggagatgg gataaaagaa ggtgacctta ttgtagcacc aaagaaaatt aaactcaatg
     2161 aaaaaggggt aagcataaac attcccgagt taatctcaga tctttccgag gaagaaacag
     2221 ccgacattgt gatgacgatt tcagccaagg gcagaaagaa cttctttaaa ggaatgctga
     2281 gaactttaag gtggatgttt ggagaagaaa atagaaggat aagaacattt aatcgctatt
     2341 tgttccatct cgaaaaacta ggccttatca aactactgcc ccgcggatat gaagttactg
     2401 actgggagag attaaagaaa tataaacaac tttacgagaa gcttgctgga agcgttaagt
     2461 acaacggaaa caagagagag tatttagtaa tgttcaacga gatcaaggat tttatatctt
     2521 acttcccaca aaaagagctc gaagaatgga aaattggaac tctcaatggc tttagaacga
     2581 attgtattct caaagtcgat gaggattttg ggaagctcct aggttactat gttagtgagg
     2641 gctatgcagg tgcacaaaaa aataaaactg gtggtatcag ttattcggtg aagctttaca
     2701 atgaggaccc taatgttctt gagagcatga aaaatgttgc agaaaaattc tttggcaagg
     2761 ttagagttga cagaaattgc gtaagtatat caaagaagat ggcatactta gttatgaaat
     2821 gcctctgtgg agcattagcc gaaaacaaga gaattccttc tgttatactc acctctcccg
     2881 aaccggtacg gtggtcattt ttagaggcgt attttacagg cgatggagat atacatccat
     2941 caaaaaggtt taggctctca acaaaaagcg agctccttgc aaatcagctt gtgttcttgc
     3001 tgaactcttt gggaatatcc tctgtaaaga taggctttga cagtggggtc tatagagtgt
     3061 atataaatga agacctgcaa tttccacaaa cgtctaggga gaaaaacaca tactactcta
     3121 acttaattcc caaagagatc cttagggacg tgtttggaaa agagttccaa aagaacatga
     3181 cgttcaagaa atttaaagag cttgttgact ctggaaaact taacagggag aaagccaagc
     3241 tcttggagtt cttcattaat ggagatattg tccttgacag agtcaaaagt gttaaagaaa
     3301 aggactatga agggtatgtc tatgacctaa gcgttgagga taacgagaac tttcttgttg
     3361 gttttggttt gctctatgct cacaacagct attacggcta tatggggtat cctaaggcaa
     3421 gatggtactc gaaggaatgt gctgaaagcg ttaccgcatg ggggagacac tacatagaga
     3481 tgacgataag agaaatagag gaaaagttcg gctttaaggt tctttatgcg gacagtgtct
     3541 caggagaaag tgagatcata ataaggcaaa acggaaagat tagatttgtg aaaataaagg
     3601 atcttttctc taaggtggac tacagcattg gcgaaaaaga atactgcatt ctcgaaggtg
     3661 ttgaagcact aactctggac gatgacggaa agcttgtctg gaagcccgtc ccctacgtga
     3721 tgaggcacag agcgaataaa agaatgttcc gcatctggct gaccaacagc tggtatatag
     3781 atgttactga ggatcattct ctcataggct atctaaacac gtcaaaaacg aaaactgcca
     3841 aaaaaatcgg ggaaagacta aaggaagtaa agccttttga attaggcaaa gcagtaaaat
     3901 cgctcatatg cccaaatgca ccgttaaagg atgagaatac caaaactagc gaaatagcag
     3961 taaaattctg ggagctcgta ggattgattg taggagatgg aaactggggt ggagattctc
     4021 gttgggcaga gtattatctt ggactttcaa caggcaaaga tgcagaagag ataaagcaaa
     4081 aacttctgga acccctaaaa acttatggag taatctcaaa ctattaccca aaaaacgaga
     4141 aaggggactt caacatcttg gcaaagagcc ttgtaaagtt tatgaaaagg cactttaagg
     4201 acgaaaaagg aagacgaaaa attccagagt tcatgtatga gcttccggtt acttacatag
     4261 aggcatttct acgaggactg ttttcagctg atggtactgt aactatcagg aagggagttc
     4321 cagagatcag gctaacaaac attgatgctg actttctaag ggaagtaagg aagcttctgt
     4381 ggattgttgg aatttcaaat tcaatatttg ctgagactac tccaaatcgc tacaatggtg
     4441 tttctactgg aacctactca aagcatctaa ggatcaaaaa taagtggcgt tttgctgaaa
     4501 ggataggctt tttaatcgag agaaagcaga agagactttt agaacattta aaatcagcga
     4561 gggtaaaaag gaataccata gattttggct ttgatcttgt gcatgtgaaa aaagtcgaag
     4621 agataccata cgagggttac gtttatgaca ttgaagtcga agagacgcat aggttctttg
     4681 caaacaacat cctggtacac aatactgacg gcttttatgc cacaataccc ggggaaaagc
     4741 ctgaactcat taaaaagaaa gccaaggaat tcctaaacta cataaactcc aaacttccag
     4801 gtctgcttga gcttgagtat gagggctttt acttgagagg attctttgtt acaaaaaagc
     4861 gctatgcagt catagatgaa gagggcagga taacaacaag gggcttggaa gtagtaagga
     4921 gagattggag tgagatagct aaggagactc aggcaaaggt tttagaggct atacttaaag
     4981 agggaagtgt tgaaaaagct gtagaagttg ttagagatgt tgtagagaaa atagcaaaat
     5041 acagggttcc acttgaaaag cttgttatcc atgagcagat taccagggat ttaaaggact
     5101 acaaagccat tggccctcat gtcgcgatag caaaaagact tgccgcaaga gggataaaag
     5161 tgaaaccggg cacaataata agctatatcg ttctcaaagg gagcggaaag ataagcgata
     5221 gggtaatttt acttacagaa tacgatccta gaaaacacaa gtacgatccg gactactaca
     5281 tagaaaacca agttttgccg gcagtactta ggatactcga agcgtttgga tacagaaagg
     5341 aggatttaag gtatcaaagc tcaaaacaaa ccggcttaga tgcatggctc aagaggtagc
     5401 tctgttgctt tttagtccaa gtttctccgc gagtctctct atctctcttt tgtattctgc
     5461 tatgtggttt tcattcacta ttaagtagtc cgccaaagcc ataacgcttc caattccaaa
     5521 cttgagctct ttccagtctc tggcctcaaa ttcactccat gtttttggat cgtcgcttct
     5581 ccctcttctg ctaagcctct cgaatctttt tcttggcgaa gagtgtacag ctatgatgat
     5641 tatctcttcc tctggaaacg catctttaaa cgtctgaatt tcatctagag acctcactcc
     5701 gtcgattata actgccttgt acttctttag tagttctttt acctttggga tcgttaattt
     5761 tgccacggca ttgtccccaa gctcctgcct aagctgaatg ctcacactgt tcataccttc
     5821 gggagttctt gggatcc
//