From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Mouse mRNA for DNA topoisomerase I.
Date: 27 Jan 93 05:50:52 GMT
Distribution: bionet
Checksum: 37454 7

LOCUS       MUSTOPI      3512 bp ss-mRNA            ROD       27-JAN-1993
DEFINITION  Mouse mRNA for DNA topoisomerase I.
ACCESSION   D10061
KEYWORDS    DNA topoisomerase I.
SOURCE      Mouse (strain Balb/c; cell line embryonic-fibroblast 3T3, lymphoma
            RL-12), cDNA to mRNA.
  ORGANISM  Mus musculus
            Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Mammalia;
            Theria; Eutheria; Rodentia; Myomorpha; Muridae; Murinae.
REFERENCE   1  (bases 1 to 3512)
  AUTHORS   Koiwai,O.
  TITLE     Molecular cloning of mouse cDNA and chromosome
  JOURNAL   Unpublished (1991)
  STANDARD  full automatic
COMMENT     Submitted (10-Dec-1991) to DDBJ by: Osamu Koiwai Biichemistry Unit
            Aichi Cancer Center Research Institute 1-1 Kanokoden, Chikusa-ku
            Nagoya 464 Japan Phone:  052-762-6111 x825 Fax:    052-763-5233
               Sequence updated (19-JAN-1993) by : Osamu Koiwai.
FEATURES             Location/Qualifiers
     CDS             1..2304
                     /gene="Top I"
                     /product="DNA topoisomerase I"
                     /codon_start=1
                     /translation="MSGDHLHNDSQIEADFRLNDSHKHKDKHKDREHRHKEHKKDKDK
                     DREKSKHSNSEHKDSEKKHKEKEKTKHKDGSSEKHKDKHKDRDKERRKEEKIRAAGDA
                     KIKKEKENGFSSPPRIKDDPEDDGYFAPPKEDIKPLKRLRDEDDADYKPKKIKTEDIK
                     KEKKRKSEEEEDGKLKKPKNKDKDKKVAEPDNKKKKPKKEEEQKWKWWEEERYPEGIK
                     WKFLEHKGPVFAPPYEPLPESVKFYYDGKVMKLSPKAEEVATFFAKMLDHEYTTKEIF
                     RKNFFKDWRKEMTNDGKNTITNLSKCDFTQMSQYFKAQSEARKQMSKEEKLKIKEENE
                     KLLKEYGFCVMDNHRERIANFKIEPPGLFRGRGNHPKMGMLKRRIMPEDIIINCSKDA
                     KVPSPPPGHKWKEVRHDNKVTWLVSWTENIQGSIKYIMLNPSSRIKGEKDWQKYETAR
                     RLKKCVDKIRNQYREDWKSKEMKVRQRAVALYFIDKLALRAGNEKEEGETADTVGCCS
                     LRVEHINLHPELDGQEYVVEFDFPGKDSIRYYNKVPVEKRVFKNLQLFMENKQPEDDL
                     FDRLNTGILNKHLQDLMEGLTAKVFRTYNASITLQQQLKELTAPDENVPAKILSYNRA
                     NRAVAILCNHQRAPPKTFEKSMMNLQSKIDAKKDQLADARRDLKSAKADAKVMKDAKT
                     KKVVESKKKAVQRLEEQLMKLEVQATDREENKQIALGTSKLNYLDPRITVAWCKKWGV
                     PIEKIYNKTQREKFAWAIDMTDEDYEF"
BASE COUNT     1244 a    611 c    787 g    870 t
ORIGIN      Chromosome 2
        1 atgagtgggg accacctcca caacgattcc cagatcgaag cggatttccg attgaatgat
       61 tctcacaaac acaaagataa acacaaagat cgagaacacc ggcataagga gcacaagaag
      121 gataaggata aggaccggga aaagtctaag catagcaaca gtgaacataa agattctgaa
      181 aagaaacaca aagagaaaga gaaaaccaaa cacaaagatg gcagctcaga aaaacataaa
      241 gacaaacata aagacagaga caaggaaaga cgaaaggagg aaaagattag agctgctggg
      301 gatgcaaaaa taaagaagga aaaggaaaat ggcttctcta gtccgccacg aattaaagat
      361 gaccctgagg atgatggcta ttttgctcct ccaaaagagg atattaagcc attgaagagg
      421 cttcgagatg aagatgatgc tgattataaa cctaagaaaa ttaagacaga agatatcaag
      481 aaggagaaga aaagaaaatc agaagaggaa gaggatggta aacttaaaaa gcccaagaat
      541 aaagataaag ataaaaaagt tgctgagcca gataataaga aaaagaagcc gaaaaaggaa
      601 gaggaacaga agtggaaatg gtgggaagaa gaacgttatc cagaaggcat caaatggaaa
      661 ttcctagagc ataaagggcc tgtcttcgct ccaccatatg agcctctgcc agagagtgtc
      721 aagttttact atgatggtaa agttatgaag ctgagtccta aagcagaaga agtagctaca
      781 ttctttgcaa aaatgcttga ccacgaatat actactaagg aaatattcag gaaaaatttc
      841 tttaaagatt ggagaaagga aatgactaat gacggaaaaa atacgattac caacctaagc
      901 aaatgcgact ttacacagat gagccagtat ttcaaagcgc agtcagaagc tcggaaacag
      961 atgagcaagg aagaaaaatt gaaaatcaaa gaagaaaatg aaaagttatt gaaagaatac
     1021 ggcttttgtg taatggataa tcatagagag cggattgcca acttcaagat agagcctccg
     1081 gggcttttcc gaggccgagg gaaccacccc aagatgggta tgctgaagag aaggatcatg
     1141 cctgaggaca tcatcatcaa ctgtagcaaa gacgcaaagg ttccttctcc ccctcctgga
     1201 cataaatgga aggaagtccg acatgataac aaggttactt ggctggtctc ctggacagag
     1261 aatatccaag gttctatcaa atatatcatg ctgaatccca gttcacgaat caagggtgag
     1321 aaagactggc agaagtatga gactgcgcgg cgtctgaaga agtgtgtgga caagatccga
     1381 aatcagtatc gggaagactg gaagtccaaa gagatgaaag ttcgtcagag agctgtagca
     1441 ctgtacttca ttgataagct tgctctgaga gcaggcaatg agaaggaaga aggagagacg
     1501 gcagacactg tgggctgctg ttcacttaga gtggaacaca tcaatttaca cccagaattg
     1561 gatggtcagg agtatgtggt ggaatttgac tttcctggga aggactcaat cagatactat
     1621 aacaaagtcc cagttgagaa acgagttttt aaaaacttac aactgtttat ggagaacaag
     1681 cagcctgagg acgatctttt tgatcgactt aatactggta ttctaaataa acatcttcag
     1741 gatctcatgg agggcttgac tgccaaggtg ttccgtacct acaatgcctc catcacacta
     1801 cagcagcagc ttaaagagct cacagcccct gatgagaatg taccagcgaa gattctatct
     1861 tataaccgtg ccaatcgagc tgttgcaatt ctttgtaacc accagagggc gccaccaaag
     1921 acctttgaga agtcaatgat gaacttgcag tctaagattg atgccaagaa agatcagtta
     1981 gcagatgctc gaagggacct gaaaagtgct aaggctgatg ccaaagtcat gaaggatgca
     2041 aagaccaaga aggtagtaga gtcaaaaaag aaggctgtac agagactaga agagcagttg
     2101 atgaagctgg aagttcaagc cacagatcga gaggagaaca aacaaattgc cttggggacc
     2161 tccaaactca attatctgga ccctaggatc acagtggctt ggtgcaaaaa gtggggggtc
     2221 ccaattgaga agatttacaa caaaacccag agggagaagt ttgcttgggc cattgatatg
     2281 actgatgagg actacgagtt ttgaccagtc tcggcagggg ttctgtgaaa aggaacagtg
     2341 tggtttgggg aagatggata aactgagcct cacttcccct cacacccgag ggagagaggc
     2401 cacaagtctt aacaaaccaa catctttgca aaaagataga cctggagata ctataaggga
     2461 gagctgagcc agttgtccta tggacaactt atttaaaaat atttcagata tcaaaattct
     2521 agctgtatga tttgttttga attttgtttt tactttcaag agagcaagtg gatgggaatt
     2581 tgtcagcatc ctgccaggca aattcagttt gcctgcaatg tttggattct ctcagctact
     2641 gtatgtgaag tctgattata ttggtgcgtt tttacagtta gggttttgca ataacttcta
     2701 tattttaata gaaacaaatt cctaaactcc ctcctctccc ctatttcagg aatttaaaat
     2761 taagtagaac aaaacaaaaa cccagcgcac ctattacagc tgtcattcta tcgtcatggg
     2821 aatcagtttt cattaaactt gaagcagttg tgacattggc agtgttttgg ttcagacacc
     2881 tattcacaga aaagcatgat gggaaaatat ttcctgactt gagtgttcct ttttaaatgt
     2941 gaatttttat ttcgttttaa ttattttaaa atatttaagc cttcttcttg atcttaaaga
     3001 tcgtgtagct tggggttggg gagggatgaa gggcgagtga gtctaaggat aatgtaataa
     3061 tcagtgactg aaaccatttt cccatcatcc tttgttctga ccattctttg taccctttaa
     3121 gagatccatc tttttcttgg aaaccccagt ctttcatttg aaagatgtta ttgtataaaa
     3181 acttccacag gtcaataaat ttagaggaaa atgagcattt ggtccaagaa aaacaaaaac
     3241 caaaacaaaa caaaaaagga aaaataatca agattttagg gcttttattt tttcttttgt
     3301 aattgtgtaa aaaatggaaa aaaaaacata aaaaggaaaa ttttaatgtg aagacttttt
     3361 ttttgctata atcattagtt ttagaggcat tgttagttag tgtgtgtgca gagtccattc
     3421 ccacatcttt cctcaagtat cttctatttt tatcatgaat tcccttttaa tcaactgtag
     3481 gttatttaaa ataaactcct acaacttaac gg
//