From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Winter squash mRNA for 1-aminocyclopropane-1-carboxylate synthase
Date: 29 Dec 92 00:50:07 GMT
Distribution: bionet
Checksum: 24934 5

LOCUS       CUCACCA      1638 bp ss-mRNA            PLN       04-DEC-1992
DEFINITION  Winter squash mRNA for 1-aminocyclopropane-1-carboxylate synthase
            (EC 4.4.1.14).
ACCESSION   D01033
KEYWORDS    1-aminocyclopropane-1-carboxylate; accA gene.
SOURCE      Winter squash (cv. Ebisu) seedling, stem, cDNA to mRNA, clone
            lambda CMA101.
  ORGANISM  Cucurbita maxima
            Eukaryota; Plantae; Embryobionta; Magnoliophyta; Magnoliopsida;
            Dilleniidae; Violales; Cucurbitaceae.
REFERENCE   1  (sites)
  AUTHORS   Nakajima,N., Mori,H., Yamazaki,K. and Imaseki,H.
  TITLE     Molecular cloning and sequence of a complementary DNA encoding
            1-aminocyclopropane-1-carboxylate synthase induced by tissue
            wounding
  JOURNAL   Plant Cell Physiol. 31, 1021-1029 (1990)
  STANDARD  full automatic
REFERENCE   2  (bases 1 to 1638)
  AUTHORS   Nakagawa,N., Mori,H., Yamazaki,K. and Imaseki,H.
  TITLE     Cloning of a complementary DNA for auxin-induced
            1-aminocyclopropane-1-carboxylate synthase and differential
            expression of the gene by auxin and wounding
  JOURNAL   Plant Cell Physiol. 32, 1153-1163 (1991)
  STANDARD  full automatic
COMMENT     Data kindly submitted in computer readable form by: Hidemasa
            Imaseki School of Agricultural Sciences Nagoya University
            Chikusa-ku, Nagoya Aichi 464-01 Japan Phone:  052-781-5111 x6268
            Fax:    052-781-4447 Telex:   442-2120

               [1]  sites.
FEATURES             Location/Qualifiers
     CDS             92..1519
                     /note="1-aminocyclopropane-1-carboxylate synthase"
                     /codon_start=1
                     /translation="MKMLSTKATCNSHGQDSSYFLGWEAYENNPFHHTSNPNGIIQMG
                     LAENQLSFDLLESWLSKNPDAASFKRDGKSIFRELALFQDYHGLPAFKKALVEFMAEI
                     RGNKVSFEANNIVLTAGATSANETLMFCLAEAGDAFLLPTPYYPGFDRDLKWRTGVEI
                     VPIHCTSSNGFQITQSALEQAYKDAQTRNLRVKGVLVTNPSNPLGTTMNRDELNLVFD
                     FITSKGIHLISDEIYSGTVFGSPGFVSAMEVLKERSSEDEEVWKRVHIVYSLSKDLGL
                     PGFRVGAIYSNDDMVVAAATKMSSFGLVSSQTQYLLSAMLSDKKFTISYISENQKRLK
                     QRQKMLVSGLQKAGINCLDSNAGLFCWVDMRHLLESDKFESELELWKKIVYEVGLNIS
                     PGSSCHCTEPGWFRVCFANMSESTLKLAVRRLKSFVTELRSTTTSNHRNHDNKICKNI
                     KKNIFTKWVFRQSAQEANRKMQAER"
BASE COUNT      471 a    383 c    333 g    451 t
ORIGIN      
        1 ctctttctct ctctccaaaa tttgccaatc ttcttcctca taaatctcac caccttataa
       61 cataacctca tacaaatttg ttcatacaaa tatgaagatg ctatccacaa aggccacatg
      121 caactcccac ggtcaagatt cctcctactt cttgggatgg gaggcttatg aaaacaatcc
      181 cttccatcac acttctaatc ccaacggcat aattcaaatg ggtctcgctg agaatcagct
      241 atccttcgac cttcttgaat catggctctc taagaatcca gacgccgcca gcttcaaacg
      301 tgatggcaaa tccattttca gagagctcgc cctcttccaa gattaccatg gcttacccgc
      361 attcaaaaag gcattggttg aatttatggc ggaaattaga ggaaacaaag tatcatttga
      421 ggctaataac atagtcctca cagctggagc cacttcagca aatgagactc ttatgttctg
      481 cctcgctgaa gctggcgacg cttttctcct cccaacacca tactatccag gatttgatag
      541 agatctcaaa tggagaaccg gcgttgagat tgtgccaatt cattgcacta gctccaatgg
      601 cttccaaatc acccaatccg ctcttgaaca agcctacaaa gacgcccaaa ctcgcaacct
      661 acgcgtcaaa ggcgtgttgg tcacaaaccc atctaaccca ttaggcacca caatgaaccg
      721 ggatgaactc aaccttgtct ttgatttcat aacctccaaa ggaattcact tgatcagcga
      781 cgagatctac tccggcaccg tgtttgggtc cccgggattt gtgagtgcga tggaagtgct
      841 caaggagagg agtagcgaag acgaggaggt ttggaagaga gtgcatattg tttacagttt
      901 gtctaaagat ttgggtcttc cagggtttcg cgttggtgca atttactcaa atgatgacat
      961 ggtggtggct gcggctacca aaatgtcgag ctttgggttg gtctcatctc aaacacaata
     1021 ccttctctcc gctatgctct ccgacaagaa attcacgata agctacattt cggagaatca
     1081 aaaaaggcta aaacaaagac aaaaaatgct tgtgtccggc ttacaaaagg ccggtattaa
     1141 ctgcctggat agcaacgccg ggctgttttg ttgggtggac atgaggcatt tgttggaatc
     1201 cgacaagttc gaatccgagc tagagctgtg gaagaagatt gtgtacgagg tgggtttaaa
     1261 catttcgcct ggatcatcat gccattgcac tgaaccaggg tggttccgtg tttgctttgc
     1321 caacatgtcg gaatcaacat tgaaactcgc cgtccgaaga ttgaagtcgt ttgtaacaga
     1381 attgaggtcc actactactt caaatcatcg taatcatgac aataaaattt gcaagaacat
     1441 caagaaaaac attttcacca aatgggtttt ccggcaatca gctcaagaag ccaaccgcaa
     1501 gatgcaagct gaacgatagc ctggtcgtat gaacataata ttcatcatcc atatttgggg
     1561 gatttttttt ttttaattta tttcttggat tcttttaatc tctcgttgaa atattcaaat
     1621 taaaaatttt agtcttgt
//