From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Maize mRNA for branching enzyme-I (BE-I).
Date: 5 Nov 92 23:12:44 GMT
Distribution: bionet
Checksum: 22254 8

LOCUS       MZEBEI       2763 bp ss-mRNA            PLN       15-OCT-1992
DEFINITION  Maize mRNA for branching enzyme-I (BE-I).
ACCESSION   D11081
KEYWORDS    branching enzyme-I.
SOURCE      Zea mays L. (inbred Oh43), cDNA to mRNA.
  ORGANISM  Zea mays
            Eukaryota; Plantae; Embryobionta; Magnoliophyta; Liliopsida;
            Commelinidae; Cyperales; Poaceae.
REFERENCE   1  (bases 1 to 2763)
  AUTHORS   Baba,T., Kimura,K., Mizuno,K., Etoh,H., Ishida,Y., Shida,O. and
            Arai,Y.
  TITLE     Sequence conservation of the catalytic regions of Amylolytic
            enzymes in maize branching enzyme-I
  JOURNAL   Biochem. Biophys. Res. Commun. 181, 87-94 (1992)
  STANDARD  full automatic
COMMENT     Submitted (30-APR-1992) to DDBJ by: Tadashi Baba Institute of
            Applied Biochemistry University of Tsukuba Tsukuba, Ibaraki 305
            Japan Phone:  0298-53-6632 Fax:    0298-53-6632.
FEATURES             Location/Qualifiers
     transit_peptide 2..190
     transit_peptide 2..190
     mat_peptide     191..2467
                     /EC_number="2.4.1.18"
                     /product="branching enzyme-I"
                     /codon_start=1
     mat_peptide     191..2467
                     /EC_number="2.4.1.18"
                     /product="branching enzyme-I"
                     /codon_start=1
     polyA_signal    2734..2739
     polyA_signal    2734..2739
     CDS             <1..2470
                     /product="branching enzyme-I precursor"
                     /codon_start=2
                     /translation="LCLVSPSSSPTPLPPPRRSRSHADRAAPPGIAGGGNVRLSVLSV
                     QCKARRSGVRKVKSKFATAATVQEDKTMATAKGDVDHLPIYDLDPKLEIFKDHFRYRM
                     KRFLEQKGSIEENEGSLESFSKGYLKFGINTNEDGTVYREWAPAAQEAELIGDFNDWN
                     GANHKMEKDKFGVWSIKIDHVKGKPAIPHNSKVKFRFLHGGVWVDRIPALIRYATVDA
                     SKFGAPYDGVHWDPPASERYTFKHPRPSKPAAPRIYEAHVGMSGEKPAVSTYREFADN
                     VLPRIRANNYNTVQLMAVMEHSYYASFGYHVTNFFAVSSRSGTPEDLKYLVDKAHSLG
                     LRVLMDVVHSHASNNVTDGLNGYDVGQSTQESYFHAGDRGYHKLWDSRLFNYANWEVL
                     RFLLSNLRYWLDEFMFDGFRFDGVTSMLYHHHGINVGFTGNYQEYFSLDTAVDAVVYM
                     MLANHLMHKLLPEATVVAEDVSGMPVLCRPVDEGGVGFDYRLAMAIPDRWIDYLKNKD
                     DSEWSMGEIAHTLTNRRYTEKCIAYAESHDQSIVGDKTIAFLLMDKEMYTGMSDLQPA
                     SPTIDRGIALQKMIHFITMALGGDGYLNFMGNEFGHPEWIDFPREGNNWSYDKCRRQW
                     SLVDTDHLRYKYMNAFDQAMNALDERFSFLSSSKQIVSDMNDEEKVIVFERGDLVFVF
                     NFHPKKTYEGYKVGCDLPGKYRVALDSDALVFGGHGRVGHDVDHFTSPEGVPGVPETN
                     FNNRPNSFKVLSPPRTCVAYYRVDEAGAGRRLHAKAETGKTSPAESIDVKASRASSKE
                     DKEATAGGKKGWKFARQPSDQDTK"
     CDS             <1..2470
                     /product="branching enzyme-I precursor"
                     /codon_start=2
                     /translation="LCLVSPSSSPTPLPPPRRSRSHADRAAPPGIAGGGNVRLSVLSV
                     QCKARRSGVRKVKSKFATAATVQEDKTMATAKGDVDHLPIYDLDPKLEIFKDHFRYRM
                     KRFLEQKGSIEENEGSLESFSKGYLKFGINTNEDGTVYREWAPAAQEAELIGDFNDWN
                     GANHKMEKDKFGVWSIKIDHVKGKPAIPHNSKVKFRFLHGGVWVDRIPALIRYATVDA
                     SKFGAPYDGVHWDPPASERYTFKHPRPSKPAAPRIYEAHVGMSGEKPAVSTYREFADN
                     VLPRIRANNYNTVQLMAVMEHSYYASFGYHVTNFFAVSSRSGTPEDLKYLVDKAHSLG
                     LRVLMDVVHSHASNNVTDGLNGYDVGQSTQESYFHAGDRGYHKLWDSRLFNYANWEVL
                     RFLLSNLRYWLDEFMFDGFRFDGVTSMLYHHHGINVGFTGNYQEYFSLDTAVDAVVYM
                     MLANHLMHKLLPEATVVAEDVSGMPVLCRPVDEGGVGFDYRLAMAIPDRWIDYLKNKD
                     DSEWSMGEIAHTLTNRRYTEKCIAYAESHDQSIVGDKTIAFLLMDKEMYTGMSDLQPA
                     SPTIDRGIALQKMIHFITMALGGDGYLNFMGNEFGHPEWIDFPREGNNWSYDKCRRQW
                     SLVDTDHLRYKYMNAFDQAMNALDERFSFLSSSKQIVSDMNDEEKVIVFERGDLVFVF
                     NFHPKKTYEGYKVGCDLPGKYRVALDSDALVFGGHGRVGHDVDHFTSPEGVPGVPETN
                     FNNRPNSFKVLSPPRTCVAYYRVDEAGAGRRLHAKAETGKTSPAESIDVKASRASSKE
                     DKEATAGGKKGWKFARQPSDQDTK"
BASE COUNT      719 a    585 c    737 g    722 t
ORIGIN      
        1 gctgtgcctc gtgtcgccct cttcctcgcc gactccgctt ccgccgccgc ggcgctctcg
       61 ctcgcatgct gatcgggcgg caccgccggg gatcgcgggt ggcggcaatg tgcgcctgag
      121 tgtgttgtct gtccagtgca aggctcgccg gtcaggggtg cggaaggtca agagcaaatt
      181 cgccactgca gctactgtgc aagaagataa aactatggca actgccaaag gcgatgtcga
      241 ccatctcccc atatacgacc tggaccccaa gctggagata ttcaaggacc atttcaggta
      301 ccggatgaaa agattcctag agcagaaagg atcaattgaa gaaaatgagg gaagtcttga
      361 atctttttct aaaggctatt tgaaatttgg gattaataca aatgaggatg gaactgtata
      421 tcgtgaatgg gcacctgctg cgcaggaggc agagcttatt ggtgacttca atgactggaa
      481 tggtgcaaac cataagatgg agaaggataa atttggtgtt tggtcgatca aaattgacca
      541 tgtcaaaggg aaacctgcca tccctcacaa ttccaaggtt aaatttcgct ttctacatgg
      601 tggagtatgg gttgatcgta ttccagcatt gattcgttat gcgactgttg atgcctctaa
      661 atttggagct ccctatgatg gtgttcattg ggatcctcct gcttctgaaa ggtacacatt
      721 taagcatcct cggccttcaa agcctgctgc tccacgtatc tatgaagccc atgtaggtat
      781 gagtggtgaa aagccagcag taagcacata tagggaattt gcagacaatg tgttgccacg
      841 catacgagca aataactaca acacagttca gttgatggca gttatggagc attcgtacta
      901 tgcttctttc gggtaccatg tgacaaattt ctttgcggtt agcagcagat caggcacacc
      961 agaggacctc aaatatcttg ttgataaggc acacagtttg ggtttgcgag ttctgatgga
     1021 tgttgtccat agccatgcaa gtaataatgt cacagatggt ttaaatggct atgatgttgg
     1081 acaaagcacc caagagtcct attttcatgc gggagataga ggttatcata aactttggga
     1141 tagtcggctg ttcaactatg ctaactggga ggtattaagg tttcttcttt ctaacctgag
     1201 atattggttg gatgaattca tgtttgatgg cttccgattt gatggagtta catcaatgct
     1261 gtatcatcac catggtatca atgtggggtt tactggaaac taccaggaat atttcagttt
     1321 ggacacagct gtggatgcag ttgtttacat gatgcttgca aaccatttaa tgcacaaact
     1381 cttgccagaa gcaactgttg ttgctgaaga tgtttcaggc atgccggtcc tttgccggcc
     1441 agttgatgaa ggtggggttg ggtttgacta tcgcctggca atggctatcc ctgatagatg
     1501 gattgactac ctgaagaata aagatgactc tgagtggtcg atgggtgaaa tagcgcatac
     1561 tttgactaac aggagatata ctgaaaaatg catcgcatat gctgagagcc atgatcagtc
     1621 tattgttggc gacaaaacta ttgcatttct cctgatggac aaggaaatgt acactggcat
     1681 gtcagacttg cagcctgctt cacctacaat tgatcgaggg attgcactcc aaaagatgat
     1741 tcacttcatc acaatggccc ttggaggtga tggctacttg aattttatgg gaaatgagtt
     1801 tggtcaccca gaatggattg actttccaag agaagggaac aactggagct atgataaatg
     1861 cagacgacag tggagccttg tggacactga tcacttgcgg tacaagtaca tgaatgcgtt
     1921 tgaccaagcg atgaatgcgc tcgatgagag attttccttc ctttcgtcgt caaagcagat
     1981 cgtcagcgac atgaacgatg aggaaaaggt tattgtcttt gaacgtggag atttagtttt
     2041 tgttttcaat ttccatccca agaaaactta cgagggctac aaagtgggat gcgatttgcc
     2101 tgggaaatac agagtagccc tggactctga tgctctggtc ttcggtggac atggaagagt
     2161 tggccacgac gtggatcact tcacgtcgcc tgaaggggtg ccaggggtgc ccgaaacgaa
     2221 cttcaacaac cggccgaact cgttcaaagt cctttctccg ccccgcacct gtgtggctta
     2281 ttaccgtgta gacgaagcag gggctggacg acgtcttcac gcgaaagcag agacaggaaa
     2341 gacgtctcca gcagagagca tcgacgtcaa agcttccaga gctagtagca aagaagacaa
     2401 ggaggcaacg gctggtggca agaagggatg gaagtttgcg cggcagccat ccgatcaaga
     2461 taccaaatga agccacgagt ccttggtgag gactggactg gctgccggcg ccctgttagt
     2521 agtcctgctc tactggacta gccgccgctg gcgcccttgg aacggtcctt tcctgtagct
     2581 tgcaggcgac tggtgtctca tcaccgagca ggcaggcact gcttgtatag cttttctaga
     2641 ataataatca gggatggatg gatggtgtgt attggctatc tggctagacg tgcatgtgcc
     2701 cagtttgtat gtacaggagc agttcccgtc cagaataaaa aaaaacttgt tggggggttt
     2761 ttc
//