HDTenEightyP/NetNews huggingface dataset:
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Escherichia coli polysaccaride antigen chain regulator gene.
Date: 18 Aug 92 10:00:42 GMT
Distribution: bionet
Checksum: 21925     3

LOCUS       ECOREGPOL    1330 bp ds-DNA             BCT       18-AUG-1992
DEFINITION  Escherichia coli polysaccaride antigen chain regulator gene.
ACCESSION   M89934
KEYWORDS    .
SOURCE      Escherichia coli DNA.
  ORGANISM  Escherichia coli
            Prokaryotae; Gram-negative facultatively anaerobic rods;
            Enterobacteriaceae.
REFERENCE   1  (bases 1 to 1330)
  AUTHORS   Batchelor,R.A., Alifano,P., Biffali,E., Hull,S.I. and Hull,R.A.
  TITLE     Nucleotide sequences of the genes regulating O-polysacharride
            antigen chain length (rol) from Escherichia coli and Salmonella
            typhimurium: Protein homology and functional complementation
  JOURNAL   J. Bacteriol. 174, 5228-5236 (1992)
  STANDARD  simple staff_entry
BASE COUNT      364 a    281 c    333 g    352 t
ORIGIN      44 min.
        1 atcagggcta tttacgccct gattgtcttt tgttacctcc gcaataattc attattttta
       61 tcacttatcc tatagcattc acggggatta tcgctaaact atgcggactt ggaaatttcc
      121 gtcagttagg gtaatgatga gagtagaaaa taataatgtt tctgggcaaa accatgaccc
      181 ggaacagatt gatttgattg atttactagt gcagttgtgg cgtggcaaga tgacaattat
      241 catttccgtc attgtggcta ttgccctggc tattggttat ttggcagtag cgaaggagaa
      301 atggacgtca acagcaatta tcactcagcc cgacgtgggg caaattgctg gctataacaa
      361 tgccatgaat gttatctatg gtcaggctgc accgaaagta tcggatttgc aggagacgtt
      421 aattggtcgc ttcagttctg ccttctctgc attagcagaa acgctggata atcaggaaga
      481 gccagaaaaa cttaccatcg aaccttctgt taagaaccag caattaccat tgactgtttc
      541 ttatgttggg caaactgcag agggcgcaca aatgaagttg gcccaataca ttcagcaagt
      601 tgatgataaa gtgaatcaag agctagaaaa ggatctcaag gacaacattg ctctgggacg
      661 gaaaaacttg caggactctt taagaaccca ggaagtggtc gcgcaggagc agaaagatct
      721 gcgtatccgt cagattcagg aagcgttgca gtatgcgaat caggcgcagg tgacaaagcc
      781 gcagattcaa cagacacagg acgtaaccca ggatactatg tttcttttgg ggagcgaagc
      841 gctggagtcg atgattaagc acgaagcgac ccgtccgttg gtgttctcat caaactacta
      901 tcagactcgt caaaacctgc tggatattga caatcttgac gttgataaac ttgatattca
      961 tgcttaccgc tatgtaatga aaccgacgtt acctattcgt cgcgatagcc cgaagaaagc
     1021 aattaccttg attctggctg tgctgctggg cggcatggtt ggcgcgggga ttgtgcttgg
     1081 gcgtaacgcg ctgcgtaatt acaacgcgaa ataatattat tgtgcattta agagaaacgg
     1141 gcagggtggt gacaccatgc ccgttttttt tgccggatgc gatgctggcg catcttatcc
     1201 ggcctacgtg tgttgagata atgtgtaggc acgataagct tgcgcatcgg gcaatggctc
     1261 cgggtgtgac aacaacatca cacctgctca gcacgcaact cactgatgcc gtttacgcag
     1321 gttctcaatt
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human chromosome 4 sequence-tagged site STS4-448
Date: 21 Aug 92 10:10:33 GMT
Distribution: bionet
Checksum: 34374     3

LOCUS       HUM4STS448    717 bp ds-DNA             PRI       21-AUG-1992
DEFINITION  Human chromosome 4 sequence-tagged site STS4-448
ACCESSION   L00869
KEYWORDS    STS; human chromosome 4; sequence tagged site.
SOURCE      Homo sapiens, clone p4-291 from a chromosome 4 plasmid library;
            vector pBluescript II KS + (Stratagene)
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 717)
  AUTHORS   Goold,R.D., diSibio,G., Dugaiczyk,A., Smith,K.A., Xu,H.,
            Magrane,G., Lang,D.B., Cox,D.R., Masters,S.B. and Myers,R.M.
  TITLE     Sequence-Tagged Sites for Human Chromosome 4
  JOURNAL   Unpublished (1992) see COMMENT for author address
  STANDARD  full automatic
COMMENT     PCR components: 25 ng of human genomic DNA, 10 pmol of each
            oligonucleotide, 200 micro-M dNTPs, 0.25 U Taq  polymerase (Cetus)
            in 10 micro-l of 50 mM KCl-20 mM  Tris-HCl, pH 8.3 (at room temp),
            2.5 mM MgCl-2.  Initial denaturation at 94degC for 1.5 min, then 30
            cycles of  94degC for 15 sec, 62degC for 23 sec, and 72degC for 30
            sec, followed by a final extension at 72degC for 3.5 min, using a
            Perkin-Elmer 9600 thermocycler. PCR-amplified product size 262 bp.
            Sequence submitted by:
            Human Genome Mapping Center
            Box 0925
            University of California San Francisco
            San Francisco, CA 94143-0925  USA
            Phone: (415) 502-1612   Fax: (415) 476-8391
            e-mail: hgmcprobes@cgl.ucsf.edu
FEATURES             Location/Qualifiers
     misc_feature    286..310
                     /label=Primer1
     misc_feature    complement(523..547)
                     /label=Primer2
BASE COUNT      185 a    120 c    108 g    288 t     16 others
ORIGIN
        1 gatcatatag tagtgtgttt catattgtcc cttctgtctt ctgattcatg aacatagaat
       61 acctttccat ttatttagga ttaacaatta acaattgtta atcctaaata acaatttagg
      121 attgttctct tctgatattg ttttcattac cttagttatc catattctat aatcatccaa
      181 tatattgtta tcattgcttt aagcaaacag atattaactc aattaagaaa aataaaaaga
      241 gatttttact ttactttaaa tcattctttc tttgatattc tttttcatgg aaattcagga
      301 ttctgactta tcccattctc cttcctcctg aaaaacttta agtgtttcct gcangggagg
      361 nctgttgcca ataaatatcc tcagtttggg cntnagnatg acattattta tcattcattt
      421 ctgaagtata atatgtagac atgaaattct agattggtag gagttatttt tcaatacttt
      481 anaganntgt ctctattatc ttcttgtttc catnatttca gtaaagaatt ggctgtcatt
      541 cttatcctaa gggntctgta agtgcctctc atccagcagc ttccttccag acnttctant
      601 tgtctttttt tcccccccac agtttnagaa caatgttttt atgtggtgtg tgtgtgtgtg
      661 tgtgtgtgtg tgtgtgtgtg gtaatcttta ccanagntgg tgntgtctga acatcct
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Lentinellus omphalodes ribosomal RNA (1LR3), large subunit,
Date: 19 Aug 92 10:02:40 GMT
Distribution: bionet
Checksum: 05986     2

LOCUS       LNTRRLSE      207 bp ss-rRNA            RNA       19-AUG-1992
DEFINITION  Lentinellus omphalodes ribosomal RNA (1LR3), large subunit,
            partial.
ACCESSION   M98577
KEYWORDS    ribosomal RNA.
SOURCE      Lentinellus omphalodes (library: Duke Herbarium DSH 89-28) cDNA to
            rRNA.
  ORGANISM  Lentinellus omphalodes
            Eukaryota; Fungi; Eumycota; Basidiomycotina; Hymenomycetes;
            Agaricales; Tricholomataceae.
REFERENCE   1  (bases 1 to 207)
  AUTHORS   Hibbett,D.S. and Vilgalys,R.
  TITLE     Phylogenetic relationships of the basidiomycete genus Lentinus
            inferred from molecular and morphological characters
  JOURNAL   Unpublished (1992)
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     rRNA            <1..>207
                     /note="Aligns to region between positions 371 and 597 in
                     large-subunit nuclear rRNA partial sequence of
                     Saccharomycescerevisiae."
                     /product="large subunit ribosomal RNA"
                     /citation=[1]
BASE COUNT       45 a     47 c     68 g     47 t
ORIGIN
        1 agagagttaa acagtacgtg aaattgttga agggaaacgc ttgaagtcag tcgtccgccg
       61 agactcagcc ttcttctggc ttggtgtact tctcggtgga cgggccagca tcaattttga
      121 ccgccggaca agggcggggg gaaggtggca cttcgggtgt ttatagcccc tcgtcgcata
      181 cggcggtcgg gattgaggaa ctcagca
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human branched chain alpha-keto acid dehydrogenase (BCKDH) E1-beta
Date: 20 Aug 92 10:01:59 GMT
Distribution: bionet
Checksum: 47590     3

LOCUS       HUMBCKD02     108 bp ds-DNA             PRI       20-AUG-1992
DEFINITION  Human branched chain alpha-keto acid dehydrogenase (BCKDH) E1-beta
            subunit, exon 2.
ACCESSION   D90383
KEYWORDS    branched chain alpha-keto acid dehydrogenase;
            maple syrup urine disease.
SEGMENT     2 of 10
SOURCE      Homo sapiens adult peripheral blood DNA.
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 16 to 93)
  AUTHORS   Matsuda,I., Asaka,J., Akaboshi,I., Endo,F., Mitsubuchi,H. and
            Nobukuni,Y.
  TITLE     Maple syrup urine disease: Complete primary structure of the
            E-1-beta subunit of human branched chain alpha-ketoacid
            dehydrogenase complex deduced from the nucleotide sequence and a
            gene analysis of patients with this disease
  JOURNAL   J. Clin. Invest. 86, 242-247 (1990)
  STANDARD  simple staff_review
REFERENCE   2  (bases 1 to 108)
  AUTHORS   Mitsubuchi,H., Nobukuni,Y., Endo,F. and Matsuda,I.
  TITLE     Structural organization and chromosomal localization of the gene
            for the E-1-beta subunit of human branched chain alpha-keto acid
            dehydrogenase
  JOURNAL   J. Biol. Chem. 266, 14686-14691 (1991)
  STANDARD  full staff_review
COMMENT     These data kindly submitted in computer readable form by: Hiroshi
            Mitsubuchi Department of Pediatrics Kumamoto University Medical
            School 1-1-1 Honjo, Kumamoto 860 Japan Phone:  096-344-2111 x5654
            Fax:    096-366-3471
FEATURES             Location/Qualifiers
     intron          <1..15
                     /number=1b
                     /gene="BCKDHB"
     exon            16..93
                     /number=2
                     /note="E-1-beta subunit"
                     /gene="BCKDHB"
     intron          94..>108
                     /number=2a
BASE COUNT       32 a     23 c     20 g     33 t
ORIGIN      chromosome 6p22-p21
        1 ttttgatttt cacagggcaa actcagaaaa tgaatctttt ccagtctgta acaagtgcct
       61 tggataactc attggccaaa gatcctactg caggtaaccc tgatatgt
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human chromosome 4 sequence-tagged site STS4-142
Date: 21 Aug 92 10:06:56 GMT
Distribution: bionet
Checksum: 45087     3

LOCUS       HUM4STS142    415 bp ds-DNA             PRI       21-AUG-1992
DEFINITION  Human chromosome 4 sequence-tagged site STS4-142
ACCESSION   M95274
KEYWORDS    STS; human chromosome 4; sequence tagged site.
SOURCE      Homo sapiens, clone C4-2 from Los Alamos National Laboratory
            chromosome 4 cosmid library, plate 4-1-176R, pos 4B; vector
            SuperCos 1 (Stratagene)
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Goold,R.D., diSibio,G., Dugaiczyk,A., Smith,K.A., Xu,H.,
            Magrane,G., Lang,D.B., Cox,D.R., Masters,S.B. and Myers,R.M.
  TITLE     Sequence-Tagged Sites for Human Chromosome 4
  JOURNAL   Unpublished (1992) see COMMENT for author address
  STANDARD  full automatic
COMMENT     PCR components: 25 ng of human genomic DNA, 10 pmol of each
            oligonucleotide, 200 micro-M dNTPs, 0.25 U Taq  polymerase (Cetus)
            in 10 micro-l of 50 mM KCl-20 mM  Tris-HCl, pH 8.3 (at room temp),
            2.5 mM MgCl-2.  Initial denaturation at 94degC for 1.5 min, then 30
            cycles of  94degC for 15 sec, 62degC for 23 sec, and 72degC for 30
            sec, followed by a final extension at 72degC for 3.5 min, using a
            Perkin-Elmer 9600 thermocycler. PCR-amplified product size 186 bp.
            Sequence submitted by:
            Human Genome Mapping Center
            Box 0925
            University of California San Francisco
            San Francisco, CA 94143-0925  USA
            Phone: (415) 502-1612   Fax: (415) 476-8391
            e-mail: hgmcprobes@cgl.ucsf.edu
FEATURES             Location/Qualifiers
     misc_feature    38..62
                     /label=Primer1
     misc_feature    complement(199..223)
                     /label=Primer2
BASE COUNT      121 a     77 c     52 g    145 t     20 others
ORIGIN
        1 tngatcagac ctatttcagt ttaactataa ataaaaactc cttgaatata tcactactct
       61 catgttcaac gtgcatctgt catccagtga ttaatctttg gatgactttt tngngtgcta
      121 aagttacttt attagaaagt ccagcaaaga gaaatacagt tttggtactg gaaaaatttc
      181 tttttcatca tagttcatca catctgactt agacaatcac atttcttgca agtctgttnt
      241 ttcctctaca tagaactgtc tccacttaag aagatttann aatgcctact ttgtgcataa
      301 tactataaaa gctaaatcan attatncccn cttnttatat nggnatgtct caacttgant
      361 cacttncgna ntattactct cgctattgnt tcattcannc aaacgagatt gnttg
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: S.lividus gene for 16S rRNA (partial)
Date: 22 Aug 92 10:01:48 GMT
Distribution: bionet
Checksum: 55414     2

LOCUS       SYO16SRRN2    249 bp ds-DNA             BCT       22-AUG-1992
DEFINITION  S.lividus gene for 16S rRNA (partial)
ACCESSION   X67092
KEYWORDS    16S rRNA; rRNA.
SOURCE      Synechococcus lividus DNA.
  ORGANISM  Synechococcus lividus
            Prokaryotae; Gracilicutes; Oxyphotobacteria; Cyanobacteria;
            Chroococcales.
REFERENCE   1  (bases 1 to 249)
  AUTHORS   Ward,D.M., Weller,R. and Bateson,M.M.
  TITLE     16S rRNA sequences reveal uncultured inhabitants of a well-studied
            thermal community
  JOURNAL   FEMS Microbiol. Rev. 75, 105-116 (1990)
  STANDARD  full automatic
COMMENT     *source: strain=y-7c7b-S; *source: isolate=Clearwater Spring,
            Yellowstone National Park, USA;
            From EMBL    entry SL16SRRN2;  dated 08-JUL-1992.
BASE COUNT       43 a     54 c     85 g     34 t     33 others
ORIGIN
        1 tgagggccgn agaggctngc ggaattcccg gtgthggggt naaatccgta gatatcggga
       61 ggaacaccgg tggggaagcc ngcyagctng acggttcctn acgctgaggc acnaaagcgt
      121 ggggagcwaa ccggattaga tacccgggtw gtccacgccg tnaacgatgg gcnctwggnn
      181 tgggggtnan nncctctntg ccgtagctna cgcgtnnagc gcnnngccnn gggagnnngg
      241 csgcaaggc
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Clostridium perfringens rrnA operon containing 16S ribosomal RNA.
Date: 21 Aug 92 10:06:06 GMT
Distribution: bionet
Checksum: 02810     3

LOCUS       CLO16SRRNA    420 bp ds-DNA             BCT       21-AUG-1992
DEFINITION  Clostridium perfringens rrnA operon containing 16S ribosomal RNA.
ACCESSION   M69267
KEYWORDS    16S ribosomal RNA.
SOURCE      Clostridium perfringens (strain CPN50) (library: Plasmid PUC18)
            DNA.
  ORGANISM  Clostridium perfringens
            Prokaryotae; Firmicutes; Endospore-forming rods and cocci;
            Bacillaceae.
REFERENCE   1  (bases 1 to 420)
  AUTHORS   Garnier,T., Canard,B. and Cole,S.T.
  TITLE     cloning, mapping, and molcular characterization of the rRNA operons
            of clostridium
  JOURNAL   J. Bacteriol. 173, 5431-5438 (1991)
  STANDARD  full staff_entry
FEATURES             Location/Qualifiers
     misc_feature    50..71
                     /note="Potential upstream activator"
                     /gene="RRS"
     -10_signal      167..172
                     /note="P1 promoter"
                     /gene="RRS"
     rRNA            180..>420
                     /product="16S ribosomal RNA"
                     /gene="RRS"
                     /evidence=EXPERIMENTAL
     -35_signal      254..259
                     /note="P2 promoter"
                     /gene="RRS"
     -10_signal      277..282
                     /note="P2 promoter"
                     /gene="RRS"
     rRNA            289..>420
                     /product="16S ribosomal RNA"
                     /gene="RRS"
     misc_feature    325
                     /note="Prematuration point"
                     /gene="RRS"
                     /evidence=EXPERIMENTAL
     misc_feature    389..393
                     /gene="RRS"
                     /evidence=EXPERIMENTAL
BASE COUNT      192 a     43 c     60 g    125 t
ORIGIN
        1 atatctaaaa atccttacga atagcaaata aatagtataa atataatata aactttacca
       61 agtgtatcaa taattaaatt tttgagaaaa ataaagaaat taagagaaaa tataatataa
      121 ttttataata tagcatataa actaaatgtt tatgtaacct tatgtgataa gatattactt
      181 gtcgctgacg aacagcaaca aaacaaaaaa caaaacttag caaataagtt aaaaaagttt
      241 tttgaagaaa aatgttgaca aagttcgaaa atggtgttaa actaaagaag tcgcttgagg
      301 gcgacaaaga aatggtcttt gaaaattaaa cagaagatat taacataaac cagcaattct
      361 tttataagta atatgagtat agattaaact tttaaattga gagtttgatc ctggctcagg
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human chromosome 4 sequence-tagged site STS4-413
Date: 21 Aug 92 10:10:04 GMT
Distribution: bionet
Checksum: 27619     3

LOCUS       HUM4STS413    435 bp ds-DNA             PRI       21-AUG-1992
DEFINITION  Human chromosome 4 sequence-tagged site STS4-413
ACCESSION   L00892
KEYWORDS    STS; human chromosome 4; sequence tagged site.
SOURCE      Homo sapiens, clone p4-351 from a chromosome 4 plasmid library;
            vector pBluescript II KS + (Stratagene)
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 435)
  AUTHORS   Goold,R.D., diSibio,G., Dugaiczyk,A., Smith,K.A., Xu,H.,
            Magrane,G., Lang,D.B., Cox,D.R., Masters,S.B. and Myers,R.M.
  TITLE     Sequence-Tagged Sites for Human Chromosome 4
  JOURNAL   Unpublished (1992) see COMMENT for author address
  STANDARD  full automatic
COMMENT     PCR components: 25 ng of human genomic DNA, 10 pmol of each
            oligonucleotide, 200 micro-M dNTPs, 0.25 U Taq  polymerase (Cetus)
            in 10 micro-l of 50 mM KCl-20 mM  Tris-HCl, pH 8.3 (at room temp),
            2.5 mM MgCl-2.  Initial denaturation at 94degC for 1.5 min, then 30
            cycles of  94degC for 15 sec, 62degC for 23 sec, and 72degC for 30
            sec, followed by a final extension at 72degC for 3.5 min, using a
            Perkin-Elmer 9600 thermocycler. PCR-amplified product size 220 bp.
            Sequence submitted by:
            Human Genome Mapping Center
            Box 0925
            University of California San Francisco
            San Francisco, CA 94143-0925  USA
            Phone: (415) 502-1612   Fax: (415) 476-8391
            e-mail: hgmcprobes@cgl.ucsf.edu
FEATURES             Location/Qualifiers
     misc_feature    176..201
                     /label=Primer1
     misc_feature    complement(370..395)
                     /label=Primer2
BASE COUNT      137 a    103 c     79 g    106 t     10 others
ORIGIN
        1 gatcacctag aatttggggg gaagtggggt gattgtaagg gcagtctgct tgttctatca
       61 attgttctgt gaattgcatg atagatggag aaccagtgga ggactagtga aaccctcccc
      121 tcagtactga agacagcaag ccacaaagaa cattttaaaa gcttccagag ataaaaagca
      181 gatagtatac acaagaacaa gaattaagct agccttaaaa ctgtcatgaa aatttgnggg
      241 gtctgatgaa aatgttctca agccctcaag acccccttac tctctntntc tctctctctc
      301 tctctctctc tctctcacac acacacacac acacacacac acacacacaa acacacacac
      361 acaatgnaca ttctcttgga agatgtattc ttcaganagg gnnacaccaa tnttcgagnt
      421 gcttcnagta cgtat
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human chromosome 4 sequence-tagged site STS4-155
Date: 21 Aug 92 10:07:04 GMT
Distribution: bionet
Checksum: 08955     3

LOCUS       HUM4STS155    427 bp ds-DNA             PRI       21-AUG-1992
DEFINITION  Human chromosome 4 sequence-tagged site STS4-155
ACCESSION   M95273
KEYWORDS    STS; human chromosome 4; sequence tagged site.
SOURCE      Homo sapiens, clone C4-183 from Los Alamos National Laboratory
            chromosome 4 cosmid library, plate 4-1-2R, pos9C; vector SuperCos 1
            (Stratagene)
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 427)
  AUTHORS   Goold,R.D., diSibio,G., Dugaiczyk,A., Smith,K.A., Xu,H.,
            Magrane,G., Lang,D.B., Cox,D.R., Masters,S.B. and Myers,R.M.
  TITLE     Sequence-Tagged Sites for Human Chromosome 4
  JOURNAL   Unpublished (1992) see COMMENT for author address
  STANDARD  full automatic
COMMENT     PCR components: 25 ng of human genomic DNA, 10 pmol of each
            oligonucleotide, 200 micro-M dNTPs, 0.25 U Taq  polymerase (Cetus)
            in 10 micro-l of 50 mM KCl-20 mM  Tris-HCl, pH 8.3 (at room temp),
            2.5 mM MgCl-2.  Initial denaturation at 94degC for 1.5 min, then 30
            cycles of  94degC for 15 sec, 62degC for 23 sec, and 72degC for 30
            sec, followed by a final extension at 72degC for 3.5 min, using a
            Perkin-Elmer 9600 thermocycler. PCR-amplified product size 292 bp.
            Sequence submitted by:
            Human Genome Mapping Center
            Box 0925
            University of California San Francisco
            San Francisco, CA 94143-0925  USA
            Phone: (415) 502-1612   Fax: (415) 476-8391
            e-mail: hgmcprobes@cgl.ucsf.edu
FEATURES             Location/Qualifiers
     misc_feature    36..61
                     /label=Primer1
     misc_feature    complement(304..327)
                     /label=Primer2
BASE COUNT      131 a     75 c     96 g    115 t     10 others
ORIGIN
        1 naaacattgc aaaaggtgtt tctagttcta cttaccatat taacaagaca aggtacttaa
       61 catgattcaa gttcaaagat gtttctgttc ttataacaaa cctggtttta aaaantgcct
      121 tggtgattct actggcaatg tctgttaaac acatgggttg catcctccct aaagtttcca
      181 tcaaagaaat cttttataac ccagcttcca gtgatgtagt aagcgatacc caccaataag
      241 gcagtgacan cagaaaagag caagtgtggt gggattatgg agtgggtaat tccacaaata
      301 cangtagggc atttactgtg tactaggcac tgcctgacgt gctgcgaatg nggcagcgaa
      361 cangatgtgt gaagatcttg agctcgtagg gtgatactca gtggntanga gggatgaaaa
      421 acangtn
//