From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: P.sativum dhn-cog gene encoding dehydrin-cognate.
Date: 15 Aug 92 10:02:50 GMT
Distribution: bionet
Checksum: 36127     5

LOCUS       PEANCOGG     1035 bp ss-RNA             PLN       15-AUG-1992
DEFINITION  P.sativum dhn-cog gene encoding dehydrin-cognate.
ACCESSION   Z14145
KEYWORDS    dehydrin; dhn-cog gene.
SOURCE      Pisum sativum RNA.
  ORGANISM  Pisum sativum
            Eukaryota; Plantae; Embryobionta; Magnoliophyta; Magnoliopsida;
            Rosidae; Fabales; Fabaceae.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Robertson,M. and Chandler,P.M.
  TITLE     Pea dehydrin cognate : isolation, characterisation and expression
  JOURNAL   Unpublished (1992)
  STANDARD  full staff_review
REFERENCE   2  (bases 1 to 1035)
  AUTHORS   Robertson,M.
  JOURNAL   Unpublished (1992)
  STANDARD  full staff_review
COMMENT     *source: strain=cv. Greenfeast; *source: stage=immature cotyledons;
            *source: tissue=cotyledons; *source: clone_library=cDNA, labmda
            ZAP; *source: clone=pPsB61; *source: isolate=selection 086;
            *source: is_macronuclear=Y;
            From EMBL    entry DHNCOGG;  dated 03-AUG-1992.
FEATURES             Location/Qualifiers
     CDS             162..812
                     /standard_name="DHN-cog"
                     /note="The deduced aminoacid sequence contains two
                     lysine-rich boxes,whichare characteristic unique to
                     dehydrins. Howeverthe gene in not induced by dehydration."
                     /product="dehydrin-cognate"
                     /gene="dhn-cog"
                     /codon_start=1
                     /translation="MAEENQNKYEETTSATNSETEIKDRGVFDFLGGKKKDEEHKPAQ
                     DEAIAHEFGHKVTLYEAPSETKVEEEGEKKHTSLLEKLHRSDSSSSSSSEEEGEDGEK
                     RKKKKEKKKKEDTSVPVEKVEVVEGTTGTEEEKGFLDKIKEKLPGGHKKTEEVTTPPP
                     VVAAHVPTETTATTTIHEGEKKGILEKIKEKLPGYHAKTATDGEDKDHHKDETTSHS"
                     /citation=[1]
                     /citation=[2]
     misc_feature    414..440
                     /note="a stretch of serine residues"
                     /citation=[1]
                     /citation=[2]
     misc_feature    579..602
                     /note="lysine-rich box. The region translatesinto a
                     conserved motif of dehydrin"
                     /evidence=EXPERIMENTAL
                     /citation=[1]
                     /citation=[2]
     misc_feature    720..743
                     /note="lysine-rich box. The region translatesinto a
                     conserved motif of dehydrin"
                     /evidence=EXPERIMENTAL
                     /citation=[1]
                     /citation=[2]
     polyA_site      932
                     /note="polyA site ofanother cDNA clone with identical
                     coding sequence"
                     /evidence=EXPERIMENTAL
                     /citation=[1]
                     /citation=[2]
     polyA_site      991
                     /note="polyA site ofanother cDNA clone with identical
                     coding sequence"
                     /evidence=EXPERIMENTAL
                     /citation=[1]
                     /citation=[2]
BASE COUNT      349 a    158 c    228 g    300 t
ORIGIN
        1 tttttttttt tttttttttt tttttttttt tttaaaacaa caacaacact atcatcatcg
       61 tctcatactt tacctttcat ttcagagtta cagctacata atccatttta gttacagtta
      121 attaatttcc agttcgatcc atcttgttag tagtgatcat catggctgag gagaatcaga
      181 acaagtacga ggagactacc tccgcaacca actctgagac agagatcaag gacagaggtg
      241 tttttgattt tctcggtggt aagaaaaagg atgaagaaca taagcctgct caagatgagg
      301 cgattgccca cgagtttggt cacaaggtga ctttgtatga agcaccgtcg gaaaccaaag
      361 ttgaagagga aggtgaaaag aaacacacca gtctcttgga gaaacttcac cgatctgaca
      421 gctcatcaag ctcctcaagt gaagaggaag gagaagatgg tgagaaaaga aagaagaaga
      481 aggaaaagaa gaagaaagaa gacacttcgg tgccagttga gaaagttgag gttgttgaag
      541 gaacaaccgg tactgaggaa gagaagggtt tccttgacaa aattaaggag aagcttcccg
      601 gaggacacaa gaaaacagag gaagtaacaa ctcctccacc agttgtcgct gctcatgttc
      661 caactgagac aacagctaca acaactattc atgagggaga gaagaaaggt attttggaaa
      721 agatcaaaga gaagcttcct ggttatcatg ctaagactgc tactgatggt gaagacaaag
      781 atcatcacaa ggatgagact acttctcatt gaatatgatc gattggttgt gttggtttgt
      841 ggctttgggg gtgttttgtt taattaatta ctatcattaa ggccttgtgt ggtgatttct
      901 ttcttgaatt aatggtatat gtatttggtt cttaacttgt ctttgtgggt acttttttaa
      961 atacacataa aagaaaaatt tgtttgggtt tattatattt tttagattga aaagaaaaaa
     1021 aaaaaaaaaa aaaaa
//