From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: E.coli (clones pLC26-6, pLC4-14, pLC6-7) complete 2 minute region
Date: 18 Aug 92 10:00:40 GMT
Distribution: bionet
Checksum: 05030    67

LOCUS       ECO2MIN     28277 bp ds-DNA             BCT       18-AUG-1992
DEFINITION  E.coli (clones pLC26-6, pLC4-14, pLC6-7) complete 2 minute region
            (1.77 to 2.38 minutes).
ACCESSION   X55034
KEYWORDS    ddl gene; envA gene; ftsA gene; ftsQ gene; ftsW gene; ftsZ gene;
            ilvH gene; ilvI gene; leuA gene; leuO gene; mraY gene; murC gene;
            murD gene; murE gene; murF gene; murG gene; mutT gene; orfA gene;
            orfB; orfC; orfX; pbpB gene; secA gene; shl gene.
SOURCE      Escherichia coli (sub_strain W3110, strain K-12) DNA.
  ORGANISM  Escherichia coli
            Prokaryotae; Gram-negative facultatively anaerobic rods;
            Enterobacteriaceae.
REFERENCE   1  (bases 1 to 306)
  AUTHORS   Wessler,S.R. and Calvo,J.M.
  TITLE     Control of leu operon expression in Escherichia coli by a
            transcription attenuation mechanism
  JOURNAL   J. Mol. Biol. 149, 579-579 (1981)
  STANDARD  full automatic
REFERENCE   2  (bases 7316 to 10074)
  AUTHORS   Nakamura,M., Maruyama,I.N., Soma,M., Kato,J.I., Suzuki,H. and
            Hirota,Y.
  TITLE     On the process of cellular division in Escherichia coli: Nucleotide
            sequence of the gene for penicillin-binding protein 3
  JOURNAL   Mol. Gen. Genet. 191, 1-9 (1983)
  STANDARD  full automatic
REFERENCE   3  (bases 2101 to 4431)
  AUTHORS   Squires,C.H., DeFelice,M., Devereux,J. and Calvo,J.M.
  TITLE     Molecular structure of ilvIH and its evolutionary relationship to
            ilvG in Escherichia coli
  JOURNAL   Nucleic Acids Res. 11, 5299-5313 (1983)
  STANDARD  full automatic
REFERENCE   4  (bases 19464 to 21952)
  AUTHORS   Robinson,A.C., Kenan,D.J., Hatfull,G.F., Sullivan,N.F.,
            Spiegelberg,R. and Donachie,W.D.
  TITLE     DNA sequence and transcriptional organization of essential cell
            division genes ftsQ and ftsA of Escherichia coli: Evidence for
            overlapping transcriptional units
  JOURNAL   J. Bacteriol. 160, 546-555 (1984)
  STANDARD  full automatic
REFERENCE   5  (bases 21464 to 23333)
  AUTHORS   Yi,Q.M. and Lutkenhaus,J.
  TITLE     The nucleotide sequence of the essential cell-division gene ftsZ of
            Escherichia coli
  JOURNAL   Gene 36, 241-247 (1985)
  STANDARD  full automatic
REFERENCE   6  (bases 1799 to 2187)
  AUTHORS   Haughn,G.W., Squires,C.H., DeFelice,M., Largo,C.T. and Calvo,J.M.
  TITLE     Unusual organization of the ilvIH promoter of Escherichia coli
  JOURNAL   J. Bacteriol. 163, 186-198 (1985)
  STANDARD  full automatic
REFERENCE   7  (bases 268 to 1130)
  AUTHORS   Haughn,G.W., Wessler,S.R., Gemunill,R.M. and Calvo,J.M.
  TITLE     High A+T content conserved in DNA sequences upstream of leuABCD in
            Escherichia coli and Salmonella typhimurium
  JOURNAL   J. Bacteriol. 166, 1113-1117 (1986)
  STANDARD  full automatic
REFERENCE   8  (bases 18619 to 19770)
  AUTHORS   Robinson,A.C., Kenan,D.J., Sweeney,J. and Donachie,W.D.
  TITLE     Further Evidence for Overlapping Transcriptional Units in an
            Escherichia coli Cell Envelope-Cell Division Gene Cluster: DNA
            Sequence and Transcriptional Organization of the ddl ftsQ Region
  JOURNAL   J. Bacteriol. 167, 809-817 (1986)
  STANDARD  full automatic
REFERENCE   9  (bases 22964 to 25011)
  AUTHORS   Beall,B. and Lutkenhaus,J.
  TITLE     Sequence analysis, transcriptional organization and insertional
            mutagenesis of the envA gene of Escherichia coli
  JOURNAL   J. Bacteriol. 169, 5408-5415 (1987)
  STANDARD  full automatic
REFERENCE   10 (bases 27412 to 28277)
  AUTHORS   Akiyama,M., Horiuchi,T. and Sekiguchi,M.
  TITLE     Molecular cloning and nucleotide sequence of the mutT mutator of
            Escherichia coli that causes A:T to C:G transversion
  JOURNAL   Mol. Gen. Genet. 206, 9-16 (1987)
  STANDARD  full automatic
REFERENCE   11 (bases 23989 to 27799)
  AUTHORS   Schmidt,M., Rollo,E., Grodberg,I. and Oliver,D.
  TITLE     Nucleotide sequence of secA gene and secA(ts) mutations preventing
            protein export in Escherichia coli
  JOURNAL   J. Bacteriol. 170, 3404-3414 (1988)
  STANDARD  full automatic
REFERENCE   12 (bases 20513 to 21772)
  AUTHORS   Robinson,A.C., Begg,K.J. and Donachie,W.D.
  TITLE     Mapping and characterization of mutants of the Escherichia coli
            cell division gene, ftsA
  JOURNAL   Mol. Microbiol. 2, 581-588 (1988)
  STANDARD  full automatic
REFERENCE   13 (bases 843 to 1812)
  AUTHORS   Henikoff,S., Haughn,G.W., Calvo,J.M. and Wallance,J.C.
  TITLE     A large family of bacterial activator proteins
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 85, 6602-6606 (1988)
  STANDARD  full automatic
REFERENCE   14 (sites)
  AUTHORS   Tao,J. and Ishiguro,E.E.
  TITLE     Nucleotide sequence of the murE gene of Escherichia coli
  JOURNAL   Can. J. Microbiol. 35, 1051-1054 (1989)
  STANDARD  full staff_review
REFERENCE   15 (bases 14743 to 16239)
  AUTHORS   Ikeda,M., Sato,T., Wachi,M., Jung,H.K., Ishino,F., Kobayashi,Y. and
            Matsuhashi,M.
  TITLE     Structural similarity among Escherichia coli FtsW and RodA proteins
            and Bacillus subtilis SpoVE protein, which function in cell
            division, cell elongation and spore formation, respectively
  JOURNAL   J. Bacteriol. 171, 6375-6378 (1989)
  STANDARD  full automatic
REFERENCE   16 (bases 11142 to 12634)
  AUTHORS   Parquet,C., Fluoret,B., Menguin-Lecreulx,D. and Van Heijenoort,J.
  TITLE     Nucleotide sequence of the murF gene encoding the UDP-MurNac-
            pentapeptide synthetase of Escherichia coli
  JOURNAL   Nucleic Acids Res. 17, 5379-5379 (1989)
  STANDARD  full automatic
REFERENCE   17 (sites)
  AUTHORS   Michaud,C., Parquet,C., Flouret,B., Blanot,D. and Van Heijenoort,J.
  TITLE     Revised interpretation of the sequence containing the murE gene
            encoding the UDP-N-acetylmuramyl-tripeptide synthetase of
            Escherichia coli
  JOURNAL   Biochem. J. 269, 277-280 (1990)
  STANDARD  full staff_review
REFERENCE   18 (bases 4274 to 6093)
  AUTHORS   Leclerc,G., Noel,G. and Drapeau,G.R.
  TITLE     Molecular cloning, nucleotide sequence, and expression of shl, a
            new gene in the 2-minute region of the genetic map of Escherichia
            coli
  JOURNAL   J. Bacteriol. 172, 4696-4700 (1990)
  STANDARD  full automatic
REFERENCE   19 (bases 13392 to 15020)
  AUTHORS   Menguin-Lecreulx,D. and Van Heijenoort,J.
  TITLE     Nucleotide sequence of the murD gene encoding the UDP-MurNac-
            L-Ala-D-gly synthetase of Escherichia coli
  JOURNAL   Nucleic Acids Res. 18, 183-183 (1990)
  STANDARD  full automatic
REFERENCE   20 (bases 12423 to 15030)
  AUTHORS   Ikeda,M., Wachi,M., Ishino,F. and Matsuhashi,M.
  TITLE     Nucleotide sequence involving murD and an open reading frame orf-Y
            spacing murF and ftsW in Escherichia coli
  JOURNAL   Nucleic Acids Res. 18, 1058-1058 (1990)
  STANDARD  full automatic
REFERENCE   21 (bases 16094 to 17806)
  AUTHORS   Menguin-Lecreulx,D., Texier,L. and Van Heijenoort,J.
  TITLE     Nucleotide sequence of the cell-envelope murG gene of Escherichia
            coli
  JOURNAL   Nucleic Acids Res. 18, 2810-2810 (1990)
  STANDARD  full automatic
REFERENCE   22 (bases 6088 to 7587)
  AUTHORS   Gomez,M.J., Fluoret,B., Van Heijenoort,J. and Ayala,J.A.
  TITLE     Nucleotide sequence of the regulatory region of pbpB gene of
            Escherichia coli
  JOURNAL   Nucleic Acids Res. 18, 2813-2813 (1990)
  STANDARD  full automatic
REFERENCE   23 (bases 16094 to 18886)
  AUTHORS   Ikeda,M., Wachi,M., Jung,H.K., Ishino,F. and Matsuhashi,M.
  TITLE     Nucleotide sequence involving murG and murC in the mra gene cluster
            region of Escherichia coli
  JOURNAL   Nucleic Acids Res. 18, 4014-4014 (1990)
  STANDARD  full automatic
REFERENCE   24 (bases 1 to 28277)
  AUTHORS   Ayala,J.A.
  JOURNAL   Unpublished (1991)
  STANDARD  full automatic
REFERENCE   25 (bases 1 to 28277)
  AUTHORS   Ayala,J.A.
  TITLE     Regulation of transcription at the 2-minute region of the genetic
            map of Escherichia coli.
  JOURNAL   Unpublished (1991)
  STANDARD  full automatic
REFERENCE   26 (bases 4274 to 6093)
  AUTHORS   Jahreis,K., Postma,P.W. and Lengeler,J.W.
  TITLE     Nucleotide sequence of the ilv H-fruR gene of Escherichia coli K-12
            and Salmonella typhimurium LT2
  JOURNAL   Unpublished (1991)
  STANDARD  full automatic
COMMENT     *source: strain=K12 W3110; clone=pLC26-6, pLC4-14, pLC6-7 **map:
            map position=1.77 to 2.38 mins This entry comprises a merged
            sequence of 28Kb of which a portion is the submittor's original
            work.
            From EMBL 27   entry EC2MIN;  dated 08-APR-1991.
FEATURES             Location/Qualifiers
     mRNA            complement(<1..269)
                     /gene="leuA"
                     /evidence=EXPERIMENTAL
     CDS             complement(<1..63)
                     /product="alpha-isopropylmalate synthase"
                     /gene="leuA"
                     /codon_start=1
                     /translation="MSQQVIIFDTTLRDGEQALQA"
     CDS             complement(156..242)
                     /note="leu operon leader peptide"
                     /gene="leuA"
                     /codon_start=1
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     CDS             725..1774
                     /product="LeuO protein"
                     /gene="leuO"
                     /codon_start=1
                     /translation="MTHSTAMDSVFIRTRIFMFSEFYSFCFFLFYMHDKSYSSGLFLC
                     IPIRERELSVTVELSMPEVQTDHPETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAA
                     HVLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELP
                     GSGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRY
                     QETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLD
                     RFASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQV
                     LPLPLKQNSRTCYLS"
     promoter        1916..1946
                     /standard_name="P4"
                     /gene="ilvIH"
     mRNA            1928..4378
                     /note="minor alternate mRNA (in vivo)"
                     /gene="ilvIH"
                     /evidence=EXPERIMENTAL
                     /partial
     mRNA            1954..4378
                     /note="minor alternate mRNA (in vitro)"
                     /gene="ilvIH"
                     /evidence=EXPERIMENTAL
                     /partial
     promoter        1957..2001
                     /standard_name="P3"
                     /gene="ilvIH"
     promoter        2039..2067
                     /standard_name="P2"
                     /gene="ilvIH"
     promoter        2097..2125
                     /standard_name="P1"
                     /gene="ilvIH"
     mRNA            2129..4378
                     /note="major alternate mRNA (in vivo)"
                     /gene="ilvIH"
                     /evidence=EXPERIMENTAL
                     /partial
     CDS             2164..3864
                     /product="acetohydroxy acid synthase (AHAS) III"
                     /gene="ilvI"
                     /codon_start=1
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVWPESVSMRSYNPTTTGHKGQIKRALQSVVAVKKPVVYVGGGAI
                     TAGCHQQLKETVEALNLPVVCSLMGLGAFPATHRQVLGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRARQCLKYDTHSEKIKPQAVIETLWRLTKGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGSMGFGLPAALGVKMAFAEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRRG
                     AYGHVGIQISHPHGWKANLARRWNRCAIIAWCLLMLPSMAASTSTRCRFAGAEWMKCG
                     "
     CDS             3887..4378
                     /product="acetohydroxy acid synthase (AHAS) III"
                     /gene="ilvH"
                     /codon_start=1
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSGKLSAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     conflict        replace(4280..4281,"ga")
                     /citation=[18]
                     /citation=[26]
     -35_signal      4338..4346
                     /gene="shl"
     conflict        replace(4362
                     /citation=[3]
     -10_signal      4363..4368
                     /gene="shl"
     RBS             4377..4380
                     /note="leader peptide RBS"
                     /gene="shl"
     conflict        replace(4388..4394
                     /citation=[3]
     CDS             4390..4476
                     /note="shl operon leader peptide"
                     /gene="shl"
                     /codon_start=1
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     RBS             4547..4550
                     /gene="shl"
     CDS             4558..5562
                     /product="Shl protein"
                     /gene="shl"
                     /codon_start=1
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     CDS             6164..6622
                     /note="OrfC"
                     /codon_start=1
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIYHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
     misc_feature    6390..6411
                     /note="SOS box I (ORF B)"
     misc_feature    6453..6476
                     /note="SOS box II (ORF B)"
     -35_signal      6496..6501
                     /note="ORF B"
     -10_signal      6521..6527
                     /note="ORF"
     mRNA            6540..7565
                     /note="ORF B"
                     /evidence=EXPERIMENTAL
                     /partial
     RBS             6606..6609
                     /note="ORF B"
     CDS             6624..7565
                     /note="OrfB protein"
                     /codon_start=1
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     mRNA            7219..7928
                     /note="ORF A alternate mRNA (in vivo)"
                     /evidence=EXPERIMENTAL
                     /partial
     -35_signal      7507..7512
                     /note="ORF A"
     -10_signal      7529..7534
                     /note="ORF A"
     misc_feature    7534..7554
                     /note="SOS box III (ORF A)"
     mRNA            7542..7928
                     /note="ORF A alternate mRNA (in vivo)"
                     /evidence=EXPERIMENTAL
                     /partial
     RBS             7551..7554
                     /note="ORF A"
     CDS             7562..7927
                     /note="ORF A"
                     /codon_start=1
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     RBS             7927..7930
                     /gene="pbpB"
     CDS             7943..9709
                     /product="Pbp3 protein"
                     /gene="pbp3"
                     /codon_start=1
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     RBS             9686..9689
                     /gene="murE"
     CDS             9696..11183
                     /product="UDP-MurNac-tripeptide synthetase"
                     /gene="murE"
                     /codon_start=1
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGEISAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     TEVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     conflict        replace(9876,"g")
                     /citation=[17]
     RBS             11168..11175
                     /gene="murF"
     sig_peptide     11180..11239
                     /gene="murF"
                     /codon_start=1
     CDS             11180..12538
                     /product="UDP-MurNac-pentapeptide synthetase"
                     /gene="murF"
                     /codon_start=1
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGAAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPERALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSVGK
                     QSHAISTASGVGEHFADKTALITRLKLLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     mat_peptide     11240..12535
                     /product="UDP-MurNac-pentapeptide synthetase"
                     /gene="murF"
                     /codon_start=1
     RBS             12519..12523
                     /gene="mraY"
     CDS             12532..13614
                     /product="MraY protein"
                     /gene="mraY"
                     /codon_start=1
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     conflict        replace(12568
                     /citation=[16]
     conflict        replace(12570
                     /citation=[16]
     conflict        replace(12611..12612,"ct")
                     /citation=[16]
     conflict        replace(12625..12626,"ca")
                     /citation=[16]
     conflict        replace(13533
                     /citation=[19]
     -35_signal      13541..13546
                     /gene="murD"
     -10_signal      13562..13567
                     /gene="murD"
     RBS             13602..13606
                     /gene="murD"
     CDS             13617..14933
                     /product="UDP-MurNac-L-Ala-D-Glu"
                     /gene="murD"
                     /codon_start=1
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDDECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     HVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     conflict        replace(13698..13699,"gc")
                     /citation=[19]
     conflict        replace(14136..14138,"acg")
                     /citation=[19]
     conflict        replace(14442..14445,"cgcg")
                     /citation=[19]
     CDS             14933..16177
                     /product="FtsW protein"
                     /gene="ftsW"
                     /codon_start=1
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     RBS             16184..16188
                     /gene="murG"
     CDS             16198..17241
                     /product="MurG protein"
                     /gene="murG"
                     /codon_start=1
                     /translation="MVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADRMEADLVPK
                     HGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGMGGYVSGPG
                     GLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGNPVRTDVLA
                     LPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQSGKGSQQSV
                     EQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPALFVPFQHK
                     DRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERARAASIPDAT
                     ERVANEVSRVARA"
     RBS             17274..17279
                     /gene="murC"
     CDS             17295..18770
                     /EC_number="6.3.2.8"
                     /product="UDP-N-acetylmuramate-alanine ligase"
                     /gene="murC"
                     /codon_start=1
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPARVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     conflict        replace(18660
                     /citation=[8]
                     /citation=[4]
     RBS             18751..18754
                     /gene="ddl"
     CDS             18763..19683
                     /product="D-Ala:D-Ala ligase"
                     /gene="ddl"
                     /codon_start=1
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     -35_signal      19509..19514
                     /gene="ftsQ"
     -10_signal      19536..19541
                     /gene="ftsQ"
     CDS             19685..20515
                     /product="FtsQ protein"
                     /gene="ftsQ"
                     /codon_start=1
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPERTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     -35_signal      20070..20075
                     /gene="ftsA"
     -10_signal      20092..20097
                     /gene="ftsA"
     -35_signal      20420..20425
                     /gene="ftsA"
     -10_signal      20445..20450
                     /gene="ftsA"
     CDS             20512..21774
                     /product="FtsA protein"
                     /gene="ftsA"
                     /codon_start=1
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     -35_signal      21187..21192
                     /gene="ftsZ"
     -10_signal      21211..21216
                     /gene="ftsZ"
     conflict        replace(21526..21527,"cg")
                     /citation=[4]
     -35_signal      21542..21547
                     /gene="ftsZ"
     -10_signal      21565..21570
                     /gene="ftsZ"
     promoter        21725..21808
                     /note="proximal ftsZ"
                     /gene="ftsZ"
     -35_signal      21753..21758
                     /gene="ftsZ"
     -10_signal      21776..21781
                     /gene="ftsZ"
     RBS             21823..21828
                     /gene="ftsZ"
     CDS             21835..22986
                     /product="FtsZ protein"
                     /gene="ftsZ"
                     /codon_start=1
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVNSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGHAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     conflict        replace(21930
                     /citation=[21]
     -35_signal      23001..23006
                     /gene="envA"
     conflict        replace(23012..23013,"gg")
                     /citation=[5]
     -10_signal      23024..23029
                     /gene="envA"
     CDS             23087..24004
                     /product="EnvA protein"
                     /gene="envA"
                     /codon_start=1
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     conflict        replace(23318,"c")
                     /citation=[5]
     CDS             24304..24747
                     /note="ORF X"
                     /codon_start=1
                     /translation="MVAASLGLPALSNAAEPNAPAKATTRNHEPSAKVNFGQLALLEA
                     NTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTLPVAEESLPLQAQHLALLDTLSAL
                     LTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQGIRAGPQRLT"
     CDS             24809..27514
                     /product="SecA protein"
                     /gene="secA"
                     /codon_start=1
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRDGIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     -35_signal      27460..27465
                     /gene="mutT"
     -10_signal      27485..27490
                     /gene="mutT"
     RBS             27562..27565
                     /gene="mutT"
     RBS             27567..27570
                     /note="mutT RBS2"
                     /gene="mutT"
     mat_peptide     27574..27960
                     /product="MutT protein"
                     /gene="mutT"
                     /codon_start=1
     CDS             27574..27963
                     /product="MutT protein"
                     /gene="mutT"
                     /codon_start=1
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVERWEGEPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
BASE COUNT     6714 a   6725 c   7993 g   6845 t
ORIGIN
        1 tgcctgtaac gcctgttcac cgtcgcgcaa tgtggtatcg aaaataatga cttgctggct
       61 catggtttgg gtccttgtct cttttagagc gcctcgcttc gggcataaaa aaacccgcgc
      121 aatggcgcgg gttttttgtt tgactgcgtg ctggcttaat gctggatgcc gctcactcgt
      181 ctaccgcgca aagaagatgc gtttagtagt agtagaccga taaagcgaac gatgtgagtc
      241 attaaatcag ctccagatga atgcgatatg cttttagagt tactggatac aaaaacggat
      301 gtcaaccctg acgcaataaa aacgtcccgc cagcgtgagt tctgcatccg taaaattagc
      361 taattgtgct gcggtggtta aagtaagcga tattaatttc tgcttaacta ccgacgcttt
      421 tcatcggttg acatatttca gcataaattt ttgcatctaa tcaacgagga aaaaggggac
      481 aaaatgcacg cgttgcaaaa cctatcctga tgatttgtat tgaattatat gttttgcgat
      541 tttttttgat attgatttgg tgaatattat tgatcaatta atgttaagaa ttaatgcatt
      601 aaatatataa attaattatt aaataagcac atttaatcca ttttgtagat gattgagtat
      661 tcgcggtagt tatgattaga ttgttttcgc aacaaaaaca ttatggatta ttatgctgtg
      721 gtaaatgact cattccacgg caatggattc tgtttttatc agaacccgta tctttatgtt
      781 ttccgaattt tactcatttt gctttttctt attttatatg catgataaat catattcttc
      841 aggattattt ctctgcattc caataaggga aagggagtta agtgtgacag tggagttaag
      901 tatgccagag gtacaaacag atcatccaga gacggcggag ttaagcaaac cacagctacg
      961 catggtcgat ctcaacttat taaccgtttt cgatgccgtg atgcaggagc aaaacattac
     1021 tcgtgccgct catgttctgg gaatgtcgca acctgcggtc agtaacgctg ttgcacgcct
     1081 gaaggtgatg tttaatgacg agctttttgt tcgttatggc cgtggtattc aaccgactgc
     1141 tcgcgcattt caactttttg gttcagttcg tcaggcattg caactagtac aaaatgaatt
     1201 gcctggttca ggttttgaac ccgcgagcag tgaacgtgta tttcatcttt gtgtttgcag
     1261 cccgttagac agcattctga cctcgcagat ttataatcac attgagcaga ttgcgccaaa
     1321 tatacatgtt atgttcaagt cttcattaaa tcagaacact gaacatcagc tgcgttatca
     1381 ggaaacggag tttgtgatta gttatgaaga cttccatcgt cctgaattta ccagcgtacc
     1441 attatttaaa gatgaaatgg tgctggtagc cagcaaaaat catccaacaa ttaagggccc
     1501 gttactgaaa catgatgttt ataacgaaca acatgcggcg gtttcgctcg atcgtttcgc
     1561 gtcatttagt caaccttggt atgacacggt agataagcaa gccagtatcg cgtatcaggg
     1621 catggcaatg atgagcgtac ttagcgtggt gtcgcaaacg catttggtcg ctattgcgcc
     1681 gcgttggctg gctgaagagt tcgctgaatc cttagaatta caggtattac cgctgccgtt
     1741 aaaacaaaac agcagaacct gttatctctc ctagcatgaa gctgccgggc gcgataaagg
     1801 ccatcagtgg atggaagagc aattagtctc aatttgcaaa cgctaactga ttgcagaata
     1861 ggtcagacat gaatgtctgg tttattctgc attttttatt gaatgtagaa ttttattctg
     1921 aatgtgtggg ctctctattt taggattaat taaaaaaata gagaaattgc tgtaagttgt
     1981 gggattcagc cgatttatta tcaatttaat cctctgtaat ggaggatttt atcgtttctt
     2041 ttcacctttc ctcctgttta ttcttattac cccgtgttta tgtctctggc tgccaattgc
     2101 ttaagcaaga tcggacggtt aatgtgtttt acacattttt tccgtcaaac agtgaggcag
     2161 gccatggaga tgttgtctgg agccgagatg gtcgtccgat cgcttatcga tcagggcgtt
     2221 aaacaagtat tcggttatcc cggaggcgca gtccttgata tttatgatgc attgcatacc
     2281 gtgggtggta ttgatcatgt attagttcgt catgagcagg cggcggtgca tatggccgat
     2341 ggcttggcgc gcgcgaccgg ggaagtcggc gtcgtgctgg taacgtcggg tccaggggcg
     2401 accaatgcga ttactggcat cgccaccgct tatatggatt ccattccatt agttgtcctt
     2461 tccgggcagg tagcgacctc gttgataggt tacgatgcct ttcaggagtg cgacatggtg
     2521 gggatttcgc gacccgtggt taaacacagt tttctggtta agcaaacgga agacattccg
     2581 caggtgctga aaaaggcttt ctggctggcg gcaagcggtc gcccaggacc agtagtcgtt
     2641 gatttaccga aagatattct taatccggcg aacaaattac cctatgtctg gccggagtcg
     2701 gtcagtatgc gttcttacaa tcccactact accggacata aagggcaaat taagcgtgct
     2761 ctgcaaagcg tggtagcggt aaaaaaaccg gttgtctacg taggcggtgg ggcaatcacg
     2821 gcgggctgcc atcagcagtt gaaagaaacg gtggaggcgt tgaatctgcc cgttgtttgc
     2881 tcattgatgg ggctgggggc gtttccggca acgcatcgtc aggtactggg tatgctggga
     2941 atgcacggta cctacgaagc caatatgacg atgcataacg cggatgtgat tttcgccgtc
     3001 ggggtacgat ttgatgaccg aacgacgaac aatctggcaa agtactgccc aaatgccact
     3061 gttctgcata tcgatattga tcctacttcc atttctaaaa ccgtgactgc ggatatcccg
     3121 attgtggggg atgctcgcca agtcctcgaa caaatgcttg aactcttgtc gcaagaatcc
     3181 gcccatcaac cactggatga gatccgcgac tggtggcagc aaattgaaca gtggcgcgct
     3241 cgtcagtgcc tgaaatatga cactcacagt gaaaagatta aaccgcaggc ggtgatcgag
     3301 actctttggc ggttgacgaa gggagacgct tacgtgacgt ccgatgtcgg gcagcaccag
     3361 atgtttgctg cactttatta tccattcgac aaaccgcgtc gctggatcaa ttccggtggc
     3421 ctcggcagca tgggttttgg tttacctgcg gcactgggcg tcaaaatggc gttcgcagaa
     3481 gaaaccgtgg tttgcgtcac tggcgacggc agtattcaga tgaacatcca ggaactgtct
     3541 accgcgttgc aatacgagtt gcccgtactg gtggtgaatc tcaataaccg ctatctgggg
     3601 atggtgaagc agtggcagga catgatctat tccggccgtc attcacaatc ttatatgcaa
     3661 tcgctacccg atttcgtccg tcgcggagcc tatgggcatg tcgggatcca gatttctcat
     3721 ccgcatggct ggaaagcaaa cttagcgagg cgctggaaca ggtgcgcaat aatcgcctgg
     3781 tgtttgttga tgttaccgtc gatggcagcg agcacgtcta cccgatgcag attcgcgggg
     3841 gcggaatgga tgaaatgtgg ttaagcaaaa cggagagaac ctgattatgc gccggatatt
     3901 atcagtctta ctcgaaaatg aatcaggcgc gttatcccgc gtgattggcc ttttttccca
     3961 gcgtggctac aacattgaaa gcctgaccgt tgcgccaacc gacgatccga cattatcgcg
     4021 tatgaccatc cagaccgtgg gcgatgaaaa agtacttgag cagatcgaaa agcaattaca
     4081 caaactggtc gatgtcttgc gcgtgagtga gttggggcag ggcgcgcatg ttgagcggga
     4141 aatcatgctg gtgaaaattc aggccagcgg ttacgggcgt gacgaagtga aacgtaatac
     4201 ggaaatattc cgtgggcaaa ttatcgatgt cacaccctcg ctttataccg ttcaattagc
     4261 aggcaccagc ggtaagctta gtgcattttt agcatcgatt cgcgatgtgg cgaaaattgt
     4321 ggaggttgct cgctctggtg tggtcggact ttcgcgcggc gataaaataa tgcgttgaga
     4381 atgatctcaa tgcgcaattt acagcccaac atgtcacgtt gggctttttt tgcgaaatca
     4441 gtgggaacct ggaataaaag cagttgccgc agttaatttt ctgcgcttag atgttaatga
     4501 atttaaccca taccagtaca atggctatgg tttttacatt ttacgcaagg ggcaattgtg
     4561 aaactggatg aaatcgctcg gctggcggga gtgtcgcgga ccactgcaag ctatgttatt
     4621 aacggcaaag cgaagcaata ccgtgtgagc gacaaaaccg ttgaaaaagt catggctgtg
     4681 gtgcgtgagc acaattacca cccgaacgcc gtggcagctg ggcttcgtgc tggacgcaca
     4741 cgttctattg gtcttgtgat ccccgatctg gagaacacca gctatacccg catcgctaac
     4801 tatcttgaac gccaggcgcg gcaacggggt tatcaactgc tgattgcctg ctcagaagat
     4861 cagccagaca acgaaatgcg gtgcattgag caccttttac agcgtcaggt tgatgccatt
     4921 attgtttcga cgtcgttgcc tcctgagcat cctttttatc aacgctgggc taacgacccg
     4981 ttcccgattg tcgcgctgga ccgcgccctc gatcgtgaac acttcaccag cgtggttggt
     5041 gccgatcagg atgatgccga aatgctggcg gaagagttac gtaagtttcc cgccgagacg
     5101 gtgctttatc ttggtgcgct accggagctt tctgtcagct tcctgcgtga acaaggtttc
     5161 cgtactgcct ggaaagatga tccgcgcgaa gtgcatttcc tgtatgccaa cagctatgag
     5221 cgggaggcgg ctgcccagtt attcgaaaaa tggctggaaa cgcatccgat gccgcaggcg
     5281 ctgttcacaa cgtcgtttgc gttgttgcaa ggagtgatgg atgtcacgct gcgtcgcgac
     5341 ggcaaactgc cttctgacct ggcaattgcc acctttggcg ataacgaact gctcgacttc
     5401 ttacagtgtc cggtgctggc agtggctcaa cgtcaccgcg atgtcgcaga gcgtgtgctg
     5461 gagattgtcc tggcaagcct ggacgaaccg cgtaagccaa aacctggttt aacgcgcatt
     5521 aaacgtaatc tctatcgccg cggcgtgctc agccgtagct aagccgcgaa caaaaatacg
     5581 cgccaggtga atttccctct ggcgcgtaga gtacgggact ggacatcaat atgcttaaag
     5641 taaataagac tattcctgac tattattgat aaatgctttt aaacccgccc gttaattaac
     5701 tcaccagctg aaattcacaa taattaagtg atatcgacag cgcgtttttg cattattttg
     5761 ttacatgcgg cgatgaattg ccgatttaac aaacactttt ctttgctttt gcgcaaaccc
     5821 gctggcatca agcgccacac agacgtaaca aggactgtta accggggaag atatgtccta
     5881 aaatgccgct cgcgtcgcaa actgacactt tatatttgct gtggaaaata gtgagtcatt
     5941 ttaaaacggt gatgacgatg agggattttt tcttacagct attcataacg ttaatttgct
     6001 tcgcacgttg gacgtaaaat aaacaacgct gatattagcc gtaaacatcg ggttttttac
     6061 ctcggtatgc cttgtgactg gcttgacaag cttttcctca gctccgtaaa ctcctttcag
     6121 tgggaaattg tggggcaaag tgggaataag gggtgaggct ggcatgttcc ggggagcaac
     6181 gttagtcaat ctcgacagca aagggcgctt atcagtgcct acccgttatc gggaacagct
     6241 gcttgagaac gctgccggtc aaatggtttg caccattgac atttatcacc cgtgcctgct
     6301 gctttacccc ctgcctgaat gggaaattat cgagcaaaaa ttatcgcgtc tgtcgagcat
     6361 gaacccggtt gagcgccgtg tgcagcgcct actgttaggt catgccagcg aatgtcagat
     6421 ggatggcgca ggtcgattgt taatcgcgcc agtactgcgg caacatgccg ggctgacaaa
     6481 agaagtgatg ctggttggac agttcaacaa gtttgagctg tgggatgaaa caacctggca
     6541 tcaacaggtc aaggaagata tcgacgcaga gcagttggct accggagact tatcggagcg
     6601 actgcaggac ttgtctctat aaaatgatgg aaaactataa acatactacg gtgctgctgg
     6661 atgaagccgt taatggcctc aatatccgtc ctgatggcat ctacattgat gggacttttg
     6721 gtcgcggtgg tcactcacgt ctgatcctct cgcagcttgg cgaagagggg cgtttgctgg
     6781 cgatcgatcg cgacccgcag gctatcgccg ttgcgaagac tattgatgat ccgcgcttct
     6841 ccatcatcca cggacctttc tccgcgctgg gcgaatacgt tgccgagcgc gatcttatcg
     6901 gcaagatcga cggcattctc ctcgatcttg gcgtctcttc accgcaactt gatgatgctg
     6961 aacgtggctt ttcctttatg cgcgatggtc cgctggacat gcgtatggac ccaacccgtg
     7021 ggcagtcagc cgctgaatgg ctacaaaccg cagaagaagc cgatatcgcc tgggtattga
     7081 aaacctatgg tgaagagcgt tttgccaaac gcattgcccg cgccattgtc gagcgtaacc
     7141 gcgaacagcc gatgacccgc accaaagaac tggcggaagt cgtggctgct gcaacgccgg
     7201 tgaaagataa gtttaaacat cccgcgaccc gtaccttcca ggcggtgcgc atttgggtaa
     7261 acagtgaact ggaggagata gagcaggcgc taaaaagctc gctcaacgtg ctggccccgg
     7321 gtgggcggct ttcgatcatc agcttccact cgctggaaga ccgtattgtg aaacgtttta
     7381 tgcgtgaaaa cagccgcggt ccgcaagttc cggcagggtt accgatgact gaagagcagc
     7441 tcaaaaaact gggtggccgt cagctgcgag cactaggcaa gttaatgccg ggcgaagaag
     7501 aggtggctga gaaccctcgt gcccgtagtt cagttctgcg tattgcagag aggacgaatg
     7561 catgatcagc agagtgacag aagctctaag caaagttaaa ggatcgatgg gaagccacga
     7621 gcgccatgca ttgcctggtg ttatcggtga cgatcttttg cgatttggga agctgccact
     7681 ctgcctgttc atttgcatta ttttgacggc ggtgactgtg gtaaccacgg cgcaccatac
     7741 ccgtttactg accgctcagc gcgaacaact ggtgctggag cgagatgctt tagacattga
     7801 atggcgcaac ctgatccttg aagagaatgc gctcggcgac catagccggg tggaaaggat
     7861 cgccacggaa aagctgcaaa tgcagcatgt tgatccgtca caagaaaata tcgtagtgca
     7921 aaaataagga taaacgcgac gcatgaaagc agcggcgaaa acgcagaaac caaaacgtca
     7981 ggaagaacat gccaacttta tcagttggcg ttttgcgttg ttatgcggct gtattctcct
     8041 ggcgctggct tttctgctcg gacgcgtagc gtggttacaa gttatctccc cggatatgct
     8101 ggtgaaagag ggcgacatgc gttctcttcg cgttcagcaa gtttccacct cccgcggcat
     8161 gattactgac cgttctggtc gcccgttagc ggtgagcgtg ccggtaaaag cgatttgggc
     8221 tgacccgaaa gaagtgcatg acgctggcgg tatcagcgtc ggtgaccgct ggaaggcgct
     8281 ggctaacgcg ctcaatattc cgctggatca gctttcagcc cgcattaacg ccaacccgaa
     8341 agggcgcttt atttatctgg cgcgtcaggt gaaccctgac atggcggact acatcaaaaa
     8401 actgaaactg ccggggattc atctgcgtga agagtctcgc cgttactatc cgtccggcga
     8461 agtgactgct cacctcatcg gctttactaa cgtcgatagt caagggattg agggcgttga
     8521 gaagagtttc gataaatggc ttaccgggca gccgggtgag cgcattgtgc gtaaagaccg
     8581 ctatggtcgc gtaattgaag atatttcttc tactgacagc caggcagcgc acaacctggc
     8641 gctgagtatt gatgaacgcc tgcaggcgct ggtttatcgc gaactgaaca acgcggtggc
     8701 ctttaacaag gctgaatctg gtagcgccgt gctggtggat gtcaacaccg gtgaagtgct
     8761 ggcgatggct aacagcccgt catacaaccc taacaatctg agcggcacgc cgaaagaggc
     8821 gatgcgtaac cgtaccatca ccgacgtgtt tgaaccgggc tcaacggtta aaccgatggt
     8881 ggtaatgacc gcgttgcaac gtggcgtggt gcgggaaaac tcggtactca ataccattcc
     8941 ttatcgaatt aacggccacg aaatcaaaga cgtggcacgc tacagcgaat taaccctgac
     9001 cggggtatta cagaagtcga gtaacgtcgg tgtttccaag ctggcgttag cgatgccgtc
     9061 ctcagcgtta gtagatactt actcacgttt tggactggga aaagcgacca atttggggtt
     9121 ggtcggagaa cgcagtggct tatatcctca aaaacaacgg tggtctgaca tagagagggc
     9181 caccttctct ttcggctacg ggctaatggt aacaccatta cagttagcgc gagtctacgc
     9241 aactatcggc agctacggca tttatcgccc actgtcgatt accaaagttg accccccggt
     9301 tcccggtgaa cgtgtcttcc cggaatccat tgtccgcact gtggtgcata tgatggaaag
     9361 cgtggcgcta ccaggcggcg gcggcgtgaa ggcggcgatt aaaggctatc gtatcgccat
     9421 taaaaccggt accgcgaaaa aggtcgggcc ggacggtcgc tacatcaata aatatattgc
     9481 ttataccgca ggcgttgcgc ctgcgagtca gccgcgcttc gcgctggttg ttgttatcaa
     9541 cgatccgcag gcgggtaaat actacggcgg cgccgtttcc gcgccggtct ttggtgccat
     9601 catgggcggc gtattgcgta ccatgaacat cgagccggat gcgctgacaa cgggcgataa
     9661 aaatgaattt gtgattaatc aaggcgaggg gacaggtggc agatcgtaat ttgcgcgacc
     9721 ttcttgctcc gtgggtgcca gacgcacctt cgcgagcact gcgagagatg acactcgaca
     9781 gccgtgtggc tgcggcgggc gatctctttg tagctgtagt aggtcatcag gcggacgggc
     9841 gtcgatatat cccgcaggcg atagcgcaag gtgtggctgc cattattgca gaggcgaaag
     9901 atgaggcgac cgatggtgaa atccgtgaaa tgcacggcgt accggtcatc tatctcagcc
     9961 agctcaacga gcgtttatct gcactggcgg gccgctttta ccatgaaccc tctgacaatt
    10021 tacgtctcgt gggcgtaacg ggcaccaacg gcaaaaccac gactacccag ctgttggcgc
    10081 agtggagcca actgcttggc gaaatcagcg cggtaatggg caccgttggt aacggcctgc
    10141 tggggaaagt gatcccgaca gaaaatacaa ccggttcggc agtcgatgtt cagcatgagc
    10201 tggcggggct ggtggatcag ggcgcgacgt tttgcgcaat ggaagtttcc tcccacgggc
    10261 tggtacagca ccgtgtggcg gcattgaaat ttgcggcgtc ggtctttacc aacttaagcc
    10321 gcgatcacct tgattatcat ggtgatatgg aacactacga agccgcgaaa tggctgcttt
    10381 attctgagca tcattgcggt caggcgatta ttaacgccga cgatgaagtg ggccgccgct
    10441 ggctggcaaa actgccggac gcggttgcgg tatcaatgga agatcatatt aatccgaact
    10501 gtcacggacg ctggttgaaa gcgaccgaag tgaactatca cgacagcggt gcgacgattc
    10561 gctttagctc aagttggggc gatggcgaaa ttgaaagcca tctgatgggc gcttttaacg
    10621 tcagcaacct gctgctcgcg ctggcgacac tgttggcact cggctatcca ctggctgatc
    10681 tgctgaaaac cgccgcgcgt ctgcaaccgg tttgcggacg tatggaagtg ttcactgcgc
    10741 caggcaaacc gacggtggtg gtggattacg cgcatacgcc ggatgcactg gaaaaagcct
    10801 tacaggcggc gcgtctgcac tgtgcgggca agctgtggtg tgtctttggc tgtggtggcg
    10861 atcgcgataa aggtaagcgt ccactgatgg gcgcaattgc cgaagagttt gctgacgtgg
    10921 cggtggtgac ggacgataac ccgcgtaccg aagaaccgcg tgccatcatc aacgatattc
    10981 tggcgggaat gttagatgcc ggacatgcca aagtgatgga aggccgtgct gaagcggtga
    11041 cttgcgccgt tatgcaggct aaagagaatg atgtggtact ggtcgcgggc aaaggccatg
    11101 aagattacca gattgttggc aatcagcgtc tggactactc cgatcgcgtc acggtggcgc
    11161 gtctgctggg ggtgattgca tgattagcgt aacccttagc caacttaccg acattctcaa
    11221 cggtgaactg caaggtgcag atatcaccct tgatgctgta accactgata cccgaaaact
    11281 gacgccgggc tgcctgtttg ttgccctgaa aggcgaacgt tttgatgccc acgattttgc
    11341 cgaccaggcg aaagctggcg cggcaggcgc actactggtt agccgtccgc tggacatcga
    11401 cctgccgcag ttaatcgtca aggatacgcg tctggcgttt ggtgaactgg ctgcatgggt
    11461 tcgccagcaa gttccggcgc gcgtggttgc tctgacgggg tcctccggca aaacctccgt
    11521 taaagagatg acggcggcga ttttaagcca gtgcggcaac acgctttata cggcaggcaa
    11581 tctcaacaac gacatcggtg taccgatgac gctgttgcgc ttaacgccgg aatacgatta
    11641 cgcagttatt gaacttggcg cgaaccatca gggcgaaata gcctggactg tgagtctgac
    11701 tcgcccggaa cgtgcgctgg tcaacaacct ggcagcggcg catctggaag gttttggctc
    11761 gcttgcgggt gtcgcgaaag cgaaaggtga aatctttagc ggcctgccgg aaaacggtat
    11821 cgccattatg aacgccgaca acaacgactg gctgaactgg cagagcgtaa ttggctcacg
    11881 caaagtgtgg cgtttctcac ccaatgccgc caacagcgat ttcaccgcca ccaatatcca
    11941 tgtgacctcg cacggtacgg aatttaccct acaaacccca accggtagcg tcgatgttct
    12001 gctgccgttg ccggggcgtc acaatattgc gaatgcgctg gcagccgctg cgctctccat
    12061 gtccgtgggc gcaacgcttg atgctatcaa agcggggctg gcaaatctga aagctgttcc
    12121 aggccgtctg ttccccatcc aactggcaga aaaccagttg ctgctcgacg actcctacaa
    12181 cgccaatgtc ggttcaatga ctgcagcagt ccaggtactg gctgaaatgc cgggctaccg
    12241 cgtgctggtg gtgggcgata tggcggaact gggcgctgaa agcgaagcct gccatgtaca
    12301 ggtgggcgag gcggcaaaag ctgctggtat tgaccgcgtg ttaagcgtgg gtaaacaaag
    12361 ccatgctatc agcaccgcca gcggcgttgg cgaacatttt gctgataaaa ctgcgttaat
    12421 tacgcgtctt aaattactga ttgctgagca acaggtaatt acgattttag ttaagggttc
    12481 acgtagtgcc gccatggaag aggtagtacg cgctttacag gagaatggga catgttagtt
    12541 tggctggccg aacatttggt caaatattat tccggcttta acgtcttttc ctatctgacg
    12601 tttcgcgcca tcgtcagcct gctgaccgcg ctgttcatct cattgtggat gggcccgcgt
    12661 atgattgctc atttgcaaaa actttccttt ggtcaggtgg tgcgtaacga cggtcctgaa
    12721 tcacacttca gcaagcgcgg tacgccgacc atgggcggga ttatgatcct gacggcgatt
    12781 gtgatctccg tactgctgtg ggcttacccg tccaatccgt acgtctggtg cgtgttggtg
    12841 gtgctggtag gttacggtgt tattggcttt gttgatgatt atcgcaaagt ggtgcgtaaa
    12901 gacaccaaag ggttgatcgc tcgttggaag tatttctgga tgtcggtcat tgcgctgggt
    12961 gtcgccttcg ccctgtacct tgccggcaaa gacacgcccg caacgcagct ggtggtccca
    13021 ttctttaaag atgtgatgcc gcagctgggg ctgttctaca ttctgctggc ttacttcgtc
    13081 attgtgggta ctggcaacgc ggtaaacctg accgatggtc tcgacggcct ggcaattatg
    13141 ccgaccgtat ttgtcgccgg tggttttgcg ctggtggcgt gggcgaccgg caatatgaac
    13201 tttgccagct acttgcatat accgtatctg cgacacgccg gggaactggt tattgtctgt
    13261 accgcgatag tcggggcagg actgggcttc ctgtggttta acacctatcc ggcgcaggtc
    13321 tttatgggcg atgtaggttc gctggcgtta ggtggtgcgt taggcattat cgccgtactg
    13381 ctacgtcagg aattcctgct ggtgattatg gggggcgtgt tcgtggtaga aacgctttct
    13441 gtcatcctgc aggtcggctc ctttaaactg cgcggacaac gtattttccg catggcaccg
    13501 attcatcacc actatgaact gaaaggctgg ccggaaccgc gcgtcattgt gcgtttctgg
    13561 attatttcgc tgatgctggt tctgattggt ctggcaacgc tgaaggtacg ttaatcatgg
    13621 ctgattatca gggtaaaaat gtcgtcatta tcggcctggg cctcaccggg ctttcctgcg
    13681 tggacttttt cctcgctcgc ggtgtgacgc cgcgcgttat ggatacgcgt atgacaccgc
    13741 ctggcctgga taaattaccc gaagccgtag aacgccacac gggcagtctg aatgatgaat
    13801 ggctgatggc ggcagatctg attgtcgcca gtcccggtat tgcactggcg catccatcct
    13861 taagcgctgc cgctgatgcc ggaatcgaaa tcgttggcga tatcgagctg ttctgtcgcg
    13921 aagcacaagc accgattgtg gcgattaccg gttctaacgg caaaagcacg gtcaccacgc
    13981 tagtgggtga aatggcgaaa gcggcggggg ttaacgttgg tgtgggtggc aatattggcc
    14041 tgcctgcgtt gatgctactg gatgatgagt gtgaactgta cgtgctggaa ctgtcgagct
    14101 tccagctgga aaccacctcc agcttacagg cggtagcagc gaccattctg aacgtgactg
    14161 aagatcatat ggatcgctat ccgtttggtt tacaacagta tcgtgcagca aaactgcgca
    14221 tttacgaaaa cgcgaaagtt tgcgtggtta atgctgatga tgccttaaca atgccgattc
    14281 gcggtgcgga tgaacgctgc gtcagctttg gcgtcaacat gggtgactat cacctgaatc
    14341 atcagcaggg cgaaacctgg ctgcgggtta aaggcgagaa agtgctgaat gtgaaagaga
    14401 tgaaactttc cgggcagcat aactacacca atgcgctggc ggcgctggcg ctggcagatg
    14461 ctgcagggtt accgcgtgcc agcagcctga aagcgttaac cacattcact ggtctgccgc
    14521 atcgctttga agttgtgctg gagcataacg gcgtacgttg gattaacgat tcgaaagcga
    14581 ccaacgtcgg cagtacggaa gcggcgctga atggcctgca cgtagacggc acactgcatt
    14641 tgttgctggg tggcgatggt aaatcggcgg actttagccc actggcgcgt tacctgaatg
    14701 gcgataacgt acgtctgtat tgtttcggtc gtgacggcgc gcagctggcg gcgctacgcc
    14761 cggaagtggc agaacaaacc gaaactatgg aacaggcgat gcgcttgctg gctccgcgtg
    14821 ttcagccggg cgatatggtt ctgctctccc cagcctgtgc cagccttgat cagttcaaga
    14881 actttgaaca acgaggcaat gagtttgccc gtctggcgaa ggagttaggt tgatgcgttt
    14941 atctctccct cgcctgaaaa tgccgcgcct gccaggattc agtatcctgg tctggatctc
    15001 cacggcgcta aagggctggg tgatgggctc gcgggaaaaa gataccgaca gcctgatcat
    15061 gtacgatcgc accttactgt ggctgacctt cggcctcgcg gcgattggct ttatcatggt
    15121 gacctcggcg tcaatgccca tagggcaacg cttaaccaac gatccgttct tcttcgcgaa
    15181 gcgtgatggt gtctatctga ttttggcgtt tattctggcg atcattacgc tgcgtctgcc
    15241 gatggagttc tggcaacgct acagtgccac gatgctgctc ggatctatca tcctgctgat
    15301 gatcgtcctg gtagtgggta gctcggttaa aggggcatcg cgttggatcg atctcggttt
    15361 gctgcgtatc cagcctgcgg agctgacaaa actgtcgctg ttttgctata tcgccaacta
    15421 tctggtgcgt aaaggcgacg aagtacgtaa taacctgcgc ggcttcctga aaccgatggg
    15481 cgtgattctg gtgttggcag tgttactgct ggcacagcca gaccttggta cggtggtggt
    15541 gttgtttgtg actacgctgg cgatgttgtt cctggcggga gcgaaattgt ggcagttcat
    15601 tgccattatc ggtatgggca tttcagcggt tgtgttgctg atactcgccg aaccgtaccg
    15661 tatccgccgt gttaccgcat tctggaaccc gtgggaagat ccctttggca gcggctatca
    15721 gttaacgcaa tcgctgatgg cgtttggtcg cggcgaactt tgggggcaag gtttaggtaa
    15781 ctcggtacaa aaactggagt atctgccgga agcgcacact gactttattt tcgccattat
    15841 cggcgaagaa ctggggtatg tcggtgtggt gctggcactt ttaatggtat tcttcgtcgc
    15901 ttttcgcgcg atgtcgattg gccgtaaagc attagaaatt gaccaccgtt tttccggttt
    15961 tctcgcctgt tctattggca tctggtttag cttccaggcg ctggttaacg taggcgcggc
    16021 ggcggggatg ttaccgacca aaggtctgac attgccgctg atcagttacg gtggttcgag
    16081 cttactgatt atgtcgacag ccatcatgat gctgttgcgt attgattatg aaacgcgtct
    16141 ggagaaagcg caggcgtttg tacgaggttc acgatgagtg gtcaaggaaa gcgattaatg
    16201 gtgatggcag gcggaaccgg tggacatgta ttcccgggac tggcggttgc gcaccatcta
    16261 atggctcagg gttggcaagt tcgctggctg gggactgccg accgtatgga agcggactta
    16321 gtgccaaaac atggcatcga aattgatttc attcgtatct ctggtctgcg tggaaaaggt
    16381 ataaaagcac tgatagctgc cccgctgcgt atcttcaacg cctggcgtca ggcgcgggcg
    16441 attatgaaag cgtacaaacc tgacgtggtg ctcggtatgg gaggctacgt gtcaggtcca
    16501 ggtggtctgg ccgcgtggtc gttaggcatt ccggttgtac ttcatgaaca aaacggtatt
    16561 gcgggcttaa ccaataaatg gctggcgaag attgccacca aagtgatgca ggcgtttcca
    16621 ggtgctttcc ctaatgcgga agtagtgggt aacccggtgc gtaccgatgt gttggcgctg
    16681 ccgttgccgc agcaacgttt ggctggacgt gaaggtccgg ttcgtgtgct ggtagtgggt
    16741 ggttctcagg gcgcacgcat tcttaaccag acaatgccgc aggttgctgc gaaactgggt
    16801 gattcagtca ctatctggca tcagagcggc aaaggttcgc aacaatccgt tgaacaggcg
    16861 tatgccgaag cggggcaacc gcagcataaa gtgacggaat ttattgatga tatggcggcg
    16921 gcgtatgcgt gggcggatgt cgtcgtttgc cgctccggtg cgttaacggt gagtgaaatc
    16981 gccgcggcag gactaccggc gttgtttgtg ccgtttcaac ataaagaccg ccagcaatac
    17041 tggaatgcgc taccgctgga aaaagcgggc gcagccaaaa ttatcgagca gccacagctt
    17101 agcgtggatg ctgtcgccaa caccctggcc gggtggtcgc gagaaacctt attaaccatg
    17161 gcagaacgcg cccgcgctgc atccattccg gatgccaccg agcgagtggc aaatgaagtg
    17221 agccgggttg cccgggcgta attgtagcga tgccttttgc atcgtatgaa tttaagaagt
    17281 taatggcgta aagaatgaat acacaacaat tggcaaaact gcgttccatc gtgcccgaaa
    17341 tgcgtcgcgt tcggcacata cattttgtcg gcattggtgg tgccggtatg ggcggtattg
    17401 ccgaagttct ggccaatgaa ggttatcaga tcagtggttc cgatttagcg ccaaatccgg
    17461 tcacgcagca gttaatgaat ctgggtgcga cgatttattt caaccatcgc ccggaaaacg
    17521 tacgtgatgc cagcgtggtc gttgtttcca gcgcgatttc tgccgataac ccggaaattg
    17581 tcgccgctca tgaagcgcgt attccggtga tccgtcgtgc cgaaatgctg gctgagttaa
    17641 tgcgttttcg tcatggcatc gccattgccg gaacgcacgg caaaacgaca accaccgcga
    17701 tggtttccag catctacgca gaagcggggc tcgacccaac cttcgttaac ggcgggctgg
    17761 taaaagcggc gggggttcat gcgcgtttgg ggcatggtcg gtacctgatt gccgaagcag
    17821 atgagagtga tgcatcgttc ctgcatctgc aaccgatggt ggcgattgtc accaatatcg
    17881 aagccgacca catggatacc taccagggcg actttgagaa tttaaaacag acttttatta
    17941 attttctgca caacctgccg ttttacggtc gtgcggtgat gtgtgttgat gatccggtga
    18001 tccgcgaatt gttaccgcga gtggggcgtc agaccacgac ttacggcttc agcgaagatg
    18061 ccgacgtgcg tgtagaagat tatcagcaga ttggcccgca ggggcacttt acgctgctgc
    18121 gccaggacaa agagccgatg cgcgtcaccc tgaatgcgcc aggtcgtcat aacgcgctga
    18181 acgccgcagc tgcggttgcg gttgctacgg aagagggcat tgacgacgag gctattttgc
    18241 gggcgcttga aagcttccag gggactggtc gccgttttga tttcctcggt gaattcccgc
    18301 tggagccagt gaatggtaaa agcggtacgg caatgctggt cgatgactac ggccaccacc
    18361 cgacggaagt ggacgccacc attaaagcgg cgcgcgcagg ctggccggat aaaaacctgg
    18421 taatgctgtt tcagccgcac cgttttaccc gtacgcgcga cctgtatgat gatttcgcca
    18481 atgtgctgac gcaggttgat accctgttga tgctggaagt gtatccggct ggcgaagcgc
    18541 caattccggg agcggacagc cgttcgctgt gtcgcacaat tcgtggacgt gggaaaattg
    18601 atcccattct ggtgccggat ccggcgcggg tagccgagat gctggcaccg gtattaaccg
    18661 gtaacgacct gattctcgtt cagggggctg gtaatattgg aaaaattgcc cgttctttag
    18721 ctgaaatcaa actgaagccg caaactccgg aggaagaaca acatgactga taaaatcgcg
    18781 gtcctgttgg gtgggacctc cgctgagcgg gaagtttctc tgaattctgg cgcagcggtg
    18841 ttagccggac tgcgtgaagg cggtattgac gcgtatcctg tcgacccgaa agaagtcgac
    18901 gtgacgcaac tgaagtcgat gggctttcag aaagtgttta tcgcgctaca cggtcgcggc
    18961 ggtgaagatg gtacgctgca ggggatgctc gagctgatgg gcttgcctta taccggaagc
    19021 ggagtgatgg catctgcgct ttcaatggat aaactacgca gcaaacttct atggcaaggt
    19081 gccggtttac cggtcgcgcc gtgggtagcg ttaacccgcg cagagtttga aaaaggcctg
    19141 agcgataagc agttagcaga aatttctgct ctgggtttgc cggttatcgt taagccgagc
    19201 cgcgaaggtt ccagtgtggg aatgtcaaaa gtagtagcag aaaatgctct acaagatgca
    19261 ttaagattgg catttcagca cgatgaagaa gtattgattg aaaaatggct aagtgggccg
    19321 gagttcacgg ttgcgatact cggtgaagaa attttaccgt caatacgtat tcaaccgtcc
    19381 ggaaccttct atgattatga ggcgaagtat ctctctgatg agacacagta tttctgcccc
    19441 gcaggtctgg aagcgtcaca agaggccaat ttgcaggcat tagtgctgaa agcatggacg
    19501 acgttaggtt gcaaaggatg gggacgtatt gacgttatgc tggacagcga tggacagttt
    19561 tatctgctgg aagccaatac ctcaccgggt atgaccagcc acagcctggt gccgatggcg
    19621 gcacgtcagg caggtatgag cttctcgcag ttggtagtac gaattctgga actggcggac
    19681 taatatgtcg caggctgctc tgaacacgcg aaacagcgaa gaagaggttt cttctcgccg
    19741 caataatgga acgcgtctgg cggggatcct tttcctgctg accgttttaa cgacagtgtt
    19801 ggtgagcggc tgggtcgtgt tgggctggat ggaagatgcg caacgcctgc cgctctcaaa
    19861 gctggtgttg accggtgaac gccattacac acgtaatgac gatatccggc agtcgatcct
    19921 ggcattgggt gagccgggta cctttatgac ccaggatgtc aacatcatcc agacgcaaat
    19981 agaacaacgc ctgccgtgga ttaagcaggt gagcgtcaga aagcagtggc ctgatgaatt
    20041 gaagattcat ctggttgaat atgtgccgat tgcgcggtgg aatgatcaac atatggtaga
    20101 cgcggaagga aataccttca gcgtgccgcc agaacgcacc agcaagcagg tgcttccaat
    20161 gctgtatggc ccggaaggca gcgccaatga agtgttgcag ggctatcgcg aaatggggca
    20221 gatgctggca aaggacagat ttactctgaa ggaagcggcg atgaccgcgc ggcgttcctg
    20281 gcagttgacg ctgaataacg atattaagct caatcttggc cggggcgata cgatgaaacg
    20341 tttggctcgc tttgtagaac tttatccggt tttacagcag caggcgcaaa ccgatggcaa
    20401 acggattagc tacgttgatt tgcgttatga ctctggagcg gcagtaggct gggcgccctt
    20461 gccgccagag gaatctactc agcaacaaaa tcaggcacag gcagaacaac aatgatcaag
    20521 gcgacggaca gaaaactggt agtaggactg gagattggta ccgcgaaggt tgccgcttta
    20581 gtaggggaag ttctgcccga cggtatggtc aatatcattg gcgtgggcag ctgcccgtcg
    20641 cgtggtatgg ataaaggcgg ggtgaacgac ctcgaatccg tggtcaagtg cgtacaacgc
    20701 gccattgacc aggcagaatt gatggcagat tgtcagatct cttcggtata tctggcgctt
    20761 tctggtaagc acatcagctg ccagaatgaa attggtatgg tgcctatttc tgaagaagaa
    20821 gtgacgcaag aagatgtgga aaacgtcgtc cataccgcga aatcggtgcg tgtgcgcgat
    20881 gagcatcgtg tgctgcatgt gatcccgcaa gagtatgcga ttgactatca ggaagggatc
    20941 aagaatccgg taggactttc gggcgtgcgg atgcaggcaa aagtgcacct gatcacatgt
    21001 cacaacgata tggcgaaaaa catcgtcaaa gcggttgaac gttgtgggct gaaagttgac
    21061 caactgatat ttgccggact ggcatcaagt tattcggtat tgacggaaga tgaacgtgaa
    21121 ctgggtgtct gcgtcgtcga tatcggtggt ggtacaatgg atatcgccgt ttataccggt
    21181 ggggcattgc gccacactaa ggtaattcct tatgctggca atgtcgtgac cagtgatatc
    21241 gcttacgcct ttggcacgcc gccaagcgac gccgaagcga ttaaagttcg ccacggttgt
    21301 gcgctgggtt ccatcgttgg aaaagatgag agcgtggaag tgccgagcgt aggtggtcgt
    21361 ccgccacgga gtctgcaacg tcagacactg gcagaggtga tcgagccgcg ctataccgag
    21421 ctgctcaacc tggtcaacga agagatattg cagttgcagg aaaagcttcg ccaacaaggg
    21481 gttaaacatc acctggcggc aggcattgta ttaaccggtg gcgcagcgca gatcgaaggt
    21541 cttgcagcct gtgctcagcg cgtgtttcat acgcaagtgc gtatcggcgc gccgctgaac
    21601 attaccggtt taacggatta tgctcaggag ccgtattatt cgacggcggt gggattgctt
    21661 cactatggga aagagtcaca tcttaacggt gaagctgaag tagaaaaacg tgttacagca
    21721 tcagttggct cgtggatcaa gcgactcaat agttggctgc gaaaagagtt ttaattttta
    21781 tgaggccgac gatgattacg gcctcaggcg acaggcacaa atcggagaga aactatgttt
    21841 gaaccaatgg aacttaccaa tgacgcggtg attaaagtca tcggcgtcgg cggcggcggc
    21901 ggtaatgctg ttgaacacat ggtgcgcgag cgcattgaag gtgttgaatt cttcgcggta
    21961 aataccgatg cacaagcgct gcgtaaaaca gcggttggac agacgattca aatcggtagc
    22021 ggtatcacca aaggactggg cgctggcgct aatccagaag ttggccgcaa tgcggctgat
    22081 gaggatcgcg atgcattgcg tgcggcgctg gaaggtgcag acatggtctt tattgctgcg
    22141 ggtatgggtg gtggtaccgg tacaggtgcg gcaccagtcg tcgctgaagt ggcaaaagat
    22201 ttgggtatcc tgaccgttgc tgtcgtcact aagcctttca actttgaagg caagaagcgt
    22261 atggcattcg cggagcaggg gatcactgaa ctgtccaagc atgtgaactc tctgatcact
    22321 atcccgaacg acaaactgct gaaagttctg ggccgcggta tctccctgct ggatgcgttt
    22381 ggcgcagcga acgatgtact gaaaggcgct gtgcaaggta tcgctgaact gattactcgt
    22441 ccgggtttga tgaacgtgga ctttgcagac gtacgcaccg taatgtctga gatgggccac
    22501 gcaatgatgg gttctggcgt ggcgagcggt gaagaccgtg cggaagaagc tgctgaaatg
    22561 gctatctctt ctccgctgct ggaagatatc gacctgtctg gcgcgcgcgg cgtgctggtt
    22621 aacatcacgg cgggcttcga cctgcgtctg gatgagttcg aaacggtagg taacaccatc
    22681 cgtgcatttg cttccgacaa cgcgactgtg gttatcggta cttctcttga cccggatatg
    22741 aatgacgagc tgcgcgtaac cgttgttgcg acaggtatcg gcatggacaa acgtcctgaa
    22801 atcactctgg tgaccaataa gcaggttcag cagccagtga tggatcgcta ccagcagcat
    22861 gggatggctc cgctgaccca agagcagaag ccggttgcta aagtcgtgaa tgacaatgcg
    22921 ccgcaaactg cgaaagagcc ggattatctg gatatcccag cattcctgcg taagcaagct
    22981 gattaagaat tgactggaat ttgggtttcg attctctttg tgctaaactg gcccgccgaa
    23041 tgtatagtac acttcggttg gataggtaat ttggcgagat aatacgatga tcaaacaaag
    23101 gacacttaaa cgtatcgttc aggcgacggg tgtcggttta cataccggca agaaagtcac
    23161 cctgacgtta cgccctgcgc cggccaacac cggggtcatc tatcgtcgca ccgacttgaa
    23221 tccaccggta gatttcccgg ccgatgccaa atctgtgcgt gataccatgc tctgtacgtg
    23281 tctggtcaac gagcatgatg tacggatttc aaccgtagag cacctcaatg ctgctctcgc
    23341 gggcttgggc atcgataaca ttgttatcga agttaacgcg ccggaaatcc cgatcatgga
    23401 cggcagcgcc gctccgtttg tatacctgct gcttgacgcc ggtatcgacg agttgaactg
    23461 cgccaaaaaa tttgttcgca tcaaagagac tgttcgtgtc gaagatggcg ataagtgggc
    23521 tgaatttaag ccgtacaatg gtttttcgct ggatttcacc atcgatttta accatccggc
    23581 tattgattcc agcaaccagc gctatgcgat gaacttctcc gctgatgcgt ttatgcgcca
    23641 gatcagccgt gcgcgtacgt tcggtttcat gcgtgatatc gaatatctgc agtcccgtgg
    23701 tttgtgcctg ggcggcagct tcgattgtgc catcgttgtt gacgattatc gcgtactgaa
    23761 cgaagacggc ctgcgttttg aagacgaatt tgtgcgtcac aaaatgctcg atgcgatcgg
    23821 tgacttgttc atgtgtggtc acaatattat tggtgcattt accgcttata aatccggtca
    23881 tgcactgaat aacaaactgc tgcaggctgt cctggcgaaa caggaagcct gggaatatgt
    23941 gaccttccag gacgacgcag aactgccgtt ggccttcaaa gcgccttcag ctgtactggc
    24001 ataacgacat ttatactgtc gtataaaatt cgactggcaa atctggcact ctctccggcc
    24061 aggtgaacca gtcgtttttt tttgaatttt ataagagcta taaaaaacgg tgcgaacgct
    24121 gttttcttaa gcacttttcc gcacaactta tcttcattcg tgctgtggac tgcaggcttt
    24181 aatgataaga tttgtgcgct aaatacgttt gaatatgatc gggatggcaa taacgtgagt
    24241 ggaatactga cgcgctggcg acagtttggt aaacgctact tctggccgca tctcttatta
    24301 gggatggttg cggcgagttt aggtttgcct gcgctcagca acgccgccga accaaacgcg
    24361 cccgcaaaag cgacaacccg caaccacgag ccttcagcca aagttaactt tggtcaattg
    24421 gccttgctgg aagcgaacac acgccgcccg aattcgaact attccgttga ttactggcat
    24481 caacatgcca ttcgcacggt aatccgtcat ctttctttcg caatggcacc gcaaacactg
    24541 cccgttgctg aagaatcttt gcctcttcag gcgcaacatc ttgcattact ggatacgctc
    24601 agcgcgctgc tgacccagga aggcacgccg tctgaaaagg gttatcgcat tgattatgcg
    24661 cattttaccc cacaagcaaa attcagcacg cccgtctgga taagccaggc gcaaggcatc
    24721 cgtgctggcc ctcaacgcct cacctaacaa caataaacct ttacttcatt ttattaactc
    24781 cgcaacgcgg ggcgtttgag attttattat gctaatcaaa ttgttaacta aagttttcgg
    24841 tagtcgtaac gatcgcaccc tgcgccggat gcgcaaagtg gtcaacatca tcaatgccat
    24901 ggaaccggag atggaaaaac tctccgacga agaactgaaa gggaaaaccg cagagtttcg
    24961 tgcacgtctg gaaaaaggcg aagtgctgga aaatctgatc ccggaagctt tcgccgtggt
    25021 acgtgaggca agtaagcgcg tctttggtat gcgtcacttc gacgttcagt tactcggcgg
    25081 tatggttctt aacgaacgct gcatcgccga aatgcgtacc ggtgaaggaa aaaccctgac
    25141 cgcaacgctg cctgcttacc tgaacgcact aaccggtaaa ggcgtgcacg tagttaccgt
    25201 caacgactac ctggcgcaac gtgacgccga aaacaaccgt ccgctgtttg aattccttgg
    25261 cctgactgtc ggtatcaacc tgccgggcat gccagcaccg gcaaagcgcg aagcttacgc
    25321 agctgacatc acttacggta cgaacaacga atacggcttt gactacctgc gcgacaacat
    25381 ggcgttcagc cctgaagaac gtgtacagcg taaactgcac tatgcgctgg tggacgaagt
    25441 ggactccatc ctgatcgatg aagcgcgtac accgctgatc atttccggcc cggcagaaga
    25501 cagctcggaa atgtataaac gcgtgaataa aattattccg cacctgatcc gtcaggaaaa
    25561 agaagactcc gaaaccttcc agggcgaagg ccacttctcg gtggacgaaa aatctcgcca
    25621 ggtgaacctg accgaacgtg gtctggtgct gattgaagaa ctgctggtga aagagggcat
    25681 catggatgaa ggggagtctc tgtactctcc ggccaacatc atgctgatgc accacgtaac
    25741 ggcggcgctg cgcgctcatg cgctgtttac ccgtgacgtc gactacatcg ttaaagatgg
    25801 tgaagttatc atcgttgacg aacacaccgg tcgtaccatg cagggccgtc gctggtccga
    25861 tggtctgcac caggctgtgg aagcgaaaga aggtgtgcag atccagaacg aaaaccaaac
    25921 gctggcttcg atcaccttcc agaactactt ccgtctgtat gaaaaactgg cggggatgac
    25981 cggtactgct gataccgaag ctttcgaatt tagctcaatc tacaagctgg ataccgtcgt
    26041 tgttccgacc aaccgtccaa tgattcgtaa agatctgccg gacctggtct acatgactga
    26101 agcggaaaaa attcaggcga tcattgaaga tatcaaagaa cgtactgcga aaggccagcc
    26161 ggtgctggtg ggtactatct ccatcgaaaa atcggagctg gtgtcaaacg aactgaccaa
    26221 agccggtatt aagcacaacg tcctgaacgc caaattccac gccaacgaag cggcgattgt
    26281 tgctcaggca ggttatccgg ctgcggtgac tatcgcgacc aatatggcgg gtcgtggtac
    26341 agatattgtg ctcggtggta gctggcaggc agaagttgcc gcgctggaaa atccgaccgc
    26401 agagcaaatt gaaaaaatta aagccgactg gcaggtacgt cacgatgcgg tactggaagc
    26461 aggtggcctg catatcatcg gtaccgagcg tcacgaatcc cgtcgtatcg ataaccagtt
    26521 gcgcggtcgt tctggtcgtc agggggatgc tggttcttcc cgtttctacc tgtcgatgga
    26581 agatgcgctg atgcgtattt ttgcttccga ccgagtatcc ggcatgatgc gtaaactggg
    26641 tatgaagcca ggcgaagcca ttgaacaccc gtgggtgact aaagcgattg ccaacgccca
    26701 gcgtaaagtt gaaagccgta acttcgacat tcgtaagcaa ctgctggaat atgatgacgt
    26761 ggctaacgat cagcgtcgcg ccatttactc ccagcgtaac gaactgttgg atgtcagcga
    26821 tgtgagcgaa accattaaca gcattcgtga agatgtgttc aaagcgacca ttgatgccta
    26881 cattccacca cagtcgctgg aagaaatgtg ggatattccg gggctgcagg aacgtctgaa
    26941 gaacgatttc gacctcgatt tgccaattgc cgagtggctg gataaagaac cagaactgca
    27001 tgaagagacg ctgcgtgacg gcattctggc gcagtccatc gaagtgtatc agcgtaaaga
    27061 agaagtggtt ggtgctgaga tgatgcgtca cttcgagaaa ggcgtcatgc tgcaaacgct
    27121 tgactccctg tggaaagagc acctggcagc gatggactat ctgcgtcagg gtatccacct
    27181 gcgtggctac gcacagaaag atccgaagca ggaatacaaa cgtgaatcgt tctccatgtt
    27241 tgcagcgatg ctggagtcgt tgaaatatga agttatcagt acgctgagca aagttcaggt
    27301 acgtatgcct gaagaggttg aggagctgga acaacagcgt cgtatggaag ccgagcgttt
    27361 agcgcaaatg cagcagctta gccatcagga tgacgactct gcagccgcag ctgcactggc
    27421 ggcgcaaacc ggagagcgca aagtaggacg taacgatcct tgcccgtgcg gttctggtaa
    27481 aaaatacaag cagtgccatg gccgcctgca ataaaagcta actgttgaag taaaaggcgc
    27541 aggattctgc gcctttttta taggtttaag acaatgaaaa agctgcaaat tgcggtaggt
    27601 attattcgca acgagaacaa tgaaatcttt ataacgcgtc gcgcagcaga tgcgcacatg
    27661 gcgaataaac tggagtttcc cggcggtaaa attgaaatgg gtgaaacgcc ggaacaggcg
    27721 gtggtgcgtg aacttcagga agaagtcggg attacccccc aacatttttc gctatttgaa
    27781 aaactggaat atgaattccc ggacaggcat ataacactgt ggttttggct ggtcgaacgc
    27841 tgggaagggg agccgtgggg taaagaaggg caacccggtg agtggatgtc gctggtcggt
    27901 cttaatgccg atgattttcc gccagccaat gaaccggtaa ttgcgaagct taaacgtctg
    27961 taggtcagat aaggcgtttt cgccgcatcc gacattcgca cacgatgcct gatgcgacgc
    28021 tggcgcgtct tatcaggcct aaagggattt ctaactcatt gataaatttg tttttgtagg
    28081 tcggataagg cgttcacgcc gcatccgaca tttgcacaag atgcctgatg cgacgctgtc
    28141 cgcgtcttat caggcctacg tgcggcatca gacaaatgtc actgctttgg ttcttcgctc
    28201 cagtcatcgc tttcggaaag atcgccactg ctggggattc gtttttcttc agcagcccat
    28261 tctccgaggt cgatcag
//