From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: N.sylvestris mRNA for 3-hydroxy-3-methylglutaryl-coenzyme A
Date: 29 Dec 92 01:02:50 GMT
Distribution: bionet
Checksum: 35770 6

LOCUS       TOBHMGRNA    2355 bp ss-RNA             PLN       04-DEC-1992
DEFINITION  N.sylvestris mRNA for 3-hydroxy-3-methylglutaryl-coenzyme A
            reductase
ACCESSION   X63649
KEYWORDS    HMGR gene; hydroxymethylglutaryl-CoA reductase; mevalonate.
SOURCE      Nicotiana sylvestris RNA.
  ORGANISM  Nicotiana sylvestris
            Eukaryota; Plantae; Embryobionta; Magnoliophyta; Magnoliopsida;
            Asteridae; Solanales; Solanaceae.
REFERENCE   1  (bases 1 to 2355)
  AUTHORS   Genschik,P., Criqui,M.C., Parmentier,Y., Marbach,J., Durr,A.,
            Fleck,J. and Jamet,E.
  TITLE     Isolation and characterization of a cDNA encoding a 3-hydroxy-3
            methylglutaryl coenzyme A reductase from Nicotiana sylvestris
  JOURNAL   Plant Mol. Biol. 20, 337-341 (1992)
  STANDARD  full automatic
COMMENT     *source: stage=protoplasts; *source: clone_library=lambda gt10 cDNA
            library; *source: clone=6P2;
               From EMBL    entry NSHMGRNA;  dated 04-AUG-1992.
FEATURES             Location/Qualifiers
     polyA_signal    1999..2005
     polyA_signal    2032..2037
     CDS             87..1901
                     /function="catalyses synthesis ofmevalonate"
                     /gene="HMGR"
                     /note="endoplasmic reticulum location"
                     /product="3-hydroxy-3-methylglutaryl-coenzyme Areductase"
                     /codon_start=1
                     /translation="MDVRRRSEKPAYPTKEFAAGEKPLKPHKQQQEQDNSLLIASDAL
                     PLPLYLTNGLFFTMFFSVMYYLLSRWREKIRNSTPLHVVTFSELVAIASLIASVIYLL
                     GFFGIGFVQSFVSRDNNDECWDEEDENDEQFLLEEDSRRGPATTLGCTAVPPPPALQI
                     VPMVPPQPSKVAAMSEKPAPLVTPAASEEDEEIIKSVVQGKMPSYSLESKLGDCKRAA
                     SIRKEALQRITGKSLEGLPLEGFDYESILGQCCEMPIGYVQIPVGIAGPLLLDGREYS
                     VPMATTEGCLVASTNRGCKAIYASGGATSVLLRDGMTRAPCVRFGTAKRAAELKFFVE
                     DPVKFETLAAVFNQSSRFARLQRIQCAIAGKNLYMRFVCSTGDAMGMNMVSKGVQNVL
                     DYLQNEYPDMDVIGISGNFCSDKKPAAVNWIEGRGKSVVCEAIITEEVVKKVLKTEVA
                     ALVELNMLKNLTGSAMAGALGGFNAHASNIVSAVYIATGQDPAQNIESSHCITMMEAV
                     NDGKDLHVSVTMPSIEVGTVGGGTQLASQSACLNLLGVKGANREVPGSNARLLATIVA
                     GSVLAGELSLMSAISAGQLVKSHMKYNRSTKDVTKASS"
BASE COUNT      602 a    510 c    581 g    662 t
ORIGIN      
        1 caaatcacac acaacaatcc cacagttccc ctcttcctct tttctctccc ttcacttccc
       61 ggccggcggc aatcttaccg gtgaaaatgg acgttcgccg gagatctgag aagcctgcat
      121 atccaaccaa agaatttgcc gccggcgaaa aacctctcaa accccacaaa caacaacaag
      181 aacaggacaa ctcccttctc attgcctccg atgccctccc actccctttg tacctcacaa
      241 atgggttgtt tttcaccatg tttttctccg ttatgtatta tcttctcagc aggtggcgtg
      301 agaaaatcag gaactccact cctctccacg tggttacttt ttctgaatta gttgccattg
      361 cttcattgat tgcttccgtg atttatcttc tggggttctt cgggatcggg tttgttcagt
      421 cgttcgtttc cagggataat aatgatgaat gttgggatga agaggatgaa aacgatgagc
      481 aatttctctt ggaagaagat agtcgtcgtg gacctgcaac tactcttggc tgcactgctg
      541 ttccaccacc acctgctcta caaattgtcc caatggtacc accgcaacct tccaaggtcg
      601 cagctatgtc cgaaaaacct gcgcccttgg ttacaccagc agcgtctgag gaagacgagg
      661 agatcattaa atccgtggtg caggggaaaa tgccgtcgta ctctttggaa tcgaaactcg
      721 gtgattgtaa gagagctgct tccattcgta aagaggcgtt gcagaggatt acggggaagt
      781 ctctagaagg gctccctctg gagggatttg attatgaatc cattcttggg cagtgctgtg
      841 agatgccaat cggttacgtg cagatacccg ttggaatagc cgggccgttg ttgctcgacg
      901 ggagagagta ttcggtacca atggcaacca ctgaaggatg tttagtggct agcaccaaca
      961 ggggttgcaa ggctatctat gcttctggcg gtgccactag cgtgttgctc cgcgatggga
     1021 tgaccagagc accttgtgtc aggtttggca ctgccaaaag ggcggcggag ttgaagttct
     1081 ttgttgaaga tcctgtcaaa tttgagacac ttgctgctgt tttcaaccag tcaagcagat
     1141 ttgccagatt acaaaggatt caatgcgcaa ttgcgggaaa gaatctatac atgagatttg
     1201 tgtgtagcac tggtgatgca atgggaatga acatggtgtc aaaaggtgta caaaatgttc
     1261 ttgattacct ccagaatgaa tatcccgaca tggatgtcat cggcatatct gggaactttt
     1321 gctcggacaa gaagccagca gcagttaact ggattgaggg gagaggaaag tctgtagttt
     1381 gtgaggcaat tatcacggaa gaggtggtga agaaagttct taaaactgag gttgctgctc
     1441 ttgtggagct gaacatgctt aaaaatctta ctggctctgc gatggctggt gcacttggtg
     1501 gtttcaatgc ccacgccagc aatatcgtct cagctgtgta tatagcaact ggtcaggacc
     1561 cagctcagaa catagagagc tctcattgta tcactatgat ggaggctgta aatgatggca
     1621 aggacctcca tgtttctgtt acaatgcctt ccattgaggt tggtactgtt ggaggtggaa
     1681 ctcagcttgc ttctcagtca gcttgcttga acttattggg agtgaaaggt gccaacagag
     1741 aggtaccagg gtcaaatgca agactcttgg ccacaatagt tgctggttct gttcttgctg
     1801 gggagttatc cctcatgtca gctatctcag ctgggcagct ggttaagagc cacatgaaat
     1861 acaacagatc taccaaagat gtcacaaagg catcctccta atgaggggaa gacaatccaa
     1921 ttccccacct tgtacttgaa gctgtacaca ggtgctccaa caagtagctt ccacttaaga
     1981 atgatcaaga gtagtaataa ataaaattta caaagcaaaa cctataatag aagtcagaag
     2041 acaaataacg ccaacttctt atttttggtg aagtcatgtg aaggtggtag ggtctctttt
     2101 cctcatagtt agggtaaaga aacctgaaaa tgtattgtga agatccgctg ggggaagtta
     2161 gctgggctgt ctgttgtctc agtgattctt gatgccattt gttagctgcc aacacttggt
     2221 atctttcaat gtgccatagg gggaatatcc ttttcatttc tgttgtattg ttctttcgtt
     2281 tgtcaatttg tcatttgttt gtaatttctg ctggggttgt ctgggtctga tcagcaagtg
     2341 tgatcttgta tatga
//