From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Butyrivibrio fibrisolvens glutamine synthetase (glnA) gene,
Date: 3 Jan 93 22:25:49 GMT
Distribution: bionet
Checksum: 10826 6

LOCUS       BUTGLNA      2423 bp ds-DNA             BCT       24-DEC-1992
DEFINITION  Butyrivibrio fibrisolvens glutamine synthetase (glnA) gene,
            complete cds.
ACCESSION   L08256
KEYWORDS    ammonia assimilation; glnA gene; glutamine synthetase.
SOURCE      Butyrivibrio fibrisolvens (strain H17c) DNA.
  ORGANISM  Butyrivibrio fibrisolvens
            Prokaryotae; Gracilicutes; Scotobacteria; Anaerobic gram-negative
            straight, curved and helical rods; Bacteroidaceae.
REFERENCE   1  (bases 1 to 2423)
  AUTHORS   Goodman,H.J. and Woods,D.R.
  TITLE     Cloning and nucleotide sequence of the Butyrivibrio fibrisolvens
            gene encoding a type III glutamine synthetase
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     RBS             187..195
                     /gene="glnA"
     CDS             201..2303
                     /EC_number="6.3.1.2"
                     /gene="glnA"
                     /product="glutamine synthetase"
                     /codon_start=1
                     /translation="MIEASKLTTEFGSLVFNDKIMKERLPKDIYKAVHKTIEKEPHLE
                     PGCSYSCSSNHEGVGNREQCYHFTPPGSSPMTGLTAEKHDSFISPTEDGRSSWSSQEK
                     SWLRANLMHQASQWWSSCHNSSMRGYQHGILHHHAFIKDGSLLLPTAFCSYGGEALDR
                     DSLLRSMEALSNEAVKMMRLLGYEDVNRVNTTIGSEQEYFLIDKDFYKKRKDLLLTGR
                     TLIGAPASKGQEMEDHYFGVIKPKVSAYMHDLDEELWKLGIPAKTKHNEVAPSQHELA
                     PVFETANIAVDHNQLTMEVMKKVADKHNYACLLHEKPFEGVNGSGKHNNWSICTDTGI
                     NLLDPGKNPGENIPFLVFLMSVIAAVDEYAPILRLSVASAGNDHRLGGNEAPPAIISI
                     FVGDELAEVLKAVEAGEAYKAAGKSQMTWEQQYFTFTKDNTDRNRTSPFAFTGNKFES
                     DGGHSSVANGKYGPQHMQLQKEVATLNAKLSAYSGDELKEKVKEVLKETLLAHKRVLF
                     NGNGYTDEWVEEAAKRGLPNLKALPDCMPYWISDESIDLFTRHGIFTKEEIYSRYEIL
                     LENYSKSIHIESLTMQEMIRKDLTEGLVAYEKDLSKEIVQKKSLLDGDCCALELGVLK
                     SLDKSSAEMGKALSKLFEDTKKAEGMTEALETASYYESTVLADMDELRKYADEAEALI
                     PEKYLSYPTYGEMLFSLR"
BASE COUNT      763 a    503 c    566 g    591 t
ORIGIN      
        1 tactaaggag tcactgttat acagagaaat atttgaaaaa tactatccgg gccagtcaga
       61 tatggttatt gatttctgga tgcctaacaa gagctggaag ggctgcaatg taacagatcc
      121 ttcagcaaga gttttatcga attatggagc cagcggtcag taaaggctca taaataaaag
      181 aaaaacggag gagagtggca atgatcgaag cgagcaaatt aacaacagaa tttggaagtc
      241 tagtatttaa cgacaagatt atgaaggaac gccttcccaa ggatatctac aaggcagttc
      301 ataagacaat tgaaaaggaa ccacatcttg agcctggatg tagctactcg tgtagcagca
      361 accatgaagg agtgggcaat cgagaacagt gctaccactt caccccacct ggttccagcc
      421 caatgacagg tcttacagct gaaaagcacg acagtttcat ttctccaaca gaagatggca
      481 gatcatcatg gagttctcag gaaaagagct ggttaagggc gaacctgatg catcaagctt
      541 cccagtggtg gtcttcgtgt cacaattcga gcatgcgagg ttatcagcat gggatccttc
      601 atcaccatgc attcatcaag gatggttccc ttttacttcc aacagctttc tgctcatacg
      661 gcggagaagc tcttgataga gactcacttc ttcgttcaat ggaggctctt tcaaatgaag
      721 cggttaagat gatgcgtctt cttggatatg aggatgttaa ccgcgtaaat actacaatcg
      781 gttcagaaca ggaatatttc cttatcgaca aggatttcta taagaagagg aaagatttgc
      841 ttcttacagg cagaacactt atcggtgctc cagccagcaa gggtcaggaa atggaagatc
      901 actacttcgg agttattaag cctaaggtat ctgcatacat gcatgacctt gatgaagagc
      961 tctggaagct tggaatccct gctaagacca agcacaacga ggttgcacct tctcagcacg
     1021 agctggctcc tgtatttgag acagcaaaca tcgctgtaga tcacaaccag ctcacaatgg
     1081 aagttatgaa gaaggttgct gacaagcaca actatgcatg tcttcttcac gagaagccat
     1141 ttgagggagt taacggatct ggtaagcaca ataactggtc aatctgcaca gatacaggaa
     1201 tcaaccttct tgatccaggt aagaacccag gcgagaacat tccttttctt gtctttctta
     1261 tgtctgttat cgcagcagtt gatgagtatg caccaatcct cagactttca gtagcatctg
     1321 ccggaaacga ccacagactt ggaggaaacg aggcacctcc agcaatcatc tctatcttcg
     1381 ttggtgatga gcttgcagaa gtactcaagg ctgttgaagc cggagaagct tacaaggcag
     1441 caggcaagtc tcagatgaca tgggagcaac agtacttcac cttcaccaag gacaacacag
     1501 acagaaacag aacatcacct tttgctttca ctggtaacaa gttcgagtca gatggtggtc
     1561 acagttctgt tgctaacggt aaatatggtc ctcaacacat gcagttgcag aaggaagtag
     1621 caacgcttaa tgcaaagctt tcagcataca gcggagatga gcttaaagag aaagttaagg
     1681 aagttcttaa agagacactt cttgcacata agagagttct tttcaacgga aacggatata
     1741 cagacgagtg ggttgaggaa gctgctaaga gaggccttcc aaaccttaag gctcttccag
     1801 attgcatgcc atactggatc agcgatgagt caatagatct tttcacaaga cacggtatct
     1861 tcaccaagga agagatttac tcaagatatg agatcctcct tgagaactat tctaagtcaa
     1921 ttcatattga gtctctcaca atgcaggaga tgatcagaaa agacttaaca gaaggtcttg
     1981 tagcatatga aaaagatctg tcaaaagaga ttgttcagaa aaagagcctt ctcgatggag
     2041 actgctgtgc acttgagctg ggagttctta agtctcttga taagtccagc gcagagatgg
     2101 gcaaggcact ttcaaagctc ttcgaggata ccaagaaggc agagggcatg acagaagctc
     2161 ttgagacagc aagttactat gagagcacag tccttgcaga tatggatgag cttagaaagt
     2221 acgctgatga ggcagaggca cttattccag agaagtacct tagctatcca acatacggag
     2281 agatgctgtt ctcacttaga taatctgagt gccgcgtaaa tatcataaat gattaggggc
     2341 gattattgcg gatccaaata caattatcgc cccataattg catatttagt tacaagggaa
     2401 ggagagatac atccacatgg aac
//