From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Escherichia coli restriction-modification enzyme type I M subunit
Date: 31 Dec 92 01:44:26 GMT
Distribution: bionet
Checksum: 35828 4

LOCUS       ECOHSDMB     1590 bp ds-DNA             BCT       18-DEC-1992
DEFINITION  Escherichia coli restriction-modification enzyme type I M subunit
            (hsdM) gene, complete cds.
ACCESSION   L02508
KEYWORDS    hsdM gene; restriction-modification enzyme type I M subunit;
            restriction-modification system type I M subunit.
SOURCE      Escherichia coli (strain B) DNA.
  ORGANISM  Escherichia coli
            Prokaryotae; Gram-negative facultatively anaerobic rods;
            Enterobacteriaceae.
REFERENCE   1  (bases 1 to 1590)
  AUTHORS   Sharp,P.M., Kelleher,J.E., Daniel,A.S., Cowan,G.M. and Murray,N.E.
  TITLE     Roles of selection and recombination in the evolution of type I
            restriction-modification systems in enterobacteria
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 89, 9836-9840 (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     CDS             1..1590
                     /gene="hsdM"
                     /citation=[1]
                     /note="homologue to X06545, and to X13145; putative"
                     /product="restriction-modification enzyme type I M
                     subunit"
                     /codon_start=1
                     /translation="MNNNDLVAKLWKLCDNLRDGGVSYQNYVNELASLLFLKMCKETG
                     QEAEYLPEGYRWDDLKSRIGQEQLQFYRNLLVHLGADNQKLVQAVFQNVNTTITQPKQ
                     LTELVSNMDSLDWYNGAHGKSRDDFGDMYEGLLQKNANETKSGAGQYFTPRPLIKTII
                     HLLKPQPREVVQDPAAGTAGFLIEADRYVKSQTNDLDDLDGDTQDFQIHRAFIGLELV
                     PGTRRLALMNCLLHDIEGNLDHGGAIRLGNTLGSDGENLPKAHIVATNPPFGSAAGTN
                     ITRTFVHPTSNKQLCFMQHIIETLHPGGRAAVVVPDNVLFEGGKGTDIRRDLMDKCHL
                     HTILRLPTGIFYAQGVKTNVLFFTKGTVANPHQDKNCTDDVWVYDLRTNMPSFGKRTP
                     FTDEHLQPFERVYGEDPHGLSPRSEGEWSFNAEETEVADSEENKNTDQHLATSRWRKF
                     TREWIRTTKSDSLDISWLKDKDSIDADNLPEPDVLAAEAMGELVQALGELDALIRELG
                     ASDEADAQRQLLEEAFGGVKE"
BASE COUNT      395 a    423 c    463 g    309 t
ORIGIN      
        1 atgaacaata acgatctggt cgcgaagctg tggaaactgt gcgacaacct gcgcgatggc
       61 ggcgtttcct atcaaaacta cgtcaatgaa ctcgcctcgc tgctgttttt gaaaatgtgt
      121 aaagaaaccg gacaggaagc ggaatacctg ccggaaggct accgctggga tgacctgaaa
      181 tcccgcatcg gccaggagca gttgcagttc taccgtaacc tgctggtgca tctgggcgcc
      241 gacaatcaaa agctggtgca ggcggtgttc cagaacgtca acaccaccat tacccagccg
      301 aaacagttga ccgaactggt cagcaatatg gattcactgg actggtacaa cggcgcgcac
      361 ggtaagtcac gcgatgactt cggcgatatg tacgaagggc tgttgcagaa aaacgccaac
      421 gaaaccaagt ctggcgcggg ccagtacttc accccacgtc cgctgattaa aaccattatt
      481 catctgctga aaccgcagcc gcgtgaagtg gtgcaggacc cggcagcagg tacagcgggc
      541 tttttgattg aagctgaccg ctacgttaag tcgcagacta acgatctgga cgaccttgat
      601 ggcgacacgc aggatttcca gatccaccgc gcgtttatcg gcctcgaact ggtacccggc
      661 acccgtcgtc tggcgctaat gaactgcctg ctgcacgata tcgaaggcaa cctcgaccac
      721 ggtggcgcaa tccgtctggg caacaccctg ggtagcgacg gtgaaaacct gccgaaggcg
      781 catattgtcg ccactaaccc gccgtttggt agcgccgcag gcaccaacat tacccgtacc
      841 tttgttcacc cgaccagcaa caaacaattg tgctttatgc agcatattat cgaaacactg
      901 caccccggcg gtcgtgcagc ggtggtggtg ccggataacg tgctgtttga aggcggcaaa
      961 ggcacggaca tccgtcgtga cctgatggat aagtgtcatc tgcacactat tctgcgtctg
     1021 ccgaccggta ttttttacgc gcagggcgtg aagacgaacg tgctgttctt taccaaaggg
     1081 acggtggcga acccgcatca ggataagaac tgtaccgatg atgtgtgggt gtacgacctg
     1141 cgtaccaata tgccgagctt cggcaaacgc acgccgttta ccgacgagca tctgcagccg
     1201 tttgagcgcg tgtatggcga agatccgcac ggtttaagcc cgcgtagcga aggggaatgg
     1261 agttttaacg ccgaagagac ggaagttgcc gacagcgaag agaacaaaaa caccgaccag
     1321 cacctggcta ccagccgctg gcgtaagttc acccgcgagt ggatccgcac cacgaaatcc
     1381 gattcgctgg atatctcctg gctgaaagat aaagatagca ttgatgccga caacctgccg
     1441 gagccggatg tattagcggc agaagcgatg ggcgagctgg tacaggcgct gggcgaactg
     1501 gatgcgctga tacgtgaact gggagcgagc gatgaggcgg atgcacagcg tcagttgctg
     1561 gaagaagcgt ttggtggggt gaaggaatga
//