From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Toxoplasma gondii antigen p28 gene, complete cds.
Date: 21 Aug 92 10:13:03 GMT
Distribution: bionet
Checksum: 40059     5

LOCUS       TOXP28ANT    1936 bp ds-DNA             INV       21-AUG-1992
DEFINITION  Toxoplasma gondii antigen p28 gene, complete cds.
ACCESSION   M99392
KEYWORDS    antigen p28.
SOURCE      Toxoplasma gondii (strain RH) (library: C2XB library of J.L. Burg)
            tachyzoite DNA.
  ORGANISM  Toxoplasma gondii
            Eukaryota; Animalia; Protozoa; Apicomplexa; Perkinsea; Coccidia;
            Eucoccidiidae; Eimeriina; Sarcocystidae.
REFERENCE   1  (sites)
  AUTHORS   Prince,J.B., Araujo,F.G., Remington,J.S., Burg,J.L., Boothroyd,J.C.
            and Sharma,S.D.
  TITLE     Cloning of cDNAs encoding a 28 kilodalton antigen of Toxoplasma
            gondii
  JOURNAL   Mol. Biochem. Parasitol. 34, 3-13 (1989)
  STANDARD  full staff_review
REFERENCE   2  (bases 1 to 1936)
  AUTHORS   Parmley,S.F., Sgarlato,G.D. and Remington,J.S.
  TITLE     Genomic and corrected cDNA sequence of the P28 gene from Toxoplasma
            gondii
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     CDS             join(733..881,1127..1535)
                     /product="antigen p28"
                     /codon_start=1
                     /translation="MFAVKHCLLVVAVGALVNVSVRAAEFSGVVNQGPVDVPFSGKPL
                     DERAVGGKGEHTPPLPDERQQEPEEPVSQRASRVAEQLFRKFLKFAENVGHHSEKAFK
                     KAKVVAEKGFTAAKTHTVRGFKVAKEAAGRGMVTVGKKLANVESDRSTTTTQAPDSPN
                     GLAETEVPVEPQQRAAHVPVPDFSQ"
     mRNA            join(627..881,1127..1921)
     old_sequence    replace(1..279,"t")
                     /note="the new gene sequence contains 732-bp of 5'
                     untranslated sequences including the addition of sequences
                     1..639'"
                     /citation=[2]
                     /citation=[1]
     exon            627..881
                     /number=1
     intron          882..1126
     exon            1127..1921
                     /number=2
     polyA_site      1921
BASE COUNT      491 a    467 c    524 g    454 t
ORIGIN
        1 tgctgcaggc agccaggtag gtggaacaga tctccagcga gtacgaccac tgtgcgtgta
       61 cttcgccaaa aggaaaatac acctgcaccg ctattaagcg cagtcgtcta cctgaatcgt
      121 ctccccggcc tcatcatttt gttcgacaca agtttccatt aggactttgt gacagtcgtc
      181 tgctctcgac ctttccaacc gtctcgtagg cgaaaaatcc tcgggtgact tgccgtggac
      241 gaacgcctcc cgtttgcttc atcaagtagc tatgcgatag gttccgcagt gcagccaggc
      301 ttgcgaaaaa caagttcgtc gcaaaaggtt aattacctac gcaccacgaa ggaaaacgcg
      361 tatcacgtca gtccttacgg tcaatataca aatacttggg ccgtccagtg gaggcaacgt
      421 gcccgtcgca cggtgatact gactggtgac ttgcacgtac gtctctcgcc ggcgtccaaa
      481 ccaaattgac ccggggcagc ctactccctg tcgtccctta ggctaagtgc gagcaacatc
      541 tctacacaga gacgacgcca gagacgcaaa atgaacagcg gaacctgcgt cgctgtctgt
      601 cctgcgaact gatgacagaa agggtcatta aacgatttct tttgcaattc gcgtcgttat
      661 cgcacgttgt ttctcttccc acgaatagtt gttttgatta gatattgctt cttctccaca
      721 tatcgcctca caatgttcgc cgtaaaacat tgtttgctgg ttgttgccgt tggcgccctg
      781 gtcaacgtct cggtgagggc tgccgagttt tccggagttg ttaaccaggg accagtcgac
      841 gtgcctttca gcggtaaacc tcttgatgag agagcagttg ggtaagttgg caaaagtaat
      901 gatagaggca ggggttgaac gataggcggc tgcagatttg tataacacaa catgatgtag
      961 ctgccacggt tttttttcgg agagtgatgc cgtctgactg ttcatcgcac ccatgggagc
     1021 tagggaggtg cgctttctgt gtgatatgta ttgtcctagt ccaatttccc acgcactgta
     1081 gtgtcttgag actcggtgcc atgtagaatt ttgtgtctgc ctgcagagga aaaggtgaac
     1141 atacaccacc actcccagac gagaggcaac aagagccaga agaaccggtt tcccaacgtg
     1201 catccagagt ggcagaacaa ctgtttcgca agttcttgaa gttcgctgaa aacgtcggac
     1261 atcacagtga gaaggccttc aaaaaagcaa aggtggtggc agaaaaaggc ttcaccgcgg
     1321 caaaaacgca cacggttagg ggtttcaagg tggccaaaga agcagctgga aggggcatgg
     1381 tgaccgttgg caagaaactc gcgaatgtgg agagtgacag aagcactacg acaacgcagg
     1441 cccccgacag ccctaatggc ctggcagaaa ccgaggttcc agtggagccc caacagcggg
     1501 ccgcacacgt gcccgtccca gacttttcgc agtaatgttg actacgacga aagtgatgcg
     1561 caggctggaa agccgctgaa ggtctacaaa gccgatcagt gaaaaatgtg tggggaggtg
     1621 gtcttgttgc aggaatgcaa tggtgttaag catcgtgttc gaatgcagtg cgtgtatcag
     1681 ttgtgcgcgg aaggacactg cttcaatgtt aagaacctgt tttctccgta gagaggacca
     1741 aaagacgatt gcaaaactgg tatgtacgca atagcccaat gccggacgtc agttggttgt
     1801 atgtgacgct cccagatgtc atatgccttg tgagtgtgtc tgggatgcaa gtttttggtg
     1861 tgcgttgatt tcgccagctt atgacagtgg cagacgaatt attgacatga tacaaggacg
     1921 cagaaaggaa caaaca
//