From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Trypanosoma cruzi DNA topoisomerase II (TOP2) gene, complete cds.
Date: 22 Dec 92 22:57:27 GMT
Distribution: bionet
Checksum: 01714 8

LOCUS       TRBTOPOII    3977 bp ds-DNA             INV       30-NOV-1992
DEFINITION  Trypanosoma cruzi DNA topoisomerase II (TOP2) gene, complete cds.
ACCESSION   M91165
KEYWORDS    DNA topoisomerase II.
SOURCE      Trypanosoma cruzi (strain Dm28) epimastigote DNA.
  ORGANISM  Trypanosoma cruzi
            Eukaryota; Animalia; Protozoa; Sarcomastigophora; Mastigophora;
            Kinetoplastida; Trypanosomatina; Trypanosomatidae.
REFERENCE   1  (bases 1 to 3977)
  AUTHORS   Fragoso,S.P. and Goldenberg,S.
  TITLE     Cloning and characterization of the gene encoding Trypanosoma cruzi
            DNA topisomerase II
  JOURNAL   Mol. Biochem. Parasitol. 55, 127-134 (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     CDS             66..3764
                     /function="relaxation of supercoiled DNA"
                     /gene="TOP2"
                     /note="putative"
                     /product="DNA topoisomerase II"
                     /codon_start=1
                     /translation="MAEASKYKKLTPIDHVLIRPEMYVGSVDTSSSSMFVFDHEKGRM
                     VWESLKVNHGLLKIVDEILLNASDNIANKGGRMTYIRVHITEAGEITIENDGAGIPIV
                     RSKEHKLYIPEMVFGHLLTSSNYDDTSQNAVAGRHGYGAKLTNILSHRFSVCCRTKGK
                     EFHMSWHDHMRRATAPRVSNVDPKEKNLTRVKFLPDYERFGLDANKISHDMKRVLHKR
                     IMDLAAMFPSIEISLNGVPFAFKSFADYAMLYSSPSSSGEMPPAPFVYESRNGAIAFI
                     PSLTAGTRRIFGVVNGVVTHNGGTHCNAAQEVLQSSLESVEKALKKDNKVIDTNRVLR
                     HFMILVFLVQVQPKFDSQNKARLVSVPTMPRVPRQELMDFLLRMPFLEAHVNTVTGQL
                     ADELNKEMGAGRRMSSKSLISSITKLVDATTTRRDPRFVRTLIVTEGDSAKALAQNSL
                     SSDQKRYTGVFPLRGKLLNVRNKNLKRLKNCKELQELFCALGLELGKIYKDAEELRYQ
                     RLLVMTDQDADGSHIKGLVINAFEALWPSLLNRNPGFISIFSTPIVKVRLRDKSTHSF
                     FSLKEFHKWQKTHGNVSYTAKYYKALGTSTTAEGKEYFKDMDKHTMRLVVERNDHKLL
                     DSVFDSQEVEWRKDWMTKANAYTGEVDIDRSKKTLTVPDFVHKEMVHFALAGNARALA
                     HAVDGLKPSQRKILWAIMRRSGNESAKVAQLSGYISEVSAFHHGEMSLQETIIKMAQN
                     FTGGNNINLLIPEGQFGSRQQLGNDHAAARYIFTKLSSLARILFPSEDEPLLDYVTEE
                     GQQVEPNHYVPILPLLLCNGSVGIGFGFASNIPPFHPLDVSAAVRSMINGEAAKVVVR
                     RLVPWAVGYQGEVRRGPEGEFIAAGSYQYYVDGRVHVTEIPWTLSIEAFRDHISVLAS
                     KDVVQRIADYSGANHVDIDLELTNGAMTTYAECESELSLTQRIYINGTVFSPTGVLTP
                     LEGDLAPVLQWHYDRRLDLYKKRRQRNLGLLEAELAREKSTLKFVTHFREGKIDIVNA
                     TDDSLAKTCSKLGMVRVDDSYDYVLRKPITFYTKTSLENLNRKISETEKRIDKLKKTA
                     PVQMWLDELDRFDRAFEEHENTAVATILKERRVNPPTGDVSRNLQQPRLELEEVKVSS
                     SGGKSVPMRVRVRKYVPPPPSKRPHVGQSVGGGGGGGSVRSSAAAVVAHVKAEKKAAR
                     ARSMQKMLLDVVARQVARVLPRLPWFLF"
BASE COUNT     1052 a    948 c   1063 g    914 t
ORIGIN      
        1 aaaaaatata tatttgacgt gtttgtgttg gggaaaaaca gagaagaaga ggggaggggg
       61 agcgaatggc ggaggcatcg aagtacaaga aactcacccc catcgaccat gtcctcatac
      121 ggccggagat gtacgttggc agcgtggata catcctccag ttccatgttt gtctttgatc
      181 atgagaaggg ccgcatggtg tgggagtcac tgaaggtcaa ccacggcctc ctgaagatcg
      241 tggatgaaat tctcctcaac gcctccgaca acatcgcaaa caaaggcggg cgcatgacgt
      301 acatccgcgt tcacatcacg gaggctgggg aaatcacaat agagaatgac ggtgccggta
      361 tccccattgt acgcagcaaa gaacataaac tttacatccc agagatggtt tttggccacc
      421 ttctcaccag ctccaactac gacgacacga gtcagaatgc cgtcgcagga cgtcacgggt
      481 acggtgcaaa actaacgaat attttgtctc accgcttttc cgtctgctgc cgcacaaaag
      541 ggaaagagtt tcatatgagt tggcacgatc atatgcgcag ggcgacagcc ccacgtgtgt
      601 cgaacgtcga cccaaaagag aagaatctca cacgggtgaa gtttcttcca gactacgaac
      661 gctttggatt ggacgcgaat aaaatctcac acgacatgaa gcgtgtgctg cacaagcgca
      721 tcatggatct tgccgcgatg tttccaagca ttgaaatatc actgaacggc gtcccgtttg
      781 cctttaaatc ctttgctgac tacgccatgt tgtactcttc ccccagttcc agtggagaaa
      841 tgccaccagc accgtttgtg tacgagagcc gcaatggtgc cattgcgttt attccctccc
      901 ttaccgccgg cacacgtcgt atctttggcg tcgtgaatgg cgttgtgacg cacaacggcg
      961 gtacccactg caacgcggcg caggaggtct tacaatcctc actggagagt gttgagaagg
     1021 cactgaagaa ggataacaaa gtgattgaca caaatcgtgt tttgcgacat ttcatgatcc
     1081 ttgtttttct cgtgcaggtg caaccgaagt ttgattcgca gaacaaggcg cgccttgttt
     1141 ctgtgccaac gatgccgcgt gttccaagac aggaattgat ggactttctt cttcgcatgc
     1201 cgtttctcga ggcgcatgtc aacaccgtga cggggcagct cgcagatgaa cttaataagg
     1261 agatgggcgc cgggcgacgc atgagcagca aatctctcat ctcttccatc acgaaattgg
     1321 tggacgccac cacgactcga cgggacccaa ggttcgtccg caccctcatt gtcacggagg
     1381 gcgactctgc aaaggcactc gcccagaact ccctgtcaag cgaccagaaa cgctacacag
     1441 gtgtgttccc actccgtggg aagcttttaa acgtgcgcaa taaaaatctc aaacgtctta
     1501 aaaactgcaa agagctacag gagctcttct gtgccttggg gcttgaactg gggaagatct
     1561 acaaggacgc ggaagagcta cgctatcaac gcctgctagt catgacagac caggatgccg
     1621 atggatcaca catcaaagga cttgtcatca atgcctttga ggcactatgg ccctcgttgc
     1681 tgaatcgcaa tccgggcttc atttccattt tttcgacgcc cattgtaaag gtgcgtctga
     1741 gagacaagtc gacccattcc tttttcagtc tgaaggagtt tcacaagtgg cagaagacac
     1801 atggaaatgt ttcctacacc gccaagtatt acaaggctct cggtacatcc acaacagccg
     1861 aggggaagga gtactttaaa gacatggata agcacacaat gcgacttgtg gtggaacgta
     1921 atgaccataa attacttgac agtgtgtttg attcccagga agtggagtgg cgcaaggatt
     1981 ggatgacaaa ggcaaacgct tacacagggg aagttgacat agatcgaagt aagaagacac
     2041 tgaccgtacc cgattttgtt cacaaagaga tggtgcactt tgcacttgcc ggtaatgccc
     2101 gcgcccttgc acacgctgtg gatggattaa aaccctccca acgaaaaatt ttgtgggcca
     2161 ttatgcgtcg ctctggtaac gaatcggcca aagtggctca gttgtcaggt tatatttcgg
     2221 aggtttcggc ctttcaccac ggggaaatgt cattgcaaga aacgattatt aaaatggctc
     2281 aaaattttac gggtgggaat aacattaacc ttctcattcc agagggtcag tttggttctc
     2341 gtcagcagct tggtaatgat cacgcggcag ctcgttacat ctttacaaaa ctctccagtt
     2401 tggcacggat acttttcccc agtgaagatg agcctctgtt ggattacgtt acggaagaag
     2461 gacagcaggt ggagccaaac cactacgttc ccattcttcc tttgctgctg tgcaacggca
     2521 gtgttggtat tggatttggt tttgcatcaa acattcctcc ttttcatccg cttgacgttt
     2581 cagcagcggt acgaagcatg atcaatggcg aggctgccaa agttgtagta cggcggcttg
     2641 tgccgtgggc tgtaggatat cagggtgaag tacgtcgagg gcccgaagga gaatttattg
     2701 cggccggaag ttatcagtac tacgtagatg gccgtgtgca cgtgacggag ataccatgga
     2761 cacttagcat tgaggcattc cgcgaccata tttccgttct tgcttcgaag gatgttgtgc
     2821 agcgtattgc cgactattca ggtgcgaacc atgtggacat tgacttggaa cttacgaatg
     2881 gcgccatgac aacgtacgcg gagtgtgaga gtgaactttc cctcacccaa cgtatttaca
     2941 tcaatgggac tgtcttctcc cccactggtg tcctcacccc tctggaaggg gatttggcac
     3001 ctgtcttaca gtggcattac gaccgccgcc ttgaccttta caagaaacga cgtcaacgta
     3061 acttgggcct tcttgaggct gaactcgcac gggagaaatc cacgctgaag tttgtgaccc
     3121 actttcgcga aggaaaaatt gacattgtca acgccacaga cgacagtctg gcaaagactt
     3181 gtagtaaatt gggtatggtt cgcgtcgatg attcatatga ctatgtcctg cgtaaaccca
     3241 tcacctttta cacaaaaacg agcctggaga atctcaatcg caaaatcagc gaaaccgaga
     3301 aacgcatcga caagctcaag aagacagcac ctgttcagat gtggcttgac gagctcgacc
     3361 gcttcgaccg cgcatttgag gagcatgaga acacggcggt ggcgaccatt cttaaggagc
     3421 gacgggtgaa ccccccaaca ggagatgtta gtcggaacct gcagcagccg cgactggagt
     3481 tggaggaggt caaagtctcg tcttccggag ggaaaagcgt cccaatgcgg gtgagggtgc
     3541 ggaagtacgt gcccccgcca ccgagtaaac ggccccatgt aggccagagt gttggtggtg
     3601 gtggtggtgg tggcagcgtc aggagcagtg cggcggcggt ggtggcacat gtgaaggccg
     3661 aaaagaaagc ggcgcgagca agaagcatgc agaagatgtt gttagatgtc gttgcacggc
     3721 aagtggcacg cgtccttcca cgactgccgt ggtttttgtt ttgagacatg cgccaattga
     3781 aagaaagaat tgaaagaaaa aagaaaaggc gaggcatttt taaccagctt aaagtgaaaa
     3841 aaaaaaaaaa aagggagggg ggggggggag gagaagctgt gaacctagtt tgagtctggg
     3901 gatggggtgc gctcatcatt acatgataga gcatagaagt tttcctttta aatccgccgg
     3961 aatgaaaaat aaaataa
//