From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: S. thermophilum tryptophanase gene.
Date: 9 Jan 93 05:47:53 GMT
Distribution: bionet
Checksum: 60822 4

LOCUS       STHTHA2      1640 bp ds-DNA             BCT       09-JAN-1993
DEFINITION  S. thermophilum tryptophanase gene.
ACCESSION   D10013
KEYWORDS    tryptophanase.
SOURCE      Symbiobacterium thermophilum (strain IAM 13621) DNA.
  ORGANISM  Symbiobacterium thermophilum
            Prokaryotae; Bacteria.
REFERENCE   1  (bases 1 to 1640)
  AUTHORS   Hirahara,T., Suzuki,S., Horinouchi,S. and Beppu,T.
  TITLE     Cloning, nucleotide sequences, and overexpression in Escherichia
            coli of tandem copies of a tryptophanase gene in an obligately
            symbiotic thermophile, symbiobacterium thermophilum
  JOURNAL   Appl. Environ. Microbiol. 58, 2633-2642 (1992)
  STANDARD  full automatic
COMMENT     These data kindly submitted in computer redable form by: Sueharu
            Horinouchi Department of Agricultural Chemistry Faculty of
            Agriculture The University of Tokyo Bunkyou-ku Tokyo 113 Japan
            Phone:  03-3812-2111 x5147 Fax:    03-3812-0544.
FEATURES             Location/Qualifiers
     RBS             244..250
     CDS             259..1620
                     /EC_number="4.1.99.1"
                     /gene="TNA2"
                     /product="tryptophanase"
                     /codon_start=1
                     /translation="MPKGEPFKIKMVEPIRLIPREDREAAIKAAHYNPFLLRSSDVYI
                     DLLTDSGTGAMSQFQWSAMMLGDESYAGASSYYRLKEAVTDITGYEYVLPTHQGRGAE
                     KSAFAQLITRPGMYVLSNMFFDTTRGHVQLAGRPIDLLLDVPTEEYHPFKGNMDTARL
                     EAFIQEHGAENIACIVMTVTNNSAGGQPVSMANIRETSRIARKYGILLLFDVARYAEN
                     CHFIRMREEGYADKAPIDIAREMFSYGDGLMMSAKKDALVNIGGLLAFKDEELYTRVG
                     GTVVPFEGFLTYGGLAGRDLEAMAVGLREALDPDYLAYRVGQVEYLGNLLRSAGIPIQ
                     WPVGGHAVFIDAAKFLPHIPWDQFPGHALTVALYQEGGVRTVEVGSLVMGRDPETGEN
                     VRSPFEFTRLAIPRRVYTNLHLEDVAETVINAFQKREQIRGVKFTREPKVLRHFTAHF
                     DLV"
BASE COUNT      298 a    538 c    536 g    268 t
ORIGIN      
        1 aggcggggct gcgggccttc gactccaagg ccgaactggc acgccacctg gggctctcca
       61 gggccaccct ctaccggaag ctggcgcgtt acgggctggc atgatgtatc ggaataatac
      121 atagtgagac ggtgtttgtt tcatcttgat acatgtgagg gccgggagaa acctcccggc
      181 ccttctgctg tcgttggtat gatccttgct cgtaaggtcg gcggacgcgc catcatcata
      241 acgaaggagg aaccagtgat gccaaagggc gagcccttta agatcaagat ggtggaaccg
      301 atccggctga tcccccggga ggaccgggag gcggccatca aggcagcgca ctacaatccc
      361 ttcctgctcc ggtcctccga cgtctacatc gacctgctca ccgactccgg cacgggcgcg
      421 atgagccagt tccagtggtc cgccatgatg ctgggcgacg agtcgtacgc cggcgccagc
      481 agctactacc ggctgaagga ggcggtcact gacatcaccg ggtacgagta cgtcctcccg
      541 acccaccagg gccgcggcgc cgagaagagc gccttcgcgc agctgatcac ccggccgggc
      601 atgtacgtgc tctccaacat gttcttcgac acgactcgcg gccacgtgca gctggccggc
      661 cggccgatcg acctgctgct ggacgtgccc accgaggagt accacccctt caagggcaac
      721 atggacacgg ccaggctcga ggccttcatt caggagcacg gcgcggagaa catcgcctgc
      781 atcgtgatga ccgtgaccaa caactccgcg ggcgggcagc ccgtatccat ggccaacatc
      841 cgcgagacgt cccgcatcgc gcggaagtac ggcatcctgc tgctgttcga cgtggcccgg
      901 tacgccgaga actgccactt catccggatg cgggaggaag gctacgctga caaggcgccc
      961 atcgacatcg cccgggagat gttctcctac ggcgacgggt tgatgatgag cgccaagaag
     1021 gacgcgctgg tgaacatcgg cggcctgctg gccttcaagg acgaggagct gtacacccgc
     1081 gtgggcggca cggtcgtgcc cttcgagggc ttcctgacct acggcggcct ggccggccgg
     1141 gatctggagg ccatggcggt gggcctccgg gaggcgctgg atcccgacta cctggcgtac
     1201 cgggtgggcc aggtggagta cctgggcaac ctgctgcgca gcgccggcat cccgatccag
     1261 tggccggtgg gcggccatgc ggtgttcatc gacgccgcga agttcctgcc gcacatcccg
     1321 tgggaccagt tccccggcca cgcgctcacg gtggcgctct accaggaggg cggcgtgcgc
     1381 accgtggagg tcggctcgct cgtgatgggc cgcgaccccg agaccggcga gaacgtccgc
     1441 agcccgttcg agttcacccg gctggcgatt ccgcggcggg tctacaccaa cctgcacctg
     1501 gaagacgtcg ccgagaccgt gatcaacgcc ttccagaagc gggagcagat ccgcggcgtc
     1561 aagttcaccc gggagccgaa ggtgctgcgc cacttcacgg cgcacttcga tctggtctag
     1621 ctcaagtttc acgcgaacaa
//