From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Integron In7 (from Plasmid pDGO100 from Escherichia coli) integrase
Date: 21 Dec 92 20:20:22 GMT
Distribution: bionet
Checksum: 07621 20

LOCUS       INTINTORF    6747 bp ds-DNA             BCT       17-NOV-1992
DEFINITION  Integron In7 (from Plasmid pDGO100 from Escherichia coli) integrase
            (int), aminoglycoside adenylyltransferase (aad), quaternary
            ammonium compound-resistance protein, dihydrofolate reductase
            (dhfrX), and dihydropteroate synthase (sulI) genes.
ACCESSION   L06418
KEYWORDS    aad gene; aminoglycoside adenylyltransferase; dhfrX gene;
            dihydrofolate reductase; dihydropteroate synthase;
            gentamicin resistance; int gene; integrase; kanamycin resistance;
            quaternary ammonium compound-resistance protein; sulI gene;
            tobramycin resistance.
SOURCE      Integron In7 DNA.
  ORGANISM  Integron In7
            Prokaryotae.
REFERENCE   1  (bases 1 to 6747)
  AUTHORS   Cameron,F.H., Obbink,D.J., Ackerman,V.P. and Hall,R.M.
  TITLE     Nucleotide sequence of the AAD(2'') aminoglycoside
            adenylyltransferase determinant aadB. Evolutionary relationship of
            this region with those surrounding aadA in R538-1 and dhfrII in
            R388
  JOURNAL   Nucleic Acids Res. 14, 8625-8635 (1986)
  STANDARD  full automatic
REFERENCE   2  (bases 1 to 6747)
  AUTHORS   Hall,R.M. and Vockler,C.
  TITLE     The region of the IncN plasmid R46 coding for resistance to
            beta-lactam antibiotics, streptomycin/spectinomycin and
            sulphonamides is closely related to antibiotic resistance segments
            found in IncW plasmids and in Tn21-like transposons
  JOURNAL   Nucleic Acids Res. 15, 7491-7501 (1987)
  STANDARD  full automatic
REFERENCE   3  (bases 1 to 6747)
  AUTHORS   Hall,R.M. and Stokes,H.W.
  TITLE     The structure of a partial duplication in the integron of plasmid
            pDGO100
  JOURNAL   Plasmid 23, 76-79 (1990)
  STANDARD  full automatic
REFERENCE   4  (bases 1 to 6747)
  AUTHORS   Parsons,Y., Stokes,H.W. and Hall,R.M.
  TITLE     A new plasmid encoded trimethoprim resistance gene, dhfrX
  JOURNAL   Antimicrob. Agents Chemother. 35, 2436-2439 (1991)
  STANDARD  full automatic
REFERENCE   5  (bases 1 to 6747)
  AUTHORS   Stokes,H.W., Tomaras,C., Parsons,Y. and Hall,R.M.
  TITLE     The partial 3'-conserved segment duplications in the integrons In6
            from pSa and In7 from pDGO100 have a common origin
  JOURNAL   Plasmid (1993) In press
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     misc_feature    1..1285
                     /standard_name="'5'-conserved segment'"
                     /note="also found under accession number X04555"
                     /citation=[2]
                     /citation=[1]
     old_sequence    replace(294,"")
                     /note="'corrected in [5]'"
                     /note="'cg' in old_sequence"
                     /citation=[1]
     old_sequence    replace(745,"")
                     /note="'corrected in [5]'"
                     /note="'ca' in old_sequence"
                     /citation=[1]
     -35_signal      1035..1040
                     /note="'Pant, insert gene promoter'"
                     /citation=[1]
     -10_signal      1058..1063
                     /note="'Pant, insert gene promoter'"
                     /citation=[1]
     -10_signal      complement(1177..1182)
                     /gene="int"
                     /citation=[2]
     -35_signal      complement(1200..1205)
                     /gene="int"
                     /citation=[2]
     misc_feature    1286..1876
                     /function="'gene insert unit'"
                     /standard_name="'aadB cassette'"
                     /citation=[1]
     misc_feature    1824..1833
                     /note="Escherichia coli aadB 59 base pair element"
                     /evidence="EXPERIMENTAL"
                     /citation=[1]
     misc_feature    1877..3190
                     /note="'bases 1..1355 of 3'conserved segment'"
                     /note="3' conserved segment"
                     /citation=[3]
     -35_signal      1928..1933
                     /note="ORF4 promoter"
                     /gene="sulI"
     -10_signal      1952..1957
                     /note="'orf4/sulI promoter'"
                     /note="ORF4 promoter"
                     /gene="sulI"
     RBS             1975..1978
                     /note="for ORF4"
     old_sequence    replace(2109,"")
                     /note="'ga' in old_sequence"
                     /citation=[1]
     misc_feature    2229..3681
                     /note="'accession number X15369'"
                     /citation=[3]
     RBS             2314..2318
                     /gene="sulI"
                     /citation=[3]
     misc_feature    3191..5295
                     /note="'region also found in In6 from pSa'"
                     /citation=[5]
     old_sequence    replace(3214,"")
                     /note="'tt' in old_sequence"
                     /citation=[3]
     old_sequence    replace(3236,"")
                     /note="'a' in old_sequence"
                     /citation=[3]
     old_sequence    replace(3264..3265,"")
                     /note="'g' in old_sequence"
                     /citation=[3]
     -35_signal      3322..3327
                     /note="for ORF341"
                     /citation=[5]
     -10_signal      3344..3349
                     /note="for ORF341"
                     /citation=[5]
     RBS             3515..3519
                     /note="for ORF341"
                     /citation=[5]
     old_sequence    replace(3543..3544,"")
                     /note="'g' in old_sequence"
                     /citation=[3]
     old_sequence    replace(3677,"")
                     /note="'a' in old_sequence"
                     /citation=[3]
     misc_feature    4799..6747
                     /note="'accession number M69220'"
                     /citation=[4]
     old_sequence    replace(4922..4923,"")
                     /note="'g' in old_sequence"
                     /citation=[4]
     -35_signal      5396..5401
                     /gene="dhfrX"
                     /citation=[4]
     -10_signal      5419..5424
                     /gene="dhfrX"
                     /citation=[4]
     RBS             5434..5436
                     /gene="dhfrX"
                     /citation=[4]
     RBS             6034..6037
                     /citation=[4]
     misc_feature    6103..6747
                     /standard_name="'3'-conserved segment'"
                     /note="'starts at base 78 of the 3'-conserved segment'"
                     /citation=[4]
     RBS             6373..6377
                     /gene="sulI"
     CDS             complement(137..1150)
                     /note="'orf3 in [5]'"
                     /citation=[2]
                     /gene="int"
                     /product="integrase"
                     /codon_start=1
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVNWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     CDS             1296..1829
                     /note="'confers resistance to gentamicin, tobramycin and
                     kanamycin'"
                     /citation=[1]
                     /gene="aad"
                     /product="aminoglycoside adenylyltransferase"
                     /codon_start=1
                     /translation="MDTTQVTLIHKILAAADERNLPLWIGGGWAIDARLGRVTRKHDD
                     IDLTFPGERRGELEAIVEMLGGRVMEELDYGFLAEIGDELLDCEPAWWADEAYEIAEA
                     PQGSCPEAAEGVIAGRPVRCNSWEAIIWDYFYYADEVPPVDWPTKHIESYRLACTSLG
                     AEKVEVLRAAFRSRYAA"
     CDS             1986..2333
                     /note="ORF4"
                     /note="putative"
                     /product="quaternary ammonium compound-resistance protein"
                     /codon_start=1
                     /translation="MKGWLFLVIAIVGEVIATSALKSSEGFAKLAPSAVVIIGYGIAF
                     YFLSLVLKSIPVGVAYAVWSGLGVVIITAIAWLLHGQKLDAWGFVGMGLIIAAFLLAR
                     SPSWKSLRRPTPW"
     CDS             2327..3166
                     /note="'confers resistance to sulphonamides'"
                     /citation=[3]
                     /product="dihydropteroate synthase"
                     /codon_start=1
                     /translation="MVTVFGILNLTEDSFFDESRRLDPAGAVTAAIEMLRVGSDVVDV
                     GPAASHPDARPVSPADEIRRIAPLLDALSDQMHRVSIDSFQPETQRYALKRGVGYLND
                     IQGFPDPALYPDIAEADCRLVVMHSAQRDGIATRTGHLRPEDALDEIVRFFEARVSAL
                     RRSGVAADRLILDPGMGFFLSPAPETSLHVLSNLQKLKSALGLPLLVSVSRKSFLGAT
                     VGLPVKDLGPASLAAELHAIGNGADYVRTHAPGDLRSAITFSETLAKFRSRDARDRGL
                     DHA"
     CDS             3526..4551
                     /citation=[5]
                     /gene="sulI"
                     /product="ORF341"
                     /codon_start=1
                     /translation="MSLARNATASQSPTQTNGYERHQPDQTLLYQLVEQHYPAFKASL
                     EAQGQHLPRYIQQEFNDLLQCGRLEYGFMRVRCEDCHHERLVAFSCKRRGFCPSCGAR
                     RMAESAALLIDEVFPKEPIRQWVLSFPFQLRFLLARHPQLMGQVLSIVYRTLSTHLIK
                     KAGYTKASAQTGSVTLIQRFGSALNLNVHYHMLFLDGVYAEDDYGKQRFHRVKAPTYD
                     ELNTLAHTLSHRIARCMEKRGILERDAENTWLTLEEGEDDTLTQLHGASVTYRIAVGP
                     QQGRKVFTLQTLPGREDKADSSSRVANHAGFSLHAGVMAEAHQRISLSACVATLVGQR
                     FQKNVWH"
     CDS             5445..6008
                     /note="'confers resistance to trimethoprim'"
                     /citation=[4]
                     /gene="dhfrX"
                     /product="dihydrofolate reductase"
                     /codon_start=1
                     /translation="MNISLIFANELITRAFGNQGKLPWQFIKEDMQFFQKTTENSVVV
                     MGLNTWRSLPKMKKLGRDFIVISSTITEHEVLNNNIQIFKSFESFLEAFRDTTKPINV
                     IGGVGLLSEAIEHASTVYMSSIHMVKPVHADVYVPVELMNKLYSDFKYPENILWVGDP
                     IDSVYSLSIDKFVRPASLVGVPNDINT"
     CDS             6045..6392
                     /note="ORF4"
                     /note="putative"
                     /citation=[4]
                     /product="quaternary ammonium compound-resistance protein"
                     /codon_start=1
                     /translation="MKGWLFLVIAIVGEVIATSALKSSEGFTKLAPSAVVIIGYGIAF
                     YFLSLVLKSIPVGVAYAVWSGLGVVIITAIAWLLHGQKLDAWGFVGMGLIIAAFLLAR
                     SPSWKSLRRPTPW"
     CDS             6386..>6747
                     /citation=[4]
                     /product="dihydropteroate synthase"
                     /codon_start=1
                     /translation="MVTVFGILNLTEDSFFDESRRLDPAGAVTAAIEMLRVGSDVVDV
                     GPAASHPDARPVSPADEIRRIAPLLDALSDQMHRVSIDSFQPETQRYALKRGVGYLND
                     IQGFPDPALYPDIAEADC"
BASE COUNT     1566 a   1824 c   1823 g   1534 t
ORIGIN      
        1 ggatccatca ggcaacgacg ggctgctgcc ggccatcagc ggacgcaggg aggactttcc
       61 gcaaccggcc gttcgatgcg gcaccgatgg ccttcgcgca ggggtagtga atccgccagg
      121 attgacttgc gctgccctac ctctcactag tgaggggcgg cagcgcatca agcggtgagc
      181 gcactccggc accgccaact ttcagcacat gcgtgtaaat catcgtcgta gagacgtcgg
      241 aatggccgag cagatcctgc acggttcgaa tgtcgtaacc gctgcggagc aaggccgtcg
      301 cgaacgagtg gcggagggtg tgcggtgtgg cgggcttcgt gatgcctgct tgttctacgg
      361 cacgtttgaa ggcgcgctga aaggtctggt catacatgtg atggcgacgc acgacaccgc
      421 tccgtggatc ggtcgaatgc gtgtgctgcg caaaaaccca gaaccacggc caggaatgcc
      481 cggcgcgcgg atacttccgc tcaagggcgt cgggaagcgc aacgccgctg cggccctcgg
      541 cctggtcctt cagccaccat gcccgtgcac gcgacagctg ctcgcgcagg ctgggtgcca
      601 agctctcggg taacatcaag gcccgatcct tggagccctt gccctcccgc acgatgatcg
      661 tgccgtgatc gaaatccaga tccttgaccc gcagttgcaa accctcactg atccgcatgc
      721 ccgttccata cagaagctgg gcgaacaaac gatgctcgcc ttccagaaaa ccgaggatgc
      781 gaaccacttc atccggggtc agcaccaccg gcaagcgccg cgacggccga ggtcttccga
      841 tctcctgaag ccagggcaga tccgtgcaca gcaccttgcc gtagaagaac agcaaggccg
      901 ccaatgcctg acgatgcgtg gagaccgaaa ccttgcgctc gttcgccagc caggacagaa
      961 atgcctcgac ttcgctgctg cccaaggttg ccgggtgacg cacaccgtgg aaacggatga
     1021 aggcacgaac ccagttgaca taagcctgtt cggttcgtaa actgtaatgc aagtagcgta
     1081 tgcgctcacg caactggtcc agaaccttga ccgaacgcag cggtggtaac ggcgcagtgg
     1141 cggttttcat ggcttgttat gactgttttt ttgtacagtc tatgcctcgg gcatccaagc
     1201 agcaagcgcg ttacgccgtg ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc
     1261 agcagggcag tcgccctaaa acaaagttag gccgcatgga cacaacgcag gtcacattga
     1321 tacacaaaat tctagctgcg gcagatgagc gaaatctgcc gctctggatc ggtgggggct
     1381 gggcgatcga tgcacggcta gggcgtgtaa cacgcaagca cgatgatatt gatctgacgt
     1441 ttcccggcga gaggcgcggc gagctcgagg caatagttga aatgctcggc gggcgcgtca
     1501 tggaggagtt ggactatgga ttcttagcgg agatcgggga tgagttactt gactgcgaac
     1561 ctgcttggtg ggcagacgaa gcgtatgaaa tcgcggaggc tccgcagggc tcgtgcccag
     1621 aggcggctga gggcgtcatc gccgggcggc cagtccgttg taacagctgg gaggcgatca
     1681 tctgggatta cttttactat gccgatgaag taccaccagt ggactggcct acaaagcaca
     1741 tagagtccta caggctcgca tgcacctcac tcggggcgga aaaggttgag gtcttgcgtg
     1801 ccgctttcag gtcgcgatat gcggcctaac aattcgtcca agccgacgcc gcttcgcggc
     1861 gcggcttaac tcaggtgtta gatgcactaa gcacataatt gctcacagcc aaactatcag
     1921 gtcaagtctg cttttattat ttttaagcgt gcataataag ccctacacaa attgggagat
     1981 atatcatgaa aggctggctt tttcttgtta tcgcaatagt tggcgaagta atcgcaacat
     2041 ccgcattaaa atctagcgag ggctttgcta agcttgcccc ttccgccgtt gtcataatcg
     2101 gttatggcat cgcattttat tttctttctc tggttctgaa atccatccct gtcggtgttg
     2161 cttatgcagt ctggtcggga ctcggcgtcg tcataattac agccattgcc tggttgcttc
     2221 atgggcaaaa gcttgatgcg tggggctttg taggtatggg gctcataatt gctgcctttt
     2281 tgctcgcccg atccccatcg tggaagtcgc tgcggaggcc gacgccatgg tgacggtgtt
     2341 cggcattctg aatctcaccg aggactcctt cttcgatgag agccggcggc tagaccccgc
     2401 cggcgctgtc accgcggcga tcgaaatgct gcgagtcgga tcagacgtcg tggatgtcgg
     2461 accggccgcc agccatccgg acgcgaggcc tgtatcgccg gccgatgaga tcagacgtat
     2521 tgcgccgctc ttagacgccc tgtccgatca gatgcaccgt gtttcaatcg acagcttcca
     2581 accggaaacc cagcgctatg cgctcaagcg cggcgtgggc tacctgaacg atatccaagg
     2641 atttcctgac cctgcgctct atcccgatat tgctgaggcg gactgcaggc tggtggttat
     2701 gcactcagcg cagcgggatg gcatcgccac ccgcaccggt caccttcgac ccgaagacgc
     2761 gctcgacgag attgtgcggt tcttcgaggc gcgggtttcc gccttgcgac ggagcggggt
     2821 cgctgccgac cggctcatcc tcgatccggg gatgggattt ttcttgagcc ccgcaccgga
     2881 aacatcgctg cacgtgctgt cgaaccttca aaagctgaag tcggcgttgg ggcttccgct
     2941 attggtctcg gtgtcgcgga aatccttctt gggcgccacc gttggccttc ctgtaaagga
     3001 tctgggtcca gcgagccttg cggcggaact tcacgcgatc ggcaatggcg ctgactacgt
     3061 ccgcacccac gcgcctggag atctgcgaag cgcaatcacc ttctcggaaa ccctcgcgaa
     3121 atttcgcagt cgcgacgcca gagaccgagg gttagatcat gcctagcatt caccttccgg
     3181 ccgcccgcta aatatctcct tttgggttgt taataaaaca tccaataagt tgactgtgcg
     3241 tgaaaaagaa agttttgtgt gatggcgttg aagatcgcac cgttaagctc ttatgtggga
     3301 tggtgcagag ctcgacgact accgataaaa cgcaaccgcc cagtattgtg attggtggag
     3361 caatagcacc ctgaacccaa aaccttctcg ctcaaccggt agtggctgat aacaactcgt
     3421 gagggctatt gcgggttaag catttagcga tgtctagggc cagactggac gtctgaacgc
     3481 aagccgctga tactgtacat aaccacagta tcagggagga tacccatgtc gctggcaagg
     3541 aacgccacgg cgagtcaatc gcccactcaa acaaacggtt acgaacgcca ccaacccgac
     3601 cagacgctgc tctaccagct ggttgagcag cactacccag ccttcaaagc ctcactcgaa
     3661 gcccaaggtc aacacctgcc tcgctacatc caacaagaat tcaacgacct cctccaatgt
     3721 ggccgtctgg agtatggttt catgcgggtt cgctgcgagg attgtcatca cgagcgtctg
     3781 gtcgccttca gctgtaaacg acgcggcttt tgccctagct gcggtgcccg ccggatggcc
     3841 gagagtgcgg cgctgctgat agacgaagtc ttccccaagg agcccattcg ccagtgggtg
     3901 ctcagctttc ctttccagct acgctttttg ctggctcgcc atccccagct gatgggccag
     3961 gtcttgagta tcgtctatcg tacactctca actcatctga tcaaaaaagc cggttacacc
     4021 aaagcctctg cacaaactgg ctcagtgact cttatccaac gctttggctc cgcgctaaat
     4081 ctcaatgtcc actaccacat gctgtttctc gatggtgtct atgccgaaga tgactatggc
     4141 aagcaacgct tccatcgtgt caaggcaccc acttacgatg agctgaatac gctcgctcac
     4201 accctcagcc atcgcatcgc tcgctgcatg gaaaagcgtg ggattttgga gcgtgatgcc
     4261 gagaatacgt ggttgacact ggaagagggc gaagacgata cgctgactca attacatggt
     4321 gcttcggtta cgtatcgcat tgccgtcggc ccccagcaag ggcgcaaagt cttcaccctg
     4381 caaaccttgc cagggcgtga ggataaagcc gactcaagca gtcgagtagc caaccatgct
     4441 ggtttctcgc tacacgccgg tgtgatggcc gaagcgcatc agcggataag cttgagcgct
     4501 tgtgtcgcta cattagtcgg ccagcggttt cagaaaaacg tctggcatta accgccaatg
     4561 ggcaggtgcg ttacgagctc aaaactccgt accgcaatgg caccacccat gtgatcttcg
     4621 agccgctgga cttcatcgcc aaactcgctg cgttggtacc taagccgcga gtcaacctca
     4681 cacgcttcca cggcgtcttt gcaccgaaca gcaaacaccg agttcaagta acacccgcca
     4741 agcggcaaga agcccgacaa atcggaaggt ctcgatacta actggcgtga caagagtcct
     4801 gcagagcgcc accgcgcatg acctggatgc aacgcctcaa gcgagtcttc aatattgata
     4861 ttgaagtctg cgaacactgc ggcggtcacg tcaaagtgat tgccagcatc gaagatccga
     4921 aggtcattga gcagattctc aagcatctga aacagaaaac agccaaggcg aatgccgcca
     4981 agcagcgtga gctgccacca gaacgagcgc cgccactgac tcccagcctg ttcgatccat
     5041 cacagagtcg tctctttgac tgacgacccc aaatccaaca ctgctcaaca ctgccaactt
     5101 ttaaacgggg cggtggggca gtttgtatct ctcgagctat caggctagag attttaccgc
     5161 caaatcgaac cttattagag cggtttaggc tggaccggca gttaaaattg gggcttgagc
     5221 ggtaaacgag tgagggaatt tcaggtaaga tacttcggat gaggagcaaa aaggtggttt
     5281 atacttccta tacccgtaac tgcttacagt gagtttgaag aaaccagagc aaactacaaa
     5341 agtgaaggtg aatgtatcaa gaaactaata gctcaaggtc gtgagcgtat tgatattgga
     5401 accagtaatg gtacttgtta ttacattcct aatggatact acaaatgaat atatcactta
     5461 tctttgccaa tgaattaatt accagagcat tcggtaatca aggcaaatta ccttggcaat
     5521 tcattaaaga agatatgcag ttcttccaga agactacaga aaattctgta gtcgttatgg
     5581 gattaaatac atggagatct ctacctaaga tgaagaagct tggtagagac ttcattgtca
     5641 tatcttcaac tatcacagag cacgaagtgc tcaacaataa tatccaaata ttcaaatcat
     5701 ttgagagctt cttagaagca ttcagagaca caaccaaacc aatcaatgtc attggtggtg
     5761 ttggtttatt atctgaagcg atagaacatg ctagcactgt ttacatgagt tctattcata
     5821 tggttaaacc tgttcatgct gatgtgtatg taccggtaga actaatgaat aaactctata
     5881 gtgatttcaa atatccagaa aatattctat gggtaggtga tccaatagat tctgtgtata
     5941 gcttgtctat tgataagttt gttagaccag cttcgctggt tggggtgcca aatgatatta
     6001 atacgtgatt actaataagc cctacacaaa ttgggagata tatcatgaaa ggctggcttt
     6061 ttcttgttat cgcaatagtt ggcgaagtaa tcgcaacatc cgcattaaaa tctagcgagg
     6121 gctttactaa gcttgcccct tccgccgttg tcataatcgg ttatggcatc gcattttatt
     6181 ttctttctct ggttctgaaa tccatccctg tcggtgttgc ttatgcagtc tggtcgggac
     6241 tcggcgtcgt cataattaca gccattgcct ggttgcttca tgggcaaaag cttgatgcgt
     6301 ggggctttgt aggtatgggg ctcataattg ctgccttttt gctcgcccga tccccatcgt
     6361 ggaagtcgct gcggaggccg acgccatggt gacggtgttc ggcattctga atctcaccga
     6421 ggactccttc ttcgatgaga gccggcggct agaccccgcc ggcgctgtca ccgcggcgat
     6481 cgaaatgctg cgagtcggat cagacgtcgt ggatgtcgga ccggccgcca gccatccgga
     6541 cgcgaggcct gtatcgccgg ccgatgagat cagacgtatt gcgccgctct tagacgccct
     6601 gtccgatcag atgcaccgtg tttcaatcga cagcttccaa ccggaaaccc agcgctatgc
     6661 gctcaagcgc ggcgtgggct acctgaacga tatccaagga tttcctgacc ctgcgctcta
     6721 tcccgatatt gctgaggcgg actgcag
//