From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: E.coli flagellar protein (fliT) gene, 3'end, alpha-amylase (amyA)
Date: 12 Sep 92 10:02:18 GMT
Distribution: bionet
Checksum: 44524     6

LOCUS       ECOFTAA      1700 bp ds-DNA             BCT       12-SEP-1992
DEFINITION  E.coli flagellar protein (fliT) gene, 3'end, alpha-amylase (amyA)
            gene, complete cds.
ACCESSION   L01642
KEYWORDS    1,4-alpha-D-glucan glucanohydrolase; alpha-amylase; amyA gene;
            flagellar protein; fliT gene; glycogenase.
SOURCE      Escherichia coli (strain JA11) DNA.
  ORGANISM  Escherichia coli
            Prokaryotae; Gram-negative facultatively anaerobic rods;
            Enterobacteriaceae.
REFERENCE   1  (bases 1 to 1700)
  AUTHORS   Kawagishi,I., Mueller,V., Williams,A.W., Irikura,V.M. and
            Macnab,R.M.
  TITLE     Subdivision of flagellar region III of Escherichia coli and
            Salmonella typhimurium and identification of two additional genes
  JOURNAL   Unpublished (1992)
  STANDARD  full staff_entry
REFERENCE   2  (bases 1 to 1700)
  AUTHORS   Raha,M., Kawagishi,I., Mueller,V., Kihara,M. and Macnab,R.M.
  TITLE     Escherichia coli produces a cytoplasmic alpha-amylase, AmyA
  JOURNAL   J. Bacteriol. (1992) In press
  STANDARD  full staff_review
REFERENCE   3  (bases 1 to 1700)
  AUTHORS   Kawagishi,I., Mueller,V., Williams,A.W., Irikura,V.M. and
            Macnab,R.M.
  TITLE     Subdivision of flagellar region III of the Escherichia coli and
            Salmonella typhimurium chromosomes and identification of two
            additional genes
  JOURNAL   J. Gen. Microbiol. 138, 1051-1065 (1992)
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     CDS             <1..23
                     /number=1
                     /function="unknown"
                     /citation=1
                     /product="flagellar protein"
                     /gene="fliT"
                     /evidence=EXPERIMENTAL
                     /codon_start=3
                     /translation="PQDNLF"
                     /citation=[3]
                     /citation=[2]
     CDS             101..1588
                     /number=2
                     /EC_number="3.2.1.1"
                     /note="This gene lies in the intervening region between
                     flagellar regions IIIa and IIIb"
                     /product="alpha-amylase"
                     /gene="amyA"
                     /evidence=EXPERIMENTAL
                     /codon_start=1
                     /translation="MRNPTLLQCFHWYYPEGGSSWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGVDEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGEYSQFIWDFKCF
                     SGIDHIENPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRIDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEFPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQERVVTDENGEATFFCNGGSVSVWVIEEVI"
                     /citation=[3]
                     /citation=[2]
BASE COUNT      423 a    391 c    466 g    420 t
ORIGIN
        1 ctccgcagga taacctcttt tgaatctgaa tgagtcgatg gctcgcgaat aatccgatta
       61 cggctacgct tctaatgttc cccttgaatg gagtcgaaga atgcgtaatc ccacgctgtt
      121 acaatgtttt cactggtatt acccggaagg cggtagctct tggcctgaac tggccgagcg
      181 cgccgacggt tttaatgata ttggtatcaa tatggtctgg ttgccgcccg cctataaagg
      241 cgcatcgggc gggtattcgg tcggctacga ctcctatgat ttatttgatt taggcgagtt
      301 tgatcagaaa ggcagcatcc ctactaaata tggcgataaa gcacaactgc tggccgccat
      361 tgatgctctg aaacgtaatg acattgcggt gctgttggat gtggtagtca accacaaaat
      421 gggcgtggat gaaaaagaag ctattcgcgt gcagcgtgta aatgctgatg accgtacgca
      481 aattgacgaa gaaatcattg agtgtgaagg ctggacgcgt tacaccttcc ccgcccgtgc
      541 cggcgaatac tcgcagttta tctgggattt caaatgtttt agcggtatcg accatatcga
      601 aaaccctgac gaagatggca tttttaaaat tgttaacgac tacaccggcg aaggctggaa
      661 cgatcaggtt gatgatgaat taggtaattt cgattatctg atgggcgaga atatcgattt
      721 tcgcaatcat gccgtgacgg aagagattaa atactgggcg cgctgggtga tggaacaaac
      781 gcaatgcgac ggttttcgta ttgatgcggt caaacatatt ccagcctggt tttataaaga
      841 gtggatcgaa cacgtacagg aagttgcgcc aaagccgctg tttattgtgg cggagtactg
      901 gtcgcatgaa gttgataagc tgcaaacgta tattgatcag gtggaaggca aaaccatgct
      961 gtttgatgcg ccgctgcaga tgaaattcca tgaagcatcg cgcatggggc gcgactacga
     1021 catgacgcag attttcacgg gtacattagt ggaagccgat cctttccacg ccgtgacgct
     1081 cgttgccaat cacgacaccc aaccgttgca agccctcgaa gcgccggtcg aaccgtggtt
     1141 taaaccgctg gcgtatgcct taattttgtt gcgggaaaat ggcgttcctt cggtattcta
     1201 tccggacctc tacggtgcgc attacgaaga tgtcggtggt gacgggcaaa cctatccgat
     1261 agatatgcca ataatcgaac agcttgatga gttaattctc gcccgtcagc gtttcgccca
     1321 cggtgtacag acgttatttt tcgaccatcc gaactgcatt gcctttagcc gcagtggcac
     1381 cgacgaattt cccggctgcg tggtggtcat gtcgaacggg gatgatggcg aaaaaaccat
     1441 tcatctggga gagaattacg gcaataaaac ctggcgtgat tttctgggga accggcaaga
     1501 gagagtagtg accgacgaaa acggcgaagc aaccttcttt tgcaacggcg gcagcgtcag
     1561 cgtgtgggtt atcgaagagg tgatttaaat tcatccccgg cggcaagccg gggagatttc
     1621 attacggcag tggcgtcggt aacggcgctt tatccagcgc ggcggcacac tctgctgtcg
     1681 ggcgatccac acgctccatc
//