HDTenEightyP/NetNews huggingface dataset:
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human chromosome 4 sequence-tagged site STS4-484
Date: 21 Aug 92 10:10:47 GMT
Distribution: bionet
Checksum: 14553     3

LOCUS       HUM4STS484    400 bp ds-DNA             PRI       21-AUG-1992
DEFINITION  Human chromosome 4 sequence-tagged site STS4-484
ACCESSION   L00882
KEYWORDS    STS; human chromosome 4; sequence tagged site.
SOURCE      Homo sapiens, clone p4-605 from a chromosome 4 plasmid library;
            vector pBluescript II KS + (Stratagene)
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 400)
  AUTHORS   Goold,R.D., diSibio,G., Dugaiczyk,A., Smith,K.A., Xu,H.,
            Magrane,G., Lang,D.B., Cox,D.R., Masters,S.B. and Myers,R.M.
  TITLE     Sequence-Tagged Sites for Human Chromosome 4
  JOURNAL   Unpublished (1992) see COMMENT for author address
  STANDARD  full automatic
COMMENT     PCR components: 25 ng of human genomic DNA, 10 pmol of each
            oligonucleotide, 200 micro-M dNTPs, 0.25 U Taq  polymerase (Cetus)
            in 10 micro-l of 50 mM KCl-20 mM  Tris-HCl, pH 8.3 (at room temp),
            2.5 mM MgCl-2.  Initial denaturation at 94degC for 1.5 min, then 30
            cycles of  94degC for 15 sec, 62degC for 23 sec, and 72degC for 30
            sec, followed by a final extension at 72degC for 3.5 min, using a
            Perkin-Elmer 9600 thermocycler. PCR-amplified product size 214 bp.
            Sequence submitted by:
            Human Genome Mapping Center
            Box 0925
            University of California San Francisco
            San Francisco, CA 94143-0925  USA
            Phone: (415) 502-1612   Fax: (415) 476-8391
            e-mail: hgmcprobes@cgl.ucsf.edu
FEATURES             Location/Qualifiers
     misc_feature    2..26
                     /label=Primer1
     misc_feature    complement(191..215)
                     /label=Primer2
BASE COUNT      106 a     77 c     98 g    114 t      5 others
ORIGIN
        1 gatcacttgg caaattgttc attaggcaaa tgggtcattt agtgaaaagg tcatttagta
       61 aatcggcttt caacgacttg ccctatgaga tttctctaga gtgagcattt tcttgggtta
      121 aatgtacatt tcatttccat aatgaatact cactggcggc tagatgggaa acttcccagg
      181 gaaccaggag caaccagaac aacctcttaa aaatcaggca attaanancc ctctggtttc
      241 ttaacaggag cggaggtaca gatggcatct gctctgcaag gtgcagtgat cgggttcagc
      301 attgtcacag gagctcttgc ggttctgttt gttgntagtg tgacatacac aggcattcat
      361 cggaaaaagg actgagtggg cttnggntct cctgtagttg
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: H.sapiens IG VH mRNA (clone mAB 63VH)
Date: 14 Aug 92 10:00:48 GMT
Distribution: bionet
Checksum: 20178     3

LOCUS       HUMVHMAB63    422 bp ss-mRNA            PRI       14-AUG-1992
DEFINITION  H.sapiens IG VH mRNA (clone mAB 63VH)
ACCESSION   X54441
KEYWORDS    IgM; VH gene family IV; autoantibody; diversity exon;
            immunoglobulin heavy chain; joining exon; monoclonal antibody;
            polyreactive; rheumatoid factor; variable region.
SOURCE      Homo sapiens RNA.
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 422)
  AUTHORS   Harindranath,N., Goldfarb,I.S., Ikematsu,H., Burastero,S.E.,
            Wilder,R.L., Notkins,A.L. and Casali,P.
  TITLE     Complete sequence of the genes encoding the VH and VL regions of
            low-high affinity monoclonal IgM and IgA1 rheumatoid factors
            produced by CD5+ B cells from a rheumatoid arthritis patient
  JOURNAL   Int. Immunol. 3, 865-875 (1991)
  STANDARD  full staff_entry
COMMENT     *source: stage=adult; *source: cell_type=B-cell heterohybridoma;
            *source: cell_line=Ab 63; *source: clone_library=lambda gt11;
            *source: clone=mAB 63 VH; *source: isolate=rheumatoid arthritis
            patient (ML); *source: chromosome=14q32; *source: is_germline=N;
            See also X54435-X54438 & X54441-47
            From EMBL    entry HSVHMAB63;  dated 29-JAN-1992.
FEATURES             Location/Qualifiers
     CDS             <1..>422
                     /product="Ig heavy chain precursor"
                     /gene="IGH@"
                     /codon_start=3
                     /translation="FFLLLVAAPRWVLAQVQLQQWGAGLLKPSETLSLTCAVYGGSFS
                     GYYWSWIRQPPGKGLEWIGEINHSGSTNYNPSLKSRVTISVDTSKNQFSLKLSSVTAA
                     DTAVYYCARGGSVLRFLEWLLYPAFDYWGQGTLVTVSS"
     sig_peptide     <1..44
                     /gene="IGH@"
                     /codon_start=1
     mat_peptide     45..>422
                     /product="immunoglobulin heavy chain V-D-J region"
                     /gene="IGH@"
                     /codon_start=1
     misc_feature    355..356
                     /note="V-region end/D-region start"
                     /gene="IGH@"
     misc_feature    380..381
                     /note="D-region end/J-region start"
                     /gene="IGH@"
BASE COUNT       83 a    117 c    124 g     98 t
ORIGIN
        1 ggttcttcct cctcctggtg gcagctccca gatgggtcct ggcccaggtg cagctacagc
       61 agtggggcgc aggactgttg aagccttcgg agaccctgtc cctcacctgc gctgtctatg
      121 gtgggtcctt cagtggttac tactggagct ggatccgcca gcccccaggg aaggggctgg
      181 agtggattgg ggaaatcaat catagtggaa gcaccaacta caacccgtcc ctcaagagtc
      241 gagtcaccat atcagtagac acgtccaaga accagttctc cctgaagctg agctctgtga
      301 ccgccgcgga cacggctgtg tattactgtg cgagaggggg atcggtatta cgatttttgg
      361 agtggttatt atatcccgct tttgactact ggggccaggg aaccctggtc accgtctcct
      421 ca
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human chromosome 4 sequence-tagged site STS4-384
Date: 21 Aug 92 10:09:42 GMT
Distribution: bionet
Checksum: 11453     3

LOCUS       HUM4STS384    631 bp ds-DNA             PRI       21-AUG-1992
DEFINITION  Human chromosome 4 sequence-tagged site STS4-384
ACCESSION   L00830
KEYWORDS    STS; human chromosome 4; sequence tagged site.
SOURCE      Homo sapiens, clone p4-367 from a chromosome 4 plasmid library;
            vector pBluescript II KS + (Stratagene)
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 631)
  AUTHORS   Goold,R.D., diSibio,G., Dugaiczyk,A., Smith,K.A., Xu,H.,
            Magrane,G., Lang,D.B., Cox,D.R., Masters,S.B. and Myers,R.M.
  TITLE     Sequence-Tagged Sites for Human Chromosome 4
  JOURNAL   Unpublished (1992) see COMMENT for author address
  STANDARD  full automatic
COMMENT     PCR components: 25 ng of human genomic DNA, 10 pmol of each
            oligonucleotide, 200 micro-M dNTPs, 0.25 U Taq  polymerase (Cetus)
            in 10 micro-l of 50 mM KCl-20 mM  Tris-HCl, pH 8.3 (at room temp),
            2.5 mM MgCl-2.  Initial denaturation at 94degC for 1.5 min, then 30
            cycles of  94degC for 15 sec, 64degC for 23 sec, and 72degC for 30
            sec, followed by a final extension at 72degC for 3.5 min, using a
            Perkin-Elmer 9600 thermocycler. PCR-amplified product size 176 bp.
            STS sequence contains a simple sequence tandem repeat (CA).
            Sequence submitted by:
            Human Genome Mapping Center
            Box 0925
            University of California San Francisco
            San Francisco, CA 94143-0925  USA
            Phone: (415) 502-1612   Fax: (415) 476-8391
            e-mail: hgmcprobes@cgl.ucsf.edu
FEATURES             Location/Qualifiers
     misc_feature    240..263
                     /label=Primer1
     misc_feature    complement(391..415)
                     /label=Primer2
BASE COUNT      166 a    102 c    122 g    230 t     11 others
ORIGIN
        1 gatcaggtgt aagtggcaga gtgcttgttt tatatctttc tctctttcag tgtaaaagtt
       61 aagaataata tttcttccaa atgaaattac tgtgcctccc ttcagtcatg tgaaatataa
      121 actttaccac tgaggtttat attaactttg aaagttttta ttgttttatt tgagaaagtt
      181 ttcaagggtc atatatttta gggggtgggg tggtaggaaa aacattattt aataaaatgc
      241 gtacctgcta ttcacttacg tgtaaatgaa atgacttgtc tattttttcc tttcctttct
      301 ttgtnctaaa cccaagctaa agtaaccccc cnacantgtg tatgtgtgtg tgtgtgtgtg
      361 tgtgtgtgtg tgtgtgtgtg tgtgtgtntt gagatggttg caatattgtg ctttcttcct
      421 tcanccnaag ctgnaanaca cagcaataaa aatacttttg acagcttctg aacctaaaaa
      481 tatgagggct taatttatat tttacactgt aaatttaaga gtgactcaga cctcaggact
      541 tggcccagtt agagtttctg tgtacctatt ctctctaaat tagctcttac aatgccatgg
      601 cttggnanac canttatcag ctgatgagcc c
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Phascolosorex dorsalis cytochrome b gene, partial cds.
Date: 20 Aug 92 10:04:11 GMT
Distribution: bionet
Checksum: 54395     3

LOCUS       PCXCYTOB      641 bp ds-DNA             ORG       20-AUG-1992
DEFINITION  Phascolosorex dorsalis cytochrome b gene, partial cds.
ACCESSION   M99462
KEYWORDS    cytochrome b.
SOURCE      Mitochondrion Phascolosorex dorsalis (chromosome Mitochondrion
            Phascolosorex dorsalis) DNA.
  ORGANISM  Mitochondrion Phascolosorex dorsalis
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Metatheria; Marsupialia; Marsupicarnivora; Dasyuridae;
            Phascogalinae.
REFERENCE   1  (bases 1 to 641)
  AUTHORS   Krajewski,C., Driskell,A.C., Baverstock,P.R. and Braun,M.J.
  TITLE     Phylogenetic relationships of the thylacine (Marsupialia:
            Thylacinidae) among dasyuroid marsupials: evidence from
            cytochrome-b DNA sequences
  JOURNAL   Proc. R. Soc. Lond., B, Biol. Sci. (1992) In press
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     CDS             1..641
                     /product="cytochrome b"
                     /note="putative"
                     /codon_start=2
                     /translation="FGSLLGMCLIIQILTGLFLAMHYTSDTLTAFTSVAHICRDVNYG
                     WLLRNLHANGASMFFMCLFLHIGRGIYYGSYLYKETWNIGVILLLAVMATAFVGYVLP
                     WGQMSFWGTTVITNLLSAIPYIGPTLAEWVWGGYAVDKATLTRFFAFHFILPFIVTAL
                     AIVHLLFLHETGSNNPSGINPNADKIPFHPYYTIKDALGFMLLLSXLXXXXLF"
                     /citation=[1]
                     /partial
BASE COUNT      169 a    196 c     86 g    179 t     11 others
ORIGIN
        1 cttcggctct ttactaggaa tatgcttaat catccaaatc ctcacaggac tattcctagc
       61 aatacactac acctcagata cactcactgc cttcacctcc gtagcccaca tctgccgtga
      121 tgttaactac ggttgactac ttcgcaattt acatgctaac ggagcttcaa tattcttcat
      181 atgccttttc ctacatatcg gccgaggaat ttactatgga tcctacctct acaaagaaac
      241 atgaaacatt ggcgtaatcc tactactcgc cgtcatagct acagcatttg taggctatgt
      301 tcttccttga ggccaaatat cattttgagg cactaccgta atcactaatc tcctatccgc
      361 catcccttac atcggcccta ccttagcaga atgagtatga ggcggctacg cagtagacaa
      421 agcaacactc acacgattct ttgcctttca ctttatcctg cccttcatcg tcacagcact
      481 agccattgtt cacctactat tcctccatga aacaggctct aacaacccat caggaattaa
      541 ccccaacgca gacaaaattc cattccaccc ttactacact atcaaagacg ccctaggctt
      601 catactactc ctttccnnnc tccnnntnnn nncccttttt c
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Mus musculus IgK chain mRNA V-region, from hybridoma M195.
Date: 14 Aug 92 10:00:59 GMT
Distribution: bionet
Checksum: 35284     3

LOCUS       MUSIGKM195    393 bp ss-mRNA            ROD       14-AUG-1992
DEFINITION  Mus musculus IgK chain mRNA V-region, from hybridoma M195.
ACCESSION   M83099
KEYWORDS    immunoglobulin; immunoglobulin kappa chain; variable region.
SOURCE      Mus musculus hybridoma M195 cDNA to mRNA.
  ORGANISM  Mus musculus
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Rodentia; Myomorpha; Muridae; Murinae.
REFERENCE   1  (bases 1 to 393)
  AUTHORS   Co,M., Avdalovic,N., Caron,P.C., Avdalovic,M.V., Scheinberg,D.A.
            and Queen,C.
  TITLE     Chimeric and humanized antibodies with specificity for the CD33
            antigen
  JOURNAL   J. Immunol. 148, 1149-1154 (1992)
  STANDARD  full staff_entry
FEATURES             Location/Qualifiers
     sig_peptide     1..60
                     /gene="IgK"
                     /note="putative"
                     /codon_start=1
     CDS             1..393
                     /note="V-region"
                     /product="immunoglobulin kappa chain"
                     /gene="IgK"
                     /note="putative"
                     /codon_start=1
                     /translation="MEKDTLLLWVLLLWVPGSTGDIVLTQSPASLAVSLGQRATISCR
                     ASESVDNYGISFMNWFQQKPGQPPKLLIYAASNQGSGVPARFSGSGSGTDFSLNIHPM
                     EEDDTAMYFCQQSKEVPWTFGGGTKLEIK"
                     /partial
     mat_peptide     61..393
                     /note="V-region"
                     /product="immunoglobulin kappa chain"
                     /gene="IgK"
                     /codon_start=1
                     /partial
BASE COUNT       97 a     99 c    102 g     95 t
ORIGIN
        1 atggagaaag acacactcct gctatgggtc ctgcttctct gggttccagg ttccacaggt
       61 gacattgtgc tgacccaatc tccagcttct ttggctgtgt ctctagggca gagggccacc
      121 atctcctgca gagccagcga aagtgttgat aattatggca ttagttttat gaactggttc
      181 caacagaaac caggacagcc acccaaactc ctcatctatg ctgcatccaa ccaaggatcc
      241 ggggtccctg ccaggtttag tggcagtggg tctgggacag acttcagcct caacatccat
      301 cctatggagg aggatgatac tgcaatgtat ttctgtcagc aaagtaagga ggttccgtgg
      361 acgttcggtg gaggcaccaa gctggaaatc aaa
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Cucumber Mosaic Virus coat protein (CP) RNA, complete cds.
Date: 13 Aug 92 10:00:41 GMT
Distribution: bionet
Checksum: 22592     3

LOCUS       MCVCPAC       657 bp ss-RNA             VRL       13-AUG-1992
DEFINITION  Cucumber Mosaic Virus coat protein (CP) RNA, complete cds.
ACCESSION   M98501
KEYWORDS    coat protein.
SOURCE      Cucumber mosaic virus (individual_isolate PR50) cDNA to genomic
            RNA.
  ORGANISM  Cucumber mosaic virus
            Viridae; ss-RNA nonenveloped viruses; Isometric ss-RNA viruses;
            Cucumovirus.
REFERENCE   1  (bases 1 to 657)
  AUTHORS   Pappu,S.S., Pappu,H.R., Niblett,C.L. and Bird,J.
  TITLE     The nucleotide and the deduced amino acid sequences of coat protein
            genes of three Puerto Rican isolates of cucumber mosaic virus
  JOURNAL   Unpublished (1992)
  STANDARD  simple staff_entry
FEATURES             Location/Qualifiers
     CDS             1..657
                     /product="coat protein"
                     /gene="CP"
                     /codon_start=1
                     /translation="MDKSESTSAGRNRRRRPRRGSRSASSSADANFRVLSQQLSRLNK
                     TLAAGRPTINHPTFVGSERCKPGYTFTSITLKPPKIDRGSYYGKRLLLPDSVTEYDKK
                     LVSRIQIRVNPLPKFDSTVWVTVRKVPASSDLSVAAISAMFADGASPVLVYQYAASGV
                     QANNKLLYDLSAMRADIGDMRKYAVLVYSKDDALETDELVLHVDIEHQRIPTSGVLPV
                     "
BASE COUNT      153 a    171 c    156 g    177 t
ORIGIN
        1 atggacaaat ctgaatcaac cagtgctggt cgtaaccgtc ggcgtcgtcc gcgtcgtggt
       61 tcccgctccg cctcctcctc cgcggatgct aactttagag tcttgtcgca gcagctttcg
      121 cgacttaaca agacgttagc agctggtcgt ccaactatta accacccaac ctttgtaggg
      181 agtgaacgct gtaaacctgg gtacacgttc acatctatta ccctaaagcc gccaaaaata
      241 gaccgtgggt cttattacgg taaaaggttg ttattacctg attcagtcac ggaatatgat
      301 aagaagcttg tttcgcgcat tcaaattcga gttaatcctt tgccgaaatt tgattctacc
      361 gtgtgggtga cagttcgtaa agtccctgcc tcctcggact tatccgttgc cgccatctct
      421 gctatgttcg cggacggagc ctcaccggta ctggtttatc agtatgctgc atctggagtc
      481 caagctaaca acaaattgtt gtatgatctt tcggcgatgc gcgctgatat aggtgacatg
      541 agaaagtacg ccgtcctcgt gtattcaaaa gacgatgcgc tcgagacgga cgagttagta
      601 cttcatgttg acatcgagca ccaacgcatt cccacgtctg gggtgctccc agtctga
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Rhesus macaque polyoma virus large T antigen gene, 3' end.
Date: 22 Aug 92 10:01:37 GMT
Distribution: bionet
Checksum: 40788     3

LOCUS       SV4LTA3       334 bp ds-DNA             VRL       22-AUG-1992
DEFINITION  Rhesus macaque polyoma virus large T antigen gene, 3' end.
ACCESSION   M99356
KEYWORDS    large T antigen.
SOURCE      Rhesus macaque polyomavirus (strain Kid155) DNA.
  ORGANISM  Rhesus macaque polyomavirus
            Viridae; ds-DNA nonenveloped viruses; Papovaviridae;
            Polyomaviruses.
REFERENCE   1  (sites)
  AUTHORS   van Heuverswyn,H. and Fiers,W.
  TITLE     Nucleotide sequence of the Hind-C fragment of Simian Virus 40 DNA:
            comparison of the 5'-untranslated region of wild-type virus and of
            some deletion mutants
  JOURNAL   Eur. J. Biochem. 100, 51-60 (1979)
  STANDARD  full staff_review
REFERENCE   2  (bases 1 to 334)
  AUTHORS   Ilyinskii,P.O., Daniel,M.D., Horvath,C. and Desrosiers,R.C.
  TITLE     Genetic analysis of SV40 from brain and kidney of macaque monkeys
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     CDS             complement(16..334)
                     /note="partial"
                     /product="large T antigen"
                     /codon_start=2
                     /translation="KEFSLSVYQKMKFNVAMGTGVLDWLRNSDNDDEDSQENADKNED
                     GGEKNMEDSGHETGIDSQSQGSFQAPQPSQSSQSVHDQPYHICRGFTCFKKPPTPPPE
                     PET"
                     /partial
     variation       replace(82,"g")
                     /note="nucleotide 2757 in SV40 strain 776"
                     /citation=[1]
     mutation        replace(88^89,"tgatta")
                     /note="nucleotides 2764-2769 in SV40 strain 776"
                     /citation=[1]
     mutation        replace(100..110,"ag")
                     /note="absent in SV40 strain 776"
                     /citation=[1]
     mutation        replace(120,"a")
                     /note="nucleotide 2792 in SV40 strain 776"
                     /citation=[1]
     variation       replace(145,"g")
                     /note="nucleotide 2817 in SV40 strain 776"
                     /citation=[1]
     variation       replace(246,"c")
                     /note="nucleotide 2918 in SV40 strain 776"
                     /citation=[1]
     variation       replace(253,"g")
                     /note="nucleotide 2925 in SV40 strain 776"
                     /citation=[1]
     variation       replace(278,"a")
                     /note="nucleotide 2950 in SV40 strain 776"
                     /citation=[1]
BASE COUNT       86 a     73 c     63 g    112 t
ORIGIN
        1 acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt ttttaaagca
       61 agtaaaacct ctacaaatgt gatatggctg atcatgaaca gactgtgagg actgtgaggg
      121 ctgaggggcc tgaaatgagc cttgagactg tgaatcaatg cctgtttcat gccctgagtc
      181 ttccatgttc ttctccccac catcttcatt tttatcagca ttttcctggc tgtcttcatc
      241 atcattatca ctatttctta gccaatctaa aactccagtt cccatagcca cattaaactt
      301 cattttttga tacactgaca aactaaactc tttg
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human chromosome 4 sequence-tagged site STS4-139
Date: 21 Aug 92 10:06:54 GMT
Distribution: bionet
Checksum: 56217     3

LOCUS       HUM4STS139    375 bp ds-DNA             PRI       21-AUG-1992
DEFINITION  Human chromosome 4 sequence-tagged site STS4-139
ACCESSION   M95266
KEYWORDS    STS; human chromosome 4; sequence tagged site.
SOURCE      Homo sapiens, clone C4-247 from Los Alamos National Laboratory
            chromosome 4 cosmid library, plate 4-1-2R, pos 7A; vector SuperCos
            1 (Stratagene)
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 375)
  AUTHORS   Goold,R.D., diSibio,G., Dugaiczyk,A., Smith,K.A., Xu,H.,
            Magrane,G., Lang,D.B., Cox,D.R., Masters,S.B. and Myers,R.M.
  TITLE     Sequence-Tagged Sites for Human Chromosome 4
  JOURNAL   Unpublished (1992) see COMMENT for author address
  STANDARD  full automatic
COMMENT     PCR components: 25 ng of human genomic DNA, 10 pmol of each
            oligonucleotide, 200 micro-M dNTPs, 0.25 U Taq  polymerase (Cetus)
            in 10 micro-l of 50 mM KCl-20 mM  Tris-HCl, pH 8.3 (at room temp),
            2.5 mM MgCl-2.  Initial denaturation at 94degC for 1.5 min, then 30
            cycles of  94degC for 15 sec, 62degC for 23 sec, and 72degC for 30
            sec, followed by a final extension at 72degC for 3.5 min, using a
            Perkin-Elmer 9600 thermocycler. PCR-amplified product size 239 bp.
            Sequence submitted by:
            Human Genome Mapping Center
            Box 0925
            University of California San Francisco
            San Francisco, CA 94143-0925  USA
            Phone: (415) 502-1612   Fax: (415) 476-8391
            e-mail: hgmcprobes@cgl.ucsf.edu
FEATURES             Location/Qualifiers
     misc_feature    16..38
                     /label=Primer1
     misc_feature    complement(232..254)
                     /label=Primer2
BASE COUNT      127 a     70 c     84 g     90 t      4 others
ORIGIN
        1 aagggttccg tagtacatcc cttcatacta ccatgccatg tgattaacgt caatggaaaa
       61 ctacaacaac ccaatgcaaa cagtactcta tgggtccaga cccttaagga acaaaggatt
      121 ggactggact gtgttcttca aagagacatg ttcgagtcct aattcgcact atctgtgaat
      181 gtgaccttat ttngaaatag ggtctttgca gatgaaatca agttaagata aggttattct
      241 ggattagggt gaggcctaat gtaataactg ctattctnac aagaacagag aaacttgaac
      301 acagacccag aggaaacaca cagagagaag gagaacacta tgtaatggta gaggcagnga
      361 tgnacgtgat gcatc
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Ganoderma lucidum ribosomal RNA (59LR0R) large subunit, partial.
Date: 18 Aug 92 10:00:52 GMT
Distribution: bionet
Checksum: 51599     2

LOCUS       GANRRLS       225 bp ss-rRNA            RNA       18-AUG-1992
DEFINITION  Ganoderma lucidum ribosomal RNA (59LR0R) large subunit, partial.
ACCESSION   M98667
KEYWORDS    large subunit ribosomal RNA.
SOURCE      Ganoderma lucidum (library: Duke culture collection 780) cDNA to
            rRNA.
  ORGANISM  Ganoderma lucidum
            Eukaryota; Fungi; Eumycota; Basidiomycotina; Hymenomycetes;
            Agaricales.
REFERENCE   1  (bases 1 to 225)
  AUTHORS   Hibbett,D.S. and Vilgalys,R.
  TITLE     Phylogenetic relationships of the basidiomycete genus Lentinus
            inferred from molecular and morphological characters
  JOURNAL   Unpublished (1992)
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     rRNA            <1..>225
                     /note="Aligns to region between positions 70 to 306
                     inSaccharomyces cerevisiae large subunit nuclear rRNA."
                     /product="large subunit ribosomal RNA"
                     /citation=[1]
BASE COUNT       55 a     40 c     72 g     58 t
ORIGIN
        1 taggtaaggc gagtgaaggg gaaaagctca aatttaaaat ctggcggtct ttggccgtcc
       61 gagttgtagt ctggagaagt gcttccgacg ctggaccgtg tataagtctc ttggaacaga
      121 gcgtcataga gggtgagaat ccgtctttga cacggactac cagtgctttg tgatgcgctc
      181 tcaaagagtc gagttgtttg ggaatgcagc tcaaaatggg tggtg
//