From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: pcnB=plasmid copy number control gene,
Date: 6 Jan 93 07:09:47 GMT
Distribution: bionet
Checksum: 14945 4

LOCUS       S113052      1012 bp    DNA             BCT       04-DEC-1992
DEFINITION  pcnB=plasmid copy number control gene,
            folK=7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase
            [Escherichia coli, Genomic, 1012 nt]
ACCESSION   S44849
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Unclassified.
REFERENCE   1  (bases 1 to 1012)
  AUTHORS   Talarico,T.L., Ray,P.H., Dev,I.K., Merrill,B.M. and Dallas,W.S.
  TITLE     Cloning, sequence analysis, and overexpression of Escherichia coli
            folK, the gene coding for
            7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase.
  JOURNAL   J. Bacteriol. 174, 5971-5977 (1992)
  STANDARD  full automatic
COMMENT     This entry [NCBI gibbsq 113052] was created by the journal scanning
            component of NCBI/GenBank at the National Library of Medicine.
            This sequence comes from Fig. 2.
FEATURES             Location/Qualifiers
     CDS             2..124
                     /partial
                     /note="Description: plasmid copy number control gene"
                     /gene="pcnB"
     CDS             133..612
                     /note="HPPK;  For the protein sequence (NCBI gibbsq
                     113055):  Method: conceptual translation with partial
                     peptide sequencing.  This sequence comes from Fig. 2."
                     /gene="folK"
                     /product="7,
                     8-dihydro-6-hydroxymethylpterin-pyrophosphokinase"
                     /codon_start=1
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESHILTVSSFYRTP
                     PLGPQDQPDYLNAAVALETSLAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELVFPDGEMLRQILHTRAFDKLNKW"
BASE COUNT      295 a    233 c    197 g    287 t
ORIGIN      
        1 atggtggggt gagttccagg tttccgcgcc accagaccaa aaagggatcg tcaacgagct
       61 ggatgaagaa ccgtcaccgc gtcagtcgta ctcgtcgtcc acgcaaacgc gcaccacgtc
      121 gtagggtacc gcatgacagt ggcgtatatt gccataggca gcaatctggc ctctccgctg
      181 gagcaggtca atgctgccct gaaagcatta ggcgatatcc ctgaaagcca cattcttacc
      241 gtttcttcgt tttaccgcac cccaccgctg ggcccgcaag atcaacccga ttacttaaac
      301 gcagccgtgg cgctggaaac ctctcttgca cctgaagagc tactcaatca cacacagcgt
      361 attgaattgc agcaaggtcg cgtccgcaaa gctgaacgct ggggaccacg cacgctggat
      421 ctcgacatca tgctgtttgg taatgaagtg ataaatactg aacgcctgac cgttccgcac
      481 tacgatatga agaatcgtgg atttatgctg tggccgctgt ttgaaatcgc gccggagttg
      541 gtgtttcctg atggggagat gttgcgtcaa atcttacata caagagcatt tgacaaatta
      601 aacaaatggt aatttattta tattgcgaat ataaatattt tctgaaaatc atatctcatc
      661 tctttgcctt aattacgatt ttagtttaaa catctctttg cggaaataat ataacttcct
      721 ataaataaat atcaatccca tctccatacc ctaaccaatt gcacttgtga acttattttt
      781 acttaatttt gaattaagta aatttaattt gttcaaaagc ttctaaagtg cacaataggc
      841 gacctaatga acaatggttg taaatttggg taacaagtaa tccagcggat aacacgtgaa
      901 atcttcgaaa acttgtttaa tttaaatgaa ttaagtttta attcattttt attcaagtgt
      961 tcacgttaac caatcccata cctctaccct aactataaat aaatatcctt ca
//