From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Drosophila melanogaster (lambda J2 clone, pBSr-1Eco subclone) UMP
Date: 3 Sep 92 10:00:36 GMT
Distribution: bionet
Checksum: 21662     9

LOCUS       DROUMPS      3710 bp ds-DNA             INV       03-SEP-1992
DEFINITION  Drosophila melanogaster (lambda J2 clone, pBSr-1Eco subclone) UMP
            synthase (r-l) gene, complete cds.
ACCESSION   L00968
KEYWORDS    UMP synthase.
SOURCE      Drosophila melanogaster (strain Canton S) (library: Maniatis Charon
            4A) embryonic DNA.
  ORGANISM  Drosophila melanogaster
            Eukaryota; Animalia; Metazoa; Arthropoda; Uniramia; Insecta;
            Pterygota; Neoptera; Holometabola; Diptera; Brachycera;
            Cyclorrhapha; Schizophora; Drosophiloidea; Drosophilidae.
REFERENCE   1  (bases 1 to 3710)
  AUTHORS   Eisenberg,M.T., Gathy,K., Vincent,T. and Rawls,J.
  TITLE     Molecular cloning of the UMP synthase gene rudimentary-like in
            Drosophila melanogaster
  JOURNAL   Mol. Gen. Genet. 222, 1-8 (1990)
  STANDARD  full staff_review
REFERENCE   2  (bases 1 to 3710)
  AUTHORS   Eisenberg,M.T., Kirkpatrick,R. and Rawls,J.
  TITLE     Structure of the rudimentary-like gene and UMP synthase in
            Drosophila melanogaster
  JOURNAL   Unpublished (1992)
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     CDS             join(728..865,926..1442,2100..2926)
                     /EC_number="2.4.2.10"
                     /note="Bienzyme protein containing catalytic domains for
                     orotatephosphoribosyltransferase (EC 2.4.2.10) and
                     orotidylatedecarboxylase (EC 4.1.1.23)."
                     /product="UMP synthase; r-l gene product"
                     /gene="r-l"
                     /note="putative"
                     /codon_start=1
                     /translation="MVAQNSDKMRALALKLFEINAFKFGDFKMKVGINSPVYFDLRVI
                     VSLGLPQQTVSDLLVEHIKDKQLSAKHVCGVPYTALPRATIVSVQQGTPMLVRRKEAK
                     AYGTKKLVEGIFNAGDTCLIVEDVVTSGSSILDTVRDLQGEGIVVTDAVVVVDREQGG
                     VANIAKHGVRMHSLFTLSFLLNTLHEAGRIEKSTVEAVAKYIAAVQINSDGTFVGGDK
                     VTFPAANDLQRTKLTYESRANLAKSAVAKRLFNLIASKQTNLCLAADLTHADEILDVA
                     DKCGPYICLLKTHVDIVEDFSDKFIADLQALAQRHNFLLMEDRKFADIGNTVSLQYGK
                     GIYKISSWADLVTAHTLPGRSILQGLKAGLGEGGAGKERGVFLLAEMSASGNLIDAKY
                     KENSNKIATEGADVDFVAGVVCQSSDAFAFPGLLQLTPGVKIDEGVDQLGQQYQSPEH
                     VVKERGADIGVVGRGILKASSPKQAAQTYRDRLWAAYQDRVAK"
                     /citation=[2]
                     /citation=[1]
     exon            615..865
                     /number=1
                     /note="Determined by primer extension analysis of
                     embryonic RNA andsequencing of embryonic cDNA clones."
                     /gene="r-l"
                     /evidence=EXPERIMENTAL
                     /citation=[2]
                     /citation=[1]
     intron          866..925
                     /number=1
                     /note="Determined by sequencing embryonic cDNA clones."
                     /gene="r-l"
                     /evidence=EXPERIMENTAL
                     /citation=[2]
     exon            926..1442
                     /number=2
                     /note="Determined by sequencing embryonic cDNA clones."
                     /gene="r-l"
                     /evidence=EXPERIMENTAL
                     /citation=[2]
     intron          1443..2099
                     /number=2
                     /note="Determined by sequencing embryonic cDNA clones."
                     /gene="r-l"
                     /evidence=EXPERIMENTAL
                     /citation=[2]
     exon            2100..3365
                     /number=3
                     /note="Determined by sequencing embryonic cDNA clones."
                     /gene="r-l"
                     /evidence=EXPERIMENTAL
                     /citation=[2]
                     /citation=[1]
     polyA_signal    3332..3337
                     /gene="r-l"
     polyA_signal    3355..3360
                     /gene="r-l"
     polyA_site      3365..3367
                     /gene="r-l"
                     /evidence=EXPERIMENTAL
                     /citation=[2]
                     /citation=[1]
BASE COUNT     1008 a    875 c    914 g    913 t
ORIGIN
        1 aatagctagc acgttcaatt gcgacttatt tggagaccag cggaagaact aatttggaac
       61 acgcgccaaa ggatctggaa ttgtttcaaa gaatggcgca aacaatgttg acttacgcag
      121 gcgctttggc tttcattttg caatttgagc ttacaggatt gccattggtg gttgcaaaat
      181 gactagactc ctccccttga agttgcagtc aagggtttgt gggcggagcc aaagctttca
      241 gattacaaat gctaagtagt gaacaataca gcaatgcagc ggacaaaata ttgtatataa
      301 tccatccatt catttccttg aaatttctta agacttctca gaaactaaag gttatatatt
      361 ttatgaattt gggaaatatt tcacatgcaa aatagtttat ttcaaaacct taacacttat
      421 gctactttgt taccctataa ctaataataa acgtttatta attcgaagcc aatgttgata
      481 aaatctacga catttataat aatttgaact tcgataagtt tggcggcata gcgaagttaa
      541 ccacccaggc ggcgcaacca gaacttgtaa ccttaattaa ggtgtacgaa atgtgcacca
      601 atgtcacagc agatacgtgc acactggcag catgtttaaa aagcgtagac tcccaattgg
      661 acggttctta ttggagctta gctagcacgt gtgtgaagaa ttaagatcga catttagaat
      721 tgccagcatg gttgcccaga actccgacaa aatgcgggcc ctggccctga agctcttcga
      781 gatcaatgcc ttcaagttcg gtgacttcaa gatgaaagtg ggcataaatt cgccggtcta
      841 cttcgacctc cgagtgatcg tcagttatcc ggatgtgatg gtcagtattt gggattagtg
      901 tctccatgtc aatggcagta gctaacttgg tcttccgcag caaaccgtat ccgacctgct
      961 ggtggaacac atcaaggaca agcagctgag cgccaaacac gtgtgcggtg ttccctacac
     1021 ggcgctcccg cgggccacca ttgtgtccgt ccagcaggga actcccatgc tggtgcggcg
     1081 caaggaggcc aaagcgtacg gcaccaagaa gctggtcgag ggcatcttca atgccggcga
     1141 cacctgtctg attgtcgagg acgtggtcac ctccggctcc agcatcctgg acacggtgcg
     1201 ggatttgcaa ggcgagggca ttgtggtcac cgacgccgtg gtcgtcgtag accgtgagca
     1261 gggcggagtg gccaacatcg ccaagcacgg cgtgcggatg cactcgctct tcacgctttc
     1321 ctttctgctg aacacacttc acgaggccgg gcgcatcgag aagtccacag tggaggcggt
     1381 ggccaagtac atcgccgccg tgcaaatcaa cagcgatggt accttcgtcg gcggcgataa
     1441 gggcgaagtt gtcagaagtg cgtgtgctcc agatccccgt gatatctgcc agatagatag
     1501 atgcatagat agataataag tacatgacac tcggccagca tggcctcgaa caaattcaat
     1561 taggggacgc tccagtcgcg ctactataaa gttatacaac cgctataaac ggcggtaggc
     1621 cgatctggat tccactatcg gaccggatca ttcccgggaa tgcggcacgt gattctgatt
     1681 cagggcagtg tccggtccgc actcaattat tgccgccacg cttatcagcc cactcgagac
     1741 ccaccctgct cctccaatca agcccatctt ctataaataa atcacatttc gtttatggcg
     1801 ctttgataga tgtatgggaa ccggtttgct ttttgtttcc acctgtaaag tcacagtatt
     1861 ctaatctcat cttaatcatc gtgtttgcct ttggggagta acttttgtat atactcttaa
     1921 gatactgtat aaacaatagc caaattgatt ccagttgaaa gagttatgta ctttgggatc
     1981 gccaaagtgt gaagctattg aagaaaattg gcataggtac aaaacaacca tatagcgata
     2041 agaggcgata agataggtct acgtgcacgt ttcaaaattg aagtgttaac taatcaccat
     2101 cactttcccc gcagccaacg acttgcagcg gactaaactg acctacgaga gtcgcgccaa
     2161 tctggctaaa agcgcggtgg ccaagcgcct cttcaacctg atagccagca aacagacgaa
     2221 cctctgcttg gccgccgacc tgacccacgc cgatgagatc ctggacgtgg ccgacaagtg
     2281 cggtccgtac atttgcctgc tgaagacgca cgtagacatt gtggaggatt tcagtgataa
     2341 attcatcgct gacctgcaag ctctggccca gcggcacaat ttcctgctga tggaggatcg
     2401 caagttcgca gatatcggca acacggtgtc cctgcagtac ggcaagggca tctataagat
     2461 ttccagttgg gcagatctgg tcacggccca caccttacct ggacgtagta ttctgcaggg
     2521 tctgaaggcg ggccttggcg aaggaggagc cggcaaggaa cgcggcgtct tcctgctggc
     2581 ggagatgtct gccagcggca acctgatcga cgccaagtac aaggagaaca gcaacaagat
     2641 cgcaaccgag ggagccgatg tagatttcgt tgccggtgtg gtatgccaat cctccgatgc
     2701 ctttgccttc cccggacttc tgcaactgac gcccggtgtg aagatagacg agggcgtgga
     2761 ccaattgggc cagcagtacc agagtccgga gcatgtggtc aaggagcggg gtgccgacat
     2821 cggagtggtg ggcaggggca ttctgaaggc cagcagcccg aagcaggcgg ctcagacgta
     2881 ccgcgaccgc ctgtgggcag cctaccagga tcgagttgcc aagtagaaaa cccaaacagc
     2941 attcccctta acgaatattc cccaataccc attccccctt ggtttcggtt accctagata
     3001 agtgaaaaaa tcttccaatt atttaccgcg attttacagt gcatttatga attgaacata
     3061 ggctatgaat cttccgcaaa acagttggca aacgatgctt gccttaaagt aagagttaca
     3121 acaaatagaa tggttaacaa ctgatccaac gaggaatcct tagtaggagg ccaggcactg
     3181 caagacgtag ctgtagtctc cggagaggtc gtcctggaaa ggagatcagt ggatggtgaa
     3241 tgtgacccag ctttgccgtt gagtgtaagc caactttatt agttaataca agcacaaact
     3301 acgcatacga ctttgatgcg ccataattta caataaaaaa agacatgtat acaaaataaa
     3361 aatacgtaaa aatgttaaat aatcgttgaa cgttagacgt tattgcacag agggaaaata
     3421 ctaagcgttt gtagattgga tttttagaac acatcacaac tactgtatat agaccaactc
     3481 catttgttct tcgcttttgt tatacttttt ttccggggaa atgcaacgtt tttttttttg
     3541 gcattttcta cttgaacagt ggggtattgc tcatagatat tcggatattc cggctgcttc
     3601 cgtctaccaa gccgtaagag tgaccaggac gatccaatat cggtctcggc atcctcctgt
     3661 tgttggttcg ttaaacggta gagaaagcgg aaagagcaga ggtcagatgc
//