From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Streptomyces coelicolor (clones phiC31 and KC304) dnaK gene,
Date: 3 Jan 93 22:26:03 GMT
Distribution: bionet
Checksum: 03850 6

LOCUS       STMDNAK      2550 bp ds-DNA             BCT       24-DEC-1992
DEFINITION  Streptomyces coelicolor (clones phiC31 and KC304) dnaK gene,
            complete cds; grpE gene, 5' end cds.
ACCESSION   L08201
KEYWORDS    dnaK gene; grpE gene.
SOURCE      Streptomyces coelicolor (strain A3(2)) (library: lambda-EMBL3) DNA.
  ORGANISM  Streptomyces coelicolor
            Prokaryotae; Firmicutes; Streptomycetaceae.
REFERENCE   1  (bases 1 to 2550)
  AUTHORS   Bucca,G., Smith,C.P., Alberti,M., Seidita,G., Passantino,R. and
            Puglia,A.M.
  TITLE     Cloning and sequencing of the dnaK region of Streptomyces
            coelicolor A3(2)
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     RBS             186..191
                     /gene="dnaK"
     RBS             2039..2043
                     /gene="grpE"
     CDS             200..2056
                     /gene="dnaK"
                     /codon_start=1
                     /translation="MARAVGIDLGTTNSVVSVLEGGEPTVITNAEGARTTPSVVAFAK
                     NGEVLVGEVAKRQAVTNVDRTIRSVKRHMGTDWKVNLDGKDFNPQQISAFVLQKLKRD
                     AESYLGEKVTDAVITVPAYFNDAERQATKEAGEIAGLNVLRIVNEPTAAALAYGLDKD
                     EQVILVFDLGGGTFDVSLLEIGDGVVEVKATNGDNNLGGDDWDQRVVDYLVKQFQSGH
                     GVDLAKDKMALQRLREAAEKAKIELSSSTETTINLPYITASAEGPLHLDEKLTRAQFQ
                     QLTSDLLERCKTPFHNVIKDAGIQLSEIDHVVLVGGSTRMPAVAELVKELTGGKDANK
                     GVNPDEVVAIGAALQAGVLKGEVKDVLLLDVTPLSLGIETKGGIMTKLIERNTTIPTK
                     RSEIFTTAEDNQPSVQIQVYQGEREIAAYNKKLGMFELTGLPPAPRGVPQIEVAFDID
                     ANGIMHVTAKDLGTGKEQKMTVTGGSSLPKDEVDRMRQEAEKYAEEDHARREAAESRN
                     QGEQLVYQTEKFLKDNEDKVPGEVKTEVESAVAELKEKLKGEDTAEIRTATEKVAAVS
                     QKLGQAMYADAQAAQAAGGEAPGADAGAEGKGADDDVVDAEIVDDERKDGAA"
     CDS             2053..>2550
                     /gene="grpE"
                     /codon_start=1
                     /translation="MTEETPGFEEKPDVPSGATPDDAEPQAASEEGAAPAGDASENAG
                     LVAQLDQVRTALNERTADLQRLQAEYQNYRRRVERDRVAVKEVAVANLLSELLPVLDD
                     VGRAREHGELVGGFKSVAESLETTVAKLGLQQFGKEGEPFDPTIHEALMHSYAPDVTE
                     TTCVAI"
BASE COUNT      484 a    883 c    860 g    323 t
ORIGIN      
        1 ccgcgggcgg tgcgtccttc cgggcggcga tttttccggt tcgtcaggcc acgagtaccg
       61 agaacgtgca ggtcggggcg ccgttgcgtg taaggaacgg ataagagttg agtccgctcg
      121 actcacctct gttgacccat cgccgggcgt catgcacact tgagcctgtt ccactcaagt
      181 cagctggagg ttattcacca tggcacgtgc ggtcggcatc gacctgggca cgactaactc
      241 cgtcgtcagc gttctggagg gcggcgagcc caccgtcatc accaacgccg agggcgccag
      301 gaccacgccg tccgtcgtcg ccttcgccaa gaacggcgag gtgctcgtcg gcgaggtggc
      361 caagcgccag gcggtcacga acgtcgacag gaccatccgc tcggtcaagc gccacatggg
      421 caccgactgg aaggtcaacc tggacggcaa ggacttcaac ccgcagcaga tcagcgcctt
      481 cgtgctgcag aagctgaagc gggacgccga gtcctacctg ggcgagaagg tgaccgacgc
      541 ggtcatcacc gtcccggcgt acttcaacga cgccgagcgc caggccacca aggaggccgg
      601 tgagatcgcg ggcctcaacg tcctgcgcat cgtcaacgag ccgaccgccg ccgcgctggc
      661 gtacggcctc gacaaggacg agcaggtcat cctggtcttc gacctcggtg gcggtacgtt
      721 cgacgtctcg ctgctggaga tcggcgacgg cgtcgtcgag gtgaaggcca ccaacggtga
      781 caacaacctc ggtggtgacg actgggacca gcgcgtcgtc gactacctgg tcaagcagtt
      841 ccagtccggc cacggcgtgg acctggccaa ggacaagatg gcgctccagc gcctgcgcga
      901 ggcggccgag aaggccaaga tcgagctgtc ctcgtccacc gagaccacga tcaacctccc
      961 ctacatcacg gcctccgccg agggcccgct gcacctggac gagaagctca cccgcgccca
     1021 gttccagcag ctgacctccg acctgctgga gcgctgcaag acgccgttcc acaacgtcat
     1081 caaggacgcc ggcatccagc tgtccgagat cgaccacgtc gtcctcgtcg gcggctccac
     1141 ccgtatgccc gccgtcgccg agctggtcaa ggagctgacc ggcggcaagg acgccaacaa
     1201 gggcgtcaac ccggacgagg tcgtcgccat cggcgccgcc ctgcaggccg gtgtcctcaa
     1261 gggcgaggtc aaggacgtcc tgctgctcga cgtcaccccg ctgtccctcg gcatcgagac
     1321 caagggcggc atcatgacca agctgatcga gcggaacacg acgatcccga ccaagcgctc
     1381 ggagatcttc accaccgccg aggacaacca gccctccgtg cagatccagg tctaccaggg
     1441 cgagcgcgag atcgcggcgt acaacaagaa gctcgggatg ttcgagctga ccggtctgcc
     1501 gccggcgccc cgcggcgtcc cgcagatcga ggtcgccttc gacatcgacg ccaacggcat
     1561 catgcacgtg accgcgaagg acctgggcac gggcaaggag cagaagatga ccgtcaccgg
     1621 cggctcctcg ctgccgaagg acgaggtcga ccggatgcgc caggaggccg agaagtacgc
     1681 ggaggaggac cacgcccgcc gcgaggccgc cgagtcccgc aaccagggcg agcagctcgt
     1741 ctaccagacg gagaagttcc tcaaggacaa cgaggacaag gtccccggtg aggtcaagac
     1801 cgaggtggag tccgccgtcg ccgagctgaa ggagaagctc aagggcgagg acaccgccga
     1861 gatccgcacc gccacggaga aggtcgccgc cgtctcgcag aagctgggcc aggccatgta
     1921 cgccgacgcc caggccgcgc aggccgccgg cggcgaggcc ccgggcgccg acgccggtgc
     1981 cgagggcaag ggtgccgacg acgacgtcgt cgacgccgag atcgtggacg acgagcgcaa
     2041 ggacggtgcc gcgtgacgga ggagaccccg ggtttcgagg agaagcccga cgtcccctcc
     2101 ggcgccaccc ctgacgacgc cgagccgcag gccgcctccg aagagggggc ggccccggcc
     2161 ggggacgcga gcgagaacgc gggcctggtg gcccagctgg accaggtgcg caccgcgctg
     2221 aacgagcgca ccgcggacct ccagcggctc caggccgagt accagaacta ccgccgccgg
     2281 gtggagcggg accgcgtcgc ggtcaaggag gtcgccgtcg cgaacctcct gtccgagctg
     2341 cttcccgtgc tcgacgacgt cgggcgcgcc cgggagcacg gcgaactggt cggcggcttc
     2401 aagtccgtcg cggagtcgct ggagaccacg gtcgccaagc tgggcctcca gcagttcggc
     2461 aaggagggcg agcccttcga cccgacgatc cacgaggccc tgatgcactc ctacgcaccg
     2521 gacgtcaccg agacgacctg cgtggcgatc
//