From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Cow prostaglandin F synthetase II (PGFSII) mRNA, complete cds.
Date: 21 Aug 92 10:05:48 GMT
Distribution: bionet
Checksum: 13303     4

LOCUS       BOVPRGFSII   1229 bp ss-mRNA            MAM       21-AUG-1992
DEFINITION  Cow prostaglandin F synthetase II (PGFSII) mRNA, complete cds.
ACCESSION   M86544
KEYWORDS    aldo-keto reductase; prostaglandin D-2;
            prostaglandin F synthetase II; prostaglandin H-2.
SOURCE      Bos taurus liver cDNA to mRNA.
  ORGANISM  Bos taurus
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Artiodactyla; Ruminantia; Pecora; Bovidae; Bovinae;
            Bovini.
REFERENCE   1  (bases 1 to 1229)
  AUTHORS   Kuchinke,W., Barski,O., Watanabe,K. and Hayaishi,O.
  TITLE     A lung type prostaglandin F synthase is expressed in bovine liver:
            cDNA sequence and expression in E. coli
  JOURNAL   Biochem. Biophys. Res. Commun. 183, 1238-1246 (1992)
  STANDARD  full staff_entry
FEATURES             Location/Qualifiers
     CDS             16..987
                     /EC_number="1.1.1.2"
                     /product="prostaglandin F synthetase II"
                     /gene="PGFSII"
                     /evidence=EXPERIMENTAL
                     /codon_start=1
                     /translation="MDPKSQRVKFNDGHFIPVLGFGTYAPEEVPKSEALEATKFAIEV
                     GFRHVDSAHLYQNEEQVGQAIRSKIADGTVKREDIFYTSKLWCNSLQPELVRPALEKS
                     LQNLQLDYVDLYIIHSPVSLKPGNKFVPKDESGKLIFDSVDLCHTWEALEKCKDAGLT
                     KSIGVSNFNHKQLEKILNKPGLKYKPVCNQVECHPYLNQSKLLEFCKSHDIVLVAYAA
                     LGAQLLSEWVNSNNPVLLEDPVLCAIAKKHKQTPALVALRYQVQRGVVVLAKSFNKKR
                     IKENMQVFDFELTPEDMKAIDGLNRNTRYYDFQQGIGHPEYPFSEEY"
     mutation        45
                     /note="the conserved leu is replaced by phe"
                     /gene="PGFSII"
BASE COUNT      365 a    256 c    290 g    318 t
ORIGIN
        1 gaggagcaac aaacaatgga tcccaaaagc cagagggtga agtttaatga tggccacttc
       61 attcctgtcc tgggatttgg aacctatgca cctgaggagg ttcctaagag tgaagccctg
      121 gaggccacca aatttgctat agaggttggg ttccgccatg tggacagtgc tcatttgtat
      181 caaaatgagg agcaggttgg ccaggccatt cgaagcaaga ttgcagatgg cactgtgaag
      241 agagaagaca tattctacac ttcaaagctt tggtgcaatt cccttcaacc agagttggtc
      301 cgaccagcct tggaaaagtc attgcaaaat cttcaactgg actatgtcga tctctatatt
      361 attcattctc cagtgtctct gaagccaggg aataaatttg ttccaaaaga tgaaagtgga
      421 aaactgatat ttgactcggt ggatctctgt cacacgtggg aggccctgga gaagtgtaag
      481 gacgcagggc tgaccaagtc cattggggtg tccaacttca accacaagca gctagagaag
      541 atcctgaaca agccggggct caagtacaag cccgtctgca accaggtgga atgtcaccct
      601 tacctcaacc agagcaaact gttagagttc tgcaagtcac atgatattgt cctagttgct
      661 tatgctgctc tgggagccca actattgtca gaatgggtga actcaaacaa ccccgttctc
      721 ttggaggacc cggttctttg tgccattgcc aaaaagcaca agcagacccc agctctggtt
      781 gcccttcgct accaggtaca acgtggagtt gtggttctgg ccaagagttt caacaagaag
      841 aggatcaaag agaatatgca ggtgtttgac tttgaactga ctccggaaga tatgaaagca
      901 atcgatggcc tcaatcgtaa tacaagatac tatgattttc aacagggtat tggtcaccct
      961 gagtacccat tttctgaaga atattaactg ggtgagctgt ccaccatggc ttctacctga
     1021 acgtctgctt ctagggctac gaagagcgtg tctatacttg gtggaggtgt ttaaaagaag
     1081 tgcctgaact tttgaaagat tgtttttctt taaaaattct ttatgaaata accaagattt
     1141 caaatatggg tactagtttt tcctaacaaa ataatttgaa aaataaaagg gaaaagatag
     1201 aaaataaaga taacctggtt aacccattt
//