From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Rat ketohexokinase mRNA, complete cds.
Date: 29 Sep 92 10:08:33 GMT
Distribution: bionet
Checksum: 65200     4

LOCUS       RATKETOHEX   1342 bp ss-mRNA            ROD       29-SEP-1992
DEFINITION  Rat ketohexokinase mRNA, complete cds.
ACCESSION   M86235 X63658
KEYWORDS    furanose kinase; ketohexokinase; phosphotransferase.
SOURCE      Rattus norvegicus (strain Sprague-Dawley) (library: lambda ZAP)
            adult liver cDNA to mRNA.
  ORGANISM  Rattus norvegicus
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Rodentia; Myomorpha; Muridae; Murinae.
REFERENCE   1  (bases 1 to 1342)
  AUTHORS   Donaldson,I.A., Doyle,T.C. and Matas,N.
  TITLE     Expression of rat liver ketohexokinase in yeast results in fructose
            intolerance
  JOURNAL   Unpublished (1992)
  STANDARD  full staff_entry
FEATURES             Location/Qualifiers
     5'UTR           1..259
     misc_signal     62..64
                     /function="in-frame upstream stop codon"
     CDS             260..1156
                     /function="ATP: beta-D-fructose 1-phosphotransferase,
                     primary metabolism of dietary fructose in mammals"
                     /EC_number="2.7.1.3"
                     /product="ketohexokinase"
                     /evidence=EXPERIMENTAL
                     /codon_start=1
                     /translation="MEEKQILCVGLVVLDIINVVDKYPEEDTDRRCLSQRWQRGGNAS
                     NSCTVLSLLGARCAFMGSLAHGHVADFLVADFRRRGVDVSQVAWQSQGDTPCSCCIVN
                     NSNGSRTIILYDTNLPDVSAKDFEKVDLTRFKWIHIEGRNASEQVKMLQRIEQYNATQ
                     PLQQKVRVSVEIEKPREELFQLFGYGEVVFVSKDVAKHLGFRSAGEALKGLYSRVKKG
                     ATLICAWAEEGADALGPDGQLLHSDAFPPPRVVDTLGAGDTFNASVIFSLSKGNSMQE
                     ALRFGCQVAGKKCGLQGFDGIV"
     3'UTR           1157..1342
     polyA_signal    1323..1328
     polyA_site      1342
                     /evidence=EXPERIMENTAL
BASE COUNT      280 a    375 c    409 g    278 t
ORIGIN
        1 gcagccagac gccaggctgg atgaaatgaa gaaggcaatg gcaggcagtg gtgccagcct
       61 ctgaggtgtc gagctgctac ctcccaccgc tcatctctca cacaatgcca tcaaacccct
      121 gcaagccaca cttcttgcca gccacctggc acccgaatct cagggcctcc tgcatgctgt
      181 ttcccttgga gaggctgaag agacagagtc tagcaggaat cccctccgct tgcgggtagg
      241 aagcttgggg agcagcctca tggaagagaa gcagatcctg tgcgtggggc tggtggtgct
      301 ggacatcatc aatgtggtgg acaaataccc agaggaagac acggatcgca ggtgcctatc
      361 ccagagatgg cagcgtggag gcaacgcgtc caactcctgc actgtgcttt ccttgctcgg
      421 agcccgctgt gccttcatgg gctcgctggc ccatggccat gttgccgact tcctggtggc
      481 cgacttcagg cggaggggtg tggatgtgtc tcaagtggcc tggcagagcc agggagatac
      541 cccttgctcc tgctgcatcg tcaacaactc caatggctcc cgtaccatta ttctctacga
      601 cacgaacctg ccagatgtgt ctgctaagga ctttgagaag gtcgatctga cccggttcaa
      661 gtggatccac attgagggcc ggaatgcatc ggaacaggta aagatgctac agcggataga
      721 acagtacaat gccacgcagc ctctgcagca gaaggtccgg gtgtccgtgg agatagagaa
      781 gccccgagag gaactcttcc agctgttcgg ctatggagag gtggtgtttg tcagcaaaga
      841 tgtggccaag cacctggggt tccggtcagc aggggaggcc ctgaagggct tgtacagtcg
      901 tgtgaagaaa ggggctacgc tcatctgtgc ctgggctgag gagggagccg atgccctggg
      961 ccccgacggc cagctgctcc actcagatgc cttcccacca ccccgagtag tagacactct
     1021 cggggctgga gacaccttca atgcctctgt catcttcagc ctctccaagg gaaacagcat
     1081 gcaggaggcc ctgagattcg ggtgccaggt ggctggcaag aagtgtggct tgcaggggtt
     1141 tgatggcatt gtgtgagaga tgagcggtgg gaggtagcag ctcgacacct cagaggctgg
     1201 caccactgcc tgccattgcc ttcttcattt catccagcct ggcgtctggc tgcccagttc
     1261 cctgggccag tgtaggctgt ggaacgggtc tttctgtctc ttctctgcag acacctggag
     1321 caaataaatc ttcccctgag cc
//