From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: D.pseudoobscura Adh gene for alcohol dehydrogenase
Date: 28 Dec 92 21:26:55 GMT
Distribution: bionet
Checksum: 00755 8

LOCUS       DROADHGN29   3457 bp ds-DNA             INV       01-DEC-1992
DEFINITION  D.pseudoobscura Adh gene for alcohol dehydrogenase
ACCESSION   X62209
KEYWORDS    ADH gene; alcohol dehydrogenase.
SOURCE      Drosophila pseudoobscura DNA.
  ORGANISM  Drosophila pseudoobscura
            Eukaryota; Animalia; Metazoa; Arthropoda; Uniramia; Insecta;
            Pterygota; Neoptera; Holometabola; Diptera; Brachycera;
            Cyclorrhapha; Schizophora; Drosophiloidea; Drosophilidae.
REFERENCE   1  (bases 1 to 3457)
  AUTHORS   Schaeffer,S.W. and Miller,E.L.
  TITLE     Estimates of gene flow in Drosophila pseudoobscura determined from
            nucleotide sequence analysis of the alcohol dehydrogenase region
  JOURNAL   Genetics 132, 471-480 (1992)
  STANDARD  full automatic
COMMENT     *source: strain=GB66; *source: isolate=Gundlach Bundschu Winery,
            Sonoma Valley, *source: California; *source: chromosome=IV section
            88;
               From EMBL    entry DPADHGN29;  dated 16-OCT-1992.
FEATURES             Location/Qualifiers
     mRNA            join(40..119,916..1021,1085..1489,1550..1965)
                     /note="adult mRNA (Adh)"
     mRNA            join(883..1021,1085..1489,1550..1965)
                     /note="larval mRNA (Adh)"
     promoter        8..14
                     /note="adult promoter (Adh)"
     prim_transcript 40..1965
                     /note="adult primary transcript (Adh)"
     exon            40..119
                     /note="adult exon 1a"
     intron          120..915
                     /note="adult intron"
     promoter        847..854
                     /note="larval promoter (Adh)"
     prim_transcript 883..1965
                     /note="larval primary transcript (Adh)"
     exon            883..1021
                     /note="larval exon 1"
     exon            916..1021
                     /note="adult exon 1b"
     intron          1022..1084
                     /number=1
     exon            1085..1489
                     /number=2
     intron          1490..1549
                     /number=2
     exon            1550..1965
                     /number=3
     polyA_signal    1942..1947
     polyA_site      1965
     CDS             join(929..1021,1085..1489,1550..1816)
                     /EC_number="1.1.1.1"
                     /gene="Adh"
                     /product="alcoholdehydrogenase"
                     /codon_start=1
                     /translation="MSLTNKNVVFVAGLGGIGLDTSRELVKRNLKNLVILDRIDNPAA
                     IAELKAINPKVTITFYPYDVTVPVAETTKLLKTIFAQVKTIDVLINGAGILDDHQIER
                     TIAVNYTGLVNTTTAILDFWDKRKGGPGGIICNIGSVTGFNAIYQVPVYSGSKAAVVN
                     FTSSLAKLAPITGVTAYTVNPGITKTTLVHKFNSWLDVEPRVAEKLLEHPTQTSQQCA
                     ENFVKAIELNKNGAIWKLDLGTLEPITWTQHWDSGI"
BASE COUNT      966 a    790 c    798 g    903 t
ORIGIN      
        1 cgtagtgtat ttaaggcact gcacatgtcg aggaccacga ttattgtcta cagtgcagtc
       61 agtagttgtc aggtgcagca ggcggaccag catttcttta aatctactca atcgatcaag
      121 taagtaactg taatccgagt acagtcgacc catcccactc ggttctgccg tagctcaaga
      181 aatttcgtga tagcgcagtt ccagttgtaa aacgtcgttt aaaaaaacat ctcgttggag
      241 cttgggttga aaaacgttcg agttgggcgt agaacatttc aaatataaaa taacgcaaaa
      301 gagaaaatat cttcccaaaa gcagctgtcc attttttgtt aaagtgatag atcaaaaatt
      361 cgtatgacgt atttcccaag tgctctttat gcaaataatt tcctcaattc taattctaca
      421 agaagcactt ttggctcttg tccagaaaaa acaaattgtt tacacttttc ggttgataac
      481 agaagcggac cgacggctag gtaacataac agtcgcctgt taagtaatgc cctcactctc
      541 tgttagatgg ccgcgcgtgt cgactccgat agaccagcag acacgcagta gttttcctca
      601 agaacctgaa ctctaaacat agacataatt tacgatacct tgagactcaa ataaaaatac
      661 aagtatgagt ggtattctat tcctgattag actcgcacac acacacgtac gagtattaac
      721 ataaatttgc cggcaaataa ccaaaacaaa acgaacgcaa acgatccgaa cacgtaatgc
      781 gagagataag aaacagaaaa gctcgacgtg aaagaagctt ctggcggaag ctcttgccga
      841 actccctata aatacgagac agaaaaccag aataaatata ataacaaact gttgaattgt
      901 cccatcgatt cccagatcaa aagacagaat gtctctcacc aacaagaacg tcgttttcgt
      961 ggccggtctg ggcggcattg gcctggacac cagccgggag ttggtcaagc gtaatctgaa
     1021 ggtaagagag aacgaattcc atggaatcta tggaatccga aatttaaaaa ttaatttatt
     1081 ttagaacctg gtcatcctgg atcgcattga caatccggct gccattgccg agctgaaggc
     1141 aatcaatccc aaggtgacca tcaccttcta tccctacgat gtgactgtgc ccgtcgctga
     1201 gaccaccaag ctcctgaaga ccatctttgc ccaggtgaag acaatcgatg tcctgatcaa
     1261 cggtgctggc atcctggacg atcatcagat cgagcgcacc attgccgtta actacacggg
     1321 cctggtcaac accaccacag ccattctgga cttctgggac aagcgcaagg gcggcccagg
     1381 aggcatcatt tgcaacattg gctccgtcac cggtttcaat gccatctacc aggtgcccgt
     1441 ttactccggc tccaaggcgg cggtggttaa ctttacctcc tccctggcgg taagtaatcc
     1501 aacgtttttc ctactcccca aaactaaata tttctttgaa attccataga aactggctcc
     1561 cattactggt gtcactgctt acactgtcaa tcctggcatc actaagacca ctctggtcca
     1621 caaattcaac tcgtggctgg atgtggagcc ccgtgtggca gagaagctgc tcgagcatcc
     1681 cacccagacc tctcagcagt gcgccgagaa ctttgtgaag gccatcgagc tgaacaagaa
     1741 cggtgccatc tggaagttgg acttgggcac cttggagccc atcacatgga cccagcactg
     1801 ggactcgggc atctaaacca gcccagagac tctatgggac atggcgtttt agcttttagt
     1861 ttcttttttt ccactcaatt gttacgtata tacatacaca tatggcaata aggctgattt
     1921 gaaccccgtt ttgaatatga taatacaaaa ttatatttga gaaatttcaa caaaatcgat
     1981 aagagctata gctctctttt aattaaaaaa actagataaa aagagcaatg gtcagtggtg
     2041 gtcagtggtc gttgtggcct ctatcgattt ccacacaaaa actttactgt ttagtaatag
     2101 aaaagaactt gagaaaggca gccaaaagaa tgtacgatct gacgggtaag catgtctgct
     2161 atgtggctga ctgcggtggc attgcactgg agaccagcaa ggttctcatg accaagaata
     2221 tagcggtgag tggtggagag gtggtgtaga gagtggagag tggttctggt tcttaaacaa
     2281 gtctctagtc tcttggctgc tgctggtagc tgtcgaaact aattaagtta atgacttttt
     2341 catgttttgt ttttgtccgc tgacttttgc tgttcaaagc tacaattaag tttatgtttt
     2401 gagtttgatc tacttttgct taagggaagt actttcgatt acggattgct ggcatataaa
     2461 aatagatacc aaggaatgtg catcatattg attctatctt gattctttgc ctagaaactg
     2521 gccatccttc agagcgtgga aaaccccccg gccatcgccc agttacaatc gattaagcac
     2581 agcacacaga tcttcttctg gaccttcgat gtgaccatgg cccgcgagga gatgaagaag
     2641 tacttcgatg aggtcatggt ccagatggac tacatcgatg tgctgatcaa tggggccacc
     2701 ctgtgcgatg agcggaacat tgatgccacc atcaatacga atctgaccgg catgatgaac
     2761 accgtggcca ctgtgctgcc ctatatggac cgtaagatgg gcggttcggg tggactgatt
     2821 gtgaatgtca cctccgtcat aggactggat ccatcgccgg tcttttgtgc gtacagtgcc
     2881 tcaaagtttg gtgtgattgg gttcacccga agtctagcgg tgagtgggcg agatcttaac
     2941 ttgaaaatcc tctctaatgt cttccattct tccgcaggat cccctgtatt acacccaaaa
     3001 tggtgtggct gtcatggccg tctgctgtgg ccccaccaaa gtgtttgtcg atcgcgaact
     3061 gactgccttc ctggaatacg gccaaacctt tgccgatcgc ctgcgccgtg ccccctgcca
     3121 atcgacggcc gtctgcggca agaatatagt taatgccatc gagagatcgg agaatggaca
     3181 gatctggatt gccgacaagg gcggcctgga gtcggtggcc ctccactggt attggcatat
     3241 ggccgatcag tttgtgaact atatgcagag cactgacgat gaggatcagg agtttttttt
     3301 gggccagcga tgaggcatgc cttgatccac ggaataaaac atcaaatgtg tcacaaaaag
     3361 taggcaactt gtttgtgggt ttggaaatag atttgaaata ttttgaggtc atatgaataa
     3421 tgacataaat gagggtgcct gaaagtatgc caagttt
//