From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Murine cytomegalovirus immediate-early protein 1 (ie1) gene,
Date: 22 Dec 92 22:52:58 GMT
Distribution: bionet
Checksum: 37145 6

LOCUS       HS5IE1IE3A   1802 bp ds-DNA             VRL       30-NOV-1992
DEFINITION  Murine cytomegalovirus immediate-early protein 1 (ie1) gene,
            partial cds; immediate-early protein 3 (ie3) gene, exon 5 and
            partial cds.
ACCESSION   M77846
KEYWORDS    alternative splicing; early promoter trans-activator;
            ie1/ie3 gene complex; immediate-early gene;
            immediate-early promoter repressor; immediate-early protein 1;
            immediate-early protein 3; nuclear protein; regulatory protein.
SOURCE      Murine cytomegalovirus (strain Smith) (library: ATCC VR-194) DNA.
  ORGANISM  Murine cytomegalovirus
            Viridae; ds-DNA enveloped viruses; Herpesviridae;
            Betaherpesvirinae.
REFERENCE   1  (sites)
  AUTHORS   Ohama,T., Yamao,F., Muto,A. and Osawa,S.
  TITLE     Organization and codon usage of the streptomycin operon in
            Micrococcus luteus, a bacterium with a high genomic G+C content
  JOURNAL   J. Bacteriol. 169, 4770-4777 (1987)
  STANDARD  full automatic
REFERENCE   2  (bases 1 to 1802)
  AUTHORS   Messerle,M., Buehler,B., Keil,G.M. and Koszinowski,U.H.
  TITLE     Structural organization, expression, and functional
            characterization of the murine cytomegalovirus immediate-early gene
            3
  JOURNAL   J. Virol. 66, 27-36 (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     mRNA            1..43
                     /gene="ie1"
                     /evidence="EXPERIMENTAL"
                     /partial
     conflict        25..32
                     /evidence="EXPERIMENTAL"
                     /citation=[1]
                     /note="conflict with M17788"
     misc_feature    45..70
                     /evidence="EXPERIMENTAL"
                     /note="splice acceptor"
     exon            71..1772
                     /number=5
                     /gene="ie3"
                     /evidence="EXPERIMENTAL"
     mRNA            71..1772
                     /gene="ie3"
                     /evidence="EXPERIMENTAL"
                     /partial
     CDS             71..1608
                     /gene="ie3"
                     /evidence="EXPERIMENTAL"
                     /partial
                     /product="immediate-early IE3 protein"
                     /codon_start=3
                     /translation="PEMNIDIVTAAASMSGITTASHSALPIRRPNTPDFRRHSEKKAK
                     KHKNKQRQRLDDVVAKLHKPSESEADEDFIFQQPQEEEYQEEQEPQVISSSPQHFDER
                     SPSRSSSDHGGHSGGNSRDGQFYLSSGSEDEDDEDDERVDSGYRGSSRSEDSSRYRPH
                     GGSHSSRSSIKSSGSGSSRHHHKRKAVPERHHPFTPPSAKRYAAAAPSSRYECPVRVD
                     SSDSDDAPTMVQRGTLKIKSFRPPSSGSNSNKHSSSSGGSTSSSHKKQQQQQAPSKKP
                     VMSSGSDKIRDMVDRTAGGYVAPNAHKKCREDKSRKYPARALEYKNLPFRPQSPQYLL
                     GKAIQFCKEETVHDKFIMLFYTRSQDVRKAVDETRARMGMRPNLSISCPFMTEHTKPI
                     NHSRETIDRTSAACTAGTQAVWDMEERRGQKCVPRTSDYRSMIIQAANPPDFLGAVKT
                     CLHLSQVFPKQVCMRLCSITGGLNPLPIYEETVSSYVNAQFEADDISHHEDESGEYES
                     DCE"
BASE COUNT      498 a    445 c    472 g    387 t
ORIGIN      
        1 aatgttttct gaataaagtg atcctattgt acccacatta aagacttctt taactcttta
       61 tggttcacag gacccgagat gaacatagat attgttacag cagcggcctc catgtcaggt
      121 ataactactg cctcacacag cgccctgcca atcagaagac caaacacccc agacttcaga
      181 agacactcag aaaagaaagc taagaagcat aagaacaagc agcgccagag acttgatgat
      241 gtagttgcta agttacataa gccctctgag agtgaggcag atgaggactt tatttttcag
      301 cagcctcagg aggaggagta tcaggaggag caggagcccc aggtgatctc ctcctctcct
      361 caacactttg atgagaggag ccccagcagg tcttcctcag atcatggtgg gcacagtggg
      421 ggaaacagta gagatggaca gttctatctg tctagtggtt ctgaggatga agatgatgag
      481 gatgatgagc gggtagatag tggctacagg gggagcagta ggagtgaaga cagcagcagg
      541 tacaggccac acggaggctc ccactcatca aggtcctcca ttaaatcatc agggagcggg
      601 tcctctagac accaccacaa gaggaaggcg gtacctgaac gccaccatcc tttcacacct
      661 ccctctgcga agaggtatgc cgccgctgcc cccagcagca gatatgagtg tcctgtgcgt
      721 gtggattcct cagacagtga tgatgcccct accatggtac agagggggac cctgaagatc
      781 aagtccttca ggccccctag ctctggttcc aactccaaca aacacagcag cagctctggt
      841 gggtctacta gtagtagcca caagaagcaa cagcagcagc aggcccctag caagaagcct
      901 gtgatgagta gtgggagtga taagatcaga gacatggtag acaggacagc cggaggctac
      961 gtggccccca acgcccacaa gaagtgcagg gaagataagt ccaggaagta cccagccagg
     1021 gccttggagt acaagaacct gcccttcaga cctcagtccc cacagtatct actgggtaag
     1081 gccatccagt tctgtaagga ggagaccgtg catgacaagt tcatcatgct gttctacacc
     1141 aggagtcagg atgtgaggaa ggcagtggat gagacacgcg cccgcatggg gatgcgcccc
     1201 aacctgtcca tctcctgccc cttcatgaca gagcacacca agcccattaa ccacagcagg
     1261 gagaccatag atagaacctc agcggcctgc accgcaggta ctcaggcagt gtgggacatg
     1321 gaggagagac gaggtcagaa gtgtgtgccc aggacctcag actacaggag catgataatt
     1381 caggcagcca accctccaga cttcttggga gcggtgaaga cctgtctgca tttgagtcag
     1441 gtcttcccca agcaggtgtg catgaggctc tgcagcataa caggcgggtt gaatcctctt
     1501 cccatctatg aggagactgt cagcagttat gtgaacgccc agtttgaggc agatgatata
     1561 agccatcatg aggatgagtc tggggagtat gagtctgact gcgagtgatc tgtactgtgg
     1621 acttggtatt aaagatgaac cctgttttgt aaaatatgta gatacataac taactaacta
     1681 actgtctgtg tatatatttg attgagacct gagaccccca atcttaactc atgtcgaacc
     1741 ctattaaata aatccttttt gatatatatt gagcacttgt gtcttgtccc tctttgtctc
     1801 tg
//