From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: shufflon region: pilU...rci=site-specific recombinase [IncI2
Date: 26 Jan 93 05:50:41 GMT
Distribution: bionet
Checksum: 46816 13

LOCUS       S117386      5058 bp    DNA             BCT       25-JAN-1993
DEFINITION  shufflon region: pilU...rci=site-specific recombinase [IncI2
            plasmid R721, Plasmid, 4 genes, 5058 nt]
ACCESSION   S47694 X62169
KEYWORDS    .
SOURCE      IncI2 plasmid R721
  ORGANISM  Unclassified.
            Unclassified.
REFERENCE   1  (bases 1 to 5058)
  AUTHORS   Kim,S.R. and Komano,T.
  TITLE     Nucleotide sequence of the R721 shufflon.
  JOURNAL   J. Bacteriol. 174, 7053-7058 (1992)
  STANDARD  full automatic
COMMENT     This entry [NCBI gibbsq 117386] was created by the journal scanning
            component of NCBI/GenBank at the National Library of Medicine.
            This sequence comes from Fig. 2.
            Region: shufflon region.  
FEATURES             Location/Qualifiers
     CDS             29..664
                     /gene="pilU"
     CDS             complement(2996..3337)
                     /partial
                     /note="For the protein sequence (NCBI gibbsq 118198):
                     Method: conceptual translation supplied by author.  This
                     sequence comes from Fig. 4."
                     /gene="pilV"
                     /product="PilV variable region A"
                     /codon_start=1
                     /translation="VWRTSGSSNGSYSNLGSHRGSFTGRNTGSGTLFVYASGGNGGSA
                     GGDCANTSRLQGYVAGALISTNASNNPSYGKTAFISFAVPAGATYQITSYPAQNYSCG
                     SGVFSVFGYQT"
     CDS             2790..2999
                     /partial
                     /note="Description: PilV variable region A;  variable
                     region A';  For the protein sequence (NCBI gibbsq 117388):
                      Method: conceptual translation supplied by author.  This
                     sequence comes from Fig. 4."
                     /gene="pilV"
                     /product="PilV"
                     /codon_start=1
                     /translation="VWRALGGKLKVTQLSSTGYLGQFDFCAIARMGNAEDSHYCQVVE
                     SPSGSRKWYKYEHKTGCIASCVTLN"
     CDS             complement(2504..2755)
                     /partial
                     /note="Description: PilV variable region A;  variable
                     region B';  For the protein sequence (NCBI gibbsq 117389):
                      Method: conceptual translation supplied by author.  This
                     sequence comes from Fig. 4."
                     /gene="pilV"
                     /product="PilV"
                     /codon_start=1
                     /translation="TWKKIGAGDSQIVTASATAWRWPGATATCPSGKKVIGGGGQCRS
                     NTGFIWLTRSMPSGNNAWTASCDTTEDQNGSITVYAICQ"
     CDS             complement(1927..2178)
                     /partial
                     /note="Description: PilV variable region A;  variable
                     region C;  For the protein sequence (NCBI gibbsq 117390):
                     Method: conceptual translation supplied by author.  This
                     sequence comes from Fig. 4."
                     /gene="pilV"
                     /product="PilV"
                     /codon_start=1
                     /translation="IWTTAKVNFTTSTYNIGKNTRNLSIGVHAYCSWTYLNGAPFGGF
                     QQVYSDQNKVWYVNNYAWGNYESGGTITVTCLNLPGAGI"
     CDS             1712..1930
                     /partial
                     /note="Description: PilV variable region A;  variable
                     region C';  For the protein sequence (NCBI gibbsq 117391):
                      Method: conceptual translation supplied by author.  This
                     sequence comes from Fig. 4."
                     /gene="pilV"
                     /product="PilV"
                     /codon_start=1
                     /translation="RWSGGNKVNYSACKWYQSSVAMNHFIGGKSGGSIYYKPIQCPTG
                     FIMTGTRMYGIGDGVDEEHVDAYCCPFG"
     CDS             2213..2494
                     /partial
                     /note="Description: PilV variable region A;  variable
                     region D';  For the protein sequence (NCBI gibbsq 117392):
                      Method: conceptual translation supplied by author.  This
                     sequence comes from Fig. 4."
                     /gene="pilV"
                     /product="PilV"
                     /codon_start=1
                     /translation="TWRRASGSTVLTGKIANGQQIPLPSGFSASQCTWSVSNAENPHG
                     WKPNYFAGSVATYDANRIVKCGFYDEYNFYGGTHRTDLSGKCSYIVVCQ"
     CDS             3412..4536
                     /note="site-specific recombinase;  For the protein
                     sequence (NCBI gibbsq 117393):  Method: conceptual
                     translation supplied by author.  This sequence comes from
                     Fig. 4."
                     /gene="rci"
                     /product="Rci"
                     /codon_start=1
                     /translation="MFQKIKIRKMTISRALDKYLKTVSIHKKGHLQEFYRVNVIKRHP
                     IAERYMDDITTVDIANYRDQRLAQINPRTGRQITGNTVRLELALLSSLFNIARVEWGT
                     CRMNPVELVRKPKISSGRDRRLTSGEERRLSRYFKEKNQALYVIFHLALETAMRQGEI
                     LSLRWEHVDLQHGVAHLPTTKNGAPRDVPLSRKARNYLQMLPTQLNGNIFSYTSSGFK
                     SAWRTALQELKIENLHFHDLRHEAISRFFELGTLNVIEVAAISGHRSLNMLKRYTHLR
                     AYQLVSKLDARRKQTSKIAPYFVPYPATVENRNGQVVVTLSDFDLETSAATKEQAIFH
                     ASVLLLRTLAQAAQRGERVPTPGELPTNIDERVMICPLTN"
     CDS             677..1708
                     /partial
                     /note="For the protein sequence (NCBI gibbsq 117387):
                     Method: conceptual translation supplied by author.  This
                     sequence comes from Fig. 4."
                     /gene="pilV"
                     /product="PilV constant region"
                     /codon_start=1
                     /translation="MKKTDKGVSLLEVLLVIGIMVMVIPKVYENIENHLNNVRWQNAA
                     EHANTYNTAVRNYVADNASTLLAGSLPKTITPATLIQKGYLKSGFSESNFGQSYITGI
                     AKNSKTSRLEALTCSNGGQSLSEAGMRSVASMIEGLGGYINSSKQAIGAGGGWSDTPS
                     NYGLNCATGHIAMALVGADLQESDRLYRYSITNRPDLNRMHTAIDMNSNNLNNVGTLN
                     GNAAALSGDISARNGTFSGAISGNTATTNGDITSNNGWLVTKNSKGWMNSTYGGGWYM
                     SDSSWLRSVNNKGIYTGGQVKGGTVRADGRLYTGEYLQLEKTATAGASCSPNGLVGRD
                     STGAILSCQS"
BASE COUNT     1433 a   1161 c   1120 g   1344 t
ORIGIN      
        1 ctacaataaa tacatggtaa ggtaagttat gctaaacagc atatttaata cggcgatact
       61 ttgctgtgca aatatcgtct gtcaatgcta cgcctacaat gaagttaaat tccgtttaaa
      121 taaacttaat gtatcttata aaaccggaac agagaaatat tactgcctga tactgacaac
      181 gcttgccgtt ctgtatcttt cattaaatca gctatcactg attcaatcca taatagtgat
      241 tgttttctat gccttcctta cattaatggc ctgtatagac cttctttcat ttctactacc
      301 acgcctttat accgtgacat ttatcttctc cggcctgtta tatcagacat ggaataacaa
      361 tatattgtct ggtttgtttt gtgcaatgct gatgttcttc attatgctgt ttgtaagact
      421 ttactttgca tataaaaacg gaacggaaag tttcggcatg ggagatgtgc tacttattgc
      481 aggaactggc gtatggtttc caacgcctga gattgcctgt tcaattgtat ttattgccgt
      541 tatcggcgga attattttct tcactcttgg cggattaaac aagcaaaaaa aatatatccc
      601 attcggtccg ttcctttgtg gcggaatgtt tgtttactca ctcgtaccgg gaatattatt
      661 ctgaggatta agagcaatga aaaagacaga taaaggtgta tctctccttg aggtattact
      721 tgtcatcggc ataatggtga tggttatccc taaagtttat gaaaacattg aaaaccatct
      781 gaataatgtt cgctggcaga atgcagcaga acacgcaaat acctataaca ccgctgttcg
      841 taattatgtt gctgacaatg catcaacact acttgctggc tctttaccca aaacaatcac
      901 gccagcaaca ctcattcaga aaggctatct gaaatctggt ttttctgaat ccaattttgg
      961 acaaagctac attaccggta ttgcaaagaa cagcaaaaca tcccgtctgg aagccctgac
     1021 ctgtagtaat ggcggtcagt ccctgtcaga agccggaatg cgctcggtag caagcatgat
     1081 tgaagggctt ggcggctaca tcaacagcag caagcaggcg attggcgcgg gtggcggctg
     1141 gtctgacacg ccttcaaatt acgggctgaa ctgcgcgacc gggcatattg caatggcact
     1201 tgttggcgct gatttacagg aaagcgaccg tttgtatcgt tactccataa ccaatcgtcc
     1261 cgaccttaac aggatgcata cggcgattga tatgaacagc aacaatctga acaacgtcgg
     1321 cactttaaac ggcaacgccg ccgcgctctc cggcgatatc agcgcacgta acggtacgtt
     1381 ctctggcgca atttcaggaa atacagccac aaccaacggt gatatcactt cgaataatgg
     1441 ctggctggtc acaaaaaaca gcaaaggctg gatgaacagc acctatggtg gcggctggta
     1501 tatgtccgat tcgtcctggt tgcgcagcgt gaataataaa gggatttata ctggcggcca
     1561 ggtaaaaggt ggaactgtac gcgctgatgg acgcctctat acaggcgagt atttacagct
     1621 tgaaaaaaca gcaacggctg gcgcttcctg ctcccctaac gggcttgtag gccgcgacag
     1681 tacaggggcg atactttcgt gccaatccgg tcggtggtct ggtggcaata aagtgaacta
     1741 ctccgcctgt aagtggtatc aatcatctgt agcaatgaat cacttcattg gcgggaaaag
     1801 tggaggatct atctactaca aacccattca atgcccgacc ggatttatca tgacaggtac
     1861 acgcatgtat ggcattggtg atggcgttga tgaggaacac gttgatgctt attgttgccc
     1921 cttcggttaa atcccagcac caggaagatt caggcatgta acagtaattg tgccgccgga
     1981 ctcataattt ccccacgcat aattattcac ataccaaacc ttgttttggt cggagtatac
     2041 ctgctgaaac ccaccaaaag gcgcaccatt aaggtatgtc cacgaacagt acgcatgtac
     2101 gccaatgctc agatttcggg tgtttttccc gatattgtac gtactggtag tgaagttcac
     2161 ttttgccgtt gtccagatac cggattggca cggaggccag tgccaatccg gtacgtggag
     2221 aagggcatca ggtagcactg ttctgacagg aaaaatagca aatggacaac agatcccatt
     2281 gccatctggt ttttctgcca gccagtgtac ctggagtgtc agtaatgcag aaaatccgca
     2341 cgggtggaag ccgaattatt ttgcggggtc agttgcaacc tatgatgcca atcgaattgt
     2401 gaaatgcggc ttctatgacg aatacaactt ttatggcggt actcaccgaa ctgacctgtc
     2461 ggggaaatgt agttacatcg tggtttgcca gtgataagta tcgtcactgg caaatggcgt
     2521 aaactgtaat tgaaccattt tgatcttcgg tcgtgtcgca actggcagtc catgcattgt
     2581 tgcctgaagg catggaacgc gtgagccata taaatccggt atttgatctg cattgaccgc
     2641 ctccaccaat gactttcttg ccagatggac atgttgccgt tgctccaggc caacgccacg
     2701 cagtagctga agcggtaaca atttggctat cacctgcgcc aatttttttc cacgtaccgg
     2761 attggcactg gcctccgtgc caatccggtg tgtggagggc attaggtgga aagctcaagg
     2821 ttactcaact ttccagcact ggttacttgg ggcaatttga cttttgtgcc attgccagaa
     2881 tgggaaatgc tgaggattct cattactgcc aggtagtcga gagtccatct ggttcacgaa
     2941 aatggtacaa atacgagcac aagacaggtt gtattgcgtc ctgtgtaact ctcaattaag
     3001 tttggtatcc aaatactgaa aatacccctg agccacaaga gtagttttgg gccggataag
     3061 aggtgatctg ataagttgcg ccagcaggga cagcaaaaga gatgaatgct gtcttcccat
     3121 aagatgggtt attactcgca tttgtgctaa tcaatgcccc tgcaacgtat ccctgcaatc
     3181 tggatgtgtt agcacaatct cccccggcgg aaccaccatt ccctccagag gcgtaaacaa
     3241 acagcgtacc acttcccgta ttgcgaccag taaatgatcc tctgtgtgat ccaagattac
     3301 tgtaactacc gttagaggaa ccggaagttc tccacacacc ggattggcac ggtgatattg
     3361 catttcgaag caagaatctc taccttcccc ccttttttgt ctggagggga tatgtttcag
     3421 aaaatcaaaa ttcgcaaaat gacaatcagc cgggcgctgg acaaatacct gaagacagta
     3481 tccatccaca aaaaaggcca tctacaggag ttttatcgcg taaatgtgat taaacgtcac
     3541 cctattgctg aacgctatat ggatgacatt accactgttg atattgccaa ttatcgggat
     3601 caacggttgg cgcaaattaa cccccgaacg gggcgtcaaa taaccggaaa caccgtccgt
     3661 cttgaacttg ccctgttgtc atcattattc aatatcgccc gtgtggaatg gggaacatgc
     3721 cgcatgaatc ctgtcgaact ggtcagaaaa cccaaaatca gtagcgggcg cgatcgtcgc
     3781 ctgacttcag gagaagaacg ccgactttcg cggtatttca aagaaaaaaa tcaggctctt
     3841 tacgtcattt ttcatctggc gctggaaaca gcaatgcgac aaggtgaaat cttgtcattg
     3901 cgttgggaac acgtcgatct gcaacatggg gtagctcatc ttccaacaac caaaaacggt
     3961 gctccacgcg atgttccgct atccaggaaa gcccgtaatt acctgcaaat gctgccgaca
     4021 cagcttaacg gtaatatttt ttcttatacc tcttcgggat tcaaaagtgc ctggcgaaca
     4081 gcacttcagg aactgaagat agaaaacttg cattttcatg acctccgtca tgaggctatc
     4141 agccgatttt tcgaacttgg tacattgaac gtcattgagg ttgccgctat atcaggccat
     4201 cgctccctga acatgttgaa acgatacaca caccttcggg cttatcaact tgtcagtaaa
     4261 ctcgatgccc gacgcaagca aaccagcaaa atcgctccct attttgttcc ctacccggca
     4321 acggtcgaga acagaaacgg gcaagtcgtt gtgactctga gtgatttcga cctggaaaca
     4381 agcgcagcca caaaagaaca ggccatattt catgccagcg tgttgctgct acgaacactg
     4441 gcacaagccg cccagcgagg agaacgcgtc cctactcctg gcgaacttcc tacaaacatt
     4501 gatgaacggg taatgatttg tccattaaca aattagagga ttcacttatg atagaacaac
     4561 tacagagtga ttttttattc tggatattta tttacttcct gatagtaggt ttgcttatca
     4621 gaatatttca tcttaccaga aaaattaaat ccatcgaaaa aaacaaaatg ttcccagcgg
     4681 aggaatatgc tgtcctggac attgaacagc atatgtccaa taaatatatt gggcggctag
     4741 taacccgttt tatgaagaga actaatggtg cacctttgtt aattgtgttt ccagaatacg
     4801 ataaaggaga caacaatgaa gacaaaaatt ctccaccatt ccaacttgta aaactaacat
     4861 ccgacattaa caatattaaa gaaatttatt ccaatgatga tttaacctgt tttctgaata
     4921 atatttcggc acaaaaaaat ttatatacaa cgcactctgg acctagccag gcatggattg
     4981 aacatgcata tcctttgcaa caagtaacta ttctcttaca aggcactcgt catagtgaaa
     5041 gggaagatat aatcagtc
//