From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: D.pseudoobscura Adh gene for alcohol dehydrogenase
Date: 28 Dec 92 21:27:26 GMT
Distribution: bionet
Checksum: 61262 8

LOCUS       DROADHGN50   3474 bp ds-DNA             INV       01-DEC-1992
DEFINITION  D.pseudoobscura Adh gene for alcohol dehydrogenase
ACCESSION   X62230
KEYWORDS    ADH gene; alcohol dehydrogenase.
SOURCE      Drosophila pseudoobscura DNA.
  ORGANISM  Drosophila pseudoobscura
            Eukaryota; Animalia; Metazoa; Arthropoda; Uniramia; Insecta;
            Pterygota; Neoptera; Holometabola; Diptera; Brachycera;
            Cyclorrhapha; Schizophora; Drosophiloidea; Drosophilidae.
REFERENCE   1  (bases 1 to 3474)
  AUTHORS   Schaeffer,S.W. and Miller,E.L.
  TITLE     Estimates of gene flow in Drosophila pseudoobscura determined from
            nucleotide sequence analysis of the alcohol dehydrogenase region
  JOURNAL   Genetics 132, 471-480 (1992)
  STANDARD  full automatic
COMMENT     *source: strain=PS101; *source: isolate=Stemwinder Provincial Park,
            British Columbia, *source: Canada; *source: chromosome=IV section
            88;
               From EMBL    entry DPADHGN50;  dated 16-OCT-1992.
FEATURES             Location/Qualifiers
     mRNA            join(40..119,915..1020,1084..1488,1549..1964)
                     /note="adult mRNA (Adh)"
     mRNA            join(882..1020,1084..1488,1549..1964)
                     /note="larval mRNA (Adh)"
     promoter        8..14
                     /note="adult promoter (Adh)"
     prim_transcript 40..1964
                     /note="adult primary transcript (Adh)"
     exon            40..119
                     /note="adult exon 1a"
     intron          120..914
                     /note="adult intron"
     promoter        846..853
                     /note="larval promoter (Adh)"
     prim_transcript 882..1964
                     /note="larval primary transcript (Adh)"
     exon            882..1020
                     /note="larval exon 1"
     exon            915..1020
                     /note="adult exon 1b"
     intron          1021..1083
                     /number=1
     exon            1084..1488
                     /number=2
     intron          1489..1548
                     /number=2
     exon            1549..1964
                     /number=3
     polyA_signal    1941..1946
     polyA_site      1964
     CDS             join(928..1020,1084..1488,1549..1815)
                     /EC_number="1.1.1.1"
                     /gene="Adh"
                     /product="alcoholdehydrogenase"
                     /codon_start=1
                     /translation="MSLTNKNVVFVAGLGGIGLDTSRELVKRNLKNLVILDRIDNPAA
                     IAELKAINPKVTITFYPYDVTVPVAETTKLLKTIFAQVKTIDVLINGAGILDDHQIER
                     TIAVNYTGLVNTTTAILDFWDKRKGGPGGIICNIGSVTGFNAIYQVPVYSGSKAAVVN
                     FTSSLAKLAPITGVTAYTVNPGITKTTLVHKFNSWLDVEPRVAEKLLEHPTQTSQQCA
                     ENFVKAIELNKNGAIWKLDLGTLEPITWTQHWDSGI"
BASE COUNT      972 a    796 c    793 g    913 t
ORIGIN      
        1 cgtagtgtat ttaaggcact gcacatgtcg aggaccacga ttattgtcta cagtgcagtc
       61 agtagttgtc aggtgcagca ggcggaccag catttcttta aatctactca atcgatcaag
      121 taagtaactg taatccaagt acagtcggcc catcccactc ggttctgccg taactcaaga
      181 aatttcttga tagcgcagtt ccagttgtaa aacgtcgctt aaaaaaacat ctcgttggag
      241 cttgggttga aaaacgttcg agttgggcgt agaaaagttc aaatataaaa tatcaaaaaa
      301 gagaaaatat cttcccaaag cagctgtcca ttttttgtta aagtgataga tcaaaaattc
      361 gtatgacgta cattcctagt gcacttcaca caaacaattt cctcaattct aattctacaa
      421 gaagcacttt tggctcttgt ccagaaaaaa caatttgttt acacttgtcg gatgataaca
      481 gaggcggacc gacggctaag taacataaca gtcgcctgtt aagtaatgcc ctcactctct
      541 gttagatggc cgcgcgtgtc gactccgata gaccagcaga cacgcagtag ttttcctcaa
      601 gaacctgaac tctaaacata gacataattt acgatacctt gagactcaaa taaaaataca
      661 agtatgagtg gtattctatt cctgattaga ctcgcacaca cacacgtacg agtattaaca
      721 taaatttgcc ggcaaataac caaaacaaaa cgaacgcaaa cgatccgaac acgtaatgcg
      781 agagataaga aacagaaaag ctcgacgtga aagaagcttc tggcggaagc tcttgccgaa
      841 ctccctataa atacgagaca gaaaaccaga ataaatataa taacaaactg ttgaattgtc
      901 ccatcgattc ccagatcaaa agacagaatg tctctcacca acaagaacgt cgttttcgtg
      961 gccggtctgg gcggcattgg cctggacacc agccgggagt tggtcaagcg taatctgaag
     1021 gtaagagaga acgaattcca tggaatctat ggaatcctaa atttaaaaat acattttttt
     1081 tagaacctgg tcatcctgga tcgcattgac aatccggctg ccattgccga actgaaggca
     1141 atcaatccca aggtgaccat caccttctat ccctacgatg tgactgtgcc cgtcgctgag
     1201 accaccaagc tcctgaagac catctttgcc caggtgaaga caatcgatgt cctgatcaac
     1261 ggtgctggca tcctggacga tcatcagatc gagcgcacca ttgccgttaa ctacacgggc
     1321 ctggtcaaca ccaccacagc cattctggac ttctgggaca agcgcaaggg cggcccaggc
     1381 ggcatcattt gcaacattgg atccgtcacc ggtttcaatg ccatctacca ggtgcccgtt
     1441 tactccggct ccaaggcggc ggtggttaac tttacctcct ccctggcggt aagtaatcca
     1501 acgtttttcc tactccccaa aactaaatat ttctttgaaa tcccatagaa actggctccc
     1561 attactggtg tcactgctta cactgtcaat cctggcatca ctaagaccac tctggtccac
     1621 aaattcaact cgtggctgga tgtggagccc cgtgtggcgg agaagctgct cgagcatccc
     1681 acccagacct ctcagcagtg cgccgagaac tttgtgaagg ccattgagct gaacaagaac
     1741 ggtgccatct ggaagttgga tctgggcacc ttggagccca tcacatggac ccagcactgg
     1801 gactcgggca tctaaaccat cccagagact ctatgggaca tggcgtttta gcttttagtt
     1861 tctttttttc cactcaattg ttacgtatat acatacacat atggcaataa ggctgatttg
     1921 aaccccgttt tgaatatgat aatacaaaat tatatttgag aaatttcaac aaaatcgata
     1981 agagctatag ctctctttta attaaaaaaa actagataaa aacagcaatg gtcagtggtg
     2041 gtcagtggtc gttgtggcct ctatcgattt ccacacaaaa actttactgt ttagtaatag
     2101 aaaagaactt gagaaaggca gccaaaagaa tgtacgatct gacgggtaag catgtctgct
     2161 atgtggctga ctgcggtggc attgcactgg agaccagcaa ggttctcatg accaagaata
     2221 tagcggtgag tggtagagtg gtgtagagag tggagagtgg ttctggttct taaacaagtc
     2281 tctagtctct agtctctagt ctcttggctg ctgctggtag ctgtcgaaac taattaagtt
     2341 aatgactttt tcatgttttg tttttgtccg ctgacttttg ctgttcaaag ctacaattaa
     2401 gtttatgttt tgagtttgat ctacttttgc ttaagggaag tactttcgat tacggattgc
     2461 tggcatataa aaatacatac caaggaatgt gcatcatatc atattgattc tatcttgatt
     2521 ctttgcccag aaactggcca tccttcagag cgtggaaaac cccccggcca tcgctcagtt
     2581 acaatcgatt aagcacagca cacagatctt cttctggacc ttcgatgtga ccatggcccg
     2641 cgaggagatg aagaagtact tcgatgaggt catggtccag atggactaca tcgatgtact
     2701 gatcaatggg gccaccctgt gcgatgagcg gaacattgat gccaccatca atacgaatct
     2761 gaccggcatg atgaacaccg tggccactgt gctgccctat atggaccgta agatgggcgg
     2821 ttcgggtgga ctgattgtga atgtcacctc cgtcatagga ctggatccat cgccggtctt
     2881 ttgtgcgtac agtgcctcaa agtttggtgt gattgggttc acccgaagtc tagcggtgag
     2941 tgggcgagat cttagcttga aaatgctctc taatgtcttt cattctttcg caggatcccc
     3001 tgtattacac ccaaaatggt gtggctgtca tggccgtctg ctgtggtccc accaaagtgt
     3061 ttgtcgatcg ggaactgact gccttcctgg aatacggcca gtcctttgcc gatcgcctgc
     3121 gtcgtgcccc ctgccaatcg acggccgtct gcggccagaa tatagtaaat gccatcgaga
     3181 gatcggagaa cggacagatc tggattgccg acaagggcgg cctggagtcg gtggccctcc
     3241 actggtattg gcatatggcc gatcagtttg tgaactatat gcagagcact gacgatgagg
     3301 atcaggagtt ttttttgggc cagcgatgag gcatgccttg atccacggaa taaaacatca
     3361 aatgtgtcac aaaaagtagg caacttgttt tgtgtgtttg gaaatagatt tgaaatattt
     3421 tgaggtcata tgaataatga cataaatgag ggtgcctgaa agtatgccaa gttt
//