From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: S.cereviciae mitochondrial (nuclear encoded) isocitrate
Date: 29 Dec 92 21:28:52 GMT
Distribution: bionet
Checksum: 52206 4

LOCUS       YSCISODH     1229 bp ds-DNA             PLN       14-DEC-1992
DEFINITION  S.cereviciae mitochondrial (nuclear encoded) isocitrate
            dehydrogenase-1 (IDH1) gene, complete cds.
ACCESSION   M95203
KEYWORDS    dehydrogenase alpha-4-beta-4 subunit; isocitrate dehydrogenase-1.
SOURCE      Saccharomyces cerevisiae DNA.
  ORGANISM  Saccharomyces cerevisiae
            Eukaryota; Fungi; Eumycota; Hemiascomycetes; Endomycetales;
            Saccharomycetaceae.
REFERENCE   1  (bases 1 to 1229)
  AUTHORS   Cupp,J.R. and McAlister-Henn,L.
  TITLE     Cloning and characterization of the gene encoding the IDH1 subunit
            of NAD+-dependent isocitrate dehydrogenase from Saccharomyces
            cerevisiae
  JOURNAL   J. Biol. Chem. 267, 16417-16423 (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     misc_feature    132..133
                     /gene="IDH1"
                     /note="mitochondrial processesing site"
     CDS             100..1182
                     /gene="IDH1"
                     /product="isocitrate dehydrogenase-1 nuclear encoded"
                     /codon_start=1
                     /translation="MLNRTIAKRTLATAAQAERTLPKKYGGRFTVTLIPGDGVGKEIT
                     DSVRTIFEAENIPIDWETINIKQTDHKEGVYEAVESLKRNKIGLKGLWHTPADQTGHG
                     SLNVALRKQLDIYANVALFKSLKGVKTRIPDIDLIVIRENTEGEFSGLEHESVPGVVE
                     SLKVMTRPKTERIARFAFDFAKKYNRKSVTAVHKANIMKLGDGLFRNIITEIGQKEYP
                     DIDVSSIIVDNASMQAVAKPHQFDVLVTPSMYGTILGNIGAALIGGPGLVAGANFGRD
                     YAVFEPGSRHVGLDIKGQNVANPTAMILSSTLMLNHLGLNEYATRISKAVHETIAEGK
                     HTTRDIGGSSSTTDFTNEIINKLSTM"
BASE COUNT      368 a    263 c    256 g    342 t
ORIGIN      
        1 ctatttcaac agtaccttaa tattactggt aacaatcaag gttcattatt ctccctatcc
       61 tcattcttct cccttttcct ccataattgt aagagaaaaa tgcttaacag aacaattgct
      121 aagagaactt tagccactgc cgctcaggcg gaacgcaccc tacccaagaa gtatggcggt
      181 cgtttcaccg tcactttgat acctggtgac ggtgttggga aagaaatcac tgattcagtg
      241 agaaccattt ttgaggctga aaatatcccg atcgactggg aaactataaa cattaagcaa
      301 acagatcata aggaaggcgt ctatgaagct gttgagtctc taaagagaaa taagattggt
      361 cttaaggggc tatggcacac tcctgctgac caaacaggtc acggttcact aaacgttgct
      421 ttgcgtaaac aactagatat ctacgccaat gtggcccttt tcaaatcctt gaagggtgtc
      481 aagactagaa ttccagacat agatttgatt gtcattagag aaaacacgga gggtgagttc
      541 tcaggcctgg aacatgaatc cgtccctggt gtagtggaat ctttgaaagt tatgactaga
      601 cctaagacag aaaggatcgc cagatttgcc tttgacttcg ccaagaaata caacagaaag
      661 tctgtcacag ctgtgcataa ggcaaatatc atgaagttag gtgacggtct gttcagaaat
      721 ataataactg aaattggcca aaaagaatat cctgatattg acgtatcgtc catcattgtc
      781 gacaatgcct ccatgcaggc ggtggccaaa cctcatcaat ttgatgtcct agttacccct
      841 tcaatgtacg gtaccatctt aggcaacatt ggcgctgctt tgatcggtgg tccaggattg
      901 gtggcaggtg ccaactttgg cagggactat gctgtcttcg aaccaggttc cagacatgtt
      961 ggtttagata ttaaaggcca aaatgtggct aacccaactg ccatgatcct ttcctccacg
     1021 ttaatgttga accatttggg tttgaatgaa tatgctacta gaatctcaaa ggcagttcat
     1081 gaaacgatcg cagaaggtaa gcataccact agagatattg gtggttcctc ttctactact
     1141 gacttcacga atgaaatcat caacaaatta tctaccatgt aatgaaaaca attccccttt
     1201 tttttgttct gcaacttaag tgtgttcaa
//