From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Chicken mRNA for T-protein, complete cds.
Date: 12 Jan 93 05:46:00 GMT
Distribution: bionet
Checksum: 22852 4

LOCUS       CHKTP        1228 bp ss-mRNA            VRT       12-JAN-1993
DEFINITION  Chicken mRNA for T-protein, complete cds.
ACCESSION   D11162
KEYWORDS    T-protein; aminomethyl transferase; falota analogues binding;
            glycine cleavage system; glycinesynthase.
SOURCE      Gallus gallus (library: lambda gt11 and lambda gt10) liver cDNA to
            mRNA, clones CT5A, CT10 and CT5C.
  ORGANISM  Gallus gallus
            Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Aves;
            Neornithes; Neognathae; Galliformes; Phasianidae.
REFERENCE   1  (bases 1 to 1228)
  AUTHORS   Okamura-Ikeda,K., Fujiwara,K. and Motokawa,Y.
  TITLE     Molecular cloning of a cDNA encoding Chicken T-protein of the
            glycine cleavage system and expression of the functional protein in
            Eschrichia coli
  JOURNAL   J. Biol. Chem. 267, 18284-18290 (1992)
  STANDARD  full automatic
COMMENT     Submitted (11-May-1992) to DDBJ by: Yutaro Motokawa Institute for
            Enzyme Research The University of Tokushima 3 Kuramotocho Tokushima
            770 Japan Phone:  0886-31-3111 Fax:    0886-33-0771.
FEATURES             Location/Qualifiers
     transit_peptide 20..67
                     /function="mitochondrial target sequence"
     mat_peptide     68..1195
                     /product="T-protein"
                     /evidence="EXPERIMENTAL"
                     /codon_start=1
     polyA_signal    1210..1215
     polyA_site      1228
     CDS             20..1198
                     /product="T-protein"
                     /codon_start=1
                     /translation="MLRAGCRAALARRHLSSAPEGLKQTPLDALHRARGGRMVPFAGW
                     SLPVQYGRGHLESHLHTRRHCSLFDVSHMLQTRVYGRDRVRFLESLVVGDIAELRPGQ
                     GTLTLLTNERGDIVDDLIVTNTAEDHLYVVSNAGCADKDRAVMEGRAAELRAAGGDVH
                     LEVSGQRAAGVQGPSMAQVLQAGLPDDLTKLTFMTSTATTVFGVPGCRVTRCGYTGED
                     GVEISVPAGRAVELAERLLGCPEVWPAGLAARDSLRLEAGLCLYGNDIDESTTPVEAG
                     LLWTLGKRRRTAMDFPGAAIIMEQVKEKPKRKRVGLTSVGPPLRPPAAILGPEGTPVG
                     TVTSGCPSPSLGKNIAMGYVQAAHSRPGTTLTVEVRKKQHPALVTKMPFVPTHYYMAK
                     "
BASE COUNT      202 a    417 c    443 g    166 t
ORIGIN      
        1 gccgggccgg ggccggagga tgctgcgggc ggggtgccgc gccgcgttgg cccgacgcca
       61 cctgagctcg gcgccggagg ggctgaagca gacgccgctg gacgcgctgc accgggcccg
      121 cggcgggagg atggtgccgt tcgccggctg gagcctgccc gtgcagtacg gccgcgggca
      181 cctcgagtcg cacctgcaca cccggcggca ctgctcgctg ttcgacgtgt cccacatgct
      241 gcagacgcgg gtgtacggga gggaccgcgt ccgcttcctg gagagcctgg tggtggggga
      301 catcgccgag ctgcgcccgg gacagggcac cctgacgctg ctcaccaacg agcgcggcga
      361 catcgtggat gacctcatcg tcaccaacac ggccgaggac cacctctatg tggtgtccaa
      421 cgcgggctgc gccgacaagg accgagccgt catggagggc agagcagcag aactgagggc
      481 ggccggtggc gacgtgcacc tggaggtgtc cggacaacgc gctgctggcg tgcaagggcc
      541 ctccatggca caggtgctgc aggccgggct gccggatgac ctgaccaagc tgaccttcat
      601 gaccagcaca gccaccaccg tctttggcgt gccgggctgc cgtgtgacac gctgcggcta
      661 caccggcgag gacggtgtgg agatctcagt gcctgcaggg cgagcagtgg agctggccga
      721 gcggctgctg ggctgccccg aggtgtggcc ggcagggctg gcagcccggg acagcctgcg
      781 cctggaggcc gggctctgcc tctacggcaa cgacatcgat gagagcacca cgccagtgga
      841 ggctgggctg ctgtggaccc tggggaagcg ccggcgcaca gccatggact tccccggtgc
      901 agccatcatc atggagcagg tgaaggagaa gccaaagcgg aagcgtgtgg ggctgacatc
      961 ggtggggccc cccctccggc cgcctgccgc catcctgggc cccgagggca cacccgtggg
     1021 cacggtgacc agcggctgcc cctctccctc cctgggcaag aacatcgcca tgggctacgt
     1081 gcaggctgcg cacagccgcc ccggcaccac gctcaccgtc gaggtgagga agaagcagca
     1141 cccggcgctg gtcaccaaga tgcccttcgt gcccacacat tattacatgg ccaagtgaga
     1201 cccggcccca ttaaagcccc tcgctcat
//