From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: bdhA=butanol dehydrogenase I, bdhB=butanol dehydrogenase II
Date: 28 Jan 93 05:48:25 GMT
Distribution: bionet
Checksum: 03652 9

LOCUS       S118000      3893 bp    DNA             BCT       27-JAN-1993
DEFINITION  bdhA=butanol dehydrogenase I, bdhB=butanol dehydrogenase II
            [Clostridium acetobutylicum, ATCC 824, Genomic, 3 genes, 3893 nt]
ACCESSION   S49339 M96945 M96946
KEYWORDS    .
SOURCE      Clostridium acetobutylicum ATCC 824
  ORGANISM  Clostridium acetobutylicum
            Unclassified.
REFERENCE   1  (bases 1 to 3893)
  AUTHORS   Walter,K.A., Bennett,G.N. and Papoutsakis,E.T.
  TITLE     Molecular characterization of two Clostridium acetobutylicum ATCC
            824 butanol dehydrogenase isozyme genes.
  JOURNAL   J. Bacteriol. 174, 7149-7158 (1992)
  STANDARD  full automatic
COMMENT     This entry [NCBI gibbsq 118000] was created by the journal scanning
            component of NCBI/GenBank at the National Library of Medicine.
            This sequence comes from Fig. 1.
FEATURES             Location/Qualifiers
     CDS             1..333
                     /partial
                     /note="Description: orf1 5' of bdhA"
     CDS             724..1893
                     /note="mismatches(27[K->R],387[L->K]).  BDH I;  For the
                     protein sequence (NCBI gibbsq 118001):  Method: conceptual
                     translation supplied by author.  This sequence comes from
                     Fig. 1.
                     Author-given protein sequence is in conflict with the
                     conceptual translation"
                     /gene="bdhA"
                     /product="butanol dehydrogenase I"
                     /codon_start=1
                     /translation="MLSFDYSIPTKVFFGKGKIDVIGEEIKKYGSRVLIVYGGGSIKR
                     NGIYDRATAILKENNIAFYELSGVEPNPRITTVKKGIEICRENNVDLVLAIGGGSAID
                     CSKVIAAGVYYDGDTWDMVKDPSKITKVLPIASILTLSATGSEMDQIAVISNMETNEK
                     LGVGHDDMRPKFSVLDPTYTFTVPKNQTAAGTADIMSHTFESYFSGVEGAYVQDGIRE
                     AILRTCIKYGKIAMEKTDDYEARANLMWASSLAINGLLSLGKDRKWSCHPMEHELSAY
                     YDITHGVGLAILTPNWMEYILNDDTLHKFVSYGINVWGIDKNKDNYEIAREAIKNTRE
                     YFNSLGIPSKLREVGIGKDKLELMAKQAVRNSGGTIGSLRPINAEDVLEIFKLSY"
     CDS             2170..3342
                     /note="Authors translate GTG start codon as Val;
                     mismatch(233[A->E]).  BDH II;  For the protein sequence
                     (NCBI gibbsq 118003):  Method: conceptual translation
                     supplied by author.  This sequence comes from Fig. 1.
                     Author-given protein sequence is in conflict with the
                     conceptual translation"
                     /gene="bdhB"
                     /product="butanol dehydrogenase II"
                     /codon_start=1
                     /translation="VVDFEYSIPTRIFFGKDKINVLGRELKKYGSKVLIVYGGGSIKR
                     NGIYDKAVSILEKNSIKFYELAGVEPNPRVTTVEKGVKICRENGVEVVLAIGGGSAID
                     CAKVIAAACEYDGNPWDIVLDGSKIKRVLPIASILTIAATGSEMDTWAVINNMDTNEK
                     LIAAHPDMAPKFSILDPTYTYTVPTNQTAAGTADIMSHIFEVYFSNTKTAYLQDRMAE
                     ALLRTCIKYGGIALAKPDDYEARANLMWASSLAINGLLTYGKDTNWSVHLMEHELSAY
                     YDITHGVGLAILTPNWMEYILNNDTVYKFVEYGVNVWGIDKEKNHYDIAHQAIQKTRD
                     YFVNVLGLPSRLRDVGIEEEKLDIMAKESVKLTGGTIGNLRPVNASEVLQIFKKSV"
BASE COUNT     1419 a    512 c    730 g   1232 t
ORIGIN      
        1 gaattctcac ctaaagtaag ttcttccact ttaaaatcag tgcttctaat tttttttctt
       61 aaaagggcta catttgtggt taaagattca gtgaagccct ctctaggacc tcttattaca
      121 gtttcaacag ttggttctgt tatagctctt tcagggggtt ttccaatact tataataatt
      181 gctttacttt caccatctag gaataatgct atacttcctt ttaaaatgga caatataaca
      241 tcatccatgc ttttatatac atttttatca ttaacaggtc aaaaaattga ttttgtatat
      301 tcaaatatgt ttaaatgggg atggttattg taatcttctt ctataagttt ttttataaca
      361 gaggattcta ttacatcaga ttggataaga ttatttatgt agacaatcat tgcagaaaaa
      421 tttctattat tagctatttt aaattctcta atcgttaaat ctgagcaatt tgtaaataag
      481 gtttctatag tatgtttatt tgttttaagg ctagttgaaa ccgtcttcgt cgttattttt
      541 agatgcttct tctttattaa aaattttatt aaacaacgaa aaattcaccc cctctattta
      601 tttatataat agtagtttgc atgaaatttc gttgtttatt catattagat gcttgtatta
      661 aaataataaa atagtaaaat ataagtagac aaactataaa tctattacta ggaggtaaga
      721 agtatgctaa gttttgatta ttcaatacca actaaagttt tttttggaaa aggaaaaata
      781 gacgtaattg gagaagaaat taggaaatat ggctcaagag tgcttatagt ttatggcgga
      841 ggaagtataa aaaggaacgg tatatatgat agagcaacag ctatattaaa agaaaacaat
      901 atagctttct atgaactttc aggagtagag ccaaatccta ggataacaac agtaaaaaaa
      961 ggcatagaaa tatgtagaga aaataatgtg gatttagtat tagcaatagg gggaggaagt
     1021 gcaatagact gttctaaggt aattgcagct ggagtttatt atgatggcga tacatgggac
     1081 atggttaaag atccatctaa aataactaaa gttcttccaa ttgcaagtat acttactctt
     1141 tcagcaacag ggtctgaaat ggatcaaatt gcagtaattt caaatatgga gactaatgaa
     1201 aagcttggag taggacatga tgatatgaga cctaaatttt cagtgttaga tcctacatat
     1261 acttttacag tacctaaaaa tcaaacagca gcgggaacag ctgacattat gagtcacacc
     1321 tttgaatctt actttagtgg tgttgaaggt gcttatgtgc aggacggtat acgagaagca
     1381 atcttaagaa catgtataaa gtatggaaaa atagcaatgg agaagactga tgattacgag
     1441 gctagagcta atttgatgtg ggcttcaagt ttagctataa atggtctatt atcacttggt
     1501 aaggatagaa aatggagttg tcatcctatg gaacacgagt taagtgcata ttatgatata
     1561 acacatggtg taggacttgc aattttaaca cctaattgga tggaatatat tctaaatgac
     1621 gatacacttc ataaatttgt ttcttatgga ataaatgttt ggggaataga caagaacaaa
     1681 gataactatg aaatagcacg agaggctatt aaaaatacga gagaatactt taattcattg
     1741 ggtattcctt caaagcttag agaagttgga ataggaaaag ataaactaga actaatggca
     1801 aagcaagctg ttagaaattc tggaggaaca ataggaagtt taagaccaat aaatgcagag
     1861 gatgttcttg agatatttaa aaaatcttat taatagaaac tgtagaggta tttttataat
     1921 ttaaaagatg ttaaagagtg aggagtaatt ttgttctaac gcctcactct tttcatttta
     1981 tgattaaatg tatgctgatt tacgctaact taaatcctaa ataataacct aatgttaata
     2041 ttttgtaaca aatggataaa agcgtaaaaa tattattgta ataattttaa gtaggtttaa
     2101 aatatatata atgtagaagc attcctacat tatattattt aaataataat ctaaacagga
     2161 ggggttaaag tggttgattt cgaatattca ataccaacta gaattttttt cggtaaagat
     2221 aagataaatg tacttggaag agagcttaaa aaatatggtt ctaaagtgct tatagtttat
     2281 ggtggaggaa gtataaagag aaatggaata tatgataaag ctgtaagtat acttgaaaaa
     2341 aacagtatta aattttatga acttgcagga gtagagccaa atccaagagt aactacagtt
     2401 gaaaaaggag ttaaaatatg tagagaaaat ggagttgaag tagtactagc tataggtgga
     2461 ggaagtgcaa tagattgcgc aaaggttata gcagcagcat gtgaatatga tggaaatcca
     2521 tgggatattg tgttagatgg ctcaaaaata aaaagggtgc ttcctatagc tagtatatta
     2581 accattgctg caacaggatc agaaatggat acgtgggcag taataaataa tatggataca
     2641 aacgaaaaac taattgcggc acatccagat atggctccta agttttctat attagatcca
     2701 acgtatacgt ataccgtacc taccaatcaa acagcagcag gaacagctga tattatgagt
     2761 catatatttg aggtgtattt tagtaataca aaaacagcat atttgcagga tagaatggca
     2821 gaagcgttat taagaacttg tattaaatat ggaggaatag ctcttgagaa gccggatgat
     2881 tatgaggcaa gagccaatct aatgtgggct tcaagtcttg cgataaatgg acttttaaca
     2941 tatggtaaag acactaattg gagtgtacac ttaatggaac atgaattaag tgcttattac
     3001 gacataacac acggcgtagg gcttgcaatt ttaacaccta attggatgga gtatatttta
     3061 aataatgata cagtgtacaa gtttgttgaa tatggtgtaa atgtttgggg aatagacaaa
     3121 gaaaaaaatc actatgacat agcacatcaa gcaatacaaa aaacaagaga ttactttgta
     3181 aatgtactag gtttaccatc tagactgaga gatgttggaa ttgaagaaga aaaattggac
     3241 ataatggcaa aggaatcagt aaagcttaca ggaggaacca taggaaacct aagaccagta
     3301 aacgcctccg aagtcctaca aatattcaaa aaatctgtgt aaacctaccg gggtttgggc
     3361 gtcagattat attcatgaac tccaagaaag cagtatgcta gcaaagaaat aaaactcaaa
     3421 gcagagagaa aatttagaca ttcaactata aataaaaaat accccccaaa gcattaatat
     3481 cttggggagt attttttatt ttgaagtatt ctgttcagct aaatattctt ctaaggtaat
     3541 acctctgttc ataatttctt gtgaggagga agaccgatat atcttacatg ccatggctca
     3601 aaattatact ttgttatgtt ttctttatcc ttaggatatc ttattatgaa accatattta
     3661 ccacaatttt gttgaagcca tttataagaa tttgtattca taaatccatc atctaaagaa
     3721 gagtattcgg ttgatagtaa gtccattgcc aatccagttt gatgctcact tgtaccaggt
     3781 tcagctacat atttatcagc ttcggctttt ccgtctcgtg ctcatttttc attatataat
     3841 ttttgctgat acgaataagg tctataacct gaaacagcta gaagtgtaag acc
//