From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Pseudomonas aeruginosa pyrophosphate selective porin (OprO) gene,
Date: 10 Sep 92 10:35:27 GMT
Distribution: bionet
Checksum: 09760    12

LOCUS       PSEOPRO      3726 bp ds-DNA             BCT       10-SEP-1992
DEFINITION  Pseudomonas aeruginosa pyrophosphate selective porin (OprO) gene,
            complete cds and phosphate-selective porin (OprP) gene, complete
            cds.
ACCESSION   M86648 X53313 Y00553
KEYWORDS    oprP gene; outer membrane protein; pho box;
            phosphate starvation inducible regulon; phosphate transport;
            polyphosphate selective porin; porin; porin P.
SOURCE      Pseudomonas aeruginosa DNA.
  ORGANISM  Pseudomonas aeruginosa
            Prokaryotae; Gracilicutes; Scotobacteria; Pseudomonadaceae.
REFERENCE   1  (sites)
  AUTHORS   Siehnel,R.J., Worobec,E.A. and Hancock,R.E.
  TITLE     Regulation of components of the Pseudomonas aeruginosa
            phosphate-starvation-inducible regulon in Escherichia coli
  JOURNAL   Mol. Microbiol. 2, 347-352 (1988)
  STANDARD  full staff_review
REFERENCE   2  (sites)
  AUTHORS   Hancock,R.E., Martin,N.L. and Siehnel,R.J.
  TITLE     Sequence and relatedness in other bacteria of the Pseudomonas
            aeruginosa oprP gene coding for the phosphate-specific porin P
  JOURNAL   Mol. Microbiol. 4, 831-838 (1990)
  STANDARD  full staff_review
REFERENCE   3  (sites)
  AUTHORS   Siehnel,R.J., Benz,R., Egli,C. and Hancock,R.E.
  TITLE     Overexpression in Escherichia coli and functional analysis of a
            novel PPi-selective porin, OprO, from Pseudomonas aeruginosa
  JOURNAL   J. Bacteriol. 174, 471-476 (1992)
  STANDARD  full staff_review
REFERENCE   4  (bases 1 to 3726)
  AUTHORS   Siehnel,R.J., Egli,C. and Hancock,R.E.
  TITLE     Polyphosphate-selective porin OprO of Pseudomonas aeruginosa:
            Expression, purification and sequence
  JOURNAL   Mol. Microbiol. 6, 2319-2326 (1992)
  STANDARD  full staff_review
COMMENT     Siehnel, R.J. et al. (1988) Mol. Microbiol. 2, 347-352 reports base
            pairs 2017 through 2318.  Siehnel, R.J., Egli, C., and Hancock,
            R.E.W. (1992) reports base pairs 1 through 2318.  Siehnel, R.J. et
            al. (1990) Mol. Microbiol. 4, 831-838 reports base pairs 2017
            through 3726.
FEATURES             Location/Qualifiers
     repeat_region   195..235
                     /rpt_type=inverted
                     /rpt_unit=(195..213)
                     /note="putative"
                     /citation=[4]
     misc_signal     213..252
                     /function="regulatory signal (PhoB binding site)"
                     /phenotype="Induced by phosphate limitation as part of Pho
                     Regulon"
                     /standard_name="Pho Box"
                     /evidence=EXPERIMENTAL
                     /citation=[4]
                     /citation=[1]
     protein_bind    213..252
                     /function="transcription activation under phosphate
                     limitation"
                     /bound_moiety="PhoB"
                     /standard_name="Pho Box"
                     /note="putative"
                     /citation=[4]
                     /citation=[1]
     -10_signal      263..268
                     /note="putative"
     RBS             362..366
     CDS             376..1692
                     /function="pyrophosphate-selective porin (outer membrane
                     protein)"
                     /product="OprO"
                     /gene="oprO"
                     /evidence=EXPERIMENTAL
                     /codon_start=1
                     /translation="MIRKHSLGFVASALALAVSAQAFAGTVTTDGADIVIKTKGGLEV
                     ATTDKEFSFKLGGRLQADYSRFDGFYTKNGNTADAAYFRRAFIELGGTAYKDWKYQIN
                     FDLSHNTGSSDNGYFDEASVTYTGFNPVNLKFGRFDPDFGLEKATSSKWVTAPERNAA
                     YELADWINTHQDGMGAQVNSTLADMAYLSAGVSAKDADDSDGDSVKQFNFRGVFAPMH
                     EAGNVLHVGVNYAYRDLDDTAFDSRIRPRLGMRGIATSGGNDAGDNGNRATFGGVSNS
                     PAGSYKDDSVWGLEGAWAMGPFSAQAEYLARKLKADDNAYKDIKAKGYYAQLAYTLTG
                     ESRQYKLEGAKFDSVKPENKEIGAWEVFYRYDNIKVEDDNVVADTATREVGDTKAKAH
                     NLGVNWYVNDAVKISAAYVKAKTDKITNNNGDDDGDGFVTRLQYVF"
                     /citation=[3]
                     /citation=[4]
     sig_peptide     376..447
                     /gene="oprO"
                     /codon_start=1
     mat_peptide     448..1692
                     /note="pyrophosphate-selective porin (outer membrane
                     protein)"
                     /product="OprO"
                     /gene="oprO"
                     /codon_start=1
     terminator      1721..1745
     misc_signal     2008..2047
                     /function="regulatory signal (PhoB binding site)"
                     /phenotype="induced by phosphate limitation as part of pho
                     regulon"
                     /standard_name="Pho Box"
                     /note="putative"
                     /citation=[4]
                     /citation=[1]
     protein_bind    2008..2047
                     /function="regulates expression as part of pho regulon"
                     /bound_moiety="PhoB"
                     /standard_name="Pho box"
                     /evidence=EXPERIMENTAL
                     /citation=[4]
                     /citation=[1]
     promoter        2058..2063
                     /note="putative"
     stem_loop       2072..2114
                     /function="mRNA stability or regulatory feature"
                     /note="putative"
                     /citation=[4]
     RBS             2137..2141
                     /note="putative"
                     /citation=[4]
                     /citation=[1]
     CDS             2151..3473
                     /function="phosphate-specific porin (outer membrane
                     protein) of pho reg"
                     /standard_name="porin"
                     /product="OprP"
                     /gene="oprP"
                     /evidence=EXPERIMENTAL
                     /codon_start=1
                     /translation="MIRRHSCKGVGSSVAWSLLGLAISAQSLAGTVTTDGADIVIKTK
                     GGLEVATTDKEFSFKLGGRLQADYGRFDGYYTNNGNTADAAYFRRAYLEFGGTAYRDW
                     KYQINYDLSRNVGNDSAGYFDEASVTYTGFNPVNLKFGRFYTDFGLEKATSSKWVTAL
                     ERNLTYDIADWVNDNVGTGIQASSVVGGMAFLSGSVFSENNNDTDGDTVKRYNLRGVF
                     APLHEPGNVVHLGLQYAYRDLEDSAVDTRIRPRMGMRGVSTNGGNDAGSNGNRGLFGG
                     SSAVEGLWKDDSVWGLEGAWALGAFSAQAEYLRRTVKAERDREDLKASGYYAQLAYTL
                     TGEPRLYKLDGAKFDTIKPENKEIGAWELFYRYDSIKVEDDNIVVDSATREVGDAKGK
                     TRALGVNWYANEAVKVSANYVKAKTDKISNANGDDSGDGLVMRLQYVF"
                     /citation=[3]
                     /citation=[4]
                     /citation=[1]
     sig_peptide     2151..2237
                     /note="putative"
                     /codon_start=1
                     /citation=[3]
                     /citation=[4]
                     /citation=[1]
     mat_peptide     2238..3473
                     /note="phosphate-specific porin"
                     /product="OprP"
                     /gene="oprP"
                     /evidence=EXPERIMENTAL
                     /codon_start=1
                     /citation=[3]
                     /citation=[4]
                     /citation=[1]
     terminator      3480..3507
BASE COUNT      770 a   1202 c   1096 g    658 t
ORIGIN
        1 aagcttcgcc atcgtcaaca acggcatcga gttcgccgcg acctacctgg tgatgttgct
       61 cgcgctgttc ttcggcggcg ccggcaagtg gctttctgct gacttctgga ttgcgcaaaa
      121 attgcgctga gatttctacc catatcgaat aacccgaaag gcccgtagta gagggctttt
      181 cgcgttattc gcattggaga taaaaagttc ctctgtaatg aaacatttat ctccatgcaa
      241 tttggctgca atctgccatc gccaagattc ccccaacaca aaggggccgc cttacggcca
      301 gcgcggaaca aggggcgcgc tgtagccagg cactcgcgtg ttgtcaatgc acacaccatt
      361 aaggggaaat cttcgatgat ccgtaagcac tcgctcggct tcgttgccag cgctctggct
      421 ctggccgtat ctgcccaggc gttcgccggt accgtgacca ccgacggtgc cgatatcgtg
      481 atcaagacca agggtggcct cgaagtcgcc accaccgaca aggaattcag cttcaagctg
      541 ggcggtcgcc tgcaggccga ctacagccgt ttcgacggtt tctataccaa gaatggcaac
      601 accgccgacg ccgcctactt ccgccgcgcc ttcatcgaac tcggcggcac cgcgtacaag
      661 gactggaaat accagatcaa cttcgaccta tcgcacaaca ccggcagctc cgacaacggc
      721 tatttcgacg aagcttcggt cacctacacc ggcttcaacc cggtcaacct gaagttcggt
      781 cgcttcgacc ccgacttcgg cctggaaaag gccaccagct ccaagtgggt gaccgctccc
      841 gagcgtaacg ccgcctacga actggccgac tggatcaaca cccaccagga cggcatgggc
      901 gcccaggtca actcgaccct cgccgacatg gcctacctgt ccgccggcgt atccgccaag
      961 gacgccgacg acagcgacgg cgacagcgtc aagcagttca acttccgcgg cgtgttcgcg
     1021 ccgatgcacg aagccggcaa tgttctgcat gtcggcgtga actatgccta ccgcgacctc
     1081 gacgacaccg ccttcgactc gcgcatccgt ccgcgcctgg gcatgcgcgg catcgccacc
     1141 agcggcggca acgacgccgg tgacaacggc aaccgcgcga ccttcggcgg tgtctccaac
     1201 tcgccggccg gttcctacaa ggacgatagc gtctggggcc tggaaggcgc ctgggcaatg
     1261 ggcccgttct cggcccaggc cgaatacctg gcacgcaagc tgaaggccga tgacaacgcc
     1321 tacaaggaca tcaaggccaa gggttactac gcgcaactgg cctacaccct gaccggcgag
     1381 tcccgccagt acaagctgga aggtgccaag ttcgactccg tcaagccgga aaacaaggaa
     1441 atcggcgcct gggaagtgtt ctaccgctac gacaacatca aggtggaaga cgacaacgtg
     1501 gtcgccgata ccgccactcg cgaagtcggc gacaccaagg ccaaggccca caacctgggc
     1561 gtgaactggt acgtcaacga tgcggtgaag atcagcgcgg cctacgtcaa ggcgaagacc
     1621 gacaagatca ccaacaacaa tggcgacgat gacggcgacg gcttcgtgac ccgtttgcag
     1681 tacgtgttct aagaagcgac aacgatctgc tcctgttttc ggcctcgctc cggcgaggtc
     1741 tttttaatcg cttccttacc cggttgtggc gatgttggca gcctgcaacc gggtctttct
     1801 ttcgctacag gtctacccgc aggtactggc aggaatatcc gaaagccaga cggttatcgg
     1861 cagaaactct tggttttcat tgcagtcatg caatcttcgc aaggttgccg caatatatga
     1921 aacaaactgt tacatctgtc gcccggacct gatgcctcta ggcctgcgcc cttatccgac
     1981 ggcctcatcc aggcgctatc cggctgcctg tcacaaaagc tttcgcgctt tgcagtctcg
     2041 ctgtcacaac ccatccagat gatccccctc cagcagcgcc ggccggcgac agaaccggcc
     2101 gtttccgcgc tgctcaagac cattcgaaat ccgaacaggg gacttacctg atgattcgca
     2161 gacactcgtg caaaggggtg gggagcagtg ttgcctggag tttgctgggc ctggcgattt
     2221 ccgcgcagag cctggccggg accgtgacca ccgacggtgc cgacatcgtg atcaagacca
     2281 agggcggcct cgaagtcgcc accaccgaca aggaattcag cttcaagctc ggcggccgcc
     2341 tgcaggccga ctacggccgc ttcgacggct actacacgaa caacggcaat accgccgatg
     2401 ccgcctactt ccgccgcgcc tacctggagt tcggcggcac cgcctaccgg gactggaagt
     2461 accagatcaa ctacgacctg tcgcgcaacg tcggcaacga cagcgccggc tactttgacg
     2521 aagcttcggt cacctatacc ggcttcaacc cggtcaacct gaagttcggc cgcttctaca
     2581 ccgacttcgg cctggaaaag gccaccagct ccaaatgggt caccgcgctg gagcgcaacc
     2641 tcacctacga catcgccgac tgggtcaacg acaacgtcgg taccggtatc caagccagct
     2701 cggtggtggg cggcatggct ttcctctctg gcagcgtgtt cagcgagaac aacaacgata
     2761 ccgacggcga cacggtcaag cgctacaacc tgcgcggcgt gttcgcgccg ctgcacgagc
     2821 cgggcaacgt ggtgcacctg ggcctgcaat acgcctatcg cgacctggag gacagcgcgg
     2881 tggatacccg gatccgcccg cgcatgggca tgcgcggcgt ctccaccaat ggcggcaacg
     2941 atgccggcag caatggcaac cgcggtctgt tcggcggcag ttcggcagtc gaggggctgt
     3001 ggaaggacga ctcggtctgg ggcctggaag gcgcctgggc gctgggtgcc ttctcggccc
     3061 aggccgagta cctgcggcgc acggtcaagg ccgagcgcga ccgcgaggat ctcaaggcct
     3121 ccggctatta cgcgcaactg gcctacaccc tcaccggcga gccgcgcctc tacaagctgg
     3181 acggcgccaa gttcgacacc atcaagccgg agaacaagga aatcggcgcc tgggagctgt
     3241 tctaccgcta cgactcgatc aaggtcgagg atgacaacat cgtcgtcgac agcgccaccc
     3301 gcgaggtcgg cgacgccaag ggcaagaccc gtgccctggg cgtcaactgg tacgccaacg
     3361 aggcggtgaa ggtttccgcc aactacgtca aggcgaagac cgacaagatc agcaatgcca
     3421 acggcgacga tagcggcgat ggcctggtga tgcgcctgca gtacgtgttc tgaggcgctc
     3481 gggccccgtc cggcgggcgg ggctcgtttc cgcaggatgt tacccgcgtc atgaaattgc
     3541 ctggtggatg tccgggcggg ccgcccagct tgacccaggg cacagatcgc atggcggcat
     3601 ctggctatgc tggtccctcg gaaacgctct gggagggact gatgatcggc tggggacgtg
     3661 cctgtattgg cgtcgggggg ctgctgttgc tgggccattg ctggggggcg ccctgctgct
     3721 cgcgga
//