From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: K.pneumoniae plasmid pGSH500 replicon DNA
Date: 28 Jul 92 10:07:13 GMT
Distribution: bionet
Checksum: 22719    10

LOCUS       KPNPGSH500   2686 bp ds-DNA             BCT       28-JUL-1992
DEFINITION  K.pneumoniae plasmid pGSH500 replicon DNA
ACCESSION   Z11775
KEYWORDS    repB gene; replication protein.
SOURCE      Klebsiella pneumoniae DNA.
  ORGANISM  Klebsiella pneumoniae
            Prokaryotae; Gram-negative facultatively anaerobic rods;
            Enterobacteriaceae.
REFERENCE   1  (sites)
  AUTHORS   Da Silva Tatley,F.M., Elisha,B.G. and Steyn,L.M.
  TITLE     Dual oriv regions in a mutliresistant conjugative plasmid
  JOURNAL   Plasmid 25, 235-236 (1991)
  STANDARD  full staff_review
REFERENCE   2  (bases 1 to 2686)
  AUTHORS   Steyn,L.M.
  JOURNAL   Unpublished (1992)
  STANDARD  full staff_review
REFERENCE   3  (bases 1 to 2686)
  AUTHORS   Da Silva Tatley,F.M. and Steyn,L.M.
  TITLE     Characterisation of a replicon of the conjugative, multiple
            drug-restance, atypical promiscuous plasmid, pGSH500, with features
            of both the mini-replicon of pCU1 and and the ORI-2 of F
  JOURNAL   J. Mol. Biol. (1992) In press
  STANDARD  full staff_review
COMMENT     *source: strain=APP; *source: stage=adult; *source: tissue=blood
            culture;
            From EMBL    entry KPPGSH500;  dated 05-JUN-1992.
FEATURES             Location/Qualifiers
     CDS             complement(257..893)
                     /pseudo
                     /function="unknown"
                     /product="unknown polypeptide"
                     /codon_start=3
                     /citation=[1]
                     /citation=[2]
                     /citation=[3]
     misc_feature    270..576
                     /function="cis-ori of PFDT210"
                     /standard_name="cis-ori"
                     /note="region with 80% identity with thecorresponding
                     region in the ORI-2 of mini-F"
                     /product="none"
                     /evidence=EXPERIMENTAL
                     /citation=[1]
                     /citation=[2]
                     /citation=[3]
     misc_feature    380..393
                     /function="DNA melting site"
                     /standard_name="primosome site"
                     /note="identical to the RRIA site of COLE1 and F"
                     /product="RRIAsite"
                     /citation=[1]
                     /citation=[2]
                     /citation=[3]
     misc_feature    409..424
                     /function="DNA melting"
                     /standard_name="primosome site"
                     /note="identicalto the RRIA sites in COLE1 and F"
                     /product="RRIA site"
                     /citation=[1]
                     /citation=[2]
                     /citation=[3]
     CDS             complement(412..937)
                     /pseudo
                     /function="unknown"
                     /product="unknownpolypeptide"
                     /codon_start=1
                     /citation=[1]
                     /citation=[2]
                     /citation=[3]
     misc_feature    478..723
                     /function="minimal region required in cis forplasmid
                     replication"
                     /standard_name="cis-ori"
                     /evidence=EXPERIMENTAL
                     /citation=[1]
                     /citation=[2]
                     /citation=[3]
     misc_feature    478..2281
                     /function="basic replicon of PFDT210"
                     /standard_name="beta replicon"
                     /evidence=EXPERIMENTAL
                     /citation=[1]
                     /citation=[2]
     protein_bind    491..507
                     /function="bacterialchromosome and plasmid DNA
                     replication"
                     /bound_moiety="DNA A"
                     /note="two overlapping DNA A binding sites containedwithin
                     the cis-ori region of the plasmid PFDT210"
                     /citation=[1]
                     /citation=[2]
                     /citation=[3]
     CDS             673..1560
                     /function="confers POLA independence on plamidPGSH500"
                     /note="AAsequence has 58% identity with the product
                     ofORF239 of plasmid PCU1 and 31% with the repeat ofmini-F"
                     /product="replication protein"
                     /gene="REPB"
                     /evidence=EXPERIMENTAL
                     /codon_start=1
                     /translation="MGCASTSYGKEVKFSSQLSLVDKVQIFPVDKYRDSLQFLFSLSL
                     WVGKNLPIGIEMDTQALLPATKTFKKRASIKQSNELTEAAYYLPLQAKRVLWLCLMQA
                     YFNDSQEDDSDVLPLFKISVSDYVKYFNVATSVASRDVKAGVNALGESTVTFYPKEGE
                     FEEVKRPWLAEAGMKRGRGSWQIEFNYKVMPFLVGLTSQFTTYSLYDCGQLNSVRVIR
                     LYESLCQFRSTGVWITTHDWLCERFMLPASQKNNIAEMKRTFLEPALKKINEKTPLKV
                     SYKTEEDGRLLFNFLDGKQ"
                     /citation=[1]
                     /citation=[2]
     protein_bind    732..740
                     /function="bacterialchromosome and plasmid dna
                     replication"
                     /bound_moiety="DNA A"
                     /note="DNA box in the inverted orientation to the twoother
                     dna boxes contained within the ORF295"
                     /citation=[1]
                     /citation=[2]
                     /citation=[3]
     repeat_region   1605..2281
                     /function="plasmid copy number andincompatability"
                     /rpt_type=tandem
                     /rpt_unit=2246..2281
                     /standard_name="COP/INC"
                     /note="hexanucleotidetgtccc also occurs in the iterons of
                     PCU1"
                     /evidence=EXPERIMENTAL
                     /citation=[1]
                     /citation=[2]
                     /citation=[3]
BASE COUNT      645 a    683 c    597 g    761 t
ORIGIN
        1 ccatgccgga agccgatgcg gtacgctgat gaaacaactt gagaggcgct aaaaagctgc
       61 attcgcggtt ctgtaacgtc aagctatggc gcgggagtgg aagacaggcg taaaaaaggc
      121 tactggctcg gccttagtta ggtaacctgt gatatcaaac tgctctctca gctaagactt
      181 acagtgtaaa tgcagtaatt tatctaattc gcgtagtctg gccagatagc cagcactcat
      241 tgccttaccg ctttcatcat tggctccact catcgtagtg ttttgatatt accagcccag
      301 atccagcaca gccagcagaa ctggacagga taggctgtat gtctaacttc tggctacaga
      361 tatgattgag ccagtagtgc tcgccgcagt cgagcgacag ggcgaagccg agtgagcgag
      421 gaagcaccag ggaacaaaac ttataaattc tgcttacgct caatgcctga aaaatcacct
      481 ccccctcggg ttatccactt atccacgggc atatttttat aaagtacttt tttattcttt
      541 ttctttattt tacatctttt agggagcctg aaagccgcgt acagtgcggc ctgagagggg
      601 gcctgtatgg gcaactggta aacataatat gggaaattaa tcacgtatca gtgggttttg
      661 tgtcaacaag ctatgggatg cgcatcaaca agctatggga aagaggtcaa gttcagttct
      721 caattatcac ttgtggataa agtgcagatt tttccggtgg ataagtatcg cgatagcttg
      781 caatttctgt tttcactgtc attatgggtg ggtaaaaatt tacccatagg cattgaaatg
      841 gatactcaag cactcttgcc agctactaaa acgtttaaaa aacgcgccag tataaagcaa
      901 tctaacgaat tgaccgaagc tgcatattac ctgcctcttc aggctaagcg tgtgctgtgg
      961 ttatgcctca tgcaggccta ttttaatgac agccaggaag atgactctga cgttttgccg
     1021 ctcttcaaaa tcagcgtttc cgattacgtc aaatacttca atgtcgctac gtctgtcgcc
     1081 agtcgtgatg tgaaggctgg cgtaaatgct ctgggcgaat caacggttac cttttatcca
     1141 aaagaaggtg agtttgaaga agttaaacgt ccgtggctgg ctgaagcagg aatgaagcga
     1201 ggccggggtt cctggcagat tgaatttaac tacaaggtca tgccattcct tgtcggctta
     1261 acctcccagt tcactaccta ttctctctat gactgtggcc agcttaatag tgttcgtgtc
     1321 atccggcttt atgaaagcct gtgtcagttc cggagcactg gtgtctggat caccacacat
     1381 gactggttat gtgaaaggtt tatgctccct gcctcgcaga aaaacaatat tgctgaaatg
     1441 aagcgtacct tcctggaacc tgctctgaag aaaatcaatg agaagacacc attaaaggta
     1501 agttataaaa ccgaagaaga cggtcgcttg ttgtttaatt ttcttgatgg aaaacaatga
     1561 catgcattgt gccccccccc gttttggtaa attgctgaga tatgggggga tattaaactt
     1621 gctgtttttc ccatagtaca tgcctaactt gacttttttc ccaccaaacg ttcgagctga
     1681 ccgccaagtt aacctgtccc cctaatccta gccgcacgta aagttgacgt gtttcccaca
     1741 gtccagctag ttatcaaagt tgacctgttt cccatagccc tgcccggccc ctacgttgac
     1801 gtgtttccca caatcctggc cgttcgccaa gttgacctgt ttcccacagt cctggccgtc
     1861 caccaagttg acctgtttcc cacaatcctg gccgtccgcc aagttgacct gtttcccaca
     1921 gtcctggccg tccgccaagt tgacctgttt cccacaatcc tggccgtccg ccaagttgac
     1981 ctgtttccca cagtcccggc cgcagccaag ttgacctgtt tcccacaatc ccggccgcag
     2041 ccaagttgac ctgtttccca cagtcctggc cgtccgccaa gttgacctgt ttcccacagt
     2101 cctggccgca gccaagttga cctgtttccc acagtcctgg ccgtccgcca agttgacctg
     2161 ttcccacagt cctggccgca gccaagttga cctgtttccc atagtcctgg ccgcccccca
     2221 agttgacctg tttcccatag tcctggccgc cccccaagtt gacctgtttc ccatagtcct
     2281 gcttgtccgc aaagtcgatc tgttctcaac tgagtatgga ttactattct gttacagtgc
     2341 gtaagagcta tctgcatgta tggtcctctc cgcttcctcg cctgctcgct cgctatgctc
     2401 ggtctcatgg ctgcggcgag cgttaccatc tctctgattg atatttttct aggccgatta
     2461 tgtcgttcga tagcaccttc aatgtaactg tctaagaaag agttaattga aatggcgaag
     2521 ggctcagatg gcgtacaatc ctatacggct atgcctttat aatgtattgc cattgtattt
     2581 acattgtaaa tatggtgtat tttgttgctt ttgctactac cacttttatg aacagtgaca
     2641 tctcgaattt ttgttattca ctttgtataa atatccagta atagtt
//