From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: F.pringlei ppcA1 gene for phosphoenolpyruvate carboxylase
Date: 11 Sep 92 10:03:45 GMT
Distribution: bionet
Checksum: 09506    15

LOCUS       FTRPPCA      8846 bp ds-DNA             PLN       11-SEP-1992
DEFINITION  F.pringlei ppcA1 gene for phosphoenolpyruvate carboxylase
ACCESSION   X64144
KEYWORDS    phosphoenolpyruvate carboxylase; ppcA1 gene.
SOURCE      Flaveria pringlei DNA.
  ORGANISM  Flaveria pringlei
            Eukaryota; Plantae; Embryobionta; Magnoliophyta; Magnoliopsida;
            Asteridae; Asterales; Asteraceae.
REFERENCE   1  (bases 1 to 8846)
  AUTHORS   Hermans,J. and Westhoff,P.
  TITLE     Homologous genes for the C4 isoform of phosphoenolpyruvate
            carboxylase in a C3-and a C4-Flaveria species
  JOURNAL   Mol. Gen. Genet. 234, 275-284 (1992)
  STANDARD  full automatic
COMMENT     *source: clone_library=lambda DASH Sau3A; *source: clone=lnFpppc65;
            From EMBL    entry FPPPCA;  dated 03-SEP-1992.
FEATURES             Location/Qualifiers
     CDS             join(2584..2751,3269..3660,3737..3821,3912..4138,
                     4226..4328,4407..4494,4576..4730,5456..6454,7024..7410,
                     8061..8360)
                     /EC_number="4.1.1.31"
                     /product="phosphoenolpyruvate carboxylase"
                     /gene="ppcA1"
                     /codon_start=1
                     /translation="MANRNLEKLASIDAQLRLLVPGKVSEDDKLIEYDALLLDKFLDI
                     LQDLHGEDLKEAVQECYELSAEYEGKHDPKKLEELGSVLTSLDPGDSIVIAKAFSHML
                     NLANLAEEVQIAYRRRIKLKRGDFADEANATTESDIEETFKKLVLKLNKSPEEVFDAL
                     KNQTVDLVLTAHPTQSVRRSLLQKHGRIRNCLAQLYAKDITPDDKQELDEALHREIQA
                     AFRTDEIRRTPPTPQDEMRAGMSYFHETIWKGVPKFLRRVDTALKNIGINERVPYNAP
                     LIQFSSWMGGDRDGKHPRVTPEVTRDVCLLARMMASNMYFSQIEDLMFEMSMWRCNSE
                     LRVRAEELYRTARRDVKHYIEFWKQVPPTEPYRVILGDVRDKLYNTRERSRHLLAHGI
                     SDIPEEAVYTNVEQFLEPLELCYRSLCDCGDRVIADGSLLDFLRQVSTFGLSLVKLDI
                     RQESDRHTDVLDAITQHLEIGSYREWSEEKRQEWLLAELSGKRPLFGSDLPKTEEVKD
                     VLDTFNVLAELPSDCFGAYIISMATSPSDVLAVELLQRECHVKHPLRVVPLFEKLADL
                     EAAPAAMARLFSIDWYRNRIDGKQEVMIGYSDSGKDAGRFSAAWQLYKAQEEIIKVAK
                     EFGVKLVIFHGRGGTVGRGGGPTHLAILSQPPDTIHGSLRVTVQGEVIEQSFGEEHLC
                     FRTLQRFCAATLEHGMNPPISPRPEWRELMDQMAVVATEEYRSIVFKEPRFVEYFRLA
                     TPELEYGRMNIGSRPSKRKPSGGIESLRAIPWIFAWTQTRFHLPVWLGFGAAFKHAIK
                     KDSKNLQMLQEMYKTWPFFRVTIDLVEMVFAKGDPGIAALNDKLLVSEDLWPFGESLR
                     ANYEETKDYLLKIAGHRDLLEGDPYLKQRIRLRDSYITTLNVCQAYTLKRIRDPNYHV
                     TLRPHISKEYAAEPSKPADELIHLNPTSEYAPGLEDTLILTMKGIAAGMQNTG"
     CAAT_signal     2433..2437
     TATA_signal     2456..2462
     prim_transcript 2491..>8360
                     /gene="ppcA1"
     exon            2491..2751
                     /number=1
     intron          2752..3268
                     /number=1
     exon            3269..3660
                     /number=2
     intron          3661..3736
                     /number=2
     exon            3737..3821
                     /number=3
     intron          3822..3911
                     /number=3
     exon            3912..4138
                     /number=4
     intron          4139..4225
                     /number=4
     exon            4226..4328
                     /number=5
     intron          4329..4406
                     /number=5
     exon            4407..4494
                     /number=6
     intron          4495..4575
                     /number=6
     exon            4576..4730
                     /number=7
     intron          4731..5455
                     /number=7
     exon            5456..6454
                     /number=8
     intron          6455..7023
                     /number=8
     exon            7024..7410
                     /number=9
     intron          7411..8060
                     /number=9
     exon            8061..>8360
                     /number=10
BASE COUNT     2748 a   1488 c   1598 g   3005 t      7 others
ORIGIN
        1 tctagagctg tcgacaatta accctcacta aagggaacga attcggatct ttcatatcca
       61 cgaaaaggca tgagttcttg ccacttgacc aaggagtgtt cgtagagccg tacttactaa
      121 aacaaacaaa aaactaaaac aaacataatc ttccataaaa gatgaatcca acactttttc
      181 ttttttgtca tgatacatat acatatgtat gtgtgtgtga atatatgcat atatattgag
      241 aaatggaaat taagaacaaa ggtattctct gtacatagag gcgttaacgg gtcaaatttg
      301 cgtgttaaag accaaatctg atacgacctt gaagattttc tatgaatttg gaaagtatgc
      361 atacagaaaa attatgtaag agacgtgaac ttgaaaatga cttgtgtgtt gatttttttg
      421 tcaccaagaa atcaaatata tataaaataa ttgcactcgt aagatagttc gtttattcat
      481 gttttaactt tttacataaa atacctaata agtttgagat ttaaaataaa ataactctta
      541 aaatttaatc agaataacca ggtgaactca tgaacacatc gtactgactt tacactcacc
      601 catttagctg agagtgtttg taattcgacc ctattttgta ttgtgtcaat aattcacata
      661 tctagctata tgcattaggt gtgaggagcc tcgaaatttt atgggagggt tttatatcaa
      721 gaatttttat gatcatatag ttttcttttg tatctatgag agctagtgag gttacgatag
      781 attaaaaaac cactgaaatc aatatttgaa tataagacct ttggtaaaag agaattaagt
      841 atttactact acatactttt gaaggctttc tcgtatttta cactgagaat tacatcgttg
      901 ctaacttttt tgtataatat cttacataaa ttacttatat tttaaaagac atagtaagaa
      961 tcatttaagc ataaagtaca atcaatcaat acataaagtt tgatggctaa atgataattt
     1021 tggtggacta gagggttatt tctcacttct tctgtcttct ttctaacatt gaattagtat
     1081 tataatgttt tattatttta atattataat taatttcaaa catattttgt ttttctatta
     1141 taacttttaa aatataatgt tatataaaat tatgaatacg tagttatgac gttcttattt
     1201 ttatctacat ttcggtataa gttagtcaaa tataatattt tttaattttt tgttcatgat
     1261 gtaataattt ttctaaattc gaatataccg ttacgatatg tttattttca cctacgtttc
     1321 aatataaatc tatttaaaat aaaccgctct caatctgggg ctaaaaccta tgggtcaagt
     1381 acaatgtatt tttttacaag tattataaaa ttttattatt ttaatattat aactaatttc
     1441 aaacattttt ttttctctta taacttttaa aacataatgt tttgtaaaat tacgaatatg
     1501 tagttgtgac ttgcttattt tatttacatt tcagtataaa ttttgtatga aaccaacccg
     1561 ggtcaaatat aatatatttt taaatttttt tacggcgtag taattttcta aaatttcaaa
     1621 tatgtcatta cgatatgctt attttcacct attttttaat ataaatttta tttaaaatcg
     1681 atcactcgca atgtgcggct aaaatctatg ggtcaagtat aatgtatttt ttatttattt
     1741 tttacaccaa cgtaaatttt tctaaatttt ccccaacaac gtgatgttct cctaaatttg
     1801 aatataccat tatgacatgg ttatttccac caacgtttta ataaaaaaat tatttaaaac
     1861 cagccccaca atgcgtggcc gaaactcacg gatcaaatat agctttcttc acactaacgt
     1921 ttttatgttt cgactatacc gttgtgacgt actgttttct acctacgttt ctaaataaat
     1981 tatatttaaa aacatgccgc tgcaacacgc gagaaaacta ctagttgttt tctaattcac
     2041 aaaaattctc aacgaatgat tagttgcgtt tgttatgcaa acgaaagcgg acgatcatgt
     2101 cgttattatt aattaaaaaa aatactaaaa gagtaaaaaa tagaagaaaa agactgatta
     2161 tcaatttaat aataatatcc acaaaaatat tccaataatt taacctgagt ttgctatttg
     2221 tggatgagtt tctgtatcgt tgatacctgt aactcacaca gttcttaact catacttcat
     2281 ctataaatac tcaatcccca attcgttttg tttagagtct caacactgag catacccata
     2341 tctcaatttc atcatcttct tccactattc aggtgatctg attgaacatt tacataacta
     2401 tttgcttaat ttatgttggg tttacagtat ctattggatg gatttcttgt accgttatat
     2461 ggtttgtggt tcgattatgg gtctcgatca gaagtttgtg ataatctggt gaaatgggtt
     2521 gtttgtggtt gtttttgtgt taatttttca gggttggagg ggaattaagc aagtgtgtgt
     2581 gtaatggcta accggaattt ggagaaatta gcatcgatcg atgctcagtt gaggctttta
     2641 gtccctggga aagtttctga ggatgataag cttattgagt atgatgcttt gcttttggat
     2701 aagtttctgg atattcttca agatttgcat ggggaagatc tcaaggaagc ggtatgataa
     2761 ctcaaatcat tgtttgtttt tagcttatac tctgganctt gatgttcctt ttgcttaatc
     2821 cgttacataa gttagatcta aagtgagttg agatatgaat atcactgtta attagatttg
     2881 ggttttatgt gttttataga attggtgaat gtgaatcttc agtttattat cttatatggt
     2941 tttgctggac tacttgtttt gattgatcac caactgtttg acaaaatgcc taaaagggaa
     3001 tattcaatta tttcttagtt tttaccagct cagcctctca tgaaatctag tgctatataa
     3061 gttagtgact accaaagggc ttgtagccta gcggtatcaa gatgtgtact acaggtgttt
     3121 ctccttaagt gattgagaat tttagtctca tagttgacat tggtgtatat ttacgagtat
     3181 gtttagatgg tgtgtgagtt agctgttgta aaatatataa atacgtgcac acagatatat
     3241 aagttggtaa gtatgtttgc ttatgtaggt tcaagaatgc tatgagctat ctgctgaata
     3301 tgaaggaaaa catgacccga agaagctgga ggagcttgga agtgtgttga caagtttaga
     3361 tccaggggat tccattgtca ttgcaaaagc tttttctcac atgcttaact tagccaatct
     3421 ggctgaagaa gttcagattg cttaccgccg aagaatcaaa ctgaagagag gtgattttgc
     3481 tgatgaggct aatgcaacaa ctgaatcaga tattgaagaa actttcaaga aacttgtgct
     3541 taagcttaac aagtcccctg aagaggtttt tgatgcactc aagaatcaaa ctgttgactt
     3601 ggtcttgact gctcatccaa ctcaatccgt ccgcagatct ttgcttcaaa agcatggaag
     3661 gtatagcctt caatcctgta tacgcatcca atttgactga ttaatcaact tcaaaattga
     3721 ccctttatct tttcaggatt cgcaactgtc tggcccagtt gtatgccaaa gacatcactc
     3781 ctgatgataa gcaggaatta gatgaggctt tgcatagaga agtaagtatt agcgattaga
     3841 aatgcattaa gattaaagat gcattgtgtg tgcaagatca ttgttattaa ttgcttttat
     3901 gtaatttgta gattcaagct gcatttcgta ctgatgaaat caggaggacc ccaccaacac
     3961 cacaagatga aatgagagca ggaatgagtt acttccatga aacaatctgg aagggtgttc
     4021 ctaaattctt acgtcgtgtt gacaccgccc taaagaatat agggattaat gaacgtgttc
     4081 cctataatgc acctctaatt caattctctt catggatggg tggtgaccgt gatggcaagt
     4141 ttaagctatt ttatacactt aattgacaac aaaactaaga tttcattcat cacttatata
     4201 attnngcttc tggattaacc tgcaggcatc cgagggtaac tcctgaggta acgagggatg
     4261 tttgtttgct agccagaatg atggcttcaa acatgtactt ttctcagata gaggatctta
     4321 tgtttgaggt atgtgaaaaa aactttctag gatatatgta ctcttgcata gtgattattg
     4381 atcttaacct atgttttctt tttcagatgt ccatgtggcg ttgtaatagt gaactacgtg
     4441 ttcgagcaga agaactatat agaacagcaa gaagagatgt gaagcactac ataggtattt
     4501 gttagtgtta atgatgttat gtattactac atatcaaaac acagaataac gttatatgtt
     4561 tgtctttttc tttagagttc tggaaacagg ttcctcccac tgaaccttat cgtgtaattc
     4621 ttggtgatgt aagggacaaa ttatataata cacgtgaacg atctcgccat ttattagccc
     4681 atgggatatc tgacatccca gaagaagctg tttataccaa tgttgaacag gtatcaaccc
     4741 cttcaagatc accaattatc aatttttgat aaatcctaga ttgcattctt gacctggtta
     4801 tgatcgggtc aatctgggct atcttctatc tctagtgggt caaactagta aagtaaaaaa
     4861 gttagctata aaggaaacgg gtcgaaagtt gccctagatt ttttcttgga taactatgca
     4921 taaaacctct tgattcttat tataaacagt atattttgat tgaaatgtgt aaaatttgtg
     4981 gaaaaagacc agattccacc agcaccaaaa actacacatt ttgacctgct acctaaacct
     5041 cgaggcctgc gcattttacg catctctaat ccttacctta ataattcgaa cttctccctg
     5101 gaccctaccc ttttatgaac aggtcaactt gggctatgtt ttatcgctaa ttgggttaaa
     5161 taaaatagaa tagctaaaac aaaaatgtgg tcaaatgggt tgaaaatcgc ccaaaatgta
     5221 ttcattataa aacctcctga ttaatttatg aaaagtatat agcgattgaa aaagtataat
     5281 ttttgtaatc acatttgtcg taccgataat tttaaaaact gtaaaacttt tggacaacaa
     5341 tcagattcaa cgggcacata aaattaccca ttttgacctg ttaccccaat gccttgcctc
     5401 taatgaaatt ttttctttag gctctctcta atatctcttt tataatattt cacagttctt
     5461 ggaaccactg gagctatgct acagatcact atgtgactgt ggtgaccgtg tgattgctga
     5521 tggaagcctt cttgattttc taagacaagt gtcgactttt ggactctcac ttgtaaaact
     5581 tgatataaga caagaatctg accgtcacac tgacgtcctt gatgcaatca ctcaacattt
     5641 agaaattggg tcctaccgtg agtggtctga agaaaaacgc caagaatggc ttctagctga
     5701 actcagtgga aaacgtcctc ttttcggttc agaccttcca aaaactgagg aagttaagga
     5761 tgttttagac acgtttaatg ttttagcaga actcccatct gactgtttcg gtgcttacat
     5821 catctcaatg gccacatcac cttctgatgt ccttgctgtt gagcttctcc aacgtgaatg
     5881 ccatgtaaaa catccgttac gcgtggtccc cctatttgaa aaacttgctg acctagaggc
     5941 ggcccctgcg gccatggccc gccttttctc aatcgattgg tacagaaatc ggatcgacgg
     6001 taaacaagaa gtcatgattg ggtactctga ttcaggaaaa gatgcaggcc ggttttctgc
     6061 tgcatggcag ctctacaaag ctcaagaaga gattattaaa gttgcaaaag agtttggggt
     6121 caaacttgtt atatttcatg ggcgtggggg gactgttggt agaggtggcg ggcccacaca
     6181 tttagctatc ctctctcaac caccagacac cattcacggg tcgttaagag tcacggttca
     6241 gggtgaggtc atagagcagt cgtttggtga ggaacatttg tgttttagaa cacttcagag
     6301 attttgtgca gctacacttg agcatgggat gaacccacca atttcaccac ggcctgagtg
     6361 gcgtgaactt atggaccaga tggctgttgt tgcaaccgag gagtaccgtt ctattgtgtt
     6421 taaggaacca cgttttgtgg agtatttccg ccttgtaagt atacttataa ttgcttatga
     6481 ttatagttgg atgtggcaaa atggattggt tgggtctcat atcaagttgg gttcgggtca
     6541 aaatggtaat atttggtaca tttcggccag gaaacacatt gtagccacaa ataaataact
     6601 atcagttttg ataaacatag ccttgtgacc atgtttaaga actaaggaca tgaataaatt
     6661 actgcacttt aaacataacc accgcaaggt ggttgataat tagtgattaa tgtataatga
     6721 tttagcatgt ccttagttcc tatacataat ccttatggct atatttatca aaaccctata
     6781 agtacgtaac acatgatttc caaaatatat tcattgataa tctagtgttt tgaactgtag
     6841 cagttactta taagactttg tttcactaaa tagacantag gaaagcaagg ttgtggttgc
     6901 ggattatcac tttgtggttt tattttattt actatggggt gggtttatat ggatttttct
     6961 ttgtgtcttt tgaaagggta aggctgcacc caacccataa ctgacctatc tatacacctg
     7021 caggcaacac ctgaattgga gtacgggcgt atgaatattg gaagtcgccc atcaaaaaga
     7081 aaacctagtg gtggcattga atcactcaga gccattccat ggatctttgc atggactcag
     7141 accaggttcc atctcccagt ttggcttggg tttggagcgg cattcaaaca tgccattaaa
     7201 aaagacagca agaatcttca aatgcttcaa gaaatgtaca aaacatggcc tttctttcgg
     7261 gtcaccattg atttagttga aatggtgttt gctaaaggag acccaggcat tgctgccttg
     7321 aatgacaaac tccttgtttc tgaagatcta tggccttttg gagaatcttt gagagcaaac
     7381 tatgaagaaa ccaaagatta tcttctcaag gtacaatttc tcaacattta taactcatgc
     7441 ttttggatgg gtcaggcagg ttggataaca ggtcaatgta ggtttaattg agacgaatca
     7501 tttctcaaca ctccgtcttt ctattataaa aatgaagggt ttttgcataa aagtcaaagg
     7561 tacttgttta tctgtcatat tttagtcatt gtaatgnntt tgtgtacggt tcaggtcatt
     7621 ttgcaagtcg gtaaatgtac aaaatgacct aaacgggaca caaagtcatc ttcaattact
     7681 catatgcatg gaaagaagta caatgactaa aaccgacaaa tattaattaa attgcttttt
     7741 ttgtgnaaaa aaattaataa taaatgcatt tttttatgaa ttcatgatat aaaaaataat
     7801 aatagtacta gaataattta agtcctatta tacaacaatc taggttgtca tacacataaa
     7861 aaagagacca tttctcgagt tttaatctat ttgaccttcc atttagctta gttctttttt
     7921 tctttgttcc atttgatccg tttttcggtt tgatccattt gagataacac gcaacccaaa
     7981 tcaacccgtt aataagtaaa taggttgaaa ctgccacctc taaattatta tgctaaccgg
     8041 attctcttgt cgggttttag attgctggac acagggacct tctagagggt gatccctact
     8101 taaaacaaag aatcaggctg cgtgattcat acatcacaac cttaaatgta tgtcaagctt
     8161 ataccctaaa gcggatccgc gacccgaact atcatgtgac attaaggcct catatttcca
     8221 aagaatacgc cgccgagccg agcaaaccag ctgacgagct tatccacctg aacccaacca
     8281 gtgaatacgc acccggtttg gaggacacgc tcatcttgac catgaaaggg attgctgctg
     8341 gaatgcagaa caccggttag attaaccgtt atgcaagtgt gtagttatct tttcatgctt
     8401 ttggatattg tcacatgcct tgtttactgc atgtgttctt gagtgtttgt attcatcatc
     8461 aatcaatgca ttgcagaagt tgtactatgt gcatcattga tggtggttta tccaagaaac
     8521 taatgactta ttgtgcactt gaatacaagt gattatataa attgttgtgc tttctactta
     8581 ttctaccctc ttagcagatt cattctatag aaccatttac caaatcgact ctatgattaa
     8641 gattgaaacg gtgattaaat tgtaactaat aggaagttaa atatgtctag gaggacaaaa
     8701 gaggaaatac agttataaag ttttactttc tctataaaat agttttcact tatattttta
     8761 atctctaata ttttcaaata tacttttatt atttttttaa gaattatgat acggaatcaa
     8821 aaaatcatga aaataacttt tctaga
//