From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Bradyrhizobium japonicum heme-copper oxidase subunit I homolog
Date: 29 Dec 92 00:47:00 GMT
Distribution: bionet
Checksum: 25799 13

LOCUS       RHMFIX       5212 bp ds-DNA             BCT       03-DEC-1992
DEFINITION  Bradyrhizobium japonicum heme-copper oxidase subunit I homolog
            (fixN), cytochrome c (fixO), transmembrane proteins (fixO and fixQ)
            diheme cytochrome c (fixP) and fixG genes, complete cds.
ACCESSION   L07487
KEYWORDS    cytochrome c; diheme-cytochrome c; fixG gene; fixN gene; fixO gene;
            fixP gene; fixQ gene; heme-copper oxidase;
            membrane-anchored protein.
SOURCE      Rhizobium japonicum (library: USDA 3I1b11spc4) DNA.
  ORGANISM  Rhizobium japonicum
            Prokaryotae; Gracilicutes; Scotobacteria; Rhizobiaceae.
REFERENCE   1  (bases 1 to 5212)
  AUTHORS   Preisig,O., Anthamatten,D. and Hennecke,H.
  TITLE     Genes for a novel, microaerobically induced oxidase complex in
            Bradyrhizobium japonicum are essential for a nitrogen-fixing
            endosymbiosis
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     CDS             9..842
                     /function="?"
                     /gene="ORF277"
                     /citation=[1]
                     /note="DNA-region 1 to 268 is overlapping with
                     fixLJ-region (EMBLAccession no. X56808).; putative"
                     /product="."
                     /codon_start=1
                     /translation="MTYATVMVSLALDQSNEARLQVAGELAERFEAAIVGVAAAQFAP
                     PLYFTDGAAAQGLIDEGEASVRRRLAALEAQFRAATRNRGGHAEWRSAMDFPARFALA
                     QARCADIVVSGGQSPAFSDAFALASPKDLVMQAGRPLLVVPDGANWLDLRSVLVAWKD
                     TPEARRAVADALPMLRKARDVTIAAIPERDDDRSVVMAGVTDVAAWLARHGVTATARV
                     SEAAGDEPAAAQLEQVAGDVGAGLIVAGAYGHSRFRELILGGVTQYLVTQSARSVLLS
                     H"
     CDS             881..1306
                     /function="unkown"
                     /gene="ORF141"
                     /citation=[1]
                     /note="putative"
                     /product="."
                     /codon_start=1
                     /translation="MYRFLEQTVDGYMTRNVKTVQRDIDMLELSEMFERDDFNSYPVE
                     DDGQVVGIVTKFDILKCFAFTPSQMLPRYHDLMSRKIGDVMTPEFIYVSPDTRLTRVL
                     QIMVEHRIRSIIVLDGAQKLVGIIAREDVIAALKATARD"
     CDS             1516..3165
                     /function="homolog to subunit I of heme-copper oxidases"
                     /gene="fixN"
                     /evidence="EXPERIMENTAL"
                     /citation=[1]
                     /product="membrane protein"
                     /codon_start=1
                     /translation="MSQPSISKSMTIGESGLAVVFAATAFLCVIAAAKALDAPFAFHA
                     ALSAAASVAAVFCIVNRYFERPAALPPAEINGRPNYNMGPIKFSSFMAMFWGIAGFLV
                     GLIIASQLAWPALNFDLPWISFGRLRPLHTSAVIFAFGGNVLIATSFYVVQKSCRVRL
                     AGDLAPWFVVVGYNFFILVAGTGYLLGVTQSKEYAEPEWYADLWLTIVWVVYLLVFLA
                     TIIKRKEPHIFVANWFYLAFIVTIAVLHLGNNPALPVSAFGSKSYVAWGGIQDAMFQW
                     WYGHNAVGFFLTAGFLAIMYYFIPKRAERPIYSYRLSIIHFWALIFLYIWAGPHHLHY
                     TALPDWTQTLGMTFSIMLWMPSWGGMINGLMTLSGAWDKLRTDPVLRMLVVSVAFYGM
                     STFEGPMMSIKVVNSLSHYTDWTIGHVHSGALGWVGFVSFGALYCLVPWAWNRKGLYS
                     LKLVNWHFWVATLGIVLYISAMWVSGILQGLMWRAYTSLGFLEYSFIETVEAMHPFYI
                     IRAAGGGLFLIGALIMAYNLWMTVRVGEAEVQMPVALQPAE"
     CDS             3178..3912
                     /function="contains a heme binding site, putative
                     cytochrome c"
                     /gene="fixO"
                     /evidence="EXPERIMENTAL"
                     /citation=[1]
                     /note="fixO gene encoding protein with a putative
                     transmembrane helix"
                     /product="transmembrane protein"
                     /codon_start=1
                     /translation="MSFWTRHQVFEKNSIILIVGILLVIAIGGLVEITPLFYLKSTIE
                     KVDGVRPYTPLELAGRNVYVREGCYLCHSQMIRPLRDEVERYGHFSLAAESMFDHPFQ
                     WGSKRTGPDLARVGAKYSDDWHVTHLTNPRAIVPQSVMPGYPFLSATEVDPDTIADHM
                     RTLRTVGVPYTDDQIANASADLKAQADPDNAGADAFNKRYAKAVVRNFDGKTGTPTEM
                     DALIAYLQMLGTLVDFKIYNEKANLR"
     CDS             3926..4090
                     /gene="fixQ"
                     /citation=[1]
                     /note="fixQ gene encoding protein with a putative
                     transmembrane helix; putative"
                     /product="transmembrane protein"
                     /codon_start=1
                     /translation="MKAILTLDNLASGLVTTIWTPVFVAIFLAIIAYAFWPRNKAAFD
                     EAAHLPLREE"
     CDS             4097..4969
                     /function="diheme-cytochrome c"
                     /gene="fixP"
                     /evidence="EXPERIMENTAL"
                     /citation=[1]
                     /product="membrane-anchored protein"
                     /codon_start=1
                     /translation="MTDHSEFDSVSGKTTTGHEWDGIKELNTPLPRWWVICFYLTIVW
                     AIGYWIVYPAWPLISSNTTGLFGYSSRADVAVELANLEKIRGDKMAALGAASLADVEK
                     DPALLALARAKGKTVFGDNCAPCHGSGGAGAKGFPNLNDDDWLWGGTLDQIMQTIQFG
                     ARSGHAKTHEGQMLAFGKDGVLKGDEIVTVANYVRSLSGLPTRKGYDAAKGEKIFVEN
                     CVACHGDGGKGNQEMGAPNLTDKIWLYGSDEAALIETISQGRAGVMPAWEGRLDPSTI
                     KAMAVYVHSLGGGK"
     CDS             5081..5212
                     /function="homolog to FixG from Rhizobium meliloti"
                     /gene="fixG"
                     /citation=[1]
                     /partial
                     /note="putative"
                     /product="FixG"
                     /codon_start=1
                     /translation="MNKTVNPKDLTSEDDVGPLYAARKKVYPQSVSGTFRRIKWGLMA
                     "
BASE COUNT      906 a   1737 c   1600 g    969 t
ORIGIN      
        1 gaattctaat gacatacgcg accgtgatgg tcagcttggc gctcgaccag tccaacgagg
       61 cgcgtcttca ggtcgcgggc gagctcgccg agcgattcga ggccgccatc gtcggggttg
      121 ccgcggcgca gttcgccccg ccgctctatt tcaccgacgg cgccgccgcc cagggactga
      181 tcgacgaggg cgaagcgtcc gtcaggcgac ggctggcggc tctggaggcg caattccgcg
      241 cggccaccag gaatcgcggc gggcatgccg agtggcgcag cgccatggat tttccggcgc
      301 gattcgccct ggcgcaggcg cgctgcgccg acatcgtcgt cagcggcggg cagagcccgg
      361 ccttctccga cgctttcgcg ctcgcaagcc ccaaggatct ggtgatgcag gccggccgcc
      421 cgcttctcgt cgtccccgat ggggccaact ggctcgacct gcgcagcgtg ctggtggcct
      481 ggaaggatac gccggaggca cggcgggcgg tggcggacgc gctgccgatg ctgcgcaagg
      541 cgagggacgt caccatcgct gcaattccgg agcgcgacga cgaccgctcg gttgtgatgg
      601 ccggcgtcac cgacgttgcc gcctggctcg cccgtcacgg cgtcaccgcg accgcgcgcg
      661 tctcggaggc tgcgggagac gaacccgccg ccgcgcaatt ggagcaggtc gccggcgacg
      721 tcggagccgg cctgatcgtt gcgggcgcct atggacattc gcggtttcgc gagctgatcc
      781 tgggaggcgt cacccagtat ctggtcactc aatccgcccg tagcgtgttg ctgtcgcact
      841 gacggccgtt cggccaaaag ctattcgagg aggacgcgcc gtgtacagat ttcttgagca
      901 gaccgtcgac ggctacatga cgcgcaacgt caagacggtg caacgtgaca tcgacatgct
      961 cgagctcagc gagatgttcg agcgcgacga tttcaactcc tatccggtcg aggacgacgg
     1021 acaggtggtc ggcatcgtca ccaagttcga catcctgaaa tgcttcgcct tcacgccgag
     1081 ccagatgctg ccgcgctatc atgacctgat gagccgcaag atcggcgacg tcatgacgcc
     1141 ggagttcatc tatgtcagcc ccgacacgcg gctgacgcgc gtgctccaga tcatggtcga
     1201 gcaccgcatc aggagcatca tcgtgctcga cggcgcgcag aagctcgtcg gcatcatcgc
     1261 ccgcgaggac gtcatcgcgg cgctcaaggc gaccgcgcgc gactgacggg gtctccagtt
     1321 cccgcgattt caaactccgt gatttcacgg aagcgatcgg tcgccgcgcg cgcggcgccg
     1381 gccgcaggcg attgatatgg ctctctgatc cgcgaaagcc tcacattaac agaaatccac
     1441 ctatcttgat ttcaatcaat tccccgcgag ggtgaatgtg gtttctggcg ctgtgttctt
     1501 tcagagagaa tccgcatgag ccagccctcc atctccaaat ccatgaccat cggtgaaagc
     1561 ggcctggctg tcgtgttcgc agccaccgcc ttcctctgcg tgatcgccgc ggccaaggcg
     1621 ctcgacgcgc ccttcgcctt ccacgccgcg ctcagcgcgg cggcgagcgt ggcggcggtg
     1681 ttctgcatcg tcaaccgcta cttcgagcgc ccggcggcgc tgccgccggc ggagatcaac
     1741 ggccgtccca actacaacat ggggccgatc aagttctcct ccttcatggc gatgttctgg
     1801 ggcattgccg gcttcctcgt cggcctcatc atcgcctcgc agctggcctg gcctgcgctg
     1861 aacttcgatc tgccctggat cagtttcggc cgcctgcgtc cgctgcacac ctctgccgtg
     1921 atcttcgcct tcggcggcaa cgtgctgatc gcgacatcct tctacgtcgt gcagaagtcc
     1981 tgccgcgtgc gccttgccgg cgatctcgcg ccctggttcg tcgtggtcgg ctacaacttc
     2041 ttcatcctgg tcgcgggcac cggctatctg ctcggcgtca cccagtcgaa ggaatatgcc
     2101 gagccggaat ggtacgccga tctctggctg accatcgtct gggtggtcta tctgctcgtc
     2161 ttcctcgcca ccatcatcaa gcgcaaggaa ccgcacatct tcgtcgcgaa ctggttctat
     2221 ctggccttca tcgtgacgat cgcggtgctg catctcggca acaaccccgc gcttcccgtc
     2281 tcggcgttcg gctcgaagtc ctacgtcgcc tggggcggca tccaggatgc gatgttccag
     2341 tggtggtacg gccataacgc ggtcggcttc ttcctgaccg ccggcttcct cgccatcatg
     2401 tactacttca tcccgaagcg ggccgagcgg ccgatctatt cctaccgcct gtcgatcatc
     2461 cacttctggg cgctgatctt cctctacatc tgggcaggtc cgcaccatct gcactacacg
     2521 gcgctgcccg actggacgca gacgctcggc atgaccttct cgatcatgct gtggatgccc
     2581 tcctggggcg gcatgatcaa cggcctgatg acgctgtcgg gcgcctggga caagctgcgc
     2641 accgaccccg tgctgcgcat gctcgtggtc tccgtcgcct tctacggcat gtcgaccttc
     2701 gaaggcccga tgatgtcgat caaggtggtc aactcgctca gccactacac cgactggacc
     2761 atcggccacg tccactccgg cgcgctgggc tgggtcggct tcgtctcctt cggcgcgctg
     2821 tattgcctgg tgccgtgggc ctggaatcgc aagggcctct acagcctgaa gctcgtcaac
     2881 tggcacttct gggtcgcgac cctcggcatc gttctctaca tctcggcgat gtgggtgtcc
     2941 ggcatcctcc agggcctgat gtggcgcgcc tacacctcgc tcggcttcct cgaatattcc
     3001 ttcatcgaga ccgtcgaggc gatgcatccc ttctacatca tccgtgctgc cggcggcggg
     3061 ctgttcctga tcggcgcgct gatcatggcc tacaatctct ggatgaccgt tcgcgtcggc
     3121 gaggcggaag tccagatgcc cgtcgctctt cagccggcgg aatgaggagc taaagcaatg
     3181 tcgttctgga cacgccacca agtcttcgaa aagaactcga tcatcctgat cgtcggcatc
     3241 ctcttggtga tcgcgatcgg cggcctcgtc gagatcactc cgttgttcta cctcaagagc
     3301 acgatcgaga aggtcgatgg agtgaggccc tatacgccgc tggagctcgc cggccgcaac
     3361 gtctacgtcc gcgaaggctg ctatctctgc cattcgcaga tgatccggcc gctgcgcgac
     3421 gaagtcgagc gctacggcca cttctcgctg gccgccgaga gcatgttcga ccatccgttc
     3481 cagtggggct ccaagcgcac cggtccggac ctcgcccgcg tcggcgccaa gtactccgac
     3541 gactggcacg tcacccatct gaccaacccg cgcgcgatcg tgccgcagtc ggtgatgccg
     3601 ggatatccgt tcctcagcgc aaccgaggtc gatccggaca ccatcgccga ccatatgcgt
     3661 acgctgagga ccgtcggcgt gccctacacg gacgaccaga tcgccaacgc gagcgccgat
     3721 ctgaaggccc aggccgatcc ggacaatgcc ggcgccgacg ccttcaacaa gcgctacgcc
     3781 aaggccgtgg tgcgcaactt cgacggcaag accggcacgc cgaccgagat ggacgcgctg
     3841 atcgcgtatc tgcagatgct cggcacgctg gtcgacttca agatctacaa cgagaaagcc
     3901 aatcttcgct gagaaggcat caacgatgaa agccatcctg accctcgaca atctcgcgtc
     3961 cggtctcgtg accacgatct ggacgccggt gttcgtcgcg atctttctcg cgatcatcgc
     4021 ctacgcattt tggccccgta acaaggctgc gttcgacgaa gcagcacacc tgccgttgcg
     4081 ggaggagtga gagaccatga ccgatcatag cgaattcgat tctgtctccg gcaagacgac
     4141 gaccggacat gagtgggacg gcatcaagga gctcaacacg ccgctgccgc gctggtgggt
     4201 gatctgcttc tacctcacca tcgtctgggc gatcggctac tggatcgtgt atccggcctg
     4261 gccgctgatc tccagcaaca ccacgggctt gttcggctac tcctcgcgcg ccgacgtcgc
     4321 ggtcgagctc gccaatctcg agaagatcag gggcgacaag atggcggcgc tcggcgcggc
     4381 ctcgctcgcc gacgtcgaga aggacccggc cctgctggcg cttgcgcgcg ccaagggcaa
     4441 gaccgtgttc ggcgacaatt gcgcgccgtg ccacggctcc ggcggcgccg gcgccaaggg
     4501 cttcccgaac ctgaacgacg acgactggct gtggggcggc acgctcgacc agatcatgca
     4561 gaccatccag ttcggcgcgc gttccggcca cgccaagacg catgagggcc agatgctcgc
     4621 cttcggcaag gacggcgtgc tgaagggtga cgagatcgtc acggtcgcca attacgtgcg
     4681 gtcgctgtcg gggctgccga cccgcaaggg ttacgacgcc gccaagggcg agaagatctt
     4741 cgtcgagaat tgcgtcgcct gccacggcga tggcggcaag ggcaaccagg aaatgggcgc
     4801 accgaacctg accgacaaga tctggctcta tggctcggac gaggctgccc tgatcgaaac
     4861 catcagccag ggccgcgccg gcgtcatgcc ggcctgggaa gggcggctgg atccctccac
     4921 catcaaggcg atggcggtct acgtccactc gctgggcggt ggaaaatagc agatccgcgg
     4981 caaacggggg cgaacaaaag tgcccccgtt tgagctggat caacggacgc ggcgacgtcg
     5041 ggtctaggtt taatcgcacc tcctcgaaaa gtttcaggcg atgaacaaga ccgtgaaccc
     5101 gaaagacctc acatcggaag atgacgtcgg gccgctctac gcagcccgca agaaggtcta
     5161 tccccagagc gtctccggca cgttccgccg gatcaaatgg ggcctgatgg cg
//