From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Plasmid pTiS4 (from Agrobacterium tumefaciens) T3 isopentenyl (ipt)
Date: 29 Dec 92 19:49:43 GMT
Distribution: bionet
Checksum: 37626 6

LOCUS       IS4IPTVT     3271 bp ds-DNA             BCT       09-DEC-1992
DEFINITION  Plasmid pTiS4 (from Agrobacterium tumefaciens) T3 isopentenyl (ipt)
            and psuedo vitopine synthase genes, complete cds.
ACCESSION   M91610
KEYWORDS    isopentenyl transferase; vitopine synthase.
SOURCE      Plasmid pTiS4 DNA.
  ORGANISM  Plasmid pTiS4
            Prokaryotae.
REFERENCE   1  (bases 1 to 3271)
  AUTHORS   Canaday,J., Gerard,J.-C., Crouzet,P. and Otten,L.
  TITLE     Organization and functional analysis of three T-DNAs from the
            vitopine Ti Plasmid pTiS4
  JOURNAL   Mol. Gen. Genet. 235, 292-303 (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     TATA_signal     588..593
                     /gene="ipt"
     misc_signal     complement(1337..1344)
                     /note="Chi sequence"
     polyA_signal    1450..1455
     CDS             complement(1883..2816)
                     /pseudo
                     /product="vitopine synthase"
                     /codon_start=1
     polyA_signal    complement(1948..1952)
     CDS             656..1366
                     /function="shoot formation; cytokinin synthesis"
                     /gene="ipt"
                     /note="ORF 6b"
                     /product="isopentenyl transferase"
                     /codon_start=1
                     /translation="MEAHLIFGPTSTGKTSVAIALAKRTGFPVIVLDRIQCYSQLSVG
                     GGRPSAAEFQGTRRIYLIEGSLDEGVISAERAHECLVAAVEAHKPEGGVILEGGSISL
                     FKRMAQSSYWNCGFTWHVTRLHLGGEEIFLAAAKKRINQMMQPDEQGNSFLGELVSVW
                     KTTALRATLEGICGYRYAIEFAGKQGLEMDALTSLNRRQLEQLVHGMAHEYLCYARQQ
                     EQELPLPSLAGGEGPPFQ"
BASE COUNT      841 a    737 c    806 g    887 t
ORIGIN      2.8 kb right of T-DNA T2
        1 aagcttttcc tggctcgccg tcgtggtgac tgcgtatccg acgaacagct tcgccgtttt
       61 ccaactggtt catcgttgag ccagaaccat cattgctcgc ctcctatatg atcgagtggc
      121 tgatggcagg atatatcaaa gtgtaagtgt caatcgagtg cttcccacct gccgaaacat
      181 caaatgcctc gcctttgtct gtaggtgatt gcaaaacgac agaatgattg accccgcttg
      241 ctggcgctct ggagcaatta gtcaatattc cttcttagtg cggggcggct gtatgcgaag
      301 ggtgggccac ctccgaagac ttaattggaa aaagtttgtc gttgtaggga agacgtaagc
      361 acttccgtca tcgcttcact attgttgctt cttacagatc gtcgctgtac agactataaa
      421 ggtcagcagt tcattgtctg ggccacttgc tcagttcacc tttgagattt caacaaatgc
      481 ggcgcgcctt accaaaagtc gaatggtgtc cccaccagtt gttgagagag ctaatcgatt
      541 tgaccgtcaa tcagttatac tgaatgttac catatgagat atcgctctat aaattggcca
      601 ttttgcaaat ccaaacccaa gcgctcaaaa ctacaagata ttgagcagac aaaatatgga
      661 agcacacttg atcttcgggc cgacatctac gggaaaaact tcagttgcaa tagctcttgc
      721 taaaagaact ggttttcctg tgatagtact ggaccgaatc caatgctact cccagctttc
      781 tgttggaggt gggcgacctt ctgctgcaga atttcaagga accaggcgta tttacctgat
      841 tgaaggatcg cttgatgaag gtgtaatcag cgctgaacga gctcatgaat gtctcgtcgc
      901 agcagtcgaa gctcacaagc ccgagggtgg ggtgatactt gaggggggct caatctcact
      961 gttcaaacgt atggctcaaa gcagctattg gaattgtggc tttacatggc atgtcacccg
     1021 acttcacctc ggcggtgaag agatttttct ggcagcggcg aagaagagga tcaatcagat
     1081 gatgcaaccg gatgagcagg gaaattcatt tttgggagaa ctggtctctg tctggaagac
     1141 aactgcgctg agagccacac ttgagggaat atgtggctat cgttatgcta tagaatttgc
     1201 tgggaagcag ggtctggaga tggatgcact tacaagcctg aaccgtcgtc aacttgagca
     1261 gttggtgcac ggtatggcgc atgaatatct ctgctacgcc cgtcaacagg aacaagaact
     1321 tcccttgccc tcgcttgctg gtggggaggg acctccattt cagtagcagt aggcttgcct
     1381 atctgcgcaa ttctccaaaa taccgttgct ccctacttct aaggcgtaca atactactgt
     1441 tatatagcta ataaaattgt acgcagtgtg tttattatga tgcgactgac ggggactttg
     1501 ttgctaagtt tcccggtata agggcgacat tctattgtcg ttaatgtaat atgtatgttt
     1561 gaataaacgc ttttatgttc ctatttgcgt ttttggtgtt tattcgcaat gttaaatatc
     1621 ggaattgtcg atatagtttg ttattatatt gtgttttcgt tttgtttgta gattatttgt
     1681 ttaaaactaa atttatttgt gaatctataa taagaaaatg cgtaatattt cacaatataa
     1741 tatattatac agtagtgctg gccaattgta tcgaacgtgc cagacattat tcaaacctct
     1801 cgcaaaacag aaaattaaaa cacaccctgg atgcttctat gcatcacaat atattacatc
     1861 agatcaagct gagagacgga tttcagtttc cattgagtgc ctcaacgatg cgattcaggg
     1921 gcgcgcgagc taaccccagc gaagcaagag ttcggccagt ttcaaagagg ttctcctcga
     1981 gaacaaggcc ggcctcctcg ataacagagt tcattgctgg ggcctgccct ccataccatt
     2041 ttttcgagta ttcgaaatct gtttcagagt gttgaccgag cgcgcgaaca atctcaaggc
     2101 gttcattgtc taatgcgaga acacgctcga tcgcttgcgg cacaaacccc cggtagaatt
     2161 tcgggagagg gctcagcccg cattcatcgc gactcttgcc gctagtatgc caggaggatg
     2221 tgcaaccggg ttggtattcg ataaaaatat ggaggcagta tttttgtacc actgcagtcg
     2281 gtttgggaaa agcgcctcaa agcgacttgt gagatcctcg ctgagaggcc gtgttgccgc
     2341 aacctcgaaa caggttttca cgccaagcat attaacgcgt ttacccacac ggcggcaggc
     2401 atatggggat gtggttgact caatgattgc agcaggcgac aaactggggg ataggatttt
     2461 cttgcaagtc aaagacgttg cactgcccgg caaagctacg agcacagagt gactcaggtc
     2521 ggattcagca actgccgtag aataccctca tgtcccaacg ttggaactgt cagaaagacg
     2581 aactctgcgt ccgagattat ggcttggaga tcatcctcca actgcggctc gaagctgcct
     2641 ccataggtcg ggcctaccag ttgcagcgtg actatgcttc tgacttgagt gtaattgctt
     2701 cggttggaag gtggtgccca gataacggca tggactcgac cgctgagcct cagagcgata
     2761 tcgccggcaa gggtgagagc aaggttccct gcgccgagaa tagctatctt ggtcattgta
     2821 aaattggaag gattttgtgg tatggacccc cggtaagttg gccattaaat agaaggatcg
     2881 gggcgggaaa gtccaatttg ccggcccaac tttcgcgact tagcggtaaa atagatcact
     2941 gcatacgtca gacgcatgac tttattggcc taaatcatct ttatgtgggg tcgggagaag
     3001 cagaaaaagc agcacattca aatgaccaat tattgcaagc agaattcgtg gttcacgtaa
     3061 gccctgacgt cttttgttgg caggatttat tgctaagtca tctttattag tttgtgcatt
     3121 ggtaaattcg ttcataattt caagactcta taaccgatgt tctgccccaa cgtcgtcctc
     3181 cccatccaaa tctgtgatcg cttttccaaa ggacgcgcaa caggagtttc tccatggaga
     3241 gtccattgga gattctcacc cctaggaagc c
//