From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Escherichia coli flagellar protein (fliD) gene, complete cds;
Date: 5 Nov 92 23:05:59 GMT
Distribution: bionet
Checksum: 25300 7

LOCUS       ECOFLGPRO    2400 bp ds-DNA             BCT       27-MAY-1992
DEFINITION  Escherichia coli flagellar protein (fliD) gene, complete cds;
            flagellar protein (fliS) gene, complete cds; flagellar protein
            (fliT) gene, complete cds; ORF1, 5' end.
ACCESSION   M85240
KEYWORDS    flagellar protein; fliD gene; fliS gene; fliT gene.
SOURCE      Escherichia coli (strain JA11) DNA.
  ORGANISM  Escherichia coli
            Prokaryotae; Gram-negative facultatively anaerobic rods;
            Enterobacteriaceae.
REFERENCE   1  (sites)
  AUTHORS   Raha,M., Kawagishi,I., Mueller,V., Kihara,M. and Macnab,R.M.
  TITLE     Escherichia coli produces a cytoplasmic alpha-amylase, AmyA
  JOURNAL   J. Bacteriol. 174, 6644-6652 (1992)
  STANDARD  full automatic
REFERENCE   2  (bases 1 to 2400)
  AUTHORS   Macnab,R.M., Irikura,V.M., Williams,A.W., Mueller,V. and
            Kawagishi,I.
  TITLE     Subdivision of flagellar region III of the Escherichia coli and
            Salmonella typhimurium chromosomes and identification of two
            additional genes
  JOURNAL   J. Gen. Microbiol. 138, 1051-1065 (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     CDS             101..1507
                     /function="filament capping protein"
                     /standard_name="FliD"
                     /evidence="EXPERIMENTAL"
                     /citation=[2]
                     /gene="fliD"
                     /product="flagellar protein"
                     /codon_start=1
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSTTSSTTAFSATTAGNAIAGKYTISVTHL
                     AQAQTLTTRTTTDDTKTAIATSDSKLTIQQGGDKDPITIDISAANSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASSNGMEV
                     SVAAQNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKAQTAIK
                     DWVNAYNSLIDTFSSLTKYTAVDAGADSQSSSNGALLGDSTLRTIQTQLKSMLSNTVS
                     SSSYKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKTGITTTIGS
                     NLTSWLSTTGIIKAATDGVSKTLNKLTKDYNAASDRIDAQVARYKEQFTQLDVLMTSL
                     NSTSSYLTQQFENNSNSK"
     CDS             1532..1942
                     /function="unknown"
                     /standard_name="FliS"
                     /evidence="EXPERIMENTAL"
                     /citation=[2]
                     /gene="fliS"
                     /product="flagellar protein"
                     /codon_start=1
                     /translation="MYAAKGTQAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRASL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSLIQDPV"
     CDS             1942..2307
                     /function="unknown"
                     /standard_name="FliT"
                     /evidence="EXPERIMENTAL"
                     /citation=[2]
                     /gene="fliT"
                     /product="flagellar protein"
                     /codon_start=1
                     /translation="MNHAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVDPSTTMQEQLRPMLRLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     CDS             2385..2400
                     /function="unknown, but not a flagellar protein"
                     /note="ORF1"
                     /evidence="EXPERIMENTAL"
                     /citation=[2]
                     /partial
                     /product="FliT"
                     /codon_start=1
                     /translation="MRNPT"
BASE COUNT      689 a    643 c    580 g    488 t
ORIGIN      
        1 attcccgcta ttaaaaaaaa caattaaacg taaactttgc gcaattcaga ccgataaccc
       61 cggtattcgt tttacgtgtc gaaagataaa aggaaatcgc atggcaagta tttcatcgct
      121 gggagtcggg tcaggtctgg atttaagttc catccttgat agcctcaccg ccgcgcaaaa
      181 agcgacgcta acccccattt caaatcagca atcgtcgttt accgctaaac ttagcgccta
      241 cggtacgctg aaaagcgcgc tgacgacttt ccagaccgcc aatactgcat tgtctaaagc
      301 cgatcttttt tccgccacca gcaccaccag cagcaccacc gcgttcagtg ccaccactgc
      361 gggtaacgcc atcgccggga aatacaccat cagcgtcacc catctggcgc aggcgcaaac
      421 cctgaccacg cgcaccacca cagacgatac gaaaacggcg atcgccacca gcgacagtaa
      481 actcaccatt caacaaggcg gcgacaaaga tccgattacc attgatatca gcgcggctaa
      541 ctcatcgtta agcgggatcc gtgatgccat caacaacgca aaagcaggcg taagcgcaag
      601 catcattaac gtgggtaacg gtgaatatcg tctgtcagtc acatcaaatg acaccggcct
      661 tgataatgcg atgacactct cggtcagcgg tgatgatgcg ctacaaagtt ttatgggcta
      721 tgacgccagt gccagcagca acggtatgga ggtctcggtt gccgcccaga atgcgcagct
      781 gacagtcaac aacgtcgcca tcgagaacag cagcaacacc atcagcgacg cgctggaaaa
      841 catcaccctg aacctgaacg atgtcaccac gggcaaccag acgctaacca tcactcagga
      901 cacctccaaa gcgcaaacgg cgattaaaga ctgggtgaat gcctacaact cgctaataga
      961 taccttcagc agcctgacca aatacaccgc cgtagatgcg ggagctgata gccagagttc
     1021 tagcaatggt gcactgctcg gcgactccac gctgcggacg attcagacgc agttgaaatc
     1081 gatgctgagt aataccgtca gttcttccag ctataaaacg ttggcgcaga ttggtatcac
     1141 gaccgatccc agcgatggca aactggaact ggatgccgac aaactcaccg ctgcactgaa
     1201 aaaagatgcc agcggcgtag gtgcattgat tgttggcgat ggtaaaaaaa ccggcatcac
     1261 gaccaccatc ggcagcaacc tgaccagttg gctttcgaca acgggcatta ttaaagccgc
     1321 taccgatggc gttagtaaga ccctgaataa attaactaaa gactacaacg ccgccagcga
     1381 tcgcattgat gcgcaggtcg ctcgctacaa agaacaattt acccaactgg acgttttaat
     1441 gacctcgtta aacagcacca gcagctactt aacgcagcag ttcgaaaaca acagtaattc
     1501 caagtaagca atattcatcg ggagacaggt catgtacgcg gcaaaaggca cccaggccta
     1561 tgcacaaatt ggcgtcgaaa gcgccgtaat gagcgccagc cagcagcagc tggtcaccat
     1621 gctatttgat ggagtgctga gcgcactggt tagagcgagc ctgtttatgc aggacaacaa
     1681 tcagcaaggc aaaggcgtct ctttgtcaaa agcgatcaac atcattgaga acggactgcg
     1741 ggtgagtctt gatgaagaga gcaaagacga actaacccaa aacttgattg ctctttatag
     1801 ctatatggtc aggcgcttgc tgcaagccaa tttacgcaac gatgtctccg cagtcgaaga
     1861 agtggaagca ttaatgcgca atattgccga tgcctggaaa gagtcgttac tctccccttc
     1921 tttgattcag gacccagtct gatgaaccat gcaccgcatt tatatttcgc ctggcaacaa
     1981 ctcgtcgaaa aaagccagct catgttacgc ctggcaacgg aagaacaatg ggacgaactc
     2041 atcgccagcg aaatggcgta tgtgaatgcg gtgcaggaga ttgcacattt gactgaagag
     2101 gttgacccgt ccaccacgat gcaggagcag ctccgcccga tgctgcgcct gattctcgac
     2161 aacgaaagca aggtaaagca gttattacag attcggatgg atgaactggc gaaactggtc
     2221 ggtcagtcat cggtgcaaaa atcggtgtta agtgcctatg gcgatcaggg cggctttgtg
     2281 ctggctccgc aggataacct cttttgaatc tgaatgagtc gatggctcgc gaataatccg
     2341 attacggcta cgcttctaat gttccccttg aatggagtcg aagaatgcgt aatcccacgc
//