From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: P.sativum gene for phenylalanine ammonia-lyase, complete cds.
Date: 9 Jan 93 05:47:27 GMT
Distribution: bionet
Checksum: 50825 9

LOCUS       PEAPAL2      4472 bp ds-DNA             PLN       09-JAN-1993
DEFINITION  P.sativum gene for phenylalanine ammonia-lyase, complete cds.
ACCESSION   D10003
KEYWORDS    phenylalanine ammonia-lyase.
SOURCE      Pisum sativum (sub_species Midoriusui) epicotyl DNA.
  ORGANISM  Pisum sativum
            Eukaryota; Plantae; Embryobionta; Magnoliophyta; Magnoliopsida;
            Rosidae; Rosales; Fabaceaea.
REFERENCE   1  (bases 1 to 4472)
  AUTHORS   Yamada,T., Tanaka,Y., Sriprasertsak,P., Kato,H., Hashimoto,T.,
            Kawamata,S., Ichinose,Y., Kato,H., Shiraishi,T. and Oku,H.
  TITLE     Phenylalanine ammonia-lyase genes from Pisum sativum : Structure
            organ-specific expression and regulation by fungal elicitor and
            suppressor
  JOURNAL   Plant Cell Physiol. 33, 715-725 (1992)
  STANDARD  full automatic
COMMENT     Submitted (21-NOV-1991) to DDBJ by: Tetsuji Yamada Laboratory of
            Plant Pathology & Genetic Engineeing College of Agriculture Okayama
            University 1-1 Tsushima-naka Okayama 700 Japan Phone:  0862-52-1111
            x 722 Fax:    0862-54-0714.
FEATURES             Location/Qualifiers
     CAAT_signal     1859..1865
     TATA_signal     1947..1953
     prim_transcript 1992..>4349
     exon            1992..2512
                     /number=1
     intron          2513..2602
                     /number=1
     exon            2603..>4349
                     /number=2
     CDS             join(2085..2512,2603..4349)
                     /EC_number="4.3.1.5"
                     /gene="PAL2"
                     /product="phenylalanine ammonia-lyase"
                     /codon_start=1
                     /translation="MEAIGAAITKNNSGYDSFCLTNAKNNNIKVSDSDPLNWGVAAEA
                     MKGSHLDEVKRMVDEYRKPVVRLGGETLTISQVAAIAAHDHGVKVELSESARAGVKAS
                     SDWVMESMNKGTDSYGVTTVHGATSHRRTKQGGALQKELIRFLNAGIFGNGSESTHTL
                     PHTATRAAMLVRINTLLQGYSGIRFEILEAITKLINNNVTPCLLRGTITASGDLVPLS
                     YIAGLLTGRPNSKAHGPSGEILNAREAFQSAGINDGFFELQPKEGLALVNGTAVGSGL
                     ASIVLFEANILAVLSEVLSAIFAEVMQGKPEFTDHLTHKLKHHPGQIEAAAIMEHILD
                     GSAYVKAAKKLHEMDPLQKPKQDRYALRTSPQWLGPLIEVIRFSTKSIEREINSVNDN
                     PLIDVSRNKALHGGNFQGTPIGVSMDNTRLALASIGKLLFAQFSELVNDFYNNGLPSN
                     LSASRNPSLDYGFKGSEIAMASYCSELQYLANPVTTHVQSAEQHNQDVNSLGLISSRK
                     TYEAIEILQLMSSTFLIALCQAIDLRHLEENLKNSVKNMVSHVAKRTLTTGINGELHP
                     SRFCEKDLLRVVDREHVFSYIDDPCSATYPLMQKLRQVLVDHALVNGESEKNLNTSIF
                     QKIATFEDELKTLLPKEVESARGAYENGNTTISNKIKECRSYPLYKFVREELGTSLLT
                     GEKVISPGEECDKLFTAICQGKIIDPLLECLGDWNGAPLPIS"
BASE COUNT     1377 a    764 c    848 g   1483 t
ORIGIN      
        1 taaaaagggt ataatcttgc atctattgac gtatgaactt gatattctga agagatttga
       61 gttaataaat tgtaagtctg caatcacacc tgctgaaaca aatcacaagt tggatttcga
      121 tgatgaggat gatgatgttg aggctacgat ttttaaatag ttggtaggct cattgaatac
      181 catacctgat atttgatatg cagttggaat gatgagtagg tttatgaaga aaccaaagtg
      241 gtcctattac caacacgttg tcaggatact gaggtatgtt aaggcaattc ttaagtatgg
      301 agttttattc ccttctggtg tggaaactaa gtcagagctg atatgttatt cagactctga
      361 ttggtgtgga gacagagtta attgaagaaa tacttttgga cattttttca agtatctgag
      421 aggtcctatt tcttggtgct ccaagagaca accagtggtt gtattgtcaa actgtgaagc
      481 tgagtacatt ttaggtgctt tgtctgctag tcaagttgta tggattctga acttgctgaa
      541 ggatctgaag atcaaggtgc gcaagtctgt gaagttgatg attgataaca aacaagctat
      601 aagtcttgcc aaaaatctat tgttgcatgg aagaagcaag cacattgaca tgaagtttca
      661 tttttcttgt tgaactaggt tcagaatggg cagcttgaaa ttgtgcattg tagtacccca
      721 gaagtagctg gctgatattc tgactaagac tatcaagact gagcatttta tccatttaag
      781 ggatgaaatt agtgttgttg attatagttc tgaatatgga ttaagggatg gtattggagt
      841 ccaatcaaat attcaattag catttcattt aattgttatt taagtttaat ttgagattgc
      901 atttagtttg aatattgttt ttaatgtgtg tataataaat actgaacttc tattcggtta
      961 gaaagtggcg taatagattt ttcattcaat agaaattagt tacaattttc tctcttctct
     1021 atcttccatc ttcatcatct tcatcttctt cctcttgtgc attaacaggg attgcaagct
     1081 gaacctgaat aaaaatttgg tgcacgaagg ttatggattg atcttgtgat aaaaactcaa
     1141 gttgatagta gaactcttaa gaaatatata ttatagattg taatagatag catgtttaag
     1201 gtcggagtaa cataaattct agtgatcatt ttcaaaactc ttttatttta ttttattgta
     1261 ttttattttc cataatcaaa tatatgctta aatcaattct ttttataaaa gaaatttcaa
     1321 aaattagtta acacaattca ctttccactg ttatttttta agtaacttta tcataatttt
     1381 tggttggatt ttttagagtt tctttgttat tatgttttgg ctagaaacaa tacattttct
     1441 tttagctatt gttattttct tctttaaata ttatcattta tttcttttgt ttcgtttaaa
     1501 aattgagaca ctctttaagt aaattaatga ttagagagga ttgaccaggt atagagttga
     1561 atgagatcaa gaatgatggt caaatcatgc atgtaatatc ttagtcagct ctctcgtggt
     1621 tgcttctcac caactaccta atttttgttt tggtccatac atatatatat ctaccaacca
     1681 tgaatcaaag aataacaaga tcttagagtg ccacgttgca atatcatagc cactcaacac
     1741 caagtgataa ttaatcaaac gctcctaatt aattctcaac caaaccactt tttgttttct
     1801 actattcctt gtttgtgcaa cttgtgtctc tttacctacc atccacatat ccacacatgc
     1861 aattgcaaga tagaaaaata attaataata ataataataa taataataat aataataata
     1921 atggagcctc caaaacaaac ctcttatata aagctcaact actcatcctt cacatctcaa
     1981 caaattcact acatacaact ctttcattct ttctttcttt cataaccatt ttatatttac
     2041 tcaaaattct agctatattc atctacaagt atcaattacg taacatggaa gcaataggtg
     2101 cagccattac aaaaaacaac agcggttacg attcattctg tttgaccaat gctaaaaaca
     2161 ataacatcaa agtgagtgac tctgatcctt tgaattgggg tgttgcggcg gaggcaatga
     2221 aaggtagtca cttggacgag gtgaaacgta tggtggatga gtaccggaag ccggtggtcc
     2281 gtcttggtgg tgagacgctc acaatttctc aggtagctgc cattgccgca catgatcatg
     2341 gtgtgaaggt ggagctgtca gaatctgcta gggctggcgt taaggcgagc agtgactggg
     2401 taatggagag tatgaacaaa ggcacagaca gttacggtgt caccaccgtt catggcgcca
     2461 cctctcaccg tcgaaccaaa caaggcggtg ctttgcagaa agaactcatt aggtaattac
     2521 atctctatct ccttatacta tcatttttct ttcatggact ttcataagaa atatgcttat
     2581 tcttttgaac tcttgttttc agatttttga atgctggaat atttggaaat ggaagtgagt
     2641 caactcatac actaccacac acagcaacaa gagctgctat gcttgtgaga atcaacacac
     2701 ttctccaagg ttattcagga attagatttg aaatcttgga agctataacc aaactcatta
     2761 acaacaacgt caccccatgt ttactccgtg gtacaatcac agcttctgga gatttagtcc
     2821 ctctttctta cattgctggt ttattaactg gaagaccaaa ttccaaagct catggaccgt
     2881 ctggagaaat acttaatgct agagaagctt ttcagtcagc tggaatcaat gatggtttct
     2941 ttgaattgca accgaaagaa ggtcttgcac ttgttaacgg aactgctgtt ggttctggtt
     3001 tagcttctat tgttctcttt gaagctaaca tattagctgt gttgtctgaa gtcctatccg
     3061 ctatttttgc tgaagttatg caagggaaac ctgagtttac tgatcatttg acacataagt
     3121 tgaagcacca tcctggtcaa attgaggctg ctgctattat ggaacacatt ttggatggaa
     3181 gtgcttatgt caaagctgct aagaagttgc atgagatgga tcctttgcag aagccaaaac
     3241 aagatagata tgcacttaga acttcaccac aatggcttgg tcctcttatt gaagtcatta
     3301 gattctctac taagtcaatt gagagggaga tcaactctgt taatgataac cctttgattg
     3361 atgtttcaag aaacaaggct ttgcatggtg gaaactttca aggaacacct attggtgtat
     3421 ccatggataa tacacgtttg gctcttgcgt caattggtaa actcttgttt gctcaattct
     3481 ctgaactcgt caatgatttt tacaacaacg ggttgccttc taatctctca gctagtagaa
     3541 atcccagctt ggattatgga ttcaagggat ccgaaattgc catggcttct tattgttcgg
     3601 agttacaata tcttgcgaac ccagttacaa ctcatgttca aagtgctgag caacacaacc
     3661 aagatgtgaa ctctttgggt ttgatatctt ctaggaaaac atatgaagcc attgagatcc
     3721 ttcagctcat gtcttcgaca ttcttgattg cactttgtca agcaattgat ttaagacatt
     3781 tggaggagaa cttgaaaaac tcagtcaaga atatggtgag ccatgttgcc aaaaggactc
     3841 ttactacagg tatcaatgga gaacttcatc cttcaagatt ttgtgaaaag gacttgctga
     3901 gagtggttga tagggaacat gtgttctcct atattgacga cccttgtagt gctacatatc
     3961 cattgatgca aaagctaagg caagtgttag tggaccatgc attagtaaat ggtgaatctg
     4021 agaagaattt gaacacatca atcttccaaa agattgcaac ttttgaggat gagttgaaga
     4081 ccctcttgcc aaaagaggtt gaaagtgcaa ggggtgcata cgagaatgga aatacaacaa
     4141 tttcaaacaa gatcaaagaa tgcagatcct atccacttta caagtttgtg agagaagaac
     4201 tgggaactag tttactaacc ggagaaaagg tcatttcacc cggtgaagaa tgtgataaat
     4261 tgttcacagc tatttgtcag ggaaaaatca tcgatcctct tcttgaatgc ttgggagact
     4321 ggaacggtgc tcctcttcca atttcttaac tttattgatt acttttaaaa gtatttcctt
     4381 tgtattcatg caagtgtagc aatattagat ttttcatgtc tttaacaaat taatatgaaa
     4441 agaaattcat tttaatttcc tccaatatca aa
//