From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Nicotiana sylvestris (clone 6PU11) hexameric polyubiquitin (ubi11)
Date: 31 Dec 92 01:41:16 GMT
Distribution: bionet
Checksum: 55659 6

LOCUS       TOBUBI11A    1606 bp ss-mRNA            PLN       17-DEC-1992
DEFINITION  Nicotiana sylvestris (clone 6PU11) hexameric polyubiquitin (ubi11)
            mRNA, complete cds.
ACCESSION   M74101
KEYWORDS    polyprotein; protein degradation; stress protein; ubiquitin.
SOURCE      Nicotiana sylvestris (library: lambda gt10) protoplast cDNA to
            mRNA.
  ORGANISM  Nicotiana sylvestris
            Eukaryota; Plantae; Embryobionta; Magnoliophyta; Magnoliopsida;
            Asteridae; Solanales; Solanaceae.
REFERENCE   1  (bases 1 to 1606)
  AUTHORS   Genschik,P., Parmentier,Y., Durr,A., Marbach,J., Criqui,M.C.,
            Jamet,E. and Fleck,J.
  TITLE     Ubiquitin genes are differentially regulated in protoplast-derived
            cultures of Nicotiana sylvestris and in response to various
            stresses
  JOURNAL   Plant Mol. Biol. 20, 897-910 (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     mat_peptide     39..266
                     /product="ubiquitin monomer"
                     /gene="ubi11"
                     /codon_start=1
     mat_peptide     267..494
                     /product="ubiquitin monomer"
                     /gene="ubi11"
                     /codon_start=1
     mat_peptide     495..722
                     /product="ubiquitin monomer"
                     /gene="ubi11"
                     /codon_start=1
     mat_peptide     723..950
                     /product="ubiquitin monomer"
                     /gene="ubi11"
                     /codon_start=1
     mat_peptide     951..1178
                     /product="ubiquitin monomer"
                     /gene="ubi11"
                     /codon_start=1
     mat_peptide     1179..1406
                     /product="ubiquitin monomer"
                     /gene="ubi11"
                     /codon_start=1
     3'UTR           1413..1606
                     /gene="ubi11"
                     /evidence="EXPERIMENTAL"
     5'UTR           1413..1606
                     /gene="ubi11"
                     /evidence="EXPERIMENTAL"
     polyA_signal    1594..1599
                     /gene="ubi11"
     CDS             39..1412
                     /gene="ubi11"
                     /evidence="EXPERIMENTAL"
                     /product="hexameric polyubiquitin"
                     /codon_start=1
                     /translation="MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLI
                     FAGKQLEDGRTLADYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVESSDTIDNV
                     KAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGGMQIFVKTL
                     TGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKEST
                     LHLVLRLRGGMQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGK
                     QLEDGRTLADYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVESSDTIDNVKAKI
                     QDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGGMQIFVKTLTGKT
                     ITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLV
                     LRLRGGF"
BASE COUNT      422 a    360 c    395 g    429 t
ORIGIN      
        1 ctattctctc cttttccgat tcccaaaaaa ctttcaagat gcaaatcttt gtaaagacgc
       61 tcaccggaaa aaccattact ctcgaggttg agagttccga cactattgat aatgtcaagg
      121 ctaagatcca ggacaaggaa ggaatccctc ccgatcagca gaggttgatc tttgctggaa
      181 agcagctaga ggatggccgc actcttgccg actacaacat ccaaaaggag tccacccttc
      241 accttgtcct ccgtctgcgc ggtggcatgc agatctttgt gaaaactttg actgggaaga
      301 ccatcaccct tgaggttgag agctctgaca ccattgacaa tgttaaggcg aagatccagg
      361 acaaggaagg catccctcca gaccagcaga ggttgatttt tgctggtaag cagcttgagg
      421 atggacgaac tcttgccgac tacaacattc agaaggaatc cacccttcac cttgtcctcc
      481 gtctgcgtgg tggcatgcag atttttgtga aaactttgac tgggaagacc atcacccttg
      541 aggttgagag ctccgacacc attgacaatg tgaaggcaaa gatccaggac aaggaaggca
      601 tccctccaga tcagcagagg ttgatttttg ctggtaagca gctcgaggat ggacgaactc
      661 ttgctgacta caacatccag aaggagtcca ccctccacct tgtcctccgt ctgcgtggtg
      721 gcatgcaaat ttttgtaaag accctcactg gcaagaccat cactctcgag gttgagagtt
      781 ccgacactat tgataatgtc aaggctaaga ttcaggataa ggaaggaatc cctcctgatc
      841 agcagaggct gatctttgct ggaaagcagc tagaggatgg ccgcactctt gctgactaca
      901 acattcagaa ggagtccact cttcaccttg tcctccgttt gcgcggtggc atgcagatct
      961 ttgtgaagac attgacaggg aagacaatca ccttggaggt cgaaagttct gatacaattg
     1021 ataatgtgaa ggcgaagatc caagataaag aagggattcc accggatcag cagcgattga
     1081 tctttgctgg taagcagctt gaggatggga ggacccttgc tgattataat atccagaagg
     1141 agtccacact gcatttggtg ctccgtctga ggggagggat gcagattttt gtcaagactc
     1201 taactgggaa gaccatcact ttggaggtgg agagctcaga tactattgat aatgtgaaag
     1261 caaagattca ggacaaggag ggtattccac cagatcagca gaggctgatt tttgctggta
     1321 agcagctgga ggatggtcgt acccttgcag attataacat ccagaaagaa tccacccttc
     1381 acctcgtgct tcgtctccgt ggtggctttt gagaagttaa ctcctccgtt taaggatctt
     1441 gaagacttct gtgctttcct ttagtggctt ttgttatgct ttgtgtttga actgttggtt
     1501 ttgtttgaat cctaagactt gcatgctatg ccttatatgg cattgattac gttctttctg
     1561 tcaagacagt tgtttttagc tgttggcttc gtaaataaat ttagtt
//