From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: D.pseudoobscura Adh gene for alcohol dehydrogenase
Date: 28 Dec 92 21:26:40 GMT
Distribution: bionet
Checksum: 43869 8

LOCUS       DROADHGN20   3480 bp ds-DNA             INV       01-DEC-1992
DEFINITION  D.pseudoobscura Adh gene for alcohol dehydrogenase
ACCESSION   X62200
KEYWORDS    ADH gene; alcohol dehydrogenase.
SOURCE      Drosophila pseudoobscura DNA.
  ORGANISM  Drosophila pseudoobscura
            Eukaryota; Animalia; Metazoa; Arthropoda; Uniramia; Insecta;
            Pterygota; Neoptera; Holometabola; Diptera; Brachycera;
            Cyclorrhapha; Schizophora; Drosophiloidea; Drosophilidae.
REFERENCE   1  (bases 1 to 3480)
  AUTHORS   Schaeffer,S.W. and Miller,E.L.
  TITLE     Estimates of gene flow in Drosophila pseudoobscura determined from
            nucleotide sequence analysis of the alcohol dehydrogenase region
  JOURNAL   Genetics 132, 471-480 (1992)
  STANDARD  full automatic
COMMENT     *source: strain=GB13; *source: isolate=Gundlach Bundschu Winery,
            Sonoma Valley, *source: California; *source: chromosome=IV section
            88;
               From EMBL    entry DPADHGN20;  dated 16-OCT-1992.
FEATURES             Location/Qualifiers
     mRNA            join(40..119,916..1021,1102..1506,1567..1982)
                     /note="adult mRNA (Adh)"
     mRNA            join(883..1021,1102..1506,1567..1982)
                     /note="larval mRNA (Adh)"
     promoter        8..14
                     /note="adult promoter (Adh)"
     prim_transcript 40..1982
                     /note="adult primary transcript (Adh)"
     exon            40..119
                     /note="adult exon 1a"
     intron          120..915
                     /note="adult intron"
     promoter        847..854
                     /note="larval promoter (Adh)"
     prim_transcript 883..1982
                     /note="larval primary transcript (Adh)"
     exon            883..1021
                     /note="larval exon 1"
     exon            916..1021
                     /note="adult exon 1b"
     intron          1022..1101
                     /number=1
     exon            1102..1506
                     /number=2
     intron          1507..1566
                     /number=2
     exon            1567..1982
                     /number=3
     polyA_signal    1959..1964
     polyA_site      1982
     CDS             join(929..1021,1102..1506,1567..1833)
                     /EC_number="1.1.1.1"
                     /gene="Adh"
                     /product="alcoholdehydrogenase"
                     /codon_start=1
                     /translation="MSLTNKNVVFVAGLGGIGLDTSRELVKRNLKNLVILDRIDNPAA
                     IAELKAINPKVTITFYPYDVTVPVAETTKLLKTIFAQVKTIDVLINGAGILDDHQIER
                     TIAVNYTGLVNTTTAILDFWDKRKGGPGGIICNIGSVTGFNAIYQVPVYSGSKAAVVN
                     FTSSLAKLAPITGVTAYTVNPGITKTTLVHKFNSWLDVEPRVAEKLLEHPTQTSQQCA
                     ENFVKAIELNKNGAIWKLDLGTLEPITWTQHWDSGI"
BASE COUNT      976 a    799 c    791 g    914 t
ORIGIN      
        1 cgtagtgtat ttaaggcact gcacatgtcg aggaccacga ttattgtcta cagtgcagtc
       61 agtagttgtc aggtgcagca ggcggaccag catttcttta aatctactca atcgatcaag
      121 taagtaactg taatccaagt acagtcggcc catcccactc ggttctgccg taactcaaga
      181 aatttcttga tagcgcagtt ccagttgtag aacgtcgttt aaaaaaacat ctcgttggag
      241 cttgggttga aaaacgttcg agttgggcgt agaaaagttc aaatataaaa tatcaaaaaa
      301 gagaaaatat cttcccaaag cagctgtcca ttttttgtta aagtgataga tcaaaaattc
      361 gtatgacgta tttcccaagt gctctttatg caaataattt cctcaattct aattctacaa
      421 gaagcacttt tggctcttgt ccagaaaaaa acaatttgtt tacacttttc ggttgataac
      481 agaagcggac ccacggctaa gtaacataac agtcgcctgt taagtaatgc cctcactctc
      541 tgttagatgg ccgcgcgtgt cgactccgat agaccagcag acacgcagta gttttcctca
      601 agaacctgaa ctctaaacat agacataatt tacgatacct tgagactcaa ataaaaatac
      661 atgtatgagt ggtattctat tcctgattag actcgcacac acacacgtac gagtattaac
      721 ataaatttgc cggcaaataa ccaaaacaaa acgaacgcaa acgatccgaa cacgtaatgc
      781 gagagataag aaacagaaaa gctcgacgtg aaagaagctt ctggcggaag ctcttgccga
      841 actccctata aatacgagac agaaaaccag aataaatata ataacaaact gttgaattgt
      901 cccatcgatt cccagatcaa aagacagaat gtctctcacc aacaagaacg tcgttttcgt
      961 ggccggtctg ggcggcattg gcctggacac cagccgggag ttggtcaagc gtaatctgaa
     1021 ggtaagagag atggagagag agagcaatcc cttttccatg gaatctatgg aatcctaaat
     1081 ttaaaaattt atttttttta gaacctggtc atcctggatc gcattgacaa tccggctgcc
     1141 attgccgaac tgaaggcaat caatcccaag gtgaccatca ccttctatcc ctacgatgtg
     1201 actgtgcccg tcgctgagac caccaagctc ctgaagacca tctttgccca ggtgaagaca
     1261 atcgatgtcc tgatcaacgg tgctggcatc ctggacgatc atcagatcga gcgcaccatt
     1321 gccgttaact acacgggcct ggtcaacacc accacagcca ttctggactt ctgggacaag
     1381 cgcaagggcg gcccaggcgg catcatttgc aacattggct ccgtcaccgg tttcaatgcc
     1441 atctaccagg tgcccgttta ctccggctcc aaggcggcgg tggttaactt tacctcctcc
     1501 ctggcggtaa gaaatcctac aactatccta ctccccaaaa ctaaatattt ctttgaaatc
     1561 ccatagaaac tggctcccat tactggtgtc actgcttaca ctgtcaatcc tggcatcact
     1621 aagaccactc tggtccacaa attcaactcg tggctggatg tggagccccg tgtggcagag
     1681 aagctgctcg agcatcccac ccagacctct cagcagtgcg ccgagaactt tgtgaaggcc
     1741 attgagctga acaagaacgg tgccatctgg aagttggatc tgggcacctt ggagcccatc
     1801 acatggaccc agcactggga ctcgggcatc taaaccatcc cagagactct atgggacatg
     1861 gcgttttagc ttttagtttc tttttttcca ctcaattgtt acgtatatac atacacatat
     1921 ggcaataagg ctgatttgaa ccccgttttg aatatgataa tacaaaatta tatttgagaa
     1981 atttcaacaa aatcgataag agctatagct ctcttttaat taaaaaaaac tagataaaaa
     2041 gagcaatggt cagtggtggt cagtggtcgt tgtggcctct atcgatttcc acacaaaaac
     2101 tttactgttt agtaatagaa aagaacttga gaaaggcagc caaaagaatg tacgatctga
     2161 cgggtaagca tgtctgctat gtggctgact gcggtggcat tgcactggag accagcaagg
     2221 ttctcatgac caagaatata gcggtgagtg gtagagtggt ggtgtagaga gtggttctgg
     2281 ttcttaaaca agtctctagt ctctagtctc ttggctgctg ctggtagctg tcgaaactaa
     2341 ttaagttaat gactttttca tgttttgttt ttgtccgctg acttttgctg ttcaaagcta
     2401 caattaagtt tatgttttga gtttgatcta cttttgctta agggaagtac tttcgattac
     2461 ggattgctgg catataaaaa tagataccaa ggaatgtgca tcatatcata ttgattctat
     2521 cttgattctt tgcccagaaa ctggccatcc ttcagagcgt ggaaaacccc ccggccatcg
     2581 ctcagttaca atcgattaag cacagcacac agatcttctt ctggaccttc gatgtgacca
     2641 tggcccgcga ggagatgaag aagtacttcg atgaggtcat ggtccagatg gactacatcg
     2701 atgtgctgat caatggggcc accctgtgcg atgagcggaa cattgatgcc accatcaata
     2761 cgaatctgac cggcatgata aacaccgtgg ccactgtgct gccctatatg gaccgtaaga
     2821 tgggcggttc gggtggactg attgtgaatg tcacctccgt cataggactg gatccatcgc
     2881 cagtcttttg tgcgtacagt gcctcaaagt ttggtgtgat tgggttcacc cgaagtctag
     2941 cggtgagtgg gcgagatctt aacttgaaaa tcctctctaa tgtcttccat tcttccgcag
     3001 gatcccctgt attacaccca aaatggtgtg gctgtcatgg ccgtctgctg tggccccacc
     3061 aaagtgtttg tcgatcgcga actgactgcc ttcctggaat acggccaaac ctttgccgat
     3121 cgcctgcgcc gtgccccctg ccaatcgacg gccgtctgcg gcaagaatat agttaatgcc
     3181 atcgagagat cggagaacgg acagatctgg attgccgaca agggcggcct ggagtcggtg
     3241 gccctccact ggtattggca tatggccgat cagtttgtga actatatgca gagcactgac
     3301 gatgaggatc aggagttttt tttgggccag cgatgaggca tgccttgatc cacggaataa
     3361 aacatcaaat gtgtcacaaa aagtaggcaa cttgtttgtg ggtctggaaa tagatttgaa
     3421 atattttgag gtcatatgaa taatgacata aatgagggtg cctgaaagta tgccaagttt
//