From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: P. paucimobilis gene for C alpha-dehydrogenase, complete cds.
Date: 15 Sep 92 10:05:10 GMT
Distribution: bionet
Checksum: 58370     4

LOCUS       PSELIGD      1229 bp ds-DNA             BCT       15-SEP-1992
DEFINITION  P. paucimobilis gene for C alpha-dehydrogenase, complete cds.
ACCESSION   D11341
KEYWORDS    C alpha-dehydrogenase;
            C alpha-dehydrogenation of arylglycerol-beta-aryl ether; cytosol;
            lignin degradation; oxido-reductase.
SOURCE      Pseudomonas paucimobilis (strain SYK-6) (library: pVK100) DNA,
            clone pBE10.
  ORGANISM  Pseudomonas paucimobilis
            Prokaryotae; Gracilicutes; Scotobacteria; Pseudomonadaceae.
REFERENCE   1  (bases 1 to 1229)
  AUTHORS   Masai,E.
  JOURNAL   Unpublished (1992)
  STANDARD  full staff_review
COMMENT     These data kindly submitted in computer readable form by:
            Eiji Masai
            Laboratory of Wood Chemistry
            Faculty of Agriculture
            Tokyo Noko University
            3-5-8 Saiwai-cho
            Fuchu-shi, Tokyo 183
            Japan
            Phone:  0423-34-6899
            Fax:    0423-34-5700
FEATURES             Location/Qualifiers
     RBS             139..142
     CDS             149..1066
                     /product="C alpha-dehydrogenase"
                     /gene="ligD"
                     /evidence=EXPERIMENTAL
                     /codon_start=1
                     /translation="MKDFQDQVAFITGGASGAGFGQAKVFGQAGAKIVVADVRAEAVE
                     KAVAELEGLGITAHGIVLDIMDREAYARAADEVEAVFGQAPTLLSNTAGVNSFGPIEK
                     TTYDDFDWIIGVNLNGVINGMVTFVPRMIASGRPGHIVTVSSLGGFMGSALAGPYSAA
                     KAASINLMEGYRQGLEKYGIGVSVCTPANIKSNIAEASRLRPAKYGTSGYVENEESIA
                     SLHSIHQHGLEPEKLAEAIKKGVEDNALYIIPYPEVREGLEKHFQAIIDSVAPMESDP
                     EGARQRVEALMAWGRDRTRVFAEGDKKGA"
BASE COUNT      247 a    356 c    422 g    204 t
ORIGIN
        1 gttgacattg tccggaaatg agtggaaacc cgcttcaagc agatggcgcg ggcatcgttg
       61 ccggcgcccg tcatgggatc gcccatgaca ggccggtcaa ccggttggtt ggcgcgtcat
      121 cggccgtaga aatcaggaga gggtcggaat gaaggatttc caggatcagg tcgcgttcat
      181 cacgggcggc gcgtcgggcg ccggcttcgg ccaggcgaaa gtgttcggtc aggcaggcgc
      241 gaagatcgtg gtggcggacg tgcgggccga agcggtcgag aaggccgtcg ccgagctgga
      301 agggctcggg atcaccgcgc atggcatcgt gctcgatatc atggaccgcg aggcctatgc
      361 ccgggcggcg gacgaagtgg aggccgtgtt cggccaggcg ccgacgcttc tctccaacac
      421 cgctggcgtg aacagcttcg ggccgatcga gaagaccact tatgatgatt tcgactggat
      481 catcggcgtc aatctgaacg gcgtcatcaa cggcatggtg accttcgtgc cgcgcatgat
      541 cgcgagcggg cggccggggc acatcgtcac cgtctcgtcg ctcggcggct tcatggggag
      601 cgcgctcgcc gggccctatt cggcggccaa ggcggccagc atcaatctga tggaaggcta
      661 tcggcagggg ctagagaaat acggcatcgg cgtctccgtc tgcacgccgg ccaacatcaa
      721 gtcgaacatc gcggaagcct cgcgcctgcg tcccgcgaaa tacggcacca gcggctatgt
      781 ggagaacgag gaatcgattg cctcgctgca ctccattcac cagcacgggc tcgagccgga
      841 gaagctggcg gaagcgatca agaagggtgt cgaggacaat gctctctaca tcattcccta
      901 tcccgaagtg cgcgaaggac tggagaagca ttttcaggcc atcatcgatt cggtcgcgcc
      961 gatggagagc gatccggaag gcgcccgcca gcgggtcgag gcactgatgg cctggggacg
     1021 ggaccgcacg cgggtcttcg ccgagggcga caagaaaggc gcctgacgaa atgcacgggc
     1081 gtggcggctt aaccgccgcg cccttttcgc tcatgacgag atgacaagag atctgggaga
     1141 caggaatgac gttgaaactc tacagcttcg gtcccggggc gaactcgctc aagccgctcg
     1201 cgacgctcta cgagaagggc ctcgaattc
//