From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: A. shirousamii mRNA for alpha-amylase, complete cds.
Date: 21 Dec 92 20:38:54 GMT
Distribution: bionet
Checksum: 38448 5

LOCUS       ASNAMY       1721 bp ss-mRNA            PLN       20-NOV-1992
DEFINITION  A. shirousamii mRNA for alpha-amylase, complete cds.
ACCESSION   D10461 D11380
KEYWORDS    alpha-amylase; starch inducible.
SOURCE      Aspergillus shirousamii (strain RIB 2504) cDNA to mRNA.
  ORGANISM  Aspergillus shirousamii
            Eukaryota; Fungi; Eumycota; Ascomycotina; Plectomycetes;
            Eurotiales; Trichocomaceae.
REFERENCE   1  (bases 1 to 1721)
  AUTHORS   Shibuya,I., Tamura,G., Ishikawa,T. and Hara,S.
  TITLE     Cloning of the alpha-Amylase cDNA of Aspergillus shirousamii and
            its expression in Saccharomyces cerevisiae
  JOURNAL   Biosci. Biotechnol. Biochem. 56, 174-179 (1992)
  STANDARD  full automatic
COMMENT     Submitted (20-Jan-1992) to DDBJ by: Ichiro Shibuya Institute for
            Production Research and Development 967 Azamatsuyama Kashiwa, Chiba
            277 Japan Phone:  0471-72-3900 Fax:    0471-75-0290.
FEATURES             Location/Qualifiers
     sig_peptide     41..103
                     /codon_start=1
     mat_peptide     104..1537
                     /product="alpha-amylase"
                     /codon_start=1
     polyA_site      1721
     CDS             41..1540
                     /EC_number="3.2.1.1"
                     /gene="AMY"
                     /product="alpha-amylase"
                     /codon_start=1
                     /translation="MMVAWWSLFLYGLQVAAPALAATPADWRSQSIYFLLTDRFARTD
                     GSTTATCNTADQKYCGGTWQGIIDKLDYIQGMGFTAIWITPVTAQLPQTTAYGDAYHG
                     YWQQDIYSLNENYGTADDLKALSSALHERGMYLMVDVVANHMGYDGAGSSVDYSVFKP
                     FSSQDYFHPFCFIQNYEDQTQVEDCWLGDNTVSLPDLDTTKDVVKNEWYDWVGSLVSN
                     YSIDGLRIDTVKHVQKDFWPGYNKAAGVYCIGEVLDVDPAYTCPYQNVMDGVLNYPIY
                     YPLLNAFKSTSGSMDDLYNMINTVKSDCPDSTLLGTFVENHDNPRFASYTNDIALAKN
                     VAAFIILNDGIPIIYAGQEQHYAGGNDPANREATWLSGYPTDSELYKLIASANAIRNY
                     AISKDTGFVTYKNWPIYKDDTTIAMRKGTDGSQIVTILSNKGASGDSYTLSLSGAGYT
                     AGQQLTEVIGCTTVTVGSDGNVPVPMAGGLPRVLYPTEKLAGSKICSSS"
BASE COUNT      428 a    458 c    425 g    410 t
ORIGIN      
        1 ctctcccttc tctgaacaat aaaccccaca gaaggcattt atgatggtcg cgtggtggtc
       61 tctatttctg tacggccttc aggtcgcggc acctgctttg gctgcaacgc ctgcggactg
      121 gcgatcgcaa tccatttatt tccttctcac ggatcgattt gcaaggacgg atgggtcgac
      181 gactgcgact tgtaatactg cggatcagaa atactgtggt ggaacttggc agggcatcat
      241 cgacaagttg gactatatcc agggaatggg cttcacagcc atctggatca cccccgttac
      301 agcccagctg ccccagacca ccgcatatgg agatgcctac catggctact ggcagcagga
      361 tatatactct ctgaacgaaa actacggcac tgcagatgac ttgaaggcgc tctcttcggc
      421 ccttcatgag agggggatgt atcttatggt cgatgtggtt gctaaccata tgggctatga
      481 tggagcgggt agctcagtcg attacagtgt gtttaaaccg ttcagttccc aagactactt
      541 ccacccgttc tgtttcattc aaaactatga agatcagact caggttgagg attgctggct
      601 aggagataac actgtctcct tgcctgatct cgataccacc aaggatgtgg tcaagaatga
      661 atggtacgac tgggtgggat cattggtatc gaactactcc attgacggcc tccgtatcga
      721 cacagtaaaa cacgtccaga aggacttctg gcccgggtac aacaaagccg caggcgtgta
      781 ctgtatcggc gaggtgctcg acgttgatcc ggcctacact tgtccctacc agaacgtcat
      841 ggacggcgta ctgaactatc ccatttacta tccactcctc aacgccttca agtcaacctc
      901 cggcagcatg gacgacctct acaacatgat caacaccgtc aaatccgact gtccagactc
      961 aacactcctg ggcacattcg tcgagaacca cgacaaccca cggttcgctt cttacaccaa
     1021 cgacatagcc ctcgccaaga acgtcgcagc attcatcatc ctcaacgacg gaatccccat
     1081 catctacgcc ggccaagaac agcactacgc cggcggaaac gaccccgcga accgcgaagc
     1141 aacctggctc tcgggctacc cgaccgacag cgagctgtac aagttaattg cctccgcgaa
     1201 cgcaatccgg aactatgcca ttagcaaaga tacaggattc gtgacctaca agaactggcc
     1261 catctacaaa gacgacacaa cgatcgccat gcgcaagggc acagatgggt cgcagatcgt
     1321 gactatcttg tccaacaagg gtgcttcggg tgattcgtat accctctcct tgagtggtgc
     1381 gggttacaca gccggccagc aattgacgga ggtcattggc tgcacgaccg tgacggtggg
     1441 ttcggatgga aatgtgcctg ttcctatggc aggtgggcta cctagggtat tgtatccgac
     1501 tgagaagttg gcaggtagca agatctgtag tagctcgtga agggtggaga gtatatgatg
     1561 gtactgctat tcaatctggc attggacagt gagtttgagt ttgatgtaca gttggagtcg
     1621 ttactgctgt catcccctta tactctttga ttgtttttcg aaccctaata ccaagcacgc
     1681 tagtctatta taagaaagaa tccagattaa tatgttttta t
//