From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: La Crosse virus M RNA for polyprotein, complete cds.
Date: 21 Jul 92 10:03:47 GMT
Distribution: bionet
Checksum: 34776     9

LOCUS       BLCMRP       4526 bp ss-RNA             VRL       21-JUL-1992
DEFINITION  La Crosse virus M RNA for polyprotein, complete cds.
ACCESSION   D10370 D00202
KEYWORDS    M RNA; polyprotein.
SOURCE      Bunyavirus La Crosse (individual_isolate 74-32813) cDNA to genomic
            RNA.
  ORGANISM  Bunyavirus La Crosse
            Viridae; ss-RNA enveloped viruses; Negative strand RNA viruses;
            Bunyaviridae; Bunyavirus.
REFERENCE   1  (bases 1 to 4526)
  AUTHORS   Grady,L.J., Sanders,M.L. and Campbell,W.P.
  TITLE     The sequence of the M RNA of an isolate of la cross virus
  JOURNAL   J. Gen. Virol. 68, 3057-3071 (1987)
  STANDARD  full staff_review
COMMENT     The middle-size (M) genomic RNA of a New York State, U.S.A. isolate
            of La Crosse virus has been cloned by a random priming procedure
            and its nucleotide sequence determined by the dideoxy method.
FEATURES             Location/Qualifiers
     CDS             62..4387
                     /product="M gene polyprotein"
                     /codon_start=1
                     /translation="MIRMLVLIVVTAASPVYQRCFQDGAIVKQNPSKEAVTEVCLKDD
                     VSMIKTEARYVKNATGVFSNNVAIRKWLVSDWHDCRPKKIVGGHINVIEVGDDLSLHT
                     ESYVCSADCTIGVDKETAQVRLQTDTTNHFEIAGTTVKSGWFKSTTYITLDQTCEHLK
                     VSCAPKSVQFHACFNQHMSCVRFLHRTILPGSIANSICQNIEIIILVTLTLLIFILLS
                     ILSKTYICYLLMPIFIPIAYMYGVIYNKSCKKCKLCGLVYHPFTECGTHCVCGARYDT
                     SDRMKLHRASGLCPGYKSLRAARVMCKSKGPASILSIITAVLVLTFVTPINSMVLGES
                     KETFELEELPDDMLEMALRINSYYFTCILNYAVSWGLIIAGLLVGLIFKKYQHRFLNI
                     YAMYCEECNMYHDKSGLKRHGDFTNKCRQCTCGQYEDATGLITHRKTYNCLVQYKAKW
                     MMNFLIIYIFLILIKDSAIVGQATGTDFTTCLETESINWNCTGPFLNLGNCQKQQKKE
                     PYTNIATQLKGLKAISVLDIPIITSIPDDIAGALRYIEEKEDFHVQLTTEYAMLSKYC
                     DYYTQFSDNSGYSQTTWRVYLRSHDFEACILYPNQHFCKCVKNGEKCSSSNWDFANGM
                     KNYYSGKQAKFDKDLNLALTALHHAFRGTSSAYIAAMLSKKSNDDLIAYTNKIKAKFP
                     GNALLKAIIDYIAYMKGLPEMANFKYDEFWDELLYKPNPAKASNLARGKESSYNFKLA
                     ISSKSIKTCKNVKDVACLSPRSGAIYSSIIACGEPNGPSVYRKPSGGVFQSSTDRSIY
                     CLLDSHCLEEFEAISQEELDAVKKSKCWEIEYPDVRPLQESDGAKSCRMKDSGNCNVA
                     TNRWPVMQCENDKFYYSELQKDYDKTQDIGHYCLSPGCTTIRYPINPKHISNCNWQVS
                     RSSIAKIDVHNVEDIEQYKKAITQKLQTSLSLFKYAKTKNLPHIRPIYKYITMKETET
                     AEGIESAYIESEVPALAGTSVGFKINSKEGKHLLDVIAYVKSASYSSVYAKLYSTGPT
                     SGINTKHDELCTGPCPANINHQVGWLTFARERTSSWGCEEFGCLAVSDGCVFGSCQDI
                     IKEELSVYRKETEEVTNVELCLTFSDKTYCTNLNPVTPIITDLFEVQFKTVETYSLPR
                     IVAVQNHEIKIGQINDLGVYSKGCGNVQKVNGTVYGNGVPRFDYLCHLASRKEVIVRK
                     CFDNDYQACKFLQSPASYRLEEDSGTVTIIDYKKILGTIKMKAILGDVKYKTFADSVD
                     ITAEGSCAGCINCFQNIHCELTLHTTIEASCPIKSSCTVFHDRILVTPNEHKYALKIV
                     CTEKPGNTLTIKVCNTRIEASMALVDAKPIIELAPVDQTAYIREKDERCKTWMCRVRD
                     EGLQVILEPFKNLFGSYIGIFYTFIISIIALLVIIYVLLPICFKLRDTLRKHDDAYKR
                     EMKIR"
BASE COUNT     1549 a    788 c    929 g   1260 t
ORIGIN
        1 agtagtgtac taccaagtat agataacgtt taaatattaa agttttggat caaagccaaa
       61 gatgattcgc atgctggtgc tgattgtagt tacggctgca agcccagtgt atcagagatg
      121 tttccaagat ggggctatag tgaagcaaaa cccatccaaa gaggcagtca cagaagtgtg
      181 cctaaaagat gatgttagca tgatcaaaac agaggccagg tatgtaaaaa atgcaacagg
      241 agttttctca aacaatgttg caataaggaa gtggttagtc tctgattggc atgactgcag
      301 acctaagaag atcgttgggg ggcacatcaa tgtaatagaa gttggtgatg acctgtcgct
      361 tcatactgaa tcatatgttt gcagtgcaga ttgtaccata ggtgtagaca aggaaacagc
      421 acaggtcaga cttcaaacag ataccacaaa ccattttgaa atagcaggta ctactgtgaa
      481 gtcaggatgg ttcaaaagca caacatatat aactctagat cagacctgtg aacacctcaa
      541 agtttcctgc gccccaaaat ctgtacagtt ccatgcctgc ttcaatcagc atatgtcctg
      601 tgtcagattc ttacacagga caatattgcc tggttccata gctaattcca tatgtcagaa
      661 tattgaaatc ataattttag ttacacttac cttattaatc ttcatactgt taagcatttt
      721 aagtaagact tatatatgtt atttgttaat gcccatattc atccccatag catatatgta
      781 cggtgtaatt tacaataagt catgcaaaaa gtgcaagcta tgcggcctag tgtatcatcc
      841 attcacagag tgtggcacgc attgtgtctg tggtgctcgc tatgatactt ccgatagaat
      901 gaaactacac agagcttctg gattgtgccc tggttataaa agcctaagag ctgccagagt
      961 catgtgcaaa tcaaaagggc ctgcatcaat attgtctata attactgcag tgctggttct
     1021 aacctttgtg acaccaatca actccatggt tttaggggaa agtaaggaaa cctttgaact
     1081 agaagagctt ccagacgaca tgctggaaat ggcattaagg ataaattctt attacttcac
     1141 ctgtatctta aattatgctg taagctgggg tcttattatt gctggattat tggttggact
     1201 gatatttaaa aaataccagc ataggttctt aaatatttat gcaatgtact gtgaagaatg
     1261 taacatgtat catgacaaat ctgggctgaa gagacacggt gacttcacca acaaatgtag
     1321 acaatgcaca tgtggtcaat atgaagatgc tacaggtttg ataactcata ggaaaaccta
     1381 taactgttta gtgcagtaca aagcaaagtg gatgatgaat ttcctaataa tttacatatt
     1441 cttaattttg atcaaagatt ctgccatagt tggacaagct actgggacag atttcaccac
     1501 ctgcctagag actgagagta taaattggaa ctgcactggg ccattcctaa accttggaaa
     1561 ctgccaaaag caacaaaaga aagaacctta cacaaatatt gcaacccagc taaaagggct
     1621 aaaagcaatt tccgtactag atatccctat aattacgagt atcccagacg acattgcagg
     1681 tgctttaaga tatatagagg aaaaggaaga tttccatgtc caactaacta cagaatatgc
     1741 aatgctgagt aagtactgtg actattatac ccagttctca gataactcag gatatagtca
     1801 aacaacatgg agagtatact tgagatctca tgattttgag gcatgcatac tatatccaaa
     1861 tcagcatttc tgcaagtgtg tgaaaaatgg tgaaaagtgt agcagctcca attgggactt
     1921 tgccaatgga atgaagaatt attactctgg gaaacaagca aaatttgata aagatttaaa
     1981 cctagctcta acagctttgc atcatgcctt cagagggacc tcatctgcat atatagcagc
     2041 aatgctctca aagaagtcca atgatgattt gattgcatat acaaataaga taaaagcaaa
     2101 atttccaggt aatgcactat tgaaggctat aatagattat atagcataca tgaaaggttt
     2161 gccagaaatg gcaaatttca aatatgacga gttctgggat gaattattgt acaaacctaa
     2221 cccagcaaaa gcctcaaatc ttgctagggg aaaagagtca tcctacaact tcaagttagc
     2281 aatttcgtca aaatctataa aaacctgtaa gaatgttaag gatgttgcct gcttatcacc
     2341 aaggtcaggt gctatatact cttcaataat tgcatgcggt gaacccaacg ggccaagtgt
     2401 gtatagaaaa ccatcaggtg gtgtatttca atccagtact gaccggtcta tatattgttt
     2461 gctagacagc cattgtctag aggagtttga ggctatcagt caagaggaac tggatgcagt
     2521 aaaaaaatcc aagtgttggg agattgaata tcctgacgta agacccctcc aagaaagtga
     2581 cggggctaaa agctgtagaa tgaaagattc tgggaattgt aatgttgcaa ctaacagatg
     2641 gccagtaatg caatgcgaga atgacaaatt ttattattca gaacttcaaa aagattatga
     2701 caaaactcaa gatattggtc attattgctt aagccctgga tgtaccacta tccggtaccc
     2761 tattaatcca aagcatatct ctaattgtaa ttggcaggta agcagatcca gcatagcaaa
     2821 gatagacgta cataatgttg aagatattga acaatataaa aaggctataa cccaaaagct
     2881 tcaaacaagc ttgtctctat tcaagtatgc aaaaacaaaa aacttgccgc acattagacc
     2941 aatttataaa tacataacta tgaaggaaac agaaactgca gaagggatag agagcgcata
     3001 tattgaatca gaagtgcctg cattggctgg gacatctgtt gggttcaaaa ttaattctaa
     3061 agagggcaaa cacttgctag atgttatagc atatgtaaaa agtgcatcgt actcttcagt
     3121 atatgcgaaa ttatactcaa ctggcccaac atcaggaata aatactaaac atgatgaatt
     3181 gtgtactggc ccatgcccag caaatatcaa tcatcaagtt gggtggctca catttgcaag
     3241 agagaggaca agttcatggg gatgcgaaga gtttggttgc ctggctgtaa gtgatgggtg
     3301 cgtgttcggg tcatgccaag acataataaa agaagaacta tctgtctata ggaaggagac
     3361 cgaagaagtg accaatgttg agctatgttt aacattttca gacaagacat attgcacaaa
     3421 cttaaaccct gttaccccta ttataacaga tctatttgag gtacagttta aaactgtaga
     3481 gacttacagc ttgcccagaa ttgttgccgt gcaaaaccat gagattaaga ttgggcaaat
     3541 aaatgatcta ggggtttact ctaagggatg tgggaatgtt caaaaggtta atggaactgt
     3601 ttatggcaat ggggtcccca gatttgatta cttatgtcac ttagctagta gaaaggaagt
     3661 catcgttaga aaatgctttg ataatgatta ccaagcatgc aaattccttc aaagccctgc
     3721 tagttataga cttgaagaag acagcggtac tgtgactata attgattata agaaaatatt
     3781 aggcacaatc aagatgaagg caattttagg agatgtcaaa tacaaaacat ttgctgacag
     3841 tgttgatata actgcagaag ggtcgtgtgc tggctgtatc aattgctttc agaatattca
     3901 ttgcgaatta acgctgcata ccacaattga agccagctgc ccaattaaaa gttcttgcac
     3961 agtattccat gataggattc ttgtaacccc aaatgaacac aaatatgcat taaaaattgt
     4021 atgcacagaa aagccaggga atacactcac cattaaagtc tgcaatacta gaattgaagc
     4081 ttcaatggcc cttgttgatg caaagcctat catagaatta gcaccagttg atcagacggc
     4141 atatataaga gaaaaagatg aaagatgcaa aacttggatg tgtagggtaa gggatgaagg
     4201 gctgcaggtt atcttagagc catttaaaaa cttgtttgga tcttatattg ggatatttta
     4261 cacatttatt atatctataa tagcattgtt ggttatcatc tatgtactac tgcctatatg
     4321 cttcaaatta agggataccc ttagaaagca cgatgatgca tataagagag agatgaaaat
     4381 tagataaggg attcatgtaa aacagaattg agtcctgtat tatatattct atttgtagta
     4441 tagctgttgt taagtggggg gtggggaact aacaacagcg taagtttatt ttgcaaacat
     4501 tattttatac ttggtagcac actact
//