From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: S.suis mrp gene for 136-kDa surface protein
Date: 30 Jul 92 10:02:25 GMT
Distribution: bionet
Checksum: 17508     8

LOCUS       STRMRP136    4121 bp ds-DNA             BCT       30-JUL-1992
DEFINITION  S.suis mrp gene for 136-kDa surface protein
ACCESSION   X64450
KEYWORDS    mrp gene; surface-associated protein; virulence factor.
SOURCE      Streptococcus suis DNA.
  ORGANISM  Streptococcus suis
            Prokaryotae; Firmicutes; Firmibacteria; Gram-positive cocci;
            Streptococcaceae.
REFERENCE   1  (bases 1 to 4121)
  AUTHORS   Smith,H.E., Vecht,U., Gielkens,A.L. and Smits,M.A.
  TITLE     Cloning and nucleotide sequence of the gene encoding the 136-kDa
            surface protein (MRP) of Streptococcus suis type 2
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
COMMENT     *source: strain=serotype 2, strain: D282;
            From EMBL    entry SSMRP136;  dated 03-JUN-1992.
FEATURES             Location/Qualifiers
     promoter        4..34
     promoter        40..68
     RBS             147..152
     CDS             159..3929
                     /product="MRP"
                     /gene="mrp"
                     /codon_start=1
                     /translation="MRRSNKKSFDWYGTKQQFSIRKYHFGAASVLLGVSLVLGAGAQV
                     VKADETVASSEPTIASSVAPASTEAVAEEAEKTNAENTSAVATTSTEVEKAKAVLEQV
                     TSESPLLAGLGQKELAKTEDATLAKAIEDAQTKLAAAKAILADSEATVEQVEAQVAAV
                     KVANEALGNELQKYTVDGLLTAALDTVAPDTTASTLKVGDGEGTLLDSTTTATPSMAE
                     PNGAAIAPHTLRTQDGIKATSEPNWYTFESYDLYSYNKNMASSTYKGAEVDAYIRYSL
                     DNDSSTTAVLAELVSRTTGDVLEKYTIEPGESVTFSHPTKVNANNSNITVTYDTSLAS
                     ANTPGALKFSANDDVYSTIIVPAYQINTTRYVTESGKVLATYGLQTIAGQVVTPSSVR
                     VFTGYDYVATTTKAVQGPYPKGTVYLAGTVQKDTVQYKVIREIVENDQAVLKFYYLDP
                     TYKGEVDWRGTDTTGFIELLTTSPTTYKVGTIYDYNINSKITAPFTIDPTKNVMVFKE
                     SEQNEQGSKYRVIAQWSGDETTKGIYGKIYIATQVWTTKLGTNEWGWFDYSDDQAGIK
                     FNNKGFWPAGVQNTLRNATPATAVETTYIYKESSKYGDVIVEYYDTDGKQIVNSVVDT
                     PKSALGTEYNTDVDRRPASLVAADGTVYFYKEVKSDSAKTTGTVVAGTTTVKYVYEKA
                     GSVNVNFVDINGKVIKAPVSDEKDAKPGYNYDTDLDQKLASITFEGKEYKLVPAGDYP
                     VGKVGKGNNLIEVGNNTAKGIDPTTGKIEAGVNKEVTYVYRAVTGSVVVNYKDTEGNV
                     IKDPETDVSDAPVGDAYTTTDKKPNEIITKDGSRYVLVPSKTDGEENGKVIEGTITVT
                     YVYQKVANWIPEIPNVPETDRPKVPYPFDPTEPDEPIDPTTPGTNGEVPNIPYVPGYT
                     PVDPKDNTPLKPIDPNDPGKGYVPPTPENPGVDTPIPYVPVKKVVTNHVDEEGNPIAP
                     QEEGTKPNKSIPGYEFTGKTVTDEDGNTTHIYKKTPEVKNGTVVVNYVTEDGTVIKEP
                     VTDTPTSPEGTPYDTTDNKPKTITFKGEEYELVRVDGTENGKVVEGETVVTYVYRKVE
                     TPAKKVVTNHVDEEGNPVAPQEEGTKPNKSIPGYEFTGKTVTDEDGNTTHIYKKTPAK
                     KVVTNHVDEEGNPIAPQEDGTTPKRQISGYEYVRTVVDEEGNTTHIYRKLSNKPTTPE
                     KETPAKPQAGKTASGKAQLPNTGEASSVAGALGTAMLVATLAFARKRRRNED"
     terminator      4069..4098
BASE COUNT     1396 a    770 c    880 g   1075 t
ORIGIN
        1 gaattcataa tgtttttttg aggaatttta taatattact tggcatttaa agttatttgt
       61 agtataatac ctcgaatgat tgcgggagtt ttcaaggctt tgatacaaag agtagaaaat
      121 ttgtgtaatt aaattaatat ttatatgggg gattttttat gcgtagatca aataaaaaat
      181 catttgactg gtacggtacg aaacaacaat tttcgattcg taagtatcat tttggggcag
      241 caagcgtttt gcttggtgtg tcgttagttt taggtgctgg tgcacaggtt gttaaggctg
      301 atgaaactgt tgcttcatca gaaccaacta ttgccagtag tgtagcgcct gcttcaacag
      361 aagcggttgc agaagaagca gaaaaaacaa atgctgaaaa tacgagtgca gtagctacga
      421 cttcaacaga agttgaaaaa gcgaaagctg ttcttgaaca ggtaacatca gaatcaccac
      481 ttttggctgg tcttggtcaa aaagagttgg ctaaaactga agatgcaact cttgcaaaag
      541 ctatagagga tgctcaaaca aaacttgcag cagctaaggc aattttggct gactcagaag
      601 caactgttga gcaagttgaa gcgcaagtcg cagcggttaa agtagccaac gaggcgctag
      661 ggaatgaatt gcaaaaatac actgtagatg gtctcttgac agcggctctt gatacagtag
      721 cacctgatac aactgcatca acattgaaag ttggtgatgg cgaaggtacc cttctagata
      781 gcactacaac agcaacgcct tcaatggctg agccaaatgg tgcagcaatt gctccacata
      841 cacttcgaac tcaagatgga attaaagcga catcagagcc aaattggtat acttttgaat
      901 cgtacgattt gtactcatat aataaaaata tggctagctc aacttataaa ggagctgaag
      961 ttgatgccta cattcgttac tctttggata atgattcgtc aacaactgct gttttagcag
     1021 agttggtaag taggacaact ggtgatgtgt tagagaaata tacgattgaa ccgggcgaga
     1081 gtgttacgtt ttcacatccg acaaaagtta atgctaataa tagcaatata actgtgactt
     1141 atgatacctc attagcttct gctaatactc ctggagcatt gaaattctct gctaatgatg
     1201 atgtttattc aacaattatt gtacctgctt atcagattaa tacaactcgt tacgtcactg
     1261 aaagtggcaa agttttggca acctatggtc ttcaaactat tgcaggacag gtagttactc
     1321 catcttctgt tcgtgtattt actgggtatg attatgtggc aactacaact aaagccgttc
     1381 aaggtccata tccaaaggga acggtatacc ttgctggtac ggttcaaaag gatacagtac
     1441 aatataaagt tattcgtgaa attgtggaga acgaccaagc agttcttaaa ttctattatt
     1501 tagatcctac ctataagggt gaagtagatt ggagaggaac tgatacgact gggtttattg
     1561 agttgcttac aacttcccca acaacctata aagttggtac tatatacgat tacaatatta
     1621 attcaaaaat tacagctcca tttactattg atcctaccaa gaatgttatg gttttcaagg
     1681 aaagtgaaca gaacgagcaa ggtagcaaat atcgcgtcat tgctcaatgg tcaggagatg
     1741 aaaccactaa aggtatatat ggaaaaatct atatcgctac tcaggtttgg acgactaaat
     1801 tgggaacaaa cgagtgggga tggtttgact attctgatga ccaagctggt ataaaattta
     1861 ataacaaagg tttttggccg gcaggtgttc aaaatacact tcgaaatgct actccagcta
     1921 cagctgtaga gactacttat atctacaaag aaagttccaa gtatggtgat gtcattgttg
     1981 agtactacga tactgacgga aaacaaattg taaattcagt tgtagatact cctaagtcag
     2041 ctcttggcac agagtataat acagatgtgg accgtagacc agccagcttg gttgctgctg
     2101 atgggacagt ctacttctac aaagaagtta agtctgattc agctaagaca accggtacag
     2161 tagttgcagg tacgacaact gttaagtatg tttacgaaaa agctggtagc gttaatgtta
     2221 acttcgttga catcaatggt aaagtaatca aagctcctgt ttcagatgaa aaagatgcga
     2281 aacctggtta caattatgat accgacttgg atcagaaatt agcttccatc acttttgaag
     2341 gcaaggaata caaacttgtt cctgctggtg attatccggt tggtaaagtt ggcaagggaa
     2401 ataacttgat tgaagttggt aataatactg cgaaaggtat tgacccaaca acaggcaaaa
     2461 ttgaagccgg tgttaacaaa gaagttacct atgtctatag agcagtgaca ggttctgtag
     2521 ttgtaaatta caaagataca gaaggtaatg tgattaaaga tccagaaacg gatgtgtctg
     2581 atgcaccggt tggagatgct tatactacaa ctgacaagaa accaaacgaa atcatcacaa
     2641 aagatggatc acgctatgtt cttgttccat ctaagacaga tggtgaggaa aatggtaaag
     2701 ttatcgaagg aacaatcaca gtaacttatg tttaccagaa agttgcaaac tggattccag
     2761 agattccaaa tgtaccagaa acagaccgtc caaaagtacc ttacccattt gacccaacag
     2821 agccagacga gccaatcgat ccaacgacac caggaacaaa tggcgaggtt ccaaatattc
     2881 cttacgttcc aggatataca ccggttgatc ctaaggataa cacgccgttg aaaccaattg
     2941 atccaaatga tccaggtaag ggttatgtac caccaacacc agaaaatcca ggtgttgata
     3001 caccaattcc ttatgttcca gttaaaaaag tcgtaactaa ccacgttgat gaagagggta
     3061 accctattgc accgcaagaa gagggaacaa aaccaaacaa atcaatccca ggttacgagt
     3121 tcacaggtaa aactgttact gacgaagatg gcaacacaac tcacatctac aagaaaacac
     3181 cagaagttaa gaatggtaca gttgttgtta actatgtaac agaagatggc acagttatca
     3241 aggaacctgt aacagataca ccaacttctc cagaaggcac accatacgac actacagaca
     3301 acaaacctaa gacaatcact ttcaaaggtg aagagtatga attggttcgt gttgacggta
     3361 cagaaaacgg taaagttgta gaaggtgaaa cagttgtgac ttacgtttac cgtaaagtcg
     3421 aaacacctgc taagaaagtt gtaactaacc acgttgatga agagggtaac cctgttgcgc
     3481 cgcaagaaga gggaacaaaa ccaaacaaat caatcccagg ttacgaattt acaggtaaaa
     3541 ctgttactga cgaagatggc aacacaactc acatctacaa gaaaacacct gctaagaaag
     3601 ttgtgactaa ccacgttgat gaagaaggta accctattgc tccacaagag gatgggacaa
     3661 caccaaaacg tcaaatttca ggttacgagt atgtgcgtac tgtagttgat gaagaaggta
     3721 acacgacaca tatttatcgc aaactttcta ataaaccaac aacacctgag aaggaaactc
     3781 ctgcaaaacc tcaagcaggt aaaaccgctt caggtaaagc tcaattgcca aatactggtg
     3841 aggcttcatc tgtggcaggt gcgcttggta cagcaatgct tgtcgcaaca cttgcgtttg
     3901 caagaaaacg tcgtcgtaac gaagattagt caaaattctt tatacagact ttattccccc
     3961 acatagaaag tataagaatt gtacgtaaca tgcaggattg cctttccgaa aaaatgaggc
     4021 tgggcaaaaa gtccagagtt acatcttaga gttcgctcca tttccaacct ccaacagtca
     4081 ctactctgac tgttggagct gtgtgggggt gggagacgag c
//