From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Synthetic plasmid pDSP1: a cloning vector for eukaryotic genes and
Date: 24 Jul 92 10:10:14 GMT
Distribution: bionet
Checksum: 56360     7

LOCUS       SYNDSP1      2553 bp ds-DNA             SYN       24-JUL-1992
DEFINITION  Synthetic plasmid pDSP1: a cloning vector for eukaryotic genes and
            gene regulatory elements, segment 1.
ACCESSION   M12159
KEYWORDS    galK gene.
SEGMENT     1 of 2
SOURCE      SV40 DNA and E.coli DNA.
  ORGANISM  Cloning vector
            Artificial sequences; Cloning vehicles.
REFERENCE   1  (bases 382 to 409)
  AUTHORS   Reff,M.E., Sathe,G. and Pfarr,D.S.
  TITLE     Laboratory methods: A highly modular cloning vector for the
            analysis of eukaryotic genes and gene regulatory elements
  JOURNAL   DNA 4, 461-467 (1985)
  STANDARD  full staff_review
REFERENCE   2  (bases 1 to 2553)
  AUTHORS   Reff,M.E.
  JOURNAL   Unpublished (1986) Smith Kline and French Labs, Swedeland PA 19479
  STANDARD  full staff_review
COMMENT     Draft entry and sequence in computer readable form for [1] and [2]
            kindly provided by M.E.Reff, 29-MAY-1986.
            Vector pDSP1 contains two independent mammalian transcription
            cassettes containing SV40 early gene regulatory elements
            controlling either the E.coli galK or xgprt genes.  The regulatory
            elements of the galK cassette are bounded by multiple restriction
            sites allowing for easy removal and replacement of the regulatory
            sequences or the galK gene itself.
FEATURES             Location/Qualifiers
     misc_recomb     <1..1
                     /note="pBR322 DNA end/M13 DNA start"
     misc_recomb     38..39
                     /note="M13 DNA end/SV40 early promoter start"
     misc_recomb     384..385
                     /note="SV40 early promoter DNA end/synthetic linker start"
     CDS             395..1543
                     /partial
                     /note="galK"
                     /codon_start=1
                     /translation="MALQEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLDAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     misc_recomb     406..407
                     /note="synthetic linker end/galK cds start (AA 5)"
     misc_recomb     1589..1590
                     /note="galK DNA end/synthetic linker start"
     misc_recomb     1641..1642
                     /note="synthetic linker end/SV40 DNA sart"
     misc_recomb     1776..1777
                     /note="SV40 DNA end/synthetic linker start"
     misc_recomb     1800..1801
                     /note="synthetic linker end/SV40 late region DNA start"
BASE COUNT      653 a    666 c    601 g    633 t
ORIGIN      34 bp upstream of EcoRI site.
        1 agcttggctg caggtcgacg gatcgatccc cgggaattct gtggaatgtg tgtcagttag
       61 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
      121 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
      181 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
      241 ctccgcccag ttccgcccat tctccgcccc atgcatggct gactaatttt ttttatttat
      301 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
      361 ggaggcctag gcttttgcaa aaagcttggc caccatggct ctccaagaaa aaacacaatc
      421 tctgtttgcc aacgcatttg gctaccctgc cactcacacc attcaggcgc ctggccgcgt
      481 gaatttgatt ggtgaacaca ccgactacaa cgacggtttc gttctgccct gcgcgattga
      541 ttatcaaacc gtgatcagtt gtgcaccacg cgatgaccgt aaagttcgcg tgatggcagc
      601 cgattatgaa aatcagctcg acgagttttc cctcgatgcg cccattgtcg cacatgaaaa
      661 ctatcaatgg gctaactacg ttcgtggcgt ggtgaaacat ctgcaactgc gtaacaacag
      721 cttcggcggc gtggacatgg tgatcagcgg caatgtgccg cagggtgccg ggttaagttc
      781 ttccgcttca ctggaagtcg cggtcggaac cgtattgcag cagctttatc atctgccgct
      841 ggacggcgca caaatcgcgc ttaacggtca ggaagcagaa aaccagtttg taggctgtaa
      901 ctgcgggatc atggatcagc taatttccgc gctcggcaag aaagatcatg ccttgctgat
      961 cgattgccgc tcactgggga ccaaagcagt ttccatgccc aaaggtgtgg ctgtcgtcat
     1021 catcaacagt aacttcaaac gtaccctggt tggcagcgaa tacaacaccc gtcgtgaaca
     1081 gtgcgaaacc ggtgcgcgtt tcttccagca gccagccctg cgtgatgtca ccattgaaga
     1141 gttcaacgct gttgcgcatg aactggaccc gatcgtggca aaacgcgtgc gtcatatact
     1201 gactgaaaac gcccgcaccg ttgaagctgc cagcgcgctg gagcaaggcg acctgaaacg
     1261 tatgggcgag ttgatggcgg agtctcatgc ctctatgcgc gatgatttcg aaatcaccgt
     1321 gccgcaaatt gacactctgg tagaaatcgt caaagctgtg attggcgaca aaggtggcgt
     1381 acgcatgacc ggcggcggat ttggcggctg tatcgtcgcg ctgatcccgg aagagctggt
     1441 gcctgccgta cagcaagctg tcgctgaaca atatgaagca aaaacaggta ttaaagagac
     1501 tttttacgtt tgtaaaccat cacaaggagc aggacagtgc tgaacgaaac tcccgcactg
     1561 gcacccgatg gtcagccgta ccgactgttc aagctagaat tctctagagc tcgctgatca
     1621 gcctcgaggc cagctagctt gaacttgttt attgcagctt ataatggtta caaataaagc
     1681 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     1741 tccaaactca tcaatgtatc ttatcatgtc tggatccctc gaggggggat cccgtcgacc
     1801 gatccccagg aagctcctct gtgtcctcat aaaccctaac ctcctctact tgagaggaca
     1861 ttccaatcat aggctgccca tccaccctct gtgtcctcct gttaattagg tcacttaaca
     1921 aaaaggaaat tgggtagggg tttttcacag accgctttct aagggtaatt ttaaaatatc
     1981 tgggaagtcc cttccactgc tgtgttccag aagtgttggt aaacagccca caaatgtcaa
     2041 cagcagaaac atacaagctg tcagctttgc acaagggccc aacaccctgc tcatcaagaa
     2101 gcactgtggt tgctgtgtta gtaatgtgca aaacaggagg cacattttcc ccacctgtgt
     2161 aggttccaaa atatctagtg ttttcatttt tacttggatc aggaacccag cactccactg
     2221 gataagcatt atccttatcc aaaacagcct tgtggtcagt gttcatctgc tgactgtcaa
     2281 ctgtagcatt ttttggggtt acagtttgag caggatattt ggtcctgtag tttgctaaca
     2341 caccctgcag ctccaaaggt tccccaccaa cagcaaaaaa atgaaaattt gacccttgaa
     2401 tgggttttcc agcaccattt tcatgagttt tttgtgtccc tgaatgcaag tttaacatag
     2461 cagttacccc aataacctca gttttaacag taacagcttc ccacatcaaa atatttccac
     2521 aggttaagtc ctcatttaaa ttaggcaaag gaa
//