From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Theiler's murine encephalomyelitis virus 2A polyprotein encoding
Date: 29 Sep 92 10:10:29 GMT
Distribution: bionet
Checksum: 08720     9

LOCUS       TMEPPCOAT    2790 bp ss-RNA             VRL       29-SEP-1992
DEFINITION  Theiler's murine encephalomyelitis virus 2A polyprotein encoding
            leader protein and coat protein mRNA, complete cds.
ACCESSION   M94868
KEYWORDS    coat protein; structural protein.
SOURCE      Theiler's murine encephalomyelitis virus (strain Vilyuisk) cDNA to
            genomic RNA.
  ORGANISM  Theiler's murine encephalomyelitis virus
            Viridae; ss-RNA nonenveloped viruses; Isometric ss-RNA viruses;
            Picornaviridae; Enterovirus.
REFERENCE   1  (sites)
  AUTHORS   Pritchard,A.E., Calenoff,M.A., Simpson,S., Jensen,K. and
            Lipton,H.L.
  TITLE     A single base deletion in the 5' noncoding region of Theiler's
            virus attenuates neurovirulence
  JOURNAL   J. Virol. 66, 1951-1958 (1992)
  STANDARD  full staff_review
REFERENCE   2  (bases 1 to 2790)
  AUTHORS   Pritchard,A.E., Strom,T. and Lipton,H.L.
  TITLE     Nucleotide sequence identifies vilyuisk virus as a divergent
            Theiler's virus
  JOURNAL   Virology (1992) In press
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     CDS             1..2790
                     /product="polyprotein"
                     /evidence=EXPERIMENTAL
                     /codon_start=1
                     /translation="MACKHGYPDVCPICTAIDVTPGFEYLLLADGEWFPTDLLCVDLD
                     DDVFWPSDSSNQSQTMEWTDIPLICDTVMEPQGNSTSSDKSNSQSSGNEGVIINNFYS
                     NQYQNSIDLSANGGNAGDGPKTEGQLSNILGGAANAFATMAPLLLDENTEEMENLSDR
                     VDSDKAGNSATNTQSSVGRLHGYGATHRGDHPASCADTATDKVLAAERYYTIDLATWT
                     TAQTTFSHIRVPLPHALAGEHGGVFGATLRRHYLAKCGWRVQVQCNASQFHAGSLLVF
                     LAPEFYTGTGVATSGQEPNKVFLMDTTWQEPQAAPTGFRYDGKNGFFTLNHQNYWQWT
                     VYPHQILNLRTNTSVDLEVPYVNVAPTSSWTQHANWALVVAVLTPLQYSTGAATDVAI
                     TVSLQPVNPVFNGLRHEAQVPQSPVAVTVREHQGSFYSTNPDTTVPIYGKTIVTPSDY
                     MCGEFTDLLELCKLPTFLGNLSNDTRVPFFTATNSVPTESLVEYQVTLSCSCMSNSML
                     ASVARNFNQYRGSLNFLFVFTGSAMTKGKFLIAYTPPGAGKPTTRDQAXQSTYAIWDL
                     GLNSSYNFTVPFISPSHYRQTSYTSPSIAAVDGWLTVWQLTPLTFPANVPPSSDILTL
                     VSAGNDFTLRMPISPTKWIPQGVDNAEKGKVSDDNASVDFVAEPIKLPENQTRVNFFY
                     DRSSPIGLLRPNQAIESNFSYSADSNGATNCALLTPLPSYSPDRPGQSPDTSKAPIQW
                     RWISAVTESGTVSNTFPTRTRQDYAFLLFSPFTYYKCDLEVTLSSVGNGVVASLVRWA
                     PTGAPADITTQLTTSTPSIGDTRDPHMWLVGAGNSQTSFVIPYNSPLSVLPAAWFNGW
                     SNFSNTYDFGIAPCSDFGRLWIQGNAPLAIRVRYKKMRVFCPRPTLFFPWPTPTTTKV
                     NADNPVPILDLENPAA"
                     /citation=[2]
                     /citation=[1]
                     /partial
     mat_peptide     1..228
                     /product="leader peptide"
                     /codon_start=1
     CDS             14..484
                     /note="in vitro evidence for protein in DA strain of
                     virus"
                     /product="alternative translation site protein"
                     /note="putative"
                     /codon_start=1
                     /translation="MDTQTCALFAQPLTLLPALNICSWRTENGSQRTFFVWTWTMTSS
                     GLRTRAINLKQWNGLTYRSYAILSWNPRETPRHLTRVTPSPQEMKGLLLITSIPINTK
                     IQLICLPMEATLATVPRLKDNFPTSWAALLMPLLLWHLSSWMKTQRRWKISLTE"
                     /citation=[2]
     mat_peptide     229..441
                     /function="structure"
                     /standard_name="1A, VP4"
                     /product="coat protein"
                     /gene="1A"
                     /evidence=EXPERIMENTAL
                     /codon_start=1
                     /citation=[2]
     mat_peptide     442..1242
                     /function="structure"
                     /standard_name="1B, VP2"
                     /product="coat protein"
                     /gene="1B"
                     /evidence=EXPERIMENTAL
                     /codon_start=1
                     /citation=[2]
     mat_peptide     1243..1935
                     /function="structure"
                     /standard_name="1C, VP3"
                     /product="coat protein"
                     /gene="1C"
                     /evidence=EXPERIMENTAL
                     /codon_start=1
                     /citation=[2]
     mat_peptide     1936..2778
                     /function="structure"
                     /standard_name="1D, VP1"
                     /product="coat protein"
                     /gene="1D"
                     /evidence=EXPERIMENTAL
                     /codon_start=1
                     /citation=[2]
     mat_peptide     2779..>2790
                     /function="unknown"
                     /gene="2A"
                     /note="putative"
                     /codon_start=1
                     /citation=[2]
BASE COUNT      646 a    805 c    569 g    769 t      1 others
ORIGIN
        1 atggcctgca aacatggata cccagacgtg tgccctattt gcacagccat tgacgttact
       61 cccggctttg aatatttgct cttggcggac ggagaatggt tcccaacgga ccttctttgt
      121 gtggacttgg acgatgacgt cttctggcct tcggactcga gcaatcaatc tcaaacaatg
      181 gaatggactg acataccgct catatgcgat actgtcatgg aaccccaggg aaactccacg
      241 tcatctgaca agagtaactc ccagtcctca ggaaatgaag gggttattat taataacttc
      301 tattccaatc aataccaaaa ttcaattgat ttgtctgcca atggaggcaa cgctggcgac
      361 ggtcccaaga ctgaaggaca actttccaac atcttgggcg gcgctgctaa tgcctttgct
      421 actatggcac ctctcctcct ggatgaaaac acagaggaga tggaaaatct ctctgacaga
      481 gtagattctg acaaagctgg aaattcggcc acaaacactc aatcctccgt tggccggctc
      541 cacggctacg gtgcaactca ccgtggagac caccctgctt cctgtgctga cacagctact
      601 gataaagttc ttgccgctga acgctactac accattgatt tggctacttg gactacggca
      661 cagactactt tttcccacat cagagtgcct ctgccccacg ccctcgctgg cgagcacggt
      721 ggggtatttg gtgctaccct gagacggcat tatctcgcca agtgtggttg gcgcgtgcaa
      781 gttcaatgta atgcttccca attccacgcc ggctctcttc ttgttttcct ggctcctgaa
      841 ttctacactg gcactggtgt tgccactagt ggacaggaac ctaataaagt ctttctcatg
      901 gatactacat ggcaagaacc acaagctgcc cccactggtt ttcgctatga tggcaaaaac
      961 gggttcttta ctctcaatca tcaaaattat tggcaatgga ctgtctaccc gcatcaaatt
     1021 ctcaacttgc gcacaaatac gtctgttgat cttgaagttc cctatgtgaa tgttgctccc
     1081 acaagctcat ggacacaaca cgccaattgg gctttggtgg tggctgtgct cacccccctg
     1141 cagtattcga ccggtgcagc cactgatgta gcaattactg tttcccttca accagttaac
     1201 ccagttttca acggtcttcg acatgaggct caggttcccc aaagccccgt tgctgtcact
     1261 gttcgtgaac atcaaggcag cttctactcc acaaaccctg atacaactgt ccccatttat
     1321 ggtaagacta tagtcacacc cagtgactac atgtgtggag aattcactga ccttcttgag
     1381 ctgtgtaaat tacccacttt tcttggaaat ttgagtaacg acactcgtgt tcccttcttc
     1441 actgccacca attccgtacc tacggaatcc ctagttgaat accaagttac actttcttgc
     1501 tcctgtatgt ccaactctat gcttgcctct gtggctcgta acttcaatca ataccgtggc
     1561 tccctcaact ttctttttgt ctttacaggt tctgccatga cgaaagggaa gttcttgatt
     1621 gcctacacgc cgcctggcgc tggcaagccc accactcgtg accaggctnt gcaatccact
     1681 tacgccatct gggatcttgg tctgaattcg agctacaatt ttactgtgcc tttcatttcg
     1741 ccttcccact accgtcaaac gagctacacc agtccgtcca tcgcggcggt tgacggatgg
     1801 ctcactgtct ggcagcttac tcctctcacc ttccccgcta atgtgccgcc tagctctgac
     1861 attctcactc tcgtttctgc ggggaacgat ttcaccttaa gaatgccgat ttctcccact
     1921 aaatggatcc cccaaggtgt ggataatgct gaaaaaggaa aagtgtctga tgataatgct
     1981 tctgttgact ttgttgccga acctattaaa ttgcctgaga atcaaacgcg agttaatttc
     2041 ttctatgacc gttcctcacc aattggcctt ctacgaccta atcaggcaat agaaagcaat
     2101 ttctcctact ctgctgattc taatggtgcc actaattgtg ctctccttac ccccctacca
     2161 tcttacagcc cagatagacc gggtcagagt cctgacacta gtaaagctcc catacaatgg
     2221 cgctggataa gtgccgtcac cgagtctggt actgtgtcta acacctttcc caccagaact
     2281 agacaagact atgccttcct gctcttttcc cctttcacct actacaagtg tgacctggaa
     2341 gtgactctta gttccgtggg taatggtgtt gttgcctctc ttgtgcgctg ggctcctact
     2401 ggtgccccag ctgacataac cactcaactt accacatcca cgcctagtat tggtgacacc
     2461 cgcgatcccc acatgtggct ggtgggtgcg ggcaactccc agacatcttt tgtcataccc
     2521 tacaactccc ccctgtccgt cttgcctgcc gcctggttca atggatggtc taacttttcc
     2581 aacacgtacg attttggaat tgctccctgt tctgactttg gacggctctg gatacaaggc
     2641 aatgctccac ttgctattcg cgtacgctat aagaaaatga gagtcttttg tcccagaccc
     2701 acacttttct tcccctggcc tacccccact acaacaaaag ttaatgccga taatccagtt
     2761 cctatccttg acctcgagaa tcctgctgca
//