From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: E.caballus mRNA for pancreatic lipase
Date: 4 Sep 92 10:03:23 GMT
Distribution: bionet
Checksum: 07414     5

LOCUS       HRSPL        1443 bp ss-RNA             MAM       04-SEP-1992
DEFINITION  E.caballus mRNA for pancreatic lipase
ACCESSION   X66218
KEYWORDS    lipolytic enzyme; pancreatic lipase.
SOURCE      Equus caballus RNA.
  ORGANISM  Equus caballus
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Perissodactyla; Equidae.
REFERENCE   1  (bases 1 to 1443)
  AUTHORS   Kerfelec,B.
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
REFERENCE   2  (sites)
  AUTHORS   Kerfelec,B., Foglizzo,E., Bonicel,J., Bougis,P.E. and Chapus,C.
  TITLE     Sequence of horse pancreatic lipase as determined by protein and
            cDNA sequencing: Implications for p-nitrophenyl acetate hydrolysis
            by pancreatic lipases
  JOURNAL   Eur. J. Biochem. 206, 279-287 (1992)
  STANDARD  full staff_review
COMMENT     *source: tissue=pancreas; *source: clone_library=pancreatic cDNA;
            From EMBL    entry ECPL;  dated 11-AUG-1992.
FEATURES             Location/Qualifiers
     CDS             <1..1386
                     /product="pancreatic lipase"
                     /gene="lipase"
                     /codon_start=1
                     /translation="WTLSLLLGAVVGNEVCYERLGCFSDDSPWAGIVERPLKILPWSP
                     EKVNTRFLLYTNENPDNFQEIVADPSTIQSSNFNTGRKTRFIIHGFIDKGEESWLSTM
                     CQNMFKVESVNCICVDWKSGSRTAYSQASQNVRIVGAEVAYLVGVLQSSFDYSPSNVH
                     IIGHSLGSHAAGEAGRRTNGAVGRITGLDPAEPCFQGTPELVRLDPSDAQFVDVIHTD
                     IAPFIPNLGFGMSQTAGHLDFFPNGGKEMPGCQKNVLSQIVDIDGIWQGTRDFAACNH
                     LRSYKYYTDSILNPDGFAGFSCASYSDFTANKCFPCSSEGCPQMGHYADRFPGRTKGV
                     GQLFYLNTGDASNFARWRYRVDVTLSGKKVTGHVLVSLFGNKGNSRQYEIFQGTLKPD
                     NTYSNEFDSDVEVGDLEKVKFIWYNNVINLTLPKVGASKITVERNDGSVFNFCSEETV
                     REDVLLTLTAC"
     sig_peptide     <1..36
                     /gene="lipase"
     mat_peptide     37..1386
                     /product="pancreatic lipase"
                     /gene="lipase"
                     /evidence=EXPERIMENTAL
     misc_feature    37..1044
                     /note="N-terminal domain"
                     /evidence=EXPERIMENTAL
     misc_feature    748..822
                     /note="flap"
     misc_feature    1045..1356
                     /note="C-terminal domain"
                     /evidence=EXPERIMENTAL
     polyA_signal    1422..1428
BASE COUNT      394 a    324 c    356 g    369 t
ORIGIN
        1 tggacacttt cgctgctgct gggagcagta gtaggaaacg aagtttgcta tgagagactc
       61 ggctgcttca gtgacgattc gccgtgggcc gggattgtgg aaagacctct caaaatattg
      121 ccctggtctc cagaaaaggt caacacccgc ttcctcctgt acactaacga gaacccagac
      181 aactttcaag aaatcgttgc agatccttca actatccaga gctccaattt caacacaggt
      241 agaaaaaccc gctttattat tcatggattc atagacaagg gagaagaaag ctggctgtcc
      301 actatgtgcc agaacatgtt taaggtggaa agtgtgaact gtatctgtgt ggactggaaa
      361 agtggctccc gcaccgcata ctcacaggct tcacagaacg tccggattgt gggggcagaa
      421 gtggcatatc ttgttggagt tcttcagtca tcattcgact actctccttc caacgtccac
      481 atcattggcc atagcctggg ttctcacgct gctggggagg ctggaaggag gaccaatggg
      541 gccgttggac ggatcacagg gttggatcca gctgaacctt gctttcaggg cacacccgaa
      601 ttagtccgat tggaccccag cgatgcccaa tttgtggatg taattcacac agacattgcc
      661 cccttcatcc ccaacttggg gtttggaatg agccaaactg caggccacct ggatttcttt
      721 ccaaatggag gaaaagaaat gcccggatgt caaaagaacg ttctctctca gattgttgac
      781 atagacggga tctggcaagg aactcgtgac tttgcggcct gtaatcactt aagaagctac
      841 aagtattaca ctgatagcat cctcaaccct gatggctttg ctggattctc ttgtgcctct
      901 tacagtgatt tcactgcaaa caagtgcttc ccctgttcaa gtgaaggctg cccacagatg
      961 ggtcattatg ctgatagatt tcctgggaga acaaaaggag tgggccagct attttatcta
     1021 aacaccggtg atgccagcaa ttttgcccgt tggaggtata gggtagatgt cacactgtct
     1081 ggaaagaagg ttacaggaca cgtgctagtt tctttgtttg gaaataaagg gaactctagg
     1141 cagtatgaaa ttttccaggg cactctcaaa ccagacaata cttattccaa tgaatttgac
     1201 tcagatgtgg aagttggaga tttggagaag gttaaattta tttggtacaa caatgtgatc
     1261 aacctaactc tacccaaagt aggagcatcc aagatcacag tggaaagaaa cgatggaagt
     1321 gtgttcaact tctgtagtga agaaaccgtg agggaagatg ttctgctcac tctcactgcg
     1381 tgttaggagc tgctgacatg tgaccattga atctccctgc taataaaatc tactggtgaa
     1441 gca
//