From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: lactose operon: orf X 5' of
Date: 29 Dec 92 19:59:53 GMT
Distribution: bionet
Checksum: 64566 13

LOCUS       S115188      5140 bp    DNA             NLM       10-DEC-1992
DEFINITION  lactose operon: orf X 5' of
            lacR...lacE=phosphoenolpyruvate-dependent PTS transport component
            EII Lac [Streptococcus mutans, Genomic, 8 genes, 5140 nt]
ACCESSION   S45749 M80797
KEYWORDS    .
SOURCE      Streptococcus mutans
  ORGANISM  Streptococcus mutans
            Unclassified.
REFERENCE   1  (bases 1 to 5140)
  AUTHORS   Rosey,E.L. and Stewart,G.C.
  TITLE     Nucleotide and deduced amino acid sequences of the lacR, lacABCD,
            and lacFE genes encoding the repressor, tagatose 6-phosphate gene
            cluster, and sugar-specific phosphotransferase system components of
            the lactose operon of Streptococcus mutans.
  JOURNAL   J. Bacteriol. 174, 6159-6170 (1992)
  STANDARD  full automatic
COMMENT     This entry [NCBI gibbsq 115188] was created by the journal scanning
            component of NCBI/GenBank at the National Library of Medicine.
            This sequence comes from Fig. 3.
            Region: lactose operon.  
FEATURES             Location/Qualifiers
     CDS             2..247
                     /partial
                     /note="Description: orf X 5' of lacR;  For the protein
                     sequence (NCBI gibbsq 115191):  Method: conceptual
                     translation supplied by author.  This sequence comes from
                     Fig. 3."
                     /codon_start=1
                     /translation="DPASGEDYVVRGIIDGYILFDNRIVLFDYKTDKFTNSQAIKERY
                     RGQMTLYAQALSQSYNIQQVDSYLILLGGEKLEVVEI"
     CDS             472..1227
                     /note="lactose operon repressor homolog;  For the protein
                     sequence (NCBI gibbsq 115193):  Method: conceptual
                     translation supplied by author.  This sequence comes from
                     Fig. 3."
                     /gene="lacR"
                     /product="LacR"
                     /codon_start=1
                     /translation="MKKEERLEEITKLINKRGTIRVTEVVERLKVSDMTVRRDLTELE
                     GLGVLTRIHGGARSNNIFQYKEMSHEEKHSRQIEEKHYIAQKAAELVEEGDTIFLGPG
                     TTVELLAEEINKTTLQVITNCLPVFQILSQKQSETFRVHLLGGEMRSITQSFIGEITN
                     IVLEKMHFSKMFFSGNGVKGNEVMTSSFQEAYTQKMALGRAIEKYFLIDSSKIGKEDF
                     TSFYQLSQLTALITDCQDDDKLQKLSKYTEIIN"
     CDS             1534..1962
                     /note="LacA;  For the protein sequence (NCBI gibbsq
                     115194):  Method: conceptual translation supplied by
                     author.  This sequence comes from Fig. 3."
                     /gene="lacA"
                     /product="galactose-6-phosphate isomerase subunit"
                     /codon_start=1
                     /translation="MAIIIGSDAAGKRLKDVIKTFLKDNNHEVLDVTERKDLDFVDST
                     LAVVHEVQKNDKNLGIAIDAYGAGSFMVATKVKGMIAAEVSDERSAYMTRGHNNARII
                     TLGAEIVGDELAKNIVKDFVEAKYDGGRHQIRVDMLNKMC"
     CDS             1988..2503
                     /note="LacB;  For the protein sequence (NCBI gibbsq
                     115199):  Method: conceptual translation supplied by
                     author.  This sequence comes from Fig. 3."
                     /gene="lacB"
                     /product="galactose-6-phosphate isomerase subunit"
                     /codon_start=1
                     /translation="MKIAIGCDHIVTDVKMELSKHLKEEGYEVLDVGTYDFTRTHYPI
                     FGKKVGEAVSSGEADLGVCMCGTGVGISNAANKVPGVRTALVRDMTSALYSKEELNAN
                     VVSFGGAIIGKLLLFDIVDAFIKAQYKPTEENKKLIAKIKHLEAHNDKQADPHFFDEF
                     LEKWNRGDYHD"
     CDS             2522..3454
                     /note="tagatose-6-phosphate kinase homolog;  For the
                     protein sequence (NCBI gibbsq 115200):  Method: conceptual
                     translation supplied by author.  This sequence comes from
                     Fig. 3."
                     /gene="lacC"
                     /product="LacC"
                     /codon_start=1
                     /translation="MMLTVTMNPSIDIAYQLDDLKVDTVNRVIETHKTPGGKGLNVTR
                     VLSQLGDDVLASGLLGGKLGEFLEAELDKSAIKHSFYKISAETRNCIAILHGGYQTEI
                     LEQGPYVSAKESKGFLEFFEKLLPKLEVVAISGSLPKGVPVDYYSQMIAICKQHQVPI
                     VLDCSGQALLEVLNGAAKPTVIKPNTEELSQIMEREITNDVAVLKHALASPIFSGIDW
                     IIVSLGSQGAFAKHGQTFYKVTIPKIAVVNPVGSGDSTVAGITSALAAGASDEKLLKK
                     ANTLGMLNAQEKLTGHVNLENYDNLYQQIEVAEV"
     CDS             3459..4436
                     /note="tagatose-1,6-bisphosphate aldolase homolog;  For
                     the protein sequence (NCBI gibbsq 115203):  Method:
                     conceptual translation supplied by author.  This sequence
                     comes from Fig. 3."
                     /gene="lacD"
                     /product="LacD"
                     /codon_start=1
                     /translation="MTLTQEKRSYMEKLSDENGIISALAFDQRGALKRLMAQYQTQEP
                     TIAQMEELKVLVAEELTPYASSMLLDPEYGLPAAKHLDKNAGLLLAYEKTGYDTTSTK
                     RLPDCLVEWSAKRLKKQGADAVKFLLYYDVDGDEEVNQQKQAYIERIGSECKAEDIPF
                     FLEILAYDETITDAASVEYAKVKPHKVLDAMKVFSDERFGIDVLKVEVPVNMKYVEGF
                     GDGPIVHTQDQAANFFKQQDQATPLPYIYLSAGVSAKLFQDTLVFAKESGANFNGVLC
                     GRATWAGSVKDYIEKGEAAARQWLRTEGFKNIDELNKVLKATATSWEER"
     CDS             4452..4766
                     /note="LacF;  For the protein sequence (NCBI gibbsq
                     115206):  Method: conceptual translation supplied by
                     author.  This sequence comes from Fig. 3."
                     /gene="lacF"
                     /product="phosphoenolpyruvate-dependent PTS transport
                     component EIII Lac"
                     /codon_start=1
                     /translation="MNREEATLLGFEIVAYAGDARSKLLEALNAAQAGEYDRAEELVA
                     AADDCIVDAHKAQTSLLAKEAQGDDIELSVTLMHGQDHLMTTILLKDLMKHLIELYKR
                     GS"
     CDS             4773..5140
                     /partial
                     /note="LacE;  For the protein sequence (NCBI gibbsq
                     115208):  Method: conceptual translation supplied by
                     author.  This sequence comes from Fig. 3."
                     /gene="lacE"
                     /product="phosphoenolpyruvate-dependent PTS transport
                     component EII Lac"
                     /codon_start=1
                     /translation="MNTLIAQIEKGKPFFEKISRNIYLRAIRDGFISAMPVILFSSIF
                     LLIAYVPNIFGFTWPKGIENMLMTPYNYTMGIIGFLVAGTTAKSLTDSMNRQLEKTNQ
                     INFISTMLASMAGFLIMAADP"
BASE COUNT     1745 a    787 c   1094 g   1514 t
ORIGIN      
        1 ggatcctgca agcggagaag actatgtcgt gcgggggatt attgacggtt acattctttt
       61 tgataatcgg attgtcctct ttgattataa aacagataaa ttcactaata gtcaagctat
      121 taaagagcgt taccgaggtc agatgacact ttatgctcaa gctttgagcc aatcctataa
      181 tatccaacaa gtagacagtt atttaattct attaggcggt gaaaaattag aagttgtgga
      241 aatttaatag caaacatatt ccaaaaaaga gtagtctaat gaaaagaacc acttataatg
      301 gacagtataa aaaagttatg cactgttttt ataaggaggt tcttttcata aatactcttt
      361 tttatttatg aaaagaactg ttatttaaac gtaatgtttg ttttatgttt gaatatcgaa
      421 aaaaatttaa aaatattata taatagaaga aaagaaggaa aagaggtatt tatgaagaag
      481 gaagaacgat tagaagaaat cacaaaactc attaataagc gagggaccat tcgggttact
      541 gaagttgtgg aacgattaaa ggtatcagac atgactgtcc gtcgcgattt aactgaattg
      601 gaaggcttgg gagtattaac acgtattcac ggtggggcta gaagcaataa tatttttcaa
      661 tataaagaaa tgtcccatga agaaaagcat tctcgacaaa tagaagaaaa gcactatatt
      721 gcacaaaagg ctgccgaatt agtagaagaa ggagatacga tctttttagg accaggaaca
      781 actgtagaac tgctggctga agaaattaat aagacaactt tgcaagtcat caccaattgt
      841 cttcctgttt ttcagatctt atcgcaaaaa caatcagaga cgtttagagt ccatttattg
      901 ggcggcgaaa tgagaagtat cactcagtct tttattggag aaataacaaa tatcgtttta
      961 gaaaaaatgc atttttctaa aatgtttttc agcggcaatg gtgtcaaagg aaatgaagtg
     1021 atgacatcaa gtttccaaga agcttatact cagaagatgg ctttaggaag ggcaattgaa
     1081 aaatatttcc tgattgactc ttcaaagatt ggaaaagaag attttacgtc attttatcag
     1141 ctatcacaat tgacagctct gattacagat tgtcaagatg acgataagct tcaaaaactg
     1201 agcaaatata ctgagattat taactaaatc gttagataac aaaaagtgat cagaaaaata
     1261 agataatcaa agagagcgag agaagacctt caagattgtt tagcaatcat agcggtctga
     1321 ctcggactct tatctttttg cgtaaaaaca ctcaaacttc aaacaaaaat tatattttat
     1381 caacagaaaa aggaaatatt ttttaaataa taccaaaaat gtttaaaaaa taaacaaaaa
     1441 tgtgttgatt ttttaaacaa gttatagtgt attataacta aagaaaaacg aaaacgcttt
     1501 tctgactaaa aaatcatttc aaggagaatg actatggcaa ttattattgg ttcagatgca
     1561 gctggtaaaa gacttaaaga tgttattaaa acatttttaa aagacaacaa tcatgaagtt
     1621 ttagatgtta ctgaaaggaa agatttggat tttgtagatt caactttagc agttgtacat
     1681 gaagtacaaa aaaatgataa aaatttgggg attgcaattg atgcctatgg tgcgggcagc
     1741 tttatggtag ctactaaagt caaaggtatg attgcagcag aagtgtctga tgaacgttca
     1801 gcttatatga cacgcggcca taataatgcg cgtatcatta ctcttggtgc tgaaattgtt
     1861 ggagatgaac tggccaaaaa tattgttaag gactttgtag aggctaaata tgacggcggc
     1921 cgtcatcaga tccgtgtaga tatgctaaat aaaatgtgct aaagacaaat agagtgagga
     1981 aatgaaaatg aaaattgcta ttggttgtga ccatattgtt actgatgtga aaatggaact
     2041 atctaaacat ctaaaagaag aaggttatga agttctagat gtagggacct atgactttac
     2101 acgtacgcac tatcccatct ttggtaaaaa agtgggcgaa gcagttagca gtggtgaagc
     2161 ggatttaggt gtatgtatgt gcggtactgg tgtgggtata agtaatgctg ccaataaagt
     2221 accgggtgtt agaacggcat tagtaagaga tatgacttct gcactgtatt caaaagaaga
     2281 acttaatgca aatgtcgtta gctttggcgg cgctattata ggtaagttgt tattatttga
     2341 tattgtagat gcttttatta aagcccagta taaaccgaca gaagaaaata agaaattaat
     2401 tgctaagatc aaacatttag aagcacataa cgataaacaa gcagatccgc atttctttga
     2461 tgaatttcta gaaaagtgga atcgcggtga ctatcacgat tgagtaaggg gagttatcat
     2521 tatgatgcta acagttacga tgaatccttc cattgatatt gcttatcagc tggatgactt
     2581 aaaggttgat actgtcaatc gtgtcattga aacgcataaa acacccggag gaaaaggatt
     2641 gaatgtgaca cgtgttctgt ctcagttagg agacgatgtc cttgctagcg gccttcttgg
     2701 tgggaaactt ggtgaatttt tagaagcgga acttgataag tctgccataa agcattcttt
     2761 ttataagatt tctgcagaga caagaaattg tattgctatt ttacatggtg gctatcaaac
     2821 agaaatatta gaacaaggac cttatgtttc cgctaaagaa tctaaagggt ttcttgaatt
     2881 ttttgaaaaa ttacttccaa aattagaagt tgtcgcaatt tcaggaagtc ttccaaaagg
     2941 ggttcctgta gactattatt ctcaaatgat tgcgatttgc aagcaacatc aggttcctat
     3001 tgttttggat tgttcaggtc aggctttgtt ggaagtgtta aacggtgcag ctaaaccaac
     3061 tgtcatcaag cccaatacag aagaattatc tcaaattatg gaacgggaga ttacaaatga
     3121 tgttgctgtt ttaaagcatg ctttggctag ccctatcttt tcaggaattg attggattat
     3181 tgtctcactt ggttctcaag gtgcttttgc caagcatggt caaacatttt ataaggtcac
     3241 tattcctaaa atagcagtcg ttaatccagt tggttcaggg gattcaacgg tagctggaat
     3301 tacatcggct cttgctgcag gagcaagtga tgagaaattg ctcaaaaaag cgaatacact
     3361 tggtatgctc aatgctcagg aaaaattaac tggacacgtt aacttagaaa attatgataa
     3421 tttataccaa caaattgagg tagcggaggt ttaaaaagat gacattaaca caagaaaagc
     3481 gcagttatat ggaaaaactt agtgatgaaa atggaattat ttcagcttta gcttttgacc
     3541 agcgtggtgc tttaaaacgc ttgatggcgc agtatcaaac gcaagaacca acgattgctc
     3601 aaatggaaga gctgaaggtc ttagtagcag aagaattaac accttatgct tcatccatgc
     3661 tgcttgatcc agaatatggt cttccagcag caaaacattt ggataaaaat gcaggtttgc
     3721 tccttgctta tgagaagact ggttatgata caacaagcac taaacgcttg ccagattgtc
     3781 tggttgaatg gtcagccaaa cgtttgaaaa aacaaggtgc agatgctgtt aaattcttgc
     3841 tctactatga tgttgatggt gatgaagaag ttaaccagca aaaacaggct tatattgaac
     3901 gaattggttc tgaatgtaag gcagaagata ttcccttttt ccttgaaatt ctagcttatg
     3961 acgaaaccat tactgatgcc gcaagcgttg agtatgctaa agtaaagcct cataaagtgc
     4021 ttgatgctat gaaagtcttc tcagacgagc gttttggcat tgatgttctg aaggttgagg
     4081 ttcctgttaa catgaagtat gtcgaaggtt ttggtgatgg cccaattgtt cacactcaag
     4141 atcaagcagc aaacttcttt aaacaacaag atcaagcaac accgcttccc tatatttatt
     4201 tgagtgcagg tgtttctgct aagttattcc aagatacgct tgtttttgca aaagagtcag
     4261 gggctaattt taacggtgtc ctttgcggac gtgccacatg ggctggttca gttaaagatt
     4321 atattgaaaa aggcgaagca gcagcccgcc agtggcttcg tacagaagga ttcaagaata
     4381 ttgatgagct gaataaggtt ttaaaagcaa ccgcaacgtc ttgggaagaa agatagaaag
     4441 gtggaaaaga gatgaacaga gaagaagcga ctcttttggg gtttgaaatt gtagcctatg
     4501 caggagatgc acgttctaaa ttacttgaag ctttgaatgc ggcacaggcc ggtgagtacg
     4561 atagggcaga agagcttgta gctgcggcag atgactgtat tgttgatgcc cataaagctc
     4621 aaacaagcct tttggctaag gaagcacaag gcgatgatat tgaacttagt gttactttaa
     4681 tgcacggaca agatcacctg atgacaacca tacttctaaa agatttgatg aaacatttaa
     4741 ttgaattgta caaaagaggg agctaaaata atatgaacac attgattgct caaattgaaa
     4801 agggaaagcc tttctttgaa aaaatttcgc gtaatattta tttacgtgct attcgtgacg
     4861 ggtttatctc ggcaatgccg gtcatcctat tttcgagtat ttttctgttg atcgcttatg
     4921 ttcctaatat ctttggtttt acttggccaa aaggtattga aaacatgttg atgacaccct
     4981 ataactatac gatggggatt attggtttcc tagtggctgg tacaactgct aaatcactaa
     5041 cggattcgat gaaccgccaa ttagaaaaga ccaaccagat taatttcatt tcaacaatgt
     5101 tagcttctat ggccggtttc ttaatcatgg cagcggatcc
//