From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: C.elegans unc-33 mRNA
Date: 15 Aug 92 10:01:22 GMT
Distribution: bionet
Checksum: 53496    11

LOCUS       CELUNC33MR   3660 bp ss-RNA             INV       15-AUG-1992
DEFINITION  C.elegans unc-33 mRNA
ACCESSION   Z14146
KEYWORDS    unc-33 gene.
SOURCE      Caenorhabditis elegans RNA.
  ORGANISM  Caenorhabditis elegans
            Eukaryota; Animalia; Eumetazoa; Nematoda; Secernentea; Rhabditida;
            Rhabditina; Rhabditoidea; Rhabditidae.
REFERENCE   1  (bases 1 to 3660)
  AUTHORS   Shaw,J.E.
  JOURNAL   Unpublished (1992)
  STANDARD  full staff_review
REFERENCE   2  (bases 1 to 3660)
  AUTHORS   Li,W., Herman,R.K. and Shaw,J.E.
  TITLE     Analysis of the C. elegans Axonal Guidance and Outgrowth Gene
            unc-33
  JOURNAL   Genetics (1992) In press
  STANDARD  full staff_review
COMMENT     *source: strain=Bristol; *source: stage=early embryos; *source:
            clone_library=cDNA from library of J. Ahringer and J. *source:
            Kimble; *source: clone=pEH#1-1, pEH#12-1; *source: chromosome=IV;
            From EMBL    entry CEUNC33MR;  dated 03-AUG-1992.
FEATURES             Location/Qualifiers
     mRNA            1..3660
                     /note="longest of threemRNAs from unc-33 gene,3.8 kb.
                     message begins withtrans-spliced leader SL1."
                     /evidence=EXPERIMENTAL
                     /citation=[2]
                     /citation=[1]
     5'UTR           1..65
     CDS             66..2630
                     /note="largest of three putativepolypeptides encoded by
                     unc-33 gene"
                     /gene="unc-33"
                     /codon_start=1
                     /translation="MFPFLAPIRSANHVEQQGTKYYVEITFLADSPYSRAPSSTGTEK
                     SVIPTLRNLDDLETVSNKSRSSEGVNKLGSRPNSRSSAIVKKTSVSSLPTSARSEGKS
                     SPIPVKDAIPAPARHKNKGLEMSSAMMELFGGGGSTSPAPSKRENPADAPSDRVVSNA
                     KLSQPGPEWFEGFEQMDMTDIELPPDPNCPAEKVLRGEKSTPDFDSDWQEAKEDVLDP
                     QSYPKSFNPAESLPGPDIGAGVDDEEEPEAEAQEMEEPQYESKVDEKDDDDNGSSSSK
                     KGHPSEDGDSTRNGETPTDRRNSGAIEETADGESSAQSAAEKKNSGDDGNGGGGEMSI
                     LLVKNAQIVNDDAIFVADILIEDGIIQNVAPNLEAPEGAEVLDAAGKLALPAGIDVYT
                     QVTDSSVDDLSTGCKSAIAGGTGTIVEVVRPRGAESVVSAVKRVKNQLEKSGISCHVA
                     LSVAITDFCEQEMSELVKNEGINSFVLDGVSLTDDKLLELFEHVKRLGALIRVVPENK
                     SIVAMLEKKMLKLGVTGPEGFPQSRPESLEADRVSGVCVLGNLASCPISIVQVSSADS
                     LAAIEKARASGALAHAEIASAAVTADGSALFSQDLRFASAHLTDVPLRRGAPDRMIGA
                     LSTQPLVVCTSGHRPVNSATRVAAKDFAIAQKGSTGAEERMAVVWERAVRSGRIDAMR
                     FVAVTSTNAAKMFNMYPKKGRIAVGADADLVIWDASGKRVLESSRAQSSQENSMYDGL
                     TVHSVVTATIVGGKIAYQNGEVREAPVAGGFLRLSPNSPYLFSMVGQRDKFANVERVE
                     REASSQQQKPQQNGHHKNSGDFDRNRTKVMESSIDFGGSAANRPRNPPGGRTTGFW"
     mRNA            519..3660
                     /note="middle-sized mRNA(3.3 kb) of three encoded by
                     unc-33 gene. messagebegins with trans-spliced leader SL1."
                     /evidence=EXPERIMENTAL
     CDS             591..2630
                     /note="middle-sized of three putative polypeptidesencoded
                     by the unc-33 gene."
                     /product="hypothetical"
                     /gene="unc-33"
                     /codon_start=1
                     /translation="MDMTDIELPPDPNCPAEKVLRGEKSTPDFDSDWQEAKEDVLDPQ
                     SYPKSFNPAESLPGPDIGAGVDDEEEPEAEAQEMEEPQYESKVDEKDDDDNGSSSSKK
                     GHPSEDGDSTRNGETPTDRRNSGAIEETADGESSAQSAAEKKNSGDDGNGGGGEMSIL
                     LVKNAQIVNDDAIFVADILIEDGIIQNVAPNLEAPEGAEVLDAAGKLALPAGIDVYTQ
                     VTDSSVDDLSTGCKSAIAGGTGTIVEVVRPRGAESVVSAVKRVKNQLEKSGISCHVAL
                     SVAITDFCEQEMSELVKNEGINSFVLDGVSLTDDKLLELFEHVKRLGALIRVVPENKS
                     IVAMLEKKMLKLGVTGPEGFPQSRPESLEADRVSGVCVLGNLASCPISIVQVSSADSL
                     AAIEKARASGALAHAEIASAAVTADGSALFSQDLRFASAHLTDVPLRRGAPDRMIGAL
                     STQPLVVCTSGHRPVNSATRVAAKDFAIAQKGSTGAEERMAVVWERAVRSGRIDAMRF
                     VAVTSTNAAKMFNMYPKKGRIAVGADADLVIWDASGKRVLESSRAQSSQENSMYDGLT
                     VHSVVTATIVGGKIAYQNGEVREAPVAGGFLRLSPNSPYLFSMVGQRDKFANVERVER
                     EASSQQQKPQQNGHHKNSGDFDRNRTKVMESSIDFGGSAANRPRNPPGGRTTGFW"
     mRNA            1026..3660
                     /note="smallest (2.8 kb)of three messages encoded by
                     unc-33 gene."
                     /evidence=EXPERIMENTAL
     CDS             1059..2630
                     /note="smallest of three putative polypeptides encodedby
                     the unc-33 gene."
                     /product="hypothetical"
                     /gene="unc-33"
                     /codon_start=1
                     /translation="MSILLVKNAQIVNDDAIFVADILIEDGIIQNVAPNLEAPEGAEV
                     LDAAGKLALPAGIDVYTQVTDSSVDDLSTGCKSAIAGGTGTIVEVVRPRGAESVVSAV
                     KRVKNQLEKSGISCHVALSVAITDFCEQEMSELVKNEGINSFVLDGVSLTDDKLLELF
                     EHVKRLGALIRVVPENKSIVAMLEKKMLKLGVTGPEGFPQSRPESLEADRVSGVCVLG
                     NLASCPISIVQVSSADSLAAIEKARASGALAHAEIASAAVTADGSALFSQDLRFASAH
                     LTDVPLRRGAPDRMIGALSTQPLVVCTSGHRPVNSATRVAAKDFAIAQKGSTGAEERM
                     AVVWERAVRSGRIDAMRFVAVTSTNAAKMFNMYPKKGRIAVGADADLVIWDASGKRVL
                     ESSRAQSSQENSMYDGLTVHSVVTATIVGGKIAYQNGEVREAPVAGGFLRLSPNSPYL
                     FSMVGQRDKFANVERVEREASSQQQKPQQNGHHKNSGDFDRNRTKVMESSIDFGGSAA
                     NRPRNPPGGRTTGFW"
     3'UTR           2631..3660
                     /note="possible stem-loop structure in 3'UTR; 42bp
                     near-perfect inverted repeat at positions 2945to 2986 and
                     3015 to 3056."
     repeat_unit     2945..2986
                     /rpt_type=inverted
     repeat_unit     3015..3056
                     /rpt_type=inverted
BASE COUNT     1023 a    825 c    895 g    917 t
ORIGIN
        1 aaagcgcgca aaatacacat ttatcactag aggagggcca aatagttggc ggacatcact
       61 ggaaaatgtt cccgttttta gcaccaattc gatcagcaaa tcatgtagag caacaaggca
      121 caaagtacta cgtagaaatc acatttctag cagattctcc atattctaga gctccatcga
      181 gcactggcac cgagaaatct gtaattccaa cgctcaggaa tctggatgac ctggagactg
      241 tttctaataa aagtaggagt agtgaaggag tgaataagct agggagccgt ccgaacagcc
      301 gaagctcggc aattgttaaa aagacgagtg tgtcctcact gccaacgtcg gcccgatccg
      361 aaggaaaatc ctctccgatt ccggttaaag atgcgattcc ggctccagcg cggcacaaga
      421 acaagggtct cgagatgtcg tcggcaatga tggagctatt tggtggcgga ggcagcacgt
      481 caccagcgcc atcaaaacga gaaaacccag ccgacgcgcc atccgatcgt gttgtaagca
      541 atgctaaact ctctcagcct ggcccagaat ggtttgaagg gttcgaacag atggatatga
      601 cggatattga actacccccc gacccgaact gcccggcgga aaaggtgttg cgcggagaaa
      661 aatcgacgcc agacttcgat tcggattggc aagaagcgaa agaagacgtt ttagatcctc
      721 aaagctaccc gaaaagcttc aaccccgccg aatcgcttcc cggtccagat attggcgccg
      781 gcgttgacga tgaggaagag cctgaggctg aagctcaaga aatggaagag cctcaatacg
      841 aatcgaaagt ggacgaaaaa gacgacgacg acaatggctc ttcttcctcc aaaaagggtc
      901 atccttccga agacggagat agtacaagaa atggagagac gccgacagac cgacgaaata
      961 gtggggcgat tgaagagacg gcagacggtg agagctctgc gcagtcggct gctgagaaga
     1021 agaatagtgg agacgacggc aacggaggcg gcggtgaaat gtcgattttg ctcgtcaaaa
     1081 atgctcaaat tgtcaatgac gatgcgattt ttgtagctga tattctgatt gaagatggaa
     1141 ttattcaaaa cgtcgcccca aacctcgaag ctccagaagg cgccgaagtg ctcgacgccg
     1201 cgggaaagct cgcccttccc gccggaatcg atgtctacac tcaggtcacc gattcttccg
     1261 tcgatgatct ctcgactggc tgtaaatctg caatcgctgg cggtaccgga accatcgttg
     1321 aagtcgttcg gccacgtggc gccgaatcgg tggtctcggc tgttaagcgg gtgaaaaatc
     1381 aactggaaaa gtctggaatc tcgtgccacg tggcactttc tgtggcgatt acggatttct
     1441 gtgagcaaga aatgtcggag ctcgtgaaaa acgaaggaat caatagtttt gtgctcgacg
     1501 gtgtctccct gacagacgat aagctccttg agctctttga gcacgtaaaa cggctcggag
     1561 ccctaatccg tgtagtcccg gagaacaagt cgatcgtcgc gatgctggag aaaaagatgc
     1621 tgaagctcgg agtcactggg cccgaaggct tcccacagtc gcggcctgaa agcctggaag
     1681 ctgatagggt tagcggagtc tgtgtactcg ggaatctggc ttcctgtccg atttccattg
     1741 tgcaggtctc gtcagcagac tctctggcgg cgatagagaa ggcccgggct tcaggagctc
     1801 tggctcacgc tgagattgca tcggctgctg tgacggctga tggctcggca cttttcagtc
     1861 aggatttgag atttgcgtcg gctcatctga cggatgttcc actgagacgt ggggctccgg
     1921 atcggatgat cggagcactt tcgacgcagc ctctggtggt ttgtacgtcc ggacatcggc
     1981 cggtcaactc ggcgaccaga gtagcggcca aggattttgc catcgcgcag aagggatcta
     2041 cgggagccga agaacgcatg gcagtcgtat gggaacgggc ggtacgaagc ggtcgaatcg
     2101 atgcgatgcg tttcgtggcg gtcacctcaa cgaacgctgc gaaaatgttc aatatgtacc
     2161 cgaaaaaggg ccgaattgca gtcggcgccg acgctgatct cgtaatttgg gatgccagtg
     2221 gaaagcgagt cctcgagtcg agccgagcac agagctcaca ggagaattcg atgtacgacg
     2281 ggctcactgt gcattcggtg gtgacggcga cgattgttgg cggaaaaatt gcctatcaga
     2341 atggagaagt tcgggaggca ccggtagccg gaggttttct gcgtctctcg ccaaattctc
     2401 cctatctctt cagtatggtt gggcagaggg ataagttcgc caacgtggag cgtgtcgaac
     2461 gagaagcctc ctcacaacaa caaaaacccc aacaaaacgg tcaccacaag aattcgggag
     2521 attttgatcg aaatcgcacc aaagtaatgg aatcttccat cgattttggc ggctccgcgg
     2581 cgaatcggcc caggaatcca cccggcggac ggactacagg gttttggtag cccgaaagct
     2641 cctcgaaaaa tgccaaaaat tcataattta ctattactag tcataattgt attattctaa
     2701 tccatgtttc cccctccaca aaaaatgagc tttttctcca aatttccttt ttcccatcga
     2761 gaggaaaaag atattatcac cgcagaaaac aattttaaaa acattccact ttatttttag
     2821 tatttattta aatttttggc gttaaaatca tgtttttcga cttcaaaatg ctccaaaatc
     2881 actccaaaaa ttcgagaaac ggacaaaaaa ttcagttaaa atctgaattt ttttctgaaa
     2941 aataacgtgg tttcaggttg tcccatcacg gtgtgatcta caaaaatgcg ggaaatgaga
     3001 gaaaactctc gaattttttg tagatcaaac cgtgatggga caacctgaaa ccacgtgaaa
     3061 aattcgaatt tttttttgct aatttgcctg tttttcggat tttttttccc attttcacta
     3121 cgacaatctg tatttttgag ccattttttt gagtatttta acgttgaaaa atcgtatttt
     3181 gacgctaata tttattttac tttgtaataa tttataatta cacctgcata atttttttct
     3241 ttttttctat gagcttgtat tgatcttttt cgcatttttt ttgtacccct tcccctcgtt
     3301 tttaatgtcg atttttgcta ggtggccgcg aaattgtaaa aaatcggtca tgtgtcgatt
     3361 tacgcagctc gtgtagtctg aaaatctgaa aattctcata ttttcgttta aagttggttg
     3421 aaaaatagag aattttacat gaaaactgaa taatttgaga gcaaaaaaat ttaattttaa
     3481 ctgttttgca aaatttcgac tgaaattcat ctttattgca gcaaaaattg caaaaaatca
     3541 agcatttcag tcacaaaagc tccgcataaa tcgacctaaa acaccttttt cgaaccacat
     3601 ggcaaatgtc gatcccttcc cccaacttta cacataaata aaggaatttt atgggctaaa
//