From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: E. coli rhaT gene for L-rhamnose permease, rhaC (3' end) and sodA
Date: 28 Jul 92 10:02:45 GMT
Distribution: bionet
Checksum: 38811     5

LOCUS       ECORHAU      1661 bp ds-DNA             BCT       28-JUL-1992
DEFINITION  E. coli rhaT gene for L-rhamnose permease, rhaC (3' end) and sodA
            (5' end) genes
ACCESSION   X60699
KEYWORDS    L-rhamnose permease; rhaT gene.
SOURCE      Escherichia coli DNA.
  ORGANISM  Escherichia coli
            Prokaryotae; Gram-negative facultatively anaerobic rods;
            Enterobacteriaceae.
REFERENCE   1  (bases 1 to 1661)
  AUTHORS   Garcia,C., Baldoma,L., Badia,J. and Aguilar,J.
  TITLE     Nucleotide sequence of the rhaC-sodA interval specifying rhaT in
            Escherichia coli
  JOURNAL   J. Gen. Microbiol. 138, 1109-1116 (1992)
  STANDARD  full automatic
COMMENT     *source: strain=K12; *source: clone=pCG1;
            From EMBL    entry ECRHAT;  dated 01-JUL-1992.
FEATURES             Location/Qualifiers
     CDS             complement(<1..288)
                     /gene="sodA"
                     /codon_start=1
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQ"
     RBS             561..565
                     /gene="rhaT"
     CDS             573..1544
                     /product="L-rhamnose permease"
                     /gene="rhaT"
                     /evidence=EXPERIMENTAL
                     /codon_start=1
                     /translation="MSNAITMGIFWHLIGAASAACFYAPFKKVKKWSWETMWSVGGIV
                     SWIILPWAISALLLPNFWAYYSSFSLSTRLPVFLFGAMWGIGNINYGLTMRYLGMSMG
                     IGIAIGITLIVGTLMTPIINGNFDVLISTEGGRMTLLGVLVALIGVGIVTRAGQLKER
                     KMGIKAEEFNLKKGLVLAVMCGIFSAGMSFAMNAAKPMHEAAAALGVDPLYVALPSYV
                     VIMGGGAIINLGFCFIRLAKVKDLSLKADFSLAKSLIIHNVLLSTLGGLMWYLQFFFY
                     AWGHARIPAQYDYISWMLHMSFYVLCGGIVGLVLKEWNNAGRRPVTC"
     misc_feature    complement(1603..>1661)
                     /note="internal stop codon at1646-1644"
                     /gene="rhaC"
BASE COUNT      349 a    362 c    484 g    466 t
ORIGIN
        1 ctgcagggtg gtgccttttt tcagaccttt ccagaacagg ctgtggttag cgtgaccgcc
       61 agcgttgttg cgcagtacgg ttttcttgtc tgctggcagc tggtccagtt tggtgatcag
      121 ctcttcaacc ggcaggttgg caaattctgg caggctttcc agcgccgcgt tggcgttgtt
      181 tacgtaggtc tgatggtgtt tggtgtggtg gatttccatg gtctgcttat cgaagtgcgg
      241 ttccagggca tcgtaagcat acggcaggga tggcagggta tagctcatat tcatctccag
      301 tattgtcggg cggccgattg ttaatgccgc gtaagcagtt ggttcattat agttaattaa
      361 atgatattga aaatgattat caatgccgta cttttcgtaa gggtatggtt ttgcaggaaa
      421 atgcccgaga tgtgaagcaa atcacccact taatgccgtg attgccagta aatcgacaac
      481 ggcggcaaca ggcgaaaggt taatcgacag cacgattttt acactcatct cgtcggagat
      541 gtgacgcgac gaaaaatgat gaggataaga agatgagtaa cgcgattacg atggggatat
      601 tttggcattt gatcggcgcg gccagtgcag cctgttttta cgctccgttc aaaaaagtaa
      661 aaaaatggtc atgggaaacc atgtggtcag tcggtgggat tgtttcgtgg attattctgc
      721 cgtgggccat cagcgccctg ttactaccga atttctgggc gtattacagc tcgtttagtc
      781 tctctacgcg actgcctgtt tttctgttcg gcgctatgtg ggggatcggt aatatcaact
      841 acggcctgac catgcgttat ctcggcatgt cgatgggaat tggcatcgcc attggcatta
      901 cgttgattgt cggtacgctg atgacgccaa ttatcaacgg caatttcgat gtgttgatta
      961 gcaccgaagg cggacgcatg acgttgctcg gcgttctggt ggcgctgatt ggcgtaggga
     1021 ttgtaactcg cgccgggcag ttgaaagagc gcaagatggg cattaaagcc gaagagttca
     1081 atctgaaaaa agggctggtg ctggcggtga tgtgcggcat tttctctgcc gggatgtcct
     1141 ttgcgatgaa cgccgcaaaa ccgatgcatg aagccgctgc cgcacttggc gtcgatccac
     1201 tgtatgtcgc tctgccaagc tatgttgtca tcatgggcgg cggcgcgatc attaacctcg
     1261 gtttctgttt tattcgtctg gcaaaagtga aggatttgtc gctaaaagcc gacttctcgc
     1321 tggcaaaatc gctgatcatt cacaatgtgt tactctcgac actgggcggg ttgatgtggt
     1381 atctgcaatt ctttttctat gcctggggcc acgcccgcat tccggcgcag tatgactaca
     1441 tcagttggat gctgcatatg agtttctatg tattgtgcgg cggtatcgtc gggctggtgc
     1501 tgaaagagtg gaacaatgca ggacgccgtc cggtaacgtg ttgagcctcg gttgtgtggt
     1561 gattattgtc gccgctaaca tcgtcggcat cggcatggcg aattaatctt tctgcgaatt
     1621 gagatgacgc cactggctgg gcgtcatccc ggtttcccgg g
//