From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Bovine herpesvirus-4 (clone pH12PH4.2) IE2 protein (IE2) gene,
Date: 11 Sep 92 10:03:59 GMT
Distribution: bionet
Checksum: 64234     9

LOCUS       HSBIE2A      4039 bp ds-DNA             VRL       11-SEP-1992
DEFINITION  Bovine herpesvirus-4 (clone pH12PH4.2) IE2 protein (IE2) gene,
            complete cds; BRRF2 gene homologue, 5' end cds; BRRF1 gene
            homologue, partial cds.
ACCESSION   L01099
KEYWORDS    EBV BRRF1 product homologue; EBV BRRF2 product homologue;
            immediate-early IE2 protein.
SOURCE      Bovine herpesvirus type 4 (individual_isolate DN-599) kidney DNA.
  ORGANISM  Bovine herpesvirus type 4
            Viridae; ds-DNA enveloped viruses; Herpesviridae.
REFERENCE   1  (bases 1 to 4039)
  AUTHORS   van Santen,V.L.
  TITLE     Characterization of bovine herpesvirus-4 immediate-early RNA
            encoding homolog of Epstein-Barr virus R transactivator
  JOURNAL   Unpublished (1993)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     CDS             join(1200..1202,2137..3792)
                     /product="immediate-early IE2 protein"
                     /gene="IE2"
                     /note="putative"
                     /codon_start=1
                     /translation="MKGIIYPRKSSADRYVYLSPGVREHLFSLLVKYTSSQRDSPAGS
                     STKVAPDTPQTNFIVEIQWVCHKILLEMEEQFGSLGGLVADINICLIWTLFRNYKHKH
                     RMNNSDTGKSCAEYAQSVVKHLTERMVYCTDKFFINSACSGVTVPQNLALVIASISQV
                     CRNKCQGAWRRMGNGRRTLIDLGLQLVNTYNLLNACGAIDDKCKAFIKLTFPYLNLET
                     VYSPVHAASTGLHQKMAISLYKGQEKRKVPNATIYSNLVTQEKFALPEILLGEITDEG
                     LLANKGPDLEKLLSEPQTILKLVTKLSPVSQFQVFMARWGDKLPSHLKDVCVSDSSQL
                     PQQYENFKVVWPQHQTWPNDETSALPIASASLLSLQAENLPTSVQNSVAQETTGLPPQ
                     VPAGEMPPRGNCPVEDHGLLNSDAPDDLSGKGDYDLFTSDDLLPMSSGNVAIPVCEDP
                     LAPPCKKQRMEPPDPLSTPGDNQWGDGAASAWIQSYMENEDAYLELILQGLYHLDEPP
                     KLEDSIQVQDVSNSTNDPLEGPSRSQASCFEDTENIANPLDLFV"
     mRNA            join(1132..1202,2137..3956)
                     /note="'IE RNA 2'"
                     /gene="IE2"
     CDS             complement(<1217..>1990)
                     /function="R transactivator"
                     /note="homologue to Epstein-Barr virus BRRF1 gene"
                     /note="putative"
                     /codon_start=1
                     /translation="KPITALERALFLTDVAQILIQHKNQEKSIMVLIQDIIKYMITSL
                     NELNNADAQCSTPQLLHFLVSDDQMLGVHLIKLLPEILLCKYPNFSTSPINSGDMDYI
                     EWCLHHMLAISETDQNLLRNTLSTREWTLMPSIQVLCHIMQQLFLSQTHCPEYKDHEF
                     NLFFNQAIFWTTVLKMFQECIRQDMVGDHIQCVIALLKNEVRQFFLWYDINKIHFKPM
                     AMVKYLEHLVNSITYESSDILLFQARLLLSHCKLILQKAQ"
     CDS             complement(1..>996)
                     /function="R transactivator"
                     /note="homologue to Epstein-Barr virus BRRF2 gene"
                     /note="putative"
                     /codon_start=1
                     /translation="MEITVYIPGIEENQRPHFDEIFSTFAKRGNVREAVSSLHKLFKK
                     QKNTGLLASLVILKQFVDTSRKSQERFNLLEILQQTKFLAQSIYKHIKSQEHSCDMED
                     MFSDCKDRMSLILSESCGCLNCITTTKQLMNSLCDARPPKLSSHKKVCQAHNFLTTVH
                     NQVVVADRVSVSVLSLSDLVLDMGDFPELPKDIQLETRGVASCVYLCWFYYMLLKHVQ
                     NDFEILEASINNWLLTNGYSQGFTSYDNLLPIVTTKRGSGELTYNLDSFQVLQNTLQT
                     VRNFLTTQPPRREQKHLLTLLKEKGYYVEPKESMCKTPQCPEEEGGLPFLKKLGTG"
     exon            1132..1202
                     /number=X
                     /gene="IE2"
     intron          1203..2136
                     /note="'removed from IE RNA 2'"
     exon            2137..3956
                     /number=X+1
                     /gene="IE2"
     polyA_signal    3931..3936
                     /note="'for IE RNA 2'"
BASE COUNT     1193 a    790 c    809 g   1247 t
ORIGIN
        1 gccagtgcca agctttttaa gaaatggtag acccccttct tcctctggac actgtggagt
       61 tttacacatt gactcctttg gctcaacata ataccccttt tcctttaaca atgtcagcag
      121 atgcttttgc tcgcgacgtg ggggctgggt agtcaggaag tttctgactg tttggagtgt
      181 attttgcaga acttgaaatg aatccaggtt atatgttaat tccccactcc ctcgctttgt
      241 tgtgactata ggtaacaggt tatcatagct ggtaaatccc tgtgaatagc cattagtgag
      301 caaccagtta ttaatacttg cttccaatat ctcaaaatca ttttgaacat gttttaaaag
      361 catgtaataa aaccaacaca aataaacaca ggatgcgacc ccccttgtct ccagttggat
      421 atcttttggg agttctggaa aatcccccat gtccaagaca aggtcagata gtgagagcac
      481 tgaaactgag actctatctg ctaccaccac ctggttgtgg acagtagtta aaaagttatg
      541 tgcctgacaa acttttttgt gggatgagag cttagggggt ctggcatcac ataatgaatt
      601 cattagttgt tttgttgtgg ttatgcaatt taaacaccca catgattctg ataaaattaa
      661 agacattcta tctttacaat cagaaaacat atcctccata tcacaggaat gttcttgact
      721 tttgatgtgc ttgtatatgc tctgagctaa gaactttgtt tgttgtaaaa tctccaggag
      781 gttaaacctc tcttgagatt ttctggaggt atccacaaac tgttttaaaa taactaaaga
      841 agctaaaagc ccggtgttct tctgtttctt gaataattta tgaagtgaag aaacagcttc
      901 tctgacattt cccctttttg caaaagtact aaagatttca tcaaagtggg gtctttgatt
      961 ttcttcaata cccggaatat atacggtgat ttccatggtg agtctacgcc ctttaatcca
     1021 aaaggtatcc ctgaggttaa atatatatgt cagtcactgg tgagtaatgg tgatactcct
     1081 ccctggaata catcatgcca ataaccctgc ccacttttgt tcttttaaaa gggagcagaa
     1141 caacagatgg tatgagtgac tagagcttca gccacaagaa acaaacacac agaccagtca
     1201 tggtaagaat gctttattgt gctttttgta gtatcaattt gcaatgtgat aacagcagcc
     1261 tggcttgaaa taagagaata tctgagctct cgtatgttat actgttaact agatgttcta
     1321 agtattttac cattgccatg ggtttgaaat ggattttgtt gatatcatac catagaaaga
     1381 actgtctaac ttcatttttc aacagggcaa taacacattg tatgtgatct cctaccatat
     1441 cctgccttat gcactcttga aacattttta agacagttgt ccagaaaatt gcttgattga
     1501 aaaacagatt gaattcatga tctttatact ctgggcaatg tgtttgagat aaaaatagtt
     1561 gttgcatgat gtgacacaaa acttgtatgg aaggcatgag ggtccattct cttgttgata
     1621 gtgtatttct gagtaagttt tgatctgttt cagaaattgc caacatatgg tgcagacacc
     1681 attcaatata gtccatgtcc ccactgttta tgggacttgt ggaaaagttt ggatacttac
     1741 aaagaagtat ttctggcagt agttttataa ggtggacacc caacatttga tcatcagaaa
     1801 ctagaaaatg aagtaattgg ggggtactgc attgtgcatc agcattgttt aactcattta
     1861 aacttgtgat catatatttt ataatgtctt gaattagtac cataatagat ttttcttggt
     1921 ttttgtgctg aattaatatc tgggctacat ctgttaaaaa aagggccctt tccagagcag
     1981 tgattggttt ttataatttg tttccccaga gttaaaactc agcttgtcct tttctttaac
     2041 tatctgagat aggtcaggga aatgataatt tttgaactct gaaagaatct tattattgac
     2101 acctgtattc atattggtat cccttttaaa ttgcagaagg ggattattta tcccagaaaa
     2161 tcttcagcag atcgatatgt ctatttatca cccggagtca gagagcatct gttttcatta
     2221 ctggtgaagt acaccagctc ccaacgtgac tcaccagctg gctccagcac taaagtggca
     2281 ccagatacac ctcagacaaa ttttattgta gaaatacaat gggtttgtca taaaattttg
     2341 ttggagatgg aagaacaatt tggatctttg gggggtcttg tggcagacat caatatttgt
     2401 cttatatgga cactctttag aaactacaag cacaagcaca gaatgaataa ttcagacact
     2461 ggaaaatcat gtgcagaata tgctcaatct gttgtgaaac atctcacaga aagaatggtg
     2521 tattgcactg acaagttttt tataaactct gcgtgttctg gcgtcacagt tccacagaac
     2581 ctggccttag ttatagcctc tatttcacaa gtgtgccgga ataaatgtca gggggcgtgg
     2641 aggcggatgg ggaatggaag aaggactctg attgatttgg gtttacagct ggttaataca
     2701 tataaccttt tgaatgcatg tggggccatt gatgacaaat gtaaggcatt tataaagctc
     2761 acctttcctt accttaatct tgagactgta tactccccag tccatgctgc ctccacgggt
     2821 ctgcaccaaa aaatggctat ctctctttat aagggacagg aaaaaagaaa agtacccaat
     2881 gcaaccatct actcaaacct tgtaactcaa gaaaagtttg cccttccaga gattcttttg
     2941 ggggaaataa cagacgaggg tctcttagca aacaaaggac cagatctgga gaaactgtta
     3001 tctgaaccac agaccattct caaacttgta acaaaactga gcccggtgtc tcaatttcaa
     3061 gtgtttatgg caaggtgggg ggataaatta ccctcccatc ttaaagatgt atgtgtaagt
     3121 gactcctcac aactgcccca acaatatgaa aactttaagg ttgtgtggcc ccaacatcaa
     3181 acatggccta atgatgaaac ttcagctcta ccaattgctt cagcatcttt gttgtcactc
     3241 caggctgaga accttccaac aagtgtacaa aattcagtag cccaggaaac aacgggcctt
     3301 cccccacaag tacctgctgg ggaaatgccc ccccggggga attgtccagt agaggaccat
     3361 ggtttattga acagtgatgc cccggatgat ctctctggga agggggacta tgatttgttt
     3421 acaagtgatg atttgttgcc catgtcatct ggcaatgtag ctatacctgt ctgtgaggac
     3481 ccacttgccc ccccatgtaa gaagcaaagg atggaaccac cagatccctt atcgactccc
     3541 ggggacaacc agtggggtga tggtgctgct tctgcgtgga tacaatctta tatggaaaat
     3601 gaagatgcat acctagagct tattctacag ggactatatc acttggatga gccccccaaa
     3661 ttagaagaca gcattcaagt ccaagatgtc tccaattcta ccaatgaccc attggagggc
     3721 ccatccaggt cacaggcatc atgttttgag gacacagaaa atattgcgaa ccccctcgat
     3781 ttgtttgtgt aaatacttat aaaactgtaa ttgtgtatat atgatgaaag atgttgacaa
     3841 tagccttgat agattattgc tcaaacctat ctcattttaa gctattgata attattgtac
     3901 agttttcaaa tcaatgttat atgtttttca aataaagttt tacttggcag actacaagag
     3961 gctttggact ttgtatatat tatagtttca ttatgggcat tttatcatac ctctgcacct
     4021 ttcaactgac taactgcag
//