From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Tetrahymena pyriformis beta-tubulin gene 2 (beta-TT2).
Date: 24 Jul 92 10:10:20 GMT
Distribution: bionet
Checksum: 19980     5

LOCUS       TETTUBB2     2102 bp ds-DNA             INV       24-JUL-1992
DEFINITION  Tetrahymena pyriformis beta-tubulin gene 2 (beta-TT2).
ACCESSION   X12769
KEYWORDS    beta-tubulin; tubulin.
SOURCE      Tetrahymena pyriformis.
  ORGANISM  Tetrahymena pyriformis
            Eukaryota; Animalia; Protozoa; Ciliophora; Oligohymenophora;
            Hymenostomata; Hymenostomatida; Tetrahymenina; Tetrahymenidae.
REFERENCE   1  (bases 1 to 2102)
  AUTHORS   Barahona,I., Soares,H., Cyrne,L., Penque,D., Denoulet,P. and
            Rodrigues-Pousada,C.
  TITLE     Sequence of one alpha- and two beta-tubulin genes of Tetrahymena
            pyriformis
  JOURNAL   J. Mol. Biol. 202, 365-382 (1988)
  STANDARD  simple automatic
COMMENT     *source: strain=CGL; library=macronuclear DNA in pBR322; *source:
            clone=beta-TT2.  tag and taa encode Gln !
               EMBL features not translated to GenBank features:
               key        from     to       description
               PRM           5     11       TATA-box
               SITE         25     30       poly(AG) stretch
               SITE         36     36       pot. transcription start site
               SITE         40     40       pot. alt. transcription start site
               SITE         45     45       pot. alt. transcription start site
               SITE       1701   1701       transcript termination site
FEATURES             Location/Qualifiers
     CDS             129..1460
                     /note="beta-tubulin 2 (AA 1 - 443)"
                     /codon_start=1
                     /translation="MREIVHIQGGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQL
                     ERINVYYNEATGGRYVPRAILMDLEPGTMDSVRAGPFGQLFRPDNFVFGQTGAGNNWA
                     KGHYTEGAELIDSVLDVVRKEAEGCDCLQGFQITHSLGGGTGSGMGTLLISKVREEYP
                     DRIMETFSVVPSPKVSDTVVEPYNATLSVHQLVENADECMVIDNEALYDICFRTLKLT
                     TPTYGDLNHLVSAAMSGVTCCLRFPGQLNSDLRKLAVNLIPFPRLHFFMIGFAPLTSR
                     GSQQYRALTVPELTQQVFDAKNMMCAADPRHGRYLTASALFRGRMSTKEVDEQMLNVQ
                     NKNSSYFVEWIPNNIKSSICDIPPKGLKMAVTFVGNSTAIQEMFKRVAEQFTAMFRRK
                     AFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATAEEEGEFEEEEGEN"
BASE COUNT      646 a    475 c    363 g    618 t
ORIGIN
        1 aagcttaaat acaaaggaat ttggagagag gaggaagata atataataat ttagaagcgt
       61 gctttttaaa agagtagtta acattaataa aaaaaggaaa ataaccctaa aaaaatatca
      121 aaatcaagat gagagaaatc gttcacatcc aaggtggtca atgtggtaac caaatcggtg
      181 ccaagttctg ggaagtcatt tccgacgaac acggtatcga ccccactggt acctatcacg
      241 gtgactccga tttacaattg gaaagaatca acgtttacta caatgaagct actggtggta
      301 gatacgtccc cagagccatc ttgatggatt tggaacctgg taccatggac tccgtcagag
      361 ctggtccttt cggtcaactc ttcagacctg ataacttcgt tttcggtcaa actggtgctg
      421 gtaacaactg ggctaagggt cactacactg aaggtgctga attgatcgac tccgttttgg
      481 acgtcgtcag aaaggaagct gaaggttgcg attgcctcca aggtttctaa atcacccact
      541 ccctcggtgg tggtaccggt tccggtatgg gtaccctcct tatctccaag gtcagagaag
      601 aatatcccga tagaatcatg gaaaccttct ccgtcgtccc ctctcctaag gtctccgaca
      661 ccgtcgttga accctacaac gctactctct ctgtccatca attggtcgaa aacgccgacg
      721 aatgtatggt catcgataac gaagctctct acgatatctg cttcagaacc ctcaagctca
      781 ccacccccac ttatggtgat cttaaccact tggtctctgc tgccatgtcc ggtgttactt
      841 gttgcctcag attccccggt caattgaact ccgatctcag aaaactcgcc gtcaacttga
      901 ttcccttccc ccgtctccat ttcttcatga ttggtttcgc tccccttacc tccagaggtt
      961 ctcaacaata cagagccctc actgttcccg aattaaccca ataagtcttc gacgccaaga
     1021 atatgatgtg cgctgctgat cccagacacg gaagatattt gaccgcctcc gctctcttca
     1081 gaggtagaat gtccaccaag gaagtcgatg aacaaatgct caacgtccaa aacaagaact
     1141 cttcttactt cgttgaatgg attcccaaca acatcaagtc ctccatttgt gatattcctc
     1201 ctaagggtct taagatggct gttaccttcg ttggtaactc caccgccatc caagaaatgt
     1261 tcaagagagt cgccgaacaa ttcactgcta tgttcagaag aaaggctttc ttgcattggt
     1321 acactggtga aggtatggac gaaatggaat tcactgaagc tgaatccaac atgaacgatc
     1381 tcgtctccga atatcaataa tatcaagatg ccaccgctga agaagaaggt gaattcgaag
     1441 aagaagaagg tgaaaactga gaatctttaa attaacaatt caacaataca tatatttaca
     1501 caactttcat ataaaatata tataaaagta aattttttag tgttttatat taacataaaa
     1561 gcaccattaa acatgttaat atattactcc atattataat ttattcccaa ttgactaaaa
     1621 tctacgtctt aacatacaca cccctgttta catatgtttt atgtcgatta ctttttttaa
     1681 aacattccaa aaaaaaaaat tcctccttct tacatttatt tcttctttcc tctccttcct
     1741 taaatattta tatatggttg gataccagca gttgccttca ctgcattata agctccattg
     1801 ggtttgttta aaatactgtt acttaaaaaa aatatcattt ctcctgagtg tcttttctca
     1861 ttcgaatact tgttggattt tattgcagaa gctctccctc ttctaacgat cattaatcaa
     1921 ctgaattttg aaaactcatt ggattagttc cttgtaaatt atgtataaaa tgatgcagac
     1981 attctatcaa acagtgaata aatatgtata tctcctcttt catactctct aaactacaag
     2041 actgaatggc aatgtaaata aaatgaacaa tttcaagtgt ttcaagatga aaacaaagat
     2101 ct
//