From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Chlamydomonas reinhardtii plastocyanin (petE) gene, complete cds.
Date: 22 Dec 92 22:42:26 GMT
Distribution: bionet
Checksum: 13712 6

LOCUS       CREPETE      3380 bp ds-DNA             PLN       23-NOV-1992
DEFINITION  Chlamydomonas reinhardtii plastocyanin (petE) gene, complete cds.
ACCESSION   L07282
KEYWORDS    electron transfer catalyst; petE gene; plastocyanin.
SOURCE      Chlamydomonas reinhardtii (strain cw15) (library: lambda--EMBL3
            genomic) haploid DNA.
  ORGANISM  Chlamydomonas reinhardtii
            Eukaryota; Chlorophycota; Chlorophyceae; Volvocales;
            Chlamydomonadaceae.
REFERENCE   1  (bases 1 to 3380)
  AUTHORS   Quinn,J., Li,H.H., Singer,J., Morimoto,B., Mets,L., Kindle,K. and
            Merchant,S.
  TITLE     The plastocyanin-deficient phenotype of Chlamydomonas reinhardtii
            ac-208 results from a frame-shift mutation in the nuclear gene
            encoding
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     exon            1946..2264
                     /number=1
                     /gene="petE"
     intron          2265..2552
                     /gene="petE"
     polyA_signal    2805..2809
                     /gene="petE"
     CDS             join(1980..2264,2553..2705)
                     /gene="petE"
                     /product="plastocyanin"
                     /codon_start=1
                     /translation="MKATLRAPASRASAVRPVASLKAAAQRVASVAGVSVASLALTLA
                     AHADATVKLGADSGALEFVPKTLTIKSGETVNFVNNAGFPHNIVFDEDAIPSGVNADA
                     ISRDDYLNAPGETYSVKLTAAGEYGYYCEPHQGAGMVGKIIVQ"
BASE COUNT      672 a    964 c   1020 g    724 t
ORIGIN      
        1 ggtaccagct gtgcaacgtg tcgataagcg acgaccccaa gggcaaggtg agggagaagt
       61 cgggcacagg agcaaataaa cgcacgatca ggccccaaca ggttacagcc cttgttatgc
      121 cttgcgcctt gcccgcgact attgttctcg taaacccact gctctgctgt gaccaccggg
      181 tatattgtgc cgaccgtcgc atcgcgtgct gctactgctg ttgctactgt ctctgcagga
      241 cttgtttact ttcgcgcgct gcaaggcgcc gctgccgatc agcctactgc agaagacggg
      301 tgtgccccag ccggcggagc tggtggaggt cgtggagaag gacattgagt gggaggagtt
      361 caaggtgcgc aagcagggaa ggttgtcact gctttagggc gcagtcctta aagaggtcgc
      421 cgtgggtgct acatttgcat taggggaccc gggccgcgct ttttccttgg taactccccc
      481 gtttaagagc tgccgcctgc ttaacctgcg atgtgttatt catgctgtct gcagtggggc
      541 gtgtcctcgg tgaacctgcg cggcaaggag tacgtgctga agcggatgca cttcatgagc
      601 aacacacgcg ggggcgacct ggagctgtaa gcccgtgcag gcggggcggg gagacggcgc
      661 gtagttgggg cgtgaaagat tggcgatgtt gttgtgtgct atgttgctaa cagccagcac
      721 aacaggatga tgtggacatg ggcagtggga actgaggcat gagtctagag ctcgagactg
      781 gacagagggc agctagggag ctgaggctcg cgctggaggt ggaaatgttc gaagcaatgc
      841 gccatgcagt gcagggaagt accggatgtc gacggcgctc tatagatacg caggttgggc
      901 ctaggcccct cacgggccgt cgtatacacg acgagcccgc tatggttgcg gtgtagcctg
      961 gcgtggtggt cagtccgggt gaaccaggtc aaatgctact tgacgccagc tcatggcaac
     1021 ttaatgacga gtgttgcgga actaatgcgt acgcagacca gagcggaact tgtgctaatc
     1081 ggcattgtct cacacgtgtc agccctgggc tacggtgctt gccgccatgt tctaatccta
     1141 tgttgggcgc ctggggcgca agttgcagcg tggtctgtgg acggcgccat ttatccaagc
     1201 tgacacgaca aagtttctgg tttactatag gttacctgcc cctgaacatg tcatcctggg
     1261 tggtctcgcg aaggccgcgg gcatagtcct caggctcttg gcacgccctg aatacccatc
     1321 gcacaactgc tcctgcgtgt cataccccct gggccccttg cggtactgaa gtctctccct
     1381 ttcccaggtc taggctaccg tgcatcacca gatagcgtca cgcagtcaag cgcatgtaag
     1441 gaagggcagc acctggccag tgagtcaagg tttgcggaaa agcgcgctca tccacgtcac
     1501 gtcgcctcaa tccgtgaacc cagcaggcca ggatgattaa ttgcccgcat tctcatggag
     1561 ataaacgcga cctgagggca ccgagggacc tgggccaacc tccgggcgcg gatcgcatgc
     1621 gtgcagctac ctttcatcag gctgaggcgc atgccgcgcg tctaaattcg cgttgtaaag
     1681 gtgcatgcta attggaactg ggtcatgcac gtccgcggcc atttaaggac ctggcgcggc
     1741 tcacatgcag agtgctggac cgcgaacgtt cacaggaagc tccatagggt ccgtccaatc
     1801 gacacatgcc tttagattcc atgtcctagc accctcaaat tttgctgagc accaaaacct
     1861 gcggaaatcg cctcccaatc ccaaaagtac gacccatgca catctggttc gcccgggctc
     1921 aaattctccg cgcgctccca aacaaaccat acctggaaac acctcgtatc actttaaaaa
     1981 tgaaggctac tctgcgtgcc cccgcttccc gcgccagcgc tgtgcgcccc gtcgccagcc
     2041 tgaaggccgc tgctcagcgc gtggcctcgg tcgccggtgt gtcggttgcc tctctggccc
     2101 tgaccctggc tgcccacgcc gacgccaccg tcaagctggg cgctgactct ggtgctctgg
     2161 agttcgtccc caagaccctg accatcaagt ccggcgagac cgtgaacttc gtgaacaacg
     2221 ctggcttccc ccacaacatc gtcttcgacg aggatgccat ccccgtgagt ctatcatggg
     2281 ttcatggttt caattggccg ggcagatcac gcagtttggt tggctccgga tgtgcgaatc
     2341 gcatttgctt gcacagcgac ataacgccga cagctcgcgc gaattcgtgt ttgctgtgca
     2401 cattgcgcta gatttgcttg atcagtcgta ggggactccc aatccaccgt ctatcgttag
     2461 cggttcagcg gggtgccacg tcctcagcga catgcgacac gtccactgcc gcgactgact
     2521 ttcctctccc tcccctctct atctatgtgc agtccggcgt gaacgctgat gccatctccc
     2581 gcgatgacta cctgaacgcc cccggcgaga cctactcggt gaagctgacc gctgccggcg
     2641 agtacggcta ctactgcgag ccccaccagg gcgctggcat ggtcggcaag atcattgtcc
     2701 agtaaattgc tggcggctgc cttcattttg tgaccgtgtg tgtttcgggg tgtggggtcg
     2761 ggggtttttg cggcgtccgg atggacgcag agagcgtgta gctctgtaac tttttcctcg
     2821 gacaatttct cttgagcctc gtgcccctcg ctctgggttc ggatgggtga cagctgtctc
     2881 agtggggggt aggtgcagct gaggggcaag aatacacggg aagggtaggg gtgacagcgc
     2941 cagaacaagg acatcggaag cataggggac cacgggaggg ataggttggc ttgctcagac
     3001 agggttaagc gctgggtcac acagtaggac agcctgaaac gaaggtcact tcagcgcata
     3061 cgcggcgtga cagatgacaa gtttcagaca gatgcgggat ggtccccttc acagcgggtg
     3121 tgcgtgtgac agcatggtac ggtcgtgttc ccatgggctg ttgtccgcat gggtggtgtg
     3181 attgcgattg cccactgacc aagaccagtc acgcagcaaa gcgcggaata caaccttgct
     3241 tgttctcgaa gaaccatggc tgcttgaacg gtgcatagcg tcatagcgtg cgtcaaaccg
     3301 caggcttgcg cccgtcacgc caagccacac gcctgcctgt gcctcagtca aacacgtgct
     3361 ccagctcgaa gcccggatcc
//