From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Rhodococcus sp. beta-ketoacyl synthase 1 gene, 3' end;
Date: 26 Aug 92 10:00:51 GMT
Distribution: bionet
Checksum: 15287     6

LOCUS       RERCOABETA   1976 bp ds-DNA             BCT       26-AUG-1992
DEFINITION  Rhodococcus sp. beta-ketoacyl synthase 1 gene, 3' end;
            propionyl-CoA carboxylase beta chain gene, complete cds.
ACCESSION   M95713
KEYWORDS    3-oxoacyl synthase I; acyl carrier protein; beta-ketoacyl synthase;
            propanoyl CoA:carbon dioxide ligase;
            propionyl-CoA carboxylase beta chain.
SOURCE      Rhodococcus sp. (strain NI86/21) DNA.
  ORGANISM  Rhodococcus sp.
            Prokaryotae; Firmicutes; Nocardioform actinomycetes.
REFERENCE   1  (bases 1 to 1976)
  AUTHORS   Nagy,I., Schoofs,G., Vanderleyden,J. and De Mot,R.
  TITLE     Nucleotide sequence of a Rhodococcus gene encoding a protein with
            extensive homology to the mammalian propionyl-CoA carboxylase beta
            chain
  JOURNAL   Unpublished (1992)
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     CDS             2..124
                     /EC_number="2.3.1.41"
                     /standard_name="3-oxoacyl-[acyl-carrier-protein] synthase
                     I"
                     /note="similar to C-terminal regions of other eukaryotic
                     and prokaryotic beta-ketoacyl synthases; includining
                     nodulation protein E (NodE) from Rhizobium species"
                     /product="beta-ketoacyl synthase 1"
                     /note="putative"
                     /codon_start=1
                     /translation="DPEIDLDVVKGEPRTGSFDFALNNSFGFGGHNVALAFGRA"
                     /citation=[1]
                     /partial
     stem_loop       145..185
     RBS             221..227
                     /note="based on E. coli and B. subtilis Shine-Dalgarno
                     consensus sequences"
                     /note="putative"
                     /citation=[1]
     CDS             233..1663
                     /EC_number="6.4.1.3"
                     /standard_name="propanoyl-CoA:carbon dioxide ligase"
                     /note="similar to rat and human propionyl-CoA carboxylase
                     beta subunits"
                     /product="propionyl-CoA carboxylase beta chain"
                     /note="putative"
                     /codon_start=1
                     /translation="MTILAPVTKSESSTDPRDPLARLENLFDPGTTVPLHARDKSGVL
                     AASGNIDGVRTIAYCSDATVMGGAMGVDGCKHLVKAIDTAIEEESPIVGLWHSGGARL
                     AEGVEALHAVGLVFEAMVRASGLIPQISVVLGFAAGGAAYGPALTDVVIMAPEGRVFV
                     TGPDVVRSVTGEQVDMVSLGGPDTHTKKSGVAHIAAHDEADALHRARRLVSMMCEQGE
                     FDQRAAELGDSDLRAMMPASAKRAYDVRPIVHEMLDNVEGESSFEELQGNYARSIVTG
                     FGRMAGRTVGVIANNPLRLGCLNSESAEKAARFVRLCNAFGVPLVVVVDVPGYLPGVS
                     MEWEGVVRRGAKLLHAFAEATVPRVTVVTRKIYGGAYIAMNSRALGATAVFAWPNSEV
                     AVMGAKAAVGILHKRALAAAPDDEREALHDRLAAEHEAIAGGVDRRCRNRRRRRGNRP
                     GEDSQHRHAALASAPSVRARHKNIPL"
                     /citation=[1]
     stem_loop       1678..1712
BASE COUNT      369 a    650 c    585 g    372 t
ORIGIN
        1 ggatccagaa atcgatctcg atgtcgtcaa gggtgaaccc cgaaccggct ctttcgattt
       61 cgcactgaac aactcgttcg gtttcggtgg gcacaatgtc gcgctcgcct tcggtcgggc
      121 ctagcccgaa ccgcggtttg cacctgtccg cgggtgtcgt tgcaacacaa cgtcacccgc
      181 ggacaacacc gacctttcgt tccacccaag gctcgtactg aaggaggacg cgatgaccat
      241 cctggccccc gtgacaaagt ccgaatcatc gacagacccg cgcgatcctc ttgcgcgcct
      301 ggaaaatctg ttcgaccccg gcacaacagt gccgctccac gcccgtgaca agtccggcgt
      361 gctcgctgca tccggaaaca tcgacggcgt ccgcaccatc gcatactgct ccgatgccac
      421 cgtcatgggc ggcgcaatgg gcgtcgacgg ttgcaaacac ctcgtcaaag ccatcgatac
      481 cgccatcgag gaagagtccc ccatcgtggg tctctggcac tccggtggcg cgcgtctggc
      541 tgaaggcgtc gaagcactcc acgcagtcgg actggtcttc gaggccatgg tccgtgcctc
      601 cggtctcata cctcaaattt ccgtcgtgct cggctttgcc gctggtggag ccgcgtacgg
      661 tccagccctc accgatgtcg tgatcatggc gccggaaggc cgagtgttcg tcaccggccc
      721 agacgtagtt cgcagtgtca ccggtgaaca ggtcgacatg gtgtcactcg gcggtccgga
      781 cacacacacc aagaagtcgg gtgtcgcgca catcgccgca cacgacgagg ccgacgcatt
      841 gcaccgcgca cgccgcctgg tctcgatgat gtgcgagcag ggcgaattcg atcagcgggc
      901 agcggaactc ggcgacagcg atctgcgcgc catgatgcct gcgtccgcaa aacgtgcata
      961 cgacgttcgt cccatcgttc acgaaatgct cgacaacgtc gaaggcgagt cgagcttcga
     1021 agaactgcaa ggcaattacg cgcggagcat cgtcaccggt ttcggccgga tggccggacg
     1081 cacggttggc gtcatcgcga acaacccact gcgactcggg tgcctgaact ccgaaagtgc
     1141 agagaaggcc gctcgattcg tgcgactgtg caacgcattc ggcgtgccgc tcgtagtcgt
     1201 cgtcgatgtt cccgggtatc tgcctggcgt gtcgatggag tgggaaggcg tggttcggcg
     1261 cggcgcgaag ttacttcacg ccttcgccga ggcgactgtc ccccgcgtca ccgtcgtcac
     1321 tcgaaagatc tacggcggcg catacatcgc aatgaactcg cgagcactgg gagctacggc
     1381 cgttttcgct tggcccaact ccgaagtggc cgtgatgggc gcgaaggctg ctgtcggcat
     1441 actgcacaag cgggctctcg ctgcggcacc ggacgacgag cgtgaagcat tgcacgaccg
     1501 tctcgctgcc gaacacgagg cgatcgcagg cggcgtcgac cgtcgctgtc gaaatcggcg
     1561 tcgtcgacga ggaaatcgac ccggcgaaga ctcgcagcat cgtcacgctg cgctcgcgtc
     1621 tgcaccatct gtccgtgcgc gccacaagaa cattccgctc tgacaaccct ggacagcaag
     1681 gtgggcctcg ccgatacggc ggggcccacc tttgtcattc aagtctttgt ctgcacgtct
     1741 gtgtcattcc agctaaaggg tgccccgagt ttcaggatgg acctcggtca gctgacacgc
     1801 cttcgcagcc atgtcacttc ggcgccttcg cccgaactcc ggaagggttc gagttcctcg
     1861 tcccacggtg cgcctatcgc ccgatcgatc tccgcggcga tgtctccacc ggaattcatg
     1921 atggaccgca gtttcatctc cccaacgacg acatcaccgt tcgcactggt ggatcc
//