From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: H.vulgare gene for 1,3-glucan endohydrolase isoenzyme III
Date: 28 Dec 92 21:26:03 GMT
Distribution: bionet
Checksum: 28469 5

LOCUS       BLY13GEIII   2349 bp ds-DNA             PLN       01-DEC-1992
DEFINITION  H.vulgare gene for 1,3-glucan endohydrolase isoenzyme III
ACCESSION   X67099
KEYWORDS    glucan endo-1,3-beta-glucosidase.
SOURCE      Hordeum vulgare DNA.
  ORGANISM  Hordeum vulgare
            Eukaryota; Plantae; Embryobionta; Magnoliophyta; Liliopsida;
            Commelinidae; Cyperales; Poaceae.
REFERENCE   1  (bases 1 to 2349)
  AUTHORS   Wang,J., Xu,P. and Fincher,G.B.
  TITLE     Purification characterization and gene structure of
            (1-3)-beta-glucanase isoenzyme GIII from barley (Hordeum vulgare)
  JOURNAL   Eur. J. Biochem. 209, 103-109 (1992)
  STANDARD  full automatic
COMMENT     *source: strain=cv. NK 1558;
               From EMBL    entry HV13GEIII;  dated 15-OCT-1992.
FEATURES             Location/Qualifiers
     mRNA            join(691..797,978..>1903)
                     /partial
     prim_transcript 691..>1900
     exon            691..797
                     /number=1
     intron          798..977
                     /number=1
     exon            978..>1903
                     /number=2
     CDS             join(731..797,978..1903)
                     /EC_number="3.2.1.39"
                     /product="glucanendo-1,3-beta-glucosidase"
                     /codon_start=1
                     /translation="MARKGVDVAVALVLVALAAFPAVHSIGVCNGVLGNNLPAPSDVV
                     TLYRSKRIDAMRIYEPESKVLTALSGTGIAVLMDVGPALPSLASSPSAAAAWVKANVS
                     SFPGVSFRYIAVRNEVMDSAGQSTILPAMRNVQRALAAAGSPIKVSTSVRFDVFNNTS
                     PPSNGVLADKSGFLRPILNFLARPARPLLANVYPYFAYKGNPRDIQLTFATFVPGSTT
                     VNDNGLTYTNLFDAMVDSIYAALEKAGTPGVKVVISESGWPSDQGFGATAQNARAYNQ
                     GLINHVGNGSPKKAGALESYIFAMFNENLKDGDELEKNFGLFKPNMSPAYAITF"
BASE COUNT      590 a    636 c    598 g    525 t
ORIGIN      
        1 tctagaagcg aagggagtat taagtatgta tgaggaaccc tacgccttac gagaaattta
       61 gggatcatat atatatttgg ccagcccaat tttaaatact ctataacgtg ctctggctgc
      121 cactatgatg cttgcaccgt ggtgggtcga gggagggcta tgatagacga ttcgatcggg
      181 tgtgaaccac caccactctc ggtcctggtt gataacaccc gtgccacaag gtacacacag
      241 acaattagca tagcttgggg ggttagtggc aaggaagcaa caaagcacgc actgtggagg
      301 ccgccttcga cggccggtca tggtcagcga ggcgaggaaa ttccacgagc cagacaagct
      361 ctgcatatgt gcccaagcga tggaatcgaa cctgtaccgg cacgtcgaag acctcatcgt
      421 tcacggacgg atcgagtcaa tcaccgaacg cgcctacgat aaattatggc cgaactggat
      481 ggtgacaacc gctcaacccg attccacaga ccaacgaaca agttgcgccg gcggccggtc
      541 tcatctcatg ccagcactac acgttctgtg gatgctatgg aaaatcgaaa cagataagga
      601 actaataatg gtgtattatt gcgccaaact gccgggtagt tccgagaatt ttttgtgtat
      661 ataagagagc gaatgtgctt ggtacattgc atccaaacaa gggggaagac acctcaaaga
      721 tacacagagg atggcgagga aaggtgtaga cgtcgcagtg gcgctggtcc ttgttgccct
      781 cgcagcattt ccagcgggta atatatatgc ttactacgat gtgtatattt tcttggaatc
      841 cttcatacat aattagcaca caatagtatg cttcatctgt acatagtatt tgagctcgac
      901 tacatgttgt acctgtacct aacgtcgcaa caaataaaac tcatttggct cttgcatgcc
      961 gcatttttgt tcttcagtgc actccatcgg cgtctgcaac ggcgtgctcg gcaacaacct
     1021 gccggcgccg agcgacgtcg tgacgctcta ccgatccaag cgcatcgatg ccatgcggat
     1081 ctacgagccg gagagcaaag ttctcacggc gctcagcggc acgggcatcg ccgtcctcat
     1141 ggacgtcggc cccgcgctac ccagccttgc ctccagcccc tccgctgcgg ccgcctgggt
     1201 aaaggccaac gtctcctcct tcccgggcgt gtcgttccgc tacatcgccg tacggaacga
     1261 ggtcatggac agcgccggcc agagtaccat cctcccggcc atgaggaacg tgcaacgggc
     1321 gctcgcggcc gccggctcgc cgatcaaggt gtccacgtcg gtgcggttcg acgtcttcaa
     1381 caacacttcc ccaccctcca acggtgtttt ggcggacaag tcagggttcc tgaggcccat
     1441 ccttaacttc ctggcgagac cggcgcgacc gctgctggcc aacgtgtacc cctacttcgc
     1501 ctacaagggc aacccgcggg acatccagct tacgttcgcc accttcgtgc caggcagcac
     1561 caccgtgaat gacaacgggt tgacatacac caacctcttc gacgccatgg tcgactccat
     1621 ctacgccgcg ctggagaaag ccggcacacc cggggttaag gtggtcatat cggagagcgg
     1681 gtggccgtcg gaccaagggt tcggggcgac ggcgcagaac gcgcgggcgt ataaccaggg
     1741 gttgatcaac cacgtcggca atggctcgcc caagaaggcc ggggcgttgg agagctatat
     1801 tttcgccatg ttcaacgaga acctcaagga cggggacgag ctcgagaaga actttgggct
     1861 gttcaagccg aatatgtcac cggcctacgc cattactttc tagtgcaacg aaactcgata
     1921 tttcgcgttc gaataagctg cctggtaatg caattatgca aactgcatgc gtatcagtca
     1981 accactagtc tgtgttcacc agcaaaagaa aataaccact cgtgtgtgtc atgttttgtt
     2041 tctttcttaa atatccaata aaacaggtcg aaaaaaaagg attattccgc ggcgcgcctc
     2101 ctcccacctg ctcccaattc cgaggaatgc ttgaccgctt ttgataaata atgtgtgtat
     2161 gcaaacaaag ttttcttgaa tccctcagaa gttgagaaat tgaagcgtca agttcgtgat
     2221 cgaaagccaa ccatacccta ttatgtatgc accattcaag aagctcacgt gatacctaag
     2281 aaggcaaaaa tggtaaaaag catcaagaag catctttttc gataaattgt ctttgaaagt
     2341 ttccttttt
//