From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: B.subtilis sub gene for subtilisin
Date: 21 Dec 92 20:17:22 GMT
Distribution: bionet
Checksum: 19576 5

LOCUS       BACSUC       2021 bp ds-DNA             BCT       18-NOV-1992
DEFINITION  B.subtilis sub gene for subtilisin
ACCESSION   X63533
KEYWORDS    sub gene; subtilisin.
SOURCE      Bacillus subtilis DNA.
  ORGANISM  Bacillus subtilis
            Prokaryotae; Firmicutes; Endospore-forming rods and cocci;
            Bacillaceae.
REFERENCE   1  (bases 1 to 2021)
  AUTHORS   Davail,S., Feller,G., Narinx,E. and Gerday,C.
  TITLE     Sequence of the subtilisin-encoding gene from an antarctic
            psychrotroph Bacillus TA41
  JOURNAL   Gene 119, 143-144 (1992)
  STANDARD  full automatic
COMMENT     *source: strain=TA 41; *source: cell_line=E.coli RR1; *source:
            clone_library=expression library; *source: clone=pSUB41;
               From EMBL    entry BSSUB;  dated 13-OCT-1992.
FEATURES             Location/Qualifiers
     -35_signal      504..508
     -10_signal      523..528
     RBS             531..537
     stem_loop       1816..1846
     CDS             544..1803
                     /EC_number="3.4.21.14"
                     /gene="sub"
                     /product="subtilisin"
                     /codon_start=1
                     /translation="MKRSGKIFTTAMLAVTLMMPAIGVSANRGNAADGNEKFRVLVDS
                     ANQNNLKNVKEQYGVHWDFAGEGFTTNMNEKQFNALQNNKNLTVEKVPELEIATATNK
                     PEALYNAMAASQSTPWGIKAIYNNSNLTSTSGGAGINIAVLDTGVNTNHPDLSNNVEQ
                     CKDFTVGTNFTDNSCTDRQGHGTHVAGSALANGGTGSGVYGVAPEADLWAYKVLGDDG
                     SGYADDIAEAIRHAGDQATALNTKVVINMSLGSSGESSLITNAVDYAYDKGVLIIAAA
                     GNSGPKPGSIGYPGALVNAVAVAALENTIQNGTYRVADFSSRGHKRTAGDYVIQKGDV
                     EISAPGAAVYSTWFDGGYATISGTSMASPHAAGLAAKIWAQSPAASNVDVRGELQTRA
                     SVNDILSGNSAGSGDDIASGFGFAKVQ"
BASE COUNT      585 a    421 c    507 g    508 t
ORIGIN      
        1 gcatgctgcc ggtttagcag cgaaaatctg ggcgcaatat ccgtccgctt caaatgttga
       61 tgtgagaggc gaattgcaat accgcgctta cgaaaacgac atcctatccg gttactatgc
      121 tggatacgga gatgattttg cttcagggtt cggatttgca acagttcaat aagatcatca
      181 gtttgggcag ttccggtgtt tcgccggagc tgtttttata tggggttatt taaacgatcg
      241 gacataaaag gggaaagcgt taatacagtt tccgtattca aggtatatct tgaatatttt
      301 gcacctgtta aattttagtg gcagaaaact gaaatgggct tctacacagt cgtcattatg
      361 atcgggacct tttacccgtt aattagtccc gaatttagaa atgggacaaa tttcgacaat
      421 atttttccca attacgctat atctattctt ctgctaacgt tagatgtgtg gcaggaggtt
      481 gcgggacgcc gcggcaccag gaattaatgc cataaaaaac catataggat aggagattga
      541 tgtatgaaga gatcaggaaa gattttcaca acggcaatgt tggctgtcac tttaatgatg
      601 cctgcaatag gggtaagtgc aaacagagga aacgcagcag atggcaatga aaagtttcgg
      661 gtactggttg actcagccaa tcagaacaac ctgaagaacg tgaaagagca gtacggggta
      721 cactgggact tcgcaggtga aggttttact accaatatga atgaaaagca attcaatgct
      781 ctgcaaaaca ataaaaacct gacagtggaa aaagttccgg aattggagat tgccacggca
      841 acaaacaaac cggaagcttt atataacgca atggcggctt cccagtctac accttggggc
      901 attaaagcaa tttataataa tagcaatctt accagcacga gcggtggagc cggcatcaat
      961 attgcggtcc tggatactgg agtcaacacg aatcaccccg accttagcaa taatgtagaa
     1021 cagtgcaaag attttaccgt tggaacaaac tttacagata acagctgtac tgaccgccag
     1081 gggcatggga cacatgttgc aggatcggcg ttagctaatg gcggcactgg aagcggcgtt
     1141 tacggcgttg caccggaagc ggatctctgg gcttacaaag tacttggcga tgatggctct
     1201 ggttatgccg acgatatcgc ggaggcaatt cgccacgccg gtgatcaggc aactgccctt
     1261 aatacaaaag ttgtcatcaa tatgtccctt ggttcttcag gtgaaagcag cctgatcacg
     1321 aatgcagttg actatgccta cgacaaaggt gttctgatca ttgcggcagc aggaaactca
     1381 ggtccaaaac caggttcaat tggttatccg ggtgcgctgg ttaacgcagt ggcagttgca
     1441 gctcttgaaa atacaattca gaatggcact tatcgtgtag cagatttctc atcccgcgga
     1501 cataaacgga ctgcaggaga ttatgtaatc caaaaaggcg acgtggaaat ttcagcgcct
     1561 ggagcggcag tctattccac atggtttgat gggggctacg cgacaatcag cggaacttca
     1621 atggcttcac cgcacgcagc cggtttggca gcaaaaatct gggcgcaatc tccggcagct
     1681 tcgaatgtgg atgttagagg cgaattgcaa acccgcgctt ctgtaaacga tatcctatcc
     1741 ggcaatagcg caggatccgg cgatgatatt gcttcaggat tcggatttgc aaaagttcaa
     1801 taagatcaac agtttgggca gttccggtgt ttcgccggag ctgtttttat gtggtactat
     1861 ttatttaatc tgaaaaaaag ggtgaggcta tgaatatcgg atgcgtgatt aaatattacc
     1921 ggtcaaaaaa caacatgacc aatcacaatt ggccgacggc atttgctcta tttcacatct
     1981 cagaagatcg agtccaatat agatacgccg catgagtcga c
//