From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human myeloperoxidase mRNA, complete cds.
Date: 5 Nov 92 23:11:21 GMT
Distribution: bionet
Checksum: 07721 6

LOCUS       HUMMYP       2568 bp ss-mRNA            PRI       30-JUN-1987
DEFINITION  Human myeloperoxidase mRNA, complete cds.
ACCESSION   J02694
KEYWORDS    glycoprotein; myeloperoxidase; peroxidase.
SOURCE      Human (HL-60 cells) cDNA to mRNA, clone lambda-GM706.
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 2568)
  AUTHORS   Morishita,K., Kubota,N., Asano,S., Kaziro,Y. and Nagata,S.
  TITLE     Molecular cloning and characterization of cDNA for human
            myeloperoxidase
  JOURNAL   J. Biol. Chem. 262, 3844-3851 (1987)
  STANDARD  full automatic
COMMENT     Draft entry and computer-readable sequence of [1] kindly provided
            by S.Nagata, 18-FEB-1987.
               Although two other ATG codons were found at positions 182-184
            and 227-229, the codon at 149-151 was confirmed as the initiation
            codon since 'the first ATG codon is usually the initiation codon of
            eukaryotic mRNAs' [1].
FEATURES             Location/Qualifiers
     mat_peptide     149..982
                     /product="myeloperoxidase light chain"
                     /gene="MPO"
                     /codon_start=1
     mat_peptide     983..2383
                     /product="myeloperoxidase heavy chain"
                     /gene="MPO"
                     /codon_start=1
     CDS             149..2386
                     /EC_number="1.11.1.7"
                     /gene="MPO"
                     /product="myeloperoxidase"
                     /codon_start=1
                     /translation="MGVPFFSSLRCMVDLGPCWAGGLTAEMKLLLALAGLLAILATPQ
                     PSEGAAPAVLGEVDTSLVLSSMEEAKQLVDKAYKERRESIKQRLRSGSASPMELLSYF
                     KQPVAATRTAVRAADYLHVALDLLERKLRSLWRRPFNVTDVLTPAQLNVLSKSSGCAY
                     QDVGVTCPEQDKYRTITGMCNNRRSPTLGASNRAFVRWLPAEYEDGFSLPYGWTPGVK
                     RNGFPVALARAVSNEIVRFPTDQLTPDQERSLMFMQWGQLLDHDLDFTPEPAARASFV
                     TGVNCETSCVQQPPCFPLKIPPNDPRIKNQADCIPFFRSCPACPGSNITIRNQINALT
                     SFVDASMVYGSEEPLARNLRNMSNQLGLLAVNQRFQDNGRALLPFDNLHDDPCLLTNR
                     SARIPCFLAGDTRSSEMPELTSMHTLLLREHNRLATELKSLNPRWDGERLYQEARKIV
                     GAMVQIITYRDYLPLVLGPTAMRKYLPTYRSYNDSVDPRIANVFTNAFRYGHTLIQPF
                     MFRLDNRYQPMEPNPRVPLSRVFFASWRVVLEGGIDPILRGLMATPAKLNRQNQIAVD
                     EIRERLFEQVMRIGLDLPALNMQRSRDHGLPGYNAWRRFCGLPQPETVGQLGTVLRNL
                     KLARKLMEQYGTPNNIDIWMGGVSEPLKRKGRVGPLLACIIGTQFRKLRDGDRFWWEN
                     EGVFSMQQRQALAQISLPRIICDNTGITTVSKNNIFMSNSYPRDFVNCSTLPALNLAS
                     WREAS"
BASE COUNT      513 a    810 c    755 g    490 t
ORIGIN      223 bp upstream of PstI site.
        1 gtccttggaa gctggatgac agcagctggc aaggggataa gagagcagtg agcccctccc
       61 tcaaggaggt ctggctttat ccatagacag ggccctctga ggtggggctg aggtacaaag
      121 ggggattgag cagcccagga gaagagagat gggggttccc ttcttctctt ctctcagatg
      181 catggtggac ttaggacctt gctgggctgg gggtctcact gcagagatga agctgcttct
      241 ggccctagca gggctcctgg ccattctggc cacgccccag ccctctgaag gtgctgctcc
      301 agctgtcctg ggggaggtgg acacctcgtt ggtgctgagc tccatggagg aggccaagca
      361 gctggtggac aaggcctaca aggagcggcg ggaaagcatc aagcagcggc ttcgcagcgg
      421 ctcagccagc cccatggaac tcctatccta cttcaagcag ccggtggcag ccaccaggac
      481 ggcggtgagg gccgctgact acctgcacgt ggctctagac ctgctggaga ggaagctgcg
      541 gtccctgtgg cgaaggccat tcaatgtcac tgatgtgctg acgcccgccc agctgaatgt
      601 gttgtccaag tcaagcggct gcgcctacca ggacgtgggg gtgacttgcc cggagcagga
      661 caaataccgc accatcaccg ggatgtgcaa caacagacgc agccccacgc tgggggcctc
      721 caaccgtgcc tttgtgcgct ggctgccggc ggagtatgag gacggcttct ctcttcccta
      781 cggctggacg cccggggtca agcgcaacgg cttcccggtg gctctggctc gcgcggtctc
      841 caacgagatc gtgcgcttcc ccactgatca gctgactccg gaccaggagc gctcactcat
      901 gttcatgcaa tggggccagc tgttggacca cgacctcgac ttcacccctg agccggccgc
      961 ccgggcctcc ttcgtcactg gcgtcaactg cgagaccagc tgcgttcagc agccgccctg
     1021 cttcccgctc aagatcccgc ccaatgaccc ccgcatcaag aaccaagccg actgcatccc
     1081 gttcttccgc tcctgcccgg cttgccccgg gagcaacatc accatccgca accagatcaa
     1141 cgcgctcact tccttcgtgg acgccagcat ggtgtacggc agcgaggagc ccctggccag
     1201 gaacctgcgc aacatgtcca accagctggg gctgctggcc gtcaaccagc gcttccaaga
     1261 caacggccgg gccctgctgc cctttgacaa cctgcacgat gacccctgtc tcctcaccaa
     1321 ccgctcagcg cgcatcccct gcttcctggc aggggacacc cgttccagtg agatgcccga
     1381 gctcacctcc atgcacaccc tcttacttcg ggagcacaac cggctggcca cagagctcaa
     1441 gagcctgaac cctaggtggg atggggagag gctctaccag gaagcccgga agatcgtggg
     1501 ggccatggtc cagatcatca cttaccggga ctacctgccc ctggtgctgg ggccaacggc
     1561 catgaggaag tacctgccca cgtaccgttc ctacaatgac tcagtggacc cacgcatcgc
     1621 caacgtcttc accaatgcct tccgctacgg ccacaccctc atccaaccct tcatgttccg
     1681 cctggacaat cggtaccagc ccatggaacc caacccccgt gtccccctca gcagggtctt
     1741 ttttgcctcc tggagggtcg tgctggaagg tggcattgac cccatcctcc ggggcctcat
     1801 ggccacccct gccaagctga atcgtcagaa ccaaattgca gtggatgaga tccgggagcg
     1861 attgtttgag caggtcatga ggattgggct ggacctgcct gctctgaaca tgcagcgcag
     1921 cagggaccac ggcctcccag gatacaatgc ctggaggcgc ttctgtgggc tcccgcagcc
     1981 tgaaactgtg ggccagctgg gcacggtgct gaggaacctg aaattggcga ggaaactgat
     2041 ggagcagtat ggcacgccca acaacatcga catctggatg ggcggcgtgt ccgagcctct
     2101 gaagcgcaaa ggccgcgtgg gcccactcct cgcctgcatc atcggtaccc agttcaggaa
     2161 gctccgggat ggtgatcggt tttggtggga gaacgagggt gtgttcagca tgcagcagcg
     2221 acaggccctg gcccagatct cattgccccg gatcatctgc gacaacacag gcatcaccac
     2281 cgtgtctaag aacaacatct tcatgtccaa ctcatatccc cgggactttg tcaactgcag
     2341 tacacttcct gcattgaacc tggcttcctg gagggaagcc tcctagaggc caggtaaggg
     2401 ggtgcagcag tgaggggtat atctgggctg gccagttgga accacggaga tctccttgcc
     2461 ctagatgagc ccagccctgt tctgggtgca gctgagaaaa tgagtgacta gacgttcatt
     2521 tgtgtgctca tgtatgtgcg aagtatataa attggctttt catgcgtg
//