From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: E.coli biotin carboxylase and biotin carboxyl carrier protein
Date: 29 Dec 92 00:50:12 GMT
Distribution: bionet
Checksum: 10168 10

LOCUS       ECOACOAC     4413 bp ds-DNA             BCT       04-DEC-1992
DEFINITION  E.coli biotin carboxylase and biotin carboxyl carrier protein
            (fabE) and ORF1 35 kDa protein genes, complete cds.
ACCESSION   M80458
KEYWORDS    acetyl-CoA carboxylase; biotin carboxyl carrier protein;
            biotin carboxylase.
SOURCE      Escherichia coli (strain K-12) F- DNA.
  ORGANISM  Escherichia coli
            Prokaryotae; Gram-negative facultatively anaerobic rods;
            Enterobacteriaceae.
REFERENCE   1  (sites)
  AUTHORS   Alix,J.-H.
  TITLE     A rapid procedure for cloning genes from lambda libraries by
            complementation of E.coli defective mutants: Application to the
            fabE region of the E.coli chromosome
  JOURNAL   DNA 8, 779-789 (1989)
  STANDARD  full automatic
REFERENCE   2  (sites)
  AUTHORS   Muramatsu,S. and Mizuno,T.
  TITLE     Nucleotide sequence of the fabE gene and flanking regions
            containing a bent DNA sequence of Escherichia coli
  JOURNAL   Nucleic Acids Res. 17, 3982-3982 (1989)
  STANDARD  full automatic
REFERENCE   3  (sites)
  AUTHORS   Jackowski,S. and Alix,J.-H.
  TITLE     Cloning, sequence, and expression of the pantothenate permease
            (panF) gene of Escherichia coli
  JOURNAL   J. Bacteriol. 172, 3842-3848 (1990)
  STANDARD  full automatic
REFERENCE   4  (bases 1 to 4413)
  AUTHORS   Li,S.-J. and Cronan,J.E.Jr.
  TITLE     The gene encoding the biotin carboxylase subunit of Escherichia
            coli actyl-CoA carboxylase
  JOURNAL   J. Biol. Chem. 267, 855-863 (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     mRNA            2147..4413
                     /gene="fabE"
                     /evidence="EXPERIMENTAL"
                     /citation=[4]
     CDS             492..1466
                     /function="unknown"
                     /citation=[4]
                     /note="gene not essentail for cell growth; ORF1; putative"
                     /product="35 kDa protein"
                     /codon_start=1
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRVLPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     CDS             2443..2913
                     /function="subunit of acetyl-CoA carboxylase"
                     /standard_name="BCCP"
                     /gene="fabE"
                     /evidence="EXPERIMENTAL"
                     /citation=[2]
                     /citation=[1]
                     /product="biotin carboxyl carrier protein"
                     /codon_start=1
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     CDS             2924..4273
                     /function="subunit of acetyl-CoA carboxylase"
                     /gene="fabE"
                     /evidence="EXPERIMENTAL"
                     /citation=[1]
                     /citation=[3]
                     /note="upstream of panF gene"
                     /product="biotin carboxylase"
                     /codon_start=1
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
BASE COUNT     1111 a   1097 c   1140 g   1065 t
ORIGIN      
        1 tgctttatca acggaacgct cagttcgcga aacagatcgc tggagccaac tggacgataa
       61 ctaccatttc tggccagggt ataaacctgt ttatcaccat ggagcaggtc taccgaacga
      121 tatctgccga catcattaat tcggtaattt gtggccttaa tacgctgaag tcattcgaca
      181 caaggtgggt atcaatcgcc gtcgccaccg cctgaacgcg atgactaaac ttatactgaa
      241 tggcgttgta gaaacttagc gaacagccca gcaaagtcac aaaaattgtt aacccggtga
      301 gcagcgtaac aaaggccgaa aatttcgtcg ttaatctcat ccttgtgtta actccgatag
      361 tgaggaagcg ggcatactag caaatcagat ttatctcgca atttattgcg cttcatcggc
      421 tttgcttttc cattagcgag tatagtcttc agaaattatt ttccaatcca tcatgcacat
      481 gaggaccact tatgcaggcg ttacttttag aacagcagga cggcaaaact ctcgcatcag
      541 tacagactct ggacgaaagt cgcctgccgg agggcgatgt cacggtcgat gttcactggt
      601 cgagcctgaa ctataaagat gcgctggcga ttaccggtaa gggaaaaatc atccgtaatt
      661 ttccgatgat tcctgggatc gattttgccg gaactgtacg caccagcgaa gatccgcgtt
      721 ttcatgccgg tcaggaggtg ttactcactg gctggggcgt tggtgaaaac cactggggtg
      781 ggctggcgga gcaggcgcga gtgaaaggtg actggctggt tgccatgccg caagggctgg
      841 acgcgcgtaa agcaatgatt atcggtactg ccggttttac cgccatgctg tgtgtgatgg
      901 cgctggaaga tgccggtgtt cgcccgcagg acggggagat tgtcgtgacg ggtgccagtg
      961 gtggcgtcgg cagtaccgcc gtggcgctgc tgcataagtt gggttatcag gtcgttgccg
     1021 tttccggtcg cgaaagtacc catgaatatc tgaaaagttt aggtgctagc cgtgttctcc
     1081 ctcgtgatga gtttgccgaa tcccgtcctc tggaaaaaca agtctgggct ggggcaattg
     1141 acaccgttgg cgacaaagtg ctggcaaaag tgctggcgca aatgaattac ggcggctgcg
     1201 tggcggcctg tggtctggcg ggtggtttta ctctgccaac cacggtcatg ccatttattc
     1261 tgcgtaatgt ccgtttgcaa ggggtggatt cagtaatgac gccaccagaa cgccgcgcac
     1321 aagcctggca gcgactggtc gccgatttac cggaatcatt ctatacccag gcggcaaaag
     1381 agatatctct gtcagaggca ccgaactttg ccgaggccat cattaataac cagatccagg
     1441 gtcgcacgct ggtgaaggtt aactaaccat ttagcaggga ataataagag agggaactca
     1501 ttttgaaatc attattcaat cggttaacgg gaaaagcggt tagccggaca gctttcgtcg
     1561 aacaccttgg tcaggaagtt atacaacatc atccaaactg gaaagtcatg atttcgactg
     1621 accacaaatt gatgcgcatt gatactccac taaacagcta ttattgatac gcctccgtcg
     1681 cctgttaggt ttatgttgct ttgcctgggc gcagtacgct tagcccctta cttatttctg
     1741 gtaccatggg gtgaataatc tgattttgtt tgactacaaa ttaatcactc gaacctattt
     1801 aatgctgagc attgtcaatc ggttaatttt gcgtgcttta gcattcacat ctatccagac
     1861 gatgcagtga aaattgggta atccccagca accgctgcgt aatgtcgtct atcttgtcgc
     1921 gatcctggca tccctacatt atttgtggtc tgtgaagatt atctcattgc agcccctcat
     1981 cttcgcaggg ctggcttttc agcttttcac cttacgttat aagaagttcc gtcgatgatg
     2041 gcgctaattt cgtgaattgt gcggcttgtt gcaaattaca cggtgttgaa ggttatttac
     2101 atgttagctg ttgattatct tccctgataa gaccagtatt tagctgccaa ttgctacgaa
     2161 atcgttataa tgtgcgacct cgtcctccct gacgcagttt ttgcgctgcg gaaaaggtga
     2221 cattggcgca acgaaggtat attttgtttt ttgccggagg atagcagcag atcgctgcac
     2281 aatgtccgtc aagtctaaca ttgacactct ggggcaaaat agaccggcgt cccggcctgc
     2341 tggaatttat cgctatgcat acagctgtcg gggcatacgc tttacagacg gcggtgaaac
     2401 gcctgtcaca atcacactaa acaaagagta cggaacccac tcatggatat tcgtaagatt
     2461 aaaaaactga tcgagctggt tgaagaatca ggcatctccg aactggaaat ttctgaaggc
     2521 gaagagtcag tacgcattag ccgtgcagct cctgccgcaa gtttccctgt gatgcaacaa
     2581 gcttacgctg caccaatgat gcagcagcca gctcaatcta acgcagccgc tccggcgacc
     2641 gttccttcca tggaagcgcc agcagcagcg gaaatcagtg gtcacatcgt acgttccccg
     2701 atggttggta ctttctaccg caccccaagc ccggacgcaa aagcgttcat cgaagtgggt
     2761 cagaaagtca acgtgggcga taccctgtgc atcgttgaag ccatgaaaat gatgaaccag
     2821 atcgaagcgg acaaatccgg taccgtgaaa gcaattctgg tcgaaagtgg acaaccggta
     2881 gaatttgacg agccgctggt cgtcatcgag taacgaggcg aacatgctgg ataaaattgt
     2941 tattgccaac cgcggcgaga ttgcattgcg tattcttcgt gcctgtaaag aactgggcat
     3001 caagactgtc gctgtgcact ccagcgcgga tcgcgatcta aaacacgtat tactggcaga
     3061 tgaaacggtc tgtattggcc ctgctccgtc agtaaaaagt tatctgaaca tcccggcaat
     3121 catcagcgcc gctgaaatca ccggcgcagt agcaatccat ccgggttacg gcttcctctc
     3181 cgagaacgcc aactttgccg agcaggttga acgctccggc tttatcttca ttggcccgaa
     3241 agcagaaacc attcgcctga tgggcgacaa agtatccgca atcgcggcga tgaaaaaagc
     3301 gggcgtccct tgcgtaccgg gttctgacgg cccgctgggc gacgatatgg ataaaaaccg
     3361 tgccattgct aaacgcattg gttatccggt gattatcaaa gcctccggcg gcggcggcgg
     3421 tcgcggtatg cgcgtagtgc gcggcgacgc tgaactggca caatccatct ccatgacccg
     3481 tgcggaagcg aaagctgctt tcagcaacga tatggtttac atggagaaat acctggaaaa
     3541 tcctcgccac gtcgagattc aggtactggc tgacggtcag ggcaacgcta tctatctggc
     3601 ggaacgtgac tgctccatgc aacgccgcca ccagaaagtg gtcgaagaag cgccagcacc
     3661 gggcattacc ccggaactgc gtcgctacat cggcgaacgt tgcgctaaag cgtgtgttga
     3721 tatcggctat cgcggtgcag gtactttcga gttcctgttc gaaaacggcg agttctattt
     3781 catcgaaatg aacacccgta ttcaggtaga acacccggtt acagaaatga tcaccggcgt
     3841 tgacctgatc aaagaacagc tgcgtatcgc tgccggtcaa ccgctgtcga tcaagcaaga
     3901 agaagttcac gttcgcggcc atgcggtgga atgtcgtatc aacgccgaag atccgaacac
     3961 cttcctgcca agtccgggca aaatcacccg tttccacgca cctggcggtt ttggcgtacg
     4021 ttgggagtct catatctacg cgggctacac cgtaccgccg tactatgact caatgatcgg
     4081 taagctgatt tgctacggtg aaaaccgtga cgtggcgatt gcccgcatga agaatgcgct
     4141 gcaggagctg atcatcgacg gtatcaaaac caacgttgat ctgcagatcc gcatcatgaa
     4201 tgacgagaac ttccagcatg gtggcactaa catccactat ctggagaaaa aactcggtct
     4261 tcaggaaaaa taagactgct aaagcgtcaa aagcggattt tccggccttt tttattactg
     4321 gggatcgaca acccccataa ggtacaatcc ccgctttctt cacccatcag ggacaaaaaa
     4381 tggacactcg ttttgttcag gcccataaag agg
//