From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Caldocellum saccharolyticum beta-mannanase/endoglucanase (manA)
Date: 26 Jan 93 05:46:35 GMT
Distribution: bionet
Checksum: 39933 10

LOCUS       CDCMANA      4977 bp ds-DNA             BCT       25-JAN-1993
DEFINITION  Caldocellum saccharolyticum beta-mannanase/endoglucanase (manA)
            gene, complete cds.
ACCESSION   L01257
KEYWORDS    beta-mannanase; endo-1,4 beta-mannanase; endoglucanase; manA gene.
SOURCE      Caldocellum saccharolyticum (library: lambda NZP2) DNA.
  ORGANISM  Caldocellum saccharolyticum
            Prokaryotae.
REFERENCE   1  (bases 1 to 4977)
  AUTHORS   Gibbs,M.D., Saul,D.J., Luthi,E. and Bergquist,P.L.
  TITLE     The beta-mannanase from Caldocellum saccharolyticum is part of a
            multidomain enzyme
  JOURNAL   Appl. Environ. Microbiol. 58, 3864-3867 (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     CDS             841..4836
                     /EC_number="3.2.1.78"
                     /gene="manA"
                     /note="endoglucanase EC_Number= 3.2.1.4"
                     /product="beta-mannanase"
                     /codon_start=1
                     /translation="MRLKTKIRKKWLSVLCTVVFLLNILFIANVTILPKVGAATSNDG
                     VVKIDTSTLIGTNHAHCWYRDRLDTALRGIRSWGMNSVRVVLSNGYRWTKIPASEVAN
                     IISLSRSLGFKAIILEVHDTTGYGEDGAACSLAQAVEYWKEIKSVLDGNEDFVIINIG
                     NEPYGNNNYQNWVNDTKNAIKALRDAGFKHTIMVDAPNWGQDWSNTMRDNAQSIMEAD
                     PLRNLVFSIHMYGVYNTASKVEEYIKSFVDKGLPLVIGEFGHQHTDGDPDEEAIVRYA
                     KQYKIGLFSWSWCGNSSYVGYLDMVNNWDPNNPTPWGQWYKTNAIGTSSTPTPTSTVT
                     PTPTPTPTPTPTVTATPTPTPTPVSTPATSGQIKVLYANKETNSTTNTIRPWLKVVNS
                     GSSSIDLSRVTIRYWYTVDGERAQSAISDWAQIGASNVTFKFVKLSSSVSGADYYLEI
                     GFKSGAGQLQPGKDTGEIQMRFNKDDWSNYNQGNDWSWIQSMTSYGENEKVTAYIDGV
                     LVWGQEPSGATPAPAPTATPTPTPTVTPTPTVTPTPTVTATPTPTPTPTPTPVSTPAT
                     GGQIKVLYANKETNSTTNTIRPWLKVVNSGSSSIDLSRVTIRYWYTVDGERAQSAISD
                     WAQIGASNVTFKFVKLSSSVSGADYYLEIGFKSGAGQLQPGKDTGEIQIRFNKSDWSN
                     YNQGNDWSWIQSMTSYGENEKVTAYIDGVLVWGQEPSGTTPSPTSTPTVTVTPTPTPT
                     PTPTPTPTVTPTPTVTPTPTVTATPTPTPTPIPTVTPLPTISPSPSVVEITINTNAGR
                     TQISPYIYGANQDIEGVVHSARRLGGNRLTGYNWENNFSNAGNDWYHSSDDYLCWSMG
                     ISGEDAKVPAAVVSKFHEYSLKNNAYSAVTLQMAGYVSKDNYGTVSENETAPSNRWAE
                     VKFKKDAPLSLNPDLNDNFVYMDEFINYLINKYGMASSPTGIKGYILDNEPDLWASTH
                     PRIHPNKVTCKELIEKSVELAKVIKTLDPSAEVFGYASYGFMGYYSLQDAPDWNQVKG
                     EHRWFISWYLEQMKKASDSFGKRLLDVLDLHWYPEARGGNIRVCFDGENDTSKEVVIA
                     RMQAPRTLWDPTYKTSVKGQITAGENSWINQWFSDYLPIIPNVKADIEKYYPGTKLAI
                     SEFDYGGRNHISGGIALADVLGIFGKYGVNFAARWGDSGSYAAAAYNIYLNYDGKGSK
                     YGNTNVSANTSDVENMPVYASINGQDDSELHIILINRNYDQKLQVKINITSTPKYTKA
                     EIYGFDSNSPEYKKMGNIDNIESNVFTLEVPKFNGVSHSITLDFNVSIKIIQNEVIKF
                     IRNLVFMRALV"
BASE COUNT     1698 a    815 c   1265 g   1199 t
ORIGIN      
        1 gggtagcaat acatggtttg gattccaggc atggtcgatg cagagggtag cggagtatta
       61 ctatgtgaca ggagataaag atgcaggggc actgcttgag aagtgggtaa gctggataaa
      121 gagtgtagtg aagttgaata gtgatggtac atttgcgata ccatcgacgc ttgattggag
      181 tgggcagcca gacacatgga atgggacata tacaggtaat ccgaacttgc atgtgaaggt
      241 agtagattat gggacggatt taggaataac ggcatcactt gcgaatgcac tactttatta
      301 cagtgcaggg acgaagaagt atggggtatt tgatgaggaa gcgaagaatt tagcgaagga
      361 attgctggac aggatgtgga agttatacag ggatgagaaa ggtttatcgg cgccagagaa
      421 gagagcggac tacaagaggt tctttgagca agaggtatac attccggcag gatggacagg
      481 gaagatgccg aatggagatg taataaagag cggagttaag tttatagaca taaggagcaa
      541 gtacaaacaa gatcctgatt ggccgaagtt agaggcggca tacaagtcag ggcaggtacc
      601 ggagttcaga tatcacaggt tctgggcaca gtgtgacata gcaattgtta atgcaacata
      661 tgaaattctg ttcggtaatc aataatgagt aggtaaatgg aaatttagcg gggtggcaca
      721 tctataagtt tggtgtgctg cctcgctaaa atcctgtatg gaagtgttcg aaaaatagta
      781 caaaaaaatg gcgaggtaaa atggcaaaaa tgcataaaaa agagggggtt aggttttaag
      841 atgagactaa aaacaaaaat aagaaagaaa tggttgagtg tactatgtac agttgttttt
      901 ttattgaaca ttttgtttat agcaaatgta acgattttac ccaaagttgg tgcggctaca
      961 tctaatgatg gagtagtgaa gatagatacg agtacgttaa taggaaccaa tcacgcacat
     1021 tgctggtaca gagatagact tgatacggca ttgcgtggaa ttaggtcatg gggtatgaac
     1081 tctgtgaggg tagtgctgag taatggttac cgatggacga agataccagc aagtgaagtg
     1141 gcaaatatta tatcattgtc aagaagtctt ggtttcaaag ctattatatt agaagtgcac
     1201 gacacaacag gatatggaga agatggggca gcatgttcat tggcacaagc agtggaatat
     1261 tggaaggaga taaagagcgt attagacggt aacgaagatt ttgtaattat aaacattggt
     1321 aatgagccgt atgggaacaa taactatcaa aactgggtta atgacacgaa gaacgctata
     1381 aaagcactta gagatgcagg attcaagcac acgataatgg tggatgcgcc gaactggggt
     1441 caggattggt ctaatactat gagagataat gcccagagca taatggaagc agatccgctg
     1501 cgcaatttgg tattttcgat tcatatgtat ggcgtataca atacagcaag caaggtcgaa
     1561 gagtacatca aatcatttgt tgataagggg ttaccattgg ttattggaga atttggacat
     1621 cagcacacag atggtgaccc tgatgaagaa gctattgtca ggtatgcaaa acagtacaag
     1681 ataggattat ttagttggtc gtggtgtgga aattcgagct atgttgggta tttggacatg
     1741 gtaaacaact gggaccccaa taatccaact ccatggggac aatggtataa aactaatgca
     1801 attggtacat cttctacacc aacacctacg tcgacagtaa cgccaacgcc cacccccacg
     1861 ccaacaccaa caccgacagt aacagcgaca ccaacaccaa cacctacacc tgttagcaca
     1921 cctgcgacaa gtgggcagat aaaggtactg tatgctaaca aggagacaaa cagcactaca
     1981 aacacgataa ggccgtggtt gaaggtagtg aatagtggta gcagtagcat agatttgagc
     2041 agggtaacga taaggtactg gtacacggta gatggtgaga gggcacagag tgcgatatca
     2101 gactgggcac agataggagc aagcaatgta acattcaagt ttgtgaagct gagcagtagt
     2161 gtaagtggag cggactatta cttggagata ggatttaaga gtggagcagg gcagttacag
     2221 cctgggaagg atacaggaga gatacagatg aggtttaaca aggatgactg gagcaattac
     2281 aatcagggga atgactggtc atggatacag agcatgacga gttatggaga gaatgagaag
     2341 gtaacagcgt atatagatgg tgtgctggta tggggacagg agccgagtgg agcgacacct
     2401 gcgccagccc cgacagcgac gccgacacct acaccaacag taacaccgac gccgacagtg
     2461 acacctacgc cgacagtaac agcaaccccg actccaacac cgaccccgac gccaacacct
     2521 gttagcacac ctgcgacagg tgggcagata aaggtactgt atgctaacaa ggagacaaac
     2581 agcacgacaa acacgataag gccatggttg aaggtagtga acagtggtag cagtagcata
     2641 gatttgagca gggtaacgat aaggtactgg tacacggtag atggtgagag ggcacagagt
     2701 gcgatatcag actgggcaca gataggagca agcaatgtaa cattcaagtt tgtgaagctg
     2761 agcagtagtg taagtggagc ggactattac ttggagatag gatttaagag tggagcaggg
     2821 cagttgcagc ctgggaagga cacaggagag atacagataa gatttaataa gagtgactgg
     2881 agcaattaca atcaggggaa tgactggtca tggatacaga gcatgacgag ttatggagag
     2941 aatgagaagg taacggcgta tatagatggt gtgctggtat ggggacagga gccgagtgga
     3001 acaacacctt cgccgacgtc aacaccgaca gtaacggtaa caccaacacc gacacccaca
     3061 ccaacaccta cacctacacc aacagtaaca ccgacaccga cagttacacc tacgccgaca
     3121 gtaacagcaa cgccaacccc gacaccgaca cctataccaa cagtaacacc attaccgaca
     3181 atatcgccat ctccatctgt tgttgaaatt acaataaata caaatgcagg aagaacgcaa
     3241 attagcccgt atatatatgg tgcaaaccaa gatattgaag gggttgtgca ctcagcaaga
     3301 agattaggtg gaaatagact aacaggatat aattgggaaa acaatttctc aaatgcaggg
     3361 aacgattggt atcattcaag tgatgactat ttgtgctgga gtatggggat ttctggtgaa
     3421 gatgcaaagg ttccagcggc agtggtatct aaatttcatg agtattccct taaaaataat
     3481 gcttattctg ctgtaacttt gcaaatggca ggatatgtgt caaaagataa ttatggcaca
     3541 gttagtgaaa atgaaacagc accatctaat aggtgggcag aggtgaaatt taagaaagat
     3601 gctcctctat ctttgaatcc agacttgaat gataactttg tttatatgga tgagttcata
     3661 aattatttga taaacaaata cggaatggct tcttcaccta ccgggataaa aggatatata
     3721 cttgataatg agcctgattt gtgggcatca acacatccac gtatacatcc taataaagtc
     3781 acatgcaaag agttgattga aaaatctgtt gaactggcaa aagttataaa aacccttgat
     3841 ccttcagctg aagtttttgg atatgcatca tacgggttta tgggttatta tagtcttcaa
     3901 gatgcacctg attggaatca agttaaaggt gaacacagat ggtttattag ctggtatctt
     3961 gaacagatga aaaaggcatc agacagtttt ggtaaaagac tattagatgt gcttgatcta
     4021 cactggtatc ccgaggcaag gggtggaaat attcgcgtat gctttgatgg tgaaaatgac
     4081 acatcaaaag aagttgttat agctaggatg caggctccga gaacactatg ggatccgacc
     4141 tataaaacat cagtgaaagg acaaattaca gctggtgaga acagctggat aaaccagtgg
     4201 ttttcagatt atttgcctat aattccaaac gtaaaagcgg acatagagaa atattatcct
     4261 ggtacaaaac ttgctattag cgaattcgac tatggcggtc gaaatcatat ttcaggtggt
     4321 attgcattag ctgatgttct tggtatattt ggtaaatatg gagtgaactt tgcggcaaga
     4381 tggggcgatt ctggtagtta tgcagcagct gcatataaca tttatcttaa ttatgatgga
     4441 aaaggctcaa aatatggcaa tacaaatgta agtgcaaata cgagtgatgt tgaaaatatg
     4501 ccagtttatg cctcaataaa tggacaggat gattctgaac ttcatattat actaataaac
     4561 agaaactatg atcaaaaatt acaagtcaaa ataaacataa caagcacgcc gaaatataca
     4621 aaagcggaaa tatatggatt tgacagtaac agcccagaat ataagaaaat gggaaatata
     4681 gataatattg agagcaatgt atttacactt gaggttccca aatttaacgg tgtatcacat
     4741 agtattacgt tagatttcaa tgtaagcata aaaattatac aaaatgaggt tataaaattc
     4801 ataaggaatt tggtttttat gagggctctg gtgtaatgcc aaagtggcaa ttgataatgt
     4861 aacttggacg taagaattgt gtcaaatatg ttgatagtat ttttgcaaca gaggttgaaa
     4921 tattttcaaa atgagcattg ttaatttaaa tagactgaga gcacaaatgt gaagctt
//