From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Pseudomonus aeruginosa lipA and lipB gene for lipase and it's
Date: 9 Jan 93 05:47:34 GMT
Distribution: bionet
Checksum: 34176 6

LOCUS       PSELIPAB     2655 bp ds-DNA   Circular  BCT       09-JAN-1993
DEFINITION  Pseudomonus aeruginosa lipA and lipB gene for lipase and it's
            modulator, complete cds.
ACCESSION   D10048
KEYWORDS    lipase.
SOURCE      Pseudomonas aeruginosa DNA.
  ORGANISM  Pseudomonas aeruginosa
            Prokaryotae; Bacteria; Gracilicutes; Scotobacteria;
            Pseudomonadaceae.
REFERENCE   1  (bases 1 to 2655)
  AUTHORS   Chihara-Siomi,M., Yoshikawa,K., Oshima-Hirayama,N., Yamamoto,K.,
            Sogabe,Y., Nakatani,T., Nishioka,T. and Oda,J.
  TITLE     Purification, molecular cloning, and expression of lipase from
            Pseudomonas aeruginosa
  JOURNAL   Arch. Biochem. Biophys. 296, 505-513 (1992)
  STANDARD  full automatic
COMMENT     Submitted (06-Nov-1991) to DDBJ by: Kazuhiro Yoshikawa Institute
            for Chemical Research Kyoto University Uji, Kyoto 611 Japan Phone:
            0774-32-3111 Fax:    0774-33-1247.
FEATURES             Location/Qualifiers
     RBS             295..298
     sig_peptide     308..385
                     /codon_start=1
     mat_peptide     386..1240
                     /EC_number="3.1.1.3"
                     /product="lipase mature peptide"
                     /codon_start=1
     RBS             1280..1283
     CDS             308..1243
                     /EC_number="3.1.1.3"
                     /gene="lipA"
                     /product="lipase precursor"
                     /codon_start=1
                     /translation="MKKKSLLPLGLAIGLASLAASPLIQASTYTQTKYPIVLAHGMLG
                     FDNILGVDYWFGIPSALRRDGAQVYVTEVSQLDTSEVRGEQLLQQVEEIVALSGQPKV
                     NLIGHSHGGPTIRYVAAVRPDLIASATSVGAPHKGSDTADFLRQIPPGSAGEAILSGL
                     VNSLGALISFLSSGSTGTQNSLGSLESLNSEGAARFNAKYPHGVPTSACGEGAYKVNG
                     VSYYSWSGSSPLTNFLDPSDAFLGASSLTFKNGTANDGLVGTCSSHLGMVIRDNYRMN
                     HLDEVNQVFGLTSLFETSPVSVYRQHANRLKNASL"
     CDS             1293..2312
                     /gene="lipB"
                     /product="proposal modulator or activator protein for
                     lipase"
                     /codon_start=1
                     /translation="VKKILLLIPLAFAASLAWFVWLEPSPAPETAPPASPQAGADRAP
                     PAASAGEAVPAPQVMPAKVAPLPTSFRGTSVDGSFSVDASGNLLITRDIRNLFDYFLS
                     DGEEPLQQSLDGLRAYIAAELQEPARGQALALMQQYIDYKKELVLLERDLPRLADLDA
                     LRQREAAVKALRARIFSNEAHVAFFADEETYNQFTLERLAIRQDGKLSTEEKAAAIDR
                     LRASLPEDQQESVLPQLQSELQQQTAALQAAGAGPEAIRQMRQQLVGAEATTRLEQLD
                     RQRSAWKGRLDDYFAEKSRIEGNAGLSEADRRAAVERLAEERFSEQERLRLGALEQMR
                     QAEQR"
BASE COUNT      496 a    961 c    815 g    383 t
ORIGIN      
        1 gtcgaccatt tcagcctgtt ttgctcgcaa aacgacgccg cgggcgtgcg caccgcacac
       61 tcggtcgctg ggcgttgtgc ggggaagatt caaacgagcg tttcgcgccg taacaacccg
      121 cctcttccgc tctgccacgc aggttatgac cggccgccag gaagccgcgg atttcctggc
      181 ctggaggaaa aaagccgaag ctggcacggt tcctgcgcaa gggacagcga agcggttctc
      241 ccggaaggat tcgggcgatg gctggcagga cgcgcccctc ggccccatca acctgagatg
      301 agacaacatg aagaagaagt ctctgctccc cctcggcctg gccatcggcc tcgcctctct
      361 cgctgccagc cctctgatcc aggccagcac ctacacccag accaaatacc ccatcgtgct
      421 ggcccacggc atgctcggct tcgacaacat cctcggggtc gactactggt tcggcattcc
      481 cagcgccttg cgccgtgacg gtgcccaggt ctacgtcacc gaagtcagcc agttggacac
      541 ctcggaagtc cgcggcgagc agttgctgca acaggtggag gaaatcgtcg ccctcagcgg
      601 ccagcccaag gtcaacctga tcggccacag ccacggcggg ccgaccatcc gctacgtcgc
      661 cgccgtacgt cccgacctga tcgcttccgc caccagcgtc ggcgccccgc acaagggttc
      721 ggacaccgcc gacttcctgc gccagatccc accgggttcg gccggcgagg caatcctctc
      781 cgggctggtc aacagcctcg gcgcgctgat cagcttcctt tccagcggca gcaccggtac
      841 gcagaattca ctgggctcgc tggagtcgct gaacagcgag ggggccgcgc gcttcaacgc
      901 caagtacccg catggcgtcc ccacctcggc ctgcggcgag ggcgcctaca aggtcaacgg
      961 cgtgagctat tactcctgga gcggttcctc gccgctgacc aacttcctcg atccgagcga
     1021 cgccttcctc ggcgcctcgt cgctgacctt caagaacggc accgccaacg acggcctggt
     1081 cggcacctgc agttcgcacc tgggcatggt gatccgcgac aactaccgga tgaaccacct
     1141 ggacgaggtg aaccaggtct tcggcctcac cagcctgttc gagaccagcc cggtcagcgt
     1201 ctaccgccag cacgccaacc gcctgaagaa cgccagcctg taggaccccg gccggggcct
     1261 cggccccggc cctttcccgg aagccccctc gcgtgaagaa aatcctcctg ctgattccac
     1321 tggcgttcgc cgccagcctg gcctggttcg tctggctgga accttccccc gcccccgaga
     1381 cggcgccccc ggccagcccg caggcgggcg cagaccgcgc cccgccagca gcctccgcgg
     1441 gagaagcggt gccggccccc caggtcatgc cggccaaggt cgcgccgctg ccaacctcct
     1501 tcaggggcac cagcgtcgat ggcagtttca gtgtcgacgc cagcggcaac ctgctgatca
     1561 cccgcgacat ccgcaacctg ttcgactact tcctcagcga cggcgaagag cccctgcagc
     1621 aaagcctgga cggcctgcgc gcctacatcg ccgccgaact ccaggagccg gcgcgcggcc
     1681 aggcgttggc gctgatgcag caatacatcg actacaagaa ggaactggtg ctgctcgaac
     1741 gcgacctgcc gcgcctggcc gacctcgacg ccctgcgcca gcgggaagcc gcggtgaaag
     1801 ccctgcgcgc gcggatcttc agcaacgaag cgcacgtggc gttcttcgcc gacgaggaaa
     1861 cctacaacca gttcaccctg gagcgcctgg cgatccgcca ggatggcaag ctcagcaccg
     1921 aggaaaaggc cgccgccatc gaccgcctgc gcgccagcct gccggaagac cagcaggaaa
     1981 gcgtgctgcc gcaactgcaa agcgaactgc agcagcagac cgccgccctc caggccgctg
     2041 gcgccggccc ggaagccatc cgccagatgc gtcagcaact ggtgggcgcc gaagccacca
     2101 cccgcctgga gcaactcgat cggcaacgct cggcctggaa gggccggctg gacgactatt
     2161 tcgccgagaa gagccggatc gaaggcaatg ccgggctgag cgaagccgac cgccgcgcgg
     2221 cggtcgaacg cctggccgag gagcgcttca gcgaacagga acgcttgcgc ctgggcgcgc
     2281 tggaacagat gcgccaggcc gagcagcgct gaccggcacg gaaacgccga gaacgcggcg
     2341 aagggcgctt cggcggataa cgctaccctc aggggtgcag ccctggcgtg gccggcgagg
     2401 cggaaacctg tgctgcgcgc cgcaacgaaa aagggcggcc acccgaaggt gtccgccctt
     2461 tttcgtcgcc agcccggttc agcgggacag cttgccgtcc agcgagaact tgccggcgcc
     2521 atcgatcagc agcgccacgc tgatcatcag cagggtcagg gcatattcat agccgttgtc
     2581 ggtgatgaag aagccattgc cgatgtgcac gctgaagatc gccacgatcc gttgacctgc
     2641 aggtcgaccc agatc
//