From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Bacillus subtilis antiterminator regulatory protein (glpP),
Date: 22 Aug 92 10:00:21 GMT
Distribution: bionet
Checksum: 54317     6

LOCUS       BACGLPPFK    1980 bp ds-DNA             BCT       22-AUG-1992
DEFINITION  Bacillus subtilis antiterminator regulatory protein (glpP),
            glycerol uptake facilitator (glpF) genes, complete cds, glycerol
            kinase (glpK) gene, 5' end.
ACCESSION   M99611
KEYWORDS    antiterminator; glpF gene; glpK gene; glpP gene; glycerol kinase;
            glycerol uptake facilitator; regulatory protein.
SOURCE      Bacillus subtilis DNA.
  ORGANISM  Bacillus subtilis
            Prokaryotae; Firmicutes; Endospore-forming rods and cocci;
            Bacillaceae.
REFERENCE   1  (bases 1 to 1980)
  AUTHORS   Beijer,L., Nilsson,R.-P., Holmberg,C. and Rutberg,L.
  TITLE     Nucleotide sequence of the Bacillus subtilis glpP gene encoding a
            regulatory protein and the glpF gene encoding the glycerol uptake
            facilitator and evidence for a glpFK operon
  JOURNAL   Unpublished (1992)
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     RBS             318..324
                     /gene="glpP"
                     /note="putative"
                     /citation=[1]
     CDS             328..906
                     /function="putative antiterminator"
                     /product="regulatory protein"
                     /gene="glpP"
                     /evidence=EXPERIMENTAL
                     /codon_start=1
                     /translation="MMSFHNQPILPAIRNMKQFDEFLNSSFSYGVILDIHLGQLKGVI
                     KEAQKHGKNMMVHVDLIQGIKHDEYGAEFICQDIKPAGIISTRSNVIAKAKQKKIYAI
                     QRLFLLDTSAMEKSMEFIGKHKPDFIEVLPGIVPSLIQEIKEKTGIPIFAGGFIRTEE
                     DVEQALKAGAVAVTTSNTKLWKKYENFLTESD"
                     /citation=[1]
     RBS             1071..1076
                     /gene="glpF"
                     /note="putative"
                     /citation=[1]
     CDS             1085..1909
                     /product="glycerol uptake facilitator"
                     /gene="glpF"
                     /note="putative"
                     /codon_start=1
                     /translation="MTAFWGEVIGTMLLIIFGAGVCAGVNLKKSLSFQSGWIVVVFGW
                     GLGVAMAAYAVGGISGAHLNPALTIALAFVGDFPWKEVPVYIAAQMIGAIIGAVIIYL
                     HYLPHWKSTDDPAAKLGVFSTGPSIPHTFANVLSEVIGTFVLVLGILAIGANQFTEGL
                     NPLIVGFLIVAIGISLGGTTGYAINPARDLGPRIAHAFLPIPGKGSSNWKYAWVPVVG
                     PILGGSFGGVFYNAAFKGHITSSFWIVSVILVVVLLGLYVYTKSHSAKTLSNSKYI"
                     /citation=[1]
     RBS             1912..1920
                     /gene="glpK"
                     /note="putative"
                     /citation=[1]
     CDS             1928..1980
                     /product="glycerol kinase"
                     /gene="glpK"
                     /evidence=EXPERIMENTAL
                     /codon_start=1
                     /translation="METYILSLDQGTTSSRA"
                     /citation=[1]
                     /partial
BASE COUNT      538 a    397 c    494 g    551 t
ORIGIN
        1 aagcttttag gtgagcttca agctctgaga gaacacccgg cagtcgggga tgttagagga
       61 aaagggctgc tgatcggaat cgaactcgtc aaagacaaat tgactaaaga gccggctgat
      121 gccgccaaag taaaccaagt ggttgcggcg tgcaaagaaa aagggctgat catcggcaaa
      181 aacggcgata cagtcgccgg ctacaacaat gtcatccacg ttgcgccgcc attttgcctg
      241 acagaagagg acctttcctt tatcgtgaaa acggtgaaag aaagctttca aacgatataa
      301 tataaaagtg aattattaaa ggagcacatg atgagttttc acaaccagcc gatcttaccg
      361 gctattcgca atatgaagca atttgatgag tttttgaaca gttcattctc ttacggtgtt
      421 attcttgata ttcatctcgg ccagctgaag ggcgtgatca aagaagcgca aaagcacgga
      481 aagaacatga tggtgcacgt cgatctcatt caagggatta agcatgacga atacggtgcg
      541 gaatttatat gtcaggatat caagcccgct gggattattt ccacaagatc gaatgtgatt
      601 gccaaagcga agcagaagaa aatttatgcc attcagcgcc tgtttttgct tgatacaagc
      661 gcgatggaga aaagcatgga gtttatcggc aagcataagc ctgacttcat cgaagtgctt
      721 cccggcatcg ttccgtcact cattcaggaa ataaaagaga aaacagggat tcccatcttt
      781 gccgggggtt tcatccgtac ggaagaggat gtagagcagg cattgaaagc gggggctgta
      841 gctgtcacaa catctaatac caaattgtgg aaaaaatatg aaaacttttt gacggaaagt
      901 gattgacacc gctttcatgc actgatacaa ttgcactagg ttaatacatt gtgatggaga
      961 actcggagac cacagcagct ctttacggca aatgtttatg cacccgtaaa gcggtttgtt
     1021 gtggtttttt tattctcttc ttctctatca tgctttttaa tcgtgacttt aggaggaatg
     1081 tgctatgaca gcattttggg gagaagtcat cggtacgatg ctgcttatca tttttggtgc
     1141 aggtgtttgt gcaggtgtca atttaaagaa atcgctttca ttccagtctg gctggattgt
     1201 tgttgtattt ggatggggat tgggtgttgc catggcggca tacgcggttg gcggcatcag
     1261 cggtgcccat ttgaatccgg cgctaacgat agcgcttgca tttgtaggag attttccttg
     1321 gaaagaggtt ccggtttata ttgcggcgca aatgatcgga gcgatcatcg gtgcggtgat
     1381 tatttatctg cattacctcc cgcactggaa gtcaacggat gatcccgctg ccaagctggg
     1441 tgttttctca acagggccca gcattccgca tacatttgca aacgttttaa gcgaagtgat
     1501 tgggacattt gtccttgtac ttggaatctt ggccataggt gcaaatcaat ttacagaagg
     1561 acttaatcct ttaatcgtcg gtttcctcat tgtagcaatc ggtatttctc tgggaggcac
     1621 caccggctat gctatcaatc ctgcacgtga cttaggtccg cggatcgccc acgcttttct
     1681 tccgattccg gggaagggct catcaaactg gaaatacgca tgggttccgg tagtcggccc
     1741 gattttaggc ggatcattcg gcggtgtatt ttacaacgct gcatttaaag ggcacatcac
     1801 aagcagcttc tggattgtaa gcgttatatt ggttgtggta ttgttaggac tctatgttta
     1861 tacgaaatca cattctgcta aaacattatc aaattctaaa tatatttaat caaaggggag
     1921 acatcttatg gaaacgtaca ttttatcctt agatcagggg acgacaagtt caagagcgat
//