From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Saccharomyces cerevisiae COT1 protein gene, complete cds.
Date: 4 Sep 92 10:07:32 GMT
Distribution: bionet
Checksum: 21592     6

LOCUS       YSCCOT1A     2675 bp ds-DNA             PLN       04-SEP-1992
DEFINITION  Saccharomyces cerevisiae COT1 protein gene, complete cds.
ACCESSION   M88252
KEYWORDS    COT1 protein; transmembrane protein.
SOURCE      Saccharomyces cerevisiae (library: YEp24) DNA.
  ORGANISM  Saccharomyces cerevisiae
            Eukaryota; Fungi; Eumycota; Hemiascomycetes; Endomycetales;
            Saccharomycetaceae.
REFERENCE   1  (bases 1 to 2675)
  AUTHORS   Conklin,D.S., McMaster,J.A., Culbertson,M.R. and Kung,C.
  TITLE     COT1, a gene involved in cobalt accumulation in Saccharomyces
            cerevisiae
  JOURNAL   Mol. Cell. Biol. 12, 3678-3688 (1992)
  STANDARD  full staff_entry
FEATURES             Location/Qualifiers
     misc_feature    28..75
                     /note="hydrophobic domain"
                     /gene="COT1"
     misc_feature    139..180
                     /note="hydrophobic domain"
                     /gene="COT1"
     misc_feature    232..300
                     /note="hydrophobic domain"
                     /gene="COT1"
     misc_feature    340..399
                     /note="hydrophobic domain"
                     /gene="COT1"
     misc_feature    418..444
                     /note="potential metal coordination"
                     /gene="COT1"
                     /note="putative"
     misc_feature    487..507
                     /note="potential metal coordination"
                     /gene="COT1"
                     /note="putative"
     misc_feature    730..795
                     /note="hydrophobic domain"
                     /gene="COT1"
     misc_feature    835..885
                     /note="hydrophobic domain"
                     /gene="COT1"
     TATA_signal     872..875
                     /gene="COT1"
     TATA_signal     959..962
                     /gene="COT1"
     CDS             1003..2322
                     /product="COT1 protein"
                     /gene="COT1"
                     /codon_start=1
                     /translation="MKLGSKQVKIISLLLLDTVFFGIEITTGYLSHSLALIADSFHML
                     NDIISLVVALWAVNVAKNRNPDSTYTYGWKRAEILGALINAVFLIALCVSILIEALQR
                     IIAPPVIENPKFVLYVGVAGLISNTVGLFLFHDNDQEHGHGHGHSHGGIFADHEMHMP
                     SSHTHTHAHVDGIENTTPMDSTDNISEIMPNAIVDSFMNENTRLLTPENASKTPSYST
                     SSHTIASGENYTEHNKRKRSLNMHGVFLHVLGDALGNIGVMLSAFFIWKTDYSWKYYT
                     DPLVSLIITGIIFSSALPLSCKASKILLQATPSTLSGDQVEGDLLKIPGIIAIHDFRV
                     WNLTESIFIASLHIQLDISPEQFTDLAKIVRSKLHRYGIHSATLQPEFITREVTSTER
                     AGDSQGDHLQNDPLSLRPKTYGTGISGSTCLVDDAANCNTADCLEDH"
     terminator      2411..2428
                     /gene="COT1"
BASE COUNT      849 a    521 c    490 g    815 t
ORIGIN
        1 ggactttcac cgcgcttaga tcaagagggc atcatgaggc tacctacaat tgacatacta
       61 agtcaagaaa aataatagtg tatcgaatac gcgtggtagc tacattaaat actaaagtat
      121 ggtttttttt tttaagtagg tttactcttc atattcttca ttttcatttt tccttttctt
      181 cccgttcttc atttttattc ctagaaaaaa aaataaatat gtctatcgtt aacctaaaca
      241 taaacaaagt ttagcccgct tatttaaatg atgcccagta ctttttttca aaactcatat
      301 aattatatat atcaagaatg ctttccaacc accgcttgta ttaaggtgta gaaaggtggt
      361 ttttgtattc aaaaagagta caagcccatt atagctaact taacagtcca ccctactata
      421 tcccacgcat atctggtttt aacttgtggc gggcgatatg gaacgaattt ttcaaagtta
      481 accatagaaa aaagcatgtg atcaaggcag agtgaacgag ggaaaaagaa agtgtggaga
      541 aggccacctt ttgaaaagtt taccaatcga tcggtctctc agcactttct acatttaatc
      601 tctttatagt gaacatcgct ggctcatgct ggttcccatg cataactcac ccacggccgc
      661 caacggcctt atcacttact gtcgccagct ccggtcttag aaagggtaaa aaaaaccggg
      721 tgtatactat acactcatac atccgctcac ccgtttccag tagtgaattt tcattttctg
      781 tacgaaggca atataagctg acgatccgca taaacgagaa gactcatttg taatcgagaa
      841 tatcgtacaa taaaggaatt tattggtctt atatattcta cgtgggagct cgaaaagcat
      901 ttctggttct atttagtaac caattatttt cactttgctc agaatagttc tgcatagcta
      961 tagaaagaaa gttaacacaa agtacggaaa gattgagtaa atatgaaact cggaagcaaa
     1021 caggtaaaaa ttatatcctt gttgctgcta gacacagtgt tcttcgggat cgagataact
     1081 accgggtact tgtctcactc tttggctcta atcgcggact cattccatat gctaaacgat
     1141 ataatttctc ttgtggttgc actttgggcc gtaaatgttg ccaaaaacag aaatccggat
     1201 tcaacgtaca cttatggttg gaaaagggcg gagattttgg gtgctctgat taacgccgtc
     1261 tttttgattg ccttatgtgt ctcaattttg atagaagcgc tacaaagaat tattgctccc
     1321 cccgtgattg aaaatcctaa gtttgtgttg tatgtgggtg tcgcagggtt gatatcgaac
     1381 accgttggac ttttcttatt tcacgacaat gatcaagagc atggacatgg acacggacat
     1441 tcccatggcg gtatctttgc cgaccatgag atgcatatgc catcatccca cacacataca
     1501 catgcccatg ttgatggaat agagaatact acaccaatgg atagtacgga taacattagt
     1561 gagattatgc ctaatgctat agtagatagt tttatgaacg aaaatactag attattgaca
     1621 ccggaaaatg catccaagac gccatcatac tcaacgtcaa gccatacgat tgccagcggc
     1681 gaaaattaca cagaacacaa caagcgcaag agatctttaa atatgcatgg tgtgtttctt
     1741 cacgttttgg gcgatgctct tggcaacatc ggcgttatgt tgtctgcatt tttcatttgg
     1801 aagaccgact attcttggaa gtattataca gatccgcttg tctcattgat aattaccggt
     1861 ataatttttt cctctgcgct tcctctatcg tgcaaggctt ccaaaatatt gttacaagcg
     1921 acaccttcca ctttatccgg cgatcaagta gaaggtgatc ttttgaaaat accaggaata
     1981 atagctattc atgatttccg tgtttggaat ttaacagagt ctatttttat tgcatctttg
     2041 catattcaac tagatatcag cccggaacaa tttactgacc tggccaaaat agttagatca
     2101 aaacttcacc gctatggcat tcactccgct actttgcaac ctgaatttat taccagagag
     2161 gttacttcaa ccgaaagagc cggagactcc caaggtgatc atctacaaaa tgacccgctt
     2221 tcattaaggc caaagacata tggtactggc atttcaggtt ccacttgtct tgtcgacgac
     2281 gctgccaact gcaacacagc tgattgctta gaggatcatt aaaaggactt tataacgaaa
     2341 aagcgtgaag aattaaatac ttttaaaaat cgttatacgg tacatataca tacacacaca
     2401 cacatatata tagtcgtcta taatgttttt atacaatgtt tatcataaat tttgttacag
     2461 taagaagtat aggagataat ataaaaaaag aggaaaagcg aaaacaggtg ataataatga
     2521 tgatcataat aataatataa tagcaatagt cttgcaaaac aacaacttga ggaaggaaaa
     2581 tagtaataat ggtgtttagc tttttttaga ttactccagc ttctaaaaaa gagtacaata
     2641 cttagcacaa taatgctgtt atcttttttc attta
//