From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: E.chrysanthemi genes inh, prtD, prtE and prtF
Date: 5 Jan 93 06:46:26 GMT
Distribution: bionet
Checksum: 30434 11

LOCUS       ERWGENES     5435 bp ds-DNA             BCT       04-JAN-1993
DEFINITION  E.chrysanthemi genes inh, prtD, prtE and prtF
ACCESSION   X53253
KEYWORDS    protease inhibitor gene; prtD gene; prtE gene; prtF gene;
            secretion.
SOURCE      Erwinia chrysanthemi DNA.
  ORGANISM  Erwinia chrysanthemi
            Prokaryotae; Gram-negative facultatively anaerobic rods;
            Enterobacteriaceae.
REFERENCE   1  (bases 1 to 5435)
  AUTHORS   Letoffe,S., Delepelaire,P. and Wandersman,C.
  TITLE     Protease secretion by Erwinia chrysanthemi: the specific secretion
            functions are analogous to those of Escherichia coli
            alpha-haemolysin
  JOURNAL   EMBO J. 9, 1375-1382 (1990)
  STANDARD  full automatic
COMMENT     From EMBL    entry ECGENES;  dated 30-DEC-1992.
FEATURES             Location/Qualifiers
     CDS             335..697
                     /gene="inh"
                     /product="protease inhibitor"
                     /codon_start=1
                     /translation="MKQLIIATLLSALSGGCMASSLRLPSAAELSGQWVLSGAEQHCD
                     IRLNTDVLDGTTWKLAGDTACLQKLLPEAPVGWRPTPDGLTLTQADGSAVAFFSRNRD
                     RYEHKLVDGSVRTLKKKA"
     CDS             714..2441
                     /function="protease secretion"
                     /gene="prtD"
                     /note="start codon gtg"
                     /codon_start=1
                     /translation="MNASSERDRSLFGVLRQFRRSFWSVGIFSAVINVLMLAPSVYML
                     QVYDRVLASGNGITLLMLTLLMAGLCAFMGALEWVRSLLVVRLGTRIDLALNQDVFNA
                     AFARNLEAGDGRAGLALTDLTLLRQFITGNALFAFFDVPWFPLFLLVLFLLHPWLGML
                     ALGGTVVPGGVGLAEPASDQSTAGGSNQQSQQATHLADAQLRNADVIEAMGMLGNLRR
                     RWLARHYRFISLQNLASERAAAVGGASKYSRIALQSLMLGLGALLAIDGKITPGMMIA
                     GSILVGRVLSPIDQLIGVWKQWSSARIAWQRLTRLIAAYPPRPAAMALPAPEGHLSVE
                     QVSLRTAQGNTRLQNIHFSLQAGETLVILGASGSGKSSLARLLVGAQSPTQGKVRLDG
                     ADLNQVDKNTFGPTIGYLPQDVQLFKGSLAENIARFGDADPEKVVAAAKLAGVHELIL
                     SLPNGYDTELGDGGGGLSGGQRQRIGLARAMYGDPCLLILDEPNASLDSEGDQALMQA
                     IVALQKRGATVVLITHRPALTTLAQKILILHEGQQQRMGLARDVLTELQQRSAANQAR
                     MNPTAAMPQ"
     CDS             2455..3801
                     /function="protease secretion"
                     /gene="prtE"
                     /codon_start=1
                     /translation="MTGMDITTQDELNEAAMRDRASRDEERALRLGWWLVLAGFGGFL
                     LWALLAPLDKGVAVQGNVVVSGNRKVIQHMQGGIVDRIQVKDGDRVAAGQVLLTLNAV
                     DARTTSEGLGSQYDQLIAREARLLAEQRNQSSLAATPRLTQARQRPEMAAIIALQEDL
                     LRSRQQSLKLEIDGVRASIDGLETSLGALQKVMSSKQSEQATLSQQLQGLRPLAADNY
                     VPRNKMLETERLFAQVSGELAQTSGEVGRTRRDIQQQKLRIAQRQQEYDKEVNSELSD
                     VQAKLNEVISQREKADFNLANVQVRAPVAGTVVDMKIFTEGGVIAPGQVMMDIVPEDQ
                     PLLVDGRIPVEMVDKVWSGLPVELQFTAFSQSTTPRVPGTVTLLSADRLVDEKDGTPY
                     YGLRIQVSEEGKRSLHGLEIKPGMPVQGFVRTGERSFINYLFKPLMDRMHLALTEE"
     CDS             3814..5202
                     /function="protease secretion"
                     /gene="prtF"
                     /codon_start=1
                     /translation="MRRKAVLLTVVLSLSGGSAQAMGLLDAWELALRNDAQLRAAGFE
                     RDAGQEEVAIGRAGLLPSLQYTYGANYSHSKVTQRDRTLNNTTKRDYDNYVSTLTLRQ
                     PLLDYAAWARYQQGVTRKLMADQRFRDRSQDLMVRLYQSWSEALLAQEKLMLLDAQRR
                     AYQEQLALNRRLLAAGEGTQTDLRETEARYTVTEAQRIEQEDTLDAAMTDLENMMGSP
                     LQIQDLSPLALDTLPDNVTENRSLSQWRELTVRHNAKLAVQRENVDYSRYEIERNRAG
                     HLPTLDLVASTRNSLSESEYNYNQKYDTQTVGLQVRVPLYSGGAVSASMRQAAAEYQQ
                     SQAELDNQTRQTFAELRRQFNLCANGAAKIRAWQMSVAAAEEAIRATRQSVAGGERIN
                     LDVLMAEQEWYNARRELTEVKYRWLQAWLNLRYTAGTLNEQDMMQLAAWFQSAPVINK
                     TGINAATGNKTN"
BASE COUNT     1146 a   1399 c   1763 g   1127 t
ORIGIN      
        1 gaattcgttc cggatcttcg ctttgtggat aatttctcgg gaaaaggaaa cgaagtggtg
       61 ttgaattggg atagtcagtc tcatcaaacc aatatgtggt tgcatttaag cgggcacgag
      121 acggctgatt ttctggtcaa cattgtcggt gcggcattac aaccgtcgga tgttattgtt
      181 taaaaataac gggaacgctc ccgctatttt cgggtttgtt gaataacggc aggtgttttc
      241 tcttatcgga aatatgcctg ccgttatcaa catatttaac cagatggttt caattattaa
      301 acgcgttcac tgatgaccga atgatgggaa tatcatgaaa cagcttatta ttgcgacatt
      361 attgagtgca ctgagtggag gatgtatggc gagcagtctg agattgccgt ccgcggcgga
      421 actgagcggg cagtgggtat tgagcggcgc agagcagcac tgtgatatcc ggttgaacac
      481 cgatgtgctt gacggcacga cctggaagct ggctggcgac acggcgtgcc tgcaaaaatt
      541 gctgccggaa gcgccggtcg gttggcggcc gacgcccgac ggcttgacgt tgacgcaggc
      601 agacggctcg gcggtggcgt ttttcagccg caaccgcgat cgctacgaac ataagctggt
      661 tgacggcagc gttcgcaccc tgaaaaagaa agcctaaggg agagagccct gccgtgaatg
      721 cttcctctga gcgagatcgt tcgttgttcg gcgtgctgcg ccagttccgc cgcagtttct
      781 ggagcgtcgg gattttcagc gccgtcatca atgtgctgat gctggcgcct tcggtatata
      841 tgctgcaggt gtatgaccgg gtgctggcgt ccggcaacgg tataaccctg ctgatgctga
      901 cgttgctgat ggcgggactg tgcgccttta tgggcgcgct ggagtgggtt cgtagtctgt
      961 tggtggtgag gctgggcacc cgtatcgatc tggcgctcaa tcaggatgtc ttcaatgcgg
     1021 ccttcgcccg taatctggag gccggcgatg gccgcgccgg gctggcgctg accgacctga
     1081 cgctgctgcg ccagttcatc accggcaatg cgctgttcgc cttcttcgat gtgccctggt
     1141 ttccgctgtt tttgctggtg ctgtttttgc tgcacccctg gctggggatg ctggcgctgg
     1201 gcggcaccgt ggtgcctggt ggcgttggcc tggctgaacc agcatctgac caatcaaccg
     1261 ctggcggaag caatcagcaa tcgcaacagg cgacccatct ggctgacgct cagttgcgca
     1321 atgccgacgt tattgaagca atggggatgc tgggcaacct gcgtcggcgc tggctggcgc
     1381 gtcattaccg ttttatttcc ctgcagaatc tggccagcga gcgtgccgcc gcggtaggcg
     1441 gtgcgtccaa atacagccgt attgcgttgc agtcgttgat gctggggctg ggagcattgc
     1501 tggcgatcga cggcaagatc acgccgggga tgatgatcgc cggttcgatt ctggttggcc
     1561 gggtactgag cccgatcgat cagttaatcg gggtatggaa acagtggagc agcgcgcgca
     1621 ttgcctggca gcgcctgacc cggctgatcg ccgcctatcc gccgcgcccg gcggcaatgg
     1681 cgctgcctgc gccggaaggg catctcagtg tggaacaggt gtcgctgcgc acggcgcagg
     1741 gcaatacccg actgcagaac atccatttct cgttgcaggc tggcgaaacg ctggtgattc
     1801 tcggcgcgtc cggttccggt aaatcctcgc tggcgcgctt gctggtcggc gcgcagtcgc
     1861 cgacgcaagg caaagtgcgt ctggatggcg ctgatttgaa tcaggtggac aaaaacacgt
     1921 ttggtccgac catcggctat ttgccgcagg atgtgcagct gtttaagggc tcgctggcgg
     1981 aaaacatcgc ccgttttggc gatgccgatc cggaaaaagt ggtggcggcc gcaaaactgg
     2041 cgggggtaca tgaactgatt ttgtcgctac ccaatggcta tgacaccgaa ttgggcgatg
     2101 gcggcggcgg tttgtccggc ggccagcggc agcgtatcgg tctggcgcgt gccatgtacg
     2161 gcgatccttg cctgctgatt ctggatgaac cgaacgccag ccttgacagc gaaggcgatc
     2221 aggccttgat gcaggcgatt gtcgcgttgc agaaacgcgg cgccaccgtg gtgttgatta
     2281 cccatcgccc ggcgctgacg acgctggcgc aaaaaattct gatcctccat gagggccagc
     2341 agcagcgtat gggactggcg cgggatgtgc tgacggaact gcaacagcgc agcgccgcca
     2401 atcaggcccg gatgaacccc acggccgcaa tgccgcaatg acagggatct cgtgatgaca
     2461 ggcatggata taaccactca ggatgaattg aacgaagcag caatgcgcga tcgcgccagc
     2521 cgggatgaag agcgcgcgtt acggctgggc tggtggctgg tgctcgccgg ttttggcggt
     2581 tttctgctgt gggccttgct ggcgccgttg gataaagggg tggcggtgca aggcaatgtg
     2641 gtggtgtccg gtaatcgcaa ggtgatccaa cacatgcagg gcggcattgt cgaccggatt
     2701 caggtgaaag acggcgaccg cgttgccgcg ggtcaggtgt tgctgacgct gaatgcggta
     2761 gatgcccgta ccaccagcga agggctgggc tcccaatacg atcaattgat tgctcgtgaa
     2821 gcccgactgc tggcggaaca acgtaatcaa tcgtcgctgg cggcgacgcc ccggctgact
     2881 caggcgcgcc agcggccgga aatggccgcc atcattgcgt tgcaggaaga tctattgcgt
     2941 agccggcagc agtcgctaaa gctggaaata gacggagtgc gcgccagcat cgatggcctg
     3001 gagacgtcgc ttggcgcatt gcaaaaggtg atgagcagca aacagtccga gcaggcgacg
     3061 ttgagccagc agttgcaggg actgcggccg ctggcggcgg ataactatgt tccgcgtaat
     3121 aagatgctgg aaacggagcg cttgtttgcg caggtcagcg gcgaactggc ccagaccagc
     3181 ggcgaggtag ggcgaacccg gcgtgatatt cagcagcaaa aattgcgcat cgcccagcgt
     3241 cagcaggaat acgacaaaga ggtcaacagc gaattgtccg acgtacaggc caaactgaat
     3301 gaggtgatca gtcagcgcga gaaggcggat ttcaatctgg ccaacgtgca ggtccgtgca
     3361 ccggttgccg gcaccgtggt ggatatgaag atcttcaccg aaggcggggt gatcgccccc
     3421 gggcaggtca tgatggacat cgttcccgaa gaccagccgc tgctggtgga tgggcgcatt
     3481 ccggtggaaa tggtggacaa ggtgtggtcc gggctgccgg tggaactgca gttcaccgcg
     3541 tttagccaaa gcaccacgcc gcgagtgccg ggaacggtga ccctgctttc cgctgaccgg
     3601 ctggtggacg aaaaggacgg cacgccgtac tacggtttgc gcattcaggt gagcgaagaa
     3661 ggcaagcgtt cgttgcacgg gctggaaatt aaacctggta tgccggtgca ggggtttgtc
     3721 cgcaccggag agcgttcgtt tatcaattat ctgtttaaac ccctgatgga tcgcatgcat
     3781 ctggcgttga cggaagagta acggaaggaa gcaatgcgaa ggaaagcggt actactgacg
     3841 gtcgtgttat ctctgtcggg cggcagcgcc caggcgatgg ggctgctgga tgcatgggaa
     3901 ctggcgctgc gtaatgacgc acagctgcgg gcggccggtt tcgaacgaga cgccgggcag
     3961 gaagaggtgg cgatcggccg cgccgggttg ctgccgtcgc tgcaatatac ctatggcgct
     4021 aattacagtc attccaaggt gacgcaacgc gatcggacgc tgaataacac gactaaacgt
     4081 gactacgata actacgtatc gacgttgacg ctgcgtcagc cgctgctgga ctatgccgcc
     4141 tgggcgcgct accagcaggg cgtcacccgc aaactgatgg ccgatcagcg atttcgcgat
     4201 cgcagtcagg acctgatggt gcgtttgtac cagtcctgga gcgaggcatt gctggcgcag
     4261 gagaaattga tgctgctgga tgcgcagcgc cgtgcctatc aggagcagtt ggcgctgaac
     4321 cgtcgcttat tggcggcggg ggaaggcacg caaaccgacc tgcgggaaac ggaggcgcgt
     4381 tacaccgtca ctgaagccca gcgtatcgaa caggaagata cgctggatgc cgctatgacc
     4441 gatctggaaa acatgatggg ttcgccgttg caaattcagg atctgtcgcc gttggcgctg
     4501 gatacgttgc cggataatgt caccgaaaat cgctcgctgt cgcagtggcg cgaactgacg
     4561 gtgcggcaca acgcaaagct ggcggtacag cgtgaaaacg tcgattacag ccgctacgaa
     4621 attgagcgta atcgcgccgg ccatctgccg acattggatt tggtggcgtc cacccgcaac
     4681 agcttgtcgg agtctgaata caactacaac cagaaatacg acacccagac cgtcggtttg
     4741 caggtgcgcg tgccgctgta ttccggcggc gccgtttcgg cctcgatgcg tcaggcagcg
     4801 gcggaatacc agcaaagtca ggcggagctg gataaccaga cccggcagac ctttgcggaa
     4861 ttacgccgtc agttcaacct gtgcgccaat ggggccgcca aaatccgagc ctggcagatg
     4921 agcgtggccg ccgctgagga agcgatccgc gcgacgcggc agagcgtcgc cggcggcgag
     4981 cgcatcaatc tggacgtgct gatggcggaa caggagtggt ataacgcccg ccgcgagctg
     5041 acggaagtca aatatcgctg gttacaggcc tggctgaatt tgcgctatac cgccggaacg
     5101 ctgaatgagc aggatatgat gcaattggct gcctggtttc aatctgcgcc tgtaataaat
     5161 aaaaccggaa taaacgcggc caccggtaat aaaacgaatt agcggtaacc tttggcgtta
     5221 gttggttgtg gtgtctatga aaactgatgg ttttctctac aaaaattgat tgcagtttcc
     5281 acgaaatttc tttcgcagcc cgctgcgaga gatgggtatg tcattaaatt agccgtgatg
     5341 ataattgccg gcgatttaat ggcgaaacaa ggcattctga cagtaatgcg tcagacttta
     5401 tggaagaagt gatatgcaac aaaacgaaaa agctt
//