From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Mycobacterium organophosphate acid anhydrase (opaB) gene, complete
Date: 9 Apr 92 10:04:13 GMT
Distribution: bionet
Checksum: 03748     4

LOCUS       MSGOPAB      1600 bp ds-DNA             BCT       09-APR-1992
DEFINITION  Mycobacterium organophosphate acid anhydrase (opaB) gene, complete
            cds.
ACCESSION   M91040
KEYWORDS    opaB gene; organophosphate acid anhydrase.
SOURCE      Mycobacterium sp. (strain B-1) DNA.
  ORGANISM  Mycobacterium sp.
            Prokaryota; Bacteria; Firmicutes; Mycobacteria; Mycobacteriaceae.
REFERENCE   1  (bases 1 to 1600)
  AUTHORS   Mulbry,W.W.Dr..
  TITLE     The organophosphate acid anhydrase gene opaB from the gram-positive
            bacterial strain B-1: Cloning, nucleotide sequence, and expression
            in E. Coli
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     -35_signal      15..19
                     /gene="opaB"
     -10_signal      38..43
                     /standard_name="PRIBNOW BOX"
                     /gene="opaB"
     RBS             170..174
                     /gene="opaB"
     CDS             181..1410
                     /product="organophosphate acid anhydrase"
                     /gene="opaB"
                     /evidence=EXPERIMENTAL
                     /codon_start=1
                     /translation="MSAEFVIRNAKVFDGERVYERLDVAVREGRIAQVGTDLATPADG
                     DELDAAGQALLPGLIDGHAHAQPPALAHALRFGVTTVLDMFSMPDWMDAQRLEAAERN
                     DLADVRSASVGATVLGGLPSMLIGSFFPTPFPVLATVDDAPAFVEARVNEGADYIKLL
                     IDDGTALGHTGPSLTEEMAKAVVEAAHVHGKMAVAHATSLDGVAQAIRAGVDGLVHIF
                     FDRAPTDEIVRLAKEADVFVTPTLSTVGSLASDIDGSHLAHDPRAEELIPAEWKQNLC
                     QCWHLGSPGSVDHALAATKALHDAGVEILAGTDATCVGVAGTAHGISMHGELELLVRA
                     GLTPIEALRAATSATARRFNLGDRGRIAVGLQADLLLVDGDPTTQITDTLSISRVWRR
                     GVQLDRTPAQLTEAARV"
                     /citation=[1]
BASE COUNT      281 a    554 c    529 g    236 t
ORIGIN
        1 tgagagtcaa gagattgacg actgcgtcga agcgtcgtag actgtgacag cggccacacc
       61 ggacgttcgc ctcggtcggg ccctctgtgg aagccgggca gaaccccgcc gacttcagga
      121 cgggcgtccg cgtccaggag atccgcgagc cgacccaccg tcacgccgag gaggcatgag
      181 atgtcagcag agttcgtcat ccggaacgcg aaggtcttcg acggcgagcg tgtgtacgag
      241 cgactcgacg tggccgttcg cgaaggccga atcgcgcaag tcggcaccga cctcgcgact
      301 cctgcggacg gcgacgagct cgacgccgcg ggccaggcgc tacttccggg cctcatcgat
      361 gggcacgccc atgcccagcc gccggcgctg gcgcacgccc tacggttcgg cgtcaccacc
      421 gtgttggaca tgttctcgat gccggactgg atggatgctc aacgccttga ggccgctgag
      481 cgcaacgacc tcgccgacgt caggtcggca tccgtgggcg cgaccgtgct gggtgggctt
      541 ccatcgatgc tgatcggcag cttcttcccc acaccgttcc ccgttctggc aaccgtcgat
      601 gacgcaccag cgtttgtgga ggcacgcgtc aacgagggcg ccgactacat caagctgctc
      661 atcgacgacg gaacggctct cggccacacc gggcccagcc tgaccgagga gatggctaag
      721 gctgtggtcg aagcggccca tgtgcacggg aagatggcgg tcgcacacgc cacgagtctg
      781 gacggggtgg cccaggccat tcgagcaggg gtcgacggcc tcgtgcacat cttcttcgac
      841 cgcgccccga cggacgagat cgtgcgcctc gccaaggaag ccgacgtctt cgtcacgccg
      901 accctgagca ccgtgggctc cctggcaagc gacatcgacg gttcccacct cgcgcacgat
      961 ccccgcgccg aggagctgat cccagccgaa tggaagcaga acctctgcca gtgctggcac
     1021 ctcggttcac cgggcagcgt cgaccatgcg ctcgcagcaa caaaggcgct ccacgacgca
     1081 ggcgtggaga ttctcgccgg cacggacgct acctgcgtgg gcgtcgcagg gaccgctcac
     1141 ggcatcagca tgcacggtga gcttgagttg ctggtacgcg ccggcctcac cccgatcgag
     1201 gcgctgcggg cagcaacctc cgccaccgcg cgtcgcttca acctgggcga tcgaggccgc
     1261 atcgccgtgg gtctgcaagc agaccttctg ctcgtcgacg gcgacccgac gacgcagatc
     1321 accgacaccc tctcgatcag ccgggtctgg cgacgcgggg tgcagctcga ccggacgccc
     1381 gcgcagctga ccgaggcggc ccgggtctag acgacgggcc ccgcggaaag gcccatcatt
     1441 caccagcgga acgatgatgc cgcggtcggc gacgccctgg tctagagctc ctccggccag
     1501 aggccgtctt cggatcgacc ggaggcggcc cctgttccat gtgcgagccg aaatgggtca
     1561 gctgcctgct gaggtggcga cgacaagaca cgcgagccac
//