From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: B.stearothermophilus genes for glyceraldehyde-3-phosphate
Date: 3 Jan 93 22:03:33 GMT
Distribution: bionet
Checksum: 30012 4

LOCUS       BAC3PHKIN    1431 bp ds-DNA             BCT       21-DEC-1992
DEFINITION  B.stearothermophilus genes for glyceraldehyde-3-phosphate
            dehydrogenase and 3-phosphoglycerate kinase
ACCESSION   X58059
KEYWORDS    3-phosphoglycerate kinase;
            glyceraldehyde-3-phosphate dehydrogenase; glycolytic enzyme;
            intercistronic region; thermostable enzyme.
SOURCE      Bacillus stearothermophilus DNA.
  ORGANISM  Bacillus stearothermophilus
            Prokaryotae; Firmicutes; Endospore-forming rods and cocci;
            Bacillaceae.
REFERENCE   1  (bases 1 to 1431)
  AUTHORS   Davies,G.J., Littlechild,J.A., Watson,H.C. and Hall,L.
  TITLE     Sequence and expression of the gene encoding 3-phosphoglycerate
            kinase from Bacillus stearothermophilus
  JOURNAL   Gene 109, 39-45 (1992)
  STANDARD  full automatic
COMMENT     *source: strain=NCA 1503; *source: clone=pBstPGK-1;
               From EMBL    entry BS3PHKIN;  dated 17-NOV-1992.
FEATURES             Location/Qualifiers
     CDS             1..66
                     /partial
                     /partial
                     /product="glyceraldehyde-3-phosphatedehydrogenase"
                     /codon_start=1
                     /translation="NETGYSHRVVDLAAYIASKGL"
     CDS             188..1372
                     /EC_number="2.7.2.3"
                     /product="3-phosphoglycerate kinase"
                     /codon_start=1
                     /translation="MNKKTIRDVDVRGKRVFCRVDFNVPMEQGAITDDTRIRAALPTI
                     RYLIEHGAKVILASHLGRPKGKVVEELRLDAVAKRLGELLERPVAKTNEAVGDEVKAA
                     VDRLNEGDVLLLENVRFYPGEEKNDPELAKAFAELADLYVNDAFGAAHRAHASTEGIA
                     HYLPAVAGFLMEKELEVLGKALSNPDRPFTAIIGGAKVKDKIGVIDNLLEKVDNLIIG
                     GGLAYTFVKALGHDVGKSLLEEDKIELAKSFMEKAKEKGVRFYMPVDVVVADRFANDA
                     NTKVVPIDAIPADWSALDIGPKTRELYRDVIRESKLVVWNGPMGVFEMDAFAHGTKAI
                     AEALAEALDTYSVIGGGDSAAAVEKFGLADKMDHISTGGGASLEFMEGKQLPGVVALE
                     DK"
BASE COUNT      335 a    368 c    442 g    286 t
ORIGIN      
        1 aacgaaacgg gctattcgca ccgcgtcgtc gacttagctg cctacatcgc ctcgaaaggg
       61 ctgtaaaacc agcttggtgc tgagttttcc gcctgttcaa gtctataata ggaaaatgga
      121 ggggagcggg gaaatgatcc ccactccttt tccttgtcca aactgtagcc aaaagggggc
      181 acgaacgatg aacaagaaga cgatccgcga cgttgatgtg aggggaaagc gcgtcttttg
      241 ccgcgtcgat ttcaacgttc cgatggagca aggcgctatc accgatgaca cgcgcattcg
      301 cgccgcactc ccgacgatcc gctatttgat cgagcacggg gcgaaagtca ttttggcgag
      361 ccacctcggc cgcccgaaag gaaaagtggt cgaagaattg cgtttggatg ccgttgcgaa
      421 gcggctcggc gagctgcttg aacggccggt tgccaaaacg aatgaagcgg tcggcgatga
      481 ggtgaaagcg gcggtcgacc gtttgaacga aggcgatgtg ctcttgcttg agaacgtccg
      541 tttttaccct ggcgaagaga aaaatgatcc cgagctcgcc aaagcgtttg cggagcttgc
      601 ggatctatat gtcaacgatg cgttcggcgc cgcccatcgc gctcatgcgt cgacggaagg
      661 catcgcccat tacttgccgg cggtggccgg atttttgatg gaaaaagaac ttgaagtgct
      721 tggcaaggcg ctttcgaatc cggaccgccc gtttacagcg atcatcggcg gggcgaaagt
      781 gaaagacaaa atcggcgtca tcgacaattt gcttgaaaaa gtcgacaact tgatcatcgg
      841 cggcggactg gcgtatacgt tcgtcaaagc gctcggccat gacgtcggca agtcgctgct
      901 tgaggaggac aaaatcgaac tcgccaaatc gtttatggaa aaggcgaaag aaaaaggcgt
      961 ccgtttttat atgccggtgg acgtggtcgt cgccgaccgg tttgcgaacg acgccaacac
     1021 gaaagtcgtg ccgattgacg cgattccagc cgattggtcg gcgcttgaca tcggcccgaa
     1081 aacgcgcgaa ttgtaccgcg atgtcattcg cgagtcgaag ctcgttgtct ggaacggccc
     1141 gatgggcgtc tttgaaatgg acgcgttcgc ccacgggaca aaagcgatcg ccgaagcact
     1201 ggcggaagcg ctcgacacct attcggtcat cggcggcggg gattcggcgg cggcggttga
     1261 gaaattcggc ttggccgaca aaatggatca tatctccacc ggcggcggcg cttcgctcga
     1321 gtttatggaa ggaaaacagc tgccgggtgt cgtcgcactc gaagacaaat gacccacggc
     1381 gccggatttg gcgcatggcg gctgactccg cccattgaaa ggaagggtga a
//