From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Klebsiella pneumoniae sodium-dependent citrate carrier protein
Date: 21 Jul 92 10:06:42 GMT
Distribution: bionet
Checksum: 34654     5

LOCUS       KPNCITS      2083 bp ds-DNA             BCT       21-JUL-1992
DEFINITION  Klebsiella pneumoniae sodium-dependent citrate carrier protein
            (citS) and oxaloacetate decarboxylase gamma-subunit genes, complete
            cds.
ACCESSION   M83146
KEYWORDS    citS gene; citrate carrier protein;
            oxaloacetate decarboxylase gamma-subunit.
SOURCE      Klebsiella pneumoniae (library: ATCC 13882) DNA.
  ORGANISM  Klebsiella pneumoniae
            Prokaryotae; Gram-negative facultatively anaerobic rods;
            Enterobacteriaceae.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   van der Rest,M.E., Siewe,R.M., Abee,T., Schwarz,E., Oesterhelt,D.
            and Konings,W.N.
  TITLE     Nucleotide sequence functional properties of a sodium-dependent
            citrate transport system from Klebsiella pneumoniae
  JOURNAL   J. Biol. Chem. 267, 8971-8986 (1992)
  STANDARD  full staff_entry
FEATURES             Location/Qualifiers
     -35_signal      159..164
                     /gene="citS"
     -10_signal      182..187
                     /gene="citS"
     RBS             309..312
                     /gene="citS"
     CDS             321..1661
                     /product="citrate carrier protein"
                     /gene="citS"
                     /evidence=EXPERIMENTAL
                     /codon_start=1
                     /translation="MTNMSQPPATEKKGVSDLLGFKIFGMPLPLYAFALITLLLSHFY
                     NALPTDIVGGFAIMFIIGAIFGEIGKRLPIFNKYIGGAPVMIFLVAAYFVYAGIFTQK
                     EIDAISNVMDKSNFLNLFIAVLITGAILSVNRRLLLKSLLGYIPTILMGIVGASIFGI
                     AIGLVFGIPVDRIMMLYVLPIMGGGNGAGAVPLSEIYHSVTGRSREEYYSTAIAILTI
                     ANIFAIVFAAVLDIIGKKHTWLSGEGELVRKASFKVEEDEKTGQITHRETAVGLVLST
                     TCFLLAYVVAKKILPSIGGVAIHYFAWMVLIVAALNASGLCSPEIKAGAKRLSDFFSK
                     QLLWVLMVGVGVCYTDLQEIINAITFANVVIAAIIVIGAVLGAAIGGWLMGFFPIESA
                     ITAGLCMANRGGSGDLEVLSACNRMNLISYAQISSRLGGGIVLVIASIVFGMMI"
     CDS             1817..2068
                     /product="oxaloacetate decarboxylase gamma-subunit"
                     /gene="oxaloacetate decarboxylase gamma-subunit"
                     /codon_start=1
                     /translation="MTDNAVLLGEGFTLMCLGMGFVLVFLLLLIFAIRGMSLAVNRLF
                     PEPPAAPKPAPAAVAPADDFARLKPAIVAAIHHHRRLHP"
BASE COUNT      441 a    570 c    508 g    564 t
ORIGIN
        1 gatcgatcgc ctctcgccgc ggtttatctt ttaatagctg aacgcgtttt aatatcagtg
       61 tcatttttta gcgcttcgtc atttcaaaac gaactgtatt tcgccagcgt tcggctatgg
      121 attcttgtat ttaagtcaca gtttttctcg tggcttaatt ggctttatgg tttttatgtt
      181 gttaattaat tctgaatcat aaaaacaatt aaattaataa aaaacacgag tattcagtga
      241 agataatcac accttacccc tggatctatt tattactatg cgccggtctt actactccat
      301 caataaacag gataacgttt atgactaaca tgagtcagcc tccggcgacg gagaaaaagg
      361 gcgttagcga tctgctggga tttaaaatct tcggcatgcc gctccccctt tacgcatttg
      421 cgttaattac actattactt tcacattttt ataacgcttt acccaccgat atcgtcggcg
      481 gctttgccat catgtttatt atcggcgcta tattcgggga aattggcaag cgcctgccaa
      541 tattcaacaa atatatcggt ggcgctccgg tgatgatttt cctggtggcg gcttatttcg
      601 tctacgccgg tatttttacg cagaaagaaa tcgatgctat cagcaacgtt atggataaga
      661 gtaacttcct taacttattc atcgcggtat taattaccgg agcaattctg tcggtaaacc
      721 gccgcctgct gctgaaatca ctgctcggct atattccaac tatcctgatg ggcattgtcg
      781 gcgcgtctat ttttggcatc gctatcggcc tggtgttcgg tattccagtg gatcgcatca
      841 tgatgctgta cgtcctgccg atcatgggcg gcggcaacgg cgcgggcgcg gtccccctgt
      901 cggagattta ccactccgtc accggtcgtt ctcgcgaaga gtattactca accgctatcg
      961 ctattctgac catcgctaac atcttcgcca tcgtctttgc cgccgtcctc gatatcatcg
     1021 gcaaaaaaca cacctggctg agcggcgaag gggaactggt gcgtaaagcc tcgttcaaag
     1081 tggaagaaga tgagaaaacg ggacaaatca cccatcgcga aaccgccgtc ggtctggtgc
     1141 tgtccactac ctgcttcctg ctggcctacg tcgtggcgaa gaaaattctg cccagcattg
     1201 gcggtgtggc tattcactat ttcgcgtgga tggtgctgat cgtcgccgcg ctgaacgcct
     1261 ccgggctgtg ctcaccggaa atcaaagccg gtgctaaacg cttgtctgat ttcttctcca
     1321 aacagctgct gtgggtgctg atggtcgggg tcggcgtctg ctacaccgac ctacaggaaa
     1381 ttatcaacgc catcaccttc gctaacgttg ttatcgccgc cattatcgtt atcggtgcgg
     1441 tgctcggcgc ggcgatcggt ggctggctga tgggcttctt cccaatagaa tcggccatca
     1501 ctgcaggcct gtgtatggcc aaccgcggcg gctccggcga cctggaagtt ctctctgcct
     1561 gtaaccgtat gaaccttatc tcttatgcgc aaatctcctc ccgtttgggc ggcggtattg
     1621 tgctggtcat cgccagcatc gtgttcggca tgatgattta acccatacgc acgatggcac
     1681 gttgaactga caggtgaaga acgaatcagg gtcccatgcc gggagcggca gagggaaatc
     1741 caccctgctc cttagccgga aacgccagaa accttgtcag acagcgcgtc atcgtgcccc
     1801 taattgagat gtataaatga ctgacaatgc cgtcctgtta ggcgaaggct tcaccctgat
     1861 gtgtctcggc atgggctttg tgctggtgtt cctgctgctg ctcattttcg ccatccgggg
     1921 aatgtccctc gccgtcaacc gcctgtttcc tgagccgcct gccgccccca aaccggcgcc
     1981 ggccgccgtc gcgcccgccg acgatttcgc ccgcctgaag ccggcgattg tcgccgccat
     2041 tcaccaccat cgccgtcttc acccttaatt cacggaggaa ttc
//