From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: D.pseudoobscura Adh gene for alcohol dehydrogenase
Date: 28 Dec 92 21:27:17 GMT
Distribution: bionet
Checksum: 30998 8

LOCUS       DROADHGN43   3477 bp ds-DNA             INV       01-DEC-1992
DEFINITION  D.pseudoobscura Adh gene for alcohol dehydrogenase
ACCESSION   X62223
KEYWORDS    ADH gene; alcohol dehydrogenase.
SOURCE      Drosophila pseudoobscura DNA.
  ORGANISM  Drosophila pseudoobscura
            Eukaryota; Animalia; Metazoa; Arthropoda; Uniramia; Insecta;
            Pterygota; Neoptera; Holometabola; Diptera; Brachycera;
            Cyclorrhapha; Schizophora; Drosophiloidea; Drosophilidae.
REFERENCE   1  (bases 1 to 3477)
  AUTHORS   Schaeffer,S.W. and Miller,E.L.
  TITLE     Estimates of gene flow in Drosophila pseudoobscura determined from
            nucleotide sequence analysis of the alcohol dehydrogenase region
  JOURNAL   Genetics 132, 471-480 (1992)
  STANDARD  full automatic
COMMENT     *source: strain=MV36; *source: isolate=Mesa Verde, Colorado;
            *source: chromosome=IV section 88;
               From EMBL    entry DPADHGN43;  dated 16-OCT-1992.
FEATURES             Location/Qualifiers
     mRNA            join(40..119,916..1021,1086..1490,1551..1966)
                     /note="adult mRNA (Adh)"
     mRNA            join(883..1021,1086..1490,1551..1966)
                     /note="larval mRNA (Adh)"
     promoter        8..14
                     /note="adult promoter (Adh)"
     prim_transcript 40..1966
                     /note="adult primary transcript (Adh)"
     exon            40..119
                     /note="adult exon 1a"
     intron          120..915
                     /note="adult intron"
     promoter        847..854
                     /note="larval promoter (Adh)"
     prim_transcript 883..1966
                     /note="larval primary transcript (Adh)"
     exon            883..1021
                     /note="larval exon 1"
     exon            916..1021
                     /note="adult exon 1b"
     intron          1022..1085
                     /number=1
     exon            1086..1490
                     /number=2
     intron          1491..1550
                     /number=2
     exon            1551..1966
                     /number=3
     polyA_signal    1943..1948
     polyA_site      1966
     CDS             join(929..1021,1086..1490,1551..1817)
                     /EC_number="1.1.1.1"
                     /gene="Adh"
                     /product="alcoholdehydrogenase"
                     /codon_start=1
                     /translation="MSLTNKNVVFVAGLGGIGLDTSRELVKRNLKNLVILDRIDNPAA
                     IAELKAINPKVTITFYPYDVTVPVAETTKLLKTIFAQVKTIDVLINGAGILDDHQIER
                     TIAVNYTGLVNTTTAILDFWDKRKGGPGGIICNIGSVTGFNAIYQVPVYSGSKAAVVN
                     FTSSLAKLAPITGVTAYTVNPGITKTTLVHKFNSWLDVEPRVAEKLLEHPTQTSQQCA
                     ENFVKAIELNKNGAIWKLDLGTLEPITWTQHWDSGI"
BASE COUNT      972 a    788 c    797 g    920 t
ORIGIN      
        1 cgtagtgtat ttaaggcact gcacatgtcg aggaccacga ttattgtcta cagtgcagtc
       61 agtagttgtc aggtgcagca ggcggaccag catttcttta aatctactca atcgatcaag
      121 taagtaactg taatccgagt acagtcggcc catcccactc ggttctgcag taactcaaga
      181 aatttcttga tagcgcagtt ccagttgtag aacgtcgttt aaaaaaacat ctcgttggag
      241 cttgggttga aaaacgttcg agttgggcgt agaaaatttc aaatataaaa tatcaaaaaa
      301 gagaaaatat cttcccaaag cagctgtcca tttttttgtt aaagtgatag atcaaaaatt
      361 cgtatgacgt atttgccaag tgctctttat gcaaataatt tcctcaattc taattctaca
      421 agaagcactt ttggctcttg tccagaaaaa acaatttgtt tacactttgc ggttgataac
      481 agaagcggac cgacggctaa gtaacataac agtcgcctgt taagtaatgc cctcactcgc
      541 tgttagatgg ccgcgcgtgt cgactccgat agaccagcag acacgcagta gttttcctca
      601 agaacctgaa ctctaaacat agacataatt tacgatacct tgagactcaa ataaaaatac
      661 aagtatgagt ggtattctat tcctgattag actcgcacac acacacgtac gagtattaac
      721 ataaatttgc cggcaaataa ccaaaacaaa acgaacgcaa acgatccgaa cacgtaatgc
      781 gagagataag aaacagaaaa gctcgacgtg aaagaagctt ctggcggaag ctcttgccga
      841 actccctata aatacgagac agaaaaccag aataaatata ataacaaact gttgaattgt
      901 cccatcgatt cccagatcaa aagacagaat gtctctcacc aacaagaacg tcgttttcgt
      961 ggccggtctg ggcggcattg gcctggacac cagccgggag ttggtcaagc gtaatctgaa
     1021 ggtaagagag aacgaattcc atggaatcta tggaaatcct aaatttaaaa atttattttt
     1081 tttagaacct ggtcatcctg gatcgcattg acaatccggc tgccattgcc gaactgaagg
     1141 caatcaatcc caaggtgacc atcaccttct atccctacga tgtgactgtg cccgtcgctg
     1201 agaccaccaa gctcctgaag accatctttg cccaggtgaa gaccatcgat gtcctgatca
     1261 acggtgctgg aatcctggac gatcatcaga tcgagcgcac cattgccgtt aactacacgg
     1321 gcctggtcaa caccaccaca gccattctgg acttctggga caagcgcaag ggcggcccag
     1381 gcggcatcat ttgcaacatt ggctccgtca ccggtttcaa tgccatctac caggtgcccg
     1441 tttactccgg ctccaaggcg gcggtggtta actttacctc ctccctggcg gtaagtaatc
     1501 caaccttttt cctactcccc aaaactaaat atttctttga aatcccatag aaactggctc
     1561 ccattactgg tgtcactgct tacactgtca atcctggcat cactaagacc actctggtcc
     1621 acaaattcaa ctcgtggctg gatgtggagc cccgtgtggc agagaagctg ctcgagcatc
     1681 ccacccagac ctctcagcag tgcgccgaga actttgtgaa ggccattgag ctgaacaaga
     1741 acggtgccat ctggaagttg gatctgggca ccttggagcc catcacatgg acccagcact
     1801 gggactcggg catctaaacc atcccagaga ctctatggga aatggcgttt tagcttttag
     1861 tttctttttt tccactcaat tgttacgtat atacatacac atatggcaat aaggctgatt
     1921 tgaaccccgt tttgaatatg ataatacaaa attatatttg agaaatttca acaaaatcga
     1981 taagagctat agctctcttt tttttaatta aaaaaaacta gataaaaaga gcaatggtca
     2041 gtggtggtca gtggtcgttg tggcctctat cgatttccac acaaaaactt tactgtttag
     2101 taatagaaaa gaacttgaga aaggcagcca aaagaatgta cgatctgacg ggtaagcatg
     2161 tctgctatgt ggctgactgc ggtggcattg cactggagac cagcaaggtt ctcatgacca
     2221 agaatatagc ggtgagtggt agagtggtgt agagagtgga gagtggttct ggttcttaaa
     2281 caagtctcta gtctttagtc tcttggctgc tgctggtagc tgtcgaaact aattaagtta
     2341 atgacttttt catgttttgt ttttgtccgc tgacttttgc tgttcaaagc tacaattaag
     2401 tttatgtttt gagtttgatc tacttttgct taagggaagt actttcgatt acggattgaa
     2461 ttgctggcat ataaaaatag ataccaagga atgtgcatca tatcatattg attctatctt
     2521 gattctttgc ccagaaactg gccatccttc agagcgtgga aaaccccccg gccatcgctc
     2581 agttacaatc gattaagcac agcacacaga tcttcttctg gaccttcgat gtgaccatgg
     2641 cccgcgagga gatgaagaag tacttcgatg aggtcatggt ccagatggac tacatcgatg
     2701 tgctgatcaa tggggccacc ctgtgcgatg agcggaacat tgatgccacc atcaatacga
     2761 atctgaccgg catgatgaac accgtggcca cggtgctgcc ctatatggac cgtaagatgg
     2821 gcggttcggg tggactgatt gtgaatgtca cctccgtcat aggactggat ccatcgccag
     2881 tcttttgtgc gtacagtgcc tcaaagtttg gtgtgattgg gttcacccga agtctagcgg
     2941 tgagtgggcg agatcttaac ttgaaaatgc tctctaatgt cttccattct ttcgcaggat
     3001 cccctgtatt acacccaaaa tggtgtggct gtcatggccg tctgctgtgg tcccaccaaa
     3061 gtgtttgtcg atcgcgaact gactgccttc ctggcatacg gccagacctt tgccgatcgc
     3121 ctgcgtcgtg ccccctgcca atcgacggcc gtctgcgggc agaatatagt aaatgccatc
     3181 gagagatcgg agaacggaca gatctggatt gccgacaagg gcggcctgga gtcggtggcc
     3241 ctccactggt attggcatat ggccgatcag tttgtgaact atatgcagag cactgacgat
     3301 gaggatcagg agtttttttt gggccagcga tgaggcatgc cttgatccac ggaataaaac
     3361 atcaaatgtg tcacaaaaag taggcaactt gtttgtgggt ctggaaatag atttgaaata
     3421 ttttgaggtc atatgaataa tgacataaat gagggtgcct gaaagtatgc caagttt
//