From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: pUC18 cloning vector.
Date: 8 Jan 93 05:51:52 GMT
Distribution: bionet
Checksum: 02484 7

LOCUS       SYNPUC18V    2686 bp ds-DNA   Circular  SYN       07-JAN-1993
DEFINITION  pUC18 cloning vector.
ACCESSION   L08752
KEYWORDS    .
SOURCE      Artificial gene DNA; Bacteriophage M13mp18 DNA; Escherichia coli
            DNA; Artificial gene DNA; Escherichia coli DNA; Cloning vector DNA;
            Cloning vector DNA; and Cloning vector DNA.
  ORGANISM  Artificial gene
            Artificial sequences; Genes.
REFERENCE   1  (sites)
  AUTHORS   Norrander,J.M., Kempe,T. and Messing,J.
  JOURNAL   Gene 26, 101-106 (1983)
  STANDARD  full automatic
REFERENCE   2  (bases 1 to 2686)
  AUTHORS   Gilbert,W.
  TITLE     Obtained from VecBase 3.0
  JOURNAL   Unpublished (1991)
  STANDARD  full automatic
COMMENT     VecBase accession number VB0025 COLLECTION  ATCC 37253
               COMMENT
              Assembled from pUC19 and M13mp18 by F. Pfeiffer, MPI, Martinsried

              Revised 16-DEC-1986 by F. Pfeiffer:
              2308/9 'AT' to 'TA' to match revised sequence of PBR322
              The strand shown contains the beta-galactosidase mRNA sequence
              including the multiple cloning site of M13mp18.

               CROSSREFERENCE
              #complement
                VecBase(3):pUC18c
              #parent
                VecBase(3):pUC12, VecBase(3):M13mp18, VecSource(3):bGal18
              #brother
                VecBase(3):pUC19
              #offspring
                VecBase(3):pGEM3, VecBase(3):pTZ18R,
                VecBase(3):pIBI24, VecBase(3):pIBI30, VecBase(3):pSP18,
                VecBase(3):pT7T318, VecBase(3):pT7T3A18 PARENT
              Features of pUC18 (2686 bp)
                residue     source
                 1- 447  6002-6448      M13mp18
                 1- 229  1069-1297      Lac-Operon
               230- 286     1-  57      polylinker of M13mp18
               289- 447  1303-1461      Lac-Operon
               448- 547  2351-2252  (c) pBR322
               548- 684  2210-2074  (c) pBR322
               685-2686  4355-2354  (c) pBR322
              Conflict (cfl) and Mutations (mut):
                   pUC18  source
              mut 1128 T  C 3912 (c) pBR322
              mut 1429 A  G 3611 (c) pBR322
               POLYLINKER
            EcoRI-SacI-KpnI-SmaI-BamHI-XbaI-SalI-PstI-SphI-HindIII SELECTION
              #resistance  Ap
              #indicator beta-galactosidase.
FEATURES             Location/Qualifiers
     CDS             952..1740
                     /product="beta-lactamase"
                     /codon_start=3
                     /translation="HPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMM
                     STFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAI
                     TMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVA
                     MATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSR
                     GIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKH"
BASE COUNT      659 a    684 c    677 g    666 t
ORIGIN      
        1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
       61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
      121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
      181 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg aattcgagct
      241 cggtacccgg ggatcctcta gagtcgacct gcaggcatgc aagcttggca ctggccgtcg
      301 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
      361 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
      421 agttgcgcag cctgaatggc gaatggcgcc tgatgcggta ttttctcctt acgcatctgt
      481 gcggtatttc acaccgcata tggtgcactc tcagtacaat ctgctctgat gccgcatagt
      541 taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc
      601 cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt
      661 caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg
      721 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc
      781 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
      841 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
      901 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
      961 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     1021 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     1081 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     1141 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     1201 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     1261 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     1321 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     1381 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     1441 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     1501 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     1561 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     1621 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     1681 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     1741 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     1801 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     1861 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     1921 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     1981 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     2041 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga
     2101 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     2161 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     2221 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     2281 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     2341 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     2401 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     2461 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     2521 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     2581 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     2641 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaaga
//