From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Porphyromonas (Bacteroides) gingivalis NAD-dependent glutamate
Date: 28 Dec 92 21:44:29 GMT
Distribution: bionet
Checksum: 26322 5

LOCUS       POYBACCSA    1953 bp ds-DNA             BCT       02-DEC-1992
DEFINITION  Porphyromonas (Bacteroides) gingivalis NAD-dependent glutamate
            dehydrogenase (gdh) gene, complete cds.
ACCESSION   L07290
KEYWORDS    NAD-dependent glutamate dehydrogenaseNAD*glu*deh;
            cell surface antigen; recombinant antigen.
SOURCE      Porphyromonas gingivalis DNA.
  ORGANISM  Porphyromonas gingivalis
            Prokaryotae; Gracilicutes; Scotobacteria; Anaerobic gram-negative
            straight, curved and helical rods; Bacteroidaceae.
REFERENCE   1  (bases 1 to 1953)
  AUTHORS   McBride,B.C., Joe,A. and Singh,U.
  TITLE     Cloning of Bacteroides gingivalis surface antigens involved in
            adherence
  JOURNAL   Arch. Oral Biol. 55, 59-68 (1990)
  STANDARD  full automatic
REFERENCE   2  (bases 1 to 1953)
  AUTHORS   Joe,A., Yamamoto,A. and McBride,B.C.
  TITLE     Characterization of recombinant and native forms of a cell surface
            antigen of Porphorymonas (Bacteroides) gingivalis
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     -35_signal      160..165
                     /gene="gdh"
     -10_signal      179..184
                     /gene="gdh"
     RBS             215..219
                     /gene="gdh"
     CDS             226..1563
                     /number=3
                     /standard_name="PgAg1"
                     /gene="gdh"
                     /note="putative"
                     /product="NAD-dependent glutamate dehydrogenase"
                     /codon_start=1
                     /translation="MKTQEIMTMLEAKHPGESEFLQAVKEVLLSVEEVYNQHPEFEKN
                     GIIERIVEPDRVFTFRVPWVDDQGKVQVNIGYRVQFNNAIGPYKGGIRFHPSVNLSIL
                     KFLGFEQMFKNALTTLPMGGGKGGADFSPKGKSEAEIMRFCQSFMTELWRNIGPDTDI
                     PAGDIGVGGREVGYMFGMYKKLAREHTGTLTGKGFEFGGSRLRPESTGFGAVYFVQNM
                     CKQNGVDYKGKTLAISGFGNVAWGVAQKATELGIKVVTIYGPDGYVYDPDGINTPEKF
                     RCMLDLRDSGNDVVSDYVKRFPNAQFFPGKKPWEQKVDFAMPCATQNEMNLEDAKTLH
                     KNGVTLVAETSNMGCTAEASEYYVANKMLFAPGKAVNAGGVSCSGLEMTQNAMHLVWT
                     NEEVDKWLHQIMQDIHEQCVTYGKDGNYIDYVKGANIAGFMKVAKAMVAQGVC"
BASE COUNT      526 a    456 c    472 g    499 t
ORIGIN      
        1 aagctcgatg catgctgcct ttgaatcaag tcaatatact taatcttttg gcgacgtatt
       61 cggatactga tttatgtact gaaaaagctg atattcggaa gggttagttt gtgatttgga
      121 ctacaaggat cttactaaac tttatttttg agaaaaggct tgaaagttcc aaacacatta
      181 gctacttttg ccggagtaat ctcttactat tatttagagt tctctatgaa gacccaagaa
      241 attatgacaa tgctggaggc taagcacccc ggcgaaagcg aattcctcca agcagtgaag
      301 gaagttcttc tctctgtaga agaagtgtac aaccaacatc ccgagttcga aaagaacggt
      361 atcatcgagc gtatcgtaga gccggatcgt gtattcacat tccgtgtacc ctgggtagat
      421 gaccaaggta aggtacaggt aaacatcggc taccgcgttc agttcaacaa tgccatcggt
      481 ccgtacaagg gcggtatccg tttccatcct tcagtgaacc tctctatcct gaagttcctc
      541 ggtttcgaac agatgttcaa gaatgcactc actactctcc ccatgggtgg tggtaaaggt
      601 ggtgccgact tctctcccaa gggtaagagc gaagccgaaa tcatgcgttt ctgccagagc
      661 ttcatgaccg aattgtggcg caacatcggc cctgacaccg acattcctgc cggtgacatc
      721 ggcgtaggcg gtcgcgaagt aggttatatg ttcggtatgt acaagaagct cgctcgcgag
      781 cacacaggta cgcttaccgg caagggattc gagttcggcg gttctcgtct gcgtcccgaa
      841 tctaccggtt tcggtgctgt ttacttcgta cagaacatgt gtaagcaaaa cggtgtagac
      901 tacaagggca aaactcttgc tatctccgga ttcggtaacg ttgcttgggg tgtggctcag
      961 aaagctaccg agttgggcat taaggttgtt acgatctacg gtcctgacgg ctatgtttac
     1021 gaccccgacg gtatcaacac accggagaaa ttccgatgca tgcttgacct ccgtgacagc
     1081 ggtaacgacg tagtatcaga ctatgtgaag agattcccca atgctcagtt cttccccggc
     1141 aagaagcctt gggagcaaaa ggtagacttc gctatgcctt gcgctacgca gaacgagatg
     1201 aacctcgaag atgccaagac gttgcacaag aatggtgtta ctcttgtagc tgaaacttct
     1261 aacatgggtt gtacggccga agccagcgaa tactatgtag caaacaagat gctcttcgct
     1321 ccgggtaagg ctgttaatgc aggtggtgtt tcttgctcag gtctcgaaat gacgcagaac
     1381 gctatgcacc tcgtttggac gaatgaagaa gtggacaagt ggctgcacca gatcatgcaa
     1441 gacatccacg agcagtgcgt tacatacggt aaagacggca actacatcga ctatgtgaag
     1501 ggtgccaata tcgccggctt catgaaggtt gccaaggcta tggtagctca gggcgtttgc
     1561 taatccctga tttcactccc ctgctcatac agagcagata caaaaacgaa aaagcagaga
     1621 cgtatctccc ctccggaggt acgtctcttt ttatttccac aaacgctact gcgtgactct
     1681 atagctgctt tttattcaaa atttatatcc tacctcctcg attgtcgtta catttggaga
     1741 gattcattct aaattcaata gtaataatat gaagaagaga aaacagctat cgctggcgtt
     1801 ggcatggtcg cttgctttgc ttccggtggg aggatacacg gcatttgccc aagtaaacac
     1861 gacagctcaa acggtgaaac ctcaaaatat aaacccgatg caaaaacgta tgtcctcctt
     1921 tcggcaggaa atgctcagtg aactgacaga tcc
//