From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Brassica napa linoleate desaturase (fad3) mRNA, complete cds.
Date: 3 Jan 93 22:25:47 GMT
Distribution: bionet
Checksum: 35029 4

LOCUS       BNALINDES    1353 bp ss-mRNA            PLN       24-DEC-1992
DEFINITION  Brassica napa linoleate desaturase (fad3) mRNA, complete cds.
ACCESSION   L01418
KEYWORDS    endoplasmic reticulum isozyme; linoleate desaturase;
            linoleic acid desaturase; omega-3-fatty acid desaturase.
SOURCE      Brassica napus Seed, storage deposition stage seed cDNA to mRNA.
  ORGANISM  Brassica napus
            Eukaryota; Plantae; Embryobionta; Magnoliophyta; Magnoliopsida;
            Dilleniidae; Capparales; Brassicaceae.
REFERENCE   1  (bases 1 to 1353)
  AUTHORS   Arondel,V., Lemieux,B., Hwang,I., Gibson,S., Goodman,H.M. and
            Somerville,C.R.
  TITLE     Map-based cloning of a gene controlling omega-3 fatty acid
            desaturation in Arabidopsis
  JOURNAL   Science 258, 1353-1355 (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     polyA_signal    1353
                     /gene="fad3"
     CDS             87..1238
                     /function="desaturation of linoleic acid to linolenic
                     acid"
                     /standard_name="linoleate desaturase"
                     /gene="fad3"
                     /evidence="EXPERIMENTAL"
                     /citation=[1]
                     /product="linoleic acid desaturase"
                     /codon_start=1
                     /translation="MVVAMDQRSNVNGDSGARKEEGFDPSAQPPFKIGDIRAAIPKHC
                     WVKSPLRSMSYVTRDIFAVAALAMAAVYFDSWFLWPLYWVAQGTLFWAIFVLGHDCGH
                     GSFSDIPLLNSVVGHILHSFILVPYHGWRISHRTHHQNHGHVENDESWVPLPEKLYKN
                     LPHSTRMLRYTVPLPMLAYPIYLWYRSPGKEGSHFNPYSSLFAPSERKLIATSTTCWS
                     IMLATLVYLSFLVDPVTVLKVYGVPYIIFVMWLDAVTYLHHHGHDEKLPWYRGKEWSY
                     LRGGLTTIDRDYGIFNNIHHDIGTHVIHHLFPQIPHYHLVDATRAAKHVLGRYYREPK
                     TSGAIPIHLVESLVASIKKDHYVSDTGDIVFYETDPDLYVYASDKSKIN"
BASE COUNT      350 a    319 c    286 g    398 t
ORIGIN      
        1 aatccatcaa acctttattc accacatttc actgaaaggc cacacatcta gagagagaaa
       61 cttcgtccaa atctctctct ccagcgatgg ttgttgctat ggaccagcgc agcaatgtta
      121 acggagattc cggtgcccgg aaggaagaag ggtttgatcc aagcgcacaa ccaccgttta
      181 agatcggaga tataagggcg gcgattccta agcattgctg ggtgaagagt cctttgagat
      241 ctatgagcta cgtcaccaga gacattttcg ccgtcgcggc tctggccatg gccgccgtgt
      301 attttgatag ctggttcctc tggccactct actgggttgc ccaaggaacc cttttctggg
      361 ccatcttcgt tcttggccac gactgtggac atgggagttt ctcagacatt cctctgctga
      421 acagtgtggt tggtcacatt cttcattcat tcatcctcgt tccttaccat ggttggagaa
      481 taagccatcg gacacaccac cagaaccatg gccatgttga aaacgacgag tcttgggttc
      541 cgttgccaga aaagttgtac aagaacttgc cccatagtac tcggatgctc agatacactg
      601 tccctctgcc catgctcgct tacccgatct atctgtggta cagaagtcct ggaaaagaag
      661 ggtcacattt taacccatac agtagtttat ttgctccaag cgagaggaag cttattgcaa
      721 cttcaactac ttgctggtcc ataatgttgg ccactcttgt ttatctatcg ttcctcgttg
      781 atccagtcac agttctcaaa gtctatggcg ttccttacat tatctttgtg atgtggttgg
      841 acgctgtcac gtacttgcat catcatggtc acgatgagaa gttgccttgg tacagaggca
      901 aggaatggag ttatttacgt ggaggattaa caactattga tagagattac ggaatcttca
      961 acaacatcca tcacgacatt ggaactcacg tgatccatca tcttttccca caaatccctc
     1021 actatcactt ggtcgatgcc acgagagcag ctaaacatgt gttaggaaga tactacagag
     1081 agccgaagac gtcaggagca ataccgattc acttggtgga gagtttggtc gcaagtatta
     1141 aaaaagatca ttacgtcagt gacactggtg atattgtctt ctacgagaca gatccagatc
     1201 tctacgttta tgcttctgac aaatctaaaa tcaattaact tttcttccta gctctattag
     1261 gaataaacac tccttctctt ttacttattt gtttctgctt taagtttaaa atgtactcgt
     1321 gaaacctttt ttttattaat gtatttacgt tac
//