From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Microbispora bispora cellodextrinase (bglA) gene, complete cds.
Date: 7 Jan 93 05:52:28 GMT
Distribution: bionet
Checksum: 00578 8

LOCUS       MIBBGLA      3955 bp ds-DNA             BCT       06-JAN-1993
DEFINITION  Microbispora bispora cellodextrinase (bglA) gene, complete cds.
ACCESSION   L06134
KEYWORDS    aryl-beta-D-glucosidase; beta-glucosidase A; cellodextrinase.
SOURCE      Microbispora bispora (library: NRLL 15568) DNA.
  ORGANISM  Microbispora bispora
            Prokaryotae; Firmicutes; Thallobacteria; Actinomycetes and related
            organisms; Maduromycetes; Streptosporangiaceae.
REFERENCE   1  (bases 1 to 3955)
  AUTHORS   Goyal,A.K. and Eveleigh,D.E.
  TITLE     Cloning and characterization of cellodextrinase from Microbispora
            bispora into E.coli
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     misc_feature    345..349
                     /note="This feature applies to a gene which lacks a coding
                     region feature. "
                     /gene="bglA"
     RBS             345..349
                     /gene="bglA"
                     /note="based on similarity; putative"
     CDS             354..3314
                     /function="aryl-beta-D-glucosidase'"
                     /EC_number="3.2.1.21"
                     /standard_name="beta-glucosidase-A'"
                     /gene="bgl-A"
                     /evidence="EXPERIMENTAL"
                     /note="'Hydrolyses beta-1,4-glucosidic bonds e.g. in
                     cellobiose, cellotrioseetc.'; 'Similar to family 3 of
                     glycosyl-hydrolases (contains fungal &bacterial
                     beta-glucosidases)'"
                     /product="cellodextrinase"
                     /codon_start=1
                     /translation="MPLPRPLRAMPLRRPLRRAPRVTLVLALTAAPARADADLPFRDP
                     TLPLAQRIDDLLGRLTLDEKISLLHQYQPPIERLGIKSFKTGTEALHGVAWSTDVTDN
                     GAVVTANGTVFPQAVGLASTWDPELNQRVGTVGEEARGFHAQNPVVWGLNLWAPVVNL
                     LRDPRWGRNEEGYSEDPLLTGAIAIAYGSGIQGDDPDHLRAAPTLKHYLANNNEIRRD
                     TTSSNLPPRVKHEYYEAPFRAAITAGAATGVMTAYNLVNGRPATVNPDLNDTVRTWTD
                     RDLLNVTDAGAPNNLVGSQAYFATLAEADAAALKAGIDSFTTDETNSAPTITAIKTAL
                     SQGLLTEQDIDTAVRHILGIRFRLGEFDPDGGPYAKITPDVIDSPAHRRLARETAAKA
                     MVLLKNERGTLPLDPGKKVAVVGPLADVLYTDWYSGRPTYQVTPLDGIRERAASVTSS
                     EGVDRVAFKDLATGQVHHRGERPEGADLRLSATTIGETEQFDVFDWGQGIVTLRSVAN
                     GKYVSRANWSTLVNNADQPSGWFVQEQFKLEEQDDGSYLIRYAGYETAYDWFGPNTYV
                     KAAPDGTLTLTTAGDATRFAKEVVRSGIDDAVAKAKEADVAVVVVGSMPFINGREDHD
                     RTDMNLAEGQEALVKAVFNANPRTVVVLENSYPTTINWIDEHVPAILWTTHAGAETGN
                     ALADVLYGDVNPAGRLTQTWYLGGRLPDILDYDIVQRDRTYLYFKGTPLYPFGHGLSY
                     TTFRYQGLRVAEKGDAYEVSVRVTNTGHRAGDEVVQVYTHQRTSRVKQPVKQLRAFQR
                     ITLAPGQSKTVTFTIRKADLALWDVTRNKWTVETSAHDVMVGASSADIRQRATIHVKG
                     ERIPDRDLSVPTRAIDFDGYSGVELVDETKARGDAVAGSTGDWIVFKDVDLKRRPSRV
                     TAGVASTSGGSIELRLGSPKGKLIATVPVAATGDVYRYETAAARVTGPSGVKDLYLVF
                     QGDVRIKDLSLTSG"
BASE COUNT      640 a   1472 c   1335 g    508 t
ORIGIN      
        1 ggcgcggcgc tcgacggcgc ggggaggtgg cgggcgctgt ggcggatcgt cgtgccgatc
       61 agccggccca ccctcaccgt gctgctcatc ttcttcttca tctggacctg gaacgagttc
      121 ttcctgccgc tgatctttct gatctccaac gagaaccaga ccgtgccggt cgtgctcggc
      181 gtcctgcaag gggagaagat gatggacgcc accatgtcag cctcgcgctg ctcggcatcc
      241 tcccggccgt cgttcttctt tcctgatctt ccagcgcacg ctgacccgcg gcatcacggt
      301 cggtgcgatc aagtgagccc gcacgcgtcc ctcacgcctg acctggaggc gcaatgccgt
      361 tacctaggcc cctccgcgcg atgccgttac gtaggcccct ccggcgcgct ccccgcgtca
      421 cgctcgtcct cgccctgacg gccgcccccg cgcgagccga cgccgacctt cccttccgcg
      481 accccacgct cccgctcgcc cagcggatcg acgacctgct cggcaggctg acgctcgacg
      541 agaagatctc cctcctccac cagtaccagc cgccgatcga gcgcctcggc atcaagagct
      601 tcaagaccgg caccgaggcg ctgcacgggg tcgcctggtc caccgacgtc accgacaacg
      661 gcgccgtcgt cacggcgaac ggcaccgtgt tcccccaggc ggtcggcctg gcgagcacct
      721 gggacccgga gctgaatcag cgggtcggca ccgtcggcga ggaggcgcgc ggattccacg
      781 cgcagaaccc ggtggtctgg gggctcaacc tgtgggcccc ggtcgtcaac ctgctgcgcg
      841 acccgcgctg ggggcgcaac gaggaggggt actccgagga ccccctgctc accggcgcga
      901 tcgccatcgc gtacggctcg ggcatccagg gggacgaccc cgaccacctc agggcggccc
      961 ccacgctcaa gcactacctc gccaacaaca acgagatccg ccgcgacacc acctcgtcga
     1021 acctgccgcc ccgggtcaag cacgagtact acgaggcgcc gttccgggcg gcgatcacgg
     1081 ccggcgcggc caccggtgtc atgaccgcgt acaacctggt gaacggccgg ccggccacgg
     1141 tcaaccccga cctcaacgac accgtccgga cctggaccga ccgggacctg ctcaacgtca
     1201 ccgacgccgg ggcgcccaac aacctcgtcg gctcgcaggc ctacttcgcc accctggccg
     1261 aggccgacgc cgccgcgctc aaggcgggga tcgacagctt caccacggac gagacgaaca
     1321 gcgcccccac gatcaccgcg atcaagacgg cgctgtcgca gggcctgctc accgaacagg
     1381 acatcgacac cgccgtccgg cacatcctcg gcatccgctt ccggctgggc gagttcgacc
     1441 cggacggcgg gccgtacgcg aagatcaccc cggatgtgat cgacagcccg gcccaccgga
     1501 ggctcgcccg cgagaccgcg gccaaggcca tggtgctgct gaagaacgag cgcggcaccc
     1561 tgccgctcga ccccgggaag aaggtcgcgg tggtcgggcc gctcgccgac gtgctctaca
     1621 ccgactggta ctcgggcagg ccgacgtacc aggtcacccc gctcgacggc atccgggagc
     1681 gggcggcctc ggtgacctcc tcggagggcg tcgaccgggt cgcgttcaag gacctcgcca
     1741 ccgggcaggt acatcaccgc ggggagcgcc ccgagggcgc cgacctcagg ctgagcgcca
     1801 ccacgatcgg cgagaccgag cagttcgacg tgttcgactg gggccagggc atcgtgacgc
     1861 tgcgcagcgt cgccaacggc aagtacgtca gccgggccaa ctggtcgacg ctggtcaaca
     1921 acgccgacca gccgagcggc tggttcgtgc aggagcagtt caagctggag gagcaggacg
     1981 acggctccta cctgatcagg tacgccgggt acgagaccgc ctacgactgg ttcggcccga
     2041 acacctacgt caaggccgcc cccgacggca cgctcaccct caccacggcc ggcgacgcca
     2101 cgcggttcgc caaggaggtg gtccgcagcg gcatcgacga cgcggtcgcc aaggcgaagg
     2161 aggccgacgt ggcggtggtc gtcgtcggca gcatgccgtt catcaacggc cgcgaggacc
     2221 acgaccgcac cgacatgaac ctggccgagg gccaggaggc gctggtcaag gcggtgttca
     2281 acgccaaccc ccgcaccgtc gtcgtgctgg agaacagcta cccgacgacg atcaactgga
     2341 tcgacgagca cgtgccggcc atcctctgga ccacccacgc cggggcggag accgggaacg
     2401 cgctcgccga cgtgctctac ggcgacgtca acccggccgg gcggctcacc cagacctggt
     2461 acctcggcgg acgactgccg gacatcctcg actacgacat cgtgcagcgg gaccggacct
     2521 acctgtactt caagggcacc ccgctctacc ccttcgggca cggcctgtcg tacacgacct
     2581 tccgctacca gggcctgcgg gtggccgaga agggcgacgc ctacgaggtc tcggtacggg
     2641 tgaccaacac cggccaccgg gcgggcgacg aggtcgtcca ggtctacacc caccagcgga
     2701 cctcgcgggt gaagcagccg gtcaagcagc tccgcgcctt ccagcggatc acgctcgccc
     2761 cgggccagag caagaccgtg accttcacca tccgcaaggc cgacctggcg ctgtgggacg
     2821 tgacccggaa caagtggacg gtcgagacct ccgcccacga cgtcatggtc ggggcgtcct
     2881 cggccgacat ccggcagcgg gccacgatcc acgtcaaggg tgagcggatc cccgaccggg
     2941 acctgagcgt ccccacccgg gcgatcgact tcgacggcta ttcgggcgtc gagctggtgg
     3001 acgagaccaa ggcgcgcggg gacgccgtgg cgggctccac cggtgactgg atcgtcttca
     3061 aggacgtcga cctcaagcgg cggccgtccc gggtgaccgc cggggtcgcc tccacctccg
     3121 gcggctccat cgagctccgc ctcggctcgc ccaagggcaa gctgatcgcc accgtgcccg
     3181 tcgccgccac cggcgacgtc taccggtacg agacggcggc cgcccgggtc accgggccgt
     3241 ccggggtcaa ggacctctac ctggtgttcc agggcgacgt gcggatcaag gacctgagcc
     3301 tcacctcggg gtgaccggac gccgcgccgt ccgtacggcc acgccggacc gccggccggg
     3361 ccgcccacca cgcgggcccg tccggaggtc cggcaccggc cgggaggagc ggtccccggt
     3421 gcggccgcgg ggaacgcgcg cgtccgccgg ggccatcgaa gagaagacaa gccgtccggc
     3481 ggccgccgca gggggaggag gagcggcgat ctccgggggg cgcgcaccac gtgcggggac
     3541 gtgcgcgaac gcgggtgatc cagggctgtt tcgctattct ccccgatatg gccgaatcgg
     3601 tgagccagac gctggctcgc ggtatcagct ggctgcgccc gctcggcacc ggcggtatgg
     3661 gcacggtgtg gctggccagg gacacggtgc tcgaccgcga ggctggtcaa ggagctgcgc
     3721 ctcaccgacg gcctctccga ggcggaacgg ccgagctgat ggccagggtc atgcgggagg
     3781 cggaggtgac cgcccgcctc cggcaccccg gcatcgtggc cctgcacgac gtctgatcga
     3841 gggcggccgg ccgtggctcg tcatggagct cctccacggc cgggacctcg cccgggagat
     3901 cgcggccaac ggccgatgcc gccccacgtg gtggccggca tcggcgcccg ggtgc
//