From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Thermus aquaticus DNA for ORF1 and ORF2, complete cds.
Date: 28 Jan 93 05:49:19 GMT
Distribution: bionet
Checksum: 10886 4

LOCUS       TTHORF       1187 bp ds-DNA             BCT       28-JAN-1993
DEFINITION  Thermus aquaticus DNA for ORF1 and ORF2, complete cds.
ACCESSION   D14009
KEYWORDS    ORF1; ORF2.
SOURCE      Thermus aquaticus DNA.
  ORGANISM  Thermus aquaticus
            Prokaryotae; Bacteria; Gracilicutes; Scotobacteria; Neisseriaceae.
REFERENCE   1  (bases 1 to 1187)
  AUTHORS   Utsumi,R.
  TITLE     Cloning and sequence analysis of the thermotolerant gene of Thermus
            aquaticus
  JOURNAL   Unpublished (2048)
  STANDARD  full automatic
COMMENT     Submitted (05-JAN-1993) to DDBJ by: Ryutaro Utsumi Department of
            Agricultural Chemistry Kinki University 3327-204 Nakamachi Nara,
            Nara 631 Japan Phone:  0742-43-1511 Fax:    0742-43-2970.
FEATURES             Location/Qualifiers
     transposon      1..1187
                     /note="the most homologous to IS150, AAAAAAG is found at
                     452 base"
     CDS             192..494
                     /function="DNA binding activity (helix turn helix motif),
                     thermotolerant"
                     /product="ORF1"
                     /codon_start=1
                     /translation="MAKKGQTFNTYSEELKREVVRLKLEEGWSYRQLREHFGIKTDAQ
                     IAQWIKKVQRGESLKDQRGVWNRKHFSSLEEENAYLKAQVEYLKKRNPNLHGKEWS"
     CDS             332..949
                     /note="the most homologous to ORFB of IS150"
                     /product="ORF2"
                     /codon_start=1
                     /translation="MDQESSTRRVIKRSTGSVESQTFFQFGRGKRVFESTGGIPKKAQ
                     SKSTRKGVVLKAERFLIIDELRKKHPLAWLLQIGEVSKAGYYKWHKCRSKRALRLEED
                     LLLKEHLLAIHKIHPYFGYKRMTRALSREGMMVNHKRVRRLMRELGIQSVIQKKRPFY
                     GRRGSVVFPNVLNREFCAGHPFQKLVTDITYVCVWEMRLLTCLRF"
BASE COUNT      401 a    208 c    274 g    304 t
ORIGIN      
        1 aagctttaag gagcttattc atacgatggt agacaaagac cttaagaggt tgaaaaaata
       61 gtatgcaata aaatctagaa aaaaggatat cagatataaa tattctggta ttatccccta
      121 caagtagaca gctgaaaaaa gaggacattt gatatgatgt tcttacgagt ctatttggga
      181 ggggattttt tatggcgaag aaaggacaaa cttttaacac ctattccgaa gagctgaaac
      241 gggaagtcgt tcgtttgaag ttggaagagg gctggtctta tcgtcagctc cgcgaacatt
      301 ttggaatcaa gactgatgcc cagattgcgc aatggatcaa gaaagttcaa cgcggcgagt
      361 cattaaaaga tcaacgggga gtgtggaatc gcaaacattt ttccagtttg gaagaggaaa
      421 acgcgtattt gaaagcacag gtggaatacc taaaaaagcg caatccaaat ctacacggaa
      481 aggagtggtc ttaaaagcag aaaggtttct cattatcgat gagctgcgga aaaagcaccc
      541 acttgcatgg ctgcttcaaa ttggcgaagt ttccaaagct ggatactaca aatggcacaa
      601 atgccgatct aaaagagcat tacgcttgga agaagatctt ctattgaaag aacatttact
      661 cgccattcat aagattcatc cgtacttcgg atataaacga atgacaaggg cattatccag
      721 agaaggaatg atggtaaacc ataaacgtgt acggcgtctc atgagggaat tgggaatcca
      781 atcggtgatt caaaaaaaac gtcctttcta cggacgaaga ggatctgtgg tatttccgaa
      841 tgtcttaaac cgcgaattct gtgccggcca tccgtttcaa aagctcgtaa cagatattac
      901 gtatgtgtgt gtgtgggaga tgcgtttgct tacttgtctg cggttttaga tttatataac
      961 aacgaaattg tcgcgtggga attatcggaa agaaacgatc ttgaattggt attaaatact
     1021 ctgaaacagc tagaggagaa gccatttagc aagaatgctc ttctgcattc ggatcaagga
     1081 tttcaataca cgaccaaatc atatgagaac cagctccaag aattccagat tcaaggaaat
     1141 cattcccgaa gaggaaattg ccatgataat gcatgtatcg aaagctt
//