From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: L.esculentum gene for polyphenol oxidase.
Date: 25 Jul 92 10:07:04 GMT
Distribution: bionet
Checksum: 04930     6

LOCUS       TOMPOOXC     2135 bp ds-DNA             PLN       25-JUL-1992
DEFINITION  L.esculentum gene for polyphenol oxidase.
ACCESSION   Z12835
KEYWORDS    polyphenol oxidase.
SOURCE      Lycopersicon esculentum DNA.
  ORGANISM  Lycopersicon esculentum
            Eukaryota; Plantae; Embryobionta; Magnoliophyta; Magnoliopsida;
            Asteridae; Solanales; Solanaceae.
REFERENCE   1  (bases 1 to 2135)
  AUTHORS   Newman,S.M.
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
REFERENCE   2  (bases 1 to 2135)
  AUTHORS   Newman,S.M., Eannetta,N.T., Yu,H., Prince,J.P., de Vicente,C.,
            Tanksley,S.D. and Steffens,J.C.
  TITLE     Organizationof the tomato polyphenol oxidase gene family
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
COMMENT     *source: strain=cv. VFNT cherry; *source: clone_library=Charon 35
            library of R. Fischer; *source: clone=TGPPO 5; *source: chromosome=
            8; *source: is_macronuclear=N; *source: is_proviral=N; *source:
            is_germline=N;
            From EMBL    entry LEPOOXC;  dated 26-JUN-1992.
FEATURES             Location/Qualifiers
     CAAT_signal     4..9
     TATA_signal     102..106
     CDS             151..1965
                     /product="polyphenol oxidase precursor"
                     /codon_start=1
                     /translation="MASLCSNSSTTSLKTPFTSLGSTPKPCQLFLHGKRNKAFKVSCK
                     VTNTNGNQDETNSVDRRNVLLGLGGLYGVANAIPLAASAAPTPPPDLSSCSIARIDEN
                     QVVSYSCCAPKPDDMEKVPYYKFPSMTKLRVRQPAHEADEEYIAKYNCAVTKMKDLDK
                     TQPDNPIGFKQQANIHCAYCNGGYSIDGKVLQVHNSWLFFPFHRWYLYFYERILGSLI
                     DDPTFGLPFWNWDHPKGMRFPPMFDVPGTALYDERRGDQIHNGNGIDLGYFGDQVETT
                     QLQLMTNNLTLMYRQLVTNSPCPLMSLVDLTLFGSTVEDAGTVENIPHSPVHIWVGTR
                     RGSVLPVGKISNGEDMGNFYSAGLDPLFYCHHSNVDRMWNEWKATGGKRTDIQNKDWL
                     NSEFFFYDENGNPFKVRVRDCLDTKKMGYDYHATATPWRNFKPKTKASAGKVNTGSIP
                     PESQVFPLAKLDKAISFSINRPASSRTQQEKNAQEEVLTFNAIKYDNRDYIRFDVFLN
                     VDNNVNANELDKAEFAGSYTSLPHVHRVGDPKHTATATLRLAITELLEDIGLEDEDTI
                     AVTLVPKKGDISIGGVEIKLQGFTAKFRRIVFTGFFTG"
     transit_peptide 151..439
     mat_peptide     400..1962
                     /product="polyphenol oxidase"
     misc_binding    730..840
                     /bound_moiety="copper, tyrosinase-like CuA bindingsite"
     misc_binding    1120..1278
                     /bound_moiety="copper, tyrosinase-like CuB bindingsite"
BASE COUNT      615 a    432 c    488 g    600 t
ORIGIN
        1 attcaaaatt ccccacctaa cgcgtcctgg aataattgat atgacttgaa gccgcctcta
       61 aaattaaata ataattggtg cttataatgt tttacatatt atataaagca aggtatagcc
      121 caatgaattt tcattcaaaa gctagcaaca atggcaagtt tgtgtagtaa tagtagtact
      181 acttctctca aaactccttt cacttcttta ggttccactc caaagccttg tcaacttttc
      241 ctacatggaa aacgtaacaa agcattcaaa gtttcatgca aggttaccaa tactaacggt
      301 aaccaagatg aaacgaattc tgtagatcga aggaatgttc ttcttggctt aggaggtctt
      361 tatggtgttg caaatgctat accattagct gcatcggctg ctcctacacc acctcctgat
      421 ctctcgtctt gtagtatagc aaggattgac gagaatcagg tggtgtcata cagttgttgc
      481 gcgcctaagc ctgatgatat ggagaaagtt ccgtattaca agttcccttc tatgactaag
      541 ctccgtgttc gtcagcctgc tcatgaggct gatgaggagt acatcgccaa gtacaactgt
      601 gcggttacga agatgaaaga tcttgataag acacaacctg ataaccctat tggttttaag
      661 caacaagcta atatacattg tgcttattgt aatggtggtt attctattga tggcaaagtg
      721 ttgcaagttc ataactcgtg gcttttcttc ccgttccata gatggtactt gtacttctac
      781 gaaagaatct tgggatcact catcgatgat ccaacattcg gtttgccatt ttggaattgg
      841 gatcatccaa agggcatgcg tttccctccc atgtttgatg ttccagggac cgccctttat
      901 gacgaaaggc gtggtgatca aatccataat gggaacggga tagatcttgg ttattttggg
      961 gatcaagttg agacaactca gttgcagttg atgactaata acttaaccct gatgtaccgt
     1021 cagttggtaa ctaattctcc atgtcctctt atgtctttgg tggaccttac actctttggg
     1081 agtacggtcg aagacgcggg aactgttgaa aacatccctc acagccctgt tcacatttgg
     1141 gttggtacga gacgaggttc agttttacct gtaggtaaaa tatcaaatgg tgaggatatg
     1201 ggtaatttct attcagctgg tttagacccg cttttctatt gccatcacag caatgtggac
     1261 aggatgtgga acgaatggaa ggcaactgga ggtaaacgaa cagatataca aaacaaagat
     1321 tggttgaact cggagttctt tttctatgat gagaatggaa atccgttcaa agtgagagtc
     1381 agagactgtt tggatacgaa gaagatgggg tatgattacc acgcaacggc gaccccatgg
     1441 cgtaacttca agccaaaaac aaaggcttca gctgggaaag tgaatacggg ttcaattcct
     1501 cccgaaagcc aggtattccc attggctaaa ctcgacaaag caatttcatt ttccatcaac
     1561 aggcctgctt cctcgaggac tcaacaagag aaaaatgcac aagaggaggt cctaacattc
     1621 aatgcaataa aatatgataa cagagattac ataagattcg atgtgttcct caacgtggat
     1681 aacaatgtga acgcgaatga gcttgacaag gcagagtttg cggggagtta tactagcttg
     1741 ccacatgtac acagagttgg agatcctaaa catactgcaa ctgctactct ccggctggcg
     1801 ataacagaac tgttggagga cattggtttg gaagatgaag atactatcgc ggtgactctg
     1861 gtaccaaaga aaggtgatat ctccattgga ggtgtggaga tcaagcttca gggcttcact
     1921 gcgaaattca ggcgaatcgt gtttacgggg ttttttactg gttgatactg tttttgtcat
     1981 gtgagtcacc tctgactgag agtttactca cttagccgcg tgtccactat tgctgggtaa
     2041 gatcaaacgg ccagggcaac acccagagta cccgggatga agaacggatg gttgaagacg
     2101 aatttaccga tacggtagta cgccgtcatc catct
//