From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: M.musculus HSP47 mRNA
Date: 28 Jul 92 10:06:42 GMT
Distribution: bionet
Checksum: 04094     5

LOCUS       MUSHSP47     2273 bp ss-RNA             ROD       28-JUL-1992
DEFINITION  M.musculus HSP47 mRNA
ACCESSION   X60676
KEYWORDS    ER protein; HSP47 gene; collagen binding; heat shock protein.
SOURCE      Mus musculus RNA.
  ORGANISM  Mus musculus
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Rodentia; Myomorpha; Muridae; Murinae.
REFERENCE   1  (bases 1 to 2273)
  AUTHORS   Takechi,H., Hirayoshi,K., Nakai,A., Kudo,H., Saga,S., Kita,T. and
            Nagata,K.
  TITLE     Molecular cloning of a mouse 47-kDa head-shock protein (HSP47), a
            collagen-binding stress protein, and its expression during the
            differentiation of F9 teratocarcinoma cells
  JOURNAL   Eur. J. Biochem. 206, 323-329 (1992)
  STANDARD  full automatic
COMMENT     *source: strain=balb/c; *source: cell_line=balb/c 3T3 fibroblast;
            *source: clone_library=CDM8MHSP; *source: clone=pMH471;
            From EMBL    entry MMHSP47;  dated 29-JUN-1992.
FEATURES             Location/Qualifiers
     sig_peptide     257..307
     CDS             257..1510
                     /product="heat shock protein"
                     /gene="HSP47"
                     /codon_start=1
                     /translation="MRSLLLGTLCLLAVALAAEVKKPLEAAAPGTAEKLSSKATTLAE
                     RSTGLAFSLYQAMAKDQAVENILLSPLVVASSLGLVSLGGKATTASQAKAVLSAEKLR
                     DEEVHTGLGELLRSLSNSTARNVTWKLGSRLYGPSSVSFADDFVRSSKQHYNCEHSKI
                     NFRDKRSALQSINEWASQTTDGKLPEVTKDVERTDGALLVNAMFFKPHWDERFHHRMV
                     DNRGFMVTRSYTVGVTMMHRTGLYNYYDDEKEKLQMVEMPLAHKLSSLIILMPHHVEP
                     LERLEKLLTKEQLKAWMGKMQKKAVAISLPKGVVEVTHDLQKHLAGLGLTEAIDKNKA
                     DLSRMSGKKDLYLASVFHATAFEWDTEGNPFDQDIYGREELRSPKLFYADHPFIFLVR
                     DNQSGSLLFIGRLVRPKGDKMRDEL"
     mat_peptide     308..1507
                     /product="heat shock protein"
                     /gene="HSP47"
     misc_signal     1496..1507
                     /note="ER retention signal"
     polyA_site      2252
BASE COUNT      519 a    619 c    659 g    476 t
ORIGIN
        1 gagcgattgc cctgtcggtc ccggagcagc ccagcccggc ccaggtgagg ggcgggacgg
       61 ccgggcgatt tggggttgcg cattggccaa gggggaacgg atcgtccaaa gcttggggtc
      121 ctgacacagg tgggcagtga aagatcggac ttggaactgt cacttgcctg ggttccctgg
      181 agctgcggtc tagtacgcgg actgcctggt aaaccctcac aggtcctctg tggtgcacac
      241 agcccacctc ccagccatgc gctctctcct tctgggcacc ttatgcctct tggccgtggc
      301 cctggcagcc gaggtgaaga aacccctaga ggcggcagcc cctggtactg cggagaagct
      361 aagttccaag gcgaccacac tggcagagcg cagcacaggc ctggccttca gcctatatca
      421 ggcgatggcc aaagaccagg cggtggagaa catcctcctg tcacccttgg tggtggcctc
      481 atccctgggt cttgtgtcac tgggtggtaa agccaccaca gcgtcgcagg cgaaggcagt
      541 gctgagcgct gagaagctgc gcgatgagga ggtgcacacg gggctgggtg agctgctccg
      601 ctccctcagc aactccactg cgcgcaacgt gacctggaaa ctgggcagcc gcctgtacgg
      661 gcccagctcc gtgagcttcg ccgatgactt cgtgcgcagc agcaagcaac actacaactg
      721 cgaacactcc aagatcaact tccgagacaa gcgcagcgcc ctgcagtcca tcaacgagtg
      781 ggcctcgcag accacggacg gcaagctgcc tgaggtcacc aaggatgtgg agcgcacgga
      841 tggggcactg cttgtgaacg ccatgttctt taagccacac tgggatgaga ggtttcacca
      901 caggatggtg gacaaccgtg gcttcatggt gacccgctcc tatactgtgg gtgttacgat
      961 gatgcaccgg acaggcctgt acaactacta tgacgacgag aaggagaagc tgcagatggt
     1021 ggagatgccc ctggctcaca agctctccag cctcatcatc ctcatgcccc accatgtgga
     1081 gccgctcgag cgcttggaga agctgctgac caaggagcag ctgaaggcct ggatgggaaa
     1141 gatgcagaag aaggctgtcg ccatctccct gcccaagggc gtggtggagg tgacccatga
     1201 cctgcagaaa catctggcag gactgggcct gaccgaagcc atcgacaaga acaaggcaga
     1261 cctatcgcgc atgtctggca agaaggacct gtacctggcc agtgtgttcc acgccactgc
     1321 cttcgagtgg gacaccgagg gcaacccctt tgaccaagac atctacgggc gcgaggagct
     1381 gcgcagcccc aagctgttct atgccgacca ccccttcatc ttcctggtgc gagataatca
     1441 gagcggctcc ttgctcttca ttggccgcct ggtccggccc aagggagaca agatgcgaga
     1501 tgagttgtag agtccaagag tgggcgtggc acggcaggaa gtagccaaag gttcctgaga
     1561 cacatgggtg ctattgtggg gggaggggga ggcaccaacc ctggatattc cacgggtggg
     1621 ggggggttgg cagtgcaaac cggaattccc atatgtctga gtgggtattt ttagacagaa
     1681 tccactccta agttaggaca tggagcccag atactatgat accaaattca ggggtgtcac
     1741 agccattttg ttctgccctg caagttttag atccaatctg cctcaacagt caatcagtgt
     1801 tcatatttat ggccaggcat tttatctgtt agacaatcga gttgggggta gggcagccta
     1861 gctctttttg tcaccatgcc ccgagccctc ctcagtcttc ctgttcaccc ctcccccagc
     1921 tccctttaac cgcaaaacta ggtgctgtgg ccccagaact gcccatcctt aaaatgatcc
     1981 ttgcccagcg gtgggagctg gagacagcgc atggaggggg ctccctggca tacctgccct
     2041 agaactgtta tgttgggcat cacagtcatg aacttttgtt tttctccctt ttttagtttt
     2101 ttcaaagatg gggggagggg aaatatgagc ctttgttgct ttcaaaacga gaacagtttg
     2161 tacaagtttt tttttgaata aaacttttcc aatgaaatgt tacaggtgtg tgaaagcaga
     2221 ttaaagaaag aggttcccat atcattcttt ctaaaaaaaa aaaaaaaaaa aaa
//