From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: acvA=acv synthetase [Aspergillus nidulans, Genomic, 2057 nt,
Date: 29 Dec 92 01:28:19 GMT
Distribution: bionet
Checksum: 55185 6

LOCUS       M132910S2    2057 bp    DNA             NLM       08-DEC-1992
DEFINITION  acvA=acv synthetase [Aspergillus nidulans, Genomic, 2057 nt,
            segment 2 of 2] 
ACCESSION   S40247 X54853
KEYWORDS    .
SEGMENT     2 of 2
SOURCE      Aspergillus nidulans
  ORGANISM  Aspergillus nidulans
            Unclassified.
REFERENCE   1  (bases 1 to 2057)
  AUTHORS   MacCabe,A.P., van Liempt,H., Palissa,H., Unkles,S.E., Riach,M.B.,
            Pfeifer,E., von Dohren,H. and Kinghorn,J.R.
  TITLE     Delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine synthetase from
            Aspergillus nidulans. Molecular characterization of the acvA gene
            encoding the first enzyme of the penicillin biosynthetic pathway.
  JOURNAL   J. Biol. Chem. 266, 12646-12654 (1991)
  STANDARD  full automatic
COMMENT     This entry [NCBI gibbsq 40247] was created by the journal scanning
            component of NCBI/GenBank at the National Library of Medicine.
            sixty bases from 9386 to 9445 are missing from the figure.
            This sequence comes from Fig. 2.  The authors begin their numbering
            at 9446.
FEATURES             Location/Qualifiers
     CDS             3..1865
                     /note="the first codon is short of one base due the
                     missing sequences.  For the protein sequence (NCBI gibbsq
                     40272):  Method: conceptual translation with partial
                     peptide sequencing.  20 a.a. gap from 3129 to 3148 are
                     missing.
                     This sequence comes from Fig. 2.  The authors begin their
                     numbering at 3149.
                     Author-given protein sequence differs from the conceptual
                     translation for reasons explained in citation"
                     /codon_start=1
                     /translation="VAELQLYHDAFRMRLRQIDGRTVQCFADDISPVQLRVLNVKDVD
                     GSAAIDQQLQKYQSDFDLEKGPICAAAYLHGYEDRSARVWFSVHHIIIDIVSWQILAR
                     DLQILYEGGTLGRKSSSVRQWAEALQSYQGSASERAYWEGLLAQTAANISALPPVTGT
                     RTRLARTWSDDRTVILLNEASNQNASIQDLLLAAVGLALQQVTPGSPSMITLEGHGRE
                     EIVDPTLDLSRTLGWFTSMYPFEIPPLNVETLSQGIASLRECLRQVPARGIGFGSLYG
                     YCKHQMPQVTFNYLGQLTSKQSITDQWALAVGDGEMQYGLTTSPADRDQSSFAVDITA
                     SCVNGALSVEMNSAWSLEKSMRFISRIEEVLNMILSGTLAQQATPVLTPQVFNEEMYT
                     PYFEFSKTPRRGPILFLLPPGEGGAESYFNNIVKHLPTTNMVVFNNYYLHSKSLNTFE
                     KLAEMYLGHIRQIQPDGPYHFIGWSFGGTIAMEISRQLVGLGSTIGLLGIIDTYFNVP
                     GATRAIGLGDTEVLDPIHHISQPEPADFQCLPASTDYIILFKATRVNDKFQSENQRRL
                     YEYYDKTLLNDLDWLLPGASNIHLVRLEEDTHFSWATNPRQIAHVCSTIEKFLARY"
BASE COUNT      541 a    519 c    505 g    492 t
ORIGIN      
        1 tcgccgaatt gcagctgtat catgacgcct tcagaatgcg gttgaggcaa atagatggaa
       61 ggacggtgca atgcttcgca gatgacattt ctccagtaca gctccgagtg ttgaacgtca
      121 aggatgtcga cggaagcgcg gctattgacc agcaactcca gaaatatcag tctgacttcg
      181 accttgagaa aggcccaatc tgtgctgctg cctacctcca tggctacgag gatcgatctg
      241 cacgagtctg gttttctgtc caccacatca tcattgatat agttagctgg cagattcttg
      301 cgcgcgacct acaaatcctg tacgagggtg gaactctcgg tcgtaagagt agcagcgtca
      361 gacaatgggc agaggcacta cagagctacc aggggtcggc atcggagagg gcctactggg
      421 aaggacttct tgctcaaacg gctgccaaca tatccgcttt gcccccagtg accgggaccc
      481 gtacccggtt ggctcgaact tggagtgacg acaggacggt cattctcctg aatgaagctt
      541 ctaatcagaa tgcatctata caagacctct tactcgccgc tgttggattg gcacttcaac
      601 aggtcacccc gggtagcccg agtatgatta ctctcgaggg ccatgggcgt gaggaaattg
      661 ttgacccgac attagacctc agccgtacct tgggttggtt caccagcatg tatcccttcg
      721 agatccctcc cctgaatgtt gaaaccctta gccagggcat agccagcttg cgagaatgcc
      781 ttaggcaggt gcctgcacgg ggcatcgggt ttggatcact ctacggttat tgcaaacacc
      841 aaatgcctca ggttacgttc aactacctgg gccagctgac aagcaagcaa tcgataactg
      901 atcagtgggc cctcgctgtt ggtgacggag agatgcaata tgggcttaca acaagtcctg
      961 cggacagaga ccaaagctcg ttcgcggttg atatcaccgc cagctgtgta aatggtgccc
     1021 tgtcagtcga aatgaatagt gcctggagcc ttgaaaaaag catgcgattc atatccagga
     1081 ttgaggaagt attgaatatg attcttagcg ggaccctagc tcagcaggcg actccagtgc
     1141 ttacgccaca ggtattcaac gaggagatgt acacaccata ttttgaattt tccaaaaccc
     1201 cacgacgcgg accgatcttg ttcctattgc cgccagggga gggaggggca gaaagctact
     1261 ttaacaatat cgtcaagcac ttgcccacga ctaatatggt cgtctttaac aattactacc
     1321 ttcactccaa gagtctgaac acgtttgaaa agctagctga gatgtatttg gggcacatcc
     1381 gtcagatcca gccagacggg ccttaccatt tcatcggatg gagttttgga ggaacaatcg
     1441 cgatggaaat atcgcgacag ctcgtggggc taggttcaac gattggtctt ttaggtatca
     1501 ttgacacgta tttcaacgtg cctggagcaa cgcgggcaat tggcctcggt gatactgagg
     1561 tcttggatcc cattcatcat atatcccaac cagaaccagc cgatttccag tgcctcccag
     1621 ccagcacaga ctacatcatt ttattcaaag ctactagggt gaacgacaag tttcagtctg
     1681 aaaaccagag gcgtctgtac gagtactacg acaaaacatt gcttaatgat ctcgactggt
     1741 tactccctgg tgcttcaaac attcatctag tccgccttga ggaggatact cacttctcct
     1801 gggcgaccaa tccacgccaa atcgcccacg tttgttcaac aatcgagaaa tttctcgcca
     1861 gatattagta aagtgatgcc aacaacgtat gcataatgag gtggatgtaa tccagcaagg
     1921 tcaggttcgg ggcaggattt tgtgctgtaa ctcactttac gtggatatat atctttagag
     1981 agacaataat caatcatact tcaaataatg aaacgaacct gctcattgca aaacatgcct
     2041 aagcagcaca gcaaccc
//