From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: B.firmus ORF A and ORF B, complete cds.
Date: 10 Sep 92 10:13:58 GMT
Distribution: bionet
Checksum: 60304     7

LOCUS       BACKATA2     3263 bp ds-DNA             BCT       10-SEP-1992
DEFINITION  B.firmus ORF A and ORF B, complete cds.
ACCESSION   L02548 M74194
KEYWORDS    .
SEGMENT     2 of 2
SOURCE      Bacillus firmus DNA.
  ORGANISM  Bacillus firmus
            Prokaryotae; Firmicutes; Endospore-forming rods and cocci;
            Bacillaceae.
REFERENCE   1  (bases 1 to 3263)
  AUTHORS   Quirk,P.G. and Krulwich,T.A.
  JOURNAL   Unpublished (1991)
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     RBS             210..219
     CDS             225..1616
                     /note="ORF A"
                     /note="putative"
                     /codon_start=1
                     /translation="MELEKKEILVSEFEREETMTTTNKLKFIIPSIVGLFLFLIPLNY
                     GGKWTIGVGILAETAQGITADYLPAFMVAVLLLSVVLTIAANVAKPQWIMNSAFLKGL
                     FHVSGFWLVMRAAGALFAVMVIFEIGPAFIWDAYTGGTVLYELVPVLTKWFLFAGLLM
                     PLLLEFGLMDFIGTSLRKVMRPLFRLPGRSSIDAVASWMGSGTVGVLITTKQYEEGFY
                     TKREAAVIATNFSVASIAFSLVVISFIGLDYMFIPFYLTVIVAGLIAAIICPRIPPLS
                     RKADTYYEGAGKQISEEVPEGKTSRQWALERAVATASKVKSVKGVATKGFQNVLDIWF
                     GLIPLVMALGTIALIVAEYTSIFTYLSYPFVPILELLRIPEAQAAAPALLVGFADMFL
                     PAVVGSGIESELTRFVIAAVSMTQLIYMSEIGILLLRSKIPLSLLDLVIVFFQRTIIT
                     LPIIVFMAHVFFF"
     RBS             1643..1648
     CDS             1657..2994
                     /note="ORF B"
                     /note="putative"
                     /codon_start=1
                     /translation="MSADWSYLREKSASRLAPSMAKDHPNLPVVKEEGCYYYGVDGVK
                     YLDFTSGIAVTNVGHRHPKIVQAIKEAADHLTHGPIGVIQYESILKLADELADILPGD
                     LDCFFFANSGTEAIEGALKLAKFVTKRPYVVSFTGCFHGRTQGSLGVSTSKSKYRKFL
                     QPNGLTYQVPYFKPSDPRILDEEGEVVESLACELLEEEFTNLFKYHVSSEEVAAVILE
                     PVLGEGGYIIPPASWLAKVREICNRHDILLIFDEVQTGFGRTGEWFAAQTFGVTPDIM
                     AIAKGIASGLPLSATVANHTLMQQWPLGSHGTTFGGNPIACSAALATLDVLKEENLLD
                     NAREVGAYARERLNLLKEKYEMIGSIRSVGLMIGIEIIDPQTKKPDGAAVLRILDLAL
                     QEGVLFYLCGNEGEVIRMIPPLSVTKEQIDDGLDMLQRALVKYKEETHQPASL"
BASE COUNT      942 a    577 c    774 g    970 t
ORIGIN
        1 gatccttgaa ggagtcgaga gagaggttct ccaaaaagct aaaactcaat ataggaccac
       61 tgttgagatg gcaaaatatt taggtgtcag tcaacctaca atagtcagaa aattaagcaa
      121 atataaaata aaatgattaa ttggtacatt tattgcatta taaaaagtta cacatctaga
      181 gaagcatgta ggtctaaaac taaagggtaa ggtgaggtgc ttttatggag ttagagaaaa
      241 aggagatttt agtttcagag tttgaacgag aagaaacgat gaccacaacc aacaagttga
      301 aattcattat cccatcaata gtgggcttgt ttctattcct tattccatta aattatggtg
      361 ggaaatggac cattggagtc ggaattttag ctgaaactgc tcagggaata actgctgatt
      421 atctcccagc ctttatggta gctgtgctct tattatcggt tgtattaaca atcgcagcaa
      481 acgtagccaa gcctcaatgg attatgaact ctgcttttct taaaggctta tttcatgtca
      541 gcggcttttg gctggtgatg cgagcagcag gagcattgtt tgccgttatg gtcatttttg
      601 aaataggacc tgcctttatc tgggatgctt atacaggagg gactgttctg tatgaattag
      661 ttcccgtact cacgaagtgg tttctatttg ccggccttct tatgccttta ttactggagt
      721 ttggattaat ggactttatt ggaacttcac ttagaaaagt gatgcgccct ttattccgtc
      781 ttccgggcag gtcctccatt gatgcggttg cttcttggat gggaagcggt acggtagggg
      841 tgcttataac cacaaagcag tatgaggaag gattctatac aaaacgcgaa gcagcggtca
      901 ttgcgacgaa tttctctgtt gcatctatcg cgtttagctt agtcgtcatt agttttatcg
      961 gtcttgatta tatgtttatt ccattttatt tgacggttat cgtggcaggg ttgattgcag
     1021 ccattatctg tcctagaatt ccgccgctct caagaaaagc ggatacgtat tatgagggag
     1081 ctggaaaaca aatatcagaa gaagtgcccg aggggaaaac gagcagacag tgggcgttag
     1141 aaagagctgt agctacagct tcaaaagtga aaagtgtgaa aggtgtagca actaaaggtt
     1201 ttcaaaatgt tttagatatt tggttcgggc tcatcccgct tgtcatggcg ttagggacga
     1261 ttgctttaat tgttgccgaa tatacatcta tctttactta tttatcctat ccttttgtac
     1321 cgatcctaga attacttaga atccctgagg cgcaagctgc tgccccggcg ttacttgtag
     1381 gctttgcaga catgtttttg cctgctgtgg tggggagcgg cattgaaagt gagttaacgc
     1441 gctttgtgat tgcagctgtt tcaatgactc agcttatcta tatgtctgaa attggaattt
     1501 tactgcttcg ttcaaagatt cctttgagcc tgcttgattt agttattgta tttttccagc
     1561 gtacgattat tacattaccg attattgtgt tcatggcgca cgtctttttc ttctaagtga
     1621 acgtacattg attatttatg aaagggagcg aggcaaatga gtgcggattg gagttacctg
     1681 cgagaaaaat cagcttcacg attagcaccc agcatggcaa aagatcaccc taatttacct
     1741 gtagtaaaag aagaaggctg ttattattac ggggtagatg gggtgaaata tttggatttc
     1801 acatcaggaa ttgcagtgac aaatgtggga catcgtcatc cgaagattgt tcaggctatt
     1861 aaggaagcag ctgatcattt aacccatgga ccgattggtg tcattcaata tgaatctatt
     1921 ttaaaattag cagatgagct cgctgatatt ctgcctggtg atcttgattg tttctttttt
     1981 gcaaacagcg ggacagaagc aattgaaggg gcgttaaagt tagctaaatt tgtaacgaag
     2041 cggccttatg tcgtttcatt tacaggatgc tttcatggcc ggactcaagg gtcacttggg
     2101 gtaagcacat ccaaaagcaa gtatcgaaaa tttctgcagc caaatgggtt aacttaccag
     2161 gttccttact ttaaaccgtc tgatccaaga attcttgatg aggaagggga agtcgtagaa
     2221 tctctagcgt gtgagctgct agaagaagag tttacgaacc tatttaaata tcacgtatca
     2281 tctgaggaag tagcagcggt gatcttagag cccgttcttg gagaaggcgg atatataatc
     2341 ccgcctgcct cttggttagc taaggtaagg gagatttgta accgtcacga tatactactg
     2401 atatttgatg aagtccaaac agggtttggc cgcacaggag aatggtttgc ggctcaaact
     2461 ttcggggtca ctccagatat tatggcaatt gctaaaggaa ttgcttcagg tttgccgctt
     2521 agtgcaacgg tagcaaatca cacactgatg cagcaatggc cgcttggaag ccacggaaca
     2581 acctttggag gaaacccgat agcttgttca gctgcattag ccactttaga cgttctaaaa
     2641 gaagaaaatc tattagataa cgccagagaa gtcggtgcct atgcaagaga gaggctcaat
     2701 ttattaaaag agaagtatga gatgattgga agcatccgta gtgtcgggtt aatgattggg
     2761 atagagatca ttgatccaca aacaaagaag ccagacggag ctgcagtgct gaggatatta
     2821 gatttagccc tgcaggaagg tgttttattt tatctatgcg gcaatgaagg agaggtcatt
     2881 cgaatgatcc cgccgcttag cgtgacaaaa gagcaaattg acgatggttt agatatgctt
     2941 cagcgtgcgt tagtaaagta taaagaagaa acgcatcagc cagcatccct ctagtatgcc
     3001 attcatttaa ggaccgcctt ctagttataa ttgttttatc ttgtacttat cgcaaagaca
     3061 tgatatcaat agattataaa actatttacc tatatctatt gtatggtgta aaagtggatg
     3121 aggtggaaga atgagtacgg gtaaaacaga tcaagaaata ttagattcca ttgttaccgc
     3181 agctccattt attcaaaaag cggctaaaga agaaattgcc atatctatat gtgaccatga
     3241 aacatggctg ttgcagctag atc
//