From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Thermomonospora fusca endo-1,4-beta-glucanase gene, complete cds.
Date: 12 Sep 92 10:04:05 GMT
Distribution: bionet
Checksum: 14737     6

LOCUS       THFEBG       2116 bp ds-DNA             BCT       12-SEP-1992
DEFINITION  Thermomonospora fusca endo-1,4-beta-glucanase gene, complete cds.
ACCESSION   L01577
KEYWORDS    1,4-beta-D-glucan 4-glucanohydrolase; cellulase;
            endo-1,4-beta-glucanase.
SOURCE      Thermomonospora fusca (strain YX) DNA.
  ORGANISM  Thermomonospora fusca
            Prokaryotae; Firmicutes; Thallobacteria;
            Actinomycetes and related organisms; Thermomonosporaceae.
REFERENCE   1  (bases 1 to 2116)
  AUTHORS   Lin,E. and Wilson,D.B.
  TITLE     Transcription of the celE gene in Thermomonospora fusca
  JOURNAL   J. Bacteriol. 170, 3838-3842 (1988)
  STANDARD  full staff_review
REFERENCE   2  (bases 1 to 2116)
  AUTHORS   Lin,E. and Wilson,D.B.
  JOURNAL   J. Bacteriol. 170, 3843-3846 (1988)
  STANDARD  full staff_entry
REFERENCE   3  (bases 1 to 2116)
  AUTHORS   Lao,G., Ghangas,G.S., Jung,E.D. and Wilson,D.B.
  TITLE     DNA sequences of three beta-1,4-endoglucanase genes from
            Thermomonospora fusca
  JOURNAL   J. Bacteriol. 173, 3397-3407 (1991)
  STANDARD  full staff_entry
FEATURES             Location/Qualifiers
     promoter        353..379
                     /note="Three potential promoters are located within this
                     region."
                     /citation=[1]
     5'UTR           376..492
                     /citation=[1]
     misc_feature    376
                     /note="alternate initiation site for mRNA"
     misc_feature    380
                     /note="alternate initiation site for mRNA"
     misc_feature    387
                     /note="alternate initiation site for mRNA"
     protein_bind    444..457
                     /bound_moiety="regulatory protein"
                     /evidence=EXPERIMENTAL
                     /citation=[3]
                     /citation=[1]
                     /citation=[2]
     RBS             483..490
                     /note="3' end of 16S RNA of S. lividens"
     sig_peptide     493..600
                     /codon_start=1
     CDS             493..1893
                     /product="endo-1,4-beta-glucanase"
                     /codon_start=1
                     /translation="MAKSPAARKGXPPVAVAVTAALALLIALLSPGVAQAAGLTATVT
                     KESSWDNGYSASVTVRNDTSSTVSQWEVVLTLPGGTTVAQVWNAQHTSSGNSHTFTGV
                     SWNSTIPPGGTASSGFIASGSGEPTHCTINGAPCDEGSEPGGPGGPGTPSPDPGTQPG
                     TGTPVERYGKVQVCGTQLCDEHGNPVQLRGMSTHGIQWFDHCLTDSSLDALAYDWKAD
                     IIRLSMYIQEDGYETNPRGFTDRIDQLIDMATARGLYVIVDWHILTPGDPHYNLDRAK
                     TFFAEIAQRHASKTNVLYEIANEPNGVSWASIKSYAEEVIPVIRQRDPDSVIIVGTRG
                     WSSLGVSEGSGPAEIAANPVNASNIMYAFHFYAASHRDNYLNALREASELFPVFVTEF
                     GTETYTGDGANDFQMADRYIDLMAERKIGWTKWNYSDDFRSGAVFQPGTCASGGPWSG
                     SSLKASGQWVRSKLQS"
     mat_peptide     601..1890
                     /EC_number="3.2.1.4"
                     /product="endo-1,4-beta-glucanase"
                     /codon_start=1
BASE COUNT      359 a    793 c    625 g    338 t      1 others
ORIGIN
        1 gaattccagg atggtcgtgt ggactgcggc caagcggggg tcggtcgacg tgctcggggg
       61 tgatcccgtg gacgcgatct tctcctgctc gaactcctcg acgggggccg ggggacggac
      121 cagggtggtg tagcggtcga cgatccgccc gtcggtgact cgcacgagtc ccaccgcgca
      181 cacgctgccc tggttgcggt tggcggtctc gaagtcgata gcggtccagg tgtgctcgat
      241 cgccggtcgt cctcccttgg gccgcactgg ccaggcgtcg gggttcctcc catgctcccg
      301 gaacttcacc tcatccagca cccctccttc tttgagtgac ctagatcact tattgctctt
      361 gactttgtgc tacggtctca ctacataacg agaacggcgc attcctcctt ccccgattct
      421 gtcacggaat gcgcatccct atatgggagc gctcccatag ccgtcttcct cccctctccc
      481 cttggaggaa ccatggcgaa atcccccgcc gcccggaagg gcngccctcc ggtcgctgtc
      541 gcggtgaccg cggccctcgc cctgctgatc gcgctcctct cccccggagt cgcgcaggcc
      601 gccggtctca ccgccacagt caccaaagaa tcctcgtggg acaacggcta ctccgcgtcc
      661 gtcaccgtcc gcaacgacac ctcgagcacc gtctcccagt gggaggtcgt cctcaccctg
      721 cccggcggca ctacagtggc ccaggtgtgg aacgcccagc acaccagcag cggcaactcc
      781 cacaccttca ccggggtttc ctggaacagc accatcccgc ccggaggcac cgcctcttcc
      841 ggcttcatcg cttccggcag cggcgaaccc acccactgca ccatcaacgg cgccccctgc
      901 gacgaaggct ccgagccggg cggccccggc ggtcccggaa ccccctcccc cgaccccggc
      961 acgcagcccg gcaccggcac cccggtcgag cggtacggca aagtccaggt ctgcggcacc
     1021 cagctctgcg acgagcacgg caacccggtc caactgcgcg gcatgagcac ccacggcatc
     1081 cagtggttcg accactgcct gaccgacagc tcgctggacg ccctggccta cgactggaag
     1141 gccgacatca tccgcctgtc catgtacatc caggaagacg gctacgagac caacccgcgc
     1201 ggcttcaccg accggatcga ccagctcatc gacatggcca cggcgcgcgg cctgtacgtg
     1261 atcgtggact ggcacatcct caccccgggc gatccccact acaacctgga ccgggccaag
     1321 accttcttcg cggaaatcgc ccagcgccac gccagcaaga ccaacgtgct ctacgagatc
     1381 gccaacgaac ccaacggagt gagctgggcc tccatcaaga gctacgccga agaggtcatc
     1441 ccggtgatcc gccagcgcga ccccgactcg gtgatcatcg tgggcacccg cggctggtcg
     1501 tcgctcggcg tctccgaagg ctccggcccc gccgagatcg cggccaaccc ggtcaacgcc
     1561 tccaacatca tgtacgcctt ccacttctac gcggcctcgc accgcgacaa ctacctcaac
     1621 gcgctgcgtg aggcctccga gctgttcccg gtcttcgtca ccgagttcgg caccgagacc
     1681 tacaccggtg acggcgccaa cgacttccag atggccgacc gctacatcga cctgatggcg
     1741 gaacggaaga tcgggtggac caagtggaac tactcggacg acttccgttc cggcgcggtc
     1801 ttccagccgg gcacctgcgc gtccggcggc ccgtggagcg gttcgtcgct gaaggcgtcc
     1861 ggacagtggg tgcggagcaa gctccagtcc tgatgtgcag cgcggtggct gcgcgcccct
     1921 gagcacagca agcagcccgc gaggctcagc cccgcgggcc gcttctctgg gtccttcctc
     1981 ccccgtgggg atgagcggag aggatcagcg agcggtgcag ctcagcgtca gcgagctagg
     2041 cacgtcgccg agccgatgaa gccgaacgtg gcggactgtc ccgcacccag ggggatcctc
     2101 tacagtcgac ctgcag
//