From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Saccharomyces cerevisiae transcription factor IIA (TFIIA) large
Date: 8 Apr 92 10:01:58 GMT
Distribution: bionet
Checksum: 31960     6

LOCUS       YSCTFIIAA    1856 bp ds-DNA             PLN       08-APR-1992
DEFINITION  Saccharomyces cerevisiae transcription factor IIA (TFIIA) large
            subunit (TOA1) gene, complete cds.
ACCESSION   M85248
KEYWORDS    TFIIA; transcription factor IIA.
SOURCE      Saccharomyces cerevisiae DNA.
  ORGANISM  Saccharomyces cerevisiae
            Eukaryota; Fungi; Eumycota; Hemiascomycetes; Endomycetales;
            Saccharomycetaceae.
REFERENCE   1  (bases 1 to 1856)
  AUTHORS   Ranish,J.A., Lane,W. and Hahn,S.M.
  TITLE     Isolation of two genes that encode subunits of the yeast
            transcription factor IIA
  JOURNAL   Science (1992) In press
  STANDARD  simple staff_entry
FEATURES             Location/Qualifiers
     5'UTR           1..390
                     /evidence=EXPERIMENTAL
     mRNA            315..1251
                     /note="alternate start"
                     /gene="TOA1"
                     /evidence=EXPERIMENTAL
                     /partial
     mRNA            320..1251
                     /note="alternate start"
                     /gene="TOA1"
                     /evidence=EXPERIMENTAL
                     /partial
     mRNA            334..1251
                     /note="alternate start"
                     /gene="TOA1"
                     /evidence=EXPERIMENTAL
                     /partial
     mRNA            338..1251
                     /note="alternate start"
                     /gene="TOA1"
                     /evidence=EXPERIMENTAL
                     /partial
     mRNA            345..1251
                     /note="alternate start"
                     /gene="TOA1"
                     /evidence=EXPERIMENTAL
                     /partial
     mRNA            357..1251
                     /note="alternate start"
                     /gene="TOA1"
                     /evidence=EXPERIMENTAL
                     /partial
     mRNA            375..1251
                     /note="alternate start"
                     /gene="TOA1"
                     /evidence=EXPERIMENTAL
                     /partial
     CDS             391..1251
                     /note="large subunit"
                     /product="transcription factor IIA"
                     /gene="TOA1"
                     /evidence=EXPERIMENTAL
                     /codon_start=1
                     /translation="MSNAEASRVYEIIVESVVNEVREDFENAGIDEQTLQDLKNIWQK
                     KLTETKVTTFSWDNQFNEGNINGVQNDLNFNLATPGVNSSEFNIKEENTGNEGLILPN
                     INSNNNIPHSGETNINTNTVEATNNSGATLNTNTSGNTNADVTSQPKIEVKPEIELTI
                     NNANITTVENIDDESEKKDDEEKEEDVEKTRKEKEQIEQVKLQAKKEKRSALLDTDEV
                     GSELDDSDDDYLISEGEEDGPDENLMLCLYDKVTRTKARWKCSLKDGVVTINRNDYTF
                     QKAQVEAEWV"
     3'UTR           1252..1856
                     /evidence=EXPERIMENTAL
BASE COUNT      680 a    298 c    375 g    503 t
ORIGIN
        1 gaattctgct cgcgttgctg agcttgccct aaaataagcc taactggagt ggtaggaact
       61 tcgttcatga attacgatat gtgctcaaaa ccttttttaa ctgggtgccc cttgacacca
      121 atgatgctct ttttagttag tgtccttcaa atgaaccatt aaagaggaac tcatcgtaca
      181 ctctgaatta taaataaata aataaagaat ataaattaac gcgaaaaaaa gcttcgtcaa
      241 gtatgatgtg agacagttga agaaaagatg aagaagccaa ataacaaggt ggataaagcc
      301 atacggcata ttatcggggg caagctcaaa gaagaaaagc gatagtatca cacaaatcaa
      361 ggccatacac aagggacatc gttggatatc atgtcgaatg cagaggccag cagagtgtac
      421 gagattatcg tagagtctgt ggtaaatgaa gtaagagagg actttgaaaa tgcgggtatc
      481 gatgaacaaa ctttacaaga cctaaaaaat atttggcaaa aaaagctcac agagacgaag
      541 gtaactactt tttcatggga caatcagttc aatgaaggaa atatcaatgg tgtacagaac
      601 gacttaaatt tcaatcttgc tacccccggc gtaaattcta gcgagttcaa tattaaggag
      661 gagaataccg gtaatgaagg tctaattttg ccaaatatca attccaacaa caacataccg
      721 cactctggag agaccaatat taatacgaat acggtagaag caactaataa ttcaggtgct
      781 accttgaaca caaatactag tggtaataca aatgcggacg taactagcca gcctaaaatt
      841 gaagtaaaac cggaaataga acttactatt aataatgcca atataactac cgtcgaaaac
      901 atagatgatg aaagtgagaa gaaagacgac gaggaaaagg aggaggacgt ggaaaaaacc
      961 cgcaaggaaa aggagcagat agaacaagta aaattacaag cgaagaagga aaaaagaagt
     1021 gcattattag atacggatga ggtcggttca gaactagatg attccgacga tgactatcta
     1081 atttcggagg gtgaagagga tgggccagat gagaacttaa tgctgtgctt atatgataaa
     1141 gtcacaagaa caaaggcgag atggaaatgt agtctgaaag atggtgtggt gaccatcaat
     1201 agaaatgact acacattcca aaaagctcaa gtggaagcgg agtgggtata actaaatctt
     1261 tagacgtaaa gaagaaaaaa tgtatatata caagtactat aatatcaaac taaaaaaacg
     1321 aaataaagag ggatgcaacg aacttggtca tctgttgtca aacagcgctt gtatgttcat
     1381 tagccgaagt gactttggct cggttccata gcttaaggag ttcagtaaca ccggaaccta
     1441 ctgaaattat ttcgtaagct agctctgagt tatatttgtc ccttttacct ttaatattaa
     1501 ttagattaga caataagttt agtattatct tataaaaatc caatgtaata ttatttttcc
     1561 gtaactccaa attgactttg tcattggtag gtagtttgta ctttaactcg tattccttta
     1621 ttgcattacc actggtatta tagatactta aataaatttc atctcttatt agctgtcgcc
     1681 attgttcaat gtttttattg atagcaagaa tatttaaaac ttcccataag atatagtgct
     1741 gttctttgac gaatgtcatt agtctattag tatttgcatg tgatgtgttc tttttaccaa
     1801 acattgatgt agcttatgga acagtatcaa ctcatctgtc aaattataaa aagctt
//