From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Cryptococcus albidus xylanase gene.
Date: 12 Aug 92 10:00:34 GMT
Distribution: bionet
Checksum: 25886     7

LOCUS       CPCXYLG      2984 bp ds-DNA             PLN       12-AUG-1992
DEFINITION  Cryptococcus albidus xylanase gene.
ACCESSION   X12596
KEYWORDS    xylanase.
SOURCE      Cryptococcus albidus.
  ORGANISM  Cryptococcus albidus
            Eukaryota; Fungi; Eumycota; Basidiomycotina; Filobasidiales.
REFERENCE   1  (sites)
  AUTHORS   Boucher,F., Morosoli,R. and Durand,S.
  JOURNAL   Nucleic Acids Res. 16, 9874-9874 (1988)
  STANDARD  full staff_review
REFERENCE   2  (bases 1 to 2984)
  AUTHORS   Morosoli,R., Durand,S. and Moreau,A.
  TITLE     Cloning and expression in Escherichia coli of a xylanase-encoding
            gene from the yeast Crytococcus albidus
  JOURNAL   Gene 117, 117-150 (1992)
  STANDARD  simple automatic
COMMENT
            [Unpublished (1988) see COMMENT for author address]  enum. 1 to
            2984.
            [1]  sites; for [Unpublished (1988) see COMMENT for author
            address].
            *source: clone=pIAF110; strain=CCY 17-4-4;
               EMBL features not translated to GenBank features:
               key        from     to       description
               PRM         614    620       put. TATA-box
               PRM         626    634       put. CAAT-box
               TRANSCR     670   2220       approx. primary transcript
               MSG         670   1016       exon 1
               IVS        1017   1070       intron I
               MSG        1071   1136       exon 2
               IVS        1137   1514       intron II
               MSG        1515   1879       exon 3
               IVS        1880   1930       intron III
               MSG        1931   2219       exon 4
            [Unpublished (1988) see COMMENT for author address]  Author address
            Morosoli R., Centre de Recherche en
            Microbiologie Applique, Institut Armand Frappier, 531 Boulevard des
            Prairies, Laval des Rapides, Ville de Laval, Quebec, Canada H7V B7.
            Submitted (08-AUG-1988) on tape to the EMBL data library.
FEATURES             Location/Qualifiers
     exon            <741..1016
                     /note="xylanase"
     CDS             join(741..1016,1071..1136,1515..1879,1931..2222)
                     /note="xylanase"
                     /codon_start=1
                     /translation="MLSSTTLLAILSALALTSVQAAPADKNSLDYLANKAGKRYLGTA
                     VQSPQLVPGSQYVQILESQFDAITPENEMKWEVVEPTEGNFDFTGTDKIVAEAKKTGS
                     LLRGHNICWDSQLRYAHEVAPKMKLCINDYNIETVNAKSQAMAKVAAGLLAKGAPLHC
                     IGMFKNAKRRSSGLLIRTASSGLESHFIGGSTPKDIPAAMNLFSDQGLEVPMTELDVR
                     IPVNGNDMPANATVAKEQVDDYYTSVSACLGNDLCPGVSIWQFADPTSWIPGVFKGKL
                     IAVSCTFSGCLLQYCVGYGAALLYDAQYQPKSTYYVVQQALKDGKNSGSKFHGIKL"
     exon            1071..1136
     exon            1515..1879
     exon            1931..>2222
                     /note="xylanase"
BASE COUNT      733 a    847 c    716 g    688 t
ORIGIN
        1 gcatgcgggg ccagtttggc aggtggaggt tccatgggca tagcgatcca tatggtagct
       61 catatggtga acattccgtt tcgcccgatt tcttggtgcc tggaccgcga cacctcttcc
      121 ctgttcctgg gcctacctct gtcgctcgga cgaaatttcc cacccgacaa gtggggcaac
      181 gagccgggcc agcgattccc ctaatgacga gcaattatgc ggacaagcta gaagaagagt
      241 tgctttgtcg gaagccgccg atgagggtgc tgttgggaaa tatgggaagc attcacgatc
      301 gttaacctag ctgttcatcc gtcctcattc tggcctccta caggtattca ggcatctatt
      361 cttgctgctc accagctcat ctgctgttcc tgcactcccg gaatagggtc gctcgctaca
      421 tacggcgttg atggcaaagc aagtctagat gcaagatggc ggactgcaag tttcccaagg
      481 tacgaggaat ccaggagccc ctctggtctc caagtgacca gcgaatgccg accgacaacg
      541 atcgtgctcg ggttcgaaag ccggcagggc ctcaacttca cgacgtgcca agaacgaaaa
      601 gaggtagaag caatatataa tgcttcacca atccaccatt ccagttcttg cactttccct
      661 tacaaccaag cttcctcgct agactttcgc ctcgtcactg ctcttgccaa accaaaccaa
      721 accgttctta caactccaag atgctctctt caaccacctt gctcgccatt ctctcagcgt
      781 tggctctgac cagcgtccaa gcggcccccg ccgataagaa ctctctcgac tacttggcga
      841 acaaggccgg caaacgatac ctcggaaccg ccgttcagtc gcctcagctt gtccccggct
      901 cgcaatatgt ccagatcctc gaaagccagt tcgacgccat tactcctgaa aacgagatga
      961 agtgggaagt cgttgagccg accgagggta actttgattt caccggtacc gacaaggtga
     1021 gcctggagtc ataagtgaca aagcagtcag acttacccta cttgctccag atcgtcgccg
     1081 aagccaagaa gactggttct ctgctccgag gccacaacat ctgctgggac tctcaggtaa
     1141 gtggtccaag aaagtaaagc gatacgacga ctgatcatgg gctctagacc cctgcatacg
     1201 tgaccagcat tacggacccg acaaagctca agaaggttct caagaaccac atcgacaacg
     1261 tgatcggtcg atacaaggac gatctcgcat actttgacat tgtcaacgag cgtgagtagc
     1321 ctcggccgcg accgaatcgc ttcgaatact taacgatggt tttcctttct tttcctccag
     1381 ctcttaacga gaacggtact tacaaatcca acgtttggta caatgtcctc ggtgaatcct
     1441 acattgagac tgcagtaagc cacgcgattc attctcgaaa tcctcaacgt tgatcagtca
     1501 cctttttgct atagttgcgg tatgctcacg aagttgctcc caagatgaag ctctgcatca
     1561 acgactacaa catcgagacc gtcaacgcga agagtcaggc gatggccaag gttgctgctg
     1621 gtttgcttgc caagggagct cctctccact gcatcggtat gttcaagaat gcgaagagac
     1681 gatcgtccgg actgctgatc cgaaccgcat cttcaggtct cgaatcacac ttcatcggtg
     1741 gttccactcc caaggacatc cccgccgcca tgaacttgtt cagtgaccag ggtctcgaag
     1801 tccccatgac tgaactcgat gttcgaattc cggtcaacgg aaatgatatg ccggccaacg
     1861 ccaccgtagc caaggagcag taagtgcttc gcaatcacga aagatcagag gctaacacgt
     1921 tcgagtgcag agtcgatgac tactacacca gtgtttccgc ttgtctcgga aacgacctct
     1981 gcccgggtgt cagcatctgg caattcgctg accccacttc atggatcccc ggagttttca
     2041 agggtaagtt gattgcggtc tcctgcacat tctcaggatg cttattgcaa tactgcgtag
     2101 gttacggagc ggcactcttg tacgatgctc aataccagcc caagtcgacc tactacgttg
     2161 tgcaacaagc tctcaaggac ggcaagaaca gtggcagcaa gttccacggc atcaagcttt
     2221 aaacttctgt cgcgtcgaca ccgaggaacc aggaagccaa agaagatggc aagaacgcta
     2281 tccctcatgt atccccagga aattcaagca ggaaagtatg cattgtagca aagtcgtttt
     2341 caaaagaaag tcacgtagta gtcatgatca gagtatgcaa ttatacaaga gcgtctcatg
     2401 caaacctggg acaggcgtcg cgatctcgcg atactcctct gccgcgcaca agtcacaaca
     2461 attgacatag caggcagcgc ctcatcgacg taactccttt cctttagcaa cgagggacta
     2521 accagtcgac ttatcctagc ctttcatctt gtcctttcag aaattattgt acggctttca
     2581 ggtcatgcca tgccgcgctg tgaaattcag cccgtgatga ccatacacgc cctatgagca
     2641 aagctactct tcgaatacga gatagagatc actcggctac gacatgccgt gatgcgcgcg
     2701 cggtgcgaaa agctttcgct cagtgtattc cattgccttc atacatacaa cttcggctat
     2761 tgcgcgcctt acatctgacc gagcgtaagc ccagcgggga agccagcaaa gtcgacctgt
     2821 tcctgcacgg tctccccgct ttccgactcg acgtaagaga tccgtagtcg caatcggact
     2881 tgcgctcctg gtggagcaat gacccgcatt tgttgcgttt ctgttgcacc gggcgcaaca
     2941 tcgccgttgg acatggcgag catctgtagt tgttgtgtct gcag
//