From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: M.musculus gene for anti-CEA mAb T84.66 kappa light chain V-region
Date: 3 Jan 93 22:07:30 GMT
Distribution: bionet
Checksum: 30917 4

LOCUS       MUSCEALCH    1014 bp ds-DNA             ROD       21-DEC-1992
DEFINITION  M.musculus gene for anti-CEA mAb T84.66 kappa light chain V-region
ACCESSION   X52768
KEYWORDS    CEA; light chain; tumor-associated antigen.
SOURCE      Mus musculus DNA.
  ORGANISM  Mus musculus
            Eukaryota; Animalia; Chorqata; Vertebrata; Mammalia; Theria;
            Eutheria; Rodentia; Myomorpha; Muridae; Murinae.
REFERENCE   1  (bases 1 to 1014)
  AUTHORS   Neumaier,M., Shively,L., Chen,F.S., Gaida,F.J., Ilgen,C.,
            Paxton,R.J., Shively,J.E. and Riggs,A.D.
  TITLE     Cloning of the genes for T86.66, an antibody that has a high
            specificity and affinity for carcinoembryonic antigen, and
            expression of chimeric human/mouse T84.66 genes in myeloma and
            Chinese hamster ovary cells
  JOURNAL   Cancer Res. 50, 2128-2134 (1990)
  STANDARD  full automatic
COMMENT     *source: clone_library=partial MboI genomic;
               From EMBL    entry MMCEALCH;  dated 03-DEC-1992.
FEATURES             Location/Qualifiers
     promoter        181..190
     exon            273..321
                     /number=1
     intron          322..560
                     /number=1
     exon            561..905
                     /number=2
     CDS             join(273..321,561..905)
                     /partial
                     /partial
                     /note="light chain"
                     /product="anti-CEA mAb T84.66"
                     /codon_start=1
                     /translation="METDTLLLWVLLLWVPGSTGDIVLTQSPASLAVSLGQRATMSCR
                     AGESVDIFGVGFLHWYQQKPGQPPKLLIYRASNLESGIPVRFSGTGSRTDFTLIIDPV
                     EADDVATYYCQQTNEDPYTFGGGTKLEIK"
BASE COUNT      260 a    221 c    235 g    298 t
ORIGIN      
        1 gatcctggtc tacagtgtga ggtactggac aaccaagaat agagaaacct gtctcaaaaa
       61 aatgtattta gaagggtccc ttaagacaca tgtgatgttc tagtaaattt tctgctaaca
      121 ccaacttcct ctgggtgaaa caggggcagg tgcacatgga gaaacagtac atactctgct
      181 gatttgcata tgaaataatt tataacagcc caggcttctt taaggcagct gccaggagcc
      241 taataaagca tcctctcttc cagctctcag agatggagac agacacactc ctgctatggg
      301 tgctgctgct ctgggttcca ggtgagggtg ccgagaagtg ttgagagaaa accctctgtg
      361 gacatcatga ttttgcttgc ctgtgggttc ataaacataa taattaacgc acttgtaatt
      421 ggttttgttt cctgagaccc cttcatttcc tggtgtctca taggatgtcc gcaatattct
      481 tgatattttt aaatgaaaag gtccctgctg ggaaggcttt tacacataaa taacagtagt
      541 ctgtgtgttt atcattccag gttccacagg tgacattgtg ctgacccaat ctccagcttc
      601 tttggctgtg tctcttgggc agagggccac catgtcctgc agagccggtg aaagtgttga
      661 tatttttggc gttgggtttt tgcactggta ccagcagaaa ccaggacagc cacccaaact
      721 cctcatctat cgtgcatcca acctagaatc tgggatccct gtcaggttca gtggcactgg
      781 gtctaggaca gacttcaccc tcatcattga tcctgtggag gctgatgatg ttgccaccta
      841 ttactgtcag caaactaatg aggatccgta cacgttcgga ggggggacca agctggaaat
      901 aaaacgtaag tagtcttctc aactcttgtt cactaagtct aaccttgtta agtgttcttt
      961 gttgtgtgtt tttcttaagg agatttcagg gatttagcaa attccattct caga
//