From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: L. lactis genes for DNA primase and RNA polymerase sigma factor.
Date: 6 Jan 93 06:49:50 GMT
Distribution: bionet
Checksum: 65160 6

LOCUS       LACDERD      2670 bp ds-DNA             BCT       06-JAN-1993
DEFINITION  L. lactis genes for DNA primase and RNA polymerase sigma factor.
ACCESSION   D10168
KEYWORDS    DNA primase; RNA polymerase sigma factor.
SOURCE      Lactococcus lactis (strain ATCC 19435) genomic DNA, clone pUC118.
  ORGANISM  Lactococcus lactis
            Prokaryotae; Bacteria; Firmicutes; Regular asporogenous rods;
            Lactobacillaceae.
REFERENCE   1  (bases 1 to 2670)
  AUTHORS   Araya,T.
  JOURNAL   Unpublished (2048)
  STANDARD  full automatic
COMMENT     These data kindly submitted in computer readable form by: Tomoko
            Araya Nutritional Science Laboratory Morinaga Milk Industry Co.,
            Ltd. 5-1-83 Higashihara Zama, Kanagawa 228 Japan Phone:
            0462-52-3047 Fax:    0462-52-3055.
FEATURES             Location/Qualifiers
     misc_signal     1293..1296
                     /note="SD sequence"
     terminator      2328..2361
                     /note="rho factor dependent terminator"
     CDS             <1..918
                     /gene="dnaE"
                     /product="DNA primase"
                     /codon_start=1
                     /translation="DPDEYSKNYGLKGLSALMETGRIQPIEFLIDYLRPENLANLQTQ
                     LDFIEQISPMIAKLPSITAQDAYIRKLVEILPDFEYNQVEQAVNLRRENMKITDHPVS
                     NLDASSLTESFTDENDYSSLESVMPVDFEETYYENNVKTQQTYRRSESAQVVQPSVQV
                     PKLSRSEKAEEMLLHRMIYHSSVLKKFSQDENFRFVHKRYQDLFDKILLEAMVYEQID
                     ESHLASELSDEERSLFYQIISLDLPDTASSQEINDLVSIFSNEMEQIKFEELIQQLAT
                     AEKAGNKERVLELTLQIINQNEKIMIIFY"
     CDS             1302..2324
                     /gene="rpoD"
                     /product="RNA polymerase sigma factor"
                     /codon_start=1
                     /translation="MDELSVKFGIVDDALEDLFKQIQDAGISIVDKDGNPSPLALVTD
                     EIEKEEVSDTAMDEIVTNVRIDDPVRMYLKEIGRYPLISLDEETKLAEAIIAGGEGAE
                     FAKQMLAEANLRLVVSIAKRYSGRGMQFLDLIQEGNMGLMKAVDKFDHTKGFKFSTYA
                     TWWIRQAITRAIADQARTIRIPVHMVETINKLIRVQRNLLQELGRDPSPEEIGKELHM
                     APDKVREVLKIAQEPVSLETPIGEEDDSHLGDFIEDDVIESPVDYTNRILLREQLDEV
                     MDTLTDREENVLRMRFGLDDGRMHTLEDVGKQFKVTRERIRQIEAKAIKKLRHPRRSK
                     PLRDFM"
BASE COUNT      908 a    402 c    530 g    830 t
ORIGIN      
        1 gatccagatg agtattctaa aaattatggt ttgaagggat tatctgcctt aatggaaacg
       61 gggcgtattc aacccattga atttttaatt gattatttga gacctgaaaa ccttgctaat
      121 ttacaaacgc aacttgattt tattgaacag atttcaccaa tgattgcaaa gcttccatcg
      181 attacggctc aggatgccta tataagaaaa ttagttgaga ttttgcctga tttcgagtat
      241 aatcaagtgg agcaggctgt aaatttacgg cgtgaaaata tgaaaattac tgaccatcct
      301 gtcagtaatt tagatgcttc aagtttgacc gaaagtttta ctgacgaaaa tgattattct
      361 agtcttgaat cggtcatgcc tgtagatttt gaggagactt attatgagaa taatgttaaa
      421 actcaacaaa cttacagaag aagtgaatcg gctcaagttg ttcaaccatc tgttcaggtt
      481 cctaagttat cacgttcgga aaaagccgaa gaaatgctac tccatcgaat gatttatcat
      541 tcatcagttt tgaaaaaatt ttctcaagat gaaaatttta gattcgtcca taaacgttat
      601 caagatttat ttgataaaat cttgcttgaa gcaatggttt atgaacaaat tgatgaatca
      661 cacttagcaa gtgagctgtc agacgaagaa cgtagtctgt tttaccagat tataagttta
      721 gatttgcctg atacagcatc aagtcaggaa atcaatgact tagtttccat tttttctaat
      781 gaaatggaac aaataaaatt tgaagaactc attcaacagt tggcaactgc tgagaaagca
      841 ggaaacaaag aacgtgtctt agagttaacc cttcagatta ttaaccaaaa tgaaaaaatt
      901 atgataattt tttactgaca attggctagt ctatttaatg acagttttgt cagtagtttt
      961 acccaaagag gaggagcgaa ttggcagcaa ataaaaaaag aataagtcaa atttcaaaag
     1021 aaactggcgt tcaaaaccgt gttttggttg agaaggctca agaaattggc ttacttgtaa
     1081 aatcacattc aagttcagtt ggacctggtg aagaacgtcg tcttcttgaa gcattaaatg
     1141 ttaaacctgt tgttgttgaa aagatggata gtcctaagac attagcaaga gttgaagtca
     1201 aagaaaatgg tgatactttt aacgtaaaag cttatgaaaa agcagttaaa agctatatta
     1261 ccaaacgtaa acctttgggt gaagcgcttg atgaagaaat catggatgaa ctttctgtta
     1321 aatttgggat tgttgatgat gcgcttgaag acttgtttaa acaaattcaa gatgcaggaa
     1381 tttcaattgt ggataaagat ggaaatccaa gtcctttggc tcttgtcact gatgaaattg
     1441 aaaaagaaga agtttcagat actgcaatgg atgaaattgt tacgaatgtt cgtatcgatg
     1501 atcctgttcg catgtatctt aaagaaatcg gtcgttatcc attgatttct cttgatgaag
     1561 aaacaaaact tgctgaagca attattgctg gtggtgaagg ggcagaattt gcgaaacaaa
     1621 tgcttgccga agccaacttg cgtttggttg tttcaattgc aaaacgttat tctggacgtg
     1681 gaatgcaatt tttggacttg attcaagaag gaaatatggg cttgatgaag gctgtggata
     1741 aatttgacca tacaaaagga ttcaagttct cgacttatgc cacatggtgg attcgccaag
     1801 caattacacg tgcgattgct gaccaagccc gcacgattcg tattccagtg catatggttg
     1861 aaacaatcaa taaattgatt cgtgttcaac gtaatctttt gcaagaactc ggacgcgatc
     1921 cttcaccaga agaaattggt aaagagttgc acatggcgcc agataaagtt cgtgaagttc
     1981 tgaaaattgc tcaagaacca gtctcactcg aaacacctat cggtgaagaa gatgattcac
     2041 atcttggtga cttcattgaa gatgatgtaa tcgaatcacc tgttgattat acaaaccgta
     2101 ttcttttacg tgaacagtta gatgaagtca tggatacttt gactgaccgt gaagaaaatg
     2161 ttttgagaat gcgttttggt cttgatgatg gacgcatgca tactttggaa gatgttggta
     2221 aacaattcaa agtgacacgt gaacgtattc gtcagattga agcaaaagca atcaaaaaac
     2281 ttcgtcaccc acgtcgttct aaaccgttgc gtgactttat gtaatttaaa aacttactga
     2341 tgaaactgtc agtaagtttt tttatatttt tttagagata gtatgtatta tgattcagtt
     2401 tgtattatat aaaattattt tttattcaga ggagttttat gaaccattta actagtgtcg
     2461 aaatttatat ttggaattat ttagaagaaa ataaagcaaa aataattcaa atgacggttg
     2521 ctcaaattgc tgaatctgcc catgtcagtc cttcatcaat tattcgaact ttgaaaaaga
     2581 aaaatttttc tggtttttct gaatacaaaa atatggaaaa acaaaaatta aatagacatg
     2641 attggattta tgggctgaca aaagatcgaa
//