HDTenEightyP/NetNews huggingface dataset:
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Lentinus sulcatus ribosomal RNA (33LR0R) large subunit, partial.
Date: 19 Aug 92 10:01:52 GMT
Distribution: bionet
Checksum: 47565     2

LOCUS       LENRRLSAU     225 bp ss-rRNA            RNA       19-AUG-1992
DEFINITION  Lentinus sulcatus ribosomal RNA (33LR0R) large subunit, partial.
ACCESSION   M98655
KEYWORDS    large subunit ribosomal RNA.
SOURCE      Lentinus sulcatus (library: Duke culture collection 797) cDNA to
            rRNA.
  ORGANISM  Lentinus sulcatus
            Eukaryota; Fungi; Eumycota; Basidiomycotina; Hymenomycetes;
            Agaricales; Tricholomataceae.
REFERENCE   1  (bases 1 to 225)
  AUTHORS   Hibbett,D.S. and Vilgalys,R.
  TITLE     Phylogenetic relationships of the basidiomycete genus Lentinus
            inferred from molecular and morphological characters
  JOURNAL   Unpublished (1992)
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     rRNA            <1..>225
                     /note="Aligns to region between positions 70 to 306
                     inSaccharomyces cerevisiae large subunit nuclear rRNA."
                     /product="large subunit ribosomal RNA"
                     /citation=[1]
BASE COUNT       55 a     45 c     68 g     57 t
ORIGIN
        1 ccctaggaac ggcgagtgaa gcgggaaaag ctcaaattta aaatctggcg gtcttcggcc
       61 gtccgagttg tattctggag aagtgctttc tggaccgtgt ataagtctct tggaacagag
      121 cgtcacagag ggtgagaatc ccgtctttga cacggactac cagtgctttg tgatgcgctc
      181 tcaaagagtc gagttgtttg ggaatgcagc tcaaaatggg tggta
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Simian immunodeficiency virus long terminal repeat sequence.
Date: 19 Aug 92 10:03:50 GMT
Distribution: bionet
Checksum: 03714     2

LOCUS       SIVTYOAB      703 bp ds-DNA             VRL       19-AUG-1992
DEFINITION  Simian immunodeficiency virus long terminal repeat sequence.
ACCESSION   M63387
KEYWORDS    long terminal repeat.
SOURCE      Simian immunodeficiency virus (isolate TYO-5) proviral DNA, clone
            pSA5-CAT.
  ORGANISM  Simian immunodeficiency virus
            Viridae; ss-RNA enveloped viruses; Positive strand RNA virus;
            Retroviridae; Lentivirinae.
REFERENCE   1  (bases 1 to 703)
  AUTHORS   Sakuragi,J.-I., Hayami,M., Ishimoto,A., Kiyomasu,T., Akari,H.,
            Kawamura,M., Sakai,H., Shibata,R., Fukasawa,M. and Adachi,A.
  TITLE     Functional analysis of long terminal repeats derived from four
            strains of simian immunodeficiency virus SIV-AGM in relation to
            other primate lentiviruses
  JOURNAL   Virology 185, 455-459 (1991)
  STANDARD  simple staff_entry
FEATURES             Location/Qualifiers
     LTR             1..478
                     /note="U3 region"
     LTR             604..703
                     /note="U5 region"
BASE COUNT      175 a    148 c    197 g    183 t
ORIGIN
        1 actggatggg atttattact ctgatagaag ggctaaaata ctcaatctgt atgccttaaa
       61 tgagtgggga atcatagatg actggcaggc atacactcca ggaccgggaa tcaggtaccc
      121 aaagtgtttt gggttctgtt tcaagttggt accagttgtc ctccatgagg aggcagaaac
      181 atgtgaaagg cattgcttgg tccacccagc tcaaatgagg gaagatcctg atgatccaga
      241 acataatgag gtgctggtat ggaagtttga tcccatgcta gcctgtaagg cctcgccagg
      301 ggtattcaca gacatgcata atgtgaagaa gtaggatgtg ggtttgcggt taggcgcatc
      361 cgggagctgt gtaccaacca tcatagcaac gggtcgggac tttccaggct gggaggtcct
      421 aggggagtgg ttttgccctc agaattgtat aaaagcagat gctcttggct tgtaacttag
      481 gtctcttact aggggaccag cttgagcctg ggtgttcgct ggtgaaccta acttgacttg
      541 gccagtcaag ggtaagagcc tccacggctt gaatgctatg cttcaataaa ttttgcctgc
      601 tttagagcac ttcgagtcgt gtggtcccat taccgtctcc gtgcagaagg gaatccttca
      661 tactggggtt tctctgaccc aggagagaaa aacctccagc agt
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human chromosome 4 sequence-tagged site STS4-430
Date: 21 Aug 92 10:10:17 GMT
Distribution: bionet
Checksum: 17013     3

LOCUS       HUM4STS430    450 bp ds-DNA             PRI       21-AUG-1992
DEFINITION  Human chromosome 4 sequence-tagged site STS4-430
ACCESSION   L00856
KEYWORDS    STS; human chromosome 4; sequence tagged site.
SOURCE      Homo sapiens, clone p4-398 from a chromosome 4 plasmid library;
            vector pBluescript II KS + (Stratagene)
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 450)
  AUTHORS   Goold,R.D., diSibio,G., Dugaiczyk,A., Smith,K.A., Xu,H.,
            Magrane,G., Lang,D.B., Cox,D.R., Masters,S.B. and Myers,R.M.
  TITLE     Sequence-Tagged Sites for Human Chromosome 4
  JOURNAL   Unpublished (1992) see COMMENT for author address
  STANDARD  full automatic
COMMENT     PCR components: 25 ng of human genomic DNA, 10 pmol of each
            oligonucleotide, 200 micro-M dNTPs, 0.25 U Taq  polymerase (Cetus)
            in 10 micro-l of 50 mM KCl-20 mM  Tris-HCl, pH 8.3 (at room temp),
            2.5 mM MgCl-2.  Initial denaturation at 94degC for 1.5 min, then 30
            cycles of  94degC for 15 sec, 62degC for 23 sec, and 72degC for 30
            sec, followed by a final extension at 72degC for 3.5 min, using a
            Perkin-Elmer 9600 thermocycler. PCR-amplified product size 150 bp.
            STS sequence contains a simple sequence tandem repeat (CA).
            Sequence submitted by:
            Human Genome Mapping Center
            Box 0925
            University of California San Francisco
            San Francisco, CA 94143-0925  USA
            Phone: (415) 502-1612   Fax: (415) 476-8391
            e-mail: hgmcprobes@cgl.ucsf.edu
FEATURES             Location/Qualifiers
     misc_feature    89..112
                     /label=Primer1
     misc_feature    complement(214..238)
                     /label=Primer2
BASE COUNT      153 a     70 c     85 g    129 t     13 others
ORIGIN
        1 gatctatagg tgtatcctgt tttagtttaa ttgnattgaa aaatacatac caagtttttg
       61 ncatgttcaa gacacagttc tggggaccac agtaaagagg aggatggata agacttccta
      121 tatgagggaa atatnggnaa aataantaca caaataactc tggtgttagt ttcatgtgaa
      181 aatattttgg cacaacacac acacacacac acacacagga ttcaagaaga aaggacttcc
      241 atacattnng gggggataat acagattaca tttaccatat tttgnacaat cattgtgtgc
      301 taaacactta aaagggtatc tctcatttac ttntcacaac atttttatga gataggtaca
      361 agcatgtcag tatttcatna aatgaggaaa ctgaatcttg gcgtaataaa gtantngctt
      421 acggtcacct gtgtngtaat gaggtgaacc
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human chromosome 4 sequence-tagged site STS4-357
Date: 21 Aug 92 10:09:21 GMT
Distribution: bionet
Checksum: 45494     3

LOCUS       HUM4STS357    429 bp ds-DNA             PRI       21-AUG-1992
DEFINITION  Human chromosome 4 sequence-tagged site STS4-357
ACCESSION   L00814
KEYWORDS    STS; human chromosome 4; sequence tagged site.
SOURCE      Homo sapiens, clone p4-313 from a chromosome 4 plasmid library;
            vector pBluescript II KS + (Stratagene)
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 429)
  AUTHORS   Goold,R.D., diSibio,G., Dugaiczyk,A., Smith,K.A., Xu,H.,
            Magrane,G., Lang,D.B., Cox,D.R., Masters,S.B. and Myers,R.M.
  TITLE     Sequence-Tagged Sites for Human Chromosome 4
  JOURNAL   Unpublished (1992) see COMMENT for author address
  STANDARD  full automatic
COMMENT     PCR components: 25 ng of human genomic DNA, 10 pmol of each
            oligonucleotide, 200 micro-M dNTPs, 0.25 U Taq  polymerase (Cetus)
            in 10 micro-l of 50 mM KCl-20 mM  Tris-HCl, pH 8.3 (at room temp),
            2.5 mM MgCl-2.  Initial denaturation at 94degC for 1.5 min, then 30
            cycles of  94degC for 15 sec, 62degC for 23 sec, and 72degC for 30
            sec, followed by a final extension at 72degC for 3.5 min, using a
            Perkin-Elmer 9600 thermocycler. PCR-amplified product size 208 bp.
            STS sequence contains a simple sequence tandem repeat (CA).
            Sequence submitted by:
            Human Genome Mapping Center
            Box 0925
            University of California San Francisco
            San Francisco, CA 94143-0925  USA
            Phone: (415) 502-1612   Fax: (415) 476-8391
            e-mail: hgmcprobes@cgl.ucsf.edu
FEATURES             Location/Qualifiers
     misc_feature    21..46
                     /label=Primer1
     misc_feature    complement(204..228)
                     /label=Primer2
BASE COUNT      152 a     94 c     57 g    119 t      7 others
ORIGIN
        1 gatccttagc actaacagna agccacttta atcccaaatt ctacaaatag aagcacccga
       61 aaaccttacc aagtgcagaa cagcttattt aaacacacac acacacacac acacacacac
      121 acacacacac acacctggna gtaggctttg gntttcccag cacctttttt tttcttaatt
      181 tttaagactg caattctcac ctagatactg gtactaatgc agaataggta ttccataaac
      241 atntgntcaa acaacaaata cactaaggtg atttccacgt atattnngtg aatttgaaat
      301 tgatgcaatt acttaaatta aaaaatgggt cttcagttac aagcactcta caatctaatt
      361 acgtgaatac attgctaagc gctattttat tacctatgta gacagaagat taatgttctc
      421 agggaatga
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Glycine max copia-like retrotransposon reverse transcriptase gene,
Date: 14 Aug 92 10:01:24 GMT
Distribution: bionet
Checksum: 24684     2

LOCUS       SOYCOPIA      276 bp ds-DNA             PLN       14-AUG-1992
DEFINITION  Glycine max copia-like retrotransposon reverse transcriptase gene,
            partial cds.
ACCESSION   M94493
KEYWORDS    copia-like element; retrotransposon; reverse transcriptase.
SOURCE      Glycine max DNA.
  ORGANISM  Glycine max
            Eukaryota; Plantae; Embryobionta; Magnoliophyta; Magnoliopsida;
            Rosidae; Fabales; Fabaceae.
REFERENCE   1  (bases 1 to 276)
  AUTHORS   Voytas,D.F., Cummings,M.P., Konieczny,A., Ausubel,F.M. and
            Rodermel,S.R.
  TITLE     Copia-like retrotransposons are ubiquitous among plants
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 89, 7124-7128 (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     CDS             5..271
                     /note="PCR product"
                     /product="reverse transcriptase"
                     /codon_start=1
                     /translation="RTAFLHGRLEENILMKQPEGFEVQGKERYVSQLQRSLYGLKQSP
                     RQWYMSFDSFITNQGFKRSLYDCCVYHNKVEDGLMIYLLLYVDDM"
                     /partial
BASE COUNT       85 a     45 c     68 g     78 t
ORIGIN
        1 ctgcaggaca gcgtttttgc acggaagatt ggaagaaaat attctaatga aacagcctga
       61 agggtttgaa gttcaaggaa aagaaaggta tgtttctcaa ctgcagaggt ccttgtatgg
      121 tttgaaacaa tctccaaggc aatggtacat gagttttgac agctttatca ccaaccaagg
      181 attcaagaga agtctctatg actgctgtgt ttatcacaac aaggtggagg atggattaat
      241 gatctatctt ctgctgtacg tggatgatat ggatcc
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Calyptogena magnifica symbiont 16S ribosomal RNA (16S rRNA).
Date: 20 Aug 92 10:01:21 GMT
Distribution: bionet
Checksum: 54733     3

LOCUS       CPG16SRRNA   1409 bp ss-rRNA            RNA       20-AUG-1992
DEFINITION  Calyptogena magnifica symbiont 16S ribosomal RNA (16S rRNA).
ACCESSION   M99446
KEYWORDS    16S ribosomal RNA.
SOURCE      Calyptogena magnifica symbiont rRNA.
  ORGANISM  Calyptogena magnifica symbiont
            Prokaryotae; Proteobacteria gamma subdivision.
REFERENCE   1  (bases 1 to 1409)
  AUTHORS   Distel,D.L.
  TITLE     Sulfur-oxidizing bacterial endosymbionts: analysis of phylogeny and
            specificity by 16S rRNA sequences
  JOURNAL   J. Bacteriol. 170, 2506-2510 (1988)
  STANDARD  full staff_entry
FEATURES             Location/Qualifiers
     rRNA            1..1409
                     /product="16S ribosomal RNA"
                     /gene="16S rRNA"
BASE COUNT      383 a    291 c    416 g    318 t      1 others
ORIGIN
        1 cgatactagt ttactagtag gcgtcgagtg gcggacgggt gagcaacgcg taggaatctg
       61 cctgatagtg ggggatagcc cgaagaaatt cggattaata ccgcataatc tctacggagc
      121 aaaggaggcc tctatttata tgctttcgct atcagatgag cctgcgtaag attagctagt
      181 tggtgaggta aaagcttacc aaggcaacga tctttagctg gtctgagagg atgatcagcc
      241 acactggaac tgagacacgg tccagactcc tacgggaggc agcagtgggg aatattggac
      301 aatgggcgca agcctgatcc agcaataccg cgtgtgtgaa gaaggcctga gggttgtaaa
      361 gcactttcaa ttaggaagaa aaactaatgg ttaataccca ttagtcttga cgttactttt
      421 agaagaagca ccggctaact ccgtgccagc agccgcggta atacggaggg tgcaagcgtt
      481 aatcggaatt actgggcgta aagcgtgcgt aggtggtttg ttaagttaga tgtgaaagcc
      541 ccgggctnaa cctgggaact gcatttgaaa ctggcaaact agagtatagg aggggaaagt
      601 ggaatttctg gtgtagcggt gaaatgcgta gatatcagaa ggaacatcaa tggcgaaggc
      661 tgctttctgg acaaatactg acactgaggt acgaaagcgt gggtagcaaa caggattaga
      721 taccctggta gtccacgccg taaacgatga taactagccg ttggggggaa gtgcccttta
      781 gtggcgaagc taacgcgtta agttatccgc ctggggagta cgaccgcaag gttaaaactc
      841 aaaggaattg acggggaccc gcacaagcgg tggagcatgt ggtttaattc gatgcaacgc
      901 gaagaacctt acctggtctt gacatatcgc aaacttttca gagatggaga ggtgccttcg
      961 ggaatgcgaa tacaggtgct gcatggctgt cgtcagctcg tgtcgtgaga tgttgggtta
     1021 agtcccgtaa cgagcgcaac ccttatcctt atttgccagt acgtcatggt gggaactata
     1081 aggagactgc cggtgataaa ccggaggaag gcggggacga cgtcaagtca tcatggccct
     1141 tacgaccagg gctacacacg tgctacaatg ggaaggacaa agggtcgcta agccgtgagg
     1201 tagtgctaat ctcacaaatc ttttcgtagt ccggatcgga gtctgcaact cgactccgtg
     1261 aagtcggaat cgctagtaat cgtagatcag aacgctacgg tgaatacgtt cccgggtctt
     1321 gtacacaccg cccgtcatac catgggagtg ggttgcacca gaagtagcta gtctaactgt
     1381 ttacagagga cggttaccac ggtgtgatt
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Eubacterium sp. (Octopus Spring I25 locus) gene for 16S rRNA
Date: 22 Aug 92 10:00:27 GMT
Distribution: bionet
Checksum: 45271     2

LOCUS       EUB16SRRN2    278 bp ds-DNA             BCT       22-AUG-1992
DEFINITION  Eubacterium sp. (Octopus Spring I25 locus) gene for 16S rRNA
            (partial)
ACCESSION   X67085
KEYWORDS    16S rRNA; rRNA.
SOURCE      Eubacterium sp. DNA.
  ORGANISM  Eubacterium sp.
            Prokaryotae; Firmicutes; Irregular asporogenous rods.
REFERENCE   1  (bases 1 to 278)
  AUTHORS   Ward,D.M., Weller,R. and Bateson,M.M.
  TITLE     16S rRNA sequences reveal uncultured inhabitants of a well-studied
            thermal community
  JOURNAL   FEMS Microbiol. Rev. 75, 105-116 (1990)
  STANDARD  full automatic
COMMENT     *source: strain=OS-I-25; *source: isolate=Octopus Spring,
            Yellowstone National Park, USA;
            From EMBL    entry ES16SRRN2;  dated 08-JUL-1992.
BASE COUNT       66 a     60 c     89 g     52 t     11 others
ORIGIN
        1 gtaaagcgca cgtaggckkc tttcgtaagt ctggtgtgaa atctcacggc tcaaccgaga
       61 ggctgcacca gatactgcga agctygagna cgggagagga gatcggaatt cccggtgtag
      121 cggtgaaatg cattgatatc gggaggaaca ccagtggcga aggcggatct ctggcccgtn
      181 tacgctgagg tnnnaaagct gggggagcgn aaggattaga tacccctgta gtcccagccc
      241 taaacgatgg gcgctaggtg ttggggnntt aaccctca
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human patient CG clonal evolution of a follicular lymphoma.
Date: 13 Aug 92 10:00:37 GMT
Distribution: bionet
Checksum: 48758     2

LOCUS       HUMFOLLYM     291 bp ds-DNA             PRI       13-AUG-1992
DEFINITION  Human patient CG clonal evolution of a follicular lymphoma.
ACCESSION   M94768
KEYWORDS    .
SOURCE      Homo sapiens DNA.
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 291)
  AUTHORS   Bahler,D.W. and Levy,R.
  TITLE     Clonal evolution of a follicular lymphoma: Evidence for antigen
            selection
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 89, 6770-6774 (1992)
  STANDARD  full staff_entry
BASE COUNT       66 a     84 c     80 g     61 t
ORIGIN
        1 caggtgcaac tacaacagtg gggcgcagga ttgttgaagc cttcggagac cctgtccctc
       61 acctgcgctg tctatggtgg gtctttcaga aattacaact ggacttggat ccgccagccc
      121 cccgggaagg ggctggagtg gattggggga gtcaatcata ctggacgcac caactacaac
      181 ccgtccctca ggggtcgagt caccatatca gttgacacgt ccaagaacca gttctccctg
      241 acaatgacct ctgtgaccgc cgcggacacg gctctatatt actgtgcgag a
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Ossicaulis lignatilis ribosomal RNA (44LR3) large subunit, partial.
Date: 19 Aug 92 10:03:09 GMT
Distribution: bionet
Checksum: 27508     2

LOCUS       OSSRRLSA      167 bp ss-rRNA            RNA       19-AUG-1992
DEFINITION  Ossicaulis lignatilis ribosomal RNA (44LR3) large subunit, partial.
ACCESSION   M98601
KEYWORDS    large subunit ribosomal RNA.
SOURCE      Ossicaulis lignatilis (library: Duke culture collection 483) cDNA
            to rRNA.
  ORGANISM  Ossicaulis lignatilis
            Eukaryota; Fungi; Amastigomycota; Basidiomycetes.
REFERENCE   1  (bases 1 to 167)
  AUTHORS   Hibbett,D.S. and Vilgalys,R.
  TITLE     Phylogenetic relationships of the basidiomycete genus Lentinus
            inferred from molecular and morphological characters
  JOURNAL   Unpublished (1992)
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     rRNA            <1..>142
                     /note="Aligns to region between positions 371 to 597
                     inSaccharomyces cerevisiae large subunit nuclear rRNA."
                     /product="large subunit ribosomal RNA"
                     /citation=[1]
BASE COUNT       30 a     34 c     52 g     51 t
ORIGIN
        1 cgttgtccgg ggatcagcct tccttttggt tgtgtacttc ctggttgacg ggtcagcatc
       61 agttttgacc ggtggataag agtgagagga attgtggcat cttcgggtgt ttatagcctc
      121 ttgttgtata catcggttgg actgaggaac tcagcacgcc gaaggcc
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human immunodeficiency virus type 2 (22ENVB17) envelope (gp 41)
Date: 18 Aug 92 10:01:03 GMT
Distribution: bionet
Checksum: 56273     2

LOCUS       HIV2ENVB17    453 bp ss-RNA             VRL       18-AUG-1992
DEFINITION  Human immunodeficiency virus type 2 (22ENVB17) envelope (gp 41)
            fragment.
ACCESSION   M87121
KEYWORDS    envelope protein; glycoprotein.
SOURCE      Human immunodeficiency virus type 2 uncultured blood mononuclear
            cells (5/89 sample) RNA.
  ORGANISM  Human immunodeficiency virus type 2
            Viridae; ss-RNA enveloped viruses; Positive strand RNA virus;
            Retroviridae; Lentivirinae.
REFERENCE   1  (bases 1 to 453)
  AUTHORS   Gao,F., Yue,L., White,A.T., Pappas,P.G., Barchue,J., Hanson,A.P.,
            Greene,B.M., Sharp,P.M., Shaw,G.M. and Hahn,B.H.
  TITLE     Human infection by genetically-diverse SIVsm-related HIV-2 in West
            Africa
  JOURNAL   Nature (1992) In press
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     misc_feature    1..453
                     /note="PCR derived HIV-2 envelope (gp 41) fragment"
                     /note="putative"
                     /citation=[1]
BASE COUNT      154 a     75 c    112 g    112 t
ORIGIN
        1 gacgtggtca aaagacaaca ggaaatgttg cggctgaccg tctggggaac taaaaacctc
       61 cagactagag tcactgccat cgagaagtac ctaaaggacc aagcaaaact aaattcatgg
      121 ggatgtgcct ttagacaggt ttgccacact actgtgccat gggtgaacga cagtatgcag
      181 ccagagtggc aaaacatgac ttggcaacaa tgggaaaaac agattgcctt cttggaggat
      241 aatatcacag agctgttaga gcaagctcaa atacagcagg agaaaaatat gtatgagttg
      301 cagaaattaa atagctggga tgtttttggc aattggcttg atctcacctc ttgggtaaaa
      361 tatatctatt taggatttta tatagtagca ggagtaatag tgttaagaat agctatctat
      421 gtagtacaaa tgttaatgag acttaggaag ggc
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human chromosome 4 sequence-tagged site STS4-101
Date: 21 Aug 92 10:06:30 GMT
Distribution: bionet
Checksum: 52919     3

LOCUS       HUM4STS101    431 bp ds-DNA             PRI       21-AUG-1992
DEFINITION  Human chromosome 4 sequence-tagged site STS4-101
ACCESSION   M95214
KEYWORDS    STS; human chromosome 4; sequence tagged site.
SOURCE      Homo sapiens, clone C4-5 from Los Alamos National Laboratory
            chromosome 4 cosmid library, plate 4-1-176R, pos 5A; vector
            SuperCos 1 (Stratagene)
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 431)
  AUTHORS   Goold,R.D., diSibio,G., Dugaiczyk,A., Smith,K.A., Xu,H.,
            Magrane,G., Lang,D.B., Cox,D.R., Masters,S.B. and Myers,R.M.
  TITLE     Sequence-Tagged Sites for Human Chromosome 4
  JOURNAL   Unpublished (1992) see COMMENT for author address
  STANDARD  full automatic
COMMENT     PCR components: 25 ng of human genomic DNA, 10 pmol of each
            oligonucleotide, 200 micro-M dNTPs, 0.25 U Taq  polymerase (Cetus)
            in 10 micro-l of 50 mM KCl-20 mM  Tris-HCl, pH 8.3 (at room temp),
            2.5 mM MgCl-2.  Initial denaturation at 94degC for 1.5 min, then 30
            cycles of  94degC for 15 sec, 62degC for 23 sec, and 72degC for 30
            sec, followed by a final extension at 72degC for 3.5 min, using a
            Perkin-Elmer 9600 thermocycler. PCR-amplified product size 149 bp.
            Sequence submitted by:
            Human Genome Mapping Center
            Box 0925
            University of California San Francisco
            San Francisco, CA 94143-0925  USA
            Phone: (415) 502-1612   Fax: (415) 476-8391
            e-mail: hgmcprobes@cgl.ucsf.edu
FEATURES             Location/Qualifiers
     misc_feature    56..80
                     /label=Primer1
     misc_feature    complement(180..204)
                     /label=Primer2
BASE COUNT       96 a    103 c     85 g    104 t     43 others
ORIGIN
        1 gtgccacacg gtnttgtggg tagagnaagg gtctactagg agtgctattn gnaaagttca
       61 ttaatacctg ttagaagcca tccactggct cagataactt atgtccagat aggagcaaag
      121 agttcttttn atgagaactt ggtgtgcaaa tangccangn natagcagca tcttctntat
      181 ggcaaataga aatggagaga acaataanta catcgtctng gaggaaaaga aacctcantg
      241 tgtttggggc ggtgaagggc ganancancc gggaaantnc ntgccacctt cctttcggct
      301 gtcactccnc cgctccatcn ttnncttcag tcctcctnga cgcgtcattt attgganaac
      361 ccntcntnnt gctcncctct ttcncnccca cntttgcncg tancnncccc tnccntccnc
      421 cctctccann n
//