From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Haemophilus influenzae disulfide oxidoreductase (por) gene,
Date: 26 Jan 93 05:47:18 GMT
Distribution: bionet
Checksum: 18957 7

LOCUS       HEADSFO      1966 bp ds-DNA             BCT       25-JAN-1993
DEFINITION  Haemophilus influenzae disulfide oxidoreductase (por) gene,
            complete cds.
ACCESSION   M94205
KEYWORDS    disulfide oxidoreductase; methyl transferase.
SOURCE      Haemophilus influenzae (strain Rd) DNA.
  ORGANISM  Haemophilus influenzae
            Prokaryotae; Facultatively anaerobic gram-negative rods;
            Pasteurellaceae.
REFERENCE   1  (bases 1 to 1966)
  AUTHORS   Tomb,J.-F.
  TITLE     A periplasmic protein disulfide oxidoreductase is required for
            transformation of Haemophilus influenzae Rd
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 89, 10252-10256 (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     sig_peptide     621..689
                     /gene="por"
                     /evidence="EXPERIMENTAL"
                     /codon_start=1
                     /citation=[1]
     mat_peptide     690..1235
                     /function="disulfide-bond formation. Required for HI
                     transformation"
                     /product="disulfide oxidoreductase"
                     /gene="por"
                     /evidence="EXPERIMENTAL"
                     /codon_start=1
                     /citation=[1]
                     /note="The gene product, Por, contains the C..C motif
                     present in thioredoxin, PDI, DsbA and TcpG. Por restores
                     sensitivity to M13 to an E.coli strain deficient in DsbA
                     formation"
     CDS             344..610
                     /function="unknown"
                     /citation=[1]
                     /note="putative;  the product of this gene is undetectable
                     on SDS-PAGE.; ORF1"
                     /codon_start=1
                     /translation="MKCKRLNEVLELLQSYWSKDSDLSLMEILQKIANESGFQKPLNE
                     LTDEVIIYQLKMDGTDKYEPIPGLKKDYEEDFKTALLRARGIIK"
     CDS             621..1238
                     /gene="por"
                     /evidence="EXPERIMENTAL"
                     /product="disulfide oxidoreductase"
                     /codon_start=1
                     /translation="MKKVLLALGLGVSTLMSVNSFAADLQEGKQYVQVSQQASQQKEV
                     IEFFSFYCPHCYAFEMEYKIPQQVVDALPKDVKFKQYHVNFLGHQSENLTRAWALAMA
                     LGAESKVKSPLFEAAQKDALKSMDDIRAIFLSNGITAEQFDGGINSFAVNGLVNKQVN
                     AAEQFKVRGVPDFYVNGKFRVNPEGLNYDDFVKDYVQTVKGLLQK"
     CDS             1300..1644
                     /function="unknown, 58% identical to an untranslated ORF
                     in E.coli"
                     /citation=[1]
                     /note="putative;  the product of this gene is detectable
                     in both H.influenzae and E.coli and seems to partition to
                     the periplasmic space. a mutation in ORF3 causes a slight
                     decrease intransformation efficiency.; ORF3"
                     /codon_start=1
                     /translation="MAASFSVTRRFFDDKNYPRGFSRHGDYTIKESQVLEQYGQAFKA
                     LDLGEREPATKEEKDFVAFCRGERAAETFFEKTWNKYRTRINTKKRVYTLSSDVSEAA
                     SGGEDYPANKIR"
     CDS             1778..1966
                     /function="probably a tRNA methyl transferase."
                     /citation=[1]
                     /partial
                     /note="putative;  this partial ORF is 58% identical to an
                     E.coli tRNA methyl transferase gene, trnA.; ORF4"
                     /codon_start=1
                     /translation="MQLPISQYNELLQKKLEKLTALLHPFNAPDIQVFDSPTSHYAMR
                     AEFRIWHEQDDFYHIMFDQ"
BASE COUNT      654 a    325 c    380 g    607 t
ORIGIN      
        1 tctagagctg ttagcatgcc acttaatggc ccttgaaaat cgggcagttc atcggagaaa
       61 acaggaaagc caaattttgc atattctgtt tgatttcggt tagcgttaat tgaaatctga
      121 tgaatttgtg gttgtaagcg attaataata tgttcaatta aagattgttt acctaaaatc
      181 tgtaatcctt tatcttgccc gcccattcta cgagccttgc cacctgccaa aattactgcg
      241 cttattgtaa ttgtcatatt ttaatctatc attatttcaa gtatcatttg cctatttttt
      301 acgattaaaa aggaaatagc aatgaaataa aaaggaaata gcaatgaaat gtaagcgttt
      361 aaatgaagtt ttggaacttt tacagtctta ttggtctaaa gactcggatt tgagtttaat
      421 ggaaattttg caaaaaattg ccaatgaatc aggtttccaa aaaccattaa atgaattaac
      481 tgatgaagtg attatttatc agttaaaaat ggatggtaca gataaatatg agccaattcc
      541 tggtctcaaa aaagattacg aagaagattt taaaaccgca ttgctccgtg ctcgcggtat
      601 cattaaataa gaggaaagaa atgaaaaaag tattacttgc gttaggttta ggtgtcagca
      661 cgcttatgtc tgtaaatagt tttgccgcag atttacaaga aggcaaacaa tatgttcaag
      721 tgagtcaaca ggcttcacag caaaaagaag tgattgagtt tttctcattc tattgcccgc
      781 attgttacgc ctttgaaatg gaatacaaaa ttccacaaca agtcgtagat gctttaccaa
      841 aagatgtgaa atttaaacaa tatcatgtaa atttcttagg tcatcaatct gaaaacttaa
      901 cacgtgcttg ggcgttagca atggcattag gtgcagaaag taaagtaaaa tcaccattat
      961 ttgaagcggc tcaaaaggat gccttgaaat caatggatga tattcgagct atcttcttat
     1021 cgaatggcat aaccgccgag caatttgatg gtggcattaa tagttttgca gtaaatggtt
     1081 tagttaataa acaagtgaat gcagcagaac aatttaaagt gcgtggcgta cctgattttt
     1141 atgtaaatgg aaaattccgt gtaaaccctg aagggttaaa ttatgatgat ttcgtgaaag
     1201 attatgtgca aaccgtaaaa ggtttattgc aaaaataacg aaaaattggt ttaatgccag
     1261 ccctattttg taaatattaa tgaaagaaga gagaatatta tggctgcaag ttttagcgta
     1321 acgcgtcgtt tctttgacga caaaaactac ccacgtggtt tttcccgtca cggtgattac
     1381 acaattaaag aatcacaggt gcttgagcaa tatggtcaag cgttcaaagc attagactta
     1441 ggggaacgtg agccagcgac taaagaagaa aaagatttcg tcgcattttg ccgtggtgag
     1501 cgcgcagcag agactttctt tgaaaaaact tggaataaat atcgtactcg tattaacact
     1561 aaaaaacgtg tttacacctt atctagtgat gtgagtgaag ctgcctctgg cggtgaagat
     1621 tatccggcga ataagatacg ttaaataagg caggcggaag tctgcctttt gtttttgaat
     1681 agcttgtgaa atcacgcgta attcacggct gataacttta ttctcctatc tttgtatgaa
     1741 cgattcgtct tcatacaccc tatgaatact aatttctatg caattaccga tttctcaata
     1801 caacgaactg ctacaaaaaa aacttgaaaa attaaccgca cttttacacc cttttaatgc
     1861 accagatatt caagtttttg actctccaac aagtcattat gccatgcgtg cggagtttcg
     1921 tatttggcac gagcaagatg atttttacca tattatgttt gatcaa
//