From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Neisseria meningitidis class 5 outer membrane protein (opc) gene,
Date: 26 Jan 93 05:49:02 GMT
Distribution: bionet
Checksum: 16393 5

LOCUS       NGOOPC       1369 bp ds-DNA             BCT       25-JAN-1993
DEFINITION  Neisseria meningitidis class 5 outer membrane protein (opc) gene,
            complete cds.
ACCESSION   M80195
KEYWORDS    opc gene; outer membrane protein.
SOURCE      Neisseria meningitidis (strain ) DNA.
  ORGANISM  Neisseria meningitidis
            Prokaryotae; Gracilicutes; Scotobacteria; Neisseriaceae.
REFERENCE   1  (bases 1 to 1369)
  AUTHORS   Olyhoek,A.J., Sarkari,J., Bopp,M., Morelli,G. and Achtman,M.
  TITLE     Cloning and expression in Escherichia coli of opc, the gene for an
            unusual class 5 outer membrane protein from Neisseria meningitidis
  JOURNAL   Microb. Pathog. 11, 249-257 (1991)
  STANDARD  full automatic
REFERENCE   2  (sites)
  AUTHORS   Virji,M., Makepeace,K., Ferguson,D.J., Achtman,M., Sarkari,J. and
            Moxon,R.E.
  TITLE     Expression of the Opc protein correlates with invasion of
            epithelial and endothelial cells by Neisseria meningitidis
  JOURNAL   Mol. Microbiol. 6, 2785-2795 (1992)
  STANDARD  full automatic
REFERENCE   3  (sites)
  AUTHORS   Rosenqvist,E., Hoiby,E.A., Wedege,E., Kusecek,B. and Achtman,M.
  TITLE     The 5C protein of Neisseria meningitidis is highly immunogenic in
            humans and induces bactericidal antibodies
  JOURNAL   Unpublished (1993)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     repeat_region   join(147..156,193..202)
                     /rpt_type=DIRECT
                     /rpt_unit=147..156
                     /gene="opc"
     repeat_region   join(1239..1250,1254..1265)
                     /rpt_type=INVERTED
                     /rpt_unit=1239..1250
     repeat_region   join(160..172,208..220)
                     /rpt_type=DIRECT
                     /rpt_unit=160..172
                     /gene="opc"
     repeat_unit     203..215
                     /rpt_type=DIRECT
                     /gene="opc"
     TATA_signal     257..262
                     /gene="opc"
     -35_signal      279..283
                     /evidence="EXPERIMENTAL"
                     /note="non-optimal -35 used in meningococci"
     -10_signal      310..315
     RBS             366..369
                     /gene="opc"
     sig_peptide     386..442
                     /gene="opc"
                     /codon_start=1
     mat_peptide     443..1201
                     /product="outer membrane protein"
                     /gene="opc"
                     /evidence="EXPERIMENTAL"
                     /codon_start=1
     repeat_unit     1231..1242
                     /rpt_type=INVERTED
                     /gene="opc"
     repeat_unit     1246..1257
                     /rpt_type=INVERTED
                     /gene="opc"
     CDS             386..1204
                     /gene="opc"
                     /product="outer membrane protein"
                     /codon_start=1
                     /translation="MKKTVFTCAMIALTGTAAAAQELQTANEFTVHTDLSSISSTRAF
                     LKEKHKAAKHIGVRADIPFDANQGIRLEAGFGRSKKNIINLETDENKLGKTKNVKLPT
                     GVPENRIDLYTGYTYTQTLSDSLNFRVGAGLGFESSKDSIKTTKHTLHSSRQSWLAKV
                     HADLLSQLGNGWYINPWSEVKFDLNSRYKLNTGVTNLKKDINQKTNGWGFGLGANIGK
                     KLGESASIEAGPFYKQRTYKESGEFSVTTKSGDVSLTIPKTSIREYGLRVGIKF"
BASE COUNT      428 a    323 c    267 g    351 t
ORIGIN      
        1 aattccgcgc aggcgggaat ccagaccccc aacgcggcag gaatctatcg caaataaccg
       61 aaaccggacg aacctagatt cccgctttcg cggaatgacg gcagagtggt ttcagttgct
      121 cccgataaat gccgccatct caagtctcgt cattccctta aaacagaaaa ccgaaatcag
      181 aaacctaaaa tttcgtcatt cccataaaaa acagaaaacc aagtgagaat aacaattcgt
      241 tgtaaacaaa taactatttg ttaattttta ttaatatatg taaaatcccc ccccccgaaa
      301 gcttaagaat ataattgtaa gcgtaacgat tatttacgtt atgttaccat atccgactac
      361 aatccaaatt ttggagattt taactatgaa aaaaacagtt tttacatgtg ccatgattgc
      421 cctgaccggt actgccgccg ctgcacaaga gcttcaaacc gctaatgagt ttaccgtcca
      481 caccgacctc tcttccattt cttcaactcg tgctttcctg aaagaaaaac acaaagctgc
      541 caaacacatc ggcgtacgtg ctgatattcc ttttgatgcc aaccaaggca tccgcttgga
      601 agccggtttc gggcgcagca aaaaaaatat tattaatttg gaaacagatg agaacaagct
      661 gggtaagact aaaaatgtaa aactgcccac cggcgttcct gaaaaccgta tcgatcttta
      721 cacaggctac acctacaccc aaacgttaag tgattcttta aatttccgtg tgggtgccgg
      781 cttgggtttt gaatcttcaa aagacagcat taaaaccacc aagcatacgc ttcacagcag
      841 ccgtcagtcg tggttagcca aagttcacgc ggatttgctt tcccaactgg gtaacggctg
      901 gtatatcaac ccttggtctg aagtgaaatt tgacctcaat tcccgctata aattaaacac
      961 cggcgttacc aatctcaaaa aagacatcaa tcaaaaaacc aacggctggg gctttggatt
     1021 gggtgcaaat attggtaaaa aactgggcga atccgccagc atcgaggcgg ggccgttcta
     1081 caaacaacgc acttacaaag aatccggcga gtttagtgta acaaccaaga gtggcgacgt
     1141 atcgctcacc atcccgaaaa ccagtattcg tgaatacggc ttgcgcgtcg gcataaaatt
     1201 ctgatgattt gaaatcatca ttgtcacttt aaatgccaaa ccgcaaacta ttttggtttg
     1261 cggtttttac gtgaaatgaa tttgaatagc cgatgccgtc tgaaaatttc agacggcatt
     1321 tttccaatcg gttaaaatac gcggtttgtt ttttttagga acgggaatt
//