From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: phbACv=acetyl-CoA acyltransferase...orf 7 3' to phbB [Chromatium
Date: 12 Jan 93 05:48:17 GMT
Distribution: bionet
Checksum: 18433 10

LOCUS       M246906S2    3777 bp    DNA             BCT       11-JAN-1993
DEFINITION  phbACv=acetyl-CoA acyltransferase...orf 7 3' to phbB [Chromatium
            vinosum, strain D, Genomic, 7 genes, 3777 nt, segment 2 of 2] 
ACCESSION   S46675
KEYWORDS    .
SEGMENT     2 of 2
SOURCE      Chromatium vinosum strain D
  ORGANISM  Chromatium vinosum
            Unclassified.
REFERENCE   1  (bases 1 to 3777)
  AUTHORS   Liebergesell,M. and Steinbuchel,A.
  TITLE     Cloning and nucleotide sequences of genes relevant for biosynthesis
            of poly(3-hydroxybutyric acid) in Chromatium vinosum strain D.
  JOURNAL   Eur. J. Biochem. 209, 135-150 (1992)
  STANDARD  full automatic
COMMENT     This entry [NCBI gibbsq 115523] was created by the journal scanning
            component of NCBI/GenBank at the National Library of Medicine.
            This sequence comes from Fig. 2.  The authors begin their numbering
            at 3201.
FEATURES             Location/Qualifiers
     CDS             join(S46163:3181..3200,1..1165)
                     /note="beta-ketothiolase;  For the protein sequence (NCBI
                     gibbsq 115526):  Method: conceptual translation supplied
                     by author.  This sequence comes from Fig. 2."
                     /gene="phbACv"
                     /product="acetyl-CoA acyltransferase"
                     /codon_start=1
                     /translation="MSENIVIVDAGRSAIGTFGGSLSSLSATEIGTAVLKGLLARTGL
                     APEQIDEVILGQVLTAGVGQNPARQTTLHAGLPHSVPAMTINKVCGSGLKAVHLAMQA
                     IACGDADIVIAGGQESMSQSSHVLPRSRDGQRMGDWSMKDTMIVDGLWDAFNNYHMGT
                     TAENIAQKYGFTREQQDAFAAASQQKTEAAQKAGRFQDEIIPIEIPQRKGDPKVFDAD
                     EFPRHGTTAESLGKLRPAFSKDGSVTAGNASGINDGAAMVVVMKESKAKELGLKPMAR
                     LVAFASAGVDPAIMGTGPIPASTKCLEKAGWTPADLDLIEANEAFAAQAMSVNQDMGW
                     DLSKVNVNGGAIAIGHPIGASGARVLVTLLYEMQKRDAKKGLATLCIGGGQGVALAVE
                     RM"
     CDS             1312..1773
                     /note="Authors translation contains an extra Glu at
                     position 64 and is missing a Lys at position 152.
                     Description: orf 4 3' to phbA;  For the protein sequence
                     (NCBI gibbsq 115527):  Method: conceptual translation
                     supplied by author.  This sequence comes from Fig. 2.
                     Author-given protein sequence is in conflict with the
                     conceptual translation"
                     /codon_start=1
                     /translation="MNSERIIKKYPNRRLYDTEVSRYITLADVRDLVMSGQPFRVLDS
                     ANDSDITRSILLQIMLEEEETGGQPLFSANMLAQIIRFYGGTLQGTFARYLESSLDLF
                     AKQQQEVTKALTDNPFGTVTRLTQKNVEIWADLQDELMRAAGFPVAPRKKE"
     CDS             1870..2232
                     /note="Description: orf 5 5' to phbB;  For the protein
                     sequence (NCBI gibbsq 115528):  Method: conceptual
                     translation supplied by author.  This sequence comes from
                     Fig. 2."
                     /codon_start=1
                     /translation="MNTTDSLKTVNEWTNKSVERMTSFGELNVRLFEKLAARQMDAVN
                     LYMDHSMRLMKLATESKGYNDLFKGQVDATKELSERVMAESKATMQFFGDARDEYRVW
                     FEKSLNDVSEDLRKSVAV"
     CDS             2421..3161
                     /note="For the protein sequence (NCBI gibbsq 115529):
                     Method: conceptual translation supplied by author.  This
                     sequence comes from Fig. 2."
                     /gene="phbBCv"
                     /product="NADH-dependent acetoacetyl-CoA reductase"
                     /codon_start=1
                     /translation="MARIALVTGGIGGIGTSICTRLAKDGCTVVANCHPSEAAAAEEW
                     KQARAAEGFDIAVFTADVSSFDDSARMVREITEQVGPIDILVNCAGITRDKTFKKMEQ
                     AHWEAVINVNLNSVFNVTRQVWDGMLERGFGRIINISSVNGQRGQFGQANYSAAKAGM
                     HGFTMALAQEGASKGVTVNTISPGYVETAMTLAMNDDVRNSIISGIPMRRMAQPNEIA
                     AAIAFLAGDESGYMTGANLPVNGGLFMH"
     CDS             3311..3775
                     /partial
                     /note="Description: orf 7 3' to phbB;  For the protein
                     sequence (NCBI gibbsq 115530):  Method: conceptual
                     translation supplied by author.  This sequence comes from
                     Fig. 2."
                     /codon_start=1
                     /translation="MNAVMTDVRDLIRYCDDVLDAARFADYAPNGLQVEGERPLQRLV
                     SGVTASAALIEAAIAEHADAILVHHGWFWKNENPCLIGIKGQRARTLLSAGVSLIAYH
                     LPLDAHPELGNNATLGRRLDFIDMEPTALANGLLWAAIGSAHDACVLHGACLA"
BASE COUNT      747 a   1198 c   1170 g    662 t
ORIGIN      
        1 cgtcgacgcc ggccgcagtg ccatcggaac cttcggcggc agtctgtcgt cactctcggc
       61 caccgagatc ggcaccgccg tgctcaaggg gctgctggcg cgtaccggac tcgcgccgga
      121 acagatcgac gaggtgattc tcggccaggt gctgaccgcc ggcgtgggcc agaaccccgc
      181 ccgtcagacc acgctgcacg cggggctacc gcattcggtg ccggccatga ccatcaacaa
      241 ggtctgcggc agcggtctga aggcggtgca tctggcgatg caggccatcg cctgcgggga
      301 tgccgacatc gtcatcgccg gcggtcagga gagcatgagc cagtcctcgc acgtcctgcc
      361 gcgttcgcgc gacggtcagc gcatgggcga ctggtcgatg aaggacacca tgatcgtcga
      421 cggcctctgg gatgccttca acaactatca catgggcacc accgccgaga acatcgccca
      481 gaagtacggc tttacgcgcg agcagcagga cgccttcgcc gccgcctcgc agcagaagac
      541 cgaggccgcg cagaaggctg gccgcttcca ggacgagatc attccgatcg agattccgca
      601 gcgcaagggc gatccgaagg tgtttgatgc cgacgagttc ccgcgtcacg gcaccacggc
      661 cgagagtctg ggcaagctgc gtccggcctt ctcgaaggac ggcagcgtca cggcgggtaa
      721 cgcctccggc atcaacgacg gggcggccat ggtcgtggtg atgaaggagt ccaaggccaa
      781 ggaactgggt ctgaagccga tggcgcgtct ggtggccttc gccagcgccg gtgtcgatcc
      841 ggcgatcatg gggacgggcc cgatcccggc gtcgaccaag tgcctggaga aggccggctg
      901 gaccccggcg gatctggatc tgatcgaggc caacgaagcc ttcgccgcgc aggccatgtc
      961 ggtcaaccag gacatgggct gggatctgtc caaggtcaac gtcaacggcg gcgccatcgc
     1021 catcggtcat ccgatcggcg cctccggtgc gcgcgtgctc gtgaccctgc tctatgagat
     1081 gcagaagcgc gacgccaaga agggtctggc gacgctgtgc atcggcggcg gccagggcgt
     1141 ggcgctggcg gtcgagcgga tgtgagccgt cgtccgccgg tctgaatcgc cggcggaccg
     1201 agcctcctga atcgctccag gcactgaacg ccctgccgat cccggatcgg tggggcgttt
     1261 gcgcgcttgg ggtagacttg ccgaacgacc agccgaaccg cccgggtgcc catgaacagc
     1321 gagcgcatca tcaagaagta tccgaaccgc cgcctctacg acaccgaggt cagccgctat
     1381 atcaccctcg ccgatgtgcg cgatctggtg atgagcggac agcccttccg cgtcctcgac
     1441 agcgccaatg acagcgatat cacccgttcc atcctgctcc agatcatgct ggaggaggag
     1501 accggcggtc agccgctgtt cagcgccaac atgctggccc agatcatccg cttctacggc
     1561 ggcacccttc agggcacctt cgcccgctat ctggaatctt cactcgacct gttcgccaag
     1621 cagcaacagg aagtgaccaa ggcactcacc gacaatccct tcgggacggt gacacgcctg
     1681 actcagaaga acgtcgagat ctgggctgat ctccaggacg aactcatgcg cgcggctggc
     1741 tttccggtcg cgccgcgcaa gaaaaaagaa taatgaggat tgcgaaaatt gcgcttgacg
     1801 gccgtcggtc acagctttat tgtgcaatgc aacattgctg cactgcacaa accttacgga
     1861 gagatgatca tgaacaccac cgacagcctc aagaccgtca acgagtggac caacaagagc
     1921 gtcgagcgca tgaccagctt cggtgagctg aacgtgcgtc tgttcgagaa gctggccgcc
     1981 cgtcagatgg acgccgtgaa cctgtacatg gatcacagca tgcgcctgat gaagctggcc
     2041 accgagtcca agggttacaa tgacctcttc aagggtcagg tcgacgccac caaggaactg
     2101 agcgagcgcg tcatggccga gagcaaggcc accatgcagt tcttcggcga tgcccgcgac
     2161 gaataccgcg tgtggttcga gaagagcctg aacgacgtca gcgaagatct gcgcaagagc
     2221 gtcgccgtct aaagacgccg acctctgggc catcgcgatc cagggatgga tcgccattgg
     2281 tcatgcttcc ggatcggccg ggagcacgcc caatggaacc aacgcttcac cttgcctgcc
     2341 gcttggtagt aaagtggcct tgaagttcga cgacactgtt catcgttctc aatagttcca
     2401 aagattcctg gaggaacccc atggctcgta tcgcactcgt caccggcggc atcggcggca
     2461 tcggcacttc gatctgcaca cgcctggcaa aggatggctg caccgtcgtg gcgaactgcc
     2521 atccgtccga ggcggccgcc gccgaagagt ggaagcaggc ccgtgccgcc gaggggttcg
     2581 acatcgccgt cttcaccgct gacgtgtcct cgttcgacga cagcgcgcgc atggttcgcg
     2641 agatcacaga gcaggtcggt cccatcgaca tcctggtcaa ctgtgccggc atcacccgcg
     2701 acaagacctt caagaagatg gagcaggcgc actgggaggc cgtgatcaac gtcaacctca
     2761 acagcgtctt caacgtcacc cgtcaggtgt gggacgggat gctggagcgc ggcttcgggc
     2821 gtatcatcaa catctcgtcg gtcaacggtc agcgcggcca gttcggtcag gccaactatt
     2881 ccgccgccaa ggccggtatg cacggcttca ccatggctct ggctcaggag ggtgcgtcca
     2941 agggcgtgac cgtcaacacc atctcgcccg gctatgtcga gacggccatg accctggcga
     3001 tgaacgacga tgtgcgcaac agcatcatca gcggtattcc gatgcgtcgc atggctcagc
     3061 ctaatgagat cgccgccgcc atcgctttcc tggccggcga cgagagcggt tatatgacgg
     3121 gcgccaatct gccggtcaac ggcggtctgt tcatgcattg atttagatca taccgggccg
     3181 aatacaaaac actgacaatg gcccggtgtt tcaggatctc accgagtcct cctcgtctct
     3241 ctcatcatga gacgtttaca gcccggcgcc agccgggctt ttttttgtgt agaatcgaat
     3301 cgcccatccg atgaacgctg tgatgaccga cgtacgcgat ctgatccgct actgcgatga
     3361 cgtgctcgac gcggcgcgct tcgccgacta tgcgccgaat ggtttgcaag tggagggcga
     3421 acggccgctc cagcggctgg tgtcgggcgt gacggccagc gccgcgttga tcgaggccgc
     3481 gatcgcggag cacgccgacg ccattctggt ccatcatggc tggttctgga agaacgagaa
     3541 tccctgcctg atcggcatca aggggcagcg cgcaaggaca ttgctcagcg cgggtgtgag
     3601 tctgatcgcc tatcatctgc cgctcgatgc ccatcccgaa ctcggcaaca atgccacact
     3661 cggtcgccgg ctcgatttca tcgacatgga accgaccgca ctggccaatg gtctgctgtg
     3721 ggcggcgatt ggctcagccc atgacgcctg cgtccttcac ggagcatgtc tcgcatc
//