From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Plasmodium knowlesi erythrocyte binding protein (gamma) gene,
Date: 22 Aug 92 10:01:21 GMT
Distribution: bionet
Checksum: 14436    10

LOCUS       PFAERTHBPG   4467 bp ds-DNA             INV       22-AUG-1992
DEFINITION  Plasmodium knowlesi erythrocyte binding protein (gamma) gene,
            complete cds.
ACCESSION   M90695
KEYWORDS    erythrocyte binding protein.
SOURCE      Plasmodium knowlesi (strain Malayan H) late schizont DNA.
  ORGANISM  Plasmodium knowlesi
            Eukaryota; Animalia; Protozoa; Microspora; Microsporea;
            Microsporida; Haemosporina; Plasmodiidae.
REFERENCE   1  (sites)
  AUTHORS   Adams,J.H., Hudson,D.E., Torii,M., Ward,G.E., Wellems,T.E.,
            Aikawa,M. and Miller,L.H.
  TITLE     The Duffy receptor family of Plasmodium knowlesi is located within
            the micronemes of invasive malaria merozoites
  JOURNAL   Cell 63, 141-153 (1990)
  STANDARD  full staff_review
REFERENCE   2  (sites)
  AUTHORS   Fang,X., Kaslow,D.C., Adams,J.H. and Miller,L.H.
  TITLE     Cloning of the Plasmdium vivax receptor
  JOURNAL   Mol. Biochem. Parasitol. 44, 125-132 (1991)
  STANDARD  full staff_review
REFERENCE   3  (bases 1 to 4467)
  AUTHORS   Adams,J.H., Sim,B.K., Dolan,S.A., Fang,X., Kaslow,D.C. and
            Miller,L.H.
  TITLE     A family of erythrocyte binding proteins of malaria parasites
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 89, 7085-7089 (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     CDS             join(297..356,522..3474,3581..3659,3898..3974,4057..4100)
                     /standard_name="Duffy binding protein"
                     /product="erythrocyte binding protein"
                     /gene="gamma"
                     /codon_start=1
                     /translation="MEGKKKRPLFFLLVLLLSHKANNVLFERMNGILLLECENEYVKN
                     ENGYKLATGHHYMDNDQIERWLQGTDRSRRVKIEENVKYKYNVEELNTKYEQMKGKRI
                     NRILKESTYEAQNVADNNYIDDKANGEYKTDNKTNKGEGARNMVMLDYDISGSGQPDG
                     IIDNVVELLTEDEGNFLKNSSKGDDHPYRMKRKEKMSSGAINQIFLQNNVMDKCNDKR
                     KRGERDWDCPTEKDVCIPDRRYQLCMMEITNLVDTDTHFHSDIIFRKSYSRRRLIYDV
                     GGRGDLLLKKYNNVYSEDLCKDIKWSLQDFGDIIMGTDMEGIGYSLVVQNNLRSIFGT
                     GTSAELDRKKWWNDHKKDIWKAMILSVKEKNRYSAWNCKEDVQIKVEPQIYRWIREWG
                     RDYMSEFREQRRKLNEKCEDKLYYSTMLICTLPPCNNACKSYDEWITGKKKQWDVLST
                     KFSSVKKAQKIETENIARAYDILKQELNGFNEVTFENEINKRDKLYNYFCVCIVQEAR
                     KNTQENVKNVGSGVESKAPSSNPINEAVKSSSGEGKVQEDSAHRSVNEGEGKSSTNEA
                     DPGSQPGGPASRSVDEKAGVPALSAGQGHDKVPPAEAAATESAVPHSADKTPITATEE
                     NKQRTQVDGVAGGDGKAPGPTVSSDVPSVGGKDSGPSTPASHLAGENGEVHNGTDTEP
                     KEDGEKADPQKNIEVKGKQDTDDRSQGSLGPHTDERASLGETHMEKDTETTGGSTLTP
                     EQNVSVASDNGNVPGSGNKQNEGATALSGAESLESSESVHKTIDNTTHGLENKNGGNE
                     KDFQKHDFMNNDMLNDQTSSDHTSSDQTSSDQTSSDQTSSDQTSSDQTSSDQTSSDQT
                     SSDQTIDTEGHHRDNVRNPEIKSSEDMSKGDFMRNSNSNELYSHNNLNNRKLNRDQYE
                     HRDVKATREKIILMSEVNKCNNRTSLKYCNTIEDRMLSSTCSRERSKNLCCSISDFCL
                     NYFELYSYEFYNCMKKEFEDPSYECFTKGSSTGIVYFATGGAFLIILLLFASWNAASN
                     DYEEEATFDEFEEYCYNIHRTPQMPNDIEHMQQFTPLDYS"
     intron          357..521
                     /number=1
                     /cons_splice=(5'site:yes,3'site:yes)
                     /gene="gamma"
                     /evidence=EXPERIMENTAL
     exon            522..3474
                     /number=1
                     /function="erythrocyte binding domain"
                     /gene="gamma"
     intron          3475..3580
                     /number=2
                     /cons_splice=(5'site:yes,3'site:yes)
                     /label=intron2
     exon            3581..3659
                     /number=2
                     /function="transmembrane domain"
                     /gene="gamma"
     intron          3660..3899
                     /number=3
                     /cons_splice=(5'site:yes,3'site:yes)
                     /gene="gamma"
     exon            3898..3974
                     /number=3
                     /function="cytoplasmic domain"
                     /gene="gamma"
     intron          3975..4056
                     /number=4
                     /cons_splice=(5'site:yes,3'site:yes)
                     /gene="gamma"
     exon            4057..4100
                     /number=4
                     /function="cytoplasmic domain"
                     /gene="gamma"
BASE COUNT     1670 a    605 c    901 g   1291 t
ORIGIN
        1 aaaaaaaaaa aaatttttaa aagggggttt agatgtagta ataagaaatt gtaataaaga
       61 attgcccaca acagagtcac aacatttgct atgttttaca ctccctatat atgtaggttc
      121 atataattta gttctcgtac ttttcttttt cgtctctttt atatacatgc gctcaattgt
      181 ctgtgcttta catgttcagt tatatgtgta gaacacgtag ctacataatt tgctcatacg
      241 ccccccttaa tatgctttgt ataatataaa cacgtataca cctattttta aatacaatgg
      301 aaggaaaaaa aaaacgtcct ttattttttc tcctagtttt attattgtca cacaaggtac
      361 tgaaggacaa ttttttttac cagactaata aattatcatg aaggaaaaaa aaaaaaaaaa
      421 aaaaggggga ccatttaatt atactagaag aagggtggaa caaaatgaag aaagctaaga
      481 atcgtgatta gtgtaatctg ttctctttct ttttctttta ggctaataat gtattatttg
      541 aacgaatgaa tggaatcctt ttactagaat gcgaaaatga atacgtgaaa aatgaaaatg
      601 gttataaatt agctacagga catcattata tggacaatga tcaaatagaa cgatggttac
      661 aaggaactga tagaagcaga agagtgaaga ttgaggaaaa tgtaaaatat aaatataacg
      721 tagaggaact aaatacaaaa tatgaacaaa tgaaaggaaa aagaattaac cgcattttga
      781 aggaatccac ttacgaggcg caaaatgttg cagacaacaa ttatatagat gataaagcaa
      841 atggagaata taaaactgat aataaaacta ataaggggga aggtgcaagg aatatggtca
      901 tgttggatta tgatatatct ggcagtggcc agccagatgg gatcattgat aatgttgttg
      961 aattgttaac tgaggatgag ggaaattttc ttaaaaattc ctcaaaaggc gatgaccatc
     1021 cttatcgtat gaaacggaag gaaaaaatgt ctagtggtgc tattaatcaa atttttcttc
     1081 aaaacaatgt aatggacaag tgtaatgata agagaaagcg cggggaaaga gattgggact
     1141 gtccaactga gaaagatgtt tgcataccag atagaagata tcaattatgt atgatggaaa
     1201 ttacaaatct ggtagatacc gacacacatt ttcatagtga tataatattt cgaaaatcat
     1261 attcgagaag gagacttatt tatgatgttg gaggcagagg cgatttatta ttgaagaagt
     1321 ataacaacgt atatagcgaa gacttatgta aggatattaa atggagtttg caggattttg
     1381 gagatataat tatgggaact gatatggagg gtattgggta ttccctagta gtgcaaaaca
     1441 atttgcgcag catctttgga actggtacaa gtgccgaact ggatcgtaag aaatggtgga
     1501 atgatcataa aaaagatatt tggaaagcaa tgattttatc agttaaagaa aaaaatagat
     1561 attctgcatg gaattgtaaa gaagatgttc agataaaagt ggaaccgcag atatatagat
     1621 ggattcgaga atggggaaga gattatatgt cagaattccg cgaacaacga cggaaactaa
     1681 atgaaaaatg tgaagataaa ttatactatt ctactatgtt aatatgtact ttgcctccat
     1741 gcaataatgc ctgtaaatca tatgatgaat ggataacagg aaaaaaaaaa cagtgggatg
     1801 ttttgtcaac aaaattttca agcgtaaaga aggcacaaaa aatcgagacg gaaaatatcg
     1861 caagagctta tgatatacta aaacaggaat taaatggatt taatgaagtg acgtttgaga
     1921 atgaaattaa caaacgtgat aaattatata attatttttg cgtttgtatc gttcaggagg
     1981 ctagaaagaa tacccaggaa aatgtgaaaa atgtaggaag tggtgttgaa tctaaggctc
     2041 ccagttcgaa tccgataaat gaggctgtaa aaagtagtag cggggagggg aaggttcagg
     2101 aggattctgc acacagaagt gtgaacgaag gtgaaggtaa gtctagcaca aatgaagctg
     2161 atccaggttc ccaaccaggt ggtcctgctt ctcgtagtgt agatgaaaag gcaggtgttc
     2221 ctgctctatc agctggtcaa ggtcatgata aagttccccc tgctgaagct gctgctacag
     2281 aatcggctgt tccacattca gcagacaaaa ctccaattac agcaacagaa gaaaataagc
     2341 aaagaacgca ggtggatggt gttgcaggtg gagatggtaa ggctcctggt ccaactgttt
     2401 cttccgatgt tcctagtgtt gggggtaagg atagtggtcc cagtacccct gcgtcccatc
     2461 tcgctgggga aaatggtgaa gttcataatg gcactgatac tgaacctaag gaagatggtg
     2521 agaaggcaga tccccaaaaa aatatagaag taaagggtaa gcaagacaca gatgataggt
     2581 cacaggggtc acttgggcca catactgatg aaagagcatc tttgggggaa actcatatgg
     2641 agaaggatac agaaaccaca ggaggtagta ctctcactcc cgaacagaat gttagtgttg
     2701 cttctgataa tggtaatgtt cctggatctg gcaataagca aaatgagggt gcaactgcgt
     2761 tgagtggagc tgaaagtttg gaatcaagcg aaagtgtaca taaaactatt gataatacaa
     2821 ctcacggttt agaaaataaa aatggaggaa acgaaaagga ttttcagaag cacgatttta
     2881 tgaataatga catgttgaat gatcaaacaa gttctgatca cacaagttca gaccaaacaa
     2941 gttctgatca aacaagttca gaccaaacaa gttcagatca aacaagttca gaccaaacaa
     3001 gttcagatca aacaagttca gaccaaacaa gttcagatca aactatagat acagaaggac
     3061 atcacaggga taatgtcaga aatcctgaaa taaagagttc ggaagatatg agtaaagggg
     3121 attttatgag aaattcaaat agtaacgaat tatatagtca taataatttg aataatcgta
     3181 aattaaatag agaccaatac gaacacagag atgtcaaagc aacaagggaa aaaattatcc
     3241 ttatgtctga agtaaacaaa tgcaataata ggacatcctt aaaatactgt aacactatag
     3301 aagacagaat gttatcgagc acttgttcaa gggagaggag taaaaattta tgttgttcaa
     3361 tatcggattt ttgtttgaat tattttgagc tctattctta tgaattttac aattgcatga
     3421 aaaaggaatt tgaagatcca tcatacgagt gctttacaaa aggaagctcc acaggtatag
     3481 aggaaaagat gatcaataga caaagatgtt acttacattg aaaataaatt cattttagga
     3541 atgttataaa tttttttgta atcaatattc ttttttgcag gcatagttta ttttgccacg
     3601 gggggagcgt ttctgataat actgttgcta tttgcctcat ggaatgcagc ctcgaatgag
     3661 taaccaaaga ataatataaa aattagaata agaataagaa ttagagtgga agctagatta
     3721 acaattaaaa taaaaaataa aatagaaagt gctgttaatg cacaattaat tctatatatt
     3781 ccatgtgtga aattttaagg agagtaaaaa tgtgtaatca ttatatgcat gcacgtatac
     3841 atatatagac atatatgtac caatatataa taaatgcaca cttccttgtt cgtacagtta
     3901 tgaagaagaa gctacatttg atgagtttga ggaatactgt tataatattc acagaacccc
     3961 tcaaatgcct aacggtaatt caaatttcaa aggcaaaatt tcatttaaga agaaatatta
     4021 aatcattctg cattattctt tttttttttt ctttagatat tgagcacatg caacaattta
     4081 cacccctgga ttattcataa tactacttgg gtaagtaagg agaattgcga ttgtacagct
     4141 gatggaattt ttgtcatttt gcttgatgca ataaatgaga aggtatatga agtttccgca
     4201 agaatgattt aacaccacta tgacaattac ttgcaccatg cgtctttatt taaatatcta
     4261 aattagaatt aatttttttg tatttgtaat aagttaatac aagtgtattt ccttttattg
     4321 gcttgtagga tgtgtagtcc tttagccaat agtagaaaaa ttcgtttagg cgaattgtat
     4381 aattttcctt ttttttttta tttgaacttt ttatttttct tatattgaca aaaaaaaaaa
     4441 taccatttca ataatttgaa taataca
//