From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: S.pyogenes PCP gene for pyrrolidone carboxylyl peptidase
Date: 12 Sep 92 10:03:53 GMT
Distribution: bionet
Checksum: 23362     4

LOCUS       STRPCPG      1337 bp ds-DNA             BCT       12-SEP-1992
DEFINITION  S.pyogenes PCP gene for pyrrolidone carboxylyl peptidase
ACCESSION   X65717
KEYWORDS    oxoprolyl peptidase.
SOURCE      Streptococcus pyogenes DNA.
  ORGANISM  Streptococcus pyogenes
            Prokaryotae; Firmicutes; Firmibacteria; Gram-positive cocci;
            Streptococcaceae.
REFERENCE   1  (bases 1 to 1337)
  AUTHORS   Cleuziat,P. and Robert-Baudouy,J.
  TITLE     Molecular characterization of pcp the structural gene encoding the
            pyrrolidone carboxylyl peptidase from streptococcus pyogenes
  JOURNAL   Mol. Microbiol. 6, 2051-2063 (1992)
  STANDARD  full automatic
COMMENT     *source: strain=D471;
            From EMBL    entry SPPCPG;  dated 27-AUG-1992.
FEATURES             Location/Qualifiers
     -10_signal      222..227
     -35_signal      245..250
     prim_transcript 257..986
                     /gene="PCP"
                     /evidence=EXPERIMENTAL
     RBS             271..277
     CDS             285..932
                     /EC_number="3.4.19.3"
                     /product="pyrrolidone carboxylylate peptidase"
                     /gene="PCP"
                     /evidence=EXPERIMENTAL
                     /codon_start=1
                     /translation="MKILVTGFDPFGGEAINPALEAIKKLPATIHGAEIKCIEVPTVF
                     QKSADVLQQHIESFQPDAVLCIGQAGGRTGLTPERVAINQDDARIPDNEGNQPIDTPI
                     RADGKAAYFSTLPIKAMVAAIHQAGLPASVSNTAGTFVCNHLMYQALYLVDKYCPNAK
                     AGFMHIPFMMEQVVDKPNTAAMNLDDITRGIEAAIFAIVDFKDRSDLKRVGGATH"
     terminator      964..984
                     /evidence=EXPERIMENTAL
BASE COUNT      406 a    260 c    252 g    419 t
ORIGIN
        1 aacaaaataa aagaacttac ctattttcca tccaaaatgt ttagcaatca tcatctgcaa
       61 ggcaacgtat tgcatggcat tgatgtgatg agcaactaat atgtcattag aacgttgcgt
      121 caaactagca tctaaataaa gatcgaaatg cagttatcaa aaatgcaagc tcctatcggc
      181 ccttgtttta attattactc acattgcctt aatgtattta cttgcttatt attaactttt
      241 ttgctaagtt agtagcgtca gttattcatt gaaaggacat tattatgaaa attcttgtaa
      301 caggctttga tccctttggc ggcgaagcta ttaatcctgc ccttgaagct atcaagaaat
      361 tgccagcaac cattcatgga gcagaaatca aatgtattga agttccaacg gtttttcaaa
      421 aatctgccga tgtgctccag cagcatatcg aaagctttca acctgatgca gtcctttgta
      481 ttgggcaagc tggtggccgg actggactaa cgccagaacg cgttgccatt aatcaagacg
      541 atgctcgcat tcctgataac gaagggaatc agcctattga tacacctatt cgtgcagatg
      601 gtaaagcagc ttatttttca accttgccaa tcaaagcgat ggttgctgcc attcatcagg
      661 ctgggcttcc tgcttctgtt tctaatacag ctggtacctt tgtttgcaat catttgatgt
      721 atcaagccct ttacttagtg gataaatatt gtccaaatgc caaagctggg tttatgcata
      781 ttccctttat gatggaacag gttgttgata aacctaatac agctgccatg aacctcgatg
      841 atattacaag aggaattgag gctgctattt ttgccattgt cgatttcaaa gatcgttccg
      901 atttaaaacg tgtagggggc gctactcact gactgtgacg ctactaaacc tattttaaaa
      961 aaacagagat atgaactaac tctgtttttt ttgtgctaaa aatgaaagac ctagggaaac
     1021 ttttcatcgg tctttctcaa ttgtcatctt aatctaatac tacttctaac atcagcgggt
     1081 atagtttgcc agtaattaag aaacgttgtt gatctaaatg agcaatccca ttcaaaacat
     1141 taaggtcagg gtaatgggac ttatcaagat ttaaggcttt taacaaagga ctaatatcat
     1201 aggtggctac cacctttcca gaatcaggtt ggagtttgac aatagtattg gtttgccaaa
     1261 tattggcata gagataacca tctacatact ctaattcgtt aagcattgag atagggacac
     1321 tttctatagc aactagt
//