From: arun@natinst.com (Arun Gupta)
Newsgroups: bionet.jobs
Subject: Seeking a biochemist/molecular biologist position
Date: 5 Oct 92 20:54:43 GMT
Organization: National Instruments, Austin, TX
NNTP-Posting-Host: falcon.natinst.com


			      RACHNA GUPTA
			11700 Metric Blvd, # 1412
	        	    Austin, TX 78758

			   Tel. (512)-794-5783
		     e-mail:  arun@falcon.natinst.com
	
Personal:	I have authorization to work in USA.

OBJECTIVE
To obtain employment in a laboratory or technical setting that would allow
me to use my skills as a biochemist/ molecular biologist.

EDUCATION
M. S. Molecular Biology and Biotechnology.  July 1992.
G. B. Pant University of Agriculture and Technology(GBPUAT).
GPA  4.97/5.0    FIRST RANK IN THE UNIVERSITY.

B. S.  Chemistry (Honors).  July 1989.
St. Stephens College, Delhi, India.
Percentage 71.0.

WORK EXPERIENCE
Lab Instructor for General Biochemistry. Fall 1990.
Department of Molecular Biology and Genetic Engineering, GBPUAT

Teaching Assistant. Spring 1991.
Department of Molecular Biology and Genetic Engineering, GBPUAT
Assisted in Microbiology.

Teaching Assistant. Fall 1991.
Department of Molecular Biology and Genetic Engineering, GBPUAT
Assisted in Immunological Techniques.


TECHNICAL SKILLS
-Immunoelectrophoresis, gel diffusion, PAGE etc.
-ELISA, RIA, Western blotting etc.
-Conjugation of Progesterone (steroid hormone) with proteins (Alkanline
 phosphatase)
-Affinity purification of antibodies from cell culture supernatants and ascites
-Chromatography (Gel filtration, Ion Exchange)
-Spectroscopy
-Genetic Engineering -  Isolation of Plasmids (mini and bulk preparation)
                        CsCl density gradient Centrifugation
                        Agarose Gel Electrophoresis

PROJECTS
Biochemistry:   -Synthesis of protected amino acid derivatives and dipeptides
                -Solid phase synthesis and purification of linear and branched
                 peptides (10 to 60 amino acids)
                -purification of oligonucleotides
Genetic Engg:   -DNA footprinting
Immunology:     -Hybridoma Technology
                -Embryo Culture


COMPUTER SKILLS
I have taken introductory courses in Computers and Fortran 77 programming
Currently I am also learning C language and Database programming


GRADUATE COURSE WORK
Master's Thesis:
 A MILK BASED DOT-BLOT IMMUNO ASSAY TECHNIQUE FOR BOVINE PREGNANCY DIAGNOSIS.

Progestrone level in biological fluids provided an effective way of monitoring 
ovarian activity in farm animals.  Pregnancy can be diagnosed by measuring 
progesterone levels as early as 18 days after mating or insemination.  A high
titre antiserum was raised in rabbits against 11-alpha-hydroxy progestrone 
hemisuccinate (antigen). 11-alpha-hydroxy progestrone hemisuccinate alkaline 
phosphatase conjugate was prepared and its enzyme activity & immuno-reactivity 
were tested.  Reliability of solid, liquid and dot phase ELISA in confirming 
pregnancy status of dairy cows through milk was tested and dot ELISA was found 
to be the most simple and sensitive test.

Microbiology, Immunological Techniques, Principles and Practices of Genetic 
Engineering, Genetic Engineering in Crop Productivity, Principles of Molecular 
Biology, Radiotracer Techniques, Cell Structure and Function, General 
Biochemsitry, Biochemical Methods, Modern Analytical Techniques in Biology,
Introduction to Computers and Programming


AWARDS AND HONORS
-Won an award during National Science Week held by GBPUAT for my working model
 on Trasduction, Transformation and Conjugation (1990)
-Won National Mathematics Olympiad Prize for excellence in Mathematics (1985)


SCHOLARSHIPS:
-Merit Scholarship from Department of Biotechnoloy.
-Central Board of Secondary Education (CBSE) Merit Scholarship (1986)
 for excellent performance in Senior Secondary.
-National Scholarship Prize from Directorate of Education (1986).


MEMBERSHIPS AND ACTIVITIES
        -Society of Biological Chemists (India)
        -Editor of Scinfo Magazine, Department of Biotechnology, GBPUAT
        
INTERESTS
I want to work in protein chemistry (isolation, purification, analytical
methods).  Preferred areas are immunology, receptor research and protein/
DNA interactions.

REFERENCES:     Available upon request.
Newsgroups: bionet.jobs