From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: P.vulgaris adenosine triphosphatase (atpa) gene, complete cds.
Date: 1 Sep 92 10:03:16 GMT
Distribution: bionet
Checksum: 22437    12

LOCUS       PHVATPA      5986 bp ds-DNA             PLN       01-SEP-1992
DEFINITION  P.vulgaris adenosine triphosphatase (atpa) gene, complete cds.
ACCESSION   M64246
KEYWORDS    ATPA; adenosine triphosphatase.
SOURCE      P.vulgaris DNA.
  ORGANISM  Phaseolus vulgaris
            Eukaryota; Plantae; Embryobionta; Magnoliophyta; Magnoliopsida;
            Rosidae; Fabales; Fabaceae.
REFERENCE   1  (bases 1 to 5986)
  AUTHORS   Chase,C.D. and Ortaga,V.M.
  TITLE     Organization of ATPA coding and 3' flanking sequences associated
            with cytoplasmic male sterility in Phaseolus vulgaris L
  JOURNAL   Curr. Genet. 22, 147-153 (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     CDS             1..1527
                     /function="oxidative phosphorylation"
                     /standard_name="ATPA"
                     /product="adenosine triphosphatase"
                     /gene="atpa"
                     /note="putative"
                     /codon_start=1
                     /translation="MEFSSRAAELTTLLESRMTNFYTNFQVDEIGRVVSVGDGIARVY
                     GLNEIQAGEMVEFASGVKGIALNLENENVGIVVFGSDTAIKEGDLVKRTGSIVDVPAG
                     KAMLGRVVDALGVPIDGRGALSDHERRRVEVKAPGIIERKSVHEPMQTGLKAVDSLVP
                     IGRGQRELIIGDRQTGKTAIAIDTILNQKQMNSRATSESETLYCVYVAIGQKRSTVAQ
                     LVQILSEANALEYSILVAATASDPAPLQFLAPYSGCAMGEYFRDNGMHALIIYDDLSK
                     QAVAYRQMSLLLRRPPGREAFPGDVFYLHSRLLERAAKRSDQTGAGSLTALPVIETQA
                     GDVSAYIPTNVISITDGQICLETELFYRGIRPAINVGLSVSRVGSAAQLKAMKQACGS
                     LKLELAQYREVAAFAQFGSDLDAATQALLNRGARLTEVLKQPQYAPLPIEKQILVIYA
                     AVNGFCDRMPLDKIPQYERDILTTIKPELLQSLKGGLTSERKIELEKFLKEKAKNYTL
                     "
     CDS             1524..2153
                     /note="ORF-209"
                     /note="putative"
                     /codon_start=1
                     /translation="MKKKLMMKDRILRGFFFTLLLCMLKNGMYSLVSDFDLYTILAWW
                     IPTFYMDDVGASSQSSLPPAEPQAPEPLPPAEPQAPDLSHTLLDDNTRRAELDERAGF
                     HFVGLSEEKKEKVLLAQVQIERAIEKALLSDGYSRDELSQRSKRDEIRGFLFYRNGKL
                     LSIKKYESYVEEVKFGTHRSQPYQDLIHAISDSYLFLKKVKKIKRWERG"
     misc_feature    1862..2160
                     /note="Sequence similarity with soybean mitochondrial DNA
                     sequenced as part of a rearrangement identified in tissue
                     culture. 86.9% identity in 289 nucleotide overlap"
                     /note="putative"
     misc_feature    2165..2166
                     /note="Junction between repeat 2 (containing ATPA coding
                     and orf-209 sequences) and CMS-unique sequences"
                     /note="putative"
     CDS             3364..3660
                     /note="ORF-98"
                     /note="putative"
                     /codon_start=1
                     /translation="MDHFSRGCHPVLPMSLAINKVANTNKTSSQRTIQAHYRRNFKTL
                     VLREPKHSFKGGIPDEGTLGEPPTPTIVHVRSILDLTNCPSYLLYVLDSPSLSQ"
     misc_feature    3536..3557
                     /note="Sequence similarity with higher plant chloroplast
                     DNA encoding tRNA alanine intron; 95.3/95.8% identity in
                     192/190 nucleotide overlap"
                     /note="putative"
     CDS             5172..5891
                     /note="ORF-239"
                     /note="putative"
                     /codon_start=1
                     /translation="MFLPFNPQKIFGRLSSTLSRKIIVRISIVVVSVFTIGYLFCLVF
                     GIDLSLIFGNQLVRSFRVLLSRLLGWEVPVLVFFVGLELDSSLHMQDPAGGQPAANPA
                     AEQPSNVPSGGSTSKGTWWKKWIPCFTPQTEEGGHSVSPPEPTRNWVLDVDLPREDPS
                     ETIDTLRRKCAIIIQSFCQNNARDDLTFDDIAQRLVLETKGTTIGATREDFQRLLGEL
                     QNPAESDVYLRVLKALEKHPR"
     misc_feature    5901..5902
                     /note="Junction between CMS-unique sequence and repeat 1"
                     /note="putative"
     misc_feature    5901..5986
                     /note="Sequence similarity with plant mitochondrial
                     cytochrome b coding sequences; 95.2-98.8% identitiy in an
                     84 nucleotide overlap may continue beyond end of file"
                     /note="putative"
BASE COUNT     1684 a   1309 c   1372 g   1621 t
ORIGIN
        1 atggaattct cttccagagc tgcggaacta accactctat tagaaagtag aatgaccaac
       61 ttttacacaa attttcaagt ggatgagatc ggtcgagtgg tctccgttgg agatgggatt
      121 gcacgtgttt atggattgaa cgagattcaa gctggggaaa tggttgaatt tgccagcggt
      181 gtgaaaggaa tagcgttgaa tcttgagaat gagaatgtcg gaattgtggt ctttggtagt
      241 gataccgcta taaaagaagg agatcttgtc aaacgcactg gatctattgt ggatgttcct
      301 gcgggaaagg ctatgctagg gcgtgtggtc gacgcgttgg gagtacctat tgatggaaga
      361 ggggctctaa gcgatcacga gcgaagacgt gtcgaagtga aagcccctgg tattattgaa
      421 cgtaaatctg tgcacgagcc tatgcaaaca gggttaaaag cggtagatag cctggttcct
      481 ataggccgtg gtcaacgaga acttataatc ggggaccgac aaactggaaa aacagctatt
      541 gctatcgata ccatattaaa tcaaaagcaa atgaactcaa gggccacctc agagagtgag
      601 acattgtatt gtgtctatgt agcgattgga cagaaacgct caactgtggc acaattagtt
      661 caaattcttt cagaagcgaa tgctttagaa tattccattc ttgtagcagc caccgcttcg
      721 gatcctgcac ctctgcaatt tctggcccca tattctgggt gtgccatggg ggaatatttc
      781 cgcgataatg gaatgcacgc attaataatc tatgatgatc ttagtaaaca ggccgtggca
      841 tatcgacaaa tgtcattatt gttacgccga ccaccaggcc gtgaggcttt cccaggcgat
      901 gttttctatt tacattcccg tctcttagaa agagccgcta aacgatcgga ccagacaggt
      961 gcaggtagct tgaccgcctt acccgtcatt gaaacacaag cgggagacgt atcggcctat
     1021 attccaacca atgtgatctc cattactgat ggacaaatat gtttggaaac agagctcttt
     1081 tatcgcggaa ttagacctgc tattaacgtc ggcttatctg tcagtcgcgt cgggtctgcc
     1141 gctcagttga aagctatgaa acaagcctgc ggtagtttaa aactggaatt ggcacaatat
     1201 cgcgaagtcg ccgcctttgc tcaatttggc tcagaccttg atgctgcgac tcaggcatta
     1261 ctcaatagag gtgcaaggct tacagaagta ctgaaacaac cacaatatgc accacttcca
     1321 attgaaaaac aaattctagt catttatgca gctgtcaatg gattctgtga tcgaatgcca
     1381 ttagataaaa ttcctcaata tgaaagagac attctaacga ctattaaacc tgaattacta
     1441 caatcactaa aaggtggact aactagcgaa agaaaaatag aactagaaaa attcttaaaa
     1501 gaaaaagcaa aaaattacac attatgaaaa aaaagcttat gatgaaagat aggattttac
     1561 gaggattctt ttttactctc ttgctttgta tgttaaaaaa tggaatgtat tcgttggtat
     1621 ctgattttga tttatatacc atccttgctt ggtggattcc caccttttat atggacgacg
     1681 tgggcgccag ctcacagtcg tccttgcccc ctgcagagcc tcaagcccct gagcccttgc
     1741 cccctgcaga gcctcaagcc cctgaccttt cccatacttt actggatgac aacactcgtc
     1801 gcgccgaact agatgagcgc gcggggtttc attttgtggg gttgtcggaa gagaagaaag
     1861 aaaaagtgct tttggcacaa gtccaaatag aaagggccat tgagaaagct ctgctctccg
     1921 acgggtattc ccgggatgag ctttctcaaa ggagcaaacg agatgagatt aggggctttt
     1981 tgttctaccg taatgggaaa cttctttcta ttaagaaata cgagtcttat gtggaagaag
     2041 tcaaatttgg gacccaccgc agccagccat atcaagatct tatccatgcc atctctgatt
     2101 cctatctttt tttaaagaaa gtaaaaaaaa taaagaggtg ggaacggggg tgaaacagat
     2161 tgggctcaca gaatacttca gggctggcca cgtacctgtc aagaactgta taaaaaagga
     2221 ccgagaacta cataaggtct gagacggact tccttgaagg gagtctagtt atgtaggcag
     2281 tggccgttcc ggttgctcga ccaaagccgg tcaataagac agatccggat cctagtgctt
     2341 tcgagatggt tcgatcgcta acaaagacaa gcatgggaaa cagcagactc ccgcaactcc
     2401 aagagctcaa gcctaaagcc ttagtaacgc gcatcctcat tgctcgcgta aagcaagcgt
     2461 ataaggttta ttgatggaca ttcctataga aactatttcc cgcttgggac gtagttgttt
     2521 ccgttctgac ctacctttcc tcagccgctt agcgctgtga caaagacaga tgtgaaaatc
     2581 gaagactgac actggatcct taaacacctc aatcaatagc gtccgtgata atgttcggtc
     2641 tagccccttt ttttcttgtt ctgaggaaaa atagttcata ataggccagc caggactagt
     2701 gtgatcctaa ccggtcttat tctgacttac actattctat tttccgggat tgatgtccgc
     2761 ttggaaagga gtaaggttta ggctttaccc agttgaactc gaggttggta ataaactgaa
     2821 gctgctcagt cttctagcat agcggagtta gatatatcga agaatgaact tagccttcaa
     2881 ccacaaatgg ggtcctttag gcagccgatt gcccgtttgc ttccactgac ttacgtcgtg
     2941 aacattttta ttaatcaata gaattcctct tcgatcccat cctatgagcc ctgcgagctc
     3001 acgagtcgtc agtgatggtc agtcactcgg aggacgaccg gcctgaacta accggaacgc
     3061 agcgagctat caaacaagaa gtcgtgaact ccagaaagga aagaaagaac tccagcaaag
     3121 atagcataga cgggtactgc tacttgaaag cctaaggaaa atagcaagac tgacaacttc
     3181 ctcacccctt acttctcttc cattttcatt gtatcctttt tgagttgaag aaaggccctg
     3241 tcttccaatc tttctttcgt actcccttct ttctatatta catcaattcc ttttaacggc
     3301 caagaaaccg ctctgcttgt cggagataag gtcagtacgg aggtaggaca tgctgtcgag
     3361 aagatggatc atttttctag gggttgccat cccgtccttc caatgtccct cgcgattaac
     3421 aaagtagcta atactaataa gacttcgagt caacgaacga ttcaagctca ttacagaagg
     3481 aactttaaaa ctcttgtatt gagagaacca aagcactctt tcaaaggtgg gatcccagat
     3541 gagggaaccc taggagagcc gccgactcca actatcgtcc atgtacgatc tatactagat
     3601 ctgaccaact gcccatctta cctcctctac gttcttgaca gcccatcttt gtctcagtag
     3661 agtctttcag tggcatgttt cggtcctctt ccccattatt tagaaaaagt gagccaccca
     3721 gaggctggct gacaggaagg ctttcaaact acctagcttt tactcgctcg aaagcattag
     3781 caatttaagg cgaaagtaat ctttcatttt tctactgggc ttaaaaagga ctagaaatgg
     3841 ctcgcagaaa taagagatcc aattccaact tccattgaaa cataggagct gaacattctt
     3901 ccacagggaa ggcagaacgc atgacagaag atatgacagg gcttgcggaa gcactacaaa
     3961 gggcaggagt ggattctctc ttattgtatg tgtatgagta tgatcaacaa gaaagccata
     4021 acagaaatga taagaaggtc ttccatcaat catacaaaag cagcttcagt agcctaatac
     4081 cagcacaagt cgaggggcgt aagcgtttac ctttacctgt ttaatcaatc cctgggatac
     4141 taggcccact ctttagcaca ccataagatc gggtatatat aagggcccga gggagaaggg
     4201 gtgtcttagt caaattcctc gggagagtct gattagctcc tattgggttc taggccgacg
     4261 gttctattga cagcgtaggc tttgtttact tcataggaga ggatttgacg atcgtagtat
     4321 gatggattcc ctctctgtta cacgggggag taggatttcc taatctttca cctgtttacc
     4381 attaggttag gtcctcgttt cattccgtct agcgccttaa gttctgtgct aaggtgagct
     4441 ctagttcagt tcctcggatt gagatagaga gcctaccgac ccctttactc aataggacta
     4501 gctatagaaa aagtctctat agagaaagtc aggtgtgaat gattggcttg tcttgtctaa
     4561 ggctgaccgt acctagtgct tagttagtcg tttctctttt ctttgctttg aaagcgagaa
     4621 agatgatgtt ggaaagcggt taggagagca ggtacagatg gatgattgcc ctattgtgtt
     4681 aggcatggaa tttctagaca gcgtgaatgc cgtaccaatc ccatgtgcca acacactagc
     4741 catcctcggc gagaagaatg gtcctgcgga aagggaaaag agatagctcc ccctgcgcga
     4801 atcttcatgg aagtcttaat ataccacatt cttagatact agattgagtg tctctagctc
     4861 ttgtaaacat ctctctcgaa tgtctagctg gagaatcaat gtcagagagt aagagatagc
     4921 tcactcgcgg aagagaataa tagatcagat ccattggcgg gggtgactaa ccatggttcg
     4981 gctctcgccc taaggttacg atccgttggg ttgggaaacc aacctggatc cactttcccc
     5041 caagatccat accgattgag aaggagatca agttgaaaac aaccgagatc ggatctctca
     5101 cggaaattgt gaggccccaa tgtggtcatt acttatgtct ttcttgtacc caacttgaac
     5161 agaaaaaaaa catgttcctc ccattcaacc cacaaaaaat tttcggaaga ttgagttcaa
     5221 ctctgtcgcg aaagattata gtgcgaattt ctatcgttgt tgtttccgta ttcaccatag
     5281 gctatttgtt ctgtcttgta ttcggcattg atttgagtct tatttttgga aatcaattgg
     5341 tcaggtcgtt ccgcgttctc ttaagtcggc ttcttggttg ggaggttcct gttcttgttt
     5401 tttttgttgg tttggaactg gattccagct tgcacatgca ggatccagca ggaggtcaac
     5461 ctgctgccaa tcctgcagcg gagcaaccct ccaacgtgcc ttcaggcgga tcgacctcaa
     5521 aaggtacatg gtggaagaaa tggattcctt gtttcacccc ccaaacagaa gagggaggac
     5581 attctgtttc gcctcctgaa ccaacaagaa actgggtatt ggacgttgat cttccaaggg
     5641 aggatccgtc cgagacaatt gacactctta ggagaaagtg tgcgattatt attcaatcat
     5701 tctgtcaaaa taatgctaga gacgacttaa cctttgatga tatcgcacaa cgcctggttt
     5761 tggaaacaaa gggtacaacc ataggtgcta caagagagga cttccaacgt ctgcttggcg
     5821 aattacaaaa tccagcagag agcgacgtct atcttcgggt tttaaaagcc ctggagaagc
     5881 atccccgcta atggggcaac cgttactatt ggacaaattc ctccttttgt tttcttcttg
     5941 ttctttgcca taacgcccat tccgggacga gttggaagag gaattc
//