HDTenEightyP/NetNews huggingface dataset:
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Lentinus velutinus ribosomal RNA (37LR0R) large subunit, partial.
Date: 19 Aug 92 10:01:57 GMT
Distribution: bionet
Checksum: 29445     2

LOCUS       LENRRLSAW     238 bp ss-rRNA            RNA       19-AUG-1992
DEFINITION  Lentinus velutinus ribosomal RNA (37LR0R) large subunit, partial.
ACCESSION   M98658
KEYWORDS    large subunit ribosomal RNA.
SOURCE      Lentinus velutinus (library: Duke culture collection 795) cDNA to
            rRNA.
  ORGANISM  Lentinus velutinus
            Eukaryota; Fungi; Eumycota; Basidiomycotina; Hymenomycetes;
            Agaricales; Tricholomataceae.
REFERENCE   1  (bases 1 to 238)
  AUTHORS   Hibbett,D.S. and Vilgalys,R.
  TITLE     Phylogenetic relationships of the basidiomycete genus Lentinus
            inferred from molecular and morphological characters
  JOURNAL   Unpublished (1992)
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     rRNA            <1..>238
                     /note="Aligns to region between positions 70 to 306
                     inSaccharomyces cerevisiae large subunit nuclear rRNA."
                     /product="large subunit ribosomal RNA"
                     /citation=[1]
BASE COUNT       59 a     47 c     69 g     63 t
ORIGIN
        1 aaggattccc ctagtaactg cgagtgaagc gggaaaagct caaatttaaa atctggcagt
       61 ctttggctgt ccgaattgta ttctggagaa gcgtcttccg cgctggaccg tgtacaagtc
      121 tcttggaata gagcgtcata gagggtgaga atcccgtctt tgacacggac taccagtgct
      181 ttgtgatgcg ctctcaaaga gtcgagttgt ttgggaatgc agctcaaaat gggtggtg
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human chromosome 4 sequence-tagged site STS4-96
Date: 21 Aug 92 10:11:23 GMT
Distribution: bionet
Checksum: 19152     3

LOCUS       HUM4STS96     410 bp ds-DNA             PRI       21-AUG-1992
DEFINITION  Human chromosome 4 sequence-tagged site STS4-96
ACCESSION   M95252
KEYWORDS    STS; human chromosome 4; sequence tagged site.
SOURCE      Homo sapiens, clone C4-156 from Los Alamos National Laboratory
            chromosome 4 cosmid library, plate 4-1-2R, pos 6A; vector SuperCos
            1 (Stratagene)
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 410)
  AUTHORS   Goold,R.D., diSibio,G., Dugaiczyk,A., Smith,K.A., Xu,H.,
            Magrane,G., Lang,D.B., Cox,D.R., Masters,S.B. and Myers,R.M.
  TITLE     Sequence-Tagged Sites for Human Chromosome 4
  JOURNAL   Unpublished (1992) see COMMENT for author address
  STANDARD  full automatic
COMMENT     PCR components: 25 ng of human genomic DNA, 10 pmol of each
            oligonucleotide, 200 micro-M dNTPs, 0.25 U Taq  polymerase (Cetus)
            in 10 micro-l of 50 mM KCl-20 mM  Tris-HCl, pH 8.3 (at room temp),
            2.5 mM MgCl-2.  Initial denaturation at 94degC for 1.5 min, then 30
            cycles of  94degC for 15 sec, 62degC for 23 sec, and 72degC for 30
            sec, followed by a final extension at 72degC for 3.5 min, using a
            Perkin-Elmer 9600 thermocycler. PCR-amplified product size 317 bp.
            Sequence submitted by:
            Human Genome Mapping Center
            Box 0925
            University of California San Francisco
            San Francisco, CA 94143-0925  USA
            Phone: (415) 502-1612   Fax: (415) 476-8391
            e-mail: hgmcprobes@cgl.ucsf.edu
FEATURES             Location/Qualifiers
     misc_feature    18..42
                     /label=Primer1
     misc_feature    complement(310..334)
                     /label=Primer2
BASE COUNT      103 a    104 c     54 g    142 t      7 others
ORIGIN
        1 tcaaaaaata tcccagccca caaatttatc tgtccacatc tcttcagctt tggtgctacc
       61 atccttgtct atgccactga catctttnng gtaatagtat ggcaatagct tctgaacatt
      121 ctccttgntt ttctttggga gcccctaaaa tctattcttc attcagcaat ctgagtgaac
      181 tttctacaat gtaaataaga taatgttaag ctccttcttg aaacttacca atggcttctc
      241 tttgnacttg gaatcaattg ngaacatctt cttctttcct aaatgggctt ctatgatcta
      301 tcccaagcta gttctctaac ctcactatat cctgccaccc ctatttnggg cacactggct
      361 ggtctttttt tatcttcttc aagtccanca aactccttca aaactcagag
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human dinucleotide repeat polymorphism at the D5S356 locus.
Date: 15 Aug 92 10:02:03 GMT
Distribution: bionet
Checksum: 65181     1

LOCUS       HUMD5S356X    194 bp ds-DNA             PRI       15-AUG-1992
DEFINITION  Human dinucleotide repeat polymorphism at the D5S356 locus.
ACCESSION   M98786
KEYWORDS    dinucleotide repeat polymorphism.
SOURCE      Homo sapiens DNA.
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 194)
  AUTHORS   Polymeropoulos,M.H.M.D.., Xiao,H. and Merril,C.R.
  TITLE     Dinucleotide repeat polymorphism at the D5S356 Locus
  JOURNAL   Hum. Mol. Genet. (1992) In press
  STANDARD  simple staff_entry
FEATURES             Location/Qualifiers
     repeat_region   49..106
BASE COUNT       80 a     53 c     28 g     33 t
ORIGIN
        1 aaaatgggat aacaaataaa tggatgctgg ttcacataca aagcaaaaca cacacacaca
       61 cacacacaca cacacacaca cacacacaca cacacacaca cacacaggtc aaaattaatc
      121 tgaaaatctc tgaaatcact cggaatgtac agttttattt atagaccagc ttgcgcgaac
      181 ctggatggca ggcc
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human immunodeficiency virus type 2 (FOENVC33) envelope protein
Date: 18 Aug 92 10:01:58 GMT
Distribution: bionet
Checksum: 32078     2

LOCUS       HIVENVC33     453 bp ss-RNA             VRL       18-AUG-1992
DEFINITION  Human immunodeficiency virus type 2 (FOENVC33) envelope protein
            fragment.
ACCESSION   M87094
KEYWORDS    envelope protein.
SOURCE      Human immunodeficiency virus type 2 uncultured blood mononuclear
            cells (3/90 sample) RNA.
  ORGANISM  Human immunodeficiency virus type 2
            Viridae; ss-RNA enveloped viruses; Positive strand RNA virus;
            Retroviridae; Lentivirinae.
REFERENCE   1  (bases 1 to 453)
  AUTHORS   Gao,F., Yue,L., White,A.T., Pappas,P.G., Barchue,J., Hanson,A.P.,
            Greene,B.M., Sharp,P.M., Shaw,G.M. and Hahn,B.H.
  TITLE     Human infection by genetically-diverse SIVsm-related HIV-2 in West
            Africa
  JOURNAL   Nature (1992) In press
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     misc_feature    1..453
                     /note="PCR derived HIV-2 envelope fragment"
                     /gene="env"
                     /note="putative"
                     /citation=[1]
BASE COUNT      172 a     78 c     93 g    110 t
ORIGIN
        1 gacgtggtca gaagacaaca agaattgttg cgactgaccg tctggaggac taaaaacctc
       61 cagactagag tcactgctat cgagaagtac ctaaaggacc aggcgcagct aaattcatga
      121 agatgcgcgt tcagacaggt ctgccacacc actgtagaat ggccaaatag tactctcaca
      181 cctgactaga acaatatgac ttggcaggag taagaaagac aggttgattt cctagaggca
      241 aatataacac aattattaga agaagcacaa attcaacaag aaaagaacat gtatgagcta
      301 caaaaactaa ataactagga tatatttggc aactggtttg accttacttc ttagattaag
      361 tatatacagt atggagtctt catagtagta agtataatac tgttaagaat aataatctat
      421 gtagtacaaa tgttagctaa gttaagtaaa gga
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human chromosome 4 sequence-tagged site STS4-369
Date: 21 Aug 92 10:09:34 GMT
Distribution: bionet
Checksum: 13455     3

LOCUS       HUM4STS369    429 bp ds-DNA             PRI       21-AUG-1992
DEFINITION  Human chromosome 4 sequence-tagged site STS4-369
ACCESSION   L00824
KEYWORDS    STS; human chromosome 4; sequence tagged site.
SOURCE      Homo sapiens, clone p4-364 from a chromosome 4 plasmid library;
            vector pBluescript II KS + (Stratagene)
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 429)
  AUTHORS   Goold,R.D., diSibio,G., Dugaiczyk,A., Smith,K.A., Xu,H.,
            Magrane,G., Lang,D.B., Cox,D.R., Masters,S.B. and Myers,R.M.
  TITLE     Sequence-Tagged Sites for Human Chromosome 4
  JOURNAL   Unpublished (1992) see COMMENT for author address
  STANDARD  full automatic
COMMENT     PCR components: 25 ng of human genomic DNA, 10 pmol of each
            oligonucleotide, 200 micro-M dNTPs, 0.25 U Taq  polymerase (Cetus)
            in 10 micro-l of 50 mM KCl-20 mM  Tris-HCl, pH 8.3 (at room temp),
            2.5 mM MgCl-2.  Initial denaturation at 94degC for 1.5 min, then 30
            cycles of  94degC for 15 sec, 62degC for 23 sec, and 72degC for 30
            sec, followed by a final extension at 72degC for 3.5 min, using a
            Perkin-Elmer 9600 thermocycler. PCR-amplified product size 198 bp.
            Sequence submitted by:
            Human Genome Mapping Center
            Box 0925
            University of California San Francisco
            San Francisco, CA 94143-0925  USA
            Phone: (415) 502-1612   Fax: (415) 476-8391
            e-mail: hgmcprobes@cgl.ucsf.edu
FEATURES             Location/Qualifiers
     misc_feature    55..76
                     /label=Primer1
     misc_feature    complement(228..252)
                     /label=Primer2
BASE COUNT      167 a     63 c     73 g    124 t      2 others
ORIGIN
        1 gatcttaaaa acaatgtacc aaaaaaataa aaaataaaat aaaataaaaa agaagcccga
       61 ctcaaaatgc cacacatttt agaattccat ttatatgaag tgtccaggtt aggaaaatcc
      121 ataaagatag aaagctgatg agtgttagcc aggaactgag agaaagaggg aatagcaagt
      181 aactgctaat gggaatgggg tttcttnngg ggtgataaaa atgttctaga attagctagt
      241 ggtaatgatt gcataacctt gtgaatatac taaaaaccac tggattgtat attttaaaat
      301 agtaaaattt atgttatgtg aattatatct caatttaaaa ataaattcag cttatctatt
      361 aggttctcta ggcaaaactt ccttacttct aagggtactc ccagcactcc ttctccaatt
      421 acactactg
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human chromosome 4 sequence-tagged site STS4-109
Date: 21 Aug 92 10:06:37 GMT
Distribution: bionet
Checksum: 59773     3

LOCUS       HUM4STS109    438 bp ds-DNA             PRI       21-AUG-1992
DEFINITION  Human chromosome 4 sequence-tagged site STS4-109
ACCESSION   M95271
KEYWORDS    STS; human chromosome 4; sequence tagged site.
SOURCE      Homo sapiens, clone C4-160 from Los Alamos National Laboratory
            chromosome 4 cosmid library, plate 4-1-2R, pos 10A; vector SuperCos
            1 (Stratagene)
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 438)
  AUTHORS   Goold,R.D., diSibio,G., Dugaiczyk,A., Smith,K.A., Xu,H.,
            Magrane,G., Lang,D.B., Cox,D.R., Masters,S.B. and Myers,R.M.
  TITLE     Sequence-Tagged Sites for Human Chromosome 4
  JOURNAL   Unpublished (1992) see COMMENT for author address
  STANDARD  full automatic
COMMENT     PCR components: 25 ng of human genomic DNA, 10 pmol of each
            oligonucleotide, 200 micro-M dNTPs, 0.25 U Taq  polymerase (Cetus)
            in 10 micro-l of 50 mM KCl-20 mM  Tris-HCl, pH 8.3 (at room temp),
            2.5 mM MgCl-2.  Initial denaturation at 94degC for 1.5 min, then 30
            cycles of  94degC for 15 sec, 62degC for 23 sec, and 72degC for 30
            sec, followed by a final extension at 72degC for 3.5 min, using a
            Perkin-Elmer 9600 thermocycler. PCR-amplified product size 124 bp.
            Sequence submitted by:
            Human Genome Mapping Center
            Box 0925
            University of California San Francisco
            San Francisco, CA 94143-0925  USA
            Phone: (415) 502-1612   Fax: (415) 476-8391
            e-mail: hgmcprobes@cgl.ucsf.edu
FEATURES             Location/Qualifiers
     misc_feature    29..52
                     /label=Primer1
     misc_feature    complement(127..152)
                     /label=Primer2
BASE COUNT       88 a    120 c     57 g    138 t     35 others
ORIGIN
        1 tcacaggcca tttggaattg tctttttagg actttccaat tcacaccctt tgnccatttt
       61 cctattggtt tctgttgtct ttttcatatt aattttagag ctgtttatat atttattata
      121 ggttattaac cattaagtat catctgcaga aaaaagtaca aaaattattt nccaatattt
      181 taaagttgta tgacatattt nggtgcaatt ttatttnngg gaaatacaat taaatatgtc
      241 ttttatggtt tttntnctct tttggggnga cagagcctca ctcctgtcac ccagtcttng
      301 agtgcagnng nanccacccc gccctcactg canccccccc cctcccccgg ntcannccgn
      361 ctctnccnnc ccccagcccc cccngccgcn canccttnca ncncncnncn cccccccgac
      421 tcccntcctc ntcngctn
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: H.sapiens gene for CD59 protein, exon 3
Date: 22 Aug 92 10:00:48 GMT
Distribution: bionet
Checksum: 26039     3

LOCUS       HUMCD59EX3   1285 bp ds-DNA             PRI       22-AUG-1992
DEFINITION  H.sapiens gene for CD59 protein, exon 3
ACCESSION   Z14114
KEYWORDS    CD59 protein.
SOURCE      Homo sapiens DNA.
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 1285)
  AUTHORS   Tone,M.
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
REFERENCE   2  (sites)
  AUTHORS   Tone,M., Walsh,L.A. and Waldmann,H.
  TITLE     Gene structure of human CD59 and demonstration that discrete mRNAs
            are generated by alternative polyadenylation
  JOURNAL   J. Mol. Biol. (1992) In press
  STANDARD  full staff_review
COMMENT     *source: tissue=blood;
            From EMBL    entry HSCD59EX3;  dated 24-JUL-1992.
FEATURES             Location/Qualifiers
     intron          <1..560
                     /partial
     exon            561..662
                     /number=3
     intron          663..>1285
                     /partial
BASE COUNT      355 a    240 c    296 g    394 t
ORIGIN
        1 gaattctttt tagggggata accatttgct taattggggt tcaaaattag tgttccctcg
       61 catcaaatca attgcagatg ttcatctatt aatactaatc tgtgatgaca ttttacatat
      121 ttcattttga aaatgtattt tcacgcagat aggtattgcc agacattgat catcaaccca
      181 cgaggatgtg atttaattgg gcatattcat tgcttggaag acacttatag aattctgttt
      241 gtttctcggt aatttcttct taacacatca ttacattgca gactaatctc cttccagttg
      301 caggttagga ggaagcttct caattgcaca gttagatgga tattcaaatg tggatatttc
      361 ctttgtgctt gcatcagggt ccaaaaaatg ttgctggaac tgtagtttga atttggaaag
      421 atataactgc tatacatttg tgcgggagtg gaagtatacc acaagttgct gactttgggc
      481 ccatattaat ggagatggtg ggctgccagg ggacaagtca gtgctgcttt aagagatcct
      541 gactttcttc ctgattctag gtcatagcct gcagtgctac aactgtccta acccaactgc
      601 tgactgcaaa acagccgtca attgttcatc tgattttgat gcgtgtctca ttaccaaagc
      661 tggtaagagc ctcccctgtc tgtctcctaa atgtaatggg gtaataagtg cctgggaaaa
      721 aaattgtgcc actgtaatcc tcattaggct tgcatcaagt aatagctacc atttattagc
      781 gtcaggtgtg tattgggcta agcactttct tcatacattt taaatttaat gttttgcact
      841 tctaagggtg atcttttatt tccatttgat aaatgtggaa atggaggttc agattaagca
      901 agaaattatg gttaagtaac cgactcagga attgaacatg gaagtccatc tgattccaaa
      961 gcttgtgctc ttttacagca gccctgtgct gcctcccaga ccttggagaa cctactgtgt
     1021 gcccagcaga gggttaggag ctagggatgc aaagataaag aaaggacaca tacaattaaa
     1081 attatgacaa aggcctgttt ggctgtccta aatgtcatta tatatgcctc tgtctcattc
     1141 catggggtat ctggtatatt cagagtcctg aggagactca agctgagctc aaaggacacg
     1201 taggaggtaa gagggctaag aagggaaggg tgtgcctggg aggggcaaca gcaggtgcca
     1261 gggctcggta gcctcagagc aaggt
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human chromosome 4 sequence-tagged site STS4-127
Date: 21 Aug 92 10:06:46 GMT
Distribution: bionet
Checksum: 09754     3

LOCUS       HUM4STS127    351 bp ds-DNA             PRI       21-AUG-1992
DEFINITION  Human chromosome 4 sequence-tagged site STS4-127
ACCESSION   M95280
KEYWORDS    STS; human chromosome 4; sequence tagged site.
SOURCE      Homo sapiens, clone C4-94 from Los Alamos National Laboratory
            chromosome 4 cosmid library, plate 4-1-1R, pos 4D; vector SuperCos
            1 (Stratagene)
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 351)
  AUTHORS   Goold,R.D., diSibio,G., Dugaiczyk,A., Smith,K.A., Xu,H.,
            Magrane,G., Lang,D.B., Cox,D.R., Masters,S.B. and Myers,R.M.
  TITLE     Sequence-Tagged Sites for Human Chromosome 4
  JOURNAL   Unpublished (1992) see COMMENT for author address
  STANDARD  full automatic
COMMENT     PCR components: 25 ng of human genomic DNA, 10 pmol of each
            oligonucleotide, 200 micro-M dNTPs, 0.25 U Taq  polymerase (Cetus)
            in 10 micro-l of 50 mM KCl-20 mM  Tris-HCl, pH 8.3 (at room temp),
            2.5 mM MgCl-2.  Initial denaturation at 94degC for 1.5 min, then 30
            cycles of  94degC for 15 sec, 62degC for 23 sec, and 72degC for 30
            sec, followed by a final extension at 72degC for 3.5 min, using a
            Perkin-Elmer 9600 thermocycler. PCR-amplified product size 207 bp.
            Sequence submitted by:
            Human Genome Mapping Center
            Box 0925
            University of California San Francisco
            San Francisco, CA 94143-0925  USA
            Phone: (415) 502-1612   Fax: (415) 476-8391
            e-mail: hgmcprobes@cgl.ucsf.edu
FEATURES             Location/Qualifiers
     misc_feature    14..38
                     /label=Primer1
     misc_feature    complement(197..220)
                     /label=Primer2
BASE COUNT       83 a    101 c     73 g     92 t      2 others
ORIGIN
        1 cgacctctgc ctcccaaagt gaacgtgata gtttatccct gtggaaaagg tgagaagcag
       61 ggcctgggac tctgggacac atctgctgat acagagcttc ttcggagaca ctggtaccag
      121 ctgctggacc tgcctcattt gtgggcaggt aacagaaaac ttgcctactg atgtttctcc
      181 cgtcctttga cctccccatt ccaatgcaat actacctgag cactatttcc aaaattcgtt
      241 ctgttcaagt cgtccttctt caataaatca tttgntggcc ccacagacag actttggaat
      301 aaagcccagt ccctgccatc taccagcctc atctagggag nctcccactg t
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human chromosome 4 sequence-tagged site STS4-434
Date: 21 Aug 92 10:10:22 GMT
Distribution: bionet
Checksum: 01953     3

LOCUS       HUM4STS434    424 bp ds-DNA             PRI       21-AUG-1992
DEFINITION  Human chromosome 4 sequence-tagged site STS4-434
ACCESSION   L00860
KEYWORDS    STS; human chromosome 4; sequence tagged site.
SOURCE      Homo sapiens, clone p4-434 from a chromosome 4 plasmid library;
            vector pBluescript II KS + (Stratagene)
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 424)
  AUTHORS   Goold,R.D., diSibio,G., Dugaiczyk,A., Smith,K.A., Xu,H.,
            Magrane,G., Lang,D.B., Cox,D.R., Masters,S.B. and Myers,R.M.
  TITLE     Sequence-Tagged Sites for Human Chromosome 4
  JOURNAL   Unpublished (1992) see COMMENT for author address
  STANDARD  full automatic
COMMENT     PCR components: 25 ng of human genomic DNA, 10 pmol of each
            oligonucleotide, 200 micro-M dNTPs, 0.25 U Taq  polymerase (Cetus)
            in 10 micro-l of 50 mM KCl-20 mM  Tris-HCl, pH 8.3 (at room temp),
            2.5 mM MgCl-2.  Initial denaturation at 94degC for 1.5 min, then 30
            cycles of  94degC for 15 sec, 62degC for 23 sec, and 72degC for 30
            sec, followed by a final extension at 72degC for 3.5 min, using a
            Perkin-Elmer 9600 thermocycler. PCR-amplified product size 188 bp.
            STS sequence contains a simple sequence tandem repeat (CA).
            Sequence submitted by:
            Human Genome Mapping Center
            Box 0925
            University of California San Francisco
            San Francisco, CA 94143-0925  USA
            Phone: (415) 502-1612   Fax: (415) 476-8391
            e-mail: hgmcprobes@cgl.ucsf.edu
FEATURES             Location/Qualifiers
     misc_feature    100..122
                     /label=Primer1
     misc_feature    complement(263..287)
                     /label=Primer2
BASE COUNT      107 a     98 c     87 g    123 t      9 others
ORIGIN
        1 gatcgcggga gacaggttta ggaggggcca caccaatcgg ctcccagtcc cggctggcag
       61 gggaggcaga ggcctgggtt gcacctgtgc tcctctgggg aaacctggga gcattgctaa
      121 cccggggtcc ggtagaggga attcctagcc tggcgttttt agattacgat atacatatnt
      181 atatacacac acacacacac acacacacac acacacacac acgattaana tgctatatat
      241 tatgtatttc ttgtaaatca aatcgctctg tcattatatt agcaactttt atctccctag
      301 caaatattnt gtcttcaaat ctctttcgta tgagacttat ggagatacac tagtttactt
      361 tgttagtttg nggctggtat aactttccct atctttncag nttnaatgnn cctctgtcct
      421 atat
//
From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Methanothermus fervidus methyl coenzyme M reductase II (mrtB) gene,
Date: 19 Aug 92 10:02:44 GMT
Distribution: bionet
Checksum: 16617     3

LOCUS       MEFMRTB       600 bp ds-DNA             BCT       19-AUG-1992
DEFINITION  Methanothermus fervidus methyl coenzyme M reductase II (mrtB) gene,
            5' end.
ACCESSION   M99219
KEYWORDS    methyl coenzyme M reductase II;
            methyl viologen-reducing hydrogenase-polyferredoxin.
SOURCE      Methanothermus fervidus DNA.
  ORGANISM  Methanothermus fervidus
            Prokaryotae; Archaeobacteria; Methanobacteriales;
            Methanothermaceae.
REFERENCE   1  (bases 1 to 600)
  AUTHORS   Steigerwald,V.J., Stroup,D., Hennigan,A.N., Pihl,T.D. and
            Reeve,J.N.
  TITLE     Methyl coenzyme M reductase II genes are closely linked to the
            methyl viologen-reducing hydrogenase-polyferredoxin operon in the
            genomes of Methanobacterium thermoautotrophicum and Methanothermus
            fervidus
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     CDS             225..>600
                     /product="methyl coenzyme M reductase II"
                     /gene="mrtB"
                     /codon_start=1
                     /translation="MAIYEDKIDLYDANGKLLDENVPLEAISPLKNPTIGKIVNDVKR
                     SVAVNLAGIENSLKKAALGGKANFIPGRELDLDIVENAEIIAEKIKKMVQVDENDDTN
                     VKMINNGQQLLVQVPTIRIERAA"
BASE COUNT      239 a     63 c     97 g    201 t
ORIGIN
        1 taattttatt ttttcttttt ctccatgatt attactaatt atttgttatt tatgatttat
       61 ttaacatttt cttttttatt tttttagtaa aggatataaa cagtttttaa catagcttca
      121 aaaaacatct ggatgctgat gaatatattt gtgattttaa attatgatgt tggccaaaaa
      181 ttttattatt aataaaaaat tacataaaaa tatgaggtga caaaatggca atttatgaag
      241 acaaaataga tttgtacgat gctaacggaa aattgttaga tgaaaacgta ccattagaag
      301 caataagtcc tctaaaaaat ccaactattg ggaaaattgt taacgatgta aaaagatcag
      361 tggctgttaa tttagcaggt atagaaaaca gtcttaaaaa agcagcatta ggtggaaaag
      421 ctaattttat tcctggtagg gaattagatt tagacattgt agaaaatgct gagataatcg
      481 cagaaaaaat taaaaaaatg gttcaagttg atgaaaacga tgacacaaac gttaaaatga
      541 taaataatgg gcaacaatta cttgtacaag ttccaaccat aagaattgaa agagctgcag
//