From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: C.maltosa formaldehyde resistance (fahR) gene, complete cds.
Date: 28 Jan 93 05:49:28 GMT
Distribution: bionet
Checksum: 20589 5

LOCUS       YSAFAHR      2258 bp ds-DNA             PLN       27-JAN-1993
DEFINITION  C.maltosa formaldehyde resistance (fahR) gene, complete cds.
ACCESSION   M58332
KEYWORDS    formaldehyde resistance.
SOURCE      Candida maltosa (strain VSB-899) DNA.
  ORGANISM  Candida maltosa
            Eukaryota; Fungi; Eumycota; Deuteromycotina.
REFERENCE   1  (bases 1 to 2258)
  AUTHORS   Sasnauskas,K., Jomantiene,R., Januska,A., Lebediene,E., Lebedys,J.
            and Janulaitis,A.
  TITLE     Cloning and analysis of a Candida maltosa gene which confers
            resistance to formaldehyde in Saccharomyces cerevisiae
  JOURNAL   Gene 122, 207-211 (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     CAAT_signal     314..319
                     /gene="ADH1"
     exon            453..476
                     /number=1
                     /gene="ADH1"
     intron          477..713
                     /number=1
                     /gene="ADH1"
     exon            714..1835
                     /number=2
                     /gene="ADH1"
     polyA_signal    1914..1922
                     /gene="ADH1"
     CDS             join(453..476,714..1835)
                     /gene="ADH1"
                     /note="encoding formaldehyde resistance"
                     /codon_start=1
                     /translation="MSESTVGKPITCKAAVAWEAAKPLSIEDVTVAPPKRHEVRIKLY
                     DTGVCHTDAYTLSGVDPEGAFPVILGHEGAGIVESIGEGVTNVKVGDHVIALYTPECG
                     ECKFCKSGKTNLCGKIRATQGKGVMPDGTSRFTCKGKEILHFMGCSTFSQYTVVADIS
                     VVAINPKAEFDKACLLGCGITTGYGAATITANVQKGDNVAVFGGGIVGLSVIQGCAER
                     GAAQIILVDISDKKEEWGQKLGATAFVNPTKLPEGTTIVDKLIEMTDGGCDFTFDCTG
                     NVGVMRNALEACHKGWGTSVIIGVAAAGKEISTRPFQLVTGRTWKGAAFGGVKGRSQL
                     PGIVNNYLDGKLKVEEFITHREPLAAINKAFEEMHAGDCIRAVVDLS"
BASE COUNT      714 a    401 c    406 g    737 t
ORIGIN      
        1 agaaagaaaa agaaaaagca agcgatagtc aacacaaaga gaatatctga gtttaagtgt
       61 gtcaacgtta catcaaaata gagaattagt aatcaagata gaaccaatac ttttctaaac
      121 aatattgcgg gtgagatact tcgtgattat atgagtttaa gcgataagtt tccgcgctat
      181 ttgtgatacc taattattaa gcatggtcaa aattatgcga aactaaaaaa aaaaaacgtg
      241 agtaagccgt aaagatgagg cctaatcggg taaccaattc cctgcaacgt tcagcgcgag
      301 aaacaaagaa gttcaatgaa actgatagaa cgaagaaaaa aaaaaaaaaa gattataaaa
      361 accaaatcaa ctccttcttc ttcttttcat tttctagtct taccattgat taattaactt
      421 acttacttat tttatcaatt gttactataa taatgtctga atcaactgtt ggaaaagtat
      481 gtaactcaat tcatttatcc cccttttttt tccatttgtt ctttcttaat tgaaattgga
      541 aaatgatttt caattggtgg agaaaattgt taattttttt ttttttttga aaaaaaggga
      601 aattttcatc atattttata cccacacacc atgagcaatt cgaaaatcac cctagtggtt
      661 aaaacataaa ctcaaaaaaa agaaattatg ttcattttac taactttact cagccaatta
      721 cctgtaaagc cgctgttgct tgggaagctg ctaaaccatt atccattgaa gatgtcactg
      781 ttgctccacc aaaacgtcat gaagtccgta tcaaacttta tgacactggt gtttgtcaca
      841 ctgatgctta cactttaagt ggggttgatc cagaaggtgc tttcccagtt attttgggac
      901 atgaaggtgc cggtattgtc gaaagtattg gtgaaggtgt caccaatgtt aaagttggtg
      961 accatgtcat tgccttgtat accccagaat gtggtgaatg taaattctgt aaatctggta
     1021 aaaccaactt gtgtggtaaa attagagcta cccaaggtaa aggtgttatg ccagatggta
     1081 cttcaagatt tacatgcaaa ggtaaagaaa ttctccactt tatgggttgt tccactttct
     1141 cccaatatac tgttgttgct gacatttctg ttgttgccat caatccaaaa gctgaatttg
     1201 acaaagcttg tttgttaggt tgtggtatta ctactggtta tggtgccgcc accatcaccg
     1261 ccaacgttca aaaaggtgac aacgttgctg ttttcggtgg tggtattgtc gggttatccg
     1321 tcattcaagg ttgtgctgaa agaggagctg ctcaaatcat tttagttgat atcagtgaca
     1381 agaaagaaga atggggtcaa aaacttggtg ccactgcctt tgttaaccca accaaattac
     1441 cagaaggtac cactattgtc gataaattga ttgaaatgac cgatggtggt tgtgatttca
     1501 cttttgattg taccggtaat gttggggtta tgagaaacgc tttggaagct tgtcataaag
     1561 gttggggtac ttccgtcatt attggtgttg ctgcagctgg taaagaaatt tccaccagac
     1621 cattccaatt ggttaccggt agaacttgga aaggagctgc tttcggtggt gttaaaggta
     1681 gatcccaatt accaggaatt gttaacaatt acttggatgg taaattgaag gttgaagaat
     1741 tcattaccca cagagaacct ttggcagcca ttaacaaagc ttttgaagaa atgcatgctg
     1801 gtgattgtat cagagctgtt gttgacttgt catgactaga cagcaaactt taagttgttt
     1861 gtttgttcgt ttgtaattaa gatacgtata tattatctat tattataacc ttcaaataaa
     1921 aataaaaata aaaaatggat tactaaaaat tctattcatt atctgaatct gtcactaatt
     1981 ctgcagctga tggaatatct acatcttcat cgtcttcgtc ttcgtcttca tcgtcttctt
     2041 cactttcttc atagtcatcg acaccatcca tgatttcatc ttcatcatcg tcgctgataa
     2101 caccaatata ctgagcatcg tcaagttctt cgatatattc cacgtcactt ccttcttcca
     2161 catattcatc ttcaacatca tcttcaacga aaatttcatt cttcaaatca gtattatcat
     2221 taagtaaatt caatctacct tgtaattcca ataacctt
//