From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human immunodeficiency virus type 1, isolate BRU, complete genome
Date: 10 Sep 92 10:32:02 GMT
Distribution: bionet
Checksum: 17593    21

LOCUS       HIVBRUCG     9229 bp ss-RNA             VRL       10-SEP-1992
DEFINITION  Human immunodeficiency virus type 1, isolate BRU, complete genome
            (LAV-1).
ACCESSION   K02013
KEYWORDS    TAR protein; TAT protein; acquired immune deficiency syndrome;
            complete genome; env protein; gag protein;
            long terminal repeat (LTR); pol protein; polyprotein;
            proviral gene; reverse transcriptase; trans-activator.
SOURCE      Human immunodeficiency virus type 1 (HIV-1), isolate BRU (LAV-1),
            proviral DNA clone lambda-J19.
  ORGANISM  Human immunodeficiency virus type 1
            Viridae; ss-RNA enveloped viruses; Positive strand RNA virus;
            Retroviridae; Lentivirinae.
REFERENCE   1  (bases 1 to 9229)
  AUTHORS   Wain-Hobson,S., Sonigo,P., Danos,O., Cole,S. and Alizon,M.
  TITLE     Nucleotide sequence of the AIDS Virus, LAV
  JOURNAL   Cell 40, 9-17 (1985)
  STANDARD  full staff_review
REFERENCE   2  (bases 1 to 9193)
  AUTHORS   Van Beveren,C., Coffin,J. and Hughes,S.
  TITLE     Appendix B: HTLV-3/LAV genome
  JOURNAL   (in) Weiss,R., Teich,N., Varmus,H. and Coffin,J. (Eds.);
            RNA Tumor Viruses, Molecular Biology of Tumor Viruses, second
            edition, 2 : Supplements and Appendixes:  1106-1123,
            Cold Spring Harbor Laboratory, CSH, NY (1985)
  STANDARD  full staff_review
REFERENCE   3  (bases 1712 to 1749)
  AUTHORS   Alizon,M., Wain-Hobson,S., Gluckman,J.-C. and Sonigo,P.
  TITLE     Genetic variability of the AIDS virus: Nucleotide sequence analysis
            of two isolates from African patients
  JOURNAL   Cell 46, 63-74 (1986)
  STANDARD  full staff_review
COMMENT
            [(in) Weiss,R., Teich,N., Varmus,H. and Coffin,J. (Eds.);RNA Tumor
            Viruses,Molecu]  review.
            [3]  revision of [1].
            The original LAV, sometimes called LAV-1 to distinguish it from
            HIV2 (LAV-2), is now referred to as HIV-1bru. An infectious clone
            of this virus has been constructed by Keith Peden, Molecular Bio-
            logy and Genetics, Johns Hopkins University School of Medicine,
            Baltimore, MD 21205 (301) 955-3652. HIVNL43 is also an infectious
            clone having for its 3' half a clone of the BRU isolate.
            Acquired immune deficiency syndrome (AIDS) is caused by a
            retrovirus known by several different names, probably representing
            two separate strains: human T-cell lymphotropic virus-III
            (HTLV-III) and lymphadenopathy-associated virus (LAV) are thought
            to be one strain, and AIDS-associated retrovirus type 2 (ARV-2) the
            other.  All three viruses, whose sequences do not differ by more
            than about 6%, are believed to belong to the retroviral subfamily
            Lentiviridae, or 'slow' viruses.
            For the details of the annotation and for other pertinent
            references, see the HIV reference entry.
FEATURES             Location/Qualifiers
     LTR             <1..180
                     /note="5' LTR"
     repeat_region   <1..97
                     /note="R repeat 5' copy"
     exon            <5412..5626
                     /number=2
                     /note="tat protein,  (first expressed exon)"
     exon            <5551..5626
                     /number=2
                     /note="rev protein,  (first expressed exon)"
     CDS             join(5412..5626,7972..8017)
                     /note="tat protein"
                     /codon_start=1
                     /translation="MEPVDPRLEPWKHPGSQPKTACTTCYCKKCCFHCQVCFTTKALG
                     ISYGRKKRRQRRRPPQGSQTHQVSLSKQPTSQPRGDPTGPKE"
     CDS             join(5551..5626,7972..8246)
                     /note="rev protein"
                     /codon_start=1
                     /translation="MAGRSGDSDEDLLKAVRLIKFLYQSNPPPNPEGTRQARRNRRRR
                     WRERQRQIHSISERILSTYLGRSAEPVPLQLPPLERLTLDCNEDCGTSGTQGVGSPQI
                     LVESPTVLESGTKE"
     prim_transcript 1..9229
                     /note="genomic mRNA"
     prim_transcript 1..9229
                     /note="tat, rev, nef subgenomic mRNA"
     misc_binding    182..199
                     /bound_moiety="Lys"
                     /note="primer (Lys-tRNA) binding site"
     intron          290..5358
                     /note="tat, rev, nef subgenomic mRNA intron 1"
     CDS             336..1874
                     /note="gag polyprotein"
                     /codon_start=1
                     /translation="MGARASVLSGGELDRWEKIRLRPGGKKKYKLKHIVWASRELERF
                     AVNPGLLETSEGCRQILGQLQPSLQTGSEELRSLYNTVATLYCVHQRIEIKDTKEALD
                     KIEEEQNKSKKKAQQAAADTGHSSQVSQNYPIVQNIQGQMVHQAISPRTLNAWVKVVE
                     EKAFSPEVIPMFSALSEGATPQDLNTMLNTVGGHQAAMQMLKETINEEAAEWDRVHPV
                     HAGPIAPGQMREPRGSDIAGTTSTLQEQIGWMTNNPPIPVGEIYKRWIILGLNKIVRM
                     YSPTSILDIRQGPKEPFRDYVDRFYKTLRAEQASQEVKNWMTETLLVQNANPDCKTIL
                     KALGPAATLEEMMTACQGVGGPGHKARVLAEAMSQVTNSATIMMQRGNFRNQRKIVKC
                     FNCGKEGHIARNCRAPRKKGCWKCGKEGHQMKDCTERQANFLGKIWPSYKGRPGNFLQ
                     SRPEPTAPPFLQSRPEPTAPPEESFRSGVETTTPSQKQEPIDKELYPLTSLRSLFGND
                     PSSQ"
     misc_feature    1631..4678
                     /note="ORF pol polypeptide bordered by stop codons"
                     /product="pol polyprotein"
                     /gene="pol"
                     /partial
     old_sequence    replace((1712.1749)..(1712.1749)
                     /citation=[1]
     CDS             4623..5201
                     /note="vif protein"
                     /codon_start=1
                     /translation="MENRWQVMIVWQVDRMRIRTWKSLVKHHMYVSGKARGWFYRHHY
                     ESPHPRISSEVHIPLGDARLVITTYWGLHTGERDWHLGQGVSIEWRKKRYSTQVDPEL
                     ADQLIHLYYFDCFSDSAIRKALLGHIVSPRCEYQAGHNKVGSLQYLALAALITPKKIK
                     PPLPSVTKLTEDRWNKPQKTKGHRGSHTMNGH"
     CDS             5141..5431
                     /note="vpr protein"
                     /codon_start=1
                     /translation="MEQAPEDQGPQREPHNEWTLELLEELKNEAVRHFPRIWLHGLGQ
                     HIYETYGDTWAGVEAIIRILQQLLFIHFRIGCRHSRIGVTQQRRARNGASRS"
     intron          5627..7971
                     /note="tat cds intron 2"
     intron          5627..7971
                     /note="rev cds intron 2"
     intron          5627..7971
                     /note="tat, rev, nef subgenomic mRNA intron 2"
     CDS             5643..5888
                     /note="vpu protein"
                     /codon_start=1
                     /translation="MQPIQIAIAALVVAIIIAIVVWSIVIIEYRKILRQRKIDRLIDR
                     LIERAEDSGNESEGEISALVEMGVEMGHHAPWDIDDL"
     CDS             5803..8388
                     /note="envelope polyprotein"
                     /codon_start=1
                     /translation="MRVKEKYQHLWRWGWKWGTMLLGILMICSATEKLWVTVYYGVPV
                     WKEATTTLFCASDAKAYDTEVHNVWATHACVPTDPNPQEVVLVNVTENFNMWKNDMVE
                     QMHEDIISLWDQSLKPCVKLTPLCVSLKCTDLGNATNTNSSNTNSSSGEMMMEKGEIK
                     NCSFNISTSIRGKVQKEYAFFYKLDIIPIDNDTTSYTLTSCNTSVITQACPKVSFEPI
                     PIHYCAPAGFAILKCNNKTFNGTGPCTNVSTVQCTHGIRPVVSTQLLLNGSLAEEEVV
                     IRSANFTDNAKTIIVQLNQSVEINCTRPNNNTRKSIRIQRGPGRAFVTIGKIGNMRQA
                     HCNISRAKWNATLKQIASKLREQFGNNKTIIFKQSSGGDPEIVTHSFNCGGEFFYCNS
                     TQLFNSTWFNSTWSTEGSNNTEGSDTITLPCRIKQFINMWQEVGKAMYAPPISGQIRC
                     SSNITGLLLTRDGGNNNNGSEIFRPGGGDMRDNWRSELYKYKVVKIEPLGVAPTKAKR
                     RVVQREKRAVGIGALFLGFLGAAGSTMGARSMTLTVQARQLLSGIVQQQNNLLRAIEA
                     QQHLLQLTVWGIKQLQARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSL
                     EQIWNNMTWMEWDREINNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITN
                     WLWYIKIFIMIVGGLVGLRIVFAVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEE
                     EGGERDRDRSIRLVNGSLALIWDDLRSLCLFSYHRLRDLLLIVTRIVELLGRRGWEAL
                     KYWWNLLQYWSQELKNSAVSLLNATAIAVAEGTDRVIEVVQGACRAIRHIPRRIRQGL
                     ERILL"
     exon            7972..>8017
                     /number=3
                     /note="tat protein"
     exon            7972..>8246
                     /number=3
                     /note="rev protein"
     CDS             8390..9010
                     /note="nef protein"
                     /codon_start=1
                     /translation="MGGKWSKSSVVGWPTVRERMRRAEPAADGVGAASRDLEKHGAIT
                     SSNTAATNAACAWLEAQEEEEVGFPVTPQVPLRPMTYKAAVDLSHFLKEKGGLEGLIH
                     SQRRQDILDLWIYHTQGYFPDWQNYTPGPGVRYPLTFGWCYKLVPVEPDKVEEANKGE
                     NTSLLHPVSLHGMDDPEREVLEWRFDSRLAFHHVARELHPEYFKNC"
     LTR             8679..>9229
                     /note="3' LTR"
     repeat_region   9133..9229
                     /note="R repeat 3' copy"
     polyA_signal    9205..9210
                     /note="mRNA polyadenylation signal"
BASE COUNT     3289 a   1656 c   2232 g   2052 t
ORIGIN      Cap site of genomic RNA.
        1 ggtctctctg gttagaccag atttgagcct gggagctctc tggctaacta gggaacccac
       61 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
      121 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
      181 gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag
      241 gactcggctt gctgaagcgc gcacggcaag aggcgagggg aggcgactgg tgagtacgcc
      301 aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa
      361 gcgggggaga attagatcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
      421 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
      481 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
      541 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
      601 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
      661 acaaaagtaa gaaaaaagca cagcaagcag cagctgacac aggacacagc agccaggtca
      721 gccaaaatta ccctatagtg cagaacatcc aggggcaaat ggtacatcag gccatatcac
      781 ctagaacttt aaatgcatgg gtaaaagtag tagaagagaa ggctttcagc ccagaagtga
      841 tacccatgtt ttcagcatta tcagaaggag ccaccccaca agatttaaac accatgctaa
      901 acacagtggg gggacatcaa gcagccatgc aaatgttaaa agagaccatc aatgaggaag
      961 ctgcagaatg ggatagagtg catccagtgc atgcagggcc tattgcacca ggccagatga
     1021 gagaaccaag gggaagtgac atagcaggaa ctactagtac ccttcaggaa caaataggat
     1081 ggatgacaaa taatccacct atcccagtag gagaaattta taaaagatgg ataatcctgg
     1141 gattaaataa aatagtaaga atgtatagcc ctaccagcat tctggacata agacaaggac
     1201 caaaagaacc ctttagagac tatgtagacc ggttctataa aactctaaga gccgagcaag
     1261 cttcacagga ggtaaaaaat tggatgacag aaaccttgtt ggtccaaaat gcgaacccag
     1321 attgtaagac tattttaaaa gcattgggac cagcagctac actagaagaa atgatgacag
     1381 catgtcaggg agtgggagga cccggccata aggcaagagt tttggctgaa gcaatgagcc
     1441 aagtaacaaa ttcagctacc ataatgatgc aaagaggcaa ttttaggaac caaagaaaga
     1501 ttgttaagtg tttcaattgt ggcaaagaag ggcacatagc cagaaattgc agggccccta
     1561 ggaaaaaggg ctgttggaaa tgtggaaagg aaggacacca aatgaaagat tgtactgaga
     1621 gacaggctaa ttttttaggg aagatctggc cttcctacaa gggaaggcca gggaattttc
     1681 ttcagagcag accagagcca acagccccac catttcttca gagcagacca gagccaacag
     1741 ccccaccaga agagagcttc aggtctgggg tagagacaac aactccctct cagaagcagg
     1801 agccgataga caaggaactg tatcctttaa cttccctcag atcactcttt ggcaacgacc
     1861 cctcgtcaca ataaagatag gggggcaact aaaggaagct ctattagata caggagcaga
     1921 tgatacagta ttagaagaaa tgagtttgcc aggaagatgg aaaccaaaaa tgataggggg
     1981 aattggaggt tttatcaaag taagacagta tgatcagata ctcatagaaa tctgtggaca
     2041 taaagctata ggtacagtat tagtaggacc tacacctgtc aacataattg gaagaaatct
     2101 gttgactcag attggttgca ctttaaattt tcccattagt cctattgaaa ctgtaccagt
     2161 aaaattaaag ccaggaatgg atggcccaaa agttaaacaa tggccattga cagaagaaaa
     2221 aataaaagca ttagtagaaa tttgtacaga aatggaaaag gaagggaaaa tttcaaaaat
     2281 tgggcctgaa aatccataca atactccagt atttgccata aagaaaaaag acagtactaa
     2341 atggagaaaa ttagtagatt tcagagaact taataagaga actcaagact tctgggaagt
     2401 tcaattagga ataccacatc ccgcagggtt aaaaaagaaa aaatcagtaa cagtactgga
     2461 tgtgggtgat gcatattttt cagttccctt agatgaagac ttcaggaagt atactgcatt
     2521 taccatacct agtataaaca atgagacacc agggattaga tatcagtaca atgtgcttcc
     2581 acagggatgg aaaggatcac cagcaatatt ccaaagtagc atgacaaaaa tcttagagcc
     2641 ttttagaaaa caaaatccag acatagttat ctatcaatac atggatgatt tgtatgtagg
     2701 atctgactta gaaatagggc agcatagaac aaaaatagag gagctgagac aacatctgtt
     2761 gaggtgggga cttaccacac cagacaaaaa acatcagaaa gaacctccat tcctttggat
     2821 gggttatgaa ctccatcctg ataaatggac agtacagcct atagtgctgc cagaaaaaga
     2881 cagctggact gtcaatgaca tacagaagtt agtgggaaaa ttgaattggg caagtcagat
     2941 ttacccaggg attaaagtaa ggcaattatg taaactcctt agaggaacca aagcactaac
     3001 agaagtaata ccactaacag aagaagcaga gctagaactg gcagaaaaca gagagattct
     3061 aaaagaacca gtacatggag tgtattatga cccatcaaaa gacttaatag cagaaataca
     3121 gaagcagggg caaggccaat ggacatatca aatttatcaa gagccattta aaaatctgaa
     3181 aacaggaaaa tatgcaagaa cgaggggtgc ccacactaat gatgtaaaac aattaacaga
     3241 ggcagtgcaa aaaataacca cagaaagcat agtaatatgg ggaaagactc ctaaatttaa
     3301 actacccata caaaaggaaa catgggaaac atggtggaca gagtattggc aagccacctg
     3361 gattcctgag tgggagtttg tcaatacccc tcctttagtg aaattatggt accagttaga
     3421 gaaagaaccc atagtaggag cagaaacgtt ctatgtagat ggggcagcta gcagggagac
     3481 taaattagga aaagcaggat atgttactaa tagaggaaga caaaaagttg tcaccctaac
     3541 tgacacaaca aatcagaaga ctgagttaca agcaattcat ctagctttgc aggattcggg
     3601 attagaagta aatatagtaa cagactcaca atatgcatta ggaatcattc aagcacaacc
     3661 agataaaagt gaatcagagt tagtcaatca aataatagag cagttaataa aaaaggaaaa
     3721 ggtctatctg gcatgggtac cagcacacaa aggaattgga ggaaatgaac aagtagataa
     3781 attagtcagt gctggaatca ggaaagtact atttttagat ggaatagata aggcccaaga
     3841 tgaacatgag aaatatcaca gtaattggag agcaatggct agtgatttta acctgccacc
     3901 tgtagtagca aaagaaatag tagccagctg tgataaatgt cagctaaaag gagaagccat
     3961 gcatggacaa gtagactgta gtccaggaat atggcaacta gattgtacac atttagaagg
     4021 aaaagttatc ctggtagcag ttcatgtagc cagtggatat atagaagcag aagttattcc
     4081 agcagaaaca gggcaggaaa cagcatactt tcttttaaaa ttagcaggaa gatggccagt
     4141 aaaaacaata catacagaca atggcagcaa tttcaccagt actacggtta aggccgcctg
     4201 ttggtgggcg ggaatcaagc aggaatttgg aattccctac aatccccaaa gtcaaggagt
     4261 agtagaatct atgaataaag aattaaagaa aattataggc caggtaagag atcaggctga
     4321 acatcttaag acagcagtac aaatggcagt attcatccac aattttaaaa gaaaaggggg
     4381 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     4441 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
     4501 cagagatcca ctttggaaag gaccagcaaa gctcctctgg aaaggtgaag gggcagtagt
     4561 aatacaagat aatagtgaca taaaagtagt gccaagaaga aaagcaaaga tcattaggga
     4621 ttatggaaaa cagatggcag gtgatgattg tgtggcaagt agacaggatg aggattagaa
     4681 catggaaaag tttagtaaaa caccatatgt atgtttcagg gaaagctagg ggatggtttt
     4741 atagacatca ctatgaaagc cctcatccaa gaataagttc agaagtacac atcccactag
     4801 gggatgctag attggtaata acaacatatt ggggtctgca tacaggagaa agagactggc
     4861 atctgggtca gggagtctcc atagaatgga ggaaaaagag atatagcaca caagtagacc
     4921 ctgaactagc agaccaacta attcatctgt attactttga ctgtttttca gactctgcta
     4981 taagaaaggc cttattagga catatagtta gccctaggtg tgaatatcaa gcaggacata
     5041 acaaggtagg atctctacaa tacttggcac tagcagcatt aataacacca aaaaagataa
     5101 agccaccttt gcctagtgtt acgaaactga cagaggatag atggaacaag ccccagaaga
     5161 ccaagggcca cagagggagc cacacaatga atggacacta gagcttttag aggagcttaa
     5221 gaatgaagct gttagacatt ttcctaggat ttggctccat ggcttagggc aacatatcta
     5281 tgaaacttat ggggatactt gggcaggagt ggaagccata ataagaattc tgcaacaact
     5341 gctgtttatc catttcagaa ttgggtgtcg acatagcaga ataggcgtta ctcaacagag
     5401 gagagcaaga aatggagcca gtagatccta gactagagcc ctggaagcat ccaggaagtc
     5461 agcctaaaac tgcttgtacc acttgctatt gtaaaaagtg ttgctttcat tgccaagttt
     5521 gtttcacaac aaaagcctta ggcatctcct atggcaggaa gaagcggaga cagcgacgaa
     5581 gacctcctca aggcagtcag actcatcaag tttctctatc aaagcagtaa gtagtacatg
     5641 taatgcaacc tatacaaata gcaatagcag cattagtagt agcaataata atagcaatag
     5701 ttgtgtggtc catagtaatc atagaatata ggaaaatatt aagacaaaga aaaatagaca
     5761 ggttaattga tagactaata gaaagagcag aagacagtgg caatgagagt gaaggagaaa
     5821 tatcagcact tgtggagatg ggggtggaaa tggggcacca tgctccttgg gatattgatg
     5881 atctgtagtg ctacagaaaa attgtgggtc acagtctatt atggggtacc tgtgtggaag
     5941 gaagcaacca ccactctatt ttgtgcatca gatgctaaag catatgatac agaggtacat
     6001 aatgtttggg ccacacatgc ctgtgtaccc acagacccca acccacaaga agtagtattg
     6061 gtaaatgtga cagaaaattt taacatgtgg aaaaatgaca tggtagaaca gatgcatgag
     6121 gatataatca gtttatggga tcaaagccta aagccatgtg taaaattaac cccactctgt
     6181 gttagtttaa agtgcactga tttggggaat gctactaata ccaatagtag taataccaat
     6241 agtagtagcg gggaaatgat gatggagaaa ggagagataa aaaactgctc tttcaatatc
     6301 agcacaagca taagaggtaa ggtgcagaaa gaatatgcat ttttttataa acttgatata
     6361 ataccaatag ataatgatac taccagctat acgttgacaa gttgtaacac ctcagtcatt
     6421 acacaggcct gtccaaaggt atcctttgag ccaattccca tacattattg tgccccggct
     6481 ggttttgcga ttctaaaatg taataataag acgttcaatg gaacaggacc atgtacaaat
     6541 gtcagcacag tacaatgtac acatggaatt aggccagtag tatcaactca actgctgttg
     6601 aatggcagtc tagcagaaga agaggtagta attagatctg ccaatttcac agacaatgct
     6661 aaaaccataa tagtacagct gaaccaatct gtagaaatta attgtacaag acccaacaac
     6721 aatacaagaa aaagtatccg tatccagagg ggaccaggga gagcatttgt tacaatagga
     6781 aaaataggaa atatgagaca agcacattgt aacattagta gagcaaaatg gaatgccact
     6841 ttaaaacaga tagctagcaa attaagagaa caatttggaa ataataaaac aataatcttt
     6901 aagcaatcct caggagggga cccagaaatt gtaacgcaca gttttaattg tggaggggaa
     6961 tttttctact gtaattcaac acaactgttt aatagtactt ggtttaatag tacttggagt
     7021 actgaagggt caaataacac tgaaggaagt gacacaatca cactcccatg cagaataaaa
     7081 caatttataa acatgtggca ggaagtagga aaagcaatgt atgcccctcc catcagcgga
     7141 caaattagat gttcatcaaa tattacaggg ctgctattaa caagagatgg tggtaataac
     7201 aacaatgggt ccgagatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
     7261 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
     7321 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
     7381 ttcttgggag cagcaggaag cactatgggc gcacggtcaa tgacgctgac ggtacaggcc
     7441 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
     7501 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
     7561 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
     7621 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
     7681 atttggaata acatgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
     7741 atacattcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
     7801 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
     7861 ataaaaatat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
     7921 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
     7981 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
     8041 agagacagat ccattcgatt agtgaacgga tccttagcac ttatctggga cgatctgcgg
     8101 agcctgtgcc tcttcagcta ccaccgcttg agagacttac tcttgattgt aacgaggatt
     8161 gtggaacttc tgggacgcag ggggtgggaa gccctcaaat attggtggaa tctcctacag
     8221 tattggagtc aggaactaaa gaatagtgct gttagcttgc tcaatgccac agccatagca
     8281 gtagctgagg ggacagatag ggttatagaa gtagtacaag gagcttgtag agctattcgc
     8341 cacataccta gaagaataag acagggcttg gaaaggattt tgctataaga tgggtggcaa
     8401 gtggtcaaaa agtagtgtgg ttggatggcc tactgtaagg gaaagaatga gacgagctga
     8461 gccagcagca gatggggtgg gagcagcatc tcgagacctg gaaaaacatg gagcaatcac
     8521 aagtagcaat acagcagcta ccaatgctgc ttgtgcctgg ctagaagcac aagaggagga
     8581 ggaggtgggt tttccagtca cacctcaggt acctttaaga ccaatgactt acaaggcagc
     8641 tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca
     8701 acgaagacaa gatatccttg atctgtggat ctaccacaca caaggctact tccctgattg
     8761 gcagaactac acaccagggc caggggtcag atatccactg acctttggat ggtgctacaa
     8821 gctagtacca gttgagccag ataaggtaga agaggccaat aaaggagaga acaccagctt
     8881 gttacaccct gtgagcctgc atggaatgga tgaccctgag agagaagtgt tagagtggag
     8941 gtttgacagc cgcctagcat ttcatcacgt ggcccgagag ctgcatccgg agtacttcaa
     9001 gaactgctga catcgagctt gctacaaggg actttccgct ggggactttc cagggaggcg
     9061 tggcctgggc gggactgggg agtggcgagc cctcagatgc tgcatataag cagctgcttt
     9121 ttgcctgtac tgggtctctc tggttagacc agatttgagc ctgggagctc tctggctaac
     9181 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttca
//