From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: P.vulgaris genes glnA, ntrB and ntrC
Date: 11 Sep 92 10:06:39 GMT
Distribution: bionet
Checksum: 57691    10

LOCUS       PRMGLNABC    4362 bp ds-DNA             BCT       11-SEP-1992
DEFINITION  P.vulgaris genes glnA, ntrB and ntrC
ACCESSION   X68129
KEYWORDS    glnA gene; glutamate-ammonia ligase; nitrogen regulator; ntrB gene;
            ntrC gene; two-component regulatory system.
SOURCE      Proteus vulgaris DNA.
  ORGANISM  Proteus vulgaris
            Prokaryotae; Gram-negative facultatively anaerobic rods;
            Enterobacteriaceae.
REFERENCE   1  (bases 1 to 4362)
  AUTHORS   Steglitz-Moersdorf,U., Moersdorf,G. and Kaltwasser,H.
  TITLE     Cloning heterologous expression and sequencing of the proteus
            vulgaris glnAntrBC operon and implications of nitrogen control
            heterologous urase expression
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
COMMENT     *source: strain=DSM 30118;
            From EMBL    entry PVGLNABC;  dated 27-AUG-1992.
FEATURES             Location/Qualifiers
     protein_bind    37..51
                     /bound_moiety="NR I"
     protein_bind    71..85
                     /bound_moiety="NR I"
     promoter        160..175
                     /note="-24/-12 signal"
     CDS             259..1668
                     /EC_number="6.3.1.2"
                     /product="glutamate--ammonia ligase"
                     /gene="glnA"
                     /evidence=EXPERIMENTAL
                     /codon_start=1
                     /translation="MSLEHVLSMIEEHKVRYIDLRFTDTRGKEQHLTIPAHQVNDDFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAMLDPFFQDPTLIIRCDVLEPGTMQGYD
                     RDPRSISKRAEDYLKSSGIADTVLFGPEPEFFLFDDIRFKNDISGASYAINDIEAAWN
                     TNTKYEDGNKGHRPMVKGGYFPLPPVDSSQDIRSTMCNIMEEMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADETQIYKYVVQNVAHVFGKTATFMPKPLVGDNGSGMHCHMSLS
                     KNGTNLFAGDKYGGLSEMALYYIGGIIKHARALNAFTNPTTNSYKRLVPGFEAPVMLA
                     YSARNRSASIRIPVVASMKARRIEVRFPDPLANPYLAFAAQLMAGLDGITNKIHPGDA
                     MDKNLYDLPPEEAKEIPTVAGSLEEALNALDADREFLTHGGVFTNDSIDAYLALLRTD
                     VQRVRMAPHPLEFEMYYSA"
     -35_signal      1675..1707
     terminator      1735..1752
     protein_bind    1824..1838
                     /bound_moiety="NR I"
     CDS             1854..2900
                     /product="NR II"
                     /gene="ntrB"
                     /codon_start=1
                     /translation="METGNLPDNTLILDSLIHSVLVINQEFIICYANHAALQVLAQSR
                     RKLFETPFTDLFSYHSFDAELMQETLANGQSFTDNEVILVIHNQSHTMSLSAQPISEQ
                     HILLELAPMDSQRRLSQEQIQQAQQIAARELVRGLAHEIKNPLGGLRGAAQLLAKSLP
                     DPALTEYTQVIIEQADRLRTLVDRLLGPQHPGKKTHQSIHHVVERVAQLISLECPENV
                     TLLKDYDPSLPELSHYPDQIEQVLLNITRNALQAVEKTGGTIILRTRTAFQITLHGER
                     HRLVARIDVIDTGSGIPPHLQDTLFYPMVSGREDGNGLGLSIARNLVDQHAGKIEFTS
                     WPGNTEFSIYLPIK"
     CDS             2911..4332
                     /product="NR I"
                     /gene="ntrC"
                     /evidence=EXPERIMENTAL
                     /codon_start=1
                     /translation="MQKGNVWVVDDDSSIRWVLERAITREGMLCRAFEHANDVLKALN
                     SEQPDVLLSDIRMPDMDGLSLLKIIKEQYPTLPVIIMTAHSDLDAAVNAYQQGAFDYL
                     PKPFDIDETLALIERAITHYREQKQPNNAENILQSVSDMIGEAPAMQEVYRIIGRLSR
                     SSISVLINGESGTGKELVAHALHRHSPRALAPFIALNMAAIPKDLIESELFGHEKGAF
                     TGASQVRQGRFEQANGGSLFLDEIGDMPLDIQTRLLRVLAEGQFYRVGGYAPVKVDVR
                     IIAATHQDLEKRVNEGDFREDLYHRLNVIRIQLPPLRDRTEDIPSLARYFLQKTAKEL
                     GVETKSLHEQSLKTMMEYVWSGNVRQLENVCRWLTVMTASQEIMPQDLPSEIRLADEK
                     AKNINRLTSQHWSQHLSLWADEALGEGKENILNDALPQFERTLLLSALAYTQGHKQDA
                     ARLLGWGRNTLTRKLKELGIEDY"
BASE COUNT     1331 a    909 c    886 g   1236 t
ORIGIN
        1 ttttcacgcc tcagaattta gtggcattac atgattgcac caaaatagtg cactcaatta
       61 ggcttaattt gcaccagtat tgtgcaataa ttaaaaaatc acatgaggta ttctacacta
      121 aacttcatga atacagtata tatccctttt tatataagtt ggcatggttt tcgcaatagt
      181 attttggtga gcaaaaaatt caccaaaaaa tgtgtaaggc cactgcacta aataaaacca
      241 ctcgccagga gagtttatat gtcccttgaa catgtattat ccatgattga agaacataaa
      301 gttagatata ttgacttacg ttttactgat acccgcggca aagaacaaca tctgactatt
      361 cctgcccatc aggttaacga tgatttcttt gaagaaggaa aaatgttcga tggatcttct
      421 attggcggtt ggaaaggcat taacgaatca gacatggtac tgatgcctga tgcttctaca
      481 gcaatgcttg acccattttt tcaagatcca acactgatta ttcgttgtga tgtattagaa
      541 ccaggcacaa tgcaaggtta tgatcgcgac cctcgttcaa tctcaaaacg agctgaagat
      601 tacctaaaat caagcggtat cgccgacact gttttatttg ggccagaacc tgaattcttc
      661 ttatttgatg atattcgttt taaaaatgat atttcaggcg cgtcatacgc tatcaatgac
      721 attgaagcag catggaacac taataccaaa tatgaagatg gtaacaaagg ccatcgtcct
      781 atggtaaaag ggggttattt cccattacca cccgttgatt catctcaaga tattcgctct
      841 accatgtgta acattatgga agagatggga ttagtggttg aagctcatca ccacgaagtc
      901 gcaacggcag gtcaaaacga agtcgcaaca cgttttaata ccatgaccaa aaaagcagat
      961 gaaacccaaa tttataaata cgtagtacaa aacgttgctc acgtatttgg caaaacggcg
     1021 acctttatgc caaaaccact cgtgggtgat aacggttcag gtatgcactg ccatatgtca
     1081 ctgtctaaaa atggaaccaa tctatttgcg ggtgacaaat acggcggatt atctgaaatg
     1141 gcgctgtact acattggcgg cattatcaaa catgctcgcg cattaaatgc gtttaccaac
     1201 ccgacaacca actcatacaa acgcttagtt ccgggttttg aagcgcctgt tatgctggct
     1261 tactctgcac gtaaccgctc agcgtctatt cgtatcccag ttgttgccag catgaaagcg
     1321 cgtcgtattg aagtacgttt cccagatcca ctcgcgaacc cttatcttgc ttttgctgct
     1381 cagttaatgg ccggtcttga tggcattacc aataaaattc acccaggtga tgctatggat
     1441 aaaaacctct atgacttacc gccagaagaa gcaaaagaga tcccaacagt agcgggttca
     1501 ttagaagaag cactgaatgc attagatgca gaccgtgaat tcttaactca tggtggtgta
     1561 ttcactaatg actcaattga tgcctattta gcgcttttac gtactgacgt tcagcgtgtt
     1621 cgtatggcac cacatcctct tgaatttgaa atgtattaca gcgcttaaac ttttttgcat
     1681 tacatttaat gcattgctgt agataatgat tttttgttgc cgtgaagcta ttttgcccat
     1741 cttcggatgg gcacttttct cccacttaag gagtcggtca ggttgcttac gcatctgttc
     1801 atttataaaa aaaaaggtaa gatgcactaa aatggtgcaa tggggtattc aatatggaaa
     1861 cgggcaactt acctgataac acactgattt tagactcttt aatccattca gtgctggtta
     1921 tcaatcaaga attcattatt tgttatgcaa accacgctgc gttacaagtt ttggcacaaa
     1981 gccgtcgcaa attatttgaa acacctttta ccgatttgtt tagttatcat tcatttgatg
     2041 ctgaacttat gcaagaaacc ttagcaaacg gacaaagttt cactgataac gaagtgattt
     2101 tggttattca taaccaatca cacactatgt cgctcagtgc ccaacctatt tccgagcaac
     2161 atattttgct ggaactggcg cctatggata gtcaacgtcg gttaagccaa gagcagatcc
     2221 aacaagccca gcaaatagct gcaagggaat tggttcgggg actggcacat gaaattaaaa
     2281 acccgctagg gggattgcga ggtgccgcac aattactggc aaaatcgtta cctgatcccg
     2341 cgctgacgga atatacacaa gtcattatcg aacaagcaga ccgtttgcgt acactggttg
     2401 atagactgtt aggccctcag caccctggta aaaaaacaca tcaaagcatt catcatgttg
     2461 ttgagcgtgt agcccaatta atttcattgg aatgcccaga aaatgtcaca ttattaaaag
     2521 attacgaccc aagcttacct gaactttctc attacccgga tcaaatagaa caagtcctac
     2581 tcaatattac gcgtaatgcc ttacaagcgg ttgaaaaaac aggcgggacg ataattttac
     2641 gtactcgcac cgcgtttcaa atcacgctac atggtgagcg tcacagactt gttgcacgca
     2701 tcgatgtgat agataccgga agcggtattc ccccacattt acaagatacc cttttctatc
     2761 caatggtaag cggaagagaa gacggaaatg gcttaggatt atctatcgct cgtaatttag
     2821 ttgatcagca cgcaggtaaa attgaattta ctagttggcc gggtaatacc gaattttcta
     2881 tttacctgcc aataaaatag gagataacca atgcaaaaag gaaatgtatg ggttgttgat
     2941 gatgacagct caattcgctg ggttcttgaa cgcgcaatta cacgagaagg tatgttatgt
     3001 cgtgcttttg aacatgctaa cgatgtactc aaggcgctaa atagcgaaca gcctgatgta
     3061 ttattatcag acattcgtat gcctgatatg gatggtttat ctctacttaa aataataaaa
     3121 gaacaatatc ctacactgcc cgttattatt atgactgcac attctgatct tgatgccgca
     3181 gtgaatgctt atcaacaagg tgcttttgat tacttaccaa aaccttttga tatcgatgaa
     3241 acgcttgcac taatagagag agcaatcaca cactatcgag agcaaaagca gccgaataat
     3301 gctgaaaata tacttcaatc tgtttctgat atgattggcg aagcccctgc aatgcaagaa
     3361 gtttatcgta ttatcggtcg actttctcgc tcatctatta gtgtgctaat taatggagaa
     3421 tcgggtacag gtaaagagct tgttgctcac gcactgcatc gtcatagccc tcgtgctttg
     3481 gctcctttta ttgccctaaa tatggcagcg atccccaaag atttaattga atctgaactc
     3541 tttggtcatg aaaaaggtgc gtttactggt gcttctcaag tacgccaagg ccgttttgaa
     3601 caagccaatg gtggttctct ttttctggat gaaattggtg atatgccatt agatatccaa
     3661 actcgtttat tacgcgtatt ggctgaaggg caattttatc gagttggagg ctatgcccct
     3721 gttaaagtgg atgtccgaat tattgcagca acccatcagg atttagaaaa acgagtgaat
     3781 gaaggcgatt ttcgtgaaga tctctaccat cgcctaaatg tgattcgtat ccaattacca
     3841 cctcttcgcg atagaacaga agatattcct agtttagcgc gctactttct gcaaaaaacg
     3901 gcaaaagagc tgggtgttga aaccaaatct ctgcatgaac aaagcttaaa aaccatgatg
     3961 gaatacgttt ggtctggaaa cgtaagacaa ctcgaaaatg tgtgtcgttg gctgacggtt
     4021 atgactgcaa gccaagaaat catgccacaa gatttaccgt ctgagatccg tttagcagat
     4081 gagaaagcaa aaaatattaa tcggcttaca tctcaacatt ggtcgcagca tctctcttta
     4141 tgggcagatg aagctttagg tgaaggcaaa gaaaatattc ttaatgatgc actgccacaa
     4201 tttgaacgca cgctattatt aagcgccctt gcttatacac aaggccataa acaagacgca
     4261 gcgcggctgt taggttgggg tagaaatacg ctaacacgca aacttaaaga attgggtata
     4321 gaggattatt aaccctccat accttaatga attagatccc cg
//