From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: E.coli rRNA operon (rrnB) coding for Glu-tRNA-2, 5S, 16S and 23S
Date: 15 Sep 92 10:02:58 GMT
Distribution: bionet
Checksum: 10149    14

LOCUS       ECORGNB      7508 bp ds-DNA             BCT       15-SEP-1992
DEFINITION  E.coli rRNA operon (rrnB) coding for Glu-tRNA-2, 5S, 16S and 23S
            rRNA.
ACCESSION   J01695
KEYWORDS    .
SOURCE      Escherichia coli DNA.
  ORGANISM  Escherichia coli
            Prokaryotae; Gram-negative facultatively anaerobic rods;
            Enterobacteriaceae.
REFERENCE   1  (bases 1518 to 3059)
  AUTHORS   Brosius,J., Palmer,M.L., Kennedy,P.J. and Noller,H.F.
  TITLE     Complete nucleotide sequence of a 16S ribosomal RNA gene from
            Escherichia coli
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 75, 4801-4805 (1978)
  STANDARD  full staff_review
REFERENCE   2  (bases 811 to 1543)
  AUTHORS   Csordas-Toth,E., Boros,I. and Venetianer,P.
  TITLE     Structure of the promoter region for the rrnB gene in Escherichia
            coli
  JOURNAL   Nucleic Acids Res. 7, 2189-2197 (1979)
  STANDARD  full staff_review
REFERENCE   3  (bases 3500 to 6403)
  AUTHORS   Brosius,J., Dull,T.J. and Noller,H.F.
  TITLE     Complete nucleotide sequence of a 23S ribosomal RNA gene from
            Escherichia coli
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 77, 201-204 (1980)
  STANDARD  full staff_review
REFERENCE   4  (bases 1 to 7508)
  AUTHORS   Brosius,J., Dull,T.J., Sleeter,D.D. and Noller,H.F.
  TITLE     Gene organization and primary structure of a ribosomal RNA operon
            from Escherichia coli
  JOURNAL   J. Mol. Biol. 148, 107-127 (1981)
  STANDARD  full staff_review
REFERENCE   5  (bases 6412 to 6507; 6603 to 6657)
  AUTHORS   Singh,B. and Apirion,D.
  TITLE     Primary and secondary structure in a precursor of 5S rRNA
  JOURNAL   Biochim. Biophys. Acta 698, 252-259 (1982)
  STANDARD  full staff_review
REFERENCE   6  (bases 6518 to 6931)
  AUTHORS   Sarmientos,P., Sylvester,J.E., Contente,S. and Cashel,M.
  TITLE     differential stringent control of the tandem e. coli ribosomal rna
            promoters from the rrna operon expressed in vivo in multicopy
            plasmids
  JOURNAL   Cell 32, 1337-1346 (1983)
  STANDARD  full staff_review
REFERENCE   7  (bases 3380 to 3509; 6392 to 6503)
  AUTHORS   King,T.C., Sirdeshmukh,R. and Schlessinger,D.
  TITLE     RNase III cleavage is obligate for maturation but not for function
            of Escherichia coli pre-23S rRNA
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 81, 185-188 (1984)
  STANDARD  full staff_review
REFERENCE   8  (sites)
  AUTHORS   Orosz,A., Boros,I. and Venetianer,P.
  TITLE     Analysis of the complex transcription termination region of the
            Escherichia coli rrnB gene
  JOURNAL   Eur. J. Biochem. 201, 653-659 (1991)
  STANDARD  full staff_review
REFERENCE   9  (sites)
  AUTHORS   Prestle,J., Schoenfelder,M., Adam,G. and Mundry,K.W.
  TITLE     Type 1 ribosome-inactivating proteins depurinate plant 25S rRNA
            without spexies specificity
  JOURNAL   Nucleic Acids Res. 20, 3179-3182 (1992)
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     misc_feature    1..250
                     /note="putative VECTOR sequence lambda"
                     /note="putative"
     conflict        replace(1068..1072
                     /citation=[2]
     misc_signal     1212..1218
                     /note="promoter: RNA polymerase binding site"
     misc_RNA        1223..>3306
                     /note="rrnB precursor RNA (alt.)"
     misc_signal     1331..1337
                     /note="alternate promoter"
     misc_RNA        1344..>3306
                     /note="rrnB precursor RNA (alt.)"
     conflict        replace(1507
                     /citation=[2]
     rRNA            1518..3059
                     /note="16S rRNA (rrnB)"
     tRNA            3231..3306
                     /anticodon=(pos:3265..3267,aa:Glu)
                     /note="Glu-tRNA-2 (rrnB)"
     rRNA            3500..6403
                     /note="23S rRNA (rrnB)"
     rRNA            6496..6615
                     /note="5S rRNA (rrnB)"
BASE COUNT     1969 a   1694 c   2166 g   1679 t
ORIGIN      1 bp upstream from BamHI site.
        1 ggatccgcct acctttcacg agttgcgcag tttgtctgca agactctatg agaagcagat
       61 aagcgataag tttgctcaac atcttctcgg gcataagtcg gacaccatgg catcacagta
      121 tcgtgatgac agaggcaggg agtgggacaa aattgaaatc aaataatgat tttattttga
      181 ctgatagtga cctgttcgtt gcaacaaatt gataagcaat gcttttttat aatgccaact
      241 tagtataaaa tagccaacct gttcgacaaa gattatgaga cagtctatgg ctaccaaact
      301 gcaggacggg aatacacctt gtctggcagc tacaccttct gaaccacgtc ccaccgtgct
      361 ggtgtttgac tccggcgtcg gtgggttgtc ggtctatgac gagatccggc atctcttacc
      421 ggatctccat tacatttatg ctttcgataa cgtcgctttc ccgtatggcg aaaaaagcga
      481 agcgtttatt gttgagcgag tggtggcaat tgtcaccgcg gtgcaagaac gttatcccct
      541 tgcgctggct gtggtcgctt gcaacactgc cagtaccgtt tcacttcctg cattacgcga
      601 aaagttcgac ttcccggttg ttggtgtcgt gccggcgatt aaacctgctg cacgtctgac
      661 ggcaaatggc attgtcggat tactggcaac ccgcggaaca gttaaacgtt cttatactca
      721 tgagctgatc gcgcgtttcg ctaatgaatg ccagatagaa atgctgggct cggcagagat
      781 ggttgagttg gctgaagcga agctacatgg cgaagatgtt tctctggatg cactaaaacg
      841 tatcctacgc ccgtggttaa gaatgaaaga gccgccagat accgttgtat tgggttgcac
      901 ccatttccct ctactacaag aagaactgtt acaagtgctg ccagagggaa cccggctggt
      961 ggattctggc gcagcgattg ctcgccgaac ggcctggttg ttagaacatg aagccccgga
     1021 tgcaaaatct gccgatgcga atattgcctt ttgtatggca atgacgccag gagctgaaca
     1081 attattgccc gttttacagc gttacggctt cgaaacgctc gaaaaactgg cagttttagg
     1141 ctgatttggt tgaatgttgc gcggtcagaa aattatttta aatttcctct tgtcaggccg
     1201 gaataactcc ctataatgcg ccaccactga cacggaacaa cggcaaacac gccgccgggt
     1261 cagcggggtt ctcctgagaa ctccggcaga gaaagcaaaa ataaatgctt gactctgtag
     1321 cgggaaggcg tattatgcac accccgcgcc gctgagaaaa agcgaagcgg cactgctctt
     1381 taacaattta tcagacaatc tgtgtgggca ctcgaagata cggattctta acgtcgcaag
     1441 acgaaaaatg aataccaagt ctcaagagtg aacacgtaat tcattacgaa gtttaattct
     1501 ttgagcgtca aacttttaaa ttgaagagtt tgatcatggc tcagattgaa cgctggcggc
     1561 aggcctaaca catgcaagtc gaacggtaac aggaagaagc ttgcttcttt gctgacgagt
     1621 ggcggacggg tgagtaatgt ctgggaaact gcctgatgga gggggataac tactggaaac
     1681 ggtagctaat accgcataac gtcgcaagac caaagagggg gaccttcggg cctcttgcca
     1741 tcggatgtgc ccagatggga ttagctagta ggtggggtaa cggctcacct aggcgacgat
     1801 ccctagctgg tctgagagga tgaccagcca cactggaact gagacacggt ccagactcct
     1861 acgggaggca gcagtgggga atattgcaca atgggcgcaa gcctgatgca gccatgccgc
     1921 gtgtatgaag aaggccttcg ggttgtaaag tactttcagc ggggaggaag ggagtaaagt
     1981 taataccttt gctcattgac gttacccgca gaagaagcac cggctaactc cgtgccagca
     2041 gccgcggtaa tacggagggt gcaagcgtta atcggaatta ctgggcgtaa agcgcacgca
     2101 ggcggtttgt taagtcagat gtgaaatccc cgggctcaac ctgggaactg catctgatac
     2161 tggcaagctt gagtctcgta gaggggggta gaattccagg tgtagcggtg aaatgcgtag
     2221 agatctggag gaataccggt ggcgaaggcg gccccctgga cgaagactga cgctcaggtg
     2281 cgaaagcgtg gggagcaaac aggattagat accctggtag tccacgccgt aaacgatgtc
     2341 gacttggagg ttgtgccctt gaggcgtggc ttccggagct aacgcgttaa gtcgaccgcc
     2401 tggggagtac ggccgcaagg ttaaaactca aatgaattga cgggggcccg cacaagcggt
     2461 ggagcatgtg gtttaattcg atgcaacgcg aagaacctta cctggtcttg acatccacgg
     2521 aagttttcag agatgagaat gtgccttcgg gaaccgtgag acaggtgctg catggctgtc
     2581 gtcagctcgt gttgtgaaat gttgggttaa gtcccgcaac gagcgcaacc cttatccttt
     2641 gttgccagcg gtccggccgg gaactcaaag gagactgcca gtgataaact ggaggaaggt
     2701 ggggatgacg tcaagtcatc atggccctta cgaccagggc tacacacgtg ctacaatggc
     2761 gcatacaaag agaagcgacc tcgcgagagc aagcggacct cataaagtgc gtcgtagtcc
     2821 ggattggagt ctgcaactcg actccatgaa gtcggaatcg ctagtaatcg tggatcagaa
     2881 tgccacggtg aatacgttcc cgggccttgt acacaccgcc cgtcacacca tgggagtggg
     2941 ttgcaaaaga agtaggtagc ttaaccttcg ggagggcgct taccactttg tgattcatga
     3001 ctggggtgaa gtcgtaacaa ggtaaccgta ggggaacctg cggttggatc acctccttac
     3061 cttaaagaag cgtactttgt agtgctcaca cagattgtct gatagaaagt gaaaagcaag
     3121 gcgtttacgc gttgggagtg aggctgaaga gaataaggcc gttcgctttc tattaatgaa
     3181 agctcaccct acacgaaaat atcacgcaac gcgtgataag caattttcgt gtccccttcg
     3241 tctagaggcc caggacaccg ccctttcacg gcggtaacag gggttcgaat cccctagggg
     3301 acgccacttg ctggtttgtg agtgaaagtc gccgacctta atatctcaaa actcatcttc
     3361 gggtgatgtt tgagattttt gctctttaaa aatctggatc aagctgaaaa ttgaaacact
     3421 gaacaacgag agttgttcgt gagtctctca aattttcgca acacgatgat gaatcgaaag
     3481 aaacatcttc gggttgtgag gttaagcgac taagcgtaca cggtggatgc cctggcagtc
     3541 agaggcgatg aaggacgtgc taatctgcga taagcgtcgg taaggtgata tgaaccgtta
     3601 taaccggcga tttccgaatg gggaaaccca gtgtgtttcg acacactatc attaactgaa
     3661 tccataggtt aatgaggcga accgggggaa ctgaaacatc taagtacccc gaggaaaaga
     3721 aatcaaccga gattccccca gtagcggcga gcgaacgggg agcagcccag agcctgaatc
     3781 agtgtgtgtg ttagtggaag cgtctggaaa ggcgcgcgat acagggtgac agccccgtac
     3841 acaaaaatgc acatgctgtg agctcgatga gtagggcggg acacgtggta tcctgtctga
     3901 atatgggggg accatcctcc aaggctaaat actcctgact gaccgatagt gaaccagtac
     3961 cgtgagggaa aggcgaaaag aaccccggcg aggggagtga aaaagaacct gaaaccgtgt
     4021 acgtacaagc agtgggagca cgcttaggcg tgtgactgcg taccttttgt ataatgggtc
     4081 agcgacttat attctgtagc aaggttaacc gaatagggga gccgaaggga aaccgagtct
     4141 taactgggcg ttaagttgca gggtatagac ccgaaacccg gtgatctagc catgggcagg
     4201 ttgaaggttg ggtaacacta actggaggac cgaaccgact aatgttgaaa aattagcgga
     4261 tgacttgtgg ctgggggtga aaggccaatc aaaccgggag atagctggtt ctccccgaaa
     4321 gctatttagg tagcgcctcg tgaattcatc tccgggggta gagcactgtt tcggcaaggg
     4381 ggtcatcccg acttaccaac ccgatgcaaa ctgcgaatac cggagaatgt tatcacggga
     4441 gacacacggc gggtgctaac gtccgtcgtg aagagggaaa caacccagac cgccagctaa
     4501 ggtcccaaag tcatggttaa gtgggaaacg atgtgggaag gcccagacag ccaggatgtt
     4561 ggcttagaag cagccatcat ttaaagaaag cgtaatagct cactggtcga gtcggcctgc
     4621 gcggaagatg taacggggct aaaccatgca ccgaagctgc ggcagcgacg cttatgcgtt
     4681 gttgggtagg ggagcgttct gtaagcctgc gaaggtgtgc tgtgaggcat gctggaggta
     4741 tcagaagtgc gaatgctgac ataagtaacg ataaagcggg tgaaaagccc gctcgccgga
     4801 agaccaaggg ttcctgtcca acgttaatcg gggcagggtg agtcgacccc taaggcgagg
     4861 ccgaaaggcg tagtcgatgg gaaacaggtt aatattcctg tacttggtgt tactgcgaag
     4921 gggggacgga gaaggctatg ttggccgggc gacggttgtc ccggtttaag cgtgtaggct
     4981 ggttttccag gcaaatccgg aaaatcaagg ctgaggcgtg atgacgaggc actacggtgc
     5041 tgaagcaaca aatgccctgc ttccaggaaa agcctctaag catcaggtaa catcaaatcg
     5101 taccccaaac cgacacaggt ggtcaggtag agaataccaa ggcgcttgag agaactcggg
     5161 tgaaggaact aggcaaaatg gtgccgtaac ttcgggagaa ggcacgctga tatgtaggtg
     5221 aggtccctcg cggatggagc tgaaatcagt cgaagatacc agctggctgc aactgtttat
     5281 taaaaacaca gcactgtgca aacacgaaag tggacgtata cggtgtgacg cctgcccggt
     5341 gccggaaggt taattgatgg ggttagcgca agcgaagctc ttgatcgaag ccccggtaaa
     5401 cggcggccgt aactataacg gtcctaaggt agcgaaattc cttgtcgggt aagttccgac
     5461 ctgcacgaat ggcgtaatga tggccaggct gtctccaccc gagactcagt gaaattgaac
     5521 tcgctgtgaa gatgcagtgt acccgcggca agacggaaag accccgtgaa cctttactat
     5581 agcttgacac tgaacattga gccttgatgt gtaggatagg tgggaggctt tgaagtgtgg
     5641 acgccagtct gcatggagcc gaccttgaaa taccaccctt taatgtttga tgttctaacg
     5701 ttgacccgta atccgggttg cggacagtgt ctggtgggta gtttgactgg ggcggtctcc
     5761 tcctaaagag taacggagga gcacgaaggt tggctaatcc tggtcggaca tcaggaggtt
     5821 agtgcaatgg cataagccag cttgactgcg agcgtgacgg cgcgagcagg tgcgaaagca
     5881 ggtcatagtg atccggtggt tctgaatgga agggccatcg ctcaacggat aaaaggtact
     5941 ccggggataa caggctgata ccgcccaaga gttcatatcg acggcggtgt ttggcacctc
     6001 gatgtcggct catcacatcc tggggctgaa gtaggtccca agggtatggc tgttcgccat
     6061 ttaaagtggt acgcgagctg ggtttagaac gtcgtgagac agttcggtcc ctatctgccg
     6121 tgggcgctgg agaactgagg ggggctgctc ctagtacgag aggaccggag tggacgcatc
     6181 actggtgttc gggttgtcat gccaatggca ctgcccggta gctaaatgcg gaagagataa
     6241 gtgctgaaag catctaagca cgaaacttgc cccgagatga gttctccctg accctttaag
     6301 ggtcctgaag gaacgttgaa gacgacgacg ttgataggcc gggtgtgtaa gcgcagcgat
     6361 gcgttgagct aaccggtact aatgaaccgt gaggcttaac cttacaacgc cgaagctgtt
     6421 ttggcggatg agagaagatt ttcagcctga tacagattaa atcagaacgc agaagcggtc
     6481 tgataaaaca gaatttgcct ggcggcagta gcgcggtggt cccacctgac cccatgccga
     6541 actcagaagt gaaacgccgt agcgccgatg gtagtgtggg gtctccccat gcgagagtag
     6601 ggaactgcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt
     6661 atctgttgtt tgtcggtgaa cgctctcctg agtaggacaa atccgccggg agcggatttg
     6721 aacgttgcga agcaacggcc cggagggtgg cgggcaggac gcccgccata aactgccagg
     6781 catcaaatta agcagaaggc catcctgacg gatggccttt ttgcgtttct acaaactctt
     6841 cctgtcgtca tatctacaag ccatcccccc acagatacgg taaactagcc tcgtttttgc
     6901 atcaggaaag cagctatgaa ccactcctta aaaccctgga acacatttgg cattgatcat
     6961 aatgctcagc acattgtatg tgccgaagac gaacaacaat tactcaatgc ctggcagtat
     7021 gcaaccgcag aaggacaacc cgttcttatt ctgggtgaag gaagtaatgt actttttctg
     7081 gaggactatc gcggcacggt gatcatcaac cggatcaaag gtatcgaaat tcatgatgaa
     7141 cctgatgcgt ggtatttaca tgtaggagcc ggagaaaact ggcatcgtct ggtaaaatac
     7201 actttgcagg aaggtatgcc tggtctggaa aatctggcat taattcctgg ttgtgtcggc
     7261 tcatcaccta tccagaatat tggtgcttat ggcgtagaat tacagcgagt ttgcgcttat
     7321 gttgattctg ttgaactggc gacaggcaag caagtgcgct taactgccaa agagtgccgt
     7381 tttggctatc gcgacagtat ttttaaacat gaataccagg accgcttcgc tattgtagcc
     7441 gtaggtctgc gtctgccaaa agagtggcaa cctgtactaa cgtatggtga cttaactcgt
     7501 ctggatcc
//