From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Sweet potato beta-amylase gene, complete cds.
Date: 9 Jan 93 05:46:55 GMT
Distribution: bionet
Checksum: 30270 9

LOCUS       IPBAMYB      4772 bp ds-DNA             PLN       09-JAN-1993
DEFINITION  Sweet potato beta-amylase gene, complete cds.
ACCESSION   D12882
KEYWORDS    beta-amylase.
SOURCE      Ipomoea batatas (strain Kokei No. 14) DNA.
  ORGANISM  Ipomoea batatas
            Eukaryota; Plantae; Embryobionta; Magnoliophyta; Magnoliopsida;
            Asteridae; Solanales; Convolvulaceae.
REFERENCE   1  (bases 1 to 4772)
  AUTHORS   Yoshida,N., Hayashi,K. and Nakamura,K.
  TITLE     A nuclear gene encoding beta-amylase of sweet potato
  JOURNAL   Gene 120, 255-259 (1992)
  STANDARD  full automatic
COMMENT     Submitted (12-AUG-1992) to DDBJ by: Kenzo Nakamura Lab. of Biochem
            School of Agriculture Nagoya University Furo-cho, Chikusa-ku Nagoya
            464-01 Japan Phone:  052-781-5111 x6313 Fax:    052-782-9162.
FEATURES             Location/Qualifiers
     TATA_signal     1236..1242
     exon            1268..1414
                     /number=1
     intron          1415..1608
                     /number=1
     exon            1609..2007
                     /number=2
     intron          2008..2161
                     /number=2
     exon            2162..2326
                     /number=3
     intron          2327..2414
                     /number=3
     exon            2415..2681
                     /number=4
     intron          2682..2762
                     /number=4
     exon            2763..2972
                     /number=5
     intron          2973..3726
                     /number=5
     exon            3727..3966
                     /number=6
     intron          3967..4086
                     /number=6
     exon            4087..4546
                     /number=7
     CDS             join(1352..1414,1609..2007,2162..2326,2415..2681,
                     2763..2972,3727..3966,4087..4242)
                     /EC_number="3.2.1.2"
                     /gene="beta-Amy"
                     /product="beta-amylase"
                     /codon_start=1
                     /translation="MAPIPGVMPIGNYVSLYVMLPLGVVNADNVFPDKEKVEDELKQV
                     KAGGCDGVMVDVWWGIIEAKGPKQYDWSAYRELFQLVKKCGLKIQAIMSFHQCGGNVG
                     DAVFIPIPQWILQIGDKNPDIFYTNRAGNRNQEYLSLGVDNQRLFQGRTALEMYRDFM
                     ESFRDNMADFLKAGDIVDIEVGCGAAGELRYPSYPETQGWVFPGIGEFQCYDKYMVAD
                     WKEAVKQAGNADWEMPGKGTGTYNDTPDKTEFFRPNGTYKTDMGKFFLTWYSNKLIIH
                     GDQVLEEANKVFVGLRVNIAAKVSGIHWWYNHVSHAAELTAGFYNVAGRDGYRPIARM
                     LARHHATLNFTCLEMRDSEQPAEAKSAPQELVQQVLSSGWKEYIDVAGENALPRYDAT
                     AYNQILLNVRPNGVNLNGPPKLKMSGLTYLRLSDDLLQTDNFELFKKFVKKMHADLDP
                     SPNAISPAVLERSNSAITIDELMEATKGSRPFPWYDVTDMPVDGSNPFD"
BASE COUNT     1480 a    861 c    978 g   1453 t
ORIGIN      
        1 gaattcccgt acaaagtatt aaacgtgcca cagtctaatg aaaagtctcc tacttatact
       61 agtgcattca acgttattct gctcaagtaa cgatccatag taatggagaa ccagatttac
      121 attaaagtgt gttattcatc ataagtcttc tcttgaatag atcggtatta gagtaaatat
      181 ttctattatg tgactgctgg ttttctttga atgattttgg atccggtgtc tctccaatta
      241 tcttgtcaat tacaaattta attatttatg aaagtgattt attgacataa tttaatcaat
      301 acatatcctt aaatagagat tacacgaagg tagctcttgc tgcaattatt aatgcacttt
      361 taaaggcgga ccatggtcca tgggtacaca gtagaaggat tgtcattttc ctgttatatc
      421 aatcattccc ttaatggacg gtattgggag ggcggatcgt cacgtcacgt ttttagactc
      481 cgcggcatgc atacacgtgg cgttacgttg gcgatttgac agcttggcct caattttact
      541 ttaactcaac aaaacacaaa cgtgctaaat gccaaccatg gtccacggat ttgcctttgg
      601 aatgggttag tgttaatttt ggtcgatact aatttcgtgt gtaatatata tattttttat
      661 tattaaaaat atatttatta ttaattatgt atatgattgt tgaaaaaagc gatatttgga
      721 tgtaataaaa cgcaaggaaa agtgagaatt aagaatgata acacttcatg catgaattta
      781 atacaatttt tttgtgtaat aattcatcct acatttaatt gttttgcaac actattagtt
      841 gttaacgctc tccttgcatt ttaatttcct gataaaaata catacaaaat gctaacatta
      901 gataaaacgt tttattttta attgtgtatt aatagataca taccatgcat acataattat
      961 tagtgcgatg cggtaacata tgaatatgat cgagtaagat ttgaataatt gagttcgtgt
     1021 actttagtga atttaatgaa gtatttttat atttagccat tttcaaactt caaggatcac
     1081 aaatcttagc ttggtgaaag acacatcaaa atttggatgc ttaataatta taactcattt
     1141 tccaaaaaaa aaaaattaaa aattataact catgtgcatg caatactatt ataatagtat
     1201 attttaacaa ctcatcacca taccaaacca ttgtgtatat aaagggatca tcaatgcaat
     1261 actaggctgt atagccactt ctcaatcttc tccattattc tcagctctct ctctctcact
     1321 cttgctttag tctcttgcaa aataaccaac aatggctcca atccccggtg taatgccaat
     1381 tggtaactac gtctctctgt atgtaatgct cccggtaagc caatattatc atcatattca
     1441 attctgtact taattagtgt caattgcccc gattagtgaa aaatatatga atttataaaa
     1501 ctatgatcga gttaaactga attcaaattt ttcgtgtaat ttggttcata tacattactg
     1561 tatcaatcga tctgagtaca cttattattt ttttgtgtgg cgggaaagtt gggagttgtg
     1621 aatgcggaca atgtttttcc ggacaaagag aaggtggaag atgagctgaa gcaggtgaaa
     1681 gcaggggggt gcgatggggt gatggtggat gtgtggtggg ggatcatcga ggccaaaggg
     1741 ccaaagcagt acgattggtc tgcttacagg gagttattcc agttggttaa gaaatgtggg
     1801 ctcaaaatcc aggcgatcat gtcttttcac caatgcggcg gcaatgtcgg cgacgccgtc
     1861 ttcatcccca tccctcaatg gattctccaa atcggcgaca aaaaccccga tatcttctac
     1921 accaaccggg ccggtaaccg aaaccaggag tacctctctc tcggcgtcga caatcaacgt
     1981 ctcttccagg gccgcaccgc tctcgaggta actctagtct tattatcagt ttaaactttt
     2041 ttgtagtcat tatcagaaag tgtatactga ttcagtaaat ctgttttgtg acttgataga
     2101 ttgattatca aattagggcg aaattgaact tattattgat atggttgaga tgtacttgta
     2161 gatgtacagg gatttcatgg aaagtttcag ggataatatg gcagactttt tgaaggctgg
     2221 agatattgta gacattgaag tagggtgtgg ggctgccgga gagcttcggt atccctcgta
     2281 tcccgagact caaggatggg tttttcccgg catcggagaa tttcaggtca gtcttgattt
     2341 aggctttaca agttacaacg taacaatggc ggatatgaga attgaatgat tatattacaa
     2401 gttttaattt gcagtgctat gacaagtaca tggtggcaga ctggaaggag gctgtgaagc
     2461 aagctgggaa tgcagattgg gagatgccgg gaaaaggcac cgggacttac aacgacacgc
     2521 cggacaagac ggaattcttc cgcccaaacg ggacttacaa gacggatatg ggcaagtttt
     2581 tcttgacctg gtactccaac aagctcatca tccatggcga tcaagtcctc gaagaagcca
     2641 acaaagtttt cgttgggctc cgcgtcaaca tagctgccaa agtacacctt taaattaatt
     2701 tatttctcca tctcaccctc ttaattaatt aaattactca tcagctttag ctgtatgcgc
     2761 aggtttctgg aattcactgg tggtacaacc atgtgagcca tgcggcggag ctcaccgccg
     2821 gattctacaa tgtggcggga agagacggtt atcggcctat tgccaggatg ctggcaaggc
     2881 accatgccac tctgaatttc acttgccttg agatgagaga ctccgaacag cctgccgagg
     2941 ccaagagtgc tcctcaagaa ctcgttcaac aggtacgtac cataaccaaa attctgcaac
     3001 atcaaaatga atatataaaa gaagcctagg agctagcata gtggttgatc acctacctca
     3061 agtcataact gaaatcggag gtaggggttg attgtgttag cctaatttga gcagtagtga
     3121 tgtagtgaat acatcgaaat atacgaatat gtctaagtat cgtctagggt tcggtatgca
     3181 attcagcaag agtaaatgtg cgggcttggg agctttatat agaggttgga ggggacacta
     3241 ccaaaaaaaa gtagtgccgc tttctttcat aacagagttg tggttcccgg cacatgtcgc
     3301 gcttgtcctt tagcataaaa ggatgtgtaa ttgaaataga cattaaggtc ggatgttata
     3361 gaggttggaa gggacacatg ccgagcttac cctttagcat acaagtatgt cggatcaaaa
     3421 taacccgtct agacattaag gtccgacgtt atagaggttg gaggggacac atgtcggact
     3481 tgtgtttagc atacaaggat gtccgatcaa aataacgggt caggcgttcc aactcaagtt
     3541 cagggtaaat tgtgcttcca ctaagactca aatgcacaac ctcccatatg aaagtacagt
     3601 ttagtgtcat aaaccacaag atcttagctt gtggtcttct ttatttaagc ccggccacta
     3661 gctatttacc aacttggtta ctttggacat gttaattaat taaagttgag ttgaatgatt
     3721 gtgcaggtgt tgagcagcgg atggaaagag tatatcgatg tggcaggtga gaatgcactg
     3781 ccaagatatg atgccacggc atacaaccaa atacttctga acgtcaggcc aaacggcgtc
     3841 aaccttaacg gccctcccaa gctcaagatg tccggcttga catatctccg tttgtcggac
     3901 gatctgttac agacagacaa cttcgaactc ttcaagaaat tcgtcaagaa gatgcacgct
     3961 gatctggtca ccattattat ttgtttaaat ctccaaacaa attaaaatta tttaatcaaa
     4021 agactaatta cattaattaa caatcttatg tagtatggaa taataagatt gttaaataat
     4081 tttcaggatc catccccgaa cgctatctct ccggcggtgt tggagagatc aaactcggcg
     4141 atcaccattg atgaactgat ggaagccacc aaaggtagca ggccattccc atggtatgac
     4201 gtcacagaca tgccggtgga tggctcaaac ccgtttgatt gattaggatg acatcaccct
     4261 cctccctgga ctactgcatg tggaacaata ataagttaat gcataaatat tataaataaa
     4321 tgaataatcc agctttcagt gctgaaggtg aaggaggagc agagaagata gtgtttggat
     4381 aatgttgagg aattaataaa aaatggactt gagatgttgt gcaagccatt cattagtacg
     4441 tacgtagctt tgtatatgaa ttatgaagct aagagcctaa gaaataagaa cgttgtctaa
     4501 taatcataat cataataata ataatatgtg aagtaacttg tgatacgatg acatcaatgt
     4561 tataaatatt actaataagt tagaacgtat ccacaaattg tcattttcta ttttaataat
     4621 ttaaatttca taatcattac atgttaattt aagaacacaa aattacctta tgacttagaa
     4681 aatgaaacgt aattttcgta cttaaataaa atatttatac taactcgtat acctgtgggt
     4741 ataatattta tttatattat atagtatgtt cg
//