From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Bacillus subtilis valyl tRNA synthetase (valS) gene, 3' end cds;
Date: 6 Nov 92 18:26:29 GMT
Distribution: bionet
Checksum: 63601 6

LOCUS       BACFOLC      1800 bp ds-DNA             BCT       02-NOV-1992
DEFINITION  Bacillus subtilis valyl tRNA synthetase (valS) gene, 3' end cds;
            folyl-polyglutamate synthetase (folC) gene, complete cds; comC
            gene, 5' end cds.
ACCESSION   L04520
KEYWORDS    comC gene; folC gene; folyl-polyglutamate synthetase;
            ribosome binding site; terminator; transfer RNA-Val synthetase;
            valS gene.
SOURCE      Bacillus subtilis (sub_strain PY79, strain W168) DNA.
  ORGANISM  Bacillus subtilis
            Prokaryotae; Firmicutes; Endospore-forming rods and cocci;
            Bacillaceae.
REFERENCE   1  (sites)
  AUTHORS   Mohan,S., Aghion,J., Guillen,N. and Dubnau,D.
  TITLE     Molecular cloning and characterization of comC, a late competence
            gene of Bacillus subtilis
  JOURNAL   J. Bacteriol. 171, 6043-6051 (1989)
  STANDARD  full automatic
REFERENCE   2  (bases 1 to 1800)
  AUTHORS   Margolis,P.S., Driks,A. and Losick,R.
  TITLE     Sporulation gene spoIIB from Bacillus subtilis
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     terminator      176..211
                     /gene="valS"
     RBS             215..225
                     /gene="folC"
     conflict        replace(932..934,"gc")
                     /note="insertion of nucleotide 933 compared to sequence of
                     M30805"
                     /citation=[1]
     terminator      1537..1563
                     /gene="folC"
                     /citation=[1]
     RBS             1648..1654
                     /gene="comC"
                     /citation=[1]
     CDS             <1..171
                     /evidence="EXPERIMENTAL"
                     /gene="valS"
                     /product="valyl tRNA synthetase"
                     /codon_start=1
                     /translation="EFDKLTKEVERVQKKLGNEGFMKKAPAHVIDEEREKEKDYVAKR
                     DAVQKRMAELKG"
     CDS             231..1523
                     /gene="folC"
                     /product="folyl-polyglutamate synthetase"
                     /codon_start=1
                     /translation="MFTAYQDARSWIHGRLKFGVKPGLGRMKQLMARLGHPEKKIRAF
                     HVAGTNGKGSTVAFIRSMLQEAGYTVGTFTSPYIITFNERISVNGIPISDEEWTALVN
                     QMKPHVEALDQTEYGQPTEFEIMTACAFLYFAEFHKVDFVIFETGLGGRFDSTNVVEP
                     LLTVITSIGHDHMNILGNTIEEIAGEKAGIIKEGIPIVTAVTQPEALQVIRHEAERHA
                     APFQSLHDACVIFNEEALPAGEQFSFKTEEKCYEDIRTSLIGTHQRQNAALSILAAEW
                     LNKENIAHISDEALRSGLVKAAWPGRLELVQEHPPVYLDGAHNEEGVEKLAETMKQRF
                     ANSRISVVFSALKDKPYQNMIKRLETIAHAIHFASFDFPRASLAKDLYDASEISNKSW
                     SEDPDDVIKFIESKKGSNEIVLITGSLYFISDIRKRLK"
     CDS             1663..>1800
                     /function="required for the development of genetic
                     competence"
                     /label=comC
                     /citation=[1]
                     /gene="comC"
                     /product="comC"
                     /codon_start=1
                     /translation="MLSILFIFGLILGSFYYTAGCRIPLHLSIIAPRSSCPFCRRTLT
                     PA"
BASE COUNT      537 a    352 c    427 g    484 t
ORIGIN      
        1 gaatttgata aactgacaaa agaagttgag cgtgtccaaa agaaacttgg aaatgaagga
       61 tttatgaaaa aagcgcctgc acacgtaatt gatgaagaac gtgaaaaaga aaaagattat
      121 gtggcgaagc gtgacgctgt tcaaaaacga atggctgagc tgaagggtta atgaaaagcc
      181 ggggcggttt ctagagaaac cgccttgtct tataaaagag gggattatca ttgtttactg
      241 catatcaaga tgcgcgcagc tggattcacg ggaggctgaa attcggggtg aagcccggac
      301 ttggtcgaat gaaacagctg atggcgcggt taggacatcc tgaaaaaaaa atccgagcgt
      361 tccacgtcgc aggaacaaac ggtaaaggat caactgtcgc ttttatccgt tctatgctgc
      421 aggaagccgg atatacggtt ggaacattta catcgcctta tattattacg tttaatgaac
      481 ggatcagcgt aaacgggata ccgatttcag atgaggaatg gacagcactc gtcaatcaaa
      541 tgaaaccgca cgttgaagcc cttgatcaaa cggaatacgg acagccgaca gaatttgaaa
      601 ttatgacagc ttgtgcattt ttatattttg cagagtttca taaggtcgat tttgttattt
      661 ttgaaacagg tctgggcggt agattcgatt ctactaatgt ggttgaaccg ctcttaactg
      721 tgattacaag catcggacac gatcatatga acattttggg aaacaccatt gaagaaattg
      781 caggagaaaa ggccggcatt attaaagaag gtattccaat cgttacagca gttacccagc
      841 cggaagcttt acaggtcatc cgccatgaag ccgaacggca tgctgcgccg ttccagtcat
      901 tgcatgatgc atgtgttatt ttcaatgaag aggctctgcc tgcaggagaa cagttttcat
      961 tcaaaacgga agagaaatgc tatgaagata ttcggacatc tctgattggc acccatcaaa
     1021 gacaaaatgc tgctttgtcc attttggctg cggagtggct caataaggag aatatcgccc
     1081 atatcagtga cgaagcgtta aggagcgggc ttgtgaaggc tgcttggccg ggacggttag
     1141 agcttgttca ggaacatcct ccggtatatt tagacggtgc gcataacgaa gaaggtgttg
     1201 aaaagctggc ggaaacgatg aagcagcgtt tcgccaattc acgcatttct gtggtgttta
     1261 gcgcgttaaa ggacaaaccg tatcagaaca tgattaaaag actggaaacc attgctcatg
     1321 cgattcattt tgcttctttt gatttcccgc gtgcttccct tgcaaaagat ctctatgatg
     1381 caagtgaaat cagcaataaa agctggagtg aagatccaga cgatgtaatc aaattcatag
     1441 aaagtaaaaa gggctcaaat gaaattgtgc tgattaccgg atctctttac tttatttctg
     1501 acattcgaaa aaggttgaaa taaaagcatc cggctccggc agaatcaaaa aaagattctg
     1561 ccgttttttt catgtgtaaa attgaatatt cccaattgga gctcaggatg tccattttgc
     1621 tacaatccat accagaactc aatgagtaaa ggtgtgttgt ctatgctatc cattcttttt
     1681 atcttcgggc ttatccttgg ttctttttac tatacggccg ggtgccgtat ccccttacat
     1741 ctatctatta ttgcgccccg ttcatcatgc ccgttttgcc ggcgaacatt aactcctgca
//