From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Plasmid ColVBtrp (from E. coli) replication protein A (repA) gene,
Date: 10 Sep 92 10:31:42 GMT
Distribution: bionet
Checksum: 25752     4

LOCUS       CLVREPAA     1525 bp ds-DNA             BCT       10-SEP-1992
DEFINITION  Plasmid ColVBtrp (from E. coli) replication protein A (repA) gene,
            complete cds.
ACCESSION   L01256
KEYWORDS    repA gene; replication protein A.
SOURCE      Plasmid ColVBtrp DNA.
  ORGANISM  Plasmid ColVBtrp
            Prokaryotae.
REFERENCE   1  (bases 1 to 1525)
  AUTHORS   Gibbs,M.D., Spiers,A.J. and Bergquist,P.L.
  TITLE     RepFIB: A basic replicon of large plasmids
  JOURNAL   Unpublished (1992)
  STANDARD  full staff_entry
FEATURES             Location/Qualifiers
     CDS             276..1253
                     /note="central protein required for replication"
                     /product="replication protein A"
                     /gene="repA"
                     /codon_start=1
                     /translation="MDKSSGELVTLTPNNNNTVQPVALMRLGVFVPTLKSLKNSKKNT
                     LSRTDATEELTRLSLARAEGFDKVEITGPRLDMDNDFKTWVGIIHSFARHNVIGDKVE
                     LPFVEFAKLCGIPSSQSSRRLRERISPSLKRIAGTVISFSRTDEKHTREYITHLVQSA
                     YYDTERDIVQLQADPRLFELYQFDRKVLLQLKAINALKRRESAQALYTFIESLPRDPA
                     PISLARLRARLNLKSPVFSQNQTVRRAMEQLREIGYLDYTEIQRGRTKFFCIHYRRPR
                     LKAPNDESKENPLPPSPAEKVSPEMAEKLALLEKLGITLDDLEKLFKSR"
BASE COUNT      385 a    382 c    366 g    392 t
ORIGIN
        1 gggccgggac cacgttcaca taagctgtgg catgttatgg ataaactgta acaggttcat
       61 gataagctgt attcagtaat gcatatactg aagtaagtta atgacataaa ctatggtcag
      121 tatgccagac tcagttgtta aatacagact gcaggttttt cttcagtcag ttgacggggt
      181 tctgacacac gattttctgt ttattctttt actgtccaca ggctggaggc tttctggaaa
      241 acgaaaattc agacatcaaa aaactgttcg gcgaggtgga taagtcgtcc ggtgaactgg
      301 tgacactgac accaaacaat aacaacaccg tacaacctgt ggcgctgatg cgtctgggcg
      361 tctttgtacc tacccttaag tcactgaaga acagtaaaaa aaatacactg tcacgcaccg
      421 atgccacgga agaactgacg cgtctttctc tggcccgtgc agaaggattc gataaggttg
      481 agatcaccgg cccccgcctg gatatggata acgatttcaa gacctgggtg gggatcattc
      541 attcctttgc ccgccataac gtgattggtg acaaagttga actgcctttt gttgagtttg
      601 caaaactgtg tggtatacct tcaagccagt catcccgcag gctgcgtgag cgcatcagcc
      661 cttccctgaa gcgcattgcc ggtaccgtga tctcgttttc ccgtaccgat gagaagcaca
      721 cccgggaata catcacccat ctggtacagt cagcctacta cgatactgag cgggatattg
      781 ttcagttaca ggctgatccc cgtctgtttg aactgtacca gtttgacaga aaagtccttc
      841 tccagcttaa ggcgattaat gccctgaagc gacgggagtc cgcccaggca ctctacacct
      901 ttatagagag cctgccccgg gatccggcac cgatatcgct ggcgcggctg cgtgcacgcc
      961 tcaatctgaa gtctcctgta ttttcccaga accagacggt cagacgggca atggagcagt
     1021 tgcgcgagat tggatatctt gattacacgg agatccagcg ggggcggaca aaattcttct
     1081 gtattcacta ccggcgtccc cggttaaaag cgccgaatga tgagagtaag gaaaatccgt
     1141 tgccaccttc acctgcggaa aaagtcagtc cggagatggc ggagaaactt gccctgcttg
     1201 aaaaactggg catcacactg gatgacctgg aaaaactctt caaatcccgc tgaacataaa
     1261 ctgtagtcag tgaagagtgc tcctttactg actacagctt atattatcag gtgcagtgag
     1321 tggtctgctc actgctgttt atattcagtt tcctgcagtg ctgcctgtgg ctgagctgcc
     1381 atctgcctgt cccttacgtg agtcaccccg taacctgatg ctgaggcatt gctcccttca
     1441 taaaacatgg tttactcact acagcttatc tacctgctcc agcttatgtt atgtctgttc
     1501 tgctgaccac agcttatata aggaa
//