From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Mengovirus hemagglutin structural protein (VP1-VP4) RNA.
Date: 12 Sep 92 10:02:54 GMT
Distribution: bionet
Checksum: 29242     9

LOCUS       MNGVP        2792 bp ss-RNA             VRL       12-SEP-1992
DEFINITION  Mengovirus hemagglutin structural protein (VP1-VP4) RNA.
ACCESSION   M88547
KEYWORDS    hemagglutinin; structural protein.
SOURCE      Mengovirus (strain 37A) RNA.
  ORGANISM  Mengovirus
            Viridae; ss-RNA nonenveloped viruses; Isometric ss-RNA viruses;
            Picornaviridae.
REFERENCE   1  (bases 1 to 2792)
  AUTHORS   Mann,L.M., Anderson,K., Luo,M. and Bond,C.W.
  TITLE     Molecular and structural basis of hemagglutination in mengovirus
  JOURNAL   Virology 190, 337-345 (1992)
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     5'UTR           1..89
                     /citation=[1]
     mutation        replace(11
                     /type="mutant 205, revertants 205A7, 205D2"
                     /note="'g' in mutation"
                     /citation=[1]
     misc_RNA        90..290
                     /product="leader peptide"
                     /note="putative"
                     /citation=[1]
     CDS             291..500
                     /note="ORF"
                     /product="structural protein"
                     /gene="VP4"
                     /note="putative"
                     /codon_start=1
                     /translation="GNSTSSDKNNSSSEGNEGVIINNFYSNQYQNSIDLSANATGSDP
                     PKTYGQFSNLLSGAVNAFSNMLPLLA"
                     /citation=[1]
                     /partial
     mat_peptide     291..500
                     /product="structural protein"
                     /gene="VP4"
                     /note="putative"
                     /codon_start=1
                     /citation=[1]
     CDS             501..1278
                     /note="ORF"
                     /product="structural protein"
                     /gene="VP2"
                     /note="putative"
                     /codon_start=1
                     /translation="DQNTEEMENLSDRVSQDTAGNTVTNTQSTVGRLVGYGTVHDGEH
                     PASCADTASEKILAVERYYTFKVNDWTSTQKPFEYIRIPLPHVLSGEDGGVFGAALRR
                     HYLVKTGWRVQVQCNASQFHAGSLLVFMAPEYPTLDVFAMDNKWSKDNLPNGTRTQAN
                     RKGPFAMDHQNFWQWTLYPHQFLNLRTNTTVDLEVPYVNIAPTSSWTQHASWTLVIAV
                     VAPLTYSTGASTSLDITASIQPVRPVFNGLRHEVLSRQSPI"
                     /citation=[1]
                     /partial
     mat_peptide     501..1268
                     /product="structural protein"
                     /gene="VP2"
                     /note="putative"
                     /codon_start=1
                     /citation=[1]
     mat_peptide     1269..1961
                     /product="structural protein"
                     /gene="VP3"
                     /note="putative"
                     /codon_start=1
                     /citation=[1]
     CDS             1279..1961
                     /note="ORF"
                     /product="structural protein"
                     /gene="VP3"
                     /note="putative"
                     /codon_start=3
                     /translation="VTIREHAGTWYSTLPDSTVPIYGKTPVAPANYMVGEYKDFLEIA
                     QIPTFIGNKVPNAVPYIEASNTAVKTQPLAVYQVTLSCSCLANTFLAALSRNFAQYRG
                     SLVYTFVFTGTAMMKGKFLIAYTPPGAGKPTSRDQAMQATYAIWDLGLNSSYSFTVPF
                     ISPTHFRMVGTDLVNITNADGWVTVWQLTPLTYPPGCPTSAKILTMVSAGKDFSLKMP
                     ISPAPWSPQ"
                     /citation=[1]
                     /partial
     CDS             1962..2792
                     /note="In VP1 of mutants 205 and 280 residue K231 and P232
                     are replaced by R and S respectively."
                     /product="structural protein"
                     /gene="VP1"
                     /note="putative"
                     /codon_start=1
                     /translation="GVENAEKGVTENTDATADFVAQPVYLPENQTKVAFFYDRSSPIG
                     AFTVKSGSLESGFAPFSNQACPNSVILTPGPQFDPAYDQLRPQRLTEIWGNGNEETSE
                     VFPLKTKQDYSFCLFSPFVYYKCDLEVTLSPHTSGNHGLLVRWCPTGTPNKPTTQVLH
                     EVSSLSEGRTPQVYSAGPGTSNQISFVVPYNSPLSVLPAVWYNGHKRFDNTGYLGIAP
                     NSDFGTLFFAGTKPDIKFTVYLRYKNMRVFCPRPTVFFPWPTSGDKIDMTPRAGVLML
                     E"
                     /citation=[1]
                     /partial
     mat_peptide     1962..2792
                     /product="structural protein"
                     /gene="VP1"
                     /note="putative"
                     /codon_start=1
                     /citation=[1]
     mutation        replace(2653..2655
                     /phenotype="hemagglutination minus"
                     /type="mutants 205 and 280"
                     /note="'gau' in mutation"
                     /product="VP1"
                     /citation=[1]
BASE COUNT      715 a    705 c    598 g    774 t
ORIGIN
        1 gctttacacg cgttgagtcg aggttaaaaa acgtctaggc cccccgaacc acggggacgt
       61 ggttttcctt tgaaaaccac gacaataata tggctacaac catggaacaa gagatttgtg
      121 ctcattccat gacctttgaa gaatgcccaa aatgctccgc cttacaatac agaaatggtt
      181 tctacctact gaagtatgat gaagagtggt accctgagga gttgttgact gatggtgaag
      241 atgatgtgtt cgatcctgat ttggacatag aagttgtgtt cgagacacaa ggcaattcaa
      301 cctcatccga taaaaacaat tcttcttccg agggtaatga aggagtgatt ataaataatt
      361 tctattccaa tcaataccaa aattcaattg atttatctgc caatgctact ggctctgacc
      421 ccccaaaaac ctatggccaa ttttcgaatc tgttgtcagg agcagtcaat gctttttcta
      481 acatgctccc cctccttgca gatcagaata cagaggagat ggagaatcta tcagaccgag
      541 tgtctcaaga cactgccggc aacacggtca caaacaccca atcaaccgtt ggtcgtcttg
      601 tcggatacgg aacagttcat gatggggaac atccagcttc ttgtgctgat actgcctcag
      661 agaaaattct ggcagttgaa agatattaca ctttcaaagt gaatgattgg acttccaccc
      721 agaaaccttt tgaatatatt cgtataccac taccacatgt tctatcaggt gaggatggtg
      781 gtgtctttgg agctgctctc cgaagacact accttgtaaa aactggctgg agagttcagg
      841 tccagtgtaa tgcatcccag tttcatgcgg ggagcctcct ggtctttatg gccccggagt
      901 atcctacact ggacgtgttt gcaatggaca acaaatggtc caaggacaat ctgcctaatg
      961 gaacaagaac acaagccaac aggaagggtc ctttcgctat ggaccaccaa aatttttggc
     1021 aatggactct ctatccgcac cagtttttaa atcttagaac taatacgacg gtagatttgg
     1081 aggtacctta tgtaaacatt gcccctacct cctcctggac tcaacatgct tcctggactt
     1141 tggtgattgc cgtggtagcc cccttgacct attcgactgg tgcttcaaca agccttgaca
     1201 tcaccgcctc aattcaacct gttcgtcccg tgtttaacgg actacggcat gaggtactgt
     1261 ctagacagtc tcctatccct gttaccatcc gggagcatgc tgggacctgg tattccactc
     1321 ttcctgatag cactgttccc atctatggaa aaactcctgt ggcccccgcg aattatatgg
     1381 taggagaata caaagacttc ttggagattg cccaaattcc gaccttcatt gggaacaaag
     1441 tgcccaatgc tgtcccatac attgaggctt ccaacacagc tgtcaagaca caaccacttg
     1501 ccgtttatca ggtaactctt tcatgttctt gcttggctaa cacattccta gccgctctct
     1561 cccggaattt tgcccagtac cgcggatcac tagtgtacac ttttgtgttc actgggactg
     1621 cgatgatgaa gggtaagttt cttattgcat acacccctcc cggcgccggg aaaccgacaa
     1681 gcagagatca ggccatgcaa gccacttacg ctatttggga tctaggtctg aattcttcgt
     1741 actcgtttac tgtgcctttc atttctccaa cccacttccg catggtggga actgatctag
     1801 ttaacattac taatgcggat ggttgggtca ctgtatggca gttgacccct cttacttacc
     1861 ctccaggttg ccctacctcc gcaaaaatcc tcacaatggt tagcgctggg aaggacttct
     1921 ctctaaaaat gcccatttct cctgcccctt ggagccctca aggcgtagaa aatgcagaga
     1981 agggtgtaac tgaaaataca gatgccacag ctgattttgt ggcacagcct gtttatttgc
     2041 ctgagaacca gactaaggta gcctttttct atgataggtc tagtcccatt ggagctttca
     2101 ctgtgaaatc tgggagctta gagtcgggat ttgccccttt ctccaaccag gcgtgtccca
     2161 attccgtcat tttaacacca gggccgcagt ttgatccggc ttatgatcaa ctgcgtccac
     2221 agcgtttgac cgaaatttgg ggcaatggga atgaggaaac ctccgaagtc ttccctctaa
     2281 aaactaagca ggattattcg ttttgtctct tctccccctt tgtgtattat aaatgtgacc
     2341 tggaagtgac cctaagtcca cacacctccg gtaatcacgg cctgttggtc cgctggtgcc
     2401 ctaccggaac tcccaacaag cccaccaccc aggtgctgca tgaggtaagt tctctctcag
     2461 aagggcgaac cccacaggtg tacagtgccg gacctggtac ttccaatcag atttcatttg
     2521 tagttcctta taattcgcct ttatctgttc tgcctgctgt ttggtataat gggcataaga
     2581 gatttgacaa cacaggctac ttgggaatag ctcccaattc tgatttcggc accctcttct
     2641 ttgctggaac gaaacctgat attaaattca ctgtatattt gagatacaaa aacatgaggg
     2701 tcttctgccc acgtccaact gttttctttc cttggcctac ttctggtgac aaaatagata
     2761 tgactcctag agcaggagtt cttatgcttg aa
//