From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Drosophilia melanogaster fructose 1,6 bisphosphate aldolase gene,
Date: 29 Jul 92 10:01:36 GMT
Distribution: bionet
Checksum: 13012    14

LOCUS       DROF16BP02   5159 bp ds-DNA             INV       29-JUL-1992
DEFINITION  Drosophilia melanogaster fructose 1,6 bisphosphate aldolase gene,
            complete cds.
ACCESSION   M98351 M76409
KEYWORDS    fructose 1,6 bisphosphate aldolase.
SEGMENT     2 of 2
SOURCE      Drosophila melanogaster (strain Oregon R) DNA.
  ORGANISM  Drosophila melanogaster
            Eukaryota; Animalia; Metazoa; Arthropoda; Uniramia; Insecta;
            Pterygota; Neoptera; Holometabola; Diptera; Brachycera;
            Cyclorrhapha; Schizophora; Drosophiloidea; Drosophilidae.
REFERENCE   1  (sites)
  AUTHORS   Tolan,D.R., Shaw-Lee,R. and Sullivan,D.T.
  TITLE     Generation of three isozymes of fructose 1,6 bisphosphate aldolase
            in Drosophila melanogaster by differential splicing
  JOURNAL   FASEB J. 5, 1532-1532 (1991)
  STANDARD  full staff_review
REFERENCE   2  (bases 1 to 5159)
  AUTHORS   Malek,A.A., Suter,F.X., Frank,G. and Brenner-Holzach,O.
  TITLE     Amino acid sequence of an invertebrate FBP aldolase (from
            Drosophila melangaster)
  JOURNAL   Biochem. Biophys. Res. Commun. 126, 199-205 (1991)
  STANDARD  full automatic
REFERENCE   3  (bases 1 to 5159)
  AUTHORS   Shaw-Lee,R., Lissemore,J.L., Sullivan,D.T. and Tolan,D.R.
  TITLE     Alternative splicing of fructose 1,6-bisphosphate aldolase
            transcripts in Drososphila melanogaster predicts three isozymes
  JOURNAL   J. Biol. Chem. 267, 3959-3967 (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     CDS             join(1127..1922,2015..2214,2524..2619)
                     /EC_number="4.1.2.13"
                     /product="fructose 1,6 bisphosphate aldolase"
                     /gene="aldolase 4A"
                     /codon_start=1
                     /translation="MTTYFNYPSKELQDELREIAQKIVAPGKGILAADESGPTMGKRL
                     QDIGVENTEDNRRAYRQLLFSTDPKLAENISGVILFHETLYQKADDGTPFAEILKKKG
                     IILGIKVDKGVVPLFGSEDEVTTQGLDDLAARCAQYKKDGCDFAKWRCVLKIGKNTPS
                     YQSILENANVLARYASICQSQRIVPIVEPEVLPDGDHDLDRAQKVTETVLAAVYKALS
                     DHHVYLEGTLLKPNMVTAGQSAKKNTPEEIALATVQALRRTVPAAVTGVTFLSGGQSE
                     EEATVNLSAINNVPLIRPWALTFSYGRALQASVLRAWAGKKENIAAGQNELLKRAKAN
                     GEAACGNYTAGSVKGFAGKDTLHVDDHRY"
     CDS             join(1127..1922,2015..2214,3716..3805)
                     /EC_number="4.1.2.13"
                     /product="fructose 1,6 bisphosphate aldolase"
                     /gene="aldolase 4B"
                     /codon_start=1
                     /translation="MTTYFNYPSKELQDELREIAQKIVAPGKGILAADESGPTMGKRL
                     QDIGVENTEDNRRAYRQLLFSTDPKLAENISGVILFHETLYQKADDGTPFAEILKKKG
                     IILGIKVDKGVVPLFGSEDEVTTQGLDDLAARCAQYKKDGCDFAKWRCVLKIGKNTPS
                     YQSILENANVLARYASICQSQRIVPIVEPEVLPDGDHDLDRAQKVTETVLAAVYKALS
                     DHHVYLEGTLLKPNMVTAGQSAKKNTPEEIALATVQALRRTVPAAVTGVTFLSGGQSE
                     EEATVNLSAINNVPLIRPWALTFSYGRALQASVLRAWAGKKENIAAGQNELLKRAKAN
                     GDAAQGKYVAGSAGAGSGSLFVANHAY"
                     /citation=[2]
     intron          order(M98352:1346..1503,1..1105)
                     /number=1
                     /note="2.5 kb gap"
                     /gene="aldolase"
     CDS             join(1127..1922,2015..2214,2840..2935)
                     /EC_number="4.1.2.13"
                     /product="fructose 1,6 bisphosphate aldolase"
                     /gene="aldolase 4C"
                     /codon_start=1
                     /translation="MTTYFNYPSKELQDELREIAQKIVAPGKGILAADESGPTMGKRL
                     QDIGVENTEDNRRAYRQLLFSTDPKLAENISGVILFHETLYQKADDGTPFAEILKKKG
                     IILGIKVDKGVVPLFGSEDEVTTQGLDDLAARCAQYKKDGCDFAKWRCVLKIGKNTPS
                     YQSILENANVLARYASICQSQRIVPIVEPEVLPDGDHDLDRAQKVTETVLAAVYKALS
                     DHHVYLEGTLLKPNMVTAGQSAKKNTPEEIALATVQALRRTVPAAVTGVTFLSGGQSE
                     EEATVNLSAINNVPLIRPWALTFSYGRALQASVLRAWAGKKENIAAGQNELLKRAKAN
                     SQACQGIYVPGSIPSFAGNANLFVAQHKY"
                     /citation=[2]
     mRNA            join(M98352:1272..1345,1106..1922,2015..2214,2524..3144)
                     /product="fructose 1,6 bisphosphate aldolase"
                     /gene="aldolase 4A"
     mRNA            join(M98352:1272..1345,1106..1922,2015..2214,2840..3175)
                     /gene="aldolase 4B"
     mRNA            join(M98352:1272..1345,1106..1922,2015..2214,3716..4472)
                     /gene="aldolase 4C"
     mRNA            join(M98352:1272..1345,1106..1922,2015..2214,3716..5066)
                     /gene="aldolase 4C"
     exon            1106..1922
                     /number=2
                     /gene="aldolase"
     intron          1923..2014
                     /number=2
                     /gene="aldolase"
     exon            2015..2214
                     /number=3
                     /gene="aldolase 4A"
     intron          2215..2523
                     /number=3A
                     /note="'alternatively spliced'"
                     /gene="aldolase"
     intron          2215..2839
                     /number=3B
                     /note="'alternatively spliced'"
                     /gene="aldolase"
     intron          2215..3715
                     /number=3C
                     /note="'alternatively spliced'"
                     /gene="aldolase"
     exon            2524..3144
                     /note="'encodes carboxyl-terminal amino acids 332-362 of
                     the isozymealdolase4A. Expressed only in adult tissues at
                     low levels.'"
                     /partial
     exon            2840..3175
                     /gene="aldolase 4B"
     polyA_signal    3117..3122
                     /evidence=EXPERIMENTAL
     polyA_site      3145
                     /note="'end of transcript of 1.6 kb encoding aldolase 4B;
                     alternativepolyadenylation.'"
                     /evidence=EXPERIMENTAL
     polyA_signal    3161..3166
                     /evidence=EXPERIMENTAL
     polyA_site      3175
                     /note="'end of 1.6 kb transcript encoding aldolase 4B;
                     alternativepolyadenylation.'"
                     /evidence=EXPERIMENTAL
     exon            3716..4472
                     /number=4C
                     /gene="aldolase 4C"
     exon            3716..5066
                     /number=4C
                     /gene="aldolase 4C"
     polyA_signal    4463..4468
                     /evidence=EXPERIMENTAL
     polyA_site      4473
                     /note="'end of 1.9 kb transcript encoding major aldolase
                     isozyme;alternativepolyadenylation.'"
                     /evidence=EXPERIMENTAL
     polyA_signal    4933..4938
     polyA_signal    4989..4994
     polyA_signal    5003..5008
     polyA_signal    5037..5042
                     /evidence=EXPERIMENTAL
     polyA_site      5068
                     /note="'end of 2.4 kb transcript encoding major aldoalse
                     isozyme;alternativepolyadenylation which is
                     developmentally controlled.'"
                     /evidence=EXPERIMENTAL
BASE COUNT     1614 a   1127 c    959 g   1459 t
ORIGIN
        1 aaataaaagt gaaagttacc acgtcagcca tatgcgggtg taagaagtca gcctccattc
       61 ggttagagta gacacaggat tccatgagct ttgattacat cagagggaat tattgccagg
      121 tttccgtgac tttgctcagt tacaaaaaca taatagtcga ggttaaaaaa aatttttctt
      181 tgtgatctaa gcttaaaaca attgttcggt ttttgcaaat agtttttagc aaattacaga
      241 cactataaaa taagacacta tataatattt cattccaaaa agtgcagtga tacttttcca
      301 actttgtata gtacaatcca agaggatttt tctaaaatgt gaattttcat tgatgtttgt
      361 gagaacctct aaaaaagctc tattaaaagg caacatttta tatgtgaatg ttgagagtgt
      421 aggacaaacc tagaaaaaaa aaaatgtgag agagagagag cgaagagctg cagatataga
      481 gaaaagcacg ttttccgtgc atgcgccttt aatctcattc aatcacccgc tctttgccat
      541 cgaatcagct gtgaaataca ctaccatgca aagcatttat tatcttcaat ggaaaaatat
      601 ttttaaattg gaaaaaacac cagtgacatt gacctgacac tgaaaacaaa attatataat
      661 accgcatcat taaacaacag catatgactc aatggctcta atcggttaac tcagagttcc
      721 actttaaata acttgacctt tacaaatatt ctttttattt tatggaaata ataattaggt
      781 cagttcagta aaataatcca acacttgatt gatagctatc tctgtagccg ttgttatctt
      841 tttcagtagg aacatatgta actttgagtt acttgatttt ggttgtcaga ctgtgcggat
      901 cgtatacgaa attttagtcc aaatttttag ttttattttt tacctcgaaa tatttcaaaa
      961 ttctgtgctt acgcaatggt ttccaatcat ccgcgccgct gcgaattcga tagctagctt
     1021 attaaaacac aatgtccgct actctttttc atccaacaaa ataacaccac accaaatcaa
     1081 acgtaaatgg atcttcgtgc tgcagaacta cactcgaatc tcaaaaatga cgacctactt
     1141 caactacccc agcaaggagc tgcaggatga gctgcgcgaa atcgcccaga aaatcgttgc
     1201 ccccggcaag ggaatcctcg ccgccgatga gtccggccca accatgggca agcgtctgca
     1261 ggacatcggc gtggagaaca ccgaggacaa ccgccgtgcc taccgtcagc tgttgttcag
     1321 cactgacccc aagctggccg agaacatctc tggagtgatc ctgttccacg agaccctcta
     1381 ccagaaggcc gatgatggca cccccttcgc cgagatcctg aagaagaagg gaatcattct
     1441 gggcatcaag gtcgacaagg gtgttgtccc actgttcggc tctgaggatg aggtcaccac
     1501 ccagggtctg gatgacctgg ccgcccgttg cgcccagtac aagaaggacg gttgcgactt
     1561 cgccaagtgg cgttgcgtcc tgaagatcgg caagaacacc ccatcctacc agtcgatcct
     1621 ggagaacgcc aatgtcctgg cccgctacgc ctccatctgc cagtcgcagc gcatcgtccc
     1681 aattgtggag cccgaggttc tgcccgatgg cgatcacgat ctggaccgcg cccagaaggt
     1741 caccgagacc gtcctggccg ccgtctacaa ggccctgagc gaccaccacg tctacctgga
     1801 gggtactctg ctgaagccca acatggtcac cgccggtcag tcggccaaga agaacacccc
     1861 cgaggagatc gccctggcca ccgtgcaggc tctgcgccgc accgttcccg ccgccgttac
     1921 tggtgagtca tcaaatccaa ctgtaatctc tggattggaa tgcattcacc ttgtttttgc
     1981 aatgaatcct atctaatcga ttgtttgtct acaggcgtga ccttcctgtc tggaggtcag
     2041 tccgaggagg aggccaccgt caacctgagt gccatcaaca acgttccctt gatccgccca
     2101 tgggccctca ccttctcgta cggtcgtgcc ctgcaggcct ccgtcctgcg tgcctgggct
     2161 ggcaagaagg agaacatcgc tgccggccag aacgagctgc ttaagcgcgc caaggtgagt
     2221 ccttggatag ttgtatctat gcacgattga ttgcagttag caatttaagt ggtgaatgta
     2281 ttaagagtgt caggtatatg cttcggaagt attccttggg ctaagaaata ttatacatgt
     2341 ataatataac atacagcata ttcccagcca ttgattccat ttccaatccc aaatgaccac
     2401 aatgttcttc taatcctcta atcttatccc ttttatttcc ctaccattat ttgtaacctt
     2461 tttttgcttt tgtacgtgag acattaattt catcatcaat aaccacacat taatcatgcc
     2521 taggctaacg gagaggcggc ttgtggtaac tatacagctg gatcggttaa ggggttcgct
     2581 ggaaaagata ctttgcatgt ggatgaccac aggtattgat caaaggattg aagaattgct
     2641 gccttaagat ggcgatgaac aagctttcat ggggtaactt aagaatcata gccgcctttc
     2701 accaaatccg acttatcgca aacgccttta acatactgtc ctcgtgccac tcatgcttcg
     2761 cttgctctaa acctaatcca caaaagccca aaatctgatt aatcccaacc aaaatcccgc
     2821 tctcctcccg cttccacagg ccaattccca ggcctgtcag ggtatttacg tgcccggctc
     2881 aattccgtcg tttgccggca atgccaacct ctttgtcgcc cagcacaaat actaagtcct
     2941 aaatgccacg cactaatccc gagtcacaat cgagcgaagc tatatcccaa gccgaatcca
     3001 gataatccgc aagagttact gatcgaggcc cagctggagt gagacgggca acatttatat
     3061 tcctccaaca atggaactgc aaatatattc cttttataca tttttacatt ttaccgaata
     3121 aactgactag accttaaaga aaatattttt ggcgaacaga aataaattat atatttctaa
     3181 taataccttg cttatgattt tgtttacttt agttagcttt aaacaaaaat tcgattttat
     3241 tttacctttt aatttaattt aaatacattt taattaaaaa tgtcttgtct aactttattc
     3301 atgaaagccc tttgcaaact tcctactgtt aattagaata ataattcgga atatatgtac
     3361 ctgacagtta agttgaaaaa caacgtgaaa ttcgttcgga gagctgttac atatatttaa
     3421 tcgaatcata attttcctcc aaagaacttg caagattaaa ttttccaaaa ctttccgcag
     3481 tacgcagcat gtattcgtgc gctttgatgc gtggaaagtt tttagaaaat tgccgcaggt
     3541 ggaaaaagtg gcggaagttc tttgagcgca gcaaaaatca gtgttaaaac tttttcatac
     3601 tggccgaaaa cttttttgct gcggttcgcc aagttacaac taagtaataa ttaggagtag
     3661 gcatttctca caacgaaatt aatcaatttt ccccgctttc tttaccctcc tccaggccaa
     3721 cggtgatgct gctcagggca agtacgttgc cggcagcgct ggtgccggat ctggatccct
     3781 gttcgtggcc aaccacgcct actaaggcgt ttggttggac cttggctaca cgttactaca
     3841 aaaaaaaaaa aatacgaagc agattgctgg aaaagtttgg cagatttgtt tatttttacc
     3901 ttagtttcga gagctaagca aagcaagcat tacaaaaaat aaccaaaaaa aaaagatgaa
     3961 cgattacact ttcaaaacat gttatatata tgtagtacgt gacatttttg caagtttatt
     4021 atattataac attattttag agaacaacta aatgatgagt gcagaagaaa ggattgaata
     4081 tgttcgatgg agaaacaatt caacaattca aacaatttta tctttagttt taagcacatg
     4141 aacctattat tgcttgaagt tattacattt ctattctatt attatgatta tgcaagattt
     4201 caatgaaaac aaaaggatat gtcgtaaatc gctaggctaa agcacatttt atggtgaaaa
     4261 caacagcaat cactatcagc aacacctaaa tgtatgaggc ttgtaacatt gaagtcgcac
     4321 aacgtttttt gaattttttt aatgttttat aaaaagatcc actatgaaaa tcgatgtttt
     4381 accattatta ttattattaa actttatatt tatgattgat aagtataagt gaaacaaatg
     4441 acgagataaa caaacacatt aaaataaacc cgaaaagccc cccaaacaac tttattaacc
     4501 ttatatacat atatttacat tgttaaaaga gaacttgttg ttgcaaaata ctaaaagaaa
     4561 tatatgaatt tatatattca acatttggac ttcagtgtcc aatggcttct aaactgtgct
     4621 ggggcacaat gcttgttact agaatttttt tgaaaccaac tctgattctc tatccaaaac
     4681 gcgatgggta gtaacaacgg cagcaacgac attatcacag tacatattta aatacaaaaa
     4741 tattgtacca atgactaacc cacatattta tcaaatcatg ttttaattta cacgttattc
     4801 aagttgcaaa ttctgcatat cgttgttatt cacacaattg tttgaagtgc aatgtgaaaa
     4861 tataacagac acgagtgaag gatatacaat aatacctacc ggaagggtta agcttaaaat
     4921 agtttattgt gaaataaaaa cccaccacaa acggcaacaa caaccagctg tcactccaat
     4981 atacaagaaa taaaaaacaa caaataaaca tcatccgcat acaagtaaca tctttcaata
     5041 aaacacctta agagcaaaga aacccgaatt ggtttaattt taatgggttg ttttaaaacg
     5101 cttgcatacc ctcccaggcc tgagttacag tttaaaaggt actgtgggct taaaccaag
//