From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: T.versutus genes cyc and coxI for cytochrome c(550) and
Date: 21 Jul 92 10:08:43 GMT
Distribution: bionet
Checksum: 25473     6

LOCUS       TFECYCCOXI   1313 bp ds-DNA             BCT       21-JUL-1992
DEFINITION  T.versutus genes cyc and coxI for cytochrome c(550) and
            cytochrome-c oxidase subunit I
ACCESSION   X62808
KEYWORDS    amicyanin; coxI gene; cyc gene; cytochrome C oxidase subunit I;
            cytochrome c-550; electron transfer protein; haem-containing;
            redox protein.
SOURCE      Thiobacillus versutus DNA.
  ORGANISM  Thiobacillus versutus
            Prokaryotae; Gracilicutes; Scotobacteria;
            Colorless sulfur bacteria.
REFERENCE   1  (bases 1 to 1313)
  AUTHORS   Canters,G.W.
  JOURNAL   Unpublished (1991)
  STANDARD  full automatic
REFERENCE   2  (sites)
  AUTHORS   Ubbink,M., van Beeumen,J. and Canters,G.W.
  TITLE     Cytochrome c-550 from Thiobacillus versutus: cloning, expression in
            Escherichia coli, and purification of the heterologous holoprotein
  JOURNAL   J. Bacteriol. 174, 3707-3714 (1992)
  STANDARD  full staff_review
COMMENT     *source: strain=ATCC 25364;
            From EMBL    entry TVCYCCOXI;  dated 10-JUN-1992.
FEATURES             Location/Qualifiers
     CDS             <1..468
                     /gene="unidentified ORF"
                     /codon_start=1
                     /translation="HAVGRGGQYTYHGPGQRLVYVMLDLNRRGRDVRAFVKALESWVI
                     DALAEFNLKGEIRDGRVGVWIARPDKASLPDGSMREDKIAAIGVKLRRWVSFHGISIN
                     VEPDLGHYAGIVPCGIQGHGVTSLVDMGLPVGMGDLDLALRRSFARNFPPLGG"
     misc_feature    475..480
                     /note="conserved stretch"
     misc_feature    505..514
                     /note="conserved stretch"
     misc_feature    510..519
                     /note="dyad symmetry A>"
                     /evidence=EXPERIMENTAL
     misc_feature    571..582
                     /note="conserved stretch"
     misc_feature    581..590
                     /note="dyad symmetry A<"
                     /evidence=EXPERIMENTAL
     RBS             602..605
     sig_peptide     613..672
     CDS             613..1077
                     /product="cytochrome c(550)"
                     /gene="cyc"
                     /evidence=EXPERIMENTAL
                     /codon_start=1
                     /translation="MKISIYATLAALSLALPAVAQEGDAAKGEKEFNKCKACHMVQAP
                     DGTDIVKGGKTGPNLYGVVGRKIASVEGFKYGDGILEVAEKNPDMVWSEADLIEYVTD
                     PKPWLVEKTGDSAAKTKMTFKLGKNQADVVAFLAQHSPDAGAEAAPAEGAAN"
     mat_peptide     673..1074
                     /product="cytochrome c(550)"
                     /gene="cyc"
                     /evidence=EXPERIMENTAL
     misc_feature    1097..1106
                     /note="dyad symmetry B>"
                     /evidence=EXPERIMENTAL
     misc_feature    1101..1106
                     /note="conserved stretch"
     misc_feature    1123..1133
                     /note="dyad symmetry B<"
                     /evidence=EXPERIMENTAL
     misc_feature    1131..1139
                     /note="conserved stretch"
     misc_feature    1192..1203
                     /note="conserved stretch"
     5'UTR           1200..1239
     RBS             1229..1232
     CDS             1240..>1313
                     /EC_number="1.9.3.1"
                     /note="subunit I"
                     /product="cytochrome-c oxidase"
                     /gene="coxI"
                     /codon_start=1
                     /translation="MADAAVHGHGDHHDTRGFFTRWFMS"
BASE COUNT      237 a    456 c    413 g    207 t
ORIGIN
        1 catgccgtcg gccgcggcgg gcaatacacc tatcacgggc cgggccagcg cctggtctat
       61 gtcatgctgg acctgaaccg ccgcggccgc gacgtgcgcg ccttcgtcaa ggccctggaa
      121 tcctgggtca tcgacgcgct ggccgagttc aacctcaagg gcgagatccg cgacggccgc
      181 gtcggcgtct ggatcgcgcg ccccgacaag gcgtccctgc ccgacggctc gatgcgcgag
      241 gacaagatcg ccgccatcgg cgtcaagctg cgccgctggg tcagcttcca cggcatttcg
      301 atcaacgtcg agccggacct gggccattac gccggcatcg tgccctgcgg catccagggc
      361 catggcgtga ccagcctggt ggacatgggc ctgcccgtcg gcatgggcga tctggacctg
      421 gcgctgcgcc gcagcttcgc gcgcaatttc cccccgctcg gcggctgatt ttcgccctgc
      481 gacatattca cccttcaccg tcctgcgacc tttttccggt gttcggacgg cttgacgcat
      541 gatagccctg tcaaggcggg aggcggagag gtccgctccc ccggaaaaag cgattaccca
      601 agaggaaacg cgatgaagat cagcatctat gccactctcg ccgccctgtc cctcgccctg
      661 cccgccgtgg cccaggaagg cgacgccgcc aaaggcgaga aggaattcaa caagtgcaag
      721 gcctgccaca tggtccaggc accggacggc accgacatcg tcaagggcgg caagaccggg
      781 ccgaacctct acggcgtcgt cggccgcaag atcgcctcgg tcgaaggctt caaatacggc
      841 gacggcatcc ttgaagtggc ggaaaagaac cccgacatgg tctggtccga ggccgacctg
      901 atcgaatacg tcaccgatcc caagccctgg ctggtcgaga agaccggcga ttcggccgcc
      961 aagaccaaga tgaccttcaa gctcggcaag aaccaggccg acgtggtcgc cttcctggcc
     1021 cagcactcgc ccgatgcggg tgccgaggcg gcccccgccg aaggcgccgc gaactgaccg
     1081 gcgacccccg gaacggcgcg cccctgcctg cggcaaaacc gcgcaggggc gcgactttta
     1141 gtcccttctt cgcattgcga ggggtaggaa tgtgggctag aaacttagcg agtccgctgg
     1201 cccttgggcc ggccagccgc atgtctaggg agtccacgca tggcagacgc agccgttcac
     1261 ggccacggtg accatcatga cacccgcggg ttcttcaccc gctggttcat gtc
//