From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: nif gene cluster: nifH=nitrogeanse Fe protein...nifY=24.273 kda
Date: 6 Jan 93 07:06:22 GMT
Distribution: bionet
Checksum: 16314 13

LOCUS       S111769      6071 bp    DNA             BCT       16-DEC-1992
DEFINITION  nif gene cluster: nifH=nitrogeanse Fe protein...nifY=24.273 kda
            protein [Azospirillum brasilense=nitrogen-fixing bacteria, Genomic,
            5 genes, 6071 nt]
ACCESSION   S44003
KEYWORDS    .
SOURCE      nitrogen-fixing bacteria
  ORGANISM  Azospirillum brasilense
            Unclassified.
REFERENCE   1  (bases 1 to 6071)
  AUTHORS   Passaglia,L.M., Nunes,C.P., Zaha,A. and Schrank,I.S.
  TITLE     The nifHDK operon in the free-living nitrogen-fixing bacteria
            Azospirillum brasilense sequentially comprises genes H, D, K, an
            353 bp orf and gene Y.
  JOURNAL   Braz. J. Med. Biol. Res. 24, 649-675 (1991)
  STANDARD  full automatic
COMMENT     This entry [NCBI gibbsq 111769] was created by the journal scanning
            component of NCBI/GenBank at the National Library of Medicine.
            This sequence comes from Fig. 2.
            Region: nif gene cluster.  
FEATURES             Location/Qualifiers
     CDS             361..1242
                     /note="NifH;  For the protein sequence (NCBI gibbsq
                     111770):  Method: conceptual translation supplied by
                     author.  This sequence comes from Fig. 2."
                     /gene="nifH"
                     /product="nitrogeanse Fe protein"
                     /codon_start=1
                     /translation="MSLRQIAFYGKGGIGKSTTSQNTLAALVELDQKILIVGCDPKAD
                     STRLILHAKAQDTVLHLAAEAGSVEDLELEDVLKIGYKGIKCVESGGPEPGVGCAGRG
                     VITSINFLEENGAYDDVDYVSYDVLGDVVCGGFAMPIRENKAQEIYIVMSGEMMALYA
                     ANNIAKGILKYAHSGGVRLGGLICNERQTDKEIDLASALAARLGTQLIHFVPRDNIVQ
                     HAELRRMTVIEYAPDSQQAQEYRQLANKVHANKGKGTIPTPITMEELEEMLMDFGIMK
                     SEEQQLAELQAKEAAKA"
     CDS             1383..2831
                     /note="NifD;  For the protein sequence (NCBI gibbsq
                     111771):  Method: conceptual translation supplied by
                     author.  This sequence comes from Fig. 2."
                     /gene="nifD"
                     /product="nitrogenase MoFe protein a subunit"
                     /codon_start=1
                     /translation="MSLSVNEGVDVKGLVDKVLEAYPEKSRRRRAKHLNVLEAEAKDC
                     GVKSNIKSIPGVMTIRGCAYAGSKGVVWGPIKDMIHISHGPVGCGYYSWSGRRNYYVG
                     DTGVDKLGTMHFTSDFQEKDIVFGGDKKLHKVIEEINELFPLVNGISIQSECPIGLNG
                     DDIEGVSKAKSEELGKPVVPVRCEGFRGVSQSLGHHIANDVIRDWILPKKTEPKEGFV
                     STPYDVTIIGDYNIGGDAWASRILLEEIGLRVIAQWSGDGTLAELENQPKAKVNLIHS
                     YRSMNYIARHMEEKFGIPWMEYNFFGPSQIAESLRKIAALFDDTIKENAEKVIAKYQP
                     MVDAVVGDAALRAHGTKGRVPLKPLGPRHRPIVDAYHDLGMEIVGTGYEFAHNDDYQR
                     TQHYVKEGTLIYDDVTAFELEKFVELMRPDLVASGIKEKYVFQKMGLPFRQMHSWDYS
                     GPYHGYDGFAIFARDMDLAINNPVWGIMKAPF"
     CDS             2856..4421
                     /note="NifK;  For the protein sequence (NCBI gibbsq
                     111772):  Method: conceptual translation supplied by
                     author.  This sequence comes from Fig. 2."
                     /gene="nifK"
                     /product="nitrogenase MoFe protein b subunit"
                     /codon_start=1
                     /translation="MSMSHPVSQSADKVIDHFTLFRQPEYKELFERKKTEFEYGPTPT
                     KEVARVSAWTKTEEYKEKNLPVEAVVINPTKACQPIGAMLAAQGFEGTLPFVHGSQGC
                     VSYYRTHLTRHFKEPNSAVSSSMTEDAAVFGGLNNMIDGLQRYALYKPKMIAVLTTCM
                     AEVIGDDLSGFINNAKNKESVPADFPVPFAHTPAFVGSHIVGYDNMIKGVLTHFWGTS
                     ENFDTPKNETINLIPGFDGFAVGNNRELKRIAGLFGIQMTILSDVSDNFDTPADGEYR
                     MYDGGTPLEATKEAVHAKATISMQEYCTPQSLQFIKREGPAGRQAYNYPMGVTGTDEL
                     LMKLAELSGKPSRGVKLERGRLVDAIADSHTHLHGKRFAVYGDPDFCLGMSKFLMELG
                     AEPVHILSTSGSKKWEKQVQKVLDACRSASSGKAYGAKDLWHLRSLVFTDKVDYIIGN
                     SYGKYLERDTKIPLIRLTYPIFDRHHHHRYPTWGYQGALNVLVRILDRIFEDMDANTN
                     IVGETDYSFDLVR"
     CDS             4630..4986
                     /note="Description: orf1 3' of nifK;  12.323 kda nif-like
                     protein;  For the protein sequence (NCBI gibbsq 111773):
                     Method: conceptual translation supplied by author.  This
                     sequence comes from Fig. 2."
                     /codon_start=1
                     /translation="MAHLTQSVEYASTACCWLAGGRHAAEQPGSGGIAGNLPAPSSPR
                     SFRSWRRRGSSRPAKGSRVTACQGAGRPSASSSSSTPSRARSRCCECQEIRGRCAVFD
                     DRPPDWATSASAPSTP"
     CDS             5231..5914
                     /note="24.273 kda protein;  For the protein sequence (NCBI
                     gibbsq 111774):  Method: conceptual translation supplied
                     by author.  This sequence comes from Fig. 2."
                     /gene="nifY"
                     /product="NifY"
                     /codon_start=1
                     /translation="MAARAPAARTLLCPAFRRTHSHDPADRQSPVPFRRLWPPCPRPR
                     RGLPAATTSRSPSSRPTPQLHIALPTRRAGRHAPDLAALFEAVGVRHVPGVVTTIHAD
                     AARWTSPHRRRPVHPALRPLRPGRRSRLFTPPIPGLAEHAFNVDAGGVTTSTGERIRP
                     PPSSGRGAAANPVGAADSPASMTVRARSADPFLAGDRHATASSSTGDVALAARRPRQR
                     CRHVPASTL"
BASE COUNT     1177 a   2073 c   1840 g    981 t
ORIGIN      
        1 atgacatggg ccaggacggc gacgagttgg aggggttggc gctgcgcctc gtcgtcgccc
       61 gcgacgcgct gaaccacgcc gccgcctgac gctggctctg acgctggcgg accgagattc
      121 gtccatccct tccttttcat tgcgagagac ccgacaaaat gtcgggcttt gtcgggtatg
      181 tcacaggccc gacaaagcgg accgcgctcg tccgcaatca attttcttgt tcaatttcaa
      241 tgatttaaaa atttttcgcg aactggcacg ggggatgcag agaaggggtc gaagcggccg
      301 ctggcaggcc gccccggaat tgaagacacc ctgtagaccc aagcaaagga gtaacctccc
      361 atgtctttgc gccagattgc gttctacggt aagggcggta tcggcaagtc caccacctcc
      421 cagaacaccc tggccgcgct ggtcgagctg gatcagaaga tcctgatcgt cggctgcgat
      481 ccgaaggccg actcgacccg cctgatcctg cacgccaagg cgcaggacac cgtgctgcac
      541 ctcgccgccg aagccggctc ggtcgaggat ctggagctcg aggacgttct caagatcggc
      601 tacaagggca tcaagtgcgt cgagtccggc ggtccggagc cgggggtcgg ctgcgccggc
      661 cgcggcgtga tcacctcgat caacttcctg gaagagaacg gcgcctacga cgacgtggac
      721 tacgtctcct acgacgtgct gggcgacgtg gtgtgcggcg gtttcgccat gcccatccgc
      781 gagaacaagg cccaggaaat ctacatcgtc atgtccggtg agatgatggc gctctacgcc
      841 gccaacaaca tcgccaaggg cattctgaag tacgcccaca gcggcggcgt gcgcctcggc
      901 ggcctgatct gcaacgagcg ccagaccgac aaggaaatcg acctcgcctc cgccctggcc
      961 gcccgcctcg gcacccagct catccacttc gtgccgcgcg acaacatcgt gcagcacgcc
     1021 gagctgcgcc gcatgacggt gatcgagtac gcgccggaca gccagcaggc ccaggaatac
     1081 cgccagctcg ccaacaaggt ccacgcgaac aagggcaagg gcaccatccc gaccccgatc
     1141 acgatggaag agctggaaga gatgctgatg gacttcggca tcatgaagtc ggaggagcag
     1201 cagctcgccg agctccaggc caaggaagcc gccaaggcct gataactggg tcccccacgg
     1261 ggcgcagttc tgaactggcc ccctccccag cccaccccgc ccctgtgcgc ggggtgggag
     1321 agagggggct cggtcccgcg ctgcagtagc gcggataatg agtgcagcag gagactggca
     1381 ccatgagcct gtccgtgaac gaaggcgtcg acgtcaaggg tctcgtcgac aaggttctcg
     1441 aagcgtatcc cgagaagtcg cgcaggcgcc gcgccaagca cctgaacgtg ctggaggccg
     1501 aggcgaagga ctgcggcgtc aagtcgaaca tcaagtcgat ccccggtgtg atgaccatcc
     1561 gtggttgcgc ctacgccggt tccaagggtg tggtgtgggg tccgatcaag gacatgatcc
     1621 acatctccca cggcccggtc ggctgcggct actactcctg gtccggccgc cgcaactact
     1681 atgtcggcga caccggcgtg gacaagctag ggacaatgca cttcacctcc gacttccagg
     1741 agaaggacat cgtcttcggc ggcgacaaga agctgcacaa ggtgatcgag gagatcaacg
     1801 agctgttccc gctcgtcaac ggcatctcca tccagtccga gtgcccgatc ggcctgaatg
     1861 gcgacgacat cgaaggcgtg tccaaggcca agtccgagga actgggcaag ccggtcgttc
     1921 cggtccgttg cgaaggcttc cgcggcgtct cccagtcgct gggccaccac atcgccaacg
     1981 acgtcatccg cgactggatc cttccgaaga agaccgagcc gaaggaaggc ttcgtctcca
     2041 ccccgtacga cgtcaccatc atcggtgact acaacatcgg tggcgacgcc tgggccagcc
     2101 gaatcctgct ggaagagatc ggcctgcgcg tgatcgccca gtggtcgggc gacggcacgc
     2161 tcgccgagtt ggagaaccag ccgaaggcga aggtgaacct catccactcg taccgctcga
     2221 tgaactacat cgcgcgccac atggaagaga agttcggtat tccgtggatg gagtacaact
     2281 tcttcggccc gtcgcagatc gccgagtccc tgcgcaagat cgccgctctc ttcgatgaca
     2341 ccatcaagga gaacgcggag aaggtcatcg ccaagtacca gccgatggtc gacgcggtag
     2401 taggcgacgc agccctgcga gcccatggga cgaagggcag ggtgcccctg aagcccctgg
     2461 ggccgcgaca tcggccgatc gtcgacgcct accatgacct gggcatggag atcgtcggca
     2521 cgggttacga gttcgcccac aacgacgact atcagcgcac ccagcactat gtgaaggaag
     2581 gcacgctgat ctacgacgac gtcaccgcct tcgaactgga gaagttcgtc gaactgatgc
     2641 gtccggacct cgtcgcttcg ggcatcaagg aaaagtacgt cttccagaag atgggcctgc
     2701 ccttccgcca gatgcactcc tgggattact cgggtccgta tcacggctac gacggcttcg
     2761 cgatcttcgc ccgcgacatg gatctggcca tcaacaaccc cgtctggggc atcatgaagg
     2821 ctccgttcta aagcggcctc tggaagatag gagtaatgag catgtcccac cccgtttccc
     2881 agagcgccga caaggtcatc gaccacttca cgctcttccg tcagcccgaa tacaaggagc
     2941 tgttcgagcg caagaagacg gagttcgagt acgggcccac tccgacgaaa gaggtcgccc
     3001 gggtttccgc gtggaccaag acggaagagt acaaggaaaa gaacttgccc gttgaggcgg
     3061 tcgtcatcaa cccgacgaag gcctgccagc cgatcggcgc gatgttggcc gcccagggct
     3121 tcgagggcac cctgcccttc gtccatggct cgcagggctg cgtctcctac taccgcacgc
     3181 acctcacccg ccacttcaag gagccgaact ccgcggtttc ctcgtcgatg acggaagacg
     3241 cggcggtgtt cggcggcctg aacaacatga tcgacggcct gcaacgctac gcgctgtaca
     3301 agccgaagat gatcgccgtg ctgaccacct gcatggccga ggtcatcggc gacgacctct
     3361 ccggcttcat caacaacgcg aagaacaagg aaagcgtccc ggcggatttc ccggttccct
     3421 tcgcccacac cccggccttc gtcggcagcc acatcgtcgg ctacgacaac atgatcaagg
     3481 gtgttctgac ccacttctgg ggcacgtcgg agaacttcga caccccgaag aacgagacga
     3541 tcaacctgat cccggggttc gacggtttcg cggtcggcaa caaccgcgag ctgaagcgca
     3601 tcgccggcct gttcggcatc cagatgacca tcctgtcgga cgtgtcggac aacttcgaca
     3661 ccccggccga cggcgagtac cgcatgtatg acggcggcac cccgctggag gccacgaagg
     3721 aggccgtcca cgccaaggcc accatctcca tgcaggaata ctgcaccccg cagtccctgc
     3781 agttcatcaa gcgagaaggg ccagcaggtc gccaagcgta caactacccg atgggcgtca
     3841 ccggcaccga cgagcttctg atgaagctgg ccgagctgtc cggcaagccg tcccgcggag
     3901 tgaagctgga gcgcggccgt ctcgtcgacg ccatcgccga cagccacacc cacctgcacg
     3961 gcaagcgctt cgccgtgtac ggcgacccgg acttctgcct gggcatgtcg aagttcctga
     4021 tggagctggg cgccgagccg gtgcacatcc tgtccacctc cggctcgaag aagtgggaga
     4081 agcaggttca gaaggtgctc gacgcctgcc gttcggcaag ttccggcaag gcctacggcg
     4141 caaaggacct ctggcacctg cgctcgctgg tcttcaccga caaggtcgac tacatcatcg
     4201 gcaacagcta cggcaagtat ctggagcgcg acaccaagat cccgctcatc cgcctgacct
     4261 acccgatctt cgaccgtcac caccatcacc gttatccgac ctggggctac cagggcgctc
     4321 tgaacgtgct ggtgcgcatc ctggatcgga tcttcgagga tatggacgcc aacacgaaca
     4381 tcgtcggcga gaccgactac tcgttcgatc tggtgcgctg atctcccccg gccggcgcgg
     4441 cctccatccc cccgcccggc cttccccgga gagccggagc ccaccgcaag tgggctcggc
     4501 ttttccatgt ccggcctgcc acctcgccca taaactcgga taaacactta tccgcgtttt
     4561 ccgtcttgcg cttgggaacg cggactgata cggtctgtgt tcgtcgagag ccgttggacg
     4621 ggccacgcca tggcacacct cacgcagagc gtcgaatacg catccactgc ctgctgctgg
     4681 ctggccggcg gccgacacgc cgctgagcag ccgggatctg gcggaattgc agggaatctc
     4741 cccgctcctt cctcgccaag atctttccga agctggagaa ggcggggatc gtcacggcca
     4801 gcgaaggggt cgcgggttac cgcctgccaa ggcgccggaa gaccatcagc ttcctcgtca
     4861 tcgtcgacgc catcgagggc gagaagccgc tgttgcgagt gccaggagat ccgcggccgt
     4921 tgcgcggtct tcgacgacag accgccggac tgggcgacct ccgcgtctgc gccatccacg
     4981 ccgtgatgct gaaggccgag aaggcaatgc gcgacacctg gccaaggaga ctctggccag
     5041 cgtcggcgaa acctgtcggc aagccccgcc ggaattccag ggcgacgtcc agacctggat
     5101 cgacgcgcgc ctcacgccgc gccgcgtcac ggacacggca tacaccgtag ctggccaccc
     5161 tagccggccc aaccatcacg cctcacccga tgccctgaaa cacctcccgg acctccgccg
     5221 gacggacggg atggctgccc gcgcacccgc ggccagaacg ctactctgtc cggctttcag
     5281 aaggacacac agtcatgacc cagcagatcg tcaatcgccg gttccgtttc gccgattgtg
     5341 gccgccttgt ccgcgcccgc gccgtgggct gccggccgcg acgacgtcgc gatcaccgtc
     5401 gtctcgcccg acccctcagc tccacatcgc cctgcctacg aggcgtgctg gaaggcatgc
     5461 gccggacctc gccgcgctgt tcgaggccgt gggtgtccgc cacgtccccg gcgtcgtgac
     5521 aacgatccac gccgacgccg cgcggtggac gtcaccgcac cgacggcgcc cggttcaccc
     5581 tgccctacga ccgcttcgtc ctggccggcg cagccgcctg ttcaccccgc ccattcccgg
     5641 tctcgccgag cacgccttca acgtcgacgc tgggggcgtc accacctcca ccggcgagcg
     5701 cattcggccg ccaccgtcgt ctggacgcgg agccgcggcc aatcccgttg gcgccgcaga
     5761 ctcaccggcg agcatgacgg ttcgggcgcg ttccgccgat ccgttccttg cgggcgatcg
     5821 gcacgcgacg gcatcttcgt ccaccggcga cgtggcgttg gccgcacgac gaccgcggca
     5881 acgctgccgc catgttcctg ccagcacgct ttgagcctgg gcggtccggg gcacaacgcc
     5941 gcggcagctt ggtcggcctg ccaggcccac ccctacagcc agcccaatac gtgactgcct
     6001 gacctcggac ctgggcgtct caagacggct gggatccgac ctggctgacc gcgagaggca
     6061 atggcggcta a
//