From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: P. fluorescens gene for triacylglycerol lipase, complete cds.
Date: 15 Sep 92 10:05:10 GMT
Distribution: bionet
Checksum: 18570     5

LOCUS       PSETAL       1595 bp ds-DNA             BCT       15-SEP-1992
DEFINITION  P. fluorescens gene for triacylglycerol lipase, complete cds.
ACCESSION   D11455
KEYWORDS    lipase; triacylglycerol acylhydrolase; triacylglycerol lipase.
SOURCE      Pseudomonas fluorescens (strain SIK W1) (library: pUC19) DNA, clone
            pJH92.
  ORGANISM  Pseudomonas fluorescens
            Prokaryotae; Gracilicutes; Scotobacteria; Pseudomonadaceae.
REFERENCE   1  (bases 1 to 1595)
  AUTHORS   Chung,G.H., Lee,Y.P., Jeohn,G.H., Yoo,O.J. and Rhee,J.S.
  TITLE     Cloning and nucleotide sequence of thermostable lipase gene from
            Pseudomonas fluorescens SIK W1
  JOURNAL   Agric. Biol. Chem. 55, 2359-2365 (1991)
  STANDARD  full staff_review
COMMENT     These data kindly submitted in computer readable form by:
            Rhee Joon Shick
            Dept. of Biotechnology
            Korea Advanced Institute of Science and Technology
            Yusung-Gu, Kusung-Dong 373-1
            Taejon 305-701
            Korea
            Phone:  042-869-2613
            Fax:    042-869-2610
FEATURES             Location/Qualifiers
     RBS             143..146
     CDS             154..1503
                     /EC_number="3.1.1.3"
                     /product="triacylglycerol lipase"
                     /evidence=EXPERIMENTAL
                     /codon_start=1
                     /translation="MGVFDYKNLGTEASKTLFADATAITLYTYHNLDNGFAVGYQQHG
                     LGLGCRHTGRGVARQHRLPGSDPPAFPGILTRKRPPWTRCTQPVGRQSSASALGYGGK
                     VDARGTFFGEKAGYTTAQAEVLGKYDDAGKLLEIGIGFRGTSGPRESLITTPCRSGQR
                     PARRAGPQGLCEKLCRRTFGGLLKTVADYAGAHGLSGKDVLVSGHSLGGLAVNSMADL
                     STSKWAGFYKDANYLAYASPTQSAGDKVLNIGYENDPVFRALDGSTFNLSSLGVHDKA
                     HESTTDNIVSFNDHYASTLWNVLPFSIANLSTWVSHLPSAYGDGMTRVLESGFYEQMT
                     RDSTIILCPTWSDPARANTWVQDLNRNAEPHTGNTFIIGSDGNDLIQGGKGADFIEGG
                     KGNDTIRDNSGHNTFLFSGHFGQDRIIGYQPTGWCSRAPTAAPTCATTRRPWGPIRC"
     sig_peptide     154..222
                     /codon_start=1
BASE COUNT      331 a    508 c    486 g    270 t
ORIGIN
        1 aagcttgcat gcctgcagac aagccccctg ttgcaaacaa accgcctgag gcaagccgac
       61 cccctgtggc gagcgggctt gcccgttggg ctgcgcagca gcaataaaac cacacaatct
      121 ccggccaatt ccaacaacaa gagagacaag acaatgggtg tatttgacta caagaacctc
      181 ggcaccgaag ccagcaaaac cttgttcgcc gatgccaccg caatcacgtt gtatacctat
      241 cacaacctgg ataacggctt cgcagtcggc taccagcaac atggcttggg gctcggctgc
      301 cggcacactg gtcggggcgt tgctcggcag cacagactcc cagggagtga tcccccggca
      361 ttccctggaa tcctgactcg gaaaaggccg ccctggacgc ggtgcacgca gccggttgga
      421 cgccaatcaa gcgccagcgc actgggctac ggcggcaagg tggatgcgcg gggcactttt
      481 tttggcgaga aggccggcta caccacggcc caggccgaag tgctgggcaa gtacgatgac
      541 gccggcaaac tgctcgagat cggcatcggt tttcgtggca cctcgggccc tcgggaaagc
      601 ctgattacga ctccatgccg atctggtcag cgacctgctc gccgcgctgg gccccaagga
      661 ctatgcgaaa aactatgccg gcgaacgttt ggcggcttgc tcaagacggt ggccgactat
      721 gccggcgccc atggcctgag tggcaaggat gtgctggtca gcggccacag cctgggcggc
      781 ctggcggtca acagcatggc cgacctgagc accagcaaat gggcgggttt ctacaaggac
      841 gccaactacc tggcctacgc ctcgcccacc cagagcgccg gcgataaggt cctgaatatc
      901 ggctacgaaa acgacccggt attccgtgcg ctggacggct ccaccttcaa cctgtcgtcc
      961 ctcggcgtgc atgacaaggc ccacgagtcg accaccgaca acatcgtcag cttcaacgac
     1021 cactacgcct cgacgttgtg gaatgtgctg ccgttttcca tcgccaacct gtcgacctgg
     1081 gtgtcgcatt tgcccagcgc ttacggcgac ggcatgacgc gtgtgctgga atcggggttc
     1141 tacgagcaaa tgacccgtga ctcgacgatt atcctgtgcc caacctggtc cgacccggcg
     1201 cgcgccaaca cctgggtcca ggacctcaac cgcaatgccg agccgcacac aggcaatacc
     1261 ttcatcatcg gcagcgacgg caatgacctg atccagggcg gcaagggcgc ggacttcatc
     1321 gaaggcggca agggcaatga cacgatccgc gacaacagcg ggcacaacac ctttttgttc
     1381 agcgggcatt ttggccagga tcggattatc ggctaccagc cgacaggctg gtgttccagg
     1441 gcgccgacgg cagcaccgac ctgcgcgacc acgcgaaggc cgtgggggcc gatacggtgc
     1501 tgagttttgg cgccgactcg gtgacctggt cggggtcggg ctgggcggcc tgtggagcga
     1561 gggtgtgctg atccccgggt accgagctcg aattc
//