From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Winter squash mRNA for 1-aminocyclopropane-1-carboxylate synthase
Date: 29 Dec 92 00:50:08 GMT
Distribution: bionet
Checksum: 07496 5

LOCUS       CUCACCW      1699 bp ss-mRNA            PLN       04-DEC-1992
DEFINITION  Winter squash mRNA for 1-aminocyclopropane-1-carboxylate synthase
            (EC 4.4.1.14).
ACCESSION   D01032
KEYWORDS    1-aminocyclopropane-1-carboxylate; accW.
SOURCE      Winter squash (cv. Ebisu) fruit, mesocarp, parenchyma, cDNA to
            mRNA, clone pCMW33.
  ORGANISM  Cucurbita maxima
            Eukaryota; Plantae; Embryobionta; Magnoliophyta; Magnoliopsida;
            Dilleniidae; Violales; Cucurbitaceae.
REFERENCE   1  (bases 1 to 1699)
  AUTHORS   Nakajima,N., Mori,H., Yamazaki,K. and Imaseki,H.
  TITLE     Molecular cloning and sequence of a complementary DNA encoding
            1-aminocyclopropane-1-carboxylate synthase induced by tissue
            wounding
  JOURNAL   Plant Cell Physiol. 31, 1021-1029 (1990)
  STANDARD  full automatic
COMMENT     Submitted (23-MAY-1991) to DDBJ by: Hidemasa Imaseki School of
            Agricultural Sciences Nagoya University Chikusa-ku, Nagoya Aichi
            464-01 Japan Phone:  052-781-5111 x6268 Fax:    052-781-4447 Telex:
             442-2120.
FEATURES             Location/Qualifiers
     CDS             14..1495
                     /note="1-aminocyclopropane-1-carboxylate synthase"
                     /codon_start=1
                     /translation="MEFHQIDERNQALLSKIAVDDGHGENSPYFDGWKAYDNDPFHPE
                     DNPLGVIQMGLAENQLSFDMIVDWIRKHPEASICTPKGLERFKSIANFQDYHGLPEFR
                     NGIASFMGKVRGGRVQFDPSRIVMGGGATGASETVIFCLADPGDAFLVPSPYYAAFDR
                     DLKWRTRAQIIRVHCNSSNNFQVTKAALEIAYKKAQEANIKVKGVIITNPSNPLGTTY
                     DRDTLKTLVTFVNQHDIHLICDEIYSATVFKAPTFISIAQIVEEMEHCKKELIHILYS
                     LSKDMGLPGFRVGIIYSYNDVVVRRARQMSSFGLVSSQTQHLLAAMLSDEDFVDKFLA
                     ENSKRLAERHARFTKELDKMGITCLNSNAGVFVWMDLRRLLKDQTFKAEMELWRVIIN
                     EVKLNVSPGSSFHVTEPGWFRVCFANMDDNTVDVALNRIHSFVENIDKKEDNTVAMPS
                     KTRRRENKLRLSFSFSGRRYDEGNVLNSPHTMSPHSPLVIAKN"
BASE COUNT      477 a    366 c    380 g    476 t
ORIGIN      
        1 caacaacttt caaatggagt ttcatcaaat cgacgaaagg aaccaagctc ttctctctaa
       61 gatcgctgtt gacgatggcc atggcgagaa ctccccatat ttcgacgggt ggaaagctta
      121 cgataacgat ccgtttcacc ctgaggataa tcctttgggt gttattcaaa tgggtttagc
      181 agaaaatcag ctttcctttg atatgattgt tgactggatt agaaaacacc ccgaagcttc
      241 gatttgtaca ccgaaaggac ttgagagatt caaaagcatt gccaactttc aagattacca
      301 tggcttacca gagtttcgaa atgggattgc gagttttatg gggaaggtaa ggggtggaag
      361 ggtacaattc gacccgagtc ggattgtgat gggtggcggt gcgaccggag cgagcgaaac
      421 cgtcatcttt tgtttggcgg atccggggga tgcttttttg gttccttctc catactatgc
      481 tgcatttgat cgagatctga aatggcgaac acgagcacaa ataattcggg tccattgcaa
      541 cagctcgaac aacttccaag tcacaaaggc agccttagaa atagcctaca aaaaggctca
      601 agaggccaac atcaaagtga agggtgttat aatcaccaat ccctcaaatc ccttaggcac
      661 aacatacgac cgtgacactc ttaaaaccct cgtcaccttt gtgaatcaac acgacattca
      721 cttaatctgc gacgaaattt actctgccac tgtcttcaaa gccccaacct tcatcagcat
      781 cgcacagatt gttgaagaaa tggagcattg caagaaggag ctcatccata ttctttatag
      841 cttgtccaaa gacatgggcc tccctggttt tcgagttgga attatttatt cttacaacga
      901 tgtcgtcgtc cgccgtgctc ggcagatgtc gagcttcggc ctagtctcgt cccagactca
      961 acatttgctc gccgccatgc tttccgacga ggactttgtc gacaaatttc ttgccgagaa
     1021 ctcgaagcgc ctcgccgaga ggcatgcaag gttcacaaaa gaattggata aaatggggat
     1081 cacttgcttg aacagcaatg ctggagtttt tgtgtggatg gatctacgga ggctattaaa
     1141 agaccaaacc ttcaaagctg aaatggagct ttggcgtgtg attatcaacg aagtcaagct
     1201 caatgtttct cctggctcat cctttcatgt caccgagcca ggttggtttc gagtttgttt
     1261 cgcaaacatg gacgacaaca ctgtcgacgt tgctctcaat aggatccata gctttgtcga
     1321 aaacattgac aagaaggaag acaataccgt tgcaatgcca tcgaaaacga ggcgtcgaga
     1381 gaataagtta cgattgagct tctccttctc agggagaaga tacgatgagg gcaacgttct
     1441 taactcaccg cacacgatgt cgcctcactc gccgttagta atagcaaaaa attaattaaa
     1501 aatatttttc acaatattca tactattcat atagtttttt ttttgggtca tgttgactaa
     1561 agttacgtac attttttcca cagtggatat ggtgtaagct tcatattttt tggtgggatg
     1621 gtgatagatg taatgtattt ggtttttccc tcagggaact catacttatt tattaatgaa
     1681 atgattgtga ttatgaatt
//