From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Clostridium perfringens cpa gene for phospholipase C (alpha-toxin)
Date: 25 Jul 92 10:03:03 GMT
Distribution: bionet
Checksum: 10314     5

LOCUS       CLOA         2034 bp ds-DNA             BCT       25-JUL-1992
DEFINITION  Clostridium perfringens cpa gene for phospholipase C (alpha-toxin)
            (EC 3.1.4.3)
ACCESSION   X13608
KEYWORDS    alpha-toxin; cpa gene; phospholipase c; toxin.
SOURCE      Clostridium perfringens DNA.
  ORGANISM  Clostridium perfringens
            Prokaryotae; Firmicutes; Endospore-forming rods and cocci;
            Bacillaceae.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Leslie,D.L., Fairweather,N.F., Pickard,D., Dougan,G. and Kehoe,M.
  TITLE     Phospholipase C and hemolytic activities of clostridium perfringens
            alpha-toxin, cloned in Escherichia coli: sequence and homology with
            a Bacillus cereus phospholipase C
  JOURNAL   Mol. Microbiol. 3, 383-392 (1989)
  STANDARD  full automatic
COMMENT     *source: strain=CN 1491; *source: clone=pDAZ3;
            From EMBL    entry CPA;  dated 17-JUN-1992.
FEATURES             Location/Qualifiers
     CDS             785..1981
                     /product="phospholipase C"
                     /gene="cpa"
                     /codon_start=1
                     /translation="MKRKICKALICAALATSLWAGASTKVYAWDGKIDGTGTHAMIVT
                     QGVSILENDLSKNEPESVRKNLEILKENMHELQLGSTYPDYDKNAYDLYQDHFWDPDT
                     DNNFSKDNSWYLAYSIPDTGESQIRKFSALARYEWQRGNYKQATFYLGEAMHYFGDID
                     TPYHPANVTAVDSAGHVKFETFAEERKEQYKINTAGCKTNEAFYTDILKNKDFNAWSK
                     EYARGFAKTGKSIYYSHASMSHSWDDWDYAAKVTLANSQKGTAGYIYRFLHDVSEGND
                     PSVGKNVKELVAYISTSGEKDAGTDDYMYFGIKTKDGKTQEWEMDNPGNDFMTGSKDT
                     YTFKLKDENLKIDDIQNMWIRKRKYTAFSDAYKPENIKIIANGKVVVDKDINEWISGN
                     STYNIK"
BASE COUNT      773 a    276 c    357 g    628 t
ORIGIN
        1 gaattccaag accatcgata ccttcactta ttatatattc tctaacgata ggtgcaaggg
       61 cagcctttcc atctccacct cttgaataga ttgttctacc agaccctttt aattaacatc
      121 atatctttta ctatctttag ttacatgttc tcctaataag agtgctctac catctcctag
      181 cattgtaaaa tgaccaaatt ggtcgcccag cataagcctg tgcaattggt acaatcctgg
      241 aagttcattc ctgcaaatat attaagtcca aaatcgctgt ttaaaacttc ttcattaagc
      301 ccaagttctt cagcaagaga agtattaaac ttaataagtt taggattttt tgaacccttt
      361 ggattttgtt cactaaagaa tatatttgga agagttaaat aagtgttttc taagttaaaa
      421 cctgtttttg attgaaaatt tttattatcc atattaaaat cctttgcctt ataatttatt
      481 tcaaatttta tccatccctt atattatgtg taaaaattct tattaaatta aaaaacaaga
      541 tttaacttat tatagcacta ataattgtaa attttcatat taaaaataag tttaacaatt
      601 tagagtgggt aaggttagat atgtttaatt gaaatttgaa ttgtattcaa aaatatttta
      661 aaaaatattc aaaaatttag tgagcttatg gtaattatat ggtataattt cagtggcaag
      721 tgttaatcgg ttatcaaaaa aggggagatt aatacttgaa aaaaattaac gggggatata
      781 aaaaatgaaa agaaagattt gtaaggcgct tatttgtgcc gcgctagcaa ctagcctatg
      841 ggctggggca tcaactaaag tctacgcttg ggatggaaag attgatggaa caggaactca
      901 tgctatgatt gtaactcaag gggtttcaat cttagaaaat gatctgtcca aaaatgaacc
      961 agaaagtgta agaaaaaact tagagatttt aaaagagaac atgcatgagc ttcaattagg
     1021 ttctacttat ccagattatg ataagaatgc atatgatcta tatcaagatc atttctggga
     1081 tcctgataca gataataatt tctcaaagga taatagttgg tatttagctt attctatacc
     1141 tgacacaggg gaatcacaaa taagaaaatt ttcagcatta gctagatatg aatggcaaag
     1201 aggaaactat aaacaagcta cattctatct tggagaggct atgcactatt ttggagatat
     1261 agatactcca tatcatcctg ctaatgttac tgccgttgat agcgcaggac atgttaagtt
     1321 tgagactttt gcagaggaaa gaaaagaaca gtataaaata aacacagcag gttgcaaaac
     1381 taatgaggct ttttatactg atatcttaaa aaacaaagat tttaatgcat ggtcaaaaga
     1441 atatgcaaga ggttttgcta aaacaggaaa atcaatatac tatagtcatg ctagcatgag
     1501 tcatagttgg gatgattggg attatgcagc aaaggtaact ttagctaact ctcaaaaagg
     1561 aacagcggga tatatttata gattcttaca cgatgtatca gagggtaatg atccatcagt
     1621 tggaaagaat gtaaaagaac tagtagctta catatcaact agtggtgaga aagatgctgg
     1681 aacagatgac tacatgtatt ttggaatcaa aacaaaggat ggaaaaactc aagaatggga
     1741 aatggacaac ccaggaaatg attttatgac tggaagtaaa gacacttata ctttcaaatt
     1801 aaaagatgaa aatctaaaaa ttgatgatat acaaaatatg tggattagaa aaagaaaata
     1861 tacagcattc tcagatgctt ataagccaga aaacataaag ataatagcaa atggaaaagt
     1921 tgtagtggac aaagatataa acgagtggat ttcaggaaat tcaacttata atataaaata
     1981 ataaaagtaa aaaaataatt attggttttg gtggtattta caaaataaaa gctt
//