From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Plasmid pTAR (from Agrobacterium tumefaciens strain 1D1422)
Date: 12 Jan 93 05:49:43 GMT
Distribution: bionet
Checksum: 42270 4

LOCUS       PT1PTAR2     1259 bp ds-DNA             BCT       11-JAN-1993
DEFINITION  Plasmid pTAR (from Agrobacterium tumefaciens strain 1D1422)
            partitioning protein (parA) gene, complete cds.
ACCESSION   X05121
KEYWORDS    parA gene; partition protein.
SEGMENT     2 of 2
SOURCE      Agrobacterium tumefaciens (strain 12D12) plasmid pTAR DNA, clone
            pUCD105.
  ORGANISM  Plasmid pTAR
            Prokaryotae.
REFERENCE   1  (bases 1 to 1259)
  AUTHORS   Gallie,D.R. and Kado,C.I.
  TITLE     Agrobacterium tumefaciens pTAR parA promoter region involved in
            autoregulation, incompatibility and plasmid partitioning
  JOURNAL   J. Mol. Biol. 193, 465-478 (1987)
  STANDARD  full automatic
COMMENT     [1]  enum. 1 to 1259.
               The 248 base-pair region upstream from the 23,500 Mr open
            reading frame contains an array of 12 seven-base-pair palindromic
            repeats involved in parA autoregulation
               Data kindly reviewed (02-NOV-1987) by KADO C.I.
FEATURES             Location/Qualifiers
     CDS             271..939
                     /note="parA protein product;  (AA 1-333)"
                     /codon_start=1
                     /translation="MPVVVVASSKGGAGKSTTAVVLGTELAHKGVPVTMLDCDPNRSL
                     TIWANAGEVPENITALSDVTESSIVKTIKQHDVDGAVVIVDLEGVASRMVSRAISQAD
                     LVLIPMRPKALDATIGAQSLQLIAEEEEAIDRKIAHAVVFTMVSPAIRSHEYTGIKAS
                     LIENGVEIIEPPLVERTAYSALFQFGGNLHSMKSKQGNMAAAIENAEAFAMAIFKKLT
                     EALR"
BASE COUNT      336 a    304 c    345 g    274 t
ORIGIN      About 0.8 kb after segment 1.
        1 gaattccccg cattgaaaat taactgcttc tttactttag ccagtcggca tatccgattt
       61 gatgcgatac ccggttcatt gaaataccgg gtaatttcgc ttatccggta attgtaatta
      121 tcggatttgt gtaaataccc ggttgcaatc aaaaccgggt gtgtgtttaa atccggtcaa
      181 ttcatttacc ggatttaatt attaatccga tatcaacttt taccgggtat ttgaataaac
      241 cagattatcg atttaaccgg agaatgacat atgcctgttg tcgtagtggc ttcatcaaag
      301 ggcggggcag ggaaatccac caccgccgtg gtgctgggaa ccgagcttgc gcataagggt
      361 gtccccgtca ccatgttgga ttgcgaccct aaccggtcat tgactatttg ggcaaacgcg
      421 ggggaagtcc cggagaatat cacagctcta agcgacgtaa ccgaatccag catcgtcaag
      481 acgatcaagc agcatgacgt tgatggagcc gtggtcattg ttgaccttga aggtgtagcg
      541 tcgcgcatgg tctctagagc aatatctcaa gcagacttgg tgctaattcc aatgcgcccc
      601 aaggcactgg acgcaaccat aggcgcgcag tcgctacagc tgattgcaga agaagaagaa
      661 gcaatagacc ggaaaatagc tcatgcggtc gtgttcacca tggttagccc cgcgatccgt
      721 tcacatgaat acaccggcat caaggcatca ctcatcgaaa acggagtaga aatcatcgaa
      781 ccgccacttg tagagcggac ggcatattcc gcgctttttc agttcggcgg caatctccat
      841 tcaatgaaat cgaagcaagg caatatggcg gcggcaatcg aaaacgccga ggccttcgcc
      901 atggcaatat ttaagaagct aacggaggca ctgcgatgaa tgatttcaaa ctagatctag
      961 ggtcgctgaa acggaccgcg aagccggcca gcgaagcggc catcgccaag ctgacgcagt
     1021 aggggaggcg agggggttcg ttgatcggtc gccgcgcgga aagcccggtc gcaagaagag
     1081 cgagcgcacc ggccaggtgc acgccaaatg ctgccgcacg tttccgaaga aatcgcagag
     1141 gaagctatgc ggcgtggcgt cgtgcaaggc gtcattatag aggaagcatg ggcactgtat
     1201 ttgaggccaa aggatgacgg agcaaatgcg ctacaggatt gaacattccg tcgagggcc
//