From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Lactobacillus casei D-alanine-activating enzyme gene, complete cds
Date: 31 Jul 92 10:01:42 GMT
Distribution: bionet
Checksum: 07146     6

LOCUS       LBADAAE      2383 bp ds-DNA             BCT       31-JUL-1992
DEFINITION  Lactobacillus casei D-alanine-activating enzyme gene, complete cds
            and a potential basic membrane protein (involved in D-ala-LTA
            biosynthesis) gene, 5' end.
ACCESSION   M83993
KEYWORDS    D-alanine-activating enzyme; basic membrane protein.
SOURCE      Lactobacillus casei (library: ATCC 7469) DNA.
  ORGANISM  Lactobacillus casei
            Prokaryotae; Firmicutes; Regular asporogenous rods;
            Lactobacillaceae.
REFERENCE   1  (bases 1 to 2383)
  AUTHORS   Heaton,M.P. and Neuhaus,F.C.
  TITLE     Biosynthesis of D-alanyl-lipoteichoic acid: Cloning, nucleotide
            sequence, and expression of the Lactobacillus casei gene for the
            D-alanine-activating enzyme
  JOURNAL   J. Bacteriol. 174, 4707-4717 (1992)
  STANDARD  full staff_entry
FEATURES             Location/Qualifiers
     CDS             292..1812
                     /product="D-alanine activating enzyme"
                     /gene="dae"
                     /codon_start=1
                     /translation="MIDNVITAIDRVAAEHPTRVAYDYEGTQYTYAQLKEGSDRLAGF
                     FAESLPAGEPIIVYGGQTFDMVEVFLGLSKSGHAYIPIDTHSPNERITQVQDVAHAPA
                     VIEVAPLPITVPDVKIIRAPALHQAEQSHAPIHSLQHAVAGDDNYYIIFTSGTTGKPK
                     GVQISHDNLLSYVNWNISDFGLEEGVVAMSQPPYSFDLSVMDLYPTLVLGGTLKALPK
                     EVTDNFKELFATLPKLGLNEWVSTPSFVEIALLDPNFKQENYPNLTHFLFCGEELVNK
                     TAQALITRFPKATVYNTYGPTEATVAVTGMAITQAIVDQYPRLPIGYAKPDTNVYVVD
                     EQGEQVSAGTEGELMIVGPSVSKGYLNNPDKTAAAFFKAGNQRGYRSGDLVTMTADGM
                     VFYRGRTDFQVKLHGYRIELEDVDHNLNQVSYIKQASTVPRYDKDHKVAQLIAFAVAK
                     PNDFDSEMKLTQAIKAELGKMVMEYMIPQRIIYRDQLPLTANGKVDRKALIAEVNH"
     CDS             1812..>2375
                     /function="possibly involved in D-ala-LTA biosynthesis"
                     /note="putative"
                     /product="basic membrane protein"
                     /gene="basic membrane protein"
                     /note="putative"
                     /codon_start=1
                     /translation="MLNLQPYENPQYFVYLIIALLPVIIGMFKGFRMHWYESIFSLVF
                     LVLIFDADKWPQGKALLGYVVFNLLLVYAYFKYRTREGSKNSTAVFYLSVALGIAHVA
                     VVKFTPLFQHHGSILGFLGISYLTFRVVGTIMEIRDGSIKDLNMWKFIQFLLFFPTIS
                     SGPIDRYRRFVKDYDRVPDPEHYAQLVT"
BASE COUNT      614 a    502 c    576 g    691 t
ORIGIN
        1 ggaattcctc taactggcct gtttgattca cattacgaaa ctgttaggtg attgttacgc
       61 ttttgcttgg cgaggctttt tctgtaaaac tataatggat gtgtcaataa gaacgtgggg
      121 tgacaagatg ttcgcaaaat taaaagcggt gtgggaaaat cccacatttc ggttcatctt
      181 ttggaccgtc acgtatttcg tgatactgct ggtcttggtt tatctatacg gttacagcgg
      241 tattaacaac agtaaattca tttataacga gttttaaagg gggaaaccgc gatgatcgat
      301 aatgttatta cagcgattga tcgggtcgcc gcagagcatc caacgcgcgt ggcttatgat
      361 tatgaaggaa cccaatatac ctacgcccag ttgaaagaag gcagtgaccg ccttgctgga
      421 ttctttgcgg aatcattgcc ggctggtgaa cccatcattg tctatggtgg tcaaaccttt
      481 gacatggttg aggttttcct tggactttct aaatcagggc atgcctacat tccgatcgac
      541 acgcatagtc caaatgaacg gattacccag gtgcaagatg ttgcccatgc gcctgctgtg
      601 attgaagtgg caccgcttcc gattacggtc ccagatgtca agattattcg ggcaccggcg
      661 ttacatcaag ctgagcagtc acacgcgcca attcattcac tgcagcatgc ggttgctggc
      721 gatgataatt actacatcat ctttacaagc ggcacaacgg ggaaaccaaa aggcgttcag
      781 attagtcacg ataacctttt gagctatgtg aactggaata tttccgactt tgggcttgaa
      841 gaaggcgttg tcgccatgtc tcagccgccg tattcctttg acctaagtgt gatggatctt
      901 tatccgacct tggttttagg cggcaccttg aaggctttac caaaagaagt cactgataac
      961 tttaaagaat tatttgcgac gttgccaaaa ttgggcttga acgaatgggt atcaacccct
     1021 tcttttgtcg aaattgcgtt acttgatccg aatttcaagc aggaaaatta tccgaacctg
     1081 acccattttc tgttttgcgg tgaggagcta gttaataaaa ccgcccaagc gctgatcaca
     1141 cgtttcccga aagcaaccgt ctataacacg tatggtccga ccgaagccac cgttgcggtt
     1201 acgggaatgg cgattaccca ggcgattgtt gatcaatatc cgcggttacc gattggctat
     1261 gcgaagcctg ataccaacgt ttatgtggtt gatgagcaag gtgaacaggt ttcagctggc
     1321 acagaaggcg aattgatgat cgtgggtcca agcgtgtcga aagggtatct caataatccg
     1381 gacaaaacag ccgcggcatt tttcaaggct ggcaatcaac gcggttatcg gtctggtgac
     1441 ttagtgacca tgaccgcaga tgggatggtc ttttatcgcg gtcggactga tttccaggtt
     1501 aagttgcatg gttatcgcat tgagctagaa gatgttgatc ataatttgaa tcaggtttct
     1561 tatattaagc aggcatcaac ggttccgcgg tatgacaagg atcataaagt tgcccagttg
     1621 attgcctttg cggttgccaa accaaatgac tttgatagcg aaatgaaact aacgcaggca
     1681 attaaagccg aactagggaa aatggtgatg gaatatatga ttccccaacg gattatttac
     1741 cgggatcagt taccgttaac ggctaatggc aaggttgatc gcaaagcact gattgcagag
     1801 gtaaatcatt aatgcttaat ttacaaccgt atgagaatcc acagtatttt gtctatttaa
     1861 tcattgcctt gcttcccgtc atcatcggca tgttcaaagg ctttcggatg cattggtatg
     1921 aaagcatctt ttcgttggtt ttcctggtac tgatttttga tgccgataaa tggcctcaag
     1981 gaaaagcgtt attggggtat gtggttttca acttattact ggtttacgcg tatttcaaat
     2041 atcgaacgcg cgaaggctca aaaaactcca cggcagtctt ttatcttagt gtcgcgttag
     2101 gaattgcgca cgtggcagtt gtcaagttta cgccgctgtt tcaacatcat gggtctattc
     2161 ttggcttctt gggcattagt tatttaactt tccgagtggt cggcacgatt atggaaattc
     2221 gtgatgggtc gattaaagac ctgaacatgt ggaagtttat ccaatttctg ctattcttcc
     2281 caaccatctc aagtggcccg attgatcggt atcggcgatt tgtcaaagat tacgatcgcg
     2341 ttcctgatcc cgagcattac gcccagttgg tgaccggaat tcc
//