From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: S. cerevisiae gene encoding mRNA guanylyltransferase.
Date: 1 Sep 92 10:03:27 GMT
Distribution: bionet
Checksum: 01765     8

LOCUS       YSCCEG       3327 bp ds-DNA             PLN       01-SEP-1992
DEFINITION  S. cerevisiae gene encoding mRNA guanylyltransferase.
ACCESSION   D10263
KEYWORDS    mRNA capping enzyme; mRNA guanylyltransferase.
SOURCE      S. cerevisiae (strain DBY939) DNA, clone pYGT6.
  ORGANISM  Saccharomyces cerevisiae
            Eukaryota; Fungi; Eumycota; Hemiascomycetes; Endomycetales;
            Saccharomycetaceae.
REFERENCE   1  (bases 1 to 3327)
  AUTHORS   Shibagaki,Y., Itoh,N., Yamada,H., Nagata,S. and Mizumoto,K.
  TITLE     mRNA capping enzyme: Isolation and characterization of the gene
            encoding mRNA guanylyltransferase subunit from Saccharomyces
            cerevisiae
  JOURNAL   J. Biol. Chem. 267, 9521-9528 (1992)
  STANDARD  full staff_review
COMMENT     Submitted (20-Jan-1992) to DDBJ by:
            Kiyohisa Mizumoto
            School of Pharmaceutical Sciences
            Kitasato University
            9-1, Shirokane 5 chome
            Minato-ku, Tokyo 108
            Japan
            Phone:  03-3444-6161 x3260
            Fax:    03-3444-6198
FEATURES             Location/Qualifiers
     mRNA            <237..>1657
                     /note="CEG1 (ORF1) mRNA"
     CDS             278..1657
                     /EC_number="2.7.7.50"
                     /note="ORF1"
                     /product="mRNA guanylyltransferase"
                     /gene="CEG1"
                     /codon_start=1
                     /translation="MVLAMESRVAPEIPGLIQPGNVTQDLKMILCKLLNSPKPTKTFP
                     GSQPVSFQHSDVEEKLLAHDYYVCEKTDGLRVLMFIVINPVTGEQGCFMIDRENNYYL
                     VNGFRFPRLPQKKKEELLETLQDGTLLDGELVIQTNPMTKLQELRYLMFDCLAINGRC
                     LTQSPTSSRLAHLGKEFFKPYFDLRAAYPNRCTTFPFKISMKHMDFSYQLVKVAKSLD
                     KLPHLSDGLIFTPVKAPYTAGGKDSLLLKWKPEQENTVDFKLILDIPMVEDPSLPKDD
                     RNRWYYNYDVKPVFSLYVWQGGADVNSRLKHFDQPFDRKEFEILERTYRKFAELSVSD
                     EEWQNLKNLEQPLNGRIVECAKNQETGAWEMLRFRDDKLNGNHTSVVQKVLESINDSV
                     SLEDLEEIVGDIKRCWDERRANMAGGSGRPLPSQSQNATLSTSKPVHSQPPSNDKEPK
                     YVDEDDWSD"
     CDS             complement(1856..3208)
                     /product="ORF2"
                     /codon_start=1
                     /translation="MLRMSTSRFIGQRLFTTARSLQAAKPAPKGKTQGFSKKSSSVSS
                     YSSAKRVTPGSLYKNWTNTTHTAQLQQTAVPLALPIFNFDDISKTLNKVVSYSNKQYK
                     SLHHLGSFKKSQFNELFQKPVCLVREDATNSFLKKLVSHPVKKFIITGEPGVGKTVLL
                     SQAHAYAVDSKQIIINISYPELFLNGRNDFSYDDDLKLFIQPMYLKKLIRKILKANDP
                     ALLKSIELSKDYKFSNANPKNASVKPFVTLNKTKNTVLDLLSVMTHPHNRGKLMKAII
                     DELSVQSKVPIMFTVDNFSKVLTTAYSAYRNTENKQIYSLDLQMGKLMMDIISGETKF
                     ANGESSTILAISGVDRTNKTLPVALGKIPVDPYVTRYHYEPKFVELLQKGNVTEFEVP
                     KLNKQEVNELIDYYKQSNVLLDKDITGKKWENLIDEKYFLSGNGNPRELLKSLVLSHR
                     "
BASE COUNT     1032 a    562 c    688 g   1045 t
ORIGIN      chromosome VII
        1 cagctgaaag gcagatgatc tcaatatgtc ccgataggcc aattttgcca ccagatagtt
       61 cgatggcatt ttatttttag tccttttgaa agcaatatca taaaatatat atagttctcc
      121 atgatgttcg ggtcagtcgc tccgaagcgt aacctagtat aataaatagt tcattgcaga
      181 aaataacgaa agaaatggtg gaatacgatc tgttatatct aaactaaagc taactaacgg
      241 aataagcaaa tacgaatcga ccgctaattt aacaaatatg gttttagcaa tggaaagtag
      301 agtggcaccg gaaattcctg ggctcattca acctgggaat gtcacgcaag acttgaagat
      361 gatcctctgt aaattattga attccccaaa acctacgaaa acattccctg gttcccagcc
      421 tgtgtccttt cagcattctg atgtggaaga gaagctgctt gcgcatgatt actacgtttg
      481 tgagaaaaca gatggtctgc gggtgttgat gtttatagtg ataaatcctg tgacgggtga
      541 gcaaggatgc tttatgattg atagggaaaa taactattat ctggttaatg gatttaggtt
      601 tcccagatta ccccaaaaga agaaagaaga gctgctagag actcttcaag atggcacctt
      661 attagatggt gaacttgtca tacaaactaa cccaatgaca aaattacaag agttgcgtta
      721 tttaatgttc gattgtcttg ctatcaatgg tagatgtctc acacaatcac caacaagttc
      781 tagactagcc caccttggaa aagaattttt taaaccatac ttcgatttaa gagcagcgta
      841 ccctaatcgt tgtactactt ttccgttcaa aatttccatg aaacatatgg atttcagtta
      901 ccaattagta aaagttgcta aaagtttaga taaactacca catctttctg atggtctgat
      961 atttactcct gtgaaggcac cttacactgc cggcggaaaa gattcattgt tattaaaatg
     1021 gaagccagaa caagaaaaca ccgtggactt caaattgatt ttagatatcc caatggtgga
     1081 ggatccttct ttgcctaaag atgatcggaa caggtggtat tacaattatg acgttaagcc
     1141 agttttcagc ttatatgtct ggcaaggcgg agctgatgtc aattcacgtt taaaacattt
     1201 cgaccagcct ttcgatagga aggaatttga aatattagaa agaacataca gaaaatttgc
     1261 agagttgagc gtttcagatg aggaatggca aaatttgaag aacctagaac agccattaaa
     1321 tggtagaata gtagagtgcg caaaaaacca agagactggg gcgtgggaaa tgttaagatt
     1381 cagggatgat aagttaaatg gtaatcatac atcggtggtc cagaaagttt tggagagtat
     1441 caacgattca gtttcattgg aggacctcga ggaaattgtt ggtgatatta aaaggtgctg
     1501 ggacgagaga agagcaaata tggctggtgg tagtgggaga ccactaccgt ctcaaagtca
     1561 aaatgcgaca ttatctacct ctaagccagt ccattcacag cccccaagta atgataagga
     1621 gccaaaatat gtagacgagg atgattggtc ggattaggct aagaagtgtt cgtatcgaca
     1681 tatatcggtg taagaatttt gtgtatgaat aagatagaaa agcaaaagca attgcttttg
     1741 ctaaattaat tcagttagtt atctatttat atgtacatga gaaatgggag aattttgaga
     1801 agcgagggtg aaagtgttat atacgcatat acatgtatat atatgcgcat gcttatcacc
     1861 tatgggaaag gactaaactt ttcaataatt ctcttgggtt accgttacca cttaggaaat
     1921 atttttcgtc aatcaagttc tcccactttt ttcctgtgat atccttgtct aataaaacat
     1981 ttgattgttt gtagtaatcg attagttcgt taacttcctg tttgttcagt ttaggaacct
     2041 cgaattcggt gacattacct ttttggagca attctacgaa cttcggttca tagtgatatc
     2101 tagtaacata ggggtctact gggatcttgc ccaatgccac gggtaaagtt ttgtttgtcc
     2161 tgtcaacgcc ggatatagct aatatagtag aactctcacc attagcaaat ttcgtctctc
     2221 ctgaaataat gtccatcatc aatttaccca tttgtaaatc cagggaatat atttgtttat
     2281 tctctgtatt tctgtacgca gaatatgcag tagttaacac ttttgaaaaa ttatccactg
     2341 taaacatgat tggcaccttg gattggacag ataactcatc aataatggct ttcattagtt
     2401 ttccacgatt atgcgggtgg gtcattacgg ataacaaatc taaaactgta tttttcgttt
     2461 tgtttaatgt gacaaatggt tttacagagg cattctttgg gtttgcattc gagaacttgt
     2521 agtctttgga tagctcaata gattttaaca atgcagggtc atttgccttc aatattttcc
     2581 ttataagctt tttcaaatac atcggttgga tgaaaagctt taaatcatca tcataagaaa
     2641 agtcattcct accgttcagg aataattcag gatatgatat gttaataatg atttgcttgg
     2701 aatcaactgc atatgcatgc gcttgagaga gtagtacagt tttcccgacc cctggctctc
     2761 cagtgattat aaatttttta acaggatgag atactagttt cttcaaaaag ctattcgttg
     2821 catcttcacg aactagacaa accggttttt gaaaaagttc attgaactgc gacttcttga
     2881 aggaacccaa atggtgaagg gatttgtact gtttgttgga atatgatact actttattca
     2941 aagtcttgga aatatcatca aagttgaaga taggtaatgc caaaggaacg gcggtctgtt
     3001 gtaattgagc ggtgtgcgtg gtgtttgtcc agtttttata caatgaacca ggagtaactc
     3061 tctttgcaga tgagtaagat gagactgaag atgatttttt ggaaaatcct tgagttttac
     3121 cttttggtgc cggtttagca gcctggagag atcgtgccgt agtgaacagc ctttgcccaa
     3181 tgaatcttga agttgacata cgtagcatta ttctgatacg tataccacgt ggcttgtact
     3241 tgttctcttg ttaatgtttt ccctcgaagt atagaagttg atgtatatat acaatataca
     3301 tatatatata ttcttgtaca tagtact
//