From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Eschericia coli hemB gene for porphobilinogen synthase (EC
Date: 22 Dec 92 22:51:09 GMT
Distribution: bionet
Checksum: 21449 4

LOCUS       ECOHEMB      1085 bp ds-DNA             BCT       30-NOV-1992
DEFINITION  Eschericia coli hemB gene for porphobilinogen synthase (EC
            4.2.1.24)
ACCESSION   X17417 M35121
KEYWORDS    hemB gene; heme biosynthetic enzyme; porphobilinogen synthase;
            synthase.
SOURCE      Escherichia coli DNA.
  ORGANISM  Escherichia coli
            Prokaryotae; Gram-negative facultatively anaerobic rods;
            Enterobacteriaceae.
REFERENCE   1  (bases 1 to 1085)
  AUTHORS   Echelard,Y., Dymetryszyn,J., Drolet,M. and Sasarman,A.
  TITLE     Nucleotide sequence of the hemB gene of Escherichia coli K12
  JOURNAL   Mol. Gen. Genet. 214, 503-508 (1988)
  STANDARD  full automatic
REFERENCE   2  (sites)
  AUTHORS   O'Neill,G.P., Thorbjarnardottir,S., Michelsen,U., Palsson,S.,
            Soell,D. and Eggertsson,G.
  TITLE     delta-Aminolevulinic acid dehydratase deficiency can cause
            delta-aminolevulinate auxotrophy in Escherichia coli
  JOURNAL   J. Bacteriol. 173, 94-100 (1991)
  STANDARD  full automatic
COMMENT     *source: strain=K12;
               From EMBL 27   entry ECHEMB;  dated 15-JAN-1991.
FEATURES             Location/Qualifiers
     -35_signal      15..20
                     /note="putative"
     -10_signal      41..46
                     /note="putative"
     misc_feature    53
                     /note="transcriptional start site"
     RBS             71..73
                     /note="pot. ribosome binding site; putative"
     CDS             80..1054
                     /gene="hemB"
                     /product="porphobilinogen synthase (AA 1-324)"
                     /codon_start=1
                     /translation="MTDLIQRPRRLRKSPALRVCLKRQHLALTTWCCRSLLKKKLTDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNPREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
BASE COUNT      254 a    277 c    301 g    253 t
ORIGIN      
        1 tcgcgacaac tttcgtaaaa catccctacc ctgcttcagg tatactatgc ccctcgattc
       61 cacaaacatc aggcagacca tgacagactt aatccaacgc cctcgtcgcc tgcgcaaatc
      121 tcctgcgctg cgcgtatgtt tgaagagaca acacttagcc ttaacgacct ggtgttgccg
      181 atctttgttg aagaagaaat tgacggacta caaagccgtt gaagccatgc caggcgtgat
      241 gcgcattcca gagaaacatc tggcacgcga aattgaacgc atcgccaacg ccggtattcg
      301 ttccgtgatg acttttggca tctctcacca taccgatgaa accggcagcg atgcctggcg
      361 ggaagatgga ctggtggcgc gtatgtcgcg catctgcaag cagaccgtgc cagaaatgat
      421 cgttatgtca gacacctgct tctgtgaata cacttctcac ggtcactgcg gtgtgctgtg
      481 cgagcatggc gtcgacaacg acgcgactct ggaaaattta ggcaagcaag ccgtggttgc
      541 agctgctgca ggtgcagact tcatcgcccc ttccgccgcg atggacggcc aggtacaggc
      601 gattcgtcag gcgctggacg ctgcgggatt taaagatacg gcgattatgt cgtattcgac
      661 caagttcgcc tcctcctttt atggcccgtt ccgtgaagct gccggaagcg cattaaaagg
      721 cgaccgcaaa agctatcaga tgaacccaat gaaccctcgt gaggcgattc gtgaatcact
      781 gctggatgaa gcccagggcg cagactgcct gatggttaaa cctgctggag cgtacctcga
      841 catcgtgcgt gagctgcgtg aacgtactga attgccgatt ggcgcgtatc aggtgagcgg
      901 tgagtatgcg atgattaagt tcgccgcgct ggcgggtgct atagatgaag agaaagtcgt
      961 gctcgaaagc ttaggttcga ttaagcgtgc gggtgcggat ctgattttca gctactttgc
     1021 gctggatttg gctgagaaga agattctgcg ttaattttgt ttcctctcag aaagggggag
     1081 ttaac
//