From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: S.pombe pho4 gene for thiamin-repressible acid phosphatase
Date: 24 Jul 92 10:11:05 GMT
Distribution: bionet
Checksum: 12224     6

LOCUS       YSPPHO4G     2789 bp ds-DNA             PLN       24-JUL-1992
DEFINITION  S.pombe pho4 gene for thiamin-repressible acid phosphatase
ACCESSION   X56939
KEYWORDS    acid phosphatase; phosphatase.
SOURCE      Schizosaccharomyces pombe DNA.
  ORGANISM  Schizosaccharomyces pombe
            Eukaryota; Fungi; Eumycota; Hemiascomycetes; Endomycetales;
            Saccharomycetaceae.
REFERENCE   1  (bases 1 to 2789)
  AUTHORS   Yang,J. and Schweingruber,M.E.
  TITLE     The structural gene coding for thiamin-repressible acid phosphatase
            in Schizosaccharomyces pombe
  JOURNAL   Curr. Genet. 18, 269-272 (1990)
  STANDARD  full automatic
REFERENCE   2  (bases 1 to 2789)
  AUTHORS   Schweingruber,M.E.
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
COMMENT     *source: clone=pSp4B; *source: sub_clone=pSp4B;
            From EMBL    entry SPPHO4G;  dated 15-JUN-1992.
FEATURES             Location/Qualifiers
     TATA_signal     662..669
     CDS             917..2308
                     /EC_number="3.1.3.2"
                     /note="thiamin-repressible acidphosphatase"
                     /product="acidphosphatase"
                     /gene="pho4"
                     /codon_start=1
                     /translation="MKLSGISLWLLAASIVHAGKSQFEAFENEFYFKDHLGTISVYHE
                     PYFNGPTTSFPESCAIKQVHLLQRHGSRNPTGDDTATDVSSAQYIDIFQNKLLNGSIP
                     VNFSYPENPLYFVKHWTPVIKAENADQLSSSGRIELFDLGRQVFERYYELFDTDVYDI
                     NTAAQERVVDSAEWFSYGMFGDDMQNKTNFIVLPEDDSAGANSLAMYYSCPVYEDNNI
                     DENTTEAAHTSWRNVFLKPIANRLNKYFDSGYNLTVSDVRSLYYICVYEIALRDNSDF
                     CSLFTPSEFLNFEYDSDLDYAYWGGPASEWASTLGGAYVNNLANNLRKGVNNASDRKV
                     FLAFTHDSQIIPVEAALGFFPDITPEHPLPTDKNIFTYSLKTSSFVPFAGNLITELFL
                     CSDNKYYVRHLVNQQVYPLTDCGYGPSGASDGLCELSAYLNSSVRVNSTSNGIANFNS
                     QCQAHSTNVTVYY"
     sig_peptide     917..970
BASE COUNT      809 a    532 c    475 g    973 t
ORIGIN
        1 cggcatgaaa caagtagctc taataaatta aattgttgga tcttactaaa gactaaatat
       61 taataataat tttctcccaa gctatttata tacatattag aagcaatttg caaagatagc
      121 agactaatac tcttcaatgc ccaacttctt attagtgata tacatgtaaa aatatcttta
      181 ttatgcaata ttatgtagca gctcgtacaa tgtttgcaca tctttacaca atagtattca
      241 ttttgtatta cattatcatt tataattcca tttcacagag agtaggcatt gctattatat
      301 aatataaatt tatataaaac aaaaagactg caaaatcatt tccaactgaa acttcgttct
      361 ttagttctat taaattatta agtattggaa tatcagattt ttgtaagttc agtaataata
      421 atatcattat attacaccgt tgtattatta atcgcataat attactgtaa cactttgtcc
      481 gtaatttgca tcgttatttc agttaacaat tgtgggtcca aaatcttaaa gtctaatagc
      541 gaactacacc aggtttgcaa cttcattcat tttttttaat tccaatgtag tcgtgccgaa
      601 atcagacttt ggtttggtgg tagccggggt gcttagtgtt agcagatatc cggattgatt
      661 gtatataaat atctgctgct tcttgcgctt agaatagtca tcttttgact gtttatttta
      721 gacttttcct acttcaaata atcaaagttc gttgattgaa tagactaccg ttcgtttcaa
      781 cccgctttta aatattcgat cagttatttt ttttgctatt gaatcgctgg tcgttggttg
      841 attcatacac agtaaatttc tttcttgtgg ttttattggg tctgcttggg ttacataaat
      901 tcattgtttt ttctcgatga agttaagtgg tatttctcta tggcttttgg ccgcatccat
      961 cgttcatgct ggtaaatctc aatttgaggc atttgaaaat gagttttatt tcaaggacca
     1021 tcttggaacg attagtgtat atcatgaacc ctactttaat gggcccacta cctccttccc
     1081 cgaatcttgt gcaattaaac aggttcattt gcttcagcgt catggatctc gaaacccgac
     1141 tggggatgac actgccactg atgtctctag tgcgcaatat atagatattt ttcaaaacaa
     1201 gcttctaaat ggctcaattc cggtaaattt tagctatcct gaaaatcctc tttacttcgt
     1261 taaacattgg actcctgtga taaaggctga aaatgccgac cagttgtcca gcagcggtcg
     1321 aattgaactt tttgacttgg gccgtcaagt ttttgagcgc tactatgagc ttttcgacac
     1381 tgatgtctac gatatcaata ctgctgctca agaacgtgtt gtcgactctg cagaatggtt
     1441 tagttatgga atgtttggcg atgatatgca gaacaaaacc aacttcatcg ttcttccgga
     1501 agacgattct gcgggtgcaa actccttagc tatgtattac tcttgtcccg tttatgaaga
     1561 caacaatatt gatgaaaaca ctactgaagc cgcccatact agttggcgta atgtttttct
     1621 caagcctatc gccaatcgct taaacaaata ctttgatagc ggttataatc ttacggtttc
     1681 ggatgttcgc agtctttact atatttgtgt atacgagatc gctctcagag ataacagcga
     1741 tttctgttca ctctttactc cctctgaatt tttgaatttt gaatatgaca gtgaccttga
     1801 ctacgcatat tggggtggtc ccgcttcaga atgggcttcc acattaggtg gggcttatgt
     1861 taataacctt gcaaataatc ttcgtaaggg tgtgaataat gcttccgatc gtaaggtatt
     1921 cttagcgttt acccacgact ctcaaattat tccagtcgag gctgctcttg ggtttttccc
     1981 cgacattact cctgagcacc ctctaccgac tgacaaaaac atttttacat actcattaaa
     2041 aacctcttcg ttcgttccct tcgcaggaaa cttaattact gaacttttct tatgttctga
     2101 taataaatac tatgtacgcc atctcgtcaa ccagcaagta taccccctta ctgattgcgg
     2161 ttatggtcct agtggtgctt cagatgggtt atgcgagttg agcgcttact tgaattcttc
     2221 ggttcgtgtt aattctacct ctaatggtat tgctaatttt aactcgcaat gtcaggcgca
     2281 ttccaccaac gttaccgtct actactagac ttagcatctt atattattgt taacacaatt
     2341 gggtccttag gaataatata gataattttt aactatagta atagcttatg tataagactt
     2401 aggtattaag gaggaaaagt agagcgaaca gaaagtgagc atttatatat tcaagaaaga
     2461 cttacgagac atagtataaa cgagttgtgt cagtgtttga acaaaaaagg aaatacttat
     2521 agttttaaat gcctagtacg aaatctataa ccttcactcg taccgccttt agtgaagcta
     2581 attcttcaat tcgacttact tcaaagcctt tcaagtgact ccgtagcttt tgtaattcct
     2641 ttccaaatca ttaatattaa taggtcggga taaatcttaa acatttctca agatataacg
     2701 cgtacattaa cgattccttt tggcgtcaac aactcacctt ttagccacac acgaacgaca
     2761 caaagtttaa tagaattcat ttaaaatat
//