From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Rat cystatin beta gene, complete cds.
Date: 31 Dec 92 01:39:39 GMT
Distribution: bionet
Checksum: 63067 6

LOCUS       RATCYB       2566 bp ds-DNA             ROD       17-DEC-1992
DEFINITION  Rat cystatin beta gene, complete cds.
ACCESSION   D10607 D90542
KEYWORDS    cystatin beta; cysteine proteinase inhibitor; family-1 cystatin;
            intracellular cystatin.
SOURCE      Rattus norvegicus (individual_isolate SHR, strain Wister) (library:
            lambda Charon 4A) DNA, clone GCy-2.
  ORGANISM  Rattus norvegicus
            Eukaryota; Animalia; Chorqata; Vertebrata; Mammalia; Theria;
            Eutheria; Rodentia; Myomorpha; Muridae; Murinae.
REFERENCE   1  (sites)
  AUTHORS   Sato,N., Ishidoh,K., Uchiyama,Y. and Kominami,E.
  TITLE     Molecular cloning and sequencing of cDNA for rat cystatin bata
  JOURNAL   Nucleic Acids Res. 18, 6698-6698 (1990)
  STANDARD  full automatic
REFERENCE   2  (bases 1 to 2566)
  AUTHORS   Sato,N., Ishidoh,K., Uchiyama,Y. and Kominami,E.
  TITLE     Structural organization of the gene encoding rat cystatin beta
  JOURNAL   Gene 114, 257-260 (1992)
  STANDARD  full automatic
COMMENT     Data kindly submitted in computer readable form by: Noboru Sato
            Department of Biochemistry Juntendo University School of Medicine
            Hongo 2-1-1 Bunkyo-ku Tokyo 113 Japan Phone:  03-3813-3111 x3515
            Fax:    03-3814-9300

               [1]  sites.
FEATURES             Location/Qualifiers
     repeat_unit     61..65
                     /rpt_type=tandem
     repeat_unit     66..70
                     /rpt_type=tandem
     GC_signal       81..86
                     /note="SP-1 binding site"
     repeat_unit     99..103
                     /rpt_type=tandem
     repeat_unit     104..108
                     /rpt_type=tandem
     repeat_unit     111..115
                     /rpt_type=tandem
     GC_signal       275..280
     repeat_unit     316..320
                     /rpt_type=tandem
     GC_signal       354..359
     exon            370..555
                     /number=1
     intron          556..1532
                     /number=1
     exon            1533..1634
                     /number=2
     intron          1635..2114
                     /number=2
     repeat_unit     1740..1924
                     /rpt_family="mouse B1 repetitive sequence"
     exon            2115..2430
                     /number=3
     polyA_signal    2408..2413
     CDS             join(490..555,1533..1634,2115..2243)
                     /gene="RCY-B"
                     /product="cystatin beta"
                     /codon_start=1
                     /translation="MMCGAPSATMPATTETQEIADKVKSQLEEKANQKFDVFKAISFR
                     RQVVAGTNFFIKVDVGEEKCVHLRVFEPLPHENKPLTLSSYQTDKEKHDELTYF"
BASE COUNT      652 a    635 c    674 g    605 t
ORIGIN      
        1 gaattccaga taaggtctta tttcatgggg ttatcaggac ccaaaaactc agtccaagtt
       61 ggggagggga ggacacgata gggcggatgt gcgtgttggg ggaggggaga ggggaaacag
      121 cgacctgaga cttctaggtc ctcaatcaac ggtttctgta cccaccctac aatcacttca
      181 cgaccattga caggcaatca gaactcccag cctcccaacc catcttagtg ccccttcagg
      241 gaaggggctt gaagtatcca ccctgcctca ccctccgccc acgcccagtc tgggtcttat
      301 gactctgcct ggaacgggga gagggtggtg acagtaaaga gagggtgcgg cctccgcccc
      361 ttcgctctgt cacgtgccac gggtccaggg gcggggccga gccaggtttt tctagggtcc
      421 agacacccag gtctcctagt tggctctctt cgtagcttct ctgtgatatt ctaaccagtg
      481 cttgccaaga tgatgtgtgg cgcgccatcc gccacaatgc cggccacgac cgaaacgcag
      541 gagatcgccg acaaggtgag cctgggccca ggtcaggcca gcaggaccca gacctacggg
      601 gaccgggcga cccaaaccga cccgggtcct gcagccggtt tcagatgcta ccgcaagtgt
      661 gactgggagc tgtgactctg ggcctggttg gaggattcag ggctggggtt ggggaagagt
      721 cttctgacgc ttgcaaacaa gaggttaact cacttcttag gaaactcagg gattggtgga
      781 gggatccggt agtcctgaag cattcaggaa ggcagctcac agtgttagaa tccaggctgt
      841 cccaaagtag agacatcttc ttgacttaaa cgcaggccta aacaagagaa agccagcctg
      901 ggcttcagtg ggagtcttct caaatgatta gtgtacagct cagtggcaga actcaaactt
      961 gcttgtgcct agtctgaaac cagatgtgaa accttgggtc cgggcagctc ttcctgtgaa
     1021 tattagcaga gattctgtac aaactaggga ggtccagggt attagcaaga gaaggaaagt
     1081 taaccatgtc ctctcttcag ctcccaagcc caggacccta tccactaagc cgagctgacc
     1141 ttgggaggtc tctgctctga cggaagtggc caaggaggag tgagtcagct tcatgagacc
     1201 ctgaaaatga ggaattgcta cagacacact ggccaggcaa gggaaccctg gccaagtacc
     1261 acgtcaaagc tcaggaggca gagacaggcg aatctctctg agtttgaagc cagcttggtc
     1321 tgcatagttc tgtgtcagcc atagttacac agcgagaccc tgtctcaaga aagaagaagc
     1381 tgagttctcc tttatcagtg cggacaacag cttacttacc atgtccccgt agtgaggaac
     1441 gctagttttt gagagctggc agggggttcc cgagaggggc atgaagtcac ctgtgggtga
     1501 gtctgagtcc ttccttctca tccactatac aggtgaagtc tcaacttgaa gagaaagcaa
     1561 atcagaagtt tgatgtcttt aaagccatat ccttcaggag acaggtagtg gccggcacca
     1621 acttcttcat caaggtgggt acttaataat agcttgagaa agactgggga caaagtctca
     1681 gagtagagca gaatgtcctg caacttcccc ccagagcgcc ccttaagagg acacaatcag
     1741 ggctggaggg atggctcagt ggttagagca ctgactgctc ttccagaggt cctgagttca
     1801 aatcccagca accacatggt ggctcataac catctgtaat gggatctgat gccctctttt
     1861 ggtgtatctg aagacagcta cagtgtactt atatataata aataaatctt taataaaaaa
     1921 aaaagagaga ggacaggtac aggttctgag aggacgaatt tggggtgctg gggttcccgg
     1981 ccttaaagga ggagataaat gttaccactg gcttagttgc tggtgccctg ttctgcctca
     2041 actttctaaa gctgggttaa gcctagggct ggaaccccac cgttccctca cgggcctctc
     2101 tccccccccc ccaggttgat gtcggcgaag aaaaatgtgt gcacttgagg gtgtttgaac
     2161 ccctccctca tgagaacaag cctttgacct tgtcctctta ccagaccgac aaagaaaagc
     2221 acgatgagct aacctacttc tgattctgca gcccctttgc caaatacttc acctttggaa
     2281 tccgtgtttg ggaccacgaa gtaaataccc ctctgtgagc agcttccttt gtgatgccca
     2341 aacggcgttg tattttgttt ctttccaaac aattattttc agaaaactgt ataaaactat
     2401 ctctctaaat atatattttt agagaccgta caaactgtct ccttagtttt gaattttttt
     2461 cctttgttta aaaaaaaaaa tcacagtcac actaggcggt ggtggcacgt gcctttaatc
     2521 ccagtattca ggaggttgag gctagcctgg gctacagagt gaattc
//