From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human neutrophil cytosol factor 1 (NCF-47k) protein, complete cds.
Date: 5 Nov 92 23:11:35 GMT
Distribution: bionet
Checksum: 62461 4

LOCUS       HUMNCF1A     1339 bp ss-mRNA            PRI       15-MAR-1990
DEFINITION  Human neutrophil cytosol factor 1 (NCF-47k) protein, complete cds.
ACCESSION   M25665 M26193
KEYWORDS    neutrophil cytosol factor 1.
SOURCE      Human promyelocytic leukemia HL60 cell line, cDNA to mRNA, clone
            8A.
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 1339)
  AUTHORS   Volpp,B.D., Nauseef,W.M. and Clark,R.A.
  TITLE     Cloning of the cDNA and functional expression of the 47 kiloDalton
            cytosolic component of the human neutrophil respiratory burst
            oxidase
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 86, 7195-7199 (1989)
  STANDARD  full automatic
REFERENCE   2  (bases 1 to 1339)
  AUTHORS   Volpp,B.D., Nauseef,W.M. and Clark,R.A.
  TITLE     Correction
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 86, 9563-9563 (1989)
  STANDARD  full automatic
REFERENCE   3  (bases 1 to 1339)
  AUTHORS   Lomax,K.J., Leto,T.L., Nunoi,H., Gallin,J.I. and Malech,H.L.
  TITLE     Recombinant 47-kD cytosol factor restores NADPH oxidase in chronic
            granulomatous disease
  JOURNAL   Science 245, 409-412 (1989)
  STANDARD  full automatic
COMMENT     [2]  revises [3],[1].
               Draft entry and computer-readable sequence for [3] kindly
            submitted by H.L.Malech, 21-JUN-1989, and [1] by B.D.Volpp,
            14-JUL-1989.
FEATURES             Location/Qualifiers
     mRNA            <1..1339
                     /gene="NCF1"
     CDS             13..1185
                     /product="neutrophil cytosol factor 1"
                     /codon_start=1
                     /translation="MGDTFIRHIALLGFEKRFVPSQHYVYMFLVKWQDLSEKVVYRRF
                     TEIYEFHKTLKEMFPIEAGAINPENRIIPHLPAPKWFDGQRAAENRQGTLTEYCSTLM
                     SLPTKISRCPHLLDFFKVRPDDLKLPTDNQTKKPETYLMPKDGKSTATDITGPIILQT
                     YRAIADYEKTSGSEMALSTGDVVEVVEKSESGWWFCQMKAKRGWIPASFLEPLDSPDE
                     TEDPEPNYAGEPYVAIKAYTAVEGDEVSLLEGEAVEVIHKLLDGWWVIRKDDVTGYFP
                     SMYLQKSGQDVSQAQRQIKRGAPPRRSSIRNAHSIHQRSRKRLSQDAYRRNSVRFLQQ
                     RRRQARPGPQSPGSPLEEERQTQRSKPQPAVPPRPSADLILNRCSESTKRKLASAV"
BASE COUNT      287 a    441 c    394 g    217 t
ORIGIN      
        1 ggccacccag tcatggggga caccttcatc cgtcacatcg ccctgctggg ctttgagaag
       61 cgcttcgtac ccagccagca ctatgtgtac atgttcctgg tgaaatggca ggacctgtcg
      121 gagaaggtgg tctaccggcg cttcaccgag atctacgagt tccataaaac cttaaaagaa
      181 atgttcccta ttgaggcagg ggcgatcaat ccagagaaca ggatcatccc ccacctccca
      241 gctcccaagt ggtttgacgg gcagcgggcc gccgagaacc gccagggcac acttaccgag
      301 tactgcagca cgctcatgag cctgcccacc aagatctccc gctgtcccca cctcctcgac
      361 ttcttcaagg tgcgccctga tgacctcaag ctccccacag acaaccagac aaaaaagcca
      421 gagacatact tgatgcccaa agatggcaag agtaccgcga cagacatcac cggccccatc
      481 atcctgcaga cgtaccgcgc cattgccgac tacgagaaga cctcgggctc cgagatggct
      541 ctgtccacgg gggacgtggt ggaggtcgtg gagaagagcg agagcggttg gtggttctgt
      601 cagatgaaag caaagcgagg ctggatccca gcatccttcc tcgagcccct ggacagtcct
      661 gacgagacgg aagaccctga gcccaactat gcaggtgagc catacgtcgc catcaaggcc
      721 tacactgctg tggaggggga cgaggtgtcc ctgctcgagg gtgaagctgt tgaggtcatt
      781 cacaagctcc tggacggctg gtgggtcatc aggaaagacg acgtcacagg ctactttccg
      841 tccatgtacc tgcaaaagtc ggggcaagac gtgtcccagg cccaacgcca gatcaagcgg
      901 ggggcgccgc cccgcaggtc gtccatccgc aacgcgcaca gcatccatca gcggtcgcgg
      961 aagcgcctca gccaggacgc ctatcgccgc aacagcgtcc gttttctgca gcagcgacgc
     1021 cgccaggcgc ggccgggacc gcagagcccc gggagcccgc tcgaggagga gcggcagacg
     1081 cagcgctcta aaccgcagcc ggcggtgccc ccgcggccga gcgccgacct catcctgaac
     1141 cgctgcagcg agagcaccaa gcggaagctg gcgtctgccg tctgaggctg gagcgcagtc
     1201 cccagctagc gtctcggccc ttgccgcccc gtgcctgtac atacgtgttc tatagagcct
     1261 ggcgtctgga cgccgagggc agccccgacc cctgtccagc gcggctcccg ccaccctcaa
     1321 taaatgttgc ttggagtgg
//