From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Klebsiella terrigena budABC operon, including alpha-acetolactate
Date: 21 Dec 92 20:48:00 GMT
Distribution: bionet
Checksum: 33654 8

LOCUS       KPNBUDOPRN   3574 bp ds-DNA             BCT       20-NOV-1992
DEFINITION  Klebsiella terrigena budABC operon, including alpha-acetolactate
            decarboxylase (budA), alpha-acetolactate synthase (budB), and
            acetoin(diacetyl)reductase (budC) genes, complete coding regions.
ACCESSION   L04507
KEYWORDS    acetoin(diacetyl)reductase; acetolactate decarboxylase;
            acetolactate synthase; budA gene; budABC operon; budB gene;
            budC gene.
SOURCE      Klebsiella terrigena (library: VTT-E-74023) DNA.
  ORGANISM  Klebsiella terrigena
            Prokaryotae; Gram-negative facultatively anaerobic rods;
            Enterobacteriaceae.
REFERENCE   1  (bases 1 to 3574)
  AUTHORS   Blomqvist,K., Nikkola,M., Lehtovaara,P., Suihko,M.-L.,
            Airaksinen,U., Straby,K.B., Knowles,J.K. and Penttilae,M.E.
  TITLE     Characterization of the genes of the 2.3-Butanediol operon from
            Klebsiella terrigena and Enterobacter aerogenes
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     mRNA            145..>3396
                     /note="budABC operon"
     RBS             166..170
                     /note="budABC operon"
     CDS             179..958
                     /EC_number="4.1.1.5"
                     /gene="budA"
                     /product="alpha-acetolactate decarboxylase"
                     /codon_start=1
                     /translation="MNHYPECTCQESLCETVRGFSAHHPDSVIYQTSLMSALLSGVYE
                     GSTTIADLLTHGDFGLGTFNELDGELIAFSSEVYQLRADGSARKARADQKTPFAVMTW
                     FRPQYRKTFDHPVSRQQLHDVIDQQIPSDNLFCALHIDGHFRHAHTRTVPRQTPPYRA
                     MTDVLDDQPVFRFNQRKGTLVGFRTPQHMQGLNVAGYHEHFITDDRQGGGHLLDYQLD
                     SGVLTFGEIHKLMIDLPADSAFLQADLHPDNLDAAIRAVEN"
     CDS             969..2648
                     /EC_number="4.1.3.18"
                     /gene="budB"
                     /product="alpha-acetolactate synthase"
                     /codon_start=1
                     /translation="MDKPRHERQWAHGADLIVSQLEAQGVRQVFGIPGAKIDKVFDSL
                     LDSSIRIIPVRHEANAAFMAAAVGRITGKAGVALVTSGPGCSNLITGMATANSEGDPV
                     VALGGAVKRADKAKLVHQSMDTVAMFSPVTKYAVEVTASDALAEVVSNAFRAAEQGRP
                     GSAFVSLPQDIVDGPASGSTLPASRAPQMGAAPDGAVDSVAQAIAAAKNPIFLLGLMA
                     SQPENSRALHRHAGKKPYSGHQHLSGAGAVNQDNFARFAGRVGLFNNQAGDRLLRQAD
                     LIICIGYSPVEYEPAMWNSGTATLVHIDVLPAYEERNYVPDIELVGDIAATLEKLAQR
                     IEHRLVLTPQAADILADRQRQRELLDRRGAQLNQFALHPLRIVRAMQDIVNSDVTLTV
                     DMGSFHIWIARYLYSFRARQVMISNGQQTMGVALPWAIGAWLVNPQRKVVSVSGDGGF
                     LQSSMELETAVRLHANILHIIWVDNGYNMVAIQEQKKYQRLSGVEFGPVDFKVYAEAF
                     GACGFAVESAEALEPTLRAAMDVDGPAVVAIPVDYRDNPLLMGQLHLSQIL"
     CDS             2671..3396
                     /gene="budC"
                     /product="acetoin(diacetyl)reductase"
                     /codon_start=1
                     /translation="MQKVALVTGAGQGIGKAIALRLVKDGFAVAIADYNDATATAVAA
                     EINQAGGRAVAIKVDVSRRDQVFAAVEQARKALGGFNVIVNNAGIAPSTPIESITEEI
                     VDRVYNINVKGVIWGMQAAVEAFKKEGHGGKIVNACSQAGHVGNPELAVYSSSKFAVR
                     GLTQTAARDLAPLGITVNGFCPGIVKTPMWAEIDRQCRKRRANRWATARLNLPNASPL
                     AACRSLKTSPPACRSSPARIPTI"
BASE COUNT      725 a   1118 c   1061 g    670 t
ORIGIN      
        1 atcgtgggcc gcccgagtga agttccgcgc ctcggcaacg gcgacaaaat aacgtaaata
       61 gcgaagttcc atatcgtaaa cgtctcaaac cagcatggat tctatattgg aactgagagc
      121 tgaatcgggt caatatttat ttaatctttc ttatatttgt tgaacgagga agtggattgt
      181 gaatcattat cctgaatgca cctgccagga gagcctgtgc gaaaccgtac gcggcttctc
      241 cgcccaccac cctgatagcg ttatctatca gacctctctg atgagcgcgc tgctgagcgg
      301 ggtctatgag ggtagcacca ccatcgccga cctgctgacc cacggcgact tcggtctcgg
      361 cacctttaac gaactcgatg gcgaactgat tgcctttagc agcgaggtct accagctgcg
      421 cgctgacggc agcgcgcgta aagcccgggc ggatcaaaaa acgcccttcg cggtgatgac
      481 ctggttcaga ccgcagtacc gtaaaacctt tgaccacccg gtcagccgcc agcagctgca
      541 cgacgttatc gaccagcaaa tcccctccga taacctgttc tgcgccctgc atattgatgg
      601 tcactttcgc cacgcccaca cccgcaccgt gccgcggcag acgccgccct atcgggcgat
      661 gaccgacgtg ctcgatgacc agccggtttt ccgcttcaac cagcgcaagg ggacgctggt
      721 cggctttcgc accccgcagc atatgcaggg ccttaacgtt gccggctacc acgagcactt
      781 tattaccgac gatcgccagg gcggcggcca tctgctggac taccagctcg atagcggcgt
      841 gctgaccttc ggcgagatcc acaagctgat gattgacctc ccggccgaca gcgctttcct
      901 gcaggccgac ctgcatcctg acaatctcga tgccgctatt cgtgcggtag aaaactaagg
      961 agcttcagat ggacaaaccg cgtcacgaac gtcaatgggc ccacggtgcc gacttaatcg
     1021 tcagccagct tgaggcccag ggcgtacgcc aggtcttcgg catccccggt gccaaaatcg
     1081 acaaggtgtt tgattccctc ctcgactcct caatccgcat tattccggtg cgccacgagg
     1141 ctaacgccgc ctttatggcc gcggcggtcg ggcggattac cggtaaagcg ggcgtcgcgc
     1201 tggtgacctc cggtcccggc tgctcaaacc tgattaccgg catggccacc gccaatagcg
     1261 aaggcgaccc ggtggtggcg ctgggcggcg cggtgaagcg cgcggataag gccaagctgg
     1321 ttcaccaaag catggacacc gtggcgatgt tcagcccggt caccaaatac gccgtcgagg
     1381 tgaccgcctc cgacgcgctg gccgaggtgg tctccaacgc ctttcgcgcc gccgaacagg
     1441 ggcgtccggg gagcgcgttt gtcagcctgc cgcaggatat cgttgacggc cccgccagcg
     1501 gcagcacgct gcccgccagc agagcgccgc agatgggcgc cgcgccggat ggcgccgttg
     1561 acagcgtggc gcaggcgatc gccgcggcga agaaccctat cttcctgctc gggctgatgg
     1621 ccagccagcc ggaaaacagc cgcgccctgc accgccatgc tggaaaaaag ccatattccg
     1681 gtcaccagca cctatcaggc gccggggcgg taaatcagga taacttcgcc cgcttcgccg
     1741 gccgggtagg cctgtttaat aaccaggcgg gcgatcgcct gctgcgtcag gcggacctga
     1801 tcatctgcat cggctatagc ccggttgagt acgaaccggc gatgtggaac agcggcacgg
     1861 caaccctggt gcatatcgac gtgctgccgg cctatgaaga gcggaactac gtcccggata
     1921 tcgagctggt gggcgacatc gccgccaccc tcgagaagct ggcccagcgc attgaacatc
     1981 ggctggtgtt aactccgcag gcggcggaca tcctcgccga ccgccagcgc cagcgggagc
     2041 tgcttgaccg ccgcggggcg cagctgaatc agtttgcgct ccacccgctg cgcatcgtgc
     2101 gggcgatgca ggatatcgtc aatagcgacg tcaccttgac cgtcgatatg ggcagtttcc
     2161 atatctggat tgcccgctac ctctacagct tccgcgcccg ccaggtgatg atctccaacg
     2221 gtcagcaaac gatgggcgtc gcgctgccgt gggcaatcgg cgcgtggctg gtcaatccgc
     2281 agcgcaaggt ggtctcggta tccggcgatg gcggcttcct gcagtcgagc atggagctgg
     2341 agaccgccgt gcgcctgcac gccaatattc tgcacatcat ctgggtcgat aacggctaca
     2401 acatggtggc gattcaggaa cagaagaaat atcagcgcct ctccggcgtg gagttcggcc
     2461 cggtcgattt caaagtctac gccgaagcgt tcggggcctg cgggtttgcg gtagagagcg
     2521 ccgaggccct ggagccgacc ctgcgcgcgg cgatggatgt cgacggcccg gcggtggtcg
     2581 ccattccggt cgattaccgc gataaccctc tgctgatggg ccagctccat ctcagccaaa
     2641 tactgtgagt cactacaaaa ggaaatgaaa atgcaaaaag tcgcacttgt caccggcgcc
     2701 ggtcagggca tcggtaaagc tatcgccctg cgtctggtga aggatggatt tgccgtggca
     2761 atcgccgatt acaacgacgc tacggccaca gcggtagccg ctgaaatcaa ccaggccggc
     2821 ggccgcgcgg tggccattaa ggtcgacgtc tcgcgccggg accaggtttt cgccgccgtt
     2881 gagcaggcgc gtaaagccct gggcggattc aacgttatcg tcaacaacgc cggcatcgcg
     2941 ccgtcaacgc cgatcgagtc catcaccgag gagatcgtcg accgggtcta taacatcaac
     3001 gttaagggcg tcatctgggg gatgcaggcg gcggtggagg ccttcaaaaa agaggggcac
     3061 ggcgggaaga tcgtcaacgc ctgctcccag gccggccacg tcggcaaccc ggagctggcg
     3121 gtctacagtt cgagtaaatt cgccgtgcgc ggcctgacgc aaaccgccgc ccgcgatctg
     3181 gcgccgctgg gcatcaccgt taacggcttc tgcccaggga tcgttaagac gccaatgtgg
     3241 gcggagattg accgtcagtg tcggaagcgg cgggcaaacc gctgggctac ggcacggctg
     3301 aatttgccaa acgcatcacc cttggccgcc tgtcggagcc tgaagacgtc gccgcctgcg
     3361 tgtcgttcct cgccagcccg gattccgact atatgaccgg ccaatcgctg ctgatcgacg
     3421 gcggcatggt attcaactaa tcgtagtatt taattaatcg tggtattcaa ctaataatat
     3481 taagctctga catgatttgc cctgatctaa cgcaggggcc tttttatgcc ctatcccacc
     3541 gccagtgcaa acaaatcagc ccttcacaat ctcc
//