From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human gene for mitochondrial acetoacetyl-CoA thiolase, exon 12.
Date: 24 Jul 92 10:06:58 GMT
Distribution: bionet
Checksum: 42381     4

LOCUS       HUMMAT12      409 bp ds-DNA             PRI       24-JUL-1992
DEFINITION  Human gene for mitochondrial acetoacetyl-CoA thiolase, exon 12.
ACCESSION   D10511 D90487
KEYWORDS    acetoacetyl-CoA thiolase; mitochondria;
            mitochondrial acetoacetyl-CoA thiolase.
SEGMENT     12 of 12
SOURCE      Homo sapiens (individual_isolate control Japanese)(library: lEMBL3
            human genomic library) peripheral leukocytes DNA, clones
            lHT2K[1,2,3,6,7,8,9].
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (sites)
  AUTHORS   Fukao,T., Yamaguchi,S., Kano,M., Orii,T., Fujiki,Y., Osumi,T. and
            Hashimoto,T.
  TITLE     Molecular cloning and sequence of the complementary DNA encoding
            human mitochondrial acetoacetyl-coenzyme A thiolase and study of
            the variant enzymes in cultured fibroblasts from patients with
            3-Ketothiolase deficiency
  JOURNAL   J. Clin. Invest. 86, 2086-2092 (1990)
  STANDARD  full staff_review
REFERENCE   2  (bases 1 to 409)
  AUTHORS   Kano,M., Fukao,T., Yamaguchi,S., Orii,T., Osumi,T. and Hashimoto,T.
  TITLE     Structure and expression of the human mitochondrial acetoacetyl-coA
            thiolase gene
  JOURNAL   Unpublished (1992)
  STANDARD  full staff_review
COMMENT     Data kindly submitted in computer readable form by:
            Masatsugu Kano
            Department of Pediatrics
            Gifu University
            School of Medicine
            Gifu 500
            Japan
            Phone:  0582-65-1241 x 2819
            Fax:    0582-65-9011
            [1]  sites.
FEATURES             Location/Qualifiers
     CDS             join(D10500:989..1060,D10501:61..108,D10502:61..178,
                     D10503:61..156,D10504:61..161,D10505:61..204,
                     D10506:61..211,D10507:61..156,D10508:61..174,
                     D10509:61..125,D10510:61..218,61..181)
                     /EC_number="2.3.1.9"
                     /product="mitochondrial acetoacetyl-CoA thiolase"
                     /gene="ACAT"
                     /codon_start=1
                     /translation="MAVLAALLRSGARSRSPLLRRLVQEIRYVERSYVSKPTLKEVVI
                     VSATRTPIGSFLGSLSLLPATKLGSIAIQGAIEKAGIPKEEVKEAYMGNVLQGGEGQA
                     PTRQAVLGAGLPISTPCTTINKVCASGMKAIMMASQSLMCGHQDVMVAGGMESMSNVP
                     YVMNRGSTPYGGVKLEDLIVKDGLTDVYNKIHMGSCAENTAKKLNIARNEQDAYAINS
                     YTRSKAAWEAGKFGNEVIPVTVTVKGQPDVVVKEDEEYKRVDFSKVPKLKTVFQKENG
                     TVTAANASTLNDGAAALVLMTADAAKRLNVTPLARIVAFADAAVEPIDFPIAPVYAAS
                     MVLKDMGLKKENIAMWEVNEAFSLVVLANIKMLEIDPQKVNINGGSVSLGHPIGMSGA
                     RIVGHLTHALKQGEYGLASFCNGGGGASAMLIQKL"
     exon            61..358
                     /number=12
                     /product="mitochondrial acetoacetyl-CoA thiolase"
                     /gene="ACAT"
     polyA_signal    353..358
                     /gene="ACAT"
BASE COUNT      118 a     64 c     77 g    150 t
ORIGIN
        1 attttggtta gtcataaatt ctgtacttca ttaaagaagt aaatgctttc ttaattttag
       61 gatgtctgga gccaggattg ttggtcattt gactcatgcc ttgaagcaag gagaatacgg
      121 tcttgccagt ttttgcaatg gaggaggagg tgcttctgcc atgctaattc agaagctgta
      181 gacaacctct gctatttaag gagacaaccc tatgtgacca gaaggcctgc tgtaatcagt
      241 gtgactactg tgggtcagct tatattcaga taagctgttt cattttttat tattttctat
      301 gttaactttt aaaaatcaaa atgatgaaat cccaaaacat tttgaaatta aaaataaatt
      361 tcttcttctg cttttttctt ggtaaccttg aaaagtttga tacattttt
//