From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Erwina herbicola pheA, tyrA, and aroF genes
Date: 29 Dec 92 00:50:21 GMT
Distribution: bionet
Checksum: 05238 8

LOCUS       ERWPHEAGN    3434 bp ds-DNA             BCT       04-DEC-1992
DEFINITION  Erwina herbicola pheA, tyrA, and aroF genes
ACCESSION   X60420
KEYWORDS    DAHP synthase; P-protein; T-protein; aroF gene;
            bifunctional protein; chorismate mutase/prephenate dehydratase;
            pheA gene; tyrA gene; tyrosine-sensitive DAHP synthase isoenzyme.
SOURCE      Erwinia herbicola DNA.
  ORGANISM  Erwinia herbicola
            Prokaryotae; Gram-negative facultatively anaerobic rods;
            Enterobacteriaceae.
REFERENCE   1  (bases 1 to 3434)
  AUTHORS   Xia,T.H., Zhao,G. and Jensen,R.A.
  TITLE     A monofunctional prephenate dehydrogenase created by cleavage of
            the 5' 109 bp of the tyrA gene from Erwinia herbicola
  JOURNAL   J. Gen. Microbiol. 138, 1309-1316 (1992)
  STANDARD  full automatic
COMMENT     *source: clone_library=Sau 3A:pUC18; *source: clone=pJX181;
               From EMBL    entry EHPHEAGN;  dated 27-OCT-1992.
FEATURES             Location/Qualifiers
     promoter        243..272
                     /gene="pheA"
     terminator      395..416
                     /note="pheA attenuator terminator"
     terminator      1616..1645
                     /gene="pheA"
     terminator      complement(1616..1645)
                     /gene="tyrA"
     CDS             445..1608
                     /EC_number="4.2.1.51"
                     /EC_number="5.4.99.5"
                     /gene="pheA"
                     /product="prephenatedehydratase"
                     /codon_start=1
                     /translation="MNPDNPLLALRDKISAVDKKLLTLLAERRLLAVEVAQAKLATHR
                     PIRDVERERALLENLIVLGKAHNLDAHYITRLFQLVIEDSVLTQQALLQKNLNHPHAH
                     AARIAFLGPKGSYSHLAARNYASRHFDSMVECGCLKFHDIIKQVENGVADYAVMPIEN
                     TSSGSINDVYDLLQQTSLSIVGELTLPIDHCVLVNGPTDLQQIETVYSHPQPFQQCSQ
                     FINRFPHWKIEYTESTAAAMEKVAALNSPKVAALGSEAGGELYQLQVLERNLANQQQN
                     HTRFIVLARKAIEVSDQVPAKTTLIMATGQQAGALVDALLVLRQHNLIMSKLESRPIN
                     GNPWEEMFYIDVQGNLQSERMQQALQELQTMTRSLKVLGCYPSENVVPAEPGR"
     CDS             complement(1665..2786)
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /gene="tyrA"
                     /product="prephenatedehydrogenase"
                     /codon_start=1
                     /translation="MVAELTALRDQIDSVDKALLDLLAKRLELVAEVGEVKSRYGLPI
                     YVPEREASMLASRRKEAEALGVPPDLIEDVLRRVMRESYTSENDKGFKTLCPELRPVV
                     IVGGKGQMGRLFEKMLGLSGYTVKTLDKEDWPQAETLLSDAGMVIISVPIHLTEQVIA
                     QLPPLPEDCILVDLASVKNRPLQAMLAAHNGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     QPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVNLDQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSESNLALIKRYYQRFGEAIALLEQG
                     DKQAFIASFNRVEQWFGDHAKRFLVESRSLLRSANDSRP"
     CDS             complement(2792..>3434)
                     /EC_number="4.1.2.15"
                     /EC_number="5.4.99.5"
                     /gene="aroF"
                     /product="tyr-sensitive DAHP synthase isoenzyme"
                     /codon_start=2
                     /translation="DPNSPQYLGDLFSWSAIGARTTESQTHREMASGLSMPVGFKNGT
                     DGSLGTAINAMRAAAMPHRFVGINQAGQVCLLQTQGNPDGHVILRGGKAPNYGPEDVA
                     QCEKEMLKAGLRPALMIDCSHGNSNKDYSRQPGVAESAIAQIKDGNRSIIGLMLESHI
                     NEGNQSSEQPRSEMKYGVSVTDACINWEVTETLLREMHQDLQGVLSARLSQEV"
BASE COUNT      793 a    988 c    881 g    772 t
ORIGIN      
        1 gaattcgtgg ctgacgccac tattaacaca cctaacggga ctctggtggc cagcgccaag
       61 cacgatgata tgtataccgc tatcaacgat ttaattgcca agctcgagcg tcagctgaat
      121 aaagttcagc acaagtctga agcacgccgc gccagtgcca gcgtgaaaga catcattccc
      181 gccgacgaag agtcctgatt ttcttaacgc tgtttcttaa cgcgcctgcg ggcgggtttt
      241 tattgacagc ttttaagcag tacggttact ctcaggtcac tctcttttcg gacatgacaa
      301 tgaaacctga tctgtttttc ttcgctttct tttttacctt cccctgaacg ggaggccatt
      361 cgtcgtgtga aaatgaaagc gaagacgaac accgaagcct cccaaccggg aggctttttt
      421 ttggatagca tacaggtgag ccctatgaat cccgataatc cattgctggc gctacgcgac
      481 aaaatcagcg ccgtggataa aaaacttctg acgctgctgg ccgagcgtcg tttgctggca
      541 gtggaagttg cacaagcgaa actggcgacg catcgcccga ttcgggatgt ggagcgcgaa
      601 cgcgcgctac tggagaatct catcgtactg ggtaaggcac acaacctgga tgcgcactac
      661 atcacccgtc tgttccagct ggtaattgaa gattccgtgc tgactcagca agcgctgctg
      721 caaaaaaacc tcaaccatcc ccatgcgcat gccgcccgta tcgcctttct gggcccgaaa
      781 ggctcttatt cgcatcttgc agcacgcaac tacgcgtcgc gccatttcga tagcatggtg
      841 gagtgtggct gcctgaagtt tcacgacatc atcaaacagg tcgaaaatgg cgtagctgat
      901 tacgccgtga tgccgattga gaataccagt tcaggctcga tcaacgatgt ctacgatctg
      961 ctgcaacaga ccagcctctc aatcgtgggc gagctgacat tgcctatcga ccactgcgtg
     1021 ctggtgaatg gccccaccga tcttcagcag attgagacgg tttacagcca tcctcaaccg
     1081 ttccagcagt gcagccagtt tattaatcgt ttcccgcact ggaaaatcga atacaccgag
     1141 agtaccgcgg cagcgatgga gaaagtggct gcgctcaact cacctaaggt ggcggcgctg
     1201 ggcagtgagg ccggcggcga actgtatcag ctgcaggtgc tggaacgtaa tctggcgaat
     1261 cagcagcaga accacacccg ctttatcgtg ctggcccgca aagcaatcga ggtttctgac
     1321 caggtgccag cgaaaaccac gctgattatg gcaaccggcc agcaggcggg tgcgctggtc
     1381 gacgcgctgc tggtactgcg tcagcacaac ctgattatga gcaagctgga gtcccgcccg
     1441 attaatggta atccgtggga agagatgttt tacattgatg tgcagggcaa cctgcaatca
     1501 gagcggatgc agcaggcgct gcaggaatta cagaccatga cccgctctct gaaggtactg
     1561 ggctgttacc ccagcgaaaa tgtggtgccc gctgaacctg gccggtaaaa agaagaaaaa
     1621 aggcatccaa ctggatgcct tttttattga tggatggctg tcatttatgg gcggctgtca
     1681 ttggccgatc gcaacaggct tcggctttcg accaggaagc gttttgcgtg atcgccaaac
     1741 cactgttcaa cccggttaaa gctggcgata aacgcctgct tgtcgccctg ctccagcagc
     1801 gcaatcgctt caccaaaccg ctggtaatag cgttttatca gcgccagatt actctctgaa
     1861 gacatgatga tatccgcata gagttgcgga tcctgagcga acaaccgccc caccatcgcc
     1921 agttcaagcc ggtaaatggg cgacgagagc gccagcagct gatccagatt gacgttctct
     1981 tcggctaaat gcagaccata agcgaaggta gcaaagtgac gcagcgcctg aatgaatgcc
     2041 atgttctggt catgctcaac agcgctgata cgatgcagac gcgcacccca gacctgaatc
     2101 tgctccagga accactgata cgcttccggt tgtcttccat cacaccagac caccacctgt
     2161 tttgccaggc tgccgctgtc cgggccaaac atcggatgca gacccagtac aggcccgtta
     2221 tgggcagcca gcattgcctg cagaggccgg tttttgactg acgccagatc gaccagaata
     2281 caatcttccg gcagtggtgg cagttgggca atcacctgct cggtcaggtg aatcggcacg
     2341 ctaatgatca ccattccggc atcgctgagc agagtctcag cctgaggcca gtcctcttta
     2401 tccagcgttt taaccgtgta gcctgatagc ccgagcattt tttcaaacag ccggcccatc
     2461 tggcccttac caccgacaat caccaccggg cgcagttcag gacagagggt tttaaagcct
     2521 ttatcattct cgctggtata ggattcccgc atcacgcgac gcagcacatc ctcaatcaga
     2581 tccggtggta cgccgagcgc ttcggcctct ttgcgacgcg aagccagcat cgacgcctca
     2641 cgctcaggca catagatagg caggccgtaa cggctcttca cctcacctac ctcggccacc
     2701 agttccagtc gcttagccag cagatccagc agcgctttat ctacactgtc aatttgatcg
     2761 cgtaacgcgg tcagttcagc caccattgct cttacacctc ttgtgacaga cgcgccgaca
     2821 gcactccctg cagatcctga tgcatttcac gcagcaacgt ttcggtcact tcccagttga
     2881 tgcatgcatc ggttacggaa acgccatact tcatttcgct gcgtggctgc tcagaagact
     2941 ggttaccttc attgatatgg ctttccagca tcaagccaat gattgaacgg ttcccgtctt
     3001 tgatctgcgc aatagcggat tccgctacgc caggctgacg gctgtagtct ttgttcgaat
     3061 taccatggct gcaatctatc attaaggctg gacgcagtcc cgccttgagc atctcttttt
     3121 cacactgcgc gacatcttca ggaccgtagt tcggcgcttt gccgccacgc aggatcacgt
     3181 gaccatccgg attgccctga gtctgcagca ggcagacctg accggcctga ttaatcccga
     3241 caaaacggtg tggcattgcg gcagcacgca tggcgttgat tgccgttccc agactgccat
     3301 cggtgccatt tttaaagcct accggcatcg acaggcccga ggccatttca cggtgcgtct
     3361 gcgattcagt ggtacgggcg ccaatggcag accagctgaa caggtcgccc agatattgcg
     3421 gactattggg atcc
//