From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: orf2...orf1 [Selenomonas ruminantium, pJDB23, lactilytica, Plasmid
Date: 29 Dec 92 19:51:24 GMT
Distribution: bionet
Checksum: 12856 8

LOCUS       S116042      3759 bp    DNA   Circular  NLM       09-DEC-1992
DEFINITION  orf2...orf1 [Selenomonas ruminantium, pJDB23, lactilytica, Plasmid
            Complete, 3 genes, 3759 nt]
ACCESSION   S46709 M86247
KEYWORDS    .
SOURCE      Selenomonas ruminantium pJDB23 lactilytica
  ORGANISM  Selenomonas ruminantium
            Unclassified.
REFERENCE   1  (bases 1 to 3759)
  AUTHORS   Attwood,G.T. and Brooker,J.D.
  TITLE     Complete nucleotide sequence of a Selenomonas ruminantium plasmid
            and definition of a region necessary for its replication in
            Escherichia coli.
  JOURNAL   Plasmid 28, 123-129 (1992)
  STANDARD  full automatic
COMMENT     This entry [NCBI gibbsq 116042] was created by the journal scanning
            component of NCBI/GenBank at the National Library of Medicine.
            This sequence comes from Fig. 1.
FEATURES             Location/Qualifiers
     CDS             join(3652..3759,1..696)
                     /note="Description: orf2;  For the protein sequence (NCBI
                     gibbsq 116051):  Method: conceptual translation supplied
                     by author.  This sequence comes from Fig. 1."
                     /codon_start=1
                     /translation="MDIQSIQQSQAIQLTLGQATKTTKATNGSFLTMFNSLQGSSSQL
                     ADMWKSSFEKNFSQTTSQYFYHVMDVPKISDTTWEHNDFPYDKLTQENIDSNVFSWQP
                     TRGNPNQLDAKVQAKNQAMLGKNTIIVPPALEEKMQQNPKLAQKVAANIERIYEIHRP
                     MPHLPMPGTKFYGTKMYGSVIILNEDGDVEHSCVTSGGGIVGPDEKTLRQFEGQQRKK
                     QKRREEKQHLAQEATIAHIDQHIDMMRFLQHQKATYEFLRTVLNLPCWQ"
     CDS             complement(1126..1674)
                     /note="Description: orf3;  For the protein sequence (NCBI
                     gibbsq 116053):  Method: conceptual translation supplied
                     by author.  This sequence comes from Fig. 1."
                     /codon_start=1
                     /translation="MTFLELQNQIVQTRKARKGIKNREDYFQDDETEEFWTSVYKRAL
                     YIANEVVKHYEKKGAYDSSEKFHLSWGEAPNDYFDDYIKQMKAYEKEVKDYQKLWKEH
                     SELFDGKLKSDKEIAQLKRELSAAKAELVRVKRGEDTKIMRKSIKWSIIKKRIHQQLF
                     ERLPRLWGFLQQQFRKHWWQMV"
     CDS             complement(2203..3114)
                     /note="mismatch(205[S->T]).  Description: orf1;  For the
                     protein sequence (NCBI gibbsq 116059):  Method: conceptual
                     translation supplied by author.  This sequence comes from
                     Fig. 1.
                     Author-given protein sequence is in conflict with the
                     conceptual translation"
                     /codon_start=1
                     /translation="MAKKPTDKQLFKMKNEWMGQFYERVSPRPFYRDVFPEGSFERKG
                     CPEDSKSNGVVTVIDGEKPRNYLVFDDLETIDDIKGTEFAIMSPVGYSGRNRTAKNAR
                     WLYGIGIDLDGVGMAQLRDVFHQMKHEFLPQCTYCVNSGHGLHLYYLLEKPVPLYRHL
                     HDRLRDFKYELIAKIWNRYTSTYTERDQVQYQGIFQGFRAVGTQSKLGKRYIVTAFKT
                     GERVTIEYLNTFLMDKSKALTDFGYKSALELSAAKEKYPEWYERRVVRGEDRGRWHIK
                     RDLYDWWLRKLKGAKPPWGIGTGACLC"
BASE COUNT     1071 a   1004 c    646 g   1038 t
ORIGIN      
        1 ctgcagggat catcttccca gttggcagat atgtggaaaa gctcttttga aaaaaacttt
       61 tcccaaacca ccagccaata tttttatcat gtcatggatg taccaaagat ttctgacacc
      121 acatgggaac ataatgactt tccctatgat aagctgacac aggaaaatat cgacagcaac
      181 gtattcagtt ggcaaccaac acgggggaac ccaaaccaac ttgacgccaa ggtacaagct
      241 aaaaatcagg ccatgttagg aaaaaatacc atcattgttc cccccgcact agaagaaaaa
      301 atgcagcaaa atccaaagct ggcgcaaaaa gtggctgcaa atattgaacg gatatacgaa
      361 atacatcgcc caatgcctca tcttcccatg cccggcacaa aattctacgg cactaaaatg
      421 tatggctccg tgattatctt gaacgaagat ggcgatgtag aacactcctg tgttaccagt
      481 ggtggcggaa ttgtcggccc ggacgaaaaa acattacgtc agtttgaagg ccaacagcgc
      541 aaaaaacaaa aacgtcgaga ggaaaagcaa catttagctc aagaagccac cattgcacat
      601 attgaccagc atatagacat gatgcgattt ctgcaacacc aaaaggccac ttatgagttt
      661 ctccgaaccg tgcttaatct gccatgctgg cagtgaattt gcaccctacg gcttactgga
      721 ttttagtaat ccatttaccg cctggacggc ctaagcactc gtgtctcaat cgggagaatt
      781 gagacactca taagtgcgct tcggaaacca ccatcctgta ttacaaaata atttatacaa
      841 caaacggcac atagcctaca aaaaaagcaa cgcagttcag ggaggaaacg accggcaaaa
      901 tccccgaagg gtgcattgag tttcacagaa actcctagat gcacatgtaa cggttctaca
      961 cggcttacat gaaagcatct aggcaagatc cccgaagggg tgcagcgagt tttgcagaaa
     1021 ctcctaactg cacatggaag ccgttcctca cggctcccat ggaacgtaag caaaaaaagc
     1081 gaagatttct ccggcttgga cttgtcattt atgctttttg tttgttcaaa ccatttgcca
     1141 ccagtgcttt ctgaactgtt gttgtagaaa tccccaaagc cttggcaatc tctcgaacag
     1201 ttgctgatgg attctctttt ttataattga ccactttatg ctctttcgca taatctttgt
     1261 atcttcgcca cgcttcactc taaccagttc ggctttggct gctgacaatt ctcgctttaa
     1321 ttgagctatc tccttatccg acttcaattt tccatcaaaa agctcactat gttctttcca
     1381 aagcttttga taatctttaa cctctttctc atacgctttc atctgtttga tataatcatc
     1441 aaaataatca tttggagcct ctccccaaga tagatggaac ttttcagaag aatcgtaagc
     1501 cccctttttt tcatagtgct taacgacctc attagctata tacaacgccc tcttataaac
     1561 acttgtccaa aattcttccg tctcatcatc ttgaaaatag tcttccctat tctttatccc
     1621 tttacgcgcc ttgcgtgtct ggactatctg attctgtaat tccaaaaatg tcatagtgaa
     1681 caccgccttt gtattataaa gtaatttcta atacaattat agcagactga ttttgtgtta
     1741 acaagtattt gttaacacaa aatcagtctg aaaaattatt gccaccactt caagacagta
     1801 tgcctagaca agcccgttga gcgctcacaa tctatcttct tgccatctgg atgcgcagct
     1861 tgccattcta aaacaatctg ctgcttactc ggccgtccat ccctgttacg ccatgcccca
     1921 ttggataatc tacttcttgt actgcccttg cacgaactaa gtgtaaggcc tgctttctgc
     1981 cgttcctctt gttagctgga acggatatgc cactcacacg ctctacttac gccctgctat
     2041 acgttacgta actttcttga tacatattca tggcatccaa cacatcacgc tttgtaaagc
     2101 gattatgctc atcatcgcta atttcatcca gagacggcaa tagctccaaa gcatccgtta
     2161 gcacctcatc ttctggaaca tcacacttca ctccatacgc agctaacaca gacaagcacc
     2221 agtacctatg ccccacggag gcttcgcacc tttcagcttc cgaagccacc aatcatataa
     2281 atcacgcttg atatgccatc tgcctcggtc ttcacctctc acaacccgtc tttcatacca
     2341 ctctggatat ttttccttgg ctgccgaaag ttccaaagcc gatttatagc caaaatctgt
     2401 taaggccttg ctcttatcca tgaggaatgt gttcagatac tcaatagtaa cccgctcacc
     2461 agtcttaaag gctgtcacta tgtacctctt tcctagcttg gtctgtgtgc ctaccgcacg
     2521 aaacccctga aaaatccctt gatattgaac ctgatcacgc tctgtatagg tactggtata
     2581 tctgttccat atcttggcta tcagctcata cttaaaatcc cgcaacctgt catgcaaatg
     2641 cctatacaat ggcacaggct tctccagcaa gtagtataaa tgcaagccat gcccactatt
     2701 tacgcaatag gtacactggg gaagaaactc atgcttcatc tggtgaaaca catctcgcaa
     2761 ctgcgccata ccaaccccat caaggtcaat ccctatccca tatagccacc ttgcattttt
     2821 tgccgttcta ttacgcccag aatagcccac aggcgacatt atagcaaact ctgtaccctt
     2881 tatatcatca atggtctcta agtcatcaaa aacgagataa ttcctcggct tctccccatc
     2941 aatgacagta acaacaccat tactctttga atcctccggg cagcctttcc gctcaaaact
     3001 tccttctgga aacacatccc tataaaacgg cctcggggag accctttcgt agaactgccc
     3061 catccattca ttcttcatct tgaacaactg cttatccgtc ggtttttttg ccatatcaaa
     3121 atcccccttc accaccccct aaaaccctat atagggggct tatattatca atactgtttt
     3181 tgcacatttt tggattgcta aaaaaatccc ctcaccaccc ccctaaaacc ctatataggg
     3241 ggcttatatt atcaatactg tttttgcaca tttttggatt gaagaaacgc cagcctcatg
     3301 ctatactaat catacggccg atggtcggtt ccccgttcac gaggtgatgc catgactact
     3361 tacgagaagt tagcacttgt cgtggctttc cttcagttgg ttgtgacgct cctagctctg
     3421 cttaaataag catctaacgg tttaactacg ctttgacgaa cgtcccaagt ttttaacgaa
     3481 cggcggttcc gacgggctaa catcggccgc ttttcatgcc ttaattttac cacgccttgc
     3541 ctacacaggc aaggacttat ttatccagcg tcaactttaa gtctcccccg ccttttaccg
     3601 atataatcca taagaccgat ttatcaaagg cgaaaaggag gacaaagtgt catggacata
     3661 caaagcatcc agcaaagtca agctatacag ctaaccttag gacaagcaac aaaaaccaca
     3721 aaagccacta acggctcctt cctgacgatg tttaactcc
//