From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Newcastle disease virus fusion protein-F1 and fusion protein-F2
Date: 9 Apr 92 10:04:30 GMT
Distribution: bionet
Checksum: 10808     8

LOCUS       NDVFB        1759 bp ss-RNA             VRL       09-APR-1992
DEFINITION  Newcastle disease virus fusion protein-F1 and fusion protein-F2
            (F0) mRNA, complete cds.
ACCESSION   M21881
KEYWORDS    amphipathic alpha-helix; fusion protein; glycoprotein;
            integral membrane glycoprotein; transmembrane protein.
SOURCE      Newcastle disease virus (Australia-Victoria strain) from infected
            Chinese hamster ovary cells, cDNA to viral RNA, clones
            pTL[729,712,438,53].
  ORGANISM  Newcastle disease virus
            Viridae; ss-RNA enveloped viruses; Negative strand RNA viruses;
            Paramyxoviridae; Paramyxovirus.
REFERENCE   1  (bases 1 to 1759)
  AUTHORS   McGinnes,L.W. and Morrison,T.G.
  TITLE     Nucleotide sequence of the gene encoding the Newcastle disease
            virus fusion protein and comparisons of paramyxovirus fusion
            protein sequences
  JOURNAL   Virus Res. 5, 343-356 (1986)
  STANDARD  full staff_entry
REFERENCE   2  (sites)
  AUTHORS   Wang,C., Raghu,G., Morrison,T. and Peeples,M.E.
  TITLE     Intracellular processing of the Paramyxovirus F protein: Critical
            role of the predicted amphipathic alpha helix adjacent to the
            fusion domain
  JOURNAL   Unpublished (1991)
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     CDS             47..1708
                     /product="fusion protein precursor"
                     /gene="F0"
                     /codon_start=1
                     /translation="MGPRSSTRIPIPLMLTIRIALALSCVHLASSLDGRPLAAAGIVV
                     TGDKAVNIYTSSQTGSIIVKLLPNMPKDKEACAKAPLEAYNRTLTTLLTPLGDSIRRI
                     QESVTTSGGRRQKRFIGAIIGSVALGVATAAQITAASALIQANQNAANILRLKESITA
                     TNEAVHEVTDGLSQLAVAVGKMQQFVNDQFNNTAQELDCIKITQQVGVELNLYLTELT
                     TVFGPQITSPALTQLTIQALYNLAGGNMDYLLTKLGVGNNQLSSLIGSGLITGNPILY
                     DSQTQLLGIQVTLPSVGNLNNMRATYLETLSVSTTKGFASALVPKVVTQVGSVIEELD
                     TSYCIETDLDLYCTRIVTFPMSPGIYSCLNGNTSACMYSKTEGALTTPYMTLKGSVIA
                     NCKMTTCRCADPPGIISQNYGEAVSLIDRHSCNVLSLDGITLRLSGEFDATYQKNISI
                     LDSQVIVTGNLDISTELGNVNNSISNALDKLEESNSKLDKVNVKLTSTSALITYIALT
                     AISLVCGILSLVLACYLMYKQKAQQKTLLWLGNNTLGQMRATTKM"
     mat_peptide     47..442
                     /note="gene cleavage product 2"
                     /product="fusion protein-F2"
                     /gene="F0"
                     /codon_start=1
     mutation        224
                     /note="replace (224,`a`)"
                     /note="revertant D1R3, derived from D1"
                     /evidence=EXPERIMENTAL
                     /citation=[2]
     mutation        227
                     /note="replace (227,`c`)"
                     /note="revertant D1R7, derived from D1"
                     /evidence=EXPERIMENTAL
                     /citation=[2]
     mat_peptide     443..1705
                     /note="gene cleavage product 1"
                     /product="fusion protein-F1"
                     /gene="F0"
                     /codon_start=1
     mutation        504
                     /note="replace (504,`t`)"
                     /note="ts mutant D1 and revertants D1R3, D1R6, D1R7, D1R8
                     derived from D1"
                     /evidence=EXPERIMENTAL
                     /citation=[2]
     mutation        515
                     /note="replace (515,`g`)"
                     /note="ts mutant D2 and revertants D2R2 and D2R3 derived
                     from D2"
                     /evidence=EXPERIMENTAL
                     /citation=[2]
     mutation        515
                     /note="replace (515,`g`)"
                     /note="ts mutant D3 and revertants D3R1, D3R2 and D3R3
                     derived from D3"
                     /evidence=EXPERIMENTAL
                     /citation=[2]
     mutation        521
                     /note="replace (521,`g`)"
                     /note="revertants D1R6 and D1R8 derived from D1"
                     /evidence=EXPERIMENTAL
                     /citation=[2]
     mutation        521
                     /note="replace (521,`g`)"
                     /note="revertant D2R2, derived from D2"
                     /evidence=EXPERIMENTAL
                     /citation=[2]
     mutation        521
                     /note="replace (521,`g`)"
                     /note="revertants D3R1, D3R2, D3R3, derived from D3"
                     /evidence=EXPERIMENTAL
                     /citation=[2]
     conflict        709
                     /note="replace (709,'t')"
                     /note="J. Virol reported difference from Virus Res. 5,
                     343-356 (1986)"
                     /evidence=EXPERIMENTAL
                     /citation=[2]
                     /citation=[1]
     mutation        861
                     /note="replace (861,`t`)"
                     /note="revertant D2R3, derived from D2"
                     /evidence=EXPERIMENTAL
                     /citation=[2]
     mutation        895
                     /note="replace (895,`a`)"
                     /note="revertant D3R3, derived from D3"
                     /evidence=EXPERIMENTAL
                     /citation=[2]
     conflict        925
                     /note="replace (925,'g')"
                     /note="J. Virol reported difference from Virus Res. 5,
                     343-356 (1986)"
                     /evidence=EXPERIMENTAL
                     /citation=[2]
                     /citation=[1]
     mutation        1031
                     /note="replace (1031,`a`)"
                     /note="revertant D1R8, derived from D1"
                     /evidence=EXPERIMENTAL
                     /citation=[2]
BASE COUNT      515 a    402 c    397 g    445 t
ORIGIN      1 bp upstream of BamHI site.
        1 acgggtagaa gagtttggat cccggttggc gcattcaagg cgcaagatgg gccccagatc
       61 ttctaccagg atcccaatac ctctgatgtt gaccatccgg atcgcgctgg cactgagttg
      121 tgtccatctg gcaagttctc ttgatggcag gcctcttgca gctgcaggga tcgtggtaac
      181 aggggataaa gcagtcaaca tatacacctc atcccagaca gggtcaatca tagtcaagtt
      241 actcccaaat atgcccaagg ataaagaggc gtgtgcaaaa gccccgttgg aggcatacaa
      301 caggacactg actactttgc tcacccccct tggtgattct atccgcagga tacaagagtc
      361 tgtgactaca tccggaggaa ggagacagaa acgctttata ggtgctatta tcggcagtgt
      421 agctcttggg gttgcaacag ctgcacagat aacagcagcc tcggccctga tacaagccaa
      481 tcagaatgct gccaacatcc tccggcttaa ggagagcatt actgcaacca atgaagctgt
      541 acatgaggtc actgacggat tatcacaact agcagtggca gttgggaaga tgcagcagtt
      601 tgttaatgac cagtttaata acacagctca ggaattggac tgtataaaaa ttacacagca
      661 ggttggtgta gaactcaacc tgtacctaac tgaattgact acagtattcg ggccacaaat
      721 aacttcccct gccttaactc agctgactat ccaggcgctt tacaacctag ctggtggtaa
      781 tatggattac ttgttgacta agttaggtgt agggaacaac caactcagct cattaattgg
      841 tagcggcttg atcaccggca accctattct gtacgactca cagactcaac tcttgggtat
      901 acaggtaact ttaccctcag tcggaaacct aaataatatg cgtgccacct acctggagac
      961 cttgtctgta agcacaacca agggatttgc ctcagcactc gtcccaaaag tggtgacaca
     1021 ggtcggttct gtgatagaag aacttgacac ttcatactgt atagagaccg atttggattt
     1081 gtattgtaca agaatagtga cattccctat gtctcctggt atttattcct gtttgaacgg
     1141 caatacatcg gcttgcatgt attcaaagac tgaaggcgca cttactacgc catacatgac
     1201 tctcaaaggc tcagttattg ccaattgcaa gatgacaaca tgcagatgtg cagacccccc
     1261 gggtatcata tcgcaaaatt atggagaagc tgtgtctcta atagataggc actcatgcaa
     1321 tgtcttatcc ttggacggga taactttgag gctcagtggg gaatttgatg caacttatca
     1381 aaagaatatc tcaatactag attctcaagt aatagtgaca ggcaatctcg atatctcaac
     1441 tgagcttggg aatgtcaaca actcgataag taatgctttg gataagttag aggaaagcaa
     1501 cagcaaacta gacaaagtca atgtcaaact gaccagcaca tctgctctca ttacctatat
     1561 cgctttaact gccatatctc ttgtttgcgg tatacttagt ctggttctag catgctacct
     1621 aatgtacaag caaaaggcgc aacaaaagac cttgttatgg cttgggaata ataccctggg
     1681 tcagatgaga gccactacaa aaatgtgaat gcagatgaga ggcggaggta tccccaatag
     1741 caatttgtgt gcaaattct
//