From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Gossypium hirsutum vacuolar H+-ATPase catalytic subunit mRNA,
Date: 29 Dec 92 19:57:07 GMT
Distribution: bionet
Checksum: 58989 6

LOCUS       COTATPASE    2244 bp ss-mRNA            PLN       10-DEC-1992
DEFINITION  Gossypium hirsutum vacuolar H+-ATPase catalytic subunit mRNA,
            complete cds.
ACCESSION   L03186
KEYWORDS    H+-ATPase catalytic subunit.
SOURCE      Gossypium hirsutum (cultivar Acala SJ-2) anthesis ovule cDNA to
            mRNA.
  ORGANISM  Gossypium hirsutum
            Eukaryota; Plantae; Embryobionta; Magnoliophyta; Magnoliopsida;
            Dilleniidae; Malvales; Malvaceae.
REFERENCE   1  (bases 1 to 2244)
  AUTHORS   Wilkins,T.A.
  TITLE     Vacuolar H+-ATPAse 69 kD catalytic subunit cDNA from developing
            cotton ovules
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     mRNA            1..2244
                     /citation=[1]
                     /note="putative"
     misc_difference 558..854
                     /standard_name="Nonhomologous region unique to VATPases"
                     /citation=[1]
     misc_binding    924..947
                     /bound_moiety="ATP"
                     /citation=[1]
                     /note="putative"
     misc_feature    1512..1544
                     /citation=[1]
                     /note="ATP synthase alpha and beta subunit signatures;
                     putative"
     CDS             171..2042
                     /gene="cva69.24"
                     /evidence="EXPERIMENTAL"
                     /citation=[1]
                     /note="69 kilodalton"
                     /product="vacuolar H+-ATPase catalytic subunit"
                     /codon_start=1
                     /translation="MPAVYGSRLTTFEDSEKESEYGYVRKVSGPVVVADGMAGAAMYE
                     LVRVGHDNLIGEIIRLEGDSATIQVYEETAGLMVNDPVLRTHKPLSVELGPGILGNIF
                     DGIQRPLKTIAKRSGDVYIPRGVSVPALDKDALWDFQPKKIGEGDLLTGGDLYATVFE
                     NSLMQHHVALPPDAMGKITYIAPPGQYSLKDTVLELEFQGVKKQFTMLQTWPVRTPRP
                     VATKLAADTPLLTGQRVLDALFPSVLGGTCAIPGAFGCGKTVISQALSKYSNSDAVVY
                     VGCGERGNEMAEVLMDFPQLTMTLPDGREESVMKRTTLVANTSNMPVAAREASIYTGI
                     TIAEYFRDMGYNVSMMADSTSRWAEALREISGRLAEMPADSGYPAYLAARLASFYERA
                     GKVKCLGGPERTGSVTIVGAVSPPGGDFSDPVTSATLSIVQVFWGLDKKLAQRKHFPS
                     VNWLISYSKYSGALESFYEKFDPDFISIRTKAREVLQREDDLNEIVQLVGKDALAETD
                     KITLETAKLLREDYLAQNAFTPYDKFCPFYKSVWMMRNIVHFNALANQAVEKAAGMDG
                     QKITYSLIKHRLGDLFYRLVSQKFEDPAEGEEALVAKFKKLNEDLTAGFRALEDETR"
BASE COUNT      582 a    434 c    526 g    702 t
ORIGIN      
        1 ctcgattcaa acactagggt ttgatcgata attttattta taatttcctt tacttcaatt
       61 gcaattattc agatattcaa aatccaagct attttctttc atttaatctg aaattgattg
      121 gattttcctt ttaattttgt ttaatttaat ctaagaaaga agggggaaaa atgccggcag
      181 tttacggatc cagattgacg actttcgagg attcagaaaa agagagcgaa tacggttatg
      241 ttcgtaaggt gtctggacca gtcgtcgttg ctgatggaat ggctggtgct gccatgtatg
      301 aattagtccg tgttggacat gataatctaa ttggtgaaat tattcggttg gaaggagact
      361 ctgctacaat ccaagtttat gaagaaacag ctggcctgat ggttaatgat ccagttctgc
      421 ggacacacaa gcccctgtct gttgagttgg gaccaggaat attgggaaac atttttgatg
      481 gcattcagag gcctttgaaa actattgcca aaagatcggg tgatgtgtat atccctagag
      541 gagtttctgt cccagctctt gacaaagatg ctctttggga ctttcagcct aaaaaaatcg
      601 gtgagggaga ccttctaact ggtggagact tgtatgctac cgtctttgaa aacagtttga
      661 tgcaacatca tgttgccctg cctcctgatg caatgggaaa gataacttac attgctcctc
      721 ctggtcaata ttctttgaag gataccgtgc tggaacttga gtttcaaggt gtcaaaaagc
      781 aattcaccat gcttcagaca tggcctgtac gtacacctag acctgttgcg acaaaacttg
      841 ctgcagatac ccctctgctt actggccagc gtgttcttga tgctctattc ccctcggttc
      901 ttggtggaac ttgtgctatt cctggtgctt ttggctgtgg caaaactgtg attagtcaag
      961 ctctttctaa gtactcgaat tctgatgctg ttgtctatgt tggttgtggg gagcgtggaa
     1021 atgaaatggc tgaggttctt atggatttcc ctcaattgac aatgacattg cctgatggtc
     1081 gtgaagaatc tgtcatgaag cgcacaacac ttgtggctaa cacttctaac atgcctgtgg
     1141 cagctcgtga agcctcgatt tacacgggga tcactatagc tgaatacttc agagatatgg
     1201 gctacaatgt tagtatgatg gcagactcaa catctcgatg ggccgaagca ttgcgtgaaa
     1261 tttctggacg gctggcagaa atgcctgctg atagtggata tcctgcttat ctggctgcta
     1321 gattagcctc attttatgaa cgtgctggta aagttaaatg ccttggtggg ccagaacgta
     1381 caggtagtgt gacaattgtt ggtgctgttt ctcctcctgg aggagatttc tctgaccctg
     1441 tgacatctgc gaccctcagt attgtccagg ttttctgggg tcttgacaag aaacttgctc
     1501 agaggaagca tttcccttct gtcaactggc ttatttcata ctcaaagtac tcaggggcac
     1561 tggaatcttt ctatgagaaa tttgatcccg actttattag cattaggact aaggcccgag
     1621 aagtgcttca gagggaagat gatttgaatg aaattgtcca gcttgtagga aaggatgctc
     1681 tagctgaaac ggataagatc acactagaaa ctgctaaact tttaagggaa gattatcttg
     1741 ctcagaatgc ttttactcca tatgacaagt tctgtccgtt ctacaagtct gtttggatga
     1801 tgcgcaatat tgttcatttc aatgctttgg ccaaccaggc agtggagaaa gctgccggga
     1861 tggacggtca gaagataaca tacagtctta tcaagcatcg tctaggagat ctcttttatc
     1921 gtttagtgtc acaaaaattt gaagacccag cggaaggaga agaagccctg gtggcaaaat
     1981 tcaaaaagct taacgaggat cttacggctg gtttccgtgc attggaggat gaaactaggt
     2041 gaacaaaaag ttaaagatta gactttctcc ctacctatcc tagtccagat ttctctctct
     2101 tgtgtcttgc tatatgcatg tcgtgctggc gttgtagctt tttgttggca tgaactctag
     2161 agtggtatta gctgcgaatt tgttatttat tactttctgg cctttccctt tttgacagca
     2221 tgaaactgga tccttatttt tatt
//