From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Escherichia coli 4-hydroxybenzoate octaprenyl transferase (ubiA)
Date: 15 Aug 92 10:01:41 GMT
Distribution: bionet
Checksum: 38006     6

LOCUS       ECOUBIPLS    2000 bp ds-DNA             BCT       15-AUG-1992
DEFINITION  Escherichia coli 4-hydroxybenzoate octaprenyl transferase (ubiA)
            gene complete cds, chorismate lyase (ubiC) gene complete cds,
            sn-glycerol-3-phosphate acyltransferase (plsB) gene, 3' end.
ACCESSION   M93413
KEYWORDS    4-hydroxybenzoate octaprenyl transferase; chorismate lyase;
            plsB gene; sn-glycerol-3-phosphate acyltransferase; ubiA gene;
            ubiC gene.
SOURCE      Escherichia coli (sub_strain W3110, strain K-12) (library: Kohara
            lambda) DNA.
  ORGANISM  Escherichia coli
            Prokaryotae; Gram-negative facultatively anaerobic rods;
            Enterobacteriaceae.
REFERENCE   1  (bases 1 to 2000)
  AUTHORS   Lightner,V.A., Bell,R.M. and Modrich,P.
  TITLE     The DNA sequences encoding plsB and dgk loci of Escherichia coli
  JOURNAL   J. Biol. Chem. 258, 10856-10861 (1983)
  STANDARD  full staff_entry
REFERENCE   2  (bases 1 to 2000)
  AUTHORS   Nichols,B.P. and Green,J.M.
  TITLE     Cloning and sequencing of Escherichia coli ubiC and purification of
            Chorismate lyase
  JOURNAL   Unpublished (1992)
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     CDS             380..877
                     /function="enzymatic chorismate --> p-hydroxybenzoate +
                     pyruvate"
                     /note="N-terminal amino acid sequence of chorismate lyase
                     :NH2-SHPALTQLRALRYCEIPAL-"
                     /product="chorismate lyase"
                     /gene="ubiC"
                     /evidence=EXPERIMENTAL
                     /codon_start=1
                     /translation="MSHPALTQLRALRYCKEIPALDPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
                     /citation=[2]
     CDS             890..1762
                     /number=2
                     /function="enzymatic - 3-octaprenyl-4-hydroxybenzoate
                     synthesis"
                     /product="4-hydroxybenzoate octaprenyl transferase"
                     /gene="ubiA"
                     /evidence=EXPERIMENTAL
                     /codon_start=1
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSILVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
                     /citation=[2]
     CDS             complement(1917..2000)
                     /function="lysophosphatidic acid synthesis"
                     /EC_number="2.3.1.15"
                     /product="sn-glycerol-3-phosphate acyltransferase"
                     /gene="plsB"
                     /evidence=EXPERIMENTAL
                     /codon_start=1
                     /translation="MKVYQLLAELITSDVRLTIESATQGEG"
                     /citation=[1]
                     /partial
BASE COUNT      443 a    463 c    538 g    556 t
ORIGIN
        1 gaattccacg tatatctaca aatccagaca tgagatgttg cctgttaatc ttactcagga
       61 aacacttttc agctccaaat ctcatggtaa atatgcgctt ttccccattt ttactgcgag
      121 ttggcgagct catcgtataa tgaataaggg tgtttaagta aaggaaaaca tcaccgttcc
      181 tggcatcctg gacggtgatg cccctacggt tgccctcgcc agcacgggca tcggtaaagc
      241 gtaaggttca acatcgtttt accacttcat gcgattgttg cgtttttgtt gcgtattaga
      301 tcacttaatt tgctttacat ctcccgtaaa cacttttctg cgatacaatg cctttacgtt
      361 atgtaacgga gagttcggca tgtcacaccc cgcgttaacg caactgcgtg cgctgcgcta
      421 ttgtaaagag atccctgccc tggatccgca actgctcgac tggctgttgc tggaggattc
      481 catgacaaaa cgttttgaac agcagggaaa aacggtaagc gtgacgatga tccgcgaagg
      541 gtttgtcgag cagaatgaaa tccccgaaga actgccgctg ctgccgaaag agtctcgtta
      601 ctggttacgt gaaattttgt tatgtgccga tggtgaaccg tggcttgccg gtcgtaccgt
      661 cgttcctgtg tcaacgttaa gcgggccgga gctggcgtta caaaaattgg gtaaaacgcc
      721 gttaggacgc tatctgttca catcatcgac attaacccgg gactttattg agataggccg
      781 tgatgccggg ctgtgggggc gacgttcccg cctgcgatta agcggtaaac cgctgttgct
      841 aacagaactg tttttaccgg cgtcaccgtt gtactaagag gaaaaaaata tggagtggag
      901 tctgacgcag aataagctgc tggcgtttca tcgcttaatg cgtacggata agccaattgg
      961 cgcgttactg ctgctctggc caacattatg ggcgttgtgg gtggcgacac cgggcgttcc
     1021 ccagctctgg atcctggcgg tgtttgtcgc gggtgtctgg ctgatgcgcg ctgccggatg
     1081 tgtggtgaat gattatgctg accgcaagtt tgatggtcat gttaagcgca cggcgaaccg
     1141 accacttccc agcggcgcgg taacagagaa agaggcgcgc gcgctgtttg tcgtgctggt
     1201 actgatttcg tttttactgg tgctgacgct gaatacgatg accattctgt tgtcgattgc
     1261 cgcgctagcg ctggcgtggg tgtacccgtt tatgaagcgg tatacccatc taccgcaagt
     1321 ggtgctgggc gcggcgtttg gctggtcgat tccaatggct tttgccgctg tgagtgagtc
     1381 ggtgccattg agttgctggt taatgttcct cgccaatatt ctctgggcgg tggcttacga
     1441 cacgcagtat gcgatggttg accgcgatga tgatgtgaag attggcatta aatccacggc
     1501 aatcctgttc ggccaatacg ataaattgat tattggtatt ttgcagattg gcgtactggc
     1561 actgatggcg atcatcggtg agttaaatgg cttaggctgg ggatattact ggtcaattct
     1621 ggtggctggc gcgctgtttg tttatcaaca aaaactgatt gccaaccgcg agcgtgaagc
     1681 ctgctttaaa gcatttatga ataataacta tgttggtctg gtactatttt tagggctggc
     1741 aatgagttac tggcatttct gatgatgtaa aaaagccgga tgatcatccg gctttcttct
     1801 gggttgcctg atgcgcggcg cttctcaggc ctacacaaca catcgcaatt tattgaattt
     1861 gcagattatg gaaggccgga taaggcgttt tcgccgcatc cggcaattct ctctgattac
     1921 ccttcgccct gcgtcgcact ctcaatcgtc aaacgcacgt ctgatgtaat caactccgcc
     1981 agcaactgat aaaccttcat
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