From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: M-protein=165 kda structural constituent of myofibrillar M-band
Date: 29 Dec 92 21:28:16 GMT
Distribution: bionet
Checksum: 28052 10

LOCUS       S115719      5213 bp    mRNA            NLM       14-DEC-1992
DEFINITION  M-protein=165 kda structural constituent of myofibrillar M-band
            [chickens, pectoralis muscle, mRNA, 5213 nt]
ACCESSION   S46871 D11474
KEYWORDS    .
SOURCE      chickens pectoralis muscle
  ORGANISM  Unclassified.
            Unclassified.
REFERENCE   1  (bases 1 to 5213)
  AUTHORS   Noguchi,J., Yanagisawa,M., Imamura,M., Kasuya,Y., Sakurai,T.,
            Tanaka,T. and Masaki,T.
  TITLE     Complete primary structure and tissue expression of chicken
            pectoralis M-protein.
  JOURNAL   J. Biol. Chem. 267, 20302-20310 (1992)
  STANDARD  full automatic
COMMENT     This entry [NCBI gibbsq 115719] was created by the journal scanning
            component of NCBI/GenBank at the National Library of Medicine.
            This sequence comes from Fig. 3.  The authors begin their numbering
            at -83.  Zero is not present in the numbering.
FEATURES             Location/Qualifiers
     CDS             84..4436
                     /note="165 kda structural constituent of myofibrillar
                     M-band;  For the protein sequence (NCBI gibbsq 115720):
                     Method: conceptual translation with partial peptide
                     sequencing.  This sequence comes from Fig. 3."
                     /product="M-protein"
                     /codon_start=1
                     /translation="MSSVAVPFYQRRHKHFDQSYRNIQTRYVLEEYAARKAASRQAAH
                     YESTGLGKTTCRLCARRARSLAHEAMQESRKRTHEQKSHASDEKRIKFASELSSLERE
                     IHMARHHAREQLDRLAIQRMVEENMALERHVVEEKISRAPEILVRLRSHTVWEKMSVR
                     LCFTVQGFPSPVVQWYKNEELITPASDPAKYSVENKYGVHVLHINRADFDDSATYSAV
                     ATNIHGQASTNCAVVVRRFRESEEPHPAGIMPFHLPLSYDVCFTHFDVQFLEKFGVTF
                     ATEGETLTLKCSVLVTPELKRLRPRAEWYRDDVLIKDSKWTKLYFGEGQAALSFTHLN
                     KDDEGLYTLRMVTKGGVNECSAFLFVRDADALIAGAPGAPMDVKCHDANRDYVIVTWK
                     PPNTTSQNPVIGYFVDKCEVGLENWVQCNDAPVKICKYPVTGLYEGRSYIFRVRAVNS
                     AGISRPSRVSEPVAALDPVDLERTQTVHVDEGRKIVISKDDLEGDIQIPGPPTNVHAS
                     EISKTYVVLSWDPPVPRGREPLTYFIEKSMVGSGSWQRVNAQVAVKSPRYAVFDLAEG
                     KPYVFRVLSANKHGISDPSEITEPIQPQDIVVVPSAPGRVVATRNTKTSVVVQWDKPK
                     HEENLYGYYIDYSVVGSNQWEPANHKPINYNRFVVHGLETGEQYIFRVKAVNAVGFSE
                     NSQESEAIKVQAALTCPSYPHGITLLNCDGHSMTLGWKAPKYSGGSPILGYYIDKREA
                     NHKNWHEVNSSVISRTIYTVEDLTEDAFYEFKIAAANVVGIGHPSDPSEHFKCKAWTM
                     PEPGPAYDLTVCEVRNTSLVLLWKAPVYEGKSPITGYLVDYKEVDTEDWITANEKPTS
                     HRYFKVTDLHQGHTYVFKVRAVNDAGVGKSSEISEPVFVEASPGTKEIFSGVDEEGNI
                     YLGFECKEATDASHFLWGKSYEEIEDSDKFKIETKGDHSKLYFKHPDKSDLGTYCISV
                     SDTDGVSSSFVLDEEELERLMTLSNEIKNPTIPLKSELAYEVLDKGEVRFWIQAESLS
                     PNSTYRFVINDKEVENGDRHKISCDHSNGIIEMVMDKFTIDNEGTYTVQIQDGKAKNQ
                     SSLVLIGDAFKAILAESELQRKEFLRKQGPHFSEFLYWEVTEECEVLLACKIANTKKE
                     TVFKWYRNGSGIDVDEAPDLQKGECHLTVPKLSRKDEGVYKATLSDDRGHDVSTLELS
                     GKVYNDIILALSRVSGKTASPLKILCTEEGIRLQCFLKYYNEEMKVTWSHRESKISSG
                     EKMKIGGGEDVAWLQITEPTEKDKGNYTFEIFSDKESFKRTLDLSGQAFDDALTEFQR
                     LKAAAFAEKNRGKVIGGLPDVVTIMDGKTLNLTCTVFGNPDPEVVWFKNDKALELNEH
                     YLVSLEQGKYASLTIKGVTSEDSGKYSIYVKNKYGGETVDVTVSVYRHGEKIPEVNQG
                     QLAKPRLIPPSSST"
BASE COUNT     1587 a    992 c   1193 g   1441 t
ORIGIN      
        1 ctactaagct gggaaaggct ggtcttctct gctctgcagt atttctatat cccttggaat
       61 tgctgattgt aacaaactga agtatgtcat cagtagcagt gccattttac cagagaagac
      121 acaaacactt cgatcagtcc tatcgcaaca ttcagacaag atatgtactt gaagaatatg
      181 cagcaagaaa ggctgcttct agacaggctg ctcactatga atcaactggc ctgggaaaaa
      241 caacatgcag gctctgtgcc aggagggcac gcagcctggc tcatgaagca atgcaagaat
      301 ccaggaaaag gactcatgag caaaagtctc atgctagtga tgaaaaaagg atcaaatttg
      361 ccagtgagtt atcatctttg gaaagagaga ttcacatggc caggcaccat gctagggaac
      421 aactggatag acttgctata caaagaatgg tagaagaaaa tatggctctg gaaagacatg
      481 ttgttgagga aaaaataagt cgagctcctg aaatccttgt gcgcctgaga tctcacactg
      541 tctgggagaa gatgtctgtg cgtctttgct ttactgtaca aggatttccc tcccctgttg
      601 ttcagtggta caaaaatgag gaactaatta cccctgcaag tgatccagca aagtacagtg
      661 ttgaaaacaa atatggagta catgtgttgc atataaacag agctgatttt gatgattctg
      721 ctacctattc agctgttgca acaaatatcc atgggcaggc atcaactaat tgtgctgtgg
      781 ttgtgagaag gttcagagag agtgaagagc ctcacccagc tgggataatg cccttccact
      841 tgccactgtc gtatgatgtt tgctttacac actttgatgt ccaatttctg gagaaatttg
      901 gagtcacctt tgcaactgaa ggtgaaacac tgacccttaa atgttctgtg ttggtcaccc
      961 cagagctgaa aagactacgg cctcgtgctg agtggtatag agatgatgtt ctgataaagg
     1021 actctaagtg gacaaagctg tattttggag aaggccaggc agctctgtcc ttcactcatc
     1081 tgaacaaaga tgatgaaggt ttatacaccc tgcggatggt tacaaaaggt ggagtgaatg
     1141 aatgtagtgc attcctgttt gtcagagatg ctgacgcatt gattgcagga gcaccagggg
     1201 ctccaatgga tgttaaatgc catgatgcta acagagacta tgttattgtt acctggaaac
     1261 caccaaatac aacctctcag aacccagtca ttggatattt tgttgacaaa tgtgaagtag
     1321 gtttggagaa ctgggtccag tgcaatgatg cacctgtaaa aatctgcaag tatcccgtga
     1381 ctggtcttta tgaaggacgg tcttacatct tccgtgtgag agcagtgaac agtgctggaa
     1441 ttagccggcc ttctcgagtc tctgaaccag ttgcagctct tgaccccgtt gacctggaaa
     1501 gaacacaaac tgttcatgtg gatgaaggga gaaagattgt aatcagtaaa gatgacctcg
     1561 aaggcgatat tcagattcca ggccctccca ccaatgtaca tgcttcagaa atcagcaaaa
     1621 catatgttgt gctcagctgg gatccacctg ttcctcgtgg cagggagcca ctgacgtatt
     1681 tcattgagaa gtctatggtt ggcagtggaa gctggcaaag agtcaatgca caggttgctg
     1741 taaaatctcc tcgctatgca gtgtttgacc ttgcagaagg aaaaccatat gtcttccgag
     1801 tcttgtcagc aaacaagcac ggcatcagtg atccatctga aatcacagaa cctattcaac
     1861 cacaagatat tgttgttgtt ccatcagccc ctggtcgggt ggtggccaca aggaatacaa
     1921 aaacatcagt tgtagtccaa tgggacaaac caaaacatga agagaatttg tacggctact
     1981 acattgacta ttctgtggtg ggatctaatc aatgggaacc tgctaaccac aaacctatta
     2041 actataacag atttgttgtt catggtttgg aaactggtga acagtacatc ttccgtgtga
     2101 aagctgtgaa tgctgtagga ttcagtgaaa actcacagga gtcagaggct ataaaagtac
     2161 aagcagccct tacttgccca tcgtatcctc atggcatcac gcttctgaac tgtgatggtc
     2221 attcaatgac ccttggctgg aaagcaccga agtacagtgg aggttcccca atccttggtt
     2281 attatataga taaacgggaa gctaaccata aaaactggca tgaagtcaat tcttccgtta
     2341 ttagccgaac aatttatacg gtagaggatt tgacagaaga tgccttctat gagttcaaaa
     2401 ttgctgctgc caacgtggtt ggaataggtc atccttcaga tcccagtgag cactttaagt
     2461 gtaaagcctg gactatgcca gaacctggtc ctgcatatga tctcacagtt tgtgaagtta
     2521 gaaatacatc actggttctt ctgtggaaag ctcctgtgta tgaaggcaaa agccccatta
     2581 ctgggtactt agtggactat aaggaagtag atacagagga ctggatcact gctaatgaaa
     2641 aacctacttc acaccgttat tttaaggtta ccgatttaca tcagggtcat acctatgttt
     2701 tcaaagttcg tgcagtgaat gatgctgggg ttggcaagtc atctgagata tctgaaccag
     2761 tgtttgttga agcaagccct ggaacaaaag aaatcttttc aggtgtggat gaggagggca
     2821 atatttacct aggttttgaa tgcaaagaag ctacagatgc atctcatttt ctgtggggaa
     2881 aatcatatga ggagattgag gattctgata aatttaagat tgagacaaaa ggagatcatt
     2941 ctaagctgta cttcaagcat cctgataaat cggacttggg aacttactgt atttcagtta
     3001 gtgacacaga tggtgtttca tccagcttcg ttctggatga ggaagagctt gaacgtttga
     3061 tgacattaag caatgaaata aagaatccaa caatacctct gaaatcagaa ttagcttatg
     3121 aggtccttga taaaggagaa gtccgtttct ggattcaagc tgaaagctta tcaccaaatt
     3181 ctacctatag atttgttatt aatgataaag aagttgaaaa tggagatagg cacaagataa
     3241 gttgtgacca ttccaatggc attatagaaa tggtgatgga caaatttaca attgacaatg
     3301 aaggtaccta cacagtacag attcaagatg gaaaggccaa aaatcagtct tcactagttc
     3361 tcattggaga tgcatttaag gcgatattgg ctgagtctga gctccagcga aaggaattcc
     3421 tcaggaagca aggtccccac ttctcagagt ttctatattg ggaagttaca gaagaatgtg
     3481 aagttctgct tgcttgtaag attgcaaaca caaagaagga gactgttttc aagtggtata
     3541 ggaatggttc tgggattgac gttgatgagg cgcctgatct gcagaaggga gaatgtcacc
     3601 tcactgttcc caagctgtct cgcaaggatg aaggagtcta taaggcaaca ctgtcagatg
     3661 atagaggtca tgatgtctct actcttgaat tatcaggaaa agtgtataat gatataattt
     3721 tggcactgag cagagtcagt ggtaagacgg cttctccact gaagatactc tgcactgaag
     3781 aaggaataag gctccagtgt ttcttgaagt actacaatga agaaatgaaa gtcacctggt
     3841 ctcacaggga atctaagatt tcttctggtg aaaagatgaa gattggaggt ggagaggatg
     3901 ttgcttggct gcagataacc gaacccactg aaaaggataa ggggaattac acctttgaaa
     3961 tctttagtga caaggaatcc ttcaaacgca cacttgatct gtctgggcaa gcttttgatg
     4021 atgcccttac agaattccag cgactcaagg cagctgcttt tgctgagaag aatcgtggta
     4081 aagtcattgg tggtttgcct gatgttgtaa ctataatgga tgggaagact ctgaatctga
     4141 cctgtactgt gtttggaaac cctgacccag aagtggtttg gttcaagaat gacaaggccc
     4201 ttgagcttaa tgagcactat ttggtttcac tggaacaagg aaaatatgcc agtttaacaa
     4261 tcaaaggagt gacctcagaa gattcgggca aatatagcat ttatgtcaag aacaaatatg
     4321 gtggggaaac agtagatgtc actgtaagtg tatacagaca tggtgaaaaa ataccggaag
     4381 ttaaccaagg ccagcttgca aaacctagac taataccacc ttctagttct acctagaacc
     4441 caggggcaag ataaaatcca ataagtaaag cttgtaacta tgtttgatga acaaagtagt
     4501 atatgtgagc tgtagctgag gtattcccac agataactaa ttggtactct ccaaatggaa
     4561 tctgagcttt tagaagtttg ctttagggtg gtagctgtgc tacagcgctg tacacatgtg
     4621 cactgaaacc attatgacta catgtaatag ggtagtactt ctaaatagca aaaagccaaa
     4681 tgcctgcccg aagttcctat gggcaatggc agattgcata aacctacagt aaacctccat
     4741 ttctatacct ccaacaaaac ccattgagca ccctcttgag aaaattgaga acaaatgttt
     4801 catcaatgaa ggaagtgaac tgtagttact tgctcagaaa tgattcctgc tctgttacac
     4861 acacagtccc tttgctttga agcctctgat cgcataactt tagtggatag tctctattta
     4921 gtaattatat tgttatttta tagtgtgaat ttttctttag tagagctcag tagttttaca
     4981 gatgtttact gtttgctcat tgaagcatag gttacatggt tgtattttgc tttttttgga
     5041 ttgtgctaat aaaactttga aagtcacctt tgttactcac tgctccaaat cacacctaac
     5101 tagttttgtt tactctcttt cgttgtttaa ttattgctta ttgcacaatt tcacagctgg
     5161 tagcatgcag acaatcaact ttcaataaat gcaaattatg gttaatcaca gtc
//