From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Spinach chloroplast photosystem II gene encoding the 44kd and D2
Date: 25 Jul 92 10:06:39 GMT
Distribution: bionet
Checksum: 12905     9

LOCUS       SPICPPHTII   4381 bp ds-DNA             ORG       25-JUL-1992
DEFINITION  Spinach chloroplast photosystem II gene encoding the 44kd and D2
            chlorophyll alpha-binding proteins, complete cds.
ACCESSION   M27308 M12028 M16873
KEYWORDS    apoprotein; chlorophyll alpha-binding protein;
            photosystem II protein; reaction-center apoprotein;
            transfer RNA-Ser.
SOURCE      Spinach chloroplast DNA.
  ORGANISM  Chloroplast Spinacia oleracea
            Eukaryota; Plantae; Embryobionta; Magnoliophyta; Magnoliopsida;
            Caryophyllidae; Caryophyllales; Chenopodiaceae.
REFERENCE   1  (bases 1 to 4381)
  AUTHORS   Holschuh,K., Bottomley,W. and Whitfield,P.R.
  TITLE     Structure of the spinach chloroplast genes for the D2 and 44 kd
            reaction-centre proteins of photosystem II and for Ser-tRNA (UGA)
  JOURNAL   Nucleic Acids Res. 12, 8819-8834 (1984)
  STANDARD  simple staff_review
FEATURES             Location/Qualifiers
     CDS             1007..2068
                     /note="D2 photosystem II protein"
                     /codon_start=1
                     /translation="MTIAVGKFTKDEKDLFDSMDDWLWRDRFVFVGWSGLLLFPCAYF
                     ALGGWFTGTTFVTSWYTHGLASSYLEGCNFLTAAVSTPANSLAHSLLLLWGPEAQGDF
                     TRWCQLGGLWAFVALHGAFALIGFMLRQFELAWSVQLRPYNAIAFSGPIAVFVSVFLI
                     YPLGQSGWFFAPSFGVAAIFRFILFFQGFHNWTLNPFHMMGVAGVLGAALLCAIHGAT
                     VENTLFEDGDGANTFWAFNPTQAEETYSMVTANRFWSQIFGVAFSNKRWLHFFMLFVP
                     VTGLWMSALGVVGLALNLRAYDFVSQEIRAAEDPEFETFYTKNILLNEGIRAWMAAQD
                     QPHENLIFPEEVLPRGNAL"
     CDS             2016..3437
                     /note="44 kd photosystem II protein"
                     /codon_start=1
                     /translation="MKTLYSLRRFYPVETLFNGTLTLAGRDQETTGFAWWAGNARLIN
                     LSGKLLGAHVAHAGLIVFWAGAMNLFEVAHFVPEKPMYEQGLILLPHLATLGWGVGPG
                     GEVIDTFPYFVSGVLHLISSAVLGFGGIYHALLGPETLEESFPFFGYVWKDRNKMTTI
                     LGIHLILLGIGAFLLVFKALYFGGVYDTWAPGGGDVRKITNVTLSPSIIFGCLLKSPF
                     GGEGWIVSVDDLEDIIGGHVWIGVICILGGIWHILTKPFAWARRALVWSGEAYLSYSL
                     AALSVFGFIACCFVWFNNTAYPSEFYGPTGPEASQAQAFTFLVRDQRLGANVGSAQGP
                     TGLGKYLMRSPTGEVIFGGETMRFWDLRAPWLEPLRGPNGLDLSRLKKDIQPWQERRS
                     AEYMTHAPLGSLNSVGGVATEINAVNYVSPRSCLSTSHFVLGFFLFVGHLWHAGRARA
                     AAAGFEKGIDRDFEPVLSMTPLN"
     tRNA            complement(3688..3780)
                     /anticodon=(pos:3744..3746,aa:Ser)
                     /note="Ser-tRNA"
BASE COUNT     1196 a    781 c    904 g   1500 t
ORIGIN      HinfI site.
        1 gaatcagcaa ctatataata tttctattat ttttagaaaa aattgctgga atggaaagat
       61 tcgaaattca caaatgacaa agtaagtgga cttggcctat tgaatcatga atatacccgc
      121 tattctgata ttcaaatttg atagagatta aattggaaca gttgaccttt tttttcttta
      181 aactttgctt gaatttgtcg atatttccaa ttaaatcctt gtggtcctag ctattctata
      241 ggaataaaaa ttggcgagtc tcttcctcct agaggggaga aaacttttag ttcaaatcac
      301 aacctaaaaa agccattttc catatttttc ttttctttga ttccaaaacg gaatgaattt
      361 cgatcttatc tttcgaataa gattccaata tagtgtggct tcgtgtacca actatttcta
      421 tatccatgca tcccatattt ttgttccgac aatgagatgg agaatacatg cgtgaaagaa
      481 gctttttcct acgatatttt ttgaatatcg tatggaataa aaataaagta aaaaaataga
      541 aaattctccg tatatttctt tcttcgatcc gtgaaaatga aaagatatat tctgacattt
      601 ccgattgatc tgaaggaaaa aaactaactc taaacagaca aaaaataaac atatttatta
      661 gagaataatc tctatgtaac aataagctta atttgttgat agaaagaaaa ggttggtttg
      721 aagataaatc cgtaacgaga gaggagatcg gttgttcctt gatgagctct ttcaaaagat
      781 taatagttga gtctaataag ttaaaataaa ggaataattg aatttataaa tttactttaa
      841 cctaagttaa tttatgaacc actaaagaat ttagtttttc gaaaccctat agttaagggt
      901 cagtatacga gaaatcatcc ataaatgaaa tgatcgaatt ctcggactcc aaaaaatgct
      961 atggggtgct cggaaatggt tgaagtagtt gaataggagg atcactatga ctatagccgt
     1021 tggtaaattt accaaagacg aaaaagattt atttgatagt atggatgact ggttacggag
     1081 ggaccgtttt gtttttgtag gttggtctgg tctattgctc tttccttgtg cttatttcgc
     1141 cttagggggt tggtttacgg gtacaacctt tgtaacttca tggtataccc atggattggc
     1201 cagttcttat ttggaaggct gcaatttctt aaccgcagca gtttctactc ctgctaatag
     1261 tttagcccac tctttattgt tactgtgggg tcctgaagca caaggggatt ttactcgttg
     1321 gtgtcaatta ggtggcctgt gggcttttgt tgctcttcat ggtgctttcg cactaatagg
     1381 ttttatgtta cgtcaattcg aacttgctcg gtctgttcaa ttgcgccctt ataatgcaat
     1441 cgcattctct ggtccaattg ctgtttttgt ttctgtattc ctgatttatc cactaggtca
     1501 atctggttgg ttctttgcgc ctagttttgg tgtagcagct atattccgat tcattctctt
     1561 ttttcaaggg tttcataatt ggacgctgaa cccatttcat atgatgggag ttgccggtgt
     1621 attgggcgcc gctctgctat gcgctattca tggtgctacc gttgaaaata ctttatttga
     1681 agatggtgat ggggcaaata cattccgggc ttttaaccca acccaagctg aagaaactta
     1741 ttcaatggtc accgctaacc gcttttggtc ccaaatcttt ggggttgctt tttccaataa
     1801 acgttggtta catttcttta tgttatttgt accagtaacc ggtttatgga tgagtgctct
     1861 tggagtagtc ggtctggctt tgaacctacg tgcctatgac ttcgtttccc aggaaatccg
     1921 tgcagctgaa gatcctgaat ttgaaacttt ttacaccaaa aatattctct taaacgaggg
     1981 tatccgtgct tggatggcgg ctcaagatca gcctcatgaa aaccttatat tccctgagga
     2041 ggttctaccc cgtggaaacg ctctttaatg gaactttaac tttagccggc cgtgaccaag
     2101 aaaccaccgg tttcgcttgg tgggccggga atgctcgact tatcaattta tctggtaaac
     2161 tacttggagc tcatgtagcc catgccggat taatcgtatt ctgggccgga gcaatgaact
     2221 tatttgaagt ggctcatttc gtaccagaga agcctatgta tgaacaagga ttaattttac
     2281 ttccccacct agctactcta ggttgggggg taggccctgg cggggaagtt atagacactt
     2341 ttccatactt tgtatcggga gtacttcact taatttcgtc ggccgtattg ggctttggtg
     2401 gtatttatca tgcacttttg ggccctgaaa ctcttgaaga atcttttcca ttcttcggtt
     2461 atgtgtggaa agatagaaat aaaatgacca caattttggg cattcactta atcttgttag
     2521 gtataggcgc ttttcttcta gtattcaagg ctctttattt tgggggcgta tatgatacat
     2581 gggctcccgg aggtggagat gtaagaaaaa ttaccaacgt cacccttagt ccaagtatta
     2641 tatttggttg tttactaaaa tcgccttttg gaggagaagg gtggattgtt agtgtagacg
     2701 atttggaaga tataataggg gggcatgtat ggataggtgt catttgtata cttggtggaa
     2761 tctggcatat tttaacaaaa ccttttgcat gggctcgccg cgcacttgtc tggtctggcg
     2821 aggcttattt gtcttatagt ttggctgctt tatctgtctt tggtttcatt gcttgttgtt
     2881 ttgtctggtt caataatact gcttatccta gtgagtttta cgggcccact ggaccagaag
     2941 cttctcaagc tcaagcattt acttttttag ttagagacca acgtcttggg gctaacgtgg
     3001 gatccgctca aggacctact ggtttaggta agtatctaat gcgttctccg actggagaag
     3061 ttatttttgg gggcgaaact atgaggtttt gggatttgcg cgctccgtgg ttagaacccc
     3121 taagaggtcc aaacggtttg gacttgagtc gcctgaaaaa agatatacaa ccttggcaag
     3181 aacgacgttc agcagaatat atgactcatg ctcctttagg ttctttaaat tctgtgggtg
     3241 gggtggctac cgagatcaat gcagtcaatt atgtctctcc tagaagttgt ttatctactt
     3301 ctcattttgt tctaggattc ttcctattcg tagggcattt gtggcatgcg ggaagggctc
     3361 gtgcagctgc agcaggattt gaaaaaggaa ttgatcgtga ttttgaacct gttctttcga
     3421 tgacccctct taattgagac aagaaattca atgttttaag acaggaatgg aatcacttgg
     3481 attccatcat acatattaca tgttgggatc gggtcatacg taaaaagtct tccttttttt
     3541 ttcaactcat ttctatataa tttaatttct tttttttctg gctcggctag gtgagatagc
     3601 cgagccattc tctttaattt tatattttac cagacgaagc aacactaata aattcaaata
     3661 aggaaacagt tctattgaac gagcaaaagg agagagaggg attcgaaccc tcgatagttc
     3721 tgaataaaga actatgccgg ttttcaagac cggagctatc aaccactcag ccatctctcc
     3781 accataagtc aatttctatt ttattcttat tcccccgaac agaacatggt tatataagct
     3841 gataccatca caagttgtag aaaaatatta ggtgtaagtt gatagggtga tccatttatc
     3901 tgtatattat atataaaagt atgatctagc acacccattt aagtatttat taatattaat
     3961 taataaattc cccgtggttc tatctttgaa taaagtggta ataagtcata ttatatagaa
     4021 tcaatgaatt ccatagtaat agtggaaaat ccctacgtga tacatttttt acttttagta
     4081 aaatgactga tagagggatc aaatggtata gtttgttttt tgatagcttg gaggattata
     4141 cgtatgacta tagctttcca attggctgtt tttgcattaa ttgccacttc atcaatctta
     4201 ctgattagcg tacccgttgt atttgcttct cccgacggtt ggtcaagtaa caaaaatatt
     4261 gtattttccg gtacatcatt atggcttgga ttagtctttc tggtgggtat ccttaattct
     4321 ctcatctctt gaacctattt gttctagatc ctaaaatgac ccctcccccg aattcgaatt
     4381 c
//