From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Erwinia chrysanthemi pectate lyase a (pelA) gene, complete cds.
Date: 28 Jul 92 10:02:48 GMT
Distribution: bionet
Checksum: 36082     5

LOCUS       ERWPELAA     1647 bp ds-DNA             BCT       28-JUL-1992
DEFINITION  Erwinia chrysanthemi pectate lyase a (pelA) gene, complete cds.
ACCESSION   M77808
KEYWORDS    pectate lyase a.
SOURCE      Erwinia chrysanthemi (strain 3937) DNA.
  ORGANISM  Erwinia chrysanthemi
            Prokaryotae; Gram-negative facultatively anaerobic rods;
            Enterobacteriaceae.
REFERENCE   1  (sites)
  AUTHORS   Tamaki,S.J., Gold,S., Robeson,M., Manulis,S. and Keen,N.T.
  TITLE     Structure and organization of the pel genes from Erwinia
            chrysanthemi EC16
  JOURNAL   J. Bacteriol. 170, 3468-3478 (1988)
  STANDARD  full staff_review
REFERENCE   2  (bases 1 to 1647)
  AUTHORS   Favey,S., Bourson,C., Bertheau,Y., Kotoujansky,A. and Boccara,M.
  TITLE     Purification of the acidic pectate lyase and nucleotide sequence of
            the corresponding gene (pelA) of Erwinia chrysanthemi strain 3937
  JOURNAL   J. Gen. Microbiol. 138, 499-508 (1992)
  STANDARD  full staff_entry
FEATURES             Location/Qualifiers
     protein_bind    3..29
                     /bound_moiety="KdgR"
                     /gene="pelA"
     protein_bind    190..216
                     /bound_moiety="KdgR"
                     /gene="pelA"
     CDS             244..1422
                     /EC_number="4.2.2.2"
                     /product="pectate lyase a"
                     /gene="pelA"
                     /codon_start=1
                     /translation="MNKVSGRSFTRTSTCLLATLIAGVMTSGVSAAELVNSKALESAP
                     AAGWASQNGSTTGGAAATSDNIYVVTNISEFTSALSAGAVAKIIQITGTVDISGGTPY
                     KDFADQKARSQINIPANTTVIGIGTDAKFINGSLIIDGTDGTNNVIIRNVYIQTPIDV
                     EPHYEKGDGWNAEWDGMNITNGAHHVWVDHVTISDGSFTDDMYTTKDGETYVQHDGAL
                     DIKRGSDYVTISNSLFDQHDKTMLIGHSDTNSAQDKGKLHVTLFNNVFNRVTERAPRV
                     RYGSIHSFNNVFNGDVKDPVYRYLYSFGIGTSGSVLSEGNSFTIANLSASKACKVVKK
                     FNGSIFSDNGSVLNGSAADLSGCGFSAYTSAIPYVYAVQPMTTELAQSITDHAGSGKL
                     "
     sig_peptide     244..336
                     /gene="pelA"
                     /codon_start=1
     terminator      1443..1466
                     /gene="pelA"
BASE COUNT      461 a    464 c    354 g    368 t
ORIGIN      map position = 10 minutes
        1 gttacattta aaacatcgtt tcattatcat caaatattga aaaacaaata aatagatcgt
       61 attcattggc ggcccttaag tggcggcatc gccgaaactt caatattaat aataaaacat
      121 tattgatagg tgatgaggcg tatggcaggc agccatgaga ggacgataaa taaaaaataa
      181 cccatcgata tatatggaaa ttatttttaa caatcaacaa aggattttag taaggaaaat
      241 acgatgaaca aagtatcagg acgttctttt acccgcacct caacctgtct gctggctaca
      301 ttgatcgctg gcgtgatgac ctctggcgtt tccgctgccg aactggttaa cagcaaagcg
      361 ctggaatctg cgcccgccgc cggctgggcg tcacaaaacg gctccaccac cggcggtgcc
      421 gctgcaacca gcgataatat ctacgtcgtt accaatatca gtgaattcac cagcgcgctc
      481 agcgccggcg ccgtcgcaaa aatcatccag atcacaggca cggtcgatat cagcggcggc
      541 acgccctaca aagatttcgc tgatcagaaa gcccgcagcc agatcaacat tccggctaac
      601 accaccgtga tcgggattgg caccgacgcc aaattcatca acggctctct gattatcgac
      661 ggtaccgacg gcaccaataa cgtcatcatc cgcaacgtct acatccaaac gccaattgac
      721 gtagaaccgc attacgaaaa aggcgacggc tggaacgccg aatgggacgg catgaacatc
      781 accaacggtg cccatcatgt atgggtcgac cacgtcacca tcagcgacgg cagcttcacc
      841 gatgacatgt acaccaccaa agacggcgaa acttatgtcc agcacgatgg cgcactggat
      901 atcaaacgcg gttccgacta cgtgaccatc tccaacagcc tgttcgatca gcacgacaaa
      961 accatgctga ttggtcacag cgacaccaac tccgctcagg ataaaggtaa gctgcacgtc
     1021 acgctgttca acaacgtgtt taaccgcgta accgagcgcg cgccgcgcgt acgctacggc
     1081 agcatccaca gcttcaacaa cgtcttcaac ggtgatgtaa aagatccggt ataccgttac
     1141 ctgtacagct tcggtatcgg caccagcggc agcgtactgt ctgaaggcaa cagcttcacc
     1201 atcgccaatt tgagcgccag caaagcgtgc aaggttgtga agaaattcaa cggctccatt
     1261 ttctccgaca atggttccgt cctgaatggc agcgccgccg atctgtccgg ttgtggtttc
     1321 agcgcttaca ccagcgcgat tccttacgtt tacgccgttc agccgatgac taccgaactg
     1381 gcgcaatcca ttacggacca cgcaggttca ggcaagctgt aatttcccgt tcttttgttc
     1441 ccaacggctg cccacgggcg gccgttttta cgtctagccc acaccgtcat ccgcccgact
     1501 cgtccccttt cttcgtgcca atttcgtact cattcttatt aatttattac taattttcgc
     1561 attaacaccg ccagtcaaaa acagcagaat taattaacta tcaccgttcc aatcatcgaa
     1621 attaaaaata taaataattg atttaaa
//