From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Plasmid pAD1 (from Enterococcus faecalis) replication-associate
Date: 15 Sep 92 10:01:54 GMT
Distribution: bionet
Checksum: 54119    11

LOCUS       AD1REPABC    5145 bp ds-DNA             BCT       15-SEP-1992
DEFINITION  Plasmid pAD1 (from Enterococcus faecalis) replication-associate
            protein (repA, repB, and repC) genes, complete cds.
ACCESSION   L01794
KEYWORDS    repA gene; repB gene; repC gene; replication protein;
            replication-associated protein.
SOURCE      Plasmid pAD1 DNA.
  ORGANISM  Plasmid pAD1
            Prokaryotae.
REFERENCE   1  (bases 1 to 5145)
  AUTHORS   Weaver,K.E., Clewell,D.B. and An,F.Y.
  TITLE     DNA sequence and functional characterization of a region of E.
            faecalis plasmid pAD1 capable of supporting replication
  JOURNAL   Unpublished (1993)
  STANDARD  full staff_entry
REFERENCE   2  (bases 1 to 5145)
  AUTHORS   Weaver,K.E., Schmaderer,J.S. and Wood,D.D.
  TITLE     DNA sequence and characterization of a stability determinant for
            the E. faecalis plasmid pAD1
  JOURNAL   Unpublished (1993)
  STANDARD  full staff_entry
FEATURES             Location/Qualifiers
     CDS             complement(155..1165)
                     /function="essentail for replication"
                     /product="replication-associated protein"
                     /gene="repA"
                     /codon_start=1
                     /translation="MNNFKFFNVNRIYNELFYQFPKVLIASDKYKKMSDSTKIAYMLL
                     KARLEFAVQRNQVDENGNVYFTFTISELGEVLDCGKQKVLAIKSQLEEYGLLYQKQMG
                     FNKTLGKNNPNRLYLAELEVTENDIYQLQTFDDTMQENVDKSERMKIIPTPQRKTNAE
                     SLGNQEGMKIIPCQNVDKSEGMKISTVFNNLNTDTIDTIDTEKERLQQQLLLDQFSEV
                     QENTFLNKDSLKFIAAFSNTIQEAHEQVGTIIRAKTKVEKEYNLVLIGEDYQEEIDKC
                     LRRVMHKIKTDSTVKSPKGLFYKSFYNLFVECALEKKSQLNKKSNSNTPGVITHNWVE
                     NQ"
     RBS             complement(1170..1174)
                     /gene="repA"
     -10_signal      complement(1212..1217)
                     /gene="repA"
     -35_signal      complement(1236..1241)
                     /gene="repA"
     repeat_region   1277..1380
                     /function="'possible involvement in replication'"
     repeat_region   1457..1551
                     /function="'possible involvement in replication and
                     transcriptional controlofrepB'"
     -35_signal      1537..1542
                     /gene="repB"
     -10_signal      1561..1566
                     /gene="repB"
     repeat_unit     1570..1576
     RBS             1595..1599
                     /gene="repB"
     CDS             1606..2451
                     /function="involved in copy control"
                     /product="replication-associated protein"
                     /gene="repB"
                     /codon_start=1
                     /translation="MVKKIVFGNFKGGVGKTTNSVMVAYELAKKGFRVLVCDLDPQAN
                     STQLLRRTYGLQNNKELPIKETMMVAIQEGNLGKAVVNVMPNLYLLPSHKDFVNYPDF
                     LELTIMPTEKNYKERRIAFFSELLKPIENDYDYIIFDVPPTLSVFTDTALYSSNYIVI
                     VLQTQQRSLDGAEAFWEYLQTLYDTYKNIDFDIAGVLPVLLKNDSGIDNQIIKDAKDA
                     FGDETLFNTIVRHMERLKRYDRKGISEEGYTELYDFHDRKVHELYNKLSDEIIERTEG
                     TTANE"
     -35_signal      2389..2394
                     /gene="repC"
     -10_signal      2414..2419
                     /gene="repC"
     RBS             2432..2436
                     /gene="repC"
     CDS             2444..2815
                     /function="possibly involved in stable maintenance"
                     /product="replication-associated protein"
                     /gene="repC"
                     /codon_start=1
                     /translation="MSKYTFHKDPERKQSKEIKATEISEPETTFDLSSFSKEESSASD
                     SGSKRIGRPAKNKVYSTIRIQKSNINRINAFQNTLDFETQDDIVSFMLDRLDNTLEPE
                     QRTMFEMYMKAYESRDKKKQK"
     stem_loop       2831..2883
                     /function="'possible terminator'"
     repeat_region   2887..2910
                     /function="'required for replication'"
     misc_feature    4076..4360
                     /function="'involved in stable maintenance'"
BASE COUNT     1771 a    801 c    796 g   1777 t
ORIGIN
        1 tttaacaagt ttaacactat tctctgaaat gtgacttgtc ccaacaagat aatacgtttt
       61 attagacaca attatttttt ctacgttttt attttcagat tcaaaagaca tataattttc
      121 ctccataaat ttaaattcat actaaacagc tactttattg attttcaacc cagttatgag
      181 tgattactcc tggtgtgtta gaattacttt tcttgttcaa ttgacttttt ttctctagtg
      241 cacattcaac aaataaattg taaaaagact tgtaaaataa gcctttagga cttttaacag
      301 tagaatccgt cttgatttta tgcatcactc gtcgcaaaca tttgtctatt tcttcttgat
      361 aatcttcacc aattagaacg agattgtatt ctttttctac tttagtttta gcacgaataa
      421 ttgttcctac ttgctcatga gcttcttgaa tagtattaga gaaggcagca ataaatttta
      481 agctatcttt gtttaggaaa gtgttctctt gaacttctga aaactgatcg agcaataatt
      541 gttgttgcaa tcgttctttt tcagtatcta ttgtatctat tgtatctgta tttagattat
      601 taaatactgt actgattttc ataccctctg atttatcaac gttttggcag ggtatgattt
      661 tcataccctc ttgattacca agggattcag cgtttgtttt tctttggggg gtgggtatga
      721 ttttcatacg ctctgattta tcaacgtttt cttgcatagt atcatcgaat gtttgcaatt
      781 gataaatatc gttttctgtt acttcaagtt ccgctagata aagtctatta ggattgttct
      841 tacctagtgt tttattaaaa cccatttgtt tttgataaag caatccgtat tcttctaatt
      901 gtgattttat agctaatact ttctgtttac cgcaatcaag tacttctcct aattcactaa
      961 tagtaaatgt aaaatagaca tttccatttt catctacttg atttctttga acagcaaatt
     1021 ccagacgtgc ttttaaaagc atgtatgcaa tttttgtaga atctgacatt tttttgtatt
     1081 tatcgctagc aataagtact tttgggaatt gatagaatag ttcattgtaa atacggttta
     1141 cattaaaaaa tttgaagttg ttcatataag atccctcttt tcttagttaa aaatttatct
     1201 attaatttat ttagtaagcg ttcttttttt aagaatacca aaacattatt taaaataaag
     1261 caatttttat ttttagtagt aagatagtaa agtagtaaga tagtaaagta gtaagatagt
     1321 aaagtagtaa aatagtagaa tagtaaagta gtagaatagt aaaatagtaa gatagtaaaa
     1381 aatgtttatt ttttaataaa tacgtctaaa aatccttttg tagaaaggtt aatatcattc
     1441 ttttaaaaca ataagatagt aaaatagtaa gatagtaaag tagtaagata gtaaaatagt
     1501 aaagtagtag aatagtaaag tagtaggata gtaagatagt aaaatagtaa attttataaa
     1561 tattatttgt agtaagaaaa tatttatgaa atgaggagtc attgtatggt taaaaaaatt
     1621 gtatttggaa atttcaaagg cggcgttggg aagactacta attctgtaat ggtagcctat
     1681 gaattagcta aaaaaggttt tagagtttta gtttgtgact tagacccaca agcaaattca
     1741 acgcaattgt tgcgaagaac atatggatta caaaataata aagaactacc tataaaagaa
     1801 acgatgatgg tagcaattca agaaggaaat ttaggaaaag ctgttgtaaa tgtaatgcct
     1861 aatttgtatt tattgccttc acacaaagac tttgttaatt atcctgattt tcttgagcta
     1921 acaattatgc cgacagagaa aaactacaaa gagagacgaa tagcattttt tagtgaatta
     1981 cttaaaccaa ttgaaaatga ttatgattac attatatttg atgttcctcc tacactttca
     2041 gtatttaccg atacagctct ttattcttct aattacattg taattgtttt gcaaacgcag
     2101 caacgctccc ttgatggtgc agaagcattt tgggaatact tgcaaacctt gtatgatact
     2161 tataaaaaca ttgactttga tatcgctggg gtacttccag tgctgttgaa aaacgattct
     2221 ggaattgata atcaaataat taaagatgcc aaagacgctt ttggtgatga gactttgttt
     2281 aatacaattg ttagacacat ggaacggtta aaacgttatg atcgtaaagg tatatcagaa
     2341 gaaggatata cggaattata tgattttcat gatagaaaag ttcatgaatt gtataacaaa
     2401 ttatctgatg aaataattga acgaacagaa gggacgactg ctaatgagta agtatacatt
     2461 tcataaggat cctgaacgta aacaatcaaa agaaattaaa gcaactgaaa tttcagaacc
     2521 tgaaactact tttgatttaa gtagtttttc aaaagaagaa agttcagcaa gtgattcagg
     2581 atcaaaacga attgggcgac ctgctaaaaa taaagtatat agtacaatta ggatacaaaa
     2641 gagtaacatc aatagaataa atgcgtttca gaatacttta gattttgaaa cacaagacga
     2701 cattgtatcg ttcatgttag atagattaga taacacatta gaaccagaac aacgtacaat
     2761 gtttgaaatg tatatgaaag cttatgaatc acgagacaaa aagaaacaaa aataaaagat
     2821 actgtttaat aaaataaaaa cacaagaaat gctgtacaaa cattgctctt gtgtttttat
     2881 tttttgtagt aaaatagtaa gatagtaaaa aagtattttc attcaataat ttttttagtt
     2941 cgtctgtggc gttctaagtg tagcaaataa tcgtcttgca gttttaaaag ctctgaatag
     3001 acttggtcca tagtcttgct agcagataag gaaagagagt agataaagga gactggtaaa
     3061 gtttccattt ttttgttgcg agtgatccaa gaagaaatag ttccttgaga gaaggagtgt
     3121 agttcgcaaa attcctctac agtaatccct agttgcttaa tgatataagt attgattggg
     3181 tgtggatata caaaggtttt tctaggcatg gtgggtaagt ttcctttctt aatatattaa
     3241 tgcgtttgca gtaatatatt atcaaaaaac aagcaataaa ggaaatgctt gtgcccctta
     3301 ctacttgttt ttttatgaaa tagccttaaa agctctgtat aattaattat aaagtattgt
     3361 ccgtataatt agcactttat tattccatca gttttgaatt attttcaaag aaatcaatat
     3421 catcttttat tttaactaat atttcctcta gcattgtgaa acgtgtaaaa tattctttct
     3481 ttaactcaat accaaaattt gctgaaatat tcattacttt ttctatttct tcatctgaag
     3541 tcttattaaa gaaatacggt ggtgcatgtc tacaaaaatc attatacatt tctactgctg
     3601 atctaatagc atgtaaatag ggaggaaaaa tattatcaac tcctccaaag tgatacggct
     3661 cactttgact agataaaatt ttccgtatac tatcaacagc gacatcccca taggttactt
     3721 gcgcccatgc actactaggc aaccttcctg catcataaaa ccattgtgaa gctagttgaa
     3781 ttagctccat ttctttcaaa gaatacattg aattaccaaa aacattgtac tttaaatcca
     3841 taaaatcact ccttcttatt caattcaaaa gctcaaattt tcctaaaaaa ataccaatta
     3901 ttataactaa ctatacagat tcataactat aagtttatca ataaatatat ttttctacta
     3961 ctattttatt aataaaatag catcgatgaa aattgctacc actgtattct ttaccaattc
     4021 ctgcatagtc acaaaacttg acccaaaata aaaatatgct atactaaaag tgcgaaacga
     4081 cattaaatcg tacagataac acaaaaagca atcctacggc gaataggatt gctttttttt
     4141 acactagttg cgacttctag cgtgtgtgtt gatagcagct tactttcggc tatcgtcttc
     4201 ctcgtccaac acacgagaaa tcatttccag aaccaatcct acaaagattg gtgcgataac
     4261 caacgacatt aaatctttca caaataacac acctccaatc gaggcgagct gccgcattaa
     4321 ttatatcata atttgaaatt ctgccaattt aataataaaa ccttcaacaa tttatctagc
     4381 tgttttatga tcttttctaa gcgtgtaaac tcgtttacac caggaacatt tatacttgct
     4441 tgagtcattc gtaactctgc ttgctagaca gccacagctg cattctacat aagccacttg
     4501 gcgggcgcta tctcctagag gaataatggt accattataa tcatatgcct tcattacgaa
     4561 acccccaatc gctaaaatac tcatctaact ttttctgctc tttttttgaa aaaggagggg
     4621 agaccaaacc ttgaaaaggg tttgaattta ctctttcttc ttgttttatt ctgctagaat
     4681 gacgatacat acacacggag tcaatttctt ctatactata ggaatcttca ccaattatta
     4741 ttttgttttc ttccgcatat cctcgaaaag ttcctataac tggtatagga aaaggattgg
     4801 tttctgaaga cataagtaat aaatataatg gcttattttg tttgattgag cgagaaaaat
     4861 aagaagaaaa ttcttttcta ttttgcttca tttggttgta cctaagccaa cagttccacc
     4921 tgcatggcca ccaacaaggc ttgctcgtga aattgctgtg gcgccttcca tcagtgaaga
     4981 agcatgtatc agagctttaa acccaaattt ctgacgtatt ttatcaataa gaaaatacat
     5041 atccgtttca tgaatttgct cctccggaac agaaaataaa tccagctgta aatttttgtt
     5101 ccatacaaga cggccataag atacccctaa atttcgtacg tcgac
//