From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Zebrafish homeobox hox2.2 (hox[zf-22]) gene, complete cds and ORF
Date: 6 Nov 92 18:08:57 GMT
Distribution: bionet
Checksum: 16045 9

LOCUS       ZEFHOX22     3442 bp ds-DNA             VRT       28-OCT-1992
DEFINITION  Zebrafish homeobox hox2.2 (hox[zf-22]) gene, complete cds and ORF
            1-2.
ACCESSION   X17267
KEYWORDS    DNA-binding protein; homeo box; hox2.2; hox[zf-22] gene.
SOURCE      Brachydanio rerio DNA.
  ORGANISM  Brachydanio rerio
            Eukaryota; Animalia; Chordata; Vertebrata; Osteichthyes;
            Actinopterygii; Cypriniformes; Cyprinoidei; Cyprinidae.
REFERENCE   1  (bases 1 to 3442)
  AUTHORS   Njolstad,P.R.
  JOURNAL   Unpublished (1989)
  STANDARD  full automatic
REFERENCE   2  (bases 1 to 3442)
  AUTHORS   Njolstad,P.R., Molven,A., Apold,J. and Fjose,A.
  TITLE     The zebrafish homeobox gene hox-2.2: transcription unit, potential
            regulatory regions and in situ localization of transcripts
  JOURNAL   EMBO J. 9, 515-524 (1990)
  STANDARD  full automatic
COMMENT     SWISS-PROT; P15861; HM22$BRARE.
               *source: developmental stage=adult; tissue=embryo; *source:
            clone= lambda C21;
               From EMBL 27   entry BRHOX22;  dated 12-MAR-1991.
FEATURES             Location/Qualifiers
     mRNA            join(<1001..1430,2484..>3442)
                     /gene="hox2.2"
                     /partial
     exon            <1001..1430
                     /gene="hox2.2"
     misc_binding    25..33
                     /bound_moiety="unspecified"
                     /note="homeodomain binding site (u1)"
                     /note="putative"
     CAAT_signal     426..434
                     /note="putative"
     TATA_signal     475..481
                     /note="putative"
     precursor_RNA   510..3442
                     /note="primary transcript"
     misc_binding    513..522
                     /bound_moiety="unspecified"
                     /note="homeodomain binding site (u2)"
                     /note="putative"
     intron          1431..2483
                     /number=1
                     /gene="hox2.2"
     misc_binding    1696..1703
                     /bound_moiety="unspecified"
                     /note="homeodomain binding site (i1)"
                     /note="putative"
     misc_binding    1904..1913
                     /bound_moiety="unspecified"
                     /note="homeodomain binding site (i2)"
                     /note="putative"
     exon            2484..>3442
                     /number=2
                     /gene="hox2.2"
     polyA_signal    3023..3028
                     /gene="hox2.2"
     polyA_signal    3254..3259
                     /gene="hox2.2"
     misc_binding    3313..3322
                     /bound_moiety="unspecified"
                     /note="homeodomain binding site (d1)"
                     /note="putative"
     CDS             join(1001..1430,2484..2735)
                     /partial
                     /note="hox2.2"
                     /codon_start=1
                     /translation="MSSYFLNSTFPVTLPGGQESFLGQIPLYSSGYTDPLRHYPGAAY
                     GGSSVQEKAYPSSFYQQANGAYSRATAAGPCDYATASFYREKDPACALASIEEHSFVL
                     SQDHRKTDCTGSTGKSIYPEADEQKPSAPVYPWMQRMNSCNGTFGNAGRRGRQTYTRY
                     QTLELEKEFHFNRYLTRRRRIEIAHALCLTERQIKIWFQNRRMKWKKENKLINCSQTS
                     GEEEEEKRT"
     CDS             551..925
                     /note="ORF 1"
                     /codon_start=1
                     /translation="ENRRLRGLYVDTLLSNGMIGSKQTVCGGTKCRCIQFSVLKFLCD
                     SYCAISLEGTGTENGLKKASSLLWGPPARAITAPLEVLFCRHVRLLLVSGWSDGTVSL
                     CPALAHSHPEHPPTPQLKPNLR"
     CDS             974..1474
                     /note="ORF 2"
                     /codon_start=1
                     /translation="TKQKSRTLPMSSYFLNSTFPVTLPGGQESFLGQIPLYSSGYTDP
                     LRHYPGAAYGGSSVQEKAYPSSFYQQANGAYSRATAAGPCDYATASFYREKDPACALA
                     SIEEHSFVLSQDHRKTDCTGSTGKSIYPEADEQKPSAPVYPWMQRMNSCNGKLMLSFL
                     PFIFNT"
BASE COUNT     1071 a    681 c    660 g   1030 t
ORIGIN      
        1 gtcgacaaaa agctacaaca cagctgaatt atttgctggc tgtcttaaac cattaatttt
       61 tgtaaaggag gaaacatctt ggacgaaatt ctgtgtggag gttttagttt tatttgtata
      121 aaatttattt aacaacacta tacagaagtt ctttcttaac aaattgtggg agacatttgg
      181 attgttatgt agtcattcgc aactaaaaac ctgatattat aaatatttat aattaacgtt
      241 atcactcatt tttattgtat atttgtaata aacagctaaa catgtatttg cttttgttaa
      301 atagcttcgc tcaggctaat ttactttgca cagtttatat ataggcttgc ttttctttaa
      361 cacgtcgttt accatttata aatcactatt aaattacagt gttgaaaaag cgctgaagcc
      421 gcaatcatca atatgatcat catatctatt gtgacttact agtgtgataa ggcctataaa
      481 ttacctattt gaaatacaaa taatgaaaga tgaataatta tctaatactg tacggaataa
      541 tgtatgctag gaaaatcggc gactccgggg cttgtatgtt gacacgctac tctccaatgg
      601 tatgatagga tcaaagcaga ctgtatgcgg gggcacaaag tgtcgatgta ttcaatttag
      661 cgttttaaaa ttcctatgtg attcgtattg tgcaatctca ttggaaggaa cgggaaccga
      721 gaacggatta aaaaaagcta gctctctgct ctggggtccc ccggcgcgtg caatcacagc
      781 ccccctcgag gtgctatttt gcaggcacgt ccgcttgcta ttggtcagtg gctggtcaga
      841 tggtacggtt tccctctgtc ccgcactggc acatagccac ccagaacacc cccccacccc
      901 ccaactcaaa cccaatctcc gataaactac taatagctaa accacttgga ctataaaaca
      961 caacaaatca taaaccaagc aaaagagccg taccctccca atgagttcct attttctaaa
     1021 ctcaactttc ccagtgactc tgcccggagg acaggagtct ttcttgggac agataccgtt
     1081 atattcctcc ggatacacag accctttacg acactatccc ggtgctgctt atggaggttc
     1141 cagtgtccaa gagaaggcgt acccatcctc cttttaccaa caagcgaacg gcgcttacag
     1201 cagggcaacc gccgctggtc catgcgatta tgcgacagca agcttttaca gggaaaagga
     1261 ccctgcctgc gccctcgcca gcatagagga gcactcgttc gttctcagtc aggatcatcg
     1321 caagacagac tgcacgggct cgacggggaa aagcatctac cctgaggccg acgaacagaa
     1381 gccgtcagcg ccggtttacc cttggatgca acggatgaac tcatgtaacg gtaagttaat
     1441 gttatctttt ttgcctttca tttttaatac gtagctagtg cgtactatac gtagcctaac
     1501 ttaccgcttc actgtaaaat gccggaagcc catacagtgc aaatgcgcat tctctctacc
     1561 atagtcatac tgaaaggaaa gccactcctg taaagttctg tgaaatctca tatgtacagt
     1621 acttgttttt gagggaggac gtttagaaat atgtctgcgc aaggctcata tcatttaaat
     1681 caccgaataa gcttctctaa ttataaacag aagagctgct gcaccaccgc atgtcttact
     1741 acaaaaatga ccattgtttt tgttttgaca catctgttgc tgtttgtgtc ttcttgttat
     1801 aagaaaaata gacagtacgt ggtagtatag aaaatatgaa ttatattctg aaagactacg
     1861 caatagttta tgaaacagtg ccaacaacag aagctgatgt tttgtcgatt atctataaca
     1921 agatatcatt tgctagcctg ccggttgaca gtaacgtgtt ttataacaaa aataaactaa
     1981 aaaccagcag agtgaacaga catcttgtac acgtacacgt ttcagtcaca aacaaaactt
     2041 tgacaagtgc tcaaactagt aggcatggtg tctatgttca gattctaagt ttgatatgga
     2101 tcatataaat agacctgtat cttttgctta tatctgtcac gaaaataagt agaataacat
     2161 ttcctcagct tttccctcat ctcaaaataa tagtttaaga cttcccaatt taagtacatt
     2221 gaagtctaaa cctgtgtgac ctatttaatt taaaaattat tacaaatttt ctcaagaaac
     2281 acgaatgagt gtgttaaaag gtaatatagc aaaaatgtgc aatgttgaag ggtgtattta
     2341 aggaatgcat taacctatcg cactgaatag agtttcttgt cattatgtca ccctcgagct
     2401 gttttctacg gacgcattta tcattgtaat aaacacgctc tcggtgttaa tgtttgacta
     2461 ttctctttta tcctcatgga cagggacctt cggtaacgct ggtcgaaggg gtcgccagac
     2521 gtacactcgc taccagacgt tagaattgga gaaagagttc cactttaaca ggtatctgac
     2581 ccgaagacgc cgcattgaga ttgcgcacgc actgtgcctg acggaacgac agataaagat
     2641 atggttccaa aaccggcgaa tgaaatggaa aaaggagaac aaactgatca actgttcaca
     2701 aaccagtgga gaagaggagg aggagaagag gacagaatga aattttaaat cataaaatac
     2761 tccaaaagaa aactatggca tagagggcac cagactctct tataacacaa atgaagactt
     2821 tccatcatgt tagcaactgt agcattcctg gtaactcatt gtaatttctt ttgtgattct
     2881 ttttttttta catgtcgttt aacatgtggt tttgtgcgct cttactctga ctttagattt
     2941 gctcttatga ctgaaatgtc catgtggaac gaagctattt gaaatttgtg tcatattcgt
     3001 gtttatattt aagctgtgta agaataaaaa attcaattgt acggaaaatg ttttatcttc
     3061 tattttttgc aaactaaact ttcagacact tggacataat ttattgagtc tgggggaaaa
     3121 ggtgtggaag tagctagttt caaaaggaac gtaaaagaaa gtagtctgca acagtacaca
     3181 ctttacttat attaatgtgc actgtattac atttcatatt taatacgtgc ggattttttc
     3241 tattaaagac ttgaataaat ataaagcaac tatttcgcct tcatgacatg ttagctgatg
     3301 acggtgaaaa tgttaaatta ttggtcctca ttgcggtgta acgcctgtat actttcatac
     3361 atttttgtca agaaaactag cttaaacaaa ttaagtgatc taatgactga cccgtaaatt
     3421 atgcaaggag aaataagtcg ac
//