From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Human glycogen debranching enzyme mRNA, complete cds.
Date: 21 Jul 92 10:05:35 GMT
Distribution: bionet
Checksum: 19985    12

LOCUS       HUMMGDBEA    6961 bp ss-mRNA            PRI       21-JUL-1992
DEFINITION  Human glycogen debranching enzyme mRNA, complete cds.
ACCESSION   M85168
KEYWORDS    glycogen debranching enzyme.
SOURCE      Homo sapiens muscle cDNA to mRNA.
  ORGANISM  Homo sapiens
            Eukaryota; Animalia; Chordata; Vertebrata; Mammalia; Theria;
            Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE   1  (bases 1 to 6961)
  AUTHORS   Yang,B.-Z., Ding,J.-H., Enghild,J.J., Bao,Y. and Chen,Y.-T.
  TITLE     Molecuar cloning and nucleotide sequence of cDNA encoding human
            muscle glycogen debranching enzyme
  JOURNAL   J. Biol. Chem. 267, 9294-9299 (1992)
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     CDS             46..4593
                     /EC_number="2.4.1.25"
                     /EC_number="3.2.1.33"
                     /product="glycogen debranching enzyme"
                     /codon_start=1
                     /translation="MSLLTCAFYLGYELQFRLGPTLQGKAVTVYTNYPFPGETFNREK
                     FRSLDWENPTEREDDSDKYCKLNLQQSGSFQYYFLQGNEKSGGGYIVVDPILRVGADN
                     HVLPLDCVTLQTFLAKCLGPFDEWESRLRVAKESGYNMIHFTPLQTLGLSRSCYSLAN
                     QLELNPDFSRPNRKYTWNDVGQLVEKLKKEWNVICITDVVYNHTAANSKWIQEHPECA
                     YNLVNSPHLKPAWVLDRALWRFSCDVAEGKYKEKGIPALIENDHHMNSIRKIIWEDIF
                     PKLKLWEFFQVDVNKAVEQFRRLLTQENRRVTKSDPNQHLTIIQDPEYRRFGCTVDMN
                     IALTTFIPHDKGPAAIEECCNWFHKRMEELNSEKHRLINYHQEQAVNCLLGNVFYERL
                     AGHGPKLGPVTRKHPLVTRYFTFPFEEIDFSMEESMIHLPNKACFLMAHNGWVMGDDP
                     LRNFAEPGSEVYLRRELICWGDSVKLRYGNKPEDCPYLWAHMKKYTEITATYFQGVRL
                     DNCHSTPLHVAEYMLDAARNLQPNLYVVAELFTGSEDLDNVFVTRLGISSLIREAMSA
                     YNSHEEGRLVYRYGGEPVGSFVQPCLRPLMPAIAHALFMDITHDNECPIVHRSAYDAL
                     PSTTIVSMACCASGSTRGYDELVPHQISVVSEERFYTKWNPEALPSNTGEVNFQSGII
                     AARCAISKLHQELGAKGFIQVYVDQVDEDIVAVTRHSPSIHQSVVAVSRTAFRNPKTS
                     FYSKEVPQMCIPGKIEEVVLEARTIERNTKPYRKDENSINGTPDITVEIREHIQLNES
                     KIVKQAGVATKGPNEYIQEIEFENLSPGSVIIFRVSLDPHAQVAVGILRNHLTQFSPH
                     FKSGSLAVDNADPILKIPFASLASRLTLAELNQILYRCESEEKEDGGGCYDIPNWSAL
                     KYAGLQGLMSVLAEIRPKNDLGHPFCNNLRSGDWMIDYVSNRLISRSGTIAEVGKWLQ
                     AMFFYLKQIPRYLIPCYFDAILIGAYTTLLDTAWKQMSSFVQNGSTFVKHLSLGSVQL
                     CGVGKFPSLPILSPALMDVPYRLNEITKEKEQCCVSLAAGLPHFSSGIFRCWGRDTFI
                     ALRGILLITGRYVEARNIILAFAGTLRHGLIPNLLGEGIYARYNCRDAVWWWLQCIQD
                     YCKMVPNGLDILKCPVSRMYPTDDSAPLPAGTLDQPLFEVIQEAMQKHMQGIQFRERN
                     AGPQIDRNMKDEGFNITAGVDEETGFVYGGNRFNCGTWMDKMGESDRARNRGIPATPR
                     DGSAVEIVGLSKSAVRWLLELSKKNIFPYHEVTVKRHGKAIKVSYDEWNRKIQDNFEK
                     LFHVSEDPSDLNEKHPNLVHKRGIYKDSYGASSPWCDYQLRPNFTIAMVVAPELFTTE
                     KAGKALEIAEKKLLGPLGMKTLDPDDMVYCGIYDNALDNDNYNLAKGFNYHQGPEWLW
                     PIGYFLRAKLYFSRLMGPETTAKTIVLVKNVLSRHYVHLERSPWKGLPELTNENAQYC
                     PFSCETQAWSIATILETLYDL"
BASE COUNT     2252 a   1149 c   1364 g   2196 t
ORIGIN
        1 taaaataaaa catctgtttt tcaatgtggt aatttaagtc ctacgatgag tttattaaca
       61 tgtgcttttt atttagggta tgagctacag ttccgattag gcccaacttt acagggaaaa
      121 gcagttaccg tgtatacaaa ttacccattt cctggagaaa catttaatag agaaaaattc
      181 cgttctctgg attgggaaaa tccaacagaa agagaagatg attctgataa atactgtaaa
      241 cttaatctgc aacaatctgg ttcatttcag tattatttcc ttcaaggaaa tgagaaaagt
      301 ggtggaggtt acatagttgt ggaccccatt ttacgtgttg gtgctgataa tcatgtgcta
      361 cccttggact gtgttactct tcagacattt ttagctaagt gtttgggacc ttttgatgaa
      421 tgggaaagca gacttagggt tgcaaaagaa tcaggctaca acatgattca ttttacccca
      481 ttgcagactc ttggactatc taggtcatgc tactcccttg ccaatcagtt agaattaaat
      541 cctgactttt caagacctaa tagaaagtat acctggaatg atgttggaca gctagtggaa
      601 aaattaaaaa aggaatggaa tgttatttgt attactgatg ttgtctacaa tcatactgct
      661 gctaatagta aatggatcca ggaacatcca gaatgtgcct ataatcttgt gaattctcca
      721 cacttaaaac ctgcctgggt cttagacaga gcactttggc gtttctcctg tgatgttgca
      781 gaagggaaat acaaagaaaa gggaatacct gctttgattg aaaatgatca ccatatgaat
      841 tccatccgaa aaataatttg ggaggatatt tttccaaagc ttaaactctg ggaatttttc
      901 caagtagatg tcaacaaagc ggttgagcaa tttagaagac ttcttacaca agaaaatagg
      961 cgagtaacca agtctgatcc aaaccaacac cttacgatta ttcaagatcc tgaatacaga
     1021 cggtttggct gtactgtaga tatgaacatt gcactaacga ctttcatacc acatgacaag
     1081 gggccagcag caattgaaga atgctgtaat tggtttcata aaagaatgga ggaattaaat
     1141 tcagagaagc atcgactcat taactatcat caggaacagg cagttaattg ccttttggga
     1201 aatgtgtttt atgaacgact ggctggccat ggtccaaaac taggacctgt cactagaaag
     1261 catcctttag ttaccaggta ttttactttc ccatttgaag agatagactt ctccatggaa
     1321 gaatctatga ttcatctgcc aaataaagct tgttttctga tggcacacaa tggatgggta
     1381 atgggagatg atcctcttcg aaactttgct gaaccgggtt cagaagttta cctaaggaga
     1441 gaacttattt gctggggaga cagtgttaaa ttacgctatg ggaataaacc agaggactgt
     1501 ccttatctct gggcacacat gaaaaaatac actgaaataa ctgcaactta tttccaggga
     1561 gtacgtcttg ataactgcca ctcaacacct cttcacgtag ctgagtacat gttggatgct
     1621 gctaggaatt tgcaacccaa tttatatgta gtagctgaac tgttcacagg aagtgaagat
     1681 ctggacaatg tctttgttac tagactgggc attagttcct taataagaga ggcaatgagt
     1741 gcatataata gtcatgaaga gggcagatta gtttaccgat atggaggaga acctgttgga
     1801 tcctttgttc agccctgttt gaggccttta atgccagcta ttgcacatgc cctgtttatg
     1861 gatattacgc atgataatga gtgtcctatt gtgcatagat cagcgtatga tgctcttcca
     1921 agtactacaa ttgtttctat ggcatgttgt gctagtggaa gtacaagagg ctatgatgaa
     1981 ttagtgcctc atcagatttc agtggtttct gaagaacggt tttacactaa gtggaatcct
     2041 gaagcattgc cttcaaacac aggtgaagtt aatttccaaa gcggcattat tgcagccagg
     2101 tgtgctatca gtaaacttca tcaggagctt ggagccaagg gttttattca ggtgtatgtg
     2161 gatcaagttg atgaagacat agtggcagta acaagacact cacctagcat ccatcagtct
     2221 gttgtggctg tatctagaac tgctttcagg aatcccaaga cttcatttta cagcaaggaa
     2281 gtgcctcaaa tgtgcatccc tggcaaaatt gaagaagtag ttcttgaagc tagaactatt
     2341 gagagaaaca cgaaacctta taggaaggat gagaattcaa tcaatggaac accagatatc
     2401 acagtagaaa ttagagaaca tattcagctt aatgaaagta aaattgttaa acaagctgga
     2461 gttgccacaa aagggcccaa tgaatatatt caagaaatag aatttgaaaa cttgtctcca
     2521 ggaagtgtta ttatattcag agttagtctt gatccacatg cacaagtcgc tgttggaatt
     2581 cttcgaaatc atctgacaca attcagtcct cactttaaat ctggcagcct agctgttgac
     2641 aatgcagatc ctatattaaa aattcctttt gcttctcttg cctccagatt aactttggct
     2701 gagctaaatc agatccttta ccgatgtgaa tcagaagaaa aggaagatgg tggagggtgc
     2761 tatgacatac caaactggtc agcccttaaa tatgcaggtc ttcaaggttt aatgtctgta
     2821 ttggcagaaa taagaccaaa gaatgacttg gggcatcctt tttgtaataa tttgagatct
     2881 ggagattgga tgattgacta tgtcagtaac cggcttattt cacgatcagg aactattgct
     2941 gaagttggta aatggttgca ggctatgttc ttctacctga agcagatccc acgttacctt
     3001 atcccatgtt actttgatgc tatattaatt ggtgcatata ccactcttct ggatacagca
     3061 tggaagcaga tgtcaagctt tgttcagaat ggttcaacct ttgtgaaaca cctttcattg
     3121 ggttcagttc aactgtgtgg agtaggaaaa ttcccttccc tgccaattct ttcacctgcc
     3181 ctaatggatg taccttatag gttaaatgag atcacaaaag aaaaggagca atgttgtgtt
     3241 tctctagctg caggcttacc tcatttttct tctggtattt tccgctgctg gggaagggat
     3301 acttttattg cacttagagg tatactgctg attactggac gctatgtaga agccaggaat
     3361 attattttag catttgcggg taccctgagg catggtctca ttcctaatct actgggtgaa
     3421 gggatttatg ccagatacaa ttgtcgggat gctgtgtggt ggtggctgca gtgtatccag
     3481 gattactgta aaatggttcc aaatggtcta gacattctca agtgcccagt ttccagaatg
     3541 tatcctacag atgattctgc tcctttgcct gctggcacac tggatcagcc attgtttgaa
     3601 gtcatacagg aagcaatgca aaaacacatg cagggcatac agttccgaga aaggaatgct
     3661 ggtccccaga tagatcgaaa catgaaggac gaaggtttta atataactgc aggagttgat
     3721 gaagaaacag gatttgttta tggaggaaat cgtttcaatt gtggcacatg gatggataaa
     3781 atgggagaaa gtgacagagc tagaaacaga ggaatcccag ccacaccaag agatgggtct
     3841 gctgtggaaa ttgtgggcct gagtaaatct gctgttcgct ggttgctgga attatccaaa
     3901 aaaaatattt tcccttatca tgaagtcaca gtaaaaagac atggaaaggc tataaaggtc
     3961 tcatatgatg agtggaacag aaaaatacaa gacaactttg aaaagctatt tcatgtttcc
     4021 gaagaccctt cagatttaaa tgaaaagcat ccaaatctgg ttcacaaacg tggcatatac
     4081 aaagatagtt atggagcttc aagtccttgg tgtgactatc agctcaggcc taattttacc
     4141 atagcaatgg ttgtggcccc tgagctcttt actacagaaa aagcagggaa agctttggag
     4201 attgcagaaa aaaaattgct tggtcccctt ggcatgaaaa ctttagatcc agatgatatg
     4261 gtttactgtg gaatttatga caatgcatta gacaatgaca actacaatct tgctaaaggt
     4321 ttcaattatc accaaggacc tgagtggctg tggcctattg ggtattttct tcgtgcaaaa
     4381 ttatattttt ccagattgat gggcccggag actactgcaa agactatagt tttggttaaa
     4441 aatgttcttt cccgacatta tgttcatctt gagagatccc cttggaaagg acttccagaa
     4501 ctgaccaatg agaatgccca gtactgtcct ttcagctgtg aaacacaagc ctggtcaatt
     4561 gctactattc ttgagacact ttatgattta tagtttatta cagatattaa gtatgcaatt
     4621 actgtattat aggatgcaag gtcatcatat gtaaatgcta tatgcacagg ctcaagttgt
     4681 tttaaaaatc tcatttatta taatattgat gctcaattag gtaagattgt aaaagcattg
     4741 atttttttta atgtacagag gtagatttca atttgaatca gaaagaaata tcattaccaa
     4801 tgaaatgtgt ttgagttcag taagaattat tcaaatgcct agaaatccat agtttggaaa
     4861 ataaaaatca tgtcatcttc tatttgtaca gaaatgaaaa taaaatatga aaataatgaa
     4921 agaaatgaaa agatagcttt taattgtggt atatataatc ttcagtaaca atacatactg
     4981 aatacgctgt ggttcattaa tattaacacc acgtactata gtattcttag aatacagtgc
     5041 tcactgcatt taataaatat ttaataaatg atgaatgata gaagtttcca tctacaatat
     5101 atgttcctaa atggagcaca gatgttcaaa ctatgctttc attttttcac tgatatatta
     5161 atttttgtgt aatgaatgcc aacagtatat tttatatgat ttacttatgt gaggaaacat
     5221 gcaaagcatt aggaaattta tttcctaaaa acagttttgt aaaattagta ttgagttcta
     5281 ttgagtatta taagatagct tacattttca aaatggaaat tgtcggtcat atttctagaa
     5341 ctttaaagaa aaaagaatgt tatattagtt ttctaaaact caactatctt tagtcatgtt
     5401 caaaaatcta ttgctagatc atagtagata ctggttttct attaactcaa aacctacatt
     5461 gacaagttta acattgagaa gaatcttaac aaaaatatgg atatgaattc agtagatatc
     5521 ttaaattcaa taaaatcact ggaagttttt catgataact tattttaaga tgccttaaaa
     5581 atcttaaagt cacaaaagga aaaaggtttt taacatttac atgagttaac attttttcat
     5641 agaacttatt tcctagatag aattttttac tgttttttac tgttttctta agaaaacagt
     5701 taaatcatta tgcattcagt tggaagaaag tagtggcaag aattctttca ttgctatata
     5761 atattcagtg gctcatttat acctaataaa ataatggtat tttaaaataa tgctactttc
     5821 aaagtagcat ttttttagtt agtttacagg ttacataccc aaaaccttaa ctatgactaa
     5881 gaaattaaag aagaaaacca gcaaactaaa acttctgggc agcaaaaata tataaatgct
     5941 tcagatgtca aatacccatg cttgaaagct cgtgtaattt actttaagat tatctgcctg
     6001 ctcttcttca aagctgacct tgctttagaa atagttttaa ctagcttagt tttctggttt
     6061 ccaaaactaa aatagattaa atcctacaaa tttaaggaca gttgtgacag taatctgacc
     6121 actatctata aatacattgg acattggttt ccaaatctcc ctttctcttc agttccttcc
     6181 ttgttcaata tatacccttc tctaaactgt gcgggtaaaa ggaatgactg tccttgagag
     6241 aaccattagt ttatcaaagg tttatgtagt tttgttgctg taccctaact ttgatattca
     6301 gggaggtagg aaaggtaaca gaaaaccagc atatttaatc aaagcaagaa gtaatcgctg
     6361 acagttaaat gtgaccaaaa aaattaaaag ttcacaattt ttttaatgta gccatttggg
     6421 gttatctcta gtaaggcaga tacccacgtt ggtaaatttt taggatattg tgttgcacta
     6481 gaaaactaag tggttcatat ttctaatgag gaagattaat gaaagaacat tgttatattc
     6541 tgcgtggtat attttaaagt ttaagaaggc atgttaaaca ttatttcctc tatggtagtt
     6601 aaaatacaga attagatttt taacaggtgt catttgacta aacgtttcgg tagaatgctt
     6661 catacttgag tgatgctgga taaggtattg tatttcaaca atggactatg ccttggtttt
     6721 tcactaatca aaatcaaaat tactctttaa catgataaat gaatttacca gtttagtatg
     6781 ctgtggtatt ttaataagtt ttcaaagata attgggaaaa catgagactg gtcatattga
     6841 tgaatattgt aacatgtgaa ttgtgatcca tttctgatat gtcttgaact actgtgtcta
     6901 gtaggcaaat gtcattgtta cctctgtgtg ttaagaaaat aaaaatattt tctaaaggtc
     6961 g
//