From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Escherichia coli sodium-hydrogen antiporter (nhaB) gene, complete
Date: 22 Jul 92 10:01:33 GMT
Distribution: bionet

LOCUS       ECONHAB      2321 bp ds-DNA             BCT       22-JUL-1992
DEFINITION  Escherichia coli sodium-hydrogen antiporter (nhaB) gene, complete
            cds; fadR gene, 3' end; umuC gene, complete cds.
ACCESSION   M83655
KEYWORDS    Na/H antiporter; fadR gene; nhaB gene; sodium-hydrogen antiporter;
            umuC gene.
SOURCE      Escherichia coli (sub_strain W1333, strain K-12) DNA.
  ORGANISM  Escherichia coli
            Prokaryotae; Gram-negative facultatively anaerobic rods;
            Enterobacteriaceae.
REFERENCE   1  (sites)
  AUTHORS   Kitagawa,Y., Akaboshi,E., Shinagawa,H., Horii,T., Ogawa,H. and
            Kato,T.
  TITLE     Structural analysis of the umu operon required for inducible
            mutagenesis in Escherichia coli
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 82, 4336-4340 (1985)
  STANDARD  full staff_review
REFERENCE   2  (sites)
  AUTHORS   Dirusso,C.C.
  TITLE     Nucleotide sequence of the fadR gene, a multifunctional regulator
            of fatty acid metabolism in Escherichia coli
  JOURNAL   Nucleic Acids Res. 16, 7995-8009 (1988)
  STANDARD  full staff_review
REFERENCE   3  (bases 1 to 2321)
  AUTHORS   Pinner,E., Padan,E. and Schuldiner,S.
  TITLE     Cloning, sequencing and expression of the nhaB gene, encoding a
            Na+/H+ antiporter in Escherichia coli.
  JOURNAL   J. Biol. Chem. 267, 11064-11068 (1992)
  STANDARD  full staff_entry
FEATURES             Location/Qualifiers
     CDS             1..82
                     /gene="fadR"
                     /codon_start=2
                     /translation="RLHFPHLFPLDYQDLPHSCLTVKLCV"
                     /partial
     CDS             153..1667
                     /product="Na+/H+ antiporter"
                     /gene="nhaB"
                     /codon_start=1
                     /translation="MANFLGQSPDWYKLALIIFLIVNPLIFLISPFVAGWLLVAEFIF
                     TLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFMVAGIYFMKQ
                     LLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVGFYGIYHRVA
                     SSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTMVGEPQNLII
                     AKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKVREVLQQFDD
                     QSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSLTGVTDEHAI
                     GKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLFYIFNGLLSS
                     ISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPNGQAAFLFLL
                     TSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMGWIATL"
     terminator      1677..1699
                     /gene="nhaB"
     CDS             1764..1952
                     /note="putative ORF"
                     /codon_start=1
                     /translation="MNWFKLRTLCILQGNDYVAIFEPMFTRPGRVAVDGVYCSGTGTD
                     GAVVPACDVTETLRALYL"
     CDS             2032..2313
                     /gene="umuC"
                     /codon_start=1
                     /translation="MVIWLYSAFRGVQLTYEHTMLQLYPSPFATCDFMVRFPEWLPLD
                     KWVPQVFVASGDLRRASVGFFRSGNAAVAARYFYRLPDCRSAGGDFPAV"
BASE COUNT      436 a    588 c    613 g    684 t
ORIGIN      25.5 minutes
        1 aaggcttcat tttccgcatc tctttcccct tgactatcaa gacttgcctc actcctgcct
       61 gaccgttaag ctttgcgttt aaacataaaa acatcattga ttatttgtaa ggtaagggaa
      121 accatcatgg agatctcctg gggccgcgcg ctatggcgaa ttttttgggc cagtcccccg
      181 actggtacaa actcgccctc atcattttct taatcgtaaa cccgttaatt ttcctcatca
      241 gccctttcgt cgcgggctgg ttgctggtag cggaatttat tttcactctg gcgatggccc
      301 tgaaatgcta cccgctgctc cccggtggtc tgttggctat cgaagcggta ttcatcggca
      361 tgaccagcgc ggaacacgtc cgtgaagagg tggcggcaaa tcttgaagtc ttgctgttac
      421 tgatgtttat ggtggcgggt atctatttta tgaaacagct gttgctgttc atatttaccc
      481 gtttgctgtt aagcattcgc tccaaaatgc tgctgtcgct ctctttttgc gtggcggctg
      541 cgttcctctc cgcgttcctc gatgccttaa ccgtcgtggc ggtggtgatc agcgttgcag
      601 tcggttttta tggtatttat caccgcgtag cctcttcccg taccgaagac accgacctgc
      661 aagacgatag tcatatcgac aagcattaca aagtggttct ggaacagttc cgtggctttc
      721 tgcgtagcct gatgatgcat gccggtgtcg gcaccgcatt aggcggcgta atgaccatgg
      781 tgggcgaacc acagaacctg atcatcgcta aagcggctgg ctggcatttt ggcgatttct
      841 tcctgcgcat gtcgccggtg accgttccgg ttctgatttg tggcctgtta acctgcctgc
      901 tggtagagaa gctgcgttgg tttggctacg gtgaaacgct gccggagaaa gtccgcgaag
      961 tgttgcaaca gtttgacgat caaagccgcc accagcgtac ccgtcaggat aaaatccgtc
     1021 tgattgtcca ggcgattatt ggcgtctggc tggtgactgc gctggcgttg catctggcgg
     1081 aagttggctt gattggtttg tcagtcatta ttctggcaac atcattgacc ggtgtcaccg
     1141 atgagcatgc tatcggtaaa gccttcaccg aatctctgcc attcaccgca ctgttgacgg
     1201 tgtttttctc ggtagtcgcg gtgattatcg accaacaact gttttcgcca attattcagt
     1261 ttgtgttgca ggcatcggaa catgctcagc tgtcgctgtt ctatattttc aacggtctgc
     1321 tgtcatccat ttcggataac gtcttcgtgg ggacgattta tatcaacgaa gcgaaagcgg
     1381 caatggaaag tggcgctatc acgttgaagc aatacgagct gctggcggtc gccattaata
     1441 ccggtaccaa tctgccctcc gtcgctacgc cgaacggtca ggctgcgttc ctgttcctgc
     1501 tgacctctgc actcgcgcca ttgattcgcc tctcttatgg ccgcatggtg tggatggccc
     1561 tgccttacac cctcgtcctg acactcgtcg gcttgctctg cgtcgagttt acgcttgccc
     1621 ctgtaaccga atggtttatg caaatgggct ggatagcaac gctttgataa caacttaccg
     1681 ggcatataaa tgcccggttt gccttttcgc ccgataattg tccaattgcg tcctattttt
     1741 gctgcctcct ggtggcgcag cgaatgaatt ggtttaaact gcgcactcta tgcatattgc
     1801 agggaaatga ttatgttgcg atttttgaac caatgttcac aaggccgggg cgcgtggctg
     1861 ttgatggcgt ttactgctct ggcactggaa ctgacggcgc tgtggttcca gcatgtgatg
     1921 ttactgaaac cttgcgtgct ctgtatttat gaacgctgcg cgttattcgg cgttctgggt
     1981 gctgcgctga ttggcgcgat cgccccgaaa actccgctgc gttatgtagc gatggttatc
     2041 tggttgtata gtgcgttccg cggtgtgcag ttaacttacg agcacaccat gcttcagctc
     2101 tatccttcgc cgtttgccac ctgtgatttt atggttcgtt tcccggaatg gctgccgctg
     2161 gataagtggg tgccgcaagt gtttgtcgcc tctggcgact tgcgccgagc gtcagtggga
     2221 ttttttaggt ctggaaatgc cgcagtggct gctcggtatt tttatcgctt acctgattgt
     2281 cgcagtgctg gtggtgattt cccagccgtt taaagcgaaa a
//