From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: V.parahaemolyticus nutA gene for 5'-nucleotidase, complete cds.
Date: 13 Aug 92 10:01:15 GMT
Distribution: bionet
Checksum: 12923     6

LOCUS       VIBNUTA      1956 bp ds-DNA             BCT       13-AUG-1992
DEFINITION  V.parahaemolyticus nutA gene for 5'-nucleotidase, complete cds.
ACCESSION   D00910
KEYWORDS    5'-nucleotidase; Cl--dependent 5' -nucleotidase;
            membrane bound 5'-nucleotidase.
SOURCE      V.parahaemolyticus (strain AQ3334) DNA, clone pNUT54.
  ORGANISM  Vibrio parahaemolyticus
            Prokaryotae; Facultatively anaerobic gram-negative rods;
            Vibrionaceae.
REFERENCE   1  (bases 1 to 1956)
  AUTHORS   Tamao,Y., Noguchi,K., Sakai-Tomita,Y., Hama,H., Shimamoto,T.,
            Kanazawa,H., Tsuda,M. and Tsuchiya,T.
  TITLE     Sequence analysis of nutA gene encoding membrane-bound
            Cl--dependent 5'-nucleotidase of Vibrio parahaemolyticus
  JOURNAL   J. Biochem. 109, 24-29 (1991)
  STANDARD  simple staff_entry
COMMENT     These data kindly submitted in computer readable form by:
            Yumi Tamao
            Department of Microbiology
            Faculty of Pharmaceutical Science
            Okayama University
            Tsushima, Okayama 700
            Japan
            Phone:  0862-52-1111 x992
            Fax:    0862-55-7456
FEATURES             Location/Qualifiers
     -35_signal      117..122
                     /note="putative -35 region A"
     -35_signal      131..136
                     /note="putative -35 region B"
     -10_signal      140..145
                     /note="putative -10 region A"
     -10_signal      156..161
                     /note="putative -10 region B"
     repeat_unit     159..165
                     /note="dyad symmetry 1"
     repeat_unit     170..179
                     /note="dyad symmetry 2"
     repeat_unit     187..196
                     /note="dyad symmetry 2"
     repeat_unit     198..204
                     /note="dyad symmetry 1"
     RBS             224..228
     CDS             234..1916
                     /note="5'-nucleotidase precursor"
                     /codon_start=1
                     /translation="MNQRLIIKTALSAAILASLAGCASQPAHEWNADTTYKLTVLHTN
                     DHHGRFWQNKHGEYGMAARKTLIDDLRDEIQAEGGSVLLLSGGDINTGVPESDLQDAE
                     PDFKGMSKIGYDAMALGNHEFDNPLDVLFKQQDWANFPMLSANIYDKKTGKRLFQPYA
                     MFNKQGIKIAVIGLTTEDTAKLGNPEFIGQVDFRDPKAEAKELIAELKKTENPDLIFA
                     VTHMGHYENGNRGINAPGDVALARYLNEGDLDMIVGGHSQEPVCMEGPNVIKKNFKPG
                     DECQPDQQNGTYIVQAHEWGKYVGRADYEFRNGELSMVSYDLIPVNLKKKINVDGQSQ
                     RVFVQDEITQDKAMLDFLRPFQEKGQSQLNVKIAESNGKLEGDRDVVRFQQTNLGRLI
                     ATAHMERAKADFAVMNSGGVRDSIEAGDITYKDVLTVQPFGNMVSYVDMSGQEVLDYL
                     NIVATKPVDSGAYAQFAGISMRIENDKVTNVFIGNKQLRLDGRYRFTVPSYNASGGDG
                     YPKIDTHPGYVNTGFTDAEVLKDYLESHSPIDVNEYAPSGEVMYQTNNVVNQ"
     sig_peptide     234..296
                     /note="5'-nucleotidase signal peptide"
                     /codon_start=1
     mat_peptide     297..1913
                     /note="5'-nucleotidase mature peptide"
                     /codon_start=1
     repeat_unit     1936..1940
                     /note="dyad symmetry 3"
     repeat_unit     1944..1948
                     /note="dyad symmetry 3"
BASE COUNT      596 a    390 c    461 g    509 t
ORIGIN
        1 atcaagtaat gaattgttac tgttcatgat gaaaaataaa atagccaacc tagagttggc
       61 tattttttta tctgataaac cggtaaattc tcaaacttgt tactgatgaa tttgttattg
      121 ctaaacaatg catcataatt atgaaatcaa tgtgttaggg tgcgcgtggt tttcaaacag
      181 ctctaatgtt tgaaaatgtg catcagtaat cccgataaga aaaaggaaaa aaaatgaatc
      241 agcgtctgat tatcaaaacc gctttgagtg ccgctatttt ggcctcgctg gctggatgtg
      301 cttcgcaacc tgctcatgaa tggaatgcag ataccactta taaattgact gtcttacaca
      361 ccaacgatca ccacggtcgt ttttggcaaa ataaacacgg tgagtacggc atggccgcgc
      421 gcaagacgtt gatcgatgat ctgcgtgatg aaattcaagc agaaggaggc agcgtgctgt
      481 tgctgtctgg tggtgatatt aataccggcg ttcctgaatc tgatttacaa gacgctgaac
      541 cagatttcaa aggcatgagt aagattggct acgacgcgat ggcgttaggc aatcacgaat
      601 ttgataatcc gctagatgtg ctattcaaac agcaagattg ggctaacttc ccaatgctgt
      661 cagccaacat ctacgacaaa aaaacgggca agcgtttatt ccaaccttac gcgatgttca
      721 ataaacaagg cataaaaatt gcggttattg gtctgacaac agaagatacc gcgaagttgg
      781 gaaatcctga gtttatcggt caagtggact tccgcgaccc gaaagcggaa gcgaaagagc
      841 tgattgcaga actgaaaaaa acagaaaatc cggatctgat tttcgctgtt acgcacatgg
      901 gtcactacga aaatggcaac cgtggcatca atgcgccggg tgatgtagct ttggcgcgtt
      961 accttaatga aggcgatttg gacatgattg tgggtggtca ctcacaagag cccgtttgta
     1021 tggaaggtcc taacgttatc aaaaagaact ttaaaccggg tgatgagtgt cagccagacc
     1081 aacaaaacgg tacttacatt gttcaggcgc atgagtgggg taaatatgtt ggccgtgccg
     1141 attacgaatt ccgtaacggc gaactatcaa tggtgagcta cgatcttatt ccggtaaact
     1201 tgaagaaaaa aatcaacgtg gatggtcaat cacaacgtgt gtttgttcaa gatgaaatta
     1261 ctcaagacaa agcaatgttg gacttccttc gtcctttcca agaaaaaggc caaagccagc
     1321 tgaatgtgaa aattgcggaa tcaaacggta agctagaagg tgaccgtgat gtcgtgcgat
     1381 tccaacagac gaatctaggt cgtttaattg caacggctca tatggaacgt gcaaaagcag
     1441 atttcgcagt gatgaactca ggcggtgtgc gtgattctat tgaagctggt gacatcacat
     1501 acaaagatgt gctgaccgtg cagccatttg gcaacatggt ttcttacgtc gatatgtctg
     1561 ggcaagaagt cttggattac ctaaacattg ttgcaaccaa gccagttgat tcaggtgctt
     1621 acgcgcagtt tgcgggtatc tctatgcgta ttgaaaatga caaagtcacc aatgtattta
     1681 tcggtaacaa gcaactgcgt ttagatggtc gttaccgctt cacagtacca agttacaacg
     1741 cttctggtgg cgatggttac cctaaaattg atacgcaccc tggctacgtc aacacaggtt
     1801 ttaccgatgc agaagtcttg aaagactatc tagaaagcca tagcccaatt gacgttaacg
     1861 aatacgcacc ttctggtgag gtgatgtacc aaacaaacaa tgtggtcaat caatagagtt
     1921 tgattgcgaa aaagcgtttg actcaaactc gctgat
//