From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Psophocarpus tetragonolobus DNA for WCI-3.
Date: 6 Jan 93 07:02:04 GMT
Distribution: bionet
Checksum: 11198 5

LOCUS       PSTWCI3B     1873 bp ds-DNA             PLN       06-JAN-1993
DEFINITION  Psophocarpus tetragonolobus DNA for WCI-3.
ACCESSION   D13976
KEYWORDS    WCI-3.
SOURCE      Psophocarpus tetragonolobus (cultivar ishigaki4, strain (L.)DC.)
            Mature Leaf DNA, clone pWBG-7.
  ORGANISM  Psophocarpus tetragonolobus
            Eukaryota; Plantae; Embryobionta; Magnoliophyta; Magnoliopsida;
            Rosidae; Fabales; Fabaceae.
REFERENCE   1  (bases 1 to 1873)
  AUTHORS   Habu,Y., Peyachokunagul,S., Umemoto,K., Sakata,Y. and Ohno,T.
  TITLE     Structure and regulated expression of Kunitz chymotrypsin inhibitor
            genes in winged bean (Psophocarpus tetragonolobus (L.) DC.)
  JOURNAL   J. Biochem. 111, 249-258 (1992)
  STANDARD  full automatic
REFERENCE   2  (bases 1 to 1873)
  AUTHORS   Habu,Y., Sakata,Y., Fukasawa,K. and Ohno,T.
  TITLE     Ubiquitous nuclear proteins bind to multiple sites in 5' upstream
            region of major Kunitz chymotrypsin inhibitor gene in winged bean
            (Psophocarpus tetragonolobus (L.) DC.)
  JOURNAL   Unpublished (2048)
  STANDARD  full automatic
COMMENT     Submitted (24-DEC-1992) to DDBJ by: Yoshiki Habu Department of
            Applied Bioscience Faculty of Agriculture Hokkaido University Nishi
            9, Kita 9 Kita-ku, Sapporo Hokkaido 060 Japan Phone:  011-716-2111
            x3887 Email:  yoshiki_habu%al%hines@xmail-gw.sys.hokudai.ac.jp Fax:
               011-746-0208.
FEATURES             Location/Qualifiers
     TATA_signal     1042..1048
     mRNA            1077..1851
     5'UTR           1077..1116
     sig_peptide     1117..1188
                     /codon_start=1
     mat_peptide     1189..1737
                     /product="WCI-3"
                     /codon_start=1
     3'UTR           1741..1851
     polyA_signal    1798..1803
     polyA_signal    1818..1823
     CDS             1117..1740
                     /standard_name="winged bean major Kunitz chymotrypsin
                     inhibitor gene B"
                     /product="WCI-3"
                     /codon_start=1
                     /translation="MKSTTFLALFLLSAIISHLPSSTADDDLVDAEGNLVENGGTYYL
                     LPHIWAHGGGIETAKTGNEPCPLTVVRSPNEVSKGEPIRISSQFLSLFIPRGSLVALG
                     FANPPSCAASPWWTVVDSPQGPAVKLSQQKLPEKDILVFKFEKVSHSNIHVYKLLYCQ
                     HDEEDVKCDQYIGIHRDRNGNRRLVVTEENPLELVLLKAKSETASSH"
BASE COUNT      570 a    305 c    290 g    708 t
ORIGIN      
        1 tctctccacc tttctcaata agaacatttt tacaattatt taaaatataa aaaattatta
       61 ttaaatgtca aaagattcaa ttttcgtatc tcctttcttt cttttctaat taaaaaatat
      121 gagtcacgct ttattttttt tatttattat gtaagtcata aatgataaag cattttttat
      181 taatggaaca aaaatgcaag attttttcgt ctatattata tgcccatcta cattttattt
      241 tttctatcta ttattttttt tctataattc tatcttatat taattattat attaataaat
      301 attttctctc ttgtgatgtc taataacatg tttaggtgtc ctaaaatttt ttaataaata
      361 tatcaagttc acgtacagta tatttaaact acctagatat ttattgaaat ttatttttgg
      421 caatctcttt attttatttt tggtaaagtc tttctttctt ctggattttc cattgagttt
      481 tggtacgttt tatattttgt tcatctccat tatgagtgga gtcatttggt ttgtgtcacg
      541 actatattta tcagtacatg cattggctag attgacggta tattatgtca tgacttgagt
      601 cttgaggctt gttggttgtg tattacgttt catgggtaca cgtatagata agatttgaag
      661 gccaattttg ttgtgaacga taaaagggta tctctatatg cataaaatag ctttaataac
      721 tgttttccta gaaaatagca ttttagactt tttgttttat gttagtgttt aaacatgttt
      781 attttatttt agttctatga gctgctgtaa tttttcatgt aatgcaaagg agtcttaaac
      841 attagaggat aaactggtat aattttcttt tttctataaa ttatatttta atcacttata
      901 agctagtttc atgtctaaat tataatgcat taataagaaa ttaaaaacaa agttttgtct
      961 aaaaatttat accagactat ttacaaattc gtttaattaa taatgttatc gaaaacacag
     1021 tttatagtta acgagcactt ctataaatag tctacattcg gcgaaaccta ttctctcaaa
     1081 tgagtagaaa ggaaatcgtt ccacaagcat aaaaaaatga agagtactac atttcttgcc
     1141 ctctttctac tctctgccat catctcacac ctaccatcat ccactgctga tgatgatttg
     1201 gtcgatgctg aaggtaactt agttgaaaat ggtggcacat actatctgtt gccacatata
     1261 tgggcacacg ggggaggaat agaaacagca aaaacaggaa acgaaccatg ccctctaaca
     1321 gtggtacgat ctcccaatga ggtctctaaa ggggaaccaa taaggatctc atcccaattc
     1381 ctttcattgt tcatccccag aggctctcta gtggctcttg gattcgctaa ccctccatct
     1441 tgtgcagctt ctccgtggtg gactgttgtt gactctccac aaggacccgc tgttaaactt
     1501 agtcagcaaa aacttccgga aaaggatatt ctagtgttta aattcgagaa agtttcccat
     1561 tctaacattc acgtgtacaa gcttttgtac tgtcaacatg acgaagagga tgtgaagtgt
     1621 gatcagtata tcgggattca tagggatcgc aatggaaaca gacgtttggt ggtgactgag
     1681 gaaaacccat tagagcttgt gcttctgaaa gctaagtcag aaactgcatc aagccattaa
     1741 gcacttctca tccttaaaag tcatctgtgc ttatgactaa atgaatgctt tgcttgtaat
     1801 aaattgtcac accactcaat aaaattatct aagtttttgc ttttcttttt tatttttgtg
     1861 cttttaagaa ttc
//