From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: aliphatic nitrilase [Rhodococcus rhodochrous, K22, Genomic, 1653
Date: 3 Jan 93 22:20:55 GMT
Distribution: bionet
Checksum: 00990 4

LOCUS       S114184      1653 bp    DNA             BCT       24-NOV-1992
DEFINITION  aliphatic nitrilase [Rhodococcus rhodochrous, K22, Genomic, 1653
            nt]
ACCESSION   S45800 D12583
KEYWORDS    .
SOURCE      Rhodococcus rhodochrous K22
  ORGANISM  Rhodococcus rhodochrous
            Unclassified.
REFERENCE   1  (bases 1 to 1653)
  AUTHORS   Kobayashi,M., Yanaka,N., Nagasawa,T. and Yamada,H.
  TITLE     Primary structure of an aliphatic nitrile-degrading enzyme,
            aliphatic nitrilase, from Rhodococcus rhodochrous K22 and
            expression of its gene and identification of its active site
            residue.
  JOURNAL   Biochemistry 31, 9000-9007 (1992)
  STANDARD  full automatic
COMMENT     This entry [NCBI gibbsq 114184] was created by the journal scanning
            component of NCBI/GenBank at the National Library of Medicine.
            This sequence comes from Fig. 3.
FEATURES             Location/Qualifiers
     CDS             314..1465
                     /note="For the protein sequence (NCBI gibbsq 114185):
                     Method: conceptual translation with partial peptide
                     sequencing.  This sequence comes from Fig. 3."
                     /product="aliphatic nitrilase"
                     /codon_start=1
                     /translation="MSSNPELKYTGKVKVATVQAEPVILDADATIDKAIGFIEEAAKN
                     GAEFLAFPEVWIPGYPYWAWIGDVKWAVSDFIPKYHENSLTLGDDRMRRLQLAARQNN
                     IALVMGYSEKDGASRYLSQVFIDQNGDIVANRRKLKPTHVERTIYGEGNGTDFLTHDF
                     GFGRVGGLNCWEHFQPLSKYMMYSLNEQIHVASWPAMFALTPDVHQLSVEANDTVTRS
                     YAIEGQTFVLASTHVIGKATQDLFAGDDDAKRALLPLGQGWARIYGPDGKSLAEPLPE
                     DAEGLLYAELDLEQIILAKAAADPAGHYSRPDVLSLKIDTRNHTPVQYITADGRTSLN
                     SNSRVENYRLHQLADIEKYENAEAATLPLDAPAPAPAPEQKSGRAKAEA"
BASE COUNT      340 a    586 c    467 g    260 t
ORIGIN      
        1 ggatccaccc cggtcgcggt gacctcgcgc cgattgggat gacgtttgtc attggttgat
       61 ctgccgttga gagacttcgg agcgggtgat gagtaacttt ggtcacagcg cagaaagcga
      121 agtcctcgaa gtccagaaca tcaacagcaa caacgcctct cacgtcgtat cgctgtgttc
      181 gagcacgtct cgactgcaga cctcaacgtc gacaccggtg gtggctgatc gtttttagcg
      241 cactgaagga actcgccgaa accctcggca tcactccaca catcgcatca ctccacacat
      301 caaggagcac agcatgtcca gcaatccaga gctcaagtac accggcaagg taaaggtggc
      361 caccgtccag gccgagccgg tcatcctcga tgccgacgcg accatcgaca aggctatcgg
      421 attcatcgag gaggccgcca agaacggtgc cgagttcctc gcattccccg aagtctggat
      481 ccccggttac ccgtactggg catggatcgg cgacgtcaag tgggccgtca gcgacttcat
      541 cccgaagtac catgagaact ccctcaccct cggcgacgac cgcatgcggc gcctgcagct
      601 ggccgcgcgg cagaacaaca ttgccctggt gatgggctac tccgagaagg acggcgccag
      661 ccgctacctc tcgcaggtct tcatcgacca gaacggtgac atcgtcgcca accggcgcaa
      721 gctcaagccg actcacgtcg aacgcaccat ttacggcgag ggcaacggta ccgacttcct
      781 cacccacgac ttcgggttcg gtcgtgtcgg cggcctgaat tgctgggagc acttccagcc
      841 gctgagcaag tacatgatgt acagcctcaa cgagcagatt cacgtcgcgt cgtggccggc
      901 gatgttcgct ctcacccccg acgtccacca gctcagcgtg gaagccaacg acaccgtcac
      961 ccgctcgtac gcgatcgagg gccagacctt cgtgctcgcc tcgacacacg tgatcggcaa
     1021 ggccacccag gatctgttcg ccggagacga cgacgccaag cgggcgctgt tgcctctcgg
     1081 ccagggttgg gcccgcatct acggtcccga cggcaagtca ctggccgaac cgctgcccga
     1141 ggacgccgaa ggcctgctct acgccgaact cgacctcgag cagatcatcc tggccaaggc
     1201 cgctgcggac ccggcgggtc actactcccg gcccgacgtc ctctcactca agatcgacac
     1261 ccgcaaccac acgcccgtcc aatacatcac cgcggacggc cgaacctcgc tcaactccaa
     1321 cagccgggtc gagaactacc ggctgcacca gctcgccgac atcgagaagt atgagaacgc
     1381 cgaggccgcc acgctccccc tcgacgcacc cgcacccgca cccgcacccg agcagaagtc
     1441 cggcagggcc aaggcggagg cctagccccc ggtggttgcc gcctgatcga aggcgcgagc
     1501 atctgcgccg acggacgact gtcgtccttc cttactccct ggacgcccgc agcaatggcg
     1561 cgggcgtcca gggactccag gtcactgcgg gcatctcgca cccaatgctg aggcggcgcc
     1621 gtcggcaccc actctcgacc gaaaggaggg ccc
//