From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: E.coli K-12 ORF294 for putative polypeptide 294, tRNA-lys, tRNA-val
Date: 15 Sep 92 10:02:56 GMT
Distribution: bionet
Checksum: 22251     5

LOCUS       ECOGLTXU     1880 bp ds-DNA             BCT       15-SEP-1992
DEFINITION  E.coli K-12 ORF294 for putative polypeptide 294, tRNA-lys, tRNA-val
ACCESSION   X63976 X55757
KEYWORDS    gltX gene.
SOURCE      Escherichia coli DNA.
  ORGANISM  Escherichia coli
            Prokaryotae; Gram-negative facultatively anaerobic rods;
            Enterobacteriaceae.
REFERENCE   1  (bases 1 to 1880)
  AUTHORS   Brun,Y.V., Breton,R., Lanouette,P. and Lapointe,J.
  TITLE     Precise mapping and comparison of two evolutionarily related
            regions of the Escherichia coli K-12 chromosome.
  JOURNAL   J. Mol. Biol. 214, 825-843 (1990)
  STANDARD  full automatic
COMMENT     *source: strain=K-12; *source: sub_clone=pLQ7610, pLQ7613;
            From EMBL    entry ECGLTXU;  dated 01-JUL-1992.
FEATURES             Location/Qualifiers
     -35_signal      12..17
     -10_signal      36..41
     RBS             57..63
     CDS             73..957
                     /gene="ORF294"
                     /codon_start=1
                     /translation="MERVYRTDLKLLRYFLAVAEELHFGRAAARLNMSQPPLSIHIKE
                     LENQLGTQLFIRHSRSVVLTHAGKILMEESRRLLVNANNVLARIEQIGRGEAGRIELG
                     VVGTAMWGRMRPVMRRFLRENPNVDVLFREKMPAMQMALLERRELDAGIWRMATEPPT
                     GFTSLRLHESAFLVAMPEEHHLSSFSTVPLEALRDEYFVTMPPVYTDWDFLQRVCQQV
                     GFSPVVIREVNEPQTVLAMVSMGIGITLIADSYAQMNWPGVIFRPLKQRIPADLYIVY
                     ETQQVTPAMVKLLAALTQ"
     terminator      1035..1061
     terminator      1191..1216
     tRNA            complement(1221..1296)
                     /product="lysV"
     tRNA            complement(1301..1376)
                     /product="valU (gamma)"
     tRNA            complement(1423..1499)
                     /product="valU (beta)"
     tRNA            complement(1544..1619)
                     /product="valU (alpha)"
     CDS             1878..>1880
                     /gene="gltX"
                     /codon_start=1
                     /translation="M"
BASE COUNT      466 a    433 c    496 g    485 t
ORIGIN
        1 gatatctggt ggtgaaatac tttatgccat gataatttaa tacgatgtat ttattatatg
       61 gagcacttaa ttatggaacg cgtatacaga acagatctta agttgctccg ttattttctt
      121 gccgtagcgg aagagttgca ttttggccgc gcagcagcgc gtttaaatat gtctcagcct
      181 ccgctcagca ttcatattaa agagctggaa aatcaactcg gcacgcagct ttttattcgc
      241 cattcgcgca gcgtcgtact gacacacgcg ggcaaaatct tgatggaaga atcgcgtcga
      301 ttgctggtga atgctaataa tgtattggct cggattgaac aaataggtcg gggagaagca
      361 gggcggattg aactcggcgt agtgggaacg gcaatgtggg gacggatgcg cccggttatg
      421 cggcgattcc tcagggaaaa tcctaacgtt gacgttcttt ttcgcgaaaa gatgcccgcg
      481 atgcaaatgg ccttgctgga acgccgcgaa cttgatgccg ggatctggcg aatggcgaca
      541 gaaccaccga ctggttttac cagcttacgg ttgcatgaat cggcgtttct ggtggcgatg
      601 cctgaagagc atcatctctc atcattttcc accgtcccgc tggaagcgct acgtgacgag
      661 tattttgtta caatgccgcc cgtttacact gactgggatt ttttgcagcg agtttgccag
      721 caggtgggat tttcaccggt tgttattcgc gaagttaatg aaccgcaaac ggtactcgct
      781 atggtcagta tgggcattgg tatcacattg atagcggaca gctacgcaca aatgaactgg
      841 ccaggtgtca ttttccgtcc gctcaaacag cgcatccctg cagatttata tattgtttat
      901 gaaacacagc aggtgacgcc cgcgatggtt aagctgttgg ctgcattgac acagtaggtg
      961 aggagacagg aattgccgga aatagtagtc tgctaagtga agcgcatcag gcaatttcgc
     1021 aaatcgcaga tagcaaaaaa cggcctttag ggcgcttttt tacattggtg ggtcgtgcag
     1081 gatgactcgc ttcgctcgcc cttcgggccg tcgccgcgag cggctccgtt gtttcgctgc
     1141 gctcaactca aacctgctgc aggttcgaat ctttgagtat cgcagatagt aaaaaagcgc
     1201 ctttagggcg ttttttacat tggtgggtcg tgcaggattc gaacctgcga ccaattgatt
     1261 aaaagtcaac tgctctacca actgagctaa cgacccgaag tggtgggtga tgacgggatc
     1321 gaaccgccga ccccctcctt gtaagggagg tgctctccca gctgagctaa tcacccactt
     1381 cggtacttca catttttaca agaaatttag ctggcgagaa agtggtgggt gatgacggga
     1441 tcgaagccgc cgaccccctc cttgtaaggg aggtgctctc ccagctgagc taatcaccca
     1501 cttctcaatt tcttgctaca cggcggagac tacataaagt agttggtggg tgatgacggg
     1561 atcgaaccgc cgaccccctc cttgtaaggg aggtgctctc ccagctgagc taatcacccc
     1621 cgctgtgtgg agtcgcatta tagggagagt tcaaaatgag tcaacgcatt ttctaaagaa
     1681 attgttcgtt cgtcgtaaat ttaagcaaga tgatcgcaaa acagaccgtg ttgcgcaatt
     1741 tgtcaacgaa aacaataatg cgtaaggtag aaacccgaac tacattgagg aatcaggcgg
     1801 gagtgataga atatcgccca cttaattttt ccaggatttg ccggttgtcg gcatctttct
     1861 aaacgtaagg ccatttcatg
//