From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Bacillus subtillis membrane bound protein (lytA and lytR); amidase
Date: 31 Dec 92 01:43:06 GMT
Distribution: bionet
Checksum: 09408 12

LOCUS       BACLYTABCD   5280 bp ds-DNA             BCT       18-DEC-1992
DEFINITION  Bacillus subtillis membrane bound protein (lytA and lytR); amidase
            enhancer (lytB); and amidase (lytC) genes, complete cds's.
ACCESSION   M87645
KEYWORDS    amidase; amidase enhancer; cell wall binding protein; lytA gene;
            lytB gene; lytC gene; lytR gene; membrane bound protein.
SOURCE      Bacillus subtilis (strain 168) DNA.
  ORGANISM  Bacillus subtilis
            Prokaryotae; Firmicutes; Endospore-forming rods and cocci;
            Bacillaceae.
REFERENCE   1  (bases 1 to 5280)
  AUTHORS   Lazarevic,V.T., Margot,P., Soldo,B. and Karamata,D.
  TITLE     Sequencing and analysis of the Bacillus subtilis lytRABC divergon:
            a regulatory unit encompassing the structural genes of the
            N-acetylmuramoyl-L-alanine amidase and its modifier
  JOURNAL   J. Gen. Microbiol. 138, 1949-1961 (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     terminator      complement(52..88)
                     /gene="lytR"
     RBS             1019..1024
                     /gene="lytR"
                     /note="putative"
     -10_signal      complement(1043..1048)
                     /gene="lytR"
     -35_signal      complement(1066..1071)
                     /gene="lytR"
     -35_signal      1131..1136
                     /gene="lytA"
                     /note="standard name is sigA"
     -35_signal      1149..1153
                     /gene="lytB"
                     /note="standard name is sigD"
     -10_signal      1155..1160
                     /gene="lytA"
                     /note="standard name is sigA"
     -10_signal      1169..1175
                     /gene="lytB"
                     /note="standard name sigD"
     RBS             1181..1190
                     /gene="lytA"
     RBS             1512..1516
                     /gene="lytB"
     RBS             3670..3676
                     /gene="lytC"
     terminator      5178..5211
     CDS             complement(92..1012)
                     /gene="lytR"
                     /product="membrane bound protein"
                     /codon_start=1
                     /translation="MRNERRKKKKTLLLTILTIIGLLVLGTGGYAYYLWHKAASTVAS
                     IHESIDKSKKRDKEVSINKKDPFSVLIMGVDERDGDKGRADTLIYMTVNPKTNTTDMV
                     SIPRDTYTKIIGKGTMDKINHSYAFGGTQMTVDTVENFLDVPVDYFVKVNMESFRDVV
                     DTLGGITVNSTFAFSYDGYSFGKGEITLNGKEALAYTRMRKEDPRGDFGRQDRQRQVI
                     QGIINKGANISSITKFGDMFKVVENNVKTNLTFDNMWDIQSDYKGARKHIKQHELKGT
                     GTKINGIYYYQADESALSDITKELKESLEK"
     CDS             1196..1504
                     /gene="lytA"
                     /product="membrane bound protein"
                     /codon_start=1
                     /translation="MKKFIALLFFILLLSGCGVNSQKSQGEDVSPDSNIETKEGTYVG
                     LADTHTIEVTVDNEPVSLDITEESTSDLDKFNSGDKVTITYEKNDEGQLLLKDIERAN
                     "
     CDS             1528..3645
                     /function="cell wall binding protein"
                     /gene="lytB"
                     /product="amidase enhancer"
                     /codon_start=1
                     /translation="MKSCKQLIVCSLAAILLLIPSVSFAADSNISVKLLNYIGNKSSI
                     SLSPTGFYKVTGDNVAVTDRFAGASRYETATLASNSQWKNPNTVILVNRDIFIDALPV
                     IPLAKKLNAPVLFTQPDTLTKTTERQIAKFNPDNILIIGGARSISKDVENKLKSYGAV
                     KRISGKNRYVLSENIAKQMGSYDKAIVVTGRVFQDALAIAPYAAAHGYPILLTEKDKL
                     PDYDLPKQVIIIGSSFSVSDSVENQIKKTSTVQRIPGSTRYELTANIIKQLKLKADKV
                     VMTNGTKYADVLIGASLASKKNSQILFVKQDSVPAAAKSITKDKATYAYDFIGSTSSI
                     SAEVENSLADEFYLADGGTYNLKINSGKLNLENIKTYGNSLRIKPENYSTSNRISLDG
                     KQYLGTVNFSIESTKYIRPVNENIPFEDYLKGVIPNEMPASWSLEALKAQTVAARTYS
                     ITKTGTTVPDTTAFQVYGGYSWNSNTNKAVEQTKGKVLKYNGSLITAAYSSSNGGYTE
                     ASNEVWSSSVPYLIAKKDTKDPQIGWTLTLSKQQLDTKSLDLTKPSSWWSSATETDSA
                     RLSGVKNWILKNKETSADSVKIASIDDLSFSGTTQGQRAKTASMKVKYFVKSSTGSYN
                     LSKITTISVPTSELRTMIGATVFKSTYVTVKKDTSKYTISGKGYGHGIGMSQYGAKAR
                     AEAGDSYSSILKFYYPGTTLTSY"
     CDS             3684..5174
                     /function="cell wall binding protein"
                     /gene="lytC"
                     /product="amidase"
                     /codon_start=1
                     /translation="MRSYIKVLTMCFLGLILFVPTALADNSVKRVGGSNRYGTAVQIS
                     KQMYSTASTAVIVGGSSYADAISAAPLAYQKNAPLLYTNSDKLSYETKTRLKEMQTKN
                     VIIVGGTPAVSSNTANQIKSLGISIKRIAGSNRYDTAARVAKAMGATSKAVILNGFLY
                     ADAPAVIPYAAKNGYPILFTNKTSINSATTSVIKDKGISSTVVVGGTGSISNTVYNKL
                     PSPTRISGSNRYELAANIVQKLNLSTSTVYVSNGFSYPDSIAGATLAAKKKQSLILTN
                     GENLSTGARKIIGSKNMSNFMIIGNTPAVSTKVANQLKNPVVGETIFIDPGHGDQDSG
                     AIGNGLLEKEVNLDIAKRVNTKLNASGALPVLSRSNDTFYSLQERVNKAASAQADLFL
                     SIHANANDSSSPNGSETYYDTTYQAANSKRLAEQIQPKLAANLGTRDRGVKTAAFYVI
                     KYSKMPSVLVETAFITNASDASKLKQAVYKDKAAQAIHDGTVSYYR"
BASE COUNT     1751 a    951 c   1040 g   1538 t
ORIGIN      
        1 gtttcattac gggtatcgaa aagaacaacc ggattcattt acaggcaaat aaaaaagaag
       61 ctttgcacat tgtgcgaagc ttctttttgt tttacttttc gaggctttcc ttcagctctt
      121 ttgtaatatc ggaaagggcg ctttcatcag cttggtagta gtaaattcca ttgattttcg
      181 tgcctgtacc ttttagttcg tgctgtttaa tatgtttccg cgcacctttg taatcggact
      241 ggatatccca catgttatcg aaggtcaagt tcgttttcac attattttca acaactttaa
      301 acatatctcc gaatttcgtg atggaagaaa tattagcgcc tttattaata ataccttgga
      361 tgacttggcg ctggcggtcc tggcgtccga agtcgcctct cggatcttct tttctcatcc
      421 gggtataagc gagcgcttct tttccgttca gcgtgatttc tcctttgccg aacgaataac
      481 cgtcataact gaacgcgaat gtgctgttaa ctgtaatgcc gccaagtgta tcaacaacgt
      541 ctctaaagct ttccatgttc actttcacaa aataatcaac cggaacatcg aggaagttct
      601 caacagtgtc taccgtcatc tgtgtgccgc cgaaagcgta tgaatggttg attttatcca
      661 ttgtgccttt tccgataatt tttgtatacg tgtcacgcgg aatgctgacc atgtcggttg
      721 tattcgtttt tgggttaacg gtcatataaa tgagcgtatc ggcacggccc ttatcgccgt
      781 cgcgttcatc cacacccatg attaagacgg aaaaaggatc ttttttattg atactgacct
      841 ctttatccct ctttttagat ttatcaatgc tttcatgaat acttgccacg gttgaagctg
      901 ctttatgcca taagtagtat gcatagccgc cagttccgag tacaagaagt ccgattatcg
      961 ttaaaattgt cagtaataaa gtttttttct tttttctgcg ttcgtttctc atcctttgca
     1021 cctcgtctgt taaattactt tcattatgag ttaaatttcc aataaataca actctattta
     1081 atacaagaat gaaatcctaa aatagtcgtt ttttataaaa aaaagtttca ttgtttctta
     1141 atttttcttt aaaatataaa ataggttgac gataaaatat aatgaggtga aaaaaatgaa
     1201 aaaatttatt gctttactgt tctttatatt gcttctttcg ggttgcgggg ttaatagtca
     1261 aaagagtcaa ggtgaagatg tatcgccaga cagtaacatt gaaacaaaag aaggtactta
     1321 tgtagggtta gctgatactc atacaataga agtaacagta gataatgagc cggttagtct
     1381 tgatatcact gaagaatcga caagtgatct tgataagttt aacagtggag ataaggtcac
     1441 gattacatat gaaaaaaatg atgagggtca gcttctgtta aaagatattg aacgtgccaa
     1501 ctaagatata gggaggaacc tgaaattttg aaatcttgca aacaattgat agtgtgttca
     1561 cttgctgcaa tcttattatt gattccatca gtttcttttg cagcagactc aaacatctca
     1621 gttaaattgt taaattatat tggaaataaa tctagcatca gtctatcgcc aacaggattt
     1681 tataaagtaa ctggagataa tgttgctgtt acggatcgtt ttgcaggcgc ttcacgatac
     1741 gaaactgcga ctcttgcatc caacagccag tggaaaaatc ctaatacagt gatattggtg
     1801 aaccgagata tttttattga tgcattgcct gttattccat tggcgaaaaa actgaacgca
     1861 ccagttttat ttactcagcc tgacacgtta acaaaaacta ctgaaagaca aattgctaag
     1921 tttaaccctg ataatatttt aatcattggg ggagcgcgaa gcatttcaaa agacgtagaa
     1981 aacaagctga aatcatatgg cgctgtgaaa aggataagcg gaaaaaatag atatgtctta
     2041 tcagaaaata ttgcaaagca aatgggcagc tatgataaag cgatcgttgt aaccggaaga
     2101 gtatttcaag acgctttagc cattgctcca tatgcagcag cacacggata ccctattttg
     2161 cttacagaga aagacaagct tccggattat gatttgccaa aacaagtcat cattataggc
     2221 agttcattta gcgtcagtga tagtgtagaa aaccaaatta aaaaaacttc aactgtgcaa
     2281 aggattcctg gatcaaccag atatgaactt acggcaaata taattaagca actaaagttg
     2341 aaggctgata aagtcgtaat gacgaacggg acaaaatatg cagacgtatt gataggcgct
     2401 tcgcttgcct cgaagaaaaa cagccaaatt ttatttgtta aacaagatag cgttccggct
     2461 gcggctaaaa gtataacgaa agataaagcc acatatgcat acgactttat tggaagtacc
     2521 agttcaattt ctgctgaagt tgagaattca cttgctgatg aattttattt agctgacggt
     2581 ggcacctata atctaaaaat caacagcgga aaattaaacc ttgaaaacat caaaacttac
     2641 ggaaactccc taagaattaa accagaaaac tattctactt caaacagaat tagtcttgat
     2701 ggaaagcagt atcttggcac ggttaatttc tctattgagt ctactaaata tatccgaccg
     2761 gtaaatgaga atattccatt tgaggattac ttaaaaggag taattccgaa tgaaatgcct
     2821 gctagctggt cacttgaggc tcttaaagca cagactgtcg ctgcaagaac ctactcaata
     2881 acaaaaacag gaactactgt acctgataca accgcttttc aagtttatgg tggctacagt
     2941 tggaattcga ataccaataa agcggtagag caaacgaagg ggaaagttct gaaatataat
     3001 ggatctctta taactgcagc ttattcttcc agtaatggtg gttacacaga agctagtaat
     3061 gaagtgtggt cgtcaagtgt accttacttg attgcaaaaa aagatacaaa ggatcctcaa
     3121 ataggctgga cgcttacttt gtcaaaacaa cagctagata cgaaatcttt ggacttaacg
     3181 aagccttctt cttggtggtc ttcagcgact gaaactgatt ctgcaagact ttccggagtt
     3241 aagaactgga tcttgaaaaa taaagaaaca agcgctgatt ctgtcaaaat tgcaagtatt
     3301 gacgatttaa gctttagtgg aacgacacaa gggcagcgtg caaaaacagc atcgatgaaa
     3361 gtgaagtatt ttgttaaaag cagtactggc tcctataact tgagcaaaat tacaaccatt
     3421 agtgtaccaa caagtgagtt aagaacaatg attggagcga ctgtatttaa aagtacttat
     3481 gtgactgtaa aaaaagatac ttcaaagtac acaatcagcg gtaaaggata tggtcacggg
     3541 atcggaatga gtcaatatgg agcaaaagca agagcagaag ctggagattc atatagttca
     3601 attttgaaat tttactatcc aggtacaact ctgacaagtt attgattatt ttaggatata
     3661 acgccattta aggaggaaat caattgcgtt cttatataaa agtcctaaca atgtgttttc
     3721 tggggctcat actttttgtg ccaacagctt tggccgataa ctcagtgaaa agagttgggg
     3781 gaagcaatag atacggcact gctgtacaaa tatcaaagca aatgtattca acagcaagta
     3841 cagctgtaat tgttggtggg agttcctatg cagatgctat ttcagcagca cctcttgctt
     3901 accagaagaa tgcgccattg ctttacacta attctgataa gctttcatat gaaacgaaaa
     3961 caagattgaa agagatgcag actaaaaatg taattattgt aggcggaaca cctgctgttt
     4021 cttctaacac tgctaaccag attaaaagct tggggataag tattaaacga attgcaggaa
     4081 gcaaccgtta tgatacggct gcacgggtgg caaaagcgat gggtgcgact tcaaaagctg
     4141 ttattttgaa cggcttctta tatgcagacg ctccggccgt catcccttat gcagcgaaaa
     4201 acgggtatcc aattcttttt acaaataaaa catctataaa tagtgcgact acgtctgtga
     4261 taaaagataa gggaatttcg agtaccgttg ttgtaggagg cactggaagt atcagcaata
     4321 cggtatacaa caagttacct tctcctacaa gaattagcgg ttcaaacaga tatgagcttg
     4381 ctgcaaatat cgtacaaaaa cttaatttat caacaagcac cgtatatgta agcaatggat
     4441 tcagctaccc tgactctatt gcaggagcta cactggcagc taagaagaag caatctctta
     4501 ttcttacaaa tggtgaaaat ttatctacag gagcccgtaa aattattgga agtaaaaaca
     4561 tgtcaaactt tatgattatc ggaaacactc ctgccgtaag cacaaaggtt gctaatcagc
     4621 taaagaatcc agttgtaggt gaaacaatct ttattgatcc gggtcacggt gatcaagatt
     4681 caggagcaat cggcaatgga ctccttgaga aagaagtcaa ccttgatata gcgaaaagag
     4741 tcaatacaaa gctaaatgct tcaggtgctc ttccagtact gtcaagatct aatgatactt
     4801 tttattcttt acaggagaga gtaaataaag cagcttctgc acaagcagat ttatttctca
     4861 gtatacatgc aaatgctaat gatagctcat caccaaatgg aagtgagacg tactacgata
     4921 caacatatca agctgcaaat agcaagagac tggctgaaca aattcaacca aagttagcgg
     4981 ctaatcttgg aacgagagac cggggagtaa aaacagctgc tttctatgtt attaaatatt
     5041 ctaaaatgcc gagtgtttta gttgaaactg cctttatcac taatgcatca gatgcaagta
     5101 aattgaagca agcggtttat aaagataaag ctgcacaagc tattcatgac ggcacagtat
     5161 cttattacag ataatcgaaa gagacaaatc taatcacaga tttgtctctt ttttatatga
     5221 aatgattaaa acaaatagga cttgtatata aaatagtaaa agagtattaa caacatttat
//