From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Pseudomonas sp. nitrite reductase (nirA) gene, complete cds.
Date: 24 Jul 92 10:09:09 GMT
Distribution: bionet
Checksum: 24949     6

LOCUS       PSENIRAX     1886 bp ds-DNA             BCT       24-JUL-1992
DEFINITION  Pseudomonas sp. nitrite reductase (nirA) gene, complete cds.
ACCESSION   M97294
KEYWORDS    nirA gene; nitrite reductase.
SOURCE      Pseudomonas aeruginosa (individual_isolate G-179, strain sp) DNA.
  ORGANISM  Pseudomonas aeruginosa
            Prokaryotae; Gracilicutes; Scotobacteria; Pseudomonadaceae.
REFERENCE   1  (bases 1 to 1886)
  AUTHORS   Hoitink,G.C., Woudt,L.P., Tourenhout,J.C., Va De Kamp,M. and
            Canters,G.W.
  TITLE     Isolation and sequencing of the Alcaligenes denitrificans
            azurin-encoding gene:comparison with the genes encoding blue copper
            proteins from Pseudomonas aeruginosa and Alcaligenes faecalis
  JOURNAL   Gene 90, 15-20 (1990)
  STANDARD  full staff_review
REFERENCE   2  (bases 1 to 1886)
  AUTHORS   Galimand,M., Gamper,M., Zimmermann,A. and Haas,D.
  TITLE     Positive FNR-like control of anaerobic arginine degradation and
            nitrate respiration in Pseudomonas aeruginosa
  JOURNAL   J. Bacteriol. 173, 1598-1606 (1991)
  STANDARD  full staff_review
REFERENCE   3  (bases 1 to 1886)
  AUTHORS   Ye,R.W., Fries,M.R., Bezborodnikov,S.G., Averill,B.A. and
            Tiedje,J.M.
  TITLE     A copper nitrite reductase gene and its homology to other
            denitrifiers
  JOURNAL   Theses-Michigan State University (1992) In press
  STANDARD  full staff_review
FEATURES             Location/Qualifiers
     protein_bind    289..303
                     /function="activates transcription"
                     /bound_moiety="FNR protein"
                     /standard_name="FNR box"
                     /gene="nirA"
                     /note="putative"
                     /citation=[2]
                     /citation=[3]
                     /citation=[1]
     promoter        580..595
                     /function="facilitates recognition of RNA polymerase to
                     the promoter"
                     /standard_name="NtrA box (sigma-54 binding site)"
                     /gene="nirA"
                     /citation=[3]
                     /citation=[1]
     promoter        623..639
                     /function="facilitates recognition of RNA polymerase to
                     the promoter"
                     /standard_name="NtrA box (sigma-54 binding site)"
                     /gene="nirA"
                     /citation=[3]
                     /citation=[1]
     RBS             654..660
                     /gene="nirA"
     sig_peptide     668..763
                     /gene="nirA"
                     /codon_start=1
     CDS             668..1807
                     /product="nitrite reductase"
                     /gene="nirA"
                     /codon_start=1
                     /translation="MSEQFRLTRRSMLAGAAVAGALAPVVTSVAHAEGGGIKTNSAAT
                     AANIATLERVKVELVKPPFVHAHTQKAEGEPKVVEFKMTIQEKKIVVDDKGTEVHAMT
                     FDGSVPGPMMIVHQDDYVELTLVNPDTNELQHNIDFHSATGALGGGALTVVNPGDTAV
                     LRFKATKAGVFVYHCAPAGMVPWHVTSGMNGAIMVLPRDGLKDHKGHELVYDKVYYVG
                     EQDFYVPKDENGKFKKYESAGEAYPDVLEAMKTLTPTHVVFNGAVGALTGDNALQAKV
                     GDRVLILHSQANRDTRPHLIGGHGDYVWATGKFANPPELDQETWFIPGGAAGAAYYTF
                     QQPGIYAYVNHNLIEAFELGAAGHFKVTGDWNDDLMTAVVSPTSG"
     mat_peptide     764..1804
                     /function="nitrite reduction"
                     /standard_name="Cu-NiR"
                     /product="nitrite reductase"
                     /gene="nirA"
                     /note="putative"
                     /codon_start=1
                     /citation=[3]
BASE COUNT      439 a    501 c    554 g    392 t
ORIGIN
        1 aattcggccg tgccggggta atgtcgtgag ggccgggtgg gccgtagacg atttgatagg
       61 cccaaactgc gaaaccgtag ctggcgacgg taccaacggc cacgactggc caaatgccaa
      121 aagcgagaac gacaaaggtc agcagctcgc ggcgccgctt ttggcggaag gatgtctgtt
      181 cgagagttgc tatcggctct gccatgttca cttccggtct tcagcaaaat ctgaaatgca
      241 aaaatgcact aaatgcctca aatataacca aatccgtcca tcactcgcct tgatgaaaat
      301 caaatttagt cgcggttcgc tgaaaaaata tgcgtaaaga ttaaaaagca gacacacata
      361 agttgtgcca tgagcgacgc acaacattaa ggacaaagct gctcatcact caaatagata
      421 ccaataatcg tttttttgct cagctgctgt actcgcctcc gatgagcaca acttcctttc
      481 cctaacgctt gagtgttccc tcaaaaacag cattaatcaa gtggccgtgt gtatgttcgt
      541 gtaatctacc tataggcgaa aactctcgtt ttatttgtgt tggagcaaac atgctcgcga
      601 atgtcgatct acgttcaggg ctgtggagcc gaggttgctc cgcttcttga aaaagggaga
      661 gactttcatg agtgaacagt ttcggttgac ccgccggagc atgctggccg gcgcagctgt
      721 cgcgggagca ctagcgccgg tcgtcacctc tgttgcacat gccgaaggcg ggggtatcaa
      781 gacaaattct gctgcgacag cagcaaacat cgcgacgctg gagcgcgtca aggtggaact
      841 ggtcaagcca ccctttgtgc acgcccacac ccagaaggct gagggcgagc ccaaggttgt
      901 cgagttcaag atgaccatcc aggaaaagaa gatcgttgtc gacgacaagg ggaccgaggt
      961 ccatgcgatg acgttcgacg gatctgtgcc ggggccgatg atgatcgttc accaggatga
     1021 ttatgtcgaa ctgaccctcg tcaatcccga tacaaacgaa ttgcagcaca atatcgactt
     1081 ccattcggcg acgggtgcgc tgggtggtgg agcgctgacc gtggtcaatc ccggtgacac
     1141 ggcggtgctg cgtttcaagg ccaccaaggc gggtgttttt gtctatcact gtgcccccgc
     1201 cggcatggtg ccgtggcatg tcacttcggg catgaatggt gcgatcatgg tcctgccgcg
     1261 tgacggtctc aaggatcaca agggccacga gcttgtttac gacaaggtct actatgtcgg
     1321 cgagcaggac ttttatgtcc cgaaggatga gaacggcaag ttcaagaaat acgaaagcgc
     1381 cggcgaggcc tatccggatg tgctggaagc catgaagaca ctgaccccga cgcatgtcgt
     1441 gttcaacggg gcggtcggcg ccctgacggg cgataacgcc ttgcaggcaa aggtgggcga
     1501 tcgtgtcctg attcttcatt cgcaagccaa cagggatacg cgcccgcacc tgatcggggg
     1561 gcatggcgat tatgtctggg ctaccggcaa gttcgccaac ccgccggaac tcgatcagga
     1621 aacctggttc attcccggag gtgctgccgg ggcggcttac tacacgttcc agcagcccgg
     1681 tatctatgcg tatgtaaacc acaatctgat cgaggcgttc gaactcggcg cggccggcca
     1741 cttcaaggtg acgggcgact ggaacgacga cctgatgaca gccgtggttt cgccgacctc
     1801 gggttgacgg tgtcggcccg gcgccggatc aggcgccggg ccacatcctt atgccggccg
     1861 ggggaggccg gaaggatcgg aggatc
//