From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Saccharomyces cerevisiae (POB1) gene, complete cds.
Date: 26 Jan 93 05:53:51 GMT
Distribution: bionet
Checksum: 42092 8

LOCUS       YSCPOB1A     3718 bp ds-DNA             PLN       25-JAN-1993
DEFINITION  Saccharomyces cerevisiae (POB1) gene, complete cds.
ACCESSION   M94769
KEYWORDS    .
SOURCE      Saccharomyces cerevisiae DNA.
  ORGANISM  Saccharomyces cerevisiae
            Eukaryota; Fungi; Eumycota; Hemiascomycetes; Endomycetales;
            Saccharomycetaceae.
REFERENCE   1  (bases 1 to 3718)
  AUTHORS   Miles,J. and Formosa,T.
  TITLE     Evidence that POB1, a Saccharomyces cerevisiae protein that binds
            to DNA polymerase alpha, acts in DNA metabolism in vivo
  JOURNAL   Mol. Cell. Biol. 12, 5724-5735 (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     CDS             321..3104
                     /function="apparent DNA replication accessory factor"
                     /gene="POB1"
                     /evidence="EXPERIMENTAL"
                     /citation=[1]
                     /note="initiating methionine is not found in the mature
                     protein;  ;the N-terminal sequence of the protein is
                     VSVID.."
                     /codon_start=1
                     /translation="MVSVIDKLVFDFGGKTLVSLAPDNNTLCVANKNGLTKILKTNNP
                     EEEPETLDSSKLVSSIKCYSNSHFLMTTMQGDALRYNIDSSQEELLARFALPLRDCCV
                     IHSGKMAVFGGDDLELILLELDDETHKKHAIKIDEQVSQISYNSQMNILAVSMINGKV
                     QIFSLTSTIPNKVHELNDYIVANSYDDTHRDKILSNMMDDIDKDNDNDLSETADPDEN
                     NVADPEFCAANRICTRVAWHPKGLHFALPCADDTVKIFSIKGYSLQKTLSTNLSSTKA
                     HFIDLQFDPLRGTYIAAVDLNNKLTVWNWETSEIHYTREFKRKITNIAWKIQADSKTL
                     DLVLGTWSGSIAIVQNLAESVVSNIPDQSVAESSTKHGLFVDSESDLENLEGNDDINK
                     SDKLFSDITQEANAEDVFTQTHDGPSGLSEKRKYNFEDEEDFIDDDDGAGYISGKKPH
                     NEHSYSRVHKTHSFPISLANTGKFRYMPFSPAGTPFGFTDRRYLTMNEVGYVSTVKNS
                     EQYSITVSFFDVGRFREYHFEDLFGYDLCFLNEKGTLFGQSKTGQIQYRPHDSIHSNW
                     TKIIPLQAGERITSVAATPVRVIVGTSLGYFRSFNQFGVPFAVEKTSPIVALTAQNYR
                     VFSVHYSQFHGLSYSLSELGTSSKRYYKRECPLPMSLPNINSDMKKDANLDYYNFNPM
                     GIKSLFFSSYGDPCIFGSDNTLLLLSKWRSPEESKWLPILDSNMEIWKMSGGKETTDI
                     HVWPLALAYDTLNCILVKGKHIWPEFPLPLPSEMEIRMPVFVKSKLLEENKAILNKKN
                     EIGADTEAEEGEEDKEIQIPVSMAAEEEYLRSKVLSELLTDTLENDGEMYGNENEVLA
                     ALNGAYDKALLRLFASACSDQNVEKALSLAHELKQDRALTAAVKISERAELPSLVKKI
                     NNIREARYEQQLK"
BASE COUNT     1288 a    639 c    742 g   1049 t
ORIGIN      
        1 acaaggctta tccatattcg tatatatctt cataatgttt cataattgct tcagaaaatt
       61 attcaaaacg tcttgtgata ttcattaggt gaaatcgtag tttgcctgat acttgaaaga
      121 atctcacctg gagaatgggt gttttcacta agattcgtta atttccacta atgattaatt
      181 gttaactgtt taacgcgacg cgtaataaag tttcctgaat acgccaacat atgggaacat
      241 atagattaaa ttaataagaa agcttgggaa aaataattga gaagggcaag aagtgacgta
      301 aatatactag acgtactatt atggtttcag ttatagacaa gcttgttttt gactttggtg
      361 ggaaaactct ggtctctctc gcaccagata ataatacttt gtgtgtagcc aataaaaatg
      421 gcttaaccaa gattctgaag acaaataacc cagaagaaga gccagagact ttagattctt
      481 ccaaattggt ctcgtctata aaatgctatt caaactcgca ctttctgatg actacaatgc
      541 aaggtgatgc tcttaggtat aacatcgatt ctagtcaaga agaattattg gctagatttg
      601 ctttacccct acgtgactgc tgtgttattc attcaggtaa aatggccgta tttggaggag
      661 acgacttaga actaatcctt ttggaattgg acgatgaaac acacaaaaaa catgccatta
      721 aaatcgatga acaagtttct caaatttctt acaattcaca gatgaatatt ttagcagttt
      781 caatgataaa tggtaaggta caaatttttt ctctgacatc tactattcca aacaaagttc
      841 atgagttaaa tgattacata gtggccaatt catatgatga tacacacaga gataagatac
      901 tctcgaatat gatggatgat atagataaag acaatgataa tgacctgagt gaaacggctg
      961 atccagatga gaacaatgta gctgatccag aattctgtgc tgctaataga atttgcacga
     1021 gagtggcttg gcatccgaag ggtctgcatt ttgcgctacc atgtgcagat gatacagtaa
     1081 aaatattctc tataaaggga tattccctac aaaagacgtt gtccacaaat ctctcatcaa
     1141 caaaggctca tttcattgat ttgcaatttg acccgttacg tggaacttac attgcggcag
     1201 tagatttaaa taataagtta acggtatgga attgggaaac ttccgagatc cactacacca
     1261 gggaattcaa aagaaaaatt actaatattg cctggaaaat ccaagcggac tcaaaaactc
     1321 tagaccttgt tttaggtacc tggtccggta gtatagccat tgtccaaaat ttggcagagt
     1381 ccgtagtatc taatatacct gaccaatctg ttgctgaatc ttcaactaaa catgggcttt
     1441 ttgtagactc cgaatctgac ttggaaaact tagagggaaa tgatgatata aacaaaagcg
     1501 ataagctatt ttcagatatt actcaagaag cgaatgcgga agatgtgttc actcaaacac
     1561 acgacggccc cagtggatta agtgaaaaga gaaaatacaa cttcgaagat gaagaagact
     1621 ttattgatga tgacgatggt gctggttata ttagtggcaa aaaaccacat aatgaacatt
     1681 cttattcgag agtacacaag actcattcgt ttccaatcag tttggcaaac acaggaaaat
     1741 ttcgttatat gcctttttct ccagcgggaa caccttttgg ctttactgac aggcgttatt
     1801 tgacaatgaa tgaagtgggc tacgtatcta ctgtcaagaa tagtgagcaa tacagcataa
     1861 ctgtctcttt ttttgatgtt ggacgtttta gagaatacca tttcgaggac ttatttggct
     1921 acgatttatg cttcctaaat gaaaaaggca ctttatttgg ccaatccaaa actgggcaga
     1981 tacaatatag gccacacgat agcatacatt caaactggac caagattatt cctttgcaag
     2041 ctggtgagag aataacaagt gtggcagcca ccccggttcg cgttattgtt ggtacatcat
     2101 taggctattt cagaagtttc aatcaattcg gggttccatt tgctgttgaa aagacatccc
     2161 caattgtagc gcttactgct cagaattata gggttttttc agtacattat tcgcagtttc
     2221 atggcctttc atactcttta tctgaattgg gtacttctag taaaaggtac tataaaagag
     2281 agtgtccact tccaatgagt ttaccaaaca ttaattctga tatgaaaaaa gacgcaaatc
     2341 ttgactacta caattttaat ccgatgggca tcaaaagttt gttcttttca agctacggag
     2401 atccatgcat ttttgggtcc gacaacacgc ttctattgtt atcaaagtgg agatcaccag
     2461 aagaaagtaa atggctccct attctagata gcaacatgga aatatggaag atgtcaggag
     2521 ggaaggaaac gacagatata catgtctggc ccttggcttt ggcgtatgac acattgaatt
     2581 gtatcttagt taagggcaag catatatggc ccgagtttcc ccttccgttg ccatccgaaa
     2641 tggagattag aatgccagta tttgttaaga gtaaattact agaggaaaac aaagctatat
     2701 taaataaaaa gaacgaaatt ggagctgaca ccgaagcgga agaaggggaa gaagacaaag
     2761 aaatacagat tcctgtttct atggcggcgg aagaagagta tctgcgcagc aaggttttgt
     2821 cagagctgtt gacagataca ctcgaaaatg acggtgaaat gtacggcaac gaaaatgagg
     2881 tattggcagc attgaacggt gcatatgata aggctttgtt acgtttattt gcgtctgcat
     2941 gctcagacca aaatgttgaa aaggctcttt cgcttgctca tgaattaaaa caagatagag
     3001 cacttactgc agccgttaag atatcagaaa gagctgagct gccctctctc gttaaaaaaa
     3061 ttaataatat aagggaagct agatatgaac agcaattgaa ataaaaaaat gtaaaatata
     3121 tatacgcaag agacaattat ttgatacctg ttcatttgtt caaatcatcg ttcaaaatgc
     3181 tcccacatta acatatattt ctttgaatgc gacgataata aagagttacg ttgaacatga
     3241 tctgagctgg aagcgtcctt gcgaattttc gcgaaaggaa aaagtgaaaa aatatgaaaa
     3301 aaaaaaaaag agatgacatt ttatagatga gctttgatta aacagtgtaa cacctggtcc
     3361 tgtaccgtaa atattcaaat aagcatcaat tcaaagttct ttatttcttc tttagcattt
     3421 caaaattata gaaaatctag aaaagatgtc agatgttacc caacagaaaa agaggaaaag
     3481 atccaaagga gaagttaatc catcaaaacc cacagtagac gaagaaatca cagatccatc
     3541 ctcaaacgaa gatgaacaac tagaagtttc ggatgaagaa gacgcattgg aatctgaaga
     3601 agaattcgag ggtgaaaatc cagccgataa aagaaggagg cttgcaaagc aatatctgga
     3661 gaatctgaaa agcgaacgta atgacatttt aactgataat aggaacgctg aagaaaaa
//