From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Plectonema boryanum gene for dinitrogenase-like protein.
Date: 12 Jan 93 05:49:25 GMT
Distribution: bionet
Checksum: 57631 6

LOCUS       PEEDLP       1960 bp ds-DNA             BCT       12-JAN-1993
DEFINITION  Plectonema boryanum gene for dinitrogenase-like protein.
ACCESSION   D12973
KEYWORDS    ORF467; chlN; dinitrogenase-like protein;
            light-independent reduction of protochlorophyllide.
SOURCE      Plectonema boryanum (strain IAM-M101) DNA.
  ORGANISM  Plectonema boryanum
            Prokaryotae; Bacteria; Gracilicutes; Oxyphotobacteria;
            Cyanobacteria; Nostocales; Scytonemataceae.
REFERENCE   1  (sites)
  AUTHORS   Fujita,Y., Takahashi,Y., Shonai,F., Ogura,Y. and Matsubara,H.
  TITLE     Cloning, nucleotide sequences and differential expression of the
            nifH and nifH-like (frxC) genes from the filamentous
            nitrogen-fixing cyanobacterium Plectonema boryanum
  JOURNAL   Plant Cell Physiol. 32, 1093-1106 (1991)
  STANDARD  full automatic
REFERENCE   2  (bases 1 to 1960)
  AUTHORS   Fujita,Y., Matsumoto,H., Takahashi,Y. and Matsubara,H.
  TITLE     Identification of the nifDK-like gene (ORF467) involved in the
            biosynthesis of chlorophyll in the cyanobacterium Plectonema
            boryanum
  JOURNAL   Plant Cell Physiol. (1993) In press
  STANDARD  full automatic
COMMENT     Submitted (20-AUG-1992) to DDBJ by: Yuichi Fujita Institute for
            Protein Research Osaka University 3-2 Yamadaoka, Suita Osaka 565
            Japan Phone:  06-877-5111 x3852 Fax:    06-876-2533.
FEATURES             Location/Qualifiers
     RBS             58..60
     repeat_region   1505..1537
                     /rpt_type=inverted
     CDS             1..44
                     /partial
                     /product="ORF133"
                     /codon_start=3
                     /translation="ACEGRNRTTPSPQ"
     CDS             71..1474
                     /note="homologous to Marchantia polymorpha chloroplast
                     ORF465, to Rhodobacter capsulatus bchN gene and to
                     Chlamydomonas reinhardtii chloroplast chiN gene"
                     /product="ORF467"
                     /codon_start=1
                     /translation="MTLADAQPQALNFECETGNYHTFCPISCVAWLYQKIEDSFFLVI
                     GTKTCGYFLQNAMGVMIFAEPRYAMAELEEGDISAQLNDYNELKRLCEQIKRDRNPSV
                     IVFIGTCTTEIIKMDLEGLAPKLESEIGIPIVVARANGLDYAFTQGEDTVLAAMAQRC
                     PTQAPTAEADKEERNAIQKLMNFGRKQEDVKREESEYVDHPPLVMFGSVPDPIVTQLS
                     LELKHQGIKVSGWLPAKRYTELPVIEEGYYVSGVNPFLSRTATTLMRRRKAKLIGSPF
                     PIGPDGTRAWVEKICSVFNIEPKGLEEREAKIWQSVEDYLQLIRGKSVFFMGDNLLEI
                     SLARFLIRCGMTCHEIGIPYMDKRYQAAELDFLVKTCQEMGVPVPTIVEKPDNYNQLQ
                     RIHELKPDLVITGMAHANPLEARGISTKWSVEFTFAQIHGFGNTRDILELVTRPLRRN
                     GALKDLGWEKLVEEARV"
     CDS             complement(1548..1958)
                     /partial
                     /product="URF2"
                     /codon_start=1
                     /translation="IWDKRFKQINHFEQYLVENGIVVLKFFLNVSKEQQKKRFLARIN
                     EPEKNWKFSVSDAKERQHWDQYMEAFEDVFNNTSTSYAPWYIIPADHKWFTRLAVSDI
                     IVSTLKSLHLSYPTVSEVRRAELLEAKKVLESEE"
BASE COUNT      545 a    408 c    483 g    524 t
ORIGIN      
        1 aagcttgcga aggacgcaac cgaacgacac catcgcctca ataaaatcct cactttagag
       61 acctactgtt atgactctcg ctgatgctca acctcaagcc ctaaacttcg agtgcgaaac
      121 tgggaactat cacacttttt gtccgattag ctgtgttgcc tggctatatc aaaaaattga
      181 agatagcttt ttcctcgtta ttggcacgaa aacttgcggc tattttctgc aaaacgcgat
      241 gggcgtgatg atttttgcag aacctcgcta tgccatggca gaactggaag aaggcgatat
      301 ttctgctcag ttgaacgatt acaacgaatt gaagcggctc tgtgagcaga ttaagcgcga
      361 tcgtaatccc agtgtgattg tgttcatcgg tacttgcacc accgaaatta tcaaaatgga
      421 tttagaaggg ctagcaccga aactcgaatc cgagattggt attcccattg tcgttgcgcg
      481 ggcaaatggt ctagattacg cctttacaca aggagaagac acggttttag cagcaatggc
      541 gcaacgctgt ccaacccaag ctccaaccgc ggaagcagac aaagaggagc gtaatgcaat
      601 ccagaagttg atgaactttg gacgcaaaca ggaagatgtg aagcgcgaag agtcagaata
      661 tgtggatcat ccgccgctcg taatgtttgg gtctgttcct gatccgatcg tgactcagct
      721 ttccttggaa ttaaagcatc aagggatcaa ggtttcagga tggttgcctg cgaagcgtta
      781 tactgagctt cccgtaatcg aggaaggata ctatgtgtcg ggcgtgaatc cgttcctgtc
      841 aagaactgct acaacattga tgcgacgacg gaaagcgaaa ttgattggat cgccctttcc
      901 aattgggcca gatggaacaa gagcttgggt tgaaaagatt tgctcagtgt ttaacattga
      961 gccgaaagga ttagaggagc gggaagcaaa aatttggcaa agtgttgaag actacctgca
     1021 attgattcgc ggcaagtcag tgttcttcat gggcgataac ttgctcgaga tttcgctggc
     1081 tcggtttttg attcggtgcg ggatgacttg tcatgagatc ggcattccgt acatggacaa
     1141 gcggtatcag gcagcggaat tagatttctt agtgaaaacc tgtcaagaaa tgggcgttcc
     1201 agttccgacg atcgttgaaa agccagataa ctataatcag ttgcagcgaa ttcatgaatt
     1261 gaaaccggac ttagtcatta cgggaatggc tcacgcaaat ccactagagg cgcgtggaat
     1321 tagtacgaag tggtcggtcg agtttacgtt tgctcagatt cacggatttg ggaatacgcg
     1381 ggacattttg gagttagtaa cacgaccact gcggcgaaac ggggcgttga aggatttagg
     1441 ttgggaaaaa ctggtagagg aagcgcgagt ttaaagcgtt actcactcta acctacgaaa
     1501 ctttagaagg cggagttttg ggacaactcc gccttctgtt atttcaatta ttcctcgctt
     1561 tctaggactt tctttgcttc tagtaactct gcacgccgga cttcgctaac cgtgggatag
     1621 gacagatgca aagactttaa cgtactcaca ataatgtctg agacggctaa ccgggtaaac
     1681 catttatgat cggcggggat aatataccaa ggagcatagc tagtactcgt attgttaaat
     1741 acatcttcaa aagcttccat gtattgatcc cagtgttgcc gttctttagc atcagagaca
     1801 gagaacttcc aatttttttc gggttcatta atccgagcta gaaatcgttt cttttgttgt
     1861 tccttagaaa cattcaaaaa gaacttgagt acaacaatgc cattttcgac aagatactgt
     1921 tcaaaatggt tgatctgctt gaatcgtttg tcccagatct
//