From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Pseudanabaena sp. phycocyanin-2 beta subunit (cpcB2); phycocyanin-2
Date: 20 Aug 92 10:04:26 GMT
Distribution: bionet
Checksum: 49913     7

LOCUS       PUNCPCBII    2757 bp ds-DNA             BCT       20-AUG-1992
DEFINITION  Pseudanabaena sp. phycocyanin-2 beta subunit (cpcB2); phycocyanin-2
            alpha subunit (cpcA2); and phycocyanin-associated peripheral
            rod-linker protein (cpcH2) genes, complete cds's.
ACCESSION   M99427
KEYWORDS    phycocyanin-2 alpha subunit; phycocyanin-2 beta subunit;
            phycocyanin-associated peripheral rod-linker protein;
            rod-linker protein.
SOURCE      Pseudanabaena sp. (library: PCC 7409) DNA.
  ORGANISM  Pseudanabaena sp.
            Prokaryotae; Gracilicutes; Oxyphotobacteria; Cyanobacteria;
            Nostocales; Oscillatoriaceae.
REFERENCE   1  (bases 1 to 2757)
  AUTHORS   Dubbs,J.M. and Bryant,D.A.
  TITLE     Organization and transcription of the genes encoding two
            differentially expressed phycocyanins in the cyanobacterium
            Pseudanabaena sp. PCC 7409
  JOURNAL   Unpublished (1992)
  STANDARD  full automatic
FEATURES             Location/Qualifiers
     RBS             7..11
     CDS             19..471
                     /standard_name="ORFX"
                     /note="putative"
                     /codon_start=1
                     /translation="MTGDLLTSERGLFDLQLELKELDQPTTYQMLINYLNSVRIKNDC
                     KDINDPRSVIPFLPETAKKFCEVSLGKPRLFNRLGSTVLLTATGGSGRSHYSRCIRKR
                     VESCCTKFAPSSCIECSRRESTSFITETRYLFRRNHYGRSATVRSSHL"
     RBS             1181..1186
     CDS             1196..1714
                     /function="light-harvesting protein in peripheral rods of
                     phycobilisome"
                     /product="phycocyanin-2 beta subunit"
                     /gene="cpcB2"
                     /codon_start=1
                     /translation="MLDAFTKVVAQADTRGRFVSDSQIDALKQLVADGTKRLDVVNRI
                     TASSSSIVTDAARALFEDQPQLIAPGGNAYTNRRIAACMRDMDIILRYVTYAVFSGDA
                     SVLEDRCLNGLRETYSALGVPGGSVAVGIDKMKAAAIALANDPNGVTRGDCSALMSEL
                     ASYFDKAAAAVG"
     RBS             1800..1804
     CDS             1815..2303
                     /function="light-harvesting protein found in peripheral
                     rods of phycobilisomes"
                     /product="phycocyanin-2 alpha subunit"
                     /gene="cpcA2"
                     /codon_start=1
                     /translation="MKTPLTEAVSTADSQGRYLSVPEMQAAFGRLRTATASLDAAKGL
                     GAKASSLAEGAANAVYQKYPYTTQQSGNNFASTPRGKAKCVRDIGYYVRMITYCLIAG
                     STGPMDDYLVSGLAEINRSFDLSPSWYVEALKYIKANHGLSGDAALEANSYIDYAINA
                     LS"
     stem_loop       2310..2362
     stem_loop       2385..2427
     RBS             2529..2534
     CDS             2542..2757
                     /function="assembly of phycocyanin in phycobilisome
                     peripheral rods"
                     /product="phycocyanin-associated peripheral rod-linker
                     protein"
                     /gene="cpcH2"
                     /codon_start=1
                     /translation="MTSSAAAIRLGFEPFVNASPVELRTNWSDSDVQAVISATYRQVF
                     GNEHLMLSERLTSAESLLASGNISVREF"
                     /partial
BASE COUNT      834 a    586 c    535 g    802 t
ORIGIN
        1 gttaacagga gcaatccaat gacaggagat ctattaacca gtgaacgtgg attgtttgac
       61 ctacaactag aactcaaaga gctagatcaa cccacaactt atcagatgtt gatcaactat
      121 cttaatagcg tgcgaatcaa aaatgactgc aaagatatta acgatcctcg ctccgtaata
      181 ccatttttac ctgaaacagc aaagaagttt tgcgaggttt ctctcggcaa gccccgtcta
      241 ttcaaccgct taggtagtac agtcctttta actgccaccg gaggttcagg tagaagtcat
      301 tactccagat gtattagaaa aagggttgaa agctgctgca ccaagtttgc gccttcaagc
      361 tgcattgaat gttcaagaag agagagtacg agctttatta cagaaacgcg gtatcttttc
      421 cgaagaaatc actatggaag atctgcaaca gttaggagtt cgcaccttta gcgaacttct
      481 tcccatttta gaaagactag aagaagccga tcttgcccat caattaaatc aggatgatac
      541 caaagcattt gcccctatcc tattacctgc cgatgatgac gaaccagtcc caaacgatcc
      601 tgaagaataa ctaagtacca caattaacta tgaataaata ttaagcatat cccatgcaaa
      661 ctacttaaca tttattcatc tatttacaaa tacctactca agataaaaac aagaaattgt
      721 agaaaatcac cctattcttt gttagaggca atttccataa agtaaaacgg ttaaaaaaaa
      781 tggtttgtga ttaatcttta tattaagaaa taagatcaaa tacaaagaac acttaacaat
      841 tggcaactag gtataaatag ctaaaactat tgactagcaa aggttttgca atttctgtat
      901 tcattttatc taacttgata tataacactc tatgctttgc acaaaaactg cacaaattag
      961 ttgttttaat ctttttaatc ccaaagtcgc taagccaaaa tacttatcca gtttaggttt
     1021 agttccaccg gtttagctcc attaagcaag cctgatataa attggttttc gtttaatcaa
     1081 gcatgaccga ggattctatt tggattggat atctgcataa attgatatct aaaacaatag
     1141 ctaggctagg aatagttcgc tttatataca tctttaaaaa aaggagattc tttaaatgct
     1201 agatgctttt actaaagtag ttgctcaggc agatactcgc ggacgttttg taagtgattc
     1261 tcaaattgat gctttgaagc aattagttgc agatggcacc aagcgcttgg atgtcgttaa
     1321 ccgcatcacc gctagctctt catcgatcgt tacagatgca gctcgtgctt tgtttgaaga
     1381 tcagcctcaa ttgatcgctc ctggtggaaa tgcatacacc aatcgtcgta tcgccgcttg
     1441 tatgcgcgac atggacatca tccttcgcta tgtaacctac gccgtattct ctggcgatgc
     1501 tagcgttttg gaagatcgtt gcctcaatgg cttgcgtgaa acctacagcg cacttggggt
     1561 acctggtgga tcagttgcag ttggcatcga caaaatgaag gcagcagcga tcgcacttgc
     1621 taacgatcct aatggcgtaa cccgtggcga ttgcagcgct ctcatgtccg aactagctag
     1681 ctacttcgac aaagcagcag cagcagtagg ctaaatcatt aaattttttg cattaatttt
     1741 ttgcaatatt ttattttttt ctttttctcc caatttcaca acatctcata taaattccta
     1801 gaggtacatt aagcatgaag actcctttaa ccgaagcagt ttctacagca gattcccaag
     1861 gtcgctatct cagcgttcct gaaatgcaag cagcttttgg tcgcctacgc acagcaacag
     1921 ctagccttga tgctgctaaa ggattaggcg ctaaagcttc tagcttggct gaaggtgcag
     1981 caaatgctgt ttatcagaaa tatccttaca ccacccaaca atcaggcaac aacttcgctt
     2041 ctacccctcg cggtaaagcc aagtgtgttc gtgacatcgg ctactatgtt cgcatgatca
     2101 cctactgcct cattgctggt agcacaggtc ctatggatga ctatctagta agtggtttgg
     2161 cagaaatcaa tcgctctttc gatctttctc ctagctggta tgttgaagct ttgaaataca
     2221 tcaaggctaa tcatggcttg agcggagatg cagctttgga agctaattcc tacatcgact
     2281 acgcaatcaa tgccctcagc taaggttttc ttgaggaagt catgaattgc ggcgcagagc
     2341 gtcgcaattc atgacaccaa aatcatccca ataaagaaaa acccgaagac ggtaagtaca
     2401 ccctgagggg acgtgcttac cgttttctgc attgaggctg ttattgatct atattggtta
     2461 cactaatcgg cacaaaatgc cgattagata gctaagaaaa ttttaaaaaa tcattaaaaa
     2521 aatttattaa ggagcttcga tatgacaagt tctgcggcag caatccgttt aggatttgag
     2581 ccttttgtta acgcttctcc tgtggaattg cggacaaatt ggagtgattc tgacgttcaa
     2641 gcagtcattt ctgcaaccta tagacaggta tttggtaacg agcatctgat gttgtcagaa
     2701 cgcttaacca gtgctgagtc tctacttgca agtggcaata tttccgttcg tgaattc
//