From: GenBank-Updates@net.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Plasmid ColE1, complete genome.
Date: 8 Jan 93 05:46:11 GMT
Distribution: bionet
Checksum: 29314 25

LOCUS       CE1CG        6646 bp ds-DNA   Circular  BCT       07-JAN-1993
DEFINITION  Plasmid ColE1, complete genome.
ACCESSION   J01566 M33100
KEYWORDS    cea gene; colicin; colicin E1; colicin E1 immunity;
            complete genome; kil gene; origin of replication.
SOURCE      Plasmid ColE1 DNA.
  ORGANISM  Plasmid ColE1
            Prokaryotae.
REFERENCE   1  (bases 1008 to 1370)
  AUTHORS   Tomizawa,J.-i., Ohmori,H. and Bird,R.E.
  TITLE     Origin of replication of colicin E1 plasmid DNA
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 74, 1865-1869 (1977)
  STANDARD  full automatic
REFERENCE   2  (bases 1410 to 1599)
  AUTHORS   Bastia,D.
  TITLE     Determination of restriction sites and the nucleotide sequence
            surrounding the relaxation site of ColE1
  JOURNAL   J. Mol. Biol. 124, 601-639 (1978)
  STANDARD  full automatic
REFERENCE   3  (bases 623 to 832)
  AUTHORS   Morita,M. and Oka,A.
  TITLE     The structure of a transcriptional unit on colicin E1 plasmid
  JOURNAL   Eur. J. Biochem. 97, 435-443 (1979)
  STANDARD  full automatic
REFERENCE   4  (bases 1 to 1613; 6577 to 6646)
  AUTHORS   Oka,A., Nomura,N., Morita,M., Sugisaki,H., Sugimoto,K. and
            Takanami,M.
  TITLE     Nucleotide sequence of small ColE1 derivatives: Structure of the
            regions essential for autonomous replication and colicin E1
            immunity
  JOURNAL   Mol. Gen. Genet. 172, 151-159 (1979)
  STANDARD  full automatic
REFERENCE   5  (bases 1 to 192; 384 to 519; 6644 to 6646)
  AUTHORS   Patient,R.K.
  TITLE     Characterization of in vitro transcription initiation and
            termination sites in Col E1 DNA
  JOURNAL   Nucleic Acids Res. 6, 2647-2665 (1979)
  STANDARD  full automatic
REFERENCE   6  (bases 4783 to 5353)
  AUTHORS   Ebina,Y., Kishi,F., Miki,T., Kagamiyama,H., Nakazawa,T. and
            Nakazawa,A.
  TITLE     The nucleotide sequence surrounding the promoter region of colicin
            E1 gene
  JOURNAL   Gene 15, 119-126 (1981)
  STANDARD  full automatic
REFERENCE   7  (bases 639 to 753)
  AUTHORS   Tomizawa,J.-i.
  TITLE     Control of ColE1 plasmid replication: The process of binding of RNA
            I to the primer transcript
  JOURNAL   Cell 38, 861-870 (1984)
  STANDARD  full automatic
REFERENCE   8  (bases 1 to 6646)
  AUTHORS   Chan,P.T., Ohmori,H., Tomizawa,J.-i. and Lebowitz,J.
  TITLE     Nucleotide sequence and gene organization of ColE1 DNA
  JOURNAL   J. Biol. Chem. 260, 8925-8935 (1985)
  STANDARD  full automatic
REFERENCE   9  (bases 5046 to 5110)
  AUTHORS   Parker,R.C.
  TITLE     Mitomycin C-induced bidirectional transcription from the colicin E1
            promoter region in plasmid ColE1
  JOURNAL   Biochim. Biophys. Acta 868, 39-44 (1986)
  STANDARD  full automatic
REFERENCE   10  (sites)
  AUTHORS   Stirling,C.J., Szatmari,G., Stewart,G., Smith,M.C. and
            Sherratt,D.J.
  TITLE     The arginine repressor is essential for plasmid-stabilizing
            site-specific recombination at the CoIE1 cer locus
  JOURNAL   EMBO J. 7, 4389-4395 (1988)
  STANDARD  full automatic
REFERENCE   11  (bases 57 to 123)
  AUTHORS   Zhiang,S., Yan,L. and Zubay,G.
  TITLE     Regulation of gene expression in plasmid ColE1: Delayed expression
            of the kil gene
  JOURNAL   J. Bacteriol. 170, 5460-5467 (1988)
  STANDARD  full automatic
REFERENCE   12  (sites)
  AUTHORS   Inoue,N. and Uchida,H.
  TITLE     Transcription and initiation of ColE1 DNA replication in
            Escherichia coli K-12.
  JOURNAL   J. Bacteriol. 173, 1208-1214 (1991)
  STANDARD  full automatic
COMMENT     The L (light) strand is shown.  This sequence was kindly supplied
            over computer network by J.Lebowitz (04-APR-1985).  The open
            reading frames noted by [8] are named in FEATURES by the molecular
            mass of their predicted products or by the names given by [8].
FEATURES             Location/Qualifiers
     mRNA            complement(<59..409)
                     /note="imm mRNA [5],[8]"
     mRNA            complement(<3943..4887)
                     /note="exc mRNA [5],[8]"
     mRNA            join(5056..6646,1..>585)
                     /citation=[8]
                     /note="colE1-kil mRNA"
     conflict        replace(83..84,"")
                     /citation=[5]
     conflict        replace(99..100,"")
                     /citation=[5]
     conflict        replace(135,"")
                     /citation=[5]
                     /note="replace 133..133 in Nucleic Acids Res. 6, 2647-2665
                     (1979)"
     conflict        replace(144,"")
                     /citation=[5]
                     /note="replace 142..142 in Nucleic Acids Res. 6, 2647-2665
                     (1979)"
     conflict        replace(174,"")
                     /citation=[5]
                     /note="replace 172..172 in Nucleic Acids Res. 6, 2647-2665
                     (1979)"
     conflict        replace((484.485)..(484.485),"")
                     /citation=[5]
                     /partial
                     /note="replace 101 in Nucleic Acids Res. 6, 2647-2665
                     (1979)"
     conflict        replace((491.492)..(491.492),"")
                     /citation=[5]
                     /partial
                     /note="replace 107 in Nucleic Acids Res. 6, 2647-2665
                     (1979)"
     conflict        replace((506.512)..(506.512),"")
                     /citation=[5]
                     /partial
                     /note="replace 121 in Nucleic Acids Res. 6, 2647-2665
                     (1979)"
     conflict        replace((517.519)..(517.519),"")
                     /citation=[5]
                     /partial
                     /note="replace 121..131 in Nucleic Acids Res. 6, 2647-2665
                     (1979)"
     conflict        replace(528,"")
                     /citation=[8]
                     /note="replace 528 in J. Biol. Chem. 260, 8925-8935
                     (1985)"
     misc_RNA        641..>641
                     /note="II RNA"
     misc_RNA        complement(645..751)
                     /note="I RNA ((3' end +/- 1 bp) [3]"
     conflict        replace((652.653)..(652.653),"")
                     /citation=[3]
                     /partial
                     /note="replace 31..32 in Eur. J. Biochem. 97, 435-443
                     (1979)"
     conflict        replace(1111,"")
                     /citation=[4]
                     /note="replace 1111 in Mol. Gen. Genet. 172, 151-159
                     (1979)"
     rep_origin      1197
                     /note="replication origin (+/- 1 bp) [1]"
     conflict        replace(1366,"")
                     /citation=[1]
                     /note="replace 359 in Proc. Natl. Acad. Sci. U.S.A. 74,
                     1865-1869 (1977)"
     conflict        replace((1416.1417)..(1416.1417),"")
                     /citation=[2]
                     /partial
                     /note="replace 7 in J. Mol. Biol. 124, 601-639 (1978)"
     mRNA            1449..>3936
                     /note="mob mRNA (5' end putative) [5],[8]; putative"
     conflict        replace(1454,"")
                     /citation=[2]
                     /note="replace 44 in J. Mol. Biol. 124, 601-639 (1978)"
     misc_feature    complement(1465..1466)
                     /note="relaxation (nic) cut site [2]"
     conflict        replace((1581.1582)..(1581.1582),"")
                     /citation=[2]
                     /partial
                     /note="replace 171 in J. Mol. Biol. 124, 601-639 (1978)"
     conflict        replace((4784.4787)..(4784.4787),"")
                     /citation=[6]
                     /partial
                     /note="replace 2..4.in Gene 15, 119-126 (1981)"
     misc_binding    5063..5093
                     /bound_moiety="LexA"
                     /citation=[8]
                     /note="LexA binding site"
     CDS             join(5140..6646,1..62)
                     /note="colicin E1 protein;  (cea)"
                     /codon_start=1
                     /translation="METAVAYYKDGVPYDDKGQVIITLLNGTPDGSGSGGGGGKGGSK
                     SESSAAIHATAKWSTAQLKKTQAEQAARAKAAAEAQAKAKANRDALTQRLKDIVNEAL
                     RHNASRTPSATELAHANNAAMQAEAERLRLAKAEEKARKEAEAAEKAFQEAEQRRKEI
                     EREKAETERQLKLAEAEEKRLAALSEEAKAVEIAQKKLSAAQSEVVKMDGEIKTLNSR
                     LSSSIHARDAEMKTLAGKRNELAQASAKYKELDELVKKLSPRANDPLQNRPFFEATRR
                     RVGAGKIREEKQKQVTASETRINRINADITQIQKAISQVSNNRNAGIARVHEAEENLK
                     KAQNNLLNSQIKDAVDATVSFYQTLTEKYGEKYSKMAQELADKSKGKKIGNVNEALAA
                     FEKYKDVLNKKFSKADRDAIFNALASVKYDDWAKHLDQFAKYLKITGHVSFGYDVVSD
                     ILKIKDTGDWKPLFLTLEKKAADAGVSYVVALLFSLLAGTTLGIWGIAIVTGILCSYI
                     DKNKLNTINEVLGI"
     CDS             complement(59..403)
                     /partial
                     /note="colicin E1 immunity protein;  (imm)"
                     /codon_start=1
                     /translation="FMSLRYYIKNILFGLYCTLIYIYLITKNSEGYYFLVSDKMLYAI
                     VISTILCPYSKYAIEYIAFNFIKKDFFERRKNLNNAPVAKLNLFMLYNLLCLVLAIPF
                     GLLGLFISIKNN"
     CDS             448..585
                     /note="kil protein"
                     /codon_start=1
                     /translation="MRKRFFVGIFAINLLVGCQANYIPDVQGGTIAPSSSSKLTGIAV
                     Q"
     CDS             605..895
                     /citation=[8]
                     /note="10.2 kd ORF"
                     /codon_start=1
                     /translation="LRSFFSARNLLLANKKTTATNGGLFAGSRATNSFSEGNWLQQSA
                     DTKYCPSSVAVVGPLLQELCSTVCAIIALLIRLPVAAASGVKACLTGLDSRR"
     CDS             complement(870..1241)
                     /citation=[8]
                     /note="13.8 kd ORF"
                     /codon_start=1
                     /translation="MSKRPGTGRRPRSRRFSIGSAPLTSITKIDAQVRGGETRQDYKA
                     TRRFPLEAPSCALLFRPCRLPDTCPPFSLREAWRFLIAHAVGISVRCRSFAPSWAVCT
                     NPPFSPTAAPYPVTIVLSPTR"
     CDS             1189..1350
                     /citation=[8]
                     /note="6.0 kd ORF"
                     /codon_start=1
                     /translation="MEKRLLRGLLPVPGLLLTCSFRPYPLILWITVLPFLCESVPLAA
                     VERPSVASQ"
     CDS             complement(1238..1408)
                     /citation=[8]
                     /note="6.4 kd ORF"
                     /codon_start=1
                     /translation="MPHRCVRRKCTSRRFSASSLTDSLRSVVRLRRAVLTHTKTVTQL
                     STESGDKAGKNM"
     CDS             1347..1526
                     /citation=[8]
                     /note="6.9 kd ORF"
                     /codon_start=1
                     /translation="MSEEAEKRLDVHFLLTHLCGISHPAWRTFHTIRTDAAWLSQYTL
                     RYRYVTGSGLRPDTR"
     CDS             complement(1500..1709)
                     /citation=[8]
                     /note="7.6 kd ORF"
                     /codon_start=1
                     /translation="MNRPIFVNRFTTTPMSFTAAASHVSGMTVKTSDTCSSRRRSQLV
                     CERMPGADKPVRARQQVLAGVGAQP"
     CDS             1609..1746
                     /note="mob1 protein;  (gtg start codon)"
                     /codon_start=1
                     /translation="MSEVFTVIPETCEAAAVKLIGVVVKRFTNIGLFICVQFVELLQQ
                     R"
     CDS             1609..1746
                     /note="mob1 ORF"
                     /codon_start=1
                     /translation="MSEVFTVIPETCEAAAVKLIGVVVKRFTNIGLFICVQFVELLQQ
                     R"
     CDS             complement(1612..1806)
                     /partial
                     /note="RNA I inhibition modulator protein;  (rom; gtg
                     start codon)"
                     /codon_start=1
                     /translation="SVTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHD
                     HADELYRSCLARFGDDGENL"
     CDS             1843..2190
                     /note="mob2 ORF"
                     /codon_start=1
                     /translation="MIPMKRERMLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPV
                     ARSGKLPTLAPPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRL
                     RLAVREQGARDDS"
     CDS             2180..3733
                     /note="mob3 ORF"
                     /codon_start=1
                     /translation="MIVKFHARGKGGGSGPVDYLLGRERNREGATVLQGIPEEVRELI
                     DATPFAKKYTSGVLSFAEKELPPGGREKVMASFERVLMPGLEKNQYSILWVEHQDKGR
                     LELNFVIPNMELQTGKRLQPYYDRADRPRIDAWQTLLNHHYGLHDPNAPENRRTLTLP
                     DNLPETKQALAEGVTRGIDALYHAGEIKGRQDVIQALTEAGLEVVRVTRSSISIADPN
                     GGKNIRLKGAFYEQSFADGRGVREKAERESRIYRENAEQRVQEARRICKRGCDIKRDE
                     NQRRYSPVHSLDRGIAGKTPGRGERGDDAAQEGRVKAGREYGHDVTGDSLSPVYREWR
                     DALVSWREDTGEPGRNQEAGRDIAETEREDMGRGVCAGREQEIPCPSVREISGGDSLS
                     GERVGTSEGVTQSDRAGNTFAERLRAAATGLYAAAERMGERLRGIAEDVFAYATGQRD
                     AERAGHAVESAGATLERADRTLEPVIQRELEIREERLIQEREHVLSLERERQPEIQER
                     TLDGPSLGW"
     CDS             2214..2399
                     /note="mob4 ORF"
                     /codon_start=1
                     /translation="MAVVRLITCWGGSVTAKAQRCFRVYRKKSGNSSMPRHLRRNTRP
                     VFCRSRRRSCRREDVKK"
     CDS             2868..3386
                     /note="mob6 ORF"
                     /codon_start=1
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNNEFRRLGESVSEAVTSN
                     ETKIRDAIALFTASTEESLEKHREGVKEAMMQHRRDVLKLAGNTGMMLLGIVFLLFTA
                     SGGTLWYLGGRIQANLEEIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVI
                     PFQGKEWVHLKE"
     CDS             3393..3626
                     /note="mob7 ORF"
                     /codon_start=1
                     /translation="MTELETHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDN
                     AMLNERVMQLSQQVQHLSEQTERLSQLYSENWR"
     CDS             3657..3818
                     /transl_except=(pos:3669..3671,aa:other)
                     /note="mob8 ORF;  Xxx"
                     /codon_start=1
                     /translation="MCYPXNGSVSRKYRNARWMALRWDGETMKNGSFSGLSGGNVACT
                     LWLHRYSYG"
     CDS             3766..3936
                     /transl_except=(pos:3847..3849,aa:other)
                     /note="mob9 ORF;  Xxx"
                     /codon_start=1
                     /translation="MGVMWPVPSGCIGIHTVKIYQARSRSFXGGLLPFLPVCCRDRAE
                     RVLAVRTIKGLW"
     CDS             complement(3943..4365)
                     /partial
                     /note="entry exclusion protein 2;  (exc2)"
                     /codon_start=1
                     /translation="LMRRTGNKLCLIAMITATVALTACTPKGSVEQHTRHYVYASDDG
                     FDPNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRG
                     TTQFAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
     CDS             4258..4647
                     /citation=[8]
                     /note="14.5 kd ORF"
                     /codon_start=1
                     /translation="MPGMLFHAALWGTGCESYCCCYHGDKTKFVSGTSHECLPTLKYL
                     FSVSYYPKTILSSVSDSVQRPNNLDQNPRFFSSSSACLSWAISFCSCSRSASWRLRSI
                     SCACLSSSISVRHCFSSFSSRRMRSVC"
     CDS             complement(4443..4865)
                     /partial
                     /note="entry exclusion protein 1;  (exc1)"
                     /codon_start=1
                     /translation="FMAWHTLRDALKMTGRSRSQFYRDMRGGLVSYRTGRDGRREFET
                     SELIRAYGELKQNETPERHSEGHAENPHDQQTERILRELNELKQCLTLMLEDKQAQDM
                     DRRRQEAEREQLQNEIAQLRQALELEKKRGFWSRLFGR"
     CDS             5435..5653
                     /citation=[8]
                     /note="8.8 kd ORF"
                     /codon_start=1
                     /translation="MRLFVTMPHVRLQQQSLLMLIMQLCRRKQSVCALRKQKKKPVKK
                     RKQQKRLFRKQNNDVKRLNGRRLKQNAS"
     CDS             5852..6013
                     /citation=[8]
                     /note="6.7 kd ORF"
                     /codon_start=1
                     /translation="MNWLRHPLNIKNWMSWSKNYHQEPMIRFRTVLFLKQPDDGLGPV
                     RLEKKNKNR"
BASE COUNT     1760 a   1421 c   1798 g   1667 t
ORIGIN      2 bp downstream of EcoRI site.
        1 ttctatgctc ctatattgat aagaataaac ttaatactat aaatgaggtg ttagggattt
       61 aattattctt tattgatata aaaagtccta gcaatccaaa tgggattgct aggaccaaac
      121 aaagtagatt atatagcata aataggttta attttgctac gggggcgtta tttaggtttt
      181 ttcttctttc gaaaaaatct ttctttatga agttaaaagc tatgtattca atagcatatt
      241 ttgaatatgg acatagaata gtgcttatca ctattgcata tagcatctta tctgacacaa
      301 ggaaataata cccttcgctg ttttttgtta taaggtatat atatataagt gtgcagtaca
      361 ggccaaataa aatatttttt atgtagtatc ttaagctcat aaattaaacc tcgccatata
      421 ttcttttcat tttataagga tcgagttatg aggaaaagat tttttgtggg aatattcgcg
      481 ataaacctcc ttgttggatg tcaggctaac tatatacctg atgttcaggg agggaccatc
      541 gcaccatcct cctcttctaa actgacgggg atcgcggttc agtagaaaag attaaaggat
      601 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
      661 taccaacggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
      721 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag tcgggccact
      781 acttcaagaa ctctgtagca ccgtttgtgc catcatcgct ctgctaatcc ggttaccagt
      841 ggctgctgcc agtggcgtta aggcgtgcct taccgggttg gactcaagac gatagttacc
      901 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
      961 aacgacctac accgaactga gataccaaca gcgtgagcta tgagaaagcg ccacgcttcc
     1021 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     1081 gagggagctt ccagggggaa acgcctggta gctttatagt cctgtcgggt ttcgccacct
     1141 ctgacttgag cgtctatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     1201 ctgctacgtg gccttcttcc tgttcctggt cttttgctca catgttcttt ccggccttat
     1261 cccctgattc tgtggataac tgtgttaccg tttttgtgtg agtcagtacc gctcgccgca
     1321 gtcgaacgac cgagcgtagc gagtcagtga gcgaggaagc ggaaaagcgc ctggacgtgc
     1381 attttctcct tacgcatctg tgcggcattt cacacccggc atggcgtact tttcatacaa
     1441 tccgcactga tgccgcatgg ttaagccagt atacactccg ctatcgctac gtgactgggt
     1501 cagggctgcg ccccgacacc cgctaaaacc tgctgacgcg ccctgacggg cttgtcagct
     1561 cccggcatcc gctcacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt
     1621 ttcaccgtca tccccgaaac gtgcgaggca gctgcggtaa agctcatcgg cgtggtcgtg
     1681 aagcgattca caaatatcgg cctgttcatc tgcgtccagt tcgttgagct tctccagcag
     1741 cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttagt
     1801 cactgatgcc tccgtgtaag ggggatttct gttcatgggg taatgatacc gatgaaacgc
     1861 gagaggatgc tcacaatacg ggttactgat gatgaacatg cccggttact tgaacgctgt
     1921 gagggtaaac aactggcggt atggatgcgg cgggtctgcc tgggggagcc ggttgcccgt
     1981 tccggaaaac tgccgacact ggcaccgccg ttactgcgtc agctggccgc catcggaaat
     2041 aacctgaatc agacagcccg taaggtgaac agcgggcagt ggtcttccgg tgaccgggtt
     2101 caggtggtgg ccgcactgat ggccatcggg gatgagctgc gccggctgcg tctggctgtc
     2161 agggaacagg gggcgcggga tgatagttaa atttcatgcc aggggaaaag gtggtggcag
     2221 tggtccggtt gattacctgc tggggaggga gcgtaaccgc gaaggcgcaa cggtgcttca
     2281 gggtataccg gaagaagtcc gggaactcat cgatgccacg ccatttgcga agaaatacac
     2341 gtccggtgtt ctgtcgttcg cggagaagga gctgccgccg ggaggacgtg aaaaagtgat
     2401 ggcgagcttt gagcgtgtac tgatgcccgg tctcgaaaaa aatcagtaca gcatcctgtg
     2461 ggtggagcac caggacaagg gacggcttga gctgaatttt gtcattccga acatggagct
     2521 acagaccgga aaacgcctcc agccgtacta cgaccgcgca gacaggccta gaattgatgc
     2581 ttggcagacg ctgttaaatc accattacgg gctgcatgac ccgaacgccc cggagaaccg
     2641 caggacgctg acactccctg ataacctgcc tgaaacgaaa caggcgcttg ctgagggcgt
     2701 cacgcgaggt atagatgcac tttaccatgc cggagagata aaaggccgtc aggatgtgat
     2761 tcaggcgctc actgaggcgg ggctggaagt ggtcagggtg acgcgaagca gtatcagcat
     2821 tgcagatccg aacggcggga agaatatcag gctgaaagga gcattttatg agcaatcttt
     2881 tgcagacggg cgcggagttc gagaaaaagc tgaaagagag agccgaatct acagagaaaa
     2941 tgctgaacaa cgagttcagg aggctcggcg aatctgtaag cgaggctgtg acatcaaacg
     3001 agacgaaaat cagagacgct atagccctgt tcacagcctc gacagaggaa tcgctggaaa
     3061 aacaccggga aggggtgaaa gaggcgatga tgcagcacag gagggacgtg ttaaagctgg
     3121 cagggaatac gggcatgatg ttactgggga tagtctttct cctgtttacc gcgagtggcg
     3181 ggacgctctg gtatcttgga gggaggatac aggcgaacct ggaagaaatc aggaagcagg
     3241 aagagacatt gcagaaactg aacgcgaaga catggggcgt ggagtttgtg caggacggga
     3301 acaggaaatt ccttgtcctt ccgtacggga aatcagcgga ggtgattccc tttcagggga
     3361 aagagtgggt acatctgaag gagtgacaca gagtgacaga gctggaaaca catttgctga
     3421 acgccttaga gcagctgcaa caggactata tgcagcggct gagcgaatgg gagagcgcct
     3481 tcgtggaatt gcagaagatg ttttcgctta cgcaacggga caacgcgatg ctgaacgagc
     3541 gggtcatgca gttgagtcag caggtgcaac acttgagcga gcagacagaa cgcttgagcc
     3601 agttatacag cgagaactgg agataagaga ggaacggctg atacaggagc gcgaacatgt
     3661 gttatcccta gaacgggagc gtcagccgga aatacaggaa cgcacgctgg atggcccttc
     3721 gctgggatgg tgaaaccatg aaaaatggca gcttcagtgg attaagtggg ggtaatgtgg
     3781 cctgtaccct ctggttgcat aggtattcat acggttaaaa tttatcaggc gcgatcgcgc
     3841 agtttttagg gtggtttgtt gccattttta cctgtctgct gccgtgatcg cgctgaacgc
     3901 gttttagcgg tgcgtacaat taagggatta tggtaaatcc acttactgtc tgccctcgta
     3961 gccatcgaga taaaccgcag aaatcgtgtc agccagcagc ctggattttt tcggggtaag
     4021 gtctttgctg cggtaatcag tacccgcaaa ttgagttgtg ccccggagtg agtttaaaaa
     4081 ttcttggctg tgaaactgct gaatgcgttg ttgcacatca ctccgtgatt ttcctgtcgc
     4141 tttgtcttta gctcccatat cccagaactg ccgaaaaaaa ggcaccatca ttcgtgttgt
     4201 gtcggctttt tgggtggaaa agttgggatc aaaaccgtca tcagaagcat atacgtaatg
     4261 ccgggtatgt tgttccacgc tgccctttgg ggtacaggct gtgagagcta ctgttgctgt
     4321 tatcatggcg ataagacaaa gtttgtttcc ggtacgtctc atgagtgtct ccctacctta
     4381 aagtatttat tctcggtgag ttattatccg aagactattt tatcatcagt ctctgacagc
     4441 gttcagcgac cgaacaacct ggaccagaat ccccgtttct tttccagttc cagtgcctgc
     4501 ctgagctggg ctatctcatt ttgtagctgt tcccgttctg cttcctggcg tctgcgatcc
     4561 atatcctgtg cctgtttatc ctcaagcatc agcgtcaggc attgtttcag ctcattcagt
     4621 tcccggagaa tgcgttctgt ctgctgatca tgtggatttt ctgcatgtcc ctcactgtgc
     4681 ctttctggtg tctcattctg ctttaattcg ccgtatgccc ggatcagttc actggtttca
     4741 aactcccgtc gtccgtccct gccggtacgg taactgacaa ggcctccgcg catatcacgg
     4801 taaaactgtg aacgcgatct gcctgtcatt tttagtgcgt cccggagtgt gtgccatgcc
     4861 ataaagtgac agtgtcccat agatgtctca tctcatagtt tcagtaaaac ataatgaggt
     4921 ctgagaacgg taatgtttgt gctggttttt gtggcatcgg gcgagaatag cgcgtggtgt
     4981 gaaagactgt ttttttgatc gttttcacaa aaatggaagt ccacagtctt gacagggaaa
     5041 atgcagcggc gtagctttta tgctgtatat aaaaccagtg gttatatgta cagtatttat
     5101 ttttaactta ttgttttaaa agtcaaagag gattttataa tggaaaccgc ggtagcgtac
     5161 tataaagatg gtgttcctta tgatgataag ggacaggtaa ttattactct tttgaatggt
     5221 actcctgacg ggagtggctc tggcggcgga ggtggaaaag gaggcagtaa aagtgaaagt
     5281 tctgcagcta ttcatgcaac tgctaaatgg tctactgctc aattaaagaa aacacaggca
     5341 gagcaggctg cccgggcaaa agctgcagcg gaagcacagg cgaaagcaaa ggcaaacagg
     5401 gatgcgctga ctcagcgcct gaaggatatc gtgaatgagg ctcttcgtca caatgcctca
     5461 cgtacgcctt cagcaacaga gcttgctcat gctaataatg cagctatgca ggcggaagca
     5521 gagcgtttgc gccttgcgaa agcagaagaa aaagcccgta aagaagcgga agcagcagaa
     5581 aaggcttttc aggaagcaga acaacgacgt aaagagattg aacgggagaa ggctgaaaca
     5641 gaacgccagt tgaaactggc tgaagctgaa gagaaacgac tggctgcatt gagtgaagaa
     5701 gctaaagctg ttgagatcgc ccaaaaaaaa ctttctgctg cacaatctga agtggtgaaa
     5761 atggatggag agattaagac tctcaattct cgtttaagct ccagtatcca tgcccgtgat
     5821 gcagaaatga aaacgctcgc tggaaaacga aatgaactgg ctcaggcatc cgctaaatat
     5881 aaagaactgg atgagctggt caaaaaacta tcaccaagag ccaatgatcc gcttcagaac
     5941 cgtccttttt ttgaagcaac cagacgacgg gttggggccg gtaagattag agaagaaaaa
     6001 caaaaacagg taacagcatc agaaacacgt attaaccgga taaatgctga tataactcag
     6061 atccagaagg ctatttctca ggtcagtaat aatcgtaatg ccggtatcgc tcgtgttcat
     6121 gaagctgaag aaaatttgaa aaaagcacag aataatctcc ttaattcaca gattaaggat
     6181 gctgttgatg caacagttag cttttatcaa acgctgactg aaaaatatgg tgaaaaatat
     6241 tcgaaaatgg cacaggaact tgctgataag tctaaaggta agaaaatcgg caatgtgaat
     6301 gaagctctcg ctgcttttga aaaatacaag gatgttttaa ataagaaatt cagcaaagcc
     6361 gatcgtgatg ctatttttaa tgcgttggca tcggtgaagt atgatgactg ggctaaacat
     6421 ttagatcagt ttgccaagta cttgaagatt acggggcatg tttcttttgg atatgatgtg
     6481 gtatctgata tcctaaaaat taaggataca ggtgactgga agccactatt tcttacatta
     6541 gagaagaaag ctgcagatgc aggggtgagt tatgttgttg ctttactttt tagcttgctt
     6601 gctggaacta cattaggtat ttggggtatt gctattgtta caggaa
//