From: GenBank-Updates@genbank.bio.net
Newsgroups: bionet.molbio.genbank.updates
Subject: Rhizobium leguminosarum nodulation-associated protein (rhiA, rhiB,
Date: 19 Aug 92 10:03:48 GMT
Distribution: bionet
Checksum: 14194     9

LOCUS       RHMRLV       4184 bp ds-DNA             BCT       19-AUG-1992
DEFINITION  Rhizobium leguminosarum nodulation-associated protein (rhiA, rhiB,
            rhi,C rhiR) genes, complete cds.
ACCESSION   M98835
KEYWORDS    rhiA gene; rhiB gene; rhiC gene; rhiR gene.
SOURCE      Rhizobium leguminosarum (sub_species viciae) DNA.
  ORGANISM  Rhizobium leguminosarum
            Prokaryotae; Gracilicutes; Scotobacteria; Rhizobiaceae.
REFERENCE   1  (bases 1 to 4184)
  AUTHORS   Cubo,M.T., Economou,A., Murphy,G.J., Johnston,A.W. and Downie,J.A.
  TITLE     Molecular characterization and regulation of the
            rhizophere-expressed gene rhiABCR that can influence nodulation by
            Rhizobium leguminosarum biovar viciae
  JOURNAL   J. Bacteriol. 174, 4026-4035 (1992)
  STANDARD  full staff_entry
FEATURES             Location/Qualifiers
     RBS             175..181
                     /gene="rhiA"
                     /note="putative"
     CDS             186..881
                     /gene="rhiA"
                     /codon_start=1
                     /translation="MSLHVSYVDKEMTDHARASQPGSAALAQGTQYSLLLKNQSAQPW
                     TFYVYQKMPQPVANVFSLAWFCSPYQIRVGNQIKFTWELAYNFVWSDTGQLIPGVDFF
                     ASGVEDCSPSGRNTTTFSLSDGPGLTAPIKGDPAGSLVINDAGNVPNNRFSVGIGMSG
                     TGTYVAQAGTNLLHTFTPTPSYWIAAGTNVTIGSVLSIDTITQTREAKFPSAVFNLVG
                     VLQEDNTWDINPA"
     RBS             916..923
                     /gene="rhiB"
                     /note="putative"
     CDS             931..1590
                     /gene="rhiB"
                     /codon_start=1
                     /translation="MGVPVVADSWHSFPHTSILPEEPTMFAGPPIGAVCPFAGQVAPI
                     SSSVNTIWSNTPCASSGEAAGTNAEAPISYVEAQGWMLCDGRYLRAAVYPELYAVLGG
                     LYGERNSTADLEFRIPDYRGLFLRGFDAGGGMDPDAKRRLDPTGNNVANVVGSLQCDA
                     LQVHAHPYEITTPAGISQQGNAAGTSISSKSTGSPENPARTALETRPKNVAVNYLIKF
                     R"
     RBS             1666..1673
                     /gene="rhiC"
                     /note="putative"
     CDS             1679..2128
                     /gene="rhiC"
                     /codon_start=1
                     /translation="MTATLRAFGWLAAFALTVTFAQGAAAEEQQKGKVGAKPVETGVV
                     IRGVTLAGPVGNPGTSTGKTCDFSGEPVDPSGRLEGASVNCRPNGNQANTTPGLPARF
                     NAYCMINAPVKSARLIQAARPENANHCDLSGITPKDATGQFGGAVWR"
     CDS             complement(2347..3090)
                     /gene="rhiR"
                     /codon_start=1
                     /translation="VKEESSAVSNLVFDFLSESASAKSKDDVLLLFGKISQYFGFSYF
                     AISGIPSPIERIDSYFVLGNWSVGWFDRYRENNYVHADPIVHLSKTCDHAFVWSEALR
                     DQKLDRQSRRVMDEAREFKLIDGFSVPLHTAAGFQSIVSFGAEKVELSTCDRSALYLM
                     AAYAHSLLRAQIGNDASRKIQALPMITTREREIIHWCAAGKTAIEIATILGRSHRTIQ
                     NVILNIQRKLNVVNTPQMIAESFRLRIIR"
                     /partial
     RBS             3096..3100
                     /gene="rhiR"
                     /note="putative"
BASE COUNT      977 a   1121 c   1144 g    942 t
ORIGIN
        1 ccctatgggt taatcgtggt ctgtcaggga gtaaatctac tcatttacgt cacccggaaa
       61 tgattatagc tgaattggtg tctccaataa cactcgcctc agttggagct gggccaagaa
      121 gcggtccaag cgattggatc agtcctggcg tgaagacaca aaatcaatgg aaaagggaga
      181 gttaaatgtc cttgcatgtc agctacgtag acaaggaaat gacggatcat gcccgtgcat
      241 cacagccggg aagcgcagcg cttgcccaag gaacgcaata ttcgttattg ctgaagaatc
      301 aatcggcgca accttggacc ttctatgtct atcaaaagat gcctcaacct gttgccaatg
      361 tcttctcctt ggcatggttc tgctctccgt atcaaattcg ggttggcaat caaatcaagt
      421 tcacttggga gctcgcctat aatttcgtct ggagcgacac gggacaactg attcctggcg
      481 tggacttttt tgcctccggg gtggaggact gcagccccag cgggagaaac accactactt
      541 tttcattaag cgatggacct ggcctgaccg cgccgatcaa gggggatcct gcaggatcac
      601 tggtcatcaa cgatgctggc aatgtgccaa acaaccggtt ctccgtgggc atcggcatgt
      661 ccggaacagg gacttatgtt gcacaagcgg gcaccaatct gctccacacg ttcacgccaa
      721 ctccgagcta ttggattgcg gcgggaacga atgtcactat cgggtcggtg ctcagcatcg
      781 acacgatcac ccagaccagg gaagccaagt ttccctctgc cgtcttcaat ctcgtaggcg
      841 tgctccaaga ggacaatacc tgggatatca acccggcttg atggcagggt gaattggctt
      901 cagctccgcc cgtcagggcg gaactgatgc atgggagtgc ctgttgttgc tgatagttgg
      961 cactcctttc ctcacacatc aatattgccc gaggaaccaa cgatgtttgc agggcctcca
     1021 atcggtgcgg tgtgtccctt tgcggggcaa gttgccccta tctcgagctc ggtcaataca
     1081 atctggagca acacgccctg cgcaagctct ggcgaggctg cgggtacaaa tgcggaagct
     1141 cctatcagct acgtcgaagc ccagggatgg atgctttgcg atggacgcta tctcagggca
     1201 gccgtctatc cagaactcta cgccgttctc ggcggactct atggagagcg gaattctacg
     1261 gccgatctgg aatttcggat tccagactac cgtgggctgt tcctgcgcgg tttcgatgcc
     1321 ggtggcggta tggaccctga cgcgaaacga cggctagatc caaccggcaa taatgttgcg
     1381 aatgttgttg gatcactcca atgcgatgct ctgcaagttc acgcgcatcc ctatgagata
     1441 acgacgccgg cgggaatttc gcagcagggc aatgccgccg gaacttccat ctcaagcaaa
     1501 tcgacaggtt cacccgaaaa tccggcacgc acagctcttg agacgcgtcc caagaacgtg
     1561 gccgtgaact acctgatcaa gtttcggtaa cttgaaaggg gtgtaggtcc tttcattcga
     1621 tttagagaca tcggtccgga agcgacatct tgccggccgg caaaagagga gaaccttaat
     1681 gactgctact ttgagagctt tcggatggct agctgctttc gctctgaccg tcacgttcgc
     1741 gcagggtgcc gcggctgagg agcagcaaaa aggcaaggtg ggggcgaagc ccgtagaaac
     1801 tggcgtcgtg atccgcggcg tcacgcttgc cggtccggtt ggaaatccgg gaacgtcaac
     1861 cgggaaaaca tgcgacttca gcggtgagcc cgtggatcca tcggggcgtt tggagggggc
     1921 aagcgtcaac tgcaggccaa atggcaacca ggcgaataca acgccagggc tacccgcacg
     1981 ctttaacgcg tattgcatga taaatgcgcc ggttaaaagt gcgcggctga tccaagctgc
     2041 ccgaccagaa aatgccaacc actgcgatct gtcggggatc accccaaaag acgcgactgg
     2101 tcaattcggg ggggccgtct ggcggtaggc ggtcattgga cgacttctgg ggaaacaaat
     2161 tcaaagcacg tcctcgcagc cccccaatcg atgctgcctg ctcgacctac cgaaaactgt
     2221 gttgcgcctg tctatcaatt cagacgcaga tttagctctg catttcaacg gggctactat
     2281 ctgatcatga tggctgtcgt gatccaacag gcccgatctt tgaagtggcc gaaaactcca
     2341 tatgcttcag cgaatgattc tgagacgaaa gctttcggca atcatctgag gtgtattaac
     2401 cacattgagc tttcgctgga tgttcaaaat gacattttga atggttcgat gggatcgtcc
     2461 gagaatcgtt gcgatctcta ttgctgtttt gcctgcggca caccaatgga tgatttcgcg
     2521 ctctcgcgtt gtgatcatgg gtaaggcctg aatttttcga ctggcatcat tcccaatttg
     2581 ggcgcggagc aagctgtgcg cgtaagccgc catcagatag agcgcgcttc ggtcgcaggt
     2641 tgagagctct accttttccg ctccaaaact gacaatcgat tgaaacccag cggcagtgtg
     2701 taaaggaacg ctgaagccgt caatcaactt gaattcgcgt gcctcatcca tgacacgacg
     2761 gctctgccga tcgagttttt gatctcgcag agcttccgac caaacaaatg cgtgatcgca
     2821 tgtcttgcta agatgcacta tggggtcggc gtggacataa ttgttttcac gataacgatc
     2881 aaaccacccg actgaccaat tgcccaaaac gaaataggaa tcaattcgct caataggcga
     2941 ggggatgccg gatatcgcga aataactaaa cccgaaatac tgcgaaattt ttccaaataa
     3001 gagtaacaca tcgtctttgc tctttgcgct ggctgactct gacaggaaat cgaacaccag
     3061 attcgagacc gctgaggatt cttccttcac gacaacctcc cggttcggtg caagattaac
     3121 ttcctcacat ctaagcagtg agtgtccagt tccgtgggca gctaaactct gaaaaccata
     3181 ttatataccc ttgatgcata caaccaaaaa gcgcatcttc aggcgggatc gtcctcggca
     3241 aaccgtttgg cgccgctcgt ctgaaaaaca attcgagccg tcaattttaa ccgagtgcgt
     3301 cagcccgatc gccatagctt cgcattcact cggaaccgta cggcagacga ctgagaactt
     3361 caaagcaggg cctgtcgcaa attttggcgc tcaacggtat gttgacatgg gaagatgatt
     3421 gccgttccga aagttgtgga gcgagccgat gattctgaat gctattgccg aaaagctgaa
     3481 gcggcaatcg atggatggtt tcaagcgccg gcattgttcg aggcatggct gatcgtgcgg
     3541 gcggttgcct tgtatttggg ttttgctcaa ggcttcagac gaattcgtca gaagcgaaag
     3601 tttaggcccg cctcaattac attatcgttg aggtcggacc ttgagctgat gcctccactc
     3661 gtggtcggga cggcgttgtt aatgacgtag ttgtactcga tctttgccga cacgccgccc
     3721 gaaaacgcgt gctcgacgcc gccaccaaag aggtagccgt gtttccagct atccggcccg
     3781 gatttccatt tgtccgatcc ggatacgagg cggtggccct cgaactcggt cgttgtgacg
     3841 cccgcggtgc cgtagacgaa agtgcgatca aagcccaagc cagccttcgc tttcgcggca
     3901 cctcggaagc ggctttccac gttgccgacg ggcacacgta cttcgttgtt caagtaagag
     3961 ccttccagtt ctgcgccgaa aacgcccgat ccagcctgga aattgtaacc tacctgagcg
     4021 cccaccgctg gggcgcgacc attgttgaac gggttgaatt tggaggccgc gacacctcca
     4081 tgaagaccgg cgtaagcccc ggcccacgag aaggttcccg gtgcgtcata agagggggcg
     4141 ctcggaacgc tgtggtcagc tagatcggcc gccgacgcgc cggg
//