Reference number : GC-2017-Q4-00237, amendment number : , agreement type : G, recipient legal name : Bourgault, Steve (Université du Québec à Montréal), recipient country : CA, recipient province : nan, recipient city : Quebec|Québec, recipient postal code : nan, program name english : Engage Grants for universities, program name french : Subventions d'engagement partenarial pour les universités, program purpose english : NMX Research and Solutions (NMX) is a Canadian translational research organization that provides_x000D_
development for drug discovery, including medicinal chemistry for leads and biophysical screening for hits._x000D_
The main biophysical strategies of NMX is nuclear magnetic resonance (NMR), which provides atomic-level_x000D_
information about compound solution properties and interactions with target biomolecules. Over the last years,_x000D_
NMX has developed a unique toolbox of NMR strategies to ensure delivery of quality hits and leads. These_x000D_
NMR-based integrated strategies for ligand screening for drug discovery now need to be supported with_x000D_
state-of-the-art biophysical techniques. In this context, NMX has contacted Dr. Steve Bourgault to initiate a_x000D_
collaboration in order to support their innovative NMR methods for quantitative determination of small_x000D_
molecule binding affinity and binding kinetics. The analytical biochemistry laboratory of Dr. Bourgault at_x000D_
UQÀM has developed over the last years an expertise in the biophysical analysis of biomolecular interactions,_x000D_
including isothermal titration calorimetry (ITC) and surface plasmon resonance (SPR)._x000D_
In this project, we will develop SPR and ITC strategies in parallel with NMR-based fragment-based lead_x000D_
discovery strategies through the screening of small molecules to identify ligands of galectin-7._x000D_
SPR and ITC are state-of-the-art techniques for studying molecular interactions and for measuring binding_x000D_
affinity (Kd). However, the screening of the binding of low-molecular weight compounds, as used in_x000D_
fragment-based lead discovery strategies, is very challenging and requires innovative techniques and many_x000D_
rounds of optimisation. Moreover, many drug targets self-assemble into oligomeric states (dimer, tetramer) in_x000D_
solution, as for galectin-7. This makes any attempts to correlate the results obtained from different biophysical_x000D_
methods all but trivial. Using our expertise in the characterization of molecular interactions, we will support_x000D_
NMX in the development of their unique NMR-based screening platform and we will gain a unique and_x000D_
unprecedented direct correlation between these biophysical techniques., program purpose french : NMX Research and Solutions (NMX) is a Canadian translational research organization that provides_x000D_
development for drug discovery, including medicinal chemistry for leads and biophysical screening for hits._x000D_
The main biophysical strategies of NMX is nuclear magnetic resonance (NMR), which provides atomic-level_x000D_
information about compound solution properties and interactions with target biomolecules. Over the last years,_x000D_
NMX has developed a unique toolbox of NMR strategies to ensure delivery of quality hits and leads. These_x000D_
NMR-based integrated strategies for ligand screening for drug discovery now need to be supported with_x000D_
state-of-the-art biophysical techniques. In this context, NMX has contacted Dr. Steve Bourgault to initiate a_x000D_
collaboration in order to support their innovative NMR methods for quantitative determination of small_x000D_
molecule binding affinity and binding kinetics. The analytical biochemistry laboratory of Dr. Bourgault at_x000D_
UQÀM has developed over the last years an expertise in the biophysical analysis of biomolecular interactions,_x000D_
including isothermal titration calorimetry (ITC) and surface plasmon resonance (SPR)._x000D_
In this project, we will develop SPR and ITC strategies in parallel with NMR-based fragment-based lead_x000D_
discovery strategies through the screening of small molecules to identify ligands of galectin-7._x000D_
SPR and ITC are state-of-the-art techniques for studying molecular interactions and for measuring binding_x000D_
affinity (Kd). However, the screening of the binding of low-molecular weight compounds, as used in_x000D_
fragment-based lead discovery strategies, is very challenging and requires innovative techniques and many_x000D_
rounds of optimisation. Moreover, many drug targets self-assemble into oligomeric states (dimer, tetramer) in_x000D_
solution, as for galectin-7. This makes any attempts to correlate the results obtained from different biophysical_x000D_
methods all but trivial. Using our expertise in the characterization of molecular interactions, we will support_x000D_
NMX in the development of their unique NMR-based screening platform and we will gain a unique and_x000D_
unprecedented direct correlation between these biophysical techniques., agreement title english : Development of biophysical assays for fragment-based lead discovery strategies, agreement title french : Development of biophysical assays for fragment-based lead discovery strategies, agreement number : EGP, agreement value : 25000.0, agreement start date : 2018-03-07, agreement end date : nan, description english : nan, description french : nan, expected results english : nan, expected results french : nan, additional information english : Grant or Award spanning more than one fiscal year (2017-2018 to 2018-2019)., additional information french : Subvention ou bourse octroyée s'appliquant à plus d'un exercice financier (2017-2018 à 2018-2019)., owner organization : nserc-crsng, owner organization title : Natural Sciences and Engineering Research Council of Canada | Conseil de recherches en sciences naturelles et en génie du Canada