Reference number : GC-2017-Q1-00377, amendment number : , agreement type : G, recipient legal name : Benfey, Tillmann (University of New Brunswick)|Benfey, Tillmann (Université du Nouveau-Brunswick), recipient country : CA, recipient province : nan, recipient city : New Brunswick|Nouveau-Brunswick, recipient postal code : nan, program name english : Engage Grants for Universities, program name french : Subventions d'engagement partenarial pour les universités, program purpose english : Cryogenetics Services Inc. provides services and technology for short- and long-term storage of fish sperm for_x000D_
research, conservation and aquaculture applications, and they have an interest in applying this technology to_x000D_
zebrafish because of the growing importance of this model species for biomedical research. As an extension of_x000D_
this, they also wish to develop technologies for short- and long-term storage of zebrafish eggs and embryos. To_x000D_
support their zebrafish program, Cryogenetics requires information on (1) the best rearing protocols to prime_x000D_
adult zebrafish for breeding, and (2) the best protocols for collecting and storing zebrafish eggs prior to_x000D_
fertilization. Having captive females produce high quality eggs at a predictable frequency requires optimizing_x000D_
their abiotic and biotic rearing conditions. The objectives of this project are therefore to develop protocols for_x000D_
maximizing egg production frequency and quality in zebrafish and to optimize the use of extender solution for_x000D_
in vitro fertilization. Research will focus on determining optimum adult feeding amount and frequency,_x000D_
between-spawning time interval, within-tank sex ratio, use of hormones to induce ovulation, and_x000D_
egg-to-extender ratio for storing eggs prior to fertilization., program purpose french : Cryogenetics Services Inc. provides services and technology for short- and long-term storage of fish sperm for_x000D_
research, conservation and aquaculture applications, and they have an interest in applying this technology to_x000D_
zebrafish because of the growing importance of this model species for biomedical research. As an extension of_x000D_
this, they also wish to develop technologies for short- and long-term storage of zebrafish eggs and embryos. To_x000D_
support their zebrafish program, Cryogenetics requires information on (1) the best rearing protocols to prime_x000D_
adult zebrafish for breeding, and (2) the best protocols for collecting and storing zebrafish eggs prior to_x000D_
fertilization. Having captive females produce high quality eggs at a predictable frequency requires optimizing_x000D_
their abiotic and biotic rearing conditions. The objectives of this project are therefore to develop protocols for_x000D_
maximizing egg production frequency and quality in zebrafish and to optimize the use of extender solution for_x000D_
in vitro fertilization. Research will focus on determining optimum adult feeding amount and frequency,_x000D_
between-spawning time interval, within-tank sex ratio, use of hormones to induce ovulation, and_x000D_
egg-to-extender ratio for storing eggs prior to fertilization., agreement title english : Optimizing egg production for in vitro fertilization in zebrafish, agreement title french : Optimizing egg production for in vitro fertilization in zebrafish, agreement number : EGP, agreement value : 21500.0, agreement start date : 2017-06-14, agreement end date : nan, description english : nan, description french : nan, expected results english : nan, expected results french : nan, additional information english : Grant or Award spanning more than one fiscal year. (2017-2018 to 2018-2019), additional information french : Subvention ou bourse octroyée s'appliquant à plus d'un exercice financier. (2017-2018 à 2018-2019), owner organization : nserc-crsng, owner organization title : Natural Sciences and Engineering Research Council of Canada | Conseil de recherches en sciences naturelles et en génie du Canada