Reference number : GC-2017-Q1-00481, amendment number : , agreement type : G, recipient legal name : Chen, Jennifer I-Ling (York University)|Chen, Jennifer I-Ling (Université York), recipient country : CA, recipient province : nan, recipient city : Ontario, recipient postal code : nan, program name english : Engage Grants for Universities, program name french : Subventions d'engagement partenarial pour les universités, program purpose english : The core technology of Fluidigm Canada Inc. is mass cytometry that enables real-time quantitative analysis of_x000D_
biomarkers in single cells. The technology is premised on the use of stable isotopes as labels whereby the_x000D_
tagged cells are introduced into an inductively coupled plasma and the ion cloud is analyzed by time-of-flight_x000D_
mass spectrometry. Amplification methods based on a cascaded hybridization assay are employed for analyzing_x000D_
messenger RNA and proteins; however, this approach is ineffective for short nucleic acid targets such as_x000D_
microRNA. Hence alternative signal amplification strategies with high specificity and high detection signals_x000D_
are of interest. The proposed work investigates DNA-functionalized nanoparticles that will cross-link in vitro_x000D_
in the presence of microRNA as probes for mass cytometry. The cellular uptake, cross-linking and subsequent_x000D_
excretion or removal of the nanoparticles will be investigated. With each nanoparticle consisting of thousands_x000D_
of atoms, variations in the number of nanoparticles in cells as a result of differences in the extent of_x000D_
aggregation will give large differences in the signal. The correlation between microRNA level and mass_x000D_
cytometry signal will be examined. The project will expand the applicability of mass cytometry beyond_x000D_
proteomics and messenger RNA and further empower biomedical research that investigates the role of miRNA_x000D_
in various diseases, including cancer, pre-eclampsia and cardio vascular diseases., program purpose french : The core technology of Fluidigm Canada Inc. is mass cytometry that enables real-time quantitative analysis of_x000D_
biomarkers in single cells. The technology is premised on the use of stable isotopes as labels whereby the_x000D_
tagged cells are introduced into an inductively coupled plasma and the ion cloud is analyzed by time-of-flight_x000D_
mass spectrometry. Amplification methods based on a cascaded hybridization assay are employed for analyzing_x000D_
messenger RNA and proteins; however, this approach is ineffective for short nucleic acid targets such as_x000D_
microRNA. Hence alternative signal amplification strategies with high specificity and high detection signals_x000D_
are of interest. The proposed work investigates DNA-functionalized nanoparticles that will cross-link in vitro_x000D_
in the presence of microRNA as probes for mass cytometry. The cellular uptake, cross-linking and subsequent_x000D_
excretion or removal of the nanoparticles will be investigated. With each nanoparticle consisting of thousands_x000D_
of atoms, variations in the number of nanoparticles in cells as a result of differences in the extent of_x000D_
aggregation will give large differences in the signal. The correlation between microRNA level and mass_x000D_
cytometry signal will be examined. The project will expand the applicability of mass cytometry beyond_x000D_
proteomics and messenger RNA and further empower biomedical research that investigates the role of miRNA_x000D_
in various diseases, including cancer, pre-eclampsia and cardio vascular diseases., agreement title english : Nanoparticles as probes for imaging mass-cytometry analysis of microRNA, agreement title french : Nanoparticles as probes for imaging mass-cytometry analysis of microRNA, agreement number : EGP, agreement value : 25000.0, agreement start date : 2017-06-14, agreement end date : nan, description english : nan, description french : nan, expected results english : nan, expected results french : nan, additional information english : Grant or Award spanning more than one fiscal year. (2017-2018 to 2018-2019), additional information french : Subvention ou bourse octroyée s'appliquant à plus d'un exercice financier. (2017-2018 à 2018-2019), owner organization : nserc-crsng, owner organization title : Natural Sciences and Engineering Research Council of Canada | Conseil de recherches en sciences naturelles et en génie du Canada