Reference number : GC-2017-Q2-00472, amendment number : , agreement type : G, recipient legal name : Nabi, Ivan (The University of British Columbia), recipient country : CA, recipient province : nan, recipient city : British Columbia|Colombie-Britannique, recipient postal code : nan, program name english : Idea to Innovation, program name french : De l'idée à l'innovation, program purpose english : Super-resolution microscopy has broken the optical diffraction barrier, enabling biologists to visualize_x000D_
molecular structures at resolutions approaching that of an electron microscope. The best resolution (XY:_x000D_
<20nm; Z: <50nm) is obtained using single molecule localization microscope (SMLM), based on the repeated_x000D_
activation (blinking) of discrete fluorophores whose precise localization is determined using a Gaussian fit of_x000D_
the point-spread function (PSF). By modeling 3D single-molecule localization data sets as a point cloud, a_x000D_
well-established representation used in 3D visual computing, we established analytical approaches to define the_x000D_
structure of macromolecular complexes in intact cells. Unsupervised clustering analysis and multi-threshold_x000D_
modularity analysis of caveolin-1, a key structural component of ~100 nm caveolae, has defined the modular_x000D_
nature of the caveolae coat structure and shows that smaller non-caveolar Cav1 scaffolds interact to form larger_x000D_
scaffolds and caveolae. We have initiated discussion with Quorum Technologies Inc to refine our software,_x000D_
providing comprehensive documentation and a clear user interface, to develop a stand-alone package for use_x000D_
with any SMLM microscope system. This application is to support a market assessment through Adventus_x000D_
Research + Consulting Inc (Guelph, Ontario) to assess the commercial viability of this technology including_x000D_
validation of the potential market size for the technology, validation of the end-user need and_x000D_
willingness/ability to purchase. Application of computational imaging, network modelling, and machine_x000D_
learning based 3D pattern analysis tools to SMLM represents a highly innovative and powerful approach to_x000D_
define molecular structure and molecular interactions in subcellular structures that will lead to the_x000D_
multidisciplinary training of HQP in this burgeoning field., program purpose french : Super-resolution microscopy has broken the optical diffraction barrier, enabling biologists to visualize_x000D_
molecular structures at resolutions approaching that of an electron microscope. The best resolution (XY:_x000D_
<20nm; Z: <50nm) is obtained using single molecule localization microscope (SMLM), based on the repeated_x000D_
activation (blinking) of discrete fluorophores whose precise localization is determined using a Gaussian fit of_x000D_
the point-spread function (PSF). By modeling 3D single-molecule localization data sets as a point cloud, a_x000D_
well-established representation used in 3D visual computing, we established analytical approaches to define the_x000D_
structure of macromolecular complexes in intact cells. Unsupervised clustering analysis and multi-threshold_x000D_
modularity analysis of caveolin-1, a key structural component of ~100 nm caveolae, has defined the modular_x000D_
nature of the caveolae coat structure and shows that smaller non-caveolar Cav1 scaffolds interact to form larger_x000D_
scaffolds and caveolae. We have initiated discussion with Quorum Technologies Inc to refine our software,_x000D_
providing comprehensive documentation and a clear user interface, to develop a stand-alone package for use_x000D_
with any SMLM microscope system. This application is to support a market assessment through Adventus_x000D_
Research + Consulting Inc (Guelph, Ontario) to assess the commercial viability of this technology including_x000D_
validation of the potential market size for the technology, validation of the end-user need and_x000D_
willingness/ability to purchase. Application of computational imaging, network modelling, and machine_x000D_
learning based 3D pattern analysis tools to SMLM represents a highly innovative and powerful approach to_x000D_
define molecular structure and molecular interactions in subcellular structures that will lead to the_x000D_
multidisciplinary training of HQP in this burgeoning field., agreement title english : Market research study proposal for an assessment of market opportunities for application of 3D pattern analysis to single-molecule localization microscopy (SMLM), agreement title french : Market research study proposal for an assessment of market opportunities for application of 3D pattern analysis to single-molecule localization microscopy (SMLM), agreement number : I2IPJ, agreement value : 15000.0, agreement start date : 2017-08-23, agreement end date : nan, description english : nan, description french : nan, expected results english : nan, expected results french : nan, additional information english : Grant or Award spanning more than one fiscal year (2017-2018 to 2018-2019)., additional information french : Subvention ou bourse octroyée s'appliquant à plus d'un exercice financier (2017-2018 à 2018-2019)., owner organization : nserc-crsng, owner organization title : Natural Sciences and Engineering Research Council of Canada | Conseil de recherches en sciences naturelles et en génie du Canada