Reference number : GC-2017-Q2-00081, amendment number : , agreement type : G, recipient legal name : Yan, Hongbin (Brock University), recipient country : CA, recipient province : nan, recipient city : Ontario, recipient postal code : nan, program name english : Engage Grants for Universities, program name french : Subventions d'engagement partenarial pour les universités, program purpose english : Oligonucleotide probes have found extensive applications in areas such as molecular biology, imaging, and_x000D_
diagnostics. These probes incorporate reporters such as fluorophores, chromophores, and other modified_x000D_
reporters that allow for the visualization of target nucleic acid sequences using a wide range of techniques._x000D_
While some oligonucleotide probes are prepared directly through the solid phase synthesis, many reporters are_x000D_
incompatible with the conditions that are used in this approach. As a result, post-synthetic modifications, i.e._x000D_
conjugation reactions, are performed after solid phase synthesis to covalently link the reporters to_x000D_
oligonucleotides. Post-synthetic modifications are often inefficient in terms of reaction kinetics and/or yields,_x000D_
and involve tedious purifications steps. ACGT Corporation has an invested interest in developing novel_x000D_
oligonucleotides through either direct incorporation by the phosphoramidite chemistry or post-synthetic_x000D_
modifications. The present proposal will develop protocols for the bioconjugation of oligonucleotides with_x000D_
probes through the azido-alkyn Click chemistry., program purpose french : Oligonucleotide probes have found extensive applications in areas such as molecular biology, imaging, and_x000D_
diagnostics. These probes incorporate reporters such as fluorophores, chromophores, and other modified_x000D_
reporters that allow for the visualization of target nucleic acid sequences using a wide range of techniques._x000D_
While some oligonucleotide probes are prepared directly through the solid phase synthesis, many reporters are_x000D_
incompatible with the conditions that are used in this approach. As a result, post-synthetic modifications, i.e._x000D_
conjugation reactions, are performed after solid phase synthesis to covalently link the reporters to_x000D_
oligonucleotides. Post-synthetic modifications are often inefficient in terms of reaction kinetics and/or yields,_x000D_
and involve tedious purifications steps. ACGT Corporation has an invested interest in developing novel_x000D_
oligonucleotides through either direct incorporation by the phosphoramidite chemistry or post-synthetic_x000D_
modifications. The present proposal will develop protocols for the bioconjugation of oligonucleotides with_x000D_
probes through the azido-alkyn Click chemistry., agreement title english : Development of modified oligonucleotide probes, agreement title french : Development of modified oligonucleotide probes, agreement number : EGP, agreement value : 25000.0, agreement start date : 2017-07-12, agreement end date : nan, description english : nan, description french : nan, expected results english : nan, expected results french : nan, additional information english : Grant or Award spanning more than one fiscal year (2017-2018 to 2018-2019)., additional information french : Subvention ou bourse octroyée s'appliquant à plus d'un exercice financier (2017-2018 à 2018-2019)., owner organization : nserc-crsng, owner organization title : Natural Sciences and Engineering Research Council of Canada | Conseil de recherches en sciences naturelles et en génie du Canada