Reference number : GC-2017-Q3-00451, amendment number : , agreement type : G, recipient legal name : Guan, Leluo (University of Alberta), recipient country : CA, recipient province : nan, recipient city : Alberta, recipient postal code : nan, program name english : Engage Grants for universities, program name french : Subventions d'engagement partenarial pour les universités, program purpose english : Cattle industry is one of the major Canadian agriculture sectors, which provides the animal proteins (milk and_x000D_
meat) to humans. Recently, the direct fed microbes have been considered as one of the powerful approaches to_x000D_
have long term effect to improve the animal productivity and health through enhancing microbial fermentation._x000D_
Biomate, manufactured by AB Mauri North America (LaSalle, QC) is one of the commercial available live_x000D_
yeast products (Saccharomyces cerevisiae variety) that can improve fiber digestion and prevent the ruminal_x000D_
metabolic dysfunction, resulting cattle with greater productivity and better health. On farm, the Biomate is_x000D_
usually mixed with the feed and provided to the animals through the day to achieve the maximum effect of the_x000D_
product. Therefore, it is essential to maintain the high level of the viable cells in feed. Currently, the evaluation_x000D_
of the activity of this product in feed uses the culture dependent methods. To evaluate the viable cells of yeast_x000D_
in feed, the cultivation of the yeast is usually performed at AB Mauri's lab, which takes 3-4 days that is time_x000D_
consuming and labor costly. Also, this method has a large error margin due to the manual nature of counting_x000D_
colonies. In addition, the cultivability of yeast sometime is affected by the high content of minerals or other_x000D_
components in feed, which results in an underestimation of viable yeast in the sample. Although the PCR_x000D_
method can be used to monitor the population of targeted organisms, it can't differentiate the proportion of_x000D_
non-viable and viable cells, which could lead to over-estimation of the viable cells in the sample. In this study,_x000D_
we propose to identify and target "marker genes" for viable yeast cells using microbial genomics and molecular_x000D_
biological based approaches. We will identify the DNA markers by aligning ~120 existing yeast genomes and_x000D_
then develop a single or multiplex PCR assay. The assay will then be validated using the mixed feed samples_x000D_
from AB Mauri, together with the comparison of the cultured based methods. The rapid and effective_x000D_
DNA/RNA based method will provide a reliable assay to quantify and to monitor the alive yeast cells in feed_x000D_
for AB Mauri lab., program purpose french : Cattle industry is one of the major Canadian agriculture sectors, which provides the animal proteins (milk and_x000D_
meat) to humans. Recently, the direct fed microbes have been considered as one of the powerful approaches to_x000D_
have long term effect to improve the animal productivity and health through enhancing microbial fermentation._x000D_
Biomate, manufactured by AB Mauri North America (LaSalle, QC) is one of the commercial available live_x000D_
yeast products (Saccharomyces cerevisiae variety) that can improve fiber digestion and prevent the ruminal_x000D_
metabolic dysfunction, resulting cattle with greater productivity and better health. On farm, the Biomate is_x000D_
usually mixed with the feed and provided to the animals through the day to achieve the maximum effect of the_x000D_
product. Therefore, it is essential to maintain the high level of the viable cells in feed. Currently, the evaluation_x000D_
of the activity of this product in feed uses the culture dependent methods. To evaluate the viable cells of yeast_x000D_
in feed, the cultivation of the yeast is usually performed at AB Mauri's lab, which takes 3-4 days that is time_x000D_
consuming and labor costly. Also, this method has a large error margin due to the manual nature of counting_x000D_
colonies. In addition, the cultivability of yeast sometime is affected by the high content of minerals or other_x000D_
components in feed, which results in an underestimation of viable yeast in the sample. Although the PCR_x000D_
method can be used to monitor the population of targeted organisms, it can't differentiate the proportion of_x000D_
non-viable and viable cells, which could lead to over-estimation of the viable cells in the sample. In this study,_x000D_
we propose to identify and target "marker genes" for viable yeast cells using microbial genomics and molecular_x000D_
biological based approaches. We will identify the DNA markers by aligning ~120 existing yeast genomes and_x000D_
then develop a single or multiplex PCR assay. The assay will then be validated using the mixed feed samples_x000D_
from AB Mauri, together with the comparison of the cultured based methods. The rapid and effective_x000D_
DNA/RNA based method will provide a reliable assay to quantify and to monitor the alive yeast cells in feed_x000D_
for AB Mauri lab., agreement title english : Development of a nucleic acid based assay to monitor and quantify viable yeast cells, agreement title french : Development of a nucleic acid based assay to monitor and quantify viable yeast cells, agreement number : EGP, agreement value : 25000.0, agreement start date : 2017-12-13, agreement end date : nan, description english : nan, description french : nan, expected results english : nan, expected results french : nan, additional information english : Grant or Award spanning more than one fiscal year (2017-2018 to 2018-2019)., additional information french : Subvention ou bourse octroyée s'appliquant à plus d'un exercice financier (2017-2018 à 2018-2019)., owner organization : nserc-crsng, owner organization title : Natural Sciences and Engineering Research Council of Canada | Conseil de recherches en sciences naturelles et en génie du Canada