Reference number : GC-2017-Q3-00509, amendment number : , agreement type : G, recipient legal name : De Buck, Jeroen (University of Calgary), recipient country : CA, recipient province : nan, recipient city : Alberta, recipient postal code : nan, program name english : Engage Grants for universities, program name french : Subventions d'engagement partenarial pour les universités, program purpose english : Maternal antibodies are crucial in the protection of calves against a large number of neonatal diseases. Maternal_x000D_
transfer of antibodies is restricted to the uptake of colostrum in contrast to other animals that are capable of_x000D_
placental transfer. Consequently, the concentrations of antibodies in colostrum is essential for the survival and_x000D_
well-being of the calves. Dairy producers have a choice of testing and storing high quality colostrum on farm or_x000D_
purchasing commercially available (e.g. Saskatoon Colostrum Company Ltd. (SSCL)) quality controlled freeze_x000D_
dried colostrum._x000D_
To produce uniform colostrum, SSCL needs to quantify the IgG levels of all the input colostrum batches and of_x000D_
their end-product. Currently, they are using an in-house Radial Immuno Diffusion assay which is slow and_x000D_
labor intensive._x000D_
In this project, we will adapt a novel total IgG assay to the needs and specification of SSCL's production_x000D_
processes. The novel assay which is based on a split enzyme fused to immunoglobulin binding protein_x000D_
domains. This assay has the advantage that no rinsing steps are required and that it has been previously_x000D_
demonstrated to correlate well with RID results. To eliminate any pipetting steps and thereby increase_x000D_
convenience and accuracy, the objectives of this project are to 1. Establish high through-put single step filter_x000D_
paper based colostrum IgG separation method compatible with the IgG quantification assay. 2. Compare and_x000D_
select the most practical and efficient version among different assay formats. 3. Production of all bioreagents_x000D_
by a single E.coli strain._x000D_
The projected reduction of sample processing and increased speed of test, will allow SSCL to significantly_x000D_
ramp up its colostrum testing capacity. The low-cost materials, ease of production of the bio-reagents and the_x000D_
lack of need for specialised equipment, which will make this a very inexpensive test system. This will help_x000D_
SSCL reduce the production costs and be more profitable and competitive., program purpose french : Maternal antibodies are crucial in the protection of calves against a large number of neonatal diseases. Maternal_x000D_
transfer of antibodies is restricted to the uptake of colostrum in contrast to other animals that are capable of_x000D_
placental transfer. Consequently, the concentrations of antibodies in colostrum is essential for the survival and_x000D_
well-being of the calves. Dairy producers have a choice of testing and storing high quality colostrum on farm or_x000D_
purchasing commercially available (e.g. Saskatoon Colostrum Company Ltd. (SSCL)) quality controlled freeze_x000D_
dried colostrum._x000D_
To produce uniform colostrum, SSCL needs to quantify the IgG levels of all the input colostrum batches and of_x000D_
their end-product. Currently, they are using an in-house Radial Immuno Diffusion assay which is slow and_x000D_
labor intensive._x000D_
In this project, we will adapt a novel total IgG assay to the needs and specification of SSCL's production_x000D_
processes. The novel assay which is based on a split enzyme fused to immunoglobulin binding protein_x000D_
domains. This assay has the advantage that no rinsing steps are required and that it has been previously_x000D_
demonstrated to correlate well with RID results. To eliminate any pipetting steps and thereby increase_x000D_
convenience and accuracy, the objectives of this project are to 1. Establish high through-put single step filter_x000D_
paper based colostrum IgG separation method compatible with the IgG quantification assay. 2. Compare and_x000D_
select the most practical and efficient version among different assay formats. 3. Production of all bioreagents_x000D_
by a single E.coli strain._x000D_
The projected reduction of sample processing and increased speed of test, will allow SSCL to significantly_x000D_
ramp up its colostrum testing capacity. The low-cost materials, ease of production of the bio-reagents and the_x000D_
lack of need for specialised equipment, which will make this a very inexpensive test system. This will help_x000D_
SSCL reduce the production costs and be more profitable and competitive., agreement title english : Fast and convenient assay for quantifying total immunoglobulin levels in cattle serum and colostrum, agreement title french : Fast and convenient assay for quantifying total immunoglobulin levels in cattle serum and colostrum, agreement number : EGP, agreement value : 25000.0, agreement start date : 2017-12-13, agreement end date : nan, description english : nan, description french : nan, expected results english : nan, expected results french : nan, additional information english : Grant or Award spanning more than one fiscal year (2017-2018 to 2018-2019)., additional information french : Subvention ou bourse octroyée s'appliquant à plus d'un exercice financier (2017-2018 à 2018-2019)., owner organization : nserc-crsng, owner organization title : Natural Sciences and Engineering Research Council of Canada | Conseil de recherches en sciences naturelles et en génie du Canada