Reference number : GC-2017-Q4-00758, amendment number : , agreement type : G, recipient legal name : Heath, Daniel (University of Windsor), recipient country : CA, recipient province : nan, recipient city : Ontario, recipient postal code : nan, program name english : Collaborative Research and Development Grants - Project, program name french : Subventions de recherche et développement coopérative - projet, program purpose english : The United States Coast Guard (USCG) recently introduced stringent regulations for the treatment of ballast_x000D_
water to minimize or eliminate the risk of the introduction of aquatic invasive species via ballast water_x000D_
transport. Ultra-violet (UV) light irradiation is a useful technology in ballast water treatment systems (BWTSs)_x000D_
for eliminating aquatic species which could be invasive and harmful to humans and the environment. UV_x000D_
damages DNA and prevents cellular replication, but the vital stain methods mandated in the USCG protocol_x000D_
may not detect UV damage in phytoplankton. Alternative culture-based measures of reproductive capacity are_x000D_
yet to be approved due to concerns over culture-based species biases (i.e., not all species may grow). Rapid and_x000D_
accurate assays to identify cells that are UV damaged beyond recovery are critical for the acceptance of_x000D_
UV-based BWTSs for phytoplankton. Hence we propose to develop high-throughput phytoplankton culture_x000D_
technologies and combine them with genomic/transcriptomic approaches to quantify DNA damage and the_x000D_
associated disruption of gene transcription at key functional gene loci across a range of gene function classes in_x000D_
the phytoplankton community typically found in ballast water._x000D_
Trojan Technologies (Trojan), headquartered in London, Ontario, Canada is one of the world's leading water_x000D_
treatment companies. The products and services provided by Trojan companies play vital roles in making_x000D_
various stages of the water treatment process more effective, efficient and sustainable, ranging from residential_x000D_
to industrial, municipal and marine applications. The application of UV sterilization technology to BWTSs_x000D_
represents a potential global market of >$15 billion (USD). We will partner with Trojan to develop the_x000D_
high-throughput culture protocols in concert with advanced meta-genomic and meta-transcriptomic technology_x000D_
to validate UV sterilization for BWTSs and thus expand Trojan's market on a global scale and provide the_x000D_
shipping industry with a practical, safe and cost-effective ballast water sterilization option., program purpose french : The United States Coast Guard (USCG) recently introduced stringent regulations for the treatment of ballast_x000D_
water to minimize or eliminate the risk of the introduction of aquatic invasive species via ballast water_x000D_
transport. Ultra-violet (UV) light irradiation is a useful technology in ballast water treatment systems (BWTSs)_x000D_
for eliminating aquatic species which could be invasive and harmful to humans and the environment. UV_x000D_
damages DNA and prevents cellular replication, but the vital stain methods mandated in the USCG protocol_x000D_
may not detect UV damage in phytoplankton. Alternative culture-based measures of reproductive capacity are_x000D_
yet to be approved due to concerns over culture-based species biases (i.e., not all species may grow). Rapid and_x000D_
accurate assays to identify cells that are UV damaged beyond recovery are critical for the acceptance of_x000D_
UV-based BWTSs for phytoplankton. Hence we propose to develop high-throughput phytoplankton culture_x000D_
technologies and combine them with genomic/transcriptomic approaches to quantify DNA damage and the_x000D_
associated disruption of gene transcription at key functional gene loci across a range of gene function classes in_x000D_
the phytoplankton community typically found in ballast water._x000D_
Trojan Technologies (Trojan), headquartered in London, Ontario, Canada is one of the world's leading water_x000D_
treatment companies. The products and services provided by Trojan companies play vital roles in making_x000D_
various stages of the water treatment process more effective, efficient and sustainable, ranging from residential_x000D_
to industrial, municipal and marine applications. The application of UV sterilization technology to BWTSs_x000D_
represents a potential global market of >$15 billion (USD). We will partner with Trojan to develop the_x000D_
high-throughput culture protocols in concert with advanced meta-genomic and meta-transcriptomic technology_x000D_
to validate UV sterilization for BWTSs and thus expand Trojan's market on a global scale and provide the_x000D_
shipping industry with a practical, safe and cost-effective ballast water sterilization option., agreement title english : Genomic, transcriptomic and culture-based assessment of UV irradiation effects on phytoplankton viability: applications for ballast water treatment systems, agreement title french : Genomic, transcriptomic and culture-based assessment of UV irradiation effects on phytoplankton viability: applications for ballast water treatment systems, agreement number : CRDPJ, agreement value : 150000.0, agreement start date : 2018-02-07, agreement end date : nan, description english : nan, description french : nan, expected results english : nan, expected results french : nan, additional information english : Grant or Award spanning more than one fiscal year (2017-2018 to 2020-2021)., additional information french : Subvention ou bourse octroyée s'appliquant à plus d'un exercice financier (2017-2018 à 2020-2021)., owner organization : nserc-crsng, owner organization title : Natural Sciences and Engineering Research Council of Canada | Conseil de recherches en sciences naturelles et en génie du Canada