Reference number : GC-2017-Q1-02617, amendment number : , agreement type : G, recipient legal name : Kamunde, Collins (University of Prince Edward Island), recipient country : CA, recipient province : nan, recipient city : Prince Edward Island|Île-du-Prince-Édouard, recipient postal code : nan, program name english : Discovery Grants Program - Individual, program name french : Programme de subventions à la découverte - individuelles, program purpose english : To effectively measure the interactive effects of multiple stressors on biological systems, a sensitive indicator of effects that integrates the responses induced by diverse stressors needs to be identified and characterized. My previous research suggests that oxidative stress acting on the mitochondria may be useful in integrating effects of multiple stressors. I therefore propose to probe the idea that diverse environmental stressors induce reactive oxygen species (ROS) leading to oxidative stress that impairs mitochondrial and cellular functions resulting in reduction in performance and fitness or death of the organism. I will test this idea using fish and three common and consequential stressors of aquatic systems: metals, temperature and hypoxia. To avoid shortcomings of previous research that have resulted in inconclusive findings because of investigating a limited number of endpoints, I will employ a holistic approach and interrogate ROS homeostasis from multiple angles. First, I will characterize mitochondrial and cellular ROS metabolism and redox regulation, and unveil how they are modulated by stressors. Second, I will examine how environmental stressors and their interactive effects influence mitochondrial homeostasis, and the role of ROS. Third, I will explore how environmental stressors modulate the relationship among different mitochondrial functional properties. Fourth, I will probe the molecular responses and oxidative modifications induced by ROS including how they are modulated by environmental stressors, and the functional consequences thereof. Overall, the proposed research will contribute fundamentally to our understanding of mitochondrial physiology and the oxidative stress response. Importantly, this research will advance our knowledge of how environmental stressors modulate the diverse effects of ROS that are believed to be the basis of many physiological and pathological states in animals. In practical terms, increased understanding of how combinations of stressors affect fitness of aquatic organisms will lead to better prediction and more effective management of their environmental impacts., program purpose french : To effectively measure the interactive effects of multiple stressors on biological systems, a sensitive indicator of effects that integrates the responses induced by diverse stressors needs to be identified and characterized. My previous research suggests that oxidative stress acting on the mitochondria may be useful in integrating effects of multiple stressors. I therefore propose to probe the idea that diverse environmental stressors induce reactive oxygen species (ROS) leading to oxidative stress that impairs mitochondrial and cellular functions resulting in reduction in performance and fitness or death of the organism. I will test this idea using fish and three common and consequential stressors of aquatic systems: metals, temperature and hypoxia. To avoid shortcomings of previous research that have resulted in inconclusive findings because of investigating a limited number of endpoints, I will employ a holistic approach and interrogate ROS homeostasis from multiple angles. First, I will characterize mitochondrial and cellular ROS metabolism and redox regulation, and unveil how they are modulated by stressors. Second, I will examine how environmental stressors and their interactive effects influence mitochondrial homeostasis, and the role of ROS. Third, I will explore how environmental stressors modulate the relationship among different mitochondrial functional properties. Fourth, I will probe the molecular responses and oxidative modifications induced by ROS including how they are modulated by environmental stressors, and the functional consequences thereof. Overall, the proposed research will contribute fundamentally to our understanding of mitochondrial physiology and the oxidative stress response. Importantly, this research will advance our knowledge of how environmental stressors modulate the diverse effects of ROS that are believed to be the basis of many physiological and pathological states in animals. In practical terms, increased understanding of how combinations of stressors affect fitness of aquatic organisms will lead to better prediction and more effective management of their environmental impacts., agreement title english : Modulation of reactive oxygen species and redox homeostasis by environmental stress, agreement title french : Modulation of reactive oxygen species and redox homeostasis by environmental stress, agreement number : RGPIN, agreement value : 195000.0, agreement start date : 2017-05-10, agreement end date : nan, description english : nan, description french : nan, expected results english : nan, expected results french : nan, additional information english : Grant or Award spanning more than one fiscal year. (2017-2018 to 2022-2023), additional information french : Subvention ou bourse octroyée s'appliquant à plus d'un exercice financier. (2017-2018 à 2022-2023), owner organization : nserc-crsng, owner organization title : Natural Sciences and Engineering Research Council of Canada | Conseil de recherches en sciences naturelles et en génie du Canada