Reference number : GC-2017-Q2-00548, amendment number : , agreement type : G, recipient legal name : Lee, Hyung-Sool (University of Waterloo), recipient country : CA, recipient province : nan, recipient city : Ontario, recipient postal code : nan, program name english : Collaborative Research and Development Grants - Project, program name french : Subventions de recherche et développement coopérative - projet, program purpose english : We will develop innovative biosensors using principles of microbial electrochemical cells (MECs) for measuring biochemical oxygen demand (BOD) concentration and enumerating the number of live E. coli in water and wastewater. Current methods for BOD measurement and enumeration of E. coli depend on culturing techniques, which are periodic, labour-intensive, and expensive. New MEC-based BOD sensors will be designed and optimized for synthetic and real wastewater. A prototype of the BOD sensors will be tested in wastewater treatment facilities and lakes through collaborations of the University of Waterloo and OZ Optics, Ltd. Field tests will allow us to assess the sensitivity and accuracy of the BOD sensors to fluctuations of water quality parameters in water and wastewater. In parallel, an innovative biosensor for counting live E. coli will be designed and tested to prove a hypothesis that electric current in MECs can represent the number of live E. coli. The success of the proposed research enables us to monitor BOD concentration and the number of live E. coli in water and wastewater as semi-continuous manners (data collection at every hour), which will significantly improve current water quality monitoring systems and help protection of water resources and human health against contaminants for Canada., program purpose french : We will develop innovative biosensors using principles of microbial electrochemical cells (MECs) for measuring biochemical oxygen demand (BOD) concentration and enumerating the number of live E. coli in water and wastewater. Current methods for BOD measurement and enumeration of E. coli depend on culturing techniques, which are periodic, labour-intensive, and expensive. New MEC-based BOD sensors will be designed and optimized for synthetic and real wastewater. A prototype of the BOD sensors will be tested in wastewater treatment facilities and lakes through collaborations of the University of Waterloo and OZ Optics, Ltd. Field tests will allow us to assess the sensitivity and accuracy of the BOD sensors to fluctuations of water quality parameters in water and wastewater. In parallel, an innovative biosensor for counting live E. coli will be designed and tested to prove a hypothesis that electric current in MECs can represent the number of live E. coli. The success of the proposed research enables us to monitor BOD concentration and the number of live E. coli in water and wastewater as semi-continuous manners (data collection at every hour), which will significantly improve current water quality monitoring systems and help protection of water resources and human health against contaminants for Canada., agreement title english : Development of innovative biosensors for biochemical oxygen demand and E. coli, agreement title french : Development of innovative biosensors for biochemical oxygen demand and E. coli, agreement number : CRDPJ, agreement value : 46155.0, agreement start date : 2017-08-23, agreement end date : nan, description english : nan, description french : nan, expected results english : nan, expected results french : nan, additional information english : Grant or Award spanning more than one fiscal year (2017-2018 to 2019-2020)., additional information french : Subvention ou bourse octroyée s'appliquant à plus d'un exercice financier (2017-2018 à 2019-2020)., owner organization : nserc-crsng, owner organization title : Natural Sciences and Engineering Research Council of Canada | Conseil de recherches en sciences naturelles et en génie du Canada