Reference number : GC-2017-Q1-00537, amendment number : , agreement type : G, recipient legal name : Nelson, Louise (The University of British Columbia), recipient country : CA, recipient province : nan, recipient city : British Columbia|Colombie-Britannique, recipient postal code : nan, program name english : Engage Plus Grants for Universities, program name french : Subvention d'engagement partenarial Plus pour les universités, program purpose english : Agrobacterium vitis is the causal agent of grapevine crown gall, an economically important disease in cold_x000D_
climate viticulture regions. The disease occurs frequently in Okanagan vineyards and limits grape production_x000D_
and lifespan of vineyards. The pathogen spreads either through infected grapevine nursery stock or infects_x000D_
healthy grapevines planted in soil harbouring A. vitis. Wounds in the grapevine trunk or canes due to winter_x000D_
freezing or mechanical injuries lead to the development of galls. In our earlier Engage grant-funded study we_x000D_
developed a rapid and sensitive protocol to quantify A. vitis in grapevine nursery stock. This allows us to_x000D_
screen grapevines that have been purchased by the grape industry before planting into a vineyard. To prevent_x000D_
infection of healthy stock from A. vitis residing in soil, a protocol to quantify the A. vitis soil population is_x000D_
urgently needed. Here we propose to expand the existing protocol to quantify A. vitis from virgin and vineyard_x000D_
soil. We will also determine the threshold concentration of A. vitis in soil that will result in the development of_x000D_
crown gall disease in a greenhouse experiment. This will lead to valuable information for growers to determine_x000D_
the risk of infection when a new vineyard is established. Our protocols will form the basis for the development_x000D_
of a biocontrol for crown gall disease in future projects., program purpose french : Agrobacterium vitis is the causal agent of grapevine crown gall, an economically important disease in cold_x000D_
climate viticulture regions. The disease occurs frequently in Okanagan vineyards and limits grape production_x000D_
and lifespan of vineyards. The pathogen spreads either through infected grapevine nursery stock or infects_x000D_
healthy grapevines planted in soil harbouring A. vitis. Wounds in the grapevine trunk or canes due to winter_x000D_
freezing or mechanical injuries lead to the development of galls. In our earlier Engage grant-funded study we_x000D_
developed a rapid and sensitive protocol to quantify A. vitis in grapevine nursery stock. This allows us to_x000D_
screen grapevines that have been purchased by the grape industry before planting into a vineyard. To prevent_x000D_
infection of healthy stock from A. vitis residing in soil, a protocol to quantify the A. vitis soil population is_x000D_
urgently needed. Here we propose to expand the existing protocol to quantify A. vitis from virgin and vineyard_x000D_
soil. We will also determine the threshold concentration of A. vitis in soil that will result in the development of_x000D_
crown gall disease in a greenhouse experiment. This will lead to valuable information for growers to determine_x000D_
the risk of infection when a new vineyard is established. Our protocols will form the basis for the development_x000D_
of a biocontrol for crown gall disease in future projects., agreement title english : Crown gall disease of grapevines: quantification of agrobacterium vitis in vineyard soil, agreement title french : Crown gall disease of grapevines: quantification of agrobacterium vitis in vineyard soil, agreement number : EGP2, agreement value : 9045.0, agreement start date : 2017-06-14, agreement end date : nan, description english : nan, description french : nan, expected results english : nan, expected results french : nan, additional information english : Grant or Award spanning more than one fiscal year. (2017-2018 to 2018-2019), additional information french : Subvention ou bourse octroyée s'appliquant à plus d'un exercice financier. (2017-2018 à 2018-2019), owner organization : nserc-crsng, owner organization title : Natural Sciences and Engineering Research Council of Canada | Conseil de recherches en sciences naturelles et en génie du Canada