Reference number : GC-2017-Q1-02667, amendment number : , agreement type : G, recipient legal name : Adamo, Kristi (University of Ottawa)|Adamo, Kristi (Université d’Ottawa), recipient country : CA, recipient province : nan, recipient city : Ontario, recipient postal code : nan, program name english : Discovery Grants Program - Individual, program name french : Programme de subventions à la découverte - individuelles, program purpose english : The long-term aim of my research program is to advance the basic understanding of the mechanistic functionality of the placenta, by identifying and characterizing biologically relevant exposures which alter placental biology at the molecular through to functional level. 
 
In Eutherian mammals, the placenta has evolved to become the physiological and metabolic link between mother and her offspring. Despite its integral role in sustaining new life, it is the least understood and least studied of all mammalian organs . The placenta, embedded in the uterine wall, is tethered by the umbilical cord to the fetus and acts as the interface of nutrient and oxygen transport, eliminates waste, regulates fetal temperature and produces hormones that promote appropriate maternal adaptation to pregnancy. Examining the response to challenges or the potential differences in form and function with varying environmental conditions (exercise or high energy expenditure for this program of research) in placenta tissue models is a highly novel research area. 
 
My team is interested in the impact of maternal energy expenditure on fundamental aspects of placenta biology. We hypothesize that the increases in energy expenditure that accompany exercise result in changes within the placenta related to vascular development and reactivity, substrate metabolism and mitochondrial form and function. The specific mechanisms underlying the optimized development and function of the placenta remain unknown. One mechanistic pathway through which maternal exercise may influence placenta biology is the myokine secretome &ndash; a collection of cytokines and small peptides secreted from contracting skeletal muscle, capable of paracrine and endocrine function. Since the placenta is the only mammalian organ that serves a vital function before exhausting its usefulness, it offers a large tissue repository to be leveraged for studies. In the proposed program of research one of our short-term objectives is to compare mechanistic functionality using 3rd trimester placenta explants, collected from women at the extreme ends of the energy expenditure spectrum (high vs. low EE) across pregnancy ( AIM 1 ). An additional short-term objective is to determine the specific contributions of myokine exposure, as biologically relevant stimuli mimicking exercise, on critical aspects of placenta biology using an ex vivo, 1st and 3rd trimester human placenta tissue explant model ( AIM 2 ). 
 
 IMPACT statement: Even though the placenta is arguably the most important life-supporting organ, we know very little about it. Understanding placenta function is thus critical as it impacts fetal phenotype and long-term physiological and metabolic development. Biologically, it is a fascinating tissue due to its rapid growth and subsequent demise along with its extremely broad range of functions., program purpose french : The long-term aim of my research program is to advance the basic understanding of the mechanistic functionality of the placenta, by identifying and characterizing biologically relevant exposures which alter placental biology at the molecular through to functional level. 
 
In Eutherian mammals, the placenta has evolved to become the physiological and metabolic link between mother and her offspring. Despite its integral role in sustaining new life, it is the least understood and least studied of all mammalian organs . The placenta, embedded in the uterine wall, is tethered by the umbilical cord to the fetus and acts as the interface of nutrient and oxygen transport, eliminates waste, regulates fetal temperature and produces hormones that promote appropriate maternal adaptation to pregnancy. Examining the response to challenges or the potential differences in form and function with varying environmental conditions (exercise or high energy expenditure for this program of research) in placenta tissue models is a highly novel research area. 
 
My team is interested in the impact of maternal energy expenditure on fundamental aspects of placenta biology. We hypothesize that the increases in energy expenditure that accompany exercise result in changes within the placenta related to vascular development and reactivity, substrate metabolism and mitochondrial form and function. The specific mechanisms underlying the optimized development and function of the placenta remain unknown. One mechanistic pathway through which maternal exercise may influence placenta biology is the myokine secretome &ndash; a collection of cytokines and small peptides secreted from contracting skeletal muscle, capable of paracrine and endocrine function. Since the placenta is the only mammalian organ that serves a vital function before exhausting its usefulness, it offers a large tissue repository to be leveraged for studies. In the proposed program of research one of our short-term objectives is to compare mechanistic functionality using 3rd trimester placenta explants, collected from women at the extreme ends of the energy expenditure spectrum (high vs. low EE) across pregnancy ( AIM 1 ). An additional short-term objective is to determine the specific contributions of myokine exposure, as biologically relevant stimuli mimicking exercise, on critical aspects of placenta biology using an ex vivo, 1st and 3rd trimester human placenta tissue explant model ( AIM 2 ). 
 
 IMPACT statement: Even though the placenta is arguably the most important life-supporting organ, we know very little about it. Understanding placenta function is thus critical as it impacts fetal phenotype and long-term physiological and metabolic development. Biologically, it is a fascinating tissue due to its rapid growth and subsequent demise along with its extremely broad range of functions., agreement title english : Characterization of placenta dynamics and response to various metabolic exposures, agreement title french : Characterization of placenta dynamics and response to various metabolic exposures, agreement number : RGPIN, agreement value : 125000.0, agreement start date : 2017-05-10, agreement end date : nan, description english : nan, description french : nan, expected results english : nan, expected results french : nan, additional information english : Grant or Award spanning more than one fiscal year. (2017-2018 to 2022-2023), additional information french : Subvention ou bourse octroyée s'appliquant à plus d'un exercice financier. (2017-2018 à 2022-2023), owner organization : nserc-crsng, owner organization title : Natural Sciences and Engineering Research Council of Canada | Conseil de recherches en sciences naturelles et en génie du Canada