Reference number : GC-2017-Q4-01270, amendment number : , agreement type : G, recipient legal name : Mine, Yoshinori (University of Guelph), recipient country : CA, recipient province : nan, recipient city : Ontario, recipient postal code : nan, program name english : Engage Grants for universities, program name french : Subventions d'engagement partenarial pour les universités, program purpose english : A significant amount of eggshell waste is produced as a by-product of the egg processing industry. Disposal of_x000D_
these eggshells creates an environmental and financial burden and, therefore, alternative uses for these_x000D_
materials would be of obvious benefit. Eggshell waste may be an important value-added resource for the_x000D_
extraction of novel bioactive peptides for human health, cosmetic, biomedical and nutraceutical applications. A_x000D_
critical step to the extraction of valuable eggshell-derived components is to achieve a clean separation of the_x000D_
membranes from the calcium-rich shell. While studies up until now have demonstrated that eggshell_x000D_
membrane-derived proteins may have bioactive/nutraceutical potential, little is known about eggshell_x000D_
membrane-derived peptides, including their production and potential bioactivity. The objectives: 1) To explore_x000D_
methods of eggshell membrane hydrolyzation capable of industrial production. 2) To study_x000D_
extraction/production and characterization of novel peptides from eggshell membrane waste and;3) To examine_x000D_
their use/applications in cosmetic products. Production of bioactive membrane-derived peptides will then be_x000D_
carried out by enzymatic hydrolysis using commercially available proteases, including collagenase and_x000D_
alcalase, to produce collagen-derived peptides/bioactive peptides. Different enzymes and enzyme combinations_x000D_
will be examined to determine the best method of peptide production. The potential activity of eggshell_x000D_
membrane peptides in cosmetic applications will also be examined using cultured skin cell lines (keratinocytes_x000D_
and fibroblasts). The proliferation of fibroblasts on the collagen gel by eggshell membrane derived peptides_x000D_
will be assessed in vitro. Fibroblasts migration and enzyme activity (hyuaronase, tyrocinase, elastase etc) will_x000D_
be analyzed using in vitro cell culture model. The Canadian egg industry generates over $1 billion into the_x000D_
economy, but it is vulnerable to shifts in consumer attitudes and perceptions about eggs. It is therefore_x000D_
important for the Canadian egg industry to diversify egg utilizations beyond traditional food uses and develop_x000D_
new value-added bio-products and heath products., program purpose french : A significant amount of eggshell waste is produced as a by-product of the egg processing industry. Disposal of_x000D_
these eggshells creates an environmental and financial burden and, therefore, alternative uses for these_x000D_
materials would be of obvious benefit. Eggshell waste may be an important value-added resource for the_x000D_
extraction of novel bioactive peptides for human health, cosmetic, biomedical and nutraceutical applications. A_x000D_
critical step to the extraction of valuable eggshell-derived components is to achieve a clean separation of the_x000D_
membranes from the calcium-rich shell. While studies up until now have demonstrated that eggshell_x000D_
membrane-derived proteins may have bioactive/nutraceutical potential, little is known about eggshell_x000D_
membrane-derived peptides, including their production and potential bioactivity. The objectives: 1) To explore_x000D_
methods of eggshell membrane hydrolyzation capable of industrial production. 2) To study_x000D_
extraction/production and characterization of novel peptides from eggshell membrane waste and;3) To examine_x000D_
their use/applications in cosmetic products. Production of bioactive membrane-derived peptides will then be_x000D_
carried out by enzymatic hydrolysis using commercially available proteases, including collagenase and_x000D_
alcalase, to produce collagen-derived peptides/bioactive peptides. Different enzymes and enzyme combinations_x000D_
will be examined to determine the best method of peptide production. The potential activity of eggshell_x000D_
membrane peptides in cosmetic applications will also be examined using cultured skin cell lines (keratinocytes_x000D_
and fibroblasts). The proliferation of fibroblasts on the collagen gel by eggshell membrane derived peptides_x000D_
will be assessed in vitro. Fibroblasts migration and enzyme activity (hyuaronase, tyrocinase, elastase etc) will_x000D_
be analyzed using in vitro cell culture model. The Canadian egg industry generates over $1 billion into the_x000D_
economy, but it is vulnerable to shifts in consumer attitudes and perceptions about eggs. It is therefore_x000D_
important for the Canadian egg industry to diversify egg utilizations beyond traditional food uses and develop_x000D_
new value-added bio-products and heath products., agreement title english : Value-added applications for industrial waste eggshell membrane for cosmetic application, agreement title french : Value-added applications for industrial waste eggshell membrane for cosmetic application, agreement number : EGP, agreement value : 25000.0, agreement start date : 2018-03-07, agreement end date : nan, description english : nan, description french : nan, expected results english : nan, expected results french : nan, additional information english : Grant or Award spanning more than one fiscal year (2017-2018 to 2018-2019)., additional information french : Subvention ou bourse octroyée s'appliquant à plus d'un exercice financier (2017-2018 à 2018-2019)., owner organization : nserc-crsng, owner organization title : Natural Sciences and Engineering Research Council of Canada | Conseil de recherches en sciences naturelles et en génie du Canada