Reference number : GC-2017-Q4-00663, amendment number : , agreement type : G, recipient legal name : Ghosh, Supratim (University of Saskatchewan), recipient country : CA, recipient province : nan, recipient city : Saskatchewan, recipient postal code : nan, program name english : Engage Grants for universities, program name french : Subventions d'engagement partenarial pour les universités, program purpose english : SM Research Inc. (SMR) wants to use a novel emulsion-based polymerase chain reaction (emPCR) technique_x000D_
to amplify DNA for their highly precise and accurate detection. The emPCR utilizes multiple thermal cycles of_x000D_
water-in-oil (W/O) emulsion where the water droplets act as micro-reactors for DNA This could significantly_x000D_
improve the efficiency of DNA amplification and allow detection of the extremely low concentration of DNA._x000D_
However, SMR is facing a significant problem with W/O emulsion destabilization during thermal cycles. In the_x000D_
proposed Engage research project, the researchers from U of S will develop advanced ingredient formulations_x000D_
using a fundamental scientific approach that will form the desired W/O emulsion in a simple, easy to use_x000D_
technology and remain stable under multiple thermal cycles. A three-step approach will be taken to address the_x000D_
technical complexities associated with the problem. The inherent thermodynamic destabilization of emulsion_x000D_
will be reduced by lowering of interfacial tension using a combination of different types of emulsifiers, while_x000D_
the breakage of the inter-droplet film will be prevented by increasing the interfacial elasticity. Finally, close_x000D_
approach of the droplets and their sedimentation will be prevented by increasing the viscosity of the continuous_x000D_
oil phase. This type of multi-step approach of prevention of emulsion thermal destabilization is novel and will_x000D_
undoubtedly lead to innovation in emulsion formulation and successful implementation in emPCR._x000D_
Implementation of this technology will be of tremendous benefit to SMR to establish themselves as a market_x000D_
leader and to secure global attention in a lucrative gene detection field. With this advanced technology,_x000D_
detection of critical, life-threatening disease could be done at an early stage, thus providing a significant benefit_x000D_
to Canadians for improved health and disease prevention., program purpose french : SM Research Inc. (SMR) wants to use a novel emulsion-based polymerase chain reaction (emPCR) technique_x000D_
to amplify DNA for their highly precise and accurate detection. The emPCR utilizes multiple thermal cycles of_x000D_
water-in-oil (W/O) emulsion where the water droplets act as micro-reactors for DNA This could significantly_x000D_
improve the efficiency of DNA amplification and allow detection of the extremely low concentration of DNA._x000D_
However, SMR is facing a significant problem with W/O emulsion destabilization during thermal cycles. In the_x000D_
proposed Engage research project, the researchers from U of S will develop advanced ingredient formulations_x000D_
using a fundamental scientific approach that will form the desired W/O emulsion in a simple, easy to use_x000D_
technology and remain stable under multiple thermal cycles. A three-step approach will be taken to address the_x000D_
technical complexities associated with the problem. The inherent thermodynamic destabilization of emulsion_x000D_
will be reduced by lowering of interfacial tension using a combination of different types of emulsifiers, while_x000D_
the breakage of the inter-droplet film will be prevented by increasing the interfacial elasticity. Finally, close_x000D_
approach of the droplets and their sedimentation will be prevented by increasing the viscosity of the continuous_x000D_
oil phase. This type of multi-step approach of prevention of emulsion thermal destabilization is novel and will_x000D_
undoubtedly lead to innovation in emulsion formulation and successful implementation in emPCR._x000D_
Implementation of this technology will be of tremendous benefit to SMR to establish themselves as a market_x000D_
leader and to secure global attention in a lucrative gene detection field. With this advanced technology,_x000D_
detection of critical, life-threatening disease could be done at an early stage, thus providing a significant benefit_x000D_
to Canadians for improved health and disease prevention., agreement title english : Development of thermally-stable water-in-oil emulsions for the creation of DNA micro-reactors in polymerase chain reaction, agreement title french : Development of thermally-stable water-in-oil emulsions for the creation of DNA micro-reactors in polymerase chain reaction, agreement number : EGP, agreement value : 25000.0, agreement start date : 2018-03-07, agreement end date : nan, description english : nan, description french : nan, expected results english : nan, expected results french : nan, additional information english : Grant or Award spanning more than one fiscal year (2017-2018 to 2018-2019)., additional information french : Subvention ou bourse octroyée s'appliquant à plus d'un exercice financier (2017-2018 à 2018-2019)., owner organization : nserc-crsng, owner organization title : Natural Sciences and Engineering Research Council of Canada | Conseil de recherches en sciences naturelles et en génie du Canada