Reference number : GC-2017-Q2-04291, amendment number : , agreement type : G, recipient legal name : Budman, Hector (University of Waterloo), recipient country : CA, recipient province : nan, recipient city : Ontario, recipient postal code : nan, program name english : Engage Plus Grants for Universities, program name french : Subvention d'engagement partenarial Plus pour les universités, program purpose english : Chinese hamster ovary (CHO) cells are used extensively in the pharmaceutical industry to produce recombinant_x000D_
proteins to treat an array of diseases. The productivity and the therapeutic effectiveness of these proteins are_x000D_
highly influenced by the chemical composition of the chemical medium and the bioreactor operating conditions_x000D_
that are used to grow the cells._x000D_
Optimizing the media composition is very challenging due to its complex nature involving tens of different_x000D_
components such as aminoacids and non-aminoacid species (salts, vitamins, metals etc.)._x000D_
Our industrial collaborator (Sigma-Aldrich), a main manufacturer of cell culture media, is currently_x000D_
experimenting with an advanced system of 48 micro-reactors (Sartorius-AMBR 15, 15 ml volume each), that it_x000D_
is not available in our lab, to develop new cell lines and growth media. Although industrial antibody_x000D_
manufacturing involves either fed-batch or perfusion operations, the AMBR micro-bioreactor system is_x000D_
comparatively much easier, quicker and cheaper to run for the purpose of media development._x000D_
The work of my group in modeling of mammalian cells (Nikdel and Budman, 2016) can be directly applied to_x000D_
two aspects that are of interest for Sigma for the purpose of media development with the AMBR system:_x000D_
i- To expand the validity of the model developed at Waterloo under the first Engage project for describing both_x000D_
AMBR and perfusion operations using different media formulations and_x000D_
ii- To develop a novel hybrid modeling approach, where the metabolic model describing the dynamics of_x000D_
aminoacids is combined with an empirical model to describe non-aminoacid components (salts, vitamins,_x000D_
metals, etc.). This model will be later used for media and process optimization., program purpose french : Chinese hamster ovary (CHO) cells are used extensively in the pharmaceutical industry to produce recombinant_x000D_
proteins to treat an array of diseases. The productivity and the therapeutic effectiveness of these proteins are_x000D_
highly influenced by the chemical composition of the chemical medium and the bioreactor operating conditions_x000D_
that are used to grow the cells._x000D_
Optimizing the media composition is very challenging due to its complex nature involving tens of different_x000D_
components such as aminoacids and non-aminoacid species (salts, vitamins, metals etc.)._x000D_
Our industrial collaborator (Sigma-Aldrich), a main manufacturer of cell culture media, is currently_x000D_
experimenting with an advanced system of 48 micro-reactors (Sartorius-AMBR 15, 15 ml volume each), that it_x000D_
is not available in our lab, to develop new cell lines and growth media. Although industrial antibody_x000D_
manufacturing involves either fed-batch or perfusion operations, the AMBR micro-bioreactor system is_x000D_
comparatively much easier, quicker and cheaper to run for the purpose of media development._x000D_
The work of my group in modeling of mammalian cells (Nikdel and Budman, 2016) can be directly applied to_x000D_
two aspects that are of interest for Sigma for the purpose of media development with the AMBR system:_x000D_
i- To expand the validity of the model developed at Waterloo under the first Engage project for describing both_x000D_
AMBR and perfusion operations using different media formulations and_x000D_
ii- To develop a novel hybrid modeling approach, where the metabolic model describing the dynamics of_x000D_
aminoacids is combined with an empirical model to describe non-aminoacid components (salts, vitamins,_x000D_
metals, etc.). This model will be later used for media and process optimization., agreement title english : Optimization of a Mammalian Cell Bioprocess using a Semi-Mechanistic Model, agreement title french : Optimization of a Mammalian Cell Bioprocess using a Semi-Mechanistic Model, agreement number : EGP2, agreement value : 12500.0, agreement start date : 2017-09-20, agreement end date : nan, description english : nan, description french : nan, expected results english : nan, expected results french : nan, additional information english : Grant or Award spanning more than one fiscal year (2017-2018 to 2018-2019)., additional information french : Subvention ou bourse octroyée s'appliquant à plus d'un exercice financier (2017-2018 à 2018-2019)., owner organization : nserc-crsng, owner organization title : Natural Sciences and Engineering Research Council of Canada | Conseil de recherches en sciences naturelles et en génie du Canada