Question: query about wilting problem in nursery. 
Answer: ooze test: ooze test is done to detect bacteria. in this the infected leaves are cut into small bits and put into water in a glass tube, the water becomes turbid and yellowish after the lapse of 30-60 minutes. this turbidity is due to the release of bacterial cells into the water from the cut ends of the leaf bits.
bacterial disease: spray streptocycline/streptomycin @ 1g/5 litre of water.
fungal disease: spray saaf/bavistin/ridomil gold @ 2ml or 2g per litre of water for 3 times at 5 days interval.