FrancophonIA/DGT-Translation_Memory huggingface dataset:
English: Hayes, B.B., Gerber, P.C., Griffey, S.S. and Meade, B.J. (1998), Contact hypersensitivity to dicyclohexylcarbodiimide and diisopropylcarbodiimide in female B6C3F1 mice, Drug. Chem.
Slovak: Hayes, B. B., Gerber, P. C., Griffey, S. S. and Meade, B. J. (1998), Contact hypersensitivity to dicyclohexylcarbodiimide and diisopropylcarbodiimide in female B6C3F1 mice, Drug.
English: Woolhiser, M.R., Hayes, B.B. and Meade, B.J. (1998), A combined murine local lymph node and irritancy assay to predict sensitisation and irritancy potential of chemicals, Toxicol.
Slovak: Woolhiser, M. R., Hayes, B. B. and Meade, B. J. (1998), A combined murine local lymph node and irritancy assay to predict sensitisation and irritancy potential of chemicals, Toxicol.
English: Hayes, B.B. and Meade, B.J. (1999), Contact sensitivity to selected acrylate compounds in B6C3F1 mice: relative potency, cross reactivity, and comparison of test methods, Drug. Chem.
Slovak: Hayes, B. B. and Meade, B. J. (1999), Contact sensitivity to selected acrylate compounds in B6C3F1 mice: relative potency, cross reactivity and comparison of test methods, Drug.
English: Vohr, H.W. and Ahr, H.J. (2005), The local lymph node assay being too sensitive?
Slovak: Vohr, H. W. and Ahr, H. J. (2005), The local lymph node assay being too sensitive?
English: The purpose of Performance Standards (PS) is to communicate the basis by which new test methods, both proprietary (i.e. copyrighted, trademarked, registered) and non-proprietary can be determined to have sufficient accuracy and reliability for specific testing purposes. These PS, based on validated and accepted test methods, can be used to evaluate the reliability and accuracy of other similar methods (colloquially referred to as “me-too” tests) that are based on similar scientific principles and measure or predict the same biological or toxic effect (14). 2. Prior to adoption of modified methods (i.e. proposed potential improvements to an approved test method), there should be an evaluation to determine the effect of the proposed changes on the test’s performance and the extent to which such changes affect the information available for the other components of the validation process.
Slovak: Cieľom noriem výkonnosti (PS) je oznámiť základ, na ktorom možno určiť, že nové testovacie metódy, či už chránené (t. j. chránené autorským právom, obchodnou značkou, registrované), alebo nechránené, sú dostatočne presné a spoľahlivé na konkrétne účely testovania.
English: Fentem, J.H., Briggs, D., Chesné, C., Elliot, G.R., Harbell, J.W., Heylings, J.R., Portes, P., Roguet, R., van de Sandt, J.J. M. and Botham, P. (2001), A prevalidation study on in vitro tests for acute skin irritation, Results and evaluation by the Management Team, Toxicol. in Vitro 15, 57-93.
Slovak: Fentem, J. H., Briggs, D., Chesné, C., Elliot, G. R., Harbell, J. W., Heylings, J. R., Portes, P., Roguet, R., van de Sandt, J. J. M. and Botham, P. (2001), A prevalidation study on in vitro tests for acute skin irritation, Results and evaluation by the Management Team, Toxicol. in Vitro 15, 57 – 93.
English: Harvell, J.D., Lamminstausta, K., and Maibach, H.I. (1995), Irritant contact dermatitis, In: Practical Contact Dermatitis, pp 7-18, (Ed. Guin J. D.).
Slovak: Harvell, J. D., Lamminstausta, K., and Maibach, H. I. (1995), Irritant contact dermatitis, In: Practical Contact Dermatitis, pp 7-18, (Ed. Guin J. D.).
English: Basketter, D.A., York, M., McFadden, J.P. and Robinson, M.K. (2004), Determination of skin irritation potential in the human 4-h patch test.
Slovak: Basketter, D. A., York, M., McFadden, J. P. and Robinson, M. K. (2004), Determination of skin irritation potential in the human 4-h patch test.
English: Fenech, M. and Morley, A.A. (1985), Solutions to the kinetic problem in the micronucleus assay, Cytobios., 43, 233-246.
Slovak: Fenech, M. and Morley, A. A. (1985), Solutions to the kinetic problem in the micronucleus assay, Cytobios., 43, 233 – 246.
English: Fenech, M. and Morley, A.A. (1986), Cytokinesis-block micronucleus method in human lymphocytes: effect of in-vivo ageing and low dose X-irradiation, Mutation Res., 161, 193-198.
Slovak: Fenech, M. and Morley, A. A. (1986), Cytokinesis-block micronucleus method in human lymphocytes: effect of in-vivo ageing and low dose X-irradiation, Mutation Res., 161, 193 – 198.
English: Eastmond, D.A. and Tucker, J.D. (1989), Identification of aneuploidy-inducing agents using cytokinesis-blocked human lymphocytes and an antikinetochore antibody, Environ.
Slovak: Eastmond, D. A. and Tucker, J. D. (1989), Identification of aneuploidy-inducing agents using cytokinesis-blocked human lymphocytes and an antikinetochore antibody, Environ.
English: Miller, B.M., Zitzelsberger, H.F., Weier, H.U. and Adler, I.D. (1991), Classification of micronuclei in murine erythrocytes: immunofluorescent staining using CREST antibodies compared to in situ hybridization with biotinylated gamma satellite DNA, Mutagenesis, 6, 297-302.
Slovak: Miller, B. M., Zitzelsberger, H. F., Weier, H. U. and Adler, I. D. (1991), Classification of micronuclei in murine erythrocytes: immunofluorescent staining using CREST antibodies compared to in situ hybridization with biotinylated gamma satellite DNA, Mutagenesi., 6, 297 – 302.
English: Farooqi, Z., Darroudi, F. and Natarajan, A.T. (1993), The use of fluorescence in-situ hybridisation for the detection of aneugens in cytokinesis-blocked mouse splenocytes, Mutagenesis, 8, 329-334.
Slovak: Farooqi, Z., Darroudi, F. and Natarajan, A. T. (1993), The use of fluorescence in-situ hybridisation for the detection of aneugens in cytokinesis-blocked mouse splenocytes, Mutagenesi., 8, 329 – 334.
English: Eastmond, D.A, Rupa, D.S. and Hasegawa, L.S. (1994), Detection of hyperdiploidy and chromosome breakage in interphase human lymphocytes following exposure to the benzene metabolite hydroquinone using multicolor fluorescence in situ hybridization with DNA probes, Mutation Res., 322, 9-20.
Slovak: Eastmond, D. A, Rupa, D. S. and Hasegawa, L. S. (1994), Detection of hyperdiploidy and chromosome breakage in interphase human lymphocytes following exposure to the benzene metabolite hydroquinone using multicolor fluorescence in situ hybridization with DNA probes, Mutation Res., 322, 9 – 20.
English: Marshall, R.R., Murphy, M., Kirkland, D.J. and Bentley, K.S. (1996), Fluorescence in situ hybridisation (FISH) with chromosome-specific centromeric probes: a sensitive method to detect aneuploidy, Mutation Res., 372, 233-245.
Slovak: Marshall, R. R., Murphy, M., Kirkland, D. J. and Bentley, K. S. (1996), Fluorescence in situ hybridisation (FISH) with chromosome-specific centromeric probes: a sensitive method to detect aneuploidy, Mutation Res., 372, 233 – 245.
English: Gibson, D.P., Brauninger, R., Shaffi, H.S., Kerckaert, G.A., LeBoeuf, R.A., Isfort, R.J. and Aardema, M.J. (1997), Induction of micronuclei in Syrian hamster embryo cells: comparison to results in the SHE cell transformation assay for National Toxicology Program test chemicals, Mutation Res., 392, 61-70.
Slovak: Gibson, D. P., Brauninger, R., Shaffi, H. S., Kerckaert, G. A., LeBoeuf, R. A., Isfort, R. J. and Aardema, M. J. (1997), Induction of micronuclei in Syrian hamster embryo cells: comparison to results in the SHE cell transformation assay for National Toxicology Program test chemicals, Mutation Res., 392, 61 – 70.
English: Scott, D., Galloway, S.M., Marshall, R.R., Ishidate, M. Jr., Brusick, D., Ashby, J. and Myhr, B.C. (1991), International Commission for Protection Against Environmental Mutagens and Carcinogens, Genotoxicity under extreme culture conditions.
Slovak: Scott, D., Galloway, S. M., Marshall, R. R., Ishidate, M. Jr., Brusick, D., Ashby, J. and Myhr, B. C. (1991), International Commission for Protection Against Environmental Mutagens and Carcinogens, Genotoxicity under extreme culture conditions.
English: Fenech, M. and Morley, A.A. (1985), Measurement of micronuclei in lymphocytes, Mutation Res., 147, 29-36.
Slovak: Fenech, M. and Morley, A. A. (1985), Measurement of micronuclei in lymphocytes, Mutation Res., 147, 29 – 36.
English: Maron, D.M. and Ames, B.N. (1983), Revised methods for the Salmonella mutagenicity test, Mutation Res., 113, 173-215.
Slovak: Maron, D. M. and Ames, B. N. (1983), Revised methods for the Salmonella mutagenicity test, Mutation Res., 113, 173 – 215.
English: Elliott, B.M., Combes, R.D., Elcombe, C.R., Gatehouse, D.G., Gibson, G.G., Mackay, J.M. and Wolf, R.C. (1992), Alternatives to Aroclor 1254-induced S9 in in-vitro genotoxicity assays.
Slovak: Elliott, B. M., Combes, R. D., Elcombe, C. R., Gatehouse, D. G., Gibson, G. G., Mackay, J. M. and Wolf, R. C. (1992), Alternatives to Aroclor 1254-induced S9 in in-vitro genotoxicity assays.
English: Matsushima, T., Sawamura, M., Hara, K. and Sugimura, T. (1976), A safe substitute for Polychlorinated Biphenyls as an Inducer of Metabolic Activation Systems, In: de Serres, F.J., Fouts, J. R., Bend, J.R. and Philpot, R.M. (eds), In Vitro Metabolic Activation in Mutagenesis Testing, Elsevier, North-Holland, pp. 85-88.
Slovak: Matsushima, T., Sawamura, M., Hara, K. and Sugimura, T. (1976), A safe substitute for Polychlorinated Biphenyls as an Inducer of Metabolic Activation Systems, In: de Serres, F. J., Fouts, J. R., Bend, J. R. and Philpot, R. M. (eds), In Vitro Metabolic Activation in Mutagenesis Testing, Elsevier, North-Holland, s. 85 – 88.
English: Johnson, T.E., Umbenhauer, D.R. and Galloway, S.M. (1996), Human liver S-9 metabolic activation: proficiency in cytogenetic assays and comparison with phenobarbital/beta-naphthoflavone or Aroclor 1254 induced rat S-9, Environ.
Slovak: Johnson, T. E., Umbenhauer, D. R. and Galloway, S. M. (1996), Human liver S-9 metabolic activation: proficiency in cytogenetic assays and comparison with phenobarbital/beta-naphthoflavone or Aroclor 1254 induced rat S-9, Environ.
English: Doherty, A.T., Ellard, S., Parry, E.M. and Parry, J.M. (1996), An investigation into the activation and deactivation of chlorinated hydrocarbons to genotoxins in metabolically competent human cells, Mutagenesis, 11, 247-274.
Slovak: Doherty, A. T., Ellard, S., Parry, E. M. and Parry, J. M. (1996), An investigation into the activation and deactivation of chlorinated hydrocarbons to genotoxins in metabolically competent human cells, Mutagenesi., 11, 247 – 274.
English: Krahn, D.F., Barsky, F.C. and McCooey, K.T. (1982), CHO/HGPRT Mutation Assay: Evaluation of Gases and Volatile Liquids, In: Tice, R.R., Costa, D.L. and Schaich, K.M. (eds), Genotoxic Effects of Airborne Agents.
Slovak: Krahn, D. F., Barsky, F. C. and McCooey, K. T. (1982), CHO/HGPRT Mutation Assay: Evaluation of Gases and Volatile Liquids, In: Tice, R. R., Costa, D. L. and Schaich, K. M. (eds), Genotoxic Effects of Airborne Agents.
English: Zamora, P.O., Benson, J.M., Li, A.P. and Brooks, A.L. (1983), Evaluation of an exposure system using cells grown on collagen gels for detecting highly volatile mutagens in the CHO/HGPRT mutation assay, Environ.
Slovak: Zamora, P. O., Benson, J. M., Li, A. P. and Brooks, A. L. (1983), Evaluation of an exposure system using cells grown on collagen gels for detecting highly volatile mutagens in the CHO/HGPRT mutation assay, Environ.
English: OECD (2010), Skin Sensitisation: Local Lymph Node Assay, Test Guideline No 429, Guidelines for the Testing of Chemicals, OECD, Paris. Available at: [http://www.oecd.org/env/testguidelines]
Slovak: Chamberlain, M. and Basketter, D. A. (1996), The local lymph node assay: status of validation.
English: Chamberlain, M. and Basketter, D.A. (1996), The local lymph node assay: status of validation.
Slovak: Food Chem, Toxicol., 34, 999 – 1002.
English: Basketter, D.A., Gerberick, G.F., Kimber, I. and Loveless, S.E. (1996), The local lymph node assay: A viable alternative to currently accepted skin sensitisation tests.
Slovak: Basketter, D. A., Gerberick, G. F., Kimber, I. and Loveless, S. E. (1996), The local lymph node assay: A viable alternative to currently accepted skin sensitisation tests.
English: Dexter, S.J., Cámara, M., Davies, M. and Shakesheff, K.M. (2003), Development of a bioluminescent ATP assay to quantify mammalian and bacterial cell number from a mixed population.
Slovak: Dexter, S. J., Cámara, M., Davies, M. and Shakesheff, K. M. (2003), Development of a bioluminescent ATP assay to quantify mammalian and bacterial cell number from a mixed population.
English: McGarry, H.F. (2007), The murine local lymph node assay: regulatory and potency considerations under REACH.
Slovak: McGarry, H. F. (2007), The murine local lymph node assay: regulatory and potency considerations under REACH.
English: Kimber, I., Dearman, R.J., Scholes E.W. and Basketter, D.A. (1994), The local lymph node assay: developments and applications.
Slovak: Kimber, I., Dearman, R. J., Scholes E. W. and Basketter, D. A. (1994), The local lymph node assay: developments and applications.
English: Hayes, B.B., Gerber, P.C., Griffey, S.S. and Meade, B.J. (1998), Contact hypersensitivity to dicyclohexylcarbodiimide and diisopropylcarbodiimide in female B6C3F1 mice. Drug.
Slovak: Hayes, B. B., Gerber, P. C., Griffey, S. S. and Meade, B. J. (1998), Contact hypersensitivity to dicyclohexylcarbodiimide and diisopropylcarbodiimide in female B6C3F1 mice.
English: Woolhiser, M.R., Hayes, B.B. and Meade, B.J. (1998), A combined murine local lymph node and irritancy assay to predict sensitisation and irritancy potential of chemicals.
Slovak: Woolhiser, M. R., Hayes, B. B., and Meade, B. J. (1998), A combined murine local lymph node and irritancy assay to predict sensitisation and irritancy potential of chemicals.
English: Hayes, B.B. and Meade, B.J. (1999), Contact sensitivity to selected acrylate compounds in B6C3F1 mice: relative potency, cross reactivity, and comparison of test methods. Drug Chem.
Slovak: Hayes, B. B., and Meade, B. J. (1999), Contact sensitivity to selected acrylate compounds in B6C3F1 mice: relative potency, cross reactivity, and comparison of test methods.
English: Kimber, I., Hilton, J., Dearman, R.J., Gerberick, G.F., Ryan, C.A., Basketter, D.A., Lea, L., House, R.V., Ladies, G.S., Loveless, S.E. and Hastings, K.L. (1998), Assessment of the skin sensitisation potential of topical medicaments using the local lymph node assay: An interlaboratory exercise.
Slovak: Kimber, I., Hilton, J., Dearman, R. J., Gerberick, G. F., Ryan, C. A., Basketter, D. A., Lea, L., House, R. V., Ladies, G. S., Loveless, S. E., and Hastings, K. L. (1998), Assessment of the skin sensitisation potential of topical medicaments using the local lymph node assay: An interlaboratory exercise.
English: From each mouse, a single-cell suspension of lymph node cells (LNC) excised bilaterally is prepared by gentle mechanical disaggregation through 200 micron-mesh stainless steel gauze or another acceptable technique for generating a single-cell suspension (e.g. use of a disposable plastic pestle to crush the lymph nodes followed by passage through a #70 nylon mesh). The procedure for preparing the LNC suspension is critical in this assay and therefore every operator should establish the skill in advance.
Slovak: Jednobunková suspenzia buniek lymfatických uzlín (LNC) odobraná bilaterálne z každej myši sa pripravuje jemným mechanickým pretlačením cez sitko z nerezovej ocele so šírkou oka 200 μm alebo inou prijateľnou technikou na prípravu jednobunkovej suspenzie (napr. pomocou plastového mažiara na jedno použitie, ktorým sa lymfatické uzliny rozdrvia a následne prepasírujú cez nylonové sitko #70.) Postup na prípravu suspenzie buniek lymfatických uzlín je v tejto skúške veľmi dôležitý, a preto by každý odborník mal byť v tomto postupe vopred vyškolený.
English: Chamberlain, M. and Basketter, D.A. (1996), The local lymph node assay: status of validation. Food Chem.
Slovak: Chamberlain, M. and Basketter, D. A. (1996), The local lymph node assay: status of validation.
English: Basketter, D.A., Gerberick, G.F., Kimber, I. and Loveless, S.E. (1996), The local lymph node assay: A viable alternative to currently accepted skin sensitisation tests. Food Chem.
Slovak: Basketter, D. A., Gerberick, G. F., Kimber, I. and Loveless, S. E. (1996), The local lymph node assay: A viable alternative to currently accepted skin sensitisation tests.
English: Hayes, B.B., Gerber, P.C., Griffey, S.S. and Meade, B.J. (1998), Contact hypersensitivity to dicyclohexylcarbodiimide and diisopropylcarbodiimide in female B6C3F1 mice. Drug Chem.
Slovak: Hayes, B. B., Gerber, P. C., Griffey, S. S., and Meade, B. J. (1998), Contact hypersensitivity to dicyclohexylcarbodiimide and diisopropylcarbodiimide in female B6C3F1 mice.
English: Hayes, B.B. and Meade, B.J. (1999), Contact sensitivity to selected acrylate compounds in B6C3F1 mice: relative potency, cross reactivity, and comparison of test methods. Drug.
Slovak: Hayes, B. B., and Meade, B. J. (1999), Contact sensitivity to selected acrylate compounds in B6C3F1 mice: relative potency, cross reactivity, and comparison of test methods.
English: Unlike guinea pig tests (i.e. B.6; OECD Test Guideline 406) (13) the LLNA does not require that challenge-induced dermal hypersensitivity reactions be elicited. Furthermore, the LLNA does not require the use of an adjuvant, as is the case for the guinea pig maximisation test (13). Thus, the LLNA reduces animal pain and distress. Despite the advantages of the LLNA over B.6 and OECD Test Guideline 406, it should be recognised that there are certain limitations that may necessitate the use of B.6 or OECD Test Guideline 406 (13) (e.g. false negative findings in the LLNA with certain metals, false positive findings with certain skin irritants (such as some surfactant type chemicals) (19) (20), or solubility of the test substance).
Slovenian: V nasprotju s preskusi na morskih prašičkih (tj. B.6; Smernica za preskušanje OECD (406) (13) LLNA ne zahteva, da se povzročijo izzvane reakcije prekomerne občutljivosti kože. Poleg tega LLNA ne zahteva uporabe pomožnega preskusa (13) kot pri maksimizacijskem preskusu na morskih prašičkih S tem LLNA zmanjšuje bolečino in trpljenje živali. Kljub prednostim LLNA v primerjavi z metodo B.6 in Smernico za preskušanje OECD 406 je treba upoštevati, da obstajajo nekatere omejitve, zaradi katerih je lahko nujna uporaba metode B.6 ali Smernice za preskušanje OECD 406 (13) (npr. lažni negativni izidi pri LLNA pri nekaterih kovinah, lažni pozitivni izidi pri nekaterih dražilnih snoveh za kožo [kot so nekatere vrste površinsko razgradljivih kemikalij] (19) (20), ali topnost preskusne snovi). Poleg tega je zaradi kemijskih razredov ali snovi s funkcionalnimi skupinami, za katere se je izkazalo, da lahko delujejo kot moteči dejavniki (21) lahko potrebna uporaba preskusov na morskih prašičkih (tj. B.6; Smernica za preskušanje OECD 406) (13). Na podlagi omejene podatkovne zbirke za validacijo, ki je obsegala zlasti pesticidne pripravke, je bolj verjetno, da bo za te vrste snovi pozitiven rezultat prinesla LLNA kot pa preskus na morskih prašičkih (22). Vendar pa bi se lahko pri preskušanju pripravkov preučila tudi možnost vključitve podobnih snovi z znanimi rezultati kot primerjalnih snovi, s čimer bi se dokazalo, da LLNA deluje pravilno (glej odstavek 16). Razen takšnih opredeljenih omejitev bi morala biti LLNA primerna za preskušanje katere koli snovi, razen če so s temi snovmi povezane lastnosti, ki lahko ovirajo natančnost LLNA.
English: In addition, chemical classes or substances containing functional groups shown to act as potential confounders (21) may necessitate the use of guinea pig tests (i.e. B.6; OECD Test Guideline 406) (13). Further, based on the limited validation database, which consisted primarily of pesticide formulations, the LLNA is more likely than the guinea pig test to yield a positive result for these types of test substances (22). However, when testing formulations, one could consider including similar substances with known results as benchmark substances to demonstrate that the LLNA is functioning properly (see paragraph 16). Other than such identified limitations, the LLNA should be applicable for testing any substances unless there are properties associated with these substances that may interfere with the accuracy of the LLNA. PRINCIPLE OF THE TEST
Slovenian: NAČELO PRESKUSA
English: Liquid chemicals may be applied neat or diluted prior to dosing. Insoluble chemicals, such as those generally seen in medical devices, should be subjected to an exaggerated extraction in an appropriate solvent to reveal all extractable constituents for testing prior to application to an ear of the mice.
Slovenian: Tekoče kemikalije se lahko nanesejo nerazredčene ali pa se razredčijo pred dajanjem odmerka Pri netopnih kemikalijah, kot so tiste, ki se običajno uporabljajo v medicinskih pripomočkih, je treba uporabiti močno povečano ekstrakcijo v ustreznem topilu, da se odkrijejo vse ekstraktibilne sestavine za preskušanje pred nanosom na uho miši.
English: The PC dose should be chosen such that it does not cause excessive skin irritation or systemic toxicity and the induction is reproducible but not excessive (i.e. a SI > 20 would be excessive). Preferred PC are 25 % hexyl cinnamic aldehyde (Chemical Abstracts Service (CAS) No 101-86-0) in acetone: olive oil (4:1, v/v) and 5 % mercaptobenzothiazole (CAS No 149-30-4) in N,N-dimethylformamide (see Appendix 1, Table 1).
Slovenian: Odmerek pozitivne kontrole je treba izbrati tako, da ne povzroči čezmernega draženja kože ali sistemske toksičnosti in da je indukcija ponovljiva, vendar ne čezmerna (tj. SI > 20 bi bil čezmeren) Priporočena pozitivna kontrola je 25-odstotni heksil cimetov aldehid (Služba za izmenjavo kemijskih izvlečkov [CAS] št. 101-86-0) v acetonu: olivno olje (4:1, v/v) in 5-odstotni merkaptobenzotiazol (št. CAS 149-30-4) v N,N-dimetilformamidu (glej Dodatek 1, preglednica 1).
English: Reference chemicals recommended for assessing performance [1] Category
Slovenian: Referenčne kemikalije, priporočene za ocenjevanje učinkovitosti [1]Kategorija
English: The PC dose should be chosen such that it does not cause excessive skin irritation or systemic toxicity and the induction is reproducible but not excessive (e.g. SI > 10 would be considered excessive). Preferred PC are 25 % hexyl cinnamic aldehyde (Chemical Abstracts Service (CAS) number 101-86-0) and 25 % eugenol (CAS number 97-53-0) in acetone: olive oil (4:1, v/v).
Slovenian: Odmerek pozitivne kontrole je treba izbrati tako, da ne povzroči čezmernega draženja kože ali sistemske toksičnosti in da je indukcija ponovljiva, vendar ne čezmerna (tj. SI > 10 bi se štel za čezmernega) Priporočena pozitivna kontrola je 25-odstotni heksil cimetov aldehid (Služba za izmenjavo kemijskih izvlečkov [CAS] št. 101-86-0) in 25-odstotni evgenol (št. CAS 97-53-0) v acetonu: olivnem olju (4:1, v/v).
English: Approximately 24 to 30 hours after the start of application on Day 7, humanely kill the animals. Excise the draining auricular lymph nodes from each mouse ear and process separately in phosphate buffered saline (PBS) for each animal.
Slovenian: Približno 24 do 30 ur po začetku nanašanja 7. dne se živali humano usmrti.
English: Day 6 Record the weight of each animal and any clinical observation. Approximately 24 hours (24 h) after BrdU injection, humanely kill the animals.
Slovenian: dan Zabeleži se masa vsake živali in morebitne klinične ugotovitve.
English: Intra-laboratory reproducibility A determination of the extent that qualified people within the same laboratory can successfully replicate results using a specific protocol at different times. Also referred to as within-laboratory reproducibility (14).
Swedish: Reproducerbarhet inom laboratoriet Ett mått på i vilken utsträckning kvalificerade personer inom samma laboratorium framgångsrikt kan upprepa resultat vid olika tidpunkter när de använder ett visst protokoll.