FrancophonIA/DGT-Translation_Memory huggingface dataset:
English: Sepedonicus, buffer controls used for extracting the bacterium and the DNA from the sample,
Dutch: controles met de buffer die bij de extractie van de bacterie en het DNA uit het monster is gebruikt;
English: Diagnosis is only completed if C. m. subsp. sepedonicus is isolated, subsequently identified (see section 9). and confirmed by a pathogenicity test (section 10). Although C. m. subsp. sepedonicus is a fastidious organism, it can be isolated from symptomatic tissue. However, it may be outgrown by rapidly growing saprophytic bacteria and, therefore, isolations directly from the tuber or stem tissue pellet (section 3.1.6 or 3.2.5) is difficult.
Dutch: De diagnose is pas compleet als C. m. ssp. sepedonicus geïsoleerd, geïdentificeerd (zie deel 9) en door middel van een pathogeniteitstest (deel 10) bevestigd is.
English: With selective medium and appropriate dilution of the resuspended pellet from the heel end cores or stems of potatoes direct isolation of C. m. subsp. sepedonicus may be possible. Isolations shall be made from all symptomatic potato tubers or stem segments and from eggplants were no symptoms are observed but IF/PCR test from the composite sample was positive (see section 7.10).
Dutch: Hoewel C. m. ssp. sepedonicus een moeilijk kweekbaar organisme is, kan het geïsoleerd worden uit weefsel dat de symptomen vertoont.
English: sepedonicus cells are killed at temperatures above 30 °C and the optimum temperature is 21 °C. To avoid contamination incubate positive control and negative control plants on clearly separated benches in a glasshouse or growth chamber or, in case space is limited, ensure strict separation between treatments.
Polish: Komórki C. m. sepedonicus giną w temperaturze powyżej 30° C, zaś temperatura dla nich optymalna wynosi 21° C. Aby uniknąć kontaminacji, należy inkubować rośliny stanowiące kontrolę pozytywną i negatywną w wyraźnie odizolowanych od siebie miejscach w szklarni lub w komorze wzrostowej lub, jeśli brak dostatecznie dużych powierzchni, zapewnić ścisłą izolację poszczególnych zabiegów.
English: Although isolation of the pathogen from plant material with typical symptoms by dilution plating is straightforward, culturing may fail from advanced stages of infection. Saprophytic bacteria which grow on diseased tissue may outgrow or inhibit the pathogen on the isolation medium.
Portuguese: Embora o isolamento por diluição em placas do patogéneo proveniente de material vegetal que apresente sintomas típicos seja simples, poderá não ser possível isolar a bactéria a partir de material vegetal em fase avançada de infecção devido à competição e/ou excessivo desenvolvimento de colónias de bactérias saprófitas.
English: Count the number of plants showing symptoms. C. m. subsp. sepedonicus causes leaf wilting in eggplants which may commence as a marginal or interveinal flaccidity.
Slovak: Vypočítajte počet rastlín, na ktorých s prejavujú symptóny C. m. subsp. sepedonicus, ktoré spôsobujú vädnutie listov na rastlinách baklažánu, ktoré sa môže začať buď okrajovým, alebo medzižilovým ochabnutím.
English: Incubate at 18 to 24 °C with sufficient light and high relative humidity with appropriate watering to avoid waterlogging or drought stress (section 7.7). With pure cultures, typical wilting should be obtained within two weeks but plants not showing symptoms (see section 7.8) after this time should be incubated up to three weeks at temperatures conducive to eggplant growth but not exceeding 25 °C (Appendix 8).
Slovak: Inkubujte pri teplote 18 – 24 °C pri dostatočnom svelte a relatívne vysokej vlhkosti s dostatočným zavlažovaním, aby sa predišlo strestu z podmokania alebo sucha (oddiel 7.7) U čistých kultúr by sa typické vädnutie malo získať do 2 týždňov, ale rastliny, ktoré nebudú po tomto období preukazovať symptómy (pozri 7.8), by sa mali inkubovať až do 3 týždňov pri teplotách prospievajúcich rastu baklažánov, ale nepresahujúcich teplotu 25 °C (dodatok 8).
English: If this is not possible, store them in the container, without addition of buffer, refrigerated for not longer than 72 hours or for not longer than 24 hours at room temperature. Drying and suberisation of cores, and growth of saprophytes during storage may hinder detection of the ring rot bacterium.
Slovenian: Če to ni mogoče, jih shranimo v posodi brez dodanega pufra v hladilniku za največ 72 ur ali na sobni temperaturi ne več kot 24 ur. Sušenje in suberizacija jeder ter rast saprofitov med shranjevanjem lahko ovira odkritje bakterije obročkaste gnilobe.
English: nutrient dextrose agar (for use in subculturing only), yeast peptone glucose agar,
Slovenian: hranilni agar z dekstrozo (samo za uporabo cepljenja kultur),
English: See section 3.2 for recommended sample sizes. (2)
Swedish: Metoder för extrahering och koncentrering av patogen beskrivs i avsnitt 3.2.
English: any remaining extract and additional prepared material for the screening test(s), e.g. immunofluorescence slides, and
Swedish: Allt kvarvarande extrakt och ytterligare förberett material för screeningtest(er), t.ex. immunofluorescensglas.
English: until the completion of the said methods. Retention of the tubers will enable variety testing to be undertaken where appropriate.
Swedish: Genom bevaring av knölarna kan vid behov sorttester göras.
English: a sample of the infected eggplant material inoculated with the tuber or plant extract, and
Swedish: Ett prov av det infekterade äggplantematerialet inokulerat med knöl- eller plantextrakt.
English: use as animal feed after heat treatment such that there is no risk of the organism surviving, or
Swedish: Användning som djurfoder efter värmebehandling så att det inte finns någon risk för att skadegöraren har överlevt.
English: disposal at an officially approved dedicated waste disposal site at which there is no identifiable risk of escape of the pathogen into the environment e.g. through seepage to agricultural land, or
Swedish: Bortskaffande på en officiellt godkänd specialiserad upplagsplats för avfall där det inte finns någon identifierbar risk för att patogenen sprids till omgivningen, t.ex. genom läckage till jordbruksmark.
English: industrial processing through direct and immediate delivery to a processing plant with officially approved waste disposal facilities for which it has been established that there is no identifiable risk of the organism spreading, and with a system of cleansing and disinfection of at least the departing vehicles, or
Swedish: Användning för industriell bearbetning genom direkt och omedelbar leverans till en bearbetningsanläggning med officiellt godkända anordningar för avfallshantering för vilken det har säkerställts att det inte finns någon identifierbar risk för att skadegöraren skall spridas, och med ett system för rengöring och desinfektion av åtminstone avgående fordon.
English: Potatoes intended for planting may only be handled at the same site, if this is done separately or after cleansing and disinfection, or
Swedish: Potatisar avsedda för sättning får behandlas på samma plats endast om detta sker separat eller efter rengöring och desinfektion.