FrancophonIA/DGT-Translation_Memory huggingface dataset:
English: The testing procedure is intended for detection of latent infections in potato tubers. A positive result from at least two screening tests, based on different biological principles, must be complemented by the isolation of the pathogen; followed by, in case of isolation of typical colonies, confirmation of a pure culture as C. m. subsp. sepedonicus.
Spanish: Este procedimiento de prueba se destina a la detección de infecciones latentes en tubérculos de patata mediante al menos dos pruebas de selección basadas en distintos principios biológicos que, de ser positivas, deberán complementarse con el aislamiento del patógeno; en caso de aislamiento de colonias típicas, se procede a continuación a la identificación de un cultivo puro como C. m. subsp. sepedonicus.
English: If the second or other tests are negative the sample is considered negative. Further tests are not necessary.
Spanish: Si la segunda prueba o el resto de las pruebas arrojan resultados negativos, la muestra se considerará negativa y no será necesario llevar a cabo más pruebas.
English: Use 500 µl to test for C. m. subsp. sepedonicus, 500 µl for Ralstonia solanacearum and 500 µl for reference purposes. Add sterile glycerol to final concentration of 10 to 25 % (v/v) to the 500 µl of the reference aliquot and to the remaining test aliquot, vortex and store at –16 to –24 °C (weeks) or at 68 to –86 °C (months).
Spanish: Utilizar 500 µl para la prueba de C. m. subsp. sepedonicus, 500 µl para Ralstonia solanacearum y 500 µl a efectos de referencia.
Italian: Usare 500 µl per il rilevamento di C. m. subsp. sepedonicus, 500 µl per il rilevamento di Ralstonia solanacearum e 500 µl a fini di riferimento.
English: The procedure can be conveniently applied for composite samples of up to 200 stem parts. (Where surveys are performed they should be based on a statistically representative sample of the plant population under investigation.)
French: La procédure peut facilement être appliquée à des échantillons composites comportant jusqu’à 200 parties de tiges (lorsque des enquêtes sont réalisées, elles doivent être fondées sur un échantillon statistiquement représentatif de la population végétale considérée.)
Spanish: El procedimiento se puede adaptar convenientemente para muestras mixtas de hasta 200 partes de tallos (los estudios deberán basarse en una muestra representativa desde el punto de vista estadístico de la población vegetal que se someta a investigación).
English: sample extract that previously tested negative for C. m. subsp.
Spanish: extracto de la muestra que previamente haya arrojado resultados negativos para C. m. subsp.
Italian: estratto del campione precedentemente risultato negativo per C. m. subsp.
Dutch: monsterextract waarbij een eerdere test op C. m. ssp. sepedonicus negatief was;
Swedish: Provextrakt som tidigare givit negativt svar när det testats med avseende på C. m. subsp.
Hungarian: Mintakivonat, amelyet előzőleg megvizsgáltak és a C. m. subsp.
Slovenian: Ekstrakt vzorca, ki je prej testiran z negativnim rezultatom na C. m. subsp.
Latvian: ekstrakta paraugs, kas pirms tam uzrādījis negatīvu rezultātu attiecībā uz C.m. subsp.
English: and a sample of the infected eggplant material inoculated with the tuber or plant extract,
Spanish: de una muestra de las berenjenas infectadas inoculadas con extracto de tubérculo o de planta,
Italian: un campione conservato del materiale di melanzana infetto inoculato con l’estratto di tubero o di pianta,
Portuguese: de uma amostra conservada de material de beringela infectado, inoculado com o extracto do tubérculo ou da planta,
English: disposal at an officially approved dedicated waste disposal site at which there is no identifiable risk of escape of the organism into the environment e.g. through seepage to agricultural land. The waste shall be conveyed directly to the site under containment conditions such that there is no risk of loss of the waste, or
Spanish: en un vertedero autorizado oficialmente a tal fin donde no se haya podido determinar riesgo alguno de escape del organismo al medio ambiente, por ejemplo, mediante la filtración a tierras de cultivo; los residuos se trasladarán directamente al vertedero en unas condiciones de confinamiento que impidan todo riesgo de pérdida de los mismos,
English: These solids shall be disposed of as set out in subparagraph (i). The liquid waste shall then be either:
Spanish: A continuación, los residuos líquidos se someterán a alguna de las medidas siguientes:
English: SEPEDONICUS (Spieckermann et Kotthoff) Davis et al.
French: SEPEDONICUS (Spieckermann et Kotthoff) Davis et al.
Italian: SEPEDONICUS (Spieckermann e Kotthoff) Davis et al.
Portuguese: SEPEDONICUS (Spieckermann e Kotthoff) Davis et al.
Finnish: -BAKTEERIN DIAGNOSOIMISEKSI, TOTEAMISEKSI JA TUNNISTAMISEKSI
Hungarian: DIAGNOSZTIZÁLÁSÁRA, KIMUTATÁSÁRA ÉS AZONOSÍTÁSÁRA
Estonian: SEPEDONICUS (Spieckermann et Kotthoff) Davis et al. DIAGNOOSIMISE, AVASTAMISE JA KINDLAKSMÄÄRAMISE ANALÜÜSIMETOODIKA
Latvian: DIAGNOSTIKAS, NOTEIKŠANAS UN IDENTIFIKĀCIJAS TESTĒŠANAS SHĒMA
Lithuanian: SEPEDONICUS (Spieckermann et Kotthoff) Davis et al., SUKELIANČIOS ŽIEDINĮ PUVINĮ, DIAGNOZAVIMO, NUSTATYMO IR IDENTIFIKAVIMO TYRIMO PLANAS
English: subsp. sepedonicus not detected sample not infected by C.m. subsp. sepedonicus
Estonian: subsp. sepedonicus
English: subsp. sepedonicus purify by subculture
Hungarian: AZONOSÍTÁSI VIZSGÁLAT (10)
Estonian: subsp. sepedonicus
English: The remaining sample solution (49 µl) can be stored at –20 °C after addition of 1 volume of 96 % ethanol. In case the FISH assay requires repeating, remove the ethanol by centrifugation and add an equal volume of 0,01M PB (mix by vortexing).
Estonian: Ülejäänud proovilahust (49 µl), millele on eelnevalt lisatud üks maht 96 % etanooli, võib säilitada –20 °C juures Juhul kui FISH-analüüsi tuleb uuesti teha, eemaldatakse etanool tsentrifuugimise teel ja lisatakse sellega võrdne kogus 0,01 M fosfaatpuhvrit (loksutatakse vorteksil).
English: SEPEDONICUS
French: SEPEDONICUS
Finnish: SEPEDONICUS -BAKTEERIN ERISTÄMINEN
Hungarian: SEPEDONICUS IZOLÁLÁSA
Estonian: SEPEDONICUS ISOLEERIMINE
Latvian: SEPEDONICUS IZOLĒŠANA
Lithuanian: SEPEDONICUS IŠSKYRIMAS
English: Determine the following phenotypic properties which are universally present or absent in C. m. subsp. sepedonicus, according to the methods of Lelliott and Stead (1987), Klement et al. (1990), Schaad (2001), Anonymous (1987).
French: Rechercher les caractéristiques phénotypiques ci-après qui sont systématiquement présentes ou absentes chez C. m. ssp. sepedonicus, suivant les méthodes de Lelliott et Stead (1987), de Klement et al. (1990), de Schaad (2001), anonyme (1987).
Italian: Determinare le seguenti proprietà fenotipiche, sistematicamente presenti o assenti in C. m. subsp. sepedonicus, secondo i metodi di Lelliott e Stead (1987), Klement et al. (1990), Schaad (2001), anonimo (1987).
Portuguese: Determinar as seguintes características fenotípicas que estão universalmente presentes ou ausentes em C. m. subsp. sepedonicus, de acordo com os métodos descritos por Lelliott e Stead (1987), Klement et al. (1990), Schaad (2001), Anónimo (1987).
Polish: Określić następujące właściwości fenotypowe, które powszechnie występują lub nie występują u C. m. subsp. sepedonicus, zgodnie z metodami opisanymi przez: Lelliott i Stead (1987), Klement et al. (1990), Schaad (2001), Anonymous (1987).
Slovenian: Opredelimo naslednje fenotipske lastnosti, ki so univerzalno prisotne ali odsotne v C. m. subsp. sepedonicus po metodah Lelliota in Steada (1987), Klementa in ostalih (1990), Schaada (2001), neznanega avtorja (1987).
Estonian: Määratakse järgmised fenotüübilised omadused, mis esinevad või puuduvad bakteril C. m. subsp. sepedonicus, kasutades järgmistes allikates kirjeldatud meetodeid: Lelliott ja Stead (1987), Klement et al. (1990), Schaad (2001), anonüümne (1987).
English: Variety testing may be undertaken to verify the identity of the contaminated and clonally related tubers or plants, and
Portuguese: O teste de variedade pode ser efectuado no sentido de verificar a identidade dos tubérculos ou das batateiras contaminados e com uma relação clonal,
Swedish: Sorttester kan göras för att styrka identiteten hos de knölar eller plantor som är smittade och som kommer ur kloner som är närbesläktade.
Estonian: Saastunud ja klooni kaudu seotud mugulate või taimede suguluse tõendamiseks võib teha sordikatseid,
English: 10 Figure 2.
Finnish: Työskentelylaimennos antiseerumista/vasta-aineesta
English: 1/100 empty empty  Decimal dilution of resuspended pellet
Finnish: Näyte 1
English: 1 2 3 4 5 Duplicate of sample 1 or sample 2
Finnish: Näytteen 1 toisinto tai näyte 2
English: the material specified in paragraph 1, and
Swedish: Det material som specificeras i punkt 1.
Finnish: 1 kohdassa tarkoitettu aineisto,
Slovak: materiály uvedené v odseku 1,
English: measures shall be taken to eliminate volunteer potato plants and other naturally found host plants of the organism, and
French: des mesures sont prises en vue d’éliminer les repousses de pommes de terre et les autres plantes hôtes de l’organisme spontanément présentes,
Finnish: toteutetaan toimenpiteitä itsestään leviävien perunakasvien ja muiden itsestään kylväytyvien kasvintuhoojan isäntäkasvien hävittämiseksi,
Lithuanian: yra imamasi priemonių, stengiantis išnaikinti savaime sudygstančius bulvių augalus ir kitus natūraliai aptiktus organizmo šeimininkus,
English: disposal at an officially approved dedicated waste disposal site at which there is no identifiable risk of escape of the organism into the environment e.g. through seepage to agricultural land. The waste shall be conveyed directly to the site under containment conditions such that there is no risk of loss of the waste,
French: dans un centre d’élimination des déchets officiellement agréé ne présentant aucun risque identifiable de propagation de l’organisme pathogène dans l’environnement par l'intermédiaire, par exemple, d’infiltrations dans des terres agricoles, les déchets étant acheminés directement vers le site dans des conditions d’isolement prévenant tout risque de perte,
Finnish: hävittämällä virallisesti hyväksytyllä tarkoitukseen varatulla jätteidenhävittämispaikalla, jossa ei ole tunnistettavaa riskiä kasvintuhoojan pääsystä ympäristöön, esimerkiksi maatalousmaahan imeytymisen kautta; jäte on kuljetettava suoraan hävittämispaikalle pakkaamalla se siten, ettei ole vaaraa jätteen häviämisestä,
English: Young infected leaves often continue to expand although, less so in the infected zones. This creates odd shaped leaves.
French: Les jeunes feuilles infectées continuent souvent à se développer, mais de façon moins marquée aux endroits infectés, ce qui donne aux feuilles des formes irrégulières.
Italian: Le giovani foglie infette spesso continuano a svilupparsi, sebbene in misura minore nelle zone colpite, e ciò le porta ad assumere forme inconsuete.
English: Wash the tubers. Use appropriate disinfectants (chlorine compounds when PCR-test is to be used in order to remove eventual pathogen DNA) and detergents between each sample.
French: Laver les tubercules en appliquant des désinfectants (composés chlorés lorsque le test PCR doit être utilisé pour éliminer l’ADN pathogène) et des détergents appropriés entre les échantillons.
English: It is imperative that all C. m. subsp. sepedonicus positive controls and samples are treated separately to avoid contamination. This applies to IF slides and to all tests.
French: Afin d’éviter toute contamination, il est impératif que tous les témoins positifs et échantillons de C. m. ssp. sepedonicus soient traités séparément, aussi bien pour les tests d’immunofluorescence que pour tous les autres tests.
English: Use validated antibodies (see web site http://forum.europa.eu.int/Public/irc/sanco/Home/main). The test should be performed on freshly-prepared sample extracts.
French: Utiliser des anticorps validés (voir site web à l’adresse: http://forum.europa.eu.int/Public/irc/sanco/Home/main).
English: 1/100 empty empty  Decimal dilution of resuspended pellet Sample 1 1 2 3 4 5 Duplicate of sample 1 or sample 2
French: Double de l’échantillon 1 ou de l’échantillon 2
English: Cover each sample well with approximately 50 µl of lysozyme solution and incubate for 10 minutes at room temperature. Then dip the slides in demineralised water, once only and dry with filter paper.
French: Couvrir intégralement chaque échantillon d’environ 50 µl de solution de lysozyme et laisser incuber 10 minutes à température ambiante, puis plonger une seule fois les lames dans de l’eau déminéralisée et les sécher au papier filtre.
English: Apply two coverslips (24 × 24 mm) to each slide without trapping air. Place the slides in the pre-warmed moist chamber and hybridise overnight in the oven at 55 °C in the dark.
French: Couvrir chaque lame de deux lamelles (24 × 24 mm) en ayant soin de ne pas emprisonner d’air, puis placer les lames dans la chambre d’hybridation humide préchauffée et les y laisser une nuit, dans l’obscurité et à une température de 55 °C, pour permettre le déroulement de l’hybridation.
English: In particular Erwinia chrysanthemi can cause leaf symptoms and wilt that is very similar to symptoms of C. m. sepedonicus. The only difference is blackening of the stems in case in Erwinia chrysanthemi infections.
French: Erwinia chrysanthemi, en particulier, peut induire au niveau des feuilles des symptômes et un flétrissement très semblables aux symptômes de C. m. sepedonicus, à cette seule différence près que les infections à Erwinia chrysanthemi provoquent un noircissement des tiges.
English: and a sample of the infected eggplant material inoculated with the tuber or plant extract, and
French: un échantillon d’aubergine infecté par l’inoculation d’extrait de tubercule ou de plante,
English: heated to a minimum of 60 °C throughout the entire volume during at least 30 minutes prior to disposal, or
French: soit chauffés à cœur à une température de 60 °C pendant au moins 30 minutes avant d’être éliminés,
Swedish: Upphettning av hela volymen till minst 60 °C under minst 30 minuter. Därefter bortskaffande.
English: subsp. sepedonicus NO YES sample infected by C.m. subsp. sepedonicus
Hungarian: subsp. sepedonicus-szal
English: PATHOGENICITY TEST (11) both tests confirm pure culture as C.m. subsp. sepedonicus
Hungarian: subsp. sepedonicus
English: Dilutions of resuspended pellet 1/100
Hungarian: Az újraszuszpendált üledék hígításai
English: Observe the slides immediately with a microscope fitted for epifluorescence microscopy at 630 or 1000x magnification under immersions oil. With a filter suitable for fluorescein isothiocyanate (FITC) eubacterial cells (including most gram negative cells) in the sample are stained fluorescent green.
Hungarian: Azonnal nézzük meg a lemezeket epifluoreszcens mikroszkóppal 630 nm hullámhosszon vagy 1000-szeres nagyításon olajimmerzió mellett.
English: Sepedonicus,
Swedish: Sepedonicus.
Hungarian: Sepedonicus-ra negatívnak bizonyult.
Slovenian: Sepedonicus.
Latvian: Sepedonicus klātbūtni,
English: Incubate plants in quarantine facilities for up to four weeks at 18 to 24 °C. Incubate plants with sufficient light and high humidity (70 to 80 %) and water to prevent water logging or wilting through water deficiency.
Italian: Mantenere le piante in incubazione in locali di quarantena fino a un massimo di 4 settimane a una temperatura compresa tra 18 e 24 °C, con condizioni di luce e acqua sufficienti e un’elevata umidità (70-80 %) per evitare un eccesso d’acqua o un avvizzimento dovuto a carenza d’acqua.
Polish: Rośliny inkubować w pomieszczeniach do kwarantanny w temperaturze 18–24 °C do 4 tygodni, przy dostatecznym oświetleniu i wysokiej wilgotności (70– 80 %) i podlewać je tak, aby nie dopuścić do gromadzenia się wody lub więdnięcia z powodu niedoboru wody.
Hungarian: Inkubáljuk a növényeket növény-egészségügyi zárlati létesítményekben 4 héten keresztül 18–24 °C-on, elegendő fény és magas relatív páratartalom (70–80 %) mellett, valamint a megfelelő öntözés biztosításával, hogy megelőzzük a vízzel való átitatódást, vagy a vízhiány okozta hervadást.
English: Member States shall adopt and publish, by 31 March 2007 at the latest, the laws, regulations and administrative provisions necessary to comply with this Directive. They shall forthwith communicate to the Commission the text of those provisions and a correlation table between those provisions and the Directive.
Italian: Gli Stati membri adottano e pubblicano entro il 31 marzo 2007 le disposizioni legislative, regolamentari ed amministrative necessarie per conformarsi alla presente direttiva e comunicano immediatamente alla Commissione il testo di tali disposizioni nonché una tabella di correlazione tra dette disposizioni e la direttiva.
English: a suspension of 106 cells per ml of C. m. subsp. sepedonicus in water from a virulent isolate (e.g. NCPPB 2140 or NCPPB 4053), if possible also use DNA extracted from positive control samples in the PCR test.
Italian: una sospensione di 106 cellule per ml di C. m. subsp. sepedonicus in acqua provenienti da un isolato virulento (per esempio NCPPB 2140 o NCPPB 4053),
English: Sample extracts should be as free as possible from soil. It could therefore in certain cases advisible to prepare extractions from washed potatoes if PCR protocols are to be used.
Italian: Per evitare potenziali contaminazioni, preparare i controlli positivi in un ambiente separato da quello dei campioni da saggiare.
English: DNA purification methods Use positive and negative control samples as described above.
Italian: Nei limiti del possibile, gli estratti del campione devono essere privi di residui di terra.
English: Wilts may also be induced by populations of other bacteria or fungi present in the tuber tissue pellet. These include Ralstonia solanacearum, Erwinia carotovora subsp. carotovora and E. carotovora subsp. atroseptica, Erwinia chrysanthemi, Phoma exigua var. foveata, as well as large populations of saprophytic bacteria.
Italian: L’avvizzimento delle foglie può anche essere provocato da popolazioni di altri batteri o funghi presenti nel precipitato di tessuto di tubero, fra i quali si annoverano Ralstonia solanacearum, Erwinia carotovora subsp. carotovora e E. carotovora subsp. atroseptica, Erwinia chrysanthemi, Phoma exigua var. foveata, nonché ampie popolazioni di batteri saprofiti.
English: as a result of the testing under Article 6, those tubers or plants with a sister or parental clonal relationship to the tubers or plants designated to be contaminated under Article 5(1)(a) and for which, although they may have tested negative for the organism, it appears that contamination is probable through a clonal link. Variety testing may be undertaken to verify the identity of the contaminated and clonally related tubers or plants, and
Italian: dopo le prove di cui all’articolo 6, tuberi o piante con una relazione clonale parentale o collaterale con tuberi o piante dichiarati contaminati ai sensi dell’articolo 5, paragrafo 1, lettera a), e per i quali, malgrado i risultati negativi nei saggi per l’individuazione dell’organismo nocivo, la contaminazione risulti probabile per legami di carattere clonale (per verificare l’identità di tuberi o piante contaminati ed aventi una tale relazione clonale può essere effettuata un’analisi della varietà),
English: use as ware potatoes intended for industrial processing, and intended for direct and immediate delivery to a processing plant with appropriate waste disposal facilities and a system of cleansing and disinfection of at least the departing vehicles, or
Italian: l’impiego come patate da consumo destinate alla trasformazione industriale e consegnate direttamente e immediatamente ad uno stabilimento dotato di strutture apposite per l’eliminazione dei rifiuti e di un dispositivo per la pulizia e la disinfezione almeno dei veicoli in uscita,
Swedish: Användning som industripotatis, och avsedd för direkt och omedelbar leverans till en bearbetningsanläggning som har lämpliga anordningar för avfallshantering och ett system för rengöring och desinfektion av åtminstone avgående fordon.
English: The waste shall be conveyed directly to the site under containment conditions such that there is no risk of loss of the waste, or
Italian: Il trasporto dei rifiuti in tale sito va effettuato servendosi di contenitori idonei ad eliminare ogni rischio di perdita dei rifiuti stessi;
Swedish: Avfallet skall föras direkt till platsen under så säkra omständigheter att det inte finns någon risk för att någon del av avfallet förloras.
English: incineration, or
Italian: incenerimento;
Swedish: Förbränning.
English: liquid waste: prior to disposal, liquid waste containing suspended solids shall be subjected to filtration or settlement processes to remove such solids. These solids shall be disposed of as set out in subparagraph (i).
Italian: rifiuti liquidi: prima dell’eliminazione i rifiuti liquidi che contengano materiale solido in sospensione vanno sottoposti a procedimenti di filtraggio o decantazione per rimuovere il materiale solido in questione, per la cui eliminazione si applicano le disposizioni di cui al precedente sottoparagrafo i).
English: If the second or other tests are negative the sample is considered negative. Further tests are not necessary. (4)
Lithuanian: Jei antrojo testo arba kitų testų rezultatai neigiami, bandinys laikomas neigiamu.
English: The selective isolation test and typical colony morphology are described in section 8. (5)
Lithuanian: Paskesnių testų atlikti nereikia.
English: Pipette a measured standard volume (15 µl is appropriate for 6 mm window diameter-scale up volume for larger windows) of the resuspended pellet and each dilution on a row of windows. The second row can be used as duplicate or for a second sample as presented in Figure 2.
Lithuanian: Standartinis pakartotinai suspenduotų ir kiekvieno praskiedimo nuosėdų tūris (15 µl tinka dirbti, kai naudojamos plokštelės, kurių langelio skersmuo yra 6 mm, o esant didesniems langeliams, padidinamas tūris) lašinamas į langelius vienoje langelių eilutėje.. Antrojoje eilutėje pakartojamas pirmasis bandinys arba lašinamas antrasis bandinys, kaip parodyta 2 pav.
English: The first well should have 1/2 of the titre (T/2), the others 1/4 of the titre (T/4), 1/2 of the titre (T/2), the titre (T) and twice the titre (2T). (ii) According to test slide preparation in 4.1(ii):
Lithuanian: Tiriamosios plokštelės paruošimas pagal 4.1(i) ir 4.3(i)
English: Prepare the working dilution (WD) of the antibody in IF buffer. The working dilution affects the specificity. Figure 1. Preparation of the test slide according to 4.1(i) and 4.3(i) Dilutions of resuspended pellet
Lithuanian: Pakartotinai suspenduotų nuosėdų praskiedimai
English: Arrange the slides on moist paper. Cover the test windows with the dilution of FITC conjugate used to determine the titre.
Lithuanian: Plokštelės išdėliojamos ant drėgno popieriaus, į langelius pripilama praskiesto titrui nustatyti naudojamo FITC konjugato.
English: Plants may outgrow the infection. Young eggplants are much more susceptible to low populations of C. m. subsp. sepedonicus than are older plants, hence the necessity to use plants at or just before leaf stage 3.
Lithuanian: Jauni baklažanai nedidelėms C. m. subsp. sepedonicus populiacijoms mažiau atsparūs nei senesni augalai, todėl būtina naudoti augalus, esančius trečioje lapelio stadijoje arba jos dar nepasiekusius.
English: As the infection progresses, destruction of the vascular tissue occurs; the outer cortex may become separated from the inner cortex. In advanced stages of infection, cracks appear on the surface of the tuber, which are often reddish-brown at their margins.
Latvian: Infekcijai izplatoties, notiek vadaudu sairšana; mizas un ārējo audu slānis var atdalīties no iekšējo audu slāņa Slimības vēlīnajās stadijās bumbuļu virsmā parādās plaisas, parasti ar sarkanīgi brūnas krāsas malām.
English: process immediately.
Latvian: apstrādāt nekavējoties.
English: Layout for FISH slide Sample 1 Blank Blank Blank Sample 2
Dutch: Overzicht van het FISH-glaasje
English: window 2 window 3 window 4 window 5
Dutch: Monster 2