FrancophonIA/DGT-Translation_Memory huggingface dataset:
English: Opina et al.
German: (1999); Opina et al.
Dutch: (1999), Opina e.a.
Swedish: [1999]; Opina m.fl.
Finnish: (1999); Opina et al.
Greek: (1999)· Opina et al.
Slovak: (1999); Opina a kol.
Hungarian: (1999); Opina et al.
Slovenian: (1999); Opina in sod.
Latvian: (1999), Opina et al.
Lithuanian: (1999); Opina et al.
English: (1997), Weller et al.
German: (1997), Weller et al.
Spanish: Weller et al.
Dutch: (1997), Weller e.a.
Swedish: [1997], Weller m.fl.
Slovak: (1997), Weller a kol.
Slovenian: (1997), Weller in sod.
Latvian: (1997), Weller et.al.
Lithuanian: (1997), Weller et al.
English: Isolate from symptomatic plants by removing a section of stem about 2 cm above the inoculation point. Comminute and suspend in a small volume of sterile distilled water or 50 mM phosphate buffer (Appendix 4).
German: Von Pflanzen mit Symptomen wie folgt isolieren: Ein Stängelstück 2 cm oberhalb der Inokulationsstelle herausschneiden, zerkleinern und in wenig sterilem destilliertem Wasser oder 50 mM Phosphatpuffer (Anlage 4) suspendieren.
English: CFBP 4608, Port 448, EURS80
French: CFBP 4608, Port 448, EURS80, NCPPB 4066
German: CFBP 4608, Port 448, EURS80, NCPPB 4066
Spanish: CFBP 4608, Port 448, EURS80, NCPPB 4066
Dutch: CFBP 4608, Port 448, EURS80, NCPPB 4066
Finnish: CFBP 4608, Port 448, EURS80, NCPPB 4066
Slovak: CFBP 4608, Port 448, EURS80, NCPPB 4066
Slovenian: CFBP 4608, Port 448, EURS80, NCPBB 4066
Estonian: CFBP 4608, Port 448, EURS80, NCPPB 4066
English: Commercially available standardised control material The following standard control material is available from the NCPPB culture collection.
German: Handelsübliches standardisiertes Kontrollmaterial
English: Transfer 100 µl of the suspension of R. solanacearum to the first microvial. Vortex.
German: 100 µl der Suspension von R. solanacearum in das erste Röhrchen geben und vortexen.
English: The six contaminated microvials will be used as positive controls. The four non-contaminated microvials will be used as negative controls.
German: Die sechs kontaminierten Röhrchen als Positivkontrollen, die vier nicht kontaminierten Röhrchen als Negativkontrollen verwenden.
English: NaH2PO4.2 H2O
French: NaH2PO4.2H2O
German: NaH2PO4.2H2O
Spanish: NaH2PO4.2H2O
Italian: NaH2PO4.2H2O
Dutch: NaH2PO4.2H2O
Swedish: NaH2PO4.2H2O
English: and
German: hierbei
Swedish: och
Greek: και
Polish: oraz
Estonian: ning
English: Glycerol (86 %)
French: Glycérol (86 %)
German: Glyzerin (86 %)
Spanish: Glicerol (86 %)
Italian: Glicerolo (86 %)
Portuguese: Glicerol (86 %)
Finnish: Glyseroli (86 %)
Greek: Γλυκερίνη (86 %)
Slovak: Glycerín (86 %)
Polish: Gliceryna (86 %)
Hungarian: Glicerin (86 %)
Slovenian: Glicerol (86 %)
Estonian: Glütserool (86 %)
Latvian: Glicerīns (86 %)
Lithuanian: Glicerinas (86 %)
English: 14 hours or natural day length if greater; Temperature
German: tagsüber: 21 bis 24 °C,
Spanish: diurna:21 a 24 °C
Greek: ημέρα: 21 έως 24 °C,
Polish: dzień: 21 do 24 °C,
Lithuanian: dienąnuo:21 iki 24 °C,
English: day 21 to 24 °C, night14 to 18 °C.
German: nachts: 14 bis 18 °C.
Spanish: nocturna:14 a 18 °C
Greek: νύχτα: 14 έως 18 °C.
Polish: noc: 14 do 18 °C.
Lithuanian: naktnuo: 14 iki 18 °C.
English: Disinfect stem segments briefly with ethanol 70 % and immediately blot dry on tissue paper. Then process the stem segments by one of the following procedures: either,
Greek: Τα τμήματα στελεχών απολυμαίνονται για σύντομο χρονικό διάστημα με αιθανόλη 70 % και στεγνώνονται αμέσως με απορροφητικό χαρτί.
English: Sample contaminated by R. solanacearum
Greek: Δείγμα προσβεβλημένο από το R. solanacearum
Estonian: Proov on nakatunud haigusetekitajaga R. solanacearum
English: Care is required to differentiate R. solanacearum from other bacteria able to develop colonies on the medium. Furthermore, colonies of R. solanacearum may show atypical morphology if plates are overcrowded or antagonistic bacteria are also present.
Greek: Επιπροσθέτως, οι αποικίες του R. solanacearum ενδέχεται να παρουσιάζουν μη τυπική μορφολογία εάν τα τρυβλία έχουν υπερβολικούς πληθυσμούς του βακτηρίου ή υπάρχουν επίσης ανταγωνιστικά βακτήρια.
English: Typical R. solanacearum colonies on SMSA medium are milky white, flat, irregular and fluidal and after three days incubation develop pink to blood-red coloration in the centre with internal streaking or whorling.
Finnish: Tyypilliset R. solanacearum -pesäkkeet SMSA-alustalla ovat maidonvalkoisia, litteitä, epäsäännöllisiä ja juoksevia, ja kolmen päivän inkubaation jälkeen niiden keskustaan kehittyy vaaleanpunaisesta verenpunaiseen vaihtelevaa väritystä, johon liittyy sisäinen raidoitus tai kierteet.
Greek: Οι τυπικές αποικίες του R. solanacearum σε θρεπτικό υπόστρωμα SMSA είναι γαλακτώδους χροιάς λευκές, επίπεδες, ακανόνιστες και ρευστώδεις και μετά από επώαση 3 ημερών αναπτύσσουν ρόδινο έως αιματέρυθρο χρώμα στο κέντρο με εσωτερικές ραβδώσεις ή ελικώσεις.
Polish: Typowe kolonie R. solanacearum na pożywce SMSA są mlecznobiałe, płaskie, nieregularne oraz ciekłe, a po 3 dniach inkubacji ich środek staje się od różowego do krwistoczerwonego z wewnętrznymi smugami lub spiralami.
Hungarian: A jellemző R. solanacearum telepek SMSA-táptalajon tejfehérek, laposak, szabálytalanok és elfolyósodottak, és 3 napos inkubáció után a közepükön a rózsaszíntől a vérvörösig terjedő elszíneződés alakul ki, melynek belsejében csíkok és spirálok mutatkoznak.
Estonian: Tüüpilised R. solanacearum’i kolooniad SMSA-söötmel on piimjasvalged, lamedad, ebaühtlase kujuga ja fluidaalsed ning pärast kolmepäevast inkubeerimist on nende värv keskel roosast veripunaseni, keskosas iseloomulike spiraalsete keerdudega.
Lithuanian: Tipiškos R. solanacearum kolonijos SMSA terpėje būna pieno baltumo, lygios, netaisyklingos formos bei takios ir po 3 inkubacijos dienų kolonijų centre atsiranda nuo rausvos iki kraujo spalvos centrinis vidinis ruožuotumas arba menturiai.
English: (see website http://forum.europa.eu.int/Public/irc/sanco/Home/main).
Finnish: (ks. www-sivusto osoitteessa http://forum.europa.eu.int/Public/irc/sanco/Home/main).
Greek: (βλ. http://forum.europa.eu.int/Public/irc/sanco/Home/main).
Polish: (Patrz: strona internetowa http://forum.europa.eu.int/Public/irc/sanco/Home/main).
Hungarian: (Lásd a honlapot: http://forum.europa.eu.int/Public/irc/sanco/Home/main).
Estonian: (vt veebileht http://forum.europa.eu.int/Public/irc/sanco/Home/main).
Lithuanian: (žr. svetainę http://forum.europa.eu.int/MembersPublic/irc/sanco/sanco_europhyt/libraryHome/main).
English: Test
Spanish: Pruebas
Italian: Saggio
Portuguese: Teste
Finnish: Testi
Greek: Δοκιμή
Hungarian: Vizsgálat
Estonian: Analüüs
Latvian: Tests
Lithuanian: Testas
English: O+/F–
Greek: O+/F-
Polish: O+/F—
English: Biovar
Finnish: Biovari
Greek: Βιοποικιλία
Polish: Biowar
Hungarian: Biovariáns
Estonian: Biotüüp
Latvian: Biovars
Lithuanian: Biovarietetas
English: Mannitol
Spanish: Manitol
Italian: Mannitolo
Portuguese: Manitol
Finnish: Mannitoli
Greek: Μαννιτόλης
Slovak: Manitu
Polish: Mannitolu
Slovenian: manitola
Estonian: mannitool
Latvian: mannīta
Lithuanian: Manitolį
English: Sorbitol
Italian: Sorbitolo
Finnish: Sorbitoli
Greek: Σορβιτόλης
Slovak: Sorbitu
Polish: Sorbitolu
Hungarian: Szorbitol
Slovenian: sorbitola
Estonian: sorbitool
Latvian: sorbīta
Lithuanian: Sorbitolį
English: Dulcitol
Italian: Dulcitolo
Finnish: Dulsitoli
Greek: Δουλκιτόλης
Slovak: Dulcitu
Polish: Dulcytolu
Slovenian: dulcitola
Estonian: dultsitool
Latvian: dulcīta
Lithuanian: Dulcitolį
English: Biovar 2A
Finnish: Biovari 2A
Greek: Βιοποικιλία 2Α
Hungarian: biovariáns
Estonian: Biotüüp 2A
Latvian: iovars
Lithuanian: Biovarietetas 2A
English: Biovar 2T
Finnish: Biovari 2T
Greek: Βιοποικιλία 2Τ
Hungarian: biovariáns
Estonian: Biotüüp 2T
Latvian: biovars
Lithuanian: Biovarietetas 2T
English: Edinburgh
French: Édimbourg
Spanish: Edimburgo
Italian: Edimburgo
Portuguese: Edimburgo
Greek: Εδιμβούργο
Polish: Edynburg
Slovenian: Edinburg
Latvian: Edinburga
Lithuanian: Edinburgas
English: Laboratoire National de la Protection des Végétaux, Unité de Bactériologie
Swedish: Laboratoire National de la Protection des Végétaux, Unité Bactériologie
Greek: Laboratoire National de la Protection des Végétaux, Unité Bactériologie
English: Hannover
French: Hanovre
Portuguese: Hanover
Greek: Αννόβερο
Latvian: Hannovere
Lithuanian: Hanoveris
English: Dublin
Spanish: Dublín
Greek: Δουβλίνο
Latvian: Dublina
Lithuanian: Dublinas
English: USA
French: États-Unis
Spanish: Estados Unidos
Portuguese: EUA
Finnish: Yhdysvallat
Greek: ΗΠΑ
Slovenian: ZDA
Latvian: ASV
Lithuanian: JAV
English: CFBP 1538
Spanish: CFBP 1538 Pseudomonas marginalis pv.
Greek: CFBP 1538 Pseudomonas marginalis pv.
English: Pseudomonas marginalis pv. marginalis [402]
Spanish: Marginalis [402]
Greek: Marginalis [402]
English: IVIA 1844.06
Portuguese: IVIA 1844,06
Dutch: IVIA 1844,06
Swedish: IVIA 1844,06
Greek: IVIA 1844,06
Slovenian: IVIA 1844,06
Latvian: IVIA 1844,06
English: Vortex. Establish decimal levels of contamination by further diluting in the next five microvials.
Greek: Προσδιορίζονται τα δεκαδικά επίπεδα μόλυνσης με περαιτέρω αραίωση στα επόμενα πέντε μικροφιαλιδία.
English: PCR protocol of Seal et al. (1993)
French: Protocole PCR de Seal et al. (1993)
Italian: Protocollo PCR di Seal et al. (1993)
Portuguese: Protocolo PCR de Seal et al. (1993)
Greek: Πρωτόκολλο PCR των Seal et al., (1993)
Polish: Protokół PCR Seal et al. (1993)
Estonian: PCR-protokoll, Seal et al. (1993)
English: The remaining sample solution (49 µl) can be stored at –20 °C after addition of one volume of 96 % ethanol. In case the FISH assay requires repeating, remove the ethanol by centrifugation and add an equal volume of 0,01 PB (mix by vortexing). Fig. 7.1 Layout for FISH slide
Spanish: La solución de la muestra restante (49 µl) puede almacenarse a –20 °C tras la adición de 1 volumen de etanol al 96 %.
English: Sample 1 Blank Blank Blank Sample 2
Spanish: En caso de que sea necesario repetir la prueba FISH, separar el etanol mediante centrifugación y añadir un volumen equivalente de tampón de fosfato de concentración 0,01 (mezclar mediante agitación).
English: window 1 window 2
Spanish: Figura 7.1.
English: window 3 window 4 window 5
Spanish: Distribución del portaobjetos para la prueba FISH.
English: Apply the IF procedure (Section VI.A.5.). 2.4. A positive IF test is achieved if the IF titre of the culture is equivalent to that of the positive control.
Spanish: Aplicar el procedimiento IF (sección VI.A.5).
English: Bacitracin (Sigma B-0125)
French: Bacitracine (Sigma B-0125)
Spanish: Bacitracina (Sigma B-0125)
Italian: Bacitracina (Sigma B-0125)
Portuguese: Bacitracina (Sigma B-0125)
Dutch: Bacitracine (Sigma B-0125)
Finnish: Basitrasiini (Sigma B-0125)
Slovak: Bacitracín (Sigma B-0125)
Polish: Bacytracyna (Sigma B-0125)
Estonian: Batsitratsiin (Sigma B-0125)
Latvian: Bacitracīns (Sigma B-0125)
Lithuanian: Bacitracino (Sigma B-0125)
English: IPO 1696a
Spanish: IPO 1 696a
English: Anti-foam agent [501] 1,0 ml Tetrasodium pyrophosphate Anti-oxidant
Spanish: Antiespumante [501]
Dutch: Antischuimmiddel [501]
Polish: Czynnik antypieniący [501]
Slovenian: protipenilno sredstvo [501]
English: Pellet buffer (10 mM phosphate buffer, pH 7,2) This buffer is used for resuspension and dilution of potato tuber heel-end core extracts following concentration to a pellet by centrifugation.
Spanish: Pirofosfato tetrasódico
English: Na2HPO4.12H2O 2,7 g NaH2PO4.2H2O
Spanish: Antioxidante
English: Na2HPO4.12H2O
Spanish: NaH2PO4.2H2O
Slovenian: KH2PO4
English: NaH2PO4.2H2O
Spanish: Agua destilada
Dutch: Na2HPO4.12H2O
Polish: Na2HPO4.12H2O
Slovenian: Na2HPO4.12H2O
English: NaCL
Spanish: Tampón IF [tampón fosfato salino (PBS) 10 mM, pH 7,2] Este tampón se utiliza para la dilución de anticuerpos. Na2HPO4.12H2O 2,7 g NaH2PO4.2H2O
Polish: NaH2PO4.2H2O
Slovenian: NaH2PO4.2H2O
English: IF-buffer-Tween This buffer is used to wash slides.
Spanish: Disolver los ingredientes, verificar el pH y esterilizar en autoclave a 121 °C durante 15 minutos.
English: Add 0,1 % Tween 20 to the IF buffer. 2.3. Phosphate buffered glycerol, pH 7,6
Spanish: Tampón IF-Tween
English: This buffer is used as a mountant fluid on the windows of IF slides to enhance fluorescence. Na2HPO4.12H2O 3,2 g NaH2PO4.2H2O
Spanish: Este tampón se utiliza para lavar los portaobjetos.
English: 10 % Tween 20
French: 10 % de Tween 20
Spanish: 10 % Tween 20
Portuguese: Tween 20 a 10 %
Slovak: 10 % tvín 20
English: 0,5 ml Milk powder
Spanish: 10 % Tween 20
Slovenian: 10 % Tween 20
English: Taq polymerase (5U/µl) [701] 0,1 µl
Spanish: Polimerasa Taq (5 U/µl) [701]
English: BSA (fraction V) (10 %) 0,25 µl
Spanish: BSA (fracción V) (10 %)
Slovenian: BSA (frakcija V) (10 %)
English: d-nTP mix (20 mM) 0,125 µl
Spanish: Mezcla d-nTP (20 mM)
English: Primer Ps-1 (10 µM) 0,5 µl
Spanish: Cebador Ps-1 (10 µM)
English: Primer NS-5-F (10 µM) [704] 0,15 µl
Spanish: Cebador NS-5-F (10 µM) [704]
Slovenian: Začetni oligonukleotid NS-5-F (10 µM) [704]
English: Taq polymerase (5 U/µl) [703] 0,2 µl
Spanish: Polimerasa Taq (5 U/µl) [703]
English: Taq polymerase (5U/µl) [705] 0,2 µl 1 U Sample volume
Spanish: Polimerasa Taq (5 U/µl) [705]
English: until the completion of the said methods. Retention of the tubers will enable variety testing to be undertaken where appropriate.
French: La conservation des tubercules permettra de tester les variétés, le cas échéant.
Spanish: La retención de los tubérculos permitirá que se puedan llevar a cabo pruebas de variedades cuando se estime oportuno.
Slovenian: Zadržanje gomoljev bo omogočilo testiranje sorte, kjer je to potrebno.
Lithuanian: Atskyrus stiebagumbius, tam tikrais atvejais bus galima ištirti įvairias veisles.
English: The elements to be considered in the determination of the extent of probable contamination under Article 5(1)(a)(iii) and 5(1)(c)(iii), shall include: the listed plant material grown at a place of production designated as contaminated under Article 5(1)(a)(ii),
French: La détermination de l’étendue de la contamination probable visée à l’article 5, paragraphe 1, point a) iii) et point c) iii), comprend les éléments suivants:
Spanish: Al determinar, con arreglo al artículo 5, apartado 1, letra a), inciso iii), y al artículo 5, apartado 1, letra c), inciso iii), el alcance de la contaminación probable, se incluirán los elementos siguientes:
English: in cases under Article 5(1)(a)(iv), the proximity of other places of production growing the listed plant material, the common production and use of seed potato stocks,
Spanish: Para la determinación de la posible propagación a la que se refieren el artículo 5, apartado 1, letra a), inciso iv), y el artículo 5, apartado 1, letra c), inciso iii), deberán tenerse en cuenta los elementos siguientes:
English: in cases where surface water has been designated as contaminated under Article 5(1)(c)(ii): place(s) of production producing listed plant material adjacent to, or at risk from flooding by, the surface water designated as contaminated, any discrete irrigation basin associated with the surface water designated as contaminated, water bodies connected with the surface water designated as contaminated, taking into account: the direction and rate of flow of the water designated as contaminated, the presence of wild solanaceous host plants.
Spanish: los lugares de producción en los que se utilicen aguas de superficie para el riego o rociamiento del material vegetal indicado cuando exista o haya existido el riesgo de escorrentía, o de anegamiento, de aguas superficiales procedentes de un lugar o lugares de producción que hayan sido declarados contaminados en virtud del artículo 5, apartado 1, letra a), inciso ii),
English: sample not contaminated by R. solanacearum
Estonian: Proov ei ole nakatunud haigusetekitajaga R. solanacearum
English: Sample not contaminated by R. solanacearum
Swedish: Koncentrering ökar populationerna av både patogener och konkurrerande saprofytiska bakterier och rekommenderas endast om den inte medför inhibering av isolationstestet.
Estonian: Proov ei ole nakatunud haigusetekitajaga R. solanacearum
English: Sample not contaminted by R. solanacearum
Estonian: Proov ei ole nakatunud haigusetekitajaga R. solanacearum
English: Validated polyclonal antisera all had an IF titre of at least 1:2000. During testing, the antibodies should be used at a working dilution(s) close to or at the titre.
Estonian: Analüüside käigus tuleks kasutada antikehade töölahuseid, mis on tiitri lähedal või sellega võrdsed.
English: Determine the following phenotypic properties, which are universally present or absent in R. solanacearum, according to the methods of Lelliott and Stead (1987), Klement et al. (1990), Schaad (2001).
French: Déterminer les caractéristiques phénotypiques suivantes, systématiquement présentes ou absentes chez R. solanacearum, suivant les méthodes de Lelliott et Stead (1987), Klement et al. (1990), et Schaad (2001).
Italian: Determinare le seguenti proprietà fenotipiche, sistematicamente presenti o assenti in R. solanacearum secondo i metodi di Lelliott e Stead (1987), Klement et al. (1990), Schaad (2001)
Portuguese: Determinar as seguintes características fenotípicas, que estão universalmente presentes ou ausentes em R. solanacearum, de acordo com os métodos de Lelliott e Stead (1987), Klement et al. (1990), Schaad (2001).
Polish: Określić następujące właściwości fenotypu, które standardowo występują lub nie w R. solanacearum, zgodnie z metodami Lelliotta i Steada (1987), Klementa et al. (1990), Schaad (2001).
Estonian: Määratakse järgmised fenotüübilised omadused, mis esinevad või puuduvad bakteril R. solanacearum, kasutades järgmistes allikates kirjeldatud meetodeid: Lelliott ja Stead (1987), Klement et al. (1990), Schaad (2001).
English: Apply appropriate PCR procedures to amplify R. solanacearum-specific amplicons (e.g. Seal et al. (1993); Pastrik and Maiss (2000); Pastrik et al. (2002); Boudazin et al. (1999); Opina et al. (1997), Weller et al. (1999).
French: Appliquer des procédures PCR appropriées pour amplifier les amplimères spécifiques de R. solanacearum [voir, par exemple: Seal et al. (1993), Pastrik & Maiss (2000), Pastrik et al. (2002), Boudazin et al. (1999), Opina et al. (1997), Weller et al. (1999)].
Portuguese: Aplicar os procedimentos PCR adequados para amplificar os fragmentos específicos de R. solanacearum [por exemplo Seal et al. (1993), Pastrik e Maiss (2000), Pastrik et al. (2002), Boudazin et al. (1999), Opina et al. (1997), Weller et al. (1999)].
Polish: Użyć właściwych procedur PCR dla namnożenia specyficznych dla R. solanacearum amplikonów (np. Seal et al. (1993); Pastrik i Maiss (2000); Pastrik et al. (2002); Boudazin et al. (1999); Opina et al. (1997), Weller et al. (1999).
Estonian: Bakteri R. solanacearum spetsiifiliste amplikonide amplifitseerimiseks tehakse kohane PCR-analüüs (nt Seal et al. (1993, Pastrik ja Maiss (2000), Pastrik et al. (2002), Boudazin et al. (1999), Opina et al. (1997), Weller et al. (1999)).
English: Hybmix
French: Hybmix 1X
Italian: 1 X Hybmix
Portuguese: “Hybmix” 1x
Swedish: 1 x Hybmix
Hungarian: Hibmix
Estonian: 1 X Hybmix-lahus
Latvian: hibridizācijas maisījums
Lithuanian: 1 kartą koncentruotas Hybmix
English: hybmix
French: Hybmix 3X
Portuguese: Hybmix 3x
Swedish: 3 x hybmix
Hungarian: hibmix
Estonian: 3 X Hybmix-lahus
Latvian: hibridizācijas maisījums
Lithuanian: hybmix tirpalas
English: Uppsala
Finnish: Upsala
Latvian: Upsala
Lithuanian: Upsala
English: MgSO4.7H2O
Finnish: Bacto-Agar (Difco)
Slovak: Bacto-agar (Difco)
Lithuanian: „Bacto“agaras (Difco)
English: Kelman’s Tetrazolium Medium Casamino acids (Difco)
Finnish: Bacto-Peptoni (Difco)
Slovak: Peptón bacto (Difco)
Lithuanian: „Bacto“ peptonas (Difco)
English: MgSO4
Finnish: NaNO3
Slovak: NaNO3
Lithuanian: NaNO3
English: SMT
Dutch: SMT-nr.
Swedish: SMT-nr
Finnish: SMT-nro
Slovenian: št. SMT
English: “‘Black Beauty’” Suppliers
Finnish: Tomaattien tai munakoisojen toimittajat
English: disposal at an officially approved dedicated waste disposal site at which there is no identifiable risk of escape of the organism into the environment e.g. through seepage to agricultural land or contact with water sources which could be used for irrigation of agricultural land. The waste shall be conveyed directly to the site under containment conditions such that there is no risk of loss of the waste,
Finnish: hävittämällä virallisesti hyväksytyllä tarkoitukseen varatulla jätteidenhävittämispaikalla, jossa ei ole tunnistettavaa riskiä kasvintuhoojan pääsystä ympäristöön, esimerkiksi siten, että se imeytyisi maatalousmaahan tai joutuisi kosketuksiin sellaisten vedensaantilähteiden kanssa, joita saatetaan käyttää maatalousmaan kasteluun; jäte on kuljetettava suoraan hävittämispaikalle pakkaamalla se siten, ettei ole vaaraa jätteen häviämisestä,
English: For description of symptoms see Section II.1. (2) Rapid diagnostic tests facilitate presumptive diagnosis but are not essential. A negative result does not always guarantee absence of the pathogen. (3) Streaming test for bacterial ooze from vascular stem tissue is described in Section VI.A.1.
French: Le test de purulence pour révéler l’exsudat bactérien des tissus vasculaires caulinaires est décrit dans la section VI.A.1.
English: Pathogen extraction and concentration methods are described in Section III.1.1. (3)
French: Si au moins deux tests fondés sur des principes biologiques différents sont positifs, l’isolement et la confirmation doivent être effectués.
English: Pathogen extraction and concentration methods are described in Section III.2.1. (3)
French: Si au moins deux tests fondés sur des principes biologiques différents sont positifs, l’isolement et la confirmation doivent être effectués.
English: Wash the tubers. Use appropriate disinfectants (chlorine compounds when PCR-test is to be used in order to remove pathogen DNA) and detergents between each sample.
French: Laver les tubercules en utilisant des désinfectants (composés chlorés lorsque le test PCR doit être utilisé pour éliminer l’ADN pathogène) et des détergents appropriés entre chaque échantillon.
English: It is imperative that all R. solanacearum positive controls and samples are treated separately to avoid contamination. This applies to IF slides and to all tests.
French: Il est impératif que tous les témoins positifs et les échantillons de R. solanacearum soient traités séparément afin d’éviter toute contamination, aussi bien pour les lames d’immunofluorescence que pour tous les tests.
English: Other hosts: With a clean disinfected knife or pruning shears, remove a 1 cm segment from the base of each stem, just above the soil level. In the case of S. dulcamara or other host plants growing in water, remove 1-2 cm sections from underwater stems or stolons with aquatic roots.
French: Dans le cas de S. dulcamara ou d’autres plantes hôtes poussant dans l’eau, enlever des morceaux de 1 à 2 cm des tiges se trouvant sous l’eau ou des stolons ayant des racines aquatiques.
English: Concentration of the pathogen (2) CORE SCREENING TEST Selective isolation (3)
French: Voir section IV.2.1 pour les procédures de prélèvement recommandées.
English: See Section IV.2.1. for recommended sampling procedures. (2)
French: Les méthodes de concentration de l’agent pathogène sont décrites dans la section IV.2.1.
English: Pathogen concentration methods are described in Section IV.2.1. Concentration increases populations of both pathogen and competing saprophytic bacteria and is recommended only if it does not result in inhibition of the isolation test.
French: La concentration augmente les populations de l’agent pathogène et des bactéries saprophytes concurrentes et n’est recommandée que si elle n’entraîne pas l’inhibition du test d’isolement.
Swedish: Metoder för anrikning plus PCR beskrivs i avsnitten VI.A.4.2 och VI.A.6.
English: selective isolation (2) optional auxiliary test: enrichment PCR (3) / bioassay (4) Colonies with typical morphology (5) after isolation All tests done negative NO (6) R. solanacearum not detected sample not contaminated by R. solanacearum YES Purify by subculture IDENTIFICATION TESTS (7) PATHOGENICITY TEST (8) Both tests confirm pure culture as R. solanacearum NO Sample not contaminted by R. solanacearum YES Sample contaminated by R. solanacearum (1)
French: Voir section V.2.1. pour les procédures de prélèvement recommandées.