FrancophonIA/DGT-Translation_Memory huggingface dataset:
English: Kristensen P. (1990). Evaluation of the Sensitivity of Short Term Fish Early Life Stage Tests in Relation to other FELS Test Methods. Final report to the Commission of the European Communities, 60 pp. June 1990.
Finnish: Kristensen P. (1990). Evaluation of the Sensitivity of Short Term Fish Early Life Stage Tests in Relation to other FELS Test Methods. Final report to the Commission of the European Communities, 60 pp. June 1990.
English: ASTM (1988). Standard Guide for Conducting Early Life-Stage Toxicity Tests with Fishes. American Society for Testing and Materials. E 1241-88. 26 pp.
Finnish: ASTM (1988). Standard Guide for Conducting Early Life-Stage Toxicity Tests with Fishes. American Society for Testing and Materials. E 1241-88. 26 pp.
English: Brauhn J. L. and Schoettger R. A. (1975). Acquisition and Culture of Research Fish: Rainbow trout, Fathead minnows, Channel Catfish and Bluegills. p. 54, Ecological Research Series, EPA-660/3-75-011, Duluth, Minnesota.
Finnish: Brauhn J. L. and Schoettger R. A. (1975). Acquisition and Culture of Research Fish: Rainbow trout, Fathead minnows, Channel Catfish and Bluegills. p. 54, Ecological Research Series, EPA-660/3-75-011, Duluth, Minnesota.
English: Brungs W. A. and Jones B. R. (1977). Temperature Criteria for Freshwater Fish: Protocol and Procedures. p. 128, Ecological Research Series EPA-600/3-77-061, Duluth, Minnesota.
Spanish: Brungs W.A. y Jones B.R. (1977) Temperature Criteria for Freshwater Fish: Protocol and Procedures p. 128, Ecological Research Series EPA-600/3-77-061, Duluth, Minnesota.
Finnish: Brungs W. A. and Jones B. R. (1977). Temperature Criteria for Freshwater Fish: Protocol and Procedures. p. 128, Ecological Research Series EPA-600/3-77-061, Duluth, Minnesota.
English: Laale H. W. (1977). The Biology and Use of the Zebrafish (Brachydanio rerio) in Fisheries Research. A Literature Review. J. Biol. 10, pp. 121-173.
Finnish: Laale H. W. (1977). The Biology and Use of the Zebrafish (Brachydanio rerio) in Fisheries Research. A Literature Review. J. Biol. 10, pp. 121-173.
English: Legault R. (1958). A Technique for Controlling the Time of Daily Spawning and Collecting Eggs of the Zebrafish, Brachydanio rerio (Hamilton-Buchanan) Copeia, 4, pp. 328-330.
Spanish: Legault R. (1958) A Technique for Controlling the Time of Daily Spawning and Collecting Eggs of the Zebrafish, Brachydanio rerio (Hamilton-Buchanan) Copeia, 4, pp 328-330.
Finnish: Legault R. (1958). A Technique for Controlling the Time of Daily Spawning and Collecting Eggs of the Zebrafish, Brachydanio rerio (Hamilton-Buchanan) Copeia, 4, pp. 328-330.
English: Dave G., Damgaard B., Grande M., Martelin J. E., Rosander B. and Viktor T. (1987). Ring Test of an Embryo-larval Toxicity Test with Zebrafish (Brachydanio rerio) Using Chromium and Zinc as Toxicants. Environmental Toxicology and Chemistry, 6, pp. 61-71.
Finnish: Dave G., Damgaard B., Grande M., Martelin J. E., Rosander B. and Viktor T. (1987). Ring Test of an Embryo-larval Toxicity Test with Zebrafish (Brachydanio rerio) Using Chromium and Zinc as Toxicants. Environmental Toxicology and Chemistry, 6, pp. 61-71.
English: Birge J. W., Black J. A. and Westerman A. G. (1985). Short-term Fish and Amphibian Embryo-larval Tests for Determining the Effects of Toxicant Stress on Early Life Stages and Estimating Chronic Values for Single Compounds and Complex Effluents. Environmental Toxicology and Chemistry 4, pp. 807-821.
Finnish: Birge J. W., Black J. A. and Westerman A. G. (1985). Short-term Fish and Amphibian Embryo-larval Tests for Determining the Effects of Toxicant Stress on Early Life Stages and Estimating Chronic Values for Single Compounds and Complex Effluents. Environmental Toxicology and Chemistry 4, pp. 807-821.
English: Van Leeuwen C. J., Espeldoorn A. and Mol F. (1986). Aquatic Toxicological Aspects of Dithiocarbamates and Related Compounds. Embryolarval Studies with Rainbow Trout (Salmo gairdneri). J. Aquatic Toxicology, 9, pp. 129-145.
Finnish: Van Leeuwen C. J., Espeldoorn A. and Mol F. (1986). Aquatic Toxicological Aspects of Dithiocarbamates and Related Compounds. Embryolarval Studies with Rainbow Trout (Salmo gairdneri). J. Aquatic Toxicology, 9, pp. 129-145.
English: Kirchen R. V. and W. R. West (1969). Teleostean Development. Carolina Tips 32(4): 1-4. Carolina Biological Supply Company.
Finnish: Kirchen R. V. and W. R. West (1969). Teleostean Development. Carolina Tips 32(4): 1-4. Carolina Biological Supply Company.
English: Kirchen R. V. and W. R. West (1976). The Japanese Medaka. Its care and Development. Carolina Biological Supply Company, North Carolina. 36 pp.
Finnish: Kirchen R. V. and W. R. West (1976). The Japanese Medaka. Its care and Development. Carolina Biological Supply Company, North Carolina. 36 pp.
English: Dunnett C. W. (1955). A Multiple Comparisons Procedure for Comparing Several Treatments with Control. J. Amer. Statist. Assoc., 50, pp. 1096-1121.
Finnish: Dunnett C. W. (1955). A Multiple Comparisons Procedure for Comparing Several Treatments with Control. J. Amer. Statist. Assoc., 50, pp. 1096-1121.
English: Mc Clave J. T., Sullivan J. H. and Pearson J.G. (1980). Statistical Analysis of Fish Chronic Toxicity Test Data. Proceedings of 4th Aquatic Toxicology Symposium, ASTM, Philadelphia.
Finnish: Mc Clave J. T., Sullivan J. H. and Pearson J.G. (1980). Statistical Analysis of Fish Chronic Toxicity Test Data. Proceedings of 4th Aquatic Toxicology Symposium, ASTM, Philadelphia.
English: Van Leeuwen C. J., Adema D. M. M. and Hermes J. (1990). Quantitative Structure-Activity Relationships for Fish Early Life Stage Toxicity. Aquatic Toxicology, 16, pp. 321-334.
Finnish: Van Leeuwen C. J., Adema D. M. M. and Hermes J. (1990). Quantitative Structure-Activity Relationships for Fish Early Life Stage Toxicity. Aquatic Toxicology, 16, pp. 321-334.
English: Environment Canada. (1992). Toxicity Tests Using Early Life Stages of Salmonid Fish (Rainbow Trout, Coho Salmon or Atlantic Salmon). Biological Test Method Series. Report EPS 1/RM/28, December 1992, 81 pp.
Finnish: Environment Canada. (1992). Toxicity Tests Using Early Life Stages of Salmonid Fish (Rainbow Trout, Coho Salmon or Atlantic Salmon). Biological Test Method Series. Report EPS 1/RM/28, December 1992, 81 pp.
English: Dave G. and Xiu R. (1991). Toxicity of Mercury, Nickel, Lead and Cobalt to Embryos and Larvae of Zebrafish, Brachydanio rerio. Arch. of Environmental Contamination and Toxicology, 21, pp. 126-134.
Finnish: Dave G. and Xiu R. (1991). Toxicity of Mercury, Nickel, Lead and Cobalt to Embryos and Larvae of Zebrafish, Brachydanio rerio. Arch. of Environmental Contamination and Toxicology, 21, pp. 126-134.
English: Meyer A., Bierman C. H. and Orti G. (1993). The phylogenetic position of the Zebrafish (Danio rerio), a model system in developmental biology - an invitation to the comperative methods. Proc. Royal Society of London, Series B, 252: pp. 231-236.
Finnish: Meyer A., Bierman C. H. and Orti G. (1993). The phylogenetic position of the Zebrafish (Danio rerio), a model system in developmental biology - an invitation to the comperative methods. Proc. Royal Society of London, Series B, 252: pp. 231-236.
English: Ghillebaert F., Chaillou C., Deschamps F. and Roubaud P. (1995). Toxic Effects, at Three pH Levels, of Two Reference Molecules on Common Carp Embryo. Ecotoxicology and Environmental Safety 32, pp. 19-28.
Finnish: Ghillebaert F., Chaillou C., Deschamps F. and Roubaud P. (1995). Toxic Effects, at Three pH Levels, of Two Reference Molecules on Common Carp Embryo. Ecotoxicology and Environmental Safety 32, pp. 19-28.
English: US EPA, (1991). Guidelines for Culturing the Japanese Medaka, Oryzias latipes. EPA report EPA/600/3-91/064, Dec. 1991, EPA, Duluth.
Finnish: US EPA, (1991). Guidelines for Culturing the Japanese Medaka, Oryzias latipes. EPA report EPA/600/3-91/064, Dec. 1991, EPA, Duluth.
English: US EPA, (1991). Guidelines for Conducting Early Life Stage Toxicity Tests with Japanese Medaka, (Oryzias latipes). EPA report EPA/600/3-91/063, Dec. 1991, EPA, Duluth.
Finnish: US EPA, (1991). Guidelines for Conducting Early Life Stage Toxicity Tests with Japanese Medaka, (Oryzias latipes). EPA report EPA/600/3-91/063, Dec. 1991, EPA, Duluth.
English: De Graeve G. M., Cooney J. D., McIntyre D. O., Poccocic T. L., Reichenbach N. G., Dean J. H. and Marcus M. D. (1991). Validity in the performance of the seven-day Fathead minnow (Pimephales promelas) larval survival and growth test: an intra- and interlaboratory study. Environ. Tox. Chem. 10, pp. 1189-1203.
Finnish: De Graeve G. M., Cooney J. D., McIntyre D. O., Poccocic T. L., Reichenbach N. G., Dean J. H. and Marcus M. D. (1991). Validity in the performance of the seven-day Fathead minnow (Pimephales promelas) larval survival and growth test: an intra- and interlaboratory study. Environ. Tox. Chem. 10, pp. 1189-1203.
English: Calow P. (1993). Handbook of Ecotoxicology, Blackwells, Oxford. Vol. 1, Chapter 10: Methods for spawning, culturing and conducting toxicity tests with Early Life stages of Estuarine and Marine fish.
Finnish: Calow P. (1993). Handbook of Ecotoxicology, Blackwells, Oxford. Vol. 1, Chapter 10: Methods for spawning, culturing and conducting toxicity tests with Early Life stages of Estuarine and Marine fish.
English: Balon E. K. (1985). Early life history of fishes: New developmental, ecological and evolutionary perspectives, Junk Publ., Dordrecht, 280 pp.
Finnish: Balon E. K. (1985). Early life history of fishes: New developmental, ecological and evolutionary perspectives, Junk Publ., Dordrecht, 280 pp.
English: Blaxter J. H. S. (1988). Pattern and variety in development, in: W. S. Hoar and D. J. Randall eds., Fish Physiology, Vol. XIA, Academic press, pp. 1-58.
Finnish: Blaxter J. H. S. (1988). Pattern and variety in development, in: W. S. Hoar and D. J. Randall eds., Fish Physiology, Vol. XIA, Academic press, pp. 1-58.
English: A spawning tray is placed at the bottom of the aquarium in the afternoon the day before start of the test. The spawning tray consists of a frame (plexi-glass or other suitable material), 5-7 cm high with a 2-5 mm coarse net attached at the top and a 10-30 μm fine net at the bottom.
Finnish: Testiä edeltävänä päivänä iltapäivällä akvaarion pohjalle asetetaan parittelutarjotin, jossa on 5-7 cm korkea kehys (pleksilasia tai muuta sopivaa materiaalia), 2-5 mm:n isoreikäinen verkko yläosassa ja 10-30 μm:n hieno verkko pohjassa.
English: After the fish have been left in dark for 12 hours, a faint light is turned on which will initiate the spawning. Two to four hours after spawning, the spawning tray is removed and the eggs collected.
Finnish: Kun kalat ovat olleet pimeässä 12 tuntia, sytytetään heikko valo, joka käynnistää parittelun. Kahdesta neljään tuntia parittelun jälkeen tarjotin otetaan pois ja munat kerätään.
English: Five to 10 male and female fish are housed individually at least two weeks prior to the intended spawning. After 5-10 days, the abdomens of the females will be distended and their genital papillae visible.
Finnish: Viidestä kymmeneen koiras- ja naaraskalaa kasvatetaan yksittäin vähintään 2 viikkoa ennen aiottua parittelua. 5-10 päivän kuluttua naaraiden vatsat ovat isot ja genitaalinystyt näkyviä.
English: Male fish lack papillae. Spawning is performed in spawning tanks equipped with a false mesh bottom (as above).
Finnish: Koiraskaloilla ei ole kyseisiä nystyjä. Parittelu tapahtuu parittelutankeissa, joissa on verkosta tehty valepohja (kuten edellä).
English: CALCULATIONS AND STATISTICS A two-stage approach is proposed.
Finnish: LASKUTOIMITUKSET JA TILASTOLLINEN KÄSITTELY Suositellaan, että tulosten käsittelyssä on kaksi vaihetta.
English: Laale H. W. (1977). The Biology and Use of the Zebrafish (Brachydanio rerio) in Fisheries Research. A Literature Review. J. Fish Biol. 10, pp. 121-173.
Finnish: Laale H. W. (1977). The Biology and Use of the Zebrafish (Brachydanio rerio) in Fisheries Research. A Literature Review. J. Fish Biol. 10, pp. 121-173.
English: Hisaoka K. K. and Battle H. I. (1958). The Normal Development Stages of the Zebrafish Brachydanio rerio (Hamilton-Buchanan) J. Morph., 102, 311 pp.
Finnish: Hisaoka K. K. and Battle H. I. (1958). The Normal Development Stages of the Zebrafish Brachydanio rerio (Hamilton-Buchanan) J. Morph., 102, 311 pp.
English: Untersuchungen zur Eiproduktion beim Zebrabärbling (Brachydanio rerio Hamilton-Buchanan). Journal of Applied Ichthyology, 2, pp. 173-181.
Finnish: Untersuchungen zur Eiproduktion beim Zebrabärbling (Brachydanio rerio Hamilton-Buchanan). Journal of Applied Ichthyology, 2, pp. 173-181.
English: Finney D. J. (1971). Probit Analysis, 3rd ed., Cambridge University Press, Great Britain, pp. 1-333.
Portuguese: Finney D. J. (1971). Probit Analysis, 3rd ed., Cambridge University Press, Great Britain, pp. 1-333.
Finnish: Finney D. J. (1971). Probit Analysis, 3rd ed., Cambridge University Press, Great Britain, pp. 1-333. (5) Stephan C. E. (1982).
English: Stephan C. E. (1982). Increasing the Usefulness of Acute Toxicity Tests. Aquatic Toxicology and Hazard Assessment: Fifth Conference, ASTM STP 766, J. G. Pearson, R. B. Foster and W. E. Bishop, Eds., American Society for Testing and Materials, pp. 69-81.
Finnish: Increasing the Usefulness of Acute Toxicity Tests. Aquatic Toxicology and Hazard Assessment: Fifth Conference, ASTM STP 766, J. G. Pearson, R. B. Foster and W. E. Bishop, Eds., American Society for Testing and Materials, pp. 69-81.
English: Dunnett C. W. (1955). A Multiple Comparisons Procedure for Comparing Several Treatments with a Control. J. Amer. Statist. Assoc., 50, pp. 1096-1121.
Finnish: Dunnett C. W. (1955). A Multiple Comparisons Procedure for Comparing Several Treatments with a Control. J. Amer. Statist. Assoc., 50, pp. 1096-1121.
English: Dunnett C. W. (1964). New Tables for Multiple Comparisons with a Control. Biometrics, 20, pp. 482-491.
Finnish: Dunnett C. W. (1964). New Tables for Multiple Comparisons with a Control. Biometrics, 20, pp. 482-491.
English: Williams D. A. (1971). A Test for Differences Between Treatment Means when Several Dose Levels are Compared with a Zero Dose Control. Biometrics, 27, pp. 103-117.
Finnish: Williams D. A. (1971). A Test for Differences Between Treatment Means when Several Dose Levels are Compared with a Zero Dose Control. Biometrics, 27, pp. 103-117.
English: Williams D. A. (1972). The Comparison of Several Dose Levels with a Zero Dose Control. Biometrics 28, pp. 519-531.
Finnish: Williams D. A. (1972). The Comparison of Several Dose Levels with a Zero Dose Control. Biometrics 28, pp. 519-531.
English: Sokal R. R. and Rohlf F. J. (1981). Biometry, the Principles and Practice of Statistics in Biological Research, W. H. Freeman and Co., San Francisco.APPENDIX 2
Finnish: Sokal R. R. and Rohlf F. J. (1981). Biometry, the Principles and Practice of Statistics in Biological Research, W. H. Freeman and Co., San Francisco.LISÄYS 2
English: INTRODUCTION This toxicity test is a laboratory method, designed to assess the oral acute toxicity of plant protection products and other chemicals, to adult worker honeybees.
Finnish: JOHDANTO Tämä myrkyllisyystesti on laboratoriomenetelmä, jonka tarkoituksena on arvioida kasvinsuojeluaineiden ja muiden kemikaalien välitöntä myrkyllisyyttä täysikasvuisille työmehiläisille oraalisen eli suun kautta tapahtuneen altistuksen jälkeen.
English: DEFINITIONS Acute oral toxicity:
Finnish: MÄÄRITELMÄT Kosketusaltistuksen jälkeen ilmenevä välitön myrkyllisyys:
English: the average mortality for the total number of controls must not exceed 10 % at the end of the test, - the LD50 of the toxic standard meets the specified range.
Finnish: keskimääräinen kuolleisuus kaikilla kontrolleilla ei ole suurempi kuin 10 % testin lopussa; ja - myrkyllisen standardin LD50 on määritellyissä rajoissa.
English: Collection of bees Young adult worker bees of the same race should be used, i.e. bees of the same age, feeding status, etc. Bees should be obtained from adequately fed, healthy, as far as possible disease-free and queen-right colonies with known history and physiological status. They could be collected in the morning of use or in the evening before test and kept under test conditions to the next day. Bees collected from frames without brood are suitable. Collection in early spring or late autumn should be avoided as the bees have a changed physiology during this time. If tests must be conduced in early spring or late autumn, bees can be emerged in an incubator and reared for one week with "bee bread" (pollen collected from the comb) and sucrose solution. Bees treated with chemical substances, such as antibiotics, anti-varroa products, etc., should not be used for toxicity test for four weeks from the time of the end of the last treatment.
Finnish: Mehiläisten kerääminen Testissä olisi käytettävä samaa rotua olevia täysikasvuisia työmehiläisiä. Niiden pitäisi olla saman ikäisiä, samalla tavalla ruokittuja jne. Testimehiläisten olisi oltava asianmukaisesti ruokittuja ja hyväkuntoisia, niissä ei saisi olla tauteja ja ne olisi otettava emollisista pesistä. Yhdyskuntien fysiologinen tila ja aiempi historia pitäisi tuntea. Mehiläiset olisi kerättävä sen päivän aamuna, jolloin ne käytetään, tai edellisenä iltana ja pidettävä testiolosuhteissa seuraavaan päivään. Sikiöttömiltä kakuilta kerätyt mehiläiset ovat sopivia. Keräämistä aikaisin keväällä tai myöhään syksyllä olisi vältettävä, koska mehiläisten fysiologia on erilainen näinä aikoina. Jos testit on tehtävä aikaisin keväällä tai myöhään syksyllä, mehiläisten voi antaa kuoriutua lämpökaapissa ja niitä voi kasvattaa viikon verran "mesileivällä" (kakusta kerätyllä siitepölyllä) ja sakkaroosiliuoksella. Kemiallisilla aineilla kuten antibiooteilla ja varroapunkkilääkkeillä käsiteltyjä mehiläisiä ei saisi käyttää myrkyllisyystesteihin neljään viikkoon viimeisen käsittelyn lopusta.
English: The relative humidity, normally around 50-70 %, should be recorded throughout the test. Handling procedures, including treatment and observations may be conducted under (day) light.
Finnish: Suhteellinen kosteus, jonka pitäisi olla normaalisti 50-70 %, olisi rekisteröitävä koko testin ajan. Käsittelytoimenpiteet ja havainnot voidaan suorittaa (päivän) valossa.
English: Preparation of bees The collected bees are randomly allocated to test cages, which are randomly placed in the experimental room. The bees may be starved for up to 2 h before the initiation of the test. It is recommended that the bees are deprived of food prior to treatment so that all bees are equal in terms of their gut contents at the start of the test. Moribund bees should be rejected and replaced by healthy bees before starting the test.
Finnish: Mehiläisten valmistelu Kerätyt mehiläiset jaetaan satunnaisesti testihäkkeihin, jotka sijoitetaan satunnaisesti koehuoneeseen. Mehiläisiä voidaan pitää ilman ravintoa enintään 2 tuntia ennen testin alkua. Suositellaan, että mehiläisiä pidetään ilman ravintoa ennen käsittelyä, jotta niiden suolen sisältö olisi samanlainen testin alkaessa. Kuolevat mehiläiset on hylättävä, ja niiden tilalle on otettava terveitä mehiläisiä ennen testin aloittamista.
English: Where the test substance is a water miscible compound this may be dispersed directly in 50 % sucrose solution. For technical products and substances of low water solubility, vehicles such as organic solvent, emulsifiers or dispersants of low toxicity to bees may be used (e.g. acetone, dimethylformamide, dimethylsulfoxide). The concentration of the vehicle depends on the solubility of the test substance and it should be the same for all concentrations tested.
Finnish: Jos testiaine on vesiliukoinen, se voidaan liuottaa suoraan 50-prosenttiseen sakkaroosiliuokseen. Tekniset valmisteet ja huonosti vesiliukoiset aineet voidaan liuottaa esimerkiksi sellaisen orgaanisen liuottimen tai emulgointi- tai dispergointiaineen avulla, jonka myrkyllisyys mehiläisille on vähäinen (esim. asetoni, dimetyyliformamidi, dimetyylisulfoksidi). Liuotinapuaineen pitoisuus riippuu testiaineen liukoisuudesta, ja sen pitäisi olla sama kaikille testattaville pitoisuuksille.
English: methods of preparation of stock and test solutions (the solvent and its concentration must be given, when used), - method of preparation of stock solutions and frequency of renewal (the solubilising agent and its concentration must be given, when used),
Finnish: varasto- ja testiliuosten valmistusmenetelmät (jos on käytetty liuotinta, sen nimi ja pitoisuus on mainittava),
English: INTRODUCTION This toxicity test is a laboratory method, designed to assess the acute contact toxicity of plant protection products and other chemicals to adult worker honeybees.
Finnish: JOHDANTO Tämä myrkyllisyystesti on laboratoriomenetelmä, jonka tarkoituksena on arvioida kasvinsuojeluaineiden ja muiden kemikaalien välitöntä myrkyllisyyttä täysikasvuisille työmehiläisille kosketusaltistuksen jälkeen.
English: the average mortality for the total numbers of controls must not exceed 10 % at the end of the test, - the LD50 of the toxic standard meets the specified range.
Finnish: keskimääräinen kuolleisuus kaikilla kontrolleilla ei ole suurempi kuin 10 % testin lopussa ja - myrkyllisen standardin LD50 on määritellyissä rajoissa.
English: Collection of bees Young adult worker bees should be used, i.e. bees of the same age, feeding status, race etc. Bees should be obtained from adequately fed, healthy, as far as possible disease-free and queen-right colonies with known history and physiological status. They could be collected in the morning of use or in the evening before test and kept under test conditions to the next day. Bees collected from frames without brood are suitable. Collection in early spring or late autumn should be avoided, as the bees have a changed physiology during the time. If tests have to be conduced in early spring or late autumn, bees can be emerged in an incubator and reared for one week with "bee bread" (pollen collected from the comb) and sucrose solution. Bees treated with chemical substances, such as antibiotics, anti-varroa products, etc., should not be used for toxicity test for four weeks from the time of the end of the last treatment.
Finnish: Mehiläisten kerääminen Testiin pitäisi käyttää nuoria täysikasvuisia työmehiläisiä, ts. sellaisia, jotka ovat saman ikäisiä, samalla tavoin ruokittuja, saman rotuisia jne. Testimehiläisten olisi oltava asianmukaisesti ruokittuja ja hyväkuntoisia, niissä ei saisi olla tauteja ja ne olisi kerättävä emollisista pesistä, joiden fysiologinen tila ja aiempi historia tunnetaan. Mehiläiset olisi kerättävä sen päivän aamuna, jolloin ne käytetään, tai edellisenä iltana ja pidettävä testiolosuhteissa seuraavaan päivään. Sikiöttömiltä kakuilta kerätyt mehiläiset ovat sopivia. Keräämistä aikaisin keväällä tai myöhään syksyllä olisi vältettävä, koska mehiläisten fysiologia on erilainen näinä aikoina. Jos testit on tehtävä aikaisin keväällä tai myöhään syksyllä, mehiläisten voi antaa kuoriutua lämpökaapissa ja niitä voi kasvattaa viikon verran "mesileivällä" (kakusta kerätyllä siitepölyllä) ja sakkaroosiliuoksella. Kemiallisilla aineilla kuten antibiooteilla ja varroapunkkilääkkeillä käsiteltyjä mehiläisiä ei saisi käyttää myrkyllisyystesteihin neljään viikkoon viimeisen käsittelyn lopusta.
English: Preparation of bees The collected bees may be anaesthetised with carbon dioxide or nitrogen for application of the test substance. The amount of anaesthetic used and time of exposure should be minimised. Moribund bees should be rejected and replaced by healthy bees before starting the test.
Finnish: Mehiläisten valmistelu Mehiläiset voidaan keräyksen jälkeen anestesoida hiilidioksidilla tai typpikaasulla, jotta testiaine voidaan aplikoida. Anestesia-ainetta on käytettävä mahdollisimman pieni määrä ja mahdollisimman lyhyen aikaa. Kuolevat mehiläiset on hylättävä, ja niiden tilalle on otettava terveitä mehiläisiä ennen testin aloittamista.