FrancophonIA/DGT-Translation_Memory huggingface dataset:
English: 1 ml of this solution contains 1 mg DL-α-tocopherol. (UV control see 5.6.2.3;
French: 1 ml de cette solution contient 1 mg de DL-α-tocophérol (pour le contrôle UV: voir 5.6.2.3;
English: Fit the condenser (4.3) to the flask and immerse the flask in a water-bath with magnetic stirrer (4.7). Heat to boiling and allow to reflux for five minutes.
French: Adapter un réfrigérant (4.3) au ballon et immerger celui-ci dans un bain-marie avec agitateur magnétique (4.7).
English: Standard substance: tryptophan (purity/content >= 99 %) dried under vacuum over phosphorous pentoxide
French: Substance-étalon: tryptophane (pureté/teneur >= 99 %) séché sous vide sur diphosphore pentoxyde
Latvian: Standarta substance: triptofāns (tīrība/saturu ≥ 99 %) žāvēts vakuumā virs fosfora pentoksīda
Maltese: triptofan (puritá/kontenut > = 99 %) imnixxef taħt frugħa fuq pentossidu fosfru
English: Internal standard substance: α-methyl-tryptophan (purity/content >= 99 %), dried under vacuum over phosphorous pentoxide
French: Étalon interne proprement dit: α-méthyl-tryptophane (pureté/teneur >= 99 %), séché sous vide sur diphosphore pentoxyde
Bulgarian: Вътрешен еталон в точното му значение : α-метил-триптофан (чистота/съдържание >_ 99%), изсушен във вакуум при наличие на дифосфорен петоокис (дифосфор пентоксид)
English: Mobile phase for HPLC: 3,00 g acetic acid (3.18) + 900 ml water (3.1) + 50,0 ml solution (3.21) of 1,1,1-trichloro-2-methyl-2-propanol (3.19) in methanol (3.8) (1 g/100 ml).
French: Phase mobile pour CLHP: 3,00 g d'acide acétique (3.18) + 900 ml d'eau (3.1) + 50,0 ml de solution (3.21) de trichloro-1,1,1-méthyl-2-propanol-2 (3.19) dans du méthanol (3.8) (1 g/100 ml).
Portuguese: Fase móvel para a HPLC: 3,00 g de ácido acético (3.18) + 900 ml de água (3.1) + 50,0 ml da solução 1 g/100 ml (3.21) de 1,1,1-tricloro-2-metil-2-propanol (3.19) em metanol (3.8).
Dutch: Mobiele fase voor HPLC: 3,00 g azijnzuur (3.18) + 900 ml water (3.1) + 50,0 ml oplossing (3.21) van 1,1,1-trichloor-2-methyl-2-propanol (3.19) in methanol (3.8) (1g/100 ml).
Czech: Mobilní fáze pro HPLC: 3,00 g kyseliny octové (3.18) + 900 ml vody (3.1) + 50,0 ml roztoku (3.21) 1,1,1-trichloro-2-methyl-2-propanolu (3.19) v metanolu (3.8) (1g/100 ml).
Polish: Faza ruchoma do HPLC: 3,00 g kwasu octowego (ppkt 3.18) + 900 ml wody (ppkt 3.1) + 50,0 ml roztworu (ppkt 3.21) 1,1,1-trichloro-2-metylo-2-propanolu (ppkt 3.19) w metanolu (ppkt 3.8) (1 g/100 ml).
English: The sample is ground to pass through a 0,5 mm sieve. Samples high in moisture must be either air-dried at a temperature not exceeding 50.oC or freeze dried prior to grinding.
French: Broyer l'échantillon pour le faire passer dans un tamis de 0,5 mm. Les échantillons à forte teneur en humidité doivent être séchés à l'air à une température de 50 °C au maximum ou par lyophilisation avant broyage.
English: Vitamin E is separated on a C18 reversed phase column (4.5.1) and the concentration is measured using a fluorescence detector (excitation:
German: Bestimmung durch HPLC Die Abtrennung von Vitamin E erfolgt mit Hilfe einer Umkehrphasen-C18-Säule (4.5.1), und die Konzentration wird mit Hilfe eines Fluoreszenzdetektors (Anregung:
Italian: La vitamina E viene separata su una colonna a fase inversa C18 (4.5.1) e la concentrazione viene misurata mediante rivelatore a fluorescenza (eccitazione:
Slovenian: Vitamin E ločimo na koloni C18 z reverzno fazo (4.5.1), koncentracijo izmerimo s fluorescenčnim detektorjem (vzbujanje:
Lithuanian: E vitaminas atskiriamas C18 atvirkštinių fazių kolonėlėje (4.5.1), koncentracija matuojama naudojant fluorescencinį detektorių (sužadinimas:
English: 295 nm, emission:
German: 295 nm, Emission:
Italian: 295 nm, emissione:
Slovenian: 295 nm, emisija:
Lithuanian: 295 nm, emisija:
English: 330 nm) or a UV detector (292 nm) (4.5.2).
German: 330 nm) oder eines UV-Detektors (292 nm) (4.5.2) gemessen.
Italian: 330 nm) (4.5.2) o un rivelatore UV (292 nm) (4.5.2).
Slovenian: 330 nm) ali z UV-detektorjem (292 nm) (4.5.2).
Lithuanian: 330 nm) ar UV detektorių (292 nm) (4.5.2).
Maltese: (4.5.2).330 nm) jew detettur UV (292 nm) (4.5.2).
English: Inject an aliquot portion (e.g. 20 μl) of the methanolic solution obtained in 5.4 and elute with the mobile phase (3.9).
French: Injecter une portion aliquote (par exemple 20 μl) de la solution méthanolique obtenue sous 5.4 et éluer avec la phase mobile (3.9).
German: Hierzu wird ein aliquoter Teil (z. B. 20 μl) der nach 5.4 erhaltenen methanolischen Lösung auf die Trennsäule gegeben und mit der mobilen Phase (3.9) eluiert. Die mittlere Peakhöhe (-fläche) von mehreren Einspritzungen derselben Probenlösung wird ermittelt.
Italian: Iniettare un'aliquota (ad esempio 20 μl) della soluzione di metanolo ottenuta secondo 5.4 ed eluire con la fase mobile (3.9).
Hungarian: Fecskendezzünk be az 5.4. lépés szerint nyert metil-alkoholos oldatból egy aliquot részt (pl. 20 μl), és eluáljuk a mozgó fázissal (3.9.).
Slovenian: Vbrizgamo alikvot (npr. 20 μl) metanolne raztopine, dobljene v skladu s 5.4, in eluiramo z mobilno fazo (3.9).
Romanian: Se injectează o parte alicotă (de exemplu 20 μl) din soluția metanolică obținută la punctul 5.4 și se face o eluție cu faza mobilă (3.9).
Bulgarian: Впръсквате аликвотна част (например 20 µl) от получения метанолов разтвор (виж точка 5.4) и елюирате с мобилната фаза (3.9).
Latvian: Alikvotu porciju (, piem., 20 μl) iesmidzina metanola šķīdumā, kas iegūts 5.4 un eluē ar kustīgo fāzi (3.9).
Lithuanian: Alikvotinę metanolinio tirpalo, gauto 5.4, dalį (pvz., 20 μl) įšvirkškite ir plaukite judančiąja faze (3.9).
Maltese: Injetta porzjoni alikwota (p.e. 20 l) ta' soluzzjoni metanolika miksuba f' 5.4 u elute bil-fażi mobbli (3.9
English: For samples with low vitamin A concentration it may be useful to combine the light petroleum-extracts of two saponification-charges (amount weighed:
Italian: Per campioni con debole concentrazione di vitamina A può essere utile combinare gli estratti di etere di petrolio delle due cariche di saponificazione (quantità pesata:
Slovenian: Za vzorce z nizko koncentracijo vitamina A je morda koristno združiti ekstrakte petroletra iz dveh umilitvenih šarž (natehtana množina:
Romanian: Pentru eșantioanele cu un conținut redus de vitamina A, poate fi utilă combinarea extractelor în eter de petrol provenite din două saponificări (cantitate cântărită:
Maltese: Għal kampjuni b'konċentrazzjoni baxxa ta' vitamina A huwa ta' użu li tikkombina l-estratti ta' pitrolju ħafif ta' żewġ saponifikazzjonijiet-imposti (ammont peżat:
English: 25 g) to one sample solution for HPLC-determination.
Italian: 25 g) con una soluzione campione, per la determinazione HPLC.
Slovenian: 25 g) v eno raztopino vzorca za določanje s HPLC.
Romanian: 25 g) într-o soluție eșantion pentru dozarea prin CLHP
Maltese: 25 g) to one sample solution for HPLC-determination.25 g) għal soluzzjoni waħda ta' kampjun għal determinazzjoni -HPLC.
English: mixture of methanol (3.3) and water, e.g. 980 + 20 (v + v).
German: Mischung von Methanol (3.3) und Wasser, z. B. 980 + 20 (v + v).
Italian: miscela di metanolo (3.3) e acqua, ad esempio 980 + 20 (v + v).
Hungarian: metil-alkohol (3.3.) és víz keveréke, pl. 980 + 20 (v + v).
Slovenian: mešanica metanola (3.3) in vode, npr. 980 + 20 (v + v).
Romanian: amestec de metanol (3.3) și apă, de exemplu 980 + 20 (v + v).
Bulgarian: смес от метанол (метилов спирт) (3.3) и вода, например : 980 + 20 (v + v).
Lithuanian: metanolio (3.3) ir vandens mišinys, pvz., 980 + 20 (V + V).
Maltese: taħlit ta' metanol (3.3) u ilma, per eżempju 980 + 20 (v + v).
English: Graduated flasks with ground-glass stoppers, narrow-necked, 10, 25, 100 and 500 ml 4.2.3.
Maltese: 4.2.2Kunjetti gradwati bit-tappijiet tal-ground-glass, bl-għonq dejjaq, 10, 25, 100 u 500 ml
English: Pleated filter paper for phase separation, diameter 185 mm (e.g. Schleicher & Schuell 597 HY 1/2) 4.5.
Maltese: Karta filtru ippjegata għal separazzjoni tal-fażi, dijametru 185 mm (p.e. Schleicher... Schuell 597 HY 1/2)
English: Vitamin E is separated on a C18 reversed phase column (4.5.1) and the concentration is measured using a fluorescence detector (excitation: 295 nm, emission:
Maltese: Vitamina E hija separata fuq kolonna ta' fażi bil-kontra Ċ18 (4.5.1) u l-konċentrazzjoni hija miżurata bl-użu ta' detettur florexxenti (eċċitazzjoni:
English: Inject an aliquot portion (e.g. 20 μl) of the methanolic solution obtained in 5.4 and elute with the mobile phase (3.8).
French: Injecter une portion aliquote (par exemple 20 μl) de la solution méthanolique obtenue sous 5.4 et éluer avec la phase mobile (3.8).
German: Hierzu wird ein aliquoter Teil (z. B. 20 μl) der nach 5.4 erhaltenen methanolischen Lösung auf die Trennsäule gegeben und mit der mobilen Phase (3.8) eluiert. Die mittleren Peakhöhen (-flächen) von mehreren Einspritzungen derselben Probenlösung werden ermittelt.
Italian: Iniettare un'aliquota (ad esempio 20 μl) della soluzione di metanolo ottenuta secondo 5.4 ed eluire con la fase mobile (3.8).
Hungarian: Fecskendezzünk be az 5.4. pont szerinti metil-alkoholos oldatból egy aliquot részt (pl. 20 μl), és mossuk a mozgó fázissal (3.8.).
Slovenian: Vbrizgamo alikvot (npr. 20 μl) metanolne raztopine, dobljene v skladu s 5.4, in eluiramo z mobilno fazo (3.8).
Romanian: Se injectează o parte alicotă (de exemplu 20 μl) din soluția metanolică obținută la punctul 5.4 și se face o eluție cu faza mobilă (3.8).
Bulgarian: Впръсквате аликвотна част (например 20 µl) от получения метанолов разтвор (виж точка 5.4) и елюирате с мобилната фаза (3.8).
Latvian: Alikvotu porciju (piemēram, 20 μl) iesmidzina metanola šķīdumā, kas iegūts 5.4 un eluē ar kustīgo fāzi (3.8).
Lithuanian: Alikvotinę metanolinio tirpalo, gauto 5.4, dalį (pvz., 20 μl) įšvirkškite ir plaukite judančiąja faze (3.8).
Maltese: Injetta porzjon alikwota (p.e. 20 l) ta' soluzzjoni metanolika miksuba f' 5.4 u elute bil-fażi mobbli (3,8).
English: PART C DETERMINATION OF TRYPTOPHAN
Maltese: PARTI Ċ DETERMINAZZJONI TA' TRIPTOFAN
English: Ethanolamine >
German: Ethanolamin >
Italian: Etanolammina >
Maltese: Etanolamina > 98 %
English: Shake or mix for 60 min.
Maltese: Ħawwad għal 60 min. b;
English: using a mechanical shaker or a magnetic stirrer (4.7).
Maltese: użu ta' ħawwad mekkaniju jew ħawwad manjetiku (4.7)..
English: Proceed to the chromatography step according to paragraph 5.
Maltese: Ipproċedi għal fażi tal-kromatografija skond il-paragrafu 5.
English: All-trans-vitamin A acetate, extra pure, of certified activity, e.g. 2,80 x 106 IU/g
French: Vitamine A acétate all-trans, extra-pure, d'activité garantie, par exemple: 2,80 x 106 UI/g
German: All-trans-Vitamin-A-Acetat, reinst, mit zertifizierter Aktivität, z. B. 2,80 x 106 IE/g
Portuguese: Vitamina A totalmente trans na forma de acetato, extra pura, de actividade certificada, por exemplo 2,80 x 106 UI/g
Dutch: Zuiver trans-vitamine-A-acetaat, extra gezuiverd, van gecertificeerde werking, bijv. 2,80 x 106 IU/g
Finnish: All-trans-A-vitamiiniasetaatti, analyysilaatua, jonka aktiivisuus on varmennettu, esim. 2,80 x 106 ky/g.
Czech: All-trans-vitamín A acetát, extra čistý, ověřené aktivity, např. 2,80 x 106 IU/g
Slovak: All-trans-vitamín A acetát, najvyššej čistoty s potvrdenou aktivitou, napr. 2,80 x 106 IU/g
Polish: Octan all-trans-witaminy A, o specjalnej czystości i certyfikowanej aktywności, np. 2,80 x 106 IU/g
Hungarian: All-trans-A-vitamin-acetát, fokozott tisztaságú, igazolt aktivitású, pl. 2,80 × 106 NE/g
Slovenian: Acetat celotnega trans-vitamina A, posebne čistosti, certificirane aktivnosti, npr. 2,80 x 10.6 IU/g
Romanian: Acetat de vitamina A all-trans, extra pur, cu activitate garantată, de exemplu 2,80 x 106 UI/g
Bulgarian: Витамин А асетат all-trans, с изключителна чистота, гарантирана активност, например 2,80 ха 10 на 6 UI/г
Estonian: Täielikult trans-konfiguratsioonis A-vitamiini atsetaat, eriti puhas, kontrollitud aktiivsusega, näit 2,80 ×106 RÜ/g
Latvian: All-trans- A vitamīna acetāts, īpaši tīrs ar sertificētu aktivitāti, piem., 2,80 x 106 s. v./g
Lithuanian: Visos-trans-A vitamino acetatas, ypač grynas, sertifikuoto aktyvumo, pvz., 2,80 × 106 IU/g
Maltese: Aċetat-trans-vitamina A, extra pur, ta' attivitá awtentikata, per eżempju 2,80 x 106 IU/g
English: All-trans-vitamin A palmitate, extra pure, of certified activity, e.g. 1,80 x 106 IU/g
French: Vitamine A palmitate all-trans, extra-pure, d'activité garantie, par exemple: 1,80 x 106 UI/g
German: All-trans-Vitamin-A-Palmitat, reinst, mit zertifizierter Aktivität, z. B. 1,80 x 106 IE/g
Italian: Palmitato di vitamina A tutto trans, purissimo, di attività certificata, ad esempio 1,80 x 106 UI/g
Portuguese: Vitamina A totalmente trans na forma de palmitato, extra pura, de actividade certificada, por exemplo 1,80 x 106 UI/g
Dutch: Zuiver trans-vitamine-A-palmitaat, extra gezuiverd, van gecertificeerde werking, bijv. 1,80 x 106 IU/g
Finnish: All-trans-A-vitamiinipalmitaatti, analyysilaatua, jonka aktiivisuus on varmennettu, esim. 1,80 x 106 ky/g.
Czech: All-trans-vitamín A palmitát, extra čistý, ověřené aktivity, např. 1,80 x 106 IU/g.
Slovak: All-trans-vitamín A palmitát, najvyššej čistoty s potvrdenou aktivitou, napr. 1,80 x 106 IU/g
Polish: Bardzo czysty palmitynian all-trans-witaminy A o atestowanej aktywności, np. 1,80 x 106 IU/g
Hungarian: All-trans-A-vitamin-palmitát, fokozott tisztaságú, igazolt aktivitású, pl. 1,80 × 106 NE/g
Slovenian: Palmitat celotnega trans-vitamina A, posebne čistosti, certificirane aktivnosti, npr. 1,80 x 10.6 IU/g
Romanian: Palmitat de vitamina A all-trans, extra pur, cu activitate garantată, de exemplu 1,80 x 106 UI/g.
Bulgarian: Витамин А all-trans, с изключителна чистота, гарантирана активност, например 1,80 х 10 на 6 UI/г.
Estonian: Täielikult trans-konfiguratsioonis A-vitamiini palmitaat, eriti puhas, kontrollitud aktiivsusega, näit 1,80 × 106 RÜ/g
Latvian: All-trans- A vitamīna palmitāts, īpaši tīrs ar sertificētu aktivitāti, piemēram, 1,80 x 106 s. v./g
Lithuanian: Visos-trans-A vitamino palmitatas, ypatingai grynas, sertifikuoto aktyvumo, pvz., 1,80 × 106 IU/g
Maltese: Palmitat-trans-vitamina A, extra pur, ta' attivitá awtentikata, per eżempju 1,80 x 106 UI/g
English: Pleated filter paper for phase separation, diameter 185 mm (e.g. Schleicher & Schuell 597 HY 1/2)
French: Papier filtre plissé pour la séparation des phases, d'un diamètre de 185 mm (par exemple Schleicher & Schuell 597 HY 1/2)
German: Phasentrennungsfaltenfilter, Durchmesser 185 mm (z. B. Schleicher & Schuell 597 Hy 1/2)
Italian: Carta filtro pieghettata per separazione di fase; diametro 185 mm (ad esempio Schleicher & Schuell 597 HY 1/2)
Portuguese: Papel de filtro de pregas, com 185 mm de diâmetro, para a separação de fases (por exemplo, Schleicher & Schuell 597 HY 1/2)
Dutch: Geplooid filtreerpapier voor fasescheiding, diameter 185 mm (bijv. Schleicher & Schuell 597 HY 1/2)
Finnish: Laskostettua suodatinpaperia suodatukseen, halkaisija 185 mm (esim. Schleicher & Schüll 597 HY 1/2).
Czech: Skládaný filtrační papír pro oddělení fáze, průměr 185 mm (např. Schleicher... Schuell 597 HY 1/2)
Slovak: Skladaný filtračný papier na oddelenie fáz, priemer 185 mm (napr. Schleicher... Schuell 597 HY 1/2)
Polish: Karbowany sączek bibułkowy do rozdzielania faz, średnica 185 mm (np. Schleicher... Schuell 597 HY1/2)
Hungarian: Redős szűrőpapír a fázisok szétválasztásához, 185 mm átmérőjű (pl. Schleicher & Schuell 597 HY 1/2)
Slovenian: Naguban filtrirni papir za ločevanje faz, premera 185 mm (npr. Schleicher & Schuell 597 HY 1/2)
Romanian: Hârtie de filtru plisată pentru separarea fazelor, cu diametru de 185 mm (de exemplu Schleicher & Schuell 597 HY 1/2)
Bulgarian: Гофрирана филтрова хартия за сепарация на фазите, диаметър 185 мм (например : Schleicher & Schuell 597 HY 1/2)
Estonian: Kurdfilterpaber faaside eraldamiseks, diameeter 185 mm (nt Schleicher... Schuell 597 HY)
Latvian: Kroku filtrs fāžu atdalīšanai, diametrs 185 mm (piemēram, Schleicher & Schuell 597 HY ½)
Lithuanian: Lankstytasis filtro popierius fazėms atskirti, skersmuo 185 mm (pvz., Schleicher & Schuell 597 HY 1/2)
Maltese: Karta filtru ippjegata għal separazzjoni tal-fażi, dijametru 185 mm (p.e. Schleicher... Schuell 597 HY 1/2)
English: The vitamin A content w in IU/kg of the sample is given by the following formula: in which:
Latvian: A vitamīna saturu w paraugā s. v./kg aprēķina, izmantojot šādu formulu: kur:
English: Vitamin E is sensitive to (UV) light and to oxidation. All operations should be carried out in the absence of light (using amber glassware, or glassware protected with aluminium foil) and oxygen (flush with nitrogen).
Latvian: Vitamīns E ir jūtīgs pret (UV) gaismu un skābekļa iedarbību Visas darbības būtu jāveic, izvairoties no gaismas klātbūtnes (izmantojot brūna stikla traukus vai stikla traukus, kas ir aizsargāti ar alumīnija foliju), kā arī no skābekļa klātbūtnes (noskalo ar slāpekli).
English: UV standardisation of the DL-α-tocopherol stock solution (3.11.1) Dilute 2,0 ml of the DL-α-tocopherol stock solution (3.11.1) to 25,0 ml with ethanol and measure the UV spectrum of this solution against ethanol (3.1) in the spectrophotometer (4.6) between 250 nm and 320 nm.
Latvian: DL-α-tokoferola izejas šķīduma (3.11.1) UV standartizācija 2,0 ml DL-α-tokoferola izejas šķīduma (3.11.1) atšķaida ar etanolu līdz 25,0 ml un šā šķīduma UV spektru spektrofotometrā (4.6) salīdzina ar etanola (3.1) spektru starp 250 nm un 320 nm.
English: The vitamin E content w in mg/kg of the sample is given by the following formula: in which:
Latvian: E vitamīna saturu paraugā aprēķina, izmantojot šādu formulu: kurā:
English: Adjust the pH to 3,0 using sodium hydroxide, c = 1,0 mol/l (3.10). Add sufficient methanol (3.8) to give a concentration of between 10 and 30 % of methanol in the final volume.
Latvian: Izmantojot nātrija hidroksīdu c = 1,0 mol/l (3.10), koriģē pH līmeni līdz 3,0 Pievieno pietiekami daudz metanola (3.8), lai metanola koncentrācija galīgajā tilpumā būtu starp 10 un 30 %.
English: Phenolphthalein solution, β = 2 g/100 ml in ethanol (3.1) 3.8.
Bulgarian: Разтвор на фенолфталеин, β = 2 г/100 мл в етанол (етилов спирт) (3.1)
English: Pipette 5,0 ml of this solution into a 25 ml graduated flask and make up to the mark with 2-propanol (3.8). The nominal concentration of this solution is 6,72 IU vitamin A per ml.
Bulgarian: Капвате с пипета 5,0 мл от този разтвор в градуирана колба от 25 мл и допълвате до съответното деление с пропанол-2 (3.8).
English: DL-α-tocopherol acetate, extra pure, of certified activity 3.10.1. Stock solution of DL-α-tocopherol acetate:
Bulgarian: DL-α-токоферолов ацетат, извънредно чист, с гарантирана активност
English: 1 ml of this solution contains 1 mg DL-α-tocopherol acetate. (UV control see 5.6.1.3; stabilisation see 7.4 observations).
Bulgarian: 1 мл от този разтвор съдържа 1 мг DL-α-токоферолов ацетат (за УВ-контрол : виж точка 5.6.1.3; за стабилизация : вж. забележката в т. 7.4)
English: 1 ml of this solution contains 1 mg DL-α-tocopherol. (UV control see 5.6.2.3; stabilisation see 7.4 observations).
Bulgarian: 1 мл от този разтвор съдържа 1 мг DL-α-токоферол (за УВ-контрол : виж точка 5.6.2.3; за стабилизация : вж. забележката в точка 7.4)
English: m= mass of the test portion in g 7.
Bulgarian: m = масата на опитния материал в г
English: Take 82 ml HCl (3.7) and make up to 1 l with water. 3.13.
Bulgarian: взимате 82 мл НСl (3.7) и доливате с вода до 1 литър
English: HPLC determination The following conditions for isocratic elution are offered for guidance; other conditions may be used, provided they yield equivalent results (see also observations 9.1 and 9.2):
Bulgarian: HPLC дозиране За ориентиране се предлагат следните условия за елюиране; и други условия могат да се прилагат, стига за дадат еквивалентни резултати (виж също така забележките в точки 9.1 и 9.2.
English: Liquid chromatographic column, 250 mm x 4 mm, C18, 5 or 10 μm packing, or equivalent (performance criterion:
German: Flüssigkeitschromatographiesäule, 250 mm x 4 mm, C18, 5 oder 10 μm Korngröße oder gleichwertiges Material (Leistungskriterium:
Italian: Colonna analitica da 250 mm x 4 mm, C18, riempimento 5 o 10 μm, o equivalente (criterio della prestazione:
Slovenian: Kolona za tekočinsko kromatografijo, 250 mm x 4 mm C18, 5 ali 10 μm polnitev, ali enakovredna (kriterij za zmogljivost:
Romanian: Coloană pentru cromatografie lichidă de 250 mm x 4 mm, C18, particule de 5 sau 10 μm, sau echivalent (criteriul de performanță:
English: only a single peak for all retinol isomers under the HPLC-conditions)
German: nur ein Peak für alle Retinol-Isomeren unter diesen HPLC-Bedingungen)
Italian: soltanto un unico picco per tutti gli isomeri di retinolo in condizioni HPLC)
Slovenian: samo en vrh za vse izomere retinola pod pogoji kromatografije HPLC)
Romanian: un singur vârf pentru toți izomerii de retinol în condițiile CLHP)
English: Vitamin A is separated on a C18 reversed phase column (4.5.1) and the concentration is measured by means of a UV detector (325 nm) or a fluorescence detector (excitation:
German: Die Abtrennung von Vitamin A erfolgt mit Hilfe einer Umkehrphasen-C18-Säule (4.5.1), und die Konzentration wird mit Hilfe eines UV-Detektors (325 nm) oder eines Fluoreszenzdetektors (Anregung:
Italian: La vitamina A viene separata su una colonna a fase inversa C18 (4.5.1) e la concentrazione viene misurata mediante rivelatore UV (325 nm) o a fluorescenza (eccitazione:
Slovenian: Vitamin A ločimo na koloni C18 z reverzno fazo (4.5.1) in koncentracijo izmerimo z UV- detektorjem (325 nm) ali fluorescenčnim detektorjem (vzbujanje:
English: 325 nm, emission:
German: 325 nm/Emission:
Italian: 325 nm, emissione:
Slovenian: 325 nm, emisija:
English: >PIC FILE= "L_2000174EN.003701.EPS"> in which:
German: >PIC FILE= "L_2000174DE.003701.EPS">
English: Procedure Note:
German: Durchführung Anmerkung:
English: Remove the remaining solvent with a stream of nitrogen (3.9) and dissolve the residue immediately in a known volume (10-100 ml) of methanol (3.3) (the concentration of DL-α-tocopherol should be in the range 5 μg/ml to 30 μg/ml). 5.5. Determination by HPLC
German: Das restliche Lösungsmittel wird im Stickstoffstrom (3.9) abgeblasen, und der Rückstand wird sofort in einer definierten Menge (10-100 ml) Methanol (3.3) aufgenommen (die Konzentration von DL-α-Tocopherol sollte 5 μg/ml bis 30 μg/ml betragen).
English: β= vitamin E concentration of the sample solution (5.4) in μg/ml V1= volume of sample solution (5.4) in ml
German: β= Vitamin-E-Konzentration der Probenlösung (5.4) in μg/ml V1= Volumen der Probenlösung (5.4) in ml
English: Double distilled water or water of equivalent quality must be used (conductivity <
German: Es ist bidestilliertes Wasser oder Wasser gleichwertiger Qualität zu verwenden (Leitfähigkeit <
Italian: Acqua bidistillata o di qualità equivalente (conduttività <
English: 10 ìS/cm)
German: 10 ìS/cm).
Italian: 10 ìS/cm)
English: Take 2,00 ml concentrated solution of tryptophan (3.15), and 2,00 ml of concentrated internal standard (α-methyl-tryptophan) solution (3.16). Dilute with water (3.1) and methanol (3.8) to approximately the same volume and to approximately the same concentration of methanol (10-30 %) as the finished hydrolysate.
German: 2,00 ml konzentrierte Tryptophan-Lösung (3.15) und 2,00 ml konzentrierte interne Standardlösung (α-Methyl-Tryptophan) (3.16) mit Wasser (3.1) und Methanol (3.8) auf etwa dasselbe Volumen und etwa dieselbe Methanolkonzentration (10-30 %) wie das fertige Hydrolysat verdünnen.
English: This solution must be prepared freshly before use. Protect from direct sunlight during preparation.
German: Diese Lösung muß vor Gebrauch frisch hergestellt werden. Bei der Zubereitung vor direktem Sonnenlicht schützen.
English: Filter a few ml of the solution through a 0,45 μm membrane filter (4.5) before injection on the HPLC column. Proceed to the chromatography step according to paragraph 5.4. Protect standard solution and hydrolysates against direct sunlight.
German: Einige ml der Lösung werden durch ein 0,45-μm-Membranfilter (4.5) filtriert, bevor ein Aliquot auf die HPLC-Säule gegeben wird. Die Chromatographie gemäß Nummer 5.4 durchführen. Standardlösung und Hydrolysate sind vor direktem Sonnenlicht zu schützen.