FrancophonIA/DGT-Translation_Memory huggingface dataset:
English: Residues of acetone might lead to losses of aflatoxin B1 on the C18 cartridge) Add 1 ml of methanol (3.2), swirl the flask to dissolve aflatoxin B1 on the sides of the flask, add 4 ml water, and mix.
Slovak: Pridajte 1 ml metanolu (3.2), rýchlym krúživým pohybom banky obsah premiešavajte, aby sa aflatoxín B1 rozpustil na stenách banky, pridajte 4 ml vody, a premiešajte.
Polish: Dodać 1 ml metanolu (3.2), wirować kolbę w celu rozpuszczenia aflatoksyny B1 na ścianach kolby, dodać 4 ml wody i wymieszać.
Maltese: Residwi ta' l-aċetun jistgħu iwasslu għal-telfien ta' l-aflatossin B1 fuq il-kartuċċa C18). Żid 1 ml ta' metanol (3.2), obrom il-garafina sabiex iddewweb l-aflatossin B1 fuq il-ġemb tal-garafina, żid 4 ml ilma, u ħawwad.
English: High performance liquid chromatography (See Figure 2 for setting-up of the equipment).
Slovak: (Montáž zariadenia pozri na obrázku 2).
Polish: Wysokosprawna chromatografia cieczowa (patrz: wykres 2 w celu przygotowania wyposażenia).
English: Inject the aflatoxin B1 calibration solutions (3.13.1 to 3.13.4). Every third injection use the reference standard (3.13.3), for correction of drift in response (NB Peak responses for this reference standard must not differ by more than 10 % in 90 minutes).
Polish: Wstrzyknąć roztwór wzorcowy aflatoksyny B1 (3.13.1 do 3.13.4). co trzecie wstrzyknięcie wykorzystuje wzorzec odniesienia jednostki miary (3.13.3), w celu korekty osadu w odpowiedzi (NB Odpowiedzi pików na ten wzorzec odniesienia jednostki miary nie może różnić się o więcej niż 10 % w ciągu 90 minut).
English: The calibration graph should be linear and pass through the origin, within twice standard error of Y-estimate. Values found must not differ by more than 3 % from the nominal values.
Polish: Wykres kalibracji powinien być liniowy i przechodzić przez oryginał, w ramach dwóch standardowych błędów szacunków Y. Znalezione wartości nie mogą różnić się o więcej niż 3 % od wartości nominalnych.
English: Inject the purified sample extracts
Polish: Wstrzyknąć oczyszczone ekstrakty próbek
English: A small funnel (diameter:
Slovak: Malý lievik (priemer:
Polish: Mały lejek rozdzielczy (średnica:
Maltese: Lembut żgħir (diametru:
English: 4 cm) can be used with a piece of cottonwool in the constriction, covered with a approximately 1 g sodium sulphate.
Slovak: 4 cm) môže sa použiť s kúskom bavlny v priehradke, pokrytom približne 1 g síranu sodného.
Polish: 4 cm) może być wykorzystany z kawałkiem bawełny w swojej konstrukcji, pokrytym około 1 g siarczanu sodowego.
Maltese: 4 cm) jista jkun wżat ma biċċa tajjar fil-kostrizzjoni, mgħotti b'madwar 1 g sulfat tas-sodju.
English: Preparation of derivative and thin layer chromatography: See Annex to Council Directive 76/372/EEC method A, point 5.6.2.
Polish: Przygotowanie instrument pochodny oraz chromatografii cienkowarstwowej. Patrz Załącznik dyrektywy Rady 76/372/EWG metoda A, punkt 5.6.2.
English: aflatoxin B1 content in mg/kg = m × Ve x t Vm × M × Vf Vc where:
Polish: Zawartość aflatoksyny B1 w μg/kg = m × V V × M × VfVc gdzie:
Slovenian: vsebnost aflatoksina B1 v m × V V × M × VfVc pri čemer je:
Estonian: aflatoksiin B1 sisaldus (μg/kg) = m × V V × M × VfVc kus:
Latvian: Aflatoksîna B1 saturs, izsakot μg/kg = m × V V × M × VfVc kur
English: M = mass of sample in g
Slovak: M = hmotnosť vzorky v g
Polish: M = masa próbki w g
Estonian: M = proovi mass, g;
English: Vf = volume of filtrate transferred to Florisil cartridge (5.4.1.2) in ml
Slovak: Vf = objem filtrátu prenesený na florisilovú patrónu (5.4.1.2) v ml
Polish: Vf = ilość filtratu przelanego do filtra Florisil (5.4.1.2) w ml
Estonian: Vf = Florisil-padrunisse kantud filtraadi maht (5.4.1.2), ml;
English: see under 10.1 9.
Slovak: pozri v bode 10.1.9.
Polish: Patrz: punkt 10.1
Estonian: vt punkt 10.1.
Maltese: Ara taħt 10.1 9.
English: see under 10.1 10.
Polish: Patrz: punkt 10.1
Maltese: Ara taħt 10.1 10.
English: A collaborative study (1), carried out at the international level on mixed feeding stuffs gave the results for repeatability and reproducibility indicated in Table 1. The term repeatability (r) used here is defined as the largest ratio which is not significant at the 95 % probability level for comparison of two readings of the same sample in the same laboratory under similar conditions.
Polish: Wspólne studium [1], przeprowadzone na poziomie międzynarodowym na mieszanych paszach dostarczyło wyników w odniesieniu do powtarzalności i odtwarzalności wskazanej w tabeli 1. Pojęcie powtarzalność r) użyte tutaj jest zdefiniowane jako największy stosunek, który nie jest istotny przy 95 % poziomu prawdopodobieństwa w odniesieniu do porównania dwóch odczytów tej samej próbki w tym samym laboratorium na podobnych warunkach.
English: Repeatability (r) and reproducibility (R) expressed as ratios and corresponding coefficients of variation (15 laboratories) Level r R CVr (*) CVR (mg/kg) (%) (%) 8 & 14 1,4 1,7 11 18 (*) CV = coefficient of variation
Slovak: Opakovateľnosť r) a reprodukovateľnosť (R) vyjadrené ako pomery a zodpovedajúce variačné koeficienty (15 laboratórií) Úroveň r R CVr CVR (μg/kg) (%) (%)
Polish: Powtarzalność (r) i odtwarzalność (R) wyrażona jako stopy i odpowiednie współczynniki zmian (15 laboratoriów) Poziom r R CV, f CVR (µg/kg) (%) (%)
English: Accuracy The correct applications of the method shall be verified by making replicate measurements on certified reference materials.
Polish: Dokładność Poprawne zastosowania metody powinny być zweryfikowane poprzez powielone pomiary certyfikowanego materiału odniesienia.
English: Principle The sample is extracted with chloroform.
Slovak: Podstata metódy Vzorka sa extrahuje chloroformom.
Latvian: Princips Paraugu ekstrahē ar hloroformu.
English: Note: mycotoxins are extremely toxic sustances.
Latvian: Piezīme: mikotoksīni ir ārkārtīgi toksiskas vielas.
English: Saturated iodine solution: add 2 g of iodine to 400 ml of water.
Latvian: Piesātināts joda šķīdums: 400 ml ūdens pievienojiet 2 g joda.
English: Check the concentration of the solution as follows: determine the absorption spectrum of the solution between 330 and 370 nm by means of the spectrophotometer (4.23).
Latvian: Šā šķīduma koncentrāciju pārbauda šādi: nosaka šķīduma absorbcijas spektru no 330 līdz 370 nm, izmantojot spektrofotometru (4.23.).
English: Concentration (mg/ml) = 312 × A × 1000 22 300 = 13,991 × A
Latvian: Koncentrâcija (μg/ml) = = 13,991 × A
English: Aflatoxine B1 stock standard solution in chloroform. Transfer quantitatively 2,5 ml of the aflatoxin B1 standard solution (3.12) to a 50 ml volumetric flask and adjust to the mark with chloroform (3.1).
Latvian: Aflatoksīna B1 rezerves standartšķīdums hloroformā. Kvantitatīvi pārnes 2,5 ml aflatoksīna B1 standartšķīduma (3.12.) uz 50 ml mērkolbu un koriģē līmeni līdz zīmei ar hloroformu (3.1.).
Maltese: Aflatossin B1 ħażna ta' taħlita normali fil-kloroform Trasferixxi b'mod kwanitattiv 2,5 ml tat-taħlita normali ta' l-aflatossin B1 (3.12) lejn garafina volumetrika 50 ml u aġġusta l-marka bil-kloroform (3.1).
English: Spiral reaction coil; Teflon or stainless steel.
Estonian: Spiraalne reaktsioonispiraal, teflonist või roostevabast terasest.
Latvian: Spirālveida reakcijas spirāle no teflona vai nerūsējoša tērauda.
English: ISO 3534-1977. (i) UV absorbing foil on the windows in combination with subdued light (no direct sunlight);
Latvian: UV absorbējošu foliju uz logiem kombinācijā ar samazinātu apgaismojumu (bez tiešas dienasgaismas);
English: Set the fluorescence detector (4,11) to exc. = 365 nm and em = 435 nm (filter instrument;
Slovak: Nastavte fluorescenčný detektor (4,11) na excitačnú vlnovú dĺžku = 365 nm a em = 435 nm (filtračný nástroj;
Latvian: Fluorescences detektoram (4.11.) uzstāda indukcijas viļņa garumu 365 nm un emisiju viļņa garumu 435 nm (filtra instrumentam
English: Injection of sample extracts Inject the purified sample extracts (5.4.2.2).
Czech: Nástřik extraktu vzorku Provede se nástřik přečištěného extraktu (5.4.2.2).
Slovenian: Vbrizganje ekstraktov vzorcev Vbrizgamo očiščene ekstrakte vzorca (5.4.2.2).
Estonian: Prooviekstraktide süstimine kromatograafi Süstida kromatograafi puhastatud prooviekstraktid (5.4.2.2).
Latvian: Parauga ekstraktu injekcija Injicē attīrītus parauga ekstraktus (5.4.2.2.).
Maltese: Injezzjoni ta' l-estratti tal-kampjun: Injetta l-estratti tal-kampjun purifikat (5.4.2.2).
English: Calculation of results Calculate the aflatoxin B1 content (mm/kg) present in the sample, using the formula:
Slovenian: Izračun rezultatov Vsebnost aflatoksina B1(mg/kg) v vzorcu izračunamo s formulo:
Latvian: Rezultātu aprēķināšana Aflatoksīna B1 saturu (μm/kg) paraugā aprēķina, izmantojot šādu formulu:
Maltese: Kalkolazzjoni tar-riżultati tal-kontenut aflatossin B1 (mm/kg) preżenti fil-kampjun, bl-użu tal-formula:
English: m = amount of aflatoxin B1 in ng represented by the B1 peak of the sample, calculated as follows: m = P (sample) P (st1) + P (st2) × 2 r (st) P (sample) = peak area of aflatoxin B1 for the sample P (st1) = peak area of aflatoxin B1 resulting from the preceeding injection of reference standard (3.13.3) P (st2) = peak area of aflatoxin B1 resulting from the following injection of reference standard (3.13.3) r (st) = injected amount of aflatoxin B1 in the reference standard (3.13.3) in ng Vm = volume of the injected sample extract in ml Ve x t = final volume of sample extract in ml, allowing for any dilution that was made (5.3) M = mass of sample in g Vf = volume of filtrate transferred to Florisil cartridge (5.4.1.2) in ml Vc = volume of chloroform, used for the extraction of the sample in ml
Latvian: m m = P + P × 2 r st P (paraugs) = parauga B1 maksimuma zona P(st1) = aflatoksīna B1 maksimuma zona, kas rodas iepriekšējās standartvielas (3.13.3.) injekcijas rezultātā P(st2) = aflatoksīna B1 maksimuma zona, kas rodas nākamās standartvielas (3.13.3.) injekcijas rezultātā r(st) = standartvielā (3.13.3.) injicētais aflatoksīna B1 daudzums, izsakot ng Vm = injicētā parauga ekstrakta daudzums mililitros Vext = parauga ekstrakta galīgais tilpums mililitros, no kura var sagatavot jebkuru vajadzīgo atšķaidījumu (5.3.) M = parauga masa gramos Vf = uz florisila kaseti (5.4.1.2.) pārnestā filtrāta tilpums mililitros Vc = parauga ekstrahēšanai izmantotā hloroforma daudzums mililitros
English: If the procedure is followed as in this protocol, the formula reduces to: aflatoxin B1 content in mg/kg = 20 × m.
Latvian: Ja procedūru izpilda atbilstoši šim protokolam, formulu noīsinās līdz: aflatoksīna B1 saturs, izsakot μg/kg = 20 × m.
English: Repeatability: see under 10.1 9.
Latvian: Atkārtojamība: skatīt 10.1. punktu. 9.
English: Reproducibility: see under 10.1
Latvian: Reproducējamība: skatīt 10.1. punktu.
English: Repeatability (r) and reproducibility (R) expressed as ratios and corresponding coefficients of variation
Estonian: Korduvus (r) ja korratavus (R) väljendatuna suhtarvudena ning neile vastavate variatsioonikordajatena.
Latvian: Atkārtojamība (r) un reproducējamība (R), kas izteiktas kā attiecības un attiecīgie variāciju koeficenti
English: (15 laboratories)
Estonian: (15 laborit)
Latvian: (15 laboratorijas)
English: Level
Latvian: Līmenis
English: CVr
Latvian: CVr
English: CVR
Latvian: CVR
English: (mg/kg)
Latvian: (μg/kg)
English: Flow diagram of the LC system with iodine post-column derivatization response mean of adjacent
Latvian: Šķidruma hromatogrāfijas plūsmas shēma ar joda pēckolonnas derivatizāciju
English: Luer chloroform-resistant stopcock (e.g. Bio-rad 7328017, Analytichem A1 6078, J.T. Baker 4514 or equivalent).
Czech: Chloroformu odolný uzavírací kohout Luer (např. Bio-Rad 7328017, Analytichem A1 6078, J.T. Baker 4514) nebo ekvivalent
Slovak: Luerov uzatvárací kohútik odolný proti chloroformu (napr. Bio-rad 7328017, Analytichem A1 6078, J. T. Baker 4514 alebo ekvivalentný).
Polish: Zawór odcinający typu Luer odporny na chloroform (np. Bio-rad 7328017, Analytichem Al 6078, J.T. Baker 4514 lub podobny).
Hungarian: Luer(R) kloroformmal szemben ellenálló csap (pl. Bio-Rad 7328017, Analytichem A1 6078, J.T.Baker 4514 vagy ezekkel egyenértékű).
Estonian: Lueri otsakuga kloroformikindel korkkraan (nt Bio-rad 7328017, Analytichem A1 6078, J.T. Baker 4514 või samaväärne).
Latvian: Pret hloroformu izturīgs Luira noslēdzošais krāns (piemēram, Bio-rad 7328017, Analytichem A1 6078, J.T. Baker 4514 vai līdzvērtīgs).
Lithuanian: Chloroformui atsparus Luer čiaupas (pvz., Bio-rad 7328017, Analytichem A1 6078, J.T. Baker 4514 arba atitikmuo).
Maltese: Stopcock (vit) Luer reżistenti għall-klorofilla (e.g. Bio-rad 7328017, Analytichem A1 6078, J.T. Baker 4514 jew ekwivalenti).
English: Ray MAC SHARRY
Slovak: Ú. v. ES L 246, 29.8.1981, s. 32.
Maltese: Ray Mac Sharry
English: See observation
Slovenian: Glej točko
Maltese: Ara l-osservazzjoni
English: Concentration (mg/ml) = 312 × A × 1000 22 300 = 13,991 × A 3.12.1.
Maltese: Konċentrazzjoni (mg/ml) = = 13,991 × A 3.12.1.
English: Apparatus Caution:
Czech: Přístroje a pomůcky:
Slovak: Aparatúra
Maltese: Twissija dwar l-apparat:
English: Preparation of the sample. Grind the sample so that it passes through the sieve (4.2).
Maltese: Tħejjija tl-kampjun. Isħaq il-kampjun hekk li jkun jista jgħaddi mill-għarbiel (4.2).
English: Purification Add the filtrate collected in 5.3 to the column-cartridge assembly and drain by gravity.
Maltese: Purifikazzjoni: Żid il-filtrat miġbur fi 5.3 ma l-assemplea tal-kartuċċa-kolonna u saffi bil-gravità.
English: Discard the eluates. During these operations, ensure that the column-cartridge assembly does not run dry.
Maltese: Matul dawn l-operazzjonijiet, assigura li l-assemblea tal-kartuċċa-kolonna ma tinxiefx.
English: Disconnect and discard the cartridge. Rinse the glass column with water and retain for the C18 purification step.
Maltese: Neħħi u warrab il-kartuċċa. Laħlaħ il-kolonna tal-ħġieġ bl-ima u żomm għal pass tal-purifikazzjoni C18.
English: HPLC pump settings Set the HPLC pump (4.5) to give a flow of 0,5 or 0,3 ml/min for a 5 mm or a 3 mm HPLC column (4.6) respectively using the mobile phase (3.6).
Maltese: It-tqegħid tal-pompa HPLC: Ikkalibra l-pompa HPLC (4.5) sabiex ikun hemm nixxija 0,5 jew 0,3 ml/min għal 5 mm jew 3 mm kolonna HPLC (4.6) rispettivament bl-użu tal-fażi mobbli (3.6).
English: Post-column pump settings Set the pump (4.7) to give a flow of 0,2 to 0,4 ml/min of the iodine-saturated water solution (3.7).
Maltese: Ikkalibrar tal-pompa ta' wara-il-kolonna: Ikkalibra l-pompa (4.7) sabiex ikun hemm nixxija ta' 0,2 sa 0,4 ml/min ta' taħlita ta' ilma saturat bil-jodju (3.7).
English: Further treatment of the extract (5.4.2.2) Add 5 ml sodium chloride solution (3.15.1) to the final extract obtained at 5.4.2.2.. Extract three times each with 2 ml chloroform (3.1) for one minute, in the separating funnel (4.24.1).
Slovak: Ďalšie ošetrenie extraktu (5.4.2.2) Do konečného extraktu, získaného pri 5.4.2.2., pridajte 5 ml roztoku chloridu sodného (3.15.1). Každý extrahujte v separačnom lieviku trikrát pomocou 2 ml chloroformu (3.1) počas jednej minúty (4.21.1).
Maltese: Aktar trattament ta' l-estratt (5.4.2.2). Żid 5 ml taħlita tal-klorat tas-sodju (3.15.1) sa l-estratt finali akkwistat fi 5.4.2.2. Ħu estratt tlett darbiet kull waħda bi 2 ml kloroform (3.1) għal minuta waħda, fil-lembut tas-separazzjoni (4.24.1).
English: P (st1) + P (st2) × 2 r (st) P (sample) = peak area of aflatoxin B1 for the sample P (st1) = peak area of aflatoxin B1 resulting from the preceeding injection of reference standard (3.13.3) P (st2) = peak area of aflatoxin B1 resulting from the following injection of reference standard (3.13.3) r (st) = injected amount of aflatoxin B1 in the reference standard (3.13.3) in ng Vm = volume of the injected sample extract in ml Ve x t = final volume of sample extract in ml, allowing for any dilution that was made (5.3) M = mass of sample in g Vf = volume of filtrate transferred to Florisil cartridge (5.4.1.2) in ml Vc = volume of chloroform, used for the extraction of the sample in ml If the procedure is followed as in this protocol, the formula reduces to:
Maltese: P (st1) = żona tal-quċċata ta' aflatossin B1 li tiorriżulta mill-injezzjoni preċedenti tat-taħlita normali ta' referenza (3.13.3) P (st2) = żona tal-quċċata ta' aflatossin B1 li tirriżulta mill-injezzjoni segwentu tat-taħlita normali ta' referenza (3.13.3) r (st) = ammont in jettat ta' aflatossin B1 fit-taħlita normali ta' referenza (3.13.3) fib g Vm = volum ta' l-estratt tal-kampjun injettat fi ml V ext = volum final ital-kampjun estratt fi ml, billi tippermetti għal xi trattib li kien sar (5.3) M = massa tal-kampjun fi g Vf = volum tal-filtrat trasferit lejn il-kartuċċa Florisil (5.4.1.2) fi ml Vc = volum tal-kloroform, użat għall-estrazzjoni tal-kampjun fi ml. Jekk il-proċedura tkun segwita bħal f'dan il-protokoll, il-formula tkun imqassra għal:
English: Table 1 Repeatability (r) and reproducibility (R) expressed as ratios and corresponding coefficients of variation (15 laboratories)
Maltese: Tabella 1: Repetabbiltà (r) u reproduċċibbiltà (R) espressi bħala relattivitajiet u ko-effiċjenti korrespondenti tal-varjazzjoni (15 il-laboratorju)
English: Stabilization of chloroform (3.1) The adsorption characteristics of the florisil cartridge may be changed if stabilizers other than ethanol are used.
Maltese: Stabbilizzazzjoni tal-kloroform (3.1): Il-karatteristiċi ta' assorbazzjoni tal-kartiċċa Florisil jistgħi jinbidlu jekk stabilizzaturi oħrajn, apparti milli etanol, ikunu wżati.
English: Flow diagram of the LC system with iodine post-column derivatization response mean of adjacent reference standards time reference standard extract (or calibrant) extract (or calibrant) reference standard extract (or calibrant)
Maltese: Diagramma tan-nixxija tas-sistema LC bil-wara-kolonna tal-jodju, reazzjoni tad-devjazzjoni tal-medja ta' normi ta' referenza tal-biswit, referenza taż-żmien ta' l-estratt normali (jew kalibrant) estratt (jew kalibrant) estratt normali ta' referenza (jew kalibrant).
English: The lower limit of determination is 0,001 mg/kg (1 ppb). 2.
Slovak: Spodná hranica pre stanovenie je 0,001 mg/kg (1 ppb).
English: Concentration (mg/ml) = 312 × A × 1000
Slovak: Koncentrácia (μg/ml) =
English: Store this solution in a cool place (4 °C) in the dark, well sealed and wrapped in aluminium foil. 3.13.
Slovak: Tento roztok skladujte na chladnom tmavom mieste (pri teplote 4 °C), dôkladne utesnený a zabalený v hliníkovej fólii.
English: NB Use alcid-washed glassware for preparation of these solutions (see 4, Apparatus). 3.13.1.
Slovak: Na prípravu týchto roztokov použite laboratórne sklo umyté kyselinou (pozri bod 4 – Aparatúra).
English: Transfer quantitatively 2,5 ml of the calibration solution (3.13.1) to a 10 ml volumetric flask, make up to the mark with the water/acetone mixture (3.5.3) and mix well. 3.14.
Slovak: Kvantitatívne preneste 2,5 ml kalibračného roztoku (3.13.1) do odmernej banky s obsahom 10 ml, doplňte po značku zmesou vody a acetónu (3.5.3) a dobre premiešajte.
English: (For filter instrument:
Czech: (Pro detektory s filtry:
Slovak: (Pre filtrovacie zariadenie:
English: emission wavelength > 400 nm).
Czech: emisní vlnová délka <400 nm).
Slovak: emisná vlnová dĺžka < 400 nm).
English: Egmond, H.P. van, Heisterkamp, S.H. and Paulsch, W.E. (1991). Food Additives and Contaminants 8, 17-29. (2) ISO 3534-1977.
Slovak: Účinne sa to dá urobiť tak, že použijete:
English: Close the stopcock. Remove the syringe.
Slovak: Uzavrite kohútik. odstráňte injekčnú striekačku.
English: Teflon filters are acceptable. 5.5.
Slovak: Vysokoúčinná kvapalinová chromatografia
English: reference standard, extract, extract, reference standard, extract, extract, reference standard etc. 6.
Slovak: referenčný etalón, extrakt, extrakt, referenčný etalón, extrakt, extrakt, referenčný etalón atď.
English: Confirmatory test 6.1.
Slovak: Overovacia skúška
Estonian: Kinnitav katse
English: P (st1) + P (st2) × 2 r (st) P (sample) = peak area of aflatoxin B1 for the sample
Slovak: st P (vzorka) = plocha pod zónou aflatoxínu B1 pre vzorku
English: I. Under Part A 'One-dimensional thin layer chromatographic method' the text at point 1 'Purpose and scope' is replaced by the following text:
Czech: I. V části A "Metoda jednorozměrné chromatografie na tenké vrstvě" se text v bodě 1 "Účel a oblast použití" nahrazuje tímto:
Slovenian: I. V delu A "Enodimenzionalna tankoplastna kromatografska metoda" se besedilo v točki 1 "Namen in področje uporabe" nadomesti z naslednjim besedilom:
Estonian: I. A osas "Ühemõõtmeline planaarkromatograafiline meetod" asendatakse tekst punktis 1 "Eesmärk ja rakendusala" järgmise tekstiga:
English: 'B. DETERMINATION OF AFLATOXIN B1. HIGH PERFORMANCE LIQUID CHROMATOGRAPHIC METHOD
Slovenian: "B. DOLOČANJE AFLATOKSINA B1 Z METODO TEKOČINSKE KROMATOGRAFIJE VISOKE LOČLJIVOSTI
English: (For filter instrument emission wavelength > 400 nm).
Slovenian: (Za filtrirni instrument valovna dolžina emisije > 400 nm).
English: Luer chloroform-resistant stopcock (e.g. Bio-rad 7328017, Analytichem A1 6078, J.T.
Slovenian: Luer petelinček, odporen na kloroform (npr. Bio-rad 7328017, Analytichem Al 6078, J.T.
English: Baker 4514 or equivalent).
Slovenian: Baker 4514 ali enakovreden).
English: Prime the cartridge and remove any air by passing 10 ml methanol (3.2) via the stopcock rapidly through the cartridge with a syringe (4.19).
Slovenian: Nastavimo kartušo in odstranimo zrak, tako da hitro pretočimo 10 ml metanola (3.2) prek petelinčka z uporabo brizge (4.19).
English: Before each series of analyses, respectively inject the reference standard (3.13.3), until stable peak areas are achieved
Slovenian: Pred vsako serijo analiz vbrizgamo referenčni standard (3.13.3), dokler ne dosežemo stabilne površine vrhov.
English: Peak responses for aflatoxin B1 between consecutive injections should not differ by more than 6 %).
Slovenian: Vrhovi aflatoksina B1 med dvema zaporednima vbrizgoma se ne smejo razlikovati za več kot 6 %).
English: A small funnel (diameter 4 cm) can be used with a piece of cottonwool in the constriction, covered with a approximately 1 g sodium sulphate.
Czech: Může být použita např, malá dělicí nálevka (o průměru 4 cm) s kouskem vaty v zúžení, pokrytým síranem sodným.
Slovenian: Uporabimo lahko manjši lij (premer 4 cm) s koščkom surovega bombaža v vratu lija, ki ga pokriva približno 1 g natrijevega sulfata.
English: m = amount of aflatoxin B1 in ng represented by the B1 peak of the sample, calculated as follows m = P (sample) P (st1) + P (st2) × 2 r (st) P (sample) = peak area of aflatoxin B1 for the sample P (st1) = peak area of aflatoxin B1 resulting from the preceeding injection of reference standard (3.13.3) P (st2) = peak area of aflatoxin B1 resulting from the following injection of reference standard (3.13.3) r (st) = injected amount of aflatoxin B1 in the reference standard (3.13.3) in ng Vm = volume of the injected sample extract in ml Ve x t = final volume of sample extract in ml, allowing for any dilution that was made (5.3) M = mass of sample in g Vf = volume of filtrate transferred to Florisil cartridge (5.4.1.2) in ml Vc = volume of chloroform, used for the extraction of the sample in ml If the procedure is followed as in this protocol, the formula reduces to:
Slovenian: m m = P + P × 2 r st P (vzorec) = površina vrha aflatoksina B1 vzorca P(st1) = površina vrha aflatoksina B1, ki izhaja iz predhodnega vbrizganja referenčnega standarda (3.13.3) P(st2) = površina vrha aflatoksina B1, ki izhaja iz naslednjega vbrizganja referenčnega standarda (3.13.3) r(st) = vbrizgana količina aflatoksina B1 v referenčnem standardu (3.13.3) v ng Vm = prostornina vbrizganega ekstrakta vzorca v ml Vext = končna prostornina ekstrakta vzorca v ml, z upoštevanjem opravljene razredčitve (5.3) M = masa vzorca v g Vf = prostornina filtrata, prenesenega v kartušo Florisil (5.4.1.2) v ml Vc = prostornina kloroforma, uporabljenega za ekstrahiranje vzorca v ml Če upoštevamo postopek v tem protokolu, se formula glasi:
English: Pump
Slovenian: Slika 2:
English: DETERMINATION OF AFLATOXIN B1. HIGH PERFORMANCE LIQUID CHROMATOGRAPHIC METHOD
Czech: STANOVENÍ AFLATOXINU B1 METODOU VYSOKOÚČINNÉ KAPALINOVÉ CHROMATOGRAFIE
English: Acetonitril, HPLC grade. 3.5.
Czech: Acetonitril, čistoty HPLC