FrancophonIA/DGT-Translation_Memory huggingface dataset:
English: 6,0 g Tryptone4,0 gYeast extract 3,0 g Meat extract 1,5 g Glucose 1,0 g Agar 10,0 to 20,0 g Water 1 000 ml pH 6,5 to 6,6 (after sterilization).
Polish: 6,0 g Trypton 4,0 g Ekstrakt z drożdży 3,0 g Ekstrakt z mięsa 1,5 g Glukoza 1,0 g Agar 10,0-20,0 g Woda 1 000 ml pH 6,5 do 6,6 (po sterylizacji).
English: 10,0 gYeast extract3,0 g Meat extract 1,5 g Glucose 1,0 g Agar 10,0 to 20,0 g Tween 80 1 ml Water 1 000 ml pH 6,5 (after sterilization).
Polish: 10,0 g Ekstrakt z drożdży 3,0 g Ekstrakt z mięsa 1,5 g Glukoza 1,0 g Agar 10,0-20,0 g Tween 80 1 ml Woda 1 000 ml pH 6,5 (po sterylizacji)
English: Potassium hydrogen phosphate K2HPO4 22,15 g. Potassium dihydrogen phosphate KH2PO4 27,85 g. Water to 1 000 ml.
Polish: Wodorofosforan potasu, K2HPO4 22,15 g Diwodorofosforan potasu, KH2PO4 27,85 g Woda do 1 000 ml.
English: Check that the pH is about 2. Shake for 10 minutes.
French: Vérifier que le pH est de 2 environ.
Polish: Sprawdzić, czy pH wynosi około 2. Wstrząsać przez 10 minut.
Slovenian: Preveri se, da je pH vrednost približno 2, in stresa 10 minut.
English: The fact that the result has been calculated from three levels should be noted on the final report. When the lines are considered as being parallel, calculate the logarithm (log A) of the relative activity (A) by means of one of the following formulae, depending upon whether three or four levels have been used for the assessment of parallelism.
Polish: W sprawozdaniu końcowym należy odnotować fakt, że wynik uzyskano przy użyciu trzech poziomów. Jeśli proste zostały uznane za równoległe, należy obliczyć logarytm względnej aktywności (log A) za pomocą jednego z poniższych wzorów, w zależności, czy do oceny równoległości prostych użyto trzech, czy czterech poziomów.
English: // (c) log A = // (u1 + u2 + u4 + u8 - s1 - s2 - s4 - s8) × 0,602 u4 + u8 + s4 + s8 - u1 - u2 - s1 - s2 For three levels
Polish: log A = Dla trzech poziomów
English: When the lines are considered as not being parallel, repeat the determination. If parallelism is still not achieved, a satisfactory determination has not been obtained.
Polish: Jeśli uznano, że proste nie są równoległe, należy powtórzyć oznaczenie. Jeśli proste nadal nie są równoległe, oznaczenie należy uznać za niezadowalające.
English: The difference between the results of two determinations carried out on the same sample by the same analyst should not exceed: - 2 mg/kg, in absolute value, for contents of zinc bacitracin up to 10 mg/kg,
Polish: Różnica między wynikami dwóch równoległych oznaczeń, przeprowadzonych dla tej samej próbki przez tego samego analityka, nie powinna przekraczać:
English: // (a) SL = // 7s1 + 4s2 + s4 - 2s8 10
French: // (a) SL = // 7s1 + 4s2 + s4 - 2s8 10
Czech: 7s1 + 4s2 + s4 - 2s8 (a) SL =
English: // (b) SH = // 7s8 + 4s4 + s2 - 2s1 10
French: // (b) SH = // 7s8 + 4s4 + s2 - 2s1 10
Czech: 7s8 + 4s4 + s2 - 2s1 (b) SH =
English: // (a') SL = // 5s1 + 2s2 - s4 6 // or // 5s2 + 2s4 - s8 6 // (b') SH = // 5s4 + 2s2 - s1 6 // or // 5s8 + 2s4 - s2 6
French: // (a') SL = // 5s1 + 2s2 - s4 6 // ou // 5s2 + 2s4 - s8 6 // (b') SH = // 5s4 + 2s2 - s1 6 // ou // 5s8 + 2s4 - s2 6
Czech: 5s1 + 2s2 - s4 5s1 + 2s2 - s4 (a´) SL = nebo 5s4 + 2s2 - s1 5s8 + 2s4 - s2 (b´) SH = nebo
English: // (c) Log A = // (u1 + u2 + u4 + u8 - s1 - s2 - s4 - s8) × 0,602 u4 + u8 + s4 + s8 - u1 - u2 - s1 - s2
Czech: (u1 + u2 + u4 + u8 - s1 - s2 - s4 - s8) x 0,602 (c) log A = u4 + u8 + s4 + s8 - u1 - u2 - s1 - s2
English: // (d) Log A = // (u1 + u2 + u4 - s1 - s2 - s4) × 0,401 u4 + s4 - u1 - s1 // or // // (d') Log A = // (u2 + u4 + u8 - s2 - s4 - s8) × 0,401 u8 + s8 - u2 - s2
Czech: (u1 + u2 + u4 - s1 - s2 - s4 ) x 0,401 (d) log A = u4 + s4 + - u1 - s1 nebo (u2 + u4 + u8 - s2 - s4 - s8 ) x 0,401 (d´) log A = u8 + s8 + - u2 - s2
English: COMMISSION DIRECTIVE of 20 December 1983 amending Directives 71/393/EEC, 72/199/EEC and 78/633/EEC establishing Community methods of analysis for the official control of feedingstuffs (84/4/EEC) THE COMMISSION OF THE EUROPEAN COMMUNITIES, Having regard to the Treaty establishing the European Economic Community,
Slovenian: DIREKTIVA KOMISIJE z dne 20. decembra 1983 o spremembi direktiv 71/393/EGS, 72/199/EGS in 78/633/EGS o določitvi analitskih metod Skupnosti za uradni nadzor krme (84/4/EGS)KOMISIJA EVROPSKIH SKUPNOSTI JE ob upoštevanju Pogodbe o ustanovitvi Evropske gospodarske skupnosti,
English: Light petroleum, boiling range: 40 to 60 _C.
Slovenian: Petroleter, vrelišče: med 40 in 60 °C.
English: Drying oven, either a vacuum oven set at 75 _ 3 _C or an air-oven set at 100 _ 3 _C.
Slovenian: Sušilna peč; bodisi vakuumska peč, nastavljena na 75 ± 3 °C, ali peč z vročim zrakom, nastavljena na 100 ± 3 °C.
English: Place the double filter paper containing the residue on a watch glass and dry for one and a half hours in the oven at 100 _ 3 _C.
Slovenian: Dvojni filtrirni papir, na katerem je ostanek, se namesti na armirano steklo in poldrugo uro suši v peči pri 100 ± 3 °C.
English: Inoculate tubes containing slopes of culture medium (4.1) with Micrococcus luteus and incubate for 24 hours at 30 _C.
Slovenian: Epruvete s poševnimi nanosi gojišča (4.1) se nacepijo z Micrococcus luteus in inkubirajo 24 ur pri 30 °C.
English: Store the culture in a refrigerator at about 4 _C.
Slovenian: Kultura se hrani v hladilniku pri 4 °C.
English: Use this suspension to inoculate 250 ml of culture medium (4.1) contained in a Roux flask and incubate for 18 to 20 hours at 30 _C.
Slovenian: S to suspenzijo se nacepi 250 ml gojišča (4.1), ki se nahaja v Rouxovi steklenici, in se inkubira od 18 do 20 ur pri 30 °C.
English: This suspension may be kept for one week at about 4 _C.
Slovenian: Suspenzija je obstojna en teden pri približno 4 °C.
English: Stored in a stoppered flask at 4 _C this solution is stable for up to five days.
Slovenian: Raztopina je stabilna do pet dni, če se hrani v zaprti posodi pri 4 °C.
English: Leave to settle or centrifuge, take 20 ml of the supernatant solution and evaporate to about 5 ml in a rotary evaporator at a temperature not exceeding 40 _C.
Slovenian: Pusti se, da se mešanica usede, ali centrifugira, nato se odpipetira 20 ml bistre raztopine nad usedlino in pusti, da izpari do približno 5 ml v rotacijskem uparjalniku pri temperaturi, ki ne presega 40 °C.
English: Inoculate the assay medium (4.1) with the bacterial suspension (3.2) at about 50 _C.
Slovenian: Preskusno gojišče (4.1) se nacepi s suspenzijo bakterij (3.2) pri približno 50 °C.
English: Incubate the plates for 16 to 18 hours at 30 _ 2 _C.
Slovenian: Plošče se inkubirajo od 16 do 18 ur pri 30 ± 2 °C.
English: Inoculate tubes containing slopes of culture medium (4.1) with Micrococcus luteus (flavus) and incubate for 24 hours at 30 _C.
Slovenian: Epruvete s poševnimi nanosi gojišča (4.1) se nacepijo z Micrococcus luteus (flavus) in inkubirajo 24 ur pri 30 °C.
English: Bromocresol purple solution 0,04 % (w/v): dissolve 0,1 g of bromocresol purple in 18,5 ml of 0,01 M sodium hydroxide solution. Make up the volume to 250 ml with water and mix. 4.11. Standard substance: zinc bacitracin of known activity (in i. u.).
Slovenian: Bromkrezol rdeče, raztopina 0,04 % (m/v): 0,1 g bromkrezol rdečega se raztopi v 18,5 ml raztopine natrijevega hidroksida 0,01 M. Dopolni se z vodo do 250 ml in premeša.
English: Take a suitable aliquot of the supernatant solution and adjust the pH to 6,5 by means of 1 M hydroxide solution (4.8) with a pH-meter or with the bromocresol purple solution (4.10) as indicator. Dilute with phosphate buffer (4.5) to obtain an expected zinc bacitracin content of 0,42 i.u./ml (=u8).
Slovenian: Odpipetira se ustrezen alikvot bistre raztopine nad usedlino in z 1 M raztopino hidroksida (4.8) se vrednost pH naravna na 6,5, pri čemer kot indikator služi bodisi pH-meter bodisi raztopina bromkrezol rdečega (4.10). Mešanica se razredči s fosfatnim pufrom (4.5), da se dobi pričakovana vsebnost cink bacitracina 0,42 i.u./ml (=u8).
English: Take a suitable volume of the supernatant solution and adjust the pH to 6,5 by means of 1 M sodium hydroxide solution (4.8) with a pH-meter or with the bromocresol purple solution (4.10) as indicator. Evaporate to approximately half volume in a rotary evaporator at a temperature not exceeding 35 _C.
Slovenian: Odpipetira se ustrezen volumen bistre raztopine nad usedlino in z dodajanjem raztopine natrijevega hidroksida 1 M (4.8) se vrednost pH naravna na 6,5, pri čemer kot indikator služi bodisi pH-meter bodisi raztopina bromkrezol rdečega (4.10). V rotacijskem uparjalniku se upari približno polovica volumna pri temperaturi, ki ne presega 35 °C.
English: Evaporate to about 4 ml in a rotary evaporator at a temperature not exceeding 35 _C.
Slovenian: V rotacijskem uparjalniku se upari do 4 ml pri temperaturi, ki ne presega 35 °C.
English: Inoculate the assay medium (4.2) with the bacterial suspension (3.2) at about 50 _C.
Slovenian: Preskusno gojišče (4.2) se nacepi s suspenzijo bakterij (3.2) pri približno 50 °C.
English: Similarly record UL and UH and join them to give the "best fit" line for the extract. In the absence of any interference the lines should be parallel.
Slovenian: Na podoben način se izračunata vrednosti UL in UH ter povežeta, da se dobi premica z "najboljšim" potekom za ekstrakt.
English: Member States shall, by 1 June 1984 at the latest, bring into force the laws, regulations or administrative provisions necessary to comply with this Directive.
Slovak: Členské štáty príjmu najneskôr do 1. júna 1984 zákony, iné právne predpisy a administratívne opatrenia potrebné na dosiahnutie súladu s touto smernicou.
English: Shall forthwith inform the Commission thereof.
Slovak: Bezodkladne o tom budú informovať Komisiu.
English: Use this suspension to inoculate 250 ml of culture medium (4.1) contained in a Roux flask and incubate for 18 to 20 hours at 30 °C. Harvest the growth in 25 ml of sodium chloride solution (4.3) and mix.
Slovak: Túto suspenziu použite na naočkovanie 250 ml kultivačného média (4.1), v Rouxovej banke a inkubujte 18 až 20 hodín pri teplote 30 C. Zozbierajte produkt do 25 ml roztoku chloridu sodného (4.3) a zamiešajte.
English: Sodium chloride solution 0,8 % (w/v):
Slovak: Chlorid sodný, roztok 0,8 % (w/v):
Romanian: Soluție de 0,8 % (p/v) clorură de sodiu:
Bulgarian: Разтвор на натриев хлорид 0,8% (w/v):
Estonian: 0,8(massi/mahu)protsendiline naatriumkloriidi lahus:
English: -dissolve 8 g sodium chloride in water and dilute to 1 000 ml;
Slovak: -rozpustite 8 g chloridu sodného vo vode a zrieďte na 1 000 ml;
English: Log A is Activity of sample extract = activity of relevant standard × A (u8 = s8 × A)
Slovak: Log A =Aktivita skúšobného extraktu = aktivita príslušného štandardu x A (u8 = s8 x A)
English: Activity of sample extract
Slovak: Aktivita skúšobného extraktu
English: = activity of relevant standard
Slovak: = aktivita príslušného štandardu
English: Culture and assay medium
Romanian: Mediul de întreținere a sușei și de bază a dozării
Estonian: Sööde ja määramissöödeb
English: Meat peptone6,0 g
Estonian: Lihapeptoon 6,0 g
English: dissolve 8 g sodium chloride in water and dilute to 1 000 ml;
Bulgarian: разтварят се 8 г натриев хлорид във вода и се разрежда до 1 000 мл;
Estonian: 8 g naatriumkloriidi lahustatakse vees ja lahjendatakse 1 000 milliliitrini;
English: If the lines are found to be non-parallel either u1 and s1 or u8 and s8 may be discarded and SL, SH, UL and UH calculated, using the alternative formulae, to give alternative "best fit" lines: and similarly for UL and UH.
Estonian: Kui jooned ei ole paralleelsed, siis võib visata välja kas u1 ja s1 või u8 ja s8 ning arvutada SL, SH, UL ja UH järgmiste valemite abil, et saada alternatiivsed "kõige sobivamad" jooned: ning samamoodi UL ja UH puhul.
Latvian: Konstatējot, ka līnijas nav paralēlas, var atmest u1 un s1 vai u8 un s8, un SL, SH, UL un UH "piemērotākās" līknes aprēķināt pēc alternatīvām formulām: un līdzīgi aprēķina UL un UH.
English: Micro-organism:
Romanian: Microorganismul :
Bulgarian: Микроорганизъм:
Estonian: Mikroorganism:
Latvian: Mikroorganisms:
Maltese: Mikro-organiżmu:
English: Micrococcus luteus (flavus) ATCC 10240
Romanian: Micrococcus luteus (flavus) ATCC 10240
Bulgarian: Micrococcus luteus АТСС 10240
Estonian: Micrococcus luteus (flavus) ATCC 10240
Latvian: Micrococcus luteus (flavus) ATCC 10240
English: From this solution prepare by successive dilution with phosphate buffer (4.5) the following solutions: s8 0,42 i.u./ml s4 0,21 i.u./ml s2 0,105 i.u./ml s1 0,0525 i.u./ml
Estonian: Kõnealusest lahusest valmistatakse fosfaatpuhvriga (4.5) järjest lahjendades järgmised lahused: s8 0,42 RÜ/ml s4 0,21 RÜ/ml s2 0,105 RÜ/ml s1 0,0525 RÜ/ml
English: // Meat peptone // 6,0 g // Tryptone // 4,0 g // Yeast extract // 3,0 g // Meat extract // 1,5 g // Glucose // 1,0 g // Agar // 10,0 to 20,0 g // Water // 1 000 ml // ph 6,5 (after sterilization). //
French: // Peptone de viande // 6,0 g // Tryptone // 4,0 g // Extrait de levure // 3,0 g // Extrait de viande // 1,5 g // Glucose // 1,0 g // Gélose // 10,0 à 20,0 g // Eau pH 6,5 (après stérilisation) // 1 000 ml
English: To this effect, select plates big enough to allow at least eight holes with a diameter of 10 to 13 mm and not less than 30 mm between centres to be made in the agar medium. The test may be carried out on plates consisting of a sheet of glass with a faced aluminium or plastic ring placed on top, 200 mm in diameter and 20 mm high.
French: À cet effet, choisir la dimension des boîtes de telle sorte qu'on puisse creuser dans le milieu gélosé au moins huit cavités de 10 à 13 mm de diamètre, dont les centres ne sont pas distants de moins de 30 mm. On peut utiliser comme boîtes des plaques de verre planes, surmontées d'un anneau d'aluminium ou de matière plastique de 200 mm de diamètre et 20 mm de hauteur.
English: // Meat peptone // 6,0 g // Tryptone // 4,0 g // Yeast extract // 3,0 g // Meat extract // 1,5 g // Glucose // 1,0 g // Agar // 10,0 to 20,0 g // Water // 1 000 ml // pH 6,5 to 6,6 (after sterilization). //
French: // Peptone de viande // 6,0 // g // Tryptone // 4,0 // g // Extrait de levure // 3,0 // g // Extrait de viande // 1,5 // g // Glucose // 1,0 // g // Gélose // 10,0 à 20,0 // g // Eau // 1 000 // ml // pH 6,5-6,6 (après stérilisation) // //
English: // Tryptone // 10,0 g // Yeast extract // 3,0 g // Meat extract // 1,5 g // Glucose // 1,0 g // Agar // 10,0 to 20,0 g // Tween 80 // 1 ml // Water // 1 000 ml // pH 6,5 (after sterilization). //
French: // Tryptone // 10,0 // g // Extrait de levure // 3,0 // g // Extrait de viande // 1,5 // g // Glucose // 1,0 // g // Gélose // 10,0 à 20,0 // g // Tween 80 // 1 // ml // Eau // 1 000 // ml // pH 6,5 (après stérilisation) // //
English: STANDARD SUBSTANCE : ZINC BACITRACIN OF KNOWN ACTIVITY (IN I. U.).
French: SUBSTANCE ETALON : BACITRACINE-ZINC D'ACTIVITE CONNUE (EN UI).
English: STANDARD SOLUTIONS
French: SOLUTIONS ETALONS
English: WEIGH OUT A QUANTITY OF STANDARD ZINC BACITRACIN (4.11) CORRESPONDING TO 1 050 I.U. (ACCORDING TO THE ACTIVITY INDICATED).
French: PESER UNE QUANTITE DE SUBSTANCE ETALON (4.11) CORRESPONDANT A 1 050 UI (SELON LE TITRE INDIQUE).
English: ADD 5 ML OF 0,1 M HYDROCHLORIC ACID (4.7) AND LEAVE TO STAND FOR 15 MINUTES.
French: AJOUTER 5 ML D'ACIDE CHLORHYDRIQUE 0,1 M (4.7) ET LAISSER REPOSER QUINZE MINUTES.
English: ADD 30 ML OF WATER, ADJUST THE PH TO 4,5 WITH PHOSPHATE BUFFER (4.5) (ABOUT 4 ML), MAKE UP TO A VOLUME OF 50 ML WITH WATER AND MIX WELL (1 ML = 21 I.U.).
French: AJOUTER 30 ML D'EAU, AJUSTER LE PH A 4,5 A L'AIDE DU TAMPON PHOSPHATE (4.5) (4 ML ENVIRON), COMPLETER A 50 ML AVEC DE L'EAU ET HOMOGENEISER (1 ML = 21 UI).
English: FROM THIS SOLUTION PREPARE BY SUCCESSIVE DILUTION WITH PHOSPHATE BUFFER (4.5) THE FOLLOWING SOLUTIONS :
French: PREPARER A PARTIR DE CETTE SOLUTION ET PAR DILUTIONS SUCCESSIVES A L'AIDE DU TAMPON PHOSPHATE PH 6,5 (4.5) LES SOLUTIONS SUIVANTES :
English: PREPARATION OF THE EXTRACT AND ASSAY SOLUTIONS
French: PREPARATION DE L'EXTRAIT
English: EXTRACTION
French: EXTRACTION
English: PREMIXES AND MINERAL FEEDS
French: PREMELANGES ET ALIMENTS MINERAUX
English: WEIGH OUT A QUANTITY OF SAMPLE OF 2,0 TO 5,0 G, ADD 29,0 ML OF THE MIXTURE (4.4) AND 1,0 ML OF SODIUM SULPHIDE SOLUTION (4.9) AND SHAKE BRIEFLY.
French: PESER UNE QUANTITE D'ECHANTILLON DE 2,0 A 5,0 G, AJOUTER 29,0 ML DU MELANGE (4.4) ET 1,0 ML DE SOLUTION DE SULFURE DE SODIUM (4.9); AGITER BRIEVEMENT.
English: CHECK THAT THE PH IS ABOUT 2.
French: VERIFIER QUE LE PH EST DE 2 ENVIRON.
English: SHAKE FOR 10 MINUTES, ADD 30 ML OF PHOSPHATE BUFFER (4.5), SHAKE FOR 15 MINUTES AND CENTRIFUGE.
French: AGITER DURANT DIX MINUTES, AJOUTER 30 ML DE TAMPON PHOSPHATE (4.5), AGITER DURANT QUINZE MINUTES ET CENTRIFUGER.
English: TAKE A SUITABLE ALIQUOT OF THE SUPERNATANT SOLUTION AND ADJUST THE PH TO 6,5 BY MEANS OF 1 M HYDROXIDE SOLUTION (4.8) WITH A PH-METER OR WITH THE BROMOCRESOL PURPLE SOLUTION (4.10) AS INDICATOR.
French: PRELEVER UNE PARTIE ALIQUOTE DE LA SOLUTION SURNAGEANTE ET AMENER LE PH A 6,5 A L'AIDE DE LA SOLUTION 1 M D'HYDROXYDE DE SODIUM (4.8) EN UTILISANT UN PH-METRE OU LA SOLUTION DE POURPRE DE BROMOCRESOL (4.10) COMME INDICATEUR.
English: DILUTE WITH PHOSPHATE BUFFER (4.5) TO OBTAIN AN EXPECTED ZINC BACITRACIN CONTENT OF 0,42 I.U./ML (= U8).
French: DILUER A L'AIDE DU TAMPON PHOSPHATE (4.5) POUR OBTENIR UNE CONCENTRATION PRESUMEE EN BACITRACINE-ZINC DE 0,42 UI/ML (= U8).
English: PROTEIN CONCENTRATES
French: CONCENTRES PROTEINIQUES
English: WEIGH OUT A QUANTITY OF SAMPLE OF 10,0 G, ADD 49,0 ML OF THE MIXTURE (4.4) AND 1,0 ML OF SODIUM SULPHIDE SOLUTION (4.9) AND SHAKE BRIEFLY.
French: PESER UNE QUANTITE D'ECHANTILLON DE 10,0 G, AJOUTER 49,0 ML DU MELANGE (4.4) ET 1,0 ML DE LA SOLUTION DE SULFURE DE SODIUM (4.9); AGITER BRIEVEMENT.
English: CHECK THAT THE PH IS ABOUT 2. SHAKE FOR 10 MINUTES.
French: VERIFIER QUE LE PH EST DE 2 ENVIRON.
English: ADD 50 ML OF PHOSPHATE BUFFER (4.5), SHAKE FOR 15 MINUTES AND CENTRIFUGE.
French: AGITER DURANT DIX MINUTES, AJOUTER 50 ML DU TAMPON PHOSPHATE (4.5), AGITER DURANT QUINZE MINUTES ET CENTRIFUGER.
English: TAKE A SUITABLE VOLUME OF THE SUPERNATANT SOLUTION AND ADJUST THE PH TO 6,5 BY MEANS OF 1 M SODIUM HYDROXIDE SOLUTION (4.8) WITH A PH-METER OR WITH THE BROMOCRESOL PURPLE SOLUTION (4.10) AS INDICATOR.
French: PRELEVER UNE PARTIE ALIQUOTE DE LA SOLUTION SURNAGEANTE ET AMENER LE PH A 6,5 A L'AIDE DE LA SOLUTION 1 M D'HYDROXYDE DE SODIUM (4.8) EN UTILISANT UN PH-METRE OU LA SOLUTION DE POURPRE DE BROMOCRESOL (4.10) COMME INDICATEUR.
English: EVAPORATE TO APPROXIMATELY HALF VOLUME IN A ROTARY EVAPORATOR AT A TEMPERATURE NOT EXCEEDING 35 C. DILUTE WITH PHOSPHATE BUFFER (4.5) TO OBTAIN AN EXPECTED ZINC BACITRACIN CONTENT OF 0,42 I.U./ML (= U8).
French: EVAPORER APPROXIMATIVEMENT LA MOITIE DU VOLUME DANS UN EVAPORATEUR ROTATIF A UNE TEMPERATURE NE DEPASSANT PAS 35 C. DILUER A L'AIDE DU TAMPON PHOSPHATE (4.5) POUR OBTENIR UNE CONCENTRATION PRESUMEE EN BACITRACINE-ZINC DE 0,42 UI/ML (= U8).
English: OTHER FEEDS
French: AUTRES ALIMENTS
English: WEIGH OUT A QUANTITY OF SAMPLE OF 10,0 G (20,0 G FOR AN EXPECTED ZINC BACITRACIN CONTENT OF 5 MG/KG).
French: PESER UNE QUANTITE D'ECHANTILLON DE 10,0 G (20,0 G POUR UNE CONCENTRATION PRESUMEE EN BACITRACINE-ZINC ZINC DE 5 MG/KG).
English: ADD A MIXTURE OF 24,0 ML OF THE MIXTURE (4.4) AND 1,0 ML OF SODIUM SULPHIDE SOLUTION (4.9) AND HOMOGENIZE FOR 10 MINUTES.
French: AJOUTER 24,0 ML DU MELANGE (4.4) ET 1,0 ML DE LA SOLUTION DE SULFURE DE SODIUM (4.9); HOMOGENEISER DURANT DIX MINUTES.
English: ADD 25 ML OF PHOSPHATE BUFFER (4,5), SHAKE FOR 15 MINUTES AND CENTRIFUGE.
French: AJOUTER 25 ML DE TAMPON PHOSPHATE (4.5), AGITER DURANT QUINZE MINUTES ET CENTRIFUGER.
English: TAKE 20 ML OF THE SUPERNATANT SOLUTION AND ADJUST THE PH TO 6.5 BY MEANS OF 1 M SODIUM HYDROXIDE SOLUTION (4.8) WITH A PH-METER OR WITH THE BROMOCRESOL PURPLE SOLUTION (4.10) AS INDICATOR.
French: PRELEVER 20 ML DE LA SOLUTION SURNAGEANTE ET AJUSTER LE PH A 6,5 A L'AIDE DE LA SOLUTION 1 M D'HYDROXYDE DE SODIUM (4.8) EN UTILISANT UN PH-METRE OU LA SOLUTION DE POURPRE DE BROMOCRESOL (4.10) COMME INDICATEUR.
English: EVAPORATE TO ABOUT 4 ML IN A ROTARY EVAPORATOR AT A TEMPERATURE NOT EXCEEDING 35 C. DILUTE THE RESIDUE WITH PHOSPHATE BUFFER (4.5) TO OBTAIN AN EXPECTED ZINC BACITRACIN CONTENT OF 0,42 I.U./ML (= U8).
French: EVAPORER JUSQU'A 4 ML ENVIRON DANS UN EVAPORATEUR ROTATIF A UNE TEMPERATURE NE DEPASSANT PAS 35 C. DILUER LE RESIDU A L'AIDE DU TAMPON PHOSPHATE (4.5) POUR OBTENIR UNE CONCENTRATION PRESUMEE EN BACITRACINE-ZINC DE 0,42 UI/ML (= U8).
English: ASSAY SOLUTIONS
French: SOLUTIONS DE L'EXTRAIT
English: FROM SOLUTION U8 PREPARE SOLUTIONS U4 (EXPECTED CONTENT : 0,21 I.U./ML), U2 (EXPECTED CONTENT : 0,105 I.U./ML) AND U1 (EXPECTED CONTENT : 0,0525 I.U./ML) BY MEANS OF SUCCESSIVE DILUTION (1 + 1) WITH PHOSPHATE BUFFER (4.5).
French: PREPARER A PARTIR DE LA SOLUTION U8 ET PAR DILUTIONS SUCCESSIVES (1 + 1) A L'AIDE DU TAMPON PHOSPHATE (4.5), LES SOLUTIONS U4 (CONCENTRATION PRESUMEE : 0,21 UI/ML), U2 (CONCENTRATION PRESUMEE : 0,105 UI/ML) ET U1 (CONCENTRATION PRESUMEE : 0,0525 UI/ML).
English: ASSAY PROCEDURE
French: MODALITES DU DOSAGE
English: INOCULATION OF THE ASSAY MEDIUM
French: INOCULATION DU MILIEU DE CULTURE
English: INOCULATE THE ASSAY MEDIUM (4.2) WITH THE BACTERIAL SUSPENSION (3.2) AT ABOUT 50 C. BY PRELIMINARY TRIALS ON PLATES WITH ASSAY MEDIUM (4.2) DETERMINE THE QUANTITY OF BACTERIAL SUSPENSION REQUIRED TO GIVE THE LARGEST AND CLEAREST ZONES OF INHIBITION WITH THE VARIOUS CONCENTRATIONS OF ZINC BACITRACIN.
French: ENSEMENCER A 50 C ENVIRON LE MILIEU DE BASE DU DOSAGE (4.2) PAR LA SUSPENSION BACTERIENNE (3.2). PAR DES ESSAIS PRELIMINAIRES SUR PLAQUES AVEC LE MILIEU (4.2), DETERMINER LA QUANTITE DE SUSPENSION BACTERIENNE PERMETTANT D'OBTENIR POUR LES DIFFERENTES CONCENTRATIONS EN BACITRACINE-ZINC DES ZONES D'INHIBITION AUSSI ETENDUES QUE POSSIBLE ET QUI SOIENT ENCORE NETTES.
English: PREPARATION OF THE PLATES
French: PREPARATION DES BOITES
English: DIFFUSION THROUGH AGAR IS CARRIED OUT IN PLATES WITH THE FOUR CONCENTRATIONS OF THE STANDARD SOLUTION (S8, S4, S2 AND S1) AND THE FOUR CONCENTRATIONS OF THE ASSAY SOLUTION (U8, U4, U2 AND U1).
French: LA DIFFUSION SUR GELOSE S'EFFECTUE DANS DES BOITES AVEC LES QUATRE CONCENTRATIONS DE LA SOLUTION ETALON (S8, S4, S2, S1) ET LES QUATRE CONCENTRATIONS DE L'EXTRAIT (U8, U4, U2, U1).
English: THESE FOUR CONCENTRATIONS OF EXTRACT AND STANDARD MUST NECESSARILY BE PLACED IN EACH PLATE.
French: CHAQUE BOITE DOIT NECESSAIREMENT RECEVOIR LES QUATRE CONCENTRATIONS DE L'ETALON ET DE L'EXTRAIT.
English: TO THIS EFFECT, SELECT PLATES BIG ENOUGH TO ALLOW AT LEAST EIGHT HOLES WITH A DIAMETER OF 10 TO 13 MM AND NOT LESS THAN 30 MM BETWEEN CENTRES TO BE MADE IN THE AGAR MEDIUM. THE TEST MAY BE CARRIED OUT ON PLATES CONSISTING OF A SHEET OF GLASS WITH A FACED ALUMINIUM OR PLASTIC RING PLACED ON TOP, 200 MM IN DIAMETER AND 20 MM HIGH.
French: A CET EFFET, CHOISIR LA DIMENSION DES BOITES DE TELLE SORTE QU'ON PUISSE CREUSER DANS LE MILIEU GELOSE AU MOINS HUIT CAVITES DE 10 A 13 MM DE DIAMETRE, DONT LES CENTRES NE SONT PAS DISTANTS DE MOINS DE 30 MM. ON PEUT UTILISER COMME BOITES DES PLAQUES DE VERRE PLANES, SURMONTEES D'UN ANNEAU D'ALUMINIUM OU DE MATIERE PLASTIQUE DE 200 MM DE DIAMETRE ET 20 MM DE HAUTEUR.
English: POUR INTO THE PLATES A QUANTITY OF THE MEDIUM (4.2) INOCULATED AS IN POINT 7.1, TO GIVE A LAYER ABOUT 2 MM THICK (60 ML FOR A PLATE OF 200 MM DIAMETER), ALLOW TO SET IN A LEVEL POSITION, BORE THE HOLES AND PLACE IN THEM EXACTLY MEASURED VOLUMES OF ASSAY AND STANDARD SOLUTIONS (BETWEEN 0,10 AND 0,15 ML PER HOLE, ACCORDING TO THE DIAMETER).
French: INTRODUIRE DANS LES BOITES UNE QUANTITE DU MILIEU (4.2), ENSEMENCE COMME INDIQUE EN 7.1, PERMETTANT D'OBTENIR UNE COUCHE DE 2 MM ENVIRON D'EPAISSEUR (60 ML POUR UNE BOITE DE 200 MM DE DIAMETRE). LAISSER SOLIDIFIER, CREUSER LES CAVITES ET Y DEPOSER DES VOLUMES EXACTEMENT MESURES DES SOLUTIONS DE L'ETALON ET DE L'EXTRAIT (0,10 A 0,15 ML PAR CAVITE, SELON LE DIAMETRE).
English: APPLY EACH CONCENTRATION AT LEAST FOUR TIMES SO THAT EACH DETERMINATION IS SUBJECT TO AN EVALUATION OF 32 ZONES OF INHIBITION.
French: FAIRE AU MOINS QUATRE REPETITIONS DE CHAQUE CONCENTRATION DE FACON QUE CHAQUE DETERMINATION FASSE L'OBJET D'UNE EVALUATION DE 32 ZONES D'INHIBITION.
English: INCUBATION
French: INCUBATION