FrancophonIA/DGT-Translation_Memory huggingface dataset:
English: DILUTION IS CARRIED OUT USING THE PHOSPHATE BUFFER SOLUTION, PH 5.5, DILUTED TO 1/10, TO WHICH 0.01 % OF AMIDO BLACK HAS BEEN ADDED.
Slovenian: Razredčujemo jo z raztopino fosfatnega puferja z vrednostjo pH 5,5, ki je razredčena na 1/10 in vsebuje dodatek 0,01 % amido črne.
English: THEN PREPARE BY SUCCESSIVE DILUTIONS (1 + 1), USING THE BUFFER SOLUTION, THE FOLLOWING CONCENTRATIONS:
Slovenian: Z dodatnim razredčevanjem (1 + 1) s pufrsko raztopino pripravimo nato naslednje koncentracije:
English: PROCEEDING AS INDICATED IN 5.1, PREPARE, FROM A STOCK SOLUTION WITH A CONCENTRATION CORRESPONDING TO 400 UG PER ML OF OXYTETRACYCLINE-HCL, A STANDARD WORKING SOLUTION S8 CONTAINING 1.6 UG PER ML OF OXYTETRACYCLINE-HCL, AND THE FOLLOWING CONCENTRATIONS:
Slovenian: Po navodilih iz 5.1 pripravimo iz osnovne raztopine s koncentracijo, ki ustreza 400 g na ml oksitetraciklina-HCl, standardno delovno raztopino S8, ki vsebuje 1,6 g na ml oksitetraciklina-HCl, in naslednje koncentracije:
English: PROCEEDING AS INDICATED IN 5.1, PREPARE, FROM A STOCK SOLUTION WITH A CONCENTRATION CORRESPONDING TO 500 UG PER ML OF TETRACYCLINE-HCL, A STANDARD WORKING SOLUTION S8 CONTAINING 1.0 UG PER ML OF TETRACYCLINE-HCL AND THE FOLLOWING CONCENTRATIONS:
Slovenian: Po navodilih iz 5.1 pripravimo iz osnovne raztopine s koncentracijo, ki ustreza 500 g na ml tetraciklina-HCl, standardno delovno raztopino S8, ki vsebuje 1,0 g na ml tetraciklina-HCl, in naslednje koncentracije:
English: TO TEST SAMPLE ADD FORMAMIDE IN THE QUANTITIES INDICATED IN THE TABLE BELOW.
Slovenian: Za preskus vzorca dodamo formamid, in to v količinah, ki so navedene v tabeli spodaj.
English: THEN DILUTE IMMEDIATELY WITH THE PHOSPHATE BUFFER SOLUTION ACCORDING TO THE INDICATIONS GIVEN IN THE TABLE BELOW TO OBTAIN THE CONCENTRATION U8.
Slovenian: Ob upoštevanju navedb v tabeli spodaj ga nato nemudoma razredčimo z raztopino fosfatnega puferja, da dobimo koncentracijo U8.
English: THEN PREPARE THE CONCENTRATIONS U4, U2 AND U1 BY SUCCESSIVE DILUTIONS (1 + 1) USING THE PHOSPHATE BUFFER SOLUTION.
Slovenian: Nato z zaporednim razredčevanjem (1 + 1) z raztopino fosfatnega puferja pripravimo koncentracije U4, U2 in U1.
English: TO A TEST SAMPLE OF 2 TO 10 G, DEPENDING ON THE PRESUMED ANTIBIOTIC CONTENT OF THE SAMPLE OR ITS MANUFACTURER'S GUARANTEE, ADD 20 TIMES ITS VOLUME OF MIXTURE.
Slovenian: Preskusnemu vzorcu od 2 do 10 g dodamo 20-krat večjo količino mešanice, odvisno od pričakovane antibiotične vsebnosti vzorca ali proizvajalčeve garancije.
English: IF NECESSARY, READJUST TO PH 3 (USING 10 % ACETIC ACID FOR MINERAL COMPOUNDS).
Slovenian: Po potrebi jo spet naravnamo na 3 (pri mineralnih spojinah z 10-odstotno ocetno kislino).
English: TAKE AN ALIQUOT PART OF THE EXTRACT AND ADJUST THE PH TO 5.5 USING THE PHOSPHATE BUFFER SOLUTION, PH 8 IN THE PRESENCE OF BROMOCRESOL GREEN (TURNING FROM YELLOW TO BLUE).
Slovenian: Vzamemo alikvotni delež ekstrakta in z raztopino fosfatnega puferja, pH 8, z dodatkom bromkrezol zelene naravnamo njegovo pH vrednost na 5,5 (prehod z rumene na modro).
English: DILUTE, USING THE PHOSPHATE BUFFER SOLUTION, PH 5.5, DILUTED TO 1/10, TO OBTAIN THE CONCENTRATION U8 (SEE 6.1).
Slovenian: Spojino razredčimo z raztopino fosfatnega puferja, pH 5,5, ki je razredčena na 1/10, da dobimo koncentracijo U8 (glej 6.1).
English: DIFFUSION ON AGAR IS CARRIED OUT IN TRAYS USING 4 CONCENTRATIONS OF THE STANDARD SOLUTION (S8, S4, S2, S1) AND 4 CONCENTRATIONS OF THE EXTRACT (U8, U4, U2, U1).
Slovenian: Difuzija na agarju se izvaja na ploščah s štirimi koncentracijami standardne raztopine (S8, S4, S2, S1) in s štirimi koncentracijami ekstrakta (U8, U4, U2, U1).
English: CHOOSE TRAYS, THEREFORE, WHICH ARE LARGE ENOUGH TO ALLOW AT LEAST 8 HOLES 10 TO 13 MM IN DIAMETER TO BE MADE IN THE AGAR MEDIUM.
Slovenian: Zato izberemo plošče, ki so dovolj velike, da v agarsko gojišče lahko vdolbemo vsaj 8 vdolbin premera od 10 do 13 mm.
English: CALCULATE THE QUANTITY OF INOCULATED CULTURE MEDIUM NEEDED TO PROVIDE A UNIFORM COVERING APPROXIMATELY 2 MM THICK.
Slovenian: Izračunamo količino nacepljenega gojišča, ki je potrebna, da dobimo enakomerno prevleko s približno 2 mm debelino.
English: THE TEST SHOULD PREFERABLY BE CARRIED OUT ON TRAYS CONSISTING OF FLAT GLASS PLATES FITTED WITH A PERFECTLY LEVEL ALUMINIUM OR PLASTIC RING, 200 MM IN DIAMETER AND 20 MM HIGH.
Slovenian: Najbolje, da se preskus izvaja na ploščah s plosko stekleno površino, ki imajo popolnoma raven aluminijev ali plastični obroč, premera 200 mm in višine 20 mm.
English: FOR EACH SAMPLE, REPEAT THE DIFFUSION AT LEAST 4 TIMES WITH EACH CONCENTRATION SO THAT EACH DETERMINATION COMPRISES AN EVALUATION OF 32 INHIBITION ZONES.
Slovenian: Za vsak vzorec ponovimo difuzijo vsaj štirikrat z vsako koncentracijo, tako da pri vsakem določanju ovrednotimo 32 inhibicijskih con.
English: MEASURE THE DIAMETER OF THE INHIBITION ZONES, PREFERABLY BY PROJECTION.
Slovenian: Izmerimo premer inhibicijskih con, najraje s projekcijo.
English: RECORD THE MEASUREMENTS ON SEMI-LOGARITHMIC PAPER, PLOTTING THE LOGARITHM OF THE CONCENTRATIONS AGAINST THE DIAMETER OF THE INHIBITION ZONES.
Slovenian: Meritve zabeležimo na semilogaritemski papir in jih prikažemo kot logaritem koncentracij proti premeru inhibicijskih con.
English: PROVIDED THERE IS NO INTERFERENCE, THE TWO LINES WILL BE PARALLEL.
Slovenian: Če ni interferenc, bosta črti vzporedni.
English: THE LOGARITHM OF THE RELATIVE ACTIVITY IS CALCULATED BY USING THE FOLLOWING FORMULA:
Slovenian: Logaritem relativne aktivnosti se izračuna z naslednjo enačbo:
English: THE DIFFERENCE BETWEEN THE RESULTS OF TWO PARALLEL DETERMINATIONS CARRIED OUT ON THE SAME SAMPLE MUST NOT EXCEED 10 %, IN RELATIVE VALUE.
Slovenian: Relativna vrednost razlike med rezultati dveh vzporednih določanj, izvedenih na istem vzorcu, ne sme presegati 10 %.
English: THIS METHOD MAKES IT POSSIBLE TO DETERMINE THE LEVELS OF CHLORTETRACYCLINE (CTC), OXYTETRACYCLINE (OTC) AND TETRACYCLINE (TC) WHERE CONCENTRATIONS ARE GREATER THAN 1 G PER KG, PROVIDED THERE IS NO INTERFERENCE FROM OTHER SUBSTANCES CLOUDING THE EXTRACTS.
Slovenian: S to metodo je mogoče določiti ravni klortetraciklina (CTC), oksitetraciklina (OT) in tetraciklina (TC), kjer so koncentracije večje od 1 g na kg, če ekstrakti niso motni zaradi drugih snovi.
English: FOR THE DETERMINATION OF CTC, THE SAMPLE IS EXTRACTED WITH A MIXTURE OF ACETONE, WATER AND HYDROCHLORIC ACID, AND WITH A MIXTUE OF METHANOL AND HYDROCHLORIC ACID FOR THE DETERMINATION OF OTC AND TC.
Slovenian: Kadar določamo CTC, izlužimo vzorec z mešanico acetona, vode in klorovodikove kisline, kadar določamo OTC in TC, pa z mešanico metanola in klorovodikove kisline.
English: STAPHYLOCOCCUS AUREUS K 141
Slovenian: Staphylococcus aureus K 141
English: INOCULATE WITH S. AUREUS A TUBE OF SLOPED AGAR TAKEN FROM THE CULTURE MEDIUM, TO WHICH 1.5 TO 3 % OF AGAR HAS BEEN ADDED (DEPENDING ON THE QUALITY).
Slovenian: V epruveto s poševnim nanosom agarja z gojišča, kateremu smo dodali od 1,5 do 3 % agarja (odvisno od kakovosti), nacepimo S. aureus.
English: 24 HOURS BEFORE USE, RE-INOCULATE SLOPED AGAR WITH A SUB-CULTURE AND INCUBATE OVERNIGHT AT 37* C.
Slovenian: 24 ur pred uporabo ponovno nacepimo nanos agarja s subkulturo in pustimo, da čez noč poteka inkubacija pri 37°C.
English: SUSPEND ALL THE CULTURE CONTAINED IN A TUBE OF AGAR IN APPROXIMATELY 2 ML OF THE BASIC MEDIUM, THEN TRANSFER THE SUSPENSION UNDER STERILE CONDITIONS INTO APPROXIMATELY 100 ML OF THE SAME BASIC MEDIUM.
Slovenian: Vso kulturo v epruveti z agarjem suspendiramo v približno 2 ml osnovnega gojišča, nato prenesemo suspenzijo na sterilni način v približno 100 ml istega osnovnega gojišča.
English: PH AFTER STERILIZATION: 6.8 TO 7.0.
Slovenian: pH po sterilizaciji: od 6,8 do 7,0.
English: MIXTURE OF PURE ACETONE/WATER/HYDROCHLORIC ACID (D: 1.19) : 65/33/2 BY VOLUME.
Slovenian: Mešanica čistega acetona/vode/klorovodikove kisline (d: 1,19):65/33/2 po prostornini.
English: USING HYDROCHLORIC ACID, PREPARE FROM THE STANDARD SUBSTANCE A STOCK SOLUTION WITH A CONCENTRATION CORRESPONDING TO 400 TO 500 UG PER ML OF CTC-HCL, OTC-HCL OR TC-HCL.
Slovenian: S klorovodikovo kislino pripravimo iz standardne raztopine osnovno raztopino s koncentracijo, ki ustreza od 400 do 500 g na ml CTC-HCl, OTC-HCl ali TC-HCl.
English: ADD APPROXIMATELY 100 ML OF THE MIXTURE AND SHAKE FOR 30 MINUTES ON A SHAKING PLATFORM.
Slovenian: Dodamo približno 100 ml mešanice in stresamo 30 minut na stresalni plošči.
English: MAKE UP TO VOLUME WITH THE PHOSPHATE BUFFER SOLUTION, PH 4.5.
Slovenian: Prilijemo toliko raztopine fosfatnega puferja, pH 4,5, da sega tekočina do oznake.
English: DILUTE THE STANDARD SOLUTION AND THE EXTRACT WITH THE PHOSPHATE BUFFER SOLUTION, PH 4.5, TO OBTAIN A SERIES OF CONCENTRATIONS.
Slovenian: Standardno raztopino in ekstrakt razredčimo z raztopino fosfatnega puferja, pH 4,5, da dobimo serijo koncentracij.
English: FOR EACH DETERMINATION, A CALIBRATION CURVE IS DRAWN FROM THE RESPECTIVE CONCENTRATION, PERMITTING THE INTERPOLATION OF AT LEAST TWO VALUES RELATING TO THE EXTRACT.
Slovenian: Za vsako določanje izdelamo iz določene koncentracije umeritveno krivuljo, ki omogoča interpolacijo vsaj dveh vrednosti ekstrakta.
English: THE DILUTIONS SHOULD BE CHOSEN ACCORDING TO THE CONDITIONS UNDER WHICH THE STRAIN IS GROWN, WHICH MAY VARY FROM ONE LABORATORY TO ANOTHER.
Slovenian: Razredčene raztopine izberemo na podlagi pogojev, v katerih raste kultura. Ti pogoji so v posameznih laboratorijih lahko različni.
English: THE PROCEDURE IS GENERALLY THE FOLLOWING:
Slovenian: Postopek je navadno naslednji:
English: DILUTE THE STANDARD SOLUTION WITH THE PHOSPHATE BUFFER SOLUTION TO OBTAIN A STANDARD WORKING SOLUTION WITH A CONCENTRATION CORRESPONDING TO 0.2 UG PER ML OF CTC-HCL.
Slovenian: Standardno raztopino razredčimo z raztopino fosfatnega puferja, da dobimo standardno delovno raztopino s koncentracijo, ki ustreza 0,2 g na ml CTC-HCl.
English: THEN, USING THE PHOSPHATE BUFFER SOLUTION, PREPARE IN TEST TUBES, AS INDICATED BELOW, 6 DILUTIONS, EACH DILUTION IN DUPLICATE.
Slovenian: Z raztopino fosfatnega puferja pripravimo nato (kakor je navedeno spodaj) v epruvetah 6 razredčenih raztopin, vsako v dvojni izvedbi.
English: DILUTE THE EXTRACT WITH THE PHOSPHATE BUFFER SOLUTION TO OBTAIN A PRESUMED CTC-HCL CONCENTRATION OF 0.12 UG PER ML.
Slovenian: Ekstrakt razredčimo z raztopino fosfatnega puferja, da dobimo pričakovano koncentracijo CTC-HCl 0,12 g na ml.
English: PLACE 1 ML OF THIS SOLUTION IN EACH OF 2 TUBES, AND 0.75 ML (= 0.09 UG) IN EACH OF 2 OTHER TUBES.
Slovenian: Po 1 ml te raztopine prenesemo v dve epruveti, v drugi dve pa po 0,75 ml (= 0,09 g).
English: MAKE THE VOLUME OF THE LATTER TWO TUBES UP TO 1 ML WITH THE PHOSPHATE BUFFER SOLUTION.
Slovenian: Zadnji dve epruveti dopolnimo do oznake 1 ml z raztopino fosfatnega puferja.
English: DILUTE THE STANDARD SOLUTION WITH THE PHOSPHATE BUFFER SOLUTION TO OBTAIN A STANDARD WORKING SOLUTION WITH A CONCENTRATION CORRESPONDING TO 0.6 UG PER ML OF OTC-HCL OR OF TC-HCL.
Slovenian: Standardno raztopino razredčimo z raztopino fosfatnega puferja, da dobimo standardno delovno raztopino s koncentracijo, ki ustreza 0,6 g na ml OTC-HCl ali TC-HCl.
English: THEN, USING THE PHOSPHATE BUFFER SOLUTION, PREPARE IN TEST TUBES, AS INDICATED BELOW, 7 DILUTIONS, EACH DILUTION IN DUPLICATE.
Slovenian: Z raztopino fosfatnega puferja (4.2) pripravimo nato (kakor je navedeno spodaj) v epruvetah 7 razredčenih raztopin, vsako v dvojni izvedbi.
English: DILUTE THE EXTRACT WITH THE PHOSPHATE BUFFER SOLUTION TO OBTAIN A PRESUMED OTC-HCL OR TC-HCL CONCENTRATION OF 0.48 UG PER ML.
Slovenian: Ekstrakt razredčimo z raztopino fosfatnega puferja, da dobimo pričakovano koncentracijo OTC-HCl ali TC-HCl 0,48 g na ml.
English: PLACE 1 ML OF THIS SOLUTION IN EACH OF 2 TUBES, AND 0.5 ML (= 0.24 UG) IN EACH OF 2 OTHER TUBES.
Slovenian: Po 1 ml te raztopine prenesemo v dve epruveti, v drugi dve pa po 0,5 ml (= 0,24 g).
English: MAKES THE VOLUME OF THE LATTER TWO TUBES UP TO 1 ML WITH THE PHOSPHATE BUFFER SOLUTION.
Slovenian: Zadnji dve epruveti dopolnimo do oznake 1 ml z raztopino fosfatnega puferja.
English: INOCULATE THE BASIC MEDIUM FOR THE DETERMINATION WITH THE INOCULUM TO OBTAIN WITH THE PHOTOMETER AT 590 NM 85 % LIGHT TRANSMISSION IN A 5 CM CELL OR 92 % TRANSMISSION IN A 2 CM CELL, THE APPARATUS BEING SET AT 100 % TRANSMISSION ON THE NON-INOCULATED BASIC MEDIUM.
Slovenian: Za določanje nacepimo osnovno gojišče z nacepljenjem, da dobimo pri 590 nm v 5 cm plasti 85 % prepustnost svetlobe ali v 2 cm plasti 92 % prepustnost, pri čemer je fotometer naravnan na 100 % prepustnost na nenacepljenem osnovnem gojišču.
English: PLACE 9 ML OF THE INOCULATED CULTURE MEDIUM IN EACH TUBE).
Slovenian: V vsako epruveto prenesemo 9 ml nacepljenega gojišča.
English: THEN BLOCK FURTHER GROWTH BY RAPIDLY INJECTING 1 ML OF FORMALDEHYDE SOLUTION INTO EACH TUBE.
Slovenian: Nato v vsako epruveto hitro vbrizgamo 1 ml raztopine formaldehida in s tem zaustavimo nadaljnjo rast.
English: MEASURE THE TRANSMISSIONS WITH THE PHOTOMETER AT 590 NM, SETTING THE APPARATUS AT 100 % TRANSMISSION ON THE CLEAREST STANDARD SOLUTION (CORRESPONDING TO THE HIGHEST ANTIBIOTIC CONTENT).
Slovenian: Pri 590 nm izmerimo prepustnost svetlobe, pri čemer smo fotometer naravnali na 100-odstotno prepustnost svetlobe pri najbistrejši standardni raztopini (kar ustreza največji koncentraciji antibiotika).
English: SINCE THE DIFFERENT TUBES WILL SHOW SLIGHT DIFFERENCES OF TURBIDITY, AT LEAST 2 CM, AND PREFERABLY 5 CM, CELLS SHOULD BE USED.
Slovenian: Ker se bodo pri različnih epruvetah pokazale rahle razlike v motnosti, uporabimo vsaj 2 cm, še bolje pa 5 cm celice.
English: THIS METHOD MAKES IT POSSIBLE, EVEN IN THE PRESENCE OF TETRACYCLINES, TO DETERMINE THE OLEANDOMYCIN CONTENT OF FEEDINGSTUFFS, CONCENTRATES AND PREMIXES, WHERE MORE THAN 0.5 PPM ARE PRESENT.
Slovenian: S to metodo je mogoče celo v navzočnosti tetraciklinov določiti vsebnost oleandomicina v krmi, koncentratih in premiksih, kjer je vsebnost večja od 0,5 ppm.
English: AFTER CENTRIFUGING, THE EXTRACT IS DILUTED AND ITS ANTIBIOTIC ACTIVITY DETERMINED BY MEASURING THE DIFFUSION OF THE OLEANDOMYCIN ON AN AGAR MEDIUM SEEDED WITH B. CEREUS.
Slovenian: Po centrifugi ekstrakt razredčimo in določimo njegovo antibiotično aktivnost tako, da izmerimo difuzijo oleandomicina na agarskem gojišču, ki je nacepljeno z B. cereus.
English: CEREUS K 250 TR (RESISTANT TO TETRACYCLINES)
Slovenian: cereus K 250 TR (odporen proti tetraciklinom)
English: INOCULATE WITH B. CEREUS A TUBE OF SLOPED AGAR TAKEN FROM THE CULTURE MEDIUM TO WHICH 100 UG PER 5 ML OF OXYTETRACYCLINE HAS BEEN ADDED.
Slovenian: V epruvete s poševnim nanosom agarja z gojišča, ki smo mu dodali 100 g na 5 ml oksitetraciklina, nacepimo B. cerus.
English: COLLECT THE BACTERIA FROM A TUBE OF SLOPED AGAR USING APPROXIMATELY 3 ML OF PHYSIOLOGICAL SALINE.
Slovenian: S 3 ml fiziološke raztopine posnamemo bakterije iz epruvete s poševnim nanosom agarja.
English: WITH THIS SUSPENSION, SEED A ROUX FLASK CONTAINING 300 ML OF CULTURE MEDIUM WHICH HAS A 3 TO 4 % AGAR CONCENTRATION.
Slovenian: To suspenzijo zasejemo v Rouxovi steklenici, ki vsebuje 300 ml gojišča, s 3- do 4-odstotno koncentracijo agarja.
English: BY PRELIMINARY TESTS ON PLATES WITH THE BASIC MEDIUM FOR THE DETERMINATION, ESTABLISH THE QUANTITY OF INOCULUM WHICH, FOR THE DIFFERENT CONCENTRATIONS OF OLEANDOMYCIN USED, WILL GIVE THE LARGEST POSSIBLE INHIBITION ZONES THAT ARE STILL CLEAR.
Slovenian: Na podlagi poprejšnjih preskusov na ploščah z osnovnim gojiščem določimo količino nacepljenja, ki bo pri različnih koncentracijah oleandomicina povzročil nastanek največjih možnih in še vedno povsem razvidnih inhibicijskih con.
English: MEDIUM ADJUSTED TO PH 8.8.
Slovenian: Gojišče, vrednost pH naravnana na 8,8.
English: STANDARD SUBSTANCE:
Slovenian: Standardna snov:
English: DISSOLVE SOME OF THE STANDARD SUBSTANCE IN 5 ML OF METHANOL AND DILUTE WITH THE SOLUTION TO OBTAIN AN OLEANDOMYCIN CONCENTRATION OF 100 UG PER ML.
Slovenian: Nekaj standardne raztopine raztopimo v 5 ml metanola in razredčimo z raztopino, da dobimo koncentracijo oleandomicina 100 g na ml.
English: FROM THIS STOCK SOLUTION, PREPARE A STANDARD WORKING SOLUTION S8 CONTAINING 0.1 UG PER ML OF OLEANDOMYCIN BY DILUTING WITH THE SOLUTION.
Slovenian: To osnovno raztopino razredčimo z raztopino in tako pripravimo standardno delovno raztopino S8, ki vsebuje 0,1 g na ml oleandomicina.
English: THEN PREPARE BY SUCCESSIVE DILUTIONS (1 + 1), USING THE SOLUTION, THE FOLLOWING CONCENTRATIONS:
Slovenian: Nato z dodatnim razredčevanjem (1 + 1) z raztopino pripravimo naslednje koncentracije:
English: TAKE A TEST SAMPLE OF 2 TO 10 G, DEPENDING ON THE PRESUMED OLEANDOMYCIN CONTENT OF THE SAMPLE, ADD 100 ML OF THE SOLUTION AND SHAKE FOR 30 MINUTES ON A SHAKING PLATFORM.
Slovenian: Vzamemo od 2 do 10 g preskusnega vzorca, odvisno od pričakovane koncentracije oleandomicina v vzorcu, dodamo 100 ml raztopine in stresamo 30 minut na stresalni plošči.
English: CENTRIFUGE, TAKE AN ALIQUOT PART OF THE EXTRACT AND DILUTE WITH THE SOLUTION TO OBTAIN A PRESUMED OLEANDOMYCIN CONCENTRATION OF 0.1 UG PER ML (= U8).
Slovenian: Po centrifugi vzamemo alikvotni delež ekstrakta in ga razredčimo z raztopino, da dobimo pričakovano vsebnost oleandomicina 0,1 g na ml (= U8).
English: INOCULATE AT 60* C THE BASIC MEDIUM FOR THE DETERMINATION WITH THE SPORE SUSPENSION.
Slovenian: Osnovno gojišče za določanje nacepimo pri 50 do 60 °C s suspenzijo spor.
English: DIFFUSION ON AGAR IS CARRIED OUT IN TRAYS USING 4 CONCENTRATIONS OF THE STANDARD SOLUTION (S8, S4, S2, S1) AND FOUR CONCENTRATIONS OF THE EXTRACT (U8, U4, U2, U1).
Slovenian: Difuzija na agarju se izvaja na ploščah s štirimi koncentracijami standardne raztopine (S8, S4, S2, S1) in s štirimi koncentracijami ekstrakta (U8, U4, U2, U1).
English: PIPETTE INTO THE HOLES ACCURATELY MEASURED QUANTITIES OF BETWEEN 0.10 AND 0.15 ML OF ANTIBIOTIC SOLUTION, DEPENDING ON THE DIAMETER OF THE HOLES.
Slovenian: V vdolbine nakapamo natančno odmerjene količine raztopine antibiotika, od 0,10 do 0,15 ml, odvisno od premera vdolbin.
English: PURPOSE AND SCOPE THIS METHOD MAKES IT POSSIBLE TO DETERMINE THE TYLOSIN CONTENT OF FEEDINGSTUFFS, CONCENTRATES AND PREMIXES WHERE MORE THAN 2 PPM ARE PRESENT.
Slovenian: Namen in področje uporabe s to metodo je mogoče določiti vsebnost tilozina v krmi, koncentratih in premiksih pri vsebnostih nad 2 ppm.
English: THE SAMPLE IS TREATED WITH A PH 8 PHOSPHATE BUFFER SOLUTION, PREVIOUSLY HEATED TO 80* C, AND THEN EXTRACTED WITH METHANOL.
Slovenian: Vzorec obdelamo z raztopino fosfatnega puferja (pH 8), ki smo ga poprej segreli na 80 °C, in ga nato ekstrahiramo z metanolom.
English: AFTER CENTRIFUGING, THE EXTRACT IS DILUTED AND ITS ANTIBIOTIC ACTIVITY DETERMINED BY MEASURING THE DIFFUSION OF THE TYLOSIN ON AN AGAR MEDIUM SEEDED WITH SARCINA LUTEA.
Slovenian: Po centrifugiranju ekstrakt razredčimo in določimo njegovo antibiotično aktivnost tako, da izmerimo difuzijo tilozina na agarskem gojišču, ki je nacepljeno s Sarcina lutea.
English: INOCULATE WITH SARCINA LUTEA A TUBE OF SLOPED AGAR TAKEN FROM THE CULTURE MEDIUM, ADJUST TO PH 7.0.
Slovenian: V epruveti s poševnim nanosom agarja nacepimo s Sarcina lutea gojišče in vrednost pH naravnamo na 7,0.
English: COLLECT THE BACTERIA FROM A RECENTLY PREPARED TUBE OF SLOPED AGAR USING 2 TO 3 ML OF PHYSIOLOGICAL SALINE.
Slovenian: Z 2 do 3 ml fiziološke raztopine posnamemo bakterije iz epruvete s poševnim nanosom agarja , ki smo jo pripravili pred kratkim.
English: WITH THIS SUSPENSION SEED A ROUX FLASK CONTAINING 250 ML OF THE CULTURE MEDIUM, ADJUSTED TO PH 7.0.
Slovenian: S to suspenzijo zasejemo v Rouxovi steklenici 250 ml gojišča, katerega vrednost pH je bila naravnana na 7,0.
English: INCUBATE FOR 24 HOURS AT 35* C, THEN COLLECT THE BACTERIA IN 25 ML OF PHYSIOLOGICAL SALINE.
Slovenian: Po inkubaciji, ki traja 24 ur pri 35 °C, bakterijo posnamemo v 25 ml fiziološke raztopine in homogeniziramo.
English: HOMOGENIZE, AND DILUTE THIS SUSPENSION TO OBTAIN APPROXIMATELY 75 % LIGHT TRANSMISSION AT 650 NM.
Slovenian: To suspenzijo nato razredčimo, da dobimo pri 650 nm približno 75-odstotno prepustnost svetlobe.
English: BY PRELIMINARY TESTS ON PLATES WITH THE BASIC MEDIUM FOR THE DETERMINATION, ESTABLISH THE QUANTITY OF INOCULUM WHICH, FOR THE DIFFERENT CONCENTRATIONS OF TYLOSIN USED, WILL GIVE THE LARGEST POSSIBLE INHIBITION ZONES THAT ARE STILL CLEAR.
Slovenian: S poprejšnjimi preskusi na ploščah z gojiščem ugotovimo količino cepiva, ki bo pri različnih koncentracijah uporabljenega tilozina dala največje možne in še vedno povsem jasne inhibicijske cone.
English: ADJUST IMMEDIATELY BEFORE USE TO PH 7.0 FOR MAINTENANCE OF THE PARENT STRAIN AND PREPARATION OF THE BACTERIA SUSPENSION, AND TO PH 8.0 FOR THE DETERMINATION.
Slovenian: Za vzdrževanje osnovnega seva in pripravo suspenzije bakterij tik pred uporabo naravnamo pH na 7,0, za določanje pa pH na 8,0.
English: MIXTURE OF PHOSPHATE BUFFER SOLUTION/PURE METHANOL:
Slovenian: Mešanica raztopine fosfatnega puferja/čistega metanola:
English: 60/40 BY VOLUME.
Slovenian: 60/40 po prostornini.
English: STANDARD SOLUTIONS DRY THE STANDARD SUBSTANCE FOR 3 HOURS AT 60* C IN A VACUUM OVEN (5 MM OF MERCURY).
Slovenian: Standardne raztopine Standardno snov sušimo 3 ure pri 60 °C v vakuumskem sušilniku (5 mm živega srebra).
English: WEIGH 10 TO 50 MG INTO A GRADUATED FLASK, DISSOLVE IN 5 ML OF METHANOL AND DILUTE THE SOLUTION WITH THE PHOSPHATE BUFFER SOLUTION, PH 7, TO OBTAIN A TYLOSIN-BASE CONCENTRATION OF 1 000 UG PER ML.
Slovenian: Natehtamo od 10 do 50 mg v merilno bučko, raztopimo v 5 ml metanola in to raztopino razredčimo z raztopino fosfatnega puferja, pH 7, da dobimo koncentracijo tilozina 1000 g na ml.
English: PREPARE A STANDARD WORKING SOLUTION S8 CONTAINING 2 UG PER ML OF TYLOSIN BASE FROM THIS STOCK SOLUTION BY DILUTING WITH THE MIXTURE.
Slovenian: Osnovno raztopino razredčimo z mešanico, da dobimo standardno delovno raztopino S8, ki vsebuje 2 g na ml tilozina.
English: THEN PREPARE BY SUCCESSIVE DILUTIONS (1 + 1), USING THE MIXTURE, THE FOLLOWING CONCENTRATIONS:
Slovenian: Nato z dodatnim razredčevanjem (1 + 1) z mešanico pripravimo naslednje koncentracije:
English: FOR CONCENTRATES, TAKE A 10 G TEST SAMPLE; FOR PREMIXES AND FEEDINGSTUFFS, A 20 G TEST SAMPLE.
Slovenian: Za koncentrate vzamemo 10 g preskusnega vzorca, za premikse in krmo pa 20 gramov.
English: ADD 60 ML OF PHOSPHATE BUFFER SOLUTION, PH 8, PREVIOUSLY HEATED TO 80* C, AND HOMOGENIZE FOR 2 MINUTES (DOMESTIC MIXER, ULTRA-TURRAX ETC.).
Slovenian: Dodamo 60 ml raztopine fosfatnega puferja, pH 8, ki smo ga poprej segreli na 80 °C, in 2 minuti mešamo (gospodinjski mešalnik, Ultra-turrax itn.).
English: LEAVE TO STAND FOR 10 MINUTES, ADD 40 ML OF METHANOL AND HOMOGENIZE FOR 5 MINUTES.
Slovenian: Pustimo mirovati 10 minut, dodamo 40 ml metanola in mešamo še 5 minut.
English: CENTRIFUGE THE EXTRACT AND DILUTE AN ALIQUOT PART WITH THE MIXTURE TO OBTAIN A PRESUMED TYLOSIN CONCENTRATION OF 2 UG PER ML (= U8).
Slovenian: Ekstrakt centrifugiramo in alikvot razredčimo z mešanico, da dobimo pričakovano vsebnost tilozina 2 g na ml (= U8).
English: THEN PREPARE THE CONCENTRATION U4, U2 AND U1 BY SUCCESSIVE DILUTIONS (1 + 1) USING THE MIXTURE.
Slovenian: Nato z zaporednim razredčevanjem (1 + 1) z mešanico pripravimo koncentracije U4, U2 in U1.
English: FOR CONTENTS OF LESS THAN 10 PPM, EVAPORATE THE EXTRACT UNTIL DRY IN A ROTARY EVAPORATOR AT 35* C AND DISSOLVE THE RESIDUE IN 40 % METHANOL.
Slovenian: Pri vsebnostih pod 10 ppm uparimo ekstrakt v rotacijskem uparjalniku pri 35 °C do suhega, ostanek pa raztopimo v 40-odstotnem metanolu.
English: INOCULATE AT 48 TO 50* C THE BASIC MEDIUM FOR THE DETERMINATION, ADJUST TO PH 8.0, WITH THE BACTERIA SUSPENSION.
Slovenian: Osnovno gojišče za določanje nacepimo pri 48 do 50 °C s suspenzijo bakterij in naravnamo pH na 8,0.
English: THE TEST SHOULD PREFERABLY BE CARRIED OUT ON FLAT TRAYS CONSISTING OF GLASS PLATES FITTED WITH A PERFECTLY LEVEL ALUMINIUM OR PLASTIC RING, 200 MM IN DIAMETER AND 20 MM HIGH.
Slovenian: Najbolje, da se preskus izvaja na ploščah s plosko stekleno površino, ki imajo popolnoma raven aluminijev ali plastični obroč, premera 200 mm in višine 20 mm.