FrancophonIA/DGT-Translation_Memory huggingface dataset:
English: PIPETTE 5.0 ML OF HAEMOGLOBIN SOLUTION INTO A TEST TUBE, HEAT TO 25* C IN THE WATER BATH, ADD 1.0 ML OF THE PEPSIN SOLUTION OBTAINED IN 5.1 AND MIX WITH A GLASS ROD THICKENED AT ONE END, WITH ABOUT 10 BACK-AND-FORTH MOVEMENTS.
Slovenian: V epruveto odpipetiramo 5,0 ml raztopine hemoglobina, na vodni kopeli jo segrejemo na 25 °C, dodamo 1,0 ml raztopine pepsina, dobljene po navodilih iz 5.1, in s stekleno paličico, odebeljeno na enem koncu, mešamo tekočino tako, da desetkrat potegnemo skoznjo naprej in nazaj.
English: LEAVE THE TEST TUBE IN THE WATER BATH AT 25* C FOR EXACTLY 10 MINUTES, TIMED FROM THE ADDITION OF THE PEPSIN SOLUTION
Slovenian: Epruveto pustimo na vodni kopeli pri 25 °C točno 10 minut po tem, ko smo dodali raztopino pepsina
English: THEN ADD 10.0 ML OF TRICHLOROACETIC ACID SOLUTION PREVIOUSLY HEATED TO 25* C, HOMOGENIZE AND FILTER THROUGH A DRY FILTER.
Slovenian: Nato dodamo 10,0 ml raztopine triklorocetne kisline, ki smo jo poprej segreli na 25 °C, premešamo in filtriramo skozi suh filter.
English: PIPETTE 5.0 ML OF THE FILTRATE INTO A 50 ML ERLENMEYER FLASK, ADD 10.0 ML OF SODIUM HYDROXIDE SOLUTION AND, SHAKING CONSTANTLY, 3.0 ML OF DILUTE FOLIN-CIOCALTEU REAGENT.
Slovenian: 5,0 ml filtrata odpipetiramo v 50-mililitrsko erlenmajerico, dodamo 10,0 ml raztopine natrijevega hidroksida in ob nenehnem stresanju še 3,0 ml razredčenega reagenta po Folin-Ciocalteuju.
English: AFTER 5 TO 10 MINUTES, DETERMINE THE OPTICAL DENSITY OF THE SOLUTION WITH THE SPECTROPHOTOMETER AT 750 NM IN CELLS 1 CM THICK AGAINST WATER.
Slovenian: Po 5 do 10 minutah določimo optično gostoto raztopine s spektrofotometrom pri 750 nm v 1 cm kiveti, proti vodi.
English: FOR EACH DETERMINATION, CARRY OUT A BLANK TEST AS FOLLOWS:
Slovenian: Za vsako določitev izvedemo slepi preskus:
English: PIPETTE 5.0 ML OF HAEMOGLOBIN SOLUTION INTO A TEST TUBE, HEAT TO 25* C IN THE WATER BATH, ADD 10.0 ML OF TRICHLOROACETIC ACID SOLUTION PREVIOUSLY HEATED TO 25* C, HOMOGENIZE, THEN ADD 1.0 ML OF THE PEPSIN SOLUTION OBTAINED IN 5.1.
Slovenian: V epruveto odpipetiramo 5,0 ml raztopine hemoglobina, na vodni kopelijo segrejemo na 25 °C, dodamo 10,0 ml raztopine triklorocetne kisline, ki smo jo poprej segreli na 25 °C, premešamo in nato dodamo 1,0 ml raztopine pepsina, dobljene po navodilih iz 5.1.
English: MIX WITH A GLASS ROD AND LEAVE THE TEST TUBE IN THE WATER BATH AT 25* C FOR EXACTLY 10 MINUTES.
Slovenian: Premešamo s stekleno paličico in pustimo epruveto na vodni kopeli pri 25°C točno 10 minut.
English: PLACE 1.0, 2.0, 3.0, 4.0 AND 5.0 ML ALIQUOTS OF STANDARD TYROSINE SOLUTION, CORRESPONDING TO 0.2, 0.4, 0.6, 0.8 AND 1.0 UMOLES OF TYROSINE RESPECTIVELY IN 50 ML ERLENMEYER FLASKS.
Slovenian: V 50-mililitrske erlenmajerice odmerimo 1,0, 2,0, 3,0, 4,0 in 5,0 ml standardne raztopine tirozina, kar ustreza 0,2, 0,4, 0,6, 0,8 in 1,0 -molom tirozina.
English: MAKE UP THE VOLUMES TO 5.0 ML WITH HYDROCHLORIC ACID.
Slovenian: Volumne dopolnimo do 5,0 ml s klorovodikovo kislino.
English: ADD 10.0 ML OF SODIUM HYDROXIDE SOLUTION AND, SHAKING CONSTANTLY, 3.0 ML OF DILUTE FOLIN-CIOCALTEU REAGENT.
Slovenian: Dodamo 10,0 ml raztopine natrijevega hidroksida in ob nenehnem stresanju še 3,0 ml razredčenega reagenta po Folin-Ciocalteuju.
English: THE QUANTITY OF PEPSIN TO BE DISSOLVED MUST BE SUCH THAT, ON FINAL PHOTOMETRIC MEASUREMENT, AN OPTICAL DENSITY OF 0.35 MORE OR LESS 0.035 IS OBTAINED.
Slovenian: Pepsina moramo raztopiti toliko, da dobimo pri končnih fotometričnih meritvah optično gostoto 0,35 0,035.
English: TWO UNITS PER MG OBTAINED BY THIS METHOD CORRESPOND TO:
Slovenian: Dve enoti na mg, dobljeni s to metodo, ustrezata:
English: ANSON MILLIUNITS/MG (UMOLES OF TYROSINE/MG MIN AT 35.5* C) OR
Slovenian: 3,64 Anson milienot/mg (-molov tirozina/mg minuto pri 35,5 °C) ali
English: 36 400 COMMERCIAL UNITS/G (UMOLES OF TYROSINE/G IN 10 MIN AT 35.5* C).
Slovenian: 36 400 komercialnih enot/g (-molov tirozina/g v 10 minutah pri 35,5 °C).
English: THIS METHOD MAKES IT POSSIBLE TO DETERMINE THE LEVELS OF FREE GOSSYPOL, TOTAL GOSSYPOL AND CHEMICALLY RELATED SUBSTANCES IN COTTONSEED, COTTONSEED MEAL AND COTTONSEED CAKE AND IN COMPOUND FEEDINGSTUFFS CONTAINING THESE SUBSTANCES WHERE MORE THAN 20 PPM ARE PRESENT.
Slovenian: S to metodo je v krmnih mešanicah mogoče določiti vsebnost prostega in celotnega gosipola ter kemijsko sorodnih snovi v semenu, moki in tropinah bombaževca, kjer je njihova vsebnost nad 20 ppm.
English: THE GOSSYPOL IS EXTRACTED IN THE PRESENCE OF 3-AMINOPROPAN-1-OL, EITHER WITH A MIXTURE OF PROPAN-2-OL AND HEXANE, FOR THE DETERMINATION OF FREE GOSSYPOL, OR WITH DIMETHYLFORMAMIDE, FOR THE DETERMINATION OF TOTAL GOSSYPOL.
Slovenian: Gosipol ekstrahiramo v navzočnosti 3-aminopropan-1-ola z mešanico izopropanola in heksana pri določanju prostega gosipola ali z dimetilformamidom pri določanju celotnega gosipola.
English: THE GOSSYPOL IS CONVERTED BY ANILINE INTO GOSSYPOL-DIANILINE, THE OPTICAL DENSITY OF WHICH IS MEASURED AT 440 NM.
Slovenian: Z anilinom gosipol pretvorimo v gosipol-dianilin, katerega optično gostoto izmerimo pri 440 nm.
English: PROPAN-2-OL-HEXANE MIXTURE:
Slovenian: Mešanica izopropanola in heksana:
English: SOLVENT A:
Slovenian: Topilo A:
English: PLACE IN A 1 LITRE GRADUATED FLASK APPROXIMATELY 500 ML OF PROPAN-2-OL-HEXANE MIXTURE, 2 ML OF 3-AMINOPROPAN-1-OL, 8 ML OF GLACIAL ACETIC ACID AND 50 ML OF WATER.
Slovenian: V litrsko merilno bučko vlijemo približno 500 ml mešanice izopropanola in heksana, 2 ml 3-amino-1-propanola, 8 ml ledoceta in 50 ml vode.
English: MAKE UP TO VOLUME WITH THE PROPAN-2-OL-HEXANE MIXTURE.
Slovenian: Z mešanico izopropanola in heksana dopolnimo do oznake.
English: SOLVENT B:
Slovenian: Topilo B:
English: COOL TO ROOM TEMPERATURE AND MAKE UP TO VOLUME WITH N, N-DIMETHYLFORMAMIDE.
Slovenian: Ohladimo na sobno temperaturo in dopolnimo z N,N-dimetilformamidom do oznake.
English: ANILINE A. R.:
Slovenian: Anilin p.a.:
English: IF THE OPTICAL DENSITY IN THE BLANK TEST EXCEEDS 0.022, DISTIL THE ANILINE OVER ZINC DUST, DISCARDING THE FIRST AND LAST 10 % FRACTIONS OF THE DISTILLATE.
Slovenian: Če optična gostota v slepem preskusu presega 0,022, destiliramo anilin prek cinka v prahu, pri čemer zavržemo prvih in zadnjih 10 % destilata.
English: REFRIGERATED AND STORED IN A BROWN, STOPPERED GLASS FLASK, THIS REAGENT WILL KEEP FOR SEVERAL MONTHS.
Slovenian: Če reagent hranimo v rjavi, zaprti stekleni posodi v hladilniku, je obstojen več mesecev.
English: STANDARD GOSSYPOL SOLUTION A:
Slovenian: Standardna raztopina gosipola A:
English: PLACE 27.9 MG OF GOSSYPOL ACETATE IN A 250 ML GRADUATED FLASK. DISSOLVE AND MAKE UP TO VOLUME WITH SOLVENT A.
Slovenian: V 250-mililitrski merilni bučki raztopimo 27,9 mg gosipolacetata s topilom A in z njim dopolnimo bučko do oznake.
English: STANDARD GOSSYPOL SOLUTION B:
Slovenian: Standardna raztopina gosipola B:
English: PLACE 27.9 MG OF GOSSYPOL ACETATE IN A 50 ML GRADUATED FLASK. DISSOLVE AND MAKE UP TO VOLUME WITH SOLVENT B.
Slovenian: V 50-mililitrski merilni bučki raztopimo 27,9 mg gosipolacetata s topilom B in z njim dopolnimo bučko do oznake.
English: MIXER (TUMBLER):
Slovenian: Mešalec:
English: IT IS PREFERABLE TO WORK WITH A SMALL TEST SAMPLE AND A RELATIVELY LARGE ALIQUOT PART OF THE FILTRATE, SO AS TO OBTAIN SUFFICIENT GOSSYPOL FOR PRECISE PHOTOMETRIC MEASUREMENT TO BE POSSIBLE.
Slovenian: Primerneje je uporabiti majhen preskusni vzorec in relativno velik alikvot filtrata, da dobimo dovolj gosipola, s katerim omogočimo natančne fotometrične meritve.
English: FOR THE DETERMINATION OF FREE GOSSYPOL IN COTTONSEED, COTTONSEED MEAL AND COTTONSEED CAKE, THE TEST SAMPLE SHOULD NOT EXCEED 1 G; FOR COMPOUND FEEDINGSTUFFS, IT MAY BE AS MUCH AS 5 G.
Slovenian: Za določanje prostega gosipola v semenu, moki in tropinah bombaževca preskusni vzorec ne sme presegati 1 g, pri krmnih mešanicah pa ga je lahko tudi 5 g.
English: FOR THE DETERMINATION OF TOTAL GOSSYPOL, THE TEST SAMPLE SHOULD BE BETWEEN 0.5 AND 5 G, THAT A 2 ML ALIQUOT PART OF FILTRATE WILL CONTAIN 40 TO 200 UG OF GOSSYPOL.
Slovenian: Za določanje celotnega gosipola naj bi imeli od 0,5 do 5 g preskusnega vzorca, da bo 2-mililitrski alikvot filtrata vseboval od 40 do 200 g gosipola.
English: PLACE THE TEST SAMPLE IN A GROUND-NECKED 250 ML FLASK, THE BOTTOM OF THE FLASK HAVING BEEN COVERED WITH CRUSHED GLASS.
Slovenian: Preskusni vzorec prenesemo v 250-mililitrsko bučko z brusom, katere dno je prekrito z drobci stekla.
English: USING A PIPETTE, ADD 50 ML OF SOLVENT A, STOPPER THE FLASK AND MIX FOR ONE HOUR IN THE MIXER.
Slovenian: S pipeto dodamo 50 ml topila A, bučko zamašimo in z mešalnikom mešamo eno uro.
English: IF NECESSATY, MAKE UP THE VOLUME TO 10 ML WITH SOLVENT A.
Slovenian: Po potrebi dopolnimo do 10 ml s topilom A.
English: THEN MAKE THE CONTENTS OF FLASK (A) UP TO VOLUME WITH THE PROPAN-2-OL-HEXANE MIXTURE.
Slovenian: Bučko (A) dopolnimo z mešanice izopropanola in heksana do oznake.
English: PIPETTE 10 ML OF SOLVENT A INTO EQCH OF TWO OTHER 25 ML GRADUATED FLASKS (C AND D).
Slovenian: V dve drugi 25-mililitrski merilni bučki (C in D) odpipetiramo 10 ml topila A.
English: MAKE THE CONTENTS OF FLASK (C) UP TO VOLUME WITH THE PROPAN-2-OL-HEXANE MIXTURE.
Slovenian: Bučko (C) dopolnimo z mešanico izopropanola in heksana do oznake.
English: ADD 2 ML OF ANILINE TO EACH OF FLASKS (D) AND (B).
Slovenian: V vsako od bučk (D) in (B) dodamo po 2 ml anilina.
English: COOL TO ROOM TEMPERATURE, MAKE UP TO VOLUME WITH THE PROPAN-2-OL-HEXANE MIXTURE, HOMOGENIZE AND LEAVE TO STAND FOR ONE HOUR.
Slovenian: Ohladimo ju na sobno temperaturo, dopolnimo z mešanico izopropanola in heksana do oznake, premešamo in pustimo stati eno uro.
English: DETERMINE THE OPTICAL DENSITY OF THE BLANK TEST SOLUTION (D) BY COMPARISON WITH THE REFERENCE SOLUTION (C), AND THE OPTICAL DENSITY OF THE SAMPLE SOLUTION (B) BY COMPARISON WITH THE REFERENCE SOLUTION (A), IN THE SPECTROPHOTOMETER AT 440 NM USING 1 CM GLASS CELLS.
Slovenian: V spektrofotometru pri 440 nm in 1 cm kiveti določimo optično gostoto raztopine iz slepega preskusa (D) tako, da jo primerjamo s primerjalno raztopino (C), optično gostoto raztopine vzorca (B) pa primerjamo s primerjalno raztopino (A).
English: PLACE A TEST SAMPLE CONTAINING 1 TO 5 MG OF GOSSYPOL IN A 50 ML GRADUATED FLASK AND ADD 10 ML OF SOLVENT B.
Slovenian: V 50-mililitrsko merilno bučko prenesemo preskusni vzorec, ki vsebuje od 1 do 5 mg gosipola, in dodamo 10 ml topila B.
English: AT THE SAME TIME, PREPARE A BLANK TEST, PLACING 10 ML OF SOLVENT B IN ANOTHER 50 ML GRADUATED FLASK.
Slovenian: Hkrati pripravimo slepi preskus, in to tako, da v drugo 50-mililitrsko merilno bučko vlijemo 10 ml topila B.
English: COOL TO ROOM TEMPERATURE AND MAKE THE CONTENTS OF EACH FLASK UP TO VOLUME WITH THE PROPAN-2-OL-HEXANE MIXTURE.
Slovenian: Nato ju ohladimo na sobno temperaturo in dopolnimo z mešanico izopropanola in heksana do oznake.
English: HOMOGENIZE AND LEAVE TO SETTLE FOR 10 TO 15 MINUTES, THEN FILTER AND COLLECT THE FILTRATES IN GROUND-NECKED FLASKS.
Slovenian: Premešamo in pustimo od 10 do 15 minut, da se usede, nato filtriramo v bučko z brusom.
English: PIPETTE 2 ML OF THE SAMPLE FILTRATE INTO EACH OF TWO 25 ML GRADUATED FLASKS, AND 2 ML OF THE BLANK TEST FILTRATE INTO EACH OF TWO OTHER 25 ML FLASKS.
Slovenian: V dve 25-mililitrski merilni bučki odpipetiramo po 2 ml filtrata vzorca, v dve drugi 25-mililitrski bučki pa po 2 ml filtrata iz slepega preskusa.
English: MAKE THE CONTENTS OF ONE FLASK FROM EACH SERIES UP TO 25 ML WITH THE PROPAN-2-OL-HEXANE MIXTURE.
Slovenian: Po eno od bučk iz vsake serije dopolnimo z mešanico izopropanola in heksana do oznake.
English: ADD 2 ML OF ANILINE TO EACH OF THE OTHER TWO FLASKS.
Slovenian: V vsako od drugih dveh bučk dodamo po 2 ml anilina.
English: COOL TO ROOM TEMPERATURE, MAKE UP TO 25 ML WITH THE PROPAN-2-OL-HEXANE MIXTURE, HOMOGENIZE AND LEAVE TO STAND FOR ONE HOUR.
Slovenian: Ohladimo ju na sobno temperaturo, dopolnimo z mešanico izopropanola in heksana do oznake, premešamo in pustimo stati eno uro.
English: THIS METHOD MAKES IT POSSIBLE TO DETECT AND IDENTIFY ANTIBIOTICS OF THE TETRACYCLINE GROUP IN FEEDINGSTUFFS CONTAINING AT LEAST 0.1 PPM OF ANTIBIOTICS, IN CONCENTRATES AND IN PREMIXES.
Slovenian: S to metodo je mogoče odkrivati in identificirati antibiotike iz skupine tertraciklinov v krmi, ki vsebujejo vsaj 0,1 ppm antibiotikov, v koncentratih in premiksih.
English: THE ANTIBIOTICS ARE DETECTED AND IDENTIFIED BY COMPARING THEIR RF VALUES WITH THOSE OF THE STANDARD SUBSTANCES, EITHER BY FLUORESCENCE IN UV LIGHT (HIGH ANTIBIOTIC CONTENTS) OR BY BIOAUTOGRAPHY ON AN AGAR MEDIUM INOCULATED WITH B. CEREUS.
Slovenian: Antibiotike odkrivamo in identificiramo na podlagi primerjave njihovih vrednosti z vrednostmi standardnih snovi, bodisi s fluorescenčno mikroskopijo v UV svetlobi (visoka vsebnost antibiotikov) bodisi z bioavtografijo na agarskem gojišču, ki je nacepljeno z B. cereus.
English: CITRIC ACID MONOHYDRATE A. R. 10.256 G DISODIUM HYDROGEN PHOSPHATE NA2HPO4.2H2O A. R. 7.45 G
Slovenian: Monohidrat citronove kisline p.a. 10,256 g Dinatrijev hidrogenfosfat Na2HPO4 × 2H2O p.a. 7,45 g
English: PHOSPHATE BUFFER SOLUTION, PH 5.5 POTASSIUM DIHYDROGEN PHOSPHATE KH2PO4 A. R. 130.86 G
Slovenian: Raztopina fosfatnega puferja, pH 5,5 Kalijev dihidrogenfosfat KH2PO4 p.a. 130,86 g
English: ELUENT I:
Slovenian: Topilo I:
English: MIXTURE OF PURE NITROMETHANE/PURE CHLOROFORM/1, 3-DICHLOROPROPAN-2-OL:
Slovenian: Mešanica čistega nitrometana/čistega kloroforma/1,3-diklor-izopropanola:
English: 20/10/1.5 BY VOLUME PREPARE IMMEDIATELY BEFORE USE.
Slovenian: 20/10/1,5 po prostornini, pripravimo tik pred uporabo.
English: ELUENT II:
Slovenian: Topilo II:
English: MIXTURE OF PURE NITROMETHANE/PURE CHLOROFORM/2-PICOLINE:
Slovenian: Mešanica čistega nitrometana/čistega kloroforma/2-piridina:
English: MIXTURE OF PURE METHANOL/HYDROCHLORIC ACID (D: 1.19):
Slovenian: Mešanica čistega metanola/klorovodikove kisline (d: 1,19):
English: AMMONIA, D: 0.91.
Slovenian: Amonijak, d: 0,91.
English: STANDARD SUBSTANCES:
Slovenian: Standardne snovi:
English: CHLORTETRACYCLINE, OXYTETRACYCLINE, TETRACYCLINE, THE ACTIVITY OF WHICH IS EXPRESSED IN TERMS OF HYDROCHLORIDE.
Slovenian: Klortetraciklin, oksitetraciklin, tetraciklin, katerih aktivnost izrazimo na podlagi klorovodika.
English: MICRO-ORGANISM:
Slovenian: Mikroorganizem:
English: MAINTENANCE OF THE PARENT STRAIN, PREPARATION OF THE SPORE SUSPENSION AND INOCULATION OF THE CULTURE MEDIUM:
Slovenian: Ohranitev prvotne kulture, priprava suspenzije spor in nacepljenje gojišča:
English: FOLLOW THE DIRECTIONS GIVEN IN 3.1 AND 3.2 OF THE METHOD FOR THE DETERMINATION OF CHLORTETRACYCLINE, OXYTETRACYCLINE AND TETRACYCLINE CONTENTS BY DIFFUSION ON AGAR WHICH IS DESCRIBED IN PART 2 OF THIS ANNEX.
Slovenian: Sledimo navodilom iz 3.1 in 3.2 dela II te priloge o metodi za določanje vsebnosti klortetraciklina, oksitetraciklina in tetraciklina z difuzijo na agarskem gojišču.
English: % (W/V) 2, 3, 5-TRIPHENYLTETRAZOLIUM CHLORIDE SOLUTION AND 5 % (W/V) GLUCOSE SOLUTION.
Slovenian: 0,1 % (m/v) raztopine 2, 3, 5-trifeniltetrazoliumklorida in 5 % (m/v) raztopine glukoze.
English: APPARATUS FOR ASCENDING PAPER CHROMATOGRAPHY (HEIGHT OF PAPER: 25 CM).
Slovenian: Naprava za papirno kromatografijo (višina papirja: 25 cm).
English: USING HYDROCHLORIC ACID, PREPARE FROM THE STANDARD SUBSTANCES SOLUTIONS WITH CONCENTRATIONS CORRESPONDING TO 500 UG PER ML OF CHLORTETRACYCLINE-HCL, OF OXYTETRACYCLINE-HCL, AND OF TETRACYCLINE-HCL.
Slovenian: S klorovodikovo kislino pripravimo iz standardnih snovi raztopine s koncentracijami, ki ustrezajo 500 g na ml klortetraciklina-HCl, oksitetraciklina-HCl in tetraciklina-HCl.
English: DILUTE THE SOLUTIONS WITH THE PHOSPHATE BUFFER SOLUTION TO OBTAIN SOLUTIONS WITH CONCENTRATIONS CORRESPONDING TO 100 UG PER ML OF CHLORTETRACYCLINE-HCL, OF OXYTETRACYCLINE-HCL AND OF TETRACYCLINE-HCL.
Slovenian: Z raztopino fosfatnega puferja razredčimo raztopine, da dobimo raztopine s koncentracijami, ki ustrezajo 100 g na ml klortetraciklina-HCl, oksitetraciklina-HCl in tetraciklina-HCl.
English: DILUTE THE SOLUTIONS WITH THE PHOSPHATE BUFFER SOLUTION TO OBTAIN SOLUTIONS WITH CONCENTRATIONS CORRESPONDING TO 5 UG PER ML OF CHLORTETRACYCLINE-HCL, OF OXYTETRACYCLINE-HCL AND OF TETRACYCLINE-HCL.
Slovenian: Z raztopino fosfatnega puferja razredčimo raztopine, da dobimo raztopine s koncentracijami, ki ustrezajo 5 g na ml klortetraciklina-HCl, oksitetraciklina-HCl in tetraciklina-HCl.
English: WHEN THE PRESUMED ANTIBIOTIC CONTENT IS LESS THAN 10 PPM, EITHER THE HOMOGENIZED SAMPLE OR THE FINEST FRACTION SEPARATED BY SIEVING MAY BE USED, SINCE THE ANTIBIOTICS ARE TO BE FOUND MAINLY IN THIS FRACTION.
Slovenian: Kadar znaša pričakovana vsebnost antibiotika manj kakor 10 ppm, lahko uporabimo bodisi homogenizirani vzorec bodisi najčistejšo frakcijo, ki smo jo dobili s sejanjem, saj bomo antibiotike našli predvsem v tej frakciji.
English: SUSPEND THE SAMPLE IN THE MIXTURE AND CENTRIFUGE.
Slovenian: Vzorec suspendiramo v mešanici in centrifugiramo.
English: COLLECT THE SUPERNATANT LIQUID AND USE IT DIRECTLY OR DILUTE, IF NECESSARY, WITH THE MIXTURE TO OBTAIN ANTIBIOTIC CONCENTRATIONS OF APPROXIMATELY 100 UG PER ML AND 5 UG PER ML.
Slovenian: Bistro tekočino nad usedlino neposredno uporabimo ali po potrebi razredčimo z mešanico, da dobimo naslednje koncentracije antibiotika: približno 100 g na ml in 5 g na ml.
English: IMMERSE THE PAPER IN THE BUFFER SOLUTION, PH 3.5.
Slovenian: Papir potopimo v pufrsko raztopino, pH 3,5.
English: THEN PLACE VOLUMES OF 0.01 ML OF THE REFERENCE SOLUTIONS AND OF THE EXTRACT ON THE PAPER.
Slovenian: Nato nakapamo na papir 0,01 ml primerjalnih raztopin in prav toliko ekstraktov.
English: TO GIVE A GOOD SEPARATION, THE PAPER MUST HAVE THE CORRECT MOISTURE CONTENT;
Slovenian: Za dobro separacijo mora imeti papir pravo vsebnost vlage;
English: IF NECESSARY, LEAVE TO DRY A LITTLE.
Slovenian: po potrebi pustimo, da se rahlo osuši.
English: USE ELUENT I FOR DETECTION BY BIOAUTOGRAPHY AND ELUENT II FOR DETECTION BY UV LIGHT.
Slovenian: Za odkrivanje z bioavtobiografijo uporabimo topilo I in topilo II za odkrivanje z ultravijolično svetlobo.
English: WHEN THE SOLVENT FRONT HAS CLIMBED 15 TO 20 CM (APPROX. 1 HOUR 30 MINUTES), STOP CHROMATOGRAPHY AND DRY THE PAPER.
Slovenian: Ko se povzpne za 15 do 20 cm (približno v 1 uri in 30 minut), kromatografijo prekinemo in papir osušimo.
English: IF THE ANTIBIOTIC LEVEL IS GREATER THAN 1 UG PER CM2, AFTER THE CHROMATOGRAM HAS BEEN TREATED WITH AMMONIA VAPOURS GOLDEN YELLOW FLUORESCENT SPOTS WILL BE SEEN ON IRRADIATION UNDER THE UV LAMP.
Slovenian: Če je raven antibiotika višja od 1 g na cm2, bodo po obdelavi kromatograma z amonijakovo paro pri osvetlitvi z ultravijolično svetilko postali vidni zlato rumeni fluorescenčni madeži.
English: POUR THE CULTURE MEDIUM, PREVIOUSLY INOCULATED WITH B, CEREUS, INTO GLASS PLATES AND PLACE THE PAPER ON THE CULTURE MEDIUM.
Slovenian: Gojišče, ki smo ga poprej cepili z B. cerus, nanesemo na steklene plošče in nanj položimo papir.
English: AFTER 5 MINUTES' CONTACT, DETACH THE PAPER AND PLACE IT ON ANOTHER SPOT IN THE CULTURE MEDIUM, WHERE IT WILL REMAIN DURING THE INCUBATION PERIOD.
Slovenian: Po petih minutah papir odstranimo in ga položimo na drugo mesto na gojišču, kjer naj ostane ves čas inkubacijske dobe.
English: IF AN ANTIBIOTIC OF THE TETRACYCLINE GROUP IS PRESENT, LIGHT INHIBITION ZONES WILL APPEAR IN THE CLOUDY CULTURE MEDIUM.
Slovenian: Če gojišče vsebuje antibiotik iz skupine tetraciklinov, se bodo na drugače motnem gojišču kulture pokazale jasne inhibicijske cone.
English: TO FIX THE CHROMATOGRAM, THE SOLUTION IS VAPORIZED ON THE PAPER, AFTER INCUBATION.
Slovenian: Za fiksiranje kromatograma razpršimo papir po inkubaciji z raztopino.
English: THESE VALUES MAY VARY SLIGHTLY ACCORDING TO THE QUALITY OF THE PAPER AND ITS MOISTURE CONTENT:
Slovenian: Te vrednosti se lahko rahlo spreminjajo, odvisno od kakovosti papirja in vsebnosti vlage:
English: THE ANTIBIOTIC ACTIVITY OF THE "EPI" COMPOUNDS IS LESS THAN THAT OF THE NORMAL COMPOUNDS.
Slovenian: Antibiotična aktivnost spojin "epi" je manjša od običajnih spojin.
English: THIS METHOD MAKES IT POSSIBLE TO DETERMINE THE LEVELS OF CHLORTETRACYCLINE (CTC), OXYTETRACYCLINE (OTC) AND TETRACYCLINE (TC) IN FEEDINGSTUFFS, CONCENTRATES AND PREMIXES WHERE MORE THAN 5 PPM ARE PRESENT.
Slovenian: S to metodo je mogoče določiti stopnje klortetraciklina (CTC), oksitetraciklina (OTC) in tetraciklina (TC) v krmi, koncentratih in premiksih, ki vsebujejo več kakor 5 ppm.
English: FOR CONTENTS OF 50 PPM OR LESS, THE SAMPLE IS EXTRACTED WITH DILUTE FORMAMIDE.
Slovenian: Pri vsebnostih, ki znašajo 50 ppm ali manj, vzorec ekstrahiramo z razredčenim formamidom.
English: FOR CONTENTS GREATER THAN 50 PPM, IT IS EXTRACTED WITH A MIXTURE OF ACETONE, WATER AND HYDROCHLORIC ACID, FOR THE DETERMINATION OF CTC, AND WITH A MIXTURE OF METHANOL AND HYDROCHLORIC ACID FOR THE DETERMINATION OF OTC AND TC.
Slovenian: Pri vsebnostih, ki presegajo 50 ppm, ekstrahiramo vzorec z mešanico acetona, vode in klorovodikove kisline, kadar določamo CTC, ter z mešanico metanola in klorovodikove kisline, kadar določamo OTC in TC.
English: CEREUS, ATCC NO 11.778
Slovenian: cereus, ATCC št. 11 778
English: INOCULATE WITH B. CEREUS A TUBE OF SLOPED AGAR TAKEN FROM CULTURE MEDIUM FREE FROM METHYLENE BLUE AND BORIC ACID.
Slovenian: V epruvete s poševnim nanosom agarja, ki ne vsebuje borove kisline in metilensko modre, nacepimo B. cereus.
English: COLLECT THE BACTERIA FROM A TUBE OF SLOPED AGAR USING 2 TO 3 ML OF PHYSIOLOGICAL SALINE.
Slovenian: Z 2 do 3 ml fiziološke raztopine poberemo bakterije iz epruvete z agarskim gojiščem.
English: WITH THIS SUSPENSION, SEED A ROUX FLASK CONTAINING 300 ML OF CULTURE MEDIUM, FREE FROM METHYLENE BLUE AND BORIC ACID, WITH 3 TO 4 % AGAR CONCENTRATION.
Slovenian: To suspenzijo zasejemo v Rouxovi steklenici, ki vsebuje 300 ml gojišča, brez metilensko modre in borove kisline, s 3- do 4-odstotno koncentracijo agarja.
English: INCUBATE FOR 3 TO 5 DAYS AT 28 TO 30* C,
Slovenian: Inkubacija traja od 3 do 5 dni pri 28 do 30 °C.
English: THEN COLLECT THE SPORES IN 15 ML OF ETHANOL AFTER CHECKING SPORULATION UNDER A MICROSCOPE, AND HOMOGENIZE.
Slovenian: Nato mikroskopsko preverimo razvoj spor, jih posnamemo s 15 ml etanola in vse skupaj homogeniziramo.
English: BY PRELIMINARY TESTS ON PLATES WITH THE BASIC MEDIUM FOR THE DETERMINATION, ESTABLISH THE QUANTITY OF INOCULUM WHICH, FOR THE DIFFERENT CONCENTRATIONS OF ANTIBIOTIC USED, WILL GIVE THE LARGEST POSSIBLE INHIBITION ZONES THAT ARE STILL CLEAR.
Slovenian: Na podlagi poprejšnjih preskusov na ploščah z osnovnim gojiščem določimo količino nacepljenja, ki bo pri različnih koncentracijah uporabljenega antibiotika povzročilo nastanek največjih možnih in še vedno povsem razvidnih inhibicijskih con.
English: "TWEEN 80" 1 ML PHOSPHATE BUFFER SOLUTION, PH 5.5 10 ML
Slovenian: "Tween 80" 1 ml Raztopina fosfatnega puferja, pH 5,5 10 ml
English: DISODIUM HYDROGEN PHOSPHATE
Slovenian: Dinatrijev hidrogenfosfat
English: PHOSPHATE BUFFER SOLUTION, PH 5.5, DILUTED TO 1/10.
Slovenian: Raztopina fosfatnega puferja, pH 5.5, razredčena na 1/10.
English: POTASSIUM DIHYDROGEN PHOSPHATE
Slovenian: Kalijev dihidrogenfosfat
English: 70 % (V/V) FORMAMIDE:
Slovenian: 70 % (v/v) formamid:
English: MIXTURE OF PURE ACETONE/WATER/HYDROCHLORIC ACID
Slovenian: Mešanica čistega acetona/vode/klorovodikove kisline
English: CTC, OTC, TC, THE ACTIVITY OF WHICH IS EXPRESSED IN TERMS OF HYDROCHLORIDE.
Slovenian: CTC, OTC, TC, katerih aktivnost izrazimo na podlagi klorovodika.
English: USING HYDROCHLORIC ACID, PREPARE FROM THE STANDARD SOLUTION A STOCK SOLUTION WITH A CONCENTRATION CORRESPONDING TO 500 UG PER ML OF CHLORTETRACYCLINE-HCL.
Slovenian: S klorovodikovo kislino pripravimo iz standardne raztopine osnovno raztopino s koncentracijo, ki ustreza 500 g na ml klortetraciklina-HCl.
English: FROM THIS STOCK SOLUTION, PREPARE A STANDARD WORKING SOLUTION S8 WITH A CONCENTRATION CORRESPONDING TO 0.2 UG PER ML OF CHLORTETRACYCLINE-HCL.
Slovenian: Iz te osnovne raztopine pripravimo standardno delovno raztopino S8 s koncentracijo, ki ustreza 0,2 g na ml klortetraciklina-HCl.