FrancophonIA/DGT-Translation_Memory huggingface dataset:
English: PURPOSE AND SCOPE THIS METHOD MAKES IT POSSIBLE TO DETERMINE THE VIRGINIAMYCIN CONTENT OF FEEDINGSTUFFS, CONCENTRATES AND PREMIXES WHERE MORE THAN 2 PPM ARE PRESENT.
Slovenian: Namen in področje uporabe s to metodo je mogoče določiti vsebnost virginiamicina v krmi, koncentracijah in premiksih, kjer je vsebnost večja od 2 ppm.
English: THE SAMPLE IS EXTRACTED WITH A " WEEN 80" METHANOL SOLUTION.
Slovenian: Vzorec ekstrahiramo z raztopino metanola "Tween 80".
English: AFTER CENTRIFUGING OR FILTERING, THE EXTRACT IS DILUTED AND ITS ANTIBIOTIC ACTIVITY DETERMINED BY MEASURING THE DIFFUSION OF THE VIRGINIAMYCIN ON AN AGAR MEDIUM SEEDED WITH SARCINA LUTEA.
Slovenian: Po centrifugi ali filtriranju ekstrakt razredčimo in določimo njegovo antibiotično aktivnost tako, da izmerimo difuzijo virginiamicina na agarskem gojišču, ki je nacepljeno s Sarcina lutea.
English: INOCULATE WITH S. LUTEA A TUBE OF SLOPED AGAR TAKEN FROM THE CULTURE MEDIUM.
Slovenian: V epruvete s poševnim nanosom agarja, ki izhaja iz gojišča, nacepimo s S. lutea.
English: WITH THIS SUSPENSION, SEED A ROUX FLASK CONTAINING 250 ML OF THE CULTURE MEDIUM.
Slovenian: To suspenzijo zasejemo v Rouxovi steklenici, ki vsebuje 250 ml gojišča.
English: BY PRELIMINARY TESTS ON PLATES WITH THE BASIC MEDIUM FOR THE DETERMINATION, ESTABLISH THE QUANTITY OF INOCULUM WHICH, FOR THE DIFFERENT CONCENTRATIONS OF VIRGINIAMYCIN USED, WILL GIVE THE LARGEST POSSIBLE INHIBITION ZONES THAT ARE STILL CLEAR.
Slovenian: Na podlagi poprejšnjih preskusov na ploščah z osnovnim gojiščem določimo količino nacepljenja, ki bo pri različnih koncentracijah uporabljenega virginiamicina povzročilo nastanek največjih možnih in še vedno povsem razvidnih inhibicijskih con.
English: 80/20 BY VOLUME.
Slovenian: 80/20 po prostornini.
English: % (W/V) "TWEEN 80" METHANOL SOLUTION.
Slovenian: 0,5 % (m/v) raztopine metanola "Tween 80".
English: STANDARD SOLUTIONS PREPARE A METHANOL SOLUTION OF THE STANDARD SUBSTANCE CONTAINING 800 UG PER ML OF VIRGINIAMYCIN.
Slovenian: Standardne raztopine Pripravimo metanolovo raztopino standardne snovi, ki vsebuje 800 g na ml virginiamicina.
English: FROM THIS STOCK SOLUTION PREPARE A STANDARD WORKING SOLUTION S8 CONTAINING 1 UG PER ML OF VIRGINIAMYCIN BY DILUTING WITH THE MIXTURE.
Slovenian: To osnovno raztopino razredčimo z raztopino in tako pripravimo standardno delovno raztopino S8, ki vsebuje 1 g na ml virginiamicina.
English: TAKE A TEST SAMPLE OF 10 TO 20 G, ADD 100 ML OF THE SOLUTION AND SHAKE FOR 30 MINUTES ON A SHAKING PLATFORM.
Slovenian: Vzamemo od 10 do 20 g preskusnega vzorca, dodamo 100 ml raztopine in stresamo 30 minut na stresalni plošči.
English: CENTRIFUGE OR FILTER, TAKE 20 ML OF THE CLEAR SOLUTION AND EVAPORATE UNTIL DRY IN A ROTARY EVAPORATOR.
Slovenian: Po centrifugi ali filtriranju vzamemo 20 ml bistre raztopine in pustimo, da izpareva v rotacijskem uparjalniku, dokler ni povsem suha.
English: DISSOLVE THE RESIDUE IN 20 ML OR MORE OF THE MIXTURE TO OBTAIN A PRESUMED VIRGINIAMYCIN CONCENTRATION OF 1 UG PER ML (= U8).
Slovenian: Ostanek raztopimo v 20 ml ali več mešanice, da dobimo pričakovano vsebnost virginiamicina 1 g na ml (= U8).
English: THEN PREPARE THE CONCENTRATIONS U4, U2 AND U1 BY SUCCESSIVE DILUTIONS (1 + 1) USING THE MIXTURE.
Slovenian: Nato z zaporednim razredčevanjem (1 + 1) z mešanico pripravimo koncentracije U4, U2 in U1.
English: TAKE A TEST SAMPLE OF 1 TO 10 G, ADD 100 ML OF SOLUTION AND SHAKE FOR 30 MINUTES ON A SHAKING PLATFORM.
Slovenian: Vzamemo od 1 do 10 g preskusnega vzorca, dodamo 100 ml raztopine in stresamo 30 minut na stresalni plošči.
English: CENTRIFUGE OR FILTER, THEN DILUTE WITH THE MIXTURE TO OBTAIN A PRESUMED VIRGINIAMYCIN CONCENTRATION OF 1 UG PER ML (= U8).
Slovenian: Po centrifugi ali filtriranju razredčimo z mešanico, da dobimo pričakovano vsebnost virginiamicina 1 g na ml (= U8).
English: THEN PREPARE THE CONCENTRATIONS U4, U2 AND U1 AS INDICATED IN 6.1.
Slovenian: Nato pripravimo koncentracije U4, U2 in U1, kakor je navedeno v 6.1.
English: INOCULATE THE BASIC MEDIUM FOR THE DETERMINATION AT 48 TO 50* C WITH THE SUSPENSION OF BACTERIA.
Slovenian: Osnovno gojišče za določanje nacepimo pri 50 do 60 °C s suspenzijo spor.
English: TO CHECK THE ACCURACY OF THE METHOD , CARRY OUT THE ANALYSIS (DIGESTION , DISTILLATION AND TITRATION) ON 1.5 TO 2.0 G OF ACETANILIDE A. R. (M. P. 114* C % N : 10.36) IN THE PRESENCE OF 1 G OF NITROGEN-FREE SUCROSE;
Estonian: Meetodi täpsuse kontrollimiseks tehakse analüüs 1,5-2,0 g analüütiliselt puhta atseetaniliidiga (st 114 °C % N: 10,36) 1 g lämmastikuvaba sahharoosi juuresolekul;
English: ADD 50 ML OF PHOSPHORIC ACID (D : 1.71) AND 100 ML OF CONCENTRATED HYDROCHLORIC ACID (D : 1.19) ;
Estonian: Lisatakse 50 ml fosforhapet (tihedus 1,71) ja 100 ml kontsentreeritud soolhapet (tihedus 1,19);
English: CONNECT A REFLUX CONDENSER TO THE FLASK , BRING TO THE BOIL AND KEEP THE SOLUTION GENTLY BOILING FOR 10 HOURS.
Estonian: kolb ühendatakse püstjahutiga, lahus kuumutatakse keemiseni ja hoitakse tasasel keemisel 10 tundi.
Latvian: Pievieno kolbai atteces dzesinātāju, uzkarsē līdz viršanai un šķīdumu lēni vāra 10 stundas.
English: THE RELATIVE RF VALUES OF ANTIBIOTICS OF THE TETRACYCLINE GROUP ARE GIVEN BELOW. THESE VALUES MAY VARY SLIGHTLY ACCORDING TO THE QUALITY OF THE PAPER AND ITS MOISTURE CONTENT :
Estonian: Tetratsükliini rühma antibiootikumide suhtelised Rf-väärtused on antud allpool (need väärtused võivad paberi kvaliteedist ja niiskusesisaldusest olenevalt veidi varieeruda):
English: MANSHOLT
Maltese: MANSHOLT
English: TO CHECK THE ACCURACY OF THE METHOD , CARRY OUT THE ANALYSIS (DIGESTION , DISTILLATION AND TITRATION) ON 1.5 TO 2.0 G OF ACETANILIDE A. R. (M. P. 114* C;
Maltese: Biex tivverifika l-preċizjoni tal-metodu, wettaq analiżi (diġestjoni, distillazzjoni u titrazzjoni) fuq 1.5 sa 2.0 g ta' acetanilide A. R. (m. p. 114 °Ċ;
English: APPARATUS FOR ASCENDING PAPER CHROMATOGRAPHY (HEIGHT OF PAPER : 25 CM). SCHLEICHER AND SCHUELL PAPER (2040B OR 2043B) OR EQUIVALENT.
Latvian: Augšupejošās papīra hromatogrāfijas aparāts (papīra augstums 25 cm), Šlaihera (Schleicher) un Šuela (Schuell) papīrs (2040B vai 2043B) vai līdzvērtīgs.
Maltese: Apparat għall-karta tal-kromotografija li tikber (għoli tal-karta: 25 cm). karta Schleicher u Schull (2040b jew 2043b) jew ekwivalenti.
English: USE ELUENT I (3.3) FOR DETECTION BY BIOAUTOGRAPHY AND ELUENT II (3.4) FOR DETECTION BY UV LIGHT. WHEN THE SOLVENT FRONT HAS CLIMBED 15 TO 20 CM (APPROX. 1 HOUR 30 MINUTES) , STOP CHROMATOGRAPHY AND DRY THE PAPER.
Maltese: Uża eluent 1 (3.3) għall-kxif permezz ta' bijoawtografija u eluent 11 (3.4) għall-kxif permezz ta' dawl U.V. Meta l-parti ta' quddiem tas-solvent ikun tela' bejn 15 u 20 ċm (bejn wieħed u ieħor siegħa u 30 minuta) waqqaf il-kromatografija u nixxef il-karta.
English: INCUBATE OVERNIGHT AT 37* C. KEEP THE CULTURE IN A REFRIGERATOR AND RE-INOCULATE SLOPED AGAR WITH IT EVERY 4 WEEKS.
Maltese: Inkuba matul il-lejl f'37 °Ċ. Żomm il-kultura f'refriġeratur u innokula mill-ġdid agar imżerżaq bih kull 4 ġimgħat.
English: 24 HOURS BEFORE USE , RE-INOCULATE SLOPED AGAR WITH A SUB-CULTURE AND INCUBATE OVERNIGHT AT 37* C. SUSPEND ALL THE CULTURE CONTAINED IN A TUBE OF AGAR IN APPROXIMATELY 2 ML OF THE BASIC MEDIUM (4.1) , THEN TRANSFER THE SUSPENSION UNDER STERILE CONDITIONS INTO APPROXIMATELY 100 ML OF THE SAME BASIC MEDIUM (4.1).
Maltese: 24 siegħa qabel l-użu, innokula mill-ġdid agar imżerżaq b'sub-kultura u inkuba matul il-lejl f'37 °Ċ. Dendel il-kulturi kollha li jinsabu f'tubu ta' l-agar f'bejn wieħed u ieħor 2 ml ta' mezz bażiku (4.1), imbagħad ittrasferixxi s-sospensjoni taħt kondizzjonijiet sterili f'bejn wieħed u ieħor 100 ml ta' l-istess mezz bażiku (4.1).
English: MEASURE THE DIAMETER OF THE INHIBITION ZONES , PREFERABLY BY PROJECTION. RECORD THE MEASUREMENTS ON SEMI-LOGARITHMIC PAPER , PLOTTING THE LOGARITHM OF THE CONCENTRATIONS AGAINST THE DIAMETER OF THE INHIBITION ZONES.
Maltese: Kejjel id-dijametru taż-żoni inibiti, preferibbilment bi proġezzjoni. irrekordja l-kejl fuq karta semi-logaritmika, billi tniżżel il-logaritmu tal-konċentrazzjonijiet kontra d-dijametru ta' dawn iż-żoni ta' inibizzjoni.
English: INCUBATE OVERNIGHT AT APPROXIMATELY 35* C. KEEP THE CULTURE IN A REFRIGERATOR AND RE-INOCULATE SLOPED AGAR WITH IT EVERY MONTH.
Maltese: Inkuba matul il-lejl f'bejn wieħed u ieħor 35 °Ċ. Żomm il-kultura f'refriġeratur u innokula mill-ġdid agar imżerżaq biha kull xahar.
English: FOR PREMIXES AND FEEDINGSTUFFS , A 20 G TEST SAMPLE. ADD 60 ML OF PHOSPHATE BUFFER SOLUTION , PH 8 (4.2) , PREVIOUSLY HEATED TO 80* C , AND HOMOGENIZE FOR 2 MINUTES (DOMESTIC MIXER , ULTRA-TURRAX , ETC.).
Maltese: għal taħlitiet minn qabel u għalf, kampjun tat-test ta' 20 g. Żid 60 ml tas-soluzzjoni mewwieta tal-fosfat, pH 8 (4.2), imsaħħna minn qabel għal 80 °Ċ, u omoġenizza għal 2 minuti (apparat domestiku tat-taħlit, Ultra-turrax, ecc.).
English: INCUBATE OVERNIGHT AT APPROXIMATELY 35* C. KEEP THE CULTURE IN A REFRIGERATOR AND RE-INOCULATE SLOPED AGAR WITH IT EVERY 14 DAYS.
Maltese: Inkuba matul il-lejl f'bejn wieħed u ieħor 35 °Ċ. Żomm il-kultura f'refriġeratur u innokula mill-ġdid b'agar imżerżaq miegħu kull 14-il jum.
English: LEAVE TO COOL. ADD A FEW GRANULES OF ZINC (3.3).
Lithuanian: Pridedama keletas cinko granulių (3.3).
English: PREPARATION OF SOLUTION (SEE OBSERVATION 7.2) WEIGH 2 G OF THE SAMPLE TO THE MEAREST MG AND PLACE IN A 500 ML GRADUATED FLASK.
Lithuanian: Tirpalo ruošimas (žr. 7.2 pastabą) 1 mg tikslumu pasveriama 2 g mėginio ir supilama į 500 ml matavimo kolbą.
English: BY VOLUME PREPARE IMMEDIATELY BEFORE USE.
Hungarian: Közvetlenül a felhasználás előtt kell elkészíteni.
Lithuanian: Ruošiama prieš pat naudojimą.
English: INOCULATION OF THE CULTURE MEDIUM INOCULATE THE BASIC MEDIUM FOR THE DETERMINATION (4.1) WITH THE INOCULUM (3.2) TO OBTAIN WITH THE PHOTOMETER AT 590 NM 85 % LIGHT TRANSMISSION IN A 5 CM CELL OR 92 % TRANSMISSION IN A 2 CM CELL , THE APPARATUS BEING SET AT 100 % TRANSMISSION ON THE NON-INOCULATED BASIC MEDIUM (4.1).
Lithuanian: Sėjimas mitybinėje terpėje Į pagrindinę nustatymo mitybinę terpę (4.1) sėjama inokuliantu (3.2), kad šviesos pralaidumo koeficientas, esant 590 nm bangos ilgiui, fotometre su 5 cm storio kiuvete būtų 85 %, su 2 cm storio kiuvete - 92 %, prietaisą nustačius 100 % pralaidumui, kai pagrindinė mitybinė terpė (4.1) neapsėta.
English: DETERMINATION OF OLEANDOMYCIN - BY DIFFUSION ON AGAR -
Hungarian: AZ OLEANDOMICIN MEGHATÁROZÁSA - agardiffúzióval -
Lithuanian: OLEANDOMICINO NUSTATYMAS - matuojant difuziją ant agaro -
English: PREPARATION OF THE SPORE SUSPENSION COLLECT THE BACTERIA FROM A TUBE OF SLOPED AGAR (3.1) USING APPROXIMATELY 3 ML OF PHYSIOLOGICAL SALINE (4.3).
Lithuanian: Sporų suspensijos ruošimas Bakterijos iš vamzdelio su gulsčiuoju agaru (3.1) surenkamos, naudojant maždaug 3 ml fiziologinio tirpalo (4.3).
English: DISTILLED WATER TO 1 000 ML ADJUST THE PH TO 6.5 IMMEDIATELY BEFORE USE.
Lithuanian: Distiliuotas vanduo iki 1000 ml Prieš pat naudojant nustatomas pH 6,5.
English: DISTILLED WATER 50 ML TRI (HYDROXYMETHYLAMINO) METHANE A. R. 0.5 G
Lithuanian: Distiliuotas vanduo 50 ml Tri(hidroksimetilamino)metanas a. r. 0,5 g
English: S4 0.05 UG/ML S2 0.025 UG/ML
Lithuanian: S4 0,05 g/ml S2 0,025 g/ml
English: INOCULATION OF THE CULTURE MEDIUM INOCULATE AT 60* C THE BASIC MEDIUM FOR THE DETERMINATION (4.2) WITH THE SPORE SUSPENSION (3.2).
Lithuanian: Sėjimas į mitybinę terpę Į pagrindinę mitybinę terpę (4.2) 60 °C temperatūroje pasėjama sporų suspensija (3.2).
English: REPEATABILITY THE DIFFERENCE BETWEEN THE RESULTS OF TWO PARALLEL DETERMINATION CARRIED OUT ON THE SAME SAMPLE MUST NOT EXCEED 10 % IN RELATIVE VALUE.
Lithuanian: Pakartojamumas Skirtumas tarp dviejų rezultatų, gautų atliekant du lygiagrečius matavimus su tuo pačiu mėginiu, santykine verte turi neviršyti 10 %.
English: DETERMINATION OF TYLOSIN - BY DIFFUSION ON AGAR -
Hungarian: A TILOZIN MEGHATÁROZÁSA - agardiffúzióval -
Lithuanian: TILOZINO NUSTATYMAS - matuojant difuziją ant agaro -
English: MICRO-ORGANISM : SARCINA LUTEA ATCC NO 9341 3.
Lithuanian: Mikroorganizmas: Sarcina lutea ATCC Nr. 9341
English: 1 MAINTENANCE OF THE PARENT
Lithuanian: Pradinio štamo laikymas
English: STRAIN INOCULATE WITH SARCINA LUTEA A TUBE OF SLOPED AGAR TAKEN FROM THE CULTURE MEDIUM (4.1) , ADJUST TO PH 7.0.
Lithuanian: Vamzdelyje su gulsčiuojuagaru, paimtu iš mitybinės terpės (4.1), pasėjama Sarcina lutea ir nustatoma pH 7,0.
English: STANDARD SOLUTIONS DRY
Lithuanian: Etaloniniai tirpalai
English: THE STANDARD SUBSTANCE (4.8) FOR 3 HOURS AT 60* C IN A VACUUM OVEN (5 MM OF MERCURY).
Lithuanian: Etaloninė medžiaga (4.8) tris valandas 60 °C temperatūroje džiovinama vakuuminiame eksikatoriuje (slėgis 5 mm gyvsidabrio stulpelio).
English: THEN PREPARE BY SUCCESSIVE DILUTIONS (1 + 1) , USING THE MIXTURE (4.7) , THE FOLLOWING CONCENTRATIONS : S4 1 UG/ML
Lithuanian: Nuosekliai praskiedžiant (1: 1) mišiniu (4.7), paruošiami tokių koncentracijų tirpalai: S4 1,0 g/ml
English: S2 0.5 UG/ML S1 0.25 UG/ML
Lithuanian: S2 0,5 g/ml S1 0,25 g/ml
English: INOCULATION OF THE CULTURE MEDIUM INOCULATE AT 48 TO 50* C THE BASIC MEDIUM FOR THE DETERMINATION (4.1) , ADJUST TO PH 8.0 , WITH THE BACTERIA SUSPENSION (3.2).
Lithuanian: Sėjimas į mitybinę terpę Į pagrindinę nustatymo mitybinę terpę (4.1), nustačius pH 8,0, esant 48-50 °C temperatūrai pasėjama bakterijų suspensija (3.2).
English: DETERMINATION OF VIRGINIAMYCIN - BY DIFFUSION ON AGAR -
Hungarian: A VIRGINIAMICIN MEGHATÁROZÁSA - agardiffúzióval -
Lithuanian: VIRGINIAMICINO NUSTATYMAS - matuojant difuziją ant agaro -
English: MAINTENANCE OF THE PARENT STRAIN INOCULATE WITH S. LUTEA A TUBE OF SLOPED AGAR TAKEN FROM THE CULTURE MEDIUM (4.1).
Lithuanian: Pradinio štamo laikymas Vamzdelyje su gulsčiuojuagaru, paimtu iš mitybinės terpės (4.1), sėjama Sarcina lutea.
English: PREPARATION OF THE BACTERIA SUSPENSION COLLECT THE BACTERIA FROM A RECENTLY PREPARED TUBE OF SLOPED AGAR (3.1) USING 2 TO 3 ML OF PHYSIOLOGICAL SALINE (4.3).
Lithuanian: Bakterijų suspensijos ruošimas Bakterijos iš vamzdelio su neseniai paruoštu gulsčiuoju agaru (3.1) surenkamos, naudojant 2-3 ml fiziologinio tirpalo (4.3).
English: HOMOGENIZE , AND DILUTE THIS SUSPENSION TO OBTAIN APPROXIMATELY 75 % LIGHT TRANSMISSION AT 650 NM. I
Lithuanian: Ši suspensija homogenizuojama ir praskiedžiama tiek, kad šviesos pralaidumo koeficientas esant 650 nm bangos ilgiui būtų apie 75 %.
English: F KEPT IN A REFRIGERATOR THIS SUSPENSION MAY BE USED FOR ONE WEEK.
Lithuanian: Šaldytuve laikoma ši suspensija gali būti naudojama vieną savaitę.
English: THEN PREPARE BY SUCCESSIVE DILUTIONS (1 + 1) , USING THE MIXTURE (4.5) , THE FOLLOWING CONCENTRATIONS : S4 0.5 UG/ML S2 0.25 UG/ML S1 0.125 UG/ML
Lithuanian: Nuosekliai praskiedžiant (1: 1) mišiniu (4.5), paruošiami tokių koncentracijų tirpalai: S4 0,5 g/ml S2 0,25 g/ml S1 0,125 g/ml
English: PRODUCTS WITH A VIRGINIAMYCIN CONTENT OF 50 PPM OR LESS TAKE A TEST SAMPLE OF 10 TO 20 G , ADD 100 ML OF THE SOLUTION (4.6) AND SHAKE FOR 30 MINUTES ON A SHAKING PLATFORM.
Lithuanian: Produktai, kuriuose virginiamicino koncentracija 50 ppm arba mažesnė Paimama 10-20 g tiriamojo mėginio, įpilama 100 ml tirpalo (4.6) ir 30 minučių purtoma ant purtyklės.
English: PRODUCTS WITH A VIRGINIAMYCIN CONTENT GREATER THAN 50 PPM TAKE A TEST SAMPLE OF 1 TO 10 G , ADD 100 ML OF SOLUTION (4.6) AND SHAKE FOR 30 MINUTES ON A SHAKING PLATFORM.
Lithuanian: Produktai, kuriuose virginiamicino koncentracija didesnė kaip 50 milijonųjų dalių Paimama 1-10 g tiriamojo mėginio, įpilama 100 ml tirpalo (4.6) ir purtoma 30 minučių ant purtyklės.
English: 40 % (V/V) ETHANOL , D : 0.948 AT 20o C.
Latvian: 40 % (tilp./tilp.) etilspirts, d = 0,948 pie 20 oC.
English: AFTER EXACTLY 15 MINUTES , REMOVE FROM THE BATH , ADD 30 ML OF COLD WATER AND COOL IMMEDIATELY TO 20o C.
Latvian: Precīzi pēc 15 minūtēm kolbu izņem no vannas, pievieno 30 ml auksta ūdens un tūlīt atdzesē līdz 20 oC.
English: AFTER EXACTLY 15 MINUTES , REMOVE THE ERLENMEYER FLASK FROM THE BATH , TRANSFER THE CONTENTS TO A 100 ML GRADUATED FLASK , RINSING WITH A LITTLE COLD WATER , AND COOL TO 20o C.
Latvian: Precīzi pēc 15 minūtēm izņem konisko kolbu no ūdens vannas, saturu pārnes 100 ml mērkolbā, skalojot ar nelielu daudzumu auksta ūdens, un atdzesē līdz 20 oC.
English: FIT A REFLUX CONDENSER TO THE FLASK AND IMMERSE THE LATTER IN A WATER BATH AT 50o C FOR 30 MINUTES.
Latvian: Koniskajai kolbai uzliek atteces dzesinātāju un kolbu uz 30 minūtēm iegremdē ūdens vannā pie 50 oC.
English: TO CHECK THE ACCURACY OF THE METHOD , CARRY OUT THE ANALYSIS (DIGESTION , DISTILLATION AND TITRATION) ON 1.5 TO 2.0 G OF ACETANILIDE A. R. IN THE PRESENCE OF 1 G OF NITROGEN-FREE SUCROSE;
Latvian: Lai pārbaudītu metodes precizitāti, izdara analīzi (šķelšanu, destilēšanu un titrēšanu) ar 1,5 - 2,0 g analīzes kvalitātes acetanilīda 1 g slāpekli nesaturošas saharozes klātbūtnē.
English: THE SAMPLE IS HEATED FOR 48 HOURS AT 40o C IN A SOLUTION OF PEPSIN HYDROCHLORIDE.
Latvian: Paraugu 48 stundas silda pie 40 oC pepsīna sālsskābes šķīdumā.
English: WATER BATH OR INCUBATOR , SET AT 40o C MORE OR LESS 1o C.
Latvian: Ūdens vanna vai inkubators, noregulēts pie 40oC 1 oC.
English: ADD 450 ML OF PEPSIN HYDROCHLORIDE SOLUTION (3.4) PREVIOUSLY HEATED TO 40oC AND SHAKE TO PREVENT THE FORMATION OF AGGLOMERATES.
Latvian: Pievieno 450 ml pepsīna sālsskābes šķīduma (3.4.), kas iepriekš uzsildīts līdz 40 oC, un krata, lai novērstu aglomerātu veidošanos.
English: AFTER 48 HOURS , ADD 15 ML OF HYDROCHLORIC ACID (3.1) , COOL TO 20oC , MAKE UP TO VOLUME WITH WATER AND FILTER.
Latvian: Pēc 48 stundām pievieno 15 ml sālsskābes (3.1.), atdzesē līdz 20 oC, uzpilda līdz zīmei ar ūdeni un nofiltrē.
English: PRODUCTS WITH AN OIL OR FAT CONTENT EXCEEDING 10 % MUST FIRST BE DEFATTED BY EXTRACTION WITH PETROLEUM ETHER (B. P. 40 TO 60oC).
Latvian: Produkti ar eļļas vai tauku saturu, kas pārsniedz 10 %, vispirms ir jāatbrīvo no taukiem, ekstrahējot ar petrolēteri (v.40. lpp. līdz 60 oC).
English: WATER BATH SET AT 25oC MORE OR LESS 0.1oC BY ULTRATHERMOSTAT.
Latvian: Ūdens vanna, noregulēta uz 25 0,1 oC ar ultratermostatu.
English: PIPETTE 5.0 ML OF HAEMOGLOBIN SOLUTION (3.7) INTO A TEST TUBE , HEAT TO 25oC IN THE WATER BATH (4.1) , ADD 1.0 ML OF THE PEPSIN SOLUTION OBTAINED IN 5.1 AND MIX WITH A GLASS ROD THICKENED AT ONE END , WITH ABOUT 10 BACK-AND-FORTH MOVEMENTS.
Latvian: Iepipetē mēģenē 5,0 ml hemoglobīna šķīduma (3.7.), uzsilda ūdens vannā līdz 25 oC, pievieno 1,0 ml pepsīna šķīduma, kas iegūts atbilstīgi 5.1. punktam, un samaisa ar vienā galā paresninātu stikla spieķīti apmēram ar 10 kustībām uz priekšu un atpakaļ.
English: LEAVE THE TEST TUBE IN THE WATER BATH AT 25oC FOR EXACTLY 10 MINUTES , TIMED FROM THE ADDITION OF THE PEPSIN SOLUTION (DURATION AND TEMPERATURE MUST BE STRICTLY OBSERVED).
Latvian: Atstāj mēģeni ūdens vannā pie 25 oC precīzi 10 minūtes, skaitot no pepsīna šķīduma pievienošanas (ilgums un temperatūra ir stingri jāievēro).
English: THEN ADD 10.0 ML OF TRICHLOROACETIC ACID SOLUTION (3.4) PREVIOUSLY HEATED TO 25oC , HOMOGENIZE AND FILTER THROUGH A DRY FILTER.
Latvian: Pēc tam pievieno 10,0 ml trihloretiķskābes šķīduma (3.4.), kas iepriekš uzsildīts līdz 25 oC, homogenizē un nofiltrē caur sausu filtru.
English: PIPETTE 5.0 ML OF HAEMOGLOBIN SOLUTION (3.7) INTO A TEST TUBE , HEAT TO 25* C IN THE WATER BATH (4.1) , ADD 10.0 ML OF TRICHLOROACETIC ACID SOLUTION (3.4) PREVIOUSLY HEATED TO 25oC , HOMOGENIZE , THEN ADD 1.0 ML OF THE PEPSIN SOLUTION OBTAINED IN 5.1.
Latvian: Iepipetē mēģenē 5,0 ml hemoglobīna šķīduma (3.7.), uzsilda ūdens vannā (4.1.) līdz 25 oC, pievieno 10,0 ml trihloretiķskābes šķīduma (3.4.), kas iepriekš sasildīts līdz 25 oC, homogenizē, pēc tam pievieno 1,0 ml pepsīna šķīduma, kas iegūts saskaņā ar 5.1. punktu.
English: MIX WITH A GLASS ROD AND LEAVE THE TEST TUBE IN THE WATER BATH (4.1) AT 25oC FOR EXACTLY 10 MINUTES.
Latvian: Ar stikla spieķīti samaisa un atstāj mēģeni ūdens vannā (4.1.) precīzi 10 minūtes.
English: TWO UNITS PER MG OBTAINED BY THIS METHOD CORRESPOND TO : 3.64 ANSON MILLIUNITS/MG (UMOLES OF TYROSINE/MG MIN AT 35.5oC) OR 36 400 COMMERCIAL UNITS/G (UMOLES OF TYROSINE/G IN 10 MIN AT 35.5oC).
Latvian: Ar šo metodi iegūtas divas vienības uz mg atbilst 3,64 Ansona milivienībām uz mg (mikromoli tirozīna/mg. min. pie 35,5 oC) vai 36 400 komercvienības/g (mikromoli tirozīna/g 10 minūtēs pie 35,5 oC).
English: THE ANALYSIS SHOULD BE CARRIED OUT AT A ROOM TEMPERATURE OF ABOUT 20oC.
Latvian: Analīze būtu jāizdara istabas temperatūrā - aptuveni 20 oC.
English: PIPETTE IDENTICAL ALIQUOT PARTS OF FILTRATE CONTAINING 50 TO 100 UG OF GOSSYPOL INTO EACH OF TWO 25 ML GRADUATED FLASKS (A AND B). IF NECESSATY , MAKE UP THE VOLUME TO 10 ML WITH SOLVENT A (3.2).
Latvian: Iepipetē identiskas filtrāta alikvotās daļas, kas satur 50 g līdz 100 g gosipola, katrā no divām 25 ml mērkolbām (A un B), Vajadzības gadījumā tilpumu uzpilda līdz 10 ml ar šķīdinātāju A (3.2.).
English: MIXTURE OF PURE NITROMETHANE/PURE CHLOROFORM/1 , 3-DICHLOROPROPAN-2-OL : 20/10/1.5 BY VOLUME PREPARE IMMEDIATELY BEFORE USE.
Latvian: Tīra nitrometāna/tīra hloroforma/1,3-dihlor-2-propanola maisījums -20/10/1,5 tilpuma daļas - jāsagatavo tieši pirms lietošanas.
English: INCUBATOR SET AT 30oC.
Latvian: Inkubators, ieregulēts uz 30 oC.
English: THE INCUBATE OVERNIGHT AT 30oC.
Latvian: Inkubē vienu nakti pie 30 oC.
English: INCUBATE OVERNIGHT AT APPROXIMATELY 3oC.
Latvian: inkubē vienu nakti aptuveni 30 oC.
English: INCUBATE FOR 3 TO 5 DAYS AT 28 TO 30oC , THEN COLLECT THE SPORES IN 15 ML OF ETHANOL (4.6) AFTER CHECKING SPORULATION UNDER A MICROSCOPE , AND HOMOGENIZE.
Latvian: Inkubē 3 - 5 dienas pie 28 - 30 oC, sporu veidošanos pārbauda mikroskopā, pēc tam savāc sporas 15 mililitros etilspirta (4.6.) un homogenizē.
English: THE CULTURE MEDIUM IS INOCULATED AT BETWEEN 50 AND 60oC.
Latvian: Barotni apsēj temperatūrā starp 50 un 60 oC.
English: INOCULATE AT 50 TO 60oC THE BASIC MEDIUM FOR THE DETERMINATION (4.1) WITH THE SPORE SUSPENSION (3.2).
Latvian: Noteikšanai 50 - 60 oC temperatūrā pamatbarotni (4.1.) apsēj ar sporu suspensiju (3.2.).
English: INCUBATE THE TRAYS FOR APPROXIMATELY 18 HOURS AT 28 TO 30oC.
Latvian: Paplātes inkubē aptuveni 18 stundas pie 28 - 30 oC.
English: INCUBATE OVERNIGHT AT 37oC.
Latvian: inkubē vienu nakti 37 oC temperatūrā.
English: 24 HOURS BEFORE USE , RE-INOCULATE SLOPED AGAR WITH A SUB-CULTURE AND INCUBATE OVERNIGHT AT 37oC.
Latvian: 24 stundas pirms lietošanas slīpi novietoto agaru apsēj ar subkultūru un inkubē vienu nakti pie 37 oC.
English: INCUBATE IN A WATER BATH AT 37oC UNTIL THE GROWTH OF THE STRAIN ENTERS ITS LOGARITHMIC PHASE (1 HOUR 30 MINUTES TO 2 HOURS).
Latvian: Ūdens vannā inkubē pie 37 oC, līdz klona augšana ieiet logaritmiskā fāze (1 stunda 30 minūtes līdz 2 stundām)3.
English: INCUBATION MUST BE CARRIED OUT IN A WATER BATH WHOSE TEMPERATURE IS KEPT UNIFORM AT 37oC MORE OR LESS 0.1oC BY STIRRING.
Latvian: Inkubācija jāizdara ūdens vannā, kuras temperatūru, maisot, uztur nemainīgu - 37 0,1 oC.
English: INCUBATE OVERNIGHT AT APPROXIMATELY 30oC. KEEP THE CULTURE IN A REFRIGERATOR AND RE-INOCULATE SLOPED AGAR WITH IT EVERY 4 WEEKS.
Latvian: Kultūru glabā ledusskapī un slīpi novietoto agaru no jauna ar to apsēj reizi 4 nedēļās.
English: INCUBATE FOR 3 TO 5 DAYS AT 28 TO 30oC , THEN COLLECT THE SPORES IN 15 ML OF ETHANOL (4.4) AFTER CHECKING SPORULATION UNDER A MICROSCOPE , AND HOMOGENIZE.
Latvian: Inkubē 3 līdz 5 dienas pie 28 - 30 oC, sporu veidošanos pārbauda mikroskopā, pēc tam savāc sporas 15 mililitros etilspirta (4.4.) un homogenizē.
English: THE CULTURE MEDIUM IS INOCULATED AT 60oC.
Latvian: Barotni apsēj 60 oC temperatūrā.
English: INOCULATE AT 60oC THE BASIC MEDIUM FOR THE DETERMINATION (4.2) WITH THE SPORE SUSPENSION (3.2).
Latvian: Noteikšanas pamatbarotni (4.2.) 60 oC temperatūrā apsēj ar sporu suspensiju (3.2.).
English: THE SAMPLE IS TREATED WITH A PH 8 PHOSPHATE BUFFER SOLUTION , PREVIOUSLY HEATED TO 80oC , AND THEN EXTRACTED WITH METHANOL.
Latvian: Paraugu apstrādā ar pH 8 fosfāta buferšķīdumu, kas iepriekš uzkarsēts līdz 80 oC, un pēc tam ekstrahē ar metilspirtu.
English: INCUBATE OVERNIGHT AT APPROXIMATELY 35oC.
Latvian: inkubē vienu nakti aptuveni 35 oC temperatūrā.