FrancophonIA/DGT-Translation_Memory huggingface dataset:
English: INCUBATE OVERNIGHT AT APPROXIMATELY 30* C. KEEP THE CULTURE IN A REFRIGERATOR AND RE-INOCULATE SLOPED AGAR WITH IT EVERY 4 WEEKS.
Czech: Skladovat kulturu v chladničce a přeočkovat každé 4 týdny na šikmý agar.
Maltese: Inkuba matul il-lejl f'bejn wieħed u ieħor 30 °Ċ. Żomm il-kultura f'refriġeratur u innokula mill-ġdid biha l-agar imżerżaq kull 4 ġimgħat.
English: THE TEST SHOULD PREFERABLY BE CARRIED OUT ON FLAT TRAYS CONSISTING OF GLASS PLATES FITTED WITH A PERFECTLY LEVEL ALUMINIUM OR PLASTIC RING.
Czech: Upřednostňuje se použití misek sestávajících se z plochých skleněných desek s dokonale rovným hliníkovým nebo plastovým kroužkem
Latvian: Analīzi vēlams izdarīt uz paplātēm, kas sastāv no plakana stikla plāksnēm, kas aprīkotas ar pilnīgi horizontālu alumīnija vai plastmasas gredzenu,
English: 200 MM IN DIAMETER AND 20 MM HIGH.
Czech: o průměru 200 mm a o výšce 20 mm.
Latvian: kuram ir 200 mm diametrs un 20 mm augstums.
English: HAVING REGARD TO THE COUNCIL DIRECTIVE OF 20 JULY 1970 ON THE INTRODUCTION OF COMMUNITY METHODS OF SAMPLING AND ANALYSIS FOR THE OFFICIAL CONTROL OF FEEDINGSTUFFS, AND IN PARTICULAR ARTICLE 2 THEREOF;
Slovenian: ob upoštevanju Direktive Sveta z dne 20. julija 1970 o uvedbi metod vzorčenja in analiz Skupnosti za uradni nadzor krme in zlasti člena 2 Direktive,
English: WHEREAS THAT DIRECTIVE REQUIRES THAT OFFICIAL CONTROLS OF FEEDINGSTUFFS BE CARRIED OUT USING COMMUNITY METHODS OF SAMPLING AND ANALYSIS FOR THE PURPOSE OF CHECKING COMPLIANCE WITH REQUIREMENTS ARISING UNDER THE PROVISIONS LAID DOWN BY LAW, REGULATION OR ADMINISTRATIVE ACTION CONCERNING THE QUALITY AND COMPOSITION OF FEEDINGSTUFFS;
Slovenian: ker navedena direktiva zahteva, da se uradni nadzor krme izvaja na podlagi metod vzorčenja in analiz Skupnosti za preverjanje skladnosti z zahtevami, določenimi z zakoni in drugimi predpisi v zvezi s kakovostjo in sestavo krme;
English: WHEREAS COMMISSION DIRECTIVES NO 71/250/EEC OF 15 JUNE 1971 AND NO 71/393/EEC OF 18 NOVEMBER 1971 HAVE ALREADY ESTABLISHED A NUMBER OF COMMUNITY METHODS OF ANALYSIS;
Slovenian: ker direktivi Komisije št. 71/250/EGS/EGS z dne 15. junija 1971 in št. 71/393/EGS z dne 18. novembra 1971 že določata številne metode analiz Skupnosti;
English: WHEREAS, IN VIEW OF PROGRESS MADE IN SUBSEQUENT WORK, A THIRD SET OF METHODS SHOULD BE ADOPTED;
Slovenian: ker je treba glede na napredek, ki je bil dosežen v nadaljnjem delu, sprejeti tretji sklop metod;
English: HAS ADOPTED THIS DIRECTIVE:
Slovenian: SPREJELA NASLEDNJO DIREKTIVO:
English: THE MEMBER STATES SHALL REQUIRE THAT ANALYSES FOR OFFICIAL CONTROLS OF FEEDINGSTUFFS AS REGARDS THEIR LEVELS OF STARCH, CRUDE PROTEIN, CRUDE PROTEIN WHICH CAN BE DISSOLVED BY PEPSIN AND HYDROCHLORIC ACID, OF FREE AND TOTAL GOSSYPOL AND AS REGARDS PEPSIN ACTIVITY BE CARRIED OUT USING THE METHODS DESCRIBED IN ANNEX I TO THIS DIRECTIVE.
Slovenian: Države članice zahtevajo, da se uradni nadzor krme glede vsebnosti škroba, surovih beljakovin, surovih beljakovin, topnih v pepsinu in klorovodikovi kislini, prostega in celotnega gosipola in aktivnosti pepsina izvajajo z metodami, opisanimi v Prilogi I k tej direktivi.
English: THE MEMBER STATES SHALL REQUIRE THAT ANALYSES FOR OFFICIAL CONTROLS OF FEEDINGSTUFFS, FOR THE PURPOSE OF DETECTING AND IDENTIFYING ANTIBIOTICS OF THE TETRACYCLINE GROUP AND ALSO FOR DETERMINING THE LEVELS OF CHLORETRACYCLINE, OXYTETRACYCLINE, TETRACYCLINE, OLEANDOMYCIN, TYLOSIN, AND VIRGINIAMYCIN IN FEEDINGSTUFFS, BE CARRIED OUT USING THE METHODS DESCRIBED IN ANNEX II TO THIS DIRECTIVE.
Slovenian: Države članice zahtevajo, da se analize za uradni nadzor krme, katerih namen je odkriti in identificirati antibiotike skupine tetraciklinov ter določiti vsebnost klortetraciklina, oksitetraciklina, tetraciklina, oleandomicina, tilozina in virginiamicina v krmi, izvajajo z metodami, ki so opisane v Prilogi II k tej direktivi.
English: THE GENERAL PROVISIONS SET OUT IN PART 1 (INTRODUCTION) OF THE ANNEX TO THE FIRST COMMISSION DIRECTIVE NO 71/250/EEC OF 15 JUNE 1971, WITH THE EXCEPTION OF THOSE CONCERNING THE PREPARATION OF THE SAMPLE FOR ANALYSIS, SHALL BE APPLICABLE TO THE METHODS DESCRIBED IN ANNEX II TO THIS DIRECTIVE.
Slovenian: Splošne določbe, navedene v delu I (Uvod) Priloge k Prvi direktivi Komisije št. 71/250/EGS z dne 15. junija 1971, razen tistih, ki določajo pripravo vzorca za analizo, veljajo za metode, ki so opisane v Prilogi II k tej direktivi.
English: THE MEMBER STATES SHALL, NOT LATER THAN 1 JULY 1973, BRING INTO FORCE THE LAWS, REGULATIONS OR ADMINISTRATIVE PROVISIONS NECESSARY TO COMPLY WITH THIS DIRECTIVE.
Slovenian: Države članice sprejmejo zakone in druge predpise, potrebne za uskladitev s to direktivo, najpozneje do 1. julija 1973.
English: DONE AT BRUSSELS, 27 APRIL 1972.
Slovenian: V Bruslju, 27. aprila 1972
English: THIS METHOD MAKES IT POSSIBLE TO DETERMINE THE LEVELS OF STARCH AND OF HIGH MOLECULAR WEIGHT STARCH DEGRADATION PRODUCTS IN FEEDINGSTUFFS, WITH THE EXCEPTION OF THOSE FEEDINGSTUFFS WHICH CONTAIN BEET CHIPS, BEET PULP, DRIED BEET TOPS OR LEAVES, POTATO PULP, DEHYDRATED YEAST, PRODUCTS RICE IN INULIN (E. G. CHIPS AND MEAL OF JERUSALEM ARTICHOKES) OR GREAVES.
Slovenian: S to metodo je mogoče določiti raven škroba in raven razkrojnih produktov škroba z visoko molekulsko maso v krmi, razen v tisti krmi, ki vsebuje koščke ali pulpo repe, posušene repne glave ali liste, krompirjevo pulpo, suhi kvas, proizvode z visoko vsebnostjo inulina (npr. koščke in moko topinamburja) ali ocvirkov.
English: IN THE FIRST, THE SAMPLE IS TREATED WHEN HOT WITH DILUTE HYDROCHLORIC ACID.
Slovenian: Pri prvem vzorec obdelamo z razredčeno klorovodikovo kislino, ko je vroč.
English: IN THE SECOND, THE SAMPLE IS EXTRACTED WITH 40 % ETHANOL.
Slovenian: Pri drugem vzorec ekstrahiramo s 40-odstotnim etanolom.
English: AFTER ACIDIFYING THE FILTRATE WITH HYDROCHLORIC ACID, CLARIFYING AND FILTERING, THE OPTICAL ROTATION IS MEASURED AS IN THE FIRST DETERMINATION.
Slovenian: Po obdelavi filtrata s klorovodikovo kislino, po zbistritvi in filtraciji izmerimo optično rotacijo kakor pri prvem določanju.
English: THE DIFFERENCE BETWEEN THE TWO MEASUREMENTS, MULTIPLIED BY A KNOWN FACTOR, GIVES THE STARCH CONTENT OF THE SAMPLE.
Slovenian: Rezultat razlike med obema meritvama, pomnožene z znanim faktorjem, je vsebnost škroba v vzorcu.
English: 25 % (W/W) HYDROCHLORIC ACID, D: 1.126.
Slovenian: 25 % (m/m) klorovidikova kislina, d: 1,126.
English: 1,128 % (W/V) HYDROCHLORIC ACID.
Slovenian: 1,128 % (m/v) klorovodikova kislina.
English: CARREZ SOLUTION I:
Slovenian: Raztopina Carrez I:
English: CARREZ SOLUTION II:
Slovenian: Raztopina Carrez II:
English: 40 % (V/V) ETHANOL, D: 0.948 AT 20* C.
Slovenian: 40 % (v/v) etanola, d: 0,948 pri 20 °C.
English: DETERMINATION OF THE TOTAL OPTICAL ROTATION (P OR S)
Slovenian: Določanje skupne optične rotacije (P ali S)
English: ADD 25 ML OF HYDROCHLORIC ACID, SHAKE TO OBTAIN EVEN DISTRIBUTION OF THE TEST SAMPLE AND ADD A FURTHER 25 ML OF HYDROCHLORIC ACID .
Slovenian: Dodamo 25 ml klorovodikove kisline in stresamo, da se preskusni vzorec enakomerno porazdeli. Nato dodamo še 25 ml klorovodikove kisline.
English: AFTER EXACTLY 15 MINUTES, REMOVE FROM THE BATH, ADD 30 ML OF COLD WATER AND COOL IMMEDIATELY TO 20* C.
Slovenian: Po natanko 15 minutah jo vzamemo iz kopeli, dodamo 30 ml mrzle vode in jo takoj ohladimo na 20 °C.
English: ADD 5 ML OF CARREZ SOLUTION I AND SHAKE FOR ONE MINUTE.
Slovenian: Nato dodamo 5 ml raztopine Carrez I in eno minuto stresamo.
English: THEN ADD 5 ML OF CARREZ SOLUTION II AND SHAKE AGAIN FOR ONE MINUTE.
Slovenian: Zatem dodamo 5 ml raztopine Carrez II in spet eno minuto stresamo.
English: MAKE UP TO VOLUME WITH WATER, HOMOGENIZE AND FILTER.
Slovenian: Prilijemo toliko vode, da sega tekočina do oznake, nato jo homogeniziramo in filtriramo.
English: IF THE FILTRATE IS NOT PERFECTLY CLEAR (WHICH IS RARE), REPEAT THE DETERMINATION USING A LARGER QUANTITY OF CARREZ SOLUTIONS I AND II, FOR EXAMPLE 10 ML.
Slovenian: Če filtrat ni povsem bister (kar se zgodi redkokdaj), določanje ponovimo, toda tokrat uporabimo večjo količino raztopin Carrez I in II, na primer 10 ml.
English: WEIGH 5 G OF THE SAMPLE TO THE NEAREST MG, PLACE IN A 100 ML GRADUATED FLASK AND ADD ABOUT 80 ML OF ETHANOL
Slovenian: Natehtamo 5 g vzorca na mg natančno, ga prenesemo v 100-mililitrsko merilno bučko in dodamo okrog 80 ml etanola
English: DURING THIS TIME, SHAKE VIGOROUSLY ON SIX OCCASIONS SO THAT THE TEST SAMPLE IS THOROUGHLY MIXED WITH THE ETHANOL.
Slovenian: Medtem jo šestkrat močno pretresemo, da se preskusni vzorec dobro premeša z etanolom.
English: MAKE UP TO VOLUME WITH ETHANOL, HOMOGENIZE AND FILTER.
Slovenian: Prilijemo toliko etanola, da sega tekočina do oznake, nato jo homogeniziramo in filtriramo.
English: PIPETTE 50 ML OF THE FILTRATE (= 2.5 G OF THE SAMPLE) INTO A 250 ML ERLENMEYER FLASK, ADD 2.1 ML OF HYDROCHLORIC ACID AND SHAKE VIGOROUSLY.
Slovenian: S kapalko odmerimo 50 ml filtrata (= 2.5 g vzorca) v 250 ml erlenmajerico, dodamo 2,1 ml klorovodikove kisline in jo močno stresemo.
English: AFTER EXACTLY 15 MINUTES, REMOVE THE ERLENMEYER FLASK FROM THE BATH, TRANSFER THE CONTENTS TO A 100 ML GRADUATED FLASK, RINSING WITH A LITTLE COLD WATER, AND COOL TO 20* C.
Slovenian: Po natanko 15 minutah vzamemo erlenmajerico iz kopeli, vsebino prenesemo v 100 ml merilno bučko, ki jo prelijemo z malce mrzle vode, in ohladimo na 20 °C.
English: CLARIFY USING CARREZ SOLUTIONS I AND II, MAKE UP TO VOLUME WITH WATER, HOMOGENIZE, FILTER AND MEASURE THE OPTICAL ROTATION AS INDICATED IN THE 2ND AND 3RD PARAGRAPHS OF 5.2.
Slovenian: Tekočino zbistrimo z raztopinama Carrez I in II, dolijemo vodo, da sega tekočina do oznake, jo homogeniziramo, filtriramo in izmerimo optično rotacijo, kakor navajata drugi in tretji odstavek 5.2.
English: IF THE SAMPLE CONTAINS MORE THAN 6 % OF CARBONATES, CALCULATED IN TERMS OF CALCIUM CARBONATE, THEY MUST BE DESTROYED BY TREATMENT WITH AB EXACTLY APPROPRIATE QUANTITY OF DILUTE SULPHURIC ACID BEFORE DETERMINATION OF THE TOTAL OPTICAL ROTATION.
Slovenian: Če vzorec vsebuje več kakor 6 % karbonatov, ki jih štejemo kot kalcijev karbonat, jih moramo obdelati z najustreznejšo količino razredčene žveplove kisline in tako uničiti, preden določimo skupno optično rotacijo.
English: IN THE CASE OF PRODUCTS WITH A HIGH LACTOSE CONTENT, SUCH AS POWDERED MILK SERUM OR SKIMMED MILK POWDER, PROCEED AS FOLLOWS AFTER ADDING 80 ML OF ETHANOL.
Slovenian: Pri proizvodih z visoko vsebnostjo laktoze, kakor je na primer sirotka v prahu ali posneto mleko v prahu, dodamo 80 ml etanola in nadaljujemo na naslednji način.
English: THIS METHOD MAKES IT POSSIBLE TO DETERMINE CONVENTIONALLY THE CRUDE PROTEIN CONTENT OF FEEDINGSTUFFS ON THE BASIS OF THE NITROGEN CONTENT, DETERMINED ACCORDING TO THE KJELDAHL METHOD.
Slovenian: S to metodo je mogoče po splošno veljavnih pravilih določiti vsebnost beljakovin v krmi, na podlagi vsebnosti dušika, ki se določi po Kjeldahlovi metodi.
English: THE AMMONIA RELEASED IS REMOVED BY DISTILLATION AND COLLECTED IN A MEASURED QUANTITY OF SULPHURIC ACID, THE EXCESS OF WHICH IS TITRATED WITH A SOLUTION OF SODIUM HYDROXIDE.
Slovenian: Z destilacijo ločimo sproščeni amonijak in ga prenesemo v odmerjeno količino žveplove kisline, katere presežek se titrira z raztopino natrijevega hidroksida.
English: CATALYST:
Slovenian: Katalizator:
English: SULPHURIC ACID A. R., D: 1.84.
Slovenian: Žveplov sulfat p.a., d: 1,84.
English: METHYL RED INDICATOR:
Slovenian: Metilno rdeči indikator:
English: 8 % SOLUTION (W/V) OF SODIUM THIOSULPHATE, NA2S2O3.5H2O A. R.
Slovenian: 8-odstotna (m/v) raztopina natrijevega tiosulfata, Na2S2O3 × 5H2O p.a.
English: GRANULATED PUMICE STONE, WASHED IN HYDROCHLORIC ACID AND ASHED.
Slovenian: Zdrobljeni plovec, opran v klorovodikovi kislini in uprašen.
English: ADD 10 G OF POTASSIUM SULPHATE, AN APPROPRIATE QUANTITY OF CATALYST (0.3 TO 0.4 G OF CUPRIC OXIDE OR 0.9 TO 1.2 G OF CUPRIC SULPHATE OR A DROP OF MERCURY OR 0.6 TO 0.7 G OF MERCURIC OXIDE), 25 ML OF SULPHURIC ACID AND A FEW GRANULES OF PUMICE STONE.
Slovenian: Dodamo 10 g kalijevega sulfata, primerno količino katalizatorja (od 0,3 do 0,4 g bakrovega oksida, ali od 0,9 do 1,2 g bakrovega sulfata, ali kapljico živega srebra, ali od 0,6 do 0,7 g živosrebrovega oksida), 25 ml žveplene kisline in nekaj zrnc plovca.
English: HEAT THE FLASK MODERATELY AT FIRST, SHAKING FROM TIME TO TIME, UNTIL THE MASS HAS CARBONIZED AND THE FOAM HAS DISAPPEARED;
Slovenian: Bučko najprej zmerno segrevamo in jo od časa do časa pretresemo, dokler se masa ne karbonizira in pena ne izgine;
English: WHEN THE SOLUTION BECOMES CLEAR AND COLOURLESS (OR LIGHT GREEN IF A COPPER-BASED CATALYST IS USED), CONTINUE TO BOIL FOR ANOTHER HOUR, THEN LEAVE TO COOL.
Slovenian: Ko se raztopina zbistri in postane brezbarvna (ali svetlo zelena, če smo uporabili katalizator, ki vsebuje baker), pustimo, da vre še eno uro, in nato jo ohladimo.
English: CAREFULLY ADD 250 TO 350 ML OF WATER, STIRRING ALL THE WHILE TO DISSOLVE THE SULPHATES COMPLETELY;
Slovenian: Previdno dodamo od 250 do 350 ml vode in pri tem ves čas mešamo, da se sulfati povsem raztopijo;
English: ADD A FEW GRANULES OF ZINC.
Slovenian: Dodamo nekaj zrnc cinka.
English: PLACE IN THE COLLECTING FLASK OF THE DISTILLATION APPARATUS AN EXACTLY MEASURED QUANTITY OF 25 ML OF SULPHURIC ACID 0.1 N OR 0.5 N DEPENDING ON THE PRESUMED NITROGEN CONTENT (SEE OBSERVATION 7.2), AND ADD A FEW DROPS OF METHYL RED INDICATOR.
Slovenian: V zbirno bučko naprave za destiliranje dodamo natanko 25 ml žveplene kisline 0,1 N ali 0,5 N, odvisno od pričakovane vsebnosti dušika (glej opombo 7.2), in dodamo nekaj kapljic metilno rdečega indikatorja.
English: SLOWLY POUR 100 ML OF 40 % SODIUM HYDROXIDE SOLUTION INTO THE FLASK THROUGH THE DROPPING FUNNEL.
Slovenian: Skozi kapalni lijak počasi prilijemo v bučko 100 ml 40-odstotne raztopine natrijevega hidroksida.
English: IF A MERCURY-BASED CATALYST HAS BEEN USED, ALSO ADD EITHER 10 ML OF SODIUM SULPHIDE SOLUTION, OR 25 ML OF SODIUM THIOSULPHATE SOLUTION.
Slovenian: Če smo uporabili katalizator, ki vsebuje živo srebro, dodamo tudi 10 ml raztopine natrijevega sulfida ali pa 25 ml raztopine natrijevega tiosulfata.
English: AT THE END OF THIS TIME, CHECK THE PH OF THE RESULTING DISTILLATE WITH LITMUS PAPER.
Slovenian: Po teh 30 minutah z lakmusovim papirjem preverimo pH vrednost pridobljenega destilata.
English: IF THE REACTION IS ALKALINE, CONTINUE DISTILLATION.
Slovenian: Če je reakcija alkalna, destilacijo nadaljujemo.
English: IF THE LIQUID TURNS YELLOW, IMMEDIATELY ADD AN EXACTLY MEASURED VOLUME OF SULPHURIC ACID 0.1 N OR 0.5 N.
Slovenian: Če se tekočina obarva rumeno, nemudoma dodamo natanko odmerjeno količino žveplove kisline 0,1 N ali 0,5 N.
English: IN THE COLLECTING FLASK TITRATE THE EXCESS SULPHURIC ACID WITH SODIUM HYDROXIDE SOLUTION 0.1 N OR 0.25 N, DEPENDING ON THE NORMALITY OF THE SULPHURIC ACID USED, UNTIL THE COLOUR TURNS PALE YELLOW.
Slovenian: V zbirni bučki titriramo presežek žveplene kisline z raztopino natrijevega hidroksida 0,1 N ali 0,25 N, odvisno od normalnosti uporabljene žveplove kisline, dokler tekočina ne postane bledo rumena.
English: TO ESTABLISH WHETHER THE REAGENTS ARE FREE FROM NITROGEN, CARRY OUT A BLANK TEST (DISTILLATION AND TITRATION) OMITTING THE SAMPLE TO BE ANALYZED.
Slovenian: S slepim preskusom (destilacijo in titracijo) ugotovimo, ali so reagenti brez dušika, toda preskusa ne izvajamo na vzorcu, ki ga analiziramo.
English: TO CHECK THE ACCURACY OF THE METHOD, CARRY OUT THE ANALYSIS (DIGESTION, DISTILLATION AND TITRATION) ON 1.5 TO 2.0 G OF ACETANILIDE A. R. (M. P. 114* C; % N: 10.36) IN THE PRESENCE OF 1 G OF NITROGEN-FREE SUCROSE;
Slovenian: Natančnost te metode preverimo z analizo (razklop, destilacija in titracija) na 1,5 do 2,0 g acetanilida p.a. (tališče: 114°C; % N: 10,36) z 1 g saharoze brez dušika.
English: THE DIFFERENCE BETWEEN THE RESULTS OF TWO PARALLEL DETERMINATIONS CARRIED OUT ON THE SAME SAMPLE MUST NOT EXCEED:
Slovenian: Razlika med rezultatoma dveh vzporednih določitev, izvedenih na istem vzorcu, ne sme presegati:
English: 0.2, IN ABSOLUTE VALUE, FOR CRUDE PROTEIN CONTENTS OF LESS THAN 20 %;
Slovenian: 0,2, v absolutni vrednosti, za vsebnosti surovih beljakovin pod 20 %;
English: %, IN RELATIVE VALUE, FOR CONTENTS OF NOT LESS THAN 20 % AND NOT MORE THAN 40 %;
Slovenian: 1,0 %, v relativni vrednosti, za vsebnosti, ki niso manjše od 20 % in ne večje od 40 %;
English: 0.4, IN ABSOLUTE VALUE, FOR CONTENTS OF MORE THAN 40 %.
Slovenian: 0,4, v absolutni vrednosti, za vsebnosti nad 40 %.
English: IF SUCH APPARATUS IS USED, THE TRANSFER MUST BE CARRIED OUT WITHOUT LOSS.
Slovenian: Če uporabimo takšno napravo, moramo paziti, da med pretakanjem ne nastane izguba.
English: FOR PRODUCTS WITH A LOW NITROGEN CONTENT, THE VOLUME OF SULPHURIC ACID 0.1 N TO BE PLACED IN THE COLLECTING FLASK MAY BE REDUCED, IF NECESSARY, TO 10 OR 15 ML AND MADE UP TO 25 ML WITH WATER.
Slovenian: Pri proizvodih z nizko vsebnostjo dušika lahko količino žveplove kisline 0,1 N, ki jo moramo dodati v zbirno bučko, po potrebi zmanjšamo na 10 ali 15 ml in bučko do 25 ml dopolnimo z vodo.
English: IF THE FLASK OF THE DISTILLATION APPARATUS IS NOT FITTED WITH A DROPPING FUNNEL, ADD THE SODIUM HYDROXIDE IMMEDIATELY BEFORE CONNECTING THE FLASK TO THE CONDENSER, POURING THE LIQUID SLOWLY DOWN THE SIDES OF THE CONDENSER SO THAT IS DOES NOT MIX WITH THE ACID SOLUTION.
Slovenian: Če bučka naprave za destilacijo nima kapalnega lijaka, dodamo natrijev hidroksid, tik preden povežemo bučko s kondenzatorjem, tekočino pa počasi dolivamo po stenah kondenzatorja, da se ne zmeša s kislo raztopino.
English: HYDROCHLORIC ACID, D: 1.125.
Slovenian: Klorovodikova kislina, d: 1,125.
English: ABOUT 0.2 % (W/V) FRESHLY PREPARED SOLUTION OF PEPSIN IN HYDROCHLORIC ACID;
Slovenian: Približno 0,2 % (m/v) sveže pripravljene raztopine pepsina v klorovodikovi kislini;
English: ACTIVITY: 400 U/L.
Slovenian: aktivnost: 400 U/l.
English: WATER BATH OR INCUBATOR, SET AT 40* C MORE OR LESS 1* C.
Slovenian: Vodna kopel ali inkubator, nastavljen na 40 °C 1 °C.
English: ADD 450 ML OF PEPSIN HYDROCHLORIDE SOLUTION PREVIOUSLY HEATED TO 40* C AND SHAKE TO PREVENT THE FORMATION OF AGGLOMERATES.
Slovenian: Dodamo 450 ml raztopine pepsinhidroklorida, ki smo ga predtem segreli na 40 °C, in stresamo, da preprečimo sprijemanje.
English: PLACE THE FLASK IN THE WATER BATH OR INCUBATOR AND LEAVE THERE FOR 48 HOURS.
Slovenian: Bučko postavimo za 48 ur na vodno kopel ali v inkubator.
English: SHAKE AFTER 8, 24 AND 32 HOURS.
Slovenian: Po 8, 24 in 32 urah jo pretresemo.
English: AFTER 48 HOURS, ADD 15 ML OF HYDROCHLORIC ACID, COOL TO 20* C, MAKE UP TO VOLUME WITH WATER AND FILTER.
Slovenian: Po 48 urah dodamo 15 ml klorovodikove kisline, ohladimo na 20 °C, dopolnimo z vodo do oznake in filtriramo.
English: TAKE 250 ML OF THE FILTRATE AND PLACE IN THE FLASK OF THE DISTILLATION APPARATUS.
Slovenian: 250 ml filtrata prenesemo v bučko aparature za destilacijo.
English: IF ANY FOAM SHOULD FORM, ADD A FEW DROPS OF ANTI-FOAMING EMULSION.
Slovenian: Če nastane pena, dodamo nekaj kapljic emulzije proti penjenju.
English: WHEN THE SOLUTION BECOMES CLEAR AND COLOURLESS (OR LIGHT GREEN IF A COPPER-BASED CATALYST IS USED), CONTINUE TO BOIL FOR ANOTHER HOUR.
Slovenian: Ko se raztopina zbistri in postane brezbarvna (ali svetlo zelena, če smo uporabili katalizator, ki vsebuje baker), pustimo, da vre še eno uro.
English: 1 ML OF SULPHURIC ACID
Slovenian: 1 ml žveplove kisline
English: N CORRESPONDS TO 1.4 MG OF NITROGEN.
Slovenian: 0,1 N ustreza 1,4 mg dušika.
English: 0.4, IN ABSOLUTE VALUE, FOR CONTENTS OF LESS THAN 20 %;
Slovenian: 0,4, v absolutni vrednosti, za vsebnosti pod 20 %,
English: %, IN RELATE VALUE, FOR CONTENTS OF NOT LESS THAN 20 % AND NOT MORE THAN 40 %;
Slovenian: 2,0 %, v relativni vrednosti, za vsebnosti med 20 % in 40 %,
English: 0.8, IN ABSOLUTE VALUE, FOR CONTENTS OF MORE THAN 40 %.
Slovenian: 0,8, v absolutni vrednosti, za vsebnosti nad 40 %.
English: DEFINITION:
Slovenian: Opredelitev:
English: THE UNIT OF PEPSIN IS DEFINED AS BEING THE QUANTITY OF THAT ENZYME WHICH, UNDER THE CONDITIONS OF THE METHOD, LIBERATES PER MINUTE, A QUANTITY OF HYDROXYARYL GROUPS WHICH, WHEN STAINED WITH THE FOLIN-CIOCALTEU REAGENT, HAS AN OPTICAL DENSITY CORRESPONDING TO THAT OF ONE UMOLE OF TYROSINE STAINED IN THE SAME MANNER.
Slovenian: Enota pepsina (E) je opredeljena kot količina encima, ki v pogojih metode na minuto sprosti toliko hidroksiarilnih skupin, da ima njihovo obarvanje z reagentom po Folin-Ciocalteuju za posledico optično gostoto, ki ustreza optični gostoti enega -mola tirozina pod enakimi pogoji.
English: PLACE 100 G OF SODIUM TUNGSTATE (NA2WO4.2H2O), 25 G OF SODIUM MOLYBDATE (NA2MOO4.2H2O) AND 700 ML OF WATER IN A 2 LITRE ROUND-BOTTOMED FLASK FITTED WITH A STANDARD GROUND-GLASS JOINT.
Slovenian: V 2-litrsko bučko z okroglim dnom in s standardnim brusom prenesemo 100 g natrijevega volframata (Na2WO4 × 2H2O), 25 g natrijevega molibdata (Na2MoO4 × 2H2O) in 700 ml vode.
English: ADD 50 ML OF PHOSPHORIC ACID (D: 1.71) AND 100 ML OF CONCENTRATED HYDROCHLORIC ACID (D: 1.19), CONNECT A REFLUX CONDENSER TO THE FLASK, BRING TO THE BOIL AND KEEP THE SOLUTION GENTLY BOILING FOR 10 HOURS.
Slovenian: Dodamo 50 ml fosforjeve kisline (d: 1,71) in 100 ml koncentrirane klorovodikove kisline (d: 1.19), bučko povežemo s povratnim hladilnikom, segrejemo do vrenja in pustimo, da raztopina 10 ur rahlo vre.
English: LEAVE TO COOL, DETACH THE REFLUX CONDENSER, ADD 175 G OF LITHIUM SULPHATE (LI2SO4.2H2O), 50 ML OF WATER AND 1 ML OF BROMINE.
Slovenian: Ohladimo, odstranimo povratni hladilnik, dodamo 175 g litijevega sulfata (Li2SO4 × 2H2O), 50 ml vode in 1 ml broma.
English: LEAVE TO COOL, TRANSFER THE SOLUTION TO A 1 LITRE GRADUATED FLASK, MAKE UP TO VOLUME WITH WATER, HOMOGENIZE AND FILTER.
Slovenian: Ohladimo, raztopino prelijemo v litrsko merilno bučko, dopolnimo z vodo do oznake, premešamo in filtriramo.
English: BEFORE USE, DILUTE 1 VOLUME OF THE REAGENT WITH 2 VOLUMES OF WATER.
Slovenian: Pred uporabo razredčimo en volumen reagenta z dvema volumnoma vode.
English: HAEMOGLOBIN SOLUTION:
Slovenian: Raztopina hemoglobina:
English: WEIGH A QUANTITY OF HAEMOGLOBIN (APPROX. 2 G OF PROTEIN SUBSTRATUM DETERMINED ACCORDING TO ANSON) CORRESPONDING TO 354 MG OF NITROGEN AND PLACE IN A 200 ML FLASK FITTED WITH A STANDARD GROUND-GLASS JOINT.
Slovenian: Natehtamo količino hemoglobina (približno 2 g beljakovinskega substrata, določenega po Ansonu), ki ustreza 354 mg dušika, in ga prenesemo v 200-mililitrsko bučko s standardnim brusom.
English: ADD A FEW ML OF HYDROCHLORIC ACID, CONNECT THE FLASK TO THE VACUUM PUMP AND SHAKE UNTIL THE HAEMOGLOBIN HAS COMPLETELY DISSOLVED.
Slovenian: Dodamo nekoliko mililitrov klorovodikove kisline, bučko spojimo z vakuumsko črpalko in stresamo, dokler se hemoglobin popolnoma ne raztopi.
English: RELEASE THE VACUUM AND, WHILE SHAKING, ADD HYDROCHLORIC ACID TO MAKE UP TO 100 ML.
Slovenian: Vakuum odstranimo in med stresanjem dodajamo klorovodikovo kislino do 100 ml.
English: STANDARD TYROSINE SOLUTION:
Slovenian: Standardna raztopina tirozina:
English: DISSOLVE 181.2 MG OF TYROSINE IN THE HYDROCHLORIC ACID AND MAKE UP TO 1 LITRE WITH THE SAME ACID (STICK SOLUTION).
Slovenian: Raztopimo 181,2 mg tirozina v klorovodikovi kislini in z isto kislino dopolnimo do enega litra (osnovna raztopina).
English: TAKE 20.0 ML AND DILUTE TO 100 ML WITH HYDROCHLORIC ACID.
Slovenian: 20,0 ml razredčimo do 100 ml s klorovodikovo kislino.
English: CHRONOMETER,
Slovenian: Kronometer,
English: ACCURACY:
Slovenian: točnost:
English: DISSOLVE 150 MG OF PEPSIN IN 100 ML OF HYDROCHLORIC ACID.
Slovenian: Raztopimo 150 mg pepsina v 100 ml klorovodikove kisline.
English: PIPETTE 2 ML OF THE SOLUTION INTO A 50 ML GRADUATED FLASK AND MAKE UP TO VOLUME WITH HYDROCHLORIC ACID.
Slovenian: Odpipetiramo 2 ml raztopine v 50-mililitrsko merilno bučko in dopolnimo s klorovodikovo kislino do oznake.
English: THE PH, CHECKED WITH THE PH-METER, MUST BE 1.6 MORE OR LESS 0.1.
Slovenian: Vrednost pH, ki jo izmerimo s pH-metrom, mora biti 1,6 0,1.
English: IMMERSE THE FLASK IN THE WATER BATH.
Slovenian: Bučko potopimo v vodno kopel.