What is the best bike setup for Zwift's Three Little Sisters tour?

I recommend a lightweight bike with good climbing capabilities for this route, such as the Specialized Tarmac, Canyon Ultimate, or Trek Emonda. These bikes offer a good balance of speed and climbing ability, which can be helpful on the steep ascents of the Three Little Sisters tour. The route will first tackle the Hilly KOM, Titan's Grove KOM, and finally, finish on the Volcano KOM. In terms of wheels, a set of lightweight climbing wheels such as the DT Swiss disc or Zipp 858's could be a good option, as they offer reduced weight and improved aerodynamics.