Before revision:  The Gag is found to lead to resolution limited punctae on the lipid membranes of the GUV . The introduction of the Gag  to a GUV solution containing RNA led to the budding of minivesicles on the inside surface of the GUV.
After revision:  The Gag is found to lead to resolution limited punctae on the lipid membranes of the GUV . The introduction of the Gag and urea to a GUV solution containing RNA led to the budding of minivesicles on the inside surface of the GUV.