Before revision: The application of microscopy in biomedical research has come a long way since Antonie van Leeuwenhoek discovery of URLanisms through his hand-crafted microscope. Countless innovations have propelled imaging techniques and have positioned fluorescence microscopy as a cornerstone of modern biology and as a method of choice for connecting omics datasets to their biological and clinical correlates. Still, regardless of how convincing imaging results look, they do not always convey meaningful information about the conditions in which they were acquired, processed and analyzed to achieve the presented results. Adequate record-keeping and quality control are therefore essential to ensure experimental rigor and datafidelity, to allow experiments to be reproducibly repeatedand to promote the proper evaluation, interpretation, comparison and re-use of the results.
After revision: High-resolution digital microscopy provides powerful tools for probing the real-time dynamics of subcellular structures, and adequate record-keeping and quality control are therefore essential to ensure experimental rigor and datafidelity, to allow experiments to be reproducibly repeatedand to promote the proper evaluation, interpretation, comparison and re-use of the results.