Before revision:  To address this technical bottleneck, we have created a series of Gateway shuttle vectors  , which facilitate the assembly of artificial genes and their expression in the budding yeast Saccharomyces cerevisiae.
After revision:  To address this technical bottleneck, we have created a series of Gateway shuttle vectors and an integration vector , which facilitate the assembly of artificial genes and their expression in the budding yeast Saccharomyces cerevisiae.