Before revision: A Serum inspissation or Fractional sterilization is a process of heating an article on 3 successive days as follows: Day Temperature Time Purpose 1 85°C 60 minutes Drying of the medium and killing the organisms in their vegetative form Time in between overnight incubation Growth of vegetative forms from spores 2 75 to 80°C 20 minutes killing the organisms in their vegetative form Time in between overnight incubation Growth of vegetative forms from any spores remaining 3 75 to 85°C 20 minutes killing the organisms in their vegetative form Pathologic inspissation In cystic fibrosis, inspissation of secretions in the respiratory and gastrointestinal tracts is a major mechanism of causing the disease.
After revision: as well as the leftover sporesA Serum inspissation or Fractional sterilization is a process of heating an article on 3 successive days as follows: Day Temperature Time Purpose 1 85°C 60 minutes Drying of the medium and killing the organisms in their vegetative form Time in between overnight incubation Growth of vegetative forms from spores 2 75 to 80°C 20 minutes killing the organisms in their vegetative form Time in between overnight incubation Growth of vegetative forms from any spores remaining 3 75 to 85°C 20 minutes killing the organisms in their vegetative form Pathologic inspissation In cystic fibrosis, inspissation of secretions in the respiratory and gastrointestinal tracts is a major mechanism of causing the disease.