Before revision:  A Boltzmann machine for the accurate estimation of the field and coupling interactions, which is required for other studies in protein evolution and folding, is studied about learning methods , regularization models and a tuning method of regularization parameters in order to infer the interactions with reasonable characteristics . Using L_2 regularization for fields, group L_1 for couplings is shown to be very effective for parse couplings in comparison with L_2 and with L_1.
After revision:  A Boltzmann machine for the accurate estimation of the field and coupling interactions, which is required for other studies in protein evolution and folding, is studied about learning methods , regularization models and a tuning method of regularization parameters in order to infer the interactions with reasonable characteristics . Using L_2 regularization for fields, group L_1 for couplings is shown to be very effective for sparse couplings in comparison with L_2 and with L_1.