Before revision: For a sample with fluorophore height in the range of 10nm , oxide thickness around 50nm would be best because the FLIC intensity curve is steepest here and would produce the greatest contrast between fluorophore heights.
After revision: For a sample with fluorophore height in the range of 10 nm , oxide thickness around 50 nm would be best because the FLIC intensity curve is steepest here and would produce the greatest contrast between fluorophore heights.