Before revision:  To show this, we used ectopic integration to characterize cells that carry either the MRE11 or mre11A470T alleles at the genomic g site, and a second opposing mre11A470T or MRE11 allele, respectively, at an ectopic site. We find that segregation of MRE11 or mre11A470T telosomes is dependent primarily on its initial genotype, but not on any subsequent genotypic change supporting the conservative segregation of a replicating chromatid
After revision:  To show this, we used ectopic integration to characterize cells that carry either the MRE11 or mre11A470T alleles at the genomic g allele at an ectopic site in a wild-type strain lead to unexpectedly differing results. In each case, the replicated sister chromatids inherit telosomes containing only the protein encoded by the genomic mre11 locus, even in the presence of protein encoded by the opposing ectopic allele. We hypothesize that the telosome segregates by a conservative mechanism. These data support a mechanism for the linkage between sister chromatid replication and maintenance of either identical mutant or identical wild-type telosomes after replication of sister chromatids. These data suggest the presence of an active mechanism for chromatin segregation in yeast.