Before revision:  Our measurements based on using four independent techniques, static light scattering, dynamic light scattering (DLS), size-exclusion chromatography (SEC), and fluorescence correlation spectroscopy (FCS) , lead to this conclusion for the enzymes urease (emphasized in the main text ), and hexokinase and acetylcholinesterase (data shown in the supplementary material), each of which catalyzes different reactions. The conclusion is robust for urease obtained from different sources. For substrate concentrations in the range of biological relevance, meaning below kM , these enzymes do not dissociate and enhanced diffusion is observed nonetheless.
After revision:  Our measurements based on using four independent techniques, static light scattering, dynamic light scattering (DLS), size-exclusion chromatography (SEC), and fluorescence correlation spectroscopy (FCS) , lead to this conclusion for the enzymes urease (emphasized in the main text ), and hexokinase and acetylcholinesterase with data presented in supplementary information. For substrate concentration regimes below kM at which these enzymes do not dissociate and enhanced diffusion is observed nonetheless.