Before revision: Number of cells to be analyzed scRNA-Seq Generally speaking, for a typical bulk cellRNA-sequencing (RNA-seq) experiment,ten million reads are generated and a gene with higher than the threshold of 50 reads per kb per million reads (RPKM) is considered expressed.
After revision: sequenced andNumber of cells to be analyzed scRNA-Seq Generally speaking, for a typical bulk cellRNA-sequencing (RNA-seq) experiment,ten million reads are generated and a gene with higher than the threshold of 50 reads per kb per million reads (RPKM) is considered expressed.