Before revision:  To investigate whether a prion-based transmission "genetics" was incompatible with the evolution of Metazoa , we identify genes conserved in fungi and choanoflagellates but lost in animals. We show that both eukaryotic clpB amyloid disaggregases , HSP104 and its nuclear-encoded mitochondrial endo-ortholog HSP78, were lost in the stem-metazoan lineage along with only a small number of other relevant genes.
After revision:  To investigate whether a prion-based transmission "genetics" was incompatible with the evolution of Metazoa , we identify conserved eukaryotic genes that were lost in stem-metazoans. Among the few genes lost are both eukaryotic clpB amyloid disaggregases , HSP104 and its nuclear-encoded mitochondrial endo-ortholog HSP78, were lost in the stem-metazoan lineage along with only a small number of other relevant genes.