Before revision: Multiplexed immunofluorescence tissue imagingenables precise spatial assessment of protein expression in medical resection specimens. However, tissue sections are stained with a mixture of antibodies , DNA and RNA markers, the detection of weak or broken edges due to fluorescent membrane staining artifacts between touching or overlapping cells is a long term studied problem, and is an active research topic in biomedical image analysis.
After revision: Multiplexed immuno-fluorescence tissue imaging, allowing simultaneous detection of molecular properties of cells, is an essential tool for characterizing the complex cellular mechanisms in translational research and clinical practice. New image analysis approaches are needed because tissue section stained with a mixture of antibodies , DNA and RNA markers, the detection of weak or broken edges due to fluorescent membrane staining artifacts between touching or overlapping cells is a long term studied problem, and is an active research topic in biomedical image analysis.