Before revision: Linked-read sequencing technology allowed researchers to identify more than 3000 differentially methylated regions between these twins discordant for Alzheimer’s Disease, and investigation of these differentially methylated regions eventually led to identification of genes enriched in neurodevelopmental processes, neuronal signalling, and immune system functions Advantages Limitations Advantages Wide range of genomic applications and scientific questions, including de novo genome assembly, haplotype phasing, structural variant analysis, and transcriptome and epigenetic analysis.
After revision: Linked-read sequencing technology allowed researchers to identify more than 3000 differentially methylated regions between these twins discordant for Alzheimer’s Disease, and investigation of these differentially methylated regions eventually led to identification of genes enriched in neurodevelopmental processes, neuronal signalling, and immune system functions Advantages Use Wide range of genomic applications and scientific questions, including de novo genome assembly, haplotype phasing, structural variant analysis, and transcriptome and epigenetic analysis.