Before revision:  The OCT4 fluctuations in five minute intervals follow a Laplace distribution, with BMP4 addition provoking smaller changes and tighter self-regulation, particularly in the differentiated fate group. This quantitative framework provides a basis for comparison to other experiments, and the development of mathematical models of pluripotency .
After revision:  The OCT4 fluctuations in five minute intervals follow a Laplace distribution, with BMP4 treatment. Our framework allows us to quantify the relevant properties of proliferating hESC colonies and the procedure is widely applicable to other transcription factors and cell populations .