Before revision: High-resolution digital microscopy provides powerful tools for probing the real-time dynamics of subcellular structures, and adequate record-keeping is necessary to evaluate results, share data, and allow experiments to be repeated.
After revision: The application of microscopy in biomedical research has come a long way since Antonie van Leeuwenhoek discovered URLanisms. Countless innovations have positioned light microscopy as a cornerstone of modern biology and a method of choice for connecting omics datasets to their biological and clinical correlates. Still, regardless of how convincing published imaging data looks, it does not always convey meaningful information about the conditions in which it was acquired, processed, and analyzed. Adequate record-keeping is necessary to evaluate results, share data, and allow experiments to be repeated.