Before revision:  The capability of exploring ETp by CP-AFM  over a significant range of temperatures, with varying tip force to detect possible pressure-induced changes in the sample , significantly adds to the ability to study ETp through proteins and of using ETp to study proteins, with this approach .
After revision:  The capability of exploring ETp by CP-AFM over a significant range of temperatures, with varying tip force to detect possible pressure-induced changes in the sample , is that for ETp measurements on floppy systems, such as proteins, the stress applied to the sample should be kept constant or, at least controlled during measurement .