Before revision:  Yet, despite advances in high-throughput sequencing technologies and bioinformatics algorithms, metagenomics still has limitations barring mass clinical acceptance and broad implementation. Algorithms currently struggle to balance sensitivity and specificity of discovered biomarkers, genomic reference databases are incomplete, and studies are frequently not reproducible beyond the original environmental settings.
After revision:  Yet, despite advances in high-throughput sequencing technologies and bioinformatics algorithms, metagenomics still has limitations barring the tools from clinical validation.