Before revision: Reconstituted nicotinic acetylcholine receptors ( nAChR ) exhibit significant gain-of-function upon addition of cholesterol to reconstitution mixtures, and experimentally observed clustering of nAChRs within membranes is cholesterol-sensitive, leading to a common expectation that nAChRs will partition into cholesterol-rich liquid ordered ("raft" ) domains .
After revision: Reconstituted nicotinic acetylcholine receptors ( nAChR ) exhibit significant gain-of-function upon addition of cholesterol to reconstitution mixtures, and experimentally observed clustering of nAChRs within membranes is cholesterol-sensitive, leading to a common expectation that nAChRs will partition into cholesterol-rich liquid ordered ("raft" or l_o) domains or cholesterol-poor liquid-disordered (l_{do .