Before revision: Deciphering the workings of a brain (and its alterations due to pathologies and disorders) requires real-time mapping of the activity of all neurons. Recently, this formidable task has been brought into reach by the implementation of light sheet fluorescence (LSF) microscopy on the transparent zebrafish larva, a simple yet representative model of the vertebrate brain. This application is however hindered by the use of visible light for fluorescence excitation , inducing unwanted visual stimulation that can only be partially overcome resorting to complex excitation geometries.
After revision: Deciphering the workings of a brain (and its alterations due to pathologies and disorders) requires real-time whole-brain functional imaging in zebrafish larvae. Conventional one photon LSFM can however induce undesirable visual stimulation due to the use of visible light for fluorescence excitation , inducing unwanted visual stimulation that can only be partially overcome resorting to complex excitation geometries.