Before revision:  We show that the cell's electrical opacitywhich is a metric that reveals the properties of the cell membrane is strongly influenced by the physical design parameters of the cytometer and should therefore be carefully defined and interpreted in experiments .
After revision:  We show that the cell's electrical opacitywhich is a metric that reveals the properties of the cell impedance at high to low frequency) which is commonly used to distinguish between sub-population of the target cells( e.g. lymphocytes vs monocytes when counting white blood cells).Moreover, we highlight that the opacity response vs frequency can significantly vary depending upon whether the absolute cell impedance or the differential output impedance is used in the calculation .