Before revision:  A third validation was performed using the matched CG sample of the transgender women ( TW) application sample . Subsequently, the classifier was applied to N = 25 TW . Finally, we compared brain volumes of CG-men, women and TW pre /post treatment ( CHT ) in a univariate analysis controlling for sexual orientation, age and total brain volume.
After revision:  A third validation was performed using the matched CG sample of the transgender women ( TW) application sample . Subsequently, the classifier was applied to N = 25 TW . Finally, we compared brain volumes of CG-men, women and TW pre /post treatment ( cross-sex hormone treatment ) in a univariate analysis controlling for sexual orientation, age and total brain volume.