Before revision:  Here, we show that an individual reaction cycle can be regarded as a kind of Szilard engine. Accordingly, the work done at the individual cycle level can be calculated by measuring the amount of information transmitted. As a result, we can obtain a method for quantifying the information transduction of biochemical reaction cascades .
After revision:  Here, we aimed to develop a method for quantifying this signal transduction. We showed that the channel capacities of individual steps in a given general model cascade were equivalent in an independent manner, and were given by the entropy production rate. Signal transduction was transmitted by fluctuation of the entropy production rate and quantified transduction was estimated by the work done in individual steps. If the individual step representing the modification to demodification of the signal molecules is considered to be a Szilard engine, the maximal work done is equivalent to the chemical potential change of the messenger that is consumed during the modification reaction. Our method was applicable to calculate the channel capacity of the MAPK cascade. In conclusion, our method is suitable for quantitative analyses of signal transduction .