Before revision: Ingredients Mammillaria miegiana on liquid MS media The original ingredients set out by Mirashige and Skoog in 1962 are as follows: Inorganic salts Ammonium nitrate (NH4NO3) 400 mg/l Potassium chloride (KCl) 65 mg/l Potassium nitrate (KNO3) 80 mg/l .
After revision: Ingredients Mammillaria miegiana on liquid MS media The original ingredients set out by Mirashige and Skoog in 1962 are as follows: Major salts (macronutrients) per litre Ammonium nitrate (NH4NO3) 1650 mg/l Calcium chloride (CaCl2 · 2H2O) 440 mg/l Magnesium sulfate (MgSO4 · 7H2O) 370 mg/l