Before revision:  The changes in CP-AFM current-voltage ( I-V) curves for holo-Az and apo-Az, measured between 250 - 370K, are strikingly different. While ETp across holo-Az at low force (6 nN) is temperature-independent over the whole examined range, ETp across apo-Az is thermally activated , with calculated activation energy of 600 meV.
After revision:  The changes in CP-AFM current-voltage ( I-V) curves for holo-Az and apo-Az, measured between 250 - 373K) and of applied tip force (6-12 nN). By varying both temperature and force in CP-AFM, we find that the ETp mechanism can alter with a change in the force applied via the tip to the proteins. As the applied force increases, ETp via Az changes from temperature-independent over the whole examined range, ETp across apo-Az is thermally activated , with calculated activation energy of 600 meV.