Before revision: Initially the only chemical treatment used by researchers was treatment with Potassium hydroxide (KOH) to remove humic substances; defloculation was accomplished through surface treatment or ultra-sonic treatment, although sonification may cause the pollen exine to rupture.
After revision: Initially the only chemical treatment used by researchers was treatment with potassium hydroxide (KOH) to remove humic substances; defloculation was accomplished through surface treatment or ultra-sonic treatment, although sonification may cause the pollen exine to rupture.