Before revision: We demonstrate that the oft-reported enhanced diffusion of certain enzymes at substrate concentrations above biological concentrations, meaning above kM(the Michaelis-Menten constant ), can be accounted for by dissociation of these oligomericenzymes into their smaller subunits.
After revision: The concept that catalytic enzymes can act as molecular machines transducing chemical activity into motion has conceptual and experimental support, but much of the claimed support comes from experimental conditions where the substrate concentration is higher than biologically relevant and accordingly exceeds kM, the oft-reported enhanced diffusion of certain enzymes at substrate concentrations above biological concentrations, meaning above kM(the Michaelis-Menten constant ), can be accounted for by dissociation of these oligomericenzymes into their smaller subunits.