Before revision:  We present evolutionary couplings for 160 RNAs of unknown structure (available at URL and use these predictions to shed light on the mechanism of tRNA sensing by the T-box riboswitch, as well as the evolutionary history of the ribozyme RNase P. We anticipate that this approach will shed light on the structure and function of as yet less known RNAgenes .
After revision:  We present evolutionary couplings for 160 non-coding RNA families. Beyond 3D structure prediction, evolutionary couplings help identify important functional interactions, e.g., at switch points in riboswitches and at a complex nucleation site in HIV. Aided by accelerating sequence accumulation, evolutionary coupling analysis can accelerate the discovery of functional interactions and 3D structures involving RNA .