Before revision:  We present CLEAR, a workflow that identifies robust transcripts in lcRNA-seq data for between-group comparisons. To develop CLEAR, we compared DEGs from RNA extracted from FACS-derived CD5+ and CD5- cells from a single chronic lymphocytic leukemia patient diluted to input RNA levels of 10-, 100- and 1,000pg.
After revision:  We present CLEAR, a workflow that identifies robust transcripts in lcRNA-seq data for differentially expressed gene (DEG) analysis. Libraries at three input amounts of FACS-derived CD5+ and CD5- cells from a single chronic lymphocytic leukemia patient diluted to input RNA levels of 10-, 100- and 1,000pg.