Before revision: Molecular motors are nonequilibrium open systems that convert chemical energy to mechanical work. Here we investigate the nonequilibrium energetics of a single molecule kinesin by measuring the motion of an attached probe particle and its response to external forces with optical tweezers.
After revision: Nonequilibrium energetics of single molecule translational motor kinesin was investigated by measuring heat dissipation from the violation of the motion of an attached probe particle and its response to external forces with optical tweezers.