Before revision:  Here we describe an algorithm that allows for individual < 1 MDa particle images to be aligned without frame averaging when imaged with 2.5 e^{- . The algorithm maximizes the overall correlation of the shifted frames with the sum of the shifted frames.
After revision:  Here we describe an algorithm that allows for individual < 1 MDa particle images to be aligned without frame averaging or linear trajectories . The algorithm maximizes the overall correlation of the shifted frames with the sum of the shifted frames.