Before revision:  We term this approach limiting-cell RNA-seq (lcRNA-seq) . Unlike scRNA-seq, which focuses on 'cell-atlasing', lcRNA-seq focuses on identifying differentially expressed genes (DEGs) between experimental groups. This requires accounting for systems noise which can obscure biological differences. We present CLEAR, a workflow that identifies robust transcripts in lcRNA-seq data for between-group comparisons.
After revision:  We term this approach limiting-cell RNA-seq (lcRNA-seq) data for downstream analyses. Herein, we present CLEAR, a workflow that identifies robust transcripts in lcRNA-seq data for between-group comparisons.