Before revision:  We show that the cell's electrical opacitywhich is a metric that reveals the properties of the cell membrane is strongly influenced by the physical design parameters of the cytometer and should therefore be carefully defined and interpreted in experiments .
After revision:  In particular, the area of microelectrodes can have significant effect on cells electrical opacity( the ratio of the cell membrane is strongly influenced by the physical design parameters of the cytometer and should therefore be carefully defined and interpreted in experiments .