Before revision:  In fact, the majority of the eukaryotic genome does not code for proteins, and thousands of conserved RNAs of currently unknown function have been identified . Knowledge of 3D structure could can help elucidate the function of these RNAs but despite outstanding word using X-ray crystallography, NMR and cryoEM, structure determination remains low-throughput. RNA structure prediction in silico is a promising alternative. However, 3D structure prediction for large RNAs requires tertiary contacts between distant secondary structural elements that are difficult to infer with existing methods.
After revision:  In fact, the majority of the eukaryotic genome does not code for proteins, and thousands of conserved RNAs of currently unknown function have been identified . Knowledge of 3D structure could can help elucidate the function , but in silico 3D structure prediction for large RNAs requires tertiary contacts between distant secondary structural elements that are difficult to infer with existing methods.