Before revision: Chemicals that form DNA adducts Figure 3: DNA damaged by carcinogenic 2-aminofluorene acetaldehyde, a significant constituent of tobacco smoke cisplatin, which binds to DNA and causes crosslinking (leading to cell death) DMBA (7,12-dimethylbenz(a)anthracene) malondialdehyde, a naturally-occurring product of lipid peroxidation polycyclic aromatic hydrocarbons (PAHs) nitro-PAHs Nitrosamines Aflatoxins Mustards aromatic amines heterocyclic aromatic amines URL methylating agents other alkylating agents Haloalkanes Testing methods 32P-postlabeling assay: 32P-postlabeling assays screen for DNA adducts by transferring 32P-ATP into a carcinogenic labeled nucleotide sequence, with selectivity favoring modified nucleotides.
After revision: Chemicals that form DNA adducts Figure 3: DNA damaged by carcinogenic 2-aminofluorene acetaldehyde, a significant constituent of tobacco smoke cisplatin, which binds to DNA and causes crosslinking (leading to cell death) DMBA (7,12-dimethylbenz(a)anthracene) malondialdehyde, a naturally-occurring product of lipid peroxidation polycyclic aromatic hydrocarbons (PAHs) nitro-PAHs Nitrosamines Aflatoxins Mustards aromatic amines heterocyclic aromatic amines URL methylating agents other alkylating agents Haloalkanes Detection methods 32P-postlabeling assay: 32P-postlabeling assays screen for DNA adducts by transferring 32P-ATP into a carcinogenic labeled nucleotide sequence, with selectivity favoring modified nucleotides.