Before revision:  This is combined with the interface-scale prediction using statistical learning. The learning algorithm uses - for training - a fraction of the mutagenesis data, with the remainder of the data being used for validation .
After revision:  Our study goes all the way from resource-light, multiscale computational predictions of crucial hotspot inter-atomic interactions to experimental validation by biophysical assays of the effect of site-directed mutagenesis on capsid assembly .