Before revision:  Metabolic networks are often studied with optimization methods applied to constraint-based steady state models. But, a corresponding dynamic modeling framework including a tight interplay between metabolic fluxes and gene expression is currently lacking. Due to that, the cost of producing enzymes so far could not be taken into account in dynamic optimization of metabolic fluxes. Here , we present a modeling framework combining the metabolic network and the enzyme production costs.
After revision:  Metabolic networks are often studied at steady state using constraint-based models and optimization techniques. However, metabolic adaptations driven by changes in gene expression cannot be analyzed by steady state models, as these do not account for temporal changes in biomass composition. Here we present a modeling framework combining the metabolic network and the enzyme production costs.