Before revision: Digests 1: EcoRI 2: HindIII 3: EcoRI + HindIII (optional and not discussed) Resultant Fragments : approximate sizes 1: 3 kb, 5 kb 2: 2 kb, 6 kb 3: 2 kb, 1 kb, 5 kb, Hypothetical Multiple Cloning Site of Vector 5'-----HindIII-EcoRI----3' Discussion The EcoRI digest excises the insert yielding fragments of 3 kb and 5 kb.
After revision: Digests 1: EcoRI 2: HindIII 3: EcoRI + HindIII (optional and not discussed) Resultant fragments : approximate sizes 1: 3 kb, 5 kb 2: 2 kb, 6 kb 3: 2 kb, 1 kb, 5 kb, Hypothetical multiple cloning site of vector 5'-----HindIII-EcoRI----3' Discussion The EcoRI digest excises the insert yielding fragments of 3 kb and 5 kb.