Before revision: Fabrication Some considerations for microfluidic devices relating to cell culture include: fabrication material (e.g., polydimethylsiloxane (PDMS), polystyrene) culture region geometry control system for delivering and removing media when needed using either passive methods (e.g., gravity-driven flow, capillary pumps, or Laplace pressure based ‘passive pumping’ ) or a flow-rate controlled device (i.e., perfusion system) Fabrication material is crucial as not all polymers are biocompatible, with some materials such as PDMS causing undesirable adsorption or absorption of small molecules.
After revision: Fabrication Some considerations for microfluidic devices relating to cell culture include: fabrication material (e.g., polydimethylsiloxane (PDMS), polystyrene) culture region geometry control system for delivering and removing media when needed using either passive methods (e.g., gravity-driven flow, capillary pumps, or Laplace pressure based 'passive pumping' ) or a flow-rate controlled device (i.e., perfusion system) Fabrication material is crucial as not all polymers are biocompatible, with some materials such as PDMS causing undesirable adsorption or absorption of small molecules.