Before revision:  Here we describe a custom-made 2P LSF microscope that overcomes this limit, being specifically designed to perform whole-brain cellular-resolution calcium imaging in zebrafish larvae quintuplicating previous volumetric acquisition frequency (5 Hz) . ...
After revision:  Here we describe a custom-made 2P LSFM setup designed for non-invasive imaging that enables quintuplicating state-of-the-art volumetric acquisition rate of the larval zebrafish brain (5 Hz) . ...