Before revision: The principle of pyrosequencing was first described in 1993Mostafa Ronagi, Nyren, Petersson and Uhlen (1993) “Solid Phase DNA Minisequencing by an Enzymatic Luminometric Inorganic Pyrophosphate Detection Assay” Analytical Biochemistry 208 (1), 171-175, URL by Bertil Pettersson, Mathias Uhlen and Pål Nyren by combining the solid phase sequencing methodUhlen (1989) ”Magnetic separation of DNA” Nature 340: 733-4, URL using streptavidin coated magnetic beads with recombinant DNA polymerase lacking 3´to 5´exonuclease activity (proof-reading) and luminescence detection using the firefly luciferase enzyme.Nyren and Lundin (1985) “Enzymatic method for continuous monitoring of inorganic pyrophosphate synthesis” Analytiocal Biochemistry 151 (2): 504-509.
After revision: ,The principle of pyrosequencing was first described in 1993Mostafa Ronagi, Nyren, Petersson and Uhlen (1993) “Solid Phase DNA Minisequencing by an Enzymatic Luminometric Inorganic Pyrophosphate Detection Assay” Analytical Biochemistry 208 (1), 171-175, URL by Bertil Pettersson, Mathias Uhlen and Pål Nyren by combining the solid phase sequencing methodUhlen (1989) ”Magnetic separation of DNA” Nature 340: 733-4, URL using streptavidin coated magnetic beads with recombinant DNA polymerase lacking 3´to 5´exonuclease activity (proof-reading) and luminescence detection using the firefly luciferase enzyme.Nyren and Lundin (1985) “Enzymatic method for continuous monitoring of inorganic pyrophosphate synthesis” Analytiocal Biochemistry 151 (2): 504-509.