Before revision:  An experiment that an unbiased estimator achieving the Fisher information and Cramer-Rao lower bound (CRLB) localizes emitters frame by frame independently is simulated and the result demonstrates the superior sensitivity of RMSMD over the other four metrics . The RMSMD of an information-achieving nanoscopy image benchmarks localization nanoscopy images and localization algorithms .
After revision:  An experiment that an unbiased estimator achieving the Fisher information and Cramer-Rao lower bound (CRLB) localizes emitters frame by frame independently is simulated and the result demonstrates the superior sensitivity of RMSMD over the other four metrics .