Before revision:  We evaluate the internal self-regulation of OCT4  , quantify the intra-cellular fluctuations and consider the diffusive nature of OCT4 over time for individual  and pairs of related cells. This quantitative framework provides a basis for comparison to other experiments, and the development of mathematical models of pluripotency.
After revision:  We evaluate the internal self-regulation of OCT4 , quantify the intra-cellular fluctuations and consider the diffusive nature of OCT4 over time for individual and pairs of their descendants. After possible asymmetric splitting, OCT4 abundance fluctuates sub-diffusively, showing anti-persistent self-regulation with a Hurst exponent of 0.38. Auto-correlation analysis shows anti-persistence for five hours or longer is seen in 86