Before revision: The principle of pyrosequencing was first described in 1993Nyren, Petersson and Uhlen (1993) “Solid Phase DNA Minisequencing by an Enzymatic Luminometric Inorganic Pyrophosphate Detection Assay” Analytical Biochemistry 208 (1), 171-175, URL by, Bertil Pettersson, Mathias Uhlen and Pål Nyren by combining the solid phase sequencing methodUhlen (1989) ”Magnetic separation of DNA” Nature 340: 733-4, URL using streptavidin coated magnetic beads with recombinant DNA polymerase lacking 3´to 5´exonuclease activity (proof-reading) and luminescence detection using the firefly luciferase enzyme.Nyren and Lundin (1985) “Enzymatic method for continuous monitoring of inorganic pyrophosphate synthesis” Analytiocal Biochemistry 151 (2): 504-509.
After revision: The principle of pyrosequencing was first described in 1993Nyren, Pettersson and Uhlen (1993) “Solid Phase DNA Minisequencing by an Enzymatic Luminometric Inorganic Pyrophosphate Detection Assay” Analytical Biochemistry 208 (1), 171-175, URL by, Bertil Pettersson, Mathias Uhlen and Pål Nyren by combining the solid phase sequencing methodUhlen (1989) ”Magnetic separation of DNA” Nature 340: 733-4, URL using streptavidin coated magnetic beads with recombinant DNA polymerase lacking 3´to 5´exonuclease activity (proof-reading) and luminescence detection using the firefly luciferase enzyme.Nyren and Lundin (1985) “Enzymatic method for continuous monitoring of inorganic pyrophosphate synthesis” Analytiocal Biochemistry 151 (2): 504-509.