Before revision:  This suggested that the formation of curved interfaces , necessary for the creation of a lipid droplet, were driven or supported by the process of lipid de-mixing. In the case of Saccharomyces cerevisiae LDs eventually detached from the ER at a critical diameter of about 30-50 nm.
After revision:  This suggested that the formation of curved interfaces was supported by or even driven by lipid demixing. In addition, we show that demixing is thermodynamically necessary as LD cannot bud-off otherwise. In the case of Saccharomyces cerevisiae LDs eventually detached from the ER at a critical diameter of about 30-50 nm.