Before revision:  Here, we combine these two biophysical methodologies in a single home-made instrument to enable the simultaneous detection of orthogonal fluorescence polarization signals from single fluorescent protein molecules used as common reporters on the localization on biomolecules in cellular processes , whose spatial location can be pinpointed to a super-resolved precision better than the diffraction-limited optical resolution  .
After revision:  Here, we combine these two biophysical methodologies in a single home-made instrument to enable the simultaneous detection of orthogonal fluorescence polarization signals from single fluorescent protein molecules used as common reporters on the localization on biomolecules in cellular processes , whose spatial location can be pinpointed to a super-resolved precision better than the diffraction-limited optical resolution , as well as estimation of molecular stoichiometry based on the brightness of individual fluorophores .