Before revision:  In this study, we develop an in vitrovascularized tumor platform that allows for investigation of tumor-stromal interactions in three breast tumor derived cell lines of varying aggressiveness: MDA-IBC3, SUM149, and  MDA-MB-231 . The platform recreates key features of breast tumors, including increased vascular permeability, vessel sprouting, and ECM remodeling. Morphological and quantitative analysis reveals differential effects from each tumor cell type on endothelial coverage, permeability, expression of VEGF, and collagen remodeling. Triple negative tumors, SUM149 and MDA-MB-321, resulted in a significantly (p<0.05) higher endothelial permeability and decreased endothelial coverage  compared to the control TIME only platform.
After revision:  In this study, we develop an in vitrovascularized tumor platform that allows for investigation of tumor-stromal interactions in three breast tumor derived cell lines of varying aggressiveness: MDA-IBC3, SUM149, and MDA-MB-231 (triple negative). An acellular collagen platform with an endothelial blood vessel served as control. SUM149 and MDA-MB-321, resulted in a significantly (p<0.05) higher endothelial permeability and decreased endothelial coverage compared to the control TIME only platform.