Before revision:  In regenerative medicine, one approach to generate 3D tissues has been to seed a porous scaffold with cells and growth factors under pulsatile flow conditions.
After revision:  In particular, the homeostasis of blood vessels is dependent on shear stress. To mimic this process ex vivo, efforts have been made to seed scaffolds with vascular and other cell types in the presence of growth factors and under pulsatile flow conditions.