Before revision:  our simulations confirm one of them is critical for viral binding . Also, since mutations in polar residues in another putative network do not change viral binding, we can conclude the second network is likely an artifact of crystallization. Finally, we found a viral site known to be critical for infection , may mark an important evolutionary suppressor site for infection-diminishing hTfR1 mutants.
After revision:  our simulations confirm one of them is critical for the binding interaction. Third, one viral site known to be critical for infection , may mark an important evolutionary suppressor site for infection-diminishing hTfR1 mutants.