Before revision:  In the case of Saccharomyces cerevisiae LDs eventually detached from the ER at a critical diameter of about 30-50 nm. We concluded that if the standard model of LD formation is valid, LD biogenesis is a two step process.
After revision:  In the case of Saccharomyces cerevisiae LDs eventually detached from the ER at a critical diameter of about 13 nm. This diameter is far below the experimentally determined size of typical yeast LD. Thus, we concluded that if the standard model of LD formation is valid, LD biogenesis is a two step process.