Before revision:  A universal and objective metric , which is crucial and necessary to evaluate qualities of nanoscopy images and performances of localization algorithms , has not yet been established. The metrics of root mean square error (RMSE or accuracy) , precision, recall, and Jaccard index (JAC) are currently used in the literature but in certain conditions fail in distinguishing qualities of different nanoscopy images .
After revision:  A universal and objective metric , which is crucial and necessary to evaluate qualities of nanoscopy images and performances of localization algorithms . In this paper, we propose root mean square error (RMSE or accuracy) , precision, recall, and Jaccard index (JAC) are currently used in the literature but in certain conditions fail in distinguishing qualities of different nanoscopy images .