Before revision: "As I remember, the “binary” refers to the function of interest being divided into two parts encoded by two separate plasmids rather than two bacterial hosts: we used the term “shuttle vectors” to refer to the multiple host property."
After revision: "As I remember, the "binary" refers to the function of interest being divided into two parts encoded by two separate plasmids rather than two bacterial hosts: we used the term "shuttle vectors" to refer to the multiple host property."