Before revision: Direct cDNA preamplification protocols developed for single-cell RNA-seq (scRNA-seq) have enabled transcriptome profiling of  rare cells without having to pool multiple samples or to perform RNA extraction.
After revision: Direct cDNA preamplification protocols developed for single-cell RNA-seq (scRNA-seq) have enabled transcriptome profiling of precious clinical samples and rare cells without having to pool multiple samples or to perform RNA extraction.