Before revision: A central challenge in industrial microbiological applications is engineering of genetic networks to achieve a target yield or steady state concentration. This is a particularly challenging problem when optimizing under novel reaction conditions where canonical models of metabolic pathways are no longer valid. In these scenarios, the biological systems are entirely represented by data for which no direct methods exist to optimize the reaction output. We introduce a datadriven model discovery approach that leverages Koopman operator theory and time-series expression measurements to discover models that predict untested reaction outcomes. These Koopman models allow us to design control strategies to achieve target steady state concentrations for products of interest. We develop a model of the trypotophan pathway and illustrate this steady stateprogramming framework on this pathway. Tryptophan is an essential amino acid and is a valuable product in industrial microbiology.
After revision: This paper describes the optimal selection of a valuable product in industrial microbiology.