Before revision:  The observed ordering energetics suggests the probability of an intermediate collapsed-bubble conformation releasing energy, sufficient to unbind the bubble ends. An alternate energy pathway thus surfaces complementing molecular zipper and bubble state cooperativity transitions. Fluctuation theorem supports our argument, which we further validate from DNA solutions across varying oligomer lengths, base pair moieties, and concentrations. This new information of fluctuation driven ordering energetics can contribute to new insights on DNA transcription, the integral basis of life. The nanomechanical dynamic dissipation paradigm of investigating picoliter bio-samples as introduced offers a new method for investigating other interesting challenges such as folding of proteins and cell proliferation, which also evolve through similar energetics of non-equilibrium processes .
After revision:  The observed ordering energetics suggests the probability of an intermediate collapsed-bubble conformation releasing energy, sufficient to unbind bubble ends, assisting further unbinding. Fluctuation theorem explains the observations opening further avenues to investigate fluctuation kinetics in other biological phenomena that also proceed through similar NE energetics .