Before revision:  HER2+ MDA-IBC3 tumor cells expressed high levels of VEGF (p<0.01) and induced vessel sprouting. Vessels sprouting was tracked for 3 weeks and with increasing time exhibited formation of multiple vessel sprouts that invaded into the ECM and surrounded clusters of MDA-IBC3 cells . Both IBC cell lines, SUM149 and MDA-IBC3, resulted in a collagen ECM with significantly greater porosity with 1.6 and 1.1 fold higher compared to control, p<0.01.
After revision:  HER2+ MDA-IBC3 tumor cells expressed high levels of VEGF (p<0.01) platforms. Additionally, unique to the MDA-IBC3 platform, we observed progressive sprouting of the endothelium over time resulting in viable vessels with lumen. The newly sprouted vessels encircled clusters of MDA-IBC3 cells . Both IBC cell lines, SUM149 and MDA-IBC3, resulted in a collagen ECM with significantly greater porosity with 1.6 and 1.1 fold higher compared to control, p<0.01.