Before revision:  The fluctuations apparently favor entropy ordering over disordering at biologically relevant T< 42C in a non-equilibrium transition pathway. The observed ordering energetics suggests the probability of an intermediate collapsed-bubble conformation releasing energy, sufficient to unbind the bubble ends.
After revision:  The fluctuations apparently favor entropy ordering over disordering at biologically relevant T< 42C . This signifies that unravelling possibly proceeds via intermediate collapsed-bubble conformation releasing energy, sufficient to unbind the bubble ends.