Before revision:  For instance, the robustness of different designs against factors, such as the internal topology, or the influence of the staple pattern, are handled empirically. We have built a model for the folding and melting processes of DNA origamis that is able to reproduce accurately several thermodynamic quantities measurable from UV absorption experiments. The model can also be used to design a new distribution of crossovers that increases the robustness of the DNA template. The model provides predictions among which a few of them have been already successfully verified. Therefore, in spite of its complexity we propose an algorithm that gives the capability to design and fabricate templates with dedicated properties, a necessary step for technological development .
After revision:  For instance, the robustness of different designs against factors, such as the hysteresis between melting and annealing, as well as the dependence upon the topology of the scaffold. We show that cooperativity between staples is critical to quantitatively explain the folding process of DNA origamis .