Before revision: The 3’ of each gene is purposely designed to contain HaeIII R/M sequences, and when HaeIII methyltransferase was expressed from a M.HaeIII gene, it would methylate HaeIII R/M sequence and cause the gene to be resistant to restriction enzyme digestion.
After revision: The 3’ of each gene was purposely designed to contain HaeIII R/M sequences, and when HaeIII methyltransferase was expressed from a M.HaeIII gene, it would methylate HaeIII R/M sequence and cause the gene to be resistant to restriction enzyme digestion.