(B) B cells were purified from Pacs1+/+ and Pacs1-/- spleens and OCR was measured using a Seahorse metabolic flux analyzer. OCR after rotenone/Antimycin A treatment was subtracted from baseline OCR to give basal mitochondrial respiratory rate. Data shown are the mean of 7-10 technical replicates from three independent experiments. Error bars indicate mean ± SD. Two-tailed unpaired t test, *P < 0.05. 