Location of known Parkin ubiquitination sites within the Miro structure. MiroS is shown in a 180°‐rotated top view (relative to Fig 2). Four residues in dMiro correspond in sequence and structure to residues that are ubiquitinated by Parkin in human Miro1 (hMiro1) or human Miro2 (hMiro2) [22]. These are shown as spheres (K220, R240, K439 and K600; labelled with dMiro numbering). An alignment of a 10‐aa segment of dMiro, hMiro1 and hMiro2 flanking each ubiquitinated site is shown. The large blue 'K' within each sequence alignment indicates the ubiquitinated residue in hMiro1 or hMiro2, and a smaller, underlined K indicates that dMiro has a lysine in the same position. Residue S324, a candidate Pink1 phosphorylation site, is also shown in spheres. Note the side‐chain of K439 is disordered in the MiroS structure, so it is modelled as an alanine. dMiro/Dm Miro, Drosophila Miro; Hs Miro 1/2, human Miro1/2. 