C. Condensin binding assessed by chromosome spreading. Cut3-HAimmunofluorescence microscopy signals were quantified on n> 100 chromosome spreads exhibiting Dam1-GFP at kinetochores (mitotic, M) or lacking Dam1-GFP (interphase, I). For each strain, four representative nuclei extracted from a same image acquired with same settings are shown. Asterisks indicate nuclei corresponding to Cut3-HA surface plots. Bar: 5 µm. Boxes indicate the 25th, median and 75th percentile and whiskers the min and max values from 3 independent experiments. *** P<0.001. 