(C) Brains from w1118 and alix1 larvae were stained with anti-acetylated tubulin (green), anti-Cnn (white), anti-α-tubulin (red) and Hoechst (blue). Scale bars, 5 μm. The fluorescence intensities of acetylated MT, total MT and acetylated MT relative to total MT of 66 w1118 NBs and 65 alix1 NBs obtained from three independent experiments is shown graphically (±S.E), *p<0.05 and **p<0.01 (Student's t-test) 