 (C) PLA signal (green) for Hrs/Tau interaction (arrows) on an mCh-Hrs-labeled endosome (red) in N2a cells; scale bar: 5 μm (see also Fig. EV1). (D) Super-resolution images of N2a cells transfected with constitutively-active Rab5 (EGFP-Rab5-CA) and immunostained for Tau, revealing the presence of Tau in both membranes and lumen of early endosomes (scale bar: 5 μm) 