(D) Mutating LC8 binding consensus sites within Bim disrupts the Bim-Beclin 1 interaction. Bim(EL) EE/vector (IP negative control), Bim(EL) EE/Beclin 1-Flag, Bim(EL) EE-S109A, S113A, T114A (AAA)/Beclin 1-Flag, or Bim(EL) EE-T116E/Beclin 1-Flag were transfected into HeLa cells. Anti-Flag (M2) was used for immunoprecipitation. Data are shown as mean ± SD. ∗∗∗p < 0.001. 