(C) YTS KIR2DL1 cells were pretreated either SHP-1 and Cbls siRNA or N.S siRNA loaded NPs. After 48 hours, cells were incubated with either 721.221 Cw4 or Cw7 target cells for 2 hours and analyzed by flow cytometry to determine the expression of CD107a. Expression of CD107a was quantitated by mean fluorescence intensity (MFI). Data are means ± SEM of nine independent experiments. .) (n=9). P values were calculated by One-sample t-tests and One way ANOVA following Tukey's post hoc analysis and are indicated by asterisks. **P < 0.01. 