Quantification of nucleo-cytoplasmic (N/C) intensity ratio based on immunohistochemistry of WT-mCherry (n=45) and MUT-mCherry (n=63) tagged TDP-43. Each dot represents one analysed cell, unpaired Mann-Whitney-test. Western blot showing untagged TDP-43 (left panel) and phosphorylation state of the untagged allele (right panel) in WT -and MUT-mCherry motor neurons and in I ratio quantification of normalized pTDP43/normalized TDP-43 (because of almost the same molecular weight two different blots were used), ratio paired-t-test. 