C Plot of the METRIS rolling parameters of UBE2R2 WT, D143K, and R149E compared using one-way ANOVA (**** p ≤ 0.0001, WT: 63 beads x 17 rolls n=1071, R149E: 46 beads x 17 rolls n=782, D143K: 47 beads x 17 rolls n=799; technical replicates), indicating a weaker interaction with Ub between the UBE2R2 variants compared to WT. Error bars: SEM. 