(F). Pseudouridylation activity of TruB1 for tRNA and pri-miRNAs. 32p-UTP-labelled tRNAphe, pri-let-7a1 or pri-miR-10a were treated with recombinant TruB1. Upper bands represent uridine (U), lower bands represent pseudouridine (Ψ) in autoradiographs of the TLC plate. 