(D) The ab initio shape envelopes of the 3'SLs of DENV2 (left), ZIKV (middle) and WNV (right), WNV-3'SLM (green). The atomic models from de novo structure modeling by Rosetta except for 3'SLM of WNV are superimposed onto the respective envelopes. The back-calculated scattering profiles of the de novo atomic models are fitted to the respective experimental scattering profiles in (A). (E) Illustration of the three binding sites (orange asterisks) of WNV- 3'SL to eEF1A (pink ellipse), the potential pseudoknot is also indicated with red dash line. (F) The structure of ribosome-bound eEF1A (PDB: 5LZS) is compared with the envelope and atomic model of WNV- 3'SL side by side. 