c. Pseudo-colored PIN2-GFP signal in epidermal cells of 5 DAG old roots 12 HAT to ammonium or nitrate containing media. White arrows point to PIN2-GFP containing intracellular vesicles. Box plots represent the ratio in fluorescent signal detected inside the cell vs on the membranes (FI[cyt]/FI[m]). n=6 roots per condition, 5 cells per root were analyzed. Scale bar=5µm. The statistical significance was evaluated with ANOVA at p<0.05. The box chart components are defined as; box (25%-75%), central band (median line), central box (mean) and the range is within 1.5IQR. 