MCF10A cells were treated with either EtOH, 25uM 4HNE or 5uM RSL3 for 60 minutes. Isolated protein was assessed by immunoblotting for 4HNE, prominin2, and β-actin expression. Shown is one replicate of three independent experiments (n=3). MCF10A cells were treated with DMSO or 100uM MDA for 60 and 120 minutes. Isolated protein was assessed by immunoblotting for prominin2, and β-actin expression. Shown is one replicate of three independent experiments (n=3). 