A. Gcn5 binding to chromosomes assessed by chromosome spreading. Fission yeast nda3-KM311 cells were shifted at 19°C for 4 hours to enrich the population for the early stages of mitosis, and processed for chromosome spreading and immunofluorescence microscopy against Gcn5-myc and Cut14-HA. The Cut14/DAPI ratio served as marker for mitotic progression, with interphase (i): ratio<0.5; prophase (p): 0.51.5. For each strain, four representative nuclei extracted from a same image acquired with same settings are shown. Bar: 5 µm. Boxes indicate the 25th, median and 75th percentile and whiskers the min and max values (n>150 nuclei per strain). 