 (A, B) The relative spindle angle between the polarity axis (perpendicular to the apical surfaces stained wit Bazooka (B)) and spindle axis (defined by γ-tubulin-stained centrosomes) was determined in wild type (w1118) and alix NBs (B) incubated at room temperature (RT) or ice for 40 minutes. The graphs show the percentage of FECs or NBs displaying relative spindle angle 90-75° versus 75-0° or 0-15° versus 15-90°, respectively. The graphs represent the results from three separate experiments analyzin 32 w1118-NBs-RT (dark blue bars), 64 w1118 NBs-ice (light blue bars), 56 alix1 NBs-RT (dark grey bars) and 63 alix1 NBs-ice (light grey bars) (B 