E. Featured molecules in each cluster were determined and visualized by heatmap. The values of each molecule were normalized by Z-score. Molecular features of each cluster suggested that clusters 0 and 1 were GCP lineage cells, cluster 3 was GCs and clusters 2 and 4 were likely to be interneuron precursors as Pax3 and Id3 expression were strong. In addition, the strong and featured expression of Atoh1 suggested that cluster 0 and cluster 1 might be AT+GCPs and ND+GCPs. 