B-F. AT-ND transition in cultured GCPs. (B-E) GCPs were extracted from P5 (P5 GCPs) or P10 (P10 GCPs) mouse cerebella and immunostained with anti-Neurod1 (green) and anti-Ki67 (magenta) at DIV1 and DIV2. (F) Proportions of NEUROD1- cells (AT+GCPs) among total KI67-positive cells. Bars and error bars are representing as Mean ± SEM. Tukey-Kramer test was performed. **p <0.01, N.S.: Not Significant. Number of cells: 95,194 and 53 cells from N=3 mice (P5DIV1), 543, 238 and 550 cells from N=3 mice (P5DIV2), 121, 242 and 232 cells from N=3 mice (P10DIV1), 110,137 and 210 cells from N=3 mice (P10DIV2). 