(F) Bim T116 phosphorylation disables Bim-Beclin 1 interaction. Bim(EL) EE /vector (IP negative control), Bim(EL) EE/Beclin 1-Flag (in duplicate), or Bim(EL) EE-T116E (phospho-mimic, designated as T116E here)/Beclin-Flag (in duplicate) were transfected into HeLa cells. After 20 hr, one set of Bim(EL) EE/Beclin 1-Flag transfections and one of Bim(EL) EE-T116E/Beclin 1-Flag transfections were starved for 2 hr in HBSS (Starv). Anti-Flag (M2) was used for immunoprecipitation. 