(A) The effect of overexpression of the indicated TRs (Nanog, Irf6, Tfap4 and Irf6+Tfap4) on the expression of the other TRs of the network was determined by RT-qPCR analyses. Dark blue color denotes TRs upregulated more than 2 fold. Reduction by more than 2 fold in the expression is depicted by the absence of the corresponding TR. The light red and blue colored circles indicate downregulation or upregulation in the range of 1.3-2 fold, respectively, while grey circles indicate no change of gene expression. O: Oct4, S: Sox2, K: Klf4 and M: c-Myc. The light grey-green and red boxes depict the upstream and downstream layers of the 9TR GRN. The first panel on the left denotes the native 9TR GRN as shown in figure 4B (Phase III). 