A. P5 or P10 mouse cerebella were electroporated with H2B-GFP plasmids and immunostained with anti-Atoh1 (magenta) the following day (P6 or P11). Nuclei of electroporated cells were detected by GFP (green). Arrows indicate the cells of interest where GFP signals are co-localized with ATOH1. The proportions of ATOH1-positive cells(AT+GCPs, A'). Bars and error bars are representing as Mean ± SEM. Student T-test was performed between P6 mice electroporated at P5 (P5-6) and P11 mice electroporated at P10 (P10-11). ***p < 0.001. Number of cells: 108, 72 and 104 cells from N=3 mice (P5-6), 182, 45 and 61 cells from N=3 mice (P10-11). 