F, Selection of the most profoundly altered metabolites in extracellular spent growth medium from normal (normal and WT, n=4 and n=2, respectively), tumor (n=4), and residual (n=4) populations, based on metabolomics analysis targeted at central carbon metabolites. Values represent metabolite abundance levels as quantified by the area under the curve (AUC) of the marker fragment ions/transitions for each metabolite. Statistics were calculated using the limma package in R (Ritchie et al., 2015). Significant results (marked with *) correspond to a Benjamini-Hochberg adjusted p-value ≤ 0.05 (residual or tumor compared to normal). ns, not significant. Box plots: midline, median; box, 25-75th percentile; whisker, minimum to maximum. Data information: Number of replicates corresponds to individual mice used to derive organoids. 