E HeLa cells with stable inducible E2F7/8-EGFP were imaged by fluorescence and differential contrast (DIC) microscopy at 3 minute intervals. Cells were treated with CDH1 siRNA for 10 hours, synchronized at the G1-S border by 16h thymidine treatment, followed by thymidine release and induction of E2F7/8-EGFP by doxycycline. Mean integrated fluorescence of the cells was measured and normalized to the intensity in the frame of nuclear envelope breakdown (NEBD) (set at 100%), as determined by cytoplasmic dispersal of the fluorescent signal. The x-axis is set to 0 at the onset of anaphase, as observed in the DIC channel. Graphs shown are mean±s.e.m. Left graph: control n=15, Cdh1 RNAi n=14 both from 3 independent experiments. Right graph: control n=13, Cdh1 RNAi n=13 both from 2 independent experiments 