B) Fluorescence micrographs of germlines from animals expressing GFP::ZNFX-1 and PGL-1::TagRFP. The three panels on the left show about a dozen pachytene stage nuclei from a control (starting strain) animal, or the three classes of mutation identified in the genetic screen. Scale bar, 5μm. For class III images, nuclei are outlined with a dotted white line for clarity. The far right panel (GFP::ZNFX-1 inset) shows a blow-up of one nucleus/granule from each genotype to better illustrate the Z granule defects. Scale bar for insets: 2μm. 