(C) Mutating LC8 binding consensus sites within Bim disrupts the Bim-LC8 interaction. Bim(EL) EE/vector (IP negative control), Bim(EL) EE/Myc-LC8, Bim(EL) EE-S109A, S113A, T114A (AAA)/ Myc-LC8, or Bim(EL) EE-T116E/ Myc-LC8 were transfected into HeLa cells. Anti-Myc (Rabbit) was used for immunoprecipitation. Data are shown as mean ± SD. ∗∗∗p < 0.001. 