Quantitative analysis of migration distance, velocity, and directionality of Bu-HFSCs with the indicated genotypes (n=3 pairs; each biological replicate is an average of 3 different colonies with 8 cells tracked per colony) CTL, control; Ovol2 K DKO, Ovol2- and Zeb1-deficient Step-length comparison among the indicated genotypes. Kolmogorov-Smirov test Ovol2 KO (E), p<10-10; CTL vs. DKO (E), p=0.06 Straightness distribution comparison among the indicated genotypes. Kolmogorov-Smirov test Ovol2 KO (F), p<10-10, p<10-5; CTL vs. DKO (F), p=0.45 