A) gfp::znfx-1; pgl-1::tagrfp animals were mutagenized with ethyl methanesulfonate (EMS). F2 progeny of mutagenized animals were fed bacteria expressing dpy-11 dsRNA. Mutant animals that failed to inherit dpy-11 RNAi (were non-Dumpy) were isolated. Lineages established from these animals were examined under a 63X oil objective on a Zeiss Axioplan fluorescence microscope for defects in number, size, morphology or subcellular distribution of Z granules using GFP::ZNFX-1 as a marker of Z granules. 