(I) 0.5x106-2x106 indicated tumor cells were injected subcutaneously in NOD.Cg Prkdcscid Il2rgtm1Wjl/SzJ animals with Matrigel in PBS. When tumors appeared on animals (3-4 weeks), animals were i.p. injected with indicated DR5 agonists (4-6 doses), followed by tumor extraction and preparation of single cell suspension isolation from tumors after indicated antibody treatments. 