C. A Venn diagram of the differentially expressed genes (DEG) among VcdRP (red), VcdR (yellow) and VcdP (blue). RNA samples were harvested from V. cholerae ΔvcdRP strains carrying pBAD-based plasmids depicted in (A), including an empty vector control. Genes with a total count cutoff >10 in all samples, with an absolute fold-change ≥ 2.0 and a FDR adjusted p-value ≤ 0.05 were considered to be differentially expressed. 