(F) Working model for the recruitment and regulation of Ykt6 during autophagy. Ykt6 arrives either after prenylation of via COPI-coated vesicles at the ER, where it binds the Dsl1 complex. Ykt6 is packaged into COPII-coated vesicles, which deliver Ykt6 independently from Atg9 vesicles to the PAS. Atg1-Atg13 kinase controls Ykt6 activity until autophagosomes are completely closed. 