 (A, B) Representative images and quantification of 14 DIV neurons transduced with mCh, mCh-TSG101, or mCh-Rab35, treated for 5 h with either DMSO (CON) or Bafilomycin (Baf) to block lysosomal degradation, and immunostained for Tau and LAMP1. Overexpression of Rab35 or TSG101 increases Tau accumulation in lysosomes of Baf-treated neurons, indicating that Rab35/ESCRT pathway activation stimulates sorting of Tau into lysosomes (scale bar: 5 μm)(n=25-27 cells/condition; for TSG101, 2-way ANOVA, Baf x TSG101 interaction F1, 99=4,075 p=0.04, overall Baf effect F1, 99=10,30 p=0.0018, Sidak posthoc analysis ***p=0.0007; for Rab35, 2-way ANOVA, Baf x Ra35 interaction F1, 101=5,36 p=0.02, overall Baf effect F1, 101=12,01 p=0.0008, Sidak posthoc analysis ***p=0.0001). All numeric data represent mean ± SEM 