D-E. Chromosomal occupancy of acetylated H3 during mitosis. Cells expressing Cnd2-GFP were blocked in early mitosis, mitotic indexes measured (D), and processed for ChIP against total H3, H3K9ac or H3K18ac. Heterochromatin (dh1) served as negative control. H3ac/H3 average ratios and s.d. calculated from 3 ChIPs on 3 biological replicates are shown. *** P<0.001, * P<0.05, °P>0.05. High-occup. and Low-occup. refer to condensin high-occupancy and low-occupancy binding sites, respectively. 