E, F MICAL2PV mutants lacking the MO domain does not inhibit TNT formation. Stable shM2 H1299 (E) or A549 (F) cells were transfected with the corresponding MICAL2PV mutants lacking MO domain or control vectors (Ctr). Following transfection, Western blotting was carried out using an anti-HA antibody to detect expression of the mutant proteins, and confocal microscopy was performed to examine TNT formation. 10 images per group were captured for quantification. Three independent experiments were conducted. Data information: Data were analyzed using a one-way ANOVA with Bonferroni post-test and shown as mean ± SEM; * P < 0.05, ** P < 0.01, *** P < 0.001, ns, not significant. 