D MICAL2PV interacts with N-GTPase, EF hand and C-terminal domains of Miro2, but not with the C-GTPase domain. A schematic representation of protein domain organization in Miro2 and its truncation mutants was shown in (panel a). H1299 cells were co-transfected with Flag-MICAL2PV and different Myc-tagged Miro2 mutants. Transfected cells were lysed, and immunoprecipitation was performed using an anti-Myc antibody and subjected to Western blotting with different antibodies as indicated (panel b). It should be noted that the upper band (marked by "*") migrating closely above the Flag-M2 band in the top section of panel b is a non-specific species because it is present in anti-Myc immunoprecipitates of cells only expressing the vector control, not expressing any Myc-Miro2 (lane 1). 