(C-D) Immunofluorescence microscopy of spinal cord of Fus+/+ (C) and FusΔNLS/ΔNLS (D) mice showing active-caspase 3 (green), ChAT (red) and DNA (cyan, DRAQ5).(E) Quantification of caspase 3 (Cas3)/ChAT/DRAQ5 triple positive cells in FusΔNLS/ΔNLS mice. N=7 per genotype, (**) p<0.01, by Student's unpaired t-test. 