E-H. Predicted base-pairing sequences for ptsG (E), treB (F), nagE (G) and ptsHI (H) to VcdRP. The numbers indicate the distances relative to the TSS for VcdRP, and the start codons of the respective mRNA sequences. Underlined is the conserved stretch of four consecutive cytosines that corresponds to the seed region indicated in (1C). The M2* and M2 mutations refer to the site-directed mutagenesis of two guanines/cytosines in positions 2 and 3 of the seed region to two cytosines/guanines in the corresponding mRNA/VcdRP, respectively. 