B Smurf gut barrier assay in anesthetised, live larvae. Left, control larvae, right, larvae overexpressing Atg16B in the gut enterocytes under control of the NP1-Gal4 driver. While the distribution of food in the gut of Atg16B-expressing larvae was different from controls, there was no leakage of dye from the gut in any of the animals in three independent experiments (each with n=30 larvae for each genotype). 