A) On monopolar spindles, as on bipolar spindles, chromosomes segregate in meiosis I, and sister chromatids in meiosis II. B) Model of how separase-dependent kinetochore individualization primes bivalents for deprotection of centromeric cohesin. If separase is absent in meiosis I, arm cohesin and not centromeric cohesin is removed in meiosis II. Under these conditions, sister kinetochores individualize in meiosis II instead of meiosis I, demonstrating that step-wise cohesin removal (first arm cohesin, then centromeric cohesin) depends on the kinetochore structure. Without kinetochore individualization, centromeric cohesin cannot be cleaved in the subsequent division. 