A, B. Cycloheximide (CHX) assay. (A) N2a cells transfected with Atoh1-GST, Ccnd1 and Cdk4 plasmids 2 days prior were harvested after 0, 4 and 8 hr treatments of 50ug/mL CHX and lysates were subjected to immunoblotting with anti-Atoh1 or β-actin as a housekeeping control. (B) Quantification of relative levels of ATOH1 to the β-ACTIN. N2a transfected with only Atoh1-GST is referred as WT and N2a transfected with Atoh1-GST, Ccnd1 and Cdk4 plasmids is referred as 4D. Same experiments were performed three times. Error bars are representing as Mean ± SEM. 