A Identification of parental origin of the chromosomes was distinguishable based on H2B-mCherry fluorescent intensities (paternal chromosomes exhibit lower intensities; n = 20 oocytes). Bars and error bars denote means ± SD. The z-projection image shows major satellite-mClover (centromeres, green) and H2B-mCherry (chromosomes, red). Time from anaphase onset is shown in h:min. Scale bar = 4 μm. The 3D-reconstructed image shows maternal (magenta) and paternal (cyan) chromosomes. Spots indicate centromeres. 