E. Lysates of N2a cells transfected with either WT or S328A were immunoblotted with anti-Atoh1 or β-actin. Quantification of relative levels of ATOH1 to the β-ACTIN (right panel). Bars and error bars are representing as Mean ± SEM from three trials of each condition. Student T-test between WT and S328A was performed. Three slots of each experimental condition indicated the biological replicants. 