(D) Heatmap depicting the mean expression fold changes compared to wild-type expressions for motif knockout and substitution with positionally or functionally equivalent motifs from other architectures. Constitutive and developmental promoters are highlighted in red and green, respectively. (E) Boxplot depicting the effects of INR being substituted by INR2 in cas and CG8157 (all measurements in these two core promoter constructs were pooled together; Wilcoxon rank-sum test **p = 0.0051 for comparing substitution with knockout (significant) and p = 0.17 for comparing substitution with wild-type (not significant). The middle hinge represents the median. The Interquartile range the difference between the 75th and 25th percentiles. 3-4 biological replicate measurements. 