 (C) Images of 14 DIV neurons transduced with mCh or mCh-Rab35, treated for 24 h with either DMSO (CON) or cycloheximide (CHX) and immunostained for Tau; scale bar: 10 μm. (D) Tau fluorescence intensity is markedly reduced in both soma and axons of CHX-treated neurons overexpressing Rab35 compared to mCh, indicating faster Tau degradation (n=24/condition; for axons, n=25-26/condition); for soma, 2-way ANOVA, overall CHX effect F1, 92=19.27 p<0.0001, Sidak posthoc analysis *** p=0.002; for axon, 2-way ANOVA, CHX x Rab35 interaction F1, 97=13.27 p=0.0004, overall CHX effect F1, 97=14.34, p=0.0003, Sidak posthoc analysis **** p<0.0001) 