(D-G) Flow cytometry dot plots of cells in dLNs from mice 7d post immunization that received the indicated transduced CD4OT-II and BHEL cells. Adoptively transferred cells were identified and distinguished from endogenous cells of recipient SMARTA mice (CD45.1+) by staining for CD45.2. For quantification of absolute cells numbers, precision beads were used and cells double positive for B220 and CD45.2 (D, BHEL cells) or CD3 and CD45.2 (F, CD4OT-II cells) analyzed. (E, G) Quantification of absolute BHEL cells (E) as shown in D and CD4OT-II cells (G) as shown in F. Shown are mean values with SD of 6 mice from 3 independent experiments. Each dot represents one animal. 