C BAK cleaved within its α1-2 loop by thrombin is able to mediate liposome permeabilisation. Recombinant BAK-6Hthrombin pre-cleaved or not with thrombin (see Fig EV4) was incubated with liposomes at either 20 or 60 nM and liposome permeabilisation induced by cBID was monitored over time. Data is presented as percentage liposome permeabilisation relative to detergent-lysed liposomes, mean +/-SEM of 3 independent experiments. 