Figure 2: FMN2 is highly expressed in mossy fibers and is required for synaptic depotentiation.E. Left panel: Representative images showing the co-localization of FMN2 (green) and Synaptoporin in the mossy fiber pathway in low and high magnification. Right panel: Immunoblot analysis of hippocampal synaptosomes (Syn) compared to cell homogenate (H) showing that FMN2 is present in the pre-synaptic compartment. Synaptophysin was used to confirm enrichment of synaptosomes. For all experiments parasagittal brain slices from 14-21 day old mice were used. Cere, cerebellum; Hip, hippocampus, mf; mossy fiber, str. Luc.; stratum lucidum, cc, corpus callosum. 