A) Super-pedigree mapping of two mutations in Pacs1 that were linked to peripheral B cell deficiency. Insert shows peripheral B cell deficiency in the endive (N=22 REF, 28 HET, 10 VAR) and chicory (N=24 REF, 15 HET, 3 VAR) pedigrees. Horizontal bars indicate mean values. Ordinary one-way ANOVA with Tukey's multiple comparisons test. Ns = not significant, **P < 0.01, and ***P < 0.001. Protein domain model shows the encoded location of the ENU alleles. 