(F) A representative Western blot of ovaries from w1118 and Dcp-1Prev1 flies that were subjected to fed or starvation conditions for 4 d. Ref(2) P was detected by immunoblotting. Actin served as a loading control. Densitometry was performed to quantitate Ref(2) P protein levels relative to actin. Graph represents ± SD from five independent experiments (n = 5) 