A, B Representative bright field images of TNTs in H1299 (A) and A549 (B) cells (black arrow). These TNTs were actin-enriched, as shown by phalloidin staining (red) and not attached to the substrate (Y-Z axes). Scale bar: 20 μm. Data information: nuclei were stained with Hoechst 33342 (blue). Boxed areas are enlarged and shown in panels on the right. 