(A) Larval NSCs of control (ctrl; yw) and pr-set7716 (716) at 24h ALH were stained for Deadpan (Dpn) and Miranda (Mira). (B, C) Quantifications of cellular extensions (B) and NSC diameters (C) for genotypes in (A). In (B), n=1055, 12 brain lobes [BL] for control; n=1000, 15 BL for pr-set7716. In (C), n=101 for control; n=112 for pr-set7716. Data information: Quantification data are presented in as mean ± SD (B, C n numbers in (B, were total NSCs counted with total brain lobes measured, while each brain lobe considered as one biological replicate. White arrows point to NSCs and yellow arrowheads point to cellular extensions of NSCs. Statistical analyses were done comparing two different genotypes using Mann-Whitney test (B, C, ***P < 0.001. Scale bars, 5 μm (A, 