(B) Representative light microscopy images of spinal cord sections of Fus+/+/ChAT-CRE (B1, B4), FusΔNLS/ΔNLS/- (B2, B5) and FusΔNLS/ΔNLS/ChAT-CRE (B3, B6) mice at birth stained with cresyl violet (Nissl, B1-B3) or anti-choline acetyl transferase (ChAT, B4-B6).(C-D) Quantification of Nissl+ (C) and ChAT+ (D) motor neurons per spinal cord ventral horn. N=9 Fus+/+/ChAT-CRE, N=8 FusΔNLS/ΔNLS/- and N=4 FusΔNLS/ΔNLS/ChAT-CRE; for Nissl+ and N=11 Fus+/+/ChAT-CRE, N=7 FusΔNLS/ΔNLS/- and N=8 FusΔNLS/ΔNLS/ChAT-CRE; for ChAT+ (**) p<0.01 vs Fus+/+, (##) p<0.01 vs Fus∆NLS/+ ; (ns) non-significant; One way ANOVA followed by Tukey post hoc test.(E) Total numbers of caspase 3 (Cas3)/ChAT/DAPI triple positive cells in Fus+/+/ChAT-CRE, FusΔNLS/ΔNLS/- and FusΔNLS/ΔNLS/ChAT-CREmice. N=9 Fus+/+/ChAT-CRE, N=7 FusΔNLS/ΔNLS/- and N=8 FusΔNLS/ΔNLS/ChAT-CRE; (**) p<0.01 vs Fus+/+, (#) p<0.05 vs Fus∆NLS/+ ; (ns) non-significant; One way ANOVA followed by Tukey post hoc test. 