A Dynamic SILAC, heat treatment and recovery. K562 human cells were grown at 37°C in light SILAC medium. 90 minutes before heat treatment the medium was changed to heavy SILAC containing stable heavy carbon and nitrogen isotopes in arginine and lysine amino acids. These heavy amino acids are incorporated into newly synthesized proteins while the light versions remain in pre-existing proteins. A control sample was collected (pre-shock) prior to partitioning cells for heat treatment. The cells were treated either with heat shock (44°C) or mock shock (37°C) for ten minutes. After heat treatment, cells were allowed to recover at 37°C and samples were collected at different time points. 