a Differential gene expression analysis shows a set of 196 genes being regulated by CHIR in d4TCF cells; several of these changes only appear when TCF/LEF proteins are not present (dark green set). Of the 134 genes that are not regulated by CHIR in dBcat cells (putative β-catenin-dependent genes), 107 require the removal of TCF/LEF factors, in that their regulation only occurs in d4TCF cells. The other 27/134 β-catenin-dependent transcriptional changes match the TCF/LEF-independent target genes previously described in Figure 3a. Their regulation occurs both in the presence and in the absence of TCF/LEF factors. 90/107 of the putative β-catenin-dependent genes, stop being regulated by CHIR in pentaKO cells (β-catenin-GHOST, blue box) 