(D) Immunoblot analysis of FUS protein in cerebral cortex of 2 Fus+/+, 2 FusΔNLS/+ and 2 FusΔNLS/ΔNLS knock-in mice using a combination of two different antibodies targeting either the C-terminal (C-ter. 1 and C-ter. 2) NLS, the N-terminal part (N-ter. 1) or an internal part (N-ter. 2) of FUS. Molecular weight markers are shown on the left, and apparent MW is indicated. 