C. Condensation assay. 3D distances between two fluorescently-marked loci on chromosome I were measured by live cell microscopy in fission yeast cells progressing from late G2 through mitosis. Time lapse recording for n>32 cells for one (wild type) or two (gcn5-47 and cut14-208) biological replicates were aligned to anaphase onset (t = 0) and average distances (± s.d.) plotted. Values are listed in Appendix Table S1. 