C MAD2 kinetochore localization is defective on spermatocyte-derived chromosomes. The localization of the closed (active) form of MAD2 (C-MAD2, green) was investigated as in (A). Note that the kinetochore enrichment of MAD2 was less on the chromosomes in half of the metaphase plate, which likely corresponded to spermatocyte-derived chromosomes, in control and halved oocytes. 