(p) Glycolysis is essential for hematopoietic differentiation of HE cells and inhibiting pyruvate entry into mitochondria (via UK5099 or shMPC1/2) favors a primitive erythroid fate. Increasing pyruvate flux into mitochondria (via DCA or shPDK4) amplifies acetyl-coA production which fuels cholesterol biosynthesis and promotes definitive hematopoietic differentiation of HE cells. 