(C) Control co-transfected vector (backbone not represented) used for data normalization (Material and Methods), and consisting in a pTran promoter driving the expression of the Renilla Luciferase gene. (D) Simplified dual luciferase assay experimental workflow. To measure promoter activity quantitatively on a large scale with high reproducibility we integrated the golden gate cloning strategy (BsaI cloning) with a high-throughput experimental pipeline using automated robot systems for colony picking, reporter plasmids isolation, transient co-transfection and dual luciferase assay (Figure EV1 and Materials and Methods for details). ( 