Schematic for Rfa1 and Rfa2 ChIP-Seq (this Figure) and BrdU IP-Seq (Fig 6). Yeast cells were arrested with α factor at G1 phase, then released into fresh YPD medium containing BrdU to label newly synthesized DNA as well as to allow cell enter S phase at two conditions: (i) in medium with 0.2 M HU for 45 min at standard growth temperature (25 oC), (ii) in medium without HU for 72 min at low temperature (16oC) to slow down S phase progression. Cells were then collected for Rfa1/Rfa2-ChIP, or BrdU-IP. The resulted DNAs were detected by sequencing. 