D: 3D rendering of the aggregate shown in (C). Amorphous aggregate material is labeled in red, proteasomes in violet, ribosomes in yellow, TRiC/CCT chaperones in green, mitochondria (Mt) in white, ER in pink and other vesicles (V) in light yellow. The irregular aggregate structures were approximately segmented using a threshold-based approach for visualization purposes. Average structures of proteasomes, ribosomes and TRiC/CCT were pasted at the locations and orientations determined by template matching. 