Figure 2. Dynamic reorganization of chromosomes during replication. (D) Left panel: p(s) of replication defective cells (cdc6 thermosensitive mutant). G1 elutriated cells were released for 3h and 6h in non-permissive conditions. The corresponding FACS profiles show no S-phase progression. Middle panel: p(s) of cells that completed replication. G1 elutriated cells were released in S phase in the absence or presence of nocodazole (G2/M noc). Right panel: p(s) of cohesin depleted (ssc1 G2) and nocodazole arrested cells. 