(D) TBK1 does not phosphorylate HTTex1 in inclusion/ fibrils or promote their clearance/ disaggregation. When HTT was already aggregated in cells or when in fibril form in vitro, possibly S13 residue is not accessible for TBK1 phosphorylation as N17 was shown to be collapsed on fibrillar core or it is near the core. Also, TBK1 mediated autophagy flux was not able to induce the lowering of HTTex1 inclusion. There was no disaggregation of fibrils up on TBK1 kinase in vitro. 