E. Calculated percentage of double-positive vesicles (with >50% of the vesicle boundary demarcated by white signal in the colocalization analysis) from 20 cells counted from n=3 biologically independent experiments are shown. Each dot represents 1 cell in the dotted plot. The group means of % Rab10 or pT73-Rab10 vesicles positive with indicated markers were calculated and shown. Error bars represent± SEM. Significance was assessed by Mann-Whitney test for each marker with * representing p<0.05, ** P<0.01. 