(D) Splenocytes from Pacs1+/+ and Pacs1−/− mice were stained with cell surface antibodies to identify FOB cells and treated with 100 µM H2O2 for 35 minutes. Cells were then labelled with TMRE to monitor MMP by FACS analysis. Low TMRE fluorescence indicated susceptibility to H2O2 treatment. Data are presented as mean ± SD of 3-4 replicates, two-tailed unpaired t test, ***P < 0.001. Results are representative of three independent experiments performed on different Pacs1+/+ and Pacs1−/− pairs. 