Scheme of the experiment. C57BL/6N mice pups at P7 with nursing mothers were subjected to hyperoxic conditions leading to vessel obliteration in the central zone of the retina. At P12, mice pups were returned to room air, which induces vessel regrowth but also pathological neovascularization, characterized by pre-retinal neovascular tufts. At P15, mice pups were injected intravitreally with 1 μg control IgG-Fc (left eye) and Sema3C-Fc (right eye). Retinae were analyzed 2 days later after intravitreal injection. 