(B) Brains of w1118 larvae were stained with anti-ALIX (white), anti-Cnn (white) or anti-Asl (white) and Hoechst (blue) and their centrosomal intensities were calculated in 41, 44 and 40 metaphase NBs, respectively. The average ratios of centrosomal intensities (strongest/weakest) (±S.E) of ALIX, Cnn and Asl calculated from 77, 76 and 56 NBs, respectively, are indicated below the micrographs (from at least three experiments). Scale bars, 5 μm 