(C) RT-PCR analysis of brain from 2 Fus+/+, 2 FusΔNLS/+ and 2 FusΔNLS/ΔNLS knock-in P0 mice using primers located in the STOP cassette, and thus specific to the ΔNLS mRNA (ΔNLS, upper panel), or primers located in exon 11, i.e. upstream of the floxed cDNA insertion, and thus amplifying total Fus-derived mRNA (Total, middle panel). PCR amplification of 18S rRNA is shown as standard gene (lower panel). 