(C) Digitonin-solubilized (1 or 4 g/g digitonin/protein ratio, as indicated) mitochondrial membranes from the indicated yeast strains (wild type, pex19Δ, pex19Δmsp1Δ) expressing Pex13-V5 (under its endogenous promotor) were separated by BN-PAGE with a 3-18% gradient and immunoblotted for PEX13-V5 (α-V5). Green box for wild type, orange box for pex19Δ, purple box for pex19Δmsp1Δ, indicates gel area of 300-130kDa correlated to complexome profiling heatmap. (D) Heatmap representing the protein abundance of Pex11, 13, 14, 17, 25 in digitonin-solubilized (3 g/g digitonin/protein ratio) mitochondrial membranes from the indicated yeast strains (wild type, pex19Δ, pex19Δmsp1Δ) expressing Pex13-V5 (under its endogenous promotor). Green box for wild type, orange box for pex19Δ, purple box for pex19Δmsp1Δ, indicates gel area of 300-130kDa correlated to complexome profiling heatmap. (E) Heatmap representing the protein abundance of Mdh3 and Pot1 in digitonin-solubilized (3 g/g digitonin/protein ratio) mitochondrial membranes from the indicated yeast strains (wild type, pex19Δ, pex19Δmsp1Δ) expressing Pex13-V5 (under its endogenous promotor). 