D HeLa cells expressing inducible E2F7-EGFP (left destruction graph) or E2F8-EGFP (right destruction graph) were blocked with thymidine for 16h, then released in fresh medium with doxycycline. Imaging was performed at 3-min intervals, as in Figure 2E. The x-axis is set to 0 at the frame of anaphase onset. Graphs show mean ± s.e.m. wild-type (wt) shown from Figure 2E in gray. E2F7-EGFP-KENmut n=13 from 2 independent experiments, E2F8-EGFP K/Kmut n=26 from 3 independent experiments. 