(B) Heparan sulfates were removed from Huh7.5 cells by enzymatic treatment with heparinase III for 1 hour at 37°C, then washed, and exposed to SARS-CoV-2 pseudovirus for 4h at 4°C. Treated and untreated cells were subsequently lysed and binding was determined by ELISA. Data information: Data show the mean values and error bars are the SEM. Statistical analysis was performed using unpaired Student's t-test with Welch's correction. *P≤0.05, **P≤0.01(n=3) 