 (E) CHO tetracycline-inducible cells expressing Z α1-antitrypsin were induced with 0.5 μg/mL doxycycline for 48h and treated with 10μM GSK716. Cells are then lysed in 1% v/v NP-40 buffer at different time points (0, 12, 24, 36, 48, 60, 72, 84, 96 and 108 hours). For every time point, NP-40-soluble and insoluble fractions were separated and immunoprecipitated with the 2C1 mAb and separated by 4-12% w/v SDS-PAGE and α1-antitrypsin detected by immunoblotting. PRE indicates cells pre-treated for 48 hours with 10μM GSK716 and induced for the same time with 0.5 μg/mL doxycycline. The rate of clearance of soluble and insoluble polymer is shown 