(E) HBSS starvation increases Bim T116 phosphorylation. Bim(EL) EE or BimEE(EL)-T116A were transfected into HeLa cells. After 20 hr, one set of Bim(EL) EE transfections and one of Bim(EL) EE-T116A were starved for 2 hr in HBSS. Blots were probed with the indicated antibodies. 