F Immunoblots of FACS-sorted RPE cells with stable expression of the FUCCI system. Cells were sorted based on expression of truncated versions of and Azami Green-tagged Geminin (amino acids 1-130) and Kusabira Orange-tagged CDT1 (amino acids 30-120), respectively. Blots are representative examples of four independent replicates derived from two different stable RPE-FUCCI clones. 