A Schematic overview of protocols used to derive naïve-like hESC lines by 2a2iL from human blastocysts. Two protocols were used to generate naïve hESC lines. In protocol 1, isolated ICMs were cultured in hESCs medium that contained basic fibroblast growth factor (bFGF) for 4 days followed by 2a2iL-supplemented medium until day 10. Dome-shaped colonies representative of the naïve state formed after single-cell dissociation. In protocol 2, ICMs were exposed to 2a2iL-supplemented medium at the beginning of seeding. 