B, C, In primary endothelial cells isolated from human IGF-1 receptor endothelial overexpressing mice (hIGFREO) and wildtype littermates (WT), quantitative polymerase chain reaction (qPCR) shows that hIGFREO have increased expression of human IGF-1R but similar levels of murine insulin receptor (IR) gene expression as WT (n=3-5 mice per group). 