, C TNT formation was quantified in transfected cells following confocal microscopy. Expression of MICAL2PV mutants, #1, #2 or #4 significantly reduced TNT formation Three independent experiments were conducted. Data information: Data were analyzed using Student's t-test and shown as mean ± SEM; * P < 0.05, ** P < 0.01, *** P < 0.001, ns, not significant. 