E BMMΦ were infected with BCG-RFP (MOI15) for 6 days, fixed and stained for nitrotyrosine (green) and DAPI (blue). Representative image of reconstruction from indicated area and quantification of intracellular nitric oxide to BCG-RFP colocalization; scale bar, 20µm. Box plot of >30cells with mean ± SEM of 2 independent experiments. 