(D) Co-localization analysis by epifluorescence microscopy of Sec24-mRFP and de novo made UapA-GFP (8h derepressed transcription). Quantification of co-localization was made by calculating Pearson's Correlation Coefficient. For the statistical analysis one sample t-test was used. Notice the significant co-localization (PCC=0.61±0.07, ****P<0.0001). Due to the partially functional mRFP-tagged version of Sec24 (evident in the growth test shown on the left panel), UapA trafficking is significantly delayed, reflected in the appearance of prominent labeling of uniform perinuclear ER. Scale bar: 5 μm. Biological/technical replicates: 3/5. 