D) Hits mapped onto the 187 human genes used as the basis for screening in D. melanogaster. Protein-protein interactions were identified from the set of 409 proteins identified in mammalian cells[16]. For gene knockdowns that inhibited autophagy, dark red indicates that two amplicons had a score < -0.5, while light red indicates that only one amplicon had a significant SSMD score, but that it was < -1. For gene knockdowns that increased autophagy, dark blue indicates that two amplicons had an SSMD score > 0.5, while light blue indicates that only one amplicon had a significant SSMD score, but that it was > 1. 