B Single molecule tracking at high frame rate: the time of ttl = 10ms between two images allows to estimate the distribution of displacements, that is then fit by a three-component diffusion model to estimate the fraction of molecules immobilized on chromatin. Inset: Average bound fraction (see Fig. EV4C and Methods). Note that MycRA displays a significantly lower bound fraction than MycWT and MycHEA (ncells= 34, 30 and 35, and ndisplacements= 78550, 59783 and 83801 for MycWT, MycRA and MycHEA, respectively). Error-bar: SD. Statistical significance evaluated by permutation tests Data information: , the statistical significances are indicated relative to the WT control (or RA vs. HEA, as indicated): * P < 10-4; # P < 10-3; + P = 3.1x10-3; ns not significant 