F, Fluc-EGFP protein quantity measured in the indicated brain regions of Fluc-EGFP mice at the indicated ages. Values are normalized to the 3-month-old group. N=5 Fluc-EGFP mice for each age group. One-way ANOVA with Bonferroni's multiple comparisons test. ANOVA: Hippocampus, *p = 0.0278; Cortex, n.s; Cerebellum, **p = 0.0058. Significant pairwise comparisons to the 3-month-old group are indicated on the graphs. Protein quantity normalized to total protein instead of tubulin is shown in Appendix Fig. S2C. 