 (A, B) The relative spindle angle between the polarity axis (perpendicular to the apical surfaces stained with phalloidin (A and spindle axis (defined by γ-tubulin-stained centrosomes) was determined in wild type (w1118) and alix1 FECs (A incubated at room temperature (RT) or ice for 40 minutes. The graphs show the percentage of FECs or NBs displaying relative spindle angle 90-75° versus 75-0° or 0-15° versus 15-90°, respectively. The graphs represent the results from three separate experiments analyzing 33 w1118 FECs-RT (black bars), 44 w1118 FECs-ice (light grey bars), 38 alix1 FECs-RT (dark grey bars), 46 alix1 FECs-ice (white bars) (A 