F. Western blot analysis of Fn12230 (Fn), fadA-deletion mutant US1 (∆fadA) and spontaneous mutant lam following SDS-PAGE. A total of 400 μl of each culture grown to OD600 of 1.0 was pelleted and loaded onto 12% SDS-PAGE. FadA protein was detected using anti-FadA mAb 7H7 at 1:4,000 dilution. Compared to the wild type, lam produced significantly less pre-FadA, but not mFadA. 