A Protein expression of wild-type and KEN-mutant E2F7 and E2F8 in 293T cells in presence or absence of CDH1-flag 48 hours after transfection. For E2F8, KEN motifs starting at amino acid 5 (KEN5) or 374 (KEN374) were mutated separately into 3 consecutive alanines or in combination (K/Kmut). Blots are representative of two independent experiments. 