(E) Polarized Calu-3 were inoculated with 0.5 TCID/mL of a SARS-CoV-2 isolate (hCoV-19/Italy) either directly or in presence of antibodies against ACE2 or upon pre-treatment with LMWH enoxaparin (250 IU/mL) for 30 min at 37°C. Virus was detected by lysing after 48 hours through quantitative real-time PCR of viral RNA (E) and virus production was determined by detection of viral RNA in supernatant using quantitative real-time PCR (F). Data information: Data show the mean values and error bars are the SEM. Statistical analysis was performed using (E, F) ordinary one-way ANOVA with Tukey's multiple-comparison test. *P≤0.05, **P≤0.01, ***P≤0.001, ****P≤0.0001 (n=3 donors measured in monoplo). TEER: Transepithelial electrical resistance. 