MCF10A cells were treated with either 25uM 4HNE or 5uM RSL3 for times that ranged from 0 to 120 minutes. mRNA was isolated from each time point and HSF1 expression was quantified by qPCR. Shown is an average of three independent experiments with standard deviation (n=3 experiments per time point). P‑values were obtained by unpaired Student's t‑test with *P<0.05, **p<0.01, ***p<0.005. Exact p‑values are reported in the Appendix Table S1. 