D. Chromosome segregation in gcn5-47 or cut3-477 mutant cells. Cells growing at 32°C were fixed and processed for immunofluorescence microscopy against Mis6-HA and Cdc11-GFP to reveal centromeres (Cen) and Spindle Pole Bodies (SPB), respectively. DNA was stained with 4',6-diamidino-2-phenylindole (DAPI). Left panel: single mutant cells cut3-477 or gcn5-47 exhibiting chromatin bridges (b) or trailing chromatin (t). Bar: 5 µm. Right panel: frequencies of chromatin bridges and trailing chromatin as a function of spindle length (SPB-SPB distance, n> 40 for each category). 