C) Representation factor indicates the number of genes overlapping divided by the expected number of genes overlapping in two datasets, with a value of one (dashed line) indicating random overlap. A representation factor >1 indicates more overlap between datasets than expected by chance. Data show a comparison of lists of genes differentially targeted by endo siRNAs in zsp-1(gg682) animals relative to WT animals against lists of genes known to be targeted by CSR-1 (Claycomb et al. 2009), HRDE-1 (Buckley et al. 2012), WAGO-4 (Xu et al. 2018), or all WAGO-class endo siRNAs (Gu et al. 2009). 