(A) Heat maps representing enriched HCDR3 sequences across the different panning sets for the short- and long-HCDR3 (upper and lower panel, respectively) phagemid libraries. HCDR3 sequences were selected based on NGS counts in 100'000 analyzed sequences (Z-score values; Appendix Table S2) and clustered according to the NGS-binding profiles. Red and blue: high and low number of NGS counts of the HCDR3 sequence, respectively. Donut charts (the right side) of each heat map indicate the number of clones with NGS-identified HCDR3 for a predicted PrP epitope. (B) Bar graphs showing the number of rare (one count in mouse recPrP23‑231 panning) and predominant (count ≧ 20) clones binding to the various PrP regions for the short- and long-HCDR3 libraries. 