(C) Semi-quantitative RT-PCR analyses of selected targets. Left panels show representative acrylamide gel pictures of RT-PCR products. Quantification of splicing changes from at least three biological replicates of FusΔNLS/ΔNLS (blue bars) and Fus-/- (red bars) compared to their control littermates (Fus+/+, black bars) by semi-quantitative RT-PCR (middle panel) and RASL-seq (right panel) are shown. Error bars represent SEM. 