(C) Co-localization analysis and relevant quantification of strains co-expressing de novo made UapA-GFP (120 min after initiation of transcription) with RabE-mRFP, AP-1-mRFP or ClaH-mRFP. Scale bars: 2 μm. Quantification by calculating Pearson's Correlation Coefficient (PCC) shows clear non-colocalization of UapA with all the Post-Golgi markers tested, as confirmed by one sample t-test (PCC=0.31±0.08, 0.29±0.04 or 0.07±0.01, with ****P<0.0001, for RabE, AP-1 or ClaH, respectively). Biological/technical replicates: 3/7 for each strain. 