(G) YTS KIR2DL1 were incubated with NKp46 antibody coated-NPs loaded with FITC labeled siRNA. After 24 hours, the cells were stained with anti-NKp46 monoclonal antibody followed by staining with Alexa568-Fluor goat anti-mouse IgG secondary antibody. Then, the cells were analyzed by flow cytometry to determine the incorporation of the rhodamine-labeled NPs loaded with FITC-siRNA and NKp46 antibody staining. Cells that were positive for both rhodamine-labeled NPs and NKp46 antibody staining, were analyzed for the presence of fluorescent FITC-siRNA. 