(B) Scheme of the ReDDM tracing method (Antonello et al., 2015). This system uses two fluorescent transgenes with short- (membrane CD8::GFP, green) and long-term (nuclear RFP::H2B, red) stability and the Gal80 repressor (tubα1-Gal80ts) for temporal control of UAS-driven transgenes. Using esg-Gal4, the reporter transgenes are seen in the ISCs and EBs (detected as double labelled cells). Newly renewed ECs or EEs are traced by the nuclear RFP owing to the protein stability of RFP::H2B. 