B) Volcano plot showing log2 fold change of the number of endogenous siRNAs targeting each C. elegans gene in zsp-1(gg682) vs WT animals on x-axis and log10 (adjusted p-value) of changes on the y-axis. Dots in red indicate siRNAs pools targeting this gene were significantly different in zsp-1(gg682) and WT animals. Dashed lines indicate log2(fold change)=-1 or 1. Adjusted P-value was calculated using Benjamini Hochberg method in DEseq2. 