(D) Control or ALIX depleted HeLa cells grown on coverslips were immunostained with anti-acetylated tubulin (green), anti-α-tubulin (red), anti-Pericentrin (white) and Hoechst (blue). Scale bars, 5 μm. The fluorescence intensities of acetylated MT, total MT and acetylated MT/total MT of 30 control and 28 ALIX-depleted HeLa cells is shown in the graph (±S.E) (three separate experiments). **p<0.01 by Student's t-test 