C Single molecule tracking at lower frame rate (ttlspanning between 200 ms and 2s) allows to quantify the distribution of residence times (i. e. the duration of binding events). Inset: Average residence times (see Fig. EV4E-F and Methods). The data reveal a significantly shorter average for MycRA, relative to either MycWT or MycHEA (ncells= 35, 35 and 31, and nBound − molecules= 2452, 2084 and 2171 for MycWT, MycRA and MycHEA, respectively). Error-Bar: SD. Statistical test: Anova-Tukey Data information: the statistical significances are indicated relative to the WT control (or RA vs. HEA, as indicated): * P < 10-4; # P < 10-3; + P = 3.1x10-3; ns not significant 