(A-C) Protein interactors of ANKFY1 (D.m. gene = CG41099), RABGAP1 and RABGAP1L (D.m gene = CG7112), RAB3GAP1 (D.m. gene = CG31935), and RAB3GAP2 (D.m. gene = Rab3-GAP), identified in mammalian mass spec. Note that each of these proteins interacts with an Atg8a ortholog. D. melanogaster orthologs of other protein interactors of ANKYF1 (D.m. genes Nipsnap and CG7053) were also identified as hits in the primary muscle screen. Based on our RNAi experiments red spheres are assigned to genes/proteins that promote autophagosome formation and/or expansion; blue spheres are assigned to genes/proteins that suppress autophagosome formation and/or expansion. 