(A) Total cell lysate (T), post-mitochondrial supernatant (PMS), and HistodenzTM-purified mitochondria (M+P, M) from the indicated yeast strains (wild type, pex19Δ, and pex19Δmsp1Δ) expressing Pex13-V5 (under its endogenous promotor) were separated by SDS-PAGE and immunoblotted for PEX13-V5 (α-V5) and porin (α-porin). 