(A) Experimental schemes LacI-CENP-B or LacI control (baits) were expressed into CENP-AEA/- lacO-tetO U-2OS cells. Immunostaining against CENP-C or CENP-A (preys) was performed following LacI-CENP-B transfection in untreated, IAA treated and IAA wash-out (WO) conditions. 