 JQ1 pre-treatment inhibits N9 microglial cell inflammation, proliferation, and migration. N9 microglial cells were pre-incubated with vehicle (DMSO), JQ1 (0.5 μM), RVX208 (30 μM), or Olinone (30 μM) for 12 h followed by stimulation with LPS (1 μg/ml) for another 2 h, and then subjected to qRT-PCR (a) for determination of inflammatory cytokine expression. Proliferation assay (b, BrdU ELISA) and migration assay (c, Transwell) were performed after incubation for additional 24 h. Quantification: mean ± SEM; n = 3 experiments; *P < 0.05, **P < 0.01 compared to vehicle + LPS 