(E‑F) Crosspriming of CD8+ T cells requires DC activation and type I IFN. (E) Representative dot plots showing the percentage of CD44high Kb‑OVA tetramer+ CD8+ T cells in the draining lymph node of WT and Irf3‑/‑Irf7‑/‑ mice injected with PBS, OVA or a mixture of OVA plus poly(I:C). (F) Flow cytometric quantification of CD44high H‑2Kb‑OVA tetramer+ CD8+ T cells in the draining lymph node of WT mice injected with PBS (grey dots), OVA (black dots), OVA plus poly(I:C) (blue dots) and in draining lymph node of Irf3‑/‑Irf7‑/‑ mice injected with OVA plus poly(I:C) (orange dots). Data are pooled from 2 independent experiments. 