EMBO/sd-character-level-ner huggingface dataset:
D miR-150 levels in IRE1α -/- MEF cells that were either untransfected (UT) or transfected with miRNA inhibitor control (siCtrl) or with a miR-150-5p miRNA inhibitor. * P = 0.034 compared to UT.
Proposed working model of CARM1-USP7-LSD1 axis involved in breast cancer metastasis. CARM1 methylates LSD1, which promotes its binding with and de-ubiquitylation by USP7, leading to LSD1 stabilization. The stabilization of LSD1 enhances the demethylation of H3K4me2 at E-cadherin promoter and H3K9me2 at vimentin promoter, resulting in repression of E-cadherin and activation of vimentin gene expression, respectively, which consequently promotes breast cancer metastasis.
(D) Left: number of Oct4-GFP positive colonies at day8 of reprogramming upon 48hours treatment with rotenone at day1-2 or at day5-6. Note that rotenone treatment given at day5-6 does not reduce the number of iPS colonies. Mean and s.e.m of two technical replicates is shown. See also Appendix Figure S10C. Right: representative Alkaline Phosphatase (AP) staining of iPSc colonies generated from GOF18 EpiSCs at day7 of reprogramming treated with DMSO (top) or with 48h of rotenone at days 1-2 (middle) or 5-6 (bottom).
44 healthy volunteers conducted a three week KD with a limited carbohydrate consumption of <30g per day. Blood was taken and analyzed prior to starting the diet (T0) and again after three weeks of strict adherence to the diet (T1). PBMCs were isolated. T cell stimulation was performed through CD3/CD28 Dynabeads at a bead:cell ratio of 1:8. Pan/CD4+/CD8+ T cells were separated via magnetic cell labeling. Mitochondrial metabolism was analyzed for each subpopulation using a Seahorse XF96 Analyzer. D Quantification of mitochondrial mass using MitoTracker green, indicated by MFI FITC in human CD4+/CD8+ T cells, n=11/8 individual human subjects. Data information: Data depicted as mean ± SEM (OCR) or as box plots with median, twenty-fifth and seventy-fifth percentiles and range Dots indicating individual values. *p<0.05, **p<0.01, paired t-test/ Wilcoxon matched-pairs signed rank test, as appropriate.
E Heatmap representing the enrichment and depletion of binding motifs of top 50 TFs in OCRs of T-ALLs in comparison to healthy T-cell precursors. Patients on the x-axis; same order as in panel (C). Gray cells represent TFs having non-significant enrichment or depletion.
(K) Cells from (J) were subjected to flow cytometry analysis. Annexin V-positive cell numbers were obtained from three independent experiments and normalized to those of controls cells treated with DMSO. Bars, means +S.D. from three independent experiments. For statistical analysis, student t-test (two-sided, one type) were used. * P value: 6 hrs, 0.0012; 17 hrs, 0.0041.
B, The lysates (T) were sonicated and centrifuged at 100,000 g for one hour to separate pellet (P) and supernatant (S). The distribution of CPY and Apg8p was examined by immunoblotting with each specific antibody, respectively.
C) Elucidation of protein-protein interaction between HP1 and H3K9 MTs by co-immunoprecipitation analysis. Whole-cell extracts of HEK293T cells co-expressing HA-HP1β mutants and epitope-tagged proteins of interest were subjected to immunoprecipitation with the anti-HA tag antibody. The immunocomplex was analyzed by immunoblotting with the indicated antibodies. The intensities were quantified using ImageJ software, normalized, and shown on the top.
Number of Us11-GFP expressing neurons at 3 days post-LY294002-induced reactivation in P6 SCG neuronal cultures transduced with shRNA targeting PML for 3 days prior to infection with HSV-1 in the presence or absence of IFNα (150 IU/ml). n=9 biological replicates.
E N-myristoylation of EGR2 in plants. Total proteins were prepared from two-week-old EGR2-Myc seedlings The products were immunoprecipated by anti-Myc agarose beads and detected by anti-streptavidin-HRP antibody. EGR2-Myc detected by anti-Myc antibody was used as a loading control.
Figure 2. Overview of lysine-acetylated proteins in the Calvin-Benson Cycle (B) identified in this study in Arabidopsis. The classification of proteins into functional bins was performed using MapMan (Thimm et al, 2004). Color code: proteins not identified in the LC-MS/MS analyses (white), proteins without identified lysine-acetylated sites (grey), and proteins with one (yellow), two (orange), three (dark orange), or four or more acetylation sites (red). For the Calvin-Benson cycle, each box indicates a separate Arabidopsis AGI identifier as indicated in Dataset EV6. Cytb6f, Cytochrome b6f; FBPase, Fructose-1,6-Bisphosphatase; GAPDH, Gyceraldehyde-3-phosphate dehydrogenase; PGK, Phosphogycerate Kinase; PPE, Phosphopentose epimerase; PPI, Phosphopentose isomerase; PRK. Phosphoribulokinase, PSII, Photosystem II; PSI, Photosystem I; RuBisCO, Ribulose-1,5-Bisphosphate-Carboxylase/Oxygenase; SBPAse, Seduheptulose-1,7-Bisphosphatase; TPI, Triose Phosphate isomerase; TK, Transketolase. A template of the light-reaction schematic was kindly provided by Jon Nield and modified.
B: Workflow for the predictive analysis combining the urine fragment size features via LR, RF, SVM and GLMEN models. sWGS data from 40 urine samples from patients with gliomas and 53 urine samples from controls were split into 5 subsets for training/validation (80% of the samples) and testing (20% of the samples), according to a 5 fold cross-validation approach and 50 random iterations (see Methods).
Western blotting for RPTOR, RICTOR and the indicated pluripotency proteins as in (A) (left) and quantification (right) (mean values ± SD). n = 3 biological replicates.
serum cholesterol (CHOL) levels in 9-week-old mice after 6 hr fasting (n=6-10 per group).
Scatter plot, analysis of VPS4B mRNA expression (number of transcripts per million) plotted against VPS4B copy number from TCGA CRC patient samples (n=376); plot generated using cBioPortal Gao et al, 2013(). Pie chart, summary of all types of VPS4B copy number alterations based on the analysis of data from 615 CRC samples deposited in the Colorectal Adenocarcinoma (TCGA, Provisional)
(b) Comparison of the amino acid sequences for SUMO1/2 and 3 and the yeast variant Smt3. Lysine residues are marked in blue. SUMO consensus motifs are marked in red. SUMO2/3 and Smt3 display a conserved consensus motif, whereas SUMO1 shows an inverted consensus motif.
D) LV-mediated delivery of RGS9-2 rescues the physiological response to quinpirole in Tor1a+/- slices. Dot plot reporting firing frequency changes induced by quinpirole in Tor1a+/+ (N=3) and Tor1a+/- (N=6) striatal ChIs infected with LV/HSV-RGS9-2 or control LV-GFP and HSV-LacZ ChIs (Tor1a+/+ RGS9-2 n=7; Tor1a+/- RGS9-2 n=9; Tor1a+/- LacZ/GFP n=8; Tor1a+/-: LacZ/GFP vs. RGS9-2 t test *P=0.0136)
(F) Quantitative analysis of cell and nuclear sizes in Fig 1E; n=5. Bars indicate mean±s.d. DmATG1, Drosophila Autophagy‐specific gene 1; dTOR, Drosophila TOR; qRT-PCR, quantitative real‐time reverse transcriptase-PCR.
C. Reference-free class averages and back-projections of the holoElongator model. Scale bar, 20 nm.
B) Heatmap showing TFs differentially expressed in Lo-G and Hi-G PDAC cell lines. Differentially expressed genes were identified based on a log2(fold change)> + 1 and an FDR < 0.1. Hierarchical clustering was based on Pearson correlation coefficients.
Statistical analysis of Pc and stx double mutant stxd77;; pcXT109 sleep profile compared with controls. total sleep (From left to right mean±SEM: 378.6±12.25, n=77; 455.0±11.17, n=80; 286.6±13.68, n=41; 356.7±14.50, n=43; 468.7±10.22, n=77; 467.2±13.49, n=80; 498.5±11.91, n=41; 500.9±17.84, n=43).
Scatter plot graph with the percentage of lung cancer-specific systemic CD4 T cells quantified by an autologous DC-based antigen presentation assay (See Materials and Methods), in a sample of G1 and G2 patients as indicated. Objective responses (OR) are shown in green. In red, patients with no OR.
Collective survival. A population survives only when its initial density is >NCT.
(A) Western blots of 6 months old quadriceps of WTFlx, KO and KO-NDUFS3 injected mice at 15-18 days old. GFP expression was observed only in the KO-GFP mice. Protein loading was obtained with stain-free gel technology. (B) Quantification of NDUFS3, COXI and SDHA expression levels in female mice showed in A.
c, TNF-α-mediated processing of humanATG16L1 in monocyte-derived macrophages. Scatter plot of immunoblot densitometry depicts a ratio of cleaved:full-length ATG16L1 (Extended Data Fig. 2). n = 7 donors.
(a) Isolated acini from wild-type (black) and Trpml1-/- mice (red) loaded with Fura2 were used to determine [Ca2+]i in response to stimulation with 100 µM carbachol. The traces are the mean±s.e.m. of 6 acinar clusters. Similar results were obtained in 3 experiments.
Chaining indices of the indicated genotypes. Statistical significance was compared with relative control. ; n = 8, 8, 7 (C)
D. Top three rows: Parasites expressing Cb EmeraldFP along with myoA-SNAP before and after Ca2+-Ionophore (A23187) or BIPPO treatment. The parasites were also treated with 2 µM of Cytochalasin D or DMSO (as control) for 30 min. After addition of A23187, preferential relocalisation of actin can be observed at the apical tip, while addition of BIPPO caused F-actin accumulation at the basal end. 4th row: myoAKO parasites expressing Cb-EmeraldFP before and after treatment with A23187 and BIPPO. While no apical or basal accumulation of F-actin is observed, some peripheral location of F-actin occurs in presence of A23187 or BIPPO, preferentially in the apical half of the parasite. Bottom row: adf cKD parasites expressing Cb-EmeraldFP before and after treatment with Ca2+ Ionophore or BIPPO. No apparent change in F-actin localisation can be seen upon treatment. E. Skeletonization of movies shown in D. Before addition of A23187 or BIPPO, RH and myoA KO parasites behave similar with no accumulation of F-actin at the apical tip. After treatment with calcium ionophore A23187, preferential accumulation of F-actin at the apical tip can be observed for RH (red arrowhead), while BIPPO presented preferential accumulation in the basal end (red arrowhead). In myoA KO, some relocalisation of actin is observed at the periphery of the parasite (yellow arrowhead). In the case of adf cKD, no relocalisation occurs. The images represent a projection of frames for each timepoint to provide a better overview of the dynamics of F-actin.
E. Immunofluorescence for H3K9ac and Neurofilament 200 (NF200) shows upregulated H3K9ac in vivo in DRG neurons after 966. Scale bar, 50 µm.
A, B Following treatment with 10 μM metformin for 3 days, the levels of HSF1 binding to endogenous HSP promoters were quantitated by chromatin IP (mean ± SD, n = 3, one-way ANOVA, **P < 0.01, ***P < 0.001). Normal rabbit IgG served as the negative control (A). Following the same treatment, HSP mRNA levels were quantitated by qRT-PCR (mean ± SD, n = 3, Student's t-test, ***P < 0.001) (B).
A. 293T cells were transfected with poly(I:C) and cell lysates were prepared at the indicated times for co-immunoprecipitation analysis of endogenous HDAC6 and RIG-I. Actin was used as the loading control.
D Schematic model depicting the FER- and LRX-dependent cell wall sensing mechanism, which limits vacuolar expansion (WT) in late meristematic cells. In contrast, dysfunctional cell wall perception in lrx3 lrx4 lrx5, fer-4 and 35S::LRR4-cit triggers premature vacuolar expansion.
D The Na+/K+ ratios in roots of TS-21, TS-422, TS-577, and TS-670 after treatment with 150 mM NaCl for the indicated time (n = 3 biological repeats).
Gene dose-dependent reductions in PTPδ protein levels in whole brains of Ptprd+/- (HT) and Ptprd-/- (KO) mice relative to Ptprd+/+ (WT) mice (P21), shown by immunoblot analyses. Note that the C-terminal antibody also detects a non-specific band of similar size indicated by an asterisk, possibly either PTPσ or LAR.
Population doublings (PDs) determined in BJ cells cultured with and without folate for 28 days.
G. Membrane binding assays demonstrating the absence of prazosin binding sites in GIC membrane preparations. Positive control shows that prazosin binds to membrane preparations of yeast expressing 1-AR. PRZ: prazosin. CRZ: cirazoline.
C. Left, representative flow cytometric analysis for CD3, CD4 and CD8, showing near normal T cell development in 10-day-old Rb∆K7 mice. Right, average of CD3, CD4 and CD8 single- and double-positive cell populations in Rb∆K7 vs control thymocytes with no significant difference (n.s.) by student's t-test. Results are presented as mean ±SD, n=4 biological replicates).
the virus isolated from lungs of ACE2-HB-01 mice at 3 dpi was observed by electron microscope (d).
(G) Representative Carmine alum stained mammary gland whole mounts from Wt and Hpn-/- mice. The histogram depicts quantification of duct length normalized to duct length in Wt mammary glands.
g, Percentage of cilia with depicted DS length after 8h and 48h SS. Three biological replicates, n>150 cilia per sample and repetition. Data shown include mean ±s.d. and P values of are calculated by two-tailed unpaired student t-test.
C Schematic illustration of the experiment. C57Bl/6 mice (11 weeks old) received adenovirus (2x109 PFU/mouse) for each candidate via tail-vein. GFP adenovirus was used as a control. Seven days after adenovirus infection, mice were euthanized and inguinal (iWAT) and epididymal (eWAT) white fat were weighed. n=5 for all the candidates and control.
(A) AKT phosphorylation in CSCs from MCF-7 cells sorted by CD44. GAPDH protein levels were used as a loading control.
g, Real-time RT-PCR of erythropoietin (Epo) (n = 12). For all relevant panels, statistical significance of the data (mean ± s.e.m.) is: *P < 0.05; **P < 0.01; ***P < 0.001.
D) Violin graph representing the area of NeoAgg404 (filled in light orange) and NeoAgg409 (filled in light purple) at day 3 and day 6 post seeding with FTLD-TDP-A and C seeds. Full bar represent median values, dashed lines represent quartiles. Two-way ANOVA with post hoc Fisher's LSD test, day 3: FTLD-TDP-A vs FTLD-TDP-C NeoAggpS404 p<0.0001, FTLD-TDP-A vs FTLD-TDP-C NeoAggpS409 p=0.0342, FTLD-TDP-A NeoAggpS409 vs FTLD-TDP-C NeoAggpS404 p=0.0047, FTLD-TDP-C NeoAggpS404 vs NeoAggpS409 p<0.0001; Day 6: FTLD-TDP-A vs FTLD-TDP-C NeoAggpS404 p<0.0001, FTLD-TDP-A NeoAggpS409 vs FTLD-TDP-C NeoAggpS404 p<0.0001, FTLD-TDP-A NeoAggpS404 vs FTLD-TDP-C NeoAggpS409 p<0.0001, FTLD-TDP-A NeoAggpS404 vs NeoAggpS409 p<0.0001, FTLD-TDP-C NeoAggpS404 vs NeoAggpS409 p<0.0001.
Cyclin E-expressing BJ cells were grown for 7 days with and without folate.Fork rate (Kb/min) distribution. White bars: BJ cells expressing an empty vector (n = 145); dark gray bars: BJ cells expressing the cyclin E oncogene (n = 147); light gray bars: BJ cells cultured for 7 days in a folate-free medium (n = 135); black bars: BJ cells expressing the cyclin E oncogene cultured for 7 days in a folate-free medium (n = 138).
(D) Immunoprecipitation analysis of K27 ubiquitination of MAVS in HEK293T cells infected with VSV for the indicated times. Anti-MAVS immunoprecipitates were analyzed using immunoblotting with an anti-MAVS or anti-Ub-K27 antibody. The WCL was immunoblotted with an anti-MAVS or anti-Ub-K27 antibody. Cell-based studies were performed independently at least three times with comparable results.
D NFR-mediated eEF2 phosphorylation is not affected in AMPKα1α2 dKO. AMPKα1α2 WT and dKO MEF treated for 6h with indicated concentration of NFR, Rapamycin (Rapa), AICAR or starved for 1h in PBS (Starv.), were analyzed by immuoblot with indicated antibodies. * anti-total AMPKα antibody give an unspecific band with a slightly higher molecular weight in dKO cells. Tubulin is used as loading control. Each panel is representative of at least 3 independent experiments.
(C) In vitro fusion assay of ATG16L1 and mATG9 vesicles. HeLa cells were transfected with control or VAMP3 siRNA for 4 days separately and, on the fourth day, either transfected with mStrawberry-ATG16L1 or ATG9L1-pEGFP. PNS of cells expressing the two constructs were mixed for 1 hr at 37C° and observed by confocal microscopy. Histogram shows the correlation of vesicles carrying ATG16L1 that fuse with vesicles carrying mATG9 (Pearson's coefficient). Error bar, SEM. ∗∗∗p < 0.001. The total number of structures per field (ATG16L1 and ATG9L1) is not significantly different in the control and KD samples (control, 37 ± 3; VAMP3 KD, 45 ± 3; ± SEM; p = 0.106).
D. MA plot comparing gene expression between si6B-NT and NT-2. The data analyzed derive from two independent biological replicates. Arrows represent data points outside of the plotting area. DEG, differentially expressed genes.
(F) LN229 cells transfected with a pool of four siRNAs targeting NEDD4L (+) or a scrambled siRNA (-) were detached (Susp., suspension) by trypsinization using the procedure described in Methods and reseeded (Attach, attached) for two hours. Total lysates from these cells were subjected to western blotting.
E. Quantification of nuclear and mitochondrial transcript levels in control and patient fibroblasts. The results from control and patient cells were averaged, normalized to controls and displayed as mean ± SEM (n=3 biological replicates for each condition).
H, Enrichment of commonly affected transcripts between Ctrl/S vs Ctrl/SD and Ctrl/SD vs Fxr1/SD comparisons in GO:BP.
(F) Co‐immunoprecipitation experiments using lysates from HEK293 cells transiently expressing YFP-LC3 with β‐cateninWT or β‐cateninW504A/I507A starved for 8 h with chloroquine (10 μM). YFP-LC3 immunoprecipitated β‐cateninWT but not β‐cateninW504A/I507A. Input lysates are shown and immunoprecipitated p62 and YFP-LC3 served as positive controls.
(A) Images of mock-infected high-pressure-frozen and freeze-substituted Huh7.5 cells. (B-E) Micrographs of high pressure frozen freeze-substituted Huh7.5 cells harvested 16, 24, 36 and 48 h after infection with Jc1 (100 TCID50/cell). Yellow squares indicate the areas that are shown at higher magnification on the right of each subpanel. Note the time-dependent increase of complexity of HCV-induced membrane alterations. ER, endoplasmic reticulum; m, mitochondria; MVB, multi-vesicular bodies; LD, lipid droplet; DMV, double membrane vesicle; MMV, multi membrane vesicle; DMT, double membrane tubule (labeled with a black arrow).
F Schematic view of the non-canonical Activin A pathway, including inhibitors used for the experiments described in this figure.
L) Relative parasite growth was measured in scrambled siRNA transfected HFFs and TRAF6 knockdown HFFs using luciferase-based assay with indicated strains with and without IFNγ (n=3). The antibody against TRAF6 used here was from Abcam (Appendix Table S2). Full length blots are in supplementary figures 4l-m.
ChIP-qPCR detection of Ddx17 (H) and PCAF (I) occupancies at the myogenin locus in Myoparr-depleted differentiating C2C12 cells. The data were normalized to input values. n = 3, mean ± SD. *p < 0.05 (unpaired two-tailed Student's t-test).
(E) Titration of Pol δ in a pulse chase reaction on the Tg template. Pol δ was added after the 0 min time point was taken.
a, Cherry::APE1GFP::ATG8 cells were grown and shifted to YPD, nitrogen starvation (SD-N) or YPD+VPA medium for 3 h. Samples were processed for microscopy. The table shows numbers corresponding to the experiment. Percentage of fluorescence signals is presented and error bars represent the s.d. obtained from three independent experiments. DIC, differential interference contrast. Scale bars, 3 µm.
(F) Total cell lysates from cells grown in normal or starvation media for 6 hr were subjected to immunoblotting. LC3-I and LC3-II denote cytosolic and lipidated LC3B, respectively.
E. Soft agar colony formation assay of parental and LCS-KO HeLa cells expressing LCS-HA or GOLPH3. Colony number was quantified; (individual data points are shown in the graphs; data are means ± SD of at least 3 independent experiments; ***p <0.001 [Student's t-test]).
IFA of ISP1 and ISP3 expression from zygote to ookinete of the isp1::6HA and isp3::6HA parasites, respectively. Scale bar = 5 μm.
Quantification of the number of germband macrophages in embryos from control and upon RNAi knockdown of the genes In (G) сontrol 1 (n=18 embryos) vs. LKR/SDH RNAi 1 (n=17) and control 2 (n=21) vs. LKR/SDH RNAi 2 (n=23), both p<0.0001. Data information: Mean±SEM and ns=p>0.05, ****p<0.0001. One-way ANOVA with Tukey
D. Pathology of wild type and RKIP deficient chimeric mice in response to VSV infection. Hematoxylin and eosin staining of liver and lung sections from mice in (A). Scale bar, 100 μm (left) or 50 μm (right).
(A) Quantification of cumulative mitotic entry over time immediately following (1μM) palbociclib treatment in a range of tumour cell lines, as indicated. Mitotic entry of 50 cells per condition were analysed immediately following drug addition. Graph shows cumulative mean -/+ SD from three experiments.
In silico assessment of the mitochondrial proteome ('MitoCarta 2.0', (Calvo et al., 2016)). The MitoCarta was searched for the following DPP9 recognition motifs: MPX (X not P) or MYPX (Y not D, X not P). More than 100 MitoCarta proteins fulfil this criterion. Of those, many do not carry an N-terminal mitochondrial targeting sequence (MTS) but instead internal or C-terminal targeting signals. Proteins found in IMS and IMM appear to be enriched.
I The viability of Jurkat T cells was assessed after incubation with 1 µM CXCL12 alone and in the presence of Gal-3, Gal-3 CRD and Gal-1 (n = 2).
(C and D) RNAi of CG34015 in the wing P-compartment significantly reduces the size in both sexes. The images are taken from female wings. Statistical significance was evaluated by Wilcoxon test.
HT-29 cells were treated as indicated. Cell lysates were analyzed by Western blotting (J). Data information: Cleaved MLKL is indicated with an arrowhead.
TUNEL assays on eye and brain sections of stage (st) 30 medakafish control and MO-injected embryos reveal a decrease in the number of apoptotic cells in both hccs-MO/miR-181a/b-MO- and cox7b-MO/miR-181a/b-MO-injected compared to hccs-MO- and cox7b-MO-injected embryos. Scale bars are 20μm.
E. Transcript levels of DAM gene transcripts significantly altered in GRN-/- hiMGL, treated with Ab1 or Ab2 in comparison to isotype treatment from the data set in A, normalized to the mean of the WT hiMGL samples (n=4, biological replicates). Data information: Data represent mean ± SEM. For statistical analysis one-way ANOVA with Dunnett`s post hoc test was used to compare Ab1 and Ab2 (20 µg/ml and 40µg/ml) conditions to the isotype treated condition, Statistical significance was set at *, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001, *****, p < 0.00001, and ns, not significant.
A) Schematic illustration of the subcellular fractionation of mESCs. Fractions S1, S2, and P are mainly composed of cytosolic proteins, nuclear soluble proteins, and chromatin-bound proteins, respectively.
(d) Co-IP experiments to assess the competition between Beclin 1-Atg14L and Beclin 1-UVRAG complexes in vivo. FLAG- or GFP-tagged Atg14L or UVRAG was transfected alone, or both were co-transfected into HEK 293T cells and their interaction with endogenous Beclin 1 was probed by co-IP using GFP antibody, followed by western blot with anti-Beclin 1 antibody.
C Cytokine levels in the plasma of COVID-19 patients with (n=12) or without (n=6) GI symptoms by cytokine assay. Data information: All data are shown as mean ± SD. P values are determined by Student's t-test.
A. HeLa cells transfected with siCtrl or siBclaf1-1 were treated with TNF (10 ng/ml) for the indicated time periods followed by western blotting analysis with the indicated antibodies.
FAmplitude of 1st ERK activity peak in response to different sustained or pulsed GF stimulation experiments. Notched boxplots of 1st ERK activity peak amplitude with median, interquartile range (box), and data within 1.5 IQR range of the lower and upper quartiles (whiskers) are shown (n = 20 cells per experiment).
C. GOLPH3 OE or KD HeLa cells expressing LCS-HA were fixed and processed for IF: HA (green). Asterisks indicate GOLPH3 OE cells. Scale bar, 20 µm.