OPCML abrogates Gas6/AXL mediated increase in cell motility and invasion.Figure 4 Single cell tracking analysis upon stimulation with Gas6 over 12 hour of Empty "E" or OPCML "O" (A) SKOV3 cell  Single cell tracking analysis upon stimulation with Gas6 over 12 hour of Empty "E" or OPCML "O" (B) PEA1 cells Data in (A) to (N) are representative of at least three experiments with graphs depicting means ± SEM: *P < 0.05, **P < 0.01, and ***P < 0.001 by Student"s t tests Gap closure assay and analysis of Empty "E" or OPCML "O" SKOV3 (C,D s, dashed white lines represent initial boundary and scale bars = 200μm Gap closure assay and analysis of Empty "E" or OPCML "O" SKOV3 (C,D s, dashed white lines represent initial boundary and scale bars = 200μm Data in (A) to (N) are representative of at least three experiments with graphs depicting means ± SEM: *P < 0.05, **P < 0.01, and ***P < 0.001 by Student"s t tests Gap closure assay and analysis of Empty "E" o PEA1 (E,F) cells, dashed white lines represent initial boundary and scale bars = 200μm Gap closure assay and analysis of Empty "E" o PEA1 (E,F) cells, dashed white lines represent initial boundary and scale bars = 200μm Data in (A) to (N) are representative of at least three experiments with graphs depicting means ± SEM: *P < 0.05, **P < 0.01, and ***P < 0.001 by Student"s t tests (G) Spheroid invasion assay of Empty "E" or OPCML "O" SKOV3 spheroids 72 hours post stimulation with Gas6. Scale bars = 1mm (H) Invasion from (G) was quantified and normalised to spheroid volume prior to the addition of chemotactic medium Data in (A) to (N) are representative of at least three experiments with graphs depicting means ± SEM: *P < 0.05, **P < 0.01, and ***P < 0.001 by Student"s t tests (I) Western blotting of fallopian tube epithelium cells FT190 transfected with non-targeting siRNA (NTrg) or two siRNA for OPCML (si 6 and si 7). GAPDH was used as loading control (J) Analysis of Gas6-stimulated single cell motility of FT190 cells transfected with siRNA for OPCML compared to Non-Targeting siRNA control Data in (A) to (N) are representative of at least three experiments with graphs depicting means ± SEM: *P < 0.05, **P < 0.01, and ***P < 0.001 by Student"s t tests (K) Western blotting of ovarian cancer cell lines PEO1, OVCA429, SKOV3, HEYA8 expressing different levels of AXL, and transduced with Empty vector control "E", or OPCML "O" ovarian cancer cell lines PEO1, OVCA429, SKOV3, HEYA8 expressing different levels of AXL, and transduced with Empty vector control "E", or OPCML "O", and (L) Densitometry Data in (A) to (N) are representative of at least three experiments with graphs depicting means ± SEM: *P < 0.05, **P < 0.01, and ***P < 0.001 by Student"s t tests (M, N) Quantitation of gap closure assays of ovarian cancer cell lines PEO1, OVCA429, SKOV3, HEYA8 transduced with OPCML "O", or Empty vector control "E"; dashed white lines represent initial boundary and scale bars = 200μm. Data in (A) to (N) are representative of at least three experiments with graphs depicting means ± SEM: *P < 0.05, **P < 0.01, and ***P < 0.001 by Student"s t tests (M, N) Quantitation of gap closure assays of ovarian cancer cell lines PEO1, OVCA429, SKOV3, HEYA8 transduced with OPCML "O", or Empty vector control "E"; dashed white lines represent initial boundary and scale bars = 200μm