Figure 5A. Top: schematic representation of wild-type (wt) human BRD9 and selected mutants, highlighting the acyl-binding bromodomain (BD9) and the DUF3512 domain responsible for ncBAF assembly. Numbers represent amino-acid residues. The box highlights BD9 positions 208-216, which include residues critical for acetyl (N216) or butyryl (M208) binding. dBD is an engineered BRD9 variant lacking the entire bromodomain (residues 140-237). Bottom: western blot analysis of CTRL#3 or BRD9-KO#3 cells stably transduced with lentiviruses expressing empty vector (EV), or wt and mutant BRD9 constructs. B. The various CTRL#3 or BRD9-KO#3 cells described in (A) were stimulated with 1000 IU/mL of IFN-α2 for 16h prior to infection with VSV-GFP at an MOI of 0.6 PFU/cell. Total Integrated Green Fluorescent Intensities were determined using the Incucyte live-cell analysis system at 48h post-infection. Data information: data represent means and standard deviations from n=3 biological experiments (individual data points shown). Numbers above IFN-α2-treated bars indicate their approximate difference to the respective untreated conditions. Dotted lines are a visual guide for minimum virus replication in control cells in the presence of IFN-α2. Statistical significance was determined by 1-way ANOVA on log-transformed intensity values (***p-value < 0.001; ****p-value < 0.0001; n.s. not significant). C. Schematic representation of HA-tagged wt human BRD9, BRD7, and selected chimeric mutants. BD7 is the BRD7 bromodomain. Numbers represent amino-acid residues of the final constructs. D. Western blot analysis of CTRL#3 or BRD9-KO#3 cells stably transduced with lentiviruses expressing empty vector (EV), or HA-tagged wt and chimeric mutant constructs described in (C). E. The various CTRL#3 or BRD9-KO#3 cells described in (D) were stimulated and infected as described in (B). Data information: , data represent means and standard deviations from n=3 biological experiments (individual data points shown). Numbers above IFN-α2-treated bars indicate their approximate difference to the respective untreated conditions. Dotted lines are a visual guide for minimum virus replication in control cells in the presence of IFN-α2. Statistical significance was determined by 1-way ANOVA on log-transformed intensity values (***p-value < 0.001; ****p-value < 0.0001; n.s. not significant). F. Schematic representation of wt human BRD9, and selected chimeric mutants with domains from BRD7. Numbers represent amino-acid residues of the final constructs. G. Western blot analysis of CTRL#3 or BRD9-KO#3 cells stably transduced with lentiviruses expressing empty vector (EV), or wt and chimeric constructs H. Western blot analysis of input lysates and anti-BRD9 immunoprecipitations (IPs) from cells described in (G) expressing wt BRD9 or the chimeric BRD7DUF. The indicated proteins were detected with specific antibodies. Data are representative of two biological replicates. I. The various CTRL#3 or BRD9-KO#3 cells described in (G) were stimulated and infected as described in (B). Data information: data represent means and standard deviations from n=3 biological experiments (individual data points shown). Numbers above IFN-α2-treated bars indicate their approximate difference to the respective untreated conditions. Dotted lines are a visual guide for minimum virus replication in control cells in the presence of IFN-α2. Statistical significance was determined by 1-way ANOVA on log-transformed intensity values (***p-value < 0.001; ****p-value < 0.0001; n.s. not significant).