EMBO/sd-character-level-ner huggingface dataset:
Transcriptomics profiles during PGC‐precursor and PGCLC inductionUnsupervised hierarchical clustering of HuES6 ESCs, 383.Figure 5Unsupervised hierarchical clustering of HuES6 ESCs, 383.2 iPSCs, d2 PGC‐precursor cultures, and FACS‐sorted PGCLCs. Principal component analysis of HuES6 ESCs, 393.2 iPSCs, d2 PGC‐precursor cultures, and FACS‐sorted PGCLCs. Scatter plots of global gene expression microarrays comparing d2 PGC‐precursor cultures, or d4 or d6 FACS‐sorted PGCLCs with iPSCs. Venn diagrams of the transcriptomics meta‐analysis with the intersections of the DEGs of mouse in vitro PGCLCs, human in vivo PGCs, and human in vitro PGC‐like cells in relation to their respective pluripotent counterparts in their respective platforms. Heatmap of pluripotent, germ cell, mesodermal, and chromatin‐related markers in mouse (left) and human (right) transcriptomics samples (Tfap2c is not targeted in the Illumina MouseRef‐8 v2). The color bars codify the gene expression in log2 scale. Red corresponds to high gene expression. Blimp1+/Stella+ PGCLCs are denoted "B+S+", and Blimp1+/Stella− PGCLCs are denoted "B+S−". Effect of knockdown of BLIMP1 and PRDM14 on PGCLC induction. Knockdown of BLIMP1 (F) and PRDM14 (G) was done using the lentiviral system with two individual shRNA vectors for each gene. The knockdown efficiencies were assessed by qPCR (left panel, each). The induction of d4 PGCLCs analyzed by FACS, gated for TRA‐1‐81 and cKIT (right panel, each). Data are presented as means ± SD (n = 3).
Sen1 is required for efficient RNAP3 transcription termination.Figure 4Snapshots of ChIP-seq signals of the RNAP3 subunits Rpc1 and Rpc2 in the presence or absence of Sen1 across a representative (A) tRNA gene, (B) 5S rRNA gene and (C) srp7. Boxed regions highlight the increased density of reads in the downstream region of genes in the absence of Sen1. Average ChIP-seq profile of Rpc1 and Rpc2 across all isolated tRNA and 5S rRNA genes in the presence and absence of Sen1. Scanning of Rpc37-3flag occupancy at three different tDNA loci in the absence of Sen1 by ChIP-qPCR (mean ± standard deviation from 6 biological replicates).