Figure 6A Representative images of lungs and spleens from WT and Akt2 KO mice treated with TMPD (500μL per mouse) for 2 weeks, and the corresponding H&E-staining of the tissues. Bar, 200 μm. Data information: * Data are representative of three independent experiments qRT-PCR analysis of the relative Ifnb1 and Ifna4 mRNA expression levels in PICs (n≥5) and PBMCs (n≥4) (B) from WT and Akt2 KO mice Data information: *P < 0.05, **P < 0.01 and ****P < 0.0001; using unpaired t-test Data are representative of three independent experiments Error bars , mean ± SD). immunoblot analysis of IRF3, LaminB1 and GAPDH in the PIC nuclear and cytoplasmic fractions (C) from WT and Akt2 KO mice treated with TMPD for 2 weeks. GAPDH and LaminB1 were used as cytoplasmic and nucleic protein loading control respectively. Data information: * Data are representative of three independent experiments Representative images of lipogranulomas in peritoneal cavity in the kidney sections were collected from WT and Akt2 KO bone marrow-reconstituted CD45.1 mice 12 weeks after TMPD treatment. Bar, 50 μm. Data information: * Data are representative of three independent experiments H&E-staining (E left panel) and immunofluorescence of IgG deposits (E right panel) in the kidney sections were collected from WT and Akt2 KO bone marrow-reconstituted CD45.1 mice Bar, 50 μm. Data information: * Data are representative of three independent experiments Quantitative analysis of serum anti-dsDNA IgG antibody by ELISA (F, n≥4) from WT and Akt2 KO bone marrow-reconstituted CD45.1 mice 12 weeks after TMPD treatment. Data information: *P < 0.05, **P < 0.01 and ****P < 0.0001; using two-way ANOVA test Data are representative of three independent experiments Error bars mean ± SD). mRNA expression levels of PIC Ifnb1 and Ifna4 by qRT-PCR (G, n≥4) from WT and Akt2 KO bone marrow-reconstituted CD45.1 mice Data information: *P < 0.05, **P < 0.01 and ****P < 0.0001; using unpaired t-test Data are representative of three independent experiments Error bars mean ± SD). FACS analysis of the percentages of B220+ and CD19+MHCⅡ+ cells in PBMC (H, n≥4) from WT and Akt2 KO bone marrow-reconstituted CD45.1 mice Data information: *P < 0.05, **P < 0.01 and ****P < 0.0001; using unpaired t-test Data are representative of three independent experiments Error bars mean ± SD). I qRT-PCR analysis of the relative mRNA expression levels of AKT2 in PBMC isolated from SLE patients (n=9) or healthy donors (n=9). Data information: *P < 0.05, **P < 0.01 and ****P < 0.0001; using unpaired t-test Data are pooled from different individuals Error bars mean ± SD). J. qRT-PCR analysis of the relative mRNA expression levels of AKT2 (left panel) and IFNA (middle panel) or IFNβ1 (right panel) in CD14 positive monocytes isolated from SLE patients (n=9) or healthy donors (n=9). Data information: *P < 0.05, **P < 0.01 and ****P < 0.0001; using unpaired t-test or correlation analyses Data are pooled from different individuals Error bars mean ± SD). K Immunofluorescence of IRF3 (Red), AKT2 (Green), DAPI (Blue, nucleus) in CD14+ cells from SLE patients followed by analysis of the cells about mean amount of AKT2 in this cell as well as mean amount of IRF3 in the same nucleus with Image J software. Cells (n=39) were collected 3 patients. Bar, 2 μm. Data information: *P < 0.05, **P < 0.01 and ****P < 0.0001; using correlation analyses Data are pooled from different individuals or representative of three independent experiments