Figure 5A FACS analysis of the percentage of macrophages (CD11b+ F4/80+ cells) in WT and Akt2 KO BM and spleen. Data information: * Data are representative of three independent experiments respectively B Survival rates of WT and Akt2 KO mice after lethal dose injection of VSV during 120 hours (1 × 107 PFU/g, i.v.). Data information: *P < 0.05, **P < 0.01; using a log-rank (Mantel-Cox) test Data are representative of three independent experiments respectively C qRT-PCR analysis of the Ifnb1 mRNA expression in the liver (n=8), spleen (n=7) and lung (n=7) of WT or Akt2 KO mice 6 hours after the injection of VSV (1 × 106 PFU/g, i.v.). Data information: *P < 0.05, **P < 0.01; using a paired t-test Data are representative of three independent experiments respectively , in which the different groups of mice were sacrificed and quantified for indicated experimental purposes D IFNβ1 concentrations from WT (n=8) and Akt2 KO (n=7) serum were detected by ELISA 3 hours after VSV infection (1 × 106 PFU/g, i.v.). Data information: *P < 0.05, **P < 0.01; using a unpaired t-test Data are representative of three independent experiments respectively in which the different groups of mice were sacrificed and quantified for indicated experimental purposes Error bars mean ± SD). E WT and Akt2 KO mice were injected with VSV for 24 hours (1 × 106 PFU/g, i.v.). Representative images of the VSV infected cells (GFP) were collected by fluorescence microscope (left panel) and the VSV copies (WT, n=6; Akt2 KO n=7) were measured by qRT-PCR (right panel) in the liver of mice. Bar, 100 μm. Data information: *P < 0.05, **P < 0.01; using a unpaired t-test Data are representative of three independent experiments respectively in which the different groups of mice were sacrificed and quantified for indicated experimental purposes Error bars mean ± SD). Bone marrow cells from WT and Akt2 KO mice (CD45.2) were adoptively transferred to lethally irradiated CD45.1 mice and infected with VSV. Survival rates (F left panel), serum IFNβ1 concentrations (F right panel, n=5) were analyzed Bar, 100 μm. Data information: *P < 0.05, **P < 0.01; using a paired t-test or log-rank (Mantel-Cox) test Data are representative of three independent experiments respectively in which the different groups of mice were sacrificed and quantified for indicated experimental purposes Bone marrow cells from WT and Akt2 KO mice (CD45.2) were adoptively transferred to lethally irradiated CD45.1 mice and infected with VSV. VSV copies (G, n=9) were analyzed Data information: *P < 0.05, **P < 0.01; using a unpaired t-test Data are representative of three independent experiments respectively , in which the different groups of mice were sacrificed and quantified for indicated experimental purposes Error bars , mean ± SD). Bone marrow cells from WT and Akt2 KO mice (CD45.2) were adoptively transferred to lethally irradiated CD45.1 mice and infected with VSV. representative images were analyzed Data information: * Data are representative of three independent experiments respectively I Survival rates of WT mice that were intraperitoneal injected with CCT128930 (20 mg/kg) twice every other day before VSV infection (1 × 107 PFU/g, i.v.). Data information: *P < 0.05, **P < 0.01; using a log-rank (Mantel-Cox) test Data are representative of three independent experiments respectively