Figure 1(A) Structural formula of 144DG11. (B) Kaplan-Meier survival curve based on n=17 144DG11-treated and n=9 vehicle-treated animals. 144DG11 significantly increased survival (log-rank test p-value<0.000692). (C) Weight, in movement in an open field as a function of time after treating Gbeys/ys mice with vehicle (n=8), or 144DG11 (n=9). Data information: Two way ANOVA with repeated measures show that, throughout the period, treatment values were higher than vehicle in (p<0.05) Significant difference (p<0.05, two-tailed t-tests) at specific time points is denoted by *. All error bars represent s.d. (D) average duration in movement in an open field as a function of time after treating Gbeys/ys mice with vehicle (n=8), or 144DG11 (n=9). Data information: Two way ANOVA with repeated measures show that, throughout the period, treatment values were higher than vehicle (p<0.1) Significant difference (p<0.05, two-tailed t-tests) at specific time points is denoted by *. All error bars represent s.d. (E) Upper panel, time spent at location (average based on n=9 nine month old mice) is quantified as a heat map (blue (low) to red (high) scale). Lower panel, visual tracking examples from single animals. wt, vehicle-treated wt animals; tg treated, 144DG11-treated Gbeys/ys mice; tg, vehicle-treated Gbeys/ys mice. (F) Gait analysis of n=9 nine month old mice treated with vehicle or 144DG11. Shown are average (+/- s.d.) stride lengths. A representative ink foot-print trail is also shown for each arm. Statistically significant differences (One Way ANOVA with Sidak's post-hoc tests) were demonstrated between 144DG11 and vehicle treated transgenic mice, and between transgenic and wt mice. (G Extension reflex as a function of time after treating Gbeys/ys mice with vehicle (n=8), or 144DG11 (n=9) or wt mice with vehicle (n=8). Data information: Two way ANOVA with repeated measures show that, throughout the period, treatment values were higher than vehicle (p<0.05) Significant difference (p<0.05, two-tailed t-tests) at specific time points is denoted by *. All error bars represent s.d. H) Extension reflex as a function of time in another experiment Gbeys/ys with vehicle (n=8), or 144DG11 (n=9), or wt mice with vehicle (n=8). Data information: Two way ANOVA with repeated measures show that, throughout the period, treatment values were higher than vehicle (p<0.05) Significant difference (p<0.05, two-tailed t-tests) at specific time points is denoted by *. All error bars represent s.d. I, J (I) (vehicle) and (J) (144DG11) show 7 month old Gbeys/ys mice following 3 months under the respective treatments. Note unkempt fur in vehicle treated animal, which was improved by 144DG11. (K) Experimental design. (L) weight as a function of time after treating Gbeys/ys mice with vehicle (n=8) or 144DG11 (n=9) at 6 months of age (at onset). Data information: Two way ANOVA with repeated measures show that, throughout the period, treatment values were higher than vehicle (p<0.15). (M) average duration in movement in an open field as a function of time after treating Gbeys/ys mice with vehicle (n=8) or 144DG11 (n=9) at 6 months of age (at onset). Data information: Two way ANOVA with repeated measures show that, throughout the period, treatment values were not different than vehicle (p<0.15). (N extension reflex as a function of time after treating Gbeys/ys mice with vehicle (n=8) or 144DG11 (n=9) at 6 months of age (at onset). Data information: Two way ANOVA with repeated measures show that, throughout the period, treatment values were higher than vehicle (p<0.07) Significant difference (p<0.05, two-tailed t-tests) at specific time points is denoted by *. All error bars represent s.d.