Figure 2(A) Heat map showing RPKM (Reads Per Kilobase per million of mapped reads) mean values of Mmps from mouse peritoneal macrophages stimulated with AG (1 μg/ml) for 24 h or left untreated (NT). (B) Quantitative polymerase chain reaction (qPCR) analysis of Mmps including Mmp2, Mmp9, Mmp10, Mmp12 and Mmp13 mRNA from mouse peritoneal macrophages stimulated with AG (1 μg/ml) for indicated times. (C) Immunoblots of cell supernatants to analyze secreted MMP2, MMP9, MMP10, MMP12 and MMP13 by mouse peritoneal macrophages stimulated with AG (1 μg/mL) for indicated times; GADPH of cell lysates served as a loading control. Data information: Data are means + SD averaged from 3 independent experiments performed with technical triplicates and each symbol represents the mean of technical triplicates. Two-way ANOVA followed by Tukey's post hoc test was used for statistical analysis. *, p<0.05; **, p<0.01; ***, p<0.001; ****, p<0.0001. (D) qPCR analysis of Mmps including Mmp2, Mmp9, Mmp10, Mmp12, and Mmp13 from the lungs of mice at indicated days post intraperitoneal administration of AG (100 μg). Data information: Data are means + SD of indicated numbers of mice from 1 of at least n=2 independent experiments and each symbol represents data from 1 mouse. Data shown are representative of n=2 independent experiments. Two-way ANOVA followed by Tukey's post hoc test was used for statistical analysis. *, p<0.05; **, p<0.01; ***, p<0.001; ****, p<0.0001. (E) qPCR analysis of Mmps including Mmp9, Mmp10, Mmp12, and Mmp13 from mouse peritoneal macrophages stimulated with AG (1 μg/ml) for 24 h in absence or presence of AG aptamers (0.5 μg/ml). Data information: Data are means + SD averaged from 3 independent experiments performed with technical triplicates and each symbol represents the mean of technical triplicates. Two-way ANOVA followed by Tukey's post hoc test was used for statistical analysis. *, p<0.05; **, p<0.01; ***, p<0.001; ****, p<0.0001. (F) Immunoblots of cell supernatants to analyze secreted MMP9, MMP10, MMP12 and MMP13 by mouse peritoneal macrophages stimulated with AG (1 μg/ml) for indicated times in absence or presence of AG aptamers (0.5 μg/ml); GADPH of cell lysates served as the loading control. (G) qPCR analysis of Mmps including Mmp9, Mmp10, Mmp12, and Mmp13 from the lungs of mice at 3 days post intraperitoneal administration of AG (100 μg) in absence or presence of AG aptamers. AG aptamers (1 μg/mouse) were intranasally administrated per day. Data information: Data are means + SD of indicated numbers of mice from 1 of at least n=2 independent experiments and each symbol represents data from 1 mouse. Data shown are representative of n=3 independent experiments. Two-way ANOVA followed by Tukey's post hoc test was used for statistical analysis. *, p<0.05; **, p<0.01; ***, p<0.001; ****, p<0.0001. (H) qPCR analysis of Mmps including Mmp9, Mmp10, Mmp12, and Mmp13 from mouse peritoneal macrophages infected with H37Rv for 24h (MOI=5) in absence or presence of AG aptamers (1 μg/ml). (I) Immunoblots of cell supernatants to analyze secreted MMP9, MMP10, MMP12 and MMP13 by mouse peritoneal macrophages infected with H37Rv for indicated times (MOI=5) in the absence or presence of AG aptamers (0.5 μg/ml); GADPH of cell lysates served as the loading control. Data information: Data are means + SD averaged from 3 independent experiments performed with technical triplicates and each symbol represents the mean of technical triplicates. Two-way ANOVA followed by Tukey's post hoc test was used for statistical analysis. *, p<0.05; **, p<0.01; ***, p<0.001; ****, p<0.0001. (J) qPCR analysis of Mmps including Mmp9, Mmp10, Mmp12, and Mmp13 from lungs of mice intranasally infected with H37Rv (2×106 cfu/mouse) for 4 weeks in absence or presence of AG aptamers. AG aptamers (1 μg/mouse) were intranasally administrated once at a 1 week-interval. Data information: Data are means + SD of indicated numbers of mice from 1 of at least n=2 independent experiments and each symbol represents data from 1 mouse. Data shown are representative of n=3 independent experiments. Two-way ANOVA followed by Tukey's post hoc test was used for statistical analysis. *, p<0.05; **, p<0.01; ***, p<0.001; ****, p<0.0001.