EMBO/sd-character-level-ner huggingface dataset:
F) The BDNF-induced increase in cytoplasmic TRBP is dependent on intracellular calcium. Cortical neurons (7 DIV) were treated with vehicle control, BDNF and BAPTA-AM as indicated, and cytoplasmic fractions were isolated using digitonin. Protein intensity values were normalized to actin in each independent experiment (p=0.03, n=3, error bars; s.d.).
A Pie chart depicting fine-mapping results (Huang et al, 2017) (posterior probabilities) for an IBD-associated locus on 21q21 (left panel). MPRA results in resting (centre panel) and stimulated CD4 T cells (right panel) showing that the putative causal variant has significant expression-modulating effect.
B, Proteomic analysis highlighting proteins associated with the ER stress response significantly down-regulated in THEM6 KO 22rv1 cells when compared to CTL (p-value ≤ 0.05, FC = 1.3). Data information: Data are presented as mean values +/- SD. Data reproducibility: n = 3 independent biological experiments.
(D) (Top) Fnip1 protein expression in the gastrocnemius muscle from the indicated mice. (Bottom) Quantification of the Fnip1/Tubulin signal ratio (n = 4 mice per group). *p = 0.014.
Fig. 1. A co-culture assay based on the split TEV technique for monitoring NRG1-ERBB4 signaling activity.(B) Dose-response assay using increasing numbers of Nrg1 type I β1a-expressing PC12 cells. For each 96-well, 40,000 split TEV assay cells were co-plated with increasing numbers of Nrg1-expressing cells, and incubated for 24h. Half-maximal activation is reached at 5,000 Nrg1-expressing cells. The Z' factor is 0.5 indicating a large separation band for this assay.
(f) HL-1 cells were transfected with control, p53, p21 or p16-specific siRNAs, followed by treatment with 0.02 μM DOX and mitophagy induction. Mitochondrial content is assessed by flow cytometry from four independent experiments performed in duplicate.
D. Western blot on IGR37 and 39 cellular lysates probed with anti-HMB45 PMEL antibody and anti HDAC2 to check the loading of similar amount of total lysates.
Representative image of the anaphase bridge (indicated with arrows) in the basal crypt of G3Terc-/- mice (G). The percentage of cells with anaphase bridges in pH3 positive basal crypt cell population (n=4-6 mice per genotype) (H).
D. Prok2RA application resulted in a significant period lengthening effect of ongoing SCN ensemble Per2::Luciferase rhythms, which was reversed upon drug washout (p=0.0002, ***p=0.0009, *p=0.01) (n = 22 independent SCN slices with repeated measure).
Subcutaneous injection of Smaducin-6 increases the survival rate of severe CLP-induced sepsis mice (BALB/c mice). Different amounts of Smaducin-6 were subcutaneously injected at 2 h post-CLP followed by three injections at 12-h intervals. n = 10 mice per group per experiment.
A HEK293T cells were transfected with plasmids expressing HA-tagged SHP2 and GFP-tagged TLR7. After 48 h, cells were left unstimulated or stimulated by IMQ (10 µg/ml) for 30 min. Cell lysates were immunoprecipitated by anti-HA or anti-GFP and probed by indicated antibodies.
Left: Representative microphotographs of PAS staining from WT and SOD1G86R tibialis anterior cross-sections at 65 and 105 days of age showing glycogen-negative (light pink) and glycogen-positive (dark pink) fibers. Scale bar: 200 μm. Right: Quantification of glycogen-negative (−) and glycogen-positive (+) fibers at 65 and 105 days of age. For (−) fibers: **P-values versus WT: 0.0026 at 65 days and 0.0074 at 105 days, for (+) fibers: ##P-values versus WT: 0.0025 at 65 days and 0.0073 at 105 days (n = 5/genotype at 65 days and n = 4/genotype at 105 days, two-way ANOVA followed by Fisher's LSD post hoc test).
F. Salt-stable DNA association requires ATP binding and Smc5/6 head-engagement, but not ATP hydrolysis. Same experimental setup as in (D) using mutant versions of the Smc5/6 hexamer as well as the non-hydrolyzable analogue of ATP, ATPγS, as indicated.
(B) Knockout of HMGB1 limits the disassociation of the Bcl-2-Beclin1 complex during treatment with autophagic stimuli. HMGB1−/− and HMGB1+/+ MEFs were starved in the presence or absence of 10 µM U0126 for 3 h. Protein expression levels were then assayed as indicated by co-IP or Western blotting.
Western blot of Csf-1r+/- endothelial cells treated with unconditioned, Csf-1r+/- MCM or PLX3397 MCM.
E. Percentage of oriented divisions for U2OS cells grown in subconfluent cultures. An oriented division axis was defined as being less than 30° removed from the long axis of the interphase cell. Mean of 50 cells per genotype are plotted, n=2. Error bars, SEM. ***, P< 0.0001; ANOVA.
c, Autophagosome candidate overlap from biological replicate experiments for PANC-1 and MCF7 cells, as well as overlap between PANC-1 and MCF7 data sets.
D, A549 cells were incubated with vancomycin at various concentrations and infected with HIV-luc/2019-nCoV pseudoviruses. The intracellular luciferase activity was measured at 48 h post-infection. Ordinary one-way ANOVA test was used for this analysis. Left: Chemical structure of vancomycin
(E) Stiff or soft B16 or MP-1 cells (100 or 10 cells) were injected into the NSG mice by tail veil. n = 8-12. Data information: Two-tailed Paired Student's t test The data represent mean ± SD.
A Exponentially growing cell cultures were serially diluted (1:10) before being spotted out onto YEPD plates with or without CPT at the indicated concentrations
(A) During starvation, Rab11-positive recycling endosomes are recruited to the SNX18-positive perinuclear area dependent on SNX18 itself. SNX18 and Rab11 are required for the perinuclear localization of Atg16L1 to omegasomes marked by DFCP1. LC3 lipidation and autophagosome formation proceeds. In the absence of SNX18, recruitment of recycling endosomes to the perinuclear site is inhibited, Atg16L1 fails to localize to DFCP1-positive omegasomes, and WIPI2 accumulates. LC3 lipidation and autophagosome formation is inhibited. Upon SNX18 overexpression, Rab11-, TfR-, and LC3-positive membranes accumulate at a perinuclear site, and extensive membrane tubules with SNX18, Atg16L1, LC3, and TfR form from perinuclear recycling endosomes. LC3 lipidation and autophagosome formation is increased. Based on the experimental observations summarized in this model, we propose that SNX18 facilitates LC3 lipidation via Atg16L1 and provides membrane from recycling endosomes to forming autophagosomes.
D Representative picture of 14-day-old plants grown 9 days in liquid 1/2MS supplemented with 1 µM flg22. The scale bar representing 0.6cm is included in the picture.
F-G, shows flow cytometric analysis of iNOShi (G) TAMs in MC38 and KPC tumours 5 days following IR. Data are presented as mean ± SEM and analysed by one-way ANOVA with Tukey post-hoc adjustment (n=6 mice/group).
C. A coronal HE-stained section (center) flanked by anatomically-corresponding coronal T2-weighted MR images of Mpdz-/- P18-P21 mice. Arrows or arrowheads show the match between the lateral ventricle CP in the HE section and in the MR images.
(A) qPCR measurements of circSLC8A1 and SLC8A1 mRNA after SH-SY treatment of statins or the PF LRRK2 inhibitor, T-test p**=0.007 and p**=0.01 for PF-06447475 and p*=0.047 and p=*0.0136 for Statins for cirSLC8A1 and SLC8A1 mRNA respectively
A Example confocal images from 10 min time-lapse imaging of control (GFP) or caldendrin (cald-GFP) transfected rat primary hippocampal neurons (DIV17) expressing an ER-marker (ER-tDimer). Yellow arrows show dendritic protrusions with a stably inserted ER (>10 min). Blue arrows indicate protrusions with a transient ER entry. Scale bar = 5 µm. B Quantification of dynamic SER presence in protrusions of control (GFP) or caldendrin (cald-GFP) transfected neurons. In protrusions of cald-GFP neurons, the percentage of stably localized ER (> 10 min) is increased, while transient SER visits are decreased compared to the control. (mean ± SEM, 2-way ANOVA, * p=0.0386 for interaction, n GFP = 9, n cald-GFP = 10; 2 experiments)
RAW 247.6 macrophages expressing cytosolic G-GECO1.2 (Cyto GG), TPC2-G-GECO1.2 (TPC2-GG) or TRPML1-G-GECO1.2 (TRPML1-GG). (F) Single-cell Ca2+ traces upon FcγR activation by opsonized 3-μm or 6-μm beads in -Ca2+o expressed as a percentage of the maximum indicator fluorescence (% Fmax = 2 μM ionomycin and 10 mM CaCl2). (G) Summary of first Ca2+ spike; n = 69-121 (3-μm bead), 22-46 (6-μm beads) cells; ***P<0.001, **P<0.01 vs Cyto GG, ### P<0.001 TPC2-GG vs TRPML1-GG.
Effect on NMDAR-subunit promoters. Cells transfected with pGluN1 or pGluN2A were treated and analyzed as in panel A (pGluN1, *p = 0.016; pGluN2A, *p = 0.029; n = 14).
L. p4xDPCSUMO was subjected to sequential addition of NPE and CSF-arrested extract as in (I). CSF-arrested extracts from (K) were supplemented with recombinant RNF4, and the DPC plasmid was recovered by DPC pull-down at the indicated time points and immunoblotted against M.HpaII.
Polysome fractionation analysis of OXPHOS mutants. Polysomes were fractionated by sucrose gradients (10%-50%), and the gradients were separated and measured at A254.
C. Confocal images of U2OS cells transiently expressing lifeAct-GFP wild type and mutants of lifeAct residues interacting with F-actin, cells were additionally stained with SiR-actin to confirm the actin filaments. The line scan as indicated with yellow line on the cells shows the extent of lifeAct (green) and SiR-actin (magenta) co-staining of actin structures. Scale bar = 5µm.
(F) Neuron-specific knockout of atgl-1 causes LD accumulation in neurons, similar to atgl-1(xd314) mutants. Scale bar: 5 μm. (G) Quantification of the number of LDs in head neurons in different genetic backgrounds. Each dot represents one worm. The data were analyzed using one-way ANOVA with Dunnett's multiple comparison test. Asterisks denote significant differences as compared to the control xdIs109. t-test was used between the groups under the crossbars. ns: not statistically significant. ****P < 0.0001. Data show mean ± SEM. n ≥ 9
C) Metacore enrichment scores of the seven (7) modules initially related to brain size variation across all subjects. Each module is called by its assigned color and represents the top process network obtained by the enrichment analysis in Metacore GeneGO (see also EV1).
A. Conservation of phosphorylation sites for LATS1/2 and NLK. Amino acid sequences of different species of YAP, Yorkie (in Drosophila), and TAZ (YAP paralog) were aligned. NLK is MAPK (mitogen-activated protein kinase)-type kinase, which prefers proline-directed motif (S/TP).
(C-E) Quantification of paternal/maternal (C) 5mC-, (D) 5hmC-, and (E) 5caC-signal ratios of 12 hpf derived zygotes normalized against DNA signal. A total of 5-16 zygotes from two to three experiments per condition were analyzed. Significance to control zygotes was calculated using ordinary one-way ANOVA with Tukey's multiple comparisons test (C, E) or Welch ANOVA with Dunnett's T3 multiple comparisons test (D) and significant differences are indicated (* = p < 0.05, ** = < 0.01, *** = p < 0.001, **** = p < 0.0001, data are represented as mean ± SD, with dots representing single zygotes).
The GAS-luciferase reporter activity assay was performed in Stat3-/- MEFs transfected with empty vector (EV) or STAT4 constructs and then treated with LIF for 8 hours.
A. In situ hybridization. G4C2 RNA foci were detected in a C9 patient-derived fibroblast but not in a control-derived fibroblast with non-targeting siRNA treatment (left panels, control/si Ct. middle panels, C9/si Ct). Knockdown of EXSOC10 increased RNA foci (right panels, C9/siEXOSC10#5). Nuclei were stained with DAPI (blue). White arrows indicate nuclear RNA foci. The green arrows show cytoplasmic RNA foci.
(B and C) 4T1-injected BALB/c mice were sacrificed at the indicated week after initial injection. Total cells isolated from inguinal lymph node tissues were transwell co-cultured with 4T1 cells. Inguinal lymph node tissues came from un-injection BALB/c mice as control. After 5-day co-culture, 4T1 cells at lower well were examined in the Western blotting (C) analyses of Il-17rb expression. Gapdh was used as an internal control or as a loading control.
d. Representative flow cytometry assay of mito-QC ARPE-19 ULK1 CRISPR KO cells with/without re-introduction of FLAG-ULK1, treated with DFP for 24 h. A decrease/increase in GFP/mCherry expression was used quantified to measure the percent of cells undergoing mitophagy (indicated by number in each panel). e. Quantitation of the flow data representing mean of n=2 ± SEM. Statistical analysis was performed with one-way ANOVA and a Tukey's multiple comparisons test. ****=P< 0.0001.
(D) Examples of stem cell and stemness signatures which were commonly higher expressed in the single knockout Hoxa9/- LSK-MLL-ENL cells compared to Prmt1 down-regulated or β-catenin inactivated Hoxa9-/- LSK-MLL-ENL cells.
I-L Plots of SOX9 index (total, HSC/Hep, and biliary) and NI score marking progressors in green and non-progressors in red. The solid line indicates the mean in each graph. The broken line in NI dot plot (L) indicates the threshold (score = 8) below which all NI is categorized as 'mild'.
Upon ligand binding (androgen, A), the androgen receptor (AR) interacts with an androgen-response-element (ARE) in the promoter region of the RNA editing gene ADAR2, promoting its repression. This in turn increases circFNTA levels, which leads to elevated sponging of the microRNA miR-370-3p and increased expression of FNTA. High levels of FNTA activate KRAS to promote cell invasion and chemo-resistance (chemo-R).
(C) Comparison of tRNA modification profiles among P. falciparum, S. cerevisiae and H. sapiens. tRNA wobble modifications are highlighted in red, with wobble-specific modifications bolded and underlined. The assignments of tRNA modifications to H. sapiens and S. cerevisiae are based on published work (Cantara et al., 2011, Chan et al., 2010, Jühling, Mörl et al., 2009, Kellner, Neumann et al., 2014, Machnicka et al., 2013, Phizicky & Hopper, 2010, Su et al., 2014)
Centrinone-treated pcnt KO, c5rap2 KO and akap KO cells, were incubated with NZ for 3h, fixed and double labelled for GM130 and either CDK5Rap2 or PCNT as indicated (n=3).
A. All protein N-termini were grouped according to the identity of their N-terminal residues and sorted in ascending order of stability.
(B) The interaction of LMP1 and endogenous ANT1 was analyzed by an immunofluorescence confocal assay. HK1-LMP1 cells were immunostained with anti-LMP1 (green) and anti-ANT1 (red) antibodies and then subjected to confocal microscopy，and use imageJ to calculate the co-location level of both. The nuclei were stained with DAPI (scale bar, 5 μm). The quantified graph on the top right shows the percentage of yellow fluorescence (merge) to red fluorescence (ANT1) in HK1-LMP1 cells. Data are presented as means ± S.E.M. (n = 6, biological replicates per group).
A, B) Infectious virus titers can be observed by virus titrations on VeroE6 cells of apical washes at 2, 24, 48, and 72h after infection at MOI 0.01 (A) or 0.1 (B) with SARS-CoV-2 (red). The dotted line indicates the lower limit of detection. Error bars represent SEM. N=4. H p.i. = hours post infection.
Percentage of embryos with or without MLS phenotype in the different conditions in cox7b-MO (N) embryos. N≥300 embryos/conditions.
Number of single amino acid variants detected by whole exome sequencing and DNA, by RNA-Seq at the mRNA and by mass spectrometry at the protein level as well as confirmed candidates by validation using synthetic peptide spectra comparisons. It is apparent that only a very small fraction of all variants detected at the DNA or RNA level can be detected at the proteome level using current technology.
G. BAZ2A binding correlates the best with eigenvector values. Heat map showing Pearson correlation values of ChIPseq data sets with the eigenvector values from HiC data in ESC+serum and ESC+2i, respectively.
(D) NADH/NAD+ ratios were measured in cells grown under the same conditions as for biofilm formation. 100% corresponds to a NADH/NAD+ ratio of 0.09. Data are expressed as average ± SD of four independent experiments performed by duplicates. * Denotes p < 0.05, and *** denotes p < 0.001 compared to WT via one-way ANOVA with Dunnett's posttest.
(B, Flow-cytometric analysis of bone marrow and spleen from Pax5Jak2-KD/+ (black; n = 11) and control Pax5+/+ (grey; n = 7) mice at the age of 6-8 weeks. The frequencies of the indicated B cell types are shown for each organ (B). The data are presented as mean percentages with SEM and were statistically analyzed by multiple t-tests (unpaired and two-tailed with Holm-Šídák's correction); ns (P > 0.5), *P < 0.05, **P < 0.01.
(e) In cultured neurons, viability was tested by MTT assay after 3-MA (2.5 mmol l−1 at reperfusion) and thioperamide was administered in OGD/R (n=7 per condition; ***P0.001, with ANOVAs followed by the Bonferroni/Dunn post hoc test).
(H) Relative qRT-PCR analysis of ZIKV RNA in NS-1-inducible THP-1 cells treated with or without doxycycline (Dox), then left untreated or treated with Ac-YVAD-cmk (20 μΜ) for 3 hrs followed by ZIKV infection (MOI=1) for the indicated time points.
B) Bar graphs representing the number of NeoAggpS403 only (bars filled in light orange), NeoAggpS409 only (bars filled in light purple) and double phosphorylated NeoAggpS403-409 (unfilled bars) for each condition. Individual patients are labelled in different colored dots (n=3 biological replicates for each condition, graph bar showing mean ± SEM). Two-way ANOVA repeated measures, with post-hoc Fisher's LSD test, F(24.54)=3.799, p<0.0001, Day 4: non-neurodegenerative control pS403/404 vs pS409/410, p<0.0001, non-neurodegenerative control pS403/404 vs bi-phosphorylated, p<0.0001, FTLD-TDP-A pS403/404 vs pS409/410, p<0.0001, FTLD-TDP-A pS403/404 vs bi-phosphorylated, p<0.0001 FTLD-TDP-C pS403/404 vs pS409/410, p<0.0001, FTLD-TDP-C pS403/404 vs bi-phosphorylated, p<0.0001. Day 5: non-neurodegenerative control pS403/404 vs bi-phosphorylated, p=0.0219, FTLD-TDP-A pS403/404 vs double, p=0.0650, FTLD-TDP-C pS403/404 vs double, p=0.0157. Day 6: Control bi-phosphorylated vs FTLD-TDP-A bi-phosphorylated, p=0.5096, control bi-phosphorylated vs FTLD-TDP-C, p=0.1010. Non-neurodegenerative control pS403/404: day 3 vs 4, p=0.0559, day 3 vs 6, p=0.0369. FTLD-TDP-A pS404/404, day 3 vs 4, p=0.0075, day 4 vs 5, p=0.0714. FTLD-TDP-C pS403-404, day3 vs 4, P=0.0759, day 4 vs 6, 9=0.0305. FTLD-TDP-A bi-phosphorylated: day 3 vs 5, P=0.0577, day 3 vs 6, p=0.0609. FTLD-TDP-C bi-phosphorylatede: day 3 vs 4, p=0.0522.
C57BL/6 mice were treated with G-CSF, CoPP or solvent controls (NaCl, DMSO) for 5 consecutive days. Samples were collected 6 hours after the last injection. (A) Representative flow cytometry plots show higher granularity (SSC) and Ly6G expression in CoPP-mobilized granulocytes than in G-CSF mobilized granulocytes.
K. (Left) Representative image of CENP-B box (red) staining after siRNA against CENP-A/HJURP for 48 h. CENP-B box signals at individual micronuclei are magnified in insets. Scale bar represent 5 µm. (Right) Bar graph represents the mean of the CENP-B box FISH intensity contained in the main nucleus versus the one present in the micronucleus ± SEM. Color dots represent independent experiments. (n > 43 micronuclei and 840 main nucleus) Mann-Whitney U test: ***p = 0.0001.
(b) Defect of cerebellar growth in Miz1ΔPOZNes mice. The panels show sagittal sections of brains of control and Miz1ΔPOZNes mice at the indicated age. Dotted lines show the circumference of the cerebellum. For each mouse, the cerebellar surface area was determined; numbers show the ratio between control and Miz1ΔPOZNes mice. The number of mice analysed for each genotype is indicated on the right. Scale bar, 1 mm.
(B) NLAD8∆Vpu-infected CD59 KO primary CD4 T cells were cultured with indicated antibodies and NHS or HIHS for 24h. Numbers indicate the percentage of infected (Gag+) cells. One representative experiment is shown.
G Isolated human pDCs were treated with 10 μg/ml of 24F4A (black line), 24F4A-ef (gray shaded histogram), or the isotype control (gray dotted line) for 10 min at 37°C. Flow cytometry was used to evaluate pSYK and pPLCγ2. Shown is a representative plot of two independent experiments.
(C and D) Representative immunoblots (C) and densitometric quantification (D) of whole-cell acid extracts of wild-type and Δach1 cells combined with or without knockdown of ACS2 (tet-ACS2). Cells were chronologically aged to day 3 in the presence of 1 ng/ml doxycycline. Blots were probed with antibodies against total histone H3 (loading control) or H3 acetylated lysines (K9Ac, K14Ac, K18Ac). Data represent means ± SEM (n = 8).
Mice were pre-treated with pemafibrate (1mg/kg) or vehicle (0,9% NaCl) for 1 week before being subjected to CLP. Plasma was collected 24h post-surgery and creatinine concentrations, were measured as described in methods. P-values were calculated with 2-way ANOVA tests. n = 6-7/group, combined data of 2 independent experiments.
E) Calibration curves for the different sensor-fusion proteins. Ratiometric response of sEcGFP to the local pH. Extracellular buffers with different pH values used: MES (2-(N-morpholino) ethanesulfonic acid, 20 mM, pH 5.6-6.1), BES (N,N-bis(2-hydroxyethyl)-2-aminoethanesulfonic acid, 20 mM, pH 6.1-7.6) or HEPPSO (4-(2-hydroxyethyl)-piperazine-1-(2-hydroxy)-propanesulfonic acid, 20 mM, pH 7.1-8.0) in 125 mM KCl, 20 mM NaCl, 0.5 mM CaCl2, 0.5 mM MgSO4 plus FCCP (10 µM), nigericin (1 µM) and oligomycin (5 µg/mL) Each data point represents n=2 independent experiments and mean values ± s.d. of at least 15 cells. NFO: nigericin + FCCP + oligomycin; NFOD: nigericin + FCCP + oligomycin + digitonin; NFOT: nigericin + FCCP + oligomycin + Triton X-100.
(a) Co-immunostaining for Cx43 and the indicated Atgs in NRK cells maintained in the presence or absence of serum (top). Single and merged channels of the 3D reconstruction of the outlined regions are shown (bottom). Nuclei are highlighted with DAPI.
(B) Relative expression of the top 20 up-regulated circRNAs from the GEO dataset GSE92675 (BCa tumor tissues compared to para-tumor tissues) in response to AR modulation in J82 and UMUC3 cells. The # indicates an RNA with a relatively low expression (qRT-PCR cycle number > 35), thus the circRNA was excluded for further analysis.
Genomic DNA analysis of Tam‐treated mb1‐CreERT2 or mb1‐CreERT2;Sykfl/flmice (as indicated). Upper row: amplification of floxed (fl) and wt (+) alleles. Middle row: amplification of the deleted (d) allele. Lower row: loading control (TC21, a gene unaffected by the deletion of Syk). Established control DNA was used to visualize the individual bands (as indicated).
(J) Boxplots of GDH1 expression in four stages in the TCGA-KIRC cohort. In the boxplot, medians, 0.25/0.75 quantile, and min/max were represented by the central lines, the box limits, and the whiskers, respectively.(I: n = 267, II: n = 57, III: n = 123, IV: n = 84). One way ANOVA test was used with **p < 0.01.
Immunoblot analysis of anti-FLAG IP. Cells were fractionated 72 h post-transfection, and 3xFLAG constructs were immunoprecipitated from non-nucleosomal fraction and eluted with FLAG peptide.
D. Time-dependent cleavage of UBE2N-Ub by MvcA. UBE2N-Ub was incubated with MvcA at the molar ratio of 128. Samples taken at the indicated time points were resolved by SDS-PAGE and detected by CBB staining.
B The frequency of gut specific somatic insertion sites in the Pros>2XGFP and Delta>nlsGFP genetic backgrounds.
D Representative endogenous colocalization analysis of CCDC50 and NLRP3 in PMA-induced THP-1 cells treated with LPS and ATP. Scale bar, 10 μm.
J. qRT-PCR analysis of representative co-upregulated and co-downregulated genes in (G) at day 5 of neural differentiation.
(D) GFP-SLD5 mouse ES cells were treated with p97i as indicated and then fixed and stained with Hoechst. Arrows indicate accumulation of GFP-SLD5 on constitutive heterochromatin (53 % cells, n = 125).
Quantification of HA levels in non-reducing condition showed in (E). (n=3 independent experiments)
(E) Western blot analysis of LAMP1 and LAMP2 protein levels in WT and separate clones of STX16KO HeLa, Huh7, and U2OS cells.