Figure 6Action of the CP1-WT peptide in treatment of S. Typhimurium oral infection. (A) Survival for symptoms were monitored. Data information: For all the three infection models stated above, n = 2 biological replicates with 7 mice per cohort were used. representative data for only one biological replicate are presented as mean ± SD values for the parameters obtained from seven mice in each cohort. In panel A, percent survival was compared between untreated cohort (N.T) and the DMSO/peptide/ciprofloxacin-treated cohort using a two-way ANOVA test using Bonferroni correction (ns P > 0.05; * P < 0.01). The notations, namely, N.T denote no treatment; Pep and Cip denote CP1-WT and ciprofloxacin treatments respectively.Action of the CP1-WT peptide in treatment of S. Typhimurium oral infection. C-E Bacterial loads in S. Typhimurium infected organs, namely, (C) liver, (D) kidney and (E) spleen, were estimated. Data are presented as mean ± SD CFU/mL values for the bacterial organ loads isolated from seven mice in each cohort. Data information: For all the three infection models stated above, n = 2 biological replicates with 7 mice per cohort were used. representative data for only one biological replicate are presented as mean ± SD values for the parameters obtained from seven mice in each cohort. In panels, (C-E), Mann-Whitney rank sum test was used (ns P > 0.05, * P < 0.05, ** P < 0.01, *** P < 0.001). The notations, namely, N.T denote no treatment; Pep and Cip denote CP1-WT and ciprofloxacin treatments respectively.Action of the CP1-WT peptide in treatment of S. Typhimurium oral infection. F-J Histopathology analyses with representative H&E-stained liver sections at different magnifications are shown for S. Typhimurium infected liver treated with various agents. K Mean pathological scoring from the tissue sections used in histopathology analyses in panels F-J. Data information: For all the three infection models stated above, n = 2 biological replicates with 7 mice per cohort were used. In panels K to assign histopathological scores, data are collected from five mice in each cohort, and the representative data for only one biological replicate are presented as mean ± SD values. Mann-Whitney rank sum test was used (ns P > 0.05, * P < 0.05, ** P < 0.01, *** P < 0.001). The notations, namely, N.T denote no treatment; Pep and Cip denote CP1-WT and ciprofloxacin treatments respectively. For histopathology analyses in panels (F-J) except for uninfected and peptide-treated mice, pathology sections from infected or treated (DMSO or ciprofloxacin) mice showed aggregation of multiple inflammatory cells (INF) and edema (E) in liver parenchyma, central vein congestion (C), distortion of hepatic portal vein (HPV), hemorrhage (H), necrosis (N) and pyknotic nuclei (arrows in panels H, Scale bar: 50µm in panels (F-J)Action of the CP1-WT peptide in treatment of A. baumannii intraperitoneal infections. L-N Bacterial loads in A. baumannii infected organs, namely, (L) liver, (M) kidney and (N) spleen, were estimated. Data are presented as mean ± SD CFU/mL values for the bacterial organ loads isolated from seven mice in each cohort. Data information: For all the three infection models stated above, n = 2 biological replicates with 7 mice per cohort were used. In panels (L-N) representative data for only one biological replicate are presented as mean ± SD values for the parameters obtained from seven mice in each cohort. Mann-Whitney rank sum test was used (ns P > 0.05, * P < 0.05, ** P < 0.01, *** P < 0.001). The notations, namely, N.T denote no treatment; Pep and Cip denote CP1-WT and ciprofloxacin treatments respectively.