Figure 3A. Quantification of total number of follicular cells in germaria with follicular stem cells expressing normal levels of Dronc (DroncKO/+), without Dronc expression (DroncKO/DroncKO- Suntag-HA-Cherry), and expressing a catalytically inactive form of Dronc (DroncKO/DroncKO- FL-CAEA-Suntag-HA-Cherry ). The genotypes are as follows: 109-30-Gal4 (BL7023)/+ (n=13). 109-30-Gal4 (BL7023)/+; DroncKO Tub-G80ts (BL7019) / UAS-flippase (BL8209) DroncKO-FRT Dronc-GFP-Apex FRT-Suntag-HA-Cherry (n=15). 109-30-Gal4 (BL7023)/+; DroncKO Tub-G80ts (BL7019) / UAS-flippase (BL8209) DroncKO-FRT Dronc-GFP-Apex FRT-Dronc FL-CAEA-Suntag-HA-Cherry (n=14). A one-way ANOVA and Dunnett's multiple comparison post-test was used to determine statistical significance (***p≤0.001; ****p≤0.0001). Data information: Experimental flies were kept after eclosion from the pupae for 14 days at 29°C prior to dissection (applicable to all panels). All the experimental data shown have been obtained from N ≥ 2 biological replicates. The median and quartiles are indicated in the violin plots. All the quantifications were made in germaria containing one single group of germline cells wrapped by follicular cells.B. Quantification of total Castor-expressing cells within the FasIII cellular domain in the genotypes shown in A. n numbers are shown in A. A one-way ANOVA and Dunnett's multiple comparison post-test was used to determine statistical significance (****p≤0.0001). C. Percentage of Castor-expressing cells versus the total number of Follicular cells in germaria of the genotypes indicated in A. A Kruskal-Wallis test and Dunn's multiple comparison post-test were used to determine statistical significance (****p≤0.0001). Data information: Experimental flies were kept after eclosion from the pupae for 14 days at 29°C prior to dissection (applicable to all panels). All the experimental data shown have been obtained from N ≥ 2 biological replicates. The median and quartiles are indicated in the violin plots. The box plots show the median, first quartile, and third quartile of datasets. The whiskers illustrate the range between the maximum and minimum values of datasets. All the quantifications were made in germaria containing one single group of germline cells wrapped by follicular cells.D. Quantification of total number of follicular cells within the FasIII cellular domain in the following genotypes: 109-30-Gal4/+; Tub-G80ts (BL7019)/+ (n=16). 109-30-Gal4 (BL7023)/UAS-DriceRNAi UAS-DecayRNAi (a gift from Pascal Meier); UAS-DammRNAi, UAS-Dcp1RNAi (a gift from Pascal Meier) (n=15). 109-30-Gal4 (BL7023)/+; Tub-G80ts (BL7019)/ UAS-Dcp1RNAi (BL28909) (n=25) 109-30-Gal4 (BL7023)/+; Tub-G80ts (BL7019)/ UAS-DriceRNAi (BL32403) (n=16) 109-30-Gal4 (BL7023)/UAS-Dark-sh; Tub-G80ts (BL7019)/+ (a gift from M. Miura) (n=18). 109-30-Gal4 (BL7023)/UAS-microRNA-RHG; Tub-G80ts (BL7019)/+ (a gift from Iswar Hariharan) (n=10). A one-way ANOVA and Dunnett's multiple comparison post-test was used to determine statistical significance (***p≤0.001; ****p≤0.0001). Data information: Experimental flies were kept after eclosion from the pupae for 14 days at 29°C prior to dissection (applicable to all panels). All the experimental data shown have been obtained from N ≥ 2 biological replicates. The median and quartiles are indicated in the violin plots. All the quantifications were made in germaria containing one single group of germline cells wrapped by follicular cells.E. Quantification of total number of Castor-expressing cells in germaria of the genotypes indicated in D. n numbers are shown in D. A one-way ANOVA and Dunnett's multiple comparison post-test was used to determine statistical significance (***p≤0.001; ****p≤0.0001). F. Percentage of Castor-expressing cells versus the total number of Follicular cells (FasIII+ cells) in germaria of the genotypes indicated in A. A Kruskal-Wallis test and Dunn's multiple comparison post-test were used to determine statistical significance (n.s.= not significant, *p≤0.05, **p≤0.01, ****p≤0.0001). Data information: Experimental flies were kept after eclosion from the pupae for 14 days at 29°C prior to dissection (applicable to all panels). All the experimental data shown have been obtained from N ≥ 2 biological replicates. The median and quartiles are indicated in the violin plots. The box plots show the median, first quartile, and third quartile of datasets. The whiskers illustrate the range between the maximum and minimum values of datasets. All the quantifications were made in germaria containing one single group of germline cells wrapped by follicular cells.