Figure 4A-B. Representative 2D projections of confocal images showing the expression of Ci-155 (blue and grey channels), ptc-GFP (green and grey; ptc-GFP is a bona-fide transcriptional read out of Hh-pathway and weak hypomorph allele and Castor (red and grey) in germaria with either normal (A) or deficient Dronc expression (B). Genotypes: 109-30-Gal4 (BL7023)/ptc-GFPCB02030 (a gift from Isabel Guerrero) (A) and 109-30-Gal4 (BL7023)/ptc-GFPCB02030; DroncKOTub-G80ts (BL7019)/ UAS-flippase DroncKO-FRT-Dronc-GFP-APEX-FRT-QF. Data information: Scale bars represents 10 µm in the entire figure. Experimental flies were kept after eclosion from the pupae for 14 days at 29°C prior to dissection (applicable to all panels).D. Western blot showing Caspase-9 expression (upper lane) and actin (bottom lane, loading control) in either control or Caspase-9 deficient OVCAR-3 cells (24h and 72h post-transfection of an shRNA against Caspase-9). Notice the strong downregulation of Caspase-9 72h after siRNA treatment.H. Quantification of total number of follicular cells (left) or Castor-expressing cells (right) within the FasIII cellular domain in the following genotypes from left to right: Dronc -/- = CTRL= 109-30-Gal4 (BL7023)/; DroncKOTub-G80ts (BL7019)/ UAS-flippase (BL8209) DroncKO-FRT-Dronc-GFP-APEX-FRT-QF (n=16). CTRL= 109-30-Gal4 (BL7023)/UAS-smoAct (BL44621); DroncKOTub-G80ts (BL7019)/+ (n=11). Dronc -/- = 109-30-Gal4 (BL7023)/UAS-smoAct (BL44621); DroncKOTub-G80ts (BL7019)/ UAS-flippase (BL8209) DroncKO-FRT-Dronc-GFP-APEX-FRT-QF (n=8). CTRL= 109-30-Gal4 (BL7023)/UAS-Ci (BL28984); DroncKOTub-G80ts (BL7019)/+. (n=7). Dronc -/- = 109-30-Gal4 (BL7023)/UAS- UAS-Ci (BL28984); DroncKOTub-G80ts (BL7019)/ UAS-flippase (BL8209) DroncKO-FRT-Dronc-GFP-APEX-FRT-QF (n=21). A Kruskal-Wallis test and Dunn's multiple comparison post-test were used to determine statistical significance (n.s.= not significant; ****p≤0.0001;). Data information: Experimental flies were kept after eclosion from the pupae for 14 days at 29°C prior to dissection (applicable to all panels). Unless otherwise indicated, all experimental data presented were obtained from N ≥ 2 biological replicates. The median and quartiles are indicated in the violin plots. All the quantifications were made in germaria containing one single group of germline cells wrapped by follicular cells.J. Quantification of total number of follicular cells (left) or Castor-expressing cells (right) within the FasIII cellular domain in the following genotypes from left to right: Dronc +/+ = ptc-Gal4 (BL2017)/+; Tub-G80ts (BL7019) (n=19). Dronc +/- = ptc-Gal4 (BL2017)/+; DroncKOTub-G80ts (BL7019)/+ (n=23). Dronc +/- UAS-Dronc = ptc-Gal4 (BL2017)/+; DroncKOTub-G80ts (BL7019)/UAS-Dronc (BL56198) (n=14). A one-way ordinary ANOVA and Welch's correction were used to establish statistical significance (n.s.= not significant; ****p≤0.0001). Data information: Experimental flies were kept after eclosion from the pupae for 14 days at 29°C prior to dissection (applicable to all panels). Unless otherwise indicated, all experimental data presented were obtained from N ≥ 2 biological replicates. The median and quartiles are indicated in the violin plots. All the quantifications were made in germaria containing one single group of germline cells wrapped by follicular cells.