Figure 6A. Heat map of differentially expressed transcription factors in HRMECs in response to 500 nM PGF2α (n=3).B. Relative expression levels of FOS in retinal microvascular ECs from patients with PDR (n=4) compared with the corresponding expression levels from normal subjects (n=7) (Gene Expression Omnibus Database dataset GSE94019). TPM stands for "Transcripts per kilobase million." Data information: n.s. stands for "not significant." Data were analyzed by Mann-Whitney test (B, Data in B are expressed as the mean + SD.C. Effect of FOS knockdown on 500 nM PGF2α-induced CXCL8 expression in HRMECs (n=4). D. Effect of FOS knockdown on 500 nM PGF2α-induced HRMEC CXCL8 secretion in the culture medium (n=4). Data information: n.s. stands for "not significant." Data were analyzed by Mann-Whitney test C, D, Data in C, D, are represented as mean ± SEM. E. and F. Effect of RA (1 μM, AP-1 dimer inhibitor), SN-50 (100 μg/mL, NFκB inhibitor), and Inca-6 (2.5 μM, NFAT inhibitor) on 500 nM PGF2α-induced CXCL8 expression in HRMECs and HRMEC CXCL8 secretion in the culture medium (n=4). Data information: n.s. stands for "not significant." Data were analyzed by Mann-Whitney test E, F, Data in E, F, are represented as mean ± SEM. G. Representative images of scratch-induced migration of 500 nM PGF2α-treated HRMECs with or without FOS knockdown. The yellow solid line indicates the original position of the scratch, the dashed line indicates the position of the cells after migration, and the arrows represent the migratory direction. H. Quantitation of the cell-migration distance in H (n=4). I. Effect of FOS knockdown on 500 nM PGF2α-induced HRMEC proliferation (n=4). Data information: n.s. stands for "not significant." Data were analyzed by two-way ANOVA with Tukey's multiple comparisons test (H, I Scale bar: 100 μm (G) Data in H, I, are represented as mean ± SEM. J. Representative images of tube formation by 500 nM PGF2α-treated HRMECs with or without FOS knockdown. K. Quantitation of total tube length, number of junctions, number of meshes, and percentage of mesh area in J (n=4). Data information: n.s. stands for "not significant." Data were analyzed by two-way ANOVA with Tukey's multiple comparisons test K, Scale bar: 200 μm (J, Data in K, are represented as mean ± SEM. L. Fos mRNA levels in retinas from OIR mice during postnatal days 12-17 (n=3-4). Data information: n.s. stands for "not significant." Data were analyzed by two-way ANOVA with Tukey's multiple comparisons test L, Data in , L, are represented as mean ± SEM. M. Effect of PTGFR deficiency in ECs on Fos expression in the retina from OIR mice on postnatal day 16 (n=6). Data information: n.s. stands for "not significant." Data were analyzed by Mann-Whitney test M, Data in M, are represented as mean ± SEM. N. Effect of FOS inhibitor SR11302 (1 μM) on 500 nM PGF2α-promoted sprouting of mouse aortic rings. Data information: Scale bar: 200 μm N).O. Quantitation of sprouting areas in N (n=6). Data information: n.s. stands for "not significant." Data were analyzed by two-way ANOVA with Tukey's multiple comparisons test O). Data in O, are represented as mean ± SEM. P. Effect of FOS inhibitor SR11302 (1 μM) on 500 nM PGF2α-promoted CXCL1 secretion in mouse aortic ring culture medium (n=6). Data information: n.s. stands for "not significant." Data were analyzed by Mann-Whitney test P) Data in P are represented as mean ± SEM.